RNAseq of mus musculus: normal muscle
Total RNA was isolated from tumors and normal muscle using Direct-zol RNA Miniprep Kit (Zymo Research). RNA samples were sequenced at the Duke Center for Genomic and Computational Biology Shared Resource. Total RNA was sequenced using paired end 150 base pair reads. 100 million reads were sequenced per sample in triplicate on a NovSeq instrument.