GSM7927581: MGMT_3x_control_rep3; Homo sapiens; OTHER
Cells were detached with 0.25% Trypsin-EDTA and DNA was extracted using the QIAamp® DNA Mini Kit according to manufacturer's instructions. Briefly, the extracted DNA was sheared, end-repaired with NEBNext Ultra II DNA Library Prep Kit and custom adapters were ligated. Fragments containing O6‑methylguanine were enriched with immunopercipitation and stalling of a high-fidelity polymerase was used to mark the site of O6‑methylguanine. Subtractive hybridisation was used to remove unwanted fragments and the library was indexed and amplified using dual indexing primers for Illumina. O6‑MeG‑seq, a novel sequencing strategy to map O6‑methylguanine in the human genome