GSM6592371: WT3; Mus musculus; RNA-Seq
LV tissues of nTG, KO, TG, and TG/KO mice (3 mo of age) were homogenized in TRIzol for RNA isolation with the Direct-zol RNA Miniprep Kit (Zymo Research). Total RNA (200 ng) underwent rRNA depletion with the NEBNext rRNA Depletion Kit followed by library construction with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs).