RNA-Seq of Mus musculus: aortic intima
Total RNA (100 ng) from mouse intima samples was used to generate barcoded libraries using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760, New England Biolabs). Polyadenylated RNA was first obtained using the NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490, New England Biolabs) and then fragmented. Following first and second cDNA strand synthesis, cDNA ends were repaired and adenylated. The NEBNext adaptor was then ligated, followed by second strand removal, uracil excision from the adaptor, and PCR amplification.