GSM6934723: I11; Homo sapiens; RNA-Seq
RNA was extracted using Trizol (Invitrogen). 1 ug of total RNA was used for the construction of sequencing libraries. Double-stranded cDNA was generated using the Superscript III First-Strand Synthesis System (Invitrogen, Carlsbad, CA). The cDNA products were used to construct libraries with the Nextera XT DNA Sample Preparation Kit (Illumina, Inc., San Diego, CA).