GSM6895301: GDM1_ATACseq; Homo sapiens; ATAC-seq
Cell permeabilization of native cells and tagmentation reaction with a Tn5 transposome for "Assay for transposase-accessible chromatin by sequencing (ATAC-seq)" according to Corces et al. (PMID: 28846090). Tagmented DNA was purified with HighPrep beads (Biozym) after guanidinium-mediated inactivation of the transposome according to Weichenhan et al. (PMID: 29224141). Libraries were prepared according to Weichenhan et al. (PMID: 29224141). Briefly, tagmented DNA was amplified by PCR under real-time conditions using a universal Illumina forward and a barcoded reverse primer and the NEBNext High Fidelity 2x PCR Master-Mix (NEB), and amplfication was monitored by inspecting real time the amplification curves. DNA was HighPrep bead-purified, Qubit (Invitrogen) quantitated and sized by TapeStation (Agilent) profiling.