GSM7871220: Ac, N , RIP-seq-N1; Amphidinium carterae; RIP-Seq
Total RNA was extracted from A. carterae using RNAzol®RT (MRC) according to the manufacturer's protocol.  Polyadenylated RNA (polyA+ RNA) was enriched from the total RNA samples by using two successive rounds of purification with VAHTS mRNA Capture Beads (Vazyme Biotech, Nanjing, China). m1A-RIP RNA library was prepared by VAHTS Universal V8 RNA-seq Library Prep Kit. For RNA-seq, the isolated total RNA from A. carterae cells (three biological replicates for all treatments) was sent to the Beijing Genomics Institute (BGI, Shenzhen, China) for strand specific mRNA library construction. For Ribo-seq, library was prepared using VAHTSTM Small RNA Library Prep Kit for Illumina.