GSM6622823: WT_2; Mus musculus; RNA-Seq
RNA was extracted using RNAqueousTM Total RNA isolation kit (Ambion) according to manufacturer instructions and quantified using Qubit 2.0 (Thermo Fisher Scientific) following manufacturer instructions. Libraries were prepared using the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina and sequenced with a NovaSeq 6000 instrument by the Deep-sequencing Facility at the Max Planck Institute for Immunobiology and Epigenetics.