GSM8179487: Cetuximab-sensitive LIM1215-S – biol rep 1 [S46]; Homo sapiens; RNA-Seq
Total RNA was extracted from frozen cell pellets with the Monarch Total RNA Miniprep kit (New England Biolabs) by following manufacturer's protocol. Total RNA (300 ng/sample) was ribodepleted by using the NEBNEXT rRNA Depeletion kit (New England Biolabs) according to manufacturer's instructions. Ribodepleted RNA was then used for the library preparation with the NEBNext Ultra II Directional RNA library prep kit for Illumina (New England Biolabs) according to manufacturer's instructions. Libraries were paired-end sequenced (2*100 base pairs) on a NextSeq2000 device (Illumina), with a minimal depth of 10 million reads.