GSM6934421: AZT-BNT-BNT-D3; Homo sapiens; RNA-Seq
CD4+ T cells were enriched by MACS (Miltenyi Biotec) and CD40L+4-1BB+ CD4+ T cells FACS sorted using an FACS Melody (BD). The cells were loaded with a maximum concentration of 1000 cells/µl and a maximum cell number of 17.000 cells on a Chromium Chip G (10x Genomics). Gene expression and TCR libraries were generated according to the manufacturer's instruction using the Chromium Next GEM single cell 5'Library and Gel bead Kit V1.1 and Chromium Single Cell V(D)J Enrichment Kit for human T cells (10x Genomics).