GSM7012497: 6M_GP_1; Mus musculus; RNA-Seq
Nucleospin RNA II kits was used for total RNA extraction.Subsequent denaturation by magnetic oligo (dT) beads was performed before the RNA sample purification. After the first-strand cDNA being obtained by reverse transcription of purified mRNA, and a second cDNA being synthesized, fragmented DNA samples were blunt ended and adenylated at the 3' ends. Adaptors were ligated to construct a library. Following purification, mRNA library was constructed, fragmented, amplified and loaded into the nanoarray and sequencing was performed on Illumina Novaseq 6000 platform following the vendor's recommender protocol.