GSM7286923: scBCR, adult male, subject P11, bone marrow, year 1 day-0, replicate 1; Homo sapiens; RNA-Seq
PBMCs were isolated from blood. Fine-needle aspiration was performed on axillary lymph nodes. RNA was purified from whole PBMCs and sorted PBs from participants using the RNeasy Micro Kit (Qiagen). Bulk BCR: Reverse transcription, unique molecular identifier (UMI) barcoding, cDNA amplification, and Illumina linker addition to B cell heavy chain transcripts were performed using the human NEBNext Immune Sequencing Kit (NEB) according to the manufacturer's instructions.  scBCR libraries were prepared as recommended by the 10× Genomics Chromium Single Cell V(D)J Reagent Kit (v1 Chemistry) user guide, with appropriate modifications to the PCR cycles based on the calculated cDNA concentration