GSM7011814: UTHBC0073 BA9; Homo sapiens; RNA-Seq
RNA was extracted from 50mg of BA9 tissue using RNeasy Plus Mini kits (Qiagen, Hilden, Germany) and RNA integrity number (RIN) was measured for RNA quality (Agilent Bioanalyzer 2100 system, Agilent Technologies, Santa Clara, CA). 1 μg RNA per sample was used for mRNA library construction using NEBNext® Ultra™ RNA Library Prep Kit (Illumina Inc, San Diego, CA). For cell samples: Cells were lifted in Trypsin, Accutase or 10 µM EDTA in PBS without calcium and magnesium, pelleted in 1.5mL tubes and stored at -80oC until use. Fibroblasts were pelleted at 1.5 million cells, iPSCs were pelleted from 2 wells of a 6 well plate at 60-80% confluence, NPCs were pelleted from 2 wells of a 6 well plate at 100% confluence, and neurons were pelleted from 2 wells of each 6 well differentiation plate. For cell samples: RNA was collected from dry frozen pellets using the RNeasy Plus Mini kits (Qiagen, Hilden, Germany). For Brain Tissue: RNA was extracted from 50mg of BA9 tissue using RNeasy Plus Mini kits (Qiagen, Hilden, Germany) and RNA integrity number (RIN) was measured for RNA quality (Agilent Bioanalyzer 2100 system, Agilent Technologies, Santa Clara, CA). For Cells: 400ng RNA per sample was used for mRNA library construction using NEBNext® Ultra™ RNA Library Prep Kit (Illumina Inc, San Diego, CA). Paired-end sequencing reads (150bp) were generated on an Illumina HiSeq2000 platform (Novogene Bioinformatics Institute, Chula Vista, CA). For Brain Tissue: 1 μg RNA per sample was used for mRNA library construction using NEBNext® Ultra™ RNA Library Prep Kit (Illumina Inc, San Diego, CA).