GSM6505634: PBS_RA_3; Rattus norvegicus; RNA-Seq
Whole lungs from normoxic and hypoxic (15% FiO2) rats sensitized with OVA (250ug) and challenged with either OVA or PBS were digested with collagenase A (1mg/ml) at 37C, processed into single cell suspensions, and RBCs were lysed with ACK lysing buffer. . 10x Genomics protocol for chromium single cell 3' reagent kits V3