GSM7012019: Cited2 overexpression, rep 3; Mus musculus; ATAC-seq
DRGs were dissociated using 1 mg/ml Dispase II, 2.5 mg/ml Collagenase Type II, and Papain. Released cells were placed into 15% BSA in DMEM gradient to obtain a neuronal enriched cell population Neurons were lysed to obtain nuclei, transposition reagents (Illumina) were added, DNA was purified using QIAGEN MinElute kit. Library was amplified (NEBNext HF 2x PCR Master Mix) with barcoded primers (Illumina). Library was purified using AMPure XP beads, followed by DNA elution.