GSM8305561: yak-2; Bos grunniens; RNA-Seq
Total RNA was extracted from each sample using TRIzol ( Invitrogen, Paisley, UK ). Nanodrop-2000 ( Thermo Fisher Scientific, Waltham, MA ) and 1 % agarose gel electrophoresis were used to evaluate the purity, concentration and integrity of total RNA. 1 μg total RNA was used to prepare cDNA library by cDNA-PCR sequencing kit ( SQK-PCS109, Oxford Nanopore Technologies, Oxford, UK ).