GSM6502539: tail Fltp+; Mus musculus; RNA-Seq
Pancreas head and tail were anatomically separated, followed by islet isolation with a standard protocol (Corbin et al., 2021; https://doi.org/10.1186/s12575-021-00143-x; D.-S. Li et al., 2009; https://doi.org/10.1038/nprot.2009.150). Islets from six FltpZV/+  mice were pooled. Fltp Venus reporter positive and negative cells were sorted. Libraries of single cells were produced using the Chromium Single-Cell 3′ library and 10XGenomics gel bead kit v2 (PN #120237). Briefly, 10,000 cells were loaded per channel of a 10x chip to produce gel bead-in-emulsions (GEMs). Then the samples underwent reverse transcription to barcode RNA followed by cleanup, cDNA amplification, enzymatic fragmentation, 5′ adaptor, and sample index attachment. The samples were sequenced using a HiSeq4000 (Illumina) with 150 bp paired-end sequencing.