GSM6506658: AL_ZT18_REP1; Mus musculus; RNA-Seq
Total RNA was extracted from 20 mg of liver tissue using RNeasy Mini Kit (Quagen, 74104) according to manufacturer protocol. RNA integrity and quantitation were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). A total amount of 1 μg RNA per sample was used as input material for the RNA sample preparations. After sample quality control, non-directional – polyA enrichment library was prepared using NEBNext Ultra™ II RNA Library Prep Kit for Illumina, and 20M reads (150bp, paired end) per sample were generated on Illumina NovaSeq 6000 platform. Sequencing libraries were generated using NEBNext® UltraTM RNA  Library Prep Kit for Illumina® (NEB, USA)  Library Prep Kit for Illumina® (NEB, USA)  Library Prep Kit for Illumina® (NEB, USA)  Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.