GSM6813478: PFC, female, ethanol, H3K36me3, 5; Mus musculus; ChIP-Seq
H3K36me3 (active motif #61101) and H3K9me3 (abcam #ab8898) ChIP was performed on prefrontal cortex tissue using the MAGNA ChIP Hi-Sens kit (Millipore, Burlington, MA), following the manufacturer's instructions. DNA was extracted using phenol:chloroform:isoamyl alcohol (Thermo Fisher, Waltham, MA) and purified with ethanol precipitation. Pellets were resuspended in 20uLTE buffer and stored at -20C. To obtain sufficient DNA for sequencing, 8 technical replicates from each sonicated sample were subjected to immunoprecipitation for each antibody. DNA from all 8 technical replicates were combined and concentrated using a speed-vacuum prior to library preparation. Preparation of libraries were conducted following standard protocols using the Accel-NGS 2S PLUS DNA Library kit (Swift).