GSM8387263: control_liver_rep1; Mus musculus; RNA-Seq
Total RNA was extracted from the liver, hippocampus and testis of control and CRS-treated mice using RNeasy Plus Universal Kits (Qiagen, Germany) according to the manufacturer's instructions. The concentration and quality of the total RNA were determined with a NanoDrop 2000 (Thermo Fisher Scientific, USA.) and a 2100 Bioanalyzer (Agilent, USA). A total of 5 μg of high-quality RNA was used to construct each sequencing library. The mRNA-seq libraries were prepared with a TruSeq Stranded Total RNA Preparation Kit (Illumina, CA) according to the manufacturer's instructions. cDNA synthesis, end repair, A-base addition and ligation of the Illumina-indexed adaptors were performed according to the Illumina protocol.