PacBio sequencing of SAMD00633373 : 16S amplicon analysis of oral microbiome FH15085
The full-length 16S rRNA gene was amplified using the following primers with the sample-specific 8-base tag sequence: 8F (5'-AGA GTT TGA TYM TGG CTC AG-3') and 1492R (5'-GGY TAC CTT GTT ACG ACT T-3'). PCR amplification was carried out using KOD DNA polymerase. The CCS reads with greater than or equal to 3 full-pass subreads and greater than or equal to 20 quality values were excluded from the analysis when they exhibited <1000 bases or did not include the correct forward and reverse primer sequences.