GSM7011967: Glioblastoma (G) [S5-2]; Homo sapiens; OTHER
Total RNA was extracted using either the Maxwell RSC simplyRNA Tissue Kit (Promega) with Maxwell RSC Instrument (Promega) configured with Low Elution Volume (LEV) hardware according to the manufacturer's protocol or TRIzol (Invitrogen) according to manufacturer's instructions.   Total RNA was partially and randomly fragmented under alkaline conditions. Libraries of 24 samples were prepared simultaneously using NEBNext Multiplex Small RNA Library kit (New England Biolabs). A calibrated source of total RNA (Human Xpress Ref Universal Total RNA, Qiagen) was introduced in each library.