GSM8389226: A172 cells, mock-irradiated, bio_rep3; Homo sapiens; RNA-Seq
Total RNA was extracted using RNeasy Mini Kit (Qiagen cat. No. 74104) and RNase-free DNase set (Qiagen, Cat. No: 79254), according to the manufacturer's instructions. RNA samples were run on an Agilent Tapestation 4200 to determine RNA quality, and all samples had a RIN score 8 or higher. One microgram of total DNAse-treated RNA was then used for library preparation with the TruSeq Stranded mRNA Library Prep Kit from Illumina. Polyadenylated RNA was purified and fragmented before strand specific cDNA synthesis.