GSM6934852: HEK293 ROR1- FBC selection rep 3; Oryctolagus cuniculus; RNA-Seq
0.5 mL of the bacterial panning output glycerol stocks was subjected to purified phagemid preparation using the QIAprep Spin Miniprep Kit (Qiagen). Rabbit VH domains were amplified via a two-step PCR procedure in which Illumina P5 and P7 adapters were added. Libraries were sequenced on a MiSeq sequencer (Illumina) using MiSeq Reagent Kit v3 (150-cycle) in a single end configuration with reads originating from the P5 primer binding site.