GSM7015718: Line_6_MG01_Rep_A4; Homo sapiens; RNA-Seq
Cryogenic vials were transferred to a 37°C water bath for ~2 minutes resuspended in either RPMI-1640 or StemPro-34 SFM medium in a drop-wise fashion. Cells were counted on the NC-200 and quenched with an additional 3mL of its respective medium. Cells were centrifuged at 250g for 5 minutes and resuspended in the appropriate assay or culture medium. Library was performed according to the manufacter's instructions (Single Cell 3' v3, 10x Genomics).