bulk RNA-Seq for RUNX1: variant RX1_32 (G100V), replicate A
We measured the impact of 10 RUNX1 Runt domain missense mutations in myelogenous leukemia cells (K562) with bulk RNA-sequencing using the 10X Genomics Chromium platform. WT and LOF controls were also included. Bulk screen was performed for each variant separately in K562 cells grown in doxycycline to induce repression of endogenous RUNX1  and hygromycin to select for transduced cells. Each variant contains three biological replicates with more than 1 million cells. At day 7 post transduction, bulk RNA libraries were prepared and sequenced.