Illumina MiSeq paired end sequencing; Whole Genome Sequencing yjhX mutant screen
Whole Genome Sequencing yjhX mutant screen
Colonies selected from mutant screen for overnight cultures grown in LB at 37C.   Bacterial cultures were grown at 37 °C in LB medium. 100 μl of an overnight culture was washed and plated on M9+0.2% lactose agar. Spontaneous survivors identified by whole genome sequencing Genomic DNA was prepared from overnight culture using a DNeasy Blood and Tissue Kit (Qiagen).  A DNA library was prepared using a Nextera kit (Illumina).