WGS_DEB125-1_iSC_r1
Fibroblasts were cultured in DMEM with 10%FBS. iPSCs were cultured in StemFit Basic03 with bFGF. iSCs were cultured in Defined Keratinocyte Serum Free Medium. Genomic DNA (gDNA) was extracted from cell pellets of skin fibroblasts, iPS cells, and derived keratinocytes from three patients (11 total samples) with the MasterPure Complete DNA Purification Kit (Lucigen #MC85200). The gDNA yield was quantified by the Qubit dsDNA high-sensitivity (HS) fluorescence assay. The gDNA purity was verified by the NanoDrop 2000 spectrophotometer (Thermo Scientific #ND-2000). Tagmentation-based sequencing libraries were prepared from 500 ng gDNA in duplicate with the Illumina DNA prep (M) kit (Illumina #20018704) using IDT® for Illumina® DNA or RNA UD Indexes Set B, Tagmentation (Illumina #20027214). Libraries were sequenced in 150-bp paired end format on two sequential NovaSeq 6000 S4 lanes (Novogene Corporation Inc.). Each sample obtained at least 40X average coverage after combining the data from both library replicates.