GSM6895892: NUP98/NSD1+ RUNX1/ZNF423+ AML blasts from patient, RNA-seq; Homo sapiens; RNA-Seq
Mononuclear cells derived from the patient were isolated from bone marrow using lymphocyte monocyte separation medium (GE Healthcare); Mononuclear cells were isolated from spleen of PDX mice using mouse mononuclear cell isolate kit (Solarbio) RNA was harvested using Rneasy mini plus kit (Qiagen). 1.3 ug of total RNA was used for the construction of sequencing libraries.