16S rRNA amplicon sequencing of iron microbial mats from the Savannah River Site wetland
DNA was extracted from iron microbial mat frozen in the field on dry ice. 16S rRNA gene libraries were prepared using the Illumina 16S Sample Preparation Guide. Amplicons were prepared using the lllumina 16S b341F and lllumina 16S 805R primers, with all preparations done at  volume. Libraries were prepared with 12.5 ng input DNA and 30 PCR cycles. Libraries were sequenced on an Illumina MiSeq using a Nano 500 cycle sequencing kit.