GSM7017033: Neonatal, replicate 1-2, scRNAseq; Mus musculus; RNA-Seq
Lungs from neonatal and adult Zbtb46-YFP mice were processed. Two replicates in the same group (NEO or AD) were stained with TotalSeq-B anti-mouse Hashtag1 and Hashtag2 (BioLegend), respectively. Cells were then stained with LIVE/DEAD AQUA, CD45.2, and DCs were purified by FACS sorting of live CD45.2 YFP+ cells. Single cell libraries were prepared according to the protocol for Chromium Single Cell 3' Reagent Kit v2 (10X Genomics). Briefly, sorted DCs from the same group of mice were combined. DCs, barcoded single cell 3ʹ gel beads and partitioning oil were then loaded onto a Single Cell A Chip, and single cell Gel Bead-In-EMulsions (GEMs) were generated by Chromium controller. Barcoded cDNA was then produced by the incubation of GEMs and amplified by PCR after the GEMs were broken to generate sufficient mass. Sequencing libraries were finally constructed with the P5 primer, sample index and P7 primer added.