GSM7016947: Day 14 cells, Vehicle group; Homo sapiens; RNA-Seq
Cells were harvested on day 0 and every 7 days from at least three pooled 96-wells from both the vehicle and TCDD treated experimental groups. Library was performed according to the manufacter's instructions (Single cell 3' v3 protocol (version 3.2), 10X Genomics). Briefly, cell suspension was loaded with partitioning oil and gel beads into the chip to generate the gel bead-in-emulsion (GEM). The cDNA libraries were subjected to quality control analysis including a double-sided size selection using Solid Phase Reversible Immobilization (SPRI) beads. DNA fragments were selected with a size range between 400-1000 base pairs. cDNA libraries were then sequenced on an Illumina HiSeq platform at an average sequencing depth of 50,000 paired end reads per cell.