GSM8178188: WT1 SIO + NKTs; Mus musculus; RNA-Seq
RNA was harvested using Rneasy mini kit (QIAGEN). RNA was then quantified using with Nanodrop and submitted for RNA-sequencing. Library preparation and whole transcriptome profiling was performed by The Francis Crick Institute Advanced sequencing facility. Sequencing libraries were prepared with 100ng total RNA with RN integrity (RIN) values of more than 7. The KAPA mRNA HyperPrep Kit for Illumina sequencing (Roche) was used to prepare the sequencing libraries following manufacturer's instructions. The quality and fragment size distributions of the purified libraries was assessed by a 4200 TapeStation Instrument (Agilent Technologies).