GSM6814955: Cas9 on mPB CD34 edited at day 3 collected at day 7 - rep. 7; Homo sapiens; OTHER
gDNA was isolated with QIAamp DNA Micro Kit (QIAGEN) according to the manufacturer's instructions. Custom library construction protocol. PCR amplicons for each individual sample were generated by nested PCR starting from >50-100 ng of purified gDNA. The first PCR step was performed with GoTaq G2 DNA Polymerase (Promega) according to manufacturer instruction using the following amplification protocol: 95°C x 10', (95°C x 30'', 63°C x 30'', 72°C x 30'') x 20 cycles, 72°C x 10'. The second PCR step was performed using the same reagents of the first step, 5 μl of the PCR product and the following amplification protocol: 95°C x 10'