GSM8003619: RIAILs_Batch4_QX538; Caenorhabditis elegans; RNA-Seq
On the day of the extraction, 1 mL of TriPure Isolation Reagent was added to each tube. The samples were then frozen and thawed quickly eight times with liquid nitrogen and 37ºC water bath to rupture cell membranes. RNA was then extracted by using a column-based kit from Macherey-Nagel. BGISeq-500 library construction Protocol