GSM6622151: kdm6a_cKO_cKIT_ATAC_3; Mus musculus; ATAC-seq
Dissociated germ cells were incubated in the dark for 30 mins with PE conjugated anti-cKIT at 1:800 (Thermo, 12-1171-82). Stained cells were then washed twice, resuspended to 10 million cells/ml and sorted on a two-laser (466 nm and 561 nm) cell sorter (Bio-Rad S3) as previously described (Vasiliauskaitė et al., 2018). 100,000 cells were pelleted for library prep. Libraries were prepared using the Active Motif ATAC-Seq kit (53150) according to the manufacturer's instructions except 11 PCR cycles were used instead of the recommended 10 cycles.