Nyctanthes Leaf sample
50 ng of Qubit quantified DNA was enzymatically fragmented, end-repaired and A-tailed in a one-tube reaction using the FX Enzyme Mix provided in the QIASeq FX DNA kit. The end-repaired and adenylated fragments were subjected to adapter ligation, whereby index- incorporated Illumina adapter was ligated, to generate sequencing library. The library was subjected to 6 cycles of Indexing-PCR (Initial Denaturation at 98C for 2 min, cycling (98C for 20sec, 60C for 30sec, 72C for 30sec) and final extension at 72C for 1min) to enrich the adapter-tagged fragments.Finally, the amplified library was purified using HighPrep TM beads followed by library quality control check. Illumina-compatible sequencing libraries were quantified by Qubit fluorometer and its fragment size distribution was analyzed on Agilent 2200 TapeStation.