GSM6592909: Aged/Control IgG treated Input; Mus musculus; ChIP-Seq
Epidermal cells were isolated by treating the skins with trypsin and generating a single cell suspension with mechanical chopping. Chromatin was isolated by detergent extraction+sonication of nuclear extracts. NEBNext Ultra DNA Library Prep for Illumina kit according to the manufacturer's protocol. All purification steps were performed using AgenCourt AMPure XP beads Input and ChIP enriched DNA were subjected to end repair and addition of “A” bases to 3′ ends, ligation of the NEB adapter and USER excision.