GSM8048786: M_KO_LPS_5 [645_M_KO_LPS_S29]; Mus musculus; RNA-Seq
RNA extraction was done using the Qiagen RNA isolation kit following the manufacturer's instructions (RNeasy® Mini Kit, 74104, Qiagen). A DNase treatment followed by a clean-up has been added using the RNA Clean & Concentrator™-5 kit (Zymo Research) following the manufacturer's instructions. Quantification of total RNA was performed using a N60 Nanophotometer® (Implen GmbH) and a Qubit 2.0 Fluorometer® (Thermo Fisher Scientific) with the Qubit RNA HS Assay Kit. The quality was assessed using a Fragment Analyzer System (Agilent Technologies) using an RNA Pico Chip (Agilent Technologies) (only samples with RQN≥7 were further analysed). The Illumina® stranded Total RNA Prep, Ligation with Ribo-Zero Plus kit Library preparation has been used to process libraries for RNA-sequencing according to Illumina's Reference Guide # 1000000124514 v00 June 2020, starting with 25 ng of total RNA. All libraries have been quantified using a Qubit HS dsDNA kit (Thermo Fisher Scientific) and the library quality check has been performed using a High Sensitivity NGS Fragment Analysis Kit on the Fragment Analyzer System (Agilent Technologies).