GSM8305448: Lung_Immune_AML3, scRNA-seq; Mus musculus; RNA-Seq
All lungs were perfused and enzymatically digested with Collagenase/DNase I mixture, single-cell suspensions were obtained and CD45 positive cells were isolated using the mouse CD45 MicroBeads (Miltenyi Biotech). Libraries were prepared for CD45pos cells from all samples using the Chromium™ Single Cell 3' Reagent Kits (v3.1, 10x Genomics). Single Cell 3' Reagent Kits (v3.1, 10x Genomics).