GSM6592102: Macaque_PFC_Hi-C; Macaca mulatta; Hi-C
0.2 g frozen PFC sample was ground in liquid nitrogen and prepared into cell suspension. The filtered cell suspensions were fixed in 1% formaldehyde for 30 min. The cross-linked DNA was digested with 200U MboI, and the digested fragment ends were filled with biotin-14-dATP, dCTP, dGTP and dTTP by DNA Polymerase I Klenow Fragment (NEB, #M0210L). The resulting blunt-end fragments were re-ligated by T4 DNA ligase. Then, the ligated cross-linked DNA was reversed by proteinase K. DNA purification was performed by removing biotin from unligated ends using T4 DNA polymerase. The resulting purified DNA was sheared to a length of 350~500 bp using Covaris M220 and biotin-labeled DNA was pulled down with Dynabeads M-280 Streptavidin (Invitrogen). The library preparation including end repair, adapter ligation and PCR amplification was performed using DNA library preparation kit (Vazyme, #ND607). The resulting libraries were sequenced by Illumina 150 bp paired-end sequencing. Hi-C libraries were prepared using VAHTS Universal DNA Library Prep Kit for Illumina® V3 (Vazyme, #ND607) following manufacturer's instructions.