GSM6731237: Wild-type cells, replicate 3; Homo sapiens; RNA-Seq
Panc-1 cells were lysed using Tri Reagent (Molecular Research Center, USA). Total RNA was treated with DNAse I (NEB, USA) and purified with RNA Clean & Concentrator (Zymo Research, USA). RNA-seq poli A enriched transcripts library preparation and sequencing were performed at BMR-Genomics (Padua, Italy) following Illumina specifications. RNA libraries for RNA-seq were prepared using TruSeq RNA Library Prep Kit v2 following manufacturer's protocols.