Cas12a CRISPRa Genome-wide tiling screen with p65 activator and wide design in MelJuso cells, dropout screen, set A, replicate B
MelJuSo cells containing 5x-tag-dCas12a-VP64 were transduced in two biological replicates with the lentiviral library.  Puromycin was added 2 days post-transduction and maintained for 5 days to ensure complete selection for transduced cells. Upon puromycin removal, cells were split to any drug arms (each at a representation of at least 1,000 cells per guide) and passaged on drug every 2-3 days for an additional 2 weeks to allow guides to enrich or deplete, at which point, 21 days post-transduction, cells were collected; genomic DNA from these cells was PCR amplified and sequenced