GSM8179565: WT-input; Mus musculus; ChIP-Seq
1% formaldehyde was used to crosslink proteins and DNA for 10min. Cell lysates were sonicated to obtain DNA fragments, then immunoprecipitated with 3 µg anti-H3K23pr at 4 °C for 12-16 hours. The complexes were rotationally incubated with 30 µL ChIP-Grade Protein G Magnetic Beads for 2hr at 4 °C. Chromatin Immunoprecipitation was performed with GenSeq® Chromatin Immunoprecipitation (ChIP) Kit (GenSeq Inc.) according to the manufacturer's instructions.The yield of ChIPed DNA was determined via Quant IT fluorescence assay (ThermoFisher), and sequencing libraries were generated with GenSeq® Rapid DNA Library Prep Kit (GenSeq Inc.) by following the manufacturer's manual.