GSM7287021: CD8-KO2(cKO2); Mus musculus; RNA-Seq
On the day10 of LCMV Cl13 infection, 2×106 CD8+ T cells were sorted by flow cytometry and collected. RNA was purified and concentration were then examined using NanoDrop 2000, RNA integrity and quantity were measured using the Agilent 2100/4200 system. The library was constructed using standard Illumina library construction process. After library construction, the concentration of library was measured by the Qubit fluorometer. The accurate concentration of cDNA library was again examined using qPCR. The size distribution of library was detected by agarose gel electrophoresis. After library preparation and pooling of different samples, the samples were subjected for Illumina sequencing.