GSM7802668: Liver, DIO-NASH-HCC, Rep 5; Mus musculus; RNA-Seq
Liver tissue (Chow mice, GAN DIO-NASH-HCC mice) and liver tumour samples (GAN DIO-NASH-HCC mice) were collected and snap-frozen in liquid nitrogen. RNA was isolated using the NucleoSpin® kit (MACHEREY-NAGEL). RNA sequence libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) and sequenced on NextSeq 500 (Illumina, San Diego, CA) with NextSeq 500/550 High Output Kit V2 (#75 CYS, Illumina)