Whole genome sequencing for de novo genome assembly of Arctocephalus gazella: skin tissue sample
A total of 115 mg of skin tissue was ground and incubated in a solution of 9.5 ml G2 DNA Enhancer, RNase A and Protease for lysis. A Qiagen HMW DNA extraction kit was then used to extract at least 21.0 g of DNA. In the extracted DNA, spooling was observed, and the DNA was dissolved in 100 l of TE Buffer. The extracted DNA was then used to prepare PacBio CCS with PacBio circular consensus sequencing (CCS) libraries, as well as to prepare Dovetail Omni-C libraries.