transcriptomics of rumen fluid of goats
Total RNA was extracted from rumen epithelium Trizol reagent (Tiangen Biotech, Beijing, China) according to the manufacturers protocol. RNA quality was determined by 5300 Bioanalyser Agilent, Palo Alto, CA, USA) and hight-quality RNA samples were sent for commercial library preparation and sequencing by Majorbio Biotech (Shanghai, China) on Illumina Novaseq 6000.