CRISPR-Cas9 mutation readout on the pathway reporters in replicate 2 : Sample from validation experiment in hTERT RPE-1 iCut DSB-TRIP cells treated with 1 nM PCI-24781
CRISPR-Cas9 mutation readout on the pathway reporters in hTERT RPE-1 iCut DSB-TRIP cells, 72 hours after activation the cells were collected and processed with two successive PCRs on the integrated pathway reporters to generate the libraries, spanning the gRNA target site and the barcode