GSM6622171: Pu-seq, RNASEH2A-mAID (the control line of POLE1-M630F cells), rep 3; Homo sapiens; OTHER
DNA was prepared using  the QIAGEN Blood & Cell Culture DNA Kits (100/G) 25 μg of genomic DNA was alkali treated in 0.3 M NaOH at 55 °C for 2 hr, then loaded onto a 1.5% agarose gel and run for 1 hr 40 min at 100 V. The gel was stained with acridine orange (final concentration 5 μg/ml) for 2 hr at room temperature with gentle shaking followed by overnight destaining in water. Fragments of 300–2000 bp were excised from the gel and isolated with a gel-extraction kit (Macherey-Nagel, NucleoSpin Gel and PCR Clean-up, #740609). Library preparation was performed as previously described in Daigaku et al Nat Struct Mol Biol 2015 (doi:10.1038/nsmb.2962).