16S rRNA amplicon sequence: microbial community structure in compost
DNA was extracted from composting samples using the Fast DNA Spin Kit for Soil (MP Biomedical, Solon, OH, USA). Each sample underwent three rounds of DNA extraction, and a NanoDrop ND2000 spectrophotometer (Thermo Fisher Scientific, Wilmington, USA) was used to measure the concentration of DNA. Primers  515F5-GTGCCAGCMGCCGCGGTAA-3/907R5-CCCCGYCAATTCMTTTRAGT-3 were used to amplify the 16S rRNA genes of bacteria. Majorbio Bio-pharm Technology Co., Ltd. in Shanghai, China used the Illumina Miseq PE300 platform to perform the sequencing. QIIME2 (Version 2022.2) was employed to perform demultiplexing, quality control, filtering, and merging of the initial reads. The processed sequences underwent denoising with the DADA2 plugin within QIIME90 to generate amplicon sequencing variants while eliminating all sequences associated with chloroplast and mitochondrial annotations.