Illumina Multiplexed Amplicon NGS
IDT's rhAmpSeq Design Tool for CRISPR gene editing analysis was used to design multiplexed primer pools. Libraries were generated using each primer pool and IDT's rhAmpSeq CRISPR Library Kit along with rhAmpSeq Index Primers. Samples were run according to manufacturers instructions.  Forward and reverse reads were merged with SeqPrep.  Reads were then demultiplexed followed by application of quality and amplicon filters.