GSM6510107: T14_Sal7; Rattus norvegicus; RNA-Seq
Fresh right lungs were immediately collected in RNAlater™ (Sigma, USA) for the next homogenization in QIAzol® Lysis Reagent (QIAGEN, Netherlands) with gentleMACS™ Dissociator (Miltenyi Biotec, Germany). RNA was extracted using the QIAcube robotic workstation with the miRNeasy Mini Kit (QIAGEN, Netherlands), including DNase digestion protocol. Libraries for massive parallel RNA sequencing (RNA-seq) were prepared with the QuantSeq 3' mRNA-Seq Library Prep Kit FWD (Lexogen) and sequenced, in two runs, with the use of an Illumina NextSeq500 platform, which generated at least 30 million reads/sample (75 bp Single End).