Plasmid_Seq of Enterococcus italicus SD1
The paired-end sequencing library of the plasmid was prepared using the QIAseq FX DNA Library kit (Qiagen, Hilden, Germany). The library preparation process was initiated with 200 ng of plasmid DNA for each sample (Kazi et al., 2021). The DNA was enzymatically cleaved into smaller fragments followed by a continuous end-repair step where an A is added to the 3 ends making the DNA fragments ready for platform-specific adapters ligation on both ends of the DNA fragments. A high-fidelity amplification step was carried out with HiFi PCR Master Mix to ensure maximum yields from limited amounts of starting material. The amplified library was analysed using High Sensitivity (HS) DNA chip according to the manufacturer's instructions in Bioanalyzer 2100 (Agilent Technologies) and finally, the libraries were sequenced on an Illumina  NextSeq 500 platform (2 x 150 bp chemistry)