GSM6592429: StageII-Ctrl-rep2; Homo sapiens; RNA-Seq
Total RNA was isolated by using Direct-zol RNA MiniPrep Kit. The NEBNext Ultra RNA Library Prep Kit (NEB England BioLabs, E7775) was used for RNA sequencing library construction. Fragmented and randomly primed 2 × 150 bp paired-end libraries were sequenced using the Illumina HiSeq X Ten system.