GSM8305393: IP_HA2_R; Mus musculus; RIP-Seq
Resting primary B cells were isolated from mouse spleens by negative selection using Mouse CD43 (Untouched™ B Cells; Invitrogen). Cells were activated in vitro to induce proliferation. AGO2 was immunopercipitated using an antibody against its HA epitope after which RNA was extracted and small RNA libraries prepared. The experiment was also performed in cells from animals not expressing the HA epitope, but no RNA was recovered. Therefore, libraries from those experiments were not prepared. 8 microRNA-Seq samples were pooled and sequenced on NextSeq 2000 P2 using QIAseq miRNA Library Prep and single-end sequencing small RNA seq