GSM7871084: Rpa135HTP_Rpa12dC_none_3; Saccharomyces cerevisiae; OTHER
UV stabilized protein-RNA interactions were purified using three step purification. Last purification was performed under denaturing conditions and RNAs associated with HTP tagged protein were partially truncated. Sequencing 3' adapter and 5' adapter were ligated while protein-RNA complexes were bound to Ni-NTA agarose, RNA library was reverse transcribed and PCR amplified.