GSM7978037: wt ethanol 3; Mycolicibacterium smegmatis MC2 155; RNA-Seq
extraction protocol 3 (InnuPREP RNA Mini kit 2.0 – Analytik Jena) the following changes: lysozyme was incubated with the samples for 1h at 37 °C, when binding the total RNA onto spin filter R fresh 80% EtOH in RNAse free water was used and an additional step was added in before RNA elution with incubation of 7 µL DNAse with 50µL buffer for each sample at room temperature for 15min. In the final step, the samples were resuspended on 50µL RNAse free water. illumina stranded total RNA prep Ligation with Ribo-Zero plus (illumina)