Whole genome sequencing of Clostridioides difficile: patient stools
Genomic DNA was extracted with a genomic DNA extraction kit (Qiagen, USA) and quantified with Qubit. Whole genome sequencing was performed with massive parallel sequencing Illumina technology, A-tailed, ligated to paired-end adaptors and PCR amplified with a 500 bp insert. A mate-pair library with an insert size of 5 kb were used for library construction.