DNA-seq of P1 WWTP: shotgun metagenomics
The DNA extracted samples are fragmented using KAPPA fragmentation method to fragment the DNA into 600 bp length. The fragmented samples were processed for end repair and A-tailing with Hypa peep plus ERAT enzyme mix. Immediately after the end repair and A-tailing the adapter were added and ligated to the end repaired DNA fragments using DNA ligase. Library amplification was done to the adapter ligated samples with Illumina primers.