Shotgun metagenome of cow fecal slurry
DNA sequencing libraries were prepared from 50 ng of input DNA for each sample using the Illumina DNA prep kit with unique dual indexing as recommended by the manufacturer.  Library concentrations were determined using the Qubit 1X dsDNA High Sensitivity kit (ThermoFisher Scientific) and Qubit 2.0 fluorometer and average insert size was determined by sizing using the Agilent 2100 Bioanalyzer and a High Sensitivity DNA Analysis kit.  Libraries were pooled in an equimolar mixture and sequenced by the Georgia Institute of Technology Molecular Evolution Core on an Illumina NovaSeq 6000 instrument for 2  150-bp paired-end reads.  Adapter trimming and demultiplexing were carried out on the instrument.