Genome sequencing of Wolbachia strain wAlbA
The non-WGA sample was used to prepare an ONT library by shearing the DNA into ~8 kb fragments followed by purification and size-selection using AMPure XP beads (Beckman Coulter). The ONT library was then prepared with the Ligation Sequencing Kit (SQK-LSK109) and sequenced for 72 hours using a GridION (ONT) controlled by the MinKNOW software v20.06.9.