GSM7017256: ATAC-Seq Donor2_EGR2_KO; Homo sapiens; ATAC-seq
AAVS1 and EGR2 knockout PSMA CAR T-cells produced from two different healthy subjects were isolated from the in vitro “stress test” after multiple consecutive rounds of antigen stimulation. Dead cells were removed using the Dead Cell Removal Kit (Miltenyi Biotec), and PSMA CAR-expressing T-cells were enriched by magnetic cell separation using a Biotin-goat anti-mouse IgG F(ab)2 fragment (Jackson ImmunoResearch #115-065-072) and anti-biotin beads (Miltenyi Biotech). CAR T-cells were washed with PBS supplemented with 0.04 % BSA and nuclei were isolated according to the manufacturer's protocol (10x Genomics). Briefly, cells were incubated with lysis buffer for four minutes, washed three times and resuspended in chilled dilute nuclei buffer (10x Genomics). Absolute cell numbers were enumerated, and viability was determined using the trypan blue dye exclusion method.