GSM6895300: GDM1_ACTseqBS_H3K4me1; Homo sapiens; OTHER
ACT-seq-BS was done by combining ACT-seq (PMID: 31431618) and tagmentation-based whole genome bisulfite sequencing (TWGBS; PMID: 29224141) as follows. For the transposome, a methylated load adapter (PMID: 29224141) was generated. Cell permeabilization of native cells and tagmentation reaction with pA-Tn5 transposome-antibody complex for "Antibody-guided chromatin tagmentation (ACT-seq)" according to Carter et al (PMID: 31431618). Tagmented DNA was purified with MinElute (Qiagen) columns (ACTseq) according to the provider's instruction. After oligonucleotide replacement/gap repair, the bead-purified DNA was bisulfite-converted according to PMID: 29224141. Libraries were prepared according to Weichenhan et al. (PMID: 29224141). Briefly, tagmented DNA was amplified by PCR under real-time conditions using a universal Illumina forward and a barcoded reverse primer and the NEBNext High Fidelity 2x PCR Master-Mix (NEB), and amplfication was monitored by inspecting real time the amplification curves. DNA was HighPrep bead-purified, Qubit (Invitrogen) quantitated and sized by TapeStation (Agilent) profiling.