GSM7659065: Lg7742a transcriptome pool 1 ONT; Lemna gibba; RNA-Seq
Frozen whole frond tissue (100-150 mg) was lysed with Bashing Beads (Zymo) and total RNA was extracted with the ZR Plant RNA MiniPrep kit (Zymo) following manufacterer's instructions. DNase treatment was performed on-column. Polyadenylated RNA was enriched from total RNA with the Dynabeads mRNA Purification Kit (Life Technologies). Indexed, directional RNA-seq libraries were prepared with ScriptSeq v2 reagents (Epicentre) according to the manufacturer's instructions.