GSM6510193: CPZ-12+8wk-1 (F), C57BL/6, cortex, rep 1; Mus musculus; RNA-Seq
Mice were anesthetized followed by transcardial perfusion with 20mL of sterile PBS (Kim et al., 2012, Steelman et al., 2012). The brain was removed, and the corpus callosum, cortex, and hippocampus were microdissected. Each piece of tissue was flash frozen in liquid nitrogen and stored at -80°C until use. RNA was extracted with Tri Reagent. Messenger RNA sequencing libraries were prepared using the TruSeq Stranded mRNA library preparation kit (Illumina). Libraries were pooled and sequenced on a one hundred fifty  base pair, paired-end sequencing run on an Illumina NovaSeq 6000.