PBMC genomic DNA
BROCA-GO (4547) is a targeted next generation sequencing assay which identifies all classes of mutations in gynecologic cancer susceptibility genes, genes associated with HRR, and other genes frequently altered in gynecologic cancers. For BROCA sequencing analysis genomic DNA was isolated from 5 x 106 K562 SRSF2WT and SRSF2P95H cells to determine mutations in HRR pathway genes