GSM7016364: RNA-Sequencing from mouse primary microglia 5; Mus musculus; RNA-Seq
RNA was isolated using trizol and with Zymo RNA isolation kit. Total RNA was used for the library preparation using the TrueSeq RNA library prep kit v2 (Illumina, USA) according to the manufacturer's protocol. 500 ng RNA was used as starting material. The quality of the libraries was assessed using the Bioanalyzer (Agilent Technologies). Library concentration was measured by Qubit™ dsDNA HS Assay Kit (Thermo Fisher Scientific, USA). Multiplexed libraries were directly loaded onto a Hiseq2000 (Ilumina) with 50 bp single read setup.