Illumina WGS of Xanthomonas oryzae pv. oryzae: Genome Sequencing
Use the SDS or STE method to extract the genomic DNA of the sample, then use agarose gel electrophoresis to detect the purity and integrity of the DNA, and use Qubit for quantification. DNA samples that pass the electrophoresis test are randomly fragmented into fragments of approximately 350 bp using a Covaris ultrasonic fragmentation instrument. After processing the DNA fragments, use the NEBNextUltra DNA Library Prep Kit for Illumina (NEB, USA) to complete the entire library preparation through end repair, A-tail addition, sequencing adapter addition, purification, PCR amplification and other steps.