GSM6504882: 2aOKO-OL-Ribo-TRAP; Mus musculus; RNA-Seq
Single cell suspension from mouse brain tissue was prepared using Papain based Neural Tissue Dissociation Kit from Miltenyi Biotech. Total oligodendrocyte lineage cells were sorted based on GFP fluorescence signal using BD FACSAria II SORP Sorter (BD Biosciences). Library was performed according to the manufacter's instructions (single cell 3' v3 protocol, 10x Genomics). Briefly, single cells gel bead-in-emulsion (GEM) was generated using the isolated GFP positive cells. Barcoding, reverse transcription, cDNA amplification and subsequent library construction was performed according to manufacturers instructions.