GSM7802467: MSC_Ctrl_4; Mus musculus; RNA-Seq
RNA was extracted and isolated using the Single Cell RNA purification kit (Norgen) according to the manufacturer's instructions for bulk RNAseq experiments. For single cell experiments, sorted cells were loaded on a Chromium Controller using the Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 according to manufacturer's instructions. RNA sequencing libraries were prepared using the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Clontech/Takara) for bulk RNAseq and  Single-cell RNA-Seq libraries were prepared using Chromium Single Cell 3′ v2 or v3 Reagent Kit (10x Genomics) according to manufacturer's protocol.