microsporidian diversity in lake Saint-Gervais through metabarcoding
DNA was extracted using phenol-chloroform. Amplification of the V1V3 region of the microsporidian gene coding for the 18S rRNA was performed using the universal microsporidian V1F and 530R set. The amplicons were purified and concentrated using the QIAquick Gel Extraction kit. See Chauvet et al. 2023 for the detailed protocol. In this article, data from both size fractions (or dates for Roscoff) were grouped, leading to only one sample per site.