GSM7016829: lmo1_KO_MYCN_1; Danio rerio; RNA-Seq
Tumors were extractyed and EGFP+ cells were sorted. Total RNA was extracted using the QIAzol lysis reagent (Qiagen) and was cleaned using the RNeasy kit (Qiagen). Samples were treated with the TURBO DNase (TURBO DNA-free Kit; Ambion) and cleaned using the RNeasy MinElute Cleanup kit (Qiagen).Total RNA was depleted of ribosomal RNA with the SMARTer Universal Low Input RNA Kit (Clontech). Enriched mRNA was then applied to library preparation according to manufacturer's protocol (NEBNext Ultra). Strand-specific library construction and Illumina NextSeq 500 sequencing of paired-end 100-bp-long reads were performed. Library preparation, quality control, and next-generation sequencing were conducted by the Molecular Biology Core Facility of the Dana-Farber Cancer Institute following standard protocols.