thermotolerance
TF12 and CF12 cysts were hatched under axenic conditions as previously described 55. All manipulations were conducted under a laminar flow hood, and all tools were autoclaved at 121 C for 20 min. After decapsulation, cysts were transferred to new 50-mL Falcon tubes containing 30 mL sterile 35 g/L artificial seawater (Instant Ocean, Blacksburg, VA, USA) and cultured in a rotary incubator for 28 h at 28 C with constant light at approximately 27 E/m2/s. After 28 h, swimming nauplii instar II (mouth opened to ingest particles) were used for RNA-Seq.