GSM6894007: PCHiC_1h_bioRep1; Mus musculus; Hi-C
Cell nuclei were digested by HindIII restriction enzyme, followed by overhangs repair, in-nucleus ligation, and reverse cross-linking overnight. The ligated DNA was extracted using cold ethanol and sodium acetate Methods for Hi-C and PCHi-C libraries preparation were described in Schoenfelder S et al. J Vis Exp 2018 and He et al. Epigenetics Chromatin 2022. For PCHi-C, SureSelectXT Custom 3-5.9Mb (Agilent, 5190-4831) and SSEL TE Reagent Kit, ILM PE FULL Adaptor (Agilent, 931108) were used for enriching the Hi-C fragments that are associated with promoters. PCHi-C