GSM7898209: PBS_mIgG_3; Homo sapiens; OTHER
Cells were rinsed with 1X PBS, incubated in 0.05% trypsin for 1 minute, physically scraped off the plate, then washed twice in 1X PBS by centrifugation for 3 minutes at 600 rcf. Sequencing libraries were prepared for next-generation sequencing using a combination of the KAPA HyperPrep Kit and the NEBNext Multiplex Oligos for Illumina. Amplified libraries were quantified with Qubit and fragment size was assessed by TapeStation.