GSM8389130: MEF_Ctrl_1_rep1; Mus musculus; OTHER
Total RNA from either two cell passages of four different cell lines or eight representative hearts from the m.5024T mouse model was extracted with TRIZOL under mild acidic conditions to preserve tRNA charging and  subjected to oxidation and beta-elimination (Behrens and Nedialkova, 2022). tRNA-seq libraries were prepared using the mim-tRNAseq workflow (Behrens and Nedialkova, 2022) and sequenced on an Illumina NextSeq 500 platform.