Cancer tolerance to chromosomal instability is driven by Stat1 inactivation in vivo
Cancer tolerance to chromosomal instability is driven by Stat1 inactivation in vivo
Mice were sacrificed when ill, i.e. displaying signs of hematopoietic malignancies including weight loss, lethargic behaviour, anaemia and dyspnea. Malignancies were dissected out and homogenised using a 100 um tissue strainer. Homogenised cell suspensions were centrifuged and taken up in 10% DMSO and 10% FBS in RPMI and stored at -80 degrees Celsius until RNA isolation. RNA was isolated using the RNeasy mini kit (Qiagen) and RNA quality was examined using the Agilent 2100 Bioanalyzer (Agilent Technologies). Typically 300 ng of total RNA was used as input for enrichment by NEXTflex Poly(A) Beads (Bioo Scientific) followed by library preparation using NEXTflex Rapid Directional qRNA-Seq Kit (Bioo Scientific).