Sc-RNA seq of spleenic NK cells from WT mice
Splenic NK cells pooled from three mice of WT mice were enriched by FACS for library preparation and scRNA-seq was performed by Shanghai Biotechnology Corporation. The final library pool was sequenced on the Illumina NovaSeq 6000 instrument using 150base pair paired-end reads. Raw sequencing data were converted to FASTQ files and aligned to the mouse genome reference sequence (GRCH38). The 10X Genomics Cell Ranger (3.0.1 version) was used to demultiplex samples, process barcodes, and generate a digital gene-cell matrix from this data.