GSM8178790: SU-DHL-4-shYTHDF2-2-rep2; Homo sapiens; RNA-Seq
Zymo Direct-Zol RNA Microprep Kit for RNA-seq, Zymo Oligo Clean & Concentrator Kit for RIP-seq, Zymo RNA & Concentrator for MeRIP-seq. For RNA-seq, about 300 ng total RNA of each sample was used for library preparation according to the manufacture's protocol of KAPA mRNA HyperPrep Kit (Illumina Platforms). 10cycles of PCR amplification were performed. KAPA Pure Beads (Kapa Biosystems) were used for library purification. The quality of each library was verified using a Qubit fluorometer (Thermo Fisher Scientific) and further evaluated by the 2100 Bioanalyzer (Agilent Technologies). For RIP-seq, the libraries were conducted using the KAPA Stranded mRNA-Seq Kit (Kapa Biosystems) after depletion of ribosome RNA (rRNA). For MeRIP-seq, the libraries were conducted usign the KAPA Stranded mRNA-Seq Kit (Kapa Biosystems).