GSM6896554: D1_day7_CART; Homo sapiens; ATAC-seq
CAR-T cells were collected at day 7, and CD8+ CAR-T cell population was isolated by flow cytometry. The CAR-transduced T cells were detected by the expression of truncated NGFR. The purified T cells were resuspended with complete culture medium and incubated for 30 minutes at 37 degrees after supplemented with DNAse solution. The cells were washed with PBS once and cryopreserved in growth media with 10% DMSO. Sequencing analysis was performed with NovaSeq 6000 (Illumina). Library preparation was performed using standard Illumina protocols.