GSM7393678: Rep2, ASO-Ctr; Homo sapiens; RNA-Seq
RNA extraction was performed using the Direct-zol RNA Miniprep Kits (Zymo), according to the manufacturer's instructions. 80-130ng equal volum RNA each sample was used to prepare sequencing library. The cDNA library was prepared with NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #  E7490S) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB # E7760L), following the manual instruction.