GSM7870485: 4FM3, replicate1, RNA-seq; Mus musculus; RNA-Seq
Mice were sacrificed by CO2 inhalation, and hearts were immediately cannulated and perfused with cold PBS. After the heart was harvested, the atria and valves and right ventricure were removed, and  RNA was extracted using Trizol reagent. 500 ng total RNA is used for rRNA-depletion (NEB, E6310), followed by directional library synthesis (NEB, E7420)