DNBSEQ-G400 paired end sequencing; Gamma delta T cells isolated from spleen of cold-exposed rats against room-temperature placed control
Gamma delta T cells isolated from spleen of cold-exposed rats against room-temperature placed control
Gamma delta T cells from spleen were FACS sorted directly into the lysis buffer of a RNeasy® Micro Kit (QIAGEN, Hilden, Germany). RNA was isolated immediately according to the manufacturer's instructions. The cold acclimation was performed as previosly descibed Tibenska et al. (J. Appl. Physiol., 2021). Male Wistar rats were housed in pairs in well-bedded cages to minimize environmental and social stress. All experiments were performed in the “winter-spring” season (from October till March). Rats divided into two experimental groups were acclimated to 8 ± 1 °C for 1 day or 14 days. The control group was kept at 24 ± 1 °C. Gamma delta t cells from spleen were FACS sorted directly into the lysis buffer of a RNeasy® Micro Kit (QIAGEN, Hilden, Germany). RNA was isolated immediately according to the manufacturer's instructions. The isolated RNA was used for library preparation using the DNBSEQ Low Input Single Cell Smart-Seq Eukaryotic mRNA library preparation kit.