GSM6509914: Short-11 R2S5; Homo sapiens; ncRNA-Seq
100 μL LCL suspension containing approximately 6.6 x106 cells was taken through the Maxwell 16 miRNA tissue kit protocol using the Maxwell 16MDX instrument following the manufacturer's instructions, with miRNA eluted in 60 μL RNase-free water. RNA libraries for small RNA-seq were prepared using the Illumina TruSeq Small RNA library preparation kit following a modified protocol as described previously (Waller et al 2017 doi:10.3389/fnins.2017.00731). For cluster generation 1000 pM of each barcoded cDNA library were pooled together and loaded onto two lanes of the flow cell at 20 pM and 25 pM concentration per lane.