GSM8269454: control diet, replicate 2; Mus musculus; RNA-Seq
Mice were anesthetized with ketamine/xylazine and decapitated. Brains were removed, meninges were peeled off, and cerebellum and olfactory bulbs were discarded.  A series of enzymatic and mechanical steps was used to achieve a single cell suspension of the remaining brain tissue. FACS was used to enrich for CD31+ endothelial cells, which were sorted for single-cell sequencing. Brains from three mice were used across the two samples. Libraries were constructed with the 10X Genomics Chromium Next GEM Single Cell 3' kit v3.1 according to manufacturer's instructions