GSM7800699: child0803; Homo sapiens; Bisulfite-Seq
Details on genomic DNA extraction and storage were described in “Paltiel L, Anita H, Skjerden T, Harbak K, Bækken S, Kristin SN, et al. The biobank of the Norwegian Mother and Child Cohort Study – present status. Norsk Epidemiologi [Internet]. 2014 [cited 2023 Feb 1];24. Available from: https://www.ntnu.no/ojs/index.php/norepid/article/view/1755" 500 ng of genomic DNA were bisulfite converted and subjected to BRCA1 gene promoter fragment amplification using four pairs of primers, that do not overlap with any of the CpG dinucleotides (GRCh38 genomic coordinates: CpG00–13 chr17:43125624–43126026, CpG14–31 chr17:43125270–43125640, CpG17–34 chr17:43125171–43125550, CpG33–49 chr17:43124861–43125249). All four amplicons were combined, indexed, and sequenced by 2x226 bp reads using Illumina MiSeq System (Illumina, San Diego, CA), resulting in an ultradeep coverage of about 30000x for each amplicon.