16S RNA microbiota of human: infant stool: non-AAM
Microbial DNA was extracted using ZR Fecal DNA MiniPrep (Zymo Research) and purified with AMPure XP Beads (Beckman Coulter). The v3 - v4 region of the 16S rRNA gene was amplified using barcoded 341F and 805R primers. The PCR products were pooled at equimolar concentration, followed by adapter ligation using TruSeq DNA PCR-free LT Library Preparation Kit.