Lycorilla ingenua female ovaries RNAseq replicate 3
Adult flies were anaesthetised on ice, dissected in 1X phosphate buffered saline on a glass slide under a Leica EZ4 microscope, and flash-frozen at -70C. 20-60 individuals were pooled per replicate. RNA was extracted using a modified version of the PureLink RNA purification kit (ThermoFisher) with a TRIzol solubilisation step. All samples were quantified and quality-checked using the Qubit and Nandrop (ThermoFisher) and were sequenced for 30M 150bp paired-end reads on the NovaSeq 6000 platform.