GSM7050005: HepG2_SF24h_2; Homo sapiens; RNA-Seq
Total RNA was extracted using a miRNeasy mini kit with DNase treatment according to the manufacturer's instructions(QIAGEN, Germany). Libraries were prepared using total RNA lysates. Total RNA concentration and quality of the RNA were assessed with Qubit (Qubit™ DNA HS assay) and TapeStation 4150 with the RNA ScreenTape Assay  (Agilent Technologies Genomics, USA), respectively. RNA Integrity Number (RIN) values were assessed. Libraries were constructed from 100 ng of RNA with the Illumina Stranded TOTAL RNA prep Ligation with RIBO-ZERO PLUS. Libraries were amplified, purified and subjected to quality control with High Sensitivity DNA Qubit® Assay and High Sensitivity D100 ScreenTape Assay (average size 260-280 bp). Samples were then pooled together at 2.4 nM, diluted at 1.2 nM (PhiX included) and sequenced in NovaSEq6000 S1  instrument from Illumina (2x75 cycles)