Thai cynomolgus macaques genome project
6 ml of peripheral blood mononuclear cell (PBMC) was used to extract high molecular weight DNA and the long read DNA was submitted to Biomarker Technologies (BMKGENE) to perform long read sequencing. The experiments were processed following standard protocol provided by PacBio, including sample quality assessment, library preparation, library quality assessment and sequencing. Detailed steps in library preparation were listed as follows.1. DNA was fragmented with g-TUBE.2. Fragmented DNA was processed for nick repairing.3. Fragmented DNA was further processed for end repairing.4. SMRTbell adpater was ligated to the prepared DNA fragments.5. The ligation products were then treated by exonuclease to degrade failed ones.6. Fragments with designed size were selected by BluePippin and ready for sequence.