RNA-Seq of urothelial cancer
RNA sequencing was performed from samples from the clinical trial: Phase 2 Study of Pembrolizumab in Combination With Gemcitabine and Cisplatin as Neoadjuvant Therapy (NCT02690558). Sequencing libraries were prepared from 50 ng of the tumor RNA generated by the SMARTer Stranded Total RNA-Seq kit v2 (Takara).