GSM6502549: Mouse cortex, droplet-based snFFPE-Seq on 10X platform; Mus musculus; RNA-Seq
Nuclei were extracted from 50µm scrolls of FFPE mouse brain. Scrolls were first deparaffinized with mineral oil with heat, xylene with heat, or xylene at room temperature, then rehydrated with graded ethanol washes. Tissue were then lysed for nuclei extraction, and individual nuclei were sorted into 96-well plates. Library was constructed using SCRB-seq or SMART-Seq2, as described in the paper scRNA-seq via SMART-Seq2 and SCRB-Seq