GSM7179757: exosome from non-pretreated msenchymal stem cell, rep1; Rattus norvegicus; miRNA-Seq
Exosome was isolated from cell supernatants using the RiboTM Exosome Isolation Reagent (Ribobio, China), and Exosomal RNA was extracted by the MagZol (Magen) according to the manufacturer's protocol. Briefly, RNAs were ligated with 3'RNA adapter, and followed by 5'adapter ligation. Subsequently, the adapter-ligated RNAs were subjected to RT-PCR and amplified with a low-cycle. Then the PCR products were size selected by PAGE gel according to instructions of NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Illumina, USA). The purified library products were evaluated using the Agilent 2200 TapeStation, The libraries were sequenced by HiSeq 2500(Illumina, USA) with single-end 50bp at Ribobio Co. Ltd (Ribobio, China).