GSM8389345: pEC_WSE10_S19_Batch3; Homo sapiens; RNA-Seq
Co-cultures were treated with with eucalyptus wood smoke extract (10,30,50 mg/mL or 20,70, 100 mg/mL), lipopolysaccharide (LPS) (1 mg/mL), or LiCl (10 mM) for 24 hours. Cells were fixed with 4% paraformaldehyde (PFA) for ICC, lysed to collect mRNA for bulk-RNA sequencing, or supernatants were collected for ELISA and cytotoxicity assays. Total RNA was extracted from cell lysed in Qiagen Buffer RLT containing β-mercaptoethanol followed by RNA isolation using RNAeasy mini spin columns (Qiagen) Sequencing libraries were preparedusingIlluminaStranded mRNA prep, Ligation kit (Illumina)