GSM7871801: Kidney, Hmox1 KO, 20 h Heme treatment, rep 3; Mus musculus; RNA-Seq
Total RNA was extracted using mirVana™ miRNA Isolation Kit (ThermoFisher Scientific). Libraries were prepared from 500 ng of input material (total RNA) using NEBNext Ultra II Directional RNA Library Preparation Kit in combination with NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) following the manufacturer's instructions (New England Biolabs).