Whole Genome Sequencing using DNA Isolated from hair of Panthera tigris altaica:A8_TL
The study used the MGIEasy Universal DNA Library Preparation Kit from MGI, China, for the transformation of DNA samples into sequencable libraries. Notably, a direct end repair and A-tailing strategy was adopted, thereby bypassing DNA fragmentation. Moreover, the attached DNA adapters were PCR-amplified using 11 to 14 cycles, followed by gel recycling purification for targeted band sizes of 100-150 base pairs instead of typical DNA Clean Beads. The amplified products were subjected to electrophoresis (4.0% agarose, TAE1X, 80V, 75min) to ensure quality control. Finally, the PCR fragments were analyzed with the 5227 Fragment Analyzer by Agilent, USA, to assess DNA library quality.