GSM6774458: Schwann Cells, NF2-WT, Passage 7, Replicate 1; Homo sapiens; OTHER
genomic DNA was extracted using the QIAamp DNA Blood Maxi kit (#51192, Qiagen) A total of 140 PCR reactions were performed using 1μg of genomic or plasmid library DNA, Q5 Hot Start HF polymerase (#M0493L, NEB), and primers harboring the Illumina TruSeq adapter sequences. PCR products were purified using DNA Clean and Concentrator TM-100 (#C1016-50, Zymo Research) and MagSi-NGSprep Plus beads (#SL-MDKT-01500, Steinbrenner). Sample concentrations were measured using Qubit HS DNA Assay (#Q32851, Thermo Fisher). Library amplicon size was verified using DNA High Sensitivity Assay on a BioAnalyzer 2100 (Agilent) and then sequenced on a NextSeq (Illumina) by 75bp single-end sequencing and addition of 25% PhiX control v3 (Illumina).