RNAseq of copepods: SD females, high temperature, replicate 4
Adult copepods (~25-30 individuals) were placed in 5 ml artificial seawater in a 15 mL tube and immersed in a water bath at the target temperature (20C for control, 34C for high temp) for 60 minutes. Total RNA was isolated 20 minutes after return to control 20C incubator. Library was prepared with the NEBNext Ultra II Directional RNA kit, using 300 ng of total RNA as input.