C-1
The total RNA content were extracted from cells in the three groups with the Total RNA Extraction Kit (Qiagen, Valencia, CA, USA). The concentration and quality of extracted RNA were assessed using a NanoDrop spectrophotometer and an Agilent 2100 Bioanalyzer, respectively. After purification by RNA Clean XP Kit (Beckman Coulter, Inc. Kraemer Boulevard Brea, CA, USA), the RNA samples were subjected to RNA-seq. Briefly, the RNA library was constructed using an Illumina TruSeq RNA preparation kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions. The RNA samples were then sequenced on the Illumina NovaSeq6000 (Illumina, San Diego, CA, USA).