16S compost
Total genomic DNA from samples collected at T0, T2, T28 and T112 (n = 5) was extracted from subsamples stored at -80C using Qiagen's DNeasy Power Soil Pro kit according to the manufacturer's instructions. The quantity and quality of the extracted DNA was assessed with a Thermo Fisher NanoDrop 2000c. The V5-V6 region (based on Escherichia coli) of the 16S ribosomal RNA (rRNA) was selected for PCR amplification using the forward primer: P609D (5'- GGMTTAGATACCCBDGTA- 3') and reverse primer: P699R (5'- GGGTYKCGCTCGTTR-3')