GSM8174045: S06 Kras G12D d7 Male AAV-Cre on Kras HET genotype Liver; Mus musculus; RNA-Seq
Whole tissue RNA was extracted using the Qiagen RNeasy kit (Qiagen, 74104), including the optional DNase step, as described previously12. Briefly, 20-30mg of snap frozen tissue were homogenized in 600ml buffer RLT/1% β-mercaptoethanol using the Precellys Evolution homogenizer (Bertin Technologies) and the RNA was eluted in 30μ