GSM6896626: Morphine-1; Mus musculus; RNA-Seq
Total RNA was isolated from medial prefrontal cortex using RNeasy mini plus kit (Qiagen, Hilden, Germany). 3 μg of total RNA per sample was used for the construction of sequencing libraries. Ribosomal RNA was removed by the Ribo-Zero rRNA Removal Kit (Illumina, CA, USA) and sequencing libraries were generated using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA).