GSM6502792: MCF7 cells grown in medium from TAMs 3-2; Homo sapiens; RNA-Seq
Total RNA was purified from cells using the standard TRIzol method(Life Technologies) and the quality was determined by Bioanalyzer 4200(Agilent, Santa Clara, CA, USA) For RNA sequencing, the libraries were for all samples were simultaneously constructed using the VAHTS mRNA-seq v2 Library Prep Kit for Illumina® (Vazyme, Nanjing, China). The libraries were also analyzed by Bioanalyzer 4200 for quality control