GSM8268905: DMS273, Control, rep 1; Homo sapiens; RNA-Seq
Erdafitinib treated and untreated cells as above were used for RNA extraction after making cell pellets resuspended in 1 mL of TRIzol™ Reagent (Invitrogen) followed by phase separation using chloroform and vigorous shaking. RNA was precipitated used isopropranol (0.5ml per 1ml of Trizol reagen) and washed with 75% ethanol and centrifuged. RNA-seq libraries were generated using Truseq RNA Access Library Prep Kits (TruSeq RNA Exome kits; Illumina) and sequenced on Highseq3000 sequencers using 75 bp paired-end sequencing method (Illumina, San Diego, CA). For transcriptomic analyses, raw RNA-Seq count data were normalized for inter-gene/sample comparison using FPKM, followed by log2(x + 1) transformation, as implemented in the edgeR R/Bioconductor package