Iso-seq of Triticum Aestivum
Use Tiangen DP411 kit for RNA extraction. Purification of mRNA from total RNA using mRNA capture beads (Vazyme). Reverse transcriptase was used to synthesize the first strand cDNA and amplify double stranded cDNA. Using NEBNext FFPE DNA Repair Mix and NEBNext Ultra II End Repair/dA Tailing Module for damage repair and end repair. Use the SQK-LSK111 (ONT) reagent kit to complete the connection of the sequencing connector.