GSM7385393: HA-tagged WT RUNX1 Transduced HEL Cell CUT&RUN Rep 1; Homo sapiens; OTHER
500,000 cells for each sample were used to perform CUT&RUN. Cells were bound to magnetic Concanavalin A beads and permeabilized with 0.01% digitonin. The cell/bead mixture was incubated with a 1:100 dilution of anti-HA antibody at 4°C overnight. CUTANA pAG-MNase (EpiCypher) was used to cleave DNA fragments flanking the anti-HA bound sites. Sequencing libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645) per manufacturer's protocol. Illumina TrueSeq single indices are added during library preparation for sample multiplexing and each sequencing library was PCR amplified for 12 cycles. Libraries were size selected using magnetic AMPureXP beads. Sequencing was completed using standard Illumina protocols.