scRNA-seq of homo sapiens: organoid
Organoid cultures were established from patient biopsies and maintained in Matrigel (Corning) with Intesticult Human Organoid Growth Media (Stem Cell Technologies). Cells were encapsulated with a 10X Genomics Chromium, and after rt-PCR and cDNA amplification, libraries were prepared using 10X Genomics 3 single-cell gene expression chemistry and sequenced on Illuminas Novaseq6000 at the Yerkes Primate Research Center Genomics Core at Emory University.