RNA-seq data generated for the BarcUVa-Seq cohort: a cohort of normal mucosal biopsies.
RNA was extracted from frozen tissue using the mirVana kit (Thermo Fisher Scientific, Waltham, MA) after homogenization using the Minilys bead mill (Bertin Instruments, Montigny le Bretonneux, France). The RNA was DNAse treated and concentrated using the RNA Clean and Concentrator-5 kit (Zymo Research, Irvine, CA). Quantification of total RNA was executed using a Qubit Fluorometer (Invitrogen, Walthan, MA). An Agilent (Santa Clara, CA) 2100 Bioanalyzer or TapeStation was used to assess quality. For library preparation, the Illumina TruSeq Stranded Total RNA Library Prep Gold kit was used. Libraries were tagged with unique adapter indexes. Final libraries were validated on the Agilent 2100 Bioanalyzer, quantified via quantitative polymerase chain reaction, pooled at equimolar ratios, diluted, denatured, and loaded onto an Illumina HiSeq 2500 (high-output mode), for batches 1–7, or a NovaSeq 6000, for batch 8, instruments using a paired-end flowcell. Details can be found in DOI: https://doi.org/10.1016/j.jcmgh.2021.02.003