GSM6774755: CandR_H3K9me3_p5424_wt; Mus musculus; OTHER
CUT&RUN and CUT&Tag was performed using a magnetic bead-based digitonin-permabilization and an enzyme-tethering protocol respectively. Library preparation was carried out as per manufacturer's instructions (NEBNext Ultra II DNA library prep Kit for Illumina) using 5ng material with average sizes about 150-350 bp, including adapters. Sequencing was carried out on an Illumina NextSeq 500. The quality of libraries was checked with the Bioanalyser DNA High sensitivity.