GSM6732003: normal-720_input; Homo sapiens; RIP-Seq
Total RNA was isolated from tissues using the Magzol Reagent (Magen, China)according to the manufacturer's protocol. The  library was constructed using NEBNext® Ultra RNA LibraryPrep Kit for Illumina (NEB, USA).Briefly, RNA were fragmented to approximately 200bp. Then, the RNA fragments were subjected to first strand and second strand cDNA synthesis following by adaptor ligation and enrichment with a low-cycle.