GSM8268711: Infected_day0_mouse2; Mus musculus; miRNA-Seq
Faecal samples were stored at 4˚C prior to extraction of total RNA using the mirVana kit. 500ng total RNA per sample was used as input for library building with the NEBnext small RNA library preparation kit. SR adaptors and RT primers were undiluted. Amplification was performed with 12 PCR cycles. Size selection was performed using the Novex 6% TBE gel. small RNA sequencing