GSM8042306: Lesion 5.1 of ccRCC from the left kidney; Homo sapiens; RNA-Seq
Immediately after surgery, samples were harvested by a dedicated pathologist, flash-frozen in liquid nitrogen and then kept at -80°C before RNA extraction. RNA was extracted using a miRNeasy kit (Qiagen) according to the manufacturer's instructions. RNA purity was determined by NanoDrop spectrophotometer and integrity, and concentration of isolated RNA was assessed using the Tape Station 4100 (Agilent). RNA samples with RIN values <7 were excluded. RNA libraries for bulk RNA-seq were prepared using the Illumina Stranded mRNA kit starting from 100 ng of good quality RNA.