GSM6895279: Acetazolamide 10uM H69 3; Homo sapiens; RNA-Seq
RNA was isolated using QIAzol lysis reagent (Qiagen) according to the spin column miRNeasy Mini Kit (Qiagen) as previously used for total RNA isolation and downstream bulk RNA sequencing (GEO GSE156519). RNA concentration and quality were assessed by the Core Facility Genomics (Amsterdam UMC) using the RNA ScreenTape and RNA integrity scores (RIN) were 9-10. Three biological replicates per group were sent in for library preparation by KAPA mRNA Hyperprep. The RNA samples were enriched for mRNA by poly(A) mRNA capture with a library length from 150 – 850 base pairs.