GSM7392595: NK Pt09 Ex Vivo [RNA_S09]; Homo sapiens; RNA-Seq
Total RNA was extracted from the NK cell populations using a commercial PicoPure RNA Isolation Kit RNA-Seq libraries were generated as described in a previous study. Briefly, whole transcriptome amplification (WTA) and tagmentation-based library preparation was performed using SMART-seq2, followed by sequencing with a 75-cycle kit on a NextSeq 500 instrument (Illumina, CA). The quantification of transcript abundance was conducted using RSEM software (v1.2.22) supported by the STAR aligner software (STAR_2.4.2a). The raw reads were aligned to the Hg38 human genome database.