GSM7870827: Liver-derived ANKL1 cells of post-treated ANKL1-PDX; Homo sapiens; RNA-Seq
ANKL cells were obtained liver and spleen of treated and untreated ANKL-PDXs by digesting organs with 1 mg/mL of collagenase and 20 U/mL of DNase I and density gradient centrifugation using 35% Percoll. Finally, ANKL cells were stained with biotin-conjugated anti-human CD45 antibody and anti-biotin microbeads, following purification using autoMACS. Single cell cDNA library was constructed using BD Rhapsody WTA Amplification Kit and BD Hu Single-Cell Sample Multiplexing Kit.