K-mer analysis
The samples were randomly fragmented using the Covaris ultrasonic high-performance sample processing system, resulting in fragments of approximately 350 bp. Subsequently, DNA fragment end repair was performed, and after passing quality control, the samples were sequenced using high-throughput sequencing. For each qualified library, the raw image data obtained from sequencing were converted into raw sequence data (raw reads) in FASTQ file format. The SOAPnuke v2.1.0 software was employed for data filtering.