GSM8049019: E6.5_WT_2; Mus musculus; RNA-Seq
For low in put RNA-sequencing, RNA was isolated from indibidual epiblast usisng PicoPure RNA extraction kit (Thermo). Total RNA was isolated using Rneasy Mini Kit (QIAGEN) from EpiSCs. SMARTer® Stranded Total RNA-Seq Kit v2 was used for the library construction from epiblast sample. NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® was used for the library construction from EpiSCs sample.