GSM6896378: monkey_oocyte_GV_rep1; Macaca fascicularis; RNA-Seq
The oocytes were lysed in 72℃ and then put back on ice. Then the samples were spined down and reverse transcribed to cDNA based on polyA tail.  Switch the template at 5' end of the RNA and amplify the full-length cDNA by PCR. Quality control of the amplification products were performed using Agilent 2100 bioanalyzer instrument. The library was constructed following the Tagmentation-based library construction protocol. The double stranded PCR products were purified using the Agencourt AMPure XP-Medium kit and quantified by Agilent 2100 bioanalyzer. Next, the DNA were heat denatured and circularized by the split oligo sequence. The qualified single strand circle DNA were formatted as the final library. Finally, the library was amplified to make DNA nanoballs (DNB) which were loaded into the patterned nanoarray and the reads of paired-end 100 bases were generated in the way of sequenced by combinatorial Probe-Anchor Synthesis (cPAS).