GSM8339179: iPSC3; Homo sapiens; RNA-Seq
RNA was harvested using the RNAqueous micro kit (ThermoFisher). 500 ng of RNA was used for the construction of sequencing libraries. Illumina® Stranded mRNA Prep to enrich for mRNA using PolyA capture with ligation-based addition of adapters and indexes was used to make stranded libraries for Illumina sequencing. Twelve cycles of PCR were used to amplify the libraries according to manufacturer instructions.