GSM6507313: Screen 1, non-eaters, replicate 2 [S1_P5]; Mus musculus; OTHER
The sorted populations were processed individually for genomic DNA extraction using DNeasy Blood and Tissue Kit (Qiagen) and subjected to PCR reactions to generate the libraries, following the Library Amplification Protocol: https://www.addgene.org/pooled-library/broadgpp-mouse-knockout-brie/. The purified PCR products were sequenced on Illumina NextSeq500 system to determine gRNA abundance in two independent replicates, following the Sequencing Protocol: https://www.addgene.org/pooled-library/broadgpp-mouse-knockout-brie/.