GSM7050326: 6 ND27 Mlkl-/- #1154; Mus musculus; RNA-Seq
Total RNA extracted using Trizol RNA Isolation Reagents (Life Technologies) and purified using RNeasy Mini kit (Qiagen) according to the manufacturer's instructions. 3'mRNA-sequencing libraries were prepared using 100 ng of total RNA using the QuantSeq 3'mRNA-Seq Library Prep (Lexogen) according to the manufacturer's instructions and sequenced on the NextSeq 500 (Illumina). 3' mRNA-seq