GSM6814341: Str R2; Drosophila melanogaster; RNA-Seq
Total RNA was isolated using Trizol (Ambion) and the RNA Clean and Concentrator kit (Zymo Research). For reverse transcription of poly(A) mRNA, 500-1000 ng were used. Sequencing libraries were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina), following the manufacturer's instructions.