RNA Seq of Control H2122 SS cells
cells were seeded in triplicates in tissue-treated 12-well plates (Sarstedt) at a density of 1x105 cells/ml, in the absence (control cultures) or presence (treatment cultures) of human recombinant TGF-1 (at 10 ng/ml). After 24 hours, total RNA from control and treatment cultures was extracted using the Qiagen RNeasy mini kit, following the manufacturers instructions. For the treatment cultures, un-attached cells were removed before the adherent cells were trypsinized and harvested for RNA extraction. RNA quality control, library construction and sequencing were performed by Novogene.