GSM7872437: MCF10AT1_LA_rep2; Homo sapiens; ChIP-Seq
For ChIP, cells were cross-linked for 10 min with 1% formaldehyde, sonicated in a Diagenode Bioruptor to produce chromatin fragments of ~200 base-pairs; lysates were clarified by sedimentation and Lamin B1-DNA complexes were isolated from supernatants by ChIP using antibodies against lamin B1, washed and DNA was eluted for library preparation. For input samples, cells were crossed, sonicated and lysaates clarified as above, and DNA was purified. Diagenode Microplex library prepareation kit v2. TruSeq LT indexes, non-pooled samples.