GSM7179408: scRNA_endothelial_SuHX_Myl9Abs_rep1; Mus musculus; RNA-Seq
CD31+ endothelial cells recovered from the lungs of SuHx and control groups were stained with mouse PE anti-CD45 (103106, BioLegend), APC mouse anti-CD326 (118214, Biolegend), FITC mouse anti-CD31 (102405, Biolegend), and eBioscience Fixable Viability Dye eFluor 780 (65-0865-14, Invitrogen) for dead cell staining.  Live endothelial cells (CD31+, CD326-, CD45-) from mice were purified by BD FacsAria III (BD Bioscience) (10,000 cells each, cell viability >98%) and then encapsulated into droplets. Libraries were prepared using Chromium Single Cell 3' Reagent Kits v3.1 following the manufacturer's protocol (10X Genomics).  The generated scRNA-seq libraries were sequenced using a total of 127 cycles (paired-end reads) with a NovaSeq 6000 system (Illumina, Inc.)