GSM8174566: 24hAPF Eye RNA Rep3; Drosophila melanogaster; RNA-Seq
Dissected 16 eyes per sample in 1X PBS. Eye discs were separated from antenna discs during dissection. RNA was extracted using a standard Trizol/chloroform protocol, precipitated overnight at -20C in isopropanol, and quality was assessed with Agilent Bioanalyzer. Libraries were prepared using NEBNext Poly(A) mRNA Stranded Library Prep Kit and assessed on an Agilent Bioanalyzer prior to sequencing