Transcriptome sequence of Argopecten irradians SB127
RNA was extracted using the NucleoSpin RNA Plus RNA isolation kit (Macherey-Nagel, Dren, Germany). RNA quantity and quality were checked spectrophotometrically (NanoDrop ND-1000, Thermo Fisher Scientific, Wilmington, Delaware, USA). Library preparation and sequencing were performed by Novogene Corporation (UC Davis, Sacramento, California, USA). Sample quality control measures implemented by Novogene rely mainly on RNA Nano 6000 Assay Kit using the Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, California, USA). RNASeq libraries were prepared using 1 g RNA using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, Massachusetts USA). Library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, California, USA). Then 3 l USER Enzyme (New England Biolabs, USA) was used with size-selected, adaptor-ligated cDNA at 37C for 15 min followed by 5 min at 95C before PCR. Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. Finally, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using PE Cluster Kit cBot-HS (Illumina)