GSM6592896: Islets, Control, rep2; Mus musculus; RNA-Seq
Mouse islets were isolated as described in doi:10.1113/jphysiol.2003.059675 and doi:10.1038/s41467-018-03034-0. Islets were preserved in Trizol before their total RNA was extracted using Quick-RNA MicroPrep kit (R1050 Zymo Research). RNA libraries for RNA-seq were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina following manufacturer's protocols.