RNA-Seq of Botrytis cinerea B05.10 infecting A.thaliana leafs for 24h (12:12 L:O) plus an extra hour in dark
Total RNA extraction was performed with 10 mg of frozen plant-mycelia mixed tissue were ground to powder with TRizol reagent. RNA quantity and quality was accomplish with NanoDrop and a fluorescence-based capillary electrophoresis machine respectively. poly-A mRNA molecules were obtained and libraries were constructed using a TruSeq Stranded RNA Sample preparation kit