HiSeq X Ten paired end sequencing of SAMD00604070
The DNA fragments were sheared to 300 bp using Covaris Focused-ultrasonicator S220 (Covaris). NGS libraries were prepared using NEBNext Ultra II DNA Library Prep Kit (NEB) and NEBNext Multiplex Oligos for Illumina (NEB). These libraries were deep sequenced by a commercial company (Macrogen) using Hiseq X (2 x 150bp; Illumina).