GSM7015685: LC_2; Mus musculus; RNA-Seq
RNA was isolated from total lung tissue using the Qiagen RNA isolation kit according to manufacturer protocol. RNA was stored in 30µl of RNase/DNase free water and quantified using the NanoDrop 2000c (Thermofisher). Total RNA quality was assessed using the Tapestation 4200 (Agilent). 500ng of DNase-treated total RNA was used to generate QuantSeq 3' mRNA FWD (Lexogen).