GSM8048869: e16_wildtp_rbh3k4m3_bc741; Mus musculus; OTHER
E16 wt, hGFAP-Cre; Smofl/fl (smo cko) cortices (n=3 each group) were dissected, and cells were isolated with the Accumax (Sigma-Aldrich A7089) following the manufacturer's instructions. For CUT&RUN, H3K27ac, H3K4me3, H3K27me3, and IgG antibodies were used. 10,000 cells were used for each experiment. For CUT&RUN, DNA libraries were constructed using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645, E7103) with index primers (NEB, E7645-S).