RNA-Seq of KO_LPSWGP_SYKi
The isolated RNA was extracted from BMDM of each condition as described. The reverse transcriptase and primers were performing 1st cDNA then followed by using DNA Polymerase I and RNase H to 2nd cDNA which have a single 'A' base and subsequent ligation of the adapter. The products are then purified and enriched with PCR amplification. We then quantified the PCR yield by Qubit and pooled samples together to make a single strand DNA circle (ssDNA circle), which gave the final library.