GSM8269128: PC9 EF1a-NKX2-1-dTag 2hr-dTagV-1NEG Rep1; Homo sapiens; ATAC-seq
ATAC-seq samples were prepared according to the Omni-ATAC protocol (Corces et al., 2017). Cells were tagmented using Illumina Tn5 enzyme, purified using a Zymo DNA Clean and Concentrator-5 Kit. ATAC-seq gDNA samples were PCR amplified for 5 cycles using NEBNext Ultra II Master Mix. Additional amplification was determined using qPCR on a QStudio 6 FLX real-Time PCR System, with the only modification being inclusion of ROX passive dye for qPCR. Samples were amplified for 2-3 additional cycles, column purified again using a post-ATAC-seq Zymo DNA Clean and Concentrator-5 Kit. Libraries were quantified by qPCR or Qubit and pooled. ATAC-seq libraries were sequenced by Broad Walk-Up Sequencing on a Nextseq 500 using 2x40bp sequencing.