GSM8003017: IL-10R KO CD45.2; Mus musculus; RNA-Seq
single cells were isolated with collagenase D from colons of chimeric mice engrafted with either IL-10R KO or IL-10R/CerS2 DKO Cd45.2 donors. Single cells were sorted with a BD Aria FACS sorter for CD11b/C11c/CD64+ cells. Sorted cells were processed with 10x Chromium Controller scRNAseq libraries were generated using the Chromium Next GEM Single Cell 3' v3.1 Library & Gel Bead Kit (10X Genomics) according to the manufacturer's protocol. Briefly, emulsions were generated using the 10X Chromium Controller (10X Genomics) for a targeted recover 10,000 cells. Barcoded cDNAs were isolated from the emulsion and amplified by PCR (11 cycles). The cDNAs underwent fragmentation, end repair, and A-tailing before addition of sample indexes by PCR (16 cycles).