GSM8178209: WT1 LP Second; Mus musculus; RNA-Seq
RNA was harvested using Rneasy mini kit (QIAGEN). RNA was then quantified using with Nanodrop and submitted for RNA-sequencing. Library preparation and whole transcriptome profiling was performed by The Francis Crick Institute Advanced sequencing facility. Sequencing libraries were prepared with 100ng total RNA with RN integrity (RIN) values of more than 7. The Nugen Ovation ultralow kit was used to prepare the sequencing libraries following manufacturer's instructions. Libraries were barcoded and run on an Illumina HiSeq 2500system with paired-end read lengths of 101 bp