GSM7090084: GenolabM_A1-2; Homo sapiens; OTHER
Three tumor FFPE samples obtained from patients with high-grade serous ovarian cancer were analyzed. Patients were treated in the Department of Gynecological Oncology, Cancer Research Institute of Tomsk National Research Medical Center (Tomsk, Russia). The study was carried out according to Declaration of Helsinki (from 1964, revised in 1975 and 1983) and was approved by the local committee of Medical Ethics of Tomsk Cancer Research Institute; all patients signed informed consent for the study. Shortly, FFPE tissue sections were placed on the Capture Areas of 10x Genomics Visium slides, deparaffinized, and stained by hematoxylin and eosin. Tumor tissue slides were scanned using the Leica Aperio AT2 station (Leica, Germany) and images were processed with Aperio ScanScope. Libraries were prepared using standard protocol provided by 10x Genomics (User Guide | CG000407). The obtained cDNA libraries were analyzed following 10x Genomics sequencing recommendation for FFPE Visium libraries. Sequencing cycles comprised 28 bp (Read 1), 10 bp (Index 1 and 2) and 50 bp (Read 2). Illumina NextSeq 2000 sequencing was performed using NextSeq 1000/2000 P2 reagents (100 cycles), whereas GeneMind Genolab M used FCM reagents (150 cycles).