GSM8179769: PBMC RNA, repeat, Low Blood pressure with Low LVMI , 10; Homo sapiens; RNA-Seq
Peripheral Blood samples (2.5 ml) were drawn from each volunteer in a PAXgene tube (Qiagen cat# 762165) followed by total RNA isolation using an RNA isolation kit (Qiagen cat# 763134) according to the manufacturer instruction. Total RNA concentration and quality were evaluated using a nanodrop system (ThermoFisher Scientific). After Sample QC was by qubit and Agilent Bioanalyzer, the Illumina TruSeq Stranded mRNA Library Prep kit (Illumina, San Diego, CA) was used, the mRNA in the total RNA was converted into a library of template molecules of known strand origin. Specifically, mRNA molecules were isolated using poly-T oligo attached magnetic beads. The resulting mRNA was fragmented and reverse transcribed using random primers. The cDNA product was then amplified, incorporating sequencing adapters and barcodes to create a final double-stranded cDNA library ready for sequencing. The samples were sequenced on the Illumina HiSeq platform rendering 50 bp single-end reads.