GSM6591769: Nov16Batch, 16p_Del, A12, NSC; Homo sapiens; RNA-Seq
All RNA samples were extracted with Trizol reagent according to the manufacturer's instruction (Invitrogen). Mouse and human cell RNASeq libraries were prepared with customized version of strand specific dUTP method and TruSeq Stranded mRNA Library Kit (Illumina); scRNA-seq libraries were prepared using the Chromium Single Cell 3' Library & Gel Bead Kit v3 (PN-1000075) according to manufacturer's instructions (CG000183) using a Chromium Single Cell B Chip (PN-1000073) for a targeted cell recovery of 5,000 cells. RNAseq for mouse tissues and human NSCs and iNs; single-cell RNAseq for cerebral organoids