Small RNA Seq of rat aortic endothelial cells: Ox-LDL treatment 1
The 3 'end of RNA was connected to the connector with T4 RNA ligase 2. The 5 'end of RNA was connected to the connector with T4 RNA ligase 1. The connecting product was reverse transcribed to obtain the cDNA strand of the connecting product. In this step, the small RNA library was reverse transcribed into DNA. The reverse transcription product was amplified by PCR to obtain the final library product.