RNA-Seq of Pseudomonas brassicacearum CDVNBN10 cells detached from Brassica napus roots (gnobiotic plants grown on petri dishes innoculated with the bacterial strain)
In order to enrich the mRNA, we carried out a process of prokaryotic and eukaryotic ribodepletion with the Ribo-Zero kit (Illumina). The enriched mRNA was converted into cDNA, which was used to build a library with the TruSeq Stranded total RNA kit.