GSM7898195: multiplexed samples (tt2iLGo/hiTSC; DMSO/dm-aKG), GEX; Homo sapiens; RNA-Seq
Four samples were collected by 10 minutes of trypsinization and dissociated into single cells. Each sample was incubated with 0.5 μg of unique hashtag antibody for 20 min on ice. TotalSeq hashtag antibodies (A0253-A0256, Biolegend) were used to multiplex the samples (DOI: 10.1126/sciadv.adg4055). All 4 samples were sorted on a BD FACSymphony S6 (BD Bioscences) resulting in a total cell recovery of around 8000 cells, which were loaded onto a Chromium Next GEM chip (10x Genomics). Library preparation was performed according to the Chromium Next GEM Single Cell 3' v3.1 user guide with the addition of the hashtag library for demultiplexing. Combined libraries were sequenced using the NextSeq2000 P2-100 kit (Illumina) with Paired-end sequencing 10x scRNA-seq