RNAseq of Oophaga sylvatica: Cristobal Colon
Trizol RNA extraction, PolyA purification (New England Biolabs #E7490L), directional RNA sequencing kit (NEB #E7765L) with unique dual index barcodes (NEB #E6440S). Post-library prep quantification by Qubit and pooling in equimolar amounts within tissue.