GSM6774420: WT_CD, WT_ID, APT_CD, APT_ID; Mus musculus; RNA-Seq
Freshly collected mouse hippocampus was dissociated with Adult Brain Dissociation Kit (Miltenyi) using manufacturer's protocols (37C_ABDK_02). Dissociated cells were stained with Fixable Viability Dye eFluorTM 660 (BD) for 30min, 4C for FACS sorting. FACS sorted cells were suspended with 0.04% BSA/PBS solution. Libraries were generated with the manufacturer's instructions (single cell 3' v3.1 protocol with CellPlex, 10x Genomics).