RNA-seq of Eurosta solidaginis: multiple stage sampling
Total RNA was extracted using the Zymo Research Direct-zol-96 MagBead RNA kit. Following RNA isolation, the NEBNext Poly(A) mRNA Magnetic Isolation Module protocol was used for poly A selection. NEBNext Ultra II Directional RNA Library Prep Kit was used to prepare RNA-seq libraries targeting a final library size of 300-600 bp. The resulting RNA-Seq library was sequenced as part of a barcoded pool on one 2 x 150 sequencing kit for the Element Biosciences Aviti system.