GSM7386082: YAM6_xrn1_Gs_1; Saccharomyces cerevisiae; RNA-Seq
Total RNA was extracted using standard hot phenol procedure. Extracted RNA was ethanol-precipitated, resuspended in nuclease-free H2O (Ambion) and quantified using a NanoDrop 2000c spectrophotometer and/or a Qubit fluorometer with the Qubit RNA HS Assay kit (Life Technologies). For each sample, 1 µg of total RNA was mixed with 2 µl of diluted ERCC RNA spike-in mix (1:100 dilution in nuclease-free H2O; Invitrogen). Ribosomal (r)RNAs were depleted using the Ribominus Eukaryote v2 kit (Ambion). Libraries were prepared using the TruSeq Stranded mRNA Sample Preparation Kit (Illumina) and the IDT for Illumina – TruSeq RNA UD indexes (Illumina).