GSM6621031: Acidosis 2; Homo sapiens; RNA-Seq
Total RNA from CCA cells was extracted using RNeasy Mini Kit (Qiagen). mRNA was purified using NEB Next Poly (A) mRNA Magnetic Isolation Module (NEB). cDNA library for sequencing was synthesized using NEB Next Ultra DNA Library Prep Kit for Illumina (NEB) and was sequenced on NextSeq 500 sequencer (Illumina) with 75-bps single-end reads.