GSM7393237: iwat, ko_1week, 2 [H1-KO2_S15]; Mus musculus; RNA-Seq
Total RNA was extracted using TRIzol and RNeasy Mini Kit with on-column DNase I digestion (Qiagen) Library preparation and sequencing was performed at the UCLA Technology Center for Genomics & Bioinformatics. Illumina sequencing-compatible cDNA libraries were synthesized from mRNA  enriched by Poly-A capture. 200bp paired-end sequencing cDNA libraries were analyzed with an Agilent TapeStation and sequenced on an Illumina NovaSeq S1 at 200 cycles. Read depth per sample averaged around 130M reads. Raw sequencing reads were demultiplexed using bcl2fastq (Illumina).