GSM8174003: CD45+ of Control tumor mice 1; Mus musculus; RNA-Seq
2 days after RT, mice were euthanized, and tumor tissues collected. Single-cell suspensions were prepared and CD45+ cells were positively selected using CD45 MicroBeads and viability (at least 85% of cell viability and >90% of CD45+ cell) was checked before encapsulation. Cells were encapsulated using the 10x Chromium Controller system and a Chromium Next GEM Single Cell 3ʹ Dual Index Kit v3.1. Reverse transcription and libraries were prepared according to the manufacturer's instruction.