GSM8048832: e15wt_gli3_yz052; Mus musculus; ChIP-Seq
E15 wild type cortices were dissected, fixed for 10 min with 1% formaldehyde and neutralized with glycine. The cells were lysed, and the chromatin was sheared into 100∼300 bp fragments. Immunoprecipitation reactions were performed using the Gli3 antibody For ChIP-seq, sequencing libraries were generated from the ChIP-ed DNA and input DNA for control using Ovation Ultralow System V2 kit according to the manufacturer's protocol.