ONT genomic sequencing of Aminobacter P9b grown on 0% D2O
Aminobacter P9b samples were incubated on a 0% D2O, 30% D2O and 100% D2O medium, respectively. Cells were harvested by centrifugation. Pellets were resuspended and DNA extracted. The DNA was barcoded using the ONTs native barcoding kit, prepared with the SQK-LSK109 protocol, and sequenced on a MinION with an r9 flowcell. Resulting reads were basecalled using Guppy v6.4.8 and the dna r9.4.1 450 bps sup model.