GSM8174483: La_m6A_rep2; Mus musculus; RIP-Seq
MuSCs were collected in 1 mL of Trizol and left on ice for 5 minutes. 200 μL of chloroform was added and mixed thoroughly. The cell samples were centrifuged at 12000 rpm for 10 minutes at 4°C. The supernatant was collected, and an equal volume of isopropanol was added, followed by incubation at -20°C for 2 hours. Finally, the RNA was collected by centrifugation at 12000 rpm for 10 minutes at 4°C, and the precipitate was washed with 80% ethanol, followed by dissolution in nuclease-free water. RNA was used to construct a sequence library using an RNA-seq Library Prep Kit (Vazyme, NR605), followed by sequencing analysis using Illumina HiSeq X10.