Bacterial 16S of roots from olive trees without pest
Metagenomic analyses of the V3-V4 region from bacterial 16S gene were performed on the primers 16S ProV3V4 forward (5' CCTACGGGNBGCASCAG 3') and 16S ProV3V4 reverse (5 GACTACNVGGGTATCTAATCC 3) . To prepare libraries for Illumina sequencing, primers 16S ProV3V4 were fused with the Illumina adapter overhang nucleotide sequences. For each library, root PCR products were sequenced on the Illumina MiSeq platform using a 2300 nucleotide paired-end protocol.