GSM8269959: BEAS-2B, ATAC, rep1; Homo sapiens; ATAC-seq
Approximately 1 million cells were harvested and lysed, then the extracted chromatin was treated with Tn5 transposase loaded with preloaded barcodes. Library amplification was conducted using NEBNext High-Fidelity 2x PCR Master Mix (New England Biolabs Cat #M0541), followed by size selection using a 0.7% agarose gel.