GSM7011852: Sample_1_T-cells, CD38+/CD39+; Homo sapiens; RNA-Seq
CD4+ cells were flow sorted into CD4+ CD127-GARP-CD38-CD39-, CD4+ CD127-GARP-CD38+CD39-, CD4+ CD127-GARP-CD38-CD39+, or CD4+ CD127-GARP-CD38+CD39+. Total RNA was isolated from the sorted cells with either a Qiagen RNAEasy Plus Micro kit or a Qiagen RNAEasy Mini kit. RNA integrity numbers (RIN) for each sample were determined for each sample with an Agilent 2100 Bioanalyzer. Only samples with a RIN ≥ 8.0 were used to prepare cDNA libraries with a CloneTech SMARTer HT kit. Sequencing was performed by the NYU Langone's Genome Technology Center (RRID: SCR_017929) on an Illumina NovaSeq 6000, generating approximately 50 million 100bp paired reads per sample.