GSM8178138: DN.mTEClo.DonorE; Homo sapiens; RNA-Seq
Tissue was minced with scissors and lymphocyte-rich supernatant was removed, then thymic fragments were digested with collagenase and dispase. EpCAM+ cells were magnetically enriched (Donors A, B, and E) or CD45+ cells were magnetically depleted (Donors C and D). Cells were stained with fluorescent antibodies and flow sorted into DMEM/25 mM HEPES/10% FCS. Single cells were encapsulated following 10x Genomics protocols. 10x Genomics library prep, 3' v3.1 chemistry