Whole exome sequencing of urothelial cancer
Pre-and post-treatment sequencing libraries from samples from the clinical trial: Phase 2 Study of Pembrolizumab in Combination With Gemcitabine and Cisplatin as Neoadjuvant Therapy (NCT02690558) were prepared from 100 ng of DNA using the Illumina DNA Prep with Enrichment kit and the Illumina Exome Panel. During library preparation, DNA was amplified with IDT for Illumina DNA Unique Dual Indexes, resulting in a pool of twelve dual-indexed paired-end libraries