NextSeq 550 paired end sequencing; Host transcriptome determinants for African Swine Fever (ASF)
Host transcriptome determinants for African Swine Fever (ASF)
Peripheral blood mononuclear cells (PBMC) were prepared from heparinised blood using Histopaque and frozen in foetal calf serum supplemented with 10% DMSO. Cells were defrosted into warm RPMI GlutaMAX HEPES media supplemented with 10% foetal calf serum, 1 mM sodium pyruvate, 50 µM 2-mercaptoethanol, 100 IU/mL penicillin and 100 µg/mL streptomycin (RPMI/10). Nucleic acid was extracted from one million cells using a MagMAX mirvRNA Total RNA extraction kit on a MagMAX™ Express-96 Deep Well Magnetic Particle Processor. 3'UTR sequencing