GSM6509959: piwi-2(RNAi) biol rep 1; Schmidtea mediterranea; RNA-Seq
4 animals per replicate were homogenized in 200 ul TEN buffer (0.1 M Tris-Cl pH 8.0, 0.01 M EDTA pH 8.0, 1 M NaCl) supplemented with SDS (0.4%), proteinase K (0.48 mg/ml) and beta-mercaptoethanol (0.14%) and pipetted gently with a multichannel pipette every 2-3 minutes at room temperature until homogenized (~10 minutes), then RNA extracted using 600 ul Trizol-LS reagent. Kapa stranded mRNA seq, 96-well format