Whole Genome Sequencing of Penicillium citrinum B9
A sequence of nucleotides incorporated by the DNA polymerase while reading a template, suchas a circular SMRTbell (TM) template. Polymerase reads are most useful for quality control of theinstrument run. Polymerase read metrics primarily reflect movie length and other run parametersrather than insert size distribution. Polymerase reads are trimmed to include only the high qualityregion; they include sequences from adapters; and can further include sequence from multiplepasses around a circular template. Each polymerase read is partitioned to form one or more subreads, which contain sequence froma single pass of a polymerase on a single strand of an insert within a SMRTbell (TM) template andno adapter sequences. The subreads contain the full set of quality values and kinetic measurements.Subreads are useful for de novo assembly.