GSM6592136: Omentum tumor 1191, position C; Homo sapiens; RNA-Seq
Formalin-fixed paraffin-embedded (FFPE) tumor tissue was sectioned onto a Visium Spatial Expression Slide (Serial Number: V10D07-67, 10X Chromium) and was visualized via H&E. RNA within the tissue was hybridized to barcoded probes. 10x genomics Visium library prep for FFPE human samples following the standard protocol. Each Visium Spatial Gene Expression Slide contains Capture Areas with gene expression spots that include primers required to capture the probes. Tissue sections placed on these Capture Areas are deparaffinized, stained, and decrosslinked, as described in Deparaffinization & Staining Demonstrated Protocols – CG000409 or CG000410. The RNA-probe complexes were amplified using KAPA qPCR and AMP (Cat. # 2000047)kits. Indexed primers are used from Dual Index Plate TS Set A (Cat. # 3000511). Visium FFPE probe