GSM8305415: R2_K562_replicate2; Homo sapiens; RNA-Seq
Total RNA was extracted from cells 48h after transfection using the Monarch total RNA miniprep kit (NEB T2010S). For DNA libraries, barcode region of plasmid was extracted via PCR. mRNA was isolated from total RNA extracts using the magnetic mRNA Isolation Kit (NEB S1550S) then reverse transcribed with the Maxima H Minus Reverse Transcriptase (Thermo EP0753) using a UMI-containing RT primer. Plasmid DNA was prepared with the same UMI-containing primer.