GSM6622735: Embryo424_4_T; Ovis aries; RNA-Seq
Total RNA was extracted from embryos using the RNAqueous-Micro Kit (Ambion, Austin, TX, USA). DNA removal and whole transcriptome amplification were performed using the REPLI-g WTA Single Cell Kit (Qiagen, Germantown, MD, USA). RNA libraries were prepared for sequencing using standard Illumina protocols.