GSM7017027: F4/80+ cells isolated from medium-content tumor, Day11 [Y22_M_TAM_1]; Mus musculus; RNA-Seq
RNA was harvested using TRIzol Reagent (Life Technologies), total amounts and integrity of RNA were assessed using the Agilent 5400. RNA libraries for RNA-seq were prepared using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® following manufacturer's protocols, the effective concentration of the library is higher than that of 2nM.