HiSeq X Ten paired end sequencing of SAMD00603468
The DNA fragments were sheared to 300 bp using a Covaris Focused-ultrasonicator S220 (Covaris). NGS libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (NEB) and NEBNext Multiplex Oligos for Illumina (NEB). These libraries were deep-sequenced by a commercial company (Macrogen) using HiSeq X (2 x 150 bp; Illumina).