GSM8174033: Anti-IgG Hepatocyte Organoids; Mus musculus; OTHER
The library preparation was performed with a CUT&Tag Assay Kit (Vazyme) by the manufacturer's instructions. To amplify libraries, 15μL DNA was mixed with 5μL of a universal i5 and 5μL of auniquely barcoded i7 primer. A volume of 25 μL  2× CAM was added and mixed. The sample was placed in a Thermocycler with a heated lid using the following cycling conditions: 72 °C for 3 min (gap filling); 95 °C for 3 min; 9~20 cycles of 98 °C for 10 s and 60 °C for 5 s; final extension at 72 °C for 1 min and hold at 4 °C. libraries clean-up was performed VAHTS DNA Clean beads.