RNA-Seq of sorghum bicolor: BTx623 chilling PM
Total RNA was extracted from leaf tissue using the RNeasy Plant Mini Kit (Qiagen, USA). The Spike-in RNA Variant Controls (SIRVs) Set 3 (Lexogen, USA) were added after tissue grinding to obtain 90 pg of SIRV per leaf for RNA spike-in normalization. For library preparation, 300 ng of total RNA was used with the QuantSeq 3'mRNA-Seq Library Prep Kit for Illumina (Lexogen, USA). The libraries were sequenced using the Illumina NovaSeq 6000 platform with 100-bp single reads.