Whole genome sequencing for de novo genome assembly of Arctocephalus gazella: skin tissue sample
To prepare the extracted DNA for the Omni-C libraries, chromatin was fixed in place in the nucleus with formaldehyde. The chromatin was then extracted and digested with DNase I and chromatin ends were repaired and ligated to a biotinylated bridge adapter. Then the ends containing adapters were proximity ligated and crosslinks were reversed. Afterwards, the DNA was purified and biotin that was not internal to ligated fragments was removed. Using NEBNext Ultra enzymes and Illumina-compatible adapters, the sequencing libraries were then generated. Before PCR enrichment, biotin-containing fragments were isolated with streptavidin beads for each library