Venom Gland RNA-seq from Crotalus atrox
A NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, MA, U.S.A) was used to select mRNA from 1 g of total RNA, and the NEBNext Ultra RNA library prep kit (New England Biolabs, MA, U.S.A) manufactures protocol followed to prepare the sample for Illumina RNA-sequencing (RNA-seq). During library preparation, products within the 200-400 bp size range were selected by solid phase reversible immobilization with the Agencourt AMPure XP reagent (Beckman Coulter, C.A., U.S.A.) and PCR amplification consisted of 12 cycles.