ChIP-seq of S. cerevisiae: EtOH 1hr treated in Spt5p-AID, pSer2 IP
ChIP was conducted as reported (Strahl-Bolsinger et al., 1997) with minor modifications. The extracted DNA was purified using a QIAquick PCR purification kit (Qiagen, 28106). Libraries for RNAPII ChIP-seq were generated using the NEXTflex Illumina ChIP-Seq Library Prep kit (BIOO, 5143-02), following the manufacturers protocols.