GSM7659924: ERRBS_4month_tet2_mut_rep1; Danio rerio; Bisulfite-Seq
DNA was extracted  from marrow cells using Qiagen's allprep extraction kit per manufacturer's recommendation ERRBS was performed as previously described. Briefly, high molecular weight DNA isolated from the progenitor cells was digested with the MspI restriction enzyme. Fragments were purified and subjected to end repair, A-tailing and ligation of methylated Illumina adapters. This was followed by bisulfite conversion and PCR amplification. Libraries were sequenced on an Illumina 2500 using a 50bp single end read approach per manufacturer's recommendations.