Environmental DNA from Lake Pounui sediment core
Extraction of environmental DNA from different depths of lake sediment core from Lake Pounui in NZ. About 10g of sediment was extracted using a custom alkaline butter extraction followed by the Dneasy Powersoil Kit. PCRs were undertaken using 12S rRNA primers. PCRs were combined, cleaned and had a second round of PCR to attach sequencing indexes. Sequencing was undertaken on an Illumina Nova Seq