GSM8178144: Control Rep 6; Rattus norvegicus; RNA-Seq
RNA was isolated using Qiagen's RNeasy Plus Universal (Qiagen, ID 73404) according to manufacturer's instructions. All RNA samples were quantified using a NanoDrop (Thermo Fisher Scientific), and quality assessed using an Agilent 2100 Bioanalyzer. All RNA samples had an RNA Integrity Number (RIN) ≥9. The library preparation was completed using the QIAseq Stranded mRNA Select Kit (Qiagen) utilizing 500 ng of RNA as input. Library quality controlled was performed using an Agilent 2100 Bioanalyzer. Libraries were pooled in equimolar concentrations, and pools quantified by qRT-PCR before sequencing on an Illumina NovaSeq according to the manufacturer instructions.