GSM6505555: THP-1 cells, RNAseq/LPSDex, 3h, rep3; Homo sapiens; RNA-Seq
Total RNA was isolated using TRIzol Reagent (Invitrogen) using 3M of the exact same human THP-1 cells processed for ribosome profiling. RIN scores were measured on a BioAnalyzer 2100 using the RNA 6000 Nano assay (Agilent). Poly(A)-purified mRNA-seq libraries were generated from high quality RNA (average RNA Integrity Number (RIN)) of 7.7. RNA-seq library preparation was performed according to the TruSeq Stranded mRNA Reference Guide, using 500ng of total RNA as input. Libraries were multiplexed and sequenced on an Illumina NovaSeq 6000 producing paired 2x101nt reads. THP-1 mRNA-seq libraries were sequenced to an average depth of 160M (min. 81M, max. 207M) raw reads. RNA-seq library was prepared according to the protocol as described in TruSeq Stranded mRNA Reference Guide.