GSM8084341: CTR (dimethylsulfoxide), biological replicate 1; Homo sapiens; miRNA-Seq
Isolation of small RNAs from PBMCs was performed using the total exosome RNA and protein isolation kit (Thermo Fisher Scientific). To construct the libraries, 12 EVs isolated from independent PBMC cultures each grown in untreated (DMSO) and 2 µM B[a]P-treated conditions for 48 h, were combined in 3 equal pools (with 4 separate blood donors per pool) for each condition. 5 µL of each pool were used to isolate the small RNA fraction and processed using Qiaseq miRNA library prep kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions.