GSM7180360: SPF185, anti-FLAG antibody, rep1; Aspergillus fumigatus; ChIP-Seq
Cross linked mycelia were harvested and then ground after freezing in liquid nitrogen. Samples were hydrated in ChIP lysis buffer (CLB: 50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% Deoxycholate (Sigma D6750), 0.1% SDS, 1 mM PMSF, 1× fungal proteinase inhibitor cocktail (Sigma)), then sheared using Covaris sonication. Sheared chromatin was precipitated withanti-FfmA or anti-FLAG (Sigma)antibodies and recovered using magnetic beads. Cross links were reversed and DNA recovered for library preparation. Libraries were generated using the Rubicon ThruPlex DNAseq kit as described by the supplier.