oral metagenome
Shotgun metagenomic sequence libraries were constructed from the DNA extracts using Illumina Nextera XT Flex kits according to manufacturer recommendations and then sequenced on an Illumina HiSeq 4000 platform (150 bp paired-end mode) at the Genomic Resource Center at the University of Maryland School of Medicine. Each sample was uniquely barcoded in each HiSeq 4000 lane, yielding an average of 40 million read pairs for each sample.