SLAF of Tetrastigma hemsleyanum
Total genomic DNA was extracted from the silica-dried leaf tissue using DNA Plantzol (Invitrogen) following the manufacturers protocol. Specific length amplified fragment (SLAF) sequencing libraries were prepared using two restriction enzymes RsaI and HaeIII. Specific single nucleotide A overhangs were added to the 3 end of the resulting fragments and connected to dual-index sequencing adaptors. The resulting digested fragments ranging from 314 to 414 base pairs (with barcodes and dual-index sequencing adaptors) in size were excised and purified using a Gel Extraction Kit (Qiagen). After the library quality inspection, paired-end sequencing with read lengths of 126 bp was performed using an Illumina HiSeq 2500 system (Illumina, Inc., San Diego, CA, United States) according to the manufacturers recommendations.