16S rRNA amplicon sequencing of Gyrinophilus porphyriticus skin microbiome
We used a two-step PCR library prep and dual-index paired-end Illumina sequencing to sequence the skin microbiome of each salamander skin swab sample, plus positive and negative controls. We amplified the V3-V5 region of the 16S rRNA gene (~380 bp) using the universal primers 515F-Y and 939R.