GSM8268678: NSC, wt, RNA, rep1; Homo sapiens; RNA-Seq
RNA was isolated from two independent cultures following TRI reagent (Sigma) manufacturer's instruction. RNA was further purified using column purification (Qiagen, #74204). mRNA capture, library prep and barcoding were performed according to standard procedures of the Erasmus MC Biomics facility. Libraries were then single end sequenced, 50bp, on an Illumina HiSeq2500 machine at a depth of approximately 20 million reads per sample.