GSM7871146: SH-SY5Y cell line, shDDR2, 24h, 2kPa, bioreplicate 3; Homo sapiens; RNA-Seq
RNA was extracted using the RNeasy microKit (QIAGEN) combined with DNase (TurboDNase (ThermoFisher). The following RNA concentration and integrity were measured by the Qubit 4 Fluorometer (Thermo Fisher Scientific) and 2100 Bioanalyzer (Agilent), respectively. We used the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® kit to make all RNA libraries without any modifications. For each library, the input of approximately 500 ng total RNA, and a total of nine PCR cycles were used for library amplification. The quality and concentration of libraries were measured by 2100 Bioanalyzer (Agilent) and Qubit 4 Fluorometer (Thermo Fisher Scientific), respectively. All libraries were normalized to 20 nM and pooled together, the final concentration of library pool was 5 nM, loaded onto the NextSeq500/550 High Output Kit v2.5(150 Cycles) chip (Illumina), and sequenced on the NextSeq550 (Illumina).