16S rRNA gene amplicons collected from soils from the Gaughan Farm, Southwest Ontario, CA
Total genomic DNA was extracted from duplicate soil sub-samples (2 x 0.25 g each) using the Qiagen PowerSoil high throughput DNA Isolation kits (Qiagen Inc., Canada). After quality assessment, DNA was normalized to 10 ng/?L and shipped to Genome Quebec for high-throughput 16S rRNA gene amplicon sequencing (515F-Y/926R) on a NovaSeq6000 (SP, 2 x 250 bp) platform.