Illumina NovaSeq 6000 paired end sequencing; RNAsequencing from mouse E11 WT and Gata2 heterozygous Pre1-, Pre2- and pro-HSCs
RNAsequencing from mouse E11 WT and Gata2 heterozygous Pre1-, Pre2- and pro-HSCs
AGMs were microdissected and cells were dissociated using collagenase III. Cells were sorted using a FACS Aria III in trizol RNA was extrated from trizol cDNA was smarter amplified