GSM8305745: LNCaP cell, Ctrl; Homo sapiens; RNA-Seq
Cells were washed with PBS 1x and detached by scraping in PBS/5mM EDTA/sodium vanadate, pelleted, and re-suspended in TRIzol for RNA extraction Sample quality control wasperformed using the Agilent 2100 Bioanalyzer or the Agilent 4200 Tapestation. Qualifying samples were then prepped following the standard protocol for the Illumina Stranded mRNA prep (Illumina). Sequencing was performed on the Illumina NextSeq2000 with Paired End 59bp × 59bp reads.