GSM8388870: Adult hermaphrodite 24hr+midL4, prg-1(∆PAZ-domain), rep 1; Caenorhabditis elegans; miRNA-Seq
Small RNAs were extracted using a mirVana miRNA isolation kit (Ambion) following manufacturers protocol. Purified small RNA samples were treated with Acid Pyrophosphotase to allow for cloning of small RNAs bearing a 5' triphosphate. Libraries were contructured using a TruSeq Small RNA Library Preparation Kit (Illumina).