GSM6896342: ECs_Mac_Depleted_Replicate_3; Mus musculus; RNA-Seq
ECs were FACS-sorted from digested, LPS-stimulated colon mucosa with and without macrophage depletion by gating on CD45-/Live1-/CD31+ cells. Total mRNA was extracted from sorted ECs using RNeasy Plus Mini Kit (Qiagen), followed by quantification with Qubit and RIN quality assessment by 2100 Agilent Bioanalyzer (Agilent Technologies, Santa Clara. CA). Low-input total cDNA library construction and Illumina sequencing: RNA-seq was conducted at the NUSeq Core Facility using a Illumina HiSeq 4000 NGS System (Illumina, San Diego, CA) to generate 50 bp reads with 300 million paired-end reads and 25 million reads per sample.