GSM6429994: RNA-Seq th17 replicate 1; Mus musculus; RNA-Seq
Total RNA was isolated by using the RNeasy kit (Qiagen) while the concentrations and integrity were quantified by Bioanalyzer (Agilent); and the polyadenylated mRNAs were captured with PolyT primers, the Smart-seq2 reverse transcription reactions were performed. RNA libraries for RNA-seq were preAfter pre-amplification and AMPure XP bead purification, cDNAs were sheared with Covaris and were subject to Illumina TruSeq library preparation. All libraries were sequenced on Illumina HiSeq X Ten according to the manufacturer's instructions.