GSM7396142: H3K27ac_HiChIP.OIS.Rep1.Batch2; Homo sapiens; OTHER
The HiChIP experiments were carried out following the protocol previously described (Mumbach et al, Nature Methods, 2016), using the H3K27ac antibody (Abcam, ab4729). Briefly, 10 million cells were crosslinked using 1% formaldehyde for 10 minutes, and then sonicated for 6 minutes. After the immunoprecipitation step, DNA were purified and then quantified using Qubit. The amount of Tn5 used and the number of PCR cycles were determined as previously described based on Qubit quantification. After PCR amplification, size selection was performed using Ampure beads. Libraries were sequenced in paired-end mode on a NextSeq 550 instrument.