CRISPR/Cas9 mutation readout on the pathway reporters in hTERT RPE-1 p53KO or p53/BRCA1dKO on sample: hTERT_RPE1_Cas9_p53_BRCA1_dKO_DSBTRIP_manual_DMSO_72h_R1_T2_Run1
CRISPR/Cas9 mutation readout on the pathway reporters in hTERT RPE-1 p53KO or p53/BRCA1dKO after ATM inhibitor treatment. Five days after knock-out induction, a second gRNA is transfected to induce DSB in pathway reporters in parallel. Three days later, cells are harvested for mutation readout PCR.