KF19Q120S_3
Genomic DNA was extracted from a total of rhizosphere samples derived from 300 genotypes at two field locations using the E.Z.N.A. soil DNA Kit (Omega Bio-tek, Norcross, GA, U.S.) according to the manufacturers protocol. All DNA samples were quality checked and the concentration was quantified by NanoDrop 2000 spectrophotometers (Thermo Fisher Scientific, Wilmington, DE, USA). Bacterial 16S rRNA gene fragments (V3-V4) were amplified using primers 338F (5'-ACTCCTACGGGAGGCAGCAG-3') and 806R(5'-GGACTACHVGGGTWTCTAAT-3') at the following PCR conditions: 30 s at 95 C, 30 s at 55 C, and 45 s at 72 C for 27 cycles. PCRs were performed with 4 L 5  TransStart FastPfu buffer, 2 L 2.5 mM deoxynucleoside triphosphates (dNTPs), 0.8 L of each primer (5 M), 0.4 L TransStart FastPfu DNA Polymerase, 10 ng of extracted DNA, and finally using ddH2O to make up 20 L. Agarose gel electrophoresis was performed to verify the size of amplicons. Amplicons were subjected to paired-end sequencing on the Illumina MiSeq sequencing platform using PE300 strategy.