GSM7802716: HEK293T transfected with dCasRx-RBM25 and MAPT-targeting gRNA, replicate 2; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNeasy Mini Kit (QIAGEN) with on-column DNase treatment as recommended by the manufacturer. 500ng of total RNA was used for generation of polyA+ libraries with the NEB Next Ultra II Directional kit. Sequencing was performed by the Donnelly Sequencing Centre using a Nova-Seq 6000 instrument and S1 flow cell to an average depth of 50 million paired-end 150nt reads.