microsporidian diversity in atlantic ocean (coastal)
DNA was extracted using phenol-chloroform. Amplification of the eukaryotic V4 region was performed using the universal primer 515F and the eukaryotic primer 951R. The amplicons were purified and concentrated using the QIAquick Gel Extraction kit. See Chauvet et al. 2023 for the detailed protocol. In this article, data from both size fractions (or dates for Roscoff) were grouped, leading to only one sample per site.