GSM8269969: B2B, pol II, ChIP, rep1; Homo sapiens; ChIP-Seq
Collected cells were crosslinked with 1% formaldehyde for 10 minutes at room temperature. Quenching was done for 5 minutes using 125mM glycine, followed by two washes with cold DPBS. Chromatin was sonicated using a Qsonica Q800SR2 sonicator. Libraries were prepared using KAPA HyperPrep Kits following instructions.