GSM7011990: RNAseq_PF-502_rep2; Homo sapiens; RNA-Seq
The HUVEC cells were seeded in 6-well plate about 105 cells per well and treated the following day with either vehicle(DMSO) or PF-502(0.15μM) for 12hrs. And then, the cells were harvested to extract total RNA and reverse transcribed into cDNA(operate as described above). Three independent experiments were performed. RNA-seq analysis then was performed. The NEBNext® Ultra™ RNA Library Prep Kit for Illumina® was used to prepare the RNA-seq libraries. RNA-seq libraries were constructed using an Illumina stranded mRNA sample preparation kit (NEB, E7770) according to the manufacturer's protocol.