GSM6622797: HOPE1h-1; Rattus norvegicus; RNA-Seq
RNA was havested using Rneasy mini plus kit(Qiagen). 1.3ug of total RNA was used for the construction of sequence libraries. RNA libraries wered prepared using SMARTER mRNA-Seq library Prep Kit following manufacturers protocols  were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then PCR   amplification, the PCR product was purified by AMPure XP beads, and the library was   finally obtained.