GSM8173999: zebrafish embryo, 5dpf, wild type, rep 1; Danio rerio; RNA-Seq
Total RNA was extracted from WT and sas10zju2/zju2 mutant embryos at 5dpf using TRIpure Reagent (Aidlab, RN0102), with three independent biological repeats each, and was treated with DNase I (New England Biolabs, M0303S) to remove genomic DNA contamination. Total RNA for each sample was ligated with 3'RACE_adapter, followed by 3'RACE_adapter specific RT primer's reverse transcription. Sequencing libraries were derived from PCR amplification using forward primer containing zebrafish 5'ETS sequence and reverse primers (Illumina RPIs).