Sequencing of Canis lupus familiaris Saliva
According to the manufacturers instructions, DNA library preparation was performed using the Nextera XT DNA sample preparation kit (Illumina, San Diego, CA, USA). First, one ng input DNA from each sample was used for the library preparation, which underwent fragmentation, adapter ligation, and amplification. Then, Illumina libraries were pooled equimolarly, denatured, and diluted to a concentration of 1.5 pM. Next, DNA sequencing was performed on a MiSeq instrument (Illumina) using a 2X 250 bp Output sequencing Kit together with a deliberate spike-in of 1% PhiX control library.