GSM8178495: 4/74 time 0 replicate 2 [Barseq12]; Salmonella enterica subsp. enterica serovar Typhimurium str. ST4/74; Tn-Seq
At the end of the infection, THP-1 cells were lysed with 2% saponin for 30 minutes, and then DNA was isolated using the epicentre masterpure DNA extraction kit. Purified gDNA was subjected to a barcoding PCR using Q5 polymerase, with a universal P5 primer and unique P7 for each condition, according to published protocols in Wetmore et al., 2015 mbio. This PCR only captures the barcoded region of each Tn insertion.