GSM7659943: Exosome,DOR7; Homo sapiens; miRNA-Seq
Exosomal miRNAs were extracted using the miRNeasy Serum/Plasma Advanced Kit (#217204, Qiagen, Frederick, MD, USA) according to the manufacturer's protocol. For small RNA libraries, an input of 1 ng to 500 ng of RNA per sample was used for RNA sample preparations. Sequencing libraries were generated using the QIAseq miRNA Library Kit (Qiagen) following the manufacturer's recommendations, and index codes were added to assign sequences to each sample. Library quality was evaluated using the Agilent Bioanalyzer 2100 and qPCR. The index-coded samples were clustered using the acBot Cluster Generation System with the TruSeq PE Cluster Kitv3-cBot-HS (Illumina, San Diego, CA, USA) according to the manufacturer's instructions. After cluster generation, the library preparations were sequenced on an Illumina Hiseq platform, and paired-end reads were generated.