GSM7926951: RNA.cortex.ACSS2 KO.female.NQ5; Mus musculus; RNA-Seq
Briefly, total RNA was extracted using Trizol-chloroform. Total RNA quality was assessed on the Bioanalyzer platform using the RNA 6000 Nano assay (Agilent). mRNA was isolated from 300 ng total RNA using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (E7490L). Libraries were prepared using the NEBNext® UltraTM II RNA Library Prep Kit for Illumina® (E7770).