GSM7012039: Mouse_P0_D10_noFGF2-RNAseq_B1; Mus musculus; RNA-Seq
Briefly, 1-2 million in vitro cultured cells were washed with PBS and then harvested with trizol, and follow normal RNA extraction protocols. The RNA concentration and purifity were detected. RNA integrity was assessed by obtaining a RIN score >8 on the Agilent 2100 Bioanalyzer.  Then constructed the library with VAHTS TM Total RNA-seq (H/M/R) Library Prep Kit for Illumina kit (Vazyme, NR603). Libraries were prepared according to the manufacturer's protocol. The labeled samples were immediately amplified by 9–10 cycles of polymerase chain reaction (PCR) with barcoded primers and sequenced with a NovaSeq 6000 instrument in a 150 bp/150 bp paired-end run using standard Illumina protocols.