WXS of B-cell selected samples from individuals with CLL receiving BTKi treatment
Genomic DNA was sheared using a Covaris S2 Focused-ultrasonicator. DNA libraries were constructed using the Agilent Technologies SureSelect XT Low Input Reagent Kit (cat # G9703A). Pools of amplified libraries were enriched for exonic regions by hybridization with the Agilent SureSelect XT2 Human All Exon v7 probe set (cat# 5191-4008). Enriched libraries were qualified on an Agilent Technologies 4150 TapeStation using a D1000 ScreenTape assay (cat# 5067-5582 and 5067-5583).  The molarity of the libraries was defined by quantitative PCR using the Kapa Biosystems Kapa Library Quant Kit (cat#KK4824).  Libraries were normalized and sequenced on NovaSeq S2 Flow Cells with 2x150 bp sequencing per lane.