RNA-Seq of Trichoplusia ni: midgut control diet
Trichoplusia ni larvae were exposed to control or nicotine-containing (1% FW) McMorran diet for 24 h. Midgut and Malpighian tubule samples were dissected from 4th instar larvae following dietary exposure. RNA was extracted using Purezol and purified. Three samples for each tissue and treatment totalling 12 samples were sent to BGI Genomics for sequencing. RNA-seq was carried out on the BGISEQ-500 platform with 100 paired-end reads and an output of 30 million clean reads. Transcriptome reads were subjected to quality control and processed for differential gene expression analysis. RNA-seq data was validated by RT-qPCR.