GSM8269447: control diet, astrocyte ribotag, biol rep 1; Mus musculus; RNA-Seq
Mice were were euthanized with carbon dioxide, and brains were homogenized and incubated with anti-HA antibody. Magnetic IgG beads were used to isolate ribosome-bound mRNA. RNA was purified using the RNeasy Micro kit from Qiagen. Non-stranded mRNA libraries were made using Illumina's TruSeq RNA Library Prep Kit v2 according to manufacturer's instructions