GSM7179797: CD8 T cells, OT1-OVA-2-batch2; Mus musculus; RNA-Seq
Spleens and lymph nodes were pooled. OT-I and OT-II were sorted into Trizol using fluorescence activated cell sorting (FACS) and frozen at-80. RNA was extracted (Qiagen), and three mice were pooled per replicate. Pooled samples were converted to cDNA using SmartSeq protocol