Column 7 Port 3
total genomic DNA was extracted from triplicate 0.5 g subsamples using the MP FastDNA SPIN kit for soil (MP Biomedical, OH, USA) and then amplified using universal primers for Bacteria and Archaea (An, et al., 2013). The Applied Genomics Core at University of Alberta (Edmonton, Canada) performed a second round of amplification using Illumina bridge PCR-compatible primers followed by sequencing using an Illumina MiSeq platform (Illumina, San Diego, CA, USA). Quality-verified sequences were compared against the SILVA taxonomic database (r138.1; last Updated: 14.12.2021) and clustered into Operational Taxonomic Units (OTUs) at 3% distance.