GSM6814167: Str IP P-p38 R1; Drosophila melanogaster; ChIP-Seq
Lysates were clarified from sonicated nuclei and chromatin was immunoprecipitated with αP-p38 antibody. Finally DNA was purified by standard phenol extraction. 2-3 ng of DNA was used for library preparation. Endrepair, adenylation, ligation of adapters and PCR enrichment for 10 cycles was performed using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) according to manufacturer's recommendations. Purified libraries were quantified by Qubit dsDNA HS Assay Kit (Invitrogen) and size distribution was evaluated using Bioanalyzer DNA 1000 assay (Agilent).