GSM8305452: Lung_Niche_AML1, scRNA-seq; Mus musculus; RNA-Seq
Lungs were enzymatically digested with Liberase and DNaseI mixture, single-cell suspensions were sorted and  non-hematopoietic niche populations from all samples were isolated. For the AML-infiltrated lung samples, sorted AML/tumor cells (GFP+) were pooled with the niche cell suspensions (20% Tumor, 80% lung niche) and all  llibraries were generated using the Chromium™ Single Cell 3' Reagent Kits (v3.1, 10x Genomics). Single Cell 3' Reagent Kits (v3.1, 10x Genomics).