HA-CAR-T cells, donor 2
Approximately 100,000 CAR T cells were used per sample. Nuclei were isolated with ATAC-LB (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL) and used for tagmenta- tion using Nextera DNA Library Preparation Kit (Illumina) from three donors. After tag- mentation DNA was purified with MinElute PCR Purification Kit (Qiagen). Tagmented DNA was then amplified with Phusion high-fidelity PCR master mix (NEB) using 14 PCR cycles. Amplified libraries were purified again with MinElute PCR Purification Kit. Fragment distribution of libraries was assessed with Agilent Bioanalyzer and libraries were sequenced on the Illumina NovaSeq 6000 PE150 platform at a depth of 3  107 reads per sample. Sequencing was performed by Novogene (Sacramento, CA).