GSM6935062: mouse1_ATAC_s04; Mus musculus; ATAC-seq
Omni-ATAC-seq sample preparation was performed as previously described. After flow cytometric sorting, 50,000 cells were lysed with 50 µl cold ATAC-Resuspension buffer (RSB) (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% Tween 20) with 0.1% NP40, and 0.01% Digitonin on ice for 3 min. Lysis was stopped with 1 mL cold ATAC-RSB. After centrifugation (500×g for 10 min), nuclei were resuspended in 50μl transposition reaction containing 2.5μl Tn5 transposase (FC-121–1030; Illumina) to tag and fragmentalize accessible chromatin. The reaction was incubated at 37°C, 400rpm for 30 min; DNA was then purified using a MinElute kit (QIAGEN) and amplified with 8–12 cycles of PCR based on the amplification curve. Samples were purified using a MinElute kit, sequenced on a NovaSeq 6000, paired end, 50 cycles