GSM8384004: UV-RIP-seq_IgG_rep1; Homo sapiens; RIP-Seq
Total RNA was extracted from samples using TRIzol reagent (Invitrogen) according to the manufacturer's instructions. Total RNA-seq (ribo-depleted) libraries for total RNA-seq and UV-RIP-seq samples were generated and sequenced by Admera Health. Briefly, isolated RNA sample quality was assessed by High Sensitivity RNA Tapestation (Agilent Technologies Inc., California, USA) and quantified by Qubit 2.0 RNA HS assay (ThermoFisher, Massachusetts, USA). Libraries were constructed with KAPA™ RNA HyperPrep with RiboErase (Roche, Indiana, USA) and performed based on manufacturer's recommendations. Final library quantity was measured by KAPA SYBR® FAST qPCR and library quality evaluated by TapeStation D1000 ScreenTape (Agilent Technologies, CA, USA). The final library size was about 430 bp with an insert size of about 200bp. Illumina® 8-nt dual-indices were used.