GSM8003713: Strain B6-F_resc, CEHC1 rescued, rep 3; Chlamydomonas reinhardtii; RNA-Seq
Culture aliquots containing 3 x 10^7 cells were centrifuged to pellet the cells and lysis buffer was added to the cell pellet.  Processing of each cell sample was completed within 1.5 min.  RNA was isolated using the LiCl precipitation method (Wilkerson et al., 1994). RNA quality was assessed using the Agilent Bioloanalyzer; RIN value exceeded 8 for each sample.  RNA libraries were prepared for sequencing using standard Illumina protocolsRNA libraries were prepared for sequencing using standard Illumina protocols RNA-Seq HiSeq 2500, High Output Mode, Paired End Read, 125 bp