RNA-Seq of conidia and mycelial: Cordyceps strain
PCR products were sheared into 100150 base pair (bp) fragments, ligated to sequencing adapters, nick-translated, and amplified to yield a library for each individual. Gel-purified PCR products from each donor were sheared into 100150 bp fragments and ligated to barcoded sequencing adapters. The resulting products were nick translated and amplified three to five cycles to yield a library for each donor.