scRNA_CO1-173
Cells expanded in Defined Keratinocyte SFM (Gibco), Cell dissociation in Accutase or Accumax (Innovative cell technologies), washed in 1X PBS (calcium and magnesium-free) containing 0.05% weight by volume BSA. Only when required, dead cell removal stratagie was with (Miltenyi) MACS dead cell removal kit and then processed using the 10X Genomics Next GEM scRNA-Seq solution. Library construction followed the 10X Genomics Next GEM scRNA-Seq pipeline.