GSM6507336: Eosinophils, papain, cKO 1; Mus musculus; RNA-Seq
Eosinophils were sort-purified as Sytox blue- CD45+ Lineage (CD3, CD5, CD19, Ly6G)-CD11b+ Siglec F+ from the lung of Nmur1Cre-T2A-GFP Id2flox/flox and control mice on day 7 after papain administration. Cells were sorted into RLT Plus Lysis Buffer supplemented with 40 mM DTT and RNA was isolated using the RNeasy Plus Micro kit (Qiagen) according to the protocol provided by the manufacturer. RNA libraries for RNA-Seq were prepared using theSMARTer Stranded Total RNA-Seq Kit – Pico (Takara)