GSM8174646: NGP cells, STM, 6D, rep3; Homo sapiens; RNA-Seq
Total RNA was harvested from cells using the TRIzol reagent (Invitrogen). Poly(A) RNA was extracted using the Dynabeads mRNA DIRECT Purification kit following the manufacturer's protocol. RNA was purified by RNA Clean & Concentrator Kits (Zymo Research) and cDNA libraries were constructed using the SMART® cDNA Library Construction Kit (Takara) following the manufacturer's instructions.