16s
The bacterial DNA from the cecal contents (8 samples/group) was extracted as previously described. 1% agarose gel was used to determine the quality of DNA samples. After measuring the concentration, approximately 2030 ng of qualified DNA was used to prepare the library and subjected to Illumina sequencing performed by Meiji Biotechnology Co., Ltd (Shanghai, China). The V3 and V4 hypervariable microbial 16S rDNA regions were amplified by PCR using the following primers: 38F (5-ACTCCTACGGGAGGCAGCAG-3); 806R (5-