GSM6774564: 17q1qMYCN_D14_3; Homo sapiens; RNA-Seq
Total RNA was extracted using RNA Clean-Up and Concentration Kit (NORGEN BIOTEK CORP) following the instructions of the manufacturer The amount of total RNA was quantified using the Qubit 4.0 Fluorometric Quantitation system (Thermo Fisher Scientific) and the RNA integrity number (RIN) was determined using the 2100 Bioanalyzer instrument (Agilent). RNA-seq libraries were prepared with the QuantSeq 3'mRNA-Seq Library Prep Kit (FWD) for Illumina (Lexogen). Library concentrations were quantified with the Qubit 4.0 Fluorometric Quantitation system (Life Technologies) and the size distribution was assessed using the 2100 Bioanalyzer instrument (Agilent). For sequencing, samples were diluted and pooled into libraries in equimolar amounts. Libraries were sequenced by the Biomedical Sequencing Facility at CeMM using the Illumina HiSeq 4000 platform in 1x50bp single-end mode.