RNA-seq of Lotus japonicus Gifu WT Mock-inoculated solvent-treated
Root tissue from Mock-inoculated solvent-treated WT was harvested and snap-frozen in liquid nitrogen. RNA was extracted using Spectrum Plant Total RNA Kit (Sigma-Aldrich, USA). Isolated RNA was treated with DNase from TURBO DNA-free Kit (Thermo Scientific, USA) and verified for genomic DNA contamination by PCR using genomic-DNA specific primers. RNA samples were quantified with Nanodrop (Thermo Scientific, USA) followed by quality check with a Bioanalyzer (Agilent, USA) and quantification in Qubit 3.0 fluorometer (Invitrogen, USA). Samples with a RIN value of > 7 were used for library preparation using a QuantSeq 3` library kit (Lexogen, USA) and sequenced on Illumina HiSeq 2500 to obtain 100bp single end reads.