Illumina HiSeq 2500 paired end sequencing; RNA-seq of human post-mortem brain tissue of the frontal lobe from patients with FTD and healthy controls
RNA-seq of human post-mortem brain tissue of the frontal lobe from patients with FTD and healthy controls
The brain samples and/or bio samples were obtained from the Netherlands Brain Bank, Netherlands Institute for Neuroscience, Amsterdam (open access: www.brainbank.nl). All Material has been collected from donors for or from whom a written informed consent for a brain autopsy and the use of the material and clinical information for research purposes had been obtained by the NBB. Additional samples were provided by the Queen Square Brain Bank of Neurological Disorders and MRC, King College London. We complied to all relevant regulations of the mentioned brain banks for the work with these samples. Total RNA for RNA-seq was isolated from ±100mg of frozen brain tissue with TRIzol reagent (Thermo Fischer Scientific) according to the manufacturer recommendation, followed by purification with the RNeasy mini columns (Qiagen) after DNAse treatment.  Total RNA-seq libraries were prepared from 1 microgram of total RNA from frozen brain tissue using the TruSeq Stranded Total RNA with Ribo-Zero Gold kit (Illumina) according to the protocol specifications.