Genome sequencing of SARS-CoV-2
Viral RNA was extracted using the QIAamp Viral RNA Mini Kit. cDNA was synthesized by reverse transcription PCR (RT-PCR). cDNA was amplified by two separate PCR reactions using two distinct primer pools contained in the COVIDSeq PCR Master Mix 1 and COVIDSeq PCR Master Mix 2. Finally, the libraries were sequenced using a NextSeq2000 high-throughput reagent kit