GSM8306370: 7d_GEX; Mus musculus; OTHER
Lungs were harvested from control and β-glucan-treated mice and mechanically disrupted with scissors before being treated with Collagenase Type I, DNAse I and elastase (Worthington) for 30 minutes at 37°C. Cells were then passed through a 100 µm cell strainer and red blood cells were lysed. Single cell suspensions were stained with TotalSeq-tagged anti-CD45 and anti-Ly6G (Biolegend) according to the manufacturer's instructions. Single-cell GEXs and CSP libraries were generated using a Chromium Controller instrument (10x Genomics). Sequencing libraries were prepared using Chromium Single Cell 3′ Reagent Kits (10x Genomics), according to the manufacturer's instructions. GEX libraries were amplified with a thermal cycler for 13 cycles and CSP libraries for 10 cycles. paired scRNA-seq and CITE-seq