GSM8346276: SA_8h_5; Salmonella enterica subsp. enterica serovar Typhimurium str. ATCC 14028; RNA-Seq
The Salmonella were collected by centrifugation. Total RNA was extracted using RNA Extract Reagent (Evrogen, Russia) according to the manufacturer's protocol. DNA contaminants were removed using RNase-free DNase I kit (Ambion, USA). The integrity of the RNA was checked by Agilent 2100 bioanalyzer (USA). rRNA depletion performed using Ribo-Zero rRNA Removal Kit for Gram-Negative Bacteria (Illumina, USA). Barcoded RNA libraries were generated. NEBNext Ultra Directional RNA Library Prep Kit for Illumina was used to prepare RNA-seq libraries.