Corilla colletti from Balangoda, Ratnapura District, Sri Lanka (sample code: 9; extract no.: C38co)
RADseq libraries were prepared using a protocol in which sequenced regions were adjacent to single restriction-enzyme cut sites. Individual 250-ng DNA samples were digested with the restriction enzyme SbfI, uniquely barcoded and then pooled into multiplexed libraries. Each multiplexed library (50 l volume) was sheared ,and DNA was size-selected on an agarose gel. The multiplexed libraries were enriched and tagged with a second, unique (library-specific) barcode and were quality checked. Sequencing involved 250 bp, paired-end sequencing of each library on an Illumina MiSeq single-lane flow cell (MiSeq v 2 kit, 2 x 250 bp).