miRNA-Seq of Homo sapiens: Control O+ve RBCs with no malaria history
1) RNA Isolation protocol: Efficient isolation of small RNA-containing total RNA as well as simultaneous isolation and purification of small RNA (<200 nts; e.g., miRNA, pre-miRNA, tRNA, 5S RNA), large RNA (>200 nts; e.g., mRNA, 18S rRNA, 28S rRNA, pre-miRNA) was performed using the TAKARA Nucleospin manufacturer protocol. 2) Small RNA-seq library was prepared using the Lexogen-Small RNA-Seq Library Prep kit as per the manufacturers protocol. The library preparation includes 3adapter ligation, 5adaper ligation, reverse transcription of ligated RNA, Library amplification using PCR and purification before sequencing. 3) Sequencing of generated libraries by Novaseq 6000 using illumina platform using Lexogen kit