GSM6817118: FvRIF-cr-6_28DPA_Rep1; Fragaria vesca; RNA-Seq
Total RNA was extracted from fruits of 28 DPA WT fruits and 28 DPA FvRIF mutants (FvRIF-cr-6 and FvRIF-cr-13 at 28-day postanthesis using the E.Z.N.A. Total RNA Kit (Omega, Bienne, Switzerland). For library construction, 1 μg RNA was used for library construction with NEBNext ultra RNA library prepare kit for illumina (NEB, E7530). High-throughput sequencing was performed on the Illumina Nova seq 6000 sequencer with a paired-end read length of 150 bp according to the standard protocols.