GSM7978156: HIT-scISOseq Run4, scRNAseq; Mus musculus; RNA-Seq
Tthe same amplification procedure as SCAN-seq，except for changing the reverse transcription primer with a 10x gel bead for each reaction for the embryonic samples. Each pre-amplification product was purified by 0.6X Ampure XP beads (Beckman, Cat. A63882). The concentration was measured using Qubit™ dsDNA HS and BR Assay Kits (Invitrogen, Cat. Q32854). The PCR product from about 60 blastomeres which were confirmed of effective amplification were pooled together in proportion to the number of amplified cycles. We took 100ng of the pooled cDNA to build PacBio sequencing library following the protocol of HIT-scISOseq and sequenced for one cell with HiFi mode.