white maize
DNeasy PowerSoil Pro (The Scientific Group (Pty) Ltd. 2162 Gauteng, South Africa) was used to isolate DNA from maize rhizosphere samples as instructed. Library preparations utilized 50 ng of pure DNA from many sources. Enzyme processing damaged DNA. Repairing A-tailed DNA fragments. DNA with blunted or incompatible 5- and 3-ends is 5-phosphorylated. Klenow fragment adenylates blunted DNA segments' 3 ends and T4 polynucleotide kinase phosphorylates their 5 ends for TA ligation with Illumina adapters (Zhang et al., 2019). Library molecules are made by ligase enzymes covalently binding adaptors to DNA segments (Belton et al., 2012). Agilent 2100 Bioanalyzer measured library size after PCR. Pooling and diluting libraries to 0.6 nM gave Novaseq 6000 300 paired-end cycles.