GSM6813570: Liver, Control, HFD, rep2; Mus musculus; RNA-Seq
Total RNA was isolated using Trizol reagents (Thermo Fisher). cDNA libraries were prepared using an Illumina TruSeq RNA sample prep kit. The average size of the library cDNAs was 150 bp (excluding adapters). The integrity and quality of cDNA libraries were assessed using an Agilent 2100 Bioanalyzer and an ABI StepOne Plus real-time PCR system.