GSM6506001: 3_non-treated control cells; Mus musculus; RNA-Seq
Total RNA was isolated using QIAzol® (Qiagen, Valencia, CA, USA). Total RNA concentration was quantified using Quant-IT RiboGreen (Invitrogen, Waltham, MA, USA). The integrity of total RNA was determined using TapeStation RNA screentape (Agilent Technologies, Atlanta, GA, USA), and samples with RNA integrity numbers higher than 7 were used for library generation. A library was generated with 1 µg of total RNA from each sample using the Illumina TruSeq Stranded mRNA Sample Prep Kit (Illumina Inc., San Diego, CA, USA). First-strand cDNA was synthesized using SuperScript II reverse transcriptase (Invitrogen). Second-strand cDNA was synthesized using DNA polymerase I, RNase H, and dUTP. The cDNA samples were processed via end repair by adding a single “A” base and ligating the adapters. The library samples were analyzed using Illumina NovaSeq (Illumina Inc.).