GSM7394472: Rep3, ASO-L1+TPRX1-siRNA; Homo sapiens; RNA-Seq
RNA extraction was performed using the RNeasy mini kit (QIAGEN), according to the manufacturer's instructions. 32ng RNA each sample was used to prepare sequencing library. The cDNA library was prepared with NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #  E7490S) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB # E7760L), following the manual instruction. Library cDNA were equally pooled at 10 nM for sequencing.