RNA-seq of Physcomitrellopsis africana
Approximately 1 g of RNA was used to generate two paired-end libraries with an insert fragment size of 200300 bp of the corresponding cDNA. RNA purification, reverse transcription, library construction and sequencing were performed at WuXi NextCode (Shanghai, China). The captured coding regions of the transcriptome from total RNA were prepared using the TruSeq RNA Exome Library Preparation Kit.