Illumina short read sequencing of Staphylococcus aureus E2-4
The microbial colony was identified using a selective media and the relevant phenotypic characteristics of the colony. Total DNA was extracted from the isolated microbial colony and used as the input for library preparation. The library preparation followed the user guidelines of the Nextera XT DNA library preparation kit. The sequencing library was prepared by random fragmentation of the DNA or cDNA sample, followed by 5' and 3' adapter ligation. The library was loaded on the sequencer and sequencing occurred through the sequencing by synthesis approach.