GSM6505175: ATAC_WT_E10.5_PA2_2; Mus musculus; ATAC-seq
Embryonic tissues were micro-dissected at E10.5, E12.5 or E14.5. Dissected tissue from E12.5 embryos was kept in 1× PBS on ice, then treated with 0.5% trypsin/1× EDTA at 37 °C for 10 minutes and immediately put on ice. Dissected tissue from E14.5 embryos was kept in 1× PBS on ice, then treated with papain digestion mix (10 mg ml−1 papain, 2.5 mM cysteine, 10 mM HEPES (pH 7.4), 0.5 mM EDTA and 0.9× DMEM) for 7 min at 37 °C and immediately put on ice. The tissue was rinsed once in ice-cold 1× DMEM/10% FBS, followed by two washes in ice-cold 1× DMEM. Tissue was dissociated by pipetting. Cranial NCCs with genotype Wnt1::Cre;ROSARFP were filtered and collected by FACS. To identify open chromatin regions, we used the Assay for Transposase Accessible Chromatin (ATAC-seq) protocol, performed according to Buenrostro et al., 2015 with minor modifications as described previously in Minoux et al., 2017 (PMID: 28360266).