GSM8270120: HEK293, IFNα.20h, rep3; Homo sapiens; RNA-Seq
Total RNA was extracted from cells using Monarch Total RNA Miniprep Kit (NEB, T2010S) following the manufacturer's protocol, eluted in 100 μl nuclease-free water. Ribosomal RNAs were removed by rRNA Depletion Kit (NEB, E6310) following the manufacturer's protocol. Extracted RNAs were supplied with RiboLock RNase inhibitor (Thermo Scientific, EO0381) and subjected to SuperScript III reverse transcriptase (Invitrogen, #18080093) for cDNA library generation using random hexamer primers. RNase H was added to reverse transcription products to obtain cDNA library for RNA-seq.