GSM6591821: cortical brain tissue, B6M1; Mus musculus; RNA-Seq
16,000 nuclei per sample were loaded on the 10x Chromium chip for library preparation using the Single Cell 3' v3 chemistry according to manufacturer instructions. For cDNA amplification, 12 PCR cycles were applied. For each sample, 230 ng of purified cDNA was used for library construction. The libraries were diluted to a concentration of 10 nM and pooled for sequencing. After denaturation, the library pool was diluted to a loading concentration of 300 pM and sequenced on a Novaseq6000 in paired-end mode (2x100) (read 1:28 cycles, read 2:91 cycles, and i7 index: 8 cycles) at an average depth of 50,000 reads per nucleus. single nuclei (sn)RNA-seq