GSM6592226: blood, P137, NEC; Homo sapiens; Bisulfite-Seq
Blood genomic DNA was extracted then was quantified using the KAPA hgDNA Quantification and QC Kit. DNA was sheared with Covaris to a size of ~175bp. Libraries were prepared using the KAPA HyperPrep Kit. Libraries were bisulfite converted post-ligation using the EZ DNA Methylation-Direct Kit and amplified 10 cycles. Bisulfite-converted DNA libraries underwent targeted capture using the SeqCap Epi CPGiant Enrichment Kit and amplified 10 cycles. Samples were sequenced on a HiSeqX with 150 bp paired-end reads to a mean targeted read depth of ~56x.