RNA-Seq of Worker apron
The V4 region of the 16S rRNA gene of eluted DNA from olive flounders and their associated environmental samples were amplified via PCR using dual-index primers. Successful amplification was confirmed with gel electrophoresis on a Geldoc c150 (Azure Biosystems, California, USA),and the DNA concentration of each amplified product was equalized using the SequalPrepNormalization Plate Kit (Applied Biosystems, Massachusetts, USA). All equalized DNA samples were pooled in a LoBind microcentrifuge tube (Eppendorf, Hamburg, Germany) to create a pooled library.