GSM8385375: DHD0 (subset), DHD2, mALb (T) His+-; Saccharomyces cerevisiae; OTHER
All plasmids are extracted after growth selection using a Zymoprep Yeast Plasmid Miniprep II Kit according to the manufacturer's protocol. All proteins of interest are ordered as twist gene fragements. These fragments are ordered to contain the terminator, barcode, and overlap regions for assembly. These fragmetns are combined with the MP3-seq vector and assembled during yeast transformation through homologous recombination.