GSM8174509: IBA Subfornical Organ Replicate 3; Ictidomys tridecemlineatus; RNA-Seq
Total RNA was isolated from tissue using the Quick-RNA Microprep Kit (Zymo, R1050). RNA concentration and integrity number (RIN) were assessed by an Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA). RNA concentrations were in the range of ~8.5 - 25.8 ng/µL and RIN values were in the range of 6.8 – 9.1. Sequencing libraries were prepared using the Kapa mRNA Hyper Prep kit (KAPA Biosystems, Wilmington, MA).