scRNA-seq of homo sapiens: mucosa
Mucosal biopsies were immediately processed after endoscopy, minced with scissors and enzymatically digested on ice using Bacillus licheniformis protease, before filtering and determining cell viability and numbers. A single cell suspension of filtered epithelial cells was obtained from organoids after TrypLE digestion.Cells were encapsulated with a 10X Genomics Chromium, and after rt-PCR and cDNA amplification, libraries were prepared using 10X Genomics 3 single-cell gene expression chemistry and sequenced on Illuminas Novaseq6000 at the Yerkes Primate Research Center Genomics Core at Emory University.