GSM8387806: N_LPS_B_SI; Homo sapiens; miRNA-Seq
The Revvity Nextflex Small RNA NGS Kit v4 was employed to construct small RNA libraries from total RNA samples following the manufacturer's protocols. The procedure included adapter ligation, reverse transcription, and PCR amplification, culminating in size selection to enrich for small RNA species. Libraries were quantified, pooled (n=24), and sequenced on an Illumina platform to generate high-throughput sequencing data for downstream analysis. Library was sequenced with NextSeq 2000 P3 (50 cycle) fl The Revvity Nextflex Small RNA NGS Kit v4 was employed to construct small RNA libraries from total RNA samples following the manufacturer's protocols. The procedure included adapter ligation, reverse transcription, and PCR amplification, culminating in size selection to enrich for small RNA species. Libraries were quantified, pooled (n=24), and sequenced on an Illumina platform to generate high-throughput sequencing data for downstream analysis. Library was sequenced with NextSeq 2000 P3 (50 cycle) fl