GSM8269043: transplant-derived, BMDM, Pristane, replicate 1 [RNA-Seq]; Mus musculus; RNA-Seq
Harvested cells were pelleted and then lysed in mild lysis buffer (10mM Nacl, 10mM Tris-HSC, 0.5% NP-40, Rnase inhibitor) for 30 minutes. Cells were vortexed for 1 minute and centrifuged at 2000 g for 5 minutes at 4oC. The lysis supernatant, containing the RNA fraction was removed and immediately placed into RLT buffer from the Qiagen RNeasy micro kit and processed following manufacturer's instructions Ribo-depleted RNA isolation