GSM8179462: C3; Mus musculus; miRNA-Seq
A MagCapture™ Exosome Isolation Kit PS Ver.2 (Fujifilm Wako, 290-84103) was used to isolate exosomes. Small RNA with a size of 18–30 nt was isolated by gel electrophoresis. Small RNA Library Prep Kit (940-000196-00, MGI), and quality and quantity were assessed at all steps by capillary electrophoresis (Agilent Bioanalyzer). Libraries were sequenced on a DNBSEQ-G400 with cPAS chemistry (DNBSEQ-G400RS High-throughput Sequencing Set, FCL SE50, 1000016941, MGI).