GSM7050333: Larvae replicate 1; Drosophila melanogaster; RNA-Seq
Total RNA was isolated from embryos with Trizol (Ambion). 100 ng of total RNA was used for the construction of polyA sequencing libraries. The quality of RNA was evaluated on Fragment Analyser. mRNA libraries (polyA) for RNA-seq were prepared using NEBNext® Ultra™ II mRNA-Seq (strand specific) following manufacturer's protocols.