RNA-seq of Lingula anatina lophophore 5
Total RNA was extracted from the mantle, gonad, lophophore, adductor muscle and pedicle using TRIzol. The RNA was reconstituted in 50 l RNase-free water, and its concentration was determined with a Nanodrop spectrophotometer. RNA quality was verified using the 5400 Fragment Analyzer System (Agilent Technologies). cDNA libraries for each tissue sample were constructed with the TruSeq RNA Library Prep Kit v2. Libraries were sequenced as 150 bp paired-end reads on the Illumina NovaSeq 6000.