GSM6430454: BCBL1 shM Depletion BCBL1_Brunello_shM_cFLIP_A; Homo sapiens; OTHER
Genomic DNA was extracted from frozen pellets following a salt-precipitation protocol outlined in Manzano et al, Nat Comm 2018. For HiSeq4000 runs, a two-step PCR protocol was used for the amplification Brunello lentiGuide inserts before adding Illumina adapters and sample barcodes, as as outlined previously (Manzano et al., Nat Comm 2018). For NextSeq 500 runs, a single PCR was performed to simultaneously amplify lentiGuide inserts, add Illumina adapters, and barcode samples. OTHER: CRISPR guide amplicon sequencing