GSM7871041: Duo4_11cHi_AM; Mus musculus; RNA-Seq
Sorted eosinophils (1000 cells) were lysed in guanidine thiocyanate buffer (TCL buffer, QIAGEN) supplemented with 1% β-mercaptoethanol. RNA was isolated by solid-phase reversible immobilization bead cleanup using RNAClean XP beads (Beckman Coulter), reverse-transcribed and amplified Uniquely barcoded libraries were prepared using Nextera XT kit (Illumina) following manufacturer's instructions. Sequencing was performed on an Illumina NextSeq500 for a total yield of 400M reads