RNA-seq of mus liver03
After the total RNA of the sample was extracted, Eukaryote mRNA was enriched by magnetic beads with Oligo (dT). Adding a fragmentation buffer to break mRNA into short fragments, using mRNA as a template, synthesize the first cDNA strand using random hexamers. Then, add buffer, dNTPs, RNase H, and DNA polymerase I to synthesize the second cDNA strand. Next, perform end repair on the double stranded cDNA, add poly (A), connect sequencing connectors, use magnetic beads for purification and fragment selection, and finally perform PCR amplification to obtain the library.