GSM6510026: PCs, ADT-derived cDNA; Homo sapiens; OTHER
Ex vivo differentiated B cells/PC at day7, day 13  was removed debris by ficoll gradient and following CITEseq labeling protocol (CG000149). Sorting viable cells before loading on chromian controller. 10X CG00317 next gem single cell 3' (dual index) with feature barcode technology for surface protein CITE-seq Surface protein phenotyping: TotalSeq™-B0136 anti-human IgM Antibody, TotalSeq™-B0389 anti-human CD38 Antibody, TotalSeq™-B0055 anti-human CD138 (Syndecan-1) Antibody DAY7: TotalSeq™-B0251 anti-human Hashtag 1 Antibody for bio rep 1, TotalSeq™-B0251 anti-human Hashtag 2 Antibody for bio rep 2. DAY13: TotalSeq™-B0251 anti-human Hashtag 3 Antibody for bio rep 1 ,TotalSeq™-B0251 anti-human Hashtag 4 Antibody for bio rep 2.