GSM8174050: S11 Kras WT d7 Male AAV-Null on Kras HET genotype Kidney; Mus musculus; RNA-Seq
Whole tissue RNA was extracted using the Qiagen RNeasy kit (Qiagen, 74104), including the optional DNase step, as described previously12. Briefly, 20-30mg of snap frozen tissue were homogenized in 600ml buffer RLT/1% β-mercaptoethanol using the Precellys Evolution homogenizer (Bertin Technologies) and the RNA was eluted in 30μl RNase-free water. RNA concentration was estimated with the Nanodrop 2000 and only samples with a 260/280 ratio≥2 were used for further analysis. 20ng/ml of RNA were used to prepare libraries using the TruSeq Stranded mRNA Kit. Agilent 2200 Tapestation was used to assess library quality and Qubit (Thermo Fisher Scientific) was used to check concentration.