RNA-seq of Solanum tuberosum: leaf mock Sarpo Mira rep 1
Total RNA was extracted from the 36 leaf samples using Tri Reagent (Sigma) according to the manufacturer protocol. RNA integrity was observed by electrophoresis on a 1% agarose gel stained with GelRed (Biotium) and the RNA concentration was measured using a NanoDrop 1000 spectrophotometer. Samples were sent to the Novogene Company (Cambridge) for in-house Quality Control followed by paired-end mRNA-Seq library construction.