Viral metagenome of Eriocheir sinensis with milky disease
The hepatopancreas, branchia, and muscles of 10 crabs with milky disease were pooled and processed by  grinding and freezing-thawing repeatedly for cell lysis. We used high-speed centrifugation and Durapore PVDF 0.45m Membrane to remove eukaryotic and bacterial cell-sized particles, and then used ultra-speed centrifugation to enrich the virus-like particles. The nucleic acid was extracted using a QIAamp Viral RNA Mini Kit (QIAGEN). RNA libraries were constructed using a NEBNext Ultra II RNA Library Prep Kit for Illumina.