GSM6934563: IgD-neg, replicate 1, scRNAseq; Homo sapiens; RNA-Seq
B cells were negative selected using column purification (EasySep Pan-B cell purification kit, StemCell Technologies), treated with neuraminidase, stained with HA tetramers, fluorophore-labeled Abs, and Abs conjugated with TotalSeq C oligomer hashtags. HA-binding IgDneg CD38lo/med cells were sort-purified and barcoded. Chromium Single Cell 5' Library and Gel Bead Kit (Nest GEM 5' Kit v 1.1) as well as the Single Cell 5' Feature Barcode Library Kit (10XGenomics)