GSM8003240: vegetative cells, sad1.2 csi1Δ, biological replicate 1; Schizosaccharomyces pombe; RNA-Seq
The crashed pellet is deep-frozen again and submitted to RNA extraction protocol from the Rneasy Kit (QIAGEN, Cat. No. / ID: 74104), obtaining a sample of total RNA in 50 μL of Rnase-free water. 5 ug of this total RNA sample was enriched in mRNA through oligo dT selection and the library was prepared and sequenced as paired-end via DNB technology by the BGI platform.