="target-enrichment RawData Long-horned bees (Eucerini)"&"_"&B43
Samples were extracted using Qiagen DNeasy extraction kits. DNA was sheared to 400-600 bp using a QSoncia Q800R sonicator. Genomic libraries were prepared using Kapa Hyper Prep Kits (1/4 volume reactions) and iTru, 8bp dual-indexing adapters (Glenn et al. 2019). Samples were pooled into pools of 10 or 11 samples and enriched for ultraconserved elements (UCEs) using an bee/ant-customized RNA bait set (Branstetter et al. 2017, Grab et al. 2019). Enriched pools were pooled together (up to 102 individual samples) and shipped to a genomic facility for sequencing on an Illumina HiSeq instrument. For trimming raw reads, use i7:GATCGGAAGAGCACACGTCTGAACTCCAGTCAC*ATCTCGTATGCCGTCTTCTGCTTG and i5:AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT*GTGTAGATCTCGGTGGTCGCCGTATCATT, where * indicates the index sequence.