GSM6814260: no guide control biol rep 1; Homo sapiens; RNA-Seq
RNA was harvested in TRIzol and purified using the zymo RNA clean and concentration -5 kit, including an in column DNase treatment. Next, PolyA RNA was isolated using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490S). libraries were prepared using a NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, E7420L)