GSM8003292: control2; Rattus norvegicus; RNA-Seq
RNA was harvested using RNAiso Plus kit(TaKaRa),1.3ug of total RNA was used for the construction sequencing libraries The enriched mRNA was fragmented into short fragments using fragmentation buffer and reverse transcripted into cDNA with random primers. Second-strand cDNA were synthesized by DNA polymerase I, RNase H, dNTP and buffer. Then the cDNA fragments were purified with QiaQuick PCR extraction kit, end repaired, poly(A) added, and ligated to Illumina sequencing adapters.