GSM7016395: Kidney, LPS, sample2; Mus musculus; OTHER
In brief, sections were fixed in 100% methanol and stained with hematoxylin and eosin (H&E) reagents. H&E-stained sections were imaged using a CRi Panoramic MIDI Whole Slide Scanner with 20X magnification. Sections were then permeabilized with 0.1% pepsin in 0.01M HCl for 14 min at 37°C and processed for in-tissue reverse transcription followed by on-slide second strand synthesis. Samples were processed to generate spatial transcriptomics sequencing libraries according to the manufacturer's instructions.