RNA-seq of mock inoculated Lactuca sativa cv. Kordaat, replicate 2
Sterilised lettuce cv. Kordaat seeds were grown on ATS agar medium at 4C in the dark for 4 days. Plates were then incubated at 15C in 16 h light/dark for 8 days, before the temperature was increased to 25C for six days. Mock inoculations were pipetted directly onto the lettuce roots and spread by tilting, before being dried briefly under sterile air flow. Lettuce roots were harvested 96 hours post-inoculation prior to RNA extraction and sequencing.