GSM7012035: zebrafish embryo, 1hpf, rep2; Danio rerio; RNA-Seq
Total RNA was isolated using tri reagent (Sigma T9424) and quantified using nanodrop polyA+RNA: Indexed RNA-Seq libraries were prepared from 500 ng total RNA using the KAPA Stranded mRNA-Seq Kit (Kapa Biosystems, Inc.) according to the manufacturer's protocol. Libraries were size-selected with SPRI beads and quantified by QuBIT (Life Technologies). Average size of 300 bp was determined by TapeStation (Agilent Technologies, USA). Libraries are then sequenced using an Illumina Nextseq500 sequencer. total-RNA: indexed RNA-Seq libraries for each sample were generated from total RNA using the Illumina RiboZero(Human/Mouse/Rat) kit following the manufacturer's instructions. Libraries were size-selected with SPRI beads and quantified by QuBIT (Life Technologies). Libraries are then sequenced using an Illumina Novaseq sequencer.