GSM7870876: hESC_P_body_rep1; Homo sapiens; RNA-Seq
RNA was harvested using TRIzol (Invitrogen), for P-bodies samples, ~10-30ng RNA were used for the construction of sequencing libraries, for cytoplasm or whole cells samples, 2μg RNA  were used for the construction of sequencing libraries. RNA libraries for RNAseq were prepared using Universal V8 RNA-seq Library Prep Kit for Illumina (VAHTS) following manufacturer's protocols