GSM6894115: SCD post AMI, D36; Homo sapiens; RNA-Seq
Total RNA from peripheral blood mononuclear cells (PBMCs) was isolated using TRIzol reagent and the libraries were constructed using TruSeq Stranded mRNA LTSample Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer's instructions and sequenced on an Illumina HiSeq X Ten platform and 150 bp paired-end reads were generated. The transcriptome sequencing and analysis were conducted by OE biotech Co., Ltd. (Shanghai, China). Raw data (raw reads) were processed using Trimmomatic. The reads containing ploy-N and the low quality reads were removed to obtain the clean reads. Then the clean reads were mapped to reference genome using hisat2.