Spatial transcriptomics of kidney needle biopsy: pre transplant
Slide preparation (CG000240_Demonstrated_Protocol_VisiumSpatialProtocols_TissuePreparationGuide, 10X Genomics), imaging (CG000241_VisiumImagingGuidelinesTN_RevD, 10X Genomics), mRNA extraction, library preparation, and sequencing (CG000239_Visium Spatial Gene Expression User Guide_Rev F 10X Genomics) were conducted according to Visium Spatial Gene Expression protocols .