GSM6896007: Wild-type plants under drought conditions, IP_DNA, K4 rep 2; Populus trichocarpa; ChIP-Seq
Xylem tissue was cross-linked in 1% formaldehyde and used to isolate nuclei and chromatin. The chromatin was sheared into 200- to 1000-bp fragments, subjected to immunoprecipitation using Anti-H3K4me3 antibody (Abcam, ab8580), and collected with protein G magnetic beads (Invitrogen). Precipitated chromatin was de-cross-linked to release the ChIP-DNA. Libraries were prepared using a library preparation kit (New England Biolabs) according to the manufacturer's instructions. 20 ng of DNA was used as starting material for input and ip samples. DNA fragments around 200 bp in length were selected and amplified 9 cycles on the thermocycler.  Libraries were validated using the Agilent High Sensitivity DNA Kit.