GSM8003548: MRC5 cells, untreated control, 24H - biol rep 3 [M_24_03]; Homo sapiens; RNA-Seq
Total RNA was isolated using a Ribospin Kit (#304-150, GeneAll Biotechnology). 1.2 ug of total RNA was used for the construction of sequencing libraries. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library.