GSM8174072: Stim_AAVS1_2; Homo sapiens; RNA-Seq
The mRNA of AAVS1-KO and CALHM2-KO primary NK cells with or with cancer stimulations were extracted with RNeasy plus kit (Qiagen) according to manufacturer's instruction. The mRNA library preparations were performed using a NEBNext® Ultra™ RNA Library Prep Kit, and samples were multiplexed using barcoded primers provided by NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2).