GSM7385431: CHiC_H_E115-WT; Mus musculus; OTHER
Tissues were homogenized using a Dounce tissue grinder and fixed with 2% formaldehyde (37%) for 10 min. The reaction was quenched using 1.25M Glycine and pellets were snap-frozen for storage at -80°C. For nuclei isolation pellets were resuspended in custom-made lysis buffer (10mM Tris pH7.5, 10mM NaCl, 5mM MgCl2, 0.1mM EGTA, Protease Inhibitor). After on wash with PBS samples were digested with DpnII, re-ligated, de-crosslinked and purified. The 3C-library was sheared and after adaptor ligation and amplification of sheared DNA fragments, libraries were hybridized to custom-designed SureSelect beads (SureSelectXT Custom 0.5-2.9Mb library) and indexed following Agilent's instructions. Multiplexed libraries were sequenced using a Illumina HiSeq 4000 (50bp paired-end). 3C-library, hybridization to custom-designed SureSelect beads (Agilent)