16SrRNA of mucosa: adult human colorectum
The hypervariable region V3-V4 of the bacterial 16S rRNA gene were amplified with primer pairs 331F (5'-TCCTACGGGAGGCAGCAGT-3') and 797R (5'-GGACTACCAGGGTATCTAATCCTGTT-3') by an ABI GeneAmp 9700 PCR thermocycler (ABI, CA, USA).