GSM8048169: NSD2∆IE; Mus musculus; OTHER
Colon tissue was separated from mice, cleaned, and quickly stored in a special preservation solution. CUT&Tag library construction using the kit Hyperactive TM In-Situ ChIP Library Prep Kit for Illumina (Cat. No. TD901-TD902, Vazyme Biotech Co., Ltd) Concanavalin A-coated magnetic beads (ConA beads) were used to bind the cells, and the nonionic detergent digitonin (Digitonin) was used to permeabilize the cell membrane; the cells bound to the magnetic beads were sequentially Incubate with (enter target protein name) primary antibody (enter primary antibody product number and company), secondary antibody and Protein A/Protein G fused with Tn5 transposon (Hyperactive pA/pG-Tn5 Transposon), so that with Protein A/ The Protein G-fused Tn5 transposon precisely targets and cuts the DNA sequence near the target protein; when Tn5 cuts, it will add P5 and P7 adapter sequences to both ends of the cut fragment, and use P5 and P7 primers for PCR amplification. After the purified sequencing library passed the quality inspection of Agilent 2100 Bioanalyzer, the Illumina NovaSeq6000 sequencing platform was used for high-throughput sequencing to generate 150 bp paired-end data for subsequent analysis.