CRISPR/Cas9 mutation readout on the pathway reporters in hTERT RPE-1 p53KO or p53/BRCA1dKO on sample: hTERT_RPE1_Cas9_p53KO_DSBTRIP_manual_BOD1L1_KO_pool_LBR2_72h_Run1
CRISPR/Cas9 mutation readout on the pathway reporters in hTERT RPE-1 p53KO or p53/BRCA1dKO after individual DNA repair protein knock-outs. Five days after knock-out induction, a second gRNA is transfected to induce DSB in pathway reporters in parallel. Three days later, cells are harvested for mutation readout PCR.