long reads nanopore sequencing
SQK-SKQ110 kit was used following the Oxford Nanopore  Genomic DNA by Ligation (SQK-LSK110)  protocol. One aliquot was loaded onto one R9.4.1 PromethION flow cell. The other aliquots of the libraries were used for reloading the flow cell after two DNase I treatments during the run. Reads were base-called using Guppy version 3.2.10+aabd4ec for the five first runs and Guppy version 4.0.11+f1071ce for the last one.