Mock-Infected WT AB Zebrafish Larvae 2 dpi Replicate 3
The goal of this experiment was to characterise the response to Cyprinid Herpesvirus-3 (CyHV-3) infection in WT AB zebrafish larvae in terms of the interferon stimulated gene (ISG) upregulation and the potential involvement of programmed cell death. Zebrafish larvae were either infected with CyHV-3 or mock infected with PBS at three days post fertilization (dpf). Larvae were sampled at 1, 2 and 4 days post infection (dpi). Samples were taken in triplicate at each time point (each replicate consisted of five pooled larvae). For each sample, immediately after euthanasia, five whole larvae were lysed together in 700 μL QIAZol (Qiagen) and lysate was stored at -80C. After phase separation, RNA was extracted from the aqueous layer as per RNeasy kit with on-column DNase digestion. Samples were analysed by Agilent 2100 Bioanalyzer to confirm that all samples exhibited RIN values >9.5. RNA sequencing libraries were prepared using TruSeq Stranded mRNA kit (Illumina), as per manufacturers instructions. Sequencing was conducted using the Illumina NextSeq 500 System. Data consists of original raw output from RNAseq with no adapter or quality trimming (.fastq fromat). Please refer to publication for further details.