G6PD deficiency
Genomic deoxyribonucleic acid (DNA) was extracted from the peripheral blood of the patient and his parents using the QIAamp DNA Blood Mini kit (Qiagen, Hilden, Germany). DNA quality was assessed using a NanoDrop ultraviolet spectrophotometer (Thermo Fisher Scientific, USA). Whole-exome sequencing (WES) and analysis protocols were adapted for genetic analysis. Enriched DNA samples were indexed and sequenced on the HiSeq 2000 platform (Illumina, San Diego, CA) in accordance with the manufacturers instructions. Nucleotide changes observed in more than 5% of aligned reads were identified and reviewed using NextGENe software (SoftGenetics, State College, PA). Sanger sequencing was performed to confirm the mutation of G6PD. The impact of the missense mutation on protein function and structure was assessed by PyMOL software (Schrdinger, USA)