Metabarcoding of bulk ichthyoplankton
DNA extracted from homogenized bulk ichthyoplankton or fin clips from adult specimens using Qiagen DNeasy Blood & Tissue Kit, 1st PCR amplification using HuRhon Rhodopsin metabarcoding primers with heterogeneity spacers and TruSeq adaptor sequences, 2nd PCR using combinatorial TruSeq i5/i7 indices, purified using Ampure XP magnetic beads and quantification via QuBit broad range kit, Illumina MiSeq using the V2 (150bp x 2)