GSM6503430: PC9-Ge-2; Homo sapiens; RNA-Seq
H1975 or PC9 cells were treated with Osi (500 nM) or Ge (100 nM) for 3 days, after which apoptotic cells were washed away with ice-cold PBS, and the total RNA of the remaining cells together with the cells before treatment was extracted using TRIzol reagent. The Total RNA was incubated for 30 min at 37 °C with 10 units RNase R (Geneseed) .Then，RNA-seq library was prepared with approximately 2μg of total RNA usingRNA-seq (H/M/R) Library Prep Kit for Illumina® (Geneseed).