GSM8269171: Hyperoxia_Male_3; Mus musculus; RNA-Seq
Total RNA from sorted alveolar macrophage cells were isolated using the RNeasy Plus Micro Kit (74034, QIAGEN). Sample concentration and quality checks were done using the Agilent Bioanalyzer. The libraries were prepped using the SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing 634894, TAKARABIO. The first 8 nt were UMIs followed by the Pico v3 SMART UMI Adapter, both of which were trimmed. Novg libraries were purified further to remove fragments with less than 250bp and resequenced.