GSM8388796: M155; Mus musculus; RNA-Seq
Whole synovium, including Hoffa's fat pad, was microdissected from male and female Sham, 7d and 28d ACLR, and contralateral knee joints, then immediately placed in TRIzol on ice. Samples were homogenized in TRIzol using an automated tissue homogenizer. RNA was isolated using Qiagen RNeasy Micro kits, and all samples were confirmed to have RNA integrity number (RIN) values > 6.5. Libraries were constructed according to the standard BGI protocol, where the insert is flanked by adaptors: 5' read 1 or 3' read 2, index sequence, and BGI barcode primer