Illumina WGS of GM24631 genome after base editing and USER treatment
Purified DNA from GM24631 was incubated with purified ZF-CBE and then purified using isopropanol/ethanol precipitation. DNA was then incubated with USER then purified using isopropanol/ethanol precipitation. Sequencing libraries were prepared using Illumina's TruSeq PCR-free DNA prep kit and a ME220 sonicator with indicated settings for 550 bp inserts. TruSeq DNA UD indices were used to add sample-specific barcodes. Reads were demultiplexed and quality filtered.