GSM7804586: APOBEC1_MAVS-FL_RepA; Homo sapiens; RNA-Seq
RNA was isolated using TRIzol. DNA was eliminated using Turbo DNase I, and RNA was cleaned by phenol-chloroform extraction and ethanol precipitation. Library construction was performed through BGI Genomics. Briefly, rRNA was eliminated by digestion with RNase H and RNA was fragmented. First strand cDNA synthesis was performed using random hexamer-primed reverse transcription, followed by a second-strand cDNA synthesis with dUTP instead of dTTP. After end repair, A-addition, and adaptor ligation, U-labeled second strand cDNA was digested with UDG and samples were amplified by PCR. After QC, library was cyclized using a splint oligonucleotide and DNA ligase. DNA nanoball libraries were then synthesized and sequenced on the DNBseq platform.