GSM8387779: 41D,F; Gasterosteus aculeatus; RNA-Seq
One hour after the trial, males were sacrificed (n = 8 after a courtship opportunity, n = 8 after predation risk, n = 8 after a tradeoff, n = 7 after a control) and their telencephalon and diencephalon were rapidly dissected and deep frozen at -80°C until extraction. RNA was extracted using Invitrogen PureLink RNA extraction kits (Invitrogen Corporation, Carlsbad, CA) and quality was checked using Agilent Bioanalyzer chips. Library prep and sequencing was performed by the Functional Genomics Unit of the W.M. Keck Center (University of Illinois Urbana Champaign). Libraries were prepared using the 'Kapa Hyper Stranded mRNA library kit' (Roche) and pooled, quantitated by qPCR and sequenced on one S4 lane on a NovaSeq6000 to a depth of >30M reads.