GSM7385433: ATAC_H_E115-WT_rep2; Mus musculus; ATAC-seq
Tissues were dissociated in Lysis buffer using a Dounce tissue grinder and approx. 50000 cells were pelleted and resuspendend in 50uL transposition reaction mix. Samples were incubated in transposition reaction mix (25 μL Nextera 2x TD buffer and 2.5 μL TDE1; Illumina) for 30 minutes at 37C with shaking. Following amplification, ATAC-libraries were purified, quantified and quality assessed using a Agilent Bioanalyzer. Libraries were pooled and sequences on an Illumina HiSeq 4000 (single end 50bp).