GSM8305946: Culex adults, insulin, WNV, 24 h, biol rep 4; Culex quinquefasciatus; RNA-Seq
Animals were homogenized in Trizol reagent. RNA was harvested using Trizol reagent and Direct-Zol RNA extraction kit (Zymo Research). 2.5 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using TruSeq Stranded mRNA Library Prep Kit (Illumina) following manufacturer's protocols.