GSM6429707: RPE-1_H3K27me3_rep2; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a E220 focused-ultrasonicator (Covaris). H3K27me3 (Millipore 07-449), H3K36me3 (Abcam 9050), H3K4me1 (Abcam 8895), H3K4me3 (Abcam 8580) ChIP-seq samples were used from (https://doi.org/10.1101/2021.02.02.429315). 65bp single-end reads were trimmed to 54bp reads and aligned to hg38 using BWA (v0.7.17-r1188). Mapped reads were filtered for mapping quality using samtools < MQ20.  samtools version 0.1.19.Only r1_were used. H3K27ac, H3K9me3 and H2A.Z ChIP-seq samples were processed for library preparation (Part# 0801-03003, KAPA Biosystems kit) and sequenced with Illumina NovaSeq6000 54bp paired-end reads. Mapped reads were filtered based on mapping quality of 20 using samtools version 0.1.19. Only r1_were used.