GSM6621003: ChIPSeq-HAP1-siMZ-IpH3K4me3-lib5-51821_S29_L005; Homo sapiens; ChIP-Seq
Chromatin immunoprecipitation (ChIP) was performed with a ChIP-IT Express kit following manufacturer's instructions. Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a Bioruptor plus (Diagenode). ChIP-Seq libraries were prepared using the “NEBNext® Ultra™ II DNA Library Prep Kit”.  Libraries were amplified using 15 cycles on the thermocycler. Post amplification libraries were size selected at 250-450bp in length using Agencourt AMPure XP beads from Beckman Coulter. Libraries were validated using the Agilent High Sensitivity DNA Kit.