GSM8178212: Col0 roots biol rep 2; Arabidopsis thaliana; RNA-Seq
Three biological replicates of around 60 plants each were prepared, and the total RNA of 8 dps (days post sowing) roots were extracted using the Quick-RNA Miniprep Plus Kit (Zymo Research, Irvine, USA), including a DNaseI treatment step to remove any contaminating DNA. The RNA integrity and quantity was checked using a 2100 Bioanalyzer. The libraries were constructed as indicated by the Illumina TruSeq Stranded mRNA Reference Guide for paired-end sequencing (2017).