GSM6503226: CCR10+ Tregs, biol rep 1; Homo sapiens; RNA-Seq
Three healthy control individuals donated blood from which PBMCs were isolated and cryopreserved. After thawing, cells underwent staining with antibodies to identify regulatory T cells (live, CD45+CD3+CD4+CD25+CD127-) along with CCR10. Cell were subsequently flow sorted for CCR10+ and CCR10- Tregs. From each cell pellet, RNA was isolated using QiaSymphony RNA extraction kit (Qiagen) according to manufacturer's instructions. Library prep was performed with the Ovation low input RNA sequencing kit (Tecan) according to manufacturer's instructions.