Spatial Transcriptomics (10xVisium) of F. hepatica: section D
Poly-adenylated mRNA deriving from permeabilized tissue sections was captured by spatially barcoded oligonucleotides (oligo-dT) on a glass slide. Reverse transcription generated full legth, barcoded cDNA, which was then applified by PCR to generate sufficient mass for library construction. The library comprised standard Illumina paired-end constructs, flanked with P5 & P7. Read 1 (28bp) is used to sequence the 16bp Spatial Barcode and 12bp UMI, Read 2 (90bp) sequences the cDNA insert. The sample was sequenced on two lanes in parallel