GSM7385413: MEP Replicate 1; Homo sapiens; RNA-Seq
Individual cell populations were isolated via FACS from CD34-selected G-CSF mobilized peripheral blood. Total RNA was then extracted using the RNAqueous-Micro kit (Invitrogen) per manufacturer's protocol. Libraries were prepared using the Illumina TruSeq Stranded mRNA kit. Sequencing was conducted using standard Illumina protocols.