GSM6592508: Z141_RD_3; Linum usitatissimum; Bisulfite-Seq
DNA extracted using CTAB method, the specific experimental steps are as described by Clarke(Clarke, 2009). The Bisulfite-treated linseed DNA samples were used to prepare methylC-seq libraries. A total of 16 DNA samples (one control and three different drought treatments × two biological × two linseed varieties ) were sent to BioMarker Biotechnology Co., Ltd. (Beijing) for sequencing library construction and MethylC-sequencing. Sequencing libraries were prepared using a NEXTflex™ Bisulfite Sequencing Kit (PerkinElmer, USA) following the manufacturer's recommendations, followed by MethylC-sequencing using the Illumina NovaSeq 6000 platform (San Diego, USA)