GSM8389452: N354, scRNAseq; Canis lupus familiaris; RNA-Seq
Five mm diameter neuretinal punches were collected and pretreated with 0.25% trypsin for 10 min at 37C. The tissue was then dissociated into single cells using the Miltenyi Biotec Neural Tissue Dissociation kit - Postnatal neurons. Cells were washed in PBS and resuspended in PBS supplemented 0.5% BSA. Libraries were prepared using 10X Single Cell 3' v3.1 following manufacterer's protocol.