PacBio sequencing of Nicotiana attenuata
Nicotiana attenuata plants used sequencing 30th generation inbred line referred to as the UT accession, which originated from a 1996 collection from a native population in Washington County, Utah, USA. Plants were grown with a day/night cycle of 16 h (2628 C)/8 h (2224 C) in glasshouse of Max Planck Institute for Chemical Ecology, Jena, Germany. High molecular weight gDNA for PacBio sequencing was isolated by Amplicon Express (https://ampliconexpress.com). Data were generated using Pacific Biosciences SMRT sequencing (PacBio) on RSII PacBio sequencers using RSII P6/C4 chemistry at DRESDEN-concept Genome Center. Three libraries were prepared and 192 SMRT cells were run. About 60x coverage was achieved during sequencing.