GSM6774797: Br_LFDxLFD_--_2; Mus musculus; RNA-Seq
RNA was extracted from TRIzol using standard protocols. The Illumina TruSeq kit was used to create polyA+ libraries from total RNA. We generated strand-specific libraries for tissues collected form the F1 crosses (TruSeq stranded Illumina) and non-strand-specific TruSeq libraries for the adult organs. All the libraries were run on a Bioanalyzer to assess purity, fragment size, and concentration and sequenced on a HiSeq 2500 at Harvard University's Bauer Sequencing Core (75 bp paired end).