16S rRNA gene amplicon sequencing of lake shore soil: Mesocosm control, T=95
DNA was extracted from soil samples (1 g) using the Qiagen PowerSoil kit (Qiagen) and following the manufacturer's protocol. Approximately 5ng of DNA were used as template to amplify the V4-V5 region of bacterial 16S rRNA genes with the primers 515F-Y and 926R.