GSM6506175: WT-tumor_V1G_1a; Mus musculus; RNA-Seq
On day 28 post inoculation, single cell suspensions were prepared from the tumors after mechanical disruption and enzymatic digestion with 1 mg/mL Type IV Collagenase Experimental procedures for scRNA-seq followed established techniques using the Chromium Single Cell 3' Library V3 Kit (10x Genomics). Total tumor cells or FACS-sorted CD45+ tumor infiltrating leukocytes were loaded onto a 10X Genomics Chip A and emulsified with 3' Single Cell GEM beads using a Chromium™ Controller. Libraries were constructed from the barcoded cDNAs (Ohio State University Comprehensive Cancer Center) and sequenced for approximately 300 million reads/sample on a NovaSeq S4 flow cell (Illumina) at the Core facility (The Ohio State University Comprehensive Cancer Center).