RNA-seq of Laminaria ochroleuca
Total RNA extraction was conducted following the method described by Heinrich et al., (2012). RNA quality (OD260/280 ration values) and concentration (ng/l) were obtained using a DeNovix DS-11 Series Spectrophotometer/Fluorometer (22.96 ng/l  for Chondrus crispus; 235.47 ng/l  for Laminaria hyperborean; 94.697 ng/l  for Laminaria ochroleuca). One sample of total RNA of each target species was used for cDNA libraries preparation, using a TruSeq Stranded Total RNA Library protocol with RiboZero Plant, and short reads paired-end sequences were generated in an Illumina HiSeq4000 platform, at Macrogen, Inc.