GSM6899858: HuhST2KO_IFNy_4h_rep2; Homo sapiens; RNA-Seq
Total RNA was isolated using TRI-REAGENT (MRC) followed by a column-based Total RNA Zol-Out™ D kit (A&A Biotechnology) following the manufacturer's protocol RNA-seq libraries were prepared in two biological replicates from 1ug of total RNA using NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (NEB) together with NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) and NEBNext® Multiplex Oligos for Illumina® (NEB) according to manufacturer's protocol