GSM7872454: WT-H-P; Mus musculus; RNA-Seq
six- to eight-week-old mice were randomly divided into control and model groups. All mice were anesthetized with 150 μl 5% (m/v) chloral hydrate via intraperitoneal injection and sensitized intranasally from one nostril using 250 µg/kg HDM in 20 µl PBS on day 0. On days 7–11, mice were challenged daily with 250 µg/kg HDM in 20 µl PBS intranasally. On day 13, mice were intranasally administered PR8 (1×106 PFU/kg) Total RNA was isolated using TRIzol reagent (Invitrogen, Carlsbad, California). RNA-seq detection and data processing were performed by the Shanghai Applied Protein Technology Corporation (Shanghai, China). For data quality control, genes were pre-filtered by counts >20 and reads per kilobase million (RPKM) >1 in at least one sample. Differentially expressed genes were identified using DESeq2 with a fold change >2 and an adjusted P value (FDR) <0.01, and the P value was calculated using the Z test (Pearson's chi-square test) in the Agilent GeneSpring GX suite.