GSM8388890: UPN662_T2_mERRBS; Homo sapiens; Bisulfite-Seq
DNA were extracted from bone marrow cells of patients using All Prep DNA/RNA Mini Kit (Qiagen). 100ng of high quality genomic DNA were digested with 200U of methylation-insensitive endonuclease enzyme MspI (New England Biolabs, NEB),end repaired, A-tailed and ligated to RNA TruSeq Illumina indexed adapters (Illumina, RS-122-2001). Library fragments were gel isolated from a 1.8% agarose gel (Low range Ultra aga-rose, Biorad) using Qiaquick Gel Extraction kit and bisulfite converted using EZ DNA Methylation kit (Zymo Research). Products were amplified by PCR and final libraries were purified using AMPure XP beads (Agencort). mERRBS libraries were prepared for sequencing using manufacturer's recommended protocol for 50bp stranded single-end read runs (Illumina San Diego, CA)