GSM7869824: X19421; Homo sapiens; OTHER
Human endometrial biopsies fixed in formalin and embedded in parafin were sectioned and H&E stained directly on 10X Genomics Visium Gene Expression slides and imaged using an EVOS (M7000) at 10x magnification. Tissues were permeabilised and RNA was subjected to spatial transcriptomics as per manufactuers protocols. Human whole transcriptome probe panels, consisting of a pair of specific probes for each targeted gene, were added to the tissue. These probe pairs hybridize to their gene target and are then ligated to one another. The ligation products are released from the tissue upon RNase treatment and permeabilization. The ligated probe pairs bind with spatially barcoded oligonucleotides present on the Capture Area. All the probes captured by primers on a specific spot share a common Spatial Barcode. Libraries are generated from the probes and sequenced and the Spatial Barcodes are used to associate the reads back to the tissue section images for spatial mapping of gene expression.