16S Metabarcoding of soil from earthworm mesocosm experiment :  NCM burrow
Libraries were constructed using the 341F/785R primers containing forward (5-ACACTGACGACATGGTTCTACA-3) and reverse (5-TACGGTAGCAGACTTGGTCT-3) overhang adapters. Two PCRs were run separately, the amplicon quality was assessed on a 1% agarose gel and the replicate amplicons were pooled and sent to the Gnome Qubec Innovation Center for normalization, barcoding, multiplexing and Illumina paired-end (2  300 bp) MiSeq sequencing.