GSM8268887: IVT_DRS_ac4C; Oryza sativa; RNA-Seq
The IVT products were treated with RNase-free DNase I (NEB, M0303S, USA) to remove residual DNA, and then purified using the RNeasy Mini Kit (QIAGEN, 74104, USA). The tailing reaction was catalyzed by E. coli Poly(A) Polymerase (NEB, M0276S, USA). The products were purified again using the RNeasy Mini Kit, followed by overnight ethanol precipitation to obtain poly(A)-tailed RNA for nanopore direct RNA sequencing. Sequencing libraries were prepared according to the ONT's SQK-RNA002 protocol. Nanopore Direct RNA-seq