GSM7870841: #4-17 TCR; Homo sapiens; RNA-Seq
On day 10 of the culture, cells were washed and stained with anti-human CD3 and TotalSeq-C antibodies (see ADT/HTO_ information.csv). Proliferated T cells (CD3+CTVlow) were sorted and used for single-cell TCR and RNA sequencing analyses. Single cell library was prepared using the reagents from 10x Genomics following the manufacturer's instructions. After reverse transcription, cDNA was amplified for 14 cycles, and up to 50 ng of cDNA was used for construction of gene expression and TCR libraries. Libraries were sequenced in paired-end mode.