GSM7660323: hiPSC-NSC HD 2.3; Homo sapiens; RNA-Seq
hiPSC-NSC were enzymatically dissociated with Accutase while hfNSC were mechanically dissociated by pipetting. Cells were then checked for viability (>90%), counted, and resuspended at a concentration of 1,000 cells/μl Single cells were processed using the Chromium Single Cell 3' Reagent Kit (v3.1 Chemistry) and the 10x Chromium Controller platform (10X Genomics). The target cell recovery was 2000 cells. Samples were processed following the manufacturer's protocol. The resulting libraries were assessed for size distribution and concentration using the HS DNA assay (Agilent) on the TapeStation platform (Agilent)