GSM7659988: AB5075_sac_1; Acinetobacter baumannii AB5075; RNA-Seq
Cultures were harvested by centrifugation and washed in RNAlater to preserve RNA integrity. RNA was isolated using athe Qiagen RNAeasy Kit with on-column DNAase digestion (Qiagen). RNA quality was assessed using a Bioanalyzer. Samples were additionally treated with RNAse-free DNAse (Invitrogen). cDNA library was prepared with Illumina's Stranded Total RNA Prep Ligation with Ribo-Zero Plus kit and 10bp IDT for Illumina indices.