DNA-Seq of human placental microbiome: pre-term delivery
Placental dissection was done immediately after delivery. The fetal membranes were trimmed and a 3x3 cm sample was snap frozen. The samples were stored at -20C until sample processing. DNA was isolated using the MagMAX Total Nucleic Acid Isolation Kit and quantified using a Qubit 3.0 fluorometer. Quantified and pooled libraries were clustered on the Illumina HiSeq 4000 for 150bp PE sequencing.