GSM6622152: AEG-1-C75S-replicate 1; Mus musculus; RNA-Seq
Total RNA was extracted using the QIAGEN miRNAeasy Mini Kit (QIAGEN, Valencia, CA). cDNA preparation was done using ABI cDNA synthesis kit (Applied Biosystems, Foster City, CA). Q-RT-PCR was performed using an ABI ViiA7 fast real-time PCR system and Taqman gene expression assays according to the manufacturer's protocol (Applied Biosystems, Foster City, CA). RNA-Seq library was prepared using Illumina TruSeq RNA sample preparation kit and subjected to sequencing on Illumina HiSeq3000 platform.