GSM6817355: WT_22DPA_Input; Fragaria vesca; OTHER
Total DNA was extracted from fruits of 22 DPA WT fruits using the Hi-DNAsecure Plant Kit (TIANGEN, China). Genomic DNA library was prepared using NEBNext®DNA Library Prep Master Mix Set Kit for Illumina®. FvRIF-HaloTag recombinant protein was purified with HaloTag Beads, and the FvRIF-HaloTag beads mixtures or HaloTag beads (input negative control) were directly used to incubate with the genomic DNA library. After washing five times with PBS buffer, the DNA fragments were eluted from the FvRIF-HaloTag beads by the elution buffer. DAP-seq library was constructed using KAPA Library Quantification Kit (KAPA, USA) and then subjected to high-throughput sequencing analysis on Illumina Nova S4 (Illumina, USA).