GSM6817248: Kidney, Pkd1 KO, replicate 9; Mus musculus; RNA-Seq
The total RNA was extracted with Trizol reagent following standard protocols, after which samples were DNase treated to minimize genomic DNA contamination. The integrity of the RNA sample was assessed using the Fragment Analyzer (Agilent Technologies) to ensure that the RNA integrity number (RIN) was sufficient for library preparation RNA libraries for RNA-seq were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina following manufacturer's protocols.