RNA-Seq of Capan-1
Total RNA was isolated using the TRIzol method, and RNA integrity was assessed using Agilent's TapeStation RNA screentape (Agilent, #5067-5576). Only samples with a RNA Integrity Number (RIN) exceeding 7.0 were deemed suitable for RNA library construction. RNA-seq libraries were meticulously crafted utilizing the TruSeq Stranded mRNA Sample Prep Kit (Illumina, Inc., San Diego, CA, USA, #RS-122-2101). These libraries underwent quantification via KAPA Library Quantification kits and quality assessment using a TapeStation D1000 ScreenTape (Agilent Technologies, #5067-5582) prior to sequencing on an Illumina NovaSeq platform.