GSM7660035: Healthy_donor_1; Homo sapiens; miRNA-Seq
Exosomes were isolated using the EXO-Prep exosome precipitation kit (HansaBioMed Life Sciences, Tallinn, Estonia) according to the manufacturer's instructions. The pelleted exosomes were resuspended directly into Trizol reagent (15596026, Invitrogen) with one 30-second pulse with LABSONIC M sonicator (Sartorius) to homogenize the solutions. Subsequently, the RNA was isolated according to the manufacturer's instructions with one minor modification of addition of Linear Acrylamide (AM9520, Invitrogen) in the aqueous phase to a final concentration of 20μg/ml Small RNA libraries were prepared with the Ion Total-RNA seq kit v2 (4479789, Ion Torrent) according to the manufacturer for small RNA libraries without enrichment (Rev. B.0)