Whole Genome Sequencing of Penicillium citrinum B9
In the case of Illumina data, the sequencing library is prepared by random fragmentation of theDNA or cDNA sample, followed by 5 and 3 adapter ligation. This library is loaded into a flow cellwhere fragments are captured on a lawn of surface-bound oligos complementary to the libraryadapters. As all 4 reversible, terminator-bound dNTPs are persent during each sequencing cycle,natural competition minimizes incorporation bias and greatly reduces raw error rates compared toother technologies. Then, sequencing data is converted into raw data for the analysis.