GSM6622108: mRNA-Seq Old replicate 2; Mus musculus; RNA-Seq
The isolated pituitary from young (2-3M) or old (18-24M) mouse was directly lysate in lysis buffer and RNA was extracted using RNAprep pure Cell/Bacteria Kit following the manufacturer's instructions. Concentration of RNA was measured and the sample passed quality checks was used for RNA sequencing library preparation. Libraries for mRNA abundance examination was prepared by using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB).