GSM8389175: CD4+ T cell, HDAC1-cKO, day 10, rep2; Mus musculus; RNA-Seq
Total RNA extraction was done with ReliaPrep RNA cell miniprep system kit (Promega) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end pairing, A-tailing, adapter ligation, size selection, amplification, and purification.