GSM7050015: M34 Amplicon-NGS on treated cells (post transplantation); Mus musculus; OTHER
Genomic DNA was isolated from cells using theNucleoSpin Tissue Mini kit for DNA from cells and tissue (Macherey-Nagel) according to the manufacturer's instructions. DNA library preparations, sequencing reactions, and initial bioinformatics analysis were conducted at AZENTA, Inc. (Leipzig, Germany). DNA Library Preparation, clustering, and sequencing reagents were used throughout the process using NEBNext Ultra DNA Library Prep kit following the manufacturer's recommendations (Illumina, San Diego, CA, USA). End repaired adapters were ligated after adenylation of the 3'ends followed by enrichment by limited cycle PCR. DNA libraries were quantified using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA) and multiplexed in equal molar mass. The pooled DNA libraries were loaded on the Illumina instrument according to manufacturer's instructions. The samples were sequenced using a 2x250 paired-end (PE) configuration. Image analysis and base calling were conducted by the Illumina Control Software on the Illumina instrument.