GSM7802846: CHP134 cells, DMSO treated, biological replicate 1; Homo sapiens; RNA-Seq
RNA was extracted using TRIzol.  1ug of RNA was used to construct sequencing libraries oligo dT based mRNA enrichment was used prior to RNA fragmentation and reverse transcription with random N6 primer. Second strand synthesis was performed. End repair and addition of A and adaptor ligation proceeded PCR amplification.  Single strands were heat separated and circularised for DNA nanoball synthesis and sequencing on DNBseq platform