TCR beta sequencing of perivascular adipose tissue: abdominal aortic diseases
The Oncomine TCR Beta-LR Assay was used with multiplexed primers targeting the F1 region of the variable V segment gene and the constant segment gene (64 and 2 primers respectively) of the TCR-VDJ rearrangement in cDNA. This system produces an amplicon of ~330 base pairs and allows sequencing of all three complementarity determining regions (CDR1, CDR2 and CDR3).