GSM8386445: hESC-CM linc01629-KD rep2; Homo sapiens; RNA-Seq
The H1-dCas9-KRAB hESC lines were infected with Non-Targeting Control (NTC) and LINC01629 gRNAs, then differentiated into cardiomyocytes and matured for 60 days before harvesting for bulk RNAseq and differential expression analysis. RNA was isolated using the Direct-Zol RNA Miniprep kit. Total RNA library preparations were prepared using TruSeq Stranded Total RNA Library Prep HMR kit (Illumina, 20020596) and respective cDNA libraries were prepared by Macrogen Asia Pacific Pte. Ltd. RNA libraries were sequenced on HiSeq 4000 Illumina sequencing platform to achieve a sequencing depth of at least 50 million paired-end reads per biological sample.