RNA-Seq of primary cultured microglia
Total RNAs from primary cultured microglia were purified using RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions. Libraries were prepared with MGIEasy RNA Directional Library Prep Kit V2.0 (MGI Tech Co.) and sequenced with 151-nt paired-end reads using DNBSEQ-G400 (MGI Tech Co.).