GSM8048153: Pelabresib+Enza 1; Homo sapiens; RNA-Seq
To isolate RNA from xenograft tumor explants, the tumor samples were ground in 1 ml Trizol (Invitogen) using a PowerGen homogenizer (Fisher Scientific), followed by the addition of 200 µL chloroform. The samples were then centrifuged at 10,000 g for 15 minutes. The upper phase was mixed with an equal volume of 70% ethanol, and the RNA was further purified using the E.Z.N.A total RNA kit (Omega). RNA-seq was performed by MSKCC genomics core facility using poly-A capture