GSM7659597: Primary Hepatic stellate cells, TILAM LNA 3; Homo sapiens; RNA-Seq
HSCs from donor 4 (Lonza, HUCLS, ID: 201951) were transfected with NTC, PML si17, LNA-CTA, and LNA-TILAM2 in triplicate. Cells were harvested and RNA was extracted using Qiagen RNeasy Mini Kit (74104). RNA quality number (RQN) greater than 9 (Agilent 4200 TapeStation System) underwent polyA-selection and stranded library preparation for sequencing (Genewiz from Azenta Life Sciences). Libraries were prepared with the NEB Directional Ultra II RNA library preparation kit. After library preparation, the libraries were sequenced on the HiSeq platform.