GSM8389206: Hi-C sequencing of CEC4 replicate rep1; Caenorhabditis elegans; Hi-C
Crosslinking of C. elegans embryos and nuclei preparation were done as described by Crane et al. (2015). Frozen embryos were thawed on ice and crosslinked in 50 mL of 2% (v/v) formaldehyde in PBS for 30 minutes at room temperature on a nutator. The crosslinked embryos were washed, dounced, and nuclei were isolated by centrifugation and analyzed for the presence of DAPI-stained nuclei under a fluorescence microscope. Libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® following the manufacturer's protocol. Libraries were sequenced on NovaSeq 6000 PE150 S4 flowcells.