GSM6506682: WT_NI_2 [ATAC-seq]; Homo sapiens; ATAC-seq
Cells were lysed with buffer containing 0.15 NP-40, 0.1% Tween-20 and 0.01% digitonin and incubated on ice for 3 min.  After cell lysis, nuclei were pelleted for 10 min at 4º C in a fixed angle centrifuge. ATAC-seq libraries were prepared using Transposition mix (2X Tagmentation DNA buffer (20 mM Tris-HCl, 10 mM MgCl2, 20% dimethyl formamide), 3.5 µl transposase, 16.5 µl PBS, 0.5 µl 1% digitonin, 0.5 µl 10% Tween-20, 4 µl H2O) made in-house. Libraries were cleanued up individually using Qiagen MinElute PCR purification kits. Transposed DNA libraries were amplified for 4-8 cycles using NEBNext High-Fidelity 2X PCR Master Mix and amplified using unique dual barcoding primers. PCR amplification reactions were purified using MagBio HighPrep PCR clean up kits to remove DNA fragments less than 50 bp and greater than 1000 bp.