GSM7090155: Flt3_WT_1; Mus musculus; RNA-Seq
Mouse bone marrow cells were collected from the femurs, tibiae and iliac crest and depleted of red blood cells by an ammonium chloride lysis step (STEMCELL Technologies). The cells were stained with the fluorophore-conjugated monoclonal antibodies and lineage− c-Kit+ (LK) cells were then isolated using an LSRII Fortessa (BD). Single-cell sequencing libraries were generated using 10x Chromium (10x Genomics, Pleasanton, CA) reagent kit v2 according to the manufacturer's protocol.