rna seq for tomato salt stress leaf 0.5h
RNA-seq library construction was performed using 18 samples from 3 biological replicates of 0, 0.5, 2, 6, 12, and 24 h treated with salt stress, and RNA-seq (RNAseq) was performed on the IlluminaHiSeq2000 platform (Wuhan, China, Maiwei, China). Before constructing the library, we used an Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA, USA) to confirm the quantity and quality of RNA, and the criteria were RNA integrity number >7 and rRNA ratio (28S/18S) >1.5. The extracted RNA was fragmented using PCR plates with magnetic plate holders. Fragment mRNA was reverse transcribed to cDNA using Superscript II and random primers (Invitrogen, Carlsbad, CA, USA). Data filtering and quality control were performed by fastp software23, and the resulting clean data were used for subsequent analysis. Using tomato Heinz 1706 genome (https://solgenomics.net/organism/Solanum_lycopersicum/genome) SL3.0 as a reference, HISAT2 was used for read alignment 24, and String Tie quantified the reads on the alignment 25.