GSM7395265: ASO_L1; Homo sapiens; RNA-Seq
6-FAM (Fluorescein) labeled ASOs were transfected in RSeT cultured ES cells with DT adding, as described above. 48 hours post transfection, single cells were suspended in sorting buffer (HBSS+2% FBS+ 25 mM HEPES) with DAPI, and immediately processed to Fluorescence-activated cell sorting (FACS) at the Sony MA900 machine. 100,000 FAM+ cells (SO-L1 and ASO-L1 transfected, respectively) were sorted to the HBSS+2%FBS buffer and kept on ice during the sorting. The sorted cells were spin-pelleted at 500x g for 4 min and resuspended with 100 ul  HBSS+2%FBS buffer, and then immediately processed on theChromium for barcoding on each sample separately, with the 10x Genomics Single Cell 3' v3 RNA-seq kit and targeting a yield of 12,000 cells. Final libraries were sequenced on a NovaSeq6000 S1 flow cell (Illumina) and achieved for an average depth of >65,000 reads/cell. 10x Genomics Single Cell 3' v3 RNA-seq