WES of liver rep2
DNA samples were extracted from 20-30 mg of liver tissue collected from p53 +/+ mice using a DNeasy Blood and Tissue Kit (#6954; Qiagen) according to the manufacturers protocol. The DNA concentrations in the samples were quantified using a NanoDrop spectrophotometer (Thermo), and all samples with a concentration greater than 50 ug/mL were used for sequencing. All downstream processing, including sequence alignment and variant calling, was carried out at Macrogen Japan. All samples that passed the quality check were used. Library preparation and sequencing were performed using a SureSelect kit (Agilent).