GSM7893390: BE4 [RNA-seq]; Homo sapiens; RNA-Seq
RNAs were harvested by using easy-BLUE™ Total RNA Extraction Kit(iNtRON). Total Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA wassynthesized using random hexamer primers, followed by the second strand cDNA synthesis using dTTP for non-directional library.Then it was followed by end repair, A-tailing, adapter ligation, size selection, amplification, and purification.