RNA-seq of S. cerevisiae: EtOH 1hr treated in Spt5p-AID
Total RNA was extracted from cell pellets using a standard hot-phenol RNA extraction method. DNA was eliminated from the total RNA using a TURBO DNA-free kit (Invitrogen, AM1907) at 37C for 1 h. mRNA was isolated from the total RNA using an NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490) according to the provided protocol. Libraries were prepared using an NEBNext UltraTM II Directional RNA Library Prep Kit for Illumina (NEB, E7760).