GSM7660096: ZH513; Homo sapiens; RNA-Seq
RNA was isolated from fresh frozen tumor tissue (≥ 20 mg) using the AllPrep DNA/RNA Mini Kit (Qiagen). RNA sequencing was performed for all 28 tumors at CeGaT GmbH, Tübingen. Following quality control for RNA integrity, sequencing libraries were prepared from 78 ng RNA using the KAPA RNA HyperPrep Kit with RiboErase (HMR; Roche), whereby ribosomal RNA was depleted. Paired-end sequencing at 100 bp read length was performed on a NovaSeq 6000 sequencing system (Illumina).