GSM6935028: M4 reactivated effector; Mus musculus; RNA-Seq
Sorted cells were frozen in Trizol. Choroform was added and after centrifugation aqueous phase was mixed with 70% Ethanol and isolation was further processed according to MiniElute Kit (Mitenyi). At least 10 ng RNA were used for sequencing RNA preparation was performed with the TruSeq Stranded RNA Kit (Illumina).