GSM7872294: 914_C3; Mus musculus; RNA-Seq
Following electrophysiological recording, single L5 Pyrs were collected immediately by applying light negative pressure through the same glass patch pipette. Following successful aspiration, the sample was transferred in a volume of 4 μL lysis buffer.Collected single cells were immediately spun down at room temperature and put on dry ice until -80°C storage. cDNA libraries were produced using a Smart-seq2-based Single Cell Full Length mRNA-Amplification Kit (N712, Vazyme) according to the manufacturer's instructions. To construct the final sequencing libraries, 1 ng of purified cDNA from each sample was tagmented using an Illumina Nextera XT Library Preparation kit (TD503 Vazyme).