GSM8219898: ATAC LMSU; Homo sapiens; ATAC-seq
ATAC-seq was performed as described in Corces MR et al Nat Methods 2017. Cells were lysed in ATAC-resuspension buffer containing 0.5% NP40, 0.5 % Tween-20 and 0.01% Digitonin. Nuclei were then resuspended in 50 μl transposition mixture containing 25 μl of 2x TD buffer, 16.5 μl of PBS, 5 μl nuclease free water, 0.5 μl of 1% digitonin, 0.5 μl of 10% Tween-20 and 2.5 μl of transposase (Illumina Tagment DNA enzyme 1, #20034198) and incubated at 37°C for 30 minutes with agitation. DNA was extracted using the NEB Monarch® PCR & DNA cleanup kit (#T1030L) and used for PCR using the Illumina/Nextera primers. Library clean-up was performed using Ampure XP beads. ATAC-seq: Libraries were sequenced on a NextSeq platform (Illumina).