Etroplus suratensis genome, HiFi reads, Pacbio Sequel II, Library 3
High molecular weight genomic DNA was isolated from muscle tissue of a single male fish using QIAGEN Genomic-tip 100/G Midi kit (Qiagen, Hilden, Germany). DNA shearing was performed on Megaruptor 3 system (Diagenode, Belgium). Sequencing library was constructed using SMRTbell Express template Preparation Kit 2.0 (Pacific Biosciences, California, USA). The libraries were size selected using BluePippin (Sage Science, USA). About 75 pM of library was loaded onto 8M SMRT cell and sequenced on PacBio Sequel II system in CCS/HiFi mode.