RNA-Seq rectum tumour tissue
Paraffin blocks from the initial tumor diagnosis of all patients were retrieved from the Pathology Departments of both hospitals. Two 4 m-thick sections were obtained from each block and total RNA was isolated with the High Pure FFPET RNA Isolation Kit (Roche). cDNA was obtained and subjected to next generation sequencing (NGS) using Illumina protocols and the HiSeq 1500 platform (Illumina)