Illumina NovaSeq 6000 paired end sequencing; RNA-seq data of ABC from Bm12-induced lupus model
RNA-seq data of ABC from Bm12-induced lupus model
T-bet-tdTomato reporter mice were adoptive transferred of CD4+ T cells from Bm12 mice. After 14 days, ABCs (CD11c+T-bet+CD21- B cell) and non-ABCs (CD11c-T- bet- B cell)  were sorted by FACSAria II. Total RNA was extracted using TRIzol reagent (15596018, Thermo Fisher) Total RNA was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations.