Tn-Seq raw data of Escherichia coli undetermined for
DNA was fragmented (ca. 300 bp) and P5 Illumina adapters were linked to the fragmented DNA. Junction fragments on both sides of EZ-Tn5 were amplified using standard P5 Illumina primer in association with Tn5-hybridizing primer HV1 or HV2 carrying the P7 Illumina adapter on the 5 end