GSM8305619: Heart, HFD, novirus, rep4; Mus musculus; RNA-Seq
Total RNA was isolated using the RNeasy Mini Kit (QIAGEN) and the RNase-Free DNase Set (QIAGEN) according to the manufacturer's instructions. cDNA was synthesized using the Affinity Script cDNA Synthesis Kit (Agilent Technologies) with 0.25 μg total RNA. RNA library preparations and sequencing reactions were conducted at GENEWIZ, LLC. (South Plainfield, NJ), per their standard operating procedure