GSM7978086: AT2-4_CONTROL_R2; Homo sapiens; RNA-Seq
Total RNA was extracted from each of the pre- and post- SARS-CoV-2 infection challenged AT2s (2 replicates each of the SARS-CoV-2 infected, and vehicle-treated cells per sample) using a commercially available RNeasy Mini Kit (Qiagen, Germantown, MD, USA) and the manufacturer's protocol. RNA quality and quantity were assessed using a NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and an Agilent 2200 TapeStation system (Agilent, Santa Clara, CA, USA). 1 µg of high-quality total RNA per sample per replicate and the reagents supplied in Illumina Stranded mRNA sample preparation kit v2 (Illumina, Inc., San Diego, CA, USA) were used to prepare mRNA sequencing libraries.