GSM8048858: e16smo_cy_cntrl_yz060; Mus musculus; OTHER
Rosa26SmoM2/+ (smo_cn), and hGFAP-Cre; Rosa26SmoM2/+ cortices (smo_cy) (n=3 each group) were dissected, and cells were isolated with the Accumax (Sigma-Aldrich A7089) following the manufacturer's instructions. For CUT&RUN, H3K27ac, H3K4me3, H3K27me3, and IgG (cntrl) antibodies were used. 10,000 cells were used for each experiment. For CUT&RUN, DNA libraries were constructed using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645, E7103) with index primers (NEB, E7645-S).