Kurthia sp. 11kri321 complete genome sequencing and assembly
The Illumina NextSeq 2000 platform performed whole genome sequencing of one bacterial DNA sample, generating paired-end reads with a read length of 151 bp. We used FastQC analysis (Andrews, 2010) to check the quality of the data, and then we used Fastp v0.23.4 with the parameters --average_qual 20 and --length_required 35 to trim the adapter and filter the quality. We performed a de-novo genome assembly from the trimmed reads using Unicycler v0.5.0, setting a minimum contig length of 200 bp (Wick et al., 2017). Finally, we conducted genome annotation using Prokka v1.14.5, adhering to genetic code 11 (Seemann et al., 2014).