Illumina NovaSeq 6000 paired end sequencing; Single-cell RNA-seq of human breast tumor samples with exhausted and non-exhausted immune environments
Single-cell RNA-seq of human breast tumor samples with exhausted and non-exhausted immune environments
Fresh human breast tumor tissue was dissociated into single cells as previously described (Wagner et al., Cell 2019) 10x v3 protocol; Chromium Single Cell 3สน GEM, Library & Gel Bead Kit v3, 16 rxns PN-1000075 10x v3 protocol; Chromium Single Cell 3สน GEM, Library & Gel Bead Kit v3, 16 rxns PN-1000075