GSM7871739: Control (after induction) rep1; Mus musculus; RNA-Seq
Cells were collected using TrypLE Express Enzyme, lysed in RLT buffer (QIAGEN, Catalog No. 74004), and the RNA was extracted using the QIAGEN RNeasy Micro Kit (QIAGEN, Catalog No. 74004) according to the manufacturer's instructions. Purified RNA was quality checked using a TapeStation 4200 (Agilent), and 500 ng were used for QuantSeq 3′ mRNA-Seq library construction according to the manufacturer's instructions (Lexogen, Catalog No. 015.96).