IgM reverse primer selected RNA-seq of mus musculus: splenocytes
RNeasy Mini Kit (Qiagen) -> RT-PCR using SuperScript IV with random primers -> PCR round 1 with P5-H10-leader paired to P7 + IgM reverse primers -> QIAquick Gel Extraction Kit (Qiagen) -> Illumina Miseq kit V3 (2 x 300 bp; Illumina) cartridges