16S rRNA of sorghum roots treated with dSynCom -exudate solubilizers
Samples were amplified using a dual-indexed 16s rRNA Illumina iTags primer set specific to the V3-V4 region 341F (5-CCTACGGGNBGCASCAG-3) and 785R (5-GACTACNVGGGTATCTAATCC-3) using Invitrogen Platinum Hot Start PCR Master Mix (2X), (Catalog No. 13000014, Waltham, Massachusetts) and 10 ng of template per reaction.