GSM7927551: sgTIGRE-tumor 1; Mus musculus; RNA-Seq
RNA was extracted using the Trizol reagent (invitrogen) and cleaned using the Qiagen RNeasy kit. 50 ng per sample was used for library preparation with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina after the total RNA was depleted using NEBNExt rRNA depletion kit