GSM7854647: RAO55 xenograft, Enzastaurin; Homo sapiens; ATAC-seq
Flash-frozen tissues were sent to Active Motif to perform the ATAC-seq assay. The tissues were manually disassociated, isolated nuclei were quantified using a hemocytometer, and 100,000 nuclei were tagmented as previously described (Buenrostro et al. 2013), with some modifications based on (Corces et al. 2017) using the enzyme and buffer provided in the ATAC-Seq Kit (Active Motif). Tagmented DNA was then purified using the MinElute PCR purification kit (Qiagen), amplified with 10 cycles of PCR, and purified using Agencourt AMPure SPRI beads (Beckman Coulter). Resulting material was quantified by Nanodrop and sequenced with PE42 sequencing on the NovaSeq 6000 sequencer (Illumina).