16S rRNA Sequencing of Amynthas gracilis: gut content, farming soil with good practices (GP), rainy season
The DNeasy PowerLyzer PowerSoil Kit (Qiagen Corp.) was used for extracting total microbial DNA from the gut content, following the manufacturer's specifications. The DNA concentration, quality and integrity were verified through gel electrophoresis and NanoDrop 2000 (Thermo Fisher Scientific). The V4 region from the 16S rRNA gene was PCR amplified using the primers 515F (5- GTGCCAGCMGCCGCGGTAA-3) y 806R (5-GGACTACHVGGGTWTCTAAT-3). The amplicons were used for library preparation and sequencing with the Illumina Novaseq 6000 system 2 x 250 bp (Novogene Inc.).