GSM8305728: Western diet alcohol, saline injections, replicate 2, RNAseq; Mus musculus; RNA-Seq
Mouse livers were dissociated by perfusion and collagenase digestion and total non-parenchymal cells were isolated by differential centrifugation. Non parenchymal cells were further purified by FACS and live CD45 positive cells were used for the single cell RNAseq protocol. RNA libraries were prepared for sequencing using standard Illumina protocols