GSM8269296: RUES02, primed (E8), scRNAseq; Homo sapiens; RNA-Seq
RUES02 hESC were prepared in E8 (primed) or LIF-3i/MEF (TIRN; TIRN +iDUX4 after 24 hours supplementation with 2mg/mL doxycycline). Cells were gently collected using a mixture 1:1 Versene/Accutase (5 min) and pass through a 70um cell strainer. Viable cells were enriched using the MACS Dead Cell Removal Kit (Miltenyi Biotec) and prepared in PBS, 0.4% BSA. Cell quality check (cell viability >95%, cell diameter) was verified before preparing libraries. Library preparation was performed according to the manufacturter's instructions (Chromium Next GEM Single Cell 3' Gene expression).