GSM8178175: sample_Relapse_2_scMultiomics_ATAC; Homo sapiens; ATAC-seq
Sorted B-cells were subjected to nuclei isolation protocol (10X).The nuclei were immediately processed with 10x Genomics Chromium controller using Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Kit. The libraries were generated according to the Chromium Next GEM Single Cell Multiome ATAC + Gene Expression protocol and the quality was checked using Agilent High Sensitivity DNA 1000 Kit on Agilent Tape Station. Libraries were quantified using dsDNA High-Sensitivity (HS) Assay Kit (Invitrogen) on Qubit fluorometer and the qPCR-based KAPA quantification kit. RNA libraries were sequenced on an Illumina Nova-Seq 6000 with 18.10.10.90 paired-end format and ATAC libraries with 50.8.24.49 paired-end format.