Microbial 16S of human colostrum
Colostrum samples from mothers with normal BMI (n=20) and with obesity (n=28) were collected. DNA samples were subjected to Zymo Research's Quick-16S kit, utilizing primers targeting the V3-V4 regions of the 16S rDNA. The sequencing was performed on a V3 MiSeq 622cyc flowcell, generating 2x301 bp paired-end reads. Amplicon sequence variants (ASVs) were analyzed with the QIIME2 software. Alpha and beta diversities were compared between clinical settings, and differential abundant taxa was evaluated.