GSM8305601: PC3 cell Rep2; Homo sapiens; ncRNA-Seq
EV RNA isolation in this study was conducted using TRIzol-choloroform method followed by isopropanol precipitation and purification using Qiagen exoRNeasy Midi Kit, following the manufacture's protocol. For ATPS isolated EVs after dextranase treatment, ratio between the volume of EV samples, TRIzol (Thermo Fisher Scientific) and choloroform (Fisher Scientific) was 1:5:1. Top aqueouse layer after TRIzol-choloroform separation was mixed with isopropanol (Sigma-Aldrich), 1:1 in volume. RNA was precipitated overnight at -20 °C. For UC isolated EVs, EV pellets were directly mixed with TRIzol. RNA isolation from human cells were conducted using the same TRIzol method. Single-end Small RNA sequencing libraries were prepared using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® kit (NEB#E7560S), following manufacture's instruction.