GSM7050256: WM2_uninf; Mus musculus; RNA-Seq
At 6 days post PR8 infection animals were sacrificed and perfused with 10ml cold PBS. Lungs were collected and single cell suspensions were prepared using the Miltenyi Biotec Lung Dissociation Kit (130-095-927) with the gentleMACS Octo Dissociator (130-095-937) following the manufacturer instructions. Cells were loaded onto the Chromium Controller (10x Genomics) and single-cell RNA-seq libraries were prepared using the Chromium Single Cell 3ʹ Reagent Kits v3 (10x Genomics) following the standard manufacturer protocols.