GSM8174721: Normal_ATAC_3; Mus musculus; ATAC-seq
A total of 200,00 RPE cells were lysed in cold lysis buffer (10 mM NaCl, 10 mM Tris-HCl pH 7.4, 3 mM MgCl2, 0.1% (v/v) IGPAL CA-630,) for 20 min on ice. The nuclei were resuspend with reaction buffer (10 μl l TruePrep Tagment Buffer L, 3 μl TruePrep Tagment Enzyme, and 35 μl ddH2O from Vazyme, TD501) at 37°C for 30 min. The cells were subjected to transposition with Tn5 transposase (TD501, Vazyme) for 20 min at 37°C. DNA was purified with QIAquick PCR Purification Kit (28106, QIAGEN) for final library construction.