16S metagenomics of cattle rumen microbiome: sorted using FACS
DNA was extracted using the AllPrep PowerFecal DNA/RNA kit (Qiagen, Canada). The V4 region of 16S rRNA was targeted for amplification by PBR using the forward primer 515F and reverse primer 806R and CS2 tags were used to add a barcode and Illumina adapters. Amplification was performed using Q5 High Fidelity DNA polymerase with PCR cycles.