GSM6935542: Patient3_Post_WES; Homo sapiens; OTHER
Total RNA was extracted using RNeasy kit (Qiagen) followed by on-column DNase I digestion according to the manufacturer's protocol. Genomic DNAs were purified from FFPE samples using AllPrep DNA/RNA FFPE kit (Qiagen). Sequencing libraries were prepared using the KAPA RNA HyperPrep Kit with RiboErase (HMR) (Roche, Cat# 08098140702). Whole exome sequencing libraries were constructed with Sure Select Human All Exon v6 (Agilent) and the Sure Select XT2 library prep kits (Agilent). Total RNA-seq were sequenced as 50 bp paired-end. WES were sequenced as 75 bp or 150 bp paired-end