RNAseq of rat peritoneal macrophage: replicate 1 of macrophages with LPS treatment and FIR irradiation
Rat macrophages were completely lysed by 1 mL RNAiso (TAKARA, NO9109), then the total RNA was purified using RNAsimple Total RNA kit (TIANGEN, DP419). The quality of purified RNA was examined by an Agilent 2100 bioanalyzer. Next, NEB Next Ultra RNA Library Prep Kit for Illumina (NEB Next, E7530L) was used for library preparation. Finally, we pooled high-quality samples with product sizes ranging 300500 bp in equimolar concentrations, then submitted for 150 bp paired-read sequencing on the Illumina HiSeq X-ten.