GSM7927093: S17_RBMX_TSS; Homo sapiens; OTHER
4C-seq libraries were prepared following the published protocol (van de Werken et al., 2012). For the GPR101 viewpoint, Csp6 and DpnII were used for the first and second digestion, repsectively. For RBMX and VGLL1 viewpoints, DpnII and Csp6 were used for the first and second digestion, repsectively. Parallel PCR reactions were performed to amplify from a total of 1 µg template per 4C library and viewpoint. PCR reactions were pooled and purified using 1.8x volume of AMPure XP beads (Agencourt, A63881). Samples were indexed and sequenced with Ilumina Hi-Seq technology according to standard procedures.