Fungal ITS2 of roots from olive trees with pest
Metagenomic analyses of the fungal ITS2 region were performed on the primers ITS4 (5' TCCTCCGCTTATTGATATGC 3')and ITS2_fITS7 (5 GTGARTCATCGAATCTTTG 3) . To prepare libraries for Illumina sequencing, primers ITS4 and ITS2_fITS7 were fused with the Illumina adapter overhang nucleotide sequences. For each library, root PCR products were sequenced on the Illumina MiSeq platform using a 2300 nucleotide paired-end protocol.