GSM6896374: PD3; Mus musculus; RNA-Seq
Total RNA was isolated from tumor tissues using TRIzol (Invitrogen) following the manufacturer's protocol. The RNA quality was assessed using a Bioanalyzer 2100 System (Agilent Technologies, Santa Clara, CA, USA). RNA concentration was measured using a NanoDrop ND-2000 (Thermo Fisher Scientific). The cDNA libraries were constructed using the QuantSeq 3′ mRNA-Seq Library Prep Kit (Lexogen, Inc., Vienna, Austria) and sequenced on a NextSeq 500 (Illumina Inc., San Diego, CA, USA) to generate single-end 75 bp reads according to the manufacturer's instructions.