scATAC of Human: villous
Villous leukocytes (n=4) were thawed, and surface stained with CD14, HLA-DR, FOLR2, CD9, CCR2 and Sytox Green then sorted on a BD FACSAria Fusion. Nuclei were isolated from each subset, counted, and transposition reaction performed according to manufacturers instructions (10X Genomics). ~10,000 nuclei were obtained per sample, except for PAMM2 where ~1000 nuclei were obtained and loaded into the 10X Chromium Controller. Library preparation (10X v2 chemistry) was performed for samples according to manufacturer protocol and sequenced on a NovaSeq S4 (Illumina) to a depth of >25,000 paired reads/cell.