GSM8002580: Human Uterine Leiomyosarcoma, C10/C12; Homo sapiens; RNA-Seq
Formalin-fixed paraffin-embedded block was sectioned at 50um thickness, digested to single-cell suspension with the Qiagen FFPEasy kit, and filtered with 30um cell strainers. Cell suspension was hybridized to the 10X Genomics Fixed RNA Profiling human transcriptome probeset. Sample was tagged with Human Probe Barcodes BC003 and BC004 (10X p/n 2000497 and 2000498) prior to pooling. After probe and barcode hybridization, suspension was partitioned into single cells according to manufacturer instructions. Library was constructed according to manufacturer protocols.