GSM8386409: colon, D21_AOM_48h_7; Mus musculus; RNA-Seq
Total RNA was isolated using TRIzol™ Plus RNA Purification Kit (Thermo Fisher Scientific) from frozen colon tissue after pulverization in liquid nitrogen with mortar and pestle. The integrity of the total RNA was determined using a Tapestation (Agilent) and concentration were assessed by Qubit (ThermoFisher Scientific).  Libraries were prepared using the TruSeq Stranded Total RNA kit (Illumina, San Diego, CA) with Ribo-Zero technology. The indexed samples were sequenced on the Nova-seq 6000 (Illumina) employing a 75-bp single-end protocol