GSM6895354: LIRIL2R_input_rep2; Homo sapiens; ChIP-Seq
A total of 19 Chromatin Isolations via RNA Precipitation (ChIRP) probes tiling LIRIL2R were designed at BIOSEARCH TECHNOLOGIES. The probes were serially numbered, based on their position across the transcript. Even and odd probes were then pooled to ensure specificity of the pull-down. The published ChIRP protocol was used for the RNA pull down (Chu et al. 2012) following the instructions of the kit (EZ-Magna ChIRP RNA Interactome Kit, Cat# 17-10495, Merck Millipore). A modified ChIP-seq protocol and illumina ChIP-seq TrueSeq kit were used for preparing the libraries, which were sequenced (2x100bp; 40 million reads/library) on an illumina NovaSeq 6000 instrument.