GSM7926868: Muller glia from mych-/- retinas 2 days post injury (dpi) rep1 [5803-ML-7]; Danio rerio; RNA-Seq
Retinas from gfap:GFP transgenic zebrafish were dissected from fish eyes, tissue was dissociated using Papain Dissociation system (Worthington Biochemical) and GFP+ Muller glia isolated by FACS. RNA was purified using Directzol RNA microprep kit (Zymo Research). Illumina RNA (PolyA-enrichment) library prep kit was used according to manufacturer's directions.