GSM8306067: H128-H10-2; Homo sapiens; ATAC-seq
sample 1-2: ATAC sequencing service was provided by CloudSeq Biotech (Shanghai, China) with GenSeq® ATAC kit (GenSeq Inc.). Briefly, cells were lysed using cold lysis buffer. After lysis, nuclei were collected by centrifuging at 500 g for 10 min at 4℃. The pellet was transposed with transposome and add tags on the both sides of fragmented genomic DNA according to the manufacturer' s instruction. After tagmentation, the purified tagged DNA was used for PCR amplification with Genseq® 2× HiFi PCR Mix (GenSeq Inc.). The libraries were quantified using Qubit fluorometric assay (ThermoFisher) and then sequenced. in a NovaSeq platform (Illumina). sample 1-2: ATAC sequencing service was provided by CloudSeq Biotech (Shanghai, China) with GenSeq® ATAC kit (GenSeq Inc.).