nasal metagenome of Bos taurus
Sequencing libraries were constructed using 100-500 ng of gDNA using the Illumina DNA Prep sequencing library kit (Illumina, San Diego, CA). The sequencing library construction included tagmentation of the gDNA using a bead-based transposome complex to simultaneously fragment and tag the DNA with adapter sequences.  Following tagmentation, unique dual index adapters are added in a PCR amplification step to the ends of the DNA fragments.