Ion Torrent S5 XL sequencing; Differential expression of genes in whole brain comparing SLC38A10 knock out and wild type mice
Differential expression of genes in whole brain comparing SLC38A10 knock out and wild type mice
Whole mouse brains were dissected and rapidly frozen on dry ice. Whole brains were homogenized using a bullet blender and glass beads in 10 ml Tris buffer. Whole brains were homogenized using a bullet blender and glass beads in 10 ml Tris buffer. 600 ul of the homogenate was used for RNA extraction using the Qiagen RNAEasy mini kit according to the manufactories recommendations. The library was constructed using the Ion AmpliSeq™ Library Kit from Thermo Fisher. For this the Ion AmpliSeq™ Transcriptome Mouse Gene Expression Kit primer panel from Thermo Fisher was used. All procedures wrer performed according to the recommended protocol.