WGS of SNU16m1 pemigatinib day 42 replicate 1
WGS libraries were prepared by DNA tagmentation. We first transposed it with Tn5 transposase produced as previously described57, in a 50-l reaction with TD buffer58, 50 ng DNA and 1 l transposase. The reaction was performed at 50C for 5 minutes, and transposed DNA was purified using MinElute PCR Purification Kit (Qiagen, 28006). Libraries were generated by 5-7 rounds of PCR amplification using NEBNext High-Fidelity 2 PCR Master Mix (NEB, M0541L), purified using SPRIselect reagent kit (Beckman Coulter, B23317) with double size selection (0.8 right, 1.2 left).