GSM7180235: Control 6850-XZ-1; Mus musculus; RNA-Seq
Using the RNeasy Mini Kit (Cat. 74104, Qiagen), we isolated the total RNA, according to the manufacturer's instructions. DNase I treatment (Cat.79254, Qiagen) was performed during the RNA isolation procedure to remove genomic DNA, following the manufacturer's recommendation. Total RNA was quantified using the NanoDrop One (Thermo Scientific, USA) and Agilent Tape Station. Poly-A (standard) Library Prep. NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490) and xGen Broad-range RNA Library Prep (IDT, 1009813) with xGen Normalase UDI Primers (IDT, various)