GSM7872419: H3K9me2, lsd2 clr4 del, rep2; Schizosaccharomyces pombe; ChIP-Seq
Whole cell extracts were incubated with 50 units of AcTEV protease (Invitrogen Corp. Catalog#: 12575015, Massachusetts, USA) and 50µL IgG Sepharose (GE Healthcare, Catalog#: 17096901, Illinois, USA) at 4 °C overnight. The next morning, pre-cleared whole cell extracts were collected after centrifugation at 17,530 × g for 10 min, followed by adding the antibody (Anti-myc, Abcam ab9132). Reverse cross-linking and elution were performed at 65o C overnight. ChIP DNA was purified using DNA purification columns (Thermo Fisher, K0512), and quantified by quantitative-PCR (QuantStudio 3, Applied Biosystems) using SYBR Select Master Mix (Applied Biosystems, 4472908).