GSM6935401: 153 - 3m_WT_rep2; Mus musculus; RNA-Seq
TRIzol® Reagent, Life Technologies, according to manufacturer's instruction. the poly(A) fraction (mRNA) was purified from 500 ng of total input RNA followed by fragmentation and the generation of double-stranded cDNA. After Agencourt Ampure XP beads cleanup (Beckman Coulter), end repair, A base addition, adapter ligation, and PCR amplification steps were performed. Libraries were quantified by Qubit (Thermo Fisher Scientific) and TapeStation (Agilent).