GSM8048457: totalRNAseq for mESC F123 Replicate1; Mus musculus; RNA-Seq
Total RNA was extracted from F123 mESCs using TRIzol Reagent (Invitrogen, Cat# 15596026) following manufacturer's instructions. Total RNA was analyzed on the Bioanalyzer using the Agilent RNA 6000 Nano Kit to ensure intact, non-degraded RNA presence and was subsequently treated with TURBO DNase I (Ambion, Cat# AM1907). Total RNA-seq libraries were generated from 1 μg of DNase-treated RNA using the TruSeq Stranded total RNA library preparation kit (Illumina, Cat# 15031048) according to the manufacturer's instructions. Samples were pooled and sequenced on the NextSeq500, paired end 75 bp, following the manufacturer's instructions.