Healthy_cfDNA_br2
Sequencing libraries for regular cell-free DNA were prepared with the NEBNext Ultra II DNA Library Prep Kit (NEB) according to the manufacturers protocol. 0.5 ng isolated cfDNA was used as input. The NEBNext Adaptor was diluted 1:25 for all reactions. PCR was performed with 10 PCR cycles and 4 L primers. Sequencing was performed on a NextSeq 2000 (Illumina) with 100 bp single end reagent kits.