CRISPR-CATCH of SNU16m1 ecDNA: 39
Target DNA molecules were purified using CRISPR-CATCH. To perform short-read sequencing, we first transposed it with Tn5 transposase produced as previously described28, in a 50 ul reaction with TD buffer29, 10 ng DNA and 1 ul transposase. The reaction was performed at 37C for 5 minutes, and transposed DNA was purified using MinElute PCR Purification Kit (Qiagen, 28006). Libraries were generated by 7 rounds of PCR amplification using NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541L), purified using SPRIselect reagent kit (1.2X, Beckman Coulter, B23317)