GSM6507181: NAcc sample from mouse 33 [S33_NAcc]; Mus musculus; RNA-Seq
RNA were extracted from each brain region using the QIASymphony robot and standard Qiagen kits/reagents (Qiagen Sciences Inc, Germantown, MD) following the manufacturer's recommendations. Brain tissue were obtained from 1-mm coronal slices by hand under RNAse-free conditions. (Gene Profiling Shared Resource, Oregon Health & Science University) RNA libraries were prepared with a TruSeq stranded mRNA library prep Kit (cat# RS-122-2101, Illumina, San Diego, CA, USA). RNA-Seq: The libraries were sequenced on a Illumina NovaSeq 6000 (Massively Parallel Sequencing Shared Resource, Oregon Health & Science University) using a paried-end read.