GSM8269457: control diet, male, biol rep 1; Mus musculus; RNA-Seq
Mice were anesthetized with ketamine/xylazine and decapitated. Brains were removed, meninges were peeled off, and cerebellum and olfactory bulbs were discarded.  A series of enzymatic and mechanical steps was used to achieve a single cell suspension of the remaining brain tissue. FACS was used to enrich for CD31+ endothelial cells, which were sorted into trizol for RNA extraction. Total RNA was extracted using the RNeasy Micro kit from QIAGEN. Non-stranded mRNA libraries were made using Illumina's TruSeq RNA Library Prep Kit v2 according to manufacturer's instructions