Fecal microbiota from the germfree mouse (ID 2343) 21 days after receiving the fecal microbiota from wild mice, and was treated with control antibody, day 12
Amplicons of the V4 region within the 16S rRNA gene were generated and both ends of the fragments were sequenced. The paired end sequences were assembled into 250 bp contigs, aligned to the SILVA 16S rRNA database release 138, made free of possible chimeric sequences by UCHIME, binned into Operational taxonomic units at >97 % identity level and taxonomically assigned with 16S rRNA gene training set version 16 of Ribosomal Database Project.