GSM7872314: S169 96h; Homo sapiens; RNA-Seq
RNA was purified using QIAGEN RNeasy AllPrep kits according to manufacturer's instructions. Libraries were constructed using Illumina Nextera Library Prep Kit with i7 indexes. Concentrations were measured using Qubit HS DNA Assay (Thermo Fisher Scientific, Paisley, United Kingdom). Library sizes were assessed on an Agilent Technologies 2100 Bioanalyzer before sequencing on the Illumina Nextseq platform paired-end reads (42 bp (R1) and 42 bp (R2)). One cartridge was used per 2 patients i.e. 20 multiplexed samples per cartridge.