GSM7870521: sample31_4UE_48h; Homo sapiens; RNA-Seq
RNA was isolated from PAXgene blood RNA tubes using Qiagen's BloodRNA Kit following manufacturer's protocol. Samples were sent to BGI Genomics (Danmark branch) for library construction and sequencing. mRNA was enriched using oligo dT beads, and then fragemented. Fragmented RNA was reverse transcribed to double-strand cDNA using random primers.Synthesized cDNA was subjected to end-repair and 3' adenylated. Adaptors were ligated to the ends of these fragements before PCR amplification and purification. After quality control and library circulization, the library was amplified to make DNA nanoball (DNB). Finally, sequencing was conducted using the DNBSEQ platform.