GSM6621763: Apoe-/-, replicate 1, scRNAseq; Mus musculus; RNA-Seq
Peritoneal cells were isolated from C57BL/6 mice or Apoe−/− mice which were injected with 10 ml of cold PBS into the mice peritoneal cavity. Cells were washed and re-suspended in PBS. The cell suspensions obtained individually from the peritoneal cavity were passed through a 100 µm cell strainer and were stained with anti-mouse F4/80-FITC for 30 min. The total viable F4/80+ cells were selected using Beckman Coulter's MoFlo Astrios EQ for scRNA-sequencing. RNA libraries were generated by 10xGenomics Single Cell 3' Library Gel Bead Kit V3 and prepared for sequencing using standard Illumina protocols