GSM7393984: RSeT media Rep1, SO-L1; Homo sapiens; RNA-Seq
RNA extraction was performed using the RNeasy mini kit (QIAGEN), according to the manufacturer's instructions. 80-130ng equal volume RNA each sample was used to prepare sequencing library. The cDNA library was prepared with NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #  E7490S) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB # E7760L), following the manual instruction. Library cDNA were equally pooled at 10 nM for sequencing.