scRNA-Seq of mus musculus: retina
The cells were processed into barcoded scRNA-seq libraries using the Chromium Single Cell Library, Gel Bead & Multiplexing Kit (10x Genomics), following the guidelines provided by the manufacturer. In summary, the cells were partitioned into gel beads in an emulsion using the ChromiumTM Controller instrument, followed by cell lysis and reverse transcription of RNA barcodes. The libraries were prepared using the 10x Genomics Chromium Single Cell 3 Library & Gel Bead Kit v3.1 according to the manufacturers prescribed protocol. Sequencing was then performed using an Illumina Nova6000 sequencer to obtain 150 bp paired-end reads.