GSM7893023: PND0-WT3; Mus musculus; RNA-Seq
RNA was isolated using the Qiagen Lipid RNeasy Kit following the manufacturer's protocol. Samples were sent to Novogene Co. Ltd (Sacramento, CA) for total RNA sequencing on their Illumina platform. RNA sample quality control was further assessed, and the mRNA library was prepared with additional polyA enrichment, RNA fragmentation, and cDNA transcription steps. Libraries were sequenced by 150 bp paired-end reads on the Illumina NovaSeq 6000 Sequencing System with a minimum sequencing read depth of 6 Gb of raw data per sample.