ITS-Amplicon of honey metagenome: Melipona mondury replicate 2
20 mL of honey was diluted with 180 mL sterile distilled water (1:10) to minimize the amount of carbohydrates, and filtered (using 22 m filter) to retain microbial cells. Then, we cut the filter and were pieces vortexed in Phosphate Buffered Saline (PBS) to resuspend bound microbial cells. Total DNA was extracted and sequencing was performed at MR DNA (Shallowater, TX, USA) on an Illumina 165 MiSeq 2x300 bp paired-ends.