GSM7016920: NM1 WT bone marrow 03; Mus musculus; RNA-Seq
Bone marrow was isolated according to the previously published protocol (Amend et al., 2016). In short, femurs and tibiae were taken from euthanized mice and cleaned from surrounding muscles, connective tissues and epiphysis parts of each long bone. All bones from single mice were placed in the 0.5ml tube with the hole on the bottom nested in the 1.5ml collection tube. Bones were spin down at 14000 x g in a microcentrifuge for 15 seconds to pellet the bone marrow at the bottom of the bigger tube. For each bone marrow sample, femurs and tibiae from single NM1 WT or KO mice were used. RNA was isolated by RNAzol according to manufacturer's protocol and final RNA concentration measured by Qubit RNA BR Assay Kit. Three replicates were prepared for each genotype. 500ng of total RNA was used for the RNA-Seq library preparation by using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB) and sequenced with the NextSeq 500/550 sequencing platform (performed at the NYUAD Sequencing Center).