GSM6894192: BT549-si-3; Homo sapiens; RNA-Seq
RNA was harvested using trizol. 1.3 ug of total RNA was used for the construction of sequencing libraries. After the RNA sample is tested as qualified, the preparation of the entire library is completed through mRNA enrichment, fragmentation, cDNA one strand synthesis, two strand synthesis, end repair, adding A tail, adding sequencing connector, purification, PCR amplification and other steps.