GSM7016960: YS005; Mus musculus; RNA-Seq
After euthanising the mice according to animal protocol, we collected the liver and make it into pieaces for analysis. RNA was isolated using Roche High pure RNA extraction kit or QIAGEN RNeasy mini kit accoring to the manual protocol. We use poly-A pull-down to enrich mRNAs from total RNA samples, then proceed with library construction using Illumina TruSeq chemistry. Libraries are then sequenced using Illumina NovaSeq 6000 at Columbia Genome Center. We multiplex samples in each lane, which yields targeted number of paired-end 100bp reads for each sample.