GSM6731338: N2A_H3K9me3_hemin; Mus musculus; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using VCX150 (Sonics). 5ng of purified ChIP DNA was used to generate the sequencing library using a NEB kit and sequenced with the Illumina HiSeq X Ten. DNA-end repair, 3'-dA overhang and ligation of methylated sequencing adaptors use Paired-End DNA Sample Prep kit (Illumina). PCR amplification was size selected at 100-300bp. Qualified library for sequencing.