GSM8048353: Tshr_ko_retina_sup_1; Mus musculus; RNA-Seq
For single cell analyses, cones labeled with a Thrb2Cre driver were dissociated from superior and inferior pieces of retina of Mct8-KO and control male mice at 2 months of age. Cones were isolated individually by micromanipulator to minimize contamination by rods, then examined visually for cone morphology and integrity before library construction. For dissected retina and isolated RPE cells, all samples were taken from superior and inferior areas around the vertical mid-line of the eye, then incubated in RNAprotect Cell reagent before RNA extraction with Qiagen RNeasy kit. Single cell RNA-seq library library construction with SMART-Seq v4 Ultra Low Input RNA kit and ThruPLEX DNA-Seq Kit, purified RNA from different retina and RPE samples were constructed into RNA-seq libraries with SMARTer stranded total RNA sample prep kit and ThruPLEX DNA-Seq Kit.