GSM8178278: B16F10_15dpi_tumor; Mus musculus; RNA-Seq
The mRNA of AAVS1-KO and CALHM2-KO primary NK cells with or with cancer stimulations were extracted with RNeasy plus kit (Qiagen) according to manufacturer's instruction. NK cells were normalized to 1,000 cells / μL. Standard volumes of cell suspension were used for 10X Genomics gene expression library preparation to achieve a targeted cell recovery of 10,000 cells.