GSM7659154: exp4_ch11_TCR; Homo sapiens; OTHER
Isolated blood T cells were left unstimulated or stimulated for 20 h with MR1/5-OP-RU (TCR), IL-12 + IL-18 (cytokine) or both, before MAIT cell (CD3+CD26+CD161hiVα7.2+) sorting. Additionally, isolated T cells were left unstimulated or stimulated for 68 h with both MR1/5-OP-RU and IL-12 + IL-18 (TCR+cytokine), before MAIT cell sorting. Single-cell gene, surface protein and TCR repertoire analysis was performed using 10x Genomics technology. Sequencing libraries were generated using the 10x Genomics Chromium Next GEM Single Cell 5' Reagent Kits v2 (Dual Index).