GSM6732152: TCR-CD8_WT_Liv; Mus musculus; RNA-Seq
Single-cell suspension from splenocytes and liver T cells from three C57BL/6 mice and three EomesTg/Tg mice were obtained as described. Cells from each strain were pooled and CD8+ T cells from spleens and livers were sorted. Microscope assessment after Trypan blue staining allowed to count viable cells following FACS sorting. Cell preparations were centrifuged at 500 RCF for 7 min and pellets were resuspended in 10%FCS/RPMI medium. Cells were loaded on the Chromium Controller (10x Genomics) Single-cell RNA-seq libraries were prepared using the Chromium Single Cell 5′ v1 Reagent Kit (10x Genomics) according to manufacturer's protocol.