UBC-GFP-zebra-finch-genome
Genomic DNA was obtained from blood. The medial metatarsal vein in the leg of a male GFP+ zebra finch was nicked with a sterile 20 gauge needle. Blood was drawn with a glass Micro-Hematocrit capillary tube (Thomas Scientific). DNA was then extracted from 10 l of blood using a genomic DNA extraction and purification kit (New England Biolabs). The sample was sent overnight on dry ice to a commercial next-generation sequencing company (LC Sciences, Houston, TX) for genome sequencing with Illumina. The following processing and analysis steps were performed by LC Sciences: 1) Adapters and low quality reads were removed by Cutadapt and FastP softwares, respectively. 2) Data quality control was performed using the FastQC software. For genome alignment, BWA MEM software was used. 3) Alignment of sequenced genomes was performed to determine the insertion site of the transgene. 4) Genome alignment to plasmid and reference genome was viewed in IGV, and allowed for insertion sites to be determined.