GSM6896638: Venom gland, milked, 4 days before dissection, rep1; Androctonus amoreuxi; RNA-Seq
Total RNA was extracted in TRIzol (Thermo Scientific, UK) and purified on RNeasy micro columns with on column DNase digestion (Qiagen, CA), according to the manufacturer's instructions. Total RNA was quantified by fluorimetry (Qubit, Thermo Scientific, UK) and quality (RIN 9.1) was assessed on a Tapestation 4200 (Agilent, CA). Unique dual indexed stranded TruSeq mRNAseq libraries were prepared from 500 ng total RNA, according to the manufacturer's instructions (Illumina, CA). Libraries were quantified by qPCR with SYBR green (Kapa Library Quantification Complete Universal, Roche, CH) on the QuantStudio 6 Flex (ThermoFisher Scientific, UK) and average library size of 291 bp determined on the Tapestation 4200 (Agilent, CA) prior to sequencing and base calling on an Illumina NextSeq500 with v2.5 chemistry and 75 bp paired-end reads, with 14.3 Gb sequence output