RNA-Seq of Megalobrama amblycephala X Gobiocypris rarus liver
The first and second strands of cDNA were synthesized using a random hexamer primer, M-MuLV Reverse Transcriptase, and dNTPs. cDNA fragments 150~200 bp in length were collected and purified with an AMPure XP system (Beckman Coulter). Then, the short fragments were used to perform PCR amplification and construct libraries.