GSM6774693: Elys-KD RNA-seq, rep 1; Drosophila melanogaster; RNA-Seq
Approximately 107 control and Elys-KD S2 cells were collected. Poly(A)+ RNA purified on the oligo(dT) column was isolated (in two biological replicates) using Trizol reagent (Invitrogen). Purification of poly(A)+ RNA on the oligo(dT) column, cDNA library preparation with random primers and single-end deep sequencing on Illumina Novaseq 6000 were carried out by GENEWIZ (https://www.genewiz.com) resulting in the 77.5-88.5 million 150 nt paired-end reads.