GSM6506794: Hypoxia; Mus musculus; RNA-Seq
Explanted NG2tdT lungs were finely diced and digested for 30min in a mix of collagenase A/DNAse I. A single cell suspension was generated by filtering lungs through 70 micron and 40 micron filters and resuspending cells in PBS containing 0.04% BSA. Cells were loaded into the 10X Genomics Chromium instrument per manufacturer's directions. Libraries were constructed according to Chromium Next GEM Single Cell 3' Kit v 3.1 and performed by Stanford Functional Genomics Facility.