Decomposing Sargassum
Ultima Genomics (UG) compatible, PCR-free libraries were generated starting with approximately 500 ng of genomic DNA. The DNA was enzymatically fragmented, end-repaired, and a-tailed using the NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs).  xGEN PCR-free adapters for Ultima Genomics (Integrated DNA Technologies) were ligated, and resulting library molecules were cleaned up using a double-sided SPRI with average insert sizes of approximately 350-400 bp. Library size was determined by running 1 L on a High Sensitivity D1000 TapeStation (Agilent Technologies), and the library was quantified using the NEBNext Library Quantification Kit (New England Biolabs).