GSM6622784: P16_DP_Rep1; Mus musculus; RNA-Seq
Total RNA was purified from sorted cells with the Absolutely RNA Nanoprep Kit (Agilent). RNA concentrations were measured with a NanoDrop spectrophotometer (Thermo), and quality was assessed with an Agilent Bio-analyzer. Samples with RNA integrity number score >8 were further processed. 6 ng of starting material was reverse transcribed and amplified with the RNA Ovation RNAseq System V2 (NuGEN). From 100 ng of sheared amplified cDNA, sequencing libraries were generated with 6 unique barcoded adaptors, one for each sample (2 biological replicates x 3 populations) using the Ovation Ultralow DR Library System (NuGEN).