Nicotiana tabacum + Physochaina orientalis hybrids
Total DNA extracted from calli and leaves of a selected somatic hybrid line named ntpoA01 was subjected to high-throughput sequencing using the Illumina HiSeq 2500 platform at the Beijing Genomics Institute. A total of 6.6 Gb data containing ~26.5M clean 2x125 bp paired-end (PE) reads with an average insert size of ~800 bp were generated.