16S of Lake Huron mussel feeding experiments
we sequenced the V4 region of the 16S rRNA gene using the 515F/806R primer set (developed by Kozich et al. 2013) at the Microbiome Core at the University of Michigan Medical School, Ann Arbor, MI using the MiSeq Reagent Kit v2 (500-cycles) (Cat. number: MS-102-2003).