WGS human gut metagenome visit4
DNA was extracted from human stool using chemagic DNA Stool Kit (PerkinElmer chemagen, Baesweiler, Germany) combined with a previous bashind bead step. The Illumina DNA PCR-Free Library Prep Kit was utilized for library preparation. Library selection was based on Illumina tagmentation process. Average fragment size of 450 bp was used for every sample. Before proceeding to the next step, the pool was denatured and diluted according to the manufacturer's instructions. Finally, the library and PhiX control were combined in a single reaction vessel with a PhiX fraction of 1%. Sequencing was performed on the NovaSeq 6000 platform using 300 cycles of S1 chemistry.