DNA-Seq of human subgingival microbiome: pre-eclampsia
Subgingival plaque samples were collected and pooled from 15 sites on six maxillary and mandibular anterior teeth using sterile endodontic paper-points. DNA was isolated using the MagMAX Total Nucleic Acid Isolation Kit and quantified using a Qubit 3.0 fluorometer. Quantified and pooled libraries were clustered on the Illumina HiSeq 4000 for 150bp PE sequencing.