GSM7390091: Exosomes, SCLC 5; Homo sapiens; miRNA-Seq
Isolation of exosomes from serum samples were centrifuged at 10,000 × g for 30 min at 4 °C to completely eliminate cellular debris. Thereafter, the supernatant was centrifuged at 100,000 × g for 70 min at 4 °C. The pellets were washed with phosphate-buffered saline (PBS), ultracentrifuged, and re-suspended in PBS. Exosomal RNA was harvested using Trizol reagent (Invitrogen). Total RNA was used for the construction of sequencing libraries. RNA libraries were prepared for sequencing using NEBNext Multiplex Small  RNA Library Prep kit (New England BioLabs, Inc., USA) according to the manufacturer' instructions.