GSM8084448: DN003, SE_LAG3_KO_TumorCells; Homo sapiens; RNA-Seq
Total RNA was extracted with RNeasy mini kit (QIAGEN) according to manufacturer's instructions. RNA library preparations and sequencing reactions were conducted at GENEWIZ, LLC. (South Plainfield, NJ, USA). SMART-Seq v4 Ultra Low Input Kit for Sequencing was used for full-length cDNA synthesis and amplification (Clontech, Mountain View, CA) and Illumina Nextera XT library was used for sequencing library preparation.