GSM7804883: Flow, scRNA-seq; Homo sapiens; RNA-Seq
Micro-small intestine systems were trypsinized to dissociate into single cells. These cells were mixed and the cell concentration was adjusted to 1,000 cells/μL and applied to a Chromium 10X Single Cell System (10X Genomics) to generate the Gel-Bead in Emulsions (GEMs). cDNA libraries were prepared using a Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1 (10X Genomics) following the manufacturer's instructions.