GSM7180321: Human intestinal organoid, Wnt2b homozygous nonsense mutation, biol rep 3; Homo sapiens; RNA-Seq
Bulk RNA was extracted using an RNeasy Plus Micro Kit (Qiagen) according to manufacturer guidelines. 200 ng of RNA prepared into libraries following Illumina Stranded mRNA Prep protocol with all recommended reagents. Final library amplification step using 5 uL index anchors and 13 PCR cycles. Individual libraries were quantified using Qubit® dsDNA HS assay and run on the Agilent Fragment Analyzer for library bp size. Libraries were pooled using the percent range adjusted concentration of fragments 175-650 bp. RNA-seq. Sequencing was run on Nova-SeqTM 6000 PE-100.