GSM6896319: Nasopharyngeal tissue, negative, sample 3; Homo sapiens; RNA-Seq
140 µL of a nasopharyngeal swab in a viral transport medium (VTM) is extracted with QIAamp Viral RNA Mini Kit (QIAGEN, Germany) using the factory protocol. An 60 µL elution of total RNA was acquired. The RNA integrity and concentration were then measured using the Agilent RNA 6000 Pico Kit on The Agilent 2100 Bioanalyzer system (Agilent Technologies, Inc., USA) and the Qubit dsDNA HS (High Sensitivity) assay on the Qubit 4 Fluorometer system (ThermoFisher Scientific, USA) respectively. 11 uL of 10-100 ng of RNA was used as the cDNA library preparation and pooling input. The pooled library was prepared using the Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus (Illumina, USA).