GSM7016866: Monocyte_RNA_Plas_CLP rep1; Mus musculus; RNA-Seq
Different cells were sorted by BD Aria III with the following surface markers. HSPCs were sorted from Lin- cKit+ CD34+ population. GMPs were sorted from Lin-Ckit+Sca1-Cd16/32+CD34- population. Monocytes were sorted from CD45+CD11b+Ly6Chi Ly6G- population. Library preparation was performed using the Illumina TruSeq stranded mRNA kit with a starting input of 300ng and libraries were sequenced on the IlluminaNovaSeq 6000 instrument generating an average of 20 million reads per sample.