Metabarcoding of fungal communities: wheat seed
Fungal metabarcoding of wheat seeds was accomplished using ITS1 primers (Forward: ITS-1F_KYO2: AGAGGAAGTAAAAGTCGTAA, Reverse: ITS86R: TTCAAAGATTCGATGATTCAC). Samples were sequenced on four separate Miseq v2 2 x 250 bp paired-end format sequencing runs at a similar read depth.