GSM7659547: CEL211011EL_AML_3_ADT; Mus musculus; RNA-Seq
Spleens from mice were collected from healthy WT or AML mice and processed for single cell suspensions. In brief, spleens were mechanicaly disrupted using frosted tip glass slides and filtered using 70 um mesh filters. Filtered suspensions were ACK treated to remove RBCs before incubation with ADTs according to manufacturer's instructions (BioLegend). After ADT surface stains cell suspensions were resuspended at 1,000 cells per µL with 1X PBS 0.04% BSA before transfer to OHSU MPSSR for sequencing. scRNA-seq 3'GEX v3 (10x Genomics) Library was performed according to the manufacter's instructions (Single Cell 3'GEX v3 protocol, 10x Genomics).