RNAseq_DEB135-10_ITGA6_neg_r2
Following the 7 day differentiation, Defined Keratinocyte SFM Media is given up to Day 45 at which time the cells are enriched with ITGA6 antibody for the keratinocytes. iSC population is the positive fraction while the reduced population is the negative fraction. Both Pos and Neg Fractions were expanded in Defined Keratinocyte SFM.  iPSCs were expanded in StemFit Basic 02 media before differentiated in Essential 6 media treated with BMP-4 and Retinoic Acid for 7 days to differentiate the cells toward keratinocytes. RNA was isolated using Trizol per manufacturer protocol. The aqueous phase was transferred  and wahsed using an RNAeasy spin colunm. DNA was removed with a Qiagen Dnase kit on the Rneasy column. Libraries were consructed with the KAPA kit for PolyA enriched mRNA per manufacterer instructions.