GSM7017317: Sox10Cre_BtR_IOM_uninjured_Red, scRNAseq; Danio rerio; RNA-Seq
Microdissected joints were mechanically and enzymatically dissociated prior to FACS sorting with either enrichment for DsRed+ cranial neural crest (CNCC) lineage cells or no enrichment in BFP+ libraries. FACS-sorted cells were processed using the 10X Chromium controller for single cell profiling. Barcoded single cell cDNA library constuction performed as per manufacturer's instructions using the 10X scRNAseq Kit v2.