metagenome WGS on different substrate:gp time 5
The library was generated from 100 ng of genomic DNA,in accordance with the NEBNext Ultra II DNA LibraryPrep Kit for Illumina protocol (New England Biolabs). Prior to library preparation, DNA was fragmented using NEBNext dsDNA Fragmentase for 30 min. NEBNext Multiplex Oligos for Illumina (Dual Index Primers set 1)were used to produce the library and label it with specific molecular barcodes. Then, AMPure XP beads (BeckmanCoulter) were used for library purification. Finally, the library was quantified using the High Sensitivity DNA Kit(Agilent Technologies) on the Bioanalyzer instrument (Agilent). Sequencing was performed with an IlluminaNextSeq 500 sequencer with 2  150-bp read, using NextSeq 500/550 Mid Output Kit v2