GSM8306061: NPC cells,Control,E15.5,biol rep 2; Mus musculus; RNA-Seq
E15.5 embryonic kidneys were dissected and cut into small pieces, trypsinized for 30 min at 37℃, and trypsinization was terminated by adding DMEM/F12 supplemented with 10% FBS. After centrifugation, cells were resuspended in DMEM/F12 supplemented with 2% FBS and filtered 2 times through 70µm cell-strainer cap (Biosharp, BS-70-XBS). The single cell suspension was transferred into a polypropylene tube (Falcon, 352063) and kept on ice until FACS. The GFP+ cells were isolated into DMEM/F12 supplemented with 10% FBS using Sony MA900 Cell Sorter. The RNeasy Micro Kit (catalog no. 74004, Qiagen, Hilden, Germany) was used to isolate RNA. Library construction and RNA sequencing were made by OE Biotech (Shanghai, China).