NextSeq 500 sequencing; Single-cell RNA sequencing of mouse embryonic cells from the oocyte, 2-cell, 4-cell, 8-cell, blastocyst, and morula stages
Single-cell RNA sequencing of mouse embryonic cells from the oocyte, 2-cell, 4-cell, 8-cell, blastocyst, and morula stages
After IVF has been performed and embryos are cultured until certain stage of development before placing them In lysis buffer. Nucleic acid extraction protocol has been done according to the new STRT-N RNA seq method (manuscript in submission). Nucleic acid library construction protocol has been done according to the new STRT-N RNA seq method (manuscript in submission).