Euglossine RADseq 452
RADseq was performed using a protocol modified from Poland et al. 2012. After DNA extraction, restriction digest using the enzymes PstI & MspI was performed. This was followed by adaptor ligation, cleaning, PCR to add unique barcodes, cleaning, pooling, size selection, cleaning, and sequencing on an Illumina NovaSeq. SNPs were identified deNovo, using the STACKS pipeline.