16S Amplicon of tidal marsh sediment
Soil samples were homogenized with 100 mL of 1X PBS. DNA was extracted from all samples using the Qiagen DNeasy Power Soil Pro kit. The extracted DNA was sent to UCONN Core Sequencing facility for amplicon sequencing of the 16S rRNA gene in the V3-V4 region. The forward sequence reads were processed using a MOTHUR pipeline (Schloss et al., 2009). Forward sequences were trimmed to 130-200 bp range, ambiguous nucleotides were removed, and then OTUs with a 3% dissimilarity were created.