Bos taurus blood metagenome
Total nucleic acids from 200 ul serum isolated with HighPure Viral Nucleic Acid Isolation kit (Roche); 930 ng total eluate subjected to RCA (rolling circle amplification) with phi29 polymerase and random hexamer primers; 315 ng double stranded DNA product used to prepare Illumina fragment library (Illumina DNA Prep M Tagmentation Kit with Nextera DNA CD indices); Sequencing on a NextSeq550 sequencer with a NextSeq Mid Output Flowcell (300 cycles);