GSM6510130: KO Biorep3; Plasmodium falciparum; RNA-Seq
On day 15 of gametocyte development, stage V gametocytes were harvested using saponin lysis. Total RNA from saponin-lysed parasites was extracted using Trizol (Invitrogen) and QIAGEN RNA-extraction kit. Following RNA isolation, total RNA integrity was checked using a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). RNA concentrations were measured using the NanoDrop system (Thermo Fisher Scientific, Inc., Waltham, MA). Libraries were constructed using Illumina Ribo Zero Gold for human/mouse/rat kit. The libraries were multiplexed and clustered on one lane of a flowcell and loaded on an Illumina HiSeq platform paired-end 150bp reads. Sequencing of libraries was conducted by GENEWIZ (South Plainfield, NJ).