GSM8305806: Scr PC, no treatment 1; Homo sapiens; RNA-Seq
Total RNA was isolated using PureLink RNA Kit (Invitrogen). Samples with RIN values greater than 9 were used for library preparation. mRNA library was prepared from ~200 ng RNA using KAPA mRNA HyperPrep kit (Roche). Indexed libraries were quantified by KAPA Library Quantification kit (Roche), and insert size distribution was determined with the Agilent 2100 Bioanalyzer. Samples with a yield of  ≥ 0.5 ng/µl and a size distribution of 150-300 bp were used for sequencing using NovaSeq6000 sequencer (Illumina).