GSM8387221: Dhx16_fl3; Mus musculus; OTHER
Total RNA was isolated using TRIzol reagent (Takara). The full length transcripts were prepared using a custom protocol based on Smart-seq2. VN-anchored oligo-dT primers and TSO were used for reverse transcription (RT) of total RNA. The resulting full-length double-stranded cDNA was pre-amplified using ISPCR primers and purified with AMPure XP beads to remove primer dimers.The full-length cDNA was end-repaired, and SMRTbell adapters were ligated.Unligated fragments were digested with exonuclease, and the library was purified again using magnetic beads to obtain the sequencing library.After the library construction was completed, the quality of the library was checked. Sequencing was performed only after the quality was confirmed to meet the requirements.