GSM7870819: Integrated sgRNAs in ANKL1 cells before establishment of ANKL-PDX #3; Homo sapiens; OTHER
Liver was obtained from ANKL1-PDX mouse, followed by degradaded with 1 mg/mL of collagenase and 20 U/mL of DNase I. After density gradient centrifugation with 35% Percoll to remove hepatocytes, ANKL1 cells were harvested using autoMACS after stained with a biotin conjugated anti human CD45 antibody and anti-biotin microbeads. Genomic DNA was extracted using DNeasy blood and tissue kit. After amplification of integrated sgRNAs using TaKaRa Gflex DNA polymerase, illumina i7 index primers were added.