GSM8268692: WT_12dpa; Mus musculus; RNA-Seq
Digit tip blastemas were manually dissected followed by trypsin and collagenase I enzymatic digestion. Red blood cells were lysed with ACK lysis buffer. Dissociated single cells were washed, filtered and resuspended in 0.4% BSA in PBS then FACS sorted with 1ug/mL propidium iodide. Live cells were collected and adjusted to concentration of 1000 cells/uL for single cell RNAseq library construction. All samples used the 10x Chromium transcriptomics platform (10x Genomics) implemented by the Brigham and Women's Hospital Single Cell Genomics Core. 10x Chromium Single Cell 3' v3 chemistry was used per manufacturer's protocol.