ChIP-seq of E. coli: BrxX precipitation
Bacteria expressing Strep-tagged BrxX methyltransferase were infected with phage T7 (0.3-0.7 genes fusion strain). After formaldehyde treatment, Strep-tagged BrxX was purified by affinity chromatography and the precipitated DNA was sequenced. DNA libraries were prepared according to a standard protocol and sequenced on a MiniSeq platform (Illumina, USA) with a paired-end sequence of 150 cycles (75 + 75).