GSM7871944: Resistant_GNE-7883_rep2; Homo sapiens; RNA-Seq
Cell lines were lysed on-plate for RNA isolation using the Qiagen RNeasy Plus Mini kit (Cat. No. 74034), and RNA concentration was determined using a NanoDrop 8000 (Thermo Fisher Scientific).  RNA integrity was confirmed using a “Bioanalyzer 2100 (Agilent Technologies). About 500 ng of RNA was used for library synthesis using the TrueSeq RNA Sample Preparation kit v2 (Illumina). Size of the libraries was confirmed using 2200 TapeStation and High Sensitivity D1K screen tape (Agilent Technologies), and the concentration was determined by a qPCR-based method using the Library Quantification Kit (KAPA). The libraries were multiplexed and then sequenced on Illumina HiSeq2500 (Illumina) to generate an average of 56 million single-end 50-base reads per sample.