RNA-Seq if FE-1H10 neg CD8 T cells-N1
RNA was extracted from FE-1H10- CD8+ T cells by illustraRNAspin Mini RNA Isolation Kit. The poly(A) mRNA isolation was performed using Poly(A) mRNA Magnetic Isolation Module or rRNA removal Kit. First strand cDNA was synthesized using ProtoScript II Reverse Transcriptase and the second-strand cDNA was synthesized using Second Strand Synthesis Enzyme Mix.