GSM7804556: immune cells, EL4 subcutaneous tumour, psgl1 ko, rep6; Mus musculus; RNA-Seq
almost 20 days after inoculation, graft EL4 tumour tissues were resected and digested into single cell suspension. After that, CD45 positive immune cells were sorted for RNA-seq with flow cytometry. Total RNA was extracted from sorted immune cells with TRIzol reagent (Invitrogen) according to the manufacturer's instructions, and >1 ug of total RNA was used for the construction of sequencing libraries. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® following manufacturer's recommendations and index codes were added to attribute sequences to each sample.