GSM8388756: Illumina-SNF2h-KD-IAA_R1; Toxoplasma gondii; RNA-Seq
Total RNAs were extracted and purified using TRIzol (Invitrogen, Carlsbad, CA, USA) and RNeasy Plus Mini Kit (Qiagen). Nanopore: the mRNA library preparation followed the SQK-RNA004 kit (Oxford Nanopore)–recommended protocol, the only modification was the input mRNA quantity increased from 500 to 900 ng (300ng from each replicate), and all other consumables and parameters were standard. | Illumina: RNA sequencing was performed following standard Illumina protocols, by Novogene (Cambridge, United Kingdom).