GSM7978009: Palatal shelves from GD12 embryos, V3; Mus musculus; RNA-Seq
Palatal shelves from GD12 embryos were microdissected under a stereomicroscope, snap-frozen in liquid nitrogen, and then stored at −80 °C. Embryos were genotyped, and palatal shelves from two embryos of the same genotype were pooled for RNA extraction. Three RNA samples (from six embryos) for each group were used for subsequent sequencing. No more than three embryos of the same genotype were used from a single litter, and embryos from at least three litters were included for each genotype. RNA was isolated using the Illustra RNAspin kit (GE) using the manufacturer recommendations with on-column DNase I digestion. RNA was eluted in 30 μL of water, and RNA concentration was quantified using a Qubit fluorometer (ThermoFisher Scientific). Approximately 100 ng of total RNA  from three pooled RNA samples (from six embryos) for each group was used for sequence library construction following instructions of the NuGen mRNA sample prep kit (cat# 0348).