GSM6621065: M28Z-BKO_multiround_2; Homo sapiens; RNA-Seq
Total RNA was isolated from CAR-T cells and mRNA was enriched with Oligo (dT) and broken randomly, then mRNA was reversed by random hexamers to cDNA. Terminal of the double-stranded cDNA was added sequencing adaptors and utilized PCR amplification to produce the cDNA library.