GSM7802488: G2_miRNA-seq; Pinellia ternata; miRNA-Seq
Trizol reagent (Sangon Biotech, Shanghai, China) was used for the extraction of total RNA from tissue samples. Six samples (C and G, each with three biological replicates) were harvested for small RNA library construction and sequencing. Equal amounts of RNA from each sample were sequenced on an Illumina HiSeq2500 sequencer (Illumina, San Diego, CA, USA) at the Sangon Biotech company (Shanghai China), to construct the strand-specific RNA-seq. 1.0 μg of total RNA was used to prepare a small RNA library according to the instruction of TruSeq Small RNA Sample Prep Kits.