GSM7804678: QR27, case; Homo sapiens; miRNA-Seq
Total RNA extraction with miRNA enrichment was performed using the Vana™ miRNA Isolation Kit (Thermo Fisher Scientific, AM1561). Approximately 1 ug of total RNA was used to prepare small RNA library according to the protocol of TruSeq Small RNA Sample Prep Kits (Illumina, San Diego, CA, USA) and single-end sequencing 50bp on an Illumina Hiseq 2500 (following the vendor's recommended protocol) was conducted