GSM6592093: PGE2TL_C; Homo sapiens; RNA-Seq
RNA from human cDC1 was isolated using the NEB Monarch Total RNA Miniprep Kit. Isolated mRNA from human cDC1 was fragmented and the first strand of cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dTTP for non-directional library preparation. Following end repair, A-tailing, adapter ligation, size selection, amplification, and purification, the library was checked with Qubit and real-time PCR for quantification, as well as bioanalyzer for size distribution detection. Quantified libraries were pooled and sequenced on Illumina platforms, according to effective library concentration and data amount.