scRNA-Seq of homo sapien: adult male primary airway bronchial-tracheal epithelial cells
Libraries were constructed following 10x manufacturing instructions. Involved cell counting, Chip G gel bead-in-emulsion, cDNA synthesis (with UMI, 10X barcode and Illumina R1 primer sequence), cleanup, and library preparation (Illumina TruSeq adaptor, PCR). Sequenced on NovaSeq for 30000 read pairs per cell.