short reads Illumina sequencing
A PCR-free library was prepared using the Kapa Hyper Prep Kit (Roche, Basel, Switzerland). Genomic DNA (1.2g) was sonicated using a Covaris E220 sonicator (Covaris, Woburn, MA, USA). Fragments were end-repaired and 3-adenylated, and Illumina adapters (Bioo Scientific, Austin, TX, USA) were ligated according to the manufacturers instructions. Ligation products were purified twice with AMPure XP beads (Beckman Coulter Genomics, Danvers, MA, USA) and quantified by qPCR (MxPro, Agilent Technologies, Santa Clara, CA, USA) using the KAPA Library Quantification Kit for Illumina Libraries (Roche).