GSM6896353: Monocytes_control_T407; Homo sapiens; RNA-Seq
Circulating monocytes were isolated from total blood by FACS. Total RNA were purified according the miRNeasy Micro Kit (P/N 217084, Qiagen Courtaboeuf, France) including a RNAse free DNase treatment from. RNA sequencing libraries were prepared from 100 to 200 ng of total RNA using the Illumina® Stranded Total RNA Prep, Ligation with Ribo-Zero Plus library preparation kit. Sequencing was carried out using a pair-end 2 x 100 bp mode on the NovaSeq 6000 system (Illumina), targeting between 10 and 15M clusters per sample, strandness reverse