Illumina NovaSeq 6000 sequencing; Gene expression of early memory and effector precursor CD8 T cells generated by differential TCR signaling strength
Gene expression of early memory and effector precursor CD8 T cells generated by differential TCR signaling strength
CD62L+TCF1+ and CD62L-TCF1- cells derived from either antibody-induced (AB) activation or from gp33 (high affinity) or C6 (low affinity) peptide stimulation were sorted on day 6 post activation. Qiagen Micro RNA kit SmartSeq2 kit was used for library preparation