GSM8389183: IL-33 cKO MOG-GP_day21_rep1; Mus musculus; RNA-Seq
To perform RNA-seq, we FACS-sorted brain CD8 GP33-41+ T cells from WT, MOG-GP, and IL-33 cKO MOG-GP mice at 21 days after rLCMV-GP33 infection. Each biological replicate (n = 3 for MOG-GP, n = 3 for IL-33 cKO MOG-GP and n = 1 pool of 4 WT mice) represents 104 sorted H-2Db-GP33-41-specific CD8+ T cells in RLT buffer with 1% ß-mercaptoethanol and processed with RNAeasy Micro Kit (Qiagen, 74004) following manufacturer's instructions. RNA libraries were prepared for sequencing using standard Illumina protocols