Hi-C of HeLa-S3
The Hi-C library was generated using Phase Genomics Proximo Human Kit version 4.0. Cells were cross-linked for 15 minutes at room temperature, followed by quenching with a quenching solution for 20 minutes. The nuclear fraction was isolated, washed with 1X Chromatin Rinse Buffer (CRB), and then resuspended in Proximo Lysis Buffer 2 before incubation at 65C for 15 minutes. Chromatin was bound to Recovery Beads, fragmented, and ligated using Proximo fragmentation mix and Proximity ligation enzyme, respectively. After reversing crosslinks, DNA was purified and Hi-C junctions were bound to streptavidin beads for the preparation of paired-end deep sequencing libraries using Proximo Library preparation reagents.