C1
Agilent MC-seq was applied to assess the methylation status in the nine cell samples (Santa Clara, CA, USA). Briefly, the DNA samples library was constructed. The concentration and size of the constructed library were measured with the Qubit 2.0 fluorometer and Agilent 2100 Bioanalyzer, respectively. Following bisulfite treatment and amplification by polymerase chain reaction (PCR), the DNA samples were then sequenced on Illumina NovaSeq6000 (Illumina, San Diego, CA, USA).The quality of sequence was assessed by Fastp v0.20.0 Software (https://github.com/OpenGene/fastp). Finally, the eligible differential methylation sites (DMSs) were identified and annotated with the use of the Dispersion Shrinkage and the ClusterProfiler package.