RNA seq of GG with and without POM
Cells were transferred twice in SDM 1% G and then cells were grown for 8 hours in SDM with 0.5% G at 37C under ambient atmospheric condition. Cells were pelleted and flash frozen. RNA was extracted with Zymo Direct-zol RNA miniprep kit, treated with Turbo DNase I and column-purified. RNA purity was confirmed via Bioanalyzer and sent to UIUC for sequencing.