GSM8269479: control diet, liver endothelial cells, biol rep 1; Mus musculus; RNA-Seq
Mice were anesthetized with ketamine/xylazine and decapitated. One lobe of the liver was dissected.  A series of enzymatic and mechanical steps was used to achieve a single cell suspension of the liver tissue. FACS was used to enrich for CD31+ endothelial cells, which were sorted into trizol for RNA extraction. RNA was extracted using the RNeasy Micro kit from Qiagen Non-stranded mRNA libraries were made using Illumina's TruSeq RNA Library Prep Kit v2 according to manufacturer's instructions.