16S amplicon of Vespula germanica's gut
16S amplicon sequencing using a MetaVX Library Preparation Kit was conducted on an Illumina MiSeq Platform (Illumina, San Diego, USA) by Azenta Life Sciences (Suzhou, China). Amplicons cover the V3 and V4 hypervariable regions of the 16s rRNA gene of bacteria. Automated cluster generation and 250 paired-end sequencing with dual reads were carried out following the manufacturers instructions. Double-end sequencing was then performed on both positive and negative reads, with the initial read joining. Sequences containing ambiguous bases (sequence of N) were filtered out, retaining only sequences > 200 base pairs.