Individual B: DNA sequencing from B19, library L003
DNA was extracted using MagMAX DNA Multi-Sample Ultra kit (Applied Biosystems). Libraries were prepared from 10 ng single colony-derived DNA using the Qiagen DNA FX library preparation kit with unique dual indexes (UDI).  Fragmentation was run for 16 minutes with no enhancer. Adapters were diluted 1:10 and samples were PCR amplified for 10 cycles. Paired-end whole genome sequencing was performed (150 bp) at 15x targeted depth of coverage on the Illumina NovaSeq v1.5 kits (S4, 300 cycle).