Pestalotiopsis trachicarpicola treated with cymoxanil for 12 hours, with a final concentration of EC90
Mycelial plugs (4 mm diameter) from the edge of 3-day-old colony of the strain ACCC 35058 was transferred to PDB medium,with a final concentration of EC90, and inoculated in a incubator at 25C for 5 d. Mycelia were ground into fine powder under the protection of liquid nitrogen. About 100 mg mycelia powder were used for RNA extraction. Total RNA was extracted using Trizol reagent (thermofisher, 15596018) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA, 5067-1511) , high-quality RNA samples with RIN number > 7.0 were used to construct sequencing library.