Whole Genome Sequencing  of iPSCs from subject S2
Genomic DNA was extracted from each of the 6 lines and processed separately. Genomic DNA samples were quantified using the Qubit Flourometer 3.0 and approximately 25ng of genomic DNA was used for sample assessment. Genomic DNA (~600ng) was fragmented on the Covaris LE220 instrument targeting ~375bp inserts. A QC check was done post fragmentation to assess DNA quality using the Agilent Bioanaylyzer. Automated dual indexed libraries were constructed with the KAPA Hyper PCR-free library prep kit (ROCHE DIAGNOSTICS SYSTEMS, Cat # 7962371001) on the SciCloneG3 NGS instrument platform (Perkin Elmer) in a 96 well format. The concentration of each library was accurately determined through qPCR utilizing the KAPA library Quantification Kit according to the manufacturer's protocol (KAPA Biosystems/Roche) to produce cluster counts appropriate for the Illumina NovaSeq6000 instrument. Normalized libraries were sequenced on a NovaSeq6000 S4 Flow Cell using the XP workflow and a 151x10x10x151 sequencing recipe according to manufacturer protocol.