16S rRNA of fertilizers: made from animal feces or plant materials
Total bacterial genomic DNA in fertilizer sample was extracted as described. The V4 region of 16S rRNA genes was amplified and sequenced using the Illumina MiSeq platform (Illumina, San Diego, CA, USA) in Annoroad Gene Technology Co., Ltd (Beijing, China). The resulting 16S rRNA sequences were mainly analyzed based on QiiME2 workflows.