CHiCAGO data sets for Biscutella laevigata subsp. austriaca
Chicago libraries were generated following (Putnam et al., 2016), from ~500ng of high molecular weight DNA fixed with formaldehyde and digested with DpnII. The 5 overhangs were filled in with biotinylated nucleotides, whereas free blunt ends were ligated, and crosslinks were then reversed before the DNA was purified and treated to remove biotin that was not internal to ligated fragments. Sequencing libraries were then generated from sheared DNA at ~350 bp mean fragment size using NEBNext Ultra enzymes and Illumina-compatible adapters. Biotin-containing fragments were isolated using streptavidin beads before PCR enrichment of each library.