PromethION sequencing
HG002 (Coriell Institute, GM24385) was cultured in RPMI1640 (Gibco, 11875093) with 15% fetal bovine serum (Bovogen, SFBS-AU) at 37ï¿½C with 5% CO2. DNA was extracted using Nanobind CBB kit (PacBio, 102-301-900). DNA was sheared to ~33 kb using the Megaruptor 3 (Diagenode) and libraries prepared using SQK-LSK114 and sequenced on a R10.4.1 flow cell (Oxford Nanopore Technologies) at 5kHz sampling rate to generate ~40X genome coverage. This is the raw signal data in BLOW5 format.