Metagenomic of Caviahue Lake: sediments collected at the deepest depth of the lake at 0-2 cm
Amplification of the 16S rDNA V4 region was performed with the primers 515F and 806R in a reaction mixture (final volume 50 L) consisting of 100 ng of DNA, 10 M of each primer, 10 mM of dNTPs and 0.5 L of Herculase II Fusion DNA Polymerase (Agilent).