Groundwater amoeba 18S amplicon
PCR amplification of 18S gene using forward and reverse primers 5CAGCAGCCGCGGTAATTCC3 and 5CCCGTGTTGAGTCAAATTAAGC3,  targeting a 650 bp segment of the V4V5 region. Sequenced on a GridION with the SQK-NBD114.24 kit and an R10.4.1 Flow cell.