scRNA-seq of sus scrofa: female dorsal skin
Library selection is DNBelab C4. Approximately 20,000 cells were captured in droplet emulsions, and the mRNA of single-cell Libraries were constructed according to the DNBelab C Series Single-Cell Library Prep Set (MGI, Shenzhen, China). Briefly, single-cell suspensions were subjected to a series of progress, including droplet encapsulation, emulsion breakage, mRNA captured bead collection, reverse transcription, and cDNA amplification and purification, to generate barcoded libraries. Indexed sequencing libraries were established based on the instructions protocol.