SLAF Tetrastigma hemsleyanum
Total genomic DNA was extracted from the silica-dried leaf tissue using DNA Plantzol (Invitrogen) following the manufacturers protocol. Specific length amplified fragment (SLAF) sequencing libraries were prepared using two restriction enzymes RsaI and HaeIII. Specific single nucleotide A overhangs were added to the 3 end of the resulting fragments and connected to dual-index sequencing adaptors. The resulting digested fragments ranging from 314 to 414 base pairs (with barcodes and dual-index sequencing adaptors) in size were excised and purified using a Gel Extraction Kit (Qiagen). After the library quality inspection, paired-end sequencing with read lengths of 126 bp was performed using an Illumina HiSeq 2500 system (Illumina, Inc., San Diego, CA, United States) according to the manufacturers recommendations at Beijing Biomarker Technologies Corporation.