CS-319 R1 120HPI
The preparation of cDNA libraries from the samples was accomplished using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific, MA, USA) to generate RNA-seq data. Subsequently, double-stranded cDNA purification and size selection were performed using AM Pure XP beads (Invitrogen, CA, USA). To enrich the necessary size fragments, the library was amplified with barcoded primers. To create multiple fragment copies, 100 pM cDNA libraries were assigned to the emulsion PCR system performed on the Ion Torrent OT2 machine. The Ion Torrent S5 sequencer system (Thermo Fisher Scientific, MA, USA) was then utilized to sequence the generated fragments, employing the 540-chip