RNA-seq of C. elegans: adult day 7
Total RNA was treated with proteinase K (NEB) to increase purity. Libraries were made using the Illumina Stranded TruSeq RNA library prep kit. Prior to library preparations, ribosomal RNA was removed using RiboZero Gold (Illumina). cDNA libraries were sequenced on Illuminas NovaSeq 6000 (100 bp paired-end reads).