Bacterial 16S Metabarcoding Analysis of water from AWCC pond replicate 2
1 Liter of water from a pond at the Commonwealth of Virginias Aquatic Wildlife Conservation Center was passed through a 0.45 micron filter. DNA was extracted from the microbes caught on the filter using Qiagens Power Water kit. 16S prokaryotic barcodes were PCR amplified. Illumina sequencing, including library preparation, was completed by Azenta (South Plainfield, NJ, USA).