Pooled scRNA library batch B054-P1
Single-cell RNA-seq (GEX and HTO) libraries were generated using the 10x Genomics Chromium 3' Single Cell Gene Expression assay and Chromium Controller Instrument according to the manufacturer's published protocol with modifications for cell hashing (Stoeckius et al., 2018). Each batch contains 2 pools of samples (Pool 1: P1, Pool 2: P2) in which cells from upto n = 12 hashed samples with distinct Hashtag Antibodies were pooled together. Additionaly, a common batch control sample was added to each pool. Libraries were sequenced on an Illuminia Novaseq platform. Hashed 10x Genomics scRNA-seq data processing was carried out using CellRanger (10x Genomics) and BarWare36 to generate sample-specific output file.