ITS METAGENOMICS
The amplicons from each sample were purified withAmpure beads to removeunused primers and an additional 8 cycles of PCR was performed using Illuminabarcoded adapters to prepare the sequencing libraries. Libraries were purifiedusing Ampure beads and quantitated using Qubit dsDNA High Sensitivity assaykit. Sequencing was performed using Illumina Miseq  v3 v4sequencing kit.