Exon capture: Protium subserratum: Herbarium leaf material
Sequencing libraries were prepared using NEBNext FFPE DNA Repair Mix in combination with the NEBNext Ultra II DNA Library Prep kits for Illumina (New England Biolabs, Ipswich, MA, USA). Libraries were multiplexed for hybridization. Each pool was divided into two or three separate reactions for enrichment using a Custom MYBaits target capture kit following the manufacturers protocol with slight modifications. Each reaction was amplified for 16 cycles. Final libraries were pooled in equimolar quantities and sequenced on two lanes of Illumina Nextseq500 75bp SR at the Oklahoma Medical Research Foundation Clinical Genomics Center (Oklahoma City, OK, USA).