Nanopore sequencing of mock sample:Routine_PCA
Genomic DNA were extraction from HEK293T cells with DNeasy Blood & Tissue Kit (Qiagen, 69504); microbial genomic DNA of Staphylococcus epidermidis, Rosemonas mucosa, Staphylococcus hominis and Staphylococcus aureus were extraction with QIAamp DNA Microbiome Kit (Qiagen, 51704); genomic DNA of other microorganisms were extracted by Rapid Bacterial Genomic DNA Isolation Kit (Sangon Biotech, B518225-0050). All procedures were carried out according to the manufacturer instructions. The high molecular weight DNA were enriched through 0.5x VAHTS DNA Clean Beads (vazyme, N411). DNA concentrations were determined using the Qubit dsDNA HS (High Sensitivity) assay kit (ThermoFisher, Q32851); DNA purity was measured by NanoDrop based on the assessment curve shape and the ratio of OD 260/280 and OD 260/230. DNA fragment distribution was determined by Qsep100 (Bioptic) with S3 Cartridge (Kilo Base Cartridge, C105106).In this sample, it was sequenced by nanopore in routine setting and the data was used to analyze signals with Principal Component Analysis.