Gut Microbiome Signature in Indian Multiple Sclerosis
The fecal samples were obtained from the participants who were provided with a sterile empty container and were instructed to transport the samples to the laboratory under refrigerated conditions. The fecal samples were then coded for blinding, and frozen at -80C until further processing for their microbial DNA isolation by using QIAamp PowerFecal Pro DNA Kit (Qiagen, Cat. No. 51804) as per manufactures instructions. The DNA samples were then stored at -20C until 16S metagenomic sequencing.