soil metagenome
universal primer pairs were employed, i.e., 515F (5- GTGYCAGCMGCCGCGGTAA-3) and 907R (5- CCGYCAATTYMTTTRAGTTT-3) targeting the V4-V5 hypervariable region of 16S rRNA genes, ITS5F (5-GGAAGTAAAAGTCGTAACAAGG-3) and ITS1R (5-GCTGCGTTCTTCATCGATGC-3) targeting the internal transcribed spacer (ITS) sequence between 5.8S and 28S rRNA genes, and TAReuk454FWD1 (5-CCAGCASCYGCGGTAATTCC-3) and TAReukREV3 (5-ACTTTCGTTCTTGA TYRA-3) targeting the V4 region of 18S rRNA genes. A two-step polymerase chain reaction (PCR) amplification method was utilized to enhance the accuracy and quantification of the sequence data for library preparation.