WGS of Mycobacterium tuberculosis: isolate
The strain was cultured in Middlebrook 7H9 broth in order to perform DNA extraction using Zymo Genomic DNA Clean & Concentrator (D4010, D4011) kit; long reads sequencing was performed with MinION Mk1B platform (Oxford Nanopore Technologies, Oxford, UK) with a FLO-MIN106 R9.4.1 flow cell and using Rapid Barcoding Kit (SQK-RBK004) for library preparation