Amplicon Sequencing of SARS-CoV-2: Wastewater
Virus capture using Ceres Beads, extracted with RNEasy Mini Qiagen kit. cDNA synthesized with Lunascript RT supermix (NEB) and PCR amplified using Q5 2x Mastermix (NEB) and ARTIC V4 primers. Library prep using Illumina DNA prep kit.