Amplicon of sludge: Waste activated sludge after 55 days of anaerobic fermentation
Under controlled reaction conditions, the reactor reached a steady state after 55 days of operation, indicating a stable production of VFAs. At this point, samples were extracted from both the Control and 200 mg/kg TSS Flr concentration reactors. Following DNA extraction and library construction, metagenomic sequencing was conducted, including sequencing quality control and genome assembly, as well as gene prediction, taxonomy, and functional annotation.