scRNA-seq of Adult Pigs: Epicardial Adipose Tissue
Hearts were removed, placed in Krebs bicarbonate buffer (0-4 C) and weighed. Then EAT was collected from occluded and non-occluded regions of the left circumflex coronary artery. To isolate RNA from adipose tissue, ~ 0.1 grams of snap-frozen EAT was used. The samples were then subjected to Bulk RNAseq and single Nucleus RNAseq followed by bioinformatic analysis.