DNA-Seq of Syzygium zeylanicum: chloroplast genome
Total genomic DNA was extracted from the dried leaf tissue using a modified cetyltrimethylammonium bromide (CTAB) method [13]. The extracted DNA was further purified using a Monarch Genomic DNA Purification Kit (New England Biolabs, #T3010) following the manufacturers protocol. DNA quality and quantity were assessed using a NanoDrop spectrophotometer (Thermo Fisher Scientific) and Qubit fluorometer (Invitrogen), respectively. High-quality DNA (A260/280 ratio of 1.8-2.0) was used for subsequent library preparation. The sequencing library was prepared using the NEBNext Ultra II DNA Library Prep kit (New England Biolabs, #E7645). Paired-end sequencing (2  150 bp) was performed on a MiSeq sequencer (Illumina, San Diego, CA, USA). Raw reads were quality-checked using FastQC v0.11.9 [14] and trimmed using Trimmomatic v0.39 [15] to remove low-quality reads and adapter sequences.