SARS-CoV-19 WGS
Library preparation consisted of measuring concentration with the Qubit dsDNA High-Sensitivity Assay Kit, quality assessment using the Agilent High Sensitivity DNA Kit and 2100 Bioanalyzer instrument, and standardization to 8 nM. The libraries were again quantified, then diluted to a final concentration of 4 nM, and loaded onto the MiSeq system at 10 pM.