Amplicon sequencing of sediments: JHW group
The V3-V4 region of the 16S rRNA were targeted for amplification using specific primers 338F/806R with barcodes. Sequencing libraries were generated using NEB Next Ultra II FS DNA PCR- free LibraryPrep Kit ( New England Biolabs, USA, Catalog #: E7430L) following manufacturer'srecommendations and indexes were added .