Single-cell RNA sequencing of Epi-iOSKM mice
Single-cell RNA sequencing was performed on the dorsal epidermis of 7-9-week-old mice in the second telogen phase of hair growth cycle. Total epidermal cells were collected from control mouse and from mouse with OSKM (Oct-4, Sox2, Klf4, c-Myc) and mCherry induction in the epidermis (Epi-iOSKM), and the cells were sorted by mCherry expression. mCherry-negative cells were sorted from the dorsal epidermis of control mice (Sorted control). mCherry-negative and -positive cells were respectively sorted based on their mCherry expression from the dorsal epidermis of Epi-iOSKM mice (sorted Epi-iOSKM mCherry-negative, sorted Epi-iOSKM mCherry-positive).