RNAseq of Sus scrofa: brain total RNA sequencing
Total RNA was extracted from Sus scrofa brain tissue sample by RNeasy Mini Kit (QIAGEN). RNA-seq library was constructed and NGS library was sequenced by Illumina NovaSeq instrument using 150 bp paired-end sequencing chemistry at Australian Genome Research Facility.  Trimming of adapters from sequence reads and quality filtering were performed by 'fastp' (version 0.20.1). Trimmed and quality filtered paired-end reads were aligned to the Sus scrofa genome assembly Ensembl release 98 using 'STAR' (version 2.7) with default parameters.