Focused validation CRISPR activation screen in SK-N-DZ neuroblastoma cells: gRNA distribution following Erastin treatment (4 M), replicate 2
Focused CRISPR activation validation screen in SK-N-DZ neuroblastoma cells. Guide RNA distribution of the focused library in Erastin-treated cells (4 M); replicate 2. SK-N-DZ cells stably expressing dCas9-VP64 and the p65-HSF1 co-activator were transduced with a focused CRISPRa library within a modified CROP-seq lentivirus backbone. After puromycin selection and recovery, cells were collected, genomic DNA extracted and amplicon libraries constructed for Illumina sequencing.