RNA-Seq of Crocidura russula, KS18-0143, Tissue pool
Tissue samples were first homogenized in Qiazol using the TissueLyser II instrument, and subsequently, total RNA was extracted employing the Agencourt RNAdvance Tissue Kit, a process carried out on the KingFisher Flex Purification System. The quantity and quality of the total RNA were then evaluated. In order to decrease the presence of host-derived ribosomal RNA (rRNA) and enhance the virus-to-background ratio in the total RNA before sequencing, we subjected it to treatment with the "pan mammalia" riboPOOL ribosomal depletion kit. The resultant rRNA-depleted total RNA was employed for library preparation using the Collibri Stranded RNA Library Prep Kit. These libraries were quantified, combined, and sequenced on a NovaSeq 6000 system in a 1100 base pair sequencing mode.