Metagenome
V3, V4, and V5 hypervariable regions of prokaryotic 16S rDNA were selected for generating amplicons and following taxonomy analysis. .The v3 and v4 regions were amplified using forward primers containing the sequence CCTACGGRRBGCASCAGKVRVGAAT and reverse primers containing the sequence GGACTACNVGGGTWTCTAATCC. The v4 and v5 regions were amplified using forward primers containing the sequence GTGYCAGCMGCCGCGGTAA and reverse primers containing the sequence CTTGTGCGGKCCCCCGYCAATTC.DNA libraries were multiplexed and loaded on an Illumina MiSeq instrument.