16s sequence of rhesus macaque gut microbiome from rectal swab TP1A RIb13
Bacterial 16S rRNA genes were amplified using bacteria-specific PCR primers for the hypervariable V3-V4 region (Klindworth et al., 2013). The amplifications, barcoding, and sequencing experiments were performed following established protocols (Kozich et al., 2013; Caporaso et al., 2012). Sequencing was performed on the Illumina MiSeq platform at the Yerkes Genomics Core at Emory University, resulting in 2 x 275 bp paired-end reads.