LCWGS of Brachyistius frenatus: library BFR_SAL_12
We extracted genomic DNA from all samples using a chloroform-based protocol with isopropanol precipitation (Sambrook et al., 1989) and confirmed DNA quality on a 0.7% agarose gel. Sequencing libraries were prepared from genomic DNA using half reactions of the NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs) and dual index primers. Prepared libraries were quantified via Qubit, fragment analyzed, pooled, and then sequenced (150 bp paired end) on an Illumina NovaSeq 6000 (S4) at the Vincent J. Coates Genomics Sequencing Lab at the University of California, Berkeley.