WGS of Lepraria neglecta: whole thallus
A size selection step using MagBind Total Pure NGS (Omega Bio-tek, Inc., Norcross, GA, USA)  was then completed with a 0.4:1 bead solution to extract ratio, a series of 70% ethanol washes, and the sample was eluted in 55 L of nuclease-free water (McKenzie et al. 2020). Library preparation was completed using the Ligation Sequencing Kit LKS-109 according to the manufacturers protocol and samples were run on the MinION platform using R.9.4.1 flow cells for 52 hours (Oxford Nanopore Technologies, Oxford, United Kingdom). Basecalling was conducted using guppy v5.0.7 with the SUP model (Oxford Nanopore Technologies, Oxford, United Kingdom).