Genome sequencing of wAlbB-Uju Wolbachia variant
Oxford Nanopore Technology (ONT) sequencing library preparation was carried out with a Rapid Sequencing Kit, SQK-RAD0004, Oxford Nanopore Technologies (ONT) using the Ultra-long read protocol with some modifications. In short, the library was made from 4 µg gDNA conditioned with 0.02% Triton-X100. DNA was tagmented with 1.5 µl transposome mix and 1µl of Rapid Sequencing Adaptor was then attached to DNA ends. The library was loaded on a MinION Flow Cell (R9.4.1, FLO-MIN106) and sequenced for 48 hours on a GridION instrument controlled by the MinKNOW software, v22.03.4 (ONT). Base-calling and demultiplexing were performed within MinKNOW using Guppy v4.0.11.