Follow-up samples of AML patients: adult female peripheral blood
Selection of somatic mutations. Amplified them using PCR in triplicate with molecular-tagged primers. Combined it in a single tube. Sequencing. The FASTQ files generated after sequencing were demultiplexed to separate reads from triplicates and amplicons. Wild-type and mutated sequences were obtained for each of the gene positions. The final MRD value was defined by the mutation with the highest VAF.