16S rDNA human fecal sample ARCOL
DNA amplification and sequencing of the 16S rRNA was performed according to the preparation guide from Illumina (Illumina Technical Note 15044223). Briefly, the V3-V4 region of 16S ribosomal DNA (rDNA) was amplified by PCR with the primers suggested by Klindworth et al. 2013 (Supplementary Table 1). Illumina sequencing adapters and dual-index barcodes (Nextera XT indices) were added to the amplicon target via PCR amplification. 16S sequencing was performed on Illumina MiSeq platform (HCLM, Santiago, Chile) using a 2 x 300 bp paired-end reagent kit.