The Influence of Substrate Type and Incubation Environment on the Microbial Community Composition of Erie, PA Sour Dough Bread Starter Cultures
Samples were prepared by amplifying a segment of approximately 300 base pairs (bp) of the V4 region of the 16S rRNA gene was amplified. This was achieved using the primers 515F (GTGYCAGCMGCCGCGGTAA) and 806R (GGACTACNVGGGTWTCTAAT). Sequencing was then performed using manufacturer guidelines using a MiSeq.