Single cell a/b chain TCR-seq of cultured TIL: Sample TIL_12 week
10x Genomics Single Cell Chromium V(D)J Enrichment libraries were sequenced on one Novaseq 6000 SP run. The samples have sequencing yield of more than 23 million reads per samples. The sequencing run was setup as a 26 cycles + 90 cycles asymmetric run. Demultiplexing was done allowing 1 mismatch in the barcodes. The analysis was performed with the Cell Ranger v6.0.2 software using the default parameters.