RNAseq MACT cells
RNA isolated from the treated cells was checked for integrity by using Agilent TapeStation 4200. The RIN was >8.1 (9.10.5) for all samples. The RNA was sent to the Center for Quantitative Life Sciences at Oregon State University for high-throughput sequencing. Library construction was done utilizing a QuantSeq 3 mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen, NH, USA). Sequencing was performed on an Illumina HiSeq3000 platform at 60 samples/lane. Quality control was performed as previously described20. Differential gene expression analysis was performed using the DESeq221 package in RStudio (v1.4). Differentially expressed genes were denoted as genes with log2 fold change > |0.5| and a false discovery rate (FDR) < 0.05.