Barcode sequences for sample: Environment: 30C pH 3, flask 3, TimePoint T4, Biological Replicate R1, Sequenced with illumina
We used the YeaStar Genomic Kit Protocol I (Zymo Research, #D2002) to extract gDNA from $~$ 1 ml of pelleted yeast cultures. We used a two-step PCR protocol to generate the Illumina-ready dual-indexed amplicon library tagged with UMIs (unique molecular identifiers) and with dual barcodes.