16S amplicon seq of Rhodnius prolixus L4 instar: gut
templates were amplified and barcoded using fused primers in a single PCR reaction, 18S blocking primer was used. PCR products were cleaned prior the pooling using AMPure XP (Beckman Coulter) magnetic beads. The library was additionally purified using Pippin Prep (Sage science) and sequenced on Illumina MiSeq using v2 Nano chemistry (500 cycles; in-house MisSeq platform).