Nanopore sequence of Paenibacillus sp. RC80
RC80 was isolated from soils air dried and archived in 1998 in the Harvard Forest, a temperate, deciduous forest in Petersham, MA (42.54N, 272.18W). RC80 was isolated on ISP2 medium and grown on 10% TSB to prepare genomic DNA. For gDNA extraction, the isolate was grown on 10% TSB liquid media at 30C, shaking at 150 rpm until an OD of 0.5 was reached. Cells were pelleted using centrifugation at 4000 rpm for 15 minutes, and genomic DNA was extracted using CTAB. The genome was sequenced by SeqCenter (MinION) using Nanopore R9 flow cells (R9.4.1) with SQK-LSK109 sequencing kit.