Bacterial 16S Metabarcoding Analysis of water from AWCC pond incubated in a fish tank  for 96 hours replicate 3
20 Liters of water were removed from a pond at the Commonwealth of Virginias Aquatic Wildlife Conservation Center  and incubated in a 10 gallon fish tank for 96 hours. 1 Liter of water was passed through a 0.45 micron filter. DNA was extracted from the microbes caught on the filter using Qiagens Power Water kit. 16S prokaryotic barcodes were PCR amplified. Illumina sequencing, including library preparation, was completed by Azenta (South Plainfield, NJ, USA).