16S rRNA sequencing of  rainbow trout: Celliobose supplementation
PCR amplicons from the V4 region of 16S rRNA genes were generated using specific target primers (515F and 806R). PCR reactions were carried out with Phusion High-Fidelity PCR Master Mix with GC Buffer (New England Biolabs). The library was constructed by NEBNext Ultra II DNA Library Prep Kit and then sequenced after quality check . Paired-end reads were assigned to samples based on their unique barcodes and truncated by cutting off the barcode and primer sequences. After the barcode sequence and primer sequence were truncated, FLASH  was used to merge the reads to get raw tags.