16S amplicon sequencing of fecal bacteria in FOXA knocokout mice: DKO_R8
DNA was extracted from samples using the DNEasy PowerSoil Pro Kit (Qiagen) according to the manufacturer's protocol. Barcoded PCR primers annealing to the V1-V2 region of the 16S rRNA gene was used for library generation. The resulting library was sequenced on the Illumina MiSeq using 2x250 bp chemistry.