small RNA sequencing of Aedes aegypti cells infected with RVFV
For total RNA isolation, four wells per construct were combined using 1 mL TRIzol (Thermo Fisher Scientific) and glycogen as carrier; following the manufacturers protocol.Small RNA sequencing was performed at BMKGene (Mnster) on an Illumina Novaseq SP with SE50 and 10 M reads/sample. Therefore, at least 1.5 g total RNA was sent to BMKGene for the library preparation with VAHTS Small RNA Library Prep Kit for Illumina (Vazymebiontech) according to the manufactures protocol.