RNA-seq of C.crenata cv. Porotan fruits in early stage rep3
Total RNA was extracted from small fractions mainly containing pellicles of samples harvested in second week of July using an Easy Spin RNA Extraction Kit (iNtRON Biotechnology) with the manufacturer's instructions. Total RNA was prepared for RNA-seq libraries with three biological replicates, which indicates harvested from individual trees. Total RNA from each sample were further processed with TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, Inc., San Diego, CA) by manufacturers manual. In brief, cDNA was synthesized and subjected to end repair, single A addition and adapter ligation. The libraries were purified and enriched via PCR amplification. Then, libraries were sequenced with HiSeq system to produce mRNA-seq data.