16SrRNA of particle-attached  bacterioplankton of Chaetoceros curvisetus: microbial assemblages
Approximately 100ml of Chaetoceros curvisetus culture was filtered on board through a 0.22-m pore-sized membrane (47-mm diameter polycarbonate, Millipore, Bedford, MA, USA). The filters were then frozen at 80C until further microbial analysis.