Anopheles stephensi ddRAD-seq library
Genomic DNA was enzymatically fragmented using SbfI and EcoRI restriction enzymes, and Illumina TruSeq compatible barcodes were ligated to the sticky ends generated for demultiplexing purposes. The libraries were quantified, pooled in equimolar concentrations, and the multiplexed library was sequenced on Illumina HiSeq X using single-end 150 bp chemistry.