Illumina genome sequence of Myxococcus xanthus DK1050
The integrity and purity of genomic DNA was examined by 1% agarose gel electrophoresis. The quality/quantity of genomic DNA was assessed using Nanodrop (ThermoFisher) and Qubit3TM (Invitrogen). The DNA of each sample was randomly sheared into short fragments of about 350 bp. The obtained fragments were subjected to library construction using the NEBNext DNA Library Prep Kit, following the instructions strictly. Briefly, as followed by end repairing, dA-tailing, and further ligation with NEBNext adapter, the required fragments (in 300-500 bp size) were PCR enriched by P5 and indexed P7 oligos. After purification and subsequent quality check, the library was ready for sequencing on Illumina sequencing platform, with the read length of PE150 bp at each end.