scRNA-seq library of musculus: adult lung
Sequencing libraries were generated using 10x Genomics Chromium Next GEM Single Cell 5 Reagent kit v2 (Dual Index) following manufacturers instructions (CG000330 Rev D). ADT-labelled (BioLegend, Cat. No. 155861, 155863 and 199903) cells were loaded onto the Chromium iX (10x Genomics) at a concentration of ~1 x 106 cells/mL, with 50,000 cells loaded per channel. One channer was loaded per 2 mice lung samples. Library generation was performed using Biomek FXP Laboratory Automation Workstation (Beckman Coulter) at MRC Weatherall Institute of Molecular Medicine Single-Cell Facility (WIMM, University of Oxford). Library quality and concentration was assessed using a Bioanalyzer (Agilent) and Qubit 2.0 Fluorometer (Thermo Fisher Scientific), respectively. Libraries were sequenced on an Illumina NovaSeq 6000 to a mean depth of 40,000 read pairs/cell for scRNA-seq, 10,000 read pairs/cell for Cite-seq and TCR-seq, performed at Novogene.