16S rRNA sequencing of  rainbow trout: Celliobose supplementation
PCR amplicons from the V4 region of 16S rRNA genes were generated  using specific target primers (515F and 806R). PCR reactions were carried out with Phusion High-Fidelity PCR Master Mix with GC Buffer (New England Biolabs). The library was constructed by NEBNext Ultra II DNA Library Prep Kit and then sequenced after quality check .