tCUT&Tag of bHLH162
The tCUT&Tag libraries were constructed were constructed using Hyperactive In-Situ ChIP Library Prep Kit for Illumina (pG-Tn5) (TD901). The samples with transfection efficiency of no less than 60% were selected for following tCUT&Tag experiment. The tCUT&Tag datasets had 2 replicates for each TF. Chromatin extracts were immunoprecipitated with anti-GFP antibody (Invitrogen, no. A-11122, 1:80 dilution). The tCUT&Tag reaction with IgG antibody was used as a control.