Consensus Genome Assembling of SARS-CoV-2: from Homo sapiens
The sequencing for SARS-CoV-2 is performed using ARTIC-NEB: SARS-CoV-2 Library Preparation v4 protocol. Briefly, the ARTIC protocol is used to attain the amplicon from the extracted RNA, followed by FS DNA library preparation kit to make the amplicon library, that is loaded onto the Illumina iSeq100 platform for sequencing at Infectious Diseases Research Laboratory (IDRL), Department of Pediatrics and Child Health, The Aga Khan University, Karachi, Pakistan. The consensus genomes are generated by IDSeq miniWDL SARS-CoV-2 workflow.