Rhodococcus pyridinivorans  Genomes
Experimental process. After obtaining qualified high-quality DNA, sequencing was performed using Oxford Nanopore Technologies' library building kitThe specific steps for library construction are as follows: Firstly, magnetic beads are used to screen large fragments of DNA; Then carry out library construction: a) After selecting magnetic beads from each libraryApproximately 1ug of DNA for damage repair and end repair; b) Purification and recovery of repaired DNA using magnetic bead method, Qubit determination of concentration and calculation of recoveryRate; c) Add the corresponding barcode to each sample; And magnetic bead purification was performed on the barcode connecting product; d) Connect multiple barcodes for productionCalculate the pooling ratio based on the demand for data volume, and pool according to the ratio; e) Splice connection, so that each DNA fragment carries recognizableSpecific splice sequence; f) Purify the connected sample DNA using magnetic bead method, measure the concentration with Qubit, and calculate the recovery rate. Finally, perform machine sequencing.