RRBS of rattus norvegicus: hippocampus with prenatal dexamethasone
DNA spiked with lambda DNA was digested with MspI, and  subjected to size selection and bisulfite conversion. A total amount of 0.8 g of DNA per sample was used as input material for conversion and library preparation. Libraries were prepared using the EZ DNA Methylation Gold Kit. Quality-controlled and quantified libraries, (using Agilent Bioanalyzer 2100, Qubit and qPCR method) were subjected to sequencing on the Illumina NovaSeq6000 system (PE150; 30 M reads per sample).