Whole genome resequencing of cowpea (Vigna unguiculata L. Walp)-parental material of Nanopore data
The DNA (1.5g) from the sample were end-repaired (NEBnext ultra II end repair kit) and purified using 1x AmPure beads. Adapter ligation (AMX) was performed at RT (20 C) for 20 minutes using NEB Quick T4 DNA Ligase. The reaction mixture was purified using 0.6X AmPure beads  and sequencing library was eluted in 15 l of elution buffer provided in the ligation sequencing kit (SQK-LSK109) from Oxford Nanopore Technology