IDT xGEN Exome Research Panel v2: Sample HCI034_pdx_P2_XXX_WXS_18252X5
Genomic DNA libraries were constructed using high molecular weight DNA (100-200 ng) using the Illumina DNA Prep with Enrichment kit (cat#20025523).  PCR-amplified libraries (500 ng) were enriched for exonic regions by hybridization with the IDT xGEN Exome Research Panel v2 (cat#10005152).  Enriched libraries were qualified on an Agilent Technologies 4150 TapeStation using a D1000 ScreenTape assay (cat# 5067-5582 and 5067-5583).  The molarity of adapter-modified molecules was defined by quantitative PCR using the Kapa Biosystems Kapa Library Quant Kit (cat#KK4824).  Individual libraries were normalized to 5 nM in preparation for Illumina sequence analysis.