Deep sequencing of engineered mutagenesis in E.coli: T7RNAP fused to rAPOBEC1 and T7RNAP fused to TadA8, no IPTG, C block reporter, Replicate: Rep2
Plasmids (BACs) were extracted from pooled clones using the Omega midiprep kit. Samples were then prepared for sequencing using Nextera/FLEX kit