Single cell RNA-seq of adult human head and neck adenoid cystic carcinoma: Sample ACC5_Primary
Individual cells from this fresh human tumor specimen were sorted into 96-well plates and lysed. Single cell RNA-sequencing was performed using the SMART-Seq2 protocol (Picelli et al., 2014). Reverse transcription was performed using either Superscript II (ThermoFisher Scientific) or Maxima (ThermoFisher Scientific) reverse transcriptase. cDNA libraries were tagmented using the Nextera XT Library Prep Kit (Illumina). 384 samples were pooled and sequenced as paired-end 38 base reads.