AMPLICON 16S Sequencing of peat metagenome
SAMPLE COLLECTION: ~12 ml of LifeGuard buffer (Qiagen) (LG) was added to the tube, followed by vigorous hand shaking to achieve a thorough mixture of LG and peat. NUCLEIC ACID EXTRACTION: PowerSoil Total RNA Isolation kit and DNA Elution Accessory kit (RC) (Qiagen Cat# 12866-25 and 12867-25).