16S rDNA adult pig feces
Bacterial V3-V4 region of 16S ribosomal DNA (rRNA) and the archaeal 16S rRNA were amplified with primers (V3_F357_N / V4_R805; Arch349F / Arch806R) described in primer Table. Amplicons were generated using a Fluidigm Access Array followed by high-throughput sequencing on an Illumina MiSeq system (Illumina, USA) performed at the Carver Biotechnology Center of the University of Illinois (Urbana, USA).