Genome-wide CRISPR activation screen in SK-N-DZ neuroblastoma cells: guide RNA distribution following RSL3 treatment (300 nM), replicate 2
Genome-wide CRISPR activation screen in SK-N-DZ neuroblastoma cells. Guide RNA distribution of the Calabrese library in RSL3-treated cells (300 nM); replicate 2. SK-N-DZ cells stably expressing dCas9-VP64 were transduced with the Calabrese library (Set A) at a multiplicity of infection of 0.3. After puromycin selection and recovery, cells were collected, genomic DNA extracted and amplicon libraries constructed for Illumina sequencing.