RNA-seq of Ixodes scapularis adult female salivary glands during feeding on rabbits
Intact salivary glands (SG) from unfed Ixodes scapularis adult females were collected and stored in RNAlater solution. The average weight of the group unfed is 1.6 mg. RNA extraction was performed with the AllPrep DNA/RNA/Protein Mini Kit (QIAGEN) according to manufacturer`s specifications and analyzed with an TapeStation system (Agilent, USA). The library was constructed using the NEBNextUltraTM II (Directional) RNA with polyA selection library prep kit and sequencing was performed in an Illumina Novaseq 6000 DNA sequencer.