RNA-seq of suman primary VSMC: control calcification+10uM Cu, 9 days, replica 2
Libraries were built using a Illumina Stranded mRNA library Preparation Kit (Illumina, USA) following the manufacturers protocol. Quality control was performed on an Agilent BioAnalyzer. Sequencing was performed on a NextSeq2000 (Illumina) to obtain 65 bases single-end reads.