CUT&RUN of Ubn1/2 mutant ESCs for H3K4Me3
CUT&RUN was performed using Cell Signaling CUT&RUN Assay Kit #86652, then DNA was purified using Cell Signaling DNA Purification Buffers and Spin Columns #14209. For library prep, NEBNext Ultra II DNA Library Prep Kit for Illumina was used. The antibody used was Cell Signaling Mono-Methyl-Histone H3 (Lys4) (D1A9) XP® Rabbit mAb #5326.