Dual RNA-seq of T. castaneum infected with B. bassiana
Total RNA (fungus + insect) was extracted from pools of ten insects fed a conidia-added diet for 3, 12, 48 and 72 h employing the RNAeasy Plant Mini kit (Qiagen, Germany), which include an on-column DNA digestion step (Qiagen, Germany). Total RNA was quantified with a Nanodrop spectrophotometer (Thermo, USA), and the integrity was assessed on a 1% (w/v) agarose gel. For all samples the obtained RIN was > 7.0, as is needed to follow with the protocol below.