CRISPR/Cas9 mutation readout on the pathway reporters in K562 TRIP RSTP2_clone5 on sample: K562_ddCas9_RSTP2_clone5_robotics_screen_P2_F2_FEN1_LBR2_72h_B5_R1_Run2
CRISPR/Cas9 mutation readout on the pathway reporters in K562 TRIP RSTP2_clone5 after individual DNA repair protein knock-outs. Five days after knock-out induction, a second gRNA is transfected to induce DSB in 19 pathway reporters in parallel. Three days later, cells are harvested for mutation readout PCR.