RNA seq raw data of Akt1/2/3 KO FC1245 murine pancreatic cancer cells
RNAs were extracted from cell culture and sent to Novo Gene for library construction and sequencing. Briefly, messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.