Targeted PacBio Sequencing of NLR Exons from Zebrafish
Genomic samples were sheared with Covaris Red miniTUBEs. Multiplexing adapters were added with Illumina NEBNext Ultra II, followed by targeted capture with RNA baits, amplification with PCR, AMPure XP Beads size selection, PacBio library preparation and a final size selection with BluePippin.