RNA-Seq of Mycobacteroides abscessus treated with MXF
Total RNA was isolated using RNeasy Plus Mini Kit (Qiagen, USA) with Lysing Matrix B (MP biomedicals, USA) and residual DNA was removed by treatment with DNase. The quantity and purity of the sample were checked by RNA ScreenTape Analysis (Agilent, USA) and the amount of ribosomal RNA in the sample was minimized using NEBNext rRNA Depletion Kit (New England Biolabs, USA). Complementary DNA library was synthesized using the TruSeq Stranded Total RNA Library Prep Gold Kit (Illumina, USA), and then quality of the produced library was checked by DNA ScreenTape Analysis (Agilent, USA). Paired-end reads of 2  100 bp were generated using the NovaSeq 6000 S4 Reagent Kit version 1.5 (Illumina, USA) and the NovaSeq 6000 instrument (Illumina, USA).