Genome of Ligilactobacillus salivarius
Genomic DNA was extracted from 5 mL of culture with a first step of enzymatic lysis with the following cocktail: mutanolysin at 233.3 U/mL; lysostaphin 13.3 at U/mL and lysozyme at 50 251 mg/mL. Following incubations with RNAse A (Qiagen) at 10 mg/mL and proteinase K 252 (Euromedex, France) at 50 mg/mL. Purification was performed with a DNA extraction kit 253 (Genomic DNA Buffer Set and Genomic Tips, Qiagen), according to manufacturers instructions. DNA was resuspended in TE buffer and concentration was measured with NanoDrop (NanoDrop 1000, ThermoFisher). Genome was sequenced by Eurofins Genomics (France) by whole-genome sequencing with de novo assembly, with PacBio method on SMRT  (Single Molecule Real Time) cell.