Multimarker eukaryotic metabarcoding Analysis of water from AWCC pond
1 Liter of water from a pond at the Commonwealth of Virginias Aquatic Wildlife Conservation Center was passed through a 0.45 micron filter. DNA was extracted from the microbes caught on the filter using Qiagens Power Water kit. 18S, algal 28S, fungal 18S, and diatom rbcL  barcodes were PCR amplified. Illumina sequencing, including library preparation, was completed by Azenta (South Plainfield, NJ, USA).