Metatranscriptomics of WWTP islet
1g of RNA was rRNA depleted using the RiboZero kit (Illumina, MRZB12424). rRNA depleted samples were further processed and prepared using TruSeq Stranded mRNA library preparation kit (Illumina, RS-122-2101). The fragmentation time was reduced to 3min. The samples were amplified for 8 PCR cycles. The prepared libraries were quantified using Qubit (ThermoFischer) and quality checked using Bianaoyzer 2100 (Agilent).