Hyb-Seq of Carex rostrata Stokes
Baits were previously designed in Villaverde et al. (2020). Sequencing libraries were prepared using the Illumina TruSeq Nano HT DNA kit (Illumina Inc., San Diego, CA, USA). DNA libraries were checked for quality using the 2100 Bioanalyzer system (Agilent, Santa Clara, CA, USA), and library concentrations were quantified using a Qubit 2.0 Fluorometer (Life Technologies, Grand Island, NY, USA). De-multiplexed sequences were quality trimmed using Trimmomatic (Bolger et al., 2014).