16S amplicon sequencing of soil sample impacted by spatial allocation of soil water and soil organic carbon
V3-V4 region of 16S rRNA gene amplified with Bacteria-specific primer pair having Illumina-compatible adapters on their 5' ends (Bakt_341F: 5-CCTACGGGNGGCWGCAG -3 and Bakt_805R: 5-GACTACNVGGGTATCTAATCC-87 3) (Herlemann et al., 2011)