RNA-seq of crab eyestalks
After the removal of low-quality sequences and adapter sequences, the clean reads were then de novo assembled using Trinity to obtain a reference sequence for subsequent analysis. The concordance between the RNA-seq data corresponding to the two groups was assessed using two methods, namely principal component analysis (PCA) and pairwise correlation analysis. Subsequently, blast alignment against NR (non-redundant) (https://ftp.ncbi.nlm.nih.gov/blast/db/FASTA/), PFAM (protein family) (http://pfam.xfam.org/), Swiss-Prot (protein sequences) (http://www.gpmaw.com/html/swiss-prot.html), KEGG (Kyoto Encyclopedia of Genes and Genomes) (https://www.kegg.jp/), KO (KEGG orthologue database) (https://www.genome.jp/kegg/ko.html), and GO (Gene Ontology) (http://geneontology.org/), eggNOG (evolutionary genealogy of genes: Non-supervised Orthologous Groups) (http://eggnog5.embl.de/) was performed.