Illumina TruSeq DNA library from Royle pastel (b/b), Neovison vison DNA (mink_rp3) sequenced on Illumina NovaSeq 6000 with NovaSeq S4 Reagent Kit v1.5 (300 cycles)
Genomic DNA from mink tissues was extracted using QIAGEN Mini Spin Columns, following the manufacturers protocol (QIAGEN, Germany). Two samples, each containing  2,500 ng of Royal pastel mink DNA, were dephosphorylated with FastAP Thermosensitive Alkaline Phosphatase (Thermo Scientific, USA) and cleavage with Cas9 (Biolabmix) protein using one pool of gRNA per sample. Cleavaged DNA were purified, mixed and used for Nanopore library construction with Ligation Sequencing Kit (#SQK-LSK109 Oxford Nanopore Technologies, United Kingdom) and NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing (NEB, USA) following the manufacturers protocols. Constructed library were sequenced on single FLO-MIN106 R9.4.1 flow cell (Oxford Nanopore Technologies) on GridION sequencer at Sirius University (Sirius, Russia). We used adaptive sampling application during sequencing, NC_058096.1:75,098,354-75,178,512 region of mink genome was sleeted as target.