PROseq of RHPS4-treated fibroblasts
Design: PRO-seq library generated from neonatal foreskin fibroblasts. Cells were treated with 1 uM RHPS4 or vehicle for 24 hours; Drosophila S2 cell spike-ins were added. Biotin-labelled nascent RNA converted to cDNA and the product was amplified 22 cycles. Products between 150-300 bp (insert >70 bp) were PAGE purified then sequenced by the Illumina HiSeq 2500 instrument to a depth of >150 million reads.