Illumina WGS of Borrelia burgdorferi: isolated from Ixodes pacificus in Western Canada
DNA was extracted from Borrelia burgdorferi culture aliquot using the DNeasy UltraClean Microbial kit. Sequencing library was then prepared using the Illumina DNA Prep kit. DNA input  was approximately 1 ng, requiring qPCR quanitifcation with NEBNext Library Quant Kit for Illumina enabling manual library normalization prior to sequencing/loading.