nanopore WGS data for A. lentis isolate Kewell called with Guppy 5.0.7 and the super accurate model
DNA extraction for long-read sequencing was performed as described by Debler et al. 2020 and in detail on protocols.io (https://dx.doi.org/10.17504/protocols.io.bgw5jxg6). Briefly, the isolate was grown in yeast extract dextrose liquid media with constant shaking at 180 rpm for 72 h at 22 C. Mycelium samples were frozen with liquid nitrogen and ground to a fine powder. DNA was extracted from approximately 500 mg of powdered mycelia using a modified cetyltrimethylammonium bromide (CTAB) method. DNA concentration and purity were determined using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and NanoDrop Spectrophotometer (Thermo Fischer Scientific, Waltham, MA, USA). DNA integrity was assessed on a 1.5% TBE gel for 60 h using the 5-80 kb protocol on the Pippin Pulse (Sage Science, Beverly, MA, USA). Libraries were prepared using the SQK-LSK109 ligation sequencing kit and sequenced on the MinIONTM nanopore platform