ChIRP-seq of Plasmodium falciparum targeting Lnc-ch14, gametocyte stage
Libraries from the ChIRP samples (RNA pulled down by specific probes, converted to cDNA) were prepared using the KAPA Library Preparation Kit (KAPA Biosystems). Libraries were amplified for a total of 12 PCR cycles (12 cycles of [15 s at 98C, 30 s at 55C, 30 s at 62C]) using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems).