Illumina amplicon next generation sequencing of Dirofilaria immitis: sample HW53_L21554
First stage PCRs for Illumina Next Generation Sequencing (NGS) were performed in-house according to the 16S Metagenomic Sequencing Library Preparation instructions for the Illumina MiSeq system (Illumina, Australia). PCR products (25 L) were submitted to the Ramaciotti Centre for Genomics, The University of New South Wales, Australia for indexing (384), library preparation and NGS using Illumina MiSeq v2 250PE sequencing run.