DNA-Seq of RTPR amplicons from viral size fraction of seawater
DNA was extracted from the 0.02 m Anotop filters (virioplankton fraction) and quantified. RTPR PCR amplicons of ~750 bp were obtained.  After magnetic bead purification, amplicon molecules were tagged with a unique barcode sequence using ligation.  Purified, barcoded amplicons were then subjected to a limited cycle PCR with barcode sequences as template targets.  Enriched barcoded amplicons from each sample were then purified, pooled in equal proportions, and sequenced using a PacBio RSII sequencer