Bulk Microglia RNA-seq: repetitive close head injury (rCHI) Normal Diet (ND) Biological Replicate #1
Briefly, 2,000 isolated CD45 low to intermediate/CD11b high cells (microglia) were lysed in 5ul TCL buffer + 1% -mercaptoethanol. Preparation for RNA libraries for sequencing was performed using standard Illumina protocols.