Illumina RNA-seq of zebrafish: fertilized egg replicate4
mRNA from five stages (fertilized egg, 1-cell, 64-cell, 1k-cell, and shield) was extracted with Dynabeads@ mRNA Purification Kit (Ambion) and subjected to TURBOTM DNase (lnvitrogen) treatment. RNA-Seq libraries were prepared using the NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) and then sequenced at 275 bp paired-end mode on an Illumina HiSeq X Ten system with 8 replicates for each stage.