AMPLICON 16S Sequencing of peat metagenome
SAMPLE COLLECTION: ~12 ml of LifeGuard buffer (Qiagen) (LG) was added to the tube, followed by vigorous hand shaking to achieve a thorough mixture of LG and peat. NUCLEIC ACID EXTRACTION: PowerMax Soil DNA Isolation kit - high humic acid protocol (PSH) (Qiagen Cat# 12988-10HH)