RNA-Seq of Ptolemba bulliensis
Multiple individual cells were isolated directly from a previously established monoclonal culture. We extracted RNA from these cells and synthesized double-stranded cDNA (dscDNA) using a modified version of Smart-seq2 protocol (Piceli et al. 2014) that includes additional freeze-thaw steps to improve cell lysis as described in Onsbring et al. (2020). Following the Smart-seq2 protocol, we prepared libraries to be sequenced on Illumina platform using a Nextera XT DNA Library Prep Kit (Illumina, CA).