Metagenomics of WWTP islet
400 ng of DNA was sheared using NGS Bioruptor (Diogenode, UCD300) with 30s ON and 30s OFF for 10 cycles. DNA libraries were prepared using TruSeq Nano DNA kit (Illumina, FC-121-4002) using standard protocol with 8 PCR cycles. The libraries were prepared for 350bp average insert size.