R-R2
Different tissues like stem(ST), root (R) and three different stages of leaves (L-ZYM, M-ZYM, S-ZYM), from 3-4 month old plant (Monarda citriodora) were used as a source material for RNA isolation  (two biological replicates of each sample) using Zymo Research Quick-RNA Microprep Kit (according to manufacturers protocol). The isolated RNA samples were then treated with DNaseI (Fermentas, Burlington, Canada) to remove genomic DNA.  The quality of the RNA starting material RIN value was  6 and starting input concentration of RNA was ~ 1ug. RNA seq libraries were prepared using TruSeq Stranded RNA Library prep Kit with Plant Ribozero (Illumina Inc., USA) following manufacturers instructions. Libraries were qualified using Agilent Tapestation 2200 and quantified using Qubit 3.0 fluorometer and the validated libraries were sequenced on Illumina Hiseq 4000 / NovaSeq 6000 Platform. Sequencing of each sample were done in two technical replicates.