Genome sequencing of Lassa mammarenavirus from Mastomys natalensis lung sample, animal 00106
Nucleic acids were extracted using Applied Biosystems MagMAX CORE and Pathogen kits with a KingFisher Duo Prime purification system. The nucleic acids were treated with Ambion DNAse I, and RNA was purified using Beckman Coulter RNAClean XP and depleted of rRNA using an Illumina Ribo-Zero Plus kit. cDNA and sequencing libraries were generated and sequenced on an Illumina MiSeq.