Raw reads of Heterosigma
Poly-A selected RNA libraries were prepared using the NEXTFLEX Rapid Directional RNA-seq kit 2.0 (Perkin Elmer, Waltham, MA) as per the manufacturers instructions. Each library was prepared with a unique barcode and pooled at equimolar concentrations. The pooled samples were sequenced on an Illumina HiSeq. 2500 (Illumina, San Diego, CA) at a read length of 2150bp, targeting 60 million read pairs per sample.