ddRADseq of Rana sphenocephala adult female specimen
We prepared DNA libraries using a double-digest restriction site-associated DNA (ddRAD). We used the restriction enzymes SbfI-HF and MspI. We sequenced libraries on an Illumina HiSeq 4000 with 150 base pair paired-end sequencing at the Ohio State University Comprehensive Cancer Center Genomics Shared Resource.