CLIP-seq NUP153(1-650)-eGFP HEK23T infected TBEV Rep2
CLIP-seq of NUP153(1-650)-eGFP bound RNA following GFP immunoprecipitation. HEK293T cell where transfected with NUP153(1-650)-eGFP and infected with TBEV (oTro  strain). Following this immunoprecipitation using GFP-trap beads was performed and RNA was digested with RNAse A. Bound RNA was eluted and size was verified using Bioanalyzer. The NEBNext small RNA sequencing kit was  used to a prepare cDNA library's. Samples where sequence using the MiSeq platform.