Glass-3D+BMP 0.5-hr replicate 2
The quality of total RNA was determined using TapeStation RNA ScreenTape. RNA was enriched for mRNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490). cDNA and library preparation were performed using NEBNext Ultra II Directional RNA Library Prep kit for Illumina (New England Biolabs). Library concentration was determined using NEBNext® Library Quant Kit for Illumina (New England Biolabs) or by Qubit (Thermo). Samples were then pooled and were sequenced.