RNA-Seq of male Jacana spinosa Whole brain tissue
Libraries were prepared by Illumina TruSeq Stranded mRNA Library Preparation Kit protocol (catalog no. 20020595).  The libraries were analyzed by Agilent 4200 TapeStation, and pooled.  The pooled libraries were loaded on a NextSeq 500/550 High Output v2.5 run with a 75-cycle sequencing module (catalog no. 20024906) to generate paired-end reads.  The demultiplexing of the reads was performed using bcl2fastq version 2.20.0.