WGS of Mycobacteroides abscessus treated with MXF
Genomic DNA was isolated using Quick-DNA Fungal/Bacterial Kits (Zymo, USA), and the size and quantity of DNA was checked by Genomic DNA ScreenTape Analysis (Agilent, USA). DNA library was constructed using the TruSeq Nano DNA Kit (Illumina, USA) and the quality of the library was checked by DNA ScreenTape Analysis (Agilent, USA). To generate sequence data from the validated library, the Hiseq X ten Reagent Kit version 2.5 (Illumina, USA) and the Hiseq X ten instrument (Illumina, USA) were used to obtain paired-end reads of 2  150 bp.