MiFish 12S Metabarcoding of Ethanol Preservative: CalCOFI ichthyoplankton samples
Up to 125 mL of Ethanol Preservative from archived CalCOFI ichthyoplankton samples were filtered on 0.2 micron PVDF filters, amplified with MiFish 12S primers, Nextera unique dual indices, sequenced on NextSeq PE midoutput