RNA-seq for MDS patients (control samples
Lymphoblast cells from MECP2 duplication syndrome patients were cultured at basal conditions until confluent. Ten million cells were pelleted and frozen until processing. One half pellet was processed for total RNA using the Qiagen RNeasy kit. Total RNA was processed for RNA sequencing after polyA selection. The same procedure was followed using lymphoblast cells from unaffected control individuals, obtained from Coriell.