TWO-STAGE ANAEROBIC CO-DIGESTION OF WASTES FROM THE CITRUS AGROINDUSTRIAL: OPTIMIZATION OF METHANE PRODUCTION FROM HYDROGEN FERMENTATION EFLUENT
Genomic DNA from the microbial community of the samples was extracted using the FastDNA SPIN Kit for Soil DNA Extraction (MP Biomedicals) according to the manufacturer's recommendations. The quantification (ng/L) and the purity (260/280nm ratio) of the DNA extracted from the samples was analyzed in Nanodrop 2000 Spectrophotometers (ThermoFisher Scientific), and the integrity was verified by electrophoresis in 0.8% agarose gel. DNA samples were sent to ByMyCell (Ribeiro Preto, SP - Brazil) for sequencing of the 16S rRNA gene (V3+V4 region) and basic bioinformatics analysis for taxonomic identification. Amplification of the 16S rRNA gene was performed with a set of 341F/806R primers (Youngseo et al., 2005). The resulting fragments were submitted to sequencing on an Illumina PE 250 platform.