Probe capture of Influenza A virus
Total RNA isolation using the MagNA Pure 96 DNA and Viral NA Small Volume Kit . Quantification of viral RNA using a RT-qPCR targeting the Influenza A virus matrix gene. Viral RNA enrichment and preparation using the Illumina RNA Prep and Enrichment with the Respiratory Viral Oligo Panel. Sequencing of multiplexed libraries using the Illumina MiniSeq platform. Bionformatic analysis of paired-end raw sequence reads.