PacBio Sequel II genome sequencing of spore-forming community isolate JLR.KK009
The DNA was made into SMRTbell libraries using the Express Template Prep Kit 2.0 from PacBio according to the manufacturers protocol, pooled into a single multiplexed library and size selected with Sage Sciences' BluePippin  with a size selection cutoff of 8000 (BPstart, value). The size-selected SMRTbell library was annealed and bound according to the SMRT Link Set Up, and sequenced on a Sequel II.