GBS of barley: cv Vorsinsky-2
Total genomic DNA was extracted from young leaf tissue with the DNEasy Plant Mini Kit (Qiagen Inc., Valencia, CA, USA). The concentration and purity of the tested samples were determined by gel electrophoresis and on a NanoDrop 2000 Spectrophotometer (Thermo Scientific, USA). Genomic DNA was digested with restriction enzymes MspI (C^CGG) and PstI (CTGCA^G) (NEB, Ipswich, MA, USA). Sequencing was performed on an Illumina NextSeq 550 platform by a 150 bp single-ended protocol.