RNAseq of cultured skin fibroblasts from a control subject
RNA was extracted from CMT2AMFN2 fibroblasts and controls fibroblasts using the RNeasy Plus Kit (Qiagen, 74034) according to the manufacturers instructions. Each RNA sample was qualitatively and quantitatively checked on Agilent 2100 Bioanalyzer RNA Nano Chip (Agilent, Santa Clara, CA, USA). Directional RNA-Seq libraries were prepared from 100 ng of total RNA using the TruSeq Stranded Total RNA Sample Prep Kit (Illumina, 20020597) according to the manufacturers protocol. Sequencing was performed on an Illumina NextSeq 500 platform (Illumina, San Diego, CA).