RNA-Seq of mus musculus: adult male liver
Total RNA was extracted using the TRIzol reagent (Invitrogen, CA, USA) accordingtothemanufacturers protocol. RNA purity and quantification were evaluated using the NanoDrop2000spectrophotometer (Thermo Scientific, USA). RNA integrity was assessed usingtheAgilent2100Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Then the libraries were constructedusingVAHTS Universal V6 RNA-seq Library Prep Kit according to the manufacturers instructions. Thetranscriptome sequencing and analysis were conducted by OE Biotech Co., Ltd. (Shanghai, China)