INSeq of Akkermansia: in vitro mucin
INSeq analysis of Akkermansia muciniphila genes required for growth in mucin in vitro. A pooled library of A. muciniphila transposon mutants was grown with mucin as a carbon and nitrogen source. DNA was purified from the cultures and INSeq/Tn-Seq libraries were prepared as described in Goodman et al. (PMID: 22094732).