RNA-seq of Ocellarnaca sp: adult female
polyadenylated mRNA was isolated from total RNA using Oligo (dT) magnetic beads and then fragmented randomly by Fragmentation Buffer. Using fragmented mRNA as template, the first strand cDNAs were synthesized by random hexamer primers, and the second strand cDNAs were synthesised by DNA polymerase I (New England BioLabs) and RNase H (Invitrogen). After end repair, A-tail, adaptor ligation and purified with AMPure XP beads, PCR amplification was conducted.