AMPLICON 16S Sequencing of peat metagenome
SAMPLE COLLECTION: ~12 ml of LifeGuard buffer (Qiagen) (LG) was added to the tube, followed by vigorous hand shaking to achieve a thorough mixture of LG and peat. NUCLEIC ACID EXTRACTION: Standard with LG spin down (SL) - Identical to the 'S' method, except that peat materials stored in LG were centrifuged at 4,700 xg for 5 min to separate peat from LG, which was removed before adding the peat materials to the extraction process.