The Influence of Substrate Type and Incubation Environment on the Microbial Community Composition of Erie, PA Sour Dough Bread Starter Cultures
Samples were analyzed using Zymo Researchs Quick-ITS kit for ITS2. Phased primers ITS3f (GCATCGATGAAGAACGCAGC) and ITS4r (TCCTCCGCTTATTGATATGC) were used to generate a 2x300 bp of the internal transcribed spacer 2 (ITS2) region. Sequencing was then performed using manufacturer guidelines with a P1 600cyc Nextseq2000.