Whole Genome Sequencing of HeLa cells after MNase treatment
Briefly, nuclei were embedded in LMP agarose and lysed with 1% Triton X-100 in PBS/EDTA. After lysis, the chromatin was treated with Micrococcal Nuclease. After the treatment the sample was sonicated with a Diagenode Bioruptor to generate 200-1000 bp fragments. The DNA was purified (Agarose DNA extraction Kit), then quantified with a Qubit fluorometer. ChIP-seq libraries were prepared by Illumina according to manufacturers instructions.