MinIon sequencing of gDNA from  salivary glands of In(1)sc[8], In(1)19EHet, Rif1[1] flies
DNA isolation by proteinase K treatment and phenol-chlorophorm purification, sequencing on MinIon flowcell using Ligation Sequencing Kit (SQK-LSK109) + barcoding  NBD104 (ONT)