16s metagenomics of chicken gut  in different feeding treatment
DNA was extracted with the QIAamp DNA Stool Mini (QIAGEN, Hilden, Germany), following the manufacturers' recommendations. 16S rRNA V3/V4 region was amplified using primers 341F (5- CCTACGGGRSGCAGCAG-3') and 806R (5-GGACTACHVGGGTWTCTAAT-3), with Illumina adapters, are required for sequencing. Amplification was performed in 35 cycles at 50 C annealing temperature, tripled for each sample.