gut metagenome
Fecal samples were stored with DNA storage buffer provided by BGI and kept in -80 fridges until use. Microbial DNA was extracted from fecal samples (200 mg) using the QIAamp PowerFecal Pro DNA Kits following the protocol provided by the manufacturer. Fecal metagenomes were sequenced in high-quality fecal DNA samples. The DNA library was prepared and sequenced by the DNBSEQ-T7.