Mock control for Bio-EZH1 ChIP-seq at 28h.
The extracted 20ng ChIP-DNA (using ChIP-DNA Clean & Concentrator, Zymo) was processed for ChIP-seq high-throughput library preparation (NEBNext® Ultra™ II DNA Library Prep Kit, NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1), NEB). The ChIP libraries were purified using Ampure XP beads and were quantified using qubit dsDNA HS assay kit. ChIP-seq sequencing was performed using Novaseq6000 platform (150bp PE)