MIA PaCa-2_TRC
The total RNA of 2D cultured cells or PDAC TRCs was extracted by using TRIzol reagent (Invitrogen, CA, USA) and the quantity and purity were monitored using NanoDrop ND-1000 (NanoDrop, Wilmington, DE, USA) as well as Bioanalyzer 2100 (Agilent, CA, USA). OligodT magnetic beads (25-61005, Thermo Fisher, CA, USA) enriched mRNAs were fragmented. cDNAs were synthesized from the fragmented RNA using a Reverse Transcriptase (Invitrogen SuperScript II Reverse Transcriptase, CA, USA), and then sequenced by Illumina Novaseq 6009 (LC Bio Technology Co., Ltd. Hangzhou, China).