RNA-Seq from Posidonia oceanica seedlings
Indexed libraries were prepared from 800 ng/ea purified RNA with Illumina Stranded mRNA Prep according to the manufacturers instructions. Libraries were quantified using the TapeStation 4200 (Agilent Technologies) and and Qubit fluorometer (Invitrogen Co.), then pooled such that each index-tagged sample was present in equimolar amounts, with final concentration of the pooled samples of 5nM. The pooled samples were subject to cluster generation and sequencing using an Illumina Novaseq6000 System (Illumina) in a 2x151 paired-end format. The raw sequence files were generated in .fastq format.