OmniC Library of Crested Gecko: embryo
For each Dovetail Omni-C library, nuclear chromatin was fixed with formaldehyde and extracted. Following digestion with DNAse I, chromatin ends were repaired and ligated to biotinylated bridge adapters followed by proximity ligation of adapter- containing ends. After proximity ligation, crosslinks were reversed, and the DNA was purified. Purified DNA was treated to remove free biotin that was not incorporated into ligated DNA fragments. Sequencing libraries were generated using NEBNext Ultra enzymes and Illumina-compatible adapters. Biotin-containing fragments were isolated using streptavidin beads before PCR enrichment of each library.