Migratory and resident wood stork (Mycteria americana) whole genome sequencing
DNA was extracted from feathers or blood (stored in tubes pre-coated with the anticoagulant EDTA) using the DNeasy Blood & Tissue Kit (Qiagen). The extracted DNA samples were purified using the ZYMO DNA Clean and Concentrator Kit. The quality and concentration of the DNA samples were validated using Nanodrop. The sequencing libraties were prepared using 1 ug per sample of DNA with the xGen DNA Library Prep EZ Kit (IDT) and xGen UDI Primers Plate (IDT). The quality of the libraries was validated using the Bioanalyzer High Sensitivity DNA Assay (Agilent) before being sequenced on the NovaSeq S4 200 Lane sequencing platform (Illumina).