RNAseq of differentiating hypothalamic neurons: NCRM5-derived Day 7
RNeasy Plus Mini Kit (74136, Qiagen) according to the manufacturers instruction. Genomic DNA was eliminated by both the gDNA eliminator column and on-column incubation with DNase I (79256, Qiagen). Sequencing libraries were constructed using Illumina TruSeq Stranded mRNA kit.