RNA-Seq of M. nivale F00608 cultivated in the synthetic medium and supplemented with the plant extract
For RNA-Seq analysis, total RNA was extracted from M. nivale mycelium grown for 10 days submerged in LSM and then sustained in the presence or absence of rye extract for 24 h (for the comparative transcriptome analysis and for the identification of protein-encoding M. nivale genes), as well as from axenically grown rye plants (cultivar Ogonek) infected with M. nivale 20 days post infection (Gorshkov et al. 2020) (for the identification of the protein-encoding M. nivale genes). Fungal mycelium or plant material was ground in liquid nitrogen. Total RNA was