EVALUATION OF MICROBIOME COMPOSITION IN ORAL AND ATHEROSCLEROTIC PLAQUE
DNA was extracted using Qiagen DNeasy Powersoil Pro, integrity checked on 1% agarose gel, and concentration estimated using Qubit DNA HS kit. 300 ng of DNA was prepared for libraries using QIAseq FX DNA Library Kit. Fragmented DNA was end-repaired, ligated with Illumina-specific adapters, and amplified for sequencing. Library quality control was performed using Agilent D1000 screentape and Qubit 1X dsDNA High Sensitivity.