16S-V4 metagenomics: 10^4 CFU Salmonella-spiked caecal content DNA extracted directly replicate B
DNA extracted with DNeasy PowerSoil Pro Kit (Qiagen). V4 variable region amplified by PCR with primers 515F-806R (Caporaso 2011) and sequencing libraries were prepared according to the 16S Metagenomic Sequencing Library Preparation protocol.