Agilent SureSelect XT Human All Exon V6+COSMIC: Sample HCI030_pdx_P1_XXX_WXS_14990X21
Genomic DNA (100-3000 ng) was sheared in a 130 ul volume using a Covaris S2 Focused-ultrasonicator with the following settings: Intensity 5.0; Duty Cycle 10%25; Cycles per Burst 200; Treatment Time 6:00 minutes.  Library construction was performed using the Agilent Technologies SureSelect XT Reagent Kit (cat# G9611A) with 6 cycles of pcr.  The concentration of the amplified library was measured using a Qubit dsDNA HS Assay Kit (ThermoFisher Scientific cat# Q32851).  Amplified libraries (750 ng) were enriched for exonic regions using the Agilent Technologies SureSelect(XT) Human All Exon v6+COSMIC kit  (cat# 5190-9307) and pcr amplified (10 cycles) using Agilent Herculase II Fusion DNA polymerase (cat# 600677).  Enriched libraries were qualified on an Agilent Technologies 2200 TapeStation using a High Sensitivity D1000 ScreenTape assay (cat# 5067-5584 and 5067-5585) and the molarity of adapter-modified molecules was defined by quantitative PCR using the Kapa Biosystems Kapa Library Quant Kit (cat#KK4824).  The molarity of individual libraries were normalized to 5 nM and equal volumes were pooled in preparation for Illumina sequence analysis.