ONT sequencing of ShHELIX with gRNA TS2 targeting the chromosome (Fig. 2e-2g)
Cas9-based enrichment of target region (using ONT kit SQK-CS9109), followed by amplification-free long-read sequencing library preparation (Oxford Nanopore Technologies, SQK-LSK109) performed using a barcode expansion kit (Oxford Nanopore Technologies, NBD-104)