Douching is Associated with Dysregulated Rectal Mucosal Immunity in Sexual Minority Men
Sequencing of the V3-V4 region of the bacterial 16S rRNA gene was performed using the 515FB-806RB primer set, per Earth Microbiome Protocols. Amplified in triplicate for each sample followed by pooling of each replicate. Pooled library was sequenced using a 300-cycle Illumina MiSeq Kit, generating 150bp paired-end reads.