PacBio Iso-seq O. felineus
A total of 50 adult O. felineus flukes were mechanically homogenized in 1ml of Tri reagent (Sigma-Aldrich, USA) and extracted as per the manufacturers instructions. Finally, 2 mg of RNA was resuspended in 20 ml of DEPC H20 and submitted for RNA sequencing at the Australian Genome Research Facility