RNA-Seq of Gammarus Locusta: adult male whole body
Total RNA was extracted using the Illustra RNAspin Min RNA Isolation Kit (GE Healthcare), according to the manufacturers protocol. RNA sample integrity and concentration was initially checked by agarose gel electrophoresis and then by an Agilent Bioanalyzer 2100 (Agilent Technologies, USA). RNA-seq libraries and sequencing, Illumina HiSeq2500000 platform paired-end (2x150) were obtained commercially at Novogene Europe, Cambridge, UK.