RNA-seq of Plasmodium falciparum: gam stage, NF54 control
Libraries were prepared from extracted total RNA, first by isolating mRNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB), then using the NEBNext Ultra Directional RNA Library Prep Kit (NEB). Libraries were amplified for a total of 12 PCR cycles (12 cycles of [15 s at 98C, 30 s at 55C, 30 s at 62C]) using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems).