Nanopore sequencing of mus musculus: INTACT isolated microglia
Nuclei from specific cell types were isolated via INTACT. High molecular weight DNA was purified from isolated nuclei with the Qiagen MagAttract DNA isolation kit. Libraries were prepared with the ONT ligation sequencing kit V14 using 1 microgram of input DNA for each sample. Libraries were sequenced on the PromethION platform using R10.4.1 flow cells.