Metagenomic sequencing of Halobates melleus microbiome: adult male five individuals whole body
Metagenomic libraries were prepared using 100 ng of total genomic DNA from each sample diluted in 52.5 L of Nuclease-Free water in a 96 microTUBE-50 AFA Fiber Plate. Fragmentation of DNA to 550 bp was performed with a Covaris E220 Focused-ultrasonicator. Metagenomic libraries were prepared with the Illumina TruSeq Nano DNA Library Prep kit. Libraries were then pooled, and quality checked using a 2100 BioAnalyzer with the High Sensitivity DNA Kit and quantified via qPCR using a QuantStudioT 3 Real-Time PCR System with the KAPA Library Quantification Kit. Two sets of paired-end metagenomes were sequenced for each sample using the NovaSeq 6000 at BCL, KAUST.