DNA-Seq of Acinetobacter junii
Extracted gDNA was treated with the NEBNext Ultra II End                 Repair/dA-Tailing Module (NEB). Then barcodes from the Native Barcoding Expansion                 1-12 & 13-24 from Oxford Nanopore Technologies (ONT) were ligated using the                 NEBNext Ultra II Ligation Module (NEB). DNA was purified using Ampure XP beads                 (Beckman Coulter). The DNA from different barcoded samples was pooled and the                 adapter AMII (ONT) was ligated using the NEBNext Ultra II Ligation Module (NEB). DNA                 was purified using Ampure XP beads (Beckman Coulter), followed by sequencing with a                 R10.4 MinION Flow Cell using a MinIon Mk1B (ONT).