RNA-seq of Ixodes scapularis adult female salivary glands during feeding on rabbits
Intact salivary glands (SG) from partially fed Ixodes scapularis adult females feeding on rabbits were collected at different stages during the blood feeding and stored in RNAlater solution. Ticks were grouped by weight, the average weight of the group 6 is 105 mg. RNA extraction was performed with the AllPrep DNA/RNA/Protein Mini Kit (QIAGEN) according to manufacturer`s specifications and analyzed with an TapeStation system (Agilent, USA). The library was constructed using the NEBNextUltraTM II (Directional) RNA with polyA selection library prep kit and sequencing was performed in an Illumina Novaseq 6000 DNA sequencer.