RNA-seq of Homo sapiens: tRCC
For bulk-RNAseq library preparation, automated variant of the Illumina TruSeq™ Stranded mRNA Sample Preparation Kit was used.  This method preserves strand orientation of the RNA transcript. It uses oligo dT beads to select mRNA from the total RNA sample followed by heat fragmentation and cDNA synthesis from the RNA template.  The resultant 400bp cDNA then goes through dual-indexed library preparation: 'A' base addition, adapter ligation using P7 adapters, and PCR enrichment using P5 adapters. After enrichment the libraries were quantified using Quant-iT PicoGreen (1:200 dilution) and normalized samples were pooled and quantified using the KAPA Library Quantification Kit for Illumina Sequencing Platforms.