RNA-seq of LRRC15-TurboGFP over-expressing IMR90 E6E7 cells
RNA was extracted from cells using Bioline ISOLATE II RNA Mini Kit. Illumina Stranded mRNA Prep kit was used to prepare RNA sequencing libraries. Samples were pooled and sequenced on Illumina NextSeq 2000. Trimming, alignment and quantification of reads was conducted using Illumina BaseSpace. Human (Hg38, Alt-Masked v2, Graph Enabled) was used as reference assembly.