RNA sequencing of Colobanthus quitensis from in situ warming project
The quality and quantity of extracted RNA was assessed using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA), and a 5300 Bioanalyzer (Agilent, Santa Clara, CA), respectively. Subsequently, 2 g of extracted RNA was utilized for the synthesis of high-quality double-stranded cDNA using the Illumina TruSeq RNA Library Prep Kit v2 (Illumina, San Diego, CA) according to the manufacturers protocols. The cDNA was then subjected to end-repair, phosphorylation, and the addition of A base according to Illumina library construction protocol before being sequenced on the Illumina HiSeq 4000 platform.