MiSeq of bacteria: phyllosphere
The total genomic DNA of the phyllosphere microbes was extracted using the PowerSoil DNA Kit.  The V4 region of the bacterial 16S rRNA gene were amplified using the primer pairs 515F/806R.  Purified amplicons were pooled in equimolar concentrations and paired-end sequenced (2  300 bp) on the Illumina MiSeq PE254 platform (Illumina, San Diego, USA) according to the standard protocols at Majorbio Bio-Pharm Technology Co., Ltd. (Shanghai, China).