miRNA-seq of homo sapiens: adult male plasma
Exosome isolated from plasma using RiboTM Exosome Isolation Reagent. Exosomal RNA extracted by MagZol. Quantity and integrity assessed with Qubit2.0 and Agilent 2200 TapeStation. RNAs ligated with 3 and 16 adapters. Subsequently, the adapter-ligated RNAs were subjected to RT-PCR and amplified with a low-cycle. Products size selected by PAGE gel with NEBNext Multiplex Small RNA Library Prep Set