RNA Seq of potato: Drought Stress1
For transcriptome analysis, total RNA was extracted from potato tubers under different conditions (control and drought stress) (N=3) using Fruit-mate TM for RNA Purification (Takara Bio), and RNA iso Plus (Takara (Takara Bio) was used for RNA extraction. One milliliter of Fruit-mate TM was added to the inner medulla of the tuber, homogenized, and centrifuged at 4 C and 15,000 rpm for 5 min, and the supernatant was collected. Equal volumes of RNAiso Plus were added and homogenized. Then, 200 mL of chloroform was added, and the mixture was shaken well and centrifuged at 4 C and 15,000  g for 10 minutes. The supernatant was collected, washed with 75% ethanol, and dissolved in 20 mL of DEPC water to make samples. RNA sequencing and assembly were performed by Novogene Co. (Beijing, China). A total of more than 4.5 mg RNA from each sample was used as input material. Clean reads were obtained from the raw data by removing adapter-containing reads, poly-N-containing reads, and low-quality reads.