RNA-Seq of Fragaria vesca:receptacle
The total RNA of the samples was tested for integrity, and the mRNA was enriched with magnetic beads with Oligo (dT). mRNA was then fractioned into short fragments, and random hexamers were used to synthesize one-stranded cDNA, followed by the second-stranded cDNA. The purified double-stranded cDNA was then end-repaired and ligated into sequencing adapters, followed by fragment size selection with AMPure XP beads, and then enriched by PCR to obtain the final cDNA library.