RNA-seq of Meleagris gallopavo: adult female uterovaginal junction
Total RNA was extracted using the RNeasy Midi Kit and treated with DNase (Qiagen, Germantown, MD, USA) following the manufacturers protocol. Indexed libraries were constructed with the TruSeq Stranded mRNA Sample Preparation Kit (Illumina, Inc., San Diego, CA, USA) and size-selected for approximately 200 bp inserts. The libraries were multiplexed and sequenced on the Illumina NovaSeq 6000 platform using 150 PE flow cell to produce 51-bp paired-end reads.