INSeq of Akkermansia: in vitro mucin droplets
INSeq analysis of Akkermansia muciniphila transposon mutants grown in mucin media in microdroplets. Transposon mutants were isolated in droplets and grown for 72h. DNA was extracted using a Qiagen DNeasy Blood and Tissue DNA Extraction kit and INSeq/Tn-Seq libraries were prepared as described in Goodman et al. (PMID: 22094732).