MySeq of soil microbiome
The metagenomic DNA from each soil sample was extracted using the FastDNA spin Kit for soil from MP biomedical, following the manufacturers instruction. DNA concentration was determined by Nanodrop (Thermo Fisher) and the V3-V4 hypervariable 16s rRNA region (primers Pro341F 5-CCTACGGGNBGCASCAG -3 and Pro805R 5-GACTACNVGGGTATCTAATCC-3) was amplified. 16S rRNA library preparation was performed by BMR Genomics (Italy) for Illumina MiSeq 300PE sequencing.