Bulk RNA-seq of Adult Pigs: Pericardial White Adipose Tissue
EAT (occluded and non-occluded tissue pooled) from exercised (n=4) and sedentary (n=3) female pigs had total RNA extracted with TRIzol (Thermofisher Scientific, Waltham, MA). Total RNA was quantified (Nanodrop 3300, Thermofisher, Wilmington, DE) followed by bioanalysis (Agilent 2100 Bioanalyzer, Agilent Technologies, Inc., Santa Clara, CA) for RNA quantity and quality. RNA integrity scores fell within the following range: 6.29.1. cDNA libraries were prepared, and sequencing was performed at the Molecular Genomics Core, Texas A&M University