WGS of Phaffia rhodozyma MYM0(BPAX-A1)
DNA was extracted using the YeaStar Genomic DNA Kit (Zymoresearch Cat. No. D2002) and quantified using the Nanodrop 1000(Thermo Scientific) and Qubit dsDNA BR assay kit (Life TechnologiesCat. No. Q32850). The DNA quality was determined by running a 1%agarose gel with a DNA gel stain SYBR safe (Life Technologies Cat. No.S33102). The gel was visualized using a ChemiDoc MP system (Bio-Rad).The Illumina platform was used to sequence the genomes of nine strains(the wild-type P. rhodozyma CBS 6938 and eight mutant strains).Sequencing was performed using MiSeq V3 600 Cycle 300 PE (IlluminaCat. No. MS-102-3003). Libraries were prepared using the IlluminaNextera XT library preparation kit (Illumina Cat. No. FC-121-2003)