Cardoon-adherent microbial community 96h replicate 3
The amount of total RNA used for sequencing was 1 g. To maximize mRNA representation in themetatranscriptomic libraries, Ribo-Zero rRNA Removal Kit (Bacteria) (Illumina, San Diego, CA,USA) was used to remove rRNAs from the total RNA preparations. Library preparation wasperformed using the Illumina TruSeq Stranded Total RNA kit. Sequencing was carried out using anIllumina HiSeq2000 platform.