RNA-Seq of Crocus sativus and Fusarium oxysporum
Both Saffron and Fusarium oxysporum were eukaryotic, so magnetic beads with Oligo(dT) were used to enrich mRNA. Afterwards, cDNA duplexes were synthesized using the fragmented mRNA as a template and purified. After repairing both ends of the cDNA, add A tail and connect the sequencing adapter. After purification and elution, the PCR library was amplified. The PCR product was purified using 1.0X AMPure XP Beads to obtain the cDNA library for sequencing. After passing the quality inspection, they were sequenced on the Illumina Novaseq6000 system.