Diel analysis of a meromictic pond.
DNA was extracted from Sterivex filters using phenol chloroform (10.1038/ismej.2015.258). RNA was extracted from Sterivex filters using mirVana miRNA isolation kit (Albion), treated with DNAse using the Turbo-DNase kit (Ambion), purified and concentrated using the RNAeasy MinElute kit (Qiagen). Extracted RNA sequences were synthesized to cDNA and metatranscriptomic libraries prepared using the Nugen Ovation Ultralow Library DR multiplex system (Nugen). Both nucleic acids were sheared to 275 bp using a Covaris S-series sonicator (Woburn).