Shallow shotgun metagenomic sequence data from Sable Island horse feces
We weighed 0.16-g portions of freshly thawed fecal samples into 2-ml bead beating tubes containing Qiagen 0.7-mm dry garnet beads. Next, we added 680 l of lysis buffer (pre-warmed to 60 C) from Qiagens QIAamp 96 PowerFecal QIAcube HT Kit to each tube, and bead beat samples for 10 min on a Vortex-Genie 2 fitted with Qiagens Vortex Adapter (cat. No 13000-V1-24). All other extraction steps were completed as per the Qiagen kit QIAcube HT recommendations (using a single-tube start protocol) for high-throughput DNA extractions. Final DNA extracts were eluted in 100 l of molecular grade water. DNA extracts were quantified using Quant-iT dsDNA Broad Range Assays. Extracts were sent to the University of Calgary Centre for Health Genomics and Informatics, where shotgun metagenomic libraries were created using iGenomx Riptide preparations (1:1 mixture of low GC and High GC primers), as per manufacturer specifications.