WT_piRNA_1
The small RNA cloning was conducted. Total RNAs were extracted by Trizol (Sigma Alrich) and small RNAs were enriched by an mir-Vana miRNA isolation kit (Thermo Scientific). Samples were pretreated with homemade PIR-1 . The small RNAs were then ligated to a 3 adaptor (5 rAppAGATCGGAAGAGCACACGTCTGAACTCCAGTCA/3ddC/3; IDT) by T4 RNA ligase 2(NEB). The 5 adaptor containing 6 nt barcode was ligated using T4 RNA ligase 1. The ligated products were reverse transcribed using SuperScript III (Thermo Fisher Scientific). The cDNAs were amplified by PCR and the libraries were sequenced using the HiSeq systems (Illumina) platform at the UMass Medical School Deep Sequencing Core Facility.