16S metagenomic sequencing of human urine
Genomic DNA (gDNA) was isolated using the Quick Urine DNA Kit (Zymo Research, California, USA). Amplicons of the V3-V4 region of the bacterial 16S rRNA gene were amplified using the primers 341F and 802R. The 16S amplicon was purified using the Agencourt AMPure XP PCR Purification system (Beckman Coulter, Krefeld, Germany), processed (indexed, purified, normalized and pooled) and sequenced in a 300-bp paired-end run on the Illumina MiSeq System (Illumina, California, USA).