GSM7844500: M82 ChIP-seq_H3K36ac Rep1; Solanum lycopersicum; ChIP-Seq
ChIP-seq assays were performed on 4-week-old tomato fourth branch of leaves according to Bio-protocol of Ramirez-Prado, J.S. et al (2021) by using aAnti-RNAPII (Abcam, ab26721), Anti-H3K4ac (Active Motif, 39382), Anti-H3K4me3 H3K4me3 (Millipore 07-473), Anti-H3K4me2 (active motif, 39142), Anti-H3K4me1 (active motif, 39300), Anti-H3K9ac (Millipore, 07-352), Anti-H3K9me2 (Abcam, ab1220), Anti-H3K14ac (Millipore, 07-353), Anti-H3K14me1 (Active Motif, 61510), Anti-H3K18ac (Millipore, 07-354), Anti-H3K23me3 (Active Motif, 61500), Anti-H3K23me1 (Active Motif, 39388), Anti-H3K27ac (Abcam, ab4729), Anti-H3K27me3 (Millipore 07-449), Anti-H3K27me2 (Abcam, 24684), Anti-H3K27me1 (Millipore, 07-488), Anti-H3K36ac (Active Motif, 39380), Anti-H3K36me3 (Abcam, ab9050), Anti-H3K56ac (Active Motif, 39282), Anti-H3K56me2 (Active Motif, 39278), Anti-H3K56me1 (Active Motif, 39274), Anti-H3K79ac (Active Motif, 39566), Anti-H4K5ac (Active Motif, 39700), Anti-H4K8ac (Active Motif, 61104), Anti-H4K12ac (Active Motif, 61531), Anti-H4K16ac (Active Motif, 39930), Anti-H4K20ac (Active Motif, 61531) antibodies. ChIP-seq libraries were prepared from 10ng of DNA using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) according to manufacturer's instructions. DNA libraries were checked for quality and quantified using an Agilent 2100 Bioanalyzer (Agilent) and subjected to 1 × 75 bp high-throughput sequencing by NextSeq 500 (Illumina).