cDNA Nanopore sequencing for RNA of dissected endosperm and embryo part of seed at 15 dpa
The reverse transcription required 2 g of total RNA with double-stranded com-plementary DNA (ds-cDNA) and was performed using poly-A specific primers and the MINT cDNA kit (Evrogen, Moscow, Russia), according to the manufacturers instructions. For Nanopore sequencing, a library was prepared from ds-cDNA using the nanopore native barcoding genomic DNA SQK-NBD110-24 (Oxford Nanopore Technologies, Ox-ford, UK), with some modification in the process of using the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing (New England Biolabs, MA, USA).