GSM8228456: LuCaP145.2, JQ1; Homo sapiens; ATAC-seq
Briefly, 50,000 cells per condition were washed in cold PBS and resuspended in 50 μl of cold lysis buffer. Samples were centrifuged for 10 min at 500g, 4 °C and the cell pellet resuspended in the transposition reaction mix and incubated at 37 °C for 30 min. Samples were purified using the Qiagen MinElute PCR Purification Kit. Transposed DNA was eluted in 10 μl of elution buffer,and subjected to PCR amplification using barcoded primers and NEBNext High Fidelity PCR Master Mix. ATAC-seq libraries were purified using 2× volumes of AMPure XP beads to remove fragments below 100 bp. Library quality was assessed using a Bioanalyser High Sensitivity DNA Analysis Kit