Metagenomes of Pisum sativum phyllosphere
Whole leaves were collected in field and stored in sterile 10mM MgCl2 for transport to lab for processing. Leaves were sonicated in fresh buffer for 10 minutes then vortex to dislodge epiphytic bacteria into solution. Leaf wash was passed through a 5um filter to remove plant cells prior to DNA extraction with Qiagen DNAeasy Blood and Tissue kit. Extraction was performed to kit standards with an additional bead beating with 0.4mm silicon microbears at 4 M/S for 15 seconds following the addition of lysis buffer. Metagenomic sequencing was performed at the Cornell Biotechnology Resource Center with Nextera WGS library preparation and Illumina NextSeq500 paired-end 2 x 150bp reads.