Genome sequence of Dematophora necatrix
Genomic DNA was extracted from a single hyphal culture of D. necatrix CMW50482 using the salt-based DNA extraction protocol and sent to Macrogen (Seoul, Korea) where a TruSeq PCR free library was constructed and sequenced on the HiSeq 2500 platform to generate paired-end reads of 251 bp. The sequence data were quality filtered and assembled in CLC Genomics Workbench v.22.0.1 (QIAGEN, Aarhus, Denmark). Quality filter parameters included a minimum quality limit of 0.02, removal of ambiguous nucleotides, removal of homopolymers at the 5 and 3 end when 9 out of 10 in a window are homopolymers and final reads had to be a minimum of 100 nucleotides.