WGS of Bacillus paralicheniformis
A 0.2 ng L1  was used for Library preparation tagmentation, library amplification and clean-up using with the Nextera XT DNA sample preparation kit (Illumina, CA, USA). Subsequent quantification of the library was done using the Qubit dsDNA HS assay on the Qubit 2.0 fluorometer. Library size distribution and quality were assessed with the 2200 TapeStation (Agilent Technologies, CA, USA). A manual library normalisation was performed and pooling of the libraries was done with 5 L of each 2.0 nm normalised library. Subsequently, 600 L of a 12 pm library was made with a 1% PhiX control spike-in. Sequencing was done on an Illumina MiSeq with the v3 chemistry using 2  300 bp paired-end reads.