Metagenomics of a spore former enriched mouse microbial community: Fecal contents extracted with PowerFecal Pro
Libraries were constructed using high molecular weight DNA (approximately 100 ng) using the Nextera DNA Flex Library Prep kit (cat#20025520) with an average insert size of 450 bp.  PCR-amplified libraries were qualified on an Agilent Technologies 2200 TapeStation using a D1000 ScreenTape assay (cat# 5067-5582 and 5067-5583) and the molarity of adapter-modified molecules was defined by quantitative PCR using the Kapa Biosystems Kapa Library Quant Kit (cat#KK4824).  Libraries were normalized and pooled in preparation for Illumina sequencing