GSM7899510: 2fTGH,interferon alpha,24h,phospho-STAT2; Homo sapiens; ChIP-Seq
Cells were cross-linked with DSG for 30 min (or 45min for Huh STAT1KO) and then with formaldehyde for 10 min. After fixation chromatin was sonicated with a Diagenode Bioruptor Plus to generate 200–1000 bp fragments ChIP-Seq libraries were performed using TruSeq ChIP Library Preparation Kit or NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina. 5-10ng of DNA was used as starting material. Libraries were amplified on the thermocycler. Post amplification libraries were size selected at 200-800bp using gel extraction (TruSeq) or 200bp using Agencourt AMPure XP beads (NEBNext). Libraries were then quantified using Qubit dsDNA HS assay kit and quality was assessed by the Agilent 2100 Bioanalyzer (High Sensitivity DNA Kit).