GSM6820672: Breg-1; Homo sapiens; RNA-Seq
1 × 10^6 naïve B cells and Bregs were isolated by FACS Aria II from MPE. A total amount of 3 μg RNA per sample was purified from total RNA using poly-T oligo-attached magnetic beads. Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers, and Index (X) Primer. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumia) according to the manufacturer's instructions.