GSM7900489: RILseq_hfq-3XFLAG minus DPO replicate ii; Vibrio cholerae; OTHER
first strand cDNA synthesis using SuperScript III and Primer AR2, RNA degradation, cDNA cleanup, ligation of second adaptor(3Tr3) to cDNA 3' ends, cDNA cleanup and PCR enrichment using P5_Enr primer and P7_BC1_Enr primer RIL-seq (doi: 10.1038/nprot.2017.115)