GSM7092066: LepRneg_Bone_rep9; Homo sapiens; RNA-Seq
Bone or fibrous tissue obtained from patients undergoing primary joint arthropalsty or revision arthropalsty due to aseptic loosening, respectively, were subjected to both mechnical and enzymatic digestion. Single cells were otbtained, stained for Flow cytometry antibodies and FACS was conducted to sort the desired cell types. RNA sample were prepared using the RNAeasy Plust Micro Kit Cat no 74034. cDNA libraries were generated using the Illumina TruSeq RNA Sample Preparation kit and sequenced on NovaSeq 6000 sequencer