GSM8086911: Nexus-Pol2-Myb-TAD CDK8i; Homo sapiens; ChIP-Seq
Lysate were isolated from formaldehybe cross-linked cells and sonicated to release the chromatin, then immunoprecipitated with antibody. Briefly, the immunoprecipitation and washes were performed using the same conditions as the ChIP protocol. Upon completion of these steps, the DNA was end-repaired, A-tailed, adaptors ligated, exonuclease treated, circularized on Dynabeads as described previously. Constructed via PCR during ChIP-nexus protocol