GSM7400659: 4C-seq, rs79423520 located CRE; Homo sapiens; OTHER
The 4C assay was carried out in diploid HAP1 cells, using a published protocol. Viewpoint-specific amplification was performed with 8 × 200 ng 4C template using 2 × Phanta Max Master Mix. The next-generation library was constructed using the VAHTS Universal DNA Library Prep Kit for Illumina V3 and purified by AMPure XP beads following the manufacturer's instructions. The 4C-seq library were sequenced on the Illumina NovaSeq 6000 platform, producing pair-end reads of 150 bp.