GSM8182636: DMD1_Replicate 2; Homo sapiens; RNA-Seq
Total RNA was isolated from in vitro terminally differentiated myotubes from  WT, patient derived DMD iPSCs (DMD1 and DMD2) and their CRISPR corrected counterparts (DMD1-Cln25 and DMD2-Cln7).TRIzol (Invitrogen) and the PureLink RNA mini kit (Invitrogen) with on-column DNaseI treatment following the manufacturer's recommendations. 500 ng of total RNA was used to for generate unique dual-indexed (UDI) TruSeq stranded mRNA libraries.