GSM7091594: IL21 CBNK day 7  124610 (baseline) ATAC; Homo sapiens; ATAC-seq
Single‐nucleus libraries were generated using the 10X Genomics Chromium Next GEM ATAC Capture and Library (V1.1), following the manufacturer's protocol. Nine samples were pooled to give a final concentration of 10 nM. Pooled samples underwent further qPCR prior to sequencing with the NovaSeq 6000 sequencer using the S1, 100 cycles flow cell and sequenced with read 1 for 50 cycles, i7 8 cycles, i5 index 16 cycles, and read 2 for 49 cycles. By applying RNA  and ATAC sequencing, we want to investigate the epigenetic factors that contributing for IL-21-induced memory formation in NK cells