GSM8275175: OV90CisR1_1; Homo sapiens; RNA-Seq
Total RNA was isolated using Trizol reagent (Invitrogen). RNA quality was assessed by Agilent 2100 bioanalyzer using the RNA 6000 Nano Chip (Agilent Technologies, Amstelveen, The Netherlands), and RNA quantification was performed using ND-2000 Spectrophotometer (Thermo Inc., DE, USA). For control and test RNAs, the construction of library was performed using QuantSeq 3' mRNASeq Library Prep Kit (Lexogen, Inc., Austria) according to the manufacturer's instructions. High-throughput sequencing was performed as single-end 75 sequencing using NextSeq 550 (Illumina, Inc., USA).