GSM8087537: cow.bulk.esc.3; Bos taurus; RNA-Seq
For bulk RNA-seq, total RNA was extracted separately from four bEpiSCs samples using the RNeasy Mini Kit (Qiagen, 74106). For bulk RNA-seq, for constructing strand-specific RNA-seq libraries, we employed a rRNA depletion protocol (Globin-Zero Gold rRNA Removal Kit, Illumina, GZG1224) in conjunction with the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, E7420S). All libraries were quantified using the Qubit dsDNA High Sensitivity Assay Kit (Invitrogen, Life Technologies, Q32851) and sequenced on an Illumina HiSeq 4000 platform.