GSM8023103: CF_P56_Cre+_rep1 [ATAC-Seq]; Mus musculus; ATAC-seq
Cardiac fibroblasts (75000 sorted cells) were isolated using collagenase enzymatic reaction and FACS sorted based on the GFP fluorescence from  Col1a1-GFP allele and on the tdTomato fluorescence driven by the LepR-Cre. Purified Cardiac fibroblasts  were collected in Storing Buffer (10mM HEPES, 2mM MgCl2, 25mM KCl, 250mM Sucrose, 70% Glycerol) and stored at -80C. Tagmentation, PCR-amplification and Libraries  were generated using the ATAC-seq kit (Active Motif# 53150) according to the manufacturer's protocol and sequenced on Nextseq 550 System (Illumina)