GSM7401139: control_MI_rep1; Mus musculus; RNA-Seq
A conditional HIF2αfl/fl mice in the C57BL/6 background were purchased from Jackson laboratory. We bred Hif2αfl/fl mice to the transgenic mice expressing tamoxifen‐inducible Cre recombinase (CreERT2) under the control of the VE-cadherin promoter. To obtain experimental ecHif2α-/-( HIF2αfl/fx+Cre) and controls (Hif2α+l/- and Hif2α+/+Cre) mice were grouped and fed with or without tamoxifen containing food for two weeks. After determining the genotypes, We performed myocardial infarction (MI) in controls and ecHif2a-/- mice.  Heart tissues were harvested 28 days after MI Total RNA from controls and Hif2α-/- heart tissues was isolated 28 days after MI using RNeasy Fibrous Tissue Mini Kit (74704, Qiagen, MD) according to the manufacturer's protocol. RNA integrity was confirmed using a Cytation3 microplate reader (BioTek, VT). Library was performed according to the manufacter's instructions  using Illumina TruSeq Small RNA Sample Preparation Kit (RS-930-1012, Illumina, CA).