GSM6454625: PHH, +HCV JFH-1, Day2, B; Homo sapiens; RNA-Seq
Total RNA from cells were isolated using the RNeasy Mini Kit with DNase treatment (Qiagen, 74106). RNA was quantified using Qubit (Thermo Fischer Scientific) and RNA integrity (RIN) was analyzed using BioAnalyzer 2100 lab-on-chip (Agilent) for a RIN score >7.0. 1.3 ug of total RNA was used for the construction of sequencing libraries. Stranded total-RNA RNA-Seq libraries were synthesized using Illumina TruSeq Total Stranded with RiboZero Gold ribosomal RNA depletion, following manufacturer's protocols. Resulting libraries were quantified and balanced using Qubit (Thermo Fischer Scientific), BioAnalyzer HS lab-on-chip (Agilent), and qPCR (Roche/Kapa).