GSM7663427: er_abm_hsc2; Mus musculus; RNA-Seq
Cells were harvested, lysed, and RNA extraction was performed using the Norgen single cell RNA purification kit (51800, Norgen Biotech). Library preparation was performed using SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian (634418, TaKaRa). Library concentration was measured using QuantIT 1X dsDNA HS Assay Kit (Q33232, Thermo Fisher), and quality was assessed on 5200 Fragment Analyzer™ 12-capillary (M3510AA, Agilent) using DNF-930 dsDNA Reagent Kit, 75 bp – 20,000 bp (K0500, Agilent). The library pool was diluted to a final concentration of 1.25 nM and sequenced on a NovaSeq 6000 System (20012850, Illumina) using NovaSeq 6000 S2 Reagent Kit (2x100 paired end cycles) (20012861, Illumina).