single-cell RNA-sequencing of tumors from PyMT;Shcbp1-/-
For RNA-sequencing (RNA-seq), RNA was extracted from the tumors of PyMT;Shcbp1+/+ and PyMT;Shcbp1-/-mice. RNA quantity and integrity were assessed using a NanoPhotometer spectrophotometer (IMPLEN, CA, USA) and RNA Nano 6000 Assay Kit for the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Sequencing libraries were prepared using the NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA, #E7770) and library quality was assessed using the Agilent Bioanalyzer 2100 system (Agilent Technologies, USA).