GSM8307338: SA-21-input; Mus musculus; RIP-Seq
Total RNA was purified using the RNeasy Mini Kit (QIAGEN) The fragment distribution and concentration of RNAs（after immunoprecipitation） were detected using an Agilent 2100 bioanalyzer (Agilent) and simpliNano spectrophotometer (GE Healthcare).Then, immunoprecipitated RNA or Input was used for library construction with NEBNext® Ultra™II RNA Library Prep Kit for Illumina®(New England Biolabs, E7770). Library quality was assessed on the Agilent Bioanalyzer 2100 system. The libraries were sequenced on an Illumina Novaseq platform with a paired-end read length of 150 bp according to the standard protocols.