P4/Adropin
PCSS were cultured with pre-warmed Minium Essential Media (MEM, #51200-087, Life Technologies), containing 11.34 mM Glucose (#182811, Komptur), 26 mM NaHCO3 (#S8761, Sigma), 25 mM HEPES (#15630, Gibco), 1 mM sodium pyruvate (#11360, Gibico), 2 mM Glutamine GlutaTM (#35050, GIBCO), Penicillin-Streptomycin (#P4333, Sigma) and Amphotericin (#P4333, Sigma) at 37 C, 5 % CO2 and atmospheric O2