RNA-seq of mus musculus: APCs pooled from multiple mice
Library was prepared according to manufacturer's instructions (single cell 3' V3.1 protocol, 10x Genomics). Briefly, samples were resuspended in the master mix and loaded together with partitioning with oil and gel beads into the chip to generate the gel bead-in-emulsion (GEM). The poly-A RNA from cell lysate contained in every single GEM was retrotranscripted to cDNA, which contains a Illumina R1 primer sequence, Unique Molecular Identifier (UMI) and the 10X barcode.  Pooled barcoded cDNA was cleaned up with Silane DynaBeads, amplified by PCR and appropriately sized fragment swere selected for library construction. During library construction, P5, P7, i7 and i5 sample indexes, and TruSeq Read 2 (read 2 primer sequence) are added via End Repair, A-tailing, Adaptor Ligation, and PCR. The final libraries contain the P5 and P7 primers used in Illumina amplification.