GSM6909035: a cell [10-1_S54]; Lachancea kluyveri; RNA-Seq
RNA was extracted using the RiboPure RNA purification kit (ThermoFisher AM1924) according to manufacturer's instructions. Total RNA quality was verified on the Agilent Tapestation. Total RNA was poly-a selected with the NEBNext Poly(A) mRNA Magnetic Isolation Module(Neb E7490S). cDNA synthesis and library preparation was done with the NEBNext Ultra II Directional RNA Library Prep kit for Illumina (Neb E7760L). Quality and concentration of libraries were determined with the Agilent Tapestation. Libraries were pooled in equimolar amounts and sequenced using single end 65 base pair reads on an Illumina HiSeq 4000 in the UCSF Center for Advanced Technologies