GSM7400882: 20220401_Stimulated PBMCs, lane 3, mRNA-derived cDNA; Homo sapiens; RNA-Seq
Cells were processed for expanded cellular indexing of transcriptomes and epitopes by sequencing (exCITE-seq) using Chromium Next GEM Single Cell 5' HT Kit v2. Cells were pooled and loaded onto Chromium HT Chips and run on a Chromium controller (10X Genomics). Gene expression, V(D)J, and surface protein expression libraries were made using the 5' Feature Barcode Kit, Chromium Single Cell V(D)J Amplification Kit, and Chromium Next GEM Single Cell 5' Library Kit (10X Genomics) as recommended by the manufacturer. exCITE-seq