GSM6824854: 293T, m6A-IP, rep2; Homo sapiens; RNA-Seq
The chromosome fractions of parental and METTL3-/- HEK293T cells were extracted and chromosome-associated RNA (caRNA) was isolated with TRIZOL reagent. The resulting caRNA was treated with Turbo DNA-free Kit (Invitrogen) and RiboMinu Human/Mouse Transcriptome Isolation Kit (Thermo Scientific), successively, to remove residual genomic DNA and rRNA by following the manufacturer's protocols. m6A immunoprecipitation was performed with EpiMark® N6-Methyladenosine Enrichment Kit (NEB). m6A-seq Library preparation was performed by using SMARTer Stranded Total RNA-Seq Kit v2 (Takara) according to the manufacturer's protocol