GSM6594825: ENNB_Lo_Liver5; Salmo salar; RNA-Seq
Total RNA was extracted from the distal intestine and liver tissues sampled using QIAzol® Lysis Reagent and the EZ1® RNA Tissue Mini Kit (Qiagen, Crawley, UK) according to the manufacturer's protocol. Total RNA samples were sent to Novogene Europe, Cambridge, UK, for cDNA library preparation and sequencing using the Illumina NovaSeq 6000 platform for 150 bp paired end reads. Enriched mRNA was prepared using Oligo(dT beads). cDNA libraries were prepared following manufacturer's instructions per the Novogen pipeline (Novogene Europe, Cambridge, UK)