RNA-seq of group B Streptococcus A909: Control outgrowth
Illumina TruSeq total RNA kit was used for preparation of the sequencing library, and the Ribo-Zero Plus rRNA Depletion Illumina kit was used for rRNA depletion. Random primers were used to initiate first and second strand cDNA synthesis. The 3 ends were adenylated then adapters ligated and the sequencing library was then amplified with indexing.