GSM7566976: GUIDE seq AsB2M; Homo sapiens; OTHER
Cells were collected after 48h post-transfection for genomic DNA extraction using FastPure Cell/Tissue DNA Isolation Mini Kit(Vazyme) Libraries were prepared according to GUIDE-seq methods. Briefly, Genomic DNA was sheared to length of about 250 bp, added Y adapter, and amplified by two rounds of nested anchored PCR. The last PCR products were GUIDE-seq libraries and sent to the company for sequencing using an Illumina sequencer