Lactobacillus reuteri transcriptome without glycerol in culture medium
Total bacterial RNA was extracted using TRIzol reagent (Life Technologies, Grand Island, NY, USA) and purified using an RNeasy Micro Kit (QIAGEN, Germany). First- and second-strand cDNA were synthesized using a superscript double-stranded cDNA synthesis kit (Life Technologies, USA). inally, the cDNAs were sequenced on an Illumina HiSeq 2500 platform.