GSM7401924: B16, Unsorted, replicate 2, IgG; Mus musculus; ChIP-Seq
B16 F10 cells were trypsinized at day 5, spun down at 400g, then washed and resuspended in 1x DPBS. Low and high pigmented cells were sorted using the side scatter intensity of a flow cytometer. Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a bath sonicator (Diagenode Bioruptor). ChIP-seq libraries were prepared using the NEB Ultra II DNA library prep kit (E7103S) complemented with NEBNext Multiplex Oligos for Illumina (E6440S). Equal ammount of DNA was used as starting material for all samples in each replicate set. Libraries were amplified using 5-7 cycles on the thermocycler. Post amplification cleanup was performed using NEBNext beads. Libraries were validated using the Agilent High Sensitivity DNA Kit.