GSM7665016: Tumor #2 Tom- cells; Mus musculus; RNA-Seq
B220+IgM+Tom+ (Tom+) and B220+IgM+Tom- (Tom-) spleen cells were purified using Sy3200 Cell Sorter (SonyBiotechnology). Total RNA was extracted using PicoPureTM RNA Isolation Kit (ThermoFisher Scientific) Barcoded RNA-seq libraries using the NEBNext RNA Library preparation kit (Illumina). The size of the libraries was checked using the Agilent 2100 Bioanalyzer DNA 1000 chip.