GSM6690585: day8-after-differentiation-stimuli-rep1 (RNA-seq); Mus musculus; RNA-Seq
RNA was extracted according to the standard protocol using TRIzol (Invitrogen). The concentration of extracted total RNA and the RNA integrity number (RIN) were measured using the 4200 TapeStation system (Agilent Technologies). mRNA was enriched from total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB# E7490). RNA-seq library preparation was performed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB# E7770) and fragment sizes of the obtained library were measured using a TapeStation.