GSM6594845: s_GV_D6Ertd527e_KO_r2.SE; Mus musculus; RNA-Seq
Total RNA was extracted from 25 wild-type or D6Ertd527e1–/– fully-grown oocytes from 11-16 weeks old animals using PicoPure RNA Isolation Kit with on-column genomic DNA digestion according to the manufacturer's instruction (Qiagen). RNA-Seq libraries were constructed using the Ovation RNA-Seq system V2 (NuGEN) followed by Ovation Ultralow Library system (DR Multiplex System, NuGEN)