GSM7766030: Sample 6, CTCF, ChIP; Homo sapiens; ChIP-Seq
B cells (~2x106 each sample) were fixed in 1% formaldehyde at room temperature for 10 min. After quenching and cell lysis, chromatin was fragmented with the Covaris E220 system DNA was purified by Zymo DNA Clean & Concentrator kit (Zymo, D4003). Streptavidin C1 beads were used to capture biotin-labeled DNA fragments. The sequencing libraries were generated on the streptavidin C1 beads using Illumina Tagment DNA Enzyme and Buffer kit (Illumina, 20034198)