GSM6937771: NC4; Homo sapiens; Severe acute respiratory syndrome coronavirus 2; OTHER
Spatial transcriptomics. Human placentae from distinct regions including the chorionic villi, decidua, and chorioamniotic membranes, or cross-sections from the parenchyma, were fresh-frozen in optimal cutting temperature solution (FF-OCT). Blocks were cryosectioned and H&E stained directly on 10X Genomics Visium Gene Expression slides (v2) and imaged using a Nikon Eclipse SE Ni microscope using a Nikon DS-Ri1 camera, Nikon Plan Apo objective at 10x magnification (0.45 aperture, 0.91 μm/pixel resolution). Tissues were permeabilized and RNA was subject to spatial transcriptomics library preparation including poly(dT) reverse transcription. Libraries were sequenced on the Illumina NovaSeq S4 platform with 2% PhiX.