RNA sequencing of 10 female pupal stage Diachasmimorpha longicaudata PBARC strain
Total RNA extraction was performed from 10 pooled individuals using the Zymo Direct-zol-96 MagBead RNA kit. 10 ug RNA was then cleaned using the Zymo RNA Clean & Concentrator-5 kit. Cleaned RNA was converted to cDNA with poly(A) (oligodT) priming and prepared for sequencing using the NEBNext Ultra II Directional RNA Library Prep Kit. Sequencing was performed on an Element Aviti platform to yield paired end 150bp reads.