GSM7766031: Spleen 1M P14s Sample 1; Mus musculus; RNA-Seq
Total RNA was extracted from cells following the Trizol Reagent User Guide (Thermo Fisher Scientific). Extracted RNA samples were quantified using Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA). Extracted RNA samples were quantified using Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA) and RNA integrity was checked using Agilent TapeStation 4200 (Agilent Technologies, Palo Alto, CA, USA). RNA libraries for ultra-low RNA-seq were prepared using the SMARTSeq HR Ultra Low Input Kit for full-length cDNA synthesis and amplification (Clontech, Mountain View, CA. Illumina Nextera XT library was used for sequencing library preparation.