GSM7898915: kura_T10; Homo sapiens; OTHER
Cells were trypsinized and single-cell suspensions were counted. Genomic DNA extraction for whole exome sequencing was performed using Qiagen Blood and Cell DNA kit (Catalog no: 13323). The extracted DNA from representative samples (C, T10, T40 and T320) was sent to the Broad Institute facility and processed according to the in-house pipeline. Briefly, 125ng in 50μL was prepared for sequencing using the KAPA Hyper Prep Kit. Hybridization and capture were performed using the relevant components of IDT's XGen hybridization and wash kit and following the manufacturer's suggested protocol. Sequencing was performed using Illumina Novaseq.