GSM8229852: Spleen_NoTreg_1; Mus musculus; RNA-Seq
RNA was isolated using RNeasy Mini kit (Qiagen).RNA concentration and purity were determined using the Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity with a Bioanalyser 2100 (Agilent). 3′mRNA-seq library preparation and transcriptome analysis was performed by Lexogen (Austria) using the QuantSeq 3′mRNA-Seq Library Prep Kit for Illumina and QuantSeq data analysis workflow.