GSM7574577: B16_TdgKO2 stranded RNA-Seq; Mus musculus; RNA-Seq
Total RNA for each sample was extracted using TRIzoI (Thermo Fisher Scientific) according to the manufacturer's instruction. For library preparation, the purified total RNA from KP or B16 cells was quantified by Qubit (Invitrogen) and analyzed by an Agilent Bioanalyzer to assess RNA integrity. 1 μg RNA was used to generate rRNA-depleted RNA with a rRNA Depletion Kit (NEB, E7400) according to the manufacturer's instructions. The rRNA-depleted RNA was analyzed in an Agilent Bioanalyzer to ensure the complete removal of rRNA, and then used to generate directional RNA library with NEBNextUltra™ DirectionalRNA Library Prep Kit for Illumina (NEB, E7760) according to the manufacturer's instructions.