GSM7092533: Th17_MSCV_ADAR2_WT rep1; Mus musculus; RNA-Seq
RNA were isolated using Qiagen Rneasy mini kit (74106) Ribosome-depleted RNAs were used to generate the cDNA libraries using the KAPA Hyper Prep Kit (KR0961) according to the manufacturer's instructions. Briefly, DNA was end-repaired and A-tailed to yield a protruding 3- 'A' base for ligation of Illumina's adapters which have a single 'T' base overhang at the 3' end. Post-ligation clean was used to remove the extra adapters. Then the adapter-ligated DNA was PCR amplified with Illumina primers for 8 cycles, followed with post-amplification clean with KAPA pure beads. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Illumina NovaSeq S4 following the manufacturer's protocols.