GSM7091325: Patient5, non-GVHD, scRNAseq; Homo sapiens; RNA-Seq
All patients enrolled were adult recipients who underwent haploidentical HSCT for acute leukemia and achieved stable engraftment without other severe transplant-related complications including infections, hemorrhagic cystitis. Cyclosporine A, mycophenolate mofetil, and short-term methotrexate were used for GVHD prophylaxis for all recipients. Uniform strategies for infection prevention and supportive care were given. Freshly collected  blood samples were placed into tubes containing EDTA (ethylenediamine tetraacetic acid) and then transferred for single-cell preparations within 10 minutes. Library was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics).