16S rRNA seq of cucumber rhizosphere soil
Amplicons spanning the V3 and V4 regions of the bacterial 16S rRNA gene was sequenced. The primer pair 338F (5'-ACTCCTACGGGAGGCAGCAG-3') /806R (5'-GGACTACHVGGGTWTCTAAT-3') was used to obtain amplicons. PE 2X300 library was established according to the standard procedure of Illumina MiSeq platform.