GSM7568010: Chronic Dop 24h H9 R1; Homo sapiens; RNA-Seq
For RNA extraction, total RNA was extracted as previously described [109]. In brief, hMSN-like cultures were washed 1 time in PBS. Total RNA was isolated using Direct-zol RNA MicroPrep Kit (Zymo Research) according to the manufacturer's protocol. RNA samples were collected in 2 mL RNAse-free tubes and chilled on ice throughout the procedure. Total RNA was then used for RNA-seq. RNA libraries for RNA-seq were prepared by Genewiz/Azenta.