Cc-KHV-leukocytes-10dpc-control3
Common carp (Cyprinus carpio) were exposed to Koi herpesvirus (KHV) and blood samples collected ten days post-challence. Leukocytes were isolated and total RNA was extracted from both control and infected fish samples. Sequencing libraries were prepared with rRNA depletion as part of the lllumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus protocol (Illumina, UK). Samples were pooled in equimolar concentrations before denaturing, diluting, and 150 paired-end (PE) sequencing on an Illumina NovaSeq 6000 platform over 0.4 lane of an SP flowcell (Illumina, UK).