Long-read whole genome Nanopore MinION R10 flowcell sequencing of Chamaecrista mimosoides sample CHA6 using SQK-Q20EA library preparation
High molecular weight genomic DNA was extracted from Chamaecrista sample CHA6 using a standard cetyltrimethylammonium bromide (CTAB) protocol with 1% polyvinylpyrrolidone (PVP). Nanodrop quantification indicated a DNA concentration of 786 ng/l. The sample was diluted 15:50 in nuclease-free water and quantified by Qubit at 181 ng/l. To generate sufficient high-quality DNA, 22 l of the diluted sample (1038 ng total) was purified using a Short Read Eliminator (SRE) kit to remove fragments below 25 kb. After purification, 62.8 ng/l DNA was recovered in 65 l total volume.  Oxford Nanopore library was prepared from 47 l of purified DNA (2951 ng) (diluted further to 49 l) in nuclease-free water using a Q20+ Early Access Kit (SQK-Q20EA) and sequenced on a FLO-MIN112 R10 flowcell. Basecalling was performed using Guppy version 6.0.1.