GSM6909023: Mdivi_L2C_PolII rep1; Mus musculus; ChIP-Seq
Superovulation was performed in 6-to-8-week-old female mice by injection of 10 IU of PMSG, followed by 10 IU of hCG 48 hours later. The ovulated female mice were mated with stud males and embryos were collected from the oviduct ampulla. Cumulus cells were digested by hyaluronidase, and embryos were washed with M2 medium and cultured in KSOM medium. ChIP-seq was performed using a Hyperactive In-Situ ChIP Library Prep Kit for Illumina (pG-Tn5) according to the manufacturer's procedure