Metabarcoding of prokaryotes in marine sediments
DNA extraction with ZymoBIOMICS DNA Miniprep Kit, amplification of the V3-V5 hypervariable regions of the 16S gene done using the primer pairs 357F_ILMN (5- CCTACGGGAGGCAGCAG-3) and 926R_ILMN (5-CCGYCAATTYMTTTRAGTTT-3)