GSM6821201: BS16007; Homo sapiens; RNA-Seq
Cells were washed and suspended in PBS containing 0.04% BSA and immediately processed as follows. Cell viability was assessed on a Countess II automated cell counter (ThermoFisher), and up to 12,000 cells were loaded onto one lane of a 10X Chromium Controller. Single cell capture, barcoding and library preparation were performed using the 10X Chromium platform79 v2 chemistry and according to the manufacturer's protocol (#CG00103) with modifications for generating the hashtag library80. cDNA and libraries were checked for quality on Agilent 4200 Tapestation, quantified by KAPA qPCR, and each library was sequenced independently on a separate lane of an Illumina HiSeq 4000 HO flow cell, targeting 6,000 barcoded cells with an average sequencing depth of 50,000 reads per cell.