GSM6690485: HepG2, CETCh-seq, input; Homo sapiens; ChIP-Seq
Crosslinking: 1% formaldehyde, 10 minutes, room temperature. See Meadows et al. (2020) "Epitope tagging ChIP-seq of DNA binding proteins using CETCh-seq" ChIP-seq libraries were prepared using the ENCODE standardized protocol. 5-50 ng of DNA was used as starting material for input and ip samples. Libraries were amplified using 12 cycles on the thermocycler. Libraries were validated and quantified using KAPA library quantification kit.