18S metabarcoding lab DNA extraction control: lab quality control
An empty 5 mL screw cap tube prefilter extraction control was extracted with PowerSterivex kit reagents with only reagents added. Primers targeting the 18S rRNA gene (Amaral-Zettler et al. 2009) were used to generate amplicons sequenced on an Illumina MiSeq with v3 chemistry generating paired-end reads. Samples were dual indexed with Nextera indices following the Illumina 16S metagenomics protocol (Illumina Part# 15044223 Rev B).