RNA-Seq of brain mestastasis stem cells from colorectal cancers
The RNAs were extracted using the RNeasy Mini Kit (Qiagen) following the manufacturer's protocol and qualified using the Agilent 2100 Bioanalyzer platform (Agilent Technologies). The Sequencing libraries from mRNA were prepared using the TruSeq Stranded mRNA Sample Preparation kit (Illumina) following the manufacturers protocol and paired-end sequenced 150nt on NovaSeq sequencer (Illumina).