GSM7979392: Keap1-null, Nrf2, ChIP, bench 2; Mus musculus; ChIP-Seq
Freshly isolated MuSCs were fixed with 1% formaldehyde for 10 minutes. After quenching, cells were subjected to sonication with Covaris S220. Cell lysates were pre-cleaned by Protein G beads. 1/20 of the lysate was saved as input while the left was incubated with 1.5 μg of Nrf2 antibody.  Libraries were prepared using the NEBNext Ultra II DNA Library Preparation Kit.