GSM6631056: WTcontrol-3; Mus musculus; RNA-Seq
Preparing DNT cells by in vitro conversion of wild type mouse-derived CD4+ cells, then these converted DNT cells (CD3+NK1.1-CD4-CD8-) were sorted using FACS Aria II, and then stimulated with or without UDCA (60uM) for 2 days. And then RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols