RNA-Seq of non-stimulated Vero cells
RNAs were quantified and quality-checked in an Agilent 2100 Bioanalyzer (using the Agilent RNA 6000 Kit). Illuminas TruSeq Stranded mRNA Library Prep Kit was used to prepare the libraries strictly following the manufacturers instructions. Briefly, each sample was enriched in mRNA by selecting those molecules with a poly-A tail at their 3 end. Captured mRNAs are then converted into cDNA, and sequencing adaptors are added to their ends in order to make the samples ready for sequencing. Samples were dual-indexed for post-sequencing demultiplexing. The fragment size distribution and concentration of the libraries were checked in the Agilent 2100 Bioanalyzer (using the Agilent DNA 1000 Kit). The libraries were quantified with the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific). Then, they were pooled in equimolar amounts according to the Qubit results. The resulting pool was sequenced in a fraction of a NovaSeq6000 S4 flow cell (Illumina).