GSM7091173: Parathyroid gland, CKD, ZT14, rep5; Rattus norvegicus; RNA-Seq
For each sample one parathyroid gland was harvested, flash frozen in liquid nitrogen and store at -80 degrees. Total RNA was extracted using TRIzol (Sigma-Aldrich, St Louis, MO, USA) according to the manufacturer's instruction and purified using Qiagen RNase-Free DNase Set (Qiagen, Hilden, Germany) on a QIAcube Connect (Qiagen, Hilden, Germany). RNA quality was examined using an Agilent Technologies 2100 Bioanalyzer. Library preparation was done using a TruSeq Stranded Total RNA Gold kit with dual adaptor indexing strategy; IDT for Illumina – TruSeq RNA UD Indexes (Illumina, San Diego, USA). The library was diluted and pooled to a concentration of 2 nM according to the procedure of “Denature and Dilute Libraries Guide of Illumina Novaseq 6000” and final loading concentration was 400 pM. RNA libraries were prepared for sequencing using standard Illumina protocols