WGBS of human Th17 lymphocytes
Initially, genomic DNA spiked with lambda DNA was fragmented to sizes ranging from 200 to 400 base pairs. These fragments underwent bisulfite treatment to generate single-stranded DNA (ssDNA). Following this, methylation sequencing adapters were ligated, and double-stranded DNA synthesis was carried out to construct the library using the Accel-NGS Methyl-Seq DNA Library Kit for Illumina. Subsequently, size selection and PCR amplification were employed to prepare the library, which was then quantified using Qubit and real-time PCR. The size distribution of the quantified libraries was assessed using a bioanalyzer. Finally, the quantified libraries were pooled and sequenced using Illumina platforms.