GSM7663973: PDO21 and CAF co-culture SPLiT-seq experiment sublibrary 2; Homo sapiens; RNA-Seq
On day 9, organoids were dissociated into single cells using TrypLE and incubated for 10 minutes at 37 °C on a heated orbital shaker at 300 rpm. Sample preparation was then performed based on the SPLiT-seq protocol. Single-cell RNA-seq libraries were prepared using the SPLiT-seq protocol