Sequencing of small RNAs from the ooplasm, replicate 2
Oocyte cytoplasm was collected after nucleus isolation. Total RNA, including small RNA species, was isolated by TRIzol reagent. RNA was isolated according to the manufacturers recommendations, RNA was precipitated in the presence of  GlycoBlue. The RNA pellet was air dried, and dissolved in RNase-free water and stored at -80C.