SWB-S1-2 Illumina PE WGS
Using the unopened floral buds, we used a DNeasy Plant Mini Kit (Qiagen) to extract DNA. DNA was prepared for sequencing using the Illumina TruSeq Nano DNA Library prep kit with IDT for Illumina Unique Dual Index adapters following manufacturer's recommendations. We loaded the libraries onto one lane of an Illumina NovaSeq 6000 S4 flow cell and conducted sequencing in a 2x150 bp paired end format using a NovaSeq v1.5 300 cycle reagent kit. Base calling was done by Illumina Real Time Analysis (RTA) v3.4.4 and output of RTA was demultiplexed and converted to FastQ format with Illumina Bcf2fastq v2.20.0.