GSM8229509: hVICs-OM-2; Homo sapiens; RNA-Seq
Total RNA was isolated from the hVICs treated for 24 hours using Trizol reagent (Invitrogen, Carlsbad, CA). After solubilization, the addition of chloroform induces phase separation, wherein proteins are extracted to the organic phase, DNA settles at the interface, and RNA remains in the aqueous phase. Furthermore, we conducted a quality examination of all extracted RNAs using a NanoDrop spectrophotometer, only high-quality RNA samples (A260/280 ≥ 2.00) were selected for the subsequent experiments Library preparation utilized the VAHTS Universal V6 RNA-seq Library Prep Kit according to the manufacturer's instructions.