GSM8022705: snRNAseq, O_Post-LVAD; Homo sapiens; RNA-Seq
We isolated nuclei from frozen human heart tissue samples and constructed 3' single cell gene expression libraries (Next GEM v3.1) using the 10x Genomics Chromium system. The libraries were sequenced with ~200 million PE150 reads per sample on Illumina NovaSeq. After sequencing, clean reads were analyzed with human reference genome GRCh38 using Cell Ranger v6.1.2. Introns were included in the analysis. single-nuclei RNA-seq library construction following the protocol provided by 10X Genomics.