GSM7664111: IL-38 neutralization - IgG_5; Mus musculus; RNA-Seq
Total RNA from PyMT tumors was isolated using TRIzol Reagent (Life Technologies), followed by RNA purification with RNA Clean & Concentrator-5 (Zymo Research). RNA quality was evaluated in Agilent Tape Station 4150 with RNA Screen Tape (Agilent) and concentration measured by Qubit HS RNA Assay Kit (Thermo Fisher Scientific). Libraries were prepared using QuantSeq 3' mRNA-Seq Library Prep Kit FWD from Illumina (Lexogen)