DRY4: Incorporating seasonally and spatially dynamic ectomycorrhizal fungal communities strengthens predictions of soil biogeochemical cycling in neotropical dry forests
Total genomic DNA was extracted from each sample using a DNEasy PowerSoil Kit (Qiagen, Hilden, Germany) following manufacturers instructions. All extractions were secondarily cleaned using a DNEasy PowerClean Pro Cleanup Kit (Qiagen, Hilden, Germany) following manufacturers instructions. The ITS1 rDNA subunit was PCR amplified using a barcoded fungal-specific ITS1F-ITS2 primer set and cycling conditions detailed in Smith and Peay (2014), with the exception of having 35 rather than 30 total cycles. Both negative and positive (Palmer et al. 2018) controls were included. All samples were cleaned and normalized using the Charm Just-A-Plate kit (Charm, San Diego, CA, USA) following manufacturers instructions. Samples were quantified on a Qubit fluorometer (Thermo Scientific, Waltham, MA, USA), mixed at an equimolar concentration into a single library, and sequenced using Illumina MiSeq 2 x 250 bp v2 chemistry at the University of Minnesota Genomics Center