Illumina NovaSeq 6000 paired end sequencing; RNA-seq of mature and immature human induced pluripotent stem cell-derived brain endothelial cell-like cells
RNA-seq of mature and immature human induced pluripotent stem cell-derived brain endothelial cell-like cells
All samples were collected on the 9th day of differentiation irrespective of treatment. Total RNA was isolated using TRIzol (Sigma) Samples were prepared for sequencing using the TruSeq RNA sample prep kit (Illumina) to prepare cDNA libraries after ribosome depletion.