GSM6594906: CEA, Sample 13; Mus musculus; RNA-Seq
Cells were hand-picked into PCR strip tubes containing 3 μl lysis buffer (0.2% TritonX-100 (Sigma) and 0.1 U/μl RNase inhibitor (Lucigen)) and cDNA libraries were prepared using the Smart-SCRB chemistry as described previously (Cembrowski et al., 2018; Xu et al., 2020). 600 pg cDNA from each pooled sample was used in a modified Nextera XT library preparation (Illumina) (Soumillon et al., 2014) using the P5NEXTPT5 primer and extending the tagmentation time to 15 min. The resulting libraries were purified according to the Nextera XT protocol (0.6x beads ratio) and quantified by qPCR using Kapa Library Quantification (Kapa Biosystems).