GSM7092571: E4_2, egr-2_RNAi, 4_dpa, biol_rep_2; Schmidtea mediterranea; RNA-Seq
Total RNA was extracted from the anterior third of 10 planarian tail fragments per biological replicate using Trizol as described (Forsthoefel et al., eLife, 2020). Total RNA for 4 biological replicates per condition was submitted to GENEWIZ (South Plainfield, NJ) for library generation from ~ 500 ng/sample using the NEB NEXT ULTRA Library Prep Kit and RNA sequencing with standard Illumina adapters.