GSM7663601: Tumor-infiltrating lymphocytes,RNA; Mus musculus; RNA-Seq
Tumor-infiltrating lymphocytes of Mcrs1ox or control tumor were isolated, blocked with TruStain FcX and stained with TCR-b (H57-597) and CD90.2 (30-H12) for 30min at 4°C. Cells were then washed twice and TCRb+ CD90.2+ cells were sorted with a FACSAria Fusion Flow Cytometer, with dead cells excluded as DAPI+. Sorted cells were stained with the Biolegend TotalSeq Hashtags and loaded into a 10x Chromium at a density of 1x 10^3 cells/uL in 0.04% BSA. Library was prepared with a Chromium Single Cell 3' v2 Reagent Kits (10X Genomics) following the manufacurer's instruction.