Illumina NovaSeq 6000 sequencing; Inflammatory signals from fatty bone marrow support DNMT3a-driven clonal hematopoiesis (wt/DNMT3A KI subset)
Inflammatory signals from fatty bone marrow support DNMT3a-driven clonal hematopoiesis (wt/DNMT3A KI subset)
Bone marrow cells were collected from the femora and tibia bones. BM was flushed with a 1cc (23G) into IMDM (Iscove's Modified Dulbecco's Medium). The cells were enriched by a c-kit CD117 and sorted by FACS. RNA extraction was performed according to manufacturer's protocol. Libraries were generated according to Keren-Shaul et al MARS-seq protocol.