GSM7401222: hepatocytes isolated from healthy mice, Day 0 [H2]; Mus musculus; ATAC-seq
5× 10^4 cells were used for every single reaction. Cells were washed in cold PBS and resuspended in 50 μl lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.5% NP-40) for 10 min, and nuclei were spun at 500 g for 10 min using a refrigerated centrifuge ATAC-seq libraries were prepared using Trueprep DNA library Prep Kit V2 for Illumina (vazyme).