GSM7574425: B16_WT ATAC-seq; Mus musculus; ATAC-seq
A total of 5 × 104 B16 cells were collected and washed with ice-cold PBS, followed by suspending in cold lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630) to isolate nucleis. Isolated nuclei were then tagmented with Tn5 transposase and libraries were constructed using TruePrepTM DNA Library Prep Kit V2 for Illumina (Vazyme TD501). Amplified libraries were purified using MinElute columns and quantified with a Bioanalyzer using high-sensitivity DNA chips (Agilent 2100). ATAC-seq libraries were pooled and sequenced using an Illumina NovaSeq 6000 platform with paired-end reads of 150 bases.