GSM6594439: wk08-NS, replicate 1, scRNAseq; Homo sapiens; RNA-Seq
Four individual cortical organoids having similar size were obtained from respective samples (WT, NS, and NS-Cor) at different developmental periods (week 8, 16, and 24) and subjected to the scRNA-seq library preparation. Each cortical organoid was dissected into small pieces using a 29G-syringe needle and dissociated using the papain dissociation system (Worthington Biochemical Corporation, Lakewood, NJ) according to the manufacturer's protocol. Dissociated cells were suspended in 1ml of PBS solution supplemented with 0.04% BSA and 10μM Y-27632, counted with LUNA-II automated cell counter (Logos Biosystems, Anyang, Korea), and diluted at a concentration of 1x106 cells/ml. scRNA-seq library was prepared by using Chromium single cell 3' reagent kits v3.1 chemistry (10x Genomics, Pleasanton, CA).