GSM7663752: 1hr post-wounding, biol. rep 3; Gallus gallus; RNA-Seq
RNA was harvested using  using Rneasy mini kit (Qiagen). Total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared following Novogene's protocols and sequenced on an Illumina Novaseq platform and 150 bp paired-end reads were generated. Libarary preparation was performed by Novogene. mRNA purified from total RNA using poly-T oligo-attached  magnetic beads. PCR products purfied on AMPure XP system and library quality was assessed on the Agilent Bioanalyzer 2100 system. Index-coded samples were clustered on a cBot Cluster Generation System via TruSeq PE Cluster Kit v3-cBot-Hs (Illumia). Library preparations were sequenced on an Illumina Novaseq platform to generate 150bp paired reads.