GSM8229062: Neo_Kras_p53_2; Mus musculus; RNA-Seq
DNA and RNA were extracted from the same tumor tissue piece, approximately 20-30 mg in weight, using Qiagen's DNA/RNA AllPrep kit (80204) following the manufacturer's protocol. Only samples with RNA integrity scores (RIN) exceeding 7 proceeded to library preparation using the TruSeq Stranded total mRNA protocol, starting with 50 μl of undegraded RNA at concentrations ranging from 50 to 80 ng/μl per sample.