GSM7574829: ES2-EC359- 1; Homo sapiens; RNA-Seq
ES2 cells are treated with vehicle (0.1% DMSO) or EC359 (10 nM) for 12 hours and total RNA was isolated using RNeasy mini kit using manufacturer's protocol (Qiagen, Valencia, CA). Total RNA samples about 500ng were used for sequencing library preparation according to Illumina TruSeq Stradned mRNA Sample Preparation Guide (Illumina Cat. #: RS-122-2201). Each library was bar-coded and then pooled for cluster generation and sequencing run with 50bp single-end (SE) sequencing protocol.