GSM7401944: TL_IH_NF; Homo sapiens; OTHER
RNA was harvested with Trizol reagent, followed by chloroform extraction and isopropanol precipitation. For all nucleotide recoding RNA sequencing (NR-seq) methods, genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. RNA for NR-seq was treated with nucleotide conversion chemistry as described in Schofield et al. Nature Methods 2018, Riml et al. Angew Chem Int Ed Engl 2017, or Herzog et al. Nat Methods 2017. NR-seq libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413).