GSM7566701: EGFP(R96H)+Arg-tRNA_His(GUG)_tRNA_rep3; Homo sapiens; OTHER
MSR-seq experiments were performed on 3 independent replicates from HEK293 cells overexpressing the mistranslation reporters with and without cognate mc-tRNAs. HEK293 cells were sorted on the FACSAria Fusion 5-18 cell sorter for mCherry+, i.e., successfully transfected, cell populations 48 h post-transfection. Total RNAs were extracted from sorted cells with TRIzolTM reagent (Invitrogen Cat. 15596026). 1 µg of total RNA per sample was used as input for MSR-seq library construct. Libraries for tRNAs were constructed as previously described for multiplex small RNA-seq (MSR-seq) (PMID: 35513407).