The full-length transcriptome sequencing of a  mix sample on PacBio Sequel ll
Equal quantities of RNA from five tissue samples, i.e. root, young trophophyll, young sporophyll, mature trophophyll, and mature sporophyll, were used for PacBio cDNA library construction. Briefly, a total of 1 g RNA from the mixed RNA sample was reverse transcribed using the Clontech SMARTer polymerase chain reaction (PCR) cDNA Synthesis Kit and oligo (dT) to obtain the first-strand cDNA. The second strand was synthesized and amplified with the 5 PCR Primer to generate barcode full-length cDNA. Size selection was carried out on a BluePippin Size Selection System protocol with two size bins for the mixed sample: 1-4 kb and > 4 kb. An SMRT cell was sequenced on the PacBio Sequal platform.