3 prime RNA-seq of Sus scrofa:male piglet Pituitary
Double stranded DNA (dsDNA) was constructed from the mRNA by random-primed reverse transcription and second-strand cDNA synthesis. Sequence libraries were constructed with the 3' mRNA-Seq library prep kit for Illumina (FWD) from QuantSeq-Lexogen (Catn015.96). Library quality and size range was assessed using a Bioanalyzer (Agilent) with the DNA 1000 kit (Agilent) according to the manufacturer's recommendations