RNA-Seq of Odontomachus chelifer: venom gland
The sample was treated with RNAse-free DNAse I (Invitrogen) in a reaction containing 3 g of RNA to a final volume of 20 L. Poly (A) mRNA was isolated with oligo(dT) beads and transcriptome construction occurred using TruSeq Small RNA Library Preparation Kits (Illumina), according to the manufacturers instructions.