GSM6909142: SMA patient after six months of  Nusinersen treatment  [4_T6]; Homo sapiens; RNA-Seq
RNA was extracted using miRNeasy Serum/Plasma (Qiagen) Libraries were prepared starting from 500 ng of total RNA. RNA was fractioned for rRNA depletion using RiboCop V1.3 (Lexogen) and libraries were prepared with the CORALL Total RNA-Seq Library Prep Kit (Lexogen) following manufacturer's intructions. The quality of libraries was analyzed using 4200 Tape Station with a “DNA High sensitivity” assay (Agilent) and quantified using High Sensitivity dsDNA assay with a Qubit device (Life Technologies). Sequencing was performed using Illumina NextSeq 500.