single cell RNA seq of mouse CCA tumor: SB1 adjacent liver sample 2
The viable CD45 positive cells were sorted using a flow sorter and stained with TotalSeq-C Mouse Universal Cocktail (Biolegend V1.0, 199903). The cells were captured, and the cDNA library was prepared using Chromium Single Cell 5' Reagent Kits to target 10000 cell per sample.