GSM6935642: Emcn+/+_310; Mus musculus; RNA-Seq
Femura, illia and tibiae were isolated from the hind legs and the bones were crushed in a mortar. The bone marrow was re-suspended in PBS/3%FCS and filtered through a 70μm cell strainer (Falcon) to obtain single-cell suspensions.  For cell sorting, bone marrow cells were depleted of lineage positive cells using a Lineage Cell Depletion kit (Miltenyi Biotec, cat-nr.130-090-858) according to manufacturer'