GSM8307185: sp replicate1; Salmonella enterica subsp. enterica serovar Pullorum; ncRNA-Seq
1 µg of total RNA was treated sequentially with phosphatase and PNK kinase (New England Biolabs). The treated RNA was subjected to library construction by GenSeq's Small RNA Library Kit. The QC-controlled libraries were subjected to 150bp bipartite sequencing by Illumina NovaSeq High-Throughput Sequencer. RNA libraries were prepared for sequencing using standard Illumina protocols