GSM6824894: WT GFP+; Mus musculus; RNA-Seq
DGs from 5 WT and 5 KO adult mice were harvested and mechanically dissosciated then resuspended and washed in HBSS before cell sorting for EYFP+ cells for input into 10x genomics chip as per manufacturer instructions. 10X Genomic's RNA-seq library was sequenced using paired-end 150bp approach on an IlluminaNovaSeq 6000sequencer. CellRanger v7.0.1 was used to demultiplex, align, and deduplicate sequencing reads in BCL files. Single-cell data in feature-barcode matrices were then processed using Seurat v4.1.1s default pipeline to identify unsupervised cell clusters and generate a t-Distributed Stochastic Neighbor Embedding (t-SNE) plot. Seurat's feature expression analysis was used to visualize genes markers known to be expressed in cell populations displayed in the t-SNE plot.