GSM8279056: Differentiated PC12 cells expressing AR111Q K387/519R, DHT treatment, rep3; Rattus norvegicus; RNA-Seq
RNA was extracted using TRIzol Reagent (Invitrogen) and purified using Direct-zol RNA Miniprep Kit mRNA enrichment, mRNA fragmentation, and random priming; first and second strand cDNA synthesis; end repair, 5' Phosphorylation, and da-Tailing; adaptor ligation, PCR enrichment, and sequencing via polyA selection