GSM6374047: P6MC_SPD-3; Homo sapiens; RNA-Seq
Total RNAs were obtained from human primary melanocytes using Trizol reagent (Invitrogen Life Technologies, Grand Island, NY). Poly(A) mRNA isolation from the total RNA and subsequent fragmentation were performed using Illumina TruSeq Stranded mRNA LT Sample Prep Kit, according to the manufacturer's instructions. RNA libraries were prepared for sequencing using standard Illumina protocols