DNAseq of digestate
The diversity of the microbiome was analyzed by high-throughput sequencing of the hypervariable region V3-V4 of 16S rDNA gene. Specific 341F and 785R primer sequences were used to amplify the selected region and prepare the library. The next-generation sequencing was performed by Genomed S.A. (Poland) with the use of the MiSeq platform (2  300 bp). The PCR assay was conducted with the use of the Q5 Hot Start High-Fidelity 2X Master Mix, according to the manufacturer's protocol. The sequencing analysis was performed in the MiSeq system (2 x 300 bp) in paired-end (PE) technology using the v3 kit for Illumina. A preliminary sequence analysis was conducted in the MiSeq sequencer with MiSeq Reporter (MSR) software.