GSM7663955: Donor 13, PAK1; Homo sapiens; RNA-Seq
RNA extraction was performed using QIAGEN RNase kit. 250ng of total RNA from each samples was used for sequencing library preperation with KAPA mRNA Hyperp Kit according to user manual. The library QC was checked with Agilent bioanalyzer DNA high sensitivity chip, and qubit was used for library quantification. The final libries were sequenced on Illumina Hiseq2500 wtih single read mode of 51 cyles, plus 7 cycles of index sequencing with V4 kit.