GSM7091072: day 62, 4X; Homo sapiens; RNA-Seq
On 16, 28, and 62 days in vitro (DIV), cultured cells were dissociated into single cells using Accutase. On 16 DIV, neurospheres (n = 10 biological replicates per cell line) were pooled together, on 28 DIV, four biological replicates per cell line were pooled together, and on 62 DIV, four biological replicates per cell line were pooled together. To construct the library, the 10X Genomics Chromium Next GEM Single Cell 3' kit v 3.1 was used according to a standard protocol. Each of the four groups (H9 DIV16, 4X DIV16, H9 DIV28, 4X DIV28, H9 DIV62, and 4X DIV62) was run in separate lanes of the Chromium controller, and a total of 8,000 cells were loaded per lane.