WGS of blood: iPSCORE_92_1
WGS of 4 samples (including this one) were performed at the UCSD Institute for Genomic Medicine (IGM) Genomics Center, while most WGS data were generated at HLI. Genomic DNA was isolated from blood (or in 19 cases directly from the fibroblasts, DNEasy Blood & Tissue Kit), quantified, normalized, and sheared with a Covaris LE220 instrument. The samples were normalized to 1ug and submitted for whole genome sequencing. DNA libraries were prepared (KAPA Hyper Prep, Roche), characterized in regards to size (4200 TapeStation, Agilent Technologies) and concentration (Qubit 2.0, Life Technologies), combined into a single pool, and sequenced on the NovaSeq 6000 (150 base paired-end). In total, germline whole genome sequencing (WGS) was performed for 274 subjects though only 222 were reprogrammed into iPSCs.