GSM7663217: DRIP_CPT60_rep2; Homo sapiens; OTHER
Cells were harvested by using accutase, pelleted, resuspended in 1X TE buffer (Tris HCl pH 7.6 10 mM, EDTA pH 8 1 mM) and lysed O/N at 37°C with 0.5% SDS and 62.5 μg/ml in 1X TE buffer. Chromatin was then precipited with isopropanolo and sodio acetato, washed with ethanol and resuspeded in 1X TE for 4h at 30°C. O/N digestion was performed with restriction enzymes cocktail Library construction using the ThruPLEX DNA-Seq kit.