GSM7716355: G12T; Homo sapiens; RNA-Seq
Tissues were stored in RNAlater and lysed with Trizol. Tissues were homogenized and extracted for total RNA with Direct-Zol Miniprep kit (Zymo) with DNase-treatment. Ribosomal RNA was removed, and sequencing libraries were prepared using NEBnext Ultra Low Input Total RNA with rRNA Depletion Prep (NEB).