cox1 metabarcoding of 10L prefilter filtered shipboard freshwater from Niskin Sedna: freshwater control
10L of water shipboard freshwater added to bleached Niskin Sedna was filtered through a 0.8 micron prefilter. DNA was extracted using the Qiagen PowerSterivex kit. Primers targeting the mitochondrial cox1 gene gene (Leray et al. 2013) were used to generate amplicons sequenced on an Illumina MiSeq with v3 chemistry generating paired-end reads. Samples were dual indexed with Nextera indices following the Illumina 16S metagenomics protocol (Illumina Part# 15044223 Rev B).