GSM6594600: astrocyte tgfb2 , biological replicate # C; Mus musculus; RNA-Seq
We lysed ~200 FACS-sorted cells/sample in a lysis buffer containing dNTP and oligo-dT primers. PolyA+ RNA was converted to cDNA using a reverse transcriptase reaction. For cDNA fragmentation and barcoding, we employed the Tn5-based tagmentation procedure. We used Illumina-compatible barcodes (8 base pairs, dual-index). We lysed ~200 FACS-sorted cells/sample in a lysis buffer containing dNTP and oligo-dT primers. PolyA+ RNA was converted to cDNA using a reverse transcriptase reaction. For cDNA fragmentation and barcoding, we employed the Tn5-based tagmentation procedure. We used Illumina-compatible barcodes (8 base pairs, dual-index).