GSM7898415: experimental, rep3, scRNAseq; Mus musculus; RNA-Seq
Brains of control (tdTOM+, N=3) or experimental (Ascl1-Overexpression; GFP+, N=3) tumor-bearing mice with neurological symptoms were freshly harvested and tumors were dissected out in a bath of Hank's Balanced Salt Solution (without calcium or magnesium). Working on ice, tumor tissue was weighed and then transferred to a gentleMACS C tube and dissociated using the Miltenyi Neural Tissue Dissociation Kit (P) and an Octo-Dissociator with Heaters. Following this, cells underwent FACS The 10x Chromium Next GEM 3' protocol was used to create 3' libraries for sequencing at the University of Colorado Anschutz Medical Campus's Genomics Shared Resource Cancer Center using Illumina NovaSEQ 6000 instruments on S4 flow cells. Briefly, cells were lysed and barcoded within each GEM before first strand cDNA synthesis, and also within each GEM. Cells were pooled prior to library completion as described in the manufacturer's protocol.