GSM7766651: C024-14; Homo sapiens; RNA-Seq
Total RNA of each sample was extracted by using RNeasy Mini Kit (Qiagen, Germany) according to the manufacturer's instructions. The amount of total RNA was measured by Nanodrop 1000 (ThermoFisher Scientific, USA), and the integrity of RNA was checked by 2100 Bioanalyzer System (Agilent, USA). The transcriptome libraries were constructed by using NEBNext® Ultra RNA Library Prep Kit for Illumina® (NEB, USA) according to the manufacturer's recommendations as described previously.