GSM8229786: S03_M2; Homo sapiens; RNA-Seq
To extract RNA, cells were harvested, washed with PBS and FACS sorted to have highly pure macrophage population. Then, cells were lysed in TRIzolTM land RNA extracted using Rneasy® Mini Kit (Qiagen), according to the manufacturers' protocol. Extracted RNA samples were quantified by NanoDrop™ 8000 Spectrophotometer (Thermo Fisher Scientific) and quality tested on TapeStation 4200 (Agilent). For sequencing library preparation, 100ng of total RNA was used as input for a poly-A selection. Libraries were prepared according to NovaSeq 6000 Sequencing System.