Effects of Laccaria bicolor treatment on Populus × canescens leaf transcriptome
Effects of Laccaria bicolor treatment on Populus × canescens leaf transcriptome
Per plant: harvest of full leaf (3,4,5) without petiole, pooling and freezing in liquid nitrogen Populus × canescens plants were in vitro propagated in glass jars (weck jars) according to Müller et al. 2013 Frontiers in Plant Science. Four week old Populus × canescens were co-cultivated with Laccaria bicolor (sandwich culture) according to Müller et al. 2013 Frontiers in Plant Science. The co-cultures were incubated for one, two, four and seven days until sample collection. The experiment was independently repeated in three times. Total RNA was extracted with innuPrep Plant RNA kit (Analytik Jena, Jena, Germany). The RNA of three samples was pooled. The library was constructed according to the manufacturer's protocol