Micobiome of soft-ripened cheese
Amplification was conducted using complete fusion primers that included Illumina Nextera adaptors, indices, and sequences targeting the V4-V5 region of the 16S rRNA gene (515F: 5- GTGYCAGCMGCCGCGGTAA-3 and 906R: 5-CCGYCAATTYMTTTRAGTTT-3; Parada et al., 2016). The pooled library was then subjected to cleanup using the AmpureXP PCR cleanup protocol. Sequencing of the pooled library was conducted using an Illumina MiSeq sequencer with Reagent Kit v3 (600-cycle) following the manufacturers recommendations, and with the addition of 10% PhiX.