JE2 CDM with Pyruvate Replicate 1
Staphylococcus aureus USA300 JE2 WT or transposon mutant were grown to OD of 0.4 in CDM with a given carbon source. The pellets were then harvested for RNAseq. Zymo Research Fungal/Bacterial RNA prep kit was used to isolate and purify the RNA. The cells were lysed using a Roche MagnaLyzer instrument. RNA quality was checked with an Agilent Bioanalyzer instrument and ribosomal RNA was removed using an RiboRid protocol. The final sample enriched for mRNA was used in the first step of the Kapa Biosystems RNAseq library prep kit.