17K_SER_b12_rep4
Cells were treated with BODIPY FL LDL in optiMEM. Cells were trypsinized and sorted into bins. After sorting, genomic DNA was isolated from cells and used to amplify the U6-3 to gRNA hairpin region with different in-line barcodes. PCR2 was performed to add full-length Illumina sequencing adapters using internally ordered primers with equivalent sequences to NEBNext Index Primer Sets 1 and 2 (New England Biolabs).