16S metabarcoding of tomato soils
DNA was extracted from 0.25 g rhizosphere or bulk soil with Qiagen DNeasy PowerSoil HTP 96 kits. Samples were sent to the University of Minnesota Genomics Center for sequencing of the V4 region of the 16S ribosomal gene and the ITS2 region of the ITS nuclear ribosomal gene. Sequencing was performed with V3 chemistry and paired end 300 bp sequencing for both markers. Primers used for 16S were the standard V4 region primers 515F and 806R. Primers used for ITS were the ITS2 region primers 5.8SR and ITS4.