Dry season:Rainforest:Onthophagus diabolicus
Metabarcoding data.The PCR reaction mixture underwent pre-denaturation at 94C for 1 minute for a single cycle. Then the mixture was subjected to DNA denaturation at 94C for 20 seconds, and then the reaction temperature was lowered to 54C for 30 seconds to allow the primers to anneal to the DNA template. Then the reaction temperature was raised to 72C for 30 seconds to facilitate the extension of the primers and synthesis of new DNA strands. The denaturation, annealing, and extension cycle was repeated for 25-30 cycles. After the final denaturation, annealing, and extension cycle, a final PCR step was performed at 72C for 5 minutes in a single cycle to ensure complete DNA synthesis. The reaction mixture was then incubated at 4C to stop further enzymatic activity. For each sample, we performed three technical replicates of the PCR. We mixed equal amounts of PCR products in the linear phase from these replicates for subsequent library construction.