Environmental DNA sequences collected from soil
A total of 34 soil samples were delivered under cold conditions to the Unidad de Genmica y Bioinformtica, BIOREN-Universidad de La Frontera (Temuco, Chile), where DNA extraction and amplicon library preparation were carried out. DNA was extracted from the soil samples (0.25 g) using the PowerSoilDNA Isolation Kit (Mo Bio Laboratories), according to the manufacturer's instructions. DNA samples were then prepared using barcoded library adaptors (400 nM) containing the fungal primers ITS1F (CTTGGTCATTTAGAGGAAGTAA) and ITS2 (GCTGCGTTCTTCATCGATGC).