RAD-seq of Robinia pseudoacacia: mature tree sample
Samples were double digested with PstI and MspI enzymes, double-sided size selected in the range of 300  600 bp, inserts were quantified then ligated to adapters and purified. PCR amplification was carried out and index primers were added, and last quality and quantity of the amplicon library was checked with fluorometer.Pooled libraries were diluted  and sequenced on Illumina MiSeq.