Demultiplexed 18S sequences from seawater sampled in 2018 from Dongsha Atoll (Taiwan) in the South China Sea
Libraries for sequencing were made by pooling amplicons into equimolar ratios based on PCR Ct values and the endpoint of amplification curves, which were then size selected using a Pippin Prep (Sage Science, MA; 100 bp to 600 bp) and purified using the Qiaquick PCR Purification Kit (Qiagen Inc.). The volume of purified library added to the sequencing run was determined against DNA standards of known molarity on a LabChip GX Touch (PerkinElmer Health Sciences, MA). Final libraries were sequenced in a paired end approach using a 500 cycle MiSeq V2 Reagent Kit and standard flow cell on an Illumina MiSeq platform (Illumina, CA) located in the TrEnD Laboratory at Curtin University in Perth, Western Australia.