GSM8228732: Microbiota of oral swab from DM139 mice; Mus musculus; OTHER
Genomic DNA from stool and oral swab was extracted using the DNeasy PowerLyzer PowerSoil Kit (Qiagen, Hilden, Germany), while the DNA from lung and colon fresh tissue with DNeasy Blood and Tissue kit (Qiagen, Hilden, Germany) Illumina 16S Metagenomic Sequencing Library Preparation protocol ( see https://emea.support.illumina.com) Amplicons of the variable V3-V4 region of the bacterial 16S rRNA gene were sequenced in paired-end (2x300)