Raw RNA sequence reads for wood stork genome annotation
Extraction of total RNA was performed using the RNeasy Plus Mini Kit (Qiagen, Valencia, CA, USA) following the manufacturer's protocol. DNase treatment, AMPure bead cleanup (Beckman Coulter Life Sciences, Indianapolis, IN, USA), and Qiagen FastSelect HMR rRNA (Qiagen) depletion were prepared prior to library preparation. Library preparation was performed using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) following the manufacturer's protocol. The library was run on the NovaSeq6000 platform (Illumina, San Diego, CA, USA) in 2 x 150 bp configuration.