mtDNA of the Satanas beetle, Dynastes satanas
This study involved sequencing of genomic DNA extracted from Dynastes satanas. The sequencing libraries were prepared from 1g of DNA, fragmented using Covaris M220 to 300~500bp. End repair, A-tailing, and indexing were performed using the TruSeq Nano DNA Sample Prep Kit. Libraries were enriched with 8 cycles of PCR and gel purified using 2% certified low-range ultra agarose gel. Quantification was done using TBS380 (Picogreen) and libraries were pooled and sequenced on an Illumina NovaSeq platform using paired-end 2x150bp reads.