Hi-C sequencing data of HCT116 cells 4 hours after heat shock, replicate 2
First crosslink chromatin material using formaldehyde. Then digest the chromatin with a restriction enzyme cocktail. The 5'-overhangs are biotinylated, and the spatially close digested ends of DNA are ligated, purified, fragmented and enriched for the biotinylated fragments before being subject to custom library prep.