GSM7662987: PBS, Biol Rep 1; Mus musculus; RNA-Seq
Mice were sacrificed 24 hours the last injection. Hind legs were dissected and bone marrow (BM) cells were harvested by flushing both femur and tibia. Whole bone marrow cells were subjected to RBC lysis and were lineage-depleted using magnetic-activated cell sorting (MACS)-based method with EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (StemCell Technologies). Lineage-depleted BM cells were stained and HSPCs were fluorescence-activated cell sorting (FACS)-sorted using the FACSAria Fusion sorter (BD). RNA were extracted from HSPCs using RNeasy Micro Plus kit according to manufactuer's protocol. Library was constructed using the NEXTFLEX Rapid Directional RNA-Seq Kit v2.0 according to manufacturer's protocol