GSM6820644: E233S_3-2; Sulfolobus islandicus; RNA-Seq
Centrifugation at 10,000 rpm for 10 minutes to collect the cells, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols