16S rRNA amplicon metagenome sequencing: water microbial communities in Black Sea near Ochakiv (point 2) after Nova Kakhova dam explosion
DNA was extracted from water using DNeasy PowerSoil Pro Kit. V3-V4 regions of 16S rRNA gene were amplified with 515F/806R primers with Phusion High-Fidelity PCR Master Mix. Libraries were prepared using NEBNext Ultra DNA Library Pre Kit for Illumina. A nucleotide sequence was estimated on the NovaSeq 6000 platform.