GSM8275259: tp53 R242H/R242H 0 Gy, biol rep 1; Danio rerio; RNA-Seq
Each RNA sample was extracted from 50 zebrafish embryos anesthetized with tricaine (MS-222) and homogenized in 500μL TRIzol reagent (Life Technologies, 15596026) using a 1mL syringe and 23G needle. Lysed samples were purified and DNAse treated using the Direct-zol RNA MicroPrep kit (Zymo Research Corperation, R2060). 4ug of total RNA from each sample were sent for RNA sequencing. RNA Libraries were constructed for standard paired-read RNA sequencing by GeneWiz.