GSM7664050: w_day_7_exercised_4; Drosophila melanogaster; RNA-Seq
Legs from 15 flies were homogenized in the RLT buffer (Qiagen, Germany) with 1% beta-mercaptoethanol using a drill and incubated (55ºC, 10 min) with protein kinase K (Evrogen, Russia). Total RNA was extracted by a silica spin column (CleanRNA Standard, Evrogen, Russia). RNA concentration was evaluated by a fluorimetric assay (Qubit 4, Thermo Scientific). RNA integrity was evaluated in an aliquot of total RNA (200 μg) after DNase I (Thermo Scientific) treatment using capillary electrophoresis (TapeStation, Agilent, Germany). Strand-specific libraries were prepared by the NEBNext Ultra II Directional RNA Library Preparation kit (NEB, USA)