GSM7574831: HDM-treated B6 dorsal skin, replicate 1, scRNA-seq; Mus musculus; RNA-Seq
HDM-treated dorsal neck skin from 4 pooled Il31RAKO and 4 pooled WT C57Bl/6 mice was defatted, minced, digested (2 hours at 37*C in RPMI supplemented with Liberase TM, Hyaluronidase and DNAse), dissociated (GentleMACS (program C, Miltenyi), and filtered through 70 um metal mesh in preparation for flow cytometry. The live (DAPI-) CD45+ cell fraction of each sample (pooled B6 or pooled B6.IL31RAKO) was sorted using a Moflo XDP (Beckman Coulter) directly into ice-cold 0.5% BSA in PBS. 30,000 cells per sample were immediately processed through the Chromium Single Cell 3′ v2 Library Kit (10X Genomics) per the manufacturer's protocol by the UCSF Genomics Core Facility of the UCSF Institute for Human Genetics Single Cell 3′ libraries used standard Illumina sequencing primers for both sequencing and index reads and were run using paired-end sequencing with single indexing where Read1 is 26 cycles and Read 2 is 98 cycles.