GSM7664003: PHD2-WT-2; Homo sapiens; RNA-Seq
Total RNA from PHD2-deficient or wildtype H1299 cells was purified using the RNeasy Mini Kit (QIAGEN, 74104). Sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA, #E7530L) according to the manufacturer's protocol. Qualified libraries were pooled and sequenced on Illumina NovaSeq platforms using the PE150 strategy at Wuhan Benagen Technology Co., Ltd. (Wuhan, China).