GSM7748966: WT,sgGPATCH8,seqrun1,rep1; Homo sapiens; RNA-Seq
For cell line RNA sequencing (RNA-seq), RNA was extracted from K562 cells using the Qiagen RNeasy extraction kit, according to the manufacturer's instructions. A minimum of 500 ng of high-quality RNA (as determined by Agilent Bioanalyzer) per replicate was used as input for library preparation. Poly(A)-selected, strand-specific (dUTP method) Illumina libraries were prepared by the Integrated Genomics Operation (IGO) at Memorial Sloan Kettering with a modified TruSeq protocol and sequenced on the Illumina HiSeq 2000 to obtain ∼60-80M paired-end 100 bp reads per sample.