Long read transcript analysis of D. mel F1 progeny of a cross between strains 5905 and 11037: abdomen 3-8day post eclosion
Samples were collected in RNAlater. mRNA was isolated from approx. 20 abdomen samples using mRNA Direct Kit (Dynabeads) followed by full-length cDNA library construction using the Oxford Nanopore Kit SQK-PCB109 with barcoding. Barcoded samples were pooled and sequenced (ONT minion or promethion (UF ICBR). Fastq files were basecalled with Dorado (v2.1).