metagenomic sequence of rumen context:hu sheep
Approximately 200 mg of each sample was employed for DNA extraction. DNA was extracted via a bead-beating method using a mini-bead beater (Biospec Products; Bartlesville, USA), followed by phenol-chloroform-isoamyl alcohol extraction. Library preparation was performed according to the TruSeq DNA Sample Preparation Guide (Illumina, 15026486 Rev. C) method and procedure using 200 ng DNA as the template. Qualified libraries were selected for paired-ended sequencing on the Illumina NovaSeq 6,000 platform with a read-length of 150 base pairs (PE150).