GSM6813780: Control_3; Saccharomyces cerevisiae; OTHER
Cell pellets were lysed with dry ice in a commercial coffee grinder and chromatin domains affinity-purified as described in the manuscript. The purified chromatin circles were then treated with the M.CviPI/M.SssI (GpC/CpG-5-methyl-Cytosine-specific) and EcoGII (methyl-6-Adenine-specific) DNA methyltransferases. Methylated DNA was then isolated using Phenol-chloroform extraction.  EcoGII (methyl-6-Adenine-specific) DNA methyltransferases, MinIONTM Seqencing Library Preparation (Oxford Nanopore Sequencing) Nanopore long-read sequencing for direct detection of methylated bases