GSM6909675: A549-STAG2KO-rep3+Dex(100nM) [GR_24]; Homo sapiens; ChIP-Seq
Chromatin immunoprecipitation followed by sequencing (ChIP)-seq was performed as previously described (Schmidt, D. et al. Methods 48, 240–248 (2009).In brief, cells were fixed in 1% formaldehyde for 10 min and quenched with 0.125M glycine. Nuclear lysates were extracted as previously described and sonicated for 13 cycles (30 sec on/30 sec off) using the Bioruptor® Pico (B01060001, Diagenode). ). Per ChIP, 50 µL of magnetic Protein A beads (10008D, Thermo Fisher Sci-entific) beads were conjugated to 7.5µL of GR antibody, or 5µg of RAD21, PAXIP1, and H3K4me1 antibodies. Immunoprecipitated DNA was processed for library preparation usin KAPA library preparation kit, KK8234, Roche.