GSM7662985: p84-myc-PAK1; Homo sapiens; ChIP-Seq
Cells were treated with 1% paraformadehyde at room temperature for 20 mins. Quenched cell lysate was sonicated to generate DNA fragments using Bioruptor (Diagenode). Genomic DNA was prepared by treating aliquots of chromatin with Rnase, proteinase K and heat (overnight, 65 degrees C), followed by SPR1 bead clean up and quantified using a CLARIOstar microplate reader. An aliquot of chromatin was precleared with protein agarose beads. Genomic DNA regions of interest were isolated using antibody against myc-tag. Illumina sequencing libraries were prepared from the input DNA using standard enzymatic steps of end-polishing, dA-addition, and adapter ligation using an automated system (Apollo 342, Wafergen Biosystems/Takara).