GSM7401394: PBS; Mus musculus; RNA-Seq
Total RNA was extracted from sorted GFP+c-Kit+ CMM cells using Maxwell RSC simplyRNA tissue kit (Promega) Library was performed using Single Cell-3 Solution kit v3.1 following the manufacture's protocol. The final library size was determined using a High Sensitivity DNA Chip (Agilent). The libraries were sequenced with the paired end setting of 28 Read1 and 101 Read2 cycles, 10 index i7 and i5 cycles on NovaSeq 6000 platform (Illumina) using S4 Reagent kit v1.5 (Illumina).