GSM6381794: female, normal, large intestine, 12-week-old C57BL/6 mice; Mus; RNA-Seq
Single cells were partitioned into Gel Beads in emulsion using the GemCode instrument. Libraries were constructed using Single Cell 3' Library & Gel Bead Kit v2 and the A Chip Kit (10x genomics) according to the manufacturer's instructions. Briefly, cells were resuspended in the master mix and loaded together with partitioning oil and gel beads into the chip to generate the gel bead-in-emulsion (GEM).