MinION sequencing
Libraries for direct cDNA sequencing were prepared following the instructions in the direct cDNA sequencing with native barcoding protocol (SQK-DCS109 with EXP-NBD104) from Oxford Nanopore Technologies with minor modifications. Briefly, the VN Primer was replaced with a custom 3' cDNA RT primer (5_- ACTTGCCTGTCGCTCTATCTTCATTGATGGTGCCTACAG-3_, 2 ï¿½M).