RNA-seq of Citrus sinensis: leaf tissue
Cryoground leaves were used as input for RNA extraction. Strand-specific and barcode indexed RNA-seq libraries were generated following poly-A enrichment. Micro-capillary gel electrophoresis on a Bioanalyzer 2100 was used to verify the fragment size distribution of the cDNA libraries. The libraries were quantified using a Qubit fluorometer and pooled in equimolar ratios. qPCR was carried out to quantify the pooled libraries prior to sequencing on four lanes of an Illumina HiSeq 4000 (Illumina, San Diego, CA) with paired-end 150 bp reads.