DArTseq of Chenopodium quinoa:seedling
PstI and MseI compatible adaptors with two different restriction enzyme overhangs. The reverse adaptor contained the flowcell attachment region and MseI compatible overhang sequence. Only mixed fragments were effectively amplified in 30 rounds of PCR. After PCR, equimolar amounts of amplified product were bulked and subjected to 100 cycles of sequencing (single reads) on the Illumina NovaSeq sequencer