NextSeq 500 paired end sequencing; RNA-SEQ of epithelioid mouse mesothelioma treated with or without CSF1R inhibitor
RNA-SEQ of epithelioid mouse mesothelioma treated with or without CSF1R inhibitor
Tumours were harvested from the pleura of end-stage mice following humane euthanasia Tumours were initiated by administration of lentiviral vector carrying CRE recombinase to mice aged 8-10 weeks via single intrapleural injection of 10^7 viral particles per mouse. Asbestos (long fibre amosite) was administered via single intrapleural injection of 25ug fibres per mouse 10 days post CRE administration.  Mice were maintained until symptoms required humane killing. AZD7507 was dissolved in 0.5% HPMC + 0.1% Tween80 in dH2O and administered at 100mg/kg twice daily by oral gavage. Total RNA was isolated by Trizol according to the manufacturers instructions cDNA was generated using Illumina TruSeq library prep kit