GSM6631729: Escherichia coli K-12 tetracycline treated culture replicate 1 (Ultra directional library preparation); Escherichia coli str. K-12 substr. MG1655; RNA-Seq
Total RNA was extracted with TRIzol reagent (Thermo Fisher Scientific, Waltham, MA USA). The RNA samples were treated with a TURBO DNA-free™ kit (Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer's instructions. The RNA content was determined in a Qubit fluorometer (Thermo Fisher Scientific, Waltham, MA, USA). RNA integrity, purity, and concentration were assessed with a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). The Cappable-Seq procedure was performed as previously described [Ettwiller et al., 2016]. The Ultra directional libraries was prepared using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA, USA) library strategy: Cappable-Seq/Ultra directional