GSM7664100: Col-0, Ax, Rep3; Arabidopsis thaliana; RNA-Seq
Leaf samples collected from three biological replicates per treatment were flash frozen in liquid nitrogen and stored in -80C until processing. A biological replicate is defined as a pool of two leaves collected from individual plants in GnotoPots within the same microbox. Total RNA was extracted using the RNeasy Plant Mini Kit (Qiagen) according to manufacturer's protocol Stranded sequencing libraries were prepared using the TruSeq Stranded mRNA library preparation kit according to manufacturer's protocol.