GSM7765963: HEK293T_siSRSF1_10Gy_bio_rep3; Homo sapiens; RNA-Seq
RNA extraction was carried out as per Qiagen RNAeasy Mini Kit instructions for animal cells using vacuum technology. Samples were eluted in 30 μl RNase-free water and stored at -80°C until subsequent steps were performed. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.