WGS of multidrug-resistant STEC
Total gDNA was extracted using the Monarch HMW DNA Extraction Kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturers instructions. Genomic DNA was prepared for sequencing using the Oxford Nanopore (Oxford, United Kingdom). The sequencing library was prepared using the Rapid Barcoding Kit (RBK-114) according to the manufacturers instructions and sequenced on a PromethION with R10.4.1. Flow Cell (FLO-MIN114).