GSM8138135: HSPCs, barcode-derived cDNA; Homo sapiens; OTHER
We performed SC RNAseq on a sample form a healthy bone marrow donor using a pre-templated instant partitions (PIP) sequencing platform. Briefly, cryopreserved cells were thawed, normalized combined in a 1:10 ratio with barcoded PIPs, which were then vortexed and centrifuged into emulsifications. Emulsions were thermocycled, broken, mixed, washed, and pelleted. Reverse transcription was performed, and cDNA synthesis completed. After whole transcriptome amplification, PIPs were column filtered and purified with Ampure XP. Libraries were generated from WTA amplified material using the Nextera XT DNA Library Preparation Kit with a custom primer (PIPs_P5library) and standard Nextera P7 indexing primers (N70x). Libraries were pooled and sequenced using an Illumina NextSeq 2000.