Rattus norvegicus strain SHRSP/BbbUtx Iso-Seq derived from SHR-A3 male Lymph node
Total mRNA molecules were converted to cDNA using an oligo-dT primer, followed by template-switch oligo-directed second strand synthesis. Four barcoded cDNA samples were equimolar pooled for SMRTbell template preparation, as described by the manufacturer (Pacific Biosciences). Samples were sequenced on the Sequel IIe system, converted to circular consensus reads, demulitplexed, and initially processed with the IsoSeq3 pipeline for de novo prediction of full-length transcripts.