GSM7765090: H9 cells, KO_METTL3_1; Homo sapiens; RNA-Seq
Total RNAs were extracted from METTL3-SMASh tagged hNPCs (treated with or without ASV) using TRIzol Reagent (Invitrogen, 15596026). DNA digestion was car-ried out after RNA extraction by DNaseI. RNA quality was determined by examining A260/A280 with NanodropTM OneCspectrophotometer (Thermo Fisher). RNA Integrity was confirmed by 1.5% agarose gel electrophoresis. Qualified RNAs were finally quan-tified by Qubit3.0 with QubitTM RNA Broad Range Assay kit (Life Technologies, Q10210). 2 μg total RNAs were used for stranded RNA sequencing library preparation using KCTM Stranded mRNA Library Prep Kit for Illumina® (Wuhan Seqhealth, DR08402) following the manufacturer's instruction. PCR products corresponding to 200-500 bps were enriched, quantified and finally sequenced on Novaseq 6000 sequencer (Illumi-na) with PE150 model.