GSM7844573: M82  Rep2; Solanum lycopersicum; RNA-Seq
Total RNA were extracted from 1g of 4-week-old tomato fourth leaves with Nucleospin RNA kit (Macherey-Nagel), according to the manufacturer's instructions. RNA-seq libraries were prepared from 2μg of total RNA using NEBNext Ultra II Directional RNA library Preparation Kit (NEB) according to the manufacturer's instructions. RNA libraries were checked for quality and quantified using a 2100 Bioanalyzer (Agilent) and subjected to 2 × 150 bp high-throughput sequencing by NovaSeq 6000 (Illumina).