GSM6937711: Omentum tumor, Patient #5; Homo sapiens; RNA-Seq
The RNA was extracted according to the BGI's standard protocol. Briefly, main steps goes as follows: 1 Tissue Sample Fragmentation and Lysis; 2 Sample Purification with Chloroform/isoamyl alcohol and Isopropyl alcohol. Library was constructed according to the BGI's standard protocol. Briefly, main steps go as follows: 1 Sample QC; 2 rRNA Removal; 3 RNA Fragmentation; 4 cDNA synthesis; 5 End repair and Adaptor ligation; 6 PCR; 7 Library QC; 8 Sequencing.