Vibrio alfacsensis isolate:VA-1 Genome sequencing and assembly
We employed the BluePippin automated nucleic acid fragment recovery system for large DNA fragment retrieval, followed by end repair and barcode addition using the PCR-free EXP-NBD104 kit from Oxford Nanopore Technologies. Subsequently, fragment sizes were assessed using an AATI automated capillary electrophoresis instrument, and equimolar pooling of samples was performed. Finally, we utilized the SQK-LSK109 ligation sequencing kit from Oxford Nanopore Technologies to construct 1D libraries, which were subsequently sequenced on the Nanopore platform.