snRNA-seq of human cortical organoids
Each sample was derived from 30 pooled organoids, which were flash frozen in liquid nitrogen and stored at -80C until dissociation. Nuclei dissociation was performed as described in Matson et al., J. Vis. Esp, 2018 . Between 5,000  10,000 nuclei were inspected and counted on a hemacytometer before loading on to the 10X chromium controller (10x Genomics catalog number 1000171). Single nucleus RNA sequencing (snRNAseq) was performed using the 10X genomics Chromium Next Gem Single Cell Kit version 3.1 single index gene expression profiling assay according to the manufacturers instructions. Resulting libraries were tested on the 4200 TapeStation D1000 ScreenTape Assay (Agilent Biotechnologies) before paired-end sequencing on the Illumina NovaSeq 6000.