shotgun metagenomics of homo sapiens: child feces
Patient fecal samples were submitted to the Duchossois Family Institute at the University of Chicago (Chicago, IL, USA) for genomic DNA extraction (QIAamp PowerFecal Pro DNA Kit, Qiagen, Germantown, MD, USA), library generation (QIAseq FX Library Kit, Qiagen), and shotgun metagenomics sequencing on the Illumina NovaSeq platform (Illumina, San Diego, CA, USA) using the 2x150 Paired End read cassette.