EHF/ESE3 loss causes prostate epithelial cell plasticity and cancer (RWPE - RNA-seq data)
EHF/ESE3 loss causes prostate epithelial cell plasticity and cancer (RWPE - RNA-seq data)
Experiment in KSF medium. Cells were plated in T-25 flasks at 70% confluence. After 72 h, cells were collected and lysed with 500 µl TRIzol Reagent (15596026, Thermo Fisher Scientific). Experiment in EGM2-medium. Cells were plated in T-25 flasks at 70% confluence with EGM2 medium. After 72 h, EGM2 growth medium was replenished with fresh medium and after 96h  cells were collected and lysed with 500 µl TRIzol Reagent (15596026, Thermo Fisher Scientific). RNA was extracted using Direct-zol RNA MiniPrep kit (R2052, Zymo Research) following manufacture instructions. NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs Inc.) was employed with the NEBNext Multiplex Oligos for Illumina (New England BioLabs Inc.) and NEBNext® Poly(A) mRNA Magnetic Isolation Module for cDNA synthesis with the addition of barcode sequences.