Symbiodiniaceae of Platygyra daedalea
The Symbiodiniaceae ITS region was amplified using ITSintfor2 (5-GATTGCAGAACTCCGTG-3) (LaJeunesse and Trench, 2000) and ITS2-reverse (5-GGGATCCATATGCTTAAGTTCAGCGGGT-3) (Coleman et al., 2010). Specific primers were fused to sequencing platform primers. The final PCR products were sequenced using a paired-end (PE) 300 bp 2 strategy on an Illumina MiSeq sequencer operated by Sangon Biotech (Shanghai) Co., Ltd. (Shanghai, China).