GSM7766777: P024; Arabidopsis thaliana; RNA-Seq
Total RNA was isolated from these samples using the Qiagen RNeasy Plant Mini Kit (Cat no. 74904). Residual genomic DNA was removed using the Invitrogen Turbo DNA-free kit (Cat no. AM1907), according to the manufacturer's protocol. Libraries were prepared with the NEBNext Ultra II Directional Library Prep Kit for Illumina (Cat no. E7765), using the NEBNext poly(A) magnetic isolation module (Cat no. E7490). Quality control was performed with the Agilent 2100 Bioanalyzer instrument (Part no. G2939BA). Finally, a total of 70 libraries were pooled and sequenced, via Novagene, using one lane on an Illumina NovaSeq system.