GSM7294791: 33568_WT_ControlASO_500 ug_female; Mus musculus; RNA-Seq
Tissue samples were homogenized in Trizol using FastPrep beads on a Precellys 24 homogenizer. RNA was isolated and DNase treated on an RNeasy 96-well plate (Qiagen). Next generation sequencing libraries were generated using QuantSeq 3' mRNA-Seq Library Prep FWD Kit (015.96, Lexogen) according to the manufacturer's instructions.