GSM7928677: CUTTAG_CAL27_la; Homo sapiens; OTHER
Take out the cryovial and shake it in 37°C water to quickly thaw it within 1-2min.Cells were centrifuged for 5 min at 600×g at 4 ℃ Cell activity was detected with LUNA-FL and counted. The cells are bound to Concanavalin A-coated magnetic beads, and the cell membrane is permeabilized by Digitonin.The enzyme pA-Tn5 Transposase precisely binds the DNA sequence near the target protein under the antibody guidance and results in factor-targeted tagmentation