Targeted antibiotic resistance gene panel sequencing, gut
100 ng of the extracted DNA was used for library preparation using the KAPA HyperPlus Kit and KAPA HyperExplore MAX. The protocol included the following steps: library preparation, hybridization, bead capture, washing, amplification enrichment QC, sequencing, and pre- and post-capture multiplexing. The library underwent a final cleanup using the KAPA HyperPure Beads. The high-throughput sequencing of the obtained libraries was performed on MGI DNBSEQ-G400 platform using MGIEasy Universal Library Conversion Kit (App-A), High-throughput Sequencing Primer Kit-C (App-C), DNBSEQ-G400RS High-throughput Rapid Sequencing Kit (FCS PE100) and DNBSEQ-G400RS Rapid Sequencing Flow Cell (FCS).