Cut and Run seq: MEKi rep1
Cells were nuclei extracted with nuclei extraction buffer (20mM HEPES pH 7.9, 10mM KCl, 0.1% Triton X-100, 20% Glycerol and 1mM MnCl2). Cut and Run were performed and DNA were spike-in with Ecoli.CUT& RUN libraries were prepared with 10ng CUT& RUN DNA using NEBNext Ultra II DNA library prep kit (NEB)