GSM6454727: Left ventricle, DOCA-salt HTO; Mus musculus; RNA-Seq
Left ventricular tissue was perfused with PBS.Tissue was minced with fine scissors into small pieces and digested with Liberase TM for 30 minutes at 37 degrees C. Immune cells were then enriched for using magnetic beads toward CD45 (Miltenyi). Resulting samples were then stained with hashtags and extracellular antibodies. Finally samples were ashed and experimental groups pooled. Cells were then sorted for live single CD45+ cells and loaded onto the 10X controller. Libraries were prepared according to manufacturer's instructions for 10X Chromium Single Cell 5' Reagent Kit v2 with the 5' Feature Barcode Library Kit by the Vanderbilt Technologies for Advanced Genomics core targeting 20,000 cells/lane and sequenced on a NovaSeq 6000 targeting 50,000 reads per cell