GSM7092111: BACH1_D6; Mus musculus; OTHER
Cut&Tag Operate through cell permiabilization with 0.05% Digitonin. Tn5 Transposase cleaves DNA and addition of 6.67 µL 0.5M EDTA, 2 µL 10% SDS and 1.67 µL 20 mg/mL Proteinase K, Incubation at 50C for 1hr and 37C overnight to extract DNA NEB High Fidelity PCR MasterMix