GSM7576428: 8W_atac_R1; Mus musculus; ATAC-seq
A total of 100000 GFP positive cells isolated by FACS from gonads at E14.5, 1W and 8W (three biological replicates per timepoint) were used for ATAC-Seq library preparation using Active Motif ATAC-Seq Kit (cat. number 53150). Libraries were prepared using the  KAPA Real-Time Library Amplification Kit following the recommendations of the ATAC-Seq kit by Active Motif Tagmented DNA was purified using DNA purification columns and library amplification was performed using Illumina's Nextera adapters. A unique combination of i7/i5 primers was used to allow for multiplexing and sequencing on the same flow cell. PCR amplification included 5 minutes incubation at 72°C, followed by 30 sec of DNA denaturation at 98°C and 10 cycles of: 98°C for 10 sec, 63°C for 30 sec and 72°C for 1 minute. PCR products were cleaned up using SPRI beads and size distribution of PCR amplified libraries was assessed on Tapestation.