GSM8138397: Tumor, UPN-574, Post-treatment, Tumor_Cells_CD45_positive, F05560-GEX_F05627-TCR; Homo sapiens; OTHER
GentleMAC dissociation using Miltenyi Tumor Dissociation Kit human (Order no. 130-095-929). CD45 positive cells were separated using Miltenyi CD45 (TIL) MicroBeads human (Order no. 130-118-780). RNA was extracted via single cell GEM reactions using 10x Chromium next GEM single cell 5' reagent kit v2 (dual index) protocol according to manufacturer recommendations (CG000330revA). CAR construct specific primers were spiked in during GEM generation. TCR libraries were generated using the 10x Chromium platform 5′ v2 library preparation kits (10x Genomics) according to the manufacturer's recommendations and targeting 5,000 to 10,000 cells per sample.