GSM7575053: HT29 cells, shNFIB-991-miRNA; Homo sapiens; miRNA-Seq
Total RNA from NFIB-knockdown HT29 or control cells was extracted and sequenced by Vazyme Corporation (Nanjing, China) to reveal differentially expressed mRNAs, lncRNAs, circRNAs, and small RNAs. After removing rRNA using the Ribo-Zero kit (Illumina, USA), the cDNA library was constructed using VAHTSTM DNA Clean Beads (Vazyme, China). The final libraries were quantified using an Agilent 2100 Bioanalyzer (Agilent, USA) and an ABI Step One Plus Real-Time PCR system. The libraries were then sequenced on a HiSeq 2500 platform (Illumina, USA). For small RNA sequencing, small RNA was isolated from total RNA using 15% PAGE. The small RNA library was constructed using the TruSeq Small RNA Sample Preparation Kit (Illumina, USA). The libraries were then sequenced on a Hiseq 2500 platform (Illumina, USA) using a synthetic method.