GSM7663617: E10.5 otocyst SOX2, CUT&Tag; Mus musculus; OTHER
Cells were equally divided into three samples and subjected to CUT&Tag. Cells were incubated with either anti-CHD7 or anti-SOX2 antibodies, while the no primary antibody control did not receive any antibodies. DNA libraries were prepared from samples and 150 bp paired end sequencing performed on an Illumina NovaSeq6000 S4 Flowcell at the University of Michigan Tn5-mediated tagmentation using the CUT&Tag-It kit (Active Motif).