GSM7900423: Untreated, Day 3, Clone B; Homo sapiens; OTHER
Flow cytometry and PCR amplification of genomic sgRNA sequences were conducted as previously described in detail in https://doi.org/10.1016/j.celrep.2020.108541. 10 sgRNAs were designed for each TSS of hg19 annotated lncRNAs expressed in THP1s at baseline and upon stimulation. The sgRNA library also included 700 non-targeting control sgRNAs, and sgRNAs targeting 50 protein coding genes as positive controls. The sgRNA library was designed and cloned as previously described in DOI: 10.1126/science.aah7111 Guide-Seq