GSM6594947: TN CD8+ T cells, dirty, timestamped at day 28, rep 3 (RNA-seq); Mus musculus; RNA-Seq
10,000 FACS sorted cells were placed in Trizol and RNA was purified following the manufacturer's instructions, with the addition of a second chloroform extraction, GlycoBlue carrier when precipitating, and a second wash of the pelleted RNA in 70% ethanol. RNA integrity was confirmed on a Fragment Analyzer. Libraries were prepared by the TREx core at Cornell University using the NEBNext Ultra II RNA Library Prep Kit (New England Biolabs).