GSM7092032: muscle, polymyositis patient 2; Homo sapiens; OTHER
The IIM biopsy tissue was prepared for sequencing by first isolating RNA from frozen sections using the Qiagen RNA Mini kit according to the manufacturer's instructions. B cells were isolated from frozen PBMCs using a human Pan B immunomagnetic negative selection kit (StemCell). IBM RNA libraries were made using reagents supplied by New England BioLabs as part of the NEBNext Immune Sequencing Kit. B cells were loaded directly into the Chromium Controller (10x Genomics) and prepared using the Chromium Single Cell 5′ Reagent Kit (10x Genomics) for version 3 chemistry per the manufacturer's protocol.