GSM8086562: SJ049_CTRL; Homo sapiens; RNA-Seq
PDX cells from individual treated and untreated mice were fixed separately using the 10X Genomics CG000478 Demonstrated Protocol Cell & Nuclei Fixation Chromium Fixed RNA Profiling Rev B. After fixation, individual samples were counted and target genes were barcoded with unique sample barcodes during probe hybridization. Uniquely barcoded samples were counted using Trypan Blue stain and then pooled in equal numbers following 10X Genomics CG000565 Chromium Fixed RNA Profiling Multiplexed Samples Pooling Workbook Rev B. Pooled cells were washed and 8000 cells were targeted from each sample to be partitioned into GEMS on Chip Q using the 10X Genomics Chromium Chromium Fixed RNA Profiling kit. Partitioned cells underwent barcoding, reverse transcription, cDNA amplification and sample index ligation following the 10X Genomics CG000527 Chromium Fixed RNA Profiling Mouse/Human Chimera Isolation kit (Stemcell Technologies, 19849) Libraries were prepared following 10x Single Cell Gene Expression Flex Fixed RNA Profiling (FRP) protocol and sequenced on Illumina NovaSeq 6000 149 system  (Illumina). The Single Cell Gene Expression Flex Fixed RNA Profiling (FRP)