GSM7574847: RLE-6TN cells, ChIP-1-Input; Rattus norvegicus; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes to preserve the protein-DNA interactions.And lyse the cells to release the chromatin.Fragment the chromatin into smaller pieces using sonication or enzymatic digestion. This step is crucial to obtain DNA fragments of the appropriate size for sequencing. ChIP-Seq libraries were prepared with the KAPA HTP Library Preparation Kit complemented with NEXTflex DNA Barcodes from Bio Scientific.