Male_Pacbio
Thelibrarywasconstructedthrough:(1)gDNAshearing,(2)DNAdamagerepair,(3)blunt_end ligation with hairpin adapters from the SMRTbell Template Prep Kit 1.0 (Pacific Biosciences), (4)size selection, and (5) binding to polymerase. A total 5 g DNA per sample was used for the DNA library preparations.