GSM7664008: Young.; Mus musculus; RNA-Seq
For the young and old pituitary sample, anesthetized mice were perfused via the aorta with cold PBS and dissected pituitary was placed into an enzyme mix containing 0.5% w/v collagenase type 2, 0.25% trypsin, in Hanks balanced salt solution for 2-3 hours at 37°C. The tissue and cells were gently triturated and pelleted at 900g for 3 minutes, and resuspended for further single cell sorting. All live cells from young or old pituitary negative for DAPI  staining were sorted for further scRNA-seq. Libraries were prepared for sequencing using standard Illumina protocols