GSM7844408: WT 1 iWAT; Mus musculus; RNA-Seq
RNA was extracted from frontal brain, lumbar spinal cord, BAT, iWAT, TA muscle and liver of wild type and homozygous FUSΔ14/Δ14 male mice at 9-weeks of age. Quality and quantity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample The clustering of the index-coded samples was performed on a cBot Cluster Generation System using PE Cluster Kit cBot-HS (Illumina) according to the manufacturer's instructions. After cluster generation, the library preparations were sequenced on an Illumina platform and paired-end reads were generated. Total number of reads was 30 million per sample.