GSM7399288: Memory CD4 T cell gene expression from macaque T034 PBMC, plate7 wellD1; Macaca mulatta; RNA-Seq
Single cells in 96-well plates were lysed in SMART-Seq lysis buffer supplemented with RNAse inhibitor for 2 minutes at room temperature, vortexed, centrifuged, and frozen at -80C until cDNA library preparation. Whole cDNA library was prepared using the SMART-Seq Ultra Low Input RNA kit.