GSM8229792: RC_PBS_2; Mus musculus; RNA-Seq
Mice were euthanized with CO2 and brains quickly removed. Single cells were dissociated using Miltenyli Adult Brain Dissociation Kit, and Myelin Removal Beads. Libraries were prepared using Chromium Next GEM Single Cell 3' Reagent Kits v3.1 per manufacturer's instructions, and targeting 5,000 cells. 10xGenomics Libraries: (protocol - Chromium Next GEM Single Cell 3', reagent Kit v3.1), Ribo-Zero GOLD kit