GSM8182874: BT67 cells,siPTBP1_3,Day3; Homo sapiens; RNA-Seq
Total RNA from cells was extracted by an RNA isolation kit (Vazyme, China,Cat:RC112-01) according to the manufacturer's protocol.Qualified RNAs were quantified by Qubit3.0 with QubitTM RNA Broad Range Assay kit (Life Technologies, Q10210).2 μg of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using KC-DigitalTM Stranded mRNA Library Prep Kit for Illumina® (Catalog NO. DR08502, Wuhan Seqhealth Co., Ltd. China) following the manufacturer's instruction.