GSM7765134: CD8+ T cell scr No stim Rep1; Mus musculus; RNA-Seq
Total RNA was extracted using the Qiagen Rneasy Plus Universal Mini Kit per manufacturer's instructions (Qiagen, Hilden, Germany). RNA was checked for quantity and integrity, then libraries were prepared using the NEBNext Ultra II RNA Library Prep for Illumina as per manufacturer's instructions (New England Biolabs, Ipswich, MA, USA). Briefly, Oligod(T)-enriched mRNA was fragmented for 15 mins at 94°C and first and second strand cDNA subsequently synthesized.