GSM6373973: CRC264_Mir_n3; Homo sapiens; RNA-Seq
Cell pellets of growing spheres were lysed and RNA was extracted with Maxwell RSCmiRNA tissue kit (Promega) according to manufacturer's instructions. Total RNA (100 ng) from each sample was prepared using QuantSeq 3'mRNA-Seq Library prep kit  (Lexogen, Vienna, Austria) according to manufacturer's instructions The amplified fragmented cDNA of 300 bp in size were sequenced in single-end mode using the Illumina NovaSeq 6000 with a read length of 100 bp