GSM7844462: iPSC-derive microglia,  10K biol rep 1, ATAC; Homo sapiens; ATAC-seq
Samples that yielded the best fragmentation started from a total of 10K, 31K, and 100K cells. Cells were processed according to the Buenrostro et al. 2015 protocol with modifications published by the Kaestner Lab that include the lysis step and transposition buffer published in the Omni ATAC protocol. Breifly, cells were lysed and tagmented using illumina reagents.We also used magnetic beads for purification of libraries. For library generation, Illumina Nextera DNA unique Dual Indexes plus NEBNext High-Fidelity 2X PCR Master Mix was added to purified transposed DNA. PCR amplified libraries were size selected with KAPA Pure beads. The quality and quantity of libraries were assessed using a combination of Agilent DNA High Sensitivity chip and QuantFluor dsDNA System.