RNA-seq of Rhododendron simsii red flower
Total RNA was isolated using TRIzol kit (Takara). RNA was treated with DNase I, enriched with magnetic oligo(dT) breads, and fragmented by fragmentation buffer. After synthesis of first-stranded cDNA synthesis, RNase H and DNA polymerase I-mediated second stand synthesis, double-stranded cDNAs purification, end repaired, and added with poly (A), PCR amplification (25 cycles) was carried out. Following verification of quality and quantity, the cDNA libraries were sequenced. Raw reads were subjected to bioinformatic pipeline.