GSM6909167: 0041; Bos taurus; RNA-Seq
Total RNA was isolated from 15 heifers using Trizol reagent (Invitrogen, Carlsbad, CA, USA) following standard procedures. RNA purification and DNase digestion were done using an RNA clean and concentrator kit (Zymo Research, Irvine, CA, USA). The quality and RNA integrity of total RNA was assessed using Agilent Bioanalyzer, following the Agilent RNA 6000 Nano kit (Agilent, Santa Clara, CA, USA) instructions. Library preparation and sequencing were performed on the Nova-Seq platform at the Discovery life sciences (Hudson Alpha Institute of Biotechnology, Huntsville, AL, USA). Paired end 100 bp reads were generated for each sample.