H3K79me2 ChIP-seq on HPCHOXB4
Cells were fixed with 1% formaldehyde according to the Active Motif ChIP cell fixation protocol. Fixed cell pellets were submitted to Active Motif for ChIP-seq analysis. Briefly, 15ug of chromatin and 10uL of H3K79me2 antibody (Active Motif, #39143) were used for each IP reaction. Illumina base-call data were processed and demultiplexed using bcl2fastq2 v2.20 and low-quality bases with Phred scores less than 33 were trimmed. 75 bp single-end sequence reads were subsequently mapped to the genome through BWA v0.7.12 algorithm with default settings. Low quality reads were filtered out and PCR duplicates were removed.