GSM7574439: HA ChIP-Seq; Mus musculus; ChIP-Seq
For ChIP-seq, cells were crosslinked with 1% formaldehyde for 10 min at room temperature and quenched with 0.125 M glycine. The final DNA length was approximately 300 bp sonicated using a COVARIS S220. For IP, 1/10 of the supernatant was incubated with the HA (CST, 3724) or p53 (CST, 2524) antibody at 4 °C overnight and immunoprecipitation was performed with Protein G magnetic beads. Immunoprecipitated chromatin (ChIP DNA) was eluted in TE buffer. Following end-repair, A-tailing, adaptor ligation, library amplification was performed with the VAHTS Universal DNA Library Prep Kit for Illumina kit (Vazyme, ND607).