GSM7899161: ATAC_MG_oxLDL; Homo sapiens; ATAC-seq
50,000 ECFCs were trypsinized and washed twice in cold PBS. Pelleted cells were lysed and then directly transposed using the TDE1 DNA Enzyme and Buffer kit (Illumina, CA, US). The transposition reaction was completed with thermomixing at 37°C for 30 minutes at 300 rpm. Transposed DNA was purified with MinElute column cleanup (Qiagen, Valencia, CA) Prepared libraries were purified with MinElute column clean-up (Qiagen). The ATAC-seq library pool was quantified on the Agilent Bioanalyzer with the High Sensitivity DNA kit (Agilent Technologies, 4067-4626). Sequencing was performed using the Illumina NextSeq500 (NextSeq control software v4.0.0 / Real Time Analysis v2.11.3). The library pool was diluted and denatured according to the standard NextSeq protocol (Document # 15048776 v16) and sequenced to generate paired-end 76 bp reads using a 150 cycle NextSeq500/550 Mid Output reagent Kit v2.5 (Illumina, 20024904). After sequencing, fastq files were generated using bcl2fastq2 (v2.20.0.422).