GSM7401063: Tel M 318; Gasterosteus aculeatus; RNA-Seq
Sixty minutes after their final trial (n=4 males after a courtship opportunity, n=5 males after a territorial intrusion, n=4 males after the tradeoff treatment, and n=4 males after the control treatment), the diencephalon and telencephalon were rapidly dissected, preserved in RNAlater, and deep frozen at -80°C until extraction. RNA was extracted using Invitrogen PureLink RNA extraction kits (Invitrogen Corporation, Carlsbad, CA) and quality was checked using Agilent Bioanalyzer chips. Libraries were prepped using TruSeq Stranded mRNA sample prep kits and samples were sequenced to a depth of >30M reads on a Novaseq 6000 S4 flow cell