16S sequencing of human stool: cryptosporidium positive
DNA was extracted from stool samples with a QIAmp Fast DNA Stool mini kit (Qiagen, Germany) and the V3-V4 hypervariable region was amplified with a 2KAPA Hifi HotStart ready Mix (KAPA Biosystems Inc., Wilmington, MA, USA) according to the manufacturers instructions. Illumina Nextera adaptor primers were used to index the samples, after which they were sequenced on an Illumina MiSeq Platform.