homo sapiens: adult male whole blood
After RNA isolation and globin depletion, a total of 36 RNA samples (RIN > 7, 260/280 > 1.8) isolated from PAXgene tubes collected at Day of Surgery (DOS) and Post-Operative Day 1 (POD1) from 18 patients (8 cases and 10 matched controls) passed the RNA integrity quality controls and were used for RNA-seq. A total of 100ng of RNA were used to construct libraries, and sequencing was performed using an Illumina HiSeq 4000 at the WCM Epigenomics Core Facility using standard Illumina protocols.