GSM7662539: Control; Timepoint-2; 22-P-1120 [22_P_1120_2_t]; Homo sapiens; RNA-Seq
We collected blood samples using a procedure of capillary puncture before (Time Point 1) and after (Time Point 2) exposure to music, including 16 ACD patients, and 14 healthy donors. Sample was collected in a tube containing the proper volume of PAXgene additive. RNA was isolated using the PAXgene blood miRNA extraction kit (Qiagen). On-column DNase I treatment was carried out during the extraction process. RNA concentration step and an additional DNase treatment were undertaken using an RNA clean & concentrator kit (Zymo Research) prior to sequencing Strand specific library preparation was completed using the SMARTer Ultra low input RNA library (Poly-A). Sequencing was performed using a NovaSeq 6000 System at 100 paired end configuration to finally obtain 70M reads per sample