GSM8138442: TEB-007;N1; Homo sapiens; RNA-Seq
The nasal scrapings were immediately transferred to lysis buffer (RLT buffer, RNeasy micro kit, Qiagen, Germany) and kept on ice until total RNA was isolated using the protocol as described by the manufacturer. The RNA quality and concentration was determined by 2100 Bioanalyser instrument (Agilent). Sequencing libraries from total RNA were prepared at the Core Facility Genomics, Medical University of Vienna, using the QuantSeq FWD protocol (Lexogen). 21 PCR cycles were used for library prep, as determined by qPCR according to the library prep manual. Libraries were QC-checked on a Bioanalyzer 2100 (Agilent) using a High Sensitivity DNA Kit for correct insert size and quantified using Qubit dsDNA HS Assay (Invitrogen). Pooled libraries were sequenced on a NextSeq500 instrument (Illumina) in 1x75bp single-end sequencing mode.