4-week PFC Akap11 -/- snRNA-seq Sample 3
Nuclei extraction was performed within 16 hours of brain tissue dissection to avoid freeze-thaw cycles, which would result in poor quality nuclei. A gentle, detergent-based dissociation was used to extract the nuclei, according to a previously published protocol available at protocols. io (https://www.protocols.io/view/frozen-tissue-nuclei-extraction-bbseinbe). Extracted nuclei were loaded into the 10x Chromium V3.1 system (10x Genomics) and library preparation was performed according to the manufacturers protocol.