GSM8278704: E-hASCs, replicate 1, scRNAseq; Homo sapiens; RNA-Seq
The hASCs were isolated with 0.1% collagenase I from the abdominal subcutaneous fat (SAT) of 3 healthy infants and 3 elderly. Single-cell suspensions of primary hASCs with PBS were loaded onto microwell chip using the Singleron Matrix®Single Cell Processing System. Barcoding Beads are collected from the microwell chip, and mRNA captured by the Barcoding Beads was reverse transcribed into cDNA, followed by PCR amplification for the library construction. The scRNA-seq libraries were constructed based on the protocol of the GEXSCOPE® Single Cell RNA Library Kits. Individual libraries were diluted to and pooled at a concentration of 4nM, and subsequently sequenced on Illumina novaseq 6000 with 150 bp paired end reads.