16s rRNA of feces: juvenile rat
Sequencing was performed using an Illumina MiSeq sequencer with 2 x 300 cycle MiSeq Reagent Kit v3 (Illumina). The adapter sequences and low-quality regions were trimmed by Cutadapt and Trimmomatic, respectively. Further data processing was performed using the open-source software pipeline QIIME2 version 2019.4.0. Reads were trimmed of primers and sequence quality control was performed using QIIME2s script. Amplicon Sequence Variants (ASVs) were formed using DADA2 (denoise-paired). The phylogenetic assignment of representative sequences from each ASV was carried out with a 16S reference dataset obtained from Greengenes version 13_8.