GSM6594854: RNA Cebpa-/p30 Tet2-/- s7; Mus musculus; RNA-Seq
RNA was isolated from 5*10^5 sorted cells using RNeasy Mini Kit (#74104 Qiagen) according to the manufacturer's instructions and quality was assessed by RNA 6000 Pico Kit (#5067-1513 Agilent) on a Bioanalyzer 2100 (Agilent). 200 ng RNA was used to generate sequencing libraries using TruSeq RNA Library Prep Kit v2 (#RS-122-2001 Illumina), the libraries were quantified using QubitTM dsDNA BR Assay Kit (#32853 Thermo Fisher Scientific) and size distribution assessed by DNA 1000 Kit (#5067-1504 Agilent) on a Bioanalyzer. Multiplexed libraries were sequenced on a NextSeq 500 (Illumina) using NextSeq 500 High Output v2 Kit (75 cycles; #FC-404-2005 Illumina).