MinION fastq for Oscheius tipulae CEZ-107
Worms were washed for 1 hour then pelleted and lysed in 5x volume lysis buffer (200 mM NaCl, 100 mM Tris-HCl pH 8.5, 50 mM EDTA pH 8, 0.5% SDS, 0.1 mg/mL freshly thawed proteinase K) overnight at 65C, then for 20 minutes at 95. 0.1 mg/mL RNase A was added and incubated at 37 for one hour. DNA was extracted by phenol/chloroform/isoamyl alcohol and ethanol precipitation. Size selection was performed with Circulomics Small Read Elimination kit, and DNA was prepared for MinION sequencing according to the manufacturers instructions.