GSM8182202: MDM, untreated, rep1; Homo sapiens; RNA-Seq
Total RNA was obtained using Direct-zol™ RNA Miniprep. RNA samples were treated with a DNase I column to remove contaminating genomic DNA. RNA was quantified by spectrophotometry, and 1μg of RNA was used for bulk RNA-Seq. The sequencing library is prepared by random fragmentation of the DNA or cDNA sample, followed by 5' and 3' adapter ligation. Alternatively, "tagmentation" combines the fragmentation and ligation reactions into a single step that greatly increases the efficiency of the library preparation process. Adapter-ligated fragments are then PCR amplified and gel purified.