BCR AIIMS 330 2018 heavy chain data
Total RNA from 2 or 3 million PBMCs was extracted (RNeasy Maxi Kit, Qiagen) from each time point and antibody sequences were amplified using methods and primers as previously described. The PCR product sizes were verified on agarose gel (E-Gel EX; Invitrogen) and quantified with fluorometry (Qubit; Life Technologies), pooled at approximately equimolar concentrations and each sample pool was re-quantified before sequencing on an Illumina MiSeq