GSM6692751: TL-21-FDHQB2HP; Homo sapiens; RNA-Seq
RNA extraction was performed by Tempus, Inc. as per their protocol detailed here: https://doi.org/10.18632%2Foncotarget.25381. Briefly, FFPE tissue was digested with proteinase K and total nucleic extracted using a Chemagic360 instrument. RNA was isolated using DNAse digestion followed by magnetic bead purification. RNA library creation was performed by Tempus, Inc. as per their protocol detailed here: https://doi.org/10.18632%2Foncotarget.25381. Briefly, RNA was fragmented with heat and cDNA amplified using random primers. Next, A-tailing, adapter ligation, bead-based cleanup, and library amplification was performed. Samples were then hybridized with the IDT xGEN Exome Research Panel and recovered using Streptavidin-coated beads, followed by amplification using the KAPA HiFi Library Amplification Kit.