GSM8277318: INTS11DEG_RNA_0h_rep2; Mus musculus; RNA-Seq
Total RNA was extracted from freshly collected time-course samples using TRIzol reagent and ethanol precipitation as per the guidelines provided by the manufacturer. The obtained total RNA underwent Turbo DNase treatment to eliminate any genomic DNA contamination. Subsequently, RNA-seq libraries for sequencing were generated using the TruSeq Stranded Total RNA library preparation kit (Illumina). One μg of DNase-treated input RNA was utilized for library construction, and the ERCC RNA Spike-In Mix (Invitrogen) was incorporated following the manufacturer's instructions. The constructed libraries were pooled and subjected to single-end sequencing with 75 base pairs read length on a NextSeq 500 platform.