Metabarcoding of prokaryotes in marine sediments
DNA extraction with ZymoBIOMICS DNA Miniprep Kit, amplification of the V3-V5 hypervariable regions of the 16S gene done using the primer pairs 515F_ILMN (5- GTGYCAGCMGCCGCGGTA-3) and 909R_ILMN (5- CCCCGYCAATTCMTTTRAGT-3)