single-cell RNA-Seq of homo sapiens: adult female high grade serous carcinoma tumor
Tumor tissues were dissociated, frozen, thawed and labeled with TotalSeq-B antihuman antibody-oligonucleotide conjugates from BioLegend. 4 sets of samples were pooled and prepared for sequencing using the 10X Genomics 3' CellPlex Kit and sequenced on an Illumina NovaSeq 6000 system using the S2 Reagent Kit v1.5 (100 cycles).