PfHDAC1 ChIPseq Troph Stage under DHA
ChIP-sequencing libraries were prepared from 5-10 ng of DNA using the NEBNext Ultra II DNA Library Prep kit. Chromatin immunoprecipitated, fragmented DNA samples were end repaired and adapters ligated. Size selection was performed using Agencourt XP beads (Beckman Coulter). Adapter ligated fragments were PCR amplified using indexing primers