GSM7662913: HEC1B siPRMT6-1 48hr H3K27ac; Homo sapiens; ChIP-Seq
Cells were fixed with 0.5% formaldehyde for 10 min at room temperature, quenched with 1.25 M glycine, washed, and lysed. The chromatin was sheared using S220 Focused-ultrasonicator (Covaris). ChIP-seq libraries were prepared using ThruPLEX DNA-seq Kit (Takara)