GSM6935775: Control replicate 2; Mus musculus; RNA-Seq
2cm distal colon segments were taken from mice after confirmation of death, and placed into 1 ml of RNA later. RNA was extracted with Qiazol reagent (Invitrogen). Distilled solutions of RNA were purified using chloroform and isopropanol and eluted with ethanol before being resuspended in RNAse free water. RNA was cleaned up using the Qiagen RNeasy Microkit according to the manufacturer's guidelines. Libraries were prepared using 10x Chromium Single Cell 3' Reagent Kits (v3)