GSM8023222: Sample_4; Homo sapiens; RNA-Seq
Total RNA was isolated from the collected samples using the miRNeasy Tissue/Cells Advanced Kits (Qiagen) following standard manufacturer's protocol. RNA concentration and integrity were measured on a Agilent 2100 Bioanalyzer (Agilent Technologies). RNA samples with RNA integrity number (RIN) over 7 were considered for RNA sequencing. The RNA samples were processed for directional RNA-seq library construction using the Illumina Total RNA Prep with Ribo-Zero Plus library preparation kit according to the manufacturer's protocol. We performed  100 nt paired-end sequencing using an Illumina Novaseq 6000 platform and obtained ~80 million clusters per sample with 92% >Q30.