Shotgun sequencing of UF42971
DNA from muscle tissue was extracted using a Qiagen DNeasy Blood & Tissue Kit following the protocol supplied by the kit. DNA libraries were produced for these samples with a xGen DNA Lib Prep EZ. The enzymatic shearing was set to 13 minutes to produce 350 bp fragment sizes according to manufacturer's guidelines. Library amplification PCR steps were 16. Samples were cleaned, pooled, and shotgun sequenced on 40% of an Illumina NovaSeq, S4, using a 2x150 platform.