16S rRNA sequencing of Aleurocanthus camelliae
3rd larva stage were starved for 6h and then were surface sterilized with 75% ethanol for 2 min and rinsed with distilled sterile water three times before DNA extraction.The V4 region of bacterial 16S rRNA genes was amplified from the DNA extracts using the bacterial-specific primers (V4-515F: 5' -GTGCCAGCMGCCGCGGTAA -3', V4-806R: 5'-GGACTACHVGGGTWTCTAAT-3'). PCR products were purified using a Gel Extraction Kit (Qiagen, Germany) and then pooled in equimolar concentrations. Libraries were qualified by the Agilent 5400 bioanalyzer (Agilent, United States)