FATHER
Whole genome long read (LR) sequencing libraries were generated using the Oxford Nanopore technology (ONT) ligation sequencing kit and then sequenced with the PromethION Beta platform. Sequencing depth was calculated on the resulting alignments using mosdepth v0.2.38 with the parameters -F 3588 -Q 1, which calculated coverage of depth in 100 bp bins and included only primary and supplemental alignments. Alignment of long read DNA sequencing was performed with NGMLR v0.2.79 using default parameters along with the -bam -fix parameter for long CIGAR string support. Average coverage of 30x was achieved for the trio.