RNA-Seq of total RNA extraction of Aphrocallistes beatrix holobiont
Ribosomal RNA (rRNA) was depleted using the RiboMinus kit with a mix of Eukaryote and Prokaryote probes (Thermo Fisher Scientific) according to manufacturers instructions, followed by Illumina NextSeq library construction. Sequencing was conducted on an Illumina NextSeq platform with 150 bp paired-end chemistry on a MID flowcell at the Ramaciotti Centre for Genomics (UNSW, Australia).