GSM7899185: RNA_MG_veh; Homo sapiens; RNA-Seq
RNA was extracted using Rneasy mini plus kit (Qiagen) RNA-Seq libraries were prepared using the Illumina stranded total RNA prep ligation with Ribo-Zero plus kit (Illumina, 20040529) and IDT for Illumina RNA UD Indexes (Illumina, 20040553/20040554/20040555/20040556) according to the standard manufacturer's protocol (Illumina, Document # 1000000124514 v01).The libraries were quantified on the Perkin Elmer LabChip GX Touch with the DNA High Sensitivity Reagent kit (Perkin Elmer, CLS760672). Libraries were pooled in equimolar ratios, and the pool was quantified by qPCR using the KAPA Library Quantification Kit – Illumina/Universal (KAPA Biosystems, KK4824) in combination with the Life Technologies Viia 7 real time PCR instrument.