GSM8182631: KP1-59185; Mus musculus; RNA-Seq
Mouse tumor fragments were immediately frozen in liquid nitrogen after isolation. RNA was isolated using the RNeasy Mini Kit (Qiagen), and RNA integrity (RIN) was determined by Bioanalyzer (Agilent). All samples exhibited acceptable quality (RIN > 7) for RNAseq with polyA capture. NGS libraries, prepared according to the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolab) (version 4.0, 4/21) manual by MARGenomics Core Facility at Hospital del Mar Research Institute, yielded a final library size of ~40M counts per sample. Sequencing was conducted on the Illumina Nextseq 500 High-output platform with 2x75bp read length at the UPF Genomics Core Facility.