GSM7050931: PT_6, lymph_node_sc; Homo sapiens; RNA-Seq
Cells suspension from primary tumor and paired lymph node metastasis samples were loaded into the 10X Chromium Controller using the Chromium 10×Genomics Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1. Fresh frozen primary tumor samples slide gene expression were detected by 10X Visium Spatial Gene Expression Slide & Reagents Kit. RNA libraries were prepared for sequencing using 10x chromium single cell 3' Reagent Kits v3.1 protocols or 10x Visium spatial protocols and standard Illumina protocols