GSM7928758: cDNA from Patient-derived CTCs from BC patient 001 (timepoint 3, exp 2); Homo sapiens; RNA-Seq
Using CellCelector, an automated single-cell picking system (ALS), pools of single CTCs, CTC clusters and CTC–WBC clusters (range of 5–40 cells) were collected and immediately transferred into tubes (Axygen, 321-032-501, Thermofisher AB-0620) containing 10 µl RLT Plus lysis buffer and 1 U SUPERase IN RNase inhibitor (Invitrogen, AM2694). Samples were immediately frozen and kept at −80 °C until further processing. Following a previously published protocol for parallel DNA and RNA sequencing from individual cells (Macaulay, I. C. et al., 2016), genomes and transcriptomes of lysed cells were separated Amplified cDNA was prepared on-bead according to the Smart-seq2 protocol. Libraries were prepared using Nextera XT (Illumina)