16S rRNA amplicon seq: SynCom Inoculant
DNA was purified from thawed cell pellets using the DNeasy PowerSoil Pro kit (Qiagen) and library preparations were performed according to the Illumina 16S Metagenomic Sequencing Library protocol (https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/16s/16s-metagenomic-library-prep-guide-15044223-b.pdf) for the V3 to V4 region of the 16S rRNA gene. The gene library was indexed using Nextera XT Index Kit Set A and B (v2, Illumina Inc., USA) and ;braries were supplied with 40% PhiX control.