GSM6692831: ZKSCAN1_ADAR1; Homo sapiens; ncRNA-Seq
Total RNA was isolated using the RNeasy Mini Kit (Qiagen, Hilden, Germany).  Circular RNA was enriched by removing rRNA (rRNA minus kit, Thermo Fisher) and digesting RNAs with RNAse R. The libraries were sequenced from both ends of the fragments for a total of 150nt from each end a NovaSeq SP flow-cell (Illumina). The fastq read files were generated and demultiplexed with the bcl2fastq v2.20 Conversion Software (Illumina, San Diego, CA). The quality of the demultiplexed fastq files was evaluated with the FastQC software, which generates reports with the quality scores, base composition, k-mer, GC and N contents, sequence duplication levels and overrepresented sequences. RNAseq of circular RNAs