GBS of Fusarium graminearum isolate KSU23453
Each sample's genomic DNA was digested with 2 restriction enzymes, PstI and MspI. After restriction enzyme inactivation, digested DNA was ligated to Y adapters and barcode adapters in a modification of the protocol given here: https://doi.org/10.1371/journal.pone.0032253. After ligation heat inactivation, libraries from multiple strains were pooled then amplified by PCR with the primers published in the previously referenced article.