GSM8087286: daf-2_control_egg_88h_rep 3; Caenorhabditis elegans; RNA-Seq
RNA was extracted using 300 uL Trizol reagent. Worms were vortexed continuously for 20 minutes at 4C and then went through one cycle of freeze-thaw to help release RNA. Following this, RNA was purified using the Direct-zol RNA MiniPrep kit (Zymo Research) per the manufacturer's instructions using on-column DNase I digestion to remove genomic DNA. Total RNAs were polyA enriched, and directional RNA-seq libraries were prepared using NEBNext Ultra II RNA library prep Kit.