GSM7843439: whole body, group, male, 9 dph, biol rep 1; Nothobranchius furzeri; RNA-Seq
Total RNA was isolated with TRIzol reagent (Thermo Fisher Scientific), and subjected to DNase treatment with a TURBO DNA-free kit　(Invitrogen #AM1907) according to the manufacturer's instructions. For whole juvenile fish and livers, the libraries were prepared using NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490), NEBNext Ultra II Directional RNA Library Prep Kit (E7760), NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (E6440). For ovaries and testes, the libraries were prepared using TruSeq stranded mRNA Library (Illumina, CA, USA).