Transcriptome sequencing of Lipaphis erysimi at nymph stage
Total RNA was extracted from ~50 morphs in each sample using RNeasy Mini Kit (Qiagen, Hilden, Germany) following manufacturer's instructions. Total extracted RNA was quantified using Qubit 4.0 fluorometer (Thermo Fisher Scientific, Waltham, MA, USA) and its integrity and quality were assessed using Tapestation 2200 (Agilent, CA, USA). Purified total RNA was used for transcriptome library preparation using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA). cDNA library was quantified using Qubit 4.0 fluorometer (Thermo Fisher Scientific, Waltham, MA, USA) and quality assessment was done by Tapestation 2200 (Agilent Technologies, Santa Clara, CA, USA). Transcriptome sequencing was performed on Illumina NovaSeq 6000 platform (Illumina, San Diego, CA, USA) with 151-base pair (bp) paired-end reads.