GSM7844453: TBP ChIP_Rep2_Sth1-AID/Sth1-KR+Aux+Met [TBP_cn-59]; Saccharomyces cerevisiae; ChIP-Seq
Sonicated extracts were used to ChIP for either Rpb3, Spt16, Spt6 or TBP antibodies. ChIPs samples were reverse-crosslinked, extracted with chloroform:IAA, then precipitated with ethanol. Libraries were constructed using NEBNext Ultra II kit and Biooscientific NEXTFLEX ChIP-Seq adapters. Protocol was slightly modified from NEBNext manufacturer instuctions.