Shotgun metagenomic sequencing of human gut metagenome
Microbial DNA was extracted using the QIAamp Fast DNA Stool Mini Kit and automated with the QIAcube system. Library preparation for metagenomic sequencing utilized the NEBNext Ultra DNA Library Prep Kit for samples below 200ng and the NEBNext Ultra II DNA Library Prep Kit for samples exceeding 200ng. MGI in China performed metagenomic shotgun sequencing on DNBSEQ-G400 platform.