GSM7663571: E7_embryo_male_WT_rep2; Mus musculus; RNA-Seq
RNA was isolated from untreated male and female E7.0 embryos for whole transcriptome sequencing (n = 5 females, 7 males from 7 litters). No more than two embryos from each sex were used per litter. Libraries were prepared using the SMARTer Ultra Low Input RNA and Nextera XT DNA kits by the UNC High Throughput Sequencing Facility. Samples were pooled prior to sequencing and run paired-end (2x100) on a NovaSeq 6000 instrument using the S4 XP workflow.