GSM7898922: kura_pool_rep1_hashing_GAGATTCC,replicate 1,CITE-Seq HTO; Homo sapiens; RNA-Seq
Cells were trypsinized and single-cell suspensions were counted and pooled for cell hashing method using Total-SeqA hashtag oligos HTO 1-10 (Biologend) according to manufacturers protocol. The cell pool was loaded for droplet-based scRNA-Seq using the 10X Genomics Chromium platform according to the manufacturer's protocol. Library preparation was performed using the 10X Genomics Chromium platform with the Chromium Single Cell 30 Library & Gel Bead Kit v3.1 following the manufacturer's instructions. For the hashtag oligos, 1μl of HTO PCR additive primer was added to the 10X cDNA amplification step, and the supernatant from the 0.6X cDNA cleanup was kept and processed according to the Cell Hashing protocol, with 14 PCR cycles and a 1.2X cleanup after the PCR. Libraries were diluted to 2 nM and 75bp paired-end sequencing was performed using the Illumina NextSeq 500