GSM6373904: PC12 cells,control group,rep 1; Rattus norvegicus; RNA-Seq
Total RNA was extracted from cells using TRIzol reagent according to the manufacturer's protocol. 1 μg of total RNA was subjected to library synthesis using the TruSeq V2 RNA-Seq kit.1 μg of total RNA was enriched for the Poly-A mRNA and reverse transcribed to double-stranded cDNA. The cDNA was end repaired, adenylated, and ligated with Illumina indexes prior to 12 cycles of PCR. Sequencing was performed using 12pM hybridization to a 2x100 paired end flow cell. RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.