RNA-seq of exported RNA barcodes in supernatant of cells after 10 cells were sorted into a well and cultured for 24 h, replicate well 5 of 8
RNA was extracted from supernatant of cell culture. RNA barcodes were reverse-transcribed and amplified by PCR. Resulting libraries were purified twice using magnetic beads. Sequencing was performed on the Illumina Miseq platform.