Illumina HiSeq 4000 paired end sequencing; Genotyping of C.elegans mutants - CRISPR/Cas9 of all GPCR and neuropeptide genes
Genotyping of C.elegans mutants - CRISPR/Cas9 of all GPCR and neuropeptide genes
Animals were grown on 9 cm plates until starvation. 2 plates of worms were collected and washed 3 times in M9 buffer. Genomic DNA was extracted using DNeasy Blood and Tissue kit (Qiagen, 69504). The DNA concentration was determined using Qubit (Thermo Fisher Scientific). 1 ug of genomic DNA in the elution buffer was used for library preparation using Nextera Flex.