single-cell RNA-Seq of homo sapiens: adult female high grade serous carcinoma tumor
Tumor chunks were cryopreserved in liquid nitrogen, then thawed and dissociated to single cells using the Miltenyi Human Tumor Dissociation Kit and the GentleMACS dissociator. Library preparation was performed using the 10x Genomics 3′ Gene Expression Library Prep v3 and libraries were sequenced on an Illumina NovaSeq instrument.