GSM7091725: U+L, +ORF1, +ORF2-Cas9; Arabidopsis thaliana; OTHER
Genomic DNA was isolated from frozen Arabidopsis seedlings. PCR amplicons were generated using the primers shown in the manuscript. Sequencing libraries were constructed from the PCR amplicons using the Nextera DNA Flex kit (Illumina) and Hackflex protocol (PMID: 35014949), as described in PMID: 34750500. After quality control, the library with pooled amplicons was sequenced SE300 on an Illumina MiSeq.