RNA-Seq of Acartia tonsa: adult
Specimen storage: After removing the seawater, around 500 Acartia tonsa adults were stored in the fridge  (4C) in RNAProtect (Qiagen) for one day.  RNA extraction: RNAProtect was removed and the specimens were ground using a sterilized micropestle, keeping the Eppendorf tube in liquid nitrogen. RNA was extracted using the RNeasy Protect Mini Kit (Qiagen). RNA evaluation: Once extracted, the quantity, quality and integrity of the RNA obtained was assessed with the Qubit RNA High sensitivity kit, the NanoDrop 1000 and the Agilent Bioanalyzer 2100, respectively. Library preparation: Equimolar amounts of RNA obtained from the untreated organisms and from those exposed to two different concentrations of neonicotinoid were mixed and 60 ng of this pool was used for the synthesis of the sequencing library that was prepared following the instructions of the cDNA-PCR sequencing kit (SQK-PCS109, Oxford Nanopore).