GSM7928695: 18_33260, oldie, RNA-seq; Homo sapiens; RNA-Seq
Suction blister roofs, each 7 mm in diameter, were obtained from the volar forearms by applying negative pressure at 180 mbar for 30 minutes, followed by 320 mbar until blister formation. The blister roofs were then carefully prepared using surgical scissors and tweezers. They were immediately snap frozen in liquid nitrogen and stored at -80°C until further use. Total RNA was extracted from the roofs of the suction blisters using the RNAeasy Fibrous Tissue Mini Kit (Qiagen, Inc.) according to the manufacturer's instructions. Libraries were prepared using TruSeq Library Prep Kit (Illumina) following manufacturer's instructions.