GSM8086549: Tnm+union-1; Mus musculus; RNA-Seq
Mouse 4T1 breast cancer cells were cultured in complete medium containing 90%DMEM, 10% fetal bovine serum (high quality) and 1% penicillin streptomycin at 37℃ in a 5% C02 incubator, and cell suspension with cell concentration of 1×10^7/ml was prepared for use. Thirty healthy BALB/c mice, aged 4 to 6 weeks and weighing 18 to 20 g, were randomly divided into 5 groups, 5 in each group. The ck group was injected with the same amount of normal saline, and the BALB/c mice in the other 4 groups were subcutly inoculated with 0.2ml cell suspension of corresponding concentration (the number of cells was about 200W) on the chest wall of 5-6 intercostocal and lateral abdominal walls. The four groups were named Tnm, Tnm+swim, Tnm+cur and Tnm+union. Each afternoon, the Tnm group and Tnm+swim group received 200ul solution intragastric administration, and the Tnm+cur and Tnm+union group received 200ul curcumin solution dissolved in corn oil (100mg/Kg) intragastric administration. Half an hour after gavage, Tnm+swim and Tnm+union groups were subjected to swimming exercise intervention for 30 minutes, and weight and tumor volume were weighed every two days. PE150 platform used forbasecalling. sequenced reads were trimmed for adaptorsequence,and masked forlow-complexity or low-qualitysequence,then mapped tomm9 whole genome using HISAT2 v2.0.5. We used HTSeq(0.9.1) statistics to compare the ReadCount values on each gene asthe original expression of thegene, and then used FPKM tostandardize the expression.Difference expression of genes was analyzed by DESeq(1.30.0) with screened