Safonov.MGR1.repl1_R1.fastq
DNA was isolated from grey microbial mat formed in a thermal fracture water stream seeping out of a rock using FastDNA Spin Kit for Soil (MP Biomedicals, USA) including mechanical cell lysis using a FastPrep-24 5G homogenizer (MP Biomedicals, USA). Amplicon libraries were prepared using primers for the V4 region of 16S rRNA gene. PCR was performed on a StepOne Plus Real-Time instrument (Thermo Fisher Scientific, USA).