GSM7717221: non_1; Homo sapiens; RNA-Seq
Total RNA was isolated from HUVECs using an RNeasy Mini Kit (Qiagen K.K., Tokyo, Japan). Full-length cDNA was prepared using a SMART-Seq HT Kit (Takara Bio, Japan) according to the manufacturer's instructions. According to the SMARTer kit instructions, an Illumina library was then prepared using a Nextera DNA Library Preparation Kit (Illumina).