GSM6454225: PSAT1_P4 bio rep2; Homo sapiens; RNA-Seq
Following treatment, cells were harvested by trypsinization and lysed in QIAzol Lysis Reagent. RNA was extracted using the miRNeasy Mini RNA Extraction kit (Qiagen, UK) as per manufacturer's instructions. Total RNA (100 ng) was used for library preparation using the Illumina TruSeq® Stranded Total kit, according to the manufacturer's guidelines. The libraries were sequenced by the Next Generation Sequencing Facility at the University of Leeds, using the NextSeq 500 (Illumina, California, USA) to generate single end 75 bp reads.