GSM8005170: HPCs, HG+NC lenti-virus, 24 h, rep2; Homo sapiens; RNA-Seq
Total RNA was extracted with Trizol (Invitrogen) The mRNA purified by Oligo(dT)-attached magnetic beads was fragmented into small pieces with fragment buffer at appropriate temperature. Then First-strand cDNA was generated using random hexamer-primed reverse transcription, followed by a second-strand cDNA synthesis and purified using AMPure XP Beads. The cDNA library was constructed through repair, PCR amplification, and purification. Qubit 2.0 and Agilent 2100 bioanalyzer were used for quantification.