GSM7766123: 839 cells,sgControl_1; Homo sapiens; ATAC-seq
Cells were harvest and washed in ice-cold PBS followed by centrifugation at 500 g for 5 minutes. Cells were resuspended in 50 ul cold lysis buffer (10 mM Tris-HCl pH 7.5, 3 mM MgCl2, 10 mM NaCl, 0.1% Igepal CA-630) and mixed end over end. After centrifugation at 500 x g for 10 minutes, cell pellet was resuspended in transposition reaction mix (25 μl TD-Buffer, 2.5 μl Tn5, 22.5 μl water) and incubated at 37 °C for 30 min with gentle mixing. Immediately following the transposition reaction, MinElute PCR Purification Kit (Qiagen) was used to purify transposed DNA. Finally, the PCR amplification was performed to amplify purified DNA and qPCR-based methods were used to quantify ATAC-seq libraries.