Total RNA sequencing of Leishmania braziliensis MHOM/BO/17/VP
Parasites were cultivated in the M199 medium (Sigma-Aldrich/ Merck, Darmstadt, Germany) supplemented with 2 g/ml Biopterin (Merck), 2 g/ml Hemin (Jena Bioscience GmbH, Jena, Germany), 25mM HEPES (VWR/ Avantor, Radnor, USA), 50 units/ml of penicillin, 50 g/ml of streptomycin (both from Biowest, Nuaill, France), and 10% Fetal Bovine Serum (Biosera, Cholet, France) at 23C as described previously. The total genomic DNA and RNA were isolated from 10 ml of trypanosomatid cultures with the DNeasy Blood & Tissue and RNeasy Mini kits (both from Qiagen, Hilden, Germany) according to the manufacturers instructions