GSM6630620: Veh_siScramble_Rep3 [RNA-seq]; Mus musculus; RNA-Seq
Pellets from isolated cardiac fibroblasts transfected with H1.0 or scrambled siRNAs and treated with TGF-β or vehicle were sent to the UCLA Technology Center for Genomics & Bioinformatics core for RNA isolation, library preparation and sequencing. Ribosomal RNA was removed using KAPA RNA HyperPrep kit (Cat.kk8561, Roche). Approximately 40 million paired-end reads per sample (2x150bp) were generated during sequencing. Paired-end library construction