Amplification-free CRISPR/Cas9-enriched DNA sequencing of AML, APL, or CML: cell lines, peripheral blood, bone marrow.
DNA was extracted from cell lines and primary specimens using PureGene (Qiagen), AllPrep DNA/RNA Kit (Qiagen), or Qiagen X-tractor with Reagent Pack DX. cRNA guides were designed to direct Cas9 to cut in the genomic proximity of each region studied. Enrichment of targeted regions was obtained using Oxford Nanopore Technologies'  Targeted, amplification-free DNA sequencing using CRISPR-Cas9 protocol (Library Strategy), and libraries were sequenced using Oxford Nanopore Technologies' MinION instrument (Instrument Model).