GSM6909082: 3b; Homo sapiens; RNA-Seq
Total RNA was isolated from ~20 mg of the pre- and post- biopsy samples using Trizol followed by spin-column for cleanup and DNAase treatment (Qiagen, Hilden, Germany). Total RNA was eluted in 50 µl of PCR grade water and concentration was quantified using a spectrophotometer (Nanodrop, Thermo Scientific, Waltham, MA) and integrity via 2100 Bioanalyzer (Agilent, Santa Clara, CA). Libraries were prepared from total RNA according to Illumina instructions. Stranded RNA Library Prep was performed on a Wafergen Bio-systems Apollo 324 Robot (Fremont, CA). Libraries were quantified by qPCR.