GSM7663604: E10.5 otocyst wild type replicate 1; Mus musculus; RNA-Seq
Otocysts were collected from E10.5 embryos in ice-cold PBS and RNALater (Ambion AM7021). RNA was isolated from pairs of otocysts from three embryos of each genotype (WT, Chd7Gt/+, Sox2CreER/+ and Chd7Gt/+;Sox2CreER/+) using the RNAqueous Micro Scale RNA Isolation kit according to the manufacturer's instructions (Ambion AM1931, Austin, TX). Sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina according to the manufacturer's instructions (NEB E7760, Ipswich, MA).