GSM7928674: ATAC_CAL27; Homo sapiens; ATAC-seq
Take out the cryovial and shake it in 37℃ water,Cells were centrifuged and it was counted with LUNA-FL.Live cells undergo a series of treatments and nucleus was collected. the nucleus pellet was resuspended in the Tn5 transposase reaction mix.It was incubated at 37℃ for 30 min. Equimolar Adapter1 and Adatper 2 were added after transposition.After the PCR reaction, libraries were purified with the AMPure beads.