GSM7748824: heart, vehicle, biological rep6; Rattus norvegicus; RNA-Seq
Collected and flash frozen hearts from 3-week-old male offspring were sent to Genome Quebec (Montreal, Quebec, Canada) for total Trizol-phenol RNA extraction. Quality of RNA was assessed using the RNA integrity number (RIN), with all samples having a RIN score ≥ 7.0. Libraries were generated from RNA as follows: mRNA enrichment was performed using Illumina Stranded mRNA Prep (Illumina); adapters and PCR primers were purchased from IDT; libraries were quantified using the KAPA Library Quantification Kits - Complete kit (Universal) (Kapa Biosystems).