RNA-Seq of HCT116 Lamin(B1&B2)-AID_48 hr Auxin_Rep2
Total RNA extraction was performed using the RNeasy Plus Mini Kit (Qiagen, #74134). RNA-Seq libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs, #E7760), according to the companys instruction. Library quality was measured using the Qubit 2.0 and Bioanalyzer. Bulk mRNA sequencing was conducted in The Genome Analysis and Technology Core in Charlottesville, VA at the University of Virginia. Paired-end reads were acquired using NextSeq 2000 (75bp) system on high throughput mode.