GSM6690515: 'Quanhong poplar'(QHP)，leaves 3; Populus deltoides; RNA-Seq
Extraction of the total RNA from leaf samples was performed using the EAZY spin Plus Plant RNA Kit. The contaminated DNA was removed from the extracted RNA using DNase I (TAKARA). The Dynabeads mRNA Purification Kit (Invitrogen) was used fot the total RNA to generate mRNA, which were furtehr fragmented and reverse transcribed into first strand cDNAs initiated by random hexamer priming.Then the double-stranded cDNAs (ds-cDNA) were generated by the NEBNext Ultra RNA Library Prep Kit (NEB), which were purified, end-repaired, and ligated to adaptors. Finally, the dscDNAs with adding adaptors were further fragmented and enriched by PCR to construct the final cDNA libraries for Illumina paired-end sequencing. The cDNA library was sequenced using the Illumina NovaSeq 600