GSM7401821: Arabidopsis, FLAG tag mutant RGA in sly1 dP [Frga11sdP-R1]; Arabidopsis thaliana; ATAC-seq
Nuclei purification using sucrose sedimentation for ATAC-seq libraries preparation ATAC-seq libraries were prepared using the protocol from Greenleaf, W. J lab and Kieber, J. J lab with slight modifications.200 mg of ground tissue was used. and 400 μL of chromatin and incubated at 4 ‎°C with rotation for 2 hr.