GSM6938000: 6-HPI-TR3-15_OZP1604_Inoculated; Pisum sativum; RNA-Seq
The total RNA from 100 mg of leaf sample was isolated using SpectrumTM Plant Total RNA kit (Sigma Aldrich, St. Louis, MO, USA) and Qiagen plant mini kit as per the manufacturer's procedure. The quality of RNA samples was assessed by loading on to the 1% agarose gel and the concentration was checked by Nanodrop 2000 spectrophotometer (Thermal co., USA). The total RNA extracted from each replicate was used to prepare transcriptome libraries using Agilent's Sure Select Strand Specific RNA Library Preparation Kit (Agilent Technologies) according to the manufacturer's protocol