GSM7575089: WT_treated_rep1; Bacillus subtilis; RNA-Seq
Cultures were harvested at OD ranging from 1.4 to 1.5 and immediately centrifuged at 10,000xg for 2 minutes using a Heraeus Multifuge X3R Centrifuge. The resulting pellet was then rapidly transferred to liquid nitrogen. RNA was extracted using the Spectrum Plant Total RNA kit (Sigma-Aldrich). Paired-end libraries were prepared with the NEBNext Ultra II RNA Library Prep Kit for Illumina® (New England Biolabs) according to the manufacturers' protocol.