GSM6631258: Mutant 3; Vibrio cholerae; RNA-Seq
The intracellular bacterial cells were recovered by lysing the amoeba with 1% Triton-X and total RNA was extracted using the RNeasy plus mini kit (Qiagen, Hilden, Germany). A combination of lysozyme and proteinase K was used to ensure complete lysis. RNeasy plus mini kit's gDNA shearer column as well as RNase free DNase (Qiagen, Hilden, Germany) on column DNA digest were used to ensure sufficient degradation of the contaminant DNA in the sample. RNA-seq libraries were prepared using Illumina TruSeq Stranded mRNA kit according to the manufacturer's protocol (Illumina, San Diego, CA, USA).