GSM6431317: TAM112 grains, 5 DAP, rep2; Triticum aestivum; RNA-Seq
The powdered grain tissues have been extracted with 100mM Tris pH 8.0, 150mM LiCl, 50nM EDTA, 1.5% sodium dodecyl sulfate (SDS), 1.5% 2-mercaptoethanol, and phenol/chl/IAA. The supernatant is further extracted with Trizol, precipitated with isoproanol, then purified using Qiagen® RNeasy Plant Kit. RNA library was constructed using NEXTFLEX Rapid Directional RNA-Seq Kit 2.0, following the manufacturer's protocol.