GSM6821375: NFO190_159-VeroE6; Chlorocebus sabaeus; RNA-Seq
For CLEAR-CLIP, cells were washed twice with ice cold PBS, UV254 cross-linked or not, scrapped, collected, pelleted by centrifugation at 500 g for 5 min at 4C and snap frozen, before to be gently lysed and processed further as described in PMID: 26602609 with minor changes. For RNA-Seq, cells were washed twice with ice cold PBS, dissolved in ice-cold reagent and total RNA extracted using a RNA Clean & Concentrator-25 kit (Zymo Research Z1018) strictly following manufacturer instrcutions and adding a DNAse I step. For CLEAR-CLIP, library was constructed following a custom-made protocol (see PMID: 26602609) and minor changes. For RNA-Seq, library was constructed strictly following the TruSeq Stranded Total RNA protocol from Illumina and using TruSeq RNA CD index plate (96 indexes, 96 samples).