ITS for metagenome of Malus domestica: apple fruit
Microbial genomic DNA samples were extracted using the FastDNA SPIN Kit for Soil (MP Biomedicals, Solon, OH, United States) following the instructions of the manufacturer. Extracted DNA was used as the template for amplicon PCR reactions that amplified the fungal internal transcribed spacer (ITS) 1 region. The ITS1 region was amplified using the ITS1F/ITS2 primer set (Ghannoum et al., 2010, White et al., 2013).