GSM8229000: well2, Feature barcode; Homo sapiens; OTHER
Cell suspensions following harvesting of bone marrow and spleen tissues from individual mice were cryopreserved. NSG mice had been injected with human T cells. Upon thawing, samples underwent dead cell removal (Miltenyi MACS kit) and human CD45 enrichment (Stem Cell Technologies). 5 spleen or bone marrow samples were pooled together (one sample per treatment group), and 32,000-40,000 cells were loaded in one channel of a Chromium Chip K (10x Genomics) and profiled using the Chromium Next GEM Single Cell 5' Kit v2 (10x Genomics). Full-length paired α/β TCR libraries were obtained using the Chromium Single Cell V(D)J Enrichment Human T Cell (10x Genomics). cDNA, gene expression, and TCR library quality was assessed using the DNA High Sensitivity Bioanalyzer Chip (Agilent). Gene expression or TCR libraries were pooled and sequenced on a NovaSeq 6000 sequencer (Illumina).