GSM7899090: Bulk E13 Parotid gland epithelium, replicate 4; Mus musculus; RNA-Seq
Submandibular salivary glands at embryonic day (E) 13 and parotid glands at E13 were harvested. Four independent biological replicates were collected. The TRIzol RNA isolation method was used (Invitrogen). RNA quantity and integrity were assessed using a 2100 Bioanalyzer (Agilent Technologies). All samples for sequencing had RNA integrity (RIN) values > 8.7. Total RNA per sample was reverse transcribed, and full-length cDNA was generated using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio USA). Libraries for Illumina sequencing were prepared using the Nextera XT kit (Illumina), pooled, and analyzed on four lanes of a NextSeq 500 (Illumina).