GSM7765209: TM rep9; Sus scrofa; RNA-Seq
Total RNA from samples in each group (n=10) was extracted using the Trizol method (Invitrogen Life Technologies, Gaithersburg, MD), according to the manufacturer's instructions. The RNA concentration, quality and integrity were determined with NanoDrop spectrophotometer (Thermo Scientific). The transcriptome libraries of each group (n=10) were constructed for sequencing using the KAPA-Stranded RNA-Seq Library Prep Kit (Illumina).