PacBio WGS of Bacillus coagulans strain JBI-YZ6.3
High molecular weight genomic DNA of strain JBI-YZ6.3 was purified using Qiagen genomic tip kit. The genomic DNA purity and integrity were checked using Nanodrop and Agilent TapeStation, respectively. The PacBio SMRTbell library (~20 kb) was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA). The library was bound to polymerase using the Sequel Binding Kit 3.0 (PacBio) and loaded onto PacBio Sequel using Sequel Sequencing Kit 3.0.