GSM7928416: mm202C_CRISPR_Epi_7d_VTP_REP3_S11; Mus musculus; OTHER
DNA was extracted from cell pellets at the indicated timepoint using the phenol:chloroform extraction method. We used a modified two-step PCR version of the protocol published by Doench et al. 2016. After an initial “enrichment” PCR, whereby the integrated sgRNA cassettes were amplified from gDNA, a second PCR was performed to append Illumina sequencing adapters on the 5′- and 3′-ends of the amplicon, as well as a random nucleotide stagger and unique demultiplexing barcode on the 5′-end.