Illumina NovaSeq 6000 paired end sequencing; Inflammatory signals from fatty bone marrow support DNMT3a-driven clonal hematopoiesis (Dnmt3A KI heterozygote subset)
Inflammatory signals from fatty bone marrow support DNMT3a-driven clonal hematopoiesis (Dnmt3A KI heterozygote subset)
Bone marrow cells were collected from the femora and tibia bones. BM was flushed with a 1cc (23G) into IMDM (Iscove's Modified Dulbecco's Medium). The cells were enriched by a c-kit CD117 and sorted by FACS. RNA extraction was performed using the 10x V3 Chromium technology. Libraries were prepared by the 10X genomics V3 3â€™ kit.