FGR+PE mild
RNA was isolated from the placental biopsies using TRIreagent (Thermo Fisher, Waltham, WA) and processed using the RNeasy mini kit (Qiagen, Germantown, MD), RNA quality was assessed using an Agilent bioanalyzer (Agilent Technologies, Santa Clara, CA) and an Agilent HS Total RNA 15nt kit (Agilent, #DNF-472T33) on an Advanced Analytical 5300 Fragment Analyzer RNA concentration was quantified with a Qubit HS RNA assay kit (Invitrogen, Waltham, MA, #Q32855) on a Qubit 4 fluorometer (Invitrogen, #Q33238). Total RNA-seq libraries were generated with the Illumina Stranded Total RNA Prep kit with Ribo-Zero Plus (Illumina, San-Diego, CA, #20040529) according to the manufacturer's instructions.