GSM7400992: ATAC, E10.5 EC, rep 2; Mus musculus; ATAC-seq
1500 cells were sorted directly into 100% FCS, then nuclei were isolated by resuspending in  lysis buffer (10 mM Tris-HCl pH7.5; 10 mM NaC; 3 mM MgCl2; 10% IGEPAL CA-630; 0.1% TritoxX-100), and incubating 10 min on ice. Protocol adapted from Buenrostro et al. 2015. Nuclei were resuspended in 10 uL tagmentation mix (0.35 uL Tn5 transposase; 5 uL 2X tagmentation buffer; 4.65 uL nuclease free water) (Illumina Nextera FC-121-1030), then incubated 37C for 30 min. Tagmentation reaction was quenched using 1.1 uL 500 mM EDTA, and 10 uL 50 mM MgCl2 was added to the sample. The library was amplified using 13 PCR cycles  on the thermocycler, then purified using a MinElute kit (Qiagen).