GSM6454653: 540-8mo-mutant_S38; Mus musculus; RNA-Seq
Following immunoprecipitation, ribosomal-bound RNA was isolated using the Nucleospin miRNA isolation kit (Clontech) allowing purification of large and small RNA species. Bulk RNA from miR-142-null and heterozygote mice as well as rTg4510 mice was generated by fresh dissection of the frontal cortex (rTg4510) or prefrontal cortex (miR-142-null), homogenization in lysis buffer and RNA isolation using the Nucleospin miRNA isolation kit. RNA quality was measured using an Aligent Bioanalyzer generating RINs between 6.1-9.5 for all samples. RNA-sequencing was carried out using either the NuGEN RNA amplification with RiboZero treatment with 50 bp paired end reads (M dataset) or the Illumina TruSeq Stranded RNA library kit with RiboZero Gold treatment with 75 bp paired end reads (T and P datasets).