GSM7749220: IFN_GEX; Mus musculus; RNA-Seq
Immune cells were prepared by peritoneal cavity lavage (peritoneal cavity) or mechanical disruption (spleen), followed by staining with flourescent antibodies, hashing with Totalseq-A antibodies against MHCI and CD45, and flow sorting macrophages, B cells and CD4+ T cells into DMEM/2%FCS Libraries were prepared according to the 10x 3' (samples 1-8) and 10x3'v3 (samples 9-15) GEX protocol. Hash libraries were prepared according to the New York Genome Center's cell hashing/CITEseq protocol.