GSM7749234: VDJ Istoype_03; Mus musculus; OTHER
MCA205 tumors were collected, enzymatically dissociated and T cells were sorted, counted and resuspended in PBS + 0.5% human albumin  in a 1:1 CD4+:CD8+ ratio. A total of 10000 cells were loaded to the 10x Chip K for emulsion and barcoding. Libraries were constructed according to the manufacturer's protocol (Next GEM Single Cell 5' Reagent Kits v2 + mouse TCR Amplification Kit, Dual Index) scRNA-seq + TCRseq