GSM7900503: RIPseq_ctrl plus DPO replicate i; Vibrio cholerae; RIP-Seq
Cells equivalent to 50 OD600 units were collected and cells were lysed using glass beads. RNA-Co-immunoprecipitation using anti-FLAG-antibody following protein G sepharose, Solution was washed and RNA fraction was isolated with phenol-chloroform-isoamylalcohol. RNA was treated with TurboDNase to remove DNA contaminations. doi.org/10.1073/pnas.1920753117 The rRNA depleted RNAs samples were fragmented using magnesium fragmentation module (NEB). cDNA was synthesized by using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB)  according to the manufacturer's instructions. cDNA libraries were column-based purified and cleaned left-sided using the Agencourt AMPure XP kit (Beckman Coulter Genomics). The quality and quantity of cDNA libraries was measured by Bioanalyzer and Qubit, respectively.