GSM7766120: Small-intestine LP ILC2s, donors, scRNAseq; Mus musculus; RNA-Seq
Leukocytes were isolated from kidneys by digestion and CD45 enrichment using CD45-microbeads (Miltenyi) and from SILP by digestion and percoll gradient. The ILC2s were sorted as CD45+Lin-CD127+IL-13fm+ Single-cell libraries of sorted cells were generated with the 10X Genomics Chromium Single Cell 5'v1.1 reagents kit according to the manufacturer's protocol.