16S amplicon of soil metagenome from apple orchard
DNA was extracted from 0.5 g of soil using E.Z.N.A. SoilDNA Kit and further purified using Mag-Bind TotalPure NGS Omega kit.341F and 785R primers were used toamplify V3V4 16S rRNA gene region using Q5 Hot Start High-Fidelity polymerase. Sequencing (PE: 2 x 300 nt) was done with Illumina v3 kit on MiSeq.