RNA-Seq of Homo sapiens: 46XY unstimulated CD4+ T cells
Cells were collected following 24 hours of in vitro culture and RNA was extracted using the Zymo  Quick RNA Microprep kit. 2uL of a 1 to 1000 dilution of ERCC Spike-Ins were added per 100ng of RNA.   Libraries were prepared using the IDT xGen RNA Library Preparation kit according to manufacturer's instructions.