GSM7717044: 10 PCW snRNAseq; Homo sapiens; RNA-Seq
Samples were microdissected by peeling the epicardial/subepicardial layers from the ventricle and from the peritruncal region of the Aorta and of the Outflow tract and resuspended in cold DMEM + 0.02% BSA + 10% FBS. Epicardial preparation was first resuspended in WB (Wash Buffer) and then moved to a freshly prepare lysis buffer (LB). Chromium Single Cell 3' Library & Gel Bead Kit v3 and Single Cell Multiome ATAC + Gene Expression Kit following the manufacturer's instructions