GSM7662520: iBMDM_Untreated_1; Mus musculus; RNA-Seq
A Direct-zol RNA Extraction Kit (Zymo Research, Cat no. R2072) was used to extract RNA according to the manufacturer's protocol. Libraries were prepared using an Illumina Stranded mRNA Library Prep kit (Illumina, Cat no. 20040534) with IDT for Illumina RNA Unique Dual Index adapters following the manufacturer's recommendations, except that half-volume reactions were performed. Generated libraries were quantified and assessed for quality using a combination of Qubit™ dsDNA HS (ThermoFischer Scientific, Cat no. Q32851) and Agilent 4200 TapeStation HS DNA1000 assays (Agilent, Cat no. 5067-5584). The libraries were pooled in equimolar amounts, and the pooled library was quantified using an Invitrogen Collibri Quantification qPCR kit (Invitrogen, Cat no. A38524100).