GSM6820923: Fig.3, HeLa ICS screen, input, lib 1; Homo sapiens; OTHER
RNA from iPSC-CMs: fixed cells were pelleted after ICS sorting, reverse crosslinked at 56C for 1h in the presence of proteinase K, and RNA was extracted using the TRIzol-chloroform-isopropanol protocol; 1-10ng of RNA were used for library preparation. DNA from HeLa: cells were pelleted and genomic DNA was extracted using NEB Monarch genomic DNA purification kit, including the RNase treatment and elution in 50 µl elution buffer; 100-500 ng of DNA was used for library preparation. iPSC-CMs: RNA libraries were generated using SMARTer RNA-Seq Kit v3 - pico (Takara Bio) according to manufacturer's instructions; HeLa ICS screen: see detailed methods in Schraivogel et al., 2022. iPSC-CMs: RNAseq; HeLa ICS screen: Amplicon seq.