GSM6935679: H1hESC_G3KO_72h_Rep2; Homo sapiens; ATAC-seq
Cells were dissociated using Gentle Cell Dissociation Buffer (STEMCELL Technologies). 50,000 cells per ATAC reaction were processed according to the OMNI-ATAC protocol (Corces et al., 2017), using Illumina Tagment DNA Enzyme and Buffer Kit (Illumina, #20034197) for 30 minutes. Transposed DNA was purified using the Zymo DNA Clean and Concentrator Kit (Zymo Research, D4014). Libraries were amplified using NEBNext Ultra8482 II Q5 MasterMix (New England Biolabs; M0544). Amplified DNA was purified using KAPA Pure Beads (KAPA Biosystems; KK8000).