scRNA-seq of mus musculus: Gli1-postive muscle cells
Gli1+ cells were enriched by FACS sorting into wash medium and centrifuged at 500 g at 4C for 5 min. Collected cells were resuspended in PBS + 0.5% BSA, then counted by hemocytometer and controlled for quality. If > 98% of visible objects were verified to be single cells, the sorted cells passed quality control, and were processed with a Chromium Controller (10 x Genomics), captured and libraried with the Chromium Single Cell 3 Library Kit v2 (10 x Genomics).