GSM8182868: K group replicate 2; Homo sapiens; OTHER
5×105 cells was collected in the 1.5 ml tube with 1mL culture medium(Gibco,11965092),and formaldehyde solution was added to achieve a final concertration of 1%. Then, the tube was placed in a Rotator for 15minuters at RT. After cross-linking, the remaining formaldehyde was sequestered by adding 27.9 μL of 2 M glycine (Sigma, G7126) and continued to rotate for 10 minutes at RT. Then, the restriction enzyme HaeIII was used for digestion. Biotin-labeled bridge linkers was used to ligate DNA fragments. Purify labled DNA fragments with Dynabeads(Invitrogen, 11047 ).Quantify the recovered DNA.The purified DNA is used directly for cluster generation and sequencing analysis using the Illumina novaseq6000 following the manufacturer's protocols. BL-HiC