GSM7567486: Tumor derived from KO+NM1 01; Mus musculus; RNA-Seq
RNA from both tumors and cells were isolated by TRIreagent according to manufacturers' protocol. For transcriptional profiling of tumor tissues, NM1 KO tumor and KO+NM1 tumor were divided to 4 equal pieces and each piece separately homogenized in RNAzol reagent by using the Bead Ruptor 96 Homogenizer (Omni International). Total RNA from 3 replicates of NM1 KO tumor and 4 replicates of KO+NM1 tumor was extracted according to the manufacturer's protocol.The RNA-Seq library was prepared by using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB) and sequenced with the NextSeq 500/550 sequencing platform (performed at the NYUAD Sequencing Center).