GSM7091153: WGBS_Ana_HF_Setdb1_Het_rep1; Mus musculus; Bisulfite-Seq
FACS-sorted cells were subjected to genomic DNA extraction with Qiagen DNeasy Blood & Tissue Kit (Cat# 69504) following the manufacturer's instructions. The genomic DNA spiked with lambda DNA were fragmented to 200-400bp. The DNA fragments were bisulfite treated to generate single strand DNA. During the bisulfite conversion, unmethylated cytosine were converted into uracil, while methylated cytosine staid unchanged. Methylation sequencing adapters were ligated, followed by double strand DNA synthesis, size selection and PCR amplification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection.