GSM7766085: CT_D; Mus musculus; ncRNA-Seq
RNA of FACS-sorted β-cells  was extracted with the miRNeasy kit (Qiagen) and EU-tagged RNA was biotinylated and pulled down using the Click-iT Nascent RNA Capture kit (Thermo Fisher C10365). For each condition, the same amount of RNA was incubated with streptavidin-coated beads and, after washing, the biotinylated RNA attached to the beads was eluted using the following Elution Solution: 95% Formamide, 10 mM EDTA in H2O. Eluted RNA was purified again with the miRNeasy kit and used for library preparation. Libraries were prepared with QIAseq miRNA Library Kit (Qiagen) following manufacter's protocol.