GSM7091158: CD4-Cre ICOS KO; Mus musculus; RNA-Seq
10 mL of collagenase solution containing 300 U/mL collagenase type II (Worthington Biochemical) and 10 mg/mL DNAse I (MilliporeSigma) was added to each lung and digested for 50 minutes at 37 °C with 300 rpm horizontal shaking. Lung digests were then filtered through 40 μM cell strainers, and red blood cells were depleted. Cells were filtered once more through 70 μM cell strainers Libraries were generated at the Institut de Recherches Cliniques de Montréal Molecular Biology and Functional Genomics Core facility using the following components from 10x Genomics: Chromium Next GEM Chip G Single Cell kit, Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead kit v3.1, Chromium i7 Multiplex kit. Sequencing was performed by Genome Québec using a NovaSeq 6000 (Illumina).