GSM7899342: KOPTK1 cells, shDHX15+MYC-3; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol reagent (Thermo Fisher Scientific) and subjected to quality control for RNA integrity by Agilent Bioanalyzer 2100 system (Agilent Technologies). Total RNA was further purified by RNAClean XP Kit (Beckman Coulter) and RNase-Free DNase Set (QIAGEN). Poly(A)+ RNA libraries were constructed using VAHTS Universal V6 RNA-seq Library Prep Kit (Vazyme) and sequenced as 150 bp paired-end reads by Illumina NovaSeq 6000 (Annoroad Gene Technology Corporation).