GSM7717370: HepG2 m6A-IP rep2; Homo sapiens; OTHER
Total RNA was isolated from HeLa cells with TRIzol reagent. Poly(A)+RNA was further enriched from total RNA by using Dynabeads™ mRNA Purification Kit (Invitrogen).m6A-IP was performed using EpiMark N6-Methyladenosine Enrichment Kit following the manufacturer's protocols. Libraries were constructed with SMARTer Stranded Total RNA-Seq Kit v2 (Takara) according to the manufacturer's protocols. All libraries were sequenced on Illumina Nova Seq with single-end 100 bp read length.