GSM8138090: Normoxia_KO_5; Mus musculus; RNA-Seq
Isolatedbrain endothelial cells were harvested from animals and homogenized in TRIzol solution for RNAseq analysis according to the manufacturer's instructions. Following isolation, RNA quality was assessed using an Agilent Tapestation 4200. Only samples with with an RNA Integrity Number (RIN) greater than 8.0 were used to generate RNA sequencing libraries using the Illumina Stranded mRNA Prep according to the manufacturer's insturctions.