GSM7899250: Healthy, iPS-VSMC, oxLDL, pos, 1; Homo sapiens; RNA-Seq
Total RNA from untreated or oxLDL-treated healthy- and WS-iPS-EC was isolated using an RNeasy Micro Kit (QIAGEN) ~10 ng total RNA was used as input for cDNA conversion using a SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (cat. #634890, ClontVECh, Takara). cDNA was fragmented using an S220 Focused-ultrasonicator (Covaris). The cDNA library was then amplified using a NEBNext® Ultra™ DNA Library Prep Kit for Illumina (cat. #E7370L, New England Biolabs).