GSM8228762: FLASH_45_FGF4LY_Rep5_S26; Mus musculus; RNA-Seq
Extraction and library preparation were performed in one reaction (see below) Library preparation from single ICMs was  performed according to FLASH-seq protocol (V. Hahaut et al., “Fast and highly sensitive full-length single-cell RNA sequencing using FLASH-seq.” Nat Biotechnol 40, 1447-1451; 2022) with modifications from Festuccia et al (Nr5a2 is essential for morula development. Biorxiv, 2023.01.16.524255) including 3' phosphorylation of the template switching primer and addition of 7 cycles of linear enrichment PCR for UMI reads.