GSM6449773: small preB_1; Mus musculus; RNA-Seq
proB, largepreB, smallpreB (BM):  Erythroid, myeloid, T cells, NK cells and mature B lineage (Lin) cells were depleted from bone marrow cell suspensions, and Lin(–)cKitloCD19+CD43+ pro-B cells, Lin(–)CD19+CD43+BP1+ large preB cells, and Lin(–)CD19+CD43–CD25+ small preB cells were sorted by FACSAria (BD Biosciences).    ETP, DN2, DN3, preDP (Thymus):  Erythroid, myeloid, gdT, CD8+CD4+DP, CD8+T, CD4+T, NK and B lineage (Lin) cells were depleted from thymocyte cell suspensions, and Lin(–)CD44+cKit+CD25– ETP, Lin(–)CD44+cKit+CD25+ DN2, Lin(–)CD44–CD25+ DN3, and Lin(–)CD44–CD25– preDP cells were sorted by FACSAria (BD Biosciences).      smallDP, CD8+T, CD4+T (Thymus):  Erythroid, myeloid, NK and B lineage (Lin) cells were depleted from thymocyte cell suspensions, and CD8+CD4+ small DP, CD8+T, and CD4+T were sorted by FACSAria (BD Biosciences).    RNA was isolated using Trizol reagent and Direct-zol RNA prep kit (Zymo Research). Illumina TruSeq RNA Sample Prep Kit (Illumina) or NEB Next Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB) were used for the construction of sequencing libraries.