GSM7766565: WT, PF, S16; Mus musculus; RNA-Seq
Cells were lysed in TRIzol reagent. After centrifuging, the supernatant (aqueous phase) was used to extract total RNA using the RNeasy kit (Qiagen, 74106) according to its protocols. RNA libraries for RNA-seq were prepared using the NEBNext® Ultra™ II Directional RNA Library kit following manufacturer's protocols.