GSM7931887: engrafted_KAPP_rep2_snRNA; Mus musculus; RNA-Seq
Tumor-bearing C57BL/6J mice were euthanized by CO2 exposure. Brains were rapidly dissected in ice-cold dissociation medium (DM) containing: 20 mM glucose, 81.8 mM Na2SO4, 30 mM K2SO4, 5.8 mM MgCl2, 250 µM CaCl2, 1 mM HEPES, 160 µM NaOH, 0.8 mM kynurenic acid, 50 µM D-APV, 100 U/mL penicillin, 100 µg/mL streptomycin, 5 µg/ml plasmocin and 100 µg/ml primocin. Coronal sections were cut using a brain matrix and GFP+ (tumor) and GFP- regions (stroma) were microdissected under an epifluorescence stereomicroscope (Leica M205, Leica Biosystems). Microdissected tissue was then enzymatically digested into a single-cell suspension using the Papain Dissociation System according to the manufacturer's instructions (Worthington Biochemicals). The samples were sequenced using the 10X Chromium single-cell 3' protocol.