GSM7400740: E13.5 FL SE-hi coculture; Mus musculus; RNA-Seq
FACS-isolated primary cells or cultured cells harvested by trypsinization (for FL-EC) or pipetting (for co-cultured hematopoietic cells) were washed with PBS/0.04% ultrapure BSA, and resuspended in PBS/BSA for loading into the 10X Genomics Chromium Single Cell B Chip, and processed in the Chromium single cell controller. The 10X Genomics Version 3.1 single cell 3' kit was used to prepare single cell mRNA libraries for freshly isolated primary cells or FL-EC, while the 10X Genomics Version 3.1 with Feature Barcoding technology for Cell Surface Protein Sequencing was used for co-cultured cells. Both kits were used according to manufacturer protocols with the Index Kit T Set A. Sequencing was performed for pooled libraries from each sample on an Illumina NextSeq 500 using the 75 cycle, high output kit.