scATAC of primary tumor
Nuclei were spun down and resuspended in 100 ul of lysis buffer for 4 min on ice. Nuclei were washed with 200-400 ul wash buffer and counted with trypan blue staining, then resuspended with sufficient nuclei buffer to achieve a concentration of 2000 nuclei/ul. To perform tagmentation, 5 ul of nuclei suspension was added to 7 ul of ATAC buffer (10X Genomics) and 3 ul of Tn5 and incubated at 37°C for 1 h. Nuclei capture and library prep was then performed according to the 10X Genomics scATAC-seq protocol.