RNA-seq of MDA-MB-231 xenograft tumors
To evaluate the effects of flotillin-1 palmitoylation, MDA-MB-231 cells were engineered to express flotillin-1 WT or palmitoylation defective (C34A). WT flotillin-1 cells were labelled with a GFP reporter and C34A cells with a dTomato reporter. GFP-WT and dTomato-C34A cells were implanted bilaterally into mice in their #4 mammary fat  and allowed to form tumors for 8 weeks. Tumors were digested into single cell supsensions and flow sorted based on GFP or dTomato reporters. Gene expression was then compared between GFP-flotillin-1-WT and dTomato-flotillin-1-C34A MDA-MB-231 cells from xenograft  tumors.