GSM7900731: scalp dermis, White Leghorn, E9, ATAC-Seq rep 2; Gallus gallus; ATAC-seq
ATAC resuspension buffer (ARB) was comprised of 3mM MgCl2, 10mM NaCl, and 10mM Tris-HCl pH=7.4. 50,000 dissociated cells of indicated tissues were lysed in 50 μl of cold ATAC lysis buffer (0.1% NP-40, 0.1% Tween-20, and 0.01% digitonin freshly added in ARB) for 5 min. 1 ml of ARB (with 0.1% Tween-20) was added and mixed well by inverting the tube. 50,000 nuclei were harvested for the Tn5 transposase reaction. The tagmented DNA was purified using a DNA Clean & Concentrator-5 kit (Zymo Research, Cat. D4014). Nextera DNA Library Preparation Kit was used for ATAC-seq library preparation. The final libraries were purified utilizing 1X AMPure XP beads (Beckman, A63880).