Mol16S/Cop16S amplicon sequencing of plankton tow: April 2018 P4 vertical KA Ohio rep2
Single index used for independently-amplified reactions: Mol16S primers (Klymus et al., 2017) (35 cycles, 58 degC annealing), Cop16S primers (Clarke et al., 2017) (35 cycles, 45 degC annealing). Indexing with 8 cycle PCR; MiSeq PE 2x301 bp at the Molecular and Cellular Imaging Facility (Ohio State University).