GSM8085985: ileum, WT CME(+), rep1; Mus musculus; RNA-Seq
Total RNAs were harvested using TRIzol (Invitrogen) and Rneasy Mini Kit (Qiagen) with Dnase I treatment (Qiagen). 100 ng of total RNAs was used for the construction of sequencing libraries. cDNA libraries were prepared using QuantSeq 3' mRNA-seq V2 Library Prep Kit with UDI (Lexogen)