GSM7900862: HEC1B DMSO control replicate 4 (batch 3); Homo sapiens; RNA-Seq
Cell lines were trypsinized and organoids scraped. Cells were pelleted and stored at -80°C until RNA was prepared. RNA was prepared using Qiagen's RNEasy Mini kit (Qiagen Cat #74104) with on-column DNASE digest (Qiagen Cat. #79254) according to the manufacturer's protocol. RNA was snap frozen and sent to GENEWIZ (South Plainfield, NJ) for Standard RNA sequencing. RNA libraries for RNA-seq were prepared by GENEWIZ.