NextSeq 550 paired end sequencing; A transcriptome analysis study on mRNA and long non-coding RNAs in patients with Parkinson's disease
A transcriptome analysis study on mRNA and long non-coding RNAs in patients with Parkinson's disease
PBMCs separation was performed using Ficoll-Paque (Ficoll Plaque PLUS – GE Healthcare Life Sciences, Piscataway, USA) RNA was extracted using TRIzol reagent (TRIzol Reagent, Invitrogen Life Technologies, Carlsbad, CA, USA), according to the manufacturer's instructions Indexed libraries were prepared from 1 µg/ea purified RNA with TruSeq Stranded mRNA (Illumina) Library Prep Kit according to the manufacturer's instructions. Libraries were quantified using TapeStation 4200 (Agilent Technologies) and pooled such that each index-tagged sample was present in equimolar amounts, with a final concentration of the pooled samples of 2 nM.