GSM6821330: F23_23 DRN; Oryctolagus cuniculus; RNA-Seq
After collection, lungs were immediately placed in RNA Later (Sigma Aldrich, USA) and stored at -20°C. Samples were homogenized in QIAzol® Lysis Reagent. Total RNA was extracted with the miRNeasy Mini Kit protocol (QIAGEN, Germany), using an automated method (QIAcube: QIAGEN, Germany), adapted to include DNase I treatment. Libraries for massive parallel RNA sequencing (RNA-seq) were prepared with the QuantSeq FWD (Lexogen) kit and sequenced with the use of an Illumina NextSeq500 platform, which generated at least 20 million reads/sample (75 bp Single End).