GSM7664322: Injury NMDA 36hr, snRNA-seq (to be replaced); Danio rerio; RNA-Seq
Zebrafish retinas (4-6 retinas from 4-6 fish) were collected at different timepoints after NMDA and LD treatments, and flash-frozen in dry ice for ~15min before being transferred to a -800 C freezer for storage. Nuclei were extracted from frozen retinal tissues according to 10xMultiome ATAC + Gene Expression (GEX) protocol (CGOOO338). Briefly, frozen retinal tissues were lysed in ice-cold 500ml of 0.1X Lysis buffer using a pestle and incubated on ice for 6 min totally. Nuclei were centrifuged, washed 3 times and resuspended in 10xMultiome nuclei buffer at a concentration of ~3000-5000 nuclei/ml. Nuclei (~15k) then were loaded onto 10x Genomic Chromium Controller, with a target number of ~10K nuclei per sample. RNA and ATAC libraries were prepared according to the 10xMultiome ATAC + Gene Expression protocol and subjected for Illumina NovaSeq sequencing at ~500 million reads per library