GSM8089077: XEN cells, Ctcf-degron, DMSO treated, RNA, rep2; Mus musculus; RNA-Seq
Cells were lysed in lysis buffer (0.2% (vol/vol) Triton X-100 and 2 U/μl RNase inhibitor). The polyadenylated mRNAs were captured by the oligodT primers, mRNAs were subjected to the Smart-seq2 protocol as described previously (Picelli et al., 2014).