GSM7663759: DIFF MOCK; Homo sapiens; RNA-Seq
Freshly collected cells were resuspended in ice-cold Hibernate E medium supplemented with 0.25% BSA at a cell concentration of 1,100 cells/µl for single-cell RNAseq Indexed libraries were prepared from 10 µl of cDNA using Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (10x Genomics). Library and cDNA quality were evaluated using the Agilent 2100 Bioanalyzer Instrument and quantified using the ViiA 7 Real-Time PCR system. Constructed libraries were sequenced on an Illumina NovaSeq 6000 using paired-end reads to achieve a final sequencing depth of approximately 80,000 reads per cell.