Ion Torrent S5 XL sequencing; RNA-sequencing of porcine heart after myocardial infarction with or without human umbilical cord-derived MSC (hUCM-MSC) delivery
RNA-sequencing of porcine heart after myocardial infarction with or without human umbilical cord-derived MSC (hUCM-MSC) delivery
A total of 400 ng of extracted RNA from the remote LV myocardium was used for RNA sequencing after ribosomal and mitochondrial RNA depletion. RNA extracted using the protocol as described in RNEasy Plus Mini Kit (Qiagen) Ion Total RNAseq kit: reverse transcription, cDNA amplification, barcoded adapters hybridization and ligation. Quality control in agilent 2100 Bioanalyzer