GSM7766079: small-intestine LP ILC2s, S1; Mus musculus; RNA-Seq
Leukocytes were isolated from kidneys by digestion, percoll gradient, erythrocyte lysis and CD45 enrichment using CD45-microbeads (Miltenyi); from lungs by digestion, percoll gradient, and erythrocyte lysis; and from SILP by digestion and percoll gradient. After sorting of kidneys and lungs ILC2s as CD45+Lin−CD127+CD25+Sca 1+ and of ILC2s from SILP as CD45+Lin−CD127+CD25+KLRG1+, total RNA was extracted using using the RNeasy Plus Micro Kit (QIAGEN) according to the manufacturer's instructions 250-300 bp cDNA insert library, 6G raw data/sample on average