GSM7898908: kuramochi_C_drug_end; Homo sapiens; OTHER
Cells were trypsinized and single-cell suspensions were counted and pellets were frozen for genomic DNA extraction according to Birsoy et al. Cell, 2015. An initial pool of cells was harvested for genomic DNA extraction as in Birsoy et al. 2015. Remaining cells were cultured for 10 population doublings either off (C, T10, T320) and on olaparib treatment (C in 1 μl and T320 in 40 μl), after which cells were harvested for genomic DNA extraction. sgRNA inserts were PCR-amplified, gel purified, quantified with NEBNext Library Quantification Kit (#E7630S) and sequenced on a Illumina NextSeq 500, generating between 20-40 million paired reads for each library.