WGS of evolved Bacillus subtilis : P1bC21
Paired-end (evolution alone) or single-end (evolution with Pseudomonas) libraries were prepared using the NEBNext Ultra II DNA Library Prep with Sample Purification Beads. Fragment reads were generated on an Illumina NextSeq sequencer using TG NextSeq 500/550 High Output Kit v2 (300 cycles) (RNOmique Platform, UdeS). The methodology for processing the reads drew inspiration from the GenPipes framework. We first implemented a quality-based trim of the reads using fastp 0.21.0, setting the parameters to --cut_right --cut_window_size 4 --cut_mean_quality 30 --length_required 30.