DNA seq of ancient sheep metagenome from Mentese Hoyuk, not UDG treated
Library was constructed using 5l starting DNA, purified from the EDTA wash rather the bone pellet itself, following the dsDNA Meyer and Kircher protocol (Meyer & Kircher, 2010) without uracil DNA glycosylase treatment, to capture native damage patterns. Following dual indexed-PCR amplification for 12 cycles, library was shotgun sequenced on NovaSeq 6000 platform (TrinSeq, Dublin)