GSM6594879: SAM24353298; Mus musculus; RNA-Seq
Lumbar DRGs L4, L5, L6  (6 DRGs) from one mouse were used per biological replicate. We chopped the DRGs and enzymatically digested them using a combination of collagenase and trypsin with gentle shaking.  DRG cells were then dissociated by gentle trituration. The cell suspensions were purified by myelin elimination using magnetic anti-myelin beads and suspended in HibernateA containing 10% inactivated horse serum. Viable cells were counted using Calcein-AM labeling. Cells were washed 3x and suspended in HibernateA medium containing 10% inactivated horse serum, 0.2 U/uL RNase inhibitor for single cell capture and transcript library generation. Libraries were generated using the 3' gene expression library kit v3 (10X Genomics) following manufacturer's protocol.