GSM8229701: leaf tissue, Fe deprivation, biol rep 1; Populus trichocarpa; RNA-Seq
Total RNAs for Fe deprivation samples were isolated using the Spectrum Plant Total RNA Kit with on-column Dnase treatment (Sigma); Total RNAs of PtrbHLH011 OE samples were isolated using the RNeasy Plant Mini Kit (Qiagen) and treated with on-column DNase (Qiagen) to remove genomic DNA contamination. Illumina RNASeq w/PolyA Selection for Ctrl and Fe deprivation samples; DNBSEQ Eukaryotic Strand-specific mRNA library for Empty vector and PtrbHLH011 OE samples