GSM7717036: Ctrl2; Homo sapiens; ncRNA-Seq
The circRNA-seq data was generated using a standard RNA extraction protocol. Briefly, total RNA was isolated from the sample using TRIzol (Invitrogen) , according to the manufacturer's instructions. The extracted RNA was treated with DNase to remove any genomic DNA contamination, followed by purification steps to obtain high-quality RNA suitable for downstream applications.The circRNA-seq library was prepared using a modified version of the standard circRNA-seq library construction protocol. The library construction process involved several steps. First, use the kit to deplete rRNA, then deplete linear RNA to enrich circRNA. Then， the circRNA was subjected to a reverse transcription reaction using random primers to convert the RNA into cDNA. The enriched circRNA was fragmented and ligated with adapters. Subsequently, PCR amplification was performed to amplify the library and generate sufficient material for sequencing.The library strategy used for circRNA-seq was strand-specific RNA-seq. This strategy allows the identification and characterization of circular RNAs by preserving the information regarding their originating strands. It provides insights into the differential expression, alternative splicing, and other features of circular RNAs in the sample. RNA libraries were prepared for sequening using standard Illumina protocols