GSM7664260: FUSP525L Mutant (5th differentiation); Homo sapiens; RNA-Seq
We performed RTqPCR, using progeny from 1 well of 6-well-plate for RNA extraction using the Quick-RNA Microprep Kit (Zymo research) according to the manufacturer's instructions with addition of DNAseI from the On-Column DNase I Digestion kit (Sigma-Aldrich). Library preparation was performed with the LexoGen Quant Seq. Kit. Libraries were sequenced on an Illumina HiSeq4000 sequencing system.