GSM7663419: control healty sample - rep 9; Homo sapiens; miRNA-Seq
Total RNA extraction was performed using TRIzol reagent (TRIzol Reagent, Invitrogen Life Technologies, Carlsbad, CA, USA), according to the manufacturer's instructions. Total RNA was used for sequencing library preparation using NEXTFLEX Small RNA-Seq Kit v3 (Perki-nElmer) according to the manufacturer's instructions. Libraries were quantified using the TapeStation 4200 (Agilent Technologies) and Qubit fluorometer (Invitrogen Co.), then pooled such that each index-tagged sample was present in equimolar amounts. The pooled samples were subject to cluster generation and sequencing using an Illumina NextSeq 550 Dx System (Illumina) in a 1x75 single-end format.