GSM7766166: Cortical neurons, shControl, shScrambled, Day 11, Rep3; Mus musculus; ATAC-seq
Cells were treated with DnaseI to eliminate DNA contamination. Tn5 transposomes were applied to lysed chromatin. Transposed chromatin was purified using Zymo DNA Clean & Concentrator-5 columns. Transposed DNA libraries were prepared using NEBNext Ultra II Q5 Master Mix and Illumina-compatible unique 5′-i5 and 3′-i7 barcode sequences.