WGS of ASFV isolate LIS1957/PIR
Virus DNA was extracted from concentrated DNAase treated virus obtained from infected bone marrow macrophage cultures exhibiting >90% cytopathic effect. The resulting DNA was extracted using the MagAttract high molecular weight DNA kit (Qiagen) and then amplified using REPLI-g (Qiagen). The presence of ASFV DNA was confirmed by qPCR (King et al., 2005) and only DNA with > 10E6 genome copies were used to generate libraries. 2-4 g DNA was barcoded using Nanopore Native barcoding genomic DNA kit and Genome ligation kit. Samples were then run on a MinION (MIN-101b) using a 9.4.1. flow cell for 16 hours and then the flow cell was flushed using flow cell wash kit and the run for a further 24 hour period.