bac_fun__leaf/needle under ES2 at 200d
Genomic DNA were extracted from degrading leaves/needles in forest ecosystem. For bacteria, the primer pair 515F (5-GTGCCAGCMGCCGCGGTAA-3) and 806R (5-GGACTACHVGGGTWTCTAAT-3); and for fungi fITS7 (5-GTGARTCATCGAATCTTTG-3) and ITS4 (5-TCCTCCGCTTATTGATATGC-3) were used.