Anthracnose resistance in highbush blueberry: gene expression in berries following inoculation
RNA was extracted using the MagMaxTM plant RNA Isolation Kit (Applied Biosystems/ThermoFisher Scientific) from ~50 mg of frozen ground tissue according to the manufacturer's recommended protocol for use with the KingFisherTM Flex Magnetic Particle Processor 96DW (ThermoFisher Scientific). RNase-free PVP40 (2% w/v) was added to the lysis buffer according to the manufacturer's recommendation to deal with polyphenol- and polysaccharide-rich samples. On-Column DNase digestion with the RNase-free DNase Set was used to remove DNA in the RNA samples (Qiagen, Valencia, CA, USA). RNAseq libraries were prepared using the mRNA HyperPrep Kit for Illumina Platforms and the Dual-Indexed Adapter Kit for Illumina platforms and then sequenced using 150-bp paired-end reads on an Illumina HiSeq4000 system