GSM6431123: TXR1 knockout,replicate 2, OK-seq, single-indexed; Tetrahymena thermophila; OTHER
Genomic DNA was extracted by a previously described NDS extraction method followed by ethanol precipitation, dissolved in H2O, treated with RNAse A 5µl (10mg/ml) ,98oC heat denatured and chilled on ice. Okazaki fragment were isolated and biotinylated. Sequencing adaptor were ligated and Okazaki fragment capture using Strep-Magnetic beads. Libraries were ampliﬁed in 15 cycles of PCR using bead suspension as a template, Phusion polymerase (NEB) and the following primers: Amplify F2, Amplify R2. PCR products were gel-purified to select for fragments 150–400 bp using gel elution kit (Qiagen).