GSM7898876: pc9_pool_C_to_T1.2_P1.2,scRNA-Seq; Homo sapiens; RNA-Seq
Cells were trypsinized and single-cell suspensions were counted and pooled for cell tagging method using the 10X Genomics CellPlex kit Set A according to manufacturers protocol. The cell pool was loaded for droplet-based scRNA-Seq using the 10X Genomics Chromium platform according to the manufacturer's protocol. Library preparation was performed using the 10X Genomics Chromium platform with the Chromium Single Cell Library & Gel Bead Kit v3.1 following the manufacturer's instructions. For sample pooling, cell suspensions from each corresponding sample were labeled using the 10X Genomics 3' CellPlex kit Set A according to the manufacturer's instructions. Cells were pooled in equal amounts and loaded in the 10X Genomics Chromium Controller. For A375 experiment, the correspondence between samples and CMO tags are: C: CMO301; T003: CMO302; T006: CMO303; T0125: CMO304; T0250: CMO305; T0500: CMO306; T1: CMO307; T2: CMO308 and T4: CMO309). For the PC9 experiment, the correspondence between samples and CMO tags are: C: CMO301; T0008: CMO302; T0016: CMO303; T0032: CMO304; T0075: CMO305; T0150: CMO306; T0300: CMO307; T0600: CMO308; T1.2: CMO309; P1.2: CMO310