GSM7092295: Memory_KO_H3K4me3_003; Mus musculus; ChIP-Seq
NK cells were purified and ixed with 1% formaldehyde, washed with TBSE buffer, and resuspended in 1% SDS lysis buffer. Cells were sonicated and immunoprecipitation was performed with anti-H3K4me3 polyclonal Ab and anti-H3K27ac polyclonal Ab, and bound to Dynabeads Protein A. Beads were reverse-crosslinked and DNA was recovered. ChIP DNA was processed into DNA sequencing library using NEBNext Ultra II DNA Library Prep Kit for Illumina.