GSM8278867: ChIP-seq_D2_Blimp-1_MA5_rep1; Homo sapiens; ChIP-Seq
For ChIP-seq, genomic DNA was purified with QIAGEN MinElute Reaction Cleanup Kit. ChIP DNA were end-repaired using an End-It DNA End-Repair kit (Epicentre), universal adaptors were then ligated. The libraries were amplified using indexed primers with the PCR condition: 98°C, 10”; 67°C, 30”; 72°C, 30” for 16 cycles. The fragments between 180bp to 350bp were isolated on E-gel and subjected to sequencing on Illumina HiSeq2500.