Insectary bug fasting positive to T. cruzi
Digestive tracts of adult insectary and wild Triatomas pallidipennis were used. Digestive tracts were dissected under specific conditions in an RNAase-free environment. RNA extraction was standardized with a modified RNeasy minikit protocol. RNA quantification was performed with QIAExpert (spectrophotometry). RNA quality assessment was performed with 0.8% agarose gel electrophoresis. Samples were stored at -70C, the original tube (35-37uL) and a 3uL aliquot. Sequencing was performed with NextSeq 550 (2 x150 bp).