GSM8138998: MOLM-13 shCTRL H3K27ac rep1; Homo sapiens; OTHER
Fesh cells were bound to activated concanavalin A-conjugated paramagnetic beads and incubated overnight with the desired antibody. The next day they were incubated with pAG-MNase to cleave the chromatin. Proteins were removed and chromatin was precipitated with phenol-chloroform. Library construction was performed using the Kapa HyperPrep kit and Illumina's TruSeq adapter system. The amplified library was size-selected to remove leftovers of primers.