GSM8229920: prefrontal cortex, Saline 3; Rattus norvegicus; RNA-Seq
RNA Isolation was completed using the Direct-Zol RNA kits (Zymo Research Corp., Irvine, CA, USA) following the manufacturer's protocol Poly(A) RNA sequencing library was prepared following Illumina's TruSeq-stranded-mRNA sample preparation protocol. RNA integrity was checked with Agilent Technologies 2100 Bioanalyzer. Poly(A) tail-containing mRNAs were purified using oligo-(dT) magnetic beads with two rounds of purification. After purification, poly(A) RNA was fragmented using divalent cation buffer in elevated temperature