GSM7900253: JG06_patient7_distal; Homo sapiens; RNA-Seq
Human ILC3s (CD45+CD3-CD4-CD8-CD19-CD94-CD14-CD123-FcεRIa-CD34-CD11c-CRTH2-CD127+CD117+) were sort-purified from surgical-resection samples from the colon of patients with Ulcerative Colitis or Crohn's Disease. Sorted cells were used to prepare RNA sequencing libraries by the Epigenomics Core at Weill Cornell Medicine, using the Clontech SMARTer Ultra Low Input RNA Kit V4 (Clontech Laboratories). Sequencing was performed on an Illumina HiSeq 4000, yielding 50-bp single-end reads.