GSM7662525: wild-type Col-0, 4d, shoot, -P, +N [WT_E_J]; Arabidopsis thaliana; RNA-Seq
Samples of root or shoot material was stored in -80 °C. For homogenization, the samples were ground with mortar and pistil in liquid nitrogen. A maximum of 100 mg material was used for RNA extraction. RNA was extracted with TrizolTM (ThermoFisher Scientific, Waltham, USA) and chloroform according to the manufacturers protocol. The RNA isolation was followed by an additional cleaning step to remove access salts originating from the fungus tissue. For that, the sample was mixed with 3 M sodium acetate [1/10 (v/v) in RNAse-free water, pH= 5.2] and 600 µL of ice-cold 100 % ethanol and incubated at -20 °C for at least 1 hr. After centrifugation and 2 cleaning steps with 80 % ethanol the sample was resuspended in RNAse-free water. Library construction was done by Novogene Genomics Service (Cambridge, UK).