SMRT-seq of whole genome amplified DNA for Marchantia polymorpha Mpdn4mt1-1 sperm
Native WT and Mpdn4mt1-1 mutant sperm libraries for SMRT-seq were prepared using the SMRTbell Express Prep kit (v2.0; Pacific Biosciences, Menlo Park, CA, USA) following the low DNA input workflow as described (Kingan et al., 2019). Whole genome amplified libraries were also constructed, utilizing the SMRTbell gDNA Sample Amplification Kit (Pacific Biosciences, Menlo Park, CA, USA). Libraries were sequenced under CCS running mode (one Sequel II SMRT Cell for each native library and one Sequel II SMRT Cell for the amplified libraries) via DNA Link (Seoul, Korea).