Hi-C genome sequencing of Sesamia inferens
The sample processing for S. inferens in preparation involved a series of steps. Firstly, the cells were treated with polyformaldehyde to crosslink and fix the DNA conformation. After cell lysis, the crosslinked DNA was subjected to restriction enzyme treatment to generate sticky ends. The DNA ends were repaired and simultaneously labeled with biotin using an end repair kit. Subsequently, DNA fragments were ligated using DNA ligase enzyme. The crosslinked state of DNA was reversed by protein digestion, and the DNA was purified. The purified DNA was randomly sheared into fragments of 300-500 bp in length. Finally, the DNA fragments labeled with biotin were captured using streptavidin-coated magnetic beads, and a second-generation library was constructed for sequencing.