GSM7717283: Wild type CD8 T cells isolated from the lymph nodes and treated with Tc17 polarizing cytokines and antibodies; Mus musculus; RNA-Seq
Mesenteric lymph nodes were crushed to prepare single cell suspension using 0.70µm cell strainer and then CD8+ T cells were isolated using affininty beads from Miltenyi. The cells were cultured in RPMI media. The libraries were prepared using 10X Genomics Chromium Chromium Next GEM Single Cell 3' Reagent Kit v3.1 (Dual Index) according to the manufacturer's instructions.