GSM8087019: Mouse6_LowerTracheaNE_BioRep3; Mus musculus; RNA-Seq
Cells were sorted directed into Takara Smart Seq lysis buffer and then flash frozen. See Seeholzer and Julius 2024 for further details. We performed cDNA synthesis with Smart-Seq v4 kit according to the protocol recommendatioons (Takara Bio, 634890). cDNA library was generated by using Nextera XT DNA Library Preparation Kit (Illumina, FC-131-1024).  See Seeholzer and Julius 2024 for further details.