GSM8275134: Hippocampus, High Fat High Sugar Time Restricted Feeding, ad libitum, ORM1h, zt3, replicate 4; Mus musculus; RNA-Seq
RNA was harvested using pS6-TRAP protocol. 50ng of TRAP purified mRNA was used for the construction of sequencing libraries. TRAP-mRNA libraries for RNA-seq were prepared using Illumina Stranded mRNA Prep (Illumina, 20040534) and IDT for Illumina RNA UD Indexes Set B and Set C (Illumina, 20040554 and 20040555) following manufactured's protocols