GSM7900745: CD4+ T cells treated with MAPK13-IN-1; Mus musculus; RNA-Seq
Total RNA was extracted from purified CD4+ T cells using TRIzol (Invitrogen). For stranded RNA sequencing library preparation, 2 μg of total RNA was utilized with a Stranded mRNA Library Prep Kit from DR08502 (Bioyigene) following the manufacturer's instructions. Library products corresponding to 200-500 bp were enriched, quantified, and sequenced on the DNBSEQ-T7 platform.