GSM7663074: YBX1K2A-3; Mus musculus; RNA-Seq
RNA was extracted from renal tissue using RNeasy Mini Kit (QIAGEN, Germany).RNA integrity and quality were checked using an Agilent Technologies 2100 Bioanalyzer (Agilent Technologies; Waldbronn, Germany). The RNA sequencing library was generated from 500ng total RNA using Dynabeads® mRNA DIRECT™ Micro Purification Kit (Thermo Fisher) for mRNA purification. The ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicentre) was used to prepare the RNA sequencing library according to the manufacturer's protocols.