GSM7662897: Control_10_Rectum; Homo sapiens; RNA-Seq
Single cell libraries were prepared according to the protocol for 10X Genomics for Single Cell V(D)J + 5' Gene Expression. Approximately 20,000 sorted CD45+ cells were loaded and partitioned into Gel Bead In-Emulsions (GEM). scRNA libraries were sequenced on a HiSeq4000 (Illumina). The B and T cell receptor libraries were amplified according to the manufacturer's protocol and sequenced on a NovaSeqS4 (Illumina).