GSM7766686: Inner ear organoids,scRNA-seq,D20; Mus musculus; RNA-Seq
Whole inner ear organoids from day 16 to 21 of the Atoh1-nGFP ESC line were dissected, dissociated and flow sorted based on GFP expression. Cells were then loaded on 10X Genomics chip for scRNA-seq library preparation following the manufacturer's instructions. RNA libraries were prepared using standard 10X genomics protocols