GSM6593609: UK4_Calu3_DeltaNSP12_24h_3; Homo sapiens; RNA-Seq
Total RNA was extracted from 10^6 cells using the Qiagen RNeasy Kit after inactivating the virus post infection. The chosen method of rRNA removal was rRNA depletion for mRNA and long noncoding species. RNA was primed with random hexamers for first-strand cDNA synthesis and then second-strand cDNA synthesis. All samples were sequenced on HiSeq 4000.