Illumina NovaSeq 6000 paired end sequencing of SAMD00631497
Hi-C was performed as previously described (Lieberman-Aiden et al. 2009). Briefly, the extracted muscle of Gin rin showa was cross-linked with formaldehyde. Chromatin was digested with a restriction enzyme Hind III, biotinylated with Biotin-14-CTP, and then ligated with T4 DNA ligase. DNA was purified and sheared with Covaris . Biotinylated DNA interactions were pulled down with Dynabeads MyOne Streptavin C1 Beads (Life Technologies) and ligated with indexed Illumina Truseq adapters. Amplified libraries were sequenced with Illumina NovaSeq  X Plus platform.