GSM8278857: hiPSC-MNs_3; Homo sapiens; RNA-Seq
Differentiated human motor neurons were dissociated into a single-cell suspension using Accutase (Innovative Cell Technologies). Dissociated cells were resuspended in DMEM/F-12 medium (Thermo Fisher Scientific) containing 0.04%-BSA at a concentration of 700 cells/μL (Target cell recovery: 3,000 cells per channel). Subsequently, the suspended cells were loaded onto a Chromium Single Cell 3' Chip (10x Genomics) and processed through the Chromium Controller to generate single-cell gel beads in emulsion (GEMs) scRNA-seq libraries were prepared with the Chromium Single Cell 3' Library & Gel Bead Kit v.2 (10x Genomics) according to the manufacturer's protocol.