GSM7662488: CG replicate 1 48 hours; Homo sapiens; ATAC-seq
The transposition reaction was conducted while the cells were inside the hydrogels at 37C for 1 hour. The samples were then crushed and homogenized and the supernatant was collected and processed for library prep. Omni-ATAC-seq libraries were prepared using Nextera DNA CD Indexes (24 Indexes, 24 Samples) - Catalog # 20018707 and 20034197 ILMN Tag DNA Enzyme & Buffer Small Kit. Libraries were amplified using 5 cycles for amplification. Post-amplication libraries were size selected at 150-500 bp in length using SPRIselect beads. The libraries were validated using Agilent D5000 high sensitivity DNA kit in the Tapestation (Agilent).