GSM6594568: 3.glp-1; Caenorhabditis elegans; RNA-Seq
Reverse transcription was performed using SuperScript II reverse transcriptase (Invitrogen). cDNA was further PCR-amplified with KAPA HiFi HotStart ReadyMix (KAPA Biosystems) for 18 cycles. Uncontaminated samples were subjected to automated single-cell RNA-Seq library construction based on a Bravo robot station (Cui et al., 2019).