GSM7899398: ST2-U3C,interferon alpha,36h,rep1; Homo sapiens; RNA-Seq
Total RNA was extracted using the GeneMATRIX Universal RNA Purification Kit. 500 ng of purified total RNA was then reverse-transcribed using ThermoFisher Scientific reagents RNA libraries were prepared in three biological repeats from 1μg of total RNA using NEBNext® Ultra™ or Ultra™ II RNA Library Prep Kit for Illumina® (New England Biolabs, NEB) together with NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) and NEBNext® Multiplex Oligos for Illumina® (NEB), according to the manufacturer's protocol