GSM7663412: Mtb, Ab-SigA, ChIP-seq, replicate 2; Mycobacterium tuberculosis; ChIP-Seq
Mtb cells were treated with 1% formaldehyde for 30 min and the crosslinking was quenched by adding glycine to a final concentration of 250 mM for another 30 min. After bead-beating, the supernatant was treated with MNase for 15 min in a 37 °C incubator. The sequencing libraries for immunoprecipitated DNA and input control were prepared using the NEBNext Ultra II DNA Library Prep Kit. Libraries were amplified 11 cycles on the thermocycler.