GSM8307349: BAL_HTO; Mus musculus; RNA-Seq
Bronchoalveolar lavages were sampled using two intratracheal injections of 1 ml of PBS supplemented with 2% FCS. Cells were recovered after 5 min centrifugation at 400 g. After cell count and viability check, cell hashtag (HTO) staining (Biolegend, San Diego, CA, USA) was used following the cell-hashing protocol (TotalSeq™-A Antibodies and Cell Hashing with 10X Single Cell 3' Reagent Kit v3 3.1 Protocol). Library was performed according to the manufacturer's instructions (single-cell 3' v3.1 protocol, 10x Genomics).