Multiplexed Single Cell gene expression profile of barcoded SUM159PT cells
Multiplexed Single Cell gene expression profile of barcoded SUM159PT cells
Single cell suspensions (scRNA seq) were obtained according to manufacturer's instructions (10X Genomics). MULTI-seq samples were labelled with a sample-specific Barcode LMO-Anchor (as in Mc Ginnis CS et al.; 10.1038/s41592-019-0433-8 ) before loading on the Chromium Controller. scRNA-Seq Library preparation was performed according to the manufacturer's instructions (single cell 3' v2.1 or v3 protocol, Multiome 10x Genomics). MULTI-seq library were prepared following the MULTI-seq protocol, as in Mc Ginnis CS et al. DOI 10.1038/s41592-019-0433-8