GSM6431420: MCF10A_RNA_seq, time point 72h, TGF-beta1 treated, replication 2; Homo sapiens; RNA-Seq
Total RNA was isolated using the PeqGOLD total RNA kit (#732–2871, Peqlab/VWR Life Science, Bruchsal, Germany) Sequencing libraries were prepared using the TruSeq Stranded RNA Sample Prep Kit (Illumina) and paired-end sequenced (2x100 bp) on an HiSeq 4000 sequencing device (Illumina) at 50 million reads per sample.