Metagenome of fertile bulk soil from Chenopodium quinoa
For each plant, carefully dug up, soil farther than 1.5cm from the roots was collected and considered as bulk soil. Then, carefully we removed the soil 5mm away from the roots with brush and tweezers. Soil adjacent to the root segments, at 15mm from the root surface, was shaken off and defined as rhizosphere soil .The DNA was extracted from 250 mg of soil sample with three repetitions, using the PowerSoil extraction kit (MO Bio labs, Carlsbad, CA, USA), and according to the procedure manual described by the manufacturer.Once the quality and quantity of the metagenomic DNA has been verified, they are sent to the Laboratory of the Foundation for the Promotion of Health and Biomedical Research of the Valencian Community (Fisabio), Spain, for amplification and sequencing on the Illumina-Miseq platform. The ITS region  was amplified using the primer set ITS3_KYO2 (5GATGAAGAACGYAGYRAA3) and ITS4_KYO3 (5CTBTTVCCKCTTCACTCG3) for fungi.