Donor17,V2
Cryostat sections were lysed in a sucrose lysis buffer, filtered                 through a 70 m-pore cell strainer and nuclei purified by ultracentrifugation. Nuclei                 were labeled with anti-NEUN antibody, anti-OLIG2 antibody and Sytox blue.                 Single-nucleus cDNA libraries were constructed from sorted SytoxposNeuNnegOlig2neg                 fraction using the Chromium Single Cell 3 Reagents Kit V3 following the                 manufacturers instructions (10x Genomics).