GSM6824859: Mouse3_WT; feces metagenome; OTHER
Microbial genomic DNA were extracted with a QIAamp DNA Stool Mini Kit (Qiagen, 51504) following the manufacturer's instructions and eluted into 200 uL ddH2O. DNA was concentrated with a Quick-DNA Microprep Kit (Zymo, D3020). PCR for V3 and V4 hypervariable regions using barcoded primers. Sequencing adaptor were added to purified PCR product under manufacturers' guide. DNA library