GSM7662969: human oocytes, 100 µg/mL AfrLEA2_3; Homo sapiens; RNA-Seq
According to the experimental requirements, a control group (0M), 100 µg/mL AfrLEA2 group, 100 µg/mL AfrLEA3m group and fresh group were set up with 3 replicates in each group, and a total of 12 human oocytes were collected from the corresponding condition groups. After washing each oocyte twice in PBS, a volume of less than 5 µl of cells and fluid was aspirated and quickly transferred under a microscope to a clean 200 µl PCR (RNase-free) tube containing lysate, and each sample was placed on dry ice and frozen immediately after the addition of lysate for the next sample. The SMART-Seq v4 Ultra Low Input RNA Kit (Clontech, Japan) was used to prepare the low-input library following the procedures below: 1)Preparing cDNA library; 2)cDNA Purification and size selection (150-300bp); 3)Preparing sequencing ready library (add i5/i7adaptor using PCR method); 4)Library quality control (assess the size and concentration of the library using Agilent 2100 Bioanalyzer ); Library sequencing performed on Illumina platforms using a 2x150bp paired-end sequencing protocol