GSM7979340: Gabbr1_KO; Mus musculus; RNA-Seq
Single-cell suspensions with 1×105 cells/mL in concentration in PBS were prepared. Single-cell suspensions were then loaded onto microfluidic devices. ScRNA-seq libraries were constructed according to Singleron GEXSCOPE® protocol by GEXSCOPE® Single-Cell RNA Library Kit (Singleron Biotechnologies) . Individual libraries were diluted to 4nM and pooled for sequencing.