WGS of Mycobacterium tuberculosis variant bovis:isolation from bovine lung
The DNA samples that passed the electrophoresis test were randomly broken into fragments of approximately 350 bp in length using a Covaris ultrasonic crusher. The processed DNA fragments were then subjected to end repair, A-tail addition, sequencing junction addition, purification and PCR amplification using the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, USA).