GSM6631329: Matrigel high density beads derived EVs_biol rep 1; Homo sapiens; miRNA-Seq
Total RNA was extracted using the miRNeasy Serum/Plasma kit (cat. no. 77064; Qiagen, Inc.) according to the manufacturer's protocol. Extracted EV RNA was used to prepare miRNA next-generation sequencing (NGS) libraries with the QIAseq miRNA Library kit (cat. no. 331505; Qiagen, Inc.). 1) 3' end adaptor ligation: Ligate the 5-adenylated and 3-blocked adaptor to the 3' end of the small RNA fragment. 2) Add Unique molecular identifiers (UMI) labeled Primer. 3) 5' end adaptor ligation. 4) First strand synthesis with Unique molecular identifiers (UMI) labeled Primer. 5) Second strand synthesis. 6) Fragment selection. 7) Library quantitative and pooling cyclization. 8) Library QC. 9) Sequencing on DNBSEQ.