GSM8086270: Donor2, Ctrl & IL35, D2, multiplexing catpure; Homo sapiens; RNA-Seq
For each collection day, NK treated with or without IL-35 were barcoded following Cell Multiplexing Oligos (CMOs) labeling protocol (CellPlex Kit) developed by 10X Genomics. NK were independently incubated with specific CMOs, washed 3 times in PBS + 1% BSA and pooled together. The number of live cells was loaded on a 10X G chip to obtain an expected cell recovery population of 11,000 cells per channel and run on the Chromium iX system (10X Genomics) according to manufacturer's instructions. Gene Expression and Cell Multiplexing single-cell RNA-seq libraries were generated with the Chromium Single Cell 3′ v.3.1 kit with feature barcoding technology (10X Genomics). Gene Expression and Cell Multiplexing libraries were sequenced on the NovaSeq 6000 platform (Illumina) to obtain around 50,000 reads and 5,000 reads per cell respectively.