GSM8275339: MT_Sed_r29; Rattus norvegicus; RNA-Seq
Whole tissues (SM & LV) were mechanically disrupted with 2 cycles at 4m/s for 10 s (MP Biomedical FastPrep) in a cryotube with with a 5 mm stainless steel bead, and homogenised in TRI-reagent. SM & LV total RNA was extracted using spin-columns with on-column DNase-I digest as per the manufacturer's instructions (Zymo DirectZol RNA Miniprep kit #R2050, Zymo Research). Purified isolated mitochondria (MT) samples were homogenised in 5 volumes of TRI-reagent, then RNA was extracted using spin-columns with on-column DNase-I digest as per the manufacturer's instructions (Zymo Direct-Zol Micro Prep kit #R2060, Zymo Research). Skeletal muscle tissue total RNA (50 ng) and isolated mitochondria total RNA (10 ng) was converted to cDNA libraries (Zymo-SeqRiboFreeTotal RNA kit #R300, Zymo Research). Left ventricle tissue total RNA (100 ng) was converted to cDNA libraries (Illumina TruSeq Stranded Total RNA Ribo-Zero H/M/R)