full-length transcriptome of Danio rerio: 1k-cell
The RNA of each sample was reversely transcribed into cDNA using a SMARTer PCR cDNA Synthesis Kit. Prime STAR GXL DNA Polymerase Kit was used to PCR amplify the full-length cDNA. The SMARTbell libraries were constructed with the SMARTbell template prep kit. Conduct damage repair and end repair. The stem-loop sequencing adaptors were linked to both ends of the DNA fragment and used to remove the failed fragment using exonuclease.