GSM7979321: patient55; Homo sapiens; RNA-Seq
RNA was isolated from CD14+ monocytes using the Nucleospin RNA II kit with on-column genomic DNA digestion according to the manufacturer's protocol (Takara, Mountain View, CA, USA) cDNA was generated using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio), and the final RNA-seq library was generated using the Nextera XT kit (Illumina). RNA-seq libraries were sequenced on the Illumina NovaSeq 6000 with a sequencing depth of 30M PE100 reads per sample.