Whole genome Illumina NovaSeq sequencing of Chamaecrista mimosoides sample CHA6 using TruSeq library preparation.
High molecular weight genomic DNA was extracted from Chamaecrista sample CHA6 using a standard cetyltrimethylammonium bromide (CTAB) protocol with 1% polyvinylpyrrolidone (PVP). Nanodrop quantification indicated a DNA concentration of 786 ng/l. The sample was diluted 15:50 in nuclease-free water and quantified by Qubit at 181 ng/l. The Illumina library was constructed using the Truseq DNA nano library prep and sequenced on the Illumina NovaSeq 6000 plaform.