GSM7900554: wildtype myoblasts, differentiating (D2), Input 3; Mus musculus; ChIP-Seq
Cells were fixed in 1% formaldehyde, followed by formaldehyde quenching, washing and cell lysis. Lysed cells were sonicated using the Bioruptor PICO sonicator (Diagenode). Libraries were prepared by the Next Generation Sequencing facility at the Vienna Biocenter Core Facilities, Vienna, Austria, using the NEBNext® UltraTM II DNA Library Prep Kit for Illumina and unique NEB adaptors. Libraries were validated using the Agilent High Sensitivity DNA Kit.