GSM7402002: PH03016; lung cells, day 21, treatment; Mus musculus; RNA-Seq
RNA was isolated from lung tissue using Maxwell® RSC simplyRNA Tissue Kit (Promega, AS1340) Sequencing libraries from total RNA of the samples were prepared at the Core Facility Genomics, Medical University of Vienna, using the QuantSeq FWD protocol (Lexogen). 15 PCR cycles were used for library prep, as determined by qPCR. Libraries were QC-checked on a Bioanalyzer 2100 (Agilent) and quantified using Qubit dsDNA HS Assay (Invitrogen).