GSM8182720: Amygdala, sgRosa_2, 7D Stress and Fear Conditioning (RNA-seq); Mus musculus; RNA-Seq
RNA from neurons isolated with the Miltenyi MACS Adult Neuron Isolation Kit was isolated using the Picopure RNA Isolation Kit (Life Technologies) and quantified by the Qubit RNA HS Assay Kit (Thermo Fisher Scientific). 5 ng of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using the NEBNext Library Prep Kit according to the manufacturer's protocol.