Illumina HiSeq 3000 paired end sequencing; Human lymphedema tissues exhibit an inflammatory gene expression profile
Human lymphedema tissues exhibit an inflammatory gene expression profile
In total, 8 lipodermectomy specimens were collected. Samples were obtained from archival paraffin blocks of LD from patients treated at the Rangueil hospital, Toulouse, France between 2015 and 2016. Samples were selected as coded specimens under a protocol approved by the INSERM Institutional Review Board (DC-2008-452) and Research State Department (MinistÃ¨re de la recherche, ARS, CPP2, authorization AC-2008-452). When it was possible, some control arm tissue samples were collected for esthetical purpose. Total RNA from dermolipectomies were harvested and isolated by the phenol/chloroform extraction method using after tissue homogenization with a Precellys (Bertin technologies, France). Residual genomic DNA digestion was performed using the RNase free DNase I (ThermoFisher scientific, USA). RNA quality (RQN>8) was assessed with a 5300 Fragment Analyzer system (Agilent, USA). Total RNA was then depleted of ribosomal-RNA according to Eurofins genomics pipeline. The trimmed reads were then mapped to the Homo sapiens GRCh38.91 reference genome available on ENSEMBL using the STAR aligner v.2.5.2b