16S rRNA gene amplicon sequencing of lake shore water: Perturbation: Dilbit; Shore cleaner (SC) T=-3; Replicate 3
Water samples (1 L) were filtered using 0.22 m polyethersulfone membranes. DNA was extracted from filters using the Qiagen PowerWater kit following the manufacturer's instructions. Approximately 5ng of DNA were used as template to amplify the V4-V5 region of bacterial 16S rRNA genes with the primers 515F-Y and 926R.