GSM7899832: gun1-102-M-1; Arabidopsis thaliana; ncRNA-Seq
Total RNA from plants was isolated using Trizol (Invitrogen, Carlsbad, USA) and purified using Direct-zol™ RNA MiniPrep Plus columns (Zymo Research, Irvine, USA) according to the manufacturer's instructions. RNA integrity and quality was assessed by an Agilent 2100 Bioanalyzer (Agilent, Santa Clara, USA). RNA was depleted of ribosomal RNA with the RiboMinus Plant Kit for RNA-seq (Thermo Fisher Scientific), and rRNA-free RNA was cleaned by ethanol precipitation. The directional library was prepared at Novogene Biotech (Beijing, China) with the help of the NGS Stranded RNA Library Prep Set (Novogene Biotech, PT044).