GSM6630282: DEXA_6; Mus musculus; RNA-Seq
Mouse brain tumor tissues was sheared and homonized by RLT buffer from the RNA extraction kit (Cat.#74134, Qiagen). RNA samples were pretreated with DNase and 1 ug purified RNA was subjected to cDNA synthesis. Library preparation following the standard protocol from TruSeq® RNA LT Sample Prep Kit v2 (Illumina, US). Sequencing was carried out on the HiSeq 2500 platform (Illumina) with output 8-10 giga bit sequencing data per sample.