RNA-Seq of the HEK-293 cell clone 1 (replicate 2)
We stably overexpressed 3tRFCysGCA in HEK293 cells, by integrating one of the tRNACysGCA genes in the HEK293 genome. In this way, three HEK-293 clones were created. Total RNA was extracted from 2 replicates of each clone and 3 of the parental HEK293 cell line; poly(A) RNA was positively selected from each total RNA extract. Next, RNA-seq was performed in the DNBSEQ G50 platform.