GSM7401225: dHeps isolated from CCl4-injured mice, DMSO, Day16 [D1]; Mus musculus; ATAC-seq
5× 10^4 cells were used for every single reaction. Cells were washed in cold PBS and resuspended in 50 μl lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.5% NP-40) for 10 min, and nuclei were spun at 500 g for 10 min using a refrigerated centrifuge ATAC-seq libraries were prepared using Trueprep DNA library Prep Kit V2 for Illumina (vazyme).