GSM7765982: porcine blastoid 2, scRNAseq; Sus scrofa; RNA-Seq
Porcine blastoids with ICM-like and TE-like compartments and cavities were picked up manually by mouth pipette and dissociated into single cells by 0.25% trypsin-EDTA at 37°C for 10-20 min. The dissociation was terminated by 15% FBS, and samples were centrifuged at 500 g for 5 min. Cell pellets were resuspended in DMEM/F12 and filtered through a 40-μm cell strainer. Single-cell suspensions were carried out with 10x Genomics Chromium Single Cell Kit for Single-cell RNA-seq. RNA from the barcoded cells was subsequently reverse-transcribed and sequencing libraries constructed with reagents from a Chromium Single Cell 30 v2(v2 or v3, depends on project) reagent kit (10X Genomics) according to the manufacturer's instructions.