GSM8408982: Optic cup, AAV8-miR-429, rep1; Mus musculus; RNA-Seq
Eye samples were processed in QIAzol Lysis Reagent (QIAGEN). RNA was extracted using the miRNeasy extraction kit (QIAGEN), according to the manufacturer's instructions. RNAseq libraries were prepared according to manufacturer's instructions (TruSeq RNA Sample Preparation kit, Illumina, San Diego, CA) with an initial amount of 150 ng of total RNA