GSM7573930: BU3.10 erythroid; Homo sapiens; RNA-Seq
Freshly collected cells were passage through columns for cell removal to achieve a viability of >90%. Cells were resuspended in PBS/0/04% BSA (PBS without EDTA and Mg). 100 µl of single cell suspension at concentration of 700-1200 cells/µl was submitted to the sequencing service. Library was performed according to the manufacturers instruction (3' scRNAseq v3.1 10x Chromium)