stool samples from infants
Fecal samples were collected from diapers and placed in a sterile plastic container. The QIAamp  owerFecal Pro DNA kit (QIAGEN) was used to isolate bacterial DNA. The 16s rRNA gene was amplified using primers 338F and 806R, (V3-V4 hypervariable regions). The libraries were sequenced using the Illumina Miseq platform (Illumina, San Diego, CA).