GSM7574844: ISE6 cells, WT, 2; Ixodes scapularis; RNA-Seq
Total RNA was extracted using the All Prep DNA/RNA/PROTEIN Mini Kit (Qiagen, Hilden, Germany) following manufacturer's recommendations. The samples were subjected to polyA enrichment followed by fragmentation, first and second strand synthesis, adenylation of 3' ends, adapter ligation, DNA fragment enrichment, and real-time PCR quantification. TruSeq Stranded mRNA LT (Illumina) was used. Agilent 2100 Bioanalyzer (Agilent Technologies) assessed the size distribution of the amplified DNA.