snATAC-seq of adult human macular region of retina enriched for neurons
Samples included in this study were retinal tissues of donors from the Utah Lions Eye Bank collected within 6 hours post-mortem. All donors were screened for medical history, and only the ones with no of retinal diseases were used in this study. Post-mortem phenotyping with OCT were performed to confirm that there were no drusen, atrophy, or any other disease phenotypes on retina. With 4mm and 6mm disposable biopsy punches, macula and peripheral retina were collected and flash frozen in liquid nitrogen, and stored at -80C before nuclei isolation. Nuclei for snRNA-seq were isolated by fresh-made pre-chilled RNase-free lysis buffer. The frozen tissue was resuspended and triturated in lysis buffer and homogenized with a WheatonTM Dounce Tissue Grinder. To enrich the neuron nuclei, isolated macular retinal nuclei were stained with a mouse anti-NeuN monoclonal antibody. The stained nuclei were sorted with a BD Aria II flow sorter (70m nozzle).  Single cell ATAC Library was prepared according to Chromium Next GEM Single cell ATAC 511 Reagent kit v1.1 (10x Genomics).