GSM7979325: Tfap2a_CUT&RUN_24hpf_n1; Danio rerio; OTHER
Ten zebrafish embryos at 24hpf were dissociated in calcium-free wash buffer, washed three times with PBS, and filtered through a 40μm gauze. Approximately 250k cells were attached to CoA beads per sample, incubated with a 1:100 ratio of anti-Tfap2a or IgG antibody, and cleaved with pAG-MNase for 30 minutes. The cut fragments were purified, and library preparation was conducted using KAPA kits. Sequencing was performed with 150PE reads at 20 million reads per sample Library construction was performed using the KAPA Biosciences kit following the manufacturer's protocol.