Illumina PE Hi-C library of Zymo pooled gut
A proximity ligation (Hi-C) library was prepared from an aliquot of the Fecal Reference standard using the ProxiMeta Hi-C v4.0 Kit from Phase Genomics according to the manufacturer provided protocol (Lieberman-Aiden et al. 2009). Briefly, intact cells were crosslinked using a formaldehyde solution, simultaneously digested using the Sau3AI and MlucI restriction enzymes, and proximity ligated with biotinylated nucleotides to create chimeric molecules composed of fragments from different regions of microbial genomes that were physically proximal in vivo. Proximity ligated DNA molecules were pulled down with streptavidin beads and processed into an Illumina-compatible sequencing library. Sequencing was performed on an Illumina NovaSeq with PE150 read pairs, producing 181.7M reads in total.