GSM6632233: BZH1; Mus musculus; Bisulfite-Seq
Total DNA was extracted using QIAamp Fast DNA Tissue Kit (Qiagen, Dusseldorf, Germany) following the manufacturer's procedure. The quantity of DNA was measured by reading A260/280 ratios by spectrophotometer. When A260/280 ratios located range 1.8 to 2.0, DNA was available. Whole genome bisulfite sequencing libraries were constructed using the Acegen Bisulfite-Seq Library Prep Kit (Acegen, Cat. No. AG0311) according to the manufacturer's protocol. Briefly, 1 μg of genomic DNA spiked with 1 ng unmethylated Lambda DNA was fragmented by sonication to a mean size of approximately 200-500 bp, then end-repaired, 5'-phosphorylated, 3'-dA-tailed and ligated to 5-methylcytosine-modiﬁed adapters. After bisulﬁte treatment, the DNA was ampliﬁed with 10 cycles of PCR using Illumina 8-bp dual index primers.