GSM7696010: hiPSC Pro-TGG upstream edit BRF1 ChIP-Seq rep1; Homo sapiens; ChIP-Seq
Cells were fixed with 0.8% methanol-free formaldehyde in DMEM for 10 min, followed by quenching with 0.125 M glycine for 5 min. Cells were resuspended in Farnham buffer (5 mM PIPES, pH 8; 85 mM KCl; 0.5% IGEPAL-CA 630), followed by snap freezing in liquid nitrogen. All buffers were supplemented with cOmplete™ EDTA-free Protease Inhibitor Cocktail before use. Chromatin was isolated and sheared following the NEXSON protocol. Frozen cell pellets were thawed on ice and sonicated for 2 min in 1- 