GSM7091474: Fresh4 hours (10xx21YW_X_A04_S4); Mus musculus; RNA-Seq
RNA was extracted using the RNEasy Plus Micro Kit (Qiagen) according to the manufacturers recommendations. Smart-Seq2 cDNA synthesis was performed on isolated follicle RNA with Maxima H Minus reverse transcriptase (ThermoFisher). Whole transcriptome amplified (WTA) product was purified with AMPure XP beads (Beckman Coulter), quantified with Quanit-iT PicoGreen dsDNA Assay (ThermoFisher), normalized and used for preparing paired-end sequencing libraries with Nextera XT (Illumina, #FC-131) according to the manufacturer's instructions. Illumina NextSeq500/550