GSM8138733: Transitional_wildtype_ATAC_day30_biol rep1; Mus musculus; ATAC-seq
Single cell suspension was prepared from spleens of day 30 LCMV clone 13 infected mice or from uninfected mice for naive samples. GP33+ antigen specific CD8+ T cells were FACS sorted and the assay for transposase-accessible chromatin with high sequencing was performed on the cells according to the published protocol (Grandi, F.C., Modi, H., Kampman, L. et al. Chromatin accessibility profiling by ATAC-seq. Nat Protoc 17, 1518–1552 (2022). https://doi.org/10.1038/s41596-022-00692-9). Briefly, nuclei was isolated from FACS sorted antigen specific CD8+ T cell subsets and treated with Tn5 transposase for 30 mins at 37 degrees celsius. Transposed DNA was isolated, PCR amplified using barcoded primers. Eluted DNA was PCR amplified with barcoded primers. Amplified DNA was purified with AMPure XP magnetic beads.