GSM8229014: PC9 cells, normoxic device, 24 hr, rep2; Homo sapiens; RNA-Seq
RNA was extracted using the RNeasy Plus Micro Kit (Qiagen) following the manufacturer's instructions.  Quality of the RNA was assessed using Agilent 2100 Bioanalyzer (Agilent Technologies). Only the RNA samples that have passed the QC were used. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. Libraries were constructed with the NEBNExt Ultra II library kit (New England BioLabs Inc)