GSM6937729: S32; Mus musculus; OTHER
Spatial transcriptomics in murine placentae. At E18.5 placentas were dissected and preserved in RNA-later at -25C for 18 hours. Tissues were dehydrated by incubation in 30% sucrose at 4oC for 6 hours. At the Baylor College of Medicine Pathology and Histology Core placentas were fresh-frozen in optimal cutting temperature compound (OCT) with a liquid nitrogen and isopentane bath. Tissue blocks were cryosectioned at 10 microns for quality control H&E staining and 10x Genomics Visium Tissue Optimization. The Tissue Optimization slide revealed optimal permeabilization was observed after 12 minutes. Finally, the tissue blocks were cryosectioned and placed on Visium Gene Expression slides (v2) for H&E staining and Visium spatial transcriptomics library preparation following the manufacturer's instructions. Visium libraries were sequenced (Illumina, NovaSeq 6000) at the Baylor College of Medicine Genomic and RNA Profiling Core. Visium (v2)