GSM7091670: Late Recurrence, Patient 2, Post-Chemo; Homo sapiens; RNA-Seq
The Chemagic360 system (Perkin Elmer) was used to extract total nucleic acid from the tumor samples with TURBO Dnase (Invitrogen) treatment to obtain pure RNA RNA libraries for RNA-seq were prepared using KAPA mRNA HyperPrep Kit (KAPA Biosystem, Wilmington, MA) for Illumina following manufacturer's protocols.