GSM7664343: TSC1null_DMSO_rep3_Total; Homo sapiens; RNA-Seq
Fractions corresponding to mRNA associated with more than two ribosomes were pooled and the RNA extracted using TRIzol (Thermo Fisher, Waltham, MA) according to the manufacturer's protocol. Prior to loading samples on the sucrose gradient, RNA was extracted from 10% of the lysate using TRIzol, and the resulting RNA was denoted as total RNA. Illumina v2.5 Kit