GSM7567137: d6_1 CTRL; Homo sapiens; RNA-Seq
Cells were disrupted directly on culture wells with QlAzol Lysis Reagent (Qiagen, MD, USA). Total RNA was extracted and purified with miRNeasy Mini Kit (Qiagen, Hilden, Germany) including an additional On-Column DNase Digestion with the RNase-Free DNase Set. Library preparation from 800 ng of total RNA was performed according to Illumina methodology (stranded total RNA prep with Ribo-Zero Plus Reference Guide, Illumina, San Diego, CA, USA).