GSM8229441: RNA-seq, SNU1079 in vitro, sgCtrl, AG120, Replicate 2; Homo sapiens; RNA-Seq
Single-cell suspensions from cells cultured in a monolayer were homogenized in 300 to 600 μL of TRIzol. RNA was extracted from homogenized samples using the Direct-zol RNA Miniprep Plus Kit in accordance with the manufacturer's suggested protocol. The library was constructed by NEB RNA Ultra™ II Directional kit