GSM6631130: hPGC_F_UNC1999_BRC2197; Homo sapiens; RNA-Seq
RNA was extracted using the PicoPure RNA Isolation Kit including an on-column DNase 1 digest (Qiagen 79254) following the manufacturer's instruction. RNA-seq libraries were generated from 2 to 5 ng of total RNA using NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB, E6420) and NEBNext®Multiplex Oligos for Illumina (NEB, E7335S). Library quality was accessed by TapeStation 2200 (Agilent) with High Sensitivity D1000 ScreenTape (Agilent), quantified and multiplexed.