HM-Treatment-4d
The total RNA of 30 DX and HM leave samples (3 replicates  10) was extracted using TRIzol Reagent (Invitrogen, USA) as mentioned in protocol, RNA quality and quantity was then determined using 5300 Bioanalyzer (Aligent) and ND-2000 (NanoDrop Technologies), respectively. A total of 30 RNA-sequencing libraries were generated using 1 L of total RNA following Illumina Stranded mRNA Prep, Ligation form Illumina (San Diego ,CA) by Majorbio Biological Technology (Shanghai, China).