Metabarcoding of nematodes of Store Mosse
Index PCR was performed where unique indices were attached to amplicons of each sample. Library size distribution was checked by running on Agilent Technologies 2100 Bioanalyzer using a DNA 1000 chip. Quantification of the library was performed using qPCR according to the Illumina qPCR quantification protocol guide. The samples were then multiplexed, before Illumina MiSeq 2x300 bp sequencing.