GSM7405335: TK1_10µM Karanjin treated; Homo sapiens; RNA-Seq
Total RNA was isolated using RNA extraction reagent prepared in-house as per Chomczynski and Sacchi, with modifications mRNA enrichment was performed using KAPA mRNA capture kit. Libraries were prepared according to instructions accompanying the KAPA RNA Hyper prep kit (Roche, Switzerland). mRNA was fragmented and reverse transcribed using oligo-dT primers to synthesize cDNA. The double-stranded cDNAs were end-repaired, polyadenylated, and ligated with adapter sequences. Sequencing was performed using Nextseq2K Platform in 150 paired-end reads length format.