GSM6373867: Timeseries_ATACseq_OCT4-FKBP_2H_Rep1; Mus musculus; ATAC-seq
Cells were harvested and washed 1X in cold PBS. 50,000 cells were counted and nuclei were lysed using 2x lysis buffer (1M Tris-HCl pH 7.5, 5M NaCl, 1M MgCl2, 10% IGEPAL). Cells were spun down incubated for 1h at 37°C in tagmentation buffer (20 mM   Tris(hydroxymethyl)aminomethane; 10 mM MgCl2, 20% dimethylformamide, brought at pH 7.6 with acetic acid) containing custom Tn5. For amplification of the tagmented DNA, two rounds of PCR were used using KAPA kit amplification protocol and sequencing adapters were included during the PCRs for library preparation. Fragments smaller than 700 bp were purified using SPRI beads selection. Quality of the libraries was checked on a Bioanalyzer before sequencing.