GSM7051079: shRad21_2_2 [R3_2]; Mus musculus; RNA-Seq
Total RNA from cells was extracted using the RNeasy® Mini or Micro Kit (Qiagen). The mRNA is fragmented randomly by adding fragmentation buffer, then the cDNA is synthesized by using mRNA template and random hexamers primer, after which a custom second-strand synthesis buffer (Illumina) , dNTPs, RNase H and DNA polymerase I are added to initiate the second-strand synthesis. Second, after a series of terminal repair, a ligation and sequencing adaptor ligation, the double-stranded cDNA library is completed through size selection and PCR enrichment.