GSM8278955: muscle, LPI, 2 days, rep 3; Rattus; RNA-Seq
Rats were deeply anesthetized with isoflurane, transcardially perfused with nuclease-free saline (pH 7.4). The incised skin and muscle tissue were rapidly dissected, the C3-C6 DRGs of LPI and C2-C5 DRGs of SMIR were then extracted. All samples were immediately transferred to ice-cold RNAlater solution and refrigerated at 4°C for 48 hours. Then samples were removed from RNAlater solution, placed into fresh 1.5 mL conical tubes and stored at -80℃. Total RNA was isolated, then Gel electrophoresis and Qubit were used to exam the RNA quality and RNA integrity number (RIN). For RNA sequencing, RNA samples from seven to nine biological replicates at each time point were separated into three independent pools. Strand-specific libraries were constructed using the TruSeq RNA sample preparation kit, and sequencing was carried out using the Illumina Novaseq 6000 instrument. Strand-specific libraries were constructed using the TruSeq RNA sample preparation kit (RS-122-2001, Illumina, San Diego, CA, USA)