GSM7091601: IL15 CBNK GBM Day 9  coculture 124610 RNA; Homo sapiens; RNA-Seq
For sc-RNA seq, single cell capture, barcoding and library preparation were performed following the 10X Genomics Single Cell Chromium 3' protocols (CG000183, V3.1). Ten libraries were pooled to give a final concentration of 10 nM. Pooled samples underwent further qPCR to determine the final concentration before submission for sequencing with the NovaSeq6000 sequencer using the S2 100 cycles flow cell and sequenced for 28 cycles for read1, 8 cycles for i7 index, and 91 cycles for read 2 through the ATGC (Advanced Technology Genomics Core) core at MD Anderson. By applying RNA  and ATAC sequencing, we want to investigate the epigenetic factors that contributing for IL-21-induced memory formation in NK cells