GSM7663761: lung, ILC2s, scRNA-seq; Homo sapiens; RNA-Seq
Freshly collected human lung tissues were cut into small pieces. The tissues were digested at 37°C for 30 min in RPMI 1640 medium containing Liberase (0.1 mg/ml)/deoxyribonuclease I (20 μg/ml)/10 mM Hepes/3% heat-inactivated fetal bovine serum and then mashed through 70-μm cell strainers. The cells were stained with intended FACS antibodies and processed on the BD FACSAria Fusion single cell lysis to release RNA, reverse transcription, amplification, pooling and purification, quality control, DNA fragmentation, construction of sequencing library