GSM7091821: Acanthaomeba castellanii, E3; Acanthamoeba castellanii; RNA-Seq
The total RNA of A. castellanii was extracted with Direct-zolTM RNA MiniPrep (AYMO RESEAECH) according to the manufacturer's protocol. The samples were treated with DNase I at 37°C for 30 minutes. DNase I was removed by phenol-chloroform extraction and total RNA was reprecipitated using sodium acetate and ethanol. The total RNA was eluted in nucleic acid-free water. The protocol for strand-specific sample preparation was ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicentre, USA).