GSM8138628: Knockout_rep1; Pseudomonas syringae pv. syringae; RNA-Seq
Tubes of 5 mL cultures of Pss UMAF0158 wildtype and Δmgo1 were inoculated with 10 μL of overnight culture and grown for 24 hr. Three biological replicates each of wildtype and Δmgo1 were grown. After 24 hr, 1 mL of each culture was removed for RNA extraction. For RNA extraction, cells were harvested by centrifugation for 30 min at 4˚C, 3500 x g. RNA was extracted with TRIzol (ThermoFisher Scientific) and purified using Direct-zol™ RNA Miniprep Plus kit (Zymo Research), as per the manufacturer's protocol. Sequencing libraries were prepared using the Novogene Prokaryotic RNA Library kit according to the manufacturer's instructions.