GSM7575381: MV411_InVitro_minusDox_Rep1; Homo sapiens; OTHER
PCR of genomic DNA was performed using ExTaq (Takara) using custom designed primers with Nextera i5 and i7 index sequences (supplementary table X) to amplify the mir30 insert containing the shRNA. Amplicons were electrophoresed on an agarose gel and DNA was purified using the QIAquick gel extraction kit (QIAGEN) according to manufacturers instructions and further purified by ampure (Beckman Coulter). Samples were pooled and analysed on a Next Seq 2000 75 using a NextSeq 500/550 High output kit