DDP4_S4_H2VC3_L002
seeds were colected and soaked in water at room temperaturefor four days, then haploid megagametophytes were dissected from each seed. DNA wasextracted with a Qiagen DNeasy mini-prep Plant kit, and DNA quality and concentrationwere evaluated using nanodrop and picogreen on a Qubit 2.0 Fluorometer, respectively. Se-quencing libraries were constructed using Illuminas TruSeq Nano DNA Library Prep Kit .Prior to amplification, DNA was fragmented (200 ng starting material and 550 bp target insertsize), followed by end repair and size selection of fragments, adenylation of 3ends, andadapter ligation. PCR enrichment was performed in eight cycles. Barcoded libraries werecombined into normalized pools and sequenced to >10 X coverage on an Illumina HiSeq 3000using 150 bp paired-end reads at the University of California-Davis Genome Center