GSM7567163: fibroblast, unstimulated, rep2; Homo sapiens; RNA-Seq
total RNA was extracted using TRIzol reagent (Thermofisher) according to manufacturer's instructions The quality of libraries was assessed by checking the distribution of the fragment size using the Agilent 2100 bioanalyzer. The double stranded PCR products were then heat-denatured and circularized by the splint oligo sequence. The single strand circle DNA (ssCir DNA) was formatted as the final library.