GSM7401088: I_24h_2_M; Mus musculus; RNA-Seq
RNA from frozen injection sites (muscles) were isolated by homogenization in TRI-reagent (Sigma, T9424) using Omni Tissue Homogenizer (TH) equipped with 7 x 15 mm Saw Tooth (Fine) Generator Probe, homogenous mixtures were heated to 60 °C and further processed according to the manufacturer's instructions. Libraries were prepared using a KAPA RNA HyperPrep Kit with RiboErase (KAPA Biosystems, #08098140702), according to the manufacturer. KAPA UDI Indexes (KAPA Biosystems, #08098140702) were used. Library quality was assessed using chip electrophoresis performed on an Agilent 2100 Bioanalyzer (Agilent Technologies, Inc.).