GSM6820627: regeneration, 0hpa; Schmidtea mediterranea; RNA-Seq
For regenerated fragments, planarians were chopped and dissociated in CMFB (CMF+1% FBS) on a cold plate (4 °C). After dissociation, cells were pelleted by centrifugation (290g for 5 min at 4 °C). Before loading onto the flow cytometry sorter, cells were strained through a 40um strainer and stained with DAPI (1:1000; Beyotime) and DRAQ5 (1:1000; BioStatus). Afterward, the collected live cells were washed and suspended with CMFB buffer. Around 20,000 cells were loaded into 10x Genomics' Chromium platform for making single-cell libraries. The deep sequencing of samples (2-4 million reads per cell) was performed using the Novaseq-150PE sequencer.