GSM8182170: 19739X3 MDA-MB-468 cells 3D Multiplex; Homo sapiens; RNA-Seq
Single-cell suspension of MDA-MB-468 organoids were generated by sequetional dissociation with dispase and trypsin and resuspended in phosphate buffer saline + 0.04% BSA. Cells were filtered through a 40micron filter and counted on a Countess 2 (Invitrogen, Carlsbad, CA) Chromium Next GEM Single Cell 3' Kit v3.1 (PN-1000268) was used to barcode individual cells with 16nt 10x Barcode, to tag cell specific transcript molecules with 12 nt Unique Molecular Identifier (UMI) and to capture poly (A) mRNA with 30 nt ploy(dT) sequence according to the manufacturer's instructions. 15k cells were targeted for sequencing.