GSM8086642: Liver, wild, M37976, RNA-Seq; Mus musculus; RNA-Seq
We extracted RNA from fresh mouse using an AllPrep DNA/RNA kit (Qiagen). We placed 5-10 mg tissue into 350 μL RLT Plus Buffer in a 2 mL tube with a 5 mm stainless steel bead and homogenised with a TissueLyzer (two 2 min rounds, 30 Hz). We then followed manufacturer's instructions to isolate RNA, eluting in 30 μL RNase-free water.  Illumina stranded mRNA library prep