GSM7663598: HT,input; Mus musculus; ChIP-Seq
6x10^6 cells were cross-linked with 1% formaldehyde for 15 mins and quenched with glycine. Genomic DNA was sheared by sonication with Qsonica Q800R2 sonicator for 15mins (15s on: 45s off). Input DNA were taken and stored at -80 ℃. Antibodies were first incubated with Protein A beads and Protein G beads, and the beads-antibody complex were used for immunoprecipitation. After reverse cross-linking, DNA was purified by a HiPure Gel DNA Mini Kit. The sequencing library was prepared with VAHTS Universal DNA Library Prep Kit for Illumina V3 following the manufacturer's manual.