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Total RNA of each sample was extracted from the honeybee larvae according to the instruction manual of the TRlzol Reagent (Life technologies, California, USA). The mRNA was isolated by NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490). The cDNA library was constructed following the manufacturers instructions of NEBNext Ultra RNA Library Prep Kit for Illumina NEB, E7530 and NEBNext Multiplex Oligos for Illumina.