DNA-seq of Juglans regia
The Plant Genomic DNA Kits (Tiangen, Beijing, China) were used to extract genomic DNA from walnut young leaves. The sample testing, library construction, and sequencing were performed by Novogene Bioinformatics Technology Co. Ltd., Beijing. The library preparation of DNA fragments was completed through terminal repair, addition of ployA tail, addition of sequencing adapter, purification, and PCR amplification using TruSeq Library Construction Kit according to the manufacturer's instructions. After accurately quantifying the effective concentration of the library using Q-PCR method (library effective concentration > 2nM). All sequencing was conducted on the Illumina PE150 platform