GSM7899144: PBMC_bulkRNA-seq Patient_2; Homo sapiens; RNA-Seq
For snap frozen tissue samples, samples were shredded in RNeasy lysis buffer in a Mixer Mill prior RNA Isolation. For PBMC samples were lysed with RNeasy lysis buffer. Subsequently, RNA was extracted for both tissue and PBMC samples using the RNeasy Mini Kit (74106, Qiagen) according to the manufacturer's instructions. RNA libraries were prepped with the NEBNext Ultra II Directional RNA Library Prep Kit (NEB, E7760L) according to the manufacturer's instructions.