GSM7663859: MSC_2942KO; Mus musculus; RNA-Seq
MSCs and OBs were sorted from wild-type and miR-9-1-deficient mice, lysed in TRIzol (LifeTechnologies), and mRNAs were then isolated using NEBNext poly(A) mRNA Magnetic Isolation Module (New England Biolabs). 0.5 ug of total RNA was used for the construction of sequencing libraries. The libraries were constructed using NEBNext Ultra RNA library Prep kit for Illumina (New England Biolabs) and quantified using Kapa Library Quantification Kit (KapaBiosystems) and QubitFluorometer (ThermoFisher).