GSM7567455: 324_CTRL+CP+MET; Homo sapiens; RNA-Seq
RNA was extracted from SKNBE(2) xenograft tissue using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen) according to the manufacturer´s instruction Sequencing libraries were be prepared at the Core Facility Genomics, Medical University of Vienna using the NEBNext Poly(A) mRNA Magnetic Isolation Module and the NEBNext UltraTM II Directional RNA Library Prep Kit for Illumina according to manufacturer's protocols (New England Biolabs). Libraries were QC-checked on a Bioanalyzer 2100 (Agilent) using a High Sensitivity DNA Kit for correct insert size and quantified using Qubit dsDNA HS Assay (Invitrogen). Pooled libraries were sequenced on 2 flowcells of a NextSeq500 instrument (Illumina) in 1x75bp single-end sequencing mode.