GSM7294725: Del-c11.8-Rad21-ChIP; Mus musculus; Mus spretus; ChIP-Seq
ChIP was performed using fixed chromatin from Patski cells. DNA from ChIP pull-down chromatin fractions were de-crosslinked and purified using QIAquick PCR purification kit. Sequencing libraries were prepared each from three or  four pooled independent ChIP samples from Patski cells using the Illumina Truseq ChIP sample prep kit. Size selection of the library was done by two-step Ampure bead purification and purification of DNA in the 300-600bp range.