GSM7092520: biol rep 3, sample 2, hiFT cells, sorted for fast cycling; Homo sapiens; RNA-Seq
RNA was extracted as per the manufacter's protocol using a 10x Genomics v3 kit. We generated gel beads-in-emulsion (GEMs) using the 10X Chromium system and a Chromium Next GEM Single Cell 3' Reagent Kit v3.1 as per the manufecturer's protocol, aiming for 10,000 cells for recovery. Single cellRNA-sequencing libraries were constructed as per the manufacter's protcol using a 10x Genomics single-cell RNA-sequencing kit v3. We extracted cDNA according to the post-GEM RT-cleanup instructions and used 10uL of amplified cDNA for fragmentation, end repair, poly A-tailing, adapter ligation, and 10x sample indexing.