GSM7663136: PR+/+ (heterozygous non-inducible), mouse 2 [IAO265]; Mus musculus; RNA-Seq
Total RNA was extracted from liver tissue using TRIzol reagent (Invitrogen), according to the manufacturer's protocols. A portion of the RNA was purified a second time with the RNA Clean & Concentrator-5 kit (Zymo Research), according to manufacturer's instructions, and submitted to standard quality controls. 3'-end RNA-seq; RNA Quality scores were 7.2 on average (range 7.0-7.4). Sequencing libraries were prepared from 300ng total RNA samples with the "QuantSeq 3' mRNA-Seq Library Prep Kit (FWD) for Illumina" (Lexogen, Cat.No. 015) by following manufacturer instructions. {This kit generates directional libraries stranded in the sense orientation (the read1 (the only read in single read format) has the sense orientation (--library-type fr-secondstrand in TopHat, --stranded=yes in HTSeq).}