16S rRNA-Seq of environmental metagenome: seawater sample after 48 hour mesocosm experiment
Seawater sample after 48h duration of mesocsom experiment (500 ml) filtrated onto 0.2 m pore-sized polycarbonate filters. For experimental details see corresponding publication. Extraction with Qiagen PowerSoil kit. Triple PCR amplification of the V3-V4 region of the 16S rRNA gene using the Bac341F primer (50-CCT ACG GGN GGC WGC AG-30) and the Bac805R primer (50-GAC TAC HVG GGT ATC TAA TCC-30) with overhang adapters. The PCR product was cleaned AMPure XP beads and normalized to 4 ng/l.