GSM6692836: shControl_1; Homo sapiens; RNA-Seq
Total RNA was extracted using Trizol Reagent (Invitrogen). After the recovery of the aqueous phase using chlorophorm, a DNAse treatment was performed using the Mini RNeasy kit (Qiagen) following manufacturer instructions.  cDNA libraries were generated using the Illumina TruSeq RNA Sample Preparation Kit, and sequenced on a Illumina HiSeq2500 sequencer. Total RNA from tissues or cells was extracted using the TRIZOL Reagent (Invitrogen). After recovery of the aqueous phase using chloroform, DNase treatment and further purification were performed using the RNeasy Mini Kit (Qiagen), following the manufacturer's instructions.  RNA sequencing (RNA-seq) was conducted by the CNIO Genomics Unit. Total RNA (1 µg) from samples was used. The average sample RNA Integrity Number was 9.65 (Agilent 2100 Bioanalyzer). cDNA libraries were generated using the Illumina TruSeq RNA Sample Preparation kit and sequenced on an Illumina HiSeq 2500 sequencer.