Illumina NovaSeq 6000 paired end sequencing; Inflammatory signals from fatty bone marrow support DNMT3a-driven clonal hematopoiesis (NOD/SCID/IL-2Rgc-null subset)
Inflammatory signals from fatty bone marrow support DNMT3a-driven clonal hematopoiesis (NOD/SCID/IL-2Rgc-null subset)
Femora and tibiae, irradiated and non irradiated were isolated from two months DNMT3AMut, bone marrow was flushed in to 2 tubes. We have adopted a protocol developed by Rosen et.al. The protocol combines a density gradient centrifugation method optimized for adipose tissue followed by nuclei “cleanup” step by sorting. Nuclei were sorted to low binding Eppendorf tubes and library was prepared by the 10X genomics V3 3' kit. Libraries were prepared by the 10X genomics V3 3' kit.