MSfatW
After the total RNA sample passes the quality control test, use a reagent kit to remove rRNA, add Fragmentation Buffer to the resulting mRNA to fragment it into short fragments, then use the fragmented RNA as a template to synthesize the first strand of cDNA with a six-base random primer. Replace dTTP with dUTP, then add adapter sequences on both ends, along with buffer solution, dNTPs, RNaseH, and DNA Polymerase I to synthesize the second strand of cDNA.