GSM7717120: whole body larval fathead minnow, 6PPD, biol rep2 [6PPD_C_A04]; Pimephales promelas; RNA-Seq
Exposure media was removed from each well, 100 ul of Omega Biotek MagBind Total RNA homogenization buffer was added and individual fish were homogenized using a bead mill at 30 Hz for 2 min. Whole-body homogenates were extracted using MagMAX-96 Total RNA Isolation kits (ThermoFischer Scientific AM1830) and RNA quality verified using an Agilent Tapestation (Agilent, Santa Clara, CA). Complementary DNA libraries were prepared from 250 ng total RNA using SENSE mRNA-Seq Library Prep kits (Lexogen).