GSM7900618: mCCD control 2 – rep 3; Mus musculus; RNA-Seq
RNA was extracted using RNeasy Mini Kit (Qiagen). RNA quality was determined by fragment analyzer and all samples were considered good quality with an RNA quality number >8.3. RNA quality was assessed on a Fragment Analyzer (Agilent Technologies). The RNAs had RQNs between 7.8 and 10. RNA-seq libraries were prepared from 108 ng of total RNA with the Illumina Stranded mRNA Prep reagents (Illumina). Libraries were quantified by a fluorometric method (QubIT, Life Technologies) and their quality assessed on a Fragment Analyzer (Agilent Technologies). Sequencing was performed on an Illumina NovaSeq 6000 for 100 cycles single read. Sequencing data were demultiplexed using the bcl2fastq2 Conversion Software (version 2.20, Illumina