TCR-seq for human T cells: Sample HIV13
TCR-seq for human T cells; PMCID: PMC6878759; FACS-sorted cells were lysed, and total RNA was purified using AllPrep DNA/RNA Micro Kit (Qiagen). 30% of the purified RNA was used for library generation and sequencing. Consensus sequences were constructed within each molecular identifier (MID) group. Consensus TCR sequences were subjected to the CDR3 blast module of MIGEC  to assign V and J alleles and parse out the CDR3 sequence.