GSM6696243: C57BL6/J T cells, GW CRISPR library, replicate B, GFP bin 1; Mus musculus; OTHER
Genomic DNA was extracted by phenol:chloroform:isoamyl alcohol DNA isolation. Libraries were PCR amplified around the gRNA sequence from genomic DNA. A total of 5 ug of gDNA was used a template for each PCR reaction, six PCR reactios were run for each sample.