GSM7666033: SWH1_mmlib_30min_2rep; synthetic construct; OTHER
phenol chloroform extraction of DNA single-stranded 3' end DNA fragments were converted into double-stranded DNA by Bst polymerase (NEB Bst 2.0 WarmStart). And NGS library was prepared with NEBUltraII kit (NEBNext Ultra II DNA Library Prep Kit) for Illumina sequecing.