GSM7719642: iPSC-EC Static_01; Homo sapiens; RNA-Seq
An Rneasy Mini Kit (Qiagen) was used with on-column DNA digestion to extract the RNA samples from the cells on chips. 1.2 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using a SMART-Seq v4 Ultra Low Input RNA Kit (Takara) following the manufacturers' protocol.