16S Metagenomic-perlite-rich soil-non-grazed area 3
DNA extracted from perlite-rich soil using ZymoBIOMICS DNA Miniprep Kit. NGS Library Prep Kit with Quick-16S Primer Set V3-V4 (forward primer: 5-CCTACGGGDGGCWGCAG-3, 5-CCTAYGGGGYGCWGCAG-3 and reverse primers 5-GACTACNVGGGTMTCTAATCC-3. The forward primer is a mixture of the two sequences