Amplicon sequencing of Raji-J3 cells: 6 months, Experiment #2
Genomic DNA was isolated from sorted Raji cells 6 months after editing with sg05-J3 AAV6 homology donor vectors, amplified by PCR to incorporate sample barcodes and UMI tags in a single reaction (based on methods of MAUI-seq), and amplicons were column purified and sequenced.