GSM6939600: LIVER_3218_MOET; Bos taurus; Bisulfite-Seq
Tissues samples were shipped in dry ice to the BGI TECH SOLUTIONS Company (Hong Kong), which performed the gDNA extraction, quality control, library preparation, and sequencing. All samples met the requirements for library preparation (GC content between 35%-65% for extracted gDNA). The gDNA samples were subjected to Whole Genome Bisulfite Sequencing (WGBS), through bisulfite library preparation and PE100 sequencing with 45Gb clean data per sample on DNBSEQ. After library preparation and sequencing, raw data with adapter sequences or low-quality sequences were filtered to remove contamination and obtain valid data. This step was completed by the SOAPnuke software developed by the BGI Company.