Hi-C Illumina HiSeq X Ten sequencing of Coryphaenoides rudis
Total RNA was extracted from muscle tissue samples using the Qiagen RNeasy Mini Kit according to the manufacturers protocol. RNA quality and quantity were assessed using an Agilent 2100 Bioanalyzer and Qubit fluorometer, respectively. rRNA was removed using the Ribo-Zero rRNA Removal Kit to enrich for mRNA. The remaining RNA was fragmented and reverse-transcribed into cDNA using random hexamer primers. cDNA libraries were constructed using the Illumina TruSeq RNA Library Prep Kit according to the manufacturers instructions. Libraries were indexed, pooled, and sequenced on an Illumina HiSeq X Ten platform to generate 2 x 150 bp paired-end reads.