Nanopore long-read metabarcoding of a bacterial mock community with the 16S-27F/23S-2241R primer set, using the current V14 sequencing chemistry (R10.4.1 flowcells).
A bacterial mock community was amplified with the 16S-27F/23S-2241R primer set. Library prep was then performed using the SQK-LSK114 ligation kit and the sequencing was carried out using the current V14 sequencing chemistry (R10.4.1 Flongle flowcells). Raw pod5 files were basecalled using Dorado.