GSM7873783: mESC_GLORI_rep2; Mus musculus; RNA-Seq
After extracting total RNA from the samples using TRIzol (Thermo Fisher Scientifific, 15596026), mRNA was isolated via a Dynabeads mRNA Purifification Kit (Thermo Fisher Scientifific, 61006) with genomic DNA removal using TuRBO DNase kit (Thermo Fisher, AM2238). GLORI experiments were conducted following instructions provided in Cong Liu et al., 202214, with an adjusted incubation temperature in deamination step of 25 ℃.