Mastitis Buffalo
PCR amplification of 16S rRNA genes was conducted using the 2X EmeraldAmp MAX PCR Master Mix (code no. RR320A, Takara) in a total volume of 25l containing inner primer pairs (50nM each) and the barcoded outer primer mixture (3%) from the PCR Barcoding Kit (SQK-PBK004; Oxford Nanopore Technologies, Oxford, UK).