GSM6384037: BOV13; Bos taurus; RNA-Seq
Total RNA was isolated from frozen tissues (60-100 mg) using TRIzol reagent (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions. The total RNA concentration was determined using NanoDrop One (Thermo Fisher Scientific, Madison, WI). RNA quality was determined with the Agilent 2100 Bioanalyzer (Agilent Technologies, Massy, France). RNA libraries were prepared for sequencing using standard lllumina protocols