GSM8409010: Proliferating IMR90 shATG7 dox+ rep2; Homo sapiens; RNA-Seq
Total RNAs were isolated using the acid phenol extraction method. One microgram of high-quality RNA samples (RIN > 7.0) was used to construct RNA-seq libraries using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina). Total RNA integrity was checked using an Agilent 2200 TapeStation. RNA-seq was performed on an Illumina NovaSeq 6000 sequencer at Macrogen.