GSM8409086: Patient AML018, blood, time point 1, Batch 1; Homo sapiens; RNA-Seq
Cell pellets were lysed in TriZol and RNA was extracted Quick-RNA MiniPrep Kit by Zymo according to the manufacturers instructions. CDNA libraries were obtained according to Picelli, S. et al. Genome Res. 24, 2033–2040 (2014). Illumina library was obtained via tagmentation using Illumina Nextera Kit. All samples were sequenced in a single run on a NovaSeq6000 (single read, 100bp, depth 20 Mio reads per sample)