GSM7845352: DT; Mus musculus; RNA-Seq
A total amount of 1-3μg RNA per sample was used as input material for the RNA  sample preparations. Sequencing libraries were generated using VAHTS Universal  V6 RNA-seq Library Prep Kit for Illumina ® （NR604-01/02）following the  manufacturer's recommendations and index codes were added to attribute sequences to  each sample. Briefly, mRNA was purified from total RNA using poly-T oligo-attached  magnetic beads. Then we added fragmentation buffer to break the mRNA into short  fragments. First strand cDNA was synthesized using random hexamer primer and  RNase H. Second strand cDNA synthesis was subsequently performed using buffer,  dNTPs, DNA polymerase I and RNase H. And then, the double stranded cDNA was  purified by AMPure P beads or QiaQuick PCR kit. The purified double stranded cDNA  was repaired at the end, added a tail and connected to the sequencing connector, then  the fragment size was selected, and finally the final cDNA library was obtained by PCR  enrichment. RAN library were prepared for sequencing using standard Illumina protocols