Direct RNA-Seq of mouse embryonic stem cells derived motor neurones - WT
Total RNA was enriched in mRNA by poly-A selection and 500ng of poly-A+ RNA was used to prepare the sequencing library according to ONT protocol for direct RNA-seq SQK-RNA002. The library was sequenced on a R9.4.1 flowcell (FLO-MIN106) on a GridION machine and basecalled with Guppy 3.2.10.