RNA-Seq aorta WT control mus musculus
Total RNA prepared from aortas taken from Brn-3b KO male mutants and age-matched wild-type controls (3/set) were analysed for quality controls (QC) using NanoDrop and Agilent Bioanalyser 2100. Purified RNA was used for cDNA synthesis and library preparation with KAPA mRNA HyperPrep Kit (p/n KK8580). Amplification and first strand library preparation was undertaken using adapters for PCR with high fidelity polymerase and libraries were sequenced on the NextSeq 500 instrument (Illumina, San Diego, US) using either a 43bp or 81bp paired end run with general yield of ~15 million reads per sample.