GSM8409251: Sample_7_Microglia_Xenon 12%; Mus musculus; RNA-Seq
The microglia cells were isolated by cell sorter, and 1000 cells have their RNA extracted by Broad Institute using TCL buffer containing 1% Beta-Mercaptoethanol. Smart-Seq2 libraries were prepared by the Broad Technology Labs and sequenced by the Broad Genomics Platform. cDNA libraries were generated from sorted cells using the Smart-seq2 protocol65. RNA sequencing was performed using Illumina NextSeq500 using a High Output v2 kit to generate 2× 25bp reads.