AHE Illumina Sequencing
Capture, library preparation, and Illumina sequencing were performed at RAPiD Genomics, USA. Genomic DNA was sheared to 400 bp fragments, end-repaired and adenine residues were ligated to the 3-end of each blunt-end. Subsequently, barcoded adapters were ligated to the library and amplified by PCR. SureSelectxt Target Enrichment System for Illumina paired-end Multiplexed Sequencing Library protocol was used for solution-based target enrichment of pooled libraries. Finally, 150-bp long paired-end reads were generated in an Illumina HiSeq 3000.