GSM8230884: dTAG-SPT5-HCT116, WT, RNA, Rep1; Homo sapiens; RNA-Seq
Total RNA was extracted from cells with Trizol (Thermo Fisher Scientific). Drosophila RNA from S2 cells were added to samples (1:50) for spike-in. NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) was used to select mRNA. RNA-seq library was prepared with xGen™ RNA Library Preparation Kit (IDT).