16S amplicon sequences of microbial biofilms from plastic particles
The 16S rRNA gene was amplified with the primer pair 27F and 1492R. Then, the quality was checked through polymerase chain reaction (PCR). The sequencing of the V3-V4 region of the 16S rRNA gene was performed with v3 chemistry on a MiSeq Illumina sequencing platform at the Competence Centre for Genomic Analysis (CCGA) Kiel, Germany after the PCR products were visually assessed using 1% gel electrophoresis. For amplicon sequencing, the amplification of the V3-V4 hypervariable region of the 16S rRNA gene was accomplished using primer pair 341F (50-CCTACGGGAGGCAGCAG-30; Muyzer et al., 1993) and 806R (50-GGACTACHVGGGTWTCTAAT-30; Caporaso et al., 2011)