Illumina HiSeq 4000 sequencing; Drug tolerant persister cells of EGFR-mutant cell line under kinase-inhibitor combination
Drug tolerant persister cells of EGFR-mutant cell line under kinase-inhibitor combination
Cells were harvested during treatment with erlotinib, osimertinib, trametinib and dasatinib, following re-growth after treatment cessation or from untreated control cells RNA was extracted using the PicoPure RNA isolation kit following the manufacturer's instruction Libraries were constructed with the Lexogen 3' UTR RNA-sequencing kits