DNA-Seq of Podocarpus nubigenus: leaf tissue: chloroplast genome hits only
Library construction, sequencing and adapter trimming of genomic DNA from P. nubigenus, P. salignus and P. parlatorei was performed by BGI Genomics (https://www.bgi.com). One g of high-molecular weight DNA was randomly sheared with a Covaris ultrasonicator. The fragmented DNAs were checked by gel electrophoresis, purified using the AxyPrep Mag PCR Clean Up Kit (Corning MAG-PCR-CL-50), end-repaired by incubation at 20C for 30 min in BGIs proprietary End Repair Mix, and re-purified using the same kit, followed by A-tailing. Illumina adaptors were ligated to A-tailed fragments, and incubated at 16C for 16h. Adapter-ligated fragments were then size-selected for 270bp-350bp, enriched through PCR amplification, and libraries were quality checked using Agilent Technologies 2100 Bioanalyzer and ABI StepOnePlus Real-Time PCR System. Libraries were sequenced on the Illumina HiSeq 4000 platform, to generate over 8 million 150 bp paired-end reads for each species.