GSM7297451: assembloid culture, stromal cells, rep 1; Mus musculus; RNA-Seq
Libraries were constructed using Single Cell 3' Library & Gel Bead Kit v3 and the A Chip Kit (10x genomics) according to the manufacturer's instructions. Briefly, cells were resuspended in the master mix and loaded together with partitioning oil and gel beads into the chip to generate the gel bead-in-emulsion (GEM). Library was performed according to the manufacter's instructions (Single Cell 3' v3 Gene Expression protocol, 10x Genomics)