Targeted-enrichment sequencing of human eukaryotic DNA viruses: heart tissue
The study explored eukaryotic DNA virome in human organs. DNA was extracted from post-mortem tissue samples with Qiagen Mini Kit. Extracted DNA was mechanically fragmented and libraries prepared with KAPA HyperPlus kit. Targeted enrichment by in-solution hybridization with biotinylated baits for 38 human DNA viruses was applied followed by sequencing with hiseq.