GSM8231396: GFP-LC3 oHSC_MID_28; Mus musculus; RNA-Seq
BM derived cells were isolated , crushed and filtered through 40µm nylon mesh. These cells were stained for surface markers and sorted . Single cells were individually sorted by fluorescence-activated cell sorting into 96-well PCR plates containing lysis buffer (10% Triton X-100, SUPERase-In RNase Inhibitor 20U/μl (Thermo Fisher Scientific) and DEPC-treated or Rnase-free H20), at 4°C. Individual scRNA-seq libraries were prepared using a modified smartseq2 protocol (PMID: 30050112, PMID: 24385147 and PMID: 34057731).