RNA-Seq of Rattus_norvegicus: adult female bladder
16S rRNA amplicon sequencing was performed using probes listed in Supplementary Table S2. Paired-end sequencing reads of 2 x 251 bp in length were pre-processed using the DADA2 v1.26.0 pipeline in R, including quality filtering and trimming, paired reads merging, and chimeras removing55. The number and percentage of reads that passed each step can be found in Supplementary Table S3. DADA2 was then used to assign the amplicon sequence variants (ASVs) to the processed reads, and taxonomy was assigned using the Silva database v138.1 to each sequence variant