GSM6732245: TTC642_RNA_DOX_SNF5pos_Rep1; Homo sapiens; RNA-Seq
RNA was extracted from frozen cell pellets in biological triplicate using Zymo Quick-RNA Miniprep Kit (Zymo cat# R1054). Total RNA was prepared by Novogene Inc. using the Illumina TruSeq mRNA v2 kit with an input of 500ng of RNA for each sample to produce unstranded RNA libraries following the manufacturer's protocol. Final RNA libraries were quantified using the Qubit High Sense Reagent kit and Agilent Tapestation HSD1000 tapes. Libraries were equimolar pooled and paired-end sequenced across three lanes of an Illumina HiSeq 4000.