GSM7018380: ChIP_442_H3K9me3_rescue_exp_r3; Mus musculus; ChIP-Seq
Native ChIP: Cells were collected by scraping and nuclei were isolated. Chromatin was digested with MNase. Native Drosophila chromatin was spiked in and mononucleosomal fragments were immunoprecipitated, followed by library preparation and sequencing. Crosslinked ChIP: Cells were crosslinked with formaldehyde for 10 min and collected by scraping. Nuclei were isolated and chromatin was solubilized using a Covaris sonicator. Crosslinked Drosophila chromatin was spiked in. Libraries were prepared using standard Illumina protocols (KAPA Hyper Prep kit).