RNA-Seq of diadema antillarum: adult esophagus
Total RNA was isolated from portions of the whole esophagus dissected from An #3.  Trizol reagent (Invitrogen) was used to isolate the RNA followed by treatment with RNase-free DNase I (Qiagen), and then subsequent precipitation and reconstitution into nuclease free water.  Sample quality and quantity was assessed on an Implen NanoPhotometer spectrophotometer prior to shipment for sequencing at Psomagen (Macrogen USA) in Rockville, MD, USA.