GSM7718919: CD4 T cells GEX_HTO_Library; Mus musculus; RNA-Seq
Balb/c kidneys were transplanted into Il21CreRosa26Lox-STOP-Lox-YFP (IL21Fate Mapper) mice. 20 days post-transplantation, Tconventional (CD4+CXCR5-YFP-, LNTcon), Tfh21 (CD4+CXCR5+YFP+, LNTfh21) from dLN and graft infiltrating IL-21 producing cells (CD4+YFP+, Graft21) cells were sorted Cells were resuspended in 0.4% BSA in PBS at a concentration of 2,000 cells per μl, then loaded onto a single lane (Chromium chip K, 10X Genomics) followed by encapsulation in a lipid droplet (Single Cell 5′kit V2, 10X Genomics) followed by cDNA and library generation according to the manufacturer's protocol. 5' mRNA library was sequenced to an average of 50,000 reads per cell, V(D)J library and HTO (Cell Hashing antibodies) library sequenced to an average of 5,000 reads per cell, all using Illumina Novaseq.