GSM6383313: K562 ATAC HS rep1; Homo sapiens; ATAC-seq
Fresh cells were subjected to ATAC library construction protocol ATAC-seq was done using the original protocol and buffer {Buenrostro et al., 2013, #304248}. Incubation with Tn5 transposase was done in a shaking heating block at 37°C and 500 RPM for 30 minutes followed by real-time PCR amplification with Nextera dual index primers and NEBNext High-Fidelity 2x PCR mix with added Evagreen dye to avoid overamplification.