Illumina HiSeq 4000 sequencing; 3' UTR RNA-seq of EGFR-mutant PC9 cells and drug-resistant clones
3' UTR RNA-seq of EGFR-mutant PC9 cells and drug-resistant clones
Cells were harvested after resistant clones had been grown successfully Total RNA was extracted using Qiagen RNeasy kits, followed by DNAse digest Libraries were constructed with Lexogen 3' UTR RNA-seq kits according to manufacturer's instruction