RNA-seq of human Notch1-induced leukaemia cells derived from cord blood, which were transduced with wild type (wt) intracellular Notch1 (ICN1) lentivirus and in vitro treated with gamma-secretase inhibitor (GSI) up to 4 days before RNA isolation and sequencing.
Cells were sorted in PBS plus 3% FBS, centrifuged at 300g for 5 minutes, washed with PBS (Gibco) and centrifuged again at 300g for 5 minutes. PBS was aspirated and cell pellets frozen at -80C. Later, frozen cell pellets were delivered to the Michael Smith Genome Sciences Centre for RNA-seq processing. RNA was extracted and ribodepleted prior to sequencing. Paired-end 75 base pairs sequencing was performed.