GSM7018197: Escherichia coli MG1655 NCM3416 + tobramycin, biol rep 1; Escherichia coli str. K-12 substr. MG1655; RNA-Seq
Cultures were centrifuged, and RNA was purified using using the RNAeasy kit (Thermofischer Scientific), according to the manufacturer instruction (protocole 2 and 4). Illumina TruSeq mRNA Sample Prep Kit (20020595) was used with 7 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols (Krin et al., 2018, PMID: 29783948)