circRNA seq of duck: adult male breast muscle
Total RNA was extracted from pectoralis major muscle tissue using the Trizol method, then digested with RNase R to remove linear RNA, and purified using the RNeasy MinElite Cleanup kit. Perform specific chain library construction and sequencing according to the instructions of the VAHTS Total RNA seq (H/M/R) Library Prep kit (Novozan, Nanjing, China).