Transcriptome analysis on bovine oocytes after maturation supplemented with mimic negative control
The experimental design encompassed seven distinct groups: bta-novel-miR-906 mimic and inhibitor, bta-novel-miR-589 mimic and inhibitor, NC mimic and inhibitor, along with a control group devoid of any supplementation. For each group, four biological replicates were included, resulting in a total of 28 samples. Each sample underwent mRNA enrichment and purification using OligodT Selection, followed by RNA fragmentation and cDNA synthesis. Paired-end 100 bp sequencing was performed by BGI using the DNBSEQ-500 platform, yielding an average of 6.67 Gbp per sample.