GSM6733211: Evi5_MO_1; Xenopus laevis; RNA-Seq
Tadpole were anesthetized with 0.02% MS-222 and collect 10 limb buds in a group. Total RNA was extracted from the cells using TRIzol® Reagent (Invitrogen) and genomic DNA was removed using DNase I (TaKara) RNA libraries were prepared for sequencing using standard Illumina protocols