GSM6632430: HEK293T_BPK25_48h_BS_Seq_rep2; Homo sapiens; Bisulfite-Seq
The genomic DNA was extracted with Blood & Cell Culture DNA Kits (Thermo Fisher, Cat. #13323). Concentration of the DNA was measured with NanoDrop 2000 Spectrophotometers (Thermo Fisher). To prepare the bisulfite-seq sample, 5 μg of genomic DNA was sonicated into 250-550bp fragments with Covaris M220 ultrasonicator with the manufacturer's instructed setting. The fragmented DNA was directly applied for illumina Tru-seq library preparation. The library was quantified with Qubit and sequenced with illumina Hiseq 4000 sequencer with at least 20% Spike-in PhiX.