miRNA-seq revealing flowering mechanism in Rhododendron pulchrum
Total RNAs were isolated. The purity and concentration of RNAs were checked. Three biological replicates were performed for each sample. Small RNA libraries were constructed with TruSeq Small RNASample prep Kit. After PCR amplification, the enriched libraries were added with sequencing adaptors. Then, the libraries were purified, and checked. Quantitative analysis were performed. Sequences were carried out.