RNA-Seq of HeLa cells
Transcriptomic changes in HuR knockout (KO) and wild-type (WT) Hela cells exposed to Eltrombopag were investigated. DMSO was used as the Mock and  Eltrombopag (20uM) was used for treatment condition. For transcriptomic analysis, cells were exposed to the respective treatments for 48 hours and prepared in duplicates. Cells were seeded in 6-well plates and treated with DMSO (control) and Eltrombopag (20uM). After 48hrs, total RNA was extracted from two replicates per treatment using TRIzol reagent. RNA concentration was quantified using Qubit 2.0 Fluorometer. The samples with RNA Integrity Number (RIN)  9 were subjected to the subsequent analysis. A total of 2 g of total RNA per sample was used as input material for the RNA library. Enrichment of mRNA was performed with PolyA selection.  Sequencing libraries were generated using Standard RNA Library Prep Set for Illumina  following the manufacturers recommendations. Samples were sequenced on illumina NovaSeq 6000 to generate paired ended reads (150 bp). Libraries were constructed based on illumine True seq stranded primers.