GSM7666392: Jurkat T cells 1; Homo sapiens; Hi-C
Briefly, after the completion crosslinking and lysis, chromatin was digested with 200U DPN II restriction enzyme (Qiagen) at 37 °C. DNA fragments were purified and dissolved in water. Unligated ends were then removed. Purified DNA was fragmented to a size of 350-500 bp, and DNA ends were then repaired. DNA fragments labeled by biotin were finally separated on Dynabeads® M-280 Streptavidin (Life Technologies).