Sweet potato virus 2 isolate SPV2-LN, complete genome
The partial sequence of SPV2-Q isolate was obtained by small RNA sequencing. Primers SPV2-A-F/R, SPV2-B-F/R, SPV2-C-F/R, SPV2-1F/SPV2-1530R, SPV2-7103F/R, SPV2-10147F/Oligo(dT)18 were designed to amplify and eliminate the whole sequence of 5'-UTR. Finally, 5'RACE primers SPV2-243R and SPV2-136R were designed, and the 5'-UTR sequence was amplified by 5'RACE experiment with primers QT, Q0 and QI. All PCR products were sequenced and spliced to obtain SPV2-Q whole genome sequence.