RNA-seq of Plasmodium falciparum: 72 hpi drugged, rep 1
Total RNA was extracted with TRIzol LS Reagent (Invitrogen) followed by incubation for 1 hr. with 4 units of DNase I (NEB)at 37C. mRNAs were then purified using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) according to the manufacturers instructions. Libraries were prepared using NEBNext Ultra Directional RNA Library Prep Kit (NEB) and amplified by PCR with KAPA HiFi HotStart Ready Mix (KAPA Biosystems). PCR conditions consisted of 15 min at 37C followed by 12 cycles of [98C (30 s), 55C (10 s) and (62C 1 min 15)] and 5 min at 62C.