Adenoviruses in cloacal swab sample of a rosy-faced lovebird (Agapornis roseicollis)
Cloacal swab was taken and preserved in UTM Universal Transport Media (Copan, USA). Viral DNA was extracted from the UTM media by using 200l of the buffer and the High Pure Viral Nucleic Acid Kit (Roche Diagnostics, USA). To enrich for circular DNA, 2l of extracted viral DNA from each sample was used for rolling-circle amplification (RCA) using a TempliPhi Kit (GE Healthcare, USA). The RCA products were used to generate Illumina sequiecning libraries using the Illumina DNA Prep kit.