16S rRNA gene based amplicon sequencing of riverine biofilm microbial community
The microbial community diversity and structure were profiled using high-throughput amplicon sequencing of the V3-V4 region of the 16S rRNA gene. DNA extracts were sent to the IKMB Kiel University (Germany), and the partial 16S rRNA genes were sequenced on an Illumina Novaseq using the primers (v3f: CCTACGGGAGGCAGCAG; v4r: GGACTACHVGGGTWTCTAAT;).