GSM7845284: IP_GhD53_1; Gossypium hirsutum; OTHER
The gene sequence of the transcription factor is inserted into a vector containing an affinity tag to construct a protein expression vector for in vitro protein expression. The expressed transcription factor will carry the affinity tag. Genomic DNA is extracted from the sample to construct a DNA library, and then the in vitro expressed transcription factor with the affinity tag and the DNA library are combined in vitro. Subsequently, all paired DNA-protein complexes are separated out. Genomic DNA is extracted from the sample to construct a DNA library DAP-seq (DNA affinity purification sequencing) is a high-throughput method for discovering TF binding sites by using in vitro expressed TFs to probe genomic DNA. The DAP-seq dataset can also provide insights into the biology and binding site architecture of many TFs. It is a high-throughput detection method that uses in vitro expressed TF to interrogate bare gdna fragments in order to establish binding locations (peaks) and sequence motifs.