Microbial community structure in mycorrhizosphere soil of a former uranium mining area
Total soil genomic DNA was extracted from 0.25 g of soil sample using PowerSoil DNA Isolation kit (MoBio, Carlsbad, USA) according to manufacturers instructions in triplicates. Triplicates of each sample were pooled and sequenced using the Illumina MiSeq platform at StarSEQ (Mainz, Germany) after amplification of 16S rDNA region (bacteria) using primers 27F (5 - AGA GTT TGA TCC TGG CTC AG - 3) and 534R (5 - ATT ACC GCG GCT GCT GG - 3), and ITS1 region (fungi) using primers ITS1F (5 - CTT GGT CAT TTA GAG GAA GTA A - 3) and ITS2 (5 - GCT GCG TTC TTC ATC GAT GC - 3).