GSM8394783: WT_HS_RedC_biol_rep1; Escherichia coli; OTHER
Cells were fixed with 3% formaldehyde followed by treatment with lysozyme, SDS, and Triton X-100. DNA was digested with NlaIII. Downstream steps included RNA-to-DNA bridge ligation, reverse transcription, and library construction. Red-C uses custom oligos and adapters resulting in libraries ready for sequencing on the Illumina platform.