S. lomentaria; lineage1
Prior the genome DNA extraction, the gametophytes were cultivated with antibiotics (Penicillin G 50 mg/L, Ampicillin 50 mg/L, Chloramphenicol 5 mg/L, and Kanamycin 0.5g/L) for one week to remove eventual bacterial contamination. The gametophytes were frozen in liquid nitrogen and crushed to powder using TissueLyser II (QIAGEN) and genomic DNA was extracted using OmniPrepTM for Plant (GBiosciences).