scRNA-seq, V(D)J seq, CITE-seq, LIBRA-seq of human CD4+, CD8+ and B lymphocytes PBMCs of 5 donors (3 SARS-CoV-2 experienced and 2 SARS-Cov-2 Naive) before and following two doses of Pfizer/BioNTech BNT162b2 mRNA vaccine
scRNA-seq, V(D)J seq, CITE-seq, LIBRA-seq of human CD4+, CD8+ and B lymphocytes PBMCs of 5 donors (3 SARS-CoV-2 experienced and 2 SARS-Cov-2 Naive) before and following two doses of Pfizer/BioNTech BNT162b2 mRNA vaccine
Peripheral Blood Mononuclear Cells (PBMCs) were isolated by density-gradient centrifugation using Ficoll-Plaque protocol and cryopreserved for subsequent cell sorting and scRNA-Seq analyses.  Single cell suspension was used according to Chromium Next GEM Single cell V(D)J Reagent kits v1.1 Chromium Next GEM Single Cell 5' Library & Gel Bead Kit v1.1, 16 rxns PN-1000165 Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120 Single Index Kit T Set A, 96 rxns PN-1000213 Chromium Next GEM Single Cell 5' Library & Gel Bead Kit v1.1, 16 rxns PN-1000165 Chromium Single Cell 5' Library Construction Kit, 16 rxns PN-1000020 Chromium Single Cell V(D)J Enrichment Kit, Human T Cell, 96 rxns PN-1000005 Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120 Single Index Kit T Set A, 96 rxns PN-1000213  Chromium Next GEM Single Cell 5' Library & Gel Bead Kit v1.1, 16 rxns PN-1000165 Chromium Single Cell 5' Library Construction Kit, 16 rxns PN-1000020 Chromium Single Cell V(D)J Enrichment Kit, Human B Cell, 96 rxns PN-1000016 Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120 Single Index Kit T Set A, 96 rxns PN-1000213 Chromium Next GEM Single Cell 5' Library & Gel Bead Kit v1.1, 16 rxns PN-1000165 Chromium Single Cell 5' Library Construction Kit, 16 rxns PN-1000020 Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120 Chromium Single Cell 5' Feature Barcode Library Kit, 16 rxns PN-1000080 Single Index Kit N Set A, 96 rxns PN-1000212