RNAseq of Rhodococcus aetherivorans strain L13 CO2--grown cells
Total RNA was extracted as described by LeBlanc et al. (2008) and purified using Easypure RNA kit (TransGen Biotech, Beijing, China). Quantification was performed by using the QubitRNA HS Assay kit (Invitrogen, Life technologies, California, USA). Expression analyses were performed by Macrogen Inc. (Seoul, South Korea) on two biologically independent experiments. Briefly, the total RNA samples were processed using the NEB-Microbe Ribo-Zero kit to remove rRNA. The resulting mRNA (1 ng) was used to construct libraries with a TruSeq Stranded total RNA kit (New England Biolabs, Ipswich, USA), and sequenced using NovaSeq 6000 S4 Reagent Kit (Illumina Inc., San Diego, USA).