GSM6595713: MTA2_ChIP in Satb2KO colonic epithelium rep 2; Mus musculus; ChIP-Seq
50 µl of pelleted cross-linked cells were resuspended in 350 µl sarkosyl lysis buffer (0.25% sarkosyl, 1 mM DTT and protease inhibitor in RIPA buffer) and sonicated at 15% amplification by a tip sonicator (Qsonica, Q125) to obtain 200bp to 800bp chromatin fragments. Diluted lysates were incubated with TFs antibodies at 4°C overnight and were additionally incubated with 30 µl protein A/G magnetic beads. ChIPed DNA was purified with a MinElute purification kit. Libraries were prepared using the ThruPLEX DNA-Seq Kit Exactly followed the protocol from Takara bio, R400428. All final libraries were size selected (200-800 bp) by AMPure XP beads (Beckman, A63880) and loaded for sequencing.