RNA-seq of overwatered ginseng for 3week, rep3
P. ginseng seedlings (Yunpoong, provided by National Institute of Horticultural and Herbal Science) were grown at 22 C with a long day with the ginseng cultivation soil medium in green house. For ambient water stress treatment, soil moisture contents were kept in 100 % with automated watering system. Plant samples were harvested and freeze with liquid nitrogen immediately till Total RNA extraction was performed. Total RNAs were extracted from ginseng samples using Easy Spin RNA Extraction Kit (iNtRON Biotechnology) with the manufacturer's instructions. Their quality and quantity were examined using a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Two-microgram total RNAs were used to construct RNA-Seq libraries using an Illumina TruSeq RNA Sample Prep Kit according to the manufacturers instructions. Pooled libraries were sequenced using an Illumina HiSeq X platform with paired-end (PE) reads of 151 bp.