Dec.cathode ECOFB-MES_2.raw
The total DNA contents of different microbial samples were extracted after pretreatment using the E.Z.N.ATM Mag-Bind Soil DNA Kit (Omega Bio-Tek, USA) according to the manufacturers instructions. The primer pairs 341F (5-CCTACGGGNGGCWGCAG-3) and 805R (5-GACTACHVGGGTATCTAATCC-3) were used to amplify the hypervariable V3-V4 region of the 16S rRNA gene.The PCR products after two amplification per sample were gel-purified. Finally, libraries based on the pooled PCR reactions were sequenced on the Illumina Miseq 2  300 bp platform.