Short read sequencing of C. elegans wild isolates
Genomic DNA  was prepared using standard  phenol/chloroform extraction and ethanol precipitation. Samples were cleaned with DNA Clean  & Concentrator columns (Zymo Research #D4004). Libraries were prepared using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs #E7805) and Multiplex Oligos for Illumina (NEB #E7500), with customized fragmentation and purification steps to enrich for desired sizes. A final DNA size selection targeting 650bp50bp was performed using BluePippin (Sage Science). The libraries were sequenced on a HiSeq 2500 (Illumina) on Rapid Run Mode (paired-end 2x250bp) in the Molecular Evolution Core at the Georgia Institute of Technology.