WGS of NCI-PC35-1 organoid
DNA was extracted from saliva using the Genotek prepIT DNA kit. 1ug of DNA was sheared and assembled into whole genome sequencing libraries using the Illumina TruSeq DNA Library Preparation Kit. Samples were sequenced on the Illumina platform. Pass-filter FASTQ files were trimmed using Trimmomatic, aligned with BWA-MEM, duplicate-marked and quality-score recalibrated using GATK version 4.