GSM8230313: Wild type 2; Solanum lycopersicum; miRNA-Seq
Total RNA was extracted from terminal leaflets (30 days post germination) and fruits (mature green and red ripe) of wild-type and transgenic plants using TRIzol reagent (Invitrogen, Cat #15596026) according to the manufacturer's instructions. Briefly, 3' and 5' adaptors were ligated to 3' and 5' ends of small RNA, respectively. Then the first strand cDNA was synthesized after hybridization with reverse transcription primer. The double-stranded cDNA library was generated through PCR enrichment. After purification and size selection, libraries with insertions between 18~40 bp were ready for sequencing on Illumina sequencing platform with SE50.