VvAGL12 all tissues for 10 Days
After the sample is qualified, the library construction is carried out. The main process is as follows:(1) Enrich eukaryotic mRNA with magnetic beads with Oligo (dT);(2) Add Fragmentation Buffer to randomly interrupt mRNA;(3) Using mRNA as a template, use random hexamers to synthesize the first cDNA strand, then add buffer, dNTPs, RNase H and DNA polymerase I to synthesize the second cDNA strand, and use AMPure XP beads to purify cDNA;(4) The purified double-stranded cDNA is then subjected to end repair, A tail is added, and the sequencing adapter is connected, and then AMPure XP beads are used for fragment size selection;(21) Finally, the cDNA library was obtained by PCR enrichment.