RNA-Seq of sus scrofa: adult male liver
mRNA was enriched by Oligo(dT) beads, fragmented into short fragments, and reversly transcribed into cDNA by using NEBNext Ultra RNA Library Prep Kit for Illumina.The purified double-stranded cDNA fragments were end repaired, A base added, and ligated to Illumina sequencing adapters.The ligation reaction was purified with the AMPure XP Beads(1.0X).And polymerase chain reaction (PCR) amplified.