RNA-seq of human: PBMCs 3
RNA samples were extracted using TRIzol Reagent (Thermo Fisher) per manufacturer instructions. mRNA was isolated using NEBNext Poly(A) mRNA Magnetic Isolation Module. cDNA libraries were prepared according to the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina protocol. Paired-end sequencing was performed on a NovaSeq 6000 platform (Illumina).