RNA-Seq of homo sapiens: A549 cell line
After the RNA extraction and quality control, RNA-seq transcriptome libraries were prepared using 1 ug of total RNA according to the TruSeqTM RNA sample preparation kit. Sequence was performed using Illumina NovaSeq 6000 with 2150 bp read length. Trimming and quality control of raw end-of-pair reads by SeqPrep and Sickle. The clean reads were then individually mapped to the reference genome using the HISAT2 software. Mapped reads for each sample were generated by StringTie.