ddRAD-seq on tomato: E17 genotype
Total genomic DNA was extracted from 100 mg of young leaf tissue from genotypes using the DNeasy plant mini kit (Qiagen, Hilden, Germany). For DNA sequencing, samples were used to prepare libraries for the ddRAD sequencing, as described in Peterson et al. 2012, PLoS ONE 7, e37135 with minor modifications. MboI and SphI enzymes were used for restriction digestion and fragments sequenced with the V4 chemistry paired end 125 bp mode on the HiSeq2500 Illumina instrument.