Q0115_PPC_C5P26_PPC023_scRNA_R01L01S01
Detailed methods are found in PMID 37903777. All single cells were captured using the 10X Chromium controller (10X Genomics) according to the manufacturer's specifications and manual (Manual CG000183, Rev A). Cells from each scRNA-seq sample were loaded each onto an individual lane of a Chromium Single Cell Chip B. Libraries were generated using Chromium Single Cell 3' Library Gel Bead Kit v3 (10X Genomics) following manufacturer's manual with small modifications. Specifically, the purified cDNA was eluted in 24ul of Buffer EB, half of which was used for the subsequent step of the library construction. cDNA was amplified for 10 cycles and libraries were amplified for 8 cycles. All libraries were sequenced on a HiSeq 4000 using custom programs.