Micobacterium sp SLF095 isolated from Serpula lacrymans
Genomic DNA was extracted from bacteria grown on TSA plates (not older than 4 days) using the Dneasy PowerLyzer PowerSoil Kit (Qiagen, The Netherlands) according to the manufacturer's instructions with noted alterations. Samples were homogenized twice using FastPrep-24 5G (MP Biomedicals, Santa Anna, USA) at 6 m/s for 30 s (2 cycles, 300 sec pause). In the last step, DNA was eluted from the columns using 100 ul of solution C6 (10 mM Tris). The extracted DNA was checked for quality using 1% w/v agarose gel electrophoresis (100V, about 20 min), quantified with a DeNovix DS-11 spectrophotometer (DeNovix Inc., Wilmington, DE, USA), and stored at -20C until further use. Microbial whole genome sequencing was carried by Novogene Co., Ltd. (Beijing, China). Library preparation was conducted by the company and included fragmentation of the genomic DNA (size of 350 bp). The selected fragments were then end-polished, A-tailed, and ligated with the full-length adapter. Sequencing was conducted using the Illumina NovaSeq platform (NovaSeq PE150, Q30  85%, 1 G raw data per sample).