GSM7719389: ACH_2; Homo sapiens; RNA-Seq
Briefly, the latent T-cells ACH2, J1.1, and J-Lat were revived in RPMI-1640 (HyClone; Cat# SH30096.01) supplemented with 2 mM L-glutamine (Gibco; Cat# 25030-081), 100 U/ml penicillin, 100 µg/ml streptomycin (Gibco; Cat# 15140-122), 10% fetal bovine serum (Atlas Biologicals; Cat# F-0500-A) and 10 mM HEPES (Sigma; Cat# H0887). After 2 passages, the cells were split in the respective medium 1 million cells/ml and then cells were submitted to Einstein Genomics facility for scRNA-Seq. Single-cell mRNA libraries were built using the Chromium Next GEM Single Cell 3' Library Construction V3 Kit according to the manufacture's protocol.