GSM8393377: whole animal, control strain exposed to DMSO, replicate 1; Caenorhabditis elegans; OTHER
Genomic DNA was extracted with QIAGEN genomic-tips (20/G) following the manufacturer's protocol. The DNA quantity was measured using the Qubit 2.0 (Thermo Fisher) coupled with Qubit dsDNA HS assay kit (Thermo Fisher). The quality was assessed by gel electrophoresis loading 150 ng of sample into each well and separated for 40 minutes at 100 V. Whole genome resequencing was based on  SBS (sequencing by synthesis) to identify the genetic variations in individual strains . The genomic DNA was randomly sheared into short fragments. The obtained fragments were end repaired, A-tailed and further ligated with Illumina adapter. The fragments with adapters were PCR amplified, size selected, and purified.The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries  were sequenced on the Illumina platform, according to the library concentration. Whole Genome Sequencing Illumina NovaSeq PE150