GSM7767257: iALI-Control-_scRNA; Homo sapiens; RNA-Seq
Cells were dissociated from Transwells by using Trypsin (15 minutes, 37°C). Then, cells were passed into a  40 μm Corning filter and centrifuged at 1200 rpm for 5 min. All cells were maintained in PneumaCult™-ALI Medium and separated in 2 ependorfs to perform isotype-PE and CD326 antibody labelling (30 min at 4°C). At last, after several washes with media complemented with BSA (0,01%), cells were sorted on BD FACSAria™, with MRI platform. At last, 10 000 cells were captured for single cell RNA sequencing in each condition. Libraries were constructed according to the manufacturer's instructions using Chromium™ Single Cell 3' v3.1 Reagent Kits and a Chromium instrument.