RNA-seq of soybean
Soybean roots were harvested at 12 h, 24 h, 72 h and 8 d after B deficiency or control treatment for mRNA library construction and sequencing. Total RNA was isolated using the Trizol Reagent (Invitrogen Life Technologies), after which the concentration, quality and integrity were determined using a NanoDrop spectrophotometer (Thermo Scientific). Three micrograms of RNA were used for the RNA sample preparations. Then the Sequencing libraries were generated and sequenced on NovaSeq 6022 platform (Illumina) by Shanghai Personal Biotechnology Cp. Ltd.