GSM8279257: LFH2, replicated 2, scRNAseq; Homo sapiens; RNA-Seq
Human patients diasgnosed with LSCS accompanied by LF hypertrohpy were collected during elective surgery involving decompressive lumbar laminectormy. LF tissues acquired during surgery were rinsed thoroughly with saline solution an dplaced in a conical tube containing MACS Tissue Storage Solution. The tissue was then transferred to the laboratory for tissue dissociation. The LF tissue was rinsed then dissociated with Hank's balanaced saline solution mixed with 1% penicilin/streptomycin. After remiving bold debris and blood, specimens were minced with a blade on a petri dish, and series of enzymatic digestion reactions were performed using Ham's F-12 medium containing 1% P/S, 5% heat-inactivated fetal bovine serum, and 0.2% protease followed by rinsing. A 70-μm strainer was used to separate the cells from tissue debris after enzymatic digestion. After filtration, the solution with the isolated cells was centrifuged. The remaining cells were counted with a cell counter and stored in a conical tube. Library was performed according to the manufacter's instructions (single cell 3' v3 chemistry, 10x Genomics).