GSM8185740: Postnatal (P6) whole ventricle, rep1; Mus musculus; RNA-Seq
Total RNA was extracted using the QIAzol Lysis Reagent (79306, QIAGEN) and miRNeasy Micro Kit (217084, QIAGEN) followed by digestion of DNA using the TURBO DNA-free™ Kit (AM1907, Invitrogen). Isolated RNA quality was assessed using an Agilent 2200 TapeStation system (Agilent). 1 µg of RNA with a RIN e score of 7 or higher was used for preparation of sequencing libraries with Poly(A) Magnetic Isolation Module (E7490S, New England Bio Labs) and NEBNext Ultra RNA Prep Kit for Illumina (E7530S, New England Bio Labs).