GSM7299858: ChIL_2PN_2; Mus musculus; OTHER
BDF1(C57BL/6×DBA2) ×JF1/Ms zygotes were selected 10 h after fertilization (late G2 or PN5 stage) and the second polar body was removed using a micromanipulator. Zygotes were fixed with 1% PFA for 30 min after removal of the second polar body. After permeabilization with 1% Triton X-100 for 10 min, zygotes were blocked with Blocking One-P (Nacalai Tesque) for 20 min. Zygotes were incubated with mouse anti-histone H3K4me3 antibody (1:200, Cosmo Bio, MABI0004-100) for 8-10 h at 4°C. We observed the immunofluorescence signals of the probes under a microscope to selectively collect 1PN biparental zygotes and not parthenotes. ChIL-seq libraries were prepared as previously described 45 with some modifications, including a change in the SDS concentration from 0.2% to 0.05%.