GSM7874459: scAIR001: scATAC-seq and interactome; Drosophila melanogaster; OTHER
Crosslinked cells were initially subjected to cell lysis and nuclei lysis to extract the nuclei. The lysed nuclei were then fragmented into smaller complexes using restriction enzymes, which cut the DNA at specific recognition sites. Following fragmentation, proximity ligation was performed to join the fragmented DNA ends together. This step helps generate smaller DNA fragments with proximity ligation, allowing for easier sequencing. The ligated nuclei were subsequently uploaded to the 10x Genomics platform for further processing and analysis. The barcoded RNA and DNA by 10x genomics were performed library construction following the manual.