GSM7718888: cardiac fibroblasts from Smad3 fl/fl db/db animals, 3; Mus musculus; RNA-Seq
RNA was extracted with Trizol reagent. RNA sequencing was performed by Novogene. RNA libraries were prepared for sequencing using standard Illumina protocols RNA-seq. Library preparation was performed by Novogene, Inc., using at least 1 ug high-quality total RNA input per sample. mRNA enrichment was performed with poly-T oligo-attached magnetic beads. Libraries were constructed using NEBNext Ultra RNA Library Prep Kit for Illumina.