16s rRNA of water
The hypervariable V3 and V4 regions of the bacterial 16S rRNA gene were amplified using the primers 341F (5CCTAYGGGRBG-CASCAG-3) and 805R (5GACTACHVGGGTATC-TAATCC-3) (Klindworth et al. 2013), prior to sequencing on the Illumina MiSeq platform (Ramaciotti Centre for Genomics (Sydney, NSW, Australia))