GSM7665528: HC,scATAC-seq; Homo sapiens; ATAC-seq
CD45+CD19+ B cells were sorted using SH800S Cell Sorter (SH800S, SONY). Samples were lysed in cold lysis buffer (10 mM Tris-HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl2, and 0.1% NP-40(Roche)) for 3 min on ice to prepare the nuclei. Immediately after cell lysis, nuclei were centrifuged at 500 g for 5 min, and the supernatant was discarded carefully. Nuclei extracts were then incubated with Tn5 transposase (cat no. TD501, Vazyme Biotech) at 37℃ for 30 min. After DNA purification with the MinElute Kit (Qiagen), PCR was performed to amplify the library for 12–15 cycles based on the quantitative data regarding the optimum number of PCR cycles. The PCR conditions were as follows: 98℃ for 30 sec and cycling at 98℃ for 10 sec, 63℃ for 30 sec, and 72℃ for 1 min. After PCR amplification, the sample libraries were purified and sequenced on the MGISEQ-2000 platform with the 150-bp paired-end configuration.