1CSC28_A5OE_merip seq_input_CUHKIDD_A5
Total RNAs were extracted with Trizol reagent (Thermo Fisher Scientific) and DNA contamination was removed by DNase I (Roche Diagnostics). The total RNA was chemically fragmented into approximately 200-nt-long fragments. The fragmented RNAs were then incubated with the Protein A/G Magnetic Beads (Thermo Fisher Scientific) and tumbled with anti-m6A antibody (Merck Millipore) at the presence of RNase inhibitor (Thermo Fisher Scientific). Then the eluted RNA was reverse transcribed with High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific). Then the sequencing was performed on an Illumina Novaseq 6000 platform.