Genome sequencing of Listeria monocytogenes phage isolated from food industry surfaces
To isolate bacteriophages from lysed plates, a sterile tip was used to collect areas of viral replication. The collected viral replication area was transferred to an Erlenmeyer flask containing 25 mL of BHI broth and 1 mL of bacterial culture in the exponential growth phase (OD 600nm= 0.6 to 0.9). The mixture was incubated in an oven at 37 C for 16 hours to propagate the isolated phages. Subsequently, the tubes were centrifuged at 10,000  g for 5 minutes at 4 C to decant the bacterial cells. The supernatant was filtered through cellulose acetate filters with 0.22 m pores to obtain purified viral particles and these were stored at 4 C. Library was prepared following Illumina DNA prep Kit, with (i) tagmentation (ii) indexing by PCR; (iii) size selection - remove short fragments and sequencing.