16S rRNA amplicon sequencing of a membrane-aerated biofilm: 60% O2 Dup1
Biofilms were scraped into PBS at reactor shutdown. Biomass samples were centrifuged at 12,500 g for 10 min and pellets were stored at 20 C until DNA extraction. DNA was extracted using the FastDNA SPIN Kit for Soil. The extracted DNA was PCR amplified using the universal primer set 338F and 806R, which amplifies the V3-V4 hypervariable region of the 16S rRNA gene. Purified PCR products were sequenced on the Illumina NovaSeq 6000 platform with paired-end reads of 2  250 bp length.