microbiome of preterm infant saliva, stool, and milk
DNA extractions and purifications were performed using the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit and automated on KingFisher Flex robots . Blank controls and mock communities were included per extraction plate, which were carried through all downstream processing steps. Input DNA was quantified using a PicoGreen fluorescence assay and metagenomic libraries were prepared with KAPA HyperPlus kits and automated onEpMotion automated liquid handlers. Sequencing was performed on the Illumina NovaSeq 6000 sequencing platform with paired-end 150bp cycles.