GSM8230911: N2 worms, day 7, embryo lysates rep1; Caenorhabditis elegans; RNA-Seq
Synchronized worms were collected from the plates, washed at least three times with M9 buffer, and resuspended in TransZol Up reagent (TransGen Biotech, #ET111-01). Worm samples were frozen in liquid nitrogen and freeze-thawed for at least five times. Total RNA was isolated by chloroform extraction followed by isopropanol precipitation, washed with 75% (v/v) ethanol and dissolved in RNase-free water. RNA libraries for RNA seq were prepared by BGI Tech using DNBSEQ mRNA seq protocols.