RNA-Seq of Malus domestica cv Gala transgenic line 2024 buds
The poly-A fracton was isolated from total RNA and fragmented. After fragmentation, the first strand cDNA was synthesized using random hexamer primers. Then the second strand cDNA was synthesized using dUTP, instead of dTTP. The directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification.