DNA-Seq of Podocarpus nubigenus: leaf tissue: chloroplast genome hits only
Library construction, sequencing and adapter trimming of genomic DNA from P. sellowii was performed at the Max Planck-Genome-Centre Cologne, Germany. Two hundred ng of genomic DNA were fragmented using a COVARIS S2 ultrasonicator and Illumina-compatible libraries were prepared using the NEBNext Ultra DNA v2 Library Prep Kit for Illumina (New England Biolabs) following the manufacturers protocol. Libraries were sequenced on a HiSeq 2500 device with the HiSeq Rapid PE Cluster Kit v2 and the HiSeq Rapid SBS Kit v2 (500 cycle) to generate ~2.2 million 250 bp paired-end reads.