GSM6696294: HAP1 WT #4 rep2; Homo sapiens; RNA-Seq
Total RNA was isolated using RNAzol (Sigma, cat#R4533-100ML) per manufacturer's instruction. RNA was quantified with Qubit RNA HS BR Assay Kit (Thermo Fisher Scientific, cat#Q10210??) and Qubit 4 Fluorimeter according to manufacturer's instruction. RNA integrity was verified with HS RNA Kit (15NT nt) on the 5200 Fragment Analyzer (Agilent Technologies, cat# DNF-472-500) per manufacturer's protocol. The ONT PCR-cDNA sequencing kit (Oxford Nanopore Technologies, cat# SQK PCS-109) was used for library preparation per manufacturer's instructions. For each sample, 50 ng of input total RNA was used. Libraries were sequenced on the MinION Mk1C using R9.4.1 flow cells (Oxford Nanopore Technologies, cat#??)