GSM8279322: HGC-27 cells, shControl rep2,10%FBS; Homo sapiens; RNA-Seq
Total RNA was extracted from the cell using TRIzol® Reagent according the manufacturer's instructions. RNA purification,reverse transcription,library construction and sequencig were performed at ShanghalMajorbio Bio-pharm Biotechnology Co., Ltd. (Shanghai, China) according to the manufacturer's instructions . The RNA-seq transcriptome librariy was prepared following llumina® Stranded mRNA Prep, Ligation (San Diego,CA) using 1μg of total RNA.