Human vaginal microbiota from sample C1 (status: Healthy Control)
Genomic DNA was isolated from vaginal cell pellets using a DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany). DNA concentration and quality were determined using a NanoDrop ND1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA) and a TapeStation 2200, (Agilent Technologies, Santa Clara, CA, USA). The V3V4 hypervariable regions of the 16S ribosomal RNA (rRNA) gene were amplified according to the 16S Metagenomic Sequencing Library Preparation protocol (Illumina, San Diego, CA, USA) and sequenced on a MiSeq platform (Illumina), in a single 2300 bp paired end run.