18S amplicon sequencing of lake Cadagno
Lake water was filtered on 0.2 m polycabonate filters. DNA was extracted with a hot phenol/chloroform method. The sequencing library was constructed with primers euk516f:GGAGGGCAAGTCTGGT & euk1055r:CGGCCATGCACCACC. Sequencing was done for 2x300 bp paired end reads. However, reads did not show enough overlap. Therefore, only single read data was uploaded