The WES sequencing data was cleaned to pass quality controls, and mapped to human reference genome (hg19/GRCh37) by Bowtie2 (V2.4.2) with no more than 2 mismatches. The mapped BAM file was sorted by SAMtools (V.1.11) and removed duplicates by Picard (V2.20.1). Control-FREEC (control-FREE Copy number caller) (V11.6) was used to identify Copy Number Variations (CNVs) based on default parameters.
The WES sequencing data was cleaned to pass quality controls, and mapped to human reference genome (hg19/GRCh37) by Bowtie2 (V2.4.2) with no more than 2 mismatches. The mapped BAM file was sorted by SAMtools (V.1.11) and removed duplicates by Picard (V2.20.1). Control-FREEC (control-FREE Copy number caller) (V11.6) was used to identify Copy Number Variations (CNVs) based on default parameters.