GSM7665719: MP-3_DMSO_4hrH2O; Mus musculus; OTHER
Cultures were lysed in Tri-reagent BD (Sigma, T3809) and frozen for future procedures. RNA was purified by phenol-chloroform extraction and isopropaonol precipitation, treated with DNase-I (NEB, M0303) then fragmented by high-magnesium, high-temperature incubation. One 1μg of Bru-containing  RNA was used for library preparation. The library was prepared in collaboration with the BrU-seq lab of the University of Michigan (Paulsen et al., 2013, 2014)