GSM6732182: CPT2KO-2; Homo sapiens; RNA-Seq
The cells were washed by DPBS and harvested by buffer RL. The HiPure Total RNA Mini Kit (Magen, Cat# R4111-03) was used to extract total RNA following the manufacturer's instructions and the RNA was quantitated with nanodrop 2000 (ThermoFisher). RNA libraries were prepared for sequencing using standard Illumina protocols.