GSM7874089: Ctrl keratinocytes rep 1, input; Mus musculus; OTHER
Briefly, fragmented RNA was incubated with anti-m6A polyclonal antibody (202003, Synaptic Systems, Germany) in IPP buffer for 2 hours at 4°C. The mixture was then immunoprecipitated by incubation with protein-A beads (Thermo Fisher) at 4°C for an additional 2 hours. PolyA mRNA was enriched using GeneElute mRNA Miniprep Kit. MeRIP-Seq was performed by Cloudseq Biotech Inc. (Shanghai, China) according to the published procedure with slight modifications.