GSM8183558: S5_blood_14_ivig; Mus musculus; RNA-Seq
Total RNA from bone marrow derived murine neutrophils and monocytes, or whole blood, from control and treated mice was purified using the RNeasy Plus Mini kit (QIAGEN, Germany) following the manufacturer's instructions. Library was prepared using a 250-300 bp insert cDNA library (low-input). mRNA was enriched using oligo (dT) beads and randomly fragmented in fragmentation buffer followed by cDNA synthesis using random hexamers and reverse transcriptase. After first-strand synthesis, a custom second-strand synthesis buffer (Illumina) was added with dNTPs, RNase H and Escherichia coli polymerase I to generate the second strand by nick-translation. The final cDNA library was ready after a round of purification, terminal repair, A-tailing, ligation of sequencing adapters, size selection, and PCR enrichment.