GSM7767647: HSAEC, UI-6; Homo sapiens; RNA-Seq
Total RNA was extracted from approximately 1 million of NHBE or HSAEC cells using Trizol (Invitrogen, Carlsbad, CA, USA) according to manufacturer's instructions. Ten μg of purified RNA from each sample was sent to Genewiz commercial sequencing facility (South Plainfield, NJ) for Bioanalyzer quality control analysis (Agilent, Santa Clara, CA) and Illumina Next Generation Sequencing. All the submitted total RNA samples had an RNA integrity number (RIN) of 10. RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.