GSM7749019: CD34, donor #1, scRNAseq; Homo sapiens; RNA-Seq
Peripheral blood NK cells were purified using the RosetteSep Human NK Cell Enrichment Cocktail (STEMCELL Technologies). CD34+ hematopoietic progenitor cells were enriched using the Indirect Human CD34 MicroBead Kit (Miltenyi Biotec). Following cell sorting, cells were stained for flow cytometry and CD34+ cells were further purified via sorting to >99.9% purity on an FACSAria II cell sorter (BD Biosciences). Human innate lymphoid cells were enriched from fresh tonsil tissue and blood samples  using a custom human NK cell enrichment RosetteSep reagent containing a mixture of bivalent antibodies against glycophorin A and CD3, CD4, CD19, CD20, CD36, CD66b, and CD123. Approximately, 10,000 cells were loaded on the 10X Genomics Chromium platform and libraries were constructed with a Chromium Next GEM Single Cell 5´ reagent kit v2 dual index according to the manufacturer instructions