RADseq of Cardamine serbica 22PERU9
Double-digest RADseq libraries were prepared according to the protocol of Peterson et al. (2012) with some modifications as described in detail in lenker et al. (submitted). Genomic DNA was digested with HpyCH4V (New England Biolabs), subjected to A-taling, followed by adaptor ligation with a 5-base pair long barcode. The pooled ligation products were size-selected with Pippin Prep (Sage Science, Inc., MA, USA) and PCR amplified using universal P5 primer and the indexed P7 primer (6-base pair-long indexes). The final ddRADseq library was sequenced with 150-bp paired-end reads on Illumina systems at Novogene, Cambridge, UK.