GSM6696328: CAGE_dcp2D_Replicate_1; Saccharomyces cerevisiae; RNA-Seq
CAGE libraries were constructed from 5µg of total RNA isolated from two biological replicates of each strain using the nAnT-iCAGE protocol (Murata et al. 2014) by DNAFORM in Yokohama, Japan. After first strand cDNAs were transcribed to the 5' end of capped RNAs, cap structure of mRNA was chemically biotinylated to allow streptavidin capture, RNase1 digested, captured using avidin magnetic beads and attached to CAGE “barcode” tags. Each CAGE library used linkers with specific barcodes and was sequenced using Illumina NextSeq 