Single cell RNA sequencing from post treatment PBMC of patient 1246
Single-cell complementary DNA libraries were prepared and sequenced at the University of Michigan Sequencing Core using the 10× Genomics Platform. Samples were run using 50-cycle paired-end reads on the NovaSeq 6000 (Illumina) to a depth of 100,000 reads.  Cell Ranger count version 6.0.0 was used with default settings, with an initial expected cell count of 10,000.  The hg19 reference supplied with the Cell Ranger software was used for alignment.