GSM7295217: cMyc-GFP+ LZ GC-B cells, KO4; Mus musculus; RNA-Seq
Spleens were crushed and filtered with a 70µM mesh strainer. Harvested splenic B-cells were enriched by CD43, Ter119 and CD4 negative selection using MACS LS Columns (Miltenyi Biotec). Single-cell suspensions were stained with antibodies and sorted by flow cytometry. RNA was extracted from the sorted samples by TRIzol coupled to RNAeasy Mini Kit (#74104, QIAGEN, for cMyc+ GC-B cells). Libraries were prepared with TruSeq Standard mRNA Sample Prep Kit (Illumina) following the manufacturer's protocols.