Methanosphaera sp. ISO3-F5 genome sequencing
Thirty micrograms of DNA was sent to Novogene Co., Ltd (Singapore) for library construction, quality control, and long-read sequencing by Pacific Biosciences (PacBio) Sequel II v8.0 system using single-molecule real-time SMRTbell libraries. The Sequel II DNA CLR library preparation kit v2.0 (PacBio) was used for library preparation according to the manufacturers protocol and sequenced using two SMRT Cells on the Sequel II platform with continuous long read (CLR) mode.