GSM7299817: P_27; Mus musculus; RNA-Seq
C57BL/6 × JF1/Ms zygotes were selected 10 h after fertilization (late G2 or PN5 stage) and the second polar body was removed using a micromanipulator. Two-cell embryos were corrected 34 h after fertilization. Single 2-cell embryo were lysed using cell lysis buffer  [cantained in SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio)] in 0.2 mL single tube. libraries were prepared with SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio) and Nextera XT DNA Library Preparation Kit (illumina).