Illumina NovaSeq 6000 paired end sequencing; scapis_uppsala_1973 Fecal DNA
Sequencing of fecal DNA for mapping to the human gut microbiome
Fecal DNA was extracted using NucleoSpin® 96 Soil kit (740787; Macherey-Nagel; Germany). Samples were subjected to 5 min of bead beating at 2200 rpm, with 1.2 μg of DNA obtained on average per sample. The sequencing of metagenomes was performed using 2 x 150 bp paired-end sequencing on Illumina Novaseq 6000 system (Illumina, USA).