GSM8279313: Control3; Homo sapiens; RNA-Seq
For the QC of purified RNA, absorbance ratios were assessed with NanoDrop 2000 spectrophotometer Total RNA quality was evaluated based on the RNA integrity number (RIN) using a BioAnalyzer 2100 and quantified with a Qubit 4 Fluorometer. Next, RNA-Seq libraries were prepared using the NEB Next Ultra II RNA Library Prep Kit Quantifying the pooled libraries required for RNA-seq was determined by real-time qPCR using a KAPA's library quantification kit for the Illumina sequencing platforms. Pooled libraries were sequenced using the Illumina NextSeq 500/550 System with a Paired-end Mid Output v2 kit, 150 cycles.