GSM6383010: H430_RC_S42; Mus musculus; RNA-Seq
Trizol reagent was added to harvested sciatic nerve and RNA was prepared according to manufacturer's protocol (Invitrogen cat#15596018). RNA samples were reverse transcribed on Applied Biosystems MiniAmp Thermal Cycler using QuantaBio qScript cDNA Supermix The Illumina TruSeq Stranded mRNA Library Preparation Kit was used to prepare sequencing libraries of high-quality RNA samples (RIN > 7)