Whole Genome Sequencing of three Canadian strains of Escherichia coli from biosolids samples
Genomic DNA was extracted from an overnight culture in Tryptic Soy Broth using NanoBind CBB Kit (PacBio, US), followed by treatment with Short Read Eliminator XS (PacBio, US) according to the manufacturers instructions. Nanopore sequencing was performed by MinION library preparation using a native barcoding kit (SQK-NBD112.24) (Oxford Nanopore Technologies, UK) without shearing, and sequencing using an FLO-MIN107 (R10.4.1) flow cell on a MinION Mk1B device.