zea mays sample
The first strand of cDNA was synthesized in the M-MuLV reverse transcriptase system using fragmented mRNA as a template and random oligonucleotides as primers, followed by degradation of the RNA strand with RNaseH (RNAase H) and synthesis of the second strand of cDNA in the DNA polymerase I (DNA polymerase I) system with dNTPs (four deoxyribonucleotides). .