GSM7051237: MCF7 GREB1enhDel clone1 vehicle Rep1; Homo sapiens; OTHER
Cells were fixed with 1 .5 % formaldehide for 10 minutes. After lisis, nuclei were subjeted to in situ DpnII restriction and ligation. DNA was decrosslinked and extracted with phenol:chloroform. Regions of interest were captured from 3C libraries using fragmented and biotinilated tiled BACs. Captured regions' coordenates (hg19): chr2:11450601-11945472; chr21:16143548-17096678 Before Capture, 3C libraries were prepaired with NEBNext® Ultra™ II DNA Library Prep for Illumina