GSM8280003: in vitro keratinocytes, mCherry negative, anti-TNFalpha treatment, replicate 1; Mus musculus; RNA-Seq
mCherry negative keratinocytes cells were FAC- sorted on a BD ARIA II directly into TriZol LS and column purified with a Direct-zol™ RNA Miniprep kit (Zymo Research). Sequencing libraries from 1000ng of RNA per sample were prepared with the TrusSeq mRNA library kit and sequenced on a NovaSeq X 2x150bp at the Functional Genomics Center Zurich.