GSM7749262: CTR_dCas9_ChIP; Mus musculus; ChIP-Seq
Cells were fixed in culture medium at room temperature for 10 min with final 1% formaldehyde, and fixation was quenched by final 0.14M glycine at room temperature for 5 min. After cell lysis, sonication was performed using Fisher Scientific Sonic Dismembrator. About 1ng DNA was used for library construction using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB).