Juvenile male liver 1
Total RNA was isolated from each tissue using Trizol (Invitrogen, USA) and RNeasy Mini KIT (Qiagen, USA), genomic DNA residue was removed with TURBO DNAse treatment (Invitrogen, USA) and Ribo-Zero rRNA Removal Kit (Epicentre, USA) was utilized for rRNA depletion. Library preparation and RNA-seq were performed at BGI Genomics, China. NEBNext Ultra II Directional RNA Library Kit for Illumina (New England Biolabs, USA) was used for the construction of 16 libraries