GSM7666275: trxKD, trained, me1, rep3; Drosophila melanogaster; ChIP-Seq
Fly heads were obtained from samples of 50-60 flies through flash freezing with liquid nitrogen, followed by vortexing and separation through a series of a sieves. Fly heads were then suspended in homogenization buffer and nuclei released into solution through chemical and physical agitation of the cell membrane. Anti-GFP bound beads were then used to isolate GFP-positive nuclei from the whole head homogenate to represent the mushroom body-specific fraction (MB). Chromatin was isolated using the True ChIP-seq Kit (Diagenode: C01010132), according to manufacturer instructions. DNA was isolated using the MicroChIP DiaPure columns (Diagenode: C03040001). ChIP libraries were generated using the MicroPlex Library Preparation Kit (Diagenode: C05010001).