16S amplicon sequencing of lake Cadagno
Lake water was filtered on 0.2 m polycabonate filters. DNA was extracted with a hot phenol/chloroform method. The sequencing library was constructed with primers Bact341F: CCTACGGGNGGCWGCAG & Bact785R:GACTACHVGGGTATCTAATCC. Sequencing was done for 2x300 bp paired end reads.