GSM6825631: Arabidopsis, control of FRGA sly1 dP; Arabidopsis thaliana; ChIP-Seq
Tissue were fixed with 1% formaldehyde for 20 minutes. After cell lysis, chromatin was sonicated using Bioruptor (High power, 1 cycle = on-30 sec / off-30 sec). ChIP-seq libraries were prepared using the protocol in our lab.100-200 mg of ground tissue was used and 400 μL of chromatin and incubated at 4 ‎°C with rotation for 2 hr.