ITS2 (gITS7 and ITS4/ITS4arc16S) DNA meta-barcoding of root-associated eukaryotes under widespread trees across Europe
All PCR products were size checked by running 2 l of DNA on a 1% agarose gel for 15 to 20 min. The duplicate PCR amplicons were then pooled and cleaned using Sera-Mag bead purification (Cytiva Life Sciences)