GSM6383404: RV_d9.5_R3 [176164]; Homo sapiens; RNA-Seq
RNA was isolated using an in-house RNA bead isolation kit semi-automated using KingFisher devices (KingFisher Duo Prime). Using the QuantSeq 30 mRNA-Seq Library Prep Kit FWD (Lexogen GmbH, #015), the bulk RNA-seq library was generated according to the manufacturer's instructions. After the preparation of the library, samples were checked for an adequate size distribution with a fragment analyzer.Then the RNA-seq library was submitted to the Vienna Biocenter Core Facilities (VBCF) Next-Generation-Sequencing (NGS) facility for sequencing.