RNA-seq: PGCLC BPS 1uM rep 3
Total RNA was extracted from cells using Trizol and contaminating genomic DNA was removed by RQ1 DNase (M6101) treatment according to the manufacturers instructions (Promega Corporation, WI USA). Strand-specific RNA-seq libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina sequencing (E7760S) according to the manufacturers protocol (New England Biolabs, MA USA).