GSM8393265: GFP High 1; Mus musculus; RNA-Seq
Messenger RNA was purified from oocyte total RNA using poly-dT magnetic beads, and mRNA was fragmented. First strand cDNA synthesis was carried out using random hexamer priming, followed by second strand cDNA synthesis. Fragment ends were repaired, A-tailed, and ligated with sequencing adapters, followed by size selection, amplification via PCR, and purification. Final libraries were quantified using Qubit, and run on an Agilent Bioanalyzer system to check for proper size distribution. Post QC, libraries were pooled and sequenced on an Illumina sequencing platform. Clustering of the index-coded samples was performed as per the manufacturer's instructions, and libraries were subsequently sequenced to generate paired-end reads