GSM7412527: U2OS, Screen, Rep2 [ISG]; Homo sapiens; OTHER
Genomic DNA was extracted by phenol:chloroform:isoamyl alcohol and purified by ethanol precipitation. Three micrograms of total genomic DNA were used per PCR reaction with barcoded primers to produce a CRISPR amplicon library for each sample. Amplicon library samples were purified with AmpureXP beads and tested for purity by Agilent Bioanalyzer and qPCR with sequencing specific primers. Samples were quantified by Qubit, pooled, then sequenced on an Illumina NextSeq 500.