16S rRNA amplicon sequence: AMR plasmid transfer in natural bacterial community
Total bacterial genomic DNA extraction was performed using a E.Z.N.A.Soil DNA Kit (Omega, USA), following the manufacturers instructions. The concentrations of extracted gDNA were measured by a Qubit 2.0(life, USA) to ensure that adequate amounts of high-quality genomic DNA had been extracted. 16S rRNA gene libraries were constructed by amplified the bacterial 16S rRNA gene V3-V26 hypervariable region and the purified amplicons from each reaction mixture were pooled in equimolar ratios based on their concentration. Sequencing was performed using the Illumina MiSeq system (Illumina MiSeq, USA), according to the manufacturers instructions.