GSM7295199: LSM14B_WT_MI_Rep3; Mus musculus; RNA-Seq
We carried out the amplification by the Smart Seq2 method. An Oligo dT primer wa s introduced to the reverse transcription reaction for first strand cDNA synthesis, followed by PCR amplification to enrich the cDNA and magbeads purification step to clean up the production. purification step to clean up the production. Then the cDNA production was checked by Qubit ® 3.0 Flurometer and Agilent 2100 Bioanalyzer to ensure the expected production with length around 1~2kbp. Then the cDNA was sheared randomly by ultrasonic waves for Illumina library preparation protocol including DNA fragmentation, end repair, 3' ends A tailing, adapter ligation, PCR amplification and library validation.