GSM6938711: RAW264.7 cells- TBP- biol rep 2 m6A-IP; Mus musculus; RIP-Seq
Total RNA was extracted using TRIzol Reagent and MeRIP-seq samples were immunoprecipitated by m6A-specific antibody. The IP RNA was reverse-transcribed to cDNA, which was next used to synthesize U-labeled second-stranded DNAs. An A-base was added to the blunt ends of each strand, preparing for ligation to the indexed adapters. Dual-index adapters were ligated to the fragments, and size selection was performed. After the heat-labile UDG enzyme treatment of the U-labeled second-stranded DNAs, the ligated products were amplified with PCR.