GSM6732509: Patient 22 CD8+ [Pt22_CD8]; Homo sapiens; ChIP-Seq
5µg of chromatin was extracted from CD4+ and CD8+ sorted TIL and subjected to immunoprecipitation using 4µl of a H3 pan-acetyl antibody (Active Motif, cat #23139), per Active Motif's standard protocols. Pooled, unprecipitated genomic DNA was used as an input control. These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample.