GSM8311091: KEC, Asy ATAC, rep4; Mus musculus; ATAC-seq
Tagmented DNA was extracted with the MinElute PCR Purification Kit (Qiagen) Libraries were prepared using the NEBNext Ultra II Q5 2× Master Mix (NEB) and a unique combination of i5 and i7 adapters. The appropriate number of amplification cycles was calculated analyzing an aliquote of each library by real-time PCR using the NEBNext Library Quant Kit (NEB).