GSM6595160: G401 F2 TAZ BiolRep 3; Homo sapiens; RNA-Seq
RNA was isolated using RNeasy Mini, according to manufacturer's instructions (Qiagen). After RiboGreen quantification and quality control by Agilent BioAnalyzer, 149-500ng of total RNA underwent Poly(A) selection TruSeq library preparation according to instructions provided by Illumina (TruSeq Stranded mRNA LT Kit, catalog # RS-122-2102), with 8 cycles of PCR Samples were barcoded and run on a HiSeq 4000 in a 50bp/50bp paired end run, using the HiSeq 3000/4000 SBS Kit (Illumina)