GSM6938386: StRG47-Prog-hCD45-1 (TCR-seq); Mus musculus; RNA-Seq
Single cell suspensions of StRG colorectal tumors were sorted for CD45+ and collected into tubes containing PBS with 0.04% BSA. The cell suspensions were loaded on a Chromium Connect Single Cell Liquid Handler (10X Genomics) and RNA-seq and V(D)J libraries were prepared using Chromium Next GEM Automated Single Cell 5' Kit, v2 (10X Genomics)