uncultured control
Chromosomal DNA was extracted from the culture pellets using Promega Wizard Chromosomal DNA extraction kit (Promega) and subjected to PCR amplification using a pair of primers (QIAseq 16S/ITS Panels, Qiagen) designed to target the V2-V3 variable region of the bacterial 16S ribosomal RNA gene. The purified 16S rRNA amplicon was subsequently sequenced on the Illumina MiSeq platform