16S V4 sequence of bacteria
Isolates were pelleted, placed into 96-well plates, and DNA extracted using the MagAttract Microbial DNA kit and sequenced at the University of Michigan Center for Microbial Systems. They generated amplicons of the V4 region of the 16S rRNA gene using 515f/806r primers. DNA amplicons were sequenced on a 2 x 250 Illumina MiSeq v2 run.