GSM8185789: Bone marrow, sorted LSK, Vav1-iCre Control; Mus musculus; RNA-Seq
Bone marrow cells from 5 mice of each genotype were pooled, and Kit+ cells were purified using Cd117 microbead kit on AutoMACS separator (Miltenyi). Kit+ cells were flow sorted on MoFlo sorter (Beckman Coulter), to obtain LSK (Lin-Kit+Sca1+) cells. Sorted cells were resuspended at 1000/μL density in 1x PBS with 0.04% BSA. Cells were subjected to droplet bead capture, followed by cell lysis, reverse transcription, and amplification according to TotalSeqA protocol from 10X Genomics (Pleasanton, CA). TotalSeqA protocol from 10X Genomics (Pleasanton, CA).