GSM7873815: PXGL, H3K4me3, CRISPRi XIST Dox, Rep2; Homo sapiens; OTHER
Digested chromatin was treated with proteinase K and  eluted using phenol chloroform extraction as described in Skene et al.,2017. High-throughput sequencing libraries were constructed using the TruSeq ChIP Library Preparation Kit according to the manufacturer's recommendation. Paired-end sequencing was done on the Illumina NovaSeq 6000  with a read length of 100 bp.