GSM8281033: WT Treg_5 (omniATAC-seq) (Foxp3KO control); Mus musculus; ATAC-seq
ATAC-seq: Cells were lysed with cold lysis buffer (10 mM Tris-HCl (pH7.4), 10 mM NaCl, 3 mM MgCl2 and 0.1% IGEPAL CA-630). omniATAC-seq: Cells were lysed with cold lysis buffer (10mM Tris-HCl (pH7.4), 10mM NaCl, 3mM MgCl2, 0.1% NP-40, 0.1% Tween20 and 0.01% Digitonin) for 3 minutes on ice. ATAC-seq: DNA were labeled with Tagment DNA TDE1 Enzyme (Illumina) and amplified by PCR with 9-11 cycles using barcoded primers omniATAC-seq: DNA were labeled with Tn5 transposase (Diagenode) and amplified by PCR with 7 cycles using barcoded primers