GSM6387573: Brain microglia: Young (18C); Mus musculus; RNA-Seq
RNA was extracted from samples of FACS-sorted microglia with PureLink™ RNA Micro Kit (#12183016, Invitrogen) following manufacturer indications with minor modifications. RNA was precipitated with 70% ethanol overnight at -20°C. To avoid genomic DNA contamination a DNAse step was performed. RNA quantity and purity were assessed with the High Sensitivity RNA ScreenTape® (Agilent 2200 TapeStation system). Library Preparation - custom SMARTseq2 (NEBnext) (low input/non-stranded),