Genes of P. cantorii
A healthy 2-year-old P. cantorii reared in the breeding center of the Pearl River Fisheries Research Institute, Guangzhou, Guangdong, China, was used for genome sequencing and assembly. The turtle was immediately dissected after treatment with anesthetic tricaine methanesulfonate (MS-222, Sigma). Fresh muscle tissue was used for DNA extraction using a DNeasy Blood & Tissue Kit (Qiagen, Valencia, CA, USA) according to the manufacturers instructions. The DNA quantity and quality were measured using Qubit 3.0 and 1% agarose gel electrophoresis, respectively. High-quality DNA was used for library preparation, sequencing and Hi-C library construction. Library sequencing was performed using Illumina HiSeq4000 (Illumina, San Diego, CA, USA) and the PacBio platform.