amplicon seq 16S rRNA/ITS field 1
Amplicon sequencing analysis by targeting the V35/ITS2 region of the 16S rRNA gene for prokaryotes/fungi. A two-step amplification protocol was implemented, to construct amplicon libraries. A unique 12-bp index was added to the forward primer that served as a barcode to identify sequencing products in multiplexed libraries. 2x250 bp pair-end sequencing via the HiSeq2500 platform (Illumina, San Diego, USA) was performed by NOVOGENE UK.