Whole Genome Sequencing of three Canadian strains of Escherichia coli from biosolids samples
Genomic DNA was extracted from an overnight culture in Tryptic Soy Broth using NanoBind CBB Kit (PacBio, US), followed by treatment with Short Read Eliminator XS (PacBio, US) according to the manufacturers instructions. Illumina sequencing was conducted by library preparation using Illumina DNA Prep Kit (Illumina, US).