RNA-seq of Homo sapiens - Plasmodium ovale: B1
RNA was extracted from PAXgene tubes using the MagMax Blood RNA kit and used to prepare stranded libraries after rRNA and globin depletion using the NEBNext Globin and rRNA Depletion Kit (NEB) and polyA selection using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB). cDNA libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep for Illumina kit (NEB) and sequenced to generate paired end reads of 75 bp.