Mouse Terminal ileum metagenome, 7 days post Syngeneic BMT, replicate B
DNA was extracted from gut contents using the Qiagen MagAttract PowerMicrobiome kit (Cat: 27500-4-EP) and quantified with the Quant-iT PicoGreen dsDNA Assay kit (cat#: P7589). A spike-in control consisting of an even mix of DNA from three organisms (ATCC MSA-1014) was added to constitute 0.05% of the DNA. Shotgun metagenomic sequencing libraries were prepared by the University of Michigan Advanced Genomics Core using the NEBNext Ultra II FS DNA Library Prep Kit (NEB, E7805) and sequenced using an Illumina NovaSeq instrument (300 cycles, producing 150bp paired-end reads).