GSM6938363: VCaP_0nM_DHT_3; Homo sapiens; RNA-Seq
RNA from cells was isolated using Trizol (Invitrogen, Thermo Fisher Scientific), treated with RNase-free DNase set (Qiagen), and purified by Monarch RNA Cleanup Kit (New England Biolabs) according to manufacturers' protocols. The purified RNA was quantified by Qubit 4 Fluorometer (Invitrogen, Thermo Fisher Scientific) and Qubit RNA Broad Range Assay Kit (Invitrogen, Thermo Fisher Scientific), and its purity assessed by the 260nm/280nm ratio. RNA integrity was checked using 5300 Fragment Analyzer System (Agilent Technologies). Library preparation was performed using standard polyA enrichment and sequenced with a Novaseq 6000 (Illumina) in Novogene for 150 bp paired­end reads.