6 days C1
Total RNA was isolated from cells using TRIzol reagent (Invitrogen, Waltham, Massachu-setts, USA) according to the manufacturers protocol. The quality of the extracted total RNA was measured using Bioanalyzer 2100 (Agilent, Santa Clara, CA, USA). Total RNA with an RNA Integrity Number (RIN) value greater than or equal to 7 was used for library prepara-tion. RNA sequencing libraries were prepared using the TruSeq Stranded mRNA LT Sam-ple Prep Kit according to the manufacturers instructions (Illumina, San Diego, CA, USA). A total of nine libraries with their respective indices were paired-end sequenced using the HiSeq X system (Illumina).