GSM7017777: Wild-type replicate1; Saccharomyces cerevisiae; OTHER
Micro-C XL was performed following standard protocol from Hsieh et al., 2016. Briefly, exponential yeast cells were dual crosslinked with 3% formaldehyde and EGS and digested with appropriate amount of MNase to get >95% mononucleosomes. The chromatin pellet obtained was dephosphorylated, end-repaired and biotinylated through ligation, followed by isolation of dinucleosomal DNA from an agarose gel. The isolated DNA was processed for library preparation according to Illumina NEBNext protocol and paired-end sequencing was performed on an Illumina Nextseq 500 and Nextseq 2000 in the UVA Genome Analysis and Technology Core. Illumina sequencing protocol