GSM8230218: Brainstem_Pcoat+MB+_17mg per kg_Replicate3; Macaca mulatta; RNA-Seq
Brain, heart, kidney and liver tissues were collected immediately post-mortem in multiple repeats of 1 mm2 squares and preserved in RNAlater (Sigma-Aldrich). Total RNA was isolated by homogenizing in TRIzol (Thermo Fisher Scientific) and performing double extraction with guanidinium thiocyanate-phenol-chloroform followed by a Qiagen RNeasy Micro clean-up procedure. 500 ng of total RNA was subjected to Ribo-Zero (Illumina) ribosomal RNA depletion, and used for construction of sequencing libraries. Lexogen SENSE Total RNA-seq libraries were prepared according to the manufacturer's protocol.