Copepodid replicate 1
Approximately 30 mature egg sacs were picked from gravid females and hatched in a petri dish. Eggs were monitored for hatching and metamorphosis. Copepodid larvae were picked within 7-9 days post-hatching. The pool of larvae was then used for total RNA isolation, followed by DNase treatment and library preparation using Illumina TruSeq stranded mRNA kits.