Genomic sequencing of SARS-CoV-2: Clinical, USA:North Carolina
Viral sequencing was performed following a tiling amplicon strategy using the V3.1 primer scheme. Sequencing was performed using a PromethIon by Oxford Nanopore Technologies. Libraries were prepared using High-Capacity cDNA Reverse Transcription Kit by ThermoFisher, Q5® High-Fidelity DNA Polymerase pack by NEB, EB Buffer by Omega Biotek, NEBNext® Ultra™ II End Repair/dA-Tailing Module ,NEBNext® Ultra™ II Ligation Module and NEBNext® Quick Ligation Module by NEB, Sequencing auxiliary vials by ONT and Adapter Mix II Expansion by ONT.