RNA-Seq of Ca. Aquarickettsia rohweri from Acropora cervicornis tissue
Residual DNA contamination was removed from RNA isolates using the RQ1 RNase-Free DNase (Promega). Ribosomal RNA was removed using equal parts plant leaf, human/mouse and bacteria Ribo-Zero kits (Illumina). RNA quality and concentration were verified by BioAnalyzer (Agilent Technologies, Santa Clara, CA) and quantitative PCR, respectively. cDNA library prep and sequencing was performed at Oregon State Universitys Center for Quantitative Life Sciences (CQLS) Core Laboratories with the HiSeq 3000 platform.