GSM8394227: IP_OX_REP1; Mycobacterium tuberculosis; ChIP-Seq
Bacteria were fixed fixed with 1% formaldehyde for 10 minutes at 37°C. Glycine was added to quench the reaction. Bacteria were resuspended in Immunoprecipitation buffer and sonicated. ChIP-seq samples were prepared using NEBNext Ultra II Library Prep kit for Illumina. IP, MOCK and INPUT samples were PCR amplified according to the amount of starting material. Libraries' quality and size was checked on Bioanalyzer 2100 with Agilent DNA High Sensitivity Chips.