GSM7845165: Brain, Treatment, 13; Danio rerio; RNA-Seq
Brain samples were harvested from euthanised fish as per establishment protocol and flash frozen in liquid nitrogen before storage at -80 °C. In this study, the differential expression analysis was performed on a total sample size of n=40 (20 fish from the control diet group and the other 20 fish from the 60% GA experimental group). Samples were first homogenised then kept on ice before nucleic acid extraction. RNA extraction was performed using Quick-RNA™ Miniprep Plus Kit with Zymo-Spin™ IIICG Columns (Capped) & Spin-Away™ Filters (Zymo Research, product code: R1057, Cambridge Bioscience, UK) according to manufacturer's recommendations Extracted RNA samples were sent to Novogene for mRNA library preparation (poly A enrichment) then  for Illumina Sequencing (6G of raw data per sample)