Salivary gland transcriptome of Aphis gossypii
The sequencing libraries were generated using the KAPA RNA Hyper Prep Kit (Illumina Platforms, Roche Sequencing Solutions, Pleaston, CA) according to the manufacturer's instructions. Using magnetic oligo-dT beads, 1g of mRNA was purified from total RNA, which was then fragmented to the appropriate size using divalent cation (Mg2+) was accomplished by incubating it at 85 C for 5 minutes with Fragment, Prime, and Elute Buffer (1X). First strand cDNA synthesis was performed using reverse transcriptase and random hexamers, and second strand cDNA synthesis was done with RNase H and DNA Polymerase I. Adapters were ligated after A tailing of cDNA fragments, and then these products were purified by KAPA Pure beads. cDNA libraries were created by amplifying the ligated products by PCR amplification using KAPA Library Amplification Master Mix with sequencing primers. The quality of the cDNA libraries was assessed by Bioanalyzer (Agilent 2100) and the concentration was checked by Qubit. Finally, samples were sequenced on an Illumina Novaseq 6000 platform.