Nothofagus_antarctica_site_3.2_Invaded
Environmental DNA was extracted from rhizosphere soil using the moBio Powersoil Kit. Custom ITS1F and ITS2 PCR primers from IDT (containing barcodes and Illumina adapters) were used to amplify the ITS1 region of all fungi in the sample and sequencing was performed on an Illumina MiSeq instrument.