Whole exome of Homo sapiens: adult female blood
Whole exome sequencing and RNA-seq were performed at the LJI Sequence Core using Agilent whole exome capture and Illumina kits, respectively. FFPE biospecimens were distributed by the Moores Cancer Center to LJI for next generation sequencing along with matched reference DNA using peripheral blood specimens. Sequence reads from Exome-Seq of the tumor and normal samples were aligned to the reference genome GRCh38 using SpeedSeq Align (v0.1.0) (49). Exome variants were identified using SpeedSeq Somatic and variants were annotated using SNPeff (v4.3i) (50).