GSM8310921: Lesion M1-1, ROI 4555-2311; Homo sapiens; OTHER
FFPE brain samples were sectioned at 5 µm thickness. Sections were treated with Nanostring Whole Transcriptome Atlas probes according to the GeoMx® DSP Manual Slide Preparation User Manual (MAN-10150-03), and stained for IBA1. Whole transcriptome probes were collected from IBA1+ segments as according to the GeoMx® DSP Instrument User Manual (MAN-10152-03). Libraries were made from these probes according the GeoMx® DSP NGS Readout User Manual (MAN-10153-03). NanoString GeoMx® Digital Spatial Profiling libraries were loaded at 650 pM molarity on the Illumina NextSeq 2000 and sequenced with a P1 v3 100 bp reagent kit.