Long read sequencing of C. elegans wild isolates
Genomic DNA  was prepared using standard  phenol/chloroform extraction and ethanol precipitation. Samples were cleaned with DNA Clean  & Concentrator columns (Zymo Research #D4004), snap frozen with liquid nitrogen, and sent to the  Georgia Genomics and Bioinformatics Core (GGBC) at the University of Georgia. Quality was assessed by Qubit (Invitrogen) and NanoDrop (Thermo Scientific), molecular weight distribution was assessed by fragment analysis, and sizes >15kb were selected by BluePippin (Sage Science). Each sample was sequenced on a single SMRT Cell on the PacBio Sequel I platform.