GSM6732197: MDM-0 [N1876_N1900]; Homo sapiens; RNA-Seq
The  RNeasy  Plus  Mini  Kit (QIAGEN,  Hilden,  Germany)  was  used  to  isolate  RNA  and remove  genomic  DNA.  RNA  quality  was  assessed  with  the  2100  Bioanalyzer (Agilent Technologies,  Waldbronn,  Germany).  Samples  with  an  RNA  integrity  number (RIN) lower than  7  were  discarded. The PlM  from  3  healthy  adolescents  were  used  to  prepare  RNA  for transcriptome analysis. In parallel, MDM-0, and MDM-IL4 were utilized for polyA+ library preparation and for the mRNA-seq approach using a NextSeq550 short-read sequencer.