GSM6454897: embryo6-trunk, scRNAseq; Homo sapiens; RNA-Seq
Samples were incubated at 37°C for 10 minutes and were pipetted up and down thoroughly and gently every 3 minutes. To quench the dissociation, we added 300μl FBS into each tube and centrifuge cells at 300xg for 5 min at 4°C. Cells were then resuspended in 500μl cold PBS, filtered through a 35μm strainer. Single cells were subsequently washed twice with cold PBS, centrifuged at 300xg for 5 min at 4°C and resuspended in cold PBS. To generate sequencing libraries, a suspension of 10,000 single cells per sample was processed following the 10x Genomics protocol using Single Cell 3' Reagent Kits v3 (10x Genomics).