Whole Genome Bisulfite Sequencing of homo sapiens: jejunum CD103+ tissue resident memory T cell (TRM)
Purified genomic DNA was fragmented and bisulfite conversion was performed with EZ DNA Methylation-Gold Kit following the manufacturers instructions. WGBS library was prepared with Accel-NGS Methyl-Seq kit following the manufacturers protocol. A ~0.5% unmethylated lambda DNA spike-in was used as a bisulfite conversion rate control and for downstream bioinformatics. The final library was assessed with Agilent TapeStation.