RNA-seq of M13
The recovered phages were divided into three aliquots and stored in a 20 C refrigerator. One aliquot was 970 amplified for DNA extraction by M13 DNA Isolation Kit (Omega). The eluted DNA 971 was mixed with different sequencing barcodes and Primestar DNA polymerase (Takara) and further purified with GeneJET gel extraction kit (Thermo Fisher) to recover the 200- to 400-bp DNA fragment