GSM8141495: PDAC organoids from patient 50; Homo sapiens; RNA-Seq
Total RNA was extracted from the cultured PDO using ISOGEN reagent (NIPPON GENE, Tokyo, Japan) according to the manufacturer's recommended protocol. 500 ng of total RNA were ribosomal RNA-depleted using NEBNext rRNA Depletion Kit (New England Biolabs, Ipswich, MA, USA), and converted to Illumina sequencing library using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs).