GSM7932224: mHAR123, scRNAseq; Homo sapiens; RNA-Seq
Cells were dissociated into single cell suspension using Accutase (Gibco) and dead cells were removed using the ClioCell Dead Cell Removal kit (Amsbio) following the manufacturers' instructions. Viable cells were washed once in PBS and resuspended in 0.04% BSA in PBS for loading on the 10x Chromium chip. Cell capturing, and library preparation was carried as per kit instructions (Chromium Next GEM Single Cell 3' HT v3.1). In brief, 20,000 cells (10,000 cells per loading well) were targeted for capture per sample. After cDNA synthesis and library amplification, the resultant libraries were size selected, pooled and sequenced using 2 x 100 paired-end sequencing protocol on an Illumina NovaSeq 6000 instrument.