GSM8279940: WT5-MRNA-LFM18211; Mus musculus; RNA-Seq
Total RNA from tissues or cells was isolated using TRIzol (Invitrogen, Darmstadt, Germany), following manufacturer's instructions.1 ug of total RNA was used for the construction of sequencing libraries The poly(A) mRNA isolation was performed using Oligo(dT) beads. The mRNA fragmentation was performed using divalent cations and high temperature