GSM7296094: HK1-EBV pBabe HA IRES, Day 0; Homo sapiens; RNA-Seq
Cell were collected in ~350uL of TRIzol reagent. Total RNA was extracted from fresh frozen cell pellet samples using Qiagen RNeasy Plus Universal mini kit. RNA samples were quantified using Qubit 2.0 Fluorometer (Thermo Fisher Scientific) and RNA integrity was checked with 4200 TapeStation (Agilent Technologies). RNA purification, quantification and QA were performed by Genewiz. Trizol-purified total RNA samples were treated with TURBO DNase (Thermo Fisher Scientific) and subjected to rRNA depletion using QIAseq FastSelectTM−rRNA HMR kit (Qiagen). Strand-specific RNA sequencing libraries were prepared with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) using the dUTP incorporation method. Sequencing libraries were validated on the Agilent TapeStation (Agilent Technologies) and quantified on the Qubit 2.0 Fluorometer (Thermo Fisher Scientific) and by quantitative PCR (KAPA Biosystems). Sequencing libraries were multiplexed and clustered on one lane of a flowcell and run on an Illumina HiSeq instrument using a 2x150bp Paired-End (PE) protocol. Library construction, QC/QA, and sequencing were performed by Genewiz.