Single-cell analysis of human fetal bone marrow
Single-cell analysis of human fetal bone marrow
The obtained samples are quickly placed into EP tube with PBSbuffer for flushing the blood. Then, the samples were cleanedin cell preservation solution. Finally, the cleaned samples wereplaced into a sterile EP tube with 2 mL cell preservationsolution before being stored in a refrigerator at 4 ° C. The cells were sorted as single-cells using the Chromium 10X Genomics platform. Approximately 7,000 cells were loaded per channel onto a Chromium controller (10x Genomics) for generation of gel bead-in-emulsions using 10x Single Cell 3' v3. Sequencing libraries were prepared using Single Cell 3' Reagent Kits v3 (10x Genomics)