GSM6595569: p53LOF2_B; Danio rerio; RNA-Seq
After tissue grinding and homogenization in RNA lysis buffer, total RNA was isolated from harvested tumors using the RNAeasy Kit (Qiagen; Valencia, CA) according to the manufacturer's recommendation. After quantification with Nanodrop (Thermo Scientific; Rockford, IL), RNA was stored at -80ºC. Total RNA was used for RNA-Seq library preparation by following the KAPA Stranded RNA-Seq Kit