GSM6939850: SGm Rep.1; Mus musculus; ChIP-Seq
Flash frozen fSMO;GFAP-cre MB tissues from males and females were sent to Active Motif (Carlsbad, CA) for ChIP-Seq analysis, using a previously ChIP validated anti-YAP1 antibody (US Biological, Salem, MA; # Y1200-01D- RRID:AB_2927438). Illumina sequencing libraries were prepared from the ChIP and Input DNAs by the standard consecutive enzymatic steps of end-polishing, dA-addition, and adaptor ligation. Steps were performed on an automated system (Apollo 342, Wafergen Biosystems/Takara). After a final PCR amplification step, the resulting DNA libraries were quantified and sequenced on Illumina's NextSeq 500 instrument.