GSM6939407: Small intestine ILC3, pellet of meRIP, rep2; Mus musculus; RNA-Seq
RNA was harvested from the IL-1 plus IL-23-induced ILC3 in small intestine using miRNeasy Micro Kit (Qiagen). Then m6A immunoprecipitation (meRIP) was performed to obtain the unbound and pellet fractions. RNA of unbound and pellet fractions were used for the construction of sequencing libraries. cDNA libraries for RNA-seq were prepared using NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina® following manufacturer's protocols.