GSM7979535: ZSCAN4, with Dox, ChiP; Mus musculus; ChIP-Seq
Genomic  paired end DNA libraries were constructed for sequencing on the Illumina platform using the KAPA Library Hyper Preparation Kit (Kapa Biosystems, Woburn, MA). 60 ng of DNA was fragmented with the Covaris E220( Woburn,MA). Then libraries were prepared using a modified version of manufacturer's protocol. The DNA was purified between enzymatic reactions and the size selection of the library was performed with  SPRIselect beads (Beckman Coulter Genomics, Danvers, MA).  Samples were indexed during a final amplification using primers designed to amplify on an illumina hiseq 4000.