GSM7719608: CA1 hippcampus, treated, 24h, replicate 3; Mus musculus; RNA-Seq
Mice were deeply anesthetized following decapitation and the brain was rapidly removed. Hippocampal slices were prepared by slicing the brain into coronal slices (400 μm) on a Thermo scientific HM650V microtome in ice-cold Phosphate Buffer Solution  (140 mM NaCl, 10 mM phosphate buffer, and 3 mM KCl, pH 7.4 solution). CA1 hippocampus region was carefully microdissected under a microscope. Samples were subsequently mechanically homogenized for total RNA isolation. Total RNA was isolated using the miRNeasy micro kit (Qiagen) according to the manufacturer's protocol. The quality and integrity of total RNA was assessed using a Tapestation 2200 (Agilent Technologies). Purification of mRNA was performed using polyT oligo-attached magnetic beads. After purification, mRNA was used for library preparation using the TruSeq RNA Library Prep Kit v2 (Illumina) according to the manufacturer's protocol. Sequencing was carried out on a HiSeq1500 system (Illumina) using cycle paired-end sequencing.