Mut_s2_Sample1
TruSeq Stranded mRNA Sample Preparation. Ribodepletion using riboPOOLs kit (siTOOLs Biotech). Chemical fragmentation. First strand sytnthesis using random primers. dTTP replaced by dUTP during second strand synthesis. A base addition on extremities. Anchor ligation. PCR amplification that also allows indexing. Library quality check using Fragment analyzer and qPCR.