GSM7408937: single_cell BCR seq data of patient EOMG07; Homo sapiens; OTHER
Enrichment of CD45-positive cells was performed using CD45 (TIL) microbeads (Miltenyi Biotec., 130-118-780) according to the manufacturer's protocol. Cell number and viability were checked before and after the enrichment to ensure that no significant cell death has been caused by the process. CD45+ MACS-enriched single-cell suspensions were loaded onto the Chromium Single Cell Controller using the Chromium Next GEM Single Cell 5' Library & Gel Bead Kit v1.1 chemistry (10x Genomics). The derived barcoded complementary DNA (cDNA) was partly used in Chromium Single Cell V(D)J Enrichment Kits (10x Genomics) for further scBCR-seq and scTCR-seq. Sample processing and library preparation were performed according to the manufacturer's instructions using SPRIselect beads (Beckman Coulter).