16S Sequencing of anaerobic digester
For 16S rRNA gene PCR amplification, genomic DNA was added as template into a total reaction volume of 25 L. Dual-indexing strategy and a universal primer set 505F (5 - GTGCCAGCMGCCGCGGTAA-3)/806R (5-GGACTACHVGGGTWTCTAAT-3) targeting the V4 region of both bacterial and archaeal 16S rRNA gene was amplified