GSM6387303: Gastroluid_60h_CHIR replicate 1; Mus musculus; ChIP-Seq
Mouse embryonic stem cells grown in 2i LIF media were harvested and re-suspended in N2B27media containing FGF2 (100ng/ml). 300 cells were plated in 40ul volume per well of low-adherence round-bottom 96-well plate and incubated at 37 °C for 48h and subsequently stimulated with 150ul of 3uM CHIR in N2B27. Samples were collected at 48h and 60h as well as 0h untreated control. DNA fragment distribution was assessed via High Sensitivity D1000 ScreenTape on the TapeStaton (Agilent) and quantity assessed using the QuantiFluor dsDNA System (Promega) measured on the GloMax system (Promega).  DNA was normalised to 0.1 ng and converted into Illumina compatible libraries using the NEBNext Ultra II DNA Library Prep Kit (New England BioLabs) according to manufacturer's instructions.  Sequencing was performed on the HiSeq 4000 (Illumina) using single ended reads of at least 75 bp.