GSM7874741: mut.009; Mus musculus; RNA-Seq
Total RNA was isolated from three E17 kidneys for each genotype using an RNeasy Mini Kit (Qiagen 74104) with on the column DNAse I treatment. Ribosomal RNA was depleted from 1 μg of total RNA using Ribominus Eukaryote System v2 (Life Technologies). Construction of barcoded sequencing libraries was performed using the Ion Total RNA-seq v2 kits (Life Technologies) according to the manufacturer's instructions.