GSM6387635: miR-145 Lung; Mus musculus; RNA-Seq
Lungs from postnatal day 12 mice were collected and washed in cold PBS. Tissue was cut into pieces and digested in 2mg/ml Collagenase Type II (Worthington Biochemical, LS004174) for 45 minutes at 37°C, pipette mixing was carried out to dissociate cells every 10 minutes. Cells were filtered through 100µm and 70µm cell strainers (BD Falcon) and treated with red blood cell lysis buffer (Qiagen) for 5 minutes. Cells were resuspended in 250µl culture media (10% Fetal Bovine Serum, DMEM High Glucose with Glutamine, Penicillin Streptomycin). Cell viability (>85%) was measured using the Automated Cell Counter (Thermo Fisher, AMQAF1000). Chromium Single Cell 3' Reagent Kit v2 controller (10x Genomics)