Jinze 2011 sample sequence reads
DNA was extracted from 0.5 to 1 g (wet weight) of sediment and biomass-containing filters using a FastDNA spin kit for soil (MP Biomedical, OH, USA)according to the manufacturers instructions. DNA quality was assessed using a NanoDrop ND-1000spectrophotometer (NanoDrop Technology, USA). The extracted DNA was sheared to 500 bp using aCovaris Ultrasonicator (Covaris Inc., Woburn, MA, USA) following the manufacturers recommendation. The sheared DNA was then subjected to end pairing and to adaptor ligation with multiplexing and was purified using an Ovation SP ultralow DR multiplex system.