BaRD-seq of Human immunodeficiency virus 1:03-DMSO_Spleen
we ligated a L-shape adapter on the randomly fragmented cellular genome. A 21-nucleotide unique molecular identifier (UMI) was included in the adapter to label each proviral molecule. Multiple steps of semi-nested PCR enriched the fragments including the integration junction. The PCR product was enzymatically digested and self-circularized to bring the barcode and the integration site together. Then the short fragments containing UMI, barcode and the integration site were amplified for high-throughput sequencing.