GSM7663837: RNAseq wild type MEF replicate 3; Mus musculus; RNA-Seq
For iCLIP, cells were washed twiced with ice-cold PBS and crosslinked with UV254 at 150 mJ/cm^2/sec.  Cross-linked cells were then lysed in lysis buffer (20 mM Tris pH 7.4, 1% SDS, 5 mM EDTA).  For miCLIP, RNA was extracted with Trizol followed by two rounds of poly-A purification. iCLIP and miCLIP libraries were prepared as previously described (PMID: 27602518, PMID 30874375) iCLIP, miCLIP, RNAseq