GSM8308778: KO; Mus musculus; OTHER
CD4 SP T cells were isolated by negative selection using the EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit (# 19765, STEMCELL Technologies, Vancouver, Canada) according to the manufacturer's instructions, from single cell thymocyte suspensions prepared from B6.Btla WT and B6.Btla-/- mice (aged 8-12 weeks). TCR sequencing was conducted using the 10x Genomics V(D)J workflow. Briefly, single-cell TCR libraries were prepared using the Chromium Single Cell Mouse TCR Amplification Kit (Catalog #1000254). Libraries were pooled to achieve desired quantities for appropriate sequencing depths, as recommended by 10x Genomics, and were sequenced on an Illumina NovaSeq 6000 (v1.5) instrument.