GSM6938458: A549 cells, hypoxia, TGFβ treatment, Day0, biol rep 1; Homo sapiens; RNA-Seq
RNA was isolated using RNeasy Mini kit (Qiagen) . According to the manufacturer's protocol, amplified cDNA was then generated using the SMARTer Ultra Ultra-Low-input RNA kit, version 4 (Clontech Laboratory) followed by Nextera® XT DNA Library Preparation kit (Illumina) for sample barcoding and fragmentation.