GSM7299603: KOPN cells, sgRNA empty vector control, ATAC, biol rep1; Homo sapiens; ATAC-seq
Cells were lysed via centrifugation for 1 minute at 500xg. Nuclei were tagmented using Nextera (Illumina) Tagmentation DNA buffer and enzyme. libraries were prepped according to protocol by Buenrostro et al. Briefly, PCR was performed using barcoded primers that recongizne the adapter sequences inserted during the transpase reaction.