NextSeq 500 paired end sequencing; DRIP-seq files for: DDX17 is required for efficient DSB repair at DNA:RNA hybrid deficient loci
DRIP-seq files for: DDX17 is required for efficient DSB repair at DNA:RNA hybrid deficient loci
Cell trypsinisation and pelleting via centrifugation. either 300nM 4-hydroxytamoxifen or equivalent ethanol volume treatment for 4 hours.  Cytoplasmic lysi, nuclear pelletin following by SDS-proteinase K extraction at 55C for 1 hour. Finally ethanol precipitation and spooling of precipitated DNA. NEB NEBNext Ultra II DNA Library prep kit