GSM8232012: FB, cytoplasm, replicate1; Homo sapiens; RNA-Seq
Total RNAs from cultured cell line or cultured cells with different treatments were extracted with Trizol Reagent (Invitrogen) according to the manufacturer's protocol. To profile RNA subcellular distribution in FB neuron cells, total/cytoplasmic/nuclear RNAs were processed with the RiboMinus Transcriptome Isolation Kit (Human/Mouse Module, Invitrogen) to deplete ribosomal RNAs for sequencing (ribo- RNA-seq) The ribo- RNA-seq libraries from total/cytoplasmic/nuclear RNAs in  FB neuron cells  were prepared using Illumina TruSeq Stranded Total RNA LT Sample Prep Kit, followed by size selection using AmpureXP beads (Agencourt). Size-selected libraries were quantiﬁed with the Agilent Bioanalyzer 2100 and QuBit high-sensitivity dsDNA assay, and then subjected to deep sequencing with Illumina NovaSeq 6000 system at Sequanta Technologies. Raw read qualities were evaluated by FastQC (http://www.bioinformatics.babraham.ac.uk/projects/fastqc/).