GSM7666311: MDSC_DAB; Mus musculus; RNA-Seq
Single cell suspension of DMSO- or DAB-treated MDSC were washed twice in PBS and filtered through a 100μm cell strainer. Cell were then resuspended in PBS+0.04% BSA and loaded into the 10X Chromium Controller using the Chromium Next GEM Single Cell 5' Kit v2 (10x Genomics). Protocol number CG000331 RevB from 10X Genomics (User Guide, Chromium Next GEM Single Cell 5' Reagent Kits v2, Dual Index) was used.