GSM7845036: 7PPPn; Bos taurus; Bisulfite-Seq
DNA was isolated from tissue using using Sherlock AX kit (A&A Biotechnology, Poland). The RRBS libraries construction was performed using Ovation® RRBS Methyl-Seq System 1–16 (NuGEN Technologies, San Carlos, CA, United States), following the manufacturer's protocol. Sequencing was performed using an Illumina NextSeq550 system with a High Output kit v2.5 and 1 × 75 sequencing cycles. To control the quality of the reads obtained and increase RRBS library complexity at early sequencing cycles, 30% phiX genomic DNA (Illumina, San Diego, California) was added to the libraries.