WGS of NCI-PC155 organoid
DNA was extracted from organoids using the Qiagen AllPrep DNA kit. 1ug of DNA was sheared and assembled into whole genome sequencing libraries using the Illumina TruSeq DNA Library Preparation Kit. Samples were sequenced on the Illumina platform. Pass-filter FASTQ files were trimmed using Trimmomatic, aligned with BWA-MEM, duplicate-marked and quality-score recalibrated using GATK version 4.