RNA-Seq of Bovine EBL cells
EBL cells after Mycoplasmas bovis infection for 72h were collected for RNA-Seq profiling. Total RNAs were isolated using the Trizol reagent. The mRNAs were enriched using the Oligo(dT) beads and then were fragmented, reversely transcribed into cDNA. The cDNA Library quality was checked using a Bioanalyz-er2100 (Agilent). The cDNAs were purified with QiaQuick PCR extraction kit (Qiagen), end repaired, poly(A) added, and ligated to Illumina sequencing adapters. The ligation products were size selected by agarose gel electrophoresis, PCR amplified, and sequenced using Illumina HiSeq 2500 by Gene Denovo Biotechnology. The test sample is repeated for three times. Infected cell samples: C72-1-R1, C72-1-R1 and C72-3-R1, Comtrol cell samples: A72-1-R1, A72-2-R1 and A72-3-R1.