Nanopore reads of CRISPR-targeting RP62A challenged with Twillingate WT
DNA was extracted using ZymoBIOMICS and mechanically lysed; libraries were prepared with ONT's PCR-free Ligation Kit and NEBNext Companion Module, without fragmentation or size selection. Sequencing was done on Oxford Nanopore's MinION or GridION with R10.4.1 flow cells and Guppy for basecalling.