GSM7845329: Cortex_MHCII+F4/80+_rep3 [0200907641]; Mus musculus; RNA-Seq
Endocrine (CD45-) and macrophage (CD45+ F4/80+ 545 MHCII+ CX3CR1-GFP+) cell populations were FACs sorted into Buffer RLT Plus (Qiagen) and RNA was extracted using the RNeasy Plus Micro Kit (Qiagen) according to manufacturer's instruction. Reverse transcription and cDNA pre-amplification were performed using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech/Takara). cDNA was harvested and quantified with the Bioanalyzer DNA High-Sensitivity kit (Agilent Technologies). Libraries were prepared using the Nextera XT DNA Sample Preparation Kit and the Nextera Index Kit (Illumina) as per manufacturer instructions. Multiplexed libraries were pooled and paired-end 150-bp sequencing was performed on the Illumina HiSeq 4000 platform.