rav1 root, replicate 1, 14 dag
Total RNA was extracted using the RNASure Plant Mini Kit (Genetix brand, NP-84905) as described by the manufacturer. >2 ug of total RNA was used for the construction of sequencing libraries. 500ng of Total RNA was used to enrich the mRNA using NEBNext Poly (A) mRNA magnetic isolation module (Catalog: E7490, New England Biolabs) by following the manufacturers protocol. The enriched mRNAs were further taken for the library preparation using the NEBNext Ultra II RNA Library Prep Kit for Illumina (Catalog: E7775S, New England Biolabs).