GSM7666932: EFZ836046AR; Homo sapiens; OTHER
Takara Bio SMARTer Human TCR profiling kit Libraries for TCR-seq were prepared from 100 ng of total RNA using the SMARTer Human TCR a/b Profiling Kit v.2 (Takara Bio). Eighteen cycles were used for each of the two seminested PCR ampli- fication steps. Sequencing was performed on an Illumina NovaSeq by multiplexed paired-read run with 2 × 251 cycles. Qiagen RNA & DNA all-prep with clear up