BOKHARY2023:WHOLESTILLAGEAD:10HRT
Library preparation was performed using a 16S rDNA library preparation kit (Azenta Life Science, US). Samples were multiplexed, barcoded, and analyzed in a single run. The pooled DNA libraries were loaded on a MiSeq instrument (Illumina, San Diego, CA, US) using a 2x250 paired-end configuration. Image analysis and base calling were performed by Illumina Control Software on the Illumina instrument.