GSM6560983: m6A-seq-L-EV-bio rep 1; Homo sapiens; OTHER
extracted total RNAs with high quality were sent for rRNA depletion with human TransNGS rRNA Depletion Kit (TransGen Biotech, Beijing, China) and RNA purification using MagicPure RNA beads (TransGen Biotech). RNAs were then segmented into 60~200 nt fragmentations with 10% of total RNA removed as input control. For immunoprecipitation, RNAs were incubated with Dynabeads Protein A (Thermo Fisher Scientific) and m6A antibody at 4 °C for 2 hours. As to MeRIP-Seq, immunoprecipitated and purified RNA fragments as well as input RNAs were then sent for library construction using the VAHTS Total RNA-Seq (H/M/R) Library Prep Kit for Illumina (Vazyme, Nanjing, China)