GSM8140478: RNA-seq ∆rlp2 (∆NCU02898) strain: N6707; Neurospora crassa; RNA-Seq
Samples were cleared by centrifugation, and the aqueous (top) phase was mixed with 350uL of chloroform. After centrifugation, the aqueous phase was divided into two tubes containing 650uL cold 100% EtOH.  Samples were precipitated at -20oC, pelleted by centrifugation, washed twice with 1mL 70% EtOH, and resuspended in DEPC-treated dH2O. RNA was treated with DNaseI, amplification grade (Thermo Fisher Scientific) and cleaned (Agencourt® RNAclean XP® beads, Beckman Coulter). RNA-seq libraries were prepared using the KAPA Stranded mRNA-seq kit (KAPA Biosystems).