GSM7666615: BMDM cells, mannose sugar positive control, 24 h, biol rep 2; Mus musculus; RNA-Seq
Total RNA that was extracted by a Qiagen RNeasy kit (Qiagen, cat#74104) with RNase-free DNase Set (Qiagen, cat#79254) for NGS library preparation and sequencing Libraries were prepared using the Illumina TruSeq Stranded mRNA Library Preparation Kit with IDT for Illumina TruSeq Unique Dual Index adapters following manufacturer's recommendations. Completed libraries were QC'd and quantified using a combination of Qubit dsDNA HS and Agilent 4200 TapeStation HS DNA1000 assays