FACS-sorted NHBE CD66c+ "other" cells, pneumacult treatment group, replicate 1 of 3
NHBE cells were grown for 3 weeks at air-liquid interface and sorted into cell populations based on flow cytometry markers using FACS: secretory, ciliated, basal, triple negative, and CD66c+ "other" cells. Undifferentiated NHBE's that are not grown at air-liquid interface were also included. RNA extracted using RNeasy Plus Micro Kit, and cDNA libraries were prepared with the Takara SMRT-Seq mRNA LP kit.