GSM8230125: MEF_32_Prrx1_KO_rep1; Mus musculus; RNA-Seq
Total RNA isolation of MEFs was performed using RNAiso Plus (TAKARA) according to manufacturer's instructions. Total RNA was converted to cDNA using HiScript II Q RT SuperMix (Vazyme). RNA libraries for RNA-seq were prepared using NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® following manufacturer's protocols.