Whole genome sequencing of caribou muscle tissue
Samples were extracted using a Qiagen DNAeasy tissue extraction kit following the manufacturer's instructions (Qiagen). Samples were run on a Qubit fluorometer (Thermo Fisher Scientific) using the High Sensitivity Assay Kit and normalized to 20 ng/l at a final volume of 50 l. The DNA was shipped to The Centre for Applied Genomics (TCAG) at the Hospital for Sick Children (Toronto, Ontario) for library preparation and sequencing. The samples were sequenced two per lane on an Illumina HiSeq X.