GSM7665667: cKit+Mac1+ cells, Enl-T1, H3K27me3, CUT&Tag; Mus musculus; OTHER
Lysates were clarified from nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq sample libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit (NEB, #E7645L) following the manufacturer's instructions.  After adapter ligation, DNA was PCR amplified with Illumina primers for indicated cycles and the DNA size distribution was quantified on an Agilent Bioanalyzer DNA chip ( Agilent, #5067-1504). Then samples were sequenced using Illumina NextSeq2000. CUT&Tag sample libraries were prepared using the CUTANA™ CUT&Tag Kit (EpiCypher, #14-1102) following the manufacturer's instructions.  After getting the library, the DNA size distribution was quantified on an Agilent Bioanalyzer DNA chip ( Agilent, #5067-1504). Then samples were sequenced using Illumina NextSeq2000.