Laccase producer
For genome extraction, 100 L aliquots of C. unicolor 87613 fermentation culture were spread on cellophane-overlaid potato dextrose agar medium (PDA), and incubated at 30  for 5 d. The integrative genome was extracted from cultures of C. unicolor 87613 using cetyltrimethylammonium bromide (CTAB) method. Briefly, less than 1 g cultures were grinded in liquid nitrogen and dissolved into 1 mL preheated buffer containing 2% CTAB to destroy the structure of cell membrane and release the nucleic acids. The suspension was incubated in 65  water bath, followed by protein removal with phenol/ chloroform/ isoamyl alcohol (25:24:1) and chloroform/ isoamyl alcohol (24:1), and genome precipitation with isopropanol. For next generation sequence, the precipitated genome was dissolved in water and used for library construction with the NEBNextUltra DNA Library Prep Kit (NEB, USA). Library preparation and high-throughput sequencing were performed at Novogene (Beijing, China), using Illumina NovaSeq.