eccDNA_WT_stress_Flg_rep2
Surface-sterilized Col-0 seeds were resuspended in 0,1% agarose and transferred to solid  MS medium supplemented with sterile filtered 4.6 ug/mL -amanitin (Sigma-Aldrich, CAS 23109-05-9) and 9 ug/mL zebularine (Sigma-Aldrich; CAS 3690-10-6). For flagellin treatment, 2-weeks old plants grown on  MS media supplemented with -amanitin and zebularine were transferred to the equal ZA medium but also supplemented with 0.5 ug/mL flagellin (Flagellin from Salmonella typhimurium, Sigma-Aldrich, SRP8029-10UG). For ABA treatment, 2-weeks old plants grown on  MS media supplemented with -amanitin and zebularine were transferred to the equal ZA medium but also supplemented with abscisic acid at final concentrations 100 uM. For heat-stress treatment 2-weeks old plants grown on  MS media supplemented with -amanitin and zebularine were subjected to elevated temperature (4C for 24 h followed by 37C for 24 h, HS). For mild heat stress treatment 2 weeks-old Col-0 ddm1 plants were subjected to 30C for 24 h.  EccDNA enrichment was performed using protocol Lanciano et al., 2017.