Union Glacier fungal 16S ITS2 region metabarcoding from Edson Hills replicate 1
Total DNA extraction was performed with the FastDNA Soil Kit (MP Biomedicals). Three different molecular markers were amplified and sequenced using the Illumina NovaSeq system. For bacterial analysis we selected the V3-V4 region of 16S rRNA gene (primers 341F 5'-CCTAYGGGRBGCASCAG-3' and 806R 5'-GGACTACNNGGGTATCTAAT-3'). For archaeal analysis we utilized the V4-V5 region of 16S rRNA gene (primers Arch519F 5'-CAGCCGCCGCGGTAA-3' and Arch915R 5'-GTGCTCCCCCGCCAATTCCT-3'). For fungal analysis we used the region ITS2 (primers ITS3F 5'-GCATCGATGAAGAACGCAGC-3' and ITS4R 5'-TCCTCCGCTTATTGATATGC-3').