JHSC-0287_RNA
Samples have been submitted for RNAseq using the Illumina RNA Access hybrid capture-based protocol. All samples were treated with DNAse I prior to library generation according to manufacturer's protocol. Sequencing was performed on an Illumina NovaSeq instrument with 100M PE configuration. 40M reads were used for each sample. Fastq files were quality trimmed using trimmomatic and aligned to the GRCh38 transcriptome using salmon v1.6.0.