GSM6820635: regeneration_5 time-point; Schmidtea mediterranea; OTHER
Regenerating planarian fragments of different time points were killed by 5% NAC in PBS for 1 minute, embedded using pre-cooled OCT, and placed in a -80 °C refrigerator for storage. The tissues were placed into -20 °C freezing microtome for acclimation 30min before sectioning. Agilent 2100 bioanalyzer was used to assess the RNA quality of cryosections. Continuous cryosectioned 10 µm to the largest section cutting plane and collected for spatial transcriptomics sequencing (10x Genomics, Pleasanton, CA, USA). Libraries were constructed according to the Visium Spatial Gene Expression User Guide (10X Genomics, CG000239).