GSM6387337: SPF_Kpn_3; Mus musculus; RNA-Seq
Aorta tissues were collected, and RNA was extracted using the RNeasy (Qiagen) kit with DNase. RNA quality was assessed on a NanoPhotometer spectrophotometer and Agilent 2100 bioanalyzer. Total RNA underwent mRNA enrichment with the NEBNext Poly (A) mRNA Magnetic Isolation Module, followed by library preparation with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina. Libraries were validated with the MultiNA Bioanalyzer, and barcoded samples were pooled to equimolar concentrations before sequencing. Cluster generation was performed with HiSeq 2500. Paired-end sequencing was performed at Novogene Bioinformatics Technology Co., Ltd. Cleaned RNA sequencing reads were obtained through quality control and blasted with a mice genome in HISAT2 software Library preparation was performed with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina