GSM7665969: WT.M2.D.1; Mus musculus; RNA-Seq
RNA was isolated with Zymo Microkit (cat. #1050) 3' mRNA-Seq libraries were generated from 100 ng of DNAse I treated total RNA using the QuantSeq FWD Library Preparation kit (Lexogen, Vienna, Austria), according to manufacturer's directions. Overall library size was determined using the Agilent Tapestation and the DNA 5000 Screentape (Agilent, Santa Clara, CA). Libraries were quantitated using real-time PCR (Kapa Biosystems, Wilmington, MA). Libraries were pooled and Single read, 75 base pair Next Generation Sequencing was done on a P2 flow cell using the NextSeq 2000 (Illumina, San Diego, CA).