Full-length cDNA sequencing of ampliconic genes from human testis (A014a technical replicate 2)
Total RNA was extracted from testis sample using the RNeasy Mini Kit following the manufacturer recommended protocol (Qiagen, USA). Two cDNA technical replicates were generated with SMARTer PCR cDNA Synthesis Kit (Clontech, USA). These cDNAs were used as templates for a selective pulldown of polyadenylated ampliconic gene cDNAs using double hybridization of designed in-house biotinylated capture probes (a total of 10 probes, one for each of the nine ampliconic gene transcript families was used except for  VCY gene cDNAs captured using human- and chimp/bonobo-specific probes).