GSM8140355: shIfnar2; Mus musculus; OTHER
In brief, 0.1 million cells were collected for each sample. The following primary antibodies were used rabbit anti-HA antibody HA-Tag (CST, #3724s,1:500). Goat anti-Rabbit antibody (1:100; Vazyme) was used as the secondary antibody. pAG-Tn5 was purchased from Vazyme and used for each CUT&Tag reaction building a library according to the Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro protocol (Vazyme, #TD904, Nanjing, China). ChIP-seq libraries were prepared using the VAHTS Universal DNA Library Prep Kit (Vazyme).