GSM8231980: Whole mouse pyloric tumour; Mus musculus; RNA-Seq
The isolated tumour was incubated in a dissociation buffer containing collagenase I. Thereafter, the cells were mechanically dissociated by vigorous pipetting before treatment with TrypLE and DNase I to obtain a single cell suspension. Library construction was performed with the 10x Genomics Chromium Next GEM Single Cell 3' Reagent Kit (v3.1), following manufacturer's instructions.