GSM8308811: ATAC_BaF3-1; Mus musculus; ATAC-seq
Cell pellets were then resuspended in 50ul cold lysis buffer containing 10 mM Tris-HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl2, and 0.1% NP-40. Lysed nuclei were spun down immediately at 500g for 10min at 4°C, and cell pellets were resuspended and treated with a transposase reaction mixture containing 2.5ul Tn5 transposase at 37°C for 45 min. The fragmented DNA was purified using the MinElute Kit (QIAGEN). ATAC-seq assay was performed using the Nextera DNA library preparation kit (Illumina).