GSM6825461: Hela cells, shFBXO38 2; Homo sapiens; RNA-Seq
RNA was isolated with EZ-press RNA Purification Kit (B0004D, EZBioscience) and submitted to the Personalbio Laboratory for stranded library preparation. Sequencing libraries were generated by using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) and the quality was assessed on the Agilent Bioanalyzer 2100 system. After cluster generation, the library preparations were sequenced on an Illumina Novaseq platform and 150 bp paired-end reads were generated.