GSM7299601: 697 cells, sgRNA SETD2 clone BM_B, ATAC, biol rep2; Homo sapiens; ATAC-seq
Cells were lysed via centrifugation for 1 minute at 500xg. Nuclei were tagmented using Nextera (Illumina) Tagmentation DNA buffer and enzyme. libraries were prepped according to protocol by Buenrostro et al. Briefly, PCR was performed using barcoded primers that recongizne the adapter sequences inserted during the transpase reaction.