Whole Genome Sequencing of Porphyromonas gingivalis isolate EC324: Clonal Isolate
Extracted genomic DNA was converted to a library using the Nextera XT Library Prep Kit (Illumina, San Diego, USA) following the manufacturer's protocol with the following modifications: input DNA increased 2-fold, and PCR elongation time increased to 45 seconds. DNA quantification and library preparation were carried out on a Hamilton Microlab STAR automated liquid handling system (Hamilton Bonaduz AG, Switzerland). The library was sequenced on an lllumina NovaSeq 6000 (Illumina, San Diego, USA) using a 250 bp paired end protocol. Reads were adapter trimmed using Trimmomatic version 0.30 with a sliding window quality cutoff of Q15.