GSM6383613: 1_NFA; Mus musculus; RNA-Seq
Sorted CD8 T cells from the tumor were collected in RNA later buffer and centrifuged for 5 minutes at maximum speed and RNA was extracted with the RNeasy Plus Micro Kit (Qiagen) following the manufacturer's recommendations. For RNA sequencing, RNA quality was measured with a fragment analyzer. RNA libraries were prepared for sequencing using standard Illumina protocols, Clontech RNA-seq