metabarcoding:1.3PI_A
DNA was extracted from biofilm using PureLink Microbiome DNA Purification Kit, PCR fro rbcL marker, and the purified products were recovered in nuclease-free water and quantified through fluorimetry, DNA ends were repaired using the NEBNext Ultra II End repair enzyme, and the kit labels (EXP-NBD104 and EXP-NBD114) were added to each sample through ligation (SQK-LSK109).