JHSC-0103_RNA
All samples were treated with DNase treatment using DNase I (ThermoFisher, Massachusetts, USA) prior to RNAseq to reduce DNA contamination that might interfere with downstream results. Library generation using the SMARTer Stranded Total RNAseq Kit V3 (Takara Bio USA Inc., California, USA) was performed following the manufacturer's instructions. Final library quantity was measured by KAPA SYBR FAST qPCR and library quality was evaluated using a TapeStation D1000 ScreenTape (Agilent Technologies, CA, USA). Libraries were sequenced on an Illumina NovaSeq instrument (Illumina, California, USA) with a read length configuration of 150 PE for 80M PE reads per sample (40M clusters). Fastq files were quality trimmed using trimmomatic and aligned to the GRCh38 transcriptome using salmon v1.6.0.