GSM7874452: GLO_n_1_NT_chip_ZEB1; Homo sapiens; ChIP-Seq
Cells from one 15-cm dish were cross-linked with 1% formaldehyde at RT for 10 min and quenched in 125 mM glycine for 5 min. Cross-linked chromatin was isolated and sonicated to generate DNA fragments averaging 200-500 bp in length by Bioruptor plus sonication device (Diagenode). Chromatin fragments were immunoprecipitated with antibodies directed against ZEB1 (1 μg, Genetex, GTX105278), or IgG (1 µg, Bio-Rad, PRABP01) as negative control. 5 ng of input and 30 pg of IP were used. Sequencing was performed with Illumina NovaSeq machine with a paired-end protocol (2x75bp, 64Mp reads).