GSM6564284: L02_CTCF_WT_ChIP; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a Bioruptor PICO. ChIP-seq libraries were prepared using the Vazyme DNA Library Preparation Kit complemented with Vazyme DNA Barcodes from Vazyme. 10 ng of DNA was used as starting material for input and ip samples. Libraries were amplified using 13 cycles on the thermocycler. Post amplification libraries were size selected at ~300bp in length using Agencourt AMPure XP beads from Beckman Coulter. Libraries were validated using the Agilent High Sensitivity DNA Kit.