GSM7874731: Mta2_rep1_9462; Mus musculus; ChIP-Seq
Chromatin was extracted from isolated nuclei and sheared to 100-1000bp. Primary antibody was incubated overnight for ChIP reactions. Crosslinks were reversed by adding NaCl and heating at 65 °C for 15 h, RNase A was added and incubated for 2 h at 37 °C, and Proteinase K added and incubated 2 h at 63 °C. DNA was isolated using the MinElute PCR Purification Kit (Qiagen 28004). Libraries were made using NEBNext Ultra II Library Kit (E7645) and sequenced on an Illumina Nova Seq at a depth of 100 million paired end reads.