GSM7845194: Mast cell, non-stimulated 0h, rep2; Homo sapiens; ChIP-Seq
Cells were crosslinked using 1% formaldehyde. Cells were lysed and chromatin was sonicated using a Biorupter Pico (Diagenode). H3K4Me2-marked chromatin was immunoprecipitated using 1 µL of antiH3K4Me2 antibody (ab32356, Abcam). Chromatin-antibody complexes were immobilized on magnetic beads and subjected to tagmentation. Eluted DNA was purified using MinElute spin columns (Qiagen) and amplified for 8-12 cycles using barcoded sequencing primers as per standard ATAC-Seq protocol. Libraries were purified using dual (0.5x-2.0x) SPRI Ampure XP beads (Beckman Coulter) and sequenced on a Illumina HiSeq2500 (Illumina) running a single-read 50 bp protocol.