Prevalence of Mycoplasma hyorhinis Infection Common in Cell Lines
The sequencing library was prepared utilizing the Twist Library Preparation EF Kit With Amp Mix, 96 Samples- 104175. The Sequencing technology used was SBS technology and Paired-end reads were generated on Illumina Novoseq6000 (Illumina Inc.), following standard protocols. Genomic DNA was fragmented using ultrasonication, and adapters were ligated to the fragments. PCR enrichment was then performed to amplify the adapter-ligated fragments. The adapter trimming was performed using the fastq-mcf tool [version: 1.04.803] and reads were de novo assembled using Spades (version. 3.11.1)