Ribo-seq  of Chlamydomonas reinhardtii
library_source: translatome.In brief,  4107 CC-125 cells were harvested by centrifugation at 3 000 g for 3 min, then washed two times with polysome buffer (20 mM Tris*Cl pH 7.4, 150 mM NaCl, 5 mM MgCl2, 1 mM DTT, 100 g/ml cycloheximide), and resuspend in 400 L lysis buffer (Polysome buffer plus 1% Triton X-100 and Turbo DNase I 25 U/ml), the cell paste was flash frozen and pulverized in liquid nitrogen. The frozen powder was thawed on ice and clarified by centrifugation for 10 minutes at 20 000 g at 4C, recover the soluble supernatant and storage at 80C. Next steps including footprint fragment purification, footprint fragment dephosphory and linker ligation, reverse transcription, circularization, rRNA depletion, library construction PCR and sequencing