Bulk soil
The libraries were prepared using Illumina DNA Prep, (M) Tagmentation library preparation kit (Illumina) following the manufacturer's user guide. 20-50 ng DNA was used to prepare the libraries. The samples underwent the simultaneous fragmentation and addition of adapter sequences. These adapters are utilized during a limited-cycle PCR in which unique indices were added to the sample. Following the library preparation, the final concentration of the libraries were measured using the Qubit dsDNA HS assay kit, and the average library size was determined using the Agilent 2100 Bioanalyzer (Agilent Technologies). The libraries were then pooled in equimolar ratios of 0.6nM, and sequenced paired end (2 x 150 bp) for 300 cycles using the Illumina NovaSeq 6000 system