GSM7901094: human adipocyte, ATAC-seq, ZNF189 depletion replicate 1; Homo sapiens; ATAC-seq
Nuclear isolation by hypotonic buffer and mechanical lysis followed by centrifugation in 0.3M sucrose buffer PCR amplification, qPCR to determine additional cycles, and library purification were performed using reagents indicated in the Omni-ATAC protocol (Corces et al., https://doi.org/10.1038/nmeth.4396)