Illumina HiSeq 2500 paired end sequencing of SAMD00450222
TRIZOL-Phenol/Chloroform, Library construction and sequencing for the Illumina HiSeq 2500 was provided as a custom service of Eurofins Genomics K. K. (Tokyo, Japan). The polyA fraction (mRNA) was isolated from total RNA, followed by its fragmentation. Then double-stranded (ds) cDNA was reverse transcribed from fragmented mRNA. The ds cDNA fragments were processed for adaptor ligation, size selection (for 200 bp inserts) and amplification to generate strand-specific cDNA libraries. Prepared libraries were subjected to paired-end 2 x 100 bp sequencing on the HiSeq 2500 platform using HiSeq SBS Kit v4 (Illumina, San Diego, U.S.).