11168 grown in human milk replicate A2
C. jejuni cultures were pelleted by centrifugation and total RNA was extracted using a hot-phenol extraction method and genomic DNA was removed by treating wth RNase-free DNase I. The absence of genomic DNA was confirmed by PCR. Final RNA quality and quantity was ascertained using a Aglient Bioanalyzer. Strand-specific, rRNA-depleted sequencing libraries were generated and sequenced on a Illumina HiSeq 4000 by Genome Quebec. Cutadapt was used to remove potential adapter contamination and ribosomal RNA reads were removed using SortMeRNA prior to data deposition.