GSM6383019: Ctrl-2_ATACseq; Homo sapiens; ATAC-seq
Fresh cells were harvested and resuspended with cold lysis buffer for 10 min on ice. 50,000 nuclei were counted using trypan blue staining and prepared for transposition reactions according to TruePrep DNA Library Prep Kit V2 for illumine (Vazyme, TD501) manufacturer's instructions. Subsequently, nuclei were pelleted and resuspended with transposase at 37 °C for 30 min. After transposition, DNA fragments were purified with AMPure XP beads (Beckman Coulter, A63882). DNA libraries were constructed after 8 cycles of PCR amplification using the TruePrep DNA Library Prep Kit and sequenced on the Illumina Hiseq-PE150 platform to a depth of 4.0 × 107 reads.