GSM6693194: R1_NrasG12DPtenKO_scRNAseq; Mus musculus; RNA-Seq
For each sample the Chromium Controller platform of 10X Genomics was used for single cell partitioning and barcoding. Each cell's transcriptome was barcoded during reverse transcription, pooled cDNA was amplified and Single Cell 3' Gene Expression were prepared according to the manufacturer's protocol “Chromium NextGEM Single Cell 3' Reagent Kits v3.1” (CG000315, 10X Genomics). Both Single Cell 3' Gene Expression libraries were quantified on a 2100 Bioanalyzer Instrument following the manufacturer's protocol “Agilent DNA 7500 kit” (G2938-90024, Agilent Technologies). These Single Cell 3' Gene Expression libraries were combined to create one sequence library pool which was quantified by qPCR, according to manufacturer's protocol “KAPA Library Quantification Kit Illumina® Platforms” (KR0405, KAPA Biosystems). A NovaSeq 6000 Illumina sequencing system was used for paired end sequencing of the Single Cell 3' Gene Expression libraries at a sequencing depth of approximately 17.000 mean reads per cell for Control liver  and 35.000 mean reads per cell for NrasG12DPtenKO.