16S rRNA Seq of stools from mouse
V3 and V4 regions of the 16S rRNA gene were amplified with Invitrogen PlatinumTaq DNA Polymerase and barcoded using the Nextera DNA Library Preparation Kit and primers (F primer: 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG-3', R primer: 5'- GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC-3'). Libraries were constructed and barcoded using the Nextera DNA Library Preparation Kit, normalized to equal concentration, pooled, and sequenced on the Illumina MiSeq.