GSM8005334: ChIP-Seq, MCF-7, TBP, RNase, IP, rep1; Homo sapiens; ChIP-Seq
After reverse cross-linking, RNase A, and proteinase K treatment on MYC-DNA adducts enriched with anti-MYC or anti-TBP antibodies, DNA was purified with phenol:chloroform:isoamyl alcohol (25:24:1, pH 8.0). DNA libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB). 5 ng of DNA was used as starting material for input and IP samples. Libraries were amplified using 9 cycles on the thermocycler. Post-amplification libraries were size selected at 200 - 500 bp in length using Agencourt AMPure XP beads from Beckman Coulter. Libraries were validated using the 1× dsDNA High Sensitivity DNA Kit (Invitrogen).