GSM6383643: K562 PRO-seq As; Homo sapiens; OTHER
Precision Run-On sequencing (PRO-seq) was performed as previously described {Mahat et al., 2016}. Briefly, nuclei were extracted from ~2x107 cells and run-on reactions were carried on at 37°C for 3 minutes using 3ml of each 11-biotin-labelled ribonucleotide stocks (Perkin Elmer). Libraries were prepared as described in Mahat et al., 2016 using custom Illumina small RNA compatible linkers, alkaline degradation, enzymatic treatments and streptavidin selection of nascent RNA on magnetic beads according to the cited protocol.