GSM8415595: A11; Homo sapiens; RNA-Seq
Total RNA from hMSLCs and hpMSC RNA was isolated with Genelute Total Mammalian RNA Kit (Sigma-Aldrich) and cDNA was obtained by using Transcriptor First Strand cDNA Synthesis Kit (Roche). cDNAs were subjected to Illumina TruSeq library preparation (Illumina). Libraries were sequenced on an Illumina HiSeq 2500. RNA-Seq (polyA capture); NGS libraries with polyA capture were prepared according to the manual Protocol for use with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) (version 1.0, 7/20)