GSM6696665: Noncachexia4; Homo sapiens; RNA-Seq
Total RNA was extracted using the mirVana miRNA Isolation Kit (Ambion) following the manufacturer's protocol. RNA integrity was evaluated using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) The libraries were constructed using TruSeq Stranded Total RNA with Ribo-Zero Gold according to the manufacturer's instructions. Then these libraries were sequenced on the Illumina sequencing platform and 150 bp/125bp paired-end reads were generated.