Bacteria_PD1_Bacteroides
One-half murine fecal pellet per mouse was aliquoted into a 96 well PowerBead Pro Plate (QIAGEN) and samples were extracted using the DNeasy 96 PowerSoil Pro QIAcube HT (QIAGEN). Briefly, samples were homogenized in lysis buffer using the TissueLyser II (QIAGEN) before subsequent processing by the QIAcube HT as previously described (Farhadfar et al. 2021) Following fecal DNA extraction, the 16S rRNA gene V3-V4 hypervariable region was amplified using barcoded primer pairs 341F (5 =-CCTACGGGNGGCWGCAG-3 =) and 785R (5 =-GACTACHVGGGTATCTAATCC-3 =) with universal Illumina paired-end adapter sequences.