GSM7664428: hiPSC-derived neuron cells, RTS 2, Day 10, replicate 2; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNAeasy kit (Qiagen) following manufacturer's instructions. Quality, quantification and sizing of total RNA was evaluated using the RNA 6000 Pico assay (Agilent Technologies Ref. 5067-1513) on an Agilent 2100 Bioanalyzer system. The RNA integrity number (RIN) was calculated for each sample and only samples with RIN >9 were kept for further use. Samples were sent to Genewiz (Azenta Life Sciences, Steißlingen, Germany) for high throughput bulk RNA sequencing according to their guidelines for sequencing on an Illumina Novaseq (2x150).