BCG Pasteur ATCC 35734
The genomic DNA was randomly sheared into short fragments. The obtained fragments were end repaired, A-tailed, and further ligated with Illumina adapter. The fragments with adapters were PCR amplified, size selected, and purified. An Illumina library was prepared with the Nextera DNA Flex kit and Nextera DNA CD indexes, and 2150-bp sequencing was performed on a MiSeq sequencer using the MiSeq reagent kit v.3 (Illumina, USA).