GSM7875127: tumor positive, scRNA-seq; Mus musculus; RNA-Seq
Lung tissue collected from the mouse was minced and dissociated for 40 minutes at 37°C using the Tumor Dissociation Kit, mouse (Miltenyi Biotec, Bergisch Gladbach, Germany). The digested tissue was filtered through a 70-μm strainer to obtain a single cell suspension. Total RNA capture and cDNA amplification were performed according to the manufacturer's protocol (Chromium Next GEM Single Cell 5p RNA library v2 protocol, 10x Genomics). Briefly, Single-cell suspensions were loaded onto the 10x Chromium system targeting 7,000 cells. For each single cell, total RNA from the cell lysate contained in gel bead-in-emulsion (GEM) was captured with UMI and 10x barcoded gel bead and then reverse transcribed to cDNA. The barcoded cDNAs were pooled and amplified. Library construction was performed according to the manufacturer's instructions (Chromium Next GEM Single Cell 5p RNA library v2 protocol, 10x Genomics).