Screening downstream target genes for regulating cotton fiber development in the downstream of GhAXR2 and GhSHY2
After the sample is detected to be qualified, the library is constructed, and the main flow is as follows:(1) Enriching eukaryotic mRNA with magnetic beads containing Oligo(dT);(2) Fragmentation Buffer was added to randomly interrupt mRNA;(3) use mRNA as a template, synthesizing a first cDNA chain and a second chain, and purify that cDNA;(4) the purified double-stranded cDNA is subjected to end repair, a tail is added and a sequencing joint is connected, and then the fragment is carried out by using AMPure XP beadsSegment size selection;And (5) finally obtaining a cDNA library through PCR enrichment.