RNA-Seq of Burkholderia seminalis TC3.4.2R3 wild type replicate
The cDNA libraries of Burkholderia seminalis TC3.4.2R3 wild type and the mutant B. seminalis Bsem_02525 Bsem_02526 were obtained (in triplicate) according to manufacturers protocol of Illumina TruSeq RNA Sample Preparation Kit V2 (Illumina, Inc., San Diego, CA, USA). rRNAs were removed from total RNA using Ribo-Zero rRNA removal kit for bacteria (Epicentre Biotechnologies,  Madison, WI).