GSM6561051: ABC multiplex K562 rep3 (originally rep1); Homo sapiens; OTHER
Frozen HEK293 or K562 cell pellets were lysed in 1 mL lysis buffer (50mM Tris pH 7.4, 100mM NaCl, 1% Igepal, 0.1% SDS, 0.5% Sodium Deoxycholate) supplemented with 5 µl protease inhibitor cocktail and 10 µl RNase inhibitor and sonicated for 5 min with 30 second on/off cycles. The lysate was then treated with 10 µl diluted (1:25) RNase I and 5 µl TurboDNase and incubated at 37°C for 5 min. Cellular debris was removed by centrifugation at 12,000 x g for 3 min.