GSM8231278: LOVO, sgCTL, COMBO, 3; Homo sapiens; RNA-Seq
Total RNA was isolated after 16 hours of vehicle or Tazemetostat (5 uM) + Trametinib (50 nM) (Combo)  treatment using the RNeasy Plus Mini kit (Qiagen, #74136). Cells were scraped and collected in RLT plus buffer, homogenized using Qiagen QIAshredder, and gDNA was removed with gDNA eliminator columns. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads.  After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library.  After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library.