16S rRNA gene sequencing of human gut microbiota
Genomic DNA was extracted from stool samples with the MagAttract microbial DNA kit (Qiagen) using a custom automated protocol on the Hamilton Microlab Star and the 16S rRNA gene sequence amplification process performed as previously described (Prochazkova et al. 2021). V3V4 region of the bacterial 16S rRNA gene were amplified with primers 319F and 806R as previously described by (Fadrosh et al. 2014; Holm et al. 2019). High-quality amplicon sequences were obtained on an Illumina HiSeq 2500 modified to generate 300 bp paired-end reads (Holm et al. 2019).