WES of homo sapiens: subject 1 pre-NeoVax PBMC
Exome sequencing libraries were prepared using the NEBNext Ultra II FS DNA library prep kit (New England BioLabs). Target enrichment was first performed by hybrid capture using the xGen Exome Research Panel (#10005153) in combination with the Cancer-Enriched Panels-Tech Access panel (Integrated DNA Technologies). The xGen Exome Research Panel targets 19,433 genes with a total probe coverage encompassing 39 Mb of genomic space. The Cancer-Enriched Panels-Tech Access panel provides an additional 1,855 probes that enrich for cancer-associated gene regions. Libraries were generated using the NEBNext Ultra II FS Kit and paired end 151 base pair reads were sequenced on the NovaSeq6000.