GSM7767462: Thermo neutral, visceral fat, mouse 2; Mus musculus; RNA-Seq
RNA was isolated from mature adipocytes using a combined method of trizol-CHCl3  (Sigma, Cat. C2432) and RNeasy Mini Kit (Qiagen, Cat. 74104) and RNA quality was assessed using an RNA 6000 Nano Kit (Agilent Technologies). mRNA was fragmented and cleaned using RNA Clean XP beads (Beckman Coulter)  and cDNA was generated using a SuperScript III Reverse Transcriptase (Life Technologies, Cat. 18080044), followed by A-tailing and adapter ligation steps performed following the instructions of the manufacturer. The resulting double-stranded DNA (dsDNA) fragments were purified using Agencourt AMPure XP beads (Beckman Coulter, Cat. A63880) and concentration was determined with specific adapters using a Qubit dsDNA High-Sensitivity Kit (Invitrogen, Cat. Q32851).