Shotgun metagenomic sequencing of 18 m depth sample (replicate 2) from Bundera Sinkhole, Australia
~4 L water samples were pre-filtered using 60 m filters (Millipore Type NY60), and then passed through 0.2 m SterivexTM filters. The 0.2 m filters with captured microbial cells were cut from their casing, and DNA extractions carried out using the PowerWater DNA Isolation kit (MO BIO Laboratories, Inc., Carlsbad, USA), according to the manufacturers protocol. Metagenomic libraries were prepared for duplicate biological replicates from each depth using the Illumina Nextera XT DNA Library Preparation Kit, according to the manufacturers protocol, and sequenced on the HiSeq platform (High-Output v4)~4 L water samples were pre-filtered using 60 m filters (Millipore Type NY60), and then passed through 0.2 m SterivexTM filters. The 0.2 m filters with captured microbial cells were cut from their casing, and DNA extractions carried out using the PowerWater DNA Isolation kit (MO BIO Laboratories, Inc., Carlsbad, USA), according to the manufacturers protocol. Metagenomic libraries were prepared for duplicate biological replicates from each depth using the Illumina Nextera XT DNA Library Preparation Kit, according to the manufacturers protocol, and sequenced on the HiSeq platform (High-Output v4)