RNA-Seq of homo sapiens: undifferentiated human induced pluripotent stem cells
Cells were lysed with Trizol (Thermo Fisher) and total RNA was extractedusing a column-based miniprep kit (Zymo). Afterwards, 200 ng totalRNA were transcribed into double-stranded cDNA and amplified by PCR.Sequencing library was generated using the Native Barcoding Kit 24 V14(Oxford Nanopore Technologies) and sequenced on a P2 solo for 72 h.