GSM6384108: Muscle LPS Treated Rep 1; Mus; RNA-Seq
C57BL/6 mice were purchased from Charles River Laboratories (Malvern, PA). C57BL/6 male mice were randomly put into vehicle control, LPS-treated, or LPS-MIC-1-treated group as previously described in GEO (accession no. GSE155738). MIC-1 (80 mg/kg b.w.) was given by gavage daily for 3 days before LPS treatment, which was administered intraperitoneally. Gastrocnemius muscles were collected from euthanized mice after 16 h of LPS treatment. Fat and connective tissue were trimmed from muscle tissues before freezing in liquid nitrogen for further analyses. The handling and care of animals used in this study followed the standard procedures recommended by the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health, and the Rutgers University Institutional Animal Care and Use Committee approved the protocol (ID999900474) RNA was extracted with the RNeasy Mini Kit (Qiagen) according to the manufacturer's protocol.