GSM7295675: WT Col-0 pollen; Arabidopsis thaliana; Bisulfite-Seq
Genomic DNA was isolated pollen or pooled seedlings using the Edwards method (Edwards et al. 1991, NAR 19:1349). Whole genome bisulfite sequencing was performed by BGI Genomics (Hong Kong). Genomic DNA was fragmented by sonication, end-repaired and ligated to methylated adaptors. After bisulfite treatment, bisulfite-treated fragments were PCR amplified and sequenced as paired-end 100bp reads (PE100) with DNBSEQ technology (BGI).