GSM7767316: Barcoding_library_of_PB_1; Mus musculus; OTHER
For CARLIN library preparation from the genomic DNA, the genomic DNA was extracted using DNA Purification Kit (LGC Biosearch Technologies) according to the manufacturer's instructions. Up to 250 ng of gDNA were used for a UMI-tagging reaction with the primer UIM using a single extension step for five cycles. The products were then purified using SPRI beads and then was used for a UMI-tagged CARLIN sequences amplifying PCR reaction (primers NGS2_F, TS1; 14 cycles). After purification with 0.8X SPRI beads, half of the products were used for PCR2 (primer NGS2_F, NGS1_Bulk_R; 14 cycles). Then, the indexing PCR was performed (primers P5, NGS2R_I#; 10 cycles) followed by sequencing on Illumina MiSeq using paired-end 500 cycles v2 kits (250 : 6 : 250) with 10% PhiX sequencing control v3 (Illumina)