GSM6939432: K562 cells, H-mLibB CRISPR/Cas9 library, day10, replicate 1; Homo sapiens; OTHER
Genomic DNA harvested using DNA isolation kit (Tiangen Biotech). All of genomic DNA was used for the construction of sequencing libraries. Library was prepared using the NEBNext Ultra II Q5 Master Mix kit (NEB). 2ug of genomic DNA was used in each 50ul PCR reaction.