Illumina reads of Cas10-Csm complex-associated RNAs in WT Twillingate challenged RP62A
The small RNA were treated with Antartic Phosphatase and PNK and libraries were constructed with the NEBNext Small RNA Sample Prep kit (NEB). The library pool was quantitated by qPCR and sequenced on one MiSeq flowcell for 151 cycles from each end of the fragments using a MiSeq 300-cycle sequencing kit version 2.