Amplicon library from SCOPE PARAGON I PIT trap at 300m
16S rRNA gene amplicons using the Earth Microbiome Project (EMP) protocol and primers for the V4 region. 5 PRIME HotMasterMix was used for amplification. AMPure XP beads were used to purify PCR products. Sequenced with the MiSeq 600 cycle Version 3 sequencing kit.