GSM6825410: WF_939310T; Homo sapiens; RNA-Seq
RNA was extracted from placenta tissue from individual subjects using TRi regaents and cleanup was done using the RNeasy Mini Kit per manufacturer's instructions including on-column DNase digestion. Total RNA was quantified using a sphectrophometrically. Directional cDNA libraries for mRNA-sequencing were prepared using polyA-mRNA from individual RNA samples using Illumina TruSeq reagents. Briefly, 1 μg of total RNA was utilized for mRNA isolation using poly-A magnetic beads and was fragmented prior to first and second strand cDNA synthesis. Libraries were generated via PCR including dual-indexed barcodes. Following purification with AmpureXP beads libraries were quantitated using Qubit dsDNA reagents. Pooled cDNA libraries were sequenced (150 bp paired reads) using a NovaSeq 6000 instrument.