CBASS_hypoxia
Frozen coral fragments were immersed in RLT buffer (Qiagen) within zip-lock bags and the tissue was air-picked from the skeleton using airflow. Total RNA was extracted using the Qiagen RNeasy mini kit with QIAcube Connect using 'standard' protocol. RNA concentrations were assessed using Qubit RNA BR assay kit on the Qubit 4 Quant fluorometer (Invitrogen). RNA quality was evaluated by capillary electrophoresis via the presence of intact 18S and 28S ribosomal RNA bands using the QIAxcel RNA quality control kit v2.0 on the QIAxcel Advanced system (Qiagen). A NovoSeq PE150 RNA prep kit was used to separate the mRNA from the total RNA via polyA enrichment and to generate 2x150bp long paired-end libraries for each sample. Sequencing was performed on the NovaSeq 6000 PE150 sequencer at Novogene, UK.