De novo genome sequencing and assembly of Pseudomonas saxonica NCCB25029
Genomic DNA was subjected to library prep using the SQK-NBD114.24 kit following a multiplex run on a GridION platform and real-time basecalling. Raw reads were QC'd using 'chopper' to select reads of 1100bp and longer with a Q of 10 or higher, thereafter 50bp from head and tail of each read were removed. All reads were then merged into a single FASTQ file for further processing.