GSM8185184: K4me3_cKO_cDC1_1; Mus musculus; OTHER
CUT&RUN:  Unfixed cells (15K) were bound to concanavalin A-coated magnetic beads for 15 min at room temperature, permeabilized with 0.05% digitonin and incubated with primary antibodies overnight at 4°C. After addition of pA-MNase and digestion, bound complexes were released for DNA extraction. Libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina using Multiplex Oligos for Illumina dual index primer pair barcodes purchased from NEB. 6ng of DNA was used for library construction. Libraries were amplified using 12 cycles on the thermocycler. Post-amplification libraries were size selected using 200-600 bp in length using Agencourt AMPure XP beads from Beckman Coulter. Libraries were validated using the Agient High Sensitivity DNA Kit.