16S rRNA V4 region of bird metagenome from Tyto alba sample Tyto alba 028
DNA was extracted from a cloacal swab of the owl. Then the V4 region of the 16S rRNA gene was amplified using the primers 515F and 806R.  Three independent PCRs were performed for each sample, which were then combined. Equimolar amounts of each sample were then pooled before sequencing on a 150 bp paired-end run of an Illumina HiSeq.