EM-seq: Persisting epimutations PGCLC con rep 3
Genomic DNA was extracted from cells using phenolchloroform extraction and smaller fragmented DNA ( 10 kb) and contaminating RNA were removed by cleaning the genomic DNA on a genomic DNA clean and concentratorTM-10 column (D4011), according to the manufacturers instructions (Zymo Research, CA USA). Large size (470-520 bp) EM-seq libraries were prepared with the NEBNext Enzymatic Methyl-seq kit (E7120S) according to the manufacturers protocol (New England Biolabs, MA USA).