RNA-Seq zebrafish GFAP:GFP
mRNA was isolated from 0,5 g of total RNA using poly-T oligo-attached magnetic beads and using two rounds of purification (positive selection) as suggested in the TruSeq Stranded mRNA Reference Guide (Illumina #1000000040498). Purified samples were processed using TruSeq Stranded mRNA Library Preparation Kit (Illumina #20020594)