GSM6595253: Endometriosis Patient 3 - Ectopic Adjacent; Homo sapiens; RNA-Seq
Single cells were collected through cold active protease dissociation protocol, viable cell sorting strategy. 12,000 single cells were loaded into one lane of a 10X Genomics Chromium controller, targetted 6,000 barcoded single cells. Single cell capture, barcoding and library preparation were performed using the 10X Chromium platform, version 3 chemistry. cDNA and libraries were checked for quality on Agilent 4200 Tapestation, quantified by KAPA qPCR, and sequenced on an Illumina NovaSeq 6000 (either an S2 or S4 flow cell) targetting 100,000 raw read pairs per cell.  For Cell Hashing libraries, cells from each input sample were labeled with BioLegend TotalSeq-A anti-human hashtags prior to loading onto the 10X Genomics Chromium system and were sequenced by pooling the gene expression and hashtag libraries at a 20:1 ratio before sequencing on an Illumina NovaSeq 6000, targetting 100k read pairs per cell. For Bulk libraries, fresh tissue were either preserved in RNA Later or flash frozen before extraction. Frozen tissues were submitted to the Genome Technologies core service at the Jackson Laboratory for RNA extraction, and sequenced on NovaSeq6000 (Illumina) with SP 100 cycles single-end reads kit resulting in, on average, 42.7 million reads per sample. 10X Genomics Chromium v3 chemistry