GSM7017763: CIA+JAKi synovial cells scRNA-seq; Mus musculus; RNA-Seq
Fresh synovial tissue was dissociated in type II collagenase solution (Worthington, 4 mg/mL) at 37 °C for 1 hour with frequent shaking. After the first 30-min incubation, cell suspensions were carefully homogenized by a series of 18G, 21G and 25G needles until the cell suspension passed through the needles smoothly. After digestion, cells were collected by centrifugation and the cell pellet was then treated with ACK buffer (Sigma) to lyse the red blood cells, and then filtered using 100-μm cell strainers to remove the tissue debris to get the single-cell suspensions. Sequencing libraries were generated using a 10x Genomics Single-cell 3' Solution (v.3) kit and then subjected to Illumina sequencing (HiSeq 4000)