GSM6634191: R2_Input_siNT; Homo sapiens; ChIP-Seq
Cells were fixed with 0.5% formaldehyde at RT for 10 min then quenched with 150 mM glycine at room temperature, 5 min. Cells were harvested by scraping in cold 1X PBS and pelleted at 1000 x g, 5 min at 4°C. Harvested cells were lysed for 30 min at 4°C in Farnham lysis buffer (5 mM PIPES, 85 mM KCl, 0.5% NP-40, 1 mM PMSF, 1X protease inhibitor cocktail (PIC)). Additional details of Dna shearing, and IP are given in Devlin et al. 2022. For ChIP-seq library preparations, 4 ng of ChIP or input DNA was processed using the KAPA Hyper Prep Kit according to the manufacturer's protocol (Roche KK8504).