PacBio CLR Sequencing of Acropora palmata sperm DNA
Genomic DNA was extracted using Nucleon Phytopure DNA extraction kit (Cytiva, Marlborough, MA) with the addition of RNase treatment and increased incubation time of 3 to 4 h at 65 C during the cell lysis step. Genomic DNA elutions from multiple sperm aliquots were combined and concentrated using the AMPure bead clean-up (final gDNA= 10 g for A. palmata). PacBio libraries were prepared using a 20kb size-selection protocol and sequenced on Sequel II by the Genomics Core Facility at Pennsylvania State University.