Metatranscriptome of Chrysochromulina tobinii -P3 (Non-axenic) Light 5 hrs Rep A
The total RNA was assessed using the Agilent 2100 Bioanalyzer to determine the RNA integrity and RNA samples having RNA Integrity Number (RIN) between 9  10 were used in this work.  Ribo-Zero rRNA removal Kit (cat#MRZY1324) was used for enrichment of transcripts. The SOLiD Total RNA-Seq Kit (cat#4445374) was used to construct template cDNA for RNA-Seq following the protocol recommended by Applied Biosystems. Briefly, mRNA was fragmented using chemical hydrolysis followed by ligation with strand specific adapters and reverse transcript to generate cDNA.  The cDNA fragments, 150 to 250 bp in size, were isolated and amplified through 15 amplification cycles to produce the required number of templates for the SOLiD EZ Bead system, which was used to generate the template bead library for ligation base sequencing by 5500xl SOLiD instrument (LifeTechnologies).