GSM7666898: 92863, BFoxm1, scRNA-seq; Mus musculus; RNA-Seq
Mouse islets were isolated by collagenase digestion, followed by Ficoll gradient and handpicking The Single Cell 3' Reagent Kit v2 or v3 was used for generating scRNA-seq data. All libraries were validated for quality and size distribution using a BioAnalyzer 2100 (Agilent) and quantified using Kapa (Illumina). For samples prepared using 'The Single Cell 3' Reagent Kit v2', the following chemistry was performed on an Illumina HiSeq4000: Read 1: 26 cycles, i7 Index: 8 cycles, i5 index: 0 cycles, and Read 2: 98 cycles. For samples prepared using 'The Single Cell 3' Reagent Kit v3', the following chemistry was performed on an Illumina HiSeq 4000: Read 1: 28 cycles, i7 Index: 8 cycles, i5 index: 0 cycles, and Read 2: 91 cycles.