GSM7932194: HAR123-KO, ESC, Rep3; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNeasy Plus Mini kit (Qiagen), following the manufacturer's protocol. Ribosomal RNAs were removed using NEBNext® rRNA Depletion Kit v2 (New England Biolabs). Purified RNAs were used to make the library using the NEBNext Ultra II Directional RNA Library Prep kit (New England Biolabs), as per manufacturer's instructions. Libraries were sequenced (pair-end reads) with an Illumina NovaSeq 6000 platform for 100 cycles at the UCSD institute for Genomic Medicine (IGM) core.