GSM7873786: Chlamydomonas_Nanopore_dRNAseq; Chlamydomonas reinhardtii; RNA-Seq
After extracting total RNA from the samples using TRIzol (Thermo Fisher Scientifific, 15596026), mRNA was isolated via a Dynabeads mRNA Purifification Kit (Thermo Fisher Scientifific, 61006) with genomic DNA removal using TuRBO DNase kit (Thermo Fisher, AM2238). Nanopore direct RNA sequencing was conducted following instructions provided by Oxford Nanopore Technologies (Oxford, UK) using DRS kits (SQK-RNA002) and MinION flowcells (FLO-MIN106D, R9.4.1 pore).