Toc HiC 1
For Hi-C library construction, the Mbol restriction enzyme was used to digest the cross-linked high molecular weight (HMW) gDNA. After 5' overhang biotinylated and blunt-end ligation, the DNA was physically sheared into 300500 bp fragments. Finally, the Hi-C library was sequenced with a strategy of 2150bp on the Illumina HiSeq 2500 platform.