GSM8185735: Brain, WT LPS x2, replicate 2; Mus musculus; RNA-Seq
Mice were transcardially perfused with ice-cold PBS and the brain tissues were freshly collected. The brain tissues were then enzymatically dissociated using Adult Brain Dissociation Kit (Miltenyi Biotec) to obtain brain single cell suspensions. Libraries were prepared using Chromium controller according to the 10x chromium Next GEM Single Cell 3' v3.1 protocol (CG000315). Briefly, the cell suspension was mixed with master mix and loaded with Single Cell 3'V3.1 Gel Beads and Partitioning Oil into a chromium Next GEM chip G. RNA transcripts from single cells were uniquely barcoded and reverse-transcribed within droplets. cDNA molecules were pooled and were subjected to repair process. The purified libraries were quantified using qPCR according to the qPCR Quantification Protocol Guide (KAPA) and qualified using the Agilent Technologies 4200 TapeStation (Agilent technologies).