Characterization of the complete mitogenome of Pseudomphala latericea (Littorinimorpha: Truncatelloidea: Assimineidae) and phylogenetic relationships
After the sample genome DNA is tested to be qualified, break the DNA with enzymes, and then purify the broken DNA to build a sequencing library. The steps are as follows: repair the DNA end, add A to the 3 'end, break the DNA, and then purify the broken DNA to build a sequencing library. The steps are as follows: repair the DNA end, add A to the 3' end, connect the sequencing connector, recover the target segment by agarose gel electrophoresis, and PCR amplification of the target segment, Finally, a sequencing library was built. Before sequencing, perform quality inspection on the constructed library. After passing the inspection, use Illumina NovaSeq6000 platform for sequencing