GSM6634208: A673 SCR, IgG (CST); Homo sapiens; OTHER
Cells were adsorbed onto activated Concavalin A beads and then incubated with antibodies O/N at 4°C. pAG-MNase was added to each sample to produce chromatin fragments under targets. Fragmentation was terminated with the addition of STOP buffer (340 mM NaCl, 20 mM EDTA, 4 mM EGTA, 50 ug/mL RNaseA, 50 μg/mL Glycogen) and 0.5 ng/ul E.coli spike-in DNA. DNA was purified using a column purification kit and used for library assembly. CUT&RUN libraries were assembled using the NEBNext II Ultra Library Prep kit and dual-index primers following manufacturer protocols