Comparing Stability of Fecal Microbiota by Time and Weather Conditions in a Carnivore, the Cheetah (Acinonyx jubatus)
Each sample PCR in duplicate with 515F, 939R primers using two-step library prep, adding in-house i5 and i7 indexes. Cleaned with spri beads. Each sample quantified using Qubit. Samples pooled equimolar. Sequenced on two miseq runs (run one with _, run two with -)