GSM7299698: LiHe_MO4_1892; Mus musculus; Bisulfite-Seq
liver hepatocytes isolated by perfusion. RRBS protocol using restriction enzyme MspI DNA was fragmented with MspI restriction enzyme. Fragments were end-repaired and A-tailed, followed by NGS adaptor (methylated) ligation using T4 ligase. Fragments were then bisulfite converted and amplified by PCR.