GSM7665689: cKit+Mac1+ cells, Enl-T1, Rep1; Mus musculus; RNA-Seq
RNeasy UCP Micro kit (Qiagen, #73934) was used to extract total RNA and remove genomic DNA. 500ng total RNA was used for library preparation using polyA mRNA magnetic Isolation module (NEB, #E7490) and RNA library prep kit (NEB, #7770) following manufacturer's instructions. The library quality was assessed on an Agilent Bioanalyzer DNA chip (Agilent, #5067-1504). RNA-seq samples were sequenced using Illumina NextSeq 500.