GSM7017566: Control rep 1; Homo sapiens; RNA-Seq
Total RNA was harvested using Qiazol (Qiagen), purified from DNA contamination through DNase I (Qiagen) and enriched by  RNeasy (Qiagen) columns for gene expression profiling. Quantity and integrity of the extracted RNA were assessed by NanoDrop Spectrophotometer (NanoDrop Technologies, DE) and by Agilent 2100 Bioanalyzer (Agilent Technologies, CA), respectively. RNA libraries for sequencing were generated  using 500ng of RNA for each sample according to the Illumina TruSeq Stranded Total RNA kit with an initial ribosomal depletion step using Ribo Zero Gold (Illumina, CA). Libraries were quantified by qPCR and sequenced in paired-end mode (2x75 bp) with NextSeq 500 (Illumina, CA).