GSM7299691: 4T1, ARIH1-C355S-4-OE; Mus musculus; RNA-Seq
Total RNA was isolated using a TRIzol total RNA extraction kit (TIANGEN,Cat.No.DP424),which yielded > 2 μg of total RNA per sample. RNA quality was examined by 0.8% agarose gel electrophoresis and spectrophotometry.High-quality RNA with a 260/280 absorbance ratio  of 1.8-2.2 was used for library construction and sequencing. Illumina library construction was performed according to the manufacturer's instructions (Illumina,USA).