GSM8173197: LysM-Cre x R26-stop-EYFP x Tgfbr2-fl/fl inflammatory cardiac macrophages biol rep 4; Mus musculus; RNA-Seq
RNA was isolated using Total Genomic DNA Isolation Kits (Norgen Biotek Corp., Thorold, Canada) following the manufacturer's protocol The mRNA samples were initially fragmented, and double-stranded cDNA synthesis was performed. The 5' end of the cDNA was phosphorylated, and a polyA tail was attached to the 3' end for PolyA selection rRNA removal