GSM7665821: EO771_Isotype_lane1, HTO; Mus musculus; OTHER
Mouse tumors were enzymatically dissociated to generate singe cell suspensions and enriched for immune cell populations using CD45 MicroBeads (Miltenyi). Individual samples from each mouse were hashed using Total-seq-c antibodies (Biolegend). For each treatment, 6 mice were pooled together, and 40,000 cells were loaded into each channel of Chromium single-cell 5' Chip to create a single cell GEM using the Chromium X machine. cDNA and the subsequent libraries were prepared according to the manufacturer's recommendations. Libraries from two 10x channels were pooled and sequenced together using Illumina NovaSeq S1. Library was constructed according to 10x genomics manufacturer instructions for 5' chromium kit