MiSeq of vegetable endophytic bacterial communities: vegetable farms
The hypervariable V4 region of 16S rRNA library was constructed by following Illumina MiSeq protocol. Briefly, the protocol included a first stage amplicon PCR step involving 0.2 M concentrations of primers 341F (forward) and 805R (reverse). Sequences were demultiplexed and depleted of Illumina barcodes and primers by using MiSeq reporter and Trimmomatic software