GSM8139137: DIV12 primary neurons, A300, CUT&TAG; Mus musculus; OTHER
In brief, primary neurons were collected and bounded to Concanavalin A-coated beads. Subsequently, cells were resuspended in antibody buffer and incubated with primary antibodies against menin antibody (A300-105A; Bethyl) and secondary antibodies in order. The samples were incubated with pA-Tn5 transposase. Aftertransposon activation and tagmentation, DNA was isolated, amplified, and purified to construct library, The library for sequencing was constructed and VAHTS DNAClean Beads (N411, Vazyme Biotech) were used for purification steps. The librarywas quantified with VAHTS Library Quantification Kit for lllumina (VazymeBiotech) and sequenced on an lllumina novaseq 150PE. The sequencing was carried out by Novogene (Novogene, Beijing, China).