RNA-Seq of third-instar larva of Metisa plana treated with farnesyl acetate at 240 mg/L
Third instar larvae samples was collected from an oil palm plantation in Jengka, Pahang, Malaysia. The larvae were treated with farnesyl acetate using leaf-dip method at 240 mg/L. After 7 days, 30 surviving larva were collected and flash frozen using liquid nitrogen. Total RNA was extracted using RNeasy mini kit (Qiagen) and genomic contamination was removed using DNA-free DNA Removal Kit (Invitrogen). cDNA was then constructed using NEBNext UltraII Directional RNA Library Prep Kit for Illumina (BioLabs) and sequenced using Illumina HiSeq 4000 at 150bp paired-end(Novogene)