Genome sequencing of H. pylori strains cultured under regular or high salt
DNA samples were prepared from H. pylori with the Wizard genomic purification kit (Promega). H. pylori DNA samples were subjected individually to enzymatic fragmentation using the NEBNext dsDNA Fragmentase kit (NEB) according to the manufacturers instructions, with average fragment length of 600 bp (range of 4001,000 bp). Libraries of DNA were prepared from purified fragmented DNA samples using the Jetseq library prep kit with unique indexes as per the manufacturers protocol (Meridian Biosciences, Inc. Cincinnati, Ohio, USA). Quantification of these library preps was performed with the Jetseq library quantification kit (Meridian Biosciences, Inc. Cincinnati, Ohio, USA), and the libraries were sequenced on a MiSeq sequencer using the 600V3 kit (Illumina Inc., San Diego, USA).