Union Glacier 16S rRNA gene metabarcoding of bacteria from Rossman cove cultivated in LB agar replicate 2
Two differents culture media with 2.5 g/mL of amphotericin B were used to obtain the culturable fraction of bacteria from each soil sample. Specifically, Luria Bertani (LB) was used as a rich nutrient medium for influencing the growth of microbial strains, and Soil Extract Medium (SEM) was used as a minimum culture medium. The soluble fraction of each soil was used as inoculum for the cultures. The soluble fraction of each soil was used as inoculum for the cultures. The V3-V4 region of 16S rRNA gene (primers 341F 5'-CCTAYGGGRBGCASCAG-3' and 806R 5'-GGACTACNNGGGTATCTAAT-3') was amplified and sequenced using the Illumina NovaSeq system.