GSM8184642: A7r5 cells, siRNALemd3, replicate 2; Rattus norvegicus; RNA-Seq
Total RNA was isolated using TRIzol reagent (Vazyme Biotech Co., Nanjing, China). Total RNA (1 µg) was reverse transcribed into cDNA using a reverse transcription system (Vazyme Biotech Co., Nanjing, China). RNA libraries for RNA-seq were prepared using VAHTS Universal V10 RNA-seq Library Prep Kit for Illumina following manufacturers's protocols.