IgA deficiency destabilizes immune homeostasis towards intestinal microbiota and increases the risk of systemic immune dysregulation
DNA was extracted using DNAeasy PowerSoil HTP 96 kit for the fecal samples. The V4 variable regions of the bacterial 16S rRNA gene were amplified. Purified products were pooled in equal amounts and sequenced on the Illumina MiSeq. The paired-end sequencing protocol was used, yielding 250bp reads in the forward and reverse directions.