GSM6387657: IMR32_H3K27me1; Homo sapiens; ChIP-Seq
ChIP assays were performed as previous papers (Orlando V et al., 1997, Methods, Fujimura Y et al., 2006, Development). Cross-linked chromatin prepared from the indicated cells was precipitated by each antibodies. NEBNext Ultra DNA Library Kit for Illumina (NEB) was used to generate libraries from samples. All procedures were performed according to the protocols suggested by the manufacturers. The concentration of libraries was measured by Qubit 2.0 Fluorometer with Qubit dsDNA HS Assay Kit (Invitrogen). Average length of libraries was analyzed on TapeStation with High Sensitivity D1000 ScreenTape (Agilent Technologies) according to the manufacturer's protocol. Finally, the molar concentration of each library was quantified by Library Quantification Kit (Takara). Single-read sequencing of libraries with a read length of 75 was performed with NextSeq 500 Sequencing System (Illumina)