GSM7295400: BVSC_NRF1_1; Mus musculus; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, ChIP was performed  using a SimpleChIP® Plus Enzymatic Chromatin IP Kit (Cell Signaling Technology, #9005) . Immunoprecipitated DNA were quantified using a Qubit 3.0 Fluorometer (Invitrogen) and qualified with an Agilent Bioanalyzer 2100 (Agilent Technologies). The  ChIP product was treated with End Prep Enzyme Mix for end repairing, 5' Phosphorylation and dA-tailing in one reaction, followed by ligation to adaptors with a “T” base overhang. Each sample was then amplified by PCR using P5 and P7 primers. The PCR products were cleaned up using beads, validated using an Agilent 2100 Bioanalyzer, and quantified by Qubit 3.0 Fluorometer.