GSM6939800: 630BMOB_2; Clostridioides difficile; RNA-Seq
Cells were harvested at an OD of 0.6 by centrifugation for 2 min at 4°C. The cells were first broken by shaking in a Fastprep apparatus for 2 x 30 sec in the presence of one gram of 0.1-mm diameter glass Sigma beads, then treated with Trizol reagent, chloroform/ isoamylalcohol and precipitated with isopropanol. The pellet was resuspended in TE RNA libraries were prepared for sequencing using standard Illumina protocols Illumina stranded Total RNA prep Ligation kit with RiboZero Plus