GSM6564264: NT_LI_ILC2_r3; Mus musculus; RNA-Seq
RNA was harvested using Trizol reagent. RNA was prepared from approximately 10,000–50,000 cells by using TRIzol (Thermo Fisher Scientific Inc.). Total RNAs were subsequently processed to generate an mRNA-seq library using a NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490S), NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads (NEB, E7765S) and NEBNext Multiplex Oligos for Illumina (Index Primers Set 1 and 2) (NEB, E7335S and E7550S). RNA libraries were prepared for sequencing using standard NEB protocols