GSM7845200: CD11b+ cells, WT iGB6, mouse 770 (replicate 2); Mus musculus; RNA-Seq
CD11b+ cells were sorted from the spleens of IgHMOG iGBGFP and WT iGB6 mice 8 days after iGB-T cell co-transfer and total RNA extracted using the RNeasy Micro Kit (Qiagen) according to the manufacturer's instructions without performing on-column gDNA digestion cDNA synthesis and library preparation were performed using NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (New England Biolabs) according to the manufacturer's instructions. Briefly, 8-16 ng RNA was used for cDNA synthesis and the double-stranded cDNA was amplified (17 cycles) and purified using SPRI®select beads (Beckman Coulter). 20 ng cDNA was tagged and ligated with 25-fold-diluted NEBNext® Adaptors for Illumina. The products were bead-purified and the adaptor-ligated DNA was amplified by PCR (9 cycles) using NEBNext® Single Index Primers to generate multiplexed sequencing libraries. Library quality was assessed on an Agilent 2100 Bioanalyzer using the DNA 1000 Kit (Agilent).