GSM8139438: RNA-seq_hFOB 1.19, differentiated 5 days at 39.5⁰C, rep2; Homo sapiens; RNA-Seq
We isolated total RNA from hFOBs using TRIzol reagent (Invitrogen) following manufacturer instructions and depleted it of rRNA utilizing the Ribo-Zero rRNA Removal Kit (Illumina). We prepared RNA-seq libraries with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) following standard protocols. We sequenced libraries on one S2 flow cell on an Illumina NovaSeq 6000, generating ~200 million paired-end 50 bp reads per sample.