GSM8184964: RNAseq_RS_S12NSUV2_DMSO_rep3; Mus musculus; RNA-Seq
Total RNA was extracted from fresh cell pellets in biological triplicates using RNeasy Plus Mini kit (Qiagen). RNA integrity was assessed on TapeStation 2200 (Agilent Technologies) using High Sensitivity RNA Screentape assay, and RNA was quantified with Qubit RNA HS assay kit Libraries of mRNA were prepared using Illumina TruSeq RNA Sample Prep v.2 kit according to the manufacturer's instructions using 0.5µg input RNA. Libraries were quantified with Qubit dsDNA HS assay kit, and size distributions were analyzed on a Tape Station 2200 (Agilent Technologies). Libraries were pooled based on molar concentrations, denatured, loaded onto an Illumina Nextseq 550 with SE75 high-output kits, and sequenced as paired-end 36/36 cycles.