Microdochium nivale F00608 genome sequencing (short Illumina reads), replication 1 R1
DNA was extracted from M. nivale mycelium using the phenol-chloroform method. DNA samples were treated with 100 g/mL RNase A (Qiagen, Germany) and 50 g/mL proteinase K (Qiagen, Germany) (Maghini et al., 2021). Polysaccharide impurities were removed from the DNA samples with cetyltrimethylammonium bromide ( (Ges-Neto, Loguercio-Leite, Guerrero, 2005). The quantity and quality of the DNA samples were assessed using an Implen nanophotometer (Implen, Germany) and 1% agarose gel-electrophoresis, respectively. For Illumina sequencing, the DNA was fragmented using an M220 focused-ultrasonicator (Covaris, USA) . DNA libraries were prepared using the NEBNext Ultra II kit (New England Biolabs, UK). The efficiency of DNA fragmentation and the quality of DNA libraries were estimated using an Agilent 2100 Bioanalyzer and a High Sensitivity DNA kit (Agilent, USA). The sequencing was performed on the Illumina HiSeq 2500 platform at the Joint KFURiken Laboratory, Kazan Federal University (Kazan, Russia).