Nymphal ticks infected with Ehrlichia muris 48 hours after attachment to the C57BI/6 mouse
Three (3) individual replicates per time point (per sample ID)- Process (individually) using RNeasy micro kit (Qiagen)- Reconstituted with 10uL +4uL (total 14uL)- Check RNA concentration (and record) with 1uL- Keep RNA cold until take to the NSG core (ideally), if not possible, freeze immediately in -80C. It was a paired-end 75 base sequencing run. Libraries were prepared with Illuminas TruSeq Stranded RNA kit.