GSM8183022: Kelly transfected piR-1254 1; Homo sapiens; RNA-Seq
RNA was harvested 48 h after transfection with the Rneasy mini kit (Qiagen). 250 ng of total RNA was used for the construction of sequencing libraries. Library preparation and RNAseq were carried out as described in the Illumina “Stranded mRNA Prep Ligation” Reference Guide, the Illumina NextSeq 2000 Sequencing System Guide (Illumina, Inc., San Diego, CA, USA), and the KAPA Library Quantification Kit - Illumina/ABI Prism (Roche Sequencing Solutions, Inc., Pleasanton, CA, USA).