GSM8310807: 5839.P48Cre.AcutePanc RNA; Mus musculus; RNA-Seq
Pancreas was taken at time of euthanasia, minced in RNAlater and snap frozen with liquid nitrogen. RNA extraction was completed with ToTALLY RNA Kit (Ambion, Cat# AM1910) according to manufacturer's protocols. Precipitated RNA was cleaned up according to Qiagen's protocol on the miRNeasy column (Qiagen, Cat# 217004), with an on-column DNA digestion and RNaseOUT (Invitrogen, Cat# 10777019) added to final elution to reduce degradation during storage at -80°C. Illumina TruSeq RNA v2 library preparation