RNA-seq of P. aeruginosa PA1432 sample 1
Strains were growing equally as described for c-di-GMP quantification. At OD600=0.5, cells were harvested from 2 mL of culture via transfer to a centrifugation tube and spinning down at 4,000 g for 5 min at 4C. The cell pellet was immediately flash-frozen in liquid nitrogen and stored at -80C until further use. RNA was extracted and quality (RNA concentration, RIN value,28S/18S, and the fragment length distribution) was validated by Agilent 2100 Bioanalyzer (Agilent RNA 6000 Nano Kit). Library construction, sequence filtering, mapping, and genome expression analysis were performed (BGI, Hong Kong).