GSM8184479: NSC-34 cells transfected with SOD1 WT 48 hrs; Mus musculus; RNA-Seq
We collected the cell lysates with RIPA buffer at 24- and 48-hour timepoints post-transfection. RNA was extracted using the RNeasy mini kit (Qiagen) and total RNA concentration was determined using NanoDrop OD readings at 260/280 wavelengths. Thee samples were then sent for RNA-seq analysis (Novogene, Beijing, China).