Metagenomic DNA from ruminal fluid from calf
DNA from rumen fluid of calf supplemented or not with 200g of coconut oil was extracted with the ZymoBIOMICS DNA miniprep kit; Bacterial 16S Ribosomal RNA genes targeted sequencing was performed using Quik-16S NGS Library Prep Kit (Zymo Research, Irvine, CA). The bacterial and archaeal V3-V4 region of 16S gene was amplified using Zymo's specific primers. The final library was sequenced on Illumina NextSeq with a P1 reagent kit (600 cycles) with 30% PhiX spike-in.