GSM6561064: FMD 7; Mus musculus; RNA-Seq
Total RNA was extracted using RNeasy Mini kit (QIAGEN) followed by on-column DNase I digestion as per manufacturer's instructions. RNA integrity was verified with a Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). RNA was quantified with the Qubit fluorometer (Invitrogen), and 1 µg of purified RNA was utilized as starting material for library construction RNA libraries for RNA-seq were prepared using the KAPA RNA HyperPrep Kit with RiboErase (KAPA BIOSYSTEM KK8560) as per manufacturer's instructions