WES of LuCaP189.3 metastasis
DNA was extracted from tissue using the Qiagen FFPE DNA kit. 1ug of DNA was sheared and assembled into whole exome sequencing libraries using the Agilent SureSelect v6 Human All Exon Kit. Samples were sequenced on the Illumina platform. Pass-filter FASTQ files were trimmed using Trimmomatic, aligned with BWA-MEM, duplicate-marked and quality-score recalibrated using GATK version 4.