RNA-seq of patients with severe fever with thrombocytopenia syndrome in Shandong
Adaptor sequences and low-quality bases were removed from raw sequencing reads by the AfterQC program (version 0.9.6). For ethical reasons, human reads were subsequently removed by mapping against the entire human reference genome (GRCh38) using the HISAT2 (version 2.2.1) program. For de novo assembly, trimmed and high-quality reads were assembled into contigs using Trinity (version 2.12.27). All severe fever with thrombocytopenia syndrome virus related reads were extracted by mapping against the severe fever with thrombocytopenia syndrome virus genomes obtained in this study.