GSM7901022: non-coculture cells (Separately cultured cells)-RNA; Homo sapiens; RNA-Seq
Total RNA was extracted in TRIzol (Invitrogen) and DNase I treated using Directzol RNA Miniprep kit (Zymo Research) according to the manufacturer's instructions. scRNAseq libraries were prepared using the Chromium Next GEM Single Cell 3' v3.1 (Dual index) according to the manufacturer's instructions.