Short Read Illumina 16S Sequencing of Nasal Swab from Chronic Rhinosinusitis Patients
DNA extraction was performed using the QIAGEN DNeasy PowerSoil Pro kit(QIAGEN, Hilden, Germany) at the Australian Genomic Research Facility(AGRF, Melbourne, Australia). Extracted DNA from a common swab was divided and allocated to three separate sequencing pathways: fungal ITS; bacterial 16S (V3-V4) NGS and full length 16S. ITS and 16S NGS amplification and sequencing steps were performed by AGRF in accordance with local protocols (Supplementary 2), applying a 300-base pair chemistry, 2-stage PCR and sequencing utilising Illumina Miseq(Illumina Inc., San Diego, California).