Single-Cell RNA Sequencing of Patient 3 Baseline Psoriasis Lesional skin.
Full thickness skin biopsies were incubated in 5U/mL dispase at 4°C overnight. Dermis and epidermis were dissociated into single cell suspensions separately. Single cell suspensions were incubated with TotalSeq hashtag antibodies (catalogue numbers 394461, 394643, 394647 and 394647 for lesional dermis, lesional epidermis, non-lesional dermis and non-lesional epidermis, resepectively). Following sorting of viable cells on a FACSAria flow cytometer (BD), dermal and epidermal suspensions were mixed at a 1:1 ratio. Libraries were prepared with the Chromium Single Cell 3' kit (10X Genomics) and sequenced to generate 150 base pair paired end reads.