Whole Genome Sequencing of Aspergillus fumigatus clinical isolate DI20-109
Genomic DNA was quantified using the Quant-iT PicoGreen ds DNA assay (ThermoFisher). Genomic DNA was sheared on an LE220 ultrasonicator (Covaris). Libraries were prepared from sheared DNA with the HyperPrep Library Preparation Kit (Roche PN 07962363001). Libraries were analyzed for insert size distribution using the 2100 BioAnalyzer High Sensitivity kit (Agilent), 4200 TapeStation D1000 ScreenTape assay (Agilent), or 5300 Fragment Analyzer NGS fragment kit (Agilent). Libraries were quantified using the Quant-iT PicoGreen ds DNA assay (ThermoFisher) or by low pass sequencing with a MiSeq nano kit (Illumina). Paired end 150 cycle sequencing was performed on a NovaSeq 6000 (Illumina).