GSM8389860: 2209_FB; Mus musculus; OTHER
Back skin was harvested and processed to obtain single-cell suspensions. Samples were barcoded with Oligo-tagged hashtag antibodies (Biolegend) and incubated with an antibody cocktail of Fixable viability dye eFluor780 (eBioscience), CD45-BUV395, CD11b-BV650, Ly6G-PE, EPCAM-APC, mFESK-4-APC, and CD31-APC. Live neutrophils (CD45+CD11b+Ly6G+), other immune cells (CD45+Ly6G-), and structural cells (CD45-Dump+) were isolated by FACS-based sorting. Library preparation (Chromium Next GEM Single Cell 3' GEM v3, 10X Genomics) and the subsequent sequencing (Novaseq 6000 instrument, Illumina) were performed by the Yale Center for Genome Analysis (YCGA), according to the manufacturer's instructions (10X Genomics).