Metagenome of rotten Fomes fomentarius fruiting body
Total genomic DNA was extracted from 200 mg of homogenized frozen material described above using the NucleoSpin Soil Kit (Macherey-Nagel, Germany) following the manufacturers instructions. Briey, cells were lysed using SL1 lysis buer with Enhancer SX added prior to lysis. The samples were homogenized using a FastPrep-24 (MP Biomedicals, Santa Anna, United States) at 5 m.s1 for 2  30 s. Two extractions per sample were performed and pooled. The metagenomic sequencing library was prepared using TrueSeq Nano DNA library preparation kit (Illumina) according to the manufacturers protocol and sequenced in-house on the Illumina MiSeq (2  250 bp).