GSM7665211: Human induced pluripotent stem cells (hiPSCs) from newborn fibroblasts, HD2.3; Homo sapiens; RNA-Seq
Total RNA samples were extracted using miRNeasy Mini Kit (Qiagen) and checked for integrity by microcapillary electrophoresis on 2200 TapeStation instrument (Agilent Technologies). Poly-A+ RNA-seq libraries were prepared using the TruSeq Stranded mRNA Library Prep Kit (Illumina), according to manufacturer's instructions, and sequenced on HiSeq2500 platform (Illumina) in a 125-cycle paired-end run.