RNA-seq of Drosophila yakuba accessory gland tissue
RNA was extracted from pooled accessory glands of 30 unmated, two day old males at 26 C using TRIZOL (Invitrogen) followed by on column cleanup with DNAse digestion (Zymo). RNA libraries were prepared with Illumina Truseq stranded mRNA kit (Illumina), which uses polyT beads to capture polyadenylated RNA sequences. Libraries where sequenced using 150 bp paired-end reads on an Illumina Hiseq4000 at the UC Davis genome center.