GSM7665567: T1_DAPI 2; Arabidopsis thaliana; RNA-Seq
All steps were done on ice, and with the RNase inhibitors. 300 apices were dissected and immersed in 3% glyoxal. The samples were vacuumed for 10 min twice and washed with Galbraith buffer. Next, the samples were grounded by Bioruptor  for 1 min and debris was filtered. The mixture was subjected to centrifugation at 2000g for 3 min at 4oC and resuspended by Galbraith buffer with 5 µg/mL DAPI  prior to Florescence-activated nuclei sorting. Smart-seq3 single cell strategy