GSM8309109: AGY735_LOG_R2; Saccharomyces cerevisiae YPS1009; RNA-Seq
RNA was extracted using hot acid-phenol RNA extraction, with the following modification for quiescent samples: 500uM glass beads were added to each sample and vortexed every 10' for 1' during the hot acid-phenol incubation. rRNA depletion was performed using the Ribo-Zero (Yeast) rRNA Removal Kit (Illumina). Libraries were prepared with TruSeq Stranded Total RNA kit (illumina) and purified using a Axygen AxyPrep MAG PCR Clean-Up Kit (Axygen).