GSM7931894: EMseq_Aapp_Aza_rep3; Amoebidium appalachense; OTHER
Nitrogen liquid grinding followed by NEB Monarch Genomic DNA Purification Kit was used to extract the DNA of all samples. RNA was extracted using the Monarch Total RNA Miniprep Kit. Enzymatic Methyl-seq samples were performed as per manufacturer's instructions (https://www.neb.com/en-gb/products/e7120-nebnext-enzymatic-methyl-seq-kit) starting from 100 ng of DNA, after sonicating DNA to 300 bp fragments with a Covaris M220.