GSM7845004: U2-sh-1; Homo sapiens; RNA-Seq
mRNA high throughput sequencing was performed by NewCore Biotech. (Shanghai, China). Briefly, total RNA was extracted using Trizol Reagent (ThermoFisher, 15596026). The mRNAs were purified using a NEBNext Poly(A) mRNA Magnetic Isolation Module Kit (NEB, E7490) according to the user manual. mRNA libraries were constructed by using Illumina TrueSeq mRNA sample prepa_x0002_ration kit (Cat. No. RS-122-2101) according to the manufacturer's instructions. Library sequencing was performed on an Illumina NovaSeq 6000 instrument with 151 bp paired end reads.