S. aureus from human orapharyngeal sample
The genomic DNA of each sample was randomly sheared into short fragments of about 350 bp respectively. The obtained fragments were subjected to library construction using the NEBNext DNA Library Prep Kit, following the instructions strictly. Briefly, as followed by end repairing, dA-tailing, and further adapter ligation, the required fragments (in 300-500 bp size) were PCR enriched by P5 and indexed P7 oligos. After purification and subsequent quality check, the library is ready for sequencing.