GSM6732311: Amp_intestine-IP2; Mus musculus; RIP-Seq
Total RNA of RNA-seq samples were homogenized in 1 ml TRIzol (Accurate Biotechnology) and precipitaed by isopropyl alcohol. After total RNAs of each samples were extracted,the extracted total RNA was subjected to poly-A selection using Oligo d(T)25 Magnetic Beads (#S1419S, NEB). Purified poly-A RNA fragmentation to roughly 150-nt fragment size was performed according to the protocol with the Invitrogen RNA Fragmentation Reagents kit (Invitrogen). The purified poly-A RNA was fragmented and used for the construction of both m6A immunoprecipitation (IP) and untreated control (input) MeRIP-seq libraries. m6A IP was conducted using an EpiMark® N6-Methyladenosine Enrichment Kit (#E1610S, NEB) following the product manual. The resultant m6A-enriched and untreated poly-A RNA fragments were then used to construct strand-specific IP and input libraries for MeRIP-seq, respectively. All libraries were sequenced using Illumina NovaSeq 6000 by Berry Genomics. MeRIP-Seq