mitochondrial DNA-seq of Gymnogobius scrobiculatus (Takagi, 1957): Estuary silt fish
The sequencing libraries were prepared according to the manufacturers instructions of TruSeq PCR-free DNA High Throughput Library Prep Kit (Illumina). Briefly, 1ug of genomic DNA was shearedusing adaptive focused acoustic technology (Covaris) and the fragmented DNA is end-repaired tocreate 5-phosphorylated, blunt-ended dsDNA molecules. Following end-repair, DNA was sizeselected with bead-based method. These DNA fragments go through the addition of a single Abase, and ligation of the TruSeq DNA UD indexing adapters. The purified libraries were quantifiedusing qPCR according to the qPCR Quantification Protocol Guide (KAPA Library Quantification kitsfor Illumina Sequencing platforms) and qualified using the high-sensitivity DNA chip (AgilentTechnologies). And then the paired end (2150bp) sequencing was performed by the Macrogenusing the NovaSeq X (Illumina).