16S rRNA gene (V1-V9) of Loxodonta Africana: trachea
Amplification of full-length 16S rRNA amplicons (V1-V9) using 27F and 1492R primer set, PCR clean-up, library preparation with Nextera XT DNA flex Library Prep kit, library clean-up, library pooling with 10% PhiX spike-in, 50 pM pooled library sequenced in 2 x 150 bp reads on Illumina iSeq 100 instrument