GSM8279972: Model, scRNAseq; Mus musculus; RNA-Seq
Lung tissues were obtained from the control and asthma groups.The tissues were dissociated into single cells in dissociation solution.Single cell suspensions were loaded to 10× Chromium (10× Genomics, USA) to capture more than 3,000 single cells for the Single-cell 3′ Library using Gel Bead Kit V3 (1000075, 10× Genomics, USA) according to the manufacturer's instructions. The following cDNA amplification and library construction steps were performed according to the manufacturer's recommendation. Sequencing was performed on the NovaSeq6000 sequencer (Illumina, USA) with a minimum depth of 60,000~90,000 reads per cell and 150 bp (PE150) paired-end reads performed by LC-Bio Technology Co., Ltd (China). RNA: 3' transcriptome sequencing, cDNA fragmentation, end filling, adding A, connection connector, Index PCR.