scRNA-seq of Mus musculus: tumor immune infiltrates
EMT6 tumors were dissociated into single cells using a gentleMACS Octo Dissociator and a mouse Tumor Dissociation Kit (Miltenyi Biotec B.V. & Co. KG). CD45+ cells were isolated using a FACS Aria Fusion cell sorter (BD Biosciences) after presorting with anti-mouse CD45 MicroBeads (Miltenyi Biotec B.V. & Co. KG). The cDNA library was prepared from cells using a Chromium Controller and Chromium Next GEM Single Cell 3 GEM, Library & Gel Bead Kit v3.1 (10x Genomics, Pleasanton, CA). The cDNA library was sequenced using a NovaSeq 6000 sequencer (Illumina, Inc.).