GSM6595216: Prurigo nodularis, patient 2; Homo sapiens; RNA-Seq
Lymphocyte's concentrations were adjusted to load 2000 cells and enable the capture of 1000 cells per tissue sample. Cell suspensions were loaded onto separate chip inlet to perform cell encapsulation into droplets. Reverse transcription, cDNA generation and library preparation were achieve using the standard 10X Genomics single cell protocol (Chromium Single Cell 3  Reagent Kit V3.1.). The library was sequenced on the Illumina NovaSeq6000 platform following manufacturer s recommendations to obtain approximately 50 million reads per sample. Single cells were captured using the droplet-based microfluidic system Chromium (10x Genomics) according to the manufacturer's instructions. Lymphocyte's concentrations were adjusted to enable the capture of ~2 000 cells per tissue sample.  The standard protocol for the 10x single cell Chromium Single Cell 3' Reagent Kit V2 was followed and cells were loaded onto a separate chip inlet. The library was sequenced on the Illumina HiSeq 4000 platform following manufacturer's recommendations to obtain approximately 50 million reads per sample