RNA-Seq of drosophila melanogaster: whole body s40
For each fly sample (101 samples), the fly tissue was first dipped into liquid nitrogen and then crushed using a mortar and pestle. The Lysis Buffer B was added to the crushed sample and the mixture was transferred to a 2ml tube. The subsequent DNA isolation was done using Norgens Cell and Tissue DNA Isolation Kit (Cat.53100) according to the manufacturer's instructions. DNA QC was done using NanoDrop 2000 to assess its quality and quantity. For the 16S rRNA library prep step, we used Norgens 16s V3-V4 Library Preparation Kit for Illumina (Cat. 70410 and 70440) according to the manufacturer's instructions. Library QC was performed on a Bio-Rad CFX Touch 96 PCR machine using the Kapa Library Quantification kit (Cat. 07960140001) according to the manufacturer's instructions. The libraries were then pooled and sequenced on the Illumina Miseq platform, using the MiSeq  Reagent Nano Kit v3 (600 cycles).