GSM6386756: WT male rep1; Mus musculus; RNA-Seq
Spleens were removed from 4-6 weeks-old  Em-Myc transgenic mice, flash frozen on liquid nitrogen, and RNA was extracted using Nucleopsin RNA II (Macherey-Nagel). The quality of RNA was checked with Bioanalyzer Agilent and RNAs with a RIN > 6 retained for further analysis. RNA was depleted from ribosomal RNA, then converted into cDNA using a TruSeq Stranded Total Library preparation kit (Illumina).