Helcococcus ovis WGS
For Illumina short-read sequencing, genomic DNA (gDNA) was extracted using the DNeasy blood and tissue kit following the manufacturers instructions (Qiagen). Genomic DNA purity was measured using a NanoDrop 2000 spectrophotometer; final DNA concentration was confirmed with a Qubit 2.0 Fluorometer. Sample libraries were prepared using the Illumina DNA Prep kit and IDT 10bp UDI indices and sequenced on an Illumina NextSeq 2000, producing 2x151bp reads. Demultiplexing, quality control and adapter trimming were performed with bcl-convert (v3.9.3).