GSM7901218: hMSC R206H, BYL719, BMP6, 4; Homo sapiens; RNA-Seq
ReliaPrep RNA Miniprep Systems (Promega) mRNA was purified from tRNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTPs. Subsequent A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification and purification was performed to construct the directional library.