Halisarca dujardinii scRNA-seq experiment, biol. rep. 1, tech. rep. 2
To obtain single-cell suspension, sponge body was dissociated in Dounce homogenizer and passed through a syringe with an 18G needle diameter. Cell count and viability were assessed using trypan blue and automated Bio-Rad cell counter. Cells isolated from sponges were captured in droplet emulsions using a Chromium Single-Cell instrument (10x Genomics) and libraries were prepared using the 10x Genomics Chromium Controller and Chromium Single Cell 3 Kit v3. Single-cell libraries sequenced on an Illumina HiSeq 2500 with the following sequencing read recommendation Number of Cycles: 26 cycles Read1 for cell barcode and UMI, 8 cycles I7 index for sample index, and 98 cycles Read 2 for the transcript.