16S rRNA-seq of feces from mice
Microbial DNA was extracted from faecal samples using the E.Z.N.A. Soil DNA Kit. The V3-V4 hypervariable regions of the bacterial 16S rRNA gene were amplified with primers 338F and 806R by PCR. Purified amplicons were pooled in equimolar and paired-end sequenced (2  300) on an Illumina MiSeq platform.