WGS of Rhipicephalus turanicus: whole body
DNA was isolated from ticks stored in 70 % (v/w) ethanol. Dried ticks with incisions in their idiosoma were subjected to total tick genomic DNA (gDNA) isolation with the Monarch Genomic DNA Purification Kit (New England Biolabs, Australia). The gDNA isolated from four adult Rhipicephalus spp. ticks was used for genome skimming via next-generation sequencing (NGS) using a NEBNext DNA Library Prep Kit followed by NGS using 150 bp paired-end Illumina NovaSeq 6000 sequencing system (Novogene, Singapore).