whole genome sequence of Klebsiella pneumoniae DNA
Whole-genome metagenome sequencing libraries was prepared using KAPA DNA HyperPrep kit. The extracted genomic DNA was sheared using Covaris ultrasonicator and subjected to repairing the ends and tailing with dA-tail followedby ligation of indexed adapter sequences. Those fragments were cleaned with SPRI beads and enriched with limited cycles of PCR amplification. Prepared libraries were sequenced on Illumina HiSeqX sequencing platform.