DNA-Seq of mouse gut metagenome: inactivated recombinant CP8-EPSPS protein
Total DNA was extracted from stool samples (n=5, per group) using the Stool DNA Kit (TIANGEN, China). After sonication, the DNA sample was processed through various steps including end-polishing, A-tailing, ligation with adaptors, purification, and PCR amplification to construct the library. Once the library passed quality control, different libraries were pooled based on the desired concentration and target data volume of Illumina PE150 sequencing. The raw data underwent quality control and host filtering to obtain clean data (https://github.com/OpenGene/fastp) (Chen et al., 2018).