16S rDNA from lake water (GFC filter)
Water samples were filtered (1.5  2 L) through previously autoclaved glass fiber filters (GF/C) using low vacuum filtration. DNA was extracted using the the DNeasy Power Water Kit from GFC filters. Amplification of  16S rDNA V3-V4 region was done using, the two standard primers 341F-mod ILL (CCTAYGGGRBGCASCAG)  806R-ILL (GGACTACNNGGGTATCTAAT), (28 cycles).