RNA-seq of Z. indianus: naive hematocytes
RNA was isolated by RNeasy mini kit (Qiagen). polyA-RNAs were selected with NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) then strand-specific sequencing libraries were generated using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) with NEBNext Multiplex Oligos for Illumina (NEB)