Sediment 16S: River Sept Rep 3
DNA was extracted from 0.4 to 1.6 g wet weight of sediment using the PowerSoil DNA Isolation Kit. Library preparation, including amplification, and sequencing (Illumina MiSeq 2x300 bp paired-end V3 chemistry) were performed by the Integrated Microbiome Resource (IMR, Dalhousie University in Halifax, Nova Scotia, Canada) using standard protocols. Sequences in the V3-V4 region of the 16S rRNA gene were amplified using primers 341F (CCTACGGGNGGCWGCAG) and 805R (GACTACHVGGGTATCTAATCC) (Illumina / Klindworth 2012).