Y3.6
The Amplicon quality was visualized using gel electrophoresis. The PCR products were purified with Agencourt AMPure XP beads (Beckman Coulter Co., USA) and quantified using Qubit dsDNA assay kit. The concentrations were then adjusted for sequencing. Sequencing was performed on an Illumina NovaSeq6000 with two paired-end read cycles of 250 bases each. (Illumina Inc., San Diego, CA; OE Biotech Company; Shanghai, China)