RNA-seq of Rattus norvegicus
PolyA+ RNA was enriched by oligo(dT)25 Dynabeads (Invitrogen) from total RNA extracted as previously described (Yang et al., 2020). The dUTP-based strand-specific RNA-seq libraries for control and Ang II-induced RAEC cells were also constructed according to the method previously described (Yang et al., 2020). RNA-seq libraries were sequenced on Illumina HiSeq platform, and paired-end reads of 150 nucleotide (nt) lengths were obtained.