16s rRNA gene of sediment: Prairie wetland ponds
spectrophotometers. The V4 region of the 16S rRNA gene was amplified using a modified version of dual-index primer pair 515F/806R as described in Kozich et al., 2013. Sequencing adaptors and barcodes were bound to ~10 ng of amplicon DNA using Nextera DNA Prep (Illumina Inc.). Ninety libraries were sequenced using Illumina MiSeq V2, 2x250bp pair-end reads to ensure coverage of the whole region of V4 (254 bp) with 246 bp overlap.