45 h Sample of Dioscorea opposite cv. 'Tiegun'
Calluses of A. tenuis and P. asturianum infected for 45 h and the CK were collected to extract the total RNA according to the FastPure Universal Plant Total RNA Isolation Kit (Vazyme, Nanjing, China) instructions. Subsequently, RNA quality was tested and the qualified samples were sent to the Majorbio BPT Co., Ltd. (Shanghai, China) for transcriptome sequencing analysis through Illumina MiSeq platform. Defined log2 (Alternaria or Penicillium)/CK ratios 1 or -1 as the differentially expressed genes (DEGs).