RAD-sequencing of rare asexual Artemia males, their mothers and sisters.
protocol from Johnson et al. 2008 'Rapid SNP Discovery and Genetic Mapping Using Sequenced RAD Markers'. DNA was digested by PstI. P1-ligated fragments were sonicated, then size-selected by gel migration. P1 and P2-ligated fragments were amplified by 12 PCR cycles.