Oxford Nanopore Technology genomic sequences of a pool of 15 Braula coeca flies
Genomic DNA was extracted from 15 unsexed individuals conserved in alcohol, using the Nucleobond AXG20 kit and buffer set IV from Macherey-Nagel (ref. 740544 and 740604, https://www.mn-net.com, Dren, Germany). Size selection was conducted on the DNA using the SRE XS from Circulomics (https://www.circulomics.com/, Baltimore, Maryland, USA). The SQK-LSK110 kit from ONT (https://nanoporetech.com/)72 was then used to prepare the samples for nanopore sequencing following manufacturers protocol. The library was loaded and sequenced on a R9.4.1 flow cell (ref. FLO-Min106) for sequencing.