Identification and evaluation of P. falciparum mature gametocytes genotype markers using amplicon deep sequencing.
DNA and RNA was extracted from blood samples preserved in RNA protect cell reagent using RNeasy Plus 96 kit (RNA) and QIAamp DNA Blood Kit (DNA) and amplicons generated using the KAPA HiFi HotStart ReadyMix PCR kit; Amplicon sequencing was performed on Illumina MiSeq platform with generation of 300 bp paired-end reads.