Gill Transcriptome of Cyprinus carpio infected with Klebsiella pneumoniae
PE Illumina libraries were loaded onto NextSeq500 for cluster generation and sequencing. Paired-End sequencing allows the template fragments to be sequenced in both the forward and reverse directions on NextSeq500. The kit reagents were used in binding of samples to complementary adapter oligos on paired-end flow cell. The adapters were designed to allow selective cleavage of the forward strands after re-synthesis of the reverse strand during sequencing. The copied reverse strand was used to sequence from the opposite end of the fragment.