Negative plant soil feedback influences a dominant seeded species in grassland restoration
The ITS2 region was amplified to characterize general fungi communities (PCR1) using forward fungal primers flITS7 and flITS7o and the reverse primer ITS4. Following index linker addition by PCR (PCR2), amplicons were purified using AMPure XP beads, and pooled based on band strength to equalize concentrations prior to sequencing.