Plasmid samples from Escherichia coli
The library for NGS sequencing was prepared using Swift 2S Turbo DNA Library Kits (Swift Biosciences, Ann Arbor, Michigan, United States). Briefly, 100 ng genomic DNA was fragmented, end prepped and adapter-ligated. Magnetic beads size selection was performed to select 250-300 bp insert size fragments followed by the library amplification according to the manufacturer's instructions. The quality of the library was checked on 4200 TapeSation System using D1000 Screen Tape (Agilent Technologies, Palo Alto, California, USA) and the quantity was measured on Qubit 3.0. (Thermo Scientific, Waltham, Massachusetts, USA). Illumina sequencing was performed on NovaSeq 6000 instrument (Illumina, San Diego, California, USA) with 2x151 run configuration.