MiSeq of mus musculus stool:High fat diet fed Villin-Cre- mice day 105
Feces were collected from mice after (day 105) HFD treatment. Fecal DNA was isolated using a QiAMP stool DNA extraction kit (QIAGEN). Analysis utilizing PCR amplification of the V4 region of 16S rRNA followed by sequencing on an Illumina MiSeq was performed by MR DNA (Shallowater, Tx, USA).