amplicon sequencing: 24 day Hu lamb rumen epithelium of STA group relicate 2
Total DNA was extracted from samples by the cetyltrimethylammonium bromide method (Brookman and Nicholson, 2005). A Qubit 2.0 fluorometer (Life Technologies, Carlsbad, CA, USA) with a Qubit double-stranded DNA (dsDNA) HS assay kit (Life Technologies, Carlsbad, CA, USA) were used for measuring the concentrations of the DNA samples. The primer set 515F/806R targeted the V4 hypervariable region of the 16S rRNA gene was used to generate amplicon library from the DNA samples (Caporaso et al., 2011).