RNA-Seq
The BGISEQ-500 sequencing platform (BGI, Qingdao, China) was employed for transcriptome analysis, resulting in the generation of seven paired-end fragment libraries (2  100 bp), collectively producing 140 Gb of raw sequencing data. To ensure data quality, the raw data underwent quality assessment using FastQC v0.11.8.40 Adaptor sequences and low-quality reads were subsequently removed. The resulting clean reads were subjected to de novo assembly using Trinity v2.5.141 with default parameters, yielding a total of 332,785 unigenes.