LPS/Nig and 2-BP treatment third repeat
RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina usingmanufacturers instructions (NEB, Ipswich, MA, USA). Briefly, mRNAs were initially enriched with Oligod(T) beads.Enriched mRNAs were fragmented for 15 minutes at 94 C. First strand and second strand cDNA weresubsequently synthesized. cDNA fragments were end repaired and adenylated at 3ends, and universal adapterswere ligated to cDNA fragments, followed by index addition and library enrichment by PCR with limited cycles.The sequencing library was validated on the Agilent TapeStation (Agilent Technologies, Palo Alto, CA, USA), andquantified by using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA) as well as by quantitative PCR (KAPABiosystems, Wilmington, MA, USA).