Transcriptome data of stipe of Flammulina filiformis
(1) Eukaryotic mRNA was enriched by magnetic beads with Oligo (dT);(2) The Fragmentation Buffer was added to randomly interrupt the mRNA.(3) Using mRNA as template, the first cDNA strand was synthesized with random hexamers, and then the second cDNA strand was synthesized by adding buffer, dNTPs, RNase H and DNA polymerase I. AMPure XP beads were used to purify cDNA.(4) The purified double-stranded cDNA was subjected to end repair, A-tail addition, sequencing adaptor, and AMPure XP beads for fragment size selection.(5) cDNA library was obtained by PCR enrichment