KOD DNAP 2pt library pre-selection
The 2-point saturation mutagenesis library targeting the catalytic D404 and its vicinal L403 residue was generated using saturation mutagenis primers KOD_Sat_403-404_F1 (CATAGTGTACNNKNNKTTTAGATCCCTGTACC) and KOD_Sat_403-404_R1 (TTCTCCCACAACCCTCTC) on an exonuclease deficient KOD DNAP (D141A/E143A) background (KOD exo-) through inverse PCR (iPCR).