RNA-seq of Kelletia kelletii: embryo
Briefly, RNA was extracted using Trizol and RNA phase separation. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dTTP for non-directional library preparation. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. Libraries were prepared using end repair, A-tailing, adapter ligation, size selection, amplification, and purification.