mRNA sequencing of B16 LD day 6 samples siMCUb
The libraries were prepared from 500 ng of intact RNA using NEBNext Ultra II RNA Library Prep Kitfor Illumina (NEB, Cat. No. E7770L) according to the manufacturers protocol and the library QC wasdone using Agilent D1000 screentape (Agilent, Cat No. 5067-5582) and Qubit DNA HS (Thermo,Q33230).