WGS of Candida albicans: SC5314 CRISPR-competent sir2/sir2::SIR2 alpha/-
-80C frozen stock were struck onto solid YPD medium and grown at 30C for two days, after which the strains were processed for sequencing by SeqCoast Genomics (Portsmouth, NH, USA). Briefly, DNA from each strain was extracted using the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit and prepared for whole genome sequencing using the Illumina DNA Prep tagmentation kit and unique dual indexes. Sequencing was performed on the Illumina NextSeq2000 platform to produce 2x150bp paired reads. Reads were demultiplexed, trimmed, and assessed using FastQC.