shallow shotgun sequencing of the human gut microbiome
According to the manufacturers instructions, DNA library preparation was performed using the Nextera XT DNA sample preparation kit (Illumina, San Diego, CA, USA). First, 1 ng input DNA from each sample was used for the library preparation, which underwent fragmentation, adapter ligation and amplification. Then, Illumina libraries were pooled equimolarly, denatured and diluted to a concentration of 1.5 pM.