ONT whole genome sequencing of Hordeum vulgare subsp. Vulgare, line Morex untreated (FASTQ): accession Morex
The Nanopore protocol was used to extract DNA from the sodium-azide treated Morex samples. The QIAGEN Genomic-tip 500/G kit and Carlson buffer were used in the DNA extraction process. Following DNA extraction, read sizes were chosen to concentrate on particular regions of interest. The Rapid Kit SQK-RAD004 was used for DNA sequencing. The MinION Mk1C (MIN-101C) was used to carry out the actual sequencing. The FLO-MIN106D flow cell, which is a specific model compatible with the MinION Mk1C sequencer, was used during the sequencing process.