RADseq of Asterias: from tissue
Library preparation and sequencing for 190 samples was completed by Floragenex (Portland, OR) using restriction site-associated DNA sequencing (RADseq) with established protocols (Lozier 2014). These samples were processed into a single RAD library using the enzyme SbfI-HF (NEB). After sonication to shear the DNA, initial size selection was ~300-500bp and final size selection ~400-600bp (including adapters). Size selection was performed using an agarose gel and the Qiagen MinElute Gel Extraction Kit, and the completed library was sequenced on an Illumina HiSeq 4000 sequencer.