RNA-Seq of Drosophila melanogaster CS; male abdomen thorax and heads on low, high calorie dietand diet shifting
Total RNA was isolated from the heads, thoraces, and abdomens of 3 biological replicates with more than 50 flies in each replicate using Trizol (Invitrogen, cat# 15596026). One mg of each RNA sample was used to prepare RNA-seq libraries with the Illumina Tru-Seq Stranded mRNA Library Preparation kit (cat # 20020594) with the IDT for Illumina TruSeq RNA UD Indexes set (cat # 20040871). The libraries were pooled and sequenced on one lane of a Illumina NovaSeq 6000 S4 flow.