CifA(01614)
polyA tail mRNA was enriched by OligodT beadsrRNA removal: DNA probe was used to hybridize rRNA, and RNaseH selectively digested DNA/RNA  hybridization chain, and then DNaseI was used to digest the DNA probe, and the required RNA was obtained after purification.The obtained RNA was segmented with the interrupt buffer, and the random N6 primers were used for reverse transcription, and then the cDNA two strands were synthesized to form double-stranded DNA.The synthetic double-stranded DNA ends are flattened and phosphorylated at the 5 'end, with the 3' end forming a sticky end protruding anAand connect a bubbling joint with a 3' end projecting a "T". The connection products were amplified by PCR using specific primers.The PCR products were thermally denatured into single strand, and then the single strand circular DNA library was obtained by using a segment bridge primer.