feces of participant
The fecal gut flora and serum lipid metabolism studies were performed by Gene Denovo Biotechnology Co., Ltd (Guangzhou, China). Gut flora DNA was extracted from the fresh feces of participants using HiPure Stool DNA Kits (Magen, Guangzhou, China). The V3-V4 hypervariable regions of the 16S rRNA gene were amplified with the primers 341F (CCTAYGGGRBGCASCAG) and 806R (GGACTACNNGGGTATCTAAT). Equimolar purified amplicons were pooled and sequenced on the Illumina NovaSeq 6,000 PE250 platform (Genedenovo Biotechnology Co., Ltd, Guangzhou, China).