RNA-seq of Tfas_A, timepoint D+9
Six plants per species were placed in a climate chamber and sampled every four hours in a 24-hour cycle. Total RNA was extracted using the QIAGEN RNeasy Mini Kit in an RNAse free laboratory. Samples were digested using the kits RLT buffer with 1 % Beta-mercaptethanol. Elution was done in two steps. The purity and concentration of the extractions was measured using Nanodrop, and RIN and fragmentation profiles were obtained with a Fragment Analyzer system. RNA libraries were prepared by the Vienna Biocenter Core Facilities (VBCF) using a NEBNext stranded mRNA kit before sequencing 150-bp paired-end reads on one lane of Illumina NovaSeq S4.