MDA-MB-231 resistant to paclitaxel siBRG1 RNA-Seq
RNA-Seq: 250 000 cells were transfected with siCTRL or siBRG1 using Silencer Select siRNA and Lipofectamine RNAiMAX (Thermofisher Scientific). After 72 h cells were harvested in TRI Reagent (Thermofisher Scientific), mRNA was isolated using NEBNext Poly(A) mRNA Magnetic Isolation Module and RNAseq library was created with NEBNext Ultra II RNA Library Prep Kit for Illumina (#E7770). Sample indexing was prepared using NEBNext Multiplex Oligos for Illumina (Index Primers Set 3) (#E7710).