Red Spruce (Picea rubens) Chloroplast Genome Sequences
The DNA was briefly fragmented into small fragments using nebulization following standard blunt-ending and add A was performed. The adapters were ligated to the ends of the DNA fragments and a purification step was performed to remove the non-ligated adapters. Further, size selection in the range of 200-250bp of the adapter ligated library was performed on low range agarose gel fol-lowing PCR amplification to selectively enrich the DNA fragments with adapters on both the ends. The quantity of the prepared library was estimated using Qubit 2.0 Flu-orometer (Invitrogen, Carlsbad, CA, USA) and the quality tested by Agilent 2100 Bio-analyzer High Sensitivity DNA assay (Agilent technologies, Santa Clara, CA, USA). Library was loaded onto Illumina c-Bot Cluster Station following the manufacturers protocol and sequenced with single-end 44 bp reads on Illumina Genome Analyzer II (GAII, Illumina Inc.).