PacBio DNA-Seq of Rubus idaeus: young leaves
Genomic DNA was extracted from young leaves using the NucleoSpin Plant II Midi kit (MACHEREY-NAGEL, Dren, Germany). A long read (5-20kb) PacBio genomic library was constructed using SMRTBell Template Prep Kit and sequenced on two SMRT cells on the PacBio Sequel System.