Deep sequencing of AGT, MGMT, and TP53 genes from human: astrocytic tissue from adult male patient
DNA was extracted from blood with Wizard Genomic DNA Purification Kit. A customized Ion Ampliseq panel directed at AGT, MGMT and TP53 exons, untranslated regions and adjacent intronic bases was designed. Libraries were constructed using Ion AmpliSeq Library Kit 2.0. Sequencing was done with a 316 chip. Ion View OT2 Iit was used for sequencing.