RNA-Seq of Fokienia hodginsii: mature cones
Before building the library, the RNA samples were measured for RNA concentration and fragment length, followed by oligo(dT) magnetic beads to enrich the mRNA, and then the frogmentation buffer was used to break the mRNA into short fragments. A single strand of cDNA is then retrotransformed and then synthesized into a double strand of DNA, which is purified and pcrographed to build a cDNA library