16S of marsh soil: salinity addition experiment
Marsh soil was collected with a sterilized soil corer at 10cm depth. Soil was homogenized in a ziplock and kept on wet ice until returning to the lab. That day, PMAxx was added to sample to remove relic DNA from downstream amplification. After the PMAxx protocol, samples were frozen at -20C until DNA extraction. DNA extraction was performed with Qiagen power soil pro, where the input material was 960mL of soil from the PMAxx protocol (soil suspended in buffer). DNA extractions were standardied and amplified with dual-step PCR before purification with AMPure. Samples were pooled and sequenced on Illumina miseq 300bp PE