fungal community of Triticum aestivum: roots
A nested PCR approach was used. For the first PCR, the primers SSUmAF and LSUmAr were used. For the second PCR, LSUD2mod and LSUmBR were used.  Third PCR to barcode the samples and MiSeq sequencing was performed by Next Generation Sequencing Facility at Vienna BioCenter Core Facilities.