RNA-seq of Aedes aegypti midguts
Female Aedes aegypti mosquitoes were artifficially fed on commercial human blood (BF). Intact midguts (15 per bioological replicate) were collected at 44h after blood feeding and placed directly into Trizol reagent. RNA extraction was performed according to manufacturer`s specifications and analyzed with an Agilent 2100 Bioanalyzer (Agilent, USA). The library was constructed using the NEBNextUltraTM II (Directional) RNA with polyA selection library prep kit and sequencing was performed in an Illumina Novaseq 6000 DNA sequencer.