Single cell RNAseq of mock-infected NHBE cells
NHBE cells were infected with Cal/04/09 at an MOI of 0.5. 10 hours post infection cells were washed and trypsinized to generate single cell suspensions. Single cell suspensions were filtered and counted and 10,000 cells per condition were used to generate Gel Bead-in Emulsions (GEMs). The 10X Chromium 3' GEX Capture kit and the 10X Chromium controller were used to generate GEMs and barcoding per manufacturers protocol (10X Genomics, Pleasanton, CA, USA). GEMs generated were used for cDNA synthesis and library preparation using the Chromium Single Cell 3' Library Kit and run on a NovaSeq S2. 2x100 PE Run.