16s RNA
IIumina-based Next-generation sequencing was used for this analysis. 16S rRNA  (V3-V4) region of the bacterial cell was amplified using the primer sequences for the forward strand: 5 AGAGTTTGATGMTGGCTCAG3 (F) and 5 TTACCGCGGCMGCSGGCAC3'(R) for the reverse strand. The polymerized chain reaction (PCR) was completed with the first denaturation step at 95 C (15 seconds), annealing at 60 C (15 seconds ), elongation at 72 (2 minutes), and the final extension step for 10 minutes and hold at 4 C for 25 cycles. 40 ng of the DNA was used for amplification along with 10 pM (picoMolar) of each primer.