RNA-Seq of visceral adipose tissue: male rats
The BGSEQ-500 (BGI-Shenzhen, China) platform was used for gene sequencing to obtain raw reads. The clean reads were mapped to the rat genome (Rattus_norvegicus, UCSC, rn6) using HISAT2 (Hierarchical Indexing for Spliced Alignment of Transcripts), and Bowtie2 (version 2.2.6) was utilized to align the clean reads for the reference coding gene set. The expression was then calculated as fragments per kilobase million (FPKM).