Whole genome sequence of phenotypically optimized genomically recoded Escherichia coli: C321.ilvG
Genomic dsDNA was purified from 3 ml of bacterial culture grown to saturation at 34 C in LB medium using the Qiagen DNeasy dsDNA extraction kit (catalog no. 69504) following manufacturer protocols. DNA samples were submitted to the Yale Center for Genome Analysis for fragmentation and Illumina library preparation. Fragmented DNA samples were subjected to 10 cycles of PCR using NNNNNN hexamer primers to increase DNA concentrations prior to adaptor ligation. Samples were sequenced using the Illumina NovaSeq platform.