A14_CKDL210003263-1a_HFVYFBBXX_L5
Total RNA was extracted using Quick-RNA MagBead kit (Zymo Research) from cryopreserved PBMC. RNA concentrations were then quantified using a Qubit Fluorometer (Invitrogen), and RNA integrity was assessed using the Agilent TapeStation (Agilent). Indexed libraries were constructed using the SMARTer Stranded Total RNA-Seq Kit v3 (Takara Bio) following the manufacturer's instruction. The quantity and quality of the libraries were assessed by Qubit and Agilent 2100 Bioanalyzer, respectively. Libraries' molar concentrations were validated by qPCR for library pooling.