Sanger sequencing of S. cerevisiae carrying either a test construct with the LoxPsym variant array, after induction of recombination
Amplicons spanning the recombined LoxPsym array after induction of recombination. Amplicons were generated via PCR from 3 randomly selected colonies with correct fragment size or clones with incorrect size,  for each LoxPsym array layout. Amplicons were sent for Sanger sequencing at the Eurofins Genomics.