Rhynchospio RNAseq
RNA was extracted and purified from RNAlater-preserved tissue using the Omega Bio-Tek Mollusc RNA kit with on-column DNAse digestion. One microgram of total RNA was sent to Macrogen, Cambridge, Massachusetts for Illumina TruSeq RNA v2 library preparation (polyA enrichment) and sequencing on the Illumina HiSeq 2500 system using the HiSeq SBS V4 chemistry with 100bp paired-end sequencing.