RNA-seq of cup1delta upf1delta Saccharomyces cerevisiae: Rep 1
Cell walls were disrupted by incubation with Zymolyase (50 U/mL, Zymo Research). Total RNA was then isolated using TRIzol reagent (Thermo Fisher Scientific) according to manufacturers instructions. Samples were cleaned using a 50 g Monarch RNA Cleanup Kit (New England Biolabs) and treated with TURBO DNase (Thermo Fisher Scientific) to remove residual contaminating DNA. RNA libraries for RNA-seq were prepred using the TruSeq2 Library Prep Kit (Illumina) by the University of Wisconsin-Madison Biotechnology Center Gene Expression Center using the manufacturer's instructions.