miRNA-Seq of Aedes albopictus: avid-biting adult female specimens
Head tissue was disrupted in a Spex Genogrinder 2100 (2 x 1500 RPM for 2 minutes). RNA was extracted using a Zymo Direct-Zol kit (Zymo Research) and  Integrity was assessed with an RNA chip (Bioanalyzer 2100). miRNA was isolated using Perkin Elmers NextFlex small RNA-seq kit v3 (NOVA-5132-05) . Samples were utilized for single-end library construction and libraries were sequenced on a single lane.