RNA-Seq of Homo sapiens: pediatric skin tissue
approximately 30 mg of tissue from RNAlater were thawed and lysed in with 300 ul RLT (Qiagen, RNEasy Fibrous Tissue Mini Kit cat# 74704), 0.05% DX Reagen (Qiagen, cat#19088) and 3.0 mm stainless steel balls. The Nanodrop (ThermoFisher), Qubit 3 (LifeTechnologies) and 4200 TapeStation (Agilent Technologies) were used for RNA quantity and quality verification. Ribosomoal RNA were removed with RiboMinus Eukaryote System v2 (Life Techonology, cat# A15026/cat#15027). The Ion Total RNA-seq Kit v2 protocol (ThermoFisher publication no MAN0010654, revision C.0), was used for library preparation.