PSU067
Genomic DNA of B. pseudomallei isolates extracted using Wizard Genomic DNA Purification Kit (Promega Inc., Wisconsin, USA) were used in both techniques. The latter, the DNA samples were sequenced using Illumina sequencing methods. Briefly, Illumina sequencing libraries were prepared using the tagmentation-based and PCR-based Illumina DNA Prep kit and custom IDT 10 bp unique dual indices (UDI) with a target insert size of 320 bp. No additional DNA fragmentation or size selection steps were performed. Illumina sequencing was performed on an Illumina NovaSeq 6000 sequencer in multiplexed shared-flow-cell runs, producing 2x151 paired-end reads.