Chicken gut content group C 7
Samples were thawed at controlled temperatures before DNA isolation with the QIAamp PowerFecal Pro DNA Kit (QIAGEN). The isolated DNA underwent PCR amplification of 16S rRNA gene regions using specific primers from the 16S Barcoding Kit (Oxford Nanopore). Post-PCR, samples were prepped for sequencing following the Oxford Nanopore library preparation guidelines and sequenced on the MinION device. Sequencing output was processed via MiniKnow software for quality checks and base calling, with subsequent taxonomic classification done using the Epi2Me platform (Fastq 16s workflow).