DNA of Mugil cephalus: mature female tail
After the DNA sample is tested to be qualified, it is randomly interrupted by covaris ultrasonic crusher, and then the whole library is prepared through end repair, adding a tail, adding sequencing linker, purification, PCR amplification and other steps. After the library is constructed, qubit 2.0 is used for initial quantification, the library is diluted, and then the inserted fragment of the library is detected with Agilent 2100. After the size of the inserted fragment meets the expectation, the effective concentration of the library is accurately quantified using Q-PCR method to ensure the quality of the library