RNA-seq of cocultivation of HUVEC and human osteoblasts: osteoblasts in standart cultivation biorep 10
Total RNA was isolated by the standard phenol-chloroform extraction. Poly(A)-fraction was used for cDNA-libraries preparation with the NEBNext RNA Library Prep Kit. The libraries were sequenced by either Illumina HiSeq 2500 in PE-100 bp mode.