RNA-Seq of agdp3-1 seedlings_Replicate 3
mRNA was enriched and isolated from the total RNA sample using Oligo (dT) magnetic beads, then add fragmentation buffer to generate short RNA fragments. Synthesize 1st strand cDNA using random hexamers as primer and the fragmented RNA as template, then add reaction buffer, dNTPs, DNA Polymerase I and RNase H to synthesize 2nd cDNA strand. Purify the double-strand cDNA using AMPure XP beads, then add 3' A tail and ligate the sequencing adaptor to the cDNA. Finally the ligated cDNA was PCR amplified to get the sequencing library.