Whole-exome seq_adult male lung
Genomic DNA purity and concentration were assessed through PicoGreen dsDNA assay (Invitrogen) and agarose gel electrophoresis. The genomic fragment library was constructed using the SureSelelct v5 kit (Agilent Technologies) and subsequently subjected to sequencing on the Illumina HiSeq 2500 platform, employing targeted sequencing to capture 171 cancer-related genes. The resulting reads were aligned to the human genome reference (hg19) using Burrows-Wheeler Alignment. Followed by analysis with the Genome Analysis Toolkit. Somatic mutations were identified using MuTech2 and annotated using Oncotator.