RNA-seq of homo sapiens : somatotroph adenoma
To construct cDNA libraries, 0.5 g RNA and Illumina TruSeq Stranded Total RNA Library Prep Gold Kit (Illumina, Inc., San Diego, CA, USA, #20020599) were used, and the results were quantified by quantitative polymerase chain reaction using the TapeStation D1000 ScreenTape (Agilent Technologies, # 5067-5582). Indexed libraries were then subjected to Illumina NovaSeq (Illumina, Inc., San Diego, CA, USA) and paired-end (2101 bp) sequencing was performed by Macrogen (Seoul, Korea). The output data were demultiplexed using bcl2fastq v2.27 generating fastqc files.