WGA of Sus scrofa gut metagenome: Diarrhoea of Zinc Oxide pigs 7 days post weaning of farm A
Samples were immediately snap-frozen on dry-ice upon collection and transported to the research facilities where they were stored at -80 C until processing. The DNA from the faecal samples was extracted using the QIAamp PowerFecal Pro DNA Kit (Qiagen, Crawley, West Sussex, UK) following the manufacturers instructions, using 200  50 mg of faecal content. A Qubit fluorimeter (Qubit 3, Invitrogen) was used to determine the total DNA concentration. Paired-end sequencing libraries were prepared from the extracted DNA using the Illumina Nextera XT Library Preparation Kit (Illumina Inc., San Diego, CA) followed by sequencing on the Illumina NextSeq 500 platform using high-output chemistry (2  150 bp) according to the manufacturers instructions. Library size from each sample was assessed on an Agilent Technology 21000 Bioanalyzer using a High Sensitivity DNA chip.