16S rRNA gene amplicon sequecing of Arabidopsis Col-0 roots  inocaulted with SynCom II
DNA was extracted using DNeasy Powersoil Kit (Qiagen) according to manufacturer instructions. Primary PCRs performed using primers 341F and 781R with a mixture of two peptide nucleic acids (PNA) to block plant mitochondrial and plastid sequences. Secondary PCRs were performed using Nextera XT forward and reverse primers to index samples. Samples were sequenced on an Illumina MiSeq machine using Version 3 chemistry, 600-cycle flow cell for paired-end reads at 300 basepairs (bp).