16S rDNA amplicon seq of bacteria: surrounding soil
Total metagenomic DNA was extracted by using the PowerSoil DNA Isolation Kit, and examined by using 1% agarose gel electrophoresis before the DNA samples were stored at 20C in a freezer. Then a full-length 16S rRNA gene sequencing was used to create the sequencing library of 16S rRNA gene (16S rDNA).