scRNA-seq of mus musculus: adult skin MRSA
Single cell RNA-Seq libraries was prepared with Chromium Next GEM Single Cell 3 Reagent Kits v3.1 on the Chromium Controller (10Genomics). The cell suspension was loaded onto the Chromium Next GEM Chip G and ran the Chromium Controller to generate single-cell gel beads in the emulsion according to the manufacturers recommendation. Captured cells were lysed and the released RNA was barcoded through reverse transcription in individual GEMs. Barcoded, full-length cDNA was generated, and libraries were constructed. The quality of libraries was assessed by Qubit 4.0 and the Agilent 2100. Sequencing was performed on the Illumina NovaSeq 6000 with a sequencing depth of at least 50,000 reads per cell and 150 bp (PE150) paired-end reads (performed by Biomarker Technologies Corporation, Beijing, China).