RNA sequencing of UTEX 3222, BG11 Media, Replicate 4
Ribosomal RNA depletion and strand-specific RNA-sequencing was carried out by Azenta Biosciences. Samples for this purpose were grown in 50mL unbaffled flasks, at 37C and 200E light, shaken at 220 RPM in a growth chamber maintaining 0.5% CO2 for 16 hours. Four such replicate 10mL cultures were prepared in either BG11 medium or AD7 medium. Sequencing resulted in >40M 2x151bp read pairs for each sample.