Metabarcoding of Insect traps from grains - PCR control
Metabarcoding libraries were prepared using a 2 step PCR approach. First, fwhf2-fwhR2n primers were used to amplify the COI loci for 30 cycles. This was followed by 7 cycles of real-time PCR to attach 8bp unique-dual indices and Illumina sequencing adapters. Two replicates were used for this sample. Illumina bcl2fastq was used to demultiplex the resulting sequence reads.