Whole Genome Sequencing of Albugo candida isolate AC-BPM infecting Brassica juncea
DNA extraction was done by DNAeasy plant mini kit( Qiagen #69106). Extracted DNA quantity is confirmed using nanodrops 1000. 79.8(ng/microliter) DNA was obtained from the DNA extraction step. The library preparation was carried out by using KAPA HyperPlus Kit. (Roche #07962428001) Illumina paired end library was constructed based on the standard procedure as per the kit manufacturers instructions. The Extracted DNA was fragmented with enzymatic fragmentation followed by adapter ligation. The produced library had an average insert size of 260 bp based on tapestation run. The library was sequenced in Illumina Novaseq 6000 sequencer with 150 cycles so that the sequenced read length is 150 bp