antonovka_172670B_raw
High-molecular-weight genomic DNA extraction and Single Molecule Real Time (SMRT) Pacific BioSciences (PacBio) sequencing was performed at the Genotyping Center of the University of Delaware (DL, USA) using a DNeasy Plant Mini Kit (Qiagen) according to the manufacturers protocol. The DNA was fragmented into 15 kilobase pair (Kbp) fragments, and the HiFi library was prepared using SMRTbell Express Template Prep Kit 2.0 and the DNA/Polymerase Binding Kit 2.0 (Pacific Biosciences) according to the manufacturers protocol. The library was filtered for fragments of >10 Kbp in size by using Sage Blue Pippin (Sage Sciences) to remove smaller fragments and adapter dimers. PacBio Sequel II was used in CCS/HiFi mode with two SMRT cells with 2 hours of pre-extension and 30-hour movie times to sequence the library. Finally, read length distribution and quality of all HiFi reads was assessed using Pauvre v0.1923 (Schultz et al., 2018).