RNA-Seq of Homo sapiens: Human immature dental pulp stem cell
Cells were cultivated in DMEM/Ham's F12 medium, supplemented with 15% of Hyclone serum, 1% penicillin/streptomycin, 2 nM L-glutamine and 2 nM nonessencial aminoacids (all from Gibco, Carlsbad, USA) untill the fifth passage. RNA was isolated using TRIZol Reagent (Invitrogen, Carlsbad, USA) and the RNA quality was verified using the BioAnalyzer 1000. RNA subjeted to RNA-Seq showed RIN > 7