Nanopore sequencing of PCR-amplified human transcripts
Total mRNA was extracted and retro-transcribed using primers specific for the ETV4 transcripts. The PCR products from multiple cell lines were then used to prepare barcoded libraries for Ocford Nanopore. The libraries were pooled and run on Flongle flow cells (v.9.4.1 pore), using the MK1B sequencer on the MinKNOW software (v.19.2.2) (Oxford Nanopore Technologies). Base calling was performed using GUPPY (v.3.0.3)