Negative plant soil feedback influences a dominant seeded species in grassland restoration
The ITS1 region was amplified to characterize oomycete communities (PCR1) using the forward primer OOM18S (Legeay et al., 2019) and the reverse primer ITS7ae, a degenerate form of ITS7 (Taheri et al., 2017). Following index linker addition by PCR (PCR2), amplicons were purified using AMPure XP beads, and pooled based on band strength to equalize concentrations prior to sequencing.