DNA-Seq of nectopsyche paramo
We collected a female individual of Nectopsyche paramo (ER08) in Ecuador, Pichincha, Parque Nacional Cotopaxi, Ro Pita (0.61957S, 78.38332W) at 3,875 m elevation.We extracted high molecular weight DNA from the single individual using the Qiagen Genomic-tip extraction kit and prepared a low-input DNA sequencing library following the instructions of the SMRTbell Express Template Prep Kit 2.0 (PacBio). The library was sent to Radboud UMC, Nijmegen, The Netherlands. One SMRT cell sequencing run was performed on the Sequel System II in CCS (circular consensus sequencing) mode using 30-h movie time.HiFi reads were generated from the subreads using PacBio SMRTlink software pbccs/4.2.0-1 (https://github.com/PacificBiosciences/pbbioconda) with default settings.