MiSeq of mus musculus stool: Normal diet fed Villin-Cre- mice day 0
Feces were collected from mice before (day 0)  HFD treatment. Fecal DNA was isolated using a QiAMP stool DNA extraction kit (QIAGEN). Analysis utilizing PCR amplification of the V4 region of 16S rRNA followed by sequencing on an Illumina MiSeq was performed by MR DNA (Shallowater, Tx, USA).