RNA-seq of mono-culture using strain Clostridium sp. HGF2 at a late time
Ribosomal RNA depletion was performed with QIAseq FastSelect5s/16s/23s Kit (Qiagen). Samples were randomly primed and fragmented based on manufacturers recommendation. The first strand was synthesized with the Protoscript II Reverse Transcriptase with a longer extension period, approximately 40 minutes at 42C. All remaining steps for library construction were used according to the NEBNext UltraTM II Directional RNA Library Prep Kit for Illumina (New England BioLabs Inc., Massachusetts, USA).