Metatranscriptomics of Miami-Dade County wastewater
Sequencing libraries were generated using the NEBNext rRNA Depletion Kit and (Human/Mouse/Rat) NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) for Illumina. The KAPA Library Quantification Kit (Roche) was employed to quantify the resulting libraries, which were next evaluated and pooled at equimolar concentrations. Sequencing was carried out on the NovaSeq 6000 platform (Illumina) with an S4 flow cell, using a read length of 2 x 151 bp in paired-end configuration.