RNA-Seq of populus: wi1dtypeline1
(1) Enrichment of eukaryotic mRNA with magnetic beads with Oligo (dT);    (2) mRNA was randomly interrupted by Fragmentation Buffer.    (3) mRNA was used as template to synthesize the first cDNA strand with six-base random hexamers, and then the second cDNA strand was synthesized by adding buffer, dNTPs, RNase H and DNA polymerase I. cDNA was purified by AMPure XP beads.    (4) The purified double-stranded cDNA was then end-repaired, A-tail was added and sequencing joints were connected, and AMPure XP beads were used for fragment size selection;    (5) cDNA library was obtained by PCR enrichment