Endophytic diversity associated with leaves of Anthemis cotula
Metagenomic DNA samples  were  amplified using KAPA HiFi HotStart Ready Mix PCR Kit (Catalog: 0370, KAPA BIOSYSTEMS) and purified using AMPure XP beads.Fungal ITS2 region amplified using ITS3mixF and ITS4R and Bacterial 16S region amplified using 341F and 785R  primers. Incorporation of specific barcodes and library generation according to the manufacturers protocol (Illumina, USA).Libraries quantified using a Qubit fluorometric assay, normalized based on their concentrations and purified using AMPure XP beads.For the estimation of absolute abundance, quantification of the purified final libraries performed using an in-house developed qPCR assay.Amplicon libraries sequenced on an Illumina MiSeq Next Generation Sequencer in a shared run using the Illumina V3 2*250 paired-end sequencing.