ssRNA-Seq of FC02 induction in PAO1
The second strand of cDNA was marked by incorporating dUTP during the synthesis.  cDNA fragments were adenylated at 3ends, and indexed adapter was ligated to cDNA fragments.  Limited cycle PCR was used for library enrichment. The incorporated dUTP in second strand cDNA quenched the amplification of second strand, which helped to preserve the strand specificity.