NextSeq 550 sequencing
4C seq in BJ1-WT mESC using viewpoint located on a CTCF binding site upstream of Dlk1. Ligation events from both alleles are amplified. SNP to discriminate alleles: G (JF1, paternal) and A (Bl6, maternal) 4 nucleotides 3â€™ of the 4C forward primer.