wastewater biofilm RNA seq
From rRNA-depleted RNA, directional libraries were prepared using the TruSeq Stranded mRNA Sample preparation kit following the manufacturer's instructions (Illumina). Libraries were quantified on Bioanalyzer DNA chips (Agilent) and Qubit dsDNA HS Assay Kit (ThermoFisher). 150 bp paired read sequences were generated on the Nextseq 500 (high output) sequencer according to manufacturer's instructions (Illumina). The multiplexing level was 10 samples per lane. Reads were cleaned for low-quality and adapter sequences using Cutadapt version 1.11.