MICROBIAL-DERIVED IMMUNOSTIMULATORY SMALL MOLECULE SYNERGIZES WITH ANTI-PD-1 THERAPY IN LUNG CANCER
Cultures were pelleted by centrifugation at 4000 rpm for 10 minutes. The MagAttract HMW DNA kit (QIAGEN) was used to isolate DNA from culture pellets from each strain per manufacturer instructions. Following DNA isolation, the PowerClean DNA  Clean-Up Kit (MoBio) was used to further purify the high molecular weight DNA. Cleaned DNA was submitted to the University of Florida Interdisciplinary Center for Biotechnology Research (UF-ICBR) for long read sequencing using the PacBio SMRT system (Pacific Biosciences, Menlo Park, CA).