RNA-Seq of Severe acute respiratory syndrome coronavirus 2: Human NJ-BioR-232
The NJ-BioR-232 library was prepared using the KAPA Hyper Library Preparation Kit + RiboErase, HMR (Roche), with up to 200ng input, unique dual indexed adapters (Illumina), enriched with 12 cycles of PCR. The final libraries were cleaned up with bead-based size selection, quantified by PicoGreen (Life Technologies) and their fragment size distribution assessed on the Fragment Analyzer (Agilent), and sequenced on Illumina NovaSeq 6000 with 2x150bp reads targeting 60M read coverage.