DNA-Seq of Salmonella enterica: food
NEBNext Ultra DNA Library Prep Kit for Illumina was used throughout the process following the manufacturers recommendations. The genomic DNA was fragmented (Covaris S220). Fragmented DNA was cleaned up and end repaired. Adapters were ligated after adenylation of the 3ends followed. DNA libraries were validated (Qubit 2.0 Fluorometer). The DNA libraries were also quantified by real time PCR.