Treatment with 3 fasting days and 7 satiety days
Penaeus vannamei post-larvae were exposed to different stress periods with fasting for 3, 7, and 14 days at three temperatures each (22C, 26C, and 30C), followed by a recovery stage of feeding ad libitum and temperature at 30C for the remaining time of five experimental weeks. Treatments were compared with a control group fed to satiety and at 30C throughout the experiment. Growth performance was determined and muscle was sampled for RNA-Seq analysis.