RNA-Seq of Rubus idaeus: Seed 2 DPA
To isolate RNA, each tissue was homogenized in the presence of liquid nitrogen and total RNA was extracted using the Cetyl Trimethyl Ammonium Bromide (CTAB) RNA extraction Method. The RNA samples were further purified and concentrated by Macherey-Nagel NucleoSpin RNA Clean-UP XS kit (USA), and send for RNA-seq on Illumina  HiSeq 4000.