MDA-MB-231 resistant to paclitaxel BRG1 ChIP-Seq
ChIP-Seq: Cells were cross-linked with 1% formaldehyde (Merk) in PBS for 10 min at room temperature. BRG1 was immunoprecipitated with anti-BRG1 antibody (#49360 Cell Signaling Technology) and Dynabeads Protein G (Thermofisher Scientific) as previously described (Biomed Pharmacother. 2023 Dec;168:115798. doi: 10.1016/j.biopha.2023.115798). Libraries were constructed using NEBNext Ultra II DNA Library Prep Kit for Illumina with SPRIselect beads for clean-up and size-selection steps (<350 bp), and NEBNext Multiplex Oligos for Illumina (Index Primers Set 3) (New England Biolabs) according to the protocol.