mature mud metagenomic: P-4 replicate 1
Microbial composition was determined by amplifying the hypervariable V4 region of bacterial and archaeal 16S rRNA gene, using forward and reverse degenerate primers 515F-806R. Amplicons were purified through AMPure XP beads (Beckman Coulter, Brea, CA, USA), libraries were prepared with Illumina DNA Prep with IDT for Illumina DNA/RNA UD Indexes Set C (Illumina Inc, San Diego, CA, USA). Sequencing was then performed with Illumina NovaSeq 6000 platform (2x150bp, paired-end).