V3V4 amplicon sequencing of UV-B tolerant bacteria
16S rRNA PCR product was further used to amplify V3-V4 region (~465 bp) using specific primers (V3V4F 5'CCTACGGGNGGCWGCAG'3; V3V4R 5'GACTACHVGGGTATCTAATCC'3, https://help.ezbiocloud.net/16s-rrna-and-16s-rrna-gene/). The amplified product was then cleaned up using AMPure XP beads (Beckman Coulter, USA). The cleaned product was taken for amplicon sequencing with the Illumina MiSeq platform. A single library was prepared using the NEBNext Ultra DNA library preparation kit for Illumina (New England BioLabs Inc, USA) according to manufacturer instructions. The prepared library was quantified using Qubit HS assay (Invitrogen, USA) and then loaded for cluster generation and sequencing on Illumina MiSeq to generate 2X250 bp sequence reads.