RNA-seq of Solanum lycopercicum: pericarp tissues at mature green to breaker stages
Tomatoes at the mature green to breaker stage were treated with ethylene (ET), gibberellin (GA), or both (GA+ET). For ET treatment, the fruits were dipped in 1 mM ethephon (Inbio Corp, Jecheon, South Korea) solution for 5 min under vacuum at 30 kPa. For GA treatment, the fruits were dipped in 0.5 mM GA3 solution [prepared in ethanol/distilled water (1:1000, v/v) containing 0.1% (v/v) Tween-20; Sigma-Aldrich, St Louis, MO, USA] for 15 min. The fruits were sequentially treated with GA, air dried, and then treated with ET for the combined treatment. The fruits treated with sterile water served as control samples. Following treatment, the fruits were kept in darkness at 20  2 C with 90%  5% relativity humidity (RH) for 10 days. RNA sequencing was performed using pericarp tissues 1 d after hormone treatment.