Whole genome sequence of Psuedopithomyces maydicus
We obtained an isolate from brewery wastewater by culturing on solid Yeast ExtractPeptoneDextrose (YPD) agar media at 30oC for 2 days. The colony was isolated and streaked out on a new YPD plate. Taxonomic classification was made via Sanger sequencing of the D1/D2 domain of the large-subunit (28S) ribosomal DNA (NCBI BLASTN webserver against the NCBI nr/nt database in megablast mode with top hit annotated to a P. maydicus partial 28S sequence, MF919633.1, at 99% identity; see Supplementary Figure 1 for alignment; https://blast.ncbi.nlm.nih.gov/Blast.cgi, executed on 06/31/2022; available from Zenodo via https://doi.org/10.5281/zenodo.7374666). Genomic DNA was extracted using liquid nitrogen and mechanical grinding of the fungal culture followed by application of the Qiagen DNeasy PowerSoil Pro Kit.  1.5g of input DNA was subjected to shearing (Megaruptor3, Diagenode Inc, Denville, NJ, USA; operated for 20kb target at speed 35) and then 800ng sheared DNA was used to construct a sequencing library using the SQK-LSK109 ligation sequencing kit (Oxord Nanopore Technologies Ltd, Oxford, UK), barcoded using the EXP-NDB104 native barcoding kit (Oxford Nanopore Technologies; barcode 10). Following construction, 200 ng of the library was sequenced on a GridION instrument (Oxford Nanopore Technologies; release 21.05.20) for 72 hours. Basecalling was performed using Guppy 5.0.13 (Oxford Nanopore Technologies) in SUP mode.