KOD DNAP 5pt library pre-selection
The 5-point saturation mutagenesis library targeting L403, D404, F405, L408 and Y409 was generated using primers KOD_5ptSat_408-409_F1 (AGATCCNNKNNKCCCTCAATCATC) and KOD_5ptSat_403-405_R1 (KNNKNNKNNGTACACTATGTTCTCCCAC) on an exonuclease deficient KOD DNAP (D141A/E143A) background (KOD exo-) through inverse PCR (iPCR).