RNA-Seq of Crassostrea ariakensis: adult female gonads
TRIzol reagent (Invitrogen, USA) was used to extract total RNA following the manufacturer's instructions. A Qubit RNA Assay Kit with a Qubit 2.0 Fluorimeter (Invitrogen, USA) was used to quantify RNA, and the RNA integrity was then evaluated using an RNA Nano 6000 Assay Kit and a Bioanalyzer 2100 system (Agilent Technologies, CA, USA).