RNA-seq of Oryza sativa subsp. indica: leaf, control sample
Total RNA was extracted from drought-stressed MR 219 and control plants using a CTAB (hexadecyl-trimethyl-ammonium bromide) method, followed by DNase treatment. The integrity and concentration of RNA were determined using agarose gel electrophoresis and a nanophotometer (Implen GmbH, Germany). RNA integrity number (RIN) was assessed using a bioanalyzer (Agilent 2100, United States). RNA samples that passed the quality test were sent to Beijing Genomics Institute (BGI), China, for RNA sequencing (RNA-Seq). Only two biological replicates were sent for control and drought-stressed samples (40%_SMC and 30%_SMC).