16S metagenomics of Spodoptera litura gut
The amplicon from each sample were purified with ampure beads to remove unused primers and an additional 8 cycles of PCR was performed using illumina barcode adapters to prepare the sequencing libraries. Libraries were purified using ampure beads and quantification was done using Qubit dsDNA high sensitivity assay kit. Sequencing was performed using illumina miseq with 2x300PE v3v4 sequencing kit.