RNA-Seq of Brassica napus: phloem tissue laser dissected from hypocotyl of mock inoculated plants 7 dpi
Transverse sections were made for tissue from three plants, from these between 2000 and 10000 phloem areas were dissected using a Leica LMD7. RNA was extracted using Arcturus PicoPure. Ribosomal RNA was removed from total RNA then cDNA was made using random hexamers and a directional library was prepared for paired-end sequencing.