Shotgun metagenomic sequencing of bovine teat apex microbiome
DNA was extracted from each sample using the PowerSoil Pro Kit (Qiagen, Hilden, Germany).  The extraction of DNA was automated on the QiaCube Connect instrument (Qiagen, Hilden, Germany).  Libraries were prepared using the Illumina Nextera XT DNA Library Preparaton Kit (Illumina Inc., San Diego, CA).  Libraries were then sequenced on an Illumina NovaSeq 6000 instrument (Illumina Inc., San Diego, CA) using an S4 flow cell (2x150 base pairs).