H3K27ac CUT&Tag library of O18
CUT&Tag libraries were constructed.Chondrocytes were lysed to acquire nuclei and bind to Concanavalin-coated magnetic beads. Beads were incubated successively with the primary antibody, the secondary antibody and pG-Tn5 loaded with mosaic-end adapters. Tn5 was activated by addition of Mg2+. DNA was released in a small volume of SDS. Samples were enriched by PCR amplification followed by magnetic bead cleanup and sent for paired-end 150-bp sequencing performed on an Illumina HiSeq 2500 platform.