WES and RNAseq of Triple negative breast cancer patient tumors: Sample M1510624_1
"For this, strandspecific, poly-A+ RNA-Seq libraries for sequencing on the Illumina platform were prepared as previously described (PMID: 25360585). Briefly, poly-A+ mRNA was extracted from 1 ?g total RNA, followed by fragmentation and first strand cDNA synthesis. The resultant cDNA was endrepaired, A-tailed and ligated with Illumina Dual barcode adapters."