Speg-CKO
Skeletal muscles from quadriceps femoris were isolated from WT and Speg-CKO mice. A total of 8 mRNA samples (n = 4 for per group) was isolated and was then converted into double-stranded DNA (dsDNA). Procedures including the sample quality control (QC), the library preparation, and sequencing for all the samples were performed by Novogene (Sacramento, CA, USA). RNA-seq raw data were aligned using align (Rsubread, V2.0.1) and the latest UCSC mouse annotation (GRCm38/mm10). The raw data were trimmed before aligned using Trimmomatic (V0.39) for QC. Gene-level read counts were quantified using featureCounts (Rsubread, V2.0.1). To identify differentially expressed genes, DESeq2 (version 1.26.0) was used with default parameters in Bioconductor packages. All software/packages were run using their default parameters. Count tables were normalized to TPM (Transcripts per Million) for visualizations and QC.