RNA-Seq of Brassica napus: phloem tissue laser dissected from hypocotyl of mock inoculated plants 12 dpi
Transverse sections were made for tissue from three plants, from these between 2000 and 10000 phloem areas were dissected using a Leica LMD7. RNA was extracted using Arcturus PicoPure. Ribosomal RNA was removed from total RNA then cDNA was made using random hexamers and a directional library was prepared for paired-end sequencing.