RNAseq of Pinus strobiformis-Pinus flexilis hybrid seedlings
Total RNA was extracted using Spectrum Plant Total RNA Kits (Sigma-Aldrich). Following polyA tail selection to enrich for mRNA, libraries were prepared following standard protocols for the NEBNext Ultra II RNA Library Prep (Illumina) and sequenced on the Novaseq6000 platform at Novogene Corporation (Sacramento, CA).