DNA-Seq of a potable water reuse system: Miramar_Reservoir_A
Samples were concentrated down to 500 mL from large volumes using tangential flow filtration with a 100 kDa (NMWC) hollow fiber ultrafiltration cartridge. The concentrated sample was further concentrated using 0.22-m polyethersulfone membrane Sterivex filters, then DNA was extracted from the Sterivex filters using lysis buffer and proteinase K on overnight rotation at 55C, and lysate was purified using a modified NucleoSpin Tissue protocol. DNA was mechanically sheared to produce 350bp fragments for sequencing and the Accel-NGS 2S Plus DNA Library Kit was used to prepare libraries on 50 ng of the fragmented DNA.