Illumina MiSeq of microbes in the soilpetroleum hydrocarbon contaminated soil
1) Add the Illumina official adapter sequence to the outer end of the target region by PCR; 2) Use the gel extraction kit to cut the gel to recover the PCR product; 3) Tris-HCl buffer elution, 2% agarose electrophoresis detection; 4) Hydrogen Sodium oxide denatures, resulting in single-stranded DNA fragments. Reagents: TruSeqTM DNA Sample Prep Kit