miRNA-seq of Yorkshire: high sperm motility seminal plasma extracellular vesicles
We evaluated the sperm motility phenotypic data of 230 boars by using a computer-assisted sperm analysis from a national boar station. Twelve Yorkshire boars with high sperm motility and twelve Yorkshire boars with low sperm motility were selected for SPEV extraction. All the individuals were sexually mature, aged between 14 and 36 months old. The average total sperm motility of H group was 0.94, while that of L group was 0.40. For each boar, 60 mL of semen plasma was used to extract EVs. Deep RNA sequencing was performed to gain insight into the types of RNA in SPEVs, including mRNAs, miRNAs and lncRNAs.