DNA-Seq of cerebral spinal fluid:adult female
Take 600 ul CSF sample  after mixing and shaking with glass beads, DNA was extracted using the TIANamp Micro DNA kit (DP316, Beijing Tiangen Biochemical Technology Co., Ltd.) according to the instructions of the kit. Extract DNA for DNA library construction.Insert fragment size using Agilent 2100 Bioanalyzer quality control library, control DNA library concentration using Qubit dsDNA HS kit (Thermo Fisher Scientific, USA), and cyclize to form a single stranded circular structure. The cyclized library is replicated by rolling rings to generate DNB nanospheres. The prepared DNB nanospheres were loaded onto the sequencing chip and sequenced using Illumina HiSeq 3000