13D_Pythium_CGMMV_rep1
Six days after sowing, 300 seedlings were transplanted into P. spinosum-inoculated medium, and an additional 100 seedlings were transplanted into non-inoculated control medium. Each cucumber seedling was transplanted to a 100 ml pot containing 100 g vermiculite and grown in greenhouse conditions described above. Five days after the P. spinosum inoculation, which permitted observation of the primary "damping off" of seedlings due to Pythium alone (Philosoph et al., 2018), 100 symptomless plants were taken to the core experiment, along with the 100 non-inoculated plants. Fifty plants from each group were then inoculated with CGMMV as described above, forming four different treatments: (i) plants infected with P. spinosum inoculated with CGMMV (PS+CG), plants with P. spinosum (PS); (iii) plants inoculated solely with CGMMV (CG), and (iv) non-inoculated healthy control plants (C).