RNA-Seq of human CSF: preterm infants with GM-IVH
CSF was centrifuged to remove cellular material. HTG EdgeSeq Plasma Lysis Buffer was added to lyse the sample making RNA available to subsequently bind to corresponding target specific nuclease protection probes. Then S1 nuclease was added. After S1 digestion was terminated, each processed sample was used as a template to set up PCR reactions. The library was quantified in accordance with OP-00079 HTG EdgeSeq KAPA Library Quantification for Illumina Sequencing. PhiX control adaptor-ligated library was used. The sequencing was performed on the Illumina MiSeq sequencer.