Ophiomastix wendtii specimen 3, tissue sample 6, inner arm core
The RNA-Seq libraries were prepared using the Illumina Stranded mRNA Prep kit (Illumina, USA), following Illumina Stranded mRNA Prep Reference Guide. The fragmentation was enzyme-based. We conducted quality control on the final libraries to assess RNA concentration with a Qubit dsDNA BR (broad range) assay kit and quality by Tapestation 4200 analysis using the High Sensitivity D1000 Screentape (Agilent, USA). We prepared 16 Illumina paired-end read sequencing libraries to account for both the biological and technical replicates.