Iso-Seq of angii-induced raecs
PolyA+ RNA after double-stranded cDNA preparation, PCR amplification, product purification with AMPurePB magnetic beads, verification of DNA size, followed by qualitative and quantitative analysis using a Bioanalyzer.  The resulting double-stranded DNA was used to generate the SMRTbell libraries using PacBio Template Prep Kit. SMRTbell templates are then sequenced in on the PacBio Sequel System.