Sperm transcriptome of precocious Eriocheir sinensis (Biological Repeat 3)
Spermatozoal RNA was extracted using Trizol reagent. mRNA was enriched using poly-T oligo-attached magnetic beads. Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA). The reads were then mapped back to contigs with paired-end reads.