Aquaculture bacteria
The genomic DNA was randomly sheared into short fragments. The obtained fragments were end repaired. A-tailed and further ligated with Illumina adapter. The resulting fragments with adapters were size selected, PCR amplified unless otherwise specified as PCR-free, before proceeding for purification. The library was quantified through Qubit and qPCR, and size distribution detected with fragment analyzer. Quantified libraries were pooled and sequenced on Illumina platforms according to the effective library concentration and required data amount.