RNA-seq of duck small intestine
a ribosomal RNA (rRNA) removal method was used according to the manufacturer's recommendations, using 1g of total RNA per sample to generate sequencing libraries via the NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, Ipswich MA). The sequencing libraries were then diluted to 1ng/l and the insert size of the libraries was around 250-300 bp. Finally, sequencing was performed using the Illumina NovaSeq 6000 (Illumina, San Diego, CA) platform to generate pairing reads of 150 bp