GSM8396148: HeLa HP1 knockdown biological rep 2; Homo sapiens; Hi-C
Genomic DNA was extracted from 500,000 cells Crosslinking was carried out with 1% formaldehyde for 10min at room temperature. Nuclei were permeabilized. DNA was digested with 100 units of MboI. After reversal of crosslinks, ligated DNA was size selected between 200-1000 bp and subsequently prepped for Illumina sequencing.