GSM6734249: Liver_CON_NASH_1 [A54N]; Mus musculus; RNA-Seq
RNA were extracted from frozen liver samples using TRIzol following manufacturer's instruction. Ribosomal RNA was removed with the FastSelect HMR kit (Qiagen). The RNAseq libraries were prepared with the Kapa Hyper Stranded mRNAseq Sample Prep kit' (Roche). The libraries were pooled; quantitated by qPCR and sequenced on one SP lane for 101 cycles from one end of the fragments on a NovaSeq 6000.