RNAseq of imipenem treated Pseudomonas aeruginosa
Samples were DNAse treated with Invitrogen DNAse (RNAse free). Library preparation was performed using Illuminas Stranded Total RNA Prep Ligation with Ribo-Zero Plus kit and 10bp IDT for Illumina indices. Sequencing was done on a NextSeq2000 giving 2x50bp reads. Demultiplexing, quality control, and adapter trimming was performed with bcl-convert (v3.9.3).