GSM8283457: control, day 7, replicate 2, Aggrewell; Homo sapiens; RNA-Seq
Total RNA was extracted using Qiazol, chloroform, isopropanol method. Five or more organoids were pooled in each sample. QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen) with single i7 indexing, equimolar pool 60nM