GSM7416845: Scrambled control, rep2; Mus musculus; RNA-Seq
The RNeasy mini kit was purchased from Qiagen, (Maryland, USA) and utilized to isolate RNA from cell adipocytes. The library was created using the Illumina Stranded Total RNA Prep protocol. The library was amplified using PCR and cleaned with Agencourt AMPureXP (Beckman, CA,USA). The final library was then normalized, pooled, and loaded into an SP flow cell for up to 400 million reads of coverage. On an Illumina NovaSeq 6000 platform, the library was sequenced (200 cycles).