ITS gene amplicon sequencing of sediemnts from the South Col of Mount Everest: SC3
The internal transcribed spacer of the fungal ribosomal RNA (rRNA) operon was amplified using its1-f/its2-r primers. Each primer combination had a unique barcode corresponding to a unique sample. Barcodeded amplicons were normalized and pooled into a single library which was sequenced on a 2x250 Illumina MiSeq run. Sequences were were subsequently demultiplexed by barcode into individual files corresponding to each sample.