DNA sequencing of the RP4 plasmid
The RP4 plasmid was extracted from 20 ml of the overnight culture of E. coli J53/RP4 using the Plasmid Mini AX kit (A&A Biotechnology) according to the manufacturers instructions. The DNA library was prepared using the Nextera XT DNA Library Preparation Kit, indexed with the Nextera XT Index Kit v2 Set A and sequenced on the Illumina 2250-base paired-end v2 MiSeq platform.