Benin 2023 ASFV Samples
Total nuclei acid was extracted using MagMax Core kit (Applied Biosystems), and used for qPCR targeting p72 using a modification of Zsak et al (2005). Libraries were constructed with the Nextera XT kit (Illumina) and sequenced with a paired end 300 cycles NextSeq 2000 kit on a NextSeq 2000 Illumina platform.  For Nanopore, ASF Tile amplicons were prepared using the LongAmp Taq DNA polymerase (NEBiolabs)(Warr et al2021), libraries were prepared with the Rapid BC Kit v 14 (ONT), loaded multiplexed in a R10.4 flow cell (FLO-MIN106) and run for 72 hours on a GridION platform.