C3-Genome OmniC
The Dovetail Omni-C library was constructed and sequenced by Arizona Genomics Institute (The University of Arizona, USA) by using standard protocols (Dovetail Genomics, Dovetail Omni-C Kit Catalog #21005). One gram of flash-frozen leaf tissue of the same I. puberulus individual was ground using a SPEX Sample Prep Freezer Mill, for three rounds at 1:15 minutes, 13 CPS. Nuclei isolation was performed using the Circulomics LN2 Plant Tissue Protocol (Circulomics NUC-LNP-001). The nuclei pellet was resuspended in 1X PBS, aliquoted into three tubes and centrifuged at 6000 rpm for 5 min. The supernatant was then discarded and the nuclei pellets flash frozen for storage at -80oC. One aliquoted nuclei pellet was then processed using the mammalian cell protocol with Dovetail Omni-C protocol mammals V1.3. Completed libraries were sequenced on an Illumina NovaSeq 6000 PE150.