GSM6433164: QKI-KD-3; Rattus norvegicus; RNA-Seq
Cells were lysed with TRI-reagent and total RNA purified by Direct-zol purification column (Zymo Research). The DNase treatment was performed in column during the total RNA purification. We have used the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (#E7760L) and the NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs) (#E6440L) in conjunction with NEBNext® Poly(A) mRNA Magnetic Isolation Module (#E7490L) for these samples.