GSM8098350: Fibroblasts from No Fibrosis patient 05; Homo sapiens; RNA-Seq
Total RNA from fibroblast strains was isolated and purified using the RNeasy Plus Mini kit (Qiagen), according to the manufacturer's instructions. Total amount and integrity of RNA samples were evaluated by NanoDrop One (Thermo Fisher Scientific) and Bioanalyzer System (Agilent technologies). The integrity criteria were values between 1.8 and 2.2 (A 260/280), >1.8 (A260/230), and RIN ≥ 8. RNA sequencing of patient fibroblasts was performed at the CEA Genomic Center (CNRGH, Evry, France). RNA libraries were prepared using the Illumina Truseq RNA Library Preparation Kit V2 according to the manufacturer's instructions. Paired-end RNA sequencing was performed on HiSeq4000 (Illumina) with an input of 1 µg, with 100 bp paired-end reads. A single sequencing run was performed per patient. For sequencing after VIM-AS1 knockdown, RNA sequencing was performed at the Curie Institute NGS/Bioinformatics core facilities. An Illumina Stranded mRNA Prep Kit was used for library preparation, followed by paired-end RNA sequencing on an Illumina NovaSeq System, with an input of 1 µg, with 100 bp paired-end reads.  A single sequencing run was performed per biological replicate.