GSM7720881: CEL_seq_ctrl_siRNA_EV_rep2; Homo sapiens; RNA-Seq
Total RNA was extracted using TRI reagent (BioLabs) according to the manufacturer specifications. RNA concentration was measured using Qubit Flex Fluorometer (ThermoFisher Scientific). RNA sequencing libraries were prepared using the CEL-Seq2 protocol in the Technion genomic Center (TGC), as published by (Hashimshony et al., 2012), with one modification; instead of single-cells as input, 2 ng purified RNA was taken as input for library preparation. The CEL-Seq2 libraries were analyzed for average fragment size using Agilent 2200 TapeStation (Agilent) and concentration was measured using Qubit Flex Fluorometer (ThermoFisher Scientific). The libraries were sequenced on the Illumina NextSeq 2000 sequencer (Illumina), 12 bases for read 1 and 88 bases for read 2. Demultiplexing was performed in two steps as published by (Hashimshony et al., 2012) CEL-seq