RNF113A KO HeLa cells rep2
Total RNA was extracted using a Hybrid-RTM RNA extraction kit (GeneAll, 305-101), according to the manufacturers instructions, and RNA quality was confirmed using a Bioanalyzer 2100 system (Agilent Technologies). To prepare the RNA libraries, 1 g of total RNA was processed using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, RS-122-9005DOC). The libraries were sequenced using Illumina NovaSeq