GSM6775407: iNeuron, RBM12 knockdown, no drug, rep2; Homo sapiens; RNA-Seq
RNA was extracted using the Rneasy Plus Mini Kit (Qiagen, Cat. #74106) and NEBNext Poly(A) mRNA magnetic isolation module (New England Biolabs, Cat. #E7490). 1 ug RNA and 500 ng RNA were used as input for library generation for HEK293 and neurons, respectively. NEBNext Ultra RNA Library prep Kit for Illumina or NEBNext Ulta II Directional RNA Library Prep Kit for Illumina were used according to the manufacturer's instructions.