GSM8240583: cSAM-Setdb1 sg1, bio rep 2; Mus musculus; RNA-Seq
Total RNA of 2 x10^6 cultured Setdb1-depleted or non-targeting control cSAM cells in each sample was extracted by RNeasy Mini Kit (QIAGEN). rRNA depletion stranded Library. Ribosomal RNA was removed by RNase H. After RNA fragmentation, cDNA was synthesized by random hexamer-primed reverse transcription, followed by a second-strand cDNA synthesis with dUTP. Following end repair, add A and adaptor ligation, the DNA fragments were amplified by PCR.