soil15
DNeasy PowerSoil Pro Kit (Qiagen, Germany) was used to extract genomic DNA from the soil samples according to the manufacturer's protocol. DNA quality and quantity were measured using a NanoDrop UV-Vis spectrophotometer and a Qubit fluorometer 2.0 (Thermo Fisher Scientific, USA). An NGS library was prepared by amplifying the V4-V5 region of the small subunit ribosomal RNA (16S rRNA) gene for bacteria using 515F (ACA CTC TTT CCC TAC ACG ACG CTC TTC CGA TCT GTG NCA GCB GCC GCG GTR A) and 907R (GTG ACT GGA GTT CAG ACG TGT GCT CTT CCG ATC CGY CWA TTY HTT TRA GTT T) primers. Library quality was evaluated using an Agilent 2100 Bioanalyzer (Agilent Technology, USA). Lastly, sequencing was carried out at the next-generation sequencing (NGS) core facility of Kyungpook National University (Daegu, South Korea) and using an Illumina MiSeq platform (Illumina, USA) with single-end 250 bp reads.