GSM8007982: PC-9_YVMA-5_DMSO_24h_in_vitro_rep1; Homo sapiens; RNA-Seq
In vivo: Frozen tumours were cut into 5x5 mm pieces and ground using one steel bead (#69989 Qiagen) per 2 mL vial in 1 mL Trizol using a tissue lyser II (Quiagen) at 4 °C (1/s frequency, 30 sec, 30 cycles). Lysates were processed using the RNeasy Plus Universal Mini Kit (Qiagen Cat No 73404) following the manufacturer's instructions. ; In vitro: After treatment, cells were washed with ice cold PBS and RNA extracted using the RNeasy Plus Universal Mini Kit (Qiagen Cat No 73404) following the manufacturer's instructions using 1 mL Trizol per 6-well. RNA was eluted in 50 µL of ddH2O for further processing Quant-seq libraries were prepares using the QuantSeq 3' mRNA-Seq Library Prep Kit for Illumina from Lexogene according to manufacturer's instructions.