GSM7814089: Mouse heart macrophages, replicate3; Mus musculus; RNA-Seq
Laser capture micro-dissected muscle tissues or macrophages sorted from muscles, hearts and lungs were processed for RNA extraction. RNA was extracted by using RNeasy micro-kit and DNase on-column digestion (Qiagen) following the manufacturer's guidelines. RNA with a RIN factor above 8 was used for library preparation. cDNA libraries for each sample were generated at Source Bioscience Laboratory (Cambridge, UK) by using NEBNext Single Cell/Low Input Library Prep Kit (Ilumina). read length 150 bp