Cefoxitin-Treatment-64ug/ml
Fresh overnight culture of C. perfringens was added to 6 McCartney glass bottles containing, each supplemented with an antibiotic to reach a final concentration of   MIC and a control without any antibiotic. All treatments were then incubated anaerobically at 37C with an OD600 of 0.3. This process was repeated to achieve 3 technical replicates for each antibiotic treatment.