GSM8008893: SOX9 ChIP-seq; Homo sapiens; ChIP-Seq
For ChIP-seq 10 million cells per experiment were harvested and crosslinked with 1% formaldehyde. Crosslinked cells were lysed and homogenized using a Dounce homogenizer. Nuclei were lysed and sonicated to shear chromatin. Chromatin was incubated with antibody and beads. For ATAC-seq 100 000 cells per experiment were harvested and lysed and nuclei pelleted. Nuclei were incubated with Tn5 transposase. For ChIP seq NEBnext DNA Ultra ii (New England Biolabs) was used to generate libraries according to the manufacturers protocol. For ATAC-seq libraries were generated generated using Ad1_noMX and Ad2.1–2.4 barcoded primers from Buenrostro et al. 2013 and NEBNext High-Fidelity 2X PCR Master Mix for a total of 10 cycles.