GSM6914110: P0 Six2TGC; Tsc1-1; Mus musculus; Hi-C
Isolated kidneys were digested with 2.5 mg/ml collagenase D at 37 degrees celsius for 20-25 minutes. NPCs were sorted by FACS (Sony SH800S) on the basis of GFP-positivity. Libraries were prepared using Arima-HiC Kit, low input protocol (Arima Genomics) and Accel-NGS 2S Plus DNA Library Kit (Swift Biosciences). Libraries were pooled into a single sample and sequenced on an Illumina NovaSeq 6000 using 150bp paired-end sequencing.