GSM7093628: Sample 2_G0Tg26; Mus musculus; RNA-Seq
Nuclei from isolated five glomeruli sample types (WT, G0xTg26, G1xTg26, G0+interferon-g, G1+interferon-g) were prepared. Snap frozen isolated glomeruli were lysed in EZlysis buffer (#NUC101-1KT, Sigma, Darmstadt, Germany) and homogenized 30 times using a loose Dounce homogenizer and 5 times in a tight pestle. After 5 min incubation, the homogenate was passed through a 40 µm filter (43-50040, PluriSelect, El Cajon, CA) and centrifuged at 500g at 4°C for 5 min. The pellet was washed with EZlysis buffer and again centrifuged at 500g at 4°C for 5 min. The pellet was resuspended with DPBS with 1% FBS and passed through a 5 µm filter (43-50005, PluriSelect) to make final nuclei prep for loading on to a 10x Chromium Chip G (10X Genetics, Pleasanton, CA) for formation of gel beads in emulsion (GEM). 10x Genomics Chromium 3' V3.1 mRNA-seq library protocol