GSM6395012: Pigmented peripheral retina,, scRNAseq; Mus musculus; RNA-Seq
Freshly collected  E13.5 optic cups  were pooled from 3 embryos each for pigmented and albino mice. Cells were dissociated using a Papain digestion protocol. Cells were FAC sorted using TdTomato and single cell suspension was collected in DMEM with 10% FBS for sequencing. Library was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics) for 3' gene expression profiling