GSM6784589: TC28a2 cells transfected with control vector, replicate 3 [TC28_ZEB1_DRUGseq]; Homo sapiens; RNA-Seq
Total RNA was isolated from TC28a2 cells transfected with a vector control (n=3) or ZEB1 overexpression vector (n=3) 48 hours post-transfection using TRIzol. RNA quality was confirmed using NanoDrop measurements of OD 260/280 and 260/230. DRUG-seq (high throughput low-cost RNA-Seq) libraries were generated for all replicas and the libraries sequenced on Illumina NextSeq 2000.