Whole exome sequencing of Homo sapiens: Melanoma tumor, Skin (thigh)
Extraction of DNA from the isolated melanoma metastatic brain tumor tissue was performed using the Gentra Puregene Tissue Kit (QIAGEN, CAT # 158066) according to the manufacturer's protocol. Extracted DNA was quantified using Qubit (Thermo Fisher Scientific).  DNA-seq libraries were prepared for each sample using the Kapa Hyper Prep kit (Roche, Cat # KK8504) and indexed using IDT for Illumina DNA unique dual indexes (UDI) Set A. Final libraries were quality checked using 2100 Bioanalyzer (Agilent Technologies) and Qubit 2.0 (ThermoFisher Scientific) and pooled in batches of 12 (pre-capture pooling).  Each pool of 10 libraries were then hybridized with IDT Human xGen Exome Research Panel V1 probes (IDT, CAT # 1056114) to capture and pull down the portion of the DNA-seq library representing the human the exome. Final captured libraries were amplified, pooled into 1 library, and sequenced using 2 lanes of an Illumina NovaSeq 6000 S4 flow cell, generating 150bp PE reads. Sequence data was demultiplexed and Fastq files generated using Illumina Bcl2Fastq conversion software.