GSM8007850: MF317_A2_GEX_zebrafish_multiseq; Danio rerio; RNA-Seq
Each kidney marrow was split into four portions, labelled with lipid anchor plus individual barcode solution (2 μM) for 5' on ice and then incubated with lipid co-anchor (2 μM) for 5' on ice. Each cell portion was individually FACS-sorted to obtain one population (mmp9 NO, INT, HI or WKM). All cells were gated on live gate, for WKM debris was excluded in a FSC/SSC gate, and mmp9 NO, INT, HI were gated on LysC:CFP positivity and different levels of Mmp9:Citrine-CAAX expression. Single cell suspensions were immediately subjected to scRNA-seq using the Chromium Single Cell Controller and Single Cell 3' Library & Gel Bead Kit v3.1 (10x Genomics, Pleasanton, CA), according to the manufacturer's protocols (10x Genomics) and sequenced by protocol.