Sequencing of multidrug-resistant P. aeruginosa: Isolate F2258
Library preparation was carried out following an Illumina protocol: DNA was randomly fragmentated by sonication aiming for a size of 350 bp. Fragments were then end repaired, A-tailed and Illumina sequencing adapters were ligated. PCR amplification was carried out and an Ampure XP bead-based purification was performed.