Library with 70k cells loaded
Nuclei were isolated from middle frontal gyrus tissue using nuclei isolation buffer followed by an additional wash with nuclei isolation buffer. They were then distributed to 30,000 per well and tagmented with indexed tagmentation complexes. Tagmented nuclei were pooled, spun down and then resuspended in 10x ATAC loading buffer prior to loading 70,000 nuclei on a 10x Genomics scATAC kit channel.