16s of bacteria:Stipa krylovii
Soil bacterial communities were analyzed using high-throughput amplicon sequencing. Bacterial 16S rRNA gene V3-V4 region was amplified using primer 338F (5- ACT CCT ACG GGA GGC AGC AG -3) (Lane, 1991) and 806 R (5-GGA CTA CHV GGG TWT CTA AT-3) (Caporaso et al., 2011; Walters et al., 2016)