GSM8240332: Pero-F-3_tick; Peromyscus leucopus; RNA-Seq
After all rodent samples were collected, samples were thawed on ice, transferred to QIAzol, and bead beaten with a 6.35 mm chrome steel bead (Biospec Products) for two cycles of two minutes each at an oscillation frequency of 30/second using a TissueLyser II (Qiagen). RNA was extracted using the miRNeasy mini kit (Qiagen), with the modification that two RWT washes were performed. gDNA was digested using TURBO DNase (Invitrogen) and RNA was repurified using the RNeasy MinElute Cleanup Kit (Qiagen). Purified RNA was submitted to Azenta Life Sciences/Genewiz for library preparation and sequencing (Illumina HiSeq 2x150bp).