GSM6398520: siRNA-Mettl3+CSE.Input; Homo sapiens; RIP-Seq
Total RNA was extracted from the two groups of cells by using TRIzol Reagent (Invitrogen). The quality and quantity of total RNA were assessed by using NanoDrop ND-1000 (Thermo Fisher Scientific, MA, USA). The RNA integrity was measured using denaturing Agarose gel electrophoresis. Seq-Star T M poly(A) mRNA Isolation Kit (Arraystar, MD, USA) was used to depurate mRNA from total RNA. he GenSeq™ m6A-MeRIP Kit (GenSeq Inc., China) was used to perform m6A RNA immunoprecipitation by following the manufacturer's instructions. Both the input samples without immunoprecipitation and the m6A IP samples were used for RNA-seq library generation with NEBNext® Ultra II Directional RNA Library Prep Kit (New England Biolabs, Inc., USA).