GSM6571019: DKO_Flcn-Tfe3_8; Mus musculus; RNA-Seq
RNA was quantified and mixed at 10ng/µl. Total RNA (100 ng) from each sample was prepared using QuantSeq 3'mRNA-Seq Library prep kit (Lexogen, Vienna, Austria) according to manufacturer's instructions. The amplified fragmented cDNA of 300 bp in size were sequenced in single-end mode using the NovaSeq 6000 (Illumina) with a read length of 100 bp.