GSM7876460: Jurkat cells, DMSO, 6 hours, RUNX3 antibody [DMSO_RUNX3_rep2]; Homo sapiens; OTHER
Cells were fixed with 1% formaldehyde for 1 minute. After quenching and permeabilization, cells were treated with respective antibodies Samples were treated with pA/G-Mnase to cleave target DNA sequences following antibody incubation CUT&RUN libraries were prepared using NEBNext DNA Ultra II  Library Prep kit according to manufacturer instructions. Libraries were amplified for 15 cycles in the thermo cycler and samples purified with magnetic purification beads (NEB  E7104S)