GSM6912729: sg_trcSL1c5_rep1; Escherichia coli; RNA-Seq
total RNA was extracted using RNeasy Plus Mini Kit (Qiagen) with on-column DNase treatment using RNase-Free DNase Set (Qiagen). rRNA was depleted with Ribo-Zero plus rRNA Depletion Kit (Illumina) and cDNA library was constructed using KAPA Stranded RNA-Seq Kits (Kapa Biosystems).