GSM8008506: Epidermis, Cers4epi-/-, rep 1; Mus musculus; RNA-Seq
Back skin of. control and Cers4 epi -/-  P19 female mice were dissected and single cell suspensions were generated using a mixture of DNAse I (40 µg/ml), Liberase (0.125mg/ml; Roche Cat.NO. 05401119001 and Papain (30 U/ml Worthington Cat. NO. LK003150) digestion in S-MEM (Gibco) for 60 min at 37 °C. Cells were labeled with Mouse Immune Single-Cell Multiplexing Kit (BD) according to manufacturers instructions. Single cell whole transcriptome analysis libraries were prepared according to the manufacturer's instructions using BD Rhapsody WTA Reagent kit (BD, 633802) and sequenced on the Illumina NextSeq 500 using High Output Kit v2.5 (150 cycles, Illumina) for 2 × 75 bp paired-end reads with 8 bp single index aiming at sequencing depth of >20,000 reads per cell for each sample.