Alex_Yellow_Chicago_lib
Long-ranged mate pairs were produced using the Chicago method and sequenced by Dovetail Genomics. High-molecular-weight (HMW) genomic DNA for Chicago libraries was extracted from a single Alex Yellow female, and and proximity ligated as described previously (Putnam et al., 2016). After ligation, crosslinks were reversed and the DNA purified from protein. The DNA was sheared to ~350 bp mean fragment size, and sequencing libraries were generated using NEBNext Ultra enzymes (New England Biolabs, Ipswich, USA) and Illumina-compatible adapters. The libraries were sequenced on an Illumina HiSeqX to produce 330M read pairs.