Paired-end RNA-seq of larval Aethina tumida from Ohio
RNA was extracted using the Direct-zol RNA Miniprep Plus kit with DNase treatment. Ribodepletion was performed using the Illumina Ribo-Zero Plus kit (no polyA purification). NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (strand-specific) was used for library preparation. Ribodepletion, library preparation, and sequencing were performed at the Interdisciplinary Center for Biotechnology Research (ICBR) at the University of Florida