Whole exome sequencing of a PHTS-associated thyroid tumor
Tumor-normal pairs were sent to the Broad Institute Genomics Services for somatic exome sequencing (Cambridge, MA, USA). There they performed library generation and exome sequencing using an Illumina NovaSeq6000 platform according to their standard pipeline with a target depth of 100x. Output sequencing reads were quality controlled and aligned by the Broad Institute using their standard GATK pipeline, aligning to the reference genome GRCh37/hg19.