GSM8240673: Rec_3; Mus musculus; RNA-Seq
RNA was extracted from cell lines using Promega ReliaPrep™ RNA Cell Miniprep System(Cat#Z6011) We performed a "Illumina stranded mRNA ligation" (ISML) prep starting from 250ng of RNA, according to Illumina protocol 1000000124518 v03. We quantified the libraries by qubit DNA HS and performed profile analysis by TapeStation TS4200. We sequenced the libraries on Illumina NovaSeq 6000 in a PE60 configuration. We trimmed reads for ISML (Nextera) adapters using bclconvert (v 00.000.000.3.9.3).