GSM8185883: eihsc, NF1 deficient; SUZ12 deficient, origin 32.4, ChiP H3K27ac; Homo sapiens; ChIP-Seq
Lysed cells were treated with 100 gel units of MNase in MNase digestion buffer (20 min, 37°C, continuous thermal mixing). Following sonication in Diagenode Bioruptor pico (Denville, NJ), supernatant was subjected to ChIP with antibody overnight Chip and library preparation were performed following the description of Caride et al. (2023) BMC Genomic. Titration-based normalization of antibody amount improves consistency of ChIP-seq experiments