Apo22_M4
Aposymbiotic Riptortus pedestris insects were harvested in the second instar at 2 day. Insects were dissected and the M4 midgut region were harvested in RNAiso Plus (Takara Bio). RNA was extracted with the RNeasy mini kit (Qiagen). mRNA purification and fragmentation and the preparation of cDNA libraries were conducted using TruSeq RNA Library Prep Kit v2 kit (Illumina). The cDNA libraries were sequenced by Illumina HiSeq-2000.