GSM7846474: Capture Hi-C in wild type mouse bone marrow derived macrophages treated with 100 uM heme (rep1); Mus musculus; OTHER
Mouse bone marrow-derived macrophages were cross-linked using formaldehyde-based cross-linking method and processed to Hi-C using the Arima Hi-C kit. A capture enrichment step was applied to the generated Hi-C libraries using sustom-designed probes (SureSelect, Agilent Tier 2 -2,9Mb ). Our design has two components combined in the same probe set : a. The Hmox1 genomic locus (tiled probescovering a 500kb area). The coordinates of this area arechr8:74850001-75350000. b.9127 sites covering the top BACH1's ChIP-seqsites (in untreated BMDMs) sorted based on the RPKM. Hi-C and cHi-C libraries were prepared with the Arima Hi-C kit and Agilent SureSelect library systems according to the manufacturer protocol.