Labilibaculum sp. K2S
Genomic DNA was extracted using a Quick DNA Fungal/Bacterial Kit (Zymo Research, Orange, CA, USA). The quality of the extracted DNA was assessed based on the A260/280 ratio using a Nanodrop 8000 (Thermo Fisher Scientific, Waltham, MA, USA) and results of DNA electrophoresis in a 1% agarose gel. DNA concentration was quantified using a Qubit 4 Fluorometer and a dsDNA High Sensitivity Assay Kit (Life Technologies, Carlsbad, CA, USA). The DNA library for whole-genome sequencing was prepared using a NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA, USA). The library was validated using capillary electrophoresis on a QIAxcel Advanced System (Qiagen, Hilden, Germany) using the QIAxcel DNA High Resolution Kit and normalized using qPCR on the CFX Connect Real-Time PCR System (Bio Rad, Hercules, CA, USA). Paired-end sequencing (2 250 bp) was carried out on a MiSeq platform (Illumina, San Diego, CA, USA) using Reagent Kit v.2 (Illumina, San Diego, CA, USA). DNA extraction, DNA library preparation and its whole-genome sequencing were conducted in the Science Recourse Center Persistence of microorganisms of the Institute for Cellular and Intracellular Symbiosis UrB RAS.