GSM7502319: H3K9me3_ChIP_E165_het_rep2; Mus musculus; ChIP-Seq
Germ cells were fixed with 1% FA. Chromatin fraction was extracted after processing with sonication. Chromatin extract was served for immunoprecipitation with H3K9me3 antibody. DNA was purified from co-precipitaed fraction. ChIP-seq library was prepared using QIAseq ultralow input library kit (QIAGEN), following manufacturer's instruction.