GSM8417204: AJ_WGD+_aPDL1_2; Mus musculus; RNA-Seq
Total RNA were extracted following manufacturer's protocol pf Qiagen Rneasy Mini Kit. (Qiagen, #74106) RNA-seq libraries were prepared using Illumina TruSeq Stranded mRNA sample preparation kits from 500 ng of purified total RNA according to the manufacturer's protocol. The finished dsDNA libraries were quantified by Qubit fluorometer, Agilent TapeStation 2200, and RT-qPCR using the Kapa Biosystems library quantification kit according to the manufacturer's protocols.