GSM7502681: BHT101; Homo sapiens; RNA-Seq
Total RNA was harvested using the RNeasy Mini Kit according to the manufacturer's protocol. RNA quality was checked using a Fragment Analyzer 5200 cDNA libraries were prepared from 100 ng of total RNA using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New Englands Biolabs) following manufacturer's recommendations. Paired-end sequencing was performed with a NovaSeq 6000 (Illumina).