DNAseq of Acinetobacter baumannii
Extracted gDNA was treated with the NEBNext Ultra II End Repair/dA-Tailing Module (NEB). Then barcodes from the Native Barcoding Expansion 1-12 & 13-24 from Oxford Nanopore Technologies (ONT) were ligated using the NEBNext Ultra II Ligation Module (NEB). DNA was purified using Ampure XP beads (Beckman Coulter). The DNA from different barcoded samples was pooled and the adapter AMII (ONT) was ligated using the NEBNext Ultra II Ligation Module (NEB). DNA was purified using Ampure XP beads (Beckman Coulter), followed by sequencing with a R10.4 MinION Flow Cell using a MinIon Mk1B (ONT).