GSM6833477: gRNA IL2validation popIL2 rep3; Homo sapiens; OTHER
Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Genomic DNA (gDNA) was extracted from sorted cells for different cytokine populations using Quick-DNA Miniprep kit (Zymo) or a custom precipitation-based gDNA extraction protocol. gRNA enrichment libraries were prepared from gDNA using a 3-step custom PCR protocol using gRNA-specific nested primers.