Doubled hapolid A4-3 genome sequence
Libraries were prepared using the Illumina TruSeq Nano DNA Library Preparation Kit following manufacturer's recommendations. Completed libraries were QC<92>d and quantified using a combination of Qubit dsDNA HS and Agilent 4200 TapeStation HS DNA1000 assays. The libraries were pooled in equimolar quantities for multiplexed sequencing and the pool quantified using the Kapa Biosystems Illumina Library Quantification qPCR kit. This pool was loaded onto one (1) lane of an Illumina HiSeq 4000 flow cell and sequencing was performed in a 2x150bp paired end format using HiSeq 4000 SBS reagents.