Defining focal neuroendocrine differentiation as a transcriptionally distinct form of prostate cancer pathology characterized by the expression of androgen receptors.
scRNA-Seq was done on dissociated PDXs using the 10X Genomics Chromium Single Cell 3′ Library & Gel bead Kit V3.0, per the manufacturer's instructions (CG000183 Rev C). Briefly, 5000 PDX cells were utilised per sample as input. By encapsulating cells in microfluidic droplets, around 4000 single-cell transcriptomes were recovered per sample.