GSM7502153: α-synuclein 0 mg/mL pull-down by anti-α-synuclein antibody; synthetic RNA; OTHER
Bound RNAs were extracted from the protein by phenol/chloroform isolation and ethanol precipitation. RNAs were reverse-transcribed into cDNA using Superscript III (Invitrogen) according to manufac-turer's instructions together with the following primer: 5'-GCCTTGGCACCCGAGAATTCCA-3'. To control for any nucleotide biases in the input random library, the input RNA pool (0.5 pmol) was also reverse-transcribed and Illumina sequencing library prep was followed by 8–10 cycles of PCR using High Fidelity Phusion (New England Biolabs). All libraries were barcoded in the PCR step, pooled together,