GSM7720997: NTN_3M; Mus musculus; RNA-Seq
After digesting kidneys with 0.2 mg/ml liberase (Roche) and 4 mg/ml DNase I at 37°C for 25 minutes in a shaking incubator, cells were filtered through a 70-μm and 40-μm strainer. Isolated cells were re-suspended in DMEM consist of 10% FBS. Libraries were prepared using the chromium controller according to the 10× Chromium Next GEM Single Cell 3′ protocol (version 3.1; 10× Genomics).