RNAseq of rapeseed root :ZS9
After the RNA samples were tested, eukaryotic mRNA was enriched with magnetic beads with Oligo(dT) (in case of prokaryotes, mRNA was enriched by removing rRNA through the kit) . Subsequently, mRNA was interrupted into short fragments by adding fragmentation buffer. One-stranded cDNA was synthesized using six-base random primers (random hexamers) with mRNA as template, and then two-stranded cDNA was synthesized by adding buffer, dNTPs, and DNA polymerase I and RNase H. Subsequently, two-stranded cDNA was synthesized by utilizing the Double-stranded cDNA was purified using AMPure XP beads. purified double-stranded cDNA was first subjected to end repair, addition of A-tail and ligation of sequencing junctions, followed by fragment size selection using AMPure XP beads. Finally, PCR amplification was performed and the PCR products were purified by AMPure XP beads to obtain the final library.