GSM8187176: MCF7  cells, LY2603618 treated; Homo sapiens; OTHER
Genomic DNA was extracted using DNesy kit (Qiagen).  1μg of total DNA was used for library preparation. PCR amplification of shRNA, and PCR product purification were performed according to the manufacture's (RHS6083) protocol. Purified amplicons from different samples were pooled and analyzed with a Bioanalyzer (Agilent).