GSM8186787: EAT 2,VDJ, TCR; Homo sapiens; OTHER
Two paired human epicardial adipose tissue and right atrial appendage tissue specimens were obtained during open chest cardiac surgery. The tissue specimens were enzymatically digested to isolate the stromal vascular fraction. The CD45+ immune fraction was then isolated through PE bead enrichment using Nanobeads.  Cells were stained with CITE-Seq antibodies (antibody-derived tags) and further CD45+ enrichment was performed using fluorescence-activated cell sorting (FACS). The samples were maintained at a concentration of ~1000 cells/μL. Single cells were encapsulated in Gel Beds-in emulsion (GEMs) using the 10x Genomics Controller with a Chromium Single Cell 5' GEM Library and Gel Bead Kit. The CITE-seq protocol described in the 10x Genomics document CG000330 - Chromium Next GEM Single Cell 5' Reagent Kits v2 (Dual Index) User Guide with Feature Barcode technology for Cell Surface Protein & Immune Receptor Mapping was followed to generate, a 5' gene expression library, cell surface protein library (antibody-derived tags) and VDJ libraries for TCR sequencing.