GSM7720238: 3T3_CBX7_C3_rep1; Mus musculus; ChIP-Seq
CBX7 ChIP-seq for WT and CBX7-mCherry cell lines were performed as previously described with modifications (Y. Zhang et al., 2019.). Cells were centrifuged and resuspended in 1 × PBS containing 1 % formaldehyde (Sigma) for 10 min at room temperature for fixation. Glycine was then added to a final concentration of 0.14 M and incubated for 10 min at room temperature to quench fixation. Crude nuclei were purified and the lysate is sonicated using the program configured as power 10, cycle 12, 15 seconds sonication, and 45 seconds rest for every minute. For each ChIP assay, proteinA/G beads bond antibodies (3ug, ab21873) were added and incubated at 4◦C overnight. After washing, Chromatin was then reverse-crosslinked and purified by 2.5 × DNA beads (Vazyme). Tru-seq library preparation was done using NEBNext® Ultra TM II DNA Library Prep Kit for Illumina® v1.0. The amplified DNA was size selected using SPRI beads.