RNA-Seq of Symphysodon sp.: red melon adult cutaneous sample
The total RNA of the skin specimen was extracted by TRIzol Reagent (Thermo Fisher) according to the manufacturers protocol. The extracted mRNA was enriched by using the bead-based NEBNext Poly(A) mRNA magnetic isolation module (NEB) and the RNA library was prepared by using NEBNext Ultra II RNA Library Prep Kit (NEB), in accordance of manufacturers protocols. The mRNA was fragmented at high heat in the presence of divalent metal ions followed by cDNA synthesis and second strand synthesis. The double stranded DNA was then end-repaired and ligated with Illumina adaptor, followed by index PCR to incorporate sample-specific 8-bp dual barcode.