GSM7498566: WT-M25; Musculus; RNA-Seq
Total RNA was extracted from sWAT using an RNeasy Plus Micro Kit (Qiagen). RNA samples were quantified using a Qubit 4 fluorometer (Thermo Fisher Scientific), and RNA integrity was determined using a 4200 TapeStation System (Agilent Technologies). Libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England BioLabs) in accordance with the manufacturer's instructions.