GSM8395976: FLO-1, GFP_1; Homo sapiens; RNA-Seq
RNA was extracted using Monarch® Total RNA Miniprep Kit. The samples were prepared according to the MGIEasy RNA Directional library preparation guide with the Dynabeads® mRNA purification kit for mRNA enrichment The libraries that passed QC were pooled based on library concentration and target data volume for sequencing on the MGI platform, DNBSEQ-G400 set at 100 million total raw reads. The mRNA sample is enrichment was performed with Dynabeads® mRNA Purification Kit before being subjected to RNA fragmentation. The fragmented mRNA is reverse transcribed to cDNA and is followed by a second strand synthesis, end-repair and A-tailing, adapter ligation, and PCR amplification .