Illumina paired end sequencing
Leiomyosarcoma
cDNA libraries was constructed by Rocha KAPA mRNA HyperPrep Kit (KR1352-v5.17) using 1000 ng RNA, following manufacture's protocol. The adaptor-ligated libraries were enriched by 10 cycles of polymerase chain reaction (PCR). Libraries were sequenced using Illumina NovaSeq6000 (2 x 151 bp). Fusion events were identified using STAR-Fusion (version 1.1.0) with the following default parameters: --FusionInspector inspect --annotate --examine_coding_effect --extract_fusion_reads. Key genes involved in DNA repair and PLK4 analysed in this study was extracted in the final BAM files.