GSM7719890: Urinary exosome, ultra-high speed centrifugation [C7]; Homo sapiens; OTHER
Urine was mixed with RiboTM Exosome Isolation Reagent and exosome isolation was performed according to the manufacturer's instructions (Ribobio, China). Exosomal RNA was extracted by HiPure Liquid miRNA Kit/HiPure Serum/Plasma miRNA Kit (Megan, China). Exosomal RNA were used to prepare small RNA libraries by NEBNext® Multiplex Small RNA Library Prep Set for Illumina (NEB,USA) according to manufacturer's instructions