COI metabarcoding of Caranx crysos
PCRs were carried out in 25 l volumes with 1X Q5 High-Fidelity master mix (New England Biolabs), 1X Q5 enhancer (New England Biolabs), 0.5 M of each primer and 2-3 ng of template DNA (including unique combinations of dual-indexing barcodes). The annealing temperature was 55C and the thermal profile was as recommended by the supplier.