GSM8239689: KA002: MIAPACA2, PATU8902, SW1990; Homo sapiens; RNA-Seq
Dissociated cells were washed twice with PBS 0.1% BSA buffer with cold centrifuging at 500rpm and 5'. Finally, cells were stained with 0.4% Trypan blue (Invitrogen, Cat. No. T10282) to check the viability and diluted with PBS 0.1% BSA to about 1E106 cells/ml for single cell sequencing. For each single cell run, 6 samples were combined Dissociated cells were processed for single-cell gene expression capture (scRNA-seq) using the 10X Chromium 3' Library and Gel Bead Kit (10x Genomics), following the manufacturer's user guide. Libraries were prepared following the manufacturer's user guide and sequenced at the Columbia University Single Cell Analysis Core