GSM6570979: Astro_DIV14_IRKO_IGF1 rep1; Mus musculus; RNA-Seq
Total RNA was extracted from primary cultured control and IRKO astrocytes treated with 100 nM insulin, IGF-1 or vehicle using QIAzol reagent (Qiagen). RNA quality and quantification were verified using a 2100 Agilent Bioanalyzer. Total RNA samples (2 μg, RNA integrity score > 7) were submitted for library preparation and next-generation sequencing by Novogen. Briefly, mRNAs were purified from total RNAs using poly-T oligo-conjugated magnetic beads. Non-stranded cDNA libraries were prepared and subjected to sequencing (paired-end 150bp).