GSM6734627: Ishikawa_NC; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a Bioruptor automatic sonicator (Diagenode, Denville, NJ). Antibody immunoprecipitation DNA was quantified with QubitTM (Thermo Fisher Scientifc,USA) and Agilent 2200 TapeStation (Agilent Technologies, USA). The DNA library building kit from NEB corporation was used to construct the sequencing library.