GSM6697715: PSA4 VDJ; Homo sapiens; RNA-Seq
Synovial fluid mononuclear cells (SFMC) were isolated from four patients with psoriatic arthritis by density gradient centrifugation using Lymphoprep (Axis-Shield, Oslo, Norway). Cells were cryopreserved and stored in liquid nitrogen in culture medium containing 90% FCS and 10% DMSO (Fisher Scientific, Loughborough, UK). After thawing, 2 million SFMCs were labelled with fluorescently labelled antibodies for flow sorting as well as CITE-Seq (Cellular Indexing of Transcriptomes and Epitopes by sequencing) antibodies.  CD8+ T-cells were sorted on a FACSAria/LSRII. 20,000 cells per patient were loaded for gene expression and immune profiling using the 10X Genomics Chromium Single Cell platform. Library was performed according to the manufacter's instructions (single cell 5' v2 protocol with feature barcoding and VDJ sequencing, 10x Genomics).