GSM6569709: A549_3; Homo sapiens; RNA-Seq
Total RNA of fibroblasts, LUAD cells, and educated LUAD cells was  extracted with TRIzol. Exosome-packaged RNA extraction was performed using Total Exosome  RNA and Protein Isolation Kit (4478545, Invitrogen). For RNA sequencing, libraries were prepared  from purified RNA using a NEBNext Ultra Directional RNA Library Prep Kit to ensure the RNA was not fragmented prior to library preparation.