GSM7846881: IkBz_1.0h_R2; Mus musculus; ChIP-Seq
Samples were prepared as previously described (Barish et al., 2010). Crosslinking conditions of 1.0mM DSG and 1.0% formaldehyde were used for p50 and RelA ChIP-seq. Crosslinking conditions of 3.0mM DSG and 1.0% formaldehyde were used for IkBz ChIP-seq. Chromatin shearing was done using either the Bioruptor or Covaris M220. Immunoprecipitation was performed with either anti-RelA, anti-IkBz, or anti-p50 antibodies. Using the Kapa LTP Library Preparation Kit, libraries were constructed according to manufacturers instructions.