Partially edited A6 and COIII PF T. brucei brucei in RESC8 RNAi uninduced and induced
Cells were grown with or without tetracycline for two days and RNA was extracted. cDNA was generated using the 3' primer specific to each transcript and libraries were generated for each transcript, maintaining amplification within the linear range of the PCR machine. Whole combinations of pre, fully, and partially edited mRNAs were sequenced using paired-end Illumina MiSeq.