GSM7720553: Msh3+/- Striatum female snRNA rep1 [Msh3 snRNA-seq]; Mus musculus; RNA-Seq
Brain striatal tissues were dissected and snap-frozen on dry ice. Nuclei were isolated using the 'Frankenstein' protocol by Luciano Martelotto (10x Genomics Customer Developed Protocol). With FACS, 16,500 DAPI+ nuclei were collected for each sample and loaded onto the 10X chromium device. The library preparation and sequencing were performed following 10X Genomics Chromium protocols.