GSM6398641: neuroblastoma SK-N-AS xenograft, brequinar treated 72h, rep2; Homo sapiens; RNA-Seq
Mouse tumor tissue used for RNA sequencing was stored in RNAlater solution (Thermo Fisher, Waltham, MA, USA) at -80 °C. Total RNA from xenograft/tumor tissue was isolated using the RNeasy Mini Kit (Qiagen). All libraries were generated using the TruSeq Stranded protocol (Illumina). All libraries were sequenced on the NextSeq500 platform. Xenograft samples were sequenced with a 75 bp single-end strategy. TH-MYCN samples were sequenced with a 2x42 bp paired-end strategy.