GSM6568571: REST_PAN_pd_rep_2; Mus musculus; RNA-Seq
RNA from inputs and streptavidin pulldowns was extracted using the TRIzol protocol. RNA (input and pulldown, ~100 ng) was used as the starting material and first treated with DNAse on columns. TruSeq stranded RNA sample protocol was followed using RiboZero to get rid of ribosomal RNAs. For unique adapters, IDT Illumina Truseq RNA UD Indexes were used. The samples were then pooled and sequenced on the Illumina NovaSeq 6000 sequencer.