Potter Cove sediment slurry incubation day 0 - Bacteria 16S rRNA gene
Combined DNA and RNA were extracted from slurry with a phenol-chloroform protocol. For RNA samples cDNA was synthesized. PCRs were performed using primers with unique 8 bp barcodes at forward and reverse primers. Multiple samples were pooled into one library for sequencing. During demultiplexing reads were sorted by read direction forward-reverse or reverse-forward using the primer sequence, resulting in multiple paired fastq files (=runs) for the same ENA experiment