GSM7727894: Tumor-derived cell line from 690108 (TOPCDS); Mus musculus; RNA-Seq
RNA was extracted and purified from flash frozen tumors/normal tissues and cells using a Qiagen RNeasy kit following the manufacturer's instructions for fibrous tissue (Qiagen, Hilden, Germany). High quality RNA (RIN >7) was divided into triplicate and used to make 150bp paired-end, rRNA-depleted, libraries using the Illumina TruSeq RNA library Prep Kit (Illumina, CA, USA).