GSM7286716: F6-ATAC; Gallus gallus; ATAC-seq
ATAC experiments were performed as described in Buenrostro, J. D. et al. 2013. Briefly, 1 x 105 asynchronous, G1 and G2/M cells collected by elutriation were washed once with cold PBS. The pellet was resuspended in 50μl of 20 Lysis buffer (0.3M Sucrose, 10mM Tris pH8, 10mM NaCl, 3mM MgCl2, 0.2% Triton X100) and the transposition reaction was incubated for 30 min at 37°C in a thermomixer shaking at 700rpm. Samples were purified with the MinElute PCR purification kit (Qiagen #28004) following manufacturer instructions and eluted in 11μl. The tagmentation and library preparation was performed with the Nextera DNA Library Preparation Kit with 8 cycles of PCR amplification (Illumina #FC-121-1030) and the libraries were cleaned up with SPRselect beads (Beckman Coulter, #B23317).