GSM7498285: BMDM, Irf3, stimulated 120', r2; Mus musculus; ChIP-Seq
BMDMs were fixed in 1% formaldehyde for 10 min and quenched with 500mM Tris pH8.0 for 15 min. After cell lysis, chromatin was sheared with Covaris M220 sonicator. Sonicate lysate were incubated with Rel-A (Santa Cruz #sc-372), IRF-3 (Santa Cruz # sc-9082 X) and Brg1 (Cell Signaling # 9284S) overnight at 4oC. The DNA/protein-antibody complexes were captured by Dynabeads protein A/G. DNA fragments were extracted and prified by phenol-chloroform and ethanol precipitation. Libraries were prepared with Kapa Hyper Prep Kit Illumina Platform (#KK8504) according to the manufacturer's instructions.