RNA-Seq of Xenopus tropicalis liver NF54 treated with CORT
Tissues were lysed in 500 l of RNAble (Eurobio) using the Tissue Lyser II apparatus (QIAGEN), and 100 L of chloroform. RNAs were purified from supernatant with the RNeasy MinElute Cleanup kit (ref: 74204, QIAGEN) according to the manufacturer's instructions. Libraries were prepared from mRNA purifierd from 1g of total RNA, using the strand non-specific RNA-Seq library preparation TruSeq RNA Sample Prep v2 kit (Illumina).