GSM8284058: HiC control crispr; Homo sapiens; Hi-C
HiC in nuclei were performed with 2x106 cells. The cells were fixed with formaldehyde 2%. Chromatin was digested with DpnII enzyme and incubating overnight. The 5′ overhangs were filled with dCTP, dGTP, dTTP and Biotin-14-dATP and re-ligated in nuclei. DNA fragments were purified and sonicated in COVARIS S220. The DNA were selected using Ampure SPRI Beads to get 300-600bp fragments. The pull-down was performed with Dynabeads MyOne Streptavidine C1. The libraries were marked with Illumina TrueSeq DNA Adapters and amplified using Taq Phusion NEB. The paired-end sequencing was performed in Illumina NovaSeq 6000.