ChIP-seq of strawberry fruit shelflife
Fragaria ananassa cv. Elsanta strawberry fruit at red ripe stage were washed with water, the calyx was removed using a sharp blade, and they were immersed in 200 ppm sodium hypochlorite for 2 min. They were then air dried in a laminar air flow hood and halved. Halved fruit were stored at 8C and sampled for ChIP and RNA extraction at 0 and 5 days of storage. RNA was extracted according to Greco et al. (2014) https://doi.org/10.1371/journal.pone.0096470. Immunoprecipitated DNA concentration was checked using a Qubit Fluorometer and DNA quality via a High Sensitivity D1000 ScreenTape (Agilent, #5067-5584). A NEXTflex Rapid DNA-Seq Kit (PerkinElmer # NOVA-5188) was used for paired-end DNA library construction. Sequencing used an Illumina Next Seq 5000 Platform (120M reads per run) performed by the Biosciences Genomics Research Hub within the School of Biosciences, Cardiff University.