GSM6943600: gd3_B; Mus musculus; RNA-Seq
A single-cell suspension of skin cells was generated from 30 pooled WT mice. Dermal gd T cells, DETCs, and GD3 cells were FACS sorted into 50% fully-supplemented RPMI and 50% FBS. After washing the sorted cells in FACS Buffer, each population was loaded as a separate channel on the Chromium 10x Genomic v1.1 platform (10X Genomics). Libraries were prepared using the Chromium Single Cell 5' Library Construction Kit and generated from the cDNA produced through the Chromium chemistry. Nucleic acid products were separated using SPRIselect beads (Beckman Coulter). Libraries were quantified using the Bioanalyer High Sensitivity DNA analysis kit (Agilent). Sequencing of GEX libraries was performed using the Nextseq 500/550 High Output kit v2.5 (75 cycles; Illumina) and sequencing was performed on the NextSeq 550 (Illumina).