GSM7720291: Monocytes subsets,donor 1, scRNA; Homo sapiens; RNA-Seq
The different cell populations (CL, INT, NC, slan neg/NC and slan pos/NC) were sorted from peripheral blood by using a Becton Dickinson FACS Aria Fusion (BD Biosciences) and then labelled with the BD Single-Cell Multiplexing Kit (BD Biosciences), strictly following the manufacturer's protocol. Both cell viability and counts were then evaluated, and only samples with high viability (> 85 %) were used for sequencing. Approximately 15000 cells were pooled together and loaded in a single BD Rhapsody cartridge. The procedure described above was repeated for two different donors cDNA Libraries were prepared using the BD Rhapsody Whole Transcriptome Analysis (WTA) Amplification kit and BD Single-Cell Multiplexing kit (BD Biosciences) following the manufacturer's protocol