GSM6433245: BC2; Mus musculus; RNA-Seq
Total RNA was isolated from three random frozen hippocampus samples from the same strain, using the TRIzol reagent (Invitrogen, Carlsbad, CA, USA) Next generation sequencing library preparations were constructed according to the manufacturer's protocol (NEBNext® UltraTM RNA Library Prep Kit for Illumina®). Then libraries with different indices were multiplexed and loaded on an Illumina HiSeq instrument according to manufacturer's instructions (Illumina, San Diego, CA, USA).