Illumina HiSeq 2500 paired end sequencing; Single-cell RNA-seq (10x Chromium) of FACS-sorted drl:H2B-Dendra2, etv2:Kaede, fli1:DsRed, and tp1:eGFP transgenic zebrafish embryos
Single-cell RNA-seq (10x Chromium) of FACS-sorted drl:H2B-Dendra2, etv2:Kaede, fli1:DsRed, and tp1:eGFP transgenic zebrafish embryos
Dechorionated embryos were collected at defined stages, dissociated by pipetting, centrifugation at 300g for 5 minutes, washed once with PBS, and sorted by FACS. Total cell concentration and viability were determined using a TC20 Automated Cell Counter (Bio-Rad). Samples were then re-suspended in 1x PBS with 0.05% BSA at 800-3000 cells per ml. Samples were loaded on the 10x Chromium system with a target of 4,000 cells. Chromium Single Cell 3สน GEM, Library & Gel Bead Kit v2 or v3 was used for library preparation. Samples were loaded on the 10x Chromium system with a target of 4,000 cells. Chromium Single Cell 3สน GEM, Library & Gel Bead Kit v2 or v3 was used for library preparation.