ALKALI-BUFFERING EFFECT TO INCREASE HYDROGEN PRODUCTION UNDER DIFFERENT SUBSTRATE CONCENTRATIONS ON CO-FERMENTATION OF CITRUS AGROINDUSTRIAL WASTES
Genomic DNA was extracted with FastDNA SPIN Kit for Soil DNA Extraction (MP Biomedicals) following the manufacturer's recommendations. The integrity of the extracted DNA was checked on a 0.8% agarose gel and the quantification (ng/L) and purity (260/280nm ratio) was performed on the Nanodrop 2000 Spectrophotometers (ThermoFisher Scientific). The sequencing of the 16S rRNA gene (V3+V4 region) was performed by the company NGS genomic solutions (Piracicaba, SP - Brazil), and basic bioinformatics analysis for taxonomic identification. Amplification of the 16S rRNA gene was performed with a set of 341F/806R primers (Klindworth et al. (2013); Caporaso et al (2011), and purified with AMPure XP beads. The Nextera XT kit was used to include the barcodes. Sequencing libraries were generated using the DNA library UltraNBNext Kit for Illumina (New England Biolabs, Ipswich, USA), following the manufacturer's recommendations.