soil31
DNeasy PowerSoil Pro Kit (Qiagen, Germany) was used to extract genomic DNA from the soil samples according to the manufacturer's protocol. DNA quality and quantity were measured using a NanoDrop UV-Vis spectrophotometer and a Qubit fluorometer 2.0 (Thermo Fisher Scientific, USA). For the fungal community, the ITS2 region of the ITS nuclear region was amplified using ITS86F (5-ACA CTC TTT CCC TAC ACG ACG CTC TTC CGA TCT GTG AAT CAT CGA ATC TTT GAA-3) and ITS4 (5-GTG ACT GGA GTT CAG ACG TGT GCT CTT CCG ATC TCC TCC GCT TAT TGA TAT GC-3) primers. Library quality was evaluated using an Agilent 2100 Bioanalyzer (Agilent Technology, USA). Lastly, sequencing was carried out at the next-generation sequencing (NGS) core facility of Kyungpook National University (Daegu, South Korea) and using an Illumina MiSeq platform (Illumina, USA) with single-end 250 bp reads.