GSM7933080: Healthy control [H_1]; Homo sapiens; miRNA-Seq
Total RNA and Exosomal RNA were isolated using Trizol reagent. The RNA concentration and purity were confifirmed using absorbance measurements with a NanoDrop 2000 (Thermo Scientifific, USA). 1 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for miRNA-seq were prepared using NEBNext® Multiplex Small RNA Library Prep Set for Illumina