GSM8187229: C_3, Control; Homo sapiens; RNA-Seq
Total RNA was extracted using the magnetic bead method (Yuan, Yu-BR02-1, China). 2 µg of total RNA was used for the construction of sequencing libraries. The rRNA was removed using a Ribo-zero reagent kit. The synthesized double-stranded cDNA fragments were screened and purified several times by VAHTSTM DNA Clean Beads.