GSM6460010: CD4 T cells, Nacl 5mM (CTRL), rep3; Mus musculus; RNA-Seq
Cells pellets were collected for isolation of RNA with RNeasy mini kit from Qiagen (cat# 74104). RNA quality (RIN > 7) was confirmed using the TapeStation 4200 system (Agilent Technologies). Preparation of RNA library and transcriptome sequencing was conducted by Novogene Co., LTD (Sacramento, CA).