DNA sequencing of the RP1 plasmid
The RP1 plasmid was extracted from 20 ml of the overnight culture of P. aeruginosa PAO1/RP1, respectively, using the Plasmid Mini AX kit (A&A Biotechnology) according to the manufacturers instructions. The DNA library was prepared using the Nextera XT DNA Library Preparation Kit, indexed with the Nextera XT Index Kit v2 Set A and sequenced on the Illumina 2250-base paired-end v2 MiSeq platform.