RNA-Seq of healthy controls
cDNA libraries are constructed using a Nextera XT DNA Library Preparation kit (Illumina). Tagmentation is performed first to fragment the cDNA and add adapter sequences to the end of each fragment. After the tagmentation reaction is neutralized, libraries are PCR amplified with Illumina p7 and p5 index adapters. The cDNA libraries are then pooled and undergo a final 0.9X SPRI clean up. Library quality is assessed using a bioanalyzer trace, final libraries have a size of 500 bp.