GSM6734290: C9ORF72 ALS iMNs treated with SYF2 siRNA-2 replicate 1; Homo sapiens; RNA-Seq
RNA was extracted using QIAGEN RNeasy Kits according to the manufacturer's instructions. 3'-Digital Gene Expression RNAseq Library Kit (3'-DGE) 3' RNA-seq libraries were generated using the3'-Digital Gene Expression RNAseq Library Kit at Novogene Ltd, according to the manufacturers' directions. Libraries were sequenced at the Novogene on a Illumina NovaSeq 6000 sequencer. A total of 20 million to 30 million 150-bp, paired-end reads were obtained for samples.