Metagenomic DNA sequences of Bat Oral Swab (ID: OS_Apla_Pol_110)
Nucleic acid samples were used for library preparation and metagenomic sequencing using tagmentation which was performed according to the manufacturers recommendations (Illumina DNA Prep Kit, Illumina). Each library was marked with dual indexing using the IDT for Illumina DNA/RNA UD Indexes Set A and Set B (Illumina). After library preparation, the samples were submitted for Fragmentation Analysis to assess the quality of each library. This step was performed using the Fragment Analyzer and the NGS Fragment Kit (1-6000bp)(Agilent Technologies), aiming to quantitatively and qualitatively analyze NGS libraries from 100 to 6000 bp. The libraries that showed good fragment analysis quality were submitted for quantitative PCR with posterior pooling in accordance with each quantity and molecular weight. Sequencing was performed using Illumina NextSeq technology with 2x150bp and sequence depth of 20x.