GSM6833424: scRNA CRISPRa single perturbation S1; Homo sapiens; RNA-Seq
Cells were sorted by FACS for live cells and for the cell populations expressing the constructs of interest. Joint scRNA- and gRNA-seq libraries were prepared using the 10x Genomics Single Cell 5' Reagent Kit with Feature Barcode Technology. RNA libraries were constructed according to manufacturer's recommendations. For gRNA libraries, custom oligos complementary to the gRNAs were spiked into the reverse transcription reaction and gRNA library construction was performed using a custom PCR protocol following cDNA amplification.