Whole Genome Shotgun Sequencing of Aspergillus parasiticus
The genomic and mitochondrial DNA of Aspergillus parasiticus grown on malt extract agar was extracted using the NucleoSpin plant II kit (Macherey-Nagel). The quality and quantity of the DNA was checked using a NanoDrop 1000 (Thermo Fisher Scientific) and a fluorometer (Qubit 3.0, Thermo Fisher Scientific). The library was prepared using the Rapid Sequencing kit (Oxford Nanopore Technologies) and was sequenced using a MinION Mk1C instrument (Oxford Nanopore Technologies). Subsequent base calling was done using Guppy (Oxford Nanopore Technologies).