GSM7720781: SCaBER cells, GRHL3 clone 18; Homo sapiens; RNA-Seq
RNA isolation was performed using Nucleospin RNA Plus Kit (Macherey-Nagel), 1 µg of total RNA was used for mRNA Sequencing The quantity of RNA was analyzed with the NanoDrop 1000 (Thermo Scientific Nano Drop Technologies). RNA quality control was done with the Eukaryote Total RNA Nano Series assay using the Agilent 2100 Bioanalyzer system (Agilent Technologies, Inc., Santa Clara, USA). An RNA Integrity Number (RIN) of at least 8 verified the quality of the RNA. Illumina libraries were generated from 1 μg of total RNA using the NEBNext Ultra II Directional mRNA Library Prep Kit for Illumina as described by the manufacturer (New England Biolabs). The libraries were run on an Illumina NextSeq500 platform using the High Output v2.5 kit with 150 cycles (Illumina).