EVALUATION OF SIGNIFICANT FACTORS IN THE PRODUCTION OF HYDROGEN AND VOLATILE FATTY ACIDS  IN CO-FERMENTATION OF CITRUS PEEL WASTES AND PROCESSING WASTEWATER
Genomic DNA from the samples was extracted using the FastDNA SPIN Kit for Soil DNA Extraction (MP Biomedicals). The integrity of the extracted DNA was performed on a 0.8% agarose gel and the quantification (ng/L) and purity (260/280nm ratio) was performed on the Nanodrop 2000 Spectrophotometers (ThermoFisher Scientific). Then the DNA was delivered to the company GenomeDX  Gentica Avanada in Rio de Janeiro, RJ, for sequencing. The primer set used in the amplification of the 16S rRNA Genes (Region V3+V4) was the 341F - 806R (Youngseo et al., 2005).with the barcode. All PCR reactions were performed with Phusion High-Fidelity PCR Master Mix (New England Biolabs). Sequencing libraries were generated using NEBNext Ultra DNA Library Pre  Kit for Illumina, following the manufacturer's recommendations and index codes were added. Library quality was assessed on the Qubit @ 2.0 Fluorometer (Thermo Scientific) and Agilent Bioanalyzer 2100 system. Libraries were sequenced on the Illumina platform