GSM7412972: VisiumHuman_7_S7_L004; Homo sapiens; OTHER
For snRNA-seq, single nucleus suspensions were isolated from about 50mg frozen human cortex following Parse biosciences recommendation. For spatial transcriptomics, fresh frozen mouse and human brain samples were embedded in OCT medium and cryosectioned at 10um thickness and mounted on visium slides. snRNA-seq libraries were prepared using Parse biosciences Evercode WT kit (v1). For spatial transcriptomics, 10x genomics visium kit was used following manufacturer's protocol.