GSM6914211: TIR-STING, baseline- 0 hr; Bacteriophage sp.; OTHER
Plasmid DNA was recovered by miniprep, and library inserts amplified by PCR using primers flanking the ORF insertion site in pET-SUMO2. Bar-coded libraries for Illumina sequencing were generated using the sparQ DNA Library Kit (Quantabio) and 50 bp reads generated using a HiSeq3000 sequencing system.