WGS of Odocoileus hemionus
DNA samples were quantified using Qubit High Sensitivity Assay. 700 ng of DNA was used as input material for library preparation using the Illumina TruSeq PCR-free DNA Library Prep Kit following the manufacturers recommended protocol. In brief, DNA was fragmented to 400 bp on average using sonication on the Covaris LE220 instrument. Fragmented DNA was end-repaired, A-tailed and TruSeq Illumina dual-index adapters were ligated to the DNA. Libraries were validated on a Fragment Analyzer using the High Sensitivity NGS Kit to check for size and absence of primer dimers, and quantified by qPCR using Kapa Library Quantification Illumina/ABI Prism Kit protocol (KAPA Biosystems). Validated libraries were pooled in equimolar quantities and paired-end sequenced on the Illumina HiSeq X/Novaseq platform following Illuminas recommended protocol to generate paired-end reads of 150-bases in length.