GSM7413496: HNBC-1 - Serum - HNBC; Homo sapiens; miRNA-Seq
The pooled serum samples were centrifuged at 16000rcf in order to remove debris, platelets or cells, and RNA was extracted from 200 µl, using the Qiagen miRNeasy kit (Qiagen GmbH, Hilden, Germany), following the manufacturer's instructions. Syn-cel-miR-39 spike-in synthetic RNA (Qiagen GmbH, Hilden, Germany) was added to monitor extraction efficiency. RNA samples (6 µl for each pool) were used for library generation using the NEBNext Multiplex Small RNA Prep (New England Biolabs, Ipswich, Massachussets, USA), and sequenced on a single-read flow cell by bridge amplification and 75 cycles sequencing-by-synthesis on the Illumina NextSeq550 platform (Illumina Inc., San Diego, California, USA).