GSM8005850: SJ donor 10 and SJ donor 11 PBMC (SJ10_SJ11_PBMC) - GEX; Homo sapiens; RNA-Seq
PBMC were collected from each participant and frozen. CD45+ immune cells from all thawed PBMC samples were sorted via flow cytometric activated cell sorting. The 10x Genomics Chromium instrument was used to partition each single cell with barcoded beads. GEM generation and barcoding, cDNA amplification, cell surface protein and library construction was performed according to the 10X Genomics Chromium User Guide. Sequencing was performed using the Illumina NovaSeq 6000.