GSM6433223: MKN45, sensitive, rep2; Homo sapiens; RNA-Seq
RNA was harvested using Rneasy mini kit (Qiagen). 1 ug of total RNA was used for the construction of sequencing libraries. The RNASeq libraries were prepared using the Stranded Total RNA Prep with Ribo-Zero Plus (Illumina) following the manufacturer´s recommendations and the final library was validated on an Agilent 2100 Bioanalyzer with the DNA 7500 assay (Agilent).