GSM8188242: RMA-Qa1KO Tumor MHCIImin macrophages; Mus musculus; RNA-Seq
For single cell RNA sequencing (scRNAseq) mice bearing RMA-Qa1b-/- tumors were treated with therapeutic vaccination when tumors reached a size of 10-50mm3. At time of regression, tumors were harvested and processed into single cell suspensions. From single cell suspensions MHCII+ and MHCII- macrophages (Live, CD45+, CD11b+, F4/80+) were sorted using the BD Aria cell sorter and collected at a purity >90%. Single cell gene expression libraries were generated on the 10x Genomics Chromium X platform using the Chromium Next GEM Single Cell 3' Library & Gel Bead Kit v3.1 and Chromium Next GEM Chip G Single Cell Kit (10x Genomics) according to the manufacturer's protocol.