GSM6914639: early erythroid cells, EZH2-shRNA, day 7, Input; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a E220 focused-ultrasonicator (Covaris). ChIP-Seq libraries were constructed using Hiseq × Ten Reagent kit v2.5 (#FC-501-2501, Illumina) and then sequenced on an Illumina X-ten (E00487, Illumina).