18S V9 amplicon sequencing of sediments from the South Col of Mount Everest: SC2
The V9 region of the 16S ribosomal RNA gene was amplified using 1391-F/EukBR primers . Each primer combination had a unique barcode corresponding to a unique sample. Barcodeded amplicons were normalized and pooled into a single library which was sequenced on a 2x250 Illumina MiSeq run. Sequences were subsequently demultiplexed by barcode into individual files corresponding to each sample.