GSM6912073: P34_12wks_F_RNAseq; Homo sapiens; RNA-Seq
Frozen tissue was pulverized using a liquid nitrogen-chilled hammer mill and then homogenized in Trizol.  Following chloroform-mediated phase separation, the total RNA was purified from the aqueous phase using RNeasy columns (Qiagen, 74104) according to the manufacturer's protocol.  The RNA concentration was quantified using a NanoDrop and the quality was assessed by RNA ScreenTape (Agilent).  The total RNA was stored at -80°C until processed. Ten µg of total RNA were used as input for polyA RNA-seq library construction as previously described (Zhong et al., 2011)