Full 16S of Homarus americanus carapace: lesion carapace area of ISD moderately symptomatic animal
Carapace of Homarus americanus with or without impoundment shell disease (ISD) symptoms was rinsed twice with molecular grade water and material was collected with a swab. The DNA was extracted, amplified using Phusion Plus polymerase and universal primers 27F and 1492R. Amplicons were used to prepare libraries for sequencing.