GSM7500526: KRAS_DNA; Mesocricetus auratus; OTHER
Each sample collected ~ 1 million cells for RNA isolation using TRIzol™ Reagent (Thermo Fisher Scientific, #15596018). 3μg genomic RNA or 500 ng in vitro transcribed RNA was used for reverse transcription. The KRAS (S17N) DNA or  KRAS (S17N) cDNA mutant libraries were subjected to 28 cycles of PCR amplification using KRAS gene specific primers containing Illumina sequencing indexes (resulting in 330 bp amplicons). The PCR products were purified and used for sequencing on the Illumina platform.