GSM7877391: HUVEC_siRN20_60h_ATAC_r1; Homo sapiens; ATAC-seq
60h after transfection cells were counted and 50,000 cells were used for ATAC. Starting material: 50,000 cells; Tagmentation: Illumina Nextera Tagmentation kit; Adapters: Dual Index adapters from the IDT for Illumina-Nextera DNA Indexes (Plate C); Size Selection: size selection was performed using Ampure XP beads to select for fragments between200-800 bp; Quality and quantity was assessed using the Agilent Bioanalyzer. Aim of Sequencing: to asses chromatin accessibility; sample Type: single cell, with 50,000 cells per sample; Sequencing platform: BGI; Read Type: Paired-end; read length: 2x 100 bp; library type: ATAC-seq library; Indexing Strategy: Dual-indexing strategy using the IDT for Illumina-Nextera DNA UD Indexes (Plate C).