Oxford Nanopore whole-genome sequencing of Linum usitatissimum convar. crepitans: variety K-1531
DNA was extracted from nuclei isolated from flax leaves; DNA libraries were prepared with the SQK-LSK109 and SQK-LSK114 ONT kits and sequenced on the R9.4.1 and R10.4.1 flow-cells (400 bps); fast5 files were basecalled using Guppy 6.4.6 and two config files (dna_r9.4.1_450bps_sup.cfg, dna_r10.4.1_e8.2_400bps_sup.cfg) at a basecalling quality threshold of 8 (min_qscore = 8); fastq files from the "pass" folder were merged