GSM7720110: oe_pankla_npb_rep1; Gallus gallus; OTHER
Embryos were collected in Ringer's solution when they reached HH6 and were dissected to enrich for neural plate border cells as previously described (http://www.genome.org/cgi/doi/10.1101/gr.249680.119). CUT&RUN for PanKla (n=2 embryos/sample) were performed on control and experimental samples as described previously. DNA libraries for CUT&RUN experiments were performed using the NEBNext Ultra II DNA Library Prep Kit (NEB, E7645) following the manufacturer's protocol. Quality control of prepared libraries was performed using an Agilent 4200 Tapestation with D5000 Reagents (Agilent Part# 5067-5589) and D500 ScreenTape (Agilent Part# 5067-5588). Libraires were pooled to equimolar concentrations using the NEB Library Quantification Kit (NEB E730S) and sequenced with paired-end 37-bp reads on an Illumina NextSeq 500 instrument.