GSM7500887: PBS_RNA_rep3; Mus musculus; RNA-Seq
Cellular suspensions were loaded onto a Chromium Controller (10x Genomics) to generate nanoliter-sized gel bead-in-emulsions (GEMs) containing single cells, reagents, and a single gel bead containing barcoded oligonucleotides. Lysis and barcoded reverse transcription of polyadenylated mRNA from single cells were performed inside each GEM. Barcoded sequencing libraries were prepared using Chromium Next GEM Single Cell 3' v3.1 Dual Index (10X Genomics) according to the manufacturer's protocol.