GSM7846657: areBΔ, fructose/NH4, biol rep2; Aspergillus nidulans; RNA-Seq
Total RNAs were isolated according to (Schmitt et al., 1990) and then treated with TURBO™ DNase (Invitrogen). Ribosomal RNA was depleted with Ribominus™ Transcriptome Isolation Kit Yeast (Invitrogen). Sample quality was assessed using an Agilent 2100 Bioanalyzer and RNA 6000 Pico kit. 24 RNA-Seq libreries were constructed using rRNA-depleted RNAs and KAPA RNA HyperPrep (Kapa Biosciences) kit and Illumina TruSeqTM DNA UD Indexes (Illumina). 14 cycles of cDNA amplification were applied. The size distribution was assessed using the Agilent 2100 Bioanalyzer and High Sensitvity DNA kit (Agilent). The quantity of each library was assessed by qPCR using the Kapa Library Quantification kit (Kapa Biosciences). The libraries were sequenced on the Illumina NovaSeq 6000 instrument using NovaSeq 6000 S1 Reagent Kit (200 cycles) (Illumina) (2x100 bp paired-end).