DEAD-box ATPase Dbp2 mediates mRNA release after 3'-end formation
DEAD-box ATPase Dbp2 mediates mRNA release after 3'-end formation
Cells were grown in YES and harvested by centrifugation after crosslinking with 1% formaldehyde for 20 min at room temperature. ChIP experiments were performed as described in Wittmann et al., Nat Commun 8, 14861 (2017). HTP-tagged proteins were immunoprecipitated with IgG-coupled Dynabeads (tosylactivated M280, Thermo Scientific). Sequencing libraries were constructed with the TruSeq ChIP Library Preparation Kit (Illumina).