GSM7501617: VarsSeqDNA; Saccharomyces cerevisiae; OTHER
DNA was extracted using the YeaStar Genomic DNA Kit (Zymo Research). We carried out a 7 cycle indexing PCR to amplify target intron library regions (complete variant sequences and barcode sequences) and add i5/i7 indices for demultiplexing sub-libraries in Illumina sequencing. We then amplified our samples for 11-14 PCR cycles with P5/P7 primers to obtain sufficient material for Illumina sequencing, and we size-selected the final library using RNACleanXP beads (Beckman Coulter).