GSM7846480: sgNT_3; Homo sapiens; RNA-Seq
RNA was extracted from LNCaP/AR cells using Trizol (Ambion, 15596018). RNA-seq libraries were then prepared using the stranded Illumina TruSeq mRNA kit. The preparation began with 500 ng of total RNA and included 10 cycles of PCR amplification These barcoded RNA-seq libraries were run as paired-end, 50-nucleotide reads on an Illumina HiSeq 2500 and were selectively filtered by poly(A).