GSM7093707: Ishikawa H3K4me1 DMSO; Homo sapiens; ChIP-Seq
Cells were fixed in 1% formaldehyde for 10 minutes at room temperature and then treated with 125 mM Glycine for 5 minutes to stop crosslinking. Crosslinked cells were then washed with cold PBS and scraped to harvest. ChIP and library construction was performed as described in Reddy et al, Genome Research, 2009.