GSM7813628: Lepr-cKO 4wpc contra [KO-177Snc]; Mus musculus; RNA-Seq
Tissues were microdissected in ice-cold PBS and stored at -80°C in 1ml TRIZOL (Thermofisher). RNA was extracted using the illustra RNAspin Mini RNA isolation kit from GE Healthcare (25-0500-70). RNA-sequencing was performed  at the NGS- Integrative Genomics (NIG) Core Unit Institute of Human Genetics of University Medical Center Göttingen (UMG). RNA libraries were prepared for sequencing using standard Illumina protocols