GSM6460134: mEC-TW1-YMK5; Mus musculus; RNA-Seq
ECs from skeletal muscles (gastrocnemius, tibialis anterior, or quadriceps) from hindlimbs were isolated and stained with specific antibodies of CD31 and CD45, and  the pure muscle ECs (CD31+CD45-DAPI-) were sorted using MoFlo Astrios (Beckman Coulter) as mentioned above. The sorted ECs were used for total RNA extraction by TRIzol Reagent (Invitrogen, 15596026) and the RNA was treated with DNase and its quality was evaluated with gel QC. Bulk RNA sequencing for 4-6 samples (each sample indicates a mouse) with 4 time points was performed with oligo-dT mRNA directional using Illumina Novaseq (HiSeq SR50) for coding genes at Genomic Facility in University of Chicago.