GSM8098214: Aged,Lymphoma,ATACseq,rep2; Mus musculus; ATAC-seq
To assay accessible chromatin, nuclei were isolated from 75,000 isolated CD4+ T cells and ATAC-seq libraries were prepared using the ActiveMotif ATAC-seq Kit following the manufacturer's protocol (ActiveMotif, Carlsbad, CA). Following tagmentation and PCR amplification, the libraries were assessed for the expected size distribution on the Agilent TapeStation 4200 (Agilent Technologies) and quantified using the Kapa Library Quantification Kit (Roche). The final libraries were normalized, denatured, and sequenced on the Illumina NextSeq 2000 sequencer using a P1-100 flow cell to generate approximately 35-40M million 60-base read-pairs per sample (Illumina, Inc.).