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Microbial diversity is based on the Illumina NovaSeq sequencing platform, using Paired End sequencing to construct a small fragment library for sequencing. By concatenating, filtering, clustering, or denoising Reads, and conducting species annotation and abundance analysis, the species composition of the samples can be revealed; Further progress  Alpha Diversity analysis  Diversity analysis (Beta Diversity), significant species difference analysis, correlation analysis, functional prediction analysis, etc. can be used to explore the differences between samples.