RNA-Seq of mus musculus: adult female colon
Fresh colonic samples of Control and Infected groups were collected to analyze the whole profile of transcriptome. Sequencing and data analysis were conducted in CapitalBio Technology Co., Ltd. Total RNA extracted from each sample using Trizol reagent kit (Invitrogen, Carlsbad, CA, USA) at 4 C, according to the manufacturers instruction. Thereafter, the total mRNA was enriched by Oligo(dT) magnetic beads. The mRNA sequences were fragmented into debris by using fragmentation buffer and reverse-transcribed into first-strand cDNA, following by second-strand cDNA amplification by polymerase chain reaction (PCR). The ligation debris were size selected by agarose gel electrophoresis and amplified and sequenced by using Illumina HiSeqTM 2501 platform by Gene Denovo Biotechnology Co. (Guangzhou, China).