GSM8098156: A158_EVT; Homo sapiens; RNA-Seq
Following trypsin/collagenase digestion, cells were purified by immunomagnetic separation using anti-HLA-G (EVT) or anti-integrin ß4 (CTB, vCTB). RNA was extracted using the miRNeasy Micro kit (Qiagen) according to the manufacturer's instructions. Genomic DNA was enzymatically digested by DNase I treatment and total RNA was captured by column purification. Ribosome-depleted RNA was used to make cDNA libraries using NEBNext Ultra II RNA Library preparation kit for Illumina and NEBNext Multiplex Oligos for Illumina as per manufacturer's protocol (New England Biolabs, Ipswich, MA)