GSM8283311: Con1; Homo sapiens; OTHER
At the end of the screen, harvest genomic DNA (gDNA) from a sufficient number of cells to maintain a coverage of >500 using the Zymo Research Quick-gDNA MidiPrep according to the manufacturer's protocol. Elution was performed twice with 150 μl each for maximum recovery of gDNA. Amplify the sgRNA for NGS. Scale up the number of reaction such that all the gDNA harvested from the screen is amplified. Each 50-μl reaction can hold 1 μg of gDNA. Using a illlumina NovaSeq6000 to perform the NGS for sgRNAs. DNA libraries were prepared for sequencing using standard Illumina protocols