Illumina NovaSeq 6000 paired end sequencing; scRNAseq of freshly dissociated and cultured skin biopsies
scRNAseq of freshly dissociated and cultured skin biopsies
freshly dissociated and cultured skin samples were processed as described in [PAPER REFERENCE] scRNA-seq libraries from skin cells were prepared using the single-cell 3' v3.1 protocols and reagents (10XGenomics) according to the manufacturer's instructions. 6,000 single skin cells were targeted for the encapsulation. The quality and quantity of cDNA and scRNA-seq libraries were assessed with Agilent Bioanalyzer (Agilent Technologies)