Amplicon Sequencing of SARS-CoV-2: Wastewater
Wastewater viral particles captured using ceres microbiome A magnetic beads, extracted with RNEasy mini kit (Qiagen). Lunascript RT supermix (NEB) and PCR amplified using Q5 2x Mastermix (NEB) and ARTIC V4 primers. Library prep using Illumina DNA prep kit.