Mito DNA - Seq
Primers for the amplification of the complete mitochondrial genome sequence of Gobio huanghensis were designed by prime-blast. The total volume of PCR reaction was 50 L using a BIO-RAD PCR apparatus. PCR amplification products were detected by 1% agarose gel electrophoresis. The PCR product of the target fragment was sent to Tianyi Huiyuan Biotechnology Co., LTD. (Wuhan) for sequencing.