RNAseqofHomosapiensmelanoma sample
SampleswereFACSsortedtoobtainLin+andLin-fractions.RNAwasextractedfromsampleswithQiagen'sRNAeasyPluskit,ribosomallydepletedusingTakara'sRibogoneMammaliankit.cDNAwasmadeusingClontech'sSMARTerUniversalLowInputRNAKitforSequencing,andbarcodeswereaddedusingtheIonPlusFragmentLibraryKit.TwoindividuallybarcodedsamplesweresequencedtogetheronanIonTorrentS5XLbytheAnalyticalandTranslationalGenomicsSharedResourceatUNMCCC.