GSM7498255: WT Striatum Female rep3 [Msh3]; Mus musculus; ATAC-seq
Brain tissues were dissected from mice at 12 months of age. Nuclei were dissociated from frozen striatal tissues and labeled with Alexa 488-conjugated anti-NeuN antibody. Neuronal nuclei were then harvested by flow cytometric sorting. 50,000 nuclei for each sample were used for transposition reactions and library prep. The transposition reactions and ATAC-seq library preparation generally followed the steps set out in the original ATAC-seq paper using Nextera DNA Library Preparation Kit.