Whole Genome Shotgun Sequencing of Aspergillus parasiticus
The genomic and mitochondrial DNA of Aspergillus parasiticus grown on malt extract agar was extracted using the NucleoSpin plant II kit (Macherey-Nagel). The quality and quantity of the DNA was checked using a NanoDrop 1000 (Thermo Fisher Scientific) and a fluorometer (Qubit 3.0, Thermo Fisher Scientific). The library was prepared using the Illumina DNA Prep kit and the Illumina Nextera DNA CD Indexes. The quality of the library was checked using an experion automated electrophoresis system (Bio-Rad). Then, the library was paired-end sequenced using an Illumina MiSeq instrument.