DNA sequence of mustard greens planted soil from each individual plot C1
The V4 variable region of the 16S rRNA gene was amplified by using the 515F and 806R specific primer. PCR reactions were carried out with Phusion High-Fidelity PCR Master Mix. The PCR products were purified using Qiagen gel extraction kit. The libraries were generated with TruSeq DNA PCR-Free sample prepara-tion kit and analyzed by HiSeq2500 according to the manufacturers instructions.