GSM6598757: LCS8269_DR_WT01; Mus musculus; RNA-Seq
QIAGEN RNEasy kit RNA integrity was checked with Agilent Technologies 2100 Bioanalyzer. Poly(A) tail-containing mRNAs were purified using oligo-(dT) magnetic beads with two rounds of purification After purification, poly(A) RNA was fragmented using divalent cation buffer in elevated temperature. The DNA library construction is shown in the following workflow. Quality control analysis and quantification of the sequencing library were performed using Agilent Technologies 2100 Bioanalyzer High Sensitivity DNA Chip a Poly(A) RNA sequencing library was prepared following Illumina's TruSeq-stranded-mRNA sample preparation proto