Illumina paired end sequencing
Leiomyosarcoma
Ribosomal RNA depletion was performed using QIAseq FastSelect RNA Removal Kit. cDNA libraries were prepared by NEBNext Ultra II Directional RNA Library Prep Kit (Cat# 7760S) using 500 ng RNA, according to the manufacturer's protocol. Libraries were sequenced using Illumina NovaSeq6000 (2 x 151 bp). Fusion events were identified using STAR-Fusion (version 1.1.0) with the following default parameters: --FusionInspector inspect --annotate --examine_coding_effect --extract_fusion_reads. Key genes involved in DNA repair and PLK4 analysed in this study was extracted in the final BAM files.