GSM7720753: CRISPRi human, cyno (GEX); Macaca fascicularis; Homo sapiens; RNA-Seq
Generate single cell suspension with Accumax, wash cells with PBS+0.04 % Bsa and also store in that buffer until use According to the manufaterer's instructions (Chromium Next GEM Single Cell 5' Reagent Kits v2 (Dual Index); CG000510 Rev B) 5' scRNA-seq based on polyA priming and CRISPR libraries from the gRNA based on capture of constant gRNA region