GSM6914704: KO1_IP; Mus musculus; ChIP-Seq
cell was crosslinked in 1% formaldehyde for 10 min at room temperature. The crosslinking reaction was terminated by 0.125 M glycine for 5 min at room temperature. Then the nucleus was extracted form lysates by centrifuging at 2000g for 5min and lysed and sonicated to fragment chromatin DNA. The sonicated chromatin was incubated with anti-ubH2B antibody. The DNA fragment was extracted by phenol-chloroform method. The sequencing libraries were prepared using VAHTS Universal DNA Library Prep Kit for Illumina V3 (Vazyme, ND607).