16S rRNA transcript amplicon, sediment : 32 cm depth
RNA was extracted from 1 g of wet sediment using theRNeasy PowerSoil Total RNA kits (Qiagen), treated with DNase 1 prior to the  retro-transcription using the PrimeScript RT Reagent Kit (Takara) and PCR amplification were done using the universal primer pair 515F / 806R