GSM6460032: Mouse CRC tissue was obtained from wild-type C57 mice with AOM/DSS model, PBS6; Mus musculus; RNA-Seq
Chromium™ Single Cell 3'Solution is a microfluidics platform built on GemCode technology that combines gel beads with barcodes and primers. Individual cells are encapsulated in oil droplets to form GEM(Gel bead in emulsion).The gel beads in the GEM dissolve and the cells cleave to release mRNA, which is then reverse transcribed to produce cDNA with barcodes for sequencing. After the liquid oil layer is destroyed, cDNA amplification reaction is carried out. After purification, quality inspection is carried out. Enzymatic digestion interrupted the fragments, end patching was carried out, and base A was added to connect the joints, and the target size fragments were recovered for library quality inspection and quantification.