DNA-Seq of Mesocricetus auratus: adult male feces
The quality and quantity of the extracted DNA were examined using Qubit dsDNA HS Assay Kit on a Qubit 3.0 Fluorometer (Life Technologies, Carlsbad, CA, USA) and electrophoresis on a 1% agarose gel, respectively. Paired-end libraries (insert size, ~350 bp) were prepared using a VAHTS Universal Plus DNA Library Prep Kit for Illumina (Vazyme Biotech). The library was sequenced on an Illumina NovaSeq 6000 platform (Biomarker Technologies Co., Ltd., Beijing, China) using the 150-bp paired-end sequencing mode. The two paired FASTQ files were base called from the Illumina raw sequence read data. The quality of the raw sequence reads was assessed using Trimmomatic v0.33 was used to trim sequencing adapters, reads with a quality score <20 over a sliding window size of 50 bp, and reads with a sequence length <100 bp. After trimming the adaptors and filtering low-quality reads, the clean sequence data were used for further bioinformatics analyses.