RNA-Seq of Rattus norvegicus: trigeminal neuralgia
RNA preparation, library construction, and sequencing were performed by BGI GENE using high throughput genome sequencing platform BGISEQ-500. In brief, the trigeminal nerve root tissue was harvested immediately after the rats were euthenized under excessive anesthesia. The samples were homog enized in Trizol (Invitrogen, California, USA). Total RNA was generated according to the protocol. Quantitative and qualitative analysis of RNA was performed by a Qubit Fluorometer (Agilent Technologies, California, USA). In order to determine hether the sequencing data were suitable for subsequent analysis, raw RNA  sequencing data required quality control (QC) on the raw reads. The resulting clean data were aligned to the reference sequence and low-quality reads were discarded after QC.