GSM8186611: MAC cells, NT MAG Exomyo, 3 injections, biol rep 2; Mus musculus; RNA-Seq
RNeasy Micro kit (QIAGEN). RNAs were qualified and quantified with AGILENT Tapestation 2200 on High Sensitivity RNA chip. To prepare the library and sequence the DNA, 150 to 300 ng of total RNA from samples was determined by InvitrogenTM QubitTM; high-sensitivity spectrofluorometric measurement was poly-A selected and reverse transcribed using Illumina's TruSeq stranded mRNA library preparation kit. Each sample was fitted with one of 96 adapters containing a different 8 base molecular barcode for high level multiplexing and libraries were sequenced on an Illumina NovaSeqTM 6000.