RNA-Seq of Danio rerio 7dpf larvae
total RNA was extracted from whole larvae using a the TRIzol Plus RNA Purification Kit with Phasemaker tubes (Thermo Fisher) due to the small size of the larvae. RNA concentration was measured using Qubit RNA HS Assay Kit (Thermo Fisher Scientific) and quality assessed using TapeStation (Agilent). RNA was sequenced at 150bp paired end on an Illumina NovaSeq at the Centre for PanorOmic Sciences (CPOS) of the University of Hong Kong