GSM6636227: E26_tumour_CL_RNAseq; Homo sapiens; RNA-Seq
RNA extracted from cells using the QIAGEN RNeasy kit. Samples prepared with KAPA mRNA Hyper prep kit with KAPA SeqCap Adapters. Fragmentation 94 deg C for 6 mins, library amplification of 12 cycles. Each library quantified with Qubit dsDNA HS assay, average fragment size calculated with Bioanalyzer DNA 1000 or DNA HS. Molarity of each library was calculated, normalized to ∼10 nM and pooled, 24 libraries per pool. Each pool quantified using Qubit dsDNA HS assay, average fragment size determined using Bioanalyzer DNA HS. Further detail at Gangoso et al, Cell, 2021.