GSM6912641: normal children, 2-4 years old [Sample C1]; Homo sapiens; miRNA-Seq
TRIzol LS Reagent (Invitrogen life technologies: 10296028) was used for isolation of RNA from exosomes. The integrity of RNA was confirmed via a 2% agarose gel, and the concentration (OD 260) and protein contamination (Ratio OD260/OD280) of the total RNA samples were determined with NanoDrop ND-1000. The quality and concentration of the sequencing library were assessed with Agilent 2100 bioanalyzer. According the manufacturer's protocol, sequencing was performed based on Illumina NextSeq 500.