Ion Torrent Proton sequencing; RNA-Seq of human glioblastoma cells with knockdown of FGFR1 or FGFR2
RNA-Seq of human glioblastoma cells with knockdown of FGFR1 or FGFR2
Cultured human glioblastoma cell pellets were snap frozen until RNA extraction. Human glioblastoma cells were grown in N2 medium containing 20 ng/ml recombinant human EGF and 2% bovine serum albumin. Prior to RNA extraction, human glioblastoma cells were treated with 20 ng/ml recombinant human FGF2 for 48 hours. Total RNA was extracted using Qiagen RNeasy kit according to manufacturers instructions. Library preparation for RNA sequencing was performed using the Ion Total RNA-Seq V2 kit and Ion Xpress RNA-Seq Barcode kit (Thermo Fisher).