Neuron specific RNA-seq of C. elegans Day 8 him-8;daf-22 male neurons: replicate 2
RNA-seq was performed on him-8;daf-22 neurons isolated from Day 2 male C. elegans. Male population was enriched through filtering. Samples with a male population > 95% are kept. Total RNA from FACS-isolated,Punc-119::gfp labeled neuron samples was isolated and extracted and library was generated with ribosomal RNA depletion using Ovation SoLo RNA-Seq library preparation kit from Tecan Genomics. Sequencing was performed on Illumina Novaseq 6000 S1 100nt platform.