WASP-KO H3K27ac
Three million WASP-KO macrophage cells were cross-linked using freshly prepared PFA, utilizing the EZ-Magna ChIP HiSens Chromatin Immunoprecipitation Kit (EMD Millipore, 17-10461). Sonication was performed using a Bioruptor Pico sonication device with 30 seconds on and 30 seconds off cycles for 50 cycles. 5 g of H3K27ac antibody (Active motif, 39113) was utilized. DNA samples were purified using the ChIP DNA Clean and Concentrator Kit (D5205, Zymo Research) and eluted in 15l of Elution buffer. Library preparation was carried out using the MicroPlex Library Preparation Kit v2 (12 indexes) (Fisher Scientific, C05010012). The library samples were sequenced at paired-end 150bp on an Illumina HiSeq 4000 sequencer. WASP-KO H3K27ac ChIP-Seq reads were mapped to the hg38 genome using DNASTAR SeqMan NGen v2.5.1 with default settings.