GSM8395989: SUM159_pLVX_FLAG_POGK_repl1, ChIP; Homo sapiens; ChIP-Seq
Each reaction includes 5X106 cells, initially crosslinked with 1% formaldehyde and subsequently quenched using glycine. The crosslinked cells underwent sonication to achieve DNA fragmentation within the range of 200 to 350 base pairs. Immunoprecipitation of DNA was then conducted utilizing magnetic antibody beads. Following reverse crosslinking, DNA purification was carried out using the Qiagen PCR purification kit. The DNA quantity was validated using the 2100 Bioanalyzer expert High Sensitivity DNA Assay. Each reaction includes ~50 ng of DNA and undergoes library preparation and sequencing conducted by Azenta NGS laboratory (Waltham, MA) utilizing the Illumina HiSeq platform configured with 2x150 base pairs. The sequencing depth achieves over 20 million reads per sample. The sequencing data are aligned to the human genome hg38 for analysis.