GSM6398523: WT3; Mus musculus; RNA-Seq
Cells were lyzed and mRNA extracted using Qiagen RNEasy Mini kit including on-column DNAse digestion. Libraries were prepared according to Illumina's instructions accompanying the TruSeq Stranded mRNA Library Prep Kit (Illumina). 500 ng of RNA was used for each sample. Sequencing was performed in 1 sequencing unit of NovaSeq 6000 (Illumina) (100-nt-length reads, paired end).