GSM7093693: IR; Mus musculus; RNA-Seq
Heart tissues of C57BL/6 mice was gently washed with cold PBS and cut into 4-6 mm3 pieces,tissue placed cut side into a plastic mold.  The OCT-filled mold was then snap frozen in chilled isopentane.Cryosections were cut at 10-µm thickness (Leica,cm1950).Brightfield histology images were taken using a 20x objective on Pannoramic MIDI scanner (3D HISTECH). Raw images were stitched together using pannoramic MIDI and  exported as .tiff files with low- and high-resolution settings. For tissue optimization experiments, fluorescent  images were taken with a TRITC filter using a 20x objective. Libraries were prepared according to the Visium Spatial Gene Expression User Guide (CG000239:https://assets.ctfassets.net/an68im79xiti/3pyXucRaiKWcscXy3cmRHL/a1ba41c77cbf60366202805ead8f64d7/CG0  00239_VisiumSpatialGeneExpression_UserGuide_Rev_A.pdf). They were loaded and sequenced on a  NovaSeq 6000 System (Illumina).The sequencing and bioinformatics analysis were performed by Majorbio Co., Ltd (Shanghai, China). scSpatial-seq