GSM7502469: Carotid plaque donor 2 not_infected [2-Donor-2-not-infected_S2]; Homo sapiens; RNA-Seq
Total RNA from human atherosclerotic tissue was isolated using QIAzol Lysis Reagent and the gentleMACSTM Octo Dissociator (Miltenyi Biotec) homogenizer, combined with RNA clean-up using the RNAeasy Mini kit. For human atherosclerotic plaqueex-vivoexperiments, libraries were prepared following the Revelo RNA-Seq High Sensitivity library preparation kit according to manufacturer's instructions. Libraries were diluted and quantification was performed using KAPA library Quantification kit. Prepared libraries were pooled at2nMequimolar concentration and sequenced using the Illumina NovaSeq 6000 sequencer(100 cycles with S1 workflow).