Metagenomic sequencing from 360mL of Trout Bog Lake water at a depth of 5m
Illumina TruSeq Nano DNA Library Preparation Kit, with IDT for Illumina TruSeq Unique Dual Index Adapters following manufacturers recommendations. Completed libraries was QCd and quantified using a combination of Qubit dsDNA HS, and Agilent 4200 TapeStation HS DNA1000. This pool was combined with other libraries prepared within the Genomics Core to share sequencing on a NovaSeq S4 flow lane using 2/10th of a lane (400M read pairs). The mixed pool was loaded onto one lane of an S4 flow cell and sequencing was performed in a 2x150bp paired end format using a NovaSeq v1.5 300 cycle reagent cartridge.