Neuron specific RNA-seq of C. elegans wild-type N2 neurons: replicate 5
RNA-seq was performed on N2 neurons isolated from Day 8 adult C. elegans. Total RNA from FACS-isolated,Punc-119::gfp labeled neuron samples was isolated and extracted and library was generated with ribosomal RNA depletion using Ovation SoLo RNA-Seq library preparation kit from Tecan Genomics. Sequencing was performed on Illumina Novaseq 6000 S1 100nt platform.