GSM6697893: 85_YFP_P; Mus musculus; RNA-Seq
Tregs were isolated from the spleens of SRC-3KO and WT animals and sorted for YFP. Total RNA was isolated from YFP+ AND YFP- Tregs using Qiagen micro RNAesy plus kit. Quality control of isolated RNA samples was checked using Agilent Bioanalyzer. Takera SMART-Seq v4 kit was used to generate the low throughput sequencing libraries. RNA libraries were prepared using Takara Bio SMART-seq v4 plus by manufacturer recommended protocol.