GSM8143792: A375 WT AGO1-4 IP rep 2; Homo sapiens; RNA-Seq
Cells were fractinated into cytoplasmic and nuclear lysates and clarified by centrifugation. First AGO proteins were enriched by immunoprecipitation with T6B peptide. After washing the beads they were treated with Trizol and the RNA extracted. 3'adapter and 5'adapter were added generating small RNA cDNA library preparation Ligation products were reverse transcribed and amplified by PCR.