GSM7847075: H520_T5_BaP; Homo sapiens; RNA-Seq
Total RNA was isolated using Qiagen RNeasy kit, and mRNA was subsequently isolated using PolyA purist MAG kit. cDNA was synthesized from mRNA using Superscript III reverse transcriptase, from which short sequences encompassing 12 bp unique tags were amplified using Q5 high-fidelity polymerase and primers introducing Illumina TruSeq adapter sequences.