16SrRNA of human feces : in chronic kidney diseases
A 16S rRNA gene fragment comprising V3 and V4 hypervariable regions. ( f- CCTACGGGNGGCWGCAG r- GACTACHVGGGTATCTAATCC) was amplified using an optimized and standardized 16S-amplicon-library preparation protocol. Sequencing was then performed using a 250-bp paired-end sequencing protocol on an Illumina MiSeq platform