GSM6914486: scRNA_TxnipWT_effector_Th2_rep2; Mus musculus; RNA-Seq
The scRNA-seq samples of Th2 at day7 and day11 in vivo that were freshly prepared from KJ1+CD4+ T cells sorted using a BD FACS Aria III (10,000 cells each, cell viability >98%). Libraries were prepared using Chromium Single Cell 3' Reagent Kits v3.1 according to manufacturer's protocol (10X Genomics). The generated scRNA-seq libraries were sequenced using a 128 cycle (paired-end reads) with a NovaSeq 6000 (Illumina).