GSM8008700: WGBS_DNMT3A+DNMT3L-ADD_Sperm_Rep2; Mus musculus; Bisulfite-Seq
FGOs were harvested from adult ovaries (10 to 12 weeks old) by needle puncture and cumulus cells were removed by capillary washing. Sperms were released from the cauda epididymides of adult male mice and collected by swim-up method. For the 3a/3L DM sperm, viable sperms were selected by mouth pipetting Sample with unmethylated lambda phage DNA was subjected to bisulfite treatment and library construction by the post-bisulfite adaptor tagging (PBAT) method (Miura et al., 2012). Bisulfite-seq (PBAT method)