RNA-Seq of region NIVO22-RA
Libraries for whole transcriptome RNA-seq were generated with Illumina TruSeq Stranded Total RNA. 100 ng RNA was used as input for Ribo-Zero rRNA Removal Kit, with Illumina TruSeq RNA UD Indexes (96 indexes) for sample indexing. Qubit dsDNA High Sensitivity assay (Thermo Fisher Scientific) was used for library quantification. Sequencing was done on Illumina NovaSeq™ 6000 S2 (36-plex) or S4 (72-plex) flow cell with 76 bp paired-end sequencing to produce ~200 million paired reads per library.