GSM7720369: Adipose tissue,replicate2, scRNA_seq; Mus musculus; RNA-Seq
The single-nucleus suspension was adjusted to a concentration of 3-4 × 105 nuclei/mL in PBS. This suspension was loaded onto a microfluidic chip, utilizing the GEXSCOPE® Single NucleusRNA-seq Kit (Singleron Biotechnologies). Following the manufacturer's guidelines (Singleron Biotechnologies), we proceeded to construct the snRNA-seq libraries. These libraries were sequenced on an Illumina HiSeq X10 platform, using 150 bp paired-end reads.