GSM6734647: A_HCC_Input; Homo sapiens; ChIP-Seq
Genomic DNA degradation and contamination were monitored on 1% agarose gels. DNA purity was checked using the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA). The DNA concentration was measured using a Qubit® DNA Assay Kit in a Qubit® 2.0 Fluorometer (Life Technologies, CA, USA). A total amount of 50 ng DNA per sample was used as input material for the ChIP sample preparations. The Chip-Seq library construction mainly includes the steps of cell cross-linking, cell lysis, protein co-immunoprecipitation, extraction of co-precipitated complex DNA, and construction of DNA next-generation sequencing library. Sequencing libraries were generated using NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample.