SHAPE-MaP of Ty3 in vitro 5' end transcript: dimer NAI rep1
RNAwastranscribedinvitro,refoldedandmodifiedbySHAPE in specified conditions.ThenRNAwasreversetranscribedwithspecificprimerinMn2+condtions,anddsDNAwasmadebyampliconPCR.Ampliconlibrarywasfurtherperformed.