GSM8008154: HEK293T_ChIP_H3K9me3_NC_ASO_rep3; Homo sapiens; ChIP-Seq
1% Formaldehyde (Sigma, F8775) was added into each well to crosslink the cells for 10 min with a slight shaking at room temperature.chromtin was sonicated using Covaris M220 sonicator. DNA was isolated by performing a phenol/chloroform/isoamyl alcohol extraction to prepare the ChIP-seq library using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB, E7645).