GSM7497999: CSF cell specimen; Homo sapiens; RNA-Seq
All sorted samples were pre-gated on CD3+, CD4+ or CD8+, live, single-cells. 5,000 sorted cells from each CD4+ and CD8+ population were pooled and used for scRNA-Seq analysis as below Cells FACS sorted into DMEM with 40% FBS were used for scRNA-Seq sample preparation. After sorting, cell number was confirmed with the Luna II (Logos Biosystems). The appropriate number of cells necessary to achieve a target cell input of 5,000 cells was resuspended and used to generate GEMs (Gel Bead-In Emulsion). The Chromium Next GEM Single-cell 5' Gel beads v2 kit (10X Genomics) was used and manufacturer instructions were followed for GEM generation through cDNA synthesis. A Chromium Controller was used for GEM generation and barcoding then a thermocycler was used for cDNA synthesis. cDNA was submitted to the University of Minnesota Genomics Center for sequencing on an Illumina Novaseq. Tetramer-enriched scRNA-seq data pooled from three biological replicates.