GSM8395524: Control-3; Mus musculus; RNA-Seq
BMDMs were washed with PBS, lysed in Trizol (9108, Takara, Japan), and sent for next-generation sequencing. RNA was quality-assessed with Agilent 2100 (Agilent, USA) using RNA 6000 Nano kit (5067-1511, Agilent, USA), with RNA integrity number above 9 for library construction. The total RNA samples from three independent experiments were used for library construction according to the protocol of Illumina NovaSeq 6000 platform and then sequenced on a Thermal Cycler (Eastwin, Suzhou, China) using HighSensivity DNA assay Kit (5067-4626, Agilent, USA). The FASTq sequencing files were aligned to the mouse reference genome (GRCm38, mm10) using STAR aligner for further analysis.