GSM6568632: U251 cells, ST101, biological replicate 1; Homo sapiens; RNA-Seq
Cell pellets were resuspended in RNAlater (Thermo Fisher Scientific, USA) and total RNA was extracted using Qiagen RNeasy Protect Mini Kit (Qiagen Inc. USA) according to manufacturer's  instructions and treated with Qiagen RNase-free DNase to remove genomic DNA. RNA libraries for RNA-seq were prepared using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® following manufacturer's protocols