ddRAD sequencing using restriction enzymes MseI+EcoRI of Lepidochelys olivacea: female adult tissue
1 g of genomic DNA (20-100 ng/l) was digested with EcoRI and MseI at 37 C for at least two hours. Ligated samples were cleaned with 1.8X CleanPCR magnetic beads from CleanNA. A ten-cycle indexing PCR was performed independently for each individual using one of the 50 indexes described by Meyer and Kircher (2010) at the P7 adapter. The size selection step was performed using the BluePippin with a 1.5% agarose cassette (250-1,500 bp) and R2 marker (Sage Science) and a size range of 450-650bp was selected. The libraries were run on the Illumina MiSeq using the 600-cycle v3 kit