GSM7417951: C9-4_LPS_matched_vehicle, iPSC-MG, C9orf72, day 28-30/40-42; Homo sapiens; RNA-Seq
RNA was isolated with RNeasy Mini Kit (QIAGEN-74104) following the manufacturer's instructions. RNA samples were measured for quantity with Quant-iT Ribogreen RNA Assay (Thermo Fisher, Cat. No. R11490) and quality with Agilent High Sensitivity RNA ScreenTape and buffer (Agilent, Cat. No. 5067-5579 & 5067-5580). For each RNA sample, an indexed, Illumina-compatible, double-stranded cDNA whole transcriptome library was synthesized from 1 μg of total whole transcriptome library was synthesized from 1 μg of total Prep Kit – HI Mammalian (Takara Bio, Cat. No. 634876) and SMARTer RNA Unique Dual Index Kit (Takara Bio, Cat. No. 634418). Library preparation included ribosomal RNA depletion, RNA fragmentation (94◦ C for 3 min), cDNA synthesis, and a 12-cycle unique dual indexing enrichment PCR.