female SPRAGUE-DAWLEY rat, age 2 year LIVER
All samples were prepared with the Kapa Hyper Library Preparation kit (Roche, catalog no: 07962371001) following the manufacturers instructions in batches of 95 plus a no template control. All samples underwent amplification, with cycle number dependent on the concentration of DNA, normalized to 2.5 nM and sequenced in pools of 14 samples on the NovaSeq 6000.