Bulk RNA-seq of 6 week old IRF5 WT mice in basal mammary epithelial cells replicate 2
Luminal epithelial and myoepithelial cells were sorted from three mice per age and genotype. RNA extraction was performed with the RNAeasy mini kit (Qiagen). Double stranded cDNA synthesis and Illumina libraries were prepared utilizing the Ovation RNA-seq system (V2) (Nugen Technologies, #7102-32). RNA-seq libraries were prepared utilizing the Ovation ultralow DR multiplex system (Nugen Technologies, #0331-32). Each library was pooled and barcoded with Illumina True-seq adaptors to allow sample multiplexing, followed by sequencing (n=4) on an Illumina NextSeq500, high output, 76bp single-end run.