C9-06
Leaf samples were collected in RNAlater solution (Thermo Fisher Scientific, USA) at room temperature and subsequently stored at -80C until RNA extraction. Each sample was georeferenced and one voucher was deposited in the HSTM herbarium (M.A.Buitrago 472). Total RNA was extracted from fresh leaves using a QIAGEN RNeasy Plant Mini Kit (Qiagen, Finland). We used agarose gel 1% stained with gel-red (Biotium Inc., USA) to check the quality and integrity of extracted RNA, and further quantified the samples using Qubit4 fluorometer Invitrogen (Thermo Fisher Scientific, USA). Samples were sent to Macrogen Inc. (South Korea) to perform the total RNA library construction using TruSeq stranded mRNA library kit. Paired-end sequencing was performed on the Illumina NovaSeq 6000 platform