GSM8240563: NSCs, Ythdf2-WT, 4 hour post Actinomydin D, RNA-seq, rep3; Mus musculus; RNA-Seq
For RNA-seq, RNA was extracted using TRIZOL and the kit of Zymo RNA Clean & Concentrator™-5. 300ng of total RNA was used for the construction of sequencing libraries. For m6A-IP-seq, RNA from WT NSCs was pulled down with m6A antibody, and the pulldown RNA was purified with Zymo RNA Clean & Concentrator™-5 and used for library preparation. For YTHDF2-RIP-seq, cell lysates  from WT NSCs were pulled down with YTHDF2 antibody, and the pulldown RNA was purified with TRIZOL and Zymo RNA Clean & Concentrator™-5 and used for library preparation RNA libraries for RNA-seq were prepared based on SMART-seq2 method. RNA libraries for m6A-IP-seq and  YTHDF2-RIP-seq were prepared based on modified SMART-seq3 method.