RNA-seq of Breast Cancer Cell Line: SCP2
The total RNA was extracted from SCP2 cells using TRIzol reagent (Takara, Dalian, China) and further purified by rRNA depletion, followed by cDNA synthesis and RNA amplification, according to the manufacturers instructions. The RNA-seq libraries were constructed and sequenced utilizing the Illumina HiSeq2500 platform (Illumina, San Diego, USA).