Dovetail OmniC sequencing data from Masticophis lateralis: liver sample from adult female (HBS 135920)
The Omni-C library was prepared using the DovetailTM Omni-CTM Kit (Dovetail Genomics, CA) according to the manufacturers protocol with slight modifications. First, specimen tissue (liver, ID: HBS 135920) was thoroughly ground with a mortar and pestle while cooled with liquid nitrogen. Subsequently, chromatin was fixed in place in the nucleus. The suspended chromatin solution was then passed through 100 m and 40 m cell strainers to remove large debris. Fixed chromatin was digested under various conditions of DNase I until a suitable fragment length distribution of DNA molecules was obtained. Chromatin ends were repaired and ligated to a biotinylated bridge adapter followed by proximity ligation of adapter containing ends. After proximity ligation, crosslinks were reversed and the DNA purified from proteins. Purified DNA was treated to remove biotin that was not internal to ligated fragments. An NGS library was generated using an NEB Ultra II DNA Library Prep kit (NEB, Ipswich, MA) with an Illumina compatible y-adaptor. Biotin-containing fragments were then captured using streptavidin beads. The post capture product was split into two replicates prior to PCR enrichment to preserve library complexity with each replicate  receiving unique dual indices. The library was sequenced at Vincent J. Coates Genomics Sequencing Lab (Berkeley, CA) on an Illumina NovaSeq platform (Illumina, CA) to generate approximately 100 million 2 x 150 bp read pairs per GB genome size.