16S rRNA v4 region gene amplicon sequencing data from Sciurus carolinensis feces
We extracted DNA from 0.2 g of feces using QIAamp DNA Stool Mini Kits (Qiagen, Hilden Germany). The v4 region of the 16S rRNA gene was amplified in triplicate (primers, 515F: 5-GTGYCAGCMGCCGCGGTAA-3; 806R: 5-GGACTACNVGGGTWTCTAAT-3) at MetaGenomBio Inc. (Waterloo, Canada). Triplicate PCR products were pooled and sequenced to a depth of 30,000 reads/sample on an Illumina MiSeq platform (v2 chemistry).