Metagenomic sequencing from 250mL of Trout Bog Lake integrated evenly between depths of 2m to 7m
Illumina TruSeq Nano DNA Library Preparation Kit, with IDT for Illumina TruSeq Unique Dual Index Adapters following manufacturers recommendations. Completed libraries was QCd and quantified using a combination of Qubit dsDNA HS, and Agilent 4200 TapeStation HS DNA1000. This pool was combined with other libraries prepared within the Genomics Core to share sequencing on a NovaSeq S4 flow lane using 2/10th of a lane (400M read pairs). The mixed pool was loaded onto one lane of an S4 flow cell and sequencing was performed in a 2x150bp paired end format using a NovaSeq v1.5 300 cycle reagent cartridge.