GSM6637542: PCM086; Homo sapiens; RNA-Seq
RNA was extracted from thawed Tempus tubes using Norgen Preserved Blood RNA kits according to manufacturer's instructions, including DNase treatment. RNA integrity was assessed using the Bioanalyser Nano Assay with all samples having RNA integrity number (RIN) 7-10. 1000 nanograms of the isolated total RNA was globin reduced from 29 of the 58 samples using the GLOBINclear™ - Human Kit. 200ng of RNA was converted to double-stranded cDNA (ds-cDNA). The ds-cDNA were subjected to heat denatured and circularised by the splint oligo sequence to generate the single stranded circle DNA followed by rolling circle replication to create DNA nanoball (DNB) using the MGIeasy RNA Library Prep. Samples were sequenced using the MGI DNBSEQ-G400RS, at two 100-base paired-end reads.