snRNA-seq of wheat seedling leaf
Libraries were constructed using the Chromium Next GEM Single Cell 3' kit v3.1 (10x Genomics), in which 25000 nuclei from 7-day-old wheat seedling leaves were used to complete GEM barcoding following the protocol recommendation. The standard protocol of Chromium Single Cell 3' Reagent Kits User Guide (Document Number CG000315 Rev E) was adopted to complete reverse transcription, pooled cDNA amplification, enzymatic fragmentation, size selection and sample index PCR. After library quality checking  by Agilent 2100 Bioanalyzer, the library was sequenced on Illumina NovaSeq 6000 platform with paired-end mode.