untrimmed paired illumina sequences of HVKP1
Library prep was carried out using the NEBNext Ultra II FS DNA library prep with sample purification beads (New England Biolabs, WI, USA) according to the manufacturers protocol for the FS DNA library prep kit (E6177) with inputs of >100 ng. The PCR enrichment step used the NEBNext multiplex oligonucleotides for Illumina (96 unique dual-index primer pairs) (New England Biolabs, WI, USA). The library prep samples were diluted to a final concentration of 1 nM with nuclease-free H2O and pooled. For loading onto the iSeq 100, the library was further diluted to 50 pM with nuclease-free H2O. Raw data was converted from BCL to fastq with BCL-Convert.