GSM6596648: ZnT3 conditional knockout in amacrine cells, primary retinal ganglion cells, optic nerve crush, day 3 [CKO1]; Mus musculus; RNA-Seq
RNA from FACS-sorted RGCs were isolated, and cDNA amplification product were prepared with Discover-sc WTA Kit V2 (Vazyme #N711), following manufacturers' protocols. RNA quality was determined by examining A260/A280 with Agilent 2100 Bioanalyzer. RNA Integrity was confirmed by 1.5% agarose gel electrophoresis. Qualified RNAs were finally quantified by Qubit3.0 with QubitTM RNA Broad Range Assay kit (Life Technologies). 2 μg total RNAs were used for stranded RNA sequencing library preparation using  the TruePrep DNA Library Prep Kit V2 (Vazyme #TD502) and TruePrep Index Kit V2 for Illumina (Vazyme #TD202), and the manufacturer's instructions.