RNA-Seq of mus musculus: KPLS neurons from E2-treated adult female OVX mice
On post-ovariectomy day 9, the mice were implanted with a single silastic capsule containing either E2 (OVX+E2 group; N=12) or a sunflower oil vehicle (OVX+Veh group; N=9) for a four-day treatment. The brains were fixed using transcardiac perfusion with 0.5% formaldehyde followed by 20% sucrose. The LS was cut into sections using a cryostat, and Laser Capture Microdissection isolated ZsGreen-tagged KP neurons. The OVX+Veh (N=3) and OVX+E2 (N=4) libraries were subjected to single-end sequencing using the Illumina NextSeq500/550 High Output Kit (v2.5; 75 cycles) and an Illumina NextSeq500 instrument.