CtIP-ODIP-Seq_Rep2
A total of 112,764 24nt ODNs, derived from ClinVar missense single nucleotide polymorphisms (SNPs), were screened. A pool of 200 ODNs was selected for ODIP-Seq. Following ODIP assay, single-stranded DNA was converted into a sequencing-compatible library. Adapter ligation, strand synthesis, and library amplification were performed using CircLigase II, Bst polymerase 2.0, T4 DNA polymerase, and T4 DNA ligase. The resulting library was then heat-denatured, releasing library molecules for next-generation sequencing.