GSM8009759: Active-Ribo-seq siPYGO2-3; Homo sapiens; OTHER
Ribosome protected fragments (RPF) was acquired using RiboLace kit (Immagina) following manufacturer's protocols. 20 ng of RPF was used for the construction of sequencing libraries. RNA libraries for Active-Ribo-seq were prepared using LACE-seq kit following manufacturer's protocols. Active-Ribo-seq