GSM6697871: Kaede-green and Kaede-red (from colon) CD45+ splenocytes, 7-wk-old Kaede.B6 mice (12 samples); Mus musculus; RNA-Seq
Immunocytes were released by mechanical disruption followed by filtering and washed in RPMI containing 5% fetal calf serum (FCS). Red blood cells were lysed prior to filtering using ACK lysing buffer Cells were then purified by fluorescence-activated cell sorting. Single cells were then sent to the Broad Institute Genomics Platform, which performed encapsulation following 10x Genomics protocols. 10x Genomics library prep, 3' v3 chemistry