GSM6735410: CD8+ T cell, Spleen, Low, Day 17, replicate 3; Mus musculus; RNA-Seq
RNA in RLT lysis buffer supplemented with β-mercaptoethanol was thawed from -80℃ and subsequently isolated using RNeasy Mini Kits (Qiagen) with on-column DNA digestion as per the manufacturer's protocols. PolyA+ Selection, Illumina TruSeq Stranded Total RNA Sample Preparation as per the manufacturer's protocols.