GSM7498792: NPA, H3K36me3, ChIP; Mus musculus; ChIP-Seq
Cells were crosslinked with 1% paraformaldehyde for 10 min at room temperature and quenched with 0.125 M glycine for 5 min. Quenched cells were washed twice with ice-cold PBS supplemented with protease inhibitors (Cell Signaling Technology). Cell pellets were mixed with provided buffers to isolate the nuclei which were then treated with 1-2 μl of micrococcal nuclease for 30 min at 37°C to digest DNA to the length of approximately 150-1000 bps followed by sonication with a focused ultrasonicator (Covaris). Isolated ChIP DNA was library prepped using the NEBNext Ultra II DNA Library Prep Kit for Illumina in accordance with the manufacturer's protocol