GSM7093611: 19_No_Tg_4; Mus musculus; RNA-Seq
Isolated glomerular tissues from 8 line (WT, G0, G1, G2, Tg26, G0xTg26, G1xTg26, G2xTg26; n=5, 3, 4, 3, 5, 5, 5, 4, respectively) were homogenized in QIAzol. Total RNA was extracted using RNeasy Plus Universal Kit (73404, QIAGEN) following the manufacturer's protocol, including removal of genomic DNA. RNA samples were prepared sequencing at the sequencing facility, Frederick National Laboratory for Cancer Research, NCI, Frederick, MD. RNA libraries were prepared for sequencing using standard Illumina TruSeq Stranded mRNA library protocol.