GSM8187002: MED17-dTAG, clone2, dTAG, E2, H3K27ac; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde at room temperature for 10 min. Glycine was then added to quench cross-linking. Chromatin fragments were generated by sonication (Branson sonifier). ChIP-seq library was prepared with NEBNext Ultra™ II DNA Library Prep with Sample Purification Beads (New England Biolabs)