RNA-seq of Danio rerio wild type and kdm4b knockout mutant gata1+ erythroid progenitors and precursors
RNA-seq of Danio rerio wild type and kdm4b knockout mutant gata1+ erythroid progenitors and precursors
The 48 hpf gata1+ erythrocytes were sorted (FACSAria, BD Bioscience, CA, USA) Two independent groups of RNA from wt and kdm4b-/- erythrocytes were extracted using TRIzol® Reagent (Life Technologies) (each group contains three biological repeats). After adenylation of 3' ends of double strand cDNA fragments, Adaptor with hairpin loop structure were ligated to prepare for hybridization. The library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then PCR was performed with Phusion High-Fidelity DNA polymerase. At last, PCR products were purified and the library quality was assessed on the Agilent Bioanalyzer 2100 system.