GSM8098339: MeCP2, Arc guide, IP, rep2; Mus musculus; ChIP-Seq
20 million cells were crosslinked using 1% formaldehyde for 10 minutes at RT, followed by lysis in 5 mM HEPES (pH 8), 85 mM KCl, 0.5% NP-40. After centrifugation, nuclei were washed in 5 mM HEPES (pH 8), 85 mM KCl and then sonicated to 100–400 bp using 15 cycles 30” on-off in 1ml of 50 mM Tris-HCl (pH 8), 10 mM EDTA, 1% SDS using a Covaris LE220 sonicator. NEBNext Ultra II DNA Library Preparation Kit (NEB)