GSM8005659: Ami-YGL1_Line 7_field10.00.A.M._treatment_leaf_totalRNA_3; Oryza sativa Japonica Group; RNA-Seq
Trizol reagent (15596018, Thermo Fisher Scientific, USA) The p35s-Ami-YGL1 mutant plants were generated with RNAi technology as described earlier (Mao et al., 2023). We used three homozygote transgenic lines (line 1, 7 and 8) during this study.  Rice (Oryza sativa spp japonica cv Nipponbare) and p35s-Ami-YGL1 were grown in the experimental station of the Shanghai Institute of Plant Physiology and Ecology (121°8′1.33″E, 30°56′26.73″N). During the growth period in 2021 or 2022, the rice was transplanted into the well-fertilized field in a 7×7 layout (25cm ×25 cm) and for each line. To avoid boundary effects, plants belong to the boundary lines were excluded in the measurement. The experiments were done in two years at different field sites (2019, 2021).   At the same time, we also carried out relevant measurements in the plant greenhouse. The greenhouse was subjected to an alternating light/dark cycle (12 hours of light and 12 hours of darkness), with a light intensity of approximately 400 μmol m-2 s-1 and the temperature was set at around 28 oC.