DNA library 2 from head hair 2 of individual HP2-S068 (Hair2.0 panel): Sample H2Hair088-S068-H2
For each extraction, we prepared 3 Illumina libraries per extract for up to 2 head hair extracts and one pubic hair extract, depending on availability. We prepared Illumina libraries as described in Kapp et al. (2021) with the following modifications: 9 µL DNA extract, 2 µL 38 ng/µL ET SSB (NEB), 1 µL 2 µM P5 splinted adapter, 1 µL 0.4 µM P7 splinted adapter, and 12 µL reaction mix (per reaction: 9.20 µL 50% 8000, 1.25 µL 1M Tris-HCl, 0.25 µL 1M MgCl2, 0.25 µL 1M DTT, 0.25 µL 100mM ATP, 0.55 µL 10,000 U/mL T4 PNK (NEB), and 0.25 µL 2,000,000 U/mL T4 DNA Ligase(NEB)). Following ligation, we performed a SPRI clean as described in Rohland and Reich (2012) using 35 µL buffer EBT (10mM Tris, 0.5% Tween-20) and 60 µL SPRI solution. We eluted each library in 21 µL of buffer EBT. We performed qPCR and indexing PCR on each library as described in Kapp et al. (2021).