GSM8057964: week5 Transgen rep2; Mus musculus; RNA-Seq
Kidneys  were harvested from the mice and were subjected to RNA extraction using regular Trizol protocol For library preparation NEBNext RNA ultra II RNA library preparation kit (NEB) was used. All RNA samples underwent PolyA selection following the manufacturers' protocols. Samples were multiplexed using suitable molecular barcodes and resulting cDNA pools were processed according to the NextSeq System Denature and Dilute Libraries guide. (Illumina). Quantification and quality control of the libraries were done using Qubit fluorimeter and Agilent 4200 TapeStation.