GSM7727462: MuSC rep1, Pax7CreERT2/+::Amotflox/flox:: Rosa-YFP, 48 hrs; Mus musculus; RNA-Seq
Growth medium was removed and TriZol was directly added to the SCs. Direct-zol RNA Miniprep Kits (Zymo) were used for RNA purification. Total RNA was subjected to rRNA depletion using Ribo-Zero rRNA Removal Kit (illumina) and libraries were generated using the TruSeq RNA Library Prep Kit according to illumina manual.