Escherichia coli isolate from pig farm
Only ESBL-producing Escherichia coli isolates were selected for sequencing. Selection was based on phenotypic resistance pattern and origin of the isolate. DNA of isolates was extracted using the DNeasy UltraClean Microbial kit (Qiagen, Germany). DNA libraries were prepared using the Illumina DNA prep kit (manufacturers instructions), followed by 2 x 150 bp sequencing with P2 reagent.