Illumina NovaSeq 6000 paired end sequencing; Stromal protection against low dose AraC induced differentiation in U937 cells
Stromal protection against low dose AraC induced differentiation in U937 cells
U937 cells were treated with AraC (100 and 1000 nM) for 24h with or without the presence of MS-5 stromal cell line. After indicated time, suspension cells were collected, washed in PBS and RNA was extracted. RNA was extracted using TRyzol-based extraction procedure. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library