GSM7421502: C57BL/6, 4 weeks, male, BAT, KO4; Mus musculus; RNA-Seq
RNA was harvested using RNeasy plus mini kit (Qiagen). 1.5 µg of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.Libraries were sequenced using Hiseq3000 platform to a depth of approximately 20 million reads per library with single-end read 50 base pairs read length.