GSM6380208: Group 3 innate lymphoid cell, Cxxc1-deficient  ILC3s rep2, scRNAseq 1; Mus musculus; RNA-Seq
Small intestinal ILC3s were sorted from siLP of Cxxc1flox/floxRorccre mice. All samples were uniformly resuscitated and washed twice by PBS (with 0.1% BSA), then the cells were 1 ml stain buffer (with 0.1% BSA) and counted. The viability of cells in all samples was assessed >80%. Cells for all samples were labeled with sample tags (BD Mouse Single-Cell Multiplexing Kit, USA), counted, and multiplexed, ready for single-cell capture. Single-cell capture and cDNA synthesis were performed by the BD Rhapsody Single-Cell Analysis System (BD, USA), according to the manufacturer's instructions.