GSM6598617: Hep3B_siRNA3_10nM_rep1; Homo sapiens; RNA-Seq
Dynabeads Oligo (dT)25 (Thermo Fisher Scientific 61005) Whole transcriptome mRNA-enriched RNA-Seq libraries were constructed using a KAPA mRNA Capture Kit and mRNA HyperPrep Kit (Roche) from cell lysates as per protocol but in 5x miniaturized reagent scale (except mRNA capture beads were resuspended in water prior to mix with lysate). RNA-Seq libraries were quantified by low depth sequencing on Illumina iSeq instrument . Equal amounts of each library/sample were pooled and sequenced on a Illumina NovaSeq instrument with 2x150bp paired-end settings, according to manufacturers' instructions.