GSM6734316: WT ES cells (HM1) day 2 biol rep 2  technical replicate c; Mus musculus; RNA-Seq
RNA extraction was performed using NucleoSpin RNAII isolation kit (Macherey-Nagel) Library preparation and Illumina sequencing were performed at the Ecole normale superieure genomics core facility (Paris, France). Messenger (polyA+) RNAs were purified from 1 µg of total RNA using oligo(dT). Libraries were prepared using the strand specific RNA-Seq library preparation TruSeq Stranded mRNA kit (Illumina).