GSM8186646: BMDM,UT; Mus musculus; ATAC-seq
Cells were trypsinized with 0.25% trypsin-EDTA and resuspended in 1X PBS (calcium and magnesium-free). For the ATAC-seq library preparation, 5×104 viable cells were used and collected. The cell pellets were resuspended in cold lysis buffer (1 M Tris-HCl, pH 7.4, 5 M NaCl, 1 M MgCl2, IGEPALCA-630) and incubated on ice for 5 min. Then, the cells were collected and resuspended with 50 μl transposition mixtures according to ATAC Kit instructions (Vazyme, #TD501). Mixtures were incubated for the PCR reaction. Mixtures were purified with VAHTS DNA Clean Beads (Vazyme, #N411) and amplified for 15 cycles using TruePrep Index Kit V2 for Illumina (Vazyme #TD202). Amplification products were further purified with VAHTS DNA Clean Beads. Mixtures were incubated for the PCR reaction. Mixtures were purified with VAHTS DNA Clean Beads (Vazyme, #N411) and amplified for 15 cycles using TruePrep Index Kit V2 for Illumina (Vazyme #TD202).