GSM7813925: EXOSC2 AID ESCs, RNA, 0 hr, RAD0, rep2; Mus musculus; RNA-Seq
Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to poly(A) purification by VAHTS mRNA Capture Beads (Vazyme) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols.