GSM8097108: Nox2 Infected Neutrophil Replicate 3; Mus musculus; RNA-Seq
RNA was isolated from WT and NOX2 KO neutrophils (n = 3 replicates/group) using the MirVanaTM ParisTM kit (AM1556, ThermoFisher Scientific). 0.5ug were used for the construction of sequencing libraries. Total RNA was ribo-depleted using NEBNext Mouse ribodepletion kit from 100-1000ng input material, and NEBNext UltraII Directional RNA library prep kit to generate the library.