A high-resolution single-cell multiomic atlas of the human fetal lung - scRNA / GEX / VDJ
A high-resolution single-cell multiomic atlas of the human fetal lung - scRNA / GEX / VDJ
Protocol carried out by HDBR to extract specimen from a fetal donor.  Nucleic acid was extracted according to the standard protocol of the Chromium single cell 5'V1 kit. Libraries were prepared according to the standard protocol of the Chromium 5'V1 VDJ kit. An in-house kit was used for gdTCR enrichment.  V(D)J libraries were prepared according to the manufacturerâ€™s protocol (10X Genomics) using individual Chromium i7 Sample Indices.5,000 PE reads per cell for V(D)J libraries were aimed for sequencing. Libraries for gamma/delta TCR variable regions were amplified as previously described (Mimitou et al. 2019)