GSM6387679: B22_10010144; Homo sapiens; RNA-Seq
DNA and RNA was extracted using the Maxwell® RSC Instrument and appropriate kits (Promega) from all tissues and quantitated using a Qubit Fluorometer. DNA libraries were prepared at BGI from 10ng input genomic DNA (representing roughly 2000 nuclei) from each tissue using the NEB kit (NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina (https://www.neb.com/products/e7805-nebnext-ultra-ii-fs-dna-library-prep-kit-for-illumina#Product%20Information). Samples were sequenced on the BGISEQ500 by 100bp paired end. RNA-seq was performed at BGI using their Eukaryotic Transcriptome Library Construction Protocol, an RNase H method for library prep, and libraries were sequenced on HiseqXten by 150bp paired end.