Viruses associated with healthy and bleached thalli of the green seaweed Ulva
Sample was homogenzied, centrifuged, filtered on a 0.8um filter, and treated with benzonase and micrococcal nuclease. DNA and RNA were extracted with the QiaAmp Viral RNA Mini kit (QIAGEN). Nucleic acids were randomly amplified with a modified whole-transcriptome amplification 2 (WTA2) kit procedure (Sigma-Aldrich). The amplified products were purified using the MSBSpin PCRapace purification kit (INVITEK).