GSM6697944: X267470_4_ACTTGA_L001; Homo sapiens; miRNA-Seq
Total RNA was extracted using Trizol Reagent for cell lysate or Trizol LS (ThermoFisher Scientific) for EVs and sera following manufacturer's instructions.  RNA was extracted from ~0.5 mL of serum and the final RNA pellet was dissolved in 10 μL of RNase-free water and subjected to small RNA-seq. Turseq small RNA library stragety with some modificatons: 1) add "NNN" at 3' of 5' adatpor, and "TCT" at 5' of 3' adaptor; 2) add RT primer after 3' adaptor ligtation to block free 3' adptors