Bromodomain-containing protein BRPF1 as a therapeutic target for metastatic ovarian cancer
Bromodomain-containing protein BRPF1 as a therapeutic target for metastatic ovarian cancer
cells were collected using standard protocols (trypsin). standard protocol standard protocols for cell lines cells where treated with compound for certain number of hours.  standard protocol Total RNA was extracted using TRIzol from PEO4 and OVCAR-3 cells treated for 72 hours with 10uM GSK6853 or DMSO as a control. In case of BRPF1 silencing experiment, RNA extraction was performed from cells transfected with BRPF-targeting siRNA or nontarget (scramble) siRNA 96 hours post-transfection using TRIzol and Zymo RNA Clean & Concentrator-5 columns.  Indexed libraries were prepared starting from 1000 ng of total RNA according to Illumina Stranded Total RNA prep Ligation with Ribo-Zero Plus kit. Final libraries were sequenced at a concentration of 0,6 nM/lane on the Illumina Novaseq 6000 using S4 flowcell and v1.5 reagents.