ONT Sequencing of human gastric cancer tissue: peritumoral tissues
The library was prepared as recommended by Nanopore (SQK-LSK109, Oxford Nanopore Technologies, ONT) and loaded onto a GridION5 flow cell (FLO-MIN106 R9.4.1, Oxford Nanopore Technologies) for a 583-hour sequencing protocol using MinKNOW software at Rhonin BioSciences Company (Chengdu, China)