PACBIO sequencing of Strogylocentrotus purpuratus BAC library: BAC 44
Library strategy and selection: BACs were created for the sperm of a single S. purpuratus using restriction digest and cloning into a vector. BACs were screened for the presence of SpTrf genes using southern blot and PCR amplification of the SpTrf genes. BACs that were positive were sent for sequencing.