RNA-seq of human bone marrow aspriate: Established myelodysplastic CMML
Total RNA was extracted using the MagNA Pure 96 Instrument and the MagNA Pure 96 Cellular RNA LV Kit (Roche LifeScience, Mannheim, Germany). The TruSeq Total Stranded RNA kit was used, starting with 250ng of total RNA, to generate RNA libraries following the manufacturers recommendations (Illumina, San Diego, CA, USA).