GSM6637118: ME (APS); OCT4KD ; Lib343; Homo sapiens; RNA-Seq
1 million cultured cells were dissociated to single cells with Accutase and were mixed with 100,000 of Drosophila Schneider 2 (S2) cells for a spike-in control. Cells were pelleted and lysed with Aurum Total RNA Lysis Solution (BioRad # 7326802). Total RNA was extracted by Aurum™ Total RNA Mini Kit (BioRad #7326820) and sent to Novogene for library preparation and paired-end-150bp sequencing on an Illumina NovaSeq 6000. Novogene performed the library construction