GSM7498516: Cd4cre-2; Mus musculus; RNA-Seq
CD8+ T cells were collected from spleen of Cd4cre or Cd4creGlut10fl/fl mice, flash frozen on dry ice, and RNA was harvested using Trizol reagent. A total amount of 2 μg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (#E7530L, NEB, USA) RNA libraries were prepared for sequencing using standard Illumina protocols