GSM8239779: Ctrl_+GFP, replicate1, scRNA-seq; Mus musculus; RNA-Seq
Fresh hippocampus was removed with tweezers and then dissociated into single cell suspension using the papain kit (Worthington) according to manufacturer's protocol. The cell suspension was injected into the SCOPE-chip™ microfluidic chip to complete the separation of single cells based on the principle of "Poisson distribution". Cells fell into specially customized chip microwells under the influence of gravity, ensuring that only one cell fell into each microwell. The magnetic beads in the chip were collected, and the mRNA captured by the magnetic beads was reverse transcribed into cDNA and amplified. After cDNA fragmentation, adapter ligation and other necessary steps, a sequencing library suitable for the Illumina sequencing platform was constructed.