GSM8239731: Chd4 Ctl ATAC rep2; Mus musculus; ATAC-seq
mechanical trituration - P0 dissected murine retina For ATAC-seq, we followed Buenrostro et al. [54]. Briefly, 75,000 cells were sorted from Chx10-Cre-YFP animals using YFP to purify RPCs. Cells were lysed in cold lysis buffer (10 mM Tris-Cl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, and 0.1% IGEPAL CA-630). Lysed nuclei were tagmented using 6.5 µl of TDE1 transposase from the Nextera DNA Flex Library kit (Illumina). Samples were purified using Zymo-Spin IC columns (Zymo), and libraries constructed according to the Nextera workflow. Libraries were cleaned up using the AMPure XP kit (Beckman Coulter). PE 150 sequencing was performed using the NextSeq 500 platform.