GSM8097127: SIGNAL-seq RNA library of HeLa spheroid cells; Homo sapiens; OTHER
Spheroids were fixed in-situ using an adapted SPLiT-seq protocol (Rosenberg and Rocco et al., 2018) and dispersed by mechanical dissociation using a TissueGrinder. Single-cells were permeablilsed using an adapted SPLiT-seq protocol and frozen before processing with SIGNAL-seq. scRNA-seq RNA and ADT libraries were generated using a modified combinatorial barcoding (Rosenberg and Rocco et al., 2018) SIGNAL-seq protocol producing two sequencing libraries (RNA and ADT) for each sublibrary. SIGNAL-seq