GSM8058162: Human ETMR tumor, C19MC amplified, 5; Homo sapiens; RNA-Seq
Sample preparation for deparafinization and isolation of cells from FFPE tissue sections following 10x Genomics for Fixed RNA seq - protocol CGO000632_RevB, using gentleMACS Octo dissociator with heaters (Miltenyi, Cat n. 140-004-386.06). Protocol for probe hybridization, GEM generation and barcoding, amplification and library construction as per 10x Genomics Fixed RNA seq protocol CG000527_RevE, for multiplexed library (16 rxns x 16 BC): 1Mio cells per sample taken for probe hibridization; strategy washing -> pooling for pool preparation; targeted 80.000 cells per library. Chromium Fixed RNA Profiling for Multiplexed Samples