GSM7502290: acuteCVS269; Mus musculus; MeDIP-Seq
Qiagen, Dneasy Blood & tissue Kit 500 ng of DNA were shared by ultrasound to an average length of 200 nt (Bioruptor® Pico; Diagenode SA, Seraing, Belgium). DNA was then processed in two steps and subsequently analyzed by Next-Generation Sequencing. Sample complexity was reduced by enrichment of DNA fragments with methylated cytosines using the EpiXplore™ Methylated DNA Enrichment Kit (Takara Bio Inc., Japan).  NEB Ultra-II Library kit (NewEngland BioLabs® Inc.) was used according to manufacturer's instructions. Libraries were paired-end sequenced on the NextSeq550™ instrument with automated FastQ raw data analyses (Illumina, San Diego, USA).