GSM6944068: PRO_dTAG-3h_INTS8-dTAG_vector_DLD1; Homo sapiens; OTHER
RNA was isolated with TRIzol. Enriched RNA was ligated with RNA adapters. Reverse transcription (RT) was performed using the Superscript III enzyme (Invitrogen). The reverse transcription products were proceeded to library amplification by using the Q5 enzyme mix.  Libraries were sequenced on the Illumina NovaSeq 6000 following the manufacturer's protocols. PRO-Seq