RNA-Seq of mus musculus cholangiocytes
After co-culture, live T cells (DCM-CFSE-CD3+) and large cholangiocytes (DCM-CFSE+) were purified by FACS Aria II (BD Biosciences), snap-frozen, and delivered to Novogene (Cambridge, UK) for RNA isolation and sequencing. All samples had 2-3 biological replicates and sequenced by the NovaSeq 6000 system.