GSM6459726: Capture Hi-C, NA07056-batch-2, biological replicate A; Homo sapiens; OTHER
Capture Hi-C libraries were prepared according to the protocol described in (Jäger et al., 2015: PMID: 25695508). The protocol consists of two parts: Hi-C library preparation and target enrichment (Figure 1A). A SureSelect Custom Target Enrichment Library covering a 3Mb region in the 8p23.1 (hg19 coordinates: chr8:8190000-11838000) was designed using eArray software (Agilent). Hi-C library preparation, comprising chromatin fixation, HindIII digestion, biotin labelling, ligation and crosslink reversal was performed as described in (Rao et al., 2014: PMID: 25497547) with minor modifications described in (Jäger et al., 2015). Target enrichment was performed according to the SureSelect protocol (Agilent) with minor modifications described in (Jäger et al., 2015: PMID: 25695508). We have prepared 10 capture Hi-C libraries from 5 independent batches of NA07000 cells and 4 capture Hi-C libraries from 2 independent batches of NA07056 cells (2 biological replicates per cell batch). Capture Hi-C