Genomic sequencing of SARS-CoV-2: Wastewater, USA:North Carolina
Viral sequencing was performed following a tiling amplicon strategy using the V4.1 primer scheme. Sequencing was performed using an Illumina MiSeq sequencer. Libraries were prepared using SuperScript IV Reverse Transcriptase, Q5 High-Fidelity DNA Polymerase pack by NEB, and Miseq Reagent Kit v3.