GSM8283728: MCF7-EM cells, ethanol control, 24hrs, biorep1; Homo sapiens; RNA-Seq
Total RNA was extracted from MCF7-EM and MCF7-ER cells treated with vehicle control or 10 nM E2 for 24 hours using the NucleoSpin RNA extraction kit, which included a DNase digestion step to remove potential contaminating DNA (Macherey-Negel). cDNA libraries were made from 1 µg total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490) and NEBNext Ultra Directional RNA Library Prep Kit for Illumina (E7420) and indexed for sequencing using NEBNext Multiplex Oligos for Illumina.