GSM6460056: HAP1, Untreated, Pbrm1, Bethyl; Homo sapiens; ChIP-Seq
Chromatin immunoprecipitation was performed as described (Miller EL et al. Nat Struct Mol Biol. 2017;24:344–52). Briefly, 10-30 million cells were cross-linked with 1% formaldehyde for 12 mins for room temperature, followed by  quenching with 125 mM glycine for 10 mins, washed with PBS, pelleted and flash-frozen. Nuclei were isolated, rinsed and sonicated using an E220 Covaris sonicator, and the supernatant was used for immunoprecipitation with the indicated antibody. Antibodies used were anti-Pbrm1 (Bethyl, catalogue no. A301-590A and Novus Biologicals, catalogue no. NB100-79832). ChIP-sequencing libraries were prepared using Swift 2S ligation kit according to standard protocols and sequenced to a minimum of 40 million 150 bp read pairs each sample. ChIP-seq libraries were prepared using Swift 2S ligation kit according to standard protocols.