WGS A. fumigatus
Sequencing libraries were generated using on-bead tagmentation chemistry with the llumina DNA Prep, (M) Tagmentation Kit (Illumina, Inc.) according to the manufacturers protocol. Briefly, bead-linked transposomes were used to mediate the simultaneous fragmentation of gDNA (100-500ng) and the addition of Illumina sequencing primers. Next, reduced-cycle PCR amplification was used to amplify sequencing ready DNA fragments and to add the indices and adapters. Finally, sequencing-ready fragments were washed and pooled prior to paired-end sequencing (151 + 151 cycles, plus indices) on an Illumina NextSeq500 instrument