GSM6735425: ChIP-seq_H3K27ac_CD4T_control; Mus musculus; ChIP-Seq
Lungs were cut into small pieces and digested in RPMI containing collagenase A and Dnase I using gentleMACS Dissociator and then passed through 70 µm filter. Lung ILC2s and CD4 T cells were sorted by SONY SH800 following crosslink with 1% formaldehyde. Sonicated samples were immunoprecipitated using H3K27ac antibody (ab4729, Abcam) and Dynabeads Protein A. Chromatin-DNA complexes were washed seven times and reverse-croslinked by overnight incubation at 65 ˚C. DNA fragments were harvested with phenol-chloroform extraction followed by ethanol precipitation. ChIP-seq libraries were prepared with ThruPLEX DNA-seq kit.