GSM6637201: R2; Homo sapiens; RNA-Seq
Xenograft tumors were then snap-frozen in liquid nitrogen and stored at -80°C prior to shipment on dry ice to GENEWIZ (South Plainfield, NJ) for sequencing. Libraries were prepared using the Tecan Ovation SoLo RNA-Seq library preparation kit per manufacturer's instructions. Libraries were quantified using Quant-iT PicoGreen dsDNA assay kit and read on BioTek FLx800 fluorescence microplate reader. Libraries were pooled equal molar and the sample pool was quantified using Biorad ddPCR. Libraries were sequenced using as stated below. A Single-cell and Bulk-RNA sequencing dataset generated by Genewiz, Inc (South Plainfield, New Jersey). The Bulk-RNA sequencing dataset comprises six samples, of which three have normal expressions of SLFN12, and the remaining three have over-expression of SLFN12. Similarly, single-cell sequencing consists of two samples, of which one has normal expression, and the other has over-expression of SLFN12. After receiving the raw files (FASTQ) from Genewiz, pre-processing and normalization was performed in-house using the following approach.