GSM6631805: VD1_W17; Citrus sinensis; RNA-Seq
Total RNA was extracted using TRIzol® Reagent and purified using an RNeasy Mini Kit following the manufacturer's protocol Ribosomal RNA was removed from the total RNA using a Ribo-Zero rRNA removal kit  for using plant seed/plant leaf following the manufacturer's protocol. Subsequently, 50 ng of the purified RNA from each sample were used for library construction utilizing an Epicentre ScriptSeq v2 RNA-Seq library preparation kit according to the user's guide.