GSM7877401: HEK293T DUS1L KO 1-9 tRNA library_replicate 3; Homo sapiens; ncRNA-Seq
Total RNA was extracted from cells using TRIzol reagent following the manufacturer's protocol. Transfer RNA was isolated from total RNA by gel extraction. RNA-seq libraries were prepared using the NEBNext® Small RNA Library Prep Set for Illumina® (NEB #E7330S) following the manufacturer's protocols.