16S of potato leaf
The concentration of PCR products was checked on Agilent 2100 Bioanalyzer system. Volumes constituting 30 ng of DNA of each sample were pooled to prepare an equimolar library. Library samples were size-fractionated on Pippin Prep (Sage Science, MA, USA) using 2% Agarose gel cassette (Marker B) selecting 350-500bp amplicons. The purified libraries were sequenced on Ion 314 Chip v2 in Ion Personal Genome Machine (PGM) (ThermoFisher Scientific, USA).