RNASeq of combined triplicate Mycobacterium ulcerans moved from control to Temp 37C and aerobic for 24H
RNA libraries were created from combined triplicate replicates of M. ulcerans RNA samples under aerobic and 300C (T0-T48H), aerobic and 370C (T24 H), microaerophilic and 370C (T24 H),  and samples that were transferred from 370C, aerobic or microaerophilic conditions to aerobic and 300C at 48 H [aerobic and 370C (T48 H), microaerophilic and 370C (T48 H)] conditions. Libraries were created using the NEBNext Ultra RNA Library Prep Kit and NEBNext Multiplex Oligos (Dual Index Primers) for Illumina and associated protocols. High-throughput RNA sequencing was performed by St. Jude Childrens Research Hospital on an Illumina HiSeq2000 with 2 X 150bp PE (paired end) read lengths.