GSM6460034: Delta3116_SM_N02_FKDL210115699-1a_L1_p1; Azoarcus olearius; RNA-Seq
The RNA was extracted from cells grown to OD578  0.4-0.5 by the hot phenol method described by Solaiyappan Mani, 2021. The rRNA was depleted by the NEBNext rRNA Depletion Kit (Bacteria) (NEB GmbH Brüningstraße 50, Frankfurt am Main, Germany, Product NO. E7850). The cDNA library of the enriched mRNA was prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB GmbH Brüningstraße 50, Frankfurt am Main, Germany, Product NO. E7760). All the steps were performed as described by the NEB protocols. The synthesized cDNA library was frozen directly in liquid nitrogen and then kept in -80oC freezer for long time storage.