GSM7416634: fGSOSS_rep4 (RNA-seq); Drosophila melanogaster; RNA-Seq
RNA was isolated using RNeasy Mini Kit (Cat. No. 74106, QIAGEN) with RNase-Free DNase Set (Cat. No. 79254, QIAGEN) for DNase digestion during RNA purification as per manufacturer's instruction. Reverse transcription was performed with 100 ng to 1 µg RNA using SuperScript™ IV Reverse Transcriptase (Cat. No. 18090010, Thermo Fisher Scientific). For total RNA-seq, ribosomal depletion was performed using riboPOOLs (siTOOLs Biotech) for Drosophila melanogaster (Cambridge Bioscience) using 500 ng of total RNA as input. For mRNA-seq, poly(A) selection was done using Poly(A) mRNA Magnetic Isolation Module (NEB # E7490) with 500 ng of total RNA as input. Libraries were prepared using The NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (#E7420L). Library quality was assessed using Agilent 2100 bioanalyzer (High Sensitivity DNA Kit).