ONTPS1
The DNA library was prepared by using ligation-based sequencing (SQK-LSK109) library preparation kit (Oxford Nanopore Technology, Oxford, United Kingdom) as per manufacturers guidelines. For ONT library construction, briefly the ends of high molecular weight intact DNA (1.0 g) wer repaired by using NEBNext End Repair/dA-tailing module. The end repaired DNA was purified using agencourt AMPure XP magnetic beads followed by adapters ligation to the purified DNA by NEBNext Quick T4 DNA ligase enzyme. The adapter ligated library, sequencing buffer and loading beads were mixed and loaded into the MinION flow cell (R9.4.1) and the sequencing was processed for 72 hours on MinION device