GSM8240727: Female-2; Aedes albopictus; ncRNA-Seq
Total RNA was isolated using TRIzol® reagent (Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer's instructions. The purity and concentration of the total RNA samples were determined using a 2100 Bioanalyzer (Agilent) and ND-2000 instrument (NanoDrop Technologies), respectively. Small RNAs were purified from isolated total RNA using 15% denaturing polyacrylamide gel electrophoresis (urea PAGE), and RNA oligonucleotide adaptors were sequentially ligated to the 3' and 5' ends of small RNA by T4 RNA ligase (Takara, Dalian, China).