Illumina HiSeq 4000 paired end sequencing; A gene regulatory network for neural induction
A gene regulatory network for neural induction
Samples were collected from prestreak epiblast dissected from EGKXII-XIII embryos and on mature neural plate dissected from HH6-7 embryos (15-20 pieces of tissue per sample). To prepare nuclei, we spun cells at 500g for 5 min, which was followed by a wash using 50 μL of cold 1× PBS and centrifugation at 500g for 5 min. Cells were lysed using cold lysis buffer.  Immediately after lysis, nuclei were spun at 500g for 10 min using a refrigerated centrifuge. Libraries were prepared exactly as described in (Corces et al., 2017), using the adapter sequences listed in (Buenrostro et al., 2013).