Illumina HiSeq 2000 paired end sequencing; A gene regulatory network for neural induction
A gene regulatory network for neural induction
Hensen's node was grafted into the area opaca of HH4 chick embryos and the epiblast in contact with the node was collected after 9 and 12 hours (9 and 12hrs induced); the contralateral area opaca from the same embryos was collected after 0, 9 and 12 hours (uninduced area opaca). Total RNA was extracted and the quality was assessed using the Agilent 2100 Bioanalyzer -all samples registered a RIN value between 9.0-10.0. Libraries were constructed using the IlluminaÂ® TruSeq mRNA library preparation kit.