Oxford Nanopore WGS for Neoseiulus californicus
The genome sequencing of the iso-female line was carried out on the material of genomic DNA from tick eggs. Since Neoseiulus californicus uses a parahaploid breeding system, the males of this species are haploid and the females are diploid. this feature was used to simplify the analysis of genome sequencing results. Isolation of total DNA from ticks or tick eggs for whole genome sequencing was carried out using the QIAGEN Genomic-tip 20/G kit (QIAGEN) which allows obtaining high molecular weight DNA. An assessment of the quality of DNA extraction, concentration will be carried out before the creation of libraries for sequencing. Whole genome sequencing was performed using a MinION nanopore sequencer, 512 channels (Oxford Nanopore Technologies). Sample preparation of total tick DNA is planned using the Ligation Sequencing kit MinION_Basic_Ligation SQK-LSK109. The process of sample preparation of the library for sequencing included the completion of DNA ends, ligation of the 3'-end dA, and ligation of adapters to it. Sequencing will be performed in FLO-MIN106D flow cells compatible with the MinION sequencer.