Co-targeting BET, CBP, and p300 inhibits neuroendocrine signalling in androgen receptor-null prostate cancer:MURAL PDX-435.1ACx RNA-Seq data of organoids treated with vehicle for 24hrs
Total RNA from PDXs, organoids and explants was isolated using RNAqueousTM-Micro Total RNA Isolation Kit (InvitrogenTM) with an in-build DNase-I treatment according to the manufacturer's instruction. Total RNA was quantified in a Nanodrop ND-1000 spectrophotometer, checked for purity and integrity in a Bioanalyzer-2100 device (Agilent Technologies). Libraries were prepared using Lexogen 3′ Quantseq sequenced using NextSeq550 HO with 75 bp single-end reads.