GSM6637222: ApoE3_LPS_scRNA_seq; Mus musculus; RNA-Seq
Brain tissues from 3 replicate mice per group were processed for 'glia-enriched' single cell suspensions (see Early et al., 2020). Sample libraries were constructed using Next GEM automated 3' reagents (10X Genomics, v3.1) following manufacturer's suggested protocol (#CG000286 Rev B). Final library quantification and quality check was performed using BioAnalyzer (Agilent), and sequencing performed on a NovaSeq 6000 S4 flow cell, 150 bp Paired-End sequencing (Novogene).