RNAseq of Acyrthosiphon pisum legs
Total RNA was extracted with the innuPREP RNA Mini Kit according to manufacturers instructions, eluted in 30 l RNAse free water, and sent to Novogene (Cambridge, UK) for RNA-seq library construction (polyA enrichment) and sequencing (NovaSeq PE150, paired reads, 6 gigabyte of raw data per sample).