RNA-seq of Breast Cancer Cell Line: MDA-MB-231
The total RNA was extracted from MDA-MB-231 cells using TRIzol reagent (Takara, Dalian, China) and further purified by rRNA depletion, followed by cDNA synthesis and RNA amplification, according to the manufacturers instructions. The RNA-seq libraries were constructed and sequenced utilizing the Illumina HiSeq2500 platform (Illumina, San Diego, USA).