GSM6735292: PAC-seq siRRP40 mESC Replicate 2; Mus musculus; OTHER
For SCR and U1 AMO conditions, libraries were constructed from 250 ng of enriched 4sU-labelled RNA (as described under TT-seq extraction protocol). For untreated mESC, siNT and siRRP40 conditions, 1 μg of total RNA (as described under RNA-seq extraction protocol) was used for library construction. PAC-seq libraries were constructed using the PolyA-ClickSeq Library Kit (ClickSeq Technologies)