UCE target enrichment of Dirrhope americana
Genomic DNA was extracted with the Quiagen DNeasy Blood and Tissue kit (Qiagen Inc.,Valencia, California, U.S.A.). DNA was sheared for 45 seconds in a Qsonica Q800R sonicator (Qsonica LLC, Newton, CT). Genomic libraries were prepared using the Kapa Hyper Prep Kit (Roche) and the custom Tru-Seq-style dual-indexing adapter (Glenn et al. 2016). ). Libraries were enriched with the Hymenoptera bait set 2.5Kv2 (Branstetter et al. 2017), which targets 2,590 UCE loci.