GSM6636864: PFC_WT/WT_M3; Mus musculus; RNA-Seq
RNA was isolated from HPC, PFC, (parietal) CTX, and CB from WwoxWT/WT and WwoxP47T/P47T mice, 150-280 days of age, n=5 mice/group (2 males, 3 females). Mice were euthanized, brains were extracted, and different tissue regions were collected on a cold plate and flash frozen in liquid N2. Total RNA was extracted using TRIzol reagent (Invitrogen) following standard manufacturer's protocol. RNA concentration and integrity were measured on an Agilent 2100 Bioanalyzer (Agilent Technologies). RNA samples with RNA integrity number (RIN) over 8.0 were considered for RNA sequencing. The samples were processed for mRNA-seq library construction using the ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicentre) according to the manufacturer's protocol. We performed 76 nt paired-end sequencing using an Illumina HiSeq3000 platform and obtained ~40 million tags per sample.