GSM7727692: Sample F4, Spared nerve injury, Knockout, Dorsal root ganglion; Mus musculus; RNA-Seq
RNA was extracted using the RNeasy Plus Universal Min Kit (QIAcube / QIAGEN) according to the manufacturer's instructions. Concentration and quality of extracted RNA were measured using the Qubit 4 Fluorometer (Thermo Fisher Scientific, MA, USA) and the 2100 Bioanalyzer Instrument (Agilent Technologies, CA, USA) Libraries from mRNA were prepared using 1 μg of RNA starting material and the TruSeq Stranded mRNA Library Prep Kit (Illumina, CA, USA) according to the manufacturer's protocol