Hibiscus liliiflorus Cav.
Approx. 500 ng genomic DNA fragmented with type-IIB enzyme BcgI (New England Biolabs), producing 36 bp fragment. Illumina adapters ligated on both ends, then Illumina primers and 6 bp Illumina TruSeq barcodes. Fragments amplified with 15 PCR cycles. PCR band cut out, then purified with MinElute gel purification kit (Qiagen). Purified DNA quantified then pooled in equimolar ratios. Sequenced on Illumina HiSeq 4000 using 50 bp single-end reads at NUSeq Core, Northwestern University. Raw reads demultiplexed with custom script, then quality filtered with FASTX-Toolkit 0.0.14 with parameters: -Q 33 -q 20 -p 90.