RNA-Seq of Micropterus Salmoides: adult liver
The RNA in liver were extracted, and then RNA purity, integrity and concentration were measured. Poly(A)-mRNA was purified from mixed RNA using poly-T oligo-attached magnetic beads and then randomly broken into short fragments by adding fragmentation buffer. The cDNA was then processed with end repair, poly(A) addition, and sequencing adaptors ligation, followed by fragment size selection and PCR enrichment. Finally, the cDNA library was sequenced using Illumina HiSeqTM sequencing platform.