GSM6636451: CVS_71074_Male_NIFT; Homo sapiens; RNA-Seq
Chorionic villi tissue was homogenized in RLT Plus lysis buffer (QIAGEN) containing 1% beta-mercaptoethanol, physically dissociated with single use needles attached to a 1 mL syringe. Total RNA was isolated from the homogenate using the AllPrep DNA/RNA Mini Kit (QIAGEN) following the manufacturer protocol. RNA-seq libraries of >200 nt RNA were constructed from 200 ng total RNA per sample using Illumina TruSeq Stranded Total RNA with Ribo-Zero Gold sample prep kits (Illumina). Libraries were depleted of cytoplasmic and mitochondrial ribosomal RNAs.