GSM7412620: CD8+ TIls, cKO ctrl; Mus musculus; ATAC-seq
Subcutaneous MC38 tumors were minced by razors, dissociated by using gentle MACS dissociator (Miltenyi Biotec) to single cell suspension, and passed through a 70 μm mesh nylon strainer, from which CD8+ TILs were sorted using the BD FACS ARIA II SORP (BD Biosciences) and resuspended in 1 mL PBS. Cells were resuspended in the transposition reaction mixture (Nextera DNA Library Preparation Kit, Illumina) and incubated at 37℃ for 30 min. After transposition, DNA was immediately purified with the MinElute PCR purification kit (Qiagen). To amplify transposable DNA fragments, barcode PCR primers were used for PCR. Then the amplification library was purified with the MinElute PCR purification kit and sequenced by Illumina NovaSeq 6000 (75 bp; paired-end).