GSM7879583: 1 day 10 HA T Donor 2; Homo sapiens; RNA-Seq
Total RNA was extracted from approximately 5E6 primary human T cells on day 10 of culture using the RNEasy Plus kit (QIAGEN) according to the manufacturer's protocol using additional 2-mercaptoethanol. Total RNA was sent to Novogene (Davis, CA) for cDNA library preparation for mRNA sequencing (polyA capture). 150bp paired-end read (Illumina PE150).