RNAseq of Staphylococcus aureus: SLM OD600 0.2-0.3 replicate 2
RNA was purified using the Qiagen RNeasy Plus Mini Kit. Samples were DNAse treated with Invitrogen DNAse (RNAse free). Library preparation was performed using Illumina's Stranded Total RNA Prep Ligation with Ribo-Zero Plus kit and 10bp IDT for Illumina indices. Sequencing was done on a NextSeq2000 giving 2x51bp reads. Demultiplexing, quality control, and adapter trimming was performed with bcl-convert (v3.9.3)