GSM7813938: EXOSC2 AID ESCs, RNA, RNPmesh, rep1, 4 hr; Mus musculus; RNA-Seq
ESC nuclei were subjected to DNase I treatment followed by sequential washes using detergents (1 M urea and 1% NP40) and high-salt (2 M NaCl). The resulting pellet, representing the 'insoluble' RNP mesh, was used for RNA-seq. Add the RNA spike-in (drosophila S2 cells) directly to samples according to number of cells. Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to poly(A) purification by VAHTS mRNA Capture Beads (Vazyme) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols.