GSM6398796: ADS patients_3; Homo sapiens; RNA-Seq
According to the manufacturer's instructions, the total RNA was extracted from each WB sample employing TRIzol reagent (Invitrogen, Carlsbad, CA, United States).The temperature was maintained at −80°C, while the RNA quality was measured by the Agilent 2,100 TapeStation (Agilent Technologies, United States) Biochip Analysis System. Eventually, samples were selected with RNA integrity values > 7.0 to construct cDNA libraries. The Illumina® NEBNext® Ultra™ Directed RNA Library  Preparation Kit is utilized to construct Illumina True-Seq  strand lncRNA libraries.