Illumina NovaSeq 6000 paired end sequencing; RNA-seq of mouse BMDCs treated with compound #634 or vehicle controls
RNA-seq of mouse BMDCs treated with compound #634 or vehicle controls
BMDCs collected from a 79-day-old female mouse were cultured with 5 uM compound #634, or 0.1% DMSO. 5 h later the cells were lysed in RNA lysis Buffer for RNA extraction. Total RNA was isolated using the Quick-RNA Miniprep Kit (Zymo Research, R1055) according to the manufacturer's instructions. Biological triplicates were obtained for each genotype.  1 ug of each sample's total RNA was then prepared into barcoded mRNA libraries, according to manufacturer's instructions, using the Illumina® Stranded mRNA Prep kit (Illumina, #20040534).