GSM8058153: TCTN2_dko; Homo sapiens; RNA-Seq
To generate neural progenitor cell fate, the following reagents were used during specific time periods: From day 0 to day 6, a combination of 50 ng/ml Noggin and 10 μM SB431542 was employed. From day 4 to day 8, 3 μM CHIR99021 was added. Additionally, from day 4 to day 11, 0.1 μM RA was introduced to drive the neural progenitor cells towards a caudal fate. To induce the expression of BMP4 and SHH proteins, which are generated from each organizer center, 200 ng/ml DOX was added from day 7 to day 11. After the aforementioned steps, dorso-ventral patterned neural tube organoids were generated. Libraries were prepared using Single Cell 3′Library & gel Bead kit v3.1 (10x Genomics, Cat# PN-1000121) according to the manufacturer's protocol for 10000 cells recovery.