GSM7814613: [Liver] [Ethanol-fed + PCB 126] [Replicate 1]; Mus musculus; RNA-Seq
Total RNA was extracted using the standard RNA-STAT60 and chloroform method. Samples were then treated with DNase, Amplification Grade I to digest single- and double-stranded DNA species. Finally, RNA samples were treated with RNAClean XP beads at a 1:1 volume ratio to further purify our samples to remove salts, dNTPs, and miscellaneous enzymes The TruSeq Stranded mRNA Library Prep Kit (Illumina) was used for library construction