GSM8008992: Sirt2 KO vehicle replicate A; Mus musculus; RNA-Seq
Islets were isolated using standard protocols with the inclusion of 10% FBS in place of BSA into HBSS solutions post-digestion. Islets were pooled from 2-3 mice per group, dissociated with 0.05% trypsin, and stained using propidium iodide for subsequent FACS sorting for live (PI-negative) cells. 3500 cells per sample were loaded in duplicate for each group and processed according to the manufacturer's instructions using 10x Single Cell 3' v2. According to the manufacturer's instructions using 10x Genomics Single Cell 3' v2. According to the manufacturer's instructions 10x Genomics Single Cell Library Contruction Kit