GSM8097181: Nr2e3_KO_replicate_1; Mus musculus; RNA-Seq
6-months post-injection the retinas of injected mice were dissociated with papain and single cell suspensions were FAC sorted based on mCherry expression from the AAV. Approximately 100,000 cells were sorted per sample, each of which consisted of two injected retinas from a single animal. Total RNA was isolated in Trizol according to manufacturer's instructions RNA integrity was determined using a Bioanalyzer. Total RNA was transcribed into cDNA using the SMARTer Ultra Low RNA kit for Illumina Sequencing per manufacturer's instructions.