GSM6734713: spinal cord-syringomyelia 4w-6w, replicate 3 [scRNA-seq]; Rattus norvegicus; RNA-Seq
The purity of RNA was determined by NanoPhotometer (IMPLEN, CA, USA). RNA integrity and concentration was assessed using the RNA Nano6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Qualified RNA was subjected to construct double stranded PCR library using MGI Easy RNA library preparation kit. Sequencing was performed on DNBSEQ-T7 and 150bp double ended sequencing reads was obtained. The volume of single-cell suspension that was required to generate single-cell GEMs (gel beads in the emulsion) per sample was loaded onto the Chromium Controller (DNBSEQ-T7). Libraries were prepared using the DNBelab C series single-cell RNA library preparation kit according to the manufacturer's specifications. Final library quantification and quality control were performed using a Qubit 3.0 and LabChip GX Touch, followed by sequencing on DNBSEQ-T7.