Pair-end sequencing of V. parahaemolyticus HS-180-10
DNA Prep: Frozen stock struck onto tryptic soy agar plates with 2% NaCl (TSA-2N) and incubated at 35 C overnight. An isolated colony was used to inoculate 5 mL tryptic soy broth with 2% NaCl (TSB-2N) at 35 C for 24 h, and DNA was extracted using the Zymo Quick-DNA HMW Magbead kit. NexteraXT library prep; paired-end (2x300 bp) sequencing using MiSeq and v3 chemistry.