GSM8009732: 27_T187_SARS-CoV-2 Alone; Homo sapiens; RNA-Seq
For scRNA-seq, bronchial AECs from a subset of the healthy nonsensitizedchildren (n 5 2) and children with allergic asthma (n 5 2) were differentiated ex vivo at an ALI to generate organotypic cultures by the same methods as already mentioned. Cultures were stimulated without or with IL-13 (10 ng/mL), with each medium change starting 1 week before and throughout SARS-CoV-2 infection Cells were loaded onto a 10X Genomics GemCode Single-Cell Instrument per manufacturer instructions for nanodroplet single-cell capture, cell lysis, mRNA rtPCR, and cDNA barcoding within droplets. NextSeq 10x chemistry