GSM6568463: SRSF2.P95L.Clone17; Homo sapiens; RNA-Seq
RNA was extracted from K562 cells using RNeasy Plus Mini Kit (Qiagen, Hilden, Germany). Samples SRSF2.P95R.Clone3.Batch2 and K562.Batch2 were extracted and sequenced as a different batch on different days than the rest of the cohort. Poly(A)-selected, unstranded Illumina sequencing libraries were prepared with the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA). 150 million 150 bp paired-end reads per sample were sequenced using the NovaSeq 6000 (Illumina San Diego, CA).