flower buds 1 of wild-type plants (replicate 3)
The goal of the RNA-Seq was to investigate the transcriptome changes induced by sus2 mutation in tomato flower development. Results of this RNA-Seq analysis provides valuable information for understanding the transcriptional regulation of flower development processes in tomato. Overall design: Three biological replicates from flowers at six different developmental stages, i.e.: flower bud 0 (FB0), flower bud 1 (FB1), flower bud 2 (FB2), flower at pre-anthesis (PA), flower at anthesis day (AD) and flower two days after anthesis (AD2), were sequenced for both wild-type and sus2 mutant plants. Tomato plants were grown under greenhouse conditions in natural sunlight (13-to 14h photoperiod). Daytime and night- time temperatures were 26-34 C and 20-25 C, respectively. Standard management practices were used including regular addition of fertilizers. Total RNA was isolated using TRIZOL (Invitrogen Life Technologies, San Diego, CA) following the manufacturer's instructions. Contaminating DNA was removed using the DNA-freeTM kit (Ambion, Austin, TX, USA). Libraries were prepared according to the Illumina TruSeq RNA protocol and sequenced with paired-end 150 bp on the Illumina HiSeq2000 platform (Illumina, Inc., San Diego, CA, USA).