V3V4 amplicon sequencing of bacterial 16S rRNA gene from human children: tonsillar crypt in recurrent tonsillitis
nucleic acids were extracted using the Qiagen AllPrep DNA/RNA Mini Kit then the V3V4 regions of the bacterial 16S gene amplified using the primers 341F-785R21A from Klindworth el al 2013. Amplicons were sequenced on the Illumina MiSeq 2x300bp