GSM8008717: Spontaneous tumor, 752OFF; Mus musculus; RNA-Seq
Tumors were dissociated into single cells using the Tumor Dissociation Kit from Miltenyi Biotec, followed by the removal of dead cells using the Dead Cell Removal Kit (Miltenyi Biotec). Cells were resuspended in PBS + 0.05% BSA at a concentration of 500-600 cells/μL for library preparation (10x Genomics). Library was constructed according to manufacturer's protocol.