GSM6637879: Bone marrow cells in treated with deoxycholic acid at 35uM for 1 week [BAT37Dd]; Mus musculus; RNA-Seq
Cells were isolated from our liquid co-culture system, as described above, and lineage-negative cells were FACS sorted to enrich for HSPCs prior to scRNA-seq. Cells were sorted into 1% FACS buffer into low-protein binding microcentrifuge tubes (Thermo Scientific). The cells were then spun down at 800g and resuspended in 50uL of PBS+0.04% BSA and pushed through a pipette tip-mounted flowmi filter (Millipore Sigma) to achieve a single cell suspension. The Genome Analysis and Technology core generated the single-cell indexed libraries and performed quality control checks to ensure high-quality RNA for downstream analysis.  Briefly, 8000 cells per sample were targeted for use with the 10X genomics chromium controller platform and Chromium single cell 3' library, and Gel Bead Kit v3.1 reagent. Generated cDNA was subject to quality control using the 4200 TapeStation instrument, using the D5000 Kit (Agilent).