Single cell RNA sequencing: mice pancreas
Single-cell capture, barcoding, and library preparation were performed using the Chromium 85 Controller, Chromium Next GEM Single Cell 3 GEM, Library & Gel Bead Kit v3.1, and the Chromium Next GEM Chip G kit (10xGenomics) with a cell recovery target of 100,000 cells as per manufacturers guidelines. cDNA and libraries were then sequenced on an Illumina NovaSeq 6000.