GSM7814177: CD11b+ cells, LPS4x_2; Mus musculus; RNA-Seq
Total RNA was extracted using an RNeasy Micro Kit (QIAGEN) The quality of RNA samples was assessed using an RNA 6000 Pico Kit (Agilent, Santa Clara, CA, USA) and 2100 Bioanalyzer (Agilent). 1 µg of total RNA was used for the construction of sequencing libraries. The sequencing libraries for RNA-seq were prepared using Hieff NGS™ Ultima Dual-mode mRNA Library Prep Kit according to manufacturer's protocol, and were sequenced with DNBSEQ-G400 (MGI) based on PE150 strategy.