GSM8098200: Aged,Ctrl,scRNA,rep3; Mus musculus; RNA-Seq
RNA was extracted according to manufacturer's protocol (Macherey-Nagel) and quantified immediately. RNA was then converted to cDNA using manufacturers' protocol (Bio Rad) The cDNA libraries were then completed in a single bulk reaction using the 10X Genomics Chromium NextGEM Single Cell 3' Reagent Kit version 3.1, and approximately 30,000 to 40,000  sequencing reads per cell were generated on the Illumina NovaSeq 6000 instrument using the S2-100 flow cell.  Demultiplexing, barcode processing, alignment, and gene counting were performed using the 10X Genomics CellRanger v6.1.2 software.