RNA-seq of cup1delta luc7delta upf1delta Saccharomyces cerevisiae complimented with luc7: WT-luc7 rep 1
40 ug of total RNAs extract was treated with DNAse I (Invitrogen, catalog #: 18-068-015) according to the manufacturers protocol. DNase I treated RNAs were ribodepleted using the RiboCop for Yeast kit (Lexogen, catalog #: 190.96). Libraries were prepared using the CORALL Total RNA-Seq V2 kit (Lexogen, catalog #: 175.96) following the standard protocol. Barcoding was done using the UDI 12 nt Set B1 (Lexogen, catalog #: UDI12B_0001-0096) and PCR amplification was done using 15 cycles. Sequencing was performed on NovaSeq PE150 by Novogene who oversaw demultiplexing.