DNA from EKW zebrafish individual sequencened at low coverage
Libraries were prepared using Twist 96-Plex Library Preparation kit (TWIST, San Francisco, CA), which is based on random priming. Libraries were prepared from 100 ng DNA in batches of 96 samples, then batches of 960 samples were loaded onto a single lane of Illumina NovaSeq 6000 and sequenced as PE150. Read were aligned to zebrafish genome assembly GRCz11 using BWA. Duplicates were marked using Picard MarkDuplicates function. Alignments were removed if their alignment quality score was 0.