RNA from Wild type S. flexneri  strain 2457T with Congo Red supplementation_R1
The NEB Next Ultra II RNA Library Prep Kit for Illumina (BioLabs, New England, MA, USA) was used for sequencing starting with the step of strand-specific library synthesis where the dTTPs were replaced by dUTPs during the synthesis of the second strand cDNA. The overhangs were converted to blunt ends, followed by adenylation of 3 ends and adapter ligation using RNA 5' and RNA 3' adapters included in the kit. Library concentration of 1.5 ng/ml was used after ensuring the insert size in a quality assessment prior to sequencing.