GSM7093293: SI epithelium of Tph2 KO 3; Mus musculus; RNA-Seq
Purified intestinal epithelial cells were lysed in Trizol (15596018, Invitrogen), and RNA was extracted with the RNeasy mini Kit (74106, Qiagen) according to the manufacturer's instructions. RNA-seq libraries were prepared using the KAPA mRNA HyperPrep kit (50-196-5308, Roche) or NEBNext Multiplex Oligos for Illumina kit (E7335L, NEB).