GSM7846851: KO; Mus musculus; RNA-Seq
Sorted splenic NK cells were washed twice with PBS and suspended in PBS supplemented with 400 μg/mL non-acetylated bovine serum albumin (Sigma Aldrich, B6917). Individual cells were then coupled to beads using the 10X Genomics Chromium controller (10X Genomics). Single-cell sequencing libraries were prepared following the 10X Genomics Protocol using 5' Reagent Kits v2 chemistry and sequenced to a median depth of approximately 20,000 reads per cell using an Illumina NovaSeq 6000 on rapid run.