Single-cell RNA-sequencing of CD138- immune cells from bone marrow or peripheral blood of healthy donors: Sample TCR83
Bone marrow or peripheral blood mononuclear cells were isolated using 1X red blood cell lysis buffer (Thermo Fisher). CD138- immune cells were magnetically sorted (Miltenyi Biotec) and the Chromium Single Cell 5' Gene Expression and V(D)J  enrichment kit by 10X Genomics was used for library preparation. TCR libraries were sequenced on a NovaSeq 6000 instrument at the Genomics Platform of the Broad Institute of MIT & Harvard.