Single-cell RNA-sequencing of CD138- immune cells from bone marrow or peripheral blood of patients with high-risk Smoldering Myeloma under treatment with Elotuzumab, Lenalidomide, Dexamethasone.: Sample GEX14
Bone marrow or peripheral blood mononuclear cells were isolated using 1X red blood cell lysis buffer (Thermo Fisher). CD138- immune cells were magnetically sorted (Miltenyi Biotec) and either the Chromium Single Cell 3' Gene Expression or the Chromium Single Cell 5' Gene Expression and V(D)J enrichment kit by 10X Genomics were used for library preparation. Libraries prepared with 3' technology were sequenced on a Hiseq 2500 instrument and libraries prepared with 5' technology were sequenced on a NovaSeq 6000 instrument at the Genomics Platform of the Broad Institute of MIT & Harvard.