WGS of human epilepsy patient
Whole-Genome libraries were prepared using NEBNext Ultra DNA Library Prep Kit (Cat. No: E7370L). Shearing of DNA was conducted to generate fragments of 350bp, followed by end repair, adenylation of 3 ends, andadapter ligation. At each step the products were purified using AMPure beads (Beckman Coulter, Cat. No: A63882). Adapter sequences were then added onto the ends of DNA fragments to generate paired-end libraries. The resulting adaptor-ligated libraries were purified and added index tags by amplification, followed by purification. Libraries were assessed to check the quality and quantity by using Agilent 2200 Tape station.