Gut metagenome raw sequence reads from Apis mellifera and Bombus spp.
Each sample PCR in duplicate with 515F, 939R primers using two-step library prep, adding in-house 8bp i5 and i7 indexes. Cleaned with Seramag Speedbeads. Each sample quantified using Qubit 3.0. Samples pooled equimolar. Sequenced on Illumina MiSeq.