GSM6598670: iBAT from cold exposed C57BL/6N ADCY3∆AT, HFD, replicate 1; Mus musculus; RNA-Seq
Tissues were homogenised and lysed in TRIZol using FastPrep24 homogenizer. Total RNA was isolated using EconoSpin All-In-One Mini Spin Columns (EconoSpin 1920-250). Paired end libraries were constructed using the NEBNext Ultra II RNA Library Prep Kit for Illumina following the manufacturer's protocol. Paired-end Total RNA