GSM8395646: Fat bodies, Control, rep2; Hermetia illucens; RNA-Seq
Four days after fungal treatment 5 larvae from each biological replication were dissected and the fat body was collected into Eppendorf on dry ice. RNA was then extracted using RNA extraction kit (Hybrid-RTM-GeneAll) and DNase removal performed using Clean-Up RNA concentrator Kit (A&A BIOTECHNOLOGY). The libraries were then constructed using ++NEBNext Ultra II RNA Library Prep Kit and NEBNext Poly(A) mRNA Magnetic Isolation Module