GSM8240900: human tumor cells from mice 106, tested group, ISRIB treament; Homo sapiens; RNA-Seq
RNA was harvested using NucleoSpin RNA isolation Kit (Macherey Nagel), by remplaced B-mercaptoethanol with DTT. Minimal of 1.0ug of total RNA was used for construction of sequencing librairies, except for samples m968-T for which only 0.658ug was used. The sequencing was performed by Macrogen, Inc. using the Novaseq 6000 RNA librairies for RNAseq were prepared using Truseq stranded mRNA kit following manufacturer's protocol.