GSM7727916: MT-Gal4 driven BonusKD RNA-seq biological replicate 3; Drosophila melanogaster; RNA-Seq
Total RNA was extracted from fly ovaries using TRIzol reagent, and ribosomal RNA was depleted with the Zymo-Seq RiboFree Total RNA Library Kit (Zymo Research, #R3000). RNA-seq libraries were made using the NEBNext Ultra II Directional RNA Library Prep kit for Illumina (NEB, #E7760) according to the manufacturer's instructions.