GSM7720006: Col WT Rep2 IP; Arabidopsis thaliana; OTHER
7days seedling were collected and total RNA was extracted by Trizol. mRNA were isoloated with Dynabeads™ Oligo (dT)25 beads and were fragmented into about 100-nucleotide-long fragments. N6-methyladenosine antibody were then used to do the MeRIP and the the libraries from immunoprecipitated RNA and input were constructed with the RNANEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB). Illumina RNA library protocol