GSM7093449: mMTHF4_raw_1; human feces metagenome; OTHER
Total genome DNA from samples was extracted using CTAB method. DNA concentration and purity was monitored on 1% agarose gels. According to the concentration, DNA was diluted to 1ng/µL using sterile water. 16S rRNA  genes of distinct regions(16S V3-V4 ) were amplified used specific primer( 341F(5'-CCTAYGGGRBGCASCAG-3')和806R (5'-GGACTACNNGGGTATCTAAT-3') )with the barcode. All PCR reactions were carried out with 15 µL of Phusion® High-Fidelity PCR Master Mix (New England Biolabs); 2 µ