Tetrangychuscinnabarinus_FeR01_good_1
The first strand of cDNA was synthesized using random hexamer primers. The second strand of cDNA was synthesized using RNase H and DNA polymerase I, and the cDNA was subsequently purified using magnetic beads (AMPure XP beads). Finally, the cDNA ends were repaired, and the cDNA was PCR amplified to construct the cDNA library.