GSM6380155: CD45 cells_CTR_2; Mus musculus; RNA-Seq
Cells were frozen in RLT buffer and subsequent RNA isolation was performed using Qiagen RNeasy Plus Micro Kit. SMARTer universal low input RNA kit (Takara Bio Inc) was used to convert RNA to cDNA. Bioanalyzer 2100 was used to measure cDNA quality and concentration. Nextera DNA Library Preparation Kit was used to make libraries Quality control of the libraries performed on an Agilent Technologies 2100 bioanalyzer using an Agilent high sensitivity chip. Multiplexed DNA libraries are normalized to 10nM and then pooled in equal volumes with each unique barcoded sample. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the HiSeq 4000 per the manufacturer's protocols.