GSM7937137: eWAT,high fat diet for 17 weeeks [CD44-3]; Mus musculus; RNA-Seq
Briefly, total RNA was extracted using Trizol reagent kit (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's protocol. After total RNA was extracted, eukaryotic mRNA was enriched by Oligo(dT) beads. The resulting enriched mRNA was fragmented into short fragments using fragmentation buffer and reversely transcribed into cDNA by using NEBNext Ultra RNA Library Prep Kit for Illumina The purified double-stranded cDNA fragments were end repaired, a base added, and ligated to Illumina sequencing adapters. The ligation reaction was purified with the AMPure XP Beads. Ligated fragments were then subjected to size selection by agarose gel electrophoresis and PCR amplification. The resulting cDNA library was sequenced using Illumina Novaseq6000 at Gene Denovo Biotechnology Co.