GSM6598114: cis_MEF; Mus musculus; Bisulfite-Seq
Genomic DNA from MEF cells was extracted using AllPrep DNA/RNA Mini Kit (QIAGEN).  500 ng genomic DNA  was used for bisulfite treatment and the construction of sequencing libraries. Sonicated, adaptor-ligated DNA was treated with sodium bisulfite by the EZ DNA Methylation-Direct kit (Zymo Research). The bisulfite modified DNA was amplified (18 cycles) using adaptor-specific primers and fragments of 200-500 bp were isolated. The quantity and size distribution of libraries were determined using the Pico Green fluorescence assay and the Agilent 2100 Bioanalyzer, respectively.