GSM7500866: MCF7 cells, palbociclib, rep1; Homo sapiens; RNA-Seq
RNA was iosolated using the NucleoSpin® RNA Kit (Macherey-Nagel, Düren, Germany). NGS library prep was performed by the core facility genomics of the IMB Mainz, with Illumina's Stranded mRNA Prep Ligation Kit following Stranded mRNA Prep Ligation, following the ReferenceGuide (June 2020). Libraries were prepared with a starting amount of 1000 ng and amplified in 9 PCR cycles.