The identity of the apoptotic cell explains the functional diversity of the efferocytic macrophages
The identity of the apoptotic cell explains the functional diversity of the efferocytic macrophages
Bone marrow-derived macrophages (BMDMs) were seeded into a 6-well plate at a concentration of 1.2·106 cells/well and exposed to apoptotic cells (apoptotic 6neutrophils (aN), apoptotic thymocytes (aT) and apoptotic hepatocytes (aH)) followed by IL-4 stimulation for 24h as described above (see polarisation assay). BMDMs were collected using cold PBS and 1 Mio cells were inoculated intravenously (i.v.) at week 6 and 7 post infection (p.i.). PBS was used as vehicle control. For the transfer experiments performed during thioglycollate-induced peritonitis, BMDMs were treated with the different apoptotic cells and IL-4, as described above, and transferred intra peritoneally (i.p.) 2 days after thioglycollate injection. PBS was used as vehicle control. BGISEQ-500 Transcriptome library was prepared according to the manufacturer's protocol. performed by BGI TECH SOLUTIONS, Hongkong