GSM6784417: KC_MR-7_CLAMP_Rep2; Drosophila melanogaster; OTHER
Cells were allowed to grow to confluency and harvested. Equal number of cells for each category suspended in wash buffer and subjected to CutnRun assay according to Skene and Heinkoff 2018. 1ng CutnRun DNA was used to generate libraries using Kapa Hyper prep kit and SeqCapAdapter Kit A. 14 PCR cycles used to amplify the libraries. Ampure Xp beads used for library purification and fragment analysis done to check quality of the libraries made. Paired end 2X25 bp illumina Hi-seq sequencing performed.