GSM6637440: HiC_ESC-rep1; Mus musculus; Hi-C
HiC was performed according to Arima HiC+ kit protocol (document part number: A160430 v00). After HiC reactions, the chromatins were sheared using Covaris S2 instrument with intensity setting 3 (equivalent to 105W), 5% duty cycle, and 200 cycles per burst for 7min. Then, sheared chromatins were decrosslinked for library preparation. Libraries were prepared according to Illumina's instructions with optimised rounds of PCR amplification, and finally analyzed the resulting fragments with paired-end sequencing.