WGS of X58540
The sequencing library is prepared by random fragmentation of the DNA sample, followed by 5 and 3 adapter ligation. This library is loaded into a flow cell where fragments are captured on a lawn of surface-bound oligos complementary to the library adapters. As all 4 reversible, terminator-bound dNTPs are persent during each sequencing cycle, natural competition minimizes incorporation bias and greatly reduces raw error rates compared to other technologies. Then, sequencing data is converted into raw data for the analysis.