GSM7498373: NC_1; Homo sapiens; RNA-Seq
Total RNAs were extracted from SHSY5Y cells using TRIzol Reagent (Invitrogen, Cat. No. 15596026) following the methods proposed by Chomczynski et al.DNA digestion was carried out after RNA extraction using DNase I. RNA quality was determined by examining A260/A280 with a NanoDrop One spectrophotometer (   RNA integrity was confirmed using 1.5% agarose gel electrophoresis. Qualified RNAs were finally quantified using Qubit3.0 with a Qubit RNA broad-range assay kit (Life Technologies, Q10210). 2 μg total RNAs were used for stranded RNA sequencing library preparation using a KCTM Stranded mRNA Library Prep Kit for Illumina (Catalog No. DR08402, Wuhan SeqHealth Co., Ltd., China) following the manufacturer's protocol. PCR products corresponding to 200–500 bps were enriched, quantified, and finally sequenced on a Novaseq 6000 sequencer (Illumina) with a PE150 model.