GSM8009124: HGG_38, Ampliseq; Homo sapiens; OTHER
Formalin-fixed paraffin-embedded tissue samples were obtained from the Pathology Service at the Asenjo Neurosurgery Institute. After neuropathologist confirmation of a tumor percentage greater than 70% in each area to be extracted, 3-6 sections of 10 microns were prepared from each paraffin block using the GeneJET FFPE DNA Purification Kit. No matched blood samples were available. The extracted DNA was quantified using fluorescence-based methods (Quant-iT™ PicoGreen™ dsDNA and/or Qubit™ dsDNA HS), resulting in an average concentration of 40 ng/µL. We For both AmpliSeq™ and TruSeq™ libraries, library generation efficiency was evaluated with different initial amounts of DNA to adjust the various stages of the process based on the specific sample and panel characteristics. Automated electrophoresis of the first libraries were performed for quality control and size estimation using the DNA 1000 kit on the Agilent 2100 Bioanalyzer system. All libraries were quantified by qPCR using the Illumina KAPA library quantification kit and were pooled at a concentration of 10 nM before sequencing. A Mid Output Kit v2.5 (150 cycles) was used on a NextSeq sequencer with 25% phi-X for all Ampliseq™ libraries, and a Micro Kit v2 (300 cycles) with 25% phi-X on a MiSeq sequencer was used for all TruSeq™ libraries. Amplicon sequencing