GSM8058813: SOA4-1_Zone_SZ_Condition_NWB_Rep3; Homo sapiens; RNA-Seq
Fresh cartilage tissue obtained from patients with OA and non-OA controls was placed in cold RNase-free PBS. The tissue was then gently transferred to pre-cooled optimum cutting temperature (OCT) compound, ensuring that the entire tissue was embedded. The tissue was immediately frozen in liquid nitrogen or dry ice and transferred to -80 °C. The OCT-embedded samples were cryosectioned at −20 °C at 14 μm thickness. The sections were then mounted on polyethylene-terephthalate-coated slides, fixed immediately with ethanol, and stained with 1% cresyl violet acetate dissolved in 75% ethanol solution. Afterward, targeted regions of sections were collected by laser capture microdissection (LCM), and transcriptome analyses of those collected samples were performed by Geo-seq. cDNA library was prepared using TruePrep DNA Library Prep Kit V2 for Illumina and sequenced on Illumina NovaSeq 6000 with PE150 mode.