GSM8240929: D7_YKL; Mus musculus; RNA-Seq
Seven days post-operation, callus tissue was meticulously harvested. A dissection approach was employed, deliberately avoiding the bone marrow to minimize hematopoietic cell carryover. The callus material was subjected to collagenase digestion (0.2% collagenase type I, 35°C/15 min/500 rpm) to obtain a single-cell suspension. Single cells were encapsulated into emulsion droplets using a Chromium Controller (10´ Genomics, Pleasanton, CA, USA). scRNA-seq libraries were constructed using Chromium Single-Cell 3' v3 Reagent Kit according to the manufacturer's protocol.