GSM6459734: dCTCF_C60_1; Homo sapiens; OTHER
Nuclei were isolated and nuclear run-on performed with biotinylated nucleotides. Biotinylated RNA transcripts were isolated using streptavidin coated magnetic beads. Constructed as detailed in Mahat et al. 2016. "Base-pair-resolution genome-wide mapping of active RNA Polymerases using precision nuclear run-on (PRO-seq). Nature Protocols. Aug 11(8):1455-76 doi:10.1038/nprot.2016.086