SGroup-ST1
Genomic DNA was extracted using TIANamp stool DNA kit (Tiangen) according to the manufactures instructions. The Metagenome sequencing was conducted by E-Gene Co., Ltd. The DNA was fragmented by sonication using a Bioruptor (Diagenode) to a mean size of approximately 300 bp. After purification, the fragmented DNA was subjected to blunt ending, dA addition to 3end and adaptor ligation. Finally, the libraries were generated by PCR amplification using adapter compatible barcode primers. The library quality was assessed using a Qubit 2.0 Fluorometer (Life Technologies, ThermoFisher Scientific, USA) and a 2100 Bioanalyzer system (Agilent Technologies, Inc., Santa Clara, CA, USA). Lastly, the library was sequenced on a Nova platform (Illumina), generating 150-bp paired-end reads.