Single cell RNA-sequencing of human melanoma
Single-cell gene expression (GEX) libraries were produced using the 10x Genomics Chromium Single Cell 5' Library and Gel Bead Kit v1 chemistry (10x Genomics; Cat 1000006), following the manufacturer's guidelines. After creating cDNA, quality control assessment was undertaken using the Qubit dsDNA High Sensitivity kit (Invitrogen; Cat Q32854) for concentration evaluation and the Agilent High Sensitivity BioA DNA kit (Agilent; Cat 5067-4626) for measuring size distribution. Samples were then sequenced on the NextSeq 500 sequencer using the 75 cycles flow-cell/kit (Illumina; Cat 20024906) with paired-end reads: 26 reads for the first read and 55 for the second.