GSM6940830: THP1, Control, Input; Homo sapiens; OTHER
Genomic DNA was extracted using the DNeasy Blood & Tissue Kit (Qiagen) and fragmented by sonication to a mean size of approximately 200-400 bp hMeDIP-seq libraries were prepared using the KAPA HyperPrep Kit (KAPA, KK8505) according to the manufacturer's protocol. The anti-5hmC antibody (Diagenode) was used to immunoprecipitate the adaptor-ligated DNA. This captured DNA was then purified with Zymo DNA Clean & Concentrator™-5 (Zymo Research) and subsequently amplified by 13-15 cycles of PCR using Illumina 8-bp dual index primers. Libraries were validated using Qubit.