16S analysis of Germ-free swiss mice that were previously colonized with microbiota from Swiss SPF mice maintened for 30 days in inulin diet and then fed with a conventional chow for 21 days - subject GM-I2
The DNA sample was added to the buffer solution and multiple DNA polymerases after fragment selection to fully repair the damaged DNA sample and add an A base to the 3'end at the same time. The adapter connection reaction system was configured to react in the PCR machine, and the sequencing adapter was ligated with Index to the end A base. The sample obtained was then amplified by PCR reaction and the PCR products were purified and recovered by magnetic beads.