GSM6914624: s3, scRNAseq; Homo sapiens; RNA-Seq
PI was added prior to sorting to exclude non-viable cells. Live CD45+ and CD45- populations were sorted and adjusted to 1 x 103/ml.  Samples with >85% cell viability were processed at the Genomics Birmingham Sequencing Facility (University of Birmingham, UK) for gene expression profiling using the 10X Genomics platform. Around 1.7 x 104 cells per sample were processed using the Chromium Controller (10X Genomics) for a recovery of 1 x 104 cells per sample, and library preparation was performed using the Chromium Single Cell 3′ Library & Gel Bead Kit v2 according to manufacturer's instructions. Library quantification and quality control was performed using TapeStation (Agilent). Samples were sequenced on an Illumina NextSeq 500 (150 bps, paired-end) at a sequencing depth of >50,000 raw reads/cell. Illumina NextSeq 500 (150 bps, paired-end) at a sequencing depth of >50,000 raw reads/cell.  