GSM7498858: RNA-Seq siLMO2_2; Homo sapiens; RNA-Seq
ChIP-Seq: DNA and protein were cross-linked by 1% formaldehyde. Chromatin was isolated and sonicated into 100-300 bp fragments. Then the DNA-protein complex was precipitated with control IgG or antibodies against LMO2 overnight at 4°C followed by protein A/G conjugated magnetic beads for 1 hr. Cross-links were reversed to extract DNA. RNA-Seq: HUVECs cells (3.3×106) transfected with LMO2 or negative control siRNA for 72 hr were harvested. RNA was extracted using the FastPure cell/tissue total RNA isolation kit V2 (Vazyme, cat. RC112-01). ChIP-Seq: 10 ng enriched DNA and input DNA were sent to Novogene for library preparation using the KAPA HTP library preparation kit and sequenced in Illumina NovaSeq 6000 to obtain 20 million read pairs per sample. RNA-Seq: RNA samples were submitted to Annoroad Gene Technology Corporation (Beijing) for library construction and sequencing. Briefly, following the RNA quality check, ribosomal RNA was removed using Epicentre Ribo-ZeroTM Gold  Kits. Then sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (#E7530L, NEB, USA) following the manufacturer's manual.