GSM7846598: immature scar, s1; Homo sapiens; RNA-Seq
Frozen sections of immature scars and keloids were stained with TAGLN. TAGLN-positive areas were collected from serial sections by manual dissection. The total RNA from the obtained tissue was extracted by ReliaPrep RNA Tissue Miniprep System (Promega, Madison, WI, USA). 10ng of total RNA was used for the construction of sequencing libraries. Libraries were constructed using the QIAseq UPX 3' Transcriptome Kit (QIAGEN, Hilden, Germany) following the manufacturer's protocols.