GSM7501988: 26-29 hours after egg lay, anterior part of the germband, rep2; Tribolium castaneum; ATAC-seq
Embryos were dechorionated by immersion in 1 % bleach for 30 s twice. Selected embryos were dissected into three parts (anterior, middle, and posterior). Embryo parts were homogenized using a plastic pestle in ice-cold lysis buffer. The nuclear pellet was collected by centrifugation. For tagmentation, 0.5 µl of Tn5 transposase from the Nextera DNA Library Prep Kit (Illumina) was used in a reaction volume of 15 µl. The reaction mix was incubated for 8 min at 37 °C.  Sample purification was performed using the MinElute PCR Purification Kit (QIAGEN).