Oxford nanopore long read sequences fastq of HVKP1
genomic DNA was extracted from overnight cultures grown at 37C in Mueller Hinton broth, using Fire Monkey High Molecular Weight DNA Extraction Kit (RevoluGen). Libraries were prepared using a ligation sequencing kit (SQK-LSK109) and native barcoding kit (EXP-NBD104) according to the manufacturers guidelines (Oxford Nanopore Technologies). Long-read sequencing was performed using an R9.4.1 flow cell (Oxford Nanopore Technologies) on a MinION device, running for 72 h. The reads were base called using a high accuracy base calling mode, and demultiplexed using Guppy (v6.2.1). Seqkit (v2.2.0) was used to determine number of basepairs, number of reads and the read N50