GSM6944362: bbs2_WT3; Danio rerio; RNA-Seq
Total RNA was purified using the standard Trizol (Thermofisher AM2238) protocol with Glycoblue (Thermofisher AM9515) used as a co-precipitant. Samples were Dnase treated (Turbo DNase, Thermofisher AM2238) and purified via lithium chloride precipitation. RNA concentration and purity was analyzed by Qubit Fluorometer (Invitrogen) and Agilent Bioanalyzer 2100. All samples had an RNA >= 8.8. 500ng of total RNA was used for quality control and the construction of sequencing libraries. Libraries were prepared from total RNA using Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus kit according to the manufacturer's protocol (Document #1000000124514 v02). Libraries were prepared by the Lerner Research Institute Genomics Core at the Cleveland Clinic.