GSM6432778: c30 cjPGCLCs, shallow WGBS, rep1; Callithrix jacchus; Bisulfite-Seq
Cells were collected and lysed in 50 mM Tris (pH 8.0), 10 mM EDTA, 0.5% SDS, 100µg/ml Proteinase K. Then crude DNA was used to build methylation sequencing library according to the protocol of Pico Methyl-Seq Library Prep Kit (Zymo, #D5455). Then libraries were sequenced on Illumina HiSeq 2000. Pico Methyl-Seq Library Prep Kit.