GSM6398802: ZNF506 H3K9me3 ChIP; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. Crosslinked chromatin from 2-4X107 cells fragmented on a Covaris to ~100-300bp was immunoprecipitated with GFP (Invitrogen A-6455), H3K9me3 (Abcam ab8898) antibodies or KAP1 (Abcam ab22553). library construction using the KAPA Hyper Prep Kit (Roche)