miRNA-Seq of Eriocheir Sinensis: Juvenile male crab
The 3' and 5' adaptors were ligated to the 3' and 5' ends of the small RNA, respectively. Then, first-strand cDNA was synthesized after hybridization with reverse transcription primers. The double-stranded cDNA library was generated through PCR enrichment. After purification and size selection, libraries with insertions between 18-40 bp were ready for sequencing via Illumina sequencing with SE50. The library was checked with a Qubit fluorometer and real-time PCR for quantification and a bioanalyzer for size distribution detection. The quantified libraries will be pooled and sequenced on Illumina platforms according to the effective library concentration and the amount of data needed.