GSM7877347: E11.5 FNP/MxP/MdP sonicated and SC184 immunoprecipated chromatin; Mus musculus; ChIP-Seq
Chromatin immunoprecipitation (ChIP) was conducted, essentially as previously described (Van Otterloo et al., 2022), with the exception that full facial prominences were used, rather than just isolated ectoderm. Further modifications included the use of an anti-TFAP2A antibody (5 µg of sc-184, Santa Cruz Biotechnology) and Dynabeads (ThermoFisher Scientific). Following precipitation and subsequent quality control analysis (ScreenTape analysis, Agilent), samples (i.e., sc-184 IP and input DNA) were sequenced on the Illumina HiSeq 2500 platform as 150-bp single-end reads. Library construction and sequencing was carried out by the Genomics and Microarray Core at the University of Colorado Anschutz Medical Campus.