GSM8284014: 21046X20_Islet_KD_replicate1; Mus musculus; RNA-Seq
Trizol Chloroform Intact poly(A) RNA was purified from total RNA samples (10-500 ng) using the NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490Stranded RNA sequencing libraries were prepared as described using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760L).  Purified libraries were qualified on an Agilent Technologies 4150 TapeStation using a D1000 ScreenTape assay (cat# 5067-5582 and 5067-5583).  The molarity of adapter-modified molecules was defined by quantitative PCR using the Kapa Biosystems Kapa Library Quant Kit (cat#KK4824).  Individual libraries were normalized to 5 nM in preparation for Illumina sequence analysis.