GSM6832932: inoculum 0h Giardia DMEM/F-12+FBS cond biol rep 2; Giardia intestinalis assemblage A; RNA-Seq
Total RNA extractions were performed using Trizol according to the manufactures instructions. RNA was then treated with DNaseI and extracted again by phenol chloroform extraction. Sequencing libraries were prepared from 500ng total RNA using the TruSeq stranded mRNA library preparation kit (cat# 20020595, Illumina Inc.) including polyA selection. Unique dual indexes (cat# 20022371, Illumina Inc.) were used. The library preparation was performed according to the manufacturers' protocol.