GSM6944220: CutnTag: Embryonic day 10.5 wildtype mouse r4 motor neurons with NR2F1 antibody rep2; Mus musculus; OTHER
Single cell suspensions of microdissected embryonic hindbrain tissue was performed using the Papain Dissociation System (Worthington). Isl1mn-GFPon cells were isolated using FACS, with the gates opened to GFP-negative cells at the conclusion of the sort to a total cell number of approximately 10,000-12,000 “Single Cell CUT and Tag on 10X genomics platform” protocol from www.protocol.io and 10x Genomics Chromium Next GEM Single Cell ATAC Kit