GSM8239725: IgG C&R Ctl; Mus musculus; OTHER
mechanical trituration - P0 dissected murine retina Cut&run-seq was performed using the Cutana cut&run kit (14-0500; Epicypher), following the manufacturer's instructions. Briefly, P0 retinas were dissected. Cells were dissociated with mechanical trituration in order to retain the glycoproteins necessary for adhering to the concavanalin beads. 500 000 cells were used in each reaction. Cut&run seq was performed using antibodies against Chd4 (Abcam ab72418) or Mbd3 (Fortis A302-529A). Non-specific IgG was used as a negative control. Libraries were prepared using the NEBNext® UltraTM II Library Prep Kit (New England Biolabs). Paired-end 150 sequencing was performed using the NextSeq 500 platform (Illumina) to a read-depth of ~20-35 million reads per sample.