WGS of Staphylococcus haemolyticus: preterm infant stool
taphylococcus haemolyticus strains were isolated from 46 preterm infant stool samples as part of the Baby-Associated MicroBiota of the Intestine (BAMBI) study. A total of 142 isolates were cultured, selectively identified as presumptive coagulase-negative staphylococcus (CoNS), and subjected to whole-genome sequencing using Illumina and Oxford Nanopore Technologies. Genomic DNA extraction involved specific protocols, including the addition of lysozyme and lysostaphin, followed by library preparation, normalization, and sequencing on Illumina Nextseq500 and MinION platforms.