GSM6569798: Hyg-AGX1_F383A-NahK_unfed K562_3b; Homo sapiens; RNA-Seq
RNA was extracted from fed and unfed cells by using RNeasy Mini kit (Qiagen, Crawley, UK), DNase I (Thermo Fisher) and QIAshredder (Qiagen) according to the manufacturer's instructions. mRNA capture and library preparation were performed using the KAPA mRNA HyperPrep Kit according to the manufacter's instructions