GSM7846751: Ncs2elp6hel2_1_mono; Saccharomyces cerevisiae; OTHER
Cell lysates were digested with Rnase I, layared on top of a sucrose gradient and run on an ultracentrifuge. Gradients were then fracrionated. RNA from sucrose fractions containing monosomes or disomes was extracted with acidic phenol. Libraries were prepared according to (McGlincy & Ingolia, 2017)