GSM8395820: HSSYII_MS67_H3K4me3; Homo sapiens; OTHER
CUT&Tag was performed with a commercial kit (EpiCypher CUTANA pAG-Tn5) based on the vendor's specification. In brief, 100,000 of human cancer cells were used, and all sample groups were added with 5,000 of NIH-3T3 cells as the spike-in normalization control, followed by CUT&Tag using antibodies of either WDR5, H3K4me2, H3K4me3, SS18-SSX or BAF155, the library preparation and deep sequencing (using an Illumina NextSeq 500 machine). DNA purification was carried out with the PCR cleanup kit (NEB, Monarch PCR & DNA Cleanup Kit, #T1030) followed by the PCR-based amplification using i5/i7 primers.