GSM8007905: SK-N-BE(2) shControl_rep1_Input; Homo sapiens; RIP-Seq
Total cellular RNA extracted from cells were fragmented by RNA fragmentation reagents (Thermo, AM8740). Fragmented RNA was incubated with anti-m6A antibody at 4°C for 6 h in 500 μl of NT2 buffer (200 mM NaCl, 50 mM HEPES pH 7.6, 2 mM EDTA, 0.05% NP-40, 0.5 mM DTT, 200 U ml–1 RNase inhibitor). RNA libraries were prepared for sequencing using standard Illumina protocols.