GSM6943644: CON [GSM2011000]; Homo sapiens; RNA-Seq
Cells were harvested and total RNA was extracted by using RNAmini kit ( Qiagen, Germany). Enrichment of mRNA, fragmentation, reverse transcription, library construction, Illumina Novaseq 6000 and data analysis were performed by Genergy Biotechnology Co. Ltd. (Shanghai, China). Total RNA was isolated from each thymic sample using the RNAmini kit (Qiagen, Germany). RNA quality was examined by gel electrophoresis and with Qubit (Thermo, Waltham, MA, USA). For RNA sequencing, RNA samples from different groups were separated into three independent pools, each comprised of two or three distinct samples, at equal amounts. Strand-specific libraries were constructed using the TruSeq RNA sample preparation kit (Illumina, San Diego, CA, USA), and sequencing was carried out using the Illumina Novaseq 6000 instrument by the commercial service of Genergy Biotechnology Co. Ltd. (Shanghai, China).