GSM8354203: BlaER_96h; Homo sapiens; Bisulfite-Seq
DNA was isolated using Bllood & Cell Culture DNA Mini Kit (QIAGEN). Treatment to produce single-strand DNA (ssDNA). During the bisulfite conversion process, unmethylated cytosines were converted into uracils, while methylated cytosines remained unchanged. Following this, methylation sequencing adapters were ligated, and double-strand DNA synthesis was carried out. The library was considered ready after size selection and PCR amplification. Illumina sequencing was performed with a PE150 (read length).