GSM8395425: scRNAseq, Py8119 tumor, Rk20449  treated, rep3; Mus musculus; RNA-Seq
Py8119 tumors were minced with scalpel blades and dissociated in 0.1 mg/ml DNAse 1 and 1.5 mg/ml collagenase type 4 for 1 hour at 37°C with an additional 0.1 mg/ml of DNAse 1 added for 30 minutes. Dissociated tumors were then passed through a 100µM cell strainer followed by red cell lysis. Aliquots of 1x106 cells were stored at -80°C in 50% FCS, 10% DMSO in F12K media. Frozen aliquots were thawed for use in single cell experiments. For the Vehicle and RK20449 treated tumors, the 10x Chromium 3′ v2 workflow was used according to the manufacturer's directions with approximately 9000 cells captured per sample. All other tumors were processed using the 10x Chromium 3′ v3 workflow and approximately 6000 cells captured per sample.