GSM6697859: K36M_DMOG_d4_WGBS_rep1; Mus musculus; Bisulfite-Seq
DNA for WGBS was extracted using a Qiagen Blood & Tissue DNeasy kit according to the manufacturer's recommendations. WGBS libraries were produced with the TrueMethyl oxBS-Seq Module (Tecan) and Accel-NGS Methyl-Seq DNA Library Kit (Swift) according to the manufacturer's instructions with the omission of the oxBS steps in the TrueMethyl protocol. Briefly, 300ng of gDNA was sheared to average fragment size of 200 bp using a Covaris S2 sonicator for 1 minute 30 seconds with the following settings: duty cycle 10%, intensity 5, cycles per burst 200. Resulting sheared gDNA was concentrated using Agencourt AMPure XP beads (Beckman Coulter) and then subjected to bisulfite conversion and desulfunation as outlined in the TrueMethyl oxBS-Seq Module. This bisulfite converted gDNA was then used as input for the Accel-NGS Methyl-Seq DNA Library Kit and yielded sequencable libraries.