DNA metabarcoding (16S rRNA) of Trieste C1 LTER surface seawater
The V4-V5 region of 16S rRNA gene was amplified using 515-Y (5-GTGYCAGCMGCCGCGGTAA-3) and 926R (5-CCGYCAATTYMTTTRAGTTT-3) primers (Parada et al. 2016). Libraries were prepared following the 16S Metagenomic Sequencing Library Preparation protocol and run on an Illumina MiSeq System