GSM7498071: sgControl_3; Homo sapiens; RNA-Seq
Total RNA was isolated using a Qiagen RNeasy extraction kit. An on-column DNA digestion was performed to remove any genomic DNA contamination. For library preparation, 1 µg of RNA was used for each sample, rRNA-depletion using the NEBNext rRNA Depletion Kit v2 (#E7400, New England Biolabs) was performed, and library construction was completed using NEBNext Ultra RNA Library Prep Kit for Illumina (#E7760L, New England Biolabs) as per the manufacturer's instructions.