GSM7877413: HEK293T WT 2 tRNA library_replicate 3; Homo sapiens; ncRNA-Seq
Total RNA was extracted from cells using TRIzol reagent following the manufacturer's protocol. Transfer RNA was isolated from total RNA by gel extraction. RNA-seq libraries were prepared using the NEBNext® Small RNA Library Prep Set for Illumina® (NEB #E7330S) following the manufacturer's protocols.