Viral metagenome of pig: SW1
About 140 l of the supernatant from each sample pooled was pipetted and filtered through a 0.25 m filter (Millipore, Germany) to remove eukaryotic cell and bacterium-sized particles. The ten samples were pooled using equal volumes. As a result, each pool comprised ten specimens from the same species, sampling sites, and the time of collection and then stored at -20 C until further procedure. The enriched viral nucleic acid preparations from the respective pools, non-specific DNA amplification, were performed by random RT-PCR, using a primer with randomized octomer at the 3' ends. The amplified viral nucleic acid libraries were sequenced as 150 bp paired ends on the Illumina MiSeq platform.