Triticum turgidum cv. Kronos. INTACT-ATAC-seq data from frozen root tip samples.
2,000 purified nuclei were used in each transposase integration reaction using Nextera reagents. DNA fragments were purified using a column-based kit (DNA Clean & Concentrator-5). 10 ml of each sample were amplified using High Fidelity PCR Mix. The amplified libraries were then purified using a column-based kit (DNA Clean & Concentrator-5), and quantified using qbit (Invitrogen). For ATAC enrichment check by qPCR, the amplified libraries were diluted 10 times in water, of which 5 l were used in qPCR reaction. Quantitative PCR was performed using SYBR Green and a 7500 Fast Real-Time PCR system.