GSM6735148: MCF7, shPURA2, rep 1; Homo sapiens; RNA-Seq
Cells were directly subjected to RNA extraction using TRIzol reagent (Thermo Fisher Scientific). 1.0 ug of total RNA was used for the construction of sequencing libraries. The libraries was sequenced on DNBseq(PE150) platform. DNBSEQ Eukaryotic Strand-specific mRNA library strategy was applied to establish libraries.