GSM7846495: 1 Pediatric food anaphylaxis_basal; Homo sapiens; miRNA-Seq
Total RNA was isolated from 200 μl of serum using the miRNeasy Serum/Plasma Kit (Qiagen) according to manufacturer's instructions Libraries were performed with the QIAseq miRNA Library Kit (Qiagen). RNA was retrotranscribed to copy DNA (cDNA), amplified by PCR (22 cycles) and purified from samples. Libraries were carried out by a gel-free system through the addition of Unique Molecular Identifiers (UMIs) and were specific for small RNA species ranging from 15-40 nucleotides.