GSM8008785: PDAC_SMAD2_ChIP; Mus musculus; ChIP-Seq
Cells were cross-linked with 1% paraformaldehyde and then quenched with 0.125 mol/L glycine. Subsequently, cells were lysed on ice for 30 minutes using lysis buffer. NEBNext Ultra DNA Library Preparation kit was used following the manufacturer's recommendations (New England Biolabs, Ipswich, MA, USA). Briefly, the ChIP DNA was end repaired and adapters were ligated after adenylation of the 3'ends. Adapter-ligated DNA was size selected, followed by clean up, and limited cycle PCR enrichment. The ChIP library was validated using Agilent TapeStation and quantified using Qubit 2.0 Fluorometer as well as real time PCR (Applied Biosystems, Carlsbad, CA, USA).