GSM6395069: H9 DGCR8 heterozygote HET2 clone replicate 2 (miRNA-seq); Homo sapiens; miRNA-Seq
RNA  was extracted using mirVana microRNA isolation kit (ThermoFisher). Small RNA libraries were generated using NEXTFlex Small RNA Library Prep Kit v3 (Cat #NOVA-5132–06). The manufacturer's instructions where followed through PCR amplification. The optional stop point was used post the 5' 4N Adapter Ligation prior the Reverse Trascription reaction. In regards to PCR amplification the 200 ng samples received 20 cycles. Following PCR amplification, the libraries were run in a 6% TBE-PAGE Gel for 50 min at 140 V. The 140-170 bp band were excised from the gel and purified by beads. Once purified, libraries were multiplexed and concentration and size check of final pool were measured by Qubit dsDNA HS Assay (Life Technologies) and Bioanalyzer High Sensitivity DNA Assay (Agilent Technologies), repectively