RAD-Seq: FS-7460 (run1) cross I05 (INRA09-FS0813, Mat1-1) and I07 (INRA09-FS0732, Mat1-2)
Each offspring individual was grown over 7 days in a 250mL Erlenmeyer flask containing 100mL of YPD (Yeast extract Peptone Dextrose) with constant agitation at 160rpm. At the end of the seven-day growth period, a maximum of culture medium was separated from the spores, which were then transferred into 50mL Falcon tubes to be lyophilized for 24 to 30 hours. After lyophilisation, the completely dry samples were ground in liquid nitrogen with a mortar and pestle. DNA was extracted from each sample following a phenol/chloroform-based protocol.