GSM6598722: ΔdgcO anaerobic 3; Pectobacterium carotovorum subsp. carotovorum; RNA-Seq
Total RNA was extracted from the resuspended pellets using the Monarch® Total RNA Miniprep Kit #T2010S (NEB, Ipswich, MA, USA) following the manufacturer's instructions. Extracted RNA was sent on dry ice to Seqcenter, LLC (Pittsburgh, PA, USA) where all following steps, incuding data processing, were completed. Samples were subjected to ribosomal RNA depletion using the Ribo-Zero™ Plus rRNA Removal Kit and complementary DNA (cDNA) libraries were then prepared with the Illumina Stranded RNA library preparation kit.