GSM6734574: Rich Media (YPD) and Minimal Media (MMD) Single Cell Genotype Barcodes; Saccharomyces cerevisiae; RNA-Seq
5mL of media (YPD: 4.25e7 cells/mL, MMD: 1.07e7 cells/mL) spun down, and cells resuspended in 1mL saturated ammonium sulfate (RNAlater). Cells washed once with 1mL RNAlater, and then stored overnight in -20C in 500 uL RNAlater. Cells are processed into cDNA libraries using the 10X 3' gene expression kit v3. Sequencing libraries are then further prepared and indexed from cDNA with the 10x 3' gene expression kit v3. Genotype barcodes are extracted by PCR from cDNA libraries and indexed separately.