Platelets favor the outgrowth of established metastases
Platelets favor the outgrowth of established metastases
Samples were 10m thin slides of paraffine embedded lungs stocked at room temperature.  NucleoSpin totalRNA FFPE XS Micro kit (Cat.740969.50, Macherey-Nagel) was used according to manufacturer's instruction and RNA was eluted in 30μg/mL DNaseI under gentle mixing at 37°C for 10L final volume. RNA concentration was assessed after extraction at the NanoPhotometer® N60 (Implemen). RNA integrity (>50% DV200) was assessed by Bioanalyzer (total RNA Pico Kit and RNA 6000 Nano Kit, 2100 Instrument, Agilent Technologies, Paolo Alto, CA, USA). Sequencing libraries were prepared using “NEBNext Ultra II Directional RNA Library Prep Kit for Illumina“ combined with “NEBNext rRNA Depletion Kit v2” for ribosomal RNA depletion (New England Biolabs, Ipswich, MA).