GSM6432800: CUT&RUN sequencing of scrambled control A375 cells (RNA pol II antibody) [RNAPII_shSCR]; Homo sapiens; OTHER
After purification of the extracted DNA with DNA Purification Buffers and Spin Columns kit (Cell Signaling Technologies). Size selection was performed using SPRIselect beads (Beckman Coulter) and fragment sizes were determined with an Agilent 2100 Bioanalyzer using the High Sensitivity DNA Kit (Agilent). The double stranded DNA content of the libraries were quantified with Qubit 3.0 using the Qubit dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific) followed by equimolar pooling of the indexed libraries. Sequencing libraries were generated using NEBNext ULTRA II DNA library Prep Kit for Illumina (New England Biolabs) with indexed NEBNext Multiplex Oligos for Illumina (New England Biolabs)