GSM7501611: HEK293, WT, MLX ChIP, Replicate 1; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 5 min. After quenching and cell lysis chromatin was fragmented using QSonica Q800R sonicator. Total amount of 5 ug of human chromatin and 100 ng of Drosophila chromatin was used for IP. ChIP-seq libraries were prepared using NEBNext Ultra II DNA Library Prep Kit with the adapter dilution 1:25 and 5 uL pre-mixed forward/reverse primer solution. The starting mass of DNA for the library preparation was 5 ng. Libraries were amplified in 11 PCR cycles. Double size selection of DNA fragments of a size 200-600 bp was performed using magnetic AMPure XP beads (Beckman Coulter) and validated using Bioanalyzer High Sensitivity Chip (Agilent).