GSM8283750: Human skin fibroblasts, si-HTRA1-1; Homo sapiens; RNA-Seq
For gene silencing, human pathological scar fibroblasts were transfected with 100 nM siRNA using Lipofectamine RNAiMAX reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's experimental protocol. RNA was harvested using Rneasy mini plus kit (Qiagen). 1.3 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.