GSM6460075: HAP, wild-type, IFNg, Rep1; Homo sapiens; RNA-Seq
HAP1-Cas9 cells were first established by lentiviral introduction of Cas9 and Pbrm1 were knockouted in bulk using sgRNA. Infected cells were selected with both blasticidin and puromycin to ensure cells expression both Cas9 and sgRNA. Pbrm1-intact and Pbrm1-deficient cells were treated with or without 50 ng/mL. IFNg for 5 days. RNA was harvested using RNeasy Mini Plus kit (Qiagen) and resultant RNA were Dnase-treated before library preparation. 200 ng RNA were used for subsequent library preparation RNA-seq libraries were prepared from poly-A-enriched mRNA using a KAPA mRNA HyperPrep Kit (Roche) and sequenced on an Illumina Novaseq.