Susceptible bulk from PxG F2 population
Libraries were prepared using the Illumina TruSeq DNA Nano Library Preparation Kit.  Completed libraries were QC'd using Qubit dsDNA HS, Caliper LabChipGX HS DNA and Kapa Biosystems Illumina Library Quantification qPCR assays.  After quantitation, libraries were pooled in roughly equimolar amounts and this pool was loaded on one (1) lane of an Illumina HiSeq 2500 High Output flow cell (v2).  Sequencing was carried out using HiSeq SBS reagents in a 2x125bp paired end format (PE125).