WGS of primary colon cancer from P8823: Sample 8823_22354B.D01_L015242_WGS_1159
DNA was extracted from frozen tissue using PureLink Genomic DNA Mini Kit. WGS libraries were prepared using NEBNext Ultra DNA Library Prep kit. Genomic DNA was fragmented by acoustic shearing with a Covaris S220 instrument. Fragmented DNA was cleaned up and end repaired. Adapters were ligated after adenylation of the 3'ends followed by enrichment by limited cycle PCR.