GSM8098235: Setd8KD_ATAC_2; Mus musculus; ATAC-seq
mRNAs were collected using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen), and the purified mRNAs were subjected to cDNA library construction using a NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturer's protocols. ATAC-seq was performed as previously described by Buenrostro, et al. (PMID:2559105). After transpisition reaction with transposase enzyme in Illumina Nextra DNA Preparetion Kit (#FC-121-1030). For H4K20me1 analysis, CUT&RUN (PMID: 28079019) was employed. Sequencing libraries for CUT&RUN were made using the NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina and NEBNext Multiplex Oligos for Illumina (New England Biolabs) Llibraries were prepared for sequencing following standard Illumina protocols RNA-seq, ATAC-seq, CUT&RUN