GSM7719935: VGAT+ sorted sample 3; Mus musculus; RNA-Seq
Dorsal hippocampi were microdissected at P8 in Hibernate A buffer without Ca2+. Tissue chunks were dissociated in papain (2 mg/mL in Hibernate A) at 37 C for 60 minutes and inverted 4-5 times every 5 minutes. DNAseI (1 mg/mL) was added, and tissue was triturated through pipettes of decreasing diameter and supernatants kept on ice. Supernatants were filtered through 70 um filter, centrifuged at 500 rcf for 10 min at 4 C. The pellet was resuspended in PBS with 3% FBS and DAPI for FACS. Fluorescent cells were collected using a 100 um nozzle. Collected cells were centrifuged at 4 C, 500 rcf for 10 minutes, resuspended in TRIzol LS, and frozen on dry ice. Libraries were prepared using the Illumina Truseq protocol.