GSM7403854: RNA-Seq - Replicate2 of optimal donor EX28-1; Homo sapiens; RNA-Seq
By applying RNA  and ATAC sequencing, we want to investigate the transcriptomics and epigenetic factors that contributing for difference between optimal and suboptimcal  in NK cells scATAC-Seq: Single‐nucleus libraries were generated using the 10X Genomics Chromium Next GEM ATAC Capture and Library (V1.1), following the manufacturer's protocol. Nine samples were pooled to give a final concentration of 10 nM. Pooled samples underwent further qPCR prior to sequencing with the NovaSeq 6000 sequencer using the S1, 100 cycles flow cell and sequenced with read 1 for 50 cycles, i7 8 cycles, i5 index 16 cycles, and read 2 for 49 cycles. ATAC-Seq: Libraries were sequenced  2 x 100 bp on an Illumina NovaSeq6000 to obtain at least 50 million high quality mapping reads per sample. RNA-Seq: Libraries were prepared using Illumina next generation RNA sequencing .