GSM8005845: SJ donor 1 and SJ donor 3 Biopsy (SJ01_SJ03_Biopsy) - CSP; Homo sapiens; RNA-Seq
Salivary gland biopsy were collected from all donors and stored in cryostor. Biopsy was disaggregated using a protocol published by the AMP consortia and CD45+ cells from all thawed disaggregated ST samples were sorted via flow cytometric activated cell sorting. The 10x Genomics Chromium instrument was used to partition each single cell with barcoded beads. GEM generation and barcoding, cDNA amplification, cell surface protein and library construction was performed according to the 10X Genomics Chromium User Guide. Sequencing was performed using the Illumina NovaSeq 6000.