GSM6735271: HM_DFX50; Mus musculus; RNA-Seq
The total RNA of cells was isolated using the High Pure RNA Isolation Kit (Roche Life Science). The Genetic Analysis company (Rhelixa, Tokyo, Japan) performed whole-transcriptome sequencing (Bulk RNA-seq) utilizing the NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Ipswich, Massachusetts) and NEBNext® UltraTMII Directional RNA Library Prep Kit (New England Biolabs, Ipswich, Massachusetts). After ribosomal RNA (rRNA) was depleted, conventional Illumina techniques were performed to generate sequencing libraries, which were then processed on an Illumina NovaSeq 6000 sequencing machine (150-bp paired-end reads, two samples per lane; Illumina, San Diego, California).