GSM8240930: Pcif1-KO_PCIF1-WT-rescue_anabolicSLAM; Homo sapiens; RNA-Seq
48hr later, cells were harvested by scraping and nuclei was enriched using Nuclei EZ (Sigma NUC-101). Nuclei were homogenized in Trizol-LS and nuclear RNA was isolated using Phase-Lock (HEAVY) tubes according to manufacturer's protocol. Residual genomic DNA was removed using RQ1 RNase-free DNAse (Promega M6101) before RNA was purified with RNA concentrator (Zymo R1015). RNA-seq library was prepared using QuantSeq 3' mRNA-Seq Library Prep Kit-REV (Lexogen 016). 3' mRNA-Seq