the 35th Multicenter Airway Research Collaboration (MARC-35).
We prepared RNA for small RNA-seq using the PerkinElmer NEXTFLEX® small RNA-seq v3 kit with Unique Dual Indexes (PerkinElmer, Waltham, US) and sequenced on an Illumina NovaSeq6000 sequencer using an S2 50bp PE Flowcell (Illumina, San Diego, CA). All small RNA-seq samples had sufficient sequence depth (mean, 26,736,490 pair-end reads/sample; min, 2,128,932 pair-end reads/sample; max, 93,053,738 pair-end reads/sample) to obtain a high degree of sequence coverage. We estimated microRNA (miRNA) detection and abundance using sMETASeq. Fastq files underwent quality control in cutadapt and collapse into unique reads.