GSM6598057: Nuclei from P1 animals (Sample 2A); Mus musculus; RNA-Seq
Portions of the ventral brainstem were dissected and tissue was manually dissociated with a dounce homogenizer and nuclei were isolated using differential centrifugation. Enrichment for cholinergic nuclei was achieved by collecting GFP+ nuclei via FACS. Individual nuclei were captured through the 10x Genomics Platform. Libraries were generated following manufacturer's directions (Single Cell 3' v3 for Samples 1A-4, Single Cell 3' v2 for Samples 5-8).