GSM7421451: Replicate 2, dura mater, wild-type, 9-month-old, male; Mus musculus; RNA-Seq
Dorsal dura mater from four different PBS-perfused mice were pooled as one sample. Freshly collected and cut tissues were dissociated with enzymatic digestion (Collagenase VIII and DNase I) and mechanical dissociation with pippeting. Cells were washed with Ca2+ and Mg2+ free HBSS and strained with 70-µm cell strainer. DAPI- viable singlets were sorted with FACS into 1.5 ml tube and kept in PBS with 0.04% BSA. The Chromium Single Cell 3'RNAseq run and library preparation were done using the Chromium™ Single Cell 3' Reagent version 3 chemistry according to the manufacter's instructions.