Alex_Yellow_Male
We extracted total cellular RNA from a single adult male snail by placing whole-body tissue in Qiagen RNAlater following manufacturer protocols, and flash-froze the sample in liquid nitrogen and stored at -80C until RNA extraction. RNA was extracted with Trizol and cleaned with Zymo RNA Clean and Concentrate following manufacturer protocols. We produced cDNA libraries for sequencing with the TruSeq RNA Sample Prep v2. All transcriptomes were sequenced with Illumina HiSeq 2500 2x100 bp sequencing.