Shotgun sequencing of Dungeness crab with HiSeqX10
Starting with 2ug of genomic DNA the sample was sheared to an average size of 250bp using the Covaris LE220 focused ultrasonicator.  The resulting sheared DNA was cleaned using Agencourt AMPure XP beads.  End-repair, A-tailing, and ligation were performed as directed by the Kapa Hyper Prep Kit.