GSM8239735: PKM2ΔMAC, MCD8w, scRNA-seq; Mus musculus; RNA-Seq
Nonparenchymal cells were isolated by liver perfusion and density gradient centrifugation RNA from the barcoded cells was subsequently reverse-transcribed and sequencing libraries constructed with reagents from a Chromium Single Cell 3' v2(v2 or v3, depends on project) reagent kit (10X Genomics) according to the manufacturer's instructions. Sequencing was performed with Illumina (HiSeq 2000 or NovaSeq , depends on project) according to the manufacturer's instructions (Illumina)