GSM8186650: H4K16ac-WT-R848; Mus musculus; OTHER
CUT&Tag was performed using the Hyperactive Universal CUT&Tag Assay Kit for Illumina (Vazyme) according to the product instructions. Briefly, WT and KAT8-deficient BMDMs were stimulated with R848 for 2h, and then harvested after PBS wash. Cells (1 × 105) were bound with concanavalin A-coated magnetic beads and then incubated with antibodies against H4K16ac (CST) at 4 °C overnight. On the next day, samples were incubated with a second antibody and then with Hyperactive pA/G-Transposon for 1 h. DNA was treated with TTBL at 37 °C for 1 h and extracted. The librarys were constructed by purified DNA, and amplified using Hyperactive Universal CUT&Tag Assay Kit for Illumina (Vazyme) according to the product instructions.