small RNA-seq of spermatozoa from Testis cancer patients 1
Sperm from men with normal semen parameters and without any pathological context were used as controls. In total, the study population consisted of 16 patients with TGCT and 9 men with no TGC. Preparation of the 25 libraries and sequencing were performed by the Qiagen service provider, starting from 10 ng total RNA and using the QIAseq miRNA Library Kit.Deep sequencing was performed on Illumina HISeq2000, targeting 20 M single-reads (75 bp) per sample.