RNA sequencing of Tympanuchus phasianellus columbianus (juvenile male) RNA from heart tissue.
Total RNA extraction was done using the QIAGEN RNeasy Plus Kit following manufacturer protocols. Total RNA was quantified using Qubit RNA Assay and TapeStation 4200. Prior to library prep, we performed DNase treatment followed by AMPure bead clean up and QIAGEN FastSelect HMR rRNA depletion. Library preparation was done with the NEBNext Ultra II RNA Library Prep Kit following manufacturer protocols. Then these libraries were run on the NovaSeq6000 platform in 2 x 150 bp configuration.