GSM8097052: Replicate 1, scRNA-seq; Mus musculus; RNA-Seq
Femur, tibia, and ilium bones were harvested, cut open and crushed. Bone marrow cells were then passed through a 70-µm cell strainer and resuspended in FACS buffer. GEM RT Incubation, Post GEM-RT Cleanup, cDNA amplification, and Gene Expression Library Construction were performed as described in the Single Cell 3' Reagent Kits User Guide v3.1 RevD (10X Genomics). Prior to sequencing, the three single-cell libraries were quantified using the KAPA Library Quantification Kit for Illumina Platforms (Roche, 501965234) and pooled in an equimolar ratio.