GSM7847111: Immunoprecipitated RNA, replication 2; Homo sapiens; RIP-Seq
T-47D cells transduced with lentiviral inducible A3B were treated with 1 µg/mL doxycycline for 48 hours in petri dishes, followed by aspiration of culture medium and expose to 254 nm UV as previously described in a Stratalinker instrument. The cells were scraped, frozen, and submitted EclipseBio for further extraction. eCLIP-seq experiment and analysis were performs with two biological repeats, with anti-flag M2 monoclonal antibody (Sigma). Library was constructed using ENCODE standard eCLIP protocol.