RNA-Seq of klebsiella: children
And quality control software used to reduce data noise fastp (https://github.com/opengene/fastp), get effective data (clean) data. The retained reads were compared with the reference genome using Bowtie2 (version 2.2.8), and the genes that could be aligned were considered known genes. Then we analyzed the length and secondary structure of genes that did not match with reference genomes by RNAfold software . Genes with length of 50-300bp and stable secondary structure were listed as candidate sRNAs.