The complete genome sequence of Kineothrix sp. MB12-C1, isolated from feces of the black soldier fly larvae
For MinIONTM sequencer, sheared DNA fragments were straightly end-repaired, 3 adenylated, and then ligated to adaptors pre-loaded with motor proteins. The product was purified using Agencourt AMPure XP Beads (Beckman, A63881). Finally, fragments larger than 1 Kb were screened for single-molecule nanopore DNA sequencing using Multiplex Ligation Sequencing Kit (SQK-MLK111.96-XL) on MinION Flow Cell (ONT, R9.4.1).