GSM7720903: DRIP_seq_shTOP1_mDOX_Rnase_H_OE; Homo sapiens; OTHER
cells were crosslinked with 1% formaldehyde  and quenched with glycine. Fixed cells were washed in lysis buffer. Cells were sonicated using bioruptor sonicator to produce chromatin fragments of ~200-300 bp. The chromatin was immunoprecipitated by incubation with 5 μl of anti-TOP1cc antibody (MABE1048). Immune complexes were captured with protein G Dynabeads. DNA was precipitated by phenol/chloroform/isoamylalcohol extraction. DRIP-seq was done according to the published protocol (Sanz and Chédin, 2019). library was prepaired using kappa hyperprep kit