Genotyping by sequencing of Quercus robur: WWO_L4C
Samples of ~200 ng genomic DNA were normalised in 10 ?L of TE buffer and sent to the Genomic Analysis Platform, Institute of Integrative and Systems Biology, Universit Laval (Quebec, Canada). DNA was digested with PstI, NsiI, and MspI (C?CGG) according to the 3D-GBS protocol for library preparation. Sub-libraries were barcoded and pooled to equimolarity, then sequenced on an Illumina NovaSeq 6000 system with paired-end mode of 150 bp at the Gnome Qubec Innovation Centre (Montreal, Canada). Raw reads were demultiplexed with Sabre 1.0 and trimmed with Cutadapt 1.18 to remove the adaptors and sequences shorter than 50 bp.