Illumina NovaSeq 6000 paired end sequencing; Whole transcriptome analysis of pmel-1 CD8+ T cells treated with rat IgG, 3H3, and 3H3 plus TKS-1antibodies
Whole transcriptome analysis of pmel-1 CD8+ T cells treated with rat IgG, 3H3, and 3H3 plus TKS-1antibodies
B6 mice were intravenously injected with 5 × 10^5 pme-1 Thy1.1+CD8+ T cells. Subsequently, they were immunized with mgp100 peptide emulsified in IFA. At day 0 and 2, the mice were intraperitoneally administered with 100 μg of rat IgG, 3H3, or 3H3 plus TKS-1. After five days from the cell transfer, inguinal lymph nodes (LNs) were collected from each group of mice. Thy1.1+ cells were then isolated from the collected LNs using Thy1.1-microbeads (Miltenyi Biotec). RNA was extracted from the pmel-1 Thy1.1+CD8+ T cells using Trizol reagent (Invitrogen). Library was constructed using TruSeq Stranded mRNA Sample Prep Kit according to manufacturers protocol.