DEAD-box ATPase Dbp2 mediates mRNA release after 3'-end formation - II
DEAD-box ATPase Dbp2 mediates mRNA release after 3'-end formation - II
Cells were grown in EMMG, then shifted to YES for 9h. S. cerevisiae spike-in cells were added at at 1:5 OD600 ratio immediately before crosslinking with 1% formaldehyde for 20 min at room temperature. ChIP experiments were performed as described in Wittmann et al., Nat Commun 8, 14861 (2017). RNAPII was immunoprecipitated with antibody against Rpb1 (8WG16, Millipore) coupled to 20 µl of protein-G dynabeads (Life Technologies).  Libraries were prepared with the NEBNext Fast DNA Library Prep Set for Ion Torrent Kit (NEB).