GSM8396160: Pdgfbret/ret, replicate 1; Mus musculus; OTHER
Fresh frozen tissue preparation was done according to the protocol "Tissue preparation guide" (CG000240; 10x GENOMICS). All steps were performed using the 10x Visium Spatial Gene Expression Slide & Reagent kit. Methanol fixation and immunofluorescence staining were done according to protocol CG000312. The optimal tissue optimization time (4 min) was determined in a time course experiment according to protocol CG000238RevE. cDNA synthesis and library generation were done according to protocol CG000239RevD. cDNA synthesis was carried out according to the results obtained from qPCR testing with a KAPA FAST SYBR qPCR system (KAPA Biosystems). The amplified cDNA was processed according to protocol, using the SPRIselect Reagent kit (Beckman Coulter).