Etroplus suratensis, Iso-Seq data, FLNC reads, adult muscle, Pacbio sequel II
The total RNA was isolated by using Trizol (DSS Takara, CA, USA) and purified with Nucleospin RNA cleanup kit (Macherey-Nagel, Germany). The quality and integrity of RNA was checked on Agilent 2100 bioanalyzer (Agilent Technologies, California, USA). The sequencing library was constructed with SMRTbell Express template Preparation Kit 2.0 (Pacific Biosciences, California, USA) and Sequel II binding kit 2.1. The fragment profile of library was ascertained with the FEMTO pulse system (Agilent Technologies, California, USA). The library was sequenced on PacBio Sequel II system in CCS/HiFi mode