GSM7720802: PC-3 cells, (sh-miR93#1) shRNA with 5'-CUCGAG-3' loop, 3 replicate [mRNA-seq]; Homo sapiens; RNA-Seq
According to the manufacturer's instructions, the total RNA was isolated from about $1 \times 10^6$ cells using miRNeasy Mini Kit (Qiagen, Germany). All RNA samples were treated with DNase I during the isolation procedure. The RNA yield was determined by UV absorbance using a NanoDrop 1000 spectrophotometer (Peqlab, Germany). The RNA quality was assessed by analyzing the ribosomal RNA integrity number (RIN) on an Agilent 2000 Bioanalyzer using the RNA 6000 Nano kit (Agilent Technologies, USA). The RIN values of the isolated RNA samples were 9.5 and higher. According to the manufacturer's instructions, libraries for mRNA sequencing were prepared from total RNA samples using the MGIEasy RNA Library Prep Set (MGI, China).