GSM8142318: Orthochromatic, Luciferase-shRNA_rep2, RNA-seq; Homo sapiens; RNA-Seq
RNA was extracted from FACS-sorted orthochromatic erythroblasts following luciferase control and DNMT1 knockdown. Cells (1 × 106) were collected and washed twice with PBS. RNA was extracted from cell cultures using RNA extract kits (cat.no.74104, Qiagen) according to the manufacturer's instructions. RNA was extracted and used as the cDNA input, the cDNA library was prepared using a Truseq DNA library kit (Illumina), and the library was sequenced on a sequencing platform (HiSeq X Ten, Illumina).