Metagenome of 0.2-3 micron plankton from MGL1704 cruise in the North Pacific Ocean
Seawater was passed through 100 to 200 micron nitex prefilter followed by sequential filtering through 3.0 and 0.2 micron pore size filters. DNA from cells collected on the 0.2 micron filter was extracted using phenol:chloroform following the addition of T. thermus gDNA as an internal standard and enzymatic cell lysis. DNA was fragmented to ~600 bp and used to construct Nextera DNA Flex libraries. Metagenomic libraries were sequenced with the Illumina NovaSeq 6000 sequencing platform with S4 paired-end (2 x 150) chemistry.