GSM8240276: BrUseq_STK19-KO 12J3h; Homo sapiens; OTHER
Immediately after BrU pulse-labeling the cells were washed with PBS and lysed in TRIzol reagent (Invitrogen) and BrU-containing RNA was isolated as previously described (Van der Weegen et al.,2021, Nat Cell Biol; Andrade-Lima et al., 2015, Nucleic Acids Res; Van den Heuvel et al., 2021, Nat Commun) cDNA libraries were made from the BrU-labeled RNA using the Illumina TruSeq library kit and paired-end 151 bp sequenced using the Illumina NovaSeq platform at the University of Michigan DNA Sequencing Core.