Plasmodium falciparum amplicon-based short-read sequencing
Total DNA was extracted from dried blood spots without prior determination of parasite positivity. Short-read sequencing on an Illumina MiSeq was conducted after nested PCR reactions that amplified highly polymorphic regions within two genes: csp (Pf3D7_03_v3: 221419..221637) and sera2 (Pf3D7_02_v3: 320743-321001).