GSM8395208: LAD_scRNAseq_1; Homo sapiens; RNA-Seq
Left and right anterior descending arteries were washed, minced, and digested then filtered using cell strainers. Following a dead cell removal step, single-cell suspensions were counted and loaded onto a Chromium Controller (10x Genomics). Libraries were prepared using the Chromium Single Cell 3' Library v3 reagent, Gel Bead and Multiplex Kit and Chip Kit (10X Genomics; 1000268, 1000215 and 100127, respectively)