GSM7814160: sh.IP; Homo sapiens; RIP-Seq
The MeRIP assay was performed according to the protocol of RNA Immunoprecipitation Kit (GS-ET-006, Cloudseq, Shanghai, China). Cells cultured in 15cm dishes were collected with PBS, then scraped with RIP lysis buffer which containing protease inhibitor and RNase inhibitors Both the input without immunoprecipitation and the IP samples were used for MeRIP-seq library generation using NEBNext® Ultra II Directional RNA Library Prep kit (New England Biolabs)