GSM7498459: WT H3K27me3 high salt rep 1; Homo sapiens; OTHER
CUT&RUN was performed according to the CUT&RUN Assay Kit protocol (Cell Signaling Technology) with the following modifications. Cells were detached with Accutase (Sigma). 200,000 cells were collected per experiment and pelleted by centrifugation for 5 minutes at 600 x g. Beads were incubated in primary antibody on a nutator overnight at 4°C. MNase digestion was carried out at 0°