WES of H. sapiens: colorectal cancer tissue
Genomic DNA was isolated from FFPE tumor tissue of colorectal cancer patients using AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Hildesheim, Germany). 200 ng of DNA were fragmented using the SureSelect Enzymatic Fragmentation Kit (Agilent, Santa Clara, CA), libraries were prepared using SureSelect XT Low Input for Illumina Kit and SureSelect Human All Exon V7 probes set (both provided by Agilent) according to manufacturers protocol with the following modification. Samples were pooled for hybridization capture reaction multiplexing 8 samples and using total input 1500ng of DNA per reaction.