GSM6788439: pp1; Mus musculus; RNA-Seq
Thymi were harvested and digested in Collagenase D for 30 min, while peyer's patch cells did not undergo collagenase treatment. Single cell suspensions of both cell types were stained with antibody and sorted using a FACS ARIA II. RNA from sorted cells was isolated using the Qiagen Rneasy Micro kit per the manufacturer's instructions. RNA libraries were generated using Clontech Stranded Total RNA Pico Mammalian v2 and sequenced  on a NextSeq instruemnt (Illuminia)