DNAseq of Acinetobacter baumannii
DNA libraries were prepared from extracted gDNA using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB). DNA was purified and size selected using Ampure XP beads (Beckman Coulter) and quantified using Quant-it PicoGreen dsDNA assay (Thermo Fisher). The quality and size distribution of the DNA was assessed on a Fragment Analyzer using the HS NGS Fragment Kit (Agilent). The pooled samples were then sequenced on a NovaSeq6000 (Illumina) as PE250 by the McGill Genome Center (https://www.mcgillgenomecentre.ca/).