Fe 1
This project is based on the Ilumma novae / HiSe high-throughput sequencing platform, using the whole genome Wufa Whole Genome Shotgun WG strategy. The extracted metagenomic total DNA or macrotranscribed mRNA was used as a template to synthesize cDNA double strands, which were randomly interrupted into short fragments, and an insertion fragment library of appropriate length was constructed. These libraries were subjected to double-end Paired-nd ( PE ) sequencing to construct a library for each sample.