GSM6699507: Cep112-/- Input; Mus musculus; RIP-Seq
The cells were treated with cell lysis buffer.  The 10% lysis sample was stored and named “input”, and 80% was used in immunoprecipitation reactions with anti-Cep112 antibody and named “IP”, and 10%  was incubated with rabbit IgG (Cell Signaling Technology) as a negative control and named “IgG”， respectively.  The RNA of input and IP was extracted using TRIzol reagent (Invitrogen, Invitrogen, cat.  NO 15596026). The stranded RNA sequencing library was constructed by using KC-DigitalTM Stranded mRNA Library Prep Kit for Illumina® (Catalog NO.  DR08502, Wuhan Seqhealth Co., Ltd.  China) following the manufacturer's instructions.  The kit eliminates duplication bias in PCR and sequencing steps, by using a unique molecular identifier (UMI) of 8 random bases to label the pre-amplified cDNA molecules.  The library products corresponding to 200-500 bps were enriched, quantified, and finally sequenced on the DNBSEQ-T7 sequencer (MGI Tech Co., Ltd.  China) with the PE150 model.