GSM6914447: Mammary adenocarcinoma, database ID 20-078-04 [HET.19]; Mus musculus; RNA-Seq
Total RNA was isolated from tumour tissue using the RNeasy Minikit (QIAGEN) according to the manufacturer's protocol. Samples for RNAseq analysis underwent an on-column DNase I digestion step for genomic DNA removal prior to further processing. Total RNA quality and quantity was assessed using Agilent 4200 TapeStation and hsRNA or RNA ScreenTapes (Agilent Technologies). mRNA was isolated from 50ng of total RNA (RIN value >7) using the NEBNext® Poly(A) mRNA magnetic isolation module, (New England BioLabs, NEB, #E7490) and the sequencing libraries were prepared using the NEB® Ultra™ II Directional RNA Library Prep Kit for Illumina®