Illumina paired end sequencing
Leiomyosarcoma
Exome capturing was performed using xGen Exome Research Panel v1.0 based on standard protocols. Briefly, 550 ng genomic DNA was fragmented into 350 to 700 bp insert sizes using the Diagenode Bioruptor Pico system, according to the KAPA Hyper Prep Kit (KR0961-V6.17) protocols for library construction. A volume of 300 ng per library DNA from 12 samples was normalized and combined into a single pool for downstream target enrichment through hybridization with the capture probes of targeted regions. Paired-end sequencing (2 x 151 bp) was performed using Illumina NovaSeq6000. Sequence reads were aligned to human genome build 37 (hg19) by BWA (version 0.7.10) and samtools (version 0.1.19). Duplicated reads were marked from the BAM files using Picard (version 1.73). These BAM files were recalibrated by Genome Analysis Toolkits (version 3.8-1). Key genes involved in DNA repair analysed in this study was extracted in the final BAM files.