GSM6697764: Distal_Colon_WT_R20291-3; Mus musculus; RNA-Seq
Excised tissue was immediately placed in RNAlater on ice. Next day, tissues were sashed with sterile PBS then RNA was extracted by disrupting tissue in TRIzol, then precipitating RNA with addition of chloroform. Contaminating DNA was depleted with TURBO DNA-free kit, then RNA was cleand with Monarch RNA Cleanup Kit. Eukaryotic ribosomal RNA was depleted with NEBNext rRNA Depletion kit, then library was synthesized with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina. Multiplex oligos wer added using the Unique Dual Index Primer Pairs Set 3 from NEB.