Dynamics of a Chlainomonas sp. (Chlamydomonadales, Chlorophyta) algal bloom on Bagley Lake, North Cascades, WA
We submitted DNA to the University of Minnesota Genomics Center for amplicon library prep and sequencing of the V9 region of 18S rDNA using MiSeq Illumina 2 x 300 bp chemistry. Amplicons were generated for the V9 region of the 18S rRNA region using Earth Microbiome protocols  (https://earthmicrobiome.org/protocols-and-standards/18s/) and primers (Amaral-Zettler et al. 2009, Stoek et al. 2010) and the ITS2 region (Segawa et al. 2018).