GSM6697913: Synovial macrophage, sham surgical control, 12-week post surgery, rep 4; Rattus norvegicus; RNA-Seq
RNA was collected with TRIzol and purified using the Direct-zol RNA MicroPrep Kit (Zymo Research, R2062) as per the manufacturer's protocol. RNA was analysed using a Qubit 1.0 fluorimeter (Thermo Fisher Scientific) using the Qubit RNA high sensitivity kit (Thermo Fisher Scientific, Q32852); 500ug of RNA with RNA integrity numbers (RIN) of 7.8-9.3 were used for sequencing. RNA libraries for RNA-seq were prepared using the Illumina stranded total RNA prep kit with Ribo-Zero plus following the manufacturer's protocols.