Metagenome of Soil
The cultures were incubated in a 29 oC incubator for four weeks, monitored at 24 hours, 72 hours, and then once a week for four weeks using dark field microscopy (DFM). After four weeks of incubation and monitoring, 1 mL from each culture presumptively identified to contain Leptospira-like bacteria was collected, and DNA was extracted. A composite of positive samples of culture and soil was used to reduce the cost of testing. Extracted DNA was purified using SparQ PureMag Beads. Native barcoding genomic DNA Kit SQK-LSK 109 combined with EXP-NBD104.