GSM6569636: CUT&RUN, Tle3, Tem (≥30 dpi), 1; Mus musculus; OTHER
CD8+ T cell subsets were crossed-linked with formaldehyde, and then incubated with Tle3 antibody overnight, followed by incubation with Protein A/G-MNase, calcium-mediated MNase activation. The reaction was stopped and then genomic DNA fragments were extracted. The DNA segments from Tle3 Cut&Run were end-repaired and ligated to indexed Illumina adaptors, followed by amplification by PCR with a low number of cells (18-21 cycles). The resulting libraries were seqeunced with Illumina Hiseq-X platform.