GSM6637137: Sample_PC3_EGFP_2_IGO_13684_B_2; Homo sapiens; RNA-Seq
PC3 and 22Rv1 prostate cell lines were infected with Lentivirus with mutated ETV4: ETV4AAA-IRES-eGFP, wild type ETV4: ETV4WT-IRES-eGFP, and control vector IRES-eGFP by MSCV-IRES-GFP vector ((Addgene #20672)). After 1 week Lentivirus infection and transgene overexpression, live cells were sorted out by flow cytometry as DAPI negative and EGFP positive. Total RNA were extracted and library was built and sequenced by Poly A enrichment Whole transcriptome sequencing, NextSeq 500 Illumina sequencing instrument according to manufacturer's specifications. For each sample, 40~50 million reads were acquired. Cells were directly processed with 10X genomics Chromium Single Cell 3' GEM, Library & Gel Bead Kit v3 according to manufacturer's specifications. For each sample, 200 million reads were acquired on NovaSeq platform S4 flow cell.