GSM8395457: SI-214 vehicle replicate 2; Homo sapiens; RNA-Seq
RNA was extracted from the cells using Trizol (Invitrogen, 15596026) following the manufacturer's instructions. The RNA was precipitated using isopropanol, washed with 70% ethanol and resuspended in water. The RNA (100 ng) was then converted into a cDNA library using the SMART-seq mRNA LP (with UMIs) kit (Takara, 634762) following the manufacturer's instructions. The quality of the library was assessed using a High Sensitivity D5000 ScreenTape assay (Agilent) and then the cDNAs were sequenced using a NovaSeq 6000 with S1 Flow Cells (paired end, 2x50 bp).