RNA-Seq of WT saccharomyces cerevisiae during lucose starvation for 10 h
The total RNA was quantified with a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, USA), and the integrity of RNA was detected with Agilent 2100 Bioanalyzer system (Agilent Technologies, USA). The RNA-seq libraries were constructed by using an equal amount of high-quality total RNA for each sample, and each RNA-Seq library was constructed by NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, E7770). The products of the RNA-seq libraries were sequenced with a HiSeq 4000 system (Illumina, San Diego, CA).