Nanopore-sequencing and de novo genome assembly of the Talaromyces coalescens type strain CBS 103.83
Genomic DNA was subjected for library preparation using the SQK-LSK110 kit followed a single sample run on an R9.4.1 flow cell; to improve the coverage an additional library preparation was done using the SQK-NBD114.24 kit that was analyzed onto an R10.4.1 flow cell. Basecalling was done using the GridION build-in Dorado basecaller. FASTQ reads with a Q of 10 or higher were collected and QC processed using 'chopper' to select reads of 1100bp or longer, thereafter 50bp of both sides was removed followed by further downstream analyses.