GSM7498214: P13-Sham-vehicle; Mus musculus; RNA-Seq
The heart tissues were manually divided into three zones (infarct zone, border zone and remote zone) and then digested with collagenase type II enzyme at 37  C for 30 min to generate single-cell suspensions. F4/80/CD45+ cardiac macrophages in infarct zone and border zone were collected and subjected to FACS sorting for CD45+/F4/80+ cells. RNA libraries for RNA-seq were prepared using Genomics Library Prep Kit following manufacturer's protocols.