GSM6735101: RNAPII ChIP-seq mESC Replicate 3; Mus musculus; ChIP-Seq
Cells were crosslinked with formaldehyde for 2.5 minutes and quenched with glycine. After washing, cell pellets were resuspended in sonication buffer and sonicated using the Qsonica Q800R3 system. Chromatin was pre-cleared with Protein A agarose and incubated with the indicated antibody overnight. Protein A agarose beads were added and allowed to bind antibody-epitope complexes. IPs were washed extensively and eluted twice. DNA was purified using phenol/chloroform. ChIP-seq libraries were generated using the NEBNext Ultra II DNA Kit (New England Biolabs), following manufacturer's instructions.