GSM8284505: SUDHL4_LacZ_RGFP5_2; Homo sapiens; RNA-Seq
Total RNA was isolated RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions. Samples were quantified by Qubit RNA Kit (Life Technologies) and quality was assessed by Agilent Bioanalyzer. All samples had RIN ≥ 9.4. Libraries were prepared with 100 ng of total RNA using Stranded Total RNA prep with Ribo-Zero Plus kit (Illumina) with 13 cycles of amplification used. Final cDNA libraries were size validated using Agilent Bioanalyzer or Tapestation and concentration validated by qPCR (Kapa Biosystems/Roche). All libraries were normalized and pooled together, denatured with 0.2 N NaOH, and diluted to a final concentration of 250 pM.