16S rRNA gene amplicon of silage
Leftover TMR produced with acid-treated silage and dry barley in the 4th period. The DNA extraction was performed as described by Yu and Morrison (2004). For bacterial amplicon sequencing, universal primers targeting 16S rRNA gene V4 region were used (Caporaso et al., 2011). Libraries were prepared and sequenced on Illumina MiSeq platform using 2 x 250 bp chemistry.