GSM8354238: WT_377; Mus musculus; RNA-Seq
Caudal NP tissues from wild type and Opa1 conditional knockout mice (n=6 mice/genotype) were micro-dissected under a stereo microscope (Zeiss, Stemi 503) and immediately placed in RNAlater® Reagent (Invitrogen, Carlsbad, CA). Tissues were stored at -80°C until the time of RNA isolation, wherein they were homogenized with a Pellet Pestle Motor (Sigma Aldrich, Z359971), and RNA was extracted from the lysates using an RNeasy® Mini kit (Qiagen). Libraries for whole transcriptome RNA sequencing were prepared using the Stranded Total RNAseq with Ribo-zero Plus kit (Illumina, San Diego, CA) as per manufacturer's instructions starting with an input of 50 ng of RNA and 14 cycles of final PCR amplification.  Library size was assessed using the 4200 TapeStation and the DNA D5000 ScreenTape assay (Agilent, Santa Clara, CA).  Library concentration was determined using the Qubit Fluorometer 2.0 (ThermoFisher Scientific, Waltham, MA).