DNA ITS2 metabarcode of chaparral soil and DNA ITS2 metabarcode of test sample
DNA and RNA were extracted from field soil samples using Qiagen RNeasy PowerSoil Total RNA Kit and the Qiagen RNeasy PowerSoil DNA Elution Kit and amplified with plant universal primers (either rbcL, ITS2, or matK) using one-step RT-PCR (Qiagen) or regular PCR. Nucleotide concentrations were measured with Qubit and adjusted to equal molar concentrations before combining into one library before sequencing. Sample name designates the sample type. First number indicates plot origin from the field (e.g., 2, 3, 6, 7, or 9). Second letter (A or B)  indicates top 0-4 cm (A) or bottom 4-12 cm (B) soil level. Third number (1 or 2) indicates treatment with (1) or without (2) water incubation. Next letter indicates replicate (A, B, C, or D). Not all replicates successfully amplified. Next letter indicates DNA (D) or RNA (R). Last letter indicates barcode (I = ITS2, r = rbcL)