GSM7933234: MK3; Mus musculus; RNA-Seq
For the transcriptomic analysis, three brain tissues (hippocampus, cortex and cerebellum) from two groups (OBX and sham-operated) were used. Brains were dissected and stored at 800C until the RNA isolation. The extraction RNA was made using RNAzol RT (Molecular Research Center, Cincinnati, OH, USA) according to the company's protocol. The concentration and quality of RNA were determined via Qubit Fluorometer (Invitrogen) and an Agilent BioAnalyzer 2100, respectively, using RNA 6000 nano kit (Agilent technologies, Santa-Clara, CA, USA). For libraries RNA with Integrity Number (RIN), no less than 8 were taken. Illumina NEB Next Ultra II Directional RNA Library Prep Kit (NEB, Ipswich, MA, USA) was used for mRNA libraries preparation. The sequencing was performed on the Illumina NextSeq 2000 platform