GSM7932773: HepG2-HBV-Epi  scrambled siRNA rep 1; Homo sapiens; RNA-Seq
RNA was extracted from harvested cells using RNeasy mini kit, QIAGEN following manufacturer's instructions Libraries were prepared using Tru-Seq Stranded mRNA Library Prep kit for NeoPrep (Illumina, San Diego, USA) using 100 ng total RNA. Libraries were pooled and run as 75-cycle-pair end reads on a NextSeq 550 (Illumina) using a high-output flow cell.