GSM6394923: CD8T_6hr_L2HG_2; Mus musculus; RNA-Seq
Cells were washed with PBS once, and resusponded in TLC buffer freshly supplemented with 1% BME in the proprotion of 5 μL per 100 cells. Lysates were incubated at room temperature for 5 minutes and then stored at -80C . Total RNA was captured and purified on RNAClean XP beads. Polyadenylated mRNA was selected using an anchored oligo(dT) primer (5'AAGCAGTGGTATCAACGCAGAGTACT30VN-3') and converted to cDNA via reverse transcription. First strand cDNA was subjected to limited PCR amplification followed by Tn5 transposon based fragmentation using the Nextera XT DNA Library Preparation Kit.