GSM7720021: B. cereus, LF10, Rep a; Bacillus cereus; RNA-Seq
Total RNA preparation and on-column DNase digestion were performed using RNeasy® Mini Kit (Qiagen) according to the instructions of the manufacturer. RNA quality was tested via spectrophotometer and agarose gel electrophoresis. RNA purity was confirmed via PCR for 16 S rRNA. Random primed cDNA libraries were constructed according to the Illumina Genome Analyzer II protocol.