capp. RNA-Seq of Cupriavidus metallidurans AE104-EDTA
RNA was enriched for Cappable-seq RNA: Capping of  5' triphosphorylated RNA with 3'- desthiobiotin-TEG-guanosine 5' triphosphate (DTBGTP) (NEB) using the vaccinia capping enzyme (VCE) (NEB) for reversible binding of biotinylated RNA species to streptavidin. Capturing of biotinylated RNA species on streptavidin beads and elution to obtain the 5' fragment of the primary transcripts