PacBio sequencing of C. hepaticus strain GA81
Genomic DNA was extracted using MagAttract HMW DNA Kit (Qiagen, Hilden, Germany). The DNA sample was quantified using Qubit  dsDNA HS Assay Kit (Life Technologies, Carlsbad, CA), and the quality was evaluated using NANODROP 2000 (ThermoFischer Scientific, Waltham, MA) followed by an analysis ran into E-Gel SizeSelect 2% Agarose Gel (Invitrogen, Waltham, MA). The SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences, Menlo Park, CA) was used to prepare the genomic library for PacBio sequencing with BluePippin (Sage Science, Beverly, MA) size selection to target a library size of >7 kb. Genomic sequencing was perfomed on the PacBio Sequel II (Pacific Biosciences).