GSM7416874: 4G; Homo sapiens; RNA-Seq
T cells were activated and expanded from PBMCs with either Beads3/28 (αCD3 and αCD28 on commercial beads) with external IL-2 (30U/ml) or GO-APP3/28 (αCD3 and αCD28 on graphene oxide antigen presenting platform) in absence of external IL-2. T cells were collected 7 days after activation. RNA was extracted subsequently with miRNeasy Micro Kit (Qiagen). RNA libraries were prepared by the Technology Center for Genomics & Bioinformatics at UCLA using TruSeq Stranded Total RNA Sample Prep kit (Illumina) and sequenced with 50bp single end reads on an Illumina HiSeq3000.