GSM7846684: 1wk_SOX17KO; Mus musculus; RNA-Seq
Colon tumours were collected under the fluorescent microscope, minced using surgical scissors, and digested in a digestion buffer containing 500 U/ml Collagenase Type 1 and 20 μg/ml DNase at 37 °C for 40 min with gentle agitation. Tumours were then further dissociated with a gentleMACS Octo Dissociator on the tumour_imp1.1 setting, filtered through a 100-μM filter, and centrifuged for 5 min at 2000 rpm. 7-AAD-negative EpCAM-negative CD45-positive cells were sorted using FACS Aria II. 10x Genomics Single Cell 3' v3