GSM7501727: Female F2  (MaleDose2 x FemaleDose2) sample A; Mus musculus; RNA-Seq
RNA was extracted and purified using the TRIZOL technique (Invitrogen). RNA purity was confirmed by the A260/A280 and A260/A230 ratios of >1.8 and >2.0, respectively. RNA integrity of each sample was measured using an Agilent 2100 BioAnalyzer RNA libraries were constructed using the Stranded mRNA Prep Ligation kit (Illumina, San Diego, CA, USA) according to the manufacturer's instructions