RNA-seq of Roseburia intestinalis: monoculture 4h; replicate 3
Library preparation and sequencing of RNA-Seq libraries were carried out by the Genomics Core of KU Leuven. All samples were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs, #E7760L), with an initial amount of 100 ng total RNA in 12 l. Bacterial rRNA depletion was proceeded using the rRNA Depletion Kit (Bacteria) (New England BioLabs, #E7850L), and the residual mRNA was quality controlled and converted to the libraries that were sequenced on a NovaSeq 6000 (Illumina, San Diego, CA, USA) on a v1.5 S4_300 kit in XP mode. The resulting data of paired-end reads in fastq file format were obtained and demultiplexed before further analysis.