GSM8239702: wKD_rep2, dme ovary, chromRNA-seq; Drosophila melanogaster; OTHER
Chromatin-associated RNA was extracted as descirbed in (Chen et al 2016, PMID: 27292797). cDNA was synthesized using SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen). Reaction of tagmentation was performed in according to previously published protocol (Picelli et al. 2014 PMID: 25079858) using in-house produced Tn5 charged with oligonucleotides compatible with the Illumina system. Sequencing was performed on the Illumina HiSeq1500 platform.