GSM8240882: Mouse 2, pooled barcoded tissue Tregs, FB; Mus musculus; OTHER
Tregs were flow sorted on a BD Influx, Aria, Jazz or Fusion on the basis of CD4+Foxp3Thy1.1+ i.v.CD45- CD19-CD11b-CD8-F4/80-. Cells were labeled with Hashtag TotalSeq regents and loaded onto the 10x Chromium Controller. Sequencing was performed on an Illumina HiSeq. scRNA-Seq was performed using 10x Genomics 5' VDJ Single Cell Immune Profiling.