GSM7720215: 3T3_RNA_C3_batch3; Mus musculus; RNA-Seq
RNA-Seq libraries were prepared using Smart-Seq2 protocol (S. Picelli et al., 2014.). 1ng cDNA was fragmented by Tn5 and PCR was performed to amplify the library for 16 cycles using the following PCR conditions: 72 °C for 3 min; 98 °C for 30s; and thermocycling at 98°C for 15s, 60°C for 30s and 72°C for 3min; followed by 72 °C 5 min. After the PCR reaction, libraries were purified with the 0.4x-1.7x AMPure (Beckman) beads size selection and were subjected to next generation sequencing.