DNA library from saliva of individual HP1-S003 (Hair1.0 panel): Sample JK-SL4-S003
DNA from 500 µL of the saliva sample was extracted using the prepIT-L2P (DNA Genotek) reagent, following the manufacturer's instructions. Next, we prepared an Illumina library from 300 ng of DNA using the NEBNext Ultra II FS DNA Library Preparation Kit (NEB). Library was amplified and double-indexed using primers described in Kircher et al. (2012). We then prepared indexing PCR reactions as described in the NEBNext Ultra II FS DNA Library Preparation Kit and performed 4 cycles of PCR. Finally, we purified the spit library using a SPRI ratio of 0.9X and sequenced at UCSF on one lane of NovaSeq using the S4 2x150 kit.