GSM8187623: Wild Type Rep 1; Pseudomonas aeruginosa; RNA-Seq
RNA was purified using the RNeasy kit (QIAGEN) and residual DNA was eliminated with the RNase-free DNase Set Kit (QIAGEN). RNA-seq library construction and deep-sequencing were carried out by Sistemas Genomicos S.L. (Valencia, Spain). Ribosomal RNA was eliminated using the Ribo-Zero rRNA Removal Kit for bacteria (Illumina). RNA was chemically fragmented and directional (strand-specific) libraries were constructed using the Illumina mRNA Sequencing Sample Preparation Kit (TruSeq). Sequencing was performed using the Illumina HiSeq 2500 system (Illumina).