Smart-seq2 based profiling of CD4 T cells in individuals living with HIV-1
Following sorting of single p24+ and p24- cells into lysis buffer, cDNA was generated from each cell using a Smart-seq2 protocol. Next, cDNA was preamplified for 24 PCR cycles. Amplified cDNA products were used for library construction using the Nextera XT DNA Library Preparation Kit from Illumina. Pooled libraries were sequenced on an Illumina NextSeq 500.