CRISPR
Cells with incorporated single guide RNA library TKOv3 are treated with doxycyline to express NLRP3 R260W and subsequent induce cell death. After dox treatment, surviving cells were expanded for 1 week and purified using a live/dead MACS separation kit. Isolation of genomic DNA and PCR amplifications according to protocol: https://www.addgene.org/pooled-library/moffat-crispr-knockout-tkov3/.