Genomics of carbapenem-resistant K. pneumoniae outbreak strains from infants at Tembisa hospital
Briefly, your gDNA samples were fragmented using an enzyme based approach (part of NEBs NEBNext Ultra II kit work flow). Resulting fragments were purified (size selected), end-repaired and an Illumina specific adapter sequence was ligated to all fragments. Following quantification, the samples were individually indexed and a second size selection step was performed, using AMPure XP Beads. The libraries were quality controlled on a DNA chip (Agilent 2100 Bioanalyzer) and then sequenced on Illuminas MiSeq platform, using a MiSeq v3 (600 cycle) kit according to the manufacturers protocol.