GSM6395536: Walnut_11W_pellicle rep1; Juglans regia; RNA-Seq
Total RNA was extracted using RNeasy Plus Mini Kit (Life Technologies). Samples were then subjected to poly(A) purification using oligo-dT beads (Life Technologies) followed by rRNA removal using Ribo-Zero Kit (Epicenter) according to the manufacturer's instructions. RNA integrity was measured using 2100 RNA Nano 6000 Assay Kit (Agilent Technologies). Library construction were done using TruSeq® Stranded mRNA Library Prep Kit following the manufacturer's instructions (Illumina). 16 paired-end libraries were constructed and sequenced according to the Illumina HiSeqTM platform sequencing protocols.