GSM6944042: HEK293 cells, SAFB1 KD, rep3; Homo sapiens; RNA-Seq
Total RNA was extracted from cells with Quick-RNA MicroPrep kit (Zymo). Polyadenylated RNA was then isolated using Dynabeads™ mRNA Purification Kit. Purification was done twice to increase purity. Strand-specific libraries were generated using KAPA Stranded RNA-seq Library Preparation Kit (Roche)