GSM7498846: Testis cells, Control; Mus musculus; RNA-Seq
Single cell suspensions of control and EPAS1-cKO testis cells were created from n=2 mice each. The undifferentiated spermatogonia population was enriched using α6-integrin MACS beads. n equal number of cells from each of the biological replicates was combined in a single 'control' or 'EPAS1-cKO' tube.Live cells were loaded into a Chromium Controller (10X Genomics, Inc.). Single-cell cDNA libraries were generated using v3 chemistry according to the manufacturer's protocol (10X Genomics, Inc.).