GSM7498054: APC_856, APCmin/+, tyramine, colon, healthy; Mus musculus; RNA-Seq
Lysis buffer (Qiagen) was directly added to the tissue and RNA was extracted using the miRNeasy Micro Kit (Qiagen). RNA was treated with RNase-Free DNase Set (Qiagen) followed by RNA clean-up. RNA sequencing analysis was performed by Imperial BRC Genomics Facility (Imperial College London). Library was prepared using total RNA for cells.