GSM7416799: Cd_50 [R50-329_S8_L001]; Mus musculus; RNA-Seq
Placentae were isolated at e18.5, weighed, snap-frozen immediately and stored at −80°C. Frozen tissues were homogenized in lysis buffer and RNA was isolated via AllPrep DNA/RNA/miRNA kit (Qiagen). cDNA libraries for Illumina sequencing were constructed via the NEBNext Ultra Directional RNA Library Prep Kit (NEB) and NEBNext Multiplex Oligos for Illumina (NEB) according to the manufacturer's protocol. NovaSeq S4, utilizing a 150x2 bp paired end S4 sequencing