RNA_seq of Pichiapastoris
The mRNA fraction was purified from the total RNA using the MicroPoly Purist kit (Takara) according to the manufacturers protocol. The concentration and integrity of the mRNA was measured using a NanoDrop 2000 (Thermo Fisher Scientific) and the Agilent 2100 LabChip system (Agilent Technologies). The RNA was sheared, and reverse transcribed using random primers to obtain the cDNA, which was used for the cDNA library construction. Illuminam RNA-Seq libraries were subsequently performed using VAHTS Universal V6 RNA-seq Library P the SMARTer Stranded RNA Seq Kit (Vazyme Biotech Co. Ltd) according to the manufacturers instructions.