GSM6697337: Colon adenocarcinoma, STING-N153S, 477_CS; Mus musculus; RNA-Seq
CD45+/CD45- cells were pooled 1:1 Sorted cells were washed with PBS + 2% FCS Libraries were prepared using the Chromium Single Cell 3ʹ Reagent Kit v3.1 (10X Genomics, PN-1000269) and the 3' Feature Barcode Kit (10X Genomics, PN-1000262) All libraries from gene expression and feature barcoding were pooled and sequenced according to 10X Genomics' recommendations on an Illumina NovaSeq6000 system with a target read depth of 50000 reads/cell gene expression libraries and 5000 reads/cell for feature barcoding libraries