RNAseq of Mus musculus: aged males hippo 12
For library preparation, total RNA was processed using Illuminas TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA, USA) following the manufacturers instruction. Quality and quantity of the libraries were determined using the Agilent Bioanalyzer 2100 in combination with the DNA 7500 kit (Agilent). Sequencing was done on a HiSeq2500 in SR/50 bp/high output mode. Sequencing ended up with ~35 Mb reads per sample. Sequence data were extracted in FastQ format using the bcl2fastq software (Illumina).