S. commune in interaction with T. aggressivum CBS 100526, sampled from direct interaction zone
RNA was extracted with TRIzol (Thermofisher Scientific, USA) using the manufacturers protocol. Next, the sample was treated with DNAse I to remove genomic DNA and cleaned up using the RNA Cleanup protocol from the QIAGEN RNeasy Plant Mini Kit (QIAGEN, The Netherlands). Illumina libraries were generated for RNA-Seq and subsequently sequenced on the Illumina NextSeq 2000 platform in 2 x 50 bp mode (Utrecht Sequencing Facility; www.useq.nl).