GSM8283708: large1; Homo sapiens; RNA-Seq
Human AML Lin- immature cells were purified from primary human AML bone marrow using magnetic activated cell sorting kit with lineage negative magnetic beads (Miltenyi Biotec, 130-092-211) following manufacturer's instructions.  The purity reached more than 95%.For the scRNA-seq analysis of human AML lin- small and large cells,  small cells and large cells derived from bone marrow lin- cells of 4 AML patients were sorted using the CytoFLEX cell analyzer (Beckman Coulter Life Sciences, Indianapolis, United States) );.we pooled 4 sample from the small group in one tube and 4 sample from the large group in another tube..We then counted and resuspended the pooled cells at a concentration of 1000 cells/µL, targeting an estimated 6000 cells per library, following the instructions of the Single Cell 3ʹ Solution v3 Kit (10X Genomics). Single-cell libraries were constructed strictly according to the manufacturer's standard protocol. Each sequencing library is generated using a unique sample index. Libraries were sequenced on the Illumina NovaSeq 6000 System.