Effect of iron sources:Y4-bac
16S rRNA high-throughput sequencing method was applied to determine the composition of the microbial community in the AD sludge system. The samples were analyzed by Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai, China) with the Illumina MiSeq platform (Illumina, San Diego, USA). The V3-V4 region of 16S rRNA was amplified with specific primer sets. The primers for bacteria were 338F-806R, with the sequence of 5-ACTCCTACGGGAGGCAGCAG-3 and 5-GGACTACHVGGGTWTCTAAT-3, while the primers for archaea were 524F10extF-Arch958RmodR, with the sequence of 5-TGYCAGCCGCCGCGGTAA-3 and 5-YCCGGCGTTGAVTCCAATT-6.