GSM7814649: Barcoded samples of neurotypical and ASD diagnosed individuals; Homo sapiens; OTHER
Genomic DNA extracted from stool samples, using the PureLinkTM Microbiome DNA Purification Kit (ThermoFisher, Waltham, MA, USA) according to the manufacturer's instructions and following a 2-minute bead beating step (BioSpec, Bartlesville, OK, USA). Purified DNA was used for PCR amplification of the variable V4 region using the 515F and 806R barcoded primers following the Earth Microbiome Project protocol (Human Microbiome Project Consortium, 2012). equimolar amounts of DNA were pooled and sequenced using the Illumina MiSeq platform and MiSeq Reagent Kit V2 (500 cycles)