GSM6460065: AML, Tet2Tet3Pbrm1, Rep1; Mus musculus; RNA-Seq
Blasts from bone marrow of leukemic mice were processed into single cell suspension and enumerated from each biological replicate. RNA from roughly about 1 million cells were harvested using RNeasy Mini Plus kit (Qiagen). The resultant RNA were Dnase-treated and 200 ng RNA were used for subsequent enrichment and library preparation. RNA-seq libraries were prepared from poly-A-enriched mRNA using a KAPA mRNA HyperPrep Kit (Roche) and sequenced on an Illumina Novaseq.