GSM8395488: LA-Tg endothelial cells, microRNA, replicate 3; Mus musculus; miRNA-Seq
RNA extracts were prepared using miRNeasy Mini Kit (Qiagen 217084) For mRNA (with parallel isolation of microRNA libraries) from endothelial cells polyA enrichment was performed for mRNAs from 100 ng total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module according to the manufacturer's instructions. Library preparation of polyA+ enriched RNA was performed with the NEBNext UltraII Directional RNA library Kit from Illumina. microRNA libraries from endothelial cells were prepared from 100 ng total RNA with the QIAseq miRNA library Kit according to the manufacturer's instructions small RNA libraries from supernatants of endothelial cells and mouse plasma were prepared using CleanTag™ Small RNA Library Preparation Kit Catalog # L-3206.