GSM6597928: nucleus_ml240_ctrl3; Homo sapiens; RNA-Seq
iPSNs underwent subcellular fractionation into nuclear and cytoplasmic compartments using the Ambion PARIS kit cell fractionation buffer, following the manufacturer's general protocol and an 8M urea nuclear lysis buffer (50mM Tris-HCL (pH8), 100mM NaCl, 0.1% SDS, and 1mM DTT). Paired-end poly(A)+ selected reverse stranded RNA sequencing libraries were prepared from nuclear and cytoplasmic fractions using the NEBNext® Ultra II Directional RNA Library kit for Illumina®, with 500ng of total RNA as input.