GSM7500659: 1BR3 WT rep 1; Homo sapiens; RNA-Seq
Pellets were harvested by scraping cells in ice-cold PBS following by centrifugation at 7,500 x g for 10 minutes. Pellets were resuspended in 500µL TRIzol reagent (Invitrogen) and total RNA was extracted using a Direct-zol RNA miniprep kit (Zymo Research) according to the manufacturer's protocol. RNA concentration and quality was confirmed with the Agilent High sensitivity RNA ScreenTape, using the Agilent 4150 TapeStation System. Library preparation was performed by Novogene Corporation Ltd, using the Novogene NGS Stranded RNA Library Prep Set (PT044) and an in-house protocol for 250~300 bp insert strand specific library preparation. 50 million 150bp paired-end stranded reads were sequenced on an Illumina NovaSeq 6000 using an S4 flow cell. RNA-seq with rRNA removal to include ncRNAs and lncRNAs