RNA-seq of Sorghum bicolor developing grain
For this study, endosperms and outer layers were separated using a Leica LMD (LaserMicroDissector) microdissector. Samples were collected in a 50l tubes containing cell lysis buffer. The tubes were then frozen in liquid nitrogen and stored at -80C till extraction. This protocol was carried out for each developmental stage and in triplicate. Total RNA were extracted following instructions of the kit (Macherey-Nagel NucleoSpin RNA extraction), and their quantity and quality were evaluated (4200 Tapstation, Agilent Technologies).