GSM6569714: LECs, Jun-cKO; Mus musculus; RNA-Seq
The  lymphatic endothelial cells (CD45–CD31+GFP+) were sorted from Prox1-CreER;Junf/+;Rosa26-mTmG (Control) and Prox1-CreER;Junf/f;Rosa26-mTmG (Jun cKO) mouse lungs at P0 (3 to 5 mice were pooled for each experiment group). RNA was isolated from 1 X 104 murine lung LECs using an Arcturus PicoPure RNA isolation kit (KIT0204, Applied Biosystems) following the instructions. The RNA was then ampliﬁed using an Arcturus RiboAmp HS PLUS RNA ampliﬁcation kit (KIT0525, Applied Biosystems) following the instructions. cDNA libraries were generated using the NEB Next Ultra II DNA library prep kits (E7645L, New England Biolabs) and adapters in VAHTS DNA Adapters set1/2 for Illumina (N801-01/ N802-01, Vazyme). cDNA libraries were subsequently sequenced by Illumina Hiseq 2500 with 79 read lengths.