GSM8187550: Patient_PB_PBMC_CmCH1; Homo sapiens; RNA-Seq
RNA was isolated using the GenElute™ Total RNA Purification Kit (Sigma-Aldrich) and transcribed into cDNA using random hexamer primers and Superscript III® (both Invitrogen). Amplicon-seq: IGH transcripts of the samples were amplified using the forward VH1-6 FR1 (BIOMED-2) primers and custom IGHG, IGHA and IGHM reverse primers (sequence available upon request). PCR products were purified and sequenced using 2x300 Illumina sequencing (Illumina).