GSM8004848: SW480_but_cnt_2; Homo sapiens; ATAC-seq
ATAC-seq was performed using Omni-ATAC as described in Corces et al. (Nat. Methods 2017). 50,000 viable cells were pelleted and resuspended in ATAC-Resuspension Buffer. Cells were resuspended in transposition mix containing TD buffer and transposase (Illumina Tagmented DNA enzyme and buffer kit, Ref 20034210) Final PCR reactions were purified using Zymo DNA Clean and Concentrator-5 Kit (cat # D4013) Corces et al. (Nat. Methods 2017)