GSM6398471: HCC cells, day 0 (no dox), rep 1; Mus musculus; RNA-Seq
RNA was extracted using QIAGEN RNeasy kit. RNA-Seq libraries were constructed by Novogene. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.