GSM8058265: Retinal organoid differentiated from hPSCs, Day6, LDN treated, Biological replicate 2; Homo sapiens; RNA-Seq
Total RNA was collected from untreated aggregates on day 6 and treated aggregates on day 8 using the Picopure RNA extraction kit (Life Technologies). mRNAseq was then performed upon these samples to identify transcriptional changes across all conditions using a NovaSeq 6000 sequencer at the Indiana University Center for Medical Genomics. RNA quality was determined with an Agilent Bioanalyzer 2011, with a RIN number of 7 or higher. cDNA library preparation included mRNA purification/enrichment, RNA fragmentation, cDNA synthesis, ligation of index adaptors, and amplification, following the KAPA mRNA Hyper Prep Kit Technical Data Sheet, KR1352 – v4.17 (Roche Corporation). Approximately 30M reads per library were generated.