GSM6788006: 4360; Mus musculus; RNA-Seq
Mice were perfused with PBS and livers were inmediatelly frozen. Total RNA was extracted and purified using a RNA isolation Kit (Qiagen) followed by DNAse treatment to remove genomic contamination using RNA MinElute Cleanup (Qiagen). rRNA was depleted from RNA samples using Ribo-Zero rRNA Removal Kit (Illumina). RNA libraries were with performed TrueSeq Small RNA Library preparation (Illumina).RNA libraries were sequenced for 45 cycles on Illumina HiSeq 2000 platform (1 x 75bp read length)