GSM8283604: A673 Timepoint 0 (T0); Homo sapiens; OTHER
Genomic DNA was extracted using NucleoSpin Blood L kit sgRNA-containing cassettes were amplified by PCR from genomic DNA samples. During the PCR, Illumina adapters and TruSeq indexes were added, the 270 bp PCR fragments were purified by SPRI beads.Detatailed protocol at https://weissman.wi.mit.edu/resources/IlluminaSequencingSamplePrep.pdf