RNA-seq of Pyrosoma atlanticum
The poly (A) mRNA was enriched with magnetic beads containing oligo (dT) to process RNA. The first strand of cDNA was synthesized using the mRNA fragment interrupted by interrupting agent as a template, the second strand synthesis reaction system is then configured to synthesize the second strand cDNA. The second strand of cDNA was purified with Ampure XP beads, and the sticky ends were repaired. The "A" base was added to the 3 end of the cDNA, and PCR amplification was carried out with manufacturer's instructions. Then, the Agilent 2100 Bioanalyzer system and the ABI StepOnePlus real-time PCR system were used to inspect the constructed library. The libraries were sequenced on an Illumina HiSeq X 10. The experiments were carried out using three replicates.