PacBio Isoseq of Sitodiplosis mosellana:  pooled adults and pooled 3rd instar larvae
Total RNA was extracted from either 100 3rd instar larvae or 20-30 adults using the Cytiva RNAspin Mini kit.  Each was quantified on a nanodrop spectrophotometer, then pooled in equal amounts (equal nanograms) prior to library generation.  Two libraries were generated using :  a library with standard size selection and another with selecting long reads.  These libraries were pooled 80% standard :20% long reads and sequenced (without barcoding) on the same chip.