16s amplicon: bioturbator burrows and adjacent surface sediments bacterial communties
Samples were sequenced using a MiSeq Sequencing Platform (Illumina). The bacterial 16S rRNA genes were PCR amplified using the 16S V1-V3 amplicon library prep primers 27F(5-AGAGTTTGATCMTGGCTCAG-3) and 519R(5-GWATTACCGCGGCKGCTG-3) with 2x300 base pair sequencing, to produce an amplicon size of 492 base pairs