Amplicon of Gallus gallus domesticus: ileal mucosal microbiota
DNA extraction performed using a Dneasy PowerSoil kit (Qiagen, Valencia, CA) and QIAcube instrument (Qiagen). Peformed 16S rRNA amplicon sequencing of the variable V3-V4 region. Pooled DNA library diluted to final concentration of 4 pM and mixed with PhiX control. 2 x 300bp.