Whole genome resequencing of Chenopodium quinoa
Genomic DNA from young leaves was extracted using a cetyltrimethylam-monium bromide (CTAB)-based protocol. The concentration and quality of the total genomic DNA were determined using a NanoDrop2000 Spectrophotometer (Thermo Fisher Scientific). DNA libraries (350bp) for Illumina sequencing were constructed for each accession according to the manufacturer's specifications. After DNA library construction, sequencing was performed on an Illumina HiSeq XTen platform by a commercial service (Biomarker Technologies, Beijing, China), with 150-bp read lengths. Raw reads were filtered based on the following criteria: pair-end reads with >10% 'N' bases; Reads, on which more than 50% of the bases have a quality score less than 30 (Phred-like score); Sequencing adapter. Finally, high-quality sequences were obtained for subsequent analyses.