Oregon Health and Science University SARS-CoV-2 Genomic Surveillance
RNA was extracted following the procedure for MagNA Pure Viral RNA extraction. The library was prepared by following the subsequent steps: anneal RNA, synthesize first strand cDNA, amplify cDNA, tagment PCR amplicons, clean up post tagmentation, amplify tagmented amplicons, pool and clean up libraries. The libraries were then quantified and normalized. The sequencing procedure is as follows: prepare reagent and sample sheet for sequencing, denature and dilute libraries for sequencing using Nextseq550, sequence libraries. Results were interpreted using performance characteristics of inclusivity and cross-reactivity.