Ont of Whitmania laevis
A long-read library (insert size, 20 kb) was constructed using the SQKLSK109 ligation kit. The purified library was loaded onto primed R9.4 Spot-On Flow Cells and sequenced using a PromethION sequencer with 48-h runs. Base-calling analysis of raw data was performed using the Oxford Nanopore GUPPY software.