DNA Sequencing of Nontox17 (SRRC 1588)
High quality genomic DNA was extracted using a QuickDNA Bacterial/Fungal MiniPrep Kit (Zymo Research, Irvine, California, USA). Freeze-dried mycelia was placed in a 2 ml tube containing glass beads with cell lysis solution. The tubes were placed in a Mini-Beadbeater-16 (Biospec Products, Bartlesville, Oklahoma, USA) for 3 min to pulverize the tissue. The steps following homogenization were carried out per Zymo kit instructions.