16S rDNA of equine Feces
Amplification and sequencing of the V3-V4 variable region the16S rRNA gene was performed commercially (Zymo Research). Briefly, a library was prepared using a commercially available 16S rDNA prep kit (Zymo Research quick-16S NGS Prep Kit), samples were barcoded and PCR primers for the V3-V4 hypervariable region of the 16S rRNA gene were used. Sequencing was performed on a MiSeq (Illumina) following the manufacturers guidelines.