Microbiome survey of the phoronid, Phoronopsis harmeri, from Bodega Bay, CA
For high-throughput amplicon library preparation and sequencing, samples were randomized and placed in 96-well plates to be sent to Zymo Research, Inc. via their ZymoBIOMICS Service. The 16S ribosomal RNA (rRNA) gene was amplified using the Earth Microbiome Project (EMP) 515F (Parada) and 806R (Apprill) primer set. Libraries were produced using the Quick-16S NGS Library Preparation Kit (Zymo Research, Irvine, CA). PCR products were quantified with qPCR and pooled together based on equal molarity. The pooled library was cleaned with Select-a-Size DNA Clean & ConcentratorTM (Zymo Research, Irvine, CA, United States), then quantified with TapeStation (Agilent, Santa Clara, CA, United States) and Qubit (Invitrogen, Carlsbad, CA, United States). The final library was sequenced on an Illumina MiSeq (Illumina, Inc., San Diego, CA, United States) with a v3 reagent kit (600 cycles) to generate 300 bp paired-end reads.