Metagenome of cold geyser Wallender Born
DNA extraction from filter samples via Powersoil Max kit of qiagen, library preparation via Westburg NGS DNA Library Prep Kit (cat. no. WB 9096) without PCR amplification. Insert size validation to be more than 400 bp. Sequencing with 2x 150bp paired-end reads on a NovaSwq500 platform to an approximate sequencing depth of 20 Gbp. Some samples' libraries were sequenced multiple times to get to that sequencing depth. Thats why some samples have multiple files. For analysis, the for/rev paired end read sets were concatenated, respecitvely, and treated as one sample.