illumine raw data
DNA samples qualified by electrophoretic detection were randomly broken into fragments with a growth of about 350 bp by Covaris ultrasonic breaker. DNA fragments after processing, using NEBNext  Ultra  DNA Library Prep Kitfor Illumina (NEB, USA) kit completes the preparation of the entire library through terminal repair, adding A tail, adding sequencing connector, purification, PCR amplification and other steps. After the construction of the library, first use Qubit 2.0 for preliminary quantification, dilute the library to 2ng/ul, and then use Agilent 2100 to detect the inserted fragments of the library. After the insert size meets the expectation, use Q-PCR to accurately quantify the effective concentration of the library, so as to ensure the quality of the library.