RNA-Seq of Mus Musculus: 18-month-old male mice cardiomyocytes administered C646
DNAse-treated total RNA was extracted from CMs using the Maxwell RSC simplyRNA Cells Kit (Promega, Madison, WI, United States). RNA-seq library preparation was performed with the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara Bio), according to the manufacturer's instructions. Ribosomal RNA was depleted using the RiboGone method included in the kit. Electrophoresis of the libraries on an Agilent 4200 TapeStation instrument showed average library size of 321391 bp.