PacBio data for Malus domestica 'WA 38' Genome
Approximately 10 grams of young leaf material was harvested from the Malus domestica WA 38 mother tree at the Washington State University and USDA-ARS Columbia View Research Orchard near Orondo, WA, USA and flash-frozen in liquid nitrogen. High molecular weight DNA was isolated using using a Circulomics Nanobind Plant Nuclei Big DNA kit (Baltimore, MD), with 4 g of input tissue and a 2 hour lysis. DNA was tested for purity via spectrophotometry, quantified by Qubit dsDNA Broad Range, and sized on an Agilent Femto Pulse. DNA was sheared with a Diagenode Megaruptor and size-selected to roughly 25 kb on a BluePippin. A PacBio sequencing library was produced using the SMRTbell Express Template Prep Kit 2.0, and CCS (HiFi) reads were produced on two 8M flow cells.