RNASeq of PK-1 or PANC-1 cells in 3D
Library preparation and sequencing was performed at the Wellcome Sanger Institute next-generation sequencing facility (Cambridge, U.K). PolyA purified opposing strand library kit was used, and 4 samples per lane were multi-plexed in 6 lanes on Illumina HiSeq 2000, 2x75bp, Paired-End reads. 75-125 million pairs were sequenced per sample.