RNA Seq of Xy
sample were taken from different location of anode or cathode. The DNA extraction was performed using E.Z.N.ATM Mag-Bind Soil DNA Kit (Omega Bio-Tech, GA, USA), according to manufacturer guidelines. The V4-V5 region of 16s rRNA gene were amplified using barcode primer ArBa515F (5-GTGCCAGCMGCCGCGGTAA-3) and Arch806R (GGACTACVSGGGTATCTAAT-3) that cover all of the bacterial and archaeal taxa