16S rRNA gene amplicon sequencing in Fermented raw milk
Amplicon libraries were prepared using the unidirectional fusion method. PCR of the V1V2 16S rRNA gene region was performed using primer NGS_ABCxF27 and NGS_trP1_355. The amplification was carried out as follows: 94 C for 2 min, followed by 18 cycles of 94 C for 30 s, 55 C for 30 s, and 68 C for 30 s. All amplicons were purified using AMPure XP beads with a bead-to-DNA ratio of 1.8.