miRNA-Seq of scrofa black epidermis
After the sample was tested, 1.5ug was used as the starting RNA sample volume to 6ul with water, and library construction was performed using the small RNA Sample Pre Kit kit. Because Small RNA has a phosphate group at the 5  ends and a hydroxyl group at the 3  ends, T4 RNA Ligase 1 and T4 RNA Ligase 2 (truncated) at the small RNA 3  and 5  ends, respectively, reverse transcription of cDNA, PCR amplification, screening the target fragment, and recycling the small RNA library. After the sample was tested, 1.5ug was used as the starting RNA sample volume to 6ul with water, and library construction was performed using the small RNA Sample Pre Kit kit. Because Small RNA has a phosphate group at the 5  ends and a hydroxyl group at the 3  ends, T4 RNA Ligase 1 and T4 RNA Ligase 2 (truncated) at the small RNA 3  and 5  ends, respectively, reverse transcription of cDNA, PCR amplification, screening the target fragment, and recycling the small RNA library.