BS-seq of P. vivax sporozoites: extracted salivary glands from A. dirus
Dissected from infected A. dirus mosquitoes at IPC and cryopreserved. Sporozoites were lysed and purified with phenol-chloroform. Each sample subjected to bisulfite conversion using the Epitect fast bisulfite conversion kit, and libraries prepared using the Accel-NGS methyl-Seq DNA library kit.