scRNA-seq of hiPSC-derived striato-nigral circuitoids: 80 days in vitro
For the scRNA-seq,  striatal organoids and striato-nigral circuitoids  were dissociated by 1 ml of trypLE (Life Technology Inc) for 30 min in the condition of 37C and 5% CO2 into single cell when differentiated in vitro for 30, 60, and 80 days. The cell debris and other aggregates were removed by additional low-speed centrifugation. Droplet-based single-cell RNA sequencing was performed using the 10X Genomics Chromium Single Cell platform, version 3. Approximately 5,000 cells were captured for each sample onto a Chromium Single Cell 3 Chip (10X Genomics, PN-120236), and the released RNA in each cell was barcoded through reverse transcription in individual gel beads in emulsions (GEMs).