Bacterial 16S metagenom of deep sediment layer 19-20 cmblf
The V2-V3 variable region of the bacterial 16S rRNA gene was amplified by PCR with  primers,containing rRNA-specific parts: B_V23F (5-AGTGGCGGACGGGTGAGTAA) and B_V23R (5-CCGCGGCTGCTGGCAC). The libraries were analyzed using Illumina MiSeq Standard Kit v.3 (Illumina) at the Core Centrum "Genomic Technologies, Proteomics and Cell Biology" (ARRIAM, Saint-Petersburg).