16S rRNA V4 region of negative control from sample Negative_control_Candace_6-10-18
The negative control was a sample tube in which no fecal material from a crane was added, but which went through the entire DNA extraction process along with tubes with samples from the cranes.  Then the V4 region of the 16S rRNA gene was amplified using the primers 515F and 806R.  Three independent PCRs were performed for each sample, which were then combined. Equimolar amounts of each sample were then pooled before sequencing on a 151 bp paired-end run of an Illumina MiSeq.