Whole exome sequencing of Mus musculus: Rrm1:+/Y285A tumor
Qualified genomic DNA samples are randomly sheared by the ultrasonic high- performance sample processing system (Covaris) into fragments with a main peak of about 200bp-300bp. Subsequently, repair the end of DNA fragment, add nucleotide A at 3' end, and ligate adapters at both ends. The library ligated with adapters is linearly amplified (LM-PCR) to poduce a hybrid library. Take an appropriate amount of hybrid library for capture and enrichment with exon chip, elute the unenriched fragments followed by amplification. The amplified products are subject to QPCR quality control, qualified products will be used for sequencing.