16S of fecal samples lung cancer patient
V3-V4 16S rRNA amplification following 16S-metagenomic-library-prep-reference guide of Illumina. Quality control of the size of the length distributions of the final fragments obtained in the library was performed using the Tape Station 4150 (Agilent technologies) and the final concentration of the library with Qubit 2.0 (Invitrogen by LifeTechnologies).Sequencing of the libraries is performed on the Miseq sequencer (Illumina) on a MiSeq Reagent Kit v3:600 cycles (Illumina) cartridge (2x300pb) for 600 cycles.