miRNA profile of early-stage MSC-EVs
RNA from extracellular vesicles of chorion-derived MSCs were isolated from a 24h culture condition media using the the SeraMir Exosome RNA Purification kit (System Biosciences, USA). The quality and quantity of small RNA was inspected with Bioanalyzer (Agilent, USA) and RNA was sent to Novogene (UK) for Small RNA-Seq on Illumina platform in 150 bp reads.