Illumina MiSeq sequencing of dsRNA for grapevine virus detection
We synthesized 23 cDNA samples using the same dsRNA materials that were used for dsRNAcD library preparation, according to the cDNA synthesis procedure described in the section on dsRNAcD library preparation. An Illumina Nextera XT DNA Library Preparation Kit (catalog number FC-131-1096) was used for this process, using 1 ng of double-stranded cDNA as input. Paired-end sequencing was carried out using the MiSeq Reagent Nano Kit v2 in combination with an Illumina MiSeq sequencer