Ion torrent sequence data: Endometrial cancer microbiota compared to healthy endometrium
RNA was isolated from Formalin-Fixed Paraffin-Embedded (FFPE) tissue samples using RecoverAll Total Nucleic Acid Isolation Kit (Ambion, life technologies). cDNA was synthesized from RNA isolated by reverse transcription reaction using PrimeScript RT Master Mix Samples (Perfect Real Time) (TaKaRa). The cDNA samples (5ng/ul) were amplified using primers encoding for the hypervariable regions V2-4-8 and V3-6, 7-9 of the bacterial 16S rRNA using the 16S Metagenomics Kit (Thermo Fisher Scientific). libraries were created and sequenced using the Ion 520 TM/ 530 TM Kit-Chef (Thermo Fisher Scientific) and the Ion 520 chip (Ion 520TM Chip Kit), using the Ion Chef and the Ion Torrent S5TM system platform.