16S sequencing of mouse gut microbiome: stool from mouse 334_N at baseline
DNA was extracted from stool pellets using the QIAGEN DNeasy PowerSoil Pro Kit, the V1/V2 variable region of the 16S gene was amplified using KAPA HiFi HotStart ReadyMix and the F27/R338 primer-pair (F27: 5'-AGAGTTTGATCCTGGCTCAG-3', R338: 5'-TGCTGCCTCCCGTAGGAGT-3'), pooled libraries underwent size selection using AMPure XP SPRI beads, and pooled libraries were paired-end (2x250 bp) sequenced across two MiSeq runs.