small intestine microbiome of mus musculus: zinc treatment to the SFB-colonized mice
Genomic DNA was extracted from ~50 mg of samples using a Quick-DNA Fecal/Soil Microbe Microprep Kit (Zymo Research) according to the manufacturers protocol. The V4/V5 region of the bacterial 16S rRNA gene was amplified using the Phusion High-Fidelity DNA polymerase (Thermo Scientific) with 518F/926R primer pair. The amplicons were sequenced on Illumina MiSeq 2  300 bp paired-end sequencing.