Human Whole Genome Sequencing, 2x150, Adapter Trimmed
Human DNA from cell line sample HG002 was obtained from Coriell Institute.  Linear NGS library construction was performed using a KAPA HyperPrep library kit (Roche) according to published protocols. Finished linear libraries were circularized using Element Adept Compatibility kit (catalog #830-00003). Final circular libraries were quantified by qPCR with the standard and primer set provided in the kit. Circular library DNA was denatured using sodium hydroxide and neutralized with excess Tris pH 7.0 prior to dilution. Denatured libraries were diluted to 8pM in hybridization buffer before loading onto the sequencing cartridge.   The processed library was sequenced on the AVITI System with 150 cycles for Read 1 and150 cycles for Read 2.