iGUIDE targeting MAPK8 in HEK293T: Nme2-C.NR
In brief, half million HEK293T cells were transfected with 1000 ng nuclease plasmid, 500 ng gRNA plasmid and 50 pmol double-stranded oligodeoxynucleotide (dsODN) using Lonza 4D system (Program CM-130). Cells were collected 3 days after nucleofection for genomic extraction. The genome library was prepared and subjected for sequencing.