MiSeq Illumina of biofilm on polystryene: Week 1 of incubation
The taxonomic composition of bacterial communities was determined by sequencing their DNA using a MiSeq Illumina platform (2x300bp paired-end) after PCR amplification of the V4 region of bacterial 16S rRNA genes using primers 515F and 806R. Bioinformatic processing and analyses were performed using QIIME 2 v2020.2 where reads were assembled, demultiplexed, and trimmed to salvage reads that had a median quality score above 25. PhiX reads and chimeric sequences were filtered using DADA2. Reads were then clustered into Amplicon Sequence Variants (ASVs) at 100% similarity and were classified against the Silva (version 138) database using a trained classifier.