16S rRNA seq for Homo sapiens: saliva sample
DNA was extracted using the DNeasy PowerSoil Kit (Qiagen, Hilden, Germany) according to the manufacturers instructions. The extracted DNA was quantified using a Quant-IT PicoGreen (Invitrogen, Waltham, MA, USA). Sequencing libraries were prepared according to Illumina 16S Metagenomic Sequencing Library protocols to amplify the V3 and V4 regions.