PacBio HiFi CCS DNA of Medicago truncatula accession HM078
The PacBio SMRTbell prep kit 3.0 was used to for library preparation following the manufacturers specifications, and long-read sequencing was performed on the Sequel II system with two 8M SMRT Cells running for a 30-hour period. SMRT Link v10.0 determined the long-read sequence data consisted of 5.4 million circular consensus sequencing (CCS) High Fidelity (HiFi) reads consisting of 65.04 Gb with a Phred-scaled quality score greater than Q20.