RNA-seq of Salmonella enterica serovar Heidelberg strain SX 244
The RNA sample was extracted using the Qiagen RNeasy Mini Kit and treated with the Ambion TURBO DNase kit to remove genomic DNA and the Illumina Ribo-Zero Plus rRNA Depletion Kit to deplete bacterial rRNA. The RNA library was constructed using the NEBNext Ultra II Directional RNA Library Prep Kit and sequenced on an Illumina Hiseq 3000 (150-cycle, single-end reads).