16S of gut rabbit metagenome: Cecum mucosa of rabbit 56, breed: GF, sampleID: GF7
Total bacterial genomic DNA was isolated from the content of the jejunum and cecum (n = 10 for each breed, NM and GF) by using the Easy Pure Stool genomic kit protocol (TransGen Biotech, Haidian District, Beijing, China) following the manufacturers instructions. The 16S ribosomal RNA (rRNA) gene was amplified using primers targeting the V3-V4 hypervariable regions according to the 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). Amplicons were cleaned with Agencourt AMPure XP (Beckman, Coulter Brea, CA, USA), and libraries were prepared following the 16S Metagenomic Sequencing Library Preparation Protocol (Illumina, San Diego, CA, USA). The libraries obtained were quantified by real-time PCR with KAPA Library Quantification Kits (Kapa Biosystems, Inc., Wilmington, MA, USA), pooled in equimolar proportion, and sequenced in one MiSeq (Illumina) run with 2  250-base paired-end reads.