Hypothalamic and testicular transcriptomes of ZIKV-infected BALB/c suckling mice
RNA sequencing of the hypothalamus and testes harvested from ZIKV-infected or control mice at 10 and 56 dpi was commercially conducted (LC-Bio Technology CO., Ltd., Hangzhou, China). Three to four individuals were in each group. In brief, after extraction of total RNA (Trizol reagent, ThermoFisher, 15596018), mRNA was purified by Dynabeads Oligo (dT) (Thermo Fisher, CA, USA). The mRNA was fragmented by Magnesium RNA Fragmentation Module (NEB, cat.e6150, USA) to create cDNA libraries. Real-Time PCR System was used to quantify and qualify the sample libraries. Finally, the cDNA libraries were sequenced using Illumina Novaseq 6006 (Illumina, Inc., USA).