WGS of Cronobacter sakazakii
The ES191 genome was sequenced using the Pacific Biosciences Sequel II platform (Novogene, China).The SMRTbell genome library was prepared as instructed by the SMRTbell prep kit 2.0 procedure and checklist (Novogene, China). The ES191 genome was barcoded using SMRTbell barcoded adapter plate 3.0 (Novogene, China), pooled with two other bacterial genomes, and sequenced in one SMRT (single-molecule real-time) cell, which was carried out with 120 pM on- plate concentration, Sequel II binding kit 2.0, diffusion loading type, chemistry 11.0