RNA-Seq of MDA-MB-231 cells: GFP, luciferase, rtTA, dox-inducible dSRF MRTF-A expression
MDA-MB-231 cells cultured in tissue cultured treated plates expressing GFP and luciferase were treated doxycycline to induce dSRF MRTF-A OX for 72 hours prior to RNA collection with the Qiagen RNeasy Plus Kit. Libraries were obtained starting for total RNA followed by library preparation using the Illumina Stranded Total Ligation (Ribo-Zero Plus) protocol. Sequencing was then preformed on the Illumina NextSeq2000.