RNA-Seq of MDA-MB-231 cells: GFP, luciferase, rtTA expression (Control)
MDA-MB-231 cells cultured on top of Matrigel expressing GFP and luciferase were treated doxycycline for 72 hours prior to RNA collection with the Qiagen RNeasy Plus Kit. Libraries were obtained starting for total RNA followed by library preparation using the Illumina Stranded Total Ligation (Ribo-Zero Plus) protocol. Sequencing was then preformed on the Illumina NextSeq2000.