Infant nasal microbiota, Sample BUG030, Healthy Control
Two nasal swabs were collected at the enrollment for each subject according to the World Health Organization (WHO) guidelines and stored in sterile plastic vials at -80C. Total bacterial DNA was extracted from nasal swabs using QIAamp UCP Pathogen Mini (QIAGen, Hilden, Germany) following manufacturer guidelines. Metabarcode sequencing analyses were conducted to explore the composition of bacterial nasal communities. The V3-V4 region of the rRNA gene was amplified according to the the 16S Metagenomic Sequencing Library Preparation Protocol (Illumina, San Diego, CA, USA). Then, libraries were normalized, pooled and, subsequently, sequenced on an Illumina MiSeq platform by  2  300bp paired-end run.