WGS of Homo sapiens: tRCC
For linked-read genome sequencing, high molecular weight DNA for library construction was prepared using 10X Chromium protocol (10X Genomics). 10X Genomics WGS Library Construction DNA samples were normalized for each sample. Library fragment sizes were determined using the DNA 1000 Kit and 2100 BioAnalyzer (Agilent Technologies) and quantified using qPCR (KAPA Library Quantification Kit, Kapa Biosystems). The resulting libraries were sequenced to ~30X coverage on an Illumina HiSeqX platform, using paired 151 bp reads with a single 8 bp index read.