Amplicon sequencing of ITS2: Veronica austriaca subsp. jacquinii
Target ITS2 region was amplified using ITS3 (5 GCATCGATGAAGAACGCAGC 3) and ITS4 (5 TCCTCCGCTTATTGATATGC 3) primers. Library preparartion was outsourced to Novogene and sequenced using PE250 strategy.