Iso-seq of Pyrostremma spinosum
By using the SMARTer PCR cDNA Synthesis Kit (Clontech, Mountain View), full-length cDNA was synthesized from the purified total RNA, and large-scale PCR was performed to generate more double-stranded cDNA templates. Then, the PacBio Template Prep Kit (PacBio) was used to create the SMRTbell libraries. Subsequently, the Pacific Sequel platform was used for SMRT sequencing.