16S rRNA gene amplicon sequencing of weathered bedrock material: in-situ
Microbial 16S rRNA genes were amplified using primers 515F and 806R targeting the V4 region. Nextera 2 nd step PCR incl. pooling. Sequencing on Illumina MiSeq, v3, 2x300 bp. Primers were trimmed with cutadapt.