RNA-Seq of Klebsiella Pneumoniae kgh3 mutant: Planktonic
Cells were collected separately and cells were lysed by homogenizer before total RNA was extracted using RNAprep Pure Cell/Bacteria Kit (TIANGEN) according to the manufacturer's instructions. The Agilent 2100 Bioanalyzer was used to check RNA integrity and concentration. Qualified total RNA was processed for Illumina sequencing and 150 bp paired-end reads were generated, and sequencing data were further processed to obtain clean data for high quality analysis.