SELEX based identification of aptamers against SARS-Cov-2
After 10 rounds of SELEX, aptamers were cloned in pET-32 Xa/LIC vector using the ligation-independent cloning (LIC) technique. Then, ligated aptamer-pET-32 Xa/LIC recombinant product was transformed into E. coli DH5 competent cells and plated on SOC agar medium containing ampicillin (40g/ml). The plated cells were incubated overnight to check the positively transformed colonies. Finally, recombinant plasmids isolated from different transformed colonies were sent to Eurofins Genomics India Private Limited, India for sequence analysis of the screened aptamers.