RNAseq of sample MUT_DMSO replicate 1
Total RNA was extracted using RNeasy Mini Kit (Qiagen ID: 74104) complemented with on-column DNAse digestion with the RNAse-free DNAse set (Qiagen ID: 79254) according to the manufacturers instructions. RNA was quantitatively and qualitatively evaluated using NanoDrop 2000c (Thermo Fisher Scientific) and Agilent Bioanalyzer 2100 (Agilent Tecnologies), respectively. RNA-seq libraries were prepared from 1 g of total RNA, using the Illumina's TruSeq Stranded Total RNA Sample Preparation Kit (Illumina, San Diego, CA, USA), according to the manufacturers protocol. cDNA libraries were qualitatively checked on the Bioanalyzer 2100 and quantified by fluorimetry using the Quant-iTTM PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) on NanoDrop 3300 Fluorospectrometer (Thermo Fisher Scientific). Sequencing was performed on NextSeq500 platform, generating for each sample almost 100M of 100bp paired-end reads.