V3V4 region on 16S rDNA gene amplicon from envrionmental DNA extracted from anaerobic digestion sludge
DNA was isolated using Fast DNA Spin Kit for Feces (MP Biomedicals, Illkirch, France). V3V4 hypervariable region of 16S rDNA gene was amplified using Bakt_341F/Bakt_805R primer pair (Muyzer et al., 1993; Mizrahi-Man et al., 2013).