RNA-Seq of Champsocephalus gunnari: adult female egg
For RNA extraction, 10 tissues comprising brain, egg, esophagus, eye, fin, heart, kidney, muscle, skin, spinal, spleen, and stomach were dissected and then stored in RNAlater (Ambion, Inc., Austin, TX, USA) solution. Total RNA was extracted from each tissue using the RNeasy Mini Kit (Qiagen) according to the manufacturers instructions. Total RNA was used for library construction and sequencing was performed on the Illumina HiSeq 4000 platform.