Long read ONT whole genome sequencing of Rubus idaeus cultivar Varnes
Sequencing libraries were prepared using the SQK-LSK114 Ligation Sequencing Kit (Oxford Nanopore Technologies) from approximately 1 g of high molecular weight genomic DNA, following the manufacturer's protocol. The resultant long-read libraries were sequenced on a single Oxford Nanopore R10 Flow cell with Q20+ chemistry using the PromethION platform (Oxford Nanopore Technologies) set to high accuracy base calling by Novogene (Cambridge UK).