16S of L1 gut in high infested soil
PCR amplification of targeted regions was performed by using specific primers connecting with barcodes. The PCR products of proper size were selected through 2% agarose gel electrophoresis. The same amount of PCR products from each sample was pooled, end-repaired, A-tailed, and further ligated with Illumina adapters. Libraries were sequenced on a paired-end Illumina platform to generate 250bp paired-end raw reads.The library was checked with Qubit and real-time PCR for quantification, while a bioanalyzer was used for size distribution detection. Quantified libraries were pooled and sequenced on Illumina platforms according to the effective library concentration and data amount required.