Bulk RNA-seq of human thalamic and cortical organoids
Total RNA was isolated from 2-3 pooled organoids using the Zymo RNA Microprep Kit. RNA was then Dnase treated and quality was checked by the 4200 TapeStation D1000 ScreenTape Assay (Agilent). Libraries were constructed using Illumina Stranded mRNA prep. Paired end 100 cycle sequencing was performed on a NovaSeq 6000 (Illumina) in the St. Jude Hartwell Center Genome Sequencing Core.