Acropora hyacinthus sample from individual collected at Lady Musgrave reef site
ITS2 rDNA marker genes were amplified from DNA extracts from coral samples, using SYM_VAR_5.8S2 and SYM_VAR_REV primers, adapterma barcodes and illumina adaptors (Hume et al 2019). PCR products were bead purified (PCR_DX), Samples were indexed and sequenced at the Australian Genome Research Facility in Melbourne, Australia. Sequencing was perfomed using an Illumina MiSeq 500 to produce paired-end 2x250bp reads.