target genomics of Phytomyza ilicicola
Extract whole genomic DNA using the DNeasy Blood & Tissue Kit, Sheared DNA using a Covaris S220/E220 Focused-ultrasonicator, library preparation was conducted following the protocol with NEBNext UltraTM II DNA Library Preparation Kit for Illumina NEB7103 with sample purification beads; Dual index primers (Set 1 and Set 2) supplied in the NEBNext Multiplex Oligos Kit were emplyed for Illumina sequencer; Indexed samples were subsequently pooled; target enrichment was performed on each pool using the Diptera AHE probes with SureSelect Target Enrichment System Kits.