Metabarcoding of gypsum endoliths from Israel
DNA extraction using NucleoSpin Soil Kit (Macherey-Nagel) (buffer SL1 and Enhancer SX), amplification and barcoding of samples using primers based on 515f and 926r, containing 12-bp Golay barcodes and 5-bp barcodes, library purification using Pippin Prep (Sage science) and sequencing using Illumina MiSeq