ITS Seqences  of herbaceous rhizosphere soil-associated fungal communities in a 11-year reclaimed mining area located in the coal-rich emalahleni highveld, South Africa
Total genomic DNA was extracted from herbaceous rhizosphere soils. Amplification of the ITS regions was performed using a PCR approach specifically designed for Illumina instruments. The PCR reaction was purified and barcoded amplicons were pooled in equimolar concentration for sequencing on the illumina Miseq platform.