RNA-Seq of Porphyromonas gingivalis W83: Treated with Naringenin replicate 1
Remove the ribosome, and the mRNA would be fragmented into 200nt, cDNA synthesis, End repair, add A and adaptor ligation, Finally, the Qualified libraries will amplify on cBot to generate the cluster on the flowcell, and the amplified flowcell will be sequenced pair end on the Novaseq6000 System (NovaSeq6000 S2 Reagent Kit v1.5,Illumina,USA) with read length 150bp.