RNAseq of LCL C934
Total RNA was isolated by using mirVANA Isolation Kit (ThermoFisher) and the quality assessed by gel-electrophoresis. Directional RNA-Seq libraries were prepared from 150 ng of total RNA using the TruSeq Stranded Total RNA Sample Prep Kit (Illumina) according to the manufacturers protocol. The Illumina RiboZero rRNA Depletion Kit was used to remove rRNA. Qualities of sequencing libraries were assessed with D1000 ScreenTape Assay using the 4200 TapeStation System (Agilent, Santa Clara, CA, USA) and quantified with Qubit dsDNA HS Assay Kit (Invitrogen, Carlsbad, CA, USA). Sequencing was performed on an Illumina NextSeq 500 platform (Illumina, San Diego, CA).