At-C32I-3
Arabidopsis thaliana Col-0 seeds were surface sterilized with ethanol 70% three times for five minutes followed by absolute ethanol for ten minutes, the ethanol was decanted and the seeds were placed in square plates containing 0.2X MS agar medium pH 5.7, supplemented with sucrose [0.5% w/v] and 1% [w/v] agar (Murashige & Skoog, 1962). The plates were placed at 4C for 48 hrs for vernalization and then were incubated at 22  2C in a plant growth chamber with 16 hrs/ 8 hrs light/dark cycles. After four days of germination, 30 l of each bacterial suspension [normalized to O.D. 0.6] were placed on opposite site of the plants. For RNA-seq analysis, rosette leaves of Col-0 plants were inoculated with Acinetobacter C32I. Ten leaves per rosette were collected at 6 hpi and under non-inoculated conditions. Total RNA for RNA-seq was isolated from two different biological replicates for each treatment using the TRizol method according to the manufacturers instructions [Invitrogen]. Total RNA concentration and purity were measured with NanoDrop spectrophotometer [Implen NP80, Thermo Fisher Scientific, USA] Library construction and sequencing were performed by Beijing Genomics Institute [BGI] Americas2 using DNBSeq TM technology