snRNA-seq of mus musculus: 93M adult male mid-hindbrain
The cell suspension was loaded into Chromium microfluidic chips with 3 v3 chemistry and barcoded with a 10 Chromium Controller (10X Genomics). RNA from the barcoded cells was subsequently reverse-transcribed and sequencing libraries constructed with reagents from a Chromium Single Cell 3 v3 reagent kit (10X Genomics) according to the manufacturers instructions. Sequencing was performed with Illumina NovaSeq according to the manufacturers instructions (Illumina).