RNA-seq of adult human gamma9 delta2 T cells
Sequencing libraries were prepared using Illumina platforms. Samples were run on NovaSeq PE150 (instrument identifier number: HWI-ST1276) with a minimum of 20 million single-end reads. Fastq files were mapped and aligned to GrCh38p13 and GenCode36 using Illumina Dragen v3.10.4a on Amazon Web Services. Quantification of aligned samples was achieved using salmon through Illumina Dragen v3.10.4a. Quantification files were imported into R using tximport, low counts were filtered out, and differential expression analysis was performed using DESeq2.