sciMETv2.SL preparation of human middle frontal gyrus using variable adapters sequencing run 5.
sciMETv2.SL preparation of human middle frontal gyrus using the sciMETv2 protocol for combinatorial indexed single-cell DNA methylation profiling. This method involves fixation of isolated nuclei followed by nucleosome disruption to enable genome-wide access during indexed Tn5 tagmentation using methylated adapters. Nuclei are then pooled and distributed to new wells of a plate for bisulfite conversion, reverse adapter incorporation, and indexed PCR amplification.