RNA-seq of S. aureus USA300: low pO2 TSB OD600 0.5
Samples were DNAse treated with Invitrogen DNAse (RNAse free). Library preparation was performed using Illumina's Stranded Total RNA Prep Ligation with Ribo-Zero Plus kit and 10bp unique dual indices (UDI). Sequencing produced 150bp reads. Demultiplexing, quality control, and adapter trimming were performed with bcl-convert (v4.2.4)