Archaeal 16S metagenom of Fe-Mn sediment layer 23-24 cmblf
The V5-V6 variable region of the archaeal 16S rRNA gene was amplified by PCR with  primers,containing rRNA-specific parts: A787F(5-ATTAGATACCCSBGTAGTCC) and A1043R (5-GCCATGCACCWCCTCT). The libraries were analyzed using Illumina MiSeq Standard Kit v.3 (Illumina) at the Core Centrum "Genomic Technologies, Proteomics and Cell Biology" (ARRIAM, Saint-Petersburg).