BS-seq of P. cynomolgi sporozoites: extracted salivary glands from A. dirus
Collected from A. dirus mosquitoes infected from feeding on a rhesus macaque infected with P. cynomolgi M/B strain at ENPRC and cryopreserved. Sporozoites were lysed and purified with phenol-chloroform. Each sample subjected to bisulfite conversion using the Epitect fast bisulfite conversion kit, and libraries prepared using the Accel-NGS methyl-Seq DNA library kit.