16S rRNA seq of facial microbiome
Illumina demultiplexing paired-end sequence reads were generated using the Illumina MiSeq system. The sequences generated with the MiSeq system were performed under accreditation according to the scope of BaseClear B.V. (L457; NEN-EN-ISO/IEC 17025). When paired-end sequencing is being performed, the "Number of reads" noted in this report is referring to read pairs. FASTQ read sequence files were generated using bcl2fastq version 2.20 (Illumina). Initial quality assessment was based on data passing the Illumina Chastity filtering. Subsequently, reads containing PhiX control signal were removed using an inhouse filtering protocol. In addition, reads containing (partial) adapters were clipped (up to a minimum read length of 50 bp). The second quality assessment was based on the remaining reads using the FASTQC quality control tool version 0.11.8. The final quality scores per sample are provided in the Next Generation Sequencing project.