PacBio HiFi WGS of Actias luna: 5th instar larva
SMRT bell libraries were prepared at the University of Florida Interdisciplinary Center for Biotechnology Research (ICBR; RRID:SCR_019152) and sequenced on the PacBio SEQUEL IIe following manufacturer protocol (P/N 101-853-100 v. 05, August 2021) with a few modifications. The library construction steps included: DNA Damage Repair, End Repair/ A-Tailing, SMRT Bell Adaptor ligation, and ExoIII/ExoVII Nuclease treatment. The final libraries (~1.6 g) were size-selected using a SageELFTM instrument (#ELD7510), using 0.75% agarose gel cassettes and the 1-18 kb v2 cassette definition program. The desired SageELF fractions were cleaned using AMPure magnetic beads and eluted in 15 ul of 10 nM Tris HCl, pH 8.0. Library fragment size was estimated by the Agilent TapeStation and these data were used for calculating molar concentrations, resulting in ~650 ng of SMRTbell library fragments of ~11.5 kb size.