Amplicon of mouse stool CPS HFD ZT12_1
DNA was extracted from the fecal samples using QIAamp PowerFecal Pro DNA Kit (QIAGEN, Germany). The V3-V4 region of the 16S rRNA gene was amplified from the fecal DNA samples by PCR using the following primers: forward, 5-TCGTCGGCAGCGTCAGATGTGTATAAGCGACAGCCTACGGGNGGCWGCAG-3; and reverse, 5-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC-3. Library construction was performed in which the flow cell binding sequence and indexed adapters were ligated on the ends of the PCR products using for Nextera XT Index Kit v2 (Illumina, USA).