WGS of Homo sapiens: tRCC
Whole-genome DNA libraries were constructed using KAPA Biosystems (KAPA Hyper Prep with Library Amplification Primer Mix, product KK8504). For cluster amplification and sequencing steps, sample pools were combined with HiSeqX Cluster Amp Regents EPX1, EPX2 and EPX3 into single wells on a strip tube using the Hamilton Starlet Liquid Handling system. We then used Illumina cBot for cluster amplification according to the manufacturer's protocol (Illumina). Flowcells were then sequenced on HiSeqX utilizing sequencing-by-synthesis kits to produce 151bp paired-end reads.