RNA-Seq of Homo sapiens:HeLa-si-NC-1
In general, we utilized RNA extracted using the Trizol reagent (Invitrogen) to construct an rRNA depletion library (Ribo-Zero rRNA Removal Kit, epicentre) following the manufacturers instructions. The RNA sequencing data, generated by the Hiseq Sequencer, underwent filtering and mapping to the human genome 38.