Whole Genome Sequencing of Carassius auratus
Samples were collected, and high molecular weight genomic DNA was prepared by the CTAB method and followed by purification with QIAGEN Genomic kit (Cat#13343, QIAGEN) for regular sequencing, according to the standard operating procedure provided by the manufacturer. After the sample was qualified, size-select of long DNA fragments were performed using the PippinHT system (Sage Science, USA). Next, the ends of DNA fragments were repaired, and A-ligation reaction were conducted with NEBNext Ultra II End Repair/dA-tailing Kit (Cat# E7546). The adapter in the SQK-LSK109 (Oxford Nanopore Technologies, UK) was used for further ligation reaction and DNA liabrary was measured by Qubit 4.0 Fluorometer (Invitrogen, USA). About 700ng DNA library was constructed and performed on a Nanopore PromethION sequencer instrument (Oxford Nanopore Technologies, UK)