small RNA smaples from Arabidopsis thaliana
rep1,Total RNA (30 g) was resolved on 15% urea-PAGE gel, and the 15-30 nt regions were cut from the gel. The sRNAs were recovered by soaking the smashed gel in 0.3 M NaCl overnight, followed by precipitation with ethanol. Then the total sRNAs were used for sRNA libraries construction using the NEBNext Small RNA library Prep Set for Illumina (NEB, E7300S