Pyrus communis 'd'Anjou' genome v2
About 20g of leaves was collected from one 'd'Anjou' plant. High molecular weight DNA was isolated using using a Circulomics Nanobind Plant Nuclei Big DNA kit (Baltimore, MD), with 4 g of input tissue and a 2 hour lysis. DNA was tested for purity via spectrophotometry, quantified by Qubit dsDNA Broad Range, and sized on an Agilent Femto Pulse. DNA was sheared with a Diagenode Megaruptor and size-selected to roughly 25 kb on a BluePippin. A PacBio sequencing library was produced using the SMRTbell Express Template Prep Kit 2.0, and CCS (HiFi) reads were produced on two 8M flow cells.