Mock-infected piglet testicles-1
Total RNA was extracted from the tissues, and only high-quality samples meeting specific criteria were used for library preparation. RNA-seq transcriptome libraries were prepared following the Illumina Stranded mRNA Prep protocol, involving steps such as mRNA isolation, cDNA synthesis, fragmentation, end-repair, and PCR amplification. The paired-end libraries were sequenced using the Illumina NovaSeq Xplus platform. Bioinformatic analyses included quality control and trimming of raw sequencing reads using fastp software, followed by mapping the clean reads to the reference genome using HISAT2. The mapped reads were then assembled into transcripts using StringTie with a reference-based approach.