Gills transcriptome of Serrasalmus rhombeus
RNA-seq libraries have been prepared according to Illumina's protocols using the Illumina TruSeq Stranded mRNA sample prep kit to analyze mRNA. Briefly, mRNA were selected using poly-T beads. Then, RNA were fragmented to generate double stranded cDNA and adaptators were ligated to be sequenced. 11 cycles of PCR were applied to amplify libraries. Library quality was assessed using a Fragment Analyser and libraries were quantified by QPCR using the Kapa Library Quantification Kit. RNA-seq experiments was sequenced in paired end 2*150nt mode on NovaSeq6000 (Illumina), using a lane of a SP flow cell.