16S rDNA of halophilic bacteria
DNA isolation was performed according to Tpper et al. (2010). Bacteria were placed in LB medium and incubated at 28 C for 48 h at 160 rpm. Then, 1 mL of each bacterial suspension was placed in an Eppendorf tube and centrifuged at 14,000 rpm for 3 min. Subsequently, DNA extraction was performed with the commercial DNeasy Blood & Tissue Kit from Qiagen according to the manufacturers instructions. The 16S rDNA gene fragment was amplified by polymerase chain reaction (PCR) with the conditions shown in Table S4. The universal primers were 16SF direct: 5-GCCTAACACATGCAAGTC-3 and 16SR reverse: 5-AAGGAGGTGATCCAGCCGCA-3. A reaction mixture was prepared for each oligonucleotide pair, as described. The PCR product was approximately 1500 bp, which was verified on 1% agarose gel. Subsequently, the 16S rRNA sequences were compared with the GenBank database using the NCBI BLAST nucleotide search