DNA-Seq of Capreolus pygargus: skeleton remain
The ancient Siberian roe deer skeleton remain was labeled with archaeological ID M48(T4) and lab code NJ26S. An electric grinding tool and sterilized disposable drills were used to remove the surface of 2 mm bone samples to prevent external contaminant contamination. Then, the cleaned bone samples were immersed in a 10 % chloramine solution, followed by decontamination with DEPC water and 5 min immersion in 70 % ethanol. Finally, UV-light irradiated the samples until they were dry. The next day, the bone samples were ground into powder using an electric grinding tool, and 200 mg of each sample was collected. DNA extraction of ancient DNA followed the method of Yang et al. (Yang et al., 1998), using the QIAquick PCR Purification Kit kit combined with ultrafiltration tubes (Millipore) to obtain the extraction solution, which was then used to construct a library with the experimental method developed by the Max Planck Institute in Germany (https://www.protocols.io/view/a-z-of-ancient-dna-protocols-for-shotgun-illumina-36wgq529xgk5/v2/guidelines).After the DNA library purified, an Illumina Hiseq X Ten platform was used for whole gene sequencing at Novogene Inc, Beijing, China. Mitochondrial capture was performed on iGeneTech Bioscience Inc, Beijing, China.