RNASeq of Pacific herring liver VEN infection
Tissue samples were collected at days 10 and 34 from two temperatures, with control and VEN infected samples from each timepoint and temperature (n=6). Total RNA wwas extracted using a Direct-zolRNA Miniprep Kit TRI Reagent protocol (Zymo Research). Tag-seq libraries were prepared using the NEBNext Ultra II Direction RNA Library Prep Kit. Final libraries were sequenced as single-end 750 bp reads across two lanes of an Illumina NextSeq500. All libraries were sequenced in both lanes, and files concatenated prior to analysis