Draft genome of Pseudomonas sp. strain CD3
The genomic DNA was extracted from the isolates, grown overnight in Luria broth (Himedia) at 30C and 100rpm. Then DNA library was prepared by using Illumina (Novaseq 6000), 150PE from the genomic DNA and raw data quality was checked using FastQC and MultiQC software. The data was checked for base call quality distribution, % bases above Q20, Q30, %GC, and sequencing adapter contamination. Then the raw sequence reads were processed to remove adapter sequences and low-quality bases using fastp v0.12.4 with default parameters. The QC passed reads were assembled to contigs using Unicycler v0.5.0. Contigs shorter than 200 bp were removed from the assembly. The assembled genome statistics were assessed using QUAST v5.0.2. The assembly quality was checked by mapping the reads back on to the assembled contigs using bowtie2 v2.4.5. The genome completeness was checked using BUSCO v5.3.2 with bacteria_odb10 as reference. The genome completeness and contamination was also checked using CheckM v1.2.1 tool.