Freshwater Hilsa gut metagenome biological replicate 1
The DNA was isolated from the given samples and the DNA concentration was estimated using Qubit Fluorimeter (V.3.0). The V3-V4 region of 16S rRNA was amplified using specific V3 Forward primer CCTACGGGNBGCASCAG and V4 Reverse primer GACTACNVGGGTATCTAATCC. The amplified product was checked on 2% agarose gel and gel purification was done to remove non-specific amplifications.5ng of amplified product was used for library preparation using NEBNext Ultra DNA library preparation kit. The library quantification and quality estimation were done in Agilent 2200 TapeStation. The prepared library was sequenced in Illumina HiSeq 2500 platform.