RNA-seq of Prionotus carolinus: developing bottom pectoral fin before separation
Total RNA was extracted from cells using the Qiagen RNeasy Plus Universal Micro Kit following manufacturers instructions (Qiagen). The SMARTSeq HT Ultra Low Input Kit was used for full-length cDNA synthesis and amplification (Clontech), and Illumina Nextera XT library was used for sequencing library preparation.