Whole genome sequencing of Botia kubotai
Fragmentation of DNA & library generation*****************************************1) Genomic DNA was randomly fragmented by sonication2) DNA fragments were end repaired and A-tailed3) NEBNext adapters containing barcodes were ligated (NEBNext: New England Biolabs)4) PCR amplification5) Purification with AMPure XP system6) Check of library size distribution with Agilent 2100 Bioanalyzer and quantification of library by real-time PCRSequencing**********Illumina NovaSeq 6000, 2 x 150bpTargeted insert size: 350bpSequencing of sample BA_1 was performed in two runs (likely due to not enough data generated in first run). See also raw data stats and FastQC results. The two BA_1 runs were combined for the downstream analyses.FastQC of raw Illumina data***************************Fastqc v.0.11.9 (run with standard parameters)Results of all FastQC runs combined with MultiQC v.1.9