18S rRNA  from arable soil DNA: permanent beds, chopped residues with Nitrogen, plot 3
The PCRs were done with nu-SSU-0817 and nu-SSU-1196 tagged-primers in triplicate, purified, quantified, normalized and pooled. Sequencing was done by Macrogen Inc. with a Roche 454 GS-FLX TitaniumTM pyrosequencer.