DArT-Seq of Tetragonula hockingsi: female worker
DArT-Seq was performed using the DArT-Seq Platform (DArT PL, Canberra, ACT, Australia) according to the manufacturer's protocol (https://www.diversityarrays.com/). Briefly, DNA from whole bees was extracted using a Qiagen DNAeasy Kit. Reduced representation libraries were generated from this DNA at DArT using PstI-MseI restriction enzymes, followed by sequencing on Illumina HiSeq2500.