DNA-seq of Bombyx mori Nucleopolyhedrovirus: Sanya, Hainan isloate
Genomic DNA was fragmented by mechanical interruption (ultrasonic), and then the fragmented DNA was purified, the end was repaired, the 3 'end was added A, and the sequencing joint was connected. Then the purified fragment was sized by agarose gel electrophoresis and amplified by PCR to form a viral genomic DNA sequencing library. The denovo sequencing of the sequencing library was performed by combining Illumina mixed with Nanopore sequencing technology.