Whole genome sequencing of LSDV from Lesotho
100ng of DNA was enzymatically fragmented into 200 bp lengths using Ion shear Plus reagents and followed by adapters and barcodes ligation. Size selection was performed using Pippin Prep and further amplified for 8 cycles. The amplified barcoded library was loaded onto the Ion Chef Instrument for automated template preparation and chip loading using Ion 540 Kit-Chef. Sequencing was performed on an Ion S5 Next generation sequencing system, with 500 flows to generate 200 bp reads.