small RNA-Seq of Paracoccidioides brasiliensis: zinc deficiency rep1
P. brasiliensis Pb18 (ATCC 32069), in the yeast stage, was cultivated and maintained in solid BHI medium at 36 C. For the zinc deprivation treatment, yeast cells were incubated in MMcM medium (McVeigh/Morton medium) supplemented with the zinc chelator DTPA (Diethylenetriaminepentaacetic). For the control, the MMcM medium was supplemented with 0.03 mM ZnSO4. RNA was extracted using the Trizol method. The extracted RNA was used to obtain libraries of RNAs and microRNAs. RNA samples were fractioned before cDNA synthesis, ligation of adapters and sequencing was performed using the Illumina HiSeq 2500 platform.