ChIPseq of Trypanosoma brucei WT PIP5Pase sample
ChIP-seq was performed with T. brucei BFs CN PIP5Pase expressing endogenously HA-tagged RAP1 and exclusively expressing V5-tagged PIP5Pase WT. 3.0x108 cells at mid-log growth were fixed in 1% paraformaldehyde andn processed for ChIP using truChIP Chromatin Shearing Kit. DNA was sheared to a size range of 0.5-1.5 kb . ChIP was performed with a total of 15 g of sonicated chromatin and anti-V5 monoclonal antibody. After Proteinase K incubation of the chromatin, DNA was precipitades and prepared for oxford nanopore sequencing.