WGS of Homo sapiens: tRCC
Whole-genome DNA libraries were constructed using KAPA Biosystems (KAPA Hyper Prep with Library Amplification Primer Mix, product KK8504). ), and with palindromic forked adapters using unique 8-base index sequences embedded within the Roche adapter. Libraries were quantified using quantitative PCR from KAPA Biosystems with probes specific to the ends of the adapters. This assay is automated using Agilent's Bravo liquid handling platform. Based on qPCR quantification, libraries were normalized and pooled into 24-plexes. Sample pools were then combined with NovaSeq Cluster Amp Reagents DPX1, DPX2 and DPX3 and loaded into single lanes of a NovaSeq 6000 S4 flowcell cell using the Hamilton Starlet Liquid Handling system. Cluster amplification and sequencing occur on NovaSeq 6000 Instruments utilizing sequencing-by-synthesis kits to produce 151bp paired-end reads.