RNA-seq of Macrobrachium nipponense
Total RNA was extracted from independent biological replicates and prepared for double-stranded cDNA library construction. The first and second cDNA strands were synthesized successively by using a NEBNext Ultra RNA Library Prep Kit (NEB, Massachusetts, USA). Then, the qualified and amplified mRNA libraries were sequenced on an Illumina NovaSeq 6000 platform, and 150 bp paired-end raw short reads were generated.