Oncorhynchus mykiss gut metagenome: E treatment-tank3-replicateA
Hypervariable region of the bacterial 16S rRNA was amplified by PCR, , using primers S-D-Bact-0341-b-S-17, 5-CCTACGGGNGGCWGCAG-3 and S-D-Bact-0785-a-A-21, 5-GACTACHVGGGTATCTAATCC-3, resulting in an amplicon of 445 pb. Barcode sequences were attached during a second PCR, including a locus sequence adapter suitable for the Illumina sequencing technology. The obtained library was sequenced using the MiSeq Reagent Kit v3 on an Illumina MiSeq platform allowing 600 sequencing cycles to be performed.