Illumina MiSeq sequencing of 5004_S1
Rotavirus dsRNA was extracted using the MagNA Pure Compact RNA Isolation Kit (Roche Applied Science) and served as a template for the cDNA sequencing library using the NEBNext Ultra RNA Library Prep kit and Multiplex Oligos for Illumina kit (New England Biolabs). The library was sequenced using a MiSeq Reagent kit v.2 (500-cycle) on a MiSeq sequencer (Illumina).