HiC data of Bradypodion ventrale
For each Dovetail Omni-C library, chromatin was fixed in place with formaldehyde in the nucleus and then extracted. Fixed chromatin was digested with DNAse I, and chromatin ends were repaired and ligated to a biotinylated bridge adapter followed by proximity ligation of the adapter-containing ends. After proximity ligation, the cross-links were reversed, and the DNA was purified. Purified DNA was treated to remove biotin that was not internal to the ligated fragments. Sequencing libraries were generated using NEBNext Ultra enzymes and Illumina-compatible adapters. Biotin-containing fragments were isolated using streptavidin beads prior to PCR enrichment of each library. The library was sequenced on an Illumina HiSeqX platform to produce a sequence coverage of approximately 30x.