Green Lake Casper Biofilm
Genomic DNA extracted from the 07/25/14 Casper biofilm was also used for shotgun sequencing and metagenomic analysis.   DNA (15 ng) was sheared by ultrasonication (Covaris M220 series, Woburn, MA) following the manufacturers protocol.  A next-generation sequencing library was prepared using the Accel-NGS 2S DNA Library Kit for the Illumina Platform (Swift Biosciences, Ann Arbor, MI).  Libraries were quality checked for size and adapter-dimers using a Bioanalyzer 2100 High Sensitivity DNA chip (Agilent, Santa Clara, CA) then sequenced on a MiSeq 1000 sequencer (Illumina, San Diego, CA, USA) in paired-end mode (2 x 300 bp length).