MicroRNA-sequencing of plasma following an overnight fast
Small RNA was extracted using miRNeasy Serum/Plasma Kit (Qiagen, Velencia, CA, USA). Small RNA (sRNA) libraries for RNA-seq were prepared using NEBNext Multiplex Small RNALibrary Prep Set for Illumina (Set 1 and Set 2). Libraries were size selected using a Blue Pippin and single-end 100 nucleotide sequenced on an Illumina HiSeq2000.