Nanopore_Cleandata
Libraries for Nanopore sequencing was constructed with an insert size of 10 kb. First, large fragments of DNA were recovered by Blue Pippin automatic nucleic acid fragment recovery system, and then repaired. Barcode was added by PCR-free method of EXP-NBD104 kit of Oxford Nanopore Technologies Company. Afterwards, the size of fragments was detected by AATI automatic capillary electrophoresis instrument, and the samples were isomolar mixed. Finally, the SQK-LSK109 connection kit was used to link the adapter, and the 10K library was constructed.