HiC of Whitmania laevis
Hi-C was performed using the following protocol: the leech tissues were fixed in 1% formaldehyde solution. Nuclear chromatin was obtained from the fixed tissue and digested using HindIII. The biotinlabeled DNA fragments were enriched with streptavidin beads (Invitrogen) and were sequenced using the Illumina HiSeq 2000 sequencing platform with both directions of 150 bp reads.