THP-1-replicate2
The gel-purified scDNAs were converted into repaired DNA with 5'-phosphorylated and 3'-dA-tailed ends using the End Prep enzyme mix from Vazyme Biotech Co., Ltd. (Nanjing, China), according to the manufacturers instructions. The repaired DNA was ligated with the pGEM-T Easy vector and then PCR-amplified using the easy primers. The PCR product was used to construct libraries and sequenced on an Illumina NovaSeq 6,000 with 150 bp paired-end reads.