Bulk total RNA sequencing of nasal scrape biopsy
RNA quantity and quality were assessed using TapeStation 4200 High Sensitivity RNA tapes (Agilent), and RNA-seq libraries were prepared from 1 ng of total RNA using SMARTer Stranded Total RNA-seq Kit v2 (Taka Bio). Ribosomal cDNA was depleted after RT-PCR. After quality control of cDNA libraries using TapeStation 4200 High Sensitivity DNA tapes (Agilent), dual indexed libraries were pooled and sequenced on a NextSeq 500 instrument (Illumina), 75 cycles, single-end, to an average sequencing depth of 10M reads. FASTQ files were generated using bcl2fastq (Illumina).