RNA-Seq of PBS buffer injected Apis mellifera fed 20P30L diet, 10 whole bees pooled, cage 2
Clean total RNA samples were diluted with RNase-free water to a final concentration of 326.6  84.7 ng/L. The samples were then used to construct poly-A, stranded libraries containing ~750bp sequences with library-specific adapters with the Next Ultra II RNA for Illumina kit (New England Biolabs, Ipswich, MA). The libraries were PCR-enriched, pooled, and sequenced (150bp paired ends) using the Illumina NovaSeq 6000 platform.