RNA-seq of MOLM13 cells, treated with AC220 and HHT, 8 hour, replicate 2
RNA was extracted with the PureLink RNA Mini Kit, and was subject to quality control using the Agilent 2100 Bioanalyzer for size profiling and the Qubit RNA assay for concentration measurement. mRNA for sequencing was enriched by Poly-A tail capturing, after which the cDNA library was prepared by KAPA mRNA HyperPrep Kit. The library underwent denaturation and dilution to achieve the optimal concentration, and Illumina NovaSeq 6000 was used as the sequencing platform to produce 151bp paired-end reads