RNA-Seq of tomato: WT, rep 1
Total RNA was isolated with an extraction buffer containing 100 mM glycine pH 9.5, 100 mM NaCl, 10 mM EDTA on ice, then purified with phenol:chloroform:isoamyl alcohol (25:24:1, pH 4.3), precipitated with ethanol, and resuspended in nuclease-free water. The samples were treated with DNase I, phenol/chloroform extracted and precipitated with ethanol again. The total RNA was polyA-selected, then stranded sequencing library was prepared following the TruSeq protocol by Novogene.