UCE target enrichment of Discothyrea_MG04_F0173: whole organism
We extracted total DNA non-destructively from body tissue with the QIAamp DNA Micro Kit (Qiagen, Valencia, CA, USA). We sheared 5100 ng of each extract to a fragment length of ~ 400 bp with a Bioruptor UCD-300 sonicator (Diagenode, Denville, NJ, USA). We prepared dual-indexed (Glenn et al. 2019; doi: 10.7717/peerj.7755) genomic libraries with the Kapa Hyper Prep Library Kit (Kapa Biosystems, Wilmington, MA, USA), following the modified protocol described by Faircloth et al. (2015; doi: 10.1111/1755-0998.12328). For UCE enrichment, we used the MYbaits UCE Insect Hymenoptera 2.5K v.2 Ant-Specific kit and followed the MYbaits user manual version 3.02 (Daicel Arbor Biosciences, Ann Arbor, MI, USA). We quantified mass concentration and size distribution throughout lab work with a Qubit 2.0 fluorometer (Thermo Fisher Scientific Inc., Waltham, MA, USA) and 2100  Bioanalyzer High Sensitivity assays (Agilent Technologies, Santa Clara, CA), respectively.