GSM7098836: MDAMB231_cnt2_1; Homo sapiens; miRNA-Seq
Cells were lysed in Qiazol and total RNA was extracted using the Qiagen miRNAeasy kit (Cat.No.: 217084) according to the manufacturer's protocol. The library was prepared using the NEBNext Small RNA Library Prep Set for Illumina (Cat.No. E7330S) and the NEBNext Multiplex Oligos for Illumina (Index Primers Set 2, Cat.No. E7500S) following the manufacturers instructions. For sample clean-up after PCR amplification, the Monarch PCR & DNA Cleanup Kit (NEB, Cat.No.: #T1030) was used. As described in the library preparation kit, Agencourt AMPure XP Beads (Beckman Coulter, Inc. #A63881) were used for size selection.