GSM7849079: gB-specific B cells; Mus musculus; RNA-Seq
The immunized mice were euthanized and the spleens were harvested. Then through tissue grinding and red blood cell lysis, the lymphocytes were extracted from the harvested spleens. Then mouse Pan-B cell isolation magnetic beads were used to isolate pan-B cells from collected cells. After cell washing, the B cells were stained with gB-Alexa Fluro467 and gHgL-FITC. Then stained cells were applied to BD FACSmelody sorter to isolate gB- or gHgL-specific B cells. The two antigen-specific B cell samples were further examined for cell viability and quality for single-B cell sequencing for transcriptome and VDJ analysis. Library construction was performed in following of manufacturer's protocol 10x 5 ' scRNA and VDJ library