GSM6701480: Gut muscularis macrophages sham vs pMCAO 24 h (sham bio rep 3); Mus musculus; RNA-Seq
RNA was harvested using Rneasy micro plus kit (Qiagen). extracted RNA was tested using an RNA 6000 Pico kit on the Agilent 2100 Bioanalyser, 2.0 ng of total RNA was used for the construction of sequencing libraries. An RNA cleavage approach was carried out to remove ribosomal RNA. Library construction and cDNA synthesis were performed using the Trio RNA-sequencing library preparation kit (protocol M01440v2, 2017; Tecan). Manual denaturing and on-board clustering were carried out using 70pM of library pool containing 1% PhiX (protocol 1000000109376 v3, 2020; Illumina). Sequencing was carried out on the NextSeq 2000 (Illumina) with P3 reagents and paired-end reading of 100 base pairs. Approximately 35 million reads were sequenced for each sample