GSM6466263: KD_2; Rattus norvegicus; RNA-Seq
RNA was harvested using TRIzol (15596-018-056, Thermo Fisher, Cleveland, OH, USA) followed by purification using RNeasy Mini Kit (Qiagen, Valencia, CA, USA). 1.3 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.