GSM8012761: control mice, salivary gland, immune cells, GEX; Mus musculus; OTHER
For scRNA-seq, mouse submandibular glands were cut into small pieces and digested with RPMI 1640 (Gibco) containing 1mg/ml collagenase type II (Sigma) in a shaking incubator at 180 rpm at 37°C for 20min. After digestion, PBS containing 2% BSA was added to the submandibular gland cells to terminate digestion. Draining lymph nodes were triturated to separate cells.The isolated cells were filtered through 70-μm nylon mesh and stained with CD45.2 (104, BD Biosciences). CD45+ immune cells were collected by fluorescence-activated cell sorting using BD Aria III (BD Biosciences). Single-cell library preparation was carried out according to the BD Rhapsody™ Single-Cell Whole transcriptome analysis alpha protocol (https://www.bdbiosciences.com/) and ST library were constructed according to the 10x protocol (CG000407_VisiumSpatialGeneExpressionforFFPE_UserGuide_RevA)