GSM8285795: IgG1 [ChIP-seq]; Mus musculus; ChIP-Seq
Cells were fixed with 1% Fomaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using an E220 focused ultrasonicator (Covaris). ChIP samples were prepared using the PIERCE MAGNETIC CHIP KIT30 RXNS KIT 4725 (Thermo Fisher, 26157) according to the manufacturer's protocol.