GSM7676870: 155_87_CTRL; Homo sapiens; RNA-Seq
RNA was extracted using miRNeasy mini kit (Qiagen) from each core according to the manufacturer protocol. RNA concentration and integrity were measured by using NanoDrop ND-2000 and 2200 Tape Station. 20 ng RNA input was used for library preparation by AmpliSeq Transcriptome Human Gene Expression kit. The cDNA was then amplified and barcoded using the Ion Xpress RNA-Seq Barcode 1-16 Kit (Life Technologies 4475485). The Ion Chip Kit v2 BC (Life Technologies 4484270) was then loaded using the Ion Chef System (Life Technologies 4484177) with the Ion PI IC 200 Kit (Life Technologies 4488377). Afterwards, the chips were run on the Ion Proton System for Next-Generation Sequencing (Life Technologies 4476610) using the Ion PI IC 200 Kit.