GSM8322286: bone marrow CLP (Lin-CD127+Flt3+) cells, SclCre;JAK2-V617F 1 (SF1) 1; Mus musculus; RNA-Seq
Total RNA from FACS sorted bone marrow CLP (Lin-CD127+Flt3+) cells were prepared using Picopure RNA isolation kit (ThermoFischer Scientific). The quality and concentration of total RNA was determined on Agilent 2100 Bioanalyzer using the Eukaryote Total RNA Pico Assay. Samples with the RNA Index number (RIN) higher than 7 were used for library preparation. RNA was reverse transcribed, and cDNA was amplified with SMART-Seq v2 or v4 (Takara). Libraries were prepared with Nextera XT (Illumina) according to manufacturer's instructions, as previously described (Rao TN , et al. JAK2-mutant hematopoietic cells display metabolic alterations that can be targeted to treat myeloproliferative neoplasms. Blood. 2019;134(21):1832-1846).