GSM8061344: LuCaP145.1 FOXA2 DiMeLo-seq; Homo sapiens; OTHER
The genomic DNA of LuCaP PDX  and LNCaP+FOXA2 cells was extracted with Quick-DNA Miniprep Plus Kit (Zymo, D4068) and fragmented to average size at 8 kb with g-TUBE™ (Covaris, 520079). The DNA libraries were prepared using Ligation Sequencing Kit (ON SQK-LSK110) per manufacturer's protocol. Sequencing was performed on an Oxford Nanopore GridiON sequencer with R9.4.1 flow cells (FLO-MIN106D) from Oxford Nanopore. Adaptive sampling (AS) method was used for targeted sequencing of regions of interest (ROI).