GSM7986099: scRNAseq_human_small_intestine_Sample2.5; Homo sapiens; RNA-Seq
The intestine was washed, cut into 30 pieces of equal length, and incubated with media supplemented with 5mM EDTA and 10mM DTT at 37°C with 5% CO2. Cells were triturated, strained, washed, and labelled with barcodes unique to each segment using the MULTIseq method. The target population was FACS sorted. 10x libraries were prepared for sequencing using standard 10x genomics protocols adapted for MULTIseq as described in McGinnis et al. Nat Methods 2019. Chromium Single Cell 3'