GSM7986537: Kasumi1, shRUNX1, 92, JMJD1C; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes at room temperature. After quenching and cell lysis, chromatin was sonicated and inbubated with antibody/beads. DNA/protein complexes were then eluted and reverse crosslinked at 65 °C for 6 h. DNA was next purified using phenol/chloroform extraction and ethanol precipitation. ChIP-seq libraries were prepared using End-It DNA End-Repair Kit, Klenow Fragment (3´→ 5´ exo-) and Quick Ligation kit. Libraries were amplified using 16 cycles on the thermocycler and then subjected for size selection using AMPure XP beads.