GSM7586118: para-carcinoma tissue3; Homo sapiens; miRNA-Seq
25 pairs of cancer and peritumoral tissues, and the other 13 cancer tissues alone were pathologically confirmed before being frozeninliquidnitrogenand preserved at -80 ºC. Total RNA degradation and contamination of the frozen samples were monitored on 1% agarose gels. The library was constructed using a Small RNA Sample Pre Kit. Using the special structure of the 3 'and 5' ends of Small RNA (complete phosphate group at the 5 'end and hydroxyl group at the 3' end), taking total RNA as the starting sample, the two ends of Small RNA were directly added to the splice. The cDNA is then synthesized by reverse transcription. After PCR amplification, the target DNA fragments were separated by PAGE gel electrophoresis, and the cDNA library was recovered by cutting the glue Qubit2.0 was used for preliminary quantification, the library was diluted to 1ng/ul, and then Agilent 2100 was used to detect the insert size of the library. After the insert size met the expectation, Q-PCR method was used to accurately quantify the effective concentration of the library