Vaginal microbiome from PID 3043661 at week 4
Samples were transferred to Lysing Matrix E tubes (MP Biomedicals, Burlingame, CA, USA) with RLT lysis buffer (Qiagen, Hilden, Germany) and bead-beated on a TissueLyser (Qiagen). Following manufacturer protocol, the AllPrep DNA/RNA/Protein kit (Qiagen) was used to extract DNA. Profiling of the bacterial microbiota of the collected vaginal swab samples was performed by sequencing the V4 (515F/806R) region of the 16S rRNA gene, as previously described.