RNA-Seq of Pisum sativum: pea genotype CE101 = FP, Non-infested (Control), sampled after 24h
Pea plants were infested with 100 five-day-old P123 or LSR1, enclosed in a perforated plastic bag and maintained in a climatic chamber set at 18  1.5 C, 16 h day/8 h night cycle. The aerial parts of the plants were cut at 3 h, 24 h, and 72 h post-infestation (hpi) and flash frozen by liquid nitrogen. All the aphids on the frozen plants were removed by a paint brush. Non-infested plants were treated in the same manner and served as controls. Plant samples were grounded by motors and pestles with liquid nitrogen. For the preparation of RNAseq samples, total RNAs were extracted using a Macherey-Nagel NucleoSpin RNA Plus Mini kit with DNA removal column. The library preparation and RNA sequencing were realized by the Genewiz Company. Ribosomal RNAs were depleted with the NEB Ultra II DIRECTIONAL RNA library preparation kit and RNA sequencing was performed on an Illumina HiSeq to generate 150-nucleotide-long paired-end reads. The libraries were reverse stranded.