GSM8060592: 031m; Homo sapiens; RNA-Seq
Total RNA was isolated using the AllPrep DNA/RNA/miRNA universal kit (Qiagen, Hilden, Germany). NanoDrop (Thermo Fisher, Waltham, MA) was used to measure RNA concentration. Biopsies were sent for RNA sequencing in case tumor purity was ≥ 30% and RNA concentration was above 20 ng/µL. Library preparation was performed using Total RNAlibrary prep RiboErase (Roche, Basel, Switzerland).