Illumina NovaSeq 6000 paired end sequencing; RIP-seq of lncRNAs interacting with EZH2 in INA-6 multiple myeloma cells
RIP-seq of lncRNAs interacting with EZH2 in INA-6 multiple myeloma cells
RNA immunoprecipitation was conducted utilizing Magna Nuclear RIP kit (Millipore; Billerica, MA, USA; cat. no 17‐10520 ). Immunoprecipitation of EZH2 targets was conducted by incubating the samples with 5 µg of anti-EZH2 (cat. no 17-662, Millipore) and anti-IgG Mouse (cat. no CS200621, Millipore) RNA was purified and cleaned by using RNeasy Micro Kit (Netherlands, Qiagen; cat. no 74004) 100 ng of RNA was used for sample library preparation using TruSeq Stranded Total RNA Gold (Illumina) with non-poly-A selection.