GSM7986124: NSCLC tissue mouse 3, total RNA, miRNA-seq, biological rep 1 [NSCLC_4A]; Mus musculus; miRNA-Seq
Total RNA was extracted using QIAGEN miRNA minikit (REF 1038703) and RNA samples were forwarded to library construction. Libraries were constructed from RNA samples with the QIAseq miRNA sequencing kit (REF 1103677) combined with the QIAseq miRNA NGS 12 Index IL (REF 331592). As an initial step, adapters were ligated to the 3' and 5' ends of miRNAs and subsequently, universal cDNA synthesis with Unique Molecular Indices (UMI) assignment, cDNA cleanup, library amplification and library cleanup were performed.