GSM6738973: A09_PB_1, eHAP FNLS SUGP1 G603N, 2 nM PB, replicate 1; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNAqueousTM-96 Total RNA Isolation Kit (ThermoFisher #AM1920) according to the manufacturer's protocol. Poly(A)-enriched RNA was obtained with the poly(A) RNA Selection Kit V1.5 (Lexogen #157.96) and RNA-seq libraries were generated using the CORALL Total RNA-Seq Library Prep Kit (Lexogen #117.96). The libraries were then sequenced using paired-end 150 bp reads with 60 million reads per sample on Nova Seq (S4).