GSM8244523: not_migrated_R4; Homo sapiens; RNA-Seq
RNA from migrated and non-migrated CD8+ T cells of eight patients with AS was iextracted using the QIAGEN's RNeasy Plus Micro Kit, according to the manufacturer's instructions. Ovation SoLo RNA-seq Library Preparation kit (Tecan Genomics, Redwood city, CA) was used for library preparation following the manufacturer's instructions. After quantification, RNA samples quality was tested by Agilent 2100 Bioanalyzer RNA assay (Agilent technologies, Santa Clara, CA) or Caliper (PerkinElmer, Waltham, MA). Final libraries were checked with both Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA) and Agilent Bioanalyzer DNA assay or Caliper (PerkinElmer, Waltham, MA). The libraries were sequenced on paired-end 150 bp mode on NovaSeq 6000 (Illumina, San Diego, CA).