GSM7677897: DIPG-XIII_Control_B; Homo sapiens; RNA-Seq
RNA was isolated using a Quick-RNA Miniprep kit (Zymo Research, Irvine, CA, USA). RNA quality/quantity of each samples was checked using RNA Nano assay using Bioanalyzer 2100 (Agilent Technologies - RNA RIN should be >8) and the RNA sample quantity was measured  using Qubit 2.0 Fluorometer (Thermo Fisher Scientific). Using 1000 ng of RNA from each sample to start library prep using TruSeq Stranded Total RNA Gold (Illumina RS-122-2303). Sequencing library preparations were performed by Truseq Stranded Total RNA Sample Reference Guide (Illumina). RNA-seq  library quality/quantity checking was done using  Tape Station 2200 D1000 assay (Agilent Technologies) and QuantStudio 6 Flex Real time PCR System (Applied Biosystem). RNA-seq libraries were sequenced in Hiseq 3000 (Illumina) and the sequence type used was Paired End with 75 cycles.