GSM6738770: Hepatic stellate cells, OCA+VIP, 3h, rep A; Mus musculus; RNA-Seq
Medium was removed and cells lyzed in Trizol directly in plates and snap-frozen. After addition of chloroform and separation of phases, RNA was purified from the aquous phase using RNeasy micro columns. Libraries were constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina (according to manufacturer)