Identification of viruses in human saliva
Viral DNA was extracted from 200 l of each sample using the High Pure Viral Nucleic Acid Kit (Roche Diagnostics, USA). Circular DNA in the samples was amplified by rolling circle amplification via the Illustra TempliPhi Kit (GE Healthcare, USA). Illumina sequencing libraries were generated using the Illumina DNA Prep kit and samples were sequenced on the Illumina NovaSeq 6000.