GSM8012944: Photon, organoids, day 11; Mus musculus; RNA-Seq
Organoids were collected and dissocited into single cells using 0.05% trypsin EDTA for 10 minutes. Cells were then washed with PBS/BSA 0.2% and resuspended with 500 µL PBS/BSA 0.2% and propidium iodide (PI). 45.000 PI negative cells/sample were sorted using a Moflo Astrios sorter and immediately leaded into the 10x Genomics chip. For the GEM generation and barcoding, immediately after sorting samples were loaded on the Chromium Next GEM Chip G and ran in the Chromium Controller. The preparation of the library was performed by using the 10X Chromoum Next GEM Single Cell 3' kit T Set A following the manufacturer`s protocol step by step.