GSM7592896: K562_dtagknockin_DMSO_12hr_CTCF_HiChIP_rep1; Homo sapiens; OTHER
Cells were fixed in 1% formaldehyde, washed, snap frozen, and stored at -80ºC. Nuclei were isolated and chromatin was subjected to DPNII digestion, biotin fill-in, and in situ ligation. Chromatin was then sheared with a Covaris sonicator and CTCF-bound chromatin was immunoprecipitated overnight. DNA was tagmented with Tn5 and amplified by PCR.