GSM6403206: Liver cells, Mock, Day1, Biorep3; Mus musculus; RNA-Seq
RNA was harvested using Rneasy mini plus kit (Qiagen). 300 ng of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using  300 ng input total RNA enrichment by poly-dT pull down with NEBNext Poly(A), fragmented to 350 nt, with Ultra II Directional RNA Lib Prep from ILL RNA with NEB adaptor (0.6µM); 12 PCR cycles; FA, following manufacturer's protocols.