GSM7585659: PMDC05, LPS, rep2; Homo sapiens; RNA-Seq
Cells were incubated for 24 hours and harvested for RNA using Takara MiniBest Universal RNA Extraction Kit following manufacturer's instructions. Extracted RNA were stored at -80°C and concentrations were quantified immediately using a NanoDrop spectrophotometer. RNA samples were diluted to obtain a final concentration of 1 - 5 ug/mL to allow for analysis using Bioanalyzer RNA Pico Kit (Agilent). Preparation of RNA samples for analysis using the Bioanalyzer kit was performed according to manufacturer's instructions to acquire appropriate RIN numbers. RNA libraries for RNA-seq were prepared following DNBSEQ Strand-Specific Transcriptome Library Construction Protocol