GSM8285614: iPS_PluriComp_H3K27ac_rep2; Homo sapiens; OTHER
Cells were collected from 10 cm dishes by brief incubation at 37℃ in 0.05M EDTA in HBSS to promote detachment followed by cell scraping. Briefly, cells were attached to Concanavalin-A-conjugated magnetic beads (ConA beads, Polysciences) before overnight binding of primary antibody H3K27ac (Abcam, ab4729). Samples were then incubated with secondary antibody and CUT&Tag-ITAssembled pA-Tn5 Transposomes, after which tagmented DNA was purified This DNA was PCR amplified using a unique combination of i5/i7 indexing primers for each sample, cleaned using SPRI beads and pooled into an equimolar library for sequencing on an Illumina NextSeq 500 sequencing platform.