RNA-seq of persimmon fruit: treated by melatonin and CK
A total of 51 samples (0 d, 1 d, 2 d, 4 d, 6 d, 10 d, 14 d, 20 d, 26 d for CK and melatonin treated fruit) with three biological replicates were used for RNA-seq analysis by Biomarker Technologies Corporation (Beijing, China). The total RNA extraction using the RNAprep Pure Plant Kit (Tiangen, Beijing, China) according to the instructions provided by the manufacturer. Then, the NanoDrop 2000 (Thermo Fisher Scientific, Wilmington, DE) was applied to measure the RNA concentration and purity. RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA). The RNA-seq libraries were prepared by NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) and the libraries were sequenced on the illumina NovaSeq 6000 (Illumina, San Diego, CA, USA). The adaptors were removed and the reads<100 bp were discarded from the raw data to acquire the clean data.