Illumina NovaSeq 6000 paired end sequencing; Transcriptome changes triggered by the loss of function of gene Mppcs in genome-edited Marchantia polymorpha plants
Transcriptome changes triggered by the loss of function of gene Mppcs in genome-edited Marchantia polymorpha plants
About 100mg for each each sample were harvested, snap frozen in liquid nitrogen and stored at -80 °C till use for RNA-seq analyses. M. polymorpha plants were grown in a growth chamber in 1/10 modified Hoagland (Tennstedt et al. 2009, Fischer et al. 2014) solid medium under long-day photoperiod (16h light/8h dark) at 21 °C with a light intensity of 60 µmol m-2 s-1. 100 mg of frozen plant material was used for RNA extraction with the Spectrum Plant Total RNA Kit (Sigma-Aldrich®) according to the manufacturer's instructions. Amplification-grade DNaseI (Sigma-Aldrich®) treatment was carried out. 1.0 µg total RNA was used for library construction. Paired-end libraries were prepared with NEBNext® Ultra RNA Library Prep Kit (NEW ENGLAND BioLab) by integrating Illumina unique dual index adapters (Illumina).