GAPDH sensor sequencing (SI Fig 2h) in the condition 450 ng GAPDH.s1 + 50 ng ADAR1-p150 + 0 nM PPIB siRNA + 100 nM GAPDH siRNA
mRNA was extracted and reverse transcribed. The resulting cDNA was PCR-amplified using locus-specific primers for the sensor or trigger, gel purified, and shipped to Genewiz for Amplicon-EZ sequencing.