GSM6600249: HiPSC-CMs - Hypoxia/Reoxygenation  - H-R-DIAPH1-4; Homo sapiens; RNA-Seq
High quality RNA samples devoid of genomic DNA from shScr and shDIAPH1 silenced HiPSC-CMs was obtained using RNeasy Plus Mini Kit (QIAGEN, cat#74134). cDNA synthesis was performed using qScript™ cDNA SuperMix (Quanta BioSciences, Inc. cat # 95048). Before proceeding to RNAseq, samples were analyzed using Bioanalyzer High Sensitivity RNA Analysis. All samples used had a minimum RNA Integrity number (RIN) value of 9.5 or above. Raw 52-bp paired-end Illumina read data were processed following standard quality control practices to remove low-quality reads and adapter sequence contamination. Remaining high-quality trimmed read pairs were aligned to the human genome (hg19) using STAR 2.7.3a, and read pair counts per gene were summed with the featureCounts function in subread 1.6.3 using the GENCODE 34lift37 annotation release to obtain a gene expression matrix9-11. Technical replicates across two Illumina sequencing batches were summed.