GSM8242353: Macaca mulatta_Spinalcord_treated with mock rep 4 [23697]; Macaca mulatta; RNA-Seq
Total RNA was isolated from freshly cut FFPE tissue sections that contained the selected CNS ROIs (see Digital pathology methods below) using the miRNAeasy FFPE kit, following the manufacturer's protocol Ribosomal RNAs were depleted from 100 ng total FFPE RNA using the QIAseq FastSelect -rRNA MHR kit and following the FastSelect protocol for KAPA RNA HyperPrep kit, with no fragmentation (Qiagen, Valencia, CA).  Sequencing libraries were generated using the Kapa RNA HyperPrep library preparation and technical data sheet KR1350 v3.20, with minor modifications (Roche Sequencing Solutions, Indianapolis, IN).