GSM7770017: WT, Batch 2, rep 1; Mus musculus; RNA-Seq
Whole hippocampi were dissected on ice from female mice aged 3 months and snap-frozen in TRIzol reagent (Invitrogen). Samples were homogenised in TRIzol, extracted using chloroform and 100% ethanol, and then column-purified. Directional polyA-enriched mRNA libraries were prepared and 150bp paired-end reads were obtained by sequencing on the Illumina Novaseq 6000 platform by NovogeneAIT Genomics (Singapore).