V3-V4 16S rRNA of dolphin gut metagenome: Tursiops truncatus gilli
Amplicons of the V3-V4 region of the 16S rRNA gene were sequenced to determine microbiota composition. In short, from the fecal samples, DNA was isolated with QIAamp Fast DNA Stool Mini Kit (Qiagen, Germany), amplified with barcoding, pooled, and subsequently sequenced with the Illumina MiSeq sequencing system according to the manufacturers instructions (Illumina, USA). The Binary Base Call text-based format for storing biological sequence and corresponding quality scores pipeline (BCL2FASTQ, version 1.8.3, Illumina, USA) was used to convert the sequences into a text-based format for storing biological sequence and corresponding quality score (FASTQ) files after quality checking.