GSM7848765: WT activated CD8 T cells, replicate1; Mus musculus; OTHER
Naïve CD8 T cells were isolated from WT or MHC II-/- mice, labeled with CTV, adoptively transferred into 15 irradiated B6 mice respectively, with one million CD8 T cells per mouse. Splenocytes were isolated and CTV- fast-proliferating cells were sorted. All 0.02 million CTV- WT CD8 T cells sorted and 0.15 million out of 1.2 million CTV- MHC II-/- CD8 T cells sorted were subjected to 10x genomics TCR sequencing.After filtering with a 30-micron MACS SmartStrainer (Miltenyi Biotec), single cells were resuspended in PBS (calcium and magnesium free) containing 0.04% weight/volume BSA, and further diluted to 300 cells/µl after cell count (Countess II FL, Life Technologies). Library preparation was performed following the 10x Genomics Single Cell V(D)J Reagent kit v1.1 protocol