GSM7676622: long telomere; Homo sapiens; RNA-Seq
For each sample, cell suspension was prepared using mild digestion to maintain viability of the sample followed by filtration to obtain single cell suspension. More than 6000 cells were utilized from each condition for the single cell sequencing Library preparation for single cell mRNA-Seq analysis was performed according to the Chromium Single Cell 3ʹ Reagent Kit v3 User Guide (Manual Part Number CG000183 Rev A; 10x Genomics). According to the protocol a given 1.6-fold excess of cells was loaded to the 10x controller in order to reach a target number of 7000 cells per sample. Fragment length distribution of generated libraries was monitored using 'Bioanalyzer High Sensitivity DNA Assay' (5067-4626; Agilent Technologies). Quantification of libraries was performed by use of the 'Qubit® dsDNA HS Assay Kit' (Q32854; ThermoFisher Scientific)