miRNA-Seq of Marchantia polymorpha: mature male vegetative thalli
Marchantia polymorpha subsp. ruderalis was used as wild-type plant for performing all experiments. Plants were grown under continuous light at an intensity of 50-60 mol m-2 s-1, with a temperature of 22C, in axenic cultures on half-strength B5 (Gamborg) medium supplemented with 1% (w/v) sucrose. To induce gametangiophore development, thalli were transferred to high-power blue irradiation (470nm) at a level of 30 mol m-2 s-1, deep red irradiation (660nm) at a level of 30 mol m-2 s-1, and far-red irradiation (735-740nm_ at a range of 20-30 mol m-2 s-1. Total RNA for sRNA NGS sequencing was isolated using a method that enables the enrichment of small RNAs (Kruszka et al., 2013). RNA was extracted from following Marchantia organs: antheridiophores, archegoniophores, male and female vegetative thalli, in three biological repetitions.