16S rRNA of soft fillings of borehole core originating from tunnel 1, borehole 12, depth 214 m
The V3 and V4 regions of the bacterial 16S rRNA gene were sequenced using two-step Nextera PCR libraries with the primer pair 341F (5- CCT ACG GGN GGC WGC AG -3) and 802R (5- GAC TAC HVG GGT ATC TAA TCC -3). The Illumina MiSeq platform with a v2 500 cycles kit was utilized for the sequencing of the PCR libraries.