RNA-Seq of U87-MG Trizol extraction: adult male brain cell line
lncRNA: rRNA was removed using a kit, and the RNA after rRNA removal was fragmented to facilitate subsequent cDNA synthesis. First-strand cDNA was synthesized using random primers and reverse transcriptase, followed by double-strand cDNA. The purified double-stranded cDNA was end-repaired, treated with A, and ligated with a sequencing adaptor. cDNA libraries were obtained by PCR amplification.