GSM7592627: WT brains (including cortex and corpus callosum) from 3 mice, combined, scRNA-seq; Mus musculus; RNA-Seq
P21 mouse cortical tissues from WT and the E-mutant containing corpus callosum (3 animals combined per group) were dissociated with papain at 37 degree for 30 min. Cell solution was prepared using debris and dead cell removal kit with MS separation columns. Single cell gene expression library was prepared according to Chromium Single Cell Gene Expression 3v3.1 kit (10x Genomics) by Single Cell Genomic Core (SCG) at the Baylor College of Medicine. Protocol was based on the instruction (single cell 3' V2; 10x Genomics).