GSM6738431: Germinal Center B cell, rep4; Mus musculus; RNA-Seq
RNA was isolated using RNAClean XP Beads (Beckman Coulter, A63987). Near full-length mRNA was reverse transcribed as previously described (Islam et al., 2014) (Trombetta et al., 2014). Multiplexed libraries were prepared for sequencing using a Nextera XT DNA Library Preparation Kit (Illumina). Libraries were sequenced on an Illumina NextSeq 500 at the Rockefeller University Genomics Core.