GSM7585965: MCF10a_HU_Rep1; Homo sapiens; RNA-Seq
RNeasy Mini Kit, Qiagen First, the mRNA is fragmented randomly by adding fragmentation buffer, then the cDNA is synthesized by using mRNA template and random hexamers primer, after which a custom second-strand synthesis buffer (Illumina) , dNTPs, RNase H and DNA polymerase I synthesis. Second, after a series of terminal repair, A ligation and sequencing adaptor ligation, the double-stranded cDNA library is completed through size selection and PCR are added to initiate the secondstrand enrichment