GSM8322262: fCLIP IgG rep1; Homo sapiens; OTHER
Cells were fixed with 0.1% formaldehyde, quenched and lysed. After IP with specific antibodies, RNAs were extracted by ethanol precipitation. RNAs were ligated with 3' adapter. After gel cut, RNAs were ligated with 5' adapter followed by reverse transcription and PCR amplifcation.