GSM7728460: control2; Homo sapiens; RNA-Seq
Total RNA of the peripheral blood mononuclear cells and human iPSCs were extracted with TRIzol reagent (Thermo Fisher Scientific) according to manufacturer instructions. The full-length double-stranded cDNA generated by SMARTer method was captured using TSC1 and TSC2 probe and then the captured double-stranded cDNA was ligated adapter for Nanopore long-read sequencing using the ligation sequencing kit(SQK-LSK109).