GSM7787755: abf1/2/3/4 mutant whole leaf, control, replicate1; Arabidopsis thaliana; RNA-Seq
For whole leaf samples, total RNA was extracted from leaf tissue harvested from 5-6 week old plants using the Spectrum Total RNA Isolation Kit following the manufacturer's protocol. For guard cell samples, total RNA was extracted from isolated leaf epidermal fragments using the Qiagen RNeasy mini kit following the manufacturer's protocol. RNA-Seq libraries were prepared using the TruSeq V2 mRNA-seq library prep kit following the manufacturer's protocol.