GSM6793107: dsrct PDX; Homo sapiens; RNA-Seq
Total RNA was extracted from frozen DSRCT specimen and PDX with an RNeasy mini kit (Qiagen) in accordance with the manufacture's protocol. cDNA libraries were synthesized from 200 ng total RNA with RNA Prep kit with Tagmentation (Illumina), following manufacturers' instructions. Libraries were quantified by Qubit assay (Thermo Fisher Scientific Inc) and quality was assessed by the High Sensitivity kit on the 4200TapeStation (Agilent)