GSM8285628: BasalCnl_24hr_rep3; Homo sapiens; RNA-Seq
Total RNA was isolated using the NucleoSpin RNA Plus kit (Macherey&Nagel). After quantification and quality control using Agilent 2200 TapeStation, total RNA amounts were adjusted. libraries were prepared using the TruSeq Stranded Total RNA kit with Ribo- zero gold rRNA depletion (Illumina). RNA sequencing was carried out on Illumina NextSeq 500 using the High Output Kit v2.5 (150 cycles, Illumina) for 2 × 75 bp paired-end reads