Illumina HiSeq 4000 paired end sequencing; RNA-Sequencing of whole blood samples from children with a range of acute febrile illnesses
RNA-Sequencing of whole blood samples from children with a range of acute febrile illnesses
Whole blood was collected at the time of recruitment in PAXgene tubes RNA samples were stored at −80 °C until further analysis. After additional DNAse treatment, library preparation at paired end reads was conducted using the Illumina's TruSeq® RNA Sample Preparation Kit; ribosomal and globin RNA depletion