GSM6402434: PDS5B-dTAG_dTAG_PDS5B; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, Chromatin was sheared to an average length of 300-700 bp with a Qsonica sonicator. ChIP-seq libraries were prepared using VAHTSTM Universal DNA Library Prep Kit for Illumina. After amplification and size selection with Agencourt AMPure XP beads from Beckman Coulter, the size distributions were determined with Agilent 2100 Bioanalyzer.