GSM7728467: KO_SMARTA_rep3_ATACseq; Mus musculus; ATAC-seq
The sorted cell pellets were lysed in the lysis buffer (ATAC-seq Kit, Activemotif) and purified with DNA Clean and Concentrator-5 Kit (Zymo Research). Library amplification was performed with NEBNext Ultra II Q5 2X master mix. The ATAC-seq libraries were purified with SPRI Beads (ATAC-seq Kit), quantified with NEBNext Library Quant Kit, and sequenced on the NovaSeq 6000 sequencing system in paired end 150 bp read, with at least 50 million reads per sample.