CapTrap-Seq: A platform-agnostic and quantitative approach for high-fidelity full-length RNA transcript sequencing
CapTrap-Seq: A platform-agnostic and quantitative approach for high-fidelity full-length RNA transcript sequencing
commercial samples obtained from Ambion: Ambion is a brand of life science research products and technologies, mainly focused on RNA products. It is owned by Thermo Fisher Scientific, a company that provides a variety of scientific research products and services. Therefore, \"total RNA commercial samples obtained from Ambion\" refers to RNA samples purchased from this brand for research purposes. Teloprime cDNA library preparation: The Teloprime cDNA library preparation method uses the Poly(A) plus Cap-Dependent Linker Ligation (CDLL) protocol. This involves the selection of mRNA with a poly(A) tail, followed by the addition of a cap-dependent linker to the 5' end of the RNA molecule. The RNA is then reverse-transcribed into cDNA, which is subsequently amplified and used for downstream applications such as next-generation sequencing. This method allows for the detection of full-length transcripts and provides an accurate representation of the transcriptome.