WT retina H3K4me3 C&T, P14
Briefly, cells were thawed on ice and 100,000 bound to ConA beads for each sample. Primary antibody incubation was left at 4C overnight on a nutator. 0.5ug of antibody was used for each reaction; H3K4me3 (MABE647; EMD Millipore Corporation, USA) and H3K27Ac (ab177178; Abcam Inc, USA). 0.5ug secondary antibody (CUTANA anti-rabbit secondary antibody; 13-0047; Epicypher) was incubated for 0.5hr at RT, and Tn5 binding and trnasposition done exactly as specified in CUTANA protocol. After transposition, custom 10bp unique-dual-indexed primers were used to amplify transposed fragments (P7-CAAGCAGAAGACGGCATACGAGAT-10bpBC-GTCTCGTGGGCTCGGAGATGTG, P5-AATGATACGGCGACCACCGAGATCTACAC-10bpBC-TCGTCGGCAGCGTCAGATGTGTAT) for 20 cycles. Libraries were cleaned and size-selected using Ampure XP beads. Libraries were pooled and sequenced using the Illumina NovaSeq 6000.