GSM7588905: H9, MSL3KD RNA-seq Rep4; Homo sapiens; RNA-Seq
RNA was isolated from RNeasy Plus Mini Kit (Qiagen, Cat. No. / ID: 74134 ), we spiked in equal amounts of The External RNA Controls Consortium (ERCC) Spike-in RNA Variant Control Sets (SIRV set 3, Lexogen) and 500 ng of RNA was used for depletion of rRNA using RiboCOP kit (Lexogen). RNAseq library preparation using CORALL Total RNA-Seq Library Prep Kit (Lexogen). Libraries were sequenced as 150 bp paired-end reads using Novaseq 6000.