GSM8013149: ChIPseq for ADNP2 after SETDB1 depletion in wt mES cells, replicate 2; Mus musculus; ChIP-Seq
10-20^6 mES cells were crosslinked, chromatin was extracted and sonicated to an average size of 300bp. 5% of chromatin was set aside as Input control. The chromatin was incubated with antibody and protein A/G coupled Dynabeads, washed and de-crosslinked. Purified DNA was subsequently used for library preparation. For a detailed protocol, see Ahel,Pandey et al. 2024. Libraries were prepared using the NEB Next Ultra II DNA library prep kit according to manufacturers instructions.