RAD-seq of Crataegus pinnatifida: leaf
DNA was extracted by DNeasy plant mini prep kit (Qiagen), and then DNA was digested with EcoRI. P1 adaptor was added at both ends of the enzyme section and DNA of 300bp-500bp was recovered after random interruption by ultrasonic. Add a tail following flattening the end of the sequence, and then P2 adaptor was added to selectively amplify fragments containing P1 and P2 adaptors. Finally, Illumina HiSeq 2500 was used for double-terminal sequencing.