GSM6402517: UMUC9-Chipseq_H3K27me3; Homo sapiens; ChIP-Seq
Cells were fixed with freshly prepared 1% formaldehyde for 10 minutes, quenched with 0.125Mglycine for 10 minutes at room temperature. Next, chromatin was sonicated using E220 focused ultra-sonicator (Covaris). ChIP-seq libraries were prepared with 10ng DNA as input using KAPA HTP Library preparation kit complemented with NEXTFLex DNA barcode from Bioo Scientific and validated using Agilent High Sensitivity DNA kit.