FCAR01_vs_FCAS02
Enrichment of eukaryotic mRNA by magnetic beads with Oligo (dT). Subsequently, fragment buffer was added to randomly interrupt mRNA. Using mRNA as template, the first strand of cDNA was synthesized with six base random primers, then the second strand of cDNA was synthesized by adding buffer, dNTPs and DNA polymerase I, and then the double stranded cDNA was purified with AMPure XP beads.