GSM7099047: C57 mouse lung tissue, Experimental group 1; Mus musculus; RNA-Seq
Exosomes from three sham group and three CLP groups were used to perform RNA sequencing. MiRNeasy Serum/Plasma Advanced Kit (Qiagen, cat. No. 217204) was used for the extraction and purification of total RNA from lung tissue exosomes according to the instructions of the kit. According to the manufacturer's recommendations, SMARTer Stranded total RNA-Seq Kit V2 (Takara Bio USA, Inc.) was used for the construction of sequencing library with 4.5 ng RNA of each sample as the input material, and the index codes was then add to the attribute sequences of each sample.