RNA-seq of Homo sapiens: embryonic bodies
SCA1-iPSCs and normal iPSCs were collected and homogenized in 350 l RNA RLT buffer (Qiagen)/0.01% 2-mercaptoethanol (Wako, Tokyo, Japan). Total RNA was purified with RNeasy mini kit (Qiagen). To eliminate genomic DNA contamination, on-column DNA digestion was conducted for each sample with DNase I (Qiagen).