RNA-Seq of Pinus taeda: Whole Seedling
Two independent RNA extractions were completed for each biological replicate, and the resulting total RNA was pooled into one tube representing a single biological replicate. RNA extractions were done using a 96-well plate protocol (non-straind specific polyA selected) modified from published methods for high-throughput DNA extraction. Library creation, multiplexing, and Illumina HighSeq 2500 single-end sequencing were carried out by the Genomic Sciences Laboratory (GSL) at North Carolina State University in Raleigh, NC, USA