High throughput targeted RNA sequencing on HepG2 cell line exposed to multiple concentration levels of drug induced liver injury (DILI) compounds at various different time points_part2
High throughput targeted RNA sequencing on HepG2 cell line exposed to multiple concentration levels of drug induced liver injury (DILI) compounds at various different time points_part2
Wash the cells with PBS 1 times. Add the 1x BioSpyder lysis buffer. Incubate at the room temperature for 15 mins. Store the lysates at -80'C until sequencing. Nucleic acid extraction was done following the Tempo-seq protocol detailed here: https://www.frontiersin.org/articles/10.3389/fgene.2018.00485/full#h3 Nucleic acid library construction was done following the Tempo-seq protocol detailed here: https://www.frontiersin.org/articles/10.3389/fgene.2018.00485/full#h3