GSM8102088: Y8_γδT17 IL-7 IL-1b IL-23 DMSO; Mus musculus; RNA-Seq
total RNA was extracted using Rneasy plus micro kit (Qiagen). 100 ng of total RNA (RIN>9) was used for library preparation RNA quality was assessed on a Bioanalyzer 2100 using a RNA 6000 Nano chip (both from Agilent, Santa Clara, CA). 100 ng of total RNA (integrity > 9) was used to generate barcoded mRNA-seq cDNA libraries using the NEBNext® Poly(A) mRNA Magnetic Isolation Module and NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (both from NEB, Ipswich, MA) with a final amplification of 13 PCR cycles. RNA was quantified using the Qubit HS assay kit (Thermo Fisher) and the library size distribution was determined using the Bioanalyzer HS DNA assay (Agilent). Those premade libraries were sent to Novogene (Cambridge, UK) and were sequenced on a Illumina NovaSeq 6000 instrument aiming for a minimum of 50 million PE150 reads per sample.