GSM8354427: HCC patient 86; Homo sapiens; RNA-Seq
Genomic DNA and RNA were extracted from 5 μm FFPE tissue sections using the AllPrep DNA/RNA FFPE kit (Qiagen, Valencia, CA, USA). The concentration and integrity of purified DNA/RNA samples were assessed by Qubit fluorometer (Thermo Fisher Scientific, Hampton, NH, USA) and 2200 TapeStatio (Agilent Technologies, Santa Clara, CA, USA), respectively. RNA-Sequencing library were prepared using AmpliSeq for Illumina RNA WNT panel (the panel include 169 genes involved in the regulation of WNT signaling pathway) by following the manufacturer's. Pooled sequencing libraries were sequencing in 150-bp paired-end reads on Illumina MiSeq sequencer (Illumina).