GSM7592891: HCT116_HaloTag_CTCF_minus_HaloPROTAC3_minus_DNMT1i_SON_CutTag_rep1; Homo sapiens; OTHER
Cells were harvested and nuclei were isolated and lightly fixed with 0.1% formaldehyde for 2 minutes. Nuclei were bound to Concavalin A beads and anti-SON antibody was added at 4C overnight. Following addition of secondary antibody and subsequent washes, pA-Tn5 was added. Chromatin was tagmented with PA-Tn5 and DNA was extracted and amplified by PCR.