FigWasp_pool_07
Sequencing libraries were prepared with a Nextera XT Kit (Illumina, San Diego, CA, USA) using 1 ng of total DNA (combination DNA extract and cDNA generated from RNA extract) as input. Library fragment length and quality were evaluated with a Bioanalyzer 2100 using the High Sensitivity DNA Kit (Agilent Technologies, Santa Clara, CA, USA). Libraries were normalized and pooled by following manufacturers protocol and sequenced on the MiSeq platform using MiSeq Reagent Kit v3 (2 x 300 cycles) (Illumina). Selected samples were also processed for additional sequencing with the NEB Next Ultra Directional RNA library kit (New England Biolabs, Ipswich, MA, USA) using the human recombinant RNA (rRNA) subtraction module following a previously published protocol with 1% PhiX spiked in.