GSM8146975: WT, exponential, repl 3; Enterococcus faecalis; RNA-Seq
Total RNA was extracted using the UltraClean® Microbial RNA Isolation Kit (MO BIO Laboratories Inc., Singapore). Extracted RNA samples were subjected to rigorous DNase treatment using TURBO DNA-free™ kit (Ambion®, Singapore) and purified DNA-free RNA samples were subjected to ribosomal depletion with Ribo-Zero™ Magnetic Kits (Epicentre®, Singapore), all according to manufacturer's protocols. Quantification of RNA and DNA were performed using Qubit™ RNA Assay Kits and Qubit™ dsDNA HS Assay Kits (Invitrogen, Singapore), respectively. The integrity of RNA was analyzed by gel electrophoresis using Agilent RNA ScreenTape (Agilent Technologies, Singapore). mRNA libraries for RNA sequencing were prepared using TruSeq Stranded mRNA Library Prep Kit (Illumina, USA), the quality of the library analyzed via Bioanalyzer (Agilent, USA).