Sanger sequencing of Saccharomyces cerevisiae:  Cas3-APOBEC1 targeted to SEC14 locus
A 1839 bp amplicon ranging from 171 bp upstream to 597 bp downstream of the SEC14 (Chr. XIII 424989-426059) start codon was generated via PCR. Amplicons were amplified from 18 colonies able to grow on 3 M NPPM 481-containing medium after induction of the CoMuTER system targeted to a site 386 bp downstream of the SEC14 stop codon. Amplicons were sent for Sanger sequencing at Eurofins Genomics and sequenced using two primers per amplicon (Primer_a: GTTCCGTACAACGCGACTGG and Primer_b: TCAACCGTGAAATGAGCGCTG).