GSM7849394: SINTH8 Hashtag Capture; Homo sapiens; RNA-Seq
Two replicate of IMR90 growing expressing vector control and RIS IMR90 cells expressing shA1 or vector control were individually labelled with 1ug of Biolegend TotalSeq™-B Cell Hashing antibodies diluted in Cell Staining Buffer (PBS with 3%FBS) for 30 minutes at 4˚C, then washed 3 times with Cell Staining buffer (centrifuge 2 minutes 350g at 4˚C). Cells were resuspended to a concentration of 800 cells/uL for single-cell encapsulation using the Chromium Single Cell B Chip Kit (10x Genomics Cat # PN-1000073), followed by library prep using the Chromium Single Cell 3' GEM Library & Gel Bead Kit v3 (10x Genomics Cat # PN-1000075) for the gene expression library and the Chromium Single Cell 3' Feature Barcode Library Kit (10x Genomics Cat # PN-1000079) for the hashtag-oligo library. Both libraries were then pooled for paired-end sequencing on the NovaSeq 6000 platform (Illumina).