GSM8285676: HEK293T-TFE3_TFE3; Homo sapiens; OTHER
Cut&Tag libraries were prepared using the Illumina Nextera adaptors provided by the Active Motif Cut&Tag kit following the manufacturer guidelines. Cells were plated in 10 cm petri-dish for 48h and harvested using CellStripper solution and washed 2 times in PBS 1X. Cells were counted using Trypan blue and the Countess® II Automated Cell counter (Invitrogen). 500000 cells were used for each native Cut&Tag performed using the Active motif Cut&Tag kit (ref. 53160) following the manufacturer provided protocol.  