Amplicon sequencing of human CAR T cells
gDNA was isolated from T cells (NucleoSpin Tissue, Machery Nagel). PCR amplification of the samples was performed using primers to enrich for the mutation barcodes and to add Illumina adapters with Kapa HiFi. Finally, PCR products were quantified using Qubit, and were pooled at a 1:1 molar ratio and run on a MiniSeq 75 cycle cartridge using the standard manufacturer protocols.