GSM7058349: 2102EP MPHv2 SAMv2, cisplatin; Homo sapiens; OTHER
Genomic DNA was isolated using the Quick DNA Midi Prep Plus Kit (Zymo Research, Irvine, CA, USA) Library construction was performed according to Joung et al. 2017 (doi:10.1038/nprot.2017.016). In brief, sgRNA encoding DNA regions were amplified with specific Illumina adapter/bar code labeled primers using NEBNext® High-Fidelity 2X PCR Master Mix (New England Biolabs GmbH, Frankfurt am Main, Germany). DNA sequencing was performed by the Core Facility Next Generation Sequencing (University of Bonn, Germany).