GSM8105769: M17 cells, LINC00894 knockdown, B-3; Homo sapiens; RNA-Seq
Total RNA was extracted by TRIzol(Takara) After the sample was qualified, eukaryotic mRNA was enriched with magnetic beads with Oligo (dT) connections. mRNA was then randomly interrupted by fragmentation buffer. Using mRNA as template, the first strand of cDNA was synthesized with six-base random primers, then the second strand of cDNA was synthesized by adding buffer, dNTPs and DNA polymerase I, and then the double-stranded cDNA was purified by AMPure XP beads. The purified double-stranded cDNA was then end-repaired, A-tailed and sequenced, and then treated with AMPure XP beads were used for fragment size selection and PCR enrichment to obtain the final cDNA library.