GSM7986108: GTL1 pbmc，at Pre-grugery,in no transfusion group; Homo sapiens; RNA-Seq
Peripheral blood mononuclear cells were isolated by density gradient centrifugation using Ficoll Hypaque density solution. Cells were used only if >80% were viable based on trypan blue staining, in which case they were subjected to single-cell RNA sequencing. An appropriate volume of peripheral blood mononuclear cells was loaded onto a 10X Genomics Chromium instrument to capture 8000 single cells according to the manufacturer's instructions for the 10X Genomics Chromium Single-Cell 5' kit (version 2). Subsequent cDNA amplification and library construction were performed according to the standard protocol. Libraries were sequenced on an Illumina NovaSeq 6000 sequencing system (paired-end multiplexing run, 150 bp) at LC-Bio Technology (Hangzhou, China) at a minimum depth of 20,000 reads per cell.