GSM6523359: dSLAM-seq MCMV 72hpi A; Mus musculus; OTHER
Cells were lyzed using Trizol and total cellular RNA was isolated. RNA was subject to alkylation using Iodoacetamide to mediate U to C conversions to distinguish newly synthesized RNA followed by RNA purification following the SLAM-seq protocol (Herzog et al., Nature commun. 2017). Sequencing libraries were prepared using the dRNA-seq library preparation as described in Whisnant A.W. et al, Nat. Commun (2020)