GSM8061539: Control brain, 15-month-old male biol rep #3; Mus musculus; RNA-Seq
Brain tissue was dissected from animals following perfusion with PBS and heparin. Samples were flash-frozen in liquid nitrogen and stored at -80°C. Samples were later placed in a deep-well 96-well plate with stainless steel RNase-free homogenization beads and QIAzol lysis buffer. Plates were inserted into the SPEX SamplePrep 1600 MiniG tissue homogenizer and run at maximum speed for 5 minutes. Following homogenization, the tissue solution was added to QIAzol lysis buffer and stored at -80°C. Lysed tissue solution was subsequently added to the QIAGEN miRNeasy Micro Kit for purification of total RNA. RNA libraries for RNA-seq were prepared using the Kapa RNA HyperPrep kit (Roche) following the manufacturer's instructions.