GSM7187772: fy-5 KO 2; Homo sapiens; RNA-Seq
RNA was extracted using the RNeasy MinElute Cleanup Kit (Qiagen) according to the manufacterers protocol mRNA was treated using the NEBNext rRNA depletion Kit (human, mouse, rat) from New England Biolabs (NEB) according to the manufacturer's instructions to remove ribosomal RNA. Subsequently, the samples were subjected to the workflow for strand specific RNA-Seq library preparation (NEBNext Ultra II Directional RNA Library Prep, NEB).