GSM7057510: leaves, sprayed with 200 mM IAA, 18 h, rep2 (miRNA-seq); Rosa hybrid cultivar; miRNA-Seq
RNA was harvested using Rneasy mini plus kit (Qiagen). 1.3 ug of total RNA was used for the construction of sequencing libraries. The Small RNA Sample Pre Kit was used to construct the library, using the special structure of the 3' and 5' ends of Small RNA (complete phosphate group at the 5' end and hydroxyl group at the 3' end), and using total RNA as the starting sample. After PCR amplification, the target DNA fragments were separated by PAGE gel electrophoresis, and the cDNA library was obtained by cutting and recycling.