GSM7730171: Pharmacoresistant epilepsy, Cortex, right frontal lobe, snRNA-seq; Homo sapiens; RNA-Seq
snRNA-seq was performed on fresh frozen brain tissue from the patient's second epileptic focus resection. 128 mg of frozen brain tissue was cut at –20 °C and sent on dry ice to Singulomics (New York) for nuclei extraction and snRNA-seq using the 10x Genomics v3 platform, targeting recovery of 10,000 nuclei and 25,000 reads per cell.~200 million PE150 reads were sequenced on an Illumina NovaSeq 6000 sequencer. The library (10x v3.1 GEM) was constructed according to standard operating procedure at Singulomics