GSM7984668: BAT, WT, 30C, replicate2; Mus musculus; RNA-Seq
Total RNA was isolated from brown adipose tissue using Direct-zol RNA Miniprep Plus Kit (Zymo Research). Polyadenylated mRNA was purified from 500 ng of total RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB). Sequencing libraries were prepared using NEBNext Ultra II RNA LibraryPrep Kit for Illumina (NEB) according to manufacturer's instructions. Library concentrations were quantified by a Bioanalyzer (Agilent).