Double digest Genotyping by Sequencing (GBS) library of Cucurbita radicans from Mexico
DNA was extracted from fruit peduncle using CTAB. DNA was sequenced by University of Minnesota Genomics Center. Two restriction enzymes (NspI and BfuCI/Sau3AI) were used. Genotyping by Sequencing (GBS) method was performed with an Illumina Novaseq instrument.