GSM8322518: Primary NRCMs, lenti-TCF7L2 shRNA, replicate 3; Rattus norvegicus; RNA-Seq
Total RNA was extracted from lenti-TCF7L2-, lenti-shTCF7L2 -or lenti-NC-infected NRCMs using a Trizol reagent kit. The RNA quality was evaluated on an Agilent 2100 Bioanalyzer (Agilent Technologies, USA) and confirmed with agarose gel electrophoresis. The mRNA was enriched with Oligo (dT) beads, fragmented, and reversely transcribed into cDNA using a NEBNext Ultra RNA Library Prep Kit The double-stranded cDNA fragments were purified and used in a standard Illumina library preparation involving end-repair, A-tailing, adapter-ligation, and amplification by PCR. The resulting cDNA library was subjected to sequencing using the BGISEQ-500 sequencing platform at BGI Genomics (China).