16S-Seq of lemon farmland soil: Greenhouse at the flowering stage
. For investigated the protistan communities, the V9 region of eukaryotic 18S rRNA gene were amplified from the microbial DNA using primers of 1380F (TCCCTGCCHTTTGTACACAC) and 1510R (CCTTCYGCAGGTTCACCTAC) (Stoeck et al., 2009). The detail processes of PCR and library construction referred to previous studies (Zhao et al., 2022). Finally, the libraries were sequenced using the Illumina NovaSeq 6000 platform at BIOZERN Biotech. Co., Ltd (Shanghai, China).