GSM6600088: Δpfk2_b_2D_2; Thermothelomyces thermophilus ATCC 42464; RNA-Seq
Mycelia for RNA isolation were harvested at exponential growth phase (2 days and 4 days). After filtration, the collected mycelia were washed twice and immediately frozen in liquid nitrogen. The samples stored at -80 °C until RNA extraction. About 100mg frozen samples were quickly ground into powder in liquid nitrogen in the mortar. Total RNA was extracted from the powder using RNeasy mini kit (Qiagen) according to the manufacturer's instruction. RNA libraries were prepared for sequencing using standard Illumina protocols