16S amplicon sequencing of bioreactor biome
Genomic DNA was extracted using the DNeasy Isolation PowerSoil Kit (Qiagen, Germany) according to manufacturers instructions. The hypervariable regions V3-V4 of the bacterial 16S rRNA gene were amplified with the primer pair 338F (5'-ACTCCTACGGGAGGCAGCAG-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3'). The PCR product was extracted from 2% agarose gel, purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) and quantified using Quantus Fluorometer (Promega, USA).