GSM8243852: FLX1, PKAi, rep2 [C2]; Homo sapiens; RNA-Seq
RNA samples were extracted using the RNeasy Plus Mini Kit (Qiagen) and subsequently stored at -80°C. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library.