GSM7986300: GFP_Ctrl_rep2; Homo sapiens; RNA-Seq
Total RNA of GFP or NRF2 expressing Hs 578T cells was purified using EuroGOLD TriFast™ (Euroclone, Milan, Italy). RNA quantity and quality were evaluated by Nanodrop 8000 (Thermo Fisher Scientific, Waltham, MA, USA) and Fragment Analyzer (Advanced Analytical Technologies, Heidelberg, Germany) using the DNF-471 Standard Sensitivity RNA Analysis Kit (15nt) (Agilent Technologies, Santa Clara, CA, USA). RNA samples were further processed for mRNA-seq library preparation according to the manufacturer's instructions (TruSeq RNA Sample preparation v2, Illumina, San Diego, CA, USA).