GSM7426246: ECs, 92; Mus musculus; RNA-Seq
Sciatic nerves were freshly harvested in F12medium, pooled, finely chopped and incubated in dissociation buffer (Trypsin 0.2mg/ml, Collegenase Type II 50ul/ml, Hyaluyronidase 1:50, PronaseE 1:10, DNAse 2.5μg/ml) in DMEM and incubated on rotor at 37 C with intermittent mixing for 30 min. Enzymes were inactivated with 5% FBS+ 2mM EDTA in PBS w/o Ca2+, Mg2+. Cell suspension was passed through cell strainer, stained (CD45-APC) and resuspended in 2% BSA PBS w/o Ca2+, Mg2+. Tdtomato+ or Cherry+ (negative for CD45+ myeloid population) were purified by FACS.