Small RNA-seq of Rattus norvegicus: 1 year F3 sperm
Total RNA was used to construct small RNA libraries using the NEBNext Small RNA library kit (NEB, E7330), according to the manufacturers instructions. Size selection was performed on the Pippin Prep (Sage Science) (115-160bp). Quality control was performed using Agilent DNA High Sensitivity chips (Agilent) and Qubit dsDNA high sensitivity assay (ThermoFisher). Libraries were pooled and sequenced on an Illumina HiSeq 2500 sequencer with 1x50 read length.