GSM8321941: kidneys, L3T immunized sample1; Danio rerio; RNA-Seq
After 12 days of the secondary immunization, kidneys of adult zebrafish were isolated and placed in 1mL Trizol for RNA extraction. Fifteen kidneys per group were collected and pooled as three samples.Total RNA was extracted using the TRIzol reagent (Invitrogen, CA, USA) according to themanufacturer's protocol. RNA purity and quantification were evaluated using the NanoDrop 2000spectrophotometer (Thermo Scientific, USA). RNA integrity was assessed using the Agilent 2100Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Then the libraries were constructed usingVAHTS Universal V6 RNA-seq Library Prep Kit according to the manufacturer's instructions.