ATACseq of homo sapiens: B cells
50000 CD19+ B cells were centrifuged for 5 minutes at 500g, resuspended in 50 ul of cold Lysis Buffer (10nM Tris-Cl, 10mM NaCl, 3mM MgCl2, 0.1% IPEGAL CA-630), and immediately centrifuged for 10 minutes at 500g. Tagmentation was performed by resuspending cells in 25 ul of transposase reaction mix (2x TD Buffer, 2.5 ul Tn5 Transposase and 22.5ul nuclease-free water) and incubating for 30 minutes at 37C. DNA was purified and amplified into a sequencing library with SyBR Green and NEBnext PCR kit primers (New England Biolabs).