Illumina HiSeq 2500 paired end sequencing; Mosaic BRAF fusions are a recurrent cause of congenital melanocytic naevi targetable by MEK inhibition
Mosaic BRAF fusions are a recurrent cause of congenital melanocytic naevi targetable by MEK inhibition
Skin biopsies from patients with CMN were obtained either during routine surgery or by a single 4-mm punch skin biopsy. Written informed consent was obtained from their parents/guardians under the local NHS Research Ethics Committee. Total RNA was extracted from CMN tissue using the RNeasy Fibrous Tissue Mini Kit (Qiagen 74704) according to the manufacturerâ€™s protocol. RNA integrity was assessed using a Bioanalyser (Agilent). Libraries were prepared with KAPA mRNA HyperPrep Kit with RiboErase (Roche) using 80ng of total RNA