Tiled RT-PCR of SARS-CoV-2 spike gene from wastewater.
Total nucleic acid from wastewater solids treated with TurboDNase, concentrated, depleted of bacterial rRNA with NEB kit, reverse transcribed with SuperScript IV VILO, amplified with ARTIC v4.1 spike primers using NEB Q5, converted into Illumina-compatible libraries using NEB Ultra II DNA kit, and sequenced on an Illumina system 250PE or 300PE.