GSM7987332: Y79,Scramble,RNA-seq,rep3; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol™ Reagent (Invitrogen, 15596026). Take 1 µg of total RNA and perform a reverse transcription experiment using HiScript III RT SuperMix for qPCR (Vazyme, R323-01) to obtain cDNA. RNA libraries for RNA-seg were prepared using SMARTER mRNA-seq library prep kit following manufacturer's protocols.  The Hyperactive Universal CUT&Tag Assay Kit for Illumina (Vazyme Biotech, TD903) was used for CUT&Tag analysis.