RNA-Seq of Aphaenogaster Ant
Polymerase Chain Reactions (PCRs) and sequencing was performed at Microbiome Insights (Vancouver, Canada). Bacterial 16S rRNA genes were PCR-amplified by focusing on the V4 region (515F 5-GTGCCAGCMGCCGCGGTAA-3, and 806R 5-GGACTACHVGGGTWTCTAAT-3) following the protocol of Kozich et al. (2013). Amplicons were sequenced with an Illumina MiSeq using the 300-bp paired-end kit (v.3).