RNA-Seq of HEK293T
HEK293T cells were cultured in medium containing DMEM with high glucose, GlutaMAX supplement, sodium pyruvate, 10% FBS, 25 mM HEPES pH 7.2-7.5, 1x MEM non-essential amino acids, 1x Pen/Strep, and 0.1 mM BME at 37C with 5% CO2.Total RNA was extracted with TRIzol Reagent for one replicate. Strand-specific cDNA was prepared from polyA-enriched mRNA, then 150bp paired-end sequenced on an Illumina NovaSeq platform to a depth of 30 million reads.