GSM8242690: Saline-water, rep 4; Mus musculus; RNA-Seq
Duodenal mRNA was isolated using Trizol (Invitrogen) following standard protocols. Quantification and integrity analysis of total RNA were performed by analysing 1 μl of each sample in an Agilent 2100 Bioanalyzer (Agilent RNA 6000 Nano Kit; Agilent). Total RNA strand-specific RNA libraries were generated using 150 ng of total RNA with the Illumina® Stranded RNA Prep Ligation kit with RiboZero Plus following the manufacturer's instructions. Libraries were sequenced on an Illumina NextSeq2000 (Illumina, Inc.) in paired-end mode with a read length of 2×50 bp.