PacBio HiFi data from Nemophila menziesii: sample BB_146_C5_F2
High molecular weight (HMW) genomic DNA (gDNA) was extracted from 0.7 g  of young leaves, vegetative buds, and floral buds (Sample No.BB 146 C5 F2) using the Nanobind Plant Nuclei Big DNA Kit as per the manufacturer's instructions (Pacific BioSciences - PacBio, Menlo Park, CA) with the following modification. We used nuclear isolation buffer supplemented with 350 mM Sorbitol to resuspend ground tissue and during the first wash of the nuclei pellet. The extracted HMW DNA was further purified using the high-salt-phenol-chloroform method (PacBio). The DNA purity was estimated using absorbance ratios (260/280 = 1.82 and 260/230 = 2.22) on the NanoDrop ND-1000 spectrophotometer. The final DNA yield (23 g) was quantified using the Quantus Fluorometer (QuantiFluor ONE dsDNA Dye assay; Promega, Madison, WI). The size distribution of the HMW DNA was estimated using the Femto Pulse system (Agilent, Santa Clara, CA), and found that 64% of the fragments were 100 Kb or longer.