RNA-seq of ARPE-19 mitotic cytosolic RNAs_1
RNA for each sample was used to prepare libraries using the KAPA RNA HyperPrep Kit with RiboErase (which removes rRNA) (KK8560, Roche/KAPA Biosystems, South Africa), with some modifications. In particular, we followed the instructions with the kit except that we used a 3x bead-based clean-up method to purify the RNA and a 7x bead-based clean-up method to purify the DNA with adapter sequences.