GSM7986055: PBMCs + CD34+ Tapestri; Homo sapiens; OTHER
Frozen PBMC's were thawed following 10X Genomics “Sample Preparation Demostrated Protocol”. Immunomagnetic selection of CD34+ cell population was then performed by means of “CD34 Microbead kit, human” (Miltenyi Biotec) following the protocol provided by the manufacturer. 100000 cells (a mix of PBMCs and CD34+ cells) were resuspended in cell buffer and loaded in the Tapestri Platform as recommended in manufacturer protocol. Samples were processed using the Tapestri® Single-Cell DNA Sequencing V2 (Mission Bio, San Francisco, California, USA) and the libraries were  sequenced on a NovaSeq 6000 using High Output Kit v2.5 chemistry (Illumina, San Diego, California, USA).