GSM7986077: MCF7 NF1-EV E2 Rep 3; Homo sapiens; RNA-Seq
Buffer RLT (from RNeasy kit) containing betamercaptoethanol was added to each well of cells. The mix of buffer RLT and cells was then added to an MPbio lysing matrix E tube. Cells were homogenized for 20 seconds in an MPbio homogenizer, then the supernatant was added to a Qia Shredder column. An equal volume of 70% ethanol was added to the flow through, and the entire volume of liquid added to an RNeasy column. RNA was extracted according to the manufacturer's instructions for the RNeasy kit (Qiagen). KAPA mRNA Hyperprep kit (v7.21)