GSM7426896: NCC034, scRNA-seq; Homo sapiens; RNA-Seq
PMBCs from 6 ICB-treated patients were extracted for single-cell RNA sequencing. Approximately 16,000 PBMCs were loaded onto the Chromium Controller (10x Genomics, San Francisco, CA, USA) for targeted recovery of 10,000 single cells. The cells were partitioned into nanoliter-scale Gel Bead-In Emulsions and individually barcoded. The 10x Genomics Chromium Single Cell 3′ Reagent Kit v3 (10x Genomics, San Francisco, CA, USA) was used for reverse transcription, cDNA amplification, and library construction of gene expression libraries according to the manufacturer's instructions. Library quality was assessed using BioAnalyzer 2100 with an Agilent High Sensitivity DNA Kit (Agilent Technologies, Inc., Santa Clara, CA, USA).