GSM6402443: PDS5A-dTAG, dTAG, rep1_HiC; Homo sapiens; Hi-C
Cells were crosslinked using 1% formaldehyde, followed by quenching with 125 mM glycine for 5 min.Cells were penetrated and nuclei restriction reaction was performed overnight using DpnI digested DNA fragments were filled with biotin at 37°C for 1hr, and proximally ligated at RT for 4hr. Then DNA was de-crosslinked and purified with ethanol precipitation. After shearing to 300-700bp fragments,the biotin tagged DNA was captured by streptavidin T1 beads. Hi-C libraries were prepared on streptavidin T1 beads using VAHTSTM Universal DNA Library Prep Kit for Illumina.After final PCR amplification, DNA could detach from beads.Size selection were perfromed with Agencourt AMPure XP beads from Beckman Coulter