RNA-seq of gullus gallus
Total RNA was isolated from the tissues using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturers instructions. The quantity and purity of total RNA were monitored by a NanoDrop 1000 (NanoDrop, Thermo Scientific, Waltham, MA, USA) and 1% formaldehyde agarose gel electrophoresis. Both library construction and sequencing were performed on the Illumina HiSeq platform (ANNOROAD, Beijing, China).