GSM7815470: HTR8_siYTHDC1-1_rep1; Homo sapiens; RNA-Seq
Total RNA from HTR-8/SVneo cells were extracted using TRIzol reagent (Invitrogen, CA, USA) following the manufacturer's instructions. For the RNA-seq, the sequencing library was prepared using an Illumina mRNA-seq sample preparation kit and was then subjected to paired-end sequencing on the Illumina HiSeq platform.                                                      For the RIP-seq, the sequencing library was prepared deploying NEBNext Ultra Directional RNA Library Prep kit (New England Biolabs, USA) and sequenced on the Illumina HiSeq 3000 platform. For the MeRIP-seq, the library was prepared by smart-seq method. Both the input samples without IP and the m6A IP samples were subjected to 150-bp, paired-end sequencing on an Illumina NovaSeq 6000 sequencer.                                                                                                    For the ChIPseq, input amounts of DNA was converted into high quality libraries for next-generation sequencing on the Illumina platform using the NEBNext Ultra II DNA Library Prep Kit. Bead based size selection was performed for final library size selection to obtain ChIP-seq libraries containing fragments of 250 bp to 500 bp.