GSM7592880: iniG, rep2; Ciona robusta; RNA-Seq
Approximately 150 embryos were collected at each time point in TRIZOL. RNA was precipitated using the phenol-chloroform method, followed by RNA Clean & Concentrator kit (Zymo) as per the manufacturer's instructions, including a 10-minute DNase treatment on the column. Stranded RNA libraries were constructed on an Apollo324 automated workstation using the PrepX PolyA mRNA isolation kit (Takara) following the manufacturer's protocol. Paired-end sequencing was performed on Illumina Novaseq platform (61bp).