GSM7057288: Type 2 spontaneous tumor sorted Microglia, Sample 1; Mus musculus; RNA-Seq
Phase separation in cells lysed in 1mL TRIzol Reagent (ThermoFisher catalog # 15596018) was induced with 200 µL chloroform. RNA was extracted from 350 µL of the aqueous phase using the miRNeasy Micro Kit (Qiagen catalog # 217084) on the QIAcube Connect (Qiagen) according to the manufacturer's protocol. RNA libraries for RNA-seq were prepared using SMART-Seq v4 Ultra Low Input RNA Kit  following manufacturer's protocols.