Illumina NovaSeq 6000 sequencing; The epigenetic landscape of human colorectal cancer - 10x dataset
The epigenetic landscape of human colorectal cancer - 10x dataset
Primary colonic tissues were obtained from the hospital. Tumoral tissues were processed according to Fujii et al (Fujii et al, 2015) with few modifications. Surgically resected specimens were reduced to a size of 3-5 mm, extensively washed with cold PBS and gentamicin (20microg/ml) and incubated with PBS-EDTA (2,5mM) rocking on a wheel for 1h at 4°C. After PBS-EDTA tissues sample was further washed with cold PBS and tumoral cells were released and collected by centrifuging at 400g 5' 4°C.  Not applicable scRNA-seq libraries were prepared according to 10X library preparation kit.