Methylome of Kordia algicida
DNA was extracted using the Quick-DNA HMW MagBead Kit (Zymo Research), sheared to a size of 7-10kb and a library prepared using the Pacbio SMRTbell Preparation Kit. After quality control of the library, the genomic DNA was sequenced using Pacbio Sequel IIe technology with a mutualised SMRTcell of 8M. Sequences were analysed using the SMRTLink Microbial Genome Analysis pipeline for de novo assembly of the genome and analysis of the 6mA and 4mC DNA sequence motifs.