scRNA-Seq of Mus musculus lymph node
Tumors or lymph nodes were dissociated and processed into single cell suspensions and sort-purified: P1: Tetramer+CD45+CD3+ (Antigen specific T-cells), P2: CD45+CD3+Tetramer- (polyclonal T-cells), P3: CD45+CD3− (Non-T-cell immune cells), and P4: CD45-. P1, P2, P3, and P4 were mixed at a 2:1:1:1 ratio and cells were encapsulated into droplets using 10x Chromium GEM and libraries prepared using the Single Cell 5′ Reagent Kit version 2.0 (10x Genomics) prior to sequencing using a NovaSeq instrument, as previously described (81).