Metatranscriptomics of microbiome samples
The total RNA was extracted using the DNA/RNA Miniprep Kit (Zymo Research Corporation) following the manufacturer instructions and stored at -80 after extraction. A fraction of purified RNA was subsequently reverse-transcribed to cDNA using random hexamer primers via Super-ScriptTM III Reverse Transcriptase (Invitrogen) following the manufacturers instructions, followed by the rRNA depletion using the Ribo-off rRNA Depletion Kit V2