16s rDNA amplicon-seq of phyllosphere bacteria
3 microliter of PCR product was resolved on 1.2 percent Agarose gel at 90V for approximately 60 min or till the sample reached 3/4th of the gel. The libraries were normalized and pooled to a final before being denatured and sequenced using paired 300-bp reads to generate high-quality, full-length reads of the V3 and V4 region.