GSM7589775: Wing disc, HWT, H3K27me3, rep2; Drosophila melanogaster; OTHER
A minimum of 20 wing imaginal discs per replicate were dissected from wandering female L3 larvae and used as input for CUT&RUN, as described previously (Ahmad 2018). The following antibody concentrations were used in CUT&RUN experiments: 1:100 anti-H3K9me3 (Abcam ab8898), 1:100 anti-HP1a (Developmental Studies Hybridoma Bank C1A9), 1:100 anti-GFP (Rockland 600-401-215), 1:100 anti-H3K27me3 (Cell Signal Technology #9733s), and 1:100 protein A/G-MNase. Using expression vector from Addgene (plasmid #123461), protein A/G-MNase was produced by the UNC Protein Expression and Purification Core using a published protocol (Meers 2019). Libraries were prepared using Takara ThruPLEX DNA-Seq Kit (R400675) with Takara DNA Unique Dual Index Kit (R400665R, R400666R, R400667R, R400668R).