GSM7770369: C1; Homo sapiens; RNA-Seq
From the differentiated spheroids, RNA was extracted using TRIzol Reagent (#10296028, Thermo Fisher Scientific) and purified using isopropanol and 75% ethanol. RNA samples were depleted DNase using TURBO DNA-freeTM Kit (#AM1907, Thermo Fisher Scientific), and purified to mRNA by Poly-A selection using NEBTNext Poly(A) mRNA Magnetic Isolation Module (#E7490, New England Biolabs). Only RNA samples with RIN > 6 were used to generate libraries. Following purification of first-strand cDNA, libraries were PCR amplified in accordance with their input RNA amount and purified using NEBNext Ultra II Directional RNA Library Prep Kit (#E7760, New England Biolabs).