GSM7592842: K562_dtagknockin_dtag13_12hr_CTCF_input_rep2; Homo sapiens; ChIP-Seq
Cells were fixed in 1% formaldehyde, washed, snap frozen, and stored at -80ºC. Cells were lysed and DNA was sonicated with a Branson Sonicator. Immunoprecipitations were carried out overnight for ChIP samples. ChIP or input DNA was used to generate sequencing libraries by end repair (End-It DNA repair kit, Epicentre), 3' A base overhang addition via Klenow fragment (NEB), and ligation of barcoded sequencing adapters.