GSM6403097: TgRH infected HFF_rep2; Homo sapiens; ATAC-seq
50,000 cells were resuspended in 50 µl of cold lysis buffer and centrifuged at 4°C for 10 min at 1000×g. The nuclei pellet was immediately resuspended in the transposition reaction mix and incubated for 30 min at 37°C. All transposed samples were purified using the Qiagen MiniElute Kit in 12 μl of buffer EB (10mM Tris-HCl, pH 8.0). Following purification, ATAC-seq libraries were amplified by PCR with NEBNext High-Fidelity 2x PCR Master Mix and 1.0 μM of Nextera Barcoded PCR primers (PCR conditions: one cycle of 72°C for 5min and 98°C for 30 sec; 10 cycles of 98°C for 10 sec, 63°C for 30 sec and 72°C for 1 min). All amplified libraries were purified using the Qiagen MiniElute Kit in 22 μl of buffer EB.