RNA-Seq of Lupinus luteus:  Cotyledon
RNA data were extracted separately, from three different tissues (leaf, hypocotyl, and cotyledon). According to the manufacturer's instructions, RNA extraction was performed separately with a Quick-RNA plant/seed kit (Zymo Research) from ~150 mg of fresh hypocotyl and cotyledon, and ~60 mg of young leaves. Then, the tissues were frozen with liquid nitrogen and powdered by grinding them in mortar. In addition, a 30 min DNAse digestion was performed. RNA concentration and A260/A280 were measured by absorbance in Synergy HTX multi-plate reader (Biotek). The integrity of RNA assessment was performed through 2% agarose gel electrophoresis run at 100V for 1 hour. PE150 sequencing was performed in the Illumina Novaseq 6000 platform