RNA-Seq of Schistosoma japonicum: adult male LNC3385 RNAi rep3
The integrity and quality of total RNA were examined by a Nanodrop ND-2000 spectrophotometer (Thermo Scientific Inc., USA) and an Agilent 2100 Bioanalyzer (Agilent, USA). Pooled RNA was used to construct a library using Illumina TruSeq RNA sample prep Kit (Illumina, San Diego, CA, USA) with Ribo-Zero Magnetic kit for rRNA depletion according to the TruSeq RNA Sample Preparation Guide.