ITS amplicon sequencing of freshwater metagenomes: mesocosm experiment
Amplicon sequencing was carried out following the workflow described by Buchner et al. (2021). The PCR for the amplicon library was performed using a two-step PCR protocol following Zizka et al. (2019). The libraries were then sequenced using the Illumina MiSeq platform with 2 lanes for each library with a paired-end kit (V3, 2x300).