GSM7815628: PD_4_C; Homo sapiens; ncRNA-Seq
Blood Plasma was isolated following the ThromboSeq protocol (Best et al. 2019). Plasma vesicles were immunocaptured from plasma, based on their membrane surface markers (CD61, CD9, CD63 or CD81), following a protocol (Fortunato et al. 2022) using magnetic beads (MACS beads, Miltenyi Biotec). Total RNA isolation was perfomed by using TRIzol LS reagent user guide with some modifications. Quality check of the isolated RNA was done with Agilent Bioanalyzer. RNA libraries for RNA-seq were prepared using (Bioo Scientific; cat no. NOVA-5132-05)