GSM8013041: Male-EPC-1_H3K9me3; Mus musculus; OTHER
Cells were washed with DPBS, counted, and 1×106 cells per vial were frozen in a freeze mix (90%FBS+10% Dimethyl sulfoxide-DMSO) and set aside in duplicates for CUT&TagTM sequencing. Approximately 2×105 nuclei were used for performing CUT&Tag-ITTM sequencing using a standard assay kit (Active Motif, USA cat no. 53160) for the H3K9me3 mark using a CHIP grade antibody (Cat no. 391691, Active Motif, USA). Further downstream steps were performed according to the protocol provided in the kit (Active Motif, 53160). DNA libraries were prepared using unique i7 and i5 index primers for each sample, size selected with SPRI beads (Cat No. 53160, Active Motif), quality checked with fragment analyzer, a capillary electrophoresis instrument for NGS library quality check and sequenced by the UMass Deep Sequencing Core with 25 bp paired-end Illumina Miseq system.