DArTseq of Bactrocera neohumeralis: whole body
DNA was extracted using the QIAGEN DNeasy Blood & Tissue Kit following the manufacturers protocol. Samples were screened for quality and quantity, using both gel electrophoresis and Qubit assay, and then sent to Diversity Arrays Technology Pty Ltd, Canberra (DArT P/L), for DArTseq high-density genotyping. A PstI/SphI, restriction enzyme combination was used by DArT P/L, and the fragments (up to 90bp) were sequenced on an Illumina Hiseq2500 as single end reads.