GSM7749724: CD4+ T cell, healthy, replicate 1; Homo sapiens; RNA-Seq
CD4+ cells expanded in vitro in the presence of Smith peptide were FACS sorted based on CD4+ and CellTrace dye dilution selecting the proliferated cells. Sorted cells were counted on a hemocytometer with 0.4% trypan blue. Single-cell concentration was adjusted to between 700-1200 single cells/μL  in 0.04% BSA/PBS and processed as per library construction protocol. Cells were loaded onto a 10x Chromium Controller (10x Genomics) to perform single-cell partitioning and barcoding. scRNA-seq libraries were constructed using 10x Genomics Chromium Single Cell Single Cell 5' R2-only chemistry according to the manufacturer's instructions (10x Genomics). Sample quality was determined by CE integrity analysis by AATI Fragment Analyzer and fluorimetric quantitation by Qubit (Invitrogen).