RNA-seq of Euglena gracilis: photoautotrophic growth at 22C in modified acid media and 25M CdCl2; sample was pretreated with elevated levels of sulfur prior to CdCl2 exposure; sampled after 8 days of CdCl2 exposure
Total RNA was isolated using TRIzol and treated with DNase I; Poly(A) mRNA was enriched using oligo dT-beads and cDNA libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina.