Metagenomic Seq of microbes: WAS
DNA extract was fragmented to an average size of about 400 bp using Covaris M236 (Gene Company Limited, China) for paired-end library construction. Paired-end library was constructed using NEXTFLEX Rapid DNA-Seq (Bioo Scientific, Austin, TX, USA). Adapters containing the full complement of sequencing primer hybridization sites were ligated to the blunt-end of fragments.