GSM8105615: 2-LSK-SLAM-H2BLo-MMPLo [G3-2]; Mus musculus; RNA-Seq
After culture, total RNA was isolated using tusing the Smart-seq v4 Ultra Low Input RNA Kit (Takara/Clontech) according to manufacturer's protocols. Total RNA was extracted from sorted HSC derived from each group using the Smart-seq v4 Ultra Low Input RNA Kit (Takara/Clontech).  RNA quality and concentration were measured by Bioanalyzer 2100 using the RNA 6000 Nano Assay (Agilent Technologies). Total RNA samples from HSC were used for cDNA amplification using Ovation RNA-seq v2 assay kit (Tecan Genomics) . The Illumina protocol, Nextera XT DNA Library Preparation Kit was used to create DNA library templates from double stranded cDNA. Paired-end sequencing using an Illumina NovaSeq 6000 SP Flow Cell was performed, at a target depth of 50 million paired-end reads.