PARE-Seq of Marchantia polymorpha: mature male vegetative thalli
Parallel Analysis of RNA Ends (PARE; RNA degradome) libraries were prepared from poly(A)-enriched RNA from following Marchantia polymorpha subsp. ruderalis organs: vegetative female thalli, archegoniophores, vegetative male thalli and antheridiophores. Preparation of each library was like previously described in the protocols (Addo-Quaye et al., 2009; German et al., 2009) with slight modifications (Grabowska et al., 2020; Sega et al., 2021).