GSM7728320: LA3_scRNA_seq; Mus musculus; RNA-Seq
Cells were loaded on to a 10x Genomics Chip G targeting 10,000 cells/sample and processed using the 10x Genomics NextGEM 3' v3.1 chemistry (PN 1000268). Libraries were prepared according to manufacturer's instructions and sequenced using NextSeq2000 paired-end 100 cycle kits (Illumina), targeting 25,000 reads/cell (Read1: 28 cycles; Read2: 90 cycles; Index1: 10 cycles; Index2: 10 cycles). After 7 days of infection, CARLIN mice (LA1, LA2, LA3) were euthanized, and their spleens were harvested. The spleens were mashed and filtered to get a single cell suspension. Next, Erythrocytes were depleted and the samples were enriched for CD8+ T cells. The enriched CD8+ T cells were stained and finally, live, OVA-Tetramer-BV421 and OVA-Tetramer-PE double positive CD8+ T cells were sorted.