Double digest Genotyping by Sequencing (GBS) library of Cucurbita radicans from Mexico
The seed was germinated in a greenhouse, DNA was extracted from fresh leaves using CTAB. DNA was sequenced by University of Minnesota Genomics Center. Two restriction enzymes (NspI and BfuCI/Sau3AI) were used. Genotyping by Sequencing (GBS) method was performed with an Illumina Novaseq instrument.