GSM7099720: captured mRNA-derived cDNA, 35-DAP seeds, rep 1, first experiment; Xanthomonas citri pv. fuscans; RNA-Seq
RNA was extracted using Trizol at 65°C. Depending on RNA quality, 100 to 200 ng of total RNA were used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using SureSelectXT RNA Direct technology (Agilent) according to supplier instructions. Probes (120 nt) for bacterial mRNA capture were designed from  annotated CFBP 7767 genomic sequence.