Botrytis cinerea treated with hydroxychloroquine for 12 hours, with a final concentration of EC50
The mycelia plug (diameter 4 mm) at the 3-day-old colony edge of strain CGMCC3.20932 was transferred into pdb medium, inoculated in an incubator at 25 for 3 days, represents Botrytis  strain CGMCC3.20932 treated with hydroxychloroquine for 12 hours, with a final concentration of EC50,and the mycelia was ground into fine powder under the protection of liquid nitrogen.   The RNA of the total sample was isolated and purified by TRIzol (thermofisher, 15596018) according to the operation scheme provided by the manufacturer. Then the quantity and purity of total RNA was controlled by NanoDrop ND-1000 (NanoDrop, Wilmington, DE, USA) and the integrity of RNA was detected by Bioanalyzer 2100 (Agilent, CA, USA). The concentration was >50ng/L, RIN value >7.0, and total RNA>8g.