Cell specific gene silencing of lipid-siRNA conjugates in the mouse central nervous system (CNS)
Cell specific gene silencing of lipid-siRNA conjugates in the mouse central nervous system (CNS)
Mice were perfused with heparinized (10U/ml) DPBS and a single cell suspension of the brain was prepared using the adult brain dissociation kit. Cd11b positive and negative cells were isolated via MACS with Cd11b-coated microbeads according to the manufacturer's instructions. After two washes with cell suspension buffer, cells were counted on a hemocytometer and diluted to target 2000 (Cd11b+) or 20,000 (Cd11b-) cells for encapsulation. Cells were encapsulated using the PIPseq method, v3 chemistry. The PIPseq manufacturer's guide was then followed for nucleic acid extraction. The PIPseq manufacturer's guide was then followed for library preparation.