Solanum dulcamara 39084 genome
Solanum dulcamara ID 39084 obtained from seeds provided by the Royal Botanical Gardens Kew (UK). Seeds were surface-sterilised in 1% bleach and rinsed and then stratified during 10 days on wet tissue paper mianteined at 4C and darkness. After that, they were moved to soil (John Innes No2) and kept in a growth room of the University of York with an average temperature of 20C (2C) and 14h/10h light /dark. The plants were self-pollinated to obtain berries. DNA was extracted from 3-month adult plant leaves using the High molecular weight gDNA extraction from plant leaves using the protocol described in doi:10.1101/783159. The gDNA quality was assessed with Nanodrop and Agilent 2100 Bioanalyser. The libraries were prepared using the SQK-LSK109 ligation sequencing kit and run with PromethION R9.4.1 (FLO-PRO002) flow cells