GSM8244168: Input_LC21_Rep1[ChIP_Seq]; Mus musculus; ChIP-Seq
B cells were treated with disuccinimidyl glutarate for 30 m and then washing with cold PBS before chromatin crosslinking by 1% formaldehyde in PBS at 25°C for 10 m.  Chromatin was sonicated to yield approximately 200–600-bp DNA fragments, pre-cleared with agarose beads conjugated with protein A (ThermoFisher) and then incubated with a polyclonal rabbit Ab to NF-kB p65. After incubation overnight, immune complexes were isolated using agarose-beads conjugated with protein A, washed and eluted in buffer.  Eluates were incubated at 65°C for 4 h to reverse formaldehyde crosslinks. DNA was purified using a QIAquick PCR purification column (Qiagen). Illumina-compatible index primers