GSM7986588: Human_islets_6hr_IL-1b_1; Homo sapiens; RNA-Seq
Following cytokine treatment, islets were dissociated into single cells and loaded on the Chromium Controller (10x Genomics). Single-cell RNA-seq libraries were prepared using the Chromium Single Cell 3′ v3 Reagent Kit (10x Genomics) for replicate 1 and the Chromium Next GEM Single Cell 3′ v3.1 Reagent Kit (10x Genomics) for replicates 2 and 3 according to the manufacturer's protocol. Libraries were sequenced using the NextSeq 500/550 High Output Kit v2.5 flow cell (150 cycles, Illumina) according to the 10x Genomics protocol. Samples were sequenced to a depth that returned approximately 30,000 reads per cell on average