GSM7848499: GR; Homo sapiens; OTHER
CUT&Tag was performed using NovoNGS CUT&Tag 3.0 High-Sensitivity Kit (for Illumina) (Novoprotein) according to manufacturer's instructions. In brief, Caki-1 cells were collected and bounded to Concanavalin A–coated beads. Subsequently, cells were resuspended in antibody buffer and incubated with primary antibodies against GR and secondary antibodies in order. The samples were incubated with pA-Tn5 transposase. After transposon activation and tagmentation, DNA was isolated, amplified, and purified to construct library. The library for sequencing was constructed and NovoNGS DNA Clean beads were used for purification steps. The library was quantified with NovoNGS CUT&Tag 3.0 High-Sensitivity Kit (for Illumina) and sequenced on an Illumina novaseq 150PE.