Pumpo (Bos taurus), a top bull from a Peruvian genetic nucleus
a genomic library with Illumina paired-end (2  150 bp) configuration was prepared according to Illumina's established procedures (Illumina, San Diego, CA, USA) and sequenced on an Illumina HiSeq 2500 system by GENEWIZ (South Plainfield, NJ, USA). The sequencing library underwent clustering on a flowcell, which was then installed in the Illumina sequencing instrument as per the manufacturer's directions. The Illumina Control Software was employed for image analysis and base calling. The raw sequencing data (.bcl files) procured from the sequencing process were converted into fastq format utilizing the Illumina bcl2fastq 2.17 software, with the protocol allowing for a single mismatch in the index sequence identification.