Vflo
Young leaf tissues of C. wilfordii, C. chinense, C. japonicum, and V. floribundum were collected from mature plants and snap-frozen in -80 C liquid nitrogen. Total RNA was extracted using a Maxwell 16 Cell LEV Total RNA Purification Kit (Promega), and their quantity and quality were assessed with Quant-IT RiboGreen (Invitrogen) and TapeStation RNA ScreenTape (Agilent), respectively. Only high-quality RNAs with RIN greater than 7.0 were used for RNA library construction. A library was independently prepared with 1 g of total RNA for each sample using a TruSeq Stranded mRNA Sample Prep Kit (Illumina).