GSM6738679: PCNSL_CNS_4282; Homo sapiens; RNA-Seq
high pure RNA Isolation Kit from Roche Life Science (11828665001) For library preparation RNA was converted to cDNA and amplified (10 PCR cycles) with the  SMART Seq v4 ultra-low RNA kit from Takara following the manufacturer´s instructions. For library preparation 10ng or the respective maximum available amount of RNA were used as input for the NEB ChIP Seq protocol following the manufacturer´s recommendations but skipping the size selection and using the high fidelity 2x PCR master mix from NEB. RNA-Seq, Two pools consisting of 7 libraries each were sequenced on HiSeq 4000 in 100 bp paired end mode, which produced an output of at least 48 Mio. Reads pairs per sample.