single-cell RNA-sequencing of tumors from PyMT;Shcbp1-/-
Tumors from PyMT;Shcbp1+/+ and PyMT;Shcbp1-/-mice were processed using a MACS Mouse Tumor Dissociation Kit (Miltenyi Biotec). The cells were counted using a cell counter (Countstar Rigel S2). Single-cell RNA-seq libraries were prepared using Chromium Next GEM Single Cell 3 Reagent Kits v3.1 (10 Genomics) and assessed using Qubit 4.0 and Agilent 2100. Sequencing was performed on an Illumina NovaSeq 6000 (150bp PE, >50,000 reads per cell) by the Biomarker Technologies Corporation (Beijing, China).