GSM7099985: del(xpk)_Dark_12hr_biol_rep_3; Synechococcus elongatus; RNA-Seq
For RNA extraction, the cell pellets were washed once with 300 µL of Qiagen RNAprotect® cell reagent, and the supernatant was removed after centrifugation. The cells were then once again frozen with liquid nitrogen and thawed by adding 350 µL of RLT solution from the Qiagen RNeasy kit. After 3 rounds of freeze-thaw-vortex steps, the samples were centrifuged at 15000g for 10 minutes. The supernatant was transferred to tubes and mixed with an equal volume of 100% ethanol before transferring to RNeasy mini-spin columns. The standard procedures of the Qiagen RNeasy kit were followed, and the Invitrogen TURBO DNA-free™ kit was used to remove DNA contamination. For RNA-seq library preparation, RNA quality control was performed using the Agilent fragment analyzer 5200. Library preparation and rRNA depletion were performed using the Illumina Ribo-Zero Plus rRNA depletion kit. All sequencing was performed on Illumina Nextseq 2000 with P2 flowcell, 300 cycles, and pair-end 150 bp. Standard Ribozero plus procedure