GSM7729968: Control-1; Homo sapiens; RNA-Seq
After isolation, a single cell was suspended in a lysis buffer using a mouth pipette. Reverse transcription was performed using a 25-nt oligo(dT) primer anchored with an 8-nt cell-specific barcode and 8-nt unique molecular identifiers (UMIs). The 3ʹ-terminals of the amplified cDNAs were purified using Dynabeads MyOne Streptavidin C1 beads (Thermo Fisher Scientific). Libraries were constructed using the KAPA Hyper Prep Kit (KAPA Biosystems)