GSM7730131: Ribo_WT2; Drosophila melanogaster; OTHER
Testes were dissected from 2-3-day-adult-males in ice-cold PBS with 100 µg/ml cycloheximide. Dissected testes were immediately frozen with liquid nitrogen and stored at −80°C. We prepared 600 pairs of testes per sample. We prepared two replicates from WT (y1 w1118) and Mettl1-KO (Mettl1KO1) males, respectively. Ribo-seq: preadenylated linkers with the antisense sequence of T7 promoter were ligated and footprints were amplified by in vitro transcription. RNA-seq: the libraries were prepared by a SEQuoia Express Standard RNA Library Prep Kit (Bio-Rad).