RNA-Seq of homo sapiens infected with HIV-1
RNA was extracted and purified using the Qiagen QIAamp Viral RNA Isolation kit. RNA was purified and three coding regions of HIV-1 were amplified using QuantaBio qScriptTM Flex cDNA Synthesis Kit. Each fragment corresponded to gag-p2/NCp7/p1/p6/, pol-PR/RT-(1657 bp fragment), pol-IN-(1114 bp fragment), and env-C2V3- (480 bp fragment). The amplified positive PCR products were cleaned using the QIAquickR PCR Purification Kit (Qiagen). The Nextera sample preparation kit (Illumina) was used to prepare libraries that were sequenced using the Illumina MiSeq platform.