GSM7100020: PMA; Homo sapiens; RNA-Seq
RNA was harvested using Trizol® Reagent (Invitrogen, USA) according to manufacturer instructions. The quantification of purified RNA was measured by using SimpliNano™ - Biochrom Spectrophotometers (Biochrom, MA, USA) and 1μg total RNA were used for library construction. RNA libraries for RNA-seq were prepared using g KAPA mRNA HyperPrep Kit (KAPA Biosystems, Roche, Basel, Switzerland) following manufacturer's protocols. The purification of PCR products was performed by using KAPA Pure Beads system and the library quality was determined by using the Qsep 100 DNA/RNA Analyzer (BiOptic Inc., Taiwan). The library was then sequenced on an illumina NovaSeq6000.