GSM8101934: LSK, HFD, H3K4me3, replicate 2, CUT&RUN; Mus musculus; OTHER
We used Cleavage under targets and Tagmentation (Cut-and-Tag), a chromatin profiling strategy in which antibody-targeted controlled integration of DNA sequencing adapters produces libraries for paired-end DNA sequencing. ChIP-seq libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina from New England Biolabs. Libraries were validated using the Agilent High Sensitivity DNA Kit.