GSM7214002: Shhcre;Lonp1, lung, E13.5, rep1, bulk RNA-seq; Mus musculus; RNA-Seq
Total RNA from embryonic or adult lungs were extracted by using Trizol (Invitrogen) and RNeasy Micro RNA extraction kit (Qiagen). For bulk RNAseq, cDNA libraries were constructed by using Illumina TruSeq RNA Library Prep Kit V2 (Illumina). For scRNA, single cell libraries were generated from sorted epithelial cells by using Chromium Single Cell 3' v3 kit (10X Genomics).