GSM8013084: HTO single cell RNA-seq on T cells from skin from Ramp1 KO and WT mice following 2 weeks of S. epi treatment; Mus musculus; OTHER
Skin CD8+ lymphocytes labeled with TotalSeqC hashtag antibodies (BioLegend) from S. epidermidis-associated mice (six Lck-Cre- Ramp1 fl/fl animals and six Lck Cre+ Ramp1 fl/fl animals) were sorted as DAPI- CD90.2+ TCRb+ CD4- CD8b+ TCRgd- CD49f- NK1.1- B220- CD11b- CD11c- MHCII-, using a Sony MA900 cell sorter. Libraries were constructed with the 10X Genomics 5' kit. All samples were pooled together, and approximately 15,000 sorted cells were loaded onto the Chromium Single Cell Controller (10X Genomics) to encapsulate cells into droplets. The library was prepared using a Chromium Single Cell 5' Reagent Kits v2 (10X Genomics) following manufacturer's instructions, and then sequenced on an Illumina Nextseq500 (Next Seq 500/550 High Output Kit v2, Illumina).