scRNAseq of pituitary from rat
Single-cell transcriptome 3' cDNA libraries were constructed using a V3.1 kit for a 10x Genomics Chromium Controller (10x Genomics Inc., USA) according to the instructions, with an expected capture of 10,000 cells/passage. The cDNA libraries were qualified by a Qubit 3.0 Fluorometer (Life Technologies, CA, USA) and an Agilent 2100 High Sensitivity DNA Assay Kit. Sequencing was performed on an Illumina NovaSeq 6000 PE150 platform.