Targeted nanopore sequencing of BAX circRNA-derived amplicons (EHEB cell line)
Total RNA from EHEB cell line was reversely transcribed using random hexamers. Next, multiple nested PCR assays using divergent primers, specific for each BAX exon, were performed. After library construction, 3rd generation (nanopore-based) sequencing was conducted using the MinION Mk1C platform and the Flongle adapter.