GSM7585834: arrhex_big_3; Arabidopsis thaliana; RNA-Seq
Small, slow-growing buds (approximately 0.1 cm in length) and bigger, fast-growing buds (approximately 0.5 cm in length) were collected from Col-0, arr1-4, and arr3,4,5,6,7,15 plants to characterize the transcriptomic changes associated with bud activation. Small and big buds were collected from inflorescences of ~1-2.5 cm. 10 buds from 5-10 plants were collected per biological replicate, and 6 biological replicates were collected per bud size and genotype. Total RNA was extracted using the Quick-RNA Plant Kit (Zymo Research, Cat # R2024). RNA libraries were prepared by GENEWIZ using PolyA Selection and the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®. Strand-specific, paired-end sequencing was performed on an Illumina NovaSeq using a 2x150 bp configuration.