DNA and RNA sequencing of ER+ PDXs
Poly-A+ mRNA was extracted from 1 µg total RNA, followed by fragmentation and first strand cDNA synthesis. The resultant cDNA was end-repaired, A-tailed and ligated with Illumina dual barcode adapters. Libraries were sequenced in groups of 70 on NovaSeq 6000 instrument using the S4 reagent kit (300 cycles) to generate 2 x 150 bp paired-end reads.