GSM8060395: srna_AID_CSR1_1mM_rep1; Caenorhabditis elegans; ncRNA-Seq
Worms were collected in TriReagent (Thermo Fisher Scientific) and were subjected to three freeze-thaw cycles. Phase separation was achieved via 1-bromo-3-chloropropane (BCP) and the aqueous phase was precipitated in isopropanol at -80°C for 2 hrs. To pellet RNA, samples were centrifuged at 21,000 x g for 30 min at 4°C. The pellet was washed three times in 75% ethanol then resuspended in water. Small RNA-seq libraries were made using the NEBNext Multiplex Small RNA Library Prep Set (NEB) according to manufacturer's instructions. All RNA libraries were sequenced on the NovaSeq6000 Sequencer (Illumina).