V3V4 amplicon sequencing of appendiceal microbiota
16S rRNA amplicon sequencing targeting the V3-V4 region. Amplicon libraries were generated following the Illumina protocol (https://support.illumina.com/documents/documentation/chemistry_documentation/16s/16s-metagenomic-library-prep-guide-15044223-b.pdf) with the exception that increased amount of 75 ng of DNA template was used in the amplicon PCR reaction. Amplicon libraries were quantified using Qubit fluorometer.