single cell RNA-Seq of homo sapiens: adult female peripheral B cells
After FACS sorting of CD19 positive B cells from PBMC of a patient with newly-onset SLE, 12,000 cells were counted and viability was confirmed to be >90%. Single-cell suspensions were loaded on a 10x Genomics Chromium single-cell 5 v2 chip to prepare the libraries according to the manufacturers protocol.