Microbiome of Amphipoda_120.1B
The V4 variable region of 16S SSU rRNA gene was targeted using the primer pair 515F-Y and 806R. PCR amplifications performed in duplicates to avoid PCR biases. PCR products were visualised on agarose gels through electrophoresis, purified using Agencourt AMPure XP beads, and libraries for sequencing were subsequently prepared using the Illumina NextEra XT DNA Library Preparation Kit. Samples were pooled in equimolar concentrations, and sequenced on an Illumina MiSeq platform in 2  300 bp mode (paired-end).