GBS of Juniperus maderensis from leaf tissue
500ng of DNA from each sample was digested using Pstl-HF (New England Biolabs, Ipswich, MA, USA) restriction enzyme (CTGCA|G). After ligation to the barcoded and common adapters, 5ul of each sample was pooled and purified using 1:1 AMpure beads (Beckman Coulter, Brea, CA, USA). Subsequently, 35ng of the purified product was used for PCR amplification to enrich the library