GSM8013168: nanos2; Nematostella vectensis; RNA-Seq
Whole juvenile was dissociated with collagenase.  Mechanical force was used to disrupt the tissues and obtain single-cell suspensions. Cell suspensions were washed with 0.1% BSA (bovine serum albumin), and cell viability and counts were assayed with a Cellometer X2 (Nexcelom). Cell suspensions were diluted to 1000 cells/ microliter and loaded into a 10x Genomics single cell platform using vs3 reagents. 10X Genomics Chromium v2/v3 chemistry according to manufacturer protocols