RNA-Seq of Plecia longiforceps: adult male
Total RNA was extracted using the RNeasy Mini Kit (Qiagen, Germany). Samples from four male adults were pooled. RNA integrity was checked using TapeStation (Agilent, USA). RNA-seq libraries were prepared using the TruSeq Stranded mRNA Library Prep Kit (Illumina, USA) and sequenced on an Illumina NovaSeq6000 platform at Macrogen, Korea.