HiC data from S orangutan
Fibroblast cell lines cultured, 5 million cells centrifuged, snap frozen with liquid nitrogen. Sent to UCSC on dry ice. HiC libraries prepared with Dovetail Omi-C Kit.Fragment sizes 150-1400 to 1600 bp included in lib. Pooled samples shipped to Hershey Genome Science Facility for sequencing on the Illumina NovaSeq 6000 instrument. Requested 400 million read pairs at 2x150bp/sample from the Genome Sciences Core at Hershey. For demultiplexing,bcl2fastq version 2.20.0 was used. A single NovaSeq S2 flow cell was used. The targeted library size was 300-1,000, but at the end it included fragments 150-1400(1600) bp.