DNA-Seq of garlic: Leaves
102 young leaves of garlic were collected and stored at 80 C. The genomic DNA of each sample was extracted by the CTAB method. The reference genome of garlic (Allium_sativum:GCA_014155895.1) was predicted by electronic digestion using the restriction enzyme digestion combination of HaeIII+ScaI, and the sequence with a length of 464-494 bp was defined as the SLAF tag according to the principle of enzyme digestion scheme selection. DNA fragments of the desired length (~500 bp) were recovered by electrophoresis using Covaris for random interruptions, and an adapter was added for cluster preparation. Finally, sequencing was performed using an Illumina HiSeq 2000 platform.