GSM7505838: Heart, Mito_1; Mus musculus; RNA-Seq
Mitochondria and remaining tissue fraction were isolated as previosly described (10.1007/978-1-4939-2257-4_11). RNA was isolated using the TRIzol in combination with RNeasy Mini Kit (Qiagen # 217004) combined with on-column DNase digestion (RNase-Free DNase Set, Qiagen #79254) according to manufacturer's instruction. 10ng of total RNA was used as input for Pico Input Mammalian (Takara Bio)