GSM6793123: LOB30IP, rep3; Zea mays; ChIP-Seq
Chromatin was sonicated using a Bioruptor Plus UCD-300 sonicator, immunoprecipated DNA was sonicated using a E220 focused-ultrasonicator (Covaris) Immunoprecipated DNA was sonicated to an average size of 200bp and end-repaired using an End-it DNA End repair kit (Epicentre, ER0720). Fragments were ligated with the adaptor and amplified using 15 cycles on the thermocycler using illumina primers (Yeasen, 12613ES02). Post amplification libraries were size selected at 250-450bp in length using SPRIselect beads from Beckman Coulter. Libraries were validated using the Agilent High Sensitivity DNA Kit.