Inhibitory effect of methanol on the growth of the Rhodotorula strain TO4
In the transcriptome analysis, TO2 was cultivated in GYF containing 1.75% v/v of methanol (the Tr group) or in methanol-free GYF (CK group) for 24 h. Then the cells were collected by centrifugation at 10000 rpm for 5 min at 4 C. Cell pellets were washed with sterilized water twice. Afterwards BGI Genomics Co., Ltd. (China) carried out the extraction of total RNA, construction of cDNA, and high throughput sequencing.