CUT&Tag of mus musculus: adult male testis
CUT&Tag was conducted following previously described procedures with minor modifications to the CUT & Tag assay (Vazyme, TD904)61. Approximately 4x10^5 spermatocytes isolated by STA-PUT method  were allowed to bind to ConA beads. The primary antibody was incubated with the beads-cell suspension on a rotator overnight at 4C. The following day, the secondary antibody was added and incubated for 60 min at room temperature, followed by incubation with pA/G-Tn5 transposome and tagmentation in an activating buffer supplemented with magnesium. Adaptor-ligated DNA fragments were extracted and PCR-amplified using indexing primers (TD202, Vazyme). The amplified product was purified with VAHTS DNA Clean Beads (N411-01, Vazyme), and the quantity and quality of the DNA library were assessed using a Qubit 4 Fluorometer and Bioanalyzer 2100 (Invitrogen), respectively. The libraries were sequenced on the Illumina sequencing platform by Genedenovo Biotechnology Co., Ltd (Guangzhou, China).