GSM6600318: T1462, Tumor Sample 5; Canis lupus familiaris; RNA-Seq
TRIzol (Invitrogen), was used to extract total RNA from 29 primary tumors. The RNA samples were cleaned using RNeasy cleanup (Qiagen), respectively. RNA was quantified on a NanoDrop Microvolume  Spectrophotometer and quality was assessed by Bioanalyzer (Agilent). The total RNA extracted from 29 samples were processed using Universal Plus™ mRNA-Seq library preparation kit with NuQuant® (product number: 0520-A01), following manufacturer's protocol, and the resulting cDNA library was sequenced on Illumina NovaSeq6000 to generate