GSM7770383: DN_23_004_625MOI_plusbooster; Homo sapiens; RNA-Seq
Leuokopaks were purchased from AllCells LLC and these were collected from healthy donors per standard protocols using mobilization with G-CSF+ Plerixafor. CD34+ cells were isolated from the leukopaks within 24 h by first removing the platelets using the LOVO cell Processing System (Fresenius Kabi). GMP grade reagents, buffers and columns for CD34 immunomagnetic selection were purchased from Milteny Biotec and the platelet washed cells were incubated for 30-35 min using the CD34 Reagent following which a subsequent wash for excess antibodies was performed on the LOVO. The washed and labelled cells were subject to immunomagnetic selection using the CliniMACS Plus instrument (Miltenyi Biotec) following which the cells were cryopreserved at a concentration of 5x106-1x107 cells/mL in CryoMACS 50 or 250 Bags (Miltenyi Biotec) for the gene edited drug product generation step. 10X Genomics Chromium Next GEM Single Cell 3' Gene Expression Reagent Kit