16S rRNA of fermented seaweed
Culture-independent analysis of the microbiota of the fresh sugar kelp (day 1) and spoiled sugar kelp (day 13) were conducted using DNA extracts prepared from the stomacher bags using the protocol described in Srensen et al. (2021). Briefly, the V3-V4 region of the 16S rRNA gene was targeted in an amplicon sequencing (Illumina, 2021). The resulting sequences were analyzed using the QIIME2 pipeline (Bolyen et al., 2019) with the SILVA 138.1 SSU Ref NR 99 database (Quast et al., 2013), following filtration and trimming of the reads with the amplicon region primers using RESCRIPt (Robeson et al., 2020).