GSM8244490: Differentiated Brown adipocyte, DMSO, Day7 Rep1; Mus musculus; RNA-Seq
Total RNA were extracted using Trizol reagenet according to the manufactural protocol Libraries were prepared according to Illumina's instructions accompanying the NEBNext Ultra RNA library Prep Kit for Illumina and purified using Beckman agencourt AMPure XP beads. Library were analyzed using agilent bioanalyzer 2100. The purified DNA was captured using TruSeq PE Cluster kit V4 for cluster generation. Libraries were sequenced on the  Illumina HiSeq X Ten machine following the manufacturer's protocols.