ITS2 sequencing of Symbiodiniaceae: Coral-algal symbionts
The ITS2 primers consisted of a forward primer 5'-GTGAATTGCAGAACTCCGTG-3' and reverse primer 5'CCTCCGCTTACTTATATGCTT-3' following Pochon et al. (2001). Thermal cycler conditions consisted of an initial incubation step at 95 C for 7 mins, followed by 30 cycles at 95 C, annealing at 59 C and extension at 72 C for 30 seconds each. The final elongation step was at 72 C for 7 minutes followed by a 4 C hold.