transcriptome sequencing of Pyrus communis:  stem tissue infected with B. dothidea
Ribosomal RNA was removed from 2 g total RNA of each sample using Ribo-ZeroTM rRNA Removal Kit (Epicentre, USA). First strand of cDNA was synthesized using random primers and the RNA strand was degraded by RNaseH. After synthesizing second strand of cDNA with dUTP, the double-stranded cDNA was connected to the sequencing adapter. The library fragments were purified using AMpure XP system (Beckman Coulter, USA) and enriched using Illumina PCR Primer Cocktail in a 15-cycle PCR reaction. The libraries were then sequenced on NovaSeq 6000 platform (Illumina)