GSM7592741: Colorectal Carcinoma patient 44 years old, Normal colonic mucosa; Homo sapiens; miRNA-Seq
Total RNA isolation was performed using the Qiagen AllPrep® DNA/RNA/miRNA Universal kit as per manufacturers' protocol. Elution was done in Nuclease free water. 1 µg total RNA  was proceeded for library preparation using the NEBNext® Small RNA Library Prep Set for Illumina®. Adapters in this kit are directly, and specifically, ligated to miRNAs. This was followed by reverse transcription to double stranded cDNA, PCR amplification, and pooled gel purification to generate a library product.