Illumina HiSeq 1000 paired end sequencing; Streptozotocin-induced Type 1 and 2 Diabetes Mellitus Mouse Models Show Different Functional, Cellular and Molecular Patterns of Diabetic Cardiomyopathy
Streptozotocin-induced Type 1 and 2 Diabetes Mellitus Mouse Models Show Different Functional, Cellular and Molecular Patterns of Diabetic Cardiomyopathy
10-week-old C57BL/6J mouse models of type 1 Diabetes mellitus were generated through a single high dose of streptozotocin (STZ, 200 mg/Kg). Mice were fed with a normal chow diet (NCD). C57BL/6J mouse models of type 2 Diabetes mellitus were generated through a 60 kcal% high-fat diet (HFD; D12492, Research Diets) for four weeks, starting at 6 weeks of age. During the fourth week, mice receive daily injections of low-dose STZ (40 mg/Kg) over four consecutive days. C57BL/6J Control mice were fed with a NCD. RNA was extracted from whole heart sections of CTRL, T1DM and T2DM mice starting from OCT embedded tissues Ten sections of 20µm of thickness were collected in 1.5 ml tubes and RNA extraction was performed using RNA Purification kit (Norgen) Libraries were generated using depleted RNA obtained from 1 µg total RNA by TruSeq Sample Preparation RNA Kit (Illumina, Inc., San Diego, CA, USA) according to the manufacturers protocol without further modifications