RNA-Seq of EVs released from Plasmodium falciparum infected red blood cells
Extracellular vesicles (EVs) were harvested from P. falciparum ring stage (12hpi) cultures. RNA was extracted using the NucleoSpin miRNA Kit (Macherey-Nagel) without size fractionation. Libraries from EV RNA samples were prepared by using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB #E7580) according to manufacturers instructions.