GSM8191192: UPF2_KD, ESC, Rep6; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNeasy Plus Mini kit (Qiagen), following the manufacturer's protocol. Ribosomal RNAs were removed using NEBNext® rRNA Depletion Kit v2 (New England Biolabs). The enriched mRNA was then used to make the library using the SMART-Seq® v4 PLUS Kit (Takara Bio), as per manufacturer's instructions. Libraries were sequenced (pair-end reads) with an Illumina NovaSeq 6000 platform for 100 cycles at the UCSD institute for Genomic Medicine (IGM) core.