Genome sequencing of Rhizobium sp. AG207R
For genomic DNA preparation, a single colony of AG207R from a freshly streaked Yeast mannitol (YM) agar (Himedia, India) plate was inoculated into 50-ml centrifuge tube containing 5 ml of YM broth (Himedia, India) and incubated using shaking incubator at 200 rpm, 30C for 3 days. The cells were harvested by centrifugation at 6,000 x g for 2 min at room temperature.  Genomic DNA was prepared using GF-1 bacterial DNA extraction kit (Vivantis, Malaysia). All procedures were performed following the manufacturers instructions.