GSM7986426: Sample 19_d11_iMCPosEC_S4 [scRNA-seq]; Homo sapiens; RNA-Seq
For bulk RNA-seq, total RNA was extracted from fresh frozen cell pellet samples using Qiasymphony RNA kit following manufacturer's instructions (Qiagen, Hilden, Germany). For scRNA-seq, single-cell RNA samples were prepared using the Chromium Next 10X genomics kits (10xgenomics, PN-1000128, PN-1000127) following the manufacture's protocols. For bulk RNA-seq, RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using the manufacturer's instructions (NEB, Ipswich, MA, USA). For scRNA-seq, RNA sequencing libraries were prepared using the dual index kit (10xgenomics, PN-100021310x following the manufacturer's protocols.