WA1 wildtype
The genomic DNA from parasites was isolated by using the QiAmp DNA Blood Mini kit (Qiagen cat:6950). The isolated genomic DNA was subjected to Whole Genome Sequencing (WGS) by Illumina sequencing platform at Yale Genomic facility. Briefly, 3 g of the isolated genomic DNA was sheared to 500 bp size and adenylated 3 ends. The fragments allowed for sequencing on an Illumina MiSeq flow cell to obtain 150 bp paired end reads with 50X coverage.