WGS of Case control isolate 23
Bacterial cells centrifuged, pellet resuspended with buffer, RNaseA and lysozyme, then lysis buffer and proteinase K added, extraction was done by heating. Charge Switch gDNA mini bacteria kit was used to recover DNA and used Nextera XT DNA library preparation kit. Nanodrop and Qubit was used for quality control and quantification. Illumina Miseq Desktop Sequencer using Miseq Reagent kit v3 for 600 cycles.