GSM8060289: Multiplexed WT-KO-TG TSC; Mus; RNA-Seq
Tumor cell suspensions were stained, respectively, with anti-mouse TotalSeq™-C0301, C0302, and C0303 Hashtag antibodies (Biolegend #155861, #155863, #155865) together with fluorochrome-labelled antibodies against CD45.2, CD3, CD4, and CD8 cell sorting. 30,000 T cells (CD45.2+ CD3+) and 10,000 total cells for each mouse genotype were sorted by FACS Melody (BD Biosciences) and pooled for multiplexed single-cell RNA and TCR repertoire sequencing by 10X genomics Chromium Single Cell 5' Reagent Chromium Single Cell Mouse TCR Amplification Kits. 10X genomics Chromium Single Cell 5' Reagent Chromium Single Cell Mouse TCR Amplification Kits. Single Cell 5' Reagent Chromium Single Cell Mouse TCR Amplification