GSM7505973: ctr1d polyA Rep2; Schizosaccharomyces pombe; RNA-Seq
RNA was isolated using TriReagent (Sigma-Aldrich, T9424) and precipitated using 2-propanol (Sigma-Aldrich). 10ug of this isolated RNA was used for DNase treatment using NEB DNase 1 (M0303), and purified using the RNA Clean and Concentrator Kit (Zymo Research, R1015/R1017). 1 ug of RNA samples were OligodT purified (NEB, E7490) and the recovered poly(A) selected RNA was used for cDNA library preparation using NEBNext Ultra II directional RNA library kit (NEB, E7760) following manufacturers library preparation protocol.