RNA-Seq of mollusk
Total RNA was isolated from mollusks Benedictia baicalensis (pools of five) using a Direct-zol RNA MiniPrep kit (Zymo Research). Libraries were prepared using the MGI Easy RNA Library Prep Kit in accordance with the manufacturer's protocols. For each sample, two independently cDNA libraries were prepared. The cDNA libraries were sequenced from both ends on a DNB platform using a DNBSEQ-400 NGS sequencer (MGI Tech Co., Ltd., China) using a DNBSEQ-G400RS High-throughput Sequencing Kit.