GSM7097806: PDX-sp-2 [TCR]; Mus musculus; OTHER
mCD45-hCD45+hCD2+hCD4+hCD8- cells were flow sorted from cell suspensions prepared from spleens and skins of recipient mice and collected into tubes containingPBSwith 0.04%BSA. The cell suspensions were loaded on a Chromium Connect Single Cell Liquid Handler (10X Genomics) and RNA-seq and V(D)J libraries were prepared using Chromium Next GEM Automated Single Cell 5' Kit, v2 (10X Genomics)