GSM7728605: DMSO_4hr_rep1; Homo sapiens; OTHER
Cells were fixed with 2% formaldehyde for 10 minutes and permeabilised with 0.0025% digitonin. Chromatin was digested by MNase and religated. Decrosslinking and DNA extraction was performed. Samples were sonicated using a S220 focused-ultrasonicator (Covaris). Tiled-Micro-Capture-C (Tiled-MCC) combines Micro-Capture-C (MCC) library preparation with oligonucleotide capture enrichment for deep, targeted sequencing of chromatin interactions within regions of interest. Details are described in the Methods section of the paper.