Single cell RNA-seq analysis of iWAT cells from Twist2-Cre; tdTomato mice
inguinal WATs from Twist2-Cre; tdTomato mice were washed with PBS, minced, and digested with type II collagenase (2 mg/ml) in KRBB containing 10 mM HEPES (pH 7.4) and 3% BSA for 1 hr at 37C. The sample was filtered through a 70 m cell strainer and the remaining cells were washed and pelleted by centrifugation at 500g for 5 min.  Tomato+ cells were sorted using S3e Cell Sorter equipped with a 561 nm wavelength laser.  Single cell libraries was created using the 10X Genomics Chromium Single Cell 3' Library Kit.