GSM8242234: Calvarial preosteoblasts, cKO, rep3; Mus musculus; RNA-Seq
Total RNA was isolated using a total RNA isolation kit (Omega Bio-Tek, Norcross, GA, USA), and mRNA is enriched by oligo (dT)-attached magnetic beads. Sequencing libraries were generated using Hieff NGS® MaxUpTM II Dual-model mRNA Library Prep Kit for Illumina (YEASEN, Shanghai, China). The sequencing library was then sequenced on a MGISEQ-2000 platform (BGI, Shenzhen, China) and 150 bp paired-end reads were generated.