GSM6796789: Ana.May.F; Capra hircus; miRNA-Seq
Total RNA was extracted from the secondary follicles using the RNAiso plus as the manufacturer's instructions (Takara Biotechnology Dalian, Co. Ltd., China). Total RNA per sample was used as input material for the small RNA library. Sequencing libraries were generated using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA.) following manufacturer's recommendations and index codes were added to attribute sequences to each sample. A total of 3 µg RNA per sample were used as the basis for small RNA library construction by sequencing company (Novogene, Beijing, China). The sRNA library was sequenced at Illumina HiSeq 2500 platform (Illumina, San Diego, CA, USA).