GSM6738785: NASH Liver cells, Western Diet 52w, rep E; Mus musculus; RNA-Seq
Cells were isolated based on enzymatic digestion followed by fluorescent antibody labeling for partial immunodepletion of ASGR1 and CD31 positive cells. Non-fluorescent singlets, negative for SyTOX live-dead stain were purified by FACS.   10x Genomics Chromium Single Cell 3' V2 solution Library was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics)