GSM7506282: SW620 cells, input, ChIP; Homo sapiens; ChIP-Seq
Cells were crosslinked with formaldehyde, and chromatin was fragmented by digestion with micrococcal nuclease and sonication. The final libraries were quantified using an Agilent 2100 Bioanalyzer (Agilent, USA) and KAPA kit (Cat no. KK4602, UK). The libraries were then sequenced on a Hiseq 2500 platform (Illumina, USA). Peaking calling was performed based on the filtered sequence data. The peaks were further annotated according to the reference genome.