Lake water 8 degrees repl. A5
For amplification of the V1-V2 hypervariable region of the 16S rRNA gene, we used PCR primers Fw (5- TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGAGMGTTYGATYMTGGCTCAG-3) and Rv (5-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGCTGCCTCCCGTAGGAGT-3).