Colonization and Dissemination of Klebsiella pneumoniae is Dependent on Dietary Carbohydrates
DNA was extracted from stool using the Qiagen DNeasy PowerSoil Pro kit. Shotgun libraries were generated from 7.5 ng DNA using IDT for Illumina unique dual indexes and Illumina DNA Prep Library Prep kit at 1:4 scale reaction volume. Equal volumes of library were pooled from every sample and sequenced using a 300 cycle Nano kit on the Illumina MiSeq. Libraries were then repooled based on the demultiplexing statistics of the MiSeq Nano run. Libraries were sequenced on an Illumina Novaseq 6000 v1.5 flow cell producing 2x150 bp paired-end reads.