RNA-seq of sorted mouse gdT cell populations based on TCR Vg chain and Ly6C status
RNA-seq of sorted mouse gdT cell populations based on TCR Vg chain and Ly6C status
Cells were FACS sorted (DAPI-CD3+TCRD+CD27+, then Vg1+ or Vg4+ followed by gating on Ly6C positive or negative) from pooled lymph nodes (inguinal, brachial and axillary) and spleens of 10 wildytpe female C57BL/6 mice per biological replicate raw sequence quality was assessed using FastQC v. 0.11.8, trimmed using FastP, trimmed sequences aligned to mouse genome using STAR, raw counts per gene determined using FeatureCounts v.1.6.4 RNA isolation was performed using Qiagen RNeasy Mini Kit (74106) according to manufacturer's instructions SMARTer Stranded Total RNA-seq v2 - Pico Input Mammalian (Takara)