GSM6523369: 4sU-seq MCMV 48hpi new A; Mus musculus; OTHER
Cells were lyzed using Trizol and total cellular RNA was isolated. 4sU labelled RNA was biotinylated using Biotin-HPDP followed by separation of new and old RNA using streptavidin-coated magnetic beads. Bead bound RNA was recovered via DTT treatment followed by RNA purification using Qiagen RNeasy MinElute columns. Sequencing libraries were prepared for both total and newly transcribed RNA (4sU-RNA) as described in Rutkowski, A.J. et al, Nat. Commun (2015).