=_xlfn.CONCAT("16s rRNA Seq of: ",A3)
DNA extraction from fecal samples is performed using the QIAamp PowerFecal Pro DNA Kit and the V4-V5 region of 16S rRNA- genes are PCR amplified using barcoded dual-index primers. Primer barcodes and sequences will be provided upon request. Illumina compatible libraries are generated using the Qiagen QIASeq 1-step amplicon kit  and sequencing is performed on the Illumina MiSeq platform in the Functional Genomics Facility at UChicago using a 2250 Paired End reads, generating 5,000-10,000 reads per sample.  Raw V4-V5 16S rRNA gene sequence data is demultiplexed and processed through the dada2 pipeline into Amplicon Sequence Variants (ASVs). ASVs are identified with the Bayesian RDP classifier up to the genus level.