Illumina HiSeq 1000 paired end sequencing; Multi-omics Study Reveals the Synergistic Anti-tumor Effects of Aspirin and Gemcitabine-based Chemotherapy on Pancreatic Cancer and the Underlying Mechanism
Multi-omics Study Reveals the Synergistic Anti-tumor Effects of Aspirin and Gemcitabine-based Chemotherapy on Pancreatic Cancer and the Underlying Mechanism
A total of 1 Âµg RNA per sample was extracted from pancreatic cancer cells using Trizol (Thermo Fisher Scientific). T BxPC-3 and PANC-1 cell lines were maintained in high-glucose DMEM.  The cultured cells were harvested 48 h after the drug treatment. First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase(RNase H-). Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities.   The mRNA was purified from total RNA using poly T oligo-attached magnetic beads.  After cDNA synthesis and conversion, the NEBNext Adaptor with a hairpin loop structure was ligated to prepare for hybridization.