GSM8012720: GEX_CD27_KO; Mus musculus; RNA-Seq
Stromal vascular fractions of VAT of 10 WT or CD27-/- mice were pooled and TCRb+ CD4+ cells were subsequently FACS-sorted Cells were loaded on the Chromium Controller (10x Genomics) and Single-cell RNA-seq libraries were prepared using the Chromium Single Cell 3ʹ v2 Reagent Kit (10x Genomics) according to manufacturer's protocol.