polyA+ RNA-Seq of C57BL/6xCBA Thumpd2 knockout mice
Mice line with Thumpd2 gene knockout was created by microinjection of Cas9 mRNA along with sgRNA targeting Thumpd2 gene into C57BL/6xCBA mice zygotes. Thumpd2+/- mice were back-crossed to the C57BL/6 genetic background and mated to obtain homozygous viable Thumpd2-/- mice. Total RNA from the mouse liver samples was extracted using QIAzol lysis reagent. NEB NEBNext Ultra II RNA Library Prep Kit for Illumina (E7770) was used for library preparation.