GSM8146591: mouse DRG, culture d7, rep1; Mus musculus; RNA-Seq
Total RNA was extracted from cell cultures using the Qiagen RNA Mini Kit. RNA quality was checked using a 2100 Bioanalyzer with the RNA 6000 Nano kit (Agilent Technologies). The RIN for all samples was ≥8.7. DNA libraries suitable for sequencing were prepared from 500 ng of total RNA with oligo-dT capture beads for poly-A-mRNA enrichment using the TruSeq Stranded mRNA Library Preparation Kit (Illumina) according to manufacturer's instructions (½ volume). After 15 cycles of PCR amplification, the size distribution of the barcoded DNA libraries was estimated ~325 bp by electrophoresis on Agilent DNA 1000 Bioanalyzer microfluidic chips.