DNA-seq
5 pooled transgenic plants each were amplified by PCR using Platinum SuperFi II DNA Polymerase (Invitrogen). The starting material for library preparation was 100 L of PCR products at 10 ngL1. Library preparation was conducted using the SQK-LSK110 ligation sequencing kit following the manufacturers protocol. Samples were sequenced using a minION Mk1c device using R9.4.1 (FLO-MIN106D) flow cells.