GSM8285339: Input for ChIPseq in NC350 endosperm Rep1; Zea mays; ChIP-Seq
For histone ChIPseq, chromatin was extracted as previously described (Villar and Köhler 2010). Briefly, tissue was crosslinked with 1% formaldehyde, ground and sheared with Covaris ultrasonicator. Mixture of Dynabeads with proteins A and G (1:1) (Invitrogen) was used for the IP and DNA was purified using ChIP Clean-up and Concentrator kit (Zymo Research). For TFs ChIP-seq, chromatin was extracted as previously described (Xu et al. 2021). Histone ChIPseq libraries were constructed using Ultra II DNA kit (NEB). TF ChIP-seq libraries were constructed with NEXTflex ChIPseq kit (PerkinElmer Applied Genomics)