GSM6600069: TIR-CAR-iMAC, scRNAseq; Homo sapiens; RNA-Seq
After co-incubation of CAR-iMACs with U87MGEGFRvIII cells for 24 hours, cells were harvested through trypsin digestion. DPBS was used to wash the cells  3 times to remove the residual medium.  Subsequently, the cells were processed with  10X Single Cell 3' Solution Reagents V2 to construct an RNA library. We used 10X Single Cell 3' Solution Reagents V2 following the manufacturer's protocol (10x Genomics)