GSM8061450: mesendoderm-Igf2_Igf2_dCTCFinICR-RNAseq-BS18543A; Mus musculus; RNA-Seq
Cells were dissociated using TryPE-Select, spun-down and media aspirate. RNA was isolated using the RNeasy-mini (QIAGEN 74106) protocol followed by DNase treatment using the Turbo DNA-free kit (ThermoFisher AM1907). RNA-seq libraries were prepared from 500 ng of total RNA using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB E7760S), and performing PolyA selection for ribosomal depletion using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB E7490) according to the manufacturer's protocol.