RNAseq of Haemonchus contortus fourth-stage larvae
Forth-stage larvae were generated from third stage larvae by exshethment and in vitro cultivation. Larvae were exposed to vehicle (DMSO), 100 nM ivermectin or moxidectin for 3, 6 or 12 hours. RNA was isolated using Necleospin RNA XS kit (Macherey-Nagel). RNA-Seq libraries were prepared and sequenced by a commercial service (Novaseq).