RNA-Seq of Chlamydomonas Sp.: S20exp
Next generation sequencing experiments were performed by Genomix4life S.R.L. (Baronissi, Salerno, Italy). RNA concentration in each sample was assayed with a Nanodrop One (Thermo Fisher) and its quality assessed with the TapeStation 4200 (Agilent Technologies). Indexed libraries were prepared from 700 ng/ea purified RNA with Illumina Stranded mRNA Prep according to the manufacturers instructions. Libraries were quantified using the TapeStation 4200 (Agilent Technologies) and Qubit fluorometer (Invitrogen Co.), then pooled such that each index-tagged sample was present in equimolar amounts, with final concentration of the pooled samples of 5 nM. The pooled samples were subject to cluster generation and sequencing using an Illumina Novaseq6000 System (Illumina) in a 2x101 paired-end format