GSM7730041: dKO, scTCRseq; Mus musculus; RNA-Seq
Hepatic CD4 T cells were sorted from NASH induced mice by flow cytometry. Library was generated using Chromium Next GEM Single Cell 5' Reagent kit v2 (10X GENOMICS). Sorted cells were resuspended in master mix and loaded to Chromium Next GEM Chip K, followed by microfluidic single cell partitioning and barcoding.  Transcribed cDNA was cleaned up and amplified.