GSM8242045: RNA-seq, hrde-1 (tm1200), rep2; Caenorhabditis elegans; RNA-Seq
RNA was extracted by using TRIZOL, phenol, and chloroform. DNase I was used to remove DNA. rRNA was depleted using anti-rRNA DNA oligoes and RNase H. For RNA ligation-based strategy, RNA-seq library was prepared using the protocol described in Ni. et. al. 2014 (PMID: 25534009). For SMARTer Stranded Total RNA-Seq strategy, RNA libraries were made by using the SMARTer Stranded Total RNA-Seq Kit (Takara). RNA ligation-based strategy and SMARTer Stranded Total RNA-Seq strategy