GSM7589224: Lymph_node_none_infect_Veh_rep4; Mus musculus; RNA-Seq
Draining lymph nodes were recovered in RLT plus buffer,homogenized using tissue lyzer. mRNA was extracted using Qiagen RNAeasy mini kit. The reverse transcription was performed using barcoded oligo-dT primers. The resulting cDNA from each sample was pooled into a single tube before proceeding to cDNA second strand synthesis. To generate Illumina-compatible libraries, the Unique Dual Indexed (UDI) library preparation method was employed following the previously described protocol (Alpern et al., 2019).