Transcriptome analysis of Foc TR4 challenged Musa spp.
The transcriptome library for sequencing was constructed according to NEBNext Ultra II RNA Library Prep Kit for Illumina protocol. The RNA Isolation was carried out using Banana tissues of Corm and Root according to the Conventional - Licl method (Phenol-Cholroform, Licl and Ethyl Alcohol) and Nanodrop (Q32851) (Thermo Fisher Scientific Massachusetts, USA) was used for RNA quantification. 500ng of purified RNA was taken for cDNA synthesis and amplification using a kit NEBNext Single Cell for cDNA Synthesis & Amplification Module (E6421), New England Biolabs Massachusetts, USA and cDNA was purified using Pronex beads (NG103B), Promega_madison, USA. cDNA library length was assessed using the tapestation instrument, Agilent Technology. The prepared library was sequenced using Illumina Novaseq 6000 platform, illumina, USA.