GSM7593099: HEK293T, circRNA, wt, rep 1; Homo sapiens; ncRNA-Seq
Total RNA was isolated using TRIzol reagent (Invitrogen, 15596018) or FastPure Cell/Tissue Total RNA Isolation Kit V2  (Vazyme Biotech Co., Ltd). Total RNA was treated with TURBO DNase (Invitrogen, AM2238) to remove Genomic DNA. The RNA was purified by RNA Clean& Concentrator Kits (Zymo Research, R1013) and then mRNA was isolated using oligo d(T)25 Magnetic Beads (NEB, S1419S). The supernatant polyA minus (polyA-) RNA was collected and purified by RNA Clean& Concentrator Kits (Zymo Research, R1013) After the digestion of linear RNAs with RNase R (Epicentre Technologies, USA) and removal of ribosomal RNAs using NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat), the resultant RNAs were regarded as circRNAs and subjected to generating sequencing library. Sequencing libraries were prepared with NEBNext® Ultra™ II Directional RNA Library Prep Kit (E7765).