Seawater at 21% salinity
The primers, 341F and 806R, were applied to amplify the V3-V4 regions of prokaryotic 16S rRNA gene from each extracted DNA sample. The processes of PCR and sequencing were performed at BIOZERON Biotech. Co., Ltd in Shanghai, China, using an Illumina Novaseq6000 platform with a 250 bp paired-end strategy.