GSM8322398: Yasmin_10x_010123_SPF; Mus musculus; RNA-Seq
The gingival tissues were excised. Tissues were then minced and treated with a Collagenase type II (2 mg/mL; Worthington Biochemicals) and DNase I (1 mg/mL; Sigma) solution in PBS plus 2% FCS for 25 min at 37 °C in a shaker bath. A total of 20 μL of 0.5 M EDTA per 2 mL sample was added to the digested tissues and incubated for an additional 10 min. The cells were then washed and filtered with 70-μM filter. Library was prepared using Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (Dual Index)