Genome sequencing of Carassius auratus ssp. 'Pengze': adult male muscle tissue
A total of 1.5g DNA per sample was used for DNA sample preparation. Sequencing libraries were generated using the TruSeq Nano DNA HT Sample Preparation Kit (Illumina USA) according to the manufacturer's recommendations, and index codes were added to attribute sequences to each sample. The DNA sample was sonicated to a size of 350bp, and DNA fragments were end-polished, A-tailed, and ligated with the full-length adapter for Illumina sequencing with further PCR amplification. PCR products were then purified using the AMPure XP system, and libraries were analyzed for size distribution by Agilent 2100 Bioanalyzer and quantified using real-time PCR. The libraries constructed above were sequenced using the Illumina NovaSeq platform, generating 150bp paired-end reads with an insert size of around 350bp.