GSM7586024: bladder carcinoma tissue27; Homo sapiens; RNA-Seq
25 pairs of cancer and peritumoral tissues, and the other 13 cancer tissues alone were pathologically confirmed before being frozeninliquidnitrogenand preserved at -80 ºC. Total RNA degradation and contamination of the frozen samples were monitored on 1% agarose gels. RNA purity was checked using the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA). RNA concentration was measured using Qubit® RNA Assay Kit in Qubit® 2.0 Fluorometer (Life Technologies, CA, USA). And, RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Sequencing libraries were generated using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA.) following the manufacturer's recommendations. The first-strand cDNA was synthesized using M-MuLV Reverse Transcriptase (RNase H– ). PCR amplification was performed using LongAmp Taq 2X Master Mix, SR Primer for Illumina, and index (X) primer. PCR products were purified and harvested.