Illumina HiSeq 2500 paired end sequencing; RNA-seq of human breast cancer cell lines characterized by ERalpha-LBD overexpression or knockdown, compared to control cells (with or without fulvestrant treatment)
RNA-seq of human breast cancer cell lines characterized by ERalpha-LBD overexpression or knockdown, compared to control cells (with or without fulvestrant treatment)
Cultured breast cancer cells were harvested and cell pellets were washed 3X with cold PBS solution before RNA extraction. Trizol / Chloroform extraction followed by isopropanol precipitation. Library preparation was performed by the sequencing contractor Genewiz (NJ, USA).