sequencing of human vaginal metagenome
Bacterial genomic DNA was extracted from swab samples using the PureLink Microbiome DNA Purification Kit, according to the manufacturers instructions. The quantity and purity of extracted DNA were measured with the Nanodrop ND-1000 spectrophotometer (Thermo Fisher Scientific, MA, USA). CM analysis was performed using the Ion Torrent technology, following the taxonomic identification of bacteria based on hypervariable  fragments of the 16S rRNA gene sequencing (Ion 16S Metagenomics Kit; Thermo Fisher Scientific, USA).