ddRAD of Phyciodes: adult thoracic tissue
Library preparation followed the ddRADseq approach of Peterson et al. (2012) (with PstI and MspI restriction enzymes) and was performed by the UAlberta Molecular Biology Service Unit. Demultiplexing was performed in the process_radtags program in Stacks v2.0b (Catchen et al. 2011, 2013). The PstI cutsite was removed using cutadapt v1.18 (Martin 2011). Reads that contained Illumina sequencing adapters were also removed and final cleaned sequences are 62 bp long.