ATAC-seq of mus musculus: adult male liver
ATAC-seq was performed as previously described with minor modifications (49). In brief, a total of 30,000 cells were lysed with 50 l of lysis buffer (10 mM Tris-HCl [pH 7.4], 10 mM NaCl, 3 mM MgCl2, 0.1% [vol/vol] Nonidet P40 Substitute), followed by Tn5 transposition with 50 l of transposition reaction mix (10 l of 5  TTBL buffer, 4 l of TTE mix and 36 l of nuclease-free H2O) from a TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme Biotech) at 37C for 30 min. The libraries were amplified using the TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme Biotech). Finally, libraries were sequenced on Illumina NovaSeq platforms with paired-end sequencing with 150-bp read length.