RNA-Seq of Cristatella mucedo: adult polypides
Total RNA was extracted using the Quick-RNA Microprep Kit (Zymo Research, Russia, R1050). The mRNA fraction was enriched using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England BioLabs Inc., Ipswich, Massachusetts, USA, E7490S). RNA libraries were created using NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) (New England BioLabs Inc., E7600S). Eighteen cDNA libraries were sequenced as paired-end, 100 base pair reads using Illumina HiSeq2500 instrument (Illumina, San Diego, CA, USA).