Small RNA-seq of Nicotiana benthamiana leaf agro-infiltrated with StRGA4 and Stu-miR482c: Replicate 2
Library preparation was performed at Bionivid Technology Pvt. Ltd. facility. 2.5 g of total RNA was used for the library preparation using NEBNext Small RNA Library Prep kit for Illumina (New England Biolabs). The library quality was assessed with Qubit 4.0 fluorometer (Thermofisher #Q33238) Tapestation 2200 (Agilent). Libraries then sequenced on an Illumina Hiseq-X sequencing platform and 51-bp single end reads were generated