GSM6600778: Ezh2KO_rep1 (KC_EZH2KO#2); Mus musculus; RNA-Seq
RNA was extracted from liver tissue  using RNeasy Plus Mini kit (Qiagen Cat#74134). Libraries were prepared from 200 ng of total RNA using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina from Lexogen, and optional UMIs (Vienna, Austria). Barcoded libraries were pooled, and single end sequenced (1X75) on the Illumina NextSeq 500 using the High output V2.5 kit (Illumina Inc., San Diego CA).