Raw data after adapter removal from Nanopore long-read sequencing:Cas12a-mediated bas4 mutant:Rep1-bas4#3
The DNA extractions for long-read nanopore sequencing were performed followed the online protocol (https://www.protocols.io/view/high-quality-dna-from-fungi-for-long-read-sequenci-k6qczdw). g-Tube (Covaris, catalog#520079) was used for shearing high-molecular-weight DNA into 20kb followed by AMPure XP beads purification (Beckman, catalog#NC9959336). The nanopore sequencing library preparation followed Native barcoding genomic DNA (with EXP-NBD104, EXPNBD114, and SQK-LSK109) protocol (https://community.nanoporetech.com/protocols/native-barcoding-genomic-dna/checklist_example.pdf).