GSM8147360: Pum2MM_N1 miRNA; Rattus norvegicus; miRNA-Seq
mirVana miRNA isolation kit with Total RNA Isolation Procedure Eukaryotic Small RNA Sequencing (Novogene), with 18-40 bp insert, small RNA library was prepared with the NEB Next® Multiplex Small RNA Library Prep Set for Illumina®  according to the manufacturer's instructions. Briefly, 3' and 5' adaptors were ligated to 3' and 5' end of small RNA, respectively. Then the first strand cDNA was synthesized after hybridazition with reverse transcription primer. The double-stranded cDNA library was generated through PCR enrichment. After purification and size selection, libraries with insertions between 18~40 bp were ready for sequencing with SE50.