GSM8146777: Cell culture, elicited, 24h, rep1; Morus alba; RNA-Seq
The Spectrum Plant Total RNA kit (Sigma-Aldrich, SKU: STRN250-1KT) was used to extract total RNA for Illumina sequencing. For PacBio sequencing, a 4 sample pool of different elicited samples was created. DNAse-treated RNA was monitored for its integrity using an Agilent Technologies 2100 Bioanalyzer defining high quality RNA if the RNA Integrity Number (RIN) value was greater than or equal to 7. For PacBio sequencing, cDNA synthesis was performed using Takara SMARTer PCR cDNA Synthesis Kit for ISOseq. Molecules under 4Kb were captured for library preparation. For Illumina sequencing, the TruSeq Stranded mRNA Library Construction kit was used to generate libraries.