GSM7815623: siControl_DMSO_control_1; Homo sapiens; OTHER
Total RNA was isolated using Trizol and resulting RNA samples mixed with in vitro transcribed 4sU incorporated spike-in RNA from selected RNAs of the ERCC RNA Spike-in Mix. Library preparation was carried out using the Lexogen QuantSeq 3' V2 kit using the manufacturer's protocol. 4sU-RNA was used for library preparation. The concentration and size distribution of the libraries were determined on a Bioanalyzer (Agilent Technologies). Alkylation of RNA was carried out using 10 mM iodoacetamide, and the reaction quenched with 1M DTT.