GSM6402463: PDS5B-dTAG, dTAG, rep3; Homo sapiens; ssRNA-seq
Total RNA was extracted from cells with Trizol reagent (Life Technologies). The RNA quality was assessed using the Agilent 2100 Bioanalyzer and mRNA was purified with VAHTS mRNA capture beads (Vazyme). Strand-specific RNA libraries were prepared with VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina (Vazyme)