GSM8242259: CH12F3_Ligase4-KO_Valemetostat_1; Mus musculus; RNA-Seq
Total RNA was isolated from indicated cells with RNeasy Plus Mini Kit (Qiagen, cat. no. 74134) and genomic DNA was removed by genomic DNA binding columns in the kit. Libraries were generated and sequenced by Novogene Corporation Inc. Briefly, Poly(A)-containing mRNA was purified from total RNA using poly(T) oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification; then was sequenced through Illumina NovaSeq (PE150) to obtain ~40 million paired-end reads (12G) per sample.