RNA seq of Peromyscus maniculatus: adult diaphragm
We used high-throughput RNA sequencing to identify gene expression differences among individuals exposed to different hypoxia (LH, PNH, AH) or normoxia (C) treatments. For each sample, Azenta Life Technologies (Plainsfield, NJ) extracted RNA from diaphragm tissue using a SMART-Seq HT kit for full-length cDNA synthesis and amplification, then prepared sequencing libraries using an Illumina Nextera X kit. After checking RNA and library quality using a TapeStation, Azenta performed 150 bp paired end sequencing on a HiSeq 4000 machine. Twelve control samples were prepared and sequenced together in one lane, followed by the remaining 27 samples prepared and sequenced on another lane.