Chromatin openness of common carp liver by ATAC-seq, replicate 2
Used the Nuclear Extraction Kit (Solarbio, SN0020) to extract the nuclei of two kinds of tissues for subsequent experiments. Then follow the manufacturers protocol, DNA was extracted from 40000-50000 nuclei by using TruePrepTM DNA Library Prep Kit V2 for Illumina (Vazyme TD501). Each prepared tissue DNA was sequenced with 150-bp paired-end reads on HiSeq X Ten. Other supporting reagents used in the experiment are TruePrepTM Index Kit V2 for Illumina (Vazyme TD202) and VAHTS DNA Clean Beads(Vazyme N411).