RNA-seq of R. kaschgarica
Fresh shoots of R. kaschgarica, including stems, leaves, and flowers, were sampled in Dulan County, Qinghai Province, China, which has an average annual precipitation of 179.1 mm. All materials were immediately frozen in liquid nitrogen and brought back to our laboratory for analysis. The stems, leaves, and flowers were pooled, and total RNA was extracted using a Plant Total RNA Kit (#DP432, TIANGEN, Beijing, China). The cDNA library was constructed following the description by Zhao et al. [41] and sequenced using the Illumina HiseqTM 4000 platform and 150 bp paired-end reads at Novogene (Beijing, China). A total of 6 Gbp of clean bases were generated, with 89.94% of the bases having a quality score of Q30 or higher.