GSM7730726: RNA_CBS5_KO_sample2_rep1; Homo sapiens; RNA-Seq
1 × 106 of cortex neurons or cultured cell were used for one experiment. Neuron cells were first dissected and digested using 0.00625% collagenase. Total RNA from culture cells was extracted using Trizol reagents (Life Technologies) following the manufacturer's instructions. Total mRNA was prepared from 1 mg total RNA using poly(A) mRNA magnetic isolation reagents (NEB) and fragmented at 94 °C for 15 min. RNA was then reverse-transcribed into cDNA with random primers. After end repairing and A-tailing, cDNA was ligated with adapters and amplified by PCR with Illumina sequencing primers.