GSM7058888: mesenteric artery ieCre Long term ID 8089; Mus musculus; RNA-Seq
Mesenteric arteries were collected without the attached intestine under RNase-free condition and stored immediately in RNAlater as previously described. (Simeone et al., Vascular gene expression in mice overexpressing human endothelin-1 targeted to the endothelium. Physiol Genomics. 2011, PMID: 21045115) The mesenteric arteries were dissected from perivascular fat and veins in RNAlater and then used for total RNA extraction in the same day. RNA samples were stored at -80°C until RNA-sequencing. Half microgram of RNA (n = 6 per group) was used for total RNA library construction using the TruSeq® StrndTotal RNA HT (w/Ribo-Zero Human/Mouse/Rat, Illumina, San Diego, CA) according to the manufacturer's protocol.