GSM6434451: H3K27Ac, ab4729, E14CTX, KCl, BioRep 2; Mus musculus; ChIP-Seq
Forty million cortical neurons were fixed with 1% formaldehyde for 10 minutes at RT followed by quenching and nuclei isolation. The chromatin was then sonicated using Misonix 3000 (Misonix) to obtain fragments of 200 bp to 2 kb and incubated with the H3K27Ac antibody (Abcam, #ab4729) for immunoprecipitation. Purified immunoprecipitated DNA was sent to BGI China for library construction and sequencing.