GSM6402556: liver, control-input, rep1-2; Sus scrofa; RIP-Seq
Total RNA was extracted with TRIZol reagent. Polyadenylated RNA was enriched by using PolyATtract® mRNA Isolation System III.Partial mRNA samples were saved as input control. A total of 5 µg fragmented mRNA was incubated with 12 µg anti-m6A antibody. Both the m6A IP samples and the input samples without IP were used for library generation with NEBNext® Ultra II Directional RNA Library Prep Kit. IP of the m6A RNA was performed with the GenSeqTM m6A RNA IP Kit following the manufacturer's instructions.