RNA-Seq of Megalopyge opercularis venom-producing tissue, preparation 3
Caterpillars for transcriptomic analysis were anaesthetised with carbon dioxide, and the dorsolateral exoskeleton dissected and stored in >10  volume of RNAlater (Ambion, Austin, Texas, USA) at 80C until analysis. Total RNA was extracted using the TRIzol (Life Technologies, Carlsbad, California, United States) according to the manufacturer's instructions. TruSeq libraries with 150 bp inserts were constructed and sequenced in paired-end format according to the manufacturer's instructions using a HiSeq 2500 instrument (Illumina, San Diego, CA, USA) by the Institute for Molecular Bioscience Sequencing Facility, The University of Queensland.