GSM7849064: right ventricular tissue, MCT3; Rattus norvegicus; ncRNA-Seq
Six right ventricular tissue samples from PAH and normal rats were used to obtain total RNA using the manufacturer's instructions and TRIzol (Invitrogen). Then, using a NanoDrop ND-1000 spectrophotometer, the amount of RNA was determined, and the integrity of the RNA was evaluated using denaturing agarose gel electrophoresis. The RNA samples were processed using the rtStar tsRNA Pretreatment kit (Cat# AS-FS-005, Arraystar) to eliminate changes like acetylation and methylation that might interfere with the construction of tiny RNA microarray libraries. To create a 3-OH end, whole RNA (100 ng) was first dephosphorylated. Following the manufacturer's recommendations, the labelled sample mixes were hybridized on an array using the Arraystar Small RNA Microarray. Total RNA of each sample was sequentially ligated to 3' and 5' small RNA adapters. cDNA was then synthesized and amplified using Illumina's proprietary RT primers and PCR finally, the completed libraries were quantified by Agilent 2100 Bioanalyzer. amplified fragments were extracted and purified from the PAGE gel. And amplification primers. Subsequently, ~134-160 bp