GSM7586170: Calu3_unsorted_Rep01; Homo sapiens; RNA-Seq
RNA was harvested using the NucleoSpin totalRNA FFPE micro kit (Macherey-Nagel #740969.50). To prepare sequencing libraries, we used the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490L) and NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770L). To index our samples, we used the NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) oligos (NEB E7600S). All sequencing was performed using an Illumina NextSeq 75 cycle high-output kit (Illumina 20024906) in a paired end format (38 cycles read 1 + 37 cycles read 2).