16s rRNA amplicon sequencing of human gut metagenome: hospital-acquired diarrhoea
Total DNA was isolated using Bioline ISOLATE II Fecal DNA Kit (Meridian Biosciences, London, United Kingdom). PCR was performed using Q5 DNA polymerase (New England Biolabs) with a primer set selected to amplify V3-V4 region of 16S rRNA gene. Sequencing was performed on an Illumina MiSeq (2 x 300 bp), following the method detailed by Fadrosh et al. 2014.