ChIP-seq of Homo sapiens: mCAT-HeLa cell
The immunoprecipitated DNA was used for ChIP-seq library preparation using the ThruPLEX DNA-Seq Kit (Takara Bio, R400674). The DNA was purified by Agencourt AMPure XP (Beckman Coulter). A final size selection was performed using 2% agarose E-Gel (Invitrogen), and the library was recovered by MinElute PCR Purification Kit (Qiagen).