RNA-Seq of PRL2023
Bifidobacterial cell pellets were resuspended in 1 ml of QIAzol lysis reagent (Qiagen, Germany) in a sterile tube containing glass beads. Cells were lysed by alternating 2 min of stirring the mix on a bead beater with 2 min of static cooling on ice. These steps were repeated three times. Lysed cells were centrifuged at 12,000 rpm for 15 min, and the upper phase was recovered. Bacterial RNA was subsequently purified using the RNeasy Mini Kit (Qiagen, Germany) following the manufacturers instructions. Then, the RNA concentration and purity were evaluated using a spectrophotometer (Eppendorf, Germany).