Illumina HiSeq 3000 paired end sequencing; RNA-seq with condition Rosiglitazone human adipocyte activated cells - role of PPAR alpha
RNA-seq with condition Rosiglitazone human adipocyte activated cells - role of PPAR alpha
hMADS adipocytes were transfected at day 10, with siRNA against PPAR alpha.   hMADS adipocytes were transfected with control siRNA (siGFP) at day 10. hMADS adipocytes were treated with transfection reagent only at day 10. hMADS white adipocytes were converted  to brown adipocytes at day 14 with 100 nM of Rosiglitazone for 4 days.  phenol chloroform extraction PolyA library construction