GSM7729921: hBMSC; Homo sapiens; RNA-Seq
P5 BMSCs and Day 8 BMSCs-derived neurospheres (both generated from the 41-year-old female donor) were dissociated into single cells by treatment with papain at 37℃ for 30 min. Dissociated cells were filtered through a 40 μm cell strainer with determination of cell concentration and viability (>98%) upon chamber slides following trypan blue staining. Cells were resuspended in ice-cold PBS and 0.04% bovine serum albumin at a density of 1,000 cells / μl. Resuspended BMSCs and BMSC-derived neurospheres were loaded onto the 10x Genomics Chromium platform according to the manufacturer's protocol, whilst scRNAseq libraries were prepared using the Chromium Single Cell 3ʹ GEM Library and Gel Bead Kit v3.