DNA-seq of Malus Domestica: Meristematic layer 1 of upper mutant branch of RubyMac
Trichome tissue (L1) was separated by freezing leaves in liquid nitrogen and dislodging the trichomes into a 50-mL Falcon tube using a soft paintbrush. The liquid nitrogen was allowed to boil off leaving the trichomes behind. DNA was extracted using the DNeasy Plant Mini Kit (Qiagen, Hilden, Germany). DNA samples were sequenced at The Max Planck Genome Center Cologne.