RNA-Seq of WT bulk
Equivalent amounts of leaf tissue from each of the 6 plants per pool were used for RNA extractions as described above, except that the final RNA preparations were obtained using the kit Nucleo-Spin RNA plant (Macherey-Nagel GmbH, Germany). The resulting RNA preps were evaluated using a Nano-Drop One and an Agilent 2100 bioanalyzer (Agilent Technologies, USA). All the samples showed RNA integrity numbers above 7.4. The six pools were sequenced (Macrogen Inc.) following library construction with the kit TruSeq Stranded mRNA LT (Illumina Inc.), in the NovaSeqTM 6000 platform (Illumina Inc.) with 151 bp paired read reads.