RNA seq of Locusta migratoria: nymphal and adult fat bodies
Fat bodies were collected from final 5th nymphal females at day 3 and from adult females at day 2 and 5. Each treatment consisted of three independent biological replicates, with each replicate consisting of a pool of fat bodies from four individuals. Total RNA was extracted using TRIzol reagent (Tiangen), and cDNA libraries were generated following the instructions of Illumina.