GSM6434187: neonatalDRG, CGM_96_93; Mus musculus; RNA-Seq
Neonatal and adult DRG and sciatic nerve total RNA was isolated using TRIzol reagent (Life Technology) and processed according to manufacturer's instructions. Neonatal and adult DRG and sciatic nerve total RNA was isolated using TRIzol reagent (Life Technology) and processed according to manufacturer's instructions. Quality of the extracted RNAs was assessed using the RNA 6000 Nano Kit on Agilent 2100 Bioanalyzer (Agilent Technologies). Libraries were prepared using the TruSeq Stranded mRNA Sample Prep Kit (Illumina) following the manufacturer's instructions. Sequencing of pooled dorsal root ganglia and peripheral nerves collected from young adult CGM, GF and OMM12 mice was performed on the Illumina NextSeq 500 platform. Sequencing of pooled dorsal root ganglia and peripheral nerves collected from neonatal CGM, GF and OMM12 mice was performed on the Illumina NextSeq 1000 platform.