GBS of Actinote mantiqueira : ALA SB-5
Total gDNA (200 ng) was digested with both high-fidelity enzymes PstI and MspI (New England Biolabs, Ipswich, MA, USA). Three libraries were prepared in total, each one with 96 pooled individuals. One of 96 barcoded adapters was attached to the PstI cut site for each sample and a common adapter (adapter 2) was attached to the MspI cut site of all samples using T4 ligase (New England Biolabs).