GSM8242244: Gene expression of Control, ICI, ICI-dexa10 and ICI-dexa200, scRNAseq; Mus musculus; RNA-Seq
Subcutaneous Hepa1-6 and orthotopic BNL 1MEA.7R.1 syngeneic HCC models were used. Low-dose (LD; 10 μg) or high-dose (HD; 200 μg) dexamethasone (Dexa) was intraperitoneally administered before each dose of anti-CTLA-4 and anti-PD-1. Tumor shrinkage, T cell priming, cytokine quantitation, as well as cytotoxicity and single-cell RNA-sequencing (scRNA-seq) of tumor-infiltrating T cells were assessed. Single-cell gene expression library was prepared by the Chromium Next GEM Single Cell 5ʹ Kit v2 (10x Genomics) following the manufacturer's instructions.