GSM8146861: RPE1, Pacbio; Homo sapiens; RNA-Seq
Total RNA from RPE-1 cells was extracted using TRIzol reagent (Life Technologies) following the manufacturer's protocol, and then was converted to cDNA using the Clontech SMARTer PCR cDNA Synthesis kit. Then we proceed to PCR cycle optimization, and use the cycle number to generate a sufficient amount of double stranded cDNA products. After purification of PCR products by using AMPure purification methods, SMRTbellTM template preparation was performed.