RNA-Seq of mus musculus: PCOS female feces
Library construction and sequencing: Total DNA were extracted from samples. Specific primers with barcode were designed to target on full-length amplicon. Target sequences were amplified by PCR and its products were purified, quantified and homogenized to generate SMRT bell library. Library QC was performed on the libraries and the qualified ones were processed for sequencing on PacBio Sequel platform. Pacbio Sequel initially generated files in bam format, which were further converted into CCS files by smrtlink. These sequences were identified by barcode sequences, which resulted in fastq sequences file of each sample.