Shotgun sequencing AD microbiome: acetate polyaniline-coated plastic materials first replicate
Library strategy: metagenomics. Genomic DNA was isolated and purified using the DNeasy PowerSoil (QIAGEN 181 GmbH, Hilden, Germany). Genomic DNA quality and quantity were determined using Qubit 4 Flurometer (ThermoFisher Scientific). Illumina libraries were prepared with the Nextera DNA Flex Library Prep kit (Illumina Inc., San Diego CA) and sequenced on the Illumina NovaSeq platform producing 150 bp paired-end reads