RNA-Seq of Homo sapiens: coronary artery
Each graded arterial specimen (100 mg tissue) was homogenized for total RNA extraction with TRIzol Plus RNA Purification Kits (Thermo Fischer Scientific).  Poly(A) mRNA was isolated using Dynabeads mRNA DIRECT Micro Kits (Life Technology).  The isolated mRNA was used to generate cDNA libraries using the Ion RNA-Seq Kit v2 (Life Technology).  The libraries were quantified with Experion Automated Electrophoresis System (Bio-Rad Laboratories) and normalized prior to template preparation for sequencing using the Ion OneTouch 2 System (Life Technologies). Multiplexed libraries were sequenced by the Ion Proton System (Life Technologies) producing 100 bp reads.