GSM8060305: N2_atac_1; Caenorhabditis elegans; ATAC-seq
ATAC-seq was performed as in Zhong et al. 2021 (doi: 10.1073/pnas.2023347118). Briefly, worms were ground and filtered through 40 um mesh to isolate nuclei, all on ice, then pelleted at 10 min 3000g. Extracted nuclei were treated with Tn5 transposase in a mix containing 25 uL 2x dimethylformamide + 2.5 uL Tn5 and 22.5 uL nuclei suspension at 37 C 30 min, then purified with a Zymo ChIP DNA Clean & Concentrator kit. Tagmented DNA was amplified using Phusion polymerase (NEB).