GSM8322376: SCWOPD, scRNAseq [SCWOPD-3]; Homo sapiens; RNA-Seq
The organoids were dissociated into single cells using the STEMdiff Cardiomyocyte Dissociation Kit (Stemcell Technologies, #05025). The cell suspension was then loaded into Chromium microfluidic chips for single-cell RNA sequencing. The cell suspension was processed with 3' (v2 or v3, depending on the project) chemistry and barcoded using a 10× Chromium Controller (10X Genomics). RNA from the barcoded cells was reverse-transcribed, and sequencing libraries were constructed using the Chromium Single Cell 3' v2 (v2 or v3, depending on the project) reagent kit (10X Genomics), following the manufacturer's instructions.