GSM8061586: D374Y mCherry-APOB; Mus musculus; RNA-Seq
live CD45+ mCherry+ cells were sorted from the liver. A small sample from one lobe was cut into small pieces and digested in 0.2 mg/mL collagenase IV for 30 min at 37 °C in RPMI 1640. The cell suspension was passed through an 18 g syringe 10 times to remove any clumps and then a 70uM cell strainer. The cell suspension was then washed with FACS buffer before being sorted by a Sony SH800S cell sorter. Cells were additionlay stained with Total-seq cell hashing antibodies from Biolegend. A0301 defines this liver sample Cells were processed immediately for GEM generation and barcoding on a 10X Chromium using Next GEM 3' v3.1 reagents (10X Genomics) followed by sequencing and processing on Cellranger. equenced on a NovaSeq 6000 on an SP flow cell with the following run set up: Run setup 28-8-0-91 , 1% Phix.