genome survey of Ilex pubescens: fresh leaves
High-quality genomic DNA samples were randomly fragmented by Covaris Technology, and the fragment of 350 bp was obtained after fragment selection. An A base was added at the 3-end of each strand for end repair. Then, adapters were ligated to both ends of the DNA fragments, followed by amplification by ligation-mediated PCR (LM-PCR), single-strand separation, and cyclization. DNA Nanoballs (DNBs) were produced through rolling circle amplification (RCA). The qualified DNBs were loaded into the patterned nanoarrays, and 150bp pair-end reads were read through on the MGISEQ-2000RS platform (BGI, Shenzhen, China) at the Compass Agritechnology Co., LtdBeijing, China.