Targeted nanopore sequencing of BCL2L12 circRNA-derived amplicons (U937 cell line)
Total RNA from U937 cell line was reversely transcribed using random hexamers. Next, multiple nested PCR assays using divergent primers, specific for each BCL2L12 exon, were performed. After library construction, 3rd generation (nanopore-based) sequencing was conducted using the MinION Mk1C platform and the Flongle adapter.