GSM7787315: 2-cell embryos, siAcox1 injection, ChIP-Seq, rep1; Mus musculus; ChIP-Seq
1. Cells were collected and counted at room temperature. 2. Take the required number of cells and centrifuge them in a 1.5ml EP tube at a low speed of 2,500 rpm(600 × g) at room temperature for 5 min, then discard the supernatant. 3. Add 100 μl pre-cooled NE Buffer（TD903，Vazyme） to each sample, gently blow the suspended cells with a pipette, and incubate on ice for 10 min. 4. Centrifuge at 2500rpm (600 × g) at low speed for 5 min at room temperature and discard the supernatant. 5. 100μl Wash Buffer was added to each sample. CHIP-seq libraries were prepared using the Hyperactive Universal CUT&Tag Assay Kit for Illumina(TD903，Vazyme).