GSM6701681: DN_rep2_RNA; Mus musculus; RNA-Seq
Briefly, a single cell suspension from the thymus or the spleen was prepared, then pre-enriched using EasySep Mouse CD4+ T cell Isolation Kit (STEMCELL Technologies, Cat# 19852), and stained with fluorescent-conjugated primary antibodies on ice for 30 minutes. The individual cell population was sorted by FACS. 1×104 cells were FACS-sorted into TRIzol RNA isolation reagent (Invitrogen) and RNA was isolated according to the protocol. RNA-seq libraries were constructed using Illumina TruSeq Stranded mRNA kit (Illumina) following manufacturer's instructions.