GSM6433940: ALK10_6_E775_Mesp1KO_rep2; Mus musculus; ATAC-seq
Dissociate whole mouse embryos in 0.25% TrypLE and quench with PBS+1%FBS to single cell suspension, perform nuclear lysis. Treat 10000 singularized nuclei with transposition enzyme. Load nuclei in 10X Chromium chip. 10X Genomics Chromium scATAC Library & Gel Bead Kit v1 used for library preparation according to manufacturer's protocol. single cell ATAC-seq