GSM7677483: LD, Yorkshire(P0), scRNA-seq; Sus scrofa; RNA-Seq
The longissimus dorsi muscle (LD) was isolated and obtained for 1g per piglet. Single-cell RNA sequencing (scRNA-seq) was performed on the 2.5IW cells obtained from the LD. To eliminate red blood cells and dead cells, the single cells were treated with Red Blood Cell Lysis Buffer and then subjected to magnetic bead separation. The viability of the filtered single cells was assessed using trypan blue staining (Thermo Fisher Scientific, Waltham, MA, USA) and haemocytometry (Thermo Fisher Scientific). library preparation for reverse transcription was performed using the 10x Genomics Single Cell v2 kit, following the specified protocol. The library was sequenced in a single lane on an Illumina NextSeq-500 system using the high-output (400 M) kit.