GSM7908552: YJM308 unstressed Rep3; Saccharomyces cerevisiae; RNA-Seq
RNA was extracted by the hot phenol method from Gasch, Methods in Enzymology, 2002, followed by DNase I (Ambion) digestion followed by cleanup with a Quick-RNA MiniPrep Plus Kit (Zymo Research) including optional on-column DNase digestion. RNA-seq libraries were prepared using the KAPA mRNA HyperPrep Kit (Roche) and KAPA Single-Indexed Adapter Set A + B, according to manufacturer specifications. 500 ng of total RNA was used as starting material for each sample, polyA-enrichment was performed, mRNA was fragmented to an average size of 200–300 nucleotides, and the cDNA libraries were amplified for 9 PCR cycles.