GSM7849223: LNCaP_KO_3; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol reagent and Zymo Research Total RNA extraction kit  and quantified using nanodrop and Agilent bioanalyzer. Total RNA samples were used for sequencing library preparation with KAPA RNA HyperPrep kit with RiboErase (HMR) following KAPA RNA library preparation protocol. Each library was bar-coded and then pooled for sequencing run paired-end sequencing protocol.