GSM7426629: dbr1_ntc_a; Homo sapiens; RNA-Seq
RNA was purified from cell lines using the Qiagen miRNeasy mini kit to accomodate small RNA isolation (#217004). For RNA isolation from mouse cortex, cerebral cortex was dissected in ice-cold PBS from 5 female Ttdn1-/- and 5 WT littermates at 8 weeks of age, then flash frozen. RNA was purified from cortex using the Qiagen miRNeasy mini kit. Samples for small RNA-sequencing were prepped with TruSeq Small RNA library preparations kits; otherwise all other samples were prepared according to library kit manufacturer's protocol, indexed, pooled, and sequenced on an Illumina NovaSeq 6000 2x150bp with the Genome Technology Access Center at Washington University in St. Louis, typically yielding 200 million paired-end reads per sample.