16s rRNA amplicon sequencing of human gut metagenome: faecal microbiota transplant donors
Total DNA was isolated using Bioline ISOLATE II Fecal DNA Kit (Meridian Biosciences, London, United Kingdom). PCR was performed using Q5 DNA polymerase (New England Biolabs) with a primer set selected to amplify V3-V4 region of 16S rRNA gene. Sequencing was performed on an Illumina MiSeq (2 x 300 bp), following the method detailed by Fadrosh et al. 2014.