GSM7099051: C57 mouse lung tissue miRNA, Control 2; Mus musculus; miRNA-Seq
Exosomes from three sham group and three CLP groups were used to perform RNA sequencing. MiRNeasy Serum/Plasma Advanced Kit (Qiagen, cat. No. 217204) was used for the extraction and purification of total RNA from lung tissue exosomes according to the instructions of the kit. For the small RNA libraries, the total RNA amount of each sample is 3ng, which is used as the input material for RNA sample preparation. According to the manufacturer's recommendations, the QIAseq miRNA library kit (Qiagen, Frederick, MD) was used to generate the sequencing library, and add the index codes to the attribute sequences of each sample. Reverse transcription (RT) primers with unique molecular index (UMI) were introduced to analyze the quantification of miRNA expression during cDNA synthesis and PCR amplification