V3 Plasmablasts BCR
Sorted plasmablasts and B-cell samples were processed using 10x Genomics kits (Next GEM Single Cell 5 GEM Kit v2 Dual Index (PN-1000244), Chromium Next GEM Chip K Single Cell Kit (PN-1000286), Chromium Single Cell Human BCR Amplification Kit (PN-1000253), Library Construction kit (PN-1000190), and Dual Index Kit TT Set A (PN-1000215)) according to the manufacturer's protocols. We used cDNA to prepare B cell receptor (BCR) and 5 gene expression libraries. All quantifications were performed on a TapeStation 2200 (Agilent, Santa Clara, CA, USA). Libraries were sequenced on a NovaSeq 6000 at sequencing read depths of 5,000 and 50,000 for VDJ and gene expression libraries, respectively.