CapTrap-Seq: A platform-agnostic and quantitative approach for high-fidelity full-length RNA transcript sequencing
CapTrap-Seq: A platform-agnostic and quantitative approach for high-fidelity full-length RNA transcript sequencing
commercial samples obtained from Ambion: Ambion is a brand of life science research products and technologies, mainly focused on RNA products. It is owned by Thermo Fisher Scientific, a company that provides a variety of scientific research products and services. Therefore, \"total RNA commercial samples obtained from Ambion\" refers to RNA samples purchased from this brand for research purposes. SMARTer cDNA library preparation: SMARTer cDNA library preparation is a protocol for long-read sequencing that involves the addition of a poly(A) tail to RNA, followed by reverse transcription using oligo(dT) primers. This results in the synthesis of cDNA with a 5' end that contains a known sequence, which is used for further amplification and sequencing. The SMARTer protocol also incorporates a template switching oligo, which allows for the amplification of full-length cDNA molecules and increases the efficiency of library preparation.