GSM8060612: As4.1 cells, H4K5ac, rep2; Mus musculus; OTHER
Cells were washed in dPBS and trypsinized at 37°C until cells detached. Detached cells were then suspended in dPBS with a protease inhibitor added. For each primary antibody, 100k cells were isolated and resuspended in Concanavalin A-coated beads. Primary antibodies to each histone modification were then added, with the guinea pig anti-Rabbit IgG used as the secondary antibody.  Tagmentation was performed using the pAG-Tn5 transposase.  Processed libraries were sent to Novogene (Durham, NC) for sequencing.