GSM8013412: rMSC cultured on wave-forming nanotextured titanium disc for 1 day under growth medium; Rattus norvegicus; ATAC-seq
Transposition reaction was performed using Illumina Tagment DNA TDE1 Enzyme and Buffer Kits, followed by DNA purification was proceeded using MinElute PCR purification Kit (Qiagen). Amplify transposed DNA fragment. To reduce GC and size bias in PCR, the appropriate number of cycles is determined using qPCR. Run a qPCR side reaction.Calculate the additional number of cycles needed, plot liner Rn versus cycle and determine the cycle number that corresponds to 1/4 of maximum fluorescent intensity. Run the remaining PCR reaction to the cycle number determined by qPCR. Purify amplified library. ThepurifiedlibrarieswerequantifiedusingqPCRaccordingtotheqPCRQuantificationProtocol Guide (KAPA)andqualifiedusingBioanalyzer (Agilenttechnologies). And then the libraries were sequencedusingHiSeq platform (Illumina).