GSM6434272: C1-022-C62_S29; Homo sapiens; RNA-Seq
CD45 live cells were captured using C1 single cell system (Fluidigm) and processed for whole transcriptome library preparation.  polyA RNA from each single cell was reverse transcribed and cDNA amplified using SMARTer kit (SMARTer Ultra Low RNA Kit for the Fluidigm C1 System,Clontech#634936) Amplified cDNA was used as input to prepare sequencing libraries with the NexteraXT DNA Kit (Illumina #fc-131-1096) and with dual indices (PN FC-131-1002; Illumina) RNA-Seq, paired-end NextSeq 74bp sequencing