GSM7506667: 2hr_rep2; Homo sapiens; RNA-Seq
Samples in Trizol were thawed, and ERCC spike-in RNAs (Thermo Scientific 4456740) were added at 10 L per million cells. RNA was isolated using chloroform following the MaXtract tube protocol (Qiagen 129056). 5 g of RNA was treated with DNaseI (Thermo Scientific 18068015) in the presence of SUPERase-In (Thermo AM2696). RNA quality was evaluated by HS RNA ScreenTape (Agilent 5067-5579) on a TapeStation; all samples had RIN score > 8. 500 ng RNA was used as input for the TruSeq Stranded Total RNA sequencing kit with RiboZero rRNA depletion (Illumina 20020598).