GSM7592986: tumor tissues, CM-ZIF8 at Ce6/Lon+L, rep2; Mus musculus; RNA-Seq
the total RNA of tumor tissues in 5% glucose and CM-ZIF8 at Ce6/Lon+L group were  extracted using Trizol reagent. RNA-seq libraries were prepared according to the manufacturer's library construction protocol.RNA quality was evaluated by Agilent 2100 BioAnalyzer (Agilent RNA 6000 Nano Kit). mRNA was purified via an oligo (dT) and subjected to sequencing library constructions. The  library quality  was performed using an Agilent 2100 Bioanalyzer and ABI StepOnePlus Real-Time PCR System. RNA-seq was carried out using an Novaseq600 with 150 base pair reads.