GSM7506561: 02SAM_3 H3K4me3; Homo sapiens; ChIP-Seq
Nuclei of PC-3 cells were disrupted and sonicated to yield fragment size ranging from 200-500bp. Lysates were incubated with H3K4me3 and H3K27me3 antibody (Rabbit polyclonal) and bound DNA after washes was extracted using Qiagen PCR cleanup kit.