ITS amplicon sequencing of dry-aged meat
Total DNA of each sample was extracted from 5 ml of a suspension obtained after homogenization with a stomacher of ~20 g of meat supplemented with a volume of tryptone salt to obtain a 1:10 dilution. After centrifugation at 8000 g for 15 min at 4C and removal of the supernatant, total DNA was extracted from the pellet using the FastDNA SPIN Kit (MP Biomedicals) following the manufacturer's instructions. For the analysis of fungall diversity, the ITS2 region was amplified and then sequenced at the Genome Quebec platform (MacGill University, Montreal, Canada) with Illumina Miseq PE300 technology, generating 2 x 300 bp reads.