Hi-C
Hi-C is a special technique to provide a genome-wide view of the spatial proximity of chromatin loci. Due to chromatin regions located at spatially adjacent sites usually require protein immobilization, DNA-protein complexes are crosslinked with formaldehyde. The cross-linking allows a tight binding of DNA and proteins at spatially adjacent sites followed by the digestion of cross-linked DNA molecules with restriction endonucleases or other DNases. The ends of linear fragments are released, marked with biotin, and ligated. The ligated DNAs in the crosslinked complexes are extracted. Biotin-labelled DNA loci are then captured. General library construction and sequencing are conducted to obtain the loci information of captured sequence at both ends.