RNA-Seq of Homo sapiens: monocyte-derived macrophages
2 g of AMPure XP (Becton Dickinson, Burlington, NC, USA) bead-purified RNA was treated with a Ribo-off rRNA Depletion Kit (Human/Mouse/Rat, Vazyme, Nanjing, Jiangsu, China) to deplete rRNA (including cytoplasmic 28S, 18S, 5S rRNA, and mitochodrial 12S, 5.8S rRNA) from human total RNA (Illumina, San Diego, CA, USA). The sequencing libraries were prepared following the manufacturers instructions for KAPA Stranded RNA-Seq Kit, (KAPA Biosystems, Roche, Basel, Switzerland). RNA isolation, library generation, and RNA sequencing were performed in three independent replicates.