Illumina NovaSeq 6000 paired end sequencing; RNA-seq of C. elegans young adult hermaphrodites to detect differentially expressed genes in worms exposed to the nematicide cyclobutrifluram.
RNA-seq of C. elegans young adult hermaphrodites to detect differentially expressed genes in worms exposed to the nematicide cyclobutrifluram.
Control and worms exposed to the nematicide CB (24h) were collected as young adult hermaphrodites 50-h postlarval hatching after hypochlorite treatment (at 20 ì§¸C) (Stiernagle, T. 2006). Total RNA was extracted with TRIzol Reagent (Invitrogen; Carlsbad CA, USA), and RNA was purified using the PureLink RNA Mini Kit (Invitrogen; Carlsbad CA, USA) according to manufacturer's instructions. cDNA libraries were built using the TruSeq stranded mRNA library preparation kit (Illumina Inc.; San Diego CA, USA) at the Next Generation Sequencing (NGS) Platform in Bern (https://www.ngs.unibe.ch/).