GSM7986525: MOLM13, EGR1, 4C; Homo sapiens; OTHER
Cells were fixed with 2% formaldehyde for 10 minutes at room temperature. After quenching and cell lysis, the nuclei were then treated with SDS followed by the treatment of Triton X-100 solution to sequester the SDS. Next, the nuclei were taken up for digestion with DpnII and ligation. After the first ligation, DNA were reverse crosslinked, purified and subjected for the second digestion with NlaIII and the second ligation. DNA was then purified using phenol–chloroform–IAA solution extraction and ethanol precipitation. Dissolved DNA was further purified with QIAquick PCR Purification Kit (QIAGEN, 28104). Extracted DNA were served as template to perform PCR reactions. PCR products were purified using QIAquick PCR Purification Kit (QIAGEN, 28104) and KAPA DNA beads. The libraries were then sequenced on Illumina platform.