DNA-Seq of Homo Sapiens: Vietnamese colorectal cancer patients
Genomic DNA was isolated from FFPE by the QIAamp DNA FFPE Tissue Kit (Qiagen, USA) according to manufacturers instructions. DNA fragmentation and library preparation were performed using the NEBNext Ultra II FS DNA library prep kit (New England Biolabs, USA). Libraries were hybridized with predesigned probes for a gene panel of 95 targeted genes (Integrated DNA Technologies, USA). DNA libraries were sequenced on the DNBSEQ-G400 sequencer (MGI, China) with an average target coverage of 200X.