GSM7505506: n51_responder_epidermis; Homo sapiens; RNA-Seq
Skin punch biopsy samples were rinsed in sterile phosphate buffered saline without calcium or magnesium (HyClone Laboratories, GE Healthcare) and then incubated at room temperature in a 3.8% ammonium thiocyanate (Sigma Aldrich) in phosphate buffered saline without calcium or magnesium solution for 45 minutes. The epidermis and dermis were subsequently separated using sterile forceps, placed into separate RNase-free tubes containing 700 μL QIAzol (Qiagen), homogenized by hand using RNase-free pestles (Argos), and placed at −80 °C. All samples were collected, processed under sterile conditions, and stored within a 2-hour time frame. RNA was isolated using chloroform (Fisher Scientific), RNeasy Mini Kit (Qiagen), and RNase-free DNaseI (Qiagen). Briefly, 140 μ