foliar endophytic fungi of Ageratina adenophora inoculated by litter and soil,
the amplified products were quantitatively mixed and sequenced on the computer: the purified PCR products were evaluated using the Agilent 2100 biological analyzer (Agilent, USA) and the library quantitative kit of Illumina (Kapa Biosciences, Woburn, MA, USA). The qualified library concentration should be more than 2nM.