GSM6600647: T4-1-methylation; Oryza sativa; Bisulfite-Seq
Flag leaves from the descendant Fix clonal plants (Fix-T1, T2, T3 and T4), Fix-T0 and CY84 at heading time were ground to a fine powder in liquid nitrogen using a mortar and pestle. Genomic DNA was isolated using a Plant Genomic DNA Purification Kit (Tiangen) and sent to the GENEWIZ (Suzhou, China) for bisulfite treatment, library preparation, and next-generation sequencing on an Illumina HiSeq. 2000 platform (Illumina).Flag leaves from the descendant Fix clonal plants (Fix-T1, T2, T3 and T4), Fix-T0, CY84 and four generations Nipponbare variety (1th, 2th, 3th and 4th) at heading time were ground to a fine powder in liquid nitrogen using a mortar and pestle. Total RNA was isolated using TRIzol reagent (Invitrogen, 15596026) and sent to the GENEWIZ (Suzhou, China) for RNA-seq library construction and sequencing on an Illumina HiSeq. 2000 platform. Genomic DNA was isolated using a Plant Genomic DNA Purification Kit (Tiangen) and sent to the GENEWIZ (Suzhou, China) for bisulfite treatment, library preparation, and next-generation sequencing on an Illumina HiSeq. 2000 platform (Illumina).e. Total RNA was isolated using TRIzol reagent (Invitrogen, 15596026) and sent to the GENEWIZ (Suzhou, China) for RNA-seq library construction and sequencing on an Illumina HiSeq. 2000 platform.