GSM7770390: IgG4-RD patient #1; Homo sapiens; RNA-Seq
Human submandibular gland biopsy samples were dissociated by a gentleMACS octo dissociator. Digested submandibular gland cells in cell suspension were separated using Percoll solution. Subsequently, submandibular gland cells were stained with anti-human CD45 PE and non-hematopoietic cells (CD45-) were purified from stained cells using a FACS cell sorting system (BD Aria III) and then encapsulated in droplets. Libraries were then prepared using Chromium Single Cell 3' Reagent Kits v3.1 following the manufacturer's protocol. The generated scRNA-seq libraries were sequenced using a total of 127 cycles (paired-end reads) with a NovaSeq 6000 using NovaSeq 6000 S1 Reagent 100 Cycles Kit.