GSM7587730: MDA-MB-231, No CM, rep 2; Homo sapiens; RNA-Seq
Total RNA was harvested using Rneasy Mini Plus Kit (Qiagen) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis. Subsequently, a series of enzymatic steps were done for end repair, A-Tailing and ligation with NEB adapters, followed by PCR amplification and purification.