RNA-Seq of Allium hookeri roots
The isolated RNA samples were subjected to library preparation using Illumina TruSeq Stranded mRNA Sample Preparation Kit. The oligo-dT based enriched mRNAs were fragmented and converted into first-strand cDNA, followed by second-strand generation, A-tailing, adapter ligation followed by by limited number of PCR amplification of the adaptor-ligated libraries. Library quantification and qualification was performed using DNA High Sensitivity Assay Kit. The amplified library was analyzed on Bioanalyzer 2100 (Agilent Technologies) using High Sensitivity (HS) DNA chip with quantification by Qubit Flourometer. The libraries were pair end sequenced using Illumina platform.