RNA-seq of common carp liver, replicate 3
Total RNA was isolated from 2 samples using TRIZOL (Invitrogen, USA) and  TRIpure total RNA rapid extraction kit ( Biofit, RN03) following manufacturers protocol. 100 ng of total RNA was used for cDNA synthesis using ReverTra Ace qPCR RT Master Mix with gDNA Remover kit (TOYOBO, FSQ-301). The cDNA was then used for Illumina library construction. Adaptors were ligated to the cDNA which had been end-repaired. Each prepared tissue cDNA was sequenced with 150-bp paired-end reads on HiSeq X Ten.