Pore-C of Plecia longiforceps
Twenty male adult specimens were used for Pore-C DNA extractions following the standard procedure (Deshpande et al. 2022) with slight modifications. Pore-C libraries were constructed using the Ligation Sequencing Kit V10 (Oxford Nanopore Technologies (ONT), UK) and sequenced on an ONT PromethION platform at the National Instrumentation Center for Environmental Management (NICEM), Korea. Reads with a phred quality score of 7 or higher were selected using the Guppy 6.5.7 software provided by ONT.