UCE target enrichment of Austrohormius sp. CNIN4020
Genomic DNA was extracted from voucher specimens using a non-destructive technique with the EZ-10 Spin Column Genomic DNA minipreps Kit (Bio Basic, Toronto, Canada) following the manufacturers protocol. DNA extractions were quantified using Qubit 4.0 (Life Technologies). For each sample, up to 100 ng of DNA was fragmented to an average fragment distribution of 400-600 bp using a Bioruptor Pico sonication device. Genomic libraries were constructed following Branstetter et als (2017) protocol using the Kapa Hyper Prep library preparation kit (Kapa Biosystems Inc., Wilmington, MA, USA) and TruSeq-style dual-indexing barcodes (Glenn et al., 2019). Target enrichment was performed using the enhanced commercial myBaits UCE set (Hym v1, ArborBiosciences, Ann Arbor, MI, USA) that were designed for Hymenoptera and developed by Faircloth et al. (2015) and Branstetter et al. (2017). Enriched libraries were pooled at equimolar ratios for sequencing. The sequencing service was conducted in an Illumina HiSeq 2500 instrument (PE125, v4 chemistry) at Admera Health BioPharma Services (New Jersey, USA).