GSM7592614: M-KI4_S12; Mus musculus; RNA-Seq
RNA was then extracted using the Qiagen miRNeasy Mini Kit. RNA quality and quantity were assessed with the RNA 6000 Nano kit on the Agilent 2100 Bioanalyzer. For forebrain heart, muscle and striatum, sequencing libraries were prepared using the Illumina TruSeq Stranded mRNA Library Prep kit, using 1 microgram total RNA as input. For cerebellum and spinal cord, sequencing libraries were prepared using the Illumina Stranded mRNA Library Prep kit Ligation, using 500 nanograms Total RNA as input. Library size was assessed with the DNA 1000 Kit on the Agilent 2100 Bioanalyzer.