No Zinc R3
Aliquots of RNA samples were used for construction of cDNA libraries. cDNAs were amplified, purified and size selected from an agarose gel and sequencing was performed by the GenOne Biotechnologies company (www.GenOne.com.br) using the Illumina HiSeq 2500 platform.  The reference genome of P. brasiliensis was used for the annotation of data from the sequencing through the ENSEMBL program, version 44 (https://www.ensembl.org/index.html) [55]. The reference genome was indexed, and readouts mapped and aligned using STAR software [56]. Readings mapped to more than one locus were deleted [57].