RNA-Seq of siSNRPD2 treated control fibroblast
RNA was isolated from cells using the Aurum Total RNA mini kit (Bio-Rad) with on-column DNase I treatment as outlined in the manufacturers protocol. RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit (Illumina) following rRNA Depletion (NEBNext Kit), with a total of ~ 350 ng of input RNA. The protocol was carried out following the manufacturer's instructions, with the following exceptions: 40 adaptor dilutions; bead incubations were performed at room temperature and 10 cycles of library amplification. Libraries were quantified using the NEBNext Library Quant Kit for Illumina, inspected on the Advanced Analytical Fragment Analyzer using the NGS Analysis DNF-474 kit, pooled at equimolar concentrations and sequenced on the Illumina NextSeq 2000 sequencer