GSM8242958: PR_rep3; Mus musculus; RNA-Seq
For comparison of PR and CR tumor clones, RNA was isolated from in vitro cell cultures in triplicate using the RNeasy plus mini kit (QIAgen). For in vivo comparison of CAR-T cells and tumors, mice were engrafted with CR or GPR65 KO tumors and treated with CAR-T cells 7 days later. Spleens were harvested 4 days after CAR-T treatment and RFP+ tumor cells and Thy1.1+ CAR-T cells were isolated by FACS. Tumor cells from untreated mice were isolated after 7 days of tumor engraftment. RNA was isolated from sorted cells and 3 biological replicates were submitted for RNA-sequencing For total RNA-seq, library was constructed from RNA using Illumina TrueSeq stranded mRNA library prep kit and sequenced using the HiSeq or NovaSeq platforms (2 × 101-bp paired-end reads)