GSM6402609: Frontal-Cortex_Psilocybin_3-hours-post-injection_4; Mus musculus; RNA-Seq
Total RNA was isolated using an RNeasy mini kit (Qiagen, #74106) according to the manufacturer's protocol Messenger RNA sequencing libraries were prepared using the Illumina TruSeq Stranded mRNA protocol (Illumina). Poly-A containing mRNAs were purified by poly-T attached magnetic beads, fragmented, and cDNA was synthesized using SuperScript III Reverse Transcriptase (Thermo Fisher Scientific). cDNA was adenylated to prime for adapter ligation and after a clean-up using AMPure 