GSM8105621: 2-BM-H2BHiMMPLo [JB1_6]; Mus musculus; RNA-Seq
Murine bone marrow c-Kit cells were isolated for droplet based single-cell RNA-Seq (scRNA-Seq) analysis. All captures were performed using the Chromium 3' version 3.1 kit (10X Genomics). Emulsion, Gel Beads-in-emulsion (GEM) collection, clean-up, and cDNA amplification were performed according to 10X Genomics protocols. All libraries were constructed with single index and sequenced on multiple Illumina S4 flow cells with PE150+10+10 setting (Illumina). Paired-end sequencing using an Illumina NovaSeq 6000 SP Flow Cell was performed, at a target depth of 50 million paired-end reads.