Stem tissue from Vitis amurensis: Plant B, Summer
The DNA was extracted from 30 mg of V. amurensis tissue using the ZymoBIOMICS DNA miniprep kit per manufacturers protocol (Zymo Research, USA). DNA was assessed for quality and quantity using the NanoPhotometer P300 (IMPLEN, USA).  DNA samples were sent to Evrogene (Moscow, Russia) for high-throughput sequencing using Illumina technology. Libraries were prepared for sequencing according to the protocol described in the manual "16S Meta-genomic Sequencing Library Preparation" (Part # 15044223 Rev. B; Illumina). Bacterial 16S rRNA regions were amplified from all samples using the modified for Vitis sp. plants primers 515F (5GGTAATACGKAGGKKGCDAGC) and 806R (5RTGGACTACCAGGGTATCTAA). After obtaining the amplicons, the libraries were purified and mixed equimolarly using the SequalPrep Normalization Plate Kit (ThermoFisher, Cat # A10510-01). Quality control of the obtained library pools was performed using Fragment Analyzer and quantitative analysis was performed using qPCR. The library pool was sequenced on Illumina MiSeq (2  250 paired end) using MiSeq Reagent Kit v2 (500 cycles). The FASTQ files were obtained using bcl2fastq v2.17.1.14 Conversion Software (Illumina). The phage PhiX library was used to control sequencing parameters. Most of the reads pertaining to phage DNA were removed during demultiplexing.