GSM8191280: human venous blood astnmatic 1; Homo sapiens; miRNA-Seq
2.5ml of children's venous blood was drawn from fasting subjects using a PAX-gene Blood RNA tube(BD Company,USA).RNA was harvested using PAXgene Blood miRNA Kit(PreAnalytiX, Switzerland),RNA sample concentration ≥ 100 ng/µL, total amount ≥ 2μg, OD260/280 value between 1.8 and 2.2, OD260/230 ≥ 2.0, and RIN ≥ 7 detected by Agilent 2100 Bioanalyzer. High-resolution polyacrylamide gel electrophoresis (PAGE) was used to isolate miRNA libraries with insert fragment sizes of 22-24 NT