GSM8147465: hiPSC-differentiated Schwann cells replicate 1; Homo sapiens; RNA-Seq
RNA was isolated from hESC- and iPSC-derived Schwann cells at passage two with TRIzol (Thermo Fisher Scientific) following the manufacturer's protocol and subsequent purification with a RNeasy Mini Kit (Qiagen). RNA quality was verified with a bioanalyzer. Library was prepared using Illumina TruSeq Stranded RNA (100 ng). RNA-sequencing was carried out using TrueSeq RiboZero gold (stranded) kit (Illumina).