GSM8061339: LuCaP70CR, Hi-C; Homo sapiens; Hi-C
FOXA2-D14, D21 and D28 LNCaP cells, NCI-H660 cells and the cells from LuCaP PDXs were fixed with 2% formaldehyde (final concentration) at room temperature for 10 min, followed by adding 2.5 M glycine to a final concentration of 0.2 M to quench the reaction. Hi-C libraries were generated using the Arima-HiC+ kit (Arima Genomics, A510008) for cell line and PDXs, Arima-HiC+ FFPE Kit (Arima Genomics, A101060) for CRPC and NEPC FFPE samples. The Hi-C libraries were sequenced with paired-end 2 × 150 bp using Illumina NovaSeq6000.