Whole Blood RNAseq
The total RNA was obtained using the MagMax for Stabilized Blood Tubes RNA Isolation Kit (ThermoFisher). The TruSeq Stranded Total RNA Library Prep kit with RiboZero Gold (Illumina, San Diego, California, USA) was used to prepare the total RNA libraries with an input of 500ng. Then, the libraries were loaded onto NextSeq 500/550 High Output Kit v2.5 (150 Cycles).