GSM6600398: AML2_sgLuc_DMSO_Rep1; Homo sapiens; RNA-Seq
Total RNA was isolated using RNeasy kit (Qiagen, Germany). RNA-seq libraries were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs), following the manufacturer's protocol. The quality and concertation of the libraries were examined by Bioanalyzer High Sensitivity DNA Chip (Agilent). The multiplexed RNA-Seq libraries were paired-end sequenced for 150 bp on the Illumina HiSeq 4000 platform.