GSM8012184: FACS sorted tumor cells from AZD1390 treated CPC tumors biological replicate 2; Mus musculus; RNA-Seq
Sorted cells were submitted for 10X sequencing and libraries were prepared using the Chromium Single Cell 3' Reagents kits v.3. Samples were resuspended in PBS with 0.04% BSA and loaded onto the Chromium microfluidic chip (10x genomics) to generate single-cell bead emulsions with individual 10x barcodes. RNA from barcoded cells was reverse transcribed in a C1000 Touch Thermal Cycler (Bio-Rad) and libraries were prepared according to manufacturers protocols (12 cycles for cDNA amplification). cDNA quantity and quality was measured using an Agilent TapeStation 4200 and about 375ng of material was used for library preparation. Libraries were normalized to equal molar concentrations (10nM) and pooled before being sequenced on a NovaSeq 6000.