GSM7099105: Sample2_D21_VDJ; Mus musculus; RNA-Seq
2x10^4 probe+ CD19+ Ighg2A10 B cells were transferred into recipient mice. 1-2 days later mice were immunized IV with 5x10^4 irradiated Pb-PfCSP sporozoites. At either 7 or 21 days post immunization mice were euthanized, spleens collected and probe+ IgD- Ighg2A10 B cells stained with TotalSeq-C feature and hashtag antibodies were isolated and sorted. Cells were then loaded into a Chromium (10x Genomics). Indexed V(D)J, Feature Barcode and GEX libraries of sorted samples were prepared according to the protocol for Single Indexed 10X Genomics V(D)J 5' kit for mice with Feature barcoding kit (10X Genomics).Libraries were then sequenced using an Illumina NovaSeq6000.