RNA-seq of WT with H2O2 threatment
The enriched mRNA was fragmented into approximately 200 nt lengths. The fragments were used for first-strand cDNA synthesis, and then second strand cDNA synthesis was performed. The double-stranded cDNA was subjected to reactions to perform end-repair, dA-tailing, and adaptor ligation. The modified fragments were isolated using Agencourt AMPure XP beads, and enriched by PCR amplification. Finally, the cDNA libraries were sequenced on a flow cell using an Illumina HiSeq? 2500 sequencing platform