GSM7729940: S57; Mus musculus; RNA-Seq
Mouse kidneys was perfused, excised, minced, digested to obtain single cell suspension as described before. Cells were collected by centrifugation, subjected to live-death dye and sorted by flow cytometry using BD FACS ARIA system. Isolated living single cell suspensions were converted to barcoded scRNA-seq libraries by using the Chromium Single Cell 3′ Library, Gel Bead & Multiplex Kit, and Chip Kit V3, loading an estimated 7,000–12,000 cells per library/well, and following the manufacturer's instructions. Indexed libraries were sequenced using Illumina NovaSeq 6000 platform, where 150bp pair-end sequences were obtained.