GSM6796866: Pig RCA saline_C; Sus scrofa; RNA-Seq
ST was conducted by following Visium/10x genomics pipeline. RNA extraction step was performed using Visium Spatial Gene Expression Slide & Reagents Kit, (4 rxns, cat# 1000187). Second strain synthesis & denaturation, cDNA amplification and cleanup, cDNA quantification and QC were performed using reagents from Visium Spatial Gene Expression Slide & Reagents Kit (cat# 1000187). CDNA library was constructed with Visium library construction kit (cat#1000190). Library quantification was performed with KAPA library quantification kit (Roche, KR0405). dual index (i5/i7) RNA-seq