RNA-Seq of Pseudomonas sp. NY1
The TruSeqTM Stranded Total RNA Library Prep Kit reagent was used to construct the library. DUTP was used instead of dTTP in the dNTPs reagent for the synthesis of the second chain of cDNA, so that the base of the second chain of cDNA contained A/U/C/G. Before PCR amplification, the second strand of cDNA was digested with UNG enzyme so that only the first strand of cDNA was included in the library.