DNA-seq of Malus Domestica: Meristematic layer 1,2 of upper mutant branch of RubyMac
Leaf surface tissue (L12) was isolated from leaf petioles by scraping the petiole with a razor blade to remove the outer tissue layers and directly drop them into liquid nitrogen and stored at -80 C until use. L12 tissue was ground in liquid nitrogen in a mortar and pestle prior to extraction. DNA was extracted using the DNeasy Plant Mini Kit (Qiagen, Hilden, Germany). DNA samples were sequenced at The Max Planck Genome Center Cologne.