GSM6600420: PARAIL GapmeR 1, Rep2; Homo sapiens; RNA-Seq
Total RNA was isolated from CD14+ monocytes and THP1 macrophages using RNeasy mini kit and on-column DNase I digestion to eliminate genomic DNA (Qiagen). RNA-Seq. Libraries were prepared according to instructions accompanying the Illumina® Illumina Stranded Total RNA Prep with Ribo-Zero kit. Libraries were sequenced on the Illumina HiSeq 2500 following the manufacturer's protocols (CD14+ mnocytes).  For THP1 cells Libraries were prepared using KAPA mRNA HyperPrep with PolyA protocol.