RNA seq of MDAMB231 Parental control cells
The paired-end sequencing library was prepared using NEBNext Ultra RNA Library Prep Kit for Illumina.(NEB #E7770) The library preparation process was initiated with 1000 ng input. mRNA enrichment was performed as per user manual and mRNA was subjected to fragmentation, first & second-strand cDNA synthesis, end-repair, 3 adenylation, adapter ligation, selective enrichment of adapter-ligated DNA fragments through PCR amplification, followed by validation of Library on Agilent 4150 tape station. The final library was pooled with other samples, denatured & loaded on to flow cell. On the flowcell, cluster generation & sequencing was performed using Illumina Novaseq 6000 platform to generate 2150bp paired-end (PE) reads.