The complete mitogenome of Menippe rumphii (Decapoda: Brachyura: Eriphioidea) and phylogenetic relationships within Infraorder Brachyura
After the sample genome DNA is tested to be qualified, break the DNA with enzyme, and then purify the broken DNA to build a sequencing library. The steps are as follows: repair the DNA end, add A to the 3 'end, connect the sequencing connector, recover the target segment by agarose gel electrophoresis, and conduct PCR amplification on the target segment to finally build a sequencing library. Before sequencing, the constructed library is subjected to quality inspection. After passing the inspection, Illumina Novaseq TM platform is used for sequencing