Cell_suspension
Total RNA was extracted separately from 100 mg of CS, EG, LG, and WO tissues in two biological replicates using the RNeasy Plant Mini Kit (cat. No. 74104, Qiagen). The RNA samples were evaluated for concentration using the Shimadzu BioSpec-nano instrument (Shimatzu), and their integrity was assessed through agarose gel electrophoresis. Novogene (USA) conducted library preparation and sequencing using the Illumina Novaseq 6000 platform.