GSM8012956: BMDMs, Control 3; Mus musculus; ATAC-seq
To get BMDMs, bone marrow cells isolated from C57/BL6 mice were cultured  in RPMI 1640 differentiation medium containing 10% fetal bovine serum, 1 mM sodium pyruvate, antibiotics, and 30% L929 supernatant for 7 days. BMDMs were harvested and replated in 20% L929 medium one day before treatments. All cells were cultured in humidified atmosphere with 5% CO2 at 37 °C ATAC-seq library preparation were performed by Mayo Clinic Epigenomic Development Laboratory and Recharge Center