Bacterial 16S metagenom of water from 3  km away from mouth of channel Srednyaya (7 m)
The V2-V3 variable region of the bacterial 16S rRNA gene was amplified by PCR with primers, containing rRNA-specific parts: B_V23F (5-AGTGGCGGACGGGTGAGTAA) and B_V23R (5-CCGCGGCTGCTGGCAC). The libraries were analyzed using Illumina MiSeq Standard Kit v.3 (Illumina) in the Shared Access Center "Genomic technologies, proteomics, and cell biology" of the All-Russia Research Institute for Agricultural Microbiology (Pushkin).