GSM8285585: PB-ON; Homo sapiens; RNA-Seq
Total RNAs were extracted using Trizol. For the enrichment of biotinylated RNAs, total RNAs were mixed with 20μL of Dynabeads MyOne Streptavidin Beads C1, rotating overnight at 4°C, and eluted as previously described. Eluted RNAs were purified using GeneJet RNA Concentration Kits (ThermoFisher). RNA libraries were prepared for sequencing using NEBNext UltraTM RNA Library Prep Kit