GSM8287230: ZHBTc4, LIF, Dox, 48h, Input; Mus musculus; ChIP-Seq
Cells were crosslinked with formaldehyde and subsequently sonicated. Sonicated cell extracts were used for ChIP assays. ChIP-enriched DNA was end-repaired using the NEBNext Ultra II End Repair/dA-Tailing Module, and ligation of adapters (Illumina) or custom indexed adapters. PCR was performed using Phusion High Fidelity PCR master mix