GSM7750269: CAMA-1 ATAC-Seq, biol rep1; Homo sapiens; ATAC-seq
Two biological replicates of 50,000 CAMA-1 cells were lysed to prepare nuclei for the transposition reaction. A Tn5 enzyme was used for the transposition reaction and samples were cleaned up with the Qiagen MinElute PCR Purification Kit (​​CAT #28004) with 250 μL of buffer PB for each 50 μL sample. The libraries were amplified for 7-8 PCR cycles. The libraries were then cleaned-up with the Qiagen MinElute PCR Purification Kit and size selected with Beckman Coulter AMPure beads. Library quality control was performed by ensuring greater than ten fold enrichment for open chromatin with a qPCR for two constitutively open (GAPDH and KAT6B) and closed regions (QML93 and SLC22A3) . Further library quality control was performed with a Qubit High Sensitivity dsDNA kit and Agilent 2100 Bioanalyzer.