GSM8062040: PerC, B2, KO, 1; Mus musculus; RNA-Seq
Sort-purified peritoneal B-1a, B-1b, and B-2 cells obtained fromTET2KO andTET2WT C57BL/6 mice were RNA and DNA extracted by using the Qiagen AllPrep kit. The purified RNAs were stored at −80 °C and sent to Novogene to perform ultra-low input bulk RNA sequencing. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library.  For the non-directional library, it was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.