GSM7911760: GM12878_sgWAPL_3; Homo sapiens; RNA-Seq
Total RNA was isolated using a Qiagen RNeasy extraction kit. An on-column DNA digestion was performed to remove any genomic DNA contamination. For library preparation, 1 µg of RNA was used for each sample, polyA-enrichment was performed using the NEBNext Poly(A) mRNA Magnetic Isolation Module (#E7490, New England Biolabs), and library construction was completed using NEBNext Ultra RNA Library Prep Kit for Illumina (#E7760L, New England Biolabs) as per the manufacturer's instructions.