GSM8244979: Chow-Saline-2; Mus musculus; RNA-Seq
Single cell RNA-sequencing of lung and eWAT immune cells was performed on CD45+ immune cells using FACS. Sorted cells from two independent biological replicates were pooled and assessed for viability with a live dead stain (Zombie - UV). Approximately 10,000 cells were loaded into separate ports of a 10X Genomics Chromium 3′ version 3.1 kit. Emulsion, Gel Bead-In Emulsions (GEM) collection, clean-up and cDNA amplification were performed according to 10x Genomics protocols. Library preparation was performed according to manufacturer instructions. The 10x Genomics libraries were sequenced on Illumina S4 flow cells with PE150 +10 +10 setting (Illumina).  BCL files were demultiplexed into FASTQ files for CellRanger input.