GSM7596367: MYLA, romidepsin + afatinib dimaleate, rep 3; Homo sapiens; RNA-Seq
Total RNA was extracted from CTCL cell lines treated with single drugs and combination using the RNeasy kit (Qiagen, Hilden, Germany) following manufacturer's protocol. RNA quality and concentration was determined using a Nanodrop 2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA). RNAseq libraries were prepared from total RNA isolated from treated CTCL cell lines and sequenced by Azenta Life Sciences (South Plainsfield, New Jersey, USA).