MinION sequencing; Long read sequencing of isolate FF10_0221
Extraction of genomic DNA was done using the Quick-DNA HMW MagBead Kit (D6060, Zymo) and the QIAamp DNA Mini Kit (51306, Qiagen). DNA was assessed for quantity using a Qubit 4.0 fluorometer and quality using a Nanodrop spectrophotometer. The input concentration was adjusted to approximately 53 ng/mL. Library preparation was done using the Rapid Barcoding Sequencing kit (SQK-RBK004, Oxford Nanopore Technologies).