GSM7507087: Col-0_mRNA_rep1; Arabidopsis thaliana; RNA-Seq
Total RNA was purified from 2-week-old seedlings using TRIzol. mRNAs were enriched using VAHTS mRNA Capture Beads (Vazyme, N401) Enriched RNAs were fragmented to 200-300 bp by incubating with 1 × Frag/Prime Buffer for 5 min at 94°C. Fragmented RNAs were used for first strand synthesis, followed by second strand synthesis. Double stranded cDNAs were end-repaired and ligated to adapters. Ligated products were purified and sorted with VAHTS DNA Clean Beads (Vazyme, N411). Purified products were amplified for 12-15 cycles and the PCR products were cleaned by Agencourt AMPure XP beads (Beckman Coulter, A63881). Samples were barcoded and subjected to Illumina sequencing by Annoroad Biotech (Beijing).