GSM7679533: SNU719 H3K27ac HiChIP rep2; Homo sapiens; Human gammaherpesvirus 4; OTHER
25 million cells were fixed with 1% formaldehyde for 10 min and then quenched with 0.125 M glycine for 5 min at RT. Ligation products were immunoprecipitated with 25 µg H3K27ac (Active Motif 39133). 5 μL of Streptavidin C-1 beads were used for biotin pull-down. After the last wash with Tween Wash Buffer bead-bound DNA was used to prepare libraries with Ultralow Library Systems V2 (Tecan Genomics, part no. 0344NB-32) as per manufacturer's protocol