GSM6407493: RCMB20; Homo sapiens; ATAC-seq
Sorted cells were spun at 400 g for 5 min at  4°C. Cell pellets were resuspended in 50 ul of cold lysis buffer (10 mM Tris-HCl, pH 7.5, 10 mM NaCl, 3mM MgCl2, 0.1% NP-40, 0.1% Tween-20 and 0.01% Digitonin) and incubated on ice for 3 min. 1 mL of wash buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 3mM MgCl2, and 0.1% Tween-20) was added to the suspension and was then spun at 500 g for 10 min at 4°C. The nuclei were then resuspended in the transposition reaction mix, using the Illumina Tagment DNA Enzyme and Buffer Kit (Illumina, 20034197) (25 ul of 2X TD buffer, 5 ul of TDE1 tagment DNA enzyme, 16.5 ul of 1X PBS, 0.1% Tween-20, 0.01% Digitonin and 5ul of nuclease free water). The suspension was incubated for 30 min at 37°C on thermomixer at 1,000 rpm. DNA was then isolated using the Qiagen MinElute kit (Qiagen, 28204).