GSM8111651: zip2fed3; Caenorhabditis elegans; RNA-Seq
For total RNA extraction, L4 worms were thawed in Trizol and sonicated. Total RNA was extracted from the mixture using a Trizol and 1-bromo-3-chloropropane extraction, as described (PMID 35285794). RNA integrity and quantity were assessed on an Agilent Technologies 2100 Bioanalyzer System. Construction of strand-specific mRNA sequencing libraries and sequencing (75bp PET) on an Illumina HiSeq 2500 machine was done at the Sequencing Services facility of the Genome Sciences Centre, BC Cancer Agency, Vancouver BC, Canada.