GSM7881807: Mki67-/- Small pre-B 4; Mus musculus; ATAC-seq
Cells were pelleted at 500xg for 10 minutes at 4 degree. Tagmentation reaction containing 0.02% Digtonin, 0.1% Tween-20, Tagment DNA Enzyme 1, Tagment DNA Buffer and moleular grade H2O in 25 uL volume per sample was added to each of the cell pellet. Tagmentation was performed at 37 degrees for 60min followedd by 30 min digestion with Proteinase-K DNA samples were first purified by SPRI bead size selection. Libraries were amplified using Kapa HiFi HotStart Polymerase Ready Mix in 50 uL volume with indexing primers. A post-PCR SPRI bead clean up was performed to purify the libraries.