GSM7750358: ATE_AO_I; Acropora tenuis; RNA-Seq
For RNA isolation, due to the sheer size, samples collected from multiple batches were combined to acquire an adequate amount of RNA for sequencing. Total RNA was isolated using the TRI Reagent® according to the manufacturer's protocol. RNA quality was assessed using Agilent RNA 6000 Nano Kit on Agilent 2100 Bioanalyzer (Agilent, USA), and samples with RNA integrity number ≥ 8.0 were used for sequencing. The CORALL RNA-Seq Library Prep Kit (Lexogen GmbH) was used for library preparation. Before sequencing, the libraries were pre-assessed by Agilent High Sensitivity DNA Kit (Agilent, USA) and quantified using Qubit™ 1X dsDNA HS Assay Kit (Invitrogen™). The sequencing was outsourced (GENEWIZ Illumina NovaSeq™ 2x150 bp sequencing), generating 15 million paired-end reads per replicate.