GSM8245453: HN_BL_189_human_invitroTh2_PI_4h_donor1; Homo sapiens; RNA-Seq
Approximately 20,000–50,000 cells (cell viability >98%) were lysed with TRIzol (Life Technologies) and total RNAs were extracted using Direct-zol™ RNA MicroPrep (Zymo Research). Total RNAs were subsequently processed to generate an mRNA-seq library using a NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490S), NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, E7530S) and NEBNext Multiplex Oligos for Illumina (Index Primers Set 1) (NEB, E7335S) according to the manufacturer's instructions.