GSM6797137: Hem_WT_1; Mus musculus; RNA-Seq
For each dataset, a region spanning the dorsal pallial midline was dissected on both hemispheres and dissociated using the Neural Tissue Dissociation Kit (P) (Miltenyi Biotec) on the gentleMACS Octo Dissociator following the manufacturer's instructions. Remaining clumps of cells and debris were removed via two rounds of centrifugation for 3 min at 200 g followed by filtration through 30 µm cell strainers (Miltenyi Biotec). To ensure all remaining cells aggregates were dissociated, we finally pipetted the suspension through Gel Saver tips (QSP) and proceeded to a last filtration through a 10 µm cell strainer (pluriSelect). Cell viability and concentration were assessed using a MACSQuant flow cytometer (Miltenyi Biotec). Approximatively 20000 cells were used as input on the 10X Genomics Chromium Controller for a targeted cell recovery of 10000 cells per library. The entire procedure, from the sacrifice of the pregnant female until loading the controller, was achieved in 2h. Single Cell 3′ Library and Gel Bead Kit v3