BGISEQ-500 paired end sequencing of SAMD00436852
"Magnetic beads with Oligo (dT) were used to isolate mRNA. After mixing with the fragmentation buffer, the mRNA was fragmented into short fragments. Then cDNA was synthesized using the mRNA fragments as templates. Short DNA fragments were purified and resolved with elution buffer for end reparation and single nucleotide A (adenine) addition. Subsequently, the short DNA fragments were ligated with adapters. The suitable fragments are selected for the PCR amplification as templates. During the QC steps, Agilent 2100 Bioanalyzer and ABI StepOnePlus Real-Time PCR System were used in the quantification and qualification of the sample library."