GSM7028126: Skin, susceptible, after infestation, bovine 18; Bos taurus; RNA-Seq
Skin biopsies were mechanically crushed with 7 mm metallic beads in the TissueLyser equipment (Qiagen), and total RNA harvested using the RNeasy Fibrous Tissue kit (Qiagen) Sequencing libraries were prepared individually using TruSeq RNA Sample Preparation kit v2 guide (Illumina, San Diego, CA) with standard protocols, clustered using TruSeq PE Cluster Kit v3-cBot-HS kit (Illumina, San Diego, CA) Sequencing libraries were sequenced in a paired-end mode (2 x 100 bp) on a HiSeq1500 equipment (Illumina, San Diego, CA)