GSM7429816: 5_PRERT; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol RNA Isolation Reagent (Life Technologies) from the samples. The quantity and quality of the total RNA were evaluated using an Agilent 2100 bioanalyzer RNA kit. The isolated total RNA was processed for preparation of an RNA-seq library using an Illumina TruSeq Stranded mRNA Sample Preparation kit according to the manufacturer's protocol.