GSM7507170: 1663-PBL-Pop5; Homo sapiens; OTHER
Sorted ASC populations (Pop 2, Pop 3, Pop 4 and Pop 5) from active SLE patients were used to extract the total cellular RNA by using the RNeasy Mini Kit (Qiagen, Inc. Valencia, CA) according to the manufacturer's protocol. Approximately 400 pg of RNA was subjected to reverse transcription using the iScript RT kit (BioRad, Inc., Hercules, CA). Resulting cDNA products were combined with 50 nM VH1- VH6 specific primers and 250 nM Ca, Cm, and Cg specific primers in a 25 μl PCR reaction using High Fidelity Platinum PCR Supermix (Life Technologies, Carlsbad, CA).