GSM6523566: C57BL/6J, PPARαKO, Liver, PFOA0.3, Biol rep 1; Mus musculus; RNA-Seq
Total RNA from liver was obtained by homogenization using TRIzol reagent, followed by isolation and purification by Rneasy mini kit (Qiagen) according to manufacturer's instructions. RNA concentration was measured with a Nanodrop 1000 spectrometer and RNA integrity was determined using an Agilent 2100 Bioanalyzer with RNA 6000 microchips (Agilent Technologies). Library construction and RNA sequencing on BGISEQ-500 were conducted at Beijing Genomics Institute (BGI) for pair-end 100bp runs. At BGI, genomic DNA was removed with two digestions using Amplification grade DNAse I (Invitrogen, USA). The RNA was sheared and reverse transcribed using random primers to obtain cDNA, which was used for library construction. The library quality was determined by using Bioanalyzer 2100 (Agilent Technologies). Then, the library was used for sequencing with the sequencing platform BGISEQ-500 (BGI).