GSM7142657: C. capitata, 1x dose, 12 hpi, rep 1; Ceratitis capitata; RNA-Seq
Each fly was collected in 300 µL TRIsure (Bioline), a guanidine thiocyanate and phenol mixture, and immediately bead homogenized prior to storage at -80℃ . Total RNA was isolated from homogenized samples using the Zymo Direct-zol-96 RNA Purification Kit performed on a KingFisher Flex instrument, which included a mid-protocol DNase I (6U/µL, 5µL) treatment, as well as a secondary Zymo DNase I treatment following RNA isolation. Final RNA was eluted in 20 µL nuclease-free water. RNA quality was assessed using a 4200 TapeStation System with RNA ScreenTape (Agilent), and 3 of the 6 replicates with highest quality for each treatment were selected to constitute the final 81 samples submitted for sequencing. Messenger RNA library was formed by polyA capture (or rRNA removal) and reverse transcription of cDNA, performed by Novogene Corporation Inc.