GSM8111773: s_ovotestis_WT_S7_Av007Ovo_r1.SE; Arion vulgaris; ncRNA-Seq
Total RNA was extracted from samples through phenol-chlorofom isolation, by using QIAzol Lysis Reagent according to the manufacturer´s protocol. Total RNA isolated from tissue as described above was used for NextFlex Small RNA-seq v3 kit (Amplicon). Libraries were prepared according to the manufacturer´s protocol with 3´ adapter ligation overnight at 16 °C, 15 cycles used for PCR amplification and gel purification was performed for size selection.