GSM7678356: control_H3K27ac; Mus musculus; ChIP-Seq
Brain tissue sample were fixed in 1% formaldehyde for 10 minutes at room temperature. After quenching and cell lysis, chromatin was sonicated by Bioruptor Pico (Diagenode, Germany) ultrasonicator. NEBNext Ultra DNA Library Prep Kit was uesed for ChIP-seq library construction.10ng of DNA was ussed as starting material for Input and IP samples. Libraries were validated by the 2100 Bioanalyzer High Sensitivity DNA chip.