GSM8108168: Y_24; Homo sapiens; OTHER
Total RNAs were extracted from samples using TRIzol (Invitrogen™, Cat. No. 15596018) following the methods by Chomczynski et al. DNA digestion was carried out after RNA extraction by DNaseI (Thermo Scientific™, Cat. No.EN0521) About 2μg total RNA of each sample were used for BCR sequencing library preparation using KC-DigitalTM Stranded BCR-seq Library Prep Kit following the manufacturer's instruction. The kit eliminates duplicationbias in PCR and sequencing steps, by using unique molecular identifier (UMI) of 8 random bases to label the pre-amplified cDNA molecules.The library products corresponding to 250-500 bp were enriched, quantified and finally sequenced on DNBSEQ-T7 sequencer