GSM7849941: BMAL1KO_snRNASeq; Mus musculus; RNA-Seq
Fresh gonadal WAT was minced in nucleus preparation buffer (NPB; 10 mM HEPES, 1.5 mM MgCl2, 10 mM KCl, 250 mM sucrose, 0.001% NP-40) with 0.2 U/ul Roche Protector RNase inhibitor followed by dounce homogenization. The homogenate was filtered through a 70 mM cell strainer and centrifuged at 1000 xg for 10 minutes at 4°C, then the nuclear pellet was washed once in NPB followed by resuspension in PBS with 1% BSA and 0.2 U/ul Roche Protector RNase inhibitor. Single-nuclei RNA-seq libraries were prepared using Chromium Next GEM Single Cell 3' Reagent Kits v.3.1 (10x Genomics) according to the manufacturer's instructions.