GSM8111501: OAA1; Homo sapiens; ATAC-seq
The nucleus was collected from 5 × 10^4 cells (plated in a 12-well plate that the density reached 80% with a good growth state) and treated by transposase reaction mix (22.5 μL of nuclease-free water, 25 μL 2 × TD buffer, and 2.5 μL Transposase (Illumina)) at 37 °C for 30 minutes. Transposition and high-throughput DNA sequencing library were carried out by TruePrep DNA Library Prep Kit V2 for Illumina kit. The library products were enriched, quantified and finally sequenced on a Novaseq 6000 sequencer (Illumina) with PE150 model.