GSM7061278: s856; Mus musculus; RNA-Seq
Total RNA was isolated from mPFC punches using Rneasy mini kits from Qiagen (74106) The RNA seq library was generated using NEBNext Ultra II Directional RNAlibrary Prep Kit for Illumina (New England BioLABS, iPSWICH, MS, USA) Sequencing library was validated on the Aligent TapeStation and quantified by using Qubit 2.0 Flurometer as well as quantitative PCR. Libraries were subsequently multiplexed and sequenced on Illumina HiSeq 4000 using a 2x150bp paired-end configuration.