GSM7025953: FC_Droplet_Paired-Tag_H3K27me3_rep1_DNA; Mus musculus; OTHER
Single-cell suspensions were prepared from frozen tissues by douncing in douncing buffer (0.25 M sucrose (Sigma, S7903), 25 mM KCl (Sigma, P9333), 5 mM MgCl2, 10 mM Tris-HCl pH 7.4, 1 mM DTT (Sigma, D9779), 1× Protease Inhibitor, 0.5 U µl−1 RNase OUT, 0.5 U µl−1 SUPERase Inhibitor). The cell suspension was then filtered through a 30-µm Cell-Tric (Sysmex) for debris removal, and spun down for 10 min at 300g, 4 