GSM7679442: pancreatic rat islets, CD_5, 22-day-old; Rattus norvegicus; ncRNA-Seq
Total RNA from rat islets was extracted using miRNeasy micro kit (Qiagen # 217084). Nucleotide modifications were removed from RNA samples prior to sequencing library construction by the following treatments using the rtStar™ tRF&tiRNA Pretreatment kit (Arraystar # AS-FS-005): 3'-aminoacyl (charged) deacylation to 3'-OH for 3' adaptor ligation, 3'-cP (2',3'-cyclic phosphate) removal to 3'-OH for 3' adaptor ligation, 5'-OH (hydroxyl group) phosphorylation to 5'-P for 5' adaptor ligation, and N1-methyladenosine (m1A), 3-methylcytidine (m3C) and 1-methyl guanosine (m1G) demethylation. 10 ng of total RNA was used for the construction of sequencing cDNA libraries using QIAseq miRNA NGS 48 Index IL kit (Qiagen # 331595) following manufacturers's protocol.