GSM7882065: RH4_DMSO_6h; Homo sapiens; ChIP-Seq
For ChIP-seq, the NEB MNase (Catalogue #M0247S) was used. Briefly, cells were fixed with DSG and 37% formaldehyde, treated with MNase, and immunoprecipitated with anti-RNA Pol2 antibody (Santa Cruz Biotechnology, Mouse mAb #sc-47701 X) or anti-H3K27ac antibody (Millipore, Rabbit mAb #MABE647  RM172). This was followed by reversal of crosslinks, proteinase K treatment, and DNA purification. Drosophile spike-in was added to the samples for normalization. Standard Illumina barcodes were introduced during library preparation.