GSM7680190: WT_IgG_forATRX_R4; Mus musculus; OTHER
For histone marks, cells were collected by trypsin. Washed by PBS. For ATRX, cells were gently fixed using 0.1% PFA in PBS for 1 min at room temperature, followed by nuclei extraction with NE buffer (20 mM HEPES (pH7.9), 10 mM KCl, 0.1% Triton X-100, 20% Glycerol, 1 mM MnCl2, 1×cOmplete Mini-Tablet (1 tablet), 0.5 mM Spermidine) according to the protocol from EpiCypher. Libraries were generated using NEBNext Ultra II kits.