GSM6467129: hF27_SPDEFKO, 2; Mus musculus; RNA-Seq
total RNA from fresh frozen tumor chuncks was extracted using the TRIzol Plus RNA Purification Kit (ThermoFisher) per manufacturer's instructions. RNA samples were treated on column with PureLink DNase (ThermoFisher). The quality of purified RNA samples was determined using a Bioanalyzer 2100 (Agilent) with an RNA 6000 Nano Kit. Libraries for RNA-Seq were prepared with the TruSeq Stranded Total RNA Library Prep Human/Mouse/Rat (Illumina) following manufacturer instructions.