GSM6407719: Con_spinal cord-replicate 2; Mus musculus; RNA-Seq
The total RNA of spinal cord tissue was extracted through the Trizol method. Using ribosomal-depleted RNA and random hexamers to synthesize one-stranded cDNA, and then we added buffer, deoxynucleoside triphosphates, and enzymes to synthesize two-stranded cDNA. Double-stranded DNA was purified using VAHTS™ DNA Clean Beads, followed by end repair, A-tailing, and the ligation of sequencing adapters. The cDNA strand containing U was degraded by the uracil-DNA glycosylase enzyme, and finally, PCR enrichment was performed; the PCR product was purified with VAHTSTM DNA Clean Beads to obtain the final strand-specific cDNA library.