GSM6601482: spliceLongMPRE_IP_rep1; Homo sapiens; RIP-Seq
RNA was extracted with NucleoZOL (Macherey-Nagel) RNA was poly-A selected with oligo dT-beads (Dynabeads® mRNA DIRECT™ Kit life tech). After mild fragmentation, samples were divided into input and IP sample, and IP samples were subjected to m6A immunoprecipitation. RNA from both the input and IP samples was reverse transcribed with a sequence specific RT primer to include the 10 nt unique barcode sequence of each construct in the MPRE. PCR amplicons of these fragments were generated.