GSM7507058: TIAL1_iCLIP_B cells; Mus musculus; RIP-Seq
RIPA buffer and sonication (10s, x3) was used for cell lysate and clarification. Protein:RNA complexes were immunoprecipitated for RNA extraction and small RNA library preparation L3-ATT-App DNA Linker (/5rApp/WN ATT AGA TCG GAA GAG CGG TTC AG/3Bio/) linker primer and irCLIP_ddRT primer_19 (/5Phos/WWW GTATT NNNN AGA TCG GAA GAG CGT CGT GAT/iSp18/GGA TCC/iSp18/TAC TGA ACC GC) or irCLIP_ddRT primer_13 (/5Phos/WWW CCGGA NNNN AGA TCG GAA GAG CGT CGT GAT/iSp18/GGA TCC/iSp18/TAC TGA ACC GC) were used for library preparation as previously described for iCLIP