GSM7910698: AOC11-LN; Mus musculus; RNA-Seq
RNA was isolated from the cells using TRIzol reagent (Invitrogen). mRNA sequencing libraries were prepared using the TruSeq Stranded mRNA Sample Preparation Kit (Illumina, San Diego, CA, USA), according to the manufacturer's protocols. Briefly, mRNA was purified and fragmented from 2 μg total RNA. The libraries were sequenced as paired-end reads (2 × 100 bp) using NovaSeq 6000 (Illumina).