GSM7026797: B6_12M_LG2; Mus musculus; RNA-Seq
Lacrimal glands were lysed into a tube containing RNA lysis buffer (Qiagen, Valencia, CA,USA). Total RNA was extracted using a QIAGEN RNeasy Plus Micro RNA isolation kit according to the manufacturer's instructions. The concentration and purity of RNA were assessed using a NanoDrop 1000 (ThermoFisher Scientific, Waltham, MA, USA). RNA-Seq was performed by the Beijing Genomics Institute (BGI) using the BGISEQ500RS to generate 100 bp paired-end reads.