GSM8147883: BMDM_MP_rep1; Mus musculus; ATAC-seq
Cells were lifted in with Accutase (ThermoFisher) and gentle cell scraping. 5x10^4 cells were utilized to prepare nuclei. Cell membranes were lysed using cold lysis buffer (10mM Tris-HCL pH 7.5, 3mM MgCl2, 10mM NaCl, and 0.1% IGEPAL CA-630). Nuclei were pelleted by centrifugation for 10 minutes at 500 × g, and suspended in the transposase reaction mixture (25 µl of 2X TD Buffer (Illumina), 2.5 µl of TD Enzyme 1 (Illumina), and 22.5 µl of nuclease-free water). The transposase reaction was performed for 30 minutes at 37 °C in a thermomixer shaker at 800RPM.  Then, fragmented DNA in the reaction was purified using MinElute PCR purification kit (QIAGEN). Purified DNA fragments were amplified by PCR to obtain ATAC-seq libraries with Illumina Nextera sequencing primers.