GSM7730664: OV90_P6B_2D; Homo sapiens; RNA-Seq
Total DNA was isolated using Qiagen DNeasy Blood and Tissue Kit (ref 69504). Total RNA was isolated using Trizol/Chloroform extraction method and RNA quality was validated on RNA-1000 chip using Bioanalyzer (Agilent). 1.0 ug of total RNA was used for the construction of sequencing libraries. For DNA, Novogene utilized Agilent SureSelect Human All Exon V6 Kit. For RNA, Novogene utilized the NEBNext UltraTM RNA Library Prep Kit for Illumina.