GSM6703212: Replicate1_Patient; Homo sapiens; OTHER
FFPE Visium slides were deparaffinized and decrosslinked according to manufacturer's protocol (10X Genomics). Probe hybridization was performed using Visium Human Transcriptome probe kit. Probe ligation, release and extension were performed according to the manufacturer's protocol (10X Genomics) The spatially barcoded ligated probe products were harvested and were indexed via Sample Index PCR per manufacturer's protocol (10X Genomics). The resulting libraries were cleaned up using SPRIselect and was subjected to 151bp paired-end sequencing on Illumina NovaSeq 6000.