GSM7882202: mESC QKO, DNMT3A1-W330R, Emseq, R1; Mus musculus; OTHER
Genomic DNA was purified using the NEB Monarch Genomic DNA Extraction kit and 10 ng sheared to an average fragment size of ~300 bp for enzymatic conversion. Prior to sonication, fully 5mC methylated pUC19 (0.01 ng) and fully unmethylated lambda DNA (0.02 ng) were spiked into each reaction to monitor conversion efficiency. After nine cycles of PCR, library quality and quantification were assessed on the Agilent Tapestation 4200. Libraries were sequenced on an Illumina NovaSeq 6000 to generate 100-bp paired-end reads.