GSM7596442: Control A; Saccharomyces cerevisiae; RNA-Seq
The extraction of total RNA was carried out by employing the Direct-zol RNA Miniprep kit (Zymo, catalog number R2052) in conjunction with Ambion TRI-reagent (Fisher, catalog number AM9738) and Zirconia/Silica 0.5 mm homogenization beads (Biospec, catalog number 11079105Z). To remove any contaminating DNA, the samples were treated with Dnase I (NEB, catalog number M0303S). TruSeq RNA Library Prep Kit v2 (Illumina RS-122-2001), Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads.