GSM7142711: PA14_EV_25J; Pseudomonas aeruginosa; Homo sapiens; RNA-Seq
Total RNA was isolated with the miRNeasy Mini Kit (Qiagen, Germantown, MD, Cat. # 217004), including the on-column DNA digestion. RNA was ribosome-depleted prior to library prep. Sequencing libraries were prepared with an Illumina Stranded mRNA Prep kit (Illumina). 150-bp single-end reads were generated using an Illumina MiniSeq.