GSM7754888: Healthy_2_KC; Homo sapiens; RNA-Seq
Keratinocytes were purified by dispase separation of the epidermis (overnight at 4°C, 5U/mL), which was then chopped and digested with 0.25% trypsan-EDTA for 15 minutes at 37°C. Cells were then sort-purified as live CD45- scRNASeq was performed using the 10x Single Cell 3' v3 Chemistry