GSM6469545: ATAC-Nono_KO2; Rattus norvegicus; ATAC-seq
Nuclei were isolated from cells for each replicate, followed by the transposition reaction in the presence of Tn5 transposase (Vazyme) for 45 minutes at 37°C. ATAC-seq libraries were prepared following a published protocol (Liu et al. 2017). Nuclei were isolated, permeabilized, and tagmented using Tn5 transposase (Vazyme), reverse-crosslinked, and amplified using NEBNext High-Fidelity 2 x PCR Master Mix (NEB). Library preparation and sequencing were performed by HiSeq X Ten (paired-end 150bp reads) .