GSM8322816: 19336X16 spore forming 4; Mus musculus; RNA-Seq
RNA extraction: After tissue was collected 350uL of Qiagen buffer RLT+ beta mercaptone ethonal was added and incubated at room temperature for 30 min for lysis before being stored in the -80C. RNA was extracted with the RNeasy micro kit from Qiagen with on column DNase I digest and eluted in 14uL RNAse free H2O. Samples have been stored at -80C. NEBNext Ultra II Directional RNA Library Prep with rRNA Depletion Kit (human,mouse,rat)