GSM6742802: A2783; Mus musculus; RNA-Seq
Mice were perfused with cold PBS, and half of each brain tissue, excluding olfactory and cerebellum, were immediately dissected and dissociated using Neural Tissue Dissociation Kit (P) (Miltenyi). Myelin was removed after tissue dissociation using Myelin Removal Beads II (Miltenyi). Dead Cell Removal Kit (Miltenyi, USA) was applied to dead cells and debris. Libraries were prepared using the Chromium Next GEM Single Cell 3' Kit v3.1 and Chip G Single Cell Kit (10x Genomics, USA) according to manufacturer's protocol.