GSM7027627: MC38, Free R848, rep2, scRNAseq; Mus musculus; RNA-Seq
Mouse MC38 tumor samples were digested utilizing the Tumor Dissociation kit (Miltenyi Biotech) 24h post administration. Cells were then stained with CD45 microbeads and CD45+ and CD45- cells were separated using MACS Separator. CD45+ and CD45- single cells for each mouse were loaded in a ratio of 9:1 onto Seq-Well arrays Single-cell suspensions were loaded onto Seq-Well arrays and processed as according to Gierahn et al., Nature Methods (2017).