GSM8192539: EMseq_501mel shNIPBL_#1 6D rep2; Homo sapiens; OTHER
High-molecular-weight genomic DNA was extracted using Puregene cell core kit (Qiagen). EM-seq was performed with 100ng of purified genomic DNA using NEBNext® Enzymatic Methyl-seq Kit (NEB, E7120) and following manufacturer's instructions. After quantification and size distribution assessment using a Qubit Flex fluorometer (Thermo Fisher Scientific) and a TapeStation (Agilent), respectively, the purified libraries were sequenced on an Illumina NovaSeq 6000 to generate around 200 M PE100 reads per library.