GSM6467587: HI47P404882; Homo sapiens; RNA-Seq
The “PAXgene Blood miRNA Kit” (Qiagen) was used for the purification of miRNA  and total RNA. 100ng total RNA was depleted for rRNA and globin by using QIAseq® FastSelectTM RNA Removal Kit (Qiagen). The RNA Sequencing library was prepared with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). The libraries were sequenced on Illumina NovaSeq6000 using NovaSeq6000 S2 Reagent Kit (100 cycles, paired ended run) with an average of 50 Mio reads per RNA sample.