GSM7507641: SKN2_Non_infected_Rep2; Mus musculus; RNA-Seq
Treated MEFs were washed with PBS, and RNA was harvested using Quick-RNA prep kit (ZYMO research). RNAseq libraries were constructed with Illumina Truseq RNA Sample Preparation kit v2 according to the manufacturer's instructions and sequenced on a HiSeq 2500 at 1x50 base pairs to a depth of 20-40 million reads. Each RNAseq condition was performed in triplicate, using treated MEF cells with 3 different experiments.