GSM7750377: 4T1, ShTAZ 1; Mus musculus; RNA-Seq
Nucleospin RNA, MACHEREY-NAGEL A bulk adaptation of the MARS-Seq protocol was used to generate RNA-seq libraries. Briefly, 30ng of input RNA from each sample was barcoded during reverse transcription and pooled. Following Agencourt AMPure XP beads cleanup (Beckman Coulter), the pooled samples underwent second strand synthesis and were linearly amplified by T7 in vitro transcription. The resulting RNA was fragmented and converted into a sequencing-ready library by tagging the samples with Illumina sequences during ligation, RT and PCR. Libraries were quantified by Qubit (Thermo Fisher Scientific) and TapeStation (Agilent). MARS-seq