GSM7026587: U2AF1WT rep3; Homo sapiens; RNA-Seq
Total RNA was extracted using Trizol LS (Thermo Fisher, Waltham, MA) and RNeasy mini kit (Qiagen, Hilden, Germany). Genomic DNA was removed using Turbo DNA-Free Kit (Thermo Fisher). Stranded libraries were generated using an input of 500ng total RNA and the TruSeq Stranded Total RNA kit with Ribo-Zero Gold, set A, RS-122–2301 (Illumina, San Diego, CA) according to the manufacturer's instructions.