GSM7146860: Patient A4 healthy synovial spatial transcriptomics; Homo sapiens; OTHER
Unfixed human synovial biopsies were snap frozen in OCT Tissue TEK (Sacura). 10 µm thin sections were cut on a Cryotome FSE (Thermo Fisher) Spatial transcriptomes was generated according to the Visium Spatial Gene Expression Kit (v1 chemistry, 10x Genomics). An optimisation slide was run for optimal permeabilisation conditions. 6 min was found to be optimal. Spatial transcriptomes were prepared according to the Visisum protocol CG000239 Rev B). Libraries were sequenced as PE150 by Illumina sequencing to >80% saturation by the external provider Novogene.