GSM8286606: Sham; Mus musculus; RNA-Seq
Adult C57BL/6J females were subjected to Sham or MI surgery (n=5 per group). 7 days after surgery, brains were collected and processed into single cell solutions using a Neuronal Tissue Dissociation Kit (P) following manufacturer's instructions. Myelin debris was removed by density gradient centrifugation and biological replicates were pooled together by condition for processing in the 10X Genomics Chromium System. A targeted number of 10,000 cells were loaded for each experimental condition. Single cell mRNA sequencing libraries were prepared using the 10X Genomics Chromium Next GEM Single Cell 3′ Library and Gel Bead Kit (v3.1) pipeline following manufacturer protocols. Library was performed following manufacturer's instructions (Single cell 3' v3, 10X Genomics)