GSM7429238: resistant2; Homo sapiens; RNA-Seq
For RNA extraction, total RNA from each biopsies obtained as described above were isolated using the standard TRIzol protocol (Invitrogen, Carlsbad, CA, USA). RNA quality was examined by gel electrophoresis and with a Nanodrop spectrophotometer (Thermo, Waltham, MA, USA). Strand-specific libraries were constructed using the TruSeq RNA sample preparation kit (Illumina, San Diego, CA, USA), and sequencing was carried out based on the Illumina novaseq-6000 instrument. The raw data was handled by Perl and data quality was checked by FastQC v0.11.2 TruSeq RNA sample preparation kit (Illumina, San Diego, CA, USA)