GSM8245256: HN_ATAC_039_human_naiveTh_donor3; Homo sapiens; ATAC-seq
Ten thousand cells were pelleted and washed with 50 μL 1 × PBS. After lysis, nuclei were pelleted by centrifuging at 500 × g for 10 min, and re-suspended in 50 μL transposase mixture (25 μl of 2x TD buffer, 2.5 μl of TDE1, 0.5 μl of 1% digitonin, 22 μl of nuclease-free water) (#20034197, Illumina; #G9441, Promega) for incubattion at 37°C with shaking at 300 rpm for 30 min. The tagmentated DNAs were then purified using a QIAGEN MinElute kit and amplified with 10 or 11 cycles of PCR based on the amplification curve The libraries were purified using a QIAGEN PCR cleanup kit, and sequenced for 50 cycles (paired-end reads) on a HiSeq 3000 or NovaSeq 6000 (Illumina)