GSM7596274: OCT6_KO_EpiLCs_Rep2; Mus musculus; RNA-Seq
Cells were washed once in PBS and whole cell lysates were prepared using Trizol reagent (Invitrogen). Total RNA was obtained according to manufacturer specifications. Total RNA was used to prepare libraries with TruSeq Stranded Total RNA LT Sample Prep Kit (Gold) (Illumina) according to the TruSeq Stranded Total RNA Sample Prep Guide, Part # 15031048 Rev. E protocol, followed by paired-end sequencing