GSM7430693: C2C12-Nkd cells, no Dplate, biol rep 1; Mus musculus; RNA-Seq
For RT-PCR analysis, total RNA from cell lines was extracted using the RNeasy Mini Kit (Qiagen) following cell-lysate homogenization via QIAshredder column. mRNA was isolated using polyA selection and converted into sequencing libraries using the NEBNext Ultra II RNA Library Prep Kit following the manufacturer's protocols.