GSM8242529: Epidermis, female_-/-_T24_52F; Mus musculus; RNA-Seq
RNA was harvested using RNeasy Mini Kit (Qiagen) RNA quality was assessed on a Fragment Analyzer (Agilent Technologies). The RNAs had  RQNs between 6.4 and 8.4. RNA-seq libraries were prepared from 500 ng of total RNA with the Illumina TruSeq Stranded mRNA reagents (Illumina). Library preparation was performed on a Sciclone liquid handling robot (PerkinElmer; Waltham, Massachusetts, USA) with a PerkinElmer-developed automated script. Unique dual indexes were used for barcoding of the libraries. Libraries were quantified by a fluorimetric method (QubIT, Life Technologies) and their quality assessed on a Fragment Analyzer (Agilent Technologies).