GSM8323291: Day0_replicate3; Mus musculus; RNA-Seq
At designated times (day 0, day 1, day 3, day 11) cells were removed from Matrigel. Briefly, media was removed and washed with ice cold PBS. TrypLE Express (Gibco; 12604013) was added to wells and incubated at 37°C, followed by scraping of Matrigel bubbles to collect in a 15 mL conical tube. Cells were mechanically disrupted by pipetting ~10 times and incubated at 37°