GSM8244934: dTom_1-GEX; Mus musculus; RNA-Seq
4 Con and 4 mA1 largest KPC tumors were dissected at 16 weeks pti, minced using a razor blade, and incubated at 37°C for 30 min in dissociation solution containing DNAse (1µg/ml, Sigma-Aldrich) and Collagenase IV (200 U/ml, Sigma-Aldrich), prior to filtering through Flowmi Cell Strainer (BAH136800040, Sigma-Aldrich), and dead cells removal (MACS dead cell removal kit, 130-090-101, Miltenyibiotec). Cell Hashtagging was performed using CD45 and MHC Class I TotalSeq-B antibodies (anti-mouse Hashtag 1_ACCCACCAGTAAGAC, 155831, and Hashtag 2_ GGTCGAGAGCATTCA, 155833, BioLegend) and multiplexed samples were submitted for 3P 10x Chromium sequencing Library was prepared using 10x Single Cell 3 Prime V3 kit following the manufacturer's instructions