GSM8150425: ATAC-seq_G3SEKO_BM1_IL18Ra+ST2-PD1+IL17RB-; Mus musculus; ATAC-seq
Lungs were cut into small pieces and digested in RPMI containing collagenase A and Dnase I using gentleMACS Dissociator and then passed through 70 µm filter. Lung ILC2s were sorted by SONY SH800. Bone marrow cells were harvested and lineage-positive cells were depleted using MojoSort Mouse Hematopoietic Progenitor Cell Isolation Kit. Each fraction of ILC2 lineages were sorted by BD Melody. ATAC-seq library was generated using Omni-ATAC protocol.