GSM7142539: D2, BCRseq; Homo sapiens; RNA-Seq
Single cell suspension was performed according to Chromium Next GEM Single cell V(D)J Reagent kits v2. The positive fractions of memory B cells were collected in 6ul of FCS containing eppendorf tubes that were pre-coated overnight with 2 percent BSA. The 5'scRNA-seq libraries were generated using Chromium Next GEM Single Cell V(D)J Reagent Kit v2 (10X Genomics) according to the manufacturer's protocol. Paired heavy and light chain BCR libraries were prepared from the sorted B cell populations. All the steps were followed as per the manufacturer's instructions in user guide recommended for 10X Genomics kit v2.