GSM8192350: + induction_INPUT; Mus musculus; ChIP-Seq
For fixation, the cells were treated with 1% PFA at 37 °C for 15 minutes. To stop the fixation process, 0.125 M glycine was added and incubated for 5 minutes at room temperature. Following fixation, the cells were washed with cold PBS supplemented with 1 mM PMSF and lysed with ChIP SDS lysis buffer (50 mM Tris-HCl, pH 8.1, 10 mM EDTA, 1% SDS) containing protease inhibitor and phosphatase inhibitors for 15 minutes on ice. To achieve DNA fragmentation within the desired range of 200-800 base pairs, an ultrasonic processor (DIAGENODE) was used on ice. Hieff NGS®MaxUp II DNA Library Prep Kit for Illumina® (YEASEN,Shanghai,China）was used for library preparations.