GSM8287104: Early Log yeast, replicate 1, NETseq; Saccharomyces cerevisiae; OTHER
Yeast cells were cyogenically harveste and lysed. RNA Polymerase I immunoprecipitaion was perfomed followed by total RNA extraction using acidic phenol/chloroform. Following RNA extraction, a DNA linker with a UMI barcode was ligated onto the 3' end of the RNA reads. RNA was fragmented and reverse trasnscribed. cDNA was circularized and amplified using Illumina N(i5) and (i7) indices. Libraries were then cleaned with PCRClean DX beads and sequenced.