GSM7506940: sample_41019081703639; Homo sapiens; Bisulfite-Seq
DNA was extracted starting from 500µl following the protocol supplied by the manufacturer. DNA libraries were prepared using the NEBNext UltraII library prep kit (New England Biolabs) and custom, pre-methylated Dual-Unique-Index adapters from Integrated DNA Technologies (IDT) were used for adapter ligation. Groups of 16 libraries, each carrying a different adapter, were pooled together, concentrated by drying with a SpeedVac system and used for targeted enrichment with custom made 5'-biotinylated oligos (IDT).