GSM7428919: mouse ovary, FMR1 knockout, input, biol rep3 [InpKO_3]; Mus musculus; RIP-Seq
5 pairs of ovaries homogenized in MCB buffer (150 mM KAc, 1% Triton X-100, 1% NP40, 50 mM HEPES at pH 7.4, 2 mM MgAc2, 10% glycerol, EDTA-free protease inhibitor, RNase inhibitor [Thermo Fisher Scientific, 10777019]), spun at max speed for 10 min. From each sample, 10% of the homogenate saved as in-put for western blotting, 10% saved as in-put for RNA extraction and sequencing. Illumina library prep