GSM8149558: liver, 10CBD, long-term,2; Mus musculus; ssRNA-seq
Tissues were collected and immediately submerged in stayRNA™ (A&A Biotechnology) reagent. After 24 of cooling (4C), tissues were frozen at -80C until the time of RNA isolation. For RNA isolation, tissue scrapes (15-30 mg) were homogenized and used for RNA isolation with AllPrep DNA/RNA (Qiagen) kit. Following isolation, RNA was controlled for quality with the TapeStation4150 system and quantified. Libraries were prepared using CORALL mRNA-Seq V2 (Lexogen) following standard protocol. RNA-Seq, stranded