GSM8149463: Patch cell Sample 685, Pomc+, Chow diet; Mus musculus; RNA-Seq
At the end of the recording, each cell in the pipette was aspirated for 3 min. The pipette was then broken into an RNAse-free 200 µL Eppendorf tube containing the reaction mix from the SMART-Seq® Single Cell Kit (Takara, 634472). cDNA synthesis was performed according to SMART-Seq® Single Cell Kit (Takara, 634472) and the manufacturer instructions, and libraries have been prepared with the Nextera XT DNA Library Prep Kit (Illumina, San Diego, USA). scRNA-seq PATCH-seq