GSM8286277: CS12, YS, CITE-seq; Homo sapiens; OTHER
The generated cells from cultured CS12a-YS, CS12a-EP, CS12a-YS (CD45+CD127+) and CS12a-YS (CD45+CD127–) were sorted for CITE-seq. Libraries were prepared with Chromium Single cell 3' Reagent v2 Kits accoding to the manufacturer's instructions. Antibody-bound oligos acted as synthetic transcripts that were captured by using 10X Chromium Next GEM Single Cell 3′ Kit v3.1 as per standard protocol and sequenced on the Illumina Novaseq 6000 system.