GSM6797130: HL60, METplus, H3K36me3, Abcam; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes, followed by quenching and cell lysis. Next, cells were sonicated using a Bioruptor (Diagenode) and samples were spiked with mouse chromatin. ChIP-seq libraries were generated using the KAPA Hyper Prep Kit (Roche Sequencing and Life Sciences) according to manufacturer's protocol and sequenced on an Illumina NextSeq500 using default parameters