GSM7731252: HEL cells, LRCherry2.1-sgDCAF15#2, acetyl-SMC3, ChIP; Homo sapiens; ChIP-Seq
Cells were fixed with 1% paraformaldehyde in PBS for 10minutes; after quenching and cell lysis, chromatin was sonicated using a Covaris Ultrasonicator ChIP-seq libraries were prepared using the NEBNext Ultra II DNA library prep with sample purification beads (NEB, E7103S) following the manufacturer's instructions