GSM7911265: brain,RNaseR,nanopore,rep2; Homo sapiens; OTHER
NEB Monarch Total RNA Miniprep Kit was used for home made HEK293 cell RNA, guanidine thiocyanate tecnniques were reported to be used for commercially obtained RNA and Quick-cfRNA Serum & Plasma Kit from Zymo Research was used for RNA extraction from plasma Total RNA or rRNA depleted, RNaseR treated RNA were reverse transcribed with a group II intron RT then the cDNAs were sized selected with SPRI beads. Second strand synthesis and amplification were done with Phi29 DNA polymerase. T7 endonuclease I was used to digest the branched DNA into double stranded DNA and ONT and PacBio library preparation kits for dsDNA were used to make libraries for sequencing.