GSM6704064: Tuft cells, Balb, untreated [UG12]; Mus musculus; RNA-Seq
Cells were sorted directly into buffer from SMART-Seq4 and cDNA was prepared according to manufacturers protocol Libraries were prepared using NexteraXT DNA Library kit according to manufacturer's protocol Barcoded libraries were pooled and quantified using a Qubit® Fluorometer (Life Technologies). Single-read sequencing of the pooled libraries was carried out on a HiSeq2500 sequencer (Illumina) for 74 cycles, using TruSeq v3, and SBS kits (Illumina). Target read depths were ~5-10 million raw reads per sample.