GSM8108153: HeLa, gammaH2AX, siDDX3X, CUT&Tag, rep2; Homo sapiens; OTHER
DNA was purified with minElute PCR kit (Qiagen, 28004). DNA was size-selected firstly by 0.55× DNA clean beads to remove the fragment above 600 bp and secondly by 2× DNA clean beads to get the DNA fragment between 100 bp to 600 bp. To generate the sequencing libraries, DNA was mixed with barcoded i5 primer and barcoded i7 primer and amplified with high-fidelity master mix (NEB, M0541S). Finally, libraries were cleaned by 1.3× DNA clean beads.