GSM7595827: Drosophila_Neuroblast lineage_Control_rep3; Drosophila melanogaster; RNA-Seq
Cells were lysed using ribonuclease inhibitor and lysis component, and cDNA was synthesised by Oligo-dT primer. In summary, cDNA was amplified by the Smart Seq2 method and 40 ng cDNA was used for the construction of sequencing libraries. The qualified cDNA was sheared randomly by ultrasonic waves for Illumina library preparation protocol including DNA fragmentation, end repair, 3' ends A-tailing, adapter ligation, PCR amplification, and library validation.