GSM8322586: N2, 4C 5d, rep 2; Caenorhabditis elegans; RNA-Seq
Animals were washed off the plates with M9 buffer, followed by three washes with M9 buffer before being frozen in liquid nitrogen. Total RNA was extracted by Trizol treatment. Note that total RNA was extracted from the same lysate used for the corresponding ribosome profiling experiment. Library preparation was performed using the ScriptSeq v2 RNA-seq library preparation kit according to the manufacturer's protocol.