GSM8147800: Control; Mus musculus; RNA-Seq
Visium Spatial RNA-seq: Frozen tissue sections from mouse hearts were mounted on 10X Visium slide capture areas, each with 5000 barcoded RNA capture spots. Hematoxylin and eosin (H&E)-stained images of whole tissue sections were obtained by using the BioTek Cytation 5 with image stitching. Optimal permeabilization time for the release of mRNA from 10-μm thick tissue sections for both genotypes was 12 minutes, determined by using the Visium Tissue Optimization Kit. Spatially barcoded cDNA libraries were generated from mRNA released from each tissue section by using the Visium Spatial Gene Expression Reagent Kit, before being sequenced on an Illumina NovaSeq 6000 with 150 cycle paired-end setting (read 1 = 28 cycles, read 2 = 90 cycles, index 1 = 10 cycles, index 2 = 10 cycles).