GSM7882181: ESCC Organoid, H2AK119Ub, Cut&Tag, R1; Mus musculus; OTHER
See Cut&Tag in methods for detailed protocol. In short, cells were collected, washed, and immobilized to concavalin beads, permeabilized with digitonin and incubated with antibody overnight. Next the cells were incubated with Tn5 protein which was activated to digest and tag around the protein bound DNA. The Tn5 tagged DNA-protein complexes diffuse out of the cells and the DNA was purified using PCR purification kit. The Tn5 tagged DNA was PCR amplified and purified with Ampure XP beads.