GSM7882061: RH4_P3F_FKBP12_DMSO_6h; Homo sapiens; OTHER
For HiChIP, the Dovetail MNase HiChIP kit (Catalogue #21007) was used. Briefly, cells were fixed with DSG and 37% formaldehyde, treated with MNase, and immunoprecipitated with anti-RNA Pol2 antibody (Santa Cruz Biotechnology, Mouse mAb #sc-47701 X). On-bead bridge incorporation and religation was performed, followed by reversal of crosslinks, proteinase K treatment, and DNA purification. Mouse C2C12 spike-in was added to the samples for normalization. Standard Illumina barcodes were introduced during library preparation.