GSM7910761: Gr4_CDAA_HFD_90mpk_L_286_EC014_77_Liver_S17_L001; Mus musculus; RNA-Seq
RNA was extracted using QIAzol reagent followed by chloroform extraction followed by an additional purification step using RNeasy mini kit with a DNAse digestion (Qiagen). For each sample, total RNA was normalized to 100 ng before undergoing library preparation using the Illumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus kit per manufacturer's instructions. The Agilent Bioanalyzer and Qubit Fluorometer were used to libraries were qualified and quantified using the the Agilent Bioanalyzer and Qubit Fluorometer prior to pooling samples. Samples were sequenced on the Illumina Novaseq 6000 using 2x100 cycles, per manufacturer's instructions.