GSM8245159: scRNAseq from keloid patient No.3; Homo sapiens; RNA-Seq
Keroid tissues were dissociated in a gentleMACS C-tube (Miltenyi Biotec) with digestion buffer composed of DMEM (Gibco) and Multi Tissue Dissociation Kit 1 using a gentle MACS Octo dissociator (Miltenyi Biotec). After running the program “37°C_multi_H”, cell suspensions were passed through a 70-μm nylon mesh sheet. Libraries were prepared using Chromium Single Cell 3' Reagent Kits v3.1 following the manufacturer's protocol (10X Genomics, CA, USA). The generated scRNA-seq libraries were sequenced using a total of 127 cycles (paired-end reads) with a NovaSeq 6000 (Illumina, Inc., CA, USA).