GSM7026662: 96 cells per library, ipsc-motor neurons, ATXN2-ALS and healthy control iPSC-derived hMN combined, 2c; Homo sapiens; RNA-Seq
RNA was extracted by 2x heat lysis (65C) in 384 well and immediately used for downstream single cell RNA sequencing reactions (Celseq2) as previously described (Hashimshony et al., 2016 and Muraro et al., 2016). RNA libraries for RNA-seq were prepared following a SORT-seq pipeline