GSM6703833: U3121, cells, GBM (ATACseq); Homo sapiens; ATAC-seq
For ATAC-seq, an exact number of 50,000 cells for each of the 16 cell lines were used to tagment DNA using Illumina Tagment DNA TDE1 Enzyme and Buffer Kits (small) (Illumia #20034197). Exactly 5uL of tagmented DNA from each line was amplified with NEBNext High-Fidelity 2x PCR Master Mix (New England Biolabs #M0541S). Custom oligos and adapters were designed and used for library amplification. Fragmentation of the libraries were assessed by running Bioanalyzer High Sensitivity DNA Analysis (Agilent # 5067-4626) and were sent to be sequenced at Novogene.