GSM6704230: DC95_GM_CSF_FL_d16; Mus musculus; RNA-Seq
GM-CSF DCs on day 8 of the differentiation protocol and GM-CSF + FLT3L DCs on day 16 were harvested. In addition, GM-CSF DCs or GM-CSF + FLT3L DCs were treated with AZD5582 or the same volume of DMSO for 24 hours. DCs were harvested, washed and cell pellets were snap-frozen. RNA isolation and sequencing was performed by CeGaT. Briefly, total RNA was isolated using RNeasy Mini Kit (Qiagen). Quantity of RNA was measured by Qubit (Thermo Fisher) and quality profile was made using the Fragment analyzer (Agilent). Total RNA samples having RIN>8 were subjected to library generation. The library was prepared using TruSeq Stranded mRNA kit (Illumina) according to manual and analyzed using the Fragment analyzer. The libraries were sequenced on NovaSeq6000 (Illumina Inc.). Total RNA samples having RIN>8 were subjected to library generation. The library was prepared using TruSeq Stranded mRNA kit (Illumina) according to manual and analyzed using the Fragment analyzer.