GSM7142411: A4; Mus musculus; RNA-Seq
Total RNA was isolated using miRNeasy® Mini Kit (Qiagen) according to the manufacturer's protocol. Briefly, 50-60 mg of crushed frozen liver from female mice were homogenized in QIAzol lysis reagent (RNeasy Plus Mini Kit, Qiagen) and then transferred to a RNeasy® Mini column to be further processed with a genomic DNA elimination step by following the manufacturer's RNA isolation protocol. Total isolated RNA was tested to fit high quality and integrity standards, as determined by the 28S/18S rRNA peak and area ratios (ranges 1.8-2.1 and 1.9-2.5, respectively) and the RNA integrity number (RIN, range 8.2-8.7) using RNA 6000 Pico Assay RNA chips run in Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA). 200 ng total RNA was used for mRNAseq library preparation using the NEBNext Poly(A) Magnetic Isolation Module and NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs, Ipswich, MA, USA).