GSM7730880: MiE-8; Mus musculus; RNA-Seq
Mice were transcardially perfused with PBS and the cortical tissues were quickly dissected and enzymatically digested using the Neural Tissue Dissociation kit (P) (Cat#130-092-628, Myltenyi Biotec) at 37˚C in the gentleMACS™ Octo Dissociator with Heaters (Myltenyi Biotec). The myelin was then removed using magnetic bead separation (Myelin Removal Beads II, Cat#130-096-733, Myltenyi Biotec). After myelin removal, samples were subjected to red blood cell removal (Cat.# 130-094-183, Miltenyi Biotec), followed by dead cell removal  (Cat. #130-090-101, Miltenyi Biotec) according to the manufacturer's instructions. The final cell pellet was suspended in Ca/Mg-free PBS supplemented with 0.5% FBS for downstream analysis. In all samples, more than 90% of the cells were viable based on trypan blue examination. Libraries were prepared using the Chromium platform (10x Genomics) with the 3′ gene expression (3′ GEX) V3 kit with a targeted input of ~ 6,000 cells per sample following the manufacturer's instructions.