GSM7749680: mT_gastrodermis; Aurelia coerulea; RNA-Seq
Whole embryos, animals, or dissected tissues were  incubated in calcium-magnesium free artificial sea water, followed by an incubation in 300μl Collagenase/Dispase Blend I (Merck) at RT for 30 minutes.  Mechanical force was used to disrupt the tissues and obtain single-cell suspensions. Cell suspensions were washed with 0.1% BSA (bovine serum albumin), and cell viability and counts were assayed with a Cellometer X2 (Nexcelom). Cell suspensions were diluted to 1000 cells/ microliter and loaded into a 10x Genomics single cell platform using vs2 or vs3 reagents. 10X Genomics Chromium v2/v3 chemistry according to manufacturer protocols