GSM6703838: U3009, cells, GBM (H3K27Ac.ChIPseq); Homo sapiens; ChIP-Seq
Approximately 3-6 million cells were cross-linked with 1% formaldehyde for 15 minutes After quenching and cell lysis, chromatin was sonicated using a E220 focused-ultrasonicator (Covaris) for 12 minutes, IP was done with 4ug of anti-H3K27Ac: ab4729 About 10ng of immunoprecipitated DNA from H3K27Ac/ H3K27me3-ChIP was used to prepare libraries using NEBNext Ultra II DNA Library Prep Kit for Illumina, E7645L and NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs), E6440S.