GSM6128180: H3K4Me3_BC3_Hypoxia; Homo sapiens; ChIP-Seq
Cells were harvested by centrifugation and washed by resuspending them in PBS. Washed cells were lysed in hypotonic lysis buffer followed by passing through syringe and centrifugation to isolate nuclei. Isolated nuclei were resuspended in detergent containing buffer to lyse the membrane followed by sonication for fragmentation of chromatin. size of fragmented chromatin was estimated by runnig on agarose gel. Libraries were prepared according to Illumina's instructions using ChIP sequencing sample preparation kit following the manufacturer's protocols.