GSM7731188: KI673_mRNA_heart_54818; Mus musculus; RNA-Seq
Hearts were perfused with 100 mM KCl and removed for RNA extraction. Total RNAs were extracted with Trizol LS (Invitrogen, 10296010), treated with DNaseI, and purified. For tamoxifen time course experiments, mRNA sequencing libraries were generated using the poly-A selection module in the NEBNext UltraII Directional RNA Library Prep Kit (NEB E7760) and sequenced on the Illumina HiSeq 2500 sequencer with single-end 50 cycles. For cGas/Lmna double knockout experiments, libraries were generated using QuantSeq 3' mRNA-Seq Library Prep Kit FWD (Lexogen, K01596) and sequenced on the Illumina NovaSeq 6000 sequencer with single-end 100 cycles.