GSM8245535: zebrafish embryo,28C,A0,1hpf,total; Danio rerio; RNA-Seq
Total RNA was isolated using tri-reagent (Sigma T9424) and quantified using nanodrop We used a two-step RNA-Seq library construction approach that specifically targeted the variable 3′UTR region of reporters. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the 20nt downstream 3′