GSM7785558: mESC Control 24h Replicate 1; Mus musculus; RNA-Seq
Total RNA  from three replicates of each condition was isolated with TRIzol (Invitrogen) and DNA was degraded with TURBO DNase (Invitrogen), according to the manufacturer's protocol. RNA spike-ins (Invitrogen) were added to each purified RNA sample. PolyA RNA-seq libraries were produced from 1 ug of RNA using the TruSeq® Stranded mRNA Library Prep kit (Illumina), according to the manufacturer's instructions. The libraries were paired-end sequenced using the NovaSeq 6000 system at 100-bp length, according to the manufacturer's protocol.