GSM8108113: MCD-2; Mus musculus; RNA-Seq
C57BL/6 mice and CD73 KO mice were fed with NCD (normal control diet) and MCD(methionine/choline-deficient diet) for 4 weeks. Immune cells in liver samples were stained and detected by fluorescence activated cell sorting (FACS) cells Aria II (BD Biosciences, CA, USA), CD4+ CD25+ cells (Treg) were selected. Trizol reagent was used for collecting total RNA extraction. RNA libraries were prepared for sequencing using standard Illumina protocols.