GSM6742808: Lung_ILC2_SS2_DA2; Mus musculus; RNA-Seq
RNA were extracted from cells using RNAClean XP beads (Backman) For bulk RNA-seq: 2,000 ILC2s from each indicated condition were lysed in 10 μl TCL buffer plus 1% 2-mercaptoethanol. Libraries were processed with SMARTSeq2 with three biological replicates per condition, and paired-end sequenced (150bp × 2). For scRNA-seq: Flow sorted murine lung ILC2s which were pre-labeled with hashtag antibodies(Biolegend), were pooled and loaded at 60,000 cells per chanel. The cells were then partitioned into the GemCode instrument, where individual cells were lysed and mixed with beads carrying unique barcodes in individual oil droplets. The products were subjected to reverse transcription, emulsion breaking, cDNA amplification, and sample index attachment for paired-end sequencing (150bp × 2).