GSM7028129: WT strain, rep2; Saccharomyces cerevisiae; OTHER
Cells were fixed with 1% formaldehyde for 20 minutes. After quenching, cell wall was digested using zymolyase. Cells  were fixed with 3 mM disuccinimidyl glutarate for 45 min and quenched. The chromatin was digested using Mnase, and the DNA was labeled using biotin-dATP and biotin-dCTP. After ligation, biotinated DNA was purified using agarose gel electrophoresis followed by streptavidin beads purification. ChIP-seq libraries were prepared using the KAPA Hyper Prep Kit complemented with xGen™ Stubby Adapter and UDI Primers (IDT).