GSM7679916: GF_HFD_retina_DS15; Mus musculus; RNA-Seq
Whole mouse retinas were isolated on ice from freshly enucleated eyes, with all equipment, surfaces, and tubes treated with RNase decontamination solution (Thermo Fisher Scientific, Waltham, MA, USA) prior to use. Dissected retinas were stored in RNAlater solution (Thermo Fisher Scientific, Waltham, MA, USA) at −80 °C until RNA extraction using the RNeasy kit from Qiagen (Qiagen, Hilden, Germany). Concentrations were quantified using a Nanodrop (Thermo Fisher Scientific, Waltham, MA, USA) before sequencing. The quality was evaluated using a Bioanalyzer at the University of Chicago Genomics Core and was confirmed to meet appropriate RNA integrity numbers (RIN). Next, cDNA libraries were constructed using TruSeq RNA Sample Prep kits (Illumina, San Diego, CA, USA) to generate 100-bp paired-end reads, which were indexed for multiplexing and then sequenced using PE100bp on the NovaSeq 6000 System (Illumina, San Diego, CA, USA).