GSM8111911: scBSseq_293T_P8_C2; Homo sapiens; Bisulfite-Seq
Individual cells were directly sorted into 96-well plates containing Buffer RLT Plus (Qiagen, 1053393) and incubated for 5 minutes at 4°C. EZ-96 DNA Methylation-Direct MagPrep Kit (ZYMO, D5045) was then used to process DNA bisulfite conversion. Bisulfite-converted DNA was amplified with random primer and klenow exo– polymerase (Qiagen, P7010-HC-L) for 5 rounds and excess primer was removed by AMPure XP beads (Beckman, A63881). After second strand synthesis, the library was amplified by KAPA HiFi HotStart ReadyMix (Kapa Biosystems, KK2602) for 14 cycles.