GSM7060937: INPUT_WT_Ncor2_PD3_rep1; Mus musculus; ChIP-Seq
ChIPseq: cells were fixed in 1% FA for 10min and fixation was quenched by 0.125 M glycine at RT. Cells were scraped in PBS, centrifuged at 500g for 5min and pellet was resuspended in an ice-cold IP-buffer, a 2:1 mixture of SDS-buffer (100mM NaCl, 50mM Tris-Cl pH 8.0, 5mM EDTA pH 8.0, 0.5% SDS) and Triton-X-buffer (100mM NaCl, 100mM Tris-Cl pH 8.6, 5mM EDTA pH 8.0, 5% Triton X), containing complete Protease inhibitor tabs (11873580001, Sigma). After 30min incubation on ice chromatin was sonicated 15s on (pulsed, with 90% actual sonication time) /30s off for 20 cycles using the UW2070 sonicator (Bandelin) at ~40% power. 30µg and 10µg chromatin (DNA equivalent) were precipitated using the listed antibodies.