GSM8193368: pigu_homo_bud_PGCs_RNA_rep2; Danio rerio; RNA-Seq
Total RNA of oocytes were purified using RNeasy Mini kit (QIAGEN, 74104) as recommended protocols. Total RNA (5 μg) was DNase I (Fermentas, EN0521) treated at 37°C for 1 h. Poly-A tailed mRNA was collected using DynabeadsTM mRNA Purification Kit (Invitrogen 61006). RNA-seq was carried out with the SMART-seq2 protocol, as previously described, and subjected to paired-end sequencing on the Nextseq 500 (Illumina) platform according to the manufacturer's instructions. Notably, SMART-seq2 of low-input RNA-seq relies on poly(A)-based amplification.