GSM7060405: TFAP2A_KO_rep3; Homo sapiens; OTHER
Cells were removed from the plate and singularized with Accutase. Cohesin HiChIP library prep was performed as previously described (Mumbach et al., 2016). Briefly, 25x106 cells were crosslinked and digested with MboI (NEB). After digest, biotin was incorporated into the sticky ends of fragments before ligation. Cohesin ChIP was performed to enrich for proximity ligations bound to cohesin, using an SMC1 antibody (Bethyl, A300-055A). The paired-end library quality was assessed on a MiSeq sequencer before sequencing on an Illumina HiSeq 4000. Three replicates were pooled and sequenced across two HiSeq lanes for a total of 1200 million reads per sample.