GSM7060182: EndoC-βH1, Control, biol_rep 3; Homo sapiens; RNA-Seq
Total RNA was extracted using the Qiagen miRNeasy isolation kit. RNA concentration and quality was determined using 2μL on a NanoDrop Spectro-photometer RNA-seq libraries were prepared using the TruSeq Stranded Total RNA Library Prep with Ribo-Zero kit (#RS-122-2201, Illumina, CA, USA). Samples were loaded and sequenced using the Nextseq 500/550 High Output kit v2 (#FC-404-2002, Illumina, CA, USA) on an Illumina NextSeq 500 sequencer