GSM8287267: Mouse blastocysts cultured from E1.5 to 4.5 in M16 medium with 0.01% Fast Green FCF, Replicate 1; Mus musculus; RNA-Seq
Total RNA was isolated using ReliaPrep RNA Cell MiniPrep System (Promega). Ion Ampliseq Transcriptome Mouse Gene Expression Kit (Life technologies), Ion Ampliseq Transcriptome Mouse Gene Expression Core Panel (Life technologies) and Ion Barcode Adaptors 33-48 Kit (Life technologies) were used for the construction of seqeuncing libraries. Libraries were ampified on beads by emulsion PCR using Ion Chef system (Life technologies). Sequencing was performed with Ion540 Kit Che (Life technologies) and Ion 540 Chip.