GSM7731949: c587ts;UAS-upd,testis; Drosophila melanogaster; ChIP-Seq
The required adult testes were dissected and cross-linked with 1% formaldehyde for 15 minutes. After quenching and cell lysis, chromatin was sonicated using the Covaris system. Most chromatin fragments occurred between 200-400bp. The process of dilution, antibody incubation, protein G pull down, beads washing, DNA complex elution, de-link,RNAse A/proteinase K digestion and DNA extraction are all performed according to standard protocols.