GSM8148215: Step Down 10 mM to 2 mM, rep 3 [SD3]; Staphylococcus epidermidis ATCC 12228; RNA-Seq
We harvested RNA using enzymatic lysis (lysostaphin and mutanolysin), mechanical disruption (TissueLyser LT Qiagen 85600), and an RNeasy Mini Kit (Qiagen 74106).  ≥ 500ng of total RNA was used for the construction of sequencing libraries. We used Novogene Co., LTD (Beijing, China) to carry out our rRNA depletion, cDNA library construction, and sequencing as part of their Prokaryotic RNA Sequencing service.