GSM7881924: LOTUS21_1yr; Homo sapiens; RNA-Seq
Frozen liver cell suspensions were thawed, filtered through 40um mesh and sorted for alive (DCM acqua, Thermofisher) CD45+ cells (CD45-BV711, Biolegend). Maximum of 12500 cells/sample were loaded for scRNAseq. Libraries of extracted cells were prepared with Chromium Next GEM Single Cell 3' kit (10x Genomics). Libraries were converted to circular ssDNA using the MGIEasy Universal Library Conversion Kit (MGI Tech) for sequencing on the DNBSEQ platform.