GSM7027488: 900_PASC_WT; Homo sapiens; RNA-Seq
Following incubation, total RNAs were extracted from PBMCs using the AllPrep kit as per manufacturer's instructions (Qiagen). Briefly, following oligo dT enrichment, fragmentation and random priming, cDNA syntheses was completed. End repair, 5' phosphorylation and dA-tailing were performed, followed by adaptor ligation, PCR enrichment, and sequencing.