GSM6742906: A2780-294D; Mus musculus; OTHER
One hemisphere of mouse brain used for Visium was embedded in optimal cutting temperature (OCT) in a cryomold and lowered into isopentane cooled with liquid nitrogen until the OCT was completely frozen. The OCT embedded tissue block were stored in a sealed container at –80°C until cryosectioning. Mouse brains were cryosectioned (Center for Biologic Imaging, University of Pittsburgh) at 10 μm and placed directly onto the Visium slides ensuring placement within the capture area. To ensure sampling of different brain regions, two sections were used from each mouse with the first section 500µm caudal to the first appearance of the dente gryus, and each 10-μm section was 500 μm apart. Visium tissue optimization and spatial gene expression protocols were followed exactly as described by 10x Genomics (https://10xgenomics.com/) using immunofluorescence as the counterstain.