GSM7731509: 95; Danio rerio; RNA-Seq
Adult fish were euthanised by sudden immersion in an ice water slurry for at least ~30 seconds before decapitation. The whole head was submerged in RNAlater solution for 24 hours at 4°C and stored at -80°C. The brain was dissected from the RNALater preserved head. Then total RNA was extracted from the brain using Qiagen RNeasy columns. RNA samples were QC'd by PE Labchip for quality and Qubit for quantity. Libraries were generated according to the Nugen Universal Plus mRNA-seq (Part No. 0508) protocol M01442 v2 and included 13 cycles of amplification. Cluster generation was performed using llumina to MGI Library Conversion (MGIEasy Universal Library Conversion Kit, Part No. MGI1000004155. protocol M01442 v2 and included 13 cycles of amplification.