Illumina NovaSeq 6000 paired end sequencing; IL-2Rα-biased, but not βγ-biased, agonist reprograms tumor immune landscape and synergize with αPD-1 therapy
Small tumors (60-80mm3) and large tumors (200-300mm3) with or without the indicated IL-2 treatment (treated for 3 days) were extracted for RNAseq analysis.  Total RNA was isolated from each sample using the RNA mini kit (Qiagen, Germany). RNA quality was examined by gel electrophoresis and with Qubit (Thermo, Waltham, MA, USA).  For RNA-sequencing, 1μg of total RNA was used for library construction. Sequencing libraries were generated using VAHTSTM Total RNA-seq (H/M/R) Library Prep Kit for Illumina.