GSM7506764: Lung, FASNKO, rep 2; Mus musculus; RNA-Seq
RNA was extracted using TRIzol reagent (Invitrogen) and was purified by the RNeasy Plus Mini Kit (Qiagen), together with DNA digestion with the RNA free DNase set (Qiagen).RNA quality was determined by 260:280 ratio and the RNA integrity number (RIN) determined by an Agilent Technologies 2100 Bioanalyzer. Only high quality RNA samples with a 260:280 ratio > 1.6 and a RIN > 7 were used for the library construction using the TruSeq Stranded mRNA Library Preparation kit (Illumina), according to manufacturer's instructions. RNA libraries were prepared for sequencing using standard Illumina protocols