GSM7789446: M6, recovery sleep, male, rep3; Mus musculus; RNA-Seq
Nuclei were isolated from the dissected POA tissues using Minute Single Nucleus Isolation Kit for Neuronal Tissues/Cells (Invent Biotechnologies, Inc., BN-020) following manufacturer's protocol with minor modifications. Specifically, Protector RNase inhibitor (Sigma, PN-3335399001) was added at a concentration of 0.2U/µl to all the buffers provided by the kit. libraries were prepared using the 10x Genomics Chromium Single Cell 3' Reagent kit v3 per manufacturer's instructions. Libraries were uniquely indexed using the Chromium dual Index Kit, pooled, and sequenced on an Illumina NovaSeq 6000 sequencer in a paired-end, dual indexing run.