GSM8149321: c201e3 ovoid female control; Bos taurus; RNA-Seq
TRIzol™ and Phasemaker™ Tubes (ThermoFisher) were used to extract high purity total RNA from whole, individual ovoid (n = 6, IL6-treated; n = 6, non-treated, control) and tubular (n = 4, IL6-treated; n = 4, non-treated, control) conceptuses. Samples were further processed for RNA sequencing at Vanderbilt Technologies for Advanced Genomics (VUMC, Nashville, TN, USA) using the NEBNext® rRNA Depletion kit (New England BioLabs Inc., Ipswich, MA, USA). A sequencing assay was carried out in a NovaSeq 6000 System (Illumina, Inc, San Diego, CA) to produce pair-end reads 150 nucleotides long.