GSM8191335: ULK1_3; Mus musculus; RNA-Seq
Total RNA was isolated from hippocampal tissues from WT, 5xFAD, 5xFAD;Ulk1OV and Ulk1OV mice. Agarose gel electrophoresis was applied to check RNA integrity and DNA contamination; RNA purity (as well as concentration) was checked by the NanoPhotomerer spectrophotometer (IMPLEN) and Bioanalyzer 2100 system (Agilent Technologies). Then cDNA libraries were constructed using ≥ 800 ng RNA per sample with the Illumina's NEBNext® UltraTM RNA Library Prep Kit following manufacturer's recommendations. The effective concentration of the library was determined to be at least 2nM. Next-generation sequencing data (performed by the company Novogene, China) were obtained and analyzed. FastQC and trim galore were used to filter raw sequence data and the removal of adapters, N-containing reads, and low-quality reads (reads below Q20). . The effective concentration of the library was determined to be at least 2nM. Next-generation sequencing data (performed by the company Novogene, China) were obtained and analyzed. FastQC and trim galore were used to filter raw sequence data and the removal of adapters, N-containing reads, and low-quality reads (reads below Q20).