GSM6404264: Ribo_WT_St2; Mus musculus; OTHER
The amygdala was isolated from the brains of control and stressed mice and quickly frozen in liquid nitrogen. The samples were lysed in ice-cold lysis buffer. For RNA-Seq, TruSeq Stranded mRNA Library Prep Kit (Illumina) was used. For ribosome profiling, RNase I treatment, ribosome pelleting, size selection, preadenylated linker ligation, rRNA depeletion, reverse transcription, cDNA circularization, and PCR amplification were conducted.