GSM7595875: HaCaT shRNA 2; Homo sapiens; RNA-Seq
Poly A RNA was isolated from 1 µg total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England BioLabs, Ipswich, MA), and enriched with SMARTer Apollo automated NGS library prep system (Takara Bio USA, Mountain View, CA). The cDNA library was made using the NEBNext Ultra II Directional RNA Library Prep kit (New England BioLabs) PCR cycle number of 8, followed by library QC and quantification via real-time qPCR (NEBNext Library Quant Kit, New England BioLabs). The individually indexed libraries were proportionally pooled