GSM7731431: Primary Astrocyte, Replicate 1; Homo sapiens; RNA-Seq
Total RNA was extracted from the embryonic stem cells, derived hypothalamic neural progenitors and primary astrocytes with the Zymo Direct-zol RNA miniprep kit. Total RNA was extracted from hypothalamus tissue with the Zymo Quick-DNA/RNA Miniprep Plus kit. Libraries were prepared using the QIAseq FastSelect RNA Removal kit, NEBNext Ultra II Directional RNA Library Prep for Illumina kit, and NEBNext Oligos for Illumina (Dual Index Primers Set 1), according to manufacturer's instructions.