GSM6704375: (B) Steady state neutrophils, after infection, rep 3; Danio rerio; RNA-Seq
Larvae were homogenised in 0.25% trypsin with mechanical agitation prior to FACS-isolation of 1000 neutrophils per sample. cDNA was synthesised directly from cell lysates using the SMART-Seq v4 Ultra Low Input RNA kit for sequencing (Takara Bio) Libraries were made using the Nextera XT DNA Library Preparation Kit (Illumina).