GSM7596619: INPUT H3K4me3 trb45 plant; Arabidopsis thaliana; ChIP-Seq
1g of 7-day old in vitro grown plantlets were fixed in 1% formaldehyde under vacuum twice for 7 min and then quenched in 0.125 M glycine. Nuclei were isolated and lysed, and chromatin sonicated using the Diagenode Bioruptor (set to high intensity, 3 times 7 cyles (30sec ON / 30 sec OFF) or the S220 Focused-ultrasonicator (Covaris) 5 min at peak power 175 W, duty factor 20%, 200 cycles per burst for histone modifications anti-H3K27me3, Diagenode, #C15410069, Batch A1818P, anti-H3K4me3, Millipore #04-745 DNBSEQ stranded mRNA library