GSM7595919: MHCC-97H cells, Control, 16h, rep1; Homo sapiens; RNA-Seq
Total RNA was harvested using Rneasy mini plus kit (Qiagen) Enrichment of mRNA with Oligo (dT) Beads, Adding Fragmentation Buffer to Randomly Break mRNA, Using mRNA as a template, synthesize the first cDNA strand and second strand, and purify the cDNA, The purified double stranded cDNA is then subjected to end repair, A-tailed addition, and sequencing connectors are connected. Then, AMPure XP beads are used for fragment size selection, and finally, a cDNA library is obtained through PCR enrichment