GSM7028804: HL60_shDDX6_Input_1; Homo sapiens; OTHER
For genome wide screen, genomic DNA was extracted using phenol/chloroform For RNA- and polysome-seq, total and polysomes RNAs were extracted using TRIzol DNA library for Genome wide screen was performed as previously described by Michlits et al, 2020 Nature Methods (DOI:  10.1038/s41592-020-0850-8) RNA libraries for RIP-seq were constructed using NEBNext Ultra II Directional RNA library prep and Unique Dual Index Primer Pairs RNA libraries for total and polysomes RNA-seq were constructed at Beijing Genomics Institute (BGI Group)