GSM7881663: Tumor after FLASH proton irradiation; Mus musculus; RNA-Seq
Mice bearing medulloblastoma tumors were euthanized and perfused with PBS supplemented with EDTA. Tumor tissues were harvested and digested with collagenase-II and DNase. Single cell suspension was prepared after filtering using a mesh strainer with 100-μm pores. Cell samples were prepared and analyzed following a manufacturer's V3 library protocol (10x Genomics) and single-cell RNA-seq at the next-generation sequencing core at the Children's Hopital of Philadelphia.