GSM8322551: MSCs from MCB, passage 3, donor 199, sequenced by RNAseq; Homo sapiens; RNA-Seq
Cells were used and treated with the PureLink RNA Mini Kit (Thermo Fisher). The RNA concentration was measured with nanodrop (Thermo Fisher). Each sample was split into two tubes (25 ul each) and concentration varied between 50 and 2,760 ng/ml. Conventional RNA-Seq libraries were prepared by Genwiz from Azenta Life Sciences an MACE-seq libraries were prepared by GenXPro GmbH MACE-Kit V2 RNA-seq Massive Analysis of cDNA Ends (MACE-Seq)-RNA sequencing