GSM6797066: RWPE, PSIP1-sh RNAseq Rep3; Homo sapiens; RNA-Seq
RNA was isolated using an RNeasy mini kit (Qiagen Cat no: 74106). Then mRNA was isolated using Poly (A) mRNA magnetic isolation kit (NEB cat no: E7490L) RNA seq library was prepared using NEBNext Ultra II directional RNA library preparation kit (NEB cat no: E7760L) by following the manufacturer's protocol. Libraries were sequenced as 150 bp paired-end reads using Novaseq 6000.