GSM7731291: IL12(I)GFP in vivo R1; Homo sapiens; RNA-Seq
Total RNA was extracted from NKTs using the Rneasy Plus Mini kit (Qiagen) per manufacturers instructions. Messenger RNA libraries were prepared (TruSeq Stranded mRNA LibraryPrep, Illumina) and sequenced on the Illumina HiSeq4000 platform (UNC High-Throughput Sequencing Facility) using paired-end 100-bp reads, with 44 million reads on average (range, 1.5-149 million).