GSM6742607: WT (XEFW1.2f), VPA/CHIR99021, repA; Mus musculus; RNA-Seq
Intestinal organoids were retrieved from Matrigel using ice-cold PBS, washed 2 to 3 times to remove dead cells, and collected using a benchtop centrifuge. Total RNA was extracted with the RNeasy Plus Micro Kit (Qiagen Cat# 74034). 1.4 ug of total RNA was used for the construction of sequencing libraries. First-strand cDNA was generated following a poly(A) enrichment protocol, and the resulting cDNA libraries were sequenced as 100 bp paired-end reads on the HiSeq 2500 System (Illumina). Library preparation and sequencing were performed by the Oxford Genomics Centre.