GSM7728642: ATAC-Seq MV411, DMSO, 6h, ATACseq_Rep1; Homo sapiens; ATAC-seq
Cells were pelleted, washed with DPBS, and nuclei were extracted with a hypotonic buffer. Extracted nuclei were transposed for 30 minutes with Tn5 transposome in buffer containing digitonin, NP-40, and Tween-20. ATAC-seq libraries were amplified by high-fidelity PCR with barcoded Nextera indices.