GSM7429587: control RPMI rep 2; Homo sapiens; RNA-Seq
Cells were collected and stored in RNALater (#76106, Quigen). Total RNA was extracted using Rneasy Mini RNA isolation kit (#74134, Qiagen) following Qiagen protocol, with the following modifications: (1) lysis time is increased up to 40 min; (2) on-column DNase I treatment is performed. cDNA libraries were constructedusingthe NEBNext Ultra II RNA Library Prep Kit for Illumina (cat#E7770S/E7770L) by NEB followingthe manufacturer's protocol.