GSM8322802: HeLa cells, siUSP50-2; Homo sapiens; OTHER
Cells were harvested in Dulbecco's phosphate-buffered saline (PBS) and counted. Cells (120,000 per well) were adhered onto a poly-L-lysine–coated 96-well plate and cross-linked in methanol-free paraformaldehyde (final concentration 4%) for 10 min. The PFA was removed, and the wells were washed twice in PBS and stored in 200 μl PBS. Plates were sealed and stored at 4°C until downstream library preparation Library and INDUCE-Seq protocol are here: https://doi.org/10.1038/s41467-022-31702-9 INDUCE-Seq DNA double-strand break sequencing.