Illumina HiSeq 2000 paired end sequencing; RNA-seq from heart tissue of 192 heterogeneous stock rats
Hearts were collected for RNA extraction. Poly(A)-purified mRNA-seq libraries were generated from high quality RNA (average RNA Integrity Number (RIN) of 9.1). RIN scores were measured on a BioAnalyzer 2100 using the RNA 6000 Nano assay (Agilent; 5067-1511). RNA-seq library preparation was performed in batches of maximum 48 samples according to the TruSeq Stranded mRNA Reference Guide (Illumina Cat. No. RS-122-2101,RS-122-2102), using 1000ng of total RNA as input.