GSM7429345: Dura_OG_IV_scRNA; Mus musculus; RNA-Seq
Extravascular CD45+ cells were sorted from digested dura or colon tissue for RNA extraction and library preparation. Gene expression libraries were prepared from FACS-sorted populations of single cells using the 10x Chromium Controller and 10x Chromium Single Cell V(D)J 5' kit v2 with TCR amplification (10x Genomics, Inc.). Samples were loaded onto the chip following manufacturer's recommendations.