GSM7680320: MN22_bone_cell_S45; Canis lupus; RNA-Seq
RNA was extracted from tumor tissue at baseline, and from tumor tissue samples obtained at amputation, post-treatment. RNA was also extracted from cell lines that were established pre-treatment from each case, and from normal skin tissue from each case as a non-malignant control. When available, RNA was also extracted from lung metastases. Tissue samples were disrupted with a tissue homogenizer, and RNA was extracted using a commercially available kit (QIAGEN RNeasy mini kit). RNA was stored at -80°C. Dual-indexed sequencing libraries were prepared using the Clontech Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian kit. (2x50-bp paired-end, on a NovaSeq S2