GSM7910467: SLX-22619.B5_Ven_res_3_hs; Homo sapiens; RNA-Seq
697WT and 697KI were harvested, washed and RNA was extracted from 4×106 cells using the RNeasy kit (Qiagen). Libraries were prepared using NEBNext Poly(A) mRNA magnetic Isolation module (NEB, E7490) starting with 1µg DNA-free RNA, as per manufacturer's instructions. RNA fragmentation, double-strand cDNA synthesis, end repair, adapter ligation and PCR amplification (9 cycles) was performed using NEBNext Ultra II directional RNA library prep kit for Illumina protocol (NEB, E7760) according to manufacturer's protocol. Library quality and molarity was measured by Qubit (ThermoFisher) and TapeStation (Agilent). Samples were 50-bp paired-end sequenced on the NovaSeq (Illumina) instrument (CRUK CI Genomics Core Facility).