GSM7429267: muscle, 24 months old, wildtype, rep_5; Mus musculus; RNA-Seq
M. gastrocnemius muscles were crushed and tissue was further homogenized using Lysing matrix D tubes (MP Biomedicals) in TRI Reagent (Sigma). RNA was purified using chloroform phase separation and ethanol cleanup. Libraries were prepared from total RNA using an Illumina TruSeq RNA Library Prep Kit (version 2).