GSM7731424: KI983_SLAMIT_heart_0392; Mus musculus; OTHER
Mice were sacrificed, and hearts were perfused with 100 mM KCl and removed. RNAs from the left ventricular free wall were extracted by Trizol LS and purified and DNaseI treated using Directzol RNA miniprep kit (Zymo, R2052). Purified RNAs (1.5 µg) were subjected to alkylation in the presence of 12 mM Iodoacetamide (IAA, Sigma I1149). High-throughput sequencing libraries were generated from the alkylated RNAs using Quant-seq 3' mRNA-seq FWD kit (Lexogen K01596) and sequenced 100 bases on the single-end mode on an Illumina NovaSeq6000 sequencer.