GSM6797176: posterior epiblast, E8.5, WT, rep1; Mus musculus; ATAC-seq
Tissue samples were incubated with accutase for 30 minutes to dissociate into single cell suspension. After lysis and transposition reaction, genomic DNA was extracted with MinElute purification kit (Qiagen) ATAC-seq libraries were prepared using NEBNext High-Fidelity 2× PCR Master Mix with custom-synthesized PCR primers (Buenrostro et al, 2013). Libraries were amplified using 8 cycles in the thermocycler. The amplified libraries were size selected using SPRIselect beads from Beckman Coulter.