GSM8191462: HFD fed mice without STZ treatment, 32w, batch 9, biological replication 3; Mus musculus; RNA-Seq
Total RNA was isolated using a RNeasy Mini kit (Qiagen, Hilden, Germany; 74104) in keeping with the manufacturer's instructions. The RNA sequencing library was prepared using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs, Ipswitch, MA, USA; E7760).