GSM7506762: 17_LV_mCherryNegative; Mus musculus; OTHER
Splenocytes from humanized mice were sorted into mCherry+ and mCherry- primary human T cells using an SH800S cell sorter (Sony Biotechnology). RNA was extracted from human primary T cells using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. TCR a/b libraries were prepared from each sample using the SMART-Seq Human TCR (with UMIs) kit (Takara Bio) according to the manufacturer's instructions.