GSM6468130: Ana_S2; Mus musculus; RNA-Seq
The VE-cadherin+ cells were labeled with tdTomato by injecting tamoxifen (200 μg/g) at postnatal day (PD)17, and dorsal skin was collected at PD20, PD25 and PD32. The dorsal skin was digested in collagenase and Dispase mixture as previously described (Chovatiya et al., 2021). The dead cells were removed by LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit (ThermoFisher). FACS Aria (BD Biosciences) was used for the cell sorting. Single-cell 3′ cDNA libraries were generated using Chromium Single Cell 3′ gel bead and library Kit v3. Libraries were generated following the manufacturer's protocols (10x Genomics).