GSM7061099: PBS_NRF2_ChIP_1; Homo sapiens; ChIP-Seq
Peripheral blood mononuclear cells were isolated from sickle cell disease pateint and CD34+ cells were enriched by microbeads and cultured in a Fibach two-phase cultured system. On day 10 of culture, cells were either treated with PBS or cell permeable trifluoromethylbenzyl ester derivative of L2HG or PBS for 48 hours. On day-12 of culture, cells were crosslinked with 1% formaldehyde, sonicated, and immunoprecipitated with NRF2 antibody and subjected to library preparation using TruSeq ChIP Sample Preparation kit (Illumina) for the construction of sequencing libraries.