GSM7849880: Poly_Female_WT_2; Mus musculus; RNA-Seq
RNA was harvested using Rneasy mini plus kit (Qiagen). 100 ng and 200ng for polyTIL  and OT1 samples respectively of total RNA was used for the construction of sequencing libraries. For RNA-seq PolyTIL RNA libraries for RNA-seq were prepared using Takara SMART-Seq v4 Ultra low Input RNA kit following manufacturer's protocols. And for RNA-seq OT1 RNA libraries for RNA-seq were prepared using illumina TruSeq mRNA-Seq Library Prep Kit following manufacturer's protocols.