GSM7910932: DY, scRNAseq; Homo sapiens; RNA-Seq
PBMCs were extracted by Ficoll gradient centrifugation. Whole blood cells were diluted at a 1:1 ratio with 1× PBS before being layered onto lymphocyte separation medium and subjected to centrifugation at 500g for 20 minutes at 4°C. The PBMC-containing white-cloud layer was carefully isolated using capillaries and subsequently washed twice with PBS after removal of erythrocytes. For each sample, the cell viability exceeded 80%.10x Genomics® Cell Preparation Guide describes best practices and general protocols for washing, counting and concentrating cells from both abundant and limited cell suspensions (greater than or less than 100000 total cells, respectively) in preparation for use in 10x Genomics Single Cell Protocols. RNA libraries were prepared for sequencing using standard Illumina protocols