GSM7429922: mccc-1_Coma_biorep2; Caenorhabditis elegans; RNA-Seq
A minumum of 200 staged animals were collected and washed thrice in M9 buffer. Total RNA was extracted via Trizol and BCP followed by Dnase I treatment. Zymo Direct-zol RNA miniprep kit was used for clean up. BGI standard protocol