GSM7816142: D90, CMZ, snRNA; Homo sapiens; RNA-Seq
Wash the samples twice with PBS, add 0.5 ml of digestion solution (containing 0.25% Trypsin-EDTA, 50 μg/ml DNase I, 20 μg/ml Y-27632), and digest at 37°C for 10 minutes. Add 1 ml of culture medium to neutralize the digestion, filter through a mesh filter, centrifuge at 1000 rpm for 5 minutes, remove the supernatant, and resuspend in an appropriate volume of suspension solution (containing PBS, 50 μg/ml DNase I, 20 μg/ml Y-27632). Count and calculate the proportion of viable cells (≥90%), adjust the cell concentration to 1000 cells/μL, and keep on ice for further testing Using the single-cell sequencing platform (10x Chromium Single Cell Platform), perform on-board processing of the cell samples (combining cells/enzymes with Gel beads to form GEMs, cell lysis, cDNA amplification, library construction, etc.)