GSM7730977: 7PPP; Bos taurus; RNA-Seq
RNA was isolated from tissue using using TRIzol reagent (Invitrogen, Carlsbad, California) in combination with a Direct-zol RNA kit (Zymo Research) The RNA-seq was performed using Swift Rapid RNA Library Kit (Biosciences) with deplete rRNA (Ribo-off rRNA Depletion Kit (Vazyme)), according to the protocol. The libraries were ligated with different indexes,  pooled in one pool and sequenced in the 150 pair-end cycles on Illumina NovaSeq.