GSM7911206: icals3m, 72h, estradiol, rep 1; Arabidopsis thaliana; RNA-Seq
Two leaves from four plants (either DMSO or estradiol treated) were harvested 12 h, 24 h, 48 h and 72 h post treatment. Three biological replicates for each genotype/treatment/time point were collected. Leaves were flash frozen in liquid nitrogen. Leaf tissue was homogenised using a Genogrinder and each Eppendorf tube contained 2 steel ball bearings. RNA was extracted from leaf tissue using the Qiagen Plant RNeasy mini kit. Rigorous treatment with Turbo DNase was undertaken to remove genomic DNA contamination. Libraries were constructed by Novogene Co., Ltd using Illumina NovaSeq 6000