GSM7430672: Probiotics Rep1; Rattus norvegicus; OTHER
10 μM fresh frozen brain sections (coronal) of PLC and ILC regions were placed on capture areas of Visium slides. As per manufacturer's protocol (10x Genomics Visium Spatial Gene Expression kit), sections were then methanol-fixed and permeabilized. The released mRNA molecules were reverse transcribed to produce spatially barcoded cDNA which were then amplified. Libraries were prepared and sequenced on the Illumina NovaSeq, PE150. Reads were trimmed to 28 cycles for read 1 and 90 cycles for read 2. 10x Genomics Visium Spatial Gene Expression kit