GSM6467600: keratinocytes from the dorsal skin of Gsda1a3eKO mice treated with water, Day5 [Mut_H2O_1]; Mus musculus; RNA-Seq
The mouse dorsal epidermis was separated from the dermis by treatment with 2.5 U/mL displase in HBSS for 3 hours at 37 oC, then keratinoocytes were isolated by Trysin-EDTA (0.25%, Invitrogen) treatment at 37 oC for 10 min.  Total RNA from keratinocytes was isolated using NucleoSpin RNA mini kit (MACHEREY-NAGEL, CO.) according to the manufacturer's protocol. Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.