GSM7908630: H3K9me3 IP rep1, NIH/3T3; Mus musculus; ChIP-Seq
Genomic DNA of NIH/3T3 cells, B104-1-1 cells were all prepared using Monarch Genomic DNA Purification Kit (NEB, T3010S). All the DNA samples were harshly treated with RNase A (NEB, T3018L) again prior to library construction to get rid of any RNA contaminants. The ChIP-seq grade antibody H3K4me3 (1 µg/ µl) was provided by the iDeal ChIP-seq Kit for Histones, while H3K9me3 (D4W1U, Rabbit mAb, #13969, 1:50 dilution) and H3K27me3 (C36B11, Rabbit mAb, #9733, 1:50 dilution) antibodies were purchased separately at Cell Signaling. The immunoprecipitated DNA was further proceeded with NEBNext® Ultra™ II DNA Library Prep Kit (NEB, E7645S) for library preparation, followed by sequencing at NextSeq 2000 as paired-end 75 bp mode.