GSM7911531: Steatotic Hep G2 cells treated with negative control, sample 3; Homo sapiens; RNA-Seq
RNA was extracted using the miRNeasy Mini Kit (Qiagen, 217004) according to the manufacturer's protocol, followed by RNA concentration determination (NanoDrop 2000, Thermo Scientific), standard gel electrophoresis for quality assesement, and RIN determination (Bioanalyzer 6000, Agilent). RIN was determined to be above 9.5 for all samples. Libraries were constructed from 500ng total RNA using KAPA Stranded mRNA-Seq Kit (KAPA Biosystems Wilmington, USA) and the RNA fraction was sequenced on the NextSeq 500 System (Illumina) at the Center for Genomic Technologies Facility, the Hebrew University of Jerusalem polyA-selection