GSM7429726: F5R8Sept2017; Mus musculus; RNA-Seq
Total RNA was extracted using TRIzol reagent (ThermoFisher) from 5 millions of CD43- spleen B cells For each sample, 500 ng total RNA was reverse-transcribed to cDNA by a 5'RACE PCR using ProtoScript II (New England Biolabs) and a cap race primer associated with consensus reverse primers for each mouse IgH Cm, Cg and Ca CH1 region or the human Cµ CH1