GSM7028004: H3K9ac, Col0, IP, R1; Arabidopsis thaliana; ChIP-Seq
For H3K9ac ChIP-seq, immunoprecipitation was performed similarly as H3K27me3, except that 1.5g mature seeds were used. DNA was extracted using phenol-chloroform method. 20ng ChIP-ed DNA and input DNA were sent to Novogene (China) for sequencing, 150bp paired-end with a minimum25M clean reads per sample.