GSM7911443: Ligand2; Mus musculus; ATAC-seq
Cells were removed from the plate with Trypsin. Nuclear DNA was extracted following ATAC-seq the protocol in Buenrostro et al. 2015. Briefly, 7x104 cells were washed with cold PBS and lysed in 0.1% NP40 RSB buffer. Nuclei were Tn5 transposed with Nextera Transposase at 37°C for 30 minutes, then purified with the Qiagen MinElute PCR Purification Kit. Libraries were amplified for 9-15 total cycles using the Nextera Ad1 and Ad 2.1-2.16 barcodes. Libraries were purified and eluted using the Min-Elute columns (Qiagen). Library concentrations were determined with Bioanalyzer High-Sensitivity DNA analysis (Agilent).