GSM7026649: Spinal cord, Q22;TDP-43 animal (4230); Mus musculus; RNA-Seq
Total RNA was isolated using QIAzol Lysis reagent (Qiagen) and miRNeasy mini kit (Qiagen) with DNA digestion on-column, according to the manufacturer's instructions. RNA concentration and integrity was validated using the RNA 6000 Nano Kit on a 2100 Bioanalyser (Agilent), with RIN values for all extractions above 7.5. Samples were rRNA depleted and prepared for sequencing using SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara). Library size and purity control was performed on a 2100 Bioanalyzer (Agilent) using a High-Sensitivity DNA-Chip. Library concentration was measured using a KAPA Library Quantification Kit.  Prepared libraries were sequenced on three lanes PE100 with 20% in lane control on HiSeq4000