GSM6403951: serum_shortRNA_PH150_w0h0; Homo sapiens; ncRNA-Seq
Skeletal muscle RNA was extracted using Qiagen miRNeasy kit (Cat. No. 217004); serum EV RNA was extracted using Qiagen exoRNeasy kits (Cat. No. 77144). Long RNA libraries for both skeletal muscle and serum EVs were constructed using Takara Bio SMARTer Stranded Total RNA-Seq kit v2 Pico Input Mammalian (Cat. No. 634418) and SMARTer RNA Unique Dual Index Kit (Cat. No. 634452). Small RNA libraries for both skeletal muscle and serum EVs were prepared using NEXTflex Small RNA Library Prep Kit v3 (Ca. No. # NOVA-5132-23, Perkin Elmer