GSM7061049: Bud, WT, sorted cells (S phase), replicate 3; Danio rerio; OTHER
Genomic DNA was prepared from whole embryo pellets or sorted nuclei using the Quick-DNA Miniprep Plus Kit (D4069; Zymo Research). mRNA was prepared from whole embryos using the RNeasy Mini Kit (74104; Qiagen). For whole-genome sequencing, 50ng - 2μg of gDNA was sheared to approximately 300 bp fragments using a sonicator (Covaris). The gDNA libraries were prepared using the KAPA Hyper Prep Kit (Roche) with SeqCap (Roche) or Illumina TruSeq (Illumina) adapters. Genomic DNA libraries were sequenced in 150bp paired-end read fragments on an Illumina HiSeq 2500 or NovaSeq 6000.