GSM6601584: WT Fur 0.5% NaCl, replicate 1; Helicobacter pylori; RNA-Seq
RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit  was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols Libraries were sequenced on a NovaSeq 6000 with paired reads of 150-bp length, according to the manufacturer's protocol.  Bcl2fastq2 conversion software (Illumina) was used to generate de-multiplexed Fastq files. A total of 15 independent samples were analyzed; 8 RNA preparations from Fur-R88H expressing bacteria grown in media containing either 0.5% NaCl (4 independent samples) or 1.0% NaCl (4 independent samples), and 7 RNA preps from cultures of WT Fur expressing strains grown in media containing 0.5% NaCl (4 independent samples) or 1.0% NaCl (3 independent samples)