GSM7679633: bidimensional culture 2; Homo sapiens; miRNA-Seq
RNA was extracted using Trizol reagent (Invitrogen) following the manufacturer's instructions. The quantity and quality of total RNA were evaluated using Nanodrop (ThermoFisher Scientific, USA) and RNA integrity was assessed using agarose gel electrophoresis at 60 V, stained with GelRed 0.5 X, and visualized on a UV transilluminator MicroRNA expression analysis of SKBR3 cells cultured in 2D and 3D culture was performed by whole Small RNAseq. The TruSeq Small RNA Library Prep kit (Illumina, USA) was used for the preparation of the library.