GSM8286147: MEFs, sheIF2B5_sheIF4E_Total_rep4; Mus musculus; RNA-Seq
Mouse Embryonic fibroblast (MEF) was used in the Lentivirus shRNA gene knockdown experiments. Lentiviral shRNAs targeted to eIF2B-epsilon, eIF4E were generated by HEK293 cell, then infected MEF for two days. The infected MEFs were selected by puromycin before RNA purification. RNA was purified by Trizol-LS according to manufactorary instruction before RNA-seq library generation. TruSeq Stranded Total RNA protocol RNA-seq of polysomal and total RNA samples