GSM6601789: WGBS_E8.5 Whole Embryo_Zmym2KO_F_sample2_rep1; Mus musculus; Bisulfite-Seq
Genomic DNA was isolated using the QIAamp DNA mini kit (Qiagen, 51304) or AllPrep DNA/RNA Micro Kit (Qiagen, 82084). 500 ng of genomic DNA and 1.25ng of Lambda spike-in DNA was sheared using the M220 ultrasound sonicator (Covaris) to an average genomic size of 350 bp. 200 ng of sheared DNA was bisulfite-converted using the EZ DNA Methylation-Gold Kit (Zmyo Research, D5005) using manufacturer's instructions. Up to 100 ng of genomic DNA was then used to generate WGBS libraries with the Accel-NGS Methyl-Seq DNA Library prep kit (Swift Biosciences, 30024) according with manufacturer's instructions and 13 cycles of PCR for final library amplification.