Illumina HiSeq 3000 paired end sequencing; RNAseq analysis of Campylobacter jejuni treated with hypochlorite
1.5 ml of culture was collected and centrifuged (12,000 g, 2 min, 4oC). The resulting supernatant was removed, and cell pellets were immediately frozen by submerging in liquid nitrogen for 45 seconds Campylobacter jejuni was grown to steady state in XX medium in an infors bioreactor with feed rate set at XX. Samples were taken pre-treatment with hypochlorite for the T0 sample Hypochlorite (XX mM) was added directly to chemostat RNA isolation was performed by GENEWIZ rRNA depletion and transcript library preparations were performed by GENEWIZ