GSM7060774: 06HUVEC_INPUT_siRNF20_rep2; Homo sapiens; ChIP-Seq
Nuclei were isolated and the chromatin was sheared to yield fragment sizes ranging from 100-400bp. Sheared chromatin was precleared (sepharose G fast flow 4) beads and followed by antibody incubation over night at 4°C. Lysates were incubated with sepharose G fast flow 4 beads at 4°C for  3 h bound DNA washed  2x with low salt, 1x high salt, 1x LiCl and 2x Te buffer. AFter proteinase K digestion and reverse crosslinking DNA was cleaned up with the Zymo kit Libraries were prepared with the NEBNext Ultra II DANN Library Prep Kit for Illumina with the  NEBNext® Multiplex Oligos for Illumina® (96 Index Primers)