GSM7596278: IP GFP WT rep1; Arabidopsis thaliana; ChIP-Seq
1g of 7-day old in vitro grown plantlets were fixed in 1% formaldehyde under vacuum twice for 7 min and then quenched in 0.125 M glycine. Nuclei were isolated and lysed, and chromatin sonicated using the Diagenode Bioruptor (set to high intensity, 3 times 7 cyles (30sec ON / 30 sec OFF) or the S220 Focused-ultrasonicator (Covaris) for 20 min at peak power 110 W, duty factor 5%, 200 cycles per burst for TRB4-GFP anti-GFP, Invitrogen, #A-111222 DNBSEQ stranded mRNA library