GSM7680375: CD8 T cells, HC_primed, d14; Mus musculus; RNA-Seq
Single-cell suspensions from spleens and livers were stained with Viobility 405/520 fixable dye (Miltenyi), with PB-conjugated anti-CD8α (clone 53-6.7) and PE-conjugated anti-CD45.1 (clone A20) antibodies. Live CD8+ CD45.1+ cells were sorted on a MoFlo Legacy (Beckman Coulter) cell sorter in a buffer containing PBS with 2% FBS Single cells were processed on the Chromium platform (10x) using the Chromium Next GEM Single Cell 3' v3.1 (Dual Index). After quality controls and quantification on TapeStation instrument (Agilent), libraries were sequenced on NovaSeq6000 platform (Illumina) generating around 25'000 reads/cell.