GSM6742751: A2547; Mus musculus; RNA-Seq
mRNA were isolated from the mouse brain cortex using Rneasy mini kit (Qiagen), and RNA quality was assessed using 2100 Bioanalyzer (Agilent). After RNA isolation, concentration was measured with a Qubit 3.0 Fluorometer (Thermofisher) before quality assessment with the 2100 Bioanalyzer instrument (Agilent Technologies). Sequencing libraries were generated using mRNA Library Prep Reagent set (Illumina) as previously described.