GSM7596551: Control; Mus musculus; RNA-Seq
Single nuclei were isolated using Pure Prep Nuclei Isolation kit (Sigma, St. Louis, MO, USA) as per manufacturer's instructions. The freshly prepared nuclei were used to prepare sequencing libraries using 10X Genomics Chromium Single Cell 3ʹ GEM, Library & Gel Bead Kit v3.1 at the University of Missouri Genomics Technology Core. The libraries were quantified using a Qubit HS DNA kit. The fragment size was analyzed using an Agilent Fragment Analyzer system.  Libraries were sequenced on an Illumina NovaSeq 6000 with a sequencing configuration of 28 base pair (bp) on read1 and 98 bp on read2. Each library was sequenced to a depth of 20,000 paired-end (single-indexing) reads per nucleus. Single-nuclei RNA-seq