GSM7731277: U-937, vehicle, rep1, RNA-seq; Homo sapiens; RNA-Seq
Cells were collected and pelleted after treatment, and homogenized with a QIAshredder (Qiagen 79654). Total RNA was extracted using RNeasy Plus Mini kits (Qiagen 74136). Qiagen kits were used according to manufacturer's protcols. Total RNA was sent to Novogene for library construction and sequencing. mRNA was enriched for by using poly-T magnetic beads, and library created using NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB E7770).