GSM7595667: sample 1 (B23-0396-001); Mus musculus; OTHER
Mice were anesthetized by intraperitoneal injection of ketamine (80 mg/kg), xylazine (16 mg/kg), and acepromazine (3 mg/kg) and transcardially perfused with cold PBS. Organs were placed in 10% formalin and after 24 h transferred in 70% ethanol before embedding in paraffin blocks. Microtome sections (2-2.5 µm) were cut for hematoxylin and eosin staining. Microtome sections on regular glass slides were deparaffinized and stained for H&E as described in Visium CytAssist Spatial Gene Expression for FFPE (10X Genomics, CG000520). Regions of interest on the tumor sections were then chosen by means of the H&E stain and were aligned in the tissue slide cassette 6.5mm. After destaining and decrosslinking, sections were immediately subjected to probe hybridization overnight followed by probe ligation, release and extension and spatial library construction as described in Visium CytAssist Spatial Gene Expression for FFPE (10X Genomics, CG000495).