GSM7882134: QKO, DNMT3A UBmutant, DNMT3A ChIP R1; Mus musculus; ChIP-Seq
2 × 107 cells were cross-linked directly on a plate after media removal and one wash with PBS, using 1% paraformaldehyde for 10 min at room temperature. Lysed chromatin extracts were sonicated for 20 min using a Covaris M220 focused ultrasonicator. After antibody incubation overnight at 4C the antibody bound chromatin was washed and purified using PCR purificaiton spin column (Qiagen) ChIP–seq, library preparation was carried out using NEBNext Ultra II Library Prep Kit reagents