GSM6704075: DMA-3; Mus musculus; RNA-Seq
Pregnant Nlrp3+/+ and Nlrp3-/- dams were intra-amniotically injected with S100B or PBS on 16.5 days post coitum. Sixteen hours after the injection, the decidual and uterine tissues were collected, followed by the isolation of neutrophils and macrophages from these tissues using fluorescence activated cell sorting. Total RNA was extracted from the sorted cells using the Pico Pure RNA isolation kit (Applied Biosystems) according to the manufacturer's instructions. RNA concentrations, purity, and integrity were evaluated using the NanoDrop 1000 spectrophotometer and the Bioanalyzer 2100. Two to four biological replicates from each group were combined for the neutrophil samples to obtain quantities of RNA for RNA-seq. cDNA was synthetized using Smart-seq2. The Nextera DNA Library Preparation Kit were used to prepare the RNA-seq library according to the manufacturer's instructions.