GSM7911034: Alveolar_macrophages_FilteredAir_8032_Rep1; Mus musculus; RNA-Seq
Alveolar macrophages from mouse lungs were isolated by flow cytometry using an established protocol (PMID 29671604), followed by RNA extraction using Qiagen RNAEasy kits. RNA quality was assessed by Bioanalyzer. Library preparation was conducted using the Roche Kapa stranded mRNA library prep kit and sequenced on an Illumina NovaSeq 6000 using paired-end 50bp reads. One mismatch was used in demultiplexing indexes with bcl2fastq ver 2.20.0