GSM8193420: P19_IR_CONV_9Gy_2; Homo sapiens; RNA-Seq
Total RNA were extracted using RNAeasy Mini Kit from Qiagen. RNA quantity and quality control was assessed by capillary electrophoresis (Bioanalyzer, Agilent). All samples have a RIN > 7. Libraries were prepared from 500 ng of total RNA using the Illumina TruSeq Stranded mRNA Library preparation kit according to manufacturer recommendation. 100 bp paired-end sequencing was performed with the Illumina NovaSeq 6000 instrument.