GSM8061836: PDCoV_virion_rep1; Sus scrofa; OTHER
After completing the proximity-ligated, the RNA was extracted by proteinase K, Trizol and chloroform according to the manufacturer's instruction. BioMag®Plus Concanavalin A beads was used to capture virions, 500 μL purified virus was added to the 150 μL washed beads with ConA Binding buffer, and incubated for 10 min at room temperature. The beads was washed with ConA Binding buffer for three times and PBST buffer for once (1 × PBS, 0.1% Tween 20), and resuspended in 1 mL PBST buffer. The final concentration of 1% formaldehyde was used to fix the RNA-RNA interaction, and final concentration 0.125 M glycine was added to quench the formaldehyde.