GSM6601768: D1_2DomLib_WT_CARseq; Homo sapiens; RNA-Seq
Single cell gene expression library preparation was done following the the Chromium Next GEM Single Cell 3' Reagent Kits v3 User Guide (CG000183 Rev C; 10x Genomics). Resulting cDNA was used to do a target amplification of the CAR cDNA barcoded transcripts. Such transcripts were then sequenced using PacBio Sequel II T cells were extracted from the co-culture using Fluorescence Activated Cell Sorting (FACS).