GSM7787415: normal lung epithelium cell line 2; Homo sapiens; ncRNA-Seq
total RNAs were extracted from HBE and A549 cells using RNAprep Pure Plant Kit (Tiangen, Beijing, China) and RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Libraries were prepared by Hieff NGS Ultima Dual-mode mRNA Library Prep Kit for Illumina (Yeasen Biotechnology (Shanghai) Co., Ltd.). The library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Library quality was assessed on the Agilent Bioanalyzer 2100 system