GSM7732145: Primary broiler satellite cells, hypoxia, 24 h, biol rep 5, tech rep2; Gallus gallus; RNA-Seq
Cells were harvested immediately before differentiation induction (0 h) and after differentiation (12 h, 24 h, 36 h, and 48 h) for RNA isolation. Total RNA was isolated using Direct-zol RNA Miniprep kit according to the manufacturer's instructions (Zymo Research). Total RNA (500 ng) was reverse transcribed into cDNA using iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer's instructions. Primers were designed based on the chicken refseq genome (Gallus_gallus-6.0/galGal6) using NCBI Primer Blast (https://www.ncbi.nlm.nih.gov/tools/primer-blast) to represent the consensus transcript for each gene and to yield products that spanned exon boundaries. Targeted RNAseq