GSM7061332: ctrl.3; Mus musculus; RNA-Seq
RNA from lysed mammary gland tissue was extracted using the Direct-zol RNA Miniprep kit (Zymo Research, Irvine, CA) according to the manufacturer's instructions. Libraries for whole transcriptome RNA-Seq were prepared using the Sense mRNA-Seq Library Prep Kit V2 (Lexogen, Vienna, Austria) according to the manufacturer's instructions starting with an input of 300 ng of RNA and 13 cycles of final PCR amplification.