GSM7430901: Barcode dataset of sample 1; Mustela putorius furo; RNA-Seq
Embryonic tissue was collected in ice-cold Leibowitz medium and papain dissociation was carried out (Wortington, #LK003150). GFP-possitive cells were FACS sorted and after processed on the 10x Genomic Platform. Gel Beads-in-emulsion and cDNA generation carrying cell- and transcript-specific barcode was done using the Chromium Single Cell 3' Reagent Kit v3.1 (PN-1000121) following the manufacturer's protocol. Transcriptomic libraries were prepared for sequencing using standard Illumina protocols. Barcode libraries were prepared from PCR cDNA to amplify barcode regions with custumed primers with standard Illumina adapters.