GSM8107988: Flu_pig_lung1, replicate 1, scRNA-seq; Sus scrofa; RNA-Seq
Approximately 1 g of tissue collected from the left cranial, middle, and caudal lung lobes were combined and digested with 2.5 mg/mL of Liberase TL in Dulbecco's Modified Eagle Medium at 37oC for 45 minutes, passed through a 100 micrometer cell strainer, and treated with an ammonium chloride-based red blood cell lysis buffer. The resulting cell suspensions were washed, stained with propidium iodide for exclusion of dead cells, and FACS sorted for live cells using a Sony SH800 Cell Sorter. Gene expression library was performed according to the manufacturer's instructions (single cell 3' protocol, 10x Genomics)