GSM7428249: WT_H151_TL8-3; Homo sapiens; RNA-Seq
Cells were pelleted and lysed using 1mL trizol LS, RNA was extracted using total RNA extraction protocol by the Cornell Transcriptional Regulation and Expression (TREx) facility, using chloroform extraction. RNA integrity was confirmed by Fragment Analyzer RNA QC (Agilent). RNAseq libraries were constructed by the TREx Facility at Cornell University using the NEBNext Ultra II RNA Library Prep Kit (New England Biolabs). RNA-seq libraries were prepared from 100ng total RNA using the NEBNext Directional Ultra II RNA Library Prep Kit for Illumina (New England Biolabs), with initial [polyA+ isolation/rRNA depletion].