GSM6523921: Cartilage,  Ossification, 45 day 3; Sus scrofa domesticus; RNA-Seq
The sample was extracted RNA by the RNA extraction Kit(RNeasy Mini Kit ,Cat#74106, Qiagen) Magnetic beads were used to remove rRNA. Next, the RNA removed from rRNA was subjected to fragmentation, double-stranded cDNA synthesis, degradation of the second strand, end repair, addition of A to the 3 'end, junction and amplification experiments.