GSM8286665: siRNA1; Homo sapiens; RNA-Seq
50ul siRNA mixture+750ul Opti MEM, 50ul NC mixture+750ul Opti MEM (siRNA and NC dry powder dissolved in DEPC water; 100ul/1OD, operated on ice), gently mix and let stand at room temperature for 15 minutes. Drop the mixed solution into the corresponding 10cm cell culture dish, return the cells to the incubator for 4-6 hours, and then add 8mL of complete culture medium containing 20% FBS to incubate for 24 hours. A total amount of 1 μg RNA per sample was used as input material for the RNA sample preparations