GSM7679663: MM001, ZEB2, ChIP-seq; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 min. After quenching and cell lysis, chromatin was sonicated used a Diagenode Bioruptor sonicator. ChIP-seq libraries were prepared using the Illumina Truseq DNA sample preparation guide. 15 ng of DNA was used as starting material. Libraries were amplified using 18 cycles on the Thermocycler. Postamplification libraries were size selected at 300-500 bp in length using Agencourt AmPure XP beads from Beckman Coulter. Libraries were validated using the Agilent DNA high sensitivity kit.