GSM7060991: pooled mRNA-derived cDNA and ADT-derived cDNA; Homo sapiens; OTHER
mouseCD45neg humanCD3pos humanCD4pos live cells were FACS-sorted 7 days post-transfer. Purified cells were stained with DNAoligo-conjugated anti-NRP2 antibodies and subsequently loaded onto a 10x controller (10x Genomics). Libraries were prepared using Chromium Single Cell 3ʹ v3 protocol (10x Genomics). Cellular mRNA and antibody-derived oligos were reverse-transcribed and indexed with a shared cellular barcode. Indexed cDNAs were then pooled and amplified by PCR according to the Chromium Single Cell 3' v3 protocol (10x Genomics) and with specific primers amplifying the antibody-derived tags.