GSM7910936: Control1; Canis lupus familiaris; RNA-Seq
For the extraction of total RNA, 1 ml TRIzol (Magen (R4801-02)) was added to the sample ground in liquid nitrogen, and total RNA was extracted by the TRIzol method.  Then, the sample concentration and 260/280, 260/230 ratio were measured and recorded by Nanodrop (ThermoFisher (ND-2000)), and the integrity of RNA was checked by Agilent 4150 (Agilent Technologies Inc). For the library construction, oligo (DT) beads were used to enrich eukaryotic mRNA, fragmentation buffer was added to randomly interrupt mRNA, mRNA was used as template to synthesize two strands of cDNA, and double-stranded cDNA was purified by AMPure XP beads.  The purified double-stranded cDNAs were end-repaired and A-tailed, and then the fragment size was selected by AMPure XP beads.  Finally, the final cDNA library was obtained by PCR enrichment.