GSM8287121: Late Log yeast, replicate 3, RNAseq; Saccharomyces cerevisiae; RNA-Seq
Yeast cells were pelleted and flash frozen. Total RNA was obtained using hot acidic phenol/chloroform extraction followed by DNAse I treatment to deplete DNA from samples. A final acidic phenol/chloroform extraction was perform to remove DNAse I from samples. rRNA was depleted using NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB #E7490). RNA-seq libraries was prepared using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB #E7760) in conjunction with NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1) (NEB #E7600).