GSM8286685: Enriched myeloid cells from mouse 19120, PBS; Mus musculus; RNA-Seq
RNA were extracted using the RNeasy micro kit (QIAGEN) according to the manufacturer's instruction. For each sample, 200 ng of total RNAs was used for the construction of sequencing libraries. RNA quality was assessed by Bioanalyzer (Agilent Technologies) with RNA integrity number (RIN) of ≥ 6.5 were selected for library construction. Illumina TruSeq Stranded Total RNA Library prep kit (#20020596, Illumina) was used to prepare the libraries per Illumina protocol specifications. Ribozero beads, included in the kit, were used to deplete the rRNA. Concentrations of the libraries were determined using the Qubit Flex (#Q33327, Fisher) and the Qubit 1X dsDNA HS Assay kit (#Q33230, fisher).