GSM7908933: MDS_25G1_2; Homo sapiens; RNA-Seq
Sample homogenization was performed using 700 μL of QIAZOL (QIAGEN) by manual crushing with a pestle. After homogenization, two supernatants were collected in new 1.5 mL RNase-free microcentrifuge tubes. RNA extraction was carried out using the miRNeasy Micro Kit with a Rnase-Free Dnase Set (QIAGEN). The cDNA libraries were constructed using the TruSeq Stranded mRNA Library Prep Kit and protocol from Illumina (Illumina Inc., San Diego, CA).