GSM7910989: BC389, PT, scRNA-seq; Homo sapiens; RNA-Seq
Fresh breast tumors tissues from 4 ER+ breast cancer patients were separated from fat tissues and minced into pieces up to 2 mm in diameter with scalpel blades. Single-cell suspensions were prepared by using the gentle MACS Dissociator (Miltenyl Biotec, Auburn, CA, USA) according to the standard protocol. Tissue homogenates were treated with 0.26 Wunsch U/ml Liberase and 10 U/ml DNase (Sigma-Aldrich, St. Louis, MO, USA) for up to 1 h as needed. Single-cell RNA sequencing was performed using the Chromium Single-Cell v3 3′ Chemistry Library, Gel Bead, Multiplex and Chip Kits (10x Genomics) according to the manufacturer's protocol.