GSM7787363: Kc167, CP190, ChIP in control, bio rep1; Drosophila melanogaster; ChIP-Seq
2–3×107 cells were fixed by adding 1% formaldehyde directly to cells in culture medium for 10 min at RT with gentle agitation. 0.125 M glycine was used to quench formaldehyde with gentle agitation for 5 min. Cells were centrifugated at 500 x g for 5 min and washed twice with ice-cold PBS. Chromatin preparation and chromatin immunoprecipitation (ChIP) were performed following earlier described methods (Bag et al., 2019). Libraries were prepared according to the manufacturer's protocol (NEB; Ultra II for DNA Library Prep Kit; E7645).