GSM7026654: Spinal cord microglia from NTg animals (Sc7); Mus musculus; RNA-Seq
Total RNA was isolated using QIAzol Lysis reagent (Qiagen) and miRNeasy mini kit (Qiagen) with DNA digestion on-column, according to the manufacturer's instructions. RNA concentration and integrity was validated using the RNA 6000 Nano Kit on a 2100 Bioanalyser (Agilent) Samples were rRNA depleted and prepared for sequencing using SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara) Library size and purity control was performed on a 2100 Bioanalyzer (Agilent) using a High-Sensitivity DNA-Chip. Library concentration was measured using a KAPA Library Quantification Kit.