GSM8110786: Control mice1; Mus musculus; OTHER
Samples were prepared by embedding fresh tissues in OCT Compound (PN-4583, Sakura), and stored at -80℃. To quality the samples, tissue sections were cryosectioned at 10μm by Leica CM1950 cryostat.Then, Hematoxylin & Eosin(H&E) staining, and imaging were performed following 10x Genomics Visium Spatial protocols (CG000160 Rev C, 10X Genomics). Also, some tissue sections were collected to isolate RNA with miRNeasy Mini Kit (PN-217004, QIAGEN).After quality, tissue sections were cryosectioned at 10μm and placed within pre-equilibrated Visium Spatial slide. The libraries were prepared by Visium Spatial Gene Expression Reagent Kits (PN-1000184, 10x Genomics) according to the manufacturer's protocol.