GSM7911481: Mouse, regular chow diet, control 8; Mus musculus; ncRNA-Seq
RNA was extracted using the miRNeasy Mini Kit (Qiagen, 217004) according to the manufacturer's protocol, followed by RNA concentration determination (NanoDrop 2000, Thermo Scientific), standard gel electrophoresis for quality assesement, and RIN determination (Bioanalyzer 6000, Agilent). RIN was determined to be above 8.4 for all samples. Libraries were constructed from 200ng total RNA (NEBNext Multiplex Small RNA library prep set for Illumina, New England Biolabs, NEB-E7560S) and the small RNA fraction was sequenced on the NextSeq 500 System (Illumina) at the Center for Genomic Technologies Facility, the Hebrew University of Jerusalem NEBNext Multiplex Small RNA library prep set for Illumina, New England Biolabs, NEB-E7560S