GSM8193305: OME 11 + FMT; mouse gut metagenome; OTHER
Murine fecal samples were collected for bacterial genomic DNA extraction using the centrifugal affinity column system in the PureLink Microbiome DNA Purificaction Kit (Invitrogen), according to the manufacturer's instructions. Quantity and purity of the extracted genomic DNA were evaluated with a Thermo Scientific NanoDrop and stored at -20ºC until used. For long-read sequencing of 16S rRNA gene, 10ng of total genomic DNA were subjected to library construction using the 16S barcoding kit (SQK-16S024) according to Oxford Nanopore Technologies (ONT) protocol. Specifically, bacterial DNA was amplified using barcoded universal 16S primers (27F and 1492R) supplied by ONT with 5'-tags that facilitate the ligase-free attachment of rapid sequencing adapters required during nanopore library preparation. The barcoded libraries were loaded and sequenced on MinION flow cells (FLO-MIN106D R9.4.1; ONT) using the MinION-Mk1C instrument (ONT)