GSM8194107: DMSO-2; Homo sapiens; RNA-Seq
To extract total RNA, we utilized the MJzol Animal RNA Isolation Kit (Majorivd) following the standard protocol provided by the manufacturer. Subsequently, we purified the RNA using the RNAClean XP Kit (Beckman Coulter) and RNase-Free DNase Set (QIAGEN). The integrity of the RNA was assessed with the Agilent 2100 Bioanalyzer/Agilent 4200 TapeStation (Agilent technologies), while the total quantity and purity of RNA were measured using the Qubit 2.0 Fluorometer (Thermo Fisher Scientific) and NanoDrop ND-2000 Spectrophotometer (Thermo Fisher Scientific). Following RNA purification, the Total RNA is further processed for mRNA separation, fragmentation, first-strand cDNA synthesis, second-strand cDNA synthesis, end repair, 3' end A-tailing, adapter ligation, and enrichment to complete the construction of the mRNA sequencing library. Subsequently, the library concentration is determined using the Qubit 2.0 Fluorometer (Thermo Fisher Scientific), and the fragment distribution is analyzed with the Agilent 4200 TapeStation (Agilent technologies).