GSM6601461: PM2.5-treated worms in day 5; Caenorhabditis elegans; RNA-Seq
Total RNA was extracted using Trizol reagent. A total amount of 3 µg RNA per sample which passed through the quality test was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample. The library quality was assessed on the Aillient Bioanalyzer 2100 system.