GSM7882148: 10T, DNMT3A-WT, IgG, Cut&Run, R1; Mus musculus; OTHER
CUT&RUN reaction: 500K cells were dispensed to individual wells of a 96-well plate, immobilized onto Concanavalin-A beads, and incubated overnight (4°C) with 0.5 µg of antibody. pAG-MNase was added and activated (2 hours @ 4°C) and CUT&RUN enriched DNA purified using Serapure beads. Recovered DNA was quantified using PicoGreen and reactions were normalized to 5ng DNA (or entirety of the reaction if <5ng DNA was recovered) before preparing sequencing libraries (CUTANA CUT&RUN Library Prep kit; EpiCypher #14-1001)