GSM6601122: SH-SY5Y WT 2; Homo sapiens; RNA-Seq
RNA was extracted using the QIAGEN RNeasy kits following manufacturer's protocol. RNA was quantified using a Nanodrop spectophotometer and assessed for quality by measuring the 28S/18S ratio by agarose gel electrophoresis. Library construction was performed by Eurofins Genomics under their NGSelect RNA on Illumina. The library construction involved purification of poly-A containing mRNA molecules, mRNA fragmentation, random pried cDNA synthesis, adaptor ligation and adapter specific PCR amplification.