GSM8147816: WT3; Mus musculus; RNA-Seq
Total RNAs were extracted using TRIzol reagent(Invitrogen, cat. NO 15596026) following the methods by Chomczynski et al (DOI:10.1006/abio.1987.9999). 2 μg total RNAs were used for stranded RNA sequencing library preparation KC-DigitalTM Stranded mRNA Library Prep Kit for Illumina® (Catalog NO. DR08502, Wuhan Seqhealth Co., Ltd. China) following the manufacturer's instruction.