NextSeq 500 sequencing; RNA-seq of granulosa-like cells derived from human embryonic stem cell lines from different parental backgrounds
The creation of CSES7 cell line is described in Lavon et al. (2008) Derivation of euploid human embryonic stem cells from aneuploid embryos. Stem Cells 26: 1874-1882. CSES10 in Narwani et al. (2010) Human embryonic stem cells from aneuploid blastocysts identified by pre-implantation genetic screening. In Vitro Cell Dev Biol Anim. 46: 309-316. The PES6 and SWAPS4 cell lines are described in Paull et al. (2013) Nuclear genome transfer in human oocytes eliminates mitochondrial DNA variants. Nature: 493 632–637. Cells washed and trypsinized Total RNA was isolated using RNeasy Mini Kit (QIAGEN) RNA sequencing libraries were generated using TruSeq RNA Library Prep Kit (Illumina) according to the manufacturer's protocol and sequenced using Illumina NextSeq 500 with 75 bp single end reads