GSM7680535: Non-native rep_1; Erigeron canadensis; Bisulfite-Seq
Fresh leaf samples were collected from both native and non-native sites of Conyza canadensis. The young leaves collected were immediately frozen using liguid nitrogen and stored in -80 degrees till DNA extraction. Total DNA was extracted from both native and non-native leaf samples using plant DNA extraction kit (QIAGEN) and 10μg of DNA from all the four samples was send for whole genome bisulfite sequencing. BS-Seq library construction and sequencing was performed on both native and non-native samples following manufacturers protocol.