GSM7595574: Syngeneic_mouse_lung, 2hrs_post_reperfusion, RvD1_donor, scRNAseq; Mus musculus; RNA-Seq
Two hours after lung transplantation, left lung grafts from two mice were collected and pooled for submission of each sample. Lung tissue was digested and single cell suspensions were prepared as previously described [PMID 28615357]. Single-cell suspensions were submitted to the Genome Technology Access Center core facility (Washington University in St. Louis) for single-cell GEM construction and cDNA synthesis and library construction. Samples were processed using the Chromium Single Cell 3' Library & Gel Bead Kit (10X Genomics, v3) following the manufacterer's protocol. The libraries were sequenced on NovaSeq S4 (Illumina) targeting 50,000 reads per cell and 500 million read pairs per library.