GSM6434403: Donor1_1st-stimulated_AAVS1_1; Homo sapiens; RNA-Seq
On days 0, 5 and 20 of the CAR T-cell restimulation assay, before stimulation and after the fourth tumor challenge respectively, CD8+ CAR T-cells were isolated using CD8 microbeads (Miltenyi Biotec) and total mRNA was extracted with RNA Clean & Concentrator™ kits (Zymo Research). Bulk RNA-seq was conducted by Novogene using the Novaseq6000 system (paired-end 150bp) at 40 × 106 reads per sample.