GSM7785552: GM12878_dTag_multiPlex2_scATAC; Homo sapiens; ATAC-seq
Nuclei were extracted using NIB-Hepes buffer Nuclei for scATAC and dTag were carried through tagmentation, dTag nuclei or nuclei for scWGS alone were carried through nucleosome disruption followed by tagmentation. dTag amnd multiplexed scWGS samples used indexed tagmentation using reagents from the ScaleBio scATAC multiplexing kit. Single-plex experiments used the tagmnetaiton enzyme provided in the 10x Genomics scATAC kit. After final tagmentation, nuclei were carried through the 10x scATAC v2 protocol according to the default protocol or using the modifications indicated by ScaleBio for samples that leevraged indexed tagmentation.