GSM8244833: L4,scRNAseq, CRISPR; Homo sapiens; RNA-Seq
Equal numbers of double-positive cells from each donor were pooled and prepared for single cell RNA-sequencing, with 4 GEM groups each loaded with 20,000 cells, using the Chromium Next GEM Single Cell 3' Kit 3.1 (10x Genomics, 1000268) according to the 10x Genomics protocol for Feature Barcode technology for CRISPR Screening. Libraries were prepared with the Chromium Next GEM Single Cell 3' Kit 3.1 (10x Genomics, 1000268) according to the 10x Genomics protocol for Feature Barcode technology for CRISPR Screening. The resulting guide and expression libraries were sequenced on a Novaseq 6000 (Illumina).