Illumina HiSeq 4000 paired end sequencing; ATAC-seq of human oesophageal adenocarcinoma OE19 cell line treated with lapatinib for 35 days followed by drug withdrawal
OE19 cells were treated with DMSO for 1 day or 500 nM lapatinib for 35 days or 500 nM lapatinib followed by lapatinib withdrawal for 1, 2, 3 or 14 days. Cells were collected by trypsinisation. Omni ATAC-seq protocol:Corces, M., Trevino, A., Hamilton, E. et al. An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues. Nat Methods 14, 959–962 (2017). https://doi.org/10.1038/nmeth.4396 Omni ATAC-seq protocol:Corces, M., Trevino, A., Hamilton, E. et al. An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues. Nat Methods 14, 959–962 (2017). https://doi.org/10.1038/nmeth.4396