GSM6404207: MDA-MB-231 cells pre-treated with nutrition medium mixed with DMEM (1:1) for 3 days_rep 2; Homo sapiens; RNA-Seq
Cells were lysed by TRIzol reagent, and total RNA was extracted according to the manufacturer's instructions. 1 µg of total RNA per sample was used to generate a sequencing library using KAPA mRNA HyperPrep Kit. mRNA was purified from the total RNA using magnetic oligo-dT beads and fragmented under high-temperature incubation. Double-stranded cDNA was then synthesized with the additional dAMPs on 3' ends, and the adapters with 3' dTMPs were applied to generate the final library fragments. Library fragments ranging from 300-400 bp were purified with the KAPA Pure Beads system, and the library was amplified using KAPA HiFi HotStart ReadyMix. The library PCR products were purified using the KAPA Pure Beads system and the quality was assessed by the Qsep 100 platform.