GSM8353776: scKPC1242_FA_s2; Mus musculus; RNA-Seq
Total RNA was extracted using RNeasy mini kit (Qiagen). RNA with a RIN > 8 determined by using 2100 Bioanalyzer System with RNA 6000 Nano Kit (Agilent Technologies) was used for library preparation. 4 μg of RNA was used for the preparation. Sequencing library was prepared using TruSeq Stranded Total RNA kit (Illumina) according to the manufacturer's instructions, except 12 PCR cycles were used.