GSM7508279: Polysome_sgDap5_Act_1; Mus musculus; RNA-Seq
For total RNA, cells were lysed with TRIzol (Thermo Fischer) and RNA purified following the manufacturer's instructions). Polysomes were isolated on sucrose gradient as described previously (PMID: 28388414). Fractions 9-13 were collected for RNA isolated using TRIzol. Libraries were prepared for sequencing using standard Illumina protocols (PolyA-bead enrichment).