GSM6467247: CD19-28z CAR-T cells, day13 [sample7]; Homo sapiens; RNA-Seq
On day 9 and 13, CAR positive cells were labelled with biotin-anti-mouse IgG (Jackson Laboratory) and streptavidin-PE and sorted on FACSAriaII cell sorter. RNA was harvested using Rneasy mini kit (Qiagen). 0.4 ~ 2.1 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) following manufacturer's protocols.