GSM8193211: DPH5_H3; Thunnus thynnus; RNA-Seq
tissues were homogenized in Tri-reagent using a tissue homogenizer. Chloroform was added, and the mixture was centrifuged to separate the phases. The aqueous phase containing RNA was collected, and RNA was precipitated with isopropanol. After washing with ethanol, the RNA pellet was air-dried and dissolved in RNase-free water. RNA quality, quantity and integrity of the isolated RNA were assessed using a NanoDrop spectrophotometer and an Agilent Bioanalyzer. cDNA libraries for NGS were prepared using the TruSeq RNA Library Preparation Kit (Illumina).