GSM8245470: SUM159_spheroid; Homo sapiens; RNA-Seq
The different cell populations were labelled with the BD Single-Cell Multiplexing Kit (BD Biosciences),  following the manufacturer's protocol. Cell viability and numbers were  evaluated by the BD Rhapsody scanner, and samples with high viability (> 85%) were used for sequencing. Approximately 15000 cells for the attached SUM159 were pooled together and another 15000 cells for the speroid and loaded in 2 BD Rhapsody cartridges. The BD Rhapsody protocol was followed for library construction