GSM7595872: HD-SB; Homo sapiens; RNA-Seq
Total RNA was extracted by use of TRIzol reagent (TR118-500, MRC, USA) RiboMinus™ Eukaryote System v2 kit was used to remove rRNA from total RNA, and RNase R was used to digest linear RNA to enrich circRNAs. RNA was fragmented for circRNAs library construction using the TrueLib mRNA Library Prep Kit for Illumina. The RNA was reverse transcribed to synthesize a two-stranded product, repaired at its end with a plus dA tail, and ligated to Adapter. The enriched product was amplified by pcr reaction. After that, the fragments were screened and purified to obtain the desired libraries.