GSM7679968: mor28iCre-tdTom OMPtTA-tetOP2, GFP+, HiC, rep1; Mus musculus; Hi-C
MOE were dissociated using the Worthington Papain Dissociation kit, and cells were fixed in 1% formaldehyde in 1x PBS, and quenched with 1/10th vol. 1.25M Glycine, and washed with 1x PBS either immediately before or after cell sorting. Nuclei were processed for HiC exactly as described in "LHX2- and LDB1-mediated trans interactions regulate olfactory receptor choice" (Horta et al. 2019). Briefly nuclei were lysed, permeabilized, quenched, and then digested over night in DpnII . The next day we performed biotin-fill in with biotinylated dUTP, ligation with T4, and shearing. Cross-links were reversed overnight and DNA was purified with a 2x AMPure bead purification. Library prep was performed on MyOne Streptavidin beads to purify biotinylated contacts. Libraries were prepared using the Ovation® Ultralow V2 DNA-Seq Library Preparation Kit.