GSM7849714: Panc-Smad4OFF_m2_multiome_ATAC; Mus musculus; ATAC-seq
Single Cell Multiome ATAC + Gene Expression was performed with 10X Genomics system using Chromium Next GEM Single Cell Multiome Reagent Kit A (catalog #1000282) and ATAC Kit A (catalog #1000280) following Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Kits User Guide and demonstrated protocol, Nuclei Isolation for Single Cell Multiome ATAC + Gene Expression Sequencing. Briefly, cells (viability 95%) were lysed for 4 min and resuspended in Diluted Nuclei Buffer (10X Genomics #PN-2000207). Lysis efficiency and nuclei concentration was evaluated on Countess II automatic cell counter by trypan blue and DAPI staining. 11,000 nuclei were loaded per transposition reaction, targeting recovery of 7,000 nuclei after sequencing. After transposition reaction, nuclei were encapsulated and barcoded. Next-generation sequencing libraries were constructed following Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Kits User Guide and sequenced on an Illumina NovaSeq 6000 system.