GSM8245644: Mouse B, ndLN, Day 8 IP, EYFP+; Mus musculus; OTHER
Freshly collected cells were sorted into Buffer RLT Plus buffer (Qiagen) with 20mM DTT. Lysate was vortexed for 1 min and stored at −80 °C for subsequent analysis. Total RNA was then extracted with RNeasy Plus Micro Kit as per manufacturers recommendations. RNA QC with Tapestation High Sensitivity RNA showed RIN values above 9.8 for all samples. Samples were prepared withTakara SMARTer Mouse TCR alpha-beta Profiling Kit for 96 samples as per manufacturers recommendations.