GSM8192883: Neuron, 18weeks, 2div, rep222; Sus scrofa; RNA-Seq
Given the large size of pig DRG neurons, the sample collection followed a procedure using two separate pipettes as follows: First, the recording pipette was retracted under slightly negative pressure from the cell and immutably broken into in PCR-tube containing 4 µl of lysis buffer (40 mM Guanidine hydrochloride, 0.1 mM Smart dT30VN primer, 5 mM dNTPs), secondly a second bigger pipette with diameter customized for cell diameter of cell to collect (resistance ~0.7 Mohm, size adapted for patched cell for ideal harvesting result) was used to collect the entire cell. After collection, this pipette was broken into the same PCR-tube. Samples were immediately frozen on dry ice. Individual lysed cells were treated according to the Smart-Seq2 library preparation protocol