GSM8013649: 1-son; Homo sapiens; RNA-Seq
PBMCs from the son (PT) and mother (MOM) were purified by Ficoll and suspend the cells with ice-cold PBS+10%FBS+2mM buffer. Single-cell RNA-seq libraries were prepared following the protocol provided by 10X Genomics Chromium Single Cell Immune Profiling Solution. Libraries were constructed with a Single Cell 3′ Reagent kit (v3). In each lane of a 10X Chromium instrument, Ficoll-purified PBMCs from the patient (PT) and mother (MOM) were encapsulated and single-cell libraries were sequenced on Illumina Hiseq X10 platform.