GSM8244414: FLC sample 8, normal; Homo sapiens; RNA-Seq
Total RNA is extracted from the samples via Rneasy kit from Qiagen. Library construction followed standard Novogene wokflow. Following cDNA synthesis, second-strand synthesis is initiated by adding second-strand synthesis buffer, deoxynucleotides, ribonuclease H, and DNA polymerase I. The process continues with terminal repair, A-ligation, and sequencing adaptor ligation. The double-stranded cDNA library is then completed through size selection and PCR enrichment.