GSM8322933: Sperm_RNA_Rep1; Mus musculus; RNA-Seq
Total RNA was isolated Qiagen using miRNeasy Mini Kit according to manufacturer's protocol. RNA sequencing libraries were prepared with Illumina-compatible NEBNext® UltraTM II Directional RNA Library Prep Kit (New England BioLabs, MA, USA), according to manufacturer's instructions, at Genotypic Technology Pvt. Ltd., Bangalore, India. Small RNA sequencing (SmRNA) library for oocyte and sperm was prepared using the NEXTflexTM Small RNA-Seq Kit v3 (Bioo Scientfic Corporation, Austin, Texas, U.S.A.), at Genotypic Technology Pvt. Ltd., Bangalore, India, following manufacturer's instructions. Small RNA sequencing (smRNA) libraries for zygote was prepared with QIAseq® miRNA Library Kit (Cat: 331502) protocol (Qiagen, Maryland, U.S.A.) at Genotypic Technology Pvt. Ltd., Bangalore, India.