GSM8148877: Control_A_M2; Homo sapiens; RNA-Seq
At the end of differentiation, 500.000 monocytes or macropohages were then stored in TriPure until further use. RNA isolation was done using the TriPure kit according to the manufacturer. Library preparation was performed using the Illumina TruSeq stranded mRNA kit with a starting input of 300ng and libraries were sequenced on the IlluminaNovaSeq 6000 instrument generating an average of 20 million reads per sample.