GSM7026597: A18 ATXN2-ALS iPSC-derived motor neurons; Homo sapiens; ssRNA-seq
Total RNA was isolated and extracted using the RNeasy Micro Kit (Qiagen with DNA digestion on-column, according to the manufacturer's instructions. RNA integrity (RIN) was verified in an Agilent 2100 bioanalyzer and only samples with RIN ≥ 8.0 were used for RNA sequencing. rRNA was depleted using the Ribo-Zero Magnetic Kit before library preparation. Stranded, paired-end sequencing libraries were prepared (Genome Analysis Facility, Groningen, The Netherlands) and sequenced in the Illumina HiSeq2500 platform (Illumina, San Diego, California).