GSM8286491: L. albus 20 min 10 mM sucrose  replicate 3; Lupinus albus; RNA-Seq
RNA from white lupin samples was isolated following the protocol for “Purification of Total RNA from Plant Cells and Tissues, and Filamentous fungi” from RNasy Plant Mini kit (Qiagen). The Qubit 4 Fluorometer in conjunction with an RNA-High Sensitivity assay was used to quantify the samples. In addition, the RNA IQ assay was used to determine the RNA integrity number (RIN). This RIN is based on the ratio of large and/or structured RNA to small RNA in the sample. Only samples with RIN of 8 or higher were used for RNA sequencing. Using the PCR-cDNA sequencing-barcoding (SQK-PCB111) kit of Oxford Nanopore Technologies, the extracted RNA was converted into cDNA and uniquely barcoded, following the manufacturer's instructions.