GSM8323361: Th17, H2Bub1, rep3, CUT&RUN; Mus musculus; OTHER
Nuclei were prepared from  in-vitro-differentiated Th17 cells using  nuclear extraction buffer (20 mM HEPES pH 7.9, 10 mM KCl, 0.1% Triton X-100, 20% Glycerol, 0.5 mM Spermidine, 1x Roche cOmplete™, Mini, EDTA-free Protease Inhibitor) CUT&RUN libraries were prepared using Epicypher's CUTANA™ ChIC/CUT&RUN Kit (#141-048) and NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7645S) indexed using NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs) (NEB #E6440S).