GSM8149006: Bulk, APP, 6am, rep1; Mus musculus; RNA-Seq
Astrocyte- or microglia-specific RNA were isolated by incubating tissue with anti-GFP or anti-HA coated beads, respectively.  RNA was then purified using the RNA Clean and Concentrator kit (Zymo Research) Total RNA integrity was determined using Agilent Bioanalyzer or 4200 Tapestation. Library preparation was performed with 10ng of total RNA with a Bioanalyzer RIN score greater than 8.0. ds-cDNA was prepared using the SMARTer Ultra Low RNA kit for Illumina Sequencing (Takara-Clontech) per manufacturer's protocol.