GSM7060654: LCMV-WE, replicate 1, scATAC-seq; Mus musculus; ATAC-seq
C57Bl6 mice were infected with LCMV-WE or LCMV-Docile. 28 days after infection, spleens were surgically removed from culled mice and non-RBC splenocytes were isolated. LCMV-specific B cells were magnetically enriched using an LCMV-NP tetramer system. Enriched cells were then stained with viability dye (FVS) and relevant antibodies, and 100,000 LCMV-specific B cells per sample were sorted using the BD Influx (BD Biosciences) cell sorter. Nuclei isolation, barcoding and library preparation were performed according to the manufacturer's protocol using the Chromium Next GEM Single Cell ATAC Reagent Kit v1.1 (10X Genomics). Libraries were prepared using the MGIEasy v3 chemistry kit and sequenced using the MGITech MGISEQ2000RS sequencer (MGI Tech).