GSM8194327: SUM159PT, ZSWIM8 KD, input, replicate 2; Homo sapiens; OTHER
Cell pellets were then lysed with 1 mL of eCLIP lysis mix and sonicated (QSonica Q800R2) for 5 minutes, 30 seconds on / 30 seconds off with an energy setting of 75% amplitude, followed by digestion with RNase-I (Ambion). The standard eCLIP protocol [PMID 27018577] was modified to enable chimeric ligation of miRNA and mRNA according to bioRxiv preprint by Manakov et al, 2022 [10.1101/2022.02.13.480296].