GSM8245203: PIEZO2 positive fibroblast; Homo sapiens; RNA-Seq
Keroid tissues were dissociated in a gentleMACS C-tube (Miltenyi Biotec) with digestion buffer composed of DMEM (Gibco) and Multi Tissue Dissociation Kit 1 using a gentle MACS Octo dissociator (Miltenyi Biotec). After running the program “37°C_multi_H”, cell suspensions were passed through a 70-μm nylon mesh sheet. Total cellular RNA was extracted with TRIzol reagent (Invitrogen). For cDNA library construction, we used a SMARTer Stranded Total RNA-Seq Kit v2-Pico Input Mammalian (TaKaRa, Shiga, Japan) following the manufacturer's protocol.