GSM6601690: Tet2mut, RNA, sample2; Mus musculus; RNA-Seq
RNA was harvested using Rneasy mini plus kit (Qiagen). 1 µg of total RNA was used for construction of sequencing libraries. gDNA for ATAC-Seq was harvested from 50.000 cells with Nextera DNA Library Preparation Kit (Illumina). RNA libraries were constructed using QUANTseq 3' mRNA-Seq Library Prep Kit (Lexogen). ATAC libraries were generated at the Biomedical Sequencing Facility of CeMM, using custom Nextera Library Adaptors.