GSM8014780: CC011_MAIT; Mus musculus; RNA-Seq
Spleen and Colon tissues were stained with MR1-5-OP-RU-APC tetramer, in the spleen enrichment with APC-magnetic beads from single cell suspensions was proformed. MAIT cells (Live Tetramer+ TCRb+) cells from each animal and tissue were simultaneously labeled with the cell surface sorting panel an oligonucleotide-tagged antibody sample tag (BD Biosciences) prior to cell sorting on a FACSAria3 (BD Biosciences). BD Rhapsody mRNA whole, WTA Kit with sample tags