GSM7849739: ATAC-Seq MDA-MB-231 shKDM2B 2; Homo sapiens; ATAC-seq
Samples were prepared using the ATAC-Seq kit (Active Motif, Cat 53150). For library preparation, tagmented DNA was amplified, using unique combinations of i7/i5 indexed primers, and on Illumina's Nextera adapters. The final step in library purification was a clean-up with SPRI beads (Beckman Coulter, Cat. B23317). The quality of the libraries was confirmed using the Bioanalyzer high sensitivity DNA assay (Agilent Technologies). Libraries were prepared from three biological replicates.