GSM7060380: Snu449; Homo sapiens; RNA-Seq
Following the manufacturer's instructions, total RNA was extracted using the RNeasy Micro Kit (Cat# 74004, Qiagen), and an Agilent 2100 Bioanalyzer was used to check for a RIN number to inspect RNA integrity (Agilent technologies, Santa Clara, CA, US). The RNAClean XP Kit (Cat A63987, Beckman Coulter, Inc., Kraemer Boulevard Brea, CA, USA) and RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH, Germany) were used to further purify the qualified total RNA.