GSM7750050: SK-N-BE(2)c xenograft, CTRL, replicate 2; Homo sapiens; RNA-Seq
Total RNA from cells was extracted using RNAqueous Micro Kit (Ambion RNAqueous™-Micro Kit, ThermoFisher, #AM1931). Total RNA from tumors was extracted using QIAshredder (Qiagen, cat. no. 79656) and RNeasy® Midi Kit (Qiagen, cat. no. 75142). The total RNA input to library preparation was 500 ng The library preparation was performed according to TruSeq® Stranded mRNA Sample Preparation Guide (Part # 15031047 Rev. E) Modification of “Enrich DNA Fragment” step: from 15 to 13 PCR cycles.