GSM6523952: Wild type replicate 1; Staphylococcus aureus; RNA-Seq
Cells were lysed by bead beating (Precellys 24 instrument - 6000 rpm, 1 min, 100 um zirconium beads) and cell lysates were clarified by centrifugation (20,000xg/10 min/4°C). Subsequently, 700 µl of supernatant was removed and mixed with 700 µl of ethanol. RNA was extracted using a Direct-Zol RNA miniprep plus kit (ZymoResearch) according to manufactures instructions, including the on column DNase I treatment step. The libraries were sequenced on the Illumina HiSeq platform for 50 bp single end reads.