GSM7507104: SLE_215_Pop5; Homo sapiens; RNA-Seq
Populations including Pop 2 (CD19+ CD138-), Pop 3 (CD19+ CD138+), Pop 5 (CD19- CD138+), and naïve B cells (CD19+ IgD+ CD27-), isolated from active SLE patients or influenza-vaccinated healthy subjects (vHC)were sorted directly into lysis buffer. RNA was isolated using the AllPrep DNA/RNA Mini kit (Qiagen) according to the manufacturer's instruction. Total RNA (50pg) was used as input for the ten cycles of PCR amplification by using SMART-seq v3 cDNA synthesis kit (Takara). Libraries were quantified by qPCR and size distribution was examined by Bioanalyer before pooling and sequencing