GSM7732041: Non-treated bshC:Tn cells replicate 1; Staphylococcus aureus; RNA-Seq
Total RNA was extracted using the RNeasy Mini Kit (Qiagen), according to manufacturer instructions with the following exception: samples were lysed by bead-beating using a BeadBlaster 24 (Benchmark Scientific). To remove ribosomal RNAs, high-quality total RNA was processed using Illumina's Ribo-Zero Bacteria kit (Illumina, San Diego, CA). cDNA libraries were generated with rRNA depleted mRNA using the KAPA RNA Hyper Plus Kit (Roche, Indianapolis, IN) following the manufacturer's instructions