GSM7732222: HEK293T cell model, 54C; Homo sapiens; RNA-Seq
RNA was extracted with RNeasy Mini Kit (QIAstock, QIAGEN AG). RNA quality was assessed on a Fragment Analyzer (Agilent Technologies) RNA-seq libraries were prepared from 500 ng of total RNA with the Illumina TruSeq Stranded mRNA reagents (Illumina) using a unique dual indexing strategy, and following the official protocol automated on a Sciclone liquid handling robot (PerkinElmer). Libraries were quantified by a fluorometric method (QubIT, Life Technologies) and their quality assessed on a Fragment Analyzer (Agilent Technologies).