GSM7785570: mESC WT Replicate 1; Mus musculus; RNA-Seq
Total RNA from three replicates of WT and Srrm2 heterozygous knockout cells was isolated with TRIzol (Invitrogen) and DNA was degraded with TURBO DNase (Invitrogen), according to the manufacturer's protocol. RNA spike-ins (Invitrogen) were added to each purified RNA sample. Total RNA libraries were produced from 600 ng of RNA using the TruSeq Stranded total RNA library preparation kit (Illumina) according to the manufacturer's protocol. Total RNA libraries were paired-end sequenced using the NextSeq 500/550 system at 75 bp length.