GSM7881658: Standard proton-irradiated M1 macrophage_3 [ST-M1-0512]; Mus musculus; RNA-Seq
Treated mouse macrophages and were lysed in TRIzol (Thermo Fisher), and RNA was extracted according to the manufacturer's instructions, followed by RNA purification using a RNeasy Plus Mini Kit (Qiagen). The RNA from each step and library DNA quality were analyzed with RNA Nano assay chips, RNA Pico assay chips, and DNA Nano assay chips using a 2100 bioanalyzer (Agilent). Library was subjected to next-generation sequencing analysis in a high-throughput sequencing center with a NovaSeq at the Azenta Life Sciences