GSM8148845: Ngn2 iNeurons isogenic CTL isoCTL 3; Homo sapiens; RNA-Seq
RNA was isolated using the RNeasy mini Kit (QIAGEN) and genomic DNA was removed using the RNase-Free DNase Set (QIAGEN). Retrotranscribed cDNA was obtained from 1 μg of total RNA using the SuperScript VILO Retrotranscription kit from Life Technologies according to the manufacturer's instructions. Library preparation for RNA-seq was done with the Ribo-Zero Total RNA sample preparation kit (Illumina), starting from 250 ng to 1 g of total RNA. The quality of cDNA libraries was assessed by Agilent 2100 Bioanalyzer using the High Sensitivity DNA Kit.