GSM8148858: Pan02 tumor, PBS; Mus musculus; RNA-Seq
On day 14 post-tumor-inoculation, mice were euthanized, and the tumors were isolated. Tumors were cut into 2–4 mm pieces and placed into a gentleMACS C tube (Miltenyi). Tumors were enzymatically digested using the gentleMACSTM Dissociator and tumor Dissociation kit (Miltenyi) according to manufacturer's protocol. Cells were filtered through a 70 µm cell strainer to discard any remaining undigested tissue before proceeding with the FACS staining. Zombie Aqua Viability dye (Zombie dye, BioLegend) and PE-Cy7 anti-CD45.2 (clone 104, Biologend) were used to sort for live immune cells. Single Cell 3′ v3 performed on a Chromium Controller