GSM8193907: FXS2; Mus musculus; RNA-Seq
Freshly dissected mouse cortex in cold physiological buffer, then homogenized to single nuclei and sorted for astrocytes by SOX9 (Abcam AB185966) and NEUN (Millipore #MAB377X) immunostaining as in the protocol from Labarta-Bajo et al., 2023. Sorted SOX9+NEUN- nuclei were lysed, and all RNA extracted with Qiagen RNeasy Micro kit with DNase step. Illumina Ribo-Zero Plus rRNA Depletion Kit with IDT for Illumina RNA UD Indexes, following manufacturer instructions