GSM8014679: Control repl. 2; Mus musculus; RNA-Seq
Total RNA was isolated by crushing the femur with an Ultraturrax in Trizol, phase separation was induced through mixing and centrifugation with chlorophorm, and the aqueous phase together with ethanol were transferred to the Qiagen Rneasy Kit. We followed the instructions of the manufacturer. libraries were prepared using the TruSeq Stranded mRNA Kit following the manufacturer's protocol