GSM7750226: Fibroblasts, Heat_Inactivated, 10hpi, rep1; Homo sapiens; RNA-Seq
At the time of cell harvest, media was removed, and the cells were washed twice with PBS and then lysed by the addition of lysis buffer (Macherey-Nagel, Nucleospin Viral RNA kit). The samples were stored at -80°C until RNA extraction according to the instructions from the manufacturer. For each biological replicate, three wells with cells were pooled. Library generation was performed by Novogene using NEB Next® Ultra RNA Library Prep Kit for Illumina® following manufacturer's protocols.