GSM8111848: EpiLC_0h_clone4_ATAC-seq; Mus musculus; ATAC-seq
50,000 cells were collected in a 1.5 ml DNA LoBind tube (Eppendorf, 0030108051), and lysed for 3 minutes on ice in 0.1% IGEPAL, 0.1% Tween-20, 0.01% Digitonin (Promega, G9441). Nuclei were washed and pelleted for 10 minutes at 500g, and then treated for 30 minutes at 37ºC with Tn5 transposase (Illumina, 20034197). DNA was extracted using DNA Clean and Concentrator 5-kit (Zymo Research, D4014), and libraries were prepared according to Buenrostro et al. 2013.