GSM7910776: Patient #159, human colon organoids, differentiation (B+D) medium - control; Homo sapiens; RNA-Seq
Macherey-Nagel RNA extraction Kit was used in combination with TRIZOL. Both large and small RNA was extracted. 500 ng of total RNA was used for the construction of sequencing libraries RNA samples (500 ng; RQS/RIN average 8.6, range 7.3-9.6) were converted into cDNA sequencing libraries with the "QuantSeq 3' mRNA-Seq Library Prep Kit (FWD) for Illumina" (Lexogen, Cat.No. 015). Briefly, library generation is initiated by reverse transcription with oligodT priming, followed by a random-primed second strand synthesis.