GSM8244641: PT id9 tumor tissue; Homo sapiens; RNA-Seq
Tumor and non-tumorous cells were collected from the sections using laser microdissection (LMD 7000, Leica, Benshein, Germany). Total RNAs were extracted using RNeasy Micro Kit (Qiagen, Hilden, Germany). RNA-seq libraries were prepared with 100 ng of total RNA using an Ion AmpliSeq Transcriptome Human Gene Expression kit (Thermo Fisher Scientific, Waltham, MA) according to manufacturer's instructions. The generated cDNAs were subjected to multiplex amplification with Ion AmpliSeq Transcriptome Human Gene Expression Core Panel containing 18,574 protein-coding RNAs and 2,228 non-coding RNAs. The libraries were sequenced with 100 bp single-end reads on Ion Proton using an Ion PI Sequencing 200 kit v3 and Ion PI Chip v2 (Thermo Fisher Scientific).