GSM8286600: lpwRCC2971, LP-WGS; Homo sapiens; OTHER
Tissue was fixed by 2mmol/L disuccinimidyl glutarate (DSG) for 10 minutes followed by 1% formaldehyde for 10 minutes, and quenched with glycine. Chromatin was sheared using the Covaris E220Ultrasonicator. ChIP-seq libraries were generated from purified input and IP sample DNA using the ThruPLEX DNA-seq Kit from TakaraBio. Libraries were sequenced using 150-base paired-end reads on an Illuminaplatform.