GSM7142962: Resting-MCM, replicate 2; Mus musculus; RNA-Seq
Resting-MCM, LPS-MCM, and Hemin-MCM treated astrocytes were harvested, and total RNA was isolated using TRIzol (Invitrogen). RNA integrity was evaluated using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The libraries were constructed using TruSeq Stranded mRNA LTSample Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer's instructions.