GSM7429882: young_shoots_Col-0_ATAC_seq_rep2; Arabidopsis thaliana; ATAC-seq
Approximately 1 g of tissue was used for each ATAC-seq assay. The samples were chopped with a razor blade in 1×PBS buffer to obtain the intact nuclei. After filtration through Miracloth, Triton X-100 was added and the samples were incubated for 12 min on ice. The nuclei were pelleted by centrifugation and were resuspended in 20 μl TTBL buffer (VAHTS, TD501). Approximately 10,000 nuclei were treated with Tn5 (VAHTS, TD501) at 37 °C for 30 min. Then, the sample was immediately purified using a Qiagen MinElute kit and the purified fragments were amplified for 7-10 cycles to construct a library, according to the instructions (VAHTS, TD501).