GSM8015861: CLKGFP, D10, ZT12, IgG, CUT&RUN, Rep 2; Drosophila melanogaster; OTHER
Photoreceptor nuclei were immunoenriched using the nuclei immunoenrichment protocol (dx.doi.org/10.17504/protocols.io.buiqnudw), followed by CUT&RUN (dx.doi.org/10.17504/protocols.io.zcpf2vn). For CUT&RUN, 1 ug of either anti-GFP antibody (Abcam ab6556), or IgG (EpiCypher CUTANA IgG Negative Control Antibody) was used.  MNase digestion was performed for 5 min using 1 ug of antibody. Libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina. Modifications to the library preperation were made to preserve short DNA fragments generated in TF CUT&RUN (dx.doi.org/10.17504/protocols.io.bagaibse). CUT&RUN