GSM7596345: WT clone2, replicate 1, day 70; Homo sapiens; RNA-Seq
Total RNA was harvested using miRNeasy mini kit (Qiagen). Approximately 100 ng of total RNA was used for the construction of sequencing libraries. RNA-seq libraries were prepared from ~400 ng of total RNA using the STRYPOLYA library prep kit (Illumina) and were sequenced at the Columbia Genome Center on a Novaseq 6000 instrument (Illumina) with 100bp paired-end reads.