GSM8244909: PF-382_ATACseq_KO_clone23_rep2; Homo sapiens; ATAC-seq
Freshly cultured cells were counted using trypan blue and a cell suspension volume corresponding to 6 million live cells was washed once with PBS (-Ca2+ -Mg2+) and then crosslinked in a volume of 5 mL PBS with 2% formaldehyde for 10 minutes at room temperature, followed by quenching with Stop Solution 1 according to the Arima protocol for HiChIP, or  Glycine Solution (10X, Cell Signaling, #7005S) according to SimpleChiP kit (Cell Signaling, #56383) for ChIP-seq. For ATAC-seq, 50,000 cells were taken from culture and washed once in cold PBS before proceeding with cell lysis, nuclei extraction, tagmentation and library preparation according to the Omni-ATAC protocol (PMID 28846090) All H3K27ac HiChIP experiments were performed using the Arima-HiC+ kit (Arima Genomics A101020) according precisely to the manufacturer's protocols (Arima-HiC+ Document numbers A160168 v00 (HiChIP) and A160169 v00 (library preparation)). All ChIPseq experiments were performed using the SimpleChiP kit (Cell Signaling, #56383) according precisely to the manufacturer's protocols.