GSM7506743: Aca2; Acinetobacter calcoaceticus; RNA-Seq
extraction of total RNA, detection of RNA integrity and total RNA content,  then remove Ribosome RNA (rRNA) from total RNA to obtain mRNA. Subsequently, a fragmentation buffer was added to randomly break the obtained mRNA into short fragments and construct the library in a chain specific manner. The cDNA libraries for RNA sequencing were constructed according to the chain specific library by the NEBNext Ultra Directional RNA Library Prep Kit for (Illumina) as per manufacturer's instructions (NEB #E7420S)