GSM7910372: HCT116 cells, poly I:C, 7h, biol rep 1; Homo sapiens; RNA-Seq
TRIzol (Ambion) was added directly to the cell pellet obtained after centrifugation at 10,000 g for 30 sec. After precipitation of the extracted nucleic acids, DNase I (TaKaRa) was treated to remove DNA, and purified RNA was reverse transcribed using RevertAid reverse transcriptase RNA libraries for RNA-seq were prepared using STruSeq Stranded Total RNA LT Sample Prep Kit (Gold) following TruSeq Stranded Total RNA Sample Prep Guide, Part # 15031048 Rev. E