GSM7789478: Cry1-/- Cry2-/- C57BL/6J Circadian Time 8 rep4; Mus musculus; RNA-Seq
Total RNA from liver samples (WT and CRYDKO mice treated with oxaliplatin at CT8 and CT16) were isolated by using RNeasy Mini Kit (QIAGEN, Germany). Quality of amount of RNA were assessed by using Qubit (Thermo Fisher Scientific, USA). High quality RNA samples (OD260/280 1.8-2.0) were used to prepare library for RNA Sequencing analysis. Briefly, adenylated mRNAs were isolated by using Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, US). Then mRNAs were fragmented, converted to cDNA and second strand synthesis was performed.