GSM7750149: MYOX_WT_2; Mus musculus; RNA-Seq
Pregnant dams were harvested, embryos were dissected, and each embryo was placed in a 1.5 mL Eppendorf tube and flash frozen in liquid nitrogen, then stored at -80C while genotyping was performed. Once embryos were generated, all samples were purified at the same time.  Each whole embryo was homogenized, and RNA was extracted by using RNeasy Micro Kit (Qiagen) using the manufacturer's protocol. RNA sequencing libraries for each sample were prepared with 1 mg total RNA by using the TruSeq RNA Sample Prep v2 Kit (Illumina) per the manufacturer's instructions, and sequencing was completed on the Illumina NovaSeq 6000.