GSM8147508: sci-Plex-EGFRi-ATCCpilot; Homo sapiens; RNA-Seq
24 hours hours after drug exposure, cells were harvested following the sci-Plex protocols (Srivatsan, McFaline-Figueroa, Ramani et al. 2020 Science) as described in the methods section of our manuscript. Nuclei were isolated in-well and nuclei barcoded by fixation of well-specific barcode containing oligonucleotides. After fixation wells were pooled and nuclei pellets flash frozen until sci-RNA-seq library preparation. Nuclei were subjected to a modified version of the three-level sci-RNA-seq described in  Cao et al. 2019 Nature 566(7745) p. 496-502 that incorporates random hexamer priming as in Sziraki et al. 2023 Nature Genetics with modifications to allow compatibility with sci-Plex multiples increase yield and decrease cost, see methods.