GSM8354739: Pre-Plasma germinal center B cells, WT1; Mus musculus; RNA-Seq
Briefly, single-cell suspensions were obatined by mincing and filtering mouse spleens and bone marrows through 70-μm cell strainers.  Cells were incubated in phosphate-buffered saline (PBS) containing 2% (w/v) FCS with indicated antibodies for 25min at 4°C. Cell sorting were performed with BD Aria II or Aria Fusion. 2 ug total RNAs were used for stranded RNA sequencing library preparation using KC-Digital(TM) Stranded mRNA Library Prep Kit for Illumina(R) (Catalog NO. DR08502, Wuhan Seqhealth Co., Ltd. China) following the manufacturer's instruction.