GSM7146910: Synovial ILCs, R5 healthy mice gene expression library; Mus musculus; RNA-Seq
Cells were sorted and purified cells were stained for 30 min on ice in PBS containing 1 % (w/v) BSA with hashtag antibodies (TotalSeq-B0301/B0302/B0303/B0304B0305 anti- mouse Hashtag 1/2/3/4/5, respectively, Biolegend). Cells were washed, counted and concentrated to 1,000 cells / µl prior to pooling. Up to 25,000 cells (hyper-loading) pooled from 4 animals were loaded into a single well of a Chromium chip G (10x Genomics). 3' gene expression libraries were generated using Chromium Next GEM Single Cell 3' Kit 3.1 with 3' Feature Barcode Kit and dual indexing (10x Genomics protocol CG000316 Rev D). Libraries were sequenced as PE150 by Illumina sequencing to 65-80% saturation by the external provider Novogene.