GSM7731036: control OL replicate 1; Mus musculus; RNA-Seq
For RNA extraction, cells were scraped with RLT buffer and RNA was extracted with the RNeasy Plus Micro kit (Qiagen, 74034). cDNA and library synthesis were done inhouse using the Smart-seq2 protocol as previously described 14 (detailed protocol at https://doi-org.laneproxy.stanford.edu/10.17504/protocols.io.2uvgew6) with several modifications. Libraries were pooled and sequenced on a Nextseq550 sequencer (Illumina) using single end 63bp for Read 1 and 12bp for index 1 with a high output 75bp kit (20024906, Illumina).  Libraries were sequenced to a depth of at least >10 million reads per sample.