GSM8194088: ADSC_Control_ATAC; Mus musculus; ATAC-seq
Nuclei were isolated before transposition. Briefly, approximately 50,000 cells were collected and rinsed with cold PBS. The cells were then suspended in 50 ul lysis buffer (10mM Tris-HCl pH 7.4, 10mM NaCl, 3 mM MgCl2, 0.2% (v/v) IGEPAL CA-630) and  incubated on ice for 10min. The nuclei were then collected by centrifugation at 500 g for 5 minutes at 4℃.ŸRŹ_x0006_ƣR_x0001__x000C_=7_x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000_ʯ_x0000_ÿATAC-seq sequencing reads were aligned to m ATAC-seq libraries was prepared using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme TD501) according to manufacturer's instructions