GSM8108155: Control_IgA_1; Homo sapiens; RNA-Seq
Cells were washed, trypsinized,follwed by total RNA was extraction using TRNzol Universal total RNA extraction kits (TIANGEN, DP424). 2ug total RNA was used for the construction of sequencing libraries. 2 μg total RNAs were used for stranded RNA sequencing library preparation using KC-DigitalTM Stranded mRNA Library Prep Kit for Illumina® (Catalog NO. DR08502) following the manufacturer's instruction. The kit eliminates duplication bias in PCR and sequencing steps, by using unique molecular identifier (UMI) of 8 random bases to label the pre-amplified cDNA molecules. The library products corresponding to 200-500 bps were enriched, quantified and finally sequenced on DNBSEQ-T7 sequencer (MGI Tech Co., Ltd. China) with PE150 model.