Illumina HiSeq 4000 paired end sequencing; RNA-seq of human oesophageal adenocarcinoma OE19 cell line following PPARGC1A knock-down and 24 hour lapatinib treatment
OE19 cells were reverse transfected with siRNAs and then 24 hours post-transfection, cells were treated with DMSO or 500 nM lapatinib for 24 hours RNA was extracted using the RNeasy Plus Kit (Qiagen) RNA-seq libraries were constructed using Illumina TruSeq Stranded mRNA kit