GSM8194320: PGCs, ND, E18.5, WGBS, rep1; Mus musculus; Bisulfite-Seq
The SSEA-1-positive female germ cells obtained through FACS were washed with PBS, and DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (QIAGEN). Bisulfite conversion was first performed. The converted DNA was then amplified by nested PCR.