GSM7507297: Trim33KO_HA_E2f4-dDB rep1; Mus musculus; OTHER
Cells were fixed with 0.2% formaldehyde and Cut&Run assay was performed using the anti-HA tag antibody (Cell Signaling #2367S) or anti-Recql antibody (Abcam ab151501) and the NEB CUT&RUN kit (#86652P). Sequencing libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina according to manufacturer's protocol