GSM7911439: Ligand_NFKB1_2; Mus musculus; OTHER
Briefly, cells were harvested, washed, and bound to activated Concanavalin A-coated magnetic beads and permeabilized. The bead-cell complex was incubated overnight with either an anti-NFKB1 antibody or an IgG antibody control at 4°C. Cells were then washed with digitonin buffer, resuspended in 50 µl of pAG/Mnase and incubated for 2h at 4°C. Enriched DNA fragments were purified using QIAGEN MinElute PCR Purification Kit. CUT&RUN libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina according to manufacturer's protocol.