GSM8147529: NB_GEX; Mus musculus; RNA-Seq
For single cell sequencing of all CD4+ T cells, sorted CD3+B220-Tcrb+CD4+ cells from 3 SPF and 3 N.b- infected mice were each labeled with TotalSeq™-C0301,C0302 and C0303 anti-mouse Hashtag antibodies (Biolegend) so that cells from individual mice had unique hashtag-oligos. Antibody cocktail was prepared according to manufacturer's protocol and labeling was performed by incubating cells with each oligo-tagged antibody for 30 minutes at 4C at 1:50 Ab dilution. Gene expression, TCR, and barcode libraries were created and sequenced using the ChromiumSingle Cell 5ʹ GEM, Library, TCR & Gel Bead Kit v2 (10x Genomics).