GSM7908905: WT_1024c_Biol_Rep_2; Danio rerio; RNA-Seq
Total RNA was extracted at the desired stages and genomic DNA removed by DNAse treatment. RNA containing s4UTP was then biotinylated and enriched with magnetic beads. Incorporated s4UTP was then chemically treated with IAA (SLAM chemistry) to introduce specific point mutatinos during retrotranscription step. 4 WT samples (one fro each stage) were not treated with IAA and used to determine SNPs. Libraries were prepared with the TruSeq stranded mRNA Kit (Illumina, #20020594) and TruSeq RNA UD Indexes (Illumina, #20022371). The polyA enrichment step was not performed. Fragmentation was performed for 1 minute at 94°C.