GSM6704288: NSC, Veh, Veh, Rep1; Mus musculus; RNA-Seq
After 48 hours, cells were harvested with Accutase (StemCell technologies) and a subset (approximately 75,000 cells) was pelleted prior to RNA extraction using the NucleoSpin RNA Plus XS kit (Takara) following manufacturer protocol. Assessment of RNA quality with Qubit RNA HS assay kit (Invitrogen) revealed RIN values of at least 8 for all samples. Libraries were generated with the Clontech SMART/Seq HT kit (Takara).