GSM7595840: Embryo L46-8, microdissected heart field at stage E8.5f, left side; Mus musculus; RNA-Seq
RNA was extracted in TRIzol-Chloroform and purified using the RNeasy micro kit (QIAGEN). RNA quality and quantity were assessed using Fragment Analyzer (Agilent Technologies). All RQNs were higher than 9.7. The library was established using the Nugen Universal Plus mRNA-Seq kit, using 15 ng of total RNA per sample. The oriented cDNAs produced from the poly-A+ fraction were PCR amplified (15-18 cycles). An equimolar pool of the final indexed RNA-Seq libraries was sequenced on an Illumina NovaSeq6000, with paired-end reads of 130 bases and a mean sequencing depth of 37,15 million reads per sample.