GSM8286257: Sample19_onChip.PBS.3; Mus musculus; RNA-Seq
RNA was harvested from each sample using a RNeasy Plus Mini kit (Qiagen) following manufacturer's protocols. Prior to extraction, all samples were placed in RLT buffer and homogenized using a Model 150 VT Ultrasonic Homogenizer at 30% power for 7 pulses over 2 min, then vortexed at maximum speed for 1 min. RNA samples were sent to NovoGene Co for sequencing, where a non-directional low-input eukaryotic mRNA library was prepared.