GSM8242575: uro_stm2457_rip2; Homo sapiens; RIP-Seq
RNA immunoprecipitation was performed using the Magna RIP RNA-binding Protein Immunoprecipitation kit (Millipore), following the manufacturer's guidelines. Preparation of whole cell lysates was first performed by a freeze-thaw cycle. Lysates were then clarified by centrifugation and subsequently incubated overnight with anti-YTHDC1 primary antibody (Proteintech) at a 1:1000 dilution. Coprecipitated RNA was isolated using phenol:chloroform:isoamyl alcohol (125:24:1). RNA libraries for RIP-seq were prepared using SmarTer Ultra Low Input RNA v4 and NEBNext ChIP-Seq kit following manufacturer's protocols.