GSM7849981: AAV6-αSMA-NC-4; Mus musculus; RNA-Seq
Illumina HiSeq2500: Poly(A) mRNAs were enriched using magnetic oligo (dT) beads (Invitrogen, Carlsbad, CA), followed by RNA-seq library preparation using the NEBNext Ultra RNA Library Prep Kit (New England Biolabs, Hitchin, UK) according to the manufacturer's instructions. DNBSEQ-T7: Oligo(dT)-attached magnetic beads were used to purify mRNA. Purified mRNA was fragmented into small pieces with fragment buffer at appropriate temperature. Then First-strand cDNA was generated using random hexamer-primed reverse transcription, followed by a second-strand cDNA synthesis and purified using AMPure XP Beads. Afterwards, A-Tailing Mix and RNA Index Adapters were added by incubating to end cDNA repair. The cDNA fragments obtained from previous steps were amplified by PCR, and products were purified by Ampure XP Bead, get the final library.