GSM8245354: BRGoe_K4ME1_Rep1; Homo sapiens; OTHER
Cut & Run was performed using the CUTANA ChIC/Cut&Run Kit (Epicypher, Cat. No. 14-1048) exactly as per manufacturer's recommendations with 0.5X106 cells per condition. Additional modifications made were mild crosslinking of cells as recommended for the BRM antibody (Epichypher Cat No. 13-2006). Libraries were constructed using NEB Ultra II DNA Library Prep Kit (NEB, Cat. No. E7465) with PCR parameters for index PCR and library amplification based on recommendations from the Epicycpher Cut&Run kit. Quality of libraries was assessed with a Tapestation high sensitivity DNA kit (Agilent Technologies) before submitting for sequencing.