GSM7911094: D2.0R cells, Athymic nude mouse, 1; Mus musculus; RNA-Seq
RNA was isolated from lung samples and 2-D culture samples using the Arcturus PicoPure RNA Isolation Kit (Thermo Fisher). Cells were snap-frozen at -80 C before RNA extraction. cDNA was prepared using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech), followed by sequencing library preparation using the Nextera XT DNA Library Preparation Kit (Illumina). Samples were then analyzed on an Illumina NovaSeq 6000.