GSM8353827: sputum, control, rep 10; Homo sapiens; RNA-Seq
Fresh sputum samples with viability >70% was prepared by suspending the cell pellet (~1 x 10^6) in 1ml PBS and processed using the Chromium V2 chips and protocol from 10X Genomics for RNA Seq. Some of the samples were preserved using DMSO and thawed before processing using the protocol. Library was constructed according to the manufacter's instructions (single cell 3' V2 protocol, 10x Genomics).