GSM8061873: Parascaris univalens, ovaries, CENP-A (345), cut-and-run, rep1; Parascaris univalens; OTHER
Parascaris tissues were frozen at -80°C and ground to a fine powder using a mortar and pestle with liquid nitrogen. CUT&RUN was done using the EpiCypher CUTANA ChIC-CUT&RUN kit (#14-1048). For each reaction, 0.5 μg of antibody against H3K9me3 (CMA318), H3K4me3 (CMA304), or CENP-A (#325 and #345) was used. Sequencing library preparation was done using a 2S Plus DNA Library Kit from Swift/IDT (# 21024).