GSM7987449: Intense and Acute screen; Mus musculus; OTHER
Genomic DNA was isolated using the Blood and Cell culture MAXI Kit, according to manufacturer's instructions. sgRNAs were amplified using a one-step barcoding PCR with NEBNext High Fidelity 2X PCR Master Mix and the forward and reverse primers. The hexa-N nucleotide stretch contains a unique barcode identifying each sample following deep sequencing.