GSM7146928: hTNFtg197 + Il23mc Fap+ synovial cells gene expression library; Mus musculus; RNA-Seq
Cells were sorted and purified cells were stained for 30 min on ice in PBS containing 1 % (w/v) BSA with hashtag antibodies (TotalSeq-B0301/B0302/B0303/B0304/B0305 anti-mouse Hashtag 1/2/3/4/5, respectively, Biolegend). Cells were washed, counted and concentrated to 1,000 cells / µl prior to pooling. Up to 25,000 cells (hyper-loading) pooled from 4 animals were loaded into a single well of a Chromium chip G (10x Genomics). 3' gene expression libraries were generated using Chromium Next GEM Single Cell 3' Kit 3.1 with 3' Feature Barcode Kit and dual indexing (10x Genomics protocol CG000316 Rev C or D). Libraries were sequenced as PE150 by Illumina sequencing to 65-80% saturation by the external provider Novogene.