GSM7025944: human dissection 3 intima; Homo sapiens; RNA-Seq
Each sample was washed, cut into small pieces, and digested in enzymatic digestion buffer with 3 mg/ml collagenase type II (LS004176, Worthington), 0.15 mg/ml collagenase type XI (H3506, Sigma), 0.25 mg/ml soybean trypsin inhibitor (LS003571, Worthington), 0.1875 mg/ml elastase lyophilized (LS002292, Worthington), 0.24 mg/ml hyaluronidase type I (H3506, Sigma), and 2.38 mg/ml 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, H4034, Sigma) in PBS with Ca2+ and Mg2+ at 37 °C for 60 minutes while shaking.