GSM7508040: ob2; Homo sapiens; RNA-Seq
Total RNA was extracted using the mirVana miRNA Isolation Kit (Ambion) following the  manufacturer's protocol. RNA integrity was evaluated using the Agilent 2100 Bioanalyzer (Agilent  Technologies, Santa Clara, CA, USA). The samples with RNA Integrity Number (RIN) ≥ 7 were  subjected to the subsequent analysis. The libraries were constructed using TruSeq Stranded Total