GSM7911535: MA9_FLT3ITD_Setd1b_WT_IL3_H3K4me3; Mus musculus; ChIP-Seq
Cells were fixed with 2mM DSG and 1% formaldehyde, and samples from sonicated nuclear fraction were incubated with each antibody and histone-DNA complexes were isolated. For histone modifications, DSG treatment was skipped. ChIP-seq libraries were prepared using NEBNext ChIP-Seq Library Preparation Set for Illumina (New England Biolabs) following the manufacturer's protocol.