GSM7061089: wild-type_rapamycin_rep2; Saccharomyces cerevisiae SK1; RNA-Seq
Cells were collected by centrifugation and then flash frozen in liquid nitrogen. RNA was extracted by AcidPhenol:Chloroform:Isoamyl alchohol isolation. PolyA selection and library generation was performed on 10ug of total RNA using NEXTflexTM Rapid Directional mRNA-Seq Kit