GSM7910387: 34A; Homo sapiens; RNA-Seq
Total RNA was isolated from ~30-50 mg white matter tissue using RNeasy Lipid Tissue Mini Kit (Qiagen, #74804) according to the manufacturer's instructions Globin mRNA was removed from total RNA using GLOBINclear (Invitrogen/Ambion). Next, RNA samples were subject to Ribo-zero depletion, and libraries prepared using the Illumina Truseq library preparation kit and sequenced on the Illumina HiSeq 2500 (2 x 50), generating a mean of 48 million reads per sample.