GSM8286453: CON_3; THP-1, Ctrl, 3h; Homo sapiens; RNA-Seq
Total RNA extraction was performed using Trizol LS reagent (Invitrogen) followed by purification using the AllPrep DNA/RNA kit, including the on-column DNAse treatment (Qiagen). The Stranded mRNA Prep kit (Illumina) was used to generate sequencing libraries according to the manufacture's reference guide #1000000124518, v02. Briefly, 10 ng (MDMs_ATP+CLM_6hr) or 25 ng (all others) total RNA was used as template for library preparation.