GSM6704003: 1st and 2nd trimester male and female gonads and mesonephros B; Homo sapiens; RNA-Seq
Tissue was cut in small pieces and treated overnight in 1mg/ml colII in 0.25% Trypsin/EDTA at 4C. Next day, it was incubated 1h at 37C in DMEM/F12 with DNAseI (27 IU/ml), filtered through a 100um strainer and single cells were cryopreserved. Before library preparation, cells were thawed, ressuspended in 1% BSA/DPBS and FACS-sorted for live cells (7AAD-negative cells). Sequencing libraries were prepared according to the user guide of the 10x Genomics Chromium Single Cell 3' Library and Gel Bead Kit v2, Product Code 120237.