GSM7849935: cka1, basal, rep2; Cryptococcus neoformans; RNA-Seq
The cells were then harvested by centrifugation and lyophilized. Total RNAs were isolated by Easy-BLUE (iNtRON), treated with DNase I (Qiagen), and puriﬁed with RNeasy MiniElute clean up kit (Qiagen) following the manufacturer's instruction Cultured samples were prepared independently three times for each strain, the cDNA library was constructed with the 1 µg of total RNAs for each sample by Illumina TruSeq mRNA library kit (Illumina) and sequenced by Illumina platform.