GSM8193453: PtrNAC127; Populus tremula x Populus alba; ChIP-Seq
leaf tissue was harvested and immediately cross-linked with 1 % formaldehyde under vacuum for 15 minutes at 25 °C. The cross-linking was stopped by adding glycine to the final concentration of 0.125 M for 5 minutes under vacuum. The cross-linked samples were washed three times with double distilled water. The ChIP assay based on GFP Monoclonal Antibody (Invitrogen, USA) was performed as described previously (Saleh et al., 2008; Li et al., 2014). Chromatin samples without GFP antibody immunoprecipitation were used as the control. The library was prepared by NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, USA).