GSM7881473: SUVKO 1928z-antibody capture Day 0 Rep-4; Homo sapiens; OTHER
Pre-infusion T cells (day 0), and flow-sorted CAR T cells from mouse bone marrow (day 9 and 16) were stained for 30 minutes at room temperature with a custom panel of 101 Total-Seq-C antibodies (Biolegend), and washed 3x using the HT1000 laminar wash system (Curiox). Cells were then counted using the Cellaca MX High-throughput Automated Cell Counter as described in the manufacturer's protocol (Nexcelom) and loaded on the 10x Chromium Next GEM Chip K Kit. TCR CDR3 sequences were enriched using the human V(D)J T cell enrichment. Libraries were prepared according to manufacturer's protocol (5' V2 kit, 10x Genomics) and sequenced on a NovaSeq 6000 System using the S4 2x 150 kit (Illumina).