GSM7754649: WT_D1_CYC-YPMF, rep1; Arabidopsis thaliana; ncRNA-Seq
Total RNA from flower tissue was extracted with TRIzol reagent, treated with DNaseI, and then purified again via phenol-chloroform extraction and ethanol precipitation. Fasteris NGS services (ngs@fasteris.com) of Genesupport SA constructed the small RNA-seq libraries using QIAseq-miRNA low input reagents. In brief, low molecular weight RNA was enriched using size-selective beads, 3' and 5'-adapters were successively ligated to the single-stranded RNA inserts, and cDNA synthesis was performed using reverse transcriptase. Each library was PCR amplified with adapters including sample-specific barcodes and a unique molecular identifier (UMI) region that can be used downstream for the bioinformatic elimination of PCR amplification bias. This service at Fasteris is known as the "Small RNA-Seq gel free protocol".