GSM8012576: Pupal abdominal epidermis, ATAC-seq, rep2; Drosophila melanogaster; ATAC-seq
For each replicate (n=3) we removed abdominal cuticles from 50 pupae 38-45 hAPF. We carefully removed internal organs with forceps in cold PBS, to retain only epidermal cells, which are attached to the cuticle. We followed the Omni-ATAC protocol (Corces et al. 2017) but using a lysis buffer based on IGEPAL detergent (Lysis Buffer: 10 mM Tris-HCl, pH=7.5, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630). Library concentration was quantified with a KAPA Library Quantification Kit (Roche) and quality control was performed with the High-Sensitivity DNA Analysis kit in a Bioanalyzer 2100 (Agilent).