GSM7911446: WT_U3; Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S; RNA-Seq
Total RNA was isolated using the High Pure RNA Isolation Kit (Roche) according to the manufacturer's protocol. Starting with 1 μg total RNA, ribosomal RNA was removed from the total RNA preparation using the Polaris Depletion Kit with custom rRNA depletion probes designed to target S. Typhimurium 14028s rRNA (Watchmaker Genomics). Libraries were prepared for Illumina sequencing by following the protocol accompanying the Watchmaker RNA Library Prep Kit. Samples were indexed and barcoded using full-length xGEN™ UDI-UMI Adapters (IDT, Index 1-4 and Index 9-12).