GSM7061313: F1 ram Sperm, CON, rep1; Ovis aries; ncRNA-Seq
the sperm were treated with somatic cell lysis buffer (0.1 % SDS, 0.5 % Triton X in DEPC H2O) for 40 min on ice to eliminate somatic cell contamination, followed by centrifugation at 600g for 5 minutes. The sperm pellet was washed and used for RNA extraction using TRIzol reagent. Small RNA libraries were constructed according to the NEBNext Small RNA Library Prep Kit protocol, employing 15 to 50 nt small RNA pre-size selection, followed by the standard cDNA library construction using NEBNext Small RNA Library Prep Kit and sequencing with Illumina NovaSeq 6000 platform.