GSM8149313: AR2lane2; Homo sapiens; RNA-Seq
Single cell suspension of surgically collected inferior turbinates were obtained by enzymatic digestion using liberase (87.5 µg/ml, 5401127001, Roche) and DNase I (300 µg/ml, 10104159001, Roche) in RPMI with 10% FBS for 30' with additional mechanical disruption using a 16G needle after both 15' and 30' of enzymatic digestion. Digestion was stopped by adding EDTA (20 mM, 15575020, Invitrogen), whereafter red blood cells were lysed using 9.9% NaCl solution. Negative selection for immune, myeloid and fibroblast was performed using anti-CD45 (11153D, Invitrogen), anti-CD15 (11137D, Invitrogen) and anti-CD31 (11155D, Invitrogen) dynabeads, respectively. Cells were resuspended in PBS and filtered through a 70 μm cell strainer (542070, Greiner). Library construction was performed according to manufacture's guidelines (singe cell 3' v2 protocol, 10X genomics)