GSM7429786: Left kidney, ctrl 1; Mus musculus; OTHER
24 hours after ischemia, mice were anesthetized. Immediately after dissection and de-capsulation, kidneys were snap-frozen in liquid nitrogen and embedded in optimal cutting temperature (OCT) at -80°C. 10 µm thick sections were cut on a cryostat at -15°C. Spatial transcriptomics was performed using 10X Visium Spatial Gene Expression Slide and Reagent Kit according to manufacturer's instructions, with the following sample-specific parameters: tissue sections were permeabilized for 12 minutes at 37°C. cDNA was amplified using 14 cycles based on Cq values obtained using qPCR. Based on post-amplification cDNA yield, final libraries were amplified using 13 cycles during sample index PCR. Each sample was indexed with a unique well from the Dual Index Kit TT Set A. Libraries were sequenced on an Illumina NovaSeq with an SP200 flowcell, 4 samples per lane, in paired-end mode.