GSM7881677: ATAC_seq_BCAS_hippocampus_4; Mus musculus; ATAC-seq
Nuclei was extracted from isolated hippocampus in 1 ml of lysis buffer (0.32M sucrose, 5 mM CaCl2, MgAce2, EDTA, Tris-HCl pH=8, w/o 0.1% NP-40), followed by washing in a sucrose buffer, and collected by centrifugation. ATAC-seq library was generated using reagents from TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme, TD501) according to the manuscript's instruction with modifications.