Short-read whole-genome sequencing of Psychrobacillus sp. L3
Total DNA was extracted from an overnight culture obtained from a single colony of strain L3 using Proteinase K/SDS treatment and Genomic DNA Clean & Concentrator-10 Kit (Zymo Research). The DNA was fragmented using a Covaris S220 focused ultrasonicator (Covaris) for a target fragment length of 550 bp. Fragmented DNA was then used as input to prepare Illumina MiSeq-compatible DNA libraries for 250 bp paired-end read sequencing using the TruSeq DNA Nano Low-Throughput Library Prep Kit as per the manufacturer's reference guide (using adapter #11 from TruSeq DNA Single Indexes Set B). The resultant library was sequenced on the Illumina MiSeq system as one of the several individually barcoded pooled libraries using 500-cycle MiSeq Reagent Kit v2 nano. PhiX sequencing control was used as a spike-in.