amplicon-based sequencing of SARS-CoV-2 in wastewater
Wastewater sequencing was performed on the BGI sequencing platform. Reverse transcription and amplification were performed using the ATOPlex RNA Multiplex PCR Amplification Kit V3.1 before library construction. DNA libraries were prepared using the MGIEasy Fast PCR-FREE digestion Library Preparation kit. Sequencing was completed on the DNBSEQ-G99 sequencing platform using the single-end sequencing 100 bp+ double-end barcode (SE100+10+10) strategy.