GSM7789852: C_2; Mus musculus; RNA-Seq
RNA was isolated from mice midbrain using Maxwell RSC simplyRNA Tissue Kit (Promega). The integrity of the RNA was subjected to quantity and quality control using Qubit HS RNA Assay Kit (Thermo Fisher Scientific) and 4200 Tapestation with High Sensitivity RNA ScreenTape (Agilent Technologies). All RNA samples were high-quality, with RIN values higher than 7. Library preparation was performed using the Illumina Stranded mRNA Prep Ligation kit (Illumina) following the manufacturer's protocol. All sequencing libraries were constructed from 100 ng of total RNA according to the manufacturer's instruction.