GSM8194975: JURKAT, oxamate and lactate, H3K18la; Homo sapiens; ChIP-Seq
Cell pellets were resuspended in 50μl nuclear isolation buffer (Sigma NUC-10) with added protease inhibitors. Chromatin digestion was performed using MNase (4-5U, NEB M0247) for 9-20 minutes Library construction was performed using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina following the manufacturer's protocol.