Q21.26.009.11.07.01
cDNA libraries were produced using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara 634894, Lots 1608207A and 1709695A) according to the manufacturers instructions, using 20 PCR cycles for cDNA amplification. Samples proceeded through library construction using Nextera XT DNA Library Preparation Kit (Illumina FC-131-1096) according to the manufacturers instructions except at either 0.5x, 0.4x, or 0.2x reaction size. Samples were sequenced to approximately 500 million paired-end 50b reads/sample