GSM6705530: TalKO-7122; Mus musculus; RNA-Seq
RNA was extracted from livers using the Qiagen RNeasy kit (Qiagen). Sequencing libraries were quantified using the KAPA Library Quantification Complete Kit Universal (KAPA Biosystems). The pooled library (1.4pM) was loaded onto the NextSeq 500 instrument, using the NextSeq 500/550 High Output v2 Kit for 75 cycles (Illumina). Cufflinks software was used to quantify gene expression and to detect transcript isoforms. Normalized data appear as Reads per Kilobase per Million reads (RPKM). Sequencing library preparation was done with the Illumina TruSeq Stranded Total RNA with RiboZero Gold kit following manufacturer's instructions.