GSM6603253: Sample_S41; Mus musculus; RNA-Seq
RNA was extracted from liver tissue from individual mice in four groups (OC or veh diet, with or without VWR, n = 5 per group) using the RNeasy Mini Kit per manufacturer's instructions including on-column DNase digestion. Total RNA was quantified using a Qubit RNA BR Assay Kit and RNA integrity was assessed on an RNA ScreenTape. mRNA-seq libraries (n = 5 per group) were prepared using a TruSeq Stranded RNA HT Sample Prep Kit (Illumina, Cat. No. RS-122-2303) on a Perkin Elmer Sciclone G3 platform. An additional standard 1.0x manual bead cleanup was performed using AMPure XP Beads (Beckman Coulter, A63881) after the libraries were completed to remove primer-dimers. Qubit dsDNA BR Assay kit was used to determine the concentration of the completed library and a Fragment Analyzer Standard Sensitivity NGS Fragment Kit Paired-end, opposing strand directional libraries