GSM8195218: 144b90ad-4280-44f8-8a5d-c973a064c406; Homo sapiens; ATAC-seq
RNA-seq: We extracted total RNA from lysed pellets frozen in RLT Plus buffer (collected on day 29) using the Quick-RNA™ MiniPrep Kit (Zymo Research), assessed quality to make sure RNA Integrity number (RIN) was 7.5 or greater. ATAC-seq: ATAC-seq samples were processed as previously described in detail25. Briefly, frozen nuclear pellets of 1x105 CVPC were thawed on ice and tagmented in total volume of 25μl in permeabilization buffer containing digitonin (10mM Tris-HCl pH 7.5, 10mM NaCl, 3mM MgCl2, 0.01% digitonin) and 2.5μl of Tn5 from Nextera DNA Library Preparation Kit (Illumina) for 45-75min at 37°