Plasmid sequencing of miniprepped, restriction digest linearized (SceI) : synthetic plsamid pool of barcodes and ORFs
Plasmids were miniprepped from E. coli using Qiagen miniprep kit, digested using I-SceI, observed to be linear and intact, then prepared for sequencing using PacBio ligation kit. Run on a Sequel 2 by UofArizona Genomics Core. Samples were apparenty not nick-repaired or treated with due respect, and reads appear truncated.