RNA-Seq of Nidirana pleuraden: adult male dorsal skin
Total RNA was isolated using the TRIzol reagent (Invitrogen, Carlsbad, CA, USA) followed by treatment with RNase-free DNase I (Promega, Madison, WI, USA) according to the manufacturers protocols. The concentration of extracted nucleic acid was detected with Nanodrop2000, (Thermo), and the integrity was examined with Agient2100, LabChip GX (Perkin Elmer). Illumina RNA-seq libraries were prepared for 48 samples and sequenced on Illumina NovaSeq 6000 platform with a PE150 strategy following the manufacturers instructions.