GSM7734216: NA04_48h, rep1; Rhodotorula toruloides; RNA-Seq
The WT, NA04, and NA14 were cultivated in the shake flasks containing 50 mL MMBOS medium and were sampled at 48h and 120h for transcriptome analysis. 1 mL diluted cell cultures with OD600 10 were centrifuged (6000 g at 4 ℃ for 5 min). The total RNA of the cell pellet was extracted using RNAprep pure kit (Tiangen, China) and assessed integrity using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). RNA libraries for RNA-seq were prepared using NEBNext Ultra RNA Library Prep Kit for Illumina®。