GSM7680851: ATAC_TF_Leu; Homo sapiens; ATAC-seq
Each sample containes 100,000 cells and the cell pellet was collected using moderately cold lysis buffer. The samples were centrifuged with 500 g, 4°C for 5 min. The cell pellet was resuspended with moderately cold lysis buffer, and library construction used the TruePrep DNA Library Prep Kit V2 for Illumina (TD502) (Vazyme Biotech Co., Ltd). DNA libraries were produced after 12 cycles of PCR amplification using the TruePrep library prep kit. After purification, the paired-end sequencing was performed on the Illumina Novaseq 6000 platform.