ncRNA-Seq of Mus musculus: 8-week-old female C57BL/6j eWAT
Total RNA of each sample was sequentially ligated to 3 and 5 small RNA adapters. cDNA was then synthesized and amplified using Illuminas proprietary RT primers and amplification primers. Subsequently, ~134-160 bp PCR amplified fragments were extracted and purified from the PAGE gel. And finally, the completed libraries were quantified by Agilent 2100 Bioanalyzer.