GSM8111967: SH, scRNAseq; Mus musculus; RNA-Seq
Take a small amount of single cell suspension, add an equal volume of 0.4% trypan blue dye, count the cells with Countess@IlAutomated cellCounter, and adjust the viable cell concentration to the ideal concentration (1000~2000 cells/uL) R1 (read 1 primer sequence) were added to the molecules during GEM incubation. P5, P7, a sample index, and R2 (read 2 primer sequence) were added during library construction via End Repair, A-tailing, Adaptor Ligation, and PCR. The final libraries contained the P5 and P7 primers used in Illumina bridge amplification.