GSM7029287: TL5_Hela_poly; Homo sapiens; OTHER
After labeling, cells were quickly washed in ice cold PBS before being scraped in mt-polysome lysis buffer. Mitoribosomes from monosome and polysome fractions were isolated after sucrose gradient fractionation. Extracted RNA was treated by TimeLapse-chemistry (0.1M sodium acetate pH 5.2, 4mM EDTA, 5.2% 2,2,2-trifluoroethylamine, and 10mM sodium periodate at 45°C for 1 hour). SMARTer Stranded Total RNA HI Mammalian kit (Takara)