GSM7597463: Mycelium, 0.25mg/mL Cyclo (Pro-Tyr), rep3; Botrytis cinerea; RNA-Seq
RNA from B. cinerea B05.10 was extracted from clumps after 24 h growth on PDB supplemented with 0.25 mg mL-1 of Cyclo (Pro-Tyr), and DMSO as control, at 28ºC 150rpm, using NucleoSpin RNA Plant and Fungi kit (Macherey-Nagel), according to the manufacturer's instructions. For RNA sequencing analysis, 200-500 bp single-end read libraries were prepared using a TruSeq stranded total RNA kit (Illumina). The libraries were sequenced using a NextSeq550 instrument (Illumina).