GSM6603237: Day 14, EBV-LCL cultured gamma-delta-T-cells, Donor 3 [FB]; Homo sapiens; RNA-Seq
The purified cells were stained with a panel of TotalSeq-C antibodies (Supp. Table M3) according to manufacturer's protocol and processed according to 10X Genomics' standard workflow for Chromium Single Cell V(D)J with Feature Barcoding technology for Cell Surface Protein to generate the transcriptome (5'GEX) and featured barcode library. To generate the TCRγδ V(D)J library, a 2-steps target enrichment PCRs were adopted from previously published protocol.