GSM7911809: Cebp_KO; Mus musculus; RNA-Seq
The fresh livers were collected after performing the insitu perfusion-digestion. The dissociated cells were purified based on 35% percol centrifugation. The cellular samples were loaded in the lanes according to the 10X Genomics 3' v3.1 Single Cell User Guide with a single capture lane per sample targeting recovery of up to 6,000 cells per lane. Cell partitioning completed successfully with uniform emulsion consistency for all samples. Reverse transcription PCR was run overnight. All subsequent steps GEM recovery, cDNA generation, and quality control were performed as described in the 10X Genomics 3' v3.1 Single Cell User Guide. Libraries were prepared using the 10X validated automation platform, Chromium Connect.