Fresh leaf of Physostegia virginiana (L.) Benth chloroplasts
After the sample genome DNA is tested to be qualified, break the DNA with enzymes, and then purify the broken DNA to build a sequencing library. The steps are as follows: repair the DNA end, add A to the 3 'end, break the DNA, and then purify the broken DNA to build a sequencing library. The steps are as follows: repair the DNA end, add A to the 3' end, connect the sequencing connector, recover the target segment by agarose gel electrophoresis, and PCR amplification of the target segment, Finally, a sequencing library was built. Before sequencing, perform quality inspection on the built library, and after passing the inspection, use Illumina NovaSeq6000 platform for sequencing