GSM7816160: Sinus+Artery, Klf4WT, YFPminus_control_16; Mus musculus; RNA-Seq
Single cell suspension was prepared and fluorescence activated cell sorting (FACS) was performed to separate YFP+ and YFP- cells. Each sample (condition) consisted of pooled aortic root/aortic arch/BCA/carotid arteries from 3 animals. YFP+ and YFP- samples for each condition were subjected to scRNA-seq using the Chromium Single Cell 3' Library and Gel Bead Kit (v3.1 10x Genomics, Pleasanton, CA) and the Chromium X.