GSM7850554: Efg1 WT biol rep 3; Candida albicans; RNA-Seq
RNA was purified from cells with the MasterPure Yeast RNA Purification Kit (LGC Biosearch Technologies) with DNAse I treatment. PolyA RNA was isolated with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) and use to create multiplexed libraries with NEBNext Multiplex Oligos for Illumina (Index Primers Set 1) (NEB).