16S rRNA of fermented seaweed
Culture-independent analysis of the microbiota was performed on DNA extracts prepared from the fermenting seaweeds. Briefly on days 2 and 7, microbial cells were harvested by centrifugation (10 min at 2,370g at 4 C) of volumes of 10 mL from the stomacher bags (101 dilution). DNA was subsequently extracted from the resulting pellet using the DNeasy PowerSoil Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions.