GSM7597451: H3K27ac NC_IP_2; Homo sapiens; ChIP-Seq
ChIP assay was conducted using SimpleChIP Enzymatic Chromatin IP kit (9002S, Cell Signaling Technology) according to the manufacturer's instruction. Chromatin was digested with micrococcal nuclease, and nuclear membrane was broken through several pulses. DNA fragment length was checked to be between 150–900 bp. Chromatin was immunoprecipitated by either control IgG or H3K4me3 (ab8895; Abcam; 2 μg) primary antibody and reverse cross-linked. The eluted DNAs were proceeded by QIAseq ultralow input library kit (QIAGEN, 180495) and quantified by qPCR.