GSM6602157: T9; Homo sapiens; miRNA-Seq
Total RNA including microRNAs (miRNAs) was isolated from the glioblastoma tissues and normal controls using the miRNeasy Serum/Plasma kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol. Total RNA was eluted in a volume of 30 μL RNase-free water. Concentration, purity and integrity for the RNA were de-termined by spectrophotometry (Little Lunatic, Unchained labs, CA, USA) and Agilent 2100 Bioanalyzer/Agilent RNA 6000 Nano Kit (Agilent, CA, USA). Library preparation including size fractionation with adapter ligation to 5' phosphate was performed by GENEWIZ Inc (GENEWIZ, NJ, USA).