whole genome sequencing of pristis pectinata: adult male
DNA was extracted from blood samples (50-100 microliters using Gentra Puregene (Qiagen) Blood kit in combination with MegaLong fragment separation kit using manufacturer protocols. DNA was suspended in 50 microliter elution buffer and quantified on a Qubit (ThermoFisher). Nanodrop (ThermoFisher) was used to calculate 260/280 and 260/230 ratios. Eight DNA extracts were sent to the University of California Davis DNA Technologies Core and processed by pulse-field electrophoresis before one sample was chosen for 10x sequencing. A library was prepared using the 10x Genomics Chromium Genome Reagent Kit (v2 Chemistry) and 10x Chromium system.