GSM7029480: cardiac mitochondria, control, 1; Mus musculus; miRNA-Seq
Total RNA was extracted from cells or frozen tissues using RNAiso Plus reagent (Takara Bio, Japan) according to the manufacturer's instructions. 1 ug of total RNA was used for the construction of sequencing libraries. Total RNA was ligated with the 3' end junction and the 5' end junction, respectively. First strand of cDNA synthesis was performed using random primer, followed by library enrichment (11-12 cycles of PCR) and library purification (6% Novex TBE PAGE gel, 1.0 mm, 10 well). Quantification was performed with TBS380 (Picogreen). Then samples were mixed on the machine in proportion to the quantitative data, followed by bridge PCR amplification on cBot to generate clusters.