Control_BV2
BV2 cells treated with or without glucose at the concentration of 100 mM for 72 hours were used for circRNA sequencing. The RNA of BV2 cells were extracted and purified, followed by rRNA removal and linear RNA digestion. The remaining circRNA were fragmented and cDNA synthesis were performed to construct the library. Single-stranded PCR products are produced via denaturation. After digesting uncyclized linear DNA molecules, single-stranded cyclized products are produced for sequencing.