GSM7029370: wt3; Mus musculus; RNA-Seq
RNA was extracted using RNeasy Firbous Tissue kit (Qiagen). 1 µg of total RNA was used for the construction of sequencing libraries. Directional libraries were prepared from total RNA using the Truseq Stranded mRNA sample preparation kit (Illumina 15031047) following the manufacturer's instructions. Clusters were generated for the resulting libraries, with Illumina HiSeq SR Cluster Kit v3 reagents. Runs were carried out over 51 cycles, including seven indexing cycles, to obtain 51-bp single-end reads.