GSM7062306: HOCL + E07 10; Mus musculus; RNA-Seq
Total RNA was extracted from whole blood collected in RNA protect tubes (Qiagen) and were proceeded for extraction using the RNeasy Protect Animal blood kit with the optional on-column DNAse I digestion At least 250 ng of purified RNA samples were used as input for the TruSeq Strand-specific RNA-seq (poly A selection and globin depletion) according to the manufacturer's instructions (Illumina) Following the preparation of libraries, samples matching the requested criteria were sequenced on a NovaSeq 6000 sequencer (Illumina).