GSM8150775: Pooled Gene Expression of hiPSC-CFs; Homo sapiens; RNA-Seq
hiPSC-CFs were dissociated with TrypLE, centrifuged for 5 minutes at 300g, and the pellet was snap-frozen for snRNA-seq Cell pellets were barcoded with 3' CellPlex Kit from 10X Genomics. After barcoding the cells, nuclei were extracted, washed, and pooled. Library was constructed according to the manufacturer's instruction for Chromium 3' v3.1 dual index kit.