GSM7912135: RP-2; Bos taurus; ncRNA-Seq
Total RNA from the NC and RP groups was isolated and purified using TRIzol reagentt (Thermo Fisher Scientific, Waltham, MA, USA). After the construction of the sequencing library is completed, Qubit is used for preliminary quantification and the library is diluted to 1ng/ul. Then, Agilent 2100 Bioanalyzer is used to detect the insert size of the sequencing library. Once the insert size meets expectations, qPCR method is used to accurately determine the effective concentration of the library (the effective concentration of the library is>2nM) to ensure the quality of the library.