snATAC-seq of mus musculus: adult male amygdala BL6
After microdissection of amygdala tissue, single nuclei suspension and library generation followed the 10X protocol for Next GEM scATAC-Seq v1.1 (PN-1000175) as described except for the following modifications: Nuclei suspensions were generated using the recommended method from the 10X scMultiome protocol (CG000375 Rev C) to lyse cells and obtain nuclei. Following single nuclei suspension generation, nuclei were counterstained for 7-AAD and sorted on a MACSQuant Tyto prior to GEM generation. After the sort, we carried out permeabilization of nuclei as per the protocol.We aimed to capture 10,000 nuclei per well x 8 wells, for a total of 80,000 nuclei over 8 total samples (~10,000 nuclei per genotype per region per replicate). All downstream library preparation was done according to the 10X protocol (CG000209) and sequenced on a Novaseq 6000 with a target of >35k reads per nucleus.