GSM7029246: TL8_Hela_50_0m_IP_repA; Homo sapiens; OTHER
After labeling, cells were quickly washed in ice cold PBS before being scraped in mt-polysome lysis buffer. Mitoribosomes were isolated after sucrose gradient fractionation and immunoprecipitated with an MRPL12 antibody. Extracted RNA was treated by TimeLapse-chemistry (0.1M sodium acetate pH 5.2, 4mM EDTA, 5.2% 2,2,2-trifluoroethylamine, and 10mM sodium periodate at 45°C for 1 hour). SMARTer RNA unique dual index kits (Takara)