GSM7988743: WT_S7_1; Homo sapiens; RNA-Seq
RNA was extracted from HEK293T cells using the PureLink RNA Mini Kit (Invitrogen) and converted to complementary DNA (cDNA) using the iScript cDNA Synthesis Kit (Bio-Rad). The RNAseq libraries were prepared with the Kapa Hyper Stranded mRNA library kit (Roche).  The libraries were pooled; quantitated by qPCR and sequenced on one S1 lane for 151 cycles from both ends of the fragments on a NovaSeq 6000 with V1.5 sequencing kits.