GSM8113507: Memory_SF_ATAC_rep1; Homo sapiens; ATAC-seq
50,000 HUVEC were harvested, resuspended in ice-cold DPBS, and centrifuged (1 min at 1300 x g, 4°C). Pelleted cells were resuspended in 50 μL of cold lysis buffer and centrifuged (10 min at 1200 x g, 4°C). After discarding supernatant, 50 μL of transposase mix with 2.5 uL of Tn5 transposase (Illumina) were added and incubated for 30 min at 37°C. The reaction was stopped by purification with MinElute PCR purification kit (Qiagen) following manufacturer's instructions. Library amplification by PCR was done after determining number of cycles needed. Products were size-selected with Agencourt AMPureXP beads (Beckman Coulter) and sequenced on a Nextseq 500 System (Illumina, 75 bp paired-end reads).