RNA-seq of Scorpio
In this project, the mRNA with polyA structure in total RNA was enriched by Oligo(dT) magnetic beads, and the RNA was interrupted to a fragment of about 300bp in length by ion breaking.RNA was used as template to synthesize the first strand of cDNA using a 6-base random primer and reverse transcriptase, and the first strand cDNA was used as template for the second strand cDNA synthesis. After library construction, PCR amplification was used for library fragment enrichment, after which library selection was based on fragment size, which was at 450bp.