DRAFT GENOME SEQUENCE OF Shigella sp. strain KRA01
Whole genome sequencing of bacterial strain KRA01 was done using Illumina noveseq 6000 platform and DNA library was prepared. The raw data quality was checked using FatQC and MultiQC software. The raw sequence reads were processed to remove adapter sequences and low-quality bases using fastp v0.12.4 with default parameters. The QC passed reads were assembled to contigs using Unicycler v0.5.0. Contigs shorter than 200 bp were removed from the assembly. The assembled genome statistics were assessed using QUAST v5.0.2. The assembly quality was checked by mapping the reads back onto the assembled contigs using bowtie2 v2.4.5. The genome completeness was checked using BUSCO V5.3.2 with bacteria_odb10 as reference. The genome completeness and contamination were also checked using CheckM v1.2.1 tool.