GSM7816380: SKMEL28 cells, shIRF4, day 3,  biological replicate 1; Homo sapiens; RNA-Seq
RNA was extracted using the Roche High Pure RNA Isolation kit. 1.1 mg total RNA  was used for generating RNA-seq libraries TruSeq RNA Sample Preparation Kit was used for library preparation step. Briefly, library preparation starts with purification of mRNA by poly-A selection using oligo-dT attached magnetic beads. Following purification, mRNAs are fragmented and copied into first strand cDNA by using random hexamers. Then second strand cDNA synthesis is performed. End repair and addition of adenine base follows the procedure. At the end, adaptors with barcodes are ligated and products are enriched with PCR.