V1V3 amplicon from oral mucosal swab
Amplification of the V1V3 region of the 16S rRNA gene was carried out using the primers 27F-YM and 519R (27F-YM: 5-AGAGTTTGATYMTGGCTCAG; 519R:  5-GWATTACCGCGGCKGCTG) and was performed in duplicate using separate template dilutions (1:1 and 1:10). Amplification and sequencing was performed by Integrated Microbiome Resources (IMR; Dalhousie, Canada) according to their standardised, in-house protocols detailed here: https://www.protocols.io/workspaces/integrated-microbiome-resource-imr/publications. Paired end sequencing was performed using the Illumina 600 cycle MiSeq reagent kit.