RNA-seq T.gondii
RNA samples were collected after infecting HFF cells with the RH strain for 24 hours and then treated for 6 hours with either DMSO or MC1742 (0.5 M). Parasites were extracted and purified from the host cell before being lysed in Trizol. RNA was extracted as per manufacturers instruction and genomic DNA was removed using the an RNase-free DNase I Amplification Grade Kit (Sigma). All RNA samples were assessed for quality using an Agilent 2100 Bioanalyzer. RNA samples with an integrity score  8 were included in the RNA library preparation. Triplicates (biological replicates) were produced for each condition. The TruSeq Stranded mRNA Sample Preparation kit (Illumina) was used to prepare the RNA libraries according to the manufacturers protocol. Library validation was carried out using DNA high-sensitivity chips passed on an Agilent 2100 Bioanalyzer. Library quantification was carried out by quantitative PCR.