GSM7733685: K562 cells, early S-Phase (S2); Homo sapiens; RNA-Seq
RNA was extracted with TRI Reagent® (MRC). RNA samples were treated with DNase I: 10 U of DNase I (Roche) per 3 mg of total RNA; 37ºC for 1 hr in the presence of RNase A inhibitor. Nascent RNA capture was performed using Click-iT® Nascent RNA Capture Kit (ThermoFisher) according to the manufacturer's instructions with minor modifications. Transcriptome library construction (with BGI Genomics)