MobiSeq of Aphanius iberus: Adult tail fin
One Aphanius iberus sample (Sample Name: Chi_6_lcWGS) was selected for low-coverage whole genome sequencing to serve as a reference for identifying transposable elements (TEs) throughout the genome of A. iberus. A candidate TE for the design of the TE-target primer was selected and library construction for all the samples was performed through PCR-targeted enrichment of the flanking regions of the selected candidate TE based on the MobiSeq protocol outlined by Rey-Iglesia et al., (2019). The resulting pool of samples was multiplexed and sequenced simultaneously on a single flow cell on an Illumina NovaSeq 6000 with a paired end 150 cycle protocol, aiming for a total output of 110 gigabases.