GSM8112497: Male, Donor 4,  Outgrowth; Homo sapiens; RNA-Seq
The constructs collected were immediately preserved in Trizol reagent (Life Technologies, United States) and stored at -80°C until RNA extraction. The PuroSPIN Total RNA Purification KIT (Luna Nanotech, Canada) was used for RNA extraction and purification according to the manufacturer's instructions. Raw input reads were trimmed and aligned to the reference human genome hg38 using the STAR 2.7.3a aligner. Genes with maximum read counts below 50 were filtered out, and normalization was performed using the Add 1.0, TMM, and Log 2.0 parameters. Statistical analysis was conducted using ANOVA with biological sex and time points as factors.