GSM6705461: fndc1_3; Mus musculus; RNA-Seq
Differentiated for 4 days in high glucose medium with 2% horse serum, samples were collected. Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) according to the manufacturer's procedure. Then 10 ug of total RNA was extracted according to the RNA-Seq sample preparation kit for constructing sequencing library. RNA libraries were prepared for sequencing using standard Illumina protocols