GSM7063747: 1.25do seeds, Curio slide-seq; Arabidopsis thaliana; OTHER
Germinating seeds (1.25d) were submerged in OCT in a cryomold (Sakura), and flash frozen in a liquid N2 cooled isopentane bath. Tissue blocks were acclimated to -18oC in a pre-cooled cryostat chamber (Leica) for 1 h. Tissue blocks were trimmed until the tissue was entered, after which 10 µm sections were visually inspected until the region of interest was exposed. Following sectioning, the frozen section was arranged onto the center of the Curio Seeker Tile (Curio Bioscience) and gently melted and stored at -80oC until further processing according to the manufacturer's specifications. Library construction was performed according to the manufacturer's specification (Seeker Tile Kit, Curio Bioscience)