GSM6602935: WT_1; Mus musculus; RNA-Seq
Dissected hippocampi were dissociated using the Adult Brain Dissociation Kit (Miltenyi Biotec #130-107-677) according to the manufacturer's instructions. Cell suspensions were applied to 70 mm pre-moistened cell strainers and cells were collected by centrifugation for 10 min at 300 xg, 4 oC. Cell pellets were resuspended in ice-cold D-PBS using wide-bore, pre-moistened tips, prior to debris removal, which was carried out according to the manufacturer's instructions. Final cell pellets were resuspended in 0.04% BSA in D-PBS (Miltenyi Biotec MACS BSA #130-091-376). Cell viability was assessed using a NanoEntek Eve Automated Cell Counter. The single-cell suspension was then loaded into the 10x Chromium. Library was performed according to the manufacter's instructions (single cell 3' v1 protocol, 10x Genomics).