Nano-DMS-MaP of HCV IRES: IVT 8mM DMS rep1
in vitro transcription of HCV IRES sequence (with 3' M13 rev adapter sequence), in vitro folding, DMS probing, RNA purification by sodium acetate-ethanol precipitation, reverse transcription with primer M13_pA_re, PCR with primers HCV_IRES_fw and M13_pA_re, and sequencing with native barcoding kit Nanopore SQK-NBD112.96