GSM7508811: Mandibular Process (Md), rep2; Mus musculus; ATAC-seq
Freshly dissected mouse embryonic face tissue. Approx. 50'000 nuclei were pelleted at 500 RCF for 10 min at 4°C and resuspended in 50 µL Transposition reaction mix containing 25 µL Nextera 2x TD buffer and 2.5 µL TDE1 (Nextera Tn5 Transposase; Illumina) (cat. no. FC-121-1030) followed by incubation for 30 minutes at 37°C with shaking. The reaction was purified using the Qiagen MinElute PCR purification kit and amplified using defined PCR primers ATAC-seq libraries were purified using the Qiagen MinElute PCR purification kit (ID: 28004), quantified by the Qubit Fluorometer with the dsDNA HS Assay Kit (Life Technologies) and quality assessed using the Agilent Bioanalyzer high sensitivity DNA analysis assay. Libraries were pooled and sequenced using single end 50 bp reads on a HiSeq 4000 (Illumina).