GSM7061600: IMR90_NMN_rep1(CUT&Tag); Homo sapiens; OTHER
CUT&Tag experiments were performed according to the CUT&Tag Assay Kit manual (Vazyme, TD903). Briefly, cell pellets were collected at 600 × g for 5 min, washed with wash buffer, and incubated with ConA beads for 10 min at room temperature in 8-strip tubes. Subsequently, the cell–ConA bead complexes were incubated with anti-H3K9la (PTM BIOLABS, PTM1419RM) at 4℃ overnight and a secondary antibody for 1 h at room temperature. The pA/G-Tnp transposon mediated the transposition reaction, and TTBL mediated DNA fragmentation. DNA extraction beads were used to extract CUT&Tag-enriched DNA CUT&Tag libraries were prepared for sequencing using established protocols