RNA-seq of Schmidtea nova: whole wild type worms
Total RNA extracted from snap-frozen planarian tissue using the protocol described in Liu, SY. and Rink, J.C. (2018). RNA was purified using a Clean & Concentrator-25 kit (Zymo). The poly-A+ fraction of RNA was isolated using Oligo d(T)25 Magnetic Beads (New England BioLabs Inc.). The resulting poly-A+ RNA fraction was then purified again on a Clean & Concentrator-5 Column Kit (Zymo). The cDNA libraries for Oxford Nanopore Sequencing were prepared using the PCS111 kits, starting from 100ng cDNA. Sequencing was performed on a FLO-MIN106D R9.4.1 flow cell.