Illumina HiSeq 1500 paired end sequencing; RNA-seq of dmGluRA[112b] mutant Drosophila against controls
RNA-seq of dmGluRA[112b] mutant Drosophila against controls
10 day old adult female flies were snap frozen in liquid nitrogen. Total RNA was isolated from whole adult flies by homogenisation in TRIzol  reagent (Invitrogen) according to the manufacturerâ€™s protocol. Following chloroform extraction, RNAs were purified using RNeasy Mini columns (Qiagen) including an on-column DNase digestion.  RNA-seq libraries were prepared using a TruSeq Stranded mRNA Library Prep kit (Illumina). Libraries were quantified on a Bioanalyzer (Agilent Technologies).