RNA-seq of Sus scrofa: ASFV-DeltaI8L infection 4 h
PAMs were infected with ASFV-DeltaI7L, ASFV-DeltaI8L, or ASFV-WT (ASFV HLJ/18) at an MOI of 1. At 4, 12, and 20 hpi, the cells were used to extract total RNAs by using TRIzol reagent (catalog no. 15596026; Invitrogen) according to the manufacturers instructions.The Genome-wide differential gene expression analyses were performed using high-throughput sequencing by BGI Genomics (Shenzhen, China).