Whole genome sequencing of HAC Cells: Clone 2
Genomic DNA was prepared from a 10-cm plate of cells using the Qiagen Dneasy Blood and Tissue Kit according to the standard protocol. 500ng was prepped with Illumina on-bead tagmentation. Indexed with IDT for Illumina DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples). Index used was D1. 5 PCR cycles were used for amplification. Each unenriched library was pooled at an equimolar amount and 24X that of each enriched library before loading on the S2 flow cell.