GSM8062430: Biology Group 3, sample 1; Sus scrofa; RNA-Seq
RNA was extracted using the Nucleospin RNA plus kit (Macherey Nagel) and following manufacturer's instruction. The RNA concentration was evaluated on NanoDrop (Thermo Fisher). The resulting RNA quality was determined using a Bioanalyzer Instrument (Agilent) and all samples had RNA integrity numbers above 8.1. RNA sequencing libraries were prepared according to Illumina's protocols using the Illumina Stranded mRNA Prep, Ligation kit (reference no. 20040534). Ten cycles of polymerase chain reaction (PCR) were applied to amplify the libraries. Library quality was assessed using a LabChip GXII Touch Instrument (PerkinElmer) and libraries were quantified by qPCR using the NEBNext Library Quant Kit (E7630), on the LightCycler 480 II Instrument (Roche).