DNA of Klebsiella isolated from environmental plastic pollution (polyethylene)
Ligation sequencing libraries were prepared using the NEBNext ONT Companion Module (New England Biolabs, UK) and the Native Barcoding Kit 24 (SQK-NBD112.24; Oxford Nanopore Technologies, UK), following the manufacturers protocols. The library was sequenced for 48h using an Oxford Nanopore MinION (Mk1C) flowcell (FLO-MIN106; Oxford Nanopore Technologies, UK).