GSM8113777: NIH3T3, H2A.Z, CFDP1 siRNA ChIP 2; Mus musculus; ChIP-Seq
Control and treated cells were fixed with 1.1% Formaldehyde for 10 min at room temperature and quenched with 125mM Glycine. Extracted nuclei were sonicated using Q700 Sonicator system (Qsonica). ChIP seq library was constructed using 5ng of ChIP DNA using KAPA HTP Library Preperation Kit (Kapa Biosystems). Library amplication was performed for 13 cycles in a thermocycler. Amplified libraries were size selected for 250-500bp range using AMPure XP beads (Beckman Coulter). Library size and distrubution was checked on Agilent Bioanalyzer.