DNA methylation sequencing of Alligator sinensis:Muscle of hindlimb
After the sample DNA test was qualified, a certain proportion of lambda DNA was added as Bisulfite conversion test, and Covaris M220 was used to randomly interrupt genomic DNA to 200-400bp. End repair of broken DNA fragments, adding A tail, and methylated sequencing splices; Then Bisulfite treatment (using EZ DNA Methylation Gold Kit, Zymo Research) was performed. After treatment, the unmethylated C became U (T after PCR amplification), while the methylated C remained unchanged, and finally PCR amplification was performed. Get the final WGBS library