GSM7988452: S05__BPCMML__PT_993T5__BM_blast; Homo sapiens; RNA-Seq
Total RNA was extracted from primary sorted cells using the RNeasy Plus Micro Kit (74034, Qiagen), as per manufacturer instruction. RNA quality was quantified via Qubit and analyzed using the Agilent Bioanalyzer. Libraries were generated using the NEB Next Ultra II (pA) RNA (E7770, New England Biolabs) kit following a 10ng input workflow. Libraries (1x101bp) were sequenced using the NovaSeq 6000 sequencing system (Illumina), installed with SP type flow cells, aiming for 45 million reads per sample.