GSM7850729: ce_smallRNA_longTC_N2_24h; Caenorhabditis elegans; ncRNA-Seq
We harvested worms from 5h to 48h in hourly interval. Worms were collected from plates and washed 3 times and resuspended in Tri Reagent (MRC, TR118) and RNA was extracted using conventional phenol extraction, as described previously (Hendriks et al., 2014). Total RNA was DNAse treated Sequencing libraries were generated using Illumina TruSeq Small RNA Library Prep Kit according to the manufacturer's instructions. Subsequently, library was sequenced on Illumina HiSeq2500, as 51 cycles single-end reads.