Clonal evolution during metastatic spread in high-rish neuroblastoma: Sample I-H-118706-T15-1-D1-1
Tumor DNA were extracted from fresh frozen or OCT tumor biopsies and mathced normal DNA from buffy coat, bone marrow or peripheral bone marrow cells using the DNeasy Blood and Tissue Kit (Qaigen catalog #69504). After PicoGreen quantification and quality control by Agilent BioAnalyzer, 500ng of genomic DNA were sheared using a LE220-plus Focused-ultrasonicator and WGS sequencing libraries were prepared PCR-free using the KAPA Hyper Prep Kit (Kapa Biosystems. KK8504). Samples were run on Illumina HiSeq2000, HiSeq 2500, HiSeq X or NovaSeq 6000 machines.