GSM8425656: Hepa1-6 cells  NC-3; Mus musculus; RNA-Seq
RNA library preparation and high through-put sequencing and data analysis were conducted by Seqhealth Technology Co., LTD (Wuhan, China). 2 μg total RNAs were used for stranded RNA sequencing library preparation using KCTM Stranded mRNA Library Prep Kit for Illumina® (Catalog NO. DR08402, Wuhan Seqhealth Co., Ltd. China) following the manufacturer's instruction. PCR products corresponding to 200-500 bps were enriched, quantified and finally sequenced on Novaseq 6000 sequencer (Illumina) with PE150 model. 1 μg total RNAs were used for stranded RNA sequencing library preparation using Ribo-off rRNA Depletion Kit (Human/Mouse/Rat) (Catalog NO. N406, Vazyme) and KC-DigitalTM Stranded mRNA Library Prep Kit® (Catalog NO. DR08502, Wuhan Seqhealth Co., Ltd. China) following the manufacturer's instruction.