GSM6801745: S14,COPD,typical rate of COPD progression; Homo sapiens; miRNA-Seq
A 2-3-mL peripheral blood sample was collected from each participant into the PAX-gene Blood RNA Tube. After gradient cooling, the samples were stored at –80°C. Total RNA was extracted from the blood samples using TRIzol reagent. The purity and concentration of extracted RNA were detected using Nanodrop (Optical Density (OD)260/280 ≥1.8; OD260/230 ≥ 1.0) and Qubit 2.0 (total RNA concentration ≥250 ng/µL), respectively. The integrity of the RNA sample was detected using Agilent 2100 bioanalyzer to ensure that samples qualified for sequencing (RNA integrity number of total RNA ≥8.0, 28S/18S ≥1.5; the baseline of the map was not raised; the 5S peak was normal). The high-throughput sequencing experiment of miRNA was performed by Beijing Baimaike Biotechnology Co., Ltd. through the technical service contract, which mainly included several key procedures of miRNA isolation and purification, library establishment, and sequencing.