GSM7431923: U2932 cells, miR-28,20h, rep1; Homo sapiens; RNA-Seq
RNA was harvested using miRneasy mini  kit (Qiagen). Total RNA was used to generate barcoded RNA-seq libraries using the NEBNext Ultra II Directional RNA Library preparation kit (New England Biolabs) according to manufacturer's instructions. First, poly A+ RNA was purified using poly-T oligo- attached magnetic beads followed by fragmentation and first and second cDNA strand synthesis. Next, cDNA ends were repaired and adenylated. The NEBNext adaptor was then ligated followed by second strand removal, uracile excision from the adaptor and PCR amplification Libraries were sequenced on a HiSeq4000 (Illumina) to generate 60 bases single reads