GSM8324697: NOK_RNA_SLT_2h_3_S27; Homo sapiens; RNA-Seq
After exposure, the media containing the test substances were removed from the flasks and the cellular RNA was extracted using standard methods (TRIzol™ Reagent, Cat. No. 15596026, Invitrogen). Estimation of yield was done by Qubit analysis (Qubit 3.0 Fluorometer, Life Technologies, Invitrogen™, Thermo Scientific Fisher) . RNA integrity was analysed on High Sensitivity RNA ScreenTape (Cat. No. 5067- 5579, Agilent), using the 4200 TapeStation automated electrophoresis instrument (Agilent). All samples exhibited RNA Integrity Number (RIN) above 8.4. 500 ng RNA per sample was used to prepare libraries (QuantSeq 3' mRNA-Seq Library Prep Kit for Illumina (FWD)), Cat. No. 015, Lexogen). QuantSeq 3' mRNA-Seq Library Prep Kit for Illumina (FWD)) (Lexogen)