GSM7061654: hTERT cells, Spheroid-MED12-G44 mutant colony-1, rep-1; Homo sapiens; RNA-Seq
Total RNA was isolated using the Zymo research Quick RNA miniprep kit utilizing the on-column DNAse treatment according to the manufacturer's instructions (#R1054).500 ng of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEBNext #E7490) and NEBNext® Ultra™ Directional RNA Library Prep Kit (NEBNext #E7420)  following manufacturer's protocols.