GSM7851881: Myco Feriruminatoris 1_b; Mycoplasma feriruminatoris; RNA-Seq
Cells separated from supernatant by centrifugation at 10,000 rpm and washed 3 times in PBS 1x. Bacterial pellet treated with RLT buffer (Rneasy kit, Qiagen) supplemented with 1:100 beta-mercaptoethanol. The rest of the protocol was followed as per the manufacturer's instructions. RNA libraries were prepared for sequencing using standard Illumina protocols polyA-selected stranded RNA-seq