PacBio sequence reads of the Emydomyces testavorans genome
E. testavorans strain 16-2883 was grown in SD medium with keratin. Spheroplasts were created by zymolyase treatment then lysed with SDS. The sample was phenol/chloroform extracted and precipitated with isopropanol. Following RNAse and proteinase K treatment, DNA was sheared with a Megaruptor3 to an average fragment length of 10 kb. The library was constructed with the SMRTBell Express Template Prep kit 2.0 (Pacific Biosciences) and sequenced on one SMRTcell 8M using a PacBio Sequel IIe with the CCS sequencing model and a 30 h movie time. CCS and demultiplexing analysis was done using SMRTLink V11.0.