GSM7989090: LNCaP_RNAseq_M0_rep3; Homo sapiens; RNA-Seq
The total RNA was extracted from LNCaP cells by following instructions from RNeasy Plus Mini Kit (Qiagen, Valencia, CA). Library was constructed at Novogene. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.