Metagenome of marine bacteria from South Pacific
1 g of DNA was used as input material. Sequencing libraries were generated using the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, USA). 350 bp DNA fragments (obtained by sonication), were barcoded, end-polished, A-tailed, and ligated with the full-length adapter for Illumina sequencing with further PCR amplification. PCR products were purified using the AMPure XP system, libraries were analyzed for size distribution by Agilent2100 Bioanalyzer and quantified using real-time PCR, and sequenced on an Illumina Novaseq6000 with paired-end 150 bp configuration.