ChIP-seq of Schmidtea mediterranea CIW4: wild type, Input for H3K4me3
Library preparation was performed with purified ChIP-DNA using the NEBnext Ultra II DNA library preparation chemistry (New England Biolabs, E7370L). DNA fragments were end-repaired, A-tailed and ligated to unique-dual indexed Illumina Truseq adapters. Resulting libraries were PCR-amplified for 15 cycles using universal primers, purified using XP beads (Beckman Coulter) with a bead to library ratio of 1:1. Size selection was done using XP beads with a 0.6:1 and 1:1 bead to library ratio and if needed subjected to an extra 0.8:1 bead to library ratio to remove the left-over adaptor dimers.