RNA-seq of Apis mellifera scutellata: day 10 Mandibular gland
Total RNA was isolated using TRIZol and treated with DNase. RNA quality and quantity was measured using a Nanodrop 2000 and samples sent for sequencing at BGI in Hong Kong. All samples had QC Class A classification with RIN on >7.4. cDNA libraries were constructed using the Illumina TRISeq RNA Preparation kit generating 100 bp paired ended fragments.