ONT WGS of Elysia crispata: brain
Genomic DNA was extracted from E. crispata brains dissected from wild adults. A CTAB-based protocol was used to generate high molecular weight DNA. Short DNA fragments less than 10 kbp were depleted using a SRE XS kit (Circulomics, Baltimore, MD) according to the manufacturers protocol. At least 1.9 g of high molecular weight genomic DNA was used as input for ONT LSK-109 library ligation kits and sequenced on an R9 MinION flow cell. Base calling of ONT reads was performed with Guppy v6.1.2 (Oxford Nanopore Technologies, Oxford, UK).