Butyrate prevents visceral adipose tissue dysfunction and metabolic alterations in a mouse model of Friedreich's ataxia
Butyrate prevents visceral adipose tissue dysfunction and metabolic alterations in a mouse model of Friedreich's ataxia
Upon collection, fecal pellets were immediately preserved by freezing in liquid nitrogen and then stored at -80°C.  To extract fecal nucleic acid, an E.Z.N.A. stool DNA kit (OMEGA, Bio-tek) was used. The bacterial 16S rRNA gene was amplified from total DNA following the Illumina 16S Metagenomic Sequencing Library Preparation instructions, targeting the V3-V4 hypervariable region amplicon by PCR with universal primers containing Illumina adapters