GSM7732621: P3F + iPSC EC Diff Day 15 IgG C&R; Homo sapiens; OTHER
Nuclei were isolated by incubating cells in Nuclear Extraction Buffer (NEB) which consists of 20mM HEPES KOH pH7.9, 10mM KCl, 0.1% Triton X-100, 20% Glycerol, EDTA-free Protease Inhibitor (11836170001, Roche) and water for 10 minutes on ice. Aliquots of 250,000 nuclei were spun down then resuspended in nuclear extraction buffer and slowly frozen at -80°C in a Mr. Frosty freezing container (C1562, Sigma-Aldrich). Libraries were prepared with the xGen ssDNA & Low-Input DNA Library Prep Kit (#10009859, IDT) and quantified using the Agilent 4150 TapeStation and Agilent Bioanalyzer before sequencing with NovaSeq6000 (Illumina).