GSM7431301: WT, DMS, rep 2; Escherichia coli str. K-12 substr. MG1655; OTHER
Total RNA was extracted using the RNeasy MINI kit (Qiagen) and then ribosomal RNA was depleted using RiboMinus (Thermo Fisher) Structure-seq2 libraries were prepared by reverse transcription with a random hexamer primer, ligation of a hairpin adapter, and then amplification incorporating Illumina-specific primers.