RNA-Seq of Mouse liver 1, WT control
High-quality total RNA was isolated from liver tissues of female WT and PXR-KO mice (n=5/treatment/genotype) fed control or HFD for 52 weeks. RNA extraction utilized Trizol, followed by purification using RNeasy spin columns. RNA concentrations were quantified with the Qubit RNA HS assay, and quality assessed using Agilent Tapestation4200 RNA Screentape. Samples with RNA integrity number >7 were selected for library preparation. cDNA fragmentation was performed for 6 min at 85 C. Libraries were generated using the Kapa Stranded mRNA Library Kit and subsequently multiplexed and pooled. Paired-end sequencing (2x100 cycles) was conducted on an Illumina Novaseq 6000 platform. Data analysis encompassed genome alignment with STAR, gene expression analysis using DESeq2, and generation of quality reports with fastQC. This comprehensive pipeline was executed at the HTSF and BARC facilities for robust results