GSM7852425: Patient 2 biopsy B; Homo sapiens; RNA-Seq
RNA was purified using RNA STAT-60 (AMS Bio) according to manufacturer's instructions and treated using Amplification Grade DNase I (Invitrogen) followed by ethanol precipitation and clean-up. Quality control, library preparation and sequencing were performed by the Wellcome Trust Centre for Human Genetics. Libraries were prepared using the Illumina TruSeq Stranded mRNA sample prep kit according to manufacturer's instructions. Single-end 75bp sequencing was performed on Illumina HiSeq4000.