small RNA libraries from the control flag leaves of Replicate 2 of Oryza coarctata
Total RNA was isolated from tissues using Qiagen RNA MiNeasy kit (Qiagen Inc, USA) with DNase treatment. The Total RNA quality was verified using RNA 6000 Nano Kit (Agilent Technologies, USA) on 2100 Bioanalyzer (Agilent Technologies, USA). Library preparation was done using Illumina Small RNA library prep kit, according to manufacturers protocol.