Nanopore sequencing of mock sample:Routine
Genomic DNA were extraction from HEK293T cells with DNeasy Blood & Tissue Kit (Qiagen, 69504); microbial genomic DNA of Staphylococcus epidermidis, Rosemonas mucosa, Staphylococcus hominis and Staphylococcus aureus were extraction with QIAamp DNA Microbiome Kit (Qiagen, 51704); genomic DNA of other microorganisms were extracted by Rapid Bacterial Genomic DNA Isolation Kit (Sangon Biotech, B518225-0050). All procedures were carried out according to the manufacturer instructions. The high molecular weight DNA (> 10 kb) were enriched through low concentrate agarose gel (0.6%) with TBE buffer (0.5%)(0.5). DNA concentrations were determined using the Qubit dsDNA HS (High Sensitivity) assay kit (ThermoFisher, Q32851); DNA purity was measured by NanoDrop based on the assessment curve shape and the ratio of OD 260/280 and OD 260/230. DNA fragment distribution was determined by Qsep100 (Bioptic) with S3 Cartridge (Kilo Base Cartridge, C105106).In this sample, it was sequenced by nanopore sequencing routine setting.