Tn-Seq of bacteria:Streptococcus agalactiae A909
The GBS A909 library was serially diluted 1:10 three times and plated respectively on THB agar with Km and 1 mg/ml bile salts in two 245 cm2 square plates with 8  107 colonies each (~100 coverage TA dinucleotides site of the genome) and incubated for 12 hours. After the three replicates, all bacteria were harvested for genomic DNA extraction.