NextSeq 500 sequencing; Reconstructing the RUNX2 transcriptional hierarchy in thyroid cancer using a multi-layered network- based approach (ChIP-seq Histone Markers)
Reconstructing the RUNX2 transcriptional hierarchy in thyroid cancer using a multi-layered network- based approach (ChIP-seq Histone Markers)
20M cells were collected for each IP. Cells were cultured in DMEM medium supplemented with 10% FBS and 1% P/S. ChIP samples were purified with PCR Purification Kit (Qiagen) and eluted in 50ul of Elution Buffer. Libraries were obtained starting from 10ng of ChIP and Input samples following the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs) protocol.