GSM7220679: 110650_2_S28; Homo sapiens; RNA-Seq
In DAISY, venous blood samples are collected at 9, 15, and 24 months and yearly thereafter. Beginning in November of 2007, RNA samples were collected from all DAISY participants with Tempus tubes (Applied Biosystems). We selected samples from two visits (termed IA-1 and IA-2) that occurred after the onset of IA for mRNA sequencing. The IA-1 visit was selected as the first available visit after the onset of IA for all groups. In the progressor group, the visit that preceded clinical onset of T1D was selected as the IA-2 visit. In the maintainer and reverter groups, the IA-2 visit was selected as the visit where age was closest to median age in the progressor group at the IA-2 visit. Total RNA was isolated and purified from frozen (−80°