Maternal ketosis and offspring gut microbiome
Fecal sample collected from neonatal dairy calves born to dams diganosed with ketosis. Sample kept at -80C until microbial genomic DNA extractions. Microbial genomic DNA was extracted using repeated beating followed by Qiagen stool kit. PCR amplification of bacterial V1-V3 regions of 16S rRNA genes. Sequencing using Illumina MiSeq2X300.