GSM7732827: MG1655 invitro N-lim E phase RNA_biol rep 2; Escherichia coli str. K-12 substr. MG1655; RNA-Seq
All in-vitro RNA samples were extracted using the Qiagen RNeasy Mini Kit, following manufacturer's protocol. For in vivo samples, RNA samples were extracted using phenol chloroform extraction method. DNase treatment was done using DNAse I enzyme (Invitrogen, Cat no. AM2238). The samples were quantified using the Agilent Bioanalyzer (Agilent, Catalog no. G2939BA). Approximately 100 ng of total RNA from each sample were processed at University of Bern for ribo-depletion with the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina, Catalog no. 20037135). RNA libraries were prepared using the TruSeq Stranded Total RNA Library Prep kit. Libraries were sequenced on a NovaSeq™ 6000 system, using 1×100 bp single-end read length.