African American adolescent idiopathic scoliosis
LIBRARY- Automated dual indexed libraries were constructed with ~100-250 ng of genomic DNA utilizing the KAPA HTP Kit (Roche Diagnostics, Cat #7962363001) on the SciCloneG3 NGS instrument (Perkin Elmer) targeting 250 bp inserts.  Libraries were pooled at equimolar ratio yielding 5µg library pools.  Each library pool was hybridized overnight with the xGen™ Exome Hyb Panel v1 (IDT, Cat #1056115)(IDT Technologies) that spans 39 Mb target region (19,396 genes) of the human genome. SEQUENCING- The molarity of each library capture pool was accurately determined through qPCR utilizing the KAPA library Quantification Kit (Roche Diagnostics, cat #7959478001, #7960085001) to produce cluster counts appropriate for the Illumina NovaSeq4000 instrument.  Normalized library pools were sequenced on a NovaSeq4000 Flow Cell (HCS v3.3.52) and a PE 151 cycle sequencing recipe according to manufacturer protocol targeting 6Gb per library.