GSM8194852: SN_noSNCA_noDBS_r19; Rattus norvegicus; RNA-Seq
After the animals were sacrificed the SN was microdissected and RNA isolated using the RNeasy micro kit (Qiagen #74004) according to manufacturer's instructions. Library preparation was performed by using the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara Bio, Kusatsu) Single-end sequencing with a read length of 75nt in was performed on an Illumina NextSeq 500 system (Illumina, San Diego, CA, USA)