Illumina HiSeq X paired end sequencing; Apelin-VEGF-C mRNA delivery as therapeutic for the treatment of secondary lymphedema
Apelin-VEGF-C mRNA delivery as therapeutic for the treatment of secondary lymphedema
Total RNA from HDLEC treated or not with conditioned media containing APLN were harvested 24h after treatment Total RNA was isolated using the RNeasy mini kit (Qiagen). DNA digestion was performed using the RNase-Free DNase set (Qiagen) Library was constructed by GeneWiz corporation according to their pipeline.