RNA-seq of human adult aortic valves
Libraries were prepared using poly(A) and NEB Library Prep Kit for Illumina to select stranded mRNA (New England Biolabs, E7420L). Expression was assessed by 150bp length paired-end RNA-sequencing (RNAseq) on a NovaSeq 6000 instrument (Illumina), aiming for ~50 million paired reads per sample.