GSM8062175: C05_spegJY044_FANCF_5GtoT_6U_rU_donor4_PEmax; Homo sapiens; OTHER
Genomic DNA was extracted using the DNeasy Blood & Tissue Kit (Qiagen 69506) per manufacturer's instructions with an elution volume of 100 µL To assess editing efficiency, PCR was performed with 25 uL of eluted genomic DNA per sample in an 100 µL PCR reaction with KAPA HiFi HotStart ReadyMix (Roche 9420398001) with the following cycling conditions: 95 C for 3 min, 28 cycles of [98 