RNA-Seq of Columba livia: HH25 embryo head
RNA from the whole head of a single HH25 Homer/Parlor roller mix embryo was extracted using the QIAGEN RNEasy Plus Kit. cDNA was synthesized and amplified using the NEBNext Low Input cDNA Synthesis and Amplification Module (E6421). This cDNA was used to generate a PacBio SMRTbell adapted library and sequenced on the Sequel II platform.