scRNA-seq of mus musculus: UCMS mice femur CD45 cells
Bone marrow cells were stained with FITC anti-mouse Ter119 antibody, APC anti-mouse CD71 antibody, and PE-cy5 anti-mouse CD45, incubated on ice for 30 minutes in PBS containing 0.1 % BSA, then washed twice in the dark, resuspended in PBS with 0.1 % BSA containing 7AAD (2 l/ml, Sigma-Aldrich), and sorted on BDFACSAria III (BD Biosciences), and the purity of sorted populations was verified to be greater than 90 % by flow cytometry analysis.