PacBio: assessing AAV genome integration after Cas9 dsDNA break in mouse brain
Primers unique to the TdTomato locus were used to amplify genomic DNA from mouse brains and append sample-specific barcodes. The resulting 1100 bp amplicon was submitted to the QB3 Vincent Coates Genomic Sequencing Lab for library prep and sequencing on a PacBio Sequel 8M SMRT Cell, yielding approximately 110,000 reads per sample.