WGS of  Popillia japonica larvae gut
For whole metagenome sequencing, the DNA was first analyzed to ensure that the samples had sufficient integrity and concentration for optimal library preparation. As a standard procedure, 1 L of gDNA from each sample was used to test integrity and purity by 1.5% agarose gel electrophoresis. Subsequently, a Qubit fluorometer was used to quantify DNA and determine whether it was sufficient to proceed; the integrity of this DNA was also verified. Thereafter, the samples were used for library preparation and sequencing. The generated DNA fragments (DNA libraries) were sequenced using an Illumina Novaseq platform, using 150 bp paired-end sequencing reads.