MinION metagenome of pooled plastisphere DNA for optimisation of multi-omics and metaproteomics of the marine plastisphere.
Library preparation for metagenomic sequencing on the Oxford Nanopore MinION Mk1c included DNA end repair (NEBNext ONT Companion Module) and barcoding (Native Barcoding Kit 24; SQK-NBD112.24). Approximately 5fmol of the resulting library was loaded onto the ONT MinION (Mk1C; FLO-MIN106) following the standard operating procedure. Sequencing was performed for 24 hours with the following parameters established in MinKnow (v22.10.5): discard reads < 200 bp and quality scores < 7, with basecalling in real-time using Guppy (v6.3.8).