GSM7061710: PFG_DIP-seq_Input; Plasmodium berghei; OTHER
The recombinant AP2 domain of AP2-FG was prepared as a GST-fused protein. This recombinant GST-fused AP2 domain of AP2-FG and genomic DNA fragments were incubated at RT for 30 min in 100 μL of binding/washing buffer. The mixture was then incubated with 50 μL glutathione sepharose resin (Cytiva) at RT with rotation. Resin was washed three times with 150 μL of binding/washing buffer, and bound protein was eluted with 10 mM glutathione solution. Input genomic DNA fragments were also sequenced as a control. Recovered DNA (5 ng) was used for library preparation.  Libraries were constructed using KAPA Hyperprep kit (KAPA Biosystems).