GSM8112109: GEX15; Homo sapiens; RNA-Seq
Single-cell suspensions were obtained from cell cultures using Accutase cell dissociation reagent (Stempro) Single-cell suspensions were barcoded using oligo-conjugated lipids following the MULTI-seq workflow and frozen live for G1-G13 (note, G2 was removed due to a technical failure), or frozen live and barcoded after thawing using the CELLPLEX (10x Genomics) workflow for G14-G27. After thawing cells were stained with DAPI. A maximum of 10,000 live cells per sample were sorted with a FACS-Aria v3 and pooled in sets of 3 or 4 samples by differentiation stage (from 3-5 independent replicate differentiation experiments). Each pooled group was processed using the 10X Genomics Single Cell 3' v3.1 workflow following the manufacturer's instructions. Enriched barcode libraries were indexed following the MULTI-seq workflow. After quality control, libraries were sequenced on the Illumina NovaSeq S4 (G1-13) or S2 (G14-27) platform in 2x150bp paired-end mode. Supplementary Table 1 includes an overview of sequencing data and performance metrics.