GSM7851395: brain_brat_IgG_rep2; Drosophila melanogaster; OTHER
CUT&RUN was performed using EpiCypher reagents according to the manufacturer protocol. 50 brains were used for each CUT&RUN reaction. Overnight incubation with antibodies was performed in antibody buffer containing 0.05% digitonin. 0.5 µL anti-Zld antibody was used for CUT&RUN. Libraries were prepared using the NEBNext Ultra II kit (NEB). During library preparation, cleanup steps were performed using a 1.1X ratio of Axygen paramagnetic beads, as recommended by EpiCypher. PCR amplification was performed with the following conditions: 98°C for 45 seconds, then 14 cycles of 98°C for 15 seconds, 60°C for 10 seconds, and a final extension of 72°C for 1 minute.