GSM7912319: POD23R; Homo sapiens; RNA-Seq
RNA was extracted from FFPE samples with RNeasy FFPE kit from Qiagen, Part# 73504. NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760, New England BioLabs, Ipswich, MA) was used for sample library preparation according to the manufacturer's instruction.  PCR enrichment of adaptor ligated cDNA were pooled and hybridized with probes from Twist Exome Kit #2 (Twist Bioscience, San Francisco, CA). After post capture PCR amplification and purification, the quality of final libraries was checked and loaded to Illumina NovaSeq6000 for sequencing at PE2x100 cycles