MiSeq of retained phage sequences
Three proteins affinity selected phage displaying seed mRNA based on the translation product. Phage displayed protein fragments were from polyA mRNA from quiescent, 12-, 24-, and 36-hour imbibed seeds that had not yet completed germination. Bar codes for demultiplexing the reads are: Soybean GmPM12 Rep1: CCC, Rep2: CGG, Rep3: AAC; Arabidopsis LEA14 Rep1: AGC, Rep2: AGA, Rep3: ACG; BSA Rep1: CCG, Rep2: CAA, Rep3: CAG. All others not part of this study.