GSM8151564: UK5136_AD; Homo sapiens; RNA-Seq
We used the laser-capture RNA-seq method to profile total RNA of brain neurons similar to what we reported in previous studies. In brief, laser-capture microdissection was performed on human autopsy brain samples to extract neurons. For each temporal cortex (middle gyrus) about 300 pyramidal neurons were outlined in layers V/VI by their characteristic size, shape, and location in HistoGene-stained frozen sections and laser-captured using the Arcturus Veritas Microdissection System (Applied Biosystems) as in previous studies. Total RNA was isolated and extracted with DNase using the Arcturus Picopure method Libraries were constructed using the TruSeq RNA Library Prep Kit v2