GSM8409606: Ctrl4; Mus musculus; RNA-Seq
Mice were i.p. with 1.5mL of 3% thioglycollate.3 days later, mouse were sacrificed, peritoneally injected with 10mL PBS and the peritoneal exudate was collected 5 minutes later. The cells were collected by 800×g, 10 minutes centrifugal and for RNA isolation with TriZOL. RNA libraries for RNA-seq were preparing with SMARTER mRNA-seq Library Pre Kit following manufacturer's protocols.