GSM6603517: 18674X1 empty vector; Mus musculus; RNA-Seq
CD4+ T cells were purified from female Ly5.1 SMARTA and Ly5.1/5.2 SMARTA mice, and separately transduced with pMIG-OCA-B or empty vector (EV). On the following day, transduced GFP+ cells were isolated by FACS and 20,000 of each combined 1:1, and injected intravenously into C57BL/6J male recipients. 24h later, each recipient mouse was injected intraperitoneally with 2x10E5 pfu LCMV-Arm. After eight days, splenic CD4+ T cells were purified using a Miltenyi CD4 T cell isolation kit. Cells were stained with PerCP-Cy5.5 Ly5.1 and AF700 Ly5.2 antibodies. GFP+Ly5.1+Ly5.2+ (EV) and GFP+ Ly5.1+ (OCA-B-expressing) cells were sorted by fluorescence-activated cell sorting (FACS) and deposited for single-cell RNA sequencing. 10X Genomics Single Cell 5' Gene Expression library prep