SC5c
DNA was extracted using a ZymoBiomics DNA/RNA MiniPrep Kit and amplified with primers adapted from Parada et al. (2015) as described in Stamps et al. (2016); barcoded, purified and pooled amplicons sequenced on an Illumina MiSeq at the Genomics and Microarray Core at the University of Colorado Anschutz Medical School ; v2 paired-end 2 x 250 bp chemistry.