RNA-Seq of freshwater microbial biofilms
Sample 16S rRNA gene amplicon libraries were prepared and sequenced by the University of Delaware Sequencing & Genotyping Center using primers 515F (Caporaso et al. 2011) and 805R (Herlemann et al. 2011) (V4 region) and MiSeq PE 300bp sequencing (Illumina, San Diego, CA, USA). The PCR thermocycler conditions were 95C for 3 minutes, 25 cycles of: 95C for 30s, 60C for 30s, 72C for 30s, 72C for 5 minutes.