GSM7733873: Day 3 Null, Day3 Cre+, Day 14 Null, and Day14 Cre+ GEXPool; Mus musculus; RNA-Seq
To recover CD45+ cells from the soft tissue surrounding the infected joint by FACS, tissue homogenates were filtered, and red blood cells (RBCs) were eliminated by rapid lysis in H2O, with isotonic conditions returned with the addition of 10X PBS. Next, cells were incubated with TruStain FcX (BioLegend) prior to staining with CD45-APC (RRID: AB_312977), Ly6G-PE (RRID: AB_1186099), CD11b-FITC (RRID: AB_312789), F4/80-PE-Cy7 (RRID: AB_893490), and Ly6C-PerCP-Cy 5.5 (Cat. #560525; BD). Dead cells were excluded from analysis using a Zombie UV fixable Live/Dead stain (Cat. #423108; BioLegend).