NGS of PCR products encompassing the DSB of the Abracl loci
Libraries were prepared using the Ion Plus Fragment Library Kit (Thermo Fischer Scientific). The quantification was performed by qPCR using the Ion Universal Library Quantitation kit (Thermo Fischer Scientific) and template preparation was carried out on an Ion Chef System (Thermo Fischer Scientific). Sequencing was performed on an Ion Torrent GeneStudio S5 (Thermo Fischer Scientific).