RNA-seq of Schmidtea mediterranea S2F18: whole wild type worms and pooled regeneration series (over 7d)
Total RNA extracted from snap-frozen planarian tissue using the protocol described in Liu, SY. and Rink, J.C. (2018). RNA was purified using a Clean & Concentrator-25 kit (Zymo). The poly-A+ fraction of RNA was isolated using Oligo d(T)25 Magnetic Beads (New England BioLabs Inc.). The resulting poly-A+ RNA fraction was then purified again on a Clean & Concentrator-5 Column Kit (Zymo). The direct RNA libraries for Oxford Nanopore Sequencing were prepared using the SQK-RNA002 kit, starting from 4ng of poly-A+ RNA. Sequencing was performed on a FLO-PRO002 R9.4.1 flow cell.