ATAC-seq
mESCs were transfected with DuxWt, DuxMut and empty plasmids, respectively, and induced with doxycycline (4ug/mL) for 24 hours. And cells were collected for ATAC-seq (TransNGS, KP171-01). All libraries were sequenced on the Illumina NovaSeq 6000 platform in 150-bp, paired-end format. Paired-end, raw sequencing reads were subjected to quality control and adapter trimming using the Trimmomatic(Version 0.40).  The high-quality reads were aligned to the UCSC mm10 build using the Bowtie2 aligner (version 2.4.5), utilizing the parameters --end-to-end, --very-sensitive, and --no-mixed to ensure stringent alignment criteria. Picard(version 2.25) is used to remove duplicates in bam files, and then samtools(version 1.9) is used to extract results with MAPQ values greater than 30 for high-quality comparison results. Samtools was used to index the results. Deeptools(version 3.5.5)  was used to  obtain bigwig files and calculate the enrichment of ATAC.