RNA sequencing of mosquitoes collected in Cambodia
Nucleic acid extraction was with NucleoMag Pathogen kit (Macherey-Nagel), followed by Turbo DNase (Ambion) treatment. rRNA depletion was based on RNAse H with custom probes to target mosquito rRNA. cDNA synthesis was randomly primed. Sequencing library preparation with Nextera XT DNA Library Preparation Kit (Illumina). 2 x 150 cycles.