GSM7988979: 0 uM NIMP 2; Homo sapiens; RNA-Seq
Co-cultures were treated with NIMP (0, 4, and 100 micromolar) for 24 hours, and cell were lysed at 6-days post exposure using RLT buffer containing β-mercaptoethanol, and the supernatant collected. Total RNA was extracted and purified from the collected supernatant using the RNAeasy mini spin columns (Qiagen). Poly(A)+-enriched cDNA libraries were generated using the Illumina TruSeq RNA Library Prep kit v2 (Illumina Inc., Hayward, CA, USA).