RNA-Seq of Calyptocephala attenuata whole body
Whole body (internal organs) were dissected and snap-frozen in liquid nitrogen. Total RNA was extracted using the QIAGEN RNeasy Mini Kit with DNase treatment. RNA was quantified using the Qubit RNA High Sensitivity (HS) kit. 600 ng of total RNA were used as input for RNA sequencing library preparation. mRNA enrichment was performed using the NEBNext Poly(A) mRNA Magnetic Isolation Module and RNAseq libraries were constructed using the NEBNext Ultra Directional II RNA Library Prep kit.