GSM7882422: Eimeria maxima APU-1 T0, replicate B; Eimeria maxima; RNA-Seq
Total RNA was extracted from frozen oocyst Trizol pellets.  Oocysts were thawed and ground with a Teflon pestle. Homogenates werre mixed with chloroform and centrifuged at 12,000 g for 15 minutes at 4°C. The upper aqueous phase was transferred to new tubes and mixed with 70% ethanol. Total RNA was then isolated using a Qigaen RNeasy kit following manufacturer's instructions. Total RNA was quantified using a ThermoFisher Scientific Qubit 3.0 fluorometer and quality was assessed using an Agilent Bioanalyzer 2100. RNA samples were frozen at -80°C. Prior to RNA-Seq, DNA was removed from total RNA with an Invitrogen Turbo DNA-free kit. Libraries were produced from 500 ng of total RNA from biological replicates using an Illumina stranded mRNA Prep Kit (ligation). Bioanalyzer analysis determined the average library size ranged 302-388 bp