GSM7988844: scATAC-seq_M22_rep1; Rattus norvegicus; ATAC-seq
Mammary glands were collected freshly and digested in digestion medium containing 2 mg/mL collagenase type IV, 2 mg/mL hyaluronidase and 2 mg/mL BSA in DMEM/F12 medium, at 37 ºC for 1 to 2 hours, then viable single cells were submitted to Center for library construction. Libraries were constructed using the 10X Genomics ATAC-seq kits v2 by Functional Cancer Epigentics in Dana-Farber cancer institute.