GSM8324531: KPC689 resistant Vehicle; Mus musculus; RNA-Seq
Pancreas/tumors were digested in 4 mg/mL collagenase IV and 4 mg/mL dispase. Samples were stained with eFluor780 Fixable Viablity dye and live cells sorted. 5,000 to 10,000 cells were loaded 10x Chromium Controller or Chromium X to generate Single Cell 3' Gene Expression dual indexed libraries (10x Genomics). The creation of Gel Bead-In-Emulsions (GEM) and subsequent cell barcoding, GEM-RT cleanup, cDNA amplification, and library assembly were performed with the Chromium Next GEM Single Cell 3' Reagents Kits v3.1 (10x Genomics) according to manufacturer's instructions.