gDNA HiSeq 4000 of Anopheles aquasalis single female
The library was prepared using the Nextera DNA sample preparation kit (Epicentre Biotechnologies, Madison, WI), with amplification step (5 cycles) as outlined in the Nextera protocol. The fragment size distribution was analyzed utilizing a 2100 Bioanalyzer with a 7500 DNA assay kit (Agilent Technologies, Santa Clara, CA). Fragments of ~600 bp long were carried out for sequencing. The library was sequenced on one lane of an Illumina HiSeq2000 instrument to generate paired-end reads. Sequencing was performed by The Vincent J. Coates Genomics Sequencing Laboratory (GSL) at the University of California, Berkeleyand sequenced with Illumina HiSeq 2000 technology