GSM8425610: Mesenchymal stem/stromal cells treated with 100 ng/mL Pam2CSK4 for 24 hours; Homo sapiens; RNA-Seq
Cell suspensions were loaded with the reverse transcription master mix onto 10x Genomics Single Cell Chip G, where cells were partitioned into the GEMs along with gel beads coated with oligonucleotides. These oligonucleotides facilitated mRNA capture inside the droplets by 30 bp oligo-dT after cell lysis and provided barcodes to index cells (16 bp) as well as transcripts (12 bp UMI). Following reverse transcription, cDNAs with both barcodes were amplified, and libraries were compiled using the Single Cell 3' Reagent Kit for each sample. Following reverse transcription, cDNAs with both barcodes were amplified, and libraries were compiled using the Single Cell 3' Reagent Kit for each sample. The libraries were then sequenced on an Illumina NovaSeq 6000 System ((Illumina, San Diego, CA) in the 2 × 150 bp paired-end mode.