GSM8195555: Stromal cells of Nras* MBMs in mice; Mus musculus; RNA-Seq
After MBM sample preparation as described above, cell suspensions were subjected to FACS isolation using a gating strategy that consisted in selection of a DAPI negative population (live cells). Cells were sorted into individual tubes per group, 15.000 cells per tube and later encapsulated Live cells were encapsulated intro droplets and libraries were prepared using Chromium Single Cell Reagents kit v3 single index according to the manufacturer's protocol (10X genomics, PN-1000269).