RNA-Seq of Human mesenchymal progenitor cells
Libraries were prepared using MGIEasy and mRNA library preparation chemistry systems. Sequencing was performed on an MGITech MGISEQ2000RS system, with V2 MGIEasy chemistry, on three sequencing lanes with at least 400 million raw reads per lane. Read sequences were output as FASTQ, which were mapped to a human genome index file downloaded from the University of California, Santa Cruz (UCSC) genome browser in March 2021 with the package 'Rsubread, on R version 4.0.5 using the RStudio software (version 1.4.1106). Mapped read sequences underwent quality control, with Phred quality scores greater than 30 (1 in 1000 chance of incorrect base call) deemed adequate.