RNA-seq of sorghum: root
Samples were harvested and frozen immediately in liquid nitrogen. Total RNA was extracted using TRNzol Universal Reagent (TIANGEN). mRNA was purified by using oligo(dT) and then fragmented by incubating in fragmentation buffer. The fragmented mRNA was primed with random hexamer primers and reverse-transcribed with Reverse Transcriptase. After end repair, adenylation, adaptor ligation, purification,PCR amplification, and quality control, sequencing was performed on an Illumina HiSeq.