Doliops shotgun wgs
DNA was sheared using a Covaris M220 (Covaris Inc., USA). Libraries were constructed with the NEBNext Ultra-II DNA Library Preparation kit (New England Biolabs Inc, USA) following the manufacturers protocol. To minimize PCR replicates in the final dataset, we titrated the number of PCR cycles as described in. The average sizes of the final libraries were around 250 bp to 400 bp.