GSM7733651: K562 cells, DRB, Input; Homo sapiens; ChIP-Seq
Cells were crosslinked with 1% formaldehyde for 10 min at room temperature. Pellets of 1x106 cells were used for immunoprecipitation, lysed for 10 min on ice. Chromatin was fragmented using a Branson 250 digital sonicator. Each ChIP was performed with 4ug of antibody (H2A.Z (Abcam ab4174, lot GR3176820-1), and IgG (Abcam ab171870)), incubated overnight at 4°C. A 50/50 slurry of protein A and protein G Dynabeads was used to capture enriched chromatin, which was then washed before reverse-crosslinking and proteinase K digestion at 65C. BGISEQ platform Chip-seq library preparation