RNA-seq of Ctenoides ales mantle tissue dark
Flash frozen whole-animal tissue was stored at -80C until preparation for RNA isolation. . Isolated tissue was first ground into a homogenous powder using an OPS Diagnostics LLC CryoGrinderTM System and then stored at -80C until RNA isolation. Total RNA was isolated from ground tissue powder using an E.Z.N.A Total RNA Kit II (Omega BIO-TEK). RNA purity was assessed with Nanodrop. RNA integrity and quantity was determined with an Agilent 2100 Bioanalyzer (Agilent Technologies). Libraries for sequencing were prepared with the NEBNext Ultra Directional RNA Library Prep Kit (Illumina), following poly(A) mRNA enrichment with Oligo dT beads.