GSM6603330: garlic-derived exosomes; Allium sativum; miRNA-Seq
After total RNAs were extracted from GDE  by the exoRNeasy Maxi Kit (Qiagen), and the quality and concentration of the RNAs were checked using an Agilent 2200 UV spectrophotometer. A total amount of 3 μg total RNA per sample was used as input material for the small RNA library. Sequencing   libraries were generated using NEBNext®Multiplex Small RNA Library Prep Set for Illumina®(NEB, USA.)   following manufacturer's recommendations and index codes were added to attribute sequences to each sample.