GSM8111396: ICELL8.GFP.OE.CM; Mus musculus; RNA-Seq
scRNA-seq libraries were generated using the ICELL8 single-cell system (Takara Bio, Japan). Briefly, isolated cells were stained with ReadyProbes® Cell Viability Imaging Kit (Thermo Fisher Scientific, R37610) according to the manufacturer's instructions. The cells were counted after PBS washing. A cell suspension of 2.0×103 cells/ml was transferred to the SmartChipTM MultiSample NanoDispenser (Takara Bio, MSND) for single-cell preparation. Dispensed cells were then imaged on the imaging station, and live single cells were selected based on Hoechst+PI- staining. The reverse transcription and first-step amplification were performed on a Chip Cycler (Bio-Rad), followed by cDNA purification and size selection with Agencourt AMPure XP beads (Beckman Coulter, A63880). One nanogram of purified cDNA was used to generate a sequencing library with the Nextera XT DNA Library Prep Kit (Illumina, FC-131-1024), and then sequenced on the NovaSeq 6000 platform (Illumina) using the 150 bp paired-end sequencing protocol. One nanogram of purified cDNA was used to generate a sequencing library with the Nextera XT DNA Library Prep Kit (Illumina, FC-131-1024) NovaSeq 6000 platform (Illumina) using the 150 bp paired-end sequencing protocol.