Ultra-low amplification of A001 adenocarcinoma DNA
Genomic DNA was sheared to approximately 11 kb with a Covaris g-TUBE following manufacturers instructions. The results of the shearing were evaluated with a Femto Pulse system (Agilent Technologies, Santa Clara, California). For each sample, 10 nanograms of DNA was processed with the theSMRTbell gDNA sample amplification kit following manufacturers instructions (Catalog 101-980-000, PacBio, Menlo Park, California). Libraries were size-selected >8 kb with a Blue Pippin system (Sage Science, Beverly, Massachusetts).