hc-tNGS of Homo sapiens: BALF sample
Libraries were constructed through reverse transcription, cDNA double strand repaired, fragmentation, end-repair, adapter-ligation and PCR amplification. Eight uniquely barcoded libraries were pooled to hybridize and capture by specific biotinylated probes for 0.5 h using the MetaCAP Pathogen Capture Metagenomic Assay Kit (KingCreate, Guangzhou, Guangdong, China). Quality qualified libraries were quantified using Equalbit DNA HS Assay Kit (Vazyme, Nanjing, Jiangsu, China).