GSM6433628: Before gilteritinib; Homo sapiens; ATAC-seq
Cells were lysed and the rearrangement reaction was performed using Tn5 Transposase (Illumina Catalog #FC121-1030) for 30 min at 37°C. The reaction solution was purified with Qiagen MinElute PCR Purification Kit. Next, five cycles of PCR with custom Nextera PCR primers were performed using NEBNext Q5 Hot Start HiFi PCR Master Mix. The number of additional PCR cycles was determined by qPCR of the partially amplified product. PCR products were prepared using Agencourt AMPure XP beads using the manufacturer's The PCR products were purified using Agencourt AMPure XP beads by double size selection (left ratio: 1.4X, right ratio: 0.5X) according to the manufacturer's protocol.