CRISPR/Cas9 miRNA screen of MutuI Burkitt's Lymphoma cells
MutuI cells were transduced with the pCW-Cas9 vector then transduced with LX-miR library. Resulting MutuI-pCW-Cas9-LX-miR cells were grown and FACS sorted based on the presence of EBV gp350. DNA was extracted from the gp350+ and gp350- cell populations and sequencing libraries were made first by PCR amplifying the sgRNA regions and then a PCR on these amplicons using a staggered pooled primer approach to add the Illumina adaptor and index sequences.