GSM7989008: 36N; Homo sapiens; RNA-Seq
Tumors and adjacent colonic segments were surgically resected and aliquots were stored in RNAlater (Thermo Fisher) preservative. Samples were homogenized with a pestle and chloroform was added. The samples were centrifuged at 12,000 x g for 15 minutes. The aqueous layer was purified with RNAEasy Mini Kit (Qiagen). Samples were selected for poly-adenylation, and cDNA libraries were made and sequenced on MiSeq2500 (Illumina). RNA libraries were prepared for sequencing using standard Illumina protocols