GSM7147316: mDC_3; Homo sapiens; RNA-Seq
Total RNA was isolated from cells/tissues using the Magzol Reagent (Magen, China)according to the manufacturer's protocol, The quantity and integrity of RNA yield was assessed by using the K5500(BeijingKaiao, China) and the Agilent 2200 T apeStation (Agilent T echnologies, USA) separately. the RNA fragmentswere subjected to first strand and second strand cDNA synthesis following by adaptor ligation and enrichment with a low-cycleaccording to instructions ofNEBNext® Ultra™ RNA Library Prep Kit for Illumina. The purified library products were evaluated using the Agilent 2200 T apeStation and Qubit(Thermo Fisher Scientific, USA)