GSM8112565: H128 cells, siCont; Homo sapiens; RNA-Seq
sample1-3:Total RNA was isolated using TRIzol reagent according to the manufacturer's instruction (15596026, Invitrogen, USA). sample1-3:DNase I was used for column digestion to ensure RNA quality. RNA library preparation was performed using the NEB Next Ultra Directional RNA Library Prep Kit for Illumina (E7420L). RNA libraries were prepared for paired-end sequencing using standard Illumina protocols.