GSM8112302: GM00637, Wildtype, none, ATACseq control, gDNA sequencing; Homo sapiens; ATAC-seq
Fibroblasts were first trypsinised (Sigma-Aldrich, Cat. #SLCH3385), pelleted at 300 g for 5 min and washed once with cold 1x Dulbecco's phosphate buffered saline (PBS, Gibco, Cat. #14190). 50, 000 cells were subjected to ATACseq preparation essentially as described in Corces, M., Trevino, A., Hamilton, E. et al. An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues. Nat Methods 14, 959–962 (2017). https://doi.org/10.1038/nmeth.4396