GSM7062261: H3K27ac,control, MDA231, rep2; Homo sapiens; ChIP-Seq
Cells were fixed with 2 mM Di(N-succinimidyl) glutarate for 45 min at RT and with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using the Diagenode Bioruptor 300. ChIP-seq libraries were prepared using ChIPSeq Illumina protocol. Libraries were validated using the High Sensitivity D5000 ScreenTape (Agilent Technologies).