O. camponoti floridani strain Arb2 in liquid culture sampled at ZT22 under 12:12 LD
We first extracted total RNA from the frozen fungal sample, then isolated mRNA with poly-A magnetic beads (NEB) from 500 ng total RNA. Next, we converted purified mRNA to 300 bp fragment DNA libraries with the Ultra II Directional kit (NEB) and indexed samples for multiplexing (NEB)