GSM7818243: SF11411_T0_Rep3; Homo sapiens; OTHER
gDNA were extracted using Quick-DNA Miniprep Plus Kit (Zymo Research #D4068). NGS libraries were constructed using a two-step PCR protocol. In the first step, PCR was conducted with primers containing partial Illumina adaptors to amplify the sgRNA cassettes. Subsequently, a second PCR was performed to attach full Illumina adaptors and barcode the samples.