GSM8324304: B12 High treated splenic CD11b+ Cells; Mus musculus; RNA-Seq
Viably frozen single cell suspensions of splenic cells were FACS sorted on CD45.2+CD11b+DAPI. Cells were resuspended in 10% FBS in PBS. Library preparation was performed according to manufacturer's instruction (single cell 3' v2 protocol, 10x Genomics).