ONT long reads of Baronia brevicornis
Extraction were done with the Qiagen genomic DNA kit to obtain a better 260/230 ratio as estimated with Nanodrop assays guaranteeing DNA purity for long-read sequencing with Oxford Nanopore Technology (ONT). Final DNA purity and concentrations were measured using both Nanodrop (Thermo Fisher, USA) and Qubit (Thermo Fisher, USA). Whole-genome libraries were constructed using the resulting high-molecular-weight DNA as input for the Nanopore LSK-109 ligation kit (Oxford Nanopore Technologies, UK) following the manufacturers protocol. Long-read sequencing was performed on a GridION device with three R9.4.1 flow cells.