GSM7754661: RV_1; Mus musculus; RNA-Seq
Right ventricular tissue of mouse pups on postnatal day 21 were rapidly removed from executed mouse and flash frozen in -80℃ refrigerator. Samples were rinsed using PBS (calcium-magnesium-free), minced and transferred to collagenase-containing medium for 30 min of digestion. Resuspend the digested cells and enrich the CD45+ positive cells with CD45+ magnetic beads. The cell suspension was loaded into Chromium microfluidic chips with 3' v3 chemistry and barcoded with a 10× Chromium Controller (10X Genomics). RNA from the barcoded cells was subsequently reverse-transcribed and sequencing libraries constructed with reagents from a Chromium Single Cell 3' v3 reagent kit (10X Genomics) according to the manufacturer's instructions. Sequencing was performed with Illumina Hiseq PE150 according to the manufacturer's instructions (Illumina).