Illumina HiSeq 2500 sequencing; Transcriptomic response of Dickeya dadantii to topoisomerase I inhibition by seconeolitsine
Transcriptomic response of Dickeya dadantii to topoisomerase I inhibition by seconeolitsine
Cells were collected in exponential phase (OD=0.2 at 600nm) or transition to stationary phase (OD=1.1 at 600nm).  Growth in minimal medium M63 supplemented with sucrose (0.2% wt/vol) Treated samples were collected 15 min after treatment with seconeolitsine diluted in DMSO (50 uM final concentration). Total RNA from each D. dadantii sample was isolated using the frozen acid phenol method (Maes et Messens 1992), and submitted to the Montpellier MGX Platform for ribosomal depletion (Illumina RiboZero kit) and RNA fragmentation. Strand-specific cDNA libraries were constructed by Montpellier GenomicX MGX Platform.