16S amplicon sequencing of wastewater  effluent
1 L samples of wastewater were collected from influent, secondary effluent and final effluent. Up to 400 mL were filtered through a 0.45 um cellulose filter until clogged (<1 drop every 3 seconds). DNA was extracted from filters using the FastDNA Spin Kit for Soil. For biosolids samples, DNA was directly extracted from 0.5 g samples of biosolids using the same extraction kit. All libraries were prepared using the Illumina 2-step PCR protocol, and sequenced on the Illumina MiSeq 2500.