colon microbiome of Rhinella marina at Middle Point, NT, Australia
Toad raised in semi-natrual, dirty environment. Colon of toad was removed after ~1 month infection with Rhabdias lungworm. Sample was held in -20C until DNA extraction with Norgen's Microbiome kit. Amplicons of the bacterial V4 region of 16S rRNA gene were prepped and sequenced on MiSeq at Ramaciotti Centre for Genomics.