Soil after ADM treatment using biochar-Mn
Genomic DNA was isolated from 1 g of sediment according to a previously described protocol (https://doi.org/10.28940/terra.v39i0.887). The extracted DNA was subjected to gene-based amplification of 16S rRNA using next-generation sequencing performed with the 16S Metagenomic Sequencing Library Preparation (Illumina), targeting the V3V4 region of the 16S rRNA gene. The libraries were sequenced using the Miseq System (Illumina).