RNA-Seq from leaves of tomato with normal watering and mock treatment
The mRNA was isolated using streptavidin-coated magnetic beads and was subsequently fragmented. cDNA was synthesized using random primers and reverse transcriptase SuperScript II from Invitrogen. Double-stranded DNA was subjected to A-tailing and ligation of the barcoded Truseq adapters.  Libraries were prepared using the TruSeq Stranded mRNA Sample Prep Kit v2 according to the manufacturer's protocol. Libraries were loaded at a concentration of 18 pM onto the flowcell, and were sequenced 1 x 50 bp on Illuminas HiSeq 2500.