Whole genome sequencing Suppressor 2S8
dacA1 cells were grown overnight in SF medium, 1mL of overnight culture was spread on a BioAssay Square Dish (Thermo Scientific Nunc) containing 200 mL of LB medium with Streptomycin 200 g/mL. This experiment was performed 3 independent times, for each instance, 16 colonies were selected from the plate, to validate the stability of the mutations each colony was grown overnight in salt-free LB and then in LB agar. Genomic DNA from each selected colony was extracted and samples were sent to SeqCenter (Pittsburg, PA) for whole genome sequencing and variant calling analysis.