GSM6603262: SCI, 2; Rattus norvegicus; RNA-Seq
Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Agilent 2100 Bioanalyzer and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0. Poly(A) RNA was purified from total RNA(1ug) using poly-T oligo-attached magnetic beads using two rounds of purification. Following purification, the poly(A) RNA was fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the TruSeq Stranded mRNA Library Prep Kit(Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (±50 bp).