Metagenomic shotgun sequencing of lake shore sediment: Canadian heavy crude oil, T=44
DNA was extracted from sediment samples (1 g) using the Qiagen PowerSoil kit (Qiagen) and following the manufacturer's protocol. Samples were eluted in 100 L of PCR-grade water and a 20 L aliquot of non-normalized metagenomic DNA was used for sequencing. Sequencing libraries were prepared the Nextera XT Tagmentation kit (illumina).