GSM7851021: TMZ380EA20; Homo sapiens; RNA-Seq
RNA was extracted using the RNeasy MiniPlus kit (QIAGEN) according to the manufacturer's instruction. The amount and purity of RNA was measured by nanodrop as described earlier (37,42). The ratio of absorbance of RNA at 260 nm to the one of RNA at 280 nm was used as indicator of RNA purity. All samples were between 1.99 and 2.05 which accepted as pure. 100 ng/µl were used for the RNA-sequencing. The enrichment was performed by QuantSeq 3´-mRNA Library Prep/ Lexogen