GSM7852815: PCLS_aG1_3; Homo sapiens; RNA-Seq
RNA was isolated using Tri Reagent (T9424, Sigma) and 1-bromo-3-chloropropane (B9673, Sigma) according to manufacturer's instructions. Following Tri Reagent extraction, RNA clean-up was performed using the RNeasy Mini Kit according to the manufacturer's instructions, including on-column DNA digestion. Single-end 75 base-pair libraries were generated using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (015.96, Lexogen) with an RNA input of 100ng. Quality of libraries was then checked using the Agilent TapeStation D1000 ScreenTape (5067-5582, Agilent) and Qubit dsDNA High Sensitivity kit (Q32851, Invitrogen).