GSM7431723: P3_3; Homo sapiens; RNA-Seq
Then total RNA of cells was extracted by Trizol reagent method . Total amounts and integrity of RNA were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA) . RNA libraries for RNA-seq were prepared using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® or NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® following manufacture's protocols.