GSM7431646: Dnmt1_f_f2; Mus musculus; RNA-Seq
Total RNA from the Dnmt1KO and Dnmt1fl/fl peritoneal macrophages was extracted using the RNeasy kit with DNase treatment (Qiagen) and RNA integrity (RIN > 9) was checked by a Bioanalyzer 2100 system (Agilent Technologies). cDNA libraries were constructed using the NEBNext UltraTM RNA Library Prep Kit according to the manufacturer's instructions (Illumina).