Nano-DMS-MaP of HIV-1 genome: in virion 10mM DMS rep2
transfection of plasmid expressing HIV-1 NL4-3 del Gp, 24 h p.tfx. DMS probing, RNA extraction from supernatant, RT with RT-FL, PCR with PCR-HIV_Fw and PCR-HIV_FL_A_RE, and sequencing with native barcoding kit Nanopore SQK-NBD112.96