GSM7073093: Brain_patient_27; Homo sapiens; RNA-Seq
RNAs were isolated using RNeasy kit (Qiagen, 74134) and DNA was removed using RNase-free DNase (Qiagen, 79254). Overall, 500 ng was used as input material for a Low Input Ribozero treatment using the Epicentre Ribo-Zero Gold Kit–Low Input (HMR). The integ The depleted RNA was used as input material for the ScriptSeq v2 RNA-Seq Library Preparation protocol. Following 14 cycles of amplification, the libraries were purified using Ampure XP beads. Each library was quantified using Qubit, and the size distribut