GSM7754980: WT-P4-2; Bombyx mori; RNA-Seq
Total RNA was extracted from tissue samples, and the concentration and purity of the extracted RNA were detected by Nanodrop2000. The integrity of RNA was detected by agarose gel electrophoresis, and the RIN value was determined by Agilent 2100. Single database construction requires a total RNA amount of ≥ 1ug and a concentration of ≥ 35ng/ μ L. OD260/280 ≥ 1.8, OD260/230 ≥ 1.0. The construction and sequencing of the transcriptional library was carried out by Shanghai Majorbio Bio-Pharm Technology Co., Ltd. (Shanghai, China) using the Illumina Novaseq 6000 platform.