GSM6705493: HuT78 ATAC-seq Replicate 2; Homo sapiens; ATAC-seq
Cells suspended in cold ATAC-Resuspension Buffer and incubated on ice for 3 minutes. Nuclei then pelleted at 500 RCF for 10min at 4C. Nuclei resuspended in 50uL transposition mix and incubated at 37C for 30min in thermomixer shaking at 1000RPM. Reactions stopped by adding EDTA and then purified using Zymo DNA Clean and Concentrate kit, and PCR amplified for 5 cycles, and purified with the same Zymo DNA clean and Concentrator kit. Library pool size selected for 100-1000 bp on 1% Agarose gel, and sequenced on Illumina NextSeq2000 for a 100bp pair-ended run to obtain 100 million reads per sample.