cold acclimation 3d #2
Total RNA was isolated from D. antarctica leaf tissues using the RNeasy Plant Mini Kit (Qiagen, Valencia, CA, USA). RNA quality and concentration were conducted using a Bioanalyzer (RIN>6) and a Qubit RNA Broad-range Assay Kit (Life Technologies, Carlsbad, CA, USA). A total of 3 g RNA for each sample was used for construct the libraries using the TruSeq mRNA Library Kit (Illumina, San Diego, CA, USA).