GSM7734336: ctrl_AA14_3; Mus musculus; RNA-Seq
The collected pituitary cells are quickly put into liquid nitrogen and stored at low temperature. TRIzol (Invitrogen, Waltham, Massachusetts, USA) was used to isolate total RNA from pituitary cells according to the manufacturer's protocol. The amount of total RNA was measured with Nanodrop 2000 (Thermo Scientific, Waltham, Massachusetts, USA), and the quality was evaluated by 1% agarose gel electrophoresis, and stained with 4S Red Plus nucleic acid stain (Sango, Shanghai, China). Sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA). Library preparations were sequenced on an Illumina Novaseq platform and 150 bp paired-end reads were generated, which were mapped to the mouse genome using hisat2 (v2.0.0) after quality control filtering.