Long-read whole-genome sequencing of Psychrobacillus sp. L3
Total DNA was extracted from an overnight culture obtained from a single colony of strain L3 using Proteinase K/SDS treatment and Genomic DNA Clean & Concentrator-10 Kit (Zymo Research). Library preparation and the ligation sequencing of gDNA were carried out in accordance with the SQK-LSK109 kit protocol without any deliberate fragmentation or size selection. The resultant library was sequenced using the FLO-MIN106D flow cell (R.9.4.1).