GSM8324755: intestinal organoids from control Adipfl/fl mice(WT); Mus musculus; RNA-Seq
Fresh small intestinal (5–10 cm length) was cut longitudinally, and flushed three times with cold PBS containing penicillin–streptomycin. After the villi were gently scraped with a sterile cover glass, the intestine tissue was cut into small pieces. After 2 mM EDTA incubation for 30 min on ice, crypts were dispersed by pipette, and then filtered using 70 mm cell strainer and collected in a tube, and counted and mixed with Matrigel Total RNAs from cells were extracted according to the manual of TRIzol®reagent (Invitrogen, Shanghai, China)