RNA-Seq of human colon: HCT116 cell
Total RNA samples of HCT116 cells infected with scramble lentivirus vector or LRRC8A lentivirus vector were extracted for RNA-sequencing performed by BioMarker technology company (Beijing, China). Following the quality control of the RNA samples, the mRNA samples were enriched using OligodTbeads and fragmented into approximately 200nt RNA, which were used to synthesize the first strand cDNA and the second cDNA. The purified double-strand cDNA was prepared by end-repair/dA-tail and adaptor ligation. The cDNA libraries were established by PCR amplification and were sequenced on a flow cell using an Illumina HiSeq sequencing platform.