CanPV_CG_WW_AZ_Oct2019
The ~7.5kb amplicons from the cCDS assay were used for library preparation (MinION SQK-LSK110 and EXP-NBD104) following the manufacturers instructions and sequenced on a Flongle flow cell. Base calling was done using Guppy in MinKNOW. Reads were trimmed using Porechop v0.2.4. Subsequently, reads between 6kb to 8kb in length were size selected using Geneious prime v2023. The 6-8kb reads were then assembled and polished using Flye v2.9.1 and Medaka v1.7.2, respectively..