Nano-DMS-MaP of Rnase P RNA component of Thermotoga maritima: IVT 34mM DMS rep2
in vitro transcription of bact. RNase P sequence (with 3' M13 rev adapter sequence), in vitro folding, DMS probing, RNA purification by sodium acetate-ethanol precipitation, reverse transcription with primer M13_pA_re, PCR with primers PCR_bact_RNaseP_fw and M13_pA_re, and sequencing with native barcoding kit Nanopore SQK-NBD112.96