Metagenomic shotgun sequencing of lake shore water: Canadian heavy crude oil, fertilizer, T=95
Water samples (1 L) were filtered using 0.22 m polyethersulfone membranes. DNA was extracted from filters using the Qiagen PowerWater kit following the manufacturer's instructions. Samples were eluted in 100 L of PCR-grade water and a 20 L aliquot of non-normalized metagenomic DNA was used for sequencing. Sequencing libraries were prepared the Nextera XT Tagmentation kit (illumina).