GSM8062565: SU-DIPG-XVII_combine_ATAC; Homo sapiens; ATAC-seq
50,000 cells were used for ATAC-seq. After being washed by PBS, the cells were resuspended in lysis buffer. After spinning down, the pellets of cells were resuspended in transposome mix. The reaction was incubated at 37℃ for 0.5 h in a PCR cycler, before 1 ml 0.5M EDTA, 1 ml proteinase K (20 mg/ml) and 5 ml 10% SDS were added to stop the reaction. After another incubation at 50℃ for 1 h, DNA was purified.