GSM7734284: Liver_HFHF16wk_triplicate 1; Mus musculus; RNA-Seq
Total RNA was harvested using Rneasy Mini Kit (QIAGEN) and was quantified and analysed using Nanodrop spectrophotometer and Bioanalyzer. One µg of total RNA was used for the construction of sequencing libraries, with the service provided by Center for Applied Genomics (Sickkids Hospital, Canada) Libraries were loaded onto Illumina NovaSeq SP flow cells and paired-end sequencing was performed on Illumina NovaSeq SP sequencing instruments.