GSM7598112: Rejuvenation Replicate2; Homo sapiens; Hi-C
Cells were fixed with formaldehyde followed by quenching using 1M Glycine. Fixed cells were then processed for cell lyses using cell lyses buffer followed by restriction digetion with HindIII ovenight. Digested genome was end repair followed by DNA ligation, purification of HiC DNA using ethanol precepitation. HiC libraires were constructed using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina