GSM7789728: shTRPS1 DOX; Mus musculus; ATAC-seq
Orgnaoids were dissociated in TrypLE Express Enzyme (ThermoFisher Scientific). Nuclei were isolated by lysing the cells with chilled lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% Tween 20, 0.1%Nonidet P40, 0.01 % Digitonin, 1 % BSA) on ice for 3 min 30 s. Cells were washed with wash buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% Tween 20, 1 % BSA). Supernatant was discarded and cells were resuspended in 1X Nuclei Buffer (10X Genomics, PN-2000153/ 2000207). 7600 nuclei were loaded onto the Chroimium Controller to recover 5000 nuclei for library prepration and sequencing. 10x Genomics Chromium Controller and Chromium Next GEM Single Cell ATAC Reagent Kits v2 according to the manufacturer's protocol (CG000496)