CRISPR/Cas9 mutation readout on the pathway reporters in validation replicate 1 : Sample from validation experiment treated with DMSO
CRISPR/Cas9 mutation readout on the pathway reporters in different K562 TRIP pools treated with Vorinostat or DMSO (equal volume), 72 hours after activation the cells were collected and processed with two successive PCRs on the integrated pathway reporters to generate the libraries, spanning the sgRNA target site and the barcode