GSM6705683: 2yr Mouse Liver, CgA KO, Replicate 3; Mus musculus; RNA-Seq
Total RNA was isolated from approximately 30 mg of liver tissue from 3 wild type and 3 CgA knockout mice using RNeasy Mini Kit (Qiagen) following the manufacturer's instructions. Total RNA was quantified by nanodrop and was quality checked by Tapestation (Agilent). Poly(A) selected mRNA libraries were prepared from 500 ng of total RNA using KAPA mRNA HyperPrep kit (KAPA Biosystems) and dsDNA libraries were quantified using Qubit 2.0 fluorometer (Invitrogen).