GSM7431418: DELTA-5 HA 24h rep2; Homo sapiens; ChIP-Seq
Cells were harvested after 24 or 48 hours of α-amanitin treatment to perform western-blot analysis. Since Δ5 is unstable after 48 hours of α-amanitin treatment, cells were harvest after 24 hours to perform ChIP using specific antibodies (HA, H3K4me1, H3K4me3 and CTCF) or extraction of Total RNA, PolyA-RNA and chromatin-associated RNA (ChrRNA) using corresponding protocols as described in manuscript. Drosophila cells were added as spike-in for ChIP experiments but not used for normalisation. ChIP-seq libraries were prepared with Illumina's ChIP Sample Prep Kit according to standard protocol. RNA-seq libraries were prepared with Illumina's Small RNA Library Prep Kit by using a modified protocol described in manuscript. HiC libraries were prepared by proceeding to end-repair, A-tailing followed by the ligation of Illumina adaptaters and amplification.