GSM7818045: daf2_hsf1i_2; Caenorhabditis elegans; RNA-Seq
Total RNA in the animals was extracted using RNAiso plus (Takara), and cDNA was synthesized using the ImProm-II Reverse transcription kit (Promega). TruSeq (unstranded) mRNA libraries (Illumina) were constructed and paired-end sequencing of Illumina HiSeq2500 was performed by Macrogen.