NGS of PCR products encompassing the DSB of the Hprt loci
Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina kit (NEB #E7103). The quantification was performed by qPCR using the KAPA Library Quantification kit for Illumina (KK4824). Sequencing was performed on an Illumina MiSeq platform using the MiSeq Reagent Kit v3 (600-cycle) (MS-102-3003).