RNA-seq of Saraca asoca: young bark
mRNA enrichment was done by oligo dT-based mRNA isolation. Intact poly(A)+ RNA was isolated from the total RNA using NEBNext poly(A) mRNA Magnetic Isolation Module. For cDNA library preparation from ribodepleted RNA, NEBNext UltraTM II Directional RNA Library Prep Kit for Illumina was used, following the manufacturers protocol. To create the final cDNA library, the USER digested single strand molecules were enriched and indexed in a limited cycle PCR, followed by AMPure bead purification.