GSM7733075: CTCF ChIP-Seq EF1a Rev #2 rep1; Mus musculus; ChIP-Seq
Prepared 1X10^6 cells in 1ml in 1.5 mL tubes, fix cells in 1% FA at RT for 10 min. (37% FA: 27μl/ml). Added 50 μl 2.5 M Glycine in PBS (final 125 mM) and rotate at RT for 5 min. ChIP-Seq libraries were prepared for sequencing using standard Illumina protocols. Libraries were sequenced on a Nova Seq SP, Single or pair ends