NextSeq 500 paired end sequencing; Identification of CTCF binding sites through CUT&RUN sequencing in the neuroblastoma cell line CLB-GA.
Identification of CTCF binding sites through CUT&RUN sequencing in the neuroblastoma cell line CLB-GA.
0.5M cells were harvested for CUT&RUN. CUT&RUN was performed using Cutana pA/G-MNase (Epicypher, 15-1016) according to the manufacturerâ€™s protocol. Retrieved genomic DNA was purified with the MinElute PCR purification kit and eluted in 10 mL of buffer EB. Sequencing libraries were prepared with the automated Swift 2S system.