LIBRA-seq of human SARS-CoV-2 spike-binding B cells from COVID-19 convalescent individuals
LIBRA-seq of human SARS-CoV-2 spike-binding B cells from COVID-19 convalescent individuals
Blood was collected in Vacutainer CPT tubes (BD), followed by centrifugation at 1800 x g for 20min. PBMCs were washed  with PBS three times and stored at -135 oC. Antigen-specific B cells were purified by staining with fluorescent-labeled DNA-barcoded antigen and antibodies using FACS.     Single-cell suspensions of sorted B cells were loaded onto the Chromium Controller microfluidics device (10x Genomics) and processed using the B cell Single Cell V(D)J solution according to the manufacturerâ€™s protocol. For amplify and purify the antigen barcode libraries, 10x Genomics single-cell VDJ human B cell  assay and target enrichment protocol were completed.