GSM7734232: CCE-Rx, OTX2, ChIP; Mus musculus; ChIP-Seq
Formaldehyde for 10 minutes chromatin prepared, and ChIP performed according to the Upstate (Millipore) protocol, using 10μg of anti-OTX2 antibody (abcam, ab21990-100), anti-SOX2 (Santa Cruz Biotechnology, sc-17320), or mouse IgG (Millipore, PP54) as a control. ChIP-seq libraries were prepared and sequenced using the standard Illumina protocol.