GSM7597471: WC2_S250_L004_R1_001; Mus musculus; RNA-Seq
Brain samples were homogenized in Qiazol buffer (Qiagen, Germantown, MD, USA), and total RNA was extracted using the miRNeasy micro kit on-column DNase (Qiagen) treatment. The NEBNext Ultra II directional RNA library prep kit for Illumina (New Englands Biolabs Inc., MA, USA) was used to prepare mRNA sequencing libraries, according to manufacturer's instruction.