GSM7882417: astrocytes, normoglycemic, rep3; Mus musculus; RNA-Seq
RNA was extracted using Trizol reagent (Invitrogen). Total RNA concentration was calculated by Quant-IT RiboGreen (Invitrogen). TapeStation RNA Screentape (Agilent) was used to assess the integrity of total RNA. Only high-quality RNA preparations, with RIN greater than 7.0 was used for RNA library construction. RNA libraries for RNA seq were prepared using the SMART-SeqTM mRNA Kit, following the manufacturer's protocol.