Con CD45- SVF cells.
SVF were isolated from the iWAT of 4-week-old Lats1fl/fl; Lats2fl/fl; Rosa-LSL-tdTomato (control, Con) and Adipoq-Cre; Lats1fl/fl; Lats2fl/fl; Rosa-LSL-tdTomato (AKO) mice. SVF cells were flow-sorted to exclude CD45 positive leukocytes. Singlets were selected based on gating. CD45-& tdT+ cells from AKO SVF and CD45-& tdT- cells from Con SVF were sorted for single-cell RNA-sequencing.