scDART-seq/scRNA-seq in aged mouse cortex
Mice were placed under anesthesia and perfused with ACSF. The brain was removed, cortex isolated and cut into pieces and digested with Neuronal Isolation Enzyme with Papain (Thermo Fisher). Tissue was gently disrupted with 10mL pipettes to release single cells. Debris was removed by centrifuging for 25 min at 250xg in 30% percoll solution. Cells were counted and viablity measured. Cells were fixed with the Whole Cell Fixation Kit (Parse Biosciences). Split pool barcoding and Illumina library preparation was perfomed using the Whole Transcriptome Evercode Kit (Parse Biosciences)