GSM6798106: APP/PS1, replicate 3, scRNAseq; Mus musculus; RNA-Seq
The left hemisphere of the mouse brain was homogenized to form a single cell suspension, then resuspended and centrifuged in a 37%/70% Percoll Plus gradient at 800G, 23˚C, for 25 min with an acceleration of 3 and a deceleration of 1. Mononuclear cells were taken from the interface layer. The cells were stained with anti-CD11b PECy7 and CD45 PerCP. After staining cells were washed and sorted using BD FACSAriaTM II. Single cell RNA-Seq libraries was generated by the Single Cell Core at Harvard Medical School, Boston, according to the manufracturer's protocol of 10X Chromium Next GEM Single Cell 3' Reagent Kits v3.1