GSM8062068: HUCCT1_CRISPR_Clone_1b; Homo sapiens; RNA-Seq
The analysis of differential gene expression between the HuCCT1 AQP1 KO (Clone 22 )and HuCCT1 WT (clone 1) cell lines was conducted using the RNA-seq technique. Total RNA was obtained using the Qiagen RNeasy kit, following the manufacturer's instructions. RNA libraries were prepared for sequencing using Illumina TruSeq stranded mRNA Protocols at the University of Minnesota Genomic Center (UMGC)