GSM8195046: CARJ-Ramos, scRNA-seq; Homo sapiens; RNA-Seq
Single-cell suspensions were loaded to 10x Chromium to capture 9000 single cell according to the manufacturer's instructions of 10X Genomics Chromium Single-Cell 3'kit (V3) . cDNA was amplified by PCR for 13 cycles and then subjected to size separation by 8% PAGE electrophoresis into >600 nt fragments containing cDNAs reversely transcripted from cellular mRNAs and <300 nt fragments containing barcoded ssDNA labels. The two cDNA fragments were separately converted into sequencing libraries and merged at a desired ratio (50% for ssDNA labels and 50% for cellular cDNA library).