GSM7789725: shRen_rep3_ADT; Mus musculus; RNA-Seq
Mammary gland tissue was dissociated using gentle collagenase/hyaluronidase (Stemcell), mammary epithelial cells were further isolated using 0.25% trypsin  (Gibco) and 5 mg/mL dispase (Stemcell) treatments. Cells were incubated with 0.5 µg of TotalSeq antibodies (anti-EpCAM, anti-CD49f, 118237 and 313633 from Biolegend) 10x Genomics scRNA-Seq was performed using the Chromium single cell 3' assay v3 as recommended by the manufacturer.