GSM7598017: mouse model for HALI, replicate 2; Mus musculus; RNA-Seq
Total RNA was extracted using TRIzol reagent (Magen, China) following the manufacturer's instructions. For each sample, 1 μg of total RNA was used to enrich and purify polyadenylated mRNAs using oligo(dT) beads. The purified mRNAs were then reverse transcribed into cDNA using reverse Rnase H and DNA polymerase I.