RNA-Seq of Neocalanus flemingeri adult females week 0 2021 from PWS2 station 50
Total RNA was extracted from individual adult females (n=4/collection time) using QIAGEN RNeasy Plus Mini Kit (catalog # 74134) in combination with a Qiashredder column (catalog # 79654) and shipped on dry ice to the Georgia Genomics Bioinformatics Core (https://dna.uga.edu). There double-stranded cDNA libraries were multiplexed and sequenced using Illumina Next-Seq 500 instrument (High Output Flow Cell, 75 bp, paired end).