GSM7732454: Cd248_KO_2; Mus musculus; RNA-Seq
Total RNA was extracted using Trizol reagent (thermofisher, 15596018) following the manufacturer's procedure. After total RNA was extracted, mRNA was purified from total RNA (5ug) using Dynabeads Oligo (dT) (Thermo Fisher, CA, USA) with two rounds of purification. A cDNA library constructed by technology from the pooled RNA from fibrotic kidney samples of mice was sequenced run with Illumina NovaseqTM 6000 sequence platform.