GSM7030156: npd07; Saccharomyces cerevisiae; OTHER
Genomic DNA was purified by Phenol:Chloroform:Isoamylalcohol approach. Library preparation of isolated DNA was performed with the SQK-LSK109 ligation sequencing kit (Oxford Nanopore Technologies) according the manufacturer's protocol. For multiplexing up to 24 samples, native barcode expansion kits (EXP-NBD104/114) were used. Libraries were loaded onto MinION R9.4.1 flow cells (Oxford Nanopore Technologies) and run for 72 hrs using MinKNOW software (version 20.06.4) with disabled basecalling, barcoding and alignment. Output was saved in FAST5 format.