RNA-seq?of?V.?vulnificus?MO6-24/O?:?Biofilm_10h_2nd
Total cellular RNA was isolated from the cells harvested at each time point using an miRNeasy kit (Qiagen). Ribosomal RNAs were removed from total RNA by using Ribo-Zero rRNA Removal kit (Epicentre, Madison, WI). cDNA libraries were generated using TruSeq Stranded mRNA sample prep kit (Illumina, San Diego, CA) following manufacturer's instruction.