mtDNA-Seq of Homo sapiens: pediatric whole blood sample
Approximately 400 l of blood in EDTA tubes were processed using a MagPurix robotic system, resulting in 100 l of genomic DNA at a concentration of 30-100 ng/l as measured by NanoDrop. Mitochondrial DNA was sequenced with IonTorrent S5 using the Ion AmpliSeq kit and aligned to the human mitochondrial reference genome (rCRS/NC_012920.1) with BWA mem (v.0.7.12).