RNA-Seq from leaves of tomato with stress watering and HB treatment
The mRNA was isolated with magnetic beads of oligos d(T)25 (NEBNext Poly(A) mRNA Magnetic Isolation Module) and subsequently, was randomly fragmented. cDNA synthesis was performed using random hexamers and Illumina buffer, employing the kit NEB Next Ultra RNA Library Prep Kit. Illumina's Sequencing Technology by Synthesis (SBS)- was used to sequence the libraries. The strategy is paired end 150bp (PE150).