GSM7597564: Mosaic Tumor Replicate 3 ZipSeq; Mus musculus; RNA-Seq
Tumor sections wwere digested in a Collagenase I (100U/mL) and IV (400U/mL) blend in RPMI 1640 for 30 minutes at room temperature. CD45+ cells were sorted using a FACSAria for encapsulation For gene expression (GEX) libraries, we followed the manufacturer's instructions for 10x 3' v3 library construction.