GSM7789621: Multiplexed_GEX: [HW1, CSF] + [HW1, TME (GM)] + [MN1, CSF] + [MN1, TME (GM)]; Homo sapiens; RNA-Seq
Single-cell suspensions of sub-confluent GBM cells, cultured in parallel in either GM or CSF were added to weels of an 8-channel microfluidic chip and transferred to a Chromium Controller. Six cell lines (468, 469, 497, BAH1, HW1 and MN1) were labelled with lipid-conjugated Cell Multiplexing Oligonucleotides (10X Genomics) according to manufacturer's recommendations for cell pooling prior to loading onto a 10X Genomics chip. Single-cell pooled suspensions were loaded on a GemCode Single Cell Instrument (10X Genomics) to generate single-cell gel beads in emulsion (GEM). scRNA-seq libraries were prepared from GEMs using a 3' CellPlex Kit Set A (10X Genomics) and a Chromium i7 Multiplex kit (10X Genomics) according to the manufacturer's instructions.