GSM7720994: Trophozoites, RNAseq_MorcKD_Mel1; Plasmodium falciparum; RNA-Seq
Total RNA was extracted following the manufacturer's protocol, and an RNA cleanup kit (Qiagen) was used to achieve high-purity RNA. The isolated RNA was quantified using a NanoDrop ND-1000 UV/Vis spectrophotometer, and RNA quality was determined using an RNA ScreenTape System (Agilent 2200 TapeStation). Total RNA (10000 ng) from each sample was stabilized in RNAStable (Biomatrica) and sent to the Micromon Genomics facility at Monash University for next-generation sequencing. RNA samples were prepared using the MGITech RNA Directional Library Prep Kit V2 (Item No. 1000006385), as per the manufacturer's instructions, with the following parameters: input RNA: 50 ng, fragmentation: target of 200 bp-400 bp, 87 °C for 6 mins, adapter clean-up: 200 bp-400 bp and library amplification cycles: 16. Libraries were assessed for quantity using the Invitrogen Qubit and dsDNA HS chemistry (Item No. Q33230) and quality/quality using the Agilent Fragment Analyser 5200 with the HS NGS Fragment Kit (Item No. DNF-473-0500). The libraries were pooled in equimolar concentrations and sequenced using an MGITech DNBSEQ-G400RS sequencing instrument with High-Throughput Sequencing Set (FCL PE100, Item No. 1000016950) chemistry.