Genome sequencing of Galbibacter sp.
Cell pellets were resuspended in ATL buffer (Qiagen) containing RNAse A. ZR BashingBead Lysis Tubes (Zymo Research) were used for cell disruption. DNA was isolated using QIAmp 96 DNA QIAcube HT kits with addition of proteinase K (Qiagen). Libraries for short-read sequencing were prepared using the Illumina DNA Prep Tagmentation kit with 500 ng DNA input and 5 cycles indexing PCR. Library quality was evaluated (Agilent 2100 Bioanalyzer) and sequenced at an Illumina NovaSeq using a NovaSeq 6000 SP v1 sequencing kit with 2x150 bp read length and a depth of 4.05.0 Mio reads per sample. The sequencing was demultiplexed (Illumina bcl2fastq, v2.19.0.316), quality-checked (Fastq, v0.20.1) and visualized (MultiQC, v1.7). Paired-end reads were quality-filtered (Fastp (5) v0.20.1), assembled (Unicycler (6) v0.4.8), quality-checked (CheckM (7) v1.0.18) and genomes were annotated using Bakta (8) (v1.5.1).