GSM7597523: AAA patient 4 (techn. Rep. 2); Homo sapiens; RNA-Seq
After enzymatic digestion, tissue dissociation was performed by using the GentleMACS Dissociator according to the manufacturer's instructions. The cell suspension was strained (70 µm, 40 µm) and Dead Cell Removal (Miltenyi Biotech, 130-090-101) using MS Columns (Miltenyi Biotech, 130-042-201) was performed. Cells were resuspended in PBS + 0,04% BSA. Cells were loaded into a 10x Genomics microfluidics Chip G and encapsulated with barcoded oligo-dT-containing gel beads by utilizing the 10x Genomics Chromium Controller. Gel Beads-in-emulsion (GEM) clean-up, cDNA Amplification and 3'Gene Expression Dual Index Library Construction was performed according to the manufacturer's instructions (CG000315 Rev C). Sequencing was performed by taking advantage of an Illumina NovaSeq 6000 Sequencing system.