GSM8112583: Ec78_RepreRad_rep3; Escherichia coli; RNA-Seq
After induction, bacteria were grown to an OD600of 1. Total RNA was extracted from cells using a miRNA isolation kit (QIAGEN, Catalog number #217004) according to the manufacturer's recommendation. RNA sequencing was performed utilizing Transcriptome Sequencing of the Macrogen Japan NGS service on the Novaseq6000 platform. The TruSeq Stranded Total RNA Library kit (for bacteria) (Illumina, San Diego, USA) was utilized subsequent to the removal of rRNA from the sample. The read length was 100 base pairs, and the sequencing run scale was set at 4 gigabytes per sample.