RNA-seq of human: HSPC
RNA-seq was performed based on SMART-seq2 method. In brief, two hundred cells were lysed, and reverse transcription was conducted, then 11 cycles of PCR were used to amplify transcriptome library. Quality of whole transcriptome libraries was validated using a D5000 DNA Chip run on a Bioanalyzer 4150 system (Agilent), followed by library preparation using the TruePrep DNA Library Prep Kit (Vazyme, Cat. # TD503) and custom index primers according to the manufacturers instructions. Two biological replicates per condition were sequenced in a Novaseq 6000 (S4, PE150, Illumina) by Novogene (Beijing).