GSM7911917: lung epithelial cell, KP infected, 48h, rep 3; Mus musculus; RNA-Seq
Lungs were perfused with 10 ml PBS, followed by adminitration of collagenase and DNase. Single cell suspensions were prepared for flow cytometry. Single cells were incubated with biotynilated Ly6G, CD11c, CD11b, CD31 and Siglec F for 30 minutes on ice at 4 ℃ in the dark and passed through LS colum for negative selection which was stained for CD45, CD31 and Epcam at 4oC for 20 min. The negative selection fraction (CD45-CD31-Epcam+) was sorted by FACSAriaTM (BD Bioscience).