No stim control for whole genome PSS of the nuclear periphery in wells of IMR90 cells
Fixed wells of IMR90 cultured cells underwent a gentile histone denaturing treatment (0.1N HCl for 5 minutes at room temperature), and were tagmented in situ using Tn5 Transposase loaded with blocked adapters. There was no targeted illumination for the no stim control sample. Uncaged fragments were amplified and sequenced on an Illumina platform.