GSM7989252: KPC B; Mus musculus; RNA-Seq
Cells were  homogenized in 1mL of Trizol and RNA extracted as per manufacters protocol Libraries were prepared by MUSC Translational Core using the New England Biolabs NEBNext Poly(A) mRNA Magnetic Isolation Module (Cat# 7490L) and Ultra II Directional RNA Library Prep Kit from Illumina (Cat# 7760L). Dual indexed libraries were pooled and sequenced at VANTAGE (Vanderbilt University Medical Center) on an Illumina NovaSeq 6000 to a depth of approximately 25 million paired-end 150 bp reads per library.