Bacterial 16S rDNA sequencing of OSCC sample
DNA was extracted from all samples using DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany). The concentration and purity of the isolated DNA were detected using 1% agarose gels electrophoresis. DNA was diluted to 1ng/l using sterile water. Genome DNA from each sample was used as an amplification template. PCR targeting of the V3-V4 region of the 178S rDNA hypervariable region was conducted