GSM7851423: MO-LAS-B cells, Paclitaxel, rep2; Homo sapiens; RNA-Seq
Total RNA was extracted using PureLink™ RNA Mini Kit (Thermo Fisher Scientific) according to the manufacturer's instructions. The total RNA was treated with RQ1 RNase-Free DNase (Promega, #M6101) at 37°C for 40 minutes and purified by phenol/chloroform extraction and ethanol precipitation. The 3'mRNA-seq library was prepared using the QIAseq UPX 3' Transcriptome Kit from QIAGEN, following the manufacturer's instructions.