NextSeq 500 paired end sequencing
Meyer and Kircher's (https://doi.org/10.1101/pdb.prot5448) library preparation protocol with the addition of Rohland et al.'s (https://doi.org/10.1098/rstb.2013.0624) partial UDG treatment and ligation of unique 7-mer 5' and 3' barcoded adapters during the ligation step (https://doi.org/10.1101/gr.128124.111)