GSM7300035: LNCaP_clone2, H3K27ac; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a E220 focused-ultrasonicator (Covaris). ChIP-seq libraries were prepared using the ThruPLEX DNA-seq Kit (Takara). 10 ng of DNA was used as starting material for input and IP samples. Libraries were amplified using 10 cycles on the thermocycler. Post amplification libraries were size selected at 200-500bp in length using Agencourt AMPure XP beads (Beckman Coulter). Library fragment size distributions were analyzed using 2100 Bioanalyzer High Sensitivity DNA Kit (Agilent). Diagenode Cat# C15410196 H3K27ac antibody was used.