GSM8195097: MEFs Untreated-1; Mus musculus; RNA-Seq
Total RNA was extracted using Trizol according to the manufacturer's instructions followed by isopropanol precipitation. Total RNAs were further purified using Qiagen's miRNeasy micro kit (217084, Qiagen) plus Qiagen's DNase set (79254, Qiagen) for on-column DNase digestion option. Sequencing library preparation and RNA-seq were conducted by Novogene. mRNA was purified using PolyA+ selection.