Isolation and characterization of lytic bacteriophages infecting Pectobacterium atrosepticum
the DNA sample is randomly interrupted and the DNA fragment of the required length is generated, the sticky end formed by the interruption is repaired to a flat end, and then the base "A" is added through the 3' end, so that the DNA fragment can be connected with the 3' special connector with the "T" base; Finally, the DNA fragments with adapters at both ends are amplified using PCR to complete the construction of the entire library. Constructed qualified libraries for cluster preparation and in-machine sequencing.