Whole genome sequencing (PacBio HiFi) of Biomphalaria pfeifferi: Whole Snail
High molecular weight (HMW) DNA extraction was performed from one B. pfeifferi using the Qiagen MagAttract HMW DNA Kit. DNA (10 g) was used in PacBios HiFi library preparation protocol (Procedure & Checklist - Preparing SMRTbell Libraries for HiFi Long Read Sequencing on Sequel and Sequel II Systems, Version 02 (May 2019)). The prepared DNA sample was loaded in a PacBio Sequel II  SMRT Cell instrument for whole genome sequencing. HiFi reads centered at 15-20 kb were obtained after 30 hours sequencing.