GSM7062267: OE-MAGI2-AS3_rep2; Homo sapiens; RNA-Seq
The total RNA was extracted according to the instruction manual of the TRlzol Reagent (Life technologies, California, USA).Purity, concentration and integrity of RNA sample were examined by NanoDrop, Qubit 2.0, Agilent 2100, etc. Only RNA with good quality could move on to following procedures. Sequencing libraries were generated using Hieff NGS Ultima Dual-mode mRNA Library Prep Kit for Illumina (Yeasen Biotechnology (Shanghai) Co., Ltd.) following manufacturer's recommendations and index codes were  added to attribute sequences to each sample.