GSM7850697: ce_smallRNA_mir235seed_TC_mut_18h; Caenorhabditis elegans; ncRNA-Seq
We harvested worms from 18h to 30h in hourly interval. Worms were collected from plates and washed 3 times and resuspended in Tri Reagent (MRC, TR118) and RNA was extracted using  Direct-zol™ RNA MicroPrep kit (Zymo Research; R2062) as per manufacturere's instructions. Total RNA was treated with DNAse on column Sequencing libraries were generated using CleanTag Small RNA Library Preparation Kit according to the manufacturer's instructions. The library pool was size selected on a gel (140-160 bp) to enrich for the small RNA fraction. Subsequently, library was sequenced on Illumina Next Seq500, as 51 cycles single-end reads.