GSM7816293: taPVAT_rep1; Rattus norvegicus; RNA-Seq
Nuclei from frozen minced aortic perivascular adipose tissue or subscapular brown adipose tissue (~ 50 mg) were isolated with the Chromium Nuclei Isolation Kit (10X Genomics) according to manufacturer's recommended protocol (CG000505, RevA), with a final resuspension in 50 µL Wash and Resuspension Buffer. The 10X Chromium Next GEM Single Cell 3' Reagent Kit v 3.1 (Dual Index) was used with a targeted cell recovery of 10,000 according to manufacturer's recommended protocol (CG000315, Rev D).