GSM7988659: CDX2-Het, replicate 2, snATACseq; Homo sapiens; ATAC-seq
For each sample within the batch, all wells of a 24-well plate that each contained 12 micropatterned colonies were pooled, yielding a cell suspension comprising approximately 288 colonies per sample.  Nuclei were isolated following the manufacturer's instructions (CG000365_DemonstratedProtocol_NucleiIsolation_ATAC_GEX_Sequencing_RevC.pdf). Nuclei were isolated and ~10,000-19,000 nuclei/sample were transposed and loaded onto a 10x Chromium Chip J to generate gel bead-in emulsions (GEMs) following the 10x Chromium Next GEM Single Cell Multiome ATAC and Gene Expression Kit (10x Genomics, CG000338). Multiomic scRNA-seq and scATAC-seq