GSM8409575: ΔhapR, LB, rep 3; Vibrio cholerae C6706; RNA-Seq
RNA was extracted and purified using Zymo Research "Direct-zol RNA Miniprep Plus Kit". 500ng of RNA was used for the construction of sequencing libraries RNA libraries for RNA-Seq were prepped and prepared using the RIboPOOLs kit from siTOOLs and a modified version of the RNAtag-Seq protocol to generate illuminastrand specific cDNA libraries.