GSM7850822: CHC20, tumor; Homo sapiens; OTHER
OCT-embedded tissue blocks were sectioned at 10 μm thickness using Cryostar NX70 (Leica) and tumoral tissues containing RNA with a RIN ≥ 6.8 were included in the Visium experiments. One section per patient was cut at 10 μm thickness and placed on Visium slide capture areas (10x Genomics). Slides were then processed following the 10x Genomics Visium Spatial protocol according to the manufacturer recommendations. The slides containing tissues were methanol-fixed at -20°C and processed for H&E staining and imaging. Spatial transcriptome sequencing libraries were prepared with 10xGenomics Visium Spatail Gene Expression Slides & Reagent kit according to manufacturer's construction