Hi-C Seq of mus musculus
Sample cells are fixed with formaldehyde for the cross-linking of DNA and proteins, facilitating the study of protein-protein interactions. Once the cross-linking is completed, the cells are lysed, and samples are taken for quality testing. After passing the quality test, the samples undergo the "Hi-C fragment" preparation process. This involves digesting the chromatin with a restriction enzyme, followed by testing the effectiveness of the enzyme digestion. Next, the samples are subjected to biotin labeling, blunt-end ligation, and DNA purification to prepare Hi-C samples. Simultaneously, samples are taken for DNA quality testing. Once the quality test is passed, the samples proceed to the standard library construction process. This process includes removing biotin from the Hi-C fragments, fragmenting the DNA through sonication, repairing the DNA ends, adding A bases, and ligating sequencing adapters to form adapter-ligated products. PCR condition screening and amplification are then performed to obtain library products. Samples of the library amplification products are taken for Hi-C junction quality control testing. Once the quality control is passed, the library preparation is considered complete. The constructed library is then sequenced using the MGI-2000 sequencer, employing a sequencing strategy of PE150.