DSM_41527
As provided by sequencing and bioinformatics supplier (microbesng.com; MicrobesNG, Units 1-2 First Floor, The BioHub, Birmingham Research Park, 97 Vincent Drive, Birmingham, B15 2SQ, UK): DNA extracted by MicrobesNG, or sent directly by the customer is then quantied with the Quant-iT dsDNA HS kit (ThermoFisher Scientic) assay in an Eppendorf AF2200 plate reader (Eppendorf UK Ltd, United Kingdom) and diluted as appropriate. Illumina Sequencing (SGS and EGS) Genomic DNA libraries are prepared using the Nextera XT Library Prep Kit (Illumina, San Diego, USA) following the manufacturers protocol with the following modications: input DNA is increased 2-fold, and PCR elongation time is increased to 45 seconds. DNA quantication and library preparation are carried out on a Hamilton Microlab STAR automated liquid handling system (Hamilton Bonaduz AG, Switzerland). Libraries are sequenced on an lllumina NovaSeq 6000 (Illumina, San Diego, USA) using a 250 bp paired end protocol. Reads are adapter trimmed using Trimmomatic version 0.30 with a sliding window quality cutoff of Q15.