GSM8150604: WT_Rep2; Trypanosoma brucei; RNA-Seq
Trypanosomes were isolated from the blood of infected mice at 5 dpi (peak of higher parasitemia). Total RNA was isolated from parasites of the first peak of parasitemia, obtained by differential centrifugation. Extraction and sequencing of at least two independent biological samples for each sample proceeded as described by Saura et al. (PMID: 31693280). Libraries from mRNA were prepared using the Illumina stranded mRNA Prep Ligation kit (Illumina, CA, USA) according to the manufacturer's protocol. Quality (RNA integrity) and size distribution of indexed mRNA libraries were validated through Bioanalyzer High Sensitivity DNA assay in a Bioanalyzer 2100. Concentration was measured on the Qubit fluorometer. Final libraries were pooled in an equimolecular manner and then diluted and denatured as recommended