Nano-DMS-MaP of HIV-1 5'-UTR: unspliced mRNA cell 0mM DMS rep2
transfection of plasmid expressing HIV-1 NL4-3 del Gp, 24 h p.tfx. DMS probing, RNA extraction of cells, RT with RT-US, PCR with PCR-HIV_Fw and PCR-US_Rv, and sequencing with native barcoding kit Nanopore SQK-NBD112.96