Amplicon sequencing
DNA extraction, sample preparation including amplification of V1-3 region of 16S rRNA gene using the 27F (AGAGTTTGATCCTGGCTCAG) (Lane, 1991) and 534R 194 (ATTACCGCGGCTGCTGG) (Muyzer et al., 1993) primers, and amplicon sequencing were conducted as described by Dottorini et al., (2021)