GSM7597456: Worm, 0.25mg/mL Cyclo (Pro-Tyr), rep1; Caenorhabditis elegans; RNA-Seq
RNA from synchronized C. elegans N2 was extracted as previously described with slight modifications. Briefly, C. elegans N2 was incubated on M9 buffer supplemented with 0.25 mg mL-1 of Cyclo (Pro-Tyr), and DMSO as control, for 24 h at 19ºC with gentle shaking. After that, worms were centrifuged and supernatant was discarded, worm pellet was resuspended in TRI-Reagent (Simga-Aldrich) with 0.1 mm glass beads and worms were lysate in TissueLyser II (Qiagen) at 30 cycles sec-1 for 20 min. After worm lysis, RNA was extracted using NucleoSpin RNA Plant and Fungi kit (Macherey-Nagel), according to the manufacturer's instructions. For RNA sequencing analysis, 200-500 bp single-end read libraries were prepared using a TruSeq stranded total RNA kit (Illumina). The libraries were sequenced using a NextSeq550 instrument (Illumina).