GSM8324649: ASeq2-19D,rep2; Homo sapiens; ATAC-seq
After ATAC-Seq, the transposed DNA samples were purified by Qiagen minElute column. ATAC-seq libraries are prepared based on Howard Chang protocol from Nature Methods, 2017. qPCR was used to determine the optimal PCR cycles to prevent over-amplification of transposed DNA. Purified transposed DNA is subjected to total of 11 cycles of qPCR amplification to make ATAC-Seq libraries.