GSM7062708: mutation-2; Homo sapiens; RNA-Seq
Total RNA was isolated from cultured cells using TRIzol reagent (ThermoFisher Scientific, 15596018) based on the manufacturer's protocol. 2 μg RNA was reverse transcribed to cDNA using the PrimeScript RT reagent with a gDNA eraser Kit (Takara, no. RRO47B). RNA libraries were prepared for sequencing using standard Illumina protocols