GSM7789600: Control virus transduction, VIP treatment, rep 1; Mus musculus; RNA-Seq
Cortical astrocytes were washed with 1X PBS and lysed directly using 1 mL of TRIZOL. From TRIZOL mix, the total RNA was column purified using the Qiagen RNeasy Kit. Strand-specific cDNA libraries (~250-300bp) were prepared for RNA-seq by NovogeneAIT Genomics (Singapore) and 20M 150bp paired-end reads per sample were generated by sequencing on the Illumina Novaseq 6000 platform.