GSM7988848: M3_rep3; Rattus norvegicus; RNA-Seq
Mammary glands were collected freshly and digested in digestion medium containing 2 mg/mL collagenase type IV, 2 mg/mL hyaluronidase and 2 mg/mL BSA in DMEM/F12 medium, at 37 ºC for 1 to 2 hours, then viable single cells were submitted to Translational Immunogenomics Lab core in Dana-Farber cancer institute for library construction. The scRNA-seq libraries were prepared according to the Chromium Next GEM Single Cell 5' HT v2 protocol (10x Genomics). Quality control for amplified cDNA libraries and final sequencing libraries were performed using Bioanalyzer High Sensitivity DNA Kit (Agilent). The pooled libraries were sequenced on Illumina NovaSeq S4 platform.