GSM7029328: ATAC-Seq WT; Mus musculus; ATAC-seq
Cell suspensions were brought to the UM Epigenomics Core for ATAC-Seq library preparation. We used the Omni-ATAC-Seq protocol (Corces et al., 2017).  The final libraries were cleaned using Qiagen MinElute columns and AMPure XP beads before quantitation with the Qubit HS dsDNA kit, and quality assessment on a TapeStation HS D1000 kit. The libraries were pooled and quantitated by qPCR using KAPA's Illumina Library quantitation kit before PE-150 sequencing on a shared flowcell on the NovaSeq6000 at the Advanced Genomics Core