Plasmid sequencing of miniprepped, restriction digest linearized (SceI) : synthetic plsamid pool of barcodes and ORFs
Plasmids were miniprepped from E. coli using Qiagen miniprep kit, digested using I-SceI, observed to be linear and intact, then prepared for sequencing using ONT Nanopore ligation kit. sequencing/sequencing_MIN112_DNA_SQK-Q20EA:FLO-MIN112:SQK-NBD112-24 This time I used nick-repaired or treated with due respect, and many fewer reads appear truncated.