RNA-seq soil metagenome
Bacterial 16S rRNA genes were PCR-amplified with dual-barcoded primers targeting the V4 region (515F 5-GTGCCAGCMGCCGCGGTAA-3, and 806R 5-GGACTACHVGGGTWTCTAAT-3), as per the protocol of Kozich et al. (2013). Amplicons were sequenced with an Illumina MiSeq using the 300-bp paired-end kit (v.3) (Illumina Inc., San Diego, CA).