GSM7815522: MeDIP_Normal Sample 2; Homo sapiens; MeDIP-Seq
Genomic DNA was extracted using the proteinase K & phenol-chloroform method. The tissue samples (10-30mg) were homogenized in 2 ml of SET buffer followed by centrifugation. The supernatant was completely removed & the pellet was lysed with 1 ml lysis buffer and was subjected to Proteinase K digestion at 37 °C for 12h. The mixture was afterward treated with phenol: chloroform: isoamyl alcohol (25:24:1) and centrifuged at 25 °C. Next, DNA was precipitated with 1/30 volume of 3 M sodium acetate (pH 5.0) and two volumes of chilled absolute ethanol and washed with 70% ethanol. Finally, the DNA pellet was re-suspended in TE buffer and stored at 4°C for further use. For MeDIP sequencing, sequencing librabries were prepared using MagMeDIP kit following manufacturer's protocol.