16S rRNA gene analysis of Bacterial DNA obtained from fecal samples: patient 31
We performed amplicon sequencing of bacterial 16S rRNA gene on human stool samples from 32 control individuals, 42  individuals diagnosed with intestinal polyps and 3 individuals diagnosed with CRC. The procedure was as follows: Thawing of frozen human fecal samples, homogeneization, centrifugation and bacterial DNA extraction from fecal pellets using Protocol Q followed by 16S rRNA gene analysis