Bisulfite-Seq of Nymphaea thermarm: mature endosperms from individual rp0039
Mutiple endosperms from seeds from a single fruit were pooled and DNA extracted using QIAamp micro kit. Libraries were prepared using a Zymo Pico Methyl-Seq Library Prep Kit, as per manufacturer's instructions, starting with section 2 using previously bisulfite-converted samples as input. Fragment size selection (>200 bp) were selected with a PippenHT. Libraries were pooled with equlimolar concentrations and were sequenced on three lanes of an Illumina Hiseq at the Whitehead Genome Core.