16s seq of Mus musculus: adult male fecal
The 16S rDNAtarget region of the ribosomal RNA gene were amplified by PCR 95 C for 5 min,followed by 30 cycles at 95 C for 1 min , 60 C for 1 min , and 72 C for 1 min and a final extensionat 72 C for 7 min using primers listed in the table. PCR reactions were performed in triplicate50 L mixture containing 10 L of 5  Q5 Reaction Buffer , 10 L of 5  Q5 High GCEnhancer 1.5 L of 2.5 mM dNTPs, 1.5 L of each primer ( 10 M), 0.2 L of Q5 High FidelityDNA Polymerase , and 50 ng of temp late DNA. Related PCR reagent s were from New EnglandBiolabs USAIllumina