ONT WGS of Elysia crispata: whole slug
Genomic DNA was extracted from E. crispata whole bodies of wild adults starved for a minimum of 2 weeks. A CTAB or DNAzol protocol was used to generate high molecular weight DNA. At least 1.9 g of high molecular weight genomic DNA was used as input for ONT LSK-109 library ligation kits and sequenced on an R9 MinION flow cell. Base calling of ONT reads was performed with Guppy v6.1.2 (Oxford Nanopore Technologies, Oxford, UK).