Illumina WGS of Elysia crispata: egg
Genomic DNA was extracted from E. crispata eggs reared in petri dishes. Samples were pulverized in liquid nitrogen using a Tissue Lyser II (Qiagen, Hilden, Germany). DNA was extracted using one of two methods, a CTAB-based protocol for samples with high mucopolysaccharide content (dx.doi.org/10.17504/protocols.io.kxygxp81zl8j/v1) modified from (Winnepenninckx et al. 1993) or a DNAzol Reagent (Invitrogen, Waltham, MA) protocol (dx.doi.org/10.17504/protocols.io.n92ldprrnl5b/v1). Quantity and quality of DNA for each sample was assessed using a Qubit 4 fluorometer (Invitrogen, Waltham, MA) and a TapeStation 4150 (Agilent, Santa Clara, CA). No difference in quality between the CTAB and DNAzol-based protocols were observed, and the samples were pooled prior to Illumina sequencing. The sequencing library was constructed using an NEBNext DNA library prep kit (New England Biolabs) and sequenced using an Illumina NovaSeq 6000 by Novogene Corporation Inc. (Sacramento, CA).