Illumina NovaSeq 6000 paired end sequencing; Primed to resolve: A single cell atlas of the shoulder capsule reveals a cellular basis for resolving inflammatory fibrosis
Primed to resolve: A single cell atlas of the shoulder capsule reveals a cellular basis for resolving inflammatory fibrosis
Samples were collected from the rotator interval of the shoulder joint capsule. Cell suspension was sorted using SONY SH800 cell sorter. 20,000 - 30,000 cells were loaded onto a Next GEM Chip to create single cell emulsion on the 10x Chromium Controller. Single cell gene expression libraries were prepared using the 10x Chromium Next GEM Single Cell 3' Reagent Kits v3.1.