Illumina HiSeq 4000 paired end sequencing; Early time-response in estrogen regulated transcriptome in Atlantic cod (Gadus morhua) precision-cut liver slices culture exposed to ethynylestradiol
Early time-response in estrogen regulated transcriptome in Atlantic cod (Gadus morhua) precision-cut liver slices culture exposed to ethynylestradiol
A total of 5 males juvenile cod (G. morhua) were used for PCLS (n = 5/group). PCLS preparation and chemical exposure in the culture was performed as previously described (Yadetie et al., 2021 et al., 2021). Atlantic cod liver slices were cultured in 24-well plates in the growth medium containing DMSO (vehicle control) or EE2 (10 nM). For time response study, the slices were collected at 12, 24 and 48 h after exposure to DMSO control or  10nM EE2 in the culture. The slices were snap frozen in liquid nitrogen and stored at -80 Â°C for RNA extraction. RNA extraction was performed from a total of 30 samples comprising 6 groups (three time points with 10 nM EE2 and time-matched control groups, n = 5/group). RNA extraction was performed using TRIreagent following the manufacturers protocols (Sigma-Aldrich Oslo, Norway). Poly(A) pluss RNA sequenced on Illumina HiSeq 4000 system (50 million 2x75 bp paired-end option)at Genomic Core Facility, University of Bergen, Norway