WGS of Otocolobus manul: adult female blood
A total amount of 0.2 g DNA per sample was used as input material for the DNA library preparations. The genomic DNA sample was randomly fragmented by sonication to a size of 350 bp. Then DNA fragments were endpolished, A-tailed, and ligated with the full-length adapter for Illumina sequencing. The fragments with adapters were size selected, PCR amplified, and purified by AMPure XP system. Subsequently, library quality was assessed on the Agilent 5400 system and quantified by QPCR (1.5 nM). The qualified libraries were pooled and sequenced on Illumina NovaSeq X Plus platform with PE150 chemistry.