Penticton biomass incubation with 13C acetate identified as replicate 3, and bouyant density gradient fraction 12
Amplicon sequencing on the MiSeq v2 (2x300bp) kit with libraries prepared using a PCR with primers 515F/926R. Prior to 16S rRNA gene amplicon sequencing, the fractionated DNA was pre-amplified using non-barcoded 515F/926R primers and Q5 Hot Start High-Fidelity 2X Master Mix for 18 PCR cycles