NextSeq 500 paired end sequencing; Transcriptomic profiling of keratinocytes isolated from patients with rare inflammatory skin disorders
Transcriptomic profiling of keratinocytes isolated from patients with rare inflammatory skin disorders
Primary keratinocytes were isolated from patients with inflammatory skin disease in addition to controls which were subsequently immortalised (Lenti-HPV-16 E6/E7) RNA was extracted using the RNAeasy plus mini kit (Qiagen, Germany), using RLT buffer to lyse cells, 70-90% ethanol to precipitate the nucleic acids and nuclease free water to resuspend the RNA.  cDNA libraries were prepared using the KAPA mRNA HyperPrep Kit (Roche)