GSM7851417: AC18-R3; Olea europaea var. sylvestris; RNA-Seq
Total RNA was isolated and purified from three independent biological replicates using an automated extraction system Maxwell® 16 Lev Plant RSC Instrument (manufactured by Promega Corporation, Wisconsin, USA) using a Maxwell® RSC RNA FFPE kit (AS1440, Promega, Wisconsin, USA). The process was conducted according to the manufacturer's instructions. Illumina's TruSeq Stranded mRNA Library Prep Kit was used to prepare the libraries following the manufacturer's instructions. Briefly, each sample was enriched in mRNA by selection 3' poly(A) tails using poly(T) magnetic beads. Captured mRNAs were then converted into cDNA, and sequencing adaptors were added to their ends to make the molecules ready for sequencing. Samples were dual-indexed for post-sequencing demultiplexing. The fragment size distribution and concentration of the libraries were checked in an Agilent 2100 Bioanalyzer using the Agilent DNA 1000 kit. These were sequenced into a HiSeq-4000 Illumina platform as 2x100b paired-end sequences.