GSM7733849: Colon muscularis propria, SPF, 2; Mus musculus; RNA-Seq
RNeasy Mini Kit from Qiagen (cat # 74104) was used to extract RNA following the manufacturer instructions. For the lysis and homogenation step, Qiagen PowerBead Tubes with Garnet 0.70mm (cat #13123-50) were used with a bead beater for 1 minute at 3450 oscillations/min (GlenMills, Beadbeater-16). RNA quality and quantity was assessed using the Agilent bio-analyzer. Strand-specific RNA-SEQ libraries were prepared using a TruSEQ based mRNA-SEQ library protocol (Illumina provided). Library quality and quantity was assessed using the Agilent bio-analyzer and libraries were sequenced using an Illumina NovaSEQ6000 (Illumina provided reagents and protocols).