GSM7029372: digested periodontal ligament single-cell suspensions; Homo sapiens; RNA-Seq
In brief, dental pulp tissues and periodontal ligament were removed from teeth, minced, and digested in a solution of 3mg/mL collagenase type I and 4mg/mL dispase (Sigma-Aldrich, St.Louis, MO) for 30–60 min at 37°C. cDNA synthesis was performed using the BD Rhapsody Express Single-Cell Analysis System, and gene expression libraries were prepared using Whole Transcriptome Analysis Amplification Kit (all from BD Biosciences).Whole Transcriptome Analysis Amplification Kit (all from BD Biosciences).