RNA-Seq of mat: control2
mRNA was extracted with RNA PowerBiofilm extraction kit, following the instructions of the manufacturer, in a RNAase free laboratory at Parque Cientfico de la Comunidad de Madrid. DNAase was employed to remove the remaining DNA. Then, reverse transcriptase was used to produce cDNA. The V3-V4 region from the rRNA 16S gen was amplified from the cDNA using universal 16S primers 341 F (5-CCTAYGGGRBGCASCAG-3) and 806 R (5-GGACTACNNGGGTATCTAAT-3)