GSM7989593: LNCaP_0m_rep2_ATACseq; Homo sapiens; ATAC-seq
DNA was purified using Qiagen MinElute (#28004), The nuclei pellets were resuspended in 50 μl of transposition buffer (25 μl of 2× TD buffer, 22.5 μl of distilled water, 2.5 μl of Illumina Tn5 transposase) and incubated at 37 °C for 30 min with shaking at 1000 r.p.m for fragmentation. Transposed DNA was purified using Qiagen MinElute (#28004), and the library was amplified up to the cycle number determined by 1/3rd maximal qPCR fluorescence.