GSM8062755: Ntg-PLX-467; Mus musculus; RNA-Seq
Following treatment, mouse microglia were isolated from the brain utilizing Adult Brain Dissociation Kit (Miltenyi Biotec, Auburn, CA) according to the manufacturer's instruction. Briefly, enzyme-mixed chopped brains were dissociated by gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec) for 30 minutes. The brain homogenates were filtered with 70 mm strainer, and incubated with CD11b magnetic beads (Miltenyi Biotec) at 4°C. After a 15-minute incubation, the beads were rinsed with MACS buffer (Miltenyi Biotec) and microglia were eluted from the brain by the pressure TruSeq Stranded mRNA LT Sample Prep Kit/TruSeq Stranded mRNA Sample Preparation Guide, Part #15031047 Rev. E