GSM8195251: week2_2; Mus musculus; RNA-Seq
C57BL/6J mice were implanted intracranially with 100,000 GL261-TVA/G cells into the right striatum. Seven or fourteen days after implantation, the tumor site was injected with EnvA-RABDG at a concentration of 10,000 infectious units per mL. Seven days after EnvA-RABDG injection, mice were sacrificed and CNS was isolated using methods identical to those of human GBM samples above, except that sorted mCherry+ cells underwent single cell droplet encapsulation using 3' feature barcode kits from 10X genomics. Library was constructed using 3' feature barcode kits from 10X genomics