DNA-Seq connect enhancer to barcode for INPUT_pPSint_v2_pTRP1_OP1_OP2
In order to connect barcodes to enhancers and libraries, the relevant region from the cloned libraries were sequenced with next generation sequencing (NGS). To avoid a constant start of R1 and R2 in the illumina run due to amplification using the constant sequence around the barcode and enhancer, primers were chosen to create variation in read start. Every forward or reverse primer used was a combination of 4 primers that each have a different, 0-3 bp of shift before the constant sequence. Primers P9-12 & P13-16 were used for pPSup_iGFP_v2 (35S.SynJ-OP1), P17-P20 & P21-24 used for pPSint_v2 (35S.SynJ-OP1) and pPSint_v2 (35S.SynJ-OP1+OP2),  P17-P20 & P25-28 used for pPSint_v2_rmBsaI (35S.SynJ), P13-16 & P29-32 used for pPSup_iGFP_v2_pTRP1 (pTRP1-OP1), P33-36 & P21-24 used for pPSint_v2_pTRP1 (pTRP1-OP1) and pPSint_v2_pTRP1 (pTRP1-OP1+OP2), and P33-36 & P25-28 used for pPSint_v2_pTRP1_rmBsaI (pTRP1).