GSM7850798: patient 18, tumor; Homo sapiens; RNA-Seq
15.000 single CD45+ panTCRαβ- CD19- innate immunity cells were loaded into a chip to form Gel Bead-in-Emulsion in the Chromium Controller. Single-cell libraries were generated using the Single Cell 3' reagent Kit v3.1 (10X Genomics) as per the manufacturer's protocol. cDNA was amplified by 12 PCR cycles and 12 cycles were also performed for library preparation (single index PCR). Libraries were pooled and sequenced on a NovaSeq 6000.