GSM7912308: WR3; Homo sapiens; RNA-Seq
Total RNA were extracted using Direct-zol™ RNA Microprep (Zymo Research) according to the manufacture instructions Total RNA was assessed for quality using an Agilent Tapestation 4200, and samples with an RNA Integrity Number (RIN) greater than 8.0 were used to generate RNA sequencing libraries using the TruSeq Stranded mRNA Sample Prep Kit with TruSeq Unique Dual Indexes (Illumina, San Diego, CA). Samples were processed following manufacturer's instructions, starting with 1 ug of RNA and modifying RNA shear time to five minutes.