GSM7911925: C_1; Ovis aries; RNA-Seq
Thirty-six 5-month-old Tan sheep with similar health statuses and weights were selected and randomly divided into four groups, with 9 sheep in each group, and reared in individual pens (4 m × 4 m). The four groups were as follows: control group (basal diet), SUC1 group (basal diet + 0.5% succinate), SUC2 group (basal diet + 1% succinate) and SUC3 group (basal diet + 2% succinate). The diets of each group were formulated according to the "Nutritional Requirements of Sheep" (Ministry of Agriculture of China, 2021) and the actual production of Tan sheep. The pretrial period was 10 days and the formal trial period was 60 days. During the experiment, all experimental sheep had free access to water and were fed twice a day (at 06:30 and 17:30).