GSM7681638: H23 treated with MRTX+TNO after 8 doublings, Replica 2; Homo sapiens; OTHER
Cells were lysed with Tris/SDS buffer supplemented with protease inhibitor, and DNA was precipitated with 40% isopropanol Region contatining sgRNA sequence was amplified with two sequenctial PCR and final product was gel purified--genomic library is recovered