16S amplicon-seq of UKKKH_TAU11_t0_mother_oral
DNA from oral swabs was isolated using the RNeasy Power Microbiome Kit (Qiagen, Germany). Seven of the 9 variable bacterial 16S rDNA regions (pool 1: V2, V4 and V8; pool 2: V3, V6/7 and V9) were amplified with the Ion 16S Metagenomics Kit (Thermo Fisher Scienctific, USA) utilizing two primer pools. Amplicons were pooled and cleaned using the NucleoMag NGS Clean-up (Macherey-Nagel, Germany), followed by library preparation with the Ion Plus Fragment Library Kit (Thermo Fisher Scienctific, USA).