16S rRNA sequencing of biofilms grown on oil-coated adsorbents
For amplicon sequencing, fragments covering the V3-V4 region of the 16S rRNA gene were amplified with (KAPA Biosystems) using the primer pair Bac341F/Bac805R. In a second PCR, the PCR products were supplied with Illumina adaptor overhang sequences. PCR purification, indexing with the Nextera XT Index Kit (Illumina), library quantification, pooling, and sequencing.