RNA-Seq of Bos indicus: Mature Y-sorted spermatozoa
Sex sorted (X/Y) and unsorted spermatozoa of progeny tested/pedigreed bulls (Bos indicus, Sahiwal breed) of high genetic merit were used for isolation of quality spermatozoal RNA using TRIzol reagent (Qiagen). NEBNext Ultra II Directional RNA library Prep Kit was used to prepare high quality libraries, and paired end sequencing reads of read length 151 bp were generated using Illumina HiSeq-X seqeuncing platform.