GSM7790767: HEK293T_non-target_VP64_H3K4me3_ChIP; Homo sapiens; ChIP-Seq
Transfected cells were cross-linked with 1% formaldehyde for 10 min and the cross-linking was neutralized with 125 mM glycine for 5 min at room temperature. Cell pellets were lysed in 1% lysis buffer. Sequencing libraries were prepared using TruSeq ChIP Library Prep Kit (Illumina)