GSM7431340: HDMBO3_A485_6H_bio_rep3; Homo sapiens; RNA-Seq
Cells were spun at 1200rpm x 5 minutes at 4°C and counted using trypan blue exclusion. 250,000 cells were resuspended in 1mL of TRIZOL, including relevant concentrations of ERCC spike in mix 1 (Thermo-Fisher), normalized to cell number. RNA was extracted using the TRIZOL extraction protocol, with DNAse 1 treatment (Thermo-Fisher Scientific), prior to resuspension in sterile, nuclease free water for library preparation. Total RNA was subjected to ribosomal RNA depletion prior to library preparation using the TruSeq Stranded Total Library Prep Gold Kit (Illumina).