GSM7432140: pancreatic cancer shNC1; Mus musculus; RNA-Seq
Single-cell library preparation was performed according to the manual of the Chromium Next GEM Single-cell 5' Reagent Kits v2 (Dual index). The cell suspensions were loaded on the Single-cell G Chip for gel bead-in-emulsion (GEM) generation and barcoding. cDNA library thus was generated with a droplet-based sequencing platform (10X Genomics). Next, reverse transcription, RT cleanup cDNA amplification, and quantification was performed to construct a 5' gene library. The libraries were sequenced on DNBSEQ-G400 (MGI Tech).