GSM8062469: RAW264.7 cells, Sync-label Enrich, Blank, rep1; Mus musculus; RNA-Seq
Cells grown in cell culture dishes at ~90% confluency were incubated with 50 nM photocatalyst in fresh medium at 37°C under 5% CO2 for 30 min, washed with PBS for three times, incubated with 50 µM QMPAB-2 in culture medium for 30 min and washed twice with PBS. After washing, cells were maintained in fresh medium at 37°C under 5% CO2 for 5 min and illuminated with blue LED (4 mW cm-2; λmax ∼460 nm) for 15 min at room temperature. Cells were placed about 5 cm from the light source. After illumination, cells were immediately collected using cell scraper and lysed with TRIzol reagent. Cellular total RNA purification was operated following the manufacture protocol by isopropanol precipitation.Total RNA was extracted using TRIzol reagent(Invitrogen). RNA libraries were prepared using SMARTer Stranded Total RNA-Seq Kit v2(Takara). Labeled RNA was captured using Dynabeads MyOne Streptavidin C1 (Invitrogen).