GSM7989564: SiNC_1; Rattus norvegicus; RNA-Seq
TRIzol (Ambion) solubilization and extraction were used to obtain total RNA. The total RNA was purified by RQ1 DNase (Promega) to remove residual DNA. The RNA quantity and quality were assessed by calculating the absorbance at 260nm/280nm (A260/A280) with Nanodrop One (Thermo). 150 nt paired-end sequencing was performed using Illumina NovaSeq 6000 system