RB-TnSeq of E. coli in the lake water, control
Whole-genome DNA was extracted from the cell pellet, and then the barcodes were PCR-amplified using the transposon-specific primers carrying Illumina technical sequences. The PCR-products (200 bp) were then pooled together, purified with AMPure beads (0.9x) and sequenced. The library quality was confirmed by Qubit HS kit and Bioanalyzer.