GSM6798295: c3Seq_22NC_rep3; Homo sapiens; OTHER
Total RNA was isolated using TRIzol followed by two consecutive phenol-chloroform extractions and then precipitated by ethanol Sheared RNA and RT-circulation-primer were used for reverse transcription,the cDNA circularization was performed using CircLigase II (epicenter, CL9021K) for 1 hour at 60 °C