ctDNA of TISF1adult male P25
The library of gDNA was constructed according to the manufacturer's protocols by a QIAamp DNA Kit (Qiagen). For the library of ctDNA constructions, 4bp random nucleolus were added as built-in tags to the both ends of ctDNA after A-tailing of the 45' ends, and further the adaptor were added for HiSeq X Ten sequencing by PCR amplification.The quantities of the library were all measured with Qbit (Thermo Fisher).