DNA-Seq of  P. gingivalis: adult male dental plaque
Take 5-10ug of high-quality genomic DNA, randomly break it into fragments of about 10Kb with Covaris g-TUBE (select 20Kb library according to genome size), and then construct SMRTbell library with DNA Template Prep kit. The constructed SMRTbell library combines sequencing primers and polymerases and adds them to the sequencing chip of the PacBio Sequel platform in a free diffusion manner to prepare for sequencing.