WGS of viral RNA in sample site 4 during Spring
Environmental samples were collected along the Red and Assiniboine Rivers in Winnipeg, Manitoba. Viral DNA and RNA were concentrated from aquatic samples using skimmed-milk flocculation technique and extracted using a Qiagen All-Prep DNA/RNA power microbiome kit. Turbo DNAse and Ambion RNAse III enzymatic treatments were used to enrich viral DNA and RNA fractions respectively. Samples were sent for next generation sequencing to generate to generate paired-end Illumina sequenced reads using NextSeq 550 System and NextSeq 550 System High-Output Kit.