GSM7733950: CRC_77T; Homo sapiens; RNA-Seq
154 CRC tissues removed tumors or biopsy specimens, flash freozen by dry ice, and RNA was harvested using TRIzol (Invitrogen, USA) Total amounts and integrity of RNA were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). For library preparation, the Illumina RNA Library Prep kit (NEBNext® UltraTM) was used. The libraries were tagged with unique adapter indexes. Final libraries were validated on the Agilent 2100 Bioanalyzer, quantified via qRT-PCR, pooled at equimolar ratios, diluted, denatured, and loaded onto an Illumina novaseq 6000.