GSM7147180: Pre-recruitment follicle, Bird 5840; Gallus gallus; RNA-Seq
RNA was extracted using the TRIzol Reagent (Invitrogen). Quality control was performed with Advanced Analytical Technologies, Inc. (AATI) Fragment Analyzer We use poly-A pull-down to enrich mRNAs from total RNA samples (250ng-1ug per sample, RIN>7.8 required) and proceed on library preparation by using Illumina TruSeq chemsitry. Libraries are then sequenced using Illumina NovaSeq 6000 at Columbia Genome Center. We multiplex samples in each lane, which yields targeted number of paired-end 100bp reads for each sample.