GSM7816248: dLsd1∆N/dLsd1∆N 3; Drosophila melanogaster; RNA-Seq
Wing discs from L3 wandering female larvae from dLsd1∆N homozygous mutant and wild-type stocks were dissected in ice cold PBS. A total of 30 wing discs per sample were used to extract RNA using TriZol reagent (Invitrogen). Samples were protected from RNAses using RNAsecure (Ref AM7006 - Ambion). DNA contaminants were eliminated using DNA-free DNA Removal kit (Ref AM1906 - Invitrogen). RNA cleanup and purification were performed using RNeasy Mini Kit (Ref 74104 – QIAGEN) and RNA was resuspended in water. Library preparation was performed using the standard Illumina TruSeq stranded mRNA Library preparation following manufactures instruction