GSM8113929: RJA Group miRNA Sample 3; Mus musculus; miRNA-Seq
miRNA was extracted using the NEB Next® Multiplex Small RNA Library Prep Set for Illumina®. The quality and integrity of the extracted RNA were assessed using an Agilent 2100 Bioanalyzer. Briefly, 3' and 5' adaptors were ligated to the ends of small RNA. First strand cDNA was synthesized followed by PCR enrichment to generate the double-stranded cDNA library. After purification and size selection, libraries with insertions between 18~40 bp were prepared for Illumina sequencing with SE50.