RNA-Seq of Dalbergia odorifera: leaflet of seedlings
Genomic DNA was extracted and fragmented, followed by end-repair and A-tailing. Adapters were ligated to the fragments, and size selection was conducted to obtain appropriately sized fragments. The library was then PCR-amplified, purified, and quantified. Finally, the library's quality was verified before proceeding to high-throughput sequencing, ensuring an unbiased representation of the genome for analysis.