GSM7508644: Stage4_rep3; Spirodela polyrhiza; RNA-Seq
Total RNA was extracted from frond tissues of S. polyrhiza plants at development stages 2 - 6 (n = 3 for stages 2 - 4 and n = 2 for stages 5 - 6), using the Qiagen RNeasy mini kit (Qiagen, Germany) according to the manufacturer's instructions. Samples that met the quality control criterion (RNA integrity number > 0.8) were used for library construction. After quality assessment and RNA quantification, mRNA sequencing libraries were prepared using TruSeq Stranded mRNA Library Prep Kit (Illumina, USA), following the manufacturer's instructions.