GSM7029346: RIP_H1_YTHDC2E317Q_rescue_1; Homo sapiens; RIP-Seq
Cells were lysed in lysis buffer on ice to extract native RNA-protein complexes. The soluble complexes were enriched by YTHDC2 antibodies. RNA was extracted using the Direct-zol RNA MiniPrep Kit (Zymo Research, 11-331). 10 ng of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared following the Smart-seq2 protocol.