ATAC-seq of Schmidtea nova: wild type, bio. sample 1, tech. sample 2
Tagmented DNA was PCR-amplified with 1x KAPA HiFi HotStart Readymix and 100 nM unique dual index P5 and P7 primers compatible with Illumina Nextera DNA barcoding (72C for 5 min, 98C for 30 s, thermocycling for 5 cycles at 98C for 10 s, 63C for 30 s and 72C for 1 min). qPCR was then performed with 1 l to determine remaining PCR cycle numbers (7-13) (see Buenrostro et al. 2015). Purification and double-sided size selection of amplified libraries was done with AMPure XP beads (Beckmann Coulter; starting with a 1.55x volume of XP bead purification, followed by a 0.6x/1.55x double-sided size selection, an additional 0.6x/1.55x double sided size selection was performed if needed).