ONT_BovViromeCNB8
For MinION sequencing, libraries were prepared following the manufacturer's instructions for cDNA amplification. Barcode ligation, assigning one barcode per sample, was initially performed using the ONT Barcode Kit (EXP-NBD196). Subsequently, the library was constructed by combining the amplicons with their ligated barcodes in equal volumes, followed by adapter ligation using the ONT ligation sequencing kit (SQK.LSK109). The constructed library was sequenced on an ONT MinION using R.9.4 flow cells and MinKnow V.3.1.4 software. The adapter-linked libraries were loaded onto FLO-MIN106 flow cells R9.4.1 on the MinION device (ONT, UK). Sequencing was conducted using the MinKNOW V.3.1.4 program with a runtime of 48-72 hours.