GSM7063774: RKO cells, OECtrl (MCS) rep2; Homo sapiens; RNA-Seq
The total RNA was extracted according to the instruction manual of the TRlzol Reagent (Life technologies, California, USA). A total amount of 1 μg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using Hieff NGS Ultima Dual-mode mRNA Library Prep Kit for Illumina (Yeasen Biotechnology (Shanghai) Co., Ltd.) following manufacturer's recommendations and index codes were added to attribute sequences to each sample..