RNA-seq of mus musculus: colon
Colon tissue samples were extracted from the colons of the mice using the Illumi-na TruSeq total RNA kit. Paired-end (PE) 100 bp sequences were produced using an Illumina Novaseq 6000 . The PE sequences were put through quality control, mapped, and analyzed for differential expression using the Hisat2-StringTie-DeSeq2 pipeline default parameters using the method reported in Liu et al., 2023 (10, 12-15). Gene annotations based on Ensembl database records for mouse strain C3H/HeNCr (16). Final gene lists based on significance threshold set at q-value  0.05. The normal-ized read counts from this data were used downstream for differential correlation (DC) analysis.