Comprehensive 3' end mapping of S. frugiperda telomerase RNA forms
S. frugiperda total RNA was guanosine / inosine tailed and reverse transcribed using an oligo-dC reverse primer that contains a 5' adapter. Kinased PCR primers targeting S. frugiperda TR and the oligo-dC reverse primer 5' adapter were used for PCR ampilification using a DNA polymerase that adds non-templated A residues to the 3' ends of the PCR DNA. Nanopore adapters from the SQK-LSK110 kit were ligated according to recommendations and sequenced on a Flongle flow cell. Data were basecalled using Guppy version 6.3.8 and reads that pass the quality threshold are deposited.