GSM7912112: Y1FHUn3; Schizosaccharomyces pombe; RNA-Seq
Total RNA was extracted using a hot phenol method, followed by purification using the RNeasy Mini Kit (Qiagen). Quality of the RNA samples (RIN score >7) was verified on the Agilent 2100 Bioanalyzer using the RNA 6000 kit (Agilent, cat# 5067-1513). 1 μg of each RNA sample was then used for poly(A) selection and library preparation. Poly(A) selection was conducted using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Libraries were prepared with 0.1 μg poly(A) RNA using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs).