GSM8425532: E12.5 dorsal telencephalon, wild type female, scRNA-seq, rep2; Mus musculus; RNA-Seq
The dorsal telencephalon was dissected from four litter- and sex-matched pairs of wild type and Chd8+/- embryos at four time points: embryonic day (E) 12.5, E14.5, E16.0, and E17.5. The medial dorsal telencephalon was removed to avoid capturing the hippocampal primordium. Freshly dissected tissue was immediately dissociated into single cells, then loaded onto the 10x Genomics Chromium Controller for single cell capture. Libraries were generated and indexed using the 10x Chromium Next GEM Single-Cell 3' kit with v2 chemistry, then sequenced using standard Illumina protocols on an Illumina HiSeq 2500, with 26 cycles for read 1 and 98 cycles for read 2.