GSM7946943: RNA ArR_5h_1; Artemia parthenogenetic lineage; RNA-Seq
The total RNA from Artemia cysts was isolated using TRIzol reagent and treated with RNase-free DNase I to remove any contaminating genomic DNA. RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA) Sequencing libraries were generated using a NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA). Library quality was assessed on the Agilent Bioanalyzer 2100 system. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using a TruSeq PE Cluster Kit v3-cBot-HS (Illumina). After cluster generation, the library preparations were sequenced on an Illumina novaseq platform and 150 bp paired-end reads were generated.