GSM7818339: alt-splicing RNA-Seq - Hippocampus Aged Male Replicate 3; Mus musculus; RNA-Seq
RNA was extracted from Trizol using a Trizol/RNeasy hybrid protocol. Briefly, after phase separation, the aqueous phase was mixed with one volume of 70% ethanol and passed through an RNeasy spin column (QIAGEN RNeasy Mini Kit). mRNA libraries were prepared with poly-A selection using the TruSeq Stranded mRNA Library Prep Kit (Illumina 20020594)