ITS2 metabarcoding bulk soil from oil rapseed 100% Ap soil rep 2
DNA was extracted using DNeasy PowerLyzer PowerSoil Kit (Qiagen). The amplification of fungal DNA was carried out in ZALF e.v. WG Microbial Biogeochemistry (Muencheberg, Germany) using the primers ITS86F and ITS4R. Labwork: Julian Ruggaber  Amplicons were sequenced on an Illumina MiSeq instrument with 300bp paired-end reads at LGC genomics GmbH (Berlin, Germany)