GSM7733585: H1836; Homo sapiens; Bisulfite-Seq
Qiagen AllPrep DNA/RNA Mini Kit Briefly, approximately 100ng of genomic DNA was digested using MspI, and Illumina-compatible cytosine-methylated adaptor were ligated to the enzyme-digested DNA. RRBS libraries were then visualized using Bioanalyzer High Sensitivity DNA chips (Agilent, Santa Clara, CA), and those passing QC were subsequently sequenced as 100bp paired-end reads on an Illumina NovaSeq instrument. NuGEN Ovation RRBS Methyl-Seq