GSM6797867: SJ, replicate 1, scRNAseq; Mus musculus; RNA-Seq
The livers obtained from fibrotic liver of S. japonicum-induced mouse and healthy liver of normal mouse. The fresh tissues were stored in the sCelLiveTM Tissue Preservation Solution (Singleron) on ice as quickly as possible. After washed with Hanks Balanced Salt Solution (HBSS) for three times, the specimens were minced into small pieces. To dissociated into single-cell suspensions, the specimens then digested with 3 mL sCelLiveTM Tissue Dissociation Solution (Singleron) by Singleron PythoN™ Tissue Dissociation System at 37 °C for 15 min. After filtered through a 40-micron sterile strainer, the cell was added the GEXSCOPE® red blood cell lysis buﬀer (RCLB, Singleron), which was incubated at room temperature for 5-8 min to remove red blood cells. Centrifugation was performed at 300 × g 4 ℃ for 5 mins to remove supernatant and single cells were resuspended softly in PBS. Single cell suspension was loaded onto microwell chip  and single cell RNA-seq libraries were constructed according to the manufacturer's instructions (Singleron GEXSCOPE Single Cell RNAseq Library