RNA-seq of S. aureus: codY*, PYO, 30m
Total RNA stabilized in RNAprotect and isolated using a Total RNA Plus Purification kit (Norgen) with modifications for S. aureus. Total RNA was treated with TURBO Dnase to remove remaining gDNA and rRNA removed using Ribo-Zero rRNA Removal Kit for Gram-Positive Bacteria (Illumina). RNA libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina sequencing.