WGS of hacat cells at P0 (10X)
cells were harvested and genomic DNA was extracted using NucleoSpin Tissue kit (Macherey Nagel, Dren, Germany). DNA quantification [Quant-iT PicoGreen dsDNA Kit (Invitrogen)], library preparation (Illumina DNA PCR-Free Library Prep Kit using 25ng DNA) and quantification/normalization [KAPA Library Quantification Kit (KapaBiosystems, Wilmington, MA, USA)] were then performed at the GIGA-Genomics platform (University of Liege). Sequencing (30X coverage) was conducted on the Illumina NovaSeq6000 platform (PE150 mode).