BH4_1W_4
Evenly germinated tobacco seedlings were added to one side of two-compartment plates and exposed to one of three treatments: NYG5-emitted VOCs, BH4-emitted VOCs and a control plate without bacteria. Plant samples were collected at three different time points: 24 hours, 72 hours and one week following the start of the experiment. Five replicates per treatment were collected, each containing of at least nine tobacco seedlings. The seedlings were flash frozen in liquid nitrogen. Next, samples were ground to powder in a pre-chilled pestle and mortar filled with liquid nitrogen. 75 mg of each replicate was added to a RNAse/DNase free sterile Eppendorf tube and flash frozen in liquid nitrogen. Total RNA was extracted using the InviTrap Spin Plant RNA Mini Kit 50 (Invitek diagnostics, Berlin, Germany),