MeRIP-seq  of Bos taurus
Firstly, fragmented mRNA was incubated with anti-m6A polyclonal antibody (Synaptic Systems) in IP buffer for 2 h at 4 . Then, immunoprecipitated mRNAs or Input was used for library construction with NEBNext Ultra  Directional RNA Library Prep Kit for Illumina (New England Biolabs, MA, USA). After library quality control, sequencing was performed on the Illumina NovaSeq platform according to the standard protocols.