ChIP-seq using IgG antibody of HA-tagged P. falciparum NF54 at schizont stage
iRBCs were collected at P. falciparum schizont stage and chromatin crosslinked using 1% formaldehyde. Chromatin was sheared using the Covaris S220 ultrasonicator and immunoprecipitated with anti-IgG antibody. DNA was recovered by phenol/chloroform extraction and ethanol precipitation and purified using AMPure XP beads. Libraries were then prepared with the KAPA LTP Library Preparation Kit.