16srRNA Amplicon sequences of bacteria in Strauzia longipennis
PCR amplification from a whole-organism sample, targeting the B_V23 section of bacterial 16S rRNA and using the universal primers 16S_BV2f and 16S_BV3r (Bukin et al., 2019). Barcodes were added to the ends of both forward and reverse primers such that reads originating from each insect specimen could later be separated using their unique combination of barcodes. Libraries were sequenced with an Illumina Miseq (2 x 300 cycles). The two paired ends of each read have been stitched together (this step was necessary to demultiplex samples) and primer and barcode sequences have been removed.