miRNA-Seq of blood from Ward Patient 2
This study utilized miRNA sequencing analysis using the Illumina NovaSeq6000 platform. Total RNA, including small RNA, was isolated using the TRIzol Plus RNA Purification Kit, with sample quality assessed via concentration determination using the Fragment Analyzer. The NEBNext Multiplex Small RNA Library Prep Kit for Illumina was employed for sample processing, following a standard operating procedure for NEBNext Small RNA library prep. Despite lower-than-expected small RNA library yields, additional PCR cycles were incorporated in an attempt to improve yield, with subsequent confirmation of consistent product sizes. Primary data analysis involved image analysis, base calling, and quality checks conducted through the Illumina data analysis pipeline RTA3.4.4 and BclConvert v3.10.5.