MiSeq of reef picoplankton
Partial small subunit ribosomal RNA genes were amplified from bacteria and archaea in the DNA using 515FY (31) and 806RB (32) dual indexed primers with 20 l reactions consisting of 5 l GoTaq Flexi 5x buffer, 2.5 l of 25 mM MgCl2, 1 l of 10 mM dNTPs, 1 l of each forward and reverse primer, 1 l of DNA template, with sterile water as the remaining volume.