RNA-Seq of homo sapiens: adult male blood NK cells stimuls LLL
Total RNA was extracted from collected samples with TRIzol (Invitrogen, Carlsbad, USA). Treatment with DNase was performed to avoid the influence by genomic DNA (Thermo Fisher Scientific, MA, USA). The following conversion of total RNA into a cDNA was performed using NebNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs, MA, USA) according to the manufacturer's instructions. Adaptor ligation, and addition of indexes were conducted using NEBNext Multiplex Oligos for Illumina (New England Biolabs, MA, USA). The final average fragment size was 300 bases.