Examination of 16S rRNA gene amplicons extracted from nasal swabs of beef calves in Texas: A Comparative Study between Apparently Healthy and Calves Diagnosed with Bovine Respiratory Disease.
PCR-grade water was utilized to construct the 16S rRNA gene library (V4 16S rRNA gene) following the protocol outlined by the Schloss lab and Centeno-Martinez et al., 2022. Following this, the 16S rRNA gene amplicons were sequenced using Illumina MiSeq (2x250, paired-end) technology.