GBS library constructed from genomic DNA of multiple individuals in the bird genera Sphyrapicus, Zonotrichia, and Leiothlypis - Plate 2
Genomic DNA was extracted from blood and tissue samples using a standard phenol-chloroform extraction procedure. The samples were genotyped using a genotyping-by-sequencing (GBS) procedure first described by Elshire et al. (2011) with modifications described in Alcaide et al. (2014) and Geraldes et al. (2019). We selected for 400-500 bp fragments.