Field application of glycerol to enhance reductive dechlorination of chlorinated ethenes and its impact on microbial community
DNA samples were sequenced in duplicate. Two sequential PCRs were performed to amplify the DNA from the V4 region of the eubacterial 16S rRNA gene was performed with primers 515F and 802R . The PCR cycle conditions were as follows: 1st  PCR: 95 C for 3 min; 10 cycles at 98 C for 20 s, 50 C for 15 s, 72 C for 45 s, and a final extension at 72 C for 1 min; 2nd PCR: 95 C for 3 min; 35 cycles at 98 C for 20 s, 50 C for 15 s, and 72 C for 45 s, with a final extension at 72 C for 1 min. The concentration of purified PCR products was detected using a Qubit 2.0 fluorometer.