Macrogenomic sequencing of porcine intestinal contents: langtang colon
The frozen contents of the jejunum and colon were thawed on ice, and total genomic DNAs were extracted by a Quick Gel Extraction Kit (Qiagen, Hilden, Germany) . We then measured the DNA concentration and purity with a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE). The total DNAs were subjected to library construction through DNA-fragmentation, end-repair, adapter-ligation, and unbiased PCR amplification using NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, Ipswich, MA). Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA) was used for quality control of the DNA libraries, and the qualified libraries were sequenced with an Illumina platform.