Examination of 16S rRNA gene amplicons extracted from nasal swabs of beef calves in Texas: A Comparative Study between Apparently Healthy and Calves Diagnosed with Bovine Respiratory Disease.
The DNA extracted from 20 known bacterial strains was employed to create the 16S rRNA gene library (V4 16S rRNA gene), following the protocols outlined by the Schloss lab and Centeno-Martinez et al., 2022. Subsequently, the 16S rRNA gene amplicons underwent sequencing using Illumina MiSeq (2x250, paired-end) technology.