Virome sequencing of Lettuce Big Vein Disease infected Lactuca sativae
Leaf material was grinded to a fine powder using mortar and pestle and used as input material for total RNA isolation using the RNeasy Plant Mini Kit (Qiagen). The isolated total RNA was used for cDNA synthesis using the Maxima H Minus Double Stranded cDNA Synthesis Kit using random hexamer primers (Thermo Scientific). For library construction the Ligation Sequencing Kit SQK-LSK110 was used in combination with the Native Barcoding Kits EXP-NBD104 and EXP-NBD114 (Oxford Nanopore). The constructed sequencing libraries were sequenced using the MinION FLO-MIN106D flowcells (Oxford Nanopore) during a period of 72 hours