AMPLICON
PCR amplifications were performed on an approximately 550 bp fragment of the hypervariable V3-V4 region of 16S rRNA gene, using the  primer set Bakt_805R (5'-GACTACHVGGGTATCTAATCC-3') and Bakt_341F (5'-170 CCTACGGGNGGCWGCAG-3') specific for Bacteria.