Metagenome of river water: site4A
The quality of the DNA was assessed using an Agilent 5400 Bioanalyzer, fragmented using sonification and the polished ends were ligated to Illumina adaptors that were amplified using index oligos P5 and P7. The Agilent 2100 Bioanalyzer and qPCR were used to assess the size distribution and concentration of the libraries constructed.