miRNA-Seq of homo sapiens: iMSC derived EV
The purification of total EV-RNA was carried out by the exoReasy kit (Qiagen, Germany). RNA purity was detected using the NanoPhotometer spectrophotometer (IMPLEN, USA), concentration was measured using Qubit RNA assay kit in Qubit 2.0 fluorometer (Life, USA), and integrity was assessed using the RNA Nano 6000 assay kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, USA). Sequencing libraries were generated using NEBNextMultiplex small RNA library prep set for Illumina (NEB, USA). Library quality was assessed on the Agilent Bioanalyzer 2100 system using DNA high sensitivity chips (US). The library was sequenced on an Illumina Hiseq 2500/2000 platform and 50 bp single-end reads were generated.