RNA-Seq of rattus norvegicus: adult female hippocampus
The quality of RNA was determined by using an RNA 6000 Nano LabChip Kit and an Agilent Bioanalyzer 2100 (Agilent, USA). A total amount of 1 g of RNA per sample was used as input material for the RNA sample preparations. mRNA from eukaryotic organisms was enriched using oligo(dT) beads. Subsequently, sequencing libraries were generated using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, USA) following the manufacturers recommendations. Complete cDNA libraries of 250300 bp in length acquired from samples were subjected to sequencing on an Illumina system (PE150, 20 M reads per sample).