Analysis of 16S rRNA gene amplicons from nasal swabs of beef calves in Idaho: Comparative Study between Apparently Healthy and Calves Diagnosed with Bovine Respiratory Disease
The DNA extracted from 20 known bacterial strains was utilized to construct the 16S rRNA gene library (V4 16S rRNA gene), following the protocols established by the Schloss lab and Centeno-Martinez et al., 2022. Subsequently, the 16S rRNA gene amplicons were sequenced using Illumina MiSeq (2x250, paired-end) technology