ddRADseq raw data of Calotriton arnoldi
ddRADseq genomic libraries were produced following Petersons et al. (2012) protocol for 154 specimens. In short, we double-digested 500 ng of genomic DNA, using a pair of rare and common restriction enzymes (Sbf1 and Msp1, respectively). The resulting fragments were ligated with barcoded Illumina adapters and, afterwards, were pooled together in batches of eight samples. Fragments were then size-selected for a range between 415  515 bp and sequenced on an Illumina NextSeq 500, for 75 bp single-end reads. Raw Illumina reads were demultiplexed using ipyrad v.0.9.62 (Eaton & Overcast, 2020).