ChIP-seq of Saccharomyces cerevisiae: Thiolutin timecourse
Chromatin IP samples using FLAG M2 antibody (Sigma) for FLAG-tagged Rpb3 from control or thiolutin treated yeast chromatin. Cells were untreated, or treated with thiolutin (in DMSO) at 10 g/mL final concentration in the medium for 1, 2, 4, or 8 minutes or with DMSO (vehicle for thiolutin).  for 8 minutes. Chromatin samples included spike-in chromatin from Schizosaccharomyces pombe also including FLAG-tagged rpb3+.