shotgun metagenome of reef sediment: sediments grazed by sea cucumbers
At the Harvard Bauer Core 9 ng of DNA from each sample was simultaneously fragmented and tagged with Illumina adapter sequences (tagmentation) with the Illumina DNA library preparation kit (Illumina Inc. San Diego, CA) creating ~350bp inserts. Tagged inserts were enriched and 10 bp barcodes sequences were appended with 12 cycles of amplification to allow multiplexing. After barcoding, libraries were purified with magnetic bead-based cleanup using Illumina Sample Purification Beads (Illumina Inc. San Diego, CA). Resulting libraries were assessed using a 4200 TapeStation (Agilent Technologies, Santa Clara, CA) and quantified by qPCR (Roche Sequencing, Indianapolis, IN). Libraries were pooled and sequenced on one lane of an Illumina NovaSeq X Plus S4 flow cell using paired-end 150 bp reads