M confusa antennea F3
Pupae were allowed to eclose at 26oC and 80% humidity under a 12:12 day:night regime. pairs of antennae were homogenised with RLT buffer by repeated aspiration with a 21-gauge needle, and RNA was extracted using a Qiagen RNeasy kit, following the manufacturers instructions, including treatment with Qiagen RNase-free DNase to remove any remaining DNA. To achieve sufficient concentrations, three individuals were pooled in each sample according to their estimated RNA concentrations, with three-replicates per sex per species for Mc. polymnia, T. harmonia, Me. confusa. Sufficient samples of G. morgane were not available at the time of sampling. Polyadenylated Illumina RNAseq data (125 bp x 2) was carried out by University of Liverpool Centre for Genomic, for a total of nine samples with an average of ~38 million fragments per sample.