RNA-SEQ of homo sapiens: adult female control stem cells2
one microgram of total RNA was used as starting material. The ribosomal RNA was depleted using the RiboZero Gold that was included in the kit. The resulting RNA samples were fragmented and converted to double-stranded DNA for sequencing. Individual libraries were uniquely indexed using TruSeq RNA UD Indexes (Illumina, Cat. No. 20020591) and pooled in equimolar ratio