AmpliSeq of Homo sapiens: invasive ductal carcinoma
Next-generation sequencing libraries were prepared from 60 ng genomic DNA (30 ng of input per pool) with an AmpliSeq PLUS kit (Illumina, Singapore) using an AmpliSeq Custom DNA Panel (Illumina, San Diego, CA, USA) for TP53, HDM2, HDM4, PARP1, BARD1, ATR, RAD50, NBN, MYC, CDKN2A, H2AFX, ATM, CHEK1, BRCA2, RAD51, PALB2, BRIP1, PPM1D, BRCA1, and CHEK2 (93.39% coverage, number of amplicons = 747, amplicons < 275 bp) as per the manufacturers protocol (AmpliSeq for Illumina On-demand, Custom, and Community Panels Reference Guide; Document # 1000000036408 v09).