DNA2019-522_Toolik time series_20-Sep-2019_Toolik_lake water_16m
Bacteria-specific PCR primers were used for amplification of the V4 region of 16S rRNA genes with forward primer 515f  and reverse primer 806r each linked to 8-basepair barcodes and Illumina adapters (Illumina adapter - 8bp barcode - 10bp pad - 2bp linker - 16S primer (515f: AATGATACGGCGACCACCGAGATCTACAC-NNNNNNNN-TATGGTAATT-GT-GTGCCAGCMGCCGCGGTAA; 806r: CAAGCAGAAGACGGCATACGAGAT-NNNNNNNN-AGTCAGTCAG-CC-GGACTACHVGGGTWTCTAAT)