Amplicon sequencing of VH and VL domains of recombinant human Fab clones from screening plate RS016
VH and VL domains of the Fab clones were first amplified from single, inoculated E. coli colonies with primers containing custom well indexes, followed with pooling of 96 samples from the same plate and purification of DNA of pooled samples with GeneJet PCR purification kit. The purified samples were then amplified again using TruSeq primers, containing another set of indexes (96 plate indexes) and Illumina sequencing compatible adapters. Libraries were then size selected with agarose gel extraction, followed with quantification with Qubit and Bioanalyzer.