RNA-seq data from Eulittoral Sandy Beach, Mediterranean Sea: HDPE contact sediments
The total DNA was extracted from samples by using the NucleoMag DNA/RNA water kit (Macherey Nagel GmbH & Co. KG, Dren, Germany). The DNA was used to generate amplicons that cover V3 and V4 hypervariable regions of bacteria and archaea 16S rDNA. . Indexed adapters were added to the ends of the 16S rDNA amplicons by limited cycle PCR. Sequencing libraries were validated using a DNA chip for the Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA), and quantified by Qubit and real-time PCR (Applied Biosystems, Carlsbad, CA, USA). DNA libraries were multiplexed and loaded on an Illumina MiSeq instrument according to the manufacturers instructions (Illumina, San Diego, CA, USA). Sequencing was performed using a 2x250 paired-end configuration; image analysis and base calling were conducted by the MiSeq Control Software on the MiSeq instrument.