ONT seq of Pf_T2
size selected DNA was evaluated for quantity and quality using a Qubit fluorometer (ThermoScientific, Waltham, MD) with a high sensitivity genomic DNA fluorescent kit and a TapeStation 4150 (ThermoScientific) using the genomic DNA screentape and ladder for fragments >50 Kb. Following HMW DNA quality checks, Oxford Nanopore Technologies (ONT; Oxford, UK) sequencing libraries were prepared using V14 kit with duplex reads (SQK-LSK114). The resulting libraries were checked for quality and quantity using the TapeStation as described previously. The ONT library was sequenced for ~72 hours on a ONT R10.4.1 flow cell using a MinION device. Reads of an estimated <1000 bp were filtered using the sequencing software MinKNOW (v23.04.6), real-time basecalling was disabled, and POD5 files were chosen to be the output. POD5 files were used as input into the Dorado (v0.3.3; ONT) basecaller. The basecalling was performed using the super accurate (SUP) basecalling model dna_r10.4.1_e8.2_400bps_sup@v4.2.0.