amlicon sequencing of T71am73 gene1 pORTPHAGE
The sample was first pooled with 20ng from each sample, in this case 10 samples with 20ng each was 200ng total.The sample pool was then fragmented by covaris sonication, model S220, see attached Quick Guide protocol. Fragment size 250bp. The pool was then prepared into a library using the xGen cfDNA and FFPE kit (formerly called Prism) by IDT, see attached protocol. Bead size selection using the protocol was set for 130-300 bp before adapters, (250-420 bp with adapters).The library was sequences by Novogene Singapore using the Novaseq 6000 system with an S4 cartridge and 2x150 or PE150, 1GB of data was generated for the pooled sample.