snRNA-seq of taxus leaves
Single-cell suspension was added to the 10x Chromiumchip accordingtothe instructions for the 10X Genomics Chromium Single-Cell 3' kit (V3), withtheexpectation of capturing 8,000 cells. cDNA amplification andlibraryconstruction were performed according to standard protocols. Librariesweresequenced by Illumina NovaSeq 6000 sequencing system(double-endsequencing, 150 bp) at a minimum depth of 20,000 reads per cell.