ITS-Seq of fungus: swine wastewater
The TIANNAMP DNA Kit (TIANGEN, Beijing, China) was used to extract DNA. The quantity and quality of DNA were evaluated via 1% gel, NanoDrop 2000.The 16S rRNA gene of bacteria (in the V3V4 region) was amplified using the primer pairs 515F and 806R , and 528F and 706R.