RNASeq of KPC cells: Non-targeting Hairpin, Replicate 1
RNA was extracted from biological triplicates of Upf1 KD and control KPC cells with an RNeasy Mini kit (QIAGEN) and analyzed by Bioanalyzer for quality control and screened for RNA integrity by RIN. cDNA libraries were generated from the samples by automated stranded RNA-Seq library prep with poly A selection and sequenced by an Illumina NovaSeq 6000 using an S2 100 cycle v 1.5 flow cell at a depth of 30 million reads per sample.