ML25-006
2x301 base pair amplicons were obtained spanning the V4 region of the 16S rRNA gene. Primers used were 515F (GTGCCAGCMGCCGCGGTAA) and 805R (GGACTACHVGGGTWTCTAAT). The reads were demultiplexed, contaminants removed, and adapters trimmed using BBDuk version 37.90. Forward and reverse reads were split using khmer version 2.1.1 and screed version 1.0.