16S amplicon sequencing of lake water: Lake Geneva_lexplore_Jul_12m
To analyze the community structure of planktonic bacteria, 16S rRNA gene amplicon sequencing was performed for the filter samples collected at a certain depth. From the Sterivex filter samples, DNA was extracted using the PowerWater Sterivex DNA Isolation Kit (Qiagen, Hilden, Germany). From the DNA samples, sequencing libraries were prepared according to the Illumina 16S Metagenomic Sequencing Library Preparation protocol and then subjected to Illumina MiSeq v3 or NovaSeq 6000 sequencing. The resulting reads were processed with DADA2 followed by Silva (nr99/v138.1).