WGS of Lagria hirta: Pool of 6 egg clutches (20-30 eggs per clutch)
Genomic DNA was extracted using the Qiagen Genomic-tip 20/G Kit (Qiagen) following the instructions from the manufacturer. End-DNA repair and library preparation was done using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, Ipswich, USA) and the Ligation Sequencing Kit V14 (SQK-LSK114; Oxford Nanopore Technologies, Oxford, UK) followed by a clean-up step with AMPure XP beads (Beckman Coulter).