Illumina MiSeq metabarcoding of seawater: 12S, 18S, COI fragments
Library construction proceeded by tagging each PCR pool (for three markers, 12S, 18S, COI) with a combination of Illumina barcodes through a second amplification process. Sequencing of the libraries occurred using Illumina MiSeq V2 chemistry to produce 2 x 250-bp paired-end reads.