RNA-seq of Aedes aegypti: adult P_sel_2
Total RNA was extracted from pools of whole adult mosquitoes (5 male and 5 female per pool) at age 5-7 days post eclsosion. Mosquitoes were homogenized in 200L of RLT buffer and beta-mercaptoethanol, and RNA was then extracted using a RNeasy Mini Kit (Qiagen, Cat: 74104). An Illumina sequencing library was constructed for each RNA sample according to the manufacturer and sequenced on the Illumina platforms with paired-end 150 bp (PE 150) by Novogene Co., LTD.