RNA-Seq of Fro3
Sequencing library preparation for next-generation sequencing involves converting DNA or RNA into a form suitable for high-throughput sequencing by fragmenting the nucleic acid, repairing ends, adding A-tails, ligating adapters, size-selecting, and amplifying the fragments through PCR. The resulting library is then validated for quality and size before being sequenced on a suitable platform. This process is critical for generating accurate and reliable genomic or transcriptomic data.