Whole-exome sequencing of homo sapiens: XP-V (complemented with POLH) SV40 skin fibroblast - treated with 120 kJ/m2 UVA
The two strains were plated to confluence between 1-3 x 103 cells in 100 mm dishes. After 10 to 15 days of growth, colonies were randomly selected and transferred to 96-well plates. The transfer was performed manually, using a 200 L micropipette and with the EVOSTM XL Core Imaging System microscope (ThermoFisher, Waltham, MA, USA). The growth and expansion of each colony was monitored individually and daily, and as soon as they reached adequate confluence, a new expansion was carried out to freeze the stock and perform DNA extraction with the Blood and Cell Culture DNA Mini Kit (Qiagen, Hilden, Nordrhein-Westfalen, Germany), QIAamp DNA Mini Kit (Qiagen) or NucleoSpin Tissue mini kit for DNA from cells and tissue (Macherey-Nagel, Dueren, Germany) for exome sequencing (input 5 g of genomic DNA).  Library preparation was performed using the Nextera Rapid Capture Custom Enrichment or Nextera DNA Flex, Illumina kits, Exon capture was performed with xGen Exome Research Panel v1.0 or xGen Exome Research Panel v2.0, both IDT. Libraries were sequenced in paired-end mode on the Illumina HiSeq 2500 or NovaSeq platforms.