RNA-Seq of PC3 Cell Line
RNA was extracted using the RNeasy kit (Qiagen), followed by reverse transcription by using High-Capacity cDNA Reverse Transcription kit (Thermo Fisher Scientific) and DNase digestion step using RNase-Free DNase Set (Qiagen). Quality control of all RNA samples was performed using a 2200 Tapestation and High-sensitivity RNA screentape (Agilent) to measure the quality of the purified RNA, and only with RIN values >7.9 were processed, with 1ug of total RNA used as initial input. Libraries were then prepared using manufacturers standard procedures (Illumina TruSeq stranded mRNA).