RAD sequencing of Eucera pruinosa: thoracic tissue
Genomic DNA was extracted from ethanol-preserved samples using a standard phenol:chloroform extraction protocol. DNA was digested with the restriction enzymes EcoRI and MspI, and barcodes/adaptors were ligated to the sheared ends. Fragments were size-selected using a BluePippin (Sage Science Inc, Beverly, MA) targeting an insert size of 350 bp, amplified in four separate high-fidelity PCR reactions, purified with AMPure XP beads, then sequenced on a single lane of an Illumina Hiseq 2500 machine.