RNA-seq of Sus scrofa: A151R-deleted ASFV mutant infection 12h
PAMs were infected with ASFV-A151R or ASFV-WT at an MOI of 1. At 12, and 20 hpi, the cells were used to extract total RNAs by using TRIzol reagent (catalog no. 15596026; Invitrogen) according to the manufacturers instructions. RNA quantification and qualification were assessed using the RNA Nano 6000 assay kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). The mRNAs with poly(A) tails were enriched and purified from the total mRNAs by poly(T) oligo-coupled magnetic beads. RNA-seq libraries were prepared and assessed by AMPure XP system kit (Beckman Coulter, Beverly, USA) in the Agilent Bioanalyzer 2100 system. Subsequently, the RNA-seq libraries were sequenced on an Illumina Novaseq platform (Nova gene, China). KEGG enrichment analysis of DEGs was performed using Cluster Profiler (3.4.4).