WGBS of autotetraploid Carassius auratus: hatching
Whole-Genome Bisulfite Sequencing (WGBS) library construction involves extracting high-quality genomic DNA, fragmenting it into 200-500 bp pieces, and performing end repair and adapter ligation. The DNA is then treated with bisulfite to convert unmethylated cytosines to uracils while preserving methylated cytosines. The treated DNA is PCR amplified, purified, and subjected to quality control checks for fragment size distribution and concentration. Finally, the library is sequenced using a high-throughput platform, enabling comprehensive analysis of DNA methylation patterns across the genome.