WGS of Mycobacterium fortuitum MF GZ001 clinical isolate
The qualified libraries were sequenced pair-end PE150 on the Illumina HiseqXten/Novaseq/MGI2000 System. For the PacBio sequencing library, 5-10g genomic DNA was sheared into 10-15 Kb fragments 14 using a g-TUBE device. The library was sequenced using the Sequel II sequencing platform. PacBio reads were assembled using HGAP4/Falcon of WGS-Assembler 8.2. And then we recorrect the genome with software Pilon using previous illumine data or 25 Quiver using Pacbio reads