RNA-seq of pKD_MORC P. falciparum NF54::pCRISPRint at 36h
P. falciparum parasites were extracted at 36 hpi following saponin treatment. RNA was extracted with TRIzol LS Reagent (Invitrogen) followed. RNA samples were visualized by RNA electrophoresis and quantified on Synergy HT (BioTek). mRNA was then purified using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB). Libraries were prepared using NEBNext Ultra Directional RNA Library Prep Kit (NEB) and amplified by PCR with KAPA HiFi HotStart Ready Mix (Roche).