DNA-seq of Neofabraea malicorticis: NGS
The 350 bp paired-end library was constructed according to the manufacturers introductions (Illumina) and sequenced on the Illumina Novaseq 6000/ Illumina Novaseq X plus platform by Berry Genomics Company (http://www.berrygenomics.com/. Beijing, China). Basically the libraries were prepared following these steps: DNA fragmentation by sonication, end-polishing of the DNA fragments, A-tailing and ligation with the full- length adapters for Illumina sequencing, PCR amplification, and purification of PCR products (AMPure XP bead system). The libraries were analyzed for size distribution using an Agilent 2100 Bioanalyzer and were quantified using real-time PCR.