SequencingRun1_SK7622_colony
A library of single-codon substitutions in the rnc gene was constructed using inverse PCR. The library was transformed into NEB 5-alpha cells. Plasmids were prepped from NEB5alpha colonies that grew overnight on bioassay plates. That plasmid library was transformed into SK7622 cells. Library were prepped from SK7622 colonies that grew overnight on bioassay plates. We determined the allele frequencies by deep sequencing.