pooled scRNA library for Batch X002 Well RP1C2W3 Lane L004
Single-cell RNA-seq libraries were generated using the 10x Genomics Chromium 3‚Äö√Ñ√¥ Single Cell Gene Expression assay (#1000121) and Chromium Controller Instrument according to the manufacturer‚Äö√Ñ√¥s published protocol with modifications for cell hashing (Stoeckius et al., 2018). For each batch of samples, 100,000 cells from 8 hashedsamples with a distinct Hashtag Antibody were pooled into Pool 1; 8 additional samples were pooled using the same method into Pool 2. Roughly 20,000 cells from a Leukopak healthy control were also labeled with a distinct TotalSeq‚Äö√ë¬¢-A Hashtag Antibody, and were spiked into each pool to serve as a batch control. From each pool, 64,000 cells were loaded into each well of a Chromium Single Cell Chip G (10x Genomics #1000073) (8 wells per chip), targeting a recovery of 20,000 singlets from each well. Gel Beads-in-emulsion (GEMs) were then generated using the 10x Chromium Controller.