scRNA-seq of CRNeoblast
To isolate CRNeoblasts, dissociated cells at a density of 1-5106 cells/mL were stained with 1:500 (5 ?M) CellRox Green (Invitrogen, Cat# C10444) and 1:7000 (143 nM) LysoTracker Deep Red (Invitrogen, Cat# L12492). Cells were first gated by forward and side-scatter, then a final gate around CellRox Green high and LysoTracker Deep Red low identified the CRNeoblast population