WGS of Lachnospiraceae: Lach1_Mix
DNA extraction from bacterial cultures is performed using the QIAamp PowerFecal Pro DNA Kit. Illumina compatible libraries are generated using the QIAseq FX Library Kit and sequencing runs are performed on the Illumina NextSeq platform in the Functional Genomics Facility at UChicago. NextSeq runs are performed on 2150 Paired End reads cassettes to generate 1,000,000-3,000,000 reads per isolate. SPADES and prokka are used for assembly and annotation of bacterial whole-genomes.