46s rDNA of mus musculus: feces
Amplicons were extracted from 2% agarose gels and purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, U.S.) according to the manufacturers instructions and quantified using ABI StepOnePlus Real-Time PCR System (Life Technologies Foster City, USA). Purified amplicons were pooled in equimolar and paired-end sequenced(PE280) on an Illumina platform according to the standard protocols.