Whole genome sequencing of Asian green mussel, Perna viridis
Generation of a SMRTbell library: appropriately sized double-stranded DNA fragments of sample were generated by random shearing of DNA. Then SMRTbell library was produced by ligating universal hairpin adapters onto double-stranded DNA fragments. The hairpin dimers formed during this process are removed using Ampure PB bead and size selected using Bluepippin. After the size selection and AMPure PB purification steps, sequencing primer is annealed to the SMRTbell templates, followed by binding of the sequence polymerase to the annealed templates.