RNA-Seq of 14305-001 leaves at 5 days post inoculation with mock solution, biological replicate 1
Total RNA was extracted using a Cetyltrimethyl Ammonium Bromide (CTAB)-based extraction protocol. RNA concentration and purity were evaluated using a Qubit fluorometer (Thermo Scientific) and a NanoDrop 2000c Spectrophotometer (Thermo Scientific), respectively. Sequencing libraries were prepared using the Illumina TruSeq RNA sample preparation kit v.2 (Illumina, CA, USA). Quality control of size and purity of the final libraries was assessed using the High Sensitivity DNA kit on a Bioanalyzer 2100 (Agilent Technologies, CA).