16s RNA of Rattus norvegicus
Different faecal samples were collected and total genomic DNA was carefully extracted from samples weighing 150-200 mg using a specialised faecal DNA extraction kit.The purity and concentration of the isolated DNA was assessed by gel electrophoresis and subsequently stored at -80C. The DNA was extracted from the samples using a special kit. The V3-V4 region of the bacterial 16S rDNA gene was amplified by PCR. PCR products were quantified using a Qubit@ 2.0 fluorometer and an Agilent Bioanalyzer 2100 system. Whole genome sequencing was performed on the Illumina Hiseq 2000 platform and data processing was performed using QIIME version 1.8.0.