miRNA sequencing of leaves from child regions of walnut
Small RNA was isolated from early walnut tissue from juvenile and adult zones using CTAB+Edler RN40 (Edler, RN40)/TRIzol. After extraction, small RNA was verified for purity and prepared for adapter attachment process. To convert short RNA into cDNA, the First Strand Synthesis Reaction buffer, Murine RNase Inhibitor, and M-MLV Reverse Transcriptase (RNase H-) reagent kit were used. The short RNA library was created and PE150 sequenced using the Illumina NovaSeq 6000 (Illumina, San Diego).