NMB006
DNA was extracted using  Qiagen DNeasy PowerSoil HTP 96 kit. Bacterial DNA content was measured using a 16S rRNA quantitative PCR using a plasmid containing E. coli 16S gene as the standard. 16S ribosomal RNA (rRNA) V3V4 regions were amplified, and then the amplified libraries were sequenced on an Illlumina MiSeq instrument with miseq reagent kit v3-600 cycle to capture paired end (2x 300bp) sequences.