RNA-Seq of AhR KO-2
Total RNA was isolated from the cells using the Qiagen RNeasy Mini Kit (Qiagen, cat# 74104). For each sample, 4 ug of total RNA was then used in Illuminas TruSeq Stranded mRNA Library kit (Cat# 20020594). Libraries were sequenced on Illumina NextSeq 500 as paired-end 42-nt reads. Sequence reads were analyzed with the STAR alignment  DESeq2 software pipeline described in the Data Explanation document.