RNA-Seq of Exaiptasia pallida: the interaction between starvation and symbiosis
Aposymbiotic and symbiotic anemones were bisected to create identical clones. Halves were separated and allowed to heal for at least two weeks. After healing, one individual of each clonal pair was fed regularly and the other was starved for two weeks. Total RNA was extracted from each anemone using the RNAqueous Total RNA Isolation Kit (Invitrogen). RNA quantity and integrity were quantified using a NanoDrop ND-1000 Spectrophotometer. Sample aliquots were normalized to a concentration of 11.4 ng/l in 30 l and submitted to the University of Texas at Austin Genomic Sequencing and Analysis Facility for TagSeq library development and sequencing on a NovaSeq6000 SR100. Replicate lanes of each sample were concatenated to produce the final fastqs presented here.