RNA-Seq of L. Sativa plants with different phosphate concentration and AM symbiosis
Total RNA was isolated from lettuce leaves and roots by using Logemann method. Contaminant genomic DNA was removed from each RNA sample by enzymatic digestion using DNAse I (Roche, Basil, Switzerland) following the manufacturers instructions. The RNA concentration of each sample was measured using the Qubit RNA BR Assay kit (Applied Biosystems Invitrogen, Life Technology Corporation, Eugene, OR, USA) and RNA integrity was evaluated by ribosomal RNAs (rRNA) ratio in a 2% TAE agarose gel. cDNA libraries were obtained using the TruSeq RNASeq Sample Prep kit, according to the manufacturers protocol (Illumina Inc., San Diego, CA, USA), from three biological replicates of leaves and roots samples of L. sativa harvested from HiP/-AM, LoP/-AM and LoP/+AM plants. All libraries were sequenced using Illumina HiSeq 2500, yielding paired reads of 150 nucleotides length