wild type sample 6
The process began with the enrichment of RNA using Oligo (dT) beads, followed by fragmentation of the enriched RNA into smaller pieces using a fragmentation buffer, then reverse-transcribed into cDNA and purified using a QiaQuick PCR extraction kit (Qiagen, The Netherlands) and subsequently ligated to sequencing adapters compatible with Illumina platforms. After adapter ligation, the products were size-selected through agarose gel electrophoresis, PCR amplified, and finally sequenced on the Illumina NovaSeq6000.