10x Genomics scRNAseq of mouse placental tissue, ozone exposed
10x Chromium Next GEM 3 protocol was used to create 3 libraries for sequencing. Briefly, cells were lysed and barcoded within each GEM prior to first strand cDNA synthesis, also within each GEM. After GEMs are broken, cells are pooled prior to library completion as described in the manufactures protocol. Library quality was assess after cDNA synthesis and after completion on the BioAnalyzer using a DNA High Sensitivity Chip (Agilent). Prior to sequencing, Agilent Tape Station 4200 and Invitrogen Qubit 4.0 reagents were used determine final library concentrations prior to dilution, normalization and pooling at 4nM. qPCR was used to determine cluster efficiency, before loading libraries into NovaSEQ machines.