single-cell RNA-Seq of homo sapiens: adult female high grade serous carcinoma tumor
Fresh tumor chunks were dissociated to single cells using the Miltenyi Human Tumor Dissociation Kit and the GentleMACS dissociator, followed by cryopreservation in liquid nitrogen. Thawed dissociated cells were submitted for library preparation using the 10X Genomics Chromium Next GEM Single Cell 3' Reagent Kit v3.1. Libraries were sequenced on an Illumina NovaSeq 6000 instrument.