DNA-Seq of Fritillaria ussuriensis:  etiolated seedlings
Approximately 1 microgram of mitochondrial DNA was sonicated to fragments with the length of around 500 bp using the Covaris M220 system, and the sonicated DNA was purified using a TIANgel Midi Purification Kit. After constructing sequencing libraries through the NEBNext Ultra DNA Library Prep Kit, these libraries could be sequenced using the Illumina NovaSeq 6000 platform.