Whole genome sequencing of Kingella kingae isolated from patients
Genomic DNA was extracted from pure cultures using a Qiagen DNAeasy Tissue Kit according to the manufacturers instructions (Qiagen, Germantown, MD). DNA libraries were prepared using the Nextera XT kit (Illumina, San Diego, CA) according to the manufacturer protocol and sequenced with 150bp single-end reads using an Illumina NextSeq platform (Illumina, San Diego, CA).