RNA-Seq of yeast W303: WT
Total RNAs were isolated from whole cell lysate using miRNeasy kit (QIAGEN) and DNase-treated (Lucigen/Epicentre). rRNAs were removed using QIAseq FastSelect - rRNA Yeast kit (QIAGEN) and sequencing libraries were prepared using TruSeq Stranded mRNA Library Prep kit (Illumina).