Analysis of 16S rRNA gene amplicons from nasal swabs of beef calves in Idaho: Comparative Study between Apparently Healthy and Calves Diagnosed with Bovine Respiratory Disease
PCR grade water was employed to assemble the 16S rRNA gene library (V4 16S rRNA gene) following the protocol outlined by the Schloss lab and Centeno-Martinez et al., 2022. Subsequently, the 16S rRNA gene amplicons underwent sequencing using Illumina MiSeq (2x250, paired-end) technology.