WGS of Lagria hirta: Ovipositor and accessory glands from 5 females
Genomic DNA from the preserved organs was extracted per individual after removing the fixative and homogenizing the tissue in liquid nitrogen. The MasterPureTM complete DNA and RNA isolation Kit (Epicentre Technologies) was used as indicated by the manufacturer, including an additional incubation step at 37 for 30 min with 4  lysozyme (100 mg mL-1) before protein precipitation. The nucleic acids were re-suspended in Low TE buffer (1:10 dilution of TE) and pooled together. DNA library preparation was done using a Nextera XT DNA Library Prep Kit (Illumina). Sequencing done by CeGaT GmbH (Tingen, Germany).