16S rRNA Gene Amplicons Analysis of Nasal Swabs from Beef Calves in Colorado: Comparison Between Apparently Healthy and Bovine Respiratory Disease Diagnosed Calves
The extracted DNA from 20 known bacterial strains was utilized to construct the 16S rRNA gene library (V4 region) following the protocol outlined by the Schloss lab and Centeno-Martinez et al., 2022. Sequencing of the 16S rRNA gene amplicons was performed using the Illumina MiSeq platform (2x250, paired-end).