Hi-C data of M-phase Streptomyces coelicolor A3(2) cells rep1
Hi-C libraries were constructed according to previous studies, Briefly, bacteria cells were cross-linked with 3% formaldehyde for 1 hour at 4 and quenched with 0.375M final concentration glycine for 15 min. The cross-linked cells were incubated with lysozyme at 37C for 10min to lyse the cells. Endogenous nuclease were inactivated with 0.5% SDS, then chromatin DNA were digested by 50U Sau3AI (NEB), and marked with biotin-14-dCTP (Invitrogen) and then ligated by 50U T4 DNA ligase (NEB). After reversing cross-links, the ligated DNA was extracted through QIAamp DNA Mini Kit (Qiagen) according to manufacturers instructions. Purified DNA was sheared to 300- to 500-bp fragments and were further blunt-end repaired, A-tailed and adaptor-added, followed by purification through biotin-streptavidinmediated pull-down and PCR amplification. Finally, the Hi-C libraries were quantified and sequenced on the Illumina Nova-seq platform