Hi-C sequencing data of Arhopalus rusticus
An adult of cut into pieces and mixed with 2 % formaldehyde solution for cross-linking. Glycine (2.5 M) was added to stop this reaction, and the sample was homogenized to separate the nuclei. The purified nuclei were dissolved in SDS and incubated at 65 C for 10 min. After quenching the SDS with Triton X-100, the sample was digested with Dpn II and marked by incubating with biotin-14-dCTP. Biotin from nonligated DNA ends was removed by T4 DNA polymerase.