RNA-Seq of Melanoplus sanguinipes: adult female head
Sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) following the manufacturers recommendations and index codes were added to attribute sequences to each sample. To select cDNA fragments of preferentially 150~200 bp in length, the library fragments were purified with the AMPure XP system (Beckman Coulter, Beverly, USA) and the library quality was assessed on the Agilent Bioanalyzer 2100 system. After that, the library preparations were sequenced on an Illumina NovaSeq 6000 and paired-end reads were generated. The raw reads were firstly processed through fastp for quality control and filtering the quality reads. The clean reads from all the biological replicates and tissue samples were pooled together to assemble de novo transcriptome using Trinity with min_kmer_cov set to 2 and all other parameters were set as default.