Nanopore DNA sequencing of the Ca. Chlorohelix allophototropha enrichment culture: subculture 15.c
Extracted genomic DNA (370 ng) was spiked with 125 ng of Lambda Phage DNA (EXP-CTL001; Oxford Nanopore Technologies). This DNA solution was used as input to the Ligation Sequencing Kit (SQK-LSK110) for library preparation. Long Fragment Buffer was used as part of the kit. The resulting library was sequenced on a R9.4.1 Flongle flow cell while using adaptive sampling to deplete reads that matched the Lambda phage genome. DNA data was basecalled using Guppy 5.0.16 with the Super Accuracy (SUP) model.