small RNA Seq of rcf1-4
Total RNA extracted from 14-day-old seedlings of both wild type and mutants. For library construction, 30g total RNA were resolved on a 15% polyacrylamide gel with 8M urea. Small RNAs in the 15-40 nt size range were recovered as described. Small RNA libraries were prepared using the library preparation kit (NEB, E7100), and sequenced with an Illumina HiSeq2502 platform at BerryGenomics, China.