RRBS-seq of mus musculus:male testis
Genomic DNA was extracted and subjected to RRBS library preparation using the  Rapid RRBS Library Prep Kit  according to the manufacturers protocol. In brief, 100 ng of genomic DNA was digested with MspI, end-repaired, 3'-dA-tailed and ligated to 5-methylcytosine-modied adapters. After bisulte treatment, the DNA was amplied with 12 cycles of PCR using Illumina 8-bp dual index primers. Size selection was performed to obtain DNA fractions of MspI-digested products in the range of 100-350 bp using a dual-SPRI protocol according to the manufacturers protocol. The constructed RRBS libraries were then analyzed by Agilent 2100 Bioanalyzer and finally sequenced on Illumina platforms using a 1507 paired-end sequencing protocol.