16S amplicon-seq of middle ear microbiome in otitis media with effusion in children
ZymoBIOMICS DNA Miniprep Kit was used for the isolation of DNA from the sample. The DNA concentration was measured by a Quibit fluorimeter with Quibit dsDNA HS Assay Kit. After PCR amplification of microbial 16S rRNA gene sequences, NGS was carried out by MiSeq Reagent Kit vs3 (600 cycle) in an Illumina MiSeq System.