RNA-Seq of AC109347.1 overexpressed mus musculus 2
Enrich eukaryotic mRNA using magnetic beads with Oligo (dT), then break the mRNA into short fragments. Using mRNA as a template, synthesize a single stranded cDNA using random hexamers primers, and then synthesize a double stranded cDNA and purify it. The purified double stranded cDNA is first subjected to end repair, followed by the addition of an A tail and the connection of sequencing adapters. Then, AMPure XP beads are used for fragment size selection. Finally, PCR amplification was performed and the PCR products were purified using AMPure XP beads to obtain the final sequencing library.