RNA-seq of Papilio polytes alphenor dsxh male, P0 hindwing, replicate 2
Hindwings were dissected in room temperature PBS, then immediately stored in RNAlater at -80C until RNA extraction using TRIzol. RNA was treated with Dnase, then depleted of rRNA using the RNase H method. Depleted RNA was then converted into libraries using the KAPA RNA HyperPrep Kit and sequenced 1x50 bp on an Illumina HiSeq 4000. Each replicate consisted of one hindwing from each of three individuals from on single male x single female cross. Only individuals homozygous for one or other doublesex allele (mimetic dsxH or non-mimetic dsxh) were used in these experiments.