Cell-free Plasma EV RNA was prepared with Perkin Elmer's NEXTFLEX Small RNA-seq v2 kit and isolated with Qiagen's ExoRNeasy MIDI Kit
Cell-free RNA from  plasma EVs  was library prepared using Perkin Elmer's NEXTFLEX Small RNA-seq v2 kit, with barcoded primers, according to the manufacturer's instructions. Plasma EV RNA was isolated using QIAGEN's ExoRNeasy Midi kit, according to the manufacturer's instructions.