16S rRNA gene sequencing of mus musculus: cecal contents
Cecal content was obtained using sterile equipment under a flow laminar hood immediately after termination and were stored at ?20?C until microbial DNA isolation. Microbial DNA was isolated from frozen cecal contents with a DNA isolation kit according to protocol (Mo Bio Laboratories, Inc., Carlsbad, CA, USA). Amplification of the 16 S small subunit rRNA gene was performed using standard protocols of the Earth Microbiome Project (www.earthmicrobiome.org).  The V4 region of the 16S gene was targeted with universal primers 515 F/806RB and sequenced with an Illumina MiSeq.