18S amplicon metabarcoding of hydrothermal vent plume water: Cast 1, Bottle 3, Filter 1
Metagenomic DNA was extracted with the Macherey-Nagel NucleoMag DNA/RNA Water kit. DNA quantity was measured with the Qubit HS DNA assay. Eukaryote-specific primers used to amplify part of the hypervariable 18S SSU rRNA gene: 5-GCTTGTCTCAAAGATTAAGCC-3 and 5- GCCTGCTGCTGCCTTCCTTGGA-3, forward and reverse respectively (Blaxter et al. 1998). Primers included linker sequences on their 5 ends to additional of Illumina-compatible dual-index adapter sequences. Sequencing was performed on a MiSeq using V3 reagents (2x300 bp).