RNA sequence of DB _Root
Method: Total RNA was isolated from the root and leaf tissues of one-year-old D. binectariferum plants using TRIZOL reagent (Sigma Life Science, USA) (Meng and Feldman, 2010). Each sample included three biological replicates. Samples showing acceptable RNA integrity number above 7 were used in library preparation and sequencing was done from both 5 and 3 ends on the Illumina platform (NovaSeq 6000) according to the manufacturers instructions (Illumina Inc., San Diego, CA, USA)