Input and ChIPseq of total and acetylated Pf H2B.Z in Plasmodium falciparum with loxP flanked bromodomain in PfGCN5 excised by induction of diCre by rapamycin or left intact in dmso control
ChIP DNA samples were quantified with the Qubit dsDNA HS Assay Kit (Q32851) (Invitrogen) and 4 ng of each ChIP DNA were used to make sequencing libraries with the NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645) but using KAPA HiFi PCR kit containing high-fidelity KAPA HiFi DNA polymerase enzyme (Roche) for 12 cycles of library amplification  using the conditions 98 C for 1 min for the initial denaturation followed by 12 cycles of 98 C for 10 s and 65 C for 1 min and a final extension at 65 C for 5 min. Libraries were 150 bp  paired-end sequenced on the Illumina Novaseq 6000 system at the Victorian Clinical Genetics Services (VCGS), Murdochs Children Research Institute (MCRI), Parkville, Victoria, Australia.