PacBio sequence reads of the Meyerozyma guilliermondii ATCC 6260 genome
M. guilliermondii strain ATCC 6260 was grown in YPD medium. Spheroplasts were created by zymolyase treatment then lysed with SDS. The sample was phenol/chloroform extracted and precipitated with isopropanol. Following RNAse and proteinase K treatment, DNA was sheared with a Megaruptor 3 to an average fragment length of 13 kb. The library was constructed with the SMRTBell Express Template Prep kit 3.0 (Pacific Biosciences) and sequenced on one SMRTcell 8M using a PacBio Sequel IIe with the CCS sequencing mode and a 30 h movie time. CCS and demultiplexing analysis was done using SMRTLink V11.1.