Shotgun of mus musculus gut metagenome: Clostridia Consortium
DNA extraction is performed using the QIAamp PowerFecal Pro DNA Kit and Illumina compatible libraries are generated using the QIAseq FX Library Kit. Sequencing runs are performed on the Illumina NextSeq platform in the Functional Genomics Facility at UChicago using the 2150 Paired End reads cassette. Shotgun samples are taxonomically profiled using Kraken2 while deep shotgun sequencing can be assembled into metagenomic contigs using metaSPADES and annotated using prokka upon request. Metagenomic contigs may also be taxonomically identified using Kraken2.