Escherichia coli, genome sequence, man, urine culture
For the preparation of genomic libraries, as a first step, Tagment Genomica DNA was carried out, hands-on for 20 minutes for a total of 40 minutes. Post Tagmentation cleanup: hands-on for 20 minutes, Amplify Tagmented DNA: Hands-on 15 minutes and a total 45 minutes. Clean up Libreries was carried out hands-on 20 minutes for a total of 50 minutes. And finally the pool libraries were madeReagents: RBS, TSB,TWD, EPM, Index Adapters, SPB, etOH, BLT, TB1