ChIPseq input of Plasmodium falciparum with TY1 epitope tagged PfGCN5
Input DNA samples were quantified with the Qubit dsDNA HS Assay Kit (Q32851) (Invitrogen) and 4 ng of each ChIP DNA were used to make sequencing libraries with the NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645) but using KAPA HiFi PCR kit containing high-fidelity KAPA HiFi DNA polymerase enzyme (Roche) for 12 cycles of library amplification  using the conditions 98 C for 1 min for the initial denaturation followed by 12 cycles of 98 C for 10 s and 65 C for 1 min and a final extension at 65 C for 5 min. Libraries were 150 bp  paired-end sequenced on the Illumina Novaseq 6000 system at the Victorian Clinical Genetics Services (VCGS), Murdochs Children Research Institute (MCRI), Parkville, Victoria, Australia.