RNA-Seq of Botrytis cinerea B05.10 growing on PDA plates for 24h (12:12 L:O) plus an extra hour in dark
Total RNA extraction was performed with 10 mg of frozen mycelia ground to powder with TRizol reagent. RNA quantity and quality was accomplish with NanoDrop and a fluorescence-based capillary electrophoresis machine respectively. poly-A mRNA molecules were obtained and libraries were constructed using a TruSeq Stranded RNA Sample preparation kit