2hour NSS experimental replica 1
RNA collected using Ribopure Yeast extraction kit; TagSeq library preparation involves adding unique molecular identifiers (UMIs) to RNA molecules, followed by tagmentation to fragment and attach adapters. This is succeeded by cDNA synthesis, PCR amplification, purification, and quality assessment, ultimately producing a high-quality library for transcriptomics analysis. TagSeq was performed at the The Genomic Sequencing and Analysis Facility, University of Texas, Austin.