WES of Human Osteosarcoma
The genomic DNA sample was randomly fragmented by sonication (Covaris, Massachusetts, USA) to the size of 180-280 bp fragments. DNA fragments with ligated adapter molecules on both ends were selectively enriched in a PCR reaction. Products were purified with AMPure XP system (Beckman Coulter, Beverly, USA) and quantified by using the Agilent high sensitivity DNA assay on the Agilent Bioanalyzer 2100 system.