Soil mulched and non-inoculated replication 3
bacterial DNA was extracted using the DNeasy Power Soil kit (Qiagen, Hilden, Germany), following the manufacturers instructions. With the extracted DNA, paired-end amplicon sequencing of the 16S rRNA gene was then performed using the Illumina MiSeq high-throughput sequencing platform at Molecular Research DNA (MR DNA) (www.mrdnalab.com Shallowater, TX, USA) (accessed April 5, 2022). The primer set used was 515F (5-GTGCCAGCMGCCGCGGTAA-3) and 806R (5-GGACTACHVGGGGGTWTCTAAT-3), which are specific to the V4 region of 16S rRNA