16S rRNA amplicon sequencing of human gut metagenome: IBD gut
A two-step PCR protocol was applied by using broad-specificity universal prokaryotic primers, 341F3 (5-ACTCCTAYGGGRBGCASCAG-3) and 806R5 (5-AGCGTGGACTACNNGGGTATCTAAT-3), which targeted the V3-V4 region.