SequencingRun1_PH0919_DimGFP
A library of single-codon substitutions in the rnc gene was constructed using inverse PCR. The library was transformed into NEB 5-alpha cells. Plasmids were prepped from NEB5alpha colonies that grew overnight on bioassay plates. That plasmid library was transformed into PH0919 cells and colonies that grew overnight diluted on bioassay plates were collected and frozen. Frozen aliquots were diluted and sorted based on GFP-fluorescence and forward scatter until greater than 1,000,000 highly fluorescent events were collected. We plasmid prepped and determined the allele frequencies of the low fluorescent cells, replica 1 sample using deep sequencing.