DuplexSeq of Rat: One dose 40 mg/kg ENU by oral gavage (NIEHS_ENU_liver_28 days_rat 16_sample 3_Inotiv)
DNA was fragmented using sonication to an average size of 300 bp. A unique molecular identifier was attached to both strands of a DNA duplex on both ends. A panel of probes (TwinStrand Rat Mutagenesis Panel (Rat-50 v1.0)) was used to isolate 20 2.4 kb-regions in the genome (a total of 50 kb) by hybrid selection, which were then amplified by PCR.