Long read HiFi WGS of the Trypanosoma cruzi strain Dm25
DNA extraction from T. cruzi epimastigotes was performed using the Gentra Puregene kit (Qiagen) to obtain high molecular weight DNA. The DNA was quantified with the NanoDrop 2000 spectrophotometer (Thermo Scientific, USA). The integrity of the DNA was verified by electrophoresis with a 2% agarose gel (90V for 30 minutes). The DNA was sequenced at the Georgia Bioinformatics Core using Pacific Bioscience (PacBio) HiFi technology.