Amplicon sequencing (16S) of wet tropics soils
Amplicons were purified using magnetic beads and subjected to dual indexing using the Nextera XT Index Kit (Illumina) according to the manufacturers instructions. Indexed amplicons were purified using magnetic beads and then quantified using a PicoGreen dsDNA Quantification Kit (Invitrogen). Equal concentrations of each sample were pooled and sequenced on an Illumina MiSeq using 30% PhiX Control v3 (Illumina) and a MiSeq Reagent Kit v3 (600 cycles, Illumina) according to the manufacturers instructions.