genome pacbio sequencing of Acrossocheilus fasciatus :adult male blood
the qualified and high-quality DNA (main band > 30 kb) was randomly interrupt into 15-18 kb size fragments by the Covaris ultrasonic crusher. After enriching, purificating, repairing, adaptering ligation, and filtering, the well constructed libraries were sequenced on Pacbio Sequel II/Pacbio Sequel IIe platform.