WGS of Influenza A virus H5N1: RNA extracted from inoculum for experimental infection of ferrets with PB2-A271T virus virus in respiratory contact experiment
RNA was isolated from nasal wash samples using a RNeasy Mini Kit and vRNA was converted to cDNA using the M-RTPCR protocol (one-step reverse transcription PCR amplification of full viral genomes was achieved using pooled Uni12/Inf-1 and Uni12/Inf-3 forward primers and Univ.RUni13/Inf-1 reverse primers and SuperScript III Platinum kit). cDNA was processed at the Emory National Primate Research Center (ENPRC) Genomics Core for sequencing on an Illumina NovaSeq 6000 platform. Samples were sequenced as 2 x 100 bp paired reads and demultiplexed prior to delivery.