WGS of Lagria villosa: Accessory gland from 6 females
Genomic DNA was extracted using the Nanobind CBB Big DNA kit (Circulomics, Baltimore, USA) followed by enrichment for high molecular weight DNA using the Short Read Eliminator kit XS (Circulomics). End-DNA repair and library preparation was performed using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, Ipswich, USA) and the Ligation Sequencing Kit V14 (SQK-LSK114; Oxford Nanopore Technologies, Oxford, UK) followed by a clean-up step with AMPure XP beads (Beckman Coulter).