SARS-CoV-2 in Wastewater
Wastewater samples were collected, processed and RNA was extracted. Detection of SARS CoV-2 in RNA was done using RT-qPCR. The copy number was determined and the samples.The sequencing process for the samples involved several steps.Illumina Covid seqRuo kits were used for sample library preparation, including random hexamer annealing, first strand synthesis, and cDNA amplification targeting the entire SARS-CoV-2 genome through multiplex PCR reactions. The amplified products were then processed with Illumina PCR Indexes for tagmentation, adapter ligation, and subsequent enrichment and clean-up steps. Pooled libraries were quantified, normalized, and denatured before undergoing single-end sequencing on the Illumina NextSeq 550 platform.