Faecal microbiome of short-beaked echidna: before fire
DNA from faecal samples was isolated using the Qiagen QIAamp Fast DNA Stool Mini Kit. All samples were PCR amplified and uniquely barcoded, using primers targeting the V4 region of the bacteria 16S ribosomal RNA (rRNA) gene, 515F and 806R. Sequencing was performed on an Illumina Miseq.