Fungal Metagenome of Dhonchi Sample
The DNA was isolated from the given samples and the DNA concentration was estimated using Qubit Fluorimeter (V.3.0). The ITS1-ITS2 Inter-transcribed region was amplified using specific ITS1 Forward primer TCCGTAGGTGAACCTGCGG and ITS2 Reverse primer GCTGCGTTCTTCATCGATGC. The amplifiedproduct was checked on 2% agarose gel and gel purification was done to remove non-specific amplifications.5ng of amplified product was used for library preparation using NEBNext Ultra DNA library preparation kit. The library quantification and quality estimation were done in Agilent 2200 TapeStation. Theprepared library was sequenced in Illumina HiSeq 2500 platform.