Sample_GNC17_NormalF_pituitary
High quality RNA samples (RIN range 7-8) were used to prepare the mRNA libraries by SureSelect Strand Specific RNA Library Preparation kit (Agilent Technologies, Santa Clara, California, U.S.). In particular, poly-A selection and directional mRNA libraries were carried out using 1-2 g of total RNA. Finally, paired-end sequencing (2x75 bases) was performed on the Illumina NextSeq 500 platform (Illumina Inc., San Diego, California, U.S.).