Genomic sequencing of Histoplasma strain HcB14 Cas9-2
Dual-indexed paired-end libraries for whole genome sequencing were prepared with the Nextera DNA Flex Library Prep Kit from Illumina. Individual libraries were barcoded with Nextera DNA CD Indexes (Illumina) and analyzed for average size distribution and concentration on a High Sensitivity DNA Bioanalyzer chip from Agilent Technologies. Fragments of individual libraries had an average size distribution of 500-550 bp and 5 ng of each library were pooled for multiplexed sequencing. All 7 strains were sequenced in a single lane on an Illumina HiSeq 4000 at the UCSF Center for Advanced Technology (UCSF CAT) yielding 101-mer paired-end reads with ~400x coverage of the genome per strain.