DNA-Seq of WT_EtOH-Evo
Illumina sequencing library of WT_EtOH-Evo was prepared from isolated DNA using the NEBNext Ultra II DNA Library Prep Kit for Illumina according to the manufacturers instructions (NEB). The libraries were evaluated by qPCR using the KAPA library quantification kit (Peqlab) and then normalized for sample pooling. Pairedend sequencing with a read length of 2  150 bases was performed in-house on an Illumina MiSeq system.