WGS of Lagria villosa: Accessory gland from 13 females
Genomic DNA from the preserved organs was extracted per individual after removing the fixative and homogenizing the tissue in liquid nitrogen. The MasterPureTM complete DNA and RNA isolation Kit (Epicentre Technologies) was used as indicated by the manufacturer, including an additional incubation step at 37 for 30 min with 4  lysozyme (100 mg mL-1) before protein precipitation. The nucleic acids were re-suspended in Low TE buffer (1:10 dilution of TE) and pooled together. End-DNA repair and library preparation was done using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, Ipswich, USA) and the Ligation Sequencing Kit V14 (SQK-LSK114; Oxford Nanopore Technologies, Oxford, UK) followed by a clean-up step with AMPure XP beads (Beckman Coulter).