RNA-seq of mus musculus tumors
Total mRNA was enriched with magnetic beads with Oligo(dT) and fragmented by fragmentation buffer. Then mRNA was reverse transcribed using random primers. Following purification, cDNA was repaired, tailed with dA and ligated with sequence adaptors, then cDNA size distribution pattern was screened by AMPure XP Beads. Filtered cDNA was amplified by PCR to construct the library. RNA-sequencing (RNA-Seq) was performed by Beijing Expandbio Science&Technology company with Illumina NovaSeq 6000 (Illumina, San Diego, CA, USA) according to manufactorys instructions. Raw data was filtered with Trimmomatic and aligned to the reference genome by STAR.