DNA-seq of Neofabraea malicorticis: Hifi
After examination of the quality of isolated DNA, the library of 15 kb was constructed using a SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences, CA, USA). The construction includes DNA shearing, AMPure PB Bead purification, ssDNA overhangs removing, damage repair, end repair, hairpin adapter ligation, and bead purification of the library. After quality control test, a SMRTbell library was obtained. The library was sequenced using a single 8 M SMAT Cell on the PacBio Sequel II/IIe/ PacBio Revio platform (Pacific Biosciences, CA, USA) (PacBio Sequel II/IIe/ PacBio Revio System).