PacBio sequence reads of the Candida parapsilosis CDC 317 (ATCC MYA-4646) genome
C. parapsilosis strain CDC 317 was grown in YPD medium. Spheroplasts were created by zymolyase treatment then lysed with SDS. The sample was phenol/chloroform extracted and precipitated with isopropanol. Following RNAse and proteinase K treatment, DNA was sheared with a Megaruptor 3 to an average fragment length of 13 kb. The library was constructed with the SMRTBell Express Template Prep kit 3.0 (Pacific Biosciences) and sequenced on one SMRTcell 8M using a PacBio Sequel IIe with the CCS sequencing mode and a 30 h movie time. CCS and demultiplexing analysis was done using SMRTLink V11.1.