RNA-Seq of Human: endometrium in mid-secretary phase of natural menstrual cycle
Single-cell suspensions are loaded into a microfluidic chip using the 10x Genomics Chromium system, where they are partitioned into nanoliter-scale Gel Bead-in-Emulsions (GEMs) with barcoded gel beads, enzymes, and partitioning oil. Inside the GEMs, cell lysis and reverse transcription occur, creating barcoded cDNA. After breaking the GEMs, the cDNA is purified and amplified via PCR. The amplified cDNA is then fragmented, end-repaired, adapter-ligated, and subjected to a second round of PCR. The final library is quality controlled and ready for sequencing on an Illumina platform.