Cell-free EV-depleted Plasma was prepared with Perkin Elmer's NEXTFLEX Small RNA-seq v2 kit and isolated with Thermofisher's mirVana Total Exosome RNA Kit
Cell-free RNA from  EV-depleted plasma was library prepared using Perkin Elmer's NEXTFLEX Small RNA-seq v2 kit, with barcoded primers, according to the manufacturer's instructions. EV-depleted RNA was isolated using Thermofisher's mirVana Total Exosome RNA Kit.