RNA-seq of a cardiac angiosarcoma sample
cDNA libraries were synthesized from fragmented RNA, ligated to sequencing adapters and amplified. Subsequently, coding exon sequences were captured by hybridization to a pool of exonic probes. Before sequencing at 80bp, libraries were sized with Agilent DNA 7500 chips on the Bioanalyzer 2100 and quantified with a fluorometric assay.