8-NT1
To uncover the expression pattern of ZmMADS8he endosperm was harvested at 15 DAP to determine the expression of ZmMADS8 in transgenic family lines. These samples were ground into powder in liquid nitrogen, and total RNA was extracted using TRIzol Reagent (Life Technologies) according to the manufacturers instructions. After genomic DNA was removed using DNase I (TaKaRa Biotech, Dalian, China)