sludge
Joint connection; Use magnetic beads to screen and remove self connected fragments of the connector; Enrichment of library templates using PCR amplification; Recycling PCR products with magnetic beads to obtain the final library. Scan the surface of the reaction plate with a laser and read the types of nucleotides aggregated in the first round of reaction for each template sequence; Chemically cleave the "fluorescent group" and "termination group", restore the 3 'end viscosity, and continue to polymerize the second nucleotide; Collect fluorescence signal results for each round and obtain the sequence of template DNA fragments.