Metabarcoding of Centrocercus urophasianus: adult tissue sample for control library
DNA was extracted from cloaca and esophagal swabs taken from Barn Owls using the Qiagen DNeasy Blood and Tissue kit.DNA was amplified at a locus within the mitochondrial 12S gene. Greater Sage-Grouse (Centrocercus urophasianus) DNA was included as both a negative control to track contamination and a positive control to monitor PCR conditions. Purified PCR products were indexed and pooled to allow for multiplexing. Pooled libraries were sequenced on an Illumina Miseq using the 300 Micro V2 kit to generate paired-end 150 bp reads (University of Montana Genomics Core).