spatially-resolved RNA-Seq of homo sapiens: adult female high grade serous carcinoma tumor
A fresh-frozen tumor sample was embedded in Optimal Cutting Temperature (OCT) compound, cryo-sectioned at 10 μm thickness, placed onto Visium glass slides, and stored at -80°C before processing. Tissue optimization was performed following 10X Genomics Visium Spatial for Fresh Frozen Tissue Optimization protocol (User Guide, CG000238). Spatial gene expression libraries were generated following 10X Genomics Visium Spatial Gene Expression for Fresh Frozen protocol (User Guide, CG000239). Libraries were sequenced on an Illumina NovaSeq 6000 instrument.