the muscles of the thoracic or foot regions
The construction of next-generation sequencing (NGS) libraries with an insert size of 150 bp involved several key steps: end repair, A-tailing, the addition of a sequencing junction, purification, PCR amplification, among others. These libraries were then subjected to high-throughput sequencing using the DNBSEQ-T7 platform (Illumina, San Diego, CA, USA)