GSM7610858: AM3; Homo sapiens; RNA-Seq
Tissues were homogenized using pre-chilled Omni Bead Ruptor 24 Cryo Cooling Unit in 2.8mm ceramic tubes and pre-chilled 700 µl QIAzol Lysis reagent. Total RNA was extracted using the Qiagen RNAeasy PLUS Universal Mini Kit per the manufacturer's instructions. Total RNA samples about 500ng were used for sequencing library preparation according to Illumina TruSeq Stradned mRNA Sample Preparation Guide (Illumina Cat. #: RS-122-2201). Each library was bar-coded and then pooled for cluster generation and sequencing run with 50bp single-end (SE) sequencing protocol.