GSM8403242: 4T1, non-adherent, 27HC sEVs treated, 48h, rep1; Mus musculus; RNA-Seq
Total RNA was extracted using Rneasy Plus Mini Kit (Qiagen), according to the manufacturer's protocols. The total RNAs were converted into individually barcoded polyadenylated mRNAseq libraries with the Kapa HyperPrep mRNA kit (Roche). Libraries were barcoded with Unique Dual Indexes (UDI's) which have been developed to prevent index switching. The adaptor-ligated double-stranded cDNAs were amplified by PCR for 10 cycles with the Kapa HiFi polymerase (Roche).