GSM6475319: TMD8-veh-2; Homo sapiens; RNA-Seq
Xenograft tumors from TMD8 and LY6934 mouse models after dosing with AZD5153 and acalabrutinib were excised and snap frozen in liquid nitrogen. Tumor tissue was lysed and RNA was extracted using RNeasy kit (Qiagen, Hildren, Germany) according to manufacturer's instructions. RNA concentration was quantified using the Qubit fluorometer (ThermoFisher, Waltham, MA). 15 RNA samples were sent to Novogene for library preparation and human RNA sequencing. Illumina mRNA TruSeq library was used for library preparation at Novogene. RNA-seq was sequenced on 9 lanes of Illumina HiSeq4000 with paired end 150bp reads by Novogene.