GSM7991648: type2_inh mixed sample (non-demultiplexed); Mus musculus; RNA-Seq
15 days post inoculation and 24 hours post S.Tm challenge, mice were sacrificed with CO2, and spleens were isolated whole. Extracted spleens were mashed on 70um screens with 5mL FACs buffer (1% FBS, 5uM EDTA). Cells were pelleted, treated with RBS lysis buffer, and washed 2x with FACs buffer before lysing pellet directly with 350uL RLT buffer. Libraries assembled according to the Mars-seq2.0 protocol.