Whole-genome sequence of Escherichia coli M15-4
Genomic DNA (gDNA) was extracted from a single bacterial colony cultured in LB broth with the ZR Fungal/Bacterial DNA Miniprep kit (Zymo Research). A 200-bp genomic library was prepared from 1 g gDNA using the Ion Plus Fragment Library Kit (Life Technologies). Briefly, gDNA was mechanical fragmented to a size range of 50-500 bp (mean 200 bp). Then, fragmented DNA was end-repaired, ligated to adapter/barcode sequences, and size-selected to 330 bp. Subsequently, the genomic library was clonally amplified and enriched. The sequencing was performed using the Ion Torrent PGM platform (Life Technologies) with 200-bp chemistry on a 316 chip.