GSM7991643: inhibitors experiment - mixed sample (non-demultiplexed); Mus musculus; RNA-Seq
14 days post inoculation, mice were sacrificed with CO2, and spleens were isolated whole. Extracted spleens were mashed on 70um screens with 5mL FACs buffer (1% FBS, 5uM EDTA). Cells were pelleted, treated with RBS lysis buffer, and washed 2x with FACs buffer before lysing pellet directly with 350uL RLT buffer. Libraries assembled according to the Mars-seq2.0 protocol.