Soil microbes from rice paddy with conventional practice in Phichit Province, Thailand
Metagenomic sequencing of microbial communities from rice paddy soil collected from either conventional or organic farming practices in Phichit Province, Thailand. The gDNA was extracted using Qiagen DNeasy Powersoil kit, followed by PCR using primer pairs targeting the V3 and V4 regions, including overhang adapter sequences. Amplicons were confirmed by Agilent 2100 Bioanalyzer system. MiSeq Illumina Sequencer was employed using paired 300bp reads and MiSeq v3 reagents. The library preparation, quality control, and sequencing was performed by the Omic Sciences and Bioinformatic Center, Faculty of Science, Chulalongkorn University, Thailand.