GSM8422417: N2.4; Caenorhabditis elegans; RNA-Seq
Embryos were harvested with standard bleaching procedure, flash-frozen in liquid N2, and kept at -80C until RNA extraction. Embryo pellets were Dounce-homogenized and RNA was extracted using phenol(Trizol)/chloroform, treated with Turbo DNase, and pure RNA was extracted using chloroform/isopropanol and dissolved in water. RNA with RIN >8 was used for library preparation. Four independent biological replicates (denoted .2, .3, .4, .5) were used. RNA-seq libraries were prepared using the TruSeq Stranded Total RNA Library Prep Gold (Illumina Cat#20020599) following manufacturer's protocol.