GSM7855060: seedling_Col_D_input_2; Arabidopsis thaliana; OTHER
Total RNA was isolated using Direct-zol RNA Miniprep Kits (Zymo, Cat. # R2052). Polyadenylated RNA was purified from total RNA with two rounds of polyA-tail purification using the Dynabeads® mRNA DIRECT™ kit (Thermo Scientific, Cat. # 61012). All the libraries were constructed by the m6A-Sac-seq protocol{Ge, 2023 #34}. The resulting library was purified by AMPure XP beads (Beckman Coulter) following the manufacturer's directions and subjected to next-generation sequencing.