GSM8252545: E0771 Foxm1 knockout, sgRNA #3; Mus musculus; RNA-Seq
E0771 Control, Foxm1 KO sg1, and Foxm1 KO sg3 tumors were harvested and digested with RPMI media containing 2% FBS, 1mg/ml Collagenase IV(Worthington, Cat #LS004188), and 20μg/ml DNAse I(Sigma, Cat #4536282001) at 37 degrees for 1 hr with shaking. Following tissue dissection, single cells were filtered using 40μm filter to remove residual clumps. 10 million cells were taken and dead cells were removed using the dead cell removal kit (Miltenyi Biotec; #130-090-101). Cells were then counted and loaded onto the 10X Chromium controller, targeting 10,000 cells for capture per well. Single-cell RNA sequencing  libraries were generated using the Chromium Single Cell 3' Library & Gel Bead Kit V3.1 kit and 716 Chromium Single Cell 3' Chips according to the manufacturer's instructions (10X Genomics, 717 Pleasanton, CA). 10X Genomics Chromium X