Bos indicus RCC_BLRI_419M 2019-10-19 Adult Male Tissue
Ear tissue sample of adult male of Red Chittagong (Bos indicus) cattle was collected from Bangladesh Livestock Research Institute, Savar, Dhaka, Bangladesh (Longitude / Latitude-23.847 N, 90.257 E) and were transferred immediately to the Animal Genomics and Breeding Laboratory, Bangladesh Agricultural University, Mymensingh, for further analysis during the period of December 2019. The tissue samples were stored in 96% ethanol. Later on, genomic DNAs were extracted and purified using the DNeasy Blood and Tissue Kit (Qiagen, Germany). The concentration and purity of the extracted samples were measured using Nanodrop Spectrophotometer (Model: ND 2000). Purified DNA was sent for library preparation and been sequenced through commercial sequence providers. DNA was sequenced using Illumina NovaSeq platform from Macrogen, Inc. (South Korea). All the methods had been performed in accordance with the approved regulations for Animal Experimentation in Bangladesh Agricultural University Research System (BAURES).