GSM7991631: P2_RP_pos; Mus musculus; RNA-Seq
14 days post inoculation, mice were sacrificed with CO2, and spleens were isolated whole. Extracted spleens were mashed on 70um screens with 5mL FACs buffer (1% FBS, 5uM EDTA). Cells were pelleted, treated with RBS lysis buffer, and washed 2x with FACs buffer before proceeding to sorting. Using a FACs Aria III, cells were bulk sorted (~8K cells/tube) based on expression of Ly6C, F4/80, and TdTomato. RNA-seq libraries were prepared according to Cel-seq libraries protocol (Hashimshony, T. et al Genome Biology 2016)