GSM7993380: C57BL/6J_day17_sample1; Mus musculus; RNA-Seq
Total RNA were isolated from the dissected fetal brain samples using an AllPrep DNA/RNA Mini Kit (Qiagen, Cat No./ID: 80204) following the manufacturer's instruction. A total of 27 samples were used which included 3 strains x 3 time points x 3 replicates. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection.