GSM8198270: JSH9_E14 replicate 1; Mus musculus; OTHER
Isolation of gDNA samples was performed as described in Pfaffeneder, T. et al., Nature Chemical Biology 10, 574–81 (2014) with the following modifications: Cultures were washed in PBS and lysed by adding RLT buffer (Qiagen) containing 1:100 β-mercaptoethanol. Isolation of genomic DNA was performed with Zymo-Spin IIC-XL columns according to the instruction of the ZR-Duet DNA/RNA MiniPrep Kit (Zymo Research). Lysates were applied onto spin columns and bound material was incubated for 10 min with Genomic Lysis Buffer (Zymo Research) supplemented with 0.2 mg/mL RNaseA (Qiagen). After washing genomic DNA was eluted in water.