RNAseq of E. coli
For RNA-Seq analysis, libraries were constructed following the manufacturer's protocols, employing the NEBNext rRNA Depletion Kit (Bacteria) (New England BioLabs) in combination with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina (Unique Dual Index Primer Pairs) (New England BioLabs).