Acly HIC1
To anchor hybrid scaffolds onto the chromosome, genomic DNA was extracted for the Hi-C library from XXX. Then, we constructed the Hi-C library and obtained sequencing data via the Illumina Novaseq/MGI-2000 platform. In brief, freshly harvested leaves were cut into 2 cm pieces and vacuum infiltrated in nuclei isolation buffer supplemented with 2% formaldehyde. Crosslinking was stopped by adding glycine and additional vacuum infiltration. Fixed tissue was then grounded to powder before re-suspending in nuclei isolation buffer to obtain a suspension of nuclei. The purified nuclei were digested with 100 units of DpnII and marked by incubating with biotin-14-dATP. Biotin-14-dATP from non-ligated DNA ends was removed owing to the exonuclease activity of T4 DNA polymerase. The ligated DNA was sheared into 300600 bp fragments, and then was blunt-end repaired and Atailed, followed by purification through biotin-streptavidin-mediated pull down. Finally, the Hi-C libraries were quantified and sequenced using the Illumina Novaseq/MGI-2000 platform.