RNAseq of Babraham pig peripheral blood mononuclear cells
PBMCs, processed in triplicate, were isolated from heparinized whole blood using Histopaque-1083. Cells were disrupted in RLT Buffer containing beta-mercaptoethanol and homogenized using QIAshredder spin columns (Qiagen, GmbH). RNA was extracted and purified using the Qiagen RNeasy Plus Mini Kit which includes a gDNA removal step. Libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina.