GSM7610844: RNAseq_NT2_shBrat1_Day28_noDox_rep1; Homo sapiens; RNA-Seq
500ng of the Total RNA was extracted using Trizol reagent (Thermo Fisher Scientific, #15596026) according to the manufacturer's instructions. Genomic DNA was removed by Turbo DNAse treatment (Invitrogen, #AM1907). The quality of total RNA was assessed by running it on 1% agarose gel and visualized by ethidium bromide staining. Total RNA-seq libraries were depleted from ribosomal RNA and produced using Stranded Total RNA library prep kit (Illumina, #20020596). RNA samples were treated by Turbo DNase RNase free Kit (Invitrogen, #AM1907) to remove genomic DNA.