GSM7104893: Ovarian cancer, NACT, Post (R200674); Homo sapiens; RNA-Seq
Total RNA from FFPE tissues was extracted using 2 to 5 10-μm FFPE sections from pre- and post-treatment samples with FormaPure RNA kit (Beckman Coulter). RNA concentration and absorbances were analyzed with NanoDrop spectrophotometer (Thermo Fisher Scientific), and quality profiles assessed on Tapestation (Agilent Technologies). Libraries were prepared with the TruSeq RNA Exome kit (Illumina)