scRNAseq of niPSC-OS1 cells in PXGL
For 10x Genomics Chromium Single Cell sequencing, samples were multiplexed using 3' CellPlex Kit Set A. Single cell capture and library preparation was performed on a Chromium Controller using the Chromium GEM Single Cell 3 Kit version 3.1 as per the user guide, targeting a recovery of 3,000 cells per sample. We targeted a gene expression (GEX) coverage of 40,000-50,000 reads per cell, and 10,000-12,0000 reads per cell multiplex oligo. Sequencing was performed on a Novaseq 6000 instrument generating paired end reads (28x10x10x90 bases). Sample demultiplexing, alignment, and quantification of barcode counts was performed using CellRanger Multi.