Hyposoter didymator gDNA of dsGFP injected calyx cells at pupal stage 3 -replicate 2
gDNA was extracted using phenol-chloroform method. Calyx samples were incubated in proteinase K (Ambion, 0.5 ug/ul) and Sarkosyl detergent (Sigma, 20%), then treated by RNAse (Promega, 0.3 ug/ul). Total gDNA was extracted by phenol-chloroform and ethanol precipitation. Following extraction, gDNA was quantified using a QBIT fluorometer, then sent for sequencing to Genewiz/Azenta company.