GSM6806569: WT1; Bdellovibrio bacteriovorus; RNA-Seq
RNEasy kit (Qiagen), including DNase I treatment. RNA quality was assessed using an Agilent 2100 Bioanalyser. For the B. bacteriovorus samples (all RIN scores > 9.5), ribosomal RNA was depleted using the NEBNext®rRNA Depletion Kit (Bacteria), and NGS libraries made using the NEBNext® Ultra™ II Directional RNALibrary Prep Kit for Illumina® according to manufacturer's instructions. Paired-end 55bp reads were generated on a NextSeq 2000 with dual 8bp indexing. For the A. aeolicus samples (all RIN scores > 5), rRNA was depleted using Illumina Ribozero Kit (Bacteria), and NGS libraries made using the TruSeq Stranded Total RNA LT Kit according to manufacturer's instructions. Single End 50bp reads were generated on a MiSeq with single 6bp indexing.