GSM7914735: Adult Urothelium Replicate 4; Mus musculus; RNA-Seq
Cells were sorted directly into the Takara SMART-Seq HT lysis buffer containing RNase Inhibitor + 3' SMART-Seq CDS Primer II A (SMART-Seq® HT Kit, Takara Bio USA, Ann Arbor, MI). Samples were immediately frozen on dry ice, stored at -80ºC, then transported to The Ohio State University Comprehensive Cancer Center NextGen Sequencing Shared Resource facility. lcRNAseq libraries were generated using the Takara SMART-Seq HT Kit, Nextera XT DNA Library Preparation and Nextera XT Index kits (Illumina, Inc., San Diego, CA). Samples were evaluated using a High Sensitivity DNA Bioanalyzer and Tapestation HS-DNA1000 Bioanalyzer (Agilent), and Qubit (ThermoFisher). Sequencing was performed using a NovaSeq6000 SP 200 cycle (≤2x100bp) flow cell.