GSM7032099: P14_Mut Astrocytes  [MUT2-AST_S10]; Mus musculus; RNA-Seq
RNA was extracted from astrocytes by adding 300 µl of Trizol (ThermoFisher) to the cell pellet, then RNA was purified using the Direct-zol RNA Microprep (R2060, ZYMO RESAERCH), according to manufactures protocol, and eluted in 15 µl of DNase/RNase-free water RNA-Seq Libraries were generated using the TruSeq strand mRNA kit (Illumina). The RNA-Seq libraries were quantified by Qubit and qPCR according to the Illumina Sequencing Library qPCR Quantification Guide and the quality of the libraries was evaluated on Agilent Bioanalyzer 2100 using the Agilent DNA-1000 chip. The RNA-Seq library sequencing was performed using Illumina NextSeq 500.