RNA-seq of sus scrofa
Using the NEBNext Ultra II RNA Library Prep Kit for Illumina  and mRNA with polyA tails was enriched by Oligo(dT) magnetic beads, final, confluent RNA into cDNA. "Double-stranded cDNA was purified, and PCR amplified and used again, AMPure XP beads were used to purify the PCR products and finally obtain the library.