=_xlfn.CONCAT("mrna sequencing of zebrafish: ",A5)
NGS library prep was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following Illuminas standard protocol (Part # 15031047 Rev, E). Libraries were prepared with a starting amount of 193 ng and amplified in 13 PCR cycles, Libraries were profiled in a DNA 1000 Chip on a 2100 Bioanalyzer (Agilent technologies) and quantified using the Qubit dsDNA HS Assay Kit, in a Qubit 2,0 Fluorometer (Life technologies). All 16 samples were pooled in equimolar ratio and sequenced on 1 NextSeq 500 Highoutput FC, SR for 1 x 84 cycles plus 7 cycles for the index read.