bulk RNA-seq of pre-stimulated ND451 PRDM1 KO CAR-T
On days 0, 5 and 20 of the CAR T-cell restimulation assay, before stimulation and after the fourth tumor challenge respectively, CD8+ CAR T-cells were isolated using CD8 microbeads (Miltenyi Biotec) and total mRNA was extracted with RNA Clean & Concentrator kits (Zymo Research). Bulk RNA-seq was conducted by Novogene using the Novaseq6000 system (paired-end 150bp) at 40  106 reads per sample.