Nucleases activity comparison in C2C12: AAV2 delivered Un1Cas12f1 targeting Rosa26, replicate 3
C2C12 cells were seeded at 5x10^4 gc per well on 48-well plate. AAV2 was added to cells at a multiplicity of infection of 10^4 gc per well. Cells were collected 4 days after transduction for genomic DNA extraction and editing efficiency analysis.