GSM7434916: Suspension Cultured Pancreas Exocrine - Cryo Hu958 D7; Homo sapiens; RNA-Seq
Single cells were captured on a 10xGenomics Chromium Controller using a 10X V3 Single Cell 3' Solution kit (10xGenomics, Chromium Single Cell 3' Reagent kit V3, Cat. PN-1000092). All protocols were performed following the manufacturer's instructions. Final sequencing libraries were analyzed on a High Sensitivity DNA Chip (Agilent, Cat. 5067-4626). The library concentration was determined with a Qubit High Sensitivity DNA Assay Kit (Thermo, Cat. Q32854). The libraries were sequenced with the paired-end setting of 28 cycles of read (R) 1, 101 cycles of R2, and 8 cycles of index reads on Illumina NovaSeq 6000 platform, with sequencing depth of ~0.1 million reads per cell.