GSM7993437: P90_ATAC_heaEC_P30Tam_Sox9_OE_r3; Mus musculus; ATAC-seq
cardiac EC were isolated based on MACS-magnetic MicroBeads technology from Miltenyi Biotec and FACS sorted for CD146 for a 80-90% purity. Isolated cardiac EC were used for ATACseq was performed using standard protocols with minor modifications. Briefly, nuclei were isolated from the cells of interest and subjected to transposition with sequencing adapters. The transposed DNA was purified and amplified by PCR, and the resulting library was sequenced at 100bp paired end.