GSM6596606: seu-6, 1/2 MS+250mM Sorbitol, 6h, 10-day old whole seedlings,rep2; Arabidopsis thaliana; RNA-Seq
Total RNAs was extracted with the Trizol reagent. After DNA digestion, poly(A)+ RNAs were purified using Dynabeads mRNA Purification Kit (Invitrogen, USA). RNA-seq libraries were constructed using NEXTflex RNA-Seq Kit (Bioo Scientific, USA). Briefly, RNAs were fragmented and the first strands and second strands of cDNAs were synthesized. After the addition of a single nucleotide “A”, cDNAs were subjected to adapter ligation. The products were purified with AMPure XP beads and then used as templates for PCR amplification.