HiC_WGS_Pyrocephalus_nanus_IslaSantaCruz
Muscle tissues from the same sample were homogenized using a sterile razor blade on ice. An in-situ Hi-C library was prepared as described in (Rao et al. 2014) with a few modifications. Briefly, after the Streptavidin Pull-down step, the biotinylated Hi-C products underwent end repair, ligation and enrichment steps using the NEBNext UltraII DNA Library Preparation kit (New England Biolabs Inc, Ipswich, MA). Titration of the number of PCR cycles was performed as described in Belton (Belton et al. 2012).