GSM7437346: NT  1dpc  rep2; Mus musculus; RNA-Seq
Retinas were dissected in Ames solution, digested in papain, and dissociated into single cells. The cells were then stained with anti-CD90.2 and Calcein Blue (a live cell marker), and Calcein Blue+ RGCs were collected based on CD90.2 and/or GFP (under the Vglut2 promoter) and/or mCherry (co-expressed with sgRNAs) using FACS. RNA from FACS-sorted RGCs was extracted using the RNeasy Micro Kit (Qiagen). RNA-seq libraries were prepared with SMART-Seq v4Ultra Low Input RNA Kit. Libraries were sequenced using a HiSeq4000 instrument to generate 69bp pair-end reads.