GSM6805844: MPC-wholetumor; Mus musculus; RNA-Seq
For whole tumor samples, samples from PT, LM and PC were processed for RNA isolation, library preparation, single cell RNA sequencing and count data generation by Singleron Biotechnologies via established protocols (Cologne, Germany). Tissue from 6 mice per location were pooled before. Single cell capture, reverse transcription, and library preparation were carried out on the Chromium platform (10X Genomics) with the single-cell 3' reagent v2 protocol according to the manufacturer's recommendations using 50,000 cells as input per reaction well. The final libraries [PT, LM, PC] were pooled and sequenced on two Illumina NovaSeq SP lanes (paired-end 26 bp + 96 bp).