GSM8289721: DMSO_3_MeRIP; Homo sapiens; RIP-Seq
Total RNA was isolated and purified with TRIzol reagent (Invitrogen, USA) following the manufacturer's procedure. Poly (A) RNA was extracted using Dynabeads Oligo (dT)25-61005 (Thermo Fisher, USA). Then Magnesium RNA Fragmentation Module (NEB, cat. e6150, USA) was used to randomly fragment RNA and m6A-specific antibody (Synaptic Systems, cat.202003, Germany) was applied for immunoprecipitation (IP). Then input and IP RNA samples were subjected into next-generation sequencing on an illumina Novaseq™ 6000.