ONPs exposure experiment WGBS data of F0N sample replicate 1
Before sequencing, we extracted DNA from 30 adult female P. nana in each experimental groups with 3 replicates. Whole-bodies of P. nana were homogenized in five volumes of genomic DNA extraction buffer (100 mM NaCl; 10 mM Tris-Cl, pH 8.0; 25 mM ethylenediaminetetraacetic acid [EDTA]; 0.5% sodium dodecyl sulphate; 100 g/mL proteinase K; and 1 g/mL RNase) using a Teflon homogenizer and incubated overnight. We followed the method using phenol and chloroform, and isopropanol to extract DNA and precipitated it with ammonium acetate (a final concentration of 0.2 M) at 10,000g for 10 min. The pellet was rinsed with 70% ethanol, dried, and re-suspended in TE buffer (10 mM TrisCl, 1 mM EDTA, pH 8.0). The extracted DNA then measured their quantity and quality using a Qiaxpert and visualized by 1% agarose gel electrophoresis. The extracted DNA were then conversed by sodium bisulfite treatment and sequencing libraries were prepared using WGBS library kit. The conversed libraries were sequenced using Illumina Novaseq 6000 instrument (Illumina, San Diego, CA, USA).