GSM7993190: Human embryonic stem cells (WA09) at Day 6 of vagal neural crest differentation; Homo sapiens; RNA-Seq
Cells were dissociated into single cells at specific time points using TrypLE (5min at 37oC). Cells were resuspended in DMEM:F12 and centrifuged to remove dissociation reagent. To crypreserve, cells were resuspended in STEMCELL-BANKER and stored at -80oC. Cells were shipped to Single Cell discoveries for scRNAsequencing analysis using 10x genomics Library preparation was carried out using the 10x Genomics 3' v3.1 kit followed by sequencing on an Illumina Novaseq 6000.