illumina DNA sequencing of Zacco platypus
Muscle tissues were excised from these frozen specimens for the extraction of high molecular weight (HMW) DNA using a conventional CTAB-based method. The genomic DNA library was prepared following the protocol of the Illumina TruSeq Nano DNA Library preparation kit (Illumina Inc.). Highthroughput sequencing was performed using an Illumina NovaSeq 6000 platform (Illumina Inc.) following provided protocols for 2 x 150 pairedend sequencing.