RNA-Seq of black clam: Villorita cyprinoides
Total RNA was isolated from five distinct tissues collected from individuals (mantle, gills, adductor muscle, foot and gonads), using 50 mg of each tissue type. After determining RNA concentration, the five RNA extractions were pooled in equimolar concentration. The quality of the RNA pool was assessed on an Agilent Bioanalyzer before normalized random primed cDNA library preparation.