GSM7773254: MSU 24h rep2; Mus musculus; RNA-Seq
At 8 h or 24 h after injection, mice were deeply anesthetized and were perfused transcardially with 20 mL 0.9% saline (4℃). After the perfusion, the right ankle joints were collected and were immediately preserved in the RNA later solution (Thermo Fisher, USA). Total RNA was extracted using the Trizol reagent (Thermo Fisher, USA) following the manufacturer's instructions.  DNase I was used to degrade contaminating DNA. The purity and concentration of the samples was assessed by Nanodrop Spectrophotometer (NanoDrop Products, USA) and the RNA integrity was assessed by Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA). RNA libraries were prepared for sequencing using standard BGI-SEQ500 protocols