GSM7951674: LT-HSCs_Ikba_WT_1; Mus musculus; RNA-Seq
Direct RNA isolation with RNeasy Mini Kit [Qiagen Ref. 74004] Samples were processed to obtain cDNA following manufacture's protocol. cDNA amplification was performed by Long Distance PCR (LD-PCR) and the PCR-amplified cDNA purified byimmobilization on AMPure XP beads (Agencourt AMPure XP kit). Samples were analyzed with Agilent High Sensitivity DNA Kit (Agilent, Cat. No. 5067-4626). Covaris shearing was used for Illumina Low input sample preparation andDouble last bead purification was performed to remove fragments below 200 bp.Next, samples wereused to generate an Illumina sequencing library byNEBNext Ultra protocol following kit instructions. After PCR amplification of the library, the quality was checked on a Bioanalyzer andtotal cDNA was sequenced using an Illumina HiSeq 2000 sequencer. RNA-seq single-end 50bp