GSM6806737: retina, 40 hr apf, w[*] sev[14], rep 3; Drosophila melanogaster; RNA-Seq
RNAlater was removed from samples prior to homogenization with a plastic pestle. Total RNA was isolated using the PureLink RNA purification kit (Invitrogen, 12183020) according to the manufacturer's instructions along with on-column PureLink DNase treatment (Invitrogen, 12185010). Library construction (from polyA-selected RNA) and sequencing was performed by the Genomic Sequencing and Analysis Facility at UT Austin, Center for Biomedical Research Support (RRID# SCR_021713) using the Illumnia MiSeq  v3 Reagent Kit (paired-end, 150 cycles; MS-102-3001) and platform.