GSM7033975: D-lactic acid-producing Pichia pastoris with ScFLO1 overexpression, replicate 3; Komagataella pastoris; RNA-Seq
The pellets were washed with 5 mL of distilled water. Total RNA was extracted using the QIAgen RNeasy Kit following the manufacturer's protocol. Contaminating genomic DNA was removed from the RNA samples by DNaseI (NEB) digestion using the manufacturer's protocol. The RNA quantity was analyzed using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies), and samples were stored at -80 °C until transcriptomics analysis. RNA Integrity Numbers (RIN) for the purified RNA samples were obtained using an Agilent 2100 Bioanalyzer (Agilent Technologies). All purified RNA samples had a RIN number higher than 8.0 and were sequenced by NovogeneAIT (Tianjin, China). Paired-end 150bp