GSM8326509: RNA-seq, IFNAR KO, DMXAA treated, rep 2; Mus musculus; RNA-Seq
Total RNA was isolated with the Omega Biotek Total RNA II kit, and DNAse-treated with TURBO DNAse (ThermoFisher Scientific, AM2238) according to manufacturer instructions. RNA was polyA selected, and libraries were prepared with  KAPA HyperPrep reagents (Roche) after heat fragmentation. Libraries were subsequently size selected between 450-500 bp.