Illumina 16S amplicon sequencing of EC3T7
gDNA isolation from larvae tissue was performed following the protocol of Qlamp PowerFecal Pro DNA Kit (Qiagen). Total gDNA was purified using CleanNA CleanNGS purification beads (Labgene) and resuspended in Tris-EDTA buffer (Sigma-Aldrich), pH 8.0. Quality and purity of the gDNA isolations were assessed by Nanodrop and Qubit (dsDNA High-Sensitivity kit (HS)). For library preparation, the 16S rRNA V4 region was subjected to Nextera two-steps PCR amplification using the 5A5F primer (GTGYCAGCMGCCGCGGTAA), and 806R primer (GGACTACNVGGGTWTCTAAT) by Microsynth AG. Sequencing on Illumina MiSeq, v2, 2x250 bp. Sequencing data were generated as adaptor trimmed, demultiplexed.