GSM8197901: wo. MMF, 817, HFbD; Mus musculus; RNA-Seq
Treated neutrophils (3 - 5 x 106 cells per sample) were then pelleted and resuspended in TRIzol (1 ml, Invitrogen) before total RNA extraction via RNeasy Plus Universal Mini Kit (Qiagen) following manufacturer's protocol. Libraries were generated using total RNA (5 µl per sample) and QuantSeq 3' mRNA-Seq V2 Library Prep Kit FWD with UDI (Lexogen) following manufacturer's instructions.