GSM8252838: EWS502_RNA_dNEG_1uM_24h_REP2_230830_ACTTGA_S8; Homo sapiens; RNA-Seq
For RNA isolation, cells were lysed in 350 µl of RLT buffer and RNA was isolated using the RNeasy Mini Kit (Qiagen) according to manufacturers instructions. rRNA depletion and library preparation were performed using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA). Illumina Next Gen Sequencing NextSeq platform (Illumina, San Diego, CA) was used to obtain unique sequencing paired-end reads