sRNA-seq of R. pedestris gut
all products were reverse transcribed by PCR amplification. Of these products, the 140160 bp PCR segments were purified and enriched to generate cDNA libraries, whose quality was estimated on an Agilent ioanalyzer 2100 system by DNA High Sensitivity Chips. Finally, the small RNA libraries were sequenced using the DNBSEQ platform at Beijing Genomics institution (Wuhan, China) Technology Co., Ltd.