16S rRNA gene (V3-V4) agricultural drainage ditch sediments: a mesocom experiment
Fisher BioReagents SurePrep Soil DNA Isolation Kit; then OneStep PCR Inhibitor Removal Columns (Zymo Research Corportation);final DNA concentration of 5 ng uL-1 in 10mM tris-HCl (pH 8.5); 600 bp (2 X 300) paired-end sequencing run: Forward Primer (5'- TCGTCGGC- AGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG-3) and Reverse Primer (5'-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTA- TCTAATCC-3).