Illumina MiSeq paired end sequencing; Transcriptome- and fluxome-guided metabolic engineering of 3-hydroxypropionic acid production in Saccharomyces cerevisiae
Transcriptome- and fluxome-guided metabolic engineering of 3-hydroxypropionic acid production in Saccharomyces cerevisiae
The reference REF strain, and strain ST938 were grown in 1 L continuous cultivation bioreactors. After three residence times, biomass samples were harvested as previously described in (Kildegaard et al., 2016. PMID: 26980206). RNA extraction was performed as described in Kildegaard et al., 2014 (PMID: 25263954) Sequencing libraries were prepared as described in (Kildegaard et al., 2016. PMID: 26980206).