GSM8197213: ATAC,Zf5,rep1; Drosophila melanogaster; ATAC-seq
Embryos were staged at NC12-NC13; placed on double-sided tape and rinsed before they were placed in 1.5 mL tube caps. Embryos were macerated with an 18-gauge needle in lysis buffer containing 10mM Tris-HCl pH7.5; 10mM NaCl, 3mM MgCl2; 0.1% Igepal-630. ATAC libraries were prepared using Illumina Tagment DNA Enzyme and Buffer Small Kit with Nextera DNA CD indexes following manuafactor's protocol and previously described methods from Blythe, SA and Buenrostro JD.