GSM7033718: MEF 4in1_EnR_OE day07 rep2; Mus musculus; RNA-Seq
Cells were harvested at day 7 by the addition of TRIzol to the cultures (Invitrogen). RNA harvested in TRIzol (Invitrogen) was isolated using the RNA Miniprep kit (Zymo Research). For bulk RNA sequencing, libraries were prepared according to the dUTP protocol and paired-end sequencing (2x100bp) was performed on the NovaSeq 6000 platform (Illumina).