GSM8197029: ATAC Individual 5 skin fibroblast cells, Dexamethasone, Time36; Homo sapiens; ATAC-seq
For the collection of cells for the ATAC protocol, cells were dissociated using 500uL of prewarmed TrypLE (ThermoFisher 12604013) and left for 5 min at 37°C. TrypLE was inactivated using 500uL of DMEM. Cells were then counted using the Logos Biosystems LUNA-FL automated cell counter, and 50x10^4 cells were used as input for tagmentation. ATAC seq libraries were generated following the previously described protocol (Buenrostro et al. 2015) and sequenced with 75-base double end reads, and sequenced at a targeted depth of 61M reads per sample.