GSM7992567: Old_rep 1; Mus musculus; RNA-Seq
Epithelial cell suspensions were isolated from the dorsal skin of C57BL/6N mice. Subcutaneous fat was removed with a scalpel, and then the whole skin was placed dermis down in 0.25% trypsin-EDTA for 45min at 37℃ to separate the epidermis from the dermis. Single epidermal cell suspensions were obtained by gently scraping the epidermis and then filtering the cells through a cell strainer (40 µM). Dead cells were removed using the MACS Dead Cell Removal Kit. Single-cell cDNA libraries were prepared using the Chromium Next GEM Single cell 3' Reagent Kit v3.1 (10x Genomics) according to the manufacturer's instructions.