GSM7437018: Med12 in BMDCs experiment, Med12 KO sample3; Mus musculus; RNA-Seq
RNA was recovered using RNeasy plus mini kit (Qiagen #74136). Samples for ATAC-seq were prepared with the kit (Active Motif #53150) according to the instruction manual. Samples for Chip-seq were prepared according to the protocol described by Antman et al  with several modifications. We used 30x10^6 BMDCs for each experiment. Quenching of formaldehyde was done at RT on a gently rocking platform. Samples were sonicated for three cycles (30 s on, 30 s off). For immunoprecipitation, 10 µL of Cebpb antibody was added (ab32358) and 30 µL of Protein G magnetic beads. Then, 2.5% of the sample was taken as input. Chromatin was eluted from the beads by shaking them in a heat block at 800 rpm for 30 min at 65 °C and reverse crosslinking was performed at 65 °C overnight. The libraries for RNA-seq were prepared using KAPA mRNA capture kit (Roche #07962231001) following the manufacturer's instructions. The libraries for Chip-seq were prepared using   KAPA HyperPrep Kit (Roche #7962347001) according to the instruction manual (1 ng of immunoprecipitated sample and 100 ng of input sample were used for library preparation). The libraried for ATAC-seq were prepared using the kit (Active Motif #53150) according to the instruction manual.