GSM8154123: CR, seedlings, rep1; Arabidopsis thaliana; RNA-Seq
Total RNA was extracted  from seedlings using the Direct-zol RNA Miniprep (Zymo Research, Irvine CA, USA) kit with an on-column DNase treatment according to the manufacturer's instructions. The quantity and integrity of RNA were assessed using a NanoDrop ND-1000 spectrophotometer (Thermo Fischer Scientific, Waltham, MA, USA) and agarose gel electrophoresis. RNA-seq libraries were generated using the polyA Strand-Specific Transcriptome Library method of BGI for DNBSEQ platform. Sequencing was performed on DNBSEQ-G400 platform instrument at BGI (Beijing Genomics Institute) in three biological replicates for each sample.