GSM7822295: UHL6; Homo sapiens; RNA-Seq
The qualified cells are washed and resuspended to prepare the appropriate cell concentration of 700-1200 cells/ul for on-board operation of the 10x Genomics Chromium™ system. The following steps, such as generating single cell gel bead emulsions (GEMs), synthesizing complementary DNA, building second generation sequencing libraries were carried out according to the manufacturer's instructions. The resulted libraries were sequenced on the llumina Novaseq 6000 platform with paired end 150 bp sequencing. Each sample was sequenced to depth of ∼120 G of raw reads.