GSM7792337: RIP_4A2_input_rep1; Homo sapiens; RIP-Seq
Cells were directly lysed in lysis buffer on culture dishes. For ribosome profiling (Ribo), RNase I treatment, ribosome pelleting, size selection, preadenylated linker ligation, rRNA depeletion, reverse transcription, cDNA circularization, and PCR amplification. For RNA-Seq (RNA), RNA extraction and TruSeq Stranded mRNA Library Prep Kit (Illumina). For RNA pulldown-Seq (RIP), pulldown by streptavidin beads, elution by biotin, RNA extraction, and TruSeq Stranded mRNA Library Prep Kit (Illumina).