Gene expression changes associated with kinase-inactivated Cdk6 or a complete Cdk6 KO in mouse HSC/MPP1 cells after two rounds of transplantation
Gene expression changes associated with kinase-inactivated Cdk6 or a complete Cdk6 KO in mouse HSC/MPP1 cells after two rounds of transplantation
Mouse HSC/MPP1 cells were isolated from bone marrow by means of flow cytometry sorting using the following immunophenotypic marker combination: lineage-negative, Sca1-positive, cKit-positive, CD150-positive, CD48-negative. This was part of the library construction protocol. Libraries were constructed following the SmartSeq2 protocol as described in Picelli, S., et al. Nature Protocols 9, 171-181, 2014