GSM7067550: ZFP-ETRs against Pcsk9 treated hepatocytes after partial hepatectomy, replicate 1; Mus musculus; Bisulfite-Seq
Genomic DNA extraction by Maxwell® 16 Cell LEV DNA Purification Kit and biisufite conversion by EpiTect Bisulfite Kit, following provider indications. Finally, the promoter region of Pcsk9 was amplified by PCR. Libraries were prepared using NEBNext® Ultra™ DNA Library Prep Kit for Illumina following the manufacturer's recommendations