Full-length transcriptome sequencing of Apis cerana cerana drone
1g total RNA was used for cDNA libraries. Briefly, the template switching activity of reverse transcriptases enrich for full-length cDNAs and add defined PCR adapters directly to both ends of the first-strand cDNA. And following cDNA PCR for 14 circles. The PCR products were then subjected to ONT adaptor ligation. Agencourt XP beads was used for DNA purification according to ONT protocol.