GSM7821519: Male Control Pituitary 15 wk rep1; Mus musculus; RNA-Seq
Pituitaries were removed for dispersion/centrifugation, cells were washed gently, centrifuged, and resuspended in DMEM with no gentamicin. individual pituitary cell suspensions were pooled within treatment groups (2-3 pituitaries/pool) and counted.  Concentration of the cells within each pool was adjusted to optimally target 10,000 cells according to 10X Genomics guidelines.   Samples were then submitted to the UAMS Cancer Institute Genomics Core facility, where cells were resuspended in PBS with 0.04% BSA, filtered, recounted, and assessed for viability.  Then, 10,000 cells from each pool were targeted for scRNA-seq according to the 10X Genomics protocol.