Whole transcriptome sequencing of common bean
Whole transcriptome  and miRNA profiles were determined by the next generation sequencing tools at high temperatures in Perola (which can bloom and fertilize at high temperatures) Karacasehir-90 (non-flowering at high temperature) cultivars. Strand-specific libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) following manufacturers recommendations and index codes were added to attribute sequences to each sample.Briefly, ribosome RNA was depleted from total RNA. Fragmentation was carriedout using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer(5X).