GSM7610658: SG32 PDX dBET6 treated 1; Homo sapiens; RNA-Seq
Snap frozen tissue was homogenised in Trizol using the Precellys cyrolysis system. The aqueous phase was isolated and transferred to a Qiagen Mini kit column following which kit protocol was followed. At least 500ng of RNA was used for library prep. RNA libraries for RNA-seq were prepared usingUltra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) following manufacturer's protocols.