Small RNA sequencing of E. muscae
4 mL of E. muscae liquid culture was pelleted and RNA was extracted with Trizol per manufacturers instructions. The sample was treated with Turbo DNase (CAT #AM2238, Thermo Scientific) and Trizol-extracted again. RNA integrity was confirmed with an RNA 6000 Pico chip (CAT #50671513, Agilent Technologies) on the Agilent 2100 Bioanalyzer. RNA was diluted to 200 ng/l, and 1 ug (5 l) was used as input for the TruSeq small RNA kit (CAT #RS-200-0012, Illumina). The size range was confirmed on the 2100 Bioanalyzer with a HS DNA chip (CAT #50674626, Agilent Technologies). 204.5 million 50 singleton reads were obtained with a HiSeq 4000 (Illumina).