GSM7766628: siNCoR_1; Homo sapiens; ATAC-seq
25.000 FACS sorted cells were collected by centrifugation at 1000g for 5min and used for ATAC Library preparation using Tn5 Transposase from Nextera DNA Sample Preparation Kit (Illumina). Cell pellet was resuspended in 50µl Lysis/Transposition reaction (12.5µl THS-TD-Buffer (Tris-acetate, pH 7.8 132 mM, Potassium acetate (Sigma, 95843-100ML-F) 264 mM, Magnesium acetate (Sigma, 63052-100ML) 40 mM, Dimethylformamide (DMF) (Sigma, Sigma D4551-250ML) 64%, H2O), 2.5µl Tn5, 5µl 0.1% Digitonin and 30µl water) and incubated at 37°C for 30min with occasional snap mixing. Following purification of the DNA fragments was done by MinElute PCR Purification Kit (Qiagen). Amplification of Library together with Indexing Primers was performed as described elsewhere [29].