GSM7152431: DM1; Acinetobacter baumannii AB5075; RNA-Seq
Cultures were harvested by centrifugation (or direct resuspension for desiccated cells) in RNAlater to preserve RNA integrity. RNA was isolated using athe Qiagen RNAeasy Kit with on-column DNAase digestion (Qiagen). RNA quality was assessed using a Bioanalyzer. Samples were additionally treated with RNAse-free DNAse (Invitrogen). Library preparation was performed using Illumina's Stranded Total RNA Prep Ligation with Ribo-Zero Plus kit and 10bp IDT for Illumina indices. Sequencing was done on a NovaSeq 6000 giving 2x51bp reads.