GSM7950686: 20_00196_LI_SING,COPD, scRNAseq; Homo sapiens; RNA-Seq
Immune cells from peripheral blood were isolated by Ficoll density gradient centrifugation protocol. And after two rounds of PBS washing, cell counting was performed. Library was performed according to the manufacter's instructions (Chromium Next GEM single cell 3' v3.1 protocol, 10x genomics). Briefly, GCs were resuspended in the master mix and loaded together with partitioning oil and gel beads into