Capra falconeri
Phenol chloroform (Organic) method was used for DNA extraction.20 mg of Bone marrow was picked and homogenized in 500l of lysis solution and incubated for 20-30 minutes at room temperature. Samples were mixed gently to ensure the proper homogenization. Samples were centrifuged for 3 minutes at 13000 rpm for phase separation. The supernatant was discarded while the pellet containing DNA was further processed, multiple washings with lysis solution were done to prevent contamination. Pellet was again treated with 400 l lysis solution, 13 l of 20% SDS (Sodium Dodecyl sulphate) and 25 l proteinase K. Samples were incubated at 37C overnight.