GSM6806689: IMR90 cells, ER-RAS 3 days 4OHT, Replicate B; Homo sapiens; RNA-Seq
Cells were harvested in TRIzol at the end of treatment and RNA was harvested according to the manufacturer's instructions. Once at the precipitation step, RNA samples were loaded on a RNeasy column (Qiagen Mini Kit #74106). 1000 ng of total RNA was used for the construction of the sequencing libraries. RNA libraries for RNA-seq were prepared using the KAPA mRNAseq Hyperprep kit (KAPA, Cat no. KK8581) following manufacturer's protocols.