PacBio sequencing of Zacco platypus
Muscle tissues were excised from these frozen specimens for the extraction of high molecular weight (HMW) DNA using a conventional CTAB-based method. Genomic DNA libraries were prepared according to the manufacturer's instructions. The single-molecule real-time sequencing (SMRT) bell (SMRT Bell) library was constructed using a PacBio SMRTbell prep kit 3.0 (Pacific Biosciences).