GSM8423043: PBMC_COVID_survivor_40_Day 7; Homo sapiens; RNA-Seq
Total RNA was extracted using the Direct-zol RNA Miniprep Plus kit (Zymo Research) followed in tube DNaseI treatment and RNA cleanup with the Monarch RNA Cleanup Kit (New England BioLabs), per the manufacturer's protocols. RNA-seq libraries were generated using 100ng total RNA and the following kits and conditions: NEBNExt Ultra II Directional (stranded) RNA Library Prep Kit for Illumina, NEBNext rRNA Depletion Kit, and NEBNExt Multiplex Oligos for Illumina Unique Dual Index Primer Pairs (All New England BioLabs).