Processed_seafood_metagenome
Separate COI and 16S PCR were performed. Then, libraries were diluted and pooled to achieve a 100 pM equimolar concentration for the template reaction. Attaching DNA fragments to Ion Sphere Particles (ISPs) for clonal amplification via emulsion PCR, conducted using the Ion Chef System (Thermo Fisher, Monza, Italy). Following preparation, samples were sequenced on the Ion GeneStudio S5 Prime sequencer (Thermo Fisher Scientific, Monza, Italy) utilizing an Ion 530 chip. The sequencing reads were analyzed using Torrent SuiteTM version 5.4 (Thermo Fisher Scientific, Monza, Italy)