illumina RNA sequencing of Zacco platypus
RNA was extracted from muscle tissue using the RNeasy Plus Mini kit, in accordance with the manufacturers instruction. Transcriptome data were obtained with Illumina pairedend sequencing (150bp; Illumina Novaseq platform). The complementary DNA (cDNA) library was prepared using a TruSeq Nano DNA Library preparation kit (Illumina Inc.) according to the manufacturers instructions. Highthroughput sequencing was performed using an Illumina NovaSeq 6000 platform (Illumina Inc.) following provided protocols for 2 x 150 pairedend sequencing.