Rhizosphere_fungi_Vicia sativa
DNA extraction of rhizosphere soil using Dneasy Powersoil Pro Kit (Qiagen). Amplification at Novogene (Cambridge, United Kingdom) of ITS2 region, with the forward primer, 5-GCATCGATGAAGAACGCAGC-3 and reverse primer 5-TCCTCCGCTTATTGATATGC-3.