RNA-Seq of Dabio rerio: whole embryo exposed to sargasso
For RNAseq analysis, we extracted total RNA from three pools of surviving embryos after 96 h of exposure (n = 15 per pool) for control and 200 mg/L treatments, using the Trizol protocol. RNA quantity was determined using a NanoDrop 2000 (Thermo Fisher Scientific), and RNA integrity number (RIN) was determined using the Agilent Bioanalyzer 2100 system (Agilent Technologies) equipped with an Agilent RNA 6000 Nano. We only sequenced RNA samples with RINs greater than 8. Library construction and sequencing were carried out at the Polymorphisms Sequencing and Identification Unit of the National Institute of Genomic Medicine, Mexico City. The cDNA libraries were constructed using the Illumina TruSeq mRNA Sample Preparation Package (Illumina). Products were quantified using an Agilent DNA High Sensitivity Chip on an Agilent Bioanalyzer 2100 System, a Qubit fluorometer, and an Invitrogen from Thermo Fisher Scientific Qubit dsDNA HS Assay kit. Libraries were sequenced on an Illumina NextSeq500 platform with an Illumina 300-cycle NextSeq 500/550 High Output v2 kit in a 2 x 75 bp paired-end output format.