16S rRNA sequencing Phyllospora comosa eggs_2
DNA was extracted using the DNeasy PowerSoil Kit. Bacterial communities were characterized by sequencing of amplicons for the 16S rRNA gene. Fragments of approximately 270 base pairs (bp) were amplified by PCR using the primer pair 305Fw/804Rv (Earth Microbiome Project). PCR products were sequenced using Illumina MiSeq technology (2 x 150 bp) at the Ramaciotti Centre for Genomics (UNSW, Sydney, Australia).