scRNA-seq of mus musculus p53/MCA induced sarcoma tissue
Single-cell suspensions from sorted live CD45+ cells were loaded on a Chromium Controller (10x Genomics) to generate single-cell beads in emulsion, and scRNA-seq libraries were prepared using the Chromium Single Cell 3 Reagent Kits (v3.1 Single Index Kit), Chromium Next GEM Single Cell 3 GEM, Library & Gel Bead Kit v3.1, Chromium Next GEM Chip G Single Cell Kit, and Single Index Kit T Set A (10x Genomics) following the manufacturers protocol. Single-cell barcoded cDNA libraries were qualified and quantified using Agilent Bioanalyzer. cDNA libraries were sequenced on an Illumina NextSeq 500 (Illumina). Read lengths were 26bp for read 1, 8bp for i7 index, and 98bp for read 2. Ten thousand cells from each sample were sequenced with greater than 50,000 reads per cell as recommended by the manufacturer.