Successional and seasonal patterns in the Ulva microbiome
DNA extracted with the QIAamp DNA mini kit (Qiagen) . Full length 16S rRNA gene amplified with primers 27F and 1492R. Amplicons multiplexed with Oxford Nanopore barcodes (EXP-PBC096) and pooled in equimolar ratios. Final library prepared with the ligation-based sequencing kit SQK-LSK109 (Oxford Nanopore). Sequenced on a MinION flow cell (R9.4.1).