GSM6656476: monkey M8020, xenograft biopsy tissue sample after xenotransplantation, day 55; Sus scrofa; RNA-Seq
Total RNA was extracted using the Qiagen RNeasy kit (Qiagen, 74106) after passing the sample through homogenizing columns (Qiagen, 79656) and performing a DNAase digestion (Qiagen, 79256) according to manufacturer's instructions. RNA integrity number and concentration was measured using an automated electronic electrophoresis system (Agilent, 4200 Tapestation) and reagents (Agilent, 5067-5578). For those samples passing quality checks, 500ng of total RNA was carried into the Illumina Stranded mRNA kit library preparation (Illumina, 20040534). Libraries were characterized using the high sensitivity dsDNA assay (Invitrogen, Q33231) for Qubit 3 (Invitrogen, Q33216).