RNAseq of Babraham pig testes
Tissues, processed in triplicate, were disrupted and homogenized in RLT Buffer containing beta-mercaptoethanol (Qiagen, GmbH) using a rotor-stator homogenizer. RNA was extracted and purified using the Qiagen RNeasy Plus Mini Kit which includes a gDNA removal step. Libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina.