RNA-Seq of type 2 diabetic human nasopharyngeal sample
The variable V3-V4 region of the bacterial 16s rRNA gene was amplified by PCR with the primer pair MiSeq341F and MiSeq805R. The 5'-ends were Illumina adapter sequences while the 3'-ends of the primers amplified the V3-V4 region of the 16S rRNA gene. The amplicons were paired-end sequenced (2 x 300bp) USING Illumina MiSeq. Quality control on the pooled library was performed using Qubit and TapeStation.