Embryo_WT
Drosophila stage II embryos and ovary tissues were chosen for small RNA extraction. Small RNAs were purified using miRNeasy mini kit and RNeasy Minelute clean-up kit from qiagen. Illumina Truseq Small RNA Library prep kit was used to prepare the libraries. Prepared libraries were selected for 140 to 160bp products by PAGE and purified. Final purified libraries were checked for quality and pooled in equimolar concentration and loaded on Illumina Nextseq 2000 machine for sequencing. Sequencing was performed in paired-end manner with 50 bp coverage. Approximately 30million reads for each sample were achieved.