GSM7032262: MeA_E2_CRE3; Mus musculus; RNA-Seq
Brains from ovariectomized (OVX) or OVX + Estradiol (E2) treated Kiss1Cre+/Rpl22HA+/+ mice collected and flash frozen. The MeA was bilaterally micropunched from each brain and pooled (n=4 brains per pooled sample). Each pooled MeA sample was run through the Ribotag immunoprecipitation pulldown procedure (Sanz et al., Current Protocols in Neuroscience, 88:e77, 2019) to isolate HA-tagged ribosomes (specific to Kiss1 cells). RNA was extracted from these ribosomes using the Qiagen RNeasy Plus Micro Kit (#74034) per the kit instructions.  Unstranded mRNA library kits from Illumina that used polyA enrichment were used to create the library. The library was created using unstranded mRNA library kits from Illumina that used polyA enrichment.