Extraction from Groundwater collected from Snake Pond on 06/01/2016
The V4 region of bacterial and archaeal 16S rRNA were amplified employing a one-step PCR method using the 515f/806 primer pair and sequenced using MiSeq IlluminaSequencing using a dual-index sequencing strategy and curation pipeline. Detailed descriptions are described by Kozich et al, 2013.