16S DNA-seq of metagenome: activated sludge
16S V3-V4 libraries were prepared using custom fusion primers. The latter include the appropriate P5/P7 Illumina adapter sequence, an 8-nt index sequence, and the gene-specific primer sequence. Libraries were purified with Agencourt AMPure XP beads and validated with an Agilent Technologies 2100 bioanalyzer. Sequencing was performed on Illumina HiSeq 2500 in 2 300 bp mode.