RNA-seq of Drosophila melanogaster: brain
The workflow consists of: first strand cDNA synthesis with oligo(dT) primers containing an Illumina-compatible sequence at the 5 end, RNA template removal, second strand synthesis with random primers containing an Illumina-compatible linker sequence at the 5 end, purification using magnetic beads to remove all reaction components, and PCR amplification in order to add the complete adapter sequences and to generate the final library.