GSM6656056: SMF008_MOLM13_Cas9_NegIAA_NegDOX_Rep2_RNAseq; Homo sapiens; RNA-Seq
Total RNA was extracted by Trizol (Thermo Fisher Scientific, 15596026) from replicate samples. About 200 ng total RNA was treated using Kapa rRNA depletion reagents to remove ribosomal RNA, then converted into cDNA libraries using Kapa RNA HyperPrep Kit with RiboErase (HMR). After the end repair procedure, dA-tailing and adapter ligation, each cDNA library was purified and enriched by 11 cycles of PCR amplification.