pLOvaAlu-14b-1
Alu-Mut plasmid libraries were generated using PCR PCR-based amplification with primers sets described in Supplementary Table 5 using the Ovation low complexity library prep kit (Ovation Library system for Low complexity samples Part no: 9092-16). Briefly, the PCR amplicon was end end-repaired, and forward and reverse diversity adaptors were ligated, filled-in and dual index barcode i5 and i7 primers were used to fill-in and PCR perform PCR for 10 cycles was done to generate the Alu library fragments