metagenome of rumen: hu lamb
The metagenomic DNA extract was fragmented to an average length of about 400 bp using Covaris M220 (Gene Company Limited, China). Paired-end sequencing libraries were constructed using the NEXTFLEX Rapid DNA-Seq kit (Bioo Scientific, Austin, TX, USA) with the adapters containing the sequencing primer hybridization regions ligated to the blunt-end of the fragments. Paired-end sequencing was performed on the Illumina NovaSeq platform (Illumina Inc., San Diego, CA, USA) at Majorbio Bio-Pharm Technology Co., Ltd. (Shanghai, China) using a NovaSeq 6000 S4 Reagent Kit v1.5 (300 cycles)