GSM7884375: LPS_8h_H3K4ac_rep2; Mus musculus; ChIP-Seq
To harvest the samples, 2 μl of DMEM was added to the cells, and formaldehyde was used to cross-link and fix the cells. After 10 minutes of incubation at room temperature, 2.5M glycine was added to quench the formaldehyde. ChIP-seq libraries were prepared using the NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina (NEB, E6240, following the manufacturer's instructions. 10 ng of ChIPed DNA was used as starting material.