PacBio SMRTbell libraries
PacBio SMRTbell libraries (~20kb) for PacBio Sequel was constructed using SMRTbell Template Prep Kit 1.0 (PacBio, Menlo Park, CA, USA) using standard manufacturer protocol. The pooled library was bound to polymerase using the Sequel Binding Kit 2.0 (PacBio) and loaded onto PacBio Sequel using the MagBead Kit V2 (PacBio). Sequencing was performed on 2 PacBio Sequel SMRT cells (Pacific BioSciences, Menlo Park, Can USA).