GSM8197111: RNA Individual 4 skin fibroblast cells, Dexamethasone, Time8; Homo sapiens; RNA-Seq
RNA from cell lysates was extracted using the Qiagen RNeasy mini kit (Qiagen 74106). Cell lysates were extracted in a randomized order to prevent batch effects in downstream analysis. In order to collect total RNA including small RNAs, the standard extraction protocol (Purification of Total RNA from Animal Cells using Spin Technology) was adjusted by making the following changes: (i) adding 1.5 volumes of 100% ethanol, instead of 70%, after the lysis step (step 4 in handbook protocol) and (ii) adding 700 mL of buffer RWT (Qiagen 1067933) instead of the provided RW1 (step 6 in handbook protocol). RNA library preps were made using the Lexogen QuantSeq 3' mRNA-Seq Library Prep Kit