GSM6806326: Polychromatophil, SF3B1 WT rep 4; Homo sapiens; RNA-Seq
The RNA integrity (RNA Integrity Score ≥7.0) was checked on the Agilent Fragment Analyzer (Agilent) and quantity was determined using Qubit (Invitrogen). SureSelect Automated Strand Specific RNA Library Preparation Kit was used according to the manufacturer's instructions with the Bravo Platform. Briefly, 50–100 ng of total RNA sample was used for poly-A mRNA selection using oligo(dT) beads and subjected to thermal mRNA fragmentation. The fragmented mRNA samples were subjected to cDNA synthesis and were further converted into double stranded DNA using the reagents supplied in the kit, and the resulting dsDNA was used for library preparation. The final libraries were bar-coded, purified, pooled together in equal concentrations, and subjected to 100bp paired-end sequencing on Novaseq-6000 sequencer (Illumina) at Gustave Roussy.