GSM7336446: H7 human embryonic stem cells,Replicate 2,S6K1-depleted,Week 14; Homo sapiens; RNA-Seq
For the single cell RNA sequencing, organoids were dissociated with Papain dissociation kit (Worthington Biochemical Corporation, #LK003150). (PN-120263;120212). The single cell droplets were barcoded using Chromium Next GEM Single Cell 3' Kit v3.1 (10x Genomics, cat# 1000121) by subjecting the cells to Chromium Single Cell Controller. Then, cDNA with barcodes was synthesized through reverse-transcription in the droplet and amplified using Chromium Next GEM Single Cell 3ʹ v3.1 kit .