GSM8326473: HEK293T viewpoint MEIS1-AS3 Replica 3; Homo sapiens; OTHER
The primary restriction enzyme digestion was performed with 800 U NlaIII or ApoI (New England Biolabs, R0125 and R3566). Following in situ ligation of digested chromatin, nuclei were lysed and the isolated DNA was digested with CviQI (New England Biolabs, R0639), circularized by ligation and purified. The library was amplified by inverse PCR with viewpoints-specific primers coupled to Illumina adapters for sequencing. Following inverse PCR, the i5 and i7 Illumina adapters were used to generate NGS-compatible libraries as previously described 44. Amplicons were sequenced in a 50 bp paired-end mode on the Illumina NextSeq2000 platform.