full ITS of Vitis vinifera: grape must
Grapes were collected at time of harvest from an organic vineyard then crushed to obtain unfiltered juice (must) which was divided into four replicates. DNA was extracted using the DNeasy UltraClean Microbial Kit. Fungi were characterized by the full ITS region using primer pair ITS1FKYO2 and ITS4KYO1 at the IMR facility at Dalhousie University.