RNA-Seq of ileum microbial : weaned goat
After extracting the total DNA of the sample, the specific primers with Barcode were synthesized according to the 16S full-length primers 27F and 1508R. The PCR amplification was performed and the product was purified, quantified and homogenized to form a sequencing library ( SMRT Bell ). The library was first subjected to library quality inspection, and the qualified library was sequenced by PacBio Sequel.