16S metagenome sequencing of Tenebrionidae sp.
The entire 16S rRNA region was amplified using the 16S Barcoding Kit 1-24 (SQK-16S024, ONT) and Phusion high fidelity PCR master mix with HF buffer (Thermo Fischer Scientific). Purification to clean up all the unused barcodes and dNTPs was done using Ampure XP beads (Agencourt Bioscience) followed by pooling of all the barcoded amplicons together, loaded it in R10 flow cell (FLO-MIN111, ONT) then sequencing was done using MinION device for 4 hours.