GSM7688106: GW19, RNAseq; Mus musculus; RNA-Seq
For RNAseq  for tumor cells, the spleen cells bearing lymphoma were collected, red blood cells were lysed by ACK lysis buffer on ice, washed with cold PBS, passed through a 70-μm cell strainer and used for RNA extraction. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.