16S rRNA: Cement Creek Groundwater Well
DNA was extracted using a ZymoBiomics DNA/RNA MiniPrep Kit and amplified with primers adapted from Parada et al. (2016) as described in Kraus et al. (2018);  targetted the V4 and V% hypervariable regions, producing a ~400 bp fragment; barcoded, purified and pooled amplicons sequenced on an Illumina MiSeq platform using the PE250 V2 chemistry method at the Duke Center for Genomic and Computational Biology.