Long read WGS of Mus musculus: adult male tail
Long read whole genome sequencing libraries targeting N50:20kb reads were prepared using the Ligation Sequencing Kit (Oxford Nanopore Technologies, LSK-110) with 10g of DNA input to achieve N50:10kb median read length. Library preparation followed Oxford Nanopore's recommended procedures, with minor modifications. Samples were aliquoted from the Matrix rack tubes and fragmented using a g-TUBE (Covaris, 520104). After shearing samples were reviewed on the Agilent TapeStation to confirm the desired range, a bead-based cleanup was performed to remove small fragments, followed by another quality control. This was followed by end-prep and nick repair, and then by ligation of sequencing adapters. Final libraries were quantified by Qubit (ThermoFisher, Q32854) prior to being loaded on a 9.4.1 Flow Cell on the Oxford Nanopore Technologies PromethION, one sample per Flow Cell. Nuclease flushes were performed on the PromethION at 24-48 hours.