GSM8197297: poly(A) RNAseq eRF1-dTAG mESCs treated with 1hr 500nM dTAG-13, biological replicate A; Mus musculus; RNA-Seq
After RNase1 digestion of cell lysate, 80S ribosomes were isolated by sucrose density gradient. Then the ribosome protected fragments were isolated via 15% UREA-PAGE gel. Ribosome profiling (small RNA-seq)