RAD-seq of Rehderodendron kwangtungense
Total genomic DNA was extracted from the dried leaves using a modified cetyltrimethylammonium bromide (CTAB) method (Doyle & Doyle, 1987). Qualified DNA (Qubit 3.0) was sent for RADseq library construction following Peterson et al. (2012), specific barcodes were added to each sample and 1024 samples were pooled together to select 350550 bp size as one library template. After PCR amplification, each library was sequenced on an Illumina NovaSeq (Illumina) in PE 150 mode.