GSM8016104: KBP86T1; Mus musculus; RNA-Seq
In order to obtain high-quality and intact RNA from the normal pancreas, the pancreas was perfused with the RNase inhibitor solution RNAlater (Qiagen, 76106) before isolation from the littermate control mice. The isolated pancreas with perfusion was cut into small pieces for total RNA extraction. Total RNAs from normal pancreas and tumor tissues were performed using the RNeasy Mini Kit (Qiagen, Cat#74136) according to the manufacturer's instructions. RNA libraries were prepared for sequencing using standard Illumina protocols at the St. Jude Hartwell Center.