Nucleases activity comparison in HCT116: CasMINI targeting BATF, replicate 1
Transfections were performed with Lipofectamine 2000 (Invitrogen) at a ratio of 2 ul reagent per ug of plasmid and per 24 ul volume of Opti-MEM reduced serum media (Gibco). On a half day prior to transfection, cells (1x10^5) were seeded into 48-well plates. 300 ng of nuclease plasmids and 200 ng gRNA/reRNA plasmids were transfected into HCT116 cells. The transfected cells were collected 2 days post transfection for deep sequencing analyses.