GSM7329891: eyeRibo_nocteRNAi2; Drosophila melanogaster; OTHER
RNA was extracted byTRizol following the product manual. For Ribo-seq, total RNA was digested with Mnase S7 and run using 15% TBE-urea gel, followed by cutting the 28 nt RNA fragments. Library was constructed by standard Illumina protocol. Ribo-seq library was generated by NEBNext® Multiplex Small RNA Library Prep Set for Illumina (E7300S, New England BioLabs).