GSM6805808: RD7-2; Mus musculus; RNA-Seq
Organoid mRNA was isolated using the RNeasy Micro Kit (Qiagen) based on the kit instructions. RNA was reverse transcribed and amplified into cDNA using the Takara SmartSeq kit. Libraries were subsequently constructed with the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB,E7645L).