Rainwater eDNA. Rain containing eDNA collected from the air
Capture and Illumina shotgun sequencing of eDNA from rainwater. Rainwater was collected in sterile DNA-free funnel and bottles as it fell from the sky. Rainwater was then filtered through a 0.22uM filter and eDNA extracted. Shotgun sequencing of all recovered eDNA (metagenomics) with no enrichment or targeting.