Metagenome from human skin
Samples were colleced by swabbing the face with a sterile swab. Whole metagenome sequencing libraries were prepared using the NEBNext Ultra II FS DNA Library Prep Kit (Epicentre, USA). The DNA was purified and size selected to remove excess adaptors and adaptor dimers using Ampure XP beads. Adaptor-ligated DNA was then subjected to PCR amplification with universal primers and an index barcode that was unique for each sample. The PCR products were cleaned and size-selected with beads to get a 250600 bp library.