GSM7434859: HCC1954_KAT6 inhibitor_48hr_R2; Homo sapiens; RNA-Seq
Cells were treated in triplicate with DMSO control or 30 nM PF-9363 for 2 or 5 days and resuspended in RNALater (Ambion #AM-7020) for RNA extraction. At this dose KAT6 biomarker, H3K23Ac is strongly inhibited while having minimal effects on KAT7 biomarker, H3K14Ac (Figure S12). RNA quality was ensured with >8 RIN value using Agilent 2100 Bioanalyzer. cDNA library was generated with TruSeq Stranded mRNA Library Prep kit (Illumina #20020595) and sequenced with Illumina HiSeq X Ten Platform with 150 bp paired-end reads with > 30 million clusters per sample.