GSM7916089: RNA MECP2T203M-1; Homo sapiens; RNA-Seq
Following a standard protocol, total RNA from day-30 organoids was extracted using a TRIzol reagent kit (Invitrogen, Thermo Fisher Scientific). RNA integrity was checked on an Agilent 4200 TapeStation to conduct quality control (Agilent Technologies). Library construction and high-throughput RNA-Seq were performed with Illumina Novaseq6000 sequencer with a sequencing depth of at least 100,000 reads per cell with pair-end 150 bp (PE150) strategy.