FACS sorted RNA-seq of Homo sapiens: female breast epithelial luminal
Breast tissue samples donated to the Brisbane Breast Bank were obtained from healthy donors who had breast reduction surgery. Samples were enriched for epithelial cell populations and suspensions were stained with a lineage marker antibody combination (anti-CD31, anti-CD45 and anti-CD140b) designed to exclude endothelial, hematopoietic, and leukocyte cells. The remaining lineage-negative cell superpopulation was sorted into basal (EpCAMlow/CD49fhi), luminal progenitor (EpCAMhi/CD49fhi), and luminal (EpCAMhi/CD49flow) cell types. Total RNA was extracted, ribodepleted  [Ribo Zero Gold kit] and pooled into 3 separate biological replicates (i.e. each pooled sample combined 4 donors).