GSM6415314: senescent HDFs, MP470, bio rep 2; Homo sapiens; RNA-Seq
Total RNA was extracted from HDFs using the QIAzol Lysis Reagent on ice, following the manufacturer's protocol. RNA purity and concentration were determined using NanoDrop One spectrophotometer. Integrity of RNA was also confirmed by gel electrophoresis and BioAnalyzer before RNA-Seq. Isolated RNA was fragmented using TruSeq Stranded mRNA LT Sample Prep Kit according to the manufacturer's protocol, and libraries for sequencing were then generated based on NovaSeq 6000 S4 Reagent Kit and the protocols in Microgen Inc. (Seoul, South Korea). The libraries were sequenced using NovaSeq 6000 system.