GSM8154184: A549 cells, STAT1g1, mock, rep1; Homo sapiens; RNA-Seq
Total RNA was isolated from cells following the TRIzol isolation method (Invitrogen). All samples were quantified and assayed to confirm minimum RNA integrity number of at least 9.4 using an Agilent Bioanalyzer (RNA 6000 Pico Kit, catalog no. 5067-1513). 500 ng of total RNA per sample underwent mRNA capture and was then fragmented at 94°C for 6 min. Sequencing libraries were prepared according to the manufacturer's protocol using eleven cycles of final amplification (KAPA mRNA HyperPrep Kit, catalog no. KK8580 and KAPA UDI Adapter Kit, catalog no. KK8727). Libraries underwent QC prior to sequencing using an Agilent Bioanalyzer (High Sensitivity DNA Kit, catalog no. 5067-4626).