GSM7437229: THP-1 cells, nano extracellular vesicles, 1; Homo sapiens; miRNA-Seq
Exosomal RNA was extracted by the MagZol (Magen) according to the manufacturer's protocol Briefly, rRNAs were removed fromExosomal RNA using QIAseqFastSelect RNA Removal Kit（QIAGEN，Germany）and fragmented to approximately 200bp. Subsequently, the RNAfragments were subjected to first strand and second strand cDNA synthesis following by adaptor ligation and enrichment with a low-cycle according to instructions of NEBNext® Ultra™ RNA LibraryPrep Kit for Illumina (NEB, USA)