GSM7822299: +/+_cre Rep4; Mus musculus; RNA-Seq
RNA was extracted using the EZ-10 DNAaway RNA kit (Bio Basic), according to the manufacturer's protocol, and quantified with NanoDrop (ThermoFisher Scientific). cDNA was prepared using the Moloney murine leukemia virus (MMLV) reverse-transcription kit and quantitative PCRs run on a StepOnePlus instrument with PowerSYBR master mix (all from ThermoFisher Scientific). Primers were purchased from Integrated DNA Technologies. RNA yields and quality were assessed using Bioanalyzer (Agilent). rRNA depletion and library preparation were performed using the KAPA RNA HyperPrep Kit with RiboErase (Roche).