GSM8328284: UHR ERCC spike-in BS-L017_27; synthetic construct; RNA-Seq
1% dilution of ERCC RNA Spike-In Mix (4456740, ThermoFisher) The linear library pool prepared above was amplified with the KAPA HiFi HotStart Library Amplification kit with Primer Mix (#KK2621) to ensure blunt-end products prior to circularization (90s at 98°C; 5 cycles of 30s at 98°C, 30s at 60°C, 30s at 72°C; and 60s at 72°C). Circularization was performed using the Element Biosciences Adept Library Compatibility Kit v1.1 (Cat#: 830-00007) according to manufacturer instructions. The circularized library was quantified using qPCR with the provided standard before being sequenced on the AVITI system with 2 x 150 length paired end reads.