GSM6654231: shcontrol_2; Homo sapiens; RNA-Seq
RNA was harvested using Rneasy mini plus kit (Qiagen). 1.3 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® following manufacturer's protocols. Total RNA qualification mRNA enrichment Double-strand cDNA synthesis End repair, ploy-A & adaptor addition Fragment selection and PCR Library qualty assessement