DNA-Seq non-dysgenic male primordial germ cell 10
Single primordial germ cells were isolated from dysgenic and non-dysgenic hybrid Drosophila melanogaster embryos (11-15 hours old) by FACS sorting. DNA extraction and whole-genome amplification from sorted cells was performed using the REPLI-g Advanced Single Cell kit (Qiagen). DNA was quantified using the Qubit Broad Range dsDNA kit (Invitrogen). Library preparation was performed on 500 ng of whole genome amplified DNA using the Illumina DNA Prep kit according to the manufacturers instructions. Libraries were multiplexed and sequenced as paired-end, 150-nt reads on an Illumina NovaSeq 600 platform.