metagenome of enrichment culture: petroleum culture from hot spring
Following the centrifugation of 50 mL clay suspension at 5000g for 10 minutes, Genomic DNA was extracted from the resulting pellet utilizing the Qiagen soil extraction kit (Qiagen, 47014, Valencia, CA). High-quality DNA was forwarded to Novogene Bioinformatics Technology Co., Ltd (Beijing, China), where sequencing libraries were crafted with a 350bp insert size. Subsequently, the qualified libraries underwent pooling and were sequenced on Illumina platforms using the PE150 strategy, tailored to meet the specific requirements of effective library concentration and desired data volume.