GSM8328309: human neurons, differentiated, F12468, 2-week, rep1 (ATAC-seq); Homo sapiens; ATAC-seq
Generally, live cells were washed once with ice-cold 1xPBS containing protease inhibitor then resuspended in ice-cold nuclei extraction buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 3 mM MgCl2, 0.1% Igepal CA630, and 1× protease inhibitor) for 5 min. Next, counted 50,000 cells per reaction and exchanged into 50 μL 1× buffer TD, and added 2.5 μL TDE1 enzyme for 30 min at 37 °C with rotation for transposition. The fully transposed DNA was purified with Qiagen MinElute spin columns (Qiagen#28006), PCR amplified, and AMPure XP beads size-selected for fragments between 300 and 1,000 bp. Libraries were subjected to paired-end 150 bp sequencing on the Novaseq 6000. ATAC-seq was performed by using the Nextera DNA Library Prep Kit (Illumina FC-121-1030).