genome sequence of NGB-FR 79
Bacterial DNA was extracted from freshly sub-cultured colonies using the QIAamp DNA Mini Kit (Qiagen, cat # 51304) according to the manufacturers instructions, and the DNA concentration was measured using a Denovix Fluorometer (Denovix, USA). The genomic DNA was stored at -20C. Total of bacterial DNA (1 ng) was used in the library preparation. The library was prepared with Nextera XT DNA Library Preparation Kit (FC-131-1096, Illumina, USA), according to the manufacturers instructions. In brief, transposons were used to fragment the DNA, subsequently adapter sequences were added onto the DNA template, the product was then size-selected for optimum insert length, enriched and quantified. Sequencing was carried out with the MiSeq reagent kit 600 v3 (Illumina, USA) on the Illumina MiSeq, generating, on average 301 basepair paired-end reads.