GSM7991383: PFAS-2_replicate-1 [run66_s016]; Danio rerio; RNA-Seq
Total RNA was isolated from pooled animals in Eppendorf safe-lock tubes by euthanizing on ice, immediately removing excess water, homogenizing in 200 µL RNAzol®RT (Molecular Research Center) with 100 µL 0.5 mm zirconium oxide beads (Next Advance) using a Bullet Blender® (Next Advance) at speed 8 for 3 minutes. After homogenization, 300 µL RNAzol® was added, and samples were stored at -80 °C. Isolation was performed using Direct-zol RNA MiniPrep kit (Zymo, Cat no. R2052) and optional DNase I digestion step. RNA libraries for RNA-seq were prepared usingLexogen QuantSeq 3' mRNA-seq FWD kit following manufacturer's protocols.