fungal rhizosphere of Acacia saligna at depth 10-30 cm
Soil DNA was extracted from 0.5 g soil using an Exgene soil DNA mini kit from GeneAll (Seoul, Korea) and stored at 20 C until PCR amplification. DNA was amplified using SimpliAmpTM thermal cycler (Thermo Fisher Scientific, Walham, MA, USA), by mixing 12.5 L HS Taq Mix Red (PCR Biosystems, London, UK), 9.5 L ultrapure water, 1.0 L extracted DNA, 1 L CS1-ITS2 (ACACTGACGACATGGTTCTACAGCATCGATGAAGAACGCAGC), and 1 L CS2-ITS4 (TACGGTAGCAGAGACTTGGTCTTCCTCCGCTTATTGATATGC). The thermal cycling program was set to: 95 C for 2 min, followed by 40 cycles at 95 C for 15 s, 50 C for 30 s, 72 C for 30 s, and after these cycles, 72 C for 3 min. The amplified DNA was stored at 20 C until sequencing.