GSM6605049: PD1lo P14 cells from TdLN-rep1 (D23); Mus musculus; ATAC-seq
Briefly, 50,000 target cells were washed with PBS and then treated with lysis buffer, followed by labeling with the Nextera enzyme according to standard Illumina protocols (15027865; Illumina). The labeled samples were immediately amplified by 9–10 cycles of polymerase chain reaction (PCR) with barcoded primers and sequenced with a NovaSeq 6000 instrument in a 150 bp/150 bp paired-end run using standard Illumina protocols.