GSM7435299: E18.5_mut2_cell17; Mus musculus; RNA-Seq
Embryonic day 18.5 (E18.5) pups were extracted after sacrificing SstCre/+::R26R CAG-boosted eGFP (RCE)/+::Sox6fl/± females with plugged pregnancies. The neocortices of the pups were dissected in ice-cold HBSS. Genotyping of the pups were done in parallel by using QuickExtract DNA Solution (Lucigen). The tissue was dissociated into a single cell suspension, as previously described (Muñoz-Manchado et al. 2018; DOI: 10.1016/j.celrep.2018.07.053). Briefly, the tissue was dissociated using the Papain dissociation system (Worthington) following the manufacturer's instructions. Cell suspension obtained was filtered with 20 μm filter and kept in cold oxygenated Hank's balanced salt solution (HBSS) with 0.2% Bovine Serum Albumin (BSA). EGFP positive but DRAQ7 negative (dead cell marker, Invitrogen) cells were FACS sorted into 384-well plates containing lysis buffer and processed in large accordance with the Smartseq2 protocol (Picelli et al. 2014; DOI: 10.1038/nprot.2014.006). The libraries were generated according to the Smartseq2 protocol (Picelli et al., 2014; DOI: 10.1038/nprot.2014.006). The single-cell transcriptome data was generated at the Eukaryotic Single-cell Genomics facility at Science for Life Laboratory in Stockholm, Sweden.