GSM7104823: NK92_SAHA_RNA_rep2; Homo sapiens; RNA-Seq
RNA was extracted from cells based on chloroform-phenol/guanidine. Total RNA prepared with rRNA depletion and PolyA RNA was isolated by oligo dT beads. cDNA is amplified through 9 cycles of PCR amplification using the NEBNext Ultra RNA Library Prep Kit for Illumina. Libraries were paired-end sequenced on Illumina HiSeq 2500 platform.