Seq of bacteria: crude oil
Total genomic DNA was extracted from the samples using the FastDNA SPIN Kit for Soil (Bio101, Qbiogene, Heidelberg, Germany) according to the manufacturers instructions. The DNA samples were stored at -20 C or analyzed immediately. Strains 2A and 2B were identified by 16S rRNA gene sequencing (ABI 3730 DNA Analyzer, Life Technologies, USA).