GSM7336787: Rubella virus infected cortical organoids replicate 1; Homo sapiens; RNA-Seq
Two organoids per experimental condition were washed with Ca2+/Mg2+-free DPBS and cut into 1 mm2 pieces   and   enzymatically   digested   with   papain   digestion   kit   (Worthington,   LK003163)  with   the addition of DNase for 1 hr at 37C. Two organoid lines with and without microglia that were treated with RV or uninfected. Cell supernatant were combined and captured across seven lanes of 10x Genomics using Chromium single cell 3' reagent kit (v2 Chemistry) following the manufacturer's protocol.  Multi-seq library construction was performed with standard protocol.