leaves_0h_rep2
Samples were collected for RNA sequencing, flash-frozen in liquid nitrogen. Total RNA was extracted from frozen plant tissues using Trizol reagent. cDNA libraries were constructed using the Illumina TruSeq RNA preparation kit (Illumina Inc., San Diego, CA, USA). After second-strand cDNA synthesis, the cDNAs were fragmented, ligated with adapters, and amplified by PCR.