GSM7854584: oocyte, goat_0h_rep3; Capra hircus; RNA-Seq
Samples were collected in tubes with lysis component and ribonuclease inhibitor.Then we carried out the amplification by the Smart-Seq2 method.An Oligo-dT primer was introduced to the reverse transcription reaction for first-strand cDNA synthesis,followed by PCR amplification to enrich the cDNA and magbeads purification step to clean up the production. RNA libraries were prepared for sequencing using standard lllumina protocols.