Illumina NovaSeq 6000 paired end sequencing; RNA-seq of retinal pigment epithelium (RPE) derived from human induced pluripotent stem cells (iPSCs): healthy control and USH1B patient iPSCs
RNA-seq of retinal pigment epithelium (RPE) derived from human induced pluripotent stem cells (iPSCs): healthy control and USH1B patient iPSCs
iPSC-derived retinal pigment epithelium samples were collected in 1.5mL eppendorf tube and snap frozen on dry ice before stored at -80 degree until use Total RNA was extracted using miRNeasy micro kit (Qiagen, 217084) Libraries were prepared using KAPA mRNA HyperPrep Kit (Roche, KK8580) according to manufacturerâ€™s instructions, Quality of libraries was tested using High Sensitivity Agilent DNA 1000 assay (Agilent, 5067-4626)) on Agilent TapeStation 4200