Whole genome lake sample
The DNA was quantified using Qubit nd fragmented to approximately 550 bp using a Covaris M220 with microTUBE AFA Fiber screw tubes and the settings: Duty Factor 10%, Peak/Displayed Power 75W, Cycles/burst 200, Duration 40s and Temperature 20C. The fragmented DNA was used for metagenome preparation using the NEB Next Ultra II DNA library preparation kit. The DNA library was paired-end sequenced 2 x 150 bp on a HiSeq system