Hic of gallua gallus:pectoral muscles in the eighth year of life
Biotinylated DNA fragments were pulled down with streptavidin beads and prepared for Illumina library construction, which was performed with an NEBNext DNA library Prep Kit, followed by a final amplification with Herculase II Fusion DNA Polymerase and purification with Agencourt AMPure XP beads. The resulting in situ Hi-C library was quantified and sequenced on an Illumina HiseqTen platform with a 150 bp paired-end length.