DNA-seq of Pithecellobium dulce: complete chloroplast genome
Genomic DNA was extracted from desiccated leaf tissue employing the modified CTAB protocol. Subsequently, the Genomic Purification Kit was used to purify DNA samples. Assessment of DNA purity, concentration, and integrity was conducted using the NanoDrop OneC UV-Vis Spectrophotometer, Qubit 4 Fluorometer, and electrophoresis, respectively. Libraries were then prepared according to protocol of NEBNext Ultra II DNA Library Prep kit. Approximately 5Gb data (total genomic data) was generated using the Illumina platform at KTest Science Co Ltd. (HCMC, Vietnam).