Cellular traits regulate fluorescence-based light-response phenotypes of coral photosymbions living in-hospite
For each sample, PCR was first 248 performed targeting the internal transcribed spacer 2 (ITS2) region using the previously 249 established primer pairs (ITSintfor2: 5GAATTGCAGA ACTCCGTG-3, ITS2-reverse: 250 5GGGATCCATA TGCTTAAGTT CAGCGGGT-3) (LaJeunesse et al., 2002; Sheffield et al., 251 1989). AGAGACAGGAATTGCAGAACTCCGTG; Reverse-GTCTCGTGGGCTCGGAGATGTGT 254 ATAAGAGACAGGGGATCCATATGCTTAAGTTCAGCGGGT). Adapter sequences are 255 underlined in the above primer sets.