GSM7993439: P90_CUTNTAG_heaEC_P30Tam_WT_r2; Mus musculus; OTHER
Cardiac EC were isolated based on MACS-magnetic MicroBeads technology from Miltenyi Biotec and FACS sorted for CD146 for a 80-90% purity. Isolated cardiac EC were used for cut and Tag standard protocols. Briefly, cells were incubated with SOX9 primary antibody (Millipore, AB5535), followed by a secondary antibody conjugated to the protein A-Tn5 fusion protein. The transposed DNA was purified and amplified by PCR, and the resulting library was sequenced at 100bp paired end.