GSM8197807: Bacteria-injected larvae, 5 day + 6h, replicate 1; Hermetia illucens; RNA-Seq
After 2 h, 6 h and 13 h post-injection, five samples (of two pooled larvae each) were collected for each time point and flash-frozen in liquid nitrogen. Total RNA was extracted with TriZol reagent and subjected to DNase treatment, protein purification and stored at -80 degree C. The quality of total RNA was evaluated using Agilent Bioanalyzer and DeNovix Bioanalyzer. Library preparation was performed at BaseClear B.V. (Leiden, Netherlands). Sequencing libraries were sequenced paired-ended (150 bp read-length) on an Illumina NovaSeq 6000 platform.