single-cell ATACseq of Human lung tissue
Droplet-based scRNA-Seq 10x Genomics V2 (donor samples from Hu28, Hu30, Hu32, Hu37, Hu39, and Hu49 ) or V3 (donor samples from Hu52 and Hu62) 3 gene expression technology was used according to manufacturers recommendations. In short, single cells were washed with 0.4% BSA in PBS, and loaded onto a Chromium single-cell 3 Chip. Single cells were partitioned into droplets with gel beads in the Chromium Controller. After emulsions were formed, barcoded reverse transcription of RNA took place. This was followed by cDNA amplification, fragmentation and adapter and sample index attachment. Libraries were pooled together and sequenced on an Illumina NextSeq 500 with paired end reads.