RNAseq of human tumors from NSG mice
Tumor cell lysates were prepared in TRIzol after powdering the tissue in LN2.  RNA was isolated using the Direct-zol RNA MiniPrep Kit and RNA quality was assessed using an Agilent TapeStation. Sequencing libraries were prepared using the TruSeq Stranded mRNA kit (Illumina, San Diego, CA) (polyA enrichment).