PacBio SMRT seq of Lassa virus NPmt S
Vero cells was infected by rLASV NPD389A mutantat a MOI of 1. At 48 hpi, total RNA was purified from infected cells. The cDNA of S genomic RNA was generated by reverse transcription with a primer targeting 3' end of S gRNA. PCR amplification of the close-to-full-length SgRNA was performed. PCR amplicon (approximately 3.4 kb) was gel purified using Monarch DNA gel extraction kit (NEB).