16SrRNA Water Microbiome 7.8
Before the end of each dive, an ambient seawater sample (1L) was collected from the mid-lower depth (2-3m) of the water column. Water was filtered using 0.45m filter paper (0.5 L of water per filter paper) and each placed into a 1.5 mL centrifuge tube. DNA extractions were performed on octopus skin swabs, seawater, and sediment (0.25g) samples using the Qiagen DNeasy PowerSoil Kit. The DNA samples were prepared for targeted sequencing (16S rRNA gene) with the Quick-16S NGS Library Prep Kit (Zymo Research, Irvine, CA). The primer set used was to target and amplify the V3-V4 region.