Illumina HiSeq 4000 paired end sequencing; Reducing cell intrinsic immune responses to mRNA vaccine constructs improves adaptive immune response in mice
Reducing cell intrinsic immune responses to mRNA vaccine constructs improves adaptive immune response in mice
Mice were culled using 100 Î¼l intraperitoneal pentobarbitone (20 mg dose, Pentoject, Animalcare Ltd. UK) and lymph node tissues were collected as previously described RNA was extracted by the Trizol method after tissue disruption using a TissueLyzer (Qiagen). RNA concentrations were determined using a Nanodrop (ThermoFisher) before sending for sequencing to Imperial BRC Genomics Facility sequencing facility. RNA quality check and library preparation was performed by Imperial College London using the Illumina PE150 at a target depth of 50 million 100-150 base pair (bp)  end reads per sample.