GSM8197239: IAH8R-BS-RNA-seq-rep2; Homo sapiens; OTHER
Zymo Direct-Zol RNA Microprep Kit and Promega mRNA isolation Kit for RNA-seq For RNA-seq, about 300 ng mRNA of each sample was used for library preparation according to the manufacture's protocol of KAPA mRNA HyperPrep Kit (Illumina Platforms). 10cycles of PCR amplification were performed. KAPA Pure Beads (Kapa Biosystems) were used for library purification. The quality of each library was verified using a Qubit fluorometer (Thermo Fisher Scientific) and further evaluated by the 2100 Bioanalyzer (Agilent Technologies).