GSM7065759: 053-5173-1; Homo sapiens; RNA-Seq
18mLs of whole blood was drawn into 3.2% sodium citrate tubes (BD Vacutainer). Blood was centrifuged to obtain platelet rich plasma, and then depleted of leukocytes and RBCs using StemCell EasySep CD45 and RBC magnetic depletion kits. Purified platelets were pelleted and lysed with 500uL of Qiazol lysis buffer and stored at -80 until ready for RNA extraction. RNA extraction was done by utilizing Zymo Microprep kits (R2062), and stored again at -80 until ready for sequencing. Sequencing libraries were barcoded and prepared using the Clontech SMART-Seq HT with Nxt HT kit (Takara Bio USA), and libraries sequenced single end on an Illumina NovaSeq 6000