GSM6806256: Fat1, EtOH replicate 3; Mus musculus; RNA-Seq
Total RNA was purified from liver tissue and BMDMs with Trizol reagent (Thermo Fisher Scientific) as described by the manufacturer, and any contaminating genomic DNA was removed by digestion with DNase I (Thermo Fisher Scientific). cDNA was synthesized with qScript cDNA Supermix (Quanta Biosciences, Beverly, MA) from 1 μg of RNA. Liver RNA was extracted and purified via Trizol Reagent (Thermo Fisher Scientific, Waltham, MA) as described by the manufacturer.  Any carryover genomic DNA was removed by digestion with TURBOÔ DNase I (Thermo Fisher Scientific) followed by further purification with the RNeasy MinElute Cleanup Kit (Qiagen, Germantown, MD). RNA integrity numbers were a minimum of 7.5 as determined on the Agilent Bioanalyzer instrument (Agilent Technologies, Santa Clara, CA). RNA samples were then provided to GENEWIZ (South Plainfield, NJ) for RNAseq. RNA was subjected to poly-A selection followed by 2X150 paired end sequencing on the Illumina HiSeq platform