Oxford Nanopore sequencing of single adult male Tanytarsus gracilentus
We extracted high-molecular-weight DNA from a single adult male using a previously published protocol (doi: 10.17504/protocols.io.mrxc57n). Library preparation and sequencing were performed by the Roy J. Carver Biotechnology Center, University of Illinois Urbana-Champaign. They used 50ng size-selected DNA to create a PCR library with the EXP-PBC001 and LSK-109 kits (Oxford Nanopore Technologies, Oxford, UK) and sequenced the library on one R9.4.1 FLO-MIN106 RevD SpotON flowcell for 72 hours on a MinION II sequencer.