RNAseq of Alopecurus myosuroides seeds
Messenger RNA was enriched by polyA isolation using an NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs (NEB), Massachusetts, USA). Libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit (NEB) with Sample Purification Beads and in-house indexes. A total of 50 libraries (5 replicates per treatment) were sequenced at 8 libraries per lane using an Illumina HiSeq X platform (Illumina Inc., California, USA) generating ~40 million 150 bp paired end reads (~80 million total) per sample