Illumina DNA-Seq w;TM2/TM6 female flies
Genomic DNA was obtained from 10 non-virgin female adult flies. DNA was extracted using the Quick-DNA Microprep kit (Zymo) and quantified by Qubit (Invitrogen). Library preparation was performed on 500 ng of genomic DNA using the Illumina DNA prep kit according to the manufacturers protocol. Libraries were multiplexed and sequenced in paired-end, 150-nt long reads on an Illumina NovaSeq 6000 platform.