GSM8327251: HMV-II cells shEZH2 rep1; Homo sapiens; RNA-Seq
Total RNA was extracted from the cells using TRIzol reagent according to the manufacturer's instructions. The mRNA was purified from total RNA by using poly-T oligo-attached magnetic beads. The library fragments were purified with the AMPure XP system (Beckman Coulter, Beverly, USA). Then PCR amplification, the PCR product was purified by AMPure XP beads, and the library was finally obtained. After the construction of the library, the insert size of the library was detected by the Agilent 2100 bioanalyzer. The library was sequenced by the Illumina NovaSeq 6000. FeatureCounts (v1.5.0-p3) was used to count the reads numbers mapped to each gene. Then FPKM of each gene was calculated based on the gene length and reads count mapped to this gene.