GSM7687988: BY4741, dIoc3, dataset3, MNase-seq; Saccharomyces cerevisiae; MNase-Seq
Cells were harvested and converted to spheroplasts using 0.25 mg ml–1 zymolase100T (MP Biomedicals cat#320932). Spheroplasts were digested with micrococcal nuclease (NEB M0247S) at concentrations of 600/1200/2400 gel unit/ml. Samples were treated with proteinase K (25 μg ml–1, Tiangen RT403) 30 mins at 65 °C to remove protein. Phenol:chloroform was used to extract the DNA. We incubated the sample with RNase A (100 μg ml–1, Tiangen RT405) to remove RNA. DNA libraries were prepared using NEBNext Ultra II DNA Library Prep Kit (NEB #E7645S)