Diet-omics in the Study of Urban and Rural Crohn disease Evolution (SOURCE) cohort
DNA was purified using the PowerMag Soil DNA isolation kit (MoBio) optimized for the Tecan automated platform. DNA was diluted to 1.5 ng, and Illumina libraries were prepared using Nextera DNA library preparation kit, Ref# 15028211; by Tecan Freedom Evo 200 robot device. Nextera DNA Unique Dual Indexes Sets AD from IDT were used for library preparation. Library concentration was measured using the iQuantTM dsDNA HS Assay Kit, ABP biosciences (Cat# AP-N011), and library size was quantified by automated electrophoresis nucleic acid QC -Tape-Station system. Libraries were sequenced by a NextSeq 500 device with IlluminaNS 500/550 High Output V2 75 cycle kit, Cat# FC-404-2005.