GSM7792343: Ribo_4A2KO_PP242_10uM; Homo sapiens; OTHER
Cells were directly lysed in lysis buffer on culture dishes. For ribosome profiling (Ribo), RNase I treatment, ribosome pelleting, size selection, preadenylated linker ligation, rRNA depeletion, reverse transcription, cDNA circularization, and PCR amplification. For RNA-Seq (RNA), RNA extraction and TruSeq Stranded mRNA Library Prep Kit (Illumina). For RNA pulldown-Seq (RIP), pulldown by streptavidin beads, elution by biotin, RNA extraction, and TruSeq Stranded mRNA Library Prep Kit (Illumina).