GSM8425844: B3.R, mRNA-seq; Homo sapiens; RNA-Seq
RNA was isolated using Trizol and RNA Clean & Concentrator Kits (Zymo Research). 1.5 ug of total RNA was used for the construction of sequencing libraries Standard specific mRNA-sequencing (mRNA-seq) libraries were generated using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Ipswich, MA), and single-end libraries were sequenced on an Illumina SE100 Platform with an output of ~680M reads per sample.