Replicate2_RNAseq_HAP1_loxPsym301_scrambled_clone12
RNA containing polyA tails is pulled down using oligo d(T) beads. During conversion to cDNA, uracil is incorporated into one of the strands giving the libraries directionality. During the PCR step, unique dual indexes are applied to allow the samples to be multiplexed ready for sequencing (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina).