GSM7915816: Muscidifurax zaraptor, 24-hour adult male, on fleshfly hosts, replicate 2; Muscidifurax zaraptor; RNA-Seq
RNA extractions were performed with AllPrep DNA/RNA Mini Kit (Qiagen, MD, USA) following the manufacturer's protocol. For RNA-seq library preparation, 1 μg of total RNA was used as input for all samples. To remove the abundant rRNA (ribosomal RNA) in the sample, an rRNA removal protocol was performed using the NEBNext rRNA Depletion Kit (New England Biolabs, MA, USA). The remaining mRNA was used for RNA-seq library construction using NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs, MA, USA) with the manufacturer-provided protocol. After quality control, the RNA-seq library was sequenced on an Illumina NovaSeq6000 platform.