GSM7754659: sgFXN_Rep1; Homo sapiens; OTHER
Genomic DNA was isolated using the XL Maxi NucleoSpin Blood kit (Macherey-Nagel) according to the manufacturer's protocol sgRNA sequences were amplified from gDNA by PCR using Ex Taq DNA polymerase (TaKaRa). For each reaction, a maximum of 10 µg gDNA was used with primers containing barcodes and Illumina sequencing reactions, and amplified with 28 PCR cycles.