GSM6652420: SG#45_26 x day 7 spinoids; Homo sapiens; RNA-Seq
For single cell preparation 26 spinoids at day 7 derived from WTS2 were dissociated frozen and stored at -80. Frozen cells were thawed in 37°C water bath, resuspended in culture medium (N2B27) and spun at 500g for 7 minutes at R/T Cells were resuspended in N2B27 medium with 10µM Y-27632 at density of 700-1200cells/µl and processed for single cell RNA sequencing according to 10X Chromium Next GEM Single cell 3' v3.1 protocol Libraries were prepared following 10X Chromium Next GEM Single cell 3' v.3.1 manufacturer protocol 10X Chromium Next GEM Single cell 3' v.3.1