GSM7437625: male, WT, 13-weeks-old, quadriceps rep3; Mus musculus; RNA-Seq
Frozen quadriceps were homogenized in mL trizol reagent for RNA extraction. RNA was separated with chloroform, precipitated with isopropanol, and washed with 75% ethanol. Total RNA was suspended in RNase Free water.  Total RNA was submitted to Lexogen Gmbh (Vienna, Austria) for 3' mRNA sequencing. Sequencing-ready libraries were produced using a QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (015UG009V0252) using 50 ng RNA per sample. Sequencing was performed on an Illumina NextSeq 500 sequencer with a v2 SR75 High Output kit at Lexogen GmbH.