Human gut metagenome
his metagenomic dataset was generated using second-generation sequencing. Briefly, the metagenomic DNA was extracted utilizing the Magnetic Bead Method for Soil and Fecal Genomic DNA Extraction Kit (DP712; TIANGEN Biotech (Beijing) Co., Ltd., Beijing, China). The purity and integrity of the extracted DNA were evaluated using 0.254% agarose gel electrophoresis, while the quantity of extracted DNA was estimated using the Qubit dsDNA Quantification Assay Kit (Thermo Fisher Scientific Inc., Waltham, MA, USA) in accordance with the manufacturers instructions. DNA libraries were then prepared using NEBnext Ultra II DNA Library Prep Kit (New England BioLabs, Ipswich, MA, USA).