A new Bacillus thuringensis with toxicity against many lepidoptera pests
DNA fragments are coupled with two oligonucleotides adapters carrying specific sequences that enable the subsequent amplification and sequencing steps in the Illumina sequencing systems. During library preparation, short individual barcode sequences (indexes) are also added to the standard adapters in order to allow the simultaneous sequencing of DNA fragments from different samples (multiplexing).The sequencing library is generated by random mechanical fragmentation of the DNA sample (usually with an ultrasonicator), followed by 5and 3 adapters ligation. Genomic fragments are subsequently amplified using primers targeted to adaptor sequences.