GSM7065802: MARV_20h_2; Homo sapiens; RNA-Seq
Total RNA was extracted 20 and 40 hours post infection by Qiagen RNeasy Maxi Kit (Qiagen, Germany), including on-column removal of DNA by digestion with rDNase for 15 min at room temperature, and 1000 ng (each showing a RIN score from >9) were used for library preparation. After depletion of ribosomal RNA (NEBNext rRNA Depletion Kit, #E6310, New England Biolabs, Germany) directional libraries were prepared with a NEBNext® UltraTM Directional RNA Library Prep Kit for Illumina® sequencing (#E7420S, New England Biolabs, Germany).