GSM7855209: Gene expression, scRNAseq; Homo sapiens; RNA-Seq
For each sample, cells were washed with filtered PBS and dissociated using Versene solution containing 0.25% Trypsin and 10 µM ROCK inhibitor Y-27632 followed by RPMI-1640 medium plus FBS at a 1:1 ratio. Cells were then analysed for live/dead cell staining using Zombie NIR™ Fixable Viability Kit (BioLegend). 5 x 105 cells from each live/dead stained sample were collected and pooled. Finally, total of 5 x 105 of single viable cells were sorted from the pooled population using BD FACSymphony™ S6 Cell Sorter. Library was performed according to the manufacturer's instructions (Chromium Single Cell 3' Library, Gel Bead and Multiplex Kit). The sorted single cell suspensions were loaded onto 10x Genomics Single Cell 3' Chips to form single cell gel beads in emulsion (GEMs).