GSM7610698: Radiation Control; Mus musculus; RNA-Seq
Whole BM was flushed from femus and tibias into HBSS. After RBC lysis, cells suspensions were stained and 7-AAD- CD11b+ Ly6C+ Ly6G- cells were sorted on a BD FACSAria Cell Sorter. Cells from each experimental group (radiation and SAA, n = 3 mice per group) were pooled together. Library construction was performed according to the manufacter's instructions (single cell 5' v2 protocol, 10x Genomics). Briefly, BM cells were resuspended in the master mix and loaded into the chromium chip with partioninig oil to generate the gel bead-in-emulsion (GEM). Reverse transcription was then performed and cDNA cleaned up with Silane DynaBeads and amplified by PCR. Library construction and sequencing was next performed by the Center for Functional Genomics at SUNY Albany.