GSM8328293: ERCC.NextSeq.b3.2; synthetic construct; RNA-Seq
1% dilution of ERCC RNA Spike-In Mix (4456740, ThermoFisher) Sample was processed into a short read library using the TruSeq Stranded mRNA Sample Prep Kit from Illumina, Inc (Cat#: 20020594). The fragmented mRNA was reverse transcribed to cDNA using random primers. This was followed by second stranded cDNA synthesis. The library preparation processes of mono-adenylation, adapter ligation and amplification were performed following the manual provided by the manufacturer. The quantity and quality of the libraries were assessed in a manner similar to that described for whole genome DNA library preparation. The procedure of 150 cycle paired-end sequencing on the Illumina NextSeq550 followed per the manufacturer's guidelines.