GSM6806329: Cyfip mutant heads at ZT16, sample 3 [ZT16HET3]; Drosophila melanogaster; RNA-Seq
Fly heads were quickly collected at the specific time points using liquid nitrogen. Total RNA was isolated using Trizol Reagent (Gibco) according to the manufacturer's instructions to extract total RNA. RNA-seq libraries were prepared using 500 ng of total RNA and the Illumina TruSeq Stranded mRNA reagents (Illumina; San Diego, California, USA) on a Sciclone liquid handling robot (PerkinElmer; Waltham, Massachusetts, USA) using a PerkinElmer-developed automated script.