Whole genome sequencing of Ascaris: 61h embryos
60h embryos irradiated with 200Gy X-ray followed by 1h recovery time (harvest at 61h). Embryo membrane was disrupted by 5x strokes in a metal dounce. DNA was isolated following Qiagen Genomic DNA Handbook, following the cultured cells protocol. Qiagen genomic tips 20/G were used to isolate genomic DNA. Libraries were prepared with Illumina Nextera Library Preperation Kit