GSM7032599: MG63, 1g, rep3; Homo sapiens; RNA-Seq
RNA was harvested using High Pure RNA Isolation Kit (Roche) as per the manufacturer's instructions. The quantity, the quality and the integrity of RNA was assessed using Agilent 2100 Bioanalyzer (Agilent). Total RNA was purified, fragmented and a double-stranded cDNA library was synthesized using the SMARTer Stranded RNA-Seq Kit (Takara) following manufacturer's protocols.