GSM7952133: C2C12 cells, lenvatinib-untreated-1; Mus musculus; RNA-Seq
Total RNA was extracted from cultured cells using RNeasy Mini Kit (Qiagen, Valencia, CA). Firstly, reference genome sequences and gene model annotation files of relative species were downloaded from genome website, such as UCSC, NCBI, ENSEMBL. Secondly, Hisat2 (v2.0.1) was used to index reference genome sequence. Finally, clean data were aligned to reference genome via software Hisat2 (v2.0.1).