Amplicon sequencing of M2 in Arabidopsis thaliana Combinatorial NLS 18849_B10
A single plant was used for extraction of genomic DNA according to the Berendzen method. iProof was used to amplify the target region and add barcodes to the amplicons to pool PCR samples. Up to 96 samples were pooled. The PCR pools were cleaned with Zymo-Spin II columns (Zymo Research). Cleaned PCR pools were send for next-generation sequencing of the target gene. All read data were demultiplexed, barcodes and adapter sequences were removed, and reads were trimmed and merged.