GSM7854350: Sir3_wt2_mRNA_Fig6_90min_after_release; Saccharomyces cerevisiae; RNA-Seq
Cells were resuspended in Trizol, cell walls were broken with bead beating and total RNA was extracted and DNAseI treated. Extracted total RNA amounts were measured with  the Qubit fluorimeter and the Nanodrop spectrophotometer and the quality was checked by a Bioanalyzer scan (Agilent). Each S.cerevisiae total RNA extract was mixed with the S.pombe total RNA extract at a mass ratio of 10:1. total RNA was used to make stranded Illumina RNA_seq libraries with the Illumina  TruSeq Stranded mRNA kit or the Illumina Stranded mRNA Prep Ligation kit .