GSM7854139: mouse ZFTA-RELA ependymoma tumor, ChIP-H3K27me3, rep1; Mus musculus; ChIP-Seq
Tissue was dissociated, crosslinked with 1.1% formaldehyde for 10 min, quenched and frozen. After thawing, cells were lysed, followed by nuclear lysis. Lysates were sonicated using a Diagenode Bioruptor. ChIP-seq libraries were prepared using the TruSeq ChIP Library Preparation Kit (Illumina, IP-202-1012). 10 ng of DNA was used as starting material. Libraries ranging from 250-350 bp were extracted from agarose gel incisions and PCR amplified for 18 cycles. Libraries were purified using AMPure XP beads and quality assessed using a Standard Sensivity NGS Fragment Analysis Kit.