HaCaT cells treated with Hibiscus sabdariffa extract from callus biological replicate 3
Total RNA was isolated from cells using TRIzol reagent (Invitrogen, Waltham, Massachusetts, USA) following the manufacturer's protocol. The quality of the extracted total RNA was assessed using the Bioanalyzer 2100 (Agilent, Santa Clara, CA, USA). Only total RNA with an RNA Integrity Number (RIN) value greater than or equal to 7 was utilized for library preparation. RNA sequencing libraries were generated using the TruSeq Stranded mRNA LT Sample Prep Kit, following the manufacturer's instructions (Illumina, San Diego, CA, USA). Subsequently, a total of 15 libraries, each with their respective indices, were subjected to paired-end sequencing using the NovaSeq 6000 system (Illumina).