infection with N74D capsid mutant in the presence of CsA replicate 1
Infected genomic DNA was digested with NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs) and its associated protocol and then ligated to double-stranded asymmetric linkers with a 3-T overhang in the presence of ligation enhancer. Ligation-mediated PCR was done with ligated DNA to constitute integration libraries that were later used for Illumina 150 bp paired-end sequencing.