16S rRNA sequencing of high Arctic beach microbial communities
Enrichments with Arctic beach sediment, 0.001% diesel, and artificial seawater, incubations were for 18 days. DNA extractions were performed using a modified version of the hexadecyl trimethyl ammonium bromide method. 16S sequencing library preparation was carried out according to Illumina 16S Metagenomic Sequencing Library Preparation. The library was validated on TapeStation (Agilent) and pair end sequenced on Illumina MiSeq with 500 V2 cycle kit.