GSM7792051: m2 Young Rep4 ATAC; Mus musculus; ATAC-seq
Nuclei were extracted from whole liver and lysed using diluted 10x (LB): 100mM Tris.Hcl pH 7.5, 100mM NaCl, 30mM MgCl2, 1% Igepal CA-630 with 2% BSA and protease inhibitor diluted in PBS. Tagmentation reaction was for 30 minutes at 37C then DNA was isolated and 6-PCR cycles (72C 3m, 95C 30s, (95C for 10s, 63C for 30s, 72C for 1 min) X15, 72C for 3 min, 4C) with i5xx and i7xx primers followed by a gel size selection of 220-1000bp.