GSM7950693: SC_Multiplex_CMO_Rep_1; Mus musculus; RNA-Seq
At day 5 upon tumor rechallenge, mice were euthanized and tumors disected. Tumors were minced and incubated for 30 min in HBSS shacking at 37 °C with 0.5 mg/ml Collagenase IV  and 50 μg/ml DNAseI, squeezed through a 70 μm cell strainer and re-filtered through a 40 μm cell strainer. Cells from each biological replicate (6 per experimental group) were pooled in equal cell numbers in n=2 per experimental condition and independently labelled using Cell Multiplexing Oligos (CMOs), following the manufacturer's protocol. Cells were then antibody-stained and DAPI-CD45+CD3+CD90+ cells that were CD8+ or CD4+ were FACS sorted and equal numbers of cells per replicate were mixed. The mixed cell suspension was processed using the 10x Genomics 3′ v2 kit, as specified by the manufacturer's instructions