GSM8153514: Huh7_control_3 rep3; Homo sapiens; RNA-Seq
Cells were collected and total RNA of the cells were extracted by TRIzol reagent (Thermo Fisher) Agilent 2100 Bioanalyzer (ABI) was performed to assessed quantity and quality of the cDNA libraries. The Double-stranded PCR products were treated with thermal denaturation and cyclization reaction using the splint oligo sequence and the final library was generated. Phi29 (Thermo Fisher) was utilized for amplifying the single-stranded circular DNA and then a DNA nanoball was generated.