Nanopore DNA-Seq w;TM2/TM6 female flies
High Molecular Weight Genomic DNA was obtained from 60 non-virgin female adult flies. Flies were flash-frozen in liquid nitrogen. DNA was extracted using the Genomic Tips 100/G kit (Qiagen) and quantified by Qubit (Invitrogen). Size was determined by TapeStation Genomic DNA Screen Tape assay (Agilent). Library preparation was performed on 3-7 g of genomic DNA using the Oxford Nanopore Ligation Sequencing kit (SQK-LSK-110) and quantified by Qubit. Libraries were sequenced on SpotON Flow Cells (R9 version, FLO-MIN106D) on a MinION Mk1c instrument. Base calling was performed using Guppy (Oxford Nanopore, version 6.4.6). Base-called reads were filtered for quality (Q8).