GSM7822094: E31-S1, Bd infected, Colorado, Skin_rep4; Anaxyrus boreas boreas; RNA-Seq
Tissue was harvested in RNA-later and stored at -80C. RNA was isolated from liver using the RNeasy Plus Mini Kit and isolated RNA from ventral skin using the RNeasy Fibrous Tissue Mini Kit (Qiagen, Germantown, MD) and homogenized tissue using a Polytron PT1600E (Kinematica AG, Bohemia, NY) or liquid nitrogen and mortar and pestle following the kit protocols. RNA quality was quantified using a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA) and verified a minimum RIN>8 and quantity >1ug. Stranded sequence libraries were prepared from total RNA (KAPA Stranded mRNA-Seq kit, Kappa Biosystems) and fragmentation was performed to select for larger sized inserts.