GSM7437600: WGBS_HEK293_TNRC18_WT_rep2; Homo sapiens; Bisulfite-Seq
Genomic DNA was purified using PureLink Genomic DNA kit (Invitrogen). DNA bisulfite treatment was performed using an EZ DNA Methylation-Gold kit (Zymo Research #D5005) according to the manufacturer's instructions. The recovered bisulfite-converted single-stranded DNA was processed for library construction using an Accel-NGS@Methyl-seq DNA Library kit (Swift BioSciences #30024) per the manufacturer's instructions. DNA was then enriched using PCR with primers compatible with Illumina sequencing. The quantity and molecular size of the library were confirmed with a Qubit HS DNA assay (ThermoFisher) and a Tapestation 2200 system coupled with High Sensitivity D1000 ScreenTapes (Agilent).