GSM7950242: AORB_1; Homo sapiens; RNA-Seq
Organoids were dissociated in TrypLE to collect single cell suspension. A cell purification kit was to enrich live cells to improve cell viability. Purified cell suspension was dilluted according to PIPSeq recommendation. PIPSeq T2 v4.0 kit was used to capture cells, isolate mRNA, synthesize cDNA, and prepare library. The cDNA was synthesized using the kits included in PIPseq-T2-v4 kits (Fluent BioSciences). Library preparation kits were also inlcuded in the same PIPseq kits. Libraries with a passing QC score were then used for sequencing on a 100-cycle NovaSeq SP kit with read lengths as specified by Fluent BioSciences.