GSM8198590: H3K4me1_heart_HH24_galGal6_WT_Rep2; Gallus gallus; ChIP-Seq
Cells were fixed with 1% MeOH-free formaldehyde for 10min, quenched with glycine and lysed with lysis buffer (10mM Tris pH 8.0, 100mM NaCl, 1mM EDTA pH 8.0, 0.5mM EGTA, 0.1% Sodium deoxycholate, 0.5% N-lauroylsarcosine). DNA was sheared to 200-700bp using a Covaris E220. Antibodies were incubated overnight at 4C. Bound DNA was precipitated with protein G beads, cleaned up and tagmented with Tn5 for 5min at 37C. Reverse cross-linking was done with Proteinase K, and cleaned up using the MinElute Reaction Cleanup kit (Qiagen). Libraries were amplified with dual indexing Nextera primers, followed by purification and appropriate size-selection using AmPureXP beads ChIPmentation