GSM6806471: CTCF Cut&Run, CTCF KO WT naive, rep2; Mus musculus; OTHER
Naive P14 TCR transgenic, hCD2-Cre, Rosa26-lsl-GFP, Tbet/Runx3 wild-type or double floxed CD8+ T cells were adoptively transferred into CD45 disparate recipients followed by infection with LCMV Armstrong. On day 4 post infection, P14 cells were sort purified and used for CTCF CUT&RUN analysis in live cells. The DNA segments from CTCF Cut&Run were end-repaired and ligated to indexed Illumina adaptors, followed by amplification by PCR with a low number of cells (18-21 cycles). The resulting libraries were seqeunced with Illumina Hiseq-2000 platform.