PacBio HiFi Pyrocephalus_nanus Isla_Santa_Cruz
We prepared libraries using standard PacBio recommendations, but full protocol details for library prep can be found in Van Dam et al. (2021) and PacBio (2012). We confirmed large amounts of  high-molecular-weight DNA using FemtoPulse (Agilent, Santa Clara, CA). DNA was sent to the QB3 Genomics facility at the University of California Berkeley for HiFi library preparation and sequencing on a Pacific Biosciences Sequel II platform, sequencing two SMRT cells with HiFi version 2 chemistry.