GSM8253019: ChIP_H1_WT_TEAD1_R1; Homo sapiens; ChIP-Seq
Cells were crosslinked using 1% formaldehyde in the medium at room temperature for 10 min and the cross-linking reactions were quenched using glycine. Chromatin solutions were prepared by adding 1mL of lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris, pH 8.1) to the pellets and sonicated using Covaris M220. For H3K9me3 and H3K27ac ChIP, 20 μg of chromatin was incubated with 5 μg antibody and Dynabeads (Life Technologies, 11204D) overnight at 4 °C. After the incubation, the beads were collected and washed by Low Salt Immune Complex Wash Buffer( 0.1% SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl, pH 8.1, 150 mM NaCl), High Salt Immune Complex Wash Buffer( 0.1% SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl, pH 8.1, 500 mM NaCl), LiCl Immune Complex Wash Buffer (0.25 M LiCl, 1% IGEPAL-CA630, 1% deoxycholic acid, 1 mM EDTA, 10 mM Tris, pH 8.1) and TE buffer.