GSM6806480: HiC Teff rep2; Mus musculus; Hi-C
Naive P14 TCR transgenic CD8+ T cells were adoptively transferred into CD45 disparate recipients followed by infection with LCMV Armstrong. On day 8 post infection, KLRG1+IL-7Ra- P14 cells were sort purified as effector CD8 T cells, which were then crosslinked, digested with MboI restriction enzyme and underwent proximity ligation. The biotinylated DNA segments were captured, and ligated to the Illumina Paired End Adaptors, followed by amplification by PCR with a low number of cells (18-21 cycles). The resulting libraries were seqeunced with Illumina Hiseq-4000 platform.