Whole-genome sequencing of E. coli in batch culture
A single colony was grown overnight in 10 mL of Luria broth then DNA was extracted using the standard phenol/chloroform method. The library construction and sequencing were performed at the Next Generation Sequencing Platform of Universitat Bern in Bern, Switzerland. Library preparation was done using the Illumina DNA Prep kit and sequencing was done by Illumina NovaSeq6000 flow cell, 2 x 150 bp run on two lanes.