NEXTFLEX_Small_RNA-seq: Sample F4_Lumbar
Human DRGs were collected within 4 hrs of cross-clamp from neurologically dead organ donors, and immediately fresh frozen in dry ice. Tissue was lysed using QIAzol and miRNA was purified using Qiagen's miRNeasy Micro Kit. Libraries were prepared with NEXTFLEX® Small RNA-seq v3 Kits. Single end sequencing was done on the Illumina NextSeq550 platform.