Nanopore reads
Sample libraries were prepared using Oxford Nanopore Technologies (ONT) Native Barcoding Kit 24 V14 (SQK-NBD114.24) to manufacturers specifications. All samples were run on Nanopore R10.4.1 flow cells on either a MinION, Mk1B or GridION. ONTs Guppy (v6.4.6) was used to demultiplex samples [1]. Samples were demultiplexed using Guppys Super Accurate basecalling model (dna_r10.4.1_e8.2_400bps_modbases_5mc_cg_sup.cfg).