Exome sequencing of RWPE-1
End-repaired DNA fragments were dAtailed and ligated with the NEBNext adaptorNEB, Ipswich, MA, USA. Biotinylated library baits and magnetic beads were mixed with the barcoded library for targeted regions selection using the SureSelect Human All Exon V6 Kit(Agilent Technologies, Palo Alto, Calif.). The captured sequences were further amplified for 150bp paired-end sequencing in Illumina Novseq6000 system (Illumina, SanDiego, CA, USA).