Zea mays B73, primary and seminal root tip (0-1mm), Nuclear RNA-seq, biorep1
One tenth of the RNA sample was used as a nuclear steady state input control. First strand cDNA synthesis was performed with Superscript IV First Strand Synthesis System using random hexamer priming. Libraries were made using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (starting at step 1.4.1 because cDNAs were previously made) in combination with NEBNext Multiplex Oligos for Illumina, Unique Dual Index UMI Adaptors RNA Set 1. The protocol to target 200 nt inserts was followed and the adapter ligated libraries were amplified for 14 PCR cycles.