hDel-v3 Direct-capture Perturb-seq of chimpanzee iPS cells.
cDNA libraries from polyadenylated mRNAs and Feature Barcode-compatible sgRNAs were generated by following the 10x Genomics Chromium Next GEM Single Cell 3 Reagent Kits v3.1 (Dual Index) User Guide (CG000316 Rev D). cDNA libraries from mRNAs and sgRNAs were quantified using Agilent Bioanalyzer, pooled at a 4:1 molar ratio, and sequenced on Illumina NovaSeq 6000 (28x10x10x90).