Transcriptomic analysis of microglia and DC-like cells sorted using different reporter lines
Transcriptomic analysis of microglia and DC-like cells sorted using different reporter lines
FACS-sorting of brain leukocytes. Approximately 8,000 microglial cells (p2ry12+; cd45+ or mhc2dab+; cd45low, n=2 for each Tg) and 1,200 DC-like cells (mhc2dab+; cd45high, n=2) were sorted.  RNA Ovation Solo RNA-Seq System (NuGen-TECAN) with the SoLo Custom AnyDeplete Probe Mix (Zebrafish probe set)