GSM8198102: RNA_IFNgamma_24h_R2_2; Mus musculus; RNA-Seq
For RNA-seq, 300,000 to 500,000 cells were centrifuged for 5 minutes at 4 °C at 500 rcf, resuspended in 150 µl RLT buffer (from RNeasy kit (Qiagen cat. No. 74181)) with β-mercaptoethanol, vortexed for 1 minute, and frozen at -80 °C. RNA was extracted using the RNeasy kit (Qiagen cat. No. 74181) following the manufacturer's recommendations. RNA was eluted in two rounds, with 45 µl and 20 µl RNase-free water, respectively 5 µl eluted RNA was processed with the QuantSeq 3' mRNA-Seq library kit (Lexogen cat. No. 015.96) according to the manufacturer's recommendations, using 16 PCR cycles for the amplification.