single-cell RNA-seq (5') of human endotracheal aspirate: extremely premature female neonate
Endotracheal aspirate samples were collected within one hour of birth and cryopreserved from extremely premature neonates (Gestational Age < 28 weeks, birth weight < 1,250 g) following institutional review board–approved protocols after obtaining informed consent from parents. Single-cell RNA-seq libraries were generated using the 10X Chromium Single Cell 5ʹ GEM, Library & Gel Bead Kit v1.1.