Oxford Nanopore of M. javanica VW4
A sequencing library was prepared starting with 2g of gDNA using the ligation sequencing kit SQK-LSK109 (Oxford Nanopore Technologies, Oxford, UK) following instructions of the manufacturer with the exception of extended incubation times for DNA damage repair, end repair, ligation and bead elutions. 30 fmol of the final library was loaded on the PromethION flowcell R9.4.1 (Oxford Nanopore Technologies, Oxford, UK) and the data was collected for seventy-two hours.