GSM7033611: A2_pooled_Input; Homo sapiens; MeDIP-Seq
Frozen PBMCs were centrifuged twice at 600x g for 10 minutes at 20°C and washed with RPMI+ 20% FBS. The cell pellet was resuspended in PBS to achieve a final volume of 7,500 cells per 1µL of suspension. An equal volume of the MNase digestion buffer (0.02 U/µL MNase (Thermo Scientific, Cat. No: 88216) in 2x lysis buffer was added to the cell suspension and gently vortexed. The cell suspension was then incubated on ice for 20 minutes, followed by incubation at 55°C water bath for 10 minutes. The digestion reaction was terminated using 30 mM EGTA. Then the digested chromatin was protease k (Ambion™