GSM7993221: H751L.total.rep1; Caenorhabditis elegans; ncRNA-Seq
Total (“input”) lysates and “IP” samples were subjected to RNA preparation as described above. Purified RNA was subjected to gel-based size selection. NEXTflex Small RNA Library Prep kit v3 (PerkinElmer, cat# NOVA-5132) was used to prepare libraries according to the manufacturer's instructions, followed by size selection of final PCR products. Libraries were sequenced using the Illumina Nextseq500 platform at the Kansas State University Integrated Genomics Facility.