GSM8252321: microglia,GF_Sham,Day7_1; Mus musculus; RNA-Seq
Sorted microglia from peri-hematoma were collected in 2mL TRIzol reagent and stored at -80℃. Gene expression at the mRNA level was analyzed using the SMART-Seq 2 protocol. Specifically, the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech, Japan) was employed to prepare the low-input library as follows: 1) Total RNA of pg level was used to prepare the cDNA library; 2) cDNA was purified and size-selected within the 150-300 bp range; 3) A sequencing-ready library was prepared by adding i5/i7 adaptors using the PCR method; 4) Library quality was assessed using an Agilent 2100 Bioanalyzer to determine size and concentration; 5) Libraries were sequenced on an Illumina Novaseq™ 6000 platform using a 2 × 150 bp paired-end sequencing protocol.