Staphylococcus condimenti from a blood stream infection
Genomic DNA was extracted using the Blood and Cell Culture DNA Midi kit (both Qiagen, Germany). For ONT sequencing, 2 g DNA were fragmented to an average length of 8 kb using Covaris g-Tube, and subjected to library preparation using the Nanopore sequencing kit (SQN-NSK006). The library was sequenced on a MinION device using the NC_48Hr_Sequencing_Run_FLO-MIN103.py workflow of the MinKnown software according to manufacturers guidelines. Reads with an average read quality value below 5 according to metrichor cloud-based base calling (metrichor.com) were discarded.