MeRIP-seq of mus musculus: adult male liver
m5C RNA immunoprecipitation (IP) was performed with the GenSeq m5C RNA IP Kit (GenSeq, Shanghai, China) by following the manufacturers instructions. Both the input samples without IP and the m5C IP samples were used for RNA-seq library with NEBNext Ultra II Directional RNA Library Prep Kit (NEB, Massachusetts, USA). The library quality was evaluated with BioAnalyzer 2100 system (Agilent Technologies, California, USA). Library sequencing was performed on an illumina Novaseq 6000 instrument with 150 bp paired-end reads.