GSM7755591: Colon_DT_Cre-Negative_04; Mus musculus; RNA-Seq
Total RNA extraction was performed using Trizol reagent as of the manufacturers user guide (Thermo Fisher Scientific, Waltham, MA, USA). Samples were processed by Genewiz for PolyA purification and library preparation using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA).