Metagenomic sequencing of mus musculus: stool
PCR amplicons were purified with Vazyme VAHTSTM DNA Clean Beads and quantified using the Quant-iT PicoGreen dsDNA Assay Kit. After the individual quantification step, amplicons were pooled in equal amounts, and pair-end 2  250 bp sequencing was performed using the Illlumina MiSeq platform with MiSeq Reagent Kit v38.