GSM6415212: MCF7, IgG palmitate loaded with NAT10 siRNA_1; Homo sapiens; RIP-Seq
Cell lysates were Iped by ac4C and IgG antibodies and total RNA was extracted using TRI reagent (Ambion) acRIP-seq library construction was initially quantified using Qubit 2.0. Followed by the accurate library concentration quantified by q-PCR (library effective concentration >2nM) to ensure standard library quality.