GSM8153459: Erlotinib2; Homo sapiens; RNA-Seq
Total RNA (1 µg) was processed to prepare an mRNA sequencing library using the MGIEasy RNA Directional Library Prep kit (#1,000,006,386; MGI Tech Co., Ltd., China) according to the manufacturer's instruction. The library was quantified using the QauntiFluor® ssDNA System (E3190; Promega Corporation, WI, USA). Sequencing of the prepared DNA nanoballs was performed using the MGISeq platform (MGI Tech Co., Ltd., China) with 100 bp paired-end reads.