GSM7822276: nTreg floxed control 3; Mus musculus; RNA-Seq
RNA was prepared from sorted Treg cells using the RNeasy Micro kit (Qiagen), and quality was assessed using the Bioanalyzer RNA chip cDNA was generated using the SMARTseq v4 ultra low input RNA kit for sequencing, and libraries prepared from the cDNA using the Nextera XT DNA library preparation kit.