Homo sapiens feces
RNA was extracted from stool suspension samples using TRIzol and prepared for next-generation sequencing. Briefly, reverse transcription used random hexamers. Subsequent DNase treatment and cleanup were followed by second-strand synthesis before library preparation using Nextera XT reagents (Illumina) and sequencing using a NovaSeq 6000 (Illumina). Although originally described as a consensus-level sequencing methodology, the depth of coverage was such that deep sequencing analysis also could be carried out. Bioinformatics analysis of the data was completed using a pipeline previously described