GSM8326528: FGF replicate 1; Homo sapiens; RNA-Seq
Briefly, S7D14 cell clusters were dispersed with TrpLE. Single cells with more than 85% viability were adjusted to 1,000 cells per μL. Approximately 16,000 cells were added to each channel of a 10X loading chip and then approximately 10,000 cells were captured. Single-cell libraries were constructed using the Single Cell 3 Library & Gel Bead Kit v.3 according to the manufacturer's instructions (10X Genomics)120. Briefly, S7D14 cell clusters were dispersed with TrpLE. Single cells with more than 85% viability were adjusted to 1,000 cells per μL. Approximately 16,000 cells were added to each channel of a 10X loading chip and then approximately 10,000 cells were captured. The captured cells were divided into Gel Beads in Emulsion (GEMs) and lysed, and the isolated RNA was reverse transcribed, barcoded, and amplified.