RNA-seq of Indigofera argentea roots: non-inoculated
Library preparation and Illumina sequencing were performed at Plateforme de Squenage Haut Dbit I2BC (Gif-sur-Yvette, France). Before library preparation, the quality of the RNA samples was assessed with an Agilent Bioanalyzer RNA 6000 pico chip, and the RNA concentrations were measured with a Qubit fluorometer. RNA library preparation was performed using Illumina Stranded mRNA Prep with ribosomal RNA depletion and PolyA purification. The libraries were sequenced on Illumina NextSeq 2000 to generate paired-end reads of 150x2 bases.