ITS2 region from soil sample
DNA extraction with DNeasy PowerSoil Kit. ITS2 region was amplified with the primers ITS3 (5-CAH CGA TGA AGA ACG YRG-3) and ITS4 (5-TCC TSC GCT TAT TGA TAT GC-3) as described in Tedersoo et al. 2014. Purified amplicons were sent to  Genome Quebec Innovation Center (Montreal, Canada) for barcoding using the Fluidigm Access Array technology and 300 base pairs paired-end sequencing running.