GSM6432623: HeLa_siYTHDF2_GLORI+_rep1; Homo sapiens; RNA-Seq
mRNA was purified by oligo (dT) beads. 100-200 ng of mRNA was used for GLORI treatment and construction of sequencing libraries. RNA libraries were prepared using modified eCLIP library construction, NEBNext Small RNA library kits (NEB) and SMARTer Stranded Total RNA-Seq kits (v3) - Pico Input mammalian (Takara) Library Prep Kit following manufacturer's protocols.