islet, SF1, rep 3
Total RNA extracted from Ad-SF1/Vector DIO murine islets with TRIzol reagent (15596018, Invitrogen) was evaluated using Agilent 2100 BioAnalyzer (Agilent Technologies) and Qubit Fluorometer (Invitrogen). Total RNA samples with RNA integrity number (RIN) > 7.0 and 28S:18S ratio > 1.8 were used in the subsequent generation and sequencing of RNA-seq libraries by CapitalBio Technology