16S rRNA amplicon seq: Enriched AL
DNA was extracted from the sample using the NucleoSpin Soil DNAExtraction kit (Macherey-Nagel, Germany) following the manufacturer's instructions. Then, PCR amplification targeting V3 and V4 hypervariable region were performed using 341F and 785R using the KAPA HiFi HotStart ReadyMix (2) (KAPABiosystems, United States).