GSM6807043: Liver, RD, H3K27me3, 2879; Mus musculus; ChIP-Seq
Liver and tumour tissues were pulverised before lysis on cell lysis buffer at 4ºC for 5-10 minutes. Nuclei were pelleted and resuspended in 1xPBS and the volume corresponding to 50-70 mg of tissue was used for chromatin extraction by adding nuclei lysis buffer at 4ºC for 5 minutes. Chromatin was sonicated on a Covaris E220 device (PIP: 105, Duty factor: 2, Cycles/burst: 200, Duration: 5 min). ChIP was performed with antibodies for rabbit anti-H3K27Ac (Abcam, ab4729) and rabbit anti-H3K27Me3 (Abcam, ab192985). ChIP-seq libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645)