GSM6413414: 6_T216_p637_LV_PG_RNA050721; Homo sapiens; RNA-Seq
RNA extraction using PureLink RNA Mini Kit (catalog # 12183025, Life Technologies), following manifacturer's protocol. cDNA synthesis using SuperScript® III First-Strand Syntesis SuperMix for qRT-PCR (cat. #11752-250, Life Technologies) NGS libraries were generated with the mRNA Stranded TruSeq protocol. Libraries were barcoded, pooled and sequenced on Illumina Nova-Seq 6000 sequencing system in paired-end reads mode, with reads 100nt long.