RNA-Seq of pooled juvenile Tanytarsus gracilentus
Extractions, library preparation, and sequencing were conducted by the University of WisconsinMadison Biotechnology Center, with sequencing on an Illumina NovaSeq 6000 (2 x 150bp reads on an S4 flow cell). This RNAseq library was generated from a pool of 155 first-instar juveniles. Samples were homogenized using a QIAGEN TissueLyser, then RNA was extracted using organic extractions followed by on-column DNase treatment with QIAGEN RNeasy Mini Kit. RNA quality and quantity were assessed using a NanoDrop (Thermo Fisher Scientific, Waltham, MA) and Agilent BioAnalyzer. Libraries were created using the Illumina TruSeq Stranded mRNA library preparation kit and were sequenced to obtain  100 million reads per sample.