ChIP-Seq of RPMI-8402 human cell line
Chromatin immunoprecipitation (ChIP) was performed as described previously (Panelli et al., 2022) with validated antibodies against BMAL1 (Cat. sc-48790, Santa Cruz Biotechnology), H3K27me3 (Cat. C15410069-50, Diagenode) and H3K27Ac (Cat. Ab4729, Abcam). ChIP-Seq libraries were prepared according to Accel-NGS 2S Plus DNA Library preparation kit (Cat. 21024, Swift Biosciences). ChIP DNA was quantified using a Qubit dsDNA HS Assay Kit (Cat. Q32851, ThermoFisher Scientific) and the quality was assessed by the 4200 TapeStation System using High Sensitivity D1000 Reagents (Cat. 5067-5585, Agilent Technologies). DNA sequencing was performed on NextSeq500 Illumina System using a Mid Output Kit v2.5 - 150 cycles flow cell (Cat. 20024904, Illumina) for reaching about 10 million reads/sample.