GSM8289676: DMSO_1; Homo sapiens; RNA-Seq
RNA samples from FB23-2 treatment and DMSO groups were isolated and purified using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer's procedure. Poly (A) RNA is purified using Dynabeads Oligo (dT)25-61005 (Thermo Fisher, CA, USA) using two rounds of purification. Then the poly(A) RNA was fragmented into small pieces using a magnesium RNA Fragmentation Module (NEB, cat. e6150, USA). Then the cleaved RNA fragments were reverse-transcribed to create the cDNA by SuperScript™ II Reverse Transcriptase (Invitrogen, cat. 1896649, USA), which was next to synthesize the cDNA library. At last, we performed the paired-end sequencing (PE150) on an Illumina Novaseq™ 6000 (LC-Bio Technology CO., Ltd., Hangzhou, China) following the vendor's recommended protocol. The differential gene expression was examined using a standard Illumina sequence analysis pipeline.