GSM8252677: mMA9_Gata2 KO_treat_H3K27ac; Mus musculus; ChIP-Seq
MA9 leukemia cells were collected from bone marrow of primary animals and transduced with Cre or MIT as control, then sorted for GFP and tdTomato. After 7-9 days in culture ChIP was performed. Briefly, samples were crosslinked, lysed, sonicated and immunoprecipated with the indicated antibody overnight. After a series of washes they were reverse crosslinked overnight and DNA extracted. Library prep was performed using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®, Illumina NovaSeq 6000, 2x300bp paired-end reads, multiplexed