Dm28c
Total RNA from the samples was isolated using RNAeasy kit (Qiagen), following the manufacturer's instructions. The RNA concentration and quality were analyzed by Agilent 2100 Bioanalyzer (Agilent Technologies), with the RNA 6000 Nano LabChip kit. The libraries were prepared with the TruSeq Stranded mRNA HT Sample Prep kit (Illumina) starting with 1.1 ug of RNA from each sample, following the manufacturer's instructions, which purifies the mRNA using magnetic oligo (dT) beads.