RNA-Seq of Actb and b-actin knockout mouse embryonic fibroblasts
RNA was quantified using a fluorimetric RiboGreen assay and RNA integrity was assessed using capillary electrophoresis to confirm samples had > 500ng RNA and an RNA integrity number (RIN) > 8.24 unique dual-indexed (UDI) TruSeq stranded mRNA libraries were generated using the Illumina TruSeq RNA Sample Preparation Kit according to manufacturers instructions.