GSM8197592: patient-specific GABAergic neurons, AAV-sh1500, Repl2; Homo sapiens; RNA-Seq
Total RNA was isolated using TRI Reagent (TR 118, Molecular Research Center, Cincinnati, OH) supplemented with BCP (B9673, Sigma-Adrich). Intact poly(A)+ RNA was extracted using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Ipswich, MA) Libraries were prepared using the Ultra II Directional RNA Library Prep Kit for Illumina and Unique Dual Index Primer Pairs (New England Biolabs)