sequences of bacteria
The Illumina sequencing method was used to extract genomic DNA from the sediment samples. After the extraction was completed, the extracted genomic DNA was detected by 0.8% agarose gel electrophoresis, and the DNA was quantitatively analyzed by UV spectrophotometer. PCR uses NEB Q5 DNA high-fidelity polymerase, and selects bacterial 16S rDNA V3-V4 region-specific primers. The forward primer is: F:ACTCCTACGGGCAGCA, and the reverse primer is: R:TCGGACTACHVGGGTWTCTAAT.  Library construction was performed using the TruSeq Nano DNA LT Library Prep Kit from Illumina. Qualified libraries were paired-ended at 2  250 bp using the MiSeq Reagent Kit V3 (600 cycles) on a 12 MiSeq machine