GSM7914541: DMSO-treated SG2 HCC4006 cells; Homo sapiens; RNA-Seq
For the experiment, 6 000 cells of each sample were loaded in Chromium device for emulsion-based production of single cell mRNA libraries with the 10X Genomics platform chemistry 3'V2 according to manufacturer's instructions. Reverse transcription was performed on the C1000 Touch Thermal Cycler, and cDNA were amplified for 12 cycles. cDNA quality control and dosage were performed on Fragment Analyzer, and then by PCR. single-cell RNA-seq 3' chemistry; Chromium Controller Single-Cell Instrument and Chromium Single Cell 3' Library & Gel Bead Kit (V2) were used to prepare individually barcoded single-cell RNA-Seq libraries. Double sided size selection of PCR products was performed with SPRIselect Reagent Kit (Beckman Coulter, France). The sequencing-ready library was cleaned up with SPRIselect beads and the sequencing was performed on a NextSeq 550 instrument (Illumina).