GSM6806989: hBJ,MNaseSeq,rep1; Homo sapiens; MNase-Seq
Cells were washed, permeabilized, and digested with 80U/mL MNase for 2 minutes at 37C. DNA from chromatin was purified, at which point, mononucleosome-sized DNA fragments were isolated using an AMPure XP bead selection. MNase-seq libraries were prepared using the NEBNect Ultra II for DNA Library Prep, following manufacturers protocols, with an input of 5-10 ng of purified DNA. The  size distribution of amplified fragments was assessed by TapeStation, and the concentration of libraries was determined by Qubit.