Hi-C sequecing of Graphium doson: one fifth instar  larvae whole body
The fresh fifth-instar larvae tissues were used to isolate cells, cross-link and then homogenized by tissue lysis, digested with the restriction enzyme DpnII overnight. Next, the cohesive ends were blunted, reversed and labelled with biotin-14-dATP. Subsequently, DNA was purified by removing biotin from unligated ends and sheared to a length of 200-300 bp via a Covaris M220 (Covaris, Woburn, USA). After biotinylated DNA fragments were captured by AMPure XP beads, point ligation junctions were pulled down by Dynabeads MyOneTM Streptavidin C1 (Thermofisher, Waltham, USA). Finally, High-quality Hi-C library was sequenced on the Illumina NovaSeq sequencing platform at Novogene