GSM7032546: lean_alpha, biological replicate 10 (RNA-seq); Mus musculus; RNA-Seq
Cells were collected in Trizol post FACS purification and isolated by a chloroform extraction, assisted by phase lock tubes. RNA was precipitated by isopropanol and cleaned up over an RNEasy microcolumn per the Qiagen's taking great care to avoid carry over of ethanol following the column washes. Elution volume was 30 microliter. Indexed sequencing libraries were constructed using the TruSeq RNA sample prep Kit v2 per Illumina instructions.