RNA-Seq of cibarium orgam from Aedes aegypti female adults
Oligo Dt beads were used to enrich mRNA with poly A tail. After, mRNa molecules were fragmented in small pieces and used to synthesized into first strand cDNA using random primers. The second strand cDNA was generated using dUTP instead of dTTP. The synthesized cDNA was subjected to end-repair and 3'adenylated. Adaptors were ligated to the ends of these 3' adenylated cDNA fragments. Finally, PCR amplification was performed. The library was amplified to make DNA nanoball and sequenced on DNBSEQ platform using 150 bp paired-end reads, stranded specific.