LIV_ma1
250 ng of total RNA per sample was used as input for library preparation using QuantSeq FWD 3mRNA Library Prep Kit (Lexogen) in combination with UMI Second Strand Synthesis Module for QuantSeq FWD and Lexogen i5 6 nt Unique Dual Indexing Add-on Kit (Lexogen). Quality control for library quantity and size distribution was done using QuantiFluor dsDNA System (Promega) and High Sensitivity NGS Fragment Analysis Kit (Agilent Technologies). The final library pool was sequenced with NextSeq 500 (Illumina) using High Output Kit v2.5 75 Cycles (Illumina) in single-end mode, resulting in an average of 10 million reads per sample