RAD-seq of Xylosandrus crassiusculus XRA307-MIYO: adult female
DNA was extracted using Macherey-Nagel NucleoSpin Tissue kit. We performed Whole Genome Amplification using the Genomiphi kit V3. DNA was digested using the PstI-HF enzyme. Library were created by pooling specimens 19 by 19, and sonicated on a Covaris S220 to obtain 300-600 bp fragments. After sizing and purification, we performed PCR enrichment for each library to enrich fragment diversity. The pooled libraries were sent to MGX-Montpellier GenomiX, and sequenced on a SP lane in paired-end 2x150 nt mode on a Novaseq6000 (Illumina) according to the manufacturers instructions.