GSM6413463: Patient 1 Group: Exercise (Baseline) rep_3; Homo sapiens; RNA-Seq
Formalin‐fixed paraffin‐embedded (FFPE) normal colorectal mucosa specimens from participants for the usual and exercise groups with pre and post intervention) were used to perform GeoMx Digital Spatial Profiling (DSP). Each sample was scanned to generate a digital image. Region of interest (ROIs) were selected in the epithelium (2-6 per ROI per sample, size of each ROI between 27000-340000 mm2), including areas rich in crypts, at least 3 mm away of the edges, and avoiding tissue artifacts such as wrinkles and tears of the tissue. The selection of the ROIs was supervised by a trained pathologist The library was prepared by GeoMx Human Whole Transcriptome Atlas kit as the manufactures' manual.