GSM6806991: FS, donor2, scRNA-seq; Homo sapiens; RNA-Seq
Fresh human foreskin specimens were cut into 4 mm x 4 mm pieces. The fat layer was trimmed off from the bottom. Then the skin was floated on 2 mL of dispase in a 6-well plate and incubated at 37 °C for 1 hour. The epidermis was separated from the dermis and trypsinized for 12 minutes at 37 °C to get the epidermal single-cell suspension. For the dermis part, it was further cut into smaller pieces, then incubated with 0.25% collagenase I in 2 mL HBSS for 1 hour at 37 °C. Collagenase-treated pieces were trypsinized for 10 minutes at 37 °C. The tissue was then dissociated by pipetting and single-cell suspension was obtained. Epidermal and dermal cells were combined at a 1:1 ratio for loading onto the 10X Chromium Controller. Library was performed according to the manufacter's instructions (single cell 3' v3 protocol, 10x Genomics).