Helicobacter pylori DNA
The Allegro Kit is normally used for targeted sequencing. It includes a set of 192 Illumina i7 adaptors. DNA is fragmented and end-repaired by an enzyme mixture  then the Illumina i7 Adaptors are ligated to the ends. Up to this point we use the reagents from the Allegro Kit. The resulting DNA fragments contain only one type of adaptor on both ends, so this is not an amplifiable library. As background information, in the original Allegro protocol the second adaptor sequence (including i5 Index) is added by a target specific oligopool extension reaction. This is what they call SPET (single primer enrichment technology). The plexWell kit uses two rounds of transposase reactions, the first adds i7 indexed adaptors (96 different for 96 samples). After pooling up to 96 of these well-transposase reactions a second transposase reaction adds an i5 indexed adaptor to yield the final library, which is amplified in a standard PCR for 12 Cycles. From the plexWell kit we use only the second transposase that adds i5 indexed adaptors to activate the adaptor ligated Allegro fragments into an amplifiable library