GSM6656342: E3, B2-B9, 3; Mus musculus; RNA-Seq
Total RNA was extracted from cultures using the RNeasy Plus Micro Kit (Qiagen) following the manufacturer's recommended protocol. The aggregation cultured samples were homogenized before RNA extraction. The quantity and quality of total RNAs were checked by Nanodrop ratios of 260/280 and 260/310 and further checked with concentration, RIN, and 28S/18S ratio by Agilent 2100 at BGI Genomics. The 100-bp length paired-end reads from cultures were generated using BGISEQ-500 at BGI Genomics.