Nanopore sequencing of Anopheles gambiae genomic DNA
Genomic DNA from adult transheterozygous males harboring three transgenes (gZBD/+ ; Cas9/+ ; gFLE /+ ) was isolated using Blood & Cell Culture DNA Midi Kit (Qiagen) and sequencing library was prepared using Genomic DNA by Ligation library construction kit (SQK-LSK110, Oxford Nanopore).