GSM7991656: Control and trained cells, mixed sample (non-demultiplexed); Mus musculus; RNA-Seq
Two weeks post injection, mice were sacrificed with CO2, and spleens were isolated whole. Extracted spleens were mashed on 70um screens with 5mL FACs buffer (1% FBS, 5uM EDTA). Cells were pelleted, treated with RBS lysis buffer, and washed 2x with FACs buffer before sorting in a FACs Aria III. Cells were sorted into MARs-seq lysis plates containing barcoded primers and frozen in the -80C. Libraries were prepared according to the MARs-seq 2.0 protocol.