RNA-seq of Arabidopsis: germinating seeds
mRNA were enriched with Oligo (dT) magnetic beads, then broken into short segments. The first strand of cDNA was synthesized with six base random primers, then the double strand cDNA were synthesize and subsequently purified with AMPure XP beans. The purified double stranded cDNA was first end repaired, added with A tail and connected with the sequencing connector, and then AMPure XP beads was used to select the fragment size. Finally, PCR amplification was performed, and the PCR product was purified with AMPure XP beans to obtain the final library.