Shotgun sequencing of RCMV strain Maastricht from viral stock
Approximately 100 ng of DNA was sheared in an S220 focused-ultrasonicator (Covaris)  to fragments of approximately 450 bp. Sequencing libraries were produced by conducting seven PCR cycles using a KAPA LTP library preparation kit (Roche Sequencing and Life Science) with NEBNext multiplex oligos for Illumina (New England Biolabs). The libraries were analysed on NextSeq and MiSeq instruments (Illumina) to generate datasets consisting of 4,951,80845,770,652 paired-end reads of 150 or 300 nucleotides (nt).