RNA-Seq of Pinus sylvestris var. mongolica: inoculated for 4d
The mRNA is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA Seq sample preparation kit (Illumina, San Diego, CA, USA), the average insert size for the paired-end libraries was 300 bp (50 bp). And then we performed the paired-end sequencing on an Illumina Novaseq 6000 at the (LC Sciences, Houston, TX, USA) following the recommended protocol of the vendor.