16S rRNA of soil metageome: soil distal to Agaricus crocodilinus sporocarp
two step PCR  Nextera technology; Target enrichment: 16S rRNA - V4 (NEXTERA_515F, GTGYCAGCMGCCGCGGTAA; NEXTERA_806R, GGACTACNVGGGTWTCTAAT); MiSeq Reagent Kit v2 (500 cycles); PhiX Control Kit v3