GSM7855127: PIP-HDF-IRF4; Homo sapiens; ChIP-Seq
Cells were collected 2 days after viral transduction. Cultured cells were fixed in 11 % formaldehyde for 15 min at room temperature on a rotating platform. The formaldehyde was quenched by adding 125 nM glycine. The chromatin was sheared and immunoprecipitated with the antibody of interest. A control sample consisting of sonicated chromatin that was not immunoprecipitated was also sequenced. Illumina sequencing libraries were prepared from ChIP and Input DNAs by standard consecutive enzymatic steps of end-polishing, dA-addition, and adaptor ligation. Steps were performed on an automated system (Apollo 342, Wafergen Biosystems/Takara). After a final PCR amplification step, the resulting DNA libraries were quantified and sequenced on Illumina's NextSeq 500 (75 nt reads, single-end).