GSM7992602: Ctrl_2nd; Rattus norvegicus; RNA-Seq
Total RNA was isolated via TRIzol (Invitrogen, Carlsbad, CA, USA), followed by RQ1 DNase (Promega, Madison, WI, USA) treatment to remove DNA. The quantification and quality of the purified RNA were evaluated by using Nanodrop One (Thermo) to measure the absorbance at 260/280 nm (A260/A280). The libraries were subjected to 150 nt paired-end sequencing on an Illumina Novaseq 6000 system for high-throughput sequencing.