raw sequencing reads of MZUSP_25207_Dentispicotermes_sp
Whole genomic DNA extraction was performed using the DNeasy Blood & Tissue extraction kit (Qiagen). Library preparation was achieved using the NEBNext UltraTM II FS DNA Library Preparation Kit (New England Biolabs) and the Unique Dual Indexing Kit (New England Biolabs), with reagent volumes reduced to one-fifteenth of recommended volumes. The enzymatic fragmentation step was skipped for the preparation of libraries of samples preserved in ~80% ethanol at room temperature, as the DNA of these samples is typically highly fragmented. Libraries were pooled in equimolar concentration, and paired-end reads were generated using the NovaSeq 6000 and HiSeq X platform at a read length of 150 bp.