M_ah_9_22_ITS
The ITS1 region (ITS1FI2 and 5.8S primers) were sequencing.  The PCR reaction was performed using Q5 Hot Start High-Fidelity 2X Master Mix, reaction conditions according to the manufacturer's recommendations. Sequencing was performed on a MiSeq sequencer, paired-end (PE) technology, 2x300nt, using Illumina's v3 kit.