GSM6805813: RD_Bcat_cutrun; Mus musculus; OTHER
Organoid cells were enzymatically dissociated into single cells by incubation in prewarmed TrypLE at 37°C for 20min with pipetting disruption. CUT&RUN was performed based on published protocol (Skene et al, Nature Protocols 2018). Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit.