GSM7437264: SBC5, PARPi+4Gy-2; Homo sapiens; RNA-Seq
Total RNA was extracted by using High Pure RNA Isolation Kit (Roche Life Science, cat#11828665001) according to the manufacturer's instructions. RNA concentration was measured by NanoDrop 2000 UV-Vis spectrophotometer (Thermo Scientific). Total RNA-seq library was prepared from 1ug RNA with Illumina TruSeq stranded total RNA library kit (catalog 20020596) according to manufacturer's protocol. Library quality and yield were determined by Agilent TapeStation and Qubit fluorometer, respectively.