GSM8328331: Ndufa4KO_IFNg_Rep3; Mus musculus; RNA-Seq
For RNA-seq from wild-type, Ndufa4-/-, and Ndufa4l3-miR-147-/- (AKA Nmes1-deficient) BMDMs, total RNA was isolated from stimulated BMDMs using the PureLink RNA Mini Kit (Invitrogen) according to the manufacturer's instructions. Isolated total RNA was quantified using a Nanodrop 8000 Spectrophotometer.  RNA integrity numbers were determined using a TapeStation instrument (Agilent), and all samples used for RNA-seq library preparation had RIN numbers greater than 9. Three technical replicates were generated and sequenced for each genotype The RNA libraries were generated and sequenced externally by Genewiz/Azenta standard RNA sequencing protocols. RNA libraries were prepared for sequencing using standard Illumina protocols.