PacBio HiFi Sequencing of Corylus avellana 'Jefferson'
High molecular weight genomic DNA (gDNA) was extracted from 1g of flash-frozen leaves using Circulomics Nanobind Plant Nuclei Big DNA kit (Part No. NB-900-801-001 PacBio, USA) following the Nuclei Isolation TissueRuptor protocol (Part No. 9002755, Qiagen, Germany).Extracted gDNA were all sheared to 20kb with MegaRuptor 2 (Cat no. B06010002, Diagenode, Belgium). Standard HiFi libraries were prepped with SMRTbell Express Template Prep Kit 2.0 and SMRTbell Enzyme Clean Up Kit 2.0 at 1x reaction scale (5 ug sheared gDNA input per sample), including overnight ligation with non-barcoded SMRT bell adapters. Final HiFi libraries were size selected to omit fragments 10-12kb using Sage Science BluePippin (BLU0001, Sage Science, USA).