RNA-seq of one fungus
RNA extractions were conducted right after harvesting the samples, following the instructions of the RNeasy Kit (Qiagen, GmbH, Germany). RNA quality was checked using agarose gel electrophoresis (for physical observation) and spectrophotometry using the absorbance ratios 260/280 nm. Poly-A tail enrichment was used for fungal RNA using NEBNext Poly(A) Magnetic Isolation Module (New England Biolabs, Frankfurt am Main, Germany) and rRNA depletion for bacterial RNA following the Pan-Pro Ribo-Pool protocol. Sequencing on Illumina NextSeq500/550 (2x150bp) was performed at LGC Genomics (Berlin, Germany).