GSM7434719: BT67_H4K5ac_Input_2; Homo sapiens; ChIP-Seq
The crosslinked cells were lysed in hypotonic cell lysis buffer and nuclei were pelleted and lysed using 10% SDS (0.3% final concentration) and sonicated to achieve the chromatin size between 200-1000bp. ChIP-seq libraries were prepared using NEB II ultra, w/o shearing kit.