DNA-seq of water sample: Weizhou island
To achieve DNA amplification, 4 L 5 FastPfu buffer, 2 L 2.5mmolL1 dNTPs, 0.8 L 5mmolL1 forward primer , 0.8 L 5mmolL1 reverse primer, and 0.4 L FastPfu polymerase, 0.2 L BSA and 10 ng template DNA were introduced into the reaction system (20 L), supplemented with ddH2O.