GSM8197821: Control larvae, 5 day + 24h, replicate 1; Hermetia illucens; RNA-Seq
Five replicates (of two pooled larvae each) were collected from control and AFB1-treatments, respectively at each time point and flash-frozen in liquid nitrogen. Total RNA was extracted with TriZol reagent and subjected to DNase treatment, protein purification and stored at -80 degree C. The quality of total RNA was evaluated using Agilent Bioanalyzer and DeNovix Bioanalyzer. Library preparation was performed at BaseClear B.V. (Leiden, Netherlands). Sequencing libraries were sequenced paired-ended (150 bp read-length) on an Illumina NovaSeq 6000 platform.