RNA-Seq reads from Y. tenuis ATCC 10573 grown under various in vitro conditions
Y. tenuis RNA-Seq data was collected to refine the genome assembly. Cells were grown under various conditions (YPD 6 h, YPD 18 h, SC Glucose 18 h, SC Glucose 48 h, SC Xylose 18 h, SC Xylose 48 h). One culture was collected for each growth condition. RNA was extracted using a hot phenol method and treated with DNAse. RNA-Seq libraries were prepared using the KAPA Stranded mRNA-Seq kit (Roche). The libraries were pooled, quantitated by PCR, and sequenced on one SP lane for 251 cycles from both ends of the fragment on a NovaSeq 6000. Paired-end reads of 250 nt were recorded. Fastq files were generated and demultiplexed with the bcl2fastq v2.20 conversion software (Illumina). Adaptors were trimmed from the 3' end of each read.