V3-V4 region of 16S rRNA genes from soil sample
DNA extraction with DNeasy PowerSoil Kit. V3-V4 region of the prokaryotic small-subunit (16S) rRNA gene was amplified with the primers 341F (CCT AYG GGD BGC WSC AG) and 806R (GGA CTA CNV GGG THT CTA AT) as described in Frey et al. (2016). Polymerase chain reaction (PCR) amplification with tailed primers on Veriti 96-Well Fast Thermal Cycler (Applied Biosystems, USA) following the protocol modified from Frey et al. (2016). Purified amplicons were sent to  Genome Quebec Innovation Center (Montreal, Canada) for barcoding using the Fluidigm Access Array technology and 250 base pairs paired-end sequencing running.