GSM7434951: SOW004_ZR751_8h9363_3; Homo sapiens; OTHER
In brief, bromouridine was added into live cell culture at treatment end points to a final concentration of 2 mM and incubated at 37 degree for 30 minutes. Total RNA was extracted with Trizol reagent (Invitrogen #15596-018) and BrU-RNA isolation, library preparation, sequencing and data analysis were performed as described previously (Paulsen et al., 2014).