Whole genome sequencing of genome-edited wheat line
Sequencing libraries were constructed from ~2 g of genomic DNA using the PCR-free Illumina protocol at the K-State Integrated Genomics Facility (IGF). The libraries were subjected to size selection using the Pippin Prep system (Sage Scientific) to enrich for 400-600 bp fragments. The 2 x 150-bp Illumina reads were generated for edited line 387-3-6.