GSM8197636: Microglia_AAVsgAnxa1_2; Mus musculus; RNA-Seq
Cell suspensions were stained extracellularly as described above with Live/dead stain  and Fc block, and finally with a mix of antibodies in FACS buffer for 30 min at 4°C. All antibodies were diluted 1:100 in FACS buffer. Prior to sorting, cell suspensions were filtered through tubes with 35µm cell strainer caps (Falcon, 352235). For RNA-seq analyses, cells were sorted directly into 100µL Picopure™ Extraction Buffer (Thermo Scientific, KIT0204), incubated 30 min at 42°C and then stored at -80°C until RNA isolation and sequencing was performed. Library was constructed using  Smart-Seq 2 protocols