GSM7773176: Bulk RNA-seq Rapamycin Replicate 2; Saccharomyces cerevisiae; RNA-Seq
RNA extracted with TRIZOL cDNA reverse transcribed with Maxima RT using sample barcode and UMI dT30VN priming and a template switch oligo. Whole transcriptomes amplified with Kapa HiFi. 3' end libraries created by tagmenting with Illumina Nextera XT and indexing PCR with a 3' end specific primer (i5) and a Nextera (i7) primer.