WGBS of Agrotis infusa
DNA isolation from a single pupa was performed as described by Evans et al. (doi.org/10.3896/IBRA.1.52.4.11). Bisulfite conversion was performed using the EZ DNA Methylation Kit (Zymo Research), as per the manufacturer's protocol. Libraries were prepared using the True Methyl kit (Illumina), as per the manufacturer's protocol.