RNA-Seq of Dictyostelium in hypoosmotic conditions
Total RNA from D. discoideum was extracted from 6 samples, three biological replicates per condition, using the Qiagen RNeasy mini kit. RNA concentration was measured using a NanoPhotometer spectrophotometer. Samples were submitted to The Applied Genomics Core (TAGC), University of Alberta. Libraries were constructed using the Illumina TruSeq RNA Library Preparation kit and run on an Illumina NextSeq 500 system