GSM6475242: c1c2_Blue_input_rep2; Arabidopsis thaliana; OTHER
Seedlings were homogenized in liquid nitrogen, and RNA was harvested using Trizol reagent. mRNA was purified using OligodT beads. mRNA was fragmented and isolated with m6A antibody. RNA libraries were prepared for sequencing using standard Illumina protocols. m6A-seq libraries were prepared using RNA Library Prep Kit (NEB, E7770). Libraries were sequenced on the Illumina Nova 6000 platform as single-end 150 bp reads.