16S seq of human stool
Stool swabs were placed in a vial containing a lysis and stabilization buffer that preserves the DNA for transport at ambient temperatures. Samples were lysed using bead beating, and DNA was extracted by a guanidine thiocyanate silica column-based purification method.  PCR amplification of the 16S rRNA gene was performed with primers targeting the V4 variable region (515F and 806R).  Sequencing was performed in a pair-end modality on the Illumina NextSeq 500 platform rendering 2 x 150 bp pair-end sequences.