bacterial and fungal microbiome of tarspot disease of maize based on rRNA sequences
Leaf discs were collected from triplicated samples in the field from 16 maize inbreds that varied in tarspot severity and stored at -80C.  DNA was extracted with the Synergy 2.0 Plant DNA Extraction Kit (OPS Diagnostics, Lebanon, NJ, USA) using a bead beater to homogenize the leaf tissue.  DNA was purified with spun silica columns and isopropanol precipitation. The V3, V4, and V5 hypervariable regions of prokaryotic 16S rDNA were amplified with nearly universal PCR primers.  Likewise, the eukaryotic 18S rDNA hypervariable regions V3, V4, V7, and V8, were amplified with relatively conserved primers.  The ITS1 and ITS2 regions were similarly amplified.  Purified PCR product was used to prepare and sequence libraries on an Illumina MiSeq. This file was converted from fasta format and therefore has no valid quality scores.