GSM7610503: B16, AC484, Replicate 3; Mus musculus; RNA-Seq
RNA was isolated from cell pellets using the QIAGEN RNEasy Mini kit according to the manufacturer's instructions. PolyA RNA was isolated using the NEBNext Poly(A) mRNA Magnetic Isolation Module First-strand Illumina-barcoded ibraries were constructed using the NEBNext Ultra II Directional RNA Kit. During the library construction Ilumina R2 primer sequence, P7 sequences and a sample index were added.