metagenomic seq of groundwater from well H52 incubated with 12C bicarbonate for 21 days, replicate A
DNA sizing, quantitation, integrity, and purity were determined using the Agilent 2100 Bioanalyzer system (Santa Clara, CA, USA). Library preparation was performed with a NEBNext Ultra II DNA Lib Prep Kit (New England Biolabs, Ipswich, MA, USA) as recommended by the manufacturer, followed by multiplexed sequencing on one flow cell of an Illumina NextSeq 500 system (300 cycles) to generate 150-bp paired-end reads.