DNA-Seq of pooled adult male Tanytarsus gracilentus
Extractions, library preparation, and sequencing were conducted by the University of WisconsinMadison Biotechnology Center, with sequencing on an Illumina NovaSeq 6000 (2 x 150bp reads on an S4 flow cell). DNA was extracted from a pool of 7 adult males using a QIAcube HT from QIAGEN (Hilden, Germany), and DNA concentration was verified using the Qubit dsDNA HS Assay Kit (Life Technologies, Grand Island, NY). The sample was then prepared according to the Celero PCR Workflow with Enzymatic Fragmentation (Tecan Genomics, Redwood City, CA). Quality and quantity of the finished library was assessed using an Agilent TapeStation (Agilent, Santa Clara, CA) and Qubit dsDNA HS Assay Kit, respectively. DNAseq libraries were sequenced at  100_ coverage.