Hi-C sequencing of Zacco platypus
Muscle tissues were excised from these frozen specimens for the extraction of high molecular weight (HMW) DNA using a conventional CTAB-based method. Muscle tissue was frozen and ground in liquid nitrogen for the construction of the Proximo Hi-C library, following the instructions in the Proximo Hi-C kit manual (Phase Genomics, Seattle, WA, USA). Sequencing of the Hi-C library was performed on an Illumina NovaSeq 6000 platform with a 2 x 150bp paired-end run configuration.