GSM8198577: Targeted Bisulfite-Seq PBMC_day0_s260; Homo sapiens; Bisulfite-Seq
the standard phenol-chloroform extraction method for blood samples For the TBS-seq library preparation, adapter ligation and dA-tailing were performed using the NEBNext Ultra II Library prep kit and custom pre-methylated adapters (IDT). Purified libraries were then hybridized to the biotinylated probes. Under the following conditions: 2 min at 98°C; 14 cycles of (98°C for 20 sec; 60°C for 30 sec; 72°C for 30 sec); 72°C for 5 minutes; hold at 4°C, PCR amplification was performed after bisulfite treatment on captured DNA using KAPA HiFi Uracil+. Library quality control was conducted on the high-sensitivity D1000 assay on a 2200 Agilent TapeStation. Targeted Bisulfite Sequencing Adapter sequences were removed using cutadapt (v2.10) for the demultiplexed FastQ files