GSM7993260: Biol Rep 2 – Ws; Arabidopsis thaliana; RNA-Seq
Total RNA was extracted from 8-10 dpg roots of Wt and atx1setm seedlings using TRIzol reagent (Invitrogen-Thermo Fisher Scientific, Waltham, MA, USA), according to the manufacturer's instructions. Library was constructed at BGI-Hong Kong, following the Illumina Library Preparation protocol. RNA-Seq was carried out on roots of the atx1setm and the Wt of 10 dpg plants (two biological replicates). Library preparation was done at BGI-Tech, Hong Kong. Moreover, 50 cycles of single-end sequencing were achieved on HiSeq 2000 (Illumina) platform.