TagMAP of genomic DNA from LAD6 clone, mCherry
we tagmented 100 ng of genomic DNA from clones with transposome (Tn5                 loaded with oligo adapters), followed by a linear amplification using a primer that                 annealed on the BRINP1 promoters or the end of either EGFP or mCherry genes. 5 l of                 linear amplified material was further amplified with two rounds of nested PCRs to                 introduce barcode and Illumina Adapters (PCR1 and PCR2)