GSM7434629: Aneurysmal subaracnoid hemorrhage late_SS1_2; Homo sapiens; miRNA-Seq
MiRNAs were extracted from the serum samples using QIAseq miRNA Library Kit (QIAGENÒ) The quality of the samples was ensured using Advanced Analytical Fragment Analyzer. Sample concentration was measured with Qubit Fluorometric Quantitation, Life Technologies. Library preparation was performed according to the library preparation protocol (QIAseq miRNA Library Kit (QIAGEN). Human Brain Total RNA AM7962 (ThermoFisher Scientific) was used as positive control. Sequencing run was performed using Illumina NovaSeq 6000 SP platform using single-end 75-bp reads. Raw sequencing quality was assessed using FastQC.