Primers from Zeale; plate c; PCR negative control (blank)
Guano stored in pure ethanol at -20C; DNA extraction with modified QIAamp Fast DNA Stool Mini Kit ; PCR with Zeale (DOI: 10.1111/j.1755-0998.2010.02920.x) primers ; Ilumina sequencing using 2x150 bp chemistry and targetting 100,000 reads per sample