GSM8198164: Spleen monocytes CD115+ PH70% 24 h 2; Mus musculus; RNA-Seq
The mouse mononuclear cell fraction was obtained from peripheral blood and spleen by gradient centrifugation. After that, the cells were subjected to immunomagnetic sorting on a manual MidiMACS™ Separator using LS Columns (Miltenyi Biotec, Bergisch Gladbach,Germany) and using Anti-CD115 MicroBeads magnetic microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) in accordance with the manufacturer's recommendations. For each sample, monocytes were obtained from 5 mice. DNBSEQ Eukaryotic Strand-specific mRNA library