GSM8326363: 7 day male CCI replicate 1; Mus musculus; RNA-Seq
3mm piece of Cortex & Hippocampus was cut, then split into upper & lower quandrants and the upper quadrant was placed into Collaganase IV Cocktail (10mg of DNAse, 32mg Collaganse IV, 20mg of Trypsin Soybean Inhibitor Worthington Chemicals), tissue was mechnically and enzymatically dissociated and strained into a single-cell suspension. Live cells were enriched in each sample, by removing the dead cells using Miltenyi Biotech dead cell removal kit, viability was determined and samples were loaded on a Next GEM G chip and placed into a 10x Genomics Chromium Controller RNA libraries were prepared using Chromium Single Cell 3' Reagent Kits User Guide from 10x Genomics. Briefly, RNA was isolated and reverse transcibed into cDNA. The cDNA was then amplified and constructed into a 10x Genomics library.