GSM7033747: MEF 4in1_Prox1_OE day14 rep2 [timecourse bulk]; Mus musculus; RNA-Seq
Cells were harvested at days 2, 7, 14, or 28 by the addition of TRIzol to the cultures (Invitrogen). RNA harvested in TRIzol (Invitrogen) was isolated using the RNA Miniprep kit (Zymo Research). For bulk RNA sequencing, libraries were prepared according to the dUTP protocol and paired-end sequencing (2x100bp) was performed on the NovaSeq 6000 platform (Illumina).