Nanopore sequence of Paenibacillus
Paenibacillus sp. RC67 was isolated using ISP2 medium in aerobic conditions. For gDNA extraction, RC67 was grown on 10% TSB liquid media at 30C in a shaking incubator at 150 rpm until an OD of 0.5 was reached. Cells were pelleted using centrifugation at 400 rpm for 15 minutes and genomic DNA was extracted from the cell pellet using CTAB method. The whole genome was sequenced by Oxford Nanopore sequencing at SeqCenter in Pittsburgh, PA using R9.4.1 flow cells.