Illumina HiSeq 4000 paired end sequencing; The AT-hook DNA binding protein, TashAT2 of Theileria annulata acts as an analogue of mammalian HMGA to tune the BoMac cell transcriptome
The AT-hook DNA binding protein, TashAT2 of Theileria annulata acts as an analogue of mammalian HMGA to tune the BoMac cell transcriptome
The SV40 transformed cell line (BoMac) derived from bovine macrophages. The 6 transfected cell lines were used to generate log phase cultures with cells harvested by centrifugation (X120 g for 5 minutes). RNA was isolated using Trizol followed by RNeasy column (Qiagen), including an on-column DNAse digestion step, using the suppliers protocol.  RNA-seq was performed at the Centre for Genomic Research, University of Liverpool.