GSM8326923: Serum exosome miRNA, PD, 2021, 21; Homo sapiens; miRNA-Seq
Total Exosome RNA and Protein Isolation Kit (Thermo Fisher Scientific, Waltham, MA) was utilised to extract total RNA from serum exosomes, following the manufacturer's instructions. Small RNA library construction, alignment and mapping were performed by staff at Thermo Fisher Scientific. The small RNA libraries were prepared with the Ion Total RNA-Seq Kit version 2 on the Ion PGM System (Thermo Fisher Scientific, Waltham, MA). Quality control of the generated small RNA library was carried out using the Agilent High Sensitivity DNA kit on the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).