GSM8253034: heart, HET+cHIF1KO; Mus musculus; RNA-Seq
Total RNA was isolated with PeqGOLD total RNA isolation kit (Peqlab) according to manufacturers recommendations yielding a RIN of 9.5-10. Two rounds of mRNA purification were performed on total RNA (1 ug) using Dynabeads mRNA DIRECT Kit (Invitrogen). The Superscript III First-Strand Synthesis System (Invitrogen) was used to generate double-stranded cDNA. cDNA libraries were constructed using the Nextera XT DNA Library Preparation Kit (Illumina)