GSM7033720: Liver HCC tumour shProx1 endpoint rep2; Mus musculus; RNA-Seq
At humane endpoint euthanasia, primary mouse liver tumor samples were dissected and placed into TRIzol (Invitrogen). Samples were crushed and then homogenized using QIAshredder (Qiagen). For bulk RNA sequencing, libraries were prepared according to the dUTP protocol and paired-end sequencing (2x100bp) was performed on the NovaSeq 6000 platform (Illumina).