High-quality long-reads of the genome from swine-derived extraintestinal pathogenic Escherichia coli
The genomic DNA was extracted from all ExPEC isolates using a commercial DNA Kit (TIANGEN, Beijing, China). The quality and concentration of the bacterial genomic DNA were assessed through electrophoresis on a 1% agarose gel, and analysis was conducted using a NanoDrop2000 system (Thermo Scientific, Waltham, MA, USA) and a Qubit 4 Fluorometer (Thermo Scientific, Waltham, USA). The genome was sequenced through the MinION sequencer (Oxford Nanopore Technologies).