GSM8403610: FMNS826; female, mutant, NS; Mus musculus; RNA-Seq
Mice were sacrificed by cervical dislocation 24 hours after the last stressor. The mPFC was dissected from 1 mm coronal sections prepared using a mouse brain matrix (Stoelting Co., Wood Dale, IL), followed by extraction of total RNA using a GenElute™ Mammalian Total RNA Miniprep Kit and on-column DNase I treatment (Qiagen). The RNA integrity number and concentration were assessed using a Fragment Analyzer (Agilent 5400). Messenger RNA was purified from 400 ng of total RNA, and libraries were constructed using the NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina (New England Biolabs), following manufacturer's protocols. The libraries were sequenced on an Illumina NovaSeq 6000 system at a depth of 20 million 150-bp paired-end reads per sample.