GSM8197924: HiC_heart_HH22_galGal6_WT_Rep2; Gallus gallus; Hi-C
~1 million cells per replicate were fixed in 2% formaldehyde, lysed and digested with DpnII. Digested ends were marked with biotin-14-dATP. After reverse cross-linking, DNA was sheared to 300-600bp fragment size. Biotinylated junctions were enriched using Streptavidin T1 beads. Fragments were ends-repaired before adaptor ligation and indexing via PCR amplification. Purification and size selection were carried out using AMPure XP beads Modified in-situ Hi-C protocol (Melo, U. S. et al. 2020)