GSM7821552: OMCD of mice under High Salt Diet 2; Mus musculus; RNA-Seq
OMCD were microdissected from liberase-treated kidneys as previously described. All media were prepared and used in RNA-free environment. After dissection pools of 100-120 strutures from a single mouse were thoroughly rinsed and stored at -80°C on Lysis Buffer (Quiagen, RNeasy micro kit). RNAs were extracted according to the manufactured protocol. Libraries were prepared from the extracted RNA using the KAPA Stranded mRNA-Seq Kit from ROCHE