GSM6605083: F712 Oxycodone, low; Rattus norvegicus; RNA-Seq
Brain tissue was extracted and snap-frozen (at −30°C). Cryosections of approximately 500 microns (Bregma 2.28-0.72 mm) were used to dissect the nucleus accumbens core punches on a −20°C frozen stage. Punches from three sections were combined for each rat. using the Chromium Next GEM Single Cell Multiome Reagent Kit A (catalog number 1000282) following Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Kits User Guide (10X Genomics)