Total RNA-seq: Sample H5_L45
Extracted tissue was put in RNALater, stored at -80 C and shipped on dry ice to sequencing center.Tissue was lysed using QIAzol, RNA extracted using RNEasy Plus Universal , and subjected to Illumina ribosomal RNA depletion and total RNA Tru-seq Gold library preparation protocol. Single end sequencing was done on the Illumina NextSeq platform.