GSM8327071: Neurod1-Cre, lower small intestine, scRNA-seq; Mus musculus; RNA-Seq
Adult NeuroD1-Cre x EYFP mice were culled by cervical dislocation, GI tracts were removed and digested to single cell suspension, and sorted for EYFP fluorescence using an Influx Cell Sorter (BD Biosciences) at the Cambridge Institute of Medical Research (CIMR) Flow Cytometry Core Facility. DAPI and DRAQ5 staining, side scatter, forward scatter and pulse width gates were used to remove debris and cell clusters. cDNA library preparations were performed using the Chromium system (10X Genomics) by the Genomics Core Facilities at the Cancer Research UK (CRUK) Cambridge Institute (for the USI, LSI and LI) and the Wellcome-MRC Cambridge Stem Cell Institute (for the stomach and caecum).