HIFI CCS seq of Kmeria septentrionalis: adult leaf
Approximately 50 ng of qualified DNA was used for whole-genome sequencing. The preparation of SMRTbell template libraries was carried out according to the manufacturer's guidelines (PacBio's protocol for low DNA input). Size selection and concentration of the library were validated using the Agilent 2100 Bioanalyzer (Agilent Technologies) to confirm the presence of correctly sized SMRTbell templates. The prepared SMRTbell library was sequenced on the PacBio Sequel II system using the recommended sequencing parameters for low input DNA. Sequencing primers and polymerase were attached according to the manufacturer's instructions to create the final complex for the SMRT sequencing. The PacBio Sequel II system utilizes the SMRT Cell 8M, which provides more sequencing data per run.