GSM7032570: spt-2 KO C, 20C, replicate 3; Caenorhabditis elegans; RNA-Seq
RNA was harvested using Trizol and treated with Turbo DNA-free kit (Invitrogen). RNA libraries for RNA-seq were prepared using the NEB Next Poly(A) mRNA Magnetic Isolation kit according to the manufacturer's protocols. 1ug of total RNA was used to make the libraries. Libraries were indexed with NEBNext Multiplex Oligos for Illumina.