GSM8218361: Kidney, DbpA_KO_plus_IR_2; Mus musculus; RNA-Seq
RNA was harvested using Rneasy mini plus kit(Quiagen), 1.3 µg of total RNA was used for the construction of sequencing libaries. Quality and integrity of total RNA was controlled on Agilent Technologies 2100 Bioanalyzer (Agilent Technologies; Waldbronn, Germany). The RNA sequencing library was generated from 200ng total RNA using Ribo-off ( Vazyme) for mRNA purification followed by NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England BioLabs) according to manufacture´s protocols. The libraries were sequenced on Illumina NovaSeq 6000 using NovaSeq 6000 S1 Reagent Kit (100 cycles, paired end run) with an average of 5 x107 reads per RNA sample.