GSM8328257: chimpanzee neurons, differentiated, C3624, 2-week (PIPseq); Pan troglodytes; OTHER
The iPSC-derived excitatory neurons were dissociated using Accutase for 20 minutes. The reaction was neutralized with mTeSR medium, and the cells were then centrifuged at 1,000 rpm for 5 minutes. The non-viable cells were removed using the Dead Cell Removal Kit (Miltenyi Biotec, 130-090-101). The pelleted cells were resuspended in Cell Suspension Buffer and processed according to the manufacturer's recommendations (Fluent BioSciences). Briefly, cells were transferred into pre-templated instant partitions (PIPs) for mRNA capture. The integrity and quality of the PIPs/cell emulsions were visually inspected to ensure they met the manufacturer's standards. The samples underwent the protocol of the PIPseq T2 3ʹ Single Cell Sequencing Kit, which includes mRNA isolation, synthesis of complementary DNA (cDNA), amplification of cDNA, library preparation, library pooling, and sequencing. PIP-seq