GSM8197760: withSec (plant Secretion)-1; Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10; RNA-Seq
For RNA extraction, cells were treated with 250 μl 20g/L lysozyme for 20 minutes at 30oC. RNA extraction was done with TRIzolTM reagent (InvitrogenTM according to the manufacturer's protocol, DNAse treatment was performed and rRNA was depleted TURBO DNA-free (Ambion) kit was used to remove DNA contamination, as manufacturer's protocol. rRNA was depeleted with Ribo-Zero rRNA Removal bacterial Kit (Illumina) The preparation of the libraries and sequencing were performed by the G-INCPM unit at the Weizmann Institute of Science. Libraries were prepared with G-INCPM mRNA-Seq protocol without polyA capture. Sequencing was performed by HISeq Illumina Sequencer using high output mode.