scRNA-Seq of tongue
Raw gene expression matrix data were generated using Celescope v1.9.0 (https://github.com/singleron-RD/CeleScope). Quality control and data filtering were performed using FASTQC (version 0.11.7) and Cutadapt (version 1.17). The reads were then aligned to the reference genome GRCh38 (Ensembl version 92 annotation) using STAR (version 2.6.1b). Gene expression matrices were generated using FeatureCounts (version 1.6.2).