GSM8198302: Pup1, scRNAseq; Mus musculus; RNA-Seq
Proximal tibia and distal femur epiphyses from postnatal day 5 mouse pups were collected and digested with Collagenase I tissue removal solution (1% Collagenase I, 2.5% trypsin w/o EDTA, 30mM Cacl2, in HBSS) at 37ºC for 45 minutes to remove muscle tissue that remained following dissection. Cartilage samples were then incubated at 37ºC in Collagenase II single cell isolation solution (1% Collagenase II, 2.5% trypsin w/o EDTA, 30mM Cacl2, in HBSS) for 2-3 hours with brief vortexing every 10-15 minutes. Single cells were passed through a 40um cell strainer in PBS containing 0.04% BSA. Library was performed according to the manufacter's instructions (BD Rhapsody HT Express Single Cell Analysis System).