GSM7993181: D130; Mus musculus; RNA-Seq
Bone marrow cells were extracted using a syringe, and residual bone fragments were enzymatically digested with collagenase. Following red blood cell lysis, the bone marrow cells were suspended in flow buffer (DPBS+2% FBS), filtered using a cell strainer, stained with markers (CD31, CD45, Ter119, CD51, PDGFRα) at 4℃ for 1 hr, and subsequently sorted using a BD FACSAria. Library was performed according to the manufacter's instructions (single cell 3' v3 protocol, 10x Genomics).