Nanopore sequencing of Anopheles gambiae genomic DNA
Genomic DNA from 16 adult Ifegenia transheterozygous males harboring both transgenes (Cas9/+ ; gFLE /+ ) was isolated using Blood & Cell Culture DNA Midi Kit (Qiagen) and sequencing library was prepared using Genomic DNA by Ligation library construction kit (SQK-LSK110, Oxford Nanopore).