Amplicon sequencing of 16S RNA gene of freshwater sediments
We amplified the V4 region of the 16S rRNA gene in two steps: first with the primers 515F (Parada et al., 2016) and 806R (Apprill et al., 2015), and second with the FlowCell primers. We pooled and purified PCR products using AxyPrep MagBead PCR Clean-Up (Axygen Biosciences, Union City, CA).