GSM7914421: GEX:eVAT Lean and eVAT Obese and Spleen Lean and Spleen Obese (exp3); Mus musculus; OTHER
Immunocytes were released by mechanical disruption followed by filtering and washed in RPMI containing 5% fetal calf serum (FCS). Red blood cells were lysed prior to filtering using ACK lysing buffer Cells were then purified by fluorescence-activated cell sorting. Single cells were then sent to the Broad Institute Genomics Platform, which performed encapsulation following 10x Genomics protocols. scRNA-seq and scTCR-seq; 10x Genomics library prep, 3' v3 chemistry or 5' v2 chemistry