RNA-sequencing of mus musculus p53/MCA induced sarcomes with radiation and CpG treatment
Tumors were dissected from mice, minced, and digested using the Miltenyi Biotec tumor dissociation kit. Tumor specimens and matched muscle control were harvested and stored in RNALater (Ambion) at 80C until all samples were collected. RNA extractions from each sample were performed using RNeasy Fibrous Tissue Mini Kit (Qiagen). Extracted total RNA quality and concentration were assessed on a NanoDrop Spectrophomoter (Thermo Fisher Scientific). RNA-seq libraries were prepared using TruSeq Small RNA Library Preparation Kits (Illumina) following the manufacturers protocol. RNA sequencing was performed on an Illumina Novoseq in 151 base pair, paired-end configuration. Greater than 50,000 reads per cell were collected per the manufacturer's recommendation.