GSM7335689: 7814-MH-3; Mus musculus; RNA-Seq
Microdissected Cortex and V-SVZ tissue samples from WT and JKO mice were dissociated using the Papain Dissociation System (see 'Primary microglia cultures'), and microglia were then isolated from the cell suspension by Magnetic Activated Cell Sorting using Cd11b microbeads per manufacturer instructions with MS columns and MiniMACs separator (Miltenyi Biotec, Cat # 130-093-634). RNA was harvested using Rneasy micro plus kit (Qiagen) RNA samples were submitted to Vanderbilt University Medical Center VANTAGE Core for poly-A enrichment-based library preparation and sequencing on the Illumina NovaSeq (PE-100).