WGBS of blood serum: adult meningioma
cfDNA samples isolated from serum were bisulfite converted using the Zymo EZ DNA methylation kit. Amplified libraries were analyzed using Agilent BioAnalyzer 2100 technology and were cleaned using 0.8X beads to remove residual primer-dimers. Libraries were pooled to a 4uM concentration and sequenced on an Illumina MiSeq v2 micro 300bp flow cell to confirm accurate representation of each library in the pool. The pool concentration was adjusted to 2uM and then sequenced on an Illumina NextSeq2000 P3 200bp flow cell.