Azrael100
Genomic DNA was fragmented using an enzymatic approach (NEBNext Ultra II FS kit). Resulting DNA fragments were size selected (>200 bp), using AMPure XP beads, the fragments were end repaired and Illumina specific adapter sequences were ligated to each fragment. Each sample was individually indexed, and a second size selection step was performed. Samples were then quantified, using a fluorometric method (Qubit), diluted to a standard concentration (4nM) and then sequenced on Illuminas NextSeq500 platform, using a NextSeq (300 cycle) kit.