GSM7684106: EWAT; scRNAseq; Mus musculus; RNA-Seq
All the mice used in this study were 10-week-old male C57BL/6J purchased from Slac Laboratory Animal Company (Shanghai, China). All mice were housed in an environment with controlled temperature and a 12:12 hour light/dark cycle. Before collecting WAT, the mice were anesthetized and infused with PBS heparin sodium solution (77mg/L) to remove blood from adipose tissue. Next, SWAT, NWAT, EWAT, HWAT, and MWAT were collected and the adjacent lymph nodes were removed from SWAT and MWAT. SWAT, EWAT, and NWAT from six mice were pooled for nuclear isolation and snRNA-seq experiments, MWAT from 10 mice for nuclear isolation and snRNA-seq experiments, and HWAT from 20 mice for nuclear isolation and snRNA-seq experiments. After nuclear separation, at least 8000 nuclei were immediately loaded onto the 10x Genomics Chromium controller (10X Genomics, PN110203). The libraries were prepared according to the manufacturer's instructions, using 10x Genomics single cell 3 'Library and Gel Bead Kit V3 (10x Genomics, 1000075) and Chromium Single Cell B Chip Kit (10x Genomics, 1000074).  The libraries were finally sequenced using the Illumina Novaseq 6000 sequencer at a depth of at least 20,000 reads per nucleus with a pair-end 150 bp (PE150) reading strategy (performed by CapitalBio Technology, Beijing). snRNAseq