GSM6806522: Passport, Waterlogged, Replicate 1; Hordeum vulgare; RNA-Seq
Roots of plants grown under control conditions or subjected to partial submergence for 24 hours were rinsed under running tap water, and frozen in liquid nitrogen. The roots of 3 plants were pooled prior to grinding for RNA extraction for each sample. The tissue was ground in liquid nitrogen and total RNA was extracted using Spectrum™ Plant Total RNA kit (Sigma-Aldrich). Following RNA extraction, RNA integrity was assessed using an Agilent 2100 Bioanalyzer (Agilent). All RNA samples had RIN values > 7.0. Libraries were prepared by BGI Genomics (Hong Kong)