RNA-seq of benign GGOs in Homo sapiens
Platelets RNA was isolated and reverse-transcribed to cDNA. 200 ng cDNA was used as input for amplification by the KAPA HiFi HotStart Ready Mix PCR Kit. For labeling of platelets cDNA for sequencing, all amplified platelets cDNA was subjected to nucleic acid shearing, and meanwhile labeled with Tn5 adapter using Tn5 Transposase. Subsequently, beads purified product processed by Tn5 Transposase was labeled a single index barcode using KAPA2G Robust PCR kit. All purified steps were carried out with AMPure XP beads. The final platelets RNA libraries were quantified by ABI 7500 real-time PCR system  and sequenced on Illumina NovaSeq using the 172 bp paired-end mode.