GSM7854494: CD14pos_KS010_2 (3423); Homo sapiens; RNA-Seq
Cells were freshly harvested from the culture before RNA collection. CD14+ and CD14- iMDSC samples were flow-sorted based on CD14 expression. LPS + ATP treated MDSCs, prepared as above, were first resuspended with 2-mercaptoethanol (Sigma-Aldrich: M6250) in lysis buffer (PureLink RNA Mini Kit, Invitrogen: 12183018A). Samples were frozen on dry ice until processing at Fate Therapeutics (San Diego, CA). RNA was collected, isolates quantified by fluorometric RiboGreen assay, integrity determined using capillary electrophoresis and samples converted to sequencing libraries using Takara Bio's SMARTer Stranded Total RNA-Seq–Pico Mammalian Kit v2 (634414). Samples converted to sequencing libraries using Takara Bio's SMARTer Stranded Total RNA-Seq–Pico Mammalian Kit v2 (634414).