GSM7330620: TCONV_A_5; Mus musculus; RNA-Seq
RNA was isolated by means of the RNeasy Micro kit following manufacturer's instructions (Qiagen). Per sample, an amount of 100pg or 2ng of total RNA was used as input for the SMART-Seq v4 PLUS protocol to generate full-length cDNA (version “011821”) from Takara Bio USA, Inc. Subsequently, 3ng of purified double-stranded cDNA is enzymatically fragmented and stem-loop adapters ligated. Libraries are then amplified and indexed, generating Illumina-compatible libraries with unique dual indexes (UDIs) according to the manufacturer's protocol. Sequence-libraries of each sample were finally equimolarly pooled and sequenced on NovaSeq6000 using the Xp workflow, v1.5 kits, single read (100-8-8-0) at the VIB Nucleomics Core (www.nucleomics.be).