16SrRNA Sediment Microbiome 1
Sediment was then collected from each octopus location in a sterile falcon tube. DNA extractions were performed on octopus sediment (0.25g) samples using the Qiagen DNeasy PowerSoil kit. Modifications included homogenizing coarse sediment for 45s at 2,500 RPM. The DNA samples were prepared for targeted sequencing (16S rRNA gene) with the Quick-16S NGS Library Prep Kit (Zymo Research, Irvine, CA). The primer set used was to target and amplify the V3-V4 region.