Tet neurons biological replicate one (Derived from Tet/Tet ESCs)
RNA sequencing libraries were prepared with Illumina-compatible NEBNextUltraIIDirectional RNA Library Prep Kit (New England BioLabs, MA, USA) at Genotypic Technology Pvt. Ltd.,Bangalore, India. 500 ng of total RNA was taken for mRNA isolation, fragmentation and priming. Fragmentedand primed mRNA was further subjected to first strand synthesis followed by second strandsynthesis.The double stranded cDNA was purified using NEBNext sample purification beads. PurifiedcDNAwasend-repaired, adenylated and ligated to Illumina adapters as per NEBNext UltraII Directional RNALibrary Prep protocol followed by second strand excision using USER enzyme at 37 C for 15mins.