LSU amplicon sequencing of source and biofloc water
DNA was extracted using the DNeasy PowerWater Kit (Qiagen, Valencia, CA, USA) according to manufacturers instructions. The fungal large-subunit rRNA (LSU) gene regions were amplified using LSU200A-F/LSU476A-R (Ascomycota) and LSU200-F/LSU481-R (all other fungi) primers to produce amplicons for Illumina sequencing via a two-step PCR. PCR1 amplified the target sequences and then PCR2 added barcodes and Illumina adapters. PCR products from individual samples were visualized, cleaned, quantified, and pooled for 2 x 300bp paired-end sequencing.