gene exon sequence
Primary cultureTCs were selected from fresh clinical tissues of genomic DNA samples of acceptable quality and randomly interrupted by an ultrasonic highperformance sample processing system (Covaris) into fragments with a major peak of approximately 200300bp. Subsequent DNA fragments were then endrepaired by adding an A base at the 3end and a library splice at both ends, and then, the library was captured and enriched with the exome chip. The amplification products were subjected to an Agilent 2100 bioanalyzer instrument (Agilent DNA 1004 Reagents) and QPCR quality control. We used the Illumina HiSeq family of platforms to perform highthroughput sequencing of each qualified library. The raw image data obtained from sequencing were converted into raw reads (raw reads) by Illumina Base Calling software, that is, doubleend reads (pairedend reads). The data were stored in FASTQ file format and called raw data.