GSM8153113: Heat shocked nucleoli, Rep3; Homo sapiens; OTHER
Trizol extraction Library prep was performed on total RNA by polyadenylating RNA (poly(A) polymerase tailing kit Lucigen), cDNA synthesis (Low input RNA synthesis and amplification kit, NEB). Samples were barcoded and prepared for sequencing using (SMRTbell Express Template Prep Kit and Barcoded Overhang Adapter A/B Kits)