GSM7329880: Srebp1a_ff_Myeloid_biol_rep_4; Mus musculus; RNA-Seq
Total RNA was extracted using Rneasy column (Qiagen) according to the manufacturer's protocol. Poly-A mRNA was extracted from total RNA by using an NEBNext Poly(A) mRNA magnetic Isolation module (NEB), after which RNA-seq libraries were prepared using a NEBNext UltraII RNA Library Prep kit for Illumina (NEB) according to the manufacturer's protocol.