Sym53_M4
Symbiotic Riptortus pedestris insects infected with C. insecticola were harvested in the fifth instar at 3 dpi. Insects were dissected and the M4 midgut region were harvested in RNAiso Plus (Takara Bio). RNA was extracted with the RNeasy mini kit (Qiagen). mRNA purification and fragmentation and the preparation of cDNA libraries were conducted using TruSeq RNA Library Prep Kit v2 kit (Illumina). The cDNA libraries were sequenced by Illumina HiSeq-2000.