GSM7792058: Muscle, rAAV, CTRL; Mus musculus; RNA-Seq
For single-cell RNA sequencing mononuclear cells of TA muscle were isolated and multinucleated muscle fibers were filtered out. Four TA muscles of each study group were pooled for the analysis. The tissue digestion and single-cell isolation process, including tissue mincing, collagenase digestion and filtration was conducted as previously described by us (K. A. Hemanthakumar, S. Fang, A. Anisimov, M. I. Mayranpaa, E. Mervaala, R. Kivela, Cardiovascular disease risk factors induce mesenchymal features and senescence in mouse cardiac endothelial cells. Elife 10, (2021).). Prior to scRNAseq, the cell viability was determined using an automated cell counter (Luna, Logos Biosystems). The Chromium Single Cell 3'RNAseq run and library preparation were done using the Chromium Next GEM Single Cell 3' Reagent Kit v3.1 chemistry according to the manufacter's instructions.