RNA-seq of Pisum sativum: treated leaf with Elice16Indures
Approximately 30 mg of plant tissues were homogenized with 100 l of TRI-Reagent (Zymo Research) using SILAMAT S5 vibrator (Ivoclar Vivadent, Schaan, Liechtenstein). Total RNA was extracted using Direct-zol RNA MiniPrep System (Zymo Research) and Gene Expression Profiling (GEx) library construction, QuantSeq 3 mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen GmbH, Wien, Austria) was practiced conforming protocol proposed by manufacturers, respectively. Pooled libraries were diluted to 1.8 pM for 1x86 bp single-end sequencing with 75-cycle High Output v2 Kit on the NextSeq 550 Sequencing System (Illumina, San Diego, CA, USA).