RNA-seq of OVCAR3 and CCL218 cancer cells with evolved hydroxychloroquine resistance
Cells were pooled, representing one-third of each 96-well plate, pelleted, and processed by Genewiz for RNA-seq and whole-exome sequencing (WES). Qiagen AllPrep DNA/RNA kits were used to isolate RNA and DNA from the same sample. Agilent SureSelect Human All Exon V8 kits were used and polyA selection performed for transcriptomes.