GSM7951582: SGFD1; Mus musculus; RNA-Seq
LT-HSCs were sorted from the BM of mice with control diet (CD) or  serine/glycine-free diet (SGFD)  for 3 months. RNA was extracted from sorted cells using a RNeasy Micro Kit (QIAGEN) following the manufacturer's introductions. The concentration and integrity of extracted RNA were evaluated by an Agilent 2100 Bioanalyzer with an Agilent RNA 6000 Nano Kit. Libraries for RNA-seq were constructed using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer's instructions.