GSM6413323: Ctrl_Inflammatory_Mo_scRNA-Seq; Mus musculus; RNA-Seq
Bone marrow cells were harvested from individual mice. Ly6C+CD115+ monocytes were then FACS sorted. The total RNA was isorated using RNeasy Mini Kit (Qiagen) The molecular-tagged RNA fragments were converted into cDNA followed by library establishment for next generation sequencing using Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (10x Genomics). The resulting libraries were quantified by Qubit dsDNA Assay (Thermo Fisher Scientific) and TapeStation D1000 (Agilent). The libraries were sequenced by Illumina HiSeq platform with the following configuration: read 1: 28 bp, read 2: 91 and i7 index: 8 bp, yielding approximately 400 M paired-end reads per sample