GSM7437855: HO mass, vehicle, Day5; Mus musculus; RNA-Seq
Ectopic tissue masses were harvested on day 5 from implantation site, minced and dissociated into single cell suspension by treatment with dispase (50 U/ml in Hanks' balanced salt solution) for 2 hours at 30 degrees Celsius. Cells were then suspended in 5% FBS/DMEM at a concentration of 700 to 1200 cells per microliter. cDNA libraries of cells from each group were generated by Chromium Controller, barcoded, and purified as described by the manufacturer and sequenced using a 2 x 150 paired-end configuration on an Illumina HiSeq platform at a sequencing depth of ~ 400 million reads.