GSM7743486: Lama3-UT1; Homo sapiens; OTHER
Genomic DNA was extracted using the ReliaPrep™ Blood gDNA Miniprep System kit (Promega) according to the manufacturer's protocol Library construction was essentially performed as described by Turchiano et. al (Cell Stem Cell, 2021). Briefly, gDNA was fragmented by enzymatic digestion using the NEBNext® Ultra™ II FS DNA Library Prep (NEB) to obtain average fragment lengths of 350 bp. The DNA was end repaired and a protruding 3'-A nucleotide was added according to the manufacturer's instructions  in order to enable the ligation of a linker sequence with a protruding 3'