GSM8030898: RNAseq_2i_d0_si_Tead4_rep2; Mus musculus; RNA-Seq
Total RNAs were extracted with TRIzol® reagent. For quantitative PCR, cDNAs were synthesized with HiScript® III RT SuperMix for qPCR (+gDNA wiper) (Vazyme Biotech). RNA-seq libraries were constructed using the VAHTS mRNA-seq V3 Library Prep Kit (Vazyme Biotech). The libraries were denatured and diluted at a proper concentration, then were sequenced on Illumina NovaSeq (Annoroad Gene Technology Co., Ltd).