RNA-seq of Rho(0) PC-3 cells grown in 5% O2 and then exposed to hypoxia
Library preparation, sequencing, and differential gene expression were performed by Novogene. Briefly, upon undergoing further quality control assessment, mRNA was purified using poly-T oligo-attached magnetic beads. Following cDNA synthesis from the mRNA template, end repair, A-tailing, and adapter ligation were performed. After size selection and PCR amplification, the library was checked with Qubit 2.0 and real-time PCR for quantification and Agilent 2100 Bioanalyzer for size distribution detection. Sequencing was then performed on the Illumina NovaSec 6000 platform with a paired- end 150 bp read length and a read depth of > 20 million reads per sample.