GSM6416142: scRNA-seq of SRF KO iPSCs; Mus musculus; RNA-Seq
Total RNA was extracted from cells for RNA-seq using TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc.). 10x Genomics Chromium platform was used to capture and barcode cells to generate single-cell Gel Beads-in-Emulsion (GEMs) by following the manufacturer's protocol. The resulting libraries were sequenced on an Illumina NovaSeq 6000 System.