GSM7612579: RAM13_Tumor; Homo sapiens; RNA-Seq
Single-cell suspensions were prepared from primary tissues using the gentleMACSTM tissue dissociator and tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) using the “soft tissue” setting. PBMC were processed using Lymphocyte Separation Medium (Corning Inc., Corning, NY) density gradient centrifugation or SepMate PBMC Isolation (StemCell Technologies, Vancouver, Canada). scRNA-seq libraries were constructed according to the manufacturer's protocol.  Pooled V(D)J, 5' GEX libraries were sequenced on a NovaSeq 6000 SP100 flowcell (Illumina, San Diego, CA) to obtain 5,000 reads/cell and 20,000 reads/cell depth, respectively.