GSM7884767: LPS-ChIP-MDM; Mus musculus; ATAC-seq
ATAC-seq was carried out following the process described by Daniel with minor modifications. Macrophages were isolated and counted to obtain 50 cells /ml in PBS. The nuclei of macrophages were isolated with ATAC-LB. After DNA fragmentation and purification, the DNA was amplified using 14 cycles. The fragment distribution of the libraries was assessed with an Agilent Bioanalyzer and libraries were sequenced on a HiSeq 2500 platform.