Illumina NovaSeq 6000 sequencing; RNA-seq of mouse wounds from different time points, treatment and genotype
RNA-seq of mouse wounds from different time points, treatment and genotype
Mice received intravenous injections of F8-SIP or F8-VEGF-C (50 µg) into the tail vein every other day from day one post-wounding. Mice were treated with three injections (end of the study on day 7) or five injections (day 10).  Sample collection method: Mice were euthanized, and the back skin was removed. 5 mm circular biopsy punches were used to excise the tissue, and it was immediately snap-frozen in liquid nitrogen and shortly stored at -80°C until all samples were collected. RNA was extracted using the miRNeasy mini Kit (Qiagen, Hilden, Germany). RNA quantity and quality were assessed using a Bioanalyzer (Agilent Technologies, Santa Clara, California, United States) and subsequently used for generating cDNA libraries.  miRNeasy mini Kit (Qiagen, Hilden, Germany) RNA quantity and quality were assessed using a Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) and subsequently used for generating cDNA libraries