HiC reads of Corylus americana accession 'Rush' leaf tissue
Nuclei were extracted from flash-frozen leaf tissue using a Sigma CelLyticTM PN Plant Nuclei Isolation/Extraction Kit.  Crosslinking was performed following low-input protocols from Arima Genomics.  Crosslinked nuclei were quantified using a Qubit dsDNA High Sensitivity kit (ThermoFisher Scientific), samples were sheared to 600bp, and library preparation was performed using a KAPA Hyper Prep kit.  The final library was assessed for quality using the Agilent Tapestion System with a D1000 kit, and paired-end, 250-bp paired-end sequences were generated using an Illumina NovaSeq 6000.