GSM7855387: DGY1657_RPF_rep02; Saccharomyces cerevisiae; OTHER
Cells were harvested following previously described methods (Spealman et al. 2016). Briefly, cycloheximide was added to the media to a final concentration of 100μg/L and incubated for 2 min, before being separated from the media using rapid vacuum filtration using Millipore 0.22μm filters. Cells were resuspended from filters using polysome lysis buffer with cycloheximide and then flash frozen immediately in liquid nitrogen before being transferred and stored at -80°C. Ribosome profiling was performed by TB-SEQ, Inc. (Palo Alto, CA)