Illumina sequencing of freshwater bacteria
The V3-V4 region of the 16S rRNA gene was amplified using the bacteria specific primers 341F (5'-CCT ACG GGN GGC WGC AG-3') and 785R (5'-GAC TAC HVG GGT ATC TAA KCC-3'). PCR amplification, Illumina MiSeq library preparation and sequencing using V3 chemistry and 2 x 300 bp paired-end reads was carried out by LGC Genomics GmbH (Berlin, Germany).