GSM8328353: Red_Flesh_Mesocarp_RepA_R201; Malus domestica; Bisulfite-Seq
Total DNA was extracted from fruit mesocarp according to the method detailed by CTAB protocol detailed by Porebski et al, 1997 The library construction method and sequencing process are carried out according to the following steps: 1. Take genome DNA samples for quality control; 2. Fragmented genome DNA to the mean size of 200-350bp; 3. Blunt-ending and 3'-end dAaddition; 4. Methlated adaptor ligation; 5. Bisulfite conversion with EZ DNA Methylation-Gold kit (ZYMO); 6. PCR amplification; 7. Library quality control; 8. Library circularization; 9. The library was amplified to make DNA nanoball (DNB); 10. Sequencing on DNBSEQ (DNBSEQ Technology) platform.