GSM6606990: C5_1; Medicago truncatula; RNA-Seq
For Medicago early post-germination seeds, samples of radicles and cotyledons were used separately for RNA extractions. Samples were named accordingly: desiccated radicles of 1mm germinating seeds were called R1 and R1P (when subjected to PEG treatment), desiccated radicles of 5 mm germinating seeds were called R5 and R5P (when subjected to PEG treatment). About 100 freshly harvested seeds in two biological replicates were used to extract RNA for each sample. All samples were ground using micropestles and liquid nitrogen and RNA was extracted using the NucleoSpin® RNA Plant and Fungi kit (Macherey-Nagel, Düren, Germany) with lysis buffer containing 1% of polyvinylpyrrolidone (PVP-40) followed by incubation at room temperature for 10 minutes. All samples with good qualities (260/280 and 260/230 absorbance ratio >1.8; RNA Integrity Number, RIN>7; 28S/18S>1.7) were sent to Beijing Genomics Institute (https://www.bgi.com) (Hong Kong) for library preparation and sequencing on BGISEQ-500 platform, generating an average of 24M reads of 50bp per sample (20M SE50).