GSM7822935: HCD - untreated #1; Klebsiella pneumoniae subsp. pneumoniae; RNA-Seq
RNA extraction using the Hot Phenol methode, follwowing DNase treatment and PCI extraction. RNA samples were fragmented using ultrasound, treated with T4 Polynucleotide Kinase, Terminator Exonuclease, poly(A) tailed and 5' triphosphate removed, adapter ligated to 5' ends and cDNAs were synthesized using oligo(dT) adapters. dRNA-seq (doi: 10.1093/nar/gky765)