GSM7105840: Embryonic meninges, E14.5, Foxc1 null, embryo 7; Mus musculus; RNA-Seq
Foxc1 null and wild-type embryos were harvested at E14.5 in cold PBS and dissected in RNALater solution (Thermo). RNA was isolated from meningeal tissue by RNeasy Mini Kit (Qiagen). Library construction and sequencing was performed at the BRC Sequencing Core (University of British Columbia, Vancouver, BC). Sample quality was checked using the Agilent 2100 Bioanalyzer to ensure high RNA integrity. Samples were then prepped following the standard protocol for the NEBnext Ultra II Stranded mRNA (New England Biolabs).