acRIP-Seq of 293T cells (IgG)
Polyadenylated RNAs were isolated from total RNAs by Oligo(dT)25 Dynabeads. Acetylated mRNAs were immunoprecipitated by rabbi- monoclonal-IgG-isotype-control-conjugated Dynabeads Protein A. Librarys were constructed by following the Smart-seq2 procedure.