GSM8365369: PL_1_2; Mus musculus; ATAC-seq
ATAC-sequencing libraries were generated using Nextera® DNA Sample Preparation Kit (Illumina, FC-121-1030) following methods previously described (Buenrostro et al., 2015, 2013). A total number of 80,000 microglia were pooled from two animals (40,000 cells from each) and collected in siliconized microcentrifuge tubes (Sigma, T3406-250EA) containing 300 µL medium A. For each group, at least two pools of two mice each were processed. Cells were pelleted by centrifugation (10 min, 4°C, 500×g), resuspended in 50 μL of cold lysis buffer (10 mM Tris–HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630) and immediately centrifuged as before. Next, nuclei were resuspended in 50 μL transposition reaction mix (1 × TD reaction buffer, 2.5 μ