Strand specific RNA-Seq of homo sapiens + EBV: child male established BL tumor cell line with latent EBV phenotype
EBV lytic vs latent phenotype was determined with FACS for the EBV glycoprotein gp350. Then, total RNA was isolated from BL cell lines using the Qiagen RNeasy kit with DNase digestion. Novogene prepared the RNA sequencing libraries, performed the sequencing on an Illumina Novaseq with 150 bp paired end reads, and did the initial bioinformatic analysis.