GSM7884530: pTOsk-Ez-KD.2 [sRNA]; Drosophila melanogaster; ncRNA-Seq
Regulatory small RNA extraction was performed as described in (Grentzinger et al. 2020).  Argonaute-small RNA complexes were isolated from 10 to 30 pairs of ovaries from 3-5-day-old flies using TraPR ion exchange spin columns (Lexogen, Catalog Nr.128.08). gel-free protocol without size selection - Library preparation using QIAseq-miRNA kit