RNAseq from immature leaf of Daphniphyllum macropodum (G)
Immature leaf tissue was harvested onto dry ice. DNA-depleted RNA was isolated using a Direct-Zol RNA miniprep kit (Zymo Research). RNA quantity and quality were assessed using the Agilent Tapestation system with RNA nano screentape. For short read sequencing, library preparation was performed from 200 ng high quality total RNA input using the NEBNext Ultra II Directional Library prep kit for Illumina in conjunction with the NEBNext Poly(A) mRNA Magnetic Isolation Module and unique dual index set (New England Biolabs), according to the manufacturers instructions. Libraries were pooled at equimolar ratios and sent for paired end 150 base sequencing at Novogene (UK) Company Ltd. on a NovaSeq 6000 instrument (Illumina), with 10 million reads per sample on average.