WGS of African swine fever: wild boar spleen
Purified DNA was quantified by Qubit DNA HS Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA) and then used for library preparation by Illumina DNA Prep, (M) Tagmentation (96 Samples) (Illumina Inc., San Diego, CA) according to the manufacturers protocol. Deep sequencing was performed on the NextSeq500 (Illumina Inc., San Diego, CA, USA) using the NextSeq 500/550 Mid Output Reagent Cartridge v2 (300 cycle) (Illumina Inc., San Diego, CA, USA) and standard 150 bp paired-end reads.