GSM7107731: SIR_mutpromoter_Sua7FLAG input rep1; Saccharomyces cerevisiae; ChIP-Seq
Cells were lysed by mechanical disruption in a bead beater. Chromatin was fragmented by sonication. IPs were performed using 5mL mouse monoclonal anti-FLAG® M2 antibody (MilliporeSigma, product no F1804) and 50uL DynaBeads Protein G magnetic beads (ThermoFisher Scientific catalog number 10003D).   DNA was purified using Qiagen spin column kit NEB Ultra II library prep kit