GSM8365322: Sample 1_VDJ; Mus musculus; RNA-Seq
The sorted cells were pooled and washed in the Laminar Wash Mini system (Curiox Biosystems) before loading to Chromium Controller and using the Chromium Next GEM Single Cell 5´ Kit v2 (10x Genomics). Libraries were created using the Library Construction Kit, 5´Feature Barcode Kit, Chromium Single Cell Mouse TCR Amplification Kit, Dual Index Kit TT Set A (PN-1000215) and Dual Index Kit TN Set A (PN-1000250) (10x Genomics) ).Their quality and quantity was assessed using the Agilent Tapestation system and the Qubit Fluorometer (Invitrogen), before being sent for sequencing to the SNP&SEQ Technology Platform, Science for Life Laboratory (Uppsala Biomedical Centre, Uppsala University, Sweden).