GSM7884544: 10x3_19_D007_lib; Homo sapiens; RNA-Seq
Samples included in this study were retinal tissues of 20 donors from the Utah Lions Eye Bank. All donors were screened for medical history, and only the ones with no of retinal diseases were used in this study. Post-mortem phenotyping with OCT were performed to confirm that there were no drusen, atrophy, or any other disease phenotypes on retina. One eye was collected from each donor. All eye tissues were collected and dissected within 6 hours post-mortem. With 4mm and 6mm disposable biopsy punches, macula and peripheral retina were collected and flash frozen in liquid nitrogen, and stored at -80°C before nuclei isolation.   Nuclei for snRNA-seq were isolated by fresh-made pre-chilled RNase-free lysis buffer. The frozen tissue was resuspended and triturated in lysis buffer and homogenized with a WheatonTM Dounce Tissue Grinder. Single cell Gene Expression Library was prepared according to Chromium Next GEM Single Cell  500 3' Reagent Kits v3.1 (10x Genomics).   Single cell ATAC Library was prepared according to Chromium Next GEM Single cell ATAC  511 Reagent kit v1.1 (10x Genomics).