THP-1, nomoxic, 1
Sequencing libraries were generated using QIAseq miRNA Library Kit and index codes were added to attribute sequences to each sample. Reverse transcription primers with unique molecular indices (UMIs) were introduced to analyze the quantification of miRNA expressions during complementary DNA (cDNA) synthesis and PCR amplification. At last, library quality was assessed on the Agilent Bioanalyzer 2100 and qPCR.