Human CD34+ cells treated with and without Interferon gamma
Total RNA was extracted from human CD34+ cells with and without IFN- (10 ng/ml) exposure for 48 hours (3 paired samples each) using an RNA extraction kit (Zymo) and quantified by nanodrop (Thermo Fisher). RNA integrity numbers (RIN) were determined for samples on a bioanalyzer (Agilent 2100) and samples were only used if RIN values above 7.0. Single-strand DNA circles and DNA nanoball production by rolling circle replication generated compatible libraries of sequences close to the 3 end of polyadenylated RNA.  Libraries were sequenced at 30M paired-end, 150 base pairs using the DNBSEQ platform system by BGI, USA.