ITS rRNA of bovine rumen for 96hr in-vitro comparison
DNA extractions were performed with 300uL of each fluid sample using the FastDNA SPIN Kit for Soil (MP Biomedicals, Solon, OH, USA) following manufacturers directions. Extracted DNA quantity and quality were evaluated using a nanodrop (Thermo Scientific Nanodrop 2000, ThermoFisher Scientific, Pleasanton, CA, USA), then DNA was stored at -20C until PCR amplification and sequencing.  Sequencing was done by the Environmental Sample Preparation and Sequencing Facility at Argonne National Laboratory (Lemont, IL, USA) according to their protocol using the Earth Microbiome Project primer set.