Total RNA sequencing of testis derived from 24-month-old ad libitum fed male mice
Total RNA from testis and sperm was extracted using the PureLink RNA Mini Kit and RNA quality was assessed on a 2100 Bioanalyzer using the RNA 6000 Nano Kit. Libraries were prepared using the Nextera DNA Library Preparation Kit and sequenced on an Illumina HiSeq 2000 instrument.