GSM6753338: MIAPaCa2_KRASG12R_RN118_untreated_8d_2; Homo sapiens; RNA-Seq
2x106 Dox-treated and untreated control cells were harvested, washed with PBS, lysed, treated with DNAse I (QIAGEN) and total RNA was extracted using the RNeasy Mini Kit (QIAGEN). Next generation sequencing libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit (Lexogen) according to the manufacturer's instructions.