GSM8155384: sn_rep21; Mus musculus; ATAC-seq
Initially, oocytes were cultured in M2 medium containing 2.5 μM milrinone and 5 μg/mL cytochalasin B for 15 minutes. The GV nucleus was precisely extracted with minimal cytoplasmic carryover, utilizing a Piezo impact-driven micromanipulator. After washing with 0.5% PVA/PBS, 50 nuclei were added to 50 μL of lysis buffer (100 mM Tris-HCl, 10 mM NaCl, 3 mM MgCl2, 0.5% NP-40 and 0.05% digitonin) for 10 minutes, and then subjected to tagmentation in TTBL mix (TD711, Vazyme) for 0.5 hours at 37°C. 5 μL of stop buffer was added to the reaction tube and incubated for 5 minutes at room temperature. DNA fragments were purified using ATAC DNA extract beads, underwent library amplification for an 18-cycle PCR, and subsequently, the libraries were purified again using 1.2× VAHTS DNA Clean Beads.