16JanS1
S. uncinata samples were collected every month in the at the moss bank in the Baton Peninsula on King George Island from Feb 2015~ Jan 2016. Three biological replicates were prepared for RNA extraction. The lower lignified parts were manually removed and upper green tissues were exclusively collected. Total RNA was extracted using an RNeasy Plant mini Kit (Qiagen). The integrity and concentration of RNA were evaluated using Bioanalyzer (RIN>6) and a Qubit RNA Broad-range Assay Kit (Life Technologies). To construct the libraries, 1 g of total RNA per sample was used for input for the TruSeq mRNA Library Kit