GSM7916190: WT 3; Mus musculus; RNA-Seq
RNA was extracted from iWAT of wild type, SOD1-G93A, Lep ob/+  and SOD1-G93A Lep ob/+  female mice at 90 days of age  using Qiazol followed by the mini Lipid tissue RNAeasy kit (Qiagen). Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample The clustering of the index-coded samples was performed on a cBot Cluster Generation System using PE Cluster Kit cBot-HS (Illumina) according to the manufacturer's instructions. After cluster generation, the library preparations were sequenced on an Illumina platform and paired-end reads were generated. Total number of reads was 30 million per sample.