18S rRNA metabarcoding of eukaryotic communities: Iron sludge from Lake Shoreline in Thielenbruch, Germany, 11/03/2022
Environmental DNA has been extraced using the Qiagen's PowerSoil Powerlyser Kit, and sent to sequencing facility at Dalhousie University (Halifax, Canada). The IMR sequenced the full 18S rRNA amplicon fragments using the Sequel II instrument (Pacific Biosciences) on an SMRT Cell 8M Tray.The details on librabry preparation, and sequencing are described on the IMR website: https://imr.bio/protocols.html.