GSM7695987: Trx Knockdown rep 3; Drosophila melanogaster; RNA-Seq
For each of the heart samples,50 one-week old adult fly hearts were dissected and collected into ice-cold TRIzol LS reagent. Total RNA extraction followed the manufacturer's instructions (Direct-zol RNA Microprep, Zymo Research). The quality and concentration of RNA were assessed using agarose gel electrophoresis, NanoDrop 8000 Spectrophotometer, and Agilent 2100. The preparation of RNA libraries was conducted by Novogene (Beijing, China) using standard Illumina protocols.