GSM7105957: KOLF-2 cells, PTCHD1_SNV, Day 24, biol rep 2; Homo sapiens; RNA-Seq
2 x 106 cells were taken for RNA extraction by RNeasy kit (QIAGEN, Australia) RNA integrity was determined on the Agilent 4200 Tapestation System with High Sensitivity RNA ScreenTape analysis. RNA sequencing was performed according to sure select strand-specific RNA library preparation for Illumina Multiplexed Sequencing paired end, 100bp, 30M read RNA sequencing on the NOVAseq 6000 platform (Illumina, USA) at Genomics WA (Perth, Australia).