GSM8290390: MTX,replicate 1,bulk RNA-seq; Homo sapiens; RNA-Seq
For scRNA-seq, Peripheral blood mononuclear cells (PBMCs) were collected through density gradient centrifugation according to the Histopaque-1077 procedure (Sigma-Aldrich Co. LLC). For bulk RNA-seq, total RNA of NK-92 cell line was extracted using the RNeasy micro-RNA isolation kit (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions. For scRNA-seq, Single-cell RNA-seq libraries were generated using the SeekOne® MM Single Cell 3' library preparation kit (SeekGene Catalog No. SO01V3.1). For bulk RNA-seq, RNA libraries were prepared using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® following manufacturer's protocols.