GSM7069895: WT, rep3; Mus musculus; RNA-Seq
We washed cells twice with PBS, then used TRIzol (Ambion) and RNeasy Plus Mini Kit (Qiagen) to extract RNA following the manufacturer's recommended protocol. We quantified the quality and quantity of the RNA samples with an Agilent Bioanalyzer or 4200 Tapestation. All RNA samples (n=6) were of high quality with RNA integrity numbers ≥ 9.3. We enriched our samples for mRNA by performing polyA selection according to the manufacturer's recommended protocol. Then, we prepared sequencing libraries with standard protocols and sequenced the samples on an Illumina NovaSeq 6000 at the McDonnell Genome Institute at Washington University School of Medicine in St. Louis.