GSM6415652: Library B - gate 2; Homo sapiens; OTHER
After cell sorting, the Kozak sequences from the subpopulations were PCR amplified. Genomic DNA was extracted from the subpopulations using the DNeasy Blood & Tissue Kit (Qiagen, #69504). The DNA from each population was loaded in PCR reactions with 400ng input each. A second PCR was performed for the ligation of standard Illumina adapters. The PCR products were purified with Ampure XP beads and mixed in equimolar ratios