RNA-SEQ OF HUMAN SKIN
DNA was extracted via KingFisher FLEX instrument (ThermoFisher Scientific, Inc., Waltham, Massachusetts) and using Zymo ZR-96 magbead kit (Zymo Research, Irvine, California) following manufacturers instructions. The extraction protocol was modified to include a mechanical lysis step with a Qiagen TissueLyser. V1-3 region of 16S rRNA gene was amplified for sequencing in two-step, independent reactions using HotStar Taq Master Mix Kit (Qiagen) with 28F-519R primers