GSM7156278: PBMCs,  AC, scRNAseq, A2; Homo sapiens; RNA-Seq
The PBMCs were immediately separated from the samples by a density gradient cen-trifugation making use of Ficoll-Paque Plus medium (GE healthcare). To remove erythrocytes, 2 ml GEXSCOPE ® red blood cell lysis buﬀer (Singleron) was added to the solution for 10 min at 25°C. The liquid solution was then centrifuged at 500g for 5 min, and the solution was suspended in phosphate buffered saline. Blood specimens were isolated by centrifuge at 400 g at 4°C for 5 min, and the supernatant was dis-carded. The PBMCs were centrifuged at 400 g at 4°C for 10 min, and the supernatant was discarded. To obtain a single-cell suspension, PBMCs were resuspended using phosphate buffered saline. Finally, the trypan blue staining method was used to stain the samples and evaluate cell viability under a microscope. We used GEXSCOPETM Single Cell RNA Library Kit  to barcode single cells, capture mRNA from isolated single cells, and generate cDNA libraries for scRNA-seq. Then, individual libraries were diluted to 4 ng/μl, and pooled for sequencing on a Nova-seq 6000 platform with 150 bp paired-end reads.