GSM6657728: Unpaired training, Dam-Creb, rep2_TI1; Drosophila melanogaster; OTHER
Extraction of genomic DNA from the fly heads (50–100 per sample), amplification of methylated fragments, DNA purification, and sonication was performed according to Marshall et al. (2016). After sonication, DamID adaptors were removed by digesting overnight at 37° C with 5 units of Sau3AI (R0169S; NEB). The sequencing libraries were prepared according to the Illumina TruSeq nano DNA library protocol. llumina TruSeq nano DNA