RNA-seq of E. coli and P. aeruginosa co-culture
Total RNA was isolated from cells pelleted from 1.5 to 3 mL culture with the Zymo Research Direct-zol RNA miniprep kit (R2053).  RNA was subject to bacterial rRNA depletion (Azenta), then sequenced at a read depth of 9-15 million 2 x 150 bp paired end reads per sample on an Illumina sequencer.