GSM8014148: EOSRC, tumor, sample 5; Homo sapiens; RNA-Seq
Total RNA from tissue samples was isolated using the TriZol reagent (Life Technologies, Grand Island, NY, USA) and RNeasy Kit (Qiagen, Valencia, CA, USA) following the manufacturer's protocol. Transcriptome sequencing was outsourced to Medgenome Labs Ltd, Bangalore, India. The libraries were prepared using Illumina TruSeq2 kit, following rRNA depletion and sequenced on HiSeq 2000 platform to generate 2x100 bp paired-end sequence reads.