GSM6807311: primary human oral keratinocytes_24_h_rep1; Homo sapiens; RNA-Seq
The cells were lysed with Trizol to obtain total RNA and verification for purity and integrity of total RNA was conducted RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols. Sample RNA was segmented, the segmented RNA was reverse-transcribed to generate double-stranded DNA, and the double-stranded DNA ends were processed for PCR amplification. The products amplified by PCR were terminologically denatured to form single strands, which were cycled and a single strand circular DNA library was established. High-throughput transcriptome sequencing was performed with Illumina Nova6000 sequencing platform for the DNA library, and the original data of transcriptome sequencing was finally obtained.