NextSeq 500 sequencing; PolyAdenylated RNA Fragmented (PARFA)-Seq of human cells expressing the vaccinia virus protein F14
PolyAdenylated RNA Fragmented (PARFA)-Seq of human cells expressing the vaccinia virus protein F14
Cell line maintained in DMEM.  All cells were treated with doxycycline to induce viral protein expression. Trizol PARFA-Seq