Human: ECM cultured cells:FM2 cultured cells: Organoids (4:1:1)
The samples were sequenced using illumina NovaSeq SP - 100 cycles. The library was prepared with the addition 9 bp of Unique Molecular Identifiers (UMIs). UMIs are short sequences or molecular "tags" added to the DNA fragments in a sample library. These tags are added before PCR amplification and can be used to reduce errors and quantitative bias introduced by the amplification.