DNA-Seq of Canis Lupus Familiaris
Sample concentrations are normalized to 2nM and loaded onto Illumina NovaSeq 6000 flow cells at a concentration that yields at least 700Gbp of passing filter data per lane.  Loading concentration for WGS libraries has been optimized to maximize both well occupancy and unique read output while limiting duplicates associated with patterned flow cell technology.  Samples are sequenced using 151 bp paired-end sequencing reads according to Illumina protocols. The 10bp indexes are read during additional sequencing reads that automatically follow the completion of read 1.  Data generated during sequencing runs are simultaneously transferred to the YCGA high-performance computing cluster. A positive control (prepared bacteriophage Phi X library) provided by Illumina is spiked into every lane at a concentration of 1% to monitor sequencing quality in real time.