GSM6753396: RS4;11 DMSO scRNAseq; Homo sapiens; RNA-Seq
Excess dead cells were depleted from samples using bead-based Dead Cell Removal Kit (catalog number 130-090-101, MACS miltenyi Biotech). Cells were mixed in 1:1 ratio with mouse cells (unrelated to this experiment) and loaded to the Chromium controller. scRNAseq: Cells were combined with reverse transcriptase Master Mix and partitioned into Gel Bead-In EMulsions (GEMs) using 10X GemCode Technology, where the poly-A transcripts are barcoded with an Illumina R1 sequence, a 16 bp 10X barcode and a 10 bp Unique Molecular Identifier (UMI). 11-12 cycles of PCR was used to amplify the cDNA.