GSM8328712: sgCtrl_3; Homo sapiens; RNA-Seq
Control and ASH1L-knockout PC-3M cells were lysed with TRIzol, and total RNAs were extracted using the RNeasy Mini Kit (Qiagen, 74104). Library preparation and sequencing were performed by ATGC next-generation sequencing core facility at MD Anderson, using a 2x75 bases paired-end protocol on a NextSeq500 instrument.