DUB-negative rMERS-CoV
RNA was isolated from virus-containing supernatants or lung 528 homogenates with the QIAamp Viral RNA Mini Kit (Qiagen). Total RNA was reverse transcribed to 529 cDNA using RevertAid H minus reverse transcriptase (Thermo Fisher Scientific) and random hexamers. 530 The PLpro domain (nucleotides 4435-5409 of the MERS-CoV genome was amplified by PCR using 531 Accuzyme DNA polymerase (Bioline) and after purification the PCR product was sequenced