RNA-seq of mouse oocyte: middle-age 3
The GV RNA was extracted (picopure). cDNA library was constructed by TruSeq Nano DNA LT Library Preparation Kit, FC-121-4001, Illumina, USA. cDNA was fragmented by dsDNA Fragmentase (NEB, M0348S, USA). Paired-end sequencing was performed on an Illumina Novaseq  6000