GSM6481328: enAsCas12f-TP53_2-rep2; Homo sapiens; OTHER
Genomic DNA was isolated using Purelink Genomic DNA Mini Kit (Thermo Fisher, K1820) 1 µg gDNA was applied to fragmentation, end-repair, A-tailing, adapter ligation, and dsODN-specific amplification, following the published GUIDE-seq protocol.