control_hay_2_Bacteria
Rumen content was collected from 2 ruminally fistulated Holstein cows and strained through 2 layers of surgical gauze. The rumen inoculum was a mixture of rumen fluid and artificial saliva at a 1:1 ratio. Batch culture was performed using a rumen inoculum containing hay, concentrate and 10 mM of organic acids. Samples were collected after 24 hours culture and using for microbial analysis. Culture was performed in quadruplicate.