GSM7068659: N2A MOV10 KO cells, MOV10 WT transfected replicate 3; Mus musculus; RNA-Seq
Total RNA was extracted using Trizol (Ambion) The RNAseq libraries were prepared with Illumina's 'TruSeq Stranded mRNAseq Sample Prep kit' (Illumina). The libraries were quantitated by qPCR and sequenced on one lane for 101 cycles from each end of the fragments on a NovaSeq 6000 using a NovaSeq SP reagent kit. Read 1 aligns to the ANTISENSE strand.