Low deep genome sequencing of Artemisia freyniana: mature leaves
Total genomic DNA was extracted from silica gel dried leaves or herbarium specimens using TIANGEN plant genomic DNA extraction kit (TIAN-GEN Biotech., Beijing, China) following the manufacturers protocol. Extracted total DNA from silica gel dried leaves were sheared into about 350-bp fragments to build 350-bp insert libraries and DNA from herbarium specimens was used to construct 150-bp insert libraries. The DNA libraries were constructed using NexteraXT DNA Library Preparation Kit (Illumina, Shanghai, China) and were sequenced on the Illumina HiSeq Xten platform (Illumina, Shanghai, China).