single-nuclei RNA-Seq: pooled samples of human entorhinal cortex, gene expression library replicate #1
Nuclei were isolated from postmortem flash-frozen human brain tissue. Frozen blocks of tissue were cryosectioned (50um) and cells were dissociated as described in the published paper. Library was constructed from isolated nuclei using 10X's CellPlex and v3.1 chemistry. Downstream demultiplexing was carried out by using freemuxlet to distinguish cells from each individual donor, identification of donor was done using the CMO barcodes in the barcode libraries