RNA-Seq of Oncorhynchus mykiss: head kidney
The transcriptome library of each sample was prepared starting from 500 ng of total RNA using the TruSeq Stranded mRNA Library Prep kit (Illumina Inc., San Diego, CA) following the companys protocols. Each library was run on an Agilent 2100 Bioanalyzer using Agilent High Sensitivity DNA kit to ensure the proper range of cDNA length distribution. Sequencing was performed on an Illumina NextSeq instrument with NextSeq 500/550 High Output Kit v2 (75 cycles) in single-read mode (Illumina, San Diego, CA, USA).