GSM8155006: Donor HSPC; Mus musculus; RNA-Seq
Bone marrow from the bilateral femur and tibia was collected; red blood cells were lysed and mature hematopoietic cells were depleted using magnetic bead-based lineage antibody cocktail.  Pre-selected cells were then flow sorted to collect CD45.1+/Lin-/Sca1+ cKithi (LSK) HSPCs. 30,000 cells were collected from each experimental condition, barcoded with TotalSeqB antibodies, and multiplexed for sequencing. Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1