Bisulfite-Seq of 5mC: HeLa cells
HeLa cells were treated with 1%DMSO and genomic DNA was purified. After end repair and A-tailing and adapter-ligation, DNA fragments was treated with bisulfite to convert the unmethylated cytosine to uracil. RRBS libraries were sequenced on an Illumina platform and 150 bp paired-end reads were mapped to the reference human genome sequence (NCBI 36.1 [hg19] assembly by TopHat (Version 2.0.6).