ATAC-seq of Homo sapiens: effector CD8+ T cells
The peripheral blood CD8+ T cells purified from heathy donors were stimulated with a-CD3/a-CD28 conjugated microbeads, and 50,000 live CD8+ T cells were sorted by flow cytometry immediately before library generation at day2. ATAC-seq libraries were prepared for the paired-end sequencing using Omni-ATAC protocol (Corces et al. Nature Methods 2017)