GSM8426935: Caki-1_sgCTR_rep1; Homo sapiens; ATAC-seq
In sgCTR and sgVHL Caki-1 cells, nuclei were removed from samples and resuspended in the Tn5 transposase reaction mix. For 30 min, the transposition process was incubated at 37°C. After transposition, equimolar Adapter 1 and Adapter 2 were introduced, and the library was amplified using PCR. Libraries were purified using DNA clean beads after the PCR process, and library quality was evaluated with Qubit. The library preparations were sequenced on an Illumina NovaSeq 6000 platform after cluster creation, yielding 150 bp paired-end reads.