GSM7035774: Compound 8 (8-73) 10 µM-treated Mycobacterium marinum replicate 1; Mycobacterium marinum; RNA-Seq
The RNA samples were isolated using the NucleoSpin RNA kit (Macherey-Nagel). The bacteria were lysed by bead beating with 0.1-zirconium beads in 500 μL buffer RA1 and 5 μL β-mercaptoethanol for 1 min. All subsequent steps followed the recommendations of the manufacturer. The rRNA was depleted and mRNA was enriched using RiboMinus prokaryotic kit (Invitrogen). Conducted by BaseClear (Leiden, Netherlands)