The Immunomodulatory Effect of Mesenchymal Stem Cell-Derived Exosomes in Peripheral Blood Mononuclear Cells of Patients with Allergic Rhinitis
LPS and MSC-Exos groups were compared and analyzed for cell sequencing. Trizol reagent kit (Invitrogen, USA) was used to extract the total RNA. Extracted mRNA was enriched by Oligo(dT) beads fragmented into short fragments and reversely transcribed into cDNA using a NEB Next Ultra RNA Library Prep Kit for Illumina (New England Biolabs, USA). cDNA library was constructed using PCR and size selection by agarose gel electrophoresis. Illumina Novaseq6000 was used by Shanghai Wancheng Biotechnology Co., Ltd to sequence the library. The reads obtained from the sequencing machines were aligned to the reference genome using HISAT2. 2.4.