Deep sequencing of SIVcpzMB897, Gen 4, Mouse 121, Week 11
Viral RNA was reverse-transcribed using SuperScript IV according to the manufacturers instructions. Two separate multiplexed primer pools containing overlapping regions between, but not within the pools, were designed utilizing the Primal Scheme software in order to generate overlapping 400 base pair amplicons that spanned the entire viral genome. The amplicons were prepped for Illumina-based deep sequencing using the Nextera XT DNA Library preparation kit and the manufacturers instructions.