16S amplicon sequencing of kelp associated communities: algae bank seawater
PCR reactions were performed with universal primers spanning the 16S rRNA gene V4 hypervariable region (515F:5GTGCCAGCMGCCGCGGTAA 806R: 5 GGACTACHVGGGTWTCTAAT3 (Apprill et al. 2015; Parada et al. 2016). Sequencing was performed at Genome Qubec (Canada).