Xdrop - Oxford Nanopore long read seqeuncing of HAP1 and HepG2 tRNA gene deletion clones
Xdrop - Oxford Nanopore long read seqeuncing of HAP1 and HepG2 tRNA gene deletion clones
Cells were harvest using trypsin-EDTA solution (Sigma). Trypsin was subsequently inactivated with a minimum of 3-fold surplus of culture medium. Cells were pelleted and washed once with 1xPBS. Genomic DNA of was extracted using Quick-DNA kit (Zymo Research) according to manufacturerâ€™s protocol. The genomic DNA was used to perform Xdrop. In short, the genomic DNA was compartmentalized into droplets and enriched for the target region. The target-enriched DNA molecules were further used for droplet multiple displacement amplification (dMDA). Afterwards, amplified DNA was treated by T7 Endonuclease I, followed by size selection, end-repair, barcoding, and adaptor ligation.