Amplicon(16S/ITS)-sequencing of citrus root microbiome
Samples of citrus fruit were collected in three orchard associated to three different citrus accession, and field sprayed with biological products and copper-treatments. DNA was extracted from each sample of citrus fruit DNA quality and quantity was determinated. 16S and ITS amplicon library preparation and sequencing were performed using amplified primers and methods of prokaryotic 16S rDNA V4 region and fungal ITS.