RNA-Seq of bacteria: tick gut
The metagenomic DNA was extracted using DNA extraction kits (DNeasy Tissue Kit, Qiagen). The purity of the extracted metagenomic DNA sample was analysed using NanoDrop (Thermo Fisher Scientific, USA) by determining the A260/280 ratio. The primers targeting the 16S V3-V4 region of the 16S rRNA gene were used to set up the primary amplicon. After the qualification using agarose gel and nano drops, the sample was processed for library preparation (Nextera XT library preparation kit). It is then sequenced with paired-end 2x300 reads on the MiSeq system (Illumina, USA)