Juglans ailantifolia Raw Reads Set 2
Young leaves from Juglans ailanthifolia were flash-frozen upon harvest and ground into a fine powder using liquid nitrogen. DNA was extracted using Carlson Buffer, RNase treatment, and chloroform extraction, followed by purification by Qiagen Genomic-tip columns. The final DNA was eluted in Buffer EB and stored at 4C. gDNA was extracted, quantified using Qubit, and assessed for quality via Nanodrop and Agilent Tapestation. It was sheared to ~20kb, size-selected using Circulomics SRE-XS, aiming for a 25  150 ng/L concentration. Centrifugation steps were carefully followed to recover DNA without disturbance. Buffer EB was added to the DNA pellet and incubated for re-suspension, and the DNA was then analyzed for purity and quantity using NanoDrop and Qubit methods.