GSM7885370: Flag-MDA5, Flag, rep 1; Homo sapiens; RIP-Seq
DHX9-depleted H446 cells, transfected with Flag-MDA5 or Flag-RIG-I vector, were harvested. RNA immunoprecipitation (RIP) was conducted with Flag antibody using a Magna RIP RNA-Binding Protein Immunoprecipitation kit, according to the manufacturer's instructions. RIP was also conducted with Flag antibody against H446 expressing 3xFlag-DHX9-K417R. Illumina sequencing libraries were prepared by Novogene, with the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB). Libraries were quantified using the Qubit 2.0 Fluorometer (Life Technologies).