GSM8155768: CCA_CTRL_Tum1_plate151; Homo sapiens; RNA-Seq
Tumors were dissociated using gentleMACS Dissociator and Tumor dissocation kit (Miltenyi Biotech). Cells were stained using Zombie green viability kit (BioLegend). Viable single cells from the PDX tumors treated with the CLDN1 mAb or control were FACS sorted into 384-well plates (cell capture plates) that were ordered from Single-Cell Discoveries B.V. scRNA-Seq was performed by Single-Cell Discoveries B.V. using SORT-seq protocol SORT-seq (partially robotized version of the CEL-seq2 protocol)