GSM7855405: Normal Control 1; Homo sapiens; miRNA-Seq
The total RNA was extracted using the Total RNA Purification Kit (LC Sciences, Houston, USA), according to the manufacturer's protocol. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0. Approximately 1 ug of total RNA were used to prepare small RNA library according to protocol of TruSeq Small RNA Sample Prep Kits (Illumina, San Diego, USA). And then we performed the single-end sequencing (1x50bp) on an Illumina Hiseq2500 at the LC-BIO (Hangzhou, China) following the vendor's recommended protocol.