GSM8155328: PFA 33980 | 005 | Full ROI; Macaca mulatta; OTHER
5um sections were cut from Paraformaldehyde-Fixed Paraffin-Embedded slides.  Samples were incubated with either DNA-oligo barcoded RNA-ISH probes or antibodies which were conjugated with a UV-photocleavable linker following standard ISH protocols, along with fluorescently labeled antibodies for visualization of morphological structures. Regions of interest within the tissue were illuminated with UV light and oligo barcodes were physically aspirated from the tissue and collected into microtiter plates by the GeoMx® Digital Spatial Profiler (DSP) platform. For more information about DSP protocols please see Merritt et al. Nature Biotech 2020 (doi: 10.1038/s41598-020-63539-x) Spatial transcriptome sequencing libraries were prepared with Nanostring reagents according to manufacturer's specifications