RNA-seq investigating the influence of astrocytes on developing cortical neurons
RNA-seq investigating the influence of astrocytes on developing cortical neurons
Primary cell cultures of cortical neurons and cortical astrocytes were generated from tissue collected from embryonic day 17.5 CD1 mice and embryonic day 20.5 Sprague-Dawley rats. RNA extraction was carried out using the High Pure RNA Tissue kit (Roche) as per manufacturer's instructions. Barcoded RNA-seq libraries were prepared by Edinburgh Genomics using the Illumina TruSeq stranded mRNA-seq kit, according to the manufacturer's instructions.