sRNA-Seq of Arabidopsis thaliana: MIR168inv_171stem-loop over-expressor
To create cDNA libraries for sequencing, high quality RNA samples were purified with the phenol-chloroform method from 0.3 g of bulked seedlings of respective homozygote lines. 20 g of the samples were loaded onto separate poly-acrylamide gels, the small RNA fraction was isolated and libraries were prepared only from this fraction using the Truseq Small RNA Library Preparation Kit (Illumina, San Diego, CA, USA) and the modified protocol described earlier (Czotter et al., 2018).