GSM6807414: plasma, PCH patient 3; Homo sapiens; ncRNA-Seq
The total RNA was extracted with Trizol following the manufacturer's instructions. 1-2 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared following manufacturer's protocols.Sequencing libraries are size-selected for the RNA biotypes to be sequenced using an automated gel cutter.  The libraries are qualified and absolutely quantified using Agilent BioAnalyzer 2100.