16S A.cervicornis
Bacterial composition was evaluated from amplicon libraries constructed for all samples by amplifying the V3-V4 16S region. Primers 341F and 805R with Nextera XT-compatible overhangs (TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG and GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC, respectively, with the locus-specific portion of the oligonucleotide underlined) were used to amplify bacterial 16S through PCR.