RNA-seq of human gut metagenome
The variable V3-V4 region of the bacterial 16S rRNA gene sequences was amplified by PCR with the primer pair MiSeq341F and MiSeq805R. The 5'-ends were Illumina adapter sequences while the 3'-ends of the primers amplified the V3-V4 region of the 16S rRNA gene. The amplicons were paired-end sequenced (3x200bp) using Illumina MiSeq. Quality control on the pooled library was performed using Qubit and TapeStation.