GSM7070262: rep7868_virus_aav2_capsid_biol_rep_2; Adeno-associated virus; OTHER
NaCl was added to HEK293T cells. Cells were incubated at 37C for 3 hours to lyse. The resulting material was incubated O/N at 4C. The following morning precipitates were removed and discarded. The supernatant was filtered through a 0.2 um PES filter. PEG was added to precipitate viral particles. The following day, viral genome-containing particles were purified by iodixanol gradient ultracentrifugation. Viral genomes were release by incubation of purified viral particles at 98C for 10 minutes prior to barcode amplification for sequencing. Illumina adapters were added via PCR