GSM8207447: pash-1(ram33), 20C, rep 2; Caenorhabditis elegans; ncRNA-Seq
Worms were washed from plates and rinsed with M9 3 times and flash frozed in liquid nitrogen. RNA was isolated using trizol/chloroform and isopropanol precipitation. Libraries were then prepared from total RNA according to the NEBNext® Multiplex Small RNA kit protocol. PCR products (~134-148 bp) were size selected using 10% polyacrylamide gels. small RNA-seq