GSM6415341: Ctrl1; Mus; miRNA-Seq
Total miRNA was collected from fat cells using mirVnanTM miRNA kit (AM1561, Thermo Fisher Scientific, USA) and purified using an RNeasy mini kit (Qiagen, Valencia, CA, USA). A miRNA Library was generated using the HiSeq 2500 system according to the manufacturer's instructions. Quality controls were performed and miRNA libraries were then sequenced at 10pM on the Illumina HiSeq 2500 to generate 150bp paired-end reads. miRNA-seq libraries were prepared RNeasy mini kit.