GSM7152996: BCAA-control, 2W-sham, rep2; Mus musculus; RNA-Seq
Trizol extraction of total RNA followed by polyA selection 1. mRNA enrichment, mRNA fragmentation, random priming. 2. First and second strand synthesis. 3. End repair, 5'phosphorylation, and dA-Tailing. 4. Adaptor ligation, PCR enrichment, and sequencing.