GSM6416134: Primary cortical cells, DRB-2hrs, BDNF-1hr, rep2; Mus musculus; RNA-Seq
Cells were lysed using Trizol reagent (Sigma, T9424). Total RNAs were isolated and DNase treated on columns (RNeasy micro kit, Qiagen, 74004) following the manufacturer's instructions. Library preparation was performed, starting from 200ng total RNAs, using the TruSeq Stranded mRNA Library Kit (Illumina, 20020595) and the TruSeq RNA UD Indexes (Illumina, 20022371).