COI amplicon sequencing of an insect mock community
Amplicon libraries were prepared using a two-step PCR approach, as described in Marquina et al. (2021). We amplified a 458-bp region of the COI gene using BF3-BR2 primers (Elbrecht et al., 2019) and sequenced the library pools on Illumina MiSeq using Reagent Kit v3 (2300bp reads).