GSM7823445: M_Cst7_KO_Day21pi (CellPlex Multiplex Capture); Mus musculus; RNA-Seq
Tissue was extracted from isofluorine euthanized mice after saline cardiac perfusion. Single cell suspensions were generated from tissue samples by grinding with frosted microscope slides. Immune cells were then enriched via a 2-step Percoll cushion (90% and 63%) and cells were collected at the interface of the two Percoll layers. These cells were then FACS sorted for CD45+DAPI- using CD45-APC conjugated Flow antibodies.