GSM7156192: UV165; Homo sapiens; RNA-Seq
We sampled 100-200 ul of the inflamed anterior chamber fluid using a 25- to 30-gauge needle. We pelleted cells by centrifugation (400 x g, 5 min) to remove the aqueous fluid and cryopreserved cells in fetal bovine serum containing 10% DMSO. We used the 10x Genomics 5'v2 gene expression kit according to the manufacturer's protocol.