GSM7774495: MO-32.1G.A; Rattus norvegicus; RNA-Seq
RNA was extracted from Neurospheres using TRIzol and pipet homogenization. Glycogen was added to the TRIzol sample after homogenization to aid in RNA recovery.  RNA quantification was assessed using Epoch Biotek spectrophotometer (Biotek Instruments, Winooski, Vermont) RNA was QC analyzed by Bioanalyzer (Agilent, Santa Clara, CA). To isolate polyA RNA for library preparation, NEBNext Poly(A) mRNA Magnetic Isolation Module (New England BioLabs, Ipswich, MA) was used with 1 µg good quality total RNA as input. The polyA RNA was enriched using SMARTer Apollo automated NGS library prep system (Takara Bio USA, Mountain View, CA). Next, NEBNext Ultra II Directional RNA Library Prep kit (New England BioLabs) was used for library preparation under PCR cycle number of 8