GSM8016134: RNVU1-18-/- rep2; Homo sapiens; RNA-Seq
The cell samples were lysed in TRlzol and extract total RNA for RNA-seq rRNAs were removed by Vazyme Ribo-off Globin & rRNA Depletion Kit (Human/Mouse/Rat) (Cat. No: Vazyme N408), and the VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina (Cat. No: Vazyme NR604) was used for library construction following the manufacturer's instructions with the following modifications: 1. The secondary structure of RNA was opened at 80°C for 2 minutes, but the RNAs were not fragmented. 2. The library was conducted with a broader range (200~1000bp) selection using Agencourt AMPure XP Reagent (Cat. No: Beckman A63881). 3. Gel electrophoresis was performed to obtain a library within the range of 300~400bp.