GSM7156101: HCT116_THUMPD2KO2_2; Homo sapiens; RNA-Seq
Total RNA was extracted using TRI reagent (Sigma) according to the manufacturer's instructions. DNase treatment was performed using TURBO DNase at 37°C for 15 min and RNA was re-purified using the RNA clean and concentrator kit (Zymo Research) according to the manufacturer's instructions. PolyA-enrichment was performed and libraries were constructed by TruSeq total RNA library preparation (Illumina) according to the maufacturer's instructions.