GSM8067591: A375OverN2; Homo sapiens; miRNA-Seq
cytoplasmic and nuclear fraction was prepared and RNA extracted from HEK293, A375 and SHSY5Y cells. Small RNA libraries was prepared. In terms of HEK293 cells,  AGO proteins were  also enriched by immunoprecipitation with T6B peptide. After washing the beads they were treated with Trizol and the RNA extracted. 3'adapter and 5'adapter were added generating small RNA cDNA library preparation Ligation products were reverse transcribed and amplified by PCR.