GSM6606141: E14 TKO cells, D0, biological replicate 1 (BulkRNAseq_NPC); Mus musculus; RNA-Seq
RNA extraction was performed on cell pellets using standard Trizol (Life Technologies)-chloroform protocol. RNA pellet was treated with DNase before clean up with RNeasy Mini kit (QIAGEN). RNA-seq libraries were prepared from a minimum of 200 ng of DNase-treated total RNAs with the TRuSeq Stranded mRNA protocol (Illumina)