GSM7823014: 02-mo thymus - day7 [visium]; Mus musculus; OTHER
Visium spatial gene expression slides were permeabilized at 37 °C for 12–18 min and polyadenylated. mRNA was captured by primers bound to the slides. RT, second-strand synthesis, cDNA amplification and library preparation proceeded using the Visium Spatial Gene Expression Slide & Reagent Kit (10X Genomics PN 1000184) according to the manufacturer's protocol. After evaluation by real-time PCR, cDNA amplification included 11–12 cycles. Sequencing libraries were prepared with 8 cycles of PCR. Indexed libraries were pooled equimolar and sequenced on a NovaSeq 6000 in a PE28/120 run using the NovaSeq 6000 S1 Reagent Kit (200 cycles; Illumina).