GSM6754028: XylemSclerenchymaR4_Wray_74DAP; Sorghum bicolor; RNA-Seq
Full-length cDNA synthesis, fragmentation of synthesized cDNA, and indexing were performed using SMART-Seq® v4 PLUS Kit (cat# R400753) for a RNA-Seq library construction, according to the manufacturer's protocol. Single-end read sequencing with the read length of 150 bp was performed on NextSeq 550 Sequencing System using NextSeq 550 High Output v2 kit 150 cycles (cat#20024907). As a result, cell-type transcriptomes with the total read of 23870982 on average were generated. Bulk RNA-Seq for the whole stem tissue from the same internodes was performed following the same pipeline but without LCM-based target cell type enrichment process. Sequencing for the whole stem tissue generated the average library size of 31538064 reads.