GSM6421438: starved cells, 2 hours after refeeding rep2; Tetrahymena thermophila; RNA-Seq
For RNA isolation, cell pellets were resuspended in 1 ml RNAlater and placed at 4˙C overnight. RNAlater was removed and RNA prepared using the Qiagen RNeasy kit with additional DNAse digestion on the column. 10 ug of total RNA were used to make cDNA library was prepared using Illumina RNA-seq Prep Kit (Illumina).