GSM7034773: SetxKO testis, 10dpp, rep4; Mus musculus; RNA-Seq
Testicular cell samples were resuspended in QIAzol Lysis Reagent according to the manufacturer's instructions, and total RNAs were purified from homogenized cells using Qiagen RNeasy Mini Kit. The mRNA sequencing libraries were prepared using the Illumina TruSeq methodology. mRNAs were purified from total RNA using biotin tagged poly dT oligonucleo- tides and streptavidin coated magnetic beads. The mRNAs were then fragmented and double stranded cDNA was generated by random priming.