GSM7106025: FNV_biol_rep1; Mus musculus; ATAC-seq
Cells were collected with 0.05% Trypsin, washed with DPBS, pelleted for use. 9x10^5 cells were used as starting cells. Libraries were prepared using Illumina Tagment DNA Enzyme and Buffer Small Kit (#20034197), amplified using KAPA HiFi plus dNTPs - commercial kit (#KK2102) using indexes from the TruePrep Index Kit V2 for Illumina (#TD202). Post amplification libraries were size seleced with Agencourt AMPure XP - Beckman Coulter using 0.55x/1.5x ptotocol. Library concentrations were confirmed with Qubit™ dsDNA HS Assay Kit (#Q32854) and quality were validated by Agilent 2100 Bioanalyzer.