16S rRNA gene seq human saliva 6
The Illumina amplicon library was performed following the 16S rRNA gene Metagenomic Sequencing Library Preparation Illumina protocol (Part #15044223 Rev. A).The primer used were 16S Amplicon Bakt_341F and Bakt_805R. 25 cycles of PCR amplification were performed (denaturing at 95 ?C for 30 sec, annealing at 55 ?C for 30 sec and extension at 72 ?C for 30 sec) and after cleanup an additional 8 cycles were performed to attach the dual indices and Illumina sequencing adapters. Following amplification, DNA was sequenced with an Illumina MiSeq Sequencer according to manufacturer's instructions using the 2 x 300 bp paired-ends protocol.