ATAC-seq of human cell with different genotype
Fifty thousand cells were washed in ATAC-seq resuspension buffer (Table S10) were resuspended in 50 _L of in ATAC-seq resuspension buffer containing 0.1% NP-40, 0.1% Tween-20 and 0.01% digitonin, and incubated on ice for 3 min. After lysis, nuclei were wash with cold ATAC-seq resuspension buffer containing 0.1% Tween-20 but no NP40 or digitonin, subsequently resuspended in 50 _L of transposition mixture (Table S10) and incubated at 37_C for 30 minutes in a thermomixer with 1000 RPM shaking. Fragmented DNA were then extracted with Zymo DNA Clean and Concentrator-5 kit (Zymo D4024). NGS libraries were prepared with KAPA Hyper Prep Kits (Roche KK8502) using 10 cycles of PCR amplification. Libraires were validated on a Tapestation 4200 (Agilent G2991BA), quantified by Qubit High Sensitivity dsDNA Kit (Life Technologies Q32854), multiplexed and sequenced on a NovaSeq6000 using 100-10-10-100bp reads format.