GSM7622023: EXOSC2 AID ESCs, H3K27ac, 20 hr, rep1; Mus musculus; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated and subjected to immunoprecipitation. After elution and reverse-crosslinking, the DNA was purified using DNA Clean-up Kit (CWBIO). ChIP-seq libraries were prepared using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme) following manufacturer's protocols.