GSM7916474: Day10_EB_Lmna_KO_scRNA_seq_r1; Mus musculus; RNA-Seq
Day10 EBs differentiated shControl, shControl Lmna-/-, shCth Lmna-/- mESCs were digested with Collagenase I . Cell solutions were FACS sorted for single live cells with SYTOX™ Blue Dead Cell Stain (1:2000, Thermo Fisher Scientific, S34857) and then diluted at a density of 1000 cells/µl in 0.4% BSA. 8000 cells were used for library preparation according to the Chromium Next GEM Single Cell 3' Kit v3.1 (10xGenomics, 1000269) with the Chromium Next GEM Chip G Single Cell Kit (10xGenomics, 1000127) and sequenced on a BGISEQ-500 platform.