Exome sequencing of chordoma families and sporadic cases: Sample CHR_CHR_5477_1061_A
WES was performed using SeqCAP EZ Human Exome Library v3.0 (Roche NimbleGen, Madison, WI) for exome sequence capture. The captured DNA was then subject to paired-end sequencing utilizing the Illumina HiSeq2000 sequencer for 2 X100-bp sequencing of paired-ends. Exome sequencing was performed to a sufficient depth to achieve a minimum coverage of 15 reads in at least 80% of the coding sequence from the UCSC hg19 transcripts database.