16S rRNA sequencing of fecal sample F4
V3-V4 16S rRNA amplicon gene sequencing was performed at the Genomics Core Facility of the University of Nebraska Medical Center using Illumina MiSeq utilizing the 600 cycle v3 MiSeq kit. The following primer sequences were used for the 16S library prep protocol: Forward Primer = 5'TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG; Reverse Primer = 5'GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC