GSM8010072: BMDM, PPARg WT, ChIP; Mus musculus; ChIP-Seq
BMDMs were fixed with 1% formaldehyde in culture medium at room temperature for 10 min. Subsequently, the crosslinked cells were collected, lysed and digested with MNase. The sheared chromatin was subjected to immunoprecipitation with a rabbit anti-PPARγ antibody (#81B8, Cell Signaling Technology) or rabbit IgG control overnight at 4 °C with constant rotation. The isolated complexes were collected with Protein G magnetic beads and eluted by incubating at 65 °C in a buffer with high salt concentration. ChIP-seq library preparations were constructed according to the manufacturer's protocol.