RNA-seq of 8 dpa seed from CCRI35 cultivar (repeat 3)
After total RNA was extracted, eukaryotic mRNA was enriched by Oligo(dT) beads. while prokaryotic mRNA was enriched by removing rRNA by Ribo-ZeroTM Magnetic Kit (Epicentre, Madison, WI, USA), Then the enriched mRNA was fragmented into short fragments using fragmentation buffer and reverse transcripted into cDNA with randomn