GSM6415501: sc_A25: BY4741, WT, 2-hour; Saccharomyces cerevisiae; RNA-Seq
Yeast cells were lysed with ~4µl lysis buffer, containing 0.5%Triton, 2.5µM oligo-dT, 2.5mM dNTP (Invitrogen,1959189), 8000 molecules of external RNA control consortium (ERCC) spike in, 3 x 10-2U/µl zymolyase(ZYMO, E1004-A), 1U/µl Recombinant RNase Inhibitor(TaKaRa, AI41189A), and RNA released. RNA libraries for RNA-seq were prepared using Smart-seq2 following manufacturer's protocols.