ChIP-seq of primary follicular lymphoma
Primary follicular lymphoma biopsies were processed and cryopreserved in aliquots by the University of Texas MD Anderson Cancer Center Lymphoma Tissue Bank. Frozen follicular tumor cells were thawed, and tumor B cells were sorted by gating CD20+ CD19+ CD10+ CD5- population.  Sorted tumor B cells were subsequently performed with molecular profiling. H3K27Ac loading on chromatin was profiled using previously described Mint-ChIP. Briefly, 100 thousands primary cells were processed with MNase to fragment native chromatin. Barcoded adaptors were ligated first, followed by ChIP. After ChIP, DNA samples were linearly amplified by RNA in vitro transcription and isolated for reverse transcription using random hexamer. cDNA was further amplified to the library following previously described protocol. CREBBP/EP300 loading on chromatin was profiled using modified CUT&RUN in this manuscript. All libraires were validated on a Tapestation 4200 (Agilent G2991BA), quantified by Qubit High Sensitivity dsDNA Kit (Life Technologies Q32854), multiplexed and sequenced on a NovaSeq6000 using 100-10-10-100bp reads format.