GSM6415367: KD2; Mus musculus; RNA-Seq
The aortic tissue of the mice was washed with 4°C normal saline solution containing 3% FBS (Thermo Fisher Scientific, Inc.) to remove attached blood clots. The tissue was cut into pieces as small as possible and dissociated into individual cells according to the protocol of the 10 × Genomics platform The double-ended sequencing mode of the Illumina sequencing platform was used to carry out high-throughput sequencing of the constructed libraries.