RNA-seq of 32 dpa seed from CCRI35 cultivar (repeat 3)
After total RNA was extracted, eukaryotic mRNA was enriched by Oligo(dT) beads. while prokaryotic mRNA was enriched by removing rRNA by Ribo-ZeroTM Magnetic Kit (Epicentre, Madison, WI, USA), Then the enriched mRNA was fragmented into short fragments using fragmentation buffer and reverse transcripted into cDNA with randomn