WGS of isolates from disease outbreak in cultured seabass
The library preparation workflow for the platform consisted of fragment selection, end repair and A-tailing, bubble adaptor ligation, PCR amplification, splint circularization, digestion and purification and DNB making. Raw reads were filtered if more than 25% matched the adapter sequence, if more than 50% bases had quality values lower than 20 and if there were more than 3% N in the read.