GSM8156385: Pooled plasma exosome RNA, exercise mice; Mus musculus; ncRNA-Seq
The blood was centrifuged at 2500 × g for 10 min at room temperature (RT). EVs were isolated using qEV10 columns from ~10 ml of pooled exercised and sedentary mouse plasma (from 20 mice in each group). EV-rich fractions (1-3) were concentrated using Amicon Ultra-15 Centrifugal Filters to a volume of 600 µL before RNA was extracted using the qEV RNA Extraction Kit according to the manufacturer's instructions (IZON Science, NZ). Small RNA NGS libraries were prepared using Illumina's TruSeq Small RNA Library Prep kit and were single-end sequenced at a read length of 50 nucleotides (nt).