BGISEQ-500 paired end sequencing; RNA-seq samples of ten tissues for B104 and the B73 version 5 control
RNA-seq samples of ten tissues for B104 and the B73 version 5 control
For each biosample, total RNA was isolated from cryo‐ground tissue by buffered thiocyanate salts and phenol‐chloroform extractions as previously described in 10.1371/journal.pgen.1002208. All samples were grown in the field in clay-loam soil. Sample genotypes were verified, and tissue organs collected on the dates indicated. mRNA enrichment and purification was performed by BGI using Oligo dT Selection to enrich the mRNA. Oligo dT Library construction was performed by BGI with the BGISEQ NGS platform.