GSM7068918: BC187_NaCl_Gen0_Rep3; Saccharomyces cerevisiae; OTHER
Plasmids were isolated from cell pellets using the Qiagen Spin MiniPrep Kit. Libraries were constructed using PCR where plasmid barcodes were amplified with multiplex primers containing Illumina adapters and unique 10bp index tags to allow for sample identification after sequencing, as described in Magtanong et al., 2011 and Piotrowski et al., 2015.