Whole-genome sequencing of fluconazole-resistant Candida parapsilosis isolates
Genomic DNA was isolated utilizing a Triton SDS and phenol-chloroform method previously described. {Amberg 2006} DNA concentrations were quantified using both the Qubit Fluorometer and Nanodrop spectrophotometer using the manufacturers protocols. Whole genome libraries were prepared and sequenced on the NovaSeq600 platform (150bp, paired-end reads)