RNA-Seq of Bacillus cereus: Paris Polyphylla saponin production
The second strand was synthesized by replacing dTTP with dUTP after the first strand cDNA synthesis, and joint ligation was performed. The products were purified using VAHTS DNA Clean Beads. The U-strand was digested, amplified using PCR, and the product was purified again using VAHTS DNA Clean Beads. The library quality was assessed using Agilent 2100.