GSM6605586: Chap, PTEN KO/KO, week 10; Homo sapiens; RNA-Seq
Cortical organoids were dissociated into single-cell suspension with the Papain dissociation system (Worthington Biochemical, LK003150). Cells were then loaded onto the Chromium Next GEM Chip G Single Cell Kit, (10x Genomics, 1000120), and were processed with Chromium Controller to generate single-cell gel beads in emulsion. The Chromium Next GEM Single Cell 3' Kit v3.1 (10x Genomics, 1000268) was used for all scRNA-seq library preparations.