DNBSEQ-G400 paired end sequencing; RNA Spatial Sequencing of Colorectal Liver Metastases regarding their Histopathological Growth Patterns
RNA Spatial Sequencing of Colorectal Liver Metastases regarding their Histopathological Growth Patterns
The Tissue was collected from the tumor border, capturing both tumor and peritumor healthy tissue per slide. The Visium Spatial Gene Expression for FFPE Kit (10X Genomics, PN-1000338, chemistry v1) was used to generate sequencing libraries. Prior to section placement, tissue adhesion was assured using the Visium Tissue Section Test Slides (10X Genomics, PN-1000347) and RNA-quality of FFPE blocks was assessed using RNeasy FFPE Kit (Qiagen, #73504) for RNA extraction and Agilent 2100 Bioanalyzer system with Agilent RNA 6000 Nano Kit (5067-1511) for determination of DV200. All samples reached DV200 of at least 50%.  Nucleic acid extraction was performed using the 10xGenomics Visium Spatial Gene Expression Reagent Kits following the given protocol (CG000239). Nucleic acid library construction was performed using the 10xGenomics Visium Spatial Gene Expression Reagent Kits following the given protocol (CG000239).