Illumina NovaSeq 6000 paired end sequencing; RNA-seq from human peripheral blood isolated and CRISPR/Cas9-mediated BCL11B depleted alpha-beta CD8+ T cells and corresponding not transfected (nt) and mock (mock gRNA transfected) controls.
RNA-seq from human peripheral blood isolated and CRISPR/Cas9-mediated BCL11B depleted alpha-beta CD8+ T cells and corresponding not transfected (nt) and mock (mock gRNA transfected) controls.
Cells were counted via flow cytometry and harvested using wide bore tips to reduce pipetting-induced stress. Cell numbers were adjusted to 10000 cells per sample. According to the manufacturer's instructions: Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry) https://www.10xgenomics.com/support/single-cell-gene-expression/documentation/steps/library-prep/chromium-single-cell-3-reagent-kits-user-guide-v-3-1-chemistry According to the manufacturer's instructions: Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry) https://www.10xgenomics.com/support/single-cell-gene-expression/documentation/steps/library-prep/chromium-single-cell-3-reagent-kits-user-guide-v-3-1-chemistry