GSM8014569: Control044; Homo sapiens; MeDIP-Seq
Total genomic DNA was extracted from 0.5 ml of whole blood using Qiagen DNeasy Blood & Tissue Kits. The total genomic DNA was ultrasonic fragmented to 150-300 bp using Covaris M220 machine, following by end-repair and A-tailing using Kapa HyperPrep Kit (Kapa Biosystems). The A-tailed DNA were then ligated with the NEBNext adaptor, purified with AMPure XP beads (Beckman Coulter) and then digested with USER enzyme (New England BioLabs) to release the dU loop structure of the adapter. The products were further purified using Qiagen MinElute PCR Purification Kit. Subsequently, MeDIP immunoprecipitation using MagMeDIP kit (Diagenode) was performed following previously described protocols. An average of 100ng of fragmented DNA were used for the MeDIP immunoprecipitation