GSM8328359: Rnaseq, Sp140 KO, untreated, rep 1; Mus musculus; RNA-Seq
Total RNA was extracted from cells either untreated or treated for 8 hours with 100 ug/mL DMXAA with the Omega Biotek Total RNA II kit. Genomic DNA was removed from RNA by treatment with TURBO Dnase (thermofisher). Libraries for RNA-seq were generated by Azenta. Single-index libraries were generated using rRNA depletion (Qiagen, QIAseq FastSelect – HMR rRNA Removal kit) and the NEBNext RNA Ultra kit (NEB). For ATAC-seq, libraries were generated as described (Buenrostro 2015), with additional purification with Ampure XP beads to remove contaminating adaptor dimers.