Mitochondrion-Seq of Onychoteuthis compacta musculus: carcass
1. The genomic DNA was processed into 15-20k fragments by G-tubes method;2. The ends shall be leveled, and the two ends shall be connected to the circular single chain respectively: the two ends of the single chain shall be connected to the double chain positive and negative chains respectively to obtain a structure similar to the dumbbell ("horse ring"), called SMRT Bell3. Anneal the single link of the library and bind it to the polymerase at the bottom of the fixed ZMW (zero mode waveguide holes).4. Computer sequencing can be performed after combination