RNA-seq of colon in Mice
10 mg of colonic tissue was taken from each group, 1ml of Trizol was added, and the tissue was well ground and crushed at -30 in a freezing grinder. Total RNA was extracted from the colonic tissue, and the concentration and purity of RNA were detected by an enzyme marker. Reverse transcription to cDNA was used. qRT-PCR experiments were performed using SYBR green reagent. Reaction conditions: pre-denaturation at 95 for 30s, denaturation at 95 for 10s, annealing at 55 for 30s, and extension at 72 for 15s, with 45 cycles.