GSM7067918: 5016_LI_EDTA; Mus musculus; ATAC-seq
Intestine tissues were first incubated in 5 mM EDTA in HBSS containing 1 mM DTT for 20 min at 37°C with shaking, and then incubated in a second wash of 5 mM EDTA in HBSS without DTT for 20 min at 37°C with agitation. Suspended cells from the EDTA washes were pooled as enriched “intestine epithelial cells”. ATAC-seq libraries were generated as described by J. D. Buenrostro, B. Wu, H. Y. Chang, W. J. Greenleaf, ATAC-seq: A Method for Assaying Chromatin Accessibility Genome-Wide. Curr Protoc Mol Biol 109, 21 29 21-21 29 29 (2015).