GSM8155309: CD45+ brain leukocytes GEX 33994; Macaca mulatta; RNA-Seq
Nuclei were isolated from brain derived live CD45+ and CD45- cells using the Chromium Nuclei Isolation Kit (10X Genomics) using manufacture instructions. Following isolation, nuclei were stored on ice and used immediately for subsequent library preparation. Barcoded 3' single cell gene expression library and ATAC library were prepared from single nuclei suspensions using the Chromium Single Cell Multiome kit (10X Genomics) following the manufacturer's instruction. The libraries were quantified by fluorometry on a Qubit instrument (LifeTechnologies) and by qPCR with a Kapa Library Quant kit (Kapa Biosystems-Roche) prior to sequencing. The libraries were sequenced on a NovaSeq 6000 sequencer (Illumina) with paired-end 150 bp reads with approximately 35,000 reads pairs per nuclei for gene expression and 25,000 read pairs per nuclei for ATAC libraries. 10X single nuclei multiome (snRNASeq and snATACSeq)