GSM6481270: HC biol rep3; Mus musculus; RNA-Seq
The offspring mice were raised to adulthood (8W) after birth. After being killed by cervical dislocation, the liver and kidney tissues were retrieved and thoroughly washed with normal saline to remove the blood. Then, the tissue block was cut 0.5 cm×0.5 cm in size with sterile RNase-free surgical scissors, ground into a powder after adding liquid nitrogen, and collected in a sterile RNase-free EP tube. After TRIzol was added and fully lysed, the total RNA in the tissues was obtained for sequencing according to the conventional RNA extraction steps. The library was built according to standard Illumina protocols, which mainly include the steps of rRNA removal, mRNA isolation and fragmentation, cDNA double-strand synthesis and purification, cDNA terminal repair, 3' end plus A, adaptor ligation, screening cDNA fragments of around 200 bp, PCR library enrichment and purification, etc.