GSM8289913: HUH7_Input; Homo sapiens; RNA-Seq
RIP assay was performed using the BersinBioTM RNA Immunoprecipitation (RIP) Kit (BersinBio, Guangzhou). Total RNA from the HUH7 cells  was extracted using Trizol reagent (Thermo Scientific, MA, USA) The enriched RNA was fragmented using Fragmentation Buffer into short fragments. The fragmented mRNA was then used as a template to synthesize single-stranded cDNA using a random hexamer primer. The double-stranded cDNA was synthesized by adding buffer, dNTPs, and DNA polymerase I. The purified double-stranded cDNA was subjected to end repair, followed by addition of an A-tailing and sequencing adapter treatment. Target-sized fragments were recovered using magnetic beads and amplified through PCR. The constructed libraries were quality-checked using agarose gel electrophoresis. The library concentration was quantified using Qubit 2.0 to ensure suitability for sequencing. Upon passing quality control, different libraries were sequenced on the Illumina sequencer according to the desired effective concentration and target data volume.