GSM8365536: Mlcl_Rxr_1; Mus musculus; OTHER
Cells were isolated by 0.25% trypsin/EDTA, pelleted and washed with PBS. Cells were pelleted and were cross-linked. Cut-&-Run libraries were prepared on these cross-linked nuclei, according to Skene, P. J. & Henikoff, S. An efficient targeted nuclease strategy for high-resolution mapping of DNA binding sites. Elife 6 (2017). CNR sequencing libraries were generated using NEBNext Ultra II DNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina. PCR-amplified libraries were purified using 1X ratio of SPRI beads (Beckman) and eluted in 15 μl EB buffer (Qiagen). All CNR libraries were sequenced on Illumina NextSeq using 40 bp paired-end reads.