GSM8156113: P1SV, replicate 1, scRNAseq; Mus musculus; RNA-Seq
Inner ears from a total of four P1 mice and four P30 mice were collected. The SV was micro-dissected from the spiral ligament and lysed in either 0.5 mg/ml trypsin at 37°C for 7 min for P1 tissue, or 400-600 units/mL accutase at 37°C for 25 min for P30 tissue. Media was gently replaced with 5% FBS in DMEM to stop lysis, and the tissue was triturated and then filtered using a 20 µm filter (pluriSelect Life Science, El Cajon, CA, United States), and the cells were kept on ice for 35 min. The cell pellet was then suspended in 50 µL of the filtered media and cell counts were performed using a Luna automated cell counter (Logos Biosystems, Annandale, VA, United States). A cell density of 1 x 106 cells/ml was used to load onto the 10X genomics chip. Library preparation was performed according to the instructions in the 10x Genomics Chromium Single Cell 3' Chip Kit V2.