GSM8253647: Cerebellum, Cxcr4+/1013; Mus musculus; RNA-Seq
Freshly microdissected lobule III-IV regiond form Cxcr4+/- and control mice cerebella at P1 in ice cold HBSS solution (Mg2+/Ca2+ free supplemented with 10mM HEPES, pH 7.2). The tissues were dissociated to single cell suspension using Papain Dissociation System kit (Worthington-Biochemical, #LK003150). Library was performed according to the manufacture's instruction of Chromium Next GEM Single Cell 3' kit  v3.1 (10X Genomics, #1000269). 3' gene expression scRNA-seq