GSM7106954: Wildtype replicate 1; Danio rerio; Bisulfite-Seq
DNA was isolated using ZymoResearch DNA isolation kit. RRBS library preparation and sequencing were conducted by NXT-Dx, a Diagenode company (Ghent, Belgium). Briefly, libraries were prepared from 500 ng of genomic DNA digested with 30 units of MspI (New England Biolabs, Massachusetts), followed by end repair and A-tailing of DNA fragments. Fragments were ligated with methylated Illumina adapters. Adaptor-ligated fragments were size selected and then bisulfite converted using a commercial kit. Ligated fragments were amplified and the resulting products were purified and 50-bp paired-end sequencing was performed on an Illumina HiSeq2500 platform. Sequence reads from RRBS libraries were identified using standard Illumina base-calling software (Cassava v.1.8.2).