GSM7107785: 60min_V5Rap ChIP rep2; Saccharomyces cerevisiae; Saccharomyces paradoxus; ChIP-Seq
Cells were lysed by mechanical disruption in a bead beater. Chromatin was fragmented by sonication. All immunoprecipitations were performed using 50uL DynaBeads Protein G (ThermoFisher Scientific 10003D) magnetic beads coupled with 5uL monoclonal mouse-anti-V5 antibody (ThermoFisher Scientific R96025)  DNA was purified using Qiagen spin column kit NEB Ultra II library prep kit