GSM8290349: tardbp, 2TG, rep1; Danio rerio; RNA-Seq
Total RNA was then extracted from 4-dpf embryos using TRIzol (15596026, Invitrogen, USA) or RNAiso Plus (91085, Takara Bio, Japan) following the manufacturer's protocols. At least 1 μg total RNA of each sample was used for the construction of sequencing library. Construction of RNA libraries was done by the sequencing company (Anoroad) following standard procedures.