tier27_row4_tree34_gDNA
DNA of young, unexpanded leaves was extracted from 14 individuals (7 early-blooming, 5 late-blooming, and the two parents) with a DNeasy Mini Plant Kit (Qiagen, Hilden, Germany) and quantified with a Quant-iTTM PicoGreenTM dsDNA assay kit (Thermo Fisher Scientific, Waltham, MA) or using the Qubit DNA Broad Range kit and Qubit 4 Fluorometer (Thermo Fisher Scientific Inc, Waltham, MA). Quality was assessed with an Agilent 4200 TapeStation HS DNA1000 and Kapa Biosystems Illumina Library Quantification qPCR assays. Illumina TruSeq Nano DNA 150 bp paired-end (PE) libraries were prepared at MSUs Research Technology Support Facility (RTSF) and sequenced at 20X (progeny) or 60X (parents) using a HiSeq4000 instrument. At least 22.6 million paired end reads were produced for each sample and checked for quality using FASTQC