Illumina HiSeq 2000 paired end sequencing; RNA-seq experiment to evaluate the role of methylation of the spliceosome component LSM4 in the regulation of alternative splicing
RNA-seq experiment to evaluate the role of methylation of the spliceosome component LSM4 in the regulation of alternative splicing
Three biological replicates were collected. Whole seedlings were harvested. Wild-type, lsm4-1, LSM4R and LSM4RxK in lsm4-1 background seedlings were grown on Murashige and Skoog (MS) medium containing 0.8% (w/v) agar for 12 days under continuous light at 22°C. Whole seedlings were harvested and total RNA was extracted with RNeasy Plant Mini Kit (QIAGEN) following the manufacturer's protocols. To estimate RNA concentration NanoDrop 2000c (Thermo Scientific) was used.  Libraries were prepared by genome center at the Max Planck-Genome-Centre Cologne (MP-GC).