RNA-Seq of Xenopus laevis: hindlimb blastema of froglet
Library preparation was performed using TruSeq Stranded mRNA Library Prep Kit (Illumina) or Illumina Stranded mRNA Prep Ligation Kit (Illumina) with IDT for Illumina RNA UD Indexes (Illumina), and KAPA Real-Time Library Amplification Kit (KAPA Biosystems) for PCR cycle determination. The amount of total RNA used for library preparation was 500 ng. The number of PCR cycles for library amplification were 8 cycles.