snATAC of human pancreas: nPOD 6282
Roughly 40 mg of ground tissue was resuspended in 1 ml of nuclei permeabilization buffer. Nuclei suspension was filtered with a 30-m filter. Nuclei were counted using a hemocytometer, and 15,360 nuclei were used for tagmentation. snATAC-seq libraries were generated using the Chromium Single Cell ATAC Library & Gel Bead Kit v1.1 (10x Genomics, 1000175), Chromium Chip H Single Cell ATAC Kit (10x Genomics, 1000161) and indexes (Single Index Kit N Set A, 1000212) following manufacturer instructions. Final libraries were quantified using a Qubit fluorometer (Life Technologies), and the nucleosomal pattern was verified using a TapeStation (High Sensitivity D1000, Agilent). NovaSeq 6000 sequencers (Illumina) with the following read lengths (Read1 + Index1 + Index2 + Read2): 50 + 8 + 16 + 50.