GSM7611143: F_HF_CKO-1; Mus musculus; RNA-Seq
RNA was extracted from placenta tissue from MyD88-CKO dams fed either control or HFD. Biologically distinct pools were created in a genotype and sex-stratified manner (n = 3 per group). Total RNA was isolation using the TRI reagents and RNeasy Mini Kit per manufacturer's instructions including on-column DNase digestion. Total RNA was quantified using a Qubit RNA BR Assay Kit and RNA integrity was assessed on an RNA ScreenTape. mRNA-seq libraries (n = 3 per group) were prepared using a Nebnext Ultra reagents following mRNA isolation Dynabeads Oligo-Dt reagents. Manual bead cleanup was performed using AMPure XP Beads (Beckman Coulter, A63881) after the libraries were completed to remove primer-dimers. Qubit dsDNA BR Assay kit was used to determine the concentration of the completed library and a Fragment Analyzer Standard Sensitivity NGS Fragment Kit Single-end, opposing starnd libraries