GSM6657669: D melanogaster, M luteus injected, 3 hours, biol rep 3; Drosophila melanogaster; RNA-Seq
Trizol (Life Technologies) was used according to the manufacturer's instructions. Libraries were prepared using Illumina TruSeq Stranded mRNA v2, with the following changes from the manufacturer's protocol: starting samples were 250ng total RNA; fragmentation was performed for 10 minutes at 94°; no spike-ins were used; and 14 cycles of PCR were used to enrich libraries.