RNA-Seq of Bactericera cockerelli: 5th instar nymphs bacteriome
Strand-specific and barcode indexed RNA-seq libraries were generated from 300ng total RNA for each sample. Poly-A enrichment and library prep was done using the KAPA mRNA Stranded library preparation kit (KK8421, Kapa Biosystems, Cape Town, South Africa), following the instructions of the manufacturer. Libraries were amplified with 12 cycles of PCR. The fragment size distribution of the libraries was verified via micro-capillary gel electrophoresis on a Bioanalyzer 2100 (Agilent, Santa Clara, CA).  The libraries were quantified by fluorometry on a Qubit fluorometer (LifeTechnologies, Carlsbad, CA) and pooled in equimolar ratios. The pool was quantified by qPCR with a Kapa Library Quant kit (Kapa Biosystems) and sequenced on 1 lane of the Illumina NovaSeq S4 platform (Illumina, San Diego, CA) with paired-end 150bp reads.