GSM8155728: Ctrl#2-scRNA; Mus musculus; RNA-Seq
After harvesting bone marrow from the femur and tibia of both legs, the bone marrow was filtered through a 70μm filter. The GMPs were sorted from the bone marrow (CD34+ CD16/32+) by flow cytometry. Single cell RNA-seq libraries were prepared with Chromium Single Cell 5' Library kit (10x genomics). Briefly, for scRNA-Seq, sorted cells were encapsulated in droplets with reverse transcriptase, template switching oligos and beads. Full length cDNAs were synthesized by template switching in droplets. After breakage of droplet and cDNA purification, full length cDNAs were amplified.