GSM8206971: OT-I T cell Opa1 KO; Mus musculus; RNA-Seq
A physiologically relevant number (500) of congenically marked (CD45.1) OT1 T cells were adoptively transferred into a naïve B6 recipient (CD45.2). Recipient mice were infected intravenously (i.v.) with LM.Ova.ActA as described previously. On day 5, T cells were isolated and pooled from 4 mice prior to cell sorting of CD45.1+ CD8+ CD19- cells. Naïve cells were isolated using Biolegend CD8 isolation kit. Naïve and d5 cells for each genotype were pooled at a 1:1 ratio and roughly 10,000 cells were loaded onto Single Cell A chips (10X Genomics) and partitioned into Gel Bead In-Emulsions in a Chromium Controller (10X Genomics). Single-cell RNA libraries were prepared according to the 10X Genomics Chromium Single Cell 5' Reagent Kits v3.1 User Guide and sequenced on a NovaSeq 6000 (Illumina)  by the CU Anchutz Genomics Core.