GSM8365687: CASP, H2O treatment, TP 120m, Biorep3; Arabidopsis thaliana; RNA-Seq
Total RNA was extracted with a TRIzol-adapted ReliaPrep RNA extraction kit (Promega). Libraries were prepared by Lausanne Genome Technology Facility following the same protocol of Fujita et al 2020. RNA quality control was performed on a Fragment Analyzer (Advanced Analytical Technologies). 1000 ng of total RNA was used to prepare RNA-seq libraries with the Illumina TruSeq Stranded mRNA reagents (Illumina) on a Sciclone liquid handling robot (PerkinElmer) using a PerkinElmer-developed automated script.