Illumina sequencing of an Accel-NGS-amplified DNA sample of Campydora demonstrans
Live individuals of Campydora demonstrans were extracted with Baermann funnel from soil samples collected near Trebejov, Slovakia (September 1997), fixed with hot TAF (4% formaldehyde-containing fixative; Courtney et al. 1955), stored in glycerin and re-transferred in TAF after two years (material identified and kindly provided by Prof. Dieter Sturhan). Total DNA was extracted from specimens pre-washed in deionized water for 1 h, using standard worm lysis solution and a protocol (Williams et al. 1992; 10 min 70C, 1 h 65C, 10 min 95C), and stored at 20C for ~20 years until NGS library preparation. The DNA library was prepared using Accel-NGS chemistry and sequenced on an Illumina HiSeq 4000 platform following the manufacturers protocol.