Targeted amplicon sequencing of chordoma cases (paired tumor and normal): Sample CHR_5477_1140_Tumor_rawlib
Targeted panel sequencing (gene list below) with a custom-designed AmpliSeq panel using the Ion AmpliSeq Designer was conducted on paired tumor and germline DNA on the Ion Torrent S5 Sequencer, with an average coverage of 1,158x. We sequenced the entire exonic regions for these genes and deep sequence coverage was generated at each locus of interest for each sample. ARID1A,ATM,ATR,BCCIP,BRCA1,BRCA2,CDKN2A,EGFR,ERCC4,HMG20B,HNRNPC,IFNG,LYST,MAP3K4,MCM10,MET,MGA,MORF4L1,MORF4L2,PALB2,PRMB1,PIK3CA,PTEN,RAD51,RAD51B,RAD51C,RB1,SETD2,SHFM1,SMARCB1,TBXT,TERT,TP53,TSC1,TSC2,USP9X,VHL,XRCC2