Illumina amplicon next generation sequencing of Dirofilaria immitis: sample 2216-4-L15709A
First stage PCRs for Illumina Next Generation Sequencing (NGS) were performed in-house according to the 16S Metagenomic Sequencing Library Preparation instructions for the Illumina MiSeq system (Illumina, Australia). PCR products (~30 L) were submitted to the Ramaciotti Centre for Genomics, The University of New South Wales, Australia for indexing (384), library preparation and NGS using Illumina MiSeq v2 250PE sequencing run.