GSM7068334: SLAM-seq_256Cell, wildtype, replicate1; Danio rerio; OTHER
The mRNA was prepared from whole embryo pools using the RNeasy Mini Kit 493 (74104; Qiagen). The QuantSeq libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit for Illumina (Lexogen). RNA sequencing was conducted using custom primers on an Illumina Nextseq 500 with High Output chemistry and 75bp single-ended reads.