GSM7108086: U2OS-KRAB-IDR3-TCF3, H3K9me3, ABCAM, rep2; Homo sapiens; ChIP-Seq
Cells were washed in FBS-free media then cross-linked with 1% formaldehyde for 10 min at room temperature, Cross-linked cells were washed with ice-cold PBS, For each sample, 20 million fixed cells were lysed to prepare nuclear extracts. For ChIP-Seq, 5ng of purified ChIP DNA were used to generate the sequencing library using a NEB kit and sequenced with the Illumina HiSeq X Ten.