GSM8016126: Gland Tissue, DKO, Rep 1; Mus musculus; ssRNA-seq
SMGs from 5-week-old WT and Stim1/2Foxp3 male mice were harvested and kept in chilled tubes with prefilled beads in 1 mL TRIzolTM reagent. Organs were processed via tissue homogenizer for 30s to completely disassociate the tissue.  RNA was extracted with the RNeasy Mini Kit followed by cDNA synthesis using the iScript cDNA synthesis kit. RNA was then depleted of ribosomal RNA RNA libraries were prepared using automated stranded RNA-seq library prep polyA selection system. The libraries were purified with AMPure bead.