the raw data of Ldec. genome
Genomic DNA was extracted from one female pupa using the QIAamp DNA Mini Kit (QIAGEN). The integrity and purity of DNA was verified with agarose gel electrophoresis (AEG) and Nanodrop 2000. Eight micrograms of genomic DNA were sheared using g-Tubes (Covaris), and concentrated with AMPure PB magnetic beads. Each SMRT bell library was constructed using the Pacific Biosciences SMRT bell template prep kit 1.0. The constructed library was size-selected using the Sage ELF system for molecules 8-12 Kb, followed by primer annealing and the binding of SMRT bell templates to polymerases with the DNA Polymerase Binding Kit. Sequencing was carried out on the Pacific Bioscience Sequel II platform for 30 h.