GSM7948808: RPE1_sorted, mRNA-derived cDNA; Homo sapiens; RNA-Seq
On the day of harvest, cells were trypsinised into suspension, labelled with the indicated hashtag antibodies and then pooled for single-cell encapsulation with the 10x Genomics platform. Libraries were prepared according to the manufacter's instructions (single cell 3' v3 protocol, 10x Genomics), the multiplexed cell suspension was loaded onto 2 lanes n the 10x Chromium Genomics platform for single cell encapsulation and library preparation according to the manufacturer's instructions (10x Genomics Chromium Single Cell 3' GEM v3 workflow). Standard 10x Genomics parameters were used for sequencing: read1: 28bp, index1: 8bp, index2: 0bp, read2: 91bp. The libraries were sequenced on 1 lane of an S1 flowcell on an Illumina NovaSeq 6000.