GSM7884523: pTOsk-rhi-KD.3 [total RNA]; Drosophila melanogaster; ssRNA-seq
Total RNA was extracted from 10 to 20 ovaries from 3-6 day-old flies using TRizol (Thermo Fisher Scientific). Ribosomal RNA was depleted using RiboPOOL (siTOOLs, Biotech) following the manufacturer provided protocol. RNA-seq libraries were produced using NEBNext Ultra Directional Library Prep Kit for Illumina, following the manufacturer's instructions for rRNA depleted RNA.