GSM6807331: CD3D Edited 0877 FB; Homo sapiens; OTHER
ATOs were harvested at week 8 (as above) and subjected to MACs Dead Cell Removal Kit (Miltenyi, Cat. 130–090-101), and ~5 x 105 cells were stained with TotalSeq-C Human Universal Cocktail, V1.0 (Biolegend, Cat. 399905) per the manufacturer's protocol. Labeled cells were submitted to the UCLA Technology Center for Bioinformatics and Genomics for unique molecular identifier (UMI) tagging and generation of gene expression (GEX), human TCR repertoire (VDJ), and Feature Barcoding libraries using the 10X Chromium Next GEM Single Cell 5' Kit v2 (10X Genomics, Pleasanton, CA). Fully constructed libraries for all samples were run in one S4 flowcell on the Illumina Novaseq platform. 5' Gene Expression (GEX), VDJ, and Feature Barcoding libraries were constructed according to the manufacturer's instructions (Chromium Next GEM Single Cell 5' Reagent Kits v2, 10X Genomics).