Euplectellid hexactinellid (tulip sponge) MluCI Hi-C library
We generated one DpnII-based Hi-C library abd one MluCI-based Hi-C library for the hexactinellid sponge using the Hi-C preparation protocol described in the article: Adams, Matthew, et al. "One flyone genome: chromosome-scale genome assembly of a single outbred Drosophila melanogaster." Nucleic acids research 48.13 (2020): e75-e75. For this we used sponge tissue that had been flash-frozen in liquid nitrogen. We did not pre-grind the flash-frozen tissue in liquid nitrogen before applying paraformaldehyde, but we ground the tissue with a blue pestle after placing it into the paraformaldehyde solution. Instead of PBS in the paraformaldehyde chromatin fixation solution, we used artificial seawater. These libraries were sequenced to 235x genome coverage.