GSM8156195: Native HSPC (Rep 2); Mus musculus; RNA-Seq
Bone marrow from the bilateral femur and tibia was collected; red blood cells were lysed and mature hematopoietic cells were depleted using magnetic bead-based lineage antibody cocktail.  Pre-selected cells were then flow sorted to collect Lin-/Sca1+ cKithi (LSK) HSPCs. For each replicate, 30,000 cells were collected from each experimental condition, barcoded with TotalSeqB antibodies, and multiplexed for sequencing. Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1