GSM7067983: GS359_T2_ctrl_tmz, scRNAseq; Homo sapiens; RNA-Seq
Cells were detached from culture plates with Accutase, resuspended in HBSS, and individual cells were FACS sorted into 384-well plates The libraries were constructed according to the SORTseq protocol. Briefly, single cells sorted into 384-well plates were submitted for scRNAsequencing at Single Cell Discoveries, Utrecht. Libraries were constructed based on a semi-robotized implementation of the CELseq2 library protocol, and submitted for paired-end sequencing