GSM7156096: ES2_Control+LY500307_1; Homo sapiens; RNA-Seq
ES2-control and ES2-KDM1A knockout cells were treated with vehicle or LY500307 (5 µM) for 48 hours. RNA was isolated using RNeasy mini kit and subjected to RNA-seq on illumina HiSeq 3000 system using Illumina TruSeq stranded mRNA-seq library preparation kit Total RNA samples about 1-2 micro grams were used for sequencing library preparation according to Illumina TruSeq Total RNA Sample Preparation Guide (Illumina Cat. #: RS-122-2201). Each library was bar-coded and then pooled for cluster generation and sequencing run with 50bp single-end (SE) sequencing protocol.