GSM6419828: 0h; Mus musculus; OTHER
Cells were harvested, counted and centrifuged for 3 min at 600 × g at room temperature.Aliquots of cells (60-500,000 cells), were washed twice in Wash Buffer by gentle pipetting.Cell nucleus were prepared and resuspended.Primary antibody incubation was pe To increase the number of Protein A binding sites for each bound antibody, an appropriate secondary antibody (such as Guinea Pig anti-Rabbit IgG antibody for a rabbit primary antibody) was diluted in Dig-Wash buffer and cells were incubated at RT for 30 m CUT&Tag