Nanopore sequencing of Thuja sutchuenensis: young needles
Purified DNA was prepared for longread sequencing following the protocol in the SQKLSK108 genomic sequencing kit (ONT, Oxford, UK). The purified library was loaded into a R9.4 SpotOn Flow Cell (ONT), and Oxford Nanopore GridION 5 sequencing was carried out for 48 h at GrandOmics (Wuhan, China).