GSM6807225: OI_M_Tibia_2174; Mus musculus; RNA-Seq
After mincing with scissors, the bones were homogenized in 5 mL of TRIzol™ (Thermo Fisher) for 15 s with a tissue mincer equipped with a 5-mm stainless steel generator probe. The total RNA was extracted using the phenol-chloroform method. RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols. mRNA enrichment was performed using the NEBNext Poly(A) Magnetic Isolation Module (New England Biolabs, Ipswich, MA, USA). cDNA synthesis was achieved with the NEBNext Modules (New England Biolabs). The remaining steps of the library preparation were done  using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs).