GSM8031472: H3_lhp1dekT_rep1; Arabidopsis thaliana; ChIP-Seq
Frozen seedlings were ground into fine powder. Suspension in nuclei isolation buffer and then crosslinked with 1% formaldehyde. After fiitering through a 40 µm nylon cell strainer, isolated nuclei were centrifuged and resuspended in nuclear isolation buffer. The nuclear pellets were lysed using a buffer containing detergen and sonicated using a focused-ultrasonicator. Following immunoprecipitation processes, DNA was decrosslinked from the beads by incubating overnight at 65 °C in elution buffer. Genomic DNA were recovered and purified after Proteinase K and RNase treatment. The  libraryies were made using the ThruPLEX DNA-seq Kit (Takara) with manufacturer's protocol