RNA-Seq of marine sediment: Central Reef island
Metagenomic DNA was extracted from 0.3  g of the sediment sample using the DNeasy PowerSoil kit (Qiagen, Germany). The libraries of V3-V4 region of 16S rRNA gene were prepared using NEB Next Ultra DNA Library Prep Kit (Illumina). The amplicons were sequenced into 250bp paired end reads by the Illumina MiSeqplatform.