GSM6753287: male_PiC-MeA_neurons_rep2; Mus musculus; RNA-Seq
EGFP-labeled neurons were collected into a micropipette (tip diameter 60-100um) filled with RNAse-free lysis buffer（0.2% Triton X-100 and 1µl RNase inhibitor）and then promptly transferred to an RNAse-free centrifuge tube containing 2ul of RNAse-free lysis buffer, with later total mRNA extraction. 4-5 cells were collected in one tube as a sample. RNA libraries for RNA-seq were prepared using SMART-Seq2 Library Prep Kit following manufacturer's protocols.