BT22
After obtaining a pure colony of strain BT22, we conducted a subculture in 2 mL of BHI broth medium, followed by incubation at 37C for 18 hours. Subsequently, we extracted the total genomic DNA using the DNAMITE DNA bacterial kit from Microzone Ltd (United Kingdom). Once the genomic DNA was extracted, we performed purification to obtain a purified DNA sample. The purified DNA was then eluted in 50 l of AE buffer, as part of the DNAMITE DNA bacterial kit. Before proceeding further, we verified the concentration of the purified DNA using Qubit and Nanodrop measurement devices. For the subsequent analysis, we sent the purified DNA sample to the service provider Microsynth AG, Schtzenstrasse 15, 9436 Balgach, Switzerland. Their expertise in genomic sequencing was utilized to perform the sequencing on the Illumina MiSeq platform.