RNA-Seq of rattus norvegicus: adult male heart
Heart tissue from acute IHD rats (oral administration of CM/NM/Mito@Cap group: n=4) was collected. Total RNA was extracted. The RNA purity was evaluated using a NanoDrop 2000/c spectrophotometer (Thermo Scientific), and libraries were constructed using a VAHTS Universal V6 RNA-seq Library Prep Kit.  The libraries were sequenced on a Lumina Novaseq 6000 platform, and 150-bp paired-end reads were generated. HISAT was used to map the clean reads to the reference genome.