genome sequencing Helicobacter pylori
The DNA was isolated by using the Wizard DNA extraction kit (Promega Corporation, USA) and size selected with optimized solid phase reversible immobilization (SPRI) beads. The DNA concentration and quality were determined on a Qubit 4 Fluorometer and Nanodrop ND-1000 (Thermo Fisher Scientific). The long reads were generated with MinION sequencing (Oxford Nanopore Technologies, UK). The sequencing libraries were prepared using the ligation sequencing kit SQK-LSK109, native barcoding expansion kit EXP-NBD104 and run in a FLO-MIN106 flow cell. Reads were basecalled using Guppy v3.6.1. using default parameters (guppy_basecaller).