DNA-Seq of Dku:adult female
The galls of Dryocosmus kuriphilus were collected from chestnut trees a local paddy field in Changsha, Hunan province, China, during May to June 2019. The collected gall was placed in a breeding cage (length  width  height =30 cm  20 cm  30 cm) at 25 , and the emerging adult worms were frozen in liquid nitrogen at -80  for subsequent sequencing. Genomic DNA was extracted from 20 D. kuriphilus adults for constructing polymerase chain reaction-free (PCR) Illumina 300-500 bp insert libraries and PacBio 20 kb insert library and sequenced on Illumina HiSeq 2500 and PacBio Sequel platforms, respectively. A total of 227.2 Gb Illumina clean reads and 336.5 Gb Pacbio long reads were generated in this study (Table 1), Illumina sequencing data quality assessment revealed that the quality of paired-end Illumina sequencing data is high, with the single-base error rate of over 91.5 % sequences being less than 0.001 (i.e., Quality scores 30; Figure 1A and Figure 1B). Furthermore, random sampling of 10,000 pairs of filtered high-quality data was conducted for sequence comparison with the National Center for Biotechnology Information (NCBI) nucleotide database (NT) using the Blast software to analyze microbial contamination in the sequencing data. The results indicate that the sequencing data mainly aligns with species closely related to chestnut gall wasps, accounting for approximately 90.7% of the total reads data. In addition, a small proportion of reads were found to align with the Wolbachia genome, representing approximately 0.01%. These results suggest that the Illumina sequencing data of chestnut gall wasps exhibit high quality and are free from microbial contamination. The PacBio sequencing depth analysis revealed an average read length of 19.5 Kb, an N50 length of 28.5 Kb, and an N90 length of 10.8 Kb, indicating high-quality PacBio sequencing.