GSM6480479: SmcHD1-KO JB4/EI7HZ2 NSCs, scRepli-seq, S 011; Mus musculus; OTHER
Single cells were sorted with a Sony SH800 cell sorter. Sample preparations were performed as described (Miura et al., 2020). DNA samples were subject to whole-genome amplification (WGA) with a SeqPlex kit (Sigma, SEQXE). NGS libraries were constructed WGA DNA with an NGS LTP Library Preparation Kit (KAPA, KK8232) according to the manufacturer's instructions