GSM7854247: WT_KM_3; Danio rerio; RNA-Seq
For scRNA-Seq analysis of eosinophils from kidney, eosinophils were sorted from adult Tg(eslec:eGFP) kidneys. For scRNA-Seq analysis of renal cells of different genotypes, kidneys were collected from WT, cebp1-/-, and cebpb-/- individuals and prepared into cell suspensions. The cell suspensions were then applied to 10x 3' scRNA-Seq GEM generation. Post GEM generation, the library construction was performed strictly based on the manufacturer's instructions (10x Genomics, USA).