RNA-seq of mouse: E12 Kdm3a KO NSPC
Total RNA was extracted using TRIzol reagent (TaKaRa, 9109), andmRNA samples were prepared for the RNA-seq analysis using the Illumina TruSeq RNA SamplePrep Kit V2 (Illumina, San Diego, CA, USA). The cDNA libraries were constructed by ligatinguniversal adapters to the cDNA fragments and performing PCR amplification.