RNA-Seq of Meles meles: intestine
RNA integrity was assessed using the 2100 Bioanalyzer system and the RNA 6000 NanoTotal RNA Assay (Agilent Technologies, Santa Clara, CA, USA). Double stranded cDNA was synthesized using Maxima H Minus Double-Stranded cDNA Synthesis Kit (Thermo Fisher Scientific, Waltham, MA, USA) according to manufacturers instructions. Sequencing libraries were produced using Nextera XT v2 Library Preparation kit (Illumina, San Diego, CA, USA) or Celero DNA-Seq library preparation kit (NuGEN, Redwood City, CA, USA) according to manufacturers recommendations, and were sequenced in 250-paired end mode on the Miseq or NovaSeq 6000 platforms (Illumina, San Diego, CA, USA), respectively.