RNA-seq of organismal: microbiome
Marine bacteria were targeted using the prokaryote 16S ribosomal RNA (rRNA) gene from the V4 region, using an updated version of the primer set 515F (GTGYCAGCMGCCGCGGTAA) and 806R (GGACTACNVGGGTWTCTAAT). PCRs, amplicon purification, library prep, and 16S sequencing with the Illumina Miniseq MO 2x150 occurred at the Genome Research Center at the University of Illinois-Chicago.