GSM8155262: PHHs, scRNAseq; Homo sapiens; RNA-Seq
Fresh uncultured PHHs were lysed and the released RNA was barcoded through reverse transcription in individual gel beads in emulsion (GEMs). Reverse transcription was performed on a S1000 Touch Thermal Cycler (Bio-Rad) at 53 °C for 45 min, followed by 85 °C for 5 min, and then held at 4 °C. The cDNA was generated and then amplified, and quality-assessed using the Agilent 4200 system. According to the manufacturer's instructions, scRNA-seq libraries were constructed using the Single Cell 3′ Library and Gel Bead Kit V3.1.