GSM7156147: SIRT2 knockout, control [T14]; Mus musculus; RNA-Seq
Advanced molecular biology equipment was used to detect the purity, concentration and integrity of RNA samples to ensure the use of qualified samples for transcriptome sequencing. Eukaryotic mRNA was enriched by magnetic beads with Oligo (dT). mRNA was randomly interrupted by Fragmentation Buffer. The first cDNA strand was synthesized by random hexamers using mRNA as template. Then the second cDNA strand was synthesized by adding buffer solution, dNTPs, RNase H and DNA polymerase I. The cDNA strand was purified by AMPure XP beads. The purified double-stranded cDNA was end-repaired, A tail was added and sequencing connectors were connected. AMPure XP beads were used for segment size selection. Finally, cDNA library was obtained by PCR enrichment.