GSM6753221: HCE051_P; Homo sapiens; RNA-Seq
Biopsies were briefly washed with DMEM/15%FBS, minced, digested with dispase (5000 caseinolytic Units) followed by trypsin (0.25%). Single cell suspensions were washed in PBS/2% BSA and checked for viability > 85% 10X Genomics NEXT GEM Kit V2, 10X genomics Library Construction kit