GSM7688434: no_nic_T1_R2; Paenarthrobacter nicotinovorans; RNA-Seq
Native RNA was extracted with the Promega SV Total RNA Isolation System (after 8.C., step 7 were added 8 µl of Proteinase K 20 mg/ml (Promega) and samples were incubated at 37⁰ C for 10 min before proceeding with 4.E, Steps 1–9). Native RNA was polyadenylated using the Lucigen Poly(A) Polymerase Tailing Kit. Library preparation was performed with the Oxford Nanopore Technologies (ONT) Direct RNA sequencing kit (SQK-RNA002) and an adapted protocol for the MinION Flongle flowcells (https://www.protocols.io/view/flongle-directrna-library-preparation-kqdg35847v25/v1 ).