GSM6753252: LIC, input; Mus musculus; RIP-Seq
Cells were crosslinked by UV (Stratalinker 1800 (254nm),120mJ/cm2 ×3) . After cell lysis, chromatin was sonicated using a ultrasonicator (Bioruptor). The purified mRNA fragments were used to construct libraries with TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA),1 μg of RNA was used as starting material for input and ip samples. Libraries were amplified using 20 cycles on the thermocycler. Post amplification libraries were size selected at 250-450bp in length.