RNA-Seq of FACS-sorted ex vivo NK cells from control (Cre neg, GFPNK/NK), STAT5BNK/NK, N642HNK/NK and diseased N642HNK/NK mice
RNA-Seq of FACS-sorted ex vivo NK cells from control (Cre neg, GFPNK/NK), STAT5BNK/NK, N642HNK/NK and diseased N642HNK/NK mice
Frozen liver samples from aged non-diseased Cre neg (n=3), GFPNK/NK (n=2), STAT5BNK/NK (n=3) and N642HNK/NK (n=5) mice and from diseased N642HNK/NK mice (n=8) were thawed and (GFP+)CD3-NK1.1+ NK cells were directly sorted into a SMARTSeq lysis buffer with a CytoFlex SRT Sorter (Beckman Coulter).  This was part of the library construction protocol. Libraries were constructed using the SMART-SEQ3 method (Michael Hagemann-Jensen, et al.; Nat Biotechnol. 2020 Jun;38(6):708-714).