GSM7157135: JHU083, TAMs, mouse 955; Mus musculus; RNA-Seq
Total RNA was extracted from sorted tumor associated macrophages (Live CD45+CD3-Ly6G-CD11b+F4/80+) with Trizol (Thermofisher) based on manufacturer's protocol. RNA was converted to cDNA with the Ovation RNA-seq System v2.0 kit and library construction was performed with the Truseq Nano kit (Illumina).