RNA-Seq of streptococcus mutans biofilm treated by NaF
The RNA library construction was performed as described in the TruSeqTM RNA Sample Preparation Kit (Illumina), including mRNA purification, mRNA fragmentation, cDNA synthesis and modification, library enrichment and sequencing. After amplification, Picogreen dsDNA (TBS380) Kit was used to test the dsDNA concentration and remove single stranded DNA. The Agilent 2100 bioanalyzer System was used to test the sample size and purity.