GSM8031218: Ctr biol rep 2; Nicotiana benthamiana; RNA-Seq
Total RNA was extracted from N. benthamiana inoculated leaves at 4 and 7 days after inoculation and from upper leaves 7 dpi as described (García-Marcos et al., 2013). RNA libraries for RNA-seq were performed at Novogene in United Kingdom. The integrity and quality of the total RNA were checked using NanoDrop 2000 Spectrophotometer. mRNA was purified from total RNA using poly-T oligo-attached magnetic beads.