S15_ChIP
anti-Histone H3 (di methyl K9) ChIP Grade ab1220 (Abcam), anti-Histone H3 (tri methyl K9) ChIP Grade ab8898 (Abcam), and RNA pol II antibody 39497 (Active Motif) antibodies were used to immunoprecipitated the DNA. According to their isotypes, primary ChIP antibodies were conjugated to protein A or G magnetic beads before DNA purification. Samples were washed and decrosslinked following a previously established procedure, and DNA was purified and eluted using a phenol:chloroform extraction method. IP and Input DNA samples were used to prepare libraries with Roche KAPA HyperPrep Kit, loaded into an Illumina NovaSeq 6000 S Prime flow cell and sequenced to obtain 100-bp paired-end reads. H3K9me3 and RNA pol II share the same Input DNA sample, while H3K9me2 has its own separated Input DNA sample.