HEK293T Cells treated with METTL3 inhibitor Chr1
HEK293T cells were grown using DMEM supplemented with 10% FBS and 1% penicillin-streptomycin. Cells were treated with 20 M STM2457 and incubated for 24 hours at 37C. Total RNA was extracted from 10 million cells using Qiazol (Qiagen 79306) and RNeasy Micro Kit (Qiagen 74004). mRNA purification was performed with 100ug of Total RNA using MACS mRNA Isolation Kit (Miltenyi Biotec 130-075-201) following the manufacturer's protocol. 150ng of mRNA were used as an input for Nanopore Direct RNA sequencing libraries preparation. 120ng of library were obtained and loaded in a PromethION flow cell with 8233 active pores for sequencing.