GSM6807215: R22035325LR02-RKO-shPNP-4G-4h; Homo; RNA-Seq
mRNA was extracted from total RNA using magnetic beads with oligo-dT then were fragmented randomly.  The first strand of cDNA and the second strand are then synthesized using reverse transcriptase. The reverse transcription product is subjected to terminal repair, followed by addition of an A base at the 3' end. Subsequently, the fragments were ligated with a Illumina universal adapter. After PCR amplified, the ligation products were purified in order to remove the incompletely ligated fragment and the self-ligated adapter. Library sizes were detected using an Agilent 2100 Bioanalyzer and library concentrations were detected using Qubit 2.0. The RNA sequencing was performed according to Illumina's standard protocol.