VK7-highly resistant replicate 3
Whole legs from 3 to 5day old, non-blood fed female mosquitoes were dissected including all leg segments (coxa, trochanter, femur, tibia, and tarsus), using microdissection forceps (Fig. S2). Four biological replicates each including legs from 20 to 30 female mosquitoes were prepared from each strain/condition. All collections were carried out between 1 and 3h after beginning of dawn period. RNA extraction was done using the Arcturus PicoPure RNA Isolation Kit (Thermo Fischer Scientific), coupled with RNase-Free DNase Set (QIAGEN).