AFLPseq of Pedicularis rostratospicata Crantz subsp. marsica A1293
CTAB DNA extraction; AFLP genome-reduction: Restriction/Ligation reaction (Mse + EcoRI), preselective PCR, selective PCR; Barcode PCR; equimolar pooling with other library samples; size-selection by gel excision; Ligation by Sequencing LSK109 protocol by ONT; MinION Flongle Nanopore sequencing; Basecalling (guppy_basecaller) and demultiplexing (guppy_barcoder)