RNA seq of mus musculus Therapeutic mice liver
The animals were segregated into three experimental groups: Control mice that were fed Normal pellet diet, T2DM mice that were fed High Fed diet followed by Streptozotocin treatment to induce diabetes (T2DM) condition, another group of T2DM mice was intravenously administered PCK-1 siRNA conjugated to GPR8. The animals were sacrificed after 28 days and liver tissues of respective experimental groups were used for RNA sequencing and to study the cross talk of PCK-1 silencing on different hepatic pathways.