GSM6481219: RepSeq_RKO_IDH2_WL_rep3; Homo sapiens; OTHER
Cells were then lysed and genomic DNA extracted using Zymo Quick-DNA Microprep Kit. DNA was fragmented using a Covaris E220 ultrasonicator and genomic libraries generated using the NEBNext Ultra DNA Library Prep Kit. Fragments were then immunoprecipitated with primary mouse anti-BrdU and secondary rabbit anti-mouse antibodies. Libraries were amplified with NEBNext Multiplex Oligos for Illumina. Libraries were size-selected with AmpureXP beads and quality control performed with Tapestation (Agilent), Q-bit (ThermoFisher) and KAPA quantification (Roche).