GSM7916346: HEK-293T, HS2-HBD_treated_by_ABA; Homo sapiens; OTHER
Cells was fixed by 1% Formaldehyde and chromatin was sonicated after cell lysis, nuclei permeabilization, is situ digestion & ligation. Sequencing libraries were constructed following the established in situ ChIA-PET protocol using 10 million input HEK 293 cells co-expressing both ABI1-mCherry-dCas9-VP64-FUS with gHS2 and PYL1-BFP-dCpf1-VP64-FUS with crRNAs before and after ABA treatment, employing an antibody specifically targeting mCherry