GSM8207340: lexA3 sspA∆, AZT-treated biol rep 2; Escherichia coli K-12; RNA-Seq
Total RNA was extracted using the Split RNA Extraction Kit (Lexogen) followed by RiboCop rRNA Depletion for Gram - Bacteria (Lexogen). Library preparation employed the CORALL2 library kit  and protocol (Lexogen). SIRV and ERCC RNA Spike-In Mix 1 (ThermoFisher) were added for quality control.  Unique Molecular Identifiers were employed to allow removal of PCR duplicates during read processing.