Shotgun-seq of freshwater: WLB 8m
Genomic DNA was extracted using a FastDNA SPIN kit (MP Biomedicals, Illkirch, France) after cutting the filter paper into several pieces. DNA libraries for sequencing were prepared with a TruSeq Nano DNA Library Prep Kit (Illumina, USA) following the manufacturer's instructions. Shotgun metagenomic sequencing was conducted by LAS Inc. (Gimpo, Korea) using the Illumina MiSeq platform (300 bp paired-end).