Amplicon MiSeq of glacier surface bacterial community
Community genomic DNA was amplified by PCR (New England Biolabs M0486 supplemented with molecular grade BSA, B9200) with 515F-806R primers with EMP-barcodes appended to 515F. All PCR products were pooled and cleaned by PCR purification kits (Qiagen, Inc.) before sequencing at the Cardiff University Genome Hub MiSeq using 2250 bp reads.