GSM8155177: Hepatoblastoma sample 19; Homo sapiens; OTHER
Ten tissue sections with a maximum area of 6.5 x 6.5 mm2 were cut from ten distinct HB FFPE tissue bocks, placed on the mRNA capture areas of Visium Gene Expression slides for FFPE (10x Genomics), fixed in methanol, and H&E stained according to the respective user guide (CG000409 Rev C, 10x Genomics). Brightfield histology images were taken using a 4x or 10x objective on a Nikon Eclipse Ni. Raw images were stitched together using NIS-Elements (Nikon) and exported as TIFF files with high-resolution settings. Libraries were prepared following the manufacturer's user guide (G000407 Rev D, 10x Genomics).