GSM6753902: differentiation of hIPSC line AD3 to dopaminergic neurons day 7 SH condition; Homo sapiens; RNA-Seq
Cells were washed by PBS and isolated using ExtractRNA reagent (cat. # BC032, Eurogen) All procedures were performed according manufacture's recommendations. Quality of RNA was checked with fluorimetric analysis by Quantus and capillary electrophoresis by QIAxel. PolyA RNA was purified with Dynabeads mRNA Purification Kit (Ambion). Libraries were made from polyA RNA with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) according to the manual. Single-read sequencing was performed on Illumina HiSeq2500 with 140 bp read length, with at least 30 million reads generated for each sample.