GSM7611089: Kupffer cells, wild type, hfdcdcd, repl 2; Mus musculus; RNA-Seq
Adult mice were anesthetized and perfused with ice-cold PBS. For livers, 300 mg were collected, cut into small pieces, and incubated with 1 ml of double-concentrated digestion mix (PBS containing 1 mg/ mlcollagenase D (Roche), 100 U/mlDNase I (Sigma-Aldrich), 2.4 mg/ mlof dispase (Invitrogen) and 3% fetal calf serum (FCS) (Invitrogen) for 30 min at room temeprature before the mechanical disruption 100-μm filter.  The cell suspension was diluted in 3ml of FACS buffer (0,5% BSA, 2mmol EDTA in PBS).  Cells were stained by antibody mixes and sorted using FACS ARIATM III cell sorter (BD Biosciences) directly into 500ul QIAzol lysis buffer (Qiagen). SmartSeq2 protocol