GSM7622014: EXOSC2 AID ESCs, RNA, 4 hr, rep2; Mus musculus; RNA-Seq
Total RNA were extracted by TRIzol reagent according to manufacturer's instruction. Purified total RNA were subjected to ribosomal RNA depletion by Ribo-Zero rRNA removal kits (Illumina) according to manufacturer's instruction. RNA-seq libraries were constructed by NEBNext Ultra II directional RNA library prep kits (NEB) following manufacturer's protocols.