miRNA-seq of mouse liver
At 2 weeks of age, both male and female mice (n=10 per group) were separated into: (1) vehicle saline-treated controls, (2) 100mg/kg metformin (MF) treated, and (3) 2mg/kg trodusquemine (MSI-1436) treated. Mice were administered either daily intraperitoneal (i.p.) injections of MF or triweekly i.p. injections of MSI-1436 for a period of six consecutive weeks. At 8 weeks (2 months) of age, mice were sacrificed and liver tissues were collected for miRNA sequencing