GSM7774582: H1, WT, rep1; Homo sapiens; RNA-Seq
Total RNA was extracted with TRIzol™ (ThermoFisher), 500 ng RNA was used for sequencing library construction. polyA RNA was enriched with NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs), then corresponding RNA libararies were prepared with NEBNext® Ultra II RNA Library Prep Kit for Illumina® (New England Biolabs).