S 4
50 ng of Qubit quantified DNA was enzymatically fragmented, end-repaired and A-tailed in a one-tube reaction using the FX Enzyme Mix provided. The end-repaired and adenylated fragments were subjected to adapter ligation, whereby index-incorporated Illumina adapter was ligated, to generate sequencing library. The library was subjected to 6 cycles of Indexing-PCR (Initial Denaturation at 98C for 2 min, cycling (98C for 20sec, 60C for 30sec, 72C for 30sec) and final extension at 72C for 1min) to enrich the adapter-tagged fragments. Finally, the amplified libraries were purified using Sera-MagTM Select beads followed by library quality control check.