GSM6605601: Ctlab-16wk-Tdtneg1; Mus musculus; RNA-Seq
The aortic root and ascending aorta were excised and washed three times in PBS. Collected tissues were placed into an enzymatic dissociation cocktail in Hank's Balanced Salt Solution (HBSS)) and minced. After incubation at 37 °C for 1 h, the cell suspension was strained, pelleted by centrifugation. Cells were loaded into a 10X Genomics microfluidics chip and encapsulated with barcoded oligo-dT-containing gel beads using the 10X Genomics Chromium controller according to the manufacturer's instructions. Single-cell libraries were then constructed according to the manufacturer's instructions. Libraries from individual samples were multiplexed into one lane prior to sequencing on an Illumina platform with targeted depth of 50,000 reads per cell.