0.3uM multiplexNanoRanger of 293T
The native DNA and the products thereafter were quantified using Qubit. Sample products were barcoded and library-prepared using the ONT Ligation Sequencing Kit (SQK-LSK109) and Native Barcoding Expansion 1-12 Kit (EXP-NDB104) following the manufacturers instructions (available from https://community.nanoporetech.com/protocols), expect that all elutions were done for 10 min at 37C. For each library, approximately 50 fmol was loaded onto a Release 9.4.1 flow cell for sequencing on an ONT MinION sequencer.