Element sequencing of HiC library of adult male Bactrocera oleae UH_Lalamilo Wild
Tissue was cross-linked, digested using the enzymes DdeI and DpnII, and proximity ligated. After proximity ligation and nucleic acid purification, DNA was sheared using the Diagenode Bioruptor Pico and size-selected to enrich for DNA fragments 200-600 bp. Following fragmentation and sample purification using SPRI-beads, library preparation was performed using the NEB Next Ultra II DNA Library Prep Kit and followed by the Element Biosciences Adept Library Prep Kit. The resulting HiC library was sequenced on one flow cell of an Element Biosciences Aviti system to 100x coverage of the genome size.