GSM7156948: #4304 donor, HBB-SNP WX 2500 MOI, pIM-313 HDR template rep3; Homo sapiens; OTHER
high molecular weight genomic DNA was extracted, and 5' and 3' phosphates were removed. The b-globin gene cluster was enriched by Cas9-RNPs digestion of two 10 kb fragments. PacBio SMRTbells were ligated to fragments, pooled and sequenced on a PacBio Sequel IIe PacBio libraries were generated using the No Amp protocol