Mantle transcriptome normal pH (8.0) Replicate 2
After relaxation, mantle tissues were dissected and stored immediately at -80C until further RNA extraction. Total RNA was extracted from mantle tissues of adult oysters kept under normal and low pH conditions with TriReagent (T9424, Sigma) following a modified protocol described by Gao et al. (2001) to remove inhibitory pigments. RNA was quantified using a NanoDropTM Lite Spectrophotometer (Thermo Scientific) and shipped to Macrogen (Korea) to perform cDNA library preparation and sequencing in an Illumina NovaSeq6000 paired-end mode.