GSM7106664: CAFs Tumor 9; Mus musculus; RNA-Seq
tumors were harvested, dissocaited in collagenase, and cells lysed in buffer RLT Total RNA was extracted following the RNeasy Plus Mini kit (Qiagen) according to the manufacturer's protocol before being sequenced by the Genomics Research Core. Quality control was determined by RNA concentration using the NanopDrop 1000 Spectrophotometer (NanoDrop), and RNA quality was measured using the Agilent Bioanalyzer 2100 (Agilent). The SMARTer Ultra Low Input kit v4 (Clontech) was used to preamplify 1 ng of total RNA, and the resulting cDNA product was assessed for quality and quantity using the Qubit Flourometer (Life Technologies) and the Agilent Bioanalyzer 2100 (Agilent). Sequencing libraries were generated using 150 pg of cDNA and the NexteraXT library preparation kit (Illumina) before being amplified and hybridized to the Illumina flow cell and sequenced using the NovaSeq 6000 sequencer (Illumina) on the S1 flow cell. Approximately 25 million reads were collected per sample and single end reads of 100 nt were generated.