GSM8253652: MC-LR-Extracellular Vesicles2; Homo sapiens; ncRNA-Seq
700 μL QIAzol was added into exoexotic body weight suspension and swirled for 10s-Centrifuge briefly and incubate at room temperature for 5min-Add 140μL chloroform/isoamyl alcohol (24:1), violently upside down for 15s, and incubate at room temperature for 2~3min-Centrifuge at 4℃ at 12,000×g for 8min. Drain the supernatant into the new tube-Add two times the supernatant volume of anhydrous ethanol, mix well-All the mixture was purified by column-Add 700μL RWT buffer to wash once-Add 500μL RPE buffer and wash twice-Centrifuge 12,000×g for 2min to dry the film and discard the collection tube-The purification column was moved to a new collection tube and 20μL RNA-Free water was added. The RNA was incubated at room temperature for 1min, and centrifuged at 12,000×g for 2min to eluate RNA, and about 15μL eluted product was obtained. RNA libraries for RNA-seq were prepared using DNBSEQ According to following manufacturer's protocols.