GSM8365513: ChiPSC12, ETV6::RUNX1, clone 1,scRNA-seq; Homo sapiens; RNA-Seq
Following in vitro differentiation of hiPSCs, hematopoietic progenitor cells of 5 wells were pooled, filtered and resuspended in PBS with 0.04% BSA (Biowest). Cell viability was determined to be >70% using the BD Rhapsody Single-Cell Analysis system (BD Biosciences). Single-cell suspensions were used for single-cell droplet library generation on the 10X Chromium Controller system using the Chromium Single Cell 3' NextGEM Reagent kit v3.1 (10X Genomics) according to the manufacturer's instructions.