16S rRNA sequencing of mouse feces
Microbial DNA was extracted using a FastDNATM 2 mL SPIN Kit (MP Biomedicals, Santa Ana, CA) in combination with a Precellys24 homogenizer (Peqlab, Erlangen, Germany). The V1-V2 region of the bacterial 16S rRNA gene was amplified with primers 27F and 338R, linked with double index (8nt on each of the primers), and then processed on Illumina MiSeq sequencer with MiSeq Kit V3 (300bp paired-end).