SO4 Ch 96 well plate incubation t2 5e-6 rep1
Sediment incubation DNA was extracted using the Beckman DNAdvance kit (A48705). 16S rRNA gene amplicon sequencing of the V4 region was carried out using the Earth Microbiome Project protocol (Caporaso et al., 2011; Caporaso et al., 2012) with the 515F/806R primer set (515F 5' - GTGYCAGCMGCCGCGGTAA; 806R 5' - GGACTACNVGGGTWTCTAAT).