GSM8253714: Untreated, replicate 2, scRNA-seq; Homo sapiens; RNA-Seq
Cells were harvested from T75 flasks, washed twice with 1x phosphate-buffered saline (PBS) (Gibco 10010049), and filtered using a Falcon strainer (Falcon 100-0087). Cell viability was assessed using an automated cell counter with trypan blue staining. Subsequently, cells were diluted to a concentration of 5 × 105 cells/ml in PBS, and dead cells were labeled with Propidium iodide (PI, 3 μM). Single cells were isolated using the BD Influx cell sorter before being loaded onto the 10X Chromium Controller. The single-cell RNA-seq (scRNA-seq) library was prepared following the Chromium Single Cell 3' Reagent Kits User Guide (V3 Chemistry) from 10X GENOMICS.