GSM7611058: S534A-2; Homo sapiens; RNA-Seq
Total RNA was isolated using Trizol/Chloroform extraction method and RNA quality was validated on RNA-1000 chip using Bioanalyzer (Agilent). 1.0 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using NEBNext Ultra II Directional RNA Library prep Kit following manufacturer's protocols.