GSM7884675: SW1990_RNAseq_rep1; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol Reagent (Life Technologies, CA, USA) according to the manufacturer's protocol, and RNA integrity was assessed using an Agilent Bioanalyzer 2100 (Agilent Technologies, CA, USA) to obtain RIN values. RNA with RIN values > 7 was purified using an RNAClean XP Kit (Beckman Coulter, Inc. CA, USA) and RNase-Free DNase Set (QIAGEN, GmBH, Germany). RNA libraries were prepared for sequencing using a VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina (Vazyme, Nanjing, China), and sequencing was performed on an Illumina HiSeq 2500 system.