Illumina HiSeq 2000 paired end sequencing; ChIP-seq of mouse primary cultured bone marrow-derived neutrophils stimulated by lung cancer cell conditional medium
ChIP-seq of mouse primary cultured bone marrow-derived neutrophils stimulated by lung cancer cell conditional medium
Bone marrow cells extracted from C57BL/6J mice were treated with 10% LLC1 conditional medium or normal medium as the control for 2 hours, then processed using the SimpleChIP Enzymatic Chromatin IP Kit according to the manufacturerâ€™s instructions. Antibodies against Smad3 and IgG isotype were used for immunoprecipitation and DNA enrichment. We performed ChIP-seq for smad3 immuno-enriched DNA (Input and Smad3-IP) using the Illumina HiSeq platform (PE150bp, 40M raw read) and mapped clean sequences against GRCm38 using Bowtie2, and peaks were identified using model-based analysis for ChIP-Seq (MACS) with default parameters.