GSM7695979: Control, Lbr and Lmna Barcode Library; Mus musculus; RNA-Seq
Adult retinas were dissected and placed in StemPro Accutase (Gibco) for 30 minutes at 37 °C. Cells were triturated manually, incubated with Dapi as a viability marker, and then sorted by the OHRI Flow Cytometry and Cell Sorting Facility using a Beckman Coulter MoFlo XDP. After flow cytometric sorting, cells were barcoded with 'anchor' and 'co-anchor' lipid-modified oligonucleotides generously provided by the Zev Gartner lab. Barcode oligonucleotides were purchased from Integrated DNA Technologies as follows. Barcode 1: F: 5′- CCTTGGCACCCGAGAATTCCAGGAGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA-3′; Barcode 2: F: 5′- CCTTGGCACCCGAGAATTCCACCACAATGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA-3′; Barcode 3: F: 5′- CCTTGGCACCCGAGAATTCCATGAGACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA-3′;  Each replicate was incubated with barcode oligonucleotides for 10 minutes. Cells were pelleted and washed 3 times with PBS. Replicates were pooled and processed in a single 10X Genomics Chromium run.