GSM7621967: HeLa-TREx_EMPTY_VECTOR clone 12.2 (REP3); Homo sapiens; RNA-Seq
Total RNA was extracted using Trizol (Thermofisher cat. 15596026); 4sU-labelled RNA was extracted as described (Austenaa et al. 2015, PMID 26593720) Library preparation was performed using the TruSeq Stranded Total RNA sample preparation kit (Illumina RS-122-9007) using 80-200 ng of 4sU-labelled RNA, without ribosomal depletion.