RNAseq of the white seafan Eunicella singularis
Polyps were dissected from the colony under a stereomicroscope and pooled in three aliquots of about 20 polyp each (corresponding to about 15 mg of tissue) that were preserved in Trizol at -80 C until further processing.Total RNA was extracted using Trizol (Thermo Fisher Scientific), following the manufacturers instructions,obtaining 5-10,000 ng with RQN values between 6.7 and 8.8. The three libraries were then processed for paired-end sequencing-by-synthesis inan Illumina HiSeq 2500 at the MGX facility (Montpellier, France), and quality controlled using FastQC. Aftertrimming, we obtained a number of reads longer than 30 bp and quality above Q20 comprised between 21and 30 million.