GSM6656385: Blood, CD4, TEM, 08; Homo sapiens; RNA-Seq
Sorted cells were lysed in buffer RLT (QIAGEN) plus 1% β-mercaptoethanol and stored at -80°C until further processing. Total RNA was isolated with the RNeasy micro kit (QIAGEN) according to the manufacturers' instructions. PolyA-enrichment and NebNEXT low input library prep kit