GSM7612709: 214N-Primary; Homo sapiens; RNA-Seq
RNA samples were extracted using RNeasy plus mini kit (Qiagen, Hilden, Germany) to remove genomic DNA. 50ng RNA samples at 5ng/ul concentration were reverse-transcribed by poly-A selection using oligo dT primer. Clontech SMARTv4 (Takara Bio) and Nextera XT (llumina) kits are used to construct the library according to the manufacture's protocol. The sample pool was sequenced at 30 million 50PE reads per sample, Nextseq P2 flowcell.