RNA-seq of Phaseolus vulgaris roots transformed with PHR1 Ox and optimal Pi condition
RNA sample integrity and concentration were determined using an Agilent 2100 Bioanalyzer (Agilent Genomics, Santa Clara, CA, USA), at the Unidad de Secuenciacin Masiva, Instituto de Biotecnologa, UNAM. RNA-seq libraries were prepared following the manufacturers instructions from isolated total RNA using the Illumina TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA, USA). The libraries were sequenced using the manufacturers protocol of the Illumina NextSeq 500 platform for 75 paired-end cycles.