GSM7855458: ftdc2_wt_m2_re2; Mus musculus; RNA-Seq
The zona pellucidae of oocytes and blastocysts were removed with 0.5% pronase E (Sigma). 14 oocytes per sample (2 samples for each group) and 5 blastocysts per sample ( 2 samples for each group) were lysed and subjected to first strand synthesis. The single-strand RNA molecules were reversed with oligo (dT) primer. Amplified cDNA was fragmented into ~300 bp fragments and then constructed by TruePrep DNA Library Prep Kit (Vazyme).