shotgun metagenomics of pediatric stool sample
Shotgun metagenomic sequencing libraries were prepared with Illuminas Tagmentation Protocol with the Illumina DNA Prep Kit as per the manufacturers instructions. DNA fragments were then barcoded with NexteraTM DNA CD Indexes (Illumina). Library concentrations were determined with PicoGreen and samples were pooled for sequencing. Pooled libraries were sequenced on a NextSeq 500 sequencing platform (Illumina).