GSM8156169: RNA, islets, Clec control RFD biol rep2; Mus musculus; RNA-Seq
For both islets and liver: Total RNA was isolated from cultured islets or frozen liver samples and DNase treated using commercially available kits (Omega Biotek and Ambion, respectively). Libraries were constructed and subsequently subjected to 151 bp paired-end cycles on the NovaSeq-6000 platform (Illumina). FastQC (v0.11.8) was used to ensure the quality of data. RNA-sequencing was performed by the Advanced Genomics Core at University of Michigan Medical School.