GSM8068085: ATAC_NTC biol rep 2; Mus musculus; ATAC-seq
5x10e4 D2A1-d cells were prepared and collected. Cells were then washed once with cold 1xPBS and spinned down at 500g for 5min at 4C. Cells were kept on ice and subsequently resuspended in 25ul 2xTD buffer (Illumina Nextera kit), 2.5ul Transposase enzyme (Illumina Nextera kit, 15028252) and 22.5ul Nuclease-free water in total of 50ul reaction for 1hr at 37C. DNA was then purified using Qiangen MinElute PCR purification kit (28004) in a final volume of 10ul. ATAC-seq Libraries were prepared following the Buenrostro protocol (http://www.ncbi.nlm.gov/pmc/articles/PMC4374986/)