RNA-Seq of river water (Havel, Germany)
100 ng was introduced into Illumina's TruSeq stranded mRNA library preparation kit. In order to address all RNA molecules (not only poly-adenylated RNA), the protocol was adapted as follows: RNA was precipitated using isopropanol and resolved in Fragment, Prime, Finish Mix (FPF). From this step on, the manufacturer's protocol was followed (p20, step 12, TruSeq Stranded mRNA Sample Preparation Guide, Part # 15031047 Rev. E, Illumina).