RNA-seq of human cell line with different genotype
Total RNA was extracted using AllPrep DNA/RNA Kits (Qiagen 80204) and quantified with Qubit High Sensitivity RNA Kit (Life Technologies Q32852). Total RNA (1__g) was used to generate RNA-seq library with KAPA HyperPrep RNA Kits with RiboErase (Roche KK8560) using 8 cycles of PCR amplification. Libraires were validated on a Tapestation 4200 (Agilent G2991BA), quantified by Qubit High Sensitivity dsDNA Kit (Life Technologies Q32854), multiplexed and sequenced on a NovaSeq6000 using 100-10-10-100bp reads format.