16SrRNA of mus musculus: colon contents
Samples after different treatments were harvested from mice and frozen at -80  for 16S rRNA gene sequencing analysis. Briefly, microbial community DNA extracted by the CTAB method was amplified to generate sequencing libraries using TruSeq DNA PCR-Free Sample Preparation Kit (Illumina, USA) according to manufacturers recommendations, followed by sequencing the library on an Illumina NovaSeq platform.