HiFi reads of Corylus americana accession 'Rush' leaf tissue
High-molecular weight DNA was extracted following the protocol described by (Vaillancourt and Buell 2019).  In brief, this involved homogenizing leaf tissue in liquid nitrogen, lysis of homogenized tissue with Carlson lysis buffer, followed by purification using chloroform, and a final purification with Qiagen Genomic-tip.  Purity of extracted DNA was assessed spectrophotometrically using a NanoDrop One (ThermoFischer Scientific).  DNA was then quantified a using Qubit dsDNA High Sensitivity kit (ThermoFisher Scientific), and then diluted and assessed for size using an Agilent FemtoPulse System.  A Pacific Biosciences HiFi library was prepared according to PN 101-853-100 Version 03 (Pacific Biosciences). Modifications included shearing with a Covaris g-TUBE and size selecting with Sage Sciences BluePippin.  The library was sequenced on a Sequel II using Sequel Polymerase Binding Kit 2.2 at the University of Wisconsin  Madison Biotechnology Center DNA Sequencing Facility.