Illumina NovaSeq 6000 paired end sequencing
Sequencing libraries were prepared from 750ng total RNA using the TruSeq stranded mRNA library preparation kit (Cat# 20020594/5, Illumina Inc.) including polyA selection. The library preparation was performed according to the manufacturers' protocol (#1000000040498). NovaSeq S1 flowcell, paired-end 100bp read length, v1 sequencing chemistry.