Genomic sequencing of hMPXV: USAFSAM Public Health
Viral sequencing was performed following a tiling amplicon strategy Chen et al. at https://www.protocols.io/view/monkeypox-virus-multiplexed-pcr-amplicon-sequencin-5qpvob1nbl4o/v2. Sequencing was performed using a NextSeq 550 sequencing instrument. Libraries were prepared using the Nextera XT library kit.