RNA-seq of Xylanibacter ruminicola KHP1 culture grown in the absence of cobalamin, replicate 5
total RNA was extracted from ~4g of flash-frozen KHP1 culture in RNAprotect, followed by acid phenol-chloroform extraction with bead beating, DNase treatment (TURBO DNase), purification (MEGAclear). Total RNA samples were then sent to Novogene Ltd (Beijing, China) for rRNA depletion (RiboZERO Magnetic kit), library preparation (NEBNext Ultra II Directional RNA Library Prep kit) and sequencing (Illumina Novaseq 6000)