WGS of Macrophomina phaseolina: Glycine max
Libraries were prepared using the Illumina TruSeq Nano DNA Library Preparation Kit HT on a Perkin Elmer Sciclone G3 robot following manufacturers recommendations. Completed libraries were QCd and quantified using a combination of Qubit dsDNA HS and Caliper LabChipGX HS DNA assays. Based on these quantitations all 96 libraries were pooled in equimolar amounts and the pool quantified using the Kapa Biosystems Illumina Library Quantification qPCR kit. This pool was loaded on one (1) lane of an Illumina HiSeq 4000 flow cell and sequencing performed in a 2x150bp paired end format using HiSeq 4000 SBS reagents. Base calling was done by Illumina Real Time Analysis (RTA) v2.7.6 and output of RTA was demultiplexed and converted to FastQ format with Illumina Bcl2fastq v2.19.0.