OX of NUDT17-Flag, biological replicate 2
For each biological replicate, 3-4 plants were pooled and RNA was extracted using the RNeasy plant mini kit (Qiagen). 100 ng of total RNA per sample determined using a Qubit fluorometer (Thermofisher). RNA quality control using 2100 Bioanalyzer system (Agilent Technologies), library preparation and sequencing were performed by the iGE3 Genomic Platform at the Faculty of Medicine, University of Geneva (https://ige3.genomics.unige.ch/). Sequencing was performed with Novaseq 6000 machine from Illumina with 100 bp single-read output.