GSM7951836: Liver, male, 2B6, 10 PFOS, sample 159 [M_159_2B6_10]; Mus musculus; RNA-Seq
RNA extractions were performed on 20 mg of liver tissue previously stored at -80C. RNA was extracted with TRIzol (Life Technology, Carlsbad, CA USA) and chloroform. Quality was checked via Nanodrop (Thermo Fisher Scientific, Walkham, MA USA) to ensure both A260/280 and A260/230 values were about 2.0. Library construction was performed by Novogene (Sacramento, CA USA) using messenger RNA purified from total RNA. Quality was checked with Qubit (Thermo Fisher Scientific, Walkham, MA USA).