GSM6856507: old2; Homo sapiens; RNA-Seq
To isolate single cell suspension, we used Whole Skin Dissociation Kit (Miltenyi Biotec 130-101-540). We excluded enzyme P. We incubated skin biopsy for 15 hrs and stained with CD45 and propidium iodide and sorted CD45+ PI- cells. Immediately after sorting, we went to the scRNA seq. For scRNA seq step, we followed instruction of Chromium Next GEM single cell3 3' Reagent Kits v3.1 (10x Genomic). We used NextSeq 500/550 Hi Output Kit v2.5 150 cycle (illumina) for sequencing.