GSM7034384: HEL cells, shControl, PANDORA-seq, rep2; Homo sapiens; ncRNA-Seq
Total RNAs were extracted by Trizol (Invitrogen). Then small RNAs sized at 15-50nt were excised from 15% (w/v) urea polyacrylamide gel. Small RNA libraries were constructed according to NEBNext® Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs).