GSM7035757: HuH-7_meRIP_Input; Homo sapiens; RIP-Seq
Total RNA from HuH-7 cells were extracted using TRIzol reagent (Invitrogen, CA, USA) following the manufacturer's instructions. For the RIP-seq, the sequencing library was prepared deploying NEBNext Ultra Directional RNA Library Prep kit (New England Biolabs, USA) and sequenced on the Illumina HiSeq 3000 platform.  For the MeRIP-seq, the library was prepared by smart-seq method. Both the input samples without IP and the m6A IP samples were subjected to 150-bp, paired-end sequencing on an Illumina NovaSeq 6000 sequencer.