GSM8155222: NHEM, replicate4, RNAseq; Homo sapiens; RNA-Seq
Total RNA was isolated using the RNeasyMini kit (QIAGEN) or Direct-zol RNA Miniprep (ZYMO RESEARCH). RNA sequencing libraries were prepared using the NEBNext UltraTM RNA Library Prep kit for Illumina (NEB, USA) or VAHTS Universal V8 RNA-seq Library Prep Kit for NovaSeq (Vazyme, China) following the manufacturer's recommendations.