Genome Sequence of L. cardiaca H63T
Using the Promega Maxwell 16 systems, genomic DNA was isolated from strain H63T. PacBio sequencing, gDNA was fragmented to an average size of ~11kb. Sheared DNA was cleaned with SPRIselect beads followed by library construction using SMRTbell Express Template Prep Kit 2.0, which includes single-strand DNA overhang removal, DNA damage repair, End repair/A-tailing and barcoded overhang adaptor ligation. BluePippin size selection  The library pool was further purified with SPRIselect beads, then quantified with Qubit 4.0 fluorometer and assessed on Agilent Fragment Analyzer.