Long-read WGS of adult female Amblyomma americanum: salivary glands
Salivary glands from approximately 25 adult female Amblyomma americanum ticks were extracted and stored in 100 microliters of distilled water chilled over an ice bath. Genomic DNA was extracted from pooled salivary glands immediately after extraction (never frozen) using a Circulomics Nanobind Big Tissue Kit according to manufacturer instructions. PacBio SMRT bell prep kit 3.0 was used to prepare libraries using a SageElf for size selection rather than AMPure PB beads and megaruptor instead of megaruptor 3.0. for fragment generation. Fragments of 15-18 kilobases were targeted for library preparation and all other recommended guidelines were adapted from the PacBio SMRT prep kit 3.0 whole genome and metagenome protocol manual.