RNA-seq of 1 minute cold plasma treated Staphylococcus aureus ATCC 6538
The harvested cells from the treatment were mechanical lysed with bead-beating method in a Qiagen Tissuelyser II. Then, the cell lysate was subjected to total RNA extraction using Zymo Research Quick-RNA Fungal/Bacterial MiniPrep Kit and DNA was removed using DNase I. An Illumina TruSeq Stranded Total RNA sample preparation kit coupled with a ribosomal rRNA depletion were used for library preparation. The prepared library was quality checked via a TapeStation 2200. RNA-seq was conducted at PE150 mode in NovaSeq 6000.