GSM7621989: HCT116_siSYMPK_AUX_repl_1; Homo sapiens; RNA-Seq
Total RNA was extracted using Trizol (Thermo Fisher cat. 15596026); 4sU-labelled RNA was extracted as described (PMID 26593720) Library preparation was performed using the TruSeq Stranded Total RNA sample preparation kit (Illumina RS-122-9007) using 80-200 ng of nascent 4sU-labelled RNA, without ribosomal depletion.