GSM7855498: healthy gingiva 2; Homo sapiens; RNA-Seq
RNA-Seq fastq files were first processed by fastp, to remove adaptor and low-quality reads, and then aligned to the GRCh38 human genome assembly by STAR (v2.5.3a), followed by quantification with HT-seq (v0.9.0). The resulting gene count data were first quantile-adjusted, using a conditional maximum likelihood (qCML) model (estimate Common Disp function of EdgeR version v3.29.2). Then, differentially expressed genes between the experimental groups were identified by edgeR (v3.29.2), and further filtered for at least 1.5-fold changes. Gene ontology (GO) analysis was performed using Metascape (http://metascape.org/gp/index.html#/main/step1). STAR (v2.5.3a)