Illumina HiSeq 2000 sequencing; Transcriptomics of Arabidopsis thaliana wild-type and epimutants recovering from long-term heat stress
Transcriptomics of Arabidopsis thaliana wild-type and epimutants recovering from long-term heat stress
At least 50 seedlings were pooled from three independent petri dishes for RNA extraction. For treatment, 7 days-old seedlings were subjected to changes in temperature only during the light period. Long-term heat consisted of an entrainment treatment of 5 heat cycles (day 37C, night 21C, 12/12 h; final age 12 days). This was followed by 3 days of recovery at 21C in recovery samples (age at harvest 15 days). Total RNA was isolated from biologically duplicated samples using the the Plant-RNAeasy kit (Invitrogen), following provider's instructions. Libraries were prepared with the corresponding Illumina library preparation kit, following providerâ€™s instructions.