GSM6807281: Mix5_a; Homo sapiens; RNA-Seq
Cell pellets of approximately 500K cells were prepared in triplicate and flash frozen at the time of the experiment. RNA was extracted using the Qiagen RNeasy Plus mini kit with a genomic DNA elimination column (74104). Extracted RNA samples were submitted to Novogene for library preparation and sequencing. Briefly, RNA quality was confirmed, 150bp paired-end mRNA libraries were prepared, and libraries were sequenced at a depth of 20M reads per sample on the NovaSeq 6000 platform (Illumina).