GSM7035401: PPA_M2; Mus musculus; RNA-Seq
Individual mouse tumors were enzymatically dissociated using collagenase, hyaluronidase, and DNAse I. Live CD45+ and CD3+ cells from the dissociated single-cell suspensions were stained and flow sorted to co-load onto Seq-Well arrays. CD45+ (whole immune) and CD45+CD3+ (T cell) single-cell suspensions from each tumor were loaded in a 1:1 ratio onto Seq-Well arrays and processed using the second-strand synthesis protocol described in Hughes et al., Immunity (2020) and Gierahn et al., Nature Methods (2017).