GSM6753469: blood MHCIIhi, rep1; Mus musculus; RNA-Seq
For RNA extraction, a 1% agarose gel was used to detect RNA degradation and contamination. Sequencing libraries were generated using the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, E7530L) and index codes were added to the attribute sequences of each sample and sequenced using the TruSeq PE Cluster Kit v3-cBot-HS (Illumina, PE-401-3001) on the cBot Cluster Generation System to cluster the index-coded samples.