GSM6606161: E14 TKO cells, 96H, biological replicate 2 (BulkRNAseq_PGCLC); Mus musculus; RNA-Seq
RNA extraction was performed on cell pellets using standard Trizol (Life Technologies)-chloroform protocol. RNA pellet was treated with DNase before clean up with RNeasy Mini kit (QIAGEN). RNA-seq libraries were prepared from a minimum of 10ng of DNase-treated total RNAs SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian