GSM8014184: Ath, protoplast, GmWRI1, br1; Arabidopsis thaliana; RNA-Seq
Total RNA was isolated using the RNAqueous-Micro total RNA isolation kit (Life Technologies) following the manufacturer's instructions. RNA-Seq libraries were prepared using Tecan Ovation RNA-Seq System V2 using 50 ng of total RNA, and double-stranded cDNA was fragmented to ~200 bp using a Covaris E220.  End repair, A-tailing, adapter ligation and PCR enrichment were performed according to Kumar et al. (Kumar R, Ichihashi Y, Kimura S, Chitwood DH, Headland LR, Peng J, Maloof JN and Sinha NR (2012) A high-throughput method for Illumina RNA-Seq library preparation. Front. Plant Sci. 3:202. doi: 10.3389/fpls.2012.00202) Supplemetary Methods 1 library preparation protocol, using NEXTflex ChIP-Seq Barcodes (BiooScientific).