Isoseq of Camelina sativa: developing seed
The Reverse transcription and cDNA libraries were prepared following the Pacific Biosciences Preparing Iso-Seq libraries using SMRTbell prep kit 3.0 protocol.  The DNA Damage repair, End repair and SMRT bell ligation steps were performed as described in the template preparation protocol (PN# 102-396-000) with the SMRTbell Express Template Prep Kit 3.0 reagents (Pacific Biosciences, Menlo Park, CA, USA).  .  The libraries went through a SMRTbell bead cleanup (following the SMRTlink v11.0 calculator procedure) before being sequenced on a PacBio Sequel II instrument at a loading concentration (on-plate) of 120pM using the adaptive loading protocol, Sequel II Sequencing Kit 2.0, SMRT Cell 8M and 24 hours movies with a 2h pre-extension time. The data processing was done using the SMRT Link Analysis V11.0.0.146107 pipeline.