16SrDNA  pyrosequencing  of  fecal  pellets
The bacterial DNA of  fresh mouse stool samples were extracted using the QIAmp Fast DNA Stool Mini Kit,  the V3-V4  region of  the bacterial 16S  ribosomal DNA was amplified  with  PCR and library quantification was  done  using  KAPA  Library  Quantification  Kit  for  Illumina  platforms.