GSM6606184: E14 TKO cells, Day0, batch1 replicate 11 (SMARTseq2_EpiLC); Mus musculus; RNA-Seq
Single cells were index-sorted individually by FACS (SONY SH800) into wells of a 96-well PCR plate containing 2.3 µl of lysis buffer (200U/µL SUPERase-IN (LifeTechnologies), 10% Triton X100, RNAse free H2O). scRNA-seq SMARTseq2 was performed as previously described (Picelli et al., 2014). The Illumina Nextera XT DNA kit was used to prepare libraries that were sequenced on the Illumina HiSeq 4000 (paired-end 50bp). Samples from all cell lines were included in each sequencing lane, to control for technical lane effects