GSM8426384: D0_PATZ1; Mus musculus; ChIP-Seq
ChIP experiments were performed according to standard protocols (78, 79). Lysed cells were sonicated using a Bioruptor (Diagenode) according to the manufacturer's protocol, and chromatin was immunoprecipitated with antibodies against PATZ1 (sc-390577, Santa Cruz Biotechnology) for D0 and D5, sequential PATZ1 and GTF2I (PA5-17642, Thermo Fisher) for ChIP-reChip seq, PATZ1 (A10053,Abclonal) for D-2, D0, D5, D8, and IgG (PP64, Millipore) overnight at 4°C in the presence of Protein A beads (GE Healthcare). ChIP-Seq libraries were prepared using the Kapa LTP Library Preparation Kit (Kapa Biosystems). The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina platforms, according to effective library concentration and data amount. cDNA libraries were single-end sequenced (50bp) on an Illumina HiSeq  4000.