PTS_1
Briefly, 3 and 5 adaptors were ligated to 3 and 5 end of small RNA,respectively. Then the first strand cDNA was synthesized after hybridazition withreverse transcription primer. The double-stranded cDNA library was generated throughPCR enrichment. After purification and size selection, libraries with insertions between18~40 bp were ready for sequencing on Illumina sequencing with SE50