GSM7035058: FLC tumor cell RNA-seq sample WT #2 [AF2]; Homo sapiens; RNA-Seq
Total RNA was isolated from ethanol and retinoic acid-treated FLC cells with the Total RNA Purification Kit (Norgen Biotek) per manufacturer's instructions.  RNA purity was quantified with the Nanodrop 2000 (Thermo Fisher Scientific, Waltham, MA) or Nanodrop One and RNA integrity was quantified with the Agilent 4200 Tapestation (Agilent Technologies, Santa Clara, CA). Libraries were prepared by the Cornell Transcriptional Regulation and Expression (TREx) Facility using the NEBNext Ultra II Directional RNA kit.