GSM7106606: T_81; Homo sapiens; RNA-Seq
Total RNA was harvested using NucleoSpin RNA isolatin kit (MN). 0.4 ug of total RNA was used for the construction of sequencing libraries. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumia) according to the manufacturer's instructions. After cluster generation, the libraries were sequenced on an Illumina Novaseq platform and 150 bp paired-end reads were generated.