RNA-seq of bulk induced trophoblast stem cells reprogrammed from human embryonic stem cells
RNA-seq of bulk induced trophoblast stem cells reprogrammed from human embryonic stem cells
Samples were collected from bulk cells cultured in vitro. RNA was isolated using TRI reagent (Sigma-Aldrich; Cat. No.T9424) and DNase I-treated (Ambion; Cat. No. AM2222). cDNA was synthesized using SMARTer Ultra Low Input RNA for Illumina Sequencing-HV kit (Clontech Laboratories; Cat. No. 634820) according to the manufacturerâ€™s guidelines.