GSM6807418: PC3F12K_3; Homo sapiens; RNA-Seq
RNA was extracted using PureLink RNA Mini Kit (Thermo Fisher Scientific), followed by DNase I treatment with TURBO DNA-free Kit (Invitrogen).  Quality control was performed with Agilent 2100 Bioanalyser. RNA libraries for mRNAseq were started by using NEBNext® Poly(A) mRNA Magnetic Isolation Module to enrich the polyA RNA from total RNA, then library prep with KAPA RNA HyperPrep Kits according to the manufacturer's guidelines