GSM8207656: B4galt1 WT, replicate 3; Mus musculus; RNA-Seq
Bone marrow (BM) cells were obtained by flushing mice tibia and femur shafts in 1×PBS supplemented with 3% FBS and 5 mM EDTA (flushing buffer) through a 70mm filter, followed by erythrocyte lysis 1× RBC Lysis Buffer (eBiosciences). Library preparation followed manufacture's recommendations in the SMART-Seq v4 Ultra Low Input RNA kit (Takara) Final preparation and amplification of libraries was completed with the SMARTer ThruPLEX DNA-seq Kit (Takara) utilizing dual 8bp indexes.  Final libraries were check by fragment analysis (Agilent, Bioanalyzer), quantified and pooled by qPCR (Kapa Library Quantification Kit, Kapa Biosystems).  Samples were sequenced over 2 lanes on the HiSeq2500, run in rapid mode with 2x150bp read lengths captured