GSM8155711: RNAPII_JMURTK2_Mock_CP_24h; Homo sapiens; OTHER
Cells were suspended in 1 x Wash Buffer and attached to concanavalin A beads, permeabilized with 100 μL of Antibody Binding Buffer containing digitonin. Libraries were created by Takara Bio using the ThruPLEX DNA-Seq Kit (Takara, R400674) and amplified for 16 cycles under the following conditions: denaturation at 98°C for 20 s, followed by annealing and extension at 72°C for 50 s. The samples were sequenced by Takara Bio using the NovaSeq 6000 platform (Illumina), with 150 bp paired end reads.