GSM8366306: embryoE7.75, cYAPKO, bio rep1; Mus musculus; RNA-Seq
E7.75 embryos were isolated and placed in DMEM/10% FBS on ice. Once genotypes were confirmed, embryos were pooled and dissociated using trypsin for 5 minutes at 37°C. After dissociation, trypsin was neutralized with three times the amount of DMEM/10% FBS. The 10x Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1 was used to perform single cell and library preparations. Generation of gel beads in emulsion, barcoding, GEM-RT clean-up, complementary DNA amplification, and library construction were all performed as per the manufacturer's protocol.