Nanopore cDNA-Seq of FAdV-4: LMH
RNA samples were extracted from chicken LMH cells at 12, 18 or 26 hours post FAdV-4 infection, and subjected into Illumina strand-specific RNA-seq or Nanopore full-length PCR-cDNA se-quencing. After removing the reads of host cells, the data of FAdV-4 Nanopore full-length cDNAs (transcripts) were corrected with reads from the Illumina RNA-seq, mapped to viral genome and then used to predict viral open reading frames (ORFs).