Bulk RNA of Subclone 4 of Bridge Population 1a
Total RNA was extracted from fresh frozen cell pellet samples using the RNeasy Plus Universal mini kit (Qiagen). RNA samples were quantified using a Qubit 2.0 Fluorometer (Life Technologies) and RNA integrity was evaluated using a TapeStation 4200 (Agilent Technologies). ERCC RNA Spike-In Mix kit (cat. 4456740, ThermoFisher Scientific), was added (but not used) and sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB). The quality of the sequencing libraries were validated on the Agilent TapeStation (Agilent Technologies) and the concentration of the libraries were quantified by using Qubit Fluorometer and by quantitative PCR (KAPA Biosystems). The samples were sequenced on an Illumina instrument (4000 or equivalent) with 2 x150 bp paired end configuration with an average of ~60 million reads per sample.