GSM8207517: NK_DMSO_rep1 (ATAC-seq); Homo sapiens; ATAC-seq
100,000 NK cells were washed once with ice cold PBS and lysed with cold lysis buffer (containing 10 mM Tris-HCl, 10 mM NaCl, 3 mM MgCl2 and 0.1% IGEPAL CA-630 (Sigma-I8896)). After lysis, Nuclei were harvested by centrifugation and DNA was digested with Tagment DNA Enzyme 1 (Illumina, 15027865) for 30 min at 37°C. Tagmented DNA fragments were purified using Monarch PCR and DNA Cleanup Kit (T1030L). Tagmented DNA fragments were amplified for 11 cycles by PCR using Q5 High Fidelity DNA Polymerase (NEBNext Ultra II Q5 - M0544) and Ad1 and Ad2 primers. (The quality, size and concentration of cDNA libraries were checked using the High Sensitivity NGS kit Fragment Analyzer and qPCR (ROCHE Light Cycler 480).I am not sure) PE50