GSM7034580: P6_BL6Retina_3; Mus musculus; RNA-Seq
Total RNA was prepared from dissected retinas (n=3 / library) by TRIzol (ThermoFisher) and RNeasy Micro kit (Qiagen) isolation. Quantification using Qubit RNA Broad-range assay Kit (Thermo Fischer Scientific). 200-400 ng of total RNA from each sample was used for constructing RNA-seq libraries with SMARTer total RNA Sample Prep kit (TakaraBio). Each RNA-seq librariy was constructed from ~250 ng purified RNA samples by SMARTer total RNA Sample Prep kit (TakaraBio).