GSM8067258: Lung, chronic_infected_D1; Mus musculus; OTHER
Probe Hybridization (User guide CG000407) we introduce our customized probes according with 10x Technical note. We refined the Visium protocol to reinforce the Probe Hybridization and we exploited the Diluted Permeabilization Enzyme B at 100X in Buffer EB. Similar approach was also used for the control uninfected lung. Formalin Fixed Paraffin-embedded tissues (FFPEs) were sectioned at 5 µm in thickness and attached to a Visium Spatial Gene expression slide. The tissue sections underwent hematoxylin and eosin staining using the Visium Spatial Gene Expression protocol designed for FFPE tissue - Deparaffinization, H&E Staining, Imaging & Decrosslinking demonstrated protocol. Imaging was performed using a scanning tile microscope (Aperio AT2, Leica). Then slides underwent tissue removal and library preparation following Visium Spatial Gene Expression for FFPE libraries demonstrated protocol.