GSM7743251: heart_mdx_ctrl_rep4; Mus musculus; RNA-Seq
For the DMD study, RNA was extracted in Trizol (TriPure, Roche) from frozen ventricles or GM tissue, using the NucleoSpin RNA extraction kit (Macherey-Nagel). From age mice, total RNA was extracted from GM using NucleoZOL reagent (740404.200, Macherey-Nagel). RNA libraries were prepared for sequencing using standard protocols with polyA enrichment. Paired-end RNA sequencing was performed using BGISEQ-500 for the DMD study and Illumina NovaSeq for the aging study.