GSM8156077: 3T3-L1, siCon, -MDI, rep4; Mus musculus; ATAC-seq
Cells were lysed and nuclei were collected and stored in cell lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2,0.1% IGEPAL CA-630). Nuclei were exposed to TDE1 Tagment DNA enzyme (Illumina), followed by transposition, DNA extraction, and amplification of DNA with Illumina Nextera ATAC Series (i5 and i7) primers