GSM8154815: Isolated_tumor_cell-Stress-ATAC-seq-Rep1; Mus musculus; ATAC-seq
The transposition was assessed using the Nextera DNA Library Preparation Kit (Illumina). The nuclei (5 x 104) were pelleted and resuspended with transposase for 30 min at 37 °C. The transposed DNA fragments were then purified instantly with a MinElute PCR Purification Kit (Qiagen) and PCR-amplified using 1 × NEB Next High-Fidelity PCR Master Mix (New England Biolabs). Subsequent libraries were purified with the MinElute PCR Purification Kit (Qiagen) and subjected to sequencing on Illumina Novaseq 6000 using PE150.