RNA-seq of Homo sapiens Huh7 cell line
Total RNA was extracted using the TRIzol reagent according to the manufacturers protocol. RNA  purity and quantification were evaluated using the NanoDrop 2000 spectrophotometer (Thermo  Scientific, USA). RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent  Technologies, Santa Clara, CA, USA). Then the libraries were constructed using TruSeq Stranded  mRNA LT Sample Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturers  instructions.