S60_rRNAdepletedRNA
Mycelial samples were purified with a miRNeasy Mini Kit (QIAGEN) following the procedure for total RNA purification supplied by the manufacturer. The same total RNA sample was used to generate both small and rRNA-depleted RNA libraries and subsequent sequencing, aiming to minimize biological differences between small and long RNA datasets from the same strain. Long RNA libraries were prepared by an rRNA removal method, using Illumina Stranded Total RNA Prep with Ribo-Zero Gold rRNA Removal Kit. This method allowed using customized M. lusitanicus 28S, 18S, and 5.8S rDNA probes, predicted by Barrnap v0.9. Libraries were loaded into an Illumina NovaSeq 6000 S Prime flow cell and sequenced for 150-bp paired-end reads