GSM6753478: OD_1.0_r1; Salmonella enterica subsp. enterica serovar Typhimurium; RNA-Seq
Bacterial RNA was isolated from Salmonella strain SL1344. RNA extraction was performed with 1.8 ml of each in vitro culture using the TRIzol reagent (Invitrogen) according to the manufacturer's recommendation. RNA quality was checked using Qubit RNA High Sensitivity Assay Kit (Invitrogen) and 2100 Bioanalyzer RNA 6000 Pico/ Nano kit (Agilent Technologies). Prior library preparation, DNAse treatment was performed using DNAse I kit (Thermo Fisher) followed by ribosomal RNA depletion. Ribosomal RNA (rRNA) was depleted using Lexogen's RiboCop META rRNA Depletion Kit protocol according to manufacturer's recommendation using 100ng total RNA as input per sample. DNA libraries suitable for sequencing were prepared using CORALL Total RNA-Seq Library Prep protocol (Lexogen) according to the manufacturer's recommendation with 13 PCR cycles. Library quality was checked using a 2100 Bioanalyzer DNA High Sensitivity kit.