GSM7107660: wtpromoter_Sir3-13xMyc ChIP rep 1; Saccharomyces cerevisiae; ChIP-Seq
Cells were lysed by mechanical disruption in a bead beater. Chromatin was fragmented by sonication using a BioRuptor. All immunoprecipitations were performed using Pierce Anti-c-myc magnetic beads (Thermo Fisher Scientific 88843). DNA was purified using Qiagen spin column kit NEB Ultra II library prep kit