MinION sequencing of Staphylococcus saprophyticus F5_7S_P13
Biomass was suspended in 1 mL of buffer (100 mM Tris HCl, 50 mM NaCl, pH 7.0), and genomic DNA was extracted via a lysozyme, proteinase K treatment followed by phenol-chloroform extraction and ethanol precipitation.  All gDNA manipulations were performed using wide-bore pipet tips to prevent gDNA shearing. Native genomic DNA (gDNA) was sequenced on a MinION R9.4 flowcell . The sequencing library was prepared using the ONT Rapid Barcoding Sequencing kit (SQK-RBK004) according to the manufacturers protocol.