gut microbiota and autism 49
After polymerase chain reaction (PCR) amplification and sequencing on Illumina platform, raw fastq files were processed using the Quantitative Insights into Microbial Ecology 2 (QIIME 2). For each representative sequence, the feature-classifier and algorithm in QIIME2 were employed to annotate taxonomy classification based on the information retrieved from the GreenGenes and Silva databases.