Long read direct RNA sequencing of WT saccharomyces cerevisiae
Total RNAs were treated with DNAse I (Invitrogen, catalog #: 18-068-015) following the manufacturers protocol. Using the direct RNA sequencing kit from Oxford Nanopore (ONT,catalog #: SQK-RNA002) libraries were prepared according to the manufacturer&#39;s instructions. Sequencing was performed using R9.4 flow cells on a MinION Mk1B device and sequenced for 48 hours. Basecalling was performed using Guppy Basecaller (Version 6.1.1+1f6bfa7f8). Reads were then mapped to the Saccharomyces cerevisiae genome:(S288C_reference_sequence_R64-3-1) using Minimap 2 (Version 2.17-r941).