GSM6753261: wildtype nephrectomy, sample 2, snRNAseq; Mus musculus; RNA-Seq
brains were extracted followed by isolation of nuclei using FACS. For experiments utilizing the 10x Genomics platform, the following reagents were used: Chromium Next GEM Single Cell 3′ Library kit v3.1 (PN-1000158), Chromium Next GEM Single Cell 3′ GEM Kit v3.1 (PN-1000130), Chromium Next GEM Single Cell 3' Gel Bead Kit v3.1 (PN-1000129) and Dynabeads MyOne SILANE (PN-2000048) and were used according to the manufacturer's instructions in the Chromium Single Cell 3′ Reagents Kits V3.1 User Guide. Single nuclei droplets were generated using 10X Genomics Droplet generator and in total 10,000 nuclei per sample were targeted. Sequencing and read mapping: Generated libraries were sequenced on an Illumina NextSeq 500 at the Next Generation Sequencing Facility[ZS1]  of the Max Planck Institute of Biochemistry  with >50 x 103 reads per nucleus followed by de-multiplexing and mapping to the mouse genome (build mm10) using CellRanger v3.0 (10x Genomics) with standard settings and inclusion of introns for nuclear transcripts. Count matrixes were further analyzed with Seurat V4.2.0 R package