GSM8015113: Bisulfite RNA-seq of non-modified in-vitro transcripts (IVTs) m1, m2, m3, m4; synthetic construct; OTHER
We performed RNA bisulfite treatment (bsRNA-seq) following the protocol from Johnson et al (Johnson, Z., Xu, X., Pacholec, C. & Xie, H. Systematic evaluation of parameters in RNA bisulfite sequencing data generation and analysis. NAR genomics Bioinforma. 4, lqac045 (2022))There were nodeviations done for the protocol except the fact that a GE50 spin column was used for the removal of the excess of bisulfite reagent (sodium bisulfide and hydroquinone) instead of a GE25. This protocol was applied to RNA fully modified with m5C or non-modified obtained from in-vitro transcripts (IVTs) m1, m2, m3, m4  built from four non-overlapping fragments from the mouse canonical pre-rRNA (~13kb long)