GSM7689090: PN96_Nephron_NEAT1ko-clone1_day13_rep2; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol Reagent (Magen) and reverse transcribed into cDNA using the HiScript II 1st Strand cDNA Synthesis Kit (Vazyme) following the manufacturer's instruction. RNA-Seq libraries were prepared using VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina (Vazyme) following manufacturer's instructions. Briefly, total RNA was purified twice using poly-T oligo-attached magnetic beads before the synthesis of double-stranded (ds) cDNA. The ds-cDNA was ligated to adaptors (Vazyme) and sequenced by Illumina (Novogene).