Raw reads of saccahromyces cerevisiae ZHigh
The DNA samples that passed the electrophoresis test were randomly broken into fragments of approximately 350 bp in length using a Covaris ultrasonic breaker. The processed D NA fragments were then subjected to end repair, A-tail addition, sequencing junction addition, purification and PCR amplification using the NEBNextUltra DNA Library Prep Kit for Illumina (NEB, USA). After the library was constructed, Qubit 2.0 was used for preliminary quantification, and the library was diluted to 2 ng/ul. Subsequently, Agilent 2100 was used to detect the insert fragments of the library, and after the insert size met the expectation, Q-PCR was used to accurately quantify the effective concentration of the library in order to ensure the quality of the library.