GSM8156127: K700E,AAVS1-KO,non-differentiated,rep2; Homo sapiens; RNA-Seq
CD34+ cells from cord blood were CRISPR/Cas9-edited to introduce an endogenous SF3B1 K700E mutation (SF3B1 K700E/+) or have no mutation (SF3B1 +/+; AAVS1 edited as a negative control)and expanded in culture.  2 days after editing, BFP+ CD34+ cells were sorted into Trizol and RNA isolated. The remainder of CD34+ cells were differentiated into erythroid cells for 7 days. BFP+ CD71+ CD235a+ erythroid cells were sorted into Trizol and RNA isolated. Poly(A)-selected, strand-specific (dUTP method) Illumina libraries were prepared using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio) (input of 10 ng RNA was used for samples with >10 ng available; for samples with <10 ng available, entire amount was used for library prep) l and sequenced on the Illumina HiSeq 2000 to obtain∼60-80M paired-end 151 bp reads per sample.