18S rRNA of environmental samples Lake Lunz (Austria)
18S rRNA sequences obtained from amplicon sequencing analysis of the experimental community collected in 2020. The sample was first amplified using 18S rRNA primers targeting the V4 hypervariable region (TAReuk454FWD1, 5- CCA GCA SCY GCG GTA ATT CC-3, Stoeck et al., 2010; V4r, 5-ACT TTC GTT CTT GAT YRR-3, Bradley et al., 2016). Subsequently, the sample was sequenced at Eurofins Genomics Germany GmbH (Ebersberg, Germany) using Illumina MiSeq sequencing with v3 chemistry and 2x300 paired-end reads. Sequences were matched to their taxonomy using the PR2 database (Guillou et al., 2013).