GSM7067923: untargetd ZFP-ETRs treated Hepa 1-6 Pcsk9tdTomato cells, replicate 1; Mus musculus; Bisulfite-Seq
Genomic DNA extraction by Maxwell® 16 Cell LEV DNA Purification Kit and following provider indications DNA was sheared using Covaris LE220 Focused-ultrasonicator for an average 350 bp size NEBNext DNA Ultra II Reagents (NEB, Ipswich, MA) were used according to the manufacturer's instructions for end repair, A-tailing and adaptor ligation of EM-seq adaptor. The ligated samples were cleaned up according to the manufacturer's instructions with NEBNext Sample Purification Beads