Nanopore amplicon sequencing (HTT gene) of CAGinSTEM platform
Genomic DNA was extracted from recombinant hES H9 cell lines, and the recombinant allele at the HTT locus was PCR amplified. PCR amplicons were size selected, and a sequencing library for Nanopore sequencing was prepared using SQK-LSK110, EXP-NBD104 and EXP-NBD114 kits, following the manufacturers instructions, and loaded on a R9.4.1 MinION flow-cell. Sequencing was then carried out for 72 h and base-calling was performed with Guppy v5.0.7 sup.