RNA-seq of human: gut mucosal biopsy ulcerative colitis 23
RNA was purified with the Allprep DNA/RNA Mini Kit from Qiagen, and using the QIAcube instrument from Qiagen. TruSeq Stranded Total RNA LT Sample Prep Kit from Illumina was used to prepare whole transcriptome libraries which were then paired-end sequenced with Illumina NextSeq550. Human sequences were then removed by using bowtie2 twice.