GONU-DEHP
1micro gram of total RNA was depleted of bacterial rRNA NEBNext rRNA depletion kit (bacteria, Cat. no. E7850X) followed library preparation using NEBNext Ultra II Directional RNA Library prep kit for Illumina (Cat. no. E7760L). Further t double-stranded cDNA libraries were constructed followed by adapter ligation. Sequencing was performed for 2X150 cycles