Shotgun metagenomic sequencing of Burkholderia gladioli isolated from a canine
Illumina DNA Library Prep Kit (Illumina, San Diego, CA) with up to 500 ng DNA input following the manufacturers protocol using unique dual-index 10 bp barcodes with Nextera adapters (Illumina, San Diego, CA). All libraries were pooled in equal abundance. The final pool was quantified using qPCR and TapeStation (Agilent Technologies, Santa Clara, CA).