GSM7035052: nChIP Pro-MMP2-3XHA HA Rep2; Homo sapiens; ChIP-Seq
Mononucleosomes were liberated from nuclei using MNase. S1 and S2 fractions were pooled for ChIPs using the respective antibodies. ChIP-seq liibraries were made using the NEBNext Ultra II kit from NEB, along with their multiplex primers. The manufactureres protocol followed and number of cycles determined by spike-in of SYBR for quantification