GSM7993495: Ccr2 Cre tdTomato Day 13; Mus musculus; RNA-Seq
Hearts were collected, perfused, and enzymatically digested to a single cell supsension. Monocytes and macrophages were sorted via FACS. In the case of the Cx3cr1:CreERT2,Rosa26:tdTomato mice, additional gate was used to separate tdtomato positive and negative cells into separate libraries. Chromium Single Cell 5ʹ Reagent v1.1 kit from 10X Genomics