V3-V4 16S-Seq of aquatic bacteria: lake water
Samples were taken from the surface water of lake. The DNA from collected microorganisms was isolated using the GeneMATRIX DNA Purification Kit (EurX). PCR amplification was carried out using Q5 Hot Start High-Fidelity 2X Master Mix with region-specific (341F and 785R) primers. Sequencing took place on an Illumina MiSeq sequencer using the MiSeq Reagent Kit