Illumina NovaSeq 6000 paired end sequencing; 9 RNA-seq of mouse skeletal muscle samples
9 RNA-seq of mouse skeletal muscle samples
They are male adult ICR mice (wild type) and kept under temperature 22°C, 12 h light/dark cycle with free access to standard rodent chow and water. Sham：Muscle samples were from sham group. Sham group in which PBS was injected into the tibialis anterior muscle for 14 days；Den14d：Muscle samples were from Den group. Den group in which PBS was injected into the tibialis anterior muscle after sciatic nerve injury for 14 days；EVs14：Muscle samples were from SKP-SC-EVs group. SKP-SC-EVs group in which PBS containing SKP-SC-EVs was injected into the tibialis anterior muscle after sciatic nerve injury for 14 days. 1. sciatic nerve transection protocol - The mouse skin was sterilized and dissected in the surgical area of the left thigh, the muscle was bluntly separated to expose the sciatic nerve, and then the sciatic nerve was transected 10 mm. 2. SKP-SC-EVs treatment protocol - extracellular vesicles derived from skin-derived precursors-derived Schwann cells. mouse grow in different time mouse sample from different time Total RNA was extracted using Trizol LS. Cells were fractionated and cytoplasmic RNA was extracted using the Paris Kit (Life technologies) according to the manufacturer's instructions mRNA-Seq libraries (from polyA+ cytoplasmic RNA) were created according to the standard Illumina protocol.