GSM7884515: nos-white-KD.1 [total RNA]; Drosophila melanogaster; ssRNA-seq
Total RNA was extracted from 10 to 20 ovaries from 3-6 day-old flies using TRizol (Thermo Fisher Scientific). rRNA depletion was performed from 1µg total RNA using the RNA Depletion stranded Library Prep kit (BGI). RNA-seq libraries were produced using NEBNext Ultra Directional Library Prep Kit for Illumina, following the manufacturer's instructions for rRNA depleted RNA.