GSM7068348: Urine Epithelial Colony 3; Homo sapiens; RNA-Seq
Total RNA samples were selected for polyA+ transcripts using the Qiagen Oligotex midi kit. Resulting samples were depleted of ribosomal sequences using two rounds of RiboZero Gold™, Illumina Inc. mRNAs were fragmented by sonication, adaptors were ligated onto ends for paired-end multiplex sequencing, first and second strand DNA was synthesized.