En 8WV12WAV3W H3K27me3 ChIP-seq INPUT Replicate 2
The immunoprecipitated DNAs were incubate with mixed protein A and G Dynabeads (Thermo, Cat No./ID: 10002D and 10004D). ChIP DNAs were reverse crosslinked and purified with PCR purification kit (Qiagen, Cat No./ID: 28206). After quantification using Qubit 4 Fluorometer (Invitrogen), 12 ng of input or 0.5 ng of IP DNA was used for construction of ChIP-seq library. Template preparation, library synthesis, library amplification and purification were performed according to the manual of SMARTer ThruPLEX DNA-Seq kit (Clontech, Cat No./ID: R400676).