Shotgun metagenome sequencing of Bos taurus skin microbiome: skin scabs developed during lumpy skin disease
Metagenomic DNA was extracted with QIAamp DNA Microbiome Kit (Qiagen, Germany), according to manufacturers instructions.400 nanograms of DNA from each sample was used as an input for library prep. DNA was fragmented to 250  320 bp fragments with Covaris ME220 Focused-ultrasonicator. Standard fragment DNBSeq-compatible libraries were prepared using MGIEasy Universal DNA Library Prep kit and further circularized with MGIEasy Circularization Kit (both  (MGITech, Peoples Republic of China), according to the instructions provided by the manufacturer