GSM6419933: β1 transduced+CLI 1; Homo sapiens; RNA-Seq
RNA was extracted using Trizol protocol, followed by DNase treatment.  RNA was then re-purified with an aqueous column-based protocol followed by gel analysis to analyze purity and quality of the RNA. The RNAseq libraries were prepared with Illumina's 'TruSeq Stranded mRNAseq Sample Prep kit' (Illumina). Read 1 aligns to the ANTISENSE strand. The libraries were pooled; quantitated by qPCR and sequenced on one S2 lane for 101 cycles from one end of the fragments on a NovaSeq 6000.