12558_H3K27ac_CHIPSeq
We performed 2X50nt paired-end sequencing at the Princeton University Lewis-Siglers Genomics core facility. All reads were aligned to the CanFam 3.1 reference genome using bowtie2 v2.4.5 and sorted using samtools v1.9. Since libraries were PCR amplified, we removed duplicates (strands with same unique molecular identifiers) using umi_tools v1.1.2.