GSM7824325: NLRP3(STZ) rep2; Mus musculus; RNA-Seq
Placenta were homogenised. CD45+ cells were sorted by flow cytometry and collected for RNA isolation. The cDNA synthesis and amplification were done as recommended by Clontech. cDNA libraries were prepared using Nextera XT DNA Sample preparation reagents. Fragmented and tagged libraries were quantified using an agilent bio-analyzer, pooled and sequenced on a HiSeq 6000 platform (Illumina) using the pair-end 75-bp sequencing strategy.