GSM8067245: Colon IBD; Homo sapiens; RNA-Seq
Single cells suspensions generated from colon biopsies from a non-IBD healthy control and a pan-colitic IBD patient were used for scRNA-seq. Tissue samples were thawed into warm media with fetal bovine serum (FBS) and preservation media was removed by washing. Samples were treated with EDTA pre-digestion with rotation for to remove dead/damaged epithelial cells. Samples were then dissociated enzymatically into a single-cell suspension with rotation. Cells were washed, strained and counted for viability using AO/PI and 10,000 cells were loaded onto 10X channels The GEX 3' V3 protocol was followed throughout sequencing. Libraries were sequenced on an Illumina NovaSeq instrument.