amplicon sequencing of X-ComEC PCR product from no-template control sample
Library preparation was performed using the Rapid sequencing gDNA-barcoding kit with purified PCR product as input. Sequencing was performed on the minION flowcell R9.4.1. Basecalling with guppy basecaller with high accuracy configuration file (dna_r9.4.1_450bps_hac.cfg) with minimum quality score filter >7 and demultiplex with guppy barcoder.