GSM6415982: 1016 H1; Homo sapiens; RNA-Seq
liver organoids were disociated into single cells using trypsin. Single cell suspension was  resuspended in the master mix and loaded together with partitioning oil and gel beads into the chip to generate the gel bead-in-emulsion (GEM). scRNA-seq libraries were prepared using the Chromium Single Cell 3′ Reagent Kit (10x Genomics). Samples were sequenced using a HiSeq 2500 system.