GSM7884684: KO6; Mus musculus; RNA-Seq
Total RNAs were extracted by using the E.Z.N.A.® Total RNA Kit (Omega Bio-tek) according to the manufacturer's protocol.Total RNAs before elution were treated with TURBOTMDNase (ThermoFisher Scientific, AM2238). RNA integrity was verified on a Bioanalyser 2100 with RNA 6000 Nano chips (Agilent technologies). RNA integrity number score was above 9 for every sample. Libraries were prepared using Truseq® stranded mRNA Sample Preparation Kits (Illumina) following manufacturer's instructions. Libraries were validated using QIAxcel Advanced System and quantified by qPCR using the KAPA library quantification kit.