Metagenomic Sequencing of Stool Microbiomes in Humans
Genomic DNA (gDNA) was isolated from stool using samples using the QIAmp® PowerFecal® DNA kit (QIAGEN Inc., Germantown, MD, USA). DNA quality and concentration were on a Qubit fluorometer. Purified gDNA underwent library preparation (Nextera XT, Illumina) to be paired-end (2x150 bp) sequenced using the NovaSeq v1.5 SP (Illumina, San Diego, CA, USA) targeting a sequencing depth of 40M sequences per sample.