GSM7823634: CTCF_ChIP_PS_WT_rep1; Mus musculus; ChIP-Seq
Germ cells were isolated from mouse testis by STA-PUT method or MACS. Cells were fixed in 1% formaldehyde for 10 min at room temperature. Nuclei were isolated by lysis buffer. Chromatin was sonicated by Covaris sonicater and used for ChIP experiments. DNA libraries were prepared through the ChIPmentation method (PMID: 26280331).