The complete genome of Tissierella sp. Yu-01 isolated from the feces of black soldier fly
The gDNA was separated into two parts. One was randomly fragmented to build a library with an insertion of 300bp. The library was sending on illumina NovaSeq 6000 platform performing a pair-end 150bp sequencing strategy. Another was straightly through end-repair, 3 adenylated, adapters and Motor protein ligations. The product was purified using Agencourt AMPure XP Beads(Beckman, A63881). Finally, fragments larger than 1KB were screened for Single-molecule nanopore DNA sequencing on MinION Flow Cell (ONT, R9.4.1).