GSM7916134: Low-grade glioma, Combination_4, Day37; Mus musculus; RNA-Seq
The tumor samples were collected from IDH-mutant mice after 9 days treatments and cut into 1 mm3 with surgical blade. Samples were sent on ice to Benagen Technology Co., Ltd for immediate downstream RNA sequencing. In brief, following RNA isolation with Trizol (catalog #, check with Benagen), a total amount of 2 μg RNA per sample was used as input material for library preparations. Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (#E7530L, NEB, USA) following the manufacturer's recommendations and index codes were added to attribute sequences.