ONT-DNA-seq:Chlamydomonas reinhardtii CLIP-mutant: LMJ.RY402.143080 (prr9)
Genomic DNA isolation of C. reinhardtii mutants was accomplished using the Quick-DNA Miniprep kit (Zymo Research) according to manufacturer instructions. For nanopore long read sequencing, the ligation sequencing kit with native barcodes (SQK-LSK114 with SQK-NBD114.24) was used according to the manufacturers protocol (Oxford Nanopore Technology). Long read data were produced on a R10.4.1 flowcell (Oxford Nanopore) which was run on a GridION X5 sequencing platform and basecalled using guppy47 v7.0.9 in super-accuracy mode.