raw sequencing data of melospiza melodia sanaka
DNA extracted using Dneasy blood and tissue kit (Qiagen, CA, USA), and concentrations quantified using Qubit BR dsDNA assay kit (Life Technologies). 150ng of DNA from each sample was used to prepare individually barcoded libraries with 300bp insert size following NEBNext Ultra II FS DNA library prep kit protocol (Illumina, CA, USA).