scRNAseq of human NK: Gene Expression library
cDNA was prepared after 10x Genomics GEM generation and barcoding, followed by the GEM-RT reaction and bead cleanup steps.  Purified cDNA was amplified before being cleaned up using SPRIselect beads. The supernatant was then collected for the Cell Surface Protein Library.  Samples were then run on a Bioanalyzer to determine the cDNA concentration for the GEX library preparation. GEX (Gene Expression) and TotalSeqC Feature libraries were prepared as recommended by 10x Genomics for Feature Barcode technology for Cell Surface Protein(v1/v2 Chemistry) user guides with appropriate modifications to the PCR cycles based on the calculated cDNA concentration. The concentration of each library was accurately determined through qPCR utilizing the KAPA library Quantification Kit according to the manufacturer's protocol (KAPA Biosystems/Roche) to produce cluster counts appropriate for the Illumina NovaSeq6000 instrument.