GSM7774385: C30S70_Rep1-3; Homo sapiens; RNA-Seq
30um of tissue sections were lysed in TRIzol reagent (Thermo Fisher Science) and extract RNA by using Direct-zol Kit (Zymo Research). Libraries were prepared by BRB-Seq(Alpern D, et al.) method. Individual samples were reverse transcribed using dT primer with unique cell barcodes. Then replicate were mixed and amplified by PCR. 10nt of UMI followedby 9nt of cell barcode are in read1 and 81nt of RNA insert reads are in read2.