Hi-C of Tiarella polyphylla
Formaldehyde was used to fix the sample cells so that DNA and protein, protein and protein were cross-linked. After the sample cross-linking was completed, the cells were lysed, and the sample quality was tested by sampling and extraction. After qualified detection, it enters the preparation process of "HI-C fragment". Chromatin digestion was carried out by restriction enzyme, and the digestion effect was detected by sampling. After biotin labeling, flat end ligation and DNA purification extraction, HI-C samples were prepared and sampled for DNA quality testing. After qualified detection, the library was entered into the standard library construction process. The HI-C fragment was determinated by biotin, interrupted by ultrasound, and the end was repaired, base A was added, and sequencing adaptor was added to form the adaptor product. Then the PCR conditions were screened and amplified to obtain the library products. The amplified products of the library were sampled for "HI-C fragment junction quality control detection", and the whole library preparation was completed after the detection was qualified.