Microbiome and inflammatory biomarkers related to cardiovascular disease in transgender women with HIV
Sequencing of the V3-V4 region of the bacterial 16S rRNA gene was performed using the 515FB-806RB primer set,dual-indexing qPCR was used to barcode and add indexes for gene amplicon sequencing. The resulting amplicons were normalized, pooled, cleaned, quantified, and then sequenced with a 2x300 bp cycle with 15% PhiX. Methods protocol published in https://doi.org/10.1038/nbt.3601 paper.