RNA-Seq of Mesocricetus auratus: brain
Analyses were preceded by a quality check of RNA in the samples using Agilent RNA 6000 Nano kit, considering as acceptable RIN values >= 8. Libraries were prepared from 500-1000 ng of total RNA with the Truseq Stranded mRNA library preparation kit (Illumina), following manufacturers instructions and were run on an Agilent 2100 Bioanalyzer using an Agilent High Sensitivity DNA kit (Agilent Technologies) to ensure the proper range of cDNA length distribution. Sequencing was performed on Illumina NextSeq with NextSeq 500/550 High Output Kit v2.5 (300 cycles; Illumina) in pair-end mode.