Ion Torrent PGM sequencing of SSUB029899 : ITS amplicon analysis of tongue mycobiota ISEA239
The ITS2 region was amplified using the following primers with the sample-specific 8-base tag sequence: 5.8SR (TCG ATG AAG AAC GCA GC) and ITS4 (TCC TCC GCT TAT TGA TAT GC). PCR amplification was carried out using KOD One polymerase. Reads were excluded from the analysis if they were shorter than 200 bases or did not include the correct forward and reverse primer sequences.