GSM8015345: placenta_16; Homo sapiens; RNA-Seq
DNA, RNA, and miRNA were simultaneously extracted from placenta, powdered under liquid N2, using the AllPrep miRNA kit (Qiagen) and the QIAcube for automated nucleic acid extraction (Qiagen, USA). Total RNA was sent to MedGenome Inc. (Foster City, CA, USA) for RNA QC, library preparation, and sequencing. cDNA libraries were generated using the Illumina Stranded Total RNA kit (Illumina, CA, USA) and sequenced using 100bp paired-end reads on the HiSeq 2500 platform (Illumina, CA, USA).