Evidence for immune activation in pathogenesis of the HLA class II associated disease, podoconiosis
Evidence for immune activation in pathogenesis of the HLA class II associated disease, podoconiosis
Whole blood was collected from podoconiosis patients and healthy controls using PAXgene tubes. RNA was extracted from whole blood samples collected in PAXgene tubes using MagMAXTM bead-based extraction method Libraries were prepared using the QIAseq Stranded RNA library kit (Qiagen #180450) and with globin and rRNA removed using the QIAseq FastSelect rRNA and globin mRNA removal kit (Qiagen #335376)