GSM7774222: E0771, Malt1_WT, Rep2; Mus musculus; RNA-Seq
Total RNA was isolated from cells using RNAiso Plus Regent (Takara, Cat# 9108) following the manufacturer's instructions. The NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, Cat# E7765) was used to generate sequencing libraires from 1 μg of total RNA. All library preparations were conducted according to the manufacturer's instructions. Paired-end 150bp sequencing was performed using an Illumina NovaSeq platform.