GSM8068283: Overexpression con1; Homo sapiens; RNA-Seq
Transfected SiHa cells were transferred into a nuclease-free microcentrifuge tube (MCT), washed with 1x PBS, and the addition of 1.0mL TRIzol® (Sigma). Three biological replicates for each group (for both OE and KD) were maintained. RNA extraction was done by using instructions of the manufacturer and DNaseI (Roche) treatment was given followed by the purity and concentration check by NanoDrop® 2000 (Thermo Scientific, Inc) and also checked on the 1% agarose gel. Purified RNA was used for RNA sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols