long-read bovine IgM repertoire: heifer 3
Whole blood was collected and peripheral blood mononuclear cells were isolated. IgM+ B cells were sorted using magnetic bead sorting. The B cells were harvested, and RNA was extracted using TRIzol. cDNA was produced and used for PCR to amplify IgM transcripts. End prep, barcoding, and adapter ligation were completed, and samples were sequenced using the ONT MinION.