RNA-seq of mouse colon: CON5
Total RNA and DNA was isolated using Qiagen AllPrep DNA/RNA kits. One microgram of total RNA was shipped to The Center for Applied Genomics (Hospital for Sick Children, Toronto, ON) for quality control, library preparation, and sequencing. RNA quality was confirmed with a Bioanalyzer and libraries were prepared using a NEB Ultra II Directional mRNA library kit. Samples were then processed using random primers and paired end sequencing using an Illumina HiSeq 2500 yielded 50-90 million reads/sample.