GSM7156535: male normal control mice, male control 2, 14 weeks; Mus musculus; RNA-Seq
Total RNA from these kidney samples was separately isolated with Trizol reagent (Invitrogen, Carlsbad, CA, USA) and purified using the RNeasy Animal Mini Kit (Qiagen, Valencia, CA). RNA quality was then assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies, PaloAlto, Calif) RNA libraries for RNA-seq were prepared using BGISEQ-500RS High-throughput Sequenciing Set Kit following manufacturer's protocols.