GSM8155092: RNASEQ, clone SC9, NELFE, replicate 3; Homo sapiens; RNA-Seq
One million cells were harvested, washed once in cold PBS, resuspended in 600 µL of RTL buffer (RNeasy Mini kit, 74104, Qiagen) and stored at -80°C until subsequent RNA isolation. RNA was isolated using the RNeasy Mini kit (74104, Qiagen). Libraries were prepared using the TruSeq® RNA LT kit and TruSeq RNA Single Indexes (Illumina). Libraries were sequenced with single-end 65-bp reads on a HiSeq 2500 platform