GSM7152990: shH3f3b_H3.3_2; Mus musculus; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a E220 focused-ultrasonicator (Covaris). DNA libraries for ChIP-seq were prepared using Nextera XT DNA Library Preparation Kit following manufacturer's protocols.