GSM7070810: bone marrow-derived macrophages, Znrf1 knockout#1, untreated; Mus musculus; RNA-Seq
Total RNAs were prepared from BMDMs and further purified by the TruSeq Stranded mRNA Library Prep Kit following the manufacturer's recommendations. Briefly, one mg total RNAs was subjected to oligo(dT) magnetic beads, fragmented and reverse transcribed to synthesize the first strand cDNA using random primers. After the generation of double-strand cDNA and adenylation on the 3' ends of the DNA fragments, the adaptors were ligated and purified with the AMPure XP system. The quality of the libraries was assessed on the Agilent Bioanalyzer 2100 system and a Real-Time PCR system.