GSM6856833: Bovine assembled blastoids day 4; Bos taurus; RNA-Seq
Bovine blastoids were single cell dissociated and strained cells were prepared as stated adobe. Cells were washed in PBS containing 0.04% BSA and centrifuged at 90° x500g for 5 min. Cell were resuspended in PBS containing 0.04% BSA at a single cell suspension of 1,000 cells/µL. Cells were loaded into a 10x Genomics Chromium Chip following manufacturer instruction (10x Genomics, Pleasanton, CA, Chromium Next GEM Single Cell 3ʹ GEM, Library & Gel Bead Kit v3.1) and sequenced by Illumina NextSeq 500/550 sequencing systems (Illumina).