16S rRNA of TMCRWS activated sludge
16S rRNA genes were sequenced using Oxford MinION (Oxford Nanopore Technologies, Oxford, UK). The 16S rRNA genes were amplified using the primers 27F 5-AGAGTTTGATCCTGGCTCAG-3 and 1492R 5-GGTTACCTTGTTACGACTT-3 and the amplicons were cleaned up using the DNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA) and barcoded using a 16S Barcoding Kit (Oxford Nanopore Technologies, Oxford, UK) following the manufacturers protocol. barcoded PCR products were pooled and cleaned up using Mag-Bind TotalPure NGS beads (Omega Bio-Tek, Norcross, GA) and sequenced using the Oxford Nanopore Technologies  MinION (Oxford, UK)