GSM6753957: LCS7711_QNZ_treated_921_3_Clean_Data [T-anti-FGL2-3]; Mus musculus; RNA-Seq
After 72 h coculture of T cells with DBT cells at a ratio of 4:1, T cells were collected and stained with anti-CD4 and anti-CD8 antibodies for 30 minutes at RT, then live CD8+ T cells were sorted with a FACSAria III flow cytometer (BD Biosciences). After spindown, cell pellets were frozen and shipped on dry ice to LC Sciences for total RNA extraction and total RNA NGS services. RNA libraries were prepared for sequencing using standard Illumina protocols