16S Amplicon Sequencing of Swine Fecal Microbial Communities
Illumina MiSeq paired-end sequencing with 250 bp read length using universal 16S rRNA gene bacterial primers [515F (5-GTGYCAGCMGCCGCGGTAA-3; 26) and 806R (5-GGACTACNVGGGTWTCTAAT-3; 27)] amplifying the variable region V4.