GSM8253779: ChIP-seq, H3K27me3, 2205 in vitro, AG120, Replicate 1; Mus musculus; ChIP-Seq
20 million cells per replicate for each condition (DMSO, AG120, IFNγ, AG120 + IFNγ) were cross-linked using 1% formaldehyde for 10 minutes at room temperature and quenched using glycine for 5 minutes. ChIP experiments were performed using SimpleChIP Enzymatic Chromatin IP Kit based on manufacturer's protocol. Sequencing libraries were prepared using the NEBNext Ultra II DNA library preparation kit (New England Biolabs)