GSM7823568: U251, 1Gy I, 6h; Homo sapiens; RNA-Seq
Total RNA was isolated from cells using an RNA purification kit (GeneAll) as directed by the manufacturer. Messenger RNA (mRNA) was enriched from 1 μg of total RNA by Poly(A) mRNA Magnetic Isolation Module (New England Biolabs), following the manufacturer's protocol. The NEBNext Ultra RNA Library Prep Kit (New England Biolabs) was used to create cDNA libraries in accordance with the manufacturer's instructions.