16s rRNA seq of mouse: STING KO DSS and ITE treatment 4
The amplification of the V1-V9 region of the 16S rRNA gene was conducted by using the barcoded outer primer mixture (3%) from the PCR Barcoding Kit. The amplified DNA was purified using AMPure XP and quantified by a NanoDrop 1000. The prepared DNA library was mixed with of Sequencing Buffer and sequenced on the MinION Mk1B.