Glycine max (UC3, PI 547438) black seed coat mutation genome assembly
Library preparation and long read sequencing were  conducted by the University of Illinois Sequencing Center. The HMW DNA was sheared with a Megaruptor 3 (Diagenode.com) to an average fragment length of 13 kb. Sheared DNAs were run on the BluePippin (Sage Science) using a 0.75% agarose cartridge for size selection of fragments > 10kb in length.  Size selected DNAs were converted to a library with the SMRTBell Express Template Prep kit 2.0. Each library was sequenced on 2 SMRTcells on a PacBio Sequel IIe using the CCS sequencing mode and a 30h movie time.