GSM7611225: sk-T1, senescence escaped SKBR3 ,re1; Homo sapiens; RNA-Seq
Total RNA was isolated and purified using TRIzol reagent(Invitrogen, Carlsbad, CA, USA) following the manufacturer's procedure. The average insert size for the final cDNA library was 300±50 bp. At last, we performed the 2×150bp paired-end sequencing (PE150) on an Illumina Novaseq™ 6000 (LC-Bio Technology CO., Ltd., Hangzhou, China) following the vendor's recommended protocol.