Illumina NovaSeq 6000 paired end sequencing; RNA-seq of human NSCL cell lines with induced fusion genes; ALK-EML4-V1, ALK-EML4-V3, ALK-TFG, ALK-KIF5B
RNA-seq of human NSCL cell lines with induced fusion genes; ALK-EML4-V1, ALK-EML4-V3, ALK-TFG, ALK-KIF5B
DNAse treated total RNA samples dissolved in nuclease free water NL20 cells were transfected with ALK-fusion constructs (pLVX-TetOne-EML4-ALK-V1, pLVX-TetOne-EML4-ALK-V3, pLVX-TetOne-KIF5B-ALK and pLVX-TetOne-TFG-ALK) or pLVX-TetOne (empty vector) respectively using X-tremeGENE HP DNA transfection reagents according to the manufacturers instruction. After transfection, cells were selected with 1g/ml puromycin (ThermoFisher) to generate stable cell lines. Promega Total RNA Isolation Kit Eukaryotic Transcriptome Library