GSM7108140: BeeSTINGseq_HTO-B; Homo sapiens; OTHER
For STINGseq-v1, we ran one lane of a 10x Genomics 5' kit (Chromium Single Cell Immune Profiling Solution v1.0, 1000014, 1000020, 1000151) with cell hashing performed using four hashtag-derived oligonucleotides (HTOs). For STINGseq-v2, we ran four lanes of a 10x Genomics 5' kit (Chromium Next GEM Single Cell 5' Kit v2 1000265) with cell hashing performed using eight HTOs. For BeeSTINGseq, we ran three lanes of a 10x Genomics 5' v2 kit (Chromium Next GEM Single Cell 5' Kit v2 1000265) with cell hashing performed using nine HTOs. Gene expression (cDNA), hashtags (HTOs), guide RNA (Guide-derived oligos, GDOs) libraries, and oligo-tagged antibodies (ADTs) were constructed by following 10x Genomics and ECCITE-seq protocols. We sequenced all cDNA, HTO, GDO, and ADT libraries with  a NextSeq 500 or NovaSeq 6000 (Illumina). ECCITE-seq sequencing strategy, with the following read recipe: R1 = 26; i7 = 8; R2 = 57