16S rRNA amplicons of Fayetteville Green Lake Particle Interceptor Trap collections
preserve with RNALater on Sterivex filter; RNALater removal/rinsing from Sterivex filter; freeze 1/2 treated filter in lysis buffer; proteinase K and lysozyme incubations; 25:24:1 phenol:chloroform:iso extraction, 24:1 chloroform:iso extraction, 1000 kDa ultracentrifugation; purify with Zymo kit;