WGS of Nicotiana attenuata: genotype P_331
The seeds of Nicotiana attenuata plants used for sequencing was collected by Ian T. Baldwin from different parts of Great Basin Desert, USA. Plants were grown with a day/night cycle of 16h (26-28 C)/ 8h (22-24 C) in glasshouse and HMW gDNA was isolated from 300mg of frozen leaf tissue at Max Planck Institute for Chemical Ecology, Jena, Germany. The sequencing was then perfomed at BGI TECH SOLUTIONS (HONGKONG) CO on Hiseq 3000.