ITS-AmpliSeq of wheat rhizoplane soil: ST2E2
Soil DNA was extracted from 0.25 g soil using the DNeasy PowerSoil Pro kit (Qiagen, Venlo, Netherlands) following the manufacturers instructions. Fungal ITS region was amplified using dual index paired-end sequencing using the Illumina MiSeq platform with the primers ITS2 (5- GCTGCGTTCTTCATCGATGC-3) and ITS5 (5- GGAAGTAAAAGTCGTAACAAGG-3) targeting the ITS 1 region, performed at Novogene (UK) Company Limited (Milton, Cambridge, UK). Raw sequence reads were demultiplexed, merged, and quality filtered at Novogene, producing the following fastq file.