clone 11 APOBEC1+RBM47 +doxycycline
The RNA was extracted using the Directzol RNA miniprep kit Zymo Research), and RNA integrity was assessed by utilizing the Agilent 2100 Bioanalyzer RNA Nano assay (Agilent Technologies, Santa Clara, CA, USA). Following quality and integrity verification, 1 g of total RNA was used for library preparation with the TruSeq Stranded Total RNA-Zero Gold (Illumina, San Diego, CA, USA). The final step involved sequencing the libraries on an Illumina NextSeq 550 with the Nextseq550 high-throughput kit (v2).