GSM8014677: mouse-cortex-WT-RIP-Ybx1-rep2; Mus musculus; RIP-Seq
To identify the target mRNAs of the m5C reader protein Ybx1, we carired out anti Ybx1 RNA Immunoprecipitation (RIP) followed by RNA-sequencencing. Using EZ-Magna RIP  RNA-Binding Protein Immunoprecipitation Kit (Millipore), RNA from wild type mouse cortex was pulled down by rabbit polyclonal anti-Ybx1 (abcam). The library was constructed by Novogene Corporation. Subsequently, pair-end sequencing of sample was performed on Illumina platform (Illumina, CA, USA). Library quality was assessed on the Agilent Bioanalyzer 2100 system.