GSM7337196: Smartseq2, MI, MAD2L1BP (R285*), rep1; Homo sapiens; RNA-Seq
MI oocytes were washed at least five times in 1XPBS, and directly lysed in Lysis Buffer MasterMix (0.3% TritonX-100, 40U/µL RNase inhibitor, 2.5µM oligodT30VN, and 2.5µM dNTP mix) Library was performed according to the Smart-seq2 method (Simone Picelli, et al. Nature Protocols, 2014). Smart-seq2