GSM7774439: organoid1; Homo sapiens; RNA-Seq
Organoids (cultured in the absence or presence of iMac for 18 days) were digested into single cells and washed once in 0.04% BSAsupplemented PBS. iMac cultured in the absence of organoids for 18 days were also subjectedto the same digestion and washing processes. The cells were then resuspended to a final cellconcentration of 530-1000 cells/μL in PBS/0.04% BSA. Using the 10X Genomics ChromiumController, about 8,700 cells were encapsulated in droplets at a targeted cell recovery of 5000cells, resulting in estimated multiplet rates of 3.9%. Single cell RNA-seq libraries were prepared using the 10X Genomics Chromium Single Cell 5′ v1 Library & Gel Bead Kit and i7 Multiplex Kit according to the manufacturer's protocol. The libraries were subjected to an indexed paired-end sequencing run of 2x151 cycles on an Illumina HiSeq 4000 (Illumina, San Diego, CA, USA) at a sequencing depth of 50,000 reads/cell.