GSM6856778: CD4+ T cells, 10 μM acacetin  treatment, biol rep 3; Mus musculus; RNA-Seq
The total RNA was extracted from the tissue samples, and the concentration and purity of the extracted RNA were examined using Nanodrop2000, the RNA integrity was examined by agarose gel electrophoresis, and the RIN value was determined by Agilent2100. A single library build required total RNA ≥ 1ug, concentration ≥ 35ng/μL, OD260/280 ≥ 1.8, OD260/230 ≥ 1.0 . Library construction using Illumina TruseqTM RNA sample prep Kit method