Whole genome sequencing of novel Hanseniaspora spp.
High molecular weight DNA was extracted and purified using the Qiagen Genomic Tip 100/G kit according to manufacturers instructions. Sequencing was performed from 400 ng DNA using the rapid barcoding kit (SQK-RBK004) from Oxford Nanopore Technologies (ONT), following the manufacturers instructions. Sequencing was performed on a MinION MK1B device (MinKNOW v. 19.05.0) using an r9.4.1 chemistry flowcell (FLO-MIN109). Basecalling was performed using guppy (v. 16.04.5) and reads were demultiplexed using qcat (v. 1.1.0).