RNA-seq of tomato
mRNA was purified from RIN7 total RNA samples with paramagnetic NEXTFLEX Poly(A) Beads 2.0 beads and after fragmentation, strand-specific library preparation was performed using the NEXTFLEX Rapid Directional RNA-Seq kit 2.0 kit. The completed pooled libraries are sequenced on the Illumina NovaSeq 6000 genome sequencing platform with 2x150 bp PE reads.