Transcriptome of the telencephalon of male Bluegill
RNA was extracted from each forebrain using a Trizol kit (Life Technologies) and the manufacturer's protocol. The quality of the RNA was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies) at the London Regional Genomics Centre, samples had RIN scores 8.2. RNA from 21 individuals, 7 from each treatment, was submitted to the London Regional Genomics Centre. Illumina TrueSeq Stranded mRNA Library Preparation Kits LT were used to build the cDNA libraries, which were sequenced on an Illumina NextSeq 500 High output using the paired end 2  150bp kits, 300 cycles. Each individual received a unique identifiable sequence. Time Analysis (v2.4.11) was used to call bases and score quality. Reads were converted to Fastq files with bcl2fastq2 (v2.20).