GSM7689388: RRBS_WT_3; Mus musculus; Bisulfite-Seq
Genomic DNA (total 500 ng) was first digested with Msp1 (NEB) at 37°C for 7 hours and then with ApeKI (NEB) at 75°C for 16 hours. The digested DNA fragments were purified using the MiniElute PCR Purification Kit (Qiagen). The reduced-representation bisulfite sequencing libraries were generated using the TruSeq Standed genomic DNA sample preparation kit.