GSM6753942: DMSP-iPSC-2; Mus musculus; RNA-Seq
ciPSC medium included KnockOut DMEM supplemented with 10% KSR, 10% FBS, 1% NEAA, 1% P/S, 0.055 mM β-ME, 20 ng ml-1 bFGF, and the small molecules VPA (0.5 mM), CHIR99021 (20 μM), 616452 (10 μM), Parnate (5 μM), Forskolin (50 μM), AM580 (0.5 μM), EPZ004777 (5 μM) and vitamin C (Vc) (250 μM). Total RNA was isolated and purified using FastPure Cell/Tissue Total RNA Isolation Kit (Vazyme). cDNA library construction and high-throughput sequencing were performed by Novogene.