16s rRNA of soil derived bacterial culture
Microbial community amplicon sequencing was performed using a sequencer (Illumina Miseq San Diego, CA) at the Center for Fundamental and Applied Microbiomics at the KED Genomics Core Facility, Arizona State University, Tempe, AZ [195, 196].  The primers utilized were 515F and 806R for the 16S rRNA V4 hyper-variable gene region of Bacteria and Archaea [201].  PANDAseq was used to pair forward and reverse reads [202].  Reads were processed using the Quantitative Insights into Microbial Ecology (QIIME 2.0 v. 2020.8) pipeline.  DADA2 was used for quality control and to truncate each sequence at 160 base pairs, maintaining a quality score of 25 or better to produce amplicon sequence variants (ASV).  A pretrained Naive Bayes classifier referencing the SILVA database (v.138) and the q2-feature-classifier plugin were used to assign taxonomy to amplicon sequence variants [203-206].  The BLAST + consensus taxonomy classifier plugin was used to compare study sequences against the National Center for Biotechnology (NCBI) database [207].