Hemlock (Tsuga) transcriptome sequencing
Needles were ground in liquid nitrogen and RNA extracted with CTAB buffer-based method and DNA removed. RNA was purified over RNA Clean and Concentrator-5 columns and accessed for quantity with Agilent Bioanalyzer before paired-end 150 bp sequencing on an Illumina NextSeq 2000 at the Georgia Genomics and Bioinformatics Core.