SNS-seq of Xenopus in vitro replication assay
Prior lambda exonuclease digestion, single-stranded NS DNA was treated with RNAse A and RNAse T2. Remaining DNA was converted into double-stranded DNA by random priming using DNA polymerase I (Klenow fragment). Heat-denatured sheared genomic DNA also was random primed before library preparation using the Illumina ChIP-seq DNA Sample Prep Kit, according to the manufacturers instructions.