Bile proteome reveals biliary regeneration during normothermic preservation of human donor livers
Bile proteome reveals biliary regeneration during normothermic preservation of human donor livers
Liver tissue biopsies were taken on the backtable (in the cold) directly prior to start of machine perfusion. RNA was isolated using Qiagen RNeasy Lipid Tissue Mini Kit, according to the manufacturer's protocol.  The RNA-seq libraries were constructed according to the Smart-3SEQ protocol using 100 ng of total RNA per sample as input.