Nanopore of Tiarella polyphylla
After the samples passed the inspection, Megaruptor was used to randomly interrupt the genomic DNA. Magnetic beads were used to enrich and purify large DNA fragments. The BluePippin automatic nucleic acid recovery instrument was used to cut and recycle large fragments. Repair fragmented DNA damage; After purification, end repair and A-plus ligation reaction were performed at both ends of the DNA fragment. After purification, LSK108 ligation kit was used for ligation reaction, and finally Qubit was used for accurate quantitative detection of the established DNA library