GSM6807938: retinal organoids derived from Homo sapiens fetal stem cells DRAM2 KO, rep1; Homo sapiens; RNA-Seq
Single-cell RNAseq libraries prepared using 10x protocol from eye organoids derived from DRAM2 WT (1 sample) and DRAM2 KO stem cells (1 sample). N=2 samples. Library was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics). Briefly, GCs were resuspended in the master mix and loaded together with partitioning oil and gel beads into the chip to generate the gel bead-in-emulsion (GEM). The poly-A RNA from the cell lysate contained in every single GEM was retrotranscripted to cDNA, which contains an Ilumina R1 primer sequence, Unique Molecular Identifier (UMI) and the 10x Barcode. The pooled barcoded cDNA was then cleaned up with Silane DynaBeads, amplified by PCR and the apropiated sized fragments were selected with SPRIselect reagent for subsequent library construction. During the library construction Ilumina R2 primer sequence, paired-end constructs with P5 and P7 sequences and a sample index were added.