GSM8155168: Hepatoblastoma sample 10; Homo sapiens; RNA-Seq
Ten fresh tumor samples from five patients were obtained for single-cell dissociation. Using scalpels, samples were minced into pieces 1-2 mm3 in size, followed by digestion in a papain solution containing papain (Worthington) in pre-warmed DMEM/F12 (Thermo Fisher) and 32.4 µg/ml DNAse (Worthington). After digestion, samples were passed through a 40 µm cell strainer (Corning) followed by red blood cell lysis using ACK lysing buffer (Thermo Fisher). Resulting single-cell suspensions were cryopreserved. After sorting for vital cells using 7-aminoactinomycin D (7-AAD, eBioscience) staining in flow cytometry with a BD FACSAria II Cell Sorter (BD Biosciences), single-cell suspensions were processed using Chromium Single Cell 3' Gel Bead Kit v3.1 (10x Genomics) and a Chromium Next GEM Chip G Single Cell Kit (10x Genomics) according to the manufacturer's protocol.