clone 45 APOBEC1+RBM47 +doxycycline
The RNA was extracted using the Directzol RNA miniprep kit Zymo Research), and RNA integrity was assessed by utilizing the Agilent 2100 Bioanalyzer RNA Nano assay (Agilent Technologies, Santa Clara, CA, USA). For direct RNA sequencing, RNA samples were processed into libraries using the ONT SQK-RNA002 kit, following the protocols provided by the manufacturer. Each library was subsequently loaded onto an individual R9.4.1 flowcell, and the sequencing runs were carried out using the ONT MinKNOW software (v3.4.12) to monitor and generate the data. The sequencing runs were terminated after 72 hours, and the resulting data was saved in FAST5 format.