RNA-seq to investigate sex-dependent changes in microglia in Cst7 knockout mice crossed with a mouse model of amyloid-driven Alzheimer's Disease
RNA-seq to investigate sex-dependent changes in microglia in Cst7 knockout mice crossed with a mouse model of amyloid-driven Alzheimer's Disease
Microglia were isolated by enzymatic digestion and fluorescence-activated cell sorting from brains of 12-month-old male and female mice. RNA was purified using RNEasy Plus Micro kits (Qiagen) according to manufacturerâ€™s instructions. RNA was pooled from 1-4 animals within the same experimental group. RNA within pools was equally distributed in amount between the animals. 1 ng of total RNA was taken forward for low-input RNA sequencing. cDNA was generated using TakaraBio SMART-Seq v4 Ultra Low Input RNA kit before input into the Illumina Nextera XT library preparation.