GSM8207625: IR64_flag_leaf_H3K27me3_1; Oryza sativa Indica Group; OTHER
Take 100mg of fresh flag leaf, use scissors or a blade to chop the flag leaf until homogenized. Resuspend tissue samples with 20 ml 1×PBS (+1% 50x protease inhibitor).Filter homogenized solution through Miracloth (Millipore, cat. no. 3536029) into a new 50 ml centrifuge tube.Centrifuge at 600 g for 5 min at 4 °C and remove the supernatant.Resuspend product with 1 ml NEB2 buffer and transfer to a new 1.5 ml centrifuge tube. Centrifuge at 600 g for 5 min at 4 °C and remove the supernatant. The library was constructed using the CUT&Tag kit (Hyperactive® Universal CUT&Tag Assay Kit for Illumina, Vazyme Biotech Co.,Ltd).