GSM8290272: W1 PBMCs, lanes 1-4, ADT-derived cDNA; Homo sapiens; OTHER
After one hour rest, cells being used for cellular indexing of transcriptomics and epitopes by sequencing (CITEseq) were blocked at 1:20 with TruStain FcX (BioLegend) and FCR Blocking Reagent (Miltenyi Biotec) in staining buffer (PBS, 2% BSA, 0.01% Tween) for 10 minutes at room temperature. Following this, cells were stained with hashtag oligos (Biolegend) for 30 minutes at room temperature. Cells were then pooled, loaded onto Chromium HT Chips from the Chromium Next GEM Single Cell 5' HT Kit v2 and run on a Chromium controller (10X Genomics). Gene expression and V(D)J libraries were constructed with the 5' Feature Barcode Kit, Chromium Single Cell V(D)J Amplification Kit, and Chromium Next GEM Single Cell 5' Library Kit (10X Genomics) as recommended by the manufacturer. Libraries were pooled and sequenced using the NovaSeq 6000.