Med-SEP2022-60m-CCS PacBio CCS metagenomic sample
Med-SEP2022-60m-CCS metagenomic sample was collected at 60 m deep in the epipelagic Mediterranean Sea at 20 nautical miles off the coast of Alicante (Spain) (37.35361N, 0.286194W), in September 2022, when the water column is strongly stratified. 200 L were collected and sequentially filtered on board through 20-, 5-, and 0.22-m pore filter polycarbonate filters. Water was directly pumped onto the series of filters to minimize the bottle effect. Filters were immediately frozen on dry ice and stored at 80C until processing. DNA extraction was performed from the 0.22-m filter (free-living bacteria) following the MagAttract Purification Kit protocol (QUIAGEN). Metagenome was sequenced using PacBio Sequel II (one 8M SMRT Cell Run, 30-h movie). To improve the quality of the PacBio raw reads, we generated Highly Accurate Single-Molecule Consensus Reads (CCS reads) using the CCS v6 program of the SMRT-link package. The minimum number of full-length subreads required to generate a CCS read was set to 15 (> 99.99 % base call accuracy).