GSM8253606: SKNMC-sgGFP_ChIP_H3K4me1_7d_REP1_230320_TAGCTT_S10; Homo sapiens; ChIP-Seq
In brief, cells were fixed in either 1% Formaldehyde (FA) for 10 minutes (histone modifications) or with 2uM DSG (30 mins) following by 1% FA (10 mins, for non histone proteins). Following fixation, cells were lysed and sonicated. Beads and antibody complexes were incubated with sonicated lysis overnight. ChIP material was washed 3x ChIP wash buffer and 1x TE+50mM NaCl. DNA-rpotein complexes were eluted from beads and de-crosslinked overnight. The following day, DNA clean up was performed using column purification. 1-10 ng of ChIP DNA was used for the library preparation using the SMARTer ThruPLEX DNA-Seq Kit (Takara) ChIP-Sequencing was performed using the Illumina Next Gen Sequencing NextSeq platform (Illumina, San Diego, CA) with 37bp, paired-end reads.