GSM6753459: day5, scRNA-seq; Mus musculus; RNA-Seq
Cells were dissociated into single cells with 0.25% trypsin. The dissociated cells were washed twice with 0.04% BSA in PBS and resuspended to a concentration of 1000 cells/uL. Then the cells were subjected to single-cell RNA sequencing. Library was performed according to the manufacturer's instructions (Chromium Next GEM Single Cell 3' Library Kit v3.1,10x genomics).