Sequel II sequencing; Raw reads: 7143
HMW-DNA (120 ng/µl),was sheared with Megaruptor2 with setting of 20-25kb. Five µg of sheared DNA was used for library construction with the HiFi SMRTbell Express Template Prep Kit 2.0  and SMRTbell Enzyme Clean Up Kit 2.0 .  When multiplexed, libraries were combined in equal amounts and size-selected with the BluePippin system (SageScience) with 10 kb cutoff in a 0.75% DF Marker S1 High-Pass 6-10kb vs3 gel cassette (BLF7510, Biozym). The library pool was sequenced in a single SMRT Cell (30 hours movie time) following the loading and pre-extension recommendations  with the Sequel II system (Pacific Biosciences) using the Binding Kit 2.2.