GSM6856731: Liver metastasis, subject 663, sample ID 26; Homo sapiens; RNA-Seq
For patients, Input RNA quantity was 1μg except for Sample ID 15 (0.5μg) and 27 (0.24μg) due to low volume.  For cell lines, total RNA inputs of 1μg were used to generate libraries using the New England BioLabs NEB Ultra II Directional RNA Library Prep for Illumina with rRNA Depletion kits. For patients, RNA-Seq libraries were constructed using the Illumina TruSeq Stranded Total RNA Library Prep kit with IDT xGen Dual Index UMI Adapters.  For cell lines, 200ng aliquots of each library were pooled and used as input for cDNA-Capture. The IDT xGen Exome Research Panel v2 including 415,115 exon probes was used for exome capture. Total RNA-Seq for patient samples, cDNA-Cap RNA-Seq for cell lines.