Full-length transcriptomic RNAseq of E. gracilis
Total RNA was extracted from approximate 1x108 E. gracilis cells. An Iso-Seq library was prepared according to the Isoform Sequencing protocol using the Clontech SMARTer PCR cDNA Synthesis Kit and the BluePippin Size Selection System protocol as described by Pacific Biosciences (PN 100-092-800-03). Subsequently, A full-length transcriptomic RNAseq was performed on the PacBio Sequel platform by Novogene (Beijing, China).