GSM7993583: HUCCT1-DMSO-rep1; Homo sapiens; RNA-Seq
Total RNA from cell samples was extracted with RNA Extraction Reagent (Vazyme, China) according to the manufacturer's instructions. Total RNA samples were stored at -80℃. The quality was evaluated by a 4200 TapeStation system (Agilent, USA) and quantified using a Life Invitrogen Qubit 3.0 fluorometer (Thermo Fisher Scientific). Then, the samples were used for library preparation. The mRNA with polyA structure was captured by Oligo (DT) magnetic beads. Fragmented mRNA was used as the template to synthesize the first strand of cDNA in the M-MuLV reverse transcriptase system. Then RNA was degraded by RNaseH. The second strand of cDNA was synthesized in the DNA polymerase I system. The double-strand cDNA was then purified and added by polyA tails and adaptors followed by PCR amplification. The product of PCR was purified by AMPure XP beads.