Bacillus probiotic ANA40
Established protocols for isolating strains of Bacillus sp., and for the preparation and storage of seed cultures intended for probiotic production. These seed cultures have a shelf life of 3 years when stored at -30C. As a result, 40 strains of Bacillus sp. were individually preserved in tubes containing a medium supplemented with 20% glycerol, all maintained at -30C. Additionally, 100 vials of the first seed culture for each of the following five Bacillus sp. strains B. subtilis ANA46 and B. coagulans ANA40were prepared and stored under the same conditions for future production purposes