RNA-seq of Homo sapiens:7AKNA1
. mRNA was purified with oligo dT-linked magnetic beads from total RNA. Then, mRNA was fragmented using divalent cations and temperature following the recommendations of the Illumina kit (Illumina, San Diego, CA) and operating conditions of the sequencing unit of the Instituto Nacional de Ciencias Genmicas (INMEGEN). A reverse transcriptase reaction was then performed on the mRNA fragments to generate cDNA libraries. Adapters were incorporated into the cDNA fragments by ligation.