Illumina NovaSeq 6000 paired end sequencing; Single-cell RNA-seq data of cardiac cells derived from a human induced pluripotent stem cell control line
Single-cell RNA-seq data of cardiac cells derived from a human induced pluripotent stem cell control line
After 30 days of hiPSC cardiac differentiation, for each sample, cells were harvested from three distinct beating wells, dissociated using the Multi Tissue Dissociation Kit 3 (Miltenyi Biotec), and pooled. Cell suspensions were filtered on a 40 µm Flowmi® Cell Strainer, counted and cell viability was assessed. For each sample, single-cell droplet libraries were generated from 16000 cells with the Chromium Single Cell 3ʹ GEM, Library & Gel Bead Kit v3 (10X Genomics). For each sample, single-cell droplet libraries were generated from 16000 cells with the Chromium Single Cell 3ʹ GEM, Library & Gel Bead Kit v3 (10X Genomics).