GSM7034552: MDPC_2; Rattus norvegicus; miRNA-Seq
Total RNA was isolated from the samples by using the exoRNeasy Midi/Max Kit (Qiagen, USA) according to the manufacturer's protocol. The extracted total RNA was analyzed by an Agilent 2100 Bioanalyzer (Agilent Technologies, USA). After the total RNA was quantified, the sequencing RNA library was constructed with the Small RNA Sample Pre Kit, which included the following steps: addition of a 3'-end linker, addition of a 5'-end linker, reverse transcription, amplification, cDNA library size selection, and purification. Qubit 2.0 was used for preliminary quantification, and then, an Agilent 2100 was used to measure the insert size of the library. After qualified library inspection, the library was pooled for Illumina SE50 sequencing according to the requirements of effective concentration and target offline data amount. The sequencing process was controlled by the data collection software provided by Illumina that performed real‐time data analysis.