GSM8155705: ATAC_JMURTK2_Mock_NT2_24h; Homo sapiens; ATAC-seq
Cells were lysed and the transposition reaction was performed with the Tn5 Transposase (Illumina, FC121-1030) at 37°C for 30 min. The reaction liquid was purified using the MinElute PCR Purification Kit (Qiagen, 28004). Five cycles of PCR were conducted using custom Nextera PCR primers 34 and NEBNext Q5 Hot Start HiFi PCR Master Mix (NEB, M0543S). The number of additional PCR cycles was determined by qPCR of the partly amplified products. The PCR products were purified using Agencourt AMPure XP beads (Beckman Coulter, A63881), using double size selection (left ratio: 1.4x; right ratio: 0.5x) according to the manufacturer's protocol. The samples were sequenced using the MGI Tech DNBSEQ-G400R sequencer, with 150 bp paired end reads.