PrimalSeq-LibAmp tiled amplicon sequencing of SARS-CoV-47
PrimalSeq-LibAmp Version 2.0. This protocol is adapted from https://www.nature.com/articles/nprot.2017.066. The general approach is to amplify SARS-CoV-2 genomes in small (~250 bp) overlapping fragments using two separate, highly multiplexed PCR reactions. The amplicons are combined after PCR for library preparation and paired-end 150 nt sequencing. Protocol oriented towards NovaSeq or NextSeq, but should be compatible with any Illumina sequencer.