18S rRNA of environmental samples Lake Lunz (Austria)
18S rRNA sequences obtained from amplicon sequencing analysis of the experimental community collected in 2020. The sample was first amplified using 18S rRNA primers targeting the V7 hypervariable region (F-1183mod, 5- AAT TTG ACT CAA CAC GGG-3, R-1443mod, 5- AAG GGC ATC ACA GAC CTG-3, Hadziavdic et al., 2014). Subsequently, the sample was sequenced at Eurofins Genomics Germany GmbH (Ebersberg, Germany) using Illumina NovaSeq sequencing with 2x150 paired-end reads. Sequences were matched to their taxonomy using the PR2 database (Guillou et al., 2013).