GSM7695914: Two-Donor-SSNseq-CAR-T-ex-vivo-5P, TCR-derived cDNA; Homo sapiens; OTHER
Lentiviral barcoded human primary CAR T cells were pooled and loaded into a Chromium Controller (10x Genomics). 10x Chromium Next GEM Single Cell 3' Reagent Kits v3.1 or 5' Reagent Kits V2 (with Feature Barcoding technology for CRISPR Screening) was used to construct the mRNA, small RNA and/or TCR libraries, according to the manufacturer's protocol (CG000316_RevD or CG000510 Rev B).