GSM7994996: A129_6_1; Mus musculus; ncRNA-Seq
RNA was extracted using a QIAGEN RNA kit or off-site at Novogene (for sRNA extraction). Small RNA quality was checked prior to library construction, and samples of small RNA without degradation and genomic DNA contamination were considered appropriate for use. Three micrograms of total RNA from each sample were used for library construction with the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA). Briefly, the 3' adapter was first ligated to the small RNAs, followed by ligation of the 5' adapter. Then, the ligation-reconstructed small RNAs were reverse transcribed into small cDNAs. After amplification by PCR, a library with a length of 140~160 bp was purified by gel excision. Library quality was checked on an Agilent Bioanalyzer 2100 system before sequencing. Small RNA sequencing was conducted on the NovaSeq 6000 SE50 platform, and at least 10 M clean reads were obtained for each sample.