RNAsec of Control 1: human male
Raw images generated by the sequencer are submitted to analysis, per-cycle basecalling and quality score assignment with Illumina's Real Time Analysis integrated primary analysis software (RTA v2). Conversion of BCL (base calls) binary files to FASTQ format is subsequently performed with bcl2fastq2 from within Local Run Manager (Illumina).  Fastq represents each sequence read by four lines (Sequence identifier, Sequence, Quality score identifier line and Quality score). Qscores are formatted in the standard Sanger encoding (ASCII with offset of 33). . Delivered sequences have passed Illumina quality filtering to remove least reliable reads (PF, for Passing Filter). Depending on primary analysis settings different result files may be included for each sample, complete files with partial results; files to be concatenated to include all results in the analysis: .. When present, different *_L00?_* files come from physical partitions of the same library in the flowcell from which reads are collected; with "L00?" (=Lane #) differing from "L001" to "L004". Each "L00?" file contains a different set of reads, which need to be concatenated for analysis.