GSM8329509: Lacrimal gland, CD25KO female mouse #6918, 8 weeks of age; Mus musculus; RNA-Seq
A Qiagen Plus Mini Kit was used to extract RNA from total excised lacrimal gland lysates, per manufacturer protocol. A double-stranded DNA library was created at Baylor College of Medicine's Genomics and RNA Profiling Core. Briefly, using 100ng of total RNA (measured by pico green), an oligodT primer containing an Illumina-compatible sequence at its 5' end was hybridized to the RNA, and reverse transcription was performed using a Lexogen kit. Second-strand synthesis was initiated by a random primer containing an Illumina-compatible linker sequence at its 5' end. The purified double-stranded library was then amplified and purified. The resulting libraries were quantitated using Qubit 2.0 (ThermoFisher) and fragment size was assessed with the Agilent Bioanalyzer (Agilent Technologies, Santa Clara, California).