GSM6481479: A549NA, scRNAseq; Homo sapiens; RNA-Seq
Irradiated A549 cells were harvested after continued culturing for 2 h and 6 h after irradiation. For scATAC-seq, irradiated A549 cells were harvested immediately and after continued culturing for 2 h after γ-ray treatment. Non-irradiated A549 cells for scRNA-seq and scATAC-seq were both harvested at 2 h after radiation. Single-cell sample preparation was conducted according to Sample Preparation Demonstrated Protocol provided by 10x Genomics as follows: 1mL of cell suspensions from each sample was pelleted in Eppendorf tubes by centrifugation (400 g, 5min). The supernatant was discarded, and the cells pellet was then resuspended in 1x PBS with 0.04% BSA, followed by two washing procedures by centrifugation (150 g, 3min). After the second wash, cells were resuspended in ~500 uL 1x PBS with 0.04% BSA followed by gently pipetting mix 10-15 times. Cells were counted using an Invitrogen Countess automated cell counter (LUNAⅡ) and the viability of cells was assessed by Trypan Blue staining (0.4%). After cell harvest, library and sequencing were completed by JingNeng company. Libraries were prepared using the 10x Genomics Chromium Controller in conjunction with the single-cell 3′ v2 kit.