GSM7108289: tKO_r2_L1_exit [tKO_E1e24r2]; Homo sapiens; RNA-Seq
Total RNA was prepared with the Trizol reagent (Thermo Fisher Scientific) or phenol/chloroform method according to the manufacturer's instructions. TrueSeq 500-1000 ng of total RNA isolated from ETV1 KO, tKO, and WT hPSCs to prepare libraries with the TruSeq RNA Library Prep Kit v2 according to the manufacturer's protocol. Libraries were prepared in duplicate. Libraries were quantified with a Qubit fluorometer (TFS), and their quality was assessed with the Agilent High Sensitivity DNA kit (Agilent Technologies, USA). Libraries were sequenced with an Illumina HiScanSQ sequencer.