GSM6755020: PTX activation, control, rep 3; Rattus norvegicus; RNA-Seq
RNA isolation was carried out according to the improved method of RNA phenol-chloroform extraction [Toni et al. MethodsX 2018, doi:10.1016/j.mex.2018.05.011]. To reverse transcribe RNA and amplify full-length cDNA, TeloPrime Full-Length cDNA Amplification kit V2 (Lexogen, Austria) was used. For preparation of barcoded cDNA libraries, Native Barcoding Expansion 1-12 (EXP-NBD104) and Ligation Sequencing Kit (SQK-LSK109) (Oxford Nanopore Technologies, UK) were used. All kits were used according to the manufacturer's protocol.