Illumina HiSeq 4000 sequencing; RNA-seq of Arabidopsis SF3B145 mediated gene expression regulation
RNA-seq of Arabidopsis SF3B145 mediated gene expression regulation
Ten-day-old seedlings grown under long day (16 h/8 h light-dark photocycles) conditiond at 20-22 Â°C the sequencing reads were aligned to the Arabidopsis TAIR10 reference genome using Bowtie2 and Hisat2 Total RNA was extracted and purified using Trizol reagent (Invitrogen, Carlsbad, CA, USA)  Sequencing libraries were gener-ated using the NEBNext Ultra RNA Library Prep Kit for Illumina (E7530) (New England Biolabs, Ipswich, MA, USA)