GSM7792143: Nuclei from s_0142 sample; Homo sapiens; RNA-Seq
To identify the substantia nigra in Control and PD tissues, cryostat sections of 5μm thickness were cut, haematoxylin and eosin (H&E) stained and viewed under the microscope by a neuropathologist (DRT) to identify region populated with melanin containing neurons. Based on this observation, the substantia nigra pars compacta region was manually annotated with a waterproof pen. H&E-guided 2 mm biopsy punches were collected from the SNpc of selected samples and nuclei were isolated to prepare the sequencing libraries. Libraries were prepared using the Chromium Single Cell 3 Reagent Kits v.3 according to the manufacturer's protocol (10x Genomics).