GSM6531134: ArginineRep1; Eggerthella lenta DSM 2243; RNA-Seq
Qiagen Rneasy Mini Kit, with an additional 50s bead-beating step after lysozyme digestion to better disrupt cell walls Illumina's Stranded Total RNA Prep Ligation with Ribo-Zero Plus kit (#20040529) and 10bp IDT for Illumina indices. Supplementary oligonucleotide probes specific to E. lenta rRNA and other highly expressed noncoding RNAs were incorporated during Ribo-Zero depletion