GSM6858857: WT-YAP; Homo sapiens; RNA-Seq
Cells were dissociated to a single cell suspension using 0.05% trypsin and sorted for live cells using DRAQ7. Single cells were captured for sequencing library preparation using a 10X Chromium (10X Genomics, Pleasanton, CA) instrument at the BUMC Single Cell Core. Single-cell RNA-seq libraries were prepared according to the Single Cell 3' v2 Reagent Kits User Guide (10X Genomics). Sequence libraries were constructed using the Chromium Single-Cell 3' Library Kit (10X Genomics). Sequencing libraries were loaded on a NextSeq500 (Illumina) to obtain a sequencing depth of~50K reads per cell. 3445 cells from WT YAP, 2539 cells from YAP5SA were captured and sequenced.