Bulk WGS of Subclone 2 of Bridge Population 1a
Genomic DNA was purified using the DNeasy Blood and Tissue kit (Qiagen) and was then fragmented on a Covaris M220 instrument according to the manufacturers protocol. Libraries were prepared using Swift S2 Acel reagents on a Beckman Coulter Biomek i7 liquid handling platform from approximately 200 ng of DNA with 14 cycles of PCR amplification. DNA libraries were quantified on a Qubit 2.0 Fluorometer (Life Technologies) and fragment size distributions were evaluated on a Agilent TapeStation 2200 (Agilent Technologies). Pooled libraries were further evaluated with low-pass sequencing on an Illumina MiSeq and then sequenced to approximately 5X mean genome coverage on an NovaSeq 6000 instrument (Illumina) with 2 x150 bp paired end configuration in the Molecular Biology Core Facilities at Dana-Farber Cancer Institute.