genome seq of mus musculus
Genomic DNA isolated from tail DNA was sheared with an S220 Ultrasonicator (Covaris, Woburn, Massachusetts) and subsequently prepared using a KAPA LTP library preparation kit ( KAPA Biosystems, Wilmington, MA 01887). After hybrid capture, amplification, and quality assessment, the samples were sequenced on Illumina platform using a 100-bp paired-end protocol.