GSM7857182: Myo7a_Cochlea_WT_P30_Rep3; Mus musculus; RNA-Seq
Tissues were homogenized in 350ul of RLT buffer + DTT using a pestle until the tissue was no longer visible by eye. The homogenized lystate was mixed with 350 ul of 70% ethanol and then applied directly to the Qiagen RNeasy Micro Kit, according to the manufacturer's instructions and RNA was eluted into 15 ul of dH20. RNA quantity was established using a Nanodrop spectrophotometer and RNA integrity number (RIN) was calculated using a BioAnalyzer. All samples had a RIN score greater than 9.1. Preparation of the mRNA library was performed using poly A enrichment and sequenced on the Illumina NovaSeq sequencer using paired-end 150 bp reads.