GSM6531300: NAcc, FMT, Coc, Control2; Mus musculus; RNA-Seq
RNA was harvested using Rneasy mini kit (Qiagen). 1 ug of total RNA was used for the construction of sequencing libraries. DNAse-treated RNA were prepared with TruSeq Stranded Total RNA LT Sample Prep kit from Illumina. Poly-A RNA was purified and fragmented before strand specific cDNA synthesis. cDNA were then A-tailed and indexed adapters were ligated. After adapter ligation, samples were PCR amplified and purified with Ampure XP beads, then validated and quantified again using Agilent 2100 Bioanalyzer and Qubit fluorimeter. Then, cDNA libraries were sequenced on an Illumina HiSeq 2500 platform using SBS v3 reagents.