GSM7697240: cortex, MEK-4, experiment; Mus musculus; RNA-Seq
scRNA-Seq: Briefly, mouse embryos were dissected out and immediately submerged in fresh ice-cold Hanks balanced salt solution (Gibco 12175-095). The cortex was then cut into pieces and dissociated into a single-cell suspension using a Papain Cell Dissociation Kit (Miltenyi Biotec, catalog no. 130-092-628) according to the manufacturer's instructions. scRNA-Seq: The Chromium dropletbased sequencing platform (10X Genomics) was used to generate scRNA-Seq libraries, following the manufacturer's instructions (manual document part number: CG00052 Rev C). The cDNA libraries were purified, quantified using an Agilent 2100 Bioanalyzer, and sequenced on an Illumina NovaSeq 6000 or Illumina Hiseq4000. RNA-Seq: The E14.5 cortex tissues from Rosa-fgf8/Rosa-mek1dd mice and littermate controls were dissected. RNA-Seq: Total RNA was isolated using an RNeasy Mini Kit (QIAGEN) according to the manufacturer's protocol. RNA-seq libraries were prepared according to the Illumina HiSeq 4000 protocol. The gene expression level was reported with fragments per kilobase of exon model per million mapped reads (FPKM)