Whole genome sequencing of Haloarcula hispanica AKS155 (pyrF trmB::trmB-HA)
Culture was innoculated from a single colony into rich media (23% salt YPC with 50ug/mL uracil) and grown to mid-log phase at 37C. Genomic DNA was extracted using phenol-chloroform-isoamyl alcohol (25:24:1; Fisher Scientific) and ethanol precipitated. Purified DNA was quantified using a NanoDrop system (Thermo Scientific) and sonicated in a Diagenode sonicating water bath for 20 cycles on high. DNA quality was assessed by using a bioanalyzer using a high-sensitivity DNA chip (Agilent). Samples were submitted to Duke Center for Genomic and Computational Biology core sequencing facility for adapter ligation with TruSeq (Illumina) adapters and library amplification.