GSM6857977: Lgr5-tdT rep2; Mus musculus; ATAC-seq
Flow-sorted tdT+ cells (25,000 cells in duplicates) were lysed to prepare nuclear pellets which then underwent transposition with TDE1 Enzyme (Illumina, 20034197). Tagmented DNA was purified using Zymo DNA Clean and Concentrator-5 Kit (cat# D4014) and the purified DNA was PCR amplified with NEBNext 2x MasterMix and Illumina adapters (table S4). The libraries were purified post-PCR using AMPure XP beads (Beckman Coulter). 150-bp paired-end reads were sequenced on a Novaseq instrument (Illumina).