GSM8367230: B1-8 B cells; Mus musculus; RNA-Seq
Using single cell 3 'Library and Gel Bead Kit V3 (10x Genomics, 1000075) and Chromium Single Cell B Chip Kit (10x Genomics, 1000074), the cell suspension (300-600 living cells per microliter determined by Count Star) was loaded onto the Chromium single cell controller (10x Genomics) to generate single-cell gel beads in the emulsion according to the manufacturer's protocol. According to the manufacture's introduction, Single-cell RNA-seq libraries were constructed using Single Cell 3' Library andGel Bead Kit V3.