GSM7035909: HPB [1017]; Ovis aries; RNA-Seq
A representative sample of tissue (30 mg) from the center of the right liver lobe was ground using a mortar and pestle with liquid nitrogen and homogenized using a hand-held homogenizer. Total RNA was extracted following the manufacturer's instructions using Qiagen RNeasy Mini Prep Kit (Qiagen, Calif., USA). The NEBNext Ultra RNA Library Prep kit for Illumina (New England Biolabs, Mass., USA) was used for library preparation with Poly-A selection, according to the manufacturer's recommendations