RNA-seq of L. reuteri: Tetracycline
The RNA isolation and purification were performed using the Zymo Research Fungal/Bacterial RNA Prep Kit. Cell lysis was carried out using a Roche MagnaLyzer instrument. The quality of the RNA was assessed using an Agilent Bioanalyzer instrument, and ribosomal RNA (rRNA) was depleted using the Illumina Ribo-Zero kit. The resulting RNA sample, enriched for mRNA, was then used as input for the initial step of the Kapa Biosystems RNA-seq library preparation kit.