Genomic sequencing of Coccidioides posadasii strain 3347
Genomic DNA from each sample was prepared for next generation sequencing using the Nextera DNA Flex Library Prep kit (Illumina). In brief, 500 ng of gDNA from each pooled competition experiment were used as input and tagmented (process to fragment and tag the DNA with adapter sequences) with Bead-Linked Transposomes. After washing, the tagmented DNA was amplified with unique combinations of i7 and i5 index adapters. Library qualities were assessed using an Agilent High Sensitivity DNA chip run on a 2100 Bioanalyzer Instrument. Equal molar amounts of each library were multiplexed to a final concentration of 5-10 nM.