GSM6858741: YAS5A_1; Homo sapiens; RNA-Seq
Total RNA from cultured cells was isolated using Qiagen RNeasy Micro kit. 1.3 ug of total RNA was used for the construction of sequencing libraries. Sequencing libraries were generated by a Ribo-Zero kit (Illumina). cDNA was synthesized and tagged by addition of barcoded Truseq adapters. Libraries were quantified using the KAPA Library Quantification Kit (KapaBiosystems) prior to amplification with Illumina's cBot.