GSM8254396: resistant, 4 [RNA-seq]; Homo sapiens; RNA-Seq
Genomic DNA (gDNA) and total RNA were extracted from snap frozen patient tumor and patient-matched normal tissue, and frozen PDX tumors preserved in RNALater using the QIAGEN AllPrep DNA/RNA Mini Kit and the Ambion mirVana miRNA Isolation Kit. RNA libraries were constructed using the NuGen Universal Plus mRNA-Seq Kit. Briefly, after fragmentation of total RNA and double-stranded cDNA generation using a mixture of random and oligo(dT) primers, the RNA libraries were constructed by end repairing, adapter ligation, strand selection, and PCR amplification. After RNA library construction, libraries were quantified for equal molar pooling and paired-end sequenced with a read length of 2×50 bp on the Illumina NovaSeq 6000 S2 platform.