GSM8118797: ST2hi memory CD4, HDM, ATAC, rep2; Mus musculus; ATAC-seq
5×10^4 cells were pelleted, washed with 50 μl 1× PBS, and lysed with 50 μl lysis buffer. The nuclei were re-suspended in a 40 μl transposition reaction with 2 μl Tn5 transposase (Illumina) to tag and fragment the accessible chromatin at 37 °C for 30 min. Fragmented DNAs were purified using the QIAGEN MiniElute PCR Purification Kit and amplified using 8-10 PCR cycles based on the amplification curve. The libraries were sequenced on an Illumina NovaSeq 6000 Sequencing System.