WGBS of Hordeum vulage subsp. Vulgare
DNA was isolated using the DNeasy Kit and sheared with Covaris. Libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit for Illumina and bisulfite treated with the EZ DNA-Methylation Lightning Kit by Zymo. The resulting 150bp paired end libraries were sequenced with Illumina Hiseq2000 and NovaSeq.