G_16S_CT22_4
Bacterial communities were determined by the amplication of V3-V4 hyper-variable regions of the 16S rRNA gene. he following primers were used: 5' CCTACGGGNGGCWGCAG (forward) and 5' GACTACHVGGGTATCTAATCC (reverse). The methodology for library preparation followed the protocol available from Illumina Support Center (ISC) with a slight modification (2Phanta Max Master Mix, Vazyme Biotech, Nanjing City, China was applied instead of Kapa Hifi Hot Start Ready mix).