GSM8069042: THP-1 cells, si-CLEC5A, CSE for 48h_3; Homo sapiens; RNA-Seq
Total RNA was isolated from lung tissues and cells using the SteadyPure RNA kit (AG21022, AG, China) following an established protocol After the construction of the library is completed, initial quantification is performed using Qubit2.0 Fluorometer to dilute the library to 1.5ng/ul. Then, Agilent 2100 bioanalyzer is used to detect the insert size of the library. Once the insert size meets expectations, qRT PCR accurately quantifies the effective concentration of the library (the effective concentration of the library is higher than 2nM) to ensure the quality of the library.