GSM7072827: 2818-RP-34; Cavia porcellus; RNA-Seq
Total RNA was isolated from culture cells using TRIzol Reagent (Thermo Fisher Scientific) and further purifed from the aqueous phase of TRIzol using RNeasy Plus Mini kit (Qiagen) and eliminated genomic DNA by in-column DNase I digestion. Total RNA quality was assessed using a 2100 Bioanalyzer (Agilent, Santa Clara, CA). At least 200 ng of DNase-treated total RNA with high RNA integrity was used to generate poly-A-enriched mRNA libraries, using KAPA Stranded mRNA sample kits with indexed adaptors (Roche, Indianapolis, IN). Library quality was assessed using the 2100 Bioanalyzer (Agilent), and libraries were quantitated using KAPA library Quantification kits (Roche). Pooled libraries were subjected to 75-bp paired-end sequencing according to the manufacturer's protocol (Illumina NovaSeq6000, San Diego, CA). Bcl2fastq2 Conversion Software (Illumina) was used to generate de-multiplexed Fastq files.