16S rRNA amplicons of Colne Estuary sediment enriched on simulated martian chemical environment and used as inoculum of a static bioreactor
The microbial 16S rRNA gene was amplified by Polymerase Chain Reaction using the universal 16S rRNA gene primers com1 and com2 (CAGCAGCCGCGGTAATAC (Schwieger and Tebbe, 1998) and CCGTCAATTCCTTTGAGTTT (Stach et al., 2001). The PCR reaction mixture contained (per 25 L) 1  PCRBIO Ultra Polymerase red mix (PCR BIOSYSTEMS, United Kingdom), 0.4 M forward and reverse primers, and 5 ng of DNA. The PCR products were sequenced using the Illumina Miseq Platform by Molecular Research LP (Texas, USA)