GSM7110297: 2i_B9_IgG_S17; Mus musculus; OTHER
DNA from Cut&RUN samples were extracted using phenol-chloroform extraction as described in Janssens & Henikoff 2019. DNA concentraction was measured using Qubit (ThermoFisher Scientific, Q32854). CUT&RUN libraries were prepared using the NEBNext Ultra II DNA Library Kit for Illumina Sequencing according to Liu, 2019. 6 ng of total DNA was used for library preparation. Libraries were amplified using 9-12 cycles on the thermocycler. Post amplification libraries were size selected at 150-350bp in length using AMPure XP beads. Libraries were quantified by Qubit and validated at the Agilent Fragment Analyzer 5200.