GSM7232628: MS_MGD_1551; Homo sapiens; ATAC-seq
50,000 freshly isolated cells per line were treated with Tn5 transposase. ATAC-seq libraries were prepared according to established protocol (Corces, 2017). 50,000 freshly isoated cells per fibroblast line was used as input. After purification and PCR amplification for library generation, the libraries were double-sided bead purified using AMPure XP beads (Beckman, A63881) to remove contaminating primer dimers. All libraries were quantified on the Qubit 4.0 and library quality was assessed using Agilent High Sensitivity DNA Tapestation (Agilent, 5067-5584) before multiplexing and sequencing on a Nextseq 550 for Libraries  to a minimum of 40 million paired-end reads per sample with 75bp length.