GSM8255183: RIMD2210633_1; Vibrio parahaemolyticus; RNA-Seq
The total RNA in bacteria was stabilized by RNAprotect® Bacteria Reagent (Qiagen) and then was extracted using RNeasy Mini Kit (Qiagen). From extracted total RNA, ribosomal RNA was removed and the remaining RNA was fragmented, using the Ribo-Zero Plus rRNA Depletion Kit (Illumina). RNA libraries were prepared using TruSeq stranded mRNA Library Prep Kit (Illumina) without the Purify and Fragment mRNA step.