GSM6529492: Roots, protoplasted, rep 2; Solanum lycopersicum; RNA-Seq
Total RNA was extracted using the Direct-zol RNA Miniprep kit (ZYMO). Bulk RNAseq libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD (Lexogen). Barcoded libraries were then pooled together, and PE 150-bp reads were sequenced on the NovaSeq 6000 instrument (Illumina) at the UC Davis DNA Technologies Core.