miRNA-Seq of D1 chick subcutaneous adipose tissues
The cDNA libraries were prepared and the steps of miRNA cDNA library construction were the following: Ligated with 3 and 5adapters (Illumina, San Diego, CA, USA) using T4 ligase (NEB, England, ), purified RNA was reversetranscribed into the first strand cDNA and amplified by PCR using primers complementary to the adaptor sequences. The final small RNA sequencing library was prepared by purified the nucleotide fractions at 140~150 bp length. After then, each library was loaded into a single Illumina Hiseq (Illumina, San Diego, USA) lane with 75 bp single end sequencing.