GSM8366763: NGP_H2AK119ub; Homo sapiens; ChIP-Seq
ChIP assays were performed as previous papers (Orlando V et al., 1997, Methods, Fujimura Y et al., 2006, Development). Cross-linked chromatin prepared from the indicated cells was precipitated by each antibodies. NEBNext Ultra II DNA Library Kit for Illumina (NEB) was used to generate libraries from samples. All procedures were performed according to the protocols suggested by the manufacturers. The concentration of libraries was measured by Qubit 2.0 Fluorometer with Qubit dsDNA HS Assay Kit (Invitrogen).