GSM8330714: Organoid_ATF3-/-KRAS_repl 1; Mus musculus; RNA-Seq
RNA was isolated from pancreatic tissue and isolated acinar cells 22 days after initial TX gavage using Trizol (ThermoFisher Scientific, 15596018) and the PureLink RNA mini kit (ThermoFisher Scientific, 12183018A). RNA from organoids was isolated using RNeasy Mini Kit (Qiagen, cat #74104). Illumina NextSeq High Output 150 cycle sequencing kit was used for library making following rRNA reduction.