GSM7518970: AM17_AM_Media; Homo sapiens; RNA-Seq
Samples were thawed, mixed with chloroform, and the aqueous layer plus 50% ethanol was processed using miRNeasy Mini Kit with DNA digestion by DNAse I. AM.MDM.Mtb libraries were prepared using Clontech SMARTer stranded v2 Pico Mammalian kit and sequenced on a NovaSeq6000 SP flow cell. MDM.IFN libraries were prepared usingNEBNext Ultra II Directional RNA Library Prep Kit and sequenced on a NovaSeq6000 S4 flow cell. AM.cryo libraries were prepared using Clontech SMARTer stranded v2 Pico Mammalian kit and sequenced on a NovaSeq6000 S1 flow cell.