GSM7916546: Th2_replicate.1; Mus musculus; OTHER
DNA was extracted from 1x10^6 CD4 Th cells/ replicate using the Qiagen DNA mini kit according to manufacturer's instructions (Qiagen Hilden, Germany). DNA was sheared using g-tubes (Covaris, USA) to ~10KB before library preparation with ligation sequencing kit (ONT Cat #SQK-LSK109) followed by whole genome sequencing on Nanopore MinION flow cells with pore 9.4.1 chemistry for 72h. Libraries were enriched using Cas9-targeting approach for a panel of 10 genes.