ChIP-seq of Arabidopsis thaliana using H3K27me3 antibody: mea-1;dme-2 homozygous Seedling Replicate 2
The NEBNext Ultra II DNA Library Prep Kit for Illumina was used to construct ChIP-seq libraries.  50-200 ng of ChIP DNA was further fragmented to 250-300 bp, followed by End prep and adaptor ligation. Excess adapter was removed using sample purification beads, PCR enrichment of adapter ligated DNA and clean-up of PCR products was performed as instructed by the manufacturer. The cleaned libraries were quantitated on Qubit fluorometer and appropriate dilutions loaded on a High sensitivity D1000 screen tape of a Tapestation 4200 (Agilent) to determine the size range of the fragments and the average library size.