GSM7995402: cspg4-cKO_spinalcord_EAE_3; Mus musculus; RNA-Seq
Total RNA was extracted utilizing the RNeasy Mini Kit (Qiagen; Cat# 74104) and its concentration was determined using a Nanodrop 2000 spectrophotometer and a Qubit fluorometer (both from ThermoFisher Scientific). The quality of the extracted RNA was evaluated using a 2100 Bioanalyzer (Agilent). For bulk transcriptomics of EAE-affected spinal cords, library preparation was performed using the SureSelect Strand-Specific RNA Library Prep System (Agilent; Cat# G9691) following the manufacturer's protocols. These samples were sequenced on the NovaSeq 6000 System (Illumina), achieving a sequencing depth of over 30 million 2x150bp paired-end reads.