GSM8328972: h- nmt41-GST-pab1-KanMx6 igo1::natMx6 cells, pab1 repression, igo1 mutant, minimal medium w/o nitrogen 4 hrs, rep 2; Schizosaccharomyces pombe; RNA-Seq
Cells were washed with cold DEPC-H2O and snap freeze. RNA extraction was carried out by disrupting the cells with cells with glass beads in TRIzol® Reagent (Invitrogen) and following the manufacturer's instructions. Library preparation using the Illumina TruSeq Stranded Total RNA and subsequent NGS sequencing was performed by Macrogen.