GSM8031792: AGS cells, Vector; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIZOL Reagent following the manufacturer's instructions and checked for a RIN number to inspect RNA integrity by an Agilent 2100 Bioanalyzer. According to the experimental instructions, the purified Total RNA was subjected to the steps of mRNA separation, fragmentation, first-strand cDNA synthesis, second-strand cDNA synthesis, terminal repair, 3' terminal A addition, linker connection, enrichment, etc., to complete the construction of sequencing sample library.