GSM7036203: StimulatedsnRNA5B; Homo sapiens; RNA-Seq
Multiomic (snRNA-seq+snATAC-seq) Nuclei were isolated from brain tissue using the Chromium Nuclei Isolation Kit. Nuclei were directly processed for droplet capture for single cell multiome ATAC + gene expression using a chromium controller. Chromium Next GEM Single Cell Multiome ATAC + Gene v1 chemistry was used to create single nuclei ATAC and RNA libraries from the same cell. Two baseline and two stimulated samples were used for independent replicates. Libraries were sequenced on an Illumina Novaseq 6000 with a 150 bp paired end read setup.