GSM7518449: RN_brain_Saline; Rattus norvegicus; RNA-Seq
Nuclei extraction buffer (Miltenyi) and discontinuous iodixanol gradient separation were used to generate single nuclei suspensions, in accordance with manufacturers' protocols. Libraries were prepared using Chromium Next GEM Single Cell 5´GEM, Library & Gel Bead Kit v5.2, Chromium Next GEM Chip K Single Cell Kit and Single Index Kit TT Set A (all 10x Genomics, Pleasanton, CA, USA), as per standard manufacturers' protocols.