GSM7233200: np2; Homo sapiens; miRNA-Seq
RNA was isolated from 500μl serum using proprietory protocol optimised for serum/plasma. RNA was eluted in ultra-low volume followed by QC analysis of RNA Library preparation done using QIAseq miRNA library Kit. 5μl total RNA was converted into microRNA NGS libraries. Adapters containing UMI were ligated to RNA, then RNA converted to cDNA. cDNA was amplified using PCR an indices were added. After PCR samples were purified Library preparation QC performed using Bioanalyser 2100(Agilent) or TapeStation 4200 (Agilent). Library pools were quantified using PCR. microRNA library pools were then sequenced using NextSeq500 sequencing instrument acording to manufacturer's instructions.