rRNA sequencing : bacterial community in polystyrene microplastic biofilm (without chlorination)
Biomass sample used for NGS was collected from polystyrene microplastic biofilm (without chlorination). The biomass was used for the RNA extraction. The obtained total RNA was converted to cDNA. The cDNA was used as template for high throughput sequencing. A universal bacteria primer set (i.e., 341F and 805R) was used to construct the library.