Shotgun sequence of sheep rumen microbes
Shotgun metagenomic sequencing was carried out using NovaSeq 60 0 0 system (Novogene, Singapore). The default specification of online server Metagenomic Rapid Annotation Subsystem (MG-RAST) was used for the structural analysis and functional annotation of the sequenced data. After quality assessment, sequenced data were annotated using a BLAST-likealignment algorithm called BLAT, against M5NR database [6] which offers concise alliance with many other databases