MFT with NAE at day 0
Total genomic DNA was extracted from triplicate 0.5 g subsamples taken from each replicate microcosm (each replicate) using the optimized protocol developed for FFT (Young et al., 2020), and then amplified using universal primers for Bacteria and Archaea (An et al., 2013). Extracted DNA from three replicates under each treatment were pooled. Purified PCR products were sent to The Applied Genomics Core at University of Alberta (Edmonton, Canada) where the second round of amplification was conducted using Illumina bridge PCR-compatible primers followed by sequencing using an Illumina MiSeq platform