GSM7036215: WT_PS_STAPUT_ChIP_SETX; Mus musculus; ChIP-Seq
WT pachytene spermatocytes used for ChIP-seq were isolated by a BSA dradient method. Setx-KO germ cells were purified by enzyme digestion method. Genomic DNA was fixed with formaldehyde and fragmented by sonication, followed by antibody immunoprecipitation. The immunoprecipitated DNA fragments were subjected to sequencing library preparation using the Illumina TruPLEX DNA-seq kit. The prepared libraries were purified by AMPure XP magnetic beads.