GSM7073444: oxaliplatin + oxybutynin treated enteric neurons, rep 1; Homo sapiens; RNA-Seq
Cells were dissociated into single cell suspensions by accutase treatment (Stemcell Technologies, 07920) for 30 minutes at 37 °C, 5% CO2. Single nuclei suspensions were prepared in duplicate per condition according to 10x Genomics Isolation of Nuclei for Single Cell Sequencing Demonstrated Protocol. Briefly, dissociated cells were lysed on ice in lysis buffer for two minutes and washed with nuclei wash and resuspension buffer and resuspended to 1000 nuclei/µL in nuclei wash and resuspension buffer. Cells were strained to remove cell debris and large clumps. 10x Genomics Chromium Next GEM Single Cell 3' Reagent Kits v3.1 User Guide