GSM6858588: wt colonic organoid +TNF, H3K4me3; Mus musculus; ChIP-Seq
Organoids were cross-linked with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a ultrasonicator, and histone-DNA complexes were isolated with antibody. 5 ng of DNA was used as starting material for input and ip samples. Sequencing library was prepared with GenNext NGS Library Prep Kit (Toyobo, LPK-101) from 5 ng for a final sequencing depth of 40, 40, and 60 million reads per input, H3K4me3, and H3K27me3, respectively.