GSM8253992: Ctrl_2; Mus musculus; Bisulfite-Seq
1 μg of genomic DNA was subjected to library synthesis using TIANamp Genomic DNA Kit (TIANGEN, Beijing, China) After genomic DNAs were extracted from the samples, DNA concentration and  integrity were detected by NanoDrop spectrophotometer and Agarose Gel  Electrophoresis respectively. The DNA libraries for Enzymatic Methylation  sequencing were prepared as described previously. Briefly, genomic DNAs were  fragmented into 100-300bp by Sonication (Covaris) and purified with MiniElute PCR  Purification Kit（QIAGEN）. Purified DNA is enzymatically converted using a  EpiArt®DNA Enzymatic Methylation Kit.The fragmented DNAs were end repaired  and a single “A” nucleotide was added to the 3' end of the blunt fragments. Then the  genomic fragments were ligated to methylated sequencing adapters.