DuplexSeq of Homo sapiens:TK6 cells from liver-on-chip
~1000 ng of DNA was ultrasonically sheared to a mean fragment size of approximately 300 base pairs (bp). The DNA was then end-repaired, A-tailed and ligated to DuplexSeq Adapters and PCR-amplified prior to undergoing two sequential rounds of hybrid capture enrichment with TwinStrands 48 kb Human Mutagenesis panel of a design previously described (Cho et al 2023). Prepared libraries were sequenced on a NovaSeq 6000 (Illumina, San Diego CA, USA) to achieve a mean duplex depth of 33,311 (range 14,759 - 39,412) to yield a mean of 2.18 (range 0.97  2.59) billion informative duplex bases per sample