GSM8254206: vehicle,1; Homo sapiens; OTHER
Genomic DNA (gDNA) and total RNA were extracted from snap frozen patient tumor and patient-matched normal tissue, and frozen PDX tumors preserved in RNALater using the QIAGEN AllPrep DNA/RNA Mini Kit and the Ambion mirVana miRNA Isolation Kit. gDNA libraries were constructed using the Roche NimbleGen SeqCap EZ Human Exome Library Kit v2.0, which captures 64 Mb of the human genome by 2.1 million oligonucleotide probes for coverage of more than 20,000 genes in the human genome (Roche NimbleGen, Inc., Madison, WI). Briefly, after fragmentation of gDNA, the libraries were constructed by end repairing and A-tailing the fragmented DNAs, ligation of adapters, and PCR amplification.