GSM7035977: PD15 D14; Homo sapiens; RNA-Seq
High Pure RNA Isolation Kit (Roche) was used for RNA extraction from cultured cells. Total RNA from the wild-type and GLI2mut heterozygous  iPSC cells at d0, gut tube (D5) and endocrine progenitor (D14) stage of pancreatic differentiation (PD) were extracted from two independent biological replicates. Total RNA concentration and RNA integrity of each sample were determined with NanoDrop and Qubit 4 Fluorometer. RNA library preparations and sequencing reactions were conducted by the company GENEWIZ using standard Illumina protocols. Briefly, data was generated with an Illumina HiSeq 2x150 PE HO configuration.