GSM7995050: Input-1; Homo sapiens; ChIP-Seq
Dermal fibroblasts (1 × 10^7 cells) were fixed with 1% formaldehyde at room temperature for 10 minutes, quenched with 125 mM glycine and harvested. ChIP-seq assays, including purification of the enriched DNA, were performed using the ChIP-IT High Sensitivity Kit (#53040, Active Motif, Carlsbad, CA, USA). In brief, chromatin was isolated by adding lysis buffer and fragmented by sonication. DNA was sheared to an average length of 200–500 bp with a EpiShear probe sonicator (#53051, Active Motif, Carlsbad, CA, USA). The input DNA was purified with PCR Purification Kit (#28104, QIAGEN Miami, FL, USA).