GSM8366231: NP21010 scRNAseq; Homo sapiens; RNA-Seq
Nasal polyp and tonsil tissues were collected at surgery, placed in PBS-Tween with protease inhibitors, and processed in RPMI with 10% fetal bovine serum and 1% penicillin-streptomycin. They were fragmented, treated with DNase I and collagenase, dissociated, strained, and RBCs were depleted before preparing single-cell suspensions for flow cytometry, sorting, or 10x genomics, with cell viability confirmed by trypan blue staining. Library was peformed according to the manufacter's instructions (single cell 3' v2 protocol, 10xGenomics). Single cell suspensions was mixed with the master mix and loaded together with partioning oil and gel beads into the chip to generate the GEM.