GSM8031455: PDC098, TCR-seq; Homo sapiens; OTHER
iRepertoire Bulk Reagent Universal User Manual (V20200818). The starting material was 500ng RNA per sample. We used 9 barcodes - HTAIvc kits (HTAIvc01, HTAIvc02, HTAIvc03, HTAIvc04, HTAIvc05, HTAIvc06, HTAIvc07, HTAIvc08 and HTAIvc09). We pooled one library from each of the barcoded kits together for each sequencing run. The libraries were pooled with 10% PhiX spike-in and sequenced with NextSeq High Output 300 Cycles kits (Illumina) on an Illumina NextSeq 550 (read 1: 155 cycles; read 2: 155 cycles). Five total sequencing runs were conducted.