GSM8118951: GSC 810- ATAC-seq-replicate1; Homo sapiens; ATAC-seq
ATAC-seq was performed as previously described (Buenrostro et al., 2013) . A total of 50.000 cells per sample were used as input. In order to minimize PCR bias an aliquot of each ATAC-seq library was first subjected to five cycles of amplification to determine by quantitative PCR the suitable number of cycles required for optimal library amplification. Samples were then subjected to a dual size selection (0.55x–1.5x) using SPRIselect beads (Beckman Coulter, B23317). Fragment distribution of libraries was assessed with an Agilent Bioanalyzer