GSM7233003: CAPRIN eCLIP rep 2 (input); Homo sapiens; RIP-Seq
Cells were lysed and and sonicated in eCLIP lysis buffer, and RNA was fragmented using RNase I (Ambion) and RBP:RNA complexes were immunoprecipitated with RBP-specific antibody (A301-902A) RNA was reverse transcripted with Superscript III, and adaptor was ligated with T4 RNA Ligase 1, then libraries were constructed with Q5 High-Fidelity 2x Master Mix (NEB)