GSM7108806: Participant 22, Stress session, Time 3; Homo sapiens; RNA-Seq
Whole blood samples were repeatedly collected via an IV catheter into the antecubital vein. Blood samples collected in 10mL EDTA tubes were immediately centrifuged for 10min at 1500g prior to collection of plasma. Peripheral blood mononuclear cells (PBMCs) were then isolated through density-gradient centrifugation using Ficoll. 1 ug of total RNA per sample was extracted using QIAamp RNA Blood Mini Kit (Qiagen) and samples were delivered to Penn State College of Medicine's Genomic Facility where RNA quality was verified using the Agilent Bioanalzyer, followed by library construction. Library preparation was done using the QuantSeq 3'mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen) supplemented with UMI (unique molecular index) as per the manufacturer's instructions.