HiFi sequencing of Helianthus tuberosus: young leaf
High-quality genomic DNA was extracted from leaf tissue, sheared into 10-15 kb fragments by Covaris g-TUBEs, enriched and purified by AMPure PB magnetic beads, and transformed into SMRT libraries by SMRTbellTM Express Template Prep Kit 2.0 and SMRTbell Enzyme Cleanup Kit, and finally recycled and checked by BluePippin and Agilent 2099 Bioanalyzer.