Recovery of complete genomes of canine parvovirus from clinical samples
The MinION library preparation was performed using a Ligation Sequencing kit SQK-LSK-109 and Native Barcoding kits EXP-NBD104 and EXP-NBD114 (Oxford Nanopore, Oxford, UK). The resulting library was loaded on a R9.4 Oxford MinION flowcell (FLO-MIN106) and sequenced using a MinION Mk1B device. The ONT MinKNOW software was used to collect raw data. High-accuracy base-calling of raw FAST5 files and barcode demultiplexing were performed using Guppy (v6.0.1).