RAD-seq of Yesso scallop: BC farm
DNA was extracted from adductor muscle sections of Yesso scallop, purified and quantified by fluorometry, then normalized to 20 ng/ul and used as an input to double-digest restriction site associated (RAD)-sequencing library preparation. Genomic DNA was digested using PstI and MspI cutting enzymes, and size selected. Samples were individually barcoded and multiplexed at 32 individuals per chip. Multiplexed libraries were sequenced on an Ion Torrent Proton. Fastq data were trimmed for adapters and low quality sequences, then demultiplexed and truncated to a consistent size (80 bp), then uploaded to NCBI.