GSM8290856: ami-UPP-4 knockdown at control condition rep 2; Arabidopsis thaliana; RNA-Seq
RNA was extracted from leaves or leaf discs using the NucleoSpin® RNA Plant Kit (Macherey-Nagel) according to the manufacturer's instructions. 50 mg of fresh plant material was used for this purpose. The RNA was eluted from the column with 40 μl of RNase-free ddH2O Library construction was perfomed by Novogene Sequencing Service. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection RNASeq library of poly-A enriched transcripts