GSM7996267: 129CAS_PSC_2; Mus musculus domesticus x Mus musculus castaneus; RNA-Seq
motor neurons were processed using RNAqueous™-Micro Total RNA Isolation Kit (Thermo Fisher Scientific); PSCs were processed using RNAeasy-Micro Total RNA Isolation Kit (Thermo Fisher Scientific); for both cell types samples were treated on-column with RNase-Free DNase Set (QIAGEN, Hilden, Germany) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.