GSM6529120: RDE-1; Caenorhabditis elegans; RIP-Seq
Immunoprecipitated RNA was extracted with TRIzol reagent according to the manufacturer's instructions. RNAs were treated with RNA 5' polyphosphatase (Lucigen, Cat# RP8092H) and purified with RNA clean up kit (Zymo Research, Cat# R1015) for RNA libraries. Adaptor ligation, Unique Molecular Identifier (UMI)-mediated reverse transcription and library amplification were prepared according to manufacturer's instructions