GSM7697808: COX2452A21,Uninfected_Prdm1 fl/fl Maf fl/fl CD4-Cre,3; Mus musculus; RNA-Seq
On Day 14 following infection with Helicobacter hepaticus, colon lamina propria leukocytes were isolated from individual  infected and uninfected control mice and CD4+ T cells were sorted. RNA was extracted using the QIAShredder and RNeasy Mini Kit with on-column DNase digestion, according to the manufacturer's instructions (Qiagen). RNA-seq libraries were then made with total RNA using the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina and unique multiplexing indexes, according to the manufacturer's instruction (New England Biolabs).