GSM7110355: Sample 1; Mus musculus; RNA-Seq
Cells were dissociated in Accutase for 3 minutes at 37°C, and then the enzyme was inactivated by diluting in PBS and spinning 3min at 400rcf. Single cells suspension was obtained by repeatedly washing with 10 volume of ice-cold washing buffer (PBS- 10% FBS). Samples were flow sorted using AriaIII (BD Biosciences, BD Diva Software) cell sorter. 3' end mRNA libraries were prepared for sequencing using MARS-seq2 protocol (Keren-Shaul et al., 2019) single cell RNA-seq for gene expression quantitation