GSM7697370: o/eRNase H1 CIN612 cells_2; Homo sapiens; RNA-Seq
RNA was extracted using an Rneasy mini plus kit (Qiagen). RNAseq-PolyA technique was applied by Admera Biosciences. Briefly, PolyA+RNA was captured and then fragmented and primed for cDNA synthesis. Following cDNA synthesis, 3' ends were adenylated and 5' ends repaired. DNA sequencing adapters were ligated and the ligated fragments were PCR amplified.