GSM7036182: BulkRNAsubject5FemaleBaseline; Homo sapiens; RNA-Seq
Bulk RNA-seq Total RNA was extracted from sampled human brains using miRNeasy Mini Kite and homogenized in Qiazol using stainless steel beads. Chloroform was then used for phase separation. RNA containing an aqueous layer was further purified using the RNeasy MinElute spin column. RNA was finally eluted in RNase-free water. For bulk RNA-seq librairies were prepared using Ribo-Zero gold sample preparation kit. Library concentrations were measured using KAPA Illumina Library Quantification Kit. Polled libraries were sequenced on Illumina NovaSeq6000 sequencer with 150-bp Paired-End chemistry.