A new Bacillus thuringensis with toxicity against many lepidoptera pests
Genomic DNA was sheared using the Covaris g-TUBE device according to the manufacturer specifications . After fragmentation, DNA was evaluated with the TapeStation System with the Genomic DNA assay and also with the Agilent 2100 Bioanalyzer System with the Agilent DNA 12000 assay . Both of these methods have minimal sample consumption and return both sizing and quantitation. The sheared gDNA sample input was normalized for all samples between 15 g into library construction for PacBio SMRTbell 10 kb Library Preparation.