GSM7885431: BL6, vehicle treated bone marrow derived macrophages, rep1; Mus musculus; RNA-Seq
Total RNA from macrophages treated as described in Figure legends was isolated using Qiagen RNAeasy Plus Mini kit The Quality of total RNA was assessed using Agilent BioAnalyzer 2100 for RNA integrity. Samples with RIN>8 were used for library preparations. Sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina, following the manufacturer's instructions. Libraries quality was evaluated using Agilent BioAnalyzer 2100 and the libraries were sequenced by a NovaSeq 6000 system (50 bp, paired-end)