RNA-seq of mus musculus: brain tumor immune microenvironment
CD45+ immune cell were selected from GBM tumor microenvironment following tissue dissociation and positive cell selection.  The Chromium Next GEM Single Cell 3 Reagent Kits v3.1 (Dual Index, 10x Genomics) was utilized to construct the cDNA sequencing libraries. The samples were multiplexed and prepared as per the manufacturer's instructions and sequenced by Illumina Novaseq 6000.