RNA-seq of Bombyx mori: Transovarial Infection replicate 1
Nosema bombycis spores were isolated from the silk (labial) glands of severely infected last instar larvae and purified using sucrose density gradient. The infection was given to last instar by feeding mulberry leaves coated with spores of Nosema (~1 X 10 6 spores/ml) and maintained in the laboratory to get adult infected moths and further allow them to mate. The eggs laid by infected moths were grown until the larvae reached 2nd instar stage. The larvae were then collected and total RNA was extracted by TRIzol method as well as mRNA was enriched for sequencing.