GSM7108205: 0.5 nM ZJ-101 sample, biological replicate 2; Homo sapiens; RNA-Seq
Cells were harvested from semi-confluent 10 cm dishes using scrapers and pelleted in falcon tubes. Cell pellets were briefly washed with ice-cold PBS prior to total RNA extraction via the Rneasy mini kit (Qiagen) per the manufacturers instructions. Libraries were constructued by Genewiz following poly-A selection from total RNA samples, all RNA content was analyzed on an Agilent TapeStation with RIN > 9.5 for all samples.