GSM7885396: OPM2, KRAS wildtype, replicate 1; Homo sapiens; RNA-Seq
For the RNA-Seq analysis, the OPM2 KRAS WT and the OPM2-derived KRAS A146T and G12A cells were seeded in triplicates at a density of 100,000 cells/ml. Three days after seeding, two million cells were pelleted and stored at -80°C. The RNeasy Mini kit (Qiagen) was used to extract the RNA and the quality was checked with the 2100 Bioanalyzer and the RNA 6000 Nano kit (Agilent). The RIN was ~10 for all samples. Libraries were prepared from 1000ng of total RNA using the TruSeq stranded mRNA kit (Illumina). Quantification was conducted with the Qubit 3.0 Fluorometer (ThermoFisher) and the 2100 Bioanalyzer with the DNA 1000 kit (Agilent). Pooled libraries were sequenced (single-end) on the NextSeq 500 (Illumina) using the High Output Kit v2.5 (75 Cycles).