RNA-seq of Yarrowia lipolytica that cultivated in YPD for 5 d rep3
The Y. lipolytica strain W29 was grown in YPD liquid medium supplemented with 0, 1 mM CuSO4 at 28 . Cells cultured for 5 and 6 days were harvested and frozen quickly using liquid nitrogen. Three replicates were performed for each sample. The following steps including total RNA extraction, library construction, and sequencing were performed by Frasergen Information Co., Ltd. (Wuhan, China). Total RNA was extracted from samples, with the concentration and purity detected by Nanodrop 2000 and RIN value determined by Agilent 2100. A total amount of 2 g total RNA was used for library construction and mRNA was enriched using poly-T oligo-attached magnetic beads. Qualified libraries were prepared as DNA nano-ball for sequencing, using MGI high-throughput sequencer (MGISEQ-2002).