GSM7885313: WT_lactobacillus_murinus_RNAseq_bio_rep_3; Ligilactobacillus murinus; RNA-Seq
Total RNA Extraction Total RNA was extracted from tissue samples, and its concentration and purity were measured using Nanodrop2000 Removal of rRNA, fragmentation of mRNA, reverse Transcription of cDNA, adaptor Ligation. Then, digestion of the Second cDNA Strand with UNG Enzyme Before PCR amplification, the second cDNA strand is digested with UNG enzyme, so that the library contains only the first strand of cDNA.