Trichoderma cf. simile WF8 Genome sequencing
The genomic DNA of the sample was extracted by SDS or STE, and then the purity and integrity of the DNA was detected by agarose gel electrophoresis, and quantified by Qubit. The qualified DNA samples detected by electrophoresis were randomly fragmented into fragments with a length of about 350 bp using a Covaris ultrasonic breaker. After processing the DNA fragments, the NEBNextUltra DNA Library Prep Kit for Illumina (NEB, USA) was used to complete the entire library preparation through steps such as end repair, A-tailing, sequencing adapters, purification, and PCR amplification. After the library construction was completed, Qubit 2.0 was used for preliminary quantification, the library was diluted to 2ng/ul, and then used Agilent 2100 to detect the inserted fragments of the library. After the insert size met the expectations, Q-PCR method was used to accurately determine the effective concentration of the library. The MD5 checksums for files are: DEhun.L350_FDSW22H000011-1r_1.fq.clean.gz:MD5_0383f45e7ea632b0c2ce6abcc3033897;DEhun.L350_FDSW22H000011-1r_2.fq.clean.gz:MD5_1bb9072e2bdfd3b848d120ab8955b133.