GSM7697314: DNMT1_CRISPRi_rep2; Homo sapiens; RNA-Seq
Total RNA was isolated using the RNeasy Mini Kit (Quiagen) with on column DNAse treatment following the manufacturer's instructions. Libraries for RNA sequencing were generated using Illumina TruSeq Stranded mRNA library prep kit (poly-A selection), optimized for long fragments, and sequenced on a Novaseq6000 (paired end, ~ 250 bp).