GSM7916698: aggregation cultured dediHeps, replicate 3; Mus musculus; RNA-Seq
Total RNA was prepared from hepatocytes, dediHeps, dediHep aggregates, dediHep-derived organoids, FIPC-derived organoids, ISC-derived organoids, and CLiPs using an ISOGEN II (Nippon gene, Tokyo, Japan) or RNeasy mini kit (QIAGEN, Venlo, Netherlands), according to the manufacturer's instructions. Detailed protocol is described in "CEL-Seq2: sensitive highly-multiplexed single-cell RNA-Seq (PMID: 27121950)".