LPS853 Liposarcoma cell line input ChIP-Seq
In brief, crosslinked cells were lysed and DNA was sheared on the Covaris E220 (Fill level 5, Duty Cycle 5, PIP 140, Cycles-burst 200, Time 22–26 min) to between 200 and 2,000 base pair fragments using a covaris water bath sonicator. ChIP DNA was used to generate sequencing libraries by end repair (End-It DNA repair kit, Biosearch Technologies ER0720), 30 A base overhang addition via Klenow fragment (NEB M0212L), and ligation of barcoded sequencing adapters. Barcoded fragments were amplified via PCR (KAPA HiFi MM, Roche 07958935001).