RNA-seq of Arabidopsis thaliana:circadian rhythms in whole seedlings
Total RNA was isolated from 100 mg of frozen ground tissues of whole plantlets. RNA was extracted with Maxwell 16 LEV Plant RNA Kit (Promega, Benelux BV) using Maxwell 16 AS2000 Instrument (Promega) according to the manufacturers instructions. Quality and purity of the samples were verified with a NanoDrop 2000 UVVis Spectrophotometer (Thermo Scientific, Loughborough, UK). The sequencing was performed at VIB Nucleomics Core (VIB, Belgium, www.nucleomics.vib.be). Library preparation was performed with TruSeq RNA Sample Preparation Kit (Illumina, United States) and the library was sequenced using the NextSeq High Output Kit in single end mode (1 X 75 cycles) (Illumina, United states).