Sistrurus catenatus fecal samples
Amplicons were indexed using Nextera XT indexes (Illumina) and purified. Libraries were then normalized at equal molarities (4M) and pooled. The pooled libraries were denatured using 0.2N NaOH and diluted to 10 pM. 15% spike-in of 10 pM Phi-X was added. The pooled libraries and Phi-X were sequenced on an Illumina MiSeq Sequencer using a v3 600-cycle cartridge for 2 x 300 bp paired-end read sequencing.