G_ITS_CT44_2
Fungal communities were determined by the amplication of Internal Transcribed Spacer (ITS2). Forward primer was used: 5'GCATCGATGAAGAACGCAGC (ITS3, without overhangs) with the reverse primer 5' TCCTCCGCTTATTGATATGC (ITS4, without overhangs). The methodology for library preparation followed the protocol available from Illumina Support Center (ISC) with a slight modification (2Phanta Max Master Mix, Vazyme Biotech, Nanjing City, China was applied instead of Kapa Hifi Hot Start Ready mix).