GSM7856752: SKmel147 shSCR, H4ac ChIPseq; Homo sapiens; ChIP-Seq
For YL1 and H4ac ChIP, SK-MEL-147 cells were cross-linked with 1% Formaldehyde for 10 min at room temperature. For ZNHIT1 ChIP, SK-MEL-147 cells were were double cross-linked with 0.25 M disuccinimidyl glutarate (DSG) for 45 min, followed with 1% formaldehyde for 10 min. ChIP was performed  as previously described (Fontanals-Cirera B, Hasson D, Vardabasso C, Di Micco R et al. Harnessing BET Inhibitor Sensitivity Reveals AMIGO2 as a Melanoma Survival Gene. Mol Cell 2017 Nov 16;68(4):731-744.e9) Libraries for ChIP-seq were done as previously described  (Fontanals-Cirera B, Hasson D, Vardabasso C, Di Micco R et al. Harnessing BET Inhibitor Sensitivity Reveals AMIGO2 as a Melanoma Survival Gene. Mol Cell 2017 Nov 16;68(4):731-744.e9)