GSM8290941: Retina_OIR-CIH_P18_rep2; Mus musculus; RNA-Seq
Fresh eyes (3 eyes from the OIR-Norm group, 3 eyes from the OIR-CIH group, all at PN18) were dissected on ice to isolate retinas immediately after enucleation. Retinal RNA was extracted using the NuceloSpin RNA kit (Macherey-Nagel) according to the Midi protocol in the manufacturer's instructions. RNA quality was confirmed using a Bioanalyzer RNA Analysis kit (Agilent). RNA sequencing libraries were prepared from 250 ng total RNA using the TruSeq Stranded mRNA Library Prep kit (Illumina)