GSM8208279: D. melanogaster hmr2, ovaries, rep1; Drosophila melanogaster; RNA-Seq
For the preparation of RNA-seq libraries, RNA was isolated from D. melanogaster Hmr2, D. simulans w501 and interspecific hybrid ovaries with TRIzol Reagent (Invitrogen). PolyA selection was done using an Dynabeads™ mRNA Purification Kit (Thermo Fisher Scientific). RNA-seq libraries were generated using the NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina (#E7760, NEB) and NEBNext Multiplex Oligos for Illumina (Index Primers Set 1, NEB #E7335, and Set 2, NEB #E7500) according to the manufacturer's instructions and sequenced on the Illumina NovaSeq 6000 SP platform (100 bp runs).