GSM8436619: P25 neocortex, Chd8 het female, snRNA-seq, rep2; Mus musculus; RNA-Seq
The cerebral cortex was dissected from four litter- and sex-matched pairs of postnatal day (P) 25 wild type and Chd8+/- mice, followed by removal of the medial cortex. These samples were flash-frozen in liquid nitrogen. All 8 samples were processed in parallel for nuclei isolation using the 10x Chromium Nuclei Isolation kit, then loaded onto the 10x Genomics Chromium Controller for single nucleus capture. Libraries were generated and indexed using the 10x Chromium Next GEM Single-Cell 3' kit with v3 chemistry, then sequenced using standard Illumina protocols on an Illumina NovaSeq 6000, with 28 cycles for read 1 and 90 cycles for read 2.