GSM7036551: DAA276_sgRNA_NovaSeq; Homo sapiens; RNA-Seq
Cells were collected, washed once with PBS and stained with Cell Hashing antibodies ot CellPlex followed by manuffacturer manual. Cells were resuspended in PBS containing 0.05% of BSA to the desired concentration and immediately load on the 10x Chromium controller for single-cell RNA-seq preparation. For single-cell library preparation, we followed the standard protocol of 10x Genomics. After the cDNA amplification, 50 ng of samples were separated for the sgRNA enrichment library preparation.