GSM7518899: CX45_rep2; Mus musculus; RNA-Seq
The total RNA of cells was extracted, followed the manufacturer's instruction of Trizol Reagent (Invitrogen). KAPA HiFi HotStart DNA Polymerase (Roche) was used to amplify the cDNA. The RNA-seq library was prepared using Nextera XT DNA Library Preparation Kit (Illumina), followed by the manufacturer's instruction.