GSM8436720: Control 1; Escherichia coli; RNA-Seq
2 mL of samples were centrifuged at 14,000 X g for 2 minutes to pellet the cells. Zirconia beads were used for cell lysis for 10 minutes using a bead beater instrument. Total RNA was then extracted with a RiboPure Bacteria RNA Isolation Kit AM1925 (Invitrogen, USA) according to the manufacturer's recommendations, including DNase treatment. Ribosomal RNA depletion was performed using the NEB rRNA Depletion Bacterial Module (New England Biolabs, USA). The cDNA library preparation for Illumina sequencing was executed using the NEBNext Ultra II Directional RNA Library Prep Kit and NEB Indexing Kit (New England Biolabs, USA). After adaptor ligation and amplification, the average library fragment size was 360 bp.