GSM6857410: Smooth Muscle, Nuclear Localization STUB1, Replicate 3; Homo sapiens; RNA-Seq
Cells were collected using trypsin and RNA was gathered using the Qiagen RNeasy kit (cat. 74004) Libraries were prepared according to Lexogen's instruction accompanying the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Cat. 015). Briefly, total RNA was reverse transcribed by oligodT priming, RNA template was removed, and the second strand was synthesized by random priming using DNA polymerase. After bead purification, library is amplified by PCR and sequences required for cluster generation are introduced. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Illumina HiSeq 2500 following the manufacturer's protocols.