1012.34 scATAC-seq
Single cell suspensions were stained with Total-seq A hashtags and Total-seq B surface marker oligotags followed by fixation with 1% formaldehyde and lysis with NP-40 substitute to retain mitochondrial DNA as described by Lareau & Ludwig et al., Nature Biotechnology 2021 (PMID 32788668) and Mimitou et al., Nature Biotechnology 2021 (PMID 34083792). Single Cell ATAC (Assay for Transposase Accessible Chromatin) using Chromium platform (10x Genomics) according to manufacturer's instructions.