GSM6529130: RNA-seq siCPSF73 rep 1 [re-used]; Homo sapiens; RNA-Seq
Six in vitro transcribed spike-in RNAs were added to the lysates (TRIzol, Thermo) followed by extraction of RNA according to the manufacturer's protocol. The RNA was then sonicated and used as input for RNA-seq or for further purification of 4sU-labeled RNA (the latter data not included here). Total RNA was purified prior to libary preparation using the miRNeasy Micro Kit (Qiagen) including DNase I treatment to remove genomic DNA. RNA-seq libraries were prepared for sequencing using standard Illumina protocols (TruSeq Stranded Total RNA Sample Prep kit).