GSM7230962: QO23110_Male_Oxygen; Mus musculus; RNA-Seq
Total RNA from sorted endothelial cells was isolated using the Zymo micro kit (Zymo Research, Irvine, CA). Sample concentration and quality checks were done using the Agilent Bioanalyzer. The libraries were prepped using the Takara SMARTer Total RNA Pico kit v3. The first 8 nt of Read 2 were UMIs followed by 3 nucleotides derived from the Pico v3 SMART UMI Adapter, both of which were trimmed.