GSM8031665: ND-646_4 [5107-MR-105_8]; Mus musculus; RNA-Seq
Cells were washed in PBS and pellets were flash frozen at -80°C. RNA was extracted with a Promega Maxwell RSC 16 using the Maxwell RSC simplyRNA Cells kit according to the manufacturer's instructions. 1 mg of total RNA was used for the construction of libraries with the New England Biolabs NEBNext® Poly(A) mRNA Magnetic Isolation Module and Ultra II Directional RNA Library Prep Kit for Illumina according to the manufacturer's instructions.  Dual-indexed libraries were pooled and sequenced at VANTAGE (Vanderbilt University Medical Center) on an Illumina NovaSeq 6000 (S4 flow cell) to a depth of approximately 25 million paired-end 150 bp reads per library.