16S rRNA of soil: restored peatland with low water level
Soil DNA was extracted from 1 g of soil from each sample using a Fast DNATM SPIN Kit (MP Biomedicals, CA, USA). The concentration and purity of DNA extractives were examined and sent to Shanghai Majorbio Technology Co., Ltd., China, for high-throughput sequencing. The primer pairs used to amplify the V3-V4 hypervariable regions of the bacterial 16S rRNA gene were 338F (5-ACTCCTACGGGAGGCAGCA-3) and 806R (5-GGACTACHVGGGTWTCTAAT-3).