P9082 cfDNA
Extracted cell-free DNA was end-repaired and A-tailed using the Kapa HyperPrep kit, followed by ligation with Oxford Nanopore Native Barcodes. Pooled multiplexed libraries were then cleaned with Ampure XP, after which they were End Repaired and A-tailed again with the Kapa HyperPrep kit. Ligation with the LSK110 AMX-F adapter was performed, before Ampure XP cleanup using SFB wash buffer and elution into Oxford Nanopore EB buffer. Libraries were loaded at 150fmol per flow cell.