GSM7917857: Staphylococcus aureus IFM15, replicate 2; Staphylococcus aureus; RNA-Seq
To harvest cells, cultures were centrifuged at 15,000 x g for 1 min. TRIzol™ Reagent (0.7532 mL) was added to bacterial pellets that had been stored at -80℃. RNA extraction was subsequently performed according to the manufacturer's instructions with the following modifications. To obtain RNA from S. aureus, we added glass beads and vortexed for 1 minute, before placing the sample on ice for 1 minute and repeating this process 10 times. Protocol for rRNA depletion using NEBNext rRNA Depletion Kit (Bacteria), TruSeq Stranded Total RNA Reference Guide (1000000040499 v00)