GSM8329308: WT Mock-treated seedling RNA Rep 1; Arabidopsis thaliana; RNA-Seq
Total RNA from frozen tissue was isolated using the RNeasy plant mini kit (Qiagen). Isolated RNA was treated with Ambion TURBO™ DNase (Invitrogen) using manufacturer's recommendations. 500 nanograms of DNAase-treated RNA from RNA Extraction was used as input for the RNA-Seq library prep. The QuantSeq 3' mRNA-Seq V2 Library Prep Kit with UDI (Lexogen) was used to make sequencing libraries with manufacturer's recommendations.