Metagenomic sequencing of environmental community using adaptive sampling
We used 200 ng of input DNA for the Oxford Nanopore Technologies Rapid Sequencing Kit (SQK-RAD004) We sequenced the prepared library using the MinION mk1b (ONT) on a r9.4.1 flow cell (FLO-MIN106D) for 8 hours. Half of the flow cell was designated for adaptive sampling; the other half sequenced normally (adaptive sampling off). We base called the reads using Guppy version 6.1.3 with the super-accuracy model (-c dna_r9.4.1_450bps_sup.cfg)