GSM6857824: ALPHA 1 - REPLICATE 3; Homo sapiens; RNA-Seq
RNA  was extracted from cell lines using the Pure LinkTM RNA Mini Kit (ThermoFisher, USA) and Trizol (ThermoFisher, USA) 1. Take a certain amount of total RNA samples, and use oligo dT beads to enrich mRNA with poly A tail;  2. mRNA molecules were fragmented into small pieces;  3. The fragmented mRNA was synthesized into first strand cDNA using random primers;  4. The second strand cDNA was synthesized with dUTP instead of dTTP;  5. The synthesized cDNA was subjected to end-repair and 3' adenylated. Adaptors were ligated to the ends of these 3' adenylated cDNA fragments;  6. Digest the U-labeled second-strand template with Uracil-DNA-Glycosylase (UDG) and perform PCR amplification;