GSM8032374: RNA-seq of wildtype mouse CD150 neg cells  #2; Mus musculus; RNA-Seq
RNA was extracted from CD150 negative cells in trizol using Zymo Direct-zol RNA Microprep Kit (Zymo Research, CA). Libraries were prepared from total RNA with the Ovation® RNA-Seq System V2 according to the manufacturer's instructions (Tecan, PN 7102-08). Paired end 100 cycle sequencing was performed on a NovaSeq 6000 (Illumina).