GSM8329667: BMDM_chipseq_k4me3-ph74; Mus musculus; ChIP-Seq
The chromatin from approximately 10 million cells (25-30 g) were used for histone modification ChIP and 30 million cells (~100 g) were used for the transcription factor ChIP. Cell pellets were sonicated to an average chromatin fragment of 300-400 bp. The following antibodies were used for each ChIP experiment based on instructions: 10 g anti-H3K4me3 (ab4729), 10 g anti-H3K27Ac (ab8580), 1:100 anti-IRF3 (D83B9) and 10 g anti-p65 (sc-372). 100L of Protein G dynabeads (10003D) were used for each ChIP. NEBNext Ultra II DNA Library Prep Kit for Illumina