GSM6481888: THP1+ERAA, rep2, d3; Homo sapiens; RNA-Seq
RNA was harvested using Rneasy mini plus kit (Qiagen). 1 µg of total RNA per sample was used as starting material. The NEBNext® Ultra™ II RNA Library Prep Kit (Cat. No. E7770), the NEBNext® Poly(A) mRNA Magnetic Isolation Module (Cat. No. E7490) and the NEBNext® Multiplex Oligos for Illumina® (Cat. No. E7335 and E7500S) were used for the library preparation of RNA samples according to the manufacturer's instructions. A deep sequencing of the RNA libraries (30x10E6 reads per sample) was carried out on the Illumina NextSeq 500