GSM7109138: ESC_TRE_DUX_WT_pLV2_2_DOX_rep2; Mus musculus; RNA-Seq
Total RNA was isolated using Isolate II RNA Mini Kit (Bioline). DNA libraries for RNAseq analysis were prepared using NEBNextUltraII Directional RNA Library Prep Kit for Illumina (New England Biolabs, NEB) and NEBNextrRNA Depletion Kit (Human/Mouse/Rat) (NEB) according to the manufacturer's protocol. Sequencing was performed on the Illumina NextSeq550 (75bp pair-end reads).