GSM8329499: right atrial appendage, post-operative AF, patient nr. 1246; Homo sapiens; RNA-Seq
Right atrial appendages were obtained from patients undergoing cardiac surgery. Tissues were immediately flash frozen in liquid nitrogennd and stored at -80 °C. Total mRNA was extracted from 40 mg of tissue with RNeasy Fibrous Tissue Mini Kit (Qiagen). RNA purity and yield were quality checked with Agilent Bioanalyzer to satisfy RIN >7 and total RNA amount 3-5 μg. TruSeq RNA Library Prep Kit (Illumina)