GSM8031802: CLL cells, CXCR4low, replicate 2; Homo sapiens; RNA-Seq
Total RNA was extracted from the CLL cells using TRIzol reagent, RNA purity and quantification were characterized using a NanoDrop 2000 spectrophotometer (Thermo Scientific, USA), and RNA integrity was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), and samples that passed quality control were used for subsequent library construction. Ribosomal RNA was removed using the Ribo-off rRNA Depletion Kit (Vazyme, Nanjing, China), and the transcriptome library was constructed using the VAHTS Universal V6 RNA-seq Library Prep Kit according to the instructions.