GSM8068893: VEH_01 [S448.01]; Rattus norvegicus; RNA-Seq
Total RNA was extracted using the Zymo Research Quick-RNA MicroPrep Kit per manufacturer's instructions, including DNase treatment. 1-2 testes from fetuses in the same litter were pooled for each RNA sample. The RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer's instructions (New England Biolabs, Ipswich, MA, USA), with polyA selection for mRNA enrichment.