GSM8403738: TLR9agonist_LW,scRNA; Mus musculus; RNA-Seq
Dorsal skin healed from large circular wound (diameter=1.8cm) and sprayed with PBS or  ODN2395 at Day 0,3,6 post wound were collected for the scRNA-seq at 2 days post scab detachment. The cell suspension was loaded into Chromium microfluidic chips with 3' (v2 or v3, depends on project) chemistry and barcoded with a 10× Chromium Controller (10X Genomics). RNA from the barcoded cells was subsequently reverse-transcribed and sequencing libraries constructed with reagents from a Chromium Single Cell 3' v2(v2 or v3, depends on project) reagent kit (10X Genomics) according to the manufacturer's instructions. Sequencing was performed with Illumina (HiSeq 2000 or NovaSeq , depends on project) according to the manufacturer's instructions (Illumina).