NGS for tob
A glycerol stock of P. aeruginosa TF mutant library was thawed and mixed on ice. Then, 100 l of cells were diluted into 100 ml of LB medium with or without 3 g/ml tobramycin. The culture was shaken at 37 overnight and harvested by centrifugation. The genomic DNA was extracted by the Ezup column bacteria genomic DNA purification kit (Sangon, Shanghai) and then subjected to NGS.