GSM8403742: WT, replicate 1_R1, scRNAseq; Homo sapiens; RNA-Seq
Midbrain organoids were dissociated using the Papain Dissociation System (Worthington Biochemical, USA). The cell suspension withh added 800 µL of freezing-cold 100% methanol were stored at -80°C until further analysis. Samples after rehydration were subjected to scRNA-seq using the 10× Genomics Chromium Single Cell 3' Gene Expression system with feature barcoding technology for cell multiplexing. Libraries were generated using 10x Chromium