GSM6529238: HeLa, Diagenode 20ug K27ac, 12.5M reads; Homo sapiens; ChIP-Seq
Cells were fixed with 0.75% formaldehyde for 5 minutes. Cells were quenched with 750mM Tris for 5 minutes. After cell lysis, chromatin was fragmented using micrococcal nuclease (worthington). Library preparation was done using KAPA HyperPrep Kit (Roche, KK8504) with 4 uL of Illumina adapters (IDT, UDI) and sequenced on Illumina NextSeq 500 with a Mid-Output (paired-end 75 bp reads) flow cell. Input libraries had between 83-102M reads, and each IP library had 27-46M reads that passed QC, with 88% of the bases having quality scores >= 30.