GSM7518875: hy5-2, SIM 8D, High light, replicate 3; Arabidopsis thaliana; RNA-Seq
Hypocotyl explants were frozen with liquid nitrogen and ground with beads by a TissueLyser II (QIAGEN). RNA extraction with DNase digestion was performed by the RNeasy Plant Mini Kit (QIAGEN) following the default instructions. Isolated RNA was then subjected for library preparation using the KAPA Stranded mRNA-Seq Kit (NIPPON Genetics) with NEB adaptors for Illumina (New England Biolabs) and AMPure XP beads (Beckman Coulter).