16S-DNA-Seq of soil:SW_FW_GS
Undried bulk soil samples were collected from experimental plot and genomic DNA extracted in triplets using PowerLyser PowerSoil DNA Isolation Kit (MO BIO Laboratories, Carlsbad, CA, USA) following the manufacturers protocol, modified by starting with initial soil mass approximately 0.5g and by use of a bead beater for cell lysing. The V4 16S region was PCR amplified using Illumina adaptor ligated universal primers  519F(5-CAGCMGCCGCGGTAA-3) and 786R(5-TACNVGGGTATCTAATCC-3) from each triplet. The thermocycler condition was maintained at 94oC for 3 min, followed by 25 cycles of 94oC for 30 s, 55oC for 30 s, 72oC for 30 s and a final extension of 72oC for 3 min.