GSM8253753: RC17 cells, FGF8/FGF17, day 16, rep 2, RNA, A2; Homo sapiens; RNA-Seq
Four batches of paired FGF8- and FGF17-treated day 16 VM progenitors (8 samples total) were thawed in wash media containing DMEM/F12 + 5%KOSR. Cells per sample were stained with 0.5µg of unique cell surface hashing antibody(Biolegend) and incubated for 30 min at 4 degrees. After antibody tagging, cells were washed three times in PBS(wo Ca and Mg) with 0.5%BSA and resuspended at 1000 cells/ul in PBS(wo Ca and Mg) +0.5%BSA The eight samples were prepared to be run across two 10X sequencing lanes by pooling two batches of paired FGF8 and FGF17 samples at 1:1:1:1 ratio for both sequencing lanes. 10X scRNAseq was combined with CITE-Seq for sample multiplexing to sequence eight different replicates across two protocols.