GSM8330754: K521R_Rep1; Homo sapiens; RNA-Seq
Extraction was carried out by Trizol method. Extracted RNA quantity is checked on Qubit flurometer (Thermofisher #Q33238) using RNA HS assay kit (Thermofisher #Q32851) following manufacturer's protocol. To measure the purity of the extraction we also measured the concentration of the samples on Nanodrop 1000. The library preparation was carried out using NEBNext Ultra II Directional RNA Library Prep Kit (NEB #E7760S/L). Final libraries were quantified using Qubit 4.0 fluorometer (Thermofisher #Q33238) using DNA HS assay kit (Thermofisher #Q32851) following manufacturer's protocol. Obtained results are presented below in the table. To identify the insert size of the library, we queried it on Tapestation 4150 (Agilent) utilizing high sensitive D1000 screentapes (Agilent # 5067- 5582) following manufacturers' protocol. All libraries pass QC parameters and will be further taken for RNA-sequencing. Libraries that display adapters will be cleaned on the day of sequencing to protect the library stability.