Amplicon sequencing of pooled PCR products of Solanum
Pooled PCR products from primers designed to amplify a set of fruit sugar-related genes in tomatoes; each sample is a pool containing 7-16 amplicons; pools were purified with a Zymo Clean & Concentrator 25 kit, eluted in 38 ul LTE buffer, and diluted to 30-60 ng/ul. 35 ul of the diluted sample was sequenced by the Massachusetts General Hospital DNA Core Complete Amplicon Sequencing Service (Cambridge, MA, USA).