GSM8367183: Triadimefon-24h_1µM_R1; Danio rerio; RNA-Seq
Embryos were transferred to 1.5 ml microfuge tubes (kept on ice), followed by the removal of as much liquid as possible, and the addition of 250 μl/tube of TRIzol reagent. Embryos were lysed with pellet pestles by performing 20 strokes until the tissue was checked to be sufficiently disrupted, to then flash frozen all the tubes in liquid nitrogen. Samples were stored at -80 ºC until shipped to the University of Liège for sequencing. Illumina mRNA stranded ligation Kit