GSM7518378: 6month cut; Mus musculus; RNA-Seq
Total RNA was extracted with RNeasy Plus Mini Kit (Qiagen).The integrity of the isolated RNA was verified using the Bioanalyzer RNA6000 pico kit (Agilent Technologies). Due to an RNA integrity value of the samples were less than 8, RNA-Seq libraries were prepared from 10ng of RNA using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina kit, NEBNext rRNA Depletion Kit (Human/Mouse/Rat) and NEBNext Multiplex Oligos for Illumina. Each library was sequenced on MiSeq (Illumina) with MiSeq Reagent kit V3 150 cycle (Illumina) by 75 base pair-end reads. Differentially expressed genes were determined by an exact test after normalization.