GSM7776435: Retina, Hypoxia; Mus musculus; RNA-Seq
Retinas were isolated with the Winkler method, and the vitreous was cleared in ice-cold 1X PBS. The retinas were then digested with the Worthington Tissue Dissociation kit to generate single-cell suspensions. These suspensions were fixed in methanol prior to sequencing and kept at -80°C. The samples were rehydrated immediately before loading onto a 10X Genomics chip to generate cell-bead suspensions (v3 chemistry). Library construction was performed according to 10X Genomics standard protocols. Single Cell 3' v3 chemistry