GSM6481881: LN-tet-AR, doxycycline, DHT0.1nM, ChIP-AR; Homo sapiens; ChIP-Seq
Cells were crosslinked with formaldehyde and then sonicated. DNA were immunoprecipitated with AR antibody and protein A beads. After reverse crosslinking, DNA were purified with Qiagen PCR purification kit. ChIP DNA Library were prepared with NEBNext ChIP-Seq Library Prep Reagent set for Illumina and NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1)