GSM6921680: DBA D1, cultured 3d, mRNA; Homo sapiens; OTHER
Cells were collected on days 0, 3, and 6.  Cells were washed and resuspended in PBS with 1% BSA at 5×10^5 per ml.  Cells were incubated with 10 μg/ml of each DNA-labelled antibody. Single-cell mRNA barcoding and library preparation were performed on the 10x Chromium controller according to the manufacturer's instructions. A single GEM (gel beads-in-emulsion) well from each sample was used to generate both the gene expression and antibody capture libraries which were recombined for sequencing. The libraries were sequenced on Illumina NextSeq 550 or NovaSeq instruments CITE-seq library preparation was performed according to the Biolegend Totalseq protocol.  (Biolegend TotaSeq or equivalent): CD3, CD11b, CD14, CD36, CD70, CD71/TfR, CD117/cKit, CD235a/GlyA. Following the final bead purification, all three libraries were pooled as 85% mRNA library, 10% ADT library and 5% PhiX Control library before sequencing.