GSM8290900: Lung E10.5 A; Mus musculus; RNA-Seq
WT male fetal mouse lungs were isolated at E10.5, E12.5, E14.5, E16.5, and E18.5 and processed for single cell as described (N=1). For samples isolated at E10.5 and E12.5, duplicates were obtained, one containing only one lung and the other containing three lungs. Samples isolated at later time points consisted of one single lung. The resulting cell suspensions were processed by the Erasmus Center for Biomics and/or Department for Hematology. Single-cell libraries were prepared using the Chromium Single Cell 3' Reagent Kit v3 (10x Genomics). Next-generation sequencing (28-8-0-91 cycles) was conducted on an Illumina NovaSeq6000 platform (Illumina). Raw data were processed into FASTQ files, and sequences were quality-checked using FastQC (version v0.11.5).