GSM6754371: Blood, not active, animal 11 [11_B_NA]; Ovis aries; RNA-Seq
Blood samples were taken in Tempus tubes (Applied Biosystems, Thermofisher, UK) and stored at -80 °C, The TempusTM Spin RNA Isolation Reagent Kit was used to extract the total RNA from peripheral blood (Applied Biosystems, Thermofisher, UK) according to the manufacturer´s instructions. Sequencing was carried out at the CNAG (Centro Nacional de Análisis Genómico, Spain; https://www.cnag.crg.eu/). The RNA-Seq library was prepared with KAPA Stranded mRNA-Seq Illumina Platforms Kit (Roche) following the manufacturer´s recommendations using Illumina platform compatible adaptors with unique dual indexes and unique molecular identifiers (Integrated DNA Technologies). The final library was validated on an Agilent 2100 Bioanalyzer with the DNA 7500 assay. The libraries were sequenced on NovaSeq 6000 (Illumina) with a read length of 2x151bp following the manufacturer's protocol for dual indexing.