Nanopore MinION sequencing of Spiraea crenata
We prepared the sequencing library with 1,000 ng of DNA following the recommendations of the Genomic DNA by Ligation Sequencing Kit (SQK-LSK110, Oxford Nanopore Technologies, Oxford, UK). We loaded a total of 600 ng of the prepared library onto an R10.3 MinION flow cell (Oxford Nanopore Technologies). We generated raw data for 72 hours using a MinION mk1b instrument, washed the flow cell after 36 hours using the Flow Cell Wash Kit (EXP -WSH003), and reloaded the library after refilling the flow cell. Raw MinION sequencing data were basecalled with Guppy 5.0.11 (Oxford Nanopore Technologies) using the Super High Accuracy basecalling model (dna_r10.3_450bps_sup) to achieve the highest possible accuracy per read.