GSM7697249: MycCap Tumors, IRE1A KO2, scRNA; Mus musculus; RNA-Seq
Freshly dissected tumor samples were washed with PBS and transferred into GEXSCOPE Tissue Preservation Solution (Singleron), and stored on ice. Six tumor samples from each group were pooled for scRNA-seq. The tissue samples were then washed with Hanks balanced salt solution three times and minced into small pieces which were digested with Tissue Dissociation Solution (Singleron) at 37 °C for 15 min with agitation. After digestion, cells were passed through a 40 μm strainer to remove cell debris and centrifuged at 300 × g for 5 min. The supernatant was removed and cell pellet was resuspended in 1 ml PBS, followed by red blood cell lysis using RBC lysis buffer. Next, cells were centrifuged at 500 × g for 5 min and resuspended in PBS. The single cell suspension was adjusted to a concentration of 1 × 105 / ml for library preparation. This was loaded onto microfluidics chips (GEXSCOPE Single Cell RNA-seq Kit, Singleron Biotechnologies) and GEXSCOPE 3´SD scRNAseq libraries were prepared according to manufacturer instructions (Singleron Biotechnologies).