GSM7031401: P07_WT_YL16; Mus musculus; RNA-Seq
Nuclei isolation protocol was modified from our previous work (Ayhan et al., 2021). Since homogenization of mouse cortex generated much fewer debris, we just did twice fltering through 40-μm Flowmi Cell Strainer (#H13680-0040, Bel-Art) without doing sucrose-gradient centrifugation The single-nuclei RNA-seq libraries were prepared using Chromium Single Cell 3ʹ Reagent Kits v3 (CG000185 Rev B) from 10x Genomics