Illumina HiSeq 4000 paired end sequencing; RNA-seq of KYAE-1 and OE19 oesophageal adenocarcinoma cells following induction of dominant negative (acidic) FOS
RNA-seq of KYAE-1 and OE19 oesophageal adenocarcinoma cells following induction of dominant negative (acidic) FOS
Polyclonal cells were generated by transducing cells with pINDUCER20-GFP-aFOS, followed by selection using G418. For RNA-seq, cells were seeded, and the following day treated with doxycycline to induce aFOS expression. 48 hours post-induction, RNA was harvested from cells.   RNA was extracted using the RNeasy Plus Kit (Qiagen) RNA-seq libraries were constructed using Illumina TruSeq Stranded mRNA kit