MP_21_016
mRNA was isolated from purified 50 ng total RNA using oligo-dT beads (New England Biolabs, Inc). NEBNext Ultra RNA kit was used for full-length cDNA synthesis and library preparation. Libraries were pooled, denatured and diluted, resulting in a 1.8 pM DNA solution. PhiX control was spiked at 1%. Libraries were sequenced on an Illumina NextSeq 500 instrument (Illumina Inc) using NextSeq 500 High Output reagent kit (Illumina Inc) (1x75 cycles). FASTQ files were generated using bcl2fastq 2.19.1 (Illumina).