GSM7863721: EHE-siGDF-15-1; Homo sapiens; RNA-Seq
Cells were lysed with QIAzol Lysis Reagent and RNA was extracted using QIAGEN miRNeasy kit according to manufacturer instructions. RNA stranded libraries were generated starting from 500ng total RNA and mRNA was selected with poly-T oligo attached magnetic beads using Illumina® Stranded mRNA Prep, Ligation protocol. Libraries were quantified and quality checked on an Agilent 4200 TapeStation system (High Sensitivity D1000), and then sequenced on an Illumina NovaSeq platform with paired-end reads 150bp long.