GSM7031842: 26-AK171-8hB2, tech rep 2, biol rep 2; Homo sapiens; RNA-Seq
RNA was extracted using phenol-chloroform extraction followed by clean-up using the RNAeasy Micro kit (Qiagen 74004). For transwell assays, library preparation was performed using NEBnext single-cell/low input RNA library prep kit (E6420L, NEB). For NKX2.2 CRISPR experiments, library preparation was performed using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB E7490L) integrated with NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB E7770L). RNA-seq; NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) oligos (NEB E7600S) was used for assigning indices to all samples prior to pooling. Pooled samples were run using an Illumina NextSeq 550 75 cycle high-output kit (Illumina 20024906). Samples were processed as single end reads (75:8:8).