RNA-Seq of Anomia simplex: tentacles
Tentacles isolated from live animals and preserved in RNAlater at -80C. Total RNA was extracted from pooled tentacles using TRIzol. The total RNA extracted was quantified using Qubit RNA Broad Range, and integrity was checked by running agarose gel electrophoresis. Quality assessment was performed on Agilent 2100 Bioanalyzer, and mRNA was isolated using NEBNext Poly(A) mRNA Magnetic Isolation Module. NEBNext Ultra RNA Library Prep Kit and NEBNext Multiplex Oligos for Illumina were used to generate the cDNA library and provide adaptor ligation, respectively.