GSM8330832: Uninjured_RGC_Itga5_Itgav_loci; Mus musculus; RNA-Seq
Adult uninjured or ONC-injured RGCs transduced with AAV2 expressing mCherry were Thy1-immunopanned from single cell retinal suspension (from 10 retinas per condition) and FACS'ed for mCherry+ cells. Approximately, 5,000 RGCs (per condition) were collected by FACS. RNA was isolated immediately using the Zymo QuickRNA microprep kit. Total RNA with RNA Integrity Number (RIN) ≥ 9 (by Bioanalyzer 2100 using the Nano 6000 kit, Agilent) was extracted using Direct-zol RNA MiniPrep kit (R2050, Zymo Research). cDNA libraries were prepared using polyA-selected RNA (TruSeq RNA Library Prep Kit, Illumina), and paired reads were sequenced 100 bp from each end on HiSeq 2000 Sequencer (Illumina).