GSM6533033: Hashtag CarT_Day14_1; Mus musculus; RNA-Seq
Endogenous CD45.2+ CD4 and CD8 T cells were sorted collectively into 1x RPMI +10%FBS, 2-5x104 total T cells were obtained for each group. Cells were pelleted at 1000xg for 5min, resuspended in 1xRPMI at 20,000 cells per 200μl and then processed for scRNA-seq using the Seq-Well platform with second strand chemistry, as previously described11. Whole transcriptome libraries were barcoded and amplified using the Nextera XT kit (Illumina) and were sequenced on a Novaseq 6000 (Illumina). Hashtag oligo libraries were amplified as described previously12 and were sequenced on a Nextseq 550. Seq-Well