GSM7697831: O157:H7 in media_3; Escherichia coli; RNA-Seq
Total RNA was extracted using RiboPure™ RNA Purification Kit (Invitrogen™). Bacterial RNA was separated from total RNA via mammalian RNA depletion using MICROBEn-rich™ Kit (Invitrogen™). Host depleted samples further processed for rRNA depletion to enrich bacterial mRNA using using MICROBExpress™ Bacterial mRNA Enrichment Kit (Invitrogen™). All steps were carried out according to the manufacturer's instructions. Quality and concentration of RNA samples in each step was measured using NanoDrop™ 2000/2000c Spectrophotometer (Thermo Scientific™). Library preparation was done using NEBNext Ultra RNA Library Prep Kit for Illumina as per manufacturer's recommendations