GSM8426917: BLISS_siNEAT1_Etoposide_rep1; Homo sapiens; OTHER
First, the DSBs in the genome were blunted, followed by adapter ligation to the break-sites. DNA was then purified and fragmented. In vitro transcription was used to generate single-stranded RNA-fragments from the adapter-ligated DNA. A second adapter-ligation was then followed by reverse-transcription. Single-stranded cDNA was used as template for a PCR that also introduced Illumina sequencing-adapter sites and sample-specific indexes. Overall, libraries were prepared for sequencing using a modified NEBNext Small RNA library preperation protocol (following the main steps outlined in the paper "Yan et al., 2017, Nature Communications").