GSM8290925: BMDCs, LMF, C13; Mus musculus; RNA-Seq
RNA was harvasted using Trizol, and using a NanoDrop 2000 and an Agilent 2100/4200 system, the purity and concentration of total RNA were quantified. For RNA sequencing, mRNA was purified using polyT selection. After the QC procedure, the RNA with poly-A in eukaryotic total RNA was enriched by TIANSeq mRNA Capture Kit (TIANGEN). Then, using the captured RNA as the starting sample, TIANSeq Fast RNA Library Kit (Illumina) was used to construct the transcriptome sequencing libraries.