GSM7696765: VDR ATAC siNfia 3; Mus musculus; ATAC-seq
To isolate GNP and hyperplasia cells from P7 and P28 Atoh1EGFP cerebella, the tissues were dissociated, followed by percoll gradient centrifugation as previously described. For tumor cell isolation, tumor-bearing cerebella was dissociated with Trypsin/DNase and filtrated though 40 μm cell strainer (CORNING). Subsequently, the cells were suspended in cold DPBS containing 1% BSA for cell sorting of EGFP positive cells with FACSAriaTM sorters (BD Biosciences). Libraries were prepared with the Omni-ATAC protocol.