GSM6532831: CGN, DMSO, H3K27me3, CUT&RUN, Rep 1 [CGN_DMSO_biol_rep_1]; Mus musculus; OTHER
Cells were scraped into 1X PBS + Protease Inhibitor, pelleted and washed in the same buffer, after which intact nuclei were pelleted and CUT and RUN was performed according to the Epicypher CUT and RUN kit (#14-1408) as per manufacturer guidelines CUT and RUN libraries were prepared using the NEB Ultra II DNA Library Prep Kit for Illumina (NEB #R7645L) and NEBNext Multiplex Oligos for Illumina (NEB #E6440S). Library Cleanup was performed prior to and after PCR amplification (as noted in Epicypher CUT and RUN Kit, #14-1408), using 0.8X Kapa Hyperpure beads (Roche #08963851001)