GSM7857166: Sample3: AA025_R1; Homo sapiens; RNA-Seq
Sample tissues were disgregated and digested for cellular recovery. Single cells were captured in gel bead-in-emulsions. Single cells were encapsulated with fixed Illumina Read 1 sequence, a cell-identifying 16 bp 10x Genomics barcode, a 12 bp Unique Molecular Identifier (UMI) and a poly-dT sequence. Upon cell lysis, reverse transcription yielded full-length, barcoded cDNA that was PCR-amplified. Fragmented cDNA was end-repaired, A-tailed and ligated to Illumina adaptors. A final PCR-amplification with barcoded primers allowed sample indexing.