GSM7993675: Kidney organoids differentiated in AggreWell; Homo sapiens; RNA-Seq
Kidney organoids were collected from both the AggreWell and UniMat using a pipette, and then dissociated into single cells using Accutase at 37 °C for 20 min. The single cells were resuspended in the basal differentiation medium containing 1.5% FBS. samples were prepared following the manufacturer's protocol (10X Genomics). The single-cell libraries were sequenced using the Chromium Next GEM Single Cell 3' RNA library and gel bead v3.1 kit in HiSeqXten (Illumina).