GSM7688451: Rbpj_cko_scRNA; Mus musculus; RNA-Seq
Mouse brains were dissected out and immediately submerged in fresh ice-cold Hanks balanced salt solution (Gibco 12175-095). The cortex was then cut into pieces and dissociated into a single-cell suspension using a Papain Cell Dissociation Kit (Miltenyi Biotec, catalog no. 130-092-628) according to the manufacturer's instructions. CellTrace Yellow labeled single-cells were sorted using a BD FACSAriaII. The Chromium droplet-based sequencing platform (10× Genomics) was employed to generate scRNA-Seq libraries, following the manufacturer's instructions (manual document part number: CG00052 Rev C).