GSM8032531: LPM_WT Ecoli 1; Mus musculus; RNA-Seq
Mice were subjected to intraperitoneal infection for 30 minutes and underwent peritoneal lavage to acquire peritoneal cells. Cells were washed with ice-cold PBS and collected in FACS buffer. Cells further underwent surface staining for LPM sorting. Subsequently, the Sony MA900 Multi-Application Cell Sorter was used for isolating LPM cells. 3 samples from PBS- and infection-treated mice from control or Cx3cr1-PAC−/− mice were collected. Total RNA was extracted using the RNeasy Micro kit (Qiagen). Total RNA quality was determined by the A260/A280 and A260/A230 as well as the Agilent 2100 Bioanalyzer. NEBNext Ultra II RNA Library Prep Kit