GSM7917297: NCV_0927; Mus musculus; RNA-Seq
For the quality check and counting of single cell suspension, the cell survival rate is generally above 90%. The cells that have passed the test are washed and resuspended to prepare a suitable cell concentration of 700~1200 cells/μl. Then the cells were loaded ~18,000 cells/chip position using the 10× Chromium Next GEM Single Cell 5' Kit v2. The system is operated on the machine. GEMs (Gel Bead in Emulsion) were constructed for single cell separation according to the number of cells to be harvested.