GSM7518458: Skin, excised, cultured, expanded, for H3K9me3 ChIP; Homo sapiens; ChIP-Seq
Cells were fixed in 1% formaldehyde, lysed in 50 mM Tris-HCl, pH 8, 10 mM EDTA, 1% SDS, protease inhibitors and Na-butyrate, and sonicated into ~200 bp fragments using a Picorupter (Diagenode). After sedimentation,  chromatin was incubated with anti-lamin A/C antibodies at 10 µg/5x10e6 cells or with anti-H3K9me3 antibodies at 2.5 µg/10e6 cells. Cross-links were reversed, DNA purified, eluted in H2= and processed for library preparation. Libraries were prepared using the Microplex kit (Diagenode)