GSM7109891: NC_2; Homo sapiens; RNA-Seq
Total RNA was treated with RQ1 DNase (Promega) to remove DNA. The quality and quantity of the purified RNA were determined by measuring the absorbance at 260nm/280nm (A260/A280) using Nanodrop One (Thermo). RNA integrity was further verified by 1.5% agarose gel electrophoresis. For high-throughput sequencing, the libraries were applied to Illumina Novaseq 6000 system for  150 nt paired-end sequencing.