GSM7036238: Longissimus, D 243 postnatal, rep 3; Ovis aries; RNA-Seq
Total RNA was extracted with Trizol according to manufactures specifications. Qantificatioon and qualification were checked by 1% agarose gel, NanoPhotometer spectrophotometer, and Agilent Bioanalyzer 2100 system. NEBNext Poly(A) Magnetic Isolation Module was used to purify, fragment, and add random primers to mRNA according to the manufacturer. MEBNext Ultra II RNA Library Prep Kit for Illumina was used according to the manufacturer for library preparation.