GSM7073634: gCd8 Th1 rep3; Mus musculus; RNA-Seq
Cells were directly sorted into 1.7 mL microtubes containing 500 μL Trizol LS buffer. RNA extraction was performed using miRNeasy micro kits for downstream RNA-SmartSeq2 input requirements (Rosales et al. 2013). cDNA was purified using AMPure XP beads. O0.5 nanogram cDNA was used to prepare a standard Nextera XT sequencing library (Illumina Nextera XT DNA sample preparation kit and index kit). Samples were sequenced using a HiSeq2500 (Illumina) with single-end sequencing.