GSM6754762: BAL_1; Mus musculus; RNA-Seq
Cell concentration and viability of mouse samples were measured using the Countess II instrument and ranged from 8 – 9 x 105 cells/mL with ~90% viability (Thermofisher Scientific, USA).  Cell samples were input into 10x Genomics Single Cell 3′ v3 workflow and processed according to the manufacturer's instructions for a targeted recovery of 5000 cells (10X Genomics, USA).  Resulting cDNA libraries were sequenced on an Illumina NextSeq 500 instrument with 1.8pM input and 1% PhiX control library spike-in (Illumina, USA). scRNA-seq libraries were generated in BU DOM Single Cell sequencing core facility and sequenced by BU Microarray and Sequencing Resource Core Facility according to 10x Genomics protocol