GSM6530665: Female, scRNAseq; Mus musculus; RNA-Seq
For the normal situations, the omentum from 6 male/female mice were pooled for single cell isolation. For the activation stats, the omentum from 2-3 male/female mice were pooled for single cell isolation. Briefly, omemum were chopped into small pieces and digested in collagenase I (Sigma, C0130) for 10 min, 20min, 30min at 37 °C. Cells were collected at  the different time points in order to get all kinds of cells from the omentum. Then the cells were washed in serum-free medium and then passed through a 40 um cell strainer to generate a single cell suspension. Single cells around 10,000 -15,000 cells from naive and activated omentum were used to generate single cell RNA-seq libraries which was further sequenced and analyzed. Single cells per sample were encapsulated in droplets using 10x Genomics Gemcode Technology and processed following the manufacture's protocols (#CG00052). cDNA and libraries were checked for quality on Agilent 4200 Tapestation and quantified by KAPA qPCR before sequencing on a single lane of a HiSeq4000 (Illumina).