Characterisation of the in-vivo miRNA landscape in Drosophila ribonuclease mutants reveals Pacman mediated regulation of the highly conserved let-7 cluster during apoptotic processes
Characterisation of the in-vivo miRNA landscape in Drosophila ribonuclease mutants reveals Pacman mediated regulation of the highly conserved let-7 cluster during apoptotic processes
Wing imaginal discs were dissected from wandering 120hr old L3 larvae in ringers solution, snap frozen and stored at -20 Larvae were cultured on standard media using a 3hr egg lay RNA was extracted from dissected wing imaginal discs using the Qiagen miRNeasy Micro Kit RNA was depleted for the 2S rRNA and small RNA seq libraries were prepared using the QIAseqÂ® miRNA Library Preparation Kit