GSM7994779: CD45_plus__19-018; Homo sapiens; RNA-Seq
The tissue dissociation was performed using Multi Tissue Dissociation kit 1 (Miltenyi Biotec, 130-110-201) as per manufacturer's instructions with slight modifications. Single Cell 3' v3 from 10X Genomics (sequencing protocol)  Libraries were sequenced at 60,000 fragment reads per cell following Illumina's standard protocol using the Illumina cBot and HiSeq 3000/4000 PE Cluster Kit. The flow cells were sequenced as 100 X 2 paired end reads on an Illumina HiSeq 4000 using HiSeq 3000/4000 sequencing kit and HCS v3.3.52 collection software. Base-calling was performed using Illumina's RTA version 2.7.3.