GSM7230924: HCC-TAMs, shSPP1 2; Homo sapiens; RNA-Seq
Total RNA was used as input material for the RNA sample preparations. Sequencing  libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA,  Catalog #: E7530L) following manufacturer's recommendations and index codes were  added to attribute sequences to each sample. The qualified libraries were pooled and sequenced on Illumina platforms with PE150  strategy in Novogene Bioinformatics Technology Co., Ltd (Beijing, China), according to  effective library concentration and data amount required.