GSM6435279: 48_3P; Homo sapiens; RNA-Seq
Surgically resected brain tissue was dissociated in a glass dounce homogenizer and then nuclei were washed and stained with Hoechst dye, as previously described [Koboldt et al., 2021]. Approximately 10,000 Hoechst-positive nuclei per sample were sorted on a BD Influx cell sorter directly into master mix from the 10x Genomics Next GEM Single-Cell 3' or 5' reagent kits. 10x Genomics Chromium Single Cell 3' v3.1 single-index kit was used according to the manufacturer's protocol. paired-end sequencing using the following read configuration: 28 (R1), 8 (i7), 0 (i5), 91 (R2) (3'v3.1) or paired-end sequencing using the following read configuration: 28 (R1), 8 (i7), 0 (i5), 90 (R2) (5'v2)