GSM6482154: P6 Fucci Retinal Endothelial Cells - Green 1 [REC_SG2M-1]; Mus musculus; RNA-Seq
QIAGEN RNeasy MinElute Kit was used to purify RNA. Low cell isolation numbers required cDNA amplification for RNA sequencing. RNA preparation and cDNA amplification for Illumina next-generation sequencing was performed with the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Bio USA, Inc.). Retinal endothelial cell samples were transferred to the University of Virginia Genome Analysis and Technology Core, which performed cDNA library preparation with the DNA Prep, (M) Tagmentation kit (Illumina Cat #20018704). HUVEC-FUCCI samples were transferred to the Yale Center for Genomic Analysis, which performed cDNA library preparation and amplification.