GSM8031790: Control CD45; Mus musculus; RNA-Seq
At the endpoint, mice were euthanized humanely, tumors were collected, parts of the MOC2 tumors were processed for scRNA-seq, and the remaining tumors were snap-frozen in liquid nitrogen for further analysis. Tumors (3 mice per group-pooled) were processed for single-cell isolation followed by cell sorting using FITC-conjugated CD45 antibody and propidium iodide (PI) staining, as described previously (22), with modifications. Libraries were generating as directed by the manufacturer using the 10X Genomics Chromium™ Next GEM Single Cell 3' Kit v3.1.