GSM7518911: RELA_rep1; Homo sapiens; RNA-Seq
Total RNA extraction from IDH-wt-hGICs-MGT#4-dEGFP dCas9-VPR cells was performed using the TRIzol Reagent (Invitrogen, 15596026), followed by isopropanol precipitation and subsequent AMPure XP beads (Beckman Coulter, #A63881) purification. Multiplexed 3'-cDNA libraries were constructed starting from 60 ng of total RNA per sample as input, utilizing barcoded oligo-dT primers (Datlinger et al., 2021) in an adapted version of Bulk RNA barcoding and sequencing (BRB-seq) protocol (Alpern et al., 2019).