GSM6857612: ATAC_Neg2-ctrl high 1; Mus musculus; ATAC-seq
Organoids were isolated and dissociated to single cells and 50,000 live cells were sorted in 200 mL 0.1% BSA/PBS for generation of ATAC-seq libraries. Briefly, sorted cells were lysed and incubated with Tn5 Transposase (Illumina) for 30 minutes at 37C for DNA tagmentation The tagged DNA was PCR amplified with sequence-compatible and barcoded primers for a total of 13 cycles. The concentration and quality of the libraries was analysed with Qubit and Bioanalyzer. The libraries were diluted to 2 nM in EB buffer and a final dilution of 1.5 pM was sequenced with Illumina High Output 150 pair-end  cycles kit on an Illumina NextSeq 500 sequencer.