GSM7073135: shCTRL DMSO r3; Homo sapiens; RNA-Seq
RNA was extracted using the total RNA purification kit by Norgen Biotech Corp, following the manufacturer's guidelines. Quantification of total RNA samples was performed with a Qubit HS RNA assay (ThermoFisher Scientific), and fragment size was evaluated with a Fragment Analyzer HS NGS assay (AATI). RNA quality numbers (RQNs) of 8.0 (or higher) were considered satisfactory for library construction. Library construction was performed with a Truseq RNA v2 (Illumina). Libraries were prepared with unique barcodes compatible with the Illumina NextSeq 500 platform. Quantification of the libraries was performed with a Qubit HS DNA assay, and library fragment size was evaluated with a Fragment Analyzer HS NGS assay. Libraries were normalized to the same concentration, and then samples were pooled in equal amounts.