Replicate 2, starting point: 0 hours after start of induction; no induction (i.e., mock induction with 0.0 microM estradiol)
RNA was purified using Invitrogen Dynabeads oligo (dT)25 (Cat# 61002). Purified RNA was fragmented at 94 deg. C. and first-strand cDNA synthesis was performed using Invitrogen random hexamer (Cat# 48190011) and Invitrogen Superscript II (Cat# 18064014) followed by second-strand synthesis using Fermentas DNA Pol I (Cat# EP0041).