GSM8254255: DLD-1 cells, AID, ctrl, CTCF, rep1; Homo sapiens; OTHER
CUT&Tag libraries were prepared per a previously established protocol 5 (https://www.protocols.io/view/bench-top-cut-amp-tag-kqdg34qdpl25/v2?version_warning=no). Briefly, 0.25M cells were used for each reaction. Two replicates were performed for each experiment. Primary antibodies used were: GATA6(CST, 5851), CTCF (ABclonal, A18627), H3K27ac (active motif, 39133), RAD21 (ABclonal, A18850). CUT&Tag library was contrucedd following a previously pulished protocol (Kaya-Okur et al. Nature Communication 2019)