RNA-Seq of leaf: sapling of Castanopsis fissa
Oligo dT magnetic beads were used to enrich mRNA, then fragment, then reverse transcribed the first and second strands of cDNA, and then the whole library was prepared by terminal repair, A-tail addition, sequencing joint addition, purification, PCR amplification and other steps. After qualified library detection, different libraries are pooling into Flow cells according to the requirements of effective concentration and target data volume. After cBOT clustering, Illumina NovaSeq, Illumina's high-throughput sequencing platform, is used for sequencing