Ion Torrent PGM sequencing; Functional Annotation of the Bovine Genome
Functional Annotation of the Bovine Genome
The set of reference tissues included a total of 40 tissues and 13 assays from two adult L1 cattle. Furthermore, samples from L1 fetuses/placentas (7 fetal tissues, 2 placental tissues, and 2 maternal tissues) were also collected. Tissues/cells were collected, snap-frozen in liquid nitrogen and stored at -80Â°C for the purpose of DNA and RNA extraction, ATAC-seq, and ChIP-seq processing. Please see:  Zhou X, Li R, Michal JJ, Wu XL, Liu Z, Zhao H, Xia Y, Du W, Wildung MR, Pouchnik DJ, Harland RM, Jiang Z. Accurate Profiling of Gene Expression and Alternative Polyadenylation with Whole Transcriptome Termini Site Sequencing (WTTS-Seq). Genetics. 2016 Jun;203(2):683-97.