GSM6529635: HBV-POS1-LOD6-20200924; Hepatitis B virus; RNA-Seq
A pair of primers (forward primer sequence: ccggaaagcttgagctcttcTTTTTCACCTCTGCCTAATCA; reverse primer sequence: ccggaaagcttgagctcttcAAAAAGTTGCATGGTGCTGG) were designed based on the open loop region for long fragment amplification to cover the whole length of HBV DNA with PCR buffer of 2 × Vazyme LAmp® Master Mix (Vazyme Biotech Co. Ltd, Nanjing, China). After purification of the PCR product, long fragment HBV DNA were performed the random fragmentation via ultrasound fragmentation instrument of LE220 (Covaris, Woburn, Massachusetts) and utilized the NEXTflex Rapid DNA-Seq Kit for Illumina (Bioo Scientific, Austin, Texas) to obtain the DNA libraries. Quality of long fragment amplification products were assessed by gel electrophoresis while quality of amplified libraries was assessed by capillary electrophoresis with a DNA chip on a 2100 Bioanalyzer