GSM7696368: Cardiac fibroblast, TAC, 4 week, rep 2; Mus musculus; ncRNA-Seq
TRI Reagent (Molecular Research Center) was used to isolate total RNA in accordance with the manufacturer's protocol. DNase I (Takara) was used to remove residual DNA in RNA samples. Total RNA was treated with Ribo-Zero Gold rRNA Removal Kit (Illumina) to remove the ribosomal RNAs and applied to TruSeq Stranded Total RNA Kit (Illumina) to construct RNA-seq library. The library was sequenced by HiSeq 2500 (Illumina) in the paired-end mode with 100 sequencing cycles.