GSM7157620: 5CC7 + wildtype TNFa-pos scRNA; Mus musculus; RNA-Seq
NA Fifteen-thousand sorted cytokine-positive CD8+ T cells (CD3+CD8+IL-2+ or CD3+CD8+TNFα+) were separately partitioned into droplets for single-cell omics assays via Chromium Next GEM Single-Cell 5'Kit v2 (10x Genomics, 1000263). RNA-seq libraries were prepared according to manufacturer protocols. TCR-seq libraries were prepared via the Chromium Single Cell Mouse TCR Amplification Kit (10x Genomics, 1000254). All sequencing libraries (RNA-seq, TCR-seq) were quantified via the Qubit dsDNA HS Assay Kit (Invitrogen, Q32851), quality-checked for fragment sizes via high-sensitivity D5000 ScreenTapes (Agilent, 5067-5592), pooled, and sequenced (Illumina, Novaseq-6000). paired scRNA-seq and scTCR-seq