GSM6858332: spike-specific B cells ,B cells, D15,scRNAseq,VDJ; Homo sapiens; RNA-Seq
Live CD19+ IgG+ spike-FusionRed-binding memory B cells were sorted on a MoFlo Astrios Cell Sorter. FusionRed-negative CD19+  IgG+ B cells were sorted in parallel and mixed with spike sorted B cells. FACS staining  solution also contained barcoded hashtags for sample identifications (BioLegend) as well as barcoded RBD. Libraries for single-cell transcriptome sequencing and scBCR sequencing were prepared using the, Chromium Single-Cell 5′ Library Gel Bead and Construction Kit, v2 Dual index Chemistry Kit and Chromium Single-Cell V(D)J BCR Enrichment Kit (10x Genomics, CA, USA).