mRNA-Seq of human mitochondrial myopathy patients: muscle 12 weeks
Total RNA was extracted from muscle samples using the Qiagen miRNeasy kit according to manufacturers instructions. RNA integrity and quantity was assessed on an Agilent Tapestation system (minimum requirements: RIN >7, >2000ng RNA and [RNA] >50 ng/l). mRNA libraries were created using the Illumina TruSeq Stranded mRNA kit and sequenced on the Illumina NextSeq 500 platform following the manufacturers protocols