GSM7518288: L131_Sepsis_ADT; Homo sapiens; OTHER
PBMC were extracted from whole neonatal blood and frozen at -80C. Paired samples from the same baby were thawed on the day of the experiment and incubated with Totalseq antibodies or hashtag reagents prior to sorting of live PBMC (4/5 babies) or CD3+ T cells (1/5 babies). Cells were loaded onto a 10x Chromium Chip A using reagents from the Chromium Single-Cell 3' gene expression and antibody derived tag (ADT) libraries were prepared according to the manufacturer's instructions. Samples were sequenced with the Illumina NextSeq 500 platform, or Hiseq 2500 High Output platform dependent on the experiment. For libraries names beginning 'EL_Pre' or 'EL_Sepsis'  sorted cells were loaded onto a 10x Genomics Chromium controller and 5' gene expression and ADT libraries were prepared.