GSM7109965: LepR+ PMMA Fibrotic [Sample_LepR.plus.B_L002]; Mus musculus; RNA-Seq
LEPR+ cells obtained from peri-implant bone or fibrous tissue from mouse undergoing tibia implantation. Single cells were obtained, stained for Flow cytometry antibodies and FACS was conducted to sort the desired cell types. RNA sample were prepared using the RNAeasy Plust Micro Kit Cat no 74034. cDNA libraries were generated using the Illumina TruSeq RNA Sample Preparation kit and sequenced on NovaSeq 6000 sequencer