GSM6921580: pilot_par_organoid_4; Homo sapiens; RNA-Seq
RNA was extracted using TRIzol reagent (ThermoFisher) according to manufacturers instructions. RNA samples were cleared of contaminating genomic DNA by DNAse I (Roche) treatment for 1h at 37C. RNA cleanup and DNAse I removal was performed using RNeasy MinElute columns (Qiagen) according to manufacturer's instructions. Clean RNA was assessed for quality on an Advanced Analytical Fragment Analyzer. All samples had an RQN > 7.5 and strand-specific sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina in conjunction with the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Multiplex Oligos for Illumina (New England Biolabs).