GSM6754168: Rbbp4-AID 0h_H3K9me2_rep2; Mus musculus; OTHER
CUT&Tag assay was done as described in Kaya-Okur et al (2019) with slight modifications. 1 × 105 cells were harvested and bound to Concanavalin A-coated beads, then incubated with individual primary antibody at 4°C overnight. pG-Tn5 (Vazyme, S602) enzyme was added to each sample followed by incubation 1 hour at RT. pG-Tn5 was actived by 10 mM MgCl2 at 37℃ for 1 hour. The DNA released into the supernatant were purified and subjected to library preparation and next-generation sequencing. The sequencing libraries were prepared using NEBNext High-Fidelity 2x PCR Master Mix (NEB, M0541S) for 13 cycles.