Genotyping by sequencing of VCC13
DNA samples were normalised to 200 ng suspended in 10 L water and sent to the Genomic Analysis Platform, Institute of Integrative and Systems Biology, Universite Laval (Quebec, Canada) for GbS library preparation. DNA was digested with a combination of restriction enzymes PstI/NsiI/MspI, ligated with barcoded adapter, and pooled to equimolarity. The pooled library was amplified by PCR and sequenced on an Illumina NovaSeq6000 S4 with paired-end reads of 150 bp at the Genome Quebec Innovation Centre (Montreal, Canada).