GSM7109875: 7D,smartseq2,dKO,rep2; Mus musculus; RNA-Seq
We used WT and Prmt1 conditional knockout samples for all the experiments through tamoxifen injection for 3 days. For WT CUT&Tag we used C57BL/6J; single cell were were isolated from testis. Further proceeded for library preparation using KIT manual. For RNA-seq we isolated total RNA from testis and for smart-seq2 we used isolted spermatogonia for downstream procedure and library preparation. CUT&Tag libraries were prepared using Hieff NGS® G-Type In-Situ DNA Binding Profiling Library Prep Kit (Yeasen)