GSM8330353: MDA-MB-231, parental, LSD1, CST, rep2; Homo sapiens; OTHER
Cells were binded to Concanavalin A coated beads, permeabilized and incubated with anti-LSD1 antibody or Normal Rabbit IgG. pAG-Mnase binded to antibodies and digested targeted chromatin. CUT&RUN libraries were prepared using the NEBNext UltraII DNA Library Prep Kit (NEB). 10 ng of DNA was used as starting material for input and ip samples. Libraries were amplified 11 cycles on the thermocycler. Post amplification libraries were gel-purified for size selection at 300-500bp. Libraries were validated using Tapestation (Agilent) and quantified using Qubit Fluorometer (Invitrogen).