GSM7036529: macrophage, mfap4 positive/tnfa negative, non-infected, 4 days, rep 1; Danio rerio; RNA-Seq
Macrophages were sorted by FACS and RNA was harvested using Rneasy mini plus kit (Qiagen). 5 ng of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using kit SMART-Seq v4 Ultra Low Input RNA Kit from Takara and the kit DNA Prep from Illumina following manufacturer's protocols.