GSM7996124: Ovary, LR, S15; Homo sapiens; miRNA-Seq
Small RNAs were extrated from an average of 10 sections per sample using the miRNeasy FFPE kit (Qiagen). Quality tests, including qRT-PCR and gel electrophoresis, were used to confirm successful miRNA isolation without contamination. Libraries were prepared using the TruSeq Small RNA library Prep Kit (Illumina, San Diego, CA), validated on the Agilent TapeStation 4200 (Agilent Technologies, Palo Alto, CA, USA), and quantified using a Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA).