GSM8290614: E13.5 Spinal cord, NFIA, ChIP; Mus musculus; ChIP-Seq
Spinal cords were homogenized and fixed with 1% formaldehyde for 15 min. After quenching  and lysis, chromatin was sonicated and the DNA sheared to an average length of 300-500 bp using Active Motif's EpiShear probe sonicator (cat# 53051). Genomic DNA (Input) was prepared by SPRI beads clean up (Beckman Coulter) and quantitation by Clariostar (BMG Labtech). 40 μg of DNA was used as a starting material for input and IP. Libraries were prepared using an automated system (Apollo 42, Wafergen Biosystems/Takara) and sequenced  on  Illumina NovaSeq 6000 sampes. Immunoprecipitation was performed with 30 μL of anti NFIA antibody (Atlas Antibodies, #HPA008884).