RNAseq of Lotus japonicus WT nodule nitrate 24h replicate 2
mRNA was randomly sheared in buffer and cDNA is synthesized by using mRNA template and barcoded oligodT primer, after which second-strand synthesis buffer (Illumina) , dNTPs, RNase H and DNA polymerase I are added to initiate the second-strand synthesis. After terminal repair, and sequencing adaptor ligation, the double-stranded cDNA library is completed through size selection and PCR enrichment.