GSM6481349: Monocytes, mRNA-derived cDNA; Homo sapiens; RNA-Seq
Peripheral Blood Mononuclear Cells (PBMC) were prepared by centrifugation on a Ficoll gradient (Lymphoprep, StemCell). Blood CD14+ monocytes were isolated from healthy donors' PBMC by positive selection using magnetic beads (Miltenyi). Monocytes were 95%–98% CD14+CD16- as assessed by flow cytometry. Library was prepared using Chromium Single Cell 3ʹ v3.1 protocol (10x Genomics).