H2
A ABclonal mRNA-seq Lib Prep Kit (ABclonal, China) was used to prepare paired-end libraries according to the manufacturer's instructions. Subsequently, first-strand cDNAs were synthesized with random hexamer primer and Reverse Transcriptase (RNase H) using mRNA fragments as templates, and the second-strand cDNA was synthesized using DNA polymerase I, RNAseH, buffer, and dNTPs. Then, the synthesized double stranded cDNA fragments were adapter-ligated to prepare the paired-end library. PCR products were purified (AMPure XP system) and evaluate the library quality on an Agilent Bioanalyzer 4150 system. The raw reads in fastq format were processed through in-house perl scripts to generate clean reads, and then the HISAT2 software was used to align them separately with the reference genome in a directed pattern to obtain mapped reads.