GSM7073001: Hippocampus, Trap1WT, male, sample3; Mus musculus; RNA-Seq
RNA was extracted from the mouse hipocampii using TRIzol (Thermo Fisher Scientific), DNA contamination was removed by 2 U of TURBO DNase (AM2238, Ambion) in the supplied buffer in 37°C for 30 min. Next, RNA was re-isolated with phenol/chloroform, precipitated with ethanol, and resuspended in 50 μl of RNase free water. Total RNA libraries were prepared using TruSeq Stranded Total RNA Library Prep (Illumina, nr kat. 20020596) and adapters from Illumina TruSeq RNA UD Indexes (96 Indexes, 96 Samples) (Illumina, nr kat. 20022371), following manufacturer's protocols.