Illumina NovaSeq 6000 paired end sequencing; scRNA-seq data from GLI3 KO in brain organoids
scRNA-seq data from GLI3 KO in brain organoids
Single cell suspensions were acquired by dissociation of the organoids with a papain-based dissociation (Miltenyi Biotec, 130-092-628).  Cerebral organoids were generated using a whole brain organoid differentiation protocol (Lancaster et al. 2013). Single cell RNAseq libraries were generated with the Chromium Single Cell 3' V3 Library & Gel Bead Kit. Single cell encapsulation and library preparation were performed according to the manufacturer's protocol.   Single cell RNAseq libraries were generated with the Chromium Single Cell 3' V3 Library & Gel Bead Kit. Single cell encapsulation and library preparation were performed according to the manufacturer's protocol.