PacBio_Reads
Concentrated virus was purified through a 10% sucrose cushion, extracted using a genomic tip 20/G (Qiagen), quantified using a Qubit fluorometer (Thermo Fisher Scientific), libraries prepared using the SMRTbell Express Template Prep kit 2.0 (Pacific BioSciences), DNA sheared using the Megaruptor 3 (Diagenode) and size selected using Ampure PB beads (Pacific BioSciences) for sequencing on the Sequel IIe system (Pacific BioSciences) using the Sequel II binding kit 2.2 and sequencing plate V2 (Pacific BioSciences)