Talaromyces sp.genome sequencing and assembly
High quality genomic DNA (gDNA) was isolated from the received culture using QIAamp DNA mini kit. gDNA that passed the preliminary agarose gel inspection was further subjected to purity check spectrophotometrically to measure the A260/280 ratio and its concentration was measured using Qubit broad range dsDNA quantification kit (Thermo Fisher Scientific). 250ng of total DNA was used as input for library preparation using QIASeq FX DNA kit to fragment and obtain adapter ligated and indexed library as per manufacturers instructions. The indexed library was sequenced on an Illumina Miseq using a 300 cycle paired-end chemistry. Analysis steps that were performed on the sequence data are represented in Figure 2. In essence, all reads are checked for quality and then QCed reads are passed through the process flows simultaneously.