GSM7035856: MOLM-13 shDDX6 rep2; Homo sapiens; Hi-C
After fixating the cells, cell lysis and isolation of nuclei, chromatin was restriction-digested, followed by Klenow fill-in and ligation of DNA fragments.DNA ligation products were sheared by sonication, repaired, and immunoprecipitated. DNA fragments were ligated to adapters, and the products were amplified by PCR.The amplified library was size-selected, enriched for promoter-containing ligation products, and amplied by PCR.