RNA-seq of Corynebacterium glutamicum wild-type/pXMJ19
To compare MprA expression from the plasmid in the mprAB mutant strain and the empty plasmid in the mprAB mutant strain, 1 mM IPTG was added to the medium at OD600=5.0-6.0. The cultures were harvested after one hour. Cells were washed three times using PBS buffer (137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO4, 1.4 mM KH2PO4), harvested by centrifugation at 4C, and the pelleted cells were quick-frozen in liquid nitrogen. RNA-Seq was performed by Novogene (Beijing, China). The comparisons were performed using three biological replicates.