GSM6529487: M82 root, replicate 1, scRNAseq; Solanum lycopersicum; RNA-Seq
Single cell libraries were then prepared by the UC Davis DNA Technologies Core. The protoplast suspension was then loaded onto microfluidic chips (10X Genomics) with v3 chemistry to capture 10,000 cells/sample. Cells were barcoded with a Chromium Controller (10X Genomics). mRNA was reverse transcribed and Illumina libraries were constructed for sequencing with reagents from a 3' Gene Expression v3 kit (10X Genomics) according to the manufacturer's instructions. Sequencing was performed with a NovaSeq 6000 instrument (Illumina) to produce 100bp paired end reads.