GSM7110074: Donor3688-D7_Control; Homo sapiens; ATAC-seq
Donor peripheral blood NK cells were isolated from a LRS chamber and CRISPR-edited by electroporation with Cas9 mRNA and gRNA targeting EOMES and TBX21. Control NK cells were electroporated without gRNA. CRISPR-edited cells were either maintained in vitro in 1ng/mL IL-15. On Day 7 and Day 10 post CRISPR electroporation, NK cells were harvested from tissue culture plate for library preparation Nuclei and libraries were prepared for ATAC sequencing following established protocol as reported in https://www.nature.com/articles/nmeth.2688. Nuclei were isolated and Illumina Tagment DNA TDE1 Enzyme and Buffer Kit was used to perform the tranposition reaction. PCR Amplification was performmed using the NEB kit with Illumina indexing primers. A qPCR reaction on the side was performed to identify the approciate number of PCR amplication cycles to perform so that amplification is stopped prior to saturation