GSM6858798: 10x_Zou_1_Heart; Mus musculus; RNA-Seq
The adoptively transferred TEa cells were stained with fluorochrome-conjugated antibodies, and sorted from the spleens and heart allografts of the recipients by a cell sorter Single cell Gene Expression Library was prepared according to Chromium Next GEM Single Cell 3' Reagent Kits v3 (10x Genomics). In Brief, single nuclei suspension, reverse transcription (RT) reagents, Gel Beads containing barcoded oligonucleotides, and oil were loaded on a Chromium controller (10x Genomics) to generate single cell GEMS (Gel Beads-In-Emulsions) where full length cDNA was synthesized and barcoded for each single cell. Subsequently the GEMS are broken and cDNA from each single cell are pooled. Following cleanup using Dynabeads MyOne Silane Beads, cDNA is amplified by PCR.  The amplified product is fragmented to optimal size before end-repair, A-tailing, and adaptor ligation. Final library was generated by amplification. 