GSM7886368: cortex_P10_wt; Mus musculus; RNA-Seq
Nuclei from whole cortex were obtained from one mouse at age P10, anesthetized with isoflurane and sacrificed by decapitation. The dissected cortex tissue was immediately placed in a dry-ice-cold tube for immediate freezing until processing for nuclei isolation. Dissected frozen cortex was resuspended and mechanically homogenized using dounce homogeneizer to release nuclei following the Salty EZ 10 protocol (dx.doi.org/10.17504/protocols.io.bx64prgw). Nuclei were then resuspended in 500μL of resuspension buffer and 50k  nuclei were sorted (BD ARIA) based on DAPI intensity. Nuclei were finally centrifuged at 500xg for 5 minutes and diluted in resuspension buffer to a concentration of 1200 nuclei/μl before encapsulation in 10x Chromium. All steps were carried on ice or at 4°C. Libraries were prepared according to 10x Genomics 3' version 3.1 chemistry & Illumina protocols