GSM6529508: lung, normal mouse, challenged by Methacholine for 30 days, biol rep4; Mus musculus; RNA-Seq
Total RNA was extracted using RNeasy Kits (Qiagen, Duesseldorf, Germany) and treated with DNase I (Life Technologies, Gaithersburg, USA) according to standard protocols. Sequencing libraries were constructed using the TruSeq Stranded Total RNA kits (Genesky, Shanghai, China)