Demultiplexed maize leaf sci-ATAC-seq replicate 2
Librarieswerepreparedusing24Tn5adapters:ME-Aand16ME-B,eachcontaininga5ntcellbarcode.ME-Asequence:GGCGGTAGGCGTGNNNNNAGATGTGTATAAGAGACAGME-Bsequence:CCAACACCCGTGCGNNNNNAGATGTGTATAAGAGACAGThe24columnbarcodesusedwere:ATACG,AGCGA,CTAAG,TGTCG,GTAGT,GTATA,ATTCC,GACTT,TTACT,CAATT,CATCT,TCGCG,TGTTT,TCCCT,GGCAG,CAAAA,GTGAG,TGGAT,CCGAG,ACTCG,GTCCT,TAAGA,ACCCC,AAACA.The16rowbarcodesusedwere:TCGGA,GTTTC,AGCTT,TCATT,GCTCC,ACTAA,CGAGG,ACCGG,CGGTT,GGAAC,GATGA,AACAT,CGCAC,TGACC,CGCGT,TTCCC.AfterTn5tagmentation,approximately10nucleiweredistributedtoeachwellofa96-wellplate.PCRwasperformedusing8well-indexedforwardprimers(ScPCR_F)and12well-indexedreverseprimers(scPCR_R),eachcontaining6ntbarcodes.ScPCR_F:ACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNNNNGGCGGTAGGCGTGScPCR_R:GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTNNNNNNCCAACACCCGTGCGThe8forwardprimerbarcodesusedwere:GATCAG,TAGCTT,GGCTAC,CTTGTA,AGTCAA,AGTTCC,ATGTCA,CCGTCC.The12reverseprimerbarcodesusedwere:GTAGAG,GTCCGC,GTGAAA,GTGGCC,GTTTCG,CGTACG,GAGTGG,GGTAGC,ACTGAT,ATGAGC,ATTCCT,CAAAAG.Aftersequencing,thedatafromeachplatewasdemultiplexedusingthe24x16celland8x12wellbarcodes.Additionally,a6ntplatebarcodewasaddedtothecell+wellbarcodebeforecombiningthedatafromeachplate.