GSM7917854: RIP_SNRP70_01; Homo sapiens; RIP-Seq
For each RIP, 1.2x10^6 of replicative senescent ECFCs were used. Immunoprecipitation was performed with anti-RIG-I antibody (MABF297, Merck) and anti-SNRP70 (SAB2102255, Merck) which served as a positive control.  Corresponding IgG control was also used as the negative control. RNA from immunoprecipitated fraction was extracted using TRI reagent. The library prep was done with the KAPA RNA HyperPrep kit, without ribosomal depletion. The input was 5-10 ng per sample for RIG-I RIP samples, and < 1ng for SNRP70 and IgG control samples. Nine PCR cycles were performed for library amplification. Samples were sequenced and the paired end reads were generated on NextSeq 2000.