GSM7233615: human milk; Homo sapiens; RNA-Seq
Human milk samples were collected at the University Hospital Zurich from healthy women, aged 20 to 40 years [mean (SD) 32.0 (5.0)], within 4 to 63 days after delivery. For isolation of milk cells, milk was diluted in a 1:1 ratio with PBS and centrifuged at 800 g for 20 minutes at 15°C. The lipid layer and skim milk were removed, and the cell pellet was washed twice in PBS by centrifugation at 400 g for 5 minutes and resuspension in PBS. 10X GEX 3`v3.1 Single index, Paired end 28_91 With Feature Barcoding