GSM7232984: MECP2.KO.rep2; Homo sapiens; RNA-Seq
Total RNA was collected using the RNeasy Mini Plus kit (QIAGEN). A fixed amount of ERCC RNA Spike-In (Invitrogen; 4456740) was added to each sample in proportion to the number of cells for RNA-seq normalization.  Samples were treated with NEBNext Poly(A) mRNA Magnetic Isolation Kit (NEB; E7490S) before library preparation using the YourSeq (FT & 3'DGE) Strand-Specific mRNA Library Prep (Active Motif; 23001) for full-transcript (FT) sequencing.