GSM8255816: Plate5_D05; Homo sapiens; RNA-Seq
Plasmocytoid dendritic cells were magnetically enriched from peripheral blood of a healthy donor, cultured with IL-3 and CD40L for two days and single-cell FACS-sorted on a FACS Aria Fusion (BD Biosciences) into Takara Smart-Seq Single Cell kit lysis buffer and frozen at -80C. The Illumina Nextera XT DNA Library Prep and Unique Dual Index Kits (Illumina) were used for library preparation according to manufacturer's instructions.