GSM7696574: airway basal stem cells, acetate treated Mpc2-deficient basal cells, H3 pan-ac, rep2; Mus musculus; OTHER
105 ALI-cultured basal cells were collected and bound to ConA beads for 10 minutes at room temperature. This was followed by an overnight incubation with the Histone H3ac (pan-acetyl) antibody at 4°C. After the secondary antibody incubation, the cells were mixed with 0.04 μM pA/G–Tnp for 1 hour at room temperature, and then subjected to tagmentation and DNA extraction. The libraries were constructed using the 'TD903 Hyperactive Universal CUT&Tag Assay Kit for Illumina' and the 'TD202 TruePrep Index Kit V2 for Illumina' (Vazyme).