GSM8366963: Mtz, 5dpf; Danio rerio; RNA-Seq
Larval intestines were manually dissected within an interval of 2 hours and collected in sterile HBSS-BSA 0.5%. Single-cell preparations were done by incubating dissected intestines in dissaggregation buffer (TrypLE supplemented with 0.5mg/mL of Dnase I) for 30min at 28°C, with extensive pipetting every 10 minutes. Single cell preparations were washed with HBSS-BSA 0.5%, filtered with a 70µm membrane, and stained with DAPI 0.2 mg/mL to exclude dead cells. Live intestinal cells were FACS-sorted using a Sony SH800S cell sorter. Library was prepared according to manufacturer's instructions (10x Genomics).