Illumina NovaSeq 6000 paired end sequencing; Characterization of enterotropic CD4 T cells reaching the tdLN after antibiotics
Characterization of enterotropic CD4 T cells reaching the tdLN after antibiotics
4 groups of mice : Control, antibiotics, antibiotics + 4days of recolonization, antibiotics + 4days of recolonization + Enterocloster clostridioformis. Tumor draining lymph node were harvested after CFSE injection were harvested and CFSE+ cells were sorted and proccessed in order to generate single cell RNA-sequencing using BD Rhapsody mouse immune response targeted panel. Groups were barcoded using BD Rhapsody Multiplexing Kit. BD Rhapsody pipeline and Seurat analysis BD Rhapsody pipeline and Seurat analysis BD Rhapsody pipeline and Seurat analysis BD Rhapsody pipeline and Seurat analysis BD Rhapsody Cartridge, cartridge reagent BD Rhapsody cDNA kit