GSM7697541: HFpEF-scr 1; Mus musculus; RNA-Seq
Left ventricular samples were harvested in ice-cold PBS, flash frozen on LN2, and RNA extracted using RNeasy kit (QIAzol, Qiagen). RNA-Seq library construction was performed using the Swift Biosciences RNA Library Kit (Swift Biosciences, Ann Arbor, MI), following mRNA purification using NEBNext® Poly(A) mRNA Magnetic Isolation Module. Final library concentration was measured via the Qubit 1X dsDNA HS Assay kit (ThermoFisher Scientific) and library size was evaluated on the 4200 TapeStation using the Agilent D1000 ScreenTape System (Agilent Technologies).