GSM8290599: E13.5 Motor neurons, ATAC, Control2; Mus musculus; ATAC-seq
Spinal cords were harvested from control and NFIAcKO E13.5 embryos carrying the Hb9::GFP transgene. Cells from each spinal cord were dissociated and sorted using a BD FACS Aria II Cell Sorter (UCSD Human Embryonic Stem Cell Core Facility), for GFP+ cells. Freshly-sorted cells were resuspended in cryopreservation solution and frozen at -80oC. Cells were incubated in permealization buffer (sigma) and centrifuge to obtain pemeabilized nuclei. 50,000 nuclei were tagmented and  purified using MinElute PCR purification kit (Qiagen). Libraries were amplified using NEB Next High-Fidelity 2X PCR Master Mix (NEB) and purified using MinElute PCR purification kit  (Qiagen). Two size selection steps were performed using SPRIselect bead (Beckman Coulter) at 0.55X and 1.5X bead-to-sample volume ratios, respectively. QC data processing was performed as described in Smith et al. 2021.