GSM7518385: Mouse_T1_dsRed; Homo sapiens; RNA-Seq
Spleen from infected mice were harvested and processed by homogenizing the spleen through 40um sterile filters. The cells were washed with 2mM EDTA containing PBS and treated with lysing buffer ACK (Life Technologies) to remove RBC. The remaining cells were stained with antibodies described in engraftment panel and flow sorted. Cells were sorted to enrich for GFP+ infected cells as described in results section. In 15 acutely infected cells, GFP+ HIV infected cells were pooled with dsRed+ and unmarked cells for 10x sample processing. 10d and 29d ART treated GFP+ latently infected cells were sorted into carrier wild type mouse B cells and processed for 10x sample processing. Unmarked and dsRed+ bystander cells were sorted separately and sequenced separately. FACS sorting followed by Genomics Chromium Next GEM Single Cell 3' Reagent Kits v.3.1 (CG000204 Rev D) protocol