GSM7108923: G47Δ responder 2 (R2); Mus musculus; RNA-Seq
The tumors were extracted day 5 and RNA was harvested using Rneasy mini plus kit (Qiagen). 1.3 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using NEBNext® Poly(A) mRNA Magnetic Isolation Module (Cat No. E7490) and NEBNext® Ultra II Directional RNA Library Prep Kit (Cat No. E7760) following manufacturer's protocols.