WGS of Pterocarpus santalinus
Genomic DNA was isolated from leaves (Genotype ID: BP; Table 1) using ArborEasy DNA isolation kit (Institute of Forest Genetics and Tree Breeding, Coimbatore, India). DNA concentration and purity was estimated using NanoDrop ND-1000 UV-Vis Spectrophotometer (Thermo Scientific, MA, USA) and DNA was quantified using Qubit Fluorometer (Thermo Scientific, MA, USA). DNA was purified using Agencourt AMPure XP reagent (Beckman Coulter, USA). Genomic DNA integrity was determined using Agilent Genomic DNA ScreenTape assay (Agilent Technologies, USA). The Illumina DNA library prep kit which uses bead based tagmentation was used to generate fragments of size of 300-350bp followed by post-tagmentation cleanup, indexing and PCR enrichment (5 cycles). The library was purified using sample purification beads following manufacturers protocol. The library was subsequently quantified using Qubit Fluorometer (Thermo Scientific, MA, USA) and size distribution was checked on Agilent Tapestation High Sensitivity D1000 ScreenTape system (Agilent Technologies, USA). The library was sequenced on Illumina NextSeq 2000 using 150 bp paired-end chemistry.