Acharia stimulea venom scoli
Total RNA was extracted using TRIzol, yielding 493 ng of RNA. Poly(A)+ RNA selection, TruSeq library preparation, and sequencing on a high-output run of an Illumina NextSeq 500 was performed by the Institute for Molecular Bioscience Sequencing Facility at The University of Queensland. This process yielded 26,482,164 150 bp paired-end reads, which were assembled de novo using an automated pipeline for trimming, correcting and assembling RNA-Seq reads using fqtrim, prinseq, and Trinity