Whole genome sequencing of Haloarcula hispanica AKS319 (pyrF trmB)
Culture was innoculated from a single colony into rich media (23% salt YPC with 50ug/mL uracil and 0.1% glucose (w/v)) and grown to mid-log phase at 37C. Genomic DNA was extracted using phenol-chloroform-isoamyl alcohol (25:24:1; Fisher Scientific) and ethanol precipitated. Purified DNA was quantified using a NanoDrop system (Thermo Scientific). Samples were submitted to Duke Center for Genomic and Computational Biology core sequencing facility for sonication and library preparation with TruSeq (Illumina) adapters.