GSM6532854: Kdm6b-cKO, H3K27me3, ChIP-seq, Rep 1; Mus musculus; ChIP-Seq
Cerebellum samples were dounced in a glass homogenizer containing 2 ml of 1% formaldehyde (w/v) PBS buffer per sample and kept at 25 °C for 15 min, washed twice with cold PBS, lysed in 600 μl lysis buffer (1% SDS (w/v), 10 mM EDTA, and 50 mM Tris, pH 8.1). The cross-linked material was sonicated with a Bioruptor (Diagenode), with output set to “high” with 30 s on/off cycles to an average size range of 150–350 bp which was then determined by agarose gel electrophoresis. ChIP-seq libraries were made using the MicroPlex Library Preparation kit V2 (C05010012, Diagenode); and 50 bp single-end sequencing was performed at the Duke Sequencing and Analysis Core Resource on a Hi-Seq 2000 machine.