GSM7696769: Baseline CD8+ T cells, cDNA; Homo sapiens; RNA-Seq
Live, CD3+CD8+ T cells were sorted of PBMC obtained pre-treatment and live, CD3+CD8+tetramer-APC+tetramer-PE+ T cells were sorted of PMBC obtained on-treatment and post-treatment with BD ARIA II cell sorter (BD Bioscience). Sorted cells were pooled and volume of cell suspension was diluted to 600 ml with BD Sample Buffer (BD Bioscience). Single cell capture and cDNA library preparations were performed using the BD Rhapsody system. First, BD Rhapsody cartridges were primed, and the pooled cells were loaded onto the cartridges and incubated at 4°C for 15 mins. The subsequent steps were performed according to the manufacturer's instructions (23-21336-01, BD Biosciences). Sample tag and cDNA libraries were generated for both the pre-treatment and on/post-treatment PBMCs (23-21712-00, BD Biosciences), while an additional TCR library was generated for on/post-treatment PBMCs (23-24018(01), BD Biosciences).