173 oyster digestive tissue RNA metatrascriptomic viromic sequencing data from 2016 to 2017
DTs of M. gigas were processed in FastPrep-24 (MP, USA) with two rounds of homogenizing (6 m/s, 30 secs). Total RNA was then extracted using TRIzol (Invitrogen, USA) and purified with total RNA rapid extraction kit (cat. GK3015, Generay, China). Extracted RNA from each batch were mixed for sequencing. NEBNext ultra II directional RNA library prep kit (cat. E7760S, Illumina, USA) was applied to library construction, during which, the prokaryotic rRNA was removed using the Ribo-zero rRNA removal kit (cat. MRZMB126, Illumina, USA). Established libraries were then sequenced through Illumina HiSeq (Illumina, USA) with 150 bp pair-ends reads generated.