GSM8118968: GSC 320-replicate2_CHIP; Homo sapiens; ChIP-Seq
5 million cells per replicate in each cell line were colelcted, tripzinyzed and crosslinked with 1% formaldehyde. Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared  using the KAPA Hyper prep kit (KAPA Biosystems) following the manufacturer's instructions. 10-30ng of DNA was used as starting material. Libraries were amplified using 8 cycles and then size-selected to isolate 250-450bp fragments with the Agencourt Ampure XP beads.