GSM7031549: 2_AK481_sema3clow, biol rep 1; Homo sapiens; ATAC-seq
Nuclei from early-, late-, and non-invaders were extracted from 50,000 lysed cells and collected in lo-bind tubes. DNA tagmentation was performed using the Illumina Tagment DNA Enzyme TDE1 (20034197). Libraries were prepared using custom primer indices follwed by double-sided bead purification using Agencourt AM Pure XP magnetic beads (A63880). Libraries were tested for average fragment length using Agilent High Sensitvity DNA Kit (5067-4626) and the concentration was tested using Qubit™ dsDNA HS kit (#Q32851, Invitrogen). ATAC-seq; Samples were run as paired-end using 150-cycle NextSeq 500/550 High Output Kit v2.5.