RNA-Seq of metagenome
After extracting genomic DNA from the samples, the conserved regions of rDNA were amplified using specific primers with barcode. The PCR amplification products were then cut and recovered, and quantified by QuantiFluorTM fluorometer. The purified amplification products were mixed in equal amounts, ligated to sequencing junctions, sequencing libraries were constructed, and Illumina PE250 was up-sequenced.