GSM7885407: Donor 2 - 2D; Homo sapiens; RNA-Seq
RNA was extracted from the fibroblasts using 2-Mercaptoethanol and RA1 lysis buffer and purified using the NucleoSpin RNA Kit according to the manufacturer's protocol. RNA quantity and quality were checked using the TapeStation system and RNA integrity number and RNA concentration were calculated using TapeStation Analysis Software 3.2. The NEBNext Ultra II Directional RNA Library Prep Kit for Illumina was used to process the samples.