GSM6482263: HK2, HIF1, Normoxia; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using an ultrasonicator. ChIP-seq libraries were prepared using the Truseq v5 Library Preparation Kit. 10 ng of DNA was used as starting material for input and ip samples. Libraries were amplified on the thermocycler. Post amplification libraries were size selected at 250-450bp in length. Libraries were validated using the Agilent High Sensitivity DNA Kit.