GSM8031364: T-5498, ST; Homo sapiens; OTHER
Following 10x Visium Spatial Protocols – Tissue Preparation Guide (CG000240), OCT embedded tissue was scored to the size of the capture area targeting the SN. One 10 µm section was mounted on each capture area of the Visium slide. Tissues on the slides were fixed using a methanol-containing buffer as per the 10X Visium manual, stained with H&E or antibodies NeuN, GFAP, and DAPI as per the 10X protocol for Immunofluorescence Staining & Imaging for Visium Spatial Protocols (CG000312), and then imaged. Imaging of whole slides was done at 20X magnification on a Leica DMI8 Thunder microscope. After imaging, the slides were de-cover-slipped and the tissue was permeabilized for 11 minutes (which was empirically determined to yield best results based on the Visium Spatial Tissue Optimization Slide & Reagent Kit PN-1000193, as detailed in the protocol provided in document CG000238_RevD available in 10X demonstrated protocols). libraries were prepared using Visium Spatial Gene Expression Slide & Reagent Kit, 16 reactions (PN-1000184). Visium Spatial Gene Expression Reagent Kits user guide (CG000239 Rev G) was followed.