GSM6754837: Bill skin Replicate 6; Anas platyrhynchos; RNA-Seq
Total RNA was extracted using Quick-RNA Microprep Kit (Zymo) according to manufacturer's instructions. RNA concentration and integrity number (RIN) were assessed by Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA). RNA concentrations for corpuscles were in the range of 63–323 pg/µl and RIN values were in the range of 6.1–8.0. Sequencing libraries were prepared using the Kapa mRNA Hyper Prep kit (KAPA Biosystems, Wilmington, MA) (skin samples) or the NEBNext Single Cell/Low Input RNA Library Prep Kit (New England Biolabs, Ipswich, MA) (Meissner corpuscles samples).