WGS of Zygosaccharomyces bailli isolate from Kombucha Tea
Mannitol growth media supplemented with 1% D-glucose and 1% glycerol was inoculated with the KT associated microbes and the cultures were grown for 48 hrs at 25C with shaking. The gDNA was isolated from cell pellets using the Wizard Genomic DNA Purification kit (Promega, A1120). Preparation and Illumina short read sequencing (PE 150) of DNA-Seq libraries was performed by Novogene (Sacramento, CA).