GSM7109792: PIM6 1; Homo sapiens; RNA-Seq
Cells were harvested in RLT buffer (Qiagen) and extracted using an Rneasy kit (Qiagen). mRNA was then isolated with an NEB Next Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Libraries were constructed using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina, Index Primers Sets 3 and 4 (New England Biolabs)