GSM8366251: scRNA-seq of pooled anti-TGF-beta antibody-treated cells derived from flow cell 1; Mus musculus; RNA-Seq
Cells were concentrated to 1000 cells/ml and loaded onto the Chromium Controller (10X Genomics, Pleasanton, CA) with a target of 10,000 cells per sample. Two captures per treatment condition were performed. Cells were mixed with barcoded gel beads and 5' GEM Kit v3 reagents and single cell capture was performed (10X Genomics). Following reverse transcription, cDNA and murine TCRs were amplified, and gene expression and TCR sequencing libraries were constructed according to the manufacturer's recommendations. Each DNA library was loaded into a sequencing lane on a NovaSeq system (Illumina, USA) and was sequenced with pair-end reads of 75 bp.