GSM7996229: EC_TCD,  mRNA-derived cDNA; Mus musculus; RNA-Seq
Cells were isolated from liver following enzymatic digestion, pooled per group (n = 3 mice), stained with CD3-PECy7, CD45-BUV395, TER119-APC and 7-AAD and sort-puried to CD31+ endothelial cells. Samples were resuspended at 1000 cells/uL and libraries  were  generated  using  the Chromium Single Cell 5' Reagent Kits User Guide (v2 Chemistry Dual Index) following the manufacturer's protocol (CG000331, Pleasanton, CA). Final library quality was assessed using the Tapestation 4200 (Agilent, Santa Clara, CA) and libraries were quantified by Qubit dsDNA kit on Denovix( spectrometer/flurometer) .Pooled libraries were then subjected to paired-end Illumina sequencing according  to the  manufacturer's  protocol