GSM6857622: RNA_Smarcc1 #1; Mus musculus; RNA-Seq
RNA was isolated from organoids using RNeasy Micro Kit (Qiagen). RNA-seq libraries were generated with the TruSeq RNA Library Prep Kit (Illumina). The concentration and quality of the library was measured using Qubit and Bioanalyzer. The library was diluted to 2 nM and a final concentration of 1.7 pM was sequenced with Illumina High Output 75 cycles kit on an Illumina NextSeq 500 sequencer