metatranscriptome of soil co-contaminated with hydrocarbons and heavy metals subjected to enhanced phytoremediation
Ribosomal RNA depletion was performed using NEBNext rRNA Depletion Kit (Bacteria) (New England BioLabs, Germany), the library was constructed using TruSeq Stranded Total RNA (Illumina, San Diego, CA, USA). The libraries were sequenced using Illumina NovaSeq 6000 (2x150 bp, paired-end, Illumina, San Diego, CA, USA).