1TS-DNA-Seq of soil:SW_WW_GS
Undried bulk soil samples were collected from experimental plot and genomic DNA extracted in triplets using PowerLyser PowerSoil DNA Isolation Kit (MO BIO Laboratories, Carlsbad, CA, USA) following the manufacturers protocol, modified by starting with initial soil mass approximately 0.5g and by use of a bead beater for cell lysing. The ITS2 marker region was PCR amplified using Illumina adaptor ligated universal primers  ITS7(5-GTGAATCATCGAATCTTTG-3) and ITS4(5-TCCTCCGCTTATTGATATGC-3) from each triplet. The thermocycler condition was maintained at 94oC for 3 min, followed by 25 cycles of 94oC for 30 s, 55oC for 30 s, 72oC for 30 s and a final extension of 72oC for 3 min.