GSM7916579: GS.0906 (3DD-GSC) [ERA-MC-74]; Homo sapiens; RNA-Seq
Cell cultures were washed with PBS and incubated with Accutase (Gibco) for 10-15 minutes, and collected in HBSS. Cells were centrifuged and resuspended in HBSS (Gibco), and subsequently sorted with FACSAria II (BD Biosciences) into 384-well plates based on single cells selection gating. Cells were then subjected to single cell transcriptomic sequencing (SORT-seq) The libraries were constructed according to the SORTseq protocol. Briefly, single cells sorted into 384-well plates were submitted for scRNAsequencing at Single Cell Discoveries, Utrecht. Libraries were constructed based on a semi-robotized implementation of the CELseq2 library protocol, and submitted for paired-end sequencing