GSM7775334: Vehicle_treated 3; Mus musculus; RNA-Seq
Mice were deeply anesthetized, and the entire spinal cord were dissected and snap-frozen in powdered CO2 in acetone. We dissected the mice at the age of 16 weeks.Total RNA was extracted from the thoracic spinal cord using the InvitrogenTM TRIzolTM Plus RNA Purification Kit. A library was independently prepared with 1ug of total RNA for each sample by Illumina TruSeq Stranded mRNA Sample Prep Kit (Illumina, Inc.)