DNA-Seq of soil metagenome: PBS_15_biofilm
The MPs was handpicked from the sediments using sterile tweezer and rinsed three times to remove the dirt attached. Microbial genetic DNA was extracted from the sediments and MPs. The quality of the DNA extract was examined via 1% agarose gel electrophoresis. Then V4 region of 16S rRNA gene was amplified using primer set of 515F and 806R. The PCR products was recovered using AxyPrepDNA gel extraction kit (AXYGEN, USA), and then quantified using QuantiFluor dsDNA system (Promega, USA).