M14 3D CD20+ replicate 3
M14 cells were grown under non-adherent culture conditions to generate melanospheres. Melanospheres were mechanically dissociated, antibody labelled for CD20 and flow sorted to isolate CD20+ and CD20- populations. RNA was extracted from samples using an RNeasy mini kit as per manufacturers instructions. RNA was then quantified and quality assessed before being sent to Novogene (Cambridge, UK).