GSM6754880: DKO, ZFXWT, FLAG, rep1; Homo sapiens; ChIP-Seq
Chromatin was prepared using the protocol previously published (PMID: 29429977). Briefly, cells were crosslinked by 1%formedelhyde for 10 mins and lysised into nuclei. Nuclei were sonicated using BioRuptor to break into chromatin fragments. Libraries were prepared using KAPA Hyper Prep Kit (Kapa #KK8505) according to provided protocol