GSM6533105: SupT1, Active, ATAC, rep2; Homo sapiens; ATAC-seq
100,000 cells were used to for transposition reaction (Illumina Tagment DNA TDE1 Enzyme and Buffer Kit). Tagmented DNA was purified with MinElute purification kit (Qiagen) Tagmented DNA used for subsequent library preparation by PCR amplification using unique dual-indexing (Illumina Nextera i5 common adapter and i7 index adapter) primers. ATAC-seq libraries were purified using AMPure SPRI beads (Beckman Coulter)