GSM7232941: Forebrain, control, replicate 1; Mus musculus; RNA-Seq
At week 5, animals were perfused with PBS containing 0.005% heparin, hemi-forebrains were collected and tissue was homogenized according to the Adult Brain Dissociation Kit (Miltenyi Biotec). Cells were further processed using 10x Genomics reagents and loaded into the 10x Genomics Chromium Controller. Libraries were prepared for NGS Illumina Next Seq (NovaSeq S2 PE100) using the Chromium Next GEM Single Cell 3' Kit v3.1 and following the manufacturer instructions.