GSM8330438: Microglia_US_Sympt_biol rep 5; Mus musculus; RNA-Seq
RNA was extracted using the RNeasy plus micro kit (Qiagen). RNA quality was determined with TapeStation 2200, Agilent Whole total eluted RNA of microglia (between 400-700 pg)   was used to perform cDNA synthesis using the Smart-Seq v4 Ultra Low Input RNA kit for sequencing (TaKaRa) with linear pre-amplification. Library preparation was made with 150 pg of amplified cDNA for every sample using the Nextera XT DNA Library Preparation Kit (Illumina). Samples were sequenced on a NovaSeqXplus sequencing system (Illumina) with an average coverage of 2X50 millions of reads per sample (150bp, paired-end) after demultiplexing.