GSM7995076: 786-O,p65_Veh,ChIP; Homo sapiens; ChIP-Seq
Cells were cross-linked by 1% warm formaldehyde for 10 min. After quenching and cell lysis, chromatin was sonicated using a water bath sonicator (Diagenode bioruptor). We use 1x107 cells for one ChIP reaction. DNA was purified using the ChIP DNA Clean & Concentrator kit (D5205, Zymo Reasearch), and then subjected to Illumina ChIP-Seq library construction using VAHTS Universal Pro DNA Library Prep Kit (ND608,Vazyme).Post amplification libraries we're size selected at 300-700 bp in length using AMPure XP breads from Beckman Coulter. Libraries were validated using the Aglient 2100 Bioanalyzer.