GSM7696655: PDXC-OS052 DMSO-2 [ATAC-seq]; Homo sapiens; ATAC-seq
cells were first lysed in buffer (10mM Tris-HCl pH7.4, 10mM NaCl, 3mM MgCl2, and 0.1% Igepal CA-630). Then, cell pellets were incubated with transposition reaction mix (25µl Tagment DNA Buffer, 2.5µl TDE1 Tagment DNA Enzyme, and 22.5µl nuclease-free H2O) (Illumina, 20034197) for 30 min at 37°C in a thermomixer at 300rpm, followed by PCR reaction for 18 cycles.