GSM7863621: High dose, Day 1, NHP 5; Macaca fascicularis; RNA-Seq
Whole-blood (250 µL) collected 18 days prior to vaccination and at 1- and 3-days post vaccination were stored at -80°C in DNA/RNA Shield (Zymo Research). Total RNA was isolated from whole blood samples using the Zymo Research Quick RNA Whole Blood Kit according to the manufacturer's protocol. Sample libraries were globin-depleted and prepared using the SMART-Seq® stranded kit (Takara Bio) which includes poly(A) selection.