GSM7697325: PVT-First; Mus musculus; RNA-Seq
Mice were anaesthetized with 2% isoflurane, and then the brains were rapidly removed. The brain was frozen using a freezing microtome (CM1850; Leica, Buffalo Grove, IL, USA) and then cut into slices (~300 μm). The PVT tissues were taken quickly and stored in liquid nitrogen. Library was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics).