GSM8254522: Fetal lung organoids derived from BU3 PSC; Homo sapiens; RNA-Seq
hPSC-derived cultures were dissociated into single cells using 0.25% trypsin at 37°C for 10 minutes and deactivated using 1% BSA in PBS. Cells were multiplexed using MULTI-seq and barcode library preparation as previously published (McGinnis et al., 2019) . In brief, pre-designed DNA barcodes were hybridized to the plasma membranes through an anchor lipid-modified oligonucleotide (LMO). Subsequently, library construction was done using the 10X Chromium Next GEM single-cell 3' Reagent Kits v3.1 (10x Genomics). Library prep was performed as per manufacturer's protocol. During cDNA amplification, barcode fraction was also collected and a separete library preparation was done for the barcodes.