GSM8068852: DMSO, CDKI-73, 48h, rep1; Homo sapiens; ChIP-Seq
After 48 hours, cells were fixed with formaldehyde. Nuclear pellets were sonicated on ice for 10 cycles (30 sec on, 30 sec off) using a Diagenode Bioruptor. Lysates were immunoprecipitated with 2 ug H3K27ac antibody (Abcam ab4729). DNA was purified using phenol:chloroform extraction and isopropanol precipitation. Libraries were prepared from 5 ng of ChIP DNA using a Qiaseq UltraLow Input Library Kit (Qiagen)according to the manufacturer's instructions.