GSM6481396: tIMEC_RNA_3; Homo sapiens; RNA-Seq
Cells were directly lysed on plates, and tissue collected from primary lung tumours (PT) was lysed with TRIzol (Thermo Fisher cat. #15596026), and total RNAs were extracted according to the manufacturer's instructions. ontaminating genomic DNA was removed by DNase (Qiagen cat. #79254) digestion. RNA quality and concentration were assessed using the 2100 Bioanalyzer (Agilent cat. #G2939BA) and the Qubit fluorometer (Thermo Fisher cat. #Q33226), respectively. RNA-seq libraries were prepared by using the TruSeq® Stranded Total RNA (Illumina #20020596) supplemented with Illumina Ribo-Zero plus rRNA Depletion Kit (Illumina #20037135) starting from 500 ng of total RNA.Libraries were sequenced on an Illumina HiSeq 2500 with SE 50 bp. 