GSM6482094: NB41; Homo sapiens; RNA-Seq
Prior to RNA extraction tumor cell content was checked by a pathologist and only samples with >60% tumor content were processed further. 30-60mg of snap-frozen neuroblastoma specimen was cryo-sectioned and were homogenized in TRIzol reagent (Invitrogen, Karlsruhe, Germany) using the FastPrep FP120 cell disruptor (Qbiogene, Inc, Carlsbad, CA, USA).Total RNA was isolated following the TRIzol protocol (Invitrogen) and RNA integrity was assessed using the 2100 Bioanalyzer (Agilent, Waldbronn, Germany). Only samples with an RNA Integrity Number >7.5 were taken for further analysis. Library construction was performed according to the standard TruSeqTM protocol.