GSM8255199: AM, LPS+IFN-γ+ONX0914, ATAC; Mus musculus; ATAC-seq
Fragmented DNA products were extracted using ATAC DNA Extract Beads. Amplified products were purified using ATAC DNA Clean Beads through a two-step sorting protocol (0.55×/1.2×). For ATAC-seq, 100,000 cells were lysed in chilled Lysis Buffer, and sequencing libraries were prepared using the Hyperactive ATAC-Seq Library Prep Kit for Illumina (Vazyme, TD711, Nanjing, China) following the manual protocol.