RNA-Seq of wetland metagenome
RNA was extracted with RNeasy PowerSoil Total RNA Kit. Total RNA samples were polyadenylated using Escherichia coli poly(A) polymerase, purified by GeneJET RNA Cleanup and Concentration Micro Kit, reverse RNA was extracted with RNeasy PowerSoil Total RNA Kit. Total RNA samples were polyadenylated using Escherichia coli poly(A) polymerase, purified by GeneJET RNA Cleanup and Concentration Micro Kit,. Kapa Hyper prep with PCR