GSM8031831: PW_006_IC_rep2; Homo sapiens; RNA-Seq
RNA was isolated from blood neutrophils after overnight culture with poly(I:C) using the Nucleospin RNA II kit with on-column genomic DNA digestion according to the manufacturer's protocol (Takara Bio, San Jose, CA). RNA was quantified using a NanoDrop Fluorospectrometer (Thermo Fisher). RNA was quality assessed using the 2100 Bioanalyzer (Agilent, Santa Clara, CA) in the Emory Integrated Genomics Core. cDNA was generated using the SMART-Seq® v4 Ultra® low Input RNA Kit (Takara Bio). The final RNA-seq library was generated using the Nextera XT kit (Illumina, San Diego, CA). RNA-seq libraries were sequenced on the Novaseq 6000 with a sequencing depth of 30M paired-end 100 base pair reads per sample.