GSM7886246: Ascending aorta, AD1; Mus musculus; RNA-Seq
The Single Cell 3' Protocol upgrades short read sequencers to deliver a scalable microfluidic platform for 3' digital gene expression profiling of 500–10000 individual cells per sample. The 10x™ GemCode™ Technology samples a pool of ~750000 barcodes to separately index each cell's transcriptome. It does so by partitioning thousands of cells into nanoliter-scale Gel Bead-In-EMulsions (GEMs), where all generated cDNA share a common 10x Barcode. Libraries are generated and sequenced from the cDNA and the 10x Barcodes are used to associate individual reads back to the individual partitions. R1 (read 1 primer sequence) are added to the molecules during GEM incubation. P5, P7, a sample index, and R2 (read 2 primer sequence) are added during library construction via End Repair, A-tailing, Adaptor Ligation, and PCR. The final libraries contain the P5 and P7 primers used in Illumina bridge amplification.