Targeted capture EM-seq of Homo sapiens : whole blood
Targeted capture panel was designed to tile the selected bivalent domains (10bp flanking). 4,200 hybrid capture probes using the Twist target enrichment (Twist Bioscience, San Francisco, CA, USA) were synthesized to capture ~58,000 CpGs within the selected bivalent domain regions. Genomic DNA samples were fragmented physically by Covaris (200 to 300bp). Methylated cytosine residues of initial 200ng input gDNA were converted enzymatically by Twist Biosciences NEBNext Enzymatic Methyl-Seq (EM-Seq).  Then pre-PCR amplification and sample library preparation were processed. Twist fast hybridization target enrichment with 8-plexing, post PCR amplification, and libraries were sequenced on DNBSEQ-G400 Dx (MGI Tech, Shenzhen, CHINA) with 100bp paired-end reads with a minimum coverage of 280x (average coverage 380x; 234x~579x).