Metagenomics of uricase-deficient mus musculus: cecal contents
Following extraction, purified genomic DNA was quantified using a Qubit fluorometer (ThermoFisher). Between 100-500ng of each sample were taken forward to construct metagenomics sequencing libraries using the Illumina DNA Prep kit, with half-volumes being utilized at each step to minimize cost. Post PCR, libraries were purified using a 0.8x bead clean, and were quantified again using the Qubit. Equal masses of each metagenomics library were pooled, and a dual-sided AMPure XP (Beckman Coulter) bead clean was performed on the pooled material to ensure proper size-selection for sequencer loading. Sequencing was performed on the NovaSeq6000 (Illumina) using a PE155 read configuration.