RNAseq of homo sapiens: single cultured cells
Bioprtined samples were first digested to collect the cells. Trizol was immediately added to cell pellets to preserve RNA integrity. Total RNA was extracted using Zymo Microprep kit. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.