GSM7518891: purifed 2W1S specific CD4 cell from Ca-2W1S colonized mice (April 2016); Mus musculus; RNA-Seq
For the initial cell capture, cDNA synthesis and amplification, and harvest step, the Core Staff uses the SMART-Seq® v4 Ultra Low Input RNA Kit for the Fluidigm® C1™ System, IFCs User Manual and follows all manufacturer's instructions. For the Library Preparation, Pooling and Cleanup, again the Core Staff follows the manufacturer's instructions from the SMART-Seq® v4 Ultra Low Input RNA Kit for the Fluidigm® C1™ System, IFCs User Manual. The only change is the time of the tagmentation reaction. The Core staff using the following Tagmentation Step: 55 C – for 20 minutes.