GSM7863805: bacteria in mineral water, day 0, replicate 3; Listeria monocytogenes EGD-e; RNA-Seq
Total RNAs were recovered from bead-beaten bacterial cells using a phenol/chloroform extraction method. The quality of RNA extracts was assessed in an Agilent Bioanalyzer 2100, using the RNA 6000 pico kit (Agilent Technologies). Total RNAs (450ng) were treated with DNase (Baseline-ZERO DNase, Epicentre). Library construction -including ribosomal RNA depletion- and sequencing was performed by I2BC sequencing facility (Gif-sur-Yvette). Ribosomal RNA was removed using the Illumina Ribo-Zero Magnetic Kit (Bacteria), according to the manufacturer recommendations. Directional RNA sequencing libraries were constructed using the TruSeq Stranded Total RNA Library Prep kit (Illumina), and library quality was assessed on an Agilent Bioanalyzer 2100, using the Agilent High Sensitivity DNA Kit (Agilent Technologies). Libraries were pooled and sequenced in 2×75-bp paired-end runs on a NextSeq500 instrument, using a NextSeq500 High Output 150 cycles kit (Illumina).