GSM7776181: 1BR3_03; Homo sapiens; RNA-Seq
Total RNA from was isolated with the Qick-RNA MicroPrep Kit (Zymo Research), which includes a DNAse-Digestion step. Libraries were prepared using NEBNext Ultra II Directional RNA Library Preparation Kit in combination with NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors RNA) following the manufacturer's instructions (NewEngland Biolabs).