GSM8207858: Cardiomyocytes, DMSO1h, C1; Cavia porcellus; RNA-Seq
Total R.N.A. extraction was conducted on isolated guinea pig ventricular cardiomy-ocytes exposed independently to 10 μM of DOX and to the vehicle control (DMSO) for 22 hours at room temperature. We used Trizol reagent to extract following the manufacturer's guidelines (Invitrogen). The extracted R.N.A. was resuspended in H2O DEPC (diethyl py-rocarbonate, Sigma–Aldrich) and quantified via spectrophotometry using the Nanodrop 2000 (Thermo Fisher Scientific). A small R.N.A. library was constructed using the TruSeq Small R.N.A. Library Prep Kit (Illumina Inc., U.S.A.). . The quality control and the normalization of the readings obtained using the BaseSpace® App, Small RNA v1.0 (Illumina), the sequences converted into FastQ files, and the quality of the readings was analyzed multiQC and confirmed in Galaxy by FastQC (https://usegalaxy.org/). TruSeq Small R.N.A. Library to miRNAs was sequenced on an Illumina HiSeq2500 platform. Quality control and normalization of the resulting reads was performed using the BaseSpace® Small RNA v1.0 app (Illumina), with subsequent conversion of the sequences to FastQ files.