RNA-Seq of pearl millet: leaves of plants exposed to a 12-h cold treatment
mRNA was quality-checked, enriched by oligo(dT) beads, and fragmented randomly by a fragmentation buffer. The cDNA was synthesized by the mRNA template, random hexamers, a custom second-strand synthesis buffer (Illumina), dNTPs, RNase H and DNA polymerases. I are added to initiate the second-strand synthesis. A series of terminal repair and sequencing adaptor ligation was applied to complete the cDNA library preparation.