GSM7864357: HEK-293T cells, mock transfected,  26h, replicate 1; Homo sapiens; RNA-Seq
Cells were collected at eight, 12 and 26 hours after transduction, media was discarded and cells were washed with 500 µl PBS, and then suspended into 500 µl TRIzol Reagent (Thermo Fisher Scientific, MA, USA). RNA isolation was carried out according to the manufacturer's instructions. RNA quality was determined by Agilent RNA 6000 Nano kit on an Agilent 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany). Thereafter,  sequencing was performed on the DNBSEQ G400 sequencer using MGIEasy RNA Library Prep Set.