GSM6857342: Peds NIBD Colon 46; Homo sapiens; miRNA-Seq
Small RNA libraries were generated using Illumina TruSeq Small RNA Sample Preparation Kit (San Diego, CA). miRNA was enriched from FFPE tissue samples using the Roche High Pure miRNA Isolation Kit (Penzberg, Germany). RNA purity and integrity were assessed with Thermo Scientific NanoDrop 2000 (Waltham, MA) and Agilent 2100 Bio- analyzer (Santa Clara, CA), respectively. Single-end (50 bp) sequencing was performed on the Illumina HiSeq 2500 platform.