GSM8366964: Pleuroperitoneal folds, GD 13.5, Rep 1; Mus musculus; RNA-Seq
Pleuroperitoneal folds from a mouse embryo were carefully dissected at GD 13.5. Following this, the tissue was incubated in 0.25% trypsin-EDTA for approximately 10 minutes at 37 degrees celsius. Trypsin was inactivated by adding 1 mL of 10% Fetal bovine Serum, and the solution was centrifuged for 5 minutes at 340xg. The supernatant was discarded and the pellet was resuspended in 1 mL of 10% fetal bovine serum. The cells were sent to the high content analysis core at the University of Alberta and the libraries were generated using the Chromium Next GEM single cell 3' Gene expression v3.1