GSM8328968: h- 972 cells, wild type, minimal medium w/o nitrogen 4 hrs, rep 2; Schizosaccharomyces pombe; RNA-Seq
Cells were washed with cold DEPC-H2O and snap freeze. RNA extraction was carried out by disrupting the cells with cells with glass beads in TRIzol® Reagent (Invitrogen) and following the manufacturer's instructions. Library preparation using the Illumina TruSeq Stranded Total RNA and subsequent NGS sequencing was performed by Macrogen.