GSM7697287: BM,replicate 2,  scRNA-seq; Mus musculus; RNA-Seq
Spleen and bone marrow were isolated from the mice. Single cell suspensions were generated by mecanical dissagregation of the spleen agaisnt a 70 micrometers strainer and by flushing the bone marrow with 3 ml of complete RPMI medium. Cells were passed through a MACS-LS column to remove the non-targeted cells by using monocyte isolation kit. Library was performed according to manufacturer's instructions (10x Genomics Chromium Controller and the Chromium Next GEM Single cells 3' Library Kit v3.1 )