GSM8118807: ChIP input, 24h starvation, HEK293T, Rep2; Homo sapiens; ChIP-Seq
Cells were fixed using 1% formaldehyde for 5 min. The crosslinking reaction was quenched with 125mM glycine. Cells were lysed in swelling buffer 5mM PIPES pH8; 85mM KCL; 0,5% IGEPAL + protease inhibitors). Chromatin was sonicated on a Diagenode Bioruptor300 for 30 min (30s ON/ 30s OFF) on the high setting. At least 20 mg of DNA were used for library preparation using the NEBNext Ultra DNA library prep kit. ChIPseq, 150 bp paired-end reads