GSM7109665: Treg KO2; Mus musculus; RNA-Seq
RNA was prepared using miRNeasy Mini kit (Qiagen) according to the manufacturer instructions. RNA from the in vitro differentiated CD4 T cells was assessed by Bioanalyzer 2100 with the Agilent RNA 6000 Pico Kit (Agilent). cDNA libraries were made using the TruSeq Stranded mRNA kit (Agilent) and sequenced using Illumina NovaSeq S4-300 flowcell, 2x150 bp paired end, in NGI Stockholm.