GSM6529452: ChIP-SCC25-input-5.25; Homo sapiens; ChIP-Seq
Cells for ChIP were cultured in 15 cm plates. Cells were first washed with cold PBS, crosslinked at room temperature with 1% formaldehyde (ThermoFisher Scientific) for 10 min.  Genomic DNA was purified with DNeasy kit (QIAGEN). For ChIP–seq, NEBNext Ultra Directional RNA Library Prep Kit (NEB, #E7760) was used for library preparation. For MeDIP–seq, IP DNA and input DNA were PCR amplified with Illumina indexing primers.