GSM8366833: R. tolerans EL-164, prior to AHL spike-in, NAME- and AHL-spiked cultures, 49 h incubation time, replicate B; Roseovarius tolerans; RNA-Seq
For RNA sampling, 2 ml EL-164 culture were removed aseptically, snap frozen in liquid nitrogen and subsequently stored at -80 °C until RNA extraction. The latter was performed as described earlier (Leinberger et al. (2022)), omitting the DNA extraction steps. Libraries for transcriptomics were generated according to Shishkin et al., 2015 including rRNA removal with the RiboZero Kit (Illumina Inc., San Diego, CA, US).