ChIP sequencing experiment of HA-tagged WT CDK6 or of a C-terminally truncated variant of CDK6 (CDK6 delta-C) in BCR-ABL1 expressing cell lines
ChIP sequencing experiment of HA-tagged WT CDK6 or of a C-terminally truncated variant of CDK6 (CDK6 delta-C) in BCR-ABL1 expressing cell lines
Cells expressing CDK6_WT, CDK6_deltaC, as well as CDK6_KO cells were harvested from cell line culture. Cells were crosslinked with DSG (20 minutes, RT) and 1% formaldehyde (10 minutes, RT) and termination of the fixation procedure was performed using glycine. For immunoprecipitation (IP), 70 Âµl Dynabeads Protein G magnetic beads (Invitrogen) were used. The antibody against the HA tag was ab9110 (Abcam).  Input DNA or DNA enriched through IP was subjected to sequencing library preparation using the NEBNext Ultra II chemistry (New England Biolabs)