GSM7036286: H1-CAR-D0 biol rep 1; Homo sapiens; RNA-Seq
Day 0, 4, and 7 ATOs were harvested into single cell suspensions in MACS buffer (PBS/0.5% bovine serum album/2mM EDTA) by mechanical dissociation. Live cells were first enriched with the Dead Cell Removal Kit (Miltenyi Biotec, Cat 130–019-101). Cells were then stained and FACS-sorted as DAPI- mouse CD29- (Biolegend, San Diego, CA) to deplete residual dead cells and MS5 stromal cells, respectively. 10X 3' RNAseq library generation from sorted cells was performed by the UCLA Technology Center for Genomics and Bioinformatics (TCGB) Core using the 10X Genomics Chromium™ Controller Single Cell Sequencing System (10X Genomics, Pleasanton, CA), per the manufacturer's instructions and TCGB Core standard protocol