GSM7885932: WTnoIR, H3K27me3, regen, rep1; Mus musculus; OTHER
Mouse intestine was harvested and epithelial cells were eluted with 20 mM EDTA + 1 mM DTT and filtered through 100 and 40 um cell strainers. Nuclei were isolated from cells following Heinekoff et al. "Benchtop CUT&Tag". Organoids were harvested using EDTA to remove matrigel and TrypLE to digest to single cell, at which point nuclei were isolated following H3inekoff et al. "Benchtop CUT&Tag". CUT&Tag was performed as in Heinekoff et al. "Benchtop CUT&Tag" for each antibody. Libraries were amplified using 13 cycles on the thermocycler. Post amplification libraries were purified using Agencourt AMPure XP beads from Beckman Coulter. Libraries were validated using Agilent High Sensitivity DNA kit.