GSM6857915: ATAC_transferred_Th2_48h_TxnipKO_rep2; Mus musculus; ATAC-seq
The scRNA-seq samples of Th2 at day7 and day11 in vivo that were freshly prepared from KJ1+CD4+ T cells sorted using a BD FACS Aria III (10,000 cells each, cell viability >98%). The Effector Th2 and transferred Th2 that were also freshly prepared from KJ1+CD4+ T cells sorted using a BD FACS Aria III (10,000 cells each, cell viability >98%). The scRNA-seq , RNA-seq and ATAC-seq samples of Wild-type or Txnip KO mice that were also freshly prepared from CD4+CD44high T cells sorted using a BD FACS Aria III (10,000 cells each, cell viability >98%). Libraries were prepared using Chromium Single Cell 3' Reagent Kits v3.1 according to manufacturer's protocol (10X Genomics). The generated scRNA-seq libraries were sequenced using a 128 cycle (paired-end reads) with a NovaSeq 6000 (Illumina).