GSM6755082: 120820_PY01_liver; Trichosurus vulpecula; ssRNA-seq
250 ng of DNase-treated RNA was used to construct poly(A) enriched, strand-specific RNA sequencing libraries using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina and NEBNext Poly(A) mRNA Magnetic Isolation Module (¼ of the standard reaction volume) (NEB) Total RNA was extracted from tissue samples (~50 mg) stored in RNAlater using TRIzol Reagent (Invitrogen) following the manufacturer's guidelines. RNA samples were treated to remove any contaminating DNA using the Turbo DNA-free kit (Invitrogen) according to the protocol.