RNA-sequencing of aerial parts of flax (Linum usitatissimum) after treatments with COS-OGA elicitors and infection with Verticillium dahliae
RNA were extracted from 50 mg with the kit NucleoSpin RNA Plant (Macherey-Nagel, Germany). RNA concentration, quality, quantity, integrity, and purity were checked using Nanodrop (Isogen, ND-1000, Netherlands) and Bioanalyzer 2100 (Agilent, United States). TruSeq Stranded mRNA kit (Illumina, United States) was used on 1 g total RNA to prepare the library. Libraries were sequenced on NovaSeq6000 platform (Illumina, United States) following the 100 bp single-end reads protocol (NovaSeq6000 S1 Flowcell 100 cycles kit) at the VIB Nucleomics core.