MSD02-MS-E04
Dual-indexed Illumina sequencing libraries were constructed using 100 ng of genomic DNA as input into the 2S Turbo kit (Swift Biosciences) as per the manufacturers instructions except that reaction volumes were conducted  in 1/5 of the standard volume, the post-ligation bead clean up step was eluted directly into the PCR master mix, and the beads were left in the reaction during the 18 cycle PCR reaction.