GSM7994744: H3K4me3 cKit CKO ChIP rep 1; Mus musculus; ChIP-Seq
RNA was extracted from the enriched spermatogonia with the use of a PureLink RNA Mini Kit and the preparation of a cDNA library was conducted by Rhelixa Co., Ltd using NEBNext® Poly(A) mRNA Magnetic Isolation Module (Cat No. E7490) and NEBNext® Ultra™II Directional RNA Library Prep Kit (Cat No. E7760). ChIP was subjected with antibodies to CHD8 or H3K4me3. The extracted DNA was size-selected with AMPure XP and E-gel electrophoresis system. A DNA library was prepared with a ThruPLEX DNA-Seq kit (TAKARA). RNA-seq and ChIP-seq libraries were prepared for sequencing using standard protocols.