GSM7157326: 39mpi,16-Sep-2011,V42,GEX,biol rep 6; Homo sapiens; RNA-Seq
Memory B cells separated from approximately 1x106 PBMCs by selective depletion (Switched memory B cell isolation kit; Miltenyi Biotec) were loaded according to the 10X Genomics manufacturer's protocol for 5'-gene expression to attain 10,000 sequenced cells per well. Gene expression libraries were prepared according to 10x genomics manufacturer's protocol.