GSM8253609: AND2 hCOs 45 days scRNAseq; Homo sapiens; RNA-Seq
hCOs were collected with the aid of a pipette and treated with HBSS (Hank's Balanced Salt Solution; 1X, Gibco, #14180046) for 15 minutes. The organoid were centrifuged (3 minutes at 600 rpm) and digested with trypsin (0.025%, Gibco) for 20 minutes at 37°C and trypsin inhibition with 1:1 DMEM:F12 + 10% FBS. Subsequently, the dissociated and individual cells were centrifuged (3 minutes at 600 rpm) and resuspended in a buffered solution of BSA (0.04%, Sigma) in PBS at 4°C. The single cell library was prepared according to manufacturer's instructions using the 10X Genomics Chromium Single Cell 3' Library & Gel Bead Kit v3.1 (10X Genomics) workflow by the Genomics Unit of the Instituto de Salud Carlos III