GSM7036228: s1_scr; Rattus norvegicus; RNA-Seq
Total RNA was isolated used the Rneasy kit from Qiagen, according to the manufacturer's protocol. RNA sequencing was performed on four biological replicate samples per group by Novogene (Sacramento, CA). Messenger RNA was purified from total RNA using poly-T oligo-attached beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP. The directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification and purification.