GSM7230939: WT1_biol_rep1; Danio rerio; RNA-Seq
The RNA of single ventricles (one female and one male per condition) was isolated using the RNeasy Micro Kit (Qiagen), according to the manufacturer's instructions.  Trimmed reads were aligned against the Ensembl zebrafish genome version danRer11 (ensembl release 104) using STAR 2.7.9a with the parameters “--outFilterMismatchNoverLmax 0.1 --alignIntronMax 200000” 1µg of total RNA was used as input for VAHTS Stranded mRNA-seq Library preparation following manufacture's protocol (Vazyme).