Spider venom gland and venom symbiotic bacteria
1) Add the official Illumina connector sequence to the outer end of the target region through PCR;2) Use gel recovery kit to cut and recover PCR products;3) Tris HCl buffer elution and 2% agarose electrophoresis detection;4) Sodium hydroxide denaturation produces single stranded DNA fragments.Reagent: TruSeqTM DNA Sample Prep Kit