GSM7518658: RPK_tumor_AAV_1 technical rep2; Mus musculus; RNA-Seq
liver samples and liver tumors were isolated using the Maxwell 16 LEV simplyRNA Kit (Promega) according to the manufacturer's instructions Reverse transcription was carried out for individual samples using the Maxima RT polymerase with oligo-dT primers consisting of a sample barcode, unique molecular identifiers (UMIs) and an adaptor. Ends of cDNAs were extended by a template switch oligo (TSO) and full-length cDNA was amplified with primers binding to the TSO-site and the adaptor. For fragmentation of cDNA, NEB Ultra II FS kit was used, followed by end repair and A-tailing, to which a TruSeq adapter was ligated. Finally, 3'-end-fragments were amplified using primers with Illumina P5 and P7 overhangs