GSM6481886: DD PHF8WT replicate 2; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a focused-ultrasonicator S2 Covaris. One ng per sample of fragmented and purified DNA as starting material was used. The size of the DNA fragments was checked on the Agilent TapeStation and an average of fragments between 300-400 base pairs was obtained.