RNA-seq of HCT116 samples processed with TREX for U1 interactome detection (no RNAseH control)
Libraries for RNA sequencing were prepared from 700 ng of purified RNA from samples not subjected to RNAseH treatment, using Lexogen RiboCop rRNA Depletion HMR V2 kit for ribosomalRNA depletion coupled to CORALL Total RNA-seq V2 kit. Lexogen UDI 12 nt was used asindexing system, and the PCR Add-on Kit for Illumina was used to optimize the number of PCRcycles. The PCR-amplified indexed libraries were sequenced to a depth of 40 million reads persample, using 150bp paired end sequencing on an Illumina NovaSeq 6000 instrument (NovogeneUK Company Limited, Cambridge).