RNA-Seq of Rattus norvegicus:Adult male Amygdala
Amygdala tissue samples from adult rat in utero exposed to cannabis and  smoke-free controls were quickly dissected and flash frozen in liquid nitrogen for gene expression analysis.Total RNA was extracted from the amygdala of adult rats using the MagMAX mirVana Total RNA Isolation kit (Thermo Fisher). RNA quality was assessed using the Qubit RNA BR Assay (ThermoFisher) and RNA Screentape (Agilent). Sequencing libraries were constructed from 300 ng of RNA per sample using the TruSeq Stranded mRNA Library Prep kit (Illumina).  Sequencing libraries were evaluated using the Qubit dsDNA BR Assay (ThermoFisher) and a D1000 Screentape (Agilent). The barcoded libraries were pooled equimolar and 75 bp paired-end reads were generated on a NextSeq 550 instrument (Illumina).