Native Nanopore SMS of human stool microbiome
Library preparation was performed using the SQK-RBK114-24 kit according to Nanopore recommendations. The prepared libraries were loaded onto R10.4.1 flow cells and sequenced on a MinION Mk1B instrument. The sequencing time was set to 48 h with a minimum read length of 200 bp, read filtering equal to a Q-score of 9, and read splitting "ON". Data acquisition was performed with MinKNOW v23.04.5 and basecalling with Guppy v6.5.7 using the high accuracy setting (400 bps). After sequencing, the data was preprocessed to remove adapters and decontaminated from human reads.