GSM7157634: siControl_3; Rattus norvegicus; RNA-Seq
Total RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent RNAScreenTape. 0.5 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using NEBNext® Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit following manufacturer's protocols.