GSM7886107: YY1aid_async_untreated_RAD21_ChIPseq_Rep1; Mus musculus; ChIP-Seq
For ChIP-seq, DNA from lysed cells was sonicated, immunoprecipitated, and then extracted using the QIAquick PCR Purification kit (Qiagen 28106). For Micro-C, DNA was digested with MNase, proximity-ligated, and extracted using phenol:chloroform:isoamyl alcohol extraction and ethanol precipitation. Gel extraction was used to select for fragments between 250-400bp. DNA was purified with Zymoclean Gel DNA Recovery kit (D4007). We used the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB E7635) with unique dual-indexed primers (NEB E6440).