16S of Gadus morhua: gut microbiome
Gut microbiome content (180-220 mg) was extracted for total DNA using a QIAmp Fast DNA Stool Mini Kit (Qiagen), with a total of 4 treatments at each time point in control and oiled juvenile Atlantic cod. The TruSeq Nano DNA kit was used, with amplicons prepared with the V4-V5-primers: 799Fmod3 (5-CMGGATTAGATACCCKGG- 3) and 1115R (5-AGGGTTGCGCTCGTTG -3). An Agilent Bioanalyzer Chip was used for the quality control of libraries. Samples were sequences on an Illumina MiSeq platform (2 x 300 bp paired end). Sequencing was performed by the University of California, Riverside Genomics Core.