GSM7518479: Skin, excised, cultured, expanded, for myogenic induction, Rep3; Homo sapiens; RNA-Seq
Total RNA was extracted with TRIzol (Thermo Fisher Scientific), eluted in 20 µl RNase-free water and concentration estimated by A260. RNA integrity number was assessed with an Agilent 2100 Bioanalyzer using the Agilent RNA 6000 Nano kit Libraries were prepared using the KAPA mRNA HyperPrep kit (Roche)