GSM8207511: Amniotic fluid cells, control pregnancy 1; Rattus norvegicus; RNA-Seq
Total RNA was extracted using Trizol reagent (thermofisher, 15596018) following the manufacturer's procedure. After passing the quality inspection of the total RNA, magnetic beads connected to Oligo (dT) were used to enrich eukaryotic mRNA (for prokaryotes, rRNA was removed using a reagent kit to enrich mRNA), and then Fragmentation Buffer was added to randomly break the mRNA into short fragments. Using fragmented mRNA as a template, synthesize a single strand of cDNA using six base random primers, and then connect an adapter with Unique Molecular Identifiers (UMI); Finally, PCR amplification was performed to obtain the final sequencing library.