GSM7230928: A2780-Taxol,paclitaxel resistant; Homo sapiens; RNA-Seq
RNA was harvested using Trizol method, mRNA molecules were purified from total RNA using oligo(dT)-attached magnetic beads for the construction of sequencing libraries. mRNA molecules fragmented and reversed transcription into cDNA. The synthesized cDNA was subjected to end-repair and added 'A' base and Adapter connection, PCR amplification.  Library was validated on the Agilent Technologies 2100 bioanalyzer.PCR products were heat denatured and circularized. The single strand circle DNA  were formatted as the final library.