WGS of homo sapiens:bone marrow
100ng of genomic DNA for a 350 bp insert size was fragmented using a Covaris S2 Ultrasonicator. DNA sequencing libraries were constructed using the TruSeq Nano DNA Sample Preparation Kit from Illumina (San Diego, CA) according to the manufacturer protocol. The quality of amplified libraries were confirmed by electrophoresis on Agilent Bioanalyzer High Sensitivity DNA Kit (part # 5067-4626) (Agilent, CA). Libraries were quantified using the KAPA Library Quantification Kit (KK4824) ( Kapa Biosystems, MA) according to the manufacturers library quantification protocol. Following cluster amplification of denatured templates, sequencing was progressed as paired-end (2150bp) using Illumina Novaseq6008 (Illumina, CA, USA).