GSM7616079: Brain 3; Mus musculus; OTHER
Genomic DNA was extracted from each tissue using the Gentra Puregene tissue kit (Qiagen #158689) and DNA pellets were resuspended in nuclease-free water.  DNA was phenol:chloroform extracted and ethanol precipitated before proceeding sgRNA were amplified from DNA extracts from lung or brain tissue.  Amplicons were then amplified with a mix p5 (including stagger for diversity) and uniquely barcoded p7 primers to add illumina adapters to each sample amplicon sequencing