GSM8119410: D0-C16; Homo sapiens; RNA-Seq
hiPSCs were dissociated using Accutase and counted to load 10,000 cells into on channel of a 10X Chromium chip. One channel per sample was used. After emulsion cell lysis and RNA was extracted followed by library preparation. Libraries were sent to Novogene for sequencing. Single cell suspensions were passed through 40μm cell strainer (Corning) and concentration was adjusted to 1000 cells/μL. Suspensions were loaded in single-cell-G Chip (10X Genomics) for target output of 10,000 cells per sample. Single-cell droplet capture was performed on the Chromium Controller (10X Genomics). cDNA library preparation was performed in accordance with the Single-Cell 3' v 3.0 or v3.1 protocol. Libraries were evaluated for fragment size and concentration using Agilent HSD5000 ScreenTape System (Agilent).