GSM7794256: iTregs IP(H3R2me2s); Mus musculus; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes at room tenmperature, then washed with 5 M glycine. Cells were then lysed and sonicated in biorupter pico sonicator system. The high-throughput DNA sequencing libraries were prepared by using VAHTS Universal DNA Library Prep Kit for Illumina V3(Catalog NO. ND607, Vazyme). The library products corresponding to 200-500 bps were enriched, quantified and finally sequenced on Novaseq 6000 sequencer (Illumina) with PE150 model.