RNA-seq of Homo sapiens;Fresh frozen: Sample 9830-020185A-R0i_CP-3046
Tumor tissue was disrupted using roto-stator homogenization. RNA was isolated using the RNeasy Mini Kit (QUIAGEN) according to manufacturer protocol. RNA was quantified using a NanoDrop® Spectrophotometer, and the quality of RNA was then analyzed via 2100 Bioanalyzer Instrumentwhich using the RNA 6000 Nano assay (Agilent) for determination of an RNA Integrity Number (RIN). Briefly, 1 µg of total RNA was converted to RNAseq libraries using the TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold (Illumina) and sequenced on an Illumina HiSeq 2000 or HiSeq2500 using a 2x50bp configuration