Transcriptome of sacccharomyces cerevisiae MU310
Firstly, using the structural feature that most eukaryotic mRNAs have polyA tails, mRNAs with polyA tails were enriched by Oligo(dT) magnetic beads. secondly, mRNAs were obtained by removing ribosomal RNAs from total RNAs. mRNAs were subsequently randomly interrupted by divalent cations in NEB Fragmentation Buffer, and the mRNAs were then used to construct libraries according to the NEB common library construction method or strand-specific library construction method. The libraries were constructed according to the NEB common library construction method or strand-specific library construction method  Translated with www.DeepL.com/Translator (free version)