Miseq of Rhizobium leguminosarum sv. trifolii: rpoB, recA, nodA and nodD amplicons from Store Heddinge T. repens nodules.
DNA was isolated from T. repens nodules collected from Store Heddinge, Denmark. Individual PCRs using Platinum (non-proofreading) polymerase for rpoB, recA, nodA and nodD Rlt genes were pooled for each sample.