Nanopore sequencing of Fusarium oxysporum f. sp. spinaciae: isolate Fus167
Isolated high-molecular weight DNA from frozen mycelium, size-selected with NaCl/PEG precipitation. Prepared the library with Oxford Nanopore's Rapid Sequencing Kit (RAD004). Prepared a library with >5 ug DNA and 1.5 ul FRA buffer. No other modifications to ONT protocols were performed. Sequenced the library on the MinION with an R9.4.1 flow cell following the manufacturer's protocol.