GSM6754156: Rbbp4-AID 24h_KAP1_rep1; Mus musculus; OTHER
CUT&RUN assay was done as described in Meers et al (2019) with slight modifications. 1 × 105 cells were harvested and bound to Concanavalin A-coated beads, then incubated with individual primary antibody at 4°C overnight. pAG-MNase enzyme was added to each sample followed by incubation 1 hour at 4°C. Targeted digestion was done by 30 minutes incubation on ice block (0°C) under low salt conditions.The DNA released into the supernatant were purified and subjected to library preparation and next-generation sequencing. The sequencing libraries were prepared through VAHTS Universal DNA Library Prep Kit (Vazyme, ND607).