RNA-seq of Dermacentor variabilis adult salivary glands during feeding on rabbits
Intact salivary glands (SG) from unfed Dermacentor variabilis adult males were collected and stored in RNAlater solution. RNA extraction was performed with the AllPrep DNA/RNA/Protein Mini Kit (QIAGEN) according to manufacturer`s specifications and analyzed with an TapeStation system (Agilent, USA). The library was constructed using the NEBNextUltraTM II (Directional) RNA with polyA selection library prep kit and sequencing was performed in an Illumina Novaseq 6000 DNA sequencer.