GSM6858851: iAT1 3D; Homo sapiens; RNA-Seq
Cells were dissociated to single cell using 0.05% trypsin for 3D samples and Accutase for ALI samples. Each were sorted for live cells using DRAQ7. Single cells were captured for sequencing library preparation using a 10X Chromium (10X Genomics, Pleasanton, CA) instrument at the BUMC Single Cell Core. Single-cell RNA-seq libraries were prepared according to the Single Cell 3' v2 Reagent Kits User Guide (10X Genomics). Sequence libraries were constructed using the Chromium Single-Cell 3' Library Kit (10X Genomics). Sequencing libraries were loaded on a NextSeq500 (Illumina) to obtain a sequencing depth of~50K reads per cell. 685 cells from CKDCI 3D, 1340 cells from LDCI 3D, 643 cells from LDCI ALI p0, and 1093 cells from LDCI ALI p1 were captured and sequenced.