Low-coverage whole genome sequencing of Dioscorea spongiosa
Genomic DNA was extracted from silica gel-dried leaves using DNAsecure Plant Kit (Tiangen Biotech, Beijing, China), following the manufacturers protocol. DNA concentration and integrity were measured by Agilent 2100 BioAnalyzer and agarose gel electrophoresis. Paired-end library with insert size of 350 bp was constructed for each species/subspecies by using the Genomic DNA Sample Prep, and then sequenced on the Illumina HiSeqTM 4000 platform (Illumina, San Diego, California, USA) according to the paired-end 2  150 bp protocol. Library construction, genome sequencing and raw data filtering were conducted by Novogene Bioinformatics Technology Co., Ltd., Beijing, China.