Study of Spp1 Knock out in brain macrophages compare to wild type mice
Study of Spp1 Knock out in brain macrophages compare to wild type mice
Embryo at E14.5 and E18.5 were sacrificed and brain was collected, cut in tiny pieces and digested with collagenase and DNAse in RPMI. CD45+ cells were then enriched using CD45 microbeads from myltenyi and the possel function of the automacs. CD45+ cells were then counted and viability was determined using BD Rhapsody. Each sample contain 6 barcoded samples using BD Rhapsody multiplexing kit. 6 Sample tags were used for Sample 1 corresponding to E14.5 embryos: SMK#4, 5 and 6 correspond to Spp1 KO, SMK#10, 11 and 12 correspond to Wild type. 6 Sample tags were used for Sample 2 corresponding to E18.5 embryos: SMK#7, 8 and 9 correspond to Wild type, SMK#10, 11 and 12 correspond to Spp1 KO.  Cells were then loaded in BD Rhapsody cartridge.  Using Single-Cell Capture and cDNASynthesis with the BD Rhapsody™ Express Single-Cell Analysis System and protocol Using Single-Cell Capture and cDNASynthesis with the BD Rhapsody™ Express Single-Cell Analysis System and protocol