GSM8119089: Light-seq E14 mGFP hindgut 4; Gallus gallus; RNA-Seq
in situ reverse transcription was used to generate cDNA from tissue sections on slides, which was displaced from RNA via incubation for 45min in a solution containing RNaseH (NEB) and 10X Thermopol buffer RNA-seq libraries were constructed using the Nextera XT Library Preparation Kit, following the manufacturer's protocol