Whole-genome Pacbio HiFi Parent Washington Navel
Mature leaf tissue was collected from a single Parent Washington Navel orange accession at the University of California, Riverside Givaudan Citrus Variety Collection in Riverside, CA. High molecular weight DNA was isolated using the protocol Preparing Arabidopsis Genomic DNA for Size-Selected ~20 kb SMRTbellTM Libraries with the addition of a sorbitol wash step following nuclei isolation. The library was constructed and sequenced using the Pacific Biosciences Sequel II platform by the DNA Technologies and Expression Analysis Core at the UC Davis Genome Center.