Pacbio_HiFi:S.bicolor
DNA was extracted using the MagAttract HWM DNA kit from Qiagen (Hilden, Germany). The DNA was fragmented to 15-20 kb fragments using Megaruptor 3, and libraries were prepared using Pacific Biosciences protocol for HiFi library prep using SMRTbell ExpressTemplate Prep Kit 3.0. Final library was size selected using BluePippin with a 10kb cut-off. Each library was sequenced on three 8M SMRT cells on Sequel II instrument using Sequel II Binding kit 2.2 and Sequencing chemistry v2.0. Loading was performed by adaptive loading, movie time: 30 hours, pre-extension: 2 hours.