PCRseq of Danio rerio: CRISPR/Cas9 mediates sox9a gene editing the testis-1 of F0 generation zebrafis
To assess the efficiency of gene editing in RNP-based systems, we performed deep sequencing of the target sites using Illumina high-throughput sequencing platform. Tissue DNA from sox9a gene editing in zebrafish F0 generation individual fish was extracted and subjected to PCR amplification and amplicon library was constructed for each target.