GSM6858856: L+DCI; Homo sapiens; RNA-Seq
Cells were dissociated to single cell using 0.05% trypsin and sorted for live cells using DRAQ7. Single cells were captured for sequencing library preparation using a 10X Chromium (10X Genomics, Pleasanton, CA) instrument at the BUMC Single Cell Core. Single-cell RNA-seq libraries were prepared according to the Single Cell 3' v2 Reagent Kits User Guide (10X Genomics). Sequence libraries were constructed using the Chromium Single-Cell 3' Library Kit (10X Genomics). Sequencing libraries were loaded on a NextSeq500 (Illumina) to obtain a sequencing depth of~50K reads per cell. 623 iAT2s in CKDCI, 1212 cells from the YAP5SA in CKDCI condition and 1539 iAT1s in LDCI were captured and sequenced.