GSM8254172: Myofiber REST-cKO 1; Mus musculus; RNA-Seq
Muscle Stem Cells (ITGA7+/VCAM1+/CD11b-/CD45-/CD31-/Ly6a-) were isolated by Fluorescence Activated Cell Sorting (FACS) into SMART lysis buffer. EDL muscle was dissected and myofibers were isolated as previously described (Blackburn, D.M. et al. Journal of Biological Chemistry, 2019). After isolation and selection of a single myofiber, myofibers were lysed SMART lysis buffer and libraries generated as previously described (Blackburn, D.M. et al. Journal of Biological Chemistry, 2019). RNA-Seq libraries were made using SMART-Seq for the conversion of cDNA followed by Nextera XT for incorporation of Illumina sequencing.