DNA sequence of human:adult male bronchial
Tissue DNA was sheared using CovarisM220, followed by end repair, phosphorylation, and adaptor ligation. Fragments of size 200-400bp were selected by bead (Agencourt AMPure XP Kit, Beckman Coulter, Brea,CA, USA), followed by hybridization with capture probes baits, hybrid selection with magnetic beads and PCR amplification.A bioanalyzer high-sensitivity DNA assay was performed to assess the quality and size of DNA.