GSM7996413: YS-E11-LB-WT1; Escherichia coli K1; RNA-Seq
At OD=0.600, the bacteria were harvested and washed by centrifugation, then recovered in lysis buffer from the RNA extraction kit, Bioanalysis (Valencienner, Germany) reference 740955.50. Total RNA from both strains was extracted using this kit according to the manufacturer's instructions. RNA concentration was quantified using a spectrophotometer (Nanodrop Technologies). RNA-Seq analysis was carried out by the Genotyping/Sequencing Platform of the Brain Institute - ICM, France. mRNA library preparation was realized following manufacturer's recommendations (Stranded Total RNA Prep with Ribo-Zero Plus Kit from ILLUMINA). Final samples pooled library prep were sequenced on Nextseq 2000 ILLUMINA P2-200 cartridge (2x400Millions of 100 bases reads) corresponding to 2x14Millions of reads per sample after demultiplexing