GSM7231176: 22_16; Mus musculus; RNA-Seq
Single cell suspensions were processed through 10X Genomics Chromium controller using the Single Cell Gene Expression kit (10X Genomics, Chromium Next GEM Single Cell 3' Kit v3.1) to generate emulsions which were first reverse transcribed and then PCR amplified to generate cDNA. Sequencing libraries were then generated using 10µl of cDNA as outlined in the 10X Genomics protocol (CG000315 Rev C). Briefly, cDNA was first fragmented, end repaired and adaptors ligated, followed by PCR amplification and size selection to generate final libraries. Libraries were sequenced on a NovaSeq S4 flowcell (Illumina) to a depth of 25,000 reads per cell.