RNA-Seq of APCs
TheRNAqualitywasconfirmedthroughAgilent2100,and5gperRNAsamplewasusedtoconstructthelibrary.ThetaggedcDNAlibrarieswereloadedontotheBGIseq500platform(BGI-Shenzhen,China)andsequencedforsingle-end50basesread.Thereadsthatcontainedthesequencingadaptorandunknownbases(>5%"N"sperread)andthelow-qualityreads(>15%basessmallerthanQ20perread)werediscarded.Theselectedcleanreadsfromeachsamplewerealignedandmappedtothesikadeer(CervusNippon)referencegenomeusingBowtie(v2.2.5,http://bowtie-bio.sourceforge.net/Bowtie2/index.shtml).