GSM7916761: E_BI09_BCR; Homo sapiens; RNA-Seq
16,000 cells per reaction, gel beads and partitioning oil were loaded to Chromium Next GEM Chip K for gel GEM generation and barcoding on Chromium Controller (10X genomics). The GEMs were transferred to prepare GEM-RT incubation. According to chromium next GEM single cell 5' v2 user guide(10X genomics), 50ng cDNA per sample was used to construct 5ʹ Gene Expression (GEX) and V(D)J library