scRNA-seq of tissues from liver cancer patients
 Cryopreserved samples were thawed and prepared according to the Single Cell 5' Reagent Kits User Guide. Specifically, the samples were washed and re-suspended in PBS + 0.04% BSA. Cell viability was determined. The cDNAs was obtained after the GEM generation and barcoding, followed by GEM RT reaction. Purified cDNA was amplified for 14 cycles. A clean up using SPRIselect beads was performed. cDNA libraries were prepared with 10x Genomics Single Cell 5' library & gel bead kit v1.1. cDNA concentration was determined by Bioanalyzer.