RNA-seq of Drosophila melanogaster: apical larval midgut
Quality of total RNA was evaluated using the Agilent 2100 Bioanalyzer.Poly-A purification of RNA was performed using the NEBNext Poly(A) mRNA MagneticIsolation Module and library construction completed with the KAPA Stranded RNA-SeqLibrary Preparation Kit. Sequencing was carried out on a HiSeq 2500 (Illumina) in 75-bp,single-end sequencing reactions.