GSM7518447: MSC,3D cultured,rep2; Homo sapiens; RNA-Seq
Total RNA was extracted from 2D and 3D cultured MSCs using the RNeasy kit (Qiagen, Hilden, Germany) according to the manufacturer's instruction and quantified with UV/VIS Nano spectrophotometer (Nabi, MicroDigital Co.,Ltd., Bundang-gu, Seongnam-si, Gyeonggi-do, Korea). Reverse transcription of RNA was performed using a Maxima First Strand cDNA Synthesis Kit (Thermo Fisher Scientific). The construction of RNA-sequencing library was performed using Truseq RNA Sample preparation kit v2 (Illumina, Inc., San Diego, CA, USA, RS-122-2002).