Amplicon sequence of vermicompost: Jaipur, Rajsthan
Genome was isolated using CTAB protocol. DNA integrity was checked using Agarose Gel Electrophoresis with 0.8% Agarose Gel and concentrations were determined by using Qubit dsDNA BR Assay Kit (Catalog: Q32853, Thermo Fisher Scientific), which contains dye as well as buffer and two standards. The DNA was amplified using primers specific to V3-V4 regions. The PCR product was checked on Agarose Gel to confirm amplification. The amplified product should be ~550bp.  The library concentration was determined in a Qubit 4 Fluorometer (Life technologies) using the Qubit 1X dsDNA HS Assay Kits (Catalog: Q33231, ThermoFisher Scientific). The library quality assessment was done using Agilent D1000 ScreenTape System in a 2200 TapeStation System (Agilent) which is designed for analysing DNA molecules from 25 to 1000bp.