GSM6858572: chmods_SppsG8810_16_18hrs_R1; Drosophila melanogaster; RNA-Seq
Eggs from mutant flies grown in heterozygosis with GFP-marked balancer chromosomes were collected for two hours on agar plates and aged for the appropriate amount of time at 25°C. Embryos were dechorionated following standard procedures and placed on a plate containing halocarbon oil. 20 to 30 mutant embryos were selected according to morphology and against GFP signal and homogenized prior to Trizol extraction.