GSM7994747: Crohn's disease; Homo sapiens; RNA-Seq
FACS was performed to isolate α4+ memory T cells for subsequent scRNA-seq, with α4+ memory T cells being defined as CD3+CD45RA-α4+ viable single lymphocytes. Single cell suspensions obtained from FACS were loaded in the 10x Chromium Controller and cDNA single-cell library was constructed using the dual index Chromium Single Cell 3' v3.1 protocol of 10x Genomics, Inc.