GSM8207649: Flag_MBD2c_input; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde and subsequently quenched with 0.125 M glycine. The cells were sonicated using a Scientz JY99-IIDN device. Anti-Flag antibody (Sigma-Aldrich) were used. The DNA fragments were end-repaired and A-tailed, which were further ligated with sequencing adaptors. The final DNA library was obtained after size selection and PCR amplification. Libraries were analyzed for size distribution by Agilent 5400 system(Agilent, USA) and quantified by qPCR (1.5 nM).