GSM6754173: Rbbp4-AID 24h_MNase_rep1; Mus musculus; MNase-Seq
1 x 106 cells were harvested and transferred into lysis buffer. Nuclei were then pelleted, washed and resuspended in digestion buffer containing 2 mM CaCl2 and 2,500 gel units micrococcal nuclease (NEB, M0247S) with shaking at 37℃ for 5 min.  The reaction was stopped by EDTA and protease was added to digest proteins. Then fragmented and naked genomic DNA were released. The sequencing libraries were prepared from 10 ng of purified mono-nucleosome-DNAs using the VAHTS® Universal DNA Library Prep Kit (Vazyme, ND607) with 10 cycles of PCR.