RNA-seq of HCT116 samples processed with TREX for U1 interactome detection (RNAseH +)
Libraries for RNA sequencing were prepared from 700 ng of purified RNA from RNase H treatedsamples, using Lexogen RiboCop rRNA Depletion HMR V2 kit for ribosomalRNA depletion coupled to CORALL Total RNA-seq V2 kit. Lexogen UDI 12 nt was used asindexing system, and the PCR Add-on Kit for Illumina was used to optimize the number of PCRcycles. The PCR-amplified indexed libraries were sequenced to a depth of 40 million reads persample, using 150bp paired end sequencing on an Illumina NovaSeq 6000 instrument (NovogeneUK Company Limited, Cambridge).