GSM7793738: PKM2-/- heart, 3d MI 2; Mus musculus; RNA-Seq
RNA was extracted from mouse left ventricle tissue using the Rneasy mini kit (Qiagen). 500ng of RNA was used for the construction of sequencing libraries. rRNA was removed using the rRNA depletion kit v2 (New England Biolabs). cDNA libraries for sequencing was prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs) with index primers NEBNext Multiplex Oligos for Illumina sets 1 and 2 (New England Biolabs).