GSM8255337: In Vitro SreA transcript, No SAM, 1M7; Listeria monocytogenes; OTHER
RNAs were buffer exchanged by centrifugation filter before library preparation RNAs were ligated to a 3' end adapter including an RT primer binding site. cDNA was generated by MaP RT with Superscript II including a template-switching oligonucleotide. Final sequencing libraries were generated by PCR using primers complentary to 3' adapter and template-switching oligo sequences, with additional indexing barcodes included.