GSM7518334: Shade, Input, rep2; Arabidopsis; ChIP-Seq
Seedings were fixed with 1% formaldehyde for 15 minutes under vacuum condition. After grind and lysis, chromatin was sonicated using a Bioruptor Next Gen UCD-300 (Diagenode). ChIP-seq libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645S). Amplified libraries were size selected using 2% gel to capture fragments between 100 and 400 bp. Libraries were quantiﬁed using the Agilent 2100 Bioanalyzer (Agilent Technologies). Samples without antibodies were used as the input control. The sample library was sequenced on NovaSeq6000 PE150.