Gryllus longicercus genome sequencing and assembly
Insect tissues excluding gut were dissected and placed in tubes containing DNA/RNA shield and incubated before being further mechanically broken down with pestles. To induce digestion Solid Tissue Buffer and Proteinase K were added. After incubation the remaining tissue fragments were broken down with a squisher. The lysate was then spun down and the supernatant was purified using Quick-DNA HMW MagBead Kit. Elutes from each tube were pooled together to produce the final purified HMW DNA. Purified genomic DNA was prepared following the NEB Ultra II kit and sequenced with Illumina NovaSeq 6000 instrument.