GSM7519148: HeLa_OEKDM5C, BRD4; Homo sapiens; ChIP-Seq
Cells were fixed in 1% formaldehyde for 10min and then quenched with 0.125M glycine for 10min at room temperature with rotation. Cell pellet was lysed with cell lysis buffer (10mM Tris, pH8.0; 10mM NaCl; 0.2% NP-40; protease inhibitor) on ice for 10min and chromatin DNA was sonicated to 200-500bp average in size. Chromatin fragments lysates were immune-precipitated with primary antibodies at 4°C overnight and then conjugated with protein A/G Beads 4FF (Smart-lifesciences, SA032500). Bound fractions were washed and eluted from agarose beads. ChIP and input samples were de-crosslinked at 55°C overnight. ChIP-seq libraries were prepared using the NEBNext ultra II DNA library prep kit for Illumina.