Bacterial diversity of Peltigera fuscopraetextata thallus COY13-049
PCR of V4-V5 region of bacterial 16S was amplified with primers that exclude cyanobacteria: 515F (Turner et al., 1999; 5-CCCCGTCAATTCCTTTGAGTTTYAR-3) and 904RM (modified from Hodkinson & Lutzoni 2009; 5- CCCCGTCAATTCCTTTGAGTTTYAR -3). Band of interest was purified from agarose gels and sended to Macrogen Inc. (Korea) for Illumina MiSeq 300bp x 2 amplicon sequencing.