GSM8254775: JAK2V617F mutant cardiac EC following high-fat diet treatment; Mus musculus; RNA-Seq
Total RNA was extracted using the RNeasy mini kit (Qiagen, Hilden, Germany) according to manufacturer protocol. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads and used to generate sequencing libraries using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (New England BioLabs, MA, USA).The library preparations were sequenced on an Illumina platform. Index of the reference genome was built using Hisat2 v2.0.5 and paired-end clean reads were aligned to the reference genome.