GSM7035859: ST29_biol rep 3; Staphylococcus aureus; RNA-Seq
To quench cellular metabolism / transcription and to stabilize RNAs, cells were harvested by centrifugation at 10,000 g for 1 min at 4°C and the pellets immediately frozen in liquid nitrogen before storage at −80°C. For extraction of RNA, cells were lysed using Lysing Matrix B and a FastPrep instrument (both MP Biomedicals), and RNA were isolated using the RNeasy Mini Kit (Qiagen). RNA libraries for RNA-seq were prepared using TruSeq Total RNA Stranded Library Prep Kit following manufacturer's protocols.