GSM7518690: a4b7+ Eos sample 2; Mus musculus; RNA-Seq
The cells were lysed in Tri Reagent (Sigma-Aldrich) and processed according to the manufacturer's protocol until the phase separation. The aqueous phase was collected and RNA was extracted with a Direct-zol RNA Microprep Kit (Zymo Research) according to the manufacturer's instructions, including the DNase step. Sequencing libraries were constructed with SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Takara Bio) according to the manufacturer's instructions. 3 ng of total RNA was used as input.