GSM7518582: RNA191 Endometrium, young maternal age; Homo sapiens; RNA-Seq
Total RNA was extracted from all samples using miRNeasy kit (Qiagen, cat.no. 217084), followed by DNase treatment (DNase Free, Life Technologies, cat.no. EN0521), RNA integrity was assessed with Qubit RNA IQ Assay kit (Thermo Fisher, cat.no. Q33221) cDNA libraries were randomly assigned into two sequencing batches  and prepared using the TruSeq Stranded mRNA Sample Preparation Guide (Illumina, cat.no. 20020595).