GSM7696825: L929sAhFas, Untreated X01-UT, E373 2; Mus musculus; OTHER
Total RNA was extracted from cells or purified EVs using the TRI reagent (ThermoFisher, ARNA was extracted from purified EVs using the TRI reagent (ThermoFisher, AM9738), according to the manufacturer's instructions and hydrolyzed in bicarbonate buffer. The reaction was stopped by ethanol precipitation. The resulting fragments were then end-repaired and converted into an Illumina library using the NEBNext Small RNA Library Prep kit (Bioke, E7300S) and sequenced at the ULB brightcore facility.