GSM7233583: preleptotene-enriched testes, wild-type, biological replicate 2; Mus musculus; RNA-Seq
Total RNA was extracted from 4 wild-type samples and 3 Meioc-null samples, using 1.5 synchronized testes from a single pup per sample. TRIzol Reagent (Thermo Fisher Scientific) was added to freshly thawed whole testes, and ERCC RNA ExFold RNA Spike-In Mix 1 and 2 (Thermo Fisher Scientific) were added to wild-type and Meioc-null samples, respectively, at a concentration of 1 l of a 1:100 dilution of spike-in mix per 1 mg of testis tissue. (Spike-in mixes were ultimately not used for data analysis.) Total RNA was then isolated with chloroform following the manufacturer's protocol, precipitated via isopropanol, and resuspended in RNase-free water RNA-seq libraries were prepared with the TruSeq Stranded Total RNA kit with the Ribo-Zero Gold rRNA Removal kit following manufacturer's protocols.