Nanopore reads of Fusarium poae Fp133 (DAOMC 252341)
gDNA was isolated from 500mg of frozen tissue using Illustra Phytopure Genomic DNA Extraction kit.  6ug gDNA was size fractionated with a 0.75% agarose gel cassette (10 kb  40 kb) using a SageELF instrument, and the three most abundant fractions were combined for Nanopore sequencing selecting for long reads using a slightly modified revC protocol, wherein beads were reused after each elution, and incubation times increased to 10 min. The Long Fragment Buffer was used in the adapter ligation step before running on a MinION for 48 hours.