RNA-seq of turbot: control IM, brain sample
RNA from the different samples was isolated using the Maxwell RSC simplyRNA Tissue Kit (Promega) with an automated Maxwell RSC 48 Instrument in accordance with the instructions provided by the manufacturer. The quantity of RNA was measured on a Qubit 4 fluorometer (Thermo Fisher Scientific), and RNA integrity was analysed in an Agilent 2100 Bioanalyzer (Agilent Technologies Inc., Santa Clara, CA, USA) according to the manufacturer's instructions.