GSM8436485: P301S_hom_CD8_WT_33weeks; Mus musculus; RNA-Seq
Two mice per experimental group were collected and perfused with chilled normal saline. Spinal cords from each animal were dissected and two spinal cords were combined per tube for processing. Tissues were then processed using the Miltenyi Tumor Dissociation Kit (#130-096-730, Miltenyi Biotec) and the gentleMACS™ Octo Dissociator with Heaters on the soft/medium program (37C_m_TDK_1). Following dissociation, myelin was removed using Myelin Removal Beads II (#130-096-731, Mitenyi Biotec) according to the manufacturer's.  protocol. single cell suspension quality, number and viability were assessed with the dual fluorescence cell counter Luna-FX7 (Logos Bio). Gel Bead-in-Emulsions were prepared by loading up to 10,000 cells per sample onto the 10X Genomics Chromium Next GEM Chip G (cat#1000127). Barcoded single cell cDNAs and DNA libraries were generated with Chromium Next GEM Single Cell 3' Kit v3.1 (10x Genomics; cat#1000269) following the manufacturer's standard protocol