GSM8330881: Huh-7 cells, Biological replicate 2; Homo sapiens; RNA-Seq
Total RNA was isolated using Trizol reagent (Thermo Fisher Scientific, # 15596018) following manufacturers protocol. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dTTP. It was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.