RNA-seq of mus musculus: PDAC-B
Total RNA extraction and purification from cells was performed using the RNeasy Mini Kit (Qiagen) combined with RNaseFree DNase (Qiagen) treatment, following the manufacturer's instructions. Measurements of RNA concentration and purity were routinely performed using a NanoDrop 2000C (Thermo Fisher Scientific) before downstream processing.  Libraries were prepared using a Library Prep kit (Illumina TruSeq RNA Sample prep kit v2) and were run on an Illumina NextSeq500 platform using a High Output v2 75 cycles (236 cycle, paired end, single index) sequencing kit.  For full Methods see Papalazarou, Drew et al.J Cell Sci (2022) 135 (24): jcs259978. https://doi.org/10.1242/jcs.259998