rRNA sequencing : bacterial community in polystyrene microplastic biofilm under chlorination in 6 hr at 1 mg Cl/L
Biomass sample used for NGS was collected from polystyrene microplastic biofilm under chlorination in 6 hr at 1 mg Cl/L. The biomass was used for the RNA extraction. The obtained total RNA was converted to cDNA. The cDNA was used as template for high throughput sequencing. A universal bacteria primer set (i.e., 341F and 805R) was used to construct the library.