GSM8253733: RC17 cells, FGF17, 4h, rep1; Homo sapiens; RNA-Seq
Upon sample collection cells were washed once with cold PBS -/- and lysed in RLT buffer (QIAGEN). RNA was extracted using RNeasy Mini kit (QIAGEN) including on column DNase treatment (QIAGEN). Libraries were prepared using the NEBNext Poly(A) mRNA magnetic isolation module (NEB #E7490S) in combination with NEBNext Ultra II directional RNA library prep kit for Illumina (NEB #E7765) following manufacturer's protocol. Libraries were eluted in 17.5ul 0.1X TE after the final clean up.