Bulk RNA Seq of PARCB tumor derived lung cancer cell line with ASCL2 overexpression
Libraries for RNA-Seq of ASCL1/ASCL2 overexpression samples and their respective controls were prepared with KAPA Stranded mRNA-Seq Kit (Cat# KK8420, Roche). The workflow consists of mRNA enrichment and fragmentation. Sequencing was performed on NovaSeq 6000 for PE 2x150 run. Data quality check was done on Illumina SAV. Demultiplexing was performed with Illumina Bcl2fastq v2.19.1.403 software.