LPS853 Liposarcoma cell line H3K27ac hiChIP
, crosslinked chromatin was digested with MboI. Overhangs were filled in and marked with Biotin-dATP (ThermoFisher 19524016) and ends were ligated. Chromatin was then sheared on the Covaris E220 and ChIP was performed overnight for H3K27ac marked chromatin. Resulting DNA was purified using the Zymo clean and concentrate kit (DCC-100), bound to Streptavidin M280 beads (Invitrogen 11205D), and tagmented. Libraries were amplified using the Nextera DNA Library Prep kit (Illumina)