MinION sequencing; Chromatium okenii transcriptomics analysis
Chromatium okenii transcriptomics analysis
Samples for transcriptomic analysis were collectaed from the lake water column and filtered (Isopore 0.22 mm PC Membrane, diam 25 mm) using a vacuum pump connected to a filtration ramp  until the filter was completely clogged. Filters were then immediately covered with RNAlater for five minutes and stored at -20°C. RNA was extracted with the RNeasy Plus Universal Mini kit (QIAGEN) following the 'Purification of total RNA Using the RNeasy Plus Universal mini kit' protocol for the TissueLyser II For the Oxford Nanopore Technologies (ONT) library preparation, 50-100 fmol in 11 ml of reverse transcribed DNA was used and sequencing performed with an ONT R9.4 flow cell, following the manufacturer's instructions.