Illumina NovaSeq 6000 paired end sequencing; CRISPR screening of autism risk genes in brain organoids
CRISPR screening of autism risk genes in brain organoids
Cells were FACS-sorted from dissociated brain organoids at different developmental stages.  Nucleic acid were extracted using MonarchÂ® Genomic DNA Purification Kit from NEB.  Amplicon libraries were constructed by performing PCR on genomic DNA with specific primers and indexing primers.