whole exome seq of mus musculus: 2-week-old Pdx1-Cre; LSL-KrasG12D/+ male pancreas
Starting from gDNA, a shearing step produces small fragments. Prepare library with sequencer specific adaptors and indexes. Hybridize sample with biotinylated RNA library baits. Agilent uses ultra long 160mer RNA baits for the highest specificity. Select targeted regions using magnetic streptavidin beads. Amplify and load on the sequencer.