NextSeq 500 sequencing; Single cell RNA-sequencing of cells isolated from full-thickness surgically resected tissue from stricturing and non-stricturing segments of the ileum of Crohns disease patients
Single cell RNA-sequencing of cells isolated from full-thickness surgically resected tissue from stricturing and non-stricturing segments of the ileum of Crohns disease patients
ScRNA-seq of coding RNA from cells from both structuring and non-stricturing segments was undertaken. To prevent any cell type bias on RNA sequencing there was no prior selection, such as FACs sorting, involved Chromium™ 3' Library and Gel Bead Kit v3 (PN-1000092; 10X Genomics, California, USA) Chromium™ 3' Library and Gel Bead Kit v3 (PN-1000092; 10X Genomics, California, USA)