GSM6858424: A20∆BEC naive rep 3; Mus musculus; RNA-Seq
RNA was isolated from 500 sorted endothelial cells with the Qiagen RNeasy Plus Micro Kit RNA was quantified with a Qubit 2.0 fluorometer (Thermo Fisher Scientific) and RNA quality was assessed on a Bioanalyzer 2100 (Agilent) using a RNA 6000 Pico chip (Agilent). Samples with an RNA integrity number (RIN) of > 8 were used for full-length cDNA synthesis using the SMART-Seqâ v4 Ultraâ Low Input Kit (Takara). 1 ng of the resulting cDNA was used for library preparation with a Nextera XT DNA Sample Prep Kit according to the manufacturer's instructions (Illumina). Quantity was assessed using Invitrogen's Qubit HS assay kit and library size was determined using Agilent's 2100 Bioanalyzer HS DNA assay