Conidia of Colletotrichum fructicola isolate CZ102 interacted with 3- month Carya illinoinensis  (36hr post-inoculation)
Total RNA of all samples was extracted using TRIzol Reagent (Invitrogen, cat. No. 15596026). DNA digestion was carried out using DNase I. RNA quality was determined by examining A260/A280 with a NanoDrop One C spectrophotometer (Thermo Fisher Scientifific, Inc.). RNA integrity of control group was confirmed by 1.5% agarose gel electrophoresis. The RNA Integrity of three dual groups was confirmed by Qsep100 (BiOptic Inc.)/5300 Fragment Analyzer system (Agilent). Qualifified RNAs of total samples were finally quantifified by Qubit 3.0 with a Qubit RNA broad-range assay kit (Life Technologies, Q10210). 2 g total RNAs were used for stranded RNA sequencing library preparation using KC-DigitalTM Stranded mRNA Library Prep Kit for Illumina (Catalog NO. DR08502, Wuhan Seqhealth Co., Ltd. China) following the manufacturers instruction. The kit eliminates duplication bias in PCR and sequencing steps, by using UMI of 8 random bases to label the pre-amplified cDNA molecules. The library products corresponding to 200-500 bps were enriched, quantified and finally sequenced on DNBSEQ-T7 sequencer (MGI Tech Co., Ltd. China) with PE150 model.