RNA-Seq of root
The root samples were rapidly frozen in liquid nitrogen and subsequently pulverized into a fine powder. Total RNA was extracted from the samples using TRIZOL Reagent (Takara, Beijing, China), following the manufacturer's instructions. To purify mRNA, oligo(dT)-attached magnetic beads were utilized. The integrity and quality of the extracted RNA were assessed using an Agilent Bioanalyzer (Agilent, CA, USA).