GSM8426892: COG-N-453, Lorlatinib, rep1; Homo sapiens; RNA-Seq
Tumors were harvested (3 per group) and homogenized in Trizol (Invtrogen) using Quiagen TissueRuptor. RNA was extracted using Quiagen mini elute kit according to manufacturer's instructions. library preparation was done using 1mg of RNA using the Truseq total mRNA kit and ribosomal RNA was removed with Gold rRNA removal mix.