GSM8436962: Locus coeruleus from case 452; Homo sapiens; RNA-Seq
Tissue homogenization and cell lysis was performed in TRIzol. After cell lysis, impurities removal, and inhibition of RNAse activity, total RNA was extracted by using phase separation method from cell debris. Library preparation with poly A enrichment was done with the NEBNext Ultra II RNA Library Prep Kit for Illumina