GSM8031557: PGCs, F04, R3; Mus musculus; Bisulfite-Seq
PGCs (GOF18-GFP+SSEA1+) were isolated from E12.5 embryos carrying the GOF18-GFP reporter by FACS into lysis buffer (Qiagen RLT plus buffer) WGBS-sequencing libraries were prepared according to the bulk version of the published scBS-seq protocol (https://www.nature.com/articles/nprot.2016.187). Briefly, cells were lysed in RLT plus buffer (Qiagen) then bisulfite converted using the Zymo EZ-Direct kit. Converted DNA was prepared for Illumina sequencing via two rounds of random primed synthesis using oligos containing Illumina adapter sequences followed by PCR to amplify and incorporate indexes.