Metabarcoding: 16S V3V4. 10^3 CFU Salmonella-spiked caecal content
DNA extracted with DNeasy PowerSoil Pro Kit (Qiagen). V3-V4 variable regions amplified by PCR with primers 341F-785R (Klindworth 2013) and sequencing libraries were prepared according to the 16S Metagenomic Sequencing Library Preparation protocol.