Nicotiana benthamiana, PPV Infected, Replicate 3
Samples of symptomatic tissue of infected plants were collected for RNA extraction with TRIzol reagent. Contaminating DNA was removed from RNA samples with Turbo DNase (Ambion), and RNA was further purified using the RNeasy minikit (Qiagen). RNA libraries were constructed with the ScripSeq complete kit (plant leaf) (Epicenter, Illumina), including barcoding elements to identify the different samples, according to the provider's protocols. Libraries were submitted to BGI (Hong Kong) for Illumina sequencing on a HiSeq 2500 platform, and  paired-end reads were generated.