GSM8436788: VC1mM_3; Homo sapiens; Bisulfite-Seq
Genomic DNA was extracted from a half portion of human epidermal equivalents using the Monarch Genomic DNA Purification Kit (New England Biolabs, Ipswich, MA, US). The integrity and quality of the DNA were evaluated using a NanoDrop One and TapeStation (Agilent Technologies) prior to analysis. Bisulfite treatment was performed by using a Swift Biosciences Accel-NGS® Methyl-Seq DNA Library Kit (Swift Biosciences, Ann Arbor, Michigan, US) to generate WGBS libraries. The amplified libraries were sequenced using an Illumina Nova Seq 6000 with 150 bp paired-end reads following standard Illumina sequencing protocols.