GSM8330821: Ctrl, DENV1, midguts, day 9, replicate B; Aedes aegypti; RNA-Seq
Total RNA extracts from pools of 10 tissues were isolated with TRIzol (Invitrogen) and treated with DNA-free kit (Invitrogen, AM1906) following the manufacturer's instructions. The quality of the samples was assessed using a BioAnalyzer RNA Nano kit (Agilent Technologies). RNA libraries were built using an Illumina Stranded mRNA library Preparation Kit following the manufacturer's protocol depending on the insert size required. All the samples were eluted for 2 minutes at 80°C after polyA capture to obtain 300-bp inserts. Sequencing was performed on two lanes 10B300 of NovaSeqX (Illumina) by Novogen.