GSM8031815: E14; Homo sapiens; RNA-Seq
Total RNA (2-3 µg) was extracted from facial part of the embryos preserved in RNAlater and stored under -80C.  Total RNA was isolated from frozen tissues using RNeasy Fibrous Tissue Kit (Qiagen, Hilden, Germany), according to the manufacturer's protocol. The concentration and purity of extracted RNA was measured by the NanoDrop™ 8000 Spectrophotometer (ThermoFisher Scientific, Waltham, MA, USA). The RNA quality was verified by Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA ). Libraries were prepared according to the standard nAnT-iCAGE protocol (non-Amplified non-Tagging Illumina Cap Analysis of Gene Expression)