samll RNA-seq of thyroid nodule
We performed WTS on primary tumor specimens to depict the transcriptome landscape of 50 PTCs. Total RNA was isolated from tissues using Trizol Reagent (Invitrogen, Carlsbad, CA, USA), and the RNA quality was measured with an Agilent 2100 Bioanalyzer and RNA 1000 Nano LabChip Kit (Agilent, CA, USA). Then ribosomal RNA was removed using the Ribo-Zero? rRNA Removal Kit (Illumina, San Diego, USA). After removing ribosomal RNAs, the whole transcriptome library was prepared using the TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold (Illumina, San Diego, USA) and sequenced on the Illumina Novaseq 6000. A small RNA library was prepared using the TruSeq Small RNA Sample Prep Kits (Illumina, San Diego, USA) and sequenced using the Illumina Hiseq 2095