GSM6858629: HEK293 cells, shSCAF6, replicate 2; Homo sapiens; RNA-Seq
Total RNA was isolated using DirectZol RNA Miniprep kit (Zymo Research). 1.3-1.7 ug RNA per sample was used for library construction. Libraries were prepared at the University of Texas Genomic Sequencing and Analysis Facility (GSAF) according to manufacturer's instructions for the NEBNext Ultra II Direction RNA kit (NEB). Strandness of library was preserved using dUTP method.