GSM7036256: ATAC_Activated_2D2_WT_Th17_rep3; Mus musculus; ATAC-seq
1 X 10^4 cells were pelleted and washed with 50 μl 1× PBS. After pelleting the nuclei by centrifuging at 500 × g for 10 min, the pellets were re-suspended in 50 μl transposase mixture (25 µl of 2x TD buffer, 2.5 µl of TDE1, 0.5 µl of 1% digitonin, 22 µl of nuclease-free water) (#FC-121-1030, Illumina; #G9441, Promega). The reaction was incubated at 37°C with shaking at 300 rpm for 30 min. The fragmentalized DNAs were then purified using a QIAGEN MinElute kit and amplified with 10 or 11 cycles of PCR based on the amplification curve, and the paired-end libraries were purified using a QIAGEN PCR cleanup kit Indexed libraries were multiplexed and sequenced for 50 paired-end read cycles on NextSeq 550 instrument according to the manufacturer's instructions