16S rRNA amplicon sequencing of municipal landfill methane capture system biofilm BF4
16S rRNA amplicon sequencing on a MiSeq (Illumina) was conducted by MetagenomBio (https://metagenom.co/). The V4 16S rRNA region was amplified using forward primer 515FB (5- GTGYCAGCMGCCGCGGTAA-3) and reverse primer 806RB (5- GGACTACNVGGGTWTCTAAT-3). Amplified DNA was sequenced on the MiSeq platform with library preparation using the MiSeq Reagent Kit v2 (2 x 250 cycles).