GSM8032483: Igh-Igk-hybrid-D_v-Abl_Jk5_rep1; Mus musculus; OTHER
DNA was lysed overnight at 56degC in a lysis buffer containing: 200mM NaCl, 0.2% SDS, 10mM Tris-HCl pH7.4, 2mM EDTA pH8.0, 200ug/ml proteinase K, precipitated by isopropanol and dissolved in buffer containing 10mM Tris-HCl pH7.4, 0.5mM EDTA pH8.0 HTGTS-V(D)J-seq libraries were prepared using linear-amplification PCR-mediated high-throughput genome-wide translocation sequencing protocol with indicated bait primers