AP4 ChIP-Sequencing of PARCB tumor derived prostate cancer cell line
The CUT&RUN experiment was performed using previously established method (Skene et al., 2018) (Skene et al., 2018) and the manufacturer's protocol (Cat# 86652, Cell Signaling). 100k live cells were used per reaction. 50pg of Spike-In DNA (Cat# 12931, Cell Signaling) was added per reaction for downstream normalization. DNA was purified using MinElute PCR Purification Kit (Cat# 28004, Qiagen), followed by fragmentation by using sonicator (Cat# M202, Covaris). Dual size selection was applied using KAPA Pure beads (Cat# KR1245, Roche). DNA Libraries were prepared with the KAPA DNA HyperPrep kit (Cat# KK8504, Roche).