GSM7886284: RPE1_CTRL_KO_2; Homo sapiens; RNA-Seq
Cells were washed twice with PBS and lysed in 1 ml TRIzol on ice. Lysates were frozen in liquid nitrogen and stored at -80℃. RNA library preparation and sequencing was performed at Novogene (Beijing). Briefly, sequencing libraries were generated from total RNA using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, Cat.No. E7530L) following manufacturer's recommendations. After random fragmentation, 370~420 bp cDNA fragments were selected for paired end sequencing of 150bp.