GSM6533111: SupT1, Uninfected, rep2; Homo sapiens; RNA-Seq
Total RNA was extracted from flow-sorted cells using TRIzol reagent (Invitrogen) according to manufacturer's protocol, followed by treatment with DNase (Turbo DNAse-free kit, Invitrogen)Ribosomal RNA (rRNA) was removed from the total RNA (50ng) using NEBNext rRNA Depletion kit v2 (Human/Mouse/Rat, New England Biolab), according to manufacturer's instructions. The rRNA-depleted samples were used for stranded library preparation using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs).