GSM7339688: homozygous male mouse, Control, m6A IP, biol rep 4; Mus musculus; RIP-Seq
Total RNA was then extracted from iBAT by TRIzol. 1 μg of mRNAs was isolated from total RNA using a Dynabeads mRNA DIRECT purification kit (Thermo Fisher). Then, mRNA was adjusted to about 10 ng μl−1 in 100 μl and fragmented using a Bioruptor ultrasonicator (Diagenode) with 30 s on/off for 30 cycles. m6A immunoprecipitation (m6A-IP) and library preparation were performed using the EpiMark N6-Methyladenosine enrichment kit (NEB). RNA libraries for RNA-seq were prepared using TruSeq RNA Library Prep Kit following manufacturer's protocols.