WES of a CTLA4 patient
Genomic DNA was extracted from the peripheral blood of the proband (Tiangen, China) and fragmented to 180-280 bp using Covaris. Then, fragments were ligated with adapters based on NEBNext UltraII DNA Library Prep Kit for Illumina (NEB, USA). Exome capture and library construction were prepared using Agilent SureSelect Human All Exon V6 Capture Kit (Agilent Technologies, Santa Clara, CA, USA), followed by exome sequencing to an average coverage of 100 on Illumina NovaSeq 6000 System with 2  150 bp paired-end reads.