GSM7994749: circRNA long reads sequencing; Homo sapiens; ncRNA-Seq
Cells were harvested at 24 and 48 h post-infection with TRIzol RNA Isolation Reagents (Invitrogen, USA, Catalog number: 15596026). Chloroform extraction and isopropanol precipitation methods were used to isolate RNA from samples. Ribosome-depleted and RNase R-treated RNA samples were used for library preparation and nanopore long-read sequencing, which combines rolling circular reverse transcription and nanopore sequencing.