GSM6481355: IP, ETV6, A; Homo sapiens; ChIP-Seq
Chromatin immunoprecipitation was performed from 80 millions monocytes as starting material, using the ChIP-IT High Sensitivity kit (Active Motif) following manufacturer's instructions. Monocytes were homogenized using a 7 mL Dounce glass homogenizer (Clinisciences). Sonication was performed using a Bioruptor Pico instrument (Diagenode) using 20 cycles (30 seconds on and 30 seconds off). Chromatin was immunoprecipitated using antibodies against ETV3 (ref A303-737A, polyclonal, Bethyl) or ETV6 (clone R1092.1.1A9, CDI Laboratories Inc). Libraries were prepared according to Illumina's instructions accompanying the TruSeq ChIPSeq Library Prep Kit (Illumina). 4-6 ng of DNA was used for each sample. Sequencing was performed in 1 sequencing unit of NovaSeq 6000 (Illumina) (100-nt593 length reads, paired end).