GSM7886056: K36M, 2wk,rep2; Mus musculus; RNA-Seq
Organoids were harvested at each timepoint using EDTA to remove matrigel and TrypLE to digest to single cell, at which point RNA was isolated using Zymo Direct-zol RNA Miniprep kit. Libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina. Post amplification libraries were purified using Agencourt AMPure XP beads from Beckman Coulter. Libraries were validated using Agilent High Sensitivity DNA kit.