DNA sequence of ASFV from affected pigs
The paired-end sequencing libraries were prepared from the QC passed viral DNA samples usingillumina TruSeq Nano DNA Library Prep Kit. Briefly, approximately 200ng of DNA wasfragmented by Covaris M220 to generate a mean fragment distribution of 350bp. Covarisshearing generates dsDNA fragments with 3' or 5' overhangs. The fragments were then subjectedto end-repair. This process converts the overhangs resulting from fragmentation into blunt endsusing End Repair Mix. The 3' to 5' exonuclease activity of this mix removes the 3' overhangs andthe 5' to 3' polymerase activity fills in the 5' overhangs followed by adapter ligation to thefragments.