GSM7232829: F38, spleen, Triploid, Day3; Oncorhynchus tshawytscha; miRNA-Seq
Total RNA was extracted from each tissue using the mirVana miRNA isolation kit (Ambion, AM1560/1561). Flash-frozen tissues were ground over liquid nitrogen before following manufacturer's protocol without deviation. The NEBNext Small RNA Library Prep (New England Biolabs, E7330) was used to generate libraries for each sample with RNA inputs depending on sample concentration (300 ng for hindgut and 100 ng for spleen/head kidney).