RNA-seq of Corynebacterium glutamicum wild-type
To compare the wild-type and mprAB mutant strains, the single clone was grown in LBB medium at 30 C for 16 h. The pre-culture was transferred into CGX medium containing 4% glucose for an initial OD600=0.5 and then harvested at OD600=5.0-6.0. Cells were washed three times using PBS buffer (137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO4, 1.4 mM KH2PO4), harvested by centrifugation at 4C, and the pelleted cells were quick-frozen in liquid nitrogen. RNA-Seq was performed by Novogene (Beijing, China). The comparisons were performed using three biological replicates.