Metagenomic sequencing of environmental community
We used the Native Barcoding Kit (SQK-NBD114.24) for sequencing library preparation to multiplex 10 cores from this site. We sequenced the library using a MinION (MinKNOW Core version 5.4.7) on a R10.4.1 Flow cell (FLO-MIN114) for 72 hours. We base called the raw sequencing data with Guppy version 6.5.7 using the super-accuracy model (dna_r10.4.1_e8.2_400bps_sup.cfg) and filtered by minimum quality score (Q score  10).