GSM6755056: 280520_PY01_liver; Trichosurus vulpecula; ssRNA-seq
400 ng of DNase-treated RNA was used to construct poly(A) enriched, strand-specific RNA sequencing libraries using the NEXTFLEX Rapid Directional RNA-Seq Kit 2.0 and NEXTFLEX Poly(A) Beads (2.0) (Perkin Elmer) Total RNA was extracted from tissue samples (~50 mg) stored in RNAlater using TRIzol Reagent (Invitrogen) following the manufacturer's guidelines. RNA samples were treated to remove any contaminating DNA using the Turbo DNA-free kit (Invitrogen) according to the protocol.