Sc-Seq of mus musculus: stomach
Single cell suspension of stomach was isolated from mice w/ or w/o H.felis infection and then flow-sorted to collect CD45+ immune cells. There were 2 uninfected libraries and 3 H.felis infected libraries, 6 mice in each library. Each cell suspension was subjected to 3' single-cell sequencing using 10x Chromium Controller with accompanying Chromium Next GEM 3' Kit V.3.. Libraries were sequenced on Illumina NovaSeq 6000 platform.