GSM7633484: scRNA-seq PMBC, healthy donor 6; Homo sapiens; RNA-Seq
Viably frozen PBMC were thawed, washed with PBS to remove dead cell debris and resuspended to 1,000 cells/µl. The cells were loaded to the Chromium controller for GEM generation 10x Genomics' protocol. We targeted 10,000 sequenced cells. We only selected representative patients from each treatment group whose PBMC cell quality and quantity were sufficient for single cell RNAseq processing. Sequencing library was constructed per manufacturer's protocol (10x Genomics, 3' scRNAseq)