GSM8332461: PV-3 hour0; Pseudomonas veronii; RNA-Seq
RNA is extracted from cells using standard extraction methods, followed by an Agilent 2100 bioanalyzer for precise measurement of RNA integrity and total RNA. Firstly, ribosomal RNA was removed from total RNA, followed by ethanol precipitation. After fragmentation, the first strand cDNA was synthesized using random hexamer primers. During the second strand cDNA synthesis, dUTPs were replaced with dTTPs in the reaction buffer. The directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification.