GSM8120830: CR, FOXO1_ChIP, rep2; Mus musculus; ChIP-Seq
Snap frozen livers were lysed and fixed with 4% formaldehyde for 15 minutes at room temperature. After quenching and cell lysis, chromatin was sonicated using Diagenode Bioruptor. Libraries from ChIP DNA were prepared with the KAPA Hyperprep Kit (Kapa Biosystems), Illumina compatible adapters were synthesized by IDT (Integrated DNA Technologies). Adapter-ligated libraries were size selected (360-610bp) in a Pippin Gel station (Sage Science) using 2% dye free gels (Sage Science). Library concentrations were estimated by RT-PCR with the KAPA Library Quantification Kit (Kapa Biosystems). Quality of the libraries was evaluated with the Agilent High Sensitivity DNA Kit in a 2100 Bioanalyzer (Agilent).