GSM7622119: h3k4me3, ll, 26ºC, rep3; Ostreococcus tauri; ChIP-Seq
Chromatin was fixed with 1% Formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated with 15 cycles of 30 seconds ON 30 and seconds OFF using a Bioruptor sonicator (Diagenode). Anti-H3K27me3 (Diagenode, C15410069) and anti-H3K4me3 (Diagenode, C15410003) antibodies were used for chromatin immunoprecipitation. The libraries were prepared with Takara's ThruPLEX DNA-Seq kit. The quality controls of the generated libraries were evaluated by the Bioanalyzer DNA High sensitive chip for which the samples were previously measured by fluorimetry (Qubit®).