Illumina NovaSeq 6000 paired end sequencing; Human embryonic yolk sac CITE-seq data
Samples were collected by the Human Developmental Biology Resource from the clinic and transported to the lab immediately before dissection. Yolk sacs were digested for 30 mins using collagenase type 4 to create a single cell suspension.  Cells were FACS sorted based on their protein expression of CD34. Contiguously placed CD34 positive and CD34 negative gates were used so to sample the full spectrum of expression. Cells were sorted into FACS tubes containing flow buffer coated with FCS then subsequently used for CITE-seq. Nucleic acids were extracted following the manufacturers guidelines for the 10x Genomics Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry) (CG000204_ChromiumNextGEMSingleCell3'v3.1_Rev D.pdf). CITE-Seq libraries were amplified following the TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3' Reagent Kit v3 or v3.1 (Single Index) Protocol (https://www.biolegend.com/en-us/protocols/totalseq-a-antibodies-and-cell-hashing-with-10x-single-cell-3-reagent-kit-v3-3-1-protocol). Nucleic acid libraries were constructed following the manufacturers guidelines for the 10x Genomics Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry) (CG000204_ChromiumNextGEMSingleCell3'v3.1_Rev D.pdf). CITE-Seq libraries were constructed following the TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3' Reagent Kit v3 or v3.1 (Single Index) Protocol (https://www.biolegend.com/en-us/protocols/totalseq-a-antibodies-and-cell-hashing-with-10x-single-cell-3-reagent-kit-v3-3-1-protocol).