GSM6718763: Cre+ pMBMEC, untreated, rep1; Mus musculus; RNA-Seq
Total RNA was extracted using the RNeasy Mini Kit (Qiagen). RNAseq libraries were prepared from 500 ng of total RNA. Illumina TruSeq Stranded mRNA reagents (Illumina) using a unique dual indexing strategy was used, and following the official protocol automated on a Sciclone liquid handling robot (PerkinElmer).