GSM7793997: sfGFP-1 IN; Pseudomonas aeruginosa; RNA-Seq
RNA isolation was performed with phenol/chloroform extraction and purified on spin columns (Zymo Research). cDNAs were treated with ExoSAP-IT (Affymetrix) to remove the excess oligonucleotides. Second DNA adapters (containing 5 [N5] or 10 [N10] random bases at the 5′-end) were ligated to the 5′-end of the cDNA (T4 RNA Ligase, NEB). The DNA was amplified by PCR and purified with PippinPrep system (Sage Science) and sequenced with Illumina HiSeq 4000.