GSM6859116: C140, hippocampus, MiSeq; Homo sapiens; RNA-Seq
Hippocampal and frontal cortical samples were cryosectioned at -20°C. The 10 μm sections were placed on a Visium Tissue Optimization Slide (10x Genomics, #3000394) and Visium Spatial Gene Expression Slide (10x Genomics, #2000233). By following the Visium Spatial Tissue Optimization User Guide (10x Genomics, #CG000238 Rev E), the optimal permeabilization time was determined to be twelve minutes. The gene expression slides underwent methanol fixation, Hematoxylin & Eosin (H&E) staining, and brightfield imaging using a 10x objective on a Nikon Eclipse Ti2-E (10x Genomics, #CG000160 Rev C). Immediately after imaging, the tissues were permeabilized and the remaining steps for library construction were completed according to the user guide (10x Genomics, #CG000239 Rev F).