GSM7440359: THP_WT_VPX_2; Homo sapiens; RNA-Seq
Cells were incubated in 37°C, 5 % CO2 for 16 hours after transfection with 5 µg of pcDNA3.1-Vpx-NeGFP, pcDNA3.1-MutVpx-NeGFP or pcDNA3.1-NeGFP plasmids, then washed in 1 ml ice-cold PBS twice. Total RNA was extracted using TRIzol Reagent (Thermo Fisher Scientific, MA, USA) according to the manufacturer's instructions. Experiments were carried out as duplicates. RNA-Seq libraries were prepared from total RNA using Ultra II RNA Sample Prep kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturer's protocol.