GSM7623740: M2; Mus musculus; RNA-Seq
Total RNA was extracted from the tissue using TRIzol® Reagent  according the manufacturer's instructions (Invitrogen) and genomic DNA was removed using DNase I (TaKara).Then RNA quality was determined by 2100 Bioanalyser (Agilent) and quantified using the ND-2000 (NanoDrop Technologies). Only high-quality RNA sample(OD260/280=1.8~2.2, OD260/230≥2.0, RIN≥6.5, 28S:18S≥1.0, >1μg) was used to construct sequencing library. The high-throughput sequencing and analyses for mRNAs were carried out by Wefind Biotechnology Co., Ltd. (Wuhan, China). After the samples were qualified, the library was constructed according to the Illumina sample preparation process of the RNA-seq protocol. The purified double-stranded cDNA was selected for fragment size using AMPure XP Beads. The DESeq2 software was used to analyze the differential expression between the sample groups.