GSM7039296: N3; Mus musculus; RNA-Seq
Total RNA from sorted VAT ILC2s of wild-type mice fed with normal-diet and high-fat-diet was extracted by Trizol reagent.  RNA quality was examined with an Agilent Bioanalyzer 2100 (Agilent). The SMART-Seq HT Kit (Takara Bio USA, Inc) was used to generate high-quality, full-length cDNA. Libraries were generated using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme) following manufacturer's protocol.