GSM6718722: EA9R_Medulla; Homo sapiens; RNA-Seq
Total RNA was extracted using Agencourt RNAdvance Tissue kit including DNase treatment for 15 min at 37 ̊C (Ambion DNaseI, ThermoFisher) on an automated Biomek i7 Hybrid system (Beckman Coulter). KAPA RNA HyperPrep Kit with RiboErase (Roche) with 450ng of input RNA, 5min fragmentation at 95  ̊C, and 9 PCR cycles performed on a Tecan Fluent 1080 system (Tecan).