GSM6756062: 92.1_IgG_CST; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using an ultrasonicator (Thermo Fisher). ChIP-seq libraries were constructed by Novogene Corporation (Beijing, China). DNA purity was checked using the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA). DNA concentration was measured using Qubit® DNA Assay Kit in Qubit® 3.0Flurometer(LifeTechnologies,CA,USA). Pair-end sequencing of sample was performed on Illumina platform (Illumina, CA, USA). Library quality was assessed on the Agilent Bioanalyzer 2100 system.