GSM8257360: LNCaP-sgPRKCI_ENZA_H3K27me3; Homo sapiens; OTHER
The cells were incubated overnight with conA beads and specific antibodies including K-metstat internal control at 4C. Chromatin were digested using pAG-MNase for 15 minutes at RT. The fragmented DNA was column purified. CUT&RUN libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for illumina. 5ng of CUT&RUN DNA were used to prepare the libraries using specific index primers and universal PCR primer and amplified for 14 cycles in a thermocycler. Post amplification, the libraries were size selected for 300-400bp using SPRIselect beads from NEB. The concentration and size of the libraries were measured using Qubit and Agilent High Sensitivity DNA kit respectively.