GSM7698008: Tc17-CD5-lo; Mus musculus; RNA-Seq
RNA was harvested using NucleoZOL (Macherey-Nagel). 1.5 ug of total RNA was used for the construction of sequencing libraries. Total RNA was extracted using TRIzol® RNA Isolation Reagents (Life technologies). The quantity and quality of the total RNA were evaluated using Agilent 2100 bioanalyzerRNA kit (Agilent). The isolated total RNA was processed for preparing mRNA sequencing library using the Illumina TruSeqStranded mRNA Sample Preparation kit (Illumina) according to the manufacturer's protocol. Quality and size of libraries were assessed using Agilent 2100 bioanalyzerDNA kit (Agilent). All libraries were quantified by qPCR using CFX96 Real Time System (Bio-Rad) and sequenced on the NextSeq500 sequencers (Illumina) with a paired-end 75bp plus single 8bp index read run.