GSM6964698: H3K27ac_DNMT_KO_EpiLC_D2_rep2; Mus musculus; ChIP-Seq
Chromatin from 10 million cells was sonicated to between 200-600 bp using a Bioruptor Plus (Diagenode). 100µL of Dynabeads Protein G (Invitrogen, 10003D) were used per immunoprecipitation. The following antibodies were used: H3K4me3 (Abcam, ab8580), H3K27me3 (Millipore, 07-449), H3K4me1 (Active Motif, 39297), H3K27ac (Abcam, ab4729), CTCF (Millipore, 07-729). Libraries were prepared using the NEBNext Ultra II Library Prep Kit for Illumina (New England Biolabs, E7645) following the manufacturer's instructions. Libraries were size selected after amplification using Agencourt Ampure XP beads (Fisher Scientific, 10136224) prior to pooling and sequencing.