GSM6922903: 2x BNT162b2 + BNT162b2:BNT162b4, B cells, CITE [G12-B-CITE]; Mus musculus; OTHER
Splenocytes from immunized animals were thawed and incubated with fluorescently-labelled S protein or S pMHC tetramers. Antigen-positive cells were sorted using the BD FACSAria Cell Sorter, and up to 8000 cells per animal were collected. Cells were pooled by treatment group, and each group was loaded into a channel on the 10X Chromium Connect using Chromium Next Automated GEM 5' v2 kit. Gene expression libraries were generated using the automated library construction kit, CITEseq libraries were generated using the Chromium Automated 5' Feature Barcode kit and VDJ libraries were generated using Chromium Automated Mouse BCR/TCR Amplification and Library Construction kit. Sequencing was carried out on the Illumina NovaSeq 6000 platform with an S1 flow cell.