GSM6481635: oDam-Fer1 replicate 2; Drosophila melanogaster; OTHER
50-60 midguts were dissected for each sample, and total genome DNA was extracted, followed by DpnII digestion, dephosphorylation (twice the amount of enzymes and treatment time compared to the original protocol), DpnI digestion and T4 ligation of adaptors. Afterwards, fragments were amplified by ligation-mediated PCR, T7 exonuclease treatment and purification, yielding DNA products ranging from 200 to 2000bp. Finally, the products were fragmented and carrying out subsequent library preparation and high-throughput sequencing. The NEB Next Ultra II DNA library prep kits (New England Biolabs, cat. no. E7645L) was used for library preparation following the protocal provided by the manufacturer