GSM8292076: K562, delEX1, H3K27ac, rep2; Homo sapiens; ChIP-Seq
Cells were crosslinked with 1% formaldehyde for 10 minutes. Chromatin was sheared by sonication. Immunoprecipitation was performed overnight with the corresponding primary antibody. DNA was purified from A/G beads using phenol-chloroform. ChIP-seq libraries were prepared using NEBNext Ultra Library Prep Kit for Illumina according to the manufacturer's conditions.