GSM8143853: SCN, control1, 2hours, rep4; Mus musculus; RNA-Seq
Total RNA from each sample replicate was extracted using RNeasy Micro Kit (QIAGEN, #74004) following the protocol for microdissected cryosections as per manufacturer's guidelines and stored at -80°C. PolyA RNA-Seq libraries were prepared by Oxford Genomics Centre, University of Oxford using NEBNext Ultra II Directional RNA Library Prep Kit (NEB, #E7760) and sequenced on NovaSeq6000_150PE (150 bp paired-end directional reads: ~40 million reads/sample) platform.