GSM7350006: Lin28bTg, 5 Days activated, 2; Mus musculus; RNA-Seq
20x10^6 in vitro-generated CTLs and at least 4x10^6 naive and 12h-activated CD8+ T cells were lysed in TRIzol (Invitrogen) and sent to Novogene for RNA extraction, library preparation, sequencing and initial data processing. RNA quality was assessed by Nanodrop, Agarose gel electrophoresis, and by the Agilent 2100 Bioanalyzer. RNA-Seq libraries were prepared using the HiSeq X Ten Reagent Kit following manufacturer's protocols.