GSM7623321: ATAC-Seq_INO80_degron_0h_rep2; Mus musculus; ATAC-seq
Extracted DNA according to the DNA extraction kit's instruction After the INO80, P400 and SRCAP degradation, ATAC-seq were performed as depicted before and following the TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme, TD501). 50000 viable cells were collected for penetrated with 0.1% NP40, 0.1% Tween-20, and 0.01% Digitonin in 50 μl cold RSB buffer, then fragmentated and tagged using the Tn5 transposase according to the kit manufacture for 30 min at 37°C in a thermomixer with 1000 rpm mixing. Reaction was stopped by addition 250 μl (5 volumes) of DNA binding buffer, and extracted DNA according to the DNA extraction kit's instruction. The eluted DNA were amplified 8 cycles for library generation using the Illumina primers, and 200-500 bp fragments were selected for sequencing analysis.