GSM8332495: Primary astrocyte transfected with control miRNA mimic (cel-miR-67)_sample2; Mus musculus; RNA-Seq
Extraction of RNA from cultured cells was performed using the miRNeasy Mini Kit (217004, Qiagen, Venlo, Netherlands) according to the manufacturer's instructions. RNA-Seq libraryies for RNA-Seq were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with NEBNext Poly(A) mRNA Magnetic Isolation Module following manufacture's protocols.