GSM8292079: K562, WT, Input, rep1; Homo sapiens; OTHER
Cells were crosslinked with 1% Glutaraldehyde for 10 minutes. Chromatin was sheared by sonication. Immunoprecipitation was performed overnight with the corresponding probes. DNA was purified from A/G beads using phenol-chloroform. ChIRP-seq libraries were prepared using NEBNext Ultra Library Prep Kit for Illumina according to the manufacturer's conditions.