GSM7734668: Subject_1_Tissue; Homo sapiens; RNA-Seq
Leukocytes were collected from whole blood using Histopaque and periprosthetic tissue was dissociated using a mortar and pestle in ice-cold HBSS + 10% FBS to obtain a single cell suspension. The resulting cell suspensions were passed through a 70um filter to remove aggregates and processed for FACS.