Illumina NovaSeq 6000 paired end sequencing; RNA-seq of  LSK isolated from Zrsr2 mutant, Tet2 mutant and Zrsr2/Tet2 double mutant against wild-type controls
Bone marrow LSK cells were sorted from a pool of either WT, Zrsr2mut/mut, Tet2-/- and Zrsr2mut/mut Tet2-/- mice. Total RNA was extracted from LSK cells using the RNeasy Micro kit (Qiagen) following the manufacturer's instructions. On-column DNAse treatment was performed. Low input Directional RNA-seq library was prepared using NEBNext® Ultra Directional RNA Library Prep Kit for Illumina® (Cat No. 7420).