GSM8072183: HA_OE_DBDmut_Donor_5; Homo sapiens; RNA-Seq
Total RNA was isolated with Trizol, followed by precipitation with isopropanol. RNA was washed with 75% Ethanol and pellet was dried and dissolved in nulcease-free water. Approximately 20ng total RNA was used for library preparation with Ovation Ultralow RNA-seq V2 (Tecan) from two biological replicates. Libraries were generated according to the manufacturer's instructions. Approximately 50ng amplified cDNA was subjected to Ovation Ultralow V2 library generation and manufacturer's instructions were followed. Libraries were size selected with E-Gel EX 2% agarose gels (Life Technologies) and purified by QIAquick Gel Extraction Kit (Qiagen). Libraries were sequenced on HiSeq 6000 instrument.