DNBSEQ-G400 paired end sequencing; RNA-seq of human osteoclasts treated with anti-MDA or control antibodies
Collection of adherent cells from osteoclast cultures Peripheral blood monocytes (5X10^5/ml) cultured in DMEM+10% FBS in the presence of 25ng/ml M-CSF for 3 days. 50% of the medium was replaced by then and 15ng/ml M-CSF and 1ng/ml RANK-L were added. The cells were cultured for 3 more days. Human monoclonal antibodies 146+:01G07 or 1362:01E02 were added to the cultures in the last 3 days in 5microgram/ml concentration or the cells were left without antibody treatment. RNEeasy PLUS from Qiagen Oligo-dT