GSM8208720: HCT116_invMDM2_low; Homo sapiens; OTHER
Cells were lysed in a buffer containing 0.1M NaCl, 0.038M TrisHCl, 0.01M EDTA and 0.175M SDS. DNA was extracted using the phenol-chloroform method  followed by ethanol precipitation. After PicoGreen quantification, 42-100 ng of genomic DNA were used for library construction using the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) with 8 cycles of PCR. Barcoded libraries were sequenced on the NovaSeq 6000, using the NovaSeq 6000 S2 or S4 Reagent Kit (200 or 300 Cycles) (Illumina) for PE100 reads. An average of 26M reads were generated per sample, corresponding to 0.7X coverage of the genome.