GSM6755445: PRC2 WT iMEFs PRC2i ctrl rep1 [D448-D447T100 RNA-seq]; Mus musculus; RNA-Seq
To isolate RNA from MEFs, cells of all experimental conditions were grown simultaneously and RNA was extracted 3 days after plating 200,000 cells. Total RNA was isolated using Trizol-Chloroform extraction and isopropanol precipitation. cDNA was further obtained using the High Capacity cDNA RT kit.  RNA from FL samples was extracted using the RNeasy mini kit (Qiagen) according to manufacturer's instructions. RNA quality was assessed on an automated Tapestation 4200 platform. RNA Integrity Numbers (RINs) were used to evaluate the integrity of RNA samples with > 7.0 considered intact and < 7.0 considered degraded. iMEFs RNA-Seq libraries were prepared using the Swift Biosciences RNA library kit. FL RNA-Seq libraries were prepared using the Illumina TruSeq Stranded mRNA kit.