GSM7962162: HEK293T, tRNA-R-TCT-4 overexpressed, rep 1; Homo sapiens; ncRNA-Seq
total RNA was extracted from cells using TRIzol reagent (Life Technologies) RNA libraries were prepared using modified eCLIP library construction, NEBNext Small RNA library kits (NEB) and SMARTer Stranded Total RNA-Seq kits (v3) - Pico Input mammalian (Takara) Library Prep Kit following manufacturer's protocols.