GSM7441182: WT_Small_Intestine_Macrophages; Mus musculus; RNA-Seq
Individual intestines were stripped of mucus and epithelial cells and processed into single cell suspensions using Liberase TL enzymatic digestion and mechanical disruption, filtered through 70 and 40um strainers. 3-4 intestines were combined and stained for FACS to isolate Live CD45+Lin-(CD3,TCRb,B220,SiglecF,Ly6C,Ly6G)CD11b+MHCII+CD64+ Macrophages. Sorted cells were loaded onto the 10X Genomics platform and subsequent sequencing. Library construction was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics).