GSM7634673: S1_HRG, 1 hr, ATAC-Seq, rep 2; Homo sapiens; ATAC-seq
Briefly, 50,000 single cells were lysed using cell lysis buffer (0.1% NP-40, 0.1% Tween-20, and 0.01% Digitonin in resuspension buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, and 3 mM MgCl2 in water) for 3 min on ice.  After washing using resuspension buffer containing 0.1% Tween-20, cells were centrifuged at 500 g for 10 min at 4°C. The transposition reaction was performed using Tagment DNA enzyme and buffer (Illumina, catalog no. 20034197) at 37°C for 30 min in an Eppendorf ThermoMixer (1,000 rpm). DNA was purified using the MinElute Reaction Cleanup Kit (Qiagen, catalog no. 28204). Libraries were prepared by amplifying DNA for 5-9 cycles using DNA/RNA UD Indexes Set A (Illumina, catalog no. 20027213) and NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs, catalog no. M0541S). AMPure XP bead purification (Beckman Coulter, catalog no. A63880) was used for the PCR product cleanup. Libraries were prepared by amplifying DNA for 5-9 cycles using DNA/RNA UD Indexes Set A (Illumina, catalog no. 20027213) and NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs, catalog no. M0541S). AMPure XP bead purification (Beckman Coulter, catalog no. A63880) was used for the PCR product cleanup. The libraries were validated with Agilent Bioanalyzer DNA High Sensitivity Kit and quantified with qPCR at the IGC