GSM6482920: cHiC_ limb_E115_deltaD1_mouse_Rep-1; Mus musculus; Hi-C
A S-Series 220 Covaris was used to shear the DNA to fragments of 300–600 bp for library preparation, and biotin-filled DNA fragments were pulled down using Dynabeads MyOne Streptavidin T1 beads. DNA ends were subsequently repaired using T4 DNA polymerase and the Klenow fragment of DNA polymerase I and phosphorylated with T4 Polynucleotide Kinase NK. DNA was further prepared for sequencing by ligating adaptors to DNA fragments, using the NEBNext Multiplex Oligos for Illumina kit. Indexes were added via PCR amplification (4–8 cycles) using the NEBNext Ultra II Q5 Master Mix. PCR purification and size selection were carried out using Agencourt AMPure XP beads. Libraries were sequenced on a x platform yielding x bp paired-end reads. For each sample, the HiC library was created by pooling a total of four technical replicates generated from two different cell isolations cultures in order to ensure higher complexity of the sequencing library