GSM7519338: 2524-SY-1; Homo sapiens; RNA-Seq
Whole blood was collected on the day of enrollment and buffy coats transferred to a separate 50 mL conical. For red blood cell lysis, 20 mL of 0.2% NaCl was added. After a 45-60 second incubation, 30 mL of 1.8% NaCl was added. Cells were washed in PBS and then resuspended in PBS at a concentration of 10^6 cells/mL for library preparation. All centrifugations were performed at room temperature. scRNA-seq libraries were prepared by the University of Michigan Advanced Genomics Core using the Chromium single cell 3′ library and gel bead kit v3 (10x Genomics) according to the manufacturer's protocol.