GSM7918309: Ly6GC negative cells, CSP; Mus musculus; OTHER
Ly6G+Ly6C+ and Ly6C+G- cells (13,627 and 1,624, respectively) were processed using a 10X Genomics platform by the UNMC Genomics Core. Briefly, single cells were lysed, RNA was reverse-transcribed and barcoded using a Chromium Single-Cell 3' Reagent Kit v3 (10X Genomics) according to the manufacturer's protocol. Illumina compatible cDNA libraries were created and quantified by quantitative PCR using the KAPA Library Quant Kit (Illumina) and were loaded at a concentration of 1.3 pM on an Illumina NovaSeq instrument. Samples were sequenced following the parameters suggested by 10X Genomics to an average read depth of 100,000 reads/cell.