GSM7697891: EmptyTim3-2; Mus musculus; ATAC-seq
Transposed DNA fragments were purified using a Qiagen Reaction MiniElute Kit, barcoded with NEXTERA dual indexes (Illumina) and amplified by PCR for 11 cycles using NEBNext High Fidelity 2x PCR Master Mix (New England Biolabs) PCR products were purified using a PCR Purification Kit (Qiagen) and amplified fragments size was verified on a 2200 TapeStation (Agilent Technologies) using High Sensitivity D1000 ScreenTapes (Agilent Technologies). Libraries were quantified by qPCR using a KAPA Library Quant Kit (KAPA Biosystems). Normalized libraries were pooled, diluted to 1.8pM, loaded onto a NextSeq 500/550 High Output Kit v2.5 (150 cycles, Illumina) and paired-end sequencing was performed on a NextSeq 550 (Illumina)