GSM8330097: HL60-Suc-0h-R3; Homo sapiens; RNA-Seq
Total RNA was extracted using RNAiso Plus Total RNA extraction reagent (Cat#9109, TAKARA)following the manufacturer's instructions and checked for a RIN number to inspect RNA integrity by an Agilent 2100 Bioanalyzer (Agilent technologies, Santa Clara, CA, US). According to the experimental protocol, purified Total RNA was used for mRNA isolation, fragmentation, first strand cDNA synthesis, second strand cDNA synthesis, end repair, 3' end A addition, adapter ligation, and enrichment to construct the sequencing library. RNA Libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacture's protocols