NextSeq 500 sequencing; Culture associated DNA methylation changes impact on cellular function of Human Intestinal Organoids (Expression)
Human IEO were generated from mucosal biopsies by isolation of intestinal crypts and culturing in MatrigelÂ® (Corning) At the end of each experiment, human IEO were harvested and both DNA and RNA were extracted using the AllPrep DNA/RNA mini kit (Qiagen, Hilden, Germany).  RNA was prepared with the Truseq mRNA library preparation (Illumina, San Diego, CA, USA)