GSM7697999: HL60_DAC_1; Homo sapiens; RNA-Seq
Total RNA was extracted using an RNeasy Mini Kit (Qiagen), and RNA integrity and purity were checked using Agilent 2100 Bioanalyzer (Agilent Technologies). 1.0μg of total RNA was used for the construction of sequencing libraries. After mRNA enrichment using oligo(dT) beads and rRNA depletion using the Ribo-Zero Plus rRNA Depletion Kit (Illumina), cDNA was synthesized using an mRNA template and random primers, second-strand synthesis buffer, dNTPs, RNase H and DNA polymerase I. The double-stranded cDNA library was completed by “A” base ligation and sequencing adaptor ligation. The quality and quantity of these libraries were checked using Qubit 2.0 (Thermo Fisher Scientific), Agilent 2100, and quantitative PCR using the Real-time PCR Systems Step One Plus (Applied Biosystems).