GSM8120473: 43728 Lung cancer tumor nest CD68+ ROI 5; Homo sapiens; RNA-Seq
Probes (photocleaved oligos containing a readout tag sequence identifier and unique molecule identifier) were aspirated by the GeoMx instrument for library preparation. Following collection on the GeoMx instrument, the probes were subjected to polymerase chain reaction amplification during which i5 and i7 indexing sequences were added. Following subsequent pooling, purification, and qualtity control, the library was sequenced on an Illumina HiSeq4000. Spatial Transcriptomics