GSM6481624: Control EE replicate 2; Drosophila melanogaster; RNA-Seq
20,000 PI- GFP+ cells were FACS sorted into 300 μl RNA extraction buffer. The following RNA extraction and amplification steps were carried out using Arcturus PicoPure RNA isolation kit (Applied Biosystems, Cat#KIT0204) and Arcturus RiboAmp HS PLUS RNA amplification kit (Applied Biosystems, Cat#KIT0525) respectively, following the manufacturer's instructions. The NEB Next Ultra II DNA library prep kits (New England Biolabs, cat. no. E7645L) was used for library preparation following the protocal provided by the manufacturer