GSM6499608: IR_17Gy_3M_2; Mus musculus; RNA-Seq
Lungs were resected and single cell suspensions were prepared by enzymatic dissociation (dispase and elastase) and sequential filtering (100 and 40 µm) before red blood cell lysis and cell counting. Single-cell 3'-RNA-Seq samples were prepared using single cell 3'V3 or 3'V3 NextGem reagent kit and Chromium controller (10x Genomics, PN-120237). cDNA quality control was assessed by capillary electrophoresis (Bioanalyzer, Agilent). Single-cell RNA-Seq libraries were prepared using Chromium Single Cell 3'V3 or 3'V3 NextGem Reagent Kit (10X Genomics) according to manufacturer's protocol. Read 1 (26 cycles) corresponds to cell barcode and UMI, Read 2 (98 cycles) corresponds to 3'-end of RNA molecules.