GSM8291538: induced melanocytes from induced pluripotent stem cells, Day 44; Homo sapiens; RNA-Seq
The induced melanocytes were dissociated into single cells using TrypLE digestion. After digestion, the cell suspension was filtered through a 40-micron cell strainer to remove any cell clumps. The cell suspension was centrifuged, and the supernatant was discarded. The cells were resuspended in cryopreservation solution (Stem Cell Baker). Cell counting was performed, and the cell concentration was adjusted to 5-10 million cells per milliliter. The cells suspension was aliquoted into cryovials and stored at -80°C overnight. After overnight storage, the cryovials werer transferred to liquid nitrogen for long-term preservation. The single-cell samples were shipped to BGI Genomics and libraries were prepared according to DNBSEQ protocol.