GSM6965273: Spirosoma montanitarrae DY10T, UVC, 3 hr; Spirosoma montaniterrae; RNA-Seq
The post-radiation cells of UVC were collected by centrifugation (17,500 × g for 5 min) at 5min, 1hr, 3hr and 12hr with washing and stored with RNAlater solution at -80ºC. Total RNA was isolated using the Ambion Ribopure-Yeast RNA kit, according to manufacturer's instructions. Strand-specific RNA-seq library preparation and sequencing was carried out as a paid service by Somagenics Inc., Santa Cruz, California, USA. Paired-end reads (Illumina NextSeq 500 v2, 2 × 150 bp, 3 Gb average output per sample) were obtained from strain DY10T.