GSM6482835: Cortex SCA1 KI [Atxn1 154Q/2Q] Replicate 2; Mus musculus; RNA-Seq
Cerebella and cortex were macrodissected, flash frozen, and kept at -80°C until further processing. The same individual conducted all tissue extractions to eliminate variability in sample collection. RNA was extracted using a Qiagen RNeasy Mini Kit, and DNA was removed with DNase I as described in the manufacturer's protocol. Total RNA was sent to the Yale Center for Genome Analysis for library prepration and sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols.