GSM8332103: DE3; Homo sapiens; RNA-Seq
RNA isolation was performed, as previously described (Thollet et al. 2010; Vendrell et al. 2012), from three independent cell culture replicates of each tested cell line (HEK293 cells stably expressing Halo-tagged ZNF217-WT, ZNF217-ΔE4 or ZNF217-G1013 isoforms; control cells were HEK293 cells stably transfected with pHTN-Halo-CMV empty vector) mRNA was enriched from 200 ng of total RNA, then library preparation was realized with the MGIEasy RNA Directional Library Prep Set (MGI). Quality of libraries were checked by Fragment Analyzer (Agilent) and quantified by Qubit 1X dsDNA HS Assay Kit. Samples were pooled and after circularization in ssDNA, DNB (DNA NanoBalls) were prepared, following the manufacturer protocol.