GSM6482807: Heart_H3K4me3_01dpi_r1; Danio rerio; ChIP-Seq
Nuclei of embryos were disrupted and sonicated to yield fragment size ranging from 200-500bp. Lysates were incubated with the respective antibodies and bound DNA after washes was extracted using Qiagen PCR cleanup kit. Libraries were prepared according to Illumina's instructions accompanying the Ovation Ultra Low Kit