GSM7441724: B13, HFD.ATM.exos, scRNAseq; Mus musculus; RNA-Seq
Epididymal fat was isolated, enzymatically digested in a collagen solution, and after red blood cells were lyzed and a cell vaibility of 80-90% confirmed, stromal vascular cells were used for scRNA-seqencing. Cell suspensions from each mouse were loaded into a single well of a 10× Genomics Chromium Single Cell Controller.