GSM8032842: A549-CBX7-1; Homo sapiens; RNA-Seq
Total RNA was isolated from human cell lines transfected with lentivirus shCBX7 (n=3 for each cell lines) or shCtrl (n=3 for each cell lines). RNA-seq was performed by Genesky Bio-Tech Co., Ltd, Shanghai, China. Quality checks of total RNA were performed using the NanoDrop and Agilent 2100 Bioanalyzer. The QC-qualified mRNA was purified and fragmented, followed by cDNA synthesis. Libraries were prepared using the TruSeq Stranded Total RNA Library Prep Kit and sequenced on the Illumina HiSeq 2500 to generate paired-end reads.