GSM8070161: Treated_day1; Homo sapiens; RNA-Seq
PBMCs were cultured in NK cell media (RPMI 1640 medium containing GlutaMAX, 10% FBS, 1% P-S, 1% MEM Non-Essential Amino Acids Solution and 1% Sodium Pyruvate) without cytokines or with a combination of IL-15 (10ng/mL) and TGF-β (1-10 ng/mL) for 4 days. 4 single cell RNA libraries were constructed using a Chromium controller (10X Genomics Chromium Single Cell 3' V3.1 chemistry). A total of 12 cDNA amplification cycles and 14 cycles of library amplification were used.