GSM7445025: NC.D0_Plate1.B6; Mus musculus; RNA-Seq
Total RNA were directly extracted with lysis buffer inculding Rnase inhibitor from single cell sorted with FACS. RNA sequence library preparation, sequencing, mapping, gene expression, and gene ontology (GO) enrichment analysis were performed by DNAFORM (Yokohama, Kanagawa, Japan). cDNA synthesis and amplification of RNA derived from lysed cell was sequentially conducted in a same PCR tube. After purification, amplified cDNA was validated and quantified. Following tagmentation of cDNA, adapter addition and PCR amplification were conducted using Nextera XT DNA Library Prep Kit (Illumina).