GSM7039250: PBS biol rep1; Mus musculus; RNA-Seq
Cell derived xenografts treated with OMVs@P2O-Ads or PBS were collected and then the total RNA was isolated using RNAiso Plus (TaKaRa). After removal of the remaining genomic DNA,  mRNA was purified from total RNA using polyT and then fragmented with 10× RNA fragmentation buffer (100 mM Tris-HCl, 100 mM ZnCl2 in nuclease free H2O) and the RNA-seq library was constructed using Hieff NGS Ultima Dual-mode mRNA Library Prep Kit for Illumina (Yeasen) according to the manufacturer's protocol, and was sequenced using Illumina NovaSeq 6000.