GSM8256438: RNA-CKO-GO-2W-3; Mus musculus; RNA-Seq
10 oocytes or zygotes from each experimental group were washed for 3 times with 0.2% BSA in PBS and pooled into a 200 μL RNase-free Eppendorf tube. Library construction using smart-seq2 and ERCC spike-in was conducted following established protocol (Picelli, S. et al.  Nat Protoc, 2014. doi:10.1038/nprot.2014.006).