GSM7441326: RNASeq-L2C-G+N-L_rep1; Mus musculus; RNA-Seq
5 embryos were used per group, and three replicates were performed for each group. Embryos were washed three times in 0.5% BSA-PBS solution. cDNA was amplified using the Phusion Hot Start II High-Fidelity PCR Master Mix (Thermo Fisher). Library preparation was performed using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) according to the manufacturer's instructions.