GSM7349365: WSH female arcuate nucleus, MiRIDIAN hsa-miR-211-mimic IVC, rep 2; Rattus rattus; RNA-Seq
Coronal brain sections of the hypothalamus were sliced using a cryostat (-2.3 to -4.5 mm Bregma, using Paxison and Watson coordinates) and the ARC was extracted with a 1 mm disposable Miltex biopsy punch plunger (Bar Noar Ltd). Punches from each hemisphere were immersed in RNA Save (Biological Industries, Kibbutz Beit-Haemek, Israel) for downstream RNA extraction. RNA was isolated using TriReagent (Molecular Research Center, Cincinnati, OH) in DIO/voluntary running experiment. RNA was isolated using RNA/DNA Purification Micro Kit (Cat. 50300) (Norgen BioTek Corporation, Thorold, ON, Canada) in miR-injection experiment. A bulk adaptation of the MARS-Seq protocol was used