GSM7158629: THP1-ELAVL1-KO-1_AZ5576-2; Homo sapiens; RNA-Seq
THP1 Cas9 cells expressing non-targeting control sgRNA (SCR) or sgRNA targeting ELAVL1 (ELAVL1 KO) were harvested, centrifuged (500g at 4°C for 4 minutes), washed in ice-cold PBS and resuspended in 600μL TRIzol. RNA was extracted using the Directzol RNA Miniprep Kit, where an additional DNase I digestion step was performed. QuantSeq 3′mRNA-seq Library Prep Kit FWD for Illumina (Lexogen, Vienna, Austria) 3'mRNA-seq