GSM6756318: S-5LP-1WTInput20191223; Homo sapiens; ChIP-Seq
For HP1g ChIP,  cells were cross-linked with 1% formaldehyde. The crosslink was stopped by adding  2.5 M glycine. Cells were washed with PBS and harvested using ChIP lysis buffer. Cells were then sonicated to obtain fragments (100-500 bp) with Bioruptor Sonicator. Immunoprecipitation was performed with HP1g (17-646). After elution and reversal cross-linking, DNA was purified and sequenced on HiSeq X ten. Construction of ChIP-seq library was performed using VAHTSTM Universal DNA Library Prep Kit for Illumina® V3  (Vazyme).