GSM8332063: 68N8_Rep1; Homo sapiens; RNA-Seq
Cells were collected into tubes containing PBS, centrifuged and lysed with Quick RNA lysis buffer to proceed with RNA extraction with  Quick-RNA MicroPrep (Zymo) according to the manufacturer's instructions. RNA integrity was measured using Bioanalyzer (Agilent). Sequencing libraries were prepared with KAPA stranded RNA-Seq Kit with RiboErase (Kapa Biosystems) according to manufacturer's instructions, and single-end 75 bp sequencing was performed at the USC Molecular Genomics Core, using a NextSeq high output 75 cycle kit (Illumina).