GSM7962454: Gfi1b-GFP+ small intestine scRNA; Mus musculus; RNA-Seq
10x scRNA-seq CD45-EpCAM+GFP+ cells from each mouse specimen were pooled together and submitted for sequencing at the Stanford Functional Genomics Core facility. There, the 10X Genomics Chromium Single Cell 3' v3.0 platform was used to prepare individually barcoded single-cell RNA-Seq libraries following the manufacturer's protocol. Library size was confirmed with Agilent Bioanalyzer High Sensitivity DNA assay.