GSM7519471: P5 parietal leaflet Sox9 epicardial KO 1; Mus musculus; RNA-Seq
Microdissections were performed to isolate individual mitral valve leaflets from P5 mouse hearts. Single leaflets were used as individual samples. Total RNA was purified from each sample using the RNeasy Micro Kit (Qiagen) per manufacturer's instructions. Quality of each total RNA isolation was assessed by Bioanalyzer 2100 (Agilent) and samples with low-quality (RIN<8)  were excluded. RNA samples were submitted to a commercial provider (GeneWiz) for processing by a protocol for low input total RNA