GSM6922380: CP-A TP53-/- C3, control, CTCF; Homo sapiens; ChIP-Seq
Chromatin immunoprecipitation was performed using the SimpleChIP® Enzymatic Chromatin IP Kit (Cell Signaling Technology, catalogue no. 91820S) following the manufacturer's protocol. Chromatin immunoprecipitation was obtained by incubating chromatin at 4°C overnight with either of the following antibodies, at the recommended dilutions: anti-Acetyl-Histone H3 (Lys27) (Cell Signaling Technology, catalogue no. 8173), anti-Tri-Methyl-Histone H3 (Lys9) (Cell Signaling Technology, catalogue no. 13969), anti-CTCF (Active Motif, catalogue no. 61311), anti-Tri-Methyl-Histone H3 (Lys4) (Cell Signaling Technology, catalogue no. 9751), Tri-Methyl-Histone H3 (Lys27) (Cell Signaling Technology, catalogue no. 9733). Library preparation for sequencing from the chromatin immunoprecipitated DNA was performed using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England BioLabs, catalogue no. E7645) following manufacturer's protocol.