GSM7349293: KUNV-infected U87 cells, biol rep 2; Homo sapiens; RNA-Seq
Cells were harvested and homogenized using QIAshredder (Qiagen). RNA was isolated using the RNeasy kit (Qiagen) following the manufacturer's recommended protocols. PolyA-RNA was purified from 5µg total RNA using NEB magnetic mRNA Isolation Kit (NEB, #S1550S) and eluted into a final volume of 25µl. Sequencing libraries were prepared using 9µl of isolated mRNA and ScriptSeq RNA-Seq Library Preparation Kit (Epicentre, SSV21124).