GSM6533953: P7-Retina-EC-ATACseq-CTR-rep1; Mus musculus; ATAC-seq
At P7 mice were sacrificed and Retina were dissected followed by digestion with Enzyme solution (Collagenase I 1mg/ml, DNAse I 0.1 mg/ml in HBSS w/o Mg/Cl) for 45 min at 37°C with agitation. The digestion was stopped by addigng Basal Medium (DMEM high glucose, 20% FCS, 1xP/S, 1xGlutamin, 25mM HEPES) and filtering the cell suspension trough a 70 µm cell strainer. After centrifugation the cell pellet was resuspended in 1 ml Basal medium and bound with CD31-coupled magnetic Dynabeads (eBioscience #17031180) for 30 min at RT with agitaion. After washing 5 times on a magnetic rack, EC were counted and max 50.000 cells were used for ATAC. tagmented DNA; Illumina Nextera Tagmentation kit; IDT-ILMN Nextera DNA UD Indexes (384) Plate C;