GSM8332636: HUVEC,MeAIB1,rep1,12h; Homo sapiens; RNA-Seq
Total RNA was isolated using TRIzol (Invitrogen) according to the manufacturer's instructions.Total amounts and integrity of RNA were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). RNA libraries for RNA-seq were prepared using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® following manufacturer's protocols.