GSM7244876: term-seq_∆rnr_R2; Campylobacter jejuni; RNA-Seq
total RNA was treated with DNase I to digest genomic DNA and ribosomal RNA was depleted 3'Term protocol: First, the 5' Illumina sequencing adapter was ligated to the 3'-OH of the RNA molecules and cDNA synthesis was performed using M-MLV reverse transcriptase. Then, first-strand cDNA was fragmented and the 3' Illumina sequencing adapter was ligated to the 3' ends of the single-stranded cDNA fragments. Finally, the 3' cDNA fragments were PCR-amplified, purified with the Agencourt AMPure XP kit (Beckman Coulter Genomics), and analyzed by capillary electrophoresis.