GSM6533676: hypoABF5-3_scRNA-seq; Mus musculus; RNA-Seq
Freshly dissected hypothalami were dissociated with papain (Worthington, Lakewood, NJ, USA) for single cell isolation (Brewer and Torricelli, 2007).  Cells were suspended in 0.01% BSA-PBS at a final concentration of 100 cells/μl for as previously described (Arneson et al., 2018). Single cells were barcoded using single-cell transcriptomes attached to microparticles (STAMPs) followed by generating cDNA library with the Drop-seq protocol by Macosko et al 2015 (Macosko et al., 2015).