GSM8291854: LF1 cells, quiescent, replicate 2, Hi-C; Homo sapiens; Hi-C
All conditions (proliferating, quiescent, RS) were crosslinked with 1% PFA at room temperature for 10 minutes while still attached to cell culture plates. Crosslinking was quenched with glutamine. Cells were washed with PBS three times before being scraped into a microcentrifuge tube. To perform Hi-C the Arima Genomics Hi-C kit was used.