GSM6718977: WTCPM-dex-3-rep2; Mus musculus; RNA-Seq
The lung samples from 16 mice  belonging to four groups including WTCPM, WTCPM+ros, WTCPM+dex, and control (n = 4), werethen processed for RNA extraction and high-throughput sequencing. TRIzol (Invitrogen) was adopted to extract the RNA based on the commercial protocol. The RNA quality and purity for each sample were assessed by Nanodrop 1000 spectrometer (Thermo Fisher Scientific, Wilmington, DE). DNA contamination was eliminated by following the manufacturer's instructions for using a DNA-free kit (Invitrogen).  Next, an Illumina HiSeq 4000 system (NYU Genome Technology Center) was used to perform sequencing  of 1 × 50 paired-end reads with 186,512 transcripts. Illumina HiSeq 4000 system (NYU Genome Technology Center)