GSM8034928: 94_primary_CD64_long_Tala_019; Homo sapiens; OTHER
Slide-mounted FFPE TMA was processed for antigen retrieval using a heat induced epitope retrieval protocol for 20 minutes followed by a 5-min wash with 1μg/mL proteinase K solution. TMA was next incubated overnight with GeoMx RNA detection probes containing-photocleavable oligos Sequencing libraries were generated by PCR from the oligos and sequenced using Illumina NovaSeq according to the manufacturer's protocol