GSM7110413: dsrct PDX Tr (PDX_3C); Homo sapiens; RNA-Seq
Total RNA extracted from untreated PDXs and PDX exposed to one round of treatment with irinotecan or trabectedin as single agent or in combination was processed with an RNeasy mini kit (Qiagen) in accordance with the manufacture's protocol. RNA was processed according to standard NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® protocol and mRNA was selected with oligo-dT beads. All the RNA libraries were generated starting from 800ng total RNA. Libraries were quantified and quality checked on an Agilent 4200 TapeStation system (High Sensitivity D5000), and then sequenced on an Illumina NovaSeq platform with paired-end reads 150bp long, with a depth of 27 million cluster/sample.