GSM7918279: SNU1079, ARV825, 48h; Homo sapiens; ChIP-Seq
After harvest, cells were crosslinked with 1% formaldehyde for 10 min at room temperature and the reaction were quenched with 125 mM glycine. After chromatin were fragmented to 200 to 600 bp by sonication, chromatin templates from 10 million cells were used for ChIP experiment. Samples were immunoprecipitated with 2μg of H3K27ac (Abcam, #ab4729) antibody overnight at 4 °C followed by washing steps. After reverse cross-linking, the ChIP DNA fragment were purified and repaired using End-It™ DNA End-Repair Kit followed by treatment with Taq polymerase to generate single-base 3´ overhangs used for adaptor ligation. Following the ligation of a pair of Illumina adaptors to the repaired ends, the ChIP DNA were amplified using the adaptor primers. ENCODE guidelines were used to maintain the quality of all data sets, including the read depth.