GSM8292125: input_1; Homo sapiens; ChIP-Seq
Blood monocytes from 3 individual donors were stimulated for 20 min in the presence of 5 ng/mL of IL4 in complete medium, in the presence of 8 microM SR1 (Cayman chemicals) or 60nM FICZ (Enzo Life Sciences) . Chromatin immunoprecipitation was performed from 80 millions monocytes as starting material, using the ChIP-IT High Sensitivity kit (Active Motif) following manufacturer's instructions. Chromatin was immunoprecipitated using an antibody against STAT6 (clone 7D3, ThermoFisher).. Libraries were prepared according to Illumina's instructions accompanying the TruSeq ChIPSeq Library Prep Kit (Illumina). 1 ng of DNA was used for each sample. Sequencing was performed in 1 sequencing unit of NovaSeq 6000 (Illumina) (100-nt593 length reads, paired end).