GSM8120860: KO, Saline, 12mo, rep 3; Mus musculus; RNA-Seq
Total RNA was extracted from frozen brain powder using the Qiagen RNeasy plus kit (Qiagen, 74034). Quality and quantity were assessed with a 4200 TapeStation System (Agilent). Next generation sequencing libraries were generated with the QuantSeq 3' mRNA-seq Library Prep Kit FWD for Illumina (Lexogen A01173), with the UMI second strand synthesis module in order to identify and remove PCR duplicates. Library quantity and quality were assessed with the 4200 TapeStation system (Agilent). Libraries were pooled in equimolar ratios for sequencing.