GSM7349775: Patients 048, 049, 050; Homo sapiens; RNA-Seq
White blood cells from convalescent COVID-19 patients were isolated from whole blood samples. Monocytes and Tregs were labeled with appropriate fluorescent antibodies and sorted using FACS. Patient cells were labeled with a hashtag barcode oligo and libraries were prepared per manufacturer's instructions. Briefly, Cells were loaded onto a 10X Chromium controller, and librray was constructed using 10X chromium Next GEM Single Cell 5' Library and gel bead kits (v1.1)