GSM7109902: NK cells, LD IL-15, rep2 [ATAC-seq]; Mus musculus; ATAC-seq
Assay for transposition of active chromatin sequencing (ATAC-Seq) was performed as previously published (Buenrostro, 2013) on single cell suspensions. Aliquots of approximately 75,000 cells (LD/HD IL-15) were used as input per biological replicate. Transposase reaction occurred at 37C for 30 minutes. DNA was purified with the Qiagen MinElute PCR Purification kit (Qiagen). Libraries were amplified and barcoded using the NEBNext Ultra 2xMastermix (NEB) and Illumina UD Indexes (10 bp).The libraries were quantified using the universal library quantification kit (KAPA Biosystems). The product was purified using Qiagen MinElute PCR Purification kit, followed by AMPure bead selection. ATAC-Seq libraries profiles were visualized using High Sensitivity D1000 ScreenTape on the Tapestation (Agilent).