GSM6755734: carnation petal, ethylene treatment, 24h, rep2, IP [IP-E24h-2]; Dianthus caryophyllus; ChIP-Seq
petals were fixed with 1% formaldehyde for 30 minutes and sonicated into DNA fragments. 6% of the sonicated chromatin was saved as the input. The left samples were subsequently incubated with 2 µl of antibodies of anti-H3K4me3 (abcam ab8580) overnight at 4°C with gentle agitation. Immunoprecipitated DNA was used to construct sequencing libraries following the protocol provided by the I NEXTFLEX ChIP-Seq Library Prep Kit for Illumina Sequencing（NOVA-5143-02，Bioo Scientific） and sequenced on Illumina NovaSeq 6000 with PE 150 method.