GSM7158930: Lev-diazirine-comp-1; Homo sapiens; RNA-Seq
To isolate total cellular RNA, cells were lysed with 6 mL of Trizol LS reagent according to the Supplementary Information. RNAs were then purified with a Zymo Quick-RNA Midiprep kit according to the manufacturer's protocol. To enrich poly(A)+ transcripts, a 250-µg aliquot of total cellular RNA was diluted to 600 µg/mL with RNA storage buffer and then purified with Poly(A)Purist MAG kit according to the manufacturer's protocol. The captured RNAs were further purified with RNeasy Mini columns according to the manufacturer's protocol, and stored as 500-ng aliquots at -80 ˚C. RNA libraries for RNA-seq were prepared using a customized protocol developed in our lab (bioRxiv 2022.07.18.500496; doi: https://doi.org/10.1101/2022.07.18.500496)