GSM8119610: Emue_MARSseq; Ephydatia muelleri; RNA-Seq
To collect cells for MARS-seq libraries, 7 days post hatching sponges were fed with 0.5 µm fluorescent carboxylate-modified FluoSpheres (Invitrogen, F8813). Animal tissues were dissociated, choanocytes were FACS sorted into four 384-well MARS-seq plates. The MARS-seq libraries were prepared as described in Sebé-Pedrós et al. 2018 (PMID: 29942020).