GSM8257027: HTO library for CEL190419LH_GEX; Homo sapiens; RNA-Seq
Media was aspirated and cells were washed twice with PBS. Cells were incubated in TrypLE Express for approximately 5 minutes until detached from the well surface, then gently titurated before enzyme neutralization (10% FBS in PBS). Cells were spun at 300xG for 5 minutes and then resuspended in PBS for library preparation (LH2400, LH2401, LH7200, LH7201) or HTO staining (CEL190419LH) following manufacturer protocol (TotalSeq-A, BioLegend). LH2400, LH2401, LH7200, LH7201: Libraries were prepared following manufacturer protocol (10X Genomics Single Cell 3' v2). CEL190419LH: Libraries were prepared for both single-cell RNA and antibody feature following manufacturer protocol (10X Genomics Single Cell 3' v3).