GSM7440075: FAW R1; Spodoptera frugiperda; miRNA-Seq
Total RNA extraction using the Qiagen miRNeasy Mini Kit, according to the manufacturer's instructions (Qiagen, Valencia, CA) Qualified total RNA was used to construct sRNA libraries using the Truseq small RNA sample Pre Kit (Illumina).Illumina sRNA libraries were constructed from each pool, and 50-bp single-end (sE50) sequence read data were generated on an Illumina Hiseq 4000 at the Research Technology support Facility (Michigan state University, East Lancing, MI, UsA). Clean reads were obtained from the raw data after processing adapters, low-quality reads, and sequences of fewer than 18 nucleotides using cutadapt v.3.471.