Illumina HiSeq 4000 sequencing; ROBO3s: a novel ROBO3 shot isoform promoting breast cancer aggressiveness
HCC1806 cells were treated with siRNA against ROBO3s in 6-well plates for 72 hours in biological triplicates. Total RNA was extracted from HCC1806 cells with Qiazol (Qiagen) following the manufacturer's recommendations.  mRNA sequencing libraries were generated with the Illumina Trueseq v2 Kit according to the manufacturerâ€™s instructions. The size of the generated libraries was estimated on High Sensitivity DNA chip (Agilent) using a Bioanalyser 2100 (Agilent).