GSM8427622: Blood; Homo sapiens; RNA-Seq
Blood was collected by venipuncture from a healthy donor and PBMC were isolated with a ficoll gradient. T cells were isolated by negative magnetic selection using the total T cell isolation kit (STEMCELL technologies). T cells were then rested for 2 hours in RPMI-10 at 37C and subsequently used as input in a transwell-based chemotaxis assay using h4CysCXCL17 300nM as chemoattractant as described above. Migrated cells were recovered from 9 independent transwell wells and along with the corresponding input samples, were separately hash tagged and finally mixed together in preparation for a single 5' 10x Genomics scRNAseq capture. Library was constructed using the 10x Genomics 5' v3 kit according to the manufacturer's instructions.