Single cell RNAseq of Neuronal-derived haploid and diplod human embryonic stem cells
Cells were washed with PBS and trypsinized Haploid and diploid neurons were separated into droplet emulsion using Chromium X (10x genomics). scRNAseq libraries were prepared and barcoded with UDIs using Chromium Next GEM Single Cell 3â€™ Kit v3.1 (10X Genomics), according to the manufacturer protocol.