GSM7037652: 6_MZ10_A_PB_CD8T; Homo sapiens; RNA-Seq
PB CD56dim and MB uNK cells as well as PB CD8+ TEM and MB CD8+ TRM cells were pre-gated on alive CD45+CD14-CD15-CD19- cells and sorted directly into 300 ul of RLT plus buffer (QIAGEN) in a sterile ribonuclease- and DNase-free 1.5 ml Eppendorf tube and frozen on dry ice immediately after sorting. RNA was isolated with the RNeasy Plus Micro Kit (QIAGEN). Extracted RNA was quality tested with a Bioanalyzer Pico kit (Agilent), before cDNA was generated with the Smart-seq v4 (Clontech). Next, indexed cDNA libraries were normalized, combined, and pool-sequenced on the Illumina NextSeq 550 for a 75-cycle v2.5 sequencing generating 150–base pair paired-end reads.