GSM8162589: HX Female KO rep.7; Mus musculus; RNA-Seq
Qiagen RNAeasy minikit according to the manufacturer's protocol INCPM Bulk-MARSSeq: RNA-seq libraries were prepared at the Crown Genomics institute of the Nancy and Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science. A bulk adaptation of the MARS-Seq protocol (Jaitin et al., Science, 2014; Keren-Shaul et al., Nature Protocols, 2019) was used to generate RNA-Seq libraries for expression profiling of XXX. Briefly, (30 ng of input) RNA from each sample was barcoded during reverse transcription and pooled. Following Agencourct Ampure XP beads cleanup (Beckman Coulter), the pooled samples underwent second strand synthesis and were linearly amplified by T7 in vitro transcription. The resulting RNA was fragmented and converted into a sequencing-ready library by tagging the samples with Illumina sequences during ligation, RT, and PCR.  Libraries were quantified by Qubit and TapeStation as well as by qPCR  as previously described (Jaitin et al., Science, 2014; Keren-Shaul et al., Nature Protocols, 2019). Poly-A and 3' UTR targeting