GSM6481506: MM6, shNS, mRNA, rep1; Homo sapiens; RNA-Seq
For input samples, RNA was isolated using Trizol reagent. A total amount of 1 µg RNA per sample was used as input material for the RNA sample preparations. For ribo-seq samples, ribosome profiling was performed using Epi™ Ribosome Profiling Kit (Epibiotek, R1814). Subsquently, RPFs (ribosome-protected RNA fragments) was extracted using RNA clean&ConcentratorTM-5 kit (ZYMO, R1016). For input samples, VAHTS Stranded mRNA-seq Library Prep Kit for Illumina V2 (Vazyme Biotech, NR612-02) was used for library preparation according to the instructions. For ribo-seq sample, EpiTM RiboRNA Depletion Kit (Human/Mouse/Rat) (Epibiotek, R1805) was used for rRNA depletion. Sequencing libraries were constructed using QIAseq miRNA Library kit (QIAGEN, 1103679).