GSM8255977: B2; Homo sapiens; RNA-Seq
Total RNAs were extracted  using TRIzol Reagent (Invitrogen, cat. NO 15596026）following the methods by Chomczynski et al (DOI:10.1006/abio.1987.9999).2 μg total RNAs were used for stranded RNA sequencing library RNA libraries for RNA-seg were prepared using VAHTS Stranded mRNA-seq Library Prep Kit for Illumina V2 (Vazyme Biotech, NR612-02)  following manufacturer's protocols.