GSM7958838: U2OS, pLVX-NAB2-STAT6, 2 days doxycycline, ATAC, biol rep2; Homo sapiens; ATAC-seq
Omni ATAC-seq was performed on both control and 2 days 1ug/mL doxycycline treated U2OS pLVX-NAB2-STAT6 cells according to Corces et al 2017. Briefly, 50,000 cells were washed in  ATAC-resuspension buffer (RSB)  with 0.1% Tween-20 and then lysed by incubated on ice for 3 minutes in RSB with 0.1% NP-40 and 0.1% Tween-20. Lysis was washed out in RSB 0.1% Tween-20 and nuclei was pelleted by centrifugation at 600g/4°C/5 minutes. Nuclei were then incubated in transposition mixture for 30 minutes at 37°C while shaking at 1000rpm. DNA was then purified using Zymo DNA clean and concentrator-5 Kit (cat# D4014). DNA was then amplified for 5 cycles with ATAC index primers and NEBNext Ultra II Q5 Master Mix (NEB #M0544). Additional amplification cycles were then determined by qPCR using 5uL of original amplification with PerfeCTa SYBR Green FastMix Reaction Mixes (Quantabio 95072-012). After additional amplification cycles with remaining 15uL DNA was then purified using Zymo DNA clean and concentrator-5 Kit (cat# D4014) and quantified by QUBIT. Omni-ATAC-seq (Corces et al. 2017)