GSM6965454: U937 cells, wild type, ATAC-seq Replicate 1; Homo sapiens; ATAC-seq
Cells were lysed using cold lysis buffer. Nuclei were spun at 500g for 10 min using a refrigerated centrifuge. The pellet was resuspended in the transposase reaction mix. Directly following transposition the sample was purified using a Qiagen MinElute kit. Libraries for ATAC-seq were prepared using Nextera DNA Sample Preparation kit (Illumina)