GSM8292114: S4; Mus musculus; RNA-Seq
Mice were anesthetized with chloral hydrate (4.2%, 0.01 mL/g) and underwent transcardial perfusion with 40 mL of pre-cooled 0.9% NaCl, right hippocampus were isolated. Total RNA was extracted and mRNA was isolated using magnetic beads with oligo-dT, the mRNA was then segmented, the cDNA were synthesized and end repaired. A base was added to the 3'end and the segment was connected to the sequencing connector, which was then purified and amplified, the sequencing library was purified by magnetic beads. Qubit was used to detect the library concentration, and agilent fragment analyzer was used to detect the library fragment length. RNA-Libraries for RNA-Seq were prepared using SMARTER mRNA-Seq Libraries prep kit following manufacture's protocols