GSM8292052: MAC+ET-1 treated HG-SOC cells rep3; Homo sapiens; RNA-Seq
Total RNA was isolated using the RNeasy Quick Start kit (Qiagen), according to the manufacturer's protocol. Quantity and quality of the starting RNA were checked by NanoDrop (Thermo Scientific). Before starting with library prep, RNA quality was determined running samples on Tapestation 4100 (Agilent). Only RNA samples with RIN above 7 were used in the next steps. To generate the NGS libraries, we exploited the TruSeq Stranded mRNA (Illumina) kit, starting from 100ng of total RNA. Libraries were barcoded, pooled, and sequenced on an Illumina NextSeq 500 sequencing system, in order to obtain for each sample ~ 20-30M single-end reads, 100 nt long.