GSM7698245: Plate numbers P189 and P207; Mus musculus; RNA-Seq
Cells from E14.5 fetal liver were immunolabeled and subjected to fluorescence activated cell sorting to purify Lin-Sca-1+cKit+CD150+CD48- HSCs. Single cells were deposited into wells of a 384-well plate. Single cell libraries were generated as follows: Reverse transcription was conducted with a CEL-Seq2 primer containing T7 promoter, partial Illumina 5' adaptor, unique molecule identifier (UMI), 8bp barcode and polyT stretch followed by second strand synthesis, sample pooling, In vitro transcription, aRNA fragmentation and a final PCR to incorporate full length Illumina P5 and P 7 adaptors.