GSM6923169: Thermoneutral 2; Mus musculus; RNA-Seq
Fat tissues frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were constructed by TruSeq® RNA LT Sample Prep Kit v2 (illumina, USA) following the manufacturer's protocol and quantified by Qubit (invitrogen, USA) for cluster generation.