GSM6755743: Nascent RNA, chromatin-released, pulse-chase time point 10 min; Homo sapiens; RNA-Seq
Chromatin fractionation was done at 0.5 M urea and RNA was extracted from the chromatin-associated and nucleoplasmic fraction with acidic phenol (pH 4.5) and acidic phenol/chloroform. RNA libraries for RNA-seq from 4-SU-labeled purified RNA were prepared using the TrueSeq Stranded mRNA Library Preparation Kit (without RiboZero).