GSM6533753: BLAS cells expressing mutated Tat [blas_tatc22g_2]; Homo sapiens; RNA-Seq
Total RNA from BLAS cells was isolated using the NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany) following manufacturer's instructions. 3 ug of total RNA was sent for the construction of sequencing libraries. RNA-seq libraries were prepared using the Agilent SureSelect Automated Strand-Specific RNA Library Prep using the manufacturer's protocol.