GSM6533797: Control Placenta Site Sample D2; Homo sapiens; RNA-Seq
The placenta biopsies was mechanically disassociated and enzymatically digested by incubating in digestion media of 2.5% trypsin, 300 U/mL collagenase, and 200 ug/mL DNAse in PBS for 90 minutes at 37oC in 5% CO2. Red blood cell lysis buffer was added to eliminate red blood cells. The cells were strained through a 100uM nylon mesh strainer. The cells were frozen for later use. At time of processing, the frozen cells were thawed and resuspended in an ideal cell concentration of 700-1200 cells/uL for single-cell sequencing. scRNA-seq libraries were generated using 10X Genomics Chromium Single Cell 3' Library & Gel Bead Kit v3. Cells were loaded accordingly following the 10X Genomics protocol with an estimated targeted cell recovery of 6000 cells.