GSM8070305: BMDM, Wild-type, LPS + CCM, rep2; Mus musculus; RNA-Seq
Cells were lysed in Trizol reagent and RNA extracted using chloroform, followed by Zymo RNA Clean & Concentrator. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection.