RNA sequencing from publication: Ki-67 is necessary during DNA replication for forks protection and genome stability
Ki-67 was degraded in cells arrested in prometaphase and released in thymidine for 18h. Then the sample was collected Total RNA extraction was performed with the Monarch Total RNA Miniprep Kit (New England Biolabs, Hitchin, UK) according to the manufacturer's protocol. Libraries generated with TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) according to the manufacturer's protocol.