GSM6755656: 4393_6_TL608_cDC2_GTGAAAC; Homo sapiens; RNA-Seq
Total RNA isolation was performed according to manufacturer's protocol using the RNeasy MinElute Cleanup Kit (Qiagen). Strand-specific cDNA libraries were generated using the TruSeq Stranded mRNA sample preparation kit (Illumina) according to the manufacturer's protocol. The libraries were analyzed for size and quantity of complementary DNAs (cDNAs) on a 2100 Bioanalyzer using a 7500 chip (Agilent), diluted and pooled in equimolar ratios into a multiplex sequencing pool. The libraries were sequenced as 65 base single reads on a HiSeq2500 with V4 chemistry (Illumina).