GSM6717088: 423, Hypothalamus, WT, Male; Mus musculus; RNA-Seq
RNA was harvested using RNeasy Mini Kit and RNeasy Micro Kit (Qiagen), following manufacturer's protocols. For each sample of hypothalamus and PFC, 1000 ng and 500 ng were used as input quantity, respectively. RNA libraries for RNA-seq were prepared using paired-end TruSeq® Stranded mRNA Sample Preparation kit (Illumina, San Diego, Ca, USA), following manufacturer's protocols.