GSM7519507: Bio Rep1_Wildtype1_No ABA treat; Arabidopsis thaliana; RNA-Seq
Total RNA was extracted from ABA-treated seedling including control sample (no ABA-treated) using Directzol RNA miniprep (Zymo Research, USA) 1µg of DNA-free total RNA for Isolate mRNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490) and followed the procedure by NEBNext UltraII Directional RNA library Prep Kit for Illumina (NEB, E7765) with slightly modified condition such diluted adaptor (1:5), extended incubation of ligation for 2 hours at room temperature, and 7 PCR cycles.