GSM8291482: wild-type mice_2, 32-week-old; Mus musculus; RNA-Seq
Collect 100mg of gastrocnemius muscle from anesthetized mice and promptly freeze the muscle tissue in liquid nitrogen. Then samples were sent on ice to Novogene Technology Co., Ltd for immediate downstream RNA sequencing. In brief, following RNA isolation with Trizol (catalog #, check with Novogene), a total amount of 2 μg RNA per sample was used as input material for library preparations. Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (#E7530L, NEB, USA) following the manufacturer's recommendations and index codes were added to attribute sequences.