GSM6959136: F4/80lowTAMs_melanoma-day21_rep3; Mus musculus; RNA-Seq
Sorted cells were spinned down and lysed by resuspending in 1mL RLT buffer and RNA was extracted using RNeasy mini plus kit (Qiagen). 200ng-1ug of Purified total RNA suspended in nuclease-free H2O (15-25ul) was submitted per sample. RNA quality control protocol: RNA had an OD260/280 ratio of 1.8-2 and an OD260/230 ratio of 1.8-2. RNA integrity was verified in an Agilent Technologies 2100 Bioanalyzer through an Eukaryote Total RNA Pico Series II assay. For this poly_A-based library preps, high-quality RNA had a bioanalyzer RIN score of 7 or higher. https://www.illumina.com/systems/sequencing-platforms/miseq.html https://medicine.yale.edu/keck/ycga/sequencing/illumina/9_21599_284_12501_v1.pdf