GSM6755943: E15_5_KO scRNA-seq; Mus musculus; RNA-Seq
Pancreatic tissues of E13.5 and E15.5 embryos were collected, disassociated by TrypLE™ Express (ThermoFisher 12605010), labelled with EpCAM and then purified by flow cytometry. Individual libraries were diluted to 4 nM and pooled for sequencing. At last, pools were sequenced on Illumina novaseq6000 with 150 bp paired end reads. Individual libraries were diluted to 4 nM and pooled for sequencing. At last, pools were sequenced on Illumina novaseq6000 with 150 bp paired end reads