GSM7036806: Donor 1, Septum, scATAC-seq; Homo sapiens; ATAC-seq
scATAC-seq libraries were prepared according to v1 of the manufacturer's guidelines for the Chromium Next GEM Single Cell ATAC Reagent Kits (10X Genomics), with the modification that we started from nuclei that were isolated. Between 9,300 and 25,000 nuclei were tagmented using Transposition Mix (10X Genomics) at 37°C for 1 h and loaded on the Chromium controller. We targeted between 6,000 and 10,000 nuclei for library preparation. QC was performed on final ATAC-seq libraries using a Qubit Fluorometer and an Agilent 2100 Bioanalyzer.