GSM8121319: IEL WT CD69+CD103+ rep3; Mus musculus; RNA-Seq
Donor P14 T cells were FACS sorted based on CD8, CD45.1 and CD45.2. RNA was extracted from sorted cells using the Quick-RNA™ MiniPrep, according to manufacturer's instructions (Zymo Research). Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample