GSM6578708: Exp2_RluA_2_rep1_CMC; Drosophila melanogaster; OTHER
The flies were then snap frozen in liquid nitrogen and kept at -80°C for 3 days until sufficient number of flies from each genotype had been collected. The frozen flies were then decapitated and 100 heads per replicate homogenized in TRIzol reagent (Invitrogen). Total RNA was prepared according to the manufacturers' recommendations. Polyadenylated RNA was enriched from total RNA using two rounds of Oligo(dT) dynabeads (Invitrogen) according to manufacturer's protocol. To each polyA-RNA sample, spike-in RNA (~3ug) was added and then the samples were split into two equal samples for CMCT or mock treatment.