GSM8070689: 6W-2mm10_asis; Mus musculus; RNA-Seq
At 4 (CPZ) and 6 weeks (Control and Recovery), mice were euthanized by carbon dioxide (CO2) inhalation, and their brains were subsequently dissected. Each brain was sagittally cut into left and right hemispheres. One hemisphere was then further divided into three coronal segments, which were embedded in OCT for snRNA-seq. snRNA-seq was performed using the 10X Genomics Chromium Next GEM Single Cell 3' Kit v3.1 (PN1000268), Chromium Next GEM Chip G (PN1000127), and Dual Index Kit TT Set A (PN1000215). Nuclei suspensions were loaded onto the 10X Chromium instrument with the aim of recovering 5000 nuclei per sample. Libraries were sequenced on an Illumina NovaSeq 6000 S4 flowcell using paired-end sequencing with the following parameters: Read1-28bp, i7-10bp, i5-10bp, Read2-90bp.