GSM8120441: anti-PD-1 + control anti-KLH treatment at Week 4, mouse KWM_a; Mus musculus; RNA-Seq
RNA isolation - Total RNA from tumors was isolated using Trizol and was purified using an RNAeasy kit (Qiagen). Sequencing - Total RNA was then prepared for sequencing using the TailorMix Directional mRNA sample preparation kit (SeqMatic) and sequenced on Illumina NovaSeq V1.5 SP (100bp single-end reads and an average of 40 million reads were generated for each sample).