GSM7864804: M11; Homo sapiens; RNA-Seq
Kidney tissue was obtained from a clinical biopsy, placed in Michels solution and frozen on OCT within 24 h. 10 um of cross-sectional biopsy tissue was cut on a cryotome and placed in PicoPure RNA extraction buffer. RNA was extracted according to protocol. Ribosomal RNA was removed using RiboGone – Mammalian Kit. SMARTer® Universal Low Input RNA Kit for Sequencing, shearing by Covaris, Ion Plus Fragment Library Kit Illumina HiSeq 4000 RNA seq, paired-end, 30 million reads per sample library