GSM6719939: Single healthy control; Homo sapiens; RNA-Seq
RNA was isolated using RNAiso Plus (Takara) followed by DNase treatment from MasterPure complete DNA and RNA purification kit (Lucigen). 500ng RNA used to prepare library using TrueSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold (Illumina Inc.) as per manufacturer's protocol librabry sample was diluted to 400pM and loaded onto NovaSeq 6000 machine using SP flow cell (Illumina) for sequencing