GSM7519753: HemECs, shMIR4435-2HG-2; Homo sapiens; RNA-Seq
Total RNA was extracted using RNAiso Plus reagent (Takara, Tokyo, Japan). 2 μg of  total RNA was used for the construction of  sequencing labraries. The libraries were constructed using TruSeq Stranded mRNA LTSample Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer's instructions.