GSM8292466: Control1, ST; Homo sapiens; OTHER
FFPE ovary sections (5 microns) were mounted onto a positively charged slide followed by staining with hematoxylin and eosin. Slides were mounted in 70% glycerol and brightfield images were taken using microscope.  The tissue sections were transferred immediately onto 6.5 × 6.5mm or 11mm x 11mm capture areas on a Visium gene expression slide using Visium CysAssist (10x Genomics). The tissue sections were then subjected to permeabilization followed by reverse transcription for cDNA synthesis. Subsequently, the cDNA amplification was performed, and spatial libraries were constructed. The libraries were pooled and paired-end sequencing was performed on S2 flow cell using an Illumina NovaSeq 6000 platform. Spatial transcriptome sequencing libraries from ovarian tissue were constructed using 10x Genomics Visium spatial gene expression slide & reagent kit