GSM7886758: Nasal mucosa, NS, Rep2, A22_2; Mus musculus; miRNA-Seq
Total RNA was extracted using TriQuick Reagent (Solarbio, China) from nasal mucosa of mice. 2 ug of total RNA was used for the the construction of sequencing libraries. For miRNA, all samples were resuspended in 8 µl water and 6 µl RNA were subjected to NGS library preparation using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, Ipswich, USA) with minor modifications to the manufacturer's protocol.