GSM7110518: hepatocyte, lipid treated, replicate B [6_hep_B_lipid]; Mus musculus; RNA-Seq
Total RNA was isolated from treated hepatocytes using a PureLink RNA Mini kit with on-column DNAse digestion (Invitrogen). All samples met quality control of RIN > 8.0 using a Bioanalyzer. 500ng total RNA was used via the stranded mRNA ligation kit (Illumina) as input to an mRNA capture, cDNA synthesis, and standard Illumina library prep prior to sequencing.