GSM8069492: ATAC_IKZF4-Ex2-sg1-C29; Homo sapiens; ATAC-seq
A total of 50,000 cells per clone were harvested and lysed in Base Buffer (10mM Tris-HCl, 10mM NaCl, 3mM MgCl2, pH 7.4) with 0.1% NP-40 (Sigma/Roche Cat# 11332473001), 0.1% Tween-20 (Sigma-Aldrich Cat# 11332465001) and 0.01% Digitonin (Promega Cat# G9441). After pelleting the cells, transposition reaction was performed using Nextera DNA Library Prep Kit (Illumina, Cat# FC-121-1030) and amplification of transposed DNA fragments was performed using Nextera Index Kit (Illumina, Cat# FC-121-1011) following manufacture's protocols. Sample purification was achieved by using Agencourt AMPure XP beads (Beckman Coulter, Cat# A63881). Sample yield was determined using Qubit High Sensitivity dsDNA (ThermoFisher, Cat# Q32851) and DNA integrity was assessed by D1000 High Sensitivity Tape Station (Agilent TapeStation system).