GSM6965918: RHN418; Homo sapiens; RNA-Seq
RNA was harvested by adding 1ml of Trizol reagent (Life technologies, 15596026), isolated with 100% chloroform, precipitated with 100% isopropanol follow by 80% ethanol and purified with Qiaquick RNA purification kit (Qiagen, 28106). Libraries were prepared for RNA-seq using the TruSeq Stranded Total RNA Library Prep Kit (Illumina, catalog no. RS-122-2303). Samples were sequenced at the Genome Institute Singapore (GIS) sequencing core on an Hiseq 2500 Sequencing System (Ilumina) generating paired-end, 100-bp reads with an average of 20-30 million reads per sample.