GSM8257021: Geriatric TA Muscle, 5dpi; Mus musculus; RNA-Seq
Slide-seq Spatial Transcriptomics libraries for young and geriatric TA muscle sections were prepared using the Curio Seeker Spatial Transcriptomics kit (Curio Biosciences), following the manufacturer's instructions. Briefly, fresh-frozen tissue was mounted on 3mm x 3mm Curio Seeker tiles, followed by RNA hybridization, reverse transcription, enzymatic digestion, and bead detachment from the glass tiles. This was followed by second strand cDNA synthesis and amplification. The cDNA ibraries were prepared with the Nextera XT DNA sample preparation kit following Curio instructions. The samples were then pooled and sequenced on an Illumina NextSeq 2K system with a P2 flow cell and a 100-cycle kit, with read configurations as follows: Read 1 = 50 bp, Read 2 = 72 bp, Index 1 = 8 bp, and Index 2 = 8 bp. Spatial transcriptomics