GSM6965846: pool Cells 14/15A pool number 14; Mus musculus; OTHER
Genomic DNA extraction of Day 0, in vitro and in vivo samples was carried out using Qiagen Gentra Puregene Tissue Kit. A two-step PCR was carried out to amplify shRNA sequences from the genomic DNA samples. Sequencing adapters and multiplexing barcodes were incorporated into the PCR primer. PCR products were purified using Qiagen MiniElute PCR Purification Kit and DNA concentration was determined using Bioanalyser.