GSM8291876: copper treatment_rep1; Vibrio parahaemolyticus; RNA-Seq
RNA was extracted using an Eastep Super Total RNA Isolation Kit (Promega, Shanghai, China), according to the manufacturer's instructions. RNA integrity and concentration were evaluated by gel electrophoresis and spectrophotometric analysis, respectively. Subsequent library preparation was performed using 1ug total RNA. Libraries with different indexes are then multiplexed and loaded onto the Illumina X Ten instrument according to the manufacturer's instructions (Illumina, San Diego, CA, USA) or according to the manufacturer's instructions (MGI, Shenzhen, China).