GSM6718774: Faa1 knockout, rep4 [20191030_Jose_9]; Yarrowia lipolytica; RNA-Seq
The WT and Δfaa1 Y. lipolytica strains were inoculated overnight in 5 ml of 3C media. The grown cultures were reinoculated in 5 ml of 3C in quintuplicates with a starting OD600 of 0.1. After 48 hours, when fatty acids are already present in the extracellular medium, cells were harvested by centrifugation and lysed using liquid nitrogen. Then, RNA was extracted using the RNeasy Midi kit (Qiagen) following the recommended protocol. Sequencing libraries were constructed with the Quantseq 3' mRNA library FWD kit  (Lexogen) according the manufacturer's protocols.