GSM8162163: FHD,knock-in FTOrs9939609-A, Day35; Homo sapiens; RNA-Seq
Total RNA was purified with TRIzol reagents (Thermo Scientific).RNA concentration was measured by NanoDrop 8000 Spectrophotometer (Thermo Scientific) or a Qubit Fluorometer (Thermo Scientific). A total amount of 1 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.