GSM7519967: B220PBS_1_Rloop; Mus musculus; OTHER
All the cells were first fixed by methanol, mobilized on Concanavalin A beads, folllowed by S9.6 anbibody incubation and nanobody Tn5 binding. Mg2+ was added to activate the cutting of Tn5 Recovered chromotain DNA was first end repaired using Bst 2.0, followed by in vitro transcription of T7 promoter. Transcribed RNA was first reverse converted to first cDNA and second DNA strand. Double strand DNA was cut by second Tn5. Library was amplified by DNA polymerase.