GSM8120538: control, RV, replicate3; Mus musculus; RNA-Seq
Embryonic hearts were dissected from pregnant dams at E13.5 in 1% FBS in PBS. Yolk sacs were used for genotyping. Hearts were then microdissected to obtain the interventricular septum (IVS), left ventricular (LV) and right ventricular (RV) tissue. Tissues were singularized with TryplE at 37C, quenched with 1% FBS in PBS, filtered and centrifuged at 300g for 5 minutes. The resulting pellet was resuspended in 1% FBS in PBS and counted using an automated cell counter. A 30μL aliquot of the cell suspension was used for library generation. Single cell droplet libraries were generated with the Chromium Next GEM Single Cell 5' Library & Gel Bead Kit v1.1, accoring to manufacturer's instructions.