GSM7242796: RGFP.ONC.Retina.Female.A; Mus musculus; RNA-Seq
Retinas were homogenized in RLT buffer and then total RNA extracted using a Qiagen RNeasy Mini Kit according to manufacturer's instructions. Libraries were prepared following protocols from NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra RNA Library Prep Kit for Illumina. Samples were enriched for mRNA using oligo(dT) beads, which were fragmented randomly and used for cDNA generation and subsequent second-strand synthesis using a custom second-strand synthesis buffer (Illumina), dNTPs, RNase H and DNA polymerase I. cDNA libraries then went through terminal repair, A-base ligation, adapter ligation, size selection, and PCR enrichment.