GSM6578994: ecd1, nub>Dam control; Drosophila melanogaster; ChIP-Seq
DNA extraction from dissected third instar Drosophila larvae and sequencing of methylation-specific PCR amplicons was performed according to the Nature protocols on DamID-Seq published by Vogel et al. (2007) and Marshall et al. (2016). Sequencing libraries were generated according to the Illumina protocol for ChIP-Seq library preparation. Samples were pair-end sequenced on an Illumina HiSeq 2000 instrument at 50 bp read length.