GSM7777205: BMDM cRel-/- 2.0h R2; Mus musculus; RNA-Seq
Cellular fractionation was used to isolate nuclei and extract chromatin. Once chromatin was isolated, it was resuspended in Trizol. ~100ng of chromatin-assosiated RNA was used for library construciton. RNA-seq libraries were constructed using the Illumina Stranded Tru-Seq Kit adapted from the manufacturers instructions.