GSM7777172: cRel_1.0h_R1; Mus musculus; ChIP-Seq
Cellular fractionation was used to isolate nuclei. To capture NF-kB specificity, 1.0mM DSG and 1.0% Formaldehyde were used for crosslinking.  Chromatin shearing was done with either the Bioruptor or Covaris M220. Immunoprecipitation was performed with either anti-RelA, anti-cRel, or anti-p50 antibodies. Libraries were constructed using the Kapa LTP Library Preparation Kit, according to manufacturers instructions.