GSM8331607: Healthy control, scRNA-Seq; Homo sapiens; RNA-Seq
For single-cell sequencing, peripheral venous blood (2 ml) from the severe HFMD patient and the control was collected into heparin anticoagulant tubes. PBMCs were then isolated via Ficoll density gradient centrifugation (Lymphocyte Separation Medium, Applygen, Beijing). Cell count and viability were assessed using an automated cell counter to ensure that the single-cell suspension was well dispersed, had high cell viability, and had an accurate count. We immediately subjected freshly extracted PBMC to 10× single-cell sequencing The entire process includes single-cell counting, single-cell GEM production, reverse transcription, cDNA amplification, library construction, library quality testing, and superior sequencing. The cDNA amplification products were sequentially fragmented, end completed, A-tailed, fragment size sorted, and ligated Head, and the complete library sequencing adaptor was added by PCR to obtain the sequencing library.Concentration required for library quality testing >10ng/ul, and the fragment length is concentrated between 350-500bp.