GSM7623663: 10 cGy IR, 3 hr, replicate 3; Arabidopsis thaliana; RNA-Seq
Total RNA was extracted from approximately 0.1 g whole rosette tissues using the RNeasy Plant Mini Kit (Qiagen). RNA samples were quantified using a NanoDrop spectrophotometer (NanoDrop Technologies) and quality was assessed using an Agilent 2100 Bioanalyzer. RNA-seq library construction was performed by the University of Florida Interdisciplinary Center for Biotechnology Research (UF-ICBR, RRID:SCR_019152), using poly(A) selection and the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs).