NextSeq 2000 paired end sequencing; RNA seq for PacR cells (Non-targeting vs siJunD)
Cells were reverse transfected with Non-targeting 5 or siJunD for 24 hours before harvesting. RNA was extracted using miRNeasy kit (Qiagen) following manufacturer's instructions. Libraries for cells were made using the TruSeq RNA Library Prep Kit V2 (Illumina) according to the manufacturer's instructions.