GSM7520026: A_0327p19; Mus musculus; ChIP-Seq
Cells were fixed with 1% methanol-free PFA, then sheared with micrococcal nuclease for 20 mins. Nuclei were disrupted using the diagenode Bioruptor. 7.5% of human chromatin was added to the mouse chromatin as a spike in control Library preparation was performed with the NEBNext Ultra II DNA library kit according to the manufacturer's protocol. Minimum input DNA was 4 ng per sample.