GSM6965227: VE.3296.EXP.22.BY8608.WT1; Mus musculus; RNA-Seq
Total RNA was extracted with the RNeasy Micro Kit (Qiagen, #74004) and stored at -80 C until submission. Sequencing libraries were prepared from 500ng total RNA using the TruSeq stranded mRNA library preparation kit (cat# 20020595, Illumina Inc.) including polyA selection. Unique dual indexes (cat# 20022371, Illumina Inc.) were used. The library preparation was performed according to the manufacturers' protocol. The quality of the libraries was evaluated using the Fragment Analyzer from Advanced Analytical Technologies Inc., using the DNF-910 dsDNA kit. The adapter-ligated fragments were quantified by qPCR using the Library quantification kit for Illumina (KAPA Biosystems) on a CFX384 Touch instrument (Bio-Rad) prior to cluster generation and sequencing.