GSM8291816: MB 2-7 B; Mus musculus; RNA-Seq
RNA was isolated from C2C12 cells using TRIzol reagent (Invitrogen) followed by phenol-chloroform extraction, precipitated with 100% isopropanol, and washed with 70% ethanol. RNA pellets were air-dried and resuspended in RNase-free water. RNA samples corresponding to myoblasts and myotubes (day 3 of differentiation) were submitted to the Boston University Microarray and Sequencing Resource Core in triplicate for library prep and Illumina sequencing.