DNBSEQ-G400 paired end sequencing of SAMD00631172
Genomic DNA was extracted using the Wizard Genomic DNA Purification Kit (Promega). Shearing of DNA, library preparation, and sequencing were then performed by the Bioengineering Lab Co. (Sagamihara, Japan). Extracted DNA was sheared to approximately 500 bp fragment size using a Focused-ultrasonicator (Covaris). Library preparation was performed on sheared DNA using the MGIEasy PCR-Free DNA Library Prep Set (MGI Tech) and the MGIEasy Circularization Kit (MGI Tech). The library was sequenced using a DNBSEQ-G400 system (MGI Tech) to generate 2x200 bp paired-end reads.