GSM8255128: Scolex reads 2; Taenia solium; RNA-Seq
Total RNA from each sample was extracted using Qiagen RNeasy Plus Universal mini kit (Qiagen, Hiden, Germany) and treated with TURBO DNase (Thermo Fisher Scientific, Waltham, MA, USA). RNA samples were quantified using Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA) and the integrity was checked using Agilent TapeStation 4200 (Agilent Technologies, Palo Alto, CA, USA The samples were prepared for rRNA depletion using the QIAseq FastSelect rRNA HMR Kit (Qiagen, Hilden, Germany). RNA sequencing library preparation employs the NEBNext Ultra II RNA Library Preparation Kit for Illumina and the manufacturer's recommendations were followed (NEB, Ipswich, MA, USA). Briefly, enriched RNAs were fragmented for 15 min. at 94°C. First strand and second strand cDNA were subsequently synthesized. cDNA fragments were then end repaired and the 3' ends adenylated, and universal adapters ligated to cDNA fragments, followed by index addition and library enrichment with limited cycle PCR. Sequencing libraries were validated using the Agilent Tapestation 4200 (Agilent Technologies, Palo Alto, CA, USA), and quantified using Qubit 2.0 Fluorometer (ThermoFisher Scientific, Waltham, MA, USA) as well as by quantitative PCR (KAPA Biosystems, Wilmington, MA, USA).