GSM7634534: siNS_eth_replicate2; Homo sapiens; RNA-Seq
Total RNA was extracted using Trizol (Thermo scientific) and 3 replicates for each condition were performed. cDNA libraries were prepared using the RNA-seq library prep kits with UDIs (Lexogen, Vienna Austria). Quality of the cDNA was assessed and RT-qPCR was performed for selected GR target genes as quality control. Standard illumina protocols were used to construct the library for sequencing.