GSM7159218: RCC243, DMSO, rep1; Homo sapiens; RNA-Seq
Cell pellets collected and washed in PBS. RNA isolation was performed using the Qiashredder kit (Qiagen, #79654) and the RNeasy mini kit (Qiagen, #74104). RNA quality was assessed on an RNA 6000 Pico chip (Agilent Technologies) using the Agilent Bioanalyzer to determine sample RNA integrity number (RIN) and quantified by the Qubit RNA HS assay kit (Life Technologies) MS023: NEB stranded mRNA libraries were constructed and paired-end (100bp) next generation sequencing was performed on a Illumina NovaSeq 6000 S4 system. PRMT1 KnockDown: NEB stranded mRNA libraries were constructed and paired-end (150bp) next generation sequencing was performed on an Illumina NovaSeq X system.