GSM8208257: Prkcz2, P30; Mus musculus; RNA-Seq
For total mRNA isolation, mice were euthanized at the indicated postnatal timepoints by CO2 inhalation and brains were immediately dissected on ice. Equal amounts of tissue were obtained from cerebral cortices using a 4mm punch biopsy tool, flash frozen in liquid nitrogen and stored at -80°C until further processing. Total mRNA was extracted using RNAeasy Mini Kit, as per manufacturer's instructions (QIAGEN). Library preparation and sequencing of RNA from cerebral cortex samples (3-4 biological replicates for each genotype) was performed at the Yale Center for Genome Analysis using 100-base pair, paired-end reading on a NovaSeq instrument (Illumina) at a sequencing depth of 25 million reads per sample