GSM7744015: F4/80 Brain Cells, WNV B6J 2; Mus musculus; RNA-Seq
After MACs isolation, brain macrophages were washed in PBS and cell pellets prepared using the RNAqueous Micro kit (Ambion) per manufacturer's instruction. Total RNA was concentrated by evaporation using an Eppendorf Vacufuge system and was then added to lysis buffer from the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara).  Reverse transcription was performed followed by 20 cycles of PCR amplification to generate full length amplified cDNA.  Sequencing libraries were constructed using the NexteraXT DNA sample preparation kit with unique dual indexes (Illumina) to generate Illumina-compatible barcoded libraries.