GSM6859057: s_EMT6_lucV5_BrM2_3; Mus musculus; RNA-Seq
RNA was extracted from lysates using Rneasy Mini columns (Qiagen). After RiboGreen quantification and quality control by Agilent BioAnalyzer, 500ng of total RNA underwent polyA selection and TruSeq library preparation according to instructions provided by Illumina (TruSeq Stranded mRNA LT Kit, #RS-122-2102), with 8 cycles of PCR.