GSM6965166: sams5_1; Caenorhabditis elegans; RNA-Seq
Young adult C. elegans were lysed in 0.5% SDS, 5% b-ME, 10 mM EDTA, 10 mM Tris-HCl pH 7.4, 0.5 mg/ml Proteinase K, then RNA was purified with Tri-Reagent (Sigma) RNA libraries were prepared for sequencing using standard Illumina protocols