GSM8427626: shCLIC1 rep1; Homo sapiens; RNA-Seq
Total RNA was extracted using RNAiso Plus reagent (Takara Bio, #9109) following the manufacturer's protocol. Then, RNA quality was determined by a 5300 Bioanalyzer (Agilent) and quantified using an ND-2000 (NanoDrop Technologies). Only high-quality RNA samples (OD260/280=1.8~2.2, OD260/230≥2.0, RIN≥6.5, 28S:18S≥1.0, >1 μg) were used to construct the sequencing library. RNA libraries were prepared for sequencing using standard Illumina protocols