GSM8119445: FL_3; Gallus; Hi-C
Cells were treated with formaldehyde to fix the conformation of DNA. After cell lysis, cross-linked DNA was digested with restriction enzymes to generate sticky ends. Biotinylated DNA was captured using streptavidin magnetic beads. The DNA fragments were then end-repaired, adenylated, and ligated to adapters. The optimal number of PCR amplification cycles was evaluated before purifying the library, completing the library preparation.