GSM8072102: P60_WM_scATAC-seq; Mus musculus; ATAC-seq
For single-cell isolation of the early postnatal (P7) and adult (P60 and P90) cortex, thick coronal sections, spanning the rostral end of the lateral ventricles to the rostral end of the hippocampus, were generated from each brain. For whole cortex datasets (P7 cortex scATAC-seq data) the region dorsal to the lateral ventricles was dissected out. This region contained the ventricular-subventricular zone (V-SVZ), cortical white matter, and cortical grey matter. For grey matter datasets (P60 grey matter scATAC-seq data), coronal sections were taken as described above and the region dorsal to the cortical white matter was dissected out. For white matter datasets (P60 white matter scATAC-seq data, P90 control scATAC-seq data, P90 cuprizone-rapamycin scATAC-seq data), coronal sections were taken as described above and the region immediately dorsal to the lateral ventricles, containing both the corpus callosum and V-SVZ, was dissected out. Libraries were prepared according to the manufacturer's instructions (10X Genomics) using the Chromium Single Cell Next GEM Single Cell ATAC Library and Gel Bead Kits.