GSM7158623: TotalRNA_K562_cMYC_3UTRmut_REP2_ACTD_6hr; Homo sapiens; RNA-Seq
K562 HDR-edited cells were centrifuged (500g at 4°C for 4 minutes), washed in ice-cold PBS and resuspended in 350μl of Buffer RLT Plus supplemented with 1% (v/v) β-mercaptoethanol from the RNeasy Plus Mini Kit. RNA was extracted using the same kit according to manufacturer's instructions and quality was assessed using the Agilent 2200 TapeStation System  with RNA ScreenTape and Sample Buffer. S2 spike-in material (5%) was added to RNA. NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with ribodepletion using the NEBNext rRNA Depletion Kit. Total RNA-seq