GSM8120422: Colon, WT W6, replicate 1; Mus musculus; RNA-Seq
Tissue biopsies (approximately 0.5 cm long) were collected from the distal colon and stored at -80°C in RNAlater (Invitrogen). To extract RNA, tissue was lysed in RLT Plus Buffer (QIAGEN) + 2% b-mercaptoethanol by bead-beating (Precellys). RNA was isolated with the RNeasy Plus Mini Kit (QIAGEN) according to the manufacturer's instructions and quantified using a Nanodrop spectrophotometer (ThermoFisher). cDNA libraries were sequenced by Novogene Co. Ltd (UK) at a sequencing depth of 20 million reads per sample using paired-end 150 bp reads on a NovaSeq 6000 sequencer with a v1.0 reagent kit. Samples were sequenced using paired-end 150bp sequencing, aiming at a coverage of 20M reads.