GSM8072070: esophageal epithelial cells, lane1; Mus musculus; RNA-Seq
The esophageal muscle layer and submucosa were removed mechanically and after enzymatic treatment. The epithelium was minced and incubated in DNaseI and LiberaseTM. After incubation, the cells were filtered and stained with CD49f-PE and CD104-A647. Using FACS, double-positive cells (CD49f+CD104+) were isolated. RNA isolation and library preparation of the sorted CD104/CD49f double positive epithelial cell solution for the 10X Chromium platform were conducted by NGI-Sweden (National Genomic Infrastructure) at SciLifeLab, Stockholm.