GSM8292094: Intestinal lamina propria cells from nulliparous mice; Mus musculus; RNA-Seq
Using FACSAria III Cell Sorter (BD), CD45-positive and EPCAM-negative cells (Immune cells) and  CD45-negative and EPCAM-negative cells (Mesenchymal cells) were enriched by cell sorting from nulliparous and lactating mice. For each sample, one million cells were fixed for 22 hours at 4°C, quenched and stored at -80°C according to 10X genomic Fixation of Cells & Nuclei for Chromium Fixed RNA profiling (CG000478, 10X Genomics, Pleasanton, CA) using the Chromium Next GEM Single Cell Fixed RNA Sample preparation kit (PN-1000414, 10X Genomics). 250,000 cells per sample were used for probe hybridization using the Chromium Fixed RNA Kit, Mouse WTA probes (PN-1000496, 10X Genomics), pooled at equal numbers and washed following the Pooled Wash Workflow following the Chromium Fixed RNA Profiling Reagent kit protocol (CG000527, 10X Genomics). GEMs were generated using Next GEM ChipQ (PN-1000422, 10X Genomics) on Chromium X (10X Genomics) with a target of 10,000 cells recovered Libraries prepared according to the manufacturer instructions (CG000527, 10X Genomics). Sequencing was performed using NovaSeq S4 lane PE150 (Illumina) with a target of 15,000 reads per cell. Alignment of the samples was performed with the 10x Genomics Cell Ranger 7.1.0 multi pipeline.