GSM7039145: CL08_KPV_VIM_KO_1; Mus musculus; RNA-Seq
Cells were cultured for one passage, then lysed using RLT lysis buffer (Qiagen), and total RNA was isolated with the RNeasy Plus Mini Kit (Qiagen). RNA quality was confirmed with a TapeStation 4200 (Agilent); all samples had an RNA integrity number (RIN) score equal to or greater than 9.8. Next, mRNA was isolated via poly(A) enrichment (NEBNext). Libraries were prepared using NEBNext RNA Ultra chemistry (New England Biolabs).