GSM7623337: ChAR-Seq_P400_degron_0h_rep2; Mus musculus; OTHER
Extracted twice using Trizol reagent following the instruction The chromatin RNAs were isolated and examined, the pretreated cells without crosslink were used to chromatin isolation as described above, then the native chromatin were extracted twice using Trizol reagent to obtain the chromatin associated RNA. After checked the quantity and quality and poly(A) RNA depletion, the ChAR-seq RNA library were generated using the no coding RNA library method with random primers and sequencing analyzed using Nova PE150 platform. ChAR-Seq