GSM7962172: Naïve, PreCAR, Exp1, TR3 [RNA-seq]; Mus musculus; RNA-Seq
For RNAseq, cells were homogenized in QIAzol Lysis Reagent and processed using the miRNeasy Micro Kit with on-column DNase treatment, both according to manufacturer protocols. RNA purity, quantity and integrity was determined with NanoDrop and TapeStation 4200 analysis. The Universal Plus mRNA-Seq library preparation kit with NuQuant was used with an input of 200ng of total RNA to generate RNA-Seq libraries.