GSM7776375: Dehydrated Female 03; Jaculus jaculus; RNA-Seq
Fresh frozen brains were sliced on the coronal plane at 100microns on a cryostat. Twelve bilateral punches of the SON were taken using a 1mm sample core within the cryostat chamber kept at -20C and kept on dry ice. A pestle and mortar with liquid nitrogen was used to break up the sample. Powdered tissue was lysed using no more than 100ml/mg of Trizol. Chloroform phase separation was used to isolate RNA then cleaned up using Zymo Direct-Zol RNA miniprep kit as per manufacturer's instructions. All storage was at -80C.