GSM8162866: NP_Week6_Rep1; Rattus norvegicus; RNA-Seq
For the bone marrow, tissue dissociation was achieved with red blood cell lysis buffer, followed by enzymatic digestion using Type IV collagenase, dispase, and DNase. Bone tissue was mechanically disrupted into fine pieces and further digested with Type I collagenase. Cells released from the marrow and bone were pooled and submitted to the GRC for mRNA extraction. mRNA libraries for RNA-Seq were prepared using the Illumina mRNA prep kit following manufacturers protocols