GSM7110544: Donor 1216, Minimal residual disease; Homo sapiens; RNA-Seq
Bone marrow aspirates were obtained from The Finnish Haematology Registry and Clinical Biobank. The mononuclear cell fraction was isolated using Ficoll density gradient separation. Cell counting and viability assessments were conducted using To-Pro-3 viability dye. Thereafter, GEM generation & barcoding, reverse transcription, cDNA amplification and 3' gene expression library generation steps were all performed according to the Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (Single Index) User Guide (10x Genomics CG000204 Rev D) with all stipulated 10x Genomics reagents.