GSM7442055: Eif4enif1+/-_2_transcription; Mus musculus; RNA-Seq
For T&T seq transcriptome and RNA-seq data,mRNA was extracted by lysing oocytes or embryos in lysis buffer for direct reverse transcription  Vazyme Single Cell Full Length mRNA-Amplification Kit. For T&T-seq translatome data, ribosome-bound mRNAs were extracted using RiboLace Module 1 Kit following manufacturer's  guidelines and purified using VAHT RNA clean beads. The purified mRNAs were then reverse transcribed with Vazyme Single Cell Full Length mRNA-Amplification Kit. cDNAs were purified using VAHT DNA clean beads. DNA libraries for RNA-seq were prepared using TruePrep DNA Library Prep Kit V2 for Illumina following manufacturer's instructions.