GSM6422193: X1751756_VilCreerZNKP_small_intestine_mass_het_2; Mus musculus; RNA-Seq
Total RNA was extracted from tissues using Maxwell RSC miRNA Tissue Kit (AS1460, Promega). RNA quality was assessed using TapeStation System (5067-5579, Agilent). The Illumina TruSeq stranded mRNA kit (20020595, Illumina) was used to prepare RNA libraries and RNA quality confirmed using Tapestation (Agilent) and quantified using Kapa qPCR library quantification kit for Illumina platforms (KK4873,Kapa Biosystems). Samples were normalised using the Agilent Bravo, pooled and sequenced on NovaSeq flow cell to generate single end 50bp reads at 20M reads per sample.