GSM7864924: ATAC-seq of control SCD fed mice without STZ treatment, 20w, biological replication 1; Mus musculus; ATAC-seq
ATAC-seq libraries were prepared from 5 mg of fresh liver tissue. To prepare ATAC-seq library, nuclei were extracted from liver cells using the Singulator 100 system (S2 Genomics, CA, USA) with an extended nuclear dissociation protocol. 50,000 nuclei were transferred to an EP tube containing 1 mL of ATAC-seq resuspension buffer, then transposition solution was adde, and eluted DNA was purified and amplifired with Nextera DNA Flex kit(Illumina).