GSM7864396: Retinosphere Supplement Reprogramming ASCL1 scRNA; Homo sapiens; RNA-Seq
Tissue was dissociated with papaine and DNase (Worthington),. Cultured cells were dissociated with Accutase (Sigma). All dissociated cells spun at 300 g (4 C), then resuspended in culture medium to reach 1000 cells/uL. 10x Chromium Single Cell 3' V3.1 (scRNA-seq) and 10x Chromonium Next Gem Single Cell Multiome ATAC + Gene Expression (Multiome)