GSM6755272: Fig4 double negative, P4, replicate 2 [E2_Fig4nn_P4_2]; Mus musculus; RNA-Seq
Total RNA was isolated using Qiagen RNAeasy Plus Micro kit (# 74034). The Agilent Bioanalyzer (Tape Station) was used to determine RNA integrity (RIN) prior to library preparation. Stranded mRNA sequencing libraries were prepared using the TruSeq Stranded mRNA Library Prep Kit according to manufacturer's instructions (Illumina).