GSM8119613: Emue_MicroC; Ephydatia muelleri; OTHER
Capsaspora owczarzaki cells were double crosslinked with 1% formaldehyde and 3 mM DSG (Thermo Scientific, A35392).  Mnemiopsis leidyi specimens were dissociated into single cells in Ca2+/Mg2+-free artificial sea water by triturating every 2 min for a total of 10 min. Dissociated cells were filtered through a 40 µm cell strainer and double crosslinked with 1% formaldehyde and 3 mM DSG. Seven days post hatching sponges Ephydatia muelleri were fed with 0.5 µm fluorescent carboxylate-modified FluoSpheres (Invitrogen, F8813). Next, animal tissues were dissociated into cells and double crosslinked with 1% formaldehyde and 3 mM DSG. Crosslinked cells were filtered through 40 µm cell strainer, stained with 2 µg/ml Hoechst 33342, and FACS sorted to isolate choanocytes. Trichoplax adhaerens specimens were dissociated into single cells in Ca2+/Mg2+-free artificial sea water. Dissociated cells were filtered through a 40 µm cell strainer and double crosslinked with 1% formaldehyde and 3 mM DSG. Micro-C libraries were prepared as previously described by Hsieh et al., 2020 (PMID: 32213323) and Krietenstein et al. 2020 (PMID: 32213324).