GSM8291401: Plasma, treatment, 31days post-stroke, biol rep 1, microRNA; Rattus norvegicus; miRNA-Seq
The serum EVs were isolated using size exclusion chromatography techniques as described previously by Boing et al. (doi: 10.3402/jev.v3.23430) The isolation of EVs from brain tissue was performed using a modified protocol originally described by Vella et al. (doi: 10.1080/20013078.2017.1348885) The total RNA was extracted and purified from EVs using the miRNeasy Advanced Kit (Qiagen, cat. No. 217204) according to the kit instructions. The RNA concentration and purity were then evaluated using the RNA Nano 6000 Assay Kit on the Agilent Bioanalyzer 2100 System (Agilent Technologies, CA, USA). For small RNA library preparation, 1ng-500ng RNA per sample was used as input material for libraries created with the QIAseq miRNA Library Kit (Qiagen, Frederick, MD) For long RNA library preparation, 250pg-10ng RNA per sample was used as input material for libraries created with the SMARTer Stranded Total RNA-Seq Kit (Takara Bio USA, Inc.)