GSM8257778: M07e_JAK3Q988P_untreated_biol rep 3; Homo sapiens; RNA-Seq
Total RNA was isolated using the miRNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. RNA quantification and quality were checked by Nanodrop (Thermo Fisher Scientific Inc.), Qubit (Thermo Fisher Scientific Inc) and TapeStation (Agilent Technologies). Using the TruSeq Stranded Total RNA Library Prep (Illumina, Inc.) and included rRNA depletion, fragmentation, cDNA synthesis and adaptor ligation. The generated libraries were normalized and combined in equimolecular concentrations for optimal generation of DNA clusters.