GSM7776176: immune cell, rep2, scRNAseq; Mus musculus; RNA-Seq
Colons were digested and single cell suspensions obtained using 70-μm filters (BD, Franklin Lake, NJ, USA). After incubation with red blood cell lysis buffer, CD45+ immune cells were isolated by positive selection with anti-mouse CD45 magnetic beads (Miltenyi, Germany), according to the manufacturer's instructions. Finally, cells were resuspended in complete RPMI-1640 medium, counted for viability, and then immediately subjected to library preparation. ScRNA-Seq libraries were prepared using 10x Single Cell Genomics technology (10x Genomics, San Diego, CA, USA), according to the manufacturer's instructions.