GSM8291345: callus, Drought; Arabidopsis thaliana; miRNA-Seq
Callus tissues were collected after 7 days in the growth chamber. Samples were stored at -80°C for further analysis Total RNA was isolated from Arabidopsis thaliana callus tissues using Hibrizol (HibriGen, Turkey) and phenol:chloroform Ligation of 3' and 5' adapters to the 3' and 5' ends of the small RNA and cDNA synthesis Purification and size selection of libraries containing 18-40 bp Illumina NextSeq 2000 Single End Small RNA-Seq