GSM8331740: Tet IL23, PB61; Homo sapiens; ATAC-seq
Nuclei from 90000 sorted MAIT cells were isolated using ATAC-lysis buffer from the Active Motif ATAC-seq Kit following manufacturer's instructions. Libraries were prepared using the ATAC-seq kit from Active motif according to manufacturer's instructions. After centrifugation (2800 rpm, 10 min 4°C), nuclei were incubated with the tagmentation master mix in a shaking heat block for 30min at 37°C with agitation at 800rpm. DNA was then purified and amplified for 10 cycles using indexed primers, and size-selected using SPRI bead solution.