GSM7233426: R1-AD1 FLAGHA-H3F3A cells, R1881, 12 h, AR Ab, rep1; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde in PBS for 10 min at RT, quenched with glycine (f.c. 0.125 M) for 5 min. After cell lysis chromatin was sheared by Diagenode Bioraptor Pico with peak enrichment at 200 bp. ChIP-seq libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs)