GSM8330462: stomach, normal, BRG1 ChIP, rep1; Mus musculus; ChIP-Seq
Isolated gastric glands were fixed in 1% PFA for 15min and quenched with glycine. For ARID1A and BRG1, an additional crosslinking step with 2mM DSG for 45min were performed prior to PFA fixation. Cells were lyzed and sonicated to obtain chromatin for IP. ChIP-seq libraries were prepared according to the standard Illumina protocols. Briefly, DNA was end-repaired and ligated to sequencing adaptors, then PCR amplified and gel purified. The purified DNA libraries were sequenced following the manufacturer's protocols.