GSM8119628: CPN_02; Mus musculus; ATAC-seq
Viable FACS sorted cells, 50,000 per biological replicate, were lysed in non-ionic detergent to isolate the nuclei. The nuclei were suspended in a transposition mix, and incubated at 37oC to generate DNA fragments. The DNA fragments were amplified using indexing primers. The product was purified once more to give the final library. Library concentrations were in the nano-molar range. The library quality was assessed using a BioAnalyzer. Samples with an RNA integrity number above 9 were used for ATAC-seq library sequencing.