GSM8257875: DB05001; Homo sapiens; RNA-Seq
Total RNA was isolated from approximately 25 × 106 PBMCs using Trizol® Reagent (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions. Total RNA was quantified using a NanoDrop-1000 spectrophotometer (Peglab, Erlangen, Germany), and RNA quality was monitored by an Agilent 2100 Bioanalyzer (Agilent, Böblingen, Germany). Sequencing libraries were prepared at the Core Facility Genomics, Medical University of Vienna using the NEBNext Poly(A) mRNA Magnetic Isolation Module and the NEBNext UltraTM II Directional RNA Library Prep Kit for Illumina according to the manufacturer's protocols (New England Biolabs, Ipswich, MA) with unique dual indices (UDI).