GSM8426977: Week4_surface; Homo sapiens; RNA-Seq
GBM cells were dissociated with TrypLE (Thermo Fisher) and incubated with TotalSeq-B anti-human antibodies (BioLegend) according to manufacturer instructions. TotalSeq-B0253 and B0254 were used for the two replicates. scRNAseq libraries were generated using the Chromium Next GEM Single Cell GEM, Library & Gel Bead Kit v3.1, Chromium Single Cell Feature Barcode Library Kit, Chromium Next GEM Chip G, and 10x Chromium Controller (10x Genomics) according to manufacturer instructions. The Cas9-edited Molecular Recorder barcodes were PCR amplified from single-cell cDNA libraries as previously described [Chan et al., 2019].