NextSeq 2000 paired end sequencing; H3K4me3 HiChIP for Paclitaxel resistant L3.6pl pancreatic cancer  cells (Non targeting vs siJunD)
Cells (24 hours of knockdown) were washed with PBS and trypsinized. Cells were fixed for 10 minutes with 1% Formaldehyde and suspended in ice-cold Hi-C Lysis Buffer (10mM Tris-HCL pH 7.5, 10mM NaCl, 0.2% NP-40 in water). Streptavidin-C1 beads (Thermo Fisher) were used to capture biotinylated DNA Library preparation using the MicroPlex Library Preparation Kit (Diagenode)