GSM6716961: UM-UC-3 cisplatin resistant 1; Homo sapiens; RNA-Seq
Total RNAs were isolated by an Rneasy kit (Qiagen). The cDNAs were synthesized from total RNA using random primers. The ribose diols in the 5'-cap structures of RNAs were oxidized and then biotinylated. The biotinylated RNA/cDNAs were selected by streptavidin beads (cap-trapping). After RNA digestion by RNaseONE/H and adaptor ligation to both ends of cDNAs, double-stranded cDNA libraries (CAGE libraries) were constructed.