GSM8257419: splenic WT DC, scRNA-seq; Mus musculus; RNA-Seq
Splenic DC were enriched from splenocyte suspensions using EasySep Mouse CD11c Positive Selection Kit II (Stemcell) according to the manufacturer's instructions. Then, about 1.5 × 10^4 live CD45.2+ CD11c+ cells were FACS sorted from WT or H2-Aa cit/cit DC enriched samples. scRNA-seq libraries were generated using the Chromium Next GEM Single Cell 3' kit (10X Genomics) according to the manufacturer's protocol. Purified libraries were sequenced on one S4 lane of a NovaSeq sequencing instrument