GSM8033122: HeLa, GFP-NLS-TAF2dIDR expression, TAF7, CUT&RUN; Homo sapiens; ChIP-Seq
Standard CUT&RUN according to Skene et al., 2018 NEB Next Ultra II library preparation kit (NEB #E7645L) without size selection according to manufacturer's instructions with modifications: we increased the end prep duration to 60 min at 50 °C instead of 30 min at 65 °C and used only 10 s annealing and extension time instead of 75 s, and 15 PCR cycles during the amplification step