GSM6965734: dBFD1_2; Toxoplasma gondii; RNA-Seq
To harvest, infected neurons were dissociated by treatment with accutase enzyme for 5 mins at 37°C, then pelleted by centrifugation and flash frozen for storage at -80°C. Total cellular RNA was isolated using the Zymo Direct-zol RNA Miniprep Kit and RNA quality was assessed using a BioAnalyzer or Fragment analyzer. Libraries for RNA-seq were prepared using the Swift Low-Input Library Prep Kit with poly(A) enrichment following manufacturer's protocols.