GSM8255987: Hct116 cells, parental, rep3; Homo sapiens; RNA-Seq
Qiagen RNeasy Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. Final steps included end repair, A-tailing, adapter ligation, size selection, amplification, and purification.