GSM7776986: KPPMPIK_replicate_2; Mus musculus; RNA-Seq
RNA was extracted using the Rneasy Plus Kit (Qiagen) according to the manufacturer's instructions. RNA quality and quantification were carried out using a Nanodrop spectrophotometer (Thermo Fisher) or a Bioanalyzer (Agilent). Total RNA (500 ng) was used for reverse transcription using TaqMan Reverse Transcription Reagents (Thermo Fisher), and real-time PCR was performed using the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad; RRID:SCR_018064). Microdissected tissue was enzymatically digested into a single-cell suspension using the Papain Dissociation System according to the manufacturer's instructions (Worthington Biochemicals). The samples were sequenced using the 10X Chromium single-cell 3' protocol.