GSM8120370: mRNA_ded1-ΔN_2; Saccharomyces cerevisiae; RNA-Seq
For RNA-seq library preparation, total RNA was extracted and purified from aliquots of the same snapped-frozen cells described above using hot phenol-chloroform extraction. Libraries for mRNA-Seq were prepared by the NHLBI DNA Sequencing and Genomics Core at NIH (Bethesda, MD). Illumina TruSeq stranded mRNA kits were utilized, followed by sequencing with SR100 to prepare libraries from total RNA.