GSM6422274: PTEN KD Regenerating RGCs; Mus musculus; RNA-Seq
Custom designed Drop-Seq barcodes from Integrated DNA Technologies (IDT) were delivered into wells of two 384-well plates. All primers in one well shared the same unique cell barcode and billions of different unique molecular identifiers (UMIs). Following cDNA synthesis, the contents of each well were collected and pooled into one tube using a Caliper SciClone Liquid Handler. After treatment with exonuclease to remove unextended primers, the cDNA was PCR amplified for 13 cycles and the cDNA was fragmented and amplified for sequencing using a Nextera XT DNA sample prep kit (Illumina).