GSM7886462: HPAD_11_EPZ; Homo sapiens; RNA-Seq
Treated human ex vivo pancreatic cells and human pancreatic ductal epithelial cells were lysed using TRIzol reagent (Invitrogen), and Total RNA was extracted from the cells using RNeasy Kit (QIAGEN) including DNase treatment according to manufacturer protocol Barcoded RNA-seq libraries were generated from ribosomal RNA depleted total RNA using the NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) (New England Biolabs, Ipswich, MA) in conjunction with the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (New England Biolabs) using 1μg of input total RNA and following the manufacturer's instructions for depletion and library construction