GSM8427595: Organoids, Passage 1, 12-week-old mouse; Mus musculus; ATAC-seq
ATAC-seq libraries were prepared using Nextera DNA Sample Preparation Kit following the manufacturer`s protocol. Following PCR amplification, DNA was purified using the Qiagen MinElute kit and run on a 2% E-gel agarose gel. The concentration of the libraries was determined using a Qubit and 2100 Bioanalyzer. ATAC-seq libraries were prepared using Nextera DNA Sample Preparation Kit following the manufacturer`s protocol. Following PCR amplification, DNA was purified using the Qiagen MinElute kit and run on a 2% E-gel agarose gel. The concentration of the libraries was determined using a Qubit and 2100 Bioanalyzer. 1,6 pM of libraries with 5% PhiX was then loaded on a NextSeq 500 (Illumina) for a 75 bp paired-end sequencing run at the Research Sequencing Facility of ERIBA (UMCG).