GSM7626933: WTF_3m_ATAC_rep2; Mus musculus; ATAC-seq
100,000 primary cholangiocytes were lysed in cold ATAC lysis buffer (10 mM Tris–HCl pH 7.5, 10 mM NaCl, 3 mM MgCl2, 0.1% (v/v) igepal CA-630) for 20 min on ice before centrifuged at 500 g for 10 min. The cell nuclei were collected and incubated with 50 μL transposition reaction buffer (3 μL TruePrep Tagment Enzyme, 10 μL TruePrep Tagment Buffer L from Vazyme TD501) at 37°C for 60 min. DNA was purified using the QIAquick PCR Purification Kit (QIAGEN, 28106) according to the manufacturer's instructions. After DNA fragmentation and purification, library construction was performed with TruePrepTM DNA Library Prep Kit V2 for Illumina (Vazyme, TD501) according to the manufacturer's instructions.