GSM8032740: HeLa, GFP-NLS-TAF2dIDR, dox, rep1; Homo sapiens; RNA-Seq
Total RNA was isolated using an RNA isolation kit (RNeasy Mini Kit, Qiagen #74106). 8 ug of RNA were treated with DNaseI and purified according to the manufacturer's protocol (TURBO DNA-free kit, Thermo Fisher Scientific #AM1907) Libraries were prepared with 100 ng RNA starting material using the Illumina Standard mRNA Prep kit (Illumina, 20040534). Oligo(dT) magnetic beads purify and capture polyadenylated RNAs. PolyA+ RNAs were fragmented and copied into first strand cDNA using reverse transcriptase and random primers. In a second strand cDNA synthesis step, dUTP replaces dTTP to achieve strand specificity. After adapter ligation, the resulting products were purified and amplified using unique dual index primers for multiplexed sequencing.