GSM8070226: Hypoxia_H3K9la_IP; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. MNase treated cells for 15 min at 37℃. The DNA were purified using glycogen. 10ng DNA was used as starting material for input and ip samples.After the DNA samples quality control，the purified DNA was used NEBNext® Ultra™ II DNA Library Prep Kit （NEB, USA, Catalog #: E7645L）for ChIP-seq library preparation.