GSM8208825: AntagomiR-141 treated cells; Oryctolagus cuniculus; RNA-Seq
Before RNA isolation, the 3T3-feeder cells were removed by a 5-min incubation with 0.02% (w/v) EDTA  in PBS. Samples were washed twice with PBS; afterwards, total RNA was isolated at 4th, 6th and 10th days in culture, and 48 hrs after transfection of antagomiR-141, with Trizol reagent according to the instructions of the manufacturer, then resuspended in DNase/RNase free water. cDNA libraries were generated using the Illumina TruSeq RNA Sample Preparation Kit according to the manufacturer's instructions.