GSM7159100: Somatosensory cortex, WGBS, Ctrl, rep1; Mus musculus; Bisulfite-Seq
Extracting DNA from tissues using the ctab method WGBS was performed and analyzed in Novogene (Beijing, China). The concentration and quality of DNA was assessed on Qubit 2.0 Flurometer (Life Technologies, CA, USA) and an Agilent Bioanalyzer 2100 system (Agilent Technologies), respectively. Briefly, the genome DNA (100 ng) was randomly broken to 200-300 bp with Covaris S220. These DNA fragments were treated with bisulfite using Scale Methyl-DNA Lib Prep Kit for Illumina. Finally, the DNA library was obtained by PCR amplification and adding adaptor.