GSM7349266: Mfn2-/-, day +8, RepF; Mus musculus; RNA-Seq
Cells were cultured and differentiated as described above, then briefly washed in PBS and lysed in RLT Buffer (Qiagen) with 1% 2-mercaptoethanol then passed through Qiashredder tubes (Qiagen). RNA was extracted using the RNeasy Isolation Kit (Qiagen) according to the manufacturers' protocol.  RNA was quantified using Agilent 2100 Bioanalyzer (Agilent Technologies Inc) and only samples with RNA Integrity Number ≥8 were used for library preparation. cDNA libraries were made using Illumina TruSeq RNA sample kits and sequencing was performed on Illumina NovaSeq 6000 with paired-end 150 bp reads (Novogene, Cambridge, UK). Raw reads all passed quality control for Qscore, error rate distribution, and AT/GC distribution.