GSM6921898: Donor1_I_24hr; Homo sapiens; RNA-Seq
Homogenized SDS lysates were diluted 1:1 with RNA lysis buffer (Agilent) and RNA was extracted using the Absolutely RNA Microprep Kit (Agilent) according to the manufacturer's protocol, including on-column DNase treatment 150 pg of cDNA was used to generate Illumina compatible sequencing libraries with the NexteraXT library preparation kit (Illumina, San Diego, CA) per manufacturer's protocols. The amplified libraries were hybridized to the Illumina flow cell and sequenced using the NovaSeq6000 sequencer (Illumina, San Diego, CA)