GSM8257747: SCI; Mus musculus; ChIP-Seq
To isolate astrocytes, single-cell suspensions of spinal cords were prepared. Astrocytes were labeled with anti-ACSA-2-conjugated MicroBeads (130-096-054, Miltenyi Biotec) on ice and then separated using a MACS multistage separator according to the manufacturer's instructions. After obtaining the DNA samples, the samples were first tested for quality. After passing the quality test, library construction was carried out, the specific steps are as follows: 1. DNA fragment end repair, add A base at the 3' end, and connect the sequencing junction. 2. PCR amplification and library fragment size selection (generally 100-300bp, including the junction sequence). 3. After the library construction is completed, the library will be tested for quality. The library that passes the quality test will be used for on-line sequencing.