GSM8208857: d28_KO1_Tcm; Mus musculus; RNA-Seq
RNA was extracted using the RNeasy® Mini Kit (Qiagen) following the instructions of the manufacturer For the preparation of NGS libraries, we followed the Smart-seq2 protocol. The subsequent library preparation from the amplified cDNA was performed using the Nextera XT DNA library prep kit (Illumina, San Diego, CA, USA). Library concentrations were quantified with the Qubit 2.0 Fluorometric Quantitation system (Life Technologies, Carlsbad, CA, USA) and the size distribution was assessed using the 2100 Bioanalyzer instrument (Agilent, Santa Clara, CA, USA). For sequencing, samples were diluted and pooled into NGS libraries in equimolar amounts.