GSM7697987: HT29_MeRIP; Homo sapiens; RIP-Seq
300µg of total RNA was extracted from the cell lines using Trizol and Nucleospin RNA extraction kit(macherey nagel-GMBH) Approximately 300µg of total RNA was initially used for the fragmentation process to achieve the fragment length of ~100 nucleotides. The fragmented RNA was further enriched using Magna MeRIP™ m6A Kit- Transcriptome-wide Profiling kit (Cat. no: 17-10499, Sigma-Aldrich, USA) according to the manufacturer's instructions to get m6A enriched RNA. About 10% of the fragmented RNA was saved as Input control.