GSM8032899: Mut, Heart, 12 wks, re3; Mus musculus; RNA-Seq
Mouse hearts were lysed using TRIzol reagent (Thermo Fisher Scientific, 15596026), and total RNA was extracted. Total RNA from perfused hearts was cleaned up with the RNeasy Mini kit (QIAGEN, Germantown, MD, USA, 74106). RNA libraries for RNA-seq was prepared  using a NEBNext Ultra II RNA Library Prep Kit for Illumina.