GSM8291519: wild-type C2C12 cells_3; Mus musculus; RNA-Seq
1ml Trizol was added to a 10cm dish and left to stand for 10min, the cell lysate was collected and then placed in liquid nitrogen for quick freezing.. Samples were sent on dry ice to Novogene Technology Co., Ltd for immediate downstream RNA sequencing. In brief, following RNA isolation with Trizol (catalog #, check with Novogene), a total amount of 2 μg RNA per sample was used as input material for library preparations. Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (#E7530L, NEB, USA) following the manufacturer's recommendations and index codes were added to attribute sequences.