GSM8070797: Endogenous_CD45cells_youngTME_scRNAseq; Mus musculus; RNA-Seq
Live tumor-infiltrating CD8+ T cells (or OT-1 CD8+ T cells) and/or intratumoral CD45+ cells were sorted from tumors on day 15 after tumor inoculation by BD FACSSymphony S6 Sorter (BD).  Sorted cells were pooled into groups (five biological replicates per group) with independent mice being labeled with TotalSeq-B Hashtags (Biolegend). Cells were counted and loaded onto the Chromium Controller (10X Genomics) for a target recovery of 10,000 single cells per run. Library was performed according to the manufacter's instructions (single cell 3' v3.1 protocol, 10x Genomics).