GSM7440011: Microglia from CD4 depletion + anti-CTLA4 treated sample 2; Mus musculus; RNA-Seq
Microglia (CD45intCX3CR1+) were sorted from the glioma bearing mice in different treatment  groups (IgG vs. αCTLA-4 vs. αCTLA-4+αCD4). Total RNA was isolated using Trizol–Qiagen  RNAeasy Micro kit and sent to Salk next generation sequencing core for ultra-low input RNA-Seq  cDNA library preparation and sequencing run on Illumina Hiseq2500. cDNA library preparation