GSM8255120: Melanoma, ffpe (GB124); Homo sapiens; miRNA-Seq
RNA was extracted using the Qiagen miRNeasy FFPE kit or Qiagen miRNeasy Serum/Plasma Advanced Kit. Extracted RNA was purified using the Amicon Ultra 0.5 Centrifugal filter columns for 90 minutes at 10,000g. Final sample concentrations were determined with the Invitrogen microRNA Qubit Assay and Bioanalyzer , to ensure an adequate quantity and quality of microRNA-enriched total-RNA necessary for next generation sequencing. Libraries were prepared from a 5ng RNA input using the PerkinElmer NEXTFLEX Small RNA-Seq Kit v4, incorporating Unique Dual Indexes (UDIs) and tRNA/YRNA blockers for plasma samples to enrich microRNA content.