GSM8160999: Round spermatids, WT, CUT_Tag, bio rep1; Caenorhabditis elegans; OTHER
300 thousand round spermatids per sample were prepared and 30 of 293-F cells were added as spike-in. Cells were washed twice using 1×PBS by gently pipetting, and centrifuged for 5min at 1700×g. Each sample was added with 100μl pre-chilled NE buffer, resuspended by gently pipetting up and down, and incubated on ice for 10 minutes. The libraries were prepared following the protocol of Hyperactive Universal CUT_Tag Assay Kit for Illumina Pro (catalog no. TD904, Vazyme) and were evaluated using an Agilent 2100 Bioanalyzer System (High Sensitivity DNA Analysis kit).