GSM7964213: Medulloblastoma, Ptf1a-Cre/+; Ptch1fl/fl; Pgbd5-/-, ATAC, rep 2; Mus musculus; ATAC-seq
Frozen pieces (~50 mg) of tumors from Ptf1a-Cre/+; Ptch1fl/fl; Pgbd5+/+ (n=3) and Pgbd5-/- (n=3) were used for nuclei isolation. Nuclei extraction was performed according to the protocol from Masilionis et al 2022 (doi:10.17504/protocols.io.j8nlkkmq6l5r/v1). Singlulator100 (S2 Genomics) was used for nuclei extraction. Extracted nuclei were stained with 7-Aminoactinomycin D (Invitrogen) and FACS-sorted for further nuclei purification. Ten thousand nuclei were targeted for library construction using ChromiumNextGEM Multiome ATAC + Gene Expression kit (10xGenomics) Other: Single cell multiome ATAC + Gene Expression