GSM6533817: liver tissue, OE(KO) I/R, 3; Mus musculus; RNA-Seq
Total RNA was isolated using mirVana miRNA Isolation Kit (Ambion) following the manufacturer's protocol. 1.0 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were constructed using TruSeq Stranded mRNA LTSample Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer's instructions.