GSM6922577: sepcv096T10; Homo sapiens; RNA-Seq
2.5mL of whole blood was collected into PAXgene Blood RNA tubes (BD Biosciences; San Jose, CA, USA) and total RNA was extracted using the PAXgene Blood RNA Kit (Qiagen; Germantown, MD, USA). Samples with RNA Integrity Numbers less than 6.5 were discarded. Poly-adenylated RNA was captured using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB; Ipswich, USA). cDNA libraries were prepared using the KAPA Total RNA HyperPrep Kit (Roche; Basel, Switzerland) for Illumina sequencing.