GSM6755679: PD-L1 knock-out sample; Homo sapiens; RNA-Seq
The wild type and PD-L1-/- hPSC lines followed the macrophage differentiation . All cells were collected and resuspended in FACS buffer. Cells were stained by CD11b-APC-Cy7 and CD45-PE. After staining, cells were washed and resuspended in FACS buffer with DAPI. Cells were sorted using Fusion Flow Cytometer (BD Bioscience). DAPI-negative cells were sorted for Single-cell RNA-seq with the support of the Centre for PanorOmic Sciences (CPOS). the single-cell RNA-seq library was constructed and performed by Chromium Next GEM Single Cell 3ʹ Reagent Kit v3.1. Illumina Novaseq 6000 for Pair-End sequencing services was used and the final data output was 206.2 for PD-L1-/-  and 213.9G for wild type.