GSM7349861: expanded HSCs, CD201+CD150+CD48+KSL, rep1; Mus musculus; RNA-Seq
Over 5000 cells per population were sorted into 1.5 ml tubes and subsequently lysed in 600 µl Trizol LS reagent (Thermo Fisher Scientific). RNA purification, library preparation and next-generation sequencing was performed by Tsukuba i-Laboratory, LLC. Libraries were prepared using the SMARTer cDNA synthesis kit (Takara) and the high-output kit v2 (Illumina), followed by sequencing on a NextSeq 500 sequencer (Illumina) at 2x 36 paired end reads.