GSM7038936: Salivary glands, rep 1; Aedes aegypti; RNA-Seq
Afterwards, mosquitoes were dissected into tissue samples, including crop, fat body, leg, Malpighian tubules, midgut, ovary, salivary glands, and wings. Tissues from about 20 mosquitoes were pooled, homogenized in 1 mL RNA-Solv reagent (Omega Bio-Tek) for RNA isolation, using a Precellys 24 homogenizer (Bertin technologies). TruSeq Stranded mRNA kit (Illumina) was used for library preparation from total RNA according to the manufacturer's protocol.