GSM7864484: PBS, rep1; Mus musculus; RNA-Seq
RNA MiniPrep Kit (QIAGEN) RNAseq libraries were created from 400ng of total RNA using the NEBNext UltraII Directional RNA Library Prep kit (New England Biolabs) using half volume reagents and 17 cycles of PCR. Illumina library quality was confirmed using the Fragment Analyzer and qPCR