GSM8330659: 479_1; Homo sapiens; RNA-Seq
We enzymatically digested and dissociated fresh tumor samples according to tissue dissociation kit protocols using the gentleMACS™ Dissociator (Miltenyi). Dissociated tissues were filtered, centrifuged, and the isolated cells were resuspended in 0.1% BSA in PBS. Briefly, 5000-6000 cells were profiled per sample using the Chromium Single Cell 3' RNA reagent kit v3 according to the 10X Genomics protocol. The generated cDNA libraries were indexed, pooled, and sequenced in batches.