GSM7244865: dRNA-seq_∆rnc_+TEX_R1; Campylobacter jejuni; RNA-Seq
total RNA was treated with DNase I to digest genomic DNA, terminator exonuclease treatment of one half of each RNA sample small RNA protocol, as previously described (Berezikov et al., 2006), but without RNA fractionation prior to cDNA synthesis: Equal amounts of RNA were poly(A)‐tailed using poly(A) polymerase and 5'-triphosphate groups were digested with tobacco acid pyrophosphatase (TAP). Then, an RNA adapter was ligated to the 5' phosphate and first‑strand cDNA synthesised with an oligo(dT)‐adapter primer and M‑MLV (Moloney Murine Leukaemia Virus) reverse transcriptase. A high-fidelity DNA polymerase was used to PCR‐amplify the resulting cDNA to a concentration of ~20‐30 ng/μl. Finally, cDNA was purified with the Agencourt AMPure XP kit (Beckman Coulter Genomics) and analyzed by capillary electrophoresis.