GSM8291617: Mouse retina, 12mo; Mus musculus; RNA-Seq
Fresh retina from C57BL/6J (NNT mutation positive) mice were collected in AMES media +4%BSA and was digested using the Worthington papain dissociation system folloing the manufacturer's protocol. Cells were passed through a 30um filter (Miltenyi) to obtain a single cell suspension and enriched for RGCs and glia using Thy-1.2 and ACSA-2 (astrocyte cell surface antigen-2) antibodies respectively via magnetic-activated cell sorting (MACS®). Libraries were constructed according to manufacturer's protocol (10x Genomics Chromium Next GEM Single Cell 3' Kit v3.1 )