GSM7234120: Day4 (HH24) Chick Trunk_SingleCell; Gallus gallus; RNA-Seq
We harvested HH24 embryonic chick trunk tissue (2 somites in length; same  age/location as used in the Slide-seq and Visium experiments) from 6 embryos and  dissociated cells using a combination of mechanical and enzymatic steps (Morrison et  al., 2017) to generate a single cell suspension. Dissociated cells were loaded onto a  Chromium Single Cell Controller (10X Genomics), based on a live cell concentration of  700-1,200 nuclei/ul. cDNA libraries were prepared using the Chromium Single Cell 3'  Library and Gel Bead Kit v2 (10X Genomics) according to manufacturer's directions.  Resulting short fragment libraries were checked for quality and quantity using a  Bioanalyzer 2100 (Agilent Technologies) and Qubit Fluorometer (Invitrogen).