GSM8119640: Combined2; Homo sapiens; RNA-Seq
Total RNA was isolated using Trizol reagent (Invitrogen, Carlsbad, California, USA). RNA quality was assessed by Agilent TapeStation 4000 system (Agilent Technologies, Amstelveen, The Netherlands), and RNA quantification was performed using ND-2000 Spectrophotometer (Thermo Inc., Wilmington, Delaware, USA). Library construction was performed using the QuantSeq 3' mRNA-Seq Library Preparation Kit (Lexogen, Inc., Vienna, Austria) according to the manufacturer's instructions.  High-throughput sequencing was performed by single-end 75 sequencing using the NextSeq 550 (Illumina, Inc., San Diego, CA, USA).