GSM8427545: F1_3; Homo sapiens; RNA-Seq
Samples were snap-frozen in liquid nitrogen and stored at −80°C until needed. After five freeze-thaw steps in a 37°C water bath and liquid nitrogen, total RNA was extracted using TRIzol, then purified with the Direct-zol™ RNA Kit and treated with DNase I to remove possible DNA contamination. FASTQ output files were aligned to C. elegans WBcel269 reference genomes using STAR with a 2-pass procedure.