GSM8121738: Endosperm, WT; Oryza sativa; Bisulfite-Seq
Total DNA was extracted from the tissues using CTAB method. The DNA samples were sheared by ultrasonication (Covaris) avarage 350bp. Bisulfite convertion by EZ DNA Methylation-Gold Kit (Catalog no-D5005) as per manufacturer's protocol. Adapters used: Read1-AGATCGGAAGAGCACACGTCTGAACTCCAGTCA and Read2-AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT xGen™ Methyl-Seq DNA Library Prep Kit (Catalog no. 10009860)