GSM6500116: FBS-DUCAP.2-RNA/biol rep 2; Homo sapiens; ssRNA-seq
Total cellular RNA was extracted using the Norgen RNA Purification PLUS kit (#48400, Norgen Biotek Corp) as per manufacturer's instructions. RNA quality (RIN>8) and quantity were determined on an Agilent 2100 Bioanalyzer (Agilent Technologies) and Qubit®. 2.0 Fluorometer (Thermo Fisher Scientific). RNA libraries for RNA-seq were prepared using the Illumina TruSeq Stranded mRNA Sample Prep Kit (strand-specific, polyA enrichment), including ERCC controls.