GSM8292065: K562, WT, H3K4me1, rep1; Homo sapiens; ChIP-Seq
Cells were crosslinked with 1% formaldehyde for 10 minutes. Chromatin was sheared by sonication. Immunoprecipitation was performed overnight with the corresponding primary antibody. DNA was purified from A/G beads using phenol-chloroform. ChIP-seq libraries were prepared using NEBNext Ultra Library Prep Kit for Illumina according to the manufacturer's conditions.