GSM8162752: Brain, 2 Months, Rep6; Mus musculus; RNA-Seq
RNA was extracted from tissue samples of liver, colon and left brain hemisphere with the phenol-chloroform extraction method using for each tissue 1ml Qiazol Lysis Reagent (Qiagen, Hilden, Germany). RNA was reverse transcripted into Illumina shotgun sequencing libraries at Competence Centre for Genomic Analysis (CCGA, Kiel Germany) with TruSeq RNA stranded kit (Illumina, San Diego, CA) with polyA enrichment according to the manufacturer's instructions and sequenced for 2x75 cycles (2x100 for colon) in paired end mode with ~13 samples per lane on a HiSeq4000 machine. Demultiplexing was done with zero mismatches allowed in the barcodes.