GSM7159066: Somatosensory cortex, Ctrl, H3K36me2, rep2; Mus musculus; ChIP-Seq
Somatosensory cortices were digested into single cells by Papain. Nuclei were extracted in lysis buffer [10 mM Tris pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% (v/v) Igepal CA-630)]. Nuclear extracts were fixed with 1% formaldehyde for 10 minutes. After digested by micrococcal nuclease, cell lysis was sonicated in EP tubes with power output 100 W, 5 min, 0.5 s on, 0.5 s off on ice. ChIP-seq libraries were constructed by VAHTS Universal DNA Library Prep Kit for Illumina V3 (Vazyme ND607). 5 ng of DNA was used as starting material for input and ip samples. Libraries were amplified using 10 cycles on the thermocycler. Post amplification libraries were size selected at about 300 bp in length using VAHTS DNA Clean Beads (Vazyme N411). The concentration and quality of DNA was measured with an Agilent 2100 Bioanalyzer.