GSM8330900: Trachea_E15.5_mut_rep3; Mus musculus; RNA-Seq
For RNA extraction, embryonic tissues were dissected into ice-cold PBS, and either homogenized immediately in Trizol (Invitrogen), following manufacturer's instructions or snap-frozen in liquid nitrogen until further processing. mRNA were isoleted by polyA selection from 500ng to 1000 ng of total RNA. Lbrary were made using Illumina TruSeq RNA prep kit