GSM6755833: Vehicle_Astro_drops_3; Mus musculus; RNA-Seq
In vivo: Each CNS was isolated per mouse. A cell suspension was generated by papain digestion, neutralization, and 70um filtration of cell extract. Cells were centrifuged, resuspended in 30% Percoll, centrifuged, and washed in PBS. Cells were sorted by flow cytometry based on a negative selection strategy for astrocytes. In vitro: Cells from culture were lysed in RLT and processed by Qiagen RNeasy kit protocol. Libraries were prepared by NEBNext Low Input kit.