GSM6807973: FOXP3 KO MT-2 biol rep 3; Homo sapiens; RNA-Seq
Total RNA was isolated using Zymo Direct-zol RNA MiniPrep with TRI Reagent miRNeasy kits from Zymo Research (Irvine, CA, USA) according to the manufacturer's instruction with the following modifications: 1) snap-frozen cells were lysed in at least 200 mL of TRI reagent without thawing cells; 2) cells were not centrifuged to collect debris before applying to column. ALl samples were eluted in 25 mL of DNase/RNase-Free water. RNA libraries were prepared for sequencing using standard Illumina protocols.