GSM8257006: MCF7 cells; WT ER; Fulvestrant (10nM); biol rep 1; Homo sapiens; RNA-Seq
Then, cells were harvested (as duplicates) for downstream RNA analysis. Total RNA was extracted using the QIAGEN RNeasy kit with DNase digestion. RNA concentrations were measured by NanoDrop, and the quality of RNA was determined by a Bioanalyzer. RNA-seq libraries were made using the TruSeq RNA Sample Preparation Kit (Illumina). Samples were sequenced on an Illumina Nextseq500. RNA-seq libraries were made using the TruSeq RNA Sample Preparation Kit (Illumina).