GSM8254991: Single cell, Hippocampus, Metll3CKOE-YTH; Mus musculus; RNA-Seq
For mouse hippocamus collections, hippocampi were dissected 2 weeks after AAV injectsions; for single cell analysis, cells were dissociated 2 weeks after AAV injections with using the Adult Brain Dissociation Kit (Miltenyi Biotex no. 130-107-677); For single cell sequencing: Briefly, after hippocampi dissociation, cells were FACS sorted and live cells were collected in ice-cold PBS containing 0.5% BSA. Per sample, 30,000 cells were loaded into a Rhapsody Cartridge (BD) and processed following the manufacturer's instructions. Subsequently, single cell RNA sequencing libraries were prepared using the Rhapsody WTA kit (BD). for single cells: Rhapsody WTA kit (BD)