GSM6481515: MICE28; Mus musculus; RNA-Seq
Lung lobes were stored in RNA later solution (Invitrogen) for 48 hours to stabilize RNA. Next, tissues were disrupted using TissueRuptor II (Qiagen) in RNeasy Lysis Buffer (Qiagen). Total RNA was isolated by using the RNeasy Mini Kit according to the manufacturer's protocol (Qiagen). Only the RNA with proper RNA integrity, namely RIN>8, was subjected to cDNA library establishment according to TruSeq Stranded Total RNA protocol (Illumina), followed by the quality confirmation (Agilent Technologies 2100 Bioanalyzer). The expected length of the final product was assumed at 260 base pairs. RNA libraries were prepared for sequencing using standard Illumina protocols