GSM8291862: LF1 cells, quiescent, replicate 1; Homo sapiens; RNA-Seq
Total RNA for proliferating and RS samples (extracted using trizol) and cell pellets for quiescent cells were sent to Azenta. where libraries were prepared using a stranded ribosomal depletion method. The proliferating and RS cells were split across two HiSeq lanes. The quiescent cells were run on one HiSeq lane. NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®