GSM8070989: TACC1.RARg.M.AR.3.sorted.rmdup.BAM; Homo sapiens; OTHER
Harvested cells were washed with pre-activated Concanavalin A-coated beads mixed with antibody overnight at 4°C. CUTANA pAG-MNase (EpiCypher) was incubated with sample for 10 minutes at room temperature. Sequencing libraries prepared from immunopreciptiated DNA Fragmented DNA samples was purified using the Monarch DNA Clean up Kit (New England Biolabs). The libraries were prepared from 5-10ng purified CUT&RUN-enriched DNA, using the NEB Ultra II Library Prep Kit (New England Biolabs) per manufacturer's instructions and sequenced using Hiseq 4000 PE 150bp. Transcription factor modifications for sub-nucleosomal size (<120bp) DNA fragments were undertaken as per protocol.