GSM7622302: Polycomb knockdown IgG; Mus musculus; OTHER
Cut and run was performed utilizing the CUTANA™ ChIC/CUT&RUN Kit (Epicypher, 14-1048). In brief, 0.25 million cells per sample were collected via trypsinization and captured with activated ConA beads. Cells were permeabilized with 0.05% digitonin. 0.5ug antibody targeting the HA tag (Epicypher, cat. No. 13-2010) on Ring1B was used. IgG (negative) and H3k4me3 (positive) control antibodies were included in the kit.  DNA was cleaved with PAG-MNASE and released to solution. Purified DNA was library prepped with the  CUTANA™ CUT&RUN Library Prep Kit (Epicypher, cat. No. 14-1001).