GSM8332110: D. melanogaster vigEP812 +/-, testis; Drosophila melanogaster; ncRNA-Seq
Small RNAs of 18–29 nt in size from gonads of D. melanogaster vasEP812-/-, vasEP812+/-, vasEP812-/-; rhi-GFP, vasEP812/vasD1, mix of vasEP812/+ with vasD1/+, and vasEP812/vasD1; rhi-GFP were isolated with TRIzol Reagent (Invitrogen). Small RNA fraction (19–29 nt) was purified by 15% polyacrylamide gel libraries were prepared using NEBNext Multiplex Small RNA Sample Prep Set for Illumina (E7300S) and sequenced on NovaSeq 6000 in 50 bp single-end mode.