GSM7623447: Normal Aminocytes, MX1_OE, rep2; Homo sapiens; RNA-Seq
Total RNA of male aminocytes from different group was extracted using TRIzol (Invitrogen, Carlsbad, CA,USA) according to the manufacturer's instructions. total RNA was quantified using an Agilent 2100 bioanalyzer (Thermo Fisher Scientific, USA) and purified using oligo(dT)-attached magnetic beads. The library was constructed, and single-end 50-base reads were generated on the BGIseq500 platform (BGI-Shenzhen, China). Raw data were filtered with SOAPnuke to remove adaptor sequences, unknown reads containing polyN sequences, and low-quality reads, thus obtaining clean reads and stored in FASTQ format.