GSM8291542: LNA2 treated samples replicate 1; Homo sapiens; RNA-Seq
RNA was isolated using the RNeasy Mini Kit (QIAGEN). The removal of genomic DNA was confirmed in-column with DNase treatment (RNase-Free DNase Set; QIAGEN) for 15 min, followed by an additional treatment of the samples with DNase I (Invitrogen). Nanodrop 2000 and BioRad Experion were used to measure the quantity and quality of RNA, respectively. Libraries for RNA-Seq were prepared using Illumina Stranded mRNA Library Preparation, Illumina (1000000124518). The quality of the libraries was confirmed with Advanced Analytical Fragment Analyzer, and the concentrations of the libraries were quantified with Qubit® Fluorometric Quantitation (Life Technologies). Nine samples pooled on one and sequenced on  one lane with 2x150bp read length. Sequencing was performed at the Finnish Functional Genomics Centre using the NovaSeq 6000 SP v1.5 Next-Generation Sequencing platform.