GSM7440353: DoHH2 iCLIP rep2; Homo sapiens; RIP-Seq
40x10^6 cells crosslinked using 150 mJ/cm2 UVC. RNA was partially digested with RNase I (0.6 U) prior to immunoprecipitation with 10 ug anti-eIF4B antibody. Immunoprecipitated RNA was 5'-end-labeled with 32P using T4 polynucleotide kinase and protein-RNA complexes were separated by SDS-PAGE and transferred to nitrocellulose membrane. RNA fragments bound to eIF4B were isolated by proteinase K digestion and phenol-chloroform separation. Homemade with custom clip primers.