GSM6422863: SAG, replicate 1, scRNAseq; Mus musculus; RNA-Seq
Freshly collected SMGs at day 4 after SAG treatment were minced and digested for 1 h with RPMI 1640 medium containing 1 mg/ml mg/mL collagenase IV, 5mM CaCl2, 50 mg DNase I, and 8% fetal bovine serum with continuous shaking at room temperature. scRNA-seq libraries were generated using the 10x Chromium platform and the version 3 Chromium Single Cell 3` reagent kit following the manufacturers instructions (10x Genomics).