GSM7349912: iWAT22_2_IP; Mus musculus; ChIP-Seq
iWAT tissues were cut and fixed with 1% formaldehyde and stopped by adding glycine. Pellets were homogenized and resuspended in lysis buffer, and crosslinked chromatin was sheared into 200-500 bp fragments with Bioruptor Sonication System UCD-300 . 200 ug DNA from each sample were resuspended in digestion buffer containing H1.2 antibody plus protein G magnetic beads for overnight. Immune complexes were collected, washed, and eluted. The DNA-protein crosslinks were then incubated overnight at 65 °C for extracting DNA for qPCR analysis or high through-put sequencing. The high-throughput DNA sequencing libraries were prepared by using VAHTS Universal DNA Library Prep Kit for MGI（Catalog NO. NDM607, Vazyme）. The library products corresponding to 200-500 bps were enriched, quantified and finally sequenced on DNBSEQ-T7 sequencer (MGI Tech Co., Ltd. China) with PE150 model.