GSM8208521: 26; Homo sapiens; miRNA-Seq
total RNA extracted from purified exosomes was examined using agarose electrophoresis and quantified using nanodrop, respectively. MiRNA library was constructed with NEB Multiplex Small RNA Library Prep Set for Illumina (Illumina, USA) based on the manufacturer's instructions. The quality of sequencing libraries was tested with Agilent 2100 Bioanalyzer. Libraries were mixed and degenerated into single-stranded DNA with 0.1M NaOH. The mixed samples were sequenced 50 cycles with Illumina NextSeq 500 (Illumina, USA).