GSM6578822: SEc6-D9; Mus musculus; RNA-Seq
Each animal was perfused with 20 ml of cold PBS, and then 1/2 of the kidney was sampled and quicly placed in media with 2% FBS on ice. After micing with razor blade, the remainings were transferred to 1X Liberase TH (Millipore Sigma) dissociation medium and subjected to single cell dissociation.