GSM7734551: BAT-WT4-IGG; Mus musculus; RIP-Seq
Total RNA was extracted using Trizol reagent The RNA immunoprecipitation assay was carried out with GenSeq® RIP kit (GenSeq Inc., China) according to the manufacturer's instructions. rRNAs were removed from the immunoprecipitated RNA and input RNA samples by using NEBNext rRNA Depletion Kit (New England Biolabs, Inc., Massachusetts, USA). RNA libraries were constructed by using rRNA-depleted RNAs with NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England Biolabs, Inc., Massachusetts, USA) according to the manufacturer's instructions.