GSM7235313: SEM_PINO_RES_DOT1Li_H3K4me3; Homo sapiens; ChIP-Seq
Up to 20 million leukemic cells were crosslinked and lysed using the SimpleChIP kit (Cell Signaling Technology®, #9003) according to the manufacturer's recommendations and subsequently sonicated using a Bioruptor sonicator (Diagenode) to generate 150-300 bp fragment size. Next, immunoprecipitation and antibody-protein-DNA precipitation were performed according to the guidelines of the manufacturer.   Antibodies against KMT2A (Bethyl labs; cat.nr. A300-086-A), AFF1 (Abcam; cat.nr. ab31812), H3K4Me3, H3K27Ac and H3K79Me2 (Diagenode; cat. nrs. pAB-003-050, C15410196 and C15410051) were used. ChIP-seq DNA libraries were generated using the NEB Next Ultra DNA library preparation kit for Illumina (New England Biolabs) according to the manufacturer's recommendations ChIP-seq DNA libraries were generated using the NEB Next Ultra DNA library preparation kit for Illumina (New England Biolabs, E7645) according to the manufacturer's recommendations