GSM7158697: WT dsDNA+anti-IFNARab, 4 biol replicates (cell culture), scRNA-seq; Mus musculus; RNA-Seq
Femurs and tibia were harvested from C57BL6 or IFNAR-/- mice. Bone marrow cells were flushed from femurs with ice-cold PBS and filtered through a 40 µm nylon mesh. The filtered solution was treated with red blood cell (RBC) lysis buffer (BioLegend). Bone marrow cells were flushed from femurs with ice-cold PBS and filtered through a 40 µm nylon mesh. The filtered solution was treated with red blood cell (RBC) lysis buffer (BioLegend). Single cell libraries were prepared according to standard 10X Genomics protocols Dual index Illumina single cell RNA sequencing