GSM7697869: C73_B1_VISIUM; Homo sapiens; OTHER
Tissue was prepared for Visium Spatial transcriptomics as previously described (https://doi.org/10.1002/hep4.1854)]. Briefly, liver tissue was embedded in OCT media, frozen, and cryosectioned with 16-um thickness at -10ºC (cryostar NX70 HOMP). Sections were placed on a chilled Visium Tissue Optimization Slide (10x Genomics) and processed following the Visium Spatial Gene Expression User Guide. Tissue was permeabilized for 12 minutes, based on an initial optimizations trial. Libraries were prepared according to the Visium Spatial Gene Expression User Guide and samples sequenced on a NovaSeq 6000. Spatial transcriptome sequencing libraries were prepared with 10X Genomics Visium Spatial Gene Expression Slides & Reagent kit according to manufacturer's construction