GSM8119677: KO-D4-2; Mus musculus; RNA-Seq
Tissues were stored in the sCelLiveTM Tissue Presevation Solution (Singleron Biotechnologies, Nanjing, China) and then dissociated into sinled cell suspension with a Singleron PythoNTM Automated Tissue Dissociator with sCelLiveTM Tissue Dissociation Mix.Then single cell suspensions were prepared and loaded onto microfluidic devices. Library are constructed by using GEXSCOPE Single cell RNA Library Kit according to Singleron GEXSCOPE protocol