GSM6533824: PA01-H4K8ac-rep3-IP; Caenorhabditis elegans; ChIP-Seq
The worms were exposed to P. aeruginosa PA01 on PGS plates at 25 °C for 12 hours. After three washes in M9 buffer, animals were resuspended in PBS, frozen immediately in liquid nitrogen followed by storage at -80 °C. After cell lysis, the chromatin was sheared into 0.2- to 1-kb fragments. The soluble chromatin fraction was immunoprecipitated using antibodies specific for H4K5ac (Abcam, ab51997) or H4K8ac (Abcam, ab45166). ChIP-seq libraries were prepared using the NEBNext UltraDNA library prep kit (New England Biolabs) and single-end sequenced on an Illumina HiSeq6000.