GSM8291576: Uranus (iPSC line #2); Homo sapiens; RNA-Seq
freshly harvested iPSC-macrophages from the differentiation plate. Library preparation for single cell mRNA-Seq analysis was performed according to the Chromium NextGEM Single Cell 3ʹ Reagent Kits v3.1 User Guide (Manual Part Number CG000204 Rev B; 10x Genomics). According to the protocol, a given excess of cells was loaded to the 10x controller in order to reach a target number of 4,000 cells per sample. Fragment length distribution of generated libraries was monitored using 'Bioanalyzer High Sensitivity DNA Assay' (5067-4626; Agilent Technologies). Quantification of libraries was performed by use of the 'Qubit® dsDNA HS Assay Kit' (ThermoFisher Scientific #Q32854).