GSM7698141: P5-N3; Human alphaherpesvirus 1; RNA-Seq
HeLa cells were infected with the YK410 strain at a multiplicity of infection (MOI) of 5 by incubating for 24 h. The cells were then washed with phosphate-buffered saline (PBS) and detached with 0.25% trypsin/EDTA solution (Wako). Then, 5 x 10^5 cells from the f1–f6 subpopulations were sorted in PBS using a BD FACSMelodyTM Cell Sorter (Becton Dickinson). Following centrifugation, the total RNA was isolated from the fractionated cells with a High Pure RNA Isolation kit (Roche). Full-length cDNA was prepared using a SMART-Seq HT Kit (Takara Bio, Japan) according to the manufacturer's instructions. According to the SMARTer kit instructions, an Illumina library was then prepared using a NexteraXT DNA Library Preparation Kit (Illumina).