GSM8292040: HEK293T_siLa_rep3; Homo sapiens; ncRNA-Seq
small RNA extraction was performed using mirVana PARIS RNA and Native Protein Purification kit according to the manufacturer's instructions The samples were first treated with Antarctic Phosphatase (NEB), followed by treatment with T4 Polynucleotide Kinase (NEB) according to the manufacturer's protocol. Small RNA libraries were generated using the NEBnext small RNA library preparation kit according to instructions (New England BioLabs).