GSM8254985: Input2; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. Chromatin was sonicated using Diagnode bioruptor pico sonicator after quenching and cell lysis. The purified DNA was used for ChIP-seq library preparation. ChIP-Seq library preparation was performed by Novogene Corporation Inc (China). After the construction of the library, initial quantification was done with Qubit 2.0 and the library was diluted to 1ng/l. The insertion size of the library was detected with NGS3K.