GSM8070717: 106D; Mus musculus; OTHER
At 4 (CPZ) and 6 weeks (Control and Recovery), mice were euthanized by carbon dioxide (CO2) inhalation, and their brains were subsequently dissected. Each brain was sagittally cut into left and right hemispheres. One hemisphere was then further divided into three coronal segments, which were embedded in OCT for spatial transcriptomic analysis. Frozen sections of mouse brain hemispheres embedded in OCT (TissueTek) were collected using a Leica CM1520 cryostat equipped with a Leica Surgipath high-profile blade at -23°C. The 10 µm tissue sections were placed onto Visium Spatial Gene Expression slides (10X Genomics Slide Kit, Ref No. 1000185). The tissue sections were processed using the 10X Genomics Visium platform following manufacturer's instructions. Library construction was carried out using the 10X Genomics Library Construction Kit (PN-1000190) following the manufacturer's instructions. The libraries were then sequenced on an Illumina NovaSeq 6000 S2 flowcell, employing paired-end sequencing with the parameters Read1-28bp, i7-10bp, i5-10bp, and Read2-120bp.