Illumina NovaSeq 6000 paired end sequencing; Human fetal liver CITE-seq data
Samples were collected by the Human Developmental Biology Resource from the clinic and transported to the lab immediately before dissection. Livers were digested for30 mins using collagenase type 4 to create a single cell suspension. Nucleic acids were extracted following the manufacturers guidelines for the 10x Genomics Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry) (CG000204_ChromiumNextGEMSingleCell3'v3.1_Rev D.pdf).CITE-Seq libraries were amplified following the TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3' Reagent Kit v3 or v3.1 (Single Index) Protocol (https://www.biolegend.com/en-us/protocols/totalseq-a-antibodies-and-cell-hashing-with-10x-single-cell-3-reagent-kit-v3-3-1-protocol). Nucleic acid libraries were constructed following the manufacturers guidelines for the 10x Genomics Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry) (CG000204_ChromiumNextGEMSingleCell3'v3.1_Rev D.pdf).CITE-Seq libraries were constructed following the TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3' Reagent Kit v3 or v3.1 (Single Index) Protocol (https://www.biolegend.com/en-us/protocols/totalseq-a-antibodies-and-cell-hashing-with-10x-single-cell-3-reagent-kit-v3-3-1-protocol).