GSM7233777: V_8_1_5, p601, day8, DonorII, H3K27me3; Homo sapiens; ChIP-Seq
Native chromatin immunoprecipitation (nChIP) was conducted as described (Brind'Amour et al., 2015), using a starting material of 200,000 nHDFs per IP. The following antibodies were used for the nChIP reactions: rabbit mAbs α-H3K4me3 (1:200; Merck Millipore), α-H3K27me3 (1:300; Cell Signaling), α-H3K9me3 (1:200; Active Motif), α-H3K27ac (1:200; abcam) and IgG (1:200; Merck Millipore). For sequencing of extracted DNA, libraries were prepared using the NEXTFlexTM ChIP-Seq Library Prep Kit (Bioo Scientific) according to the manufacturer's instructions. Samples were sequenced on an Illumina HiSeq2500.