GSM6756150: TCRa-CD8+TRM cells from brain-2; Mus musculus; RNA-Seq
After 5~7days of rechallenge of GBM (DBT) cells in brain,leukocytes from brain and draining lymph nodes were stained with anti-CD3, anti-CD44, and anti-CD8 antibodies for 30 minutes at RT and washed twice. Then, live CD3+CD44+CD8+ T cells were sorted by using a FACSAria III flow cytometer (BD Biosciences) into 15-mL sterilized tubes containing 2 mL RPMI 1640 medium. After the samples were spun down, the supernatant was discarded. Next, the CD44+CD8+ T cells were resuspended in 1 mL RNAprotect Cell Reagent (Qiagen Inc.) Library generated with iRepertoire's reagent systems for a  specific chain and molecular barcode. Amplified libraries-TCRa, TCRb were used here. Amplified libraries-TCRa, TCRb-RNA-seq