Illumina HiSeq 2500 sequencing; High throughput targeted RNA sequencing on RPTEC/TERT1 cell line exposed to multiple concentration levels of cyclosporine A at various different time points
Wash the cells with PBS 1 times. Add the 1x BioSpyder lysis buffer. Incubate at the room temperature for 15 mins. Store the lysates at -80'C until sequencing. Nucleic acid extraction was done following the Tempo-seq protocol detailed here: https://www.frontiersin.org/articles/10.3389/fgene.2018.00485/full#h3 Multiple barcoded samples are pooled into a single library and sequenced on an Illumina HiSeq 2500 Output v4 flow cell. The sequencing is single end and reads are expected to be 68bp in length. Sequencing reads are demultiplexed to create a single FASTQ file per sample.