GSM8332089: 6419C5 parental replicate 1; Mus musculus; RNA-Seq
Parental and MRTX1133 resistant cell lines were trypsinized and washed with PBS twice, cell pellets were collected, and total RNA was extracted using the RNeasy Plus Kits for RNA Isolation according to the manufacturer's protocol (Qiagen; Cat. #73404). Total RNA was sent to Novogene (Sacramento, CA) for mRNA sequencing library preparation and next generation sequencing using Illumina NovoSeq. All the conditions and cell lines were prepared in triplicate. Library preparation conducted by Novogene Corporation: mRNA enrichment by (poly)A capture, and then fragmented mRNA into 100-200nt using enzyme digestion, and final step with cDNA reverse transcription and PCR to add sequencing adaptor to the library.