GSM6859099: 1-month Terc; Mus musculus; RNA-Seq
Bone marrow was flushed and kept in MACS Tissue Storage Solution (Miltenyi Biotec). Samples were sieved through 40µm cell strainers, and centrifuged at 300 g for 5 min. Pelleted cells were suspended in red blood cell lysis buffer (Miltenyi Biotec) for lysing red blood cells.Brain tissues were surgically removed and minced into small pieces (approximately 1mm3) on ice and digested by 200 U/mL Papine (Diamond) and Cysteine-HCL (Sigma). Then samples were sieved through 70 µm cell strainers, centrifuged at 300 g for 10 min, and further cleaned for red blood cells using red blood cell lysis buffer (Miltenyi Biotec). RNA libraries were generated by 10xGenomics Single Cell 3' Library Gel Bead Kit V3 and prepared for sequencing using standard Illumina protocols