GSM6533783: WS, brain organoid, day56 [RNA-seq]; Homo sapiens; RNA-Seq
Human organoids were homogenized in TRIzol reagent (Thermo Fisher Scientific) to extract total RNA and was purified with chloroform following the manufacturer's protocol. The concentration of RNA was quantified using a NanoDrop spectrophotometer 2000 (Thermo Fisher Scientific). RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.