GSM6965199: OPC Ro1138452, 2; Homo sapiens; RNA-Seq
Cells from each well were dissociated into single cells by treating with accutase for 45 minutes (just the cells that had migrated out from the spheres were collected, the spheres remain mostly intact). Single cells were passed through 40 uM cell strainers, washed once with PBS, cryopreserved in CTLC-ABC (NC9711917, Immunospot) buffer, and stored in -80 before shipping for single cell capture The cryopreserved cells were thawed by following the instructions provided for CTLC-ABC. Cells were washed and counted on the DeNovex cell counter. Single cell capture, barcoding, post GEM-RT cleanup, cDNA amplification, and 3' library construction was performed using the 10X genomics' single-cell RNA-seq technology by following their detailed instructions