GSM7864724: E16 Tbx4rtta; tetocre; Sin3a F/F, CKO; Biol rep 1; Mus musculus; RNA-Seq
RNA was harvested from sorted mesenchymal cells using the TRIzol (Fisher) RNA extraction protocol and analyzed using Aligent Bioanalyzer RNA kit. cDNA libraries were made using the 10x Genomics Single Cell 3' v3.1 Reagent's Kit (10x Genomics) following manufacturer's protocols. Briefly, Gel Bead-In-Emulsions (GEMs) were created using the Chromium Controller instrument (10x Genomics) and GEM-RT was performed using an Eppendork Mastercycler Pro thermal cycler. GEMs collected and cDNA amplified using Aglient Bioanalyser High Sensitivity chip. Duel indexed sequencing libraries were created using Chromium Single-Cell 3' Library Construction Kit, and barcoded libraries were quantified using Qubit High Sensitivity DNA reagents (Invitrogen Qubit). Barcoded libraries were analyzed on a Aglient Bioanalyzer DNA 1000 chip.