GSM7234154: MLS_2645-94_3D_2; Homo sapiens; RNA-Seq
Scaffold samples were lysed in RLT lysis buffer and homogenized in a Qiagen TissueLyzer II using stainless steel beads. 2D samples were lysed in RLT lysis buffer or QIAzol lysis buffer. RNA was extracted from all samples using Qiagen RNeasy micro kit or miRNeasy micro kit, including DNase treatment. The library was generated using the Smart-seq2 protocol (Picelli, Faridani et al. 2014) with some modifications.