GSM6922822: Untreated-control_1; Homo sapiens; RNA-Seq
Total RNA was extracted and purified according to the manufacturer's instructions using TRIzol (Thermo Fisher Scientific) and RNA Clean and Concentrator-5 kit (Zymo Research). The concentrations of extracted RNA were determined by using a NanoDrop OneC spectrophotometer (Thermo Fisher Scientific) and Qubit fluorometer (Thermo Fisher Scientific). The integrity of RNA was checked using an automated electrophoresis system, 4200 Agilent TapeStation (Agilent).