GSM7349794: mESC_E14_shSox2_rep1; Mus musculus; RNA-Seq
Cells were used to extract the total RNA using the Total RNA Extraction Kit (Omega). Oligo (dT)-attached magnetic beads were used to purified mRNA. The final library was amplified with phi29 to make DNA nanoball (DNB) which had more than 300 copies of one molecular, DNBs were loaded into the patterned nanoarray and paired end 150 bases reads were generated on MGISEQ-2000 platform (BGI-Shenzhen, China).