GSM7734607: CHLA-02, DAPT, replicate 3; Homo sapiens; RNA-Seq
RNAs were extracted using the Nucleospin II kit (Macherey-Nagel). The tumor cell content was visually estimated before RNA extractions. Barcoded Illumina compatible libraries were generated from 750 ng of total RNA for each sample using TruSeq Stranded mRNA Library Preparation Kit (Illumina, San Diego, California, U.S.,). Libraries were sequenced using the illumina NovaSeq platforms in the 100 bp paired-end mode.