GSM7242373: Mouse colonic organoids, Tnfr2-/-, Untreated; Mus musculus; RNA-Seq
At 7 days post-passage, organoid cultures were dissociated with a 10-minute incubation with 1X TrypLE at 37 degrees C. Cells were passed through a 40-micron nylon mesh. The single-cell suspension was depleted of apoptotic cells using the Annexin V Dead Cell Removal Kit (StemCell Technologies). Single-cell 3' libraries were generated using the 10X Chromium platform. Paired-end sequencing was performed on a HiSeq 4000.