GSM7158847: 60nt_oligo, no_CMC_treated, blocked, no_XRN1_treated, rep2; synthetic construct; OTHER
The oligonucleotides were purchased. RNA oligonucleotides were RNA was reverse transcribed by Superscript III reverse transcriptase (Thermo scientific). The  5' adaptor was ligated by T4 RNA ligase high concentration 1 (NEB). The amplification cycles of the cDNA were determined by qPCR (generally 10-15 cycles). The cDNA was amplified by PCR with Q5 High-Fidelity 2×Master Mix (NEB). Library PCR products were purified twice by 0.9x XP beads (Beckman).