GSM6965672: p1-1dpi; Astyanax mexicanus; RNA-Seq
RNA from skeletal muscle was extracted using standard phenol/chloroform extraction. The subsequent RNA pellet was cleaned with the RNeasy Mini Kit (Qiagen #74104) with on-column DNAse digestion (Qiagen #79256). Libraries were prepared according to the manufacturer's instructions using the TruSeq Stranded mRNA Prep Kit (Illumina #20020594) The resulting libraries were quantified using a Bioanalyzer (Agilent Technologies) and Qubit fluorometer (Life Technologies). Libraries were normalized, pooled, multiplexed, and sequenced on an Illumina NextSeq-75-HO instrument as v2 Chemistry High Output 75bp single read runs.