GSM8070942: tdLN PIB, TCR; Mus musculus; OTHER
Single cells were isolated using 10x Chromium by 10x Genomics. scRNA-seq libraries were prepared with Chromium Next GEM Automated Single Cell 5' Kit (10x Genomics) according to manufacturer's instructions. TCR libraries were prepared with Chromium Automated Single Cell Human TCR Amplification & Library Construction Kit(10x Genomics) according to manufacturer's instructions. Library size and quality check was performed using High Sensitivity D1000 ScreenTape with High Sensitivity D1000 Reagents (Agilent) in a 4200 TapeStation System (Agilent), according to the manufacturer's protocol.