GSM7917967: RAW264.7, oxldl, C1; Mus musculus; ATAC-seq
5 x 104 cells were washed twice in cold PBS and resuspended in 50μl of cold lysis buffer (10mM Tris-HCl, pH 7.4, 10mM NaCl, 3mM MgCl2, 0.1% Tween, 0.1% NP40 and 0.01% Digitonin) Lysates were centrifuged (750 x g, 10min, 4°C) and nuclei were resuspended in 50μl of transposition reaction mix with 5 μl Tn5 Transposase (TruePrep DNA Library Prep kit V2 for Illumina) and incubated for 30 min at 37°C. Transposed DNA fragments were purified using a QIAGEN Reaction MiniElute Kit, barcoded with Novo NGS Index Kit for Illumina and amplified to 240 fmol by NEBNext Ultra II Q5 Master Mix. PCR products were purified using a PCR Purification Kit  for paired-end sequencing in Novaseq 6000.