GSM8257125: liver, BRG1/BRM, rep1, RNA-seq; Mus musculus; RNA-Seq
10 mg of snap-frozen liver tissue was homogenized in 700µL Tri Reagent (Sigma Aldrich, 93289) using the FastPrep-24™ 5G Instrument. 0.2 volume chloroform was added, and the sample was vortexed for 30 sec, incubated for 15 min at RT, and centrifuged at 10,000g for 10 min at 4 °C. The upper phase was transferred to a new tube and 0.6 volume 96% ethanol was added. The sample was loaded into an EconoSpin column (Epoch, 1920-050/250), centrifuged at 13000g for 30 sec. Flowthrough was discarded and the column was washed 3 times with buffer RPE (Qiagen, 1018013) by adding 500µL of buffer RPE, centrifuging, and discarding flowthrough. After washing, the column was centrifuged at 13000g for 2 min at RT to dry. The column was transferred to a new Eppendorf tube and incubated with open lid for 5 min to evaporate remaining ethanol. 30µL of DEPC-treated water was pipetted to the center of the membrane, incubated for 2 min at RT and centrifuged at 16000g for 1 min.  RNA concentration was determined using NanoDrop ND-1000 Spectrophotometer. RNA quality was assessed via a standard sensitivity RNA kit (Agilent, DNF-471) and fragment analyzer. RNA integrity number (RIN) > 8.0 was considered good quality. Libraries from RNA using NEBNext® reagents and kits for Illumina® Sequencing following manufacturer's instrcutions. Samples were sequenced on an Illumina NovaSeq 6000 instrument.