GSM8292109: OC_P8SC_DTR_rep6; Mus musculus; RNA-Seq
Organs of Corti from p1 LfngeGFP were dissected from p1 LfingGFP mice and cultured in the presence of 3 um DMSO or 3 uM TDI-000536 LKI for five days. The explants were then dissociated into a single cell suspension and eGFP-positive supporting cells were purified via fluorescence-activated cell sorting (FACs). Alternatively, supporting cells were purified from primary organs of Corti dissected from p8 LfngCreERT2/Rosa26LSL-tdTomato/Pou4f3+/+ or LfngCreERT2/Rosa26LSL-tdTomato /Pou4f3DTR/+  mice treated with tamoxifen to label supporting cells at p2 and diphtheria toxin to ablate hair cells at p4. Cells were FAC-sorted directly into 300 uL of lysis buffer and RNA was immediately extracted from using the Quick-RNA MicroPrep Kit (Zymo Research R1050). After passing quality controls, RNA was used for library preparation using with QIAseq FX Single Cell RNA Library Kit and sequenced using HiSeq 4000.