GSM6756032: HBL1WT1; Homo sapiens; RNA-Seq
RNA-seq was performed on three biological replicates for each cell line (DOHH-2, OCI-LY1, HBL-1, OCI-LY3). In brief, total RNA was extracted from 2 x 106 cells (pre-treated with serum-starved culture medium for 12 hr) using a commercial column-based purification kit (Qiagen, Hilden, Germany) with on-column DNase treatment. Following QC and quantification, 500 ng input RNA was prepped using the TruSeq Stranded mRNA kit and ran on a NextSeq550 instrument (Illumina, San Diego, CA).