GSM6965581: Blood, ParticipantG, 08.00h; Homo sapiens; RNA-Seq
Total RNA was extracted using the “Preserved Blood RNA Purification Kit I (for use with Tempus Blood RNA tubes)” (Norgen, Thorold, Canada, Cat. No. 43400) according to a modified version of the manufacturer's instructions in which the amount of Tempus Blood RNA Tube Diluent was downscaled to a sample volume of 100 µl blood and 200 µl Tempus™ RNA Blood stabilization solution. The RNA was finally eluted in a volume of 30 µl Elution Solution and residual genomic DNA was removed by treatment with the TURBO DNAse free kit (Thermo Fisher Scientific, Massachussets, USA, Cat. No. AM1907). Library preparation including rRNA removal was performed applying the Stranded total RNA with RiboZero Plus protocol (Illumina, California, USA, Cat. No.: 20037135). ssRNA-seq (stranded RNA-seq)