GSM6499457: PBMC, CTRL_7; Homo sapiens; RNA-Seq
PBMCs were treated with TRIzol reagent (Invitrogen, Carlsbad, California, USA) and stored at –80°C (5 × 10^6 cells/mL). RNA purity was tested using NanoPhotometer® (IMPLEN, CA, USA). RNA concentration was measured using Qubit® RNA Assay Kit on a Qubit® 2.0 Fluorometer (Life Technologies, CA, USA). RNA integrity was assessed using RNA Nano 6000 Assay Kit on a Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Sequencing libraries were generated using the rRNA-depleted RNA by NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations.