GSM7235372: P05 adult male, bone marrow, year 1 day-0, replicate 1; Homo sapiens; RNA-Seq
PBMCs were isolated using Vacutainer CPT tubes (BD); the remaining red blood cells were lysed with ammonium chloride lysis buffer (Lonza). Cells were immediately used or cryopreserved in 10% dimethylsulfoxide (DMSO) in FBS. A qualified physician's assistant performed ultrasound guided fine needle aspirate (FNA) of axillary lymph nodes under the supervision of a radiologist. Lymph node dimensions and cortical thickness were measured prior to each FNA. Six passes were made using 25-gauge needles, which were flushed with 3 ml of RPMI 1640 supplemented with 10% FBS and 100 U/ml penicillin/streptomycin, followed by three 1 ml rinses. Red blood cells were lysed with ammonium chloride lysis buffer. Cells were washed twice with PBS supplemented with 2% FBS and 2 mM EDTA and immediately used or cryopreserved in 10% DMSO in FBS.  Libraries were prepared using 10x Genomics' Chromium Next GEM single cell 5' gene expression workflow.