GSM8208498: Liver, maternal circadian disruption, rep1; Mus musculus; Bisulfite-Seq
Genomic DNA was extracted using the MagMAX™ DNA Multi-Sample Kit (ThermoFisher 4413020) on the KingFisher Apex instrument (ThermoFisher 5400930) following manufacturer's instructions. 100 ng of gDNA was digested with TaqaI (NEB R0149) at 65⁰C for 2 h followed by MspI (NEB R0106) at 37⁰C overnight. Following enzymatic digestion, samples were used for library generation using the Ovation RRBS Methyl-Seq System (Tecan 0353-32) following manufacturer's instructions.