GSM8121391: MOZ-TIF2 LSK, WM-1119, H3K23pr, rep 1, ChIP; Mus musculus; ChIP-Seq
Cells were fixed in 1% formaldehyde with 5% S2 cells added as an exogenous spike-in control for 8 minutes at room temperature. Chromatin was sheared using a QSonica Probe Sonicator for 16 cycles with 15 seconds on and 45 seconds off with an amplitude of 40%. Sequencing libraries were generated using the NEBNext Ultra II DNA Library Prep Kit (NEB Cat #E7645S).