GSM8256996: gex; Mus musculus; OTHER
Mice -   DIG permeabilization –  The pooled cell suspension is washed, resuspended in 100ul of cold DIG lysis buffer, and incubated for 5min on ice. 1ml of cold DIG wash buffer is then added to the suspension and spun down for 5min at 500g. The pellet is resuspended in 100ul of 1x Nuclei buffer (2000207, 10X Genomics) supplemented with 1mM DTT (10708984001, Sigma) and 2U/l RNAse inhibitor (3335399001, Roche), at a final concentration of 3,400 cells/ul. The library was prepared following the CG000338_ChromiumNextGEM_Multiome_ATAC_GEX_User_Guide_RevE user guide.The sequencing has been performed on a NovaSeq instrument, HiSeq paired-end 150 bp, 75 million reads.