GSM7519462: Control 1, scRNAseq; Mus musculus; RNA-Seq
Organoids were dissociated into single cells. The single cells were checked for viability and processed through the GemCode Single Cell Platform. An input of 7,000 cells was added to each channel of a chip with a recovery rate of 1,200 cells on average. The cells were then partitioned into Gel Beads in Emulsion (GEMs) in the GemCode instrument, where cell lysis and barcoded reverse transcription of RNA occurred, followed by amplification, shearing and 5′ adaptor and sample index attachment.