GSM8292392: S119 with asparagine replicate-4; Streptococcus pyogenes; RNA-Seq
RNA was harvested according to the protocol by Livak and Schmittgen, 2001. RNA purification was conducted using the Direct-zol RNA miniprep kit (Zymo Research). A total of 1 µg of RNA was used to construct sequencing libraries. RNA quality was determined by Agilent 2200 TapeStation analysis. For mRNA library preparation: KAPA Stranded mRNA-Seq Kit was used (Kapa Biosystems, KK8421)