GSM8291797: ZFP740_KO1,replicate1,RNAseq; Homo sapiens; RNA-Seq
Total RNA was isolated from the HepG2 cells using the RNeasy mini kit (Qiagen GMBH, Hilden, Germany) according to the manufacturer's instructions, yielding 150 μg of total RNA. The amount of total RNA was determined using a NanoDrop 1000 spectrophotometer (NanoDrop, Wilmington, DE). The quality of the RNA preparation was evaluated using the Agilent RNA 6000 Nano Kit on an Agilent Bioanalyzer 2100 system (Agilent, Palo Alto, CA). The quality of the preparation was considered to be high based on an RIN value of 8.7.