GSM8121001: wild-type FBS HFSCs 2, ATAC-seq; Mus musculus; ATAC-seq
To resuspend HFSCs, 3D cultures were washed with sterile PBS, dissociated into single cell suspensions using TrpLE-Express (Gibco) for 15min at 37°C. After filtering, cells were pelleted, lysed, and processed as described in Buenrostro et al., 2013. Libraries were prepared according to the manufacturer's guidelines (Illumina). Briefly cells were transposed with Tn5 transposase for 30 min and samples were barcoded.