GSM6756299: CAPN3-point-mutation , no muscle injury 2; Mus musculus; RNA-Seq
RNA was harvested using Rneasy mini plus kit (Qiagen). 1.3 ug of total RNA was used for the construction of sequencing libraries. NEB ordinary library: using fragmented mRNA as a template and random oligonucleotides as primers, the first strand of cDNA was synthesized in the M-MuLV reverse transcriptase system, and then the RNA strand was degraded with RNaseH, and the second cDNA strand was synthesized under the DNA polymerase I system using dNTPs as raw materials. The purified double-stranded cDNA was repaired at the end, A-tailed and ligated to the sequencing linker, and about 250-300 bp of cDNA was screened with AMPure XP beads, PCR amplification was performed, and the PCR products were purified again using AMPure XP beads, and finally the library was obtained. The kit for library construction is NEBNext® Ultra™ RNA Library Prep Kit for Illumina®.