GSM8257071: liver, WT, ZT22, rep2; Mus musculus; ATAC-seq
For the wild type mice biological replicates was merged in the circadian setup, and the tagmentation was performed in three replicates. In the SWI/SNF perturbation models tagmentation was performed on four biological replicates. A 2mL douncer was used to homogenize 15mg of tissue. 2.5 µL of TDE1 enzyme (Illumina 20034197) and 50000 nuclei were used for transposition reactions .  DNA was size selected using AMPure beads (Beckman Coulter A63881) before sequencing. The Illumina Nextera DNA Flex Library Prep Kit was used to prepare libraries for Illumina sequencing (Illumina, 20018705).