GSM7519872: B5234_hom_heart_KO_CaptureHiC_rep1; Mus musculus; Hi-C
We used the Arima HiC kit to process mouse testis and heart tissues. Briefly, frozen tissue was lysed and conditioned before chromatin digestion. The digested chromatin was then filled in and biotinylated before ligation. Next, chromatin was protein-digested and reverse crosslinking overnight, followed by purification. The purified DNA was then sonicated using the bioruptor pico (Diagenode) and size selected before library preparation using the NEB DNA Ultra II, following Arima's protocol. The Hi-C DNA was bound to streptavidin beads before enzymatic end prep, adaptor ligation, DNA release by heat incubation, and lastly, PCR to barcode and amplify the libraries. Capture Hi-C libraries were generated by using the Agilent SureSelect Target Enrichment Kit. capture Hi-C