GSM8072112: P60_GM_scRNA-seq; Mus musculus; RNA-Seq
For single-cell isolation of the early postnatal (P2, P7, and P15) and adult (P60) cortex, thick coronal sections, spanning the rostral end of the lateral ventricles to the rostral end of the hippocampus, were generated from each brain. For whole cortex datasets (P2 cortex scRNA-seq data, P7 cortex scRNA-seq data, P15 cortex scRNA-seq data, P60 cortex scRNA-seq data) the region dorsal to the lateral ventricles was dissected out. This region contained the ventricular-subventricular zone (V-SVZ), cortical white matter, and cortical grey matter. For grey matter datasets (P60 grey matter scRNA-seq data), coronal sections were taken as described above and the region dorsal to the cortical white matter was dissected out. For white matter datasets (P60 white matter scRNA-seq data), coronal sections were taken as described above and the region immediately dorsal to the lateral ventricles, containing both the corpus callosum and V-SVZ, was dissected out. For single cell isolation of the early postnatal V-SVZ (P7 SVZ scRNA-seq) the dorsal and lateral V-SVZ were microdissected out. Libraries were prepared according to the manufacturer's instructions (10X Genomics) using the Chromium Single Cell 3' Library and Gel Bead Kit.