GSM7157944: A549, TRIM8; Homo sapiens; RNA-Seq
RNA quantification was checked using SimpliNano™-Biochrom Spectrophotometers (Biochrom, MA, USA). Sequencing libraries were generated using KAPA mRNA HyperPrep Kit (KAPA Biosystems, Roche, Basel, Switzerland). Synthesis of first strand cDNA using random hexamer primers. After generating double-stranded cDNA (dscDNA), in order to preferentially select cDNA fragments with a length of 300-400 bp, the library fragments were purified with KAPA Pure Beads system (KAPA Biosystems, Roche, Basel, Switzerland).