GSM8032933: VORG4N4_S60; Mus musculus; RNA-Seq
RNA was extracted from tumor cell pellets and cell lines using an RNeasy Plus mini kit (Qiagen, Hilden, Germany). RNA quality was assessed with Nanodrop and BioAnalyzer tools (Agilent, St. Clara, CA). Universal Plus Total RNA-Seq with NuQuant kit (Tecan Genomics, Redwood City, CA) has been used for library preparation following the manufacturer's instructions (library type: fr-secondstrand). RNA samples were quantified and quality tested by Agilent 2100 Bioanalyzer RNA assay (Agilent technologies, Santa Clara, CA) or Caliper (PerkinElmer, Waltham, MA). Final libraries were checked with both Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA) and Agilent Bioanalyzer DNA assay or Caliper (PerkinElmer, Waltham, MA). Libraries were then prepared for sequencing and sequenced on paired-end 150 bp mode on NovaSeq6000 (Illumina, San Diego, CA).