GSM7445631: MSB82; Homo sapiens; RNA-Seq
Sample metadata, including tumor purity, was determined by board-certified pathologists. Samples were required to meet a tumor cellularity as a ratio of tumor to normal nuclei of 20%. Macrodissection was utilized as needed to enrich specimens below the 20% threshold. Solid tumor total nucleic acid was extracted from the FFPE tissue sections using Chemagic 360 sample-specific extraction kits (Perkin Elmer) and digested by proteinase K. RNA was purified from the total nucleic acid by DNase-I digestion. Tempus xT RNA-seq protocol for FFPE solid tumors using transcriptome-capture probes.