GSM7157952: Naïve, ON, rep2; Mus musculus; ATAC-seq
Spleens were harvested from WT and RBPJind mice and RBPJind INT cells (CD3+CD4+CD25-CD45RBint) and WT MNT cells (CD3+CD4+CD25-CD45RBhi) were FACS sorted. Cells were resuspended in transposition reaction mix (25 μl TD, 2.5 μl TDE1, and 22.5 μl Nuclease Free Water) followed by 20 min incubation at 37˚C degrees. Qiagen MinElute PCR Purification Kit was used to purify transposed DNA and DNA samples were sent to Princess Margaret Genomics Centre for downstream PCR amplification and sequencing.