GSM8332683: ATAC_DE_NOD_AC576_20k40k; Mus musculus; ATAC-seq
Briefly, cells were dissociated into single cells, filtered through a Flowmi cell strainer and the nuclei were isolated by incubation with lysis buffer [10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% Tween-20, 0.1% NP-40, 0.01% digitonin and 1% bovine serum albumin (BSA)] for 5 min at 4°C; 20,000 and 40,000 nuclei were isolated. Two replicates of 20k and 40k nuclei were collected per biological sample. Libraries were prepared for sequencing using standard Illumina protocols