GSM8121371: Mixed stage total nuclei, replicate 2; Caenorhabditis elegans; ATAC-seq
Worms were washed 3X with M9 and dounced in ChIP Buffer A for 10 strokes usng a steel dounce. Nuclei suspension was spun down for 1 minute at 100g, and passed through a 5mL cell filter tube. Nuclei morphology was assessed by DAPI, and subjected to flow cytometry for sorting. Tn5 tagmented DNA was amplified as previously described (Buenrostro et al., 2015), using ATAC optimized primers. Amplification was monitored using qPCR, and additional cycles of library amplication was performed as needed. DNA was analyzed on a Bioanalyzer and submitted for Illumina HiSeq.