GSM7961995: PC9_CT_24h_2; Homo sapiens; RNA-Seq
RNA extraction was performed using AllPrep DNA/RNA Mini kit (Qiagen, #80204) according to the manufacturer's protocol. RNA quality was assessed using Fragment Analyzer (Agilent technologies) and the RQN values were provided to confirm the integrity of total RNA. RNA concentration was determined by fluorescent method using Quant-iT™ RNA Assay Kit, Broad Range (ThermoFisher Scientific). RNA samples were processed with Illumina TruSeq® Stranded mRNA Library Preparation Kit following the manufacturer's protocol. Library size and quality were confirmed on Fragment analyzer (Agilent Technologies). KAPA quantification kit for Illumina platforms (KAPA Biosystems, Roche) was used to quantify the library by qPCR. Indexed libraries were pooled and sequenced on an Illumina NextSeq 550 (2x75 bp paired-end reads).