GSM8072021: tdTom pos cells, KO3; Mus musculus; RNA-Seq
The esophageal muscle layer and submucosa were removed mechanically and after enzymatic treatment. The epithelium was minced and incubated in DNaseI and LiberaseTM. After incubation, the cells were filtered and stained with CD324-PE/Cy7  and CD104-A647. Using FACS, the progenitor cells (CD324+CD104+) were isolated based on tdTomato reporter expression. RNA of 2000 cells per sample was isolated and libraries prepared according to Picelli et al. (Picelli, Simone, et al. "Smart-seq2 for sensitive full-length transcriptome profiling in single cells." Nature methods 10.11 (2013): 1096-1098).