GSM7964398: Hyp2_RUVBL2_ChIP; Mus musculus; ChIP-Seq
The hypothalamus was isolated from 8-week-old male C57BL/J mice，cross-linked with 1% formaldehyde for 10 min at room temperature, and then quenched with 125 mM glycine. The chromatin fragments were pre-cleared and then immunoprecipitated with Protein A + G Magnetic beads coupled with anti-RUVBL2 antibody. After reverse crosslinking, CHIP and input  DNA fragments were end-repaired and A-tailed using the NEBNext End Repair/dA-Tailing Module (E7442, NEB) followed by adaptor ligation with the NEBNext Ultra Ligation Module (E7445, NEB). The DNA libraries were amplified for 15 cycles and sequenced using Illumina Hi-Seq with paired-end 2x150 as the sequencing mode.