GSM7864726: Iz_B_Gexp; Mus musculus; RNA-Seq
Freshly isolated splenocytes are treated with RBC lysis buffer to remove red blood cells. Cells from each mouse are hashed with hashing antibodies and stained with anti-B220 and anti-GL7 antibodies and fluorochrome-labelled NP antigen. B220+GL7+NP+ cells were sorted with FACSAria cell sorter. Library preparation was performed according to the manufacturer's instructions (Next GEM Single Cell 5'GEM v3.1 protocol).