NextSeq 500 sequencing
1 �g of total RNA was used as input for ribosomal RNA depletion employing the PAN-Archaea riboPOOL kit (siTOOLS), followed by purification of the depleted RNA with Zymo RNA Clean and concentrator-5 kit (Zymo Research). Sequencing libraries were prepared with the TruSeq Stranded Total RNA Library Kit (Illumina) in combination with the RNA Unique Dual Indices (IDT for Illumina) according to manufacturer�s recommendations. Library enrichment PCR proceeded for 9 cycles. Read-length: 75nt