GSM7159077: A32B-WT-ATAC-2; Homo sapiens; ATAC-seq
hESCs were lysed by lysis buffer (10 mM NaCl, 3 mM MgCl2, 10 mM Tris pH 7.4, and 0.1% NP-40) and the nuclei were resuspended in transposition reaction mix (50% DMF, 50 mM Tris pH 7.5, 20 ng/μl Tn5 transposome and 250 mM MgCl2) and incubated at 55 °C for 10 min. We used transposed DNA, i5 primer, i7 primer and 2X HiFi polymerase mix to amplify library. PCR reaction was incubated at 72 °C for 5 min, and then 98 °C for 30 s, 14 cycles of 98 °C for 15 s and 60 °C for 30 s, followed by 72 °C for 5 min.