GSM8331149: scRNA-Seq - LSK - aged + RhoA-inhibitor (Ri) 2; Mus musculus; RNA-Seq
Cells were concentrated to 300-1000 cells/µl. Cell concentration and viability were determined using a TC20™ Automated Cell Counter (Bio-Rad Laboratories, S.A) upon staining the cells with Trypan blue. Cells were partitioned into Gel Bead-In-Emulsions (GEMs) by using the Chromium Controller system (10X Genomics), with a target recovery of 5000 total cells per sample. cDNA sequencing libraries were prepared using the Next GEM Single Cell 3' Reagent Kits v3.1 (10X Genomics, PN-1000268), following manufacturer's instructions. Briefly, after GEM-RT clean up, cDNA was amplified during 13 cycles and cDNA quality control and quantification were performed on an Agilent Bioanalyzer High Sensitivity chip (Agilent Technologies). cDNA libraries were indexed by PCR using the PN-1000215 Dual Index Kit TT, Set A plate. Size distribution and concentration of 3' cDNA libraries were verified on an Agilent Bioanalyzer High Sensitivity chip (Agilent Technologies).