GSM6961503: SEC-seq for VEGF-A on Hypoxic MSC-loaded Nanovials, mRNA; Homo sapiens; RNA-Seq
Nanovials were loaded directly into the cell mix at a concentration of 500 nanovials per ul for use in the 10x Chromium workflow. Libraries were constructed as described in the Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry Dual Index) with Feature Barcoding technology for Cell Surface Protein and Cell Multiplexing) scRNAseq libraries were constructed as typically described in the guide above. SEC-seq libraries proceeded via normal workflow, with inclusion of construction of a library to detect the oligo-barcoded antibody using the Feature Barcode library workflow in the Single Cell User guide above