GSM6921850: A2058 8q-loss c2 rep 2; Homo sapiens; RNA-Seq
Qiagen RNEeasy In brief, mRNA was purified from total cellular RNA using poly-T oligo-attached magnetic beads. After fragmentation, first strand cDNA was synthesized using random hexamer primers, followed by second strand cDNA synthesis. Subsequently, libraries were prepared with end repair, A-tailing, adapter ligation, size selection, amplification, and purification.