GSM8072173: CD19.28Z_NGFR_OE_TCF7_KO_Donor_TMP502; Homo sapiens; RNA-Seq
Pellets were thawed on ice and processed using either a RNEasy Plus Mini Kit or an AllPrep DNA/RNA Micro Kit (for simultaneous DNA and RNA isolation) (Qiagen) according to the manufacturer's instructions. Total RNA was quantified using either a Qubit Fluorometer or a DeNovix DS-11 FX Spectrophotometer/Fluorometer Sequencing was outsourced to Novogene - US. Libraries were prepared by the vendor and sequenced on a NovoSeq 6000 instrument.