GSM7037179: tumor-bearing liver, vehicle treated; Mus musculus; RNA-Seq
mouse livers were minced, digested and filtered for single cell sequencing About 8-10K single cells with viability score of >70% were input in each library. The 10x Genomics Chromium controller instrument was used for Gel Bead- in Emulsion (GEMs) preparation. Chromium Next GEM Single Cell 3' Kit v3.1 (PC-1000269), Chromium Next GEM Chip G Single Cell Kit (PC-1000127) and Dual Index Kit TT Set A Kit (PC-1000215) were used for Single-Cell Library Preparation. cDNA and final barcoded sequencing libraries were generated as per the manufacturer's specifications and their quality and concentration was assessed using the Bioanalyzer 2100 and qPCR, respectively. Quality pass single cell libraries were sequenced on NextSeq550 sequencer using the paired-end 75bp sequencing kit.