GSM7622475: Veh1; Mus musculus; RNA-Seq
For RNA sequencing, cells were washed and resuspended in RLT Lysis Buffer plus beta-mercaptoethanol (10 μL per 1 mL of buffer, Sigma, Catalog# M6250). RNA was extracted using the RNeasy Plus Micro Kit (Qiagen) according to manufacturer instructions with a genomic DNA elimination column step. We utilized the Nugen Universal Plus mRNAseq (input >10ng) for library preparation. RNA-seq: we sequenced 20 million 100bp paired end reads per sample with three biological  replicates in three conditions (Homogenate + Vehicle, Homogenate + Torin-2, Homogenate + Rapamycin).