GSM6533776: Pan02 whole tumor, Arg2-derived peptide vaccine, mouse 1 [Tumor_5]; Mus musculus; RNA-Seq
Tumor fragments (≤ 20 mg) in RNAlater (Invitrogen) were transferred to RLT buffer (Qiagen) and mechanically homogenized with a Tissue Lyser (Qiagen). RNA was extracted with the RNEasy Plus Mini Kit (Qiagen), 500ng purified RNA (RIN score > 7) was enriched for polyadenylated mRNA followed by fragmentation. Random primmed cDNA synthesis was performed using NEBNext kits. This was followed by hairpin adaptor ligation and PCR mediated indexing (NEBNext). The library was size selected using AMPure beads and quantified by KAPA (Roche).