GSM7734694: Prism, C&T K27Ac; Paracentrotus lividus; OTHER
Cut-and-Tag: The assay was performed using the CUT&Tag-IT™ Kit (Active Motif) and H3K27ac antibody (Active Motif Ref 39135)). Cut-and-Tag: Following tagmentation, beads were magnetized, washed, and incubated for 1h at 37°C with proteinase K and SDS to digest the chromatin and release DNA fragments. At the end of the incubation, beads were magnetized and DNA was purified using 625 µl DNA binding buffer. Following purification, libraries were amplified by using 25 µl of DNA sample and 2.5 µl of a uniquely barcoded i5 primer and 2.5 µl of a uniquely barcoded i7 primer in a 50 µl PCR reaction with Q5 high-fidelity DNA polymerase.