Illumina NovaSeq 6000 paired end sequencing; ChIPseq - The ETO2 scaffolding factor maintains acute leukemia by driving a MYB/EP300-dependent oncogenic program
After growth, cell was wash in PBS and then fixed in 2mM DSG (incubation 45min at RT). Cell was washed in PBS and then incubated in PBS supplemented with 1% PFA for 10min. Reaction was quenched by the addition of 0.125M (final concentration) of Glycine After cell lysis, nuclei was digested (by Micrococcal nuclease) and/or sonicated. Sheared chromatin was then used for immunoprecipitation. DNA fragments were amplified by PCR with indexed paired-end adaptors (Illumina).