GSM8254904: THP1, UL135stopvirus, undifferentiated, day3, rep3; Homo sapiens; RNA-Seq
Total RNA was extracted with the Zymo Duet Quick-DNA/RNA Miniprep kit. Manufacturer protocols were followed for column isolation of total RNA, DNAse treatment, and RNA cleaning and concentration. cDNA libraries were prepared with an Illumina TruSeq Stranded mRNA kit and a KAPA Dual-Indexed Adaper kit. The NGS library was quantified with a qPCR-based KAPA Library Quantification kit. Manufacturer protocols were followed.