GSM7244859: RNA-seq_WT_R3; Campylobacter jejuni; RNA-Seq
total RNA was treated with DNase I to digest genomic DNA and ribosomal RNA was depleted adapter ligation protocol: After ultrasound fragmentation, an adapter was ligated to RNA 3' ends and reverse transcription was performed, followed by ligation of 5' Illumina TruSeq sequencing adapters to the 3' ends of the antisense cDNAs. The resulting cDNA was PCR-amplified, purified with the Agencourt AMPure XP kit (Beckman Coulter Genomics), and analyzed by capillary electrophoresis.