GSM7244786: HA843, oligodendrocyte co-cultured with T cells separated by insert (TCM), scRNAseq; Homo sapiens; RNA-Seq
In brief, media was collected and cells were treated with PBS-EDTA to detach the oligodendrocytes from the bottom of the well. Once cells were visibly detached, the suspension was combined with the collected media and the wells were washed twice with PBS to collect any residual cells and transferred to the same sample tube. The samples were centrifuged at 800rpm for 10min to pellet the live cells and then resuspended in PBS at a concentration of 350 thousand cells per milliliter. Approximately 10,000 cells were used for library construction. Library was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics).