GSM8332114: D. melanogaster vigEP812 -/-, testis; Drosophila melanogaster; RNA-Seq
Total RNA for RNA-seq libraries were isolated from vasEP812-/-, vasEP812+/-, and vasEP812-/-; rhi-GFP D. melanogaster gonads with ExtractRNA (Evrogen). RNA was depleted with Dynabeads MyOne Streptavidin C1 (Thermo Scientific Fisher) conjugated with synthesized anti-rDNA oligos. RNA-seq libraries were generated using the NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina (#E7760, NEB) and NEBNext Multiplex Oligos for Illumina (Index Primers Set 1, NEB #E7335, and Set 2, NEB #E7500) according to the manufacturer's instructions and sequenced on the Illumina NovaSeq 6000 SP platform (100 bp runs).