GSM7794577: eIF4E_IPed_RNA_4EBP1_4A_replica1; Homo sapiens; RIP-Seq
Total RNA was extracted with Qiagene Rneasy Kit. Ribosomal footprints were extracted from the monosomal fraction after RNaseI digestion via the size selection. eIF4E-IPed RNA was extracted by acid phenol extraction with the protocol previously described (PMID:32589966) Libraries from total and eIF4E-IPed RNA were prepared using the Illumina TruSeq Stranded library preparation kit. Ribosomal footprints were converted into libraries with the use of Next-Flex small RNA v.4 kit protocol