GSM8120514: HPAEC, 5dyne 60mmHg, rep 2; Homo sapiens; RNA-Seq
RNA was isolated by perfusion of channels with lysis buffer and RNA purified using Qiagen RNeasy micro kits RNA quality was evaluated using an Agilent Tapestation where all samples had RINe scores ≥ 9.7. Libraries were prepared from RNA samples using SMART-Seq v4 Ultra Low Input (Takara) and Nextera XT DNA Library Preparation (Illumina) kits.