GSM7243465: HSALR, vehicle 2; Mus musculus; RNA-Seq
Total RNA was extracted from gastrocnemius of mice using Trizol. RNA integrity was examined by agarose gel electrophoresis.Extracted RNA was usedfor RNAseq analysis in Omega Bio services (Norcross, GA, USA).RNA samples were re-purified usinga Qiagen RNeasy cleanup kit.The quality of the isolated RNA was verified by calculation of RNA integrity number using Agilent Technologies.Ribosomal RNAs were depleted from the RNA samples prior to libraries preparation. Libraries were sequenced with at least 60M reads per sample