GSM6717596: EDN MSN01-03R-CM Rep 3 (Conv); Homo sapiens; RNA-Seq
Total RNA was isolated using Trizol (see DToxS SOP A – 1.0: Total RNA Isolation) Library was prepared using 300 ng of total RNA enriched for mRNA using polyA beads and the TruSeq RNA Library Prep Kit (Illumina, Cat: RS-122-2001) per manufacturer's instructions (See DToxS SOP A - 6.0: High-throughput mRNA Seq Library Construction for 3' Digital Gene Expression (DGE)