GSM7444229: Wild-type mouse liver; Mus musculus; miRNA-Seq
Total RNA of murine liver was prepared using the miRNeasy Mini Kit (Qiagen), according to standard manufacturer's protocol. Eliminate DNA contamination using DNase. The small RNA libraries were prepared using SMARTer smRNA-seq kit (Takara Bio.). The single end fragment library (50 bp) was sequenced with the HiSeq2500. The resulting reads were then mapped to the mm10 build of the mouse genome and to miRBase (v22.1) sequence. Read sequences were classified according to the information registered in miRBase to determine whether they were derived from 5p or 3p of Pre-miRNA.