RNA sequencing of the red inner leaves from a  double haploid line Y007-P-24 in ornamental kale
Y007-P-24 is a red leaf double haploid line from the Osaka Red variety (TAKII, Japan). The plants exhibited clear coloration with new red leaves during their decorative period when they were grown in a greenhouse at Shenyang Agricultural University (Shenyang, China). Leaf samples were harvested at three developmental stages (Figure 1b): S0 (white leaf), S1 (pink leaf and white main vein), and S2 (red and slightly green leaf and white main vein). New red leaves first appeared at the end of September to early October when the temperature decrease below 15 C. The phenotype appeared from the end of September to March of the following year. At S0S2, samples of whole leaves without their main veins were collected, and three independent biological replicates were used. All samples were immediately frozen in liquid nitrogen and stored at -80 C for pigment measurement, RNA-seq, and qRT-PCR.