whole genome sequencing of Aedes aegypti: adult whole body
We extracted genomic DNA from individual wild-caught adult Aedes in Colombia using Genetra Puregene Tissue Kits (Qiagen, Valencia, CA, USA), constructed barcoded libraries for sequencing using KAPA HyperPrep kits (Roche Sequencing, Pleasanton, CA) with a target fragment size of 500 bp. We assessed the size of the library inserts using a Tapestation 4150 (Agilent, Santa Clara, CA) and dsDNA screentape (Agilent, #5067-5365).