long read adaptive sampling
For each participant, 1ug of high molecular weight DNA was sheared to 10-12kb through a gTUBE (Covaris, Woburn, MA). Fragments were then end repaired, A-tailed, and ligated to barcoded adapters using the native barcoding kit SQK-NBD114.24. Four barcoded libraries were pooled and 50fmol loaded onto R10 PromethION Flow Cells and sequenced for 72h in adaptive sampling mode enriching for a 10MB FASTA reference.