Biological replicate 2 of Vd2190
Total RNA was extracted and purified with the RNAprep Pure Plant Kit (Tiangen Biotech, Beijing, China) from each sample. The poly(A)+ mRNAs from other samples were enriched using oligo (dT) magnetic beads. Quality of RNA was evaluated by agarose gel electrophoresis and BioAnalyzer-2100 (Agilent). Strand-specific RNA-Seq libraries were prepared with the NEBNext Ultra Directional RNA Library Prep Kit following the manufacturer's instruction, and sequenced using the Illumina HiSeq 2500 system with the 2*150 bp paired-end read mode