16s rDNA of Chinese bamboo rat: adult male feces
Microbial genomic DNA was extracted from each fecal sample (0.2 g) using CTAB/SDS method. The V4 region of 16S rRNA genes was amplified used the specific primers 515F 5-CCTAYGGGRBGCASCAG-3 and 806R 5-GGACTACNNGGGTATCTAAT-3 with the barcode. After PCR products quantification, qualification, mixing and purification, sequencing libraries were generated using TruSeq DNA PCR-Free Sample Preparation Kit (Illumina, USA) following manufacturer's recommendations. The library quality was assessed on the Qubit@ 2.0 Fluorometer (Thermo Scientific) and Agilent Bioanalyzer 2100 system. At last, the library was sequenced on an IlluminaHiSeq6000 platform and 250 bp paired-end reads were generated.