FACS-sorted NHBE secretory cells, 12hrs post infection with Cal/04/09, replicate 2 of 3
After infection with IAV for 12 hours with Cal/07/09, cells were sorted into secretory and ciliated cells using FACS, RNA extracted using RNeasy Plus Micro Kit, and cDNA libraries were prepared with the Clontech SMARTer PicoMammalian kit.