ITS2 sequencing of coral-associated Symbiodiniaceae
ITS2 (Symbiodiniaceae) amplicon-based sequencing. ITS2 primers SYM_VAR_5.8S2 (5-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGGA ATTGCAGAACTCCGTGAACC-3) and SYM_VAR_REV (5-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGCGGGTTCWCTTGTYTGACTTCATGC-3) (Illumina adaptors underlined) were used in a first-round PCR to amplify a ~450 bp fragment of the ribosomal ITS-2 region.