RNA-seq of Bacilus substilis Mutant-type Time 1 Rep 1
Total RNA was extracted and purified using the Tiangen RNAprep purification kit. RNA is quantified using the Turner TBS-380 fluorometer. rRNA was removed using Hieff NGS MagSP rRNA Removal Kit. The illumina library was prepared with Hieff NGS Library Prep Kit using fragmented RNA before sequencing.