GBS black dry beans
DNA was extracted from trifoliate leaves using NucleoSpin Plant II Kit  following the Genomic DNA from plant protocol. DNA concentration was measured by using Quant-iTTM PicoGreenTM dsDNA Assay Kit, and 10 ng/ul of DNA was used for GBS library preparation according to Elshire et al. 2011 with a single restriction enzyme, ApeKI. Each plate of 96-wells was sequenced in a lane of an Illumina HiSeq platform using single-end reads at RTSF Genomics Core at Michigan State University.