CO1 metabarcoding of eDNA
Segments of the cytochrome oxidase I gene (COI) were amplified in triplicate using the forward primer mlCOIintF (Leray et al. 2013) and reverse primer HCO2198 (Geller et al., 2013) with part of the Illumina (San Diego, CA, USA) adapters 5-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG3 (forward) and 5-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG3 (reverse) attached in a two-step untagged library preparation.