RNA-Seq to evaluate kinetics of changes to transcriptome of Acanthamoeba ATCC 30461 exposed to contact lens materials.
Samples were DNAse treated with Invitrogen DNAse (RNAse free). Library preparation was performed using Illuminas Stranded Total RNA Prep Ligation with Ribo-Zero Plus kit and 10bp IDT for Illumina indices. Sequencing was done on a NextSeq2000 giving 2x50bp reads.