RNA-Seq of mus musculus: adult male heart (Fe diet + amlodipine)
RNAseq libraries were constructed from 500 ng of total RNA using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB). Polyadenylated mRNAs were enriched with oligo dTs conjugated to paramagnetic beads. Enriched mRNAs were fragmented