RNA-Seq of Gryllus longicercus: male thoracic ganglia replacement sample 1 (T1+T2)
Insect tissues come from virgin adult males and females 7-10 days after molting from the same source population as the reference genome. Crickets were cold anesthetized and then dissected submerged under RnaLater and sent to Azenta. Total RNA was extracted from tissues in RNALater samples using Qiagen RNeasy Plus Universal micro kit, quantified using Qubit 3.0 Fluorometer and its integrity was checked using Agilent TapeStation 4200. Libraries were prepared with NEBNext Ultra II RNA Library Prep. The sequencing libraries were validated on the Agilent TapeStation and quantified using Qubit 3.0 Fluorometer as well as by quantitative PCR. The sequencing libraries were clustered on a lane of a flowcell which was later loaded on the Illumina HiSeq instrument. The samples were sequenced using a 2x150bp Paired End configuration. Raw sequence data was converted into fastq files and de-multiplexed using Illumina's bcl2fastq 2.17. One mismatch was allowed for index sequence identification.