RNAseq of Drophila heads
Total RNA was extracted from 100L fly heads (male and female) using TRIzol (Invitrogen) and the manufacturers recommended protocol. RNA samples were subjected to quality control before and after the cDNA libraries were prepared using TruSeq Stranded mRNA Library Prep Kit from Illumina. cDNA samples were normalized, pooled, then run using a P2 flowcell on an Illumina NextSeq 2000.