PacBio Seq of Ranunculus cassubicifolius
We followed the manufacturers instructions for library prep. Sequencing (HiFi CCS) was performed using the Pacific Biosciences Revio instrument (24 h movie time, loading concentration 230 pM, 2 h pre-extension time, diffusion loading, mean insert length according to SMRT link raw data report: 18.0 kb) following standard manufacturer's protocols.Please see the details in the upcoming article on Ranunculus cassubicifolius genome sequencing.