RAD-seq of G.aculeatus
Restriction site association DNA sequencing (RAD-seq) was performed using the PstI enzyme and a P1 adaptor with unique barcodes. Forward amplification and sequencing primers were ligated. Ligation products were randomly sheared, and size selected using a 300-500bp window. P2 adaptors were ligated and fragments with P1 and P2 were selectively amplified by PCR. The barcoded RAD libraries were sequenced using paired-end strategy (150 PE sequencing) on an Illumina HiSeq2000 platform