RNAseq of SCLC PDX: MGH1572-4A
Frozen tissue fragments were lysed and homogenized with the TissueLyser (Qiagen) and RNA was extracted with the RNeasy Mini Kit (Qiagen). RNA quality was assessed with 1% agarose gel electrophoresis, Nano-drop for RNA amount and purity, and Agilent2100 for RNA integrity number. Library preparation at Novogene with Poly-A enrichment (250-300 bp insert cDNA library). Samples with an RNA integrity number (RIN) of > 8 were selected for library construction.