RNA-Seq of Solanum lycopersicum pollen tube: Nagcarlang, 28C, 120 minutes, replicate 1
For pollen grains, thin, plastic pestles were used to grind up the plant material in a 0.5 ml Eppendorf tube on a frozen tube block for 1-2 minutes. After grinding, the eppendorf tubes (with sample and pestles still inside) were moved to wet ice and 450 l of buffer RLT with 1% -ME was added to each tube. The pestle was carefully removed after ensuring all plant material was washed off. Then the RNA extraction process was continued according to the Qiagen RNeasy protocol. RNA was quantified by nanodrop. Additional quality control was performed by Genewiz/Azenta before library preparation.