Prop_Crtl_I 2
100 ng of total RNA from each sample was used for preparing the libraries with the NEBNext Ultra II Directional RNA Library Prep kit (New England Biolabs, #E7760), using the rRNA depletion method. Libraries were validated with TapeStation and all samples were pooled and sequenced using the NextSeq 500 System (Illumina) in runs of 2x75 cycles, yielding at least 30 million reads per sample.