Rna-Seq of skin bacterial communities in N. perstriatus
We performed bacterial community sequencing of DNA extracts, targeting the V4 region of the bacterial 16S rRNA gene and amplified via PCR with 515F and 806R primers (Caporaso et al. 2012). The resulting amplicon products were pooled and purified using AMPure XP beads (Beckmann-Coulter) and sequenced on the Illumina Miseq platform (v2 Micro kit) at the University of Floridas Interdisciplinary Center for Biotechnology Research (Gainesville, FL, USA), generating 2 x 150 base-paired end reads.