RNAseq for AD:day51
DNA extraction was performed using the E.Z.N.A. soil DNA Kit (Omega Bio-tek, Norcross, GA, U.S.). Modified 515F/806R primer pair was used for amplification. The PCR product was extracted using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) and quantified using Quantus Fluorometer (Promega, USA). Purified amplicons were paired-end sequenced on an Illumina MiSeq PE300 platform (Illumina Inc., CA, US). All sequenced sample libraries were quality-filtered by fastp version 0.19.6 and merged by FLASH version 1.2.7.