Metagenomic analysis of plastic debris from the North Atlantic and North Pacific garbage patches
DNA was extracted from the plastic samples using the Macherey Nagel DNA Nucleo spin soil kit (Nucleo Spin TM Soil kit Macherey-Nagel TM, Dren, Germany) according to the manufacturer's instructions. DNA concentration was measured using a nano Qubit (ThermoFisher). The metagenomic sequencing was performed on a NovaSeq 6000 Illumina sequencing platform at LGC Genomics GmbH, Berlin, Germany.