16 rRNA gene seq of Tannourine water
Water samples were filtered (0.22 m pore-size filters) to capture microbial communities and the DNA was extracted from the filters using a MP Bio FastDNA TM Spin Kit. The V3-V4 hypervariable regions of the 16S rRNA gene were amplified using primers 341F and 805R. PCR amplification was performed in duplicate for each sample, and the resulting products were pooled and purified using the AMPure XP beads (Beckman Coulter). Sequencing libraries were prepared using the Nextera XT DNA Library Prep Kit (Illumina) and sequenced on an Illumina MiSeq platform with 2x300 bp paired-end reads.