HiC data of adult male liver from Mus musculus (WT)
The methodology for generating Arima-HiC libraries from fresh frozen livers involves a series of precise steps to capture the spatial interactions within the genome. Initially, the livers were crosslinked and digested using a cocktail of restriction enzymes (RE). The resulting 5-overhangs were then filled in, leading to the labeling of the digested ends with a biotinylated nucleotide. Subsequently, spatially proximal digested ends of DNA were ligated, enabling the capture of both the sequence and structure of the genome. The ligated DNA underwent a purification process, yielding pure proximally-ligated DNA. This DNA was then fragmented using the Bioruptor Pico from Diagenode. Biotinylated fragments were selectively enriched using Streptavidin beads.The enriched fragments underwent a custom library preparation protocol that utilized the Swift Biosciences Accel-NGS 2S Plus DNA Library Kit (Cat # 21024 or 21096) and the Swift Biosciences Indexing Kit (Cat # 26148, 26248, 26396, 26596, 26696, 26796, 26896, or 269384). These kits collectively contributed to the creation of Arima-HiC libraries, which were then prepared for sequencing.