RNA_Nat10_DcKO1
Total RNA was extracted from 7 days testis, DNase I-treated total RNA samples were subjected to polyA mRNA isolation, followedby heat-induced mRNA fragmentation prior to first-strandcDNA reverse transcription using randomized hexamerprimers. After second-strand DNA synthesis, the doublestrand DNA was end-repaired prior to dA-tailing, followedby Y adaptorligation, according to standard protocols