Hela WGS by Illumina
Genomic DNA was isolated from samples using standard methods involving phenol/chloroform extraction and isopropanol precipitation. Sample quality was assessed using a Nanodrop spectrophotometer (Thermo Scientific) and Genomic DNA Screen Tape (Agilent).  We used 500 ng genomic DNA for whole-genome sequencing library construction.  Briefly, the purified genomic DNA was fragmented by Bioruptor (Diagenode), and the library was prepared by KAPA HyperPrep Kit (Roche) following manufacturer's protocol.   Whole-genome sequencing (WGS, 2 × 150-bp paired-end reads) was performed using the NovaSeq6000 sequencer via the Advanced Technology Genomic Core at the University of Texas MD Anderson Cancer Center.