HAP1_Chr12_1Mb-edit4
Genomic DNA of single cell clones were extracted and sheared by Covaris S206 instrument. Libraries were prepared through end repair, A-tail addition, and adapter ligation using VAHTS Universal DNA Library Prep Kit (Vazyme). DNA fragments of the whole genome were amplified using specific primers (Vazyme) and sequenced using illumine platform. The NGS reads were filtered by Fastp with default parameters. BWA was used to align paired reads to reference genomes. Samtools sorted and compressed the alignment result to BAM file. Mosdepth was used to calculate the coverage depth via these bam files in individual window sizes. The graphs were plotted by R.