DNA sequencing faecal matter
Amplified DNA fragments were end-repaired, and A-tailed. The                 sequencing adapters were ligated to the ends of the DNA fragments using DNA-binding                 enzyme, and the DNA fragments were purified using AMpure PB magnetic beads to                 construct a SMRTbell library. Finally, sequencing primer was annealed to the                 SMRTbell templates, followed by binding of the sequence polymerase to the annealed                 templates