RNA-Seq of WT-light-1
After the RNA samples were qualified, magnetic beads with Oligo(dT) were used to enrich mRNA. The mRNA was broken into short fragments, and was used as template to synthesize cDNA. The purified double-stranded cDNA was repaired at the end, A tail was added, and sequencing adaptor was connected. Then AMPure XP beads were used for fragment size selection. PCR amplification was performed and PCR products were purified to obtain the final library.