Shotgun-metagenomic sequencing of Onthophagus taurus larval foregut
Larval foreguts were dissected out of sterilized larvae (n=5), pooled, enriched for bacterial DNA via dounce homogenization and centrifugal fractional, and DNA extracted using bead beating and phenol-chloroform extractions. DNA was then fractionated and size selected to ~300bp before being barcoded using the NEBNext Ultra II protocol. Finally, sample was pooled with other samples and run on a 300 cycle NextSeq 500 sequencer.