white_424_pupa_0h
Non-polyA selection strand-specific mRNA libraries were prepared by using TruSeq Stranded Total RNA Library Prep Gold.Adapter was then ligated to DNA fragments by using T4 DNA ligase (Enzymatics, Cat# L603-HC-L). After UDG (NEB, Cat# M0280S) treatment, DNA fragments were amplified by using Phusion HF DNA polymerase (NEB, Cat# M0530L). Libraries were sent to Novogene for quality control and sequencing.