RNAseq of SCLC PDX: MGH1531-5BX
Frozen tissue fragments were lysed in TRIzol (Invitrogen) and homoginized by rotor, followed by chloroform extraction of total RNA, DNAse treatment (Qiagen) and clean-up using RNeasy mini kit (Qiagen). RNA amount and quality was assessed using Agilent 2100 Bioanalyzer. RNA was processed into cDNA libraries by the Kapa Stranded RNA-seq kit with RiboErase HMR method and was sequenced on NextSeq 500 (18 pooled barcoded samples).