Patient #7 omental white adipose tissue TCR-seq
TCR beta libraries were prepared from RNA with a custom procedure based on multiplex PCR and adapter addition by PCR extension to avoid ligation biases. The libraries were sequenced with the Ion Torrent S5 System using Ion 530 chips (Ion Torrent, Thermo Fisher Scientific). Preprocessing of FASTQ files involved selection of reads of length between 400 and 500 nucleotides (expected amplicon size) and splitting by sample barcode identification. Downstream immune repertoire analyses were performed with RTCR and immunarch R package.