16S_amplicon_sequencing: 16_26 Cockle
The V4 region of the 16S rRNA gene (287 bp long) was amplified from 28 genomic DNA samples using the universal prokaryotic-specific primers 16S rDNA described in Lasa et al. (2019). A first target enrichment PCR assay was performed with the 16S conserved primers, followed by a second PCR assay, with customized primers including adapter complementary regions (Lasa et al., 2019). Libraries obtained were sequenced using an Ion Torrent (PGM) Platform (Thermo Fisher Scientific, MA).