RNA-Seq of nuclei isolated by INTACT from Drosophila expressing vGATvAChT Split-Gal4/UAS-UNC84-GFP
Nuclei were isolated using the INTACT method as described in Jauregui-Lozano et al, 2021. RNA was extracted using Trizol. Poly(A) RNA was selected using the Poly(A)Purist MAG Kit. Libraries were prepared using Nextflex Rapid Directional RNA-Seq Kit.