WGS of SAC1-degron H9 hES cells
DNA quantity of the samples was measured with Quant-iT dsDNA Broad-Range Assay-Kit (Thermo Fisher Scientific) using the Gemini XPS microplate reader (VWR). 100 ng DNA of each sample was used for library preparation with the TruSeq Nano DNA Library Prep Kit (Illumina) according to the manufacturers recommendations. Next generation-sequencing was performed on a NovaSeq 6000 platform (Illumina) with 2 x 150 bp read mode. The generated sequencing data were demultiplexed with Illumina bcl2fastq (2.20). Adapters were trimmed with Skewer (version 0.2.2) (Jiang et al. 2014). Quality trimming of the reads has not been performed. Sequencing data analysis was performed using the Illumina DRAGEN platform (software version 4.2.4).