gRNA screening for ARO4 promoter in Yeast, Group 3
A pool of gRNAs targeting TEF1 promoter was introduced into three yeast strains expressing different activation domains. Approximately 80 clones were randomly picked per activation domain. The clones were grown in 96-deep well culture. Their cell density and red fluorescence were quantified. Every strain had a red fluorescence protein cassette in its genome driven by a CYC1 promoter. Based on the fluorescence level, the cultures are splitted into five groups, with their plasmid are extracted for nanopore sequencing.