Population genetics of Corylus cornuta
Genomic DNA was converted into nextRAD genotyping-by-sequencing libraries. Genomic DNA was first fragmented with Nextera Flex reagent (Illumina, Inc.), which also ligates short adapter sequences to the ends of the fragments. Fragmented DNA was then amplified for 26 cycles at 73 degrees, with one of the primers matching the adapter and extending 9 nucleotides into the genomic DNA with the selective sequence GTGTAGAGC. The nextRAD libraries were sequenced on a Novaseq 6000 with two lanes of 122 bp reads (University of Oregon).