sRNA-Seq of T.gondii: whole cell RNA: enriched mitochondria
200 ng of RNA was used to generate sequencing libraries using the NEBNext Multiplex Small RNA Library Prep Set for Illumina Kit (New England BioLabs) according to manufacturers instructions. For identification of PCR duplicates in the library preparation process the 5-linker was modified with 8 N-nucleotides (as Unique Molecular Identifiers, UMIs).  Small RNA library clean-up was carried out with AMPure XP Beads (Beckmann Coulter).