mitochondrial genome of the triploid hybrid grouper Epinephelus fuscoguttatus female x E. lanceolatus male
we used long and accurate PCR with 19 pairs of primers designed specifically for triploid hybrid grouper Epinephelus fuscoguttatus (female) x E. lanceolatus (male) to amplify the mitochondrial sequences, then the products were sequenced and assembled into the mitochondrial genome.