Single Cell RNA-Seq of Crotalus atrox Motor Neurons - Sample CA4
51 locomotor MNs and 62 shaker MNs were individually collected from spinal cord slices of 6 rattlesnakes, transferred into 96 well plates containing lysis buffer and barcoded oligo-dt primers and stored at -80 until library preparation. Single-cell RNA-seq libraries were prepared following the mcSCRB-seq protocol v 1.140. i7 index N714 was used for the locomotor cell plate, i7 index N715 was used for the shaker cell plate. Resultant libraries were then pooled and run on 0.5 lanes of a v4 high output flowcell, using an Illumina HiSeq 4000 sequencing instrument.