root metagenome from Z. Maize, Treatment: no fertil. | plant beneficial organisms, rep.1
DNA was extracted using the NucleoSpin Soil Kit (Macherey Nagel). Labwork: Petra Lange, Lena Geist, Renate Wolfer.The amplification of DNA was carried out by LGC genomics GmbH (Berlin, Germany) using the primers 515f and 806r for bacterial DNA, ITS1fKyo2 and ITS86r for fungal DNA and AMF and AML2 for mycorrhizal DNA. Amplicons were sequenced on an Illumina MiSeq instrument with 300bp paired-end reads.