Genotyping-by-sequencing of Melaleuca alternifolia: Ma_003 (Upland ecotype)
DNA was extracted from frozen tissue of young leaves using the DNeasy 96 Plant kit. Then, 100 ng of genomic DNA were used for library preparation, with 1.44 ng of total adapters. The genomic DNAs were restricted with PstI enzyme and the library was amplified with 18 PCR cycles