RNA-Seq of mus musculus
Libraries for Nanopore sequencing were prepared according to the manufacturers protocol for direct cDNA Sequencing (SQK-DCS109 Oxford Nanopore Technologies) with the following minor modifications: The protocol was started with 200 ng cDNA with the End-prep step. Final library elution was performed in 15 l to have material left for TapeStation analysis and Qubit BR measurement. Four GridIon flow cells (FLO-MIN106) were loaded with 12 L libraries (74115 ng) by using a Flow Cell Priming kit EXP-FLP002. Base calling was done with Guppy v5.0.12. The High accuracy (HAC) model was selected for base calling (Q-Score cut-off [Math Processing Error] 9).