FACS-sorted NHBE secretory cells, 6hrs post infection with Cal/04/09, replicate 3 of 3
After infection with IAV for 6 hours with Cal/07/09, cells were sorted into secretory and ciliated cells using FACS, RNA extracted using RNeasy Plus Micro Kit, and cDNA libraries were prepared with the Clontech SMARTer PicoMammalian kit.