Metagenome from Brazilian Mangroves sediments
Total DNA extraction was performed using the PowerSoil DNA Isolation Kit (Mobio Labs, Inc., Solana Beach, CA, USA) according to the manufacturers guideline at  SENAI Institute of Innovation in Biosynthetic and Fibers (SENAI CETIQT, Rio de Janeiro, RJ, Brazil). Metagenomic library preparation and sequencing were performed at Computational Genomics Unity Darcy Fontoura de Almeida (UGCDFA) of the National Laboratory of Scientific Computation (LNCC) (Petrpolis, RJ, Brazil). Libraries were constructed using the Nextera DNA Flex library preparation kit (Illumina, USA) according to the manufacturers recommendations. Sequencing was conducted on a Illumina NextSeq 500 platform (Illumina, San Diego, CA, USA) using the NextSeq 500/550 high-output kit v2.5 (Illumina, USA), with the system set to produce 2150-bp reads.