miRNA-Seq of Coptotermes formosanus Shiraki: worker head
RNAs of 18-30nt were enriched by polyacrylamide gel electrophoresis. The libraries were constructed by the NEBNext small RNA library prep set (New England Biolabs; E7300). The libraries were evaluated by Agilent 2100 and ABI StepOnePlus Real-Time PCR System (Life Technologies). Finally, the libraries were sequenced by Illumina Hiseq2000