snRNAseq of control mus musculus pancreatic islets
Approximately 14,000 single nuclei were loaded onto a 10X Genomics Chromium machine and resulting captured cells were sequenced via 10X Genomics Single Cell 3P v3 chemistry (10x Genomics, USA), yielding approximately 7k nuclei per lane. All libraries were analyzed using the Cell Ranger analysis pipeline version 3.0. Briefly, reads were aligned to mouse reference sequence mm10 and unique molecular identifiers (UMIs) were quantified using the Cell Ranger count software with default parameters. Count data from each single nucleus were normalized to the same sequencing depth and then aggregated using the cellranger aggr tool. Cell barcodes with fewer than 1000 detected genes and a total mitochondrial content > 20% (signifying potential apoptotic cells) were discarded from analyses.