Raw sequence reads of Malus Hopa: adult tree
One microgram qualified genomic DNA per sample was randomly fragmented to 300-500 bp using a ultrasonication (Covaris, Woburn, Massachusetts, USA). Then the sequencing libraries were constructed following the procedures provided by the the VAHTS Universal DNA Library Prep Kit for MGI (Vazyme, Nanjing, China). The index codes were added to attribute sequences to each sample. The Library quantification and size was measured using Qubit 3.0 Fluorometer (Life Technologies, Carlsbad, CA, USA) and Bioanalyzer 2100 system (Agilent Technologies, CA, USA). The DNA libraries were subsequently sequenced on a MGI-SEQ 2000 platform by Frasergen Bioinformatics Co., Ltd. (Wuhan, China) to generate 150-bp paired-end reads.