RNA-seq of pig musculus: longissimus dorsi muscle
A pool of equimolar ratios of RNAs from three individuals was established. The pooled RNA samples were purified with an RNeasy Micro Kit (Cat. Qiagen-74004; venlo, The Netherlands) for the preparation of complementary DNA (cDNA) library (approximately 3  of total RNA). Poly (A) messenger RNA (mRNA ) isolation, first and second strand cDNA synthesis, fragment and adaptor ligation, and cDNA library preparation were performed sequentially with the TruSeq RNA Sample Prep Kit (Cat. RS-122-2002; Illumina, San Diego, CA). After that, each sample product was loaded onto the flow cell channels of the HighSeq 2000 platform (Illumina) for double-end 150 bp sequencing. The insert size of the double-ended library is 380 bp, and three biological replicas were included in each group.