FMDV S fragment mutants (proximal)
Total RNA extraction, Reverse transcription using random hexamers and two FMDV-specific primers, second strand synthesis, cDNA library preparation using Nextera XT DNA Sample Preparation Kit (Illumina), sequencing on MiSeq platform using MiSeq Reagent Kit v2 (300 cycles) chemistry (Illumina) and paired-end sequencing.