Total DNA sequences of Azygia robusta
Libraries were prepared using an Ion Plus Fragment Library Kit and unique adapters from Ion Xpress Barcode Adaptors kit (ThermoFisher Scientific, Waltham, MA, USA) with pre-fragmentation on a Covaris M220 Focused-ultrasonicator. The emulsion PCR and template preparation were obtained on an Ion One Touch2 System (ThermoFisher Scientific) followed by sequence on an Ion S5 sequencing platform using Ion 540 chip at the Far Eastern Federal University (Vladivostok, Russia).