RNA seq of mus musculus: male ex vivo brain neocortex treated with ACSF replicate 1
Male and female 12 week old mouse brains were made into 450 um slices and incubated in artificial cerebrospinal fluid (ACSF). Extracellular field recordings were performed using either control ACSF or modified ACSF with 100um 4AP. After recordings, the neocortex was subdissected away from other tissue and flash frozen. The tissue was first homogenized in Trizol using an Omni bead ruptor homogenizer, and RNA was isolated using the Trizol/choloform-isopropanol method following the manufacturers instructions. RNA concentration and purity was determined using a a NanoDropOne. The libraries from each sample were then prepared using SmartSeq and Nextrera XT kits following the manufacturer's protocol.