WGS of Sample P2-C6A MPA01 cells treated with Ciprofloxacin
MPAO1 cells derived from sample P2-C6A were seeded from a frozen stock  in 5 ml of LB media containing 2 ug/ml ciprofloxacion and grown at 37C until saturation. Cells were collected and DNA was extracted using a Maxwell RSC Cultured Cells DNA Kit (Promega) and its quality determined in an Agilent 5200 Fragment Analyzer System using a Genomic DNA 50kb Kit (Agilent). Indexed pair-end libraries were prepared with TruSeq DNA Library Prep Kit (Illumina Inc, USA) and sequenced on a NextSeq 550 System Mid-Output Flowcell (Illumina Inc, USA) in a NextSeq 500 System Whole-Genome Sequencing Solution (Illumina Inc, USA). The resulting paired-end reads were 150 bp long.