RNA-seq of Citrus x changshan-huyou:ripe fruit
For RNA extraction, the tissue of the Huyou were processed using the Tengen DP411 kit, while the other samples (roots, stems, flowers, and fruits) were processed using the CLB+ Adderall RN40 kit according to the steps in the instruction manual. The mRNA library was constructed using the Dual-mode mRNA Library Prep Kit for Illumina (Hieff NGS Ultima) and subsequently sequenced on the Illumina NovaSeq6004 platform.