RNA-Seq of mus musculus:macrophages
There are two main ways to obtain mRNA: One is to use the structural characteristics of polyA tail in most mrnas of eukaryotes, and enrich the mRNA with polyA tail through Oligo(dT) magnetic beads. The second is to remove ribosomal RNA from the total RNA to obtain mRNA. Subsequently, the mRNA fragments were randomly interrupted with divalent cations in the NEB Fragmentation Buffer, and the mrna fragmentation was constructed according to the NEB common fragmentation or chain-specific fragmentation