Bulk RNA-Seq of Crotalus atrox Medial Spinal Cord - Sample CA3
Tissues were dissected from rostral (RSC), medial (MSC) (i.e. locomotor), and caudal (CSC, i.e. shaker) spinal cord from three individual Crotalus atrox. RNA was isolated from tissues using a Qiagen RNEasy Mini Kit following manufactuers instructions. Isolated total RNA was submitted to MSU RTSF Genomics Core, where Sequencing libraries were prepared using Illumina TruSeq Stranded mRNA Library Preparation Kit. Libraries were QCd and quantitated using a combination of Qubit dsDNA assay, Caliper LabChipGX and Kapa Illumina Library Quantification qPCR kits. The libraries within each project were pooled for multiplexed sequencing. Each pool was loaded on one lane of an Illumina HiSeq 2500 Rapid Run (v2) flow cell to target ~ 15million paired reads per sample. To reach this target, additional sequencing was performed on a MiSeq using the same read format, and results were pooled.