WGS of Acinetobacter phage LemonAid
30mL of phage filtrate was treated with a solution of DNase 1 (5mg/mL, Roche) and RNase A (10mg/mL Invitrogen) for 30 minutes at 37C to remove any remaining bacterial nucleic acids from the lysate. Polyethylene glycol 8000 (PEG) and NaCl were dissolved in the lysate to final concentrations of 10% w/v and 1.12M, respectively, and left overnight at 4C. Phages were concentrated by centrifugation at 10,000 x g for 10 minutes. The supernatant was removed and the phage pellet re-suspended in 1mL of SM buffer. DNA was purified using Promega Wizard kit following manufacturers instructions and quantified using the broad-range dsDNA Qubit assay and quality checked using Genomic DNA Screen Tape analysis on the Agilent 4200 TapeStation system, as per the manufacturers instructions. DNA sequencing libraries were prepared using NEBNext Ultra II FS Library Preparation and run on the Illumina Novaseq by the Exeter Sequencing Service, to generate 2x 150bp paired-end reads.