P. falciparum WGS: resistant W2
Ring-stage parasites were isolated with 0.1% saponin and were washed 3x with PBS. gDNA from resistant and parental strains were isolated using Circulomics Nanobind CBB high molecular weight DNA extraction kit. Pair-ended libraries were generated using the NEBNext Ultra II DNA PCR-Free Library prep kit. Libraries were sequenced on an Illumina NextSeq 2000 P3 using 2 x 150 bp paired-end sequencing (Chan Zuckerberg Biohub)