Targeted sequencing of Pinus sylvestris: needles
DNA extracted from needles (diploid tissue). DNA fragmented to 200 nt length with Bioruptor Ultrasonicator (Diagenode). Library preparation with NEBNext DNA Library Prep Master Mix Set for Illumina and multiplexing with NEBNext Multiplex Oligos for Illumina E7600S (New England BioLabs). Targeted capture with MycroArray MYbaits protocol v.2.3.1.