Whole-genome sequencing of the E. coli Q5 strain using MinION
E. coli Q5 and E. coli C41 genomic DNA was extracted from the overnight cultures grown at 37C using GeneJET Genomic DNA purification kit (ThermoFisher). gDNA was sequenced using Oxford Nanopore Technologies (ONT). Sequencing libraries were prepared from the non-sheared DNA using Native Barcoding kit (SQK-NBD114-24; ONT) with enrichment of long fragments using the LFB buffer according to the manufacturer's protocol. Sequencing was performed on MinION using the R10.4.1 flow cell (FLO-MIN114; ONT) with a translocation rate of 400 bps. Basecalling was performed using Guppy 6.0.1 (Wick et al., 2019) in the hac mode.