Pool-seq of larvae collected from Gishiri
Genomic DNA was extracted from between 8 and 12 adult individuals from each population. Individuals from each population were homogenized together in Eppendorf plastic tubes (1.5 mL) with polypropylene pestle. Thus, each DNA pool was a collection of samples from a specific midge population. Highly pure genomic DNA extraction was performed with NucleoSpin Tissue kit (Clontech Takara), according to the instructions of the manufacturer. Genomic DNA concentration was estimated using a Qubit 3.0 fluorometer with Quantifluor dsDNA system (Promega).