ITS amplicon seq of grapevine leaf: location 1_time point S5_treatment 2_rep2
Sequencing libraries were prepared by Microbiome Insight Inc. by using the high-fidelity Phusion polymerase for amplification of ITS2 marker gene. PCRs were done with dual-barcoded primers to enable multiplexing. PCR products were verified visually by running a representative subset of samples on a gel. The PCR reactions are cleaned up and normalized using the high-throughput SequalPrep 96-well Plate Kit. Samples are then pooled to make one library that is quantified with the KAPA qPCR Library Quant kit.