16S of B. distincta gut: Rest of midgut
A 450bp fragment of the V3/V4 section of the 16S ribosomal RNA region was amplified in all DNA samples using the Bakt_341F and Bakt_805R primer set (Klindworth et al., 2013). Amplicons were purified with Agencourt AMPure XP beads (Beckman Coulter, CA, USA). Further library preparation included addition of barcode sequences after which samples were combined for multiplex sequencing. Paired-end sequence data were generated using Illumina MiSeq technology.