fecal
The DNA samples were end-polished and A-tailed (NEBNext ultra II end repair kit. The end-prepared samples were ligated to PCR barcode adaptor (PCR) using Blunt TA ligase master mix (M0367L). The adaptor ligated samples were used in barcode PCR using 2X LongAmp master mix and barcode primer. The barcode details are listed in table 1. The equimolar concentration of barcoded sample was pooled and end prepared. Finally the sequencing adaptors (SQKLSK114) were ligated onto double-stranded DNA fragments using NEB Quick T4 DNA Ligase. After purification with AMPure XP beads, the prepared library was ready for library quality control.