Pseudomonas isolates from sediment treated with glyphosate
DNA was quantified using the Qubit 2.0 Fluorometer and integrity was verified on a 1% agarose gel. The NEBNext Ultra DNA Library Prep DNA Library Prep Kit for Illumina, clustering, and sequencing reagents were used following the manufacturers recommendations. DNA was fragmented by acoustic shearing and then end-repaired and adenylated. Adapters were ligated followed by enrichment using a limited cycle PCR.