Chloroplast-Seq of Castanopsis hystrix: growing fresh leaf
After the samples were qualified for genomic DNA detection, the DNA was interrupted by physical methods (ultrasonication), then the interrupted DNA was purified to construct sequencing libraries, the steps were as follows: DNA end repair, 3 'end with a, sequencing adaptor ligation, agarose gel electrophoresis to recover the target fragment, PCR amplification of the target fragment, and finally the construction of the sequencing library. The constructed library was quality checked before sequencing and after quality control the Illumina novaseq  platform was used for sequencing