Evaluation of genetic alterations in Heredeitary Cancer Susceptibility genes in the Ashkenazi Jewish Community of Mexico
DNA was extracted from peripheral blood DNA. The GeneRead Cancer Predisposition V2 Kit (Qiagen) was used to prepare the library, which targets 143 genes whose loss of function is a well-known mechanism associated with more than 80 inherited oncologic diseases based on data from the College of American Pathologists (CAP) guidelines, the National Comprehensive Cancer Network (NCCN) guidelines, and The Cancer Genome Atlas (TCGA). The amplification was carried out in four-pool PCR reactions and all libraries were barcoded and diluted equimolarly. Sequencing was performed in a HiSeq 4000 (Illumina) with pair-end (2x150) chemistry and to a theoretical coverage of 2000X.