LP05-1 WGS
1000 ng of gDNA was subjected to DNA sequencing library preparation using sparQ Frag1000 ng of gDNA was subjected to DNA sequencing library preparation using sparQ Frag& DNA Library Prep (QuantaBio, USA). Briefly, gDNA was fragmented using enzymatic reactionand cleaned with magnetic beads. An adaptor index was ligated to the fragmented DNA. Qualityand quantity of the indexed libraries were measured using Agilent 2100 Bioanalyzer and Denovixfluorometer and pooled in equimolar quantity. Cluster generation and paired end 2x250 nucleotideread sequencing were performed on Illumina MiSeq sequencer.