transcriptome in kidney of large yellow croaker
Total RNA was extracted from the kidney tissue of six large yellow croaker individuals which triplicate in 5 low salinity group (S-group) and 25 normal salinity group (C-group). The concentration and integrity of total RNA samples were measured using Qubit RNA Assay Kit in Qubit 2.0 Flurometer and 1.5% agarose gel electrophoresis, respectively. Whereafter, strand-specific RNA library was constructed using the NEB Next UltraTM RNA Library Prep Kit (NEB, MA, USA), and reads for library were purified using AMPure XP system (Beckman Coulter, CA, USA).