S10_greenstem_rep3
RNA extraction from Quillaja saponaria plants was performed using the MacKenzie-modified RNeasy Plant Mini Kit (Qiagen) protocol, with DNAase (Promega) treatment, performed on column. A Tecan plate reader was used to quantify the material using fluorimetry. The RQS was measured using a Perkin Elmer GX II. High-throughput NEXTflex directional RNA-seq library preparation (150bp, paired end) of each of the 24 samples were generated, which were multiplexed and sequenced over two lanes of a HiSeq 4000 PE150 instrument (Illumina).