Ribo_Nat10_DcKO3
We optimized a low-input Ribo-seq protocol adapted from a ligation and ultracentrifugation-free Ribo-seq approach, DNA fragments with15- to 35-bp insert bands were carefully excised and recovered using aDNA gel recovery kit, and the library sequencing was conducted on theNovaSeq 6000 platform with PE150 mode (Novagene).