PCR amplification and Oxford Nanopore Technologies sequencing of vmp expression site in Borrelia miyamotoi following in vitro antibody killing and reisolation.
The vmp expression site of B. miyamotoi LB2001 exposed to different antibody killing conditions and controls were PCR'ed with primers Pexp-F and Vlp25R and sequenced using ONT's Mk1B MinION with an R10.4 flow cell and the SQK-NCB112.24 library prep kit. The demultiplexing scheme is as follows: B01 Replicate 1 reisolate R2, B02 Replicate 1 no serum control (NSC), B03 Replicate 2 reisolate (R3), B04 round2 reisolate 1:5 high, B05 replicate 2 normal mouse serum control (NMSC), B06 Replicate 2 no serum control (NSC). A raw, concatenated FAST5 file is given here for the entire sequencing run.