DNA Virome of Puma concolor: feces
Viral DNA was extracted from the Puma concolor fecal sample using the High Pure Viral Nucleic Acid Kit (Roche Diagnostics, USA). The DNA extract was enriched for circular sequences with rolling circle amplification (RCA) using the Illustra TempliPhi Amplification Kit (GE Healthcare, USA), and sequencing libraries were prepared using the Nextera DNA Flex Library Prep Kit (Illumina, USA).