WGS of Helianthus tuberosus: young leaf
High-quality DNA was extracted from fresh leaf, quality checked by agarose gel electrophoresis and Nanodrop 2000 spectrophotometer, quantified by Qubit 2.0 with Qubit dsDNA HS Kit, sheared into 350 bp fragments by Covaris S220 with microTUBE, end-repaired, A-tailed, adapter ligated and purified by Illumina TruSeq DNA PCR-Free Library Prep Kit. The prepared sequencing library was quality checked by Agilent 2100 Bioanalyzer and quantified by Q-PCR before loading onto flowcell.