Extraction 1, miseq
Illumina MiSeq libraries were generated using Illumina Nextera XT library preparation kits (cat# FC-131-1096). All thirty libraries generated from both use cases were multiplexed together on a 2X300 paired end sequencing run using a 600 cycle MiSeq reagent kit (cat# MS-102-3003). All samples and use cases were sequenced as triplicates to achieve technical replication