UCE target enrichment of Eulaema_meriana _ML089.
Samples were extracted using column-based extraction kits. DNA was sheared to 400-600 bp using a QSoncia Q800R2 sonicator. Genomic libraries were prepared using Kapa Hyper Prep Kits (1/4 volume reactions) and iTru, 8bp dual-indexing adapters (Glenn et al. 2019). Samples were pooled into pools of ~10 samples and enriched for ultraconserved elements (UCEs) using an ant-customized RNA bait set (Arbor Biosciences; ant-specific hym-v2) that includes 9,898 baits (120 mer) targeting 2,524 UCE loci shared across Hymenoptera and a set of legacy markers (Branstetter et al. 2017). Enriched pools were pooled together (up to ~100 individual samples) and shipped to either the University of Utah genomics core facility or Novogene for sequencing on Illumina instruments.