TCR sequencing of CD8+ Human T-cells; activated with the Cytomegalovirus, Epstein-Barr Virus and Influenza Virus (CEF) control peptide pool
RNA-seq libraries were prepared according to the SCRB-seq/3'DGE protocol, using oligo-dT capture followed by template switching and barcoded cDNA generation. T-Cell Receptor transcripts were further enriched by targeted multiplexed PCR using published primer sets designed on the 5 end extremity of the variable region and the primer used for cDNA amplification. Final amplicons were prepared for Oxford Nanopore sequencing via ligation using Oxfords LSK-110 kit.