BCR-Seq of COVID-19 convalescent: P2, IgG & IgA
Peripheral blood mononuclear cells (PBMCs) were isolated from donor whole blood using Lymphocyte Separation Medium (Lonza). PBMCs were incubated with DNaseI, washed, lysed into 1mL Trizol and frozen. RNA was reverse transcribed with SuperScript IV (Invitrogen). IgG and IgA variable heavy chains were amplified for sequencing.