RNA-Seq of Micrcystis aeruginosa: P-limited
P-limited Microcysitis cells (grown in phosphorus-free medium) for transcriptomic analysis were collected on the 3rd day after inoculation. Total cellular RNA was extracted with RNAprep Pure Cell/Bacteria Kit (TIANGEN Biotech, Beijing, China) . The concentration and integrity of the extracted RNA were assessed by Qubit RNA Assay Kit in Qubit 2.0 Flurometer (Life Technologies, USA) and the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, USA). Library preparation and transcriptome sequencing were carried out by a commercial service company (Novogene Life Sciences Co., Ltd. Beijing, China).