HS27_p22um_AdvA_16S_2021
DNA extracted with Qiagen DNEasy Blood and Tissue kit. Amplified with NEXTFLEX 16S V4 Amplicon-Seq Kit 2.0 (custom to include Parada/Apprill modifications); full-length primers including sequencing primer are: FWD:GACGCTCTTCCGATCTTATGGTAATTGTGTGYCAGCMGCCGCGGTAA; REV:TGTGCTCTTCCGATCTAGTCAGTCAGCCGGACTACNVGGGTWTCTAAT.