RNA-Seq of Schizochytrium sp:mutant 24h
Trinity was used to de novo assemble the clean reads (removing PCR repeats to improve assembly efficiency), and then the assembled transcripts were clustered and de-redundant using Tgicl to obtain Unigene. Trinity contains three separate modules: Inchworm, Chrysalis and Butterfly, which process a large number of reads in turn. Trinity first constructs the reads into a large number of individual de Bruijn maps, and then extracts the full-length transcript isoforms for each map separately. transcript shear isoforms for each map.