WGS of P. aeruginosa
DNA extraction was carried out with the DNeasy UltraClean Microbial kit from Qiagen. Subsequently, libraries were prepared using the Nex-tera XT Kit from Illumina (San Diego, CA, USA) and subjected to sequencing on the Illumina MiSeq system, producing 2  301-base pair (bp) paired-end reads. The average depth of mapped reads was 98.49.9 (mean  the standard deviation) with a minimum of 81-fold, and the average breadth of coverage was 95.1%  1.0% using the PAO1 genome as reference.