Aurelia aurita microbiota from a host fitness experiment with bacteriophages
PCR amplicon libraries using uniquely barcoded primers flanking the V1-V2 hypervariable regions with fused MiSeq adapters and heterogeneity spacers were generated. Amplicons were size checked, purified, and quantified using the Qubit dsDNA BR Assay kit (Life Technologies, Darmstadt) and equal amounts of the purified PCR products were pooled for subsequent Illumina sequencing. Amplicon sequencing was performed on the Illumina MiSeq platform with v3 chemistry (2x300 cycle kit) according to the manufacturer's instructions.