RNA-seq of Malassezia furfur
Total RNA was isolated using Trizol Reagent (Invitrogen Life Technologies) and quantified using a NanoDrop spectrophotometer (Thermo Scientific). cDNA libraries were constructed from 3 g of RNA as input material, following standard protocols. mRNA was purified using poly-T oligo magnetic beads, fragmented, and reverse-transcribed us-ing random hexamers and SuperScript II. The second strand cDNA synthesis was per-formed using DNA Polymerase I and RNase H, followed by end repair, A-tailing, adapter ligation, and PCR amplification. The libraries were purified using AMPure XP beads and assessed using the Agilent 2100 bioanalyzer. Sequencing was performed on an Illumina platform.