RNA-seq of colorectal cancer patient-derived organoids
Total RNA (100ng) was prepared for Illumina RNA sequencing. Poly(A) RNA was purified using NEBNext Poly(A) Magnetic Module (New England Biolabs, #E7490) followed by library preparation using NEBNext Ultra II Directional RNA Library Prep Kit (#E7760S), according to the manufacturer's instructions. Libraries were quantitated by qPCR, pooled, and sequenced on an Illumina NextSeq 500 System running a 150 Cycle Mid Output Kit v2.