Shotgun sequence of museum specimens for mitogenome reconstruction
Extracted DNA performed in historic DNA laboratory specifically for museum specimens quantified via Qubit, Single Stranded SRSLY Pico plus library preparation from Claret Biosystems (iTru Style indices), qubit quantificaiton for pooling, shotgun sequencing, core demultiplexing, trimmomatic quality filtering and mapping reads to published mitogenome via Geneious Prime