Pool-seq of Acrocephalus arundinaceus: Early spring migrators
Genomic DNA was extracted and pooled in equimolar ratio for the ecotype. The pooled genomic DNA was randomly sheared into short fragments. Then DNA fragments were narrowly size selected with sample purification beads. The obtained fragments were end-repaired, A-tailed and further ligated with Illumina adapters. After these treatments, the fragments were filtered with beads again. The library was checked with Qubit and real-time PCR for quantification and Bioanalyzer for size distribution detection. Quantified libraries were pooled and sequenced on an Illumina Novoseq 6000, according to effective library concentration and data amount required.