Genome-seq of Acanthopagrusschlegelii: female parent
Quality-checked genomic DNA is enzymatically fragmented, repaired, A-tailed, and adapter-ligated. 300-400bp fragments are PCR enriched, purified with AMPure XP, and quantified via real-time PCR. Libraries are analyzed on Agilent 2100 Bioanalyzer before sequencing on NovaSeq 6000 with PE 150 strategy.