seq 16S rRNA genes of bacterial biofilm on microplastic in hypertrophic lake
Bacterial assemblages were analysed metagenomically based on the hypervariable V3-V4 region of the 16S rRNA gene. Specific primers 341F and 785R were used for amplification and further library preparation, while PCR was performed using Q5 Hot Start High-Fidelity 2X Master Mix.