DNA-Seq of K. marxianus 3444 strain
The extracted DNA is fragmented into smaller pieces, then the fragmented DNA ends are repaired to generate blunt ends suitable for adapter ligation. A single nucleotide adenine is added to the 3' ends of the DNA fragments. Sequencing adapters containing complementary overhangs are ligated to the DNA fragments. These adapters contain sequences necessary for subsequent steps such as PCR amplification and sequencing. The ligated DNA fragments are size-selected to ensure that only fragments of the desired length (e.g., 200-500 base pairs) are retained for sequencing. The size-selected DNA fragments are PCR amplified to enrich the library for sequences with adapters ligated on both ends. The validated DNA library is loaded onto a sequencing platform Illumina.