RNA-Seq of E. coli MG1655 in acid stress medium
Total RNA was extracted using the RNAprep Pure Bacteria Kit (TIANGEN) and rRNA was removed to isolate mRNA. The mRNA was randomly fragmented into small fragments (~200bp) and cDNA was then synthesized using the mRNA fragments as templates by the TruSeqTM Stranded Total RNA Library Prep Kit (Illumina). Flat ends of cDNA fragments were obtained by the End-Repair Mix (Qiagen). A single nucleotide adenine was added to the ends for linking with the adapter. The library was sequenced using Illumina HiSeqTM 2000 platform.