Oxford Nanopore Q20 400bps sequencing of adult D. melanogaster, duplex only
Nuclei were extracted from ~100 male adult flies and gDNA purified by phenol-chloroform extraction. Libraries were prepared with the Oxford Nanopore LSK114 ligation kit and sequenced on a MinION R10.4.1 flow cell at 400bps. Duplex basecalling was performed with Guppy 6.4.2 in super-accuracy mode using the default quality filter and duplex_tools for duplex read identification and filtering.