LMD Decapod hepanhamaparvovirus
Considering the extremely low quantity of DNA derived from few cells, the DNA was amplified using a whole genome amplification method and the SeqPlex DNA Amplification Kit (Sigma-Aldrich) following the manufacturers recommendations. The WGA DNA was sent to OmegaBioservices, Norcross, GA. Library for the DNA samples were generated at OmegaBioservices using the Library Kit, KAPA Hyper prep for WGS (Roche). The samples were sequenced using an Illumina HiSeq 2500 System