DNA-Seq of human subgingival microbiome: 42 days after uncovery
Biofilm samples were harvested from the peri-implant sulcus, as well as the gingival sulcus of adjoining teeth immediately after uncovery and at 1, 7, 21, 42, and 84 days following uncovery, by inserting a total of six sterile endodontic paper points into the peri-implant and the adjoining periodontal sulci for 30 seconds. DNA was isolated with a Qiagen DNA MiniAmp kit. Multiplex bacterial tag-encoded Whole Genome Shotgun Sequencing was performed using Illumina MiSeq paired-end platform