DNA Virome of Lynx rufus: feces
Viral DNA was extracted from the Lynx rufus fecal sample using the High Pure Viral Nucleic Acid Kit (Roche Diagnostics, USA). The DNA extract was enriched for circular sequences with rolling circle amplification (RCA) using the Illustra TempliPhi Amplification Kit (GE Healthcare, USA), and sequencing libraries were prepared using the Nextera DNA Flex Library Prep Kit (Illumina, USA).