Populus alba
The RNA-seq libraries were prepared by Genewiz (Azenta Life Sciences, Leipzig, Germany). Initially, mRNAs were enriched using Oligo(dT) beads and fragmented, followed by the synthesis of first-strand and second-strand cDNAs. Subsequently, cDNA fragments underwent end-repair, 3' end adenylation, and ligation of universal adapters. Index addition and library enrichment were carried out through limited-cycle PCR. RNA sequencing was conducted on an Illumina NovaSeq 6000 with a 2x150 pair-end configuration.