RIP-Seq of Flag-MEX3A in 293T cells
Antibody immunoprecipitation RNA was quality control with QubitTM(Thermo Fisher Scientifc,USA) and Agilent 2200 TapeStation (Agilent Technologies, USA). Then, 100ng RNA was used for library building following NEBNext Ultra RNA Library Prep Kit protocol for Illumina (NEB, USA). The final library product was assessed with Agilent 2200 TapeStation andQubit2.0(Life Technologies, USA) and then sequenced on Illuminaplatformat sing-end /pair end reads for 50bp/150bp.