Shotgun metagenomics of creek water
Metagenomic libraries of uncultured samples were constructed using the Nextera XT DNA Library Prep kit from Illumina. The quality of the libraries was determined with an Agilent 2100 BioAnalyzer and Agilent DNA High Sensitivity DNA kit and pooled. Sequences between 250 and 400 bp were selected via purification using a 2% agarose gel and the Qiagen QIAquick gel extraction kit.