Transcriptome sample of Carpoglyphus lactis
RNA samples were resuspended in 30ul water and 700ng RNA samples were used for rRNA removal by using Ribo-Zero Plus rRNA Depletion Kit. rRNA depleted samples were   used for library preparation using the KAPA mRNA HyperPrep Kits, the final concentration of all the libraries were measured  and the average library size was determined. The libraries were then pooled in equimolar ratios of 0.6nM, and sequenced paired end for 500 cycles.