Field Test of a scaled-up bioelectrochemical sequential reductive/oxidative process for Chlorinated Aliphatic Hydrocarbons (CAHs) contaminated groundwater removal
The extracted DNA was used for microbial characterization through 16S rRNA gene amplicon sequencing, conducted on the Rho groundwater and Inoculum I. Inoculum II was not included in this analysis as it was already characterized in a previous study (Matturro et al., 2018). The amplicon libraries were prepared and sequenced on a MiSeq instrument (Illumina, San Diego, CA, USA) using a MiSeq Reagent kit v3, 600 cycles (Illumina, USA), following standard guidelines as previously described [Matturro et al., 2021]. Bioinformatic analysis was performed after checking read quality with FastQC software (v 0.11.7). Sequences were processed, quality-filtered and analyzed using QIIME2 version 2018.2 [Boylen et al., 2018].  The DADA2 algorithm was used to denoise paired end sequences, dereplicate them and filter chimeras via the consensus method, and resolve amplicon sequence variants (ASVs) [Callahan et al., 2016]. The Silva 13299 database was used to assign the taxonomy (release December 2017, https://www.arb-silva.de/documentation/release-132/) [Quast et al., 2013].