P. pseudocerasus  Hi-C sequence
The construction of Hi-C libraries was carried out using leaf cells from Duiyingtao. The process started with cell fixation using formaldehyde, followed by cell lysis. We then subjected the cross-linked DNA to digestion with the DpnII enzyme. The resulting sticky ends were biotinylated and then proximity-ligated to form chimeric junctions. In the subsequent step, we enriched DNA fragments of size between 300-500 bp through a physical shearing process. These chimeric fragments, indicative of the original long-distance physical interactions within the cross-linked DNA, were then converted into paired-end sequencing libraries. The final stage involved sequencing the paired-end reads using the Illumina NovaSeq platform, resulting in a total of 145 Mb read pairs. To ensure data quality, we employed the fastp software1 to filter out low-quality reads from the raw sequencing data. After removing the duplicate reads, we obtained a total of 127 Mb read pairs to assemble chromosomal level genome