Principapillatus hitoyensis: salivary gland, PH_AB1; RNA-seq
Total RNA was extracted using TRIzol (Thermo Fischer, Steingrund, Germany) and the RNeasy MinElute Cleanup Kit (Qiagen, Hilden, Germany) according to the manufacturers protocols. Library preparation: purification of poly-A containing mRNA molecules from total RNA, mRNA fragmentation, random primed cDNA synthesis (strandspecific),adapter ligation and adapter specific PCR amplification; Sequencing: paired-end (2 x 150 bp)