single-cell RNA-seq of Merkel cell carcinoma
Single-cell TCR libraries were prepared following the 10X Genomics Single Cell V(D)J protocol.Specific PCR primers were designed, including R1_hTRDC (5'AGCTTGACAGCATTGTACTTCC), R1_hTRGC (5'TGTGTCGTTAGTCTTCATGGTGTTCC), R2_hTRDC (5'TCCTTCACCAGACAAGCGAC), R2_hTRGC. Cleanups and PCR conditions were identical to the 10x protocol, but y/g libraries required additional amplification cycles because of the rarity of y/g T cells compared to alpha Beta  T cells in most cell populations (5'GATCCCAGAATCGTGTTGCTC), and the forward primer (5'GATCTACACTCTTTCCCTACACGACGC.