Illumina NovaSeq 6000 Sequencing of Pan troglodytes isolate AG18354 Cell Line Genomic Paired-end Library BET0008_2
Illumina NovaSeq 6000 2x151 sequencing of Pan troglodytes isolate AG18354 Cell Line genomic paired-end dual index library. PCR-free libraries were generated from 1 microgram genomic DNA using a Covaris LE220-plus to shear the DNA and the TruSeq DNA PCR-Free HT Sample Preparation Kit (Illumina) for library generation. The median insert sizes were approximately 400 bp. Libraries were tagged with unique dual index DNA barcodes to allow pooling of libraries and minimize the impact of barcode hopping.