ddRADseq of Abies alba: leaf
Fresh leaf samples were collected from Abies alba individuals. 100 mg of each sample were lyophilized and used for total genomic DNA extraction with DNeasy Plant Mini Kit (Qiagen, Germany). PE libraries for ddRADseq were constructed using ApeKI and PstI restriction enzymes and sequenced by LGC Genomics (Germany), with a sequencing depth of 1M.