RNAseq of SCLC PDX: MGH1514-2A
Frozen tissue fragments were lysed and homogenized with the TissueLyser (Qiagen), and RNA extractions were performed with the Qiagen RNeasy Mini Kit. RNA quality was assessed with a 2100 Bioanalyzer DNA Chip 7500 (Agilent Technologies) and samples with an RNA integrity number of more than 8 were selected for library construction. cDNA libraries were prepared with the Illumina TruSeq Stranded mRNA Sample Kit and sequenced with a paired- end 2×75 bp protocol on an Illumina HiSeq 4000 instrument.