WGS_seq of Batrachium bungei: young fresh leaf
DNA was extracted from the leaf tissue using CTAB method. Then DNA quality and quantity were determined by the ND-2000 (NanoDrop Technologies). Only high-quality DNA sample (OD260/280=1.8~2.0, OD260/2302.0) was used to construct sequencing library. DNA-seq libraries were prepared following TruSeqTM Nano DNA Library Prep Kit from Illumina (San Diego, CA) using 1g of DNA. Shortly, and then fragmented by fragmentation buffer firstly. Secondly, fragmentated DNA was subjected to end-repairing, phosphorylation and A base addition according to Illuminas library construction protocol. After quantified by TBS380, paired-end DNA-seq sequencing library was sequenced with the Illumina NovaSeq 6000 sequencer (PE150).