gRNA screening for CYC1 promoter in Yeast, Group 3, Sequencing replicate 3
A pool of 19 gRNAs targeting CYC1 promoter was introduced into three yeast strains expressing different activation domains. Approximately 80 clones were randomly picked per activation domain. The clones were grown in 96-deep well culture. Their cell density and red fluorescence were quantified. Every strain had a red fluorescence protein cassette in its genome driven by a CYC1 promoter. Based on the fluorescence level, the cultures are splitted into five groups, with their plasmid are extracted for nanopore sequencing.