hESCs_VEGFA_1014bp_L_WT_2
The genomic sites were amplified and sequenced on the Illumina sequencing platform (Novaseq 6000), and two rounds of PCR reactions were used to obtain sequencing samples. In the first round of PCR (PCR1), the genomic target sites were amplified with a pair of specific primers containing Illumina forward and reverse connectors and 5-bp barcode using Phanta Max Super-Fidelity DNA Polymerase (Vazyme) and 25 PCR cycles. In the second round of PCR (PCR2), Illumina primer pairs containing 6 bp index were added to the reaction system by 12 PCR cycles. The PCR2 products were purified by HiPure PCR Pure Mini Kit (Magen).