RNA-Seq of Solanum lycopersicum pollen tube: Heinz, 28C, 60 minutes, replicate 2
RNA was extracted with the QIAGEN RNeasy Mini Plant Kit after cell lysis. For seedlings, plant material was ground up using a ceramic mortar and pestle that were frozen with liquid nitrogen. Three rounds of grinding as liquid nitrogen evaporated were performed - samples remained frozen throughout. Plant material was scraped from the mortar and pestle with a flash-frozen spatula into 1.5 ml Eppendorf tubes containing 450 l of buffer RLT with 1% -mercaptoethanol (-ME). Then the RNA extraction process was continued according to the RNeasy plant kit protocol.