HIFI CCS seq of Glyphodes pyloalis: Adult
To build the libraries, we used a commercial kit that prepares the DNA for long read sequencing. This involved fragmenting the genomic DNA into smaller pieces of around 20,000 base pairs. Adapters were added to the ends of each fragment containing Illumina sequencing primer sites. The DNA fragments with adapters were then purified and size selected to remove any undesirable small fragments. The prepared libraries were quantified using qPCR to measure concentration and make sure enough DNA was present for sequencing. Finally, the libraries were loaded onto proprietary HiFi sequencing flow cells and sequenced on the Revio platform.