PacBio HiFi sequencing data for Camponotus vicinus: male pupa individual
The HiFi SMRTbell library was constructed using the SMRTbell Express Template Prep Kit v2.0 (PacBio, Cat. #100-938-900) according to the manufacturer's instructions. HMW gDNA was sheared to a target DNA size distribution between 12 - 20 kb.  The sheared gDNA was concentrated using 1.8X of AMPure PB beads (Pacific Biosciences - PacBio, Menlo Park, CA; Cat. #100-265-900) for the removal of single-strand overhangs at 37C for 15 minutes, followed by further enzymatic steps of DNA damage repair at 37C for 30 minutes, end repair and A-tailing at 20C for 10 minutes and 65C for 30 minutes, and ligation of overhang adapter v3 at 20C for 60 minutes.  The SMRTbell library was purified and concentrated with 0.45X Ampure PB beads for size selection with 40% diluted AMPure PB beads (PacBio, Cat. #100-265-900) to remove short SMRTbell templates, <3 kb.  The 12-20 kb average HiFi SMRTbell library was sequenced at UC Davis DNA Technologies Core (Davis, CA) using two 8M SMRT cells, Sequel II sequencing chemistry 2.0, and 30-hour movies each on a PacBio Sequel II sequencer.