CUT&Tag Fresh U87-MG H3K27ac
Mixed U87-MG and DK-MG cells were subject to STAR-FACS to separate them based on TERT promoter mutations. The sorted fractions were used to perform CUT&Tag chromatin profiling with either H3K27ac antbody or H3K27me3 antibody. U87-MG and DK-MG fresh cells and nuclei isolated from fixed cells  were used as controls for CUT&Tag experiment.