WGS of Klebsiella pneumoniae C389: pure bacteria
Genomic DNA was extracted and fragmented into approximately 300bp fragments using the Covaris M220 system. These fragments were then ligated to a Y-shaped connector, while subsequently removing the self-ligated connectors using magnetic beads. To enrich the library template, PCR amplification was carried out. The DNA amplicons were then linearized into single strands and subjected to Bridge PCR. Finally, the modified DNA polymerase and dNTPs containing four fluorescent labels were introduced to the system for sequencing, which was performed using the Illumina HiSeq Nova6000 platform.