DMS-MaPseq of SARS-CoV-2; 1,799 nt segment; +Anti-LS2a ASO
RT with Induro (New England Biolabs); PCR with Q5 High-Fidelity 2X Master Mix (New England Biolabs); library generation with NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs)