RNA Seq of Streptomyces albidoflavus: Solid culture plate
Total RNA was extracted using the RNeasy Mini QIAcube kit (Qiagen; cat. No./ ID: 74116) and treated with DNase I according to the manufacturers instructions. RNA concentration and integrity were verified using Qubit 2.0 (Invitrogen) and Fragment analyzer (Agilent 530V). rRNA purification, library preparation and RNA-Seq were performed by Azenta Life Sciences. NEBNext rRNA Depletion kit (Bacteria) (New England Biolabs; cat. No. E7850X) was used for rRNA depletion. Barcoded libraries were prepared for each condition in duplicate with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina using default protocols. Libraries for all samples were sequenced using Illumina NovaSeq 6000.