CK_IP
ChIP assays were performed using an EpiQuikTM Plant ChIP kit (Epigentek) according to the user guide. The leaf tissues before and after drought treatment of 1 g were collected from 35S: BnaA9.NF-YA7-GFP transgenic seedlings grown in the pot for 4 weeks, respectively. An anti-GFP antibody (Abcam, ab290) was used to immunoprecipitate the sonicated DNA-protein complexes containing BnaA9.NF-YA7-GFP. The qualified chromatin fragments were sent to Novogene (www.novogene.com/) for ChIP-seq analysis. High-quality clean reads were mapped to the public B. napus genome Darmor-bzh v4.2 with default parameters using Burrows Wheeler Aligner (BWA)