RNA-Seq of HuH7 infected with EBOV for 24 h, Rep 2
HuH7 cells were  infected with nine different human pathogenic viruses. Samples were taken after 3 h, 6 h, 12 h and 24 h of infection. Control samples of uninfected cells were taken at the same time points. To determine the effects of virus replication on infectivity, cells were also exposed to BPL-inactivated virus for 24 h and finally uninfected cells were treated with BPL as an additional control. Total RNA was isolated using the Qiagen RNA Kit. The rRNA depletion and library preparation were completed using Illuminas TruSeq stranded total RNA kit.