RNA-Seq of MDSCs from MC38 tumor1
Total RNA was isolated from WT and Fgl2/ MDSCs in tumor-bearing mice on day 21 after tumor cell implantation. The RNA-seq library for these RNA samples was constructed according to the strand-specific RNA sequencing library preparation protocol. mRNA transcripts were enriched by two rounds of poly-(A+) selection with Dynabeads oligo-(dT) 25 (Invitrogen) before library construction. The prepared libraries were sequenced on an Illumina Novaseq 6000 platform.