RNA-seq of homo sapiens BEAS-2B, particulate matter exposure: Fine High 2
3 TagSeq was performed based on the protocols from Lohman BK et al. (2016) and Meyer E et al. (2011). Libraries were quantified using the Quant-it PicoGreen dsDNA assay (ThermoFisher) and pooled equally for subsequent size selection at 350-550bp on a 2% gel using the Blue Pippin (Sage Science). The final pools were checked for size and quality with the Bioanalyzer High Sensitivity DNA Kit (Agilent) and their concentrations were measured using the KAPA SYBR Fast qPCR kit (Roche). Samples were then sequenced on the NovaSeq 6000 (Illlumina) instrument with single-end, 100-bp reads.