scRNA-seq of mice retina in the OIR model2
The Single Cell 3' Protocol produced Illumina-ready sequencing libraries. A Single Cell 3' Library comprised standard Illumina paired-end constructs which begin and end with P5 and P7. The Single Cell 3' 16 bp 10x Barcode and 10 bp UMI were encoded in Read 1, while Read 2 was used to sequence the cDNA fragment. Sample index sequences were incorporated as the i7 index read. Read 1 and Read 2 were standard Illumina sequencing primer sites used in paired-end sequencing by  by Gene Denovo Biotechnology Co. (Guangzhou, China).