WGS of E. coli isolated from German soldiers stationed abroad
gDNA was isolated using Qiagen DNeasy Ultra Clean Microbial Kit. Library preparation was carried out using the NEBnext Ultra 2 FS DNA Library Prep Kit with the following steps: Fragmentation/End Prep, Adaptor Ligation, Size Selection of Adaptor-ligated DNA for DNA Input > 100 ng, PCR Enrichment of Adaptor-ligated DNA, Cleanup of PCR Reaction, Assessment Library Quality on a Qiagen QIAxcel and loading of the Illumina MiSeq using V3 chemistry and 2x250 bp paired-end sequecing.