WES of prostate cancer PDX
Sequencing libraries were prepared from 500 ng sheared DNA with the KAPA Hyper Prep Kit (Roche) according to the manufacturers standard protocol for end repair and A-tailing, adaptor ligation, high-fidelity PCR amplification, and cleanups. Exome enrichment of the DNA library (500 ng) was performed by hybrid capture with the IDT xGen Exome Research Panel (427,692 probes, 39 Mb target region, 19,396 genes; Integrated DNA Technologies).