Whole genome sequencing
overnight culture from single colony innoculated into 3ml rich media YPC18%.  Genomic DNA was extracted using P:C:I method . Purified DNA was quantified using a NanoDrop system (Thermo Scientific) and sonicated in a Diagenode sonicating water bath for 15 cycles. DNA quality was assessed by using a bioanalyzer using a high-sensitivity DNA chip (Agilent). Samples were submitted to Duke Center for Genomic and Computational Biology core sequencing facility for adapter ligation with TruSeq (Illumina) adapters and library amplification.