RNA-Seq of Drosophila melanogaster: adult CS CNS
The cleaved RNA fragments are copied into first strand cDNA using SuperScript II reverse transcriptase and random primers. This is followed by second strand cDNA synthesis using DNA Polymerase I and RNase H. The cDNA fragments then go through an end repair process, the addition of a single A base, and then ligation of the Illumina multiplexing adapters. The products are then purified and enriched with PCR to create the final cDNA sequencing library.