Metagenomics insights into the microbial resistome and virulome  composition of Kampalas wastewater
The metagenomes were prepared using the Illumina Nextera Flex kit designed for MiSeq+NextSeq, which demands a minute starting material (1.0 ng) due to its PCR-based library preparation method. Samples underwent tagmentation, involving enzymatic shearing and adaptor tagging, followed by PCR amplification with barcode incorporation. Subsequently, purification was performed using columns, normalization with Illumina beads, and pooling for loading onto the NextSeq platform.