Whole transcriptome sequencing of mus musculus: male thalamus targeting virus
Four weeks post injection, brains were dissected in 5 mM HEPES in HBSS and total RNA was isolated from individual brain regions using QIAzol reagent (Qiagen, #79306) and the Qiagen miRNeasy Kit reagent (Qiagen, #217004) according to the manufacturers instructions. Purified RNA was delivered to CeGaT for total RNAseq. The library was prepared with the KAPA RNA HyperPrep Library Prep Kit with RiboErase (HMR) and KAPA Globin Depletion Hybridization Oligos (Roche) and sequenced with a NovaSeq 6000 (50 M reads, 2  100bp pairedend; Illumina).