ExomeSeq of homo sapiens: Control2
The genomic DNA was randomly sheared into short fragments, end-repaired, A-tailed, and ligated with IDT xGen Stubby adapters, then barcode was added with UPI primers during pre-capture library PCR amplification.  Agilent all human exon probe V7 was used for capture step. The captured libraries were enriched by PCR amplification, and Qubit and bioanalyzer assay were used for final capture library quality control and quantification. The libraries were pooled and sequenced on Illumina Hiseq2500 using PE101 strategy.