RNA-Seq of alfalfa
Total RNA was extracted from alfalfa tissue samples of 'grassland No. 4'. The concentration and purity were detected by Nanodrop2000, RNA integrity was detected by agarose gel electrophoresis, and RIN value was determined by Agilent2100. After the samples were qualified, the library was constructed. The library was constructed and sequenced by Megi Biotechnology Co., LTD., which performed full-length transcriptome sequencing based on Single molecule real time sequencing (SMRT) technology and Sequel II(PacBio) high-throughput sequencing platform.