total RNAPII ChIP WT Col-0
Chromatin immunoprecipitation was performed using nuclei isolated from crosslinked (1% formaldehyde) 21-day old leaves. Chromatin was sonicated at 4C with a Diagenode Bioruptor Plus at high intensity for 30 min (30 s on/30 s off) to obtain 200-300 bp DNA fragment size. Antibodies against total RNA Pol II (Abcam ab817) were used with Dynabeads Protein G. DNA libraries were obtained using MicroPlex Library Preparation Kit (Diagenode).