RNA-Seq of Oryza sativa: panicles at the grain-filling stage
Fresh panicles werefrozen in liquid nitrogen immediately after harvest, transported by dry ice, and stored at 80 C . RNA was isolated from approximately 10 g of frozen panicles using TRIzol Reagent(Invitrogen) and treated with RNase-free DNase I (NEB, Hitchin, Hertfordshire, UK. toremove genomic DNA. Qualified RNA was used for library construction and sequencing.Library construction was completed using an mRNA-Seq Sample Prep Kit (Illumina, SanDiego, CA, USA) in accordance with the manufacturers instructions.