RNA-seq of Chicken: 4S3
Viral particles enrichment involved antibiotic treatment, homogenization, centrifugation, filtration and nuclease treatment procedures. Viral RNA extraction was extracted using QIAamp viral RNA mini kit (Qiagen, Hilden, Germany), DNA treatment employed, followed by a sample purification and concentration step before proceeding to library preparation. All constructed cDNA libraries from mixed viral RNA were sequenced on an MiSeq platform (Illumina, San Diego) using a V3 600 cycle kit.