PacBio Genome Sequencing of Xanthomonas oryzae pv. oryzae: Whole Genome
Use the G-tubes method to process the genomic DNA into 15-20k fragments, fill the ends, and connect the two ends to the circular single strand: the two ends of the single strand are connected to the positive and negative strands of the double strand respectively, and a dumbbell-like ("set") is obtained. SMRT Bell, the structure of the "horse ring"), anneals the single-stranded loop of the library and binds to the polymerase at the bottom of immobilized ZMW (zero-mode waveguides, zero-mode waveguide holes).