rRNA depleted metatranscriptome of 10L seawater sample
Total RNA was extracted using the Direct-zol RNA MiniPrep Plus kit (Zymo Research R2702), quantified using a Qubit fluorometer 1.0 (Thermofisher) and quality controlled using a Bioanalyzer 2100 (Agilent) prior to sequencing. Sequencing libraries were prepared by depleting rRNA using the ThermoFisher RiboMinus kit (Yeast+Bacteria;K15503, K15504) and shearing to ~225bp fragments. Libraries were sequenced (150x150 paired-end) on the Illumina NovaSeq 6000 platform using the S2 flow cell with 300 cycles at the Northwest Genomic Center (University of Washington, Seattle).