RNA-Seq of Glyphoglossus guttulatus eye
Total RNA was extracted from whole eyes using the Promega Total SV RNA Extraction kit (Promega). Tissue was homogenized in the prepared lysis buffer using the Qiagen Tissuelyser (10 minutes at 20 Hz). Messenger RNA library construction was performed using the Kapa HyperPrep mRNA Stranded with RiboErase kit (Roche). Each indexed sample was pooled in equimolar amounts and sequenced with paired end 150 bp reads on a NovaSeq6000 at the North Texas Genome Center (University of Texas at Arlington).