Hela WGS by pacBio
Up to 5 μg of DNA was used to prepare an SMRTbell library with a PacBio SMRTbell Express Template prep kit 2.0. Briefly, single-stranded DNA overhangs were removed, DNA damage was repaired by end-repair and A-tailing, PacBio adapters were ligated, desired size fragments were purified using AMPure PB beads, and resulting circular consensus sequence (CCS) HiFi libraries were sized selected in the 10- to 50-kb fragment range using a BluePippin system. LR-seq data were generated on one SMRT cell 8M with v2.0/v2.0 chemistry on a PacBio Sequel II instrument.