PCR of digested cattle manure and food waste with inoculum and iron oxide nano-additive
DNA was extracted in triplicate using ZymoBiomics MiniPrep extraction kits. The replicates were standardized to 2 ng/ul and pooled. The 16S rRNA V4-V5 region of the pooled, standardized extracts were amplified via PCR using the 515F-Y and 926R primers containing Illumina adapters. Amplicons were sequenced using the Illumina MiSeq platform via the paired-end method.