RNA-Seq of Aludens:Female control
RNA-Seq experiments were performed on a cohort of flies from the Control and Treated (exposed to desiccation for 24 hours) groups. For this, flies were collected and sexed four hours after they emerged and were maintained in separate cages. Two groups of five females and five males from both experimental groups were randomly formed and used for RNA extraction. Total RNA from each pool was extracted with TRI Reagent (Thermo Fisher Scientific), and RNA integrity was checked using a 1% agarose gel. Paired-end 2x100 libraries were made following standard procedures of the TruSeq library Sample Preparation kit and sequenced using an Illumina HiSeq 4000 platform at CINVESTAV (Irapuato, Gto. Mex.).