RNA-Seq of D283Med human medulloblastoma cells with knockout of ASNSD1-uORF using gRNA #1
This RNA library preparation method takes total purified RNA as input and enriches for polyadenylated (polyA+) transcripts using oligo-dT coated beads. PolyA+ RNA is subsequently fragmented using heat and Mg+ and reverse transcribed into cDNA using random priming. Illiumina adapters are ligated to dsDNA and PCR amplified.  Library preparation was done with the Roche KAPA mRNA Hyper Prep.