ITS- leaf biofilm
For the determination of the fungal rRNA sequences, the ITS region was amplified using the ITS1F (CTTGGTCATTTAGAGGAAGTAA) and ITS2R (GCTGCGTTCTTCATCGATGC) primers (22). PCR was conducted using the HotStarTaq Plus Master Mix Kit (QIAGEN) and carried out as follows: initial denaturation at 95C for 5 minutes, denaturation at 95C for 30 sec, annealing at 53C for 40 sec, and extension at 72C for 1 min for 30-35 cycles, followed by final elongation at 72C for 10 min. Paired-end sequencing (bTEFAP) was performed by Molecular research DNA (Shallowater, Texas, USA; http://www.mrdnalab.com) on an Illumina MiSeq sequencing platform (Illumina, San Diego, California, USA) following the manufacturer's instructions