Hi-C of gallus gallus DT40: SMC3-AID (no auxin)
DT-40-CDK1 knockout , transfected with Xenops laevis CDK1as and TIR1, has a CRISPR derived mAID-clover knockin at SMC3. These cells will synchronize at mitotic entrance after addition of 1NM-PP1. Addition of auxin will deplete SMC3-clover via TIR1 proteasome degradation.