BCRseq of mus musculus 869-20-210913
DNA was isolated using the DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany), including an RNAse digest. BCR sequencing libraries were prepared as described previously (50). Tapestation Bioanalyzer (Agilent, Santa Clara, CA, USA) was used to determine quality of sequencing libraries, which were sequenced on a MiSeq platform (Illumina, San Diego, CA, USA).