DNA of Vairimorpha bombi from Bombus terrestris abdomens (short reads - Novogene)
Whole abdomen of adult Bombus terrestris worker homogenised, filtered through repeat centrifugation. The resulting spore pellet was suspended in yeast cell lysis solution (Biosearch technologies) with 1.0 mm Zirconia/Silica beads (Thistle Scientific). This was then run in a TissueLyser II at 30 m/s for 45 seconds six times. We then centrifuged the sample at 14,000 rpm for two minutes, added 1ul of RNase A, incubated at 65oC for 15 minutes, and then incubated on ice for 15 minutes. The DNA was then extracted and purified following the DNeasy Blood and Tissue Kit (Qiagen). DNA was sent to Novogene for sequencing