16S rRNA gene V4 hypervariable region of HN16 replicate2
DNA extraction from the sediment samples (0.5 g each) was performed using the Magnetic Soil and Stool DNA Kit (TIANGEN, China) following the manufacturer's instructions. Amplification of bacterial 16S rRNA genes targeting the V4 hypervariable region using Earth Microbiome Project (EMP) standard primers 515F (5GTGCCAGCMGCCGCGGTAA3) and 806R (5GGACTACHVGGGTWTCTAAT3). High-throughput sequencing was performed using the Illumina NovaSeq 6000 sequencing platform (Novogene, China) with paired-end reads.