NGS short read DNA seq of Glyphodes pyloalis: Adult
Genomic DNA was extracted using the DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. DNA quantity and purity were assessed using a NanoDrop spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). Library construction was performed using the Illumina TruSeq DNA Sample Prep Kit (Illumina, San Diego, CA, USA), following the kit's protocol, which included initial DNA fragmentation by sonication, end-repairing, A-tailing, adapter ligation, and enrichment of the adapter-ligated DNA fragments by PCR. Upon library validation with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantification by qPCR, the libraries were sequenced on an Illumina HiSeq platform (Illumina, San Diego, CA, USA) to generate paired-end reads of 150 bp in length.