RNA-seq of Timema cristinae for methylatio comparison
Total RNA for each individual was extracted from the de-gutted half-body using the Quiacube animal tissue kit and protocol. Libraries were prepared for each individuals using the NEB mRNA stranded library preparation kit and protocol. A polyA selection was realized on each RNA extractions prior to library preparation to enrich the libraries in mRNA. Libraries were multiplexed and sequenced on one lane of HiSeq4000 to obtain 150 base pair paired-end reads. Libraries were tagged with unique i5 i7 index combination to allow assignment of read pairs to individuals.