RNA-seq of Panax japonicus
Four kinds of tissues (rhizomes, leaves, stems, and lateral roots) were acquired from one- to three-year-old Panax japonicus specimens. Following tissue dissection, samples were immediately snap frozen in liquid nitrogen. Total RNA was extracted using the Plant Total RNA Kit (MilliporeSigma). RNA-seq libraries for PacBio SMRT sequencing were prepared following the Iso-Seq Template Preparation for Sequel Systems protocol (PacBio). Sequencing of pooled, barcoded libraries was performed on a PacBio Sequel System instrument.