Single Cell RNA Sequencing Data from Organoids Derived From Human Mammary Epithelial Cells With EPP at 21 Days - Sample B
Mammary-derived organoids from non-identified, non-cancerous, human breast tissue were cultured within Matrigel domes, submerged in media containing 10% R-Spondin1 conditioned medium, 5 nmol/L Neuregulin 1 , 5 ng/mL FGF7, 20 ng/mL FGF10 , 5 ng/mL EGF , 100 ng/mL Noggin, 500 nmol/L A8301, 5 mol/L Y-27632, 500 nmol/L SB202190, 1 B27 supplement, 1.25 mmol/L N-acetylcysteine, 5 mmol/L nicotinamide, and 50 g/mL Primocin in ADF+++. Organoids were then treated with 66.6 ng/mL of 17--Estradiol, 200 ng/mL of progesterone  and 200 ng/mL of prolactin for 21 days