T-IL-2-1
1 g total RNA was used for following library preparation. The poly(A) mRNA isolation was performedusing Oligo(dT) beads. The mRNA fragmentation was performed using divalent cations and hightemperature. Priming was performed using Random Primers. First strand cDNA and the second-strandcDNA were synthesized. The purified double-stranded cDNA was then treated to repair both ends andadd a dA-tailing in one reaction, followed by a T-A ligation to add adaptors to both ends. Size selection ofAdaptor-ligated DNA was then performed using DNA Clean Beads. Each sample was then amplified byPCR using P5 and P7 primers and the PCR products were validated.