TurboID-CRaf Proximity Labeling
TurboID-CRaf was co-expressed in cells stably expressing the library of Flag-MCP-tagged BRaf mutants and MS2-circRNA barcodes. Prior to biotin labeling, the cells were split into multiple plates. This sample was treated with DMSO for 1 hr then treated with biotin for 5 min. A subsequent streptavidin enrichment was performed on the lysate and the co-enriched MS2-circRNA barcodes were extracted and sequenced. Corresponds to drug treatment and biotin labeling #2 (biological replicate #2).