RNA metavirome-seq of maize leaves
Metatranscriptome libraries were prepared following TruSeqTM RNA sample preparation Kit , using 5g of total RNA. Shortly, rRNA removal by Ribo-ZeroTM rRNA Removal Kits (Epicenter), fragmented using fragmentation buffer. cDNA synthesis, end repair, A-base addition and ligation of the NGS-indexed adaptors were performed according to NGSs protocol.