Single Cell RNAseq of Macaca Mulatta: adult bronchial alveolar lavages
Live macrophages and T cells were FACS sorted from BAL samples of SVV-infected rhesus macaques (n=3) at 0 and 7 DPC. At 7 DPC cDNA from each cell was subjected to qPCR for viral orfs ro determine if the cell is infected (orf positove for three replicates) or a bystander (orf negative for three replicates). DPC0, DPC 7 infected, and DPC 7 bystander cells were than subjected to the Smart-seq2 protocol for single cell library preperation.