16S rRNA gene sequencing of adult female mus musculus vagina
Adult female mice were administered 10 ug of recombinant toxic shock syndrome toxin (TSST1) or an attenuated version (S72A) to determine effects on innate vaginal microbiota; whole vaginal tract was dissected and DNA extracted with Qiagen power soil HTP kit, 16S hypervariable region V3-V4 PCR amplified and sequenced