Whole genome sequence of Bacillus subtilis MBL_J17
Purified DNA was fragmented, added to adapter sequences, and loaded onto a flow cell containing complementary sequences for adapters. Fragments of DNA were attached to anchors, followed by cluster generation and bridge amplification. Nucleotides with reversible fluorescent blockers were added by DNA polymerase one by one. A camera takes pictures of the surface after each step and figures out which building block was added by looking at the color of each spot on the surface. The Illumina sequencer generated raw images utilizing sequencing control software for system control and base calling through an integrated primary analysis software called RTA (Real Time Analysis). The BCL (base calls) binary was converted into FASTQ utilizing the Illumina package bcl2fastq.