WGS of Spilophora sp midgut junction
Stammera-Cassidinae symbiosis was characterized across 60 representative beetle species, from 13 tribes. Foregut-to-midgut junction, where symbiotic organs are commonly localized, was dissected from 1-3 individuals under molecular grade absolute ethanol. DNA was extracted using the QIAGEN DNeasy Blood & Tissue Kit with RNase treatment. Genomic DNA was fragmented to an average size of 300 bp using Covaris S2. Sheared DNA was purified by SPRI beads and used to construct DNAseq libraries using the NEBNext Ultra II DNA Library Prep Kit for Illumina.