transcriptomic sequencing of mouse: CTL1
Total RNA was extracted from TA (n = 8 per group) of mice using Trizol reagent (Invitrogen, CA, USA) according to the the manufacturers guidelines. Two rounds of purification were performed using Dynabeads Oligo (dT) (Thermo Scientific, CA, USA) for purifying mRNA from total RNA. Purified mRNA was fragmented under 94  for 57 min using the Magnesium RNA Fragmentation Module (e6150, NEB, USA). cDNA was reverse-transcribed from cleaved RNA fragments and used to establish the cDNA library. The transcriptome sequencing was performed with the Illumina Novaseq 6000 platform (Illumina, Inc., CA, USA).