Mutation Accumulation (MA) of Escherchia coli: WGS of mutL- ancestor
Genomic DNA was extracted from 1 mL of an overnight (~14 hours, shaking at 200 rpm, 37C) LB broth culture of the ancestral clone (ancestor of mutation accumulation lines), using the GenElute Bacterial Genomic DNA kit, Sigma-Aldrich, and  quantified using Qubit HS dsDNA assay, Invitrogen. A Paired-end library was prepared using the Illumina Nextera XT DNA library preparation kit per the manufacturers instructions, and sequenced on the Illumina MiSeq platform using the 2250bp paired-end V2 reaction chemistry.