genome of phage
(1) DNA fragmentation: The DNA fragment was produced by the M220 Focused-ultrasonicator (Covaris, USA) to a size of 300-500bp; (2) Library construction: Libraries for sequencing was constructed by using the TruSeq DNA Sample Prep Kit (Illumina, USA), and remove libraries outside the range of 400-600bp; (3) The library was sequenced using Illumina paired-end sequencing on the MiSeq platform as a part of a flowcell (2  250 cycles; Illumina), and the reads quality controlled and error corrected were as followed: Filter out the adapter sequence from reads, remove the non-A, G, C, and T bases at the 5 'end, filter out reads with N ratio greater than 10%, filter out reads with base (Q>=20) account for less than 40%, filter out the reads with size less than 75bp; (4) Genome assembly: the effective reads are assembled into contigs under the linux environment through overlap, and contigs are cyclically filled by SPAdes Genome Assembler version 3.1.0 until the complete scalfords are generated. The online BLASTp server was used to verify the predicted open reading frames (ORFs).