Illumina Novaseq of Elysia diomedea
250mg of tissue from each sample was used to extract the DNA, and for the sample (Elysia-diomedea-17-Mar-2022-San-Juan-de-La-Costta) that was stored in ethanol, first the ethanol from the tissue was evaporated at room temperature under the hood. DNA was extracted using DNeasy PowerSoil Kit (Qiagen) and the DNA was eluted in 100uL C6 solution (Qiagen). The initial concentration of DNA was evaluated using the Qubit dsDNA HS Assay Kit (Life Technologies) (Table 1). 50 ng DNA was used to prepare the libraries using Illumina DNA Prep, (M) Tagmentation library preparation kit (Illumina) following the manufacturer's user guide. The samples underwent the simultaneous fragmentation and addition of adapter sequences. These adapters are utilized during a limited-cycle PCR in which unique indices were added to the sample. Following the library preparation, the final concentration of the libraries (Table 1) was measured using the Qubit dsDNA HS Assay Kit (Life Technologies), and the average library size (Table 1) was determined using the Agilent 2100 Bioanalyzer (Agilent Technologies). The libraries were then pooled in equimolar ratios of 0.6nM, and sequenced paired end for 500 cycles using the NovaSeq 6000 system (Illumina).