V4 16S rRNA gene seqeuncing of southern hairy-nosed wombat faeces
Microbial DNA was extracted from the samples using the DNeasy PowerSoil Pro DNA isolation kit (QIAGEN) as per the manufacturers instructions. Samples preserved in ethanol were centrifuged at 10,000 g for 5 minutes before pouring the ethanol off. Following this, 0.25 grams (g) of faeces were added to the 750 microlitres (l) bead bed. Single PCR reactions were performed for all extracted wombat faecal DNA using the reverse-barcoded V4 region 515F-806R primer pair (515F 5-GTGCCAGCMGCCGCGGTAA-3 and 806R 5-GGACTACHVHH HTWTCTAAT-3)