Low-coverage whole genome sequencing of diverse Dioscorea bulbifera accessions
Approximately 1 g genomic DNA was broken into small fragments using the Covaris E210 sonicator (Covaris Inc., MA, USA). Fragments were then size-selected selected by Agencourt AMPure XP-Medium kit (Thermo Fisher Scientific, USA) to attain sizes ranging from 200 to 400 bp. Following end repair, 3adenylation, adaptor ligation, PCR amplification, and purification, the resulting double-stranded PCR products were transformed into single-stranded circular DNA (ssCir DNA), through heat denaturation and circularization using a splint oligo sequence. The ssCir DNA was formatted as the final library, and sequenced on DNBSEQ-T7 sequencing platform to generate 150-bp paired-end reads.