Prokaryotic community composition of Lake Balaton open water sample, Siofok Basin collected on 06 August 2018
A part of the 16S rRNA gene was amplified with fusion primers containing Bacteria-specific sequences Bakt_341F (5'-CCT ACG GGN GGC WGC AG-3', Herlemann et al. 2011 ISME J) and modified Bakt_805R (5'-GAC TAC NVG GGT ATC TAA TCC-3', Herlemann et al. 2011 ISME J; Takahashi et al. 2014 Plos One), and Archaea-specific A519F (5'-CAG CMG CCG CGG TAA-3' and Arch855R (5'-TCC CCC GCC AAT TCC TTT AA-3') (Klindworth et al. 2013 Nucleic Acids Res) with Fluidigm CS1 and CS2 universal sequences at their 5' ends. The resulting PCR products were mixed before sequencing in 1:1 ratio.