16s V3 V4 amplicon sequence; T7 HKJ treated soil day 1
DNA was extracted from soil samples using the Hi PurA Soil DNA isolation kit. The V3-V4 regions of the 16S rRNA gene were amplified using primers'16S F  GCCTACGGGNGGCWGCAG and  16 S R - ACTACHVGGGTATCTAATCC'. Libraries were prepared using the Illumina Nextera XT kit. Sequencing was performed on an Illumina MiSeq platform with 2  300 bp paired-end reads.