Metagenomic seq of wetland Mukhrino bog near Khanty-Mansiysk
The purified DNA isolates were amplified with universal multiplex primers 341F (5-CCTACGGGNGGCWGCAG-3) and 805R (5-GACTACHVGGGTATCTAATCC-3) targeting variable V3-V4 region of 16S SSU rRNA (Klindworth et al., 2013). Further preparation of the 16S rRNA gene libraries was carried out as described in the MiSeq Reagent Kit Preparation Guide (Illumina, USA). Sequencing of 16S rRNA gene amplicons was carried out by Evrogen (Moscow, Russia) on an Illumina MiSeq platform using MiSeq Reagent Kit v2 (500 cycles) with paired-end 2  251 cycle sequencing mode.