DNA-seq of WT
One technical replicate was randomly chosen for each biological replicate. In total 9 samples were subject to whole-genome sequencing to identify mutations. For each sample, genomic DNA was extracted from ~108 yeast cells by Omega Yeast DNA kit (D3370-01). The whole genome sequencing was performed using the paired-end strategy on Illumina HiSeq at Genwiz following the standard procedure.