single cell isolate PN5N
Amplification was perfomed using mix of NEB Phi 29 enzyme and reaction buffer with an 8 hours MDA. Enzymatic fragmention was done using the NEBNext Ultra II FS DNA Library Prep Kit. The adapter ligation was done with dual combinatorial barcoding (20 adapters of 8 base pairs; 12 in columns, 8 in rows). Final PCR was performed with NEBNext ultra II Q5 master kit and standard Illumina index primers with double indexing (IDT-DNA). The libraries were sequenced on a Nextseq 2000 sequencer with a P2 600 cycles flow cell (Illumina)