Mitochondrial genome sequence analysis of sea cucumber Apostichopus japonicus in Pingtan County Waters, Fujian province
After the sample genome DNA is tested to be qualified, break the DNA with enzyme, and then purify the broken DNA to build a sequencing library. The steps are as follows: repair the DNA end, add A to the 3 'end, connect the sequencing connector, recover the target segment by agarose gel electrophoresis, and conduct PCR amplification on the target segment to finally build a sequencing library. Before sequencing, perform quality inspection on the constructed library. After passing the inspection, use Illumina Novaseq TM platform for sequencing