RNA-seq of human immortalized keratinocytes: Anti-photoaging effect of ferulic acid in conjunction with retinol on UVB-damaged Hacat cells
The polyA tail mRNA was enriched by Oligo(dT) beads, and the mRNA was randomly interrupted in Fragmentation Buffer. the M-MuLV reverse transcriptase system were used to synthesize double-stranded cDNA. The cDNA of about 370 bp to 420 bp was screened by AMPure XP beads, PCR amplification was performed, and PCR products were purified by AMPure XP beads.