18S metabarcoding of protistan community StB4
V4 region of the 18S SSU rRNA gene was targeted using the primers TAReuk454FWD1 and TAReukREV3. The libraries were pooled by equal molarity. Denaturation and sample loading were performed according to the Illumina Sample Preparation Guide using a MiSeqReagent Kit v3, 600 cycles (Illumina, USA).