amplicon sequencing 18s
purified PCR product is used to prepare an Illumina DNA library. Sequencing was performed at MR DNA (www.mrdnalab.com, Shallowater, TX, USA) on a MiSeq following the manufacturers guidelines. Sequence data were processed using MR DNA analysis pipeline (MR DNA, Shallowater, TX, USA).  In summary, sequences are joined, sequences <150bp removed, and sequences with ambiguous base calls removed. Sequences were quality filtered using a maximum expected error threshold of 1.0 and dereplicated. The dereplicated or unique sequences are denoised; unique sequences identified with sequencing and/or PCR point errors and removed, followed by chimera removal, thereby providing a denoised sequence or zOTU