Genome assembly of Neopusillimonas maritima 17-4A
Genomic DNA was extracted from fresh leaf tissue using the CTAB Method. DNA quality and concentration were assessed using a NanoDrop spectrophotometer and Qubit fluorometer (Thermo Fisher Scientific). High molecular weight DNA (approximately 1 g) was used without fragmentation to maintain long read lengths. DNA was end-repaired and dA-tailed using the NEBNext FFPE DNA Repair Mix and NEBNext Ultra II End Repair/dA-Tailing Module (New England Biolabs). Adapter ligation was performed using the Oxford Nanopore Ligation Sequencing Kit (SQK-LSK109) following the manufacturer's protocol. The ligated products were purified using AMPure XP beads (Beckman Coulter) to remove any unligated adapters and small fragments. Library quality was assessed using a NanoDrop spectrophotometer. Sequencing was conducted on an Oxford Nanopore MinION device using an R9.4.1 flow cell, following standard operating procedures provided by Oxford Nanopore Technologies.