Gut_microbiome_of_Chinook_salmon_sample_B266
The V5-V6 hypervariable regions of the 16S rRNA gene was amplified using UniA-V5F (ACCTGCCTGCCGATTAGATACCCNGGTAG) and UniB-V6R (ACGCCACCGAGCCGACAGCCATGCANCACCT) primers. The target sequence was prepared for high throughput sequencing (HTS) using a two-step polymerase chain reaction (PCR).