ICPC Lung Cancer East Asia, Tumor Whole Exome Sequencing: Sample P111-T-WES
Genomic DNA was isolated from frozen tumor and adjacent normal tissues using AllPrep DNA/RNA/miRNA Universal Kit (Qiagen). DNA quality was confirmed using Nanodrop 2000 spectrometer (Thermo Fisher). Whole exome sequencing (WES) were performed using 400 ng of purified genomic DNA by DNeasy PowerClean Cleanup Kit (Qiagen) from tumor and adjacent normal tissues for library preparation. SureSelectXT2 target Enrichment System (Agilent Technologies) was used for exome hybrid capture of WES followed by paired-end multiplexed sequencing. The concentrations of libraries were quantified by Qubit assays (Thermo Fisher) and the sizes of libraries were measured by Agilent TapeStation (Agilent). The paired-end sequencing was performed using HiSeq 4000 (Illumina).