arable_medium_salinity 16S
Metataxonomic analysis of soil bacterial biota was performed by analysing hypervariable regions V3-V5 of the 16S rRNA gene with the use of the primers S-D-Bact-0341-b-S-17 (5'-CCTACGGGNGGCWG-CAG-3', forward) and S-D-Bact-0785-a-A-21 (5'-GACTACHVGGG-TATCTAATCC-3', reverse) (Klindworth et al., 2013, Nucleic Acids Res. 41 (1), e1). The libraries were prepared following the Illumina Support Centre (ISC) protocol with a minor modification (2  Phanta Max Master Mix, Vazyme Biotech, Nanjing City, China was applied instead of Kapa Hifi Hot Start Ready mix). Sequencing was performed by Biobank (University of Lodz, Poland) on a MiSeq (Illumina, San Diego, CA, USA) using MiSeq Reagent Kit v2 (500 cycles) in pair read mode (2  250 bp).