RNAseq of Danio rerio adad1 missense wildtype sibling: male testes
Total RNA was isolated from 45 dpf adad1Y67X Tg [ziwi:eGFP]uc1 and adad1M392K Tg [ddx4:eGFP] testes using the RNeasy micro kit (Qiagen). Since the testes were very small at this age, 5 testes were pooled together for each biological replicate (4 biological replicates for each genotype). Isolated RNA was sent to Genewiz at Azenta Life Sciences, NJ for library preparation with poly-A selection method and 150-bp paired end sequencing on an Illumina HiSeq platform.