RNA-Seq of mus musculus: male
mRNA was isolated by oligo(dT) beads and then fragmented by fragmentation buffer. Double-stranded cDNA was synthesized using a SuperScript double-stranded cDNA synthesis kit with random hexamer primers. Then libraries were size selected for cDNA target fragments of 300 bp followed by PCR amplification using Phusion DNA polymerase for 15 PCR cycles. After quantified by TBS380, paired-end RNA-seq sequencing library was sequenced with the Illumina HiSeq 4011 sequencer.