WGS of one clone from evolved population 6 in E122
For each strain, after overnight growth in the SC medium, genomic DNA was extracted from around 107 yeast cells using a MasterPure Yeast DNA Purification Kit (Lucigen; MPY80200).  Sequencing libraries were constructed using Nextera DNA Flex Library Prep (Illumina; 20018705).  Samples were sequenced using an Illumina HiSeq X with a paired-end 150-nucleotide strategy.