PacBio IsoSeq of Hamiltosporidium tvaerminnensis: FI-OER-3-3
cDNA from the extraction was prepared using the NEBNext Single Cell/Low Input cDNA Synthesis and Amplification Module kit (New England Biolabs). The Iso-Seq cDNA library was generated with the Iso-Seq Express Oligo Kit and the SMRTbell Express Template Prep Kit 2.0 (PacBio) and sequenced by the Quantitative Genomics Facility service platform at the Department of Biosystem Science and Engineering (D-BSSE, ETH), in Basel, Switzerland, on an Sequel I.