ITS2 fragment amplicon seq, root-affected soil of Malus genotype MAL0130, replant diseased soil from site RU
PCR amplification of the fungal ITS2 region using primers gITS7/ITS4 (White et al. 1990; Ihrmark et al. 2012), library preparation from purified PCR products and sequencing according to Novogene Co (Cambridge, UK) standard procedures