WGS of agricultural soils: ControlT7
For days 0, 3, 5, 7, 10, and 21, we obtained a metagenome for each treatment from a single sample (n = 26 metagenomes). For this, genomic DNA was prepared for metagenomic sequencing using the ZymoBiomics DNA/RNA Miniprep kit and the Tecan Ovation Ultralow System V2 library prep kit and was sequenced at the University of Colorado Anschutz Sequencing Core on the Illumina NovaSeq 6000 with 2x150bp chemistry at 20 million read pairs per sample.