RNA-Seq of Bactericera cockerelli: Ca. Liberibacter-uninfected bacteriome 1
Total RNA was extracted from the pooled bacteriomes and corresponding body tissue samples. Strand-specific and barcode indexed RNA-seq libraries were generated from 300 ng of total RNA for each sample using Kapa mRNA Stranded library preparation kit (KK8421, Kapa Biosystems, Cape Town, South Africa), followed by 12 cycles of PCR amplification. The pool was quantified by qPCR with a Kapa Library Quant kit (Kapa Biosystems) and sequenced on 1 lane of the Illumina NovaSeq S4 platform (Illumina, San Diego, CA) with paired-end 150bp reads.