16S metabarcoding of bacterial community StB2
V3-V4 regions of 16S rRNA genes were amplified by PCR using primers for bacteria S-D-Bact-0341-b-S-17 and S-D-Bact-0785-a-A-21. The libraries were pooled by equal molarity. Denaturation and sample loading were performed according to the Illumina Sample Preparation Guide using a MiSeqReagent Kit v3, 600 cycles (Illumina, USA).