single cell isolate SQQ4
Amplification was perfomed using RTEPLI_g kit from QIAGEN with a 4 hour MDA. Enzymatic fragmention was done using the QIAseq FX DNA library kit . The adapter ligation was done with dual combinatorial barcoding (20 adapters of 8 base pairs; 12 in columns, 8 in rows). Final PCR was performed with NEBNext ultra II Q5 master kit and standard Illumina index primers with double indexing (IDT-DNA). The libraries were sequenced on a Nextseq 2000 sequencer with a P2 600 cycles flow cell (Illumina)