pErk Reporter Assay
Flag-tdMCP-Erk2 was co-expressed in cells stably expressing the library of untagged BRaf mutants and MS2-circRNA barcodes. Cells were lysed and the lysate split. A subsequent flag-IP was performed on this sample and the co-enriched MS2-circRNA barcodes were extracted and sequenced. Corresponds to cell transfection #2 (biological replicate #2).