FKCPDNA
Next generation sequencing library preparations were constructed following the manufacturers protocol. 200g genomic DNA was randomly fragmented by Covaris to an average size of 300-350bp. The fragments were treated with End Prep Enzyme Mix for end repairing, 5 Phosphorylation and 3 adenylated, to add adaptors to both ends. Size selection of Adaptor-ligated DNA was then performed by DNA Cleanup beads. Each sample was then amplified by PCR for 8 cycles using P5 and P7 primers, with both primers carrying sequences which can anneal with flowcell to perform bridge PCR and P7 primer carrying a six-base index allowing for multiplexing. The PCR products were cleaned up and validated using an Agilent 2100 Bioanalyzer. The qualified libraries were sequenced pair end PE150 on the illumina HiseqXten/Novaseq / MGI2000 System.