Mitochondrial DNA sequencing of homo sapiens: Cisplatin-resistant cells derived from OSCC SAS cell line (Native)
Both nuclear and mitochondrial DNA (mtDNA) were extracted using a QIAprep Miniprep Kit (QIAGEN, Germany). The mtDNA fraction was enriched and size-selected using solid-phase reversible immobilization (SPRI) paramagnetic beads (Agencourt AMPure XP; Beckman Coulter Inc., USA). The DNA samples were treated with BamHI (Vivantis Technologies, Malaysia) to linearise mtDNA. The DNA libraries were prepared using the 1D2 Ligation Sequencing Kit (SQK-LSK308; Oxford Nanopore Technologies, UK).