RNAseq of Cotesia congregata : ovaries stage 3 sample 2
Total mRNAs were extracted using the RNA isolation protocol from NucleoSpin RNA XS kit (Macherey-Nagel) and quantified on a Qubit 2.0 with a RNA HS Assay kit (Invitrogen) and on a Bioanalyzer 2100 with a RNA 6000 Pico Assay kit (Agilent). cDNA were constructed using the SMARTer-Seq v4 Ultra low Input RNA kit (ClonTech). The libraries were constructed using the NEBNext Ultra II kit, and were sequenced with an Illumina HiSeq2500