wasteland_medium_salinity ITS2
The fungal biota was evaluated by analysing the internal transcribed spacer (ITS2) with the use of the following primers: 5-GCATCGATGAAGAACGCAGC-3' (ITS3 forward, without overhangs) and 5- TCCTCCGCTTATTGATATGC-3' (ITS4 reverse, without overhangs) according to White et al. (PCR Protocols: A Guide to Methods and Applications, 1990. Academic Press Inc., New York, pp. 315322). The libraries were prepared following the Illumina Support Centre (ISC) protocol with a minor modification (2  Phanta Max Master Mix, Vazyme Biotech, Nanjing City, China was applied instead of Kapa Hifi Hot Start Ready mix). Sequencing was performed by Biobank (University of Lodz, Poland) on a MiSeq (Illumina, San Diego, CA, USA) using MiSeq Reagent Kit v2 (500 cycles) in pair read mode (2  250 bp)