16S rRNA GENE AMPLICON SEQUENCING OF PRISTINE CONTROL SITE OF MINI RIVER
Quality and quantity of metagenomic DNA samples were checked using NanoDrop and agarose gel electrophoresis. The QC passed samples were processed for first amplicon generation followed by NGS library preparation using Nextera XT Index Kit (Illumina Inc.). The QC passed libraries were sequenced on illumina MiSeq platform using 2 x 300 bp chemistry.