Sequencing of phage lysate from donor E.coli
Library preparation, including DNA tagmentation, PCR-mediated adaptor addition and amplification of the adapted libraries, was performed following the Nextera XT library preparation protocol (Illumina), as previously described49. Libraries were confirmed using Fragment Analyzer (Agilent Technologies) and sequencing performed using the Illumina NextSeq 2000, obtaining 2  65 bp pair-end reads. Library preparation and sequencing were performed by the Genomics Unit at the Instituto Gulbenkian Cincia