Targeted-capture sequencing of human genomic DNA: blood
NA was extracted from PBMCs that had a tumor purity >80% or otherwise from PBMCs enriched for CD5+/CD19+ clonal B-cells. After sample quality control, the entire coding regions of 59 somatically recurring mutated CLL genes were sequenced using a customized SureSelect panel. Samples were paired-end sequenced (150 bp reads), using Illumina HiSeq 4000 sequencer.