Clarification of cryptic species of Plethodon in Tuscaloosa County, Alabama, USA
DNA extracts containing between 1-8 ug (average: 6.8 ug) of DNA were sent to RAPiD Genomics (Gainesville, Florida) for library preparation, sequence capture, and sequencing. Samples were barcoded using standard Illumina TruSeq adapters with a unique 8 bp index for each individual. We used the tetrapods UCE probe set of 5,472 probes (see Faircloth et al. 2012 and http://ultraconserved.org for probe development).