RNA-Seq of poplar: stems of control plants
The mRNA was enriched with magnetic beads containing Oligo (dT), and randomly interrupt mRNA by adding Fragmentation Buffer. The  first cDNA and second strand were synthesized Using mRNA as a template, and  the purified double stranded cDNA is subjected to end repair, A-tail addition and sequencing adapters connection, followed by fragment size selection using AMPure XP beads. Finally, a cDNA library was obtained through PCR enrichment.