Metagenome sequencing of pheasant gut
After sequence-independent amplification by random primed RT-PCR the amplicons were cleaned up with Gel/PCR DNA Fragments Extraction kit (Geneaid Biotech Ltd., Taipei, Taiwan). Libraries for next-generation sequencing were prepared with Nextera XT DNA Sample preparation kit (Illumina, San Diego, CA, USA) and subjected to se-quencing on Illumina NextSeq 500 platform according to the previously described protocol (Bali et al., 2021).