PDAC_PDO_Gem_R_RNAseq_rep1
Total RNA was extracted with TRIzol Reagent (Life Technologies, CA, USA) according to the manufacturer's protocol, and RNA integrity was evaluated using Agilent Bioanalyzer 2100 (Agilent Technologies, CA, USA) to obtain RIN values. RNA with RIN values > 7 was purified using an RNAClean XP Kit (Beckman Coulter, Inc. CA, USA) and RNase-Free DNase Set (QIAGEN, GmBH, Germany). RNA libraries were prepared for sequencing with VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina (Vazyme, Nanjing, China), and sequencing was conducted on an Illumina HiSeq 2500 system.