scRNA-seq of mus musculus: adult siLP immune cells
Chromium Single Cell 3 Gene Expression (GEX) libraries with paired Cell Multiplexing Oligo (CMO) libraries were provided by the Investigator. The libraries were assessed for size distribution on the Agilent Bioanalyser (Agilent Technologies, #G2939AA) with the DNA HS Kit (#5067-4626), and quantified using the Qubit 2.0 Fluorometer (Thermo Fisher Scientific Inc, #Q32866) and the Qubit dsDNA HS assay Kit (#Q32851). Library molarity for sequencing was calculated using the Qubit dsDNA quantification results and the fragment size information from the Bioanalyser results.