Subject 16 Tumor RNA sample at timepoint = POST2, sequenced in batch 2
RNA was extracted from frozen tumor sections using the Qiagen AllPrep DNA-RNA Mini Kit (80204). Library preparation was performed by the Translational Immunogenomics Lab (TIGL) at the Broad Institute and Dana-Farber Cancer Institute using the New England BioLabs NEBNext Single Cell-Low Input RNA Library Prep Kit for Illumina (E6420L). TIGL then performed paired 2x150bp sequencing using NovaSeq6000 S4 (Illumina).