WGS of Mus musculus: female medulloblastoma
Whole genome sequencing (WGS) libraries were prepared using the Truseq DNA PCR-free Library Preparation Kit (Illumina) in accordance with the manufacturers instructions. Briefly, 1ug of DNA was sheared using a Covaris LE220 sonicator (adaptive focused acoustics). DNA fragments underwent bead-based size selection and were subsequently end-repaired, adenylated, and ligated to Illumina sequencing adapters.  Final libraries were quantified using the Qubit Fluorometer (Life Technologies) or Spectromax M2 (Molecular Devices) and Fragment Analyzer (Advanced Analytical) or Agilent 2100 BioAnalyzer. Libraries were sequenced on an Illumina Novaseq6000 sequencer using 2x150bp cycles.