Target enrichment of Psychotria guianensis
We extracted total genomic DNA from leaf tissue using a sorbitol extraction protocol adapted from Storchova et al. (2000). DNA concentration was assessed with a Qubit fluorometer, and fragment size distribution was visualized with gel electrophoresis (1% agarose). Sample extractions that yielded low DNA concentrations were repeated and then pooled together in order to isolate enough DNA for sequencing (i.e., 280 ng). Probe design, library preparation, and sequencing were conducted by RapidGenomics (Gainesville, FL, USA). Using 2270 identified exonic regions as templates and a proprietary workflow, RapidGenomics synthesized 14,429 biotinylated 120-mer RNA probes in an overlapping strategy to reach full coverage of each locus. Following library preparation and target enrichment, 150-bp paired-end read sequencing was conducted in one lane of an Illumina NovaSeq S4.