RNA-Seq of Mytilus edulis whole tissues
Whole mussels were homogenized into RNAlater solution using a tissue homogenizer. RNA was isolated using Qiagen RNeasy mini kit using the manufacturers protocol and with an additional on column DNase I treatment. Concentration of RNA was assessed using nanodrop spectrophotometry and Bioanalyzer RNA 6000 nano kit. RNAseq was completed on each sampling using Illumina HiSeq 2000