mock bolting replicate 2
RNA was extracted from mock-inoculated and evolution passage five (aerial) infected plant tissue using NZY Total RNA Isolation Kit (NZYTech, Lisbon, Portugal).  The quality of the RNAs used to prepare RNA-seq libraries was checked with the Qubit RNA BR Assay Kit (Thermo Fisher, Waltham MA, USA).  SMART libraries, Illumina sequencing (paired end, 150 bp), and quality check of the mRNA-seq libraries were done by Novogene (UK) Co. Ltd.