Long-read sequencing of X. birchmanni populations
We extracted genomic DNA using the Promega Wizard HMW DNA extraction kit (Catalog #A2920, Madison, WI), following the manufacturers instructions. DNA was quantified on the Qubit fluorometer and evaluated for quality using the Nanodrop and Agilent 4150 TapeStation machine. Extracted DNA was sent to the University of Washington Long Reads Sequencing Center for PacBio library prep and sequencing on 1 SMRT cell.