RNAseq-pam68-1
Total RNA was isolated by using Trizol reagent kit (Invitrogen, USA).The mRNA was enriched by removing rRNA with the Illumina Ribo-Zero (PlantLeaf) kit (Illumina, USA) and fragmented into short fragments. First-strand and second-strand cDNA was synthesized and then purified using the QiaQuick RPC extraction kit (Qiagen, Netherlands). The synthesized cDNA were end repaired, poly(A) added, and ligated to Illumina sequencing adapters. (NEB, USA).