Genome samples of Chortoglyphus arcuatus
The samples were sheared using the Covaris G-tube, 1500 ng of the sheared and purified DNA was used as input for the library preparation using SMRTbell Express Template Prep Kit 2.0. During library preparation, the samples underwent DNA damage and end repair as well as barcode adapter ligation. To remove the unligated DNA fragments from the libraries, nuclease treatment was performed. The SMRT libraries were size selected then sequenced using the 30-hour movie time on the PacBio Sequel II.