RNA-seq of Zea mays: heat temperature and guvermectin treatment
For transcriptomic analysis, samples of roots were collected from the maize seedlings (strain B73) previously soaked in guvermectin or water under the described high temperature conditions, as well as the samples from roots that had room temperature treatments. Total RNA was isolated from the samples using Trizol reagent (Invitrogen) and RNA Clean-Up Kit-5 (Zymo Research, R1016), following the manufacturers instructions. The extracted mRNA was used to construct the cDNA library, and the library construction was sequenced on an Illumina Hiseq 4002 Platform (Lc-Bio Technologies, Hangzhou, China).