topic expression of a truncated NLR from wild Arachis enhances resistance against Fusarium oxysporum
Total RNA was extracted from three pools of five T2 seedlings roots of AsTIR19-OE-11 line and WT submitted to F. o. f.sp. conglutinans infection (6 DAI) and control using the TRIzol Reagent (Ambion, Foster City, CA, USA), and purified using the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany). The RNA integrity and quantity was verified using gel electrophoresis and 2100 Bioanalyzer system (Agilent, Santa Clara, CA, USA). Four cDNA libraries (AsTIR19-inoculated, AsTIR19-non-inoculated, Wild-inoculated, Wild-non-inoculated) were prepared with the TruSeq Stranded mRNA Library Prep Kit