Single cell RNA-Seq of a sample from OGC at the fourth passage
Single-cell suspensions with 90% cell viability were processed on the 10x Chromium Controller using Chromium Next GEM Single Cell 3 Reagent Kits v3.1 (10x Genomics) according to the manufacturers instructions. Cells were partitioned into nanoliter-scale Gel Beads-in-Emulsion (GEMs) with target recovery of 10,000 cells. The single-cell 3 prime mRNA seq library was generated by reverse transcription, cDNA amplification, fragmentation, and ligation with adaptors followed by sample index PCR. Resulting libraries were quality checked by Bioanalyzer and sequenced on an Illumina NovaSeq 6000 (index = 8 bases, read 1 = 26 bases, and read 2 = 91 bases).