Complete genome sequence of Lactobacillus plantarum MTCC 25432
Microbial genomic DNA was extacted by Qiagen DNA extraction Kit. Samples were treated with RNase (New England Biolabs, USA) to remove ribonucleic acids (RNAs) from the nucleic acid pools. Finally, genomic DNA was precipitated using 90% ethanol. The precipitated DNA was washed two times with 70% ethanol to remove salt and other contaminants. Quality and quantity of the DNA isolation from each of the samples were monitored by resolving the sample in 0.8% agarose gel. The 260/230 and 260/280 ratios were used as a secondary measure of genomic DNA purity. Sample indexing was done by Nextera XT Index Kit (Illumina) and quantification of DNA library was performed by Qubit Flurometer using Qubit dsDNA HS Assay Kit (Invitrogen) and amplicon length was checked using 2100 Bioanalyzer instrument. The final libraries were pooled and sequenced using HiSeq2500 platform following 2x250 paired-end chemistry