Analysis of CD16 polymorphisms
Genomic DNA was extracted from PBMCs using the QIAamp DNABlood mini-Kit according to the manufacturers instructions, and theregion which contains the polymorphic sequence was amplified by PCR,using the following primers: forward 5-CCCTTCACAAAGCTCTGCACT-3; reverse 5-ATTCTGGAGGCTGGTGCTACA-3; sequencing: 5-CCCCAAAAGAATGGACTGAA-3. The sequencing reaction wasperformed using the BigDye Terminator v3.1 Cycle Sequencing Kit anda SeqStudio Genetic Analyzer System. Sequences were finallyanalyzed with the Sequencing Software Analysis 7.