Metagenome of Anas platyrhynchos domesticus: cloacal swab
Viral RNA was reverse-transcribed into cDNA using random hexamers in a single step process. The resulting first strand cDNA was used as template to synthesise the second strand,  generating dscDNA which was used as a template to prepare Illumina sequencing library following Illumina DNA prep kit protocol. Indexed multiplexed samples were pooled and reconstituted to 4 nM before diluting to 12 pM for loading in to MiSeq instrument