the RNA-Seq of  the Shine Muscat  berry with NAA
In this project, total RNA of each sample was extracted from the Shine Muscat (Vitis labruscana Bailey  V. vinifera L.) fruit according to the instruction manual of the TRlzol Reagent (Life technologies, California, USA). RNA integrity and concentration were checked using an Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA, USA). In briefly, the enriched mRNA was fragmented into approximately 200nt RNA inserts, which were used to synthesize the first-strand cDNA and the second cDNA. The double-stranded cDNA were performed end-repair/dA-tail and adaptor ligation. The mRNA was amplified by NA nanosphere technology (DNB) for BGI Genomics. The cDNA library was identified and sequencing following the method of combined probe anchoring. Finally, the constructed cDNA libraries of the Shine Muscat (Vitis labruscana Bailey  V. vinifera L.) fruit were sequenced on a flow cell using an BGISEQ-68 platform.