Genomic sequencing of PSU5579
Genomic DNA was isolated using the Promega Wizard genomic DNA purification kit and resuspended in 2 mM Tris-HCl buffer (Bio Basic, Inc.). DNA was quantified using a NanoDrop 1000 spectrophotometer (ND-1000) and checked for quality on a 1% agarose gel stained with ethidium bromide. The DNA was sequenced on an Illumina MiSeq sequencer at the Genomics and Sequencing Center at University of Rhode Island.