RNA-seq of MHCC97H cell: knockdown 3
Total RNA was isolated from the cells using TRIzol reagent (Invitrogen, USA) according to the standard procedure. After checking the integrity of the RNA using an Agilent 2100 bioanalyzer, poly-A-containing mRNAs were enriched using oligo (dT)-attached magnetic beads and randomly cleaved with Fragmentation Buffer (NEB, USA). The cDNA library was then established by reverse transcription of fragmented mRNA and cDNA amplification by PCR.