Alteromonas gracilis strain J4, whole genome Nanopore Sequencing
Strain J4 was isolated from rhizoids of C. prolifera collected in Ria Formosa lagoon (3700'22.7"N 758'00.3"W, Faro, Portugal) by hand and stored in a cooling box. Rhizoid samples were manually ground with mortar and pestle, and the lysate was subsequently plated on Difco Marine Agar 2216 and incubated in the dark at room temperature (20-25C). After 3 days, individual colonies were selected, replated, and incubated in a similar fashion. Bacterial genomic DNA was extracted using the VWR peqGOLD Bacterial DNA Mini Kit (VWR), after which whole-genome DNA sequencing was conducted on a portable MinION Mk1C device (Oxford Nanopore Technologies), using the Ligation Sequencing Kit (SQK-LSK110) and a Flongle Flow Cell (R9.4.1).