CUT&Tag-Seq of mus musculus: macrophages sorted from tumor
Briefly, TAMs were sorted and centrifuged for 5min at 300g at room temperature. Cells were washed two times with 1.5mL wash buffer. After prepared by concanavalin A coated magnetic beads, the cells were incubated with primary antibody (H3K4me1 and H3K27ac), secondary antibody (Goat anti-Rabbit IgG H&L) and Hyperactive TN5 Transposon and then fragmented. The fragmented DNA extracted from the samples were amplified by PCR and the CUT&Tag libraries were constructed. Then, the libraries were sequenced with the Illumina HiSeq 3000 at Genergy Co., Ltd (Shanghai, China).