RNA-seq of CRFK cells infected with FHV-1 vaccine strain in vitro
TruSeq stranded mRNA library preparation kit (Illumina Inc., USA) was used to construct cDNA libraries and samples with the correct fragment size (~260bp) were normalised to 20 M and sequenced on the NextSeq 500 sequencing platform to produce libraries of 150 bp paired-end reads (Illumina Inc, USA).