ddRADseq of coffee genotype Catua, In_vitro_(M1V1) material treated with SodiumAzide_(5_mM_/_15_minutes)
BglII and DdeI digested genomic DNA in a 40 ul reaction mix for four hours at 37 C . The resulting DNA fragments were then purified using Agencourt AMPure XP beads according to the manufacturers instructions.