DRM1_2
C. lusitaniae strains were subcultured from overnight into 5 ml YPD cultures, and grown for 6 h at 37 C. Each strain was either grown for 6 hours with no addition of chelator, or with the addition of 80 M bathophenanthroline disulfonate iron chelator for the final hour of growth. At endpoint, all strains were spun down, and snap frozen in liquid nitrogen. RNA was isolated using Epicentre Yeast RNA isolation kit, and submitted to MiGS for sequencing.