ICPC Lung Cancer East Asia, Tumor RNA Sequencing: Sample P059-T-RNA
RNA was isolated from frozen tumor and adjacent normal tissues using AllPrep DNA/RNA/miRNA Universal Kit (Qiagen). RNA quality was confirmed using Bioanalyzer 2100 (Agilent Technologies) and Nanodrop 2000 spectrometer (Thermo Fisher). The library preparation of mRNA sequencing was performed on 1 μg of total RNA with DNase I treatment using TruSeq Stranded Total RNA Sample Preparation Kit (Illumina). The concentrations of all libraries were quantified by Qubit assays (Thermo Fisher) and the sizes of libraries were measured by Agilent TapeStation (Agilent). The paired-end sequencing was performed using HiSeq 4000 (Illumina).