Nanopore reads of Human astrovirus
Extracted RNA was subjected to the synthesis of cDNA using NEBNext Ultra II RNA First Strand Synthesis Module (New England Biolabs, USA) and NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (New England Biolabs, USA). cDNA aliquots (10 L) were mixed and enriched with AMPure XP beads (Beckman Coulter, A63881) by 1.8 volume content. A 15-L enriched cDNA was used for library preparation. For the MinION (Oxford Nanopore Technologies), we used the Rapid Barcoding Kit (SQK-RBK004) for library preparation and R9.4 flow cell (FLO-MIN106) for sequencing according to the manufacturer's protocol.