RNA-Seq of Arabidopsis thaliana: whole 5 day old AtWHIRLY1 knock-out seedling
mRNA was enriched from the total RNA by poly-A selection. After fragmentation, cDNA synthesis was synthesized using random hexamer primers. Overhangs were converted into blunt ends and then the 3' ends of DNA fragments were adenylated. Adaptors were ligated to the DNA fragments. Only 150-200 bp-long, adaptor-ligated cDNA fragments were used for PCR to create the sequencing library.