The complete chloroplast genome of Salsola pellucida
First, a DNAsecure Plant Kit (DP320 TIANGEN Biotech, Beijing, China) was used to extract total genomic DNA following its manufacturers instructions. The Genomic DNA was subsequently fragmented into approximately 300-500 bp using Covaris M220 Focused-ultrasonicator. Next, the NEBNext Ultra II DNA Kit was used for the assembly of the DNA library suitable for Next Generation Sequencing (NGS). The final phase involved sequencing the DNA library utilizing PE150 strategy (150-bp paired-end sequencing) on the DNBSEQ-T7 sequencing platform.