Piglet ileon digestate sample, block 1 ,5%BC treatment, day 22, high weigth, animal J174
DNA extraction was performed using the QIAamp DNA Stool Mini Kit (QIAGEN, Hilden, Germany) according to the manufacturers instruction. The DNA was extracted from digestates using a bead beating method (Zaheer et al., 2018) with minor modifications: frozen fecal sample was diluted with 0.9% saline (1:2) before processing, and the homogenization step was done in a Bead Ruptor at 4,000 rpm during 3 minutes. For all samples, the DNA integrity was verified by electrophoresis in 1% agarose gel. and quantified using the NanoDrop apparatus and the Quant-iT PicoGreen kit (Invitrogen)