HEK293T_TUT4/7_DKO_NEBnext
HEK293T cells were supplemented with 10% FBS (Life Science Group), 100 U/ml penicillin G, 100 g/ml streptomycin and 1x MEM non-essential amino acids (Thermo Fisher Scientific). Total RNA from cell lines was isolated using TRIzol reagent (Thermo Fisher Scientific) according to the manufacturers' protocol. Libraries were prepared using NEBNext Small RNA library Prep Seq for Illumina using 15 l for library preparation and then adjusted to equal concentrations after qPCR.