RNAseq of mus musculus: adult mid. Small intestine contents
Metagenomic libraries from DNA (26 ng) were prepared using the NEBNext Ultra II FS DNA Library Prep Kit (New England BioLabs, Ipswich, MA, USA) and the < 100 ng input protocol with 15 min of fragmentation (step 1.1.5), adapter ratio of 1:12 (step 1.2.1) and 6 cycles of PCR enrichment (step 1.4.3), generating fragments with an average size of 310  60 bp