Small RNA-Seq of Mus musculus
The sncRNA (<200nt) fractions from the germline, liver, and cortex tissues were subsequently used for library preparation using the NEBNext Multiplex Small RNA Library Prep Kit (NEB, #E7560S). Sequencing was performed on an Illumina platform on a 300 cycle S4 NovaSeq shared flow cell, using 150 bp paired-end reads, accounting for at least 37 million reads per sample