Abundance Assay
Myc-tdMCP-SNAP was co-expressed in cells stably expressing the library of Flag-MCP-tagged BRaf mutants and MS2-circRNA barcodes. Cells were treated with 4nM SJF0628 for 20hrs prior to lysis. After lysis, the sample was split and a subsequent flag-IP was performed on this sample. The co-enriched MS2-circRNA barcodes were extracted and sequenced. Corresponds to cell transduction and flag enrichment #2 (biological replicate #2).