RNA-Seq of Danio Rerio: pools of isolated larval head
Three replicate larvae from Het in-crosses, aged 5 dpf, were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies, which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20-25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer, and only samples with an RNA integrity number value of >5.8 were used for library preparation.