16S metabarcoding of archaeal community StB1
V3-V4 regions of 16S rRNA genes were amplified by PCR using primers for archaea Arch349F and Arch806F. The libraries were pooled by equal molarity. Denaturation and sample loading were performed according to the Illumina Sample Preparation Guide using a MiSeqReagent Kit v3, 600 cycles (Illumina, USA).