Sediment microbial community from Little Sippewissett Salt Marsh, MA, USA ; I5_Active_Subset
16S rRNA genes were amplified with 515F and 806R primers over 35 cycles of PCR. Following purification, eight more PCR cycles were used to attach barcodes and sequencing adapters. After a second purification, amplicons were sequenced with Illumina MiSeq 2x250.