GW Crispr-Cs9 KO in C57BL/6 CD4 T cells : Spleen of Balb/c mice
To systematically identify and benchmark targets enhancing T cell resistance to host immune rejection, we mutated a pool of primary CD4 T cells from C57BL/6 mice (H2-Kb haplotype)  at genome-wide level and subjected them to an in vivo allogeneic selection by injecting them into fully competent MHC-mismatched BALB/c mice (H2-Kd). We then compared gRNA composition in those cells to the same cells transfered to MHC-matched C57BL/6 mice in spleen and lymph nodes.