Custom probe ribosomal depletion RNA-seq of B. malayi
Total RNA was extracted from 3 B. malayi fresh frozen adult female worms. Ribosomal RNA was depleted using custom rRNA depletion probes designed against B. malayi and wBm ribosomal sequences. The RNA library was prepared using the NEBNext Ultra II Directional RNA-seq kit (E7765) with 10 PCR cycles.