PacBio Sequel II CLR of Pan troglodytes: adult male Chaos
The peripheral blood DNA samples were assessed as suitable for PacBio Sequel II sequencing. DNA was fragmented with the Covaris g-TUBE device. Next, DNA damages were repaired using the DNA Damage Repair reagents (PacBio). To ligate the hairpins (SMRTbell templates) to the DNA fragments, BLUNT hairpin adapters oligonucleotide pre-annealed stocks were used. To remove failed ligation products, exonuclease was added. Three-step AMPure PB Size-Selection and Purification was performed. Prior to sequencing, primer was annealed to both ends of the SMRTbell template. The binding reaction was performed and DNA sequencing polymerases were bound to the primer-annealed SMRTbell templates.