Short-read sequencing of Dharani genotype of vetiver
DNA was extracted from the plant leaf and was quality checked with Qubit dsDNA kit. DNA was fragmented and tagged with the Illumina bead-based transposome complesxTagment procedure using Illumina DNA Prep Library kit. Post the tagmentation cleanup, the fragements were indexed with adaptors and PCR enriched. The libraries were cleaned up using sample puriication beads and quantified using Quantus flurometer and distribution of library size was determined using Agilent Tapestation 4200.