Sample 2 (coronary sample with plaque erosion from a 46-year-old white female)
Raw FASTQ files for a given capture area and corresponding capture area histology images were processed with Space Ranger software version 1.3.0 (10X Genomics, Pleasanton CA). Space Ranger Pipeline was used to align Fastq reads to human GRCh38 reference genome, and the probe set reference file specific only to human FFPE tissues because the synthetic probe DNA was sequenced rather than cDNA of a captured. Sequencing data was then imported into Partek Flow Enterprise Edition software (Partek Inc., Louis, MO) for generatig PCA and UMAP.