16S rRNA gene Seq of Bufo melanostictus : Tadpoles gut
Total genomic DNA was extracted using the CTAB method. The V3V4 region of the bacterial 16S ribosomal RNA (rRNA) gene was amplified by the polymerase chain reaction (PCR) using the primers 338F: 5-ACTCCTACGGGAGGCAGCA-3 and 806R: 5-GGCTACHVGGGTWTCTAAT-3.