RNA-seq of equus caballus: adult male  skelental muscle
Total RNA from twelve GM muscles of Mongolian horses (N = 6/group) were extracted by using the Trizol reagent (Invitrogen, Carlsbad, USA), and purified with Kit, TRK1002 (LC Science, Houston, USA). RNA purity was checked by the NanoPhotometer spectrophotometer (IMPLEN, CA, USA). The Qubit RNA Assay Kit in Qubit2.0 Flurometer (Life Technologies, CA, USA) was used for measuring RNA concentration. RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Libraries of RNA sequencing were generated using the rRNA-depleted RNA by NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, USA) and sequenced on an Illumina Hiseq 2500 platform (150 bp paired-end reads were generated). Libraries of small RNA sequencing were generated using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB, USA) and on an Illumina Hiseq 2500/2000 platform (50bp single-end reads were generated).