Genomic sequence of Pantoea agglomerans AR8b: Illumina reads
High molecular weight genomic DNA was extracted (Miller et al. 1988) with the following modifications: the pre-lysozyme lysis buffer was 1M Tris pH 7.5, 0.5M EDTA pH 8.0, 5M NaCl, lysozyme digestion was performed at 48C for 1h, DNA resuspension was in 10 mM Tris pH 7.5, RNAse treatment was followed by two additional washes. DNA quantity was assessed using a Qubit 3.0 fluorometer (Thermo Fisher Scientific, U.S.A.). DNA integrity was assessed by agarose gel electrophoresis. For short-read sequencing, was prepared by the Cornell Genomics Facility, part of the Biotechnology Resource Center using the the DNA library Nextera DNA Flex Library Prep Kit (Illumina). The DNA library was sequenced on a MiSeq platform (Illumina, USA).