WGS HiFi sequencing of Rosa canina
HiFi library was prepared using SMRTbell Express Template Prep Kit 2.0 with an initial DNA fragmentation using Diagenode Megaruptor 3 and final library size binning into defined fractions by Blue Pippin with 0.75% agarose cassettes (Sage Science). Size distribution was controlled by Femto pulse system (Agilent, Santa Clara). Size-selected libraries were sequenced on a Sequel II device with Binding kit 2.0 and Sequel II Sequencing Kit 2.0 for 30 h using two SMRT cells.