Subject 10 Tumor RNA sample at timepoint = PRE, sequenced in batch 1
RNA was extracted from frozen tumor sections using the Qiagen RNA mini kit. Library preparation was performed by Psomagen (Brooklyn, NY, USA; formerly Macrogen) using the TruSeq Stranded Total RNA LT Sample Prep Kit (Human Mouse Rat) following the TruSeq Stranded Total RNA Sample Prep Guide (Part # 15031048 Rev. E). Psomagen then performed paired 2x150bp sequencing using NovaSeq6000 S4 v1.0 (300 cycles, Illumina).