RNA-Seq of infant gut microbiota
To explore whether the association between GDM and infant body mass index (BMI) in early childhood is affected by the meconium microbiota, we recruited 120 mothers (60 healthy women and 60 with GDM) and their newborns from the Womens Hospital of Nanjing Medical University. Meconium of 120 neonates was collected within few hours after birth and sequenced using 16S rRNA sequencing analysis. Childrens BMI was measured at 41 months old.