Wheat rhizosphere
Evaluation of the initial DNA concentrations in the prepared samples was carried out using the Life Technologies Qubit dsDNA HS Assay Kit produced by Carlsbad, USA. The samples were further cleaned with DNEasy PowerClean Pro Cleanup Kit (Qiagen) and the concentrations of the samples after the cleanup were again checked using the Qubit dsDNA HS Assay Kit (Life Technologies, the USA). The library was then prepared using 2025 ng of DNA, Nextera DNA library preparation Flex kit (Illumina) was employed in the library preparation, following standard procedure.