Chip-seq of Mus musculus: in vitro myoblasts
Libraries were prepared using the MicroPlex V2 librarypreparation Kit (Diagenode; Cat#: C05010012), followingthe manufacturers instructions, 10 l of DNA was used asstarting material. After end repair of the double-strandedDNA templates, cleavable stem-loop adaptors were ligated,and then adaptor enriched DNA was amplified with 10PCR cycles to add Illumina compatible indexes. The li-braries were then purified with the Agencourt AMPure XPbead-based purification system. Final libraries were quan-tified with Qubit dsDNA HS assay (Thermo Fisher; Cat#:Q32851), and size distribution was monitored by capillaryelectrophoresis on Agilent 2100 Bioanalyzer using DNAHigh Sensitivity HS Kit. Libraries were then normalizedto 10 pM and pooled before sequencing. Libraries weresequenced on an Illumina Nextseq 500 in a paired-endmode in two independent biological replicates at PlatformGENOMICInstitute Cochin.