RNA-seq of wild-simulated ginseng of 13yr leaf rep1
Seven- and thirteen-year-old wild-simulated ginseng samples were collected from cultivation sites monitored by Forest Medicinal Resources Research Center of National Institute of Forest Science in Korea. Total RNAs were extracted from 7- and 13-year-old wild-simulated ginseng samples using cetyltrimethylammonium bromide and sodium dodecyl sulfate-lithium chloride methods (Vennapusa et al., 2020). Their quality and quantity were examined using a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Two-microgram total RNAs were used to construct RNA-Seq libraries using an Illumina TruSeq RNA Sample Prep Kit according to the manufacturers instructions. Pooled libraries were sequenced using an Illumina HiSeq X platform with paired-end (PE) reads of 151 bp