Seq individual virome of child E1: NP swab
Nasal and throat swabs from children with respiratory symptoms were initially diagnosed using qPCR major respiratory pathogen kits. The SISPA approach was used for the metagenomic study. DNA/RNA were extracted by column-based extraction kit (Biolabmix); cDNA synthesis was performed using M-MuLV-RH reverse transcriptase (Biolabmix) and Klenow fragment (SibEnzyme). dsDNA was purified with Agencourt AMPure XP magnetic beads (Beckman Coulter); amplification was performed with polymerase mix Encyclo Plus PCR (Evrogen); and the amplification products were purified using a kit for DNA extraction from reaction mixtures (Biolabmix). The KAPA Hyper prep kit (Roche) was used to prepare the libraries. Sequencing was carried out using the Genolab M platform (Genemind).