Infant ID:1100:Adjusted Age:31.57
DNA samples extracted from stool were quantified using an Agilent 4200 Tapestation instrument, with corresponding Agilent Genomic DNA ScreenTape assays. The resulting DIN (DNA Integrity Number) scores and concentrations were taken into account for qualifying samples to proceed. The samples were then normalized to 5ng/uL, before amplification of the V3V4 region occurred. A cleanup of the amplicons was performed using Aline PCRClean DX beads, followed by indexing using indexed-adapters from the Nextera XT Index Kit. A cleanup of the final libraries was performed using Aline PCRClean DX beads. The resulting purified libraries were pooled in equimolar ratio and sequenced on an Illumina MiSeq instrument in paired-end 300 bp mode.