SD117369_contaminated_spikeinExperiment
Genomic DNA was extracted using the DNeasy UltraClean 96 Microbial Kit (Qiagen Cat. #10196-4) following manufacturers instructions. The 16S V4 region was amplified using 515F and 806rB primers using Platinum II HotStart PCR Master Mix (ThermoFisher Cat. #14000013). The PCR products were pooled at equal volume and gel-purified. Final purification was performed using Macherey-Nagel NucleoSpin Gel and PCR Clean-up, Mini Kit (Fisher, Cat. #740609). Amplicons were sequenced on the Illumina MiSeq platform with 250-bp paired reads at the Stanford Chan Zuckerberg Biohub Facility.