RNA-Seq of PER71: mycelia grown on rubber wood block for 47 days
Total RNA was extracted and purified. Using SMART cDNA synthesis ds-cDNA was synthesized. Next, cDNA and amplification were performed, and cDNA was fragmented. AMPure SPRI bead selection was performed for the removal of small fragments. Then the cDNA blunt end was polished and A tailing was added to the 3 end. The fragments were ligated with adaptors and PCR amplification was done. Subsequently, size selection was performed via gel and AMPure SPRI beads again. The final sample was quantified and sent for sequencing.