NEBNext Human/Mouse/Rat/Bacteria treated RNA-seq of B. malayi
Total RNA was extracted from 3 B. malayi fresh frozen adult female worms. Ribosomal RNA was depleted using the NEBNext Human/Mouse/Rat rRNA depletion kit (E7400) with the NEBNext Bacterial rRNA depletion probes (E7850) added in. The RNA library was prepared using the NEBNext Ultra II Directional RNA-seq kit (E7765) with 10 PCR cycles.