RNAseq of mus musculus: adult Colon contents
Metagenomic libraries from DNA (26 ng) were prepared using the NEBNext Ultra II FS DNA Library Prep Kit (New England BioLabs, Ipswich, MA, USA) and the < 100 ng input protocol with 15 min of fragmentation (step 1.1.5), adapter ratio of 1:12 (step 1.2.1) and 6 cycles of PCR enrichment (step 1.4.3), generating fragments with an average size of 310  60 bp