16S rRNA of JNR water
The V3- V4 region of domain bacteria 16S rRNA amplicon library was generated using NEBNext Ultra II DNA Library prep ket for Illumina (new England biolabs, USA) and NEVNEX multiplex oligos for Illumina. The V3-V4 region of the 16S rRNA was amplified using the primers were the V3-V4F357 (3CCTACGGGNGGCWGCAG5) and V3-V4R805 (3GACTACHVGGGTATCTAATCC5).