spatial RNAseq of human ovary
Ovary tissues were cut into about 10*10*5 mm piece and dried with lab blotting paper to prevent ice crystal formation. After that we embedded the tissue with opti-mum cutting temperature compound into the embedding box in dry ice and saved in -80 . Human ovarian samples were then processed for full ST experiment as the manufacturers instructions (13x Genomics, Visium Spatial, Rev B).