Genomic sequence of Pantoea agglomerans ROTS050421: Nanopore reads
High molecular weight genome DNA was extracted using Puregene Yeast/Bact. Kit B (Qiagen, Germany), Protocol for DNA Purification from Gram-Negative Bacteria, except DNA was resuspended in 10 mM Tris pH 7.5 instead of DNA Hydration Solution. The isolated DNA was used to prepare a Nanopore sequencing library using the ONT Rapid Barcoding Sequencing kit (SQK-RBK004). The sequencing reaction was run in a MinION flow cell. Base calling was performed using Guppy v. 6.0.1 and de-multiplexed with QCAT.