Single Cell RNAseq of Macaca Mulatta: adult bronchial alveolar lavages
Freshly thawed BAL leukocytes from SVV infected animals at DPC 0, 3, 7, and 14 were resuspended to a final concentration of 1500 cells/uL. Single cell suspensions were then immediately loaded on the Chromium Controller (10X Genomics) with a target of 20,000 cells. V3 chemistry was used for library generation, before being sequenced on an Illumina NovaSeq 6000 with a sequencing target of 30,000 paired reads per cell.