Genome assembly of Neopusillimonas maritima 17-4A
Genomic DNA was extracted from fresh leaf tissue using the CTAB Method. DNA quality and concentration were assessed using a NanoDrop spectrophotometer and Qubit fluorometer (Thermo Fisher Scientific). Approximately 1 g of DNA was fragmented to an average size of 300 bp using a Covaris S2 sonicator. Fragmented DNA was end-repaired, A-tailed, and ligated to Illumina-compatible adapters using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs). The ligated products were purified using AMPure XP beads (Beckman Coulter) and amplified via PCR with 10 cycles. Library size distribution was confirmed on an Agilent 2100 Bioanalyzer. Sequencing was performed on an Illumina NovaSeq 6000 platform using a 150 bp paired-end configuration.