poolseq of cyclical parthenogenetic pea aphid females collected on pisum sativum
DNA was extracted from one adult parthenogenetic female per lineage using the Qiagen DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany) following the manufacturer instructions. After RNAse treatment, DNA solutions were pooled in equimolar proportions for each population ensuring that each lineage contributed equivalent amounts of DNA to the pool. Paired-end 150 bp libraries were constructed per population from these DNA pools using the Genomic DNA Sample Preparation Kit (Illumina, San Diego, CA). The resulting libraries were sequenced on Illumina NovaSeq using S4 flowcells chemistry at the GetPlage Platform of Genotool-Toulouse.