DNA-Seq of respiratory sample
Genomic DNA libraries were constructed using the NEBNextR Ultra DNA Library Prep Kit (NEB. USA) according to the manufacturer instructions (Illumina, 2022). Libraries were analyzed for size distribution by an Agilent2100 Bioanalyzer and quantified using real-time polymerase chain reaction. DNA libraries were then sequenced on an Illumina NovaSeq 6000 instrument according to standard protocols (Illumina, 2020).