Bacterial communities in Picea abies rhizosphere in the Czech Republic
Sixteen soil cores (4 cm diameter) were used to sample all material up to the bottom of the soil organic matter at a defined area of 3 x 3 m. . Bacterial communities were characterised by amplifying the V4 region of the 16S rRNA using primers 515F and 806R. Amplicons were sequenced on a Illumina Miseq using 2 x 250 bp chemistry.