Characterizing the Invasion of Water Primrose in the California Bay-Delta
Fresh frozen tissue was ground using a micropestle and extracted using a Qiagen DNAeasy PlantPro kit. Libraries were prepared using the BEST protocol (Care et al., 2017), before using the Angiosperms353 Target Capture Kit (Johnson et al., 2018; https://doi.org/10.1093/sysbio/syy086) to isolate and amplify genes indicative of within-species biodiversity (Slimp et al., 2021; https://doi.org/10.1002/aps3.11419). Libraries were sequenced on a Nextseq550 to a read length of 75.