RNA-Seq of equine wound dressing: D14 cntrol
Evaluation of wound microbiome was performed on samples of exudate soaked wound dressings. For this a 4 mm leather punch was used to remove two identical disks of dressing material from the most representative area at the time of bandage change on Day 7 (pre-treatment), 14, 20 and 28. For each collection process a decontaminated punch and work surface was used. For decontamination, utensils underwent a detergent wash and ethanol rinse. The duration of sample handling (from dressing removal to placement of duplicate samples into an ethanol-dry ice slurry) was recorded to ensure samples were handled in a consistent manner. Samples were stored at -80oC for later microbiome analysis using standard RNA extraction and next generation sequencing methods (Australian Genome Research Facility, Melbourne, Victoria, Australia)