RNA-seq of coculture of V.dispar and L. acidophilus during the ES phase, B2
Bacteria, V. dispar and L. acidophilus, were harvested during specific growth phases and then centrifuged. Total RNA was extracted using the Presto mini RNA bacteria kit. RNA concentration was measured with a Qubit fluorometer, while its purity and integrity were verified using a NanoPhotometer and an Agilent FA5200. For RNA sequencing, cDNA libraries were made using the Illumina Ribo-Zero Plus kit and sequenced on a Novaseq 6000 platform by Genomics Co. in Taiwan. The data was cleaned using a Phred score threshold of 30.