Rna-seq of Hyptiotes cavatus: adult female cribellar gland
RNA was extracted from six H. cavatus individuals to create three replicates , for each of four tissue types: total silk glands, cephalothorax, cribellate and major ampullate. The cribellate and major ampullate gland replicates were created from the same three individuals, while the cephalothorax and total silk replicates originated from the remaining three. RNA was extracted using the PureLink RNA Mini kit with on-column DNAse treatment (TthermoFisher Scientific) and quantification was done with a Qubit Fluorometer. All samples were sent to Novogene for library preparation and sequencing. Each library was paired end 150 bp sequenced on an Iillumina HiSeq System.