MRP_200ug/ml_1
A.baumannii AB5075 was grown in RPMI+10%LB in biological duplicates. At A600  0.4, samples were treated with 200 g/ml, 100 g/ml, 25 g/ml, and 0 g/ml Meropenem for 15 minutes. 3 ml of cell broth was immediately added to two volumes of Qiagen RNA-protect Bacteria Reagent (6 ml), vortexed for 5 s, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 r.p.m. The supernatant was decanted, and total RNA was isolated from the cell pellet using the Zymo Research Quick-RNA MicroPrep Kit as per vendor protocol. An on-column DNase treatment was performed for 30 min at room temperature. The ribosomal RNA was removed using anti-rRNA DNA Oligo mix and Hybridase Thermostable RNase H (Biosearch Technologies, H39500). A Swift RNA Library Kit was used following the manufacturers protocol to create sequencing libraries.