Barcode Sequence Missassociation
Two cell lines stably expressing orthogonal libraries of MS2-circRNA barcodes and either Flag-tdMCP-BRaf wild type or Myc-tdMCP-BRaf wild type were mixed, lysed, and subjected to Flag-immunoprecipitations. Enrichment samples were collected 45 min after cell lysis. The co-enriched MS2-circRNA barcodes were extracted and sequenced.