RNA-Seq of Oryza sativa- 21 day old Root Control
21 days-old rice seedings were either kept in control conditions or subjected to 200 mM NaCl for 3 days and subsequently, shoot and root samples were separately harvested. Total RNA was extracted and samples with RNA Integrity Number(RIN)>7 were selected for library construction. mRNA was purified using TruSeq RNA Sample Preparation Kit, Illumina and fragmented using divalent cations. cDNA was prepared using Superscript Reverse transcriptase II (Life Technologies) and second strand cDNA was synthesized. Random fragmentation of cDNA was accompanied by end healing, followed by 5' and 3' adapter ligation. The prepared cDNA library was amplified using PCR for the enrichment of the adapter-ligated fragments. The individual libraries were quantified using a NanoDrop spectrophotometer (Thermo Scientific) and validated for quality with a Bioanalyzer (Agilent Technologies). Adapter-ligated fragments were then PCR amplified and gel purified.