Sequencing of the 16S rRNA gene amplicon from mock community (ATCC 20 strain even mix)
DNA from the mock community was used  to construct the 16S rRNA gene library (V4 16S rRNA gene) following the protocol from the Schloss lab. The amplified DNA was normalized using the SequalPrep Normalization Plate (Invitrogen) and pooled to form a single library. The 16S rRNA gene amplicons were sequenced using Ilumina MiSeq (2x250, paired-end).