RNA-seq of CRFK cells infected with FCV field strain in vitro
TruSeq stranded mRNA library preparation kit (Illumina Inc., USA) was used to construct cDNA libraries and samples with the correct fragment size (~260bp) were normalised to 20 M and sequenced on the NextSeq 500 sequencing platform to produce libraries of 150 bp paired-end reads (Illumina Inc, USA).