Nanopore genome sequencing of Armadillidium arcangelii individual BXR23-T01
Six daughters of a female individual infected by Wolbachia were dissected, and their ovaries, nerve chains and digestive tracts were pooled and grinded in liquid nitrogen. DNA extraction from ground tissues was carried out with the Monarch HMW DNA Extraction Kit for Tissue (New England Biosciences). Two libraries were prepared using the Ligation Sequencing Kit V14 (SQK-LSK114, Oxford Nanopore Technologies) using the LFB buffer. DNA Control Sample (DNA CS) was added during preparation. Libraries were sequenced with an R10.4.1 flow cell on a MinION Mk1C (running MinKNOW 22.10.7). Reads were basecalled with Dorado 0.5.3 with the dna_r10.4.1_e8.2_400bps_hac@v4.1.0 model.