NPS of Patient 15 at T0
Nucleic acid extraction was performed by QiaSymphony automatic extractor (QIAGEN, Hilden, Germany) using DSP Virus/Pathogen Kit (QIAGEN) starting from 600 ul and eluting in 70 ul of AVE buffer. Sequencing libraries were prepared by using Ion AmpliSeq SARS-CoV-2 Insight Research Assay.