Metagenome sequencing of warth-like lesion on common crane
Sample for virus isolation was collected from a common crane (Grus grus) with warth-like lesions on the head. Tissue sample was homogenized in PBS by adding stainless steel beads and running 15 min at 50 Hz on TissueLyzer LT (Qiagen, Hilden, Germany). Then, sample was centrifuged at 10.000x g for 5 min. Total DNA was purified directly from tissue lysates using DNeasy Blood & Tissue Kit (Qiagen). Libraries for next-generation sequencing were prepared with a Nextera XT DNA Sample Preparation Kit (Illumina, San Diego, CA, USA) and subjected to sequencing on the Illumina NextSeq 500 platform.