Barcode Sequence Missassociation
Two cell lines stably expressing orthogonal libraries of MS2-circRNA barcodes and either Flag-tdMCP-BRaf wild type or Myc-tdMCP-BRaf wild type were mixed and lysed. Total RNA was extracted from the lysate and barcode sequences encoded on MS2-circRNA barcodes were amplified and sequenced. This lysate was used in the barcode sequence missassociation experiment.