MaseteITS15
The amplicons were diluted to a concentration of 4 nM using 10 mM Tris pH 8.5. Following dilution, the libraries were pooled for a single MiSeq run, with each library contributing 5 L and bearing unique indices. Pooled PCR amplicons were then purified and indexed using Nextera XT primers from Illumina Inc., CA, USA. Subsequently, the amplicon library was quantified, normalized, and pooled in preparation for loading onto the MiSeq flow cell for a 2300 paired-end sequencing. The resultant sequence reads were de-multiplexed using the on-system MiSeq reporter software.