RNA-seq of human myoblast cells
RNA libraries were prepared following the Illumina Stranded Total RNA library prep kit with a ribosomal removal step. Following library preparation, the concentration of each library was determined with a Qubit high sense DNA assay, and library quality was determined by the Agilent Fragment Analyzer. Final libraries were then pooled to 10 nM, and the concentration of the pool was determined using the Kapa Biosystems Universal qPCR kit (Roche, Wilmington, MA). After dilution to 300 pM and denaturing with NaOH, the pooled library was loaded onto a NovaSeq6000 SP flow cell (PE50; Illumina, San Diego, CA) for sequencing.