Mitochondrial genome sequence analysis of sea cucumber Apostichopus japonicus in Pingtan County Waters, Fujian province
Using the G-tubes method, genomic DNA is processed into 15-20k fragments, with flattened ends and circular single stranded ends connected to each other. The single stranded end is connected to the double stranded positive and negative ends, resulting in a dumbbell like structure called SMRT Bell. The library single stranded loop is annealed and bound to the polymerase at the bottom of the fixed ZMW (zero mode waveguide holes). Once combined, it can be sequenced on the machine