NOVA1ar/ar NT
We analysed single-cell reads obtained from 60-day-old organoids. We performed scRNA seq in 6libraries NOVA1hu/hu (NT, 10 M, and 30 M) and the NOVA1ar/ar (NT, 10 M, and 30 M).The organoids were dissociated to a single cell using Accutase (Thermo Fisher Scientific) and Papain/DNase (Worthington) and mechanical dissociation using a 1000 uL pipette. Afterdissociation, the cells were counted, and the viability was analysed. We dissociated the cellsfor 50 min, and every ten min, we mechanically dissociated the cells until we had only singlecells in suspension. The protocol of the scRNA sequence was executed by the UniversalSequencing Technology Corporation (Carlsbad, CA).