RNA-Seq of Uninfected Midgut of G. fuscipes - 005
Total RNA from individual G. fuscipes was extracted, infection status was determined by amplification of specific Tryp gene, Tubulin. After infection status was confirmed, RNA from three midguts were pooled for one biological sample, and a total of six biological samples were sequenced from uninfected and infected samples. Total RNA from each group were sent to Yale University Center of Genome Analysis (YCGA) for RNA-sequencing libraries preparation for mRNA and miRNA. All samples were sequenced on Illumina HiSeq or MiSeq platforms.