RNA-Seq of T. turgidum ssp. dicoccum cultivar Lucanica: 28 DAS leaf in Optimal N
RNA samples were processed using TruSeq RNA sample preparation kits v2 (Illumina, San Diego, CA, USA). Libraries were quantified by real-time PCR using the KAPA Library Quantification Kit for Illumina Sequencing Platforms (Roche, Basel, Switzerland), followed by quality control using a DNA 100 Chip on a Bioanalyzer 2100. Cluster generation and sequencing were carried out using the Illumina HiSeq 2000 instrument and TruSeq PE Cluster Kit v3, following the Illumina PE_Amp_Lin_Block_V8.0 recipe, and Illumina TruSeq PE Cluster v3-cBot-HS kits with the 2  100 cycles, paired-end, indexed protocol, respectively.