Chloroplast genome of Micranthes dungbooi plastome
After the DNA samples were tested and qualified, they were randomly interrupted using a Covaris ultrasonic crusher, and then the whole library preparation was completed by the steps of end repair, addition of A-tail, addition of sequencing junction, purification and PCR amplification. After the library construction was completed, Qubit 2.0 was used for preliminary quantification to dilute the library, and then Agilent 2100 was used to detect the insertion fragments of the library, and after the size of the insertion fragments conformed to the expected size, the effective concentration of the library was accurately quantified using the Q-PCR method to ensure the quality of the library.