RNA-Seq
The total RNA was extracted from 3 paraffin-embedded sections, each 5 micrometers thick, and 500 ng of total RNA was used to prepare the sequencign library, following the RNASeq RiboZero - Illumina Stranded Prep protocol (Ligation RiboZero, Illumina cat no 20040525). The sequencing parameters were set to 2x75 nt, with a read depth of 50 million reads.