RNA-seq of largemouth bass :kidney
This study aims to analyze the transcriptomic changes in largemouth bass at different time points following LMBV infection. We collected head kidney samples at 1, 4, 7, and 48 days post-infection and extracted total RNA. mRNA was enriched using Oligo-dT beads to investigate the changes in gene expression post-infection. By comparing control groups (injected with saline) and treatment groups (injected with LMBV), we aim to reveal how LMBV affects the host's immune response and viral biological processes. Data analysis will employ RNA-Seq technology to identify differentially expressed genes, enhancing our understanding of the mechanisms of LMBV infection.