Hyb-seq of Cardamine acris subsp. acris acraC149_8
The Hyb-Seq libraries were prepared using the NEBNext UltraTM DNA Library Prep Kit for Illumina (New England Biolabs, MA, USA), as described in detail in lenker & al. (2021). Cardamine-specific probes, developed in Melichrkov & al. (2020), capturing 2246 exons from 1235 genes, were used for target enrichment with the MYbaits kit (Arbor Biosciences, MI, USA). The target-enriched libraries were pooled with unenriched library aliquots in a 2:1 ratio. The final libraries were sequenced with 150 bp paired-end reads on an Illumina MiSeq system at BIOCEV, Czechia.