Priestia filamentosa H146 raw sequencing data
Genomic DNA of H146 strain were sheared using g-Tubes (Covaris) and concentrated with AMPure PB magnetic beads. Each SMRT bell library was constructed using the SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences). The constructed libraries were size-selected on a BluePippinTM system (Sage Science) for 30kb molecules, followed by the primer annealing and the binding of SMRT bell templates to polymerases with the Sequel II Binding and Internal Control Kit 1.0 Kit (Pacific Biosciences). Sequencing was carried out on the Pacific Bioscience Sequel II platform with Sequel II Sequencing 1.0 Bundle (Pacific Biosciences) at Novogene Co., Ltd. in Beijing,China.Genomic DNA of H146 strain was used to prepare a standard Illumina library, with an insert size to about 350bp, following the protocol and using standard Illumina adapter sequences. The library was sequenced at Novogene Co., Ltd. in Beijing,China., using an Illumina NovaSeq PE150 instrument (Illumina, Inc., San Diego, CA, USA) (www.illumina.com) to generate paired-end reads.