SRVP19_Freyarchaeia-1
Library preparation was performed using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina. Final sequencing-ready libraries were visualized using the Agilent 2100 Bioanalyzer before pooling, and the final pooled library was quantified through qPCR using the KAPA Library Quantification Kit for Illumina. Sequencing was performed using Illumina NextSeq 1000/2000 150 paired-end sequencing lanes with 10% PhiX spike-in controls. Post-sequencing bcl files were converted to demultiplexed fastq files per the original sample count with Illuminas bcl2fastq v2.20 software.