RNA-Seq of mus musculus: adult female spinal cord
Samples containing total RNA were processed with an Illumina TruSeq Stranded mRNA Library Prep Kit (Illumina Inc., San Diego, CA, USA) to create cDNA libraries. Following bead-based purification of the poly-A mRNA this fraction was used for RNA fragmentation, first- and second-strand cDNA synthesis, end repair, adapter ligation, PCR amplification and subsequent PCR product purification. All library preparation steps were performed in accordance with the manufacturers instructions.