RNA-Seq of mus musculus: male kidney
The total RNA of renal tissues was extracted separately using Trizol Reagent (Invitrogen, CA, USA) following the manufacturers procedure. The total RNA concentration, purity, and integrity were analyzed by Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and Agilent Technologies 2100 Bioanalyzer (CA, USA), respectively.