RNA-seq of 3 tissues from juveniles X. birchmanni.
We extracted total RNA with the Qiagen RNAeasy Mini Kit (Catalog #74106, Qiagen, Valencia, CA) and sent RNA to Admera Health Services for library preparation using the NEBNext Ultra II Directional library prep kit with Poly A Selection. Extracted RNA was sent to Admera Health Services, South Plainfield, NJ for library preparation and sequencing on Illumina HiSeq 4000. Extractions and library preps were paired between groups (e.g. at least one false gravid and non-false gravid replicate for each extraction and library prep batch) to control for batch effects in statistical analysis downstream.