RNA seq of human iPSCs with SART3 variant
The three iPSC lines (PCS-201-010 unedited control; PCS-201-010 heterozygous SART3 p.Arg836Gln variant or PCS-201-010 homozygous SART3 p.Arg836Gln variant) were grown up from frozen stocks three independent times to create triplicate cell pools for RNA sequencing. RNA was extracted using the ReliaPrep RNA Miniprep System (Promega, #Z6011), following manufacturers instructions. RNA Sequencing was carried out at the Victorian Clinical Genetics Services (VCGS), with Illumina stranded mRNA library prep and sequencing of paired 150 base-pair paired end reads and 30 million reads per sample on the Illumina Novaseq6000.