16S-NGS of aquifer microbiota
To generate 16S rRNA gene libraries, the V3V4 hypervariable region of the 16S gene was amplified using the 341F/806R primers containing CS1 and CS2 linkers. 1st and 2nd PCR were performed (barcoding Fluidigm). Sequences were obtained on the Illumina MiSeq platform in a 2  250 bp paired-end run (HyLabs Israel).