Genome Sequence of BTV7: OBP Live Attenuated Virus
Total RNA was extracted using Tri Reagent (LS) (Sigma) as described by the manufacturer. Self-annealing primers were used for cDNA synthesis and full-length amplification of cDNA was performed. Libraries were prepared using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs) and size selection completed using AMPure XP Beads (Beckman Coulter). Sequencing was performed on the Illumina MiSeq platform using the MiSeq Reagent kit v3 (600 Cycles) according to the manufacturer's instructions.