Sediment metagenome
Environmental DNA was purified from the composite sediment samples using the DNeasy PowerSoil Pro Kit (Qiagen, Germany), following the manufacturer's instructions. DNA libraries were prepared using the Nextera XT DNA Library Preparation Kit (Illumina Inc., San Diego, CA, United States) and IDT Unique Dual Indexes Tagmentation Kit (Illumina Inc., San Diego, CA, United States) with total DNA input of 1ng, before sequencing on on an Illumina NextSeq 2000 platform 2x150bp