WES of germline DNA extracted from peripheral blood
WES was performed from germline double-stranded DNA extracted from peripheral blood using the Qiagen AllPrep DNA/RNA Mini Kit on an Illumina HiSeq 4000 instrument. Library construction was performed using the KAPA Library Prep Kit with palindromic forked adapters from Integrated DNA Technologies and pooled before hybridization. Hybridization and M49capture were performed using the relevant components of Illumina's Rapid Capture Enrichment Kit, with a 37-Mb target.