HEK293T_TUT4/7_DKO_IsoSeek
HEK293T cells were supplemented with 10% FBS (Life Science Group), 100 U/ml penicillin G, 100 g/ml streptomycin and 1x MEM non-essential amino acids (Thermo Fisher Scientific). Total RNA from cell lines was isolated using TRIzol reagent (Thermo Fisher Scientific) according to the manufacturers' protocol. Libraries were prepared using IsoSeek protocol (https://www.biorxiv.org/content/10.1101/2021.05.04.442244v1)