Evolutionary Replicate of VAN resistance 15
Glycerol stocks of frozen ancestral and evolved-population samples were grown overnight in TSB with 1.5 mcg/mL vancomycin. We centrifuged the overnight cultures at 8,000 rpm for 3 minutes and removed the supernatant from the bacterial pellets. SeqCenter (Pittsburgh, PA) prepared sample libraries from these pellets using the Illumina DNA Prep kit and IDT 10bp UDI indices and sequenced them on an Illumina NextSeq 2000. Demultiplexing, quality control, and adapter trimming were performed with bcl-convert (v3.9.3)