Microbial community composition of Boddi-szek (South) soda pan water collected on 21 May 2021
A part of the 16S rRNA gene was amplified with fusion primers containing Bacteria-specific sequences Bakt_341F (5'-CCT ACG GGN GGC WGC AG-3', Herlemann et al. 2011 ISME J) and modified Bakt_805R (5'-GAC TAC NVG GGT ATC TAA TCC-3', Herlemann et al. 2011 ISME J; Takahashi et al. 2014 Plos One) and part of the eukaryotic 18S rRNA gene was amplified with fusion primers 574*F (5'-CGGTAAYTCCAGCTCYAV-3') and 1132R (5'-CCGTCAATTHCTTYAART-3') (Hugerth et al. 2014 Plos One) with Fluidigm CS1 and CS2 universal sequences at their 5' ends. The resulting PCR products were mixed before sequencing in 1:1 ratio.