WGS of human Stool: internal controls only
DNA was extracted from human stool using the Zymo quick-DNA fecal/soil DNA with 40-minute bead-bashing, and eluted into 1x TE buffer. An aliquot was set aside as a control. Internal controls (ICs) of A. hydrophila and L. pneumophila DNA (NIST RM 8376) were spiked-in at ~1e5 genome copy/uL and 1.4e4 genome copy/uL, respectively. A control aliquot was taken to check background signal and potential contamination from the IC addition. Separate dilution series were prepared for E. coli O157:H7 DNA (RM 8376), E. coli O104:H4 DNA (RM 8376), V. furnissii (RM 8376), and E. coli K12 DNA, starting at 1:10 dilution of the neat reference material into extracted stool DNA and making 6 total dilutions each (~1e6 copy/uL to ~10 copy/uL. NEBNext Ultra II FS was used to generate the libraries with NEB unique dual indices plate.