DNA was extracted from the soil samples using the Qiagen PowerSoil DNA Isolation Kit, and the analysis was carried out with 16S primers targeting the V4 hypervariable region of the 16S small-subunit (SSU). Amplicons were sequenced on an Illumina MiSeq using the 500 cycle MiSeq Reagent Kit v2 according to the standard manufacturer instructions.
WA-Omics_L.A.1.0 16S - Time point 10 min. after soil rewetting, replicate 4