RNA-seq of human:  male bone marrow
Mononuclear cells (MNCs) were isolated from BM aspirates (35 ml) of FA patients and HDs by density gradient centrifugation and plated in non-coated 75175 cm 2 tissue culture flasks at a density of 500 000/ cm2 in complete culture medium. MSCs were harvested, after reaching 80% confluence, using Trypsin, and were propagated at 5000 cells/cm2. For the study of the transcriptome, the cell fraction of the MSC cultures from P6-P8 was used. Total ribonucleic acid (RNA) from MSCs was extracted using Qiagen&#39;s RNeasy Mini Kit, according to the manufacturers instructions. The total RNA that had a standard of concentration 200 ng/mL, mass 10 mg and RNA integrity number (RIN) 8.0 was subjected to RNA-Seq. Sequencing was performed at the Massive Sequencing Unit of the Madrid Science Park (NIMgenetics). The analysis was carried out by the Bioinformatics service