16S rRNA gene seq. of MMTV-TGF-alpha C57BL/6: baseline
Stool DNA was isolated from ceacal samples using QIAamp DNA stool MiniKit (QIAGEN). V3-V4 regions of the 16S rRNA gene was amplified using KAPA HiFi HS Mix (Roche). Amplicons were indexed with dual indexes by Nextera XT Index Kit v2 Set-A (Illumina). The final pool library was prepared as equimolar concentrations of each sample and diluted to 35 pM final concentration, including 5% PhiX DNA as a positive control.