Nanopore_UMI_clonal_reads_Mock_community
Libraries for nanopore sequencing was prepared using the samples amplified with primers containing UMIs and the Amplicons by Ligation kit (SQK-LSK110, Oxford Nanopore Technologies) according to manufacturer's protocol. All samples were sequences individually on MinION R9.4.1 flow cells on a GridION. However, different versions of the MinKNOW software and Guppy were used as the samples were not sequenced at the same time. MinKNOW v.21.11.7 was used for the Askov soil samples (UMI and UMI_clonal) and basecalling was done with Guppy v.5.1.13 in super-accurate mode (dna_r9.4.1_450bps_sup.cfg). MinKNOW v.21.06.13 was used for the mock community UMI library and basecalling was done with Guppy v5.0.16 in super-accurate mode (dna_r9.4.1_450bps_sup.cfg). MinKNOW v.22.07.4 was used or the mock community UMI_clonal library and basecalling done with Guppy v6.3.1 in super-accurate mode (dna_r9.4.1_450bps_sup.cfg).