Barcode Sequence Missassociation
Two cell lines stably expressing orthogonal libraries of MS2-circRNA barcodes and either Flag-tdMCP-BRaf wild type or Myc-tdMCP-BRaf wild type were mixed, lysed, and subjected to Myc-immunoprecipitations. Dummy MS2-circRNA was included in the lysis buffer. Enrichment samples were collected 105 min after cell lysis. The co-enriched MS2-circRNA barcodes were extracted and sequenced.