GSM7635112: CD11b+ cells, LPS-treated PA14, Replicate 2, scRNA-seq; Mus musculus; RNA-Seq
CD11b+ cells from lung single cell suspensions were purified using anti-CD11b magnetic microbeads, then passed through a 40-µM cell strainer and enumerated immediately before loading onto a 10x Chromium controller for cell capture (targeting 5000 cells per sample) using the Chromium Single Cell Immune Profiling v2- Dual index kits. Libraries for gene expression were constructed following protocols from 10x Genomics. Final libraries were QCed by Agilent TapeStation and then sequenced using Illumina's Novaseq 6000 targeting 50,000 reads per cell.