GSM8164149: MRK-740_n=1_Input; Homo sapiens; ChIP-Seq
After harvesting, cell pellets were cross-linked with 0.75% PFA/PBS for 10 min, quenched in 1 M glycine, washed in PBS, and cell pellets snap frozen in liquid nitrogen. Cells were lysed in ChIP lysis buffer (50 mM HEPES-KOH pH 7.5, 140 mM NaCl, 1 mM EDTA pH 8, 1% Triton X-100, 0.1% sodium deoxycholate, 0.1% SDS, Protease Inhibitor Cocktail) and sonicated to shear chromatin to approximately 300bp DNA fragment size. Input and ChIP fragments were quantified using QUBIT dsDNA kit and 5 ng DNA fragments was subjected to library preparation using NEBNext Ultra II DNA library preparation kit for Illumina.