GSM6883014: ChIPseq_BJ_R227Q_exp1; Homo sapiens; ChIP-Seq
Cells were cross-linked with 1% formaldehyde in PBS, lysed in SDS lysis Buffer for 10 min in ice, and sonicated using a Bandelin Sonopuls device. The immunoprecipitated DNA was purified using MinElute PCR purification kit (#28004, Qiagen) library construction was performed using KAPA Hyper Prep Kit (Kapa Biosystems, #KK8504) The prepared libraries were then sequenced using the NextSeq 500 (Illumina) according to standard Illumina protocols