GSM6757894: GL261-Nes-high-1; Mus musculus; RNA-Seq
Total RNA was extracted using RNeasy Mini kit (Qiagen) RNA-seq libraries were constructed using 100ng to 1ug of RNA using Illumina library structure (Stranded mRNA Library preparation, Illumina), according to the manufacturer's instructions. For MGG4-BV samples only, SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian protocol was used. Samples were sequenced as 100bp paired-end runs on the Novaseq 6000 (Illumina).