GSM7832512: EE-AI [21_EndVivo-6]; Bos taurus; RNA-Seq
The total RNA of endometrial explants (EE, EE-AI, and EE-IVF) was extracted using miRNeasy Mini Kit (QIAGEN) following the manufacturer's instructions. The total RNA was treated with DNase I (Invitrogen; Carlsbad, CA) to avoid DNA contamination. The RNA library preparation of three replicates of endometrial explants co-cultured in the presence or absence of in vivoorin vitroproduced bovine embryos were performed with 0.1- 4 µg the total RNA using Illumina TruSeq Stranded mRNA Sample Prep kit. The libraries were quantified by qPCR using the KAPA Library Quantification kit (Illumina; KAPA Biosystems). The sequencing was performed in 1 lane of Hiseq 2500 V4 (2x100pb).