Illumina secuencing of C. longiscapa genetic diversity leaf
igh-quality DNA was prepared from frozen leaf material of C. longiscapa harvested from locations 2, 3 and 5. Cuticles were removed with a scalpel prior to grinding in liquid nitrogen, and the ground tissue was lyophilized until the sample was completely dry for 24 hours. A total of 1 mg of lyophilized tissue was processed with the MasterPure Complete DNA and RNA purification kit (Illumina) according to the protocol provided by the supplier. The DNA integrity was verified by gel electrophoresis, and total DNA was quantified by fluorometry with Qubit dsDNA HS assay (Invitrogen). Twelve libraries were constructed using the TruSeq DNA Nano kit