GSM6979392: HUVEC, siCTRL; Homo sapiens; RNA-Seq
For multiplexed scRNA-sequencing of cultured ECs, HUVECs were labeled with sample tag human antibodies using the BD Human Sample Multiplexing Kit (BD Bioscience, 633781). Briefly, cells were trypsinized, centrifuged and resuspended in 2% FBS/PBS and incubated with 10 µl sample tag antibody per 1.5x10^5 cells for 20min on ice. After several washes with 2% FBS/PBS, cells were loaded into a BD Rhapsody Cartridge (BD Bioscience, 633733) and captured on the BD Rhapsody Express Single-Cell Analysis System (BD Bioscience). Whole transcriptome mRNA and Sample Tag libraries were created using the BD Rhapsody Whole Transcriptome Analysis (WTA) Amplification Kit (BD Bioscience, 633801) and DNA sequencing was performed on a NextSeq500 (Illumina).