GSM6615322: PBMCs_CLP+ADSCs2_4; Mus musculus; RNA-Seq
Total RNA was extracted from PBMCs samples using TRIzol reagent (Thermo Fisher, USA) according to the manufacturers instructions. The concentration of RNA was determined by Gene Quant pro 1000 (Thermo Fisher, USA). Total RNA quality was assessed via RNA integrity number (RIN) using a Bioanalyzer 2100 (Agilent, USA). Then, cDNA libraries were constructed, and the final average insert size was 300±50 bp.