GSM7706788: Mouse 1 LT-HSCs CD61 Low; Mus musculus; RNA-Seq
RNA isolation:  RNA isolation using RNeasy Plus Micro Kit (Quiagen) cDNA conversion was done using SMART-Seq v4 Ultra low input RNA kit for sequencing (TAKARA Bio, 4ng total RNA was used). Library preparation: Kit used for library preparation: Nextera XT DNA Library Prep, preparation was performed according to the protocol. 1ng of amplified cDNA was used and the remaining volume was supplemented with EB buffer. A 0.6x bead cleanup was performed. Samples were eluted in 14μl EB. Tagmentation was used for primer attachment