Illumina HiSeq 1500 paired end sequencing; RNA-seq of human breasr epithelial cell line MCF10A treated for 8 days with the CDC7 inhibitors XL413, TAK-931 against DMSO-treated controls
RNA-seq of human breasr epithelial cell line MCF10A treated for 8 days with the CDC7 inhibitors XL413, TAK-931 against DMSO-treated controls
Cells were harvested, and pellets were stored at -80°C in RNAlater (Merck). Eight days of treatment with CDC7 inhibitor was performed as follows:MCF10A were plated on 60 mm plates. After four days, cells reached confluency and were replated in 60 mm plates at the same seeding density described above. Treatment with DMSO, XL413 or TAK-931 was administered immediately following the replating and continued to day 8. XL413 (synthesised in-house) was used at a concentration of 20 μM and TAK-931 (Chemietek) was used at a concentration of 0.625 μM for 8 days RNA was purified from MCF10A EditR cells using the QIAshredder and the RNeasy Plus Micro Kit (Qiagen), as per manufacturers' instructions. The purified RNA was stored at -80°C. RNA library is formed by polyA capture and reverse transcription of cDNA (Performed by Novogene)