GSM7043000: Y1 7.4 48hrs [707-505Y17.448]; Mus musculus; RNA-Seq
100 LT-HSCs were sorted and processed as recommended by Clontech SMART-Seq v4 Ultra Low Input RNA Kit. The kit generates Illumina compatible RNA-Seq Libraries The amplified cDNA generated were used to make libraries using Illumina's Nextera XT DNA Library Preparation kit (Catalog # FC-131-1096) as per Illumina's instruction. The generated libraries were quantified using agilent bio-analyzer, pooled and subjected to next generation sequencing in Hi-Seq 2500, for pair end 75bp sequencing condition.  Libraries generated are Illumina platform compatible.