Chromosome flow sorted shotgun sequencing of chromosome 1H from barley accession Baronesse, insert size of 221 bp
Chromosome flow sorting of Baronesse chromosome 1H was performed using the methods described by Dolezel et al. (2012) Functional and Integrative Genomics 12, 397-416 and chromosomal high molecular weight (HMW) DNA was prepared as described in Thind et al. (2017) Nature Biotechnology 35, 793-796. Sequencing library was generated using NEBNext Ultra enzymes (New England Biolabs) and Illumina-compatible adapters and sequenced using Illumina HighSeq X using insert size, 221 bp.