RNA-Seq of KS Skin Lesion
Approximately 30 mg tissue was processed using the Qiagen, RNeasy Fibrous Tissue Mini Kit (Qiagen, cat# 74704). Qubit assay (Life Technologies) and on a 4200 Tape Station (Agilent Technologies) were used for RNA quantity and quality verification. The Ion Chef System (Life Technologies Pub. No. MAN0016854 Rev. F.0) for sequencing on the Ion Torrent S5 sequencer (Life Technologies Pub. No. MAN00006735 Rev: F.0.) were used for Library preparation and sequencing respectively.