GSM6586003: mouse-muscle-cell-differentiating-4B-RNASeq; Mus musculus; RNA-Seq
After purification with the MinElute Reaction Cleanup Kit, 3 ng ds-cDNA was processed for library construction using KAPA Hyper Prep Kit according to the standard protocol except that a 15-minute USER enzyme incubation step was added before library amplification. The prepared libraries were quantified with the KAPA Library Quantification Kit, and then sequenced in a paired-ended manner using the NextSeq 500 (Illumina) according to standard Illumina protocols.