DNA from E. coli cells with pBADHisA_XauSPARDA from Xanthobacter autotrophicusPy2 and pCDFDuet plasmids
To obtain XauSPARDA fragmented DNA E. coli BL21-AI was transformed with pBADHisA_XauSPARDA and pCDFDuet plasmids. Cells were grown at 37 C in LB medium in the presence of 50g/ml ampicillin and 25 g/ml streptomycin until OD600=0.5 was reached. Then, expression was induced by adding 0.2% w/v L-arabinose, and cells were harvested after 2 h. DNA was purified using GeneJET Plasmid Miniprep Kit (Thermo Scientific cat#K0502). The DNA library was prepared using CROFT-seq method (US patent appl. no. 18/326,486) and sequenced using Illumina MiSeq sequencing with pair-end reads and 150 bp read length.