M.verrucosa (5DR)
DNA from the sample was end-repaired (NEBnext Ultra II end repair kit), cleaned up with 1x AmPure beads. Native barcode ligation was performed with NEB blunt/ TA ligase (using NBD104 (ONT) and cleaned with 1x AmPure beads. Adapter ligation of Qubit-quantified barcode ligated DNA sample (BAM) was performed for 15 minutes using NEBNext Quick Ligation Module. Library mix was cleaned up using 0.6X AmPure beads and finally sequencing library was eluted in 15 l of elution buffer and used for sequencing.