Low coverage whole genome sequencing of Colaptes auratus
DNA was extracted using DNeasy blood and tissue extraction kits (Qiagen, California, USA) following the manufacturer's protocol. Extracted DNA was quantified using a Qubit fluorometer (Life Technologies, California, USA). 200 ng of DNA was used to prepare individually barcoded libraries with a 500bp insert size with the TruSeq Nano DNA Library Prep kit (Illumina, California, USA). Libraries were pooled into three groups and sequenced on separate lanes of an Illumina NextSeq500 (2x150bp) at the Cornell University Biotechnology Resource Center.