CRISPR/Cas9 knockout screen of the HCC cell line HLF
Genomic DNA was extracted using the Zymo Quick-gDNA MidiPrep Kit (Zymo Research Europe, Freiburg, Germany). Library prep was performed as previously described57 using the NEBNext Ultra II Q5 Polymerase (New Engladnd Biolabs, Frankfurt, Germany) and purified with AMPure Beads (Beckmann Coulter, Krefeld, Germany). The Illumina NextSeq 550 (Illumina, San Diego, CA, USA) was used for sequencing of pooled samples