GSM8169899: LB; Homo sapiens; RNA-Seq
Fresh isolated human oral mucosal tissue specimens were rinsed with HBSS, cut into small pieces, and filtered twice through 40um filters for single-cell analysis. Guided by 10X manufacturer's protocols, single cell cDNA library was prepared and sequenced. Briefly, single cell suspension was loaded on a Chromium controller to obtain single cell GEMS (Gel Beads-In-Emulsions) for the reaction. The library was prepared with Chromium Next GEM single cell 3' kit V2 (10X Genomics) and sequenced on Illumina Novaseq 6000.