GSM8403781: TS, scRNA-seq; Homo sapiens; RNA-Seq
The day 6 ppTOs were removed from Matrigel using Cell Recovery Solution at 4°C for 30 min. Then, the ppTOs were further digested into single cells using TrypLE Express Enzyme. The enzymatic treatment was performed on a shaker at 37°C for 20 min, and pipetted every 5 min. The cells were collected by centrifugation and filtered through a 40 μm Cell Strainer. The cell viability was detected using trypan blue staining. The construction of transcriptome libraries was performed following the standard protocol of 10X Genomics (v3). Briefly, the cDNA was purified after GEM generation, barcode addition, GEM-RT reaction and bead cleanup. The GEX libraries were generated following the protocol of 10x Genomics Chromium Single Cell 3' Reagent Kits (v3). The concentration of each library was accurately detected for 10x Genomics sequencing.