GSM7524125: HPMEC cells treated with TNF replicate 2; Homo sapiens; RNA-Seq
Cell monolayers were washed with sterile phosphate buffered saline (PBS, Invitrogen) and total RNA was purified using the RNeasy Mini Kit (Qiagen) with an on-column DNase treatment. Poly-A, strand-specific RNA library construction was performed by the Yale Center for Genomic Analysis utilizing the TruSeq Library Prep Kit 2.0 per the manufacturers specifications. . Following first-strand synthesis with random primers, second strand synthesis was performed with dUTP for generating strand-specific sequencing libraries.