GSM8442228: CBL HET C404Y 89 NT 3; Homo sapiens; RNA-Seq
Phase separation in cells lysed in 1 mL TRIzol Reagent (ThermoFisher Scientific) was induced with 200 µL chloroform and RNA was extracted from the aqueous phase using the miRNeasy Mini Kit (Qiagen) on the QIAcube Connect (Qiagen) according to the manufacturer's protocol with 350 µL input, or using the MagMAX mirVana Total RNA Isolation Kit (ThermoFisher Scientific) on the KingFisher Flex Magnetic Particle Processor (ThermoFisher Scientific) according to the manufacturer's protocol with 350 µL input. Samples were eluted in 30 µL RNase-free water PolyA selection and TruSeq library preparation according to instructions provided by Illumina (TruSeq Stranded mRNA LT Kit, Illumina), with 8 cycles of PCR.