GSM6503481: ATTX-INTACT.112-RNA; Homo sapiens; ssRNA-seq
Total cellular RNA was extracted using the Norgen RNA Purification PLUS kit (#48400, Norgen Biotek Corp) as per manufacturer's instructions. RNA quality (RIN>8) and quantity were determined on an Agilent 2100 Bioanalyzer (Agilent Technologies) and Qubit®. 2.0 Fluorometer (Thermo Fisher Scientific). RNA libraries for RNA-seq were prepared using the Illumina TruSeq Stranded mRNA Sample Prep Kit (strand-specific, polyA enrichment), including ERCC controls. Paired-end sequencing on Illumina HiSeq 2500 (Kinghorn Centre for Clinical Genomics, Sydney, Australia) with a read length of 125 bp, yielding about 40 M read pairs per sample.