GSM6615080: WT; Mus musculus; RNA-Seq
WT and mutant nimals were sacrificed and perfused in cold PBS. The vHPC was dissected from the fresh brain, and digested in Papain supplemented with DNAse I. After dissociation and filtering, cells were concentrated to 1,000,000 cells/ml and loaded on a v3 chip in the 10x Genomics Chromium Controller. Libraries were prepared using the the 10x Genomics NEXT-GEM Single Cell 3' Reagent Kits according to the manufacturer's protocol. 3' mRNA-seq