GSM7653812: ANP32E-KO-DMSO_RNA-Seq biol rep 2; Mus musculus; RNA-Seq
gentle trypsin extraction RNA-Seq libraries were prepared using the TruSeq Stranded Total RNA Library Prep Kit (cat: 20020598), according to the standard methods, and total RNA was purified using the Zymo Research Direct-zol RNA Miniprep kit (cat: R2051). Libraries were sequenced in 150 bp paired-end sequencing mode on an Illumina NextSeq550 high-throughput DNA Sequencer. CUT&Taglibraries were generated using approximately 100,000 cells. Cells were incubated with Convanavalin A-coated magnetic beads (Bang Laboratories, BP531), and then primary antibodies were added to incubate overnight at 4 °C. After incubation, secondary antibodies (incubated at RT for 1 h) and pA-Tn5 adapter complex (incubated at RT for 1 h) were added. Then, tagmentation took place for 1 h, and DNAs were extracted using phenol–chloroform–isoamyl alcohol. Libraries were prepared using NEBNext HiFi 2XPCR Master mix and cleaned up using Ampure XP beads (Beckman Coulter A63880). CUT&Tag and RNA-Seq