GSM8338518: ApoE,high fat diet, rep8; Mus musculus; RNA-Seq
The tissues were frozen in liquid nitrogen, transferred into Trizol, and then homogenized using a manual Omni tissue homogenizer (TH2, 2308) and dedicated hard tips. All the following steps of RNA isolation were carried out according to Total RNA Zol-Out (A&A Biotechnology) protocol for the rapid purification of ultra-pure total RNA from samples prepared in TRIzol (A&A Biotechnology) RNA-seq libraries were prepared from 1ug of total RNA using NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (NEB) together with NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) and NEBNext® Multiplex Oligos for Illumina® (NEB) according to manufacturer's protocol