GSM8075339: coD14_WT_CUTTAG_H3K27me3_rep1; Homo sapiens; OTHER
Mouse brain tissues and human cortical organoids were digested with Papain to single cell, then about 5 x 104 cells and 0.5-1 μg antibodies or IgG were used for the downstream procedure with 12 cylcles during the final amplification PCR. Cut&Tag-Seq was performed following manufacturer's instructions (Vazyme, TD904).