MiSeq_coel_W_16
5L seawater was filtered through a 0.22 m Sterivex filter (Millipore) using a vacuum pump. DNA extractions was done in a sterile laboratory using the E.Z.N.A  Tissue DNA Kit (Omega Bio-tek) following manufacturers' protocol. PCR was done in triplicate in 25 L reactions and sequenced on an Illumina MiSeq.