GSM6585974: NTC-rep5; Mus musculus; RNA-Seq
HSCs were sorted into lysis buffer. RNA-Seq was performed according to Smart-seq2. The RNA was reverse transcribed by SuperScript II reverse transcriptase (Invitrogen) and amplified to enrich the cDNA fragments. The cDNA was purified by VAHTS DNA Clean Beads (Vazyme). Tn5 transposase was used to tagment DNA, then stripped by 0.2% SDS. The DNA was amplified by Q5 High-Fidelity 2× Master Mix (NEB) followed by VAHTS DNA Clean Beads purification.