Gill transcriptome_control_R2
2_The paired-end sequencing library was prepared using NEB NextUltra RNA Library Prep Kit for Illumina (NEB #E7770) The library preparation process was initiated with 1000 ng input. The mRNA enrichment was performed as per the user manual and mRNA was subjected to fragmentation, first & second-strand cDNA synthesis, end-repair, 3 adenylation, adapter ligation, and selective enrichment of adapter-ligated DNA fragments through PCR amplification. The validation of the library was performed on Agilent 4150 tape station using HSD1000 ScreenTape as per the manufacturer's instructions. The final library was denatured & loaded onto the flow cell. On the flow cell, cluster generation & sequencing were performed using the Illumina Novaseq 6000 platform to generate 2150bp paired-end (PE) reads and ~8-10GB data/Sample.