GSM8037229: Direct RNA seuqence of DMSO treated HEK293T cell replicate 2; Homo sapiens; RNA-Seq
Total RNA was extracted from the HEK293T cell line using TRIzol reagent (Invitrogen, #15596018), according to the manufacturer's instructions. RNA concentrations and RNA integrity were measured using a NanoDrop 2000 Spectrophotometer and Agilent 4200 TapeStation, respectively. A 20-μg aliquot of total RNA was subjected to poly(A)+ RNA selection using the Dynabeads mRNA DIRECT purification kit (Invitrogen, #61011) following the manufacturer's instructions. Subsequently, approximately 500 ng of poly(A)+ RNA was used for direct RNA library preparation. The libraries were generated following the standard ONT SQK-RNA002 protocol with the reverse transcription step included. The libraries were loaded onto R9.4.1 flow cells (ONT, FLO-PRO002) and sequenced on PromethION 2 Solo (ONT, PRO-SEQ002).