GSM6615276: KO452; Mus musculus; RNA-Seq
Was -/- (129S6/SvEvTac-Wastm1Sbs/J) (WAS-Knockout, WAS-KO) and 129S6/SvEvTac (wild-type, WT) mice were purchased from the Jackson Laboratory (Bar Harbor, Maine, ME, USA). All animals were maintained with food and water made available ad libitum and under a 12 h light/dark cycle. Ten-week-old male mice were used in this experiment, and all experiments were approved by the Medical Ethics Committee of Children's Hospital of Chongqing Medical University. The mice were euthanized, and their spleens were isolated. Total RNA was extracted from the right spleen using an RNAqueousTM Kit (AM1912, Thermo Fisher, Germany) following the manufacturer's instructions. Purity was determined by a NanoDrop 1000 (Thermo Scientific, Germany). RNA libraries for RNA-seq were prepared using  illumina mRNA-Seq Library  Kit following manufacturer's protocols.