mRNAseq of QX2345 NIL young adults repeat 1
Worms were washed multiple times using M9, followed by incubation with TRizol and chloroform. Phase-separating columns were used to separate the TRIzol and the aqueous phase was mixed with an equal volume of isopropanol. Following centrifugation, the pellet was washed multiple times and resuspended in RNase-free water. Only RNA samples with RIN>8 were used for library preparation using the NEBNext Poly(A) kit.