GSM8374132: cr4x_x_cg-endosperm.sRNA.WT.replicate1; Capsella rubella x Capsella grandiflora; ncRNA-Seq
For each cross around 600 seeds at 6 DAP were opoened and the endopserm was manually dissected. sRNAs were extracted using the mirVana miRNA isolation kit (Ambion/Life Technologies) following the enrichment procedure for small RNAs resulting in a small RNA fraction ≤200nt. Libraries were prepared with NEBNext® Multiplex Small RNA Library Prep Set for Illumina® using 50 -100 ng of input for each sample. Size selection was performed with 6% polyacrylamide gel; bands corresponding to about 150 bp were cut and purified from the gel for further analysis. Sequencing was performed at Novogene (Hongkong, China) on a NovaSeq 6000 platform in 150-bp paired-end mode.