Profiling of hot spring microbiomes using V4-region of 16S rRNA gene
DNA was isolated from wet sediments using FastDNA Spin Kit for Soil (MP Biomedicals, USA) including mechanical cell lysis using a FastPrep-24 5G homogenizer (MP Biomedicals, USA). Amplicon libraries were prepared using primers for the V4 region of 16S rRNA gene. PCR was performed on a StepOne Plus Real-Time instrument (Thermo Fisher Scientific, USA).