GSM7967313: ES489, sam2∆/sam2∆ replicate 3; Saccharomyces cerevisiae; RNA-Seq
RNA extraction follows the RNA Isolation procedure from the TRIzol Reagent protocol (Ambion Cat #15596-018). RNA Purification was completed using the RNeasy RNA Cleanup protocol and spin columns, estimating for up to 1mg of RNA (Midi kit), from Qiagen, with DNase treatment on the columns and removal by subsequent washes. RNA quantity and quality were assessed via Nanospec and gel analysis. Libraries were prepared using the TruSeq Stranded mRNA LT Sample Prep Kit- Set A (Cat# RS-122-2101) and Set B (Cat# RS-122-2102) with poly-A enrichment.