GSM8403780: H69AR cells, siTREX1, rep 3; Homo sapiens; RNA-Seq
Total RNAs were extracted and purified using the Rneasy MiniKit (QIAGEN) from the cells at day 3 post-transfection. Using a 2100 Bioanalyzer RNA 6000 Nano assay (Agilent), quality of RNA was assessed. RNA concentration was measured using a Qubit 2.0 Fluorometer (Life Technologies). Illumina sequencing libraries were constructed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB).