GSM6758137: sciATACv2 nanowell-based preparation on mouse whole brain tissue; Mus musculus; ATAC-seq
Nuclei were extracted by mincing tissue with a razor blade in ice cold using Nuclei Isolation Buffer (NIB) followed by dounce homogenization in NIB, then spinning down at 500xg, removing the supernatant and resuspending in NIB. Single-cell combinatorial indexed ATAC-seq was performed by tagmenting nuclei in 96-well plates using uniquely barcoded tagmentaiton complexes followed by pooling tagmented nuclei from all wells, washing in NIB, then distributing nuclei to a 5,184 nanowell chip using the iCell8 instrument for indexed PCR amplificaiton, pooling, and sequencing.