GSM8338554: TTFs, pMXs-PHF7, Day 7 iCLM reprogramming, replicate 1; Mus musculus; RNA-Seq
Total RNA was extracted from TTFs seven days after retroviral transduction, using TRIzol (Invitrogen) according to the vender's protocol. Illumina RNA-seq was performed by the University of Texas Southwestern Microarray Core Facility. Library construction was performed per manufacturer protocol for mRNA library construction. Illumina RNA-seq was performed by the University of Texas Southwestern Next Generation Sequencing Core Facility.