Full-length transcriptome sequencing of Ganxi goat skin
Twelve 1-year-old clinically healthy female Ganxi black goats with a body weight of 18.10.68 kg were collected from the same group. The experiment was carried out after 1 week in the same environment with single cage feeding and free feeding. They were randomly divided into 4 groups (n=3), 3, 5, 7 and 9 d groups. After anesthesia and disinfection, a full-layer incision of 5 cm long skin was made in the middle and upper part of the left ventral side. 2 cm2 skin tissue (as the sample on the 0d after trauma) was immediately taken and divided into two equal parts. One part was placed in 4% paraformaldehyde solution prepared with 0.1 M PBS pre-cooled at 4  for fixed use. The other was transferred to -80  after using RNA Keeper Tissue Stabilizer at 4  for 24 h. The wound was sutured with nodules and protected with gauze. Subsequently, the skin tissue of the wound was collected by the same method on the 3rd, 5th, 7th and 9th day after the trauma. According to the experimental results, 3 cases of skin Wound tissues were selected on day 0 (Control group, Control Check, CK) and day 5 (test group, Wound Team, W) after skin surgery. A total of 6 total RNA samples of Ganxi goats were mixed in equal proportions as sequencing samples (Ch). Full-length transcriptome sequencing was performed by Nanjing Peisenor Gene Technology Co., LTD.