GSM8214610: GC119663_2010_Mutant; Homo sapiens; RNA-Seq
Total RNA was extracted using the Quick-RNA Microprep Kit Libraries were prepared from 500 ng using the QuantSeq 3' mRNA-seq library prep kit (Lexogen) Libraries were pooled equimolar to 2 nM for single-read sequencing on the HiSeq4000 (Illumina) with settings 51-8-8