Indo-Pacific king mackerel muscle Hi-C
Following the standard protocol described previously with certain modifications, Hi-C libraries were constructed using the genome DNA as inputs, the DNA was digested using MboI, and Biotin-labeled Hi-C samples were specifically enriched using streptavidin C1 magnetic beads. After adding A-tails to the fragment ends and following ligation by the Illumina paired-end (PE) sequencing adapters, Hi-C sequencing libraries were amplified by PCR (12-14 cycles) and sequenced on Illumina NovaSeq-6000 platform (PE 150 bp).