GSM6926613: non-lesional ear skin from B6 control mice [B6-3_S11_L002]; Mus musculus; RNA-Seq
Female MRL/+ and MRL/lpr 12-14 weeks old and 6 month old B6 and B6.Sle1yaa male mice were used for RNA sequencing. RNA was extracted from ear skin using an RNEasy Kit (Qiagen, Germantown, MD) and quality confirmed on a BioAnalyzer 2100 (Agilent Technologies, Santa Clara, CA) Libraries were prepared with non-stranded and poly-A selection TruSeq RNA Sample Preparation kits. Sequencing libraries were sequenced by the Genomics Core Facility at Weill Cornell Medicine using a HiSeq 2500 (MRL/lpr, MRL/+ samples) and NovaSeq (B6, B6.Sle1yaa) at a depth of 15-30 million mappable paired-end reads per sample.