GSM8263009: T24+IAA replicate 1; Homo sapiens; RNA-Seq
Total RNA was extracted using NuclepSpin RNA plus (Takara) following the protocol. For mRNA isolation, NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II RNA Library Prep Kit for Illumina were used. While analysis was conducted using the NextSeq 500/550 High Output Kit v2 (75 Cycles).