RSV F gene sequence: nasal swabs
RT-PCR amplification of an RSV target sequence from nucleic acids that have been extracted from nasal swab specimens. The amplification step is followed by purification of the PCR products, visualization and concentration determination. Amplified samples are diluted and libraries are prepared and indexed using the Illumina Nextera XT Library Prep Kit.  Following a bead-based purification step, prepared libraries are quality checked.  Libraries are then diluted, pooled, denatured, and loaded into a sequencing reagent cartridge for processing on the Illumina MiSeq instrument.