scRNA-Seq of mus musculus: adult male retina
The single-cell suspension was obtained from the retina and choroid tissues by digestion of digestive enzymes at 37 water bath for 30 min-4 h. The cell is counted after removing the enzyme. After the detection rate of live cells reached more than 80%, single-cell transcriptome libraries were constructed by the Chromium Single Cell 3 v3.1 Reagent Kit (120237; 10X Genomics) and high-throughput sequencing were performed.