GSM8374421: MB453_CRISPR lib_DOX_RGS-Res4; Homo sapiens; RNA-Seq
RNA from control cells or the rigosertib-resistant colonies was extracted with the mirVana miRNA extraction kit (AM1571, Invitrogen) following the manual instructions Total RNA samples (250 ng) with an average RIN=9.6 (range: 9.4-9.8) in a labchip analysis, were processed into cDNA sequencing libraries with the "QuantSeq 3' mRNA-Seq Library Prep Kit (FWD) for Illumina" (Lexogen, Cat.No. 015). Briefly, library generation is initiated by reverse transcription with oligodT priming, followed by a random-primed second strand synthesis. Primers from both steps contain Illumina-compatible sequences. Libraries were completed by PCR, and sequenced on an Illumina NextSeq 550 (with v2.5 reagent kits) instrument by following manufacturer's protocols.