CK_2:miRNAs-seq of tea leaves punctured by sterilized syringe needles, followed by tissue samples being harvested at 2 days post-inoculation.
Samples from 15 plants at each treatment group were pooled together to avoid variations in individual plants due to pathogen inoculation. Leaf samples were ground into fine powder under the protection of liquid nitrogen. About 100 mg powder of each tea leaf was used for RNA extraction. rRNA depletion was used to construct the chain specific library The libraries were sequenced on an Illumina NovaSeq 2500 (LC Bio, China) following the vendor's recommended protocol.