GSM8169895: ScRNA, Plerixafor; Mus musculus; RNA-Seq
Single-cell 5′ and V(D)J libraries of the T-cell receptor (TCR) were prepared in accordance with the protocol provided by the 10x Genomics Chromium Single Cell Gene Expression platform. Cell suspensions containing 700-1,200 viable cells per microliter (with a viability of at least 85%, as determined by CounterStar) were loaded onto a Chromium Single Cell Controller (10× Genomics). This step facilitated the generation of single-cell gel beads in emulsion (GEMs) by employing Chromium Single Cell V(D)J Reagent Kits. After the cells were captured, they underwent lysis, and the resulting RNAs were barcoded via reverse transcription, all within individual GEMs. Subsequently, each single-cell 5′ and V(D)J library was subjected to sequencing using the Illumina Novaseq 6000, employing 150 paired-end reads for comprehensive analysis