GSM8441013: Cont3; Mus musculus; RNA-Seq
Total RNA from neutrophils were isolated using RNeasy plus mini kit (Qiagen). Total RNA was preamplified with SMARTer Ultra Low Input kit v4 per manufacturer's protocol (Clontech). CDNA quality and quantity were measured using Qubit fluorometer (Life Technologies) and Agilent Bioanalyzer 2100. CDNA was used to generate Illumina-compatible sequencing libraries using NexteraXT library preparation kit (Illumina). Clontech low input/Illumina mRNA