mRNA-seq 48hrs AZD8055__replicate 3 plate: H2FVMDRX2
Total RNA was isolated from cells with QIAzol and thethe miRNeasy kit (QIAGEN, 217004) with on-column DNAse digestion (QIAGEN, 79256) per manufacturers instructions. mRNA was isolated with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7760) following instructions of the manufacturer. R2 file = UMI.