GSM8431789: Loaded, MLO-Y4, H3K27ac, rep2, ChIP; Mus musculus; ChIP-Seq
MLO-Y4 cells of each group were fixed by 1% formaldehyde solution (Sigma, F8775-25ML) and quenched with 1.25M glycine (Amresco, 200-272-2). Chromatin was collected and sheared using the Bioruptor Plus with 30 seconds on and 30 seconds off for 18 cycles. Immunoprecipitation was performed using 3–5 μg of H3K27ac (Abcam, ab4729); H3K4me3 (Abcam, ab8580) or NRF2 (Proteintech, 80593-1-RR) antibodies. DNA was purified using the DNA Clean & Concentrator-5 kit (Zymo, D4014). Libraries were then constructed from ChIP samples using the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, E7370) and sequenced on a NovaSeq by Annoroad Gene Tech (Beijing) Co., Ltd. Two biological replicates were performed for each group.