GSM8075118: EO771 tumor, Pcsk9 WT mouse, biol rep3; Mus musculus; RNA-Seq
Total RNA from primary tumors from mice was isolated with the Total RNA Purification Kit (Norgen Biotek, 17200) with 5ul/ml of RNasin Plus (Promega, N2615) and 10ul/ml of 2-mercaptoethanol (Sigma-Aldrich, M6250) added to buffer RL. Libraries were constructed from 1μg of total RNAs using the TruSeq RNA Library Prep Kit (Illumina, RS-122-2001)