GSM7648673: Gar15-13 Vehicle 2; Homo sapiens; Bisulfite-Seq
Samples snap-frozen in Optimal Cutting Temperature compound (Tissue-Tek) were used for WGBS experiments. Using a cryostat (Leica, #CM3050-S), 15x 30 µm sections were cut from each tumour at  -20 ̊C and these samples were subjected to DNA extraction. Library preparation and indexing were carried out as described in the CEGX TruMethyl WG user guide v2 wih minor improvements as described in Nair et al., 2018.