GSM8214783: WI-38, replicative senescence, RNA-seq, Rep2; Homo sapiens; RNA-Seq
RNA was extracted using Dynabeads® mRNA DIRECT (Thermo Fisher) to deplete ribosomal RNA and avoid DNA contamination. The purified product was divided into two parts, one for RNA-seq and the other for MeRIP-seq. The fragmented mRNA underwent m6A IP using the EpiMark® N6-Methyladenosine Enrichment Kit (NEB) following the manufacturer's protocol. RNA libraries were prepared for both input RNA and m6A-enriched mRNA after IP using the VAHTS Universal V8 RNA-seq Library Prep Kit for Illumina (Vazyme) according to the manufacturer's instructions.