GSM7867856: WT biol rep 1; Danio rerio; RNA-Seq
After genotyping, for each WT and bcas2-KO sample, 10 zebrafish embryos were used to isolate total RNA using the TRIzol reagent (Invitrogen, USA) according to the manufacturer's protocol. Sequencing libraries were generated using the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer's technical guide. The library preparations were then sequenced on an Illumina Hiseq platform using the 2 x 150 bp paired-end configuration according to the manufacturer's protocol. For each cDNA library, 6 G base paired-end raw reads were generated.