GSM8432466: HFSC, WT, 1; Mus musculus; RNA-Seq
Viable CD45-CD34+Sca-I- cells were sorted from epidermal single cell suspensions of 1-month-old EGFRΔEgr2 (n=3) and littermate controls (n=3) into Trizol LS Reagent (Thermo Fisher Scientific) using a FACS Aria III. RNA was extracted with the column-based miRNeasy Micro Kit (Qiagen) according to the manufacturer's protocol. cDNA synthesis was performed with Superscript IV Reverse Transcriptase (Thermo Fisher Scientific). Samples were sent to Novogene, UK for quality control, library preparation and sequencing using Novaseq 6000 PE150.