shotgun metagenomics of infant stool and maternal breastmilk
DNA sequencing was performed at the George Washington University Genomics Core. DNA extraction was performed for stool and breast milk samples using the Zymo Quick-DNA Fecal/Soil Microbe DNA Miniprep kit. Libraries were prepared using the Nextera XT kit and sequenced using the NextSeq 500 High-Output 300 cycle kits (Illumina).