GSM8214901: monosome_WT_rep3; Oryza sativa Japonica Group; RNA-Seq
Monosomal or polysomal RNA was prepared from leaf using WizPrepTM Plant RNA Mini Kit (WIZBIOsolutions, Seongnam, South Korea) Extracted RNA was extracted from the leaves, and cDNA was synthesized using PrimeScript™ RT reagent Kit (TaKara, Japan). The resultant cDNA fragments were purifed, end-reparided, and ligated to index adapters following the Illumina protocol using Ribo-zero kit.