GSM6757988: mES cells, WT1, d3 [RNA-Seq]; Mus musculus; RNA-Seq
Total RNAs from cell lines were isolated using TRI Reagent according to manufacturer's instructions. Contaminant genomic DNA was eliminated with TURBO DNA-free Kit . RNA quality was verified using the Bioanalyzer RNA 6000 Nano kit. The libraries were prepared following the TruSeq Stranded Total RNA protocol from Illumina, starting from 1 µg of high-quality total RNA (RIN > 7)