DNA-Seq of wastewater
Sequencing was carried out on the basis of the hypervariable region V3-V4 of the 16S rRNA gene. Specific primer sequences 341F and 785R (16S analysis) were used to amplify the selected region and prepare the library. PCR was performed using Q5 Hot Start High-Fidelity 2X Master Mix. Sequencing was carried out on a MiSeq apparatus, in paired-end (PE) technology, 2x300nt, using the Illumina v3 set.