GSM8374280: SS CD8+CCR7+CD45RO- Naive cells; Homo sapiens; RNA-Seq
Total RNA was extracted from indicated T cell populations. RNA Tape Station (Agilent, USA) analysis was performed as per the manufacturer's instructions to assess the quantity and quality of RNA present in the sample The library was then amplified with 3' PCR primers containing sample indices and the Illumina clustering guides. Indexed libraries were pooled and sequenced on a NextSeq500 (Illumina)