16S amplicon sequencing of anaerobic digestion effluent
Sequencing libraries were generated using NEBNext Ultra DNA Library Prep Kit forIllumina (New England Biolabs, USA) following manufacturer's recommendations and indexcodes were added. The library quality was assessed on the Qubit@ 2.0 Fluorometer (ThermoScientific) and Agilent Bioanalyzer 2100 system. At last, the library was sequenced on anIlluminaHiseq2500 platform and 250 bp paired-end reads were generated.