GSM8403888: Papain ILC2-1; Mus musculus; RNA-Seq
Total RNA from sortedlung ILC2s mRNA profiles of PBS treated mice and Papain treated mice was extracted by Trizol reagent.  RNA quality was examined with an Agilent Bioanalyzer 2100 (Agilent). The concentration of RNA was from 7ng/ul to 14ng/ul. The SMART-Seq HT Kit (Takara Bio USA, Inc) was used to generate high-quality, full-length cDNA. Libraries were generated using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme) following manufacturer's protocol.