GSM8432271: Python Regius, Heart, 24 hours post feeding replicate 3; Python regius; RNA-Seq
RNA was extracted using Tri Reagent (Molecular Research Center) and chloroform phase separation. 1 ug of total RNA was used for sequencing library preparation Libraries were generated using KAPA HyperPrep mRNA kit (Kapa Biosystems) per the manufacturer's instructions. RNA-seq libraries were prepared and sequenced by the BioFrontiers next generation sequencing facility using a Nextseq V2 highoutput 75 cycle kit. Libraries were sequenced single end 1x76bp with dual 8bp indexes.