Chicken CSF1R-eGFP cells_single-cell_RNA_sequencing, MAG_3, spleen
Splenocytes from 20 weeks-old female CSF1R-eGFP transgenic birds (n=2) were isolated and prepared for sorting. CSF1R-eGFP+ cells were sorted out from splenocytes using BD FACS Aria IIIu with the target of 5000 cells per sample. Libraries were prepared using Chromium Single Cell 3' Library & Gel Bead Kit v3 (Catalogue number #1000092) using 10X Chromium Single Cell RNA Sequencing Platform at the Henderson Lab at the University of Edinburgh. Libraries were pooled in equimolar quantities. Pooled libraries were sequenced by using an Illumina NovaSeq SP cycle setup to yield approximately 375M read pairs per lane at Edinburgh Genomics, University of Edinburgh.