GSM8019382: Overexpression-control-rep2; Mus musculus; RNA-Seq
NPCs were collected using cell scratchers and RNA was isolated using Rneasy Mini Kit (QIAGEN) with on-column DNase digest. RNA quality and integrity were controlled using Agilent Technologies 2100 Bioanalyzer. Libraries were prepared at Novogene using 1 µg of RNA and the NEBNext® Ultra RNA Library Prep Kit for Illumina® (cat# E7420S, New England Biolabs, Ipswich, MA, USA) according to the manufacturer's protocol. Libraries were quantified and  controlled using Agilent 2100 Bioanalyzer and sequenced on an Illumina Novaseq Platform (Illumina, San Diego, CA, USA) using a paired-end 150 run (2×150 bases).