GSM6879755: mm_chip_spreb_CTCF-BRWD1KO.2; Mus musculus; ChIP-Seq
Chromatin from flow-sorted small pre-B cells (2x10^6) from WT and Brwd1-/- mice, and WT small pre-cells treated with Oligomycin A, were used for each ChIP experiment with  anti-H3K4me1 (07-436, Millipore-Sigma), anti-H3K27Ac (07-360, Millipore-Sigma), anti-CTCF (Millipore Sigma, 07-729) , anti-RAD21 (Abcam, ab154769), anti-SMC3 (Abcam, ab9263), anti-NIPBL (Proteintech, 18792-1-AP) and anti-WAPL (Proteintech, 16370-1-AP) antibodies. Libraries were prepered by University of Chicago Core facility using Immumina Library preparation kit and sequenced on the Illumina NovaSeq 6000.