GSM6585800: Blastocyst rep2_add; Mus musculus; RNA-Seq
Total RNA was extracted with TRIzol reagent according to the manufacturer's instructions. TruSeq Stranded Total RNA kit(illumine) was used to construct library of early embryonic development stage following the manufacturer's instructions, each sample need approximately 150-200 embryos. In briefly, Ribosomal RNA (rRNA) was removed using biotinylated, target-specific oligos combined with beads, and cleaved RNA fragments were copied into cDNA using reverse transcriptase and random primers. cDNA subjected to 12–15 cycles of PCR amplification. For small amount total RNA-Seq, we used a SMARTer Stranded Total RNA-Seq Kit version 2(Takara) according to the manufacturer's instructions. The libraries were sequenced on an Illumina NovaSeq 6000 platform with paired ends and 150-bp read lengths at Berry Genomics.