YY_LC
Single-cell suspensions (1105 cells/ml) with PBS (HyClone) were loaded into microfluidic devices using the Singleron Matrix Single Cell Processing System (Singleron). Subsequently, the scRNA-seq libraries were constructed according to the protocol of the GEXSCOPE Single Cell RNA Library Kits (Singleron ). Individual libraries were diluted to 4 nM and pooled for sequencing. At last, pools were sequenced on Illumina NovaSeq 6000 with 150 bp paired end reads.