KGN-IRE1a-treated-replicate-3
Total RNA was treated with T4 Polynucleotide Kinase and separated on a 12% polyacrylamide gel containing 8 M urea to excise the 18-40nt region. RNAs were eluted from the acrylamide bands overnight in 0.3 M NaCl, then ethanol/glycogen-precipitated. Small RNA libraries were constructed using a SMARTer(R) smRNA-Seq Kit for Illumina(R) (Takara Bio) according to the manufacturers guidelines.