oak-associated ITS and 16S metagenome from leaves at 100% insect abundance, replicate 6
leaves were ground in liquid nitrogen and extracted with DNeasy Plant Mini Kit (Qiagen). Target sequences were amplified in triplicates with ITS4/ITS7 primers for fungi and 515f/806r primers for bacteria. Amplicons were cleaned with AMPure XP magnetic beads before and after adding Nexera XT indices. The purified PCR products were quantified with PicoGreen dsDNA Assay and combined to an equimolar pool. Pools were quantified and quality checked and sequenced with Illumina MiSeq.