GSM7867824: TFP_JAG8810_NS_biol rep3; Solanum lycopersicum; RNA-Seq
5-10 floral buds for each stage were sampled before and 20 days after the start of the heat stress from 20-25 plants per genotype for the RNAseq analysis (Figure S1B). Total RNAs were isolated from 200 and 500 mg of floral buds at different developmental stages using TRIzol Reagent (Life Technologies). Total RNA extract was purified with the Qiagen RNAeasy mini kit RNA. After DNase treatment (DNA-free Kit, Life Technologies), the total RNA quantity and quality (RNA integrity number, RIN) were evaluated using an Agilent 2100 Bioanalyzer (Agilent Technologies). Only RNA extracts with a RIN of 10 were used for sequencing. The RNA libraries were constructed as described in the Illumina TruSeq Stranded mRNA guide. mRNAs were sequenced in a HiSeq 3000 sequencing system with 2 × 125 bp paired-end sequences (Illumina HiSeq SBS Kit v4) by the Genotoul bioinformatics platform, Toulouse (http://bioinfo.genotoul.fr/index.php).