WGS of PC9
DNA was purified using QIAGEN DNeasy kit. Library preparation and sequencing was performed by Novogene. Briefly, 1 ug of genomic DNA was randomly sheared, and fragments were end-repaired, A-tailed, and ligated with Illumina adapters. Size selection was performed, followed by PCR amplification and purification. The resulting library was quantified with Qubit and RT-PCR, and bioanalyzer was used to determine the size distribution of the library. Libraries were then pooled and loaded onto an S4 flowcell and sequenced with NovaSeq 6000 with 10x genomic coverage per sample.