Short-read WGS sequencing of monoclonal PC3DM
Libraries were prepared by DNA tagmentation. We first transposed it with Tn5 transposase produced as previously described, in a 50-l reaction with TD buffer, 10ng DNA and 1 l transposase. The reaction was performed at 50C for 5 minutes, and transposed DNA was purified using Zymo DNA Clean & Concentrate kit (Zymo, 1159U33). Libraries were generated by 7 rounds of PCR amplification using NEBNext High-Fidelity 2 PCR Master Mix (NEB, M0541L), purified using SPRIselect reagent kit (Beckman 635 Coulter, B23317) with double size selection (0.8 right, 1.2 left) and sequenced on the Illumina Nextseq 550 platform.