RNAseq of Polycarpa mytiligera from FACS-purified cells
Cells from dissociated anterior tissues were purified by FACS directly into the RNA extraction media (TRIzol LS). About 10,000 cells were sorted per library. Following FACS purification, RNA was extracted from the TRIzol and integrity was assessed by microcapillary electrophoresis (TapeStation). Libraries were prepared using the NEB NEBNext Ultra II Directional RNA Library Prep Kit for Illumina.