GSM8336870: p720sPT-06TUMORCTRL2-30_xx_rna_sr_Conrady_R_1; Homo sapiens; RNA-Seq
RNA was isolated using the RNeasy Mini Kit (Qiagen) and processed as per manufacturer's instructions. RNA-seq libraries were performed using a modified non strand-specific, massively-parallel cDNA sequencing (RNA-Seq) protocol from Illumina starting with 200 ng RNA. The TruSeq RNA Library Preparation Kit v2, Set A (48 samples, 12 indexes; Illumina) was used.The size of final cDNA libraries was determined by using the dsDNA 905 Reagent Kit (Agilent) exhibiting a sizing of 260 bp in average. Libraries were pooled and sequenced on an Illumina HiSeq 4000 (Illumina) 13 generating 50 bp single-end reads (30-40 Mio reads/sample).