16S amplicon sequencing of wheat root/soil
All 16S rRNA amplicon sequencing amplified the V4 region using the 515F and 806R primers. ITS amplicon sequencing used the ITS1f and ITS2 primers. PCR products for the 16S rRNA and ITS amplicons were pooled separately and purified on a 2% agarose gel using a Qiagen gel extraction kit. The excised regions were ~385 bp for the 16S rRNA amplicons and 200 to 600 bp for the ITS amplicons.