GSM8374099: Microglia, invitro, mock, rep1; Mus musculus; RNA-Seq
RNA from “input” and RiboTag-IP fractions was extracted using Qiagen RNeasy Mini Kit following manufactures instruction. For cultured microglia, RNA was isolated using Direct-zol RNA Miniprep Plus Kit. RNA quality and concentrations were accessed using Bioanalyzer RNA kit and samples with RIN value >8.0 were selected for further processing. RNA libraries for RNA-seq were prepared using NEBNext Low input RNA library Prep Kit for Illumina (NEB, E6420) following manufacturer's protocols.