GSM7042967: ABP49; Mus musculus; RNA-Seq
RNA was extracted using Rneasy Mini kit (QIAGEN) Total RNA samples (250ng) were processed with the QuantSeq 3' mRNA-Seq Library Prep Kit (FWD) for Illumina (Lexogen, Cat.No. 015) by following manufacturer instructions. RNA quality scores were 7.2 on average (range 5.9-7.8) when assayed on a PerkinElmer LabChip analyzer. Library generation is initiated by reverse transcription with oligodT priming, and a second strand synthesis is performed from random primers. Primers from both steps contain Illumina-compatible sequences. Libraries are completed by PCR: this kit generates directional libraries stranded in the sense orientation, the read1 (the only read in single read format) has the sense orientation.  cDNA libraries are purified, applied to an Illumina flow cell for cluster generation and sequenced on an Illumina NextSeq 550 (with v2.5 reagent kits) by following manufacturer's protocols.