GSM7745556: KO_1; Mus musculus; RNA-Seq
Tumors were frozen in -80 until RNA isolation Frozen tumor mass were dissociated using homogenizer RNA was isolated using PureLink RNA Mini Kit from Invitrogen At leat 3ug of total RNA was used for the construction of sequencing libraries The RNAseq libraries were prepared with Illumina's 'TruSeq Stranded mRNAseq Sample Prep kit' (Illumina). Read 1 aligns to the ANTISENSE strand.