GSM8375432: UW228-3 cells cultured in normal media, replicate 2; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol Reagent (ThermoFisher, 15596018) and Direct-zol RNA extraction kit (Zymo Research, R2052) according to manufacturer's instructions. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers according to Novogene's workflow.