GSM7780231: Perio 4; Homo sapiens; RNA-Seq
Human gingival tissues were placed in Miltenyi M tubes (Miltenyi Biotec, 130-096-335) containing 1ml of Trizol reagent (Life Technologies, 15596018). Tubes were placed in Miltenyi gentlemacs and RNA 02.01 program was run. Samples were removed from M tube, placed in a 1.5 ml RNA-DNA free tube, and continue with Trizol RNA isolation protocol.” Library preparation was performed according to the manufacturer's instructions for the NEBNext Ultra Directional kit. The libraries were then pooled and sequenced using 75bp single end red mode on a NextSeq 500 sequencer (Illumina).