RNA-seq of B16F10 cell line
Total RNA was extracted using the Qiagen RNAeasy Mini Kit and RNA-seq libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina. mRNA was isolated from total RNA using oligo-dT magnetic beads. After fragmentation of the mRNA, a cDNA synthesis was performed. This was used for ligation with the sequencing adapters and PCR amplicification of the resulting product.