Intermittent childhood hyperlipidaemia alters resident arterial macrophages and accelerates atherosclerosis
Intermittent childhood hyperlipidaemia alters resident arterial macrophages and accelerates atherosclerosis
Peritoneal macrophages were obtained by intraperitoneal injection of cold PBS from Ldlr−/− mice reconstituted with Lyz2Cre+/− Nrp1flox/flox or Lyz2Cre+/− Nrp1+/+ bone marrow. Peritoneal exudates were allowed to adhere to the 6-well plates by culturing them for 2 hrs at 37°C. Animals were perfused with cold PBS to remove any trace of blood. Aortas were harvested and cleaned of contaminated tissues under a dissection microscope 16 wks mouse continuous high fat diet with Nrp1 WT vs KO Align to GRCm39 reference genome using star (version 2.6.1d) RNA extraction and residual DNA removal upon DNase digestion was performed with the RNeasy mini kit (Qiagen) according to the manufacturer's instructions. After quality check with Agilent Bioanalyser 2100 system, total RNA was made into sequencing libraries. Takara's SMART-Seq v4 Ultra Low Input RNA Kit.