GSM8019040: Control_male_pooled_ATAC; Mus musculus; ATAC-seq
For snMultiomic: L4-L6 DRGs from mice were collected at 14 days after CCI injury or without injury, and then immediately put on dry ice. Nuclei were extracted from frozen DRG according to a published protocal by the Allen Institute for Brain Science. Pooled nuclei suspension were used for snMultiomic sequencing. For smartseq3, all DRGs from mice were collected and immediately put on dry ice. Nuclei were extracted from frozen DRG according to a published protocal by the Allen Institute for Brain Science. Nuclei suspension was incubated with anti-NeuN PE conjugated antibody and DAPI. Single NeuN+ and DAPI+ nuclei were sorted into each well of a 384 well plate RNA-for smartseq3 sequencing. Library was performed according to the manufacter's instructions (Single Cell Multiome ATAC + Gene Expression protocal, 10x Genomics) 10x snMultiomics