RNA-seq rep2 of Bc2X
Total RNA from leaves was isolated using TRIzol reagent (Invitrogen) following the manufacturers instructions. The purity of the extracted RNA was detected by spectrophotometer (NanoDrop 2000&8000), and the concentration and integrity of RNA were detected by bioanalyzer(Agilent 2100). Three independent biologicalreplicates were performed. RNA-seq libraries were constructed for each group with three biological replicates. After the library building was completed, using the Agilent 2100 Bioanalyzer scope of library insertion pieces for testing, the concentration of the library of qualified quality inspection. Sequencing was performed using an Illumina platform (NovaSeq) at Igenecode Company (Beijing, China), and paired reads with an average length of 150 bp were generated.