Genome Sequence of BTV13: OBP Live Attenuated Virus
Total RNA extracted using Tri Reagent (LS) (Sigma) as described by the manufacturer. Random hexamers were used for cDNA synthesis and full-length amplification of cDNA was performed. Libraries were prepared using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs) and size selection completed using AMPure XP Beads (Beckman Coulter).  Sequencing was performed on the NextSeq 500 instrument using a NextSeq 500/550 Mid Output Kit v2.5 (300 Cycles).