RNA seq of Anomotaenia brevis, SMARTER amplified: whole cysticercoid
The RNA was isolated using the same protocol as the fat body samples and samples were sent to Novogene for RNA amplification using the SMARTer kit and RNA sequencing. None of our cestode samples had a lower RNA yield than 9ng, meaning we can assume that the amplification was reliable, as SMARTer amplification only becomes less reliable with an RNA yield below 1ng (Palomares et al., 2019). Library preparation and sequencing were done in the same way as described above. We sequenced a minimum of 9Gb per sample, resulting in around 30 million paired-end 150bp reads.