GSM7968663: multiplexed samples; Homo sapiens; RNA-Seq
Cells were resuspended in stain buffer, and each donor was labelled with a Sample Tag from the Human Single-Cell Multiplexing Kit. After washing, donors were pooled and stained with the BD AbSeq Immune Discovery Panel (BD Biosciences). Next, cells were loaded into a BD Rhapsody cartridge and checked for viability by Calcein AM (ThermoFisher) and DRAQ7 (BD Biosciences) in the BD Rhapsody™ Scanner. Cells were captured in the microwells of the cartridge and cDNA and exonuclease treatment was performed using the Rhapsody™ cDNA kit according to manufacturer's instruction Libraries were prepared using the BD Rhapsody™ WTA Amplification kit according to manufacturer's instruction