16S_day 115 oxic microcosm_1
DNA extracted from microcosm set up with waters from the Caspian Sea, collected at 25m to represent oxic conditions. Experiment microcosms contained a 60ppm amendment of native crude oil and was destructively sampled on day 115. The 16S gene was targeted by PCR amplification using 515F and 806R universal primers. Samples were multiplexed with unique barcodes for sequencing.