GSM8075614: mouse neocortex, Smad1, replicate2; Mus musculus; ChIP-Seq
Cells were fixed with 1.5% formaldehyde for 20 minutes. After quenching and cell lysis, chromatin was digested by using MNase for 20 minutes at 37 C. Libraries were generated using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (New England Biolabs, #E7645L) according to the manufacturer's instructions and PCR amplified.