GSM8018848: PC3shTIMP1_Veh1; Homo sapiens; RNA-Seq
RNAs were prepared using RNeasy Mini Kit (Qiagen, 74106), and DNA contaminations were removed with RNase-Free DNase (Qiagen, 79254). NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs Inc.) was used with the NEBNext Multiplex Oligos for Illumina (New England BioLabs Inc.) and NEBNext® Poly(A) mRNA Magnetic Isolation Module for cDNA synthesis; barcode sequences were also included. The sequencing of the prepools was carried out by exploiting the NextSeq2000 (Illumina, San Diego, CA, USA) with the P2 reagents kit V3 (100 cycles; Illumina). Samples were processed starting from stranded, single-ended 120bp-long sequencing reads with the P2 reagents kit V3 (100 cycles; Illumina). Samples were processed starting from stranded, single-ended 120bp-long sequencing reads