GSM7745677: GIST-T1, control, H3K27ac, rep2; Homo sapiens; ChIP-Seq
For ChIP-seq, cultured cells were fixed in 1% formaldehyde and snap frozen in liquid nitrogen and stored at -80ºC for at least overnight. Sonication of cultured cells was calibrated such that DNA was sheared to between 400bp and 2000bp. CTCF was immunoprecipitated with a monoclonal rabbit CTCF antibody, clone D31H2 (Cell Signaling 3418). H3K27ac was immunoprecipitated with a polyclonal rabbit H3K27ac antibody from Active Motif (cat 39133).  H3K27me3 was immunoprecipitated with a polyclonal rabbit H3K27me3 antibody, clone C36B11 (Cell Signaling 9733). ChIP DNA was used to generate sequencing libraries by NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB).