GSM7082462: siGL2 rep1; Homo sapiens; ncRNA-Seq
RNA was extracted using Trizol following manufacturer's protocol. Small RNA-seq library preparation was similar to previously described (Su et al. 2019) with addition of spike-in. Briefly, 1 ul (500 ul total volume) of Qiaseq miRNA library QC spike-in (Qiagen #331535) were mixed with 1 ug total RNAs before library preparation. The resulting libraries were size selected for 15-50 nt inserts, and pooled for sequencing on Illumina NextSeq500.