GSM6926573: HDP-005589; Mus musculus; RNA-Seq
Mice were sacrificed at week 1,2 and 6 (folic acid treated) or weeks 2 and 6 (controls). The sacrifices took place from 8:30 a.m. until 12 a.m., with isoflurane anesthesia followed by a complete blood draw (~1 mL) from the vena cava, followed by perfusion with phosphate-buffered saline. The left kidney and other organs were flash-frozen in liquid nitrogen and stored at -80°C until processing.For mRNA, liver tissue were crushed in liquid nitrogen and then 10 mg of tissues were suspended in TRIzol (Invitrogen) and homogenized with stainless steel beads using a TissueLyser II (Qiagen) at 30 Hz for 2 min. RNA was extracted and purified using Direct-zol-96 RNA kits (Zymo Research). mRNA concentration was measured for all samples. All samples passed a quality check of purity (NanoDrop) and fragmentation (FragmentAnalyzer). RNA libraries were prepared for sequencing using SMARTER mRNA-Seq Library Prep Kit standard protocols.