GSM8385871: Reduction Mammoplasty; Homo sapiens; RNA-Seq
Single nuclei were isolated from approximately 50 mg of frozen tissue for each tumor sample and 100 mg for each peri-tumoral and RM sample by Singleron Biotechnologies using the GEXSCOPE® Single Nucleus RNAseq kit. Single nuclei were partitioned into the SCOPE-microwell chip isolated mRNA from each nucleus was hybridized to unique nucleus-identifying tags barcode sequences on the bead in the same well. The beads containing the hybridized mRNA were collected and reverse transcribed into cDNA. The resulting cDNA was then amplified by PCR, fragmented, ligated to adapters, and PCR amplified to construct a sequencing library suitable for the Illumina sequencing platform. snRNA-seq