RNA-seq of CD56+ NK cells from juvenile systemic lupus erythematosus (JSLE) patients and healthy controls
RNA-seq of CD56+ NK cells from juvenile systemic lupus erythematosus (JSLE) patients and healthy controls
Peripheral blood was obtained by venipuncture, followed by PBMC isolation via Ficoll gradient.  Cells were frozen in liquid nitrogen until use. Thawed PBMCs were sorted using FACS to select CD3-CD14-CD56+ NK cells. PicoPure RNA extraction kit as per manufacturer's protocol NEBNextÂ® Single Cell/Low Input RNA Library Prep Kit for Illumina