scRNAseq of Human: Placental Decidua
CD45+ sorted cells were counted in triplicates and resuspended in PBS with 0.04% BSA in a final concentration of 1200 cells/uL. Cells were immediately loaded in the 10X Genomics Chromium with a loading target of 17,600 cells. Libraries were generated using the V3 chemistry (gene expression) and Single Cell 3 Feature Barcode Library Kit per manufacturers instructions (10X Genomics, Pleasanton CA). Libraries were sequenced on Illumina NovaSeq with a sequencing target of 50,000 gene expression reads.