GSM7966470: EmT_cl14_H3K27me3_experiment1_rep2; Mus musculus; ChIP-Seq
Samples were fixed and lysed as described before (Schmidt et al., 2009). The chromatin extracts were sonicated for 6 cycles of 30 sec on, 30 sec off using a Diagenode Bioruptor Pico. Immunoprecipitated DNA was processed for library preparation KAPA HyperPrep Kit (KAPA Biosystems) using 5ng DNA starting material. Sequencing was performed using an Illumina NextSeq 500, 42-bp paired-end reads were generated.