ATACseq of mus musculus: HPC5 cell line
ATAC-seq was performed following the Fast-ATAC protocol5. Briefly, 10,000 of HPC5 cells were treated with transposition mix: 1) 22 uL nuclease-free water; 2) 25 uL 2  TD Buffer; 3) 2.5 uL TDE1 transposase; 4) 0.5 uL 1% Digitonin (added right before removing supernatant from cell pellets), followed by incubation at 37C on thermomixter at 300 rpm for 30 minutes. DNA was purified by using the MinElute Reaction Cleanup kit (Qiagen, 28204), and amplified for 5 cycles with the following mix: 10 uL transposed DNA, 2.2 uL nuclease-free water; 6.25 uL 10 uM barcoded Nextera primer-F; 6.25 uL 10 uM barcoded Nextera primer-R; 0.3 uL 100  SYBR Green I (Invitrogen, S-7563); 25 uL NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541). PCR products were subsequently re-amplified for 6 additional PCR cycles and subjected to purification using SPRIselect beads (Beckman Coulter, B23317).