GSM7745753: siRGS1 Treg Donor 3; Homo sapiens; RNA-Seq
RNA was purified from isolated cells using the RNeasy Mini Kit (Qiagen, Cat. # 74104) with QIAshredder (Qiagen, Cat. #79654) homogenization according to the manufacturer's instructions. Samples for RNA-seq underwent an additional purification using the RNase-free DNase set (Qiagen, Cat. #79254). RNA concentration and purity were assessed using NanoDrop 8000 (Thermo Scientific) and RNA-seq samples were also assessed using the Qubit™ 1X dsDNA HS Assay Kit (Invitrogen, Cat. # Q33230). RNA-seq libraries were prepared using the Illumina Stranded mRNA Prep Ligation kit (Illumina, Cat. #20040532) according to manufacturer's recommendation