16S_day 0 hypoxic microcosm_1
DNA extracted from microcosm set up with waters from the Caspian Sea, collected at 350m to represent hypoxic conditions. Experiment microcosms contained a 60ppm amendment of native crude oil and was destructively sampled on day 0. The 16S gene was targeted by PCR amplification using 515F and 806R universal primers. Samples were multiplexed with unique barcodes for sequencing.