16S amplicon NGS  PLA12_N0164_16Sv3v4_PLA Run 1_anaerbic digestate
Samples for microbial analyses were collected before (Inoculum) and after (Control, Cellulose, PLA and SBS) each run. To recover the microbial communities, digestates were pelleted by centrifugation at 13,000 rpm for 20 min at 4C. All samples were stored at -80C prior to DNA extraction. Pellets (~0.060 g) were extracted using a DNeasy PowerSoil Pro Kit (Qiagen, Germany) according to manufacturers guidance. The yield and purity (A260/A280 and A260/A230) of the extracted DNA was quantified on a Nanodrop 1000 spectrophotometer (Thermo Fisher Scientific) while eventual fragmentation was determined through gel electrophoresis 1% (w/v) 1TAE agarose gels. Replicates (n=3) were pulled together to minimize extraction variability. DNA was stored at 80 C until analysis. Illumina sequencing was performed on all samples for prokaryotic communities at Stab Vida Lda (Lisbon, Portugal). For bacteria, the 16S rRNA gene was selected and amplified using primers 341F and 785R (Klindworth et al., 2013). The generated DNA libraries were sequenced with MiSeq Reagent Kit Nano in the lllumina MiSeq platform, using 300bp paired-end sequencing reads.