GSM8075109: EO771 cells, sgPcsk9, shLrp1-3, biol rep1; Mus musculus; RNA-Seq
Total RNA from cells cultured in quadruplicate was isolated with the Total RNA Purification Kit (Norgen Biotek, 17200) with 5ul/ml of RNasin Plus (Promega, N2615) and 10ul/ml of 2-mercaptoethanol (Sigma-Aldrich, M6250) added to buffer RL. Libraries were constructed from 1μg of total RNAs using the TruSeq RNA Library Prep Kit (Illumina, RS-122-2001)