GSM7702816: vHip,Resilience,Rep2; Mus musculus; RNA-Seq
Following nuclei isolation, Ten thousand GFP+ or GFP- FANS-sorted nuclei were collected in 100 μL of RLT buffer followed by flash freezing. RNA was purified using the RNeasy Micro kit (Qiagen 74004, Hilden, Germany) following the manufacturer's instructions. For the nuclear RNA-seq library preparation, we used  the Ovation® SoLo RNA-Seq System following NuGEN's standard protocol (NuGEN M01406v2, Redwood City, CA, USA).