GSM7527545: ATAC-Seq LNCaPabl_Vehicle_2; Homo sapiens; ATAC-seq
Cells (1x105) were washed in cold PBS and lysed in cold lysis buffer (10 mM Tris-HCl, pH = 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630). The transposition reaction (Illumina) was performed at 42C for 45 min. After purification of the DNA with the MinElute PCR purification kit (QIAGEN) After purification of the DNA with the MinElute PCR purification kit (QIAGEN), the material was amplified for 5 cycles using NEB Next Ultra DNA Library Prep (New England BioLabs), and size was verified on Tapestation. Normalized libraries were sequenced on a HiSeq2500 1T in a 50bp/50bp Paired-end run at the Genome Technology Center at NYU, using the TruSeq SBS Kit v3 (Illumina).