RNAseq of rat brain cortex
The University of Wisconsin-Madison Biotechnology Center's Gene Expression Center Core Facility (research resource identifier [RRID]: SCR_017757) conducted quality control assessments, RNA library preparation, sequencing, and read demultiplexing. Library construction involved poly(A) enrichment using the Illumina TruSeq stranded mRNA kit. Double stranded cDNA was purified by AMPure Xp beads and frozen at -20C. Library quantification was performed on Agilent Biomek Synergy H1 Plate Reader with Picogreen reagent and final libraries assayed on Agilent 4200 Tapestation with HS D1000 ScreenTapes. Following quality control, samples were sequenced on the NovaSeq S4 platform (Illumina) with a 2  150-bp configuration. On average, each sample generated approximately 50 million paired-end reads, with an average read length of 450 base pairs.