Illumina library for WGS of Mesocentrotus franciscanus: adult male gonad
gDNA from gonad tissue was randomly shears on a Covaris M220 with a targeted size of 500bp. Fragmented DNA was prepared for sequencing using the KAPA HyperPrep DNA kit. A double-sided size selection was done with PCRclean DX beads (Aline Biosciences) to target inserts ~550 bp in length. Seqencing was performed on a v2.5 2x251 NextSeq 500 run.