GSM7866879: Liver, Vehicle 8 w, Rep 9 [16245]; Mus musculus; RNA-Seq
Liver tissue (Chow mice, GAN DIO-NASH mice, and chow-reversal mice) were collected and snap-frozen in liquid nitrogen. RNA was isolated using the NucleoSpin® kit (MACHEREY-NAGEL). RNA sequence libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, USA) and sequenced on the NextSeq 500 (Illumina) with NextSeq 500/550 High Output Kit V2 (75 CYS, Illumina, San Diego, USA).