16S V3-V4 amplicon control
DNA was isolated using the MO BIO PowerSoil DNA isolation kit (Mo BIO Laboratories Inc, Carlsbad, CA) and adapted for swab use during bead beating and standard protocol for hand samples (Kampf et al., 2006). The V3-V4 amplicon was amplified and standard Illumina library prep protocol performed and sequenced on Illumina MiSeq.