GSM7042892: ∆staRPZnstaR strain, +Zn, +novobiocin, rep1; Streptococcus pneumoniae; RNA-Seq
RNA (about 5 µg) was obtained from samples containing 2-4E8 cells using the RNeasy mini kit (Quiagen). It was depleted of ribosomal RNA using the Ribo-Zero (Bacteria) Magnetic Kit (Illumina). Libraries for RNA-Seq were prepared from 1 µg RNA using the ScriptSeq v2 RNA-Seq system (Illumina).