RNA-seq comparison of leukemic bone marrow (BM) cells isolated from WT, Ppm1d T/+ and healthy control
RNA-seq comparison of leukemic bone marrow (BM) cells isolated from WT, Ppm1d T/+ and healthy control
Long-term HSCs (LT-HSCs sorted from 12-week-old WT and Ppm1DT/+ mice using Influx instrument (BD Biosciences). RNA was isolated from c-Kit+ cells isolated from the leukemic animals using KAPA mRNA HyperPrep Kit (Kapa Biosystems). Library was prepared using Illuminaâ€™s KAPA mRNA Hyperprep Kit as per manufactures instructio