GSM7866813: 496liver; Mus musculus; RNA-Seq
Mice were monitored in the calorimetry chambers with recordings at 4-min intervals. After 30 min, ambient temperature of the chambers was lowered from 22°C to 4°C, and remained 4°C for 6 h. The mice were then quickly perfused with saline solution. Epididymal WAT, BAT, thigh skeletal muscle, and liver samples were collected and snap frozen in liquid nitrogen. RNA libraries were prepared for sequencing using standard Illumina protocols