JPN02_uppermost_leaflets_rep3
RNA extraction from Melia azedarach plants was performed using the MacKenzie-modified RNeasy Plant Mini Kit (Qiagen) protocol, with DNAase (Promega) treatment, performed on column. High-throughput Illumina stranded RNA libraries (150bp, paired end) of each of the 28 samples were generated, which were multiplexed and sequenced over two lanes of a HiSeq 4000 instrument (Illumina). This generated over 635 million paired end reads (an average of 91 million per tissue).