RNAseq of MDR Acinetobacter baumannii
). RNAs concentration were determined by using NanoDropOne spectrophotometer (Thermo Fisher) and its quality assessed with the TapeStation 4200 (Agilent Technologies). Indexed libraries were prepared from 150 ng/ea purified RNA with Stranded Total RNA Prep with Ribo-Zero Plus (Illumina) according to the manufacturers instructions. Libraries were quantified using the TapeStation 4200 (Agilent Technologies) and and Qubit fluorometer (Invitrogen Co.), then pooled such that each index-tagged sample was present in equimolar amounts. The pooled samples were subject to cluster generation and sequencing using an Illumina NovaSeq6000 System (Illumina) in a 2x100 paired-end format.