RNA-Seq of mus musculus: oocyte
Samples from different stages were collected from WT, Mtr4fl/fl and Mtr4fl/fl;Zp3-Cre mice with 10 oocytes per sample. Each sample was directly lysed with 2 l lysis buffer (0.2% Triton X-100 and RNase inhibitor) and 0.2 l 1:1000 diluted ERCC). RNA in cell lysis buffer were reversed according to the published Smart-seq2 method. Qsep analysis of cDNA quality was conducted to confirm the main peaks of cDNA library pool to be more than 1000bp. Full-length cDNA library was fragmented using the TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme, TD503). DNA fragments were selected and the final sequencing libraries were constructed from 300-400 bp of amplified cDNA. Sequencing data was extracted on the Illumina HiSeq X Ten platform in the 150 bp paired-end mode.