GSM7706591: sample DLT; Drosophila melanogaster; RNA-Seq
Trachea were manually dissected on the dissection dish with ice-cold Schneider's medium described  Chen (2016).Then trachea was transferred into a new petri dish filled with ice-cold Schneider's medium on ice after dissection. The dissection work was managed within 1 hour. Single-cell gene expression libraries were generated with theChromium™ Single Cell 3' Library and Gel Bead Kit v2 (10X Genomics, Pleasanton, California, USA) according to the manufacturers manual.