Early-onset MCC genome sequencing
Libraries were prepared with the Illumina DNA PCR-free Library Prep kit (#20041795) according to the manufacturer's instructions (#1000000086922 v03). Genomic DNA (500ng) was tagmented using Bead-Linked Transposomes PCR-Free (BLT-PF), followed by a clean-up and ligation of Index 1 (i7) and Index 2 (i5) adapters. Final libraries were pooled and quantified with the Qubit ssDNA assay kit (#Q10212). Sequencing was performed on the Illumina NovaSeq6000 using v1.0 NovaSeq reagent kit with VP10 custom sequencing primer, set for a paired-end run of 151 cycles, with 10 bp index reads (Read 1, i5 index read, i7 index read, Read 2; 151, 10, 10, 151) on S2 or S4 flow cells, following Illumina recommendations.