GSM8528640: WT_RNA_2; Mus musculus; RNA-Seq
Primary mouse neurospheres were lysed with TRIzol (Thermo Fisher Scientific) and snap-frozen in liquid nitrogen after repeated blowing with pipettes until no clumps of cells could be seen. Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA, Catalog #: E7530L). The library fragments were purified with AMPure XP system (Beverly, USA). PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent 5400 system (Agilent, USA).