Metagenome Soil+Sludge 2
Soil DNA was extracted with the DNeasy PowerSoil Pro Kit. Genomic DNA was then fragmented by acoustic shearing with a Covaris S220 instrument. Fragmented DNA was cleaned up and end repaired. Adapters were ligated after adenylation of the 3ends followed by enrichment by limited cycle PCR. DNA libraries were validated using a NGS Kit on the Agilent 5300 Fragment Analyzer and quantified by using Qubit 4.0 Fluorometer. The DNA libraries were multiplexed and loaded on the flow cell on the Illumina NovaSeq 6000 instrument.