GSM7867570: TLN-1; Homo sapiens; RNA-Seq
RNA was extracted using Rneasy mini plus kit (Qiagen).  tRNAs were purified from total RNA and m1A&m3C demethylated then partially hydrolyzed according to the Hydro-tRNAseq method. Fragments of ~19-35 nt for partially hydrolyzed and re-phosphorylated tRNAs were converted to small RNA sequencing libraries using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® kit