GSM8338109: CAG, small RNA-seq, rep2; Homo sapiens; miRNA-Seq
Cells were scraped from 70% confluent 10 cm plates in ice-cold PBS, pelleted, and Trizol was added. Total RNA was isolated with Trizol, with an extra chloroform extraction to remove residual phenol and addition of glyco-blue as a carrier to promote RNA precipitation. Small RNA-seq libraries were prepared by the RNA Sequencing Core and the Transcriptional Regulation and Expression Facility at Cornell University with the NEBNext Small RNA Library Prep Kit.