GSM8125086: sample80_nocrRNA; Homo sapiens; RNA-Seq
~200k-1 million cells were harvested, resuspended in 300ul RNA Lysis Buffer (Zymo cat# R1060), and stored at -70deg. C until further processing for RNA isolation using the Quick-RNA Miniprep Kit (Zymo cat #R1055). 3' RNA-seq was batch processed together with samples unrelated to this study using a QuantSeq-Pool Sample-Barcoded 3′ mRNA-Seq Library Prep Kit for Illumina (Lexogen cat#139) in accordance with the manufacturer's instructions. 10 ng of each purified input RNA was used for first strand cDNA synthesis with an oligo(dT) primer containing a sample barcode and a unique molecular identifier. Subsequently, barcoded samples were pooled and used for second strand synthesis and library amplification.