GSM6615899: RNA-Seq Dual-specificity Aptamer_Wnt+_Rep3; Homo sapiens; RNA-Seq
At 24 hr post-transfection, medium was discarded, and cells were lysed using 1 mL of TRIzol Reagent per culture dish. Lysates were mixed by pipetting, transferred into a Phasemaker tube (Invitrogen A33248) and incubated at room temperature for 5 min. The aqueous phase was separated by adding 200 μL chloroform, thoroughly vertexing and spinning at 12,000 rcf for 15 min at 4 °C. The upper aqueous layer was carefully collected without toughing the other layers. RNA was precipitated with isopropanol, washed with 70% ethanol, dissolved in nuclease-free water, and submitted to GENEWIZ (South Plainfield, NJ, USA) for sequencing. Library construction was performed by GENEWIZ.