RNA-sequencing of Vitis riparia (accession PI 588710) control leaf tissue for replicate 3
RNA was extracted using a Spectrum Plant Total RNA Kit from Sigma-Aldrich (STRN250), as well as the On-Column DNase I Digestion Set from Sigma-Aldrich (DNASE70-1SET) to remove DNA from the samples. RNA-seq libraries were prepared using the Illumina Stranded mRNA Library Preparation, Ligation Kit with IDT and Illumina Unique Dual Index adapters following the manufacturers recommendations, except half volume reactions were used. The libraries were pooled together and sequenced on a NovaSeq6000 using one lane of an Illumina S4 flow cell in a 2x150bp paired-end format and a NovaSeq v1.5 reagent kit (300 cycles). Base calling was done by Illumina Real Time Analysis (RTA) v3.4.4. The output of RTA was demultiplexed and converted to FastQ format with Illumina Bcl2fastq v2.20.0.