GSM8002116: HashTagLibrary; Mus musculus; RNA-Seq
Splenocytes from co-transplanted mice were harvested 12 days after transplantation. Ex vivo progenies were labeled with Hashtag-Abs (TotalSeq™-B0301-4 anti-mouse Hashtag 1-4 Antibody, BioLegend, catalog: 155831, 155833, 155835, 155837). Cells were sorted for Tom+/7AAD- (BioLegend; catalog 420404) with an Aria III fluorescence-activated cell sorter (BD). The single cell suspension was loaded onto a well on a 10X Chromium Controller (10x Genomics). Library was performed according to the manufacter's instructions (Chromium Next GEM Single Cell 3'v3.1 + Cell Surface Protein (Dual Index), 10x Genomics). Cellular mRNA and hashtag oligos (HTO) were reverse-transcribed and indexed with a shared cellular barcode. Indexed cDNAs were amplified by PCR according to the Chromium Single Cell 3' v3.1 protocol. scRNA-seq (gene expression with feature barcoding