RNA-seq of rat RBL-2H3 cell line cultured at 29 C for 2 hours
Total RNA was extracted from the sample using the Qiagen RNA minikit (QiagenGermany). Nucleic acid quantification was performed using Qubit 4 (Thermo Fisher Scientific). Next-generation sequencing library construction used the NexteraXT kit (Illumina, USA) according the manufacturers instruction. The libraries were sequenced on Illumina NextSeq550 using paired-end mode.