GSM7527438: SKBR3, siCTRL, rep2; Homo sapiens; RNA-Seq
Total RNA was extracted using RNeasy Mini Kits (Qiagen). 100 ng of total RNA was used for the construction of sequencing libraries. RNA-seq libraries were prepared using an Ion AmpliSeq Transcriptome Human Gene Expression kit (Thermo Fisher Scientific) according to the manufacturer's instructions.