WGS of Sallmonella Paratyphi A : Clinical isolates
The sample was quantified using Qubit DNA HS Assay (Invitrogen, Cat#Q32854).The DNA sample is further taken for DNA library preparation using Twist Library Preparation Enzymatic Fragmentation (EF) Kit (TWIST Biosciences, Cat #: 104175).Prepared library was quantified using Qubit HS Assay (Invitrogen,Cat#Q32854). The obtained library wes pooled and diluted to final optimal loading concentration. The pooled library was then loaded on to Illumina NovaSeq X plus to generate 150bp paired-end reads/sample.