RNA-Seq of mus musculus: adult female whole blood
Whole blood samples were collected from mice 28 days after the initial immunization. Total RNAs were extracted from the blood using Trizol Reagent (cat. NO 15596026, Invitrogen). 2g total RNAs were used for stranded RNA  sequencing library preparation using KC-DigitalTM Stranded mRNA Library Prep Kit 464 for Illumina (Catalog NO. DR08502, Wuhan Seqhealth Co., Ltd. China) following the manufacturers instruction. The library products corresponding to 200-500 bps were  enriched, quantified and finally sequenced on DNBSEQ-T7 sequencer (MGI Tech Co., 467  Ltd. China) with PE150 model mRNA sequencing Statistical analysis