16S-rRNA-Seq of rainbow trout: gill mucus
DNA sample of 30 ng was amplified with 16S rRNA fusion primers. The forward primer was: 515F, GTGCCAGCMGCCGCGGTAA; The reverse primer was: 806R, GGACTACHVGGGTWTCTAAT. After purification with Agencourt AMPureXP beads (Beckman Coulter, Brea, US) qualification with an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, US), the amplicons were sequenced on the Illumina Hiseq PE250 platform at the V4 region