Metagenom of biogas fermenter
Preparation of the 16S metagenomic sequencing library for bacteria was performed using a primer pair that targeted the V3-V4 region while the archaeal metagenomic library was constructed using the primers 21F_Arch and 516R_Arch (both Macrogen Inc. primer set). Both libraries were created with Herculase II Fusion DNA Polymerase Nextera XT Index Kit V2. Sequencing was performed by Macrogen Inc. (Seoul, Korea) with MiSeq - 300 PE, which was conducted with a reading length of 301 bp and FastQC quality control.