GSM8338103: CAG, RNA-seq, rep2; Homo sapiens; RNA-Seq
Cells were scraped from 70% confluent 10 cm plates in ice-cold PBS, pelleted, and Trizol was added. Total RNA was isolated with Trizol, with an extra chloroform extraction to remove residual phenol and addition of glyco-blue as a carrier to promote RNA precipitation. Directional RNA-seq libraries were prepared for each sample using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs), with initial polyA+ isolation, by the RNA Sequencing Core and the Transcriptional Regulation and Expression Facility at Cornell University.