Replicate 2 of IT-scC&T-seq of multiple histone marks in multiple cell lines
Library Strategy: CUT&Tag. Library Selection: CUT&Tag. For each indexed tagmentation reaction, combinations of cell lines (H1, HEK293T, and H1-derived MSCs) and antibodies (H3K4me3, H3K27me3, H3K36me3, CTCF) were used. Nuclei were extracted, tagged with indexed pA-Tn5 transposase, and sorted into two 384-well plates. PCR amplification was performed in two rounds:first add well barcode, then product from all wells are pooled to add a plate-specific barcode. Libraries were all pooled purified, and sequenced after quality control.