GSM8075332: coD14_KO1_RNA_rep2; Homo sapiens; RNA-Seq
Tissues were dissolved in TRI reagent, mixed with chloroform, and centrifuged. The recovered supernatant was applied to RNeasy column to purify the RNA according to the manual. One microgram total RNA was used for next generation sequencing library preparation according to the manufacturer's protocol (VAHTS® Universal V8 RNA-seq Library Prep Kit for Illumina, Vazyme #NR605-01)