RNA-Seq of mus musculus
For each frozen sample, FASTQ files and manually aligned histology images were analyzed with Space Ranger 1.1.0 which uses theSTAR genome aligner version v.2.5.1b and the human reference genome hg38 (refdata-gex-GRCh38-2020). An average of 3700 mediangenes per spot was measured, with one batch having poorer quality metrics (tumors id: c_2, c_5, c_23, c_57) with median genesper spots ranging from 875 to 2078 median genes per spot