GSM8423686: whole kidney, control (Pkd1flox/flox.Tet-O-Cre), sample 3; Mus musculus; RNA-Seq
Total RNAs were obtained from whole snap-frozen mouse kidneys using RNeasy Mini Kit (Qiagen). RNA quantification and quality was performed using Nanodrop photometer. Purification of poly-A containing mRNA molecules, mRNA fragmentation, randomly primed cDNA synthesis, adapter ligation and adapter-specific PCR.