GSM6503224: Glomeruli MCTO rep2 [LAB-177-13]; Mus musculus; RNA-Seq
RNA-seq was applied for transcriptome analysis. RNA was extracted from glomeruli from 3 control (Mafb MCTO/ MCTO) and 3 wild-type mice. Briefly, total RNA was isolated using TRIZOL reagent (ThermoFisher Scientific). RNA quality was controlled using an RNA 6000 Pico kit (Agilent, Santa Clara, CA, USA). A total amount of 250 ng total RNA was used for RNA-seq library preparation using the NEBNext rRNA Depletion Kit and NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs, Ipswich, MA, USA); 2×36 base paired-end sequencing was performed with NextSeq500 (Illumina, San Diego, CA) by Tsukuba i-Laboratory LLP (Tsukuba, Ibaraki, Japan). RNA libraries were prepared for sequencing using standard Illumina protocols