GSM7966932: UCD1172CR_2; Homo sapiens; RNA-Seq
Total cellular RNA was isolated from the prostate cancer cell lines using the RNeasy Mini Kt (Qiagen). Indexed, stranded mRNA-seq libraries were prepared from total RNA (100 ng) according to standarding protocols for end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification.