GSM8001942: MutuI_Ctrl_Day1_1; Homo sapiens; RNA-Seq
Total RNA was isolated using a RNeasy Mini kit with in-column genomic DNA digestion. To construct indexed libraries, 1 μg of total RNA was used for polyA mRNA purification, using the NEBNext Poly(A) mRNA Magnetic Isolation Module, followed by library preparation using the NEBNext Ultra RNA Library Prep with Sample Purification Beads (#E7760L, New England Biolabs).