GSM8124894: WT_2; Synechocystis sp. PCC 6803; ChIP-Seq
The samples were cross-linked with formaldehyde at a final concentration of 1%, and the cross-linking reaction was terminated with glycine. Ultranic interruption of the DNA-protein complexes formed by cross-linking; The target protein antibody is added to form an antibody-target protein-DNA complex; This complex was precipitated using Protein G beads, specifically enriching DNA fragments bound to the protein of interest; Multiple washes to remove non-specifically bound chromatin and purify the complex; Unraveling cross-links and purifying DNA fragments; q-PCR or sequencing analysis. Immunoprecipitated DNA was used to construct sequencing libraries following the protocol provided by the I NEXTFLEX® ChIP-Seq Library Prep Kit for Illumina® Sequencing（NOVA-5143-02，Bioo Scientific） and sequenced on Illumina Novaseq 6000 with PE 150 method