GSM6758686: FC00185: Control_22; Mus musculus; RNA-Seq
DRGs collected from Nav1.8-Cre R26CAG-floxStop-tdTomato mice were digested using 0.5 mg/mL liberase TL, 5 mg.mL dispase II, 0.33 mg/mL papain and 0.1mg/mL Dnase I in DMEM (with 2-ME). Samples were incubated for 20 min, mechanically agitated using glass Pasteur pipettes to prepare single cell suspension. tdTomato+ cells were single cell sorted using BD FACS Aria III into tubes containing 0.4uL of 10 x SingleCellProtect and immediiately frozen in LiqN2. Cell collection and sequencing was performed at four separate occasions from 6 naive Nav1.8-Cre R26CAG-floxStop-tdTomato mice . FC00029 contains cells from 2 mice (labelled blue and red), FC00185 contains samples from 1 mouse, FC00355 from 2 mice (Control1 and Control2) and FC00404 from 1 mouse (Control6). Library was generated as previously described (Tenno et al, 2017,  J Exp Med 214, 2933-2946. 10.1084/jem.20170729.) scRNA-seq using digital RNA sequencing method (Shiroguchi et al. 2012, Proc Natl Acad Sci U S A 109, 1347-1352. 10.1073/pnas.1118018109.)