GSM8214607: 1team-3; Homo sapiens; RNA-Seq
The extracted total RNA samples are subjected to agarose electrophoresis and Nanodrop quality inspection and quantification, followed by mRNA enrichment using oligo(dT) magnetic beads.1-2 ug of total RNA was used for the construction of sequencing libraries. Total RNA is first enriched for mRNA using the NEB Next® Poly(A) mRNA Magnetic Isolation Module (or rRNA is removed using the RiboZero Magnetic Gold Kit). The processed RNA product is then used to construct a library with the KAPA Stranded RNA-Seq Library Prep Kit (Illumina).