Deep Sequencing of Korean Jindo Dog Reveals Evolutionary Trajectory of Coat Color Variations
Deep Sequencing of Korean Jindo Dog Reveals Evolutionary Trajectory of Coat Color Variations
For whole genome sequencing, whole blood samples were collected from 113 Jindo dogs. The experiment was ethically approved by the National Institute of Animal Science, Rural Development Administration (approval no: NIAS2018-275). Total DNA was extracted from the trachea with TRIzol Reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer's protocol. DNA concentration was checked using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, USA). Individual libraries were generated using Illumina TruSeq™ DNA Sample Preparation Kit (Illumina, San Diego, CA, USA).