16S of feces
Feces were collected in a steril paper. To optimize DNA extraction, 50 ml of molecular grade water were added in the paper with the feces, homogenizeed using a Stomacher 400 Circulator for 2 minutes. The DNA was extracted using the Kingfisher equipment with the MagMAXmicrobiome Ultra kit (Thermo Fisher Scientific, Austin, TX, USA), according to the manufacturer's instructions