RNA-seq comparison of bone marrow stem cells isolated from two genotypes WT and Ppm1dT/+
RNA-seq comparison of bone marrow stem cells isolated from two genotypes WT and Ppm1dT/+
Long-term HSCs (LT-HSCs sorted from 12-week-old WT and Ppm1DT/+ mice using Influx instrument (BD Biosciences). RNA was isolated from c-Kit+ cells isolated from the genotypes using KAPA mRNA HyperPrep Kit (Kapa Biosystems). Library was prepared using Illuminaâ€™s KAPA mRNA Hyperprep Kit as per manufactures instruction