GSM6586016: mouse-muscle-cell-differentiating-8A-ChIP-input; Mus musculus; ChIP-Seq
Chromatin for ChIP was prepared as previously described(Kouwenhoven et al., 2010). ChIP assays were performed following a standard protocol (Novakovic et al., 2016) with minor modifications. Antibodies against H3K27ac (1.2 μg) was used in each ChIP assay. Resulted DNA fragments from four independent ChIP assays were purified and subjected to a ChIP-qPCR quality check. Afterward 5ng DNA fragments were pooled and proceeded on with library construction using KAPA Hyper Prep Kit according to the standard protocol. The prepared libraries were then sequenced using the NextSeq 500 (Illumina) according to standard Illumina protocols.