mRNA sequencing of Caenorhabditis elegans
Whole animal lysis; cDNA preparation from crude lysate by Mars-seq2; T7 amplification, fragmentation and Illumina sequencing adapter ligation; sequenced on NextSeq500 (60/8/0/15 bases); demultiplexing information can be found under https://github.com/nstroustrup/asynch-seq-2024