GSM8374127: NTM.4; Rattus norvegicus; RNA-Seq
Total RNA was isolated from tissues using the Magzol Reagent (Magen, China)according to the manufacturer's protocol.The quantity and integrity of RNA yield was assessed by using the K5500(Beijing Kaiao, China) and the Agilent 2200 TapeStation (Agilent Technologies, USA) separately. the mRNA was enriched by oligo dT according to instructions of NEB Next® Poly(A) mRNA Magnetic Isolation Module (NEB,USA). And then fragmented to approximately 200bp. Subsequently, the RNA fragments were subjected to first strand and second strand cDNA synthesis following by adaptor ligation and enrichment with a low-cycle according to instructions of  NEBNext® Ultra™ RNA Library Prep Kit for Illumina.