GSM8374962: RVM SYNs, Neonatal zymosan exposures postnatal days P12-P16 and reexposure on P29, S12; Rattus norvegicus; RNA-Seq
RVM tissues were removed on P30 from rats and immediately processed for synaptoneurosomes (SYNs) isolation. Total RNA was extracted from RVM SYNs preparation using Qiagen miRNeasy mini kit (Qiagen, Hilden, Germany) according to the manufacturers' instruction. The library was prepared for sequencing from 300 ng RNA of each sample using the Illumina standard total RNA prep ligation with ribo-zero plus library preparation kit (Illumina, San Diego, CA, USA). The quality and quantity of amplified libraries were evaluated using Qubit (Invitrogen, Carlsbad, CA, USA) and Agilent TapeStation high sensitivity D1000 Screen Tape. The average fragment length of the cDNA libraries was 330 bp. RNA-seq libraries were sequenced using paired-end and SP flow cell on Illumina NovaSeq 6000 instruments following the manufacturer's protocol.