Metagenomics of rat: intestinal content
The degree of DNA degradation and potential contamination were assessed using 1% agarose gel electrophoresis. DNA concentration was quantified utilizing the Qubit dsDNA Assay Kit with the Qubit 2.0 Fluorometer (Life Technologies, CA, USA). Samples with an OD value falling within the range of 1.8 to 2.0, and DNA concentrations exceeding 7 g, were selected for library construction.