GSM8019195: INTS12_R2; Homo sapiens; OTHER
TTchem-seq RNA preparation was performed as previously described (Gregersen et al., 2020). TTchem-seq libraries were prepared using the KAPA RNA HyperPrep Kit (Roche, 08098093702) with the fragmentation step omitted. 75 ng of nascent RNA was denatured at 65°C for 5 min. The rest of the procedure was performed following manufacturer's instructions, except that two SPRI bead purifications (Beckman Coulter, A63881) were carried out, with a bead-to-sample volume ratio of 0.95x and 1x respectively.