GSM8075725: bone marrow mesenchymal stem cell,WT3; Mus musculus; RNA-Seq
Total RNA was extracted using the TRIzol reagent according to the manufacturer's protocol. RNA purity and quantification were evaluated using the NanoDrop 2000 spectrophotometer (Thermo Scientific, USA). RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Libraries were prepared using TruSeq Stranded mRNA LTSample Prep Kit (Illumina, USA) and sequenced them on Illumina sequencing platform according to manufacturer's instructions.