GSM7461996: Clone13, Adult 3; Mus musculus; RNA-Seq
Total RNA was isolated with Trizol, with an extra chloroform extraction to remove residual phenol and addition of glyco-blue as a carrier to promote RNA precipitation. RNA concentration was determined with a Qubit and integrity assessed on a Fragment Analyzer. RNA-seq libraries were prepared from 10ng total RNA using the NEBNext Directional Ultra II RNA Library Prep Kit for Illumina (New England Biolabs), with initial [polyA+ isolation/rRNA depletion].