GSM8374402: UV8; Mus musculus; RNA-Seq
Female ICR SCID mice with or without s.c. HT1080 tumors were treated with vehicle while female ICR SCID mice with s.c. HT1080 tumors were treated with selpercatinib (30 mg/kg, once a day, oral gavage) for 19 days. Total RNA was isolated from hindlimb gastrocnemius muscle and processed for RNA-seq mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by 2nd strand cDNA synthesis, end repair and A-tailing, adapter ligation, size selection, PCR amplification, and purification.