GSM6759312: 1 µM 5aCdR - rep 4, RNA-seq; Mesocricetus auratus; RNA-Seq
Cells were rinsed with dPBS then  lifted using TryplE (Thermofisher), suspended in complete medium then washed twice by centrifugation /suspension in dPBS then divided into identical aliquots, pelleted and  frozen at -80C until use.  Genomic DNA and total RNA were isolated from cell pellets using DNEasy(Blood and Tissue) and RNEasy mini kits (Qiagen, Mississauga ON),  respectively.  DNA integrity was verified by 0.8% agarose electrophoresis.   RNA integrity was measured using Agilent's 2200 tapestation(Mississauga ON) and all RIN values exceeded 9.7 RRBS libraries were prepared from 100ng genomic DNA using the Premium RRBS kit from Diagenode (catalogue number C02030032).    RNA-seq libraries were prepared from 1µg total RNAusing Illumina's Truseq stranded mRNA kit (20020594 ) and labelled with TruSeq RNA CD Indexes (20019792)