GSM7527124: Budding yeast, Abf1, 20s, rep1; Saccharomyces cerevisiae; OTHER
DNA was extracted with Phenol-Chloroform-Isoamyl alcohol and concentrated using ethanol precipiation. DNA fragments >500bp were removed using 3x vol Agencourt AmPureXP SPRI beads. Libraries were prepared with the NuGen Ovation Ultralow V2 kit using 10 ng DNA as input and 13 cycles of PCR amplification. For post-adapter ligation and post-amplification purifications, 1.8x vol of Agencourt AmPureXP SPRI beads were used.