GSM7867887: HEK293T_99xGly_c8_-DOX; Homo sapiens; RNA-Seq
Cells were rinsed in PBS and lysed in QIAzol (Qiagen). RNA was extracted using a miRNeasy micro cleanup kit (Qiagen). Libraries were prepared from 500 ng of RNA using a KAPA RNA HyperPrep Kit with RiboErase (Roche) and KAPA Unique Dual-Indexed Adapters (Roche) with 12 cycles of PCR.