GSM8124625: GLI1+ cells from hypoxic mouse lungs; Mus musculus; RNA-Seq
Fresh isolated mouse lung samples were flushed with HBSS and then cut into small pieces and filtered two times with 70um and 40um filters. GLI1+ cells are lineage labeled cells with red fluorescent, we first use CD31, CD45 and Epcam to eliminate non-mesenchymal cells. Then PE is used to select RFP+ GLI1+ cell for the single cell analysis. Library sample index sequences were incorporated as the sample index read and sequenced together on a Nextseq2000 sequencer. Raw reads were aligned against the mouse genome (mm10, ensemble assembly 104) and mapped and counted by StarSolo.