16S rRNA-Seq of metagenome
Libraries were constructed via Illumina MiSeq platform (Illumina, San Diego, CA, USA), qualities checked with Agilent 2100 Bioanalyzer System (Agilent Technologies, Palo Alto, CA, USA) using a DNA 7500 chip, and quantified using Quanti-iTTM PicoGreenTM dsDNA Assay kit (Invitrogen)