GSM6979473: RNAseq_Testes_WT_Mcaf2_rep2; Mus musculus; RNA-Seq
Freshly collected tests from Mcaf2ctrl or Mcaf2KO mice were excised, detunicated, and washed with ice-cold 1x phosphate-buffered saline (PBS). Total RNA was purified from Mcaf2ctrl or Mcaf2-/- whole testis using an RNeasy micro kit (Qiagen) according to the manual provided. RNA quality and quantity were checked using, respectively, Bioanalyzer (Agilent) and Qubit (Life Technologies). PolyA/stranded RNA-Seq library was performed using the Illumina TruSeq Stranded mRNA Library Prep kit (Product Number: 20020595) according to the manufacturer's instructions.