RNA-SEQ of Silene colorata Poir.
Plant tissue was ground in liquid nitrogen and stored at -80C before RNA extraction. RNA was extracted from all samples following steps 2-7 from the protocol of Wang et. al.(2012) and continued from step 2 in the RNAeasy plant kit (Qiagen, Inc., Valencia, CA, USA) manual. Quality and concentration of the extracted RNA was measured using NanoDrop (Thermo Fisher Scientific, Inc., Wilmington, DE, USA). RNA-seq library preparation and sequencing of the samples were performed at SciLifeLabs Facility in Stockholm, Sweden (https://www.scilifelab.se/). 2x100bp paired end read libraries were prepared using TruSeq Stranded Total RNA LT Sample Prep Kit - Set A (with Ribo-Zero Plant). (Serial number RS-122-2401.) using a custom protocol on an Agilent Bravo pipetting robot (Agilent, Santa Clara, USA).