DNA sequence of Acinetobacter johnsonii
Using Illumina, a second-generation sequencing platform, qualified DNA samples were constructed with an insertion fragment of ~400bp, PE150 (pin-end) sequencing was performed, and multiple genomic scaffidi were finally assembled. The completed map adopts the sequencing method of Illumina +PacBio (second generation + third generation). Each sample provides no less than 100 PacBio sequencing data and 100Illumina sequencing data of the genome at the same time, ensuring a more complete and accurate assembly. The completed map can avoid the loss of information of small plasmids (< 15kb). A complete genome containing the plasmid is guaranteed.