GSM7979934: AC_213_RA; Semliki Forest virus; OTHER
Total RNA from VLP supernatant extracted. RT-PCR to amplify transgene sequences (amplicons used for library construction). Samples were sequenced with Novogene using their 'Microbial PCR Product Whole Genome Sequencing' strategy. Amplicons underwent fragmentation prior to PCR-free library preparation and sequencing using NovaSeq PE250 technology.