SARS2 viral stock sequence
RNA was reverse transcribed using LunaScript RT Supermix (NEB), and the resulting cDNA was amplified in ~1200bp tiles using Midnight Amplicon Panel v2 primers (IDT) and Q5 High-Fidelity DNA polymerase (NEB). The amplicon tiles were prepared into barcoded and pooled libraries using the SQK-RBK114 rapid barcoding kit (ONT).