GSM8374150: co4x_x_co4x.seed.experiment_3.RNAseq.replicate3; Capsella orientalis; RNA-Seq
Seeds of manually pollinated crossing were taken out of siliques 6 DAP and put into 20 µl of RNA later solution (Sigma-Aldrich). Each sample consisted of 10-15 siliques. RNA was extracted with RNeasy Plant Mini Kit (Qiagen) according to manufacturer's instruction. 100 ng of RNA was used for RNA Seq library preparation with NEBNext Poly(A)mRNA Magnetic Isolation Module (NEB E7490) and NEBNext Ultra RNA LibraryPrep Kit for Illumina (E7530). For each analyzed genotype there were three biological replicates. Libraries were sequenced on HiSeqX in a 150 bp paired-end mode.