16S rRNA gene sequencing from the midguts of Helicoverpa armigera sixth-instar larvae at 24 h post-ecdysis
A total of 20 midguts from each stage were pooled as a replicate and used for metagenomic DNA extraction. The V3 and V4 hypervariable regions of the 16S rRNA gene were amplified with primers 338F and 806R