PacBio sequence reads of the Lodderomyces elongisporus NRRL YB-4239 (ATCC 11503) genome
L. elongisporus strain NRRL YB-4239 (ATCC 11503) was grown in YPD medium. Spheroplasts were created by zymolyase treatment then lysed with SDS. The sample was phenol/chloroform extracted and precipitated with isopropanol. Following RNAse and proteinase K treatment, DNA was sheared with a Megaruptor 3 to an average fragment length of 13 kb. The library was constructed with the SMRTBell Express Template Prep kit 2.0 (Pacific Biosciences) and sequenced on one SMRTcell 8M using a PacBio Sequel IIe with the CCS sequencing mode and a 30 h movie time. CCS and demultiplexing analysis was done using SMRTLink V10.0.