GSM8262763: Liver, AIMER treated mouse, rep3; Mus musculus; RNA-Seq
Each liver punch was transferred to a 2 mL microcentrifuge tube (VWR, 16466-058) containing zirconium oxide beads (Next Advance, ZrOB10). 1 mL of TRIzol was added to each tube, and samples were lysed using Precellys Evolution tissue homogenizer (Bertin, P000062-PEVO0-A). Next, 200 µL of chloroform was added to each tube followed by repeatedly inverting for 30 seconds to ensure thorough mixing, then incubation at room temperature for 10 minutes. Samples were centrifuged at 20,000 × g for 10 minutes at 4°C. After centrifugation, ~400 µL of the top aqueous phase was transferred to a 96-well RNA binding plate and total RNA was again extracted using SV 96 Total RNA Isolation System (Promega, Z3500). mRNA was isolated from total RNA using poly-A isolation and strand-specific libraries were generated (Novagene, USA) 50M reads (2X 150 bp) per sample were sequenced with the Illumina NovaSeq platform