TraDIS sample of pMS7163B, post conjugation library 2
Genomic DNA was fragmented and tagged with an adaptor sequence in a single bead-linked transposome reaction using the Illumina NexteraFlex DNA Prep Kit (Illumina). Following tagmentation, on-bead purification was performed using kit-supplied reagents. An enrichment PCR targeting DNA fragments containing the mini-Tn5-Cm cassette was performed using the custom Tn5-specific enrichment primer 4844 (5'-AATGATACGGCGACCACCGAGATCTACACTA GATCGCaacttcggaataggaactaagg-3' [transposon-specific sequence is in lowercase]) and supplied index 1 primers to allow for multiplex sequencing.