Brucella cytisi ESC1 genome sequencing
The genomic DNA was randomly sheared into short fragments. The obtained fragments were end repaired, A-tailed, and further ligated with Illumina adapter. The fragments with adapters were size selected, PCR amplified, and purified. The library was checked with Qubit and real-time PCR for quantification, and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina platform, according to effective library concentration and data amount required.