GSM8336934: LNAC9; Rattus norvegicus; miRNA-Seq
Total RNA was extracted from prefrontal cortex and nucleus accumbens tissue samples with Trizol, according to manufacter's protocols. Exosomal RNA was extracted from blood serum using the SeraMir kit, using manufacturer's protocols. Small RNA sequencing was performed on six biological replicate samples per treatment group, per region of interest (prefrontal cortex, nucleus accumbens, or serum). 3' and 5' adaptors were ligated to 3' and 5' end of small RNA, respectively. Then the first strand cDNA was synthesized after hybridization with reverse transcription primer. The double-stranded cDNA library was generated through PCR enrichment. After purification and size selection, libraries with insertions between 18~40 bp were ready for sequencing