GSM8375455: SVBN198, PCOS; Homo sapiens; RNA-Seq
RNA extraction will be performed using the Arcturus PicoPure RNA isolation Kit from AppliedBiosystems P3004, P3012 : RNA-Seq libraries were generated from 100 ng of total RNA using Illumina Stranded mRNA Prep, Ligation kit and IDT for Illumina RNA UD Indexes Ligation (Illumina, San Diego, USA) according to manufacturer's instructions. Following polyA selection, mRNAs were fragmented at 94oC for 8 minutes. DNA library were amplified using 13 cycles of PCR. Surplus PCR primers were further removed by two successive purifications using SPRIselect beads (Beckman-Coulter, Villepinte, France).  The final libraries were checked for quality and quantified using Bioanalyzer 2100 system (Agilent technologies, Les Ulis, France).