Illumina MiSeq paired end sequencing of SAMD00796583
Purification of 1st PCR product: 0.7x the amount of PCR solution in AMPure XP (BECKMAN COULTER) was added to the 1st PCR solution provided to purify the PCR product. The concentration of the purified solution was measured using Synergy H1 (Bio Tek) and QuantiFluor dsDNA System (Promega). A library was prepared by 2nd PCR. The concentration of the prepared library was measured using Synergy H1 and QuantiFluor dsDNA System. The quality of the prepared library was checked using a Fragment Analyzer and dsDNA 915 Reagent Kit (Agilent Technologies). Sequencing was performed using the MiSeq system and MiSeq Reagent Kit v3 (Illumina) at 2x300 bp.