GSM8432097: Epididymal white adipose tissue,  NC-RNAi lentivirus knockdown, NC-RNAi-2; Mus musculus; RNA-Seq
The Trizol reagent (Invitrogen) was used to isolate total RNA from the treated cells, followed by reverse transcription utilizing a reverse transcription reagents kit (TaKaRa) to produce a cDNA template. RNA was extracted, sequenced, and analyzed by a custom service provided by Novogene using an Illumina NovaSeq 6000. The bioinformatics analysis was conducted utilizing the OmicStudio tools available at the following website: https://www.omicstudio.cn/tool.