GSM8019212: H14_MACS_scRNA-seq; Homo sapiens; RNA-Seq
Digested tissue samples were subjected to immunomagnetic RBC depletion followed by immunomagnetic CD45 depletion to isolate non-hematopoietic cells (using 67% of the remaining cells) or immunomagnetic CD34 enrichment (using 33% of the remaining cells). Following enrichment, each of the three cell fractions (RBC depleted, CD45 depleted, CD34 enriched) were resuspended at a concentration of 1000 cells / uL in PBS + 0.04% BSA. Cells were pooled and loaded into the 10x Genomics Chip G. We used Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1 and followed the protocol as provided by 10x Genomics.