RNA-Seq of homo sapiens: adult pulmonary arterial endothelial cells
RNA concentration of library was measured using Qubit RNA Assay Kit in Qubit 3.0 to preliminary quantify and then dilute to 1ng/l. Insert size was assessed using the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA). After the insert size met the expectation, the Bio-RAD CFX 96 fluorescence quantitative PCR instrument was used to accurately quantify the library effective concentration (Library effective concentration > 10nm), and the reagent used was Bio-RAD KIT iQ SYBRGRN.