Oxford Nanopore Sequencing of cloned Methanosarcina spherical virus genome candidate (MetSV1782)
The MetSV genome was cloned into pCR-XL2 and transformed into E. coli according to the manufacturer's protocol. Plasmids were isolated from individual clones and sequenced by Oxford Nanopore Technologies (ONT) sequencing using the rapid barcoding kit (SQK-RBK004).