GSM8169907: HER2 CAR Expressed in T-cell (replicate 2); Homo sapiens; RNA-Seq
RNA was isolated using the RNeasy Plus mini kit (Qiagen). Quality control measures were implemented for each sample before library construction to ensure data reliability. Potential RNA degradation and contamination were assessed through agarose gel electrophoresis. Poly-A selection was employed to remove rRNA from the samples. Following the QC procedures, mRNA was enriched from each sample using oligo(dT) beads. Initially, the mRNA underwent random fragmentation by adding a fragmentation buffer. Subsequently, cDNA synthesis was performed using the fragmented mRNA template and random hexamers as primers.Next, the double-stranded cDNA library was completed through a series of steps, including terminal repair, A-ligation, sequencing adaptor ligation, size selection, and PCR enrichment.