GSM8163252: THP1_PIP_EarlyS_0h_r1; Homo sapiens; RNA-Seq
Immediately following the sort, cells were centrifuged, pelleted, and flash frozen in liquid nitrogen and placed at -80ºC. Pellets were processed in groups of 24 using the Zymo RNA Miniprep Kit and quantified using the Invitrogen Quant-it RNA kit. For THP-1 PIP-FUCCI experiments, 100 ng RNA was subjected to poly-A enrichment, cDNA synthesis, and library prep using the Ultra II Directional RNA-Seq Kit and reads were sequenced 2x75bp on an Illumina NextSeq 500.