GSM8001677: KO; Mus musculus; RNA-Seq
Neutrophils were loaded into a microwell chip using the Singleron Matrix® Single Cell Processing System. Barcoding Beads are subsequently collected from the microwell chip, followed by reverse transcription of the mRNA captured by the Barcoding Beads and to obtain cDNA, and PCR amplification. The amplified cDNA is then fragmented and ligated with sequencing adapters. ScRNA-seq was performed using the GEXSCOPER Single-Cell RNA Library Kit (Singleron) following the Singleron GEXSCOPER protocol. The amplified cDNA is then fragmented and ligated with sequencing adapters. ScRNA-seq was performed using the GEXSCOPER Single-Cell RNA Library Kit (Singleron) following the Singleron GEXSCOPER protocol. The concentrations of neutrophils were adjusted to target a recovery of 6,000–10,000 cells per sample. Libraries of each sample were diluted to 4 nM, pooled, and sequenced on NovaSeq 6000 System with 150 bp paired-end reads (Illumina).