mycelium of shaker fermentation C.cicadae-2
Total RNA was extracted from mycelium in shaker and stationary cultures (ammonium sulphate as nitrogen source) by the TRIzol method. The poly-T Oligo-attached magnet was used to enrich mRNA from the total RNA, which was subsequently fragmented in fragmentation buffer. And then a  total amount of 1.5 g mRNA was used to construct cDNA library for each sample by using Illumina's NEBNext UltraTM  RNA Library Preparation Kit. 200-250 bp cDNA fragments were screened and purified using the AMPure XP system (Beckman Coulter, Beverly, USA). The quality of the libraries were then assessed on an Agilent Bioanalyzer 2100 system and sequenced (150 bp paired-end) on an Illumina Novaseq 6000 platform.