RNA-Seq of E. coli : Vector pQE with YbcM Mutant_Replicate2
Aerobic growth in LB medium at 37 degrees celsius, 180 rpm until OD600nm = 0.5. Addition of 0.05 mM IPTG and growth for an additional hour. 5.10^9 cells pelleted and flash-frozen in liquid nitrogen. Total RNA purification (MAXWELL, PROMEGA). The next step was performed by TGML plateforme :  rRNA depletion, cDNA first-strand synthesis and cDNA libraries preparation (Illumina Stranded Total RNA Prep with Ribo-Zero Plus, Illumina). High throughput illumina sequencing with NextSeq-500 sequencer.