Tenascin-C shapes TRAIL control over tumor immunity
Tenascin-C shapes TRAIL control over tumor immunity
Grafted breast tumor tissue was collected as described previously (doi: 10.15252/emmm.202013270). Tumor samples were fresh frozen Total RNA was extracted using RNeasy® Plus Micro Kit (Qiagen) according to the manufacturer's instructions. Libraries were prepared from RNA using SMARTer Stranded Total RNA-Seq Kit, Pico Input Mammalian (Takara) following the manufacturer's instructions. Briefly, random primers were used for first-strand synthesis, and ribosomal cDNA was cleaved by ZapR V.2 in the presence of mammalian R-probes V.2.