GSM7706508: WT_cGAMP_ATAC, rep2; Mus musculus; ATAC-seq
Cell pellets were resuspended in freezing medium containing 90% FBS and 10% DMSO and aliquoted into cryovials for shippping. Frozen cell samples were thawed quickly in a 37 °C water bath and centrifuged at 500 × g for 5 min at 4 °C. The cell pellets were resuspended by gentle pipetting for 3 min in cold lysis buffer containing 10 mM Tris-HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl2, and 0.1% IGEPAL CA-630. The resuspended cell pellets were centrifuged at 500 × g for 5 min at 4 °C to generate crude nuclei. After removing the supernatant, nuclei were subjected to a transposition reaction for 30 min at 37 °C with gentle mixing. Transposed DNA was purified using the QIAGEN MinElute PCR Purification Kit (QIAGEN 28004). Libraries were prepared using the TruePrep DNA Library Prep Kit (Vazyme TD501) according to the manufacturer's instructions. PCR amplification was performed using N5XX and N7XX barcode oligos provided in the TruePrep Index Kit V2 for Illumina (Vazyme TD202). Amplified libraries were cleaned using AMPure XP beads (Beckman Coulter, Inc.), analyzed using an Agilent 2100 Bioanalyzer (Agilent Technologies), and sequenced using NovaSeq 6000 (Illumina).