GSM8375511: O-SARS-3-dpi-#25; Mesocricetus auratus; RNA-Seq
RNAeasy (Qiagen) was used for RNA extraction To prepare the RNA sequencing library, 500 ng of total RNA was utilized. mRNA purification was carried out using the Dynabeads® mRNA DIRECT™ Micro Purification Kit (Thermo Fisher), followed by library preparation using the NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England BioLabs) in accordance with the manufacturer's protocols. The resulting libraries were sequenced on the Illumina NovaSeq 6000 platform, employing the NovaSeq 6000 S1 Reagent Kit (100 cycles) for paired-end runs. On average, each RNA sample yielded approximately 3-5 x 107 reads.