GSM7527543: ChIP-Seq LNCaPabl_MPC309_AR; Homo sapiens; ChIP-Seq
Cells were double crosslinked with formaldehyde and the bifunctional protein crosslinker disuccinimidyl glutarate (DSG) to preserve protein-DNA and protein-protein interactions. A mixture of two antibodies for AR ChIP was used (AR 441 mouse monoclonal, Santa Cruz Biotechnology; Cat#sc-7305 and AR D6F11 rabbit monoclonal, Cell Signaling Technology; Cat #5153) to maximize AR enrichment and minimize epitope masking. Inputs were used for normalization, and additional IgG controls were included to ensure any low occupancy peaks were specific to AR and not the background. Libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit, quantified on Qubit, pooled at equimolar concentrations.