GSM6586188: CD8 TILs, PBS, scRNAseq; Mus musculus; RNA-Seq
Mice were sacrificed the day after the end of the therapy and tumors were excised and tumor infiltrating lymphocytes were enriched using lymphocyte M density gradient protocol. Then MagniSort mouse CD8 T cell enrichment kit (ThermoFisher, 8804-6822-74) was used to enrich for CD8+ TILs from the cell suspension. An equal amount of cell from each biological replicates (n=3) was pooled together and stained using Zombie aqua Fixable viability kit, anti-mouse CD3 PerCP-Cy5.5, and anti-mouse CD8 PE (Biolegend, 100708). Live CD3+CD8+ cells were sorted and used for scRNA-seq. 13,000 cells were loaded on 10x Genomics Chromium system. Library was performed according to the manufacter's instructions (Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (Dual Index) protocol, 10x Genomics).