GSM6979485: Tumor tissue 3612; Homo sapiens; OTHER
HCC samples and benign liver samples were freshly dissected from a patient who underwent liver transplantation. The procurement procedure was approved by the institution review board of University of Pittsburgh. The procedure was compliant with all regulations related to the protocol. The dissected tissues were minced by scalpel and digested with collagenase/protease solution (VitaCyte, 007-1010) until the tissue was fully digested. The digestion time for each preparation was in a range 45-60 min. The digested tissue was removed and immediately cooled with ice-cold Leibovitz's L-15 Medium (Invitrogen, 11415114) supplemented with 10% fetal bovine serum (Sigma, F4135). The single-cell suspension was verified under the microscope. The number of live cells was estimated by trypan blue staining using a hemacytometer. Follow pipelines for 10x Genomic single-cell and unique molecular index (UMI) barcoding, and LOOP UMI distribution and library construction 10X scRNA-seq + LOOP