Strain isolated from cucumber cotyledons infected with powdery mildew (Podosphaera xanthii), from commercial or experimental plantations located in Mlaga (Spain)
The purity of extracted DNA was determined using a NanoDrop spectrophotometer by measuring the A260/A280 and A260/A230 ratios. DNA integrity and quantity were determined using an Agilent 2200 TapeStation and Qubit 3.0 assay, respectively. Oxford Nanopore Technologies (ONT) sequencing libraries were prepared using a PCR-free method of multiplexing samples with the Rapid Barcoding kit (SQK-RBK004, ONT), and sequencing of the barcoded DNA was performed on a single R9.4/FLO-MIN106 ONT flow cell on the MinION (version Mk1B, ONT) for 48h.