GSM6585959: Vector_2; Mus musculus; miRNA-Seq
Small RNA (<200nt) was extracted and purified using miRNeasy Mini Kit (Qiagen) and RNeasy MinElute Cleanup Kit (Qiagen). The small RNA sequence was performed by BGI (Shenzhen, China). 18-30nt RNA was selected by PAGE gel. The 3' adaptor ligation and 5' adaptor ligation was added. The RNA was reverse transcribed and amplified to enrich the cDNA fragments. The DNA fragments were purified by PAGE gel. The double stranded DNA was denatured and circularised to generate single strand circle DNA.