GSM8124394: 35dpt_Stereo-seq; Homo sapiens; Mus musculus; RNA-Seq
Mouse lung tissue was perfused with 2% paraformaldehyde and fixed overnight at 4 °C. The fixed tissue samples were then embedded in Tissue-Teck OCT. Using a Leica CM1950 cryostat, tissue sections with a thickness of 10 μm were obtained and attached to Stereo-seq chips immediately. The spatial transcriptomics data was obtained using the STOmics technology. Briefly, the tissue sections were adhered to the Stereo-seq chip and heated for 3 minutes at 37 °C using a slide warmer. The chip was then incubated in -20 °C methanol for 30 minutes and stained with a nucleic acid dye (Thermo Fisher, Q10212). The stained chip was imaged using a Ti-7 Nikon Eclipse microscope. The cDNA products were released and purified from the Stereo-seq chips after permeabilization for 26 minutes and reverse transcription. Indexed CDNA libraries were constructed following the manufacturer's protocol. Finally, the CDNA libraries were sequenced on an MGI DNBSEQ-Tx sequencer, with 50 bp for read 1 and 100 bp for read 2.