GSM8000491: TELC-D5-scRNA-rep2; Homo sapiens; RNA-Seq
Cells were dissociated into single cells with a 1:1 mixture of 0.5 mM EDTA and TrypLE Express (Gibco). After passing through 70 μM cell strainers, cell suspensions were converted to barcoded scRNA-seq libraries according to the manufacturer's protocol. scRNA-seq libraries were prepared using a DNBelab C Series Single-Cell Library Prep set(MGI,1000021082). Qubit ssDNA Assay kit (Thermo Fisher Scientific, Q10212) was used to quantify the sequencing library concentration.