GSM8018956: P. lunatus + T. urticae replicate 1; Phaseolus lunatus; RNA-Seq
non-infested (control) or infested with T. urticae Phaseolus detached leaves were collected at 24 hpi (hours post infestation) and frozen into liquid N2. Total RNA was extracted using TRIzol reagent (Invitrogen, Boston, MA, USA) following manufacturer´s instructions. Total DNA-free RNA was cleaned using RNeasy Plant Mini Kit purification columns (Qiagen, Hilden, Germany). RNA libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina according to manufacture's intructions.