Pacbio secuencing of C. longiscapa genome whole plant
Genomic DNA equivalent to 50 g was prepared intoSMRTbell templates as specified by the manufacturer (Pacific Biosciences, part no. 100-259-100), using 25 kbp shearing and 18-50 kbp size selection on the Sage SciencesBluePippin system. The SMRTbells were bound to the v2.0 polymerase and sequenced onthe Sequel system using 10 hour movies, and data were collected from a total of 25 SMRTCell 1M