GSM8375205: Runx2-OE SCs-2; Rattus norvegicus; RNA-Seq
The extracted total RNA was subjected to Agilent 4200 TapeStation (Agilent technologies Santa Clara, US) electrophoresis and quality inspection, and then RNA Lean XP Kit (Cat A63987, Beckman Coulter, Inc. Kraemer Boulevard Brea, CA, USA) and RNase Free DNase Set (Cat # 79254) were used, QIAGEN, GmBH, Germany)， Purify Total RNA. Through amplification by SMART-Seq® HT kit HT Kit, cDNA was obtained and subjected to Qubit and Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US) electrophoresis for quality inspection before being used for backup. According to the experimental operation instructions, amplify fragment cDNA, perform end repair, add A at the 3 'end, connect connectors, and enrich to complete the construction of the sequencing sample library. The concentration of constructed library was detected by Qubit® 2.0 Fluorometer, and its size was verified by Agilent 2100.