ATAC-Seq of Saccharomyces cerevisiae BY4741(replicate2)
Yeast cells were harvested and lysed for 10 minutes at 4 C on a rotation mixer. Tissue samples were homogenized into cell suspensions with cold RSB buffer. The cell suspensions were then incubated with lysis buffer for 10 minutes at 4 C on a rotation mixer and filtered with a 40 m cell strainer. The filtered cells were washed once with cold RSB buffer. Approximately 50,000 nuclei were added to the transposition reaction solution for tagmentation. The Tn5-transposed DNA was purified by AMPure DNA magnetic beads and amplified by PCR.