Pool-seq of midge population dervied from location called Chikopa  from Republic of Malawi
Desiccated larvae were brought back to Japan and stored in a desiccator until rehydration prior to use. Rehydrated larvae were reared on milk-agar diet and then developed to imago as described earlier (Watanabe et al. 2002). The imago were collected for the extraction of nucleic acids.