GSM7966928: H660_231_2_5; Homo sapiens; RNA-Seq
Total cellular RNA was isolated from vehicle (DMSO)- or JG231-treated H660 prostate cancer cell lines using the RNeasy Mini Kt (Qiagen). Indexed, stranded mRNA-seq libraries were prepared from total RNA (100 ng) according to standarding protocols for end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification.