metagenome of HS_D600_fl_1
Total DNA and RNA were extracted using DNeasy PowerWater Kits (Qiagen, Hilden, Germany) and RNeasy Plus Kits (Qiagen, Hilden, Germany), respectively, according to the manufacturers protocol. DNA quality was measured using the Qubit dsDNA Assay Kit in a Qubit 2.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA). RNA quality and integrity were measured using a NanoDrop spectrophotometer (Thermo Fisher Scientific, MA, USA) and the RNA Nano 6000 assay kit in conjunction with the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA), respectively. A total of 35 DNA samples (16 from bottom waters, 12 from euphotic layers, and seven from aphotic layers) (Supplementary Figure S1) and three RNA samples from MS were assigned to metagenomic and metatranscriptomic sequencing, respectively, using the NovaSeq 6000 system (Illumina, San Diego, CA, USA), and 150-bp paired-end reads were generated.