LEHBRO 1_4
Prokaryotic DNA was extracted from fecal sample via standard protocol followed by DNA quantification NanoDrop 1000 spectrophotometer and quality assessment through 1% agarose gel electrophoresis. Further, extracted DNA was prepared for sequencing through library preparation as per the Illumina 16S amplicon-sequencing library protocol to amplify the V3 and V4 regions using standard kits. These prepared libraries were then sequenced on the Novaseq 6000 platform (Illumina, San Diego, CA, USA) to yield 250  2 paired-end reads.