Glycine max (UC8, PI 547450) genome assembly of black saddle i-k allele
Library preparation and long read sequencing were  conducted by the University of Illinois Sequencing Center. The HMW DNA was sheared with a Megaruptor 3 (Diagenode.com) to an average fragment length of 13 kb. Sheared DNAs were run on the BluePippin (Sage Science) using a 0.75% agarose cartridge for size selection of fragments > 10kb in length.  Size selected DNAs were converted to a library with the SMRTBell Express Template Prep kit 2.0. Each library was sequenced on 2 SMRTcells on a PacBio Sequel IIe using the CCS sequencing mode and a 30h movie time.