GSM8423583: control, rep 2, scRNA; Homo sapiens; RNA-Seq
Two organoids of each line were collected at day 105 and single cells were obtained according to the protocol of the Papain Dissociation System (Worthington, Lakewood, USA), with centrifugation steps carried out at 4°C. Library preparation was performed with the 10x Genomics kit according to the manufacturer's instructions (Chromium Next GEM Single Cell 3' Reagent Kits v3.1, 10X Genomics, CA, USA).