MCON3
Total RNA was extracted from cells using the Trizol, and ribosomal RNA was removed using the Ribo_Zero kit (Epicentre, Madison, WI, USA). Fragmented RNA were subjected to first strand and second strand cDNA synthesis following by adaptor ligation and enrichment with a low-cycle according to instructions of NEBNext. Ultra RNA Library Prep Kit for Illumina (NEB, USA). The purified library products were evaluated using the Agilent 2200 Tape Station and Qubit2.0(Life Technologies, USA). The libraries were paired-end sequenced at Guangzhou RiboBio Co., Ltd. (Guangzhou, China) using Illumina HiSeq 3000 platform.