GSM6876568: pre-treated, d17, PTX, targeted DNA-Seq; Homo sapiens; OTHER
The genomic DNA was extracted from at least one million cells using the NucleoSpin Tissue kit (Macherey-Nagel). GBCs were enriched from genomic DNA (1.2 ug) by PCR, using PhusionTM High-Fidelity DNA Polymerase and primers designed to capture GBC flanking regions. Amplified DNA was run on a 2% agarose gel and the 280bp band purified using the QIAquick Gel extraction kit (QIAGEN