RNA-Seq: ethylene-treated 4th leaf of tomato plant 1 after 32 hours of treatment
Total RNA after 3, 8, and 32 hours of treatment was extracted using the GeneJET Plant RNA Purification Mini Kit (Thermo Scientific), according to the manufacturers protocol. DNA was removed using the RapidOut DNA Removal Kit (Thermo Scientific). RNA quantity, quality, and integrity were assessed on the Nanodrop (Nanodrop Technology ), via gel electrophoresis, and with the Bioanalyzer (Agilent) respectively. Library preparation and sequencing were performed by Azenta (Genewiz). Sequencing was performed on the Illumina NovaSeq platform (2x150 bp paired-end sequencing) to obtain 20-30 million reads per sample