GSM6976344: MEEC+OP9-Jag1_24h.3; Mus musculus; ATAC-seq
50,000 cells were lysated with cold lysis buffer (10mM Tris-HCl pH 7.4, 10mM MgCl2, 0.1% IGEPAL CA-630) spinning for 15 at 500g (4C), and DNA tagmentated with Nextera DNA Library Prep Kit (Illumina) ATAC-seq libraries were prepared using the NEB Next High-Fidelity 2x PCR Mastermix (New England BioLabs). Libraries were size selected using Agencourt AMPure XP beads from Beckman Coulter.