GSM8528542: RVSMC, H4K5ac_SIANK1; Rattus norvegicus; OTHER
VSMCs were collected, incubated, and bound to concanavalin A-coated magnetic beads. Then, VSMCs were resuspended, incubated with normal IgG, H3K23ac, H3K27ac, or H4K5ac antibodies overnight at 4 °C, and then incubated with secondary antibodies. The samples were incubated with pA/G-Tnp for transposition. After tagmentation, DNA was isolated, amplified, and purified to construct a library. CUT&Tag was performed with a Hyperactive Universal CUT&Tag Assay Kit for Illumina (pA/G-Tnp) (TD903-01, Vazyme Biotech) according to the manufacturer's instructions.