GSM8411367: cE14M3, 12L, snRNAseq; Rattus norvegicus; RNA-Seq
Nuclei were isolated from the dissected frozen Locus coeruleus (LC) of two groups of rats, AAV-GFP and AAV-htauE14, using the 10x Genomics Chromium Nuclei Isolation with RNAse Inhibitor Kit. Library construction was completed using the Chromium Next GEM Single Cell 3' Kit v3.1 (10x Genomics). The libraries were then sequenced (paired-end) on the Illumina NovaSeq 6000. snRNA-seq