GSM8430849: Sox10-Cre-RFP_S1_L001; Mus musculus; RNA-Seq
Freshly collected epithelium of circumvallate/von Ebner's gland (vEG) tissue were washed with sterile Tyrode's solution. Then, the tissues were dissociated with 0.25% trypsin-EDTA. 5% FBS in DMEM/F12 used to stop the reaction and cells were re-suspend in DMEM/F12 containing 10% FBS and 1% BSA. Library was performed according to the manufacturer's intructions (10x Genomics)