single cell RNAseq of TILs from G2-5700
The h4-1BB KI mice were inoculated subcutaneously in the right rear flank region with MC38 tumor cells. Mice were randomized when the mean tumor size reached approximately 216 mm3. The date of randomization was designated as day 0, and hIgG and ATG-101 were dosed at day 0 and day 3. Tumors were collected at day 4. Tumor tissues were cut into small pieces of 24 mm and transferred into the gentleMACS C Tube containing the enzyme mix. Tissues were dissociated at 37 C. Dead cells were removed by Dead Cell Removal Kit. CD45+ immune cells were isolated by CD45 (TIL) MicroBeads according to the manufacture's protocol. Single cell RNAseq library was generated from 10x platform and sequencing was performed by Illumina NovaSeq 6000.