GSM7635852: UMCV19-T; Homo sapiens; RNA-Seq
Tissue RNA and DNA were extracted with Trizol (ThermoFischer/Invitrogen) and stored at -80oC until use. Stranded RNA-seq libraries were constructed using NEBNext poly(A) mRNA isolation kit with the SWIFT RNA Library Kit and the established protocols. The library construction was accomplished with 500 ng of RNA or 4 ng-200 ng of DNA. During library construction, each of the libraries was indexed in order to multiplex for sequencing.