WGS of influenza A virus: RNA extracted from nasal wipe of pig37101_day6
Influenza viral RNA extraction was performed using a custom QIAamp 96 DNA QIAcube treated kit. Amplicons for sequencing libraries were generated through multi-segment RT-PCR, quantified using Quant-iT dsDNA High Sensitivity Assay, and assessed by QIAxcel Advanced System for size confirmation. Sequencing libraries were generated using Nextera XT Sample Prep kit and Nextera XT Index kit v2.