NVSN Surveillance of RSV  [HOUSTON]
RNA was extracted from patient nasal samples and RNA extracts were converted to cDNA. The cDNA was used for library construction using Twist Library Preparation EF 2.0 Kit and Twist Universal Adaptor System (Twist Biosciences). Pooled cDNA libraries were hybridized with biotin-labeled probes from the RSV Panel (Twist Biosciences, Inc) at 70C for 16 hours. The post-capture pool was further PCR amplified for 12-20 cycles and final libraries were sequenced on Illumina NovaSeq 6000 SP flow cell, to generate 2150 bp paired-end reads.