GSM8374116: Input_treat_pileup; Homo sapiens; ChIP-Seq
Cells were harvested and crosslinker with 1% formaldehyde for 10 min at room temperature. After sonication, immunoprecipitation was performed with SOX9 Rabbit Ab (Sigma-Aldrich, AB5535). The immunoprecipitated complex was washed, and DNA was extracted and purified by MinElute PCR Purification Kit (QIAGEN, #28006). The ChIP-Seq library was prepared using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme, #TD501) according to the manufacturer' s instructions. For ChIP-seq, extracted DNA was ligated to specific adaptors followed by deep sequencing in the Illumina Novaseq 6000 using 150bp paired-end.