GSM6503396: Huh7 P3; Homo sapiens; RNA-Seq
Total RNA of each sample was extracted from the honeybee larvae according to the  instruction manual of the TRlzol Reagent (Life technologies, California, USA). RNA  integrity and concentration were checked using an Agilent 2100 Bioanalyzer (Agilent  Technologies, Inc., Santa Clara, CA, USA). The mRNA was isolated by NEBNext Poly  (A) mRNA Magnetic Isolation Module (NEB, E7490). The cDNA library was constructed following the manufacturer's instructions of NEBNext Ultra RNA Library Prep Kit for Illumina（NEB, E7530）and NEBNext Multiplex Oligos for Illumina（NEB,E7500）.