GSM8374134: cg_x_cr-endosperm.BSseq.replicate1; Capsella grandiflora x Capsella rubella; Bisulfite-Seq
Seeds of manually pollinated crossing were taken out of siliques 6 DAP and the endosperm were manually dissected and absorbed by the small part of tissue paper. Samples were stored at -20 °C and then used for isolation of genomic DNA with DNeasy Plant Mini Kit (Qiagen). Bisulfite libraries were prepared with the Accel-NGS Methyl-Seq DNA Library Kit from Illumina (Cat No. 30096, Swift), and the sequencing was performed at Novogene (Hongkong, China) on a NovaSeq 6000 platform in 150-bp paired-end mode