GSM6758114: set1, AagrOXF 4week thalli, H3, rep2; Anthoceros agrestis; ChIP-Seq
Tissues were fixed with 1% paraformaldehyde for 10 minutes. After quenching and tissue lysis, chromatin was sonicated using a E220 focused-ultrasonicator (Covaris). ChIP-seq libraries were prepared using the Ovation® Ultralow Library Systems V2. 1-10 ng of DNA was used as starting material for input and ip samples. Libraries were amplified using 12-15 cycles on the thermocycler. Libraries were validated using the fragment analyzer.