GSM8262775: LSK cells, Control mouse; Mus musculus; ATAC-seq
Cells were lysed in ice-cold lysis buffer (10 mM Tris-HCl, pH 7.4; 10 mM NaCl; 3 mM MgCl2; 0.1% (v/v) Igepal CA-630) for 10 min on ice. The nuclei was washed and then centrifuged at 500 g for 10 min at 4℃. Libraries were constructed using TruePrep DNA Library Prep Kit V2 for Illumina (#TD501, Vazyme) and TruePrep Index Kit V2 for Illumina (#TD202, Vazyme) according to the manufacturer's protocol.