GSM6879702: Blood_miRNA_UTHBC0075; Homo sapiens; miRNA-Seq
RNA was isolated using the 125 miRNAeasy Mini Kit (Qiagen) and total RNA, including miRNA, was bound to a miRNeasy spin column and washed three times before subsequent elution. miRNA libraries were prepared with the QIAseq miRNA library kit (Qiagen) using 5uL of the extracted total RNA sample. Libraries were produced by sequentially ligating adapters to the 3' and 5' ends of miRNAs. This was followed by reverse transcription into cDNA and subsequent ligation of sample indexes and sequencing adapters. The size selection for libraries was performed using QIAseq beads (Qiagen). Library purity was analyzed using the DNA HS1000 tape on a Tapestation 4200 (Agilent) and quantified with Qubit Fluorometer (Thermo Fisher).