GSM8170118: 119 aBCR IL5 J3; Mus musculus; RNA-Seq
Cell pellets were extracted with Nucleospin RNA plus XS kit (Macherey-Nagel) RNA were first enriched with Oligo(dT) beads and then fragmented for 15 minutes at 94 °C. First strand and second strand cDNAs were subsequently synthesized. cDNA fragments were end repaired and adenylated at 3'ends, and universal adapters were ligated to cDNA fragments, followed by index addition and library enrichment by limited-cycle PCR.