GSM7647786: control_siRNA_biological_replicate2; Rattus norvegicus; RNA-Seq
Total RNA was isolated using mirVana RNA isolation kit (Ambion). Subsequently, DNAase treatment and purification was performed and RNA quality was verified in a bioanalyzer before used for RNAseq library preparation. RIN values were: 9-10. Qiaseq Fast Select – rRNA HMR (Cat. No. / ID: 334385) was used for ribodepletion and paired end directional library was prepared using NEB NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760L), and run on Novaseq SP.