GSM8423644: KRT_104(HIV-1_cmp21); Homo sapiens; RNA-Seq
The total RNA was extracted using the Maxwell® RSC simplyRNA Cells Kit (Promega) according to the manufacturer's instructions. RNA integrity was evaluated as RNA integrity number > 6 using the Agilent 2100 Bio-analyzer (Agilent Technologies, Santa Clara, CA, USA). The cDNA libraries were constructed using TruSeq Stranded mRNA Sample Preparation Kit (Illumina, San Diego, CA, USA) according to the manufacturer's instructions.