ITS metabarcoding of grape must sample from winery D ferment 2A timepoint T4 year 2018
Paired-end sequence data (2x300bp) were quality trimmed (Trimmomatic v0.22), adaptor trimmed at the 3 end (cutadapt 1.2.1) and overlapped to form single synthetic reads (FLASH 1.2.11). These synthetic reads were then demuxed according to the inline barcode sequences, before trimming all adaptor, barcode and primer sequences from the 5' and 3' ends.