GSM6757196: CD11c-IL-33KO, RNA-seq 1; Mus musculus; RNA-Seq
Skin biopsies were homogenized and tissue RNA was isolated using the Qiagen RNAeasy plus kit (Qiagen). RNA integrity was measured with an Agilent RNA TapeStation, and concentration measured with the qubit fluorometer (Invitrogen). RNA libraries for samples with RIN values greater than 7 were used for sequencing, and RNA was normalized to 500ng per sample.  Libraries were generated using the Illumina stranded mRNA prep kit (Illumina) and sequenced using an Illumina NextSeq 550 in a single-end 75-base pair (bp) read configuration, at a depth of at least 30 million reads per sample.