GSM8214733: Col-0, Biological Rep 2, Mock; Arabidopsis thaliana; Bisulfite-Seq
~100 whole seedlings per treatment and biological replicate were pooled, flash frozen, and ground under liquid nitrogen. High molecular weight DNA was exctracted with Machery Nagel Nucleospin Plant II kit according to manufacterer's protocols Genomic DNA Sonicated to 100-300 bp fragments and purified with MiniElute PCR Purification Kit (Qiagen, Germany), incubated at 20 ℃ after add End Repair Mix. Then purified and added a single 'A' nucleotide to the 3' ends of the blunt fragments, purified again and add Methylated Adapter to the 5' and 3' ends of each fragment. Then fragments with 300bp-400bp size-range were purified with QIAquick Gel Extraction kit (Qiagen, Germany) and subjected to bisulfite treatment by using Methylation-Gold kit (ZYMO). Followed with PCR and gel purification (350bp-400bp range fragments were selected). Qualified libraries sequenced pair end on the HiSeq X-ten system.