Bacterial WGS of Brucella melitensis isolated from human blood.
Genomic DNA extraction was performed on freshly sub cultured of Brucella melitensis using QIAamp DNA mini kit. Paired-end sequencing libraries were prepared from QC-passed genomic DNA samples using the Illumina TruSeq Nano DNA Library Prep Kit. Approximately 200ng of DNA was fragmented to a mean size of 350bp using Covaris M220. End-repair and adapter ligation were performed to ensure low chimera formation, followed by size selection using AMPureXP beads. Indexed adapters were ligated for hybridization onto a flow cell.