16S rRNA Metagenome: Mouse16 stool
Mouse stool DNA was extracted using Qiagen DNA Stool Mini Kit. The V4 region of the bacterial 16S rDNA was amplified using primers 515 F (barcoded) and 806 R. Following AMPure magnetic purification beads and a Qubit-3 fluorimeter for purification and quantification, equivalent volumes (8 pM) of the amplicons were sequenced on an Illumina MiSeq sequencer (using Miseq reagent kit v3).