GSM8214962: 1 day_Chow_ LSEC_Iso_S2; Mus musculus; RNA-Seq
Mouse livers were perfused and LSEC were separated by gradient centrifugation and then selected with CD146+ coupled magnetic beads. Quality analysis was performed by FACS. RNA was then isolated using the innuPREP RNA Mini Kit 2.0 (845-KS-2040250, Analytik Jena). RNA libraries were prepared for sequencing using standard BGISeq 500 protocols. RNA was then extracted using the innuPREP RNA Mini Kit 2.0 (catalogue number 845-KS-2040250, Analytik Jena). Finally, the RNA libraries were prepared for sequencing according to the standard protocols for the BGISeq 500 platform. RNA libraries were prepared for sequencing using standard BGISeq-500 protocols