PacBio 16S sequencing of mouse fecal sample
DNA was isolated from stool pellets using the QIAamp DNA Stool Mini Kit. Sequencing of a stool pellet from each mouse before and after treatment was performed by PacBio. 16S amplicons were generated and converted to a PacBio library with the SMRTBell Express Template Prep kit 3.0. The library was sequenced on 1 SMRTcell 8M on a PacBio Sequel IIe using the CCS sequencing mode and a 30hs movie time. CCS analysis was done using SMRTLink V11.0 using the following parameters: ccs min-passes 3 min-rq 0.999.