M.verrucosa (5DR)
Whole genome sequencing libraries were prepared with Illumina-compatible NEXTflex Rapid DNA sequencing Bundle. 500 ng of Qubit quantified DNA was sheared using Covaris S220 sonicator to generate specific fragments with size range of 200-300 bp. The fragments were purified using JetSeq cleanup beads. Purified fragments were end-repaired, adenylated and ligated to Illumina multiplex barcode adaptors as per NEXTflex RapidDNA sequencing bundle kit protocol. Adapter-ligated DNA was purified using JetSeq clean up beads. Resultant fragments were amplified for 3cycles of PCR using Illumina-compatible primers provided in the NEXTflex Rapid DNA sequencing Bundle. Final PCR product (sequencing library) was purified with JetSeqcleanup beads followed by library quality control.