GSM8214658: Align-Unloaded-4_S4; Homo sapiens; RNA-Seq
To isolate RNA from HISMCs on PCL scaffolds, each scaffold was minced in 500 μL TRizol (Ambion, 15596018) using sharp scissors and then vortexed for up to 15 minutes until dissolved. RNA was purified from cells lysed in TRIzol using the RNeasy Plus Mini kit (QIAGEN, 74134), with RNase Free DNase Set (QIAGEN, 79254) to remove residual DNA. RNA concentrations were measured by NanoDrop (ND-2000, Thermo Fisher Scientific). Libraries were prepped using TruSeq total RNA sequencing kit (Illumina, 20020596; TruSeq® Stranded Total RNA Library Prep Human/Mouse/Rat) accoridng to manufacturer's protocols