GSM6979491: ATGB24; Mus musculus; RNA-Seq
RNA was extracted from samples of FACS-sorted endothelial cells with PicoPure™ RNA Isolation Kit (#KIT0204, Thermo Fisher Scientific) following manufacturer instructions with minor modifications. RNA was precipitated with 70% ethanol. To avoid genomic DNA contamination a DNAse step was performed using Invitrogen™ PureLink™ DNase Set (#12185010, Invitrogen). RNA quantity and purity were assessed with the Pico Kit Assay on the Agilent 2100 Bioanalyzer System. SMARTseq2 NEBNext