GSM6757797: WT zebrafish SHMO-treated group (bio rep 3); Danio rerio; RNA-Seq
Total RNA was extracted from larvae 5 days old using the Quick RNA miniprep kit (ZymoResearch, Irvine, USA), according to the manufacturer's instruction and checked for purity by NanophotometerTM Pearl-version 1.2 (IMPLEN, Westlake Village, CA), and integrity (RNA Integrity Number > 7) assessed using the RNA 6000 Pico Kit on a Bioanalyzer (Agilent Technologies, Santa Clara, CA). RNA-seq libraries were generated using the TruSeq stranded mRNA ligation kit (Illumina) from 350 ng ofRNA samples, after poly(A) capture and according to manufacturer's instructions.