GSM8403807: WT_Rep3_RNA; Homo sapiens; RNA-Seq
HCT116 cells of the indicated genotype were cultured to 60 to 70% cell density and were washed and scraped gently with ice cold PBS and snap frozen. Cells were resuspended in lysis buffer and mechanically syringed lysed, RNA was isolated by phenol-chloroform extraction. NEB Next ultra II RNAseq kit