GSM8214589: Parasrampuria_mRNA_Seq_shHD-33full_UAC_1; Homo sapiens; RNA-Seq
Total RNA was Trizol extracted 7 days following infection and poly-A was isolated using magnetic bead purification methods. The isolated mRNA was prepared using the cDNA-PCR Barcoding Kit V14 (Oxford Nanopore Technologies) following the manufacturers protocol and sequenced on a PromethION 2 Solo instrument (ONT). RNA-Seq by cDNA-PCR, short read sequencing by Oxford Nanopore P2 Solo methods