GSM6850100: 1083G; Homo sapiens; RNA-Seq
Tumor cells were firstly minced with PBS consisting of 2U/μL RNAase inhibitor. Under a microscope, single cells were selected and aspirated with pipettes. The selected cells were immediately added lysis buffer for single-cell RNA extraction, reverse transcription and cDNA amplification using Single Cell Full Length mRNA-Amplification Kit (Vazyme, N712) per manufacturer's protocol. RNA-seq libraries were constructed from purified cDNA using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme, TD503) according to the manufacturer's instruction. cDNA libraries were then sequenced on an Illumina NextSeq 500.