GSM8428959: EV_Myxo_rep2_k9me3; Mus musculus; ChIP-Seq
AS described in Lee TI, Johnstone SE & Young RA (Nat Proc 2006, 1:729-748). Fixation was performed using 1% paraformaldehyde (PFA). For H3K9me3 ChIP-Seq, chromatin sonication was performed using 20% duty cycle, 140 peak intensity power, and 200 cycles per burst for 10 min. Successively, the pulldown of chromatin was carried out overnight at 4 °C using the specific antibodies: H3K9me3 (CST, catalog no. 13969). Drosophila spike-in chromatin and antibody were added as internal normalization control following manufacturer's instructions (ActiveMotif, catalog no. 53083, 61686). Reverse cross-linking was performed at 65 °C overnight while shaking at 1,200 rpm. Immunoprecipitated DNA was extracted using the ChIP-DNA purification kit (Zymo Research, catalog no. D5205) following the manufacturer's instructions. Libraries were made using the KAPA HTP Biosystems Library Prep kit (Roche, KK8234) using Perkin Elmer Unique Dual Index Barcodes for NextSeq 2000.