GSM8037176: LV10 072, Day 0; Homo sapiens; RNA-Seq
AML samples were FACS-sorted for scRNA-seq on day 0, and CD4IL10 cells were sorted after expansion protocol for scRNA-seq on day 0. Harvested T cells and remaining AML cells and were co-cultured for 24 hours at a 1:1 (E:T) ratio.  CD4IL10 cells and AML cells were sorted and adjusted to a 1:1 ratio to allow equal representation of AML and CD4IL10 cells in scRNA-seq data. This was repeated twice, so that each AML sample was co-cultured with T cells from two different donors. scRNA-seq was completed at Stanford Functional Genomics Facility (SFGF) using the Chromium Single Cell Gene Expression kit (10X Genomics, CA, USA) and Illumina Novaseq (Illumina Inc, CA, USA) sequencer according to manufacturer's protocols. Library was performed according to the manufacturer's instructions (single cell 3' v3 protocol, 10x Genomics).