GSM7832389: CLP_plus_Sita_20_07; Mus musculus; RNA-Seq
Lung lobes were prepared and transferred to an enzyme mix to dissociate cells. CD45-positive cells were bound to CD45 antibody-coupled beads and then discarded. CD45-negative cells were bound to CD31 antibody-coupled magnetic beads for positive cell sorting. After washing, RNA was isolated from cells. PolyA+RNA was purified  from 100 ng total RNA using Poly(A) mRNA Magnetic Isolation module Kit (NEB E7490L, New England Biolabs). The RNA Sequencing library was prepared with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). Directional RNA Library Prep, single reads run 72 Nu with an average of 26.3 M reads per sample