RNA-seq of Caenorhabditis inopinata, L4, Replicate 5
Nematodes in Trizol were subjected to ten freeze-thaw cycles in liquid nitrogen for tissue disruption. RNA was then isolated with the Qiagen RNeasy kit and resuspended in 15 l of RNAse-free water. We used 100 ng of total RNA for mRNA extraction and Illumina library preparation using the Kapa mRNA Hyper prep kit (KK8580). Samples were sequenced on the Illumina HiSeq 4000 platform at the University of Oregon (https://gc3f.uoregon.edu/).