miRNA-seq of apis mellifera: worker hypopharyngeal gland
First of all, ligated the 3 SR and 5 SR Adaptor. Then, reversetranscription synthetic first chain. Last, PCR amplification and Size Selection. PAGEgel was used to electrophoresis fragment screening purposes, rubber cutting recyclingas the pieces get small RNA libraries. At last, PCR products were purified (AMPure XPsystem) and library quality was assessed.