Viral metagenome from Antarctic soil. Sequencing reads.
Ten grams of soil/rhizosphere were resuspended in 35 milliliters of AKC buffer (1%potassium citrate, 10% PBS, and 150 mM magnesium sulfate). Viruses were physically separated from soil by vortexing andshaking. Cells and debris were filtered through a 0.2 m PES filter (Sterivex, Millipore), and viral particles were concentrated using a 100 kDa (Sterivex, Amicon) conical filter. DNase I was used to remove free DNA at 600U by 1g soil. The DNA was extracted using the DNeasy PowerSoil Pro kit (Qiagen). The shotgun genomic libraries were prepared with the Hyper Library construction kit from Kapa Biosystems (Roche) and sequenced by 151 cycles in an Illumina NovaSeq 6000.