GSM6586326: NF3-exo; Homo sapiens; miRNA-Seq
Total RNA was extracted from exosomes using TRIzol reagent (Invitrogen). Approximately 1 µg of total RNA were used to prepare small RNA library according to protocol of TruSeq Small RNA Sample Prep Kits(Illumina, San Diego, USA). And then we performed the single-end sequencing (1x50bp) on an Illumina Hiseq2500 at the LC-BIO (Hangzhou, China) following the vendor's recommended protocol.