GSM6758142: Wild type RD2715, rep2; Hordeum vulgare; RNA-Seq
Inflorescence meristem tissues at Double ridge stage of contrasting cultivars RD2715 and GP were processed as per the manufacturer's protocol for total RNA extraction using SV Total RNA Isolation System, Promega (WI, USA), followed by an on-column DNase treatment. Quality of RNA extracted is confirmed by 1 % agarose gel electrophoresis and visualised in gel doc, and quantity assessed using Agilent nano-drop. NEBNext® Ultra II Directional RNA library Prep Kit was used to prepare high quality libraries, according to manufacturer's protocols and paired end sequencing reads were generated with Ilumina HiSeq-X sequencing platform.