RNA-Seq of hTERT-RPE1/Fucci(CA)2 fractionated according to Fucci expression by FACS, fraction f3
hTERT-RPE1/Fucci(CA)2 cells were sorted into 96-well plates with 100 cells per well using a FACSAria into 8 fractions according to Fucci expression. After reverse transcription, cDNA was amplified by PCR and a cDNA library was prepared using Nextera XT DNA Library Preparation Kit.