GSM7527781: Granulosa cells, CTX, 6h, rep1; Mus musculus; OTHER
Cells were centrifuged for 5 min at 600×g at 4 ℃. Cell activity was detected with LUNA-FL and counted. The cells are bound to Concanavalin A-coated magnetic beads, and the cell membrane is permeabilized by Digitonin. The enzyme pA-Tn5 Transposase precisely binds the DNA sequence near the target protein under the antibody guidance and results in factor-targeted tagmentation. DNA sequence is tagmented, with adapters added at the same time at both ends, which can be enriched by PCR to form the sequencing-ready libraries. After the PCR reaction, libraries were purified with the AMPure beads and library quality was assessed on the Agilent Bioanalyzer 2100 system. (Hyperactive Universal CUT&Tag Assay Kit for Illumina, vazyme).