LVH117 C1: 24hpi replicate 1
The NEBNext Ultra II RNA Library Prep Kit for Illumina was used for library construction. Messenger RNA was purified using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNAs were synthesized using random hexamer primers followed by the synthesis of the respective complementary cDNAs strands. The libraries were finally ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. All libraries were subjected to quantification using the Qubit Fluorometer, and to a new quality control step using the 2100 Bioanalyzer. Sequencing was done with the Illumina NovaSeq platform using a paired-end 150 bp sequencing strategy.