GSM7527501: A673 (HTO1), A4573 (HTO2), SKNMC (HTO3); Homo sapiens; OTHER
500,000 cells were resuspended in PBS + 1% FBS and spun at 300g for 10 minutes at 4oC and then resuspended in 25 uL Biolegend staining buffer (420201, San Diego, CA). 2.5 uL human TruStain FcX (422301, San Diego, CA) was added per sample and incubated at 4oC for 10 minutes. Hash-Tag Antibodies, used to label the  samples  and  minimize  batch  effects,  were  added  at  250  ng/sample  (0.5 uL/sample) and incubated at 4oC for 30 minutes. Cells were washed twice in 1 mL staining buffer and spun at 300g for 5 minutes at 4oC. Samples were pooled 1:1 at 1000 cells/uL and libraries  were  generated  using  the  3'  V3  10X  Genomics  Chromium  Controller following the manufacturer's protocol (CG000183, Pleasanton, CA). Final library quality was assessed using the Tapestation 4200 (Agilent, Santa Clara, CA) and libraries were quantified by Kapa qPCR (Roche).Pooled libraries were then subjected to paired-end sequencing according  to  the  manufacturer's  protocol