GSM8423874: WT-TM_ATAC-seq_rep2; Mus musculus; ATAC-seq
Splenocytes were isolated and specific CD8 T cell subsets were sorted by FACS. ATAC-seq was performed with TruePrep® DNA Library Prep Kit V2 for Illumina® (Vazyme, #TD501-01).  DNA for CUT&Tag-seq was cleavaged Under Target & Tagmentation by H2A.Z antibody (abcam, #ab4174), Pol II phospho S5 (abcam, #ab5131), H3K4me3 (abcam, #ab8580), H3K27me3 (Millipore, #07-449) in Tcells and Genomic DNA was extracted using the Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro (Vazyme, #TD904). The ChIP experiment was conducted using the ULI-NChIP protocol to detect the enrichment of H3K27ac (abcam, #ab4729). ATAC-seq library was constructed by TruePrep® DNA Library Prep Kit V2 for Illumina® (Vazyme, #TD501-01),  following the manufacturer's instructions. CUT&Tag libraries was performed using the Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro (Vazyme, #TD904), following the manufacturer's instructions.   ChIP libraries were prepared with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, #E7645L), following the manufacturer's instructions.