V3-V4 seq of sediment metagenome
DNA was isolated using the ZymoBIOMICS DNA Miniprep Kit (Zymo Research,USA) according to the manufacturer's instructions. Variable regions of the 16S rRNAgene in the V3-V4 region were selected for amplification in the preparation of libraries.Amplification was performed by real-time PCR on a CFX96 Touch instrument (Bio-Rad, USA) using the qPCRmix-HS SYBR reaction mixture (Eurogen, Russia).High-throughput sequencing was performed with a MiSeq system (Illumina, USA)using the MiSeq Reagent Kit v2 (500cycle) (Illumina, USA).