GSM7968236: Kat6b Dec/+ VPA 3331_3 [080923]; Mus musculus; RNA-Seq
For RNA isolation, medium was removed and replaced with 200 µl DNA/RNA Shield (Zymo, R1100) followed by an RNA extraction using a kit (RNeasy micro, Qiagen, 74004) according to the manufacturer's instructions and including the optional DNase I digest. RNA quantity and quality was assessed using a high sensitivity RNA tape (Agilent, 5067-5579 and 4200 Tapestation, Agilent). 100 ng RNA was used to generate RNA libraries using a sequencing library construction kit (TruSeq RNA Library Prep Kit v2, Illumina) according to the manufacturer's instructions.