GSM8075522: ZR_1; Homo sapiens; OTHER
At day 10 cells were collected (60 million cells per sample) for genomic DNA preps with PureLink Genomic DNA Mini Kit (Invitrogen; 16 columns per sample). 200ug gDNA (to maintain representation) was used for PCRs with Ex Taq polymerase (TaKaRa) to prepare sgRNA libraries following Broad Institute's protocol with P5 mix of oligos and barcoded P7 oligos. Libraries (354 nt product) were gel purified with QIAquick Gel Extraction Kit (QIAGEN), followed by MinElute PCR Purification kit (QIAGEN) for buffer exchange.