GSM8169695: A26, EAECBD, Asymptomatic; Mus musculus; RNA-Seq
On D18, brains were flash-frozen in liquid nitrogen and stored in RNAlater at -80°C. RNA isolation, RNA quality evaluation, cDNA library construction, and Illumina RNA sequencing (RNA-Seq) were performed by NovoGene (Sacramento, CA). Reads were paired-end, 150 base pairs at a depth of 40 million reads. Reads were assessed for quality, trimmed, and mapped to the GRCm39 genome (NCBI), and differential expression was analyzed using CLC Genomics Workbench (Qiagen) using default parameters. Differentially expressed genes (DEGs) with a false discovery rate (FDR) ≤ 0.05 were considered significant. The CLC Differential Expression RNA-Seq workflow utilizes a multi-factorial statistical model based on a negative binomial Generalized Linear Model (GLM) to identify statistically significant genes between groups. Reads were paired-end, 150 bp at a depth of 40 million reads.  Reads were assessed for quality, trimmed, mapped to the GRCm39 genome (NCBI), differential expression analysis in CLC Genomics Workbench (Qiagen). Differentially expressed genes (DEGs) with a false discovery rate (FDR) ≤ 0.05 were considered significant.