RNA-Seq of mus muscles: LLC cell line
RNA samples from LLC cells with and without ectopic integrin 3 expression incubated in suspension in 5% charcoal stripped FBS RPMI were submitted to Novogene for RNA-sequencing. RNA was extracted using the RNeasy RNA Purification Kit (Qiagen, 74104), following the manufacturers instructions. A total of 1g of RNA per sample was used to generate RNA-seq libraries using the NEBNext Ultra II RNA Library Prep Kit (NEB, E7770) following the manufacturers protocol. Libraries were sequenced using the Illumina NovaSeq platform, according to the manufacturers protocol.