GSM8527816: colon paracancerous tissue N-glycoRNA; Homo sapiens; ncRNA-Seq
We used TRIzol to extract total RNA from the tissues, and then 20 µg of RNA was taken to enrich for N- and O-glycoRNAs using the optimized SPCgRNA and TnORNA techniques. Subsequently, 20 µL of the enriched glycoRNA was utilized for the construction of sequencing libraries. RNA libraries for small RNA sequencing were prepared using Illumina HiSeq  /Novaseq or MGI2000