GSM8430805: WT B cells #1, Stim; Mus musculus; ATAC-seq
100,000 unstimulated or stimulated B cells were harvested and frozen in culture media containing FBS and 5% DMSO. Cryopreserved cells were sent to Active Motif to perform the ATAC-seq assay. Cells were thawed, permeabilized with 0.1% NP40, 0.1% Tween-20, and 0.01% digitonin detergents and then incubated with Tn5 transposase with adaptors, according to protocols established by Active Motif. Cells were tagmented using the enzyme and buffer provided in the Nextera Library Prep Kit (Illumina). Tagmented DNA was then purified using the MinElute PCR purification kit (Qiagen), amplified with 10 cycles of PCR, and purified using Agencourt AMPure SPRI beads (Beckman Coulter).