GSM8124556: P21; Homo sapiens; RNA-Seq
Total RNA was extracted from tissue samples using Qiagen RNeasy Mini kit following manufacturer's instructions. RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina following manufacturer's instructions. Sequencing libraries were validated using NGS Kit on the Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA), and quantified by using Qubit 4.0 Fluorometer (Invitrogen, Carlsbad, CA).