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total RNA from samples was extracted using QIAamp Viral RNA Kit (Qiagen, Valencia, CA, USA), then subjected to human rRNA depletion by application of Ribo-Zero rRNA Removal Kit (Illumina, San Diego, CA, USA), following the manufacturers instructions. After removing the contaminating DNA via DNase I, cDNA was generated through reverse transcriptase and dNTPs. Nextera XT DNA Library Prep Kit (Illumina) was then applied for the construction of cDNA library.