RNA-seq of Zea Mays: kernel WT treated
Total RNA was isolated using TRI Reagent (Sigma-Aldrich, St. Louis, MO, USA) and purified with the RNA Clean up protocol (Qiagen, Valencia, CA, USA), according to the manufacturer's instructions (Lanubile et al., 2013). The extracted RNA was quantified using a fluorometric assay (Qubit, Thermo Fisher Scientific Inc. Waltham, MA, USA) and the integrity was checked using the 4150 TapeStation System (Agilent, Santa Clara, CA, USA) as well as gel electrophoresis.cDNA libraries (three lines x two conditions x three biological replicates) were constructed following the manufacturer's instructions of the Universal Plus mRNA-Seq kit (Tecan Genomics, Redwood City, CA, USA) and checked with a fluorometric assay (Qubit, Thermo Fisher Scientific Inc.). Libraries were sequenced on paired-end 150 bp mode on NovaSeq 6000 platform (Illumina, San Diego, CA, USA) at IGA-Tech (Udine, Italy)as service provider.