amplicon of ITS2 and AMF (SSU): grassland soil
DNA from bulk soil was extracted with DNeasy PowerSoil Kit (Qiagen). Target sequences were amplified in triplicates with ITS4/ITS7 primers for fungi and NS31/AML2 primers for AMF. Amplicons were cleaned with AMPure XP magnetic beads before and after adding Nexera XT indices. The purified PCR products were quantified with PicoGreen dsDNA Assay and combined to an equimolar pool. Pools were quantified and quality checked and sequenced with Illumina MiSeq.