dental biofilm of periodontally healthy control
Sub-gingival dental biofilm samples were acquired from the gingival cervices in the healthy group) of each quadrant by gently introducing a sterile paper point #35.DNA extraction from the dental biofilm samples in both groups was performed using the PureLink Microbiome DNA Purification Kit.The Illumina MiSeq system was employed for the analysis of 16S rRNA metagenomics using the Illumina MiSeq Reagent Nano Kit v2 (500 cycles).