RNA sequencing of mango peel
mRNA with polyA tail was enriched by Oligo(dT) magnetic beads. Then the RNA was broken into short fragments by fragmentation buffer, and the first cDNA was synthesized with six-base random hexamers based on the fragment RNA template. Then the buffer, dNTPs(dTTP, dATP, dGTP and dCTP) and DNA polymerase I were added to synthesize the double-stranded cDNA, which was then purified by DNA purification magnetic beads. The purified double-stranded cDNA was then end-repaired, A tail was added and a sequencing joint was connected. Then the fragment size was selected by DNA purification magnetic beads, and the final cDNA library was obtained by PCR enrichment.