GSM7867518: PSCI-2; Rattus norvegicus; RNA-Seq
50 mg hippocampal tissue from each mouse was homogenized in 1 ml of Trizol reagent (Cat # 15596026, THERMO) using a glass tissue homogenizer. Phase separation of Trizol homogenate using chloroform, followed by RNA extraction using an RNA analysis kit according to the manufacturer's instructions (Cat # DNF-471, AATI). RNA Intergrity (RIN) was confirmed via Fragment Analyzer 7100 (AATI) and samples with 28S/18S≥0.7 and RIN≥7 were selected for the next experiment. Oligo dT Selection to enrich the mRNA; RNA fragmentation and cDNA synthesis (second-strand cDNA synthesis with dUTP instead of dTTP); End repair, add A and adaptor ligation; PCR; circularization and make DNA Nanoball