GSM6975614: MCF-7_3; Homo sapiens; RNA-Seq
The samples were sent to Zymo Research for Next-Gen RNA sequencing. The samples were prepared for shipment with DNA/RNA ShieldTM (Zymo Research, USA) by mixing 1 million cells with 500 µl of Shield stabilization solution.To remove rRNA, a method described by Bogdanova et al. (2011) was followed with some modifications. RNA-Seq libraries were constructed from 500 ng of total RNA by using the Zymo-Seq RiboFree Total RNA Library Prep Kit (Zymo Research, USA) according to the manufacturer's instructions. RNA sequencing was done on an Illumina NovaSeq (Illumina, USA) to a sequencing depth of at least 30 million read pairs (150 bp paired-end sequencing) per sample.