GSM8074592: RH VEH-1 replicate ChIP; Mus musculus; ChIP-Seq
LK-enriched BM cells were fixed with 1% formaldehyde for 15 min according to the Active Motif ChIP cell fixation protocol. Fixed cell pellets were submitted to Active Motif (Carlsbad, CA) for ChIP-Seq. An automated system (Apollo 342, Wafergen Biosystems/Takara) was utilized to prepare Illumina sequencing libraries (described by Bentley et al., (2008)). The resulting DNA libraries were then quantified and sequenced on Illumina's NovaSeq 6000 following a final PCR amplification step.