Whole genome of Trametes flavida WTFP2
Library Preparation: The ChIP libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (Catalog: E7645S, New England Biolabs). The samples were further purified using 0.9X AMPure XP beads (Catalog: A63881, Beckman Coulter). Furthermore, the DNA was amplified by 12 cycles of PCR with the addition of NEBNext Ultra II Q5 master mix, and NEBNext Multiplex Oligos for Illumina to facilitate multiplexing while sequencing. The amplified products were then purified using 0.9X AMPure XP beads (Catalog: A63881, Beckman Coulter) and the final DNA library was eluted in 15l of 0.1X TE buffer. Authors: Barkha Sharma (Department of Microbiology, Govind Ballabh pant University of Agriculture & Technology, Uttarakhand, India), Lakshmi Tewari (Department of Microbiology, Govind Ballabh pant University of Agriculture & Technology, Uttarakhand, India)