GSM6615042: T1-A50; Mycobacterium tuberculosis; RNA-Seq
Extraction of total RNA by bead-beating lysis of bacteria in Trizol followed by Chlorform extraction and ethanol precipitation. 500ng of total RNA was taken for rRNA depletion using QIAseq FastSelect−5S/16S/23S Kit (Catalog: 335925, invitrogen) according to manufacturer's protocol. Library was prepared using NEBNext Ultra II RNA-Seq kit for illumina.