Pacbio secuencing of C. longiscapa transcripts whole plant
RNA was extracted from 6 tissues, pooled in a single sample and prepared into SMRTbell templates as specified bythe manufacturer (Pacific Biosciences, part no. 100-259-100) using 25 kb shearing and 18-50 kb size selection on the Sage Sciences BluePippin system. SMRTbells were bound to the v2.0 polymerase and sequenced on the Sequel system using 10-hour movies. A total of 2 SMRT Cell 1M were collected as final data for further analyses