Chloroplast-Seq of Castanopsis fabri : growing fresh leaves
After the sample genome DNA is tested as qualified, the DNA is broken by physical method (ultrasonic), and then the broken DNA is purified to build a sequencing library. The steps are as follows: repair the DNA end, add A to the 3 'end, connect the sequencing connector, recover the target segment by agarose gel electrophoresis, conduct PCR amplification on the target segment, and finally build a sequencing library. Before sequencing, quality inspection shall be carried out on the built library. After the quality inspection is qualified, the Illumina NovaSeq6000 platform shall be used for sequencing