CTX_fa_RNAseq of mus musculus: formalin-injected cortex
Samples were with RNA integrity number (RIN) value >8 thus they were accepted for the library preparation. process . RNA-seq libraries were prepared from total RNA (200ng) using the NEBNext Ultra II RNA Sample Preparation Kit for Illumina (New England BioLabs, Ipswich, MA) according to the manufacturer's protocol. Sequencing runs were executed on the Illumina NextSeq500 instrument (Illumina) using single-end 75-cycle sequencing. Raw sequence data (.bcl files) was converted into fastq files and demultiplexed using Illuminas bcl2fastq software.