16SrRNA of mice feces : with or without TGF-beta signaling in TH22
A 16S rRNA gene fragment comprising V3 and V4 hypervariable regions (16S; 5-TACGGRAGGCAGCAG-3 and 5-CTACCNGGGTATCTAAT-3) was amplified using an optimized and standardized 16S-amplicon-library preparation protocol (Metabiote v2.0, GenoScreen, Lille, France). Sequencing was performed using a 250-bp paired-end sequencing protocol on an Illumina MiSeq platform (Illumina) at GenoScreen. Positive (artificial bacteria community comprising 8 different bacteria (ZymoBIOMICS) and negative (sterile water) control were also included