ITS2_Fungal communities spruce rhizosphere from a Picea abies forest in the Czech Republic
Sixteen soil cores (4 cm diameter) were used to sample all material up to the bottom of the soil organic matter at a defined area of 3 x 3 m. Fungal communities were amplified using primers gITS7 and ITS4 (Ihrmark et al., 2012). Amplicons were sequenced on a Illumina Miseq using 2 x 250 bp chemistry.