GSM7780663: eIF4E1_input_rep1; Toxoplasma gondii; RIP-Seq
monolayers were rinsed with PBS, UV-crosslinked 2x 175mJ/cm2 with 254nm.  Monolayers were syringe lysed and parasites were pelleted twice with PBS washes. Parasites were lysed in (50mM Tris pH7.4, 100mM NaCl, 1% NP40, 0.1% SDS, 0.5% sodium deoxycholate) and HA tag was immunoprecipated for 6h after saving an aliquot for size-matched input library was generated according to eCLIP protocol PMID: 27018577 eCLIP