CFIAFB20140150_Salmonella enterica
Brain Heart Infusion broth was inoculated with a single colony of bacteria and incubated for 16-24 h at 35C. Genomic DNA was isolated from 400 L of culture using the Maxwell 16 Cell SEV DNA Purification kit (Promega, Madison, WI). DNA was then quantified using the Quant-iT High-Sensitivity DNA Assay Kit (Life Technologies Inc., Burlington, ON). Sequencing libraries were constructed from 400 ng of gDNA using the Rapid Barcoding kit (Oxford Nanopore Technologies) according to manufacturers instructions.