GSM8263340: BaF3-gp130:IL-6R wt_stimuliert_3; Mus musculus; RNA-Seq
mRNA was isolated with NucleoSpin RNA (Macherey-Nagel, Düren, Germany; cat. no. 740955.250) according to vendor's manual. The library preparation was performed according to the manufacturer's protocol using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD from Lexogen®. Input mount was 100 ng of total RNA. Bead purified libraries were normalized and finally sequenced on the NextSeq2000 system (Illumina Inc. San Diego, USA) with a read setup of SR 1x100bp.