GSM6758107: AagrOXF 4 week thalli, EMseq, rep1; Anthoceros agrestis; Bisulfite-Seq
Genomic DNA was extracted from 100 mg of 4-week-old gametophyte tissue of A. agrestis using Nucleon PhytoPure (cytiva). Sequencing libraries for genome-wide DNA methylation profiles were generated from 200 ng of genomic DNA using NEBNext® Enzymatic Methyl-seq Kit (New England Biolabs). These libraries were sequenced on a Illumina NextSeq 2000 to generate 100 bp paired end reads.