GSM6616245: D0_1_RNA; Mus musculus; RNA-Seq
Cell lysates from each sample were split to generate the RPF and RNA-seq libraries. Total RNA from the cell lysates used for the RNA-seq libraries preparation was extracted using Qiazol. To generate the RPF libraries, the lysates were digested with Mnase. The lysates were run on a polyacrylamide-Urea gel, and fragments 20-40 nucleotides long were purified. The NEBnext library preparation kit for small RNAs was used to generate libraries from the purified RPFs fragments. Illumina RNA-seq libraries were generated from the total RNA extracted from the cell lysates. Libraries were sequenced on a High Output Flow Cell by a NextSeq 500 sequencer (Illumina), utilizing a 51 bp single-end protocol.