GSM8412817: siNC-1; Bombyx mori; RNA-Seq
Following the directions supplied by the manufacturer, RNAiMAX (Thermo Fisher SCIENTIFIC, USA) was used to transfect BmUFBP1 dsRNA or siRNA. For instance, in a 6-well plate, 5 μg of dsRNA was added to a 300 μL volume of serum-free TC100 culture medium for each well, and the mixture was added to an equal volume of RNAiMAX-containing TC100 culture medium, blended thoroughly, and allowed to stand for 15 minutes. The transfection complexes were then added to the BmN cells' supernatant to complete transfection. RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.