GSM8528625: liver_cont1; Mus musculus; RNA-Seq
Liver samples were ground into a powder using liquid nitrogen and incubated with TRIzol. After centrifugation, the upper aqueous layer containing RNA was further purified through extraction with chloroform/isoamyl alcohol (24:1). RNA was then precipitated with isopropyl alcohol and washed twice with 75% ethanol. Finally, the RNA was redissolved in DEPC-treated water. mRNA was enriched using oligo(dT)-attached magnetic beads. The RNA was fragmented, and cDNA synthesis was initiated using random hexamers as primers. The resulting double-stranded cDNA underwent an end-repair reaction, followed by adaptor ligation. Finally, the library products were amplified by PCR and subjected to quality control.