GSM8432368: RNAseq_6A_D14_rep2; Mus musculus; RNA-Seq
Total cell RNA was extracted using TRIzol Reagent (Thermo Fisher Scientific), from which rRNA was depleted using RiboMinus™ Eukaryote Kit v2 (A15020) (Life Technologies Corporation) as per manufacturer's instructions. RNA-seq libraries were prepared in two biological replicates using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs) as per manufacturer's instructions. Libraries were sequenced on Ilumina Novaseq, generating 150bp pair-end reads.