GSM7867619: HCI-011 GDC9545 rep3; Homo sapiens; ATAC-seq
Flash frozen tumors were pulverized to powder by mechanical force (Covaris cryoPREP).  Tumor powder was lysed by addition of TRIzol Reagent (Thermo Fisher Scientific) and homogenized by mechanical disruption (SPEX SamplePrep).  RNA was isolated from homogenized lysates by chloroform extraction and precipitated using the MagMAX-96 Total RNA Isolation Kit (Thermo Fisher Scientific), according to the manufacturer's protocol. The cells were thawed in a 37 degree celsius water bath, pelleted, washed with cold PBS, and tagmented as previously described (Buenrostro et al., 2013), with some modifications based on (Corces et al., 2017). Briefly, cell pellets were resuspended in lysis buffer, pelleted, and tagmented using the enzyme and buffer provided in the Nextera Library Prep Kit (Illumina).