GSM8123860: AP5_lung; Mus musculus; RNA-Seq
Total RNA was extracted from cell samples using Trizol kit. Firstly, the mRNA was enriched and fragmented. Then, cDNA synthesis, terminal repair and connector linking were performed. Polymerase chain reaction (PCR) reaction and product recovery were performed to complete the preparation of the library. Agilent 2100 BioAnalyzer was used to detect library quality. The Illumina novaseq 6000 platform was used to sequence cDNA library.