GSM7746079: GD1870_10 _mg_ IL12ns_male_t1; Mus musculus; RNA-Seq
Total RNA Total RNA was isolated from PBMCs collected at all sampling timepoints following preparation into single cell suspension using theQiagen RNeasy Plus Mini Kit (74136) RNA-seq libraries were prepared usingNew England Biolab's NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490L), Globin & rRNA Depletion Kit (E7750X), Ultra II Directional RNA Library Prep Kitfor Illumina (E7760L), andMultiplex Oligosfor Illumina (E6440L)according to the manufacturer's instructions. Libraries were pooled at equimolar concentrations and sequenced using Illumina's NextSeq 2000 on one P2-100 kit (2004681)and four P3-100 kits (20040559).