GSM7520412: RSM, M[IL-4], rep1; Mus musculus; RNA-Seq
Total RNA was isolated from BMDM using Tripure reagent and chloroform and subsequently purified using spin columns (PureLink RNA Mini, ThermoFisher). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.