GSM8018825: H3K4me3_T1; Homo sapiens; OTHER
Nuclei were isolated with Nuclear extraction buffer [20 mM HEPES-KOH, pH 7.9, 10 mM KCl, 0.5 mM Spermidine (Sigma #05292–1ml-F), 0.1% Triton X-100, 20% Glycerol and 1× protease and phosphatase inhibitor (Thermo Fisher #78442)]. To aid in nuclear capture, isolated nuclei were incubated with activated Concanavalin A beads (Bangs Laboratories #Bp531) for 15 minutes at room temperature followed by incubation with the desired primary antibody (1:100 dilution) overnight. Libraries were prepares using a commercially available CUT&RUN kit (Epicypher #14-1048).