Transcriptomic Signatures of Classical Monocytes Reveal Pro-Inflammatory Modules and Heterogeneity in Polyarticular Juvenile Idiopathic Arthritis
Transcriptomic Signatures of Classical Monocytes Reveal Pro-Inflammatory Modules and Heterogeneity in Polyarticular Juvenile Idiopathic Arthritis
Blood (70 ml) from RA patients and control group were collected, and peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll density and dextran sedimentation. In both studied groups, monocytes were isolated from PBMCs by depletion of non-monocytes cells using an indirect magnetic labeling system. Classical monocytes were indirectly isolated using the CD16 MicroBeads human kit (Miltenyi Biotec®, Germany). Classical monocytes RNA was isolated using the RNeasy Plus Mini (Qiagen®), and RNA integrity number (RIN ≥ 7) was evaluated by the ScreenTape method using Bioanalyser 2100 (Agilent®). RNA library was constructed using the Quant-seq® 30 mRNA-Seq Library Prep Kit (Lexogen®).