GSM8019361: control_BIOT.009_CTR_72hrs_2; Homo sapiens; RNA-Seq
Total RNA from cellular pellet samples was isolated using TRI Reagent (Merck, USA) with RapidOutput DNA removal kit (ThermoFisher Scientific, USA). The quantity and quality of RNA extracts were analyzed by using a Qubit 4 fluorometer (ThermoFisher Scientific, USA) and Fragment Analyzer (Agilent, USA). Complementary DNA (cDNA) libraries were synthesized using the NEBNext® Poly(A) mRNA Magnetic Isolation Module and NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (NEB, UK). RNA sequencing was performed on the Illumina NovaSeq 6000 platform with the NovaSeq S4 300 kit generating 150 bp pair-end reads (Illumina, USA).