GSM6976217: CXCR6KO OT-1 CD8 T cells from vaccinia-OVA infected skin; Mus musculus; RNA-Seq
WT and CXCR6KO OT1 CD8 T cells were isolated from mouse ears 21 days post ear infection with vaccinia-OVA. Mouse ears were incubated with collagenase D for 1 hour and then smashed on a scored plate. Ear suspension was filtered and then cells were stained with CD8 PE. Cells were then CD8 enriched using Miltenyi magnetic enrichment kit. WT and CXCR6KO OT1 CD8 T cells were then fluorescence activated cell sorted for CD8+, CD45 intravenous negative, CD44+, ZombieRed- and congenic marker (WT=CD45.1, CXCR6KO =CD90.1/GFP)