GSM7832487: 3_CORL88_TAS1440_GSI [scRNA-seq]; Homo sapiens; RNA-Seq
Single cells were collected for the COR-L88 cell line by trypsinization. For KP1, tumors were dissociated using the Tumor Dissociation Kit (Miltenyi #130-096-730) and mechanically separated using gentleMACS™ dissociators (Miltenyi #130-093-235) following the manufacturer's protocol. Single-cell libraries were prepared using the 10x Genomics Chromium Next GEM Single Cell 5'v2 (Dual Index) kit, following the standard manufacturer's protocols, with a single capture lane allocated per sample. The target recovery was set at 8000 cells per lane. Cells were partitioned, followed by reverse transcription of mRNA, and library preparation was carried out according to the user guide provided for the Chromium Connect system.