RNA-Seq of PAO1 flow 3
Samples were DNAse treated with Invitrogen DNAse (RNAse free). Library preparation wasperformed using Illuminas Stranded Total RNA Prep Ligation with Ribo-Zero Plus kit and 10bpunique dual indices (UDI). Sequencing was done on a NovaSeq X Plus, producing paired end150bp reads. Demultiplexing, quality control, and adapter trimming was performed with bcl-convert (v4.2.4)