GSM7867776: Granulosa cells, 92_Post-Treatment; Homo sapiens; RNA-Seq
Granulosa cells (5 x 105/mL) were cultured in collagen/fibronectin coated 60 mm culture plates, detached by trypsinization (TrypLE Express, Gibco, Denmark). Total RNA from granulosa cells was isolated using a commercial RNA extraction kit (Zymo Direct-zol Microprep Kit, Zymo Reserch Corporation, USA). The quality and concentration of RNA was determined using a NanoDrop (ND1000, Nanodrop Technologies, USA). RNA purity and integrity were tested using a 5200 Fragment Analyzer System (Agilent, USA) and scored at 10.