GSM7703223: RBM5-Flag RNP-seq rep3; Homo sapiens; OTHER
RNP-seq, IP-seq, 10-22 PCR cycles. The reads from these libraries look as follows:   NNNNabcNNNNxxxxxxxxxxxxxxxxxxxxxx...xxxxxxxxTGAGATCGGAAGAGCGGTTCAG   Where   - NNNN is a four nucleotide degenerate sequence region. The first to these is to ensure high complexity sequence at the beginning, and the second - to serve as a reference for removing PCR duplications.   - abc is a three letter barcode:  - xxxxxxxxxxxxxxxxxxxxxx...xxxxxxxx denotes the RNA fragmnet insert in the read. The insert starts at position 12 (1-based) of the read, and originate from human (HEK293) RNA fragments 28-70 nt in length.   - The TGAGATCGGAAGAGCGGTTCAG sequence is common for all reads and should be immediately downstream of the insert.