GSM8374210: Low-Risk2; Homo sapiens; ncRNA-Seq
Total RNA samples are qualified by agarose gel electrophoresis and quantified using Nanodrop. We use the commercial kit for tRF & tiRNA-seq library preparation, which includes 3ÿ-adapter and 5ÿ-adapter ligation adaptor ligation, cDNA synthesis and library PCR amplification. The prepared tRF & tiRNA-seq libraries are finally quantified using Agilent BioAnalyzer 2100, then sequenced using Illumina NextSeq 500. Sequencing libraries are size-selected for the RNA biotypes to be sequenced using an automated gel cutter. The libraries are qualified and absolutely quantified using Agilent BioAnalyzer 2100 For standard small RNA sequencing on Illumina NextSeq instrument, the sequencing type is 50bp single_x0002_read.