GSM8214597: lymphoid cell line, Input, replicate 2; Mus musculus; ChIP-Seq
Intracellular proteins were crosslinked with DSG (20 minutes, room temperature) and protein-DNA interactions were fixed with 1% formaldehyde (10 minutes, room temperature). Termination of the crosslinking procedure was performed using glycine. For immunoprecipitation, 70 μl Dynabeads Protein G magnetic beads (Invitrogen) were used. Input DNA or DNA enriched through IP was subjected to sequencing library preparation using the NEBNext Ultra II chemistry (New England Biolabs)