GSM8019026: ISO_SON_1; Homo sapiens; RNA-Seq
Total RNA was isolated using Nucleospin RNA Mini kit (Macherey Nagel) following manufacturer's instructions. The NEBNext Ultra II Directional RNA Library Prep Kit for Illumina was used to process the sample(s). The  sample preparation was performed according to the protocol "NEBNext Ultra II Directional RNA Library Prep  Kit for Illumina" (NEB #E7760S/L). Briefly, rRNA was depleted from total RNA using the Qiagen fast select kit  (#334376). After fragmentation of the rRNA reduced RNA, a cDNA synthesis was performed. This was used for  ligation with the sequencing adapters and PCR amplicification of the resulting product. The quality and yield  after sample preparation was measured with the Fragment Analyzer (see appendices). The size of the  resulting products was consistent with the expected size distribution (a broad peak between 300-500 bp).