ND
PCR amplification targeted the V3-V4 region of 16S rDNA of the isolated DNA was done using specific primers. The employed primers were as follows: forward primers - 5-AGAGTTTGATGMTGGCTCAG-3; reverse primers - 5-TTACCGCGGCMGCSGGCAC-3. Amplicons were purified with Ampure beads, and Illumina barcoded adapters were added through 8 additional PCR cycles to construct sequencing libraries. Library purification and quantification were done using Ampure beads and the Qubit dsDNA High Sensitivity assay kit (Thermo Fisher, USA). Sequencing was performed on an Illumina Miseq system(Santiago, USA). Quality control of raw data involved FastQC (Ver. 0.11.9) and MultiQC (Ver. 1.14), followed by adapter trimming and removal of low-quality reads using TrimGalore (Ver 0.6.5).