GSM7081496: L236C_CSP; Mus musculus; RNA-Seq
Fresh tissue was dissected and minced on ice, and subjected to Collagenase & DNase1 treatment in HBSS for 30 mins. Cell slurry was strained and stained with TotalSeqA hashtag antibodies and DAPI as live/dead marker. Cells were FACS-sorted and combined in a multiplexed pooled sample, confirmed to be >95% viable at time of submission. Library was performed according to the manufacter's instructions (single cell 3' v3.1 protocol, 10x Genomics). Primers for TotalSeqA hashtag antibody sequences were used during cDNA amplification. Sequencing performed on NextSeq 2000 sequencing on P3 flow cell.