GSM6926117: DMSO-D3; Homo sapiens; RNA-Seq
RNA isolation: T-24 cells (approximately 1x106 per well) were seeded in a 6 well plate. Next day, cells were treated with either vehicle (DMSO) control or CCT068127 at 1micro molar concentration for 48 hours. Cells were stored with TRI Reagent (ZYMO Research International, Tustin, CA) at -80 freezer. Genomic RNA was extracted from cells using a Direct-zol RNA MiniPrep Plus (ZYMO Research International, Tustin, CA). 500 ng total RNA for each sample was subjected to NEBNext Poly(A) mRNA Magnetic Isolation (E7490) and resulting mRNA libraries were prepared for RNA-seq by NEB Ultra II kit (New England Biolabs) following manufacturer's protocols and sequenced by NovaSeq (Illumina).