GSM6808105: B-813 [arid1a ko Arid1a ChIP-seq]; Mus musculus; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a 60 Sonic Dismembrator (Fisher Scientifi c). Next generation sequencing library preparations were constructed following the manufacturer's protocol. For each sample, ChIP product was used for library preparation. The ChIP product was treated with End Prep Enzyme Mix for end repairing, 5' Phosphorylation and dA-tailing in one reaction, followed by ligation to adaptors with a “T” base overhang. Adaptor-ligated DNA was then recovered using Beads.