GSM8432151: GSC cells treated with ferroptosis inducer RSL3 (3uM) incubated for 24h in hypoxic oxygen conditions (1% O2, 5% CO2)_3; Homo sapiens; RNA-Seq
RNA from snap frozen pellets were isolated with phenol-chloroform method. RNA was precipitated overnight using ice-cold isopraponol at -80C and washed with 70% ethanol. Resuspended in water and frozen in -80C for further analysis. RNA samples (1000 ng) were treated with KAPA RNA HyperPrep Kit with Riboerase to remove ribosomal RNA. To create libraries adapters KAPA_UDI_Indexes were used. Further fragmentation was performed in 94 C for 5 min. PCR amplification was done for 10 cycles, acording to Kapa Biosystems protocol).