WGS of a liquid culture of Cryptotrichosporon argae strain CM19
Yeast cells from a liquid culture were lysed enzymatically and DNA extracted using a NucleoBond HMW DNA kit (Macherey-Nagel #740160). Subsequently, short fragments <10kb were depleted by preferentially precipitating high molecular DNA in size-selection buffer (for 2x: 2.5% w/v PVP 360000, 1.2M NaCl, 20mM Tris-HCl pH 8) at 10000rcf for 30min, following a Nanopore Community protocol based on "DNA clean-up and size selection for long-read sequencing V.4" by Benjamin Schwessinger. The DNA-library was produced using a ONT SQK-LSK112 kit and the appropriate "NEBNext companion module" according to the manufacturers instructions and the raw-data generation was done on a R10.4 flowcell (FLO-MIN112) with a  MinION Mk1B sequencer under sub-optimal temperature control (1-2C above recommended during the sequencing run). Base-calling was done using Guppy v.6.5.7 together with the "ont-guppy-duplex-pipeline 1.0.3". The latter finds and calls "duplex" raw-reads (ssDNA raw-reads are paired by an algorithm that presumably originated from the same dsDNA molecule and called in unison). All other raw-reads (denominated "simplex") are called as well. All raw-reads (duplex and simplex) were eventually called using the Guppy config file "dna_r10.4_e8.1_sup.cfg" (base-calling with the super high accuracy model) and filtered by a minimum qscore of 10.