RNA-Seq of Senecio coronatus: Agnes Mine Ni hyperaccumulator shoot tissue, biorep 1
The library was prepared using the TruSeq RNA sample preparation kit (Illumina Part #15008136), with the standard Illumina low throughput protocol for total RNA provided by the manufacturer with the following minor modifications; SuperScript III Reverse Transcriptase (Life Technologies) was used for the first strand cDNA synthesis and a Zymo Clean and Concentrator-5 kit (Zymo Research) was used instead of the Agencourt AMPure XP DNA clean up beads for cleaning the ds cDNA and end-repaired cDNA.