GSM8019273: FA-1; Homo sapiens; RNA-Seq
Trizol method After the samples were qualified, the library was constructed. The main process was as follows: (1) Eukaryotic mRNA was enriched by magnetic beads with Oligo (dT); (2) The Fragmentation Buffer was added to randomly interrupt the mRNA. (3) The first cDNA strand and the second strand were synthesized and purified from the mRNA. (4) The purified double-stranded cDNA was end repaired, A-tailed, ligated with sequencing adaptor, and then sequenced with AMPure XP beads Segment size selection; (5) cDNA library was obtained by PCR enrichment.