GSM8075265: 14 days in vitro cortical cultures, nonstimulated, replicate2; Mus musculus; RNA-Seq
RNA was extracted with Trizol reagent and phenol-chloroform extraction, and followed by Dnase treatment and column purification with RNeasy micro kit (Qiagen). Librarries were prepared from 200 ng total RNA by using the TruSeq Stranded mRNA Library Kit (Cat# 20020595, Illumina, San Diego, CA, USA) and the TruSeq RNA UD Indexes (Cat# 20022371, Illumina, San Diego, CA, USA). 15 cycles of PCR were performed