Metagenomic sequencing (Oxford Nanopore) of white-tailed deer (Odocoileus virginianus) lung tissue
Genomic DNA was extracted from lung tissue using a Qiagen DNeasy PowerLyzer PowerSoil Kit according to the manufacturers protocol with minor modifications (see associated manuscript). Library preparations were performed using the SQK-LSK 109 ligation sequencing kit following the manufacturers protocol. Genomic sequencing was conducted on a MinION long-read sequencing platform using R9.4.1 flow cells, and multiplexing 3 samples per flow cell.