Whole genome shotgun sequencing gut microbial profiing of domesticated cats: feces
Total DNA was extracted from fecal samples with Zymogen Quick-DNA Fecal/Soil Microbe 96 Mag Bead kit (Zymo Research Corp., USA) using Powerbead Pro (Qiagen, USA) plates with 0.5 mm and 0.1 mm ceramic beads. Extraction controls included water and a characterized homogenized stool. The homogenous stool samples were derived from a mixture of human stool and were used as controls for DNA extraction and library preparation. All samples were quantified with Quant-iT Picogreen dsDNA Assay (Invitrogen, USA). Libraries were prepared with a procedure adapted from the Nextera XT DNA Library Preparation Kit (Illumina, USA). For BoosterShot (Shallow Sequencing, 2 M reads/sample), libraries were sequenced on an Illumina NovaSeq 6000 using single-end 1  100 reads (Illumina, San Diego, CA, USA).