Oxford Nanopore technology reads of Blastomyces helicus
All raw sequencing files were basecalled using  Dorado version 4.2.0 at 400 bps using the super accurate model. Each run was put on a  MinION Mk1B sequencing device and sequenced using MinKNOW software version  23.07.12 from Oxford Nanopore. Runs lasted 72 hours and produced pod5 files except for the first run, which gave us fast5 files. For the first run, the  strain was barcoded and pooled to be placed on a single r10.4 flow cell. We prepared libraries following the Native Barcoding kit 96 protocol (SQK-NBD112.96) from Oxford Nanopores website. We did three more replicate runs using a single r10.4.1 chemistry flow cell to sequence the library  prepared with the Ligation Sequencing Kit V14 (SQK-LSK114) and protocol from Oxford Nanopore.