GSM7527500: P14 CD8 T cells from draining lymph nodes after skin vaccinia-GP33 infection; Mus musculus; RNA-Seq
P14 CD90.1 CD8 T cells were isolated from WT mouse draining lymph nodes of ears 45 days post ear infection with vaccinia-GP33. Draining lymph nodes were pooled from 7 mice. Mouse lymph nodes  were smashed on a 70um filter. Lymph node suspension was filtered and then cells were stained with CD90.1 PE. Cells were then enriched using Miltenyi magnetic enrichment kit. P14 T cells were then fluorescence activated cell sorted for CD90.1+ CD8+, CD45 intravenous negative, CD44+, DAPI-