GSM8423542: iPS_0day; Homo sapiens; RNA-Seq
Undifferentiated cells (0day) were dissociated with Accuatase at 37C for 10min, and differentiated cells (14days) were dissociated with Accuatase at 37C for 30min. Collected cells were washed with PBS containing 0.04% Bovine Serum Albumin (BSA) and resuspended at the appropriate concentration. Libraries were prepared using the Chromium Next GEM Single Cell 3' Kit v3.1(10x Genomics) according to the manufacturer's protocol.