RNAseq of control pulldown of Nuclear fraction RNA of HeLa cells
RNA extraction from the nuclear fraction of HeLa cells followed by pulldown with beads without lipids and recovery of bound RNA with trizol, for sequencing ribosomal RNA was removed. After RNA fragmentation, the first strand cDNA was synthesized, a second strand cDNA synthesis, end repair, A-tailing, adapter ligation, size selection, amplification, and purification