GSM7745734: Male-Ctrl 1; Mus musculus; RNA-Seq
Mice were euthanized by rapid cervical dislocation. Their brains were sliced on a brain matrix (RWD Life Science, Shenzhen, China) in ice-cold dissection buffer (2.6 mM KCl, 1.23 mM NaH2PO4, 26.2 mM NaHCO3, 5 mM kynurenic acid, 212.7 mM sucrose, 10 mM dextrose, 0.5 mM CaCl2, 1 mM MgCl2). The LHb was rapidly dissected out on ice and snap-frozen on dry ice. Total RNA was extracted using the RNAeasy Micro Kit following the manufacturer's protocol (Qiagen, Hilden, Germany). RNA libraries were prepared for sequencing using standard Illumina protocols