GSM6757192: CD11CCRE, scRNAseq; Mus musculus; RNA-Seq
Abdominal skin-resident myeloid cells from control or CD11-IL-33KO mice were sort-purified as Live CD45+ MHC-II+ CD11c+ cells using an BD FACS Aria II sorter. Sort-purified cells were resuspended in PBS supplemented with 10%FBS to achieve a target cell concentration of 700 to 1200 cells per μl. Cell viability was determined by trypan blue exclusion and only samples with >85% viability were processed. Next-generation sequencing libraries were prepared using the 10x Genomics Chromium Single Cell 3' Reagent kit v3 (10X Genomics) per manufacturer's instructions. Libraries were uniquely indexed using the Chromium dual Index Kit, pooled, and sequenced on an Illumina NovaSeq 6000 sequencer in a paired-end, dual indexing run. Sequencing for each library targeted 20,000 mean reads per cell