16S rRNA gene amplicons from nasal bovine swabs collected from dairy calves and cow diagnosed with Bovine Respiratory Disease and healthy pen-mates in New York.
The extracted DNA from know 20 Bacteria strains were used to construct the 16S rRNA gene library (V4 16S rRNA gene) following the protocol from the Schloss lab and Centeno-Martinez et al., 2022.  The 16S rRNA gene amplicons were sequenced using Ilumina MiSeq (2x250, paired-end).