GSM8215058: Retina_Uninfected S7; Mus musculus; RNA-Seq
Retinas were gently peeled away and transferred to ice cold PBS. A Nuclei PURE Prep Nuclei Isolation Kit (Sigma – Cat. # NUC-201) was used and Nuclei were counted in a hemacytometer and used for 10x Genomics RNAseq analysis 8,000 nuclei per retina pool were loaded on a 10X Genomics Next GEM chip and single-nuclei GEMs were generated on a 10X Chromium Controller. Subsequent steps to generate cDNA and sequencing libraries were performed following 10X Genomics' protocol using the Chromium Next GEM Single Cell 3? Reagent Kits v3.1