WGS of Escherichia coli isolated from lake sediments
Multiplexed DNA libraries were prepared using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs) with NEBNext Multiplex Oligos for Illumina (New England Biolabs) according to the manufacturers instructions. Paired-end WGS was performed using the MiSeq platform (Illumina) using v3 reagents (600-cycle) (Illumina) with a read length of 300 bp at the Newfoundland and Labrador Public Health Laboratory (St. Johns, Canada).