GSM8263020: 5 weeks, three biological replicate pool, scRNAseq; Homo sapiens; RNA-Seq
Dissociated single organoid cells were encapsulated into oil droplet that contained barcoded beads, single cell, primers and enzymes by Chromium controller. Barcoded mRNAs from each cell were reverse transcribed and isolated Library was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics).  Chromium Single Cell v2 Reagent Kit and Controller (10X Genomics, Pleasanton, CA, USA)