GSM6759287: UCLA1_hTET1_CDKO1105_hESC_bACEseq; Homo sapiens; OTHER
tion was carried out using EZ DNA Methylation-Direct™ Kit (Zymo Research Cat# D5020).  Eluate was denatured at 95°C and snap cooled by transfer to a PCR tube rack pre-incubated at −80°C.   After thawing, 1.5μL 40mM MES pH 6.0 + 0.1% Tween and 1.5μL 50μM A3A were added to each reaction to a final volume of 15μL. The deamination reactions were incubated at 37°C for 2h, purified with 1.6x homebrew SPRI beads and eluted.  After deamination, the samples were prepared for Illumina sequencing using the Accel Methyl-NGS kit (Swift Biosciences). bACEseq