GSM7082580: 3NNK-EGFP in-vitro ligation-PCR (1ng) library inital; Homo sapiens; OTHER
The positive cell genome was extracted after flow sorting.Genomic DNAs were extracted with a TaKaRa MiniBEST Universal Genomic DNA Extraction Kit Ver. 5.0 (TaKaRa).  High-fidelity KOD-Plus-Neo DNA polymerase was used to amplify the sequences containing the degenerate codons. PCR products were subjected to sequencing library construction by using TruSeq® DNA PCR-Free Sample Preparation Kit (Illumina).