GSM6757776: WT Atg16L1 sample; Mus musculus; RNA-Seq
Single cell suspensions were obtained as previously described in Hoste, E., et al., Epithelial HMGB1 Delays Skin Wound Healing and Drives Tumor Initiation by Priming Neutrophils for NET Formation. Cell Rep, 2019. 29(9): p. 2689-2701 e4. Live cells were sorted on FACS and resuspended in PBS with 0.04% BSA at a final concentration of 1000 cells/ml. Cellular suspensions were loaded on a GemCode Single-Cell Instrument (10x Genomics, Pleasanton) to generate single-cell Gel Bead-in-Emulsion (GEMs). Single-cell RNA-Seq libraries were prepared using GemCode Single-Cell V2 3'Gel Bead and Library kit (10x Genomics) according to the manufacturer's instructions.