GSM7647042: HepG2 CYP3A4 cells, compound 5, day 2; Homo sapiens; RNA-Seq
RNA extraction was performed with the InviTrap®Spin Cell RNA Mini Kit (Invitek Molecular GmbH, Berlin, Germany), according to the manufacturer's instruction. Briefly, the cell pellet was lysed with 350 μL Lysis Solution and treated with β-mercaptoethanol. After DNA removal, 350 μL 70% ethanol were added and the sample applied onto the RNA-RTA Spin Filter. After several washing steps, RNA was eluted with 60 μL of Elution Buffer R and the concentration and purity measured with NanoDrop. Library preparation using the NEBNext© Ultra™ II Directional RNA Library Prep Kit (New England Biolabs, MA, USA),