GSM7867667: SAM24423301_MCF-7, RNP ESR1, sgAA 4 days, rep2; Homo sapiens; RNA-Seq
Total RNA was extracted from duplicates using Qiagen RNeasy kit as per manufacturer's protocol. The concentration of RNA samples was determined using NanoDrop 8000 (Thermo Scientific) and the integrity of RNA was determined by Fragment Analyzer (Advanced Analytical Technologies). Approximately 500 ng of total RNA was used as an input for library preparation using TruSeq RNA Sample Preparation Kit v2 (Illumina). In addition, RNA input of 100 ng was used for library preparation using TruSeq Stranded Total RNA Library Prep Kit (Illumina). The libraries were multiplexed.