GSM7867631: GNE858 rep2; Homo sapiens; ATAC-seq
Cells were detached using Accutase® Cell Detachment Solution (Catalog No. 25-058-CI, Corning), counted and resuspended in growth media containing 5% DMSO. Cells were aliquoted into cryovials, frozen, and shipped to Active Motif (Carlsbad, CA). The cells were thawed in a 37 degree celsius water bath, pelleted, washed with cold PBS, and tagmented as previously described (Buenrostro et al., 2013), with some modifications based on (Corces et al., 2017). Briefly, cell pellets were resuspended in lysis buffer, pelleted, and tagmented using the enzyme and buffer provided in the Nextera Library Prep Kit (Illumina).