RNA-Seq of HIV-1 integrated human monocytic cell line U1: unstimulated
Total RNA was extracted from both unstimulated U1 cells and SAHA-stimulated U1 cells using Trizol reagent. Then, high quality libraries were generated using NEBNext ultra II directional RNA library prep kit following the manufacturer's guidelines. Following library preparation, paired-end sequencing reads with a length of 151 base pairs were generated using the Illumina HiSeq-X sequencing platform