GSM8000066: PHN_2; Mus musculus; RNA-Seq
To isolate single nuclei from PHN of Fos-TRAP2::Ai9 mice treated with panic attacks, we immersed the tissues in ice-cold homogenization buffer containing 250 mM sucrose, 25 mM KCl, 5 mM MgCl2, 10 mM Tris-HCl, 0.1 mM DTT, 1% BSA, 0.1% NP-40, and ribonuclease inhibitor (0.4 U/μl). The tissues were then mechanically homogenized using a glass homogenizer for 10 cycles to obtain a single-nucleus suspension for snRNA-seq. Single-nucleus RNA-seq libraries were prepared using DNBSEQ technology platforms (BGI Genomics, China) and the DNBelab C4 Single-Cell Library Prep Set (MGI Tech, #1000021082).