GSM6927014: PF_NF54_Batch 2, scRNAseq; Plasmodium falciparum; RNA-Seq
Plasmodium falciparum NF54 gametocyte producing cell line were committed to sexual stages (gametocytes) and pooled to 1% gametocytaemia and 50% hematocrit. Gametocyte were membrane fed to Anopheles gambiae adult mosquiteo and dissected at time points; 2, 4, 8, 12 and 20 hours post infection, parasites were then isolated using Micromanupulation based on Anti-Pfs25 antibody conjugated to Alea-488 and Hoechst stain. cDNA library were prepared using SMART-seq2 and ScRNA-seq data were then analyzed using various computational tools. SMART-seq2