RNA-Seq of Candida dubliniensis: CEM106 bcr1/bcr1 mutant
mRNA purified using poly-T oligo-attached magnetic beads. After fragmentation, first strand cDNA was synthesized using random hexamer primers. Second strand cDNA was synthesized using dUTP, instead of dTTP. Directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification.