GSM7832883: 11518X18; Homo sapiens; RNA-Seq
Frozen primary tumor samples embedded in OCT were macrodissected. For RNA extraction and DNase treatment, the Roche High Pure miRNA Isolation Kit was used in accordance with the manufacturer's protocol (5080576001). RNA libraries were prepared using the Illumina TruSeq Stranded Total RNA Kit with Ribo-Zero Gold. Total RNA samples (100-500 ng) were hybridized with Ribo-Zero Gold to substantially deplete cytoplasmic and mitochondrial rRNA from the samples. Stranded RNA sequencing libraries were prepared using the Illumina TruSeq Stranded Total RNA Kit with Ribo-Zero Gold per the manufacturer's instructions (RS-122-2301 and RS-122-2302).