GSM7634844: T1DAL_243767, week 104, DN_NN_CD8; Homo sapiens; ATAC-seq
Bulk ATAC-seq was performed using the method described by Buenrostro et al. 2015. 50,000 cells were lysed to produce a crude nuclei preparation 50,000 cells were lysed to produce a crude nuclei preparation, followed by transposition and PCR amplification of the transposed DNA fragments to add Illumina-compatible indexed adapters. After 1.8x Ampure XP (Beckman Coulter) size selection, concentration of each library was assessed using a 100-1000 bp gate on a Tapestation 4200 (Agilent). Pooled libraries were run with paired-end 59 base sequencing on a NextSeq 2000 with a P3 sequencing kit (Illumina) with a target of 50M reads.