WGS of influenza A virus: RNA extracted from nasal wipe of pig20499_day2
Influenza viral RNA extraction was performed using a custom QIAamp 96 DNA QIAcube treated kit. Amplicons for sequencing libraries were generated through multi-segment RT-PCR, quantified using Quant-iT dsDNA High Sensitivity Assay, and assessed by QIAxcel Advanced System for size confirmation. Sequencing libraries were generated using Nextera XT Sample Prep kit and Nextera XT Index kit v2. Amplicon libraries were purified using 0.8 AMPure XP beads, normalized, then pooled. 6pM of the pooled libraries were sequenced with 5% PhiX added.