AGS D254N
NGS sequencing project started with quality control of the total RNA, where the RNA integrity number (RIN) and concentration are analysed to ensure it is of sufficient integrity and quantity. The library construction of cDNA molecules was carried out using Kapa Stranded mRNA Library Preparation Kit. The generated DNA fragments (DNA library) were sequenced in the lllumina Novaseq platform, using 150bp paired-end sequencing reads.