PacBio HiFi whole genome sequencing of adult male Anastrepha ludens Willacy strain
High molecular weight (HMW) DNA extraction was performed from the using the Qiagen MagAttract HMW DNA Kit. DNA shearing was performed using theDiagenode Megaruptor 2 with the 20 kb fragment protocol. The sheared DNA was prepared for PacBio sequencing using the SMRTBell Express Template kit 2.0. The library was size-selected with beads to remove library molecules less than 3 kb in lengthto generate the final libraries for sequencing. Sequencing was performed on a Sequel II System using Binding Kit v2.0, Sequencing kit v2.0, and SMRT Cell 8M. To target HiFi reads, the library was sequenced using a30hour movie time onone SMRTcell. Raw subreads were converted to HiFi data by processing with CCS to call a single high quality consensus sequence for each molecule, using a 99.5% consensus accuracy cutoff.