GSM6585875: RNAseq_D0_Yaf2_knockout_rep2; Mus musculus; RNA-Seq
Total RNAs were extracted with RaPure Total RNA Micro Kit. In brief, 500 ng of total RNAs were used for RNA-seq library construction. Poly(A)-containing mRNAs were isolated by poly(A) selection beads and further reverse transcribed to cDNAs. cDNAs were ligated with adapters, amplified by PCR and purified with AMPure XP beads to obtain the final sequencing library.RNA sequencing libraries were constructed using a VAHTS Universal V8 RNA-seq Library Prep Kit for Illumina according to the manufacturer's instructions.