GSM7832975: C26 cells, exercise [EXP0222]; Mus musculus; RNA-Seq
To identify transcriptional changes after one bout of exercise, approximately 100 mg of cancer tissue was cut‑off on dry ice, transferred to TRIzol (Invitrogen), homogenized three times at 4,000 rpm for 10 seconds two times using a high throughput tissue homogenizer (Precellys 24, Bertin Technologies). The homogenates were then centrifuged at 14,000 g for 1 minute at 4°C to remove tissue debris. Total RNA was isolated from the Trizol homogenates using the Direct-zol™ RNA MiniPrep Kit (Zymo Research) and RNA integrity was validated using BioAnalyzer (Agilent). cDNA sequencing libraries were generated with the CORALL mRNA-Seq V2 (Lexogen) according to the manufacturers' protocol. All libraries were sequenced on the same run using a NEXTSEQ™1000 device (Illumina) with a read length of 50 bp and a sequencing depth of approximately 20 million reads per sample.