GSM8215264: Mouse kidney tissue_Diabetic (Sample A); Mus musculus; RNA-Seq
Then mice were anesthetized with intraperitoneal injections of sodium pentobarbital (70 mg/kg) and sacrificed by cervical dislocation. The kidneys were taken out. Perform end repair, A-Tailing, adapter ligation, and further amplification steps to attach sequencing adapters to the amplified cDNA. Conduct final library cleanup and quality validation, such as quantification with qPCR and evaluation of library size.