GSM7653702: liver tissue from Ctrl 9; Mus musculus; RNA-Seq
We euthanized animals 24 hrs after administration, and perfused liver samples and blood were harvested. For the obtained liver, a portion of the outer left lateral lobe of the liver was subjected to RNA isolation, and the remaining tissue was subjected to flow cytometry analysis. Total RNA was prepared using Isogen II and purified using an RNeasy Plus Mini Kit with gDNA elimination by an RNase-Free DNase Set, following the manufacturer's protocols. RNA-Seq libraries were prepared with a TruSeq Stranded mRNA Sample Preparation kit. The libraries were sequenced for single-end reading using a NovaSeq 6000 (Illumina).