GSM7646302: 14_D45_Skin; Mus musculus; RNA-Seq
For Lymph nodes, organs were mashed through 70µm cell strainers before lysed with RBC lysis buffer (00-4333-57; eBioscience). For ear and tail skin, separated dorsal and ventral halves were minced and digested at 37 °C for 30 min, with HBSS solution with 1 mg/ml collagenase A (11088785103; Roche) and 40 μg/ml DNase I (10104159001; Roche) before being filtered through 70µm cell strainers. Cells were washed 3 times and kept in PBS supplemented with 2% FBS. OT-1 T cells were then sorted by FACS. RNA libraries were prepared for sequencing using standard Illumina protocols