GSM8374105: SrcGFP (salivary gland) replicate 1, Stage 10-12 embryos, GFP+ cells flow cytometry, sc-RNAseq; Drosophila melanogaster; RNA-Seq
Initial 3 x 1-hour pre-lay collections were discarded, then embryos were collected in 1 and 2-hour intervals on apple juice agar plates, plates were removed and left to age for 5 hours 15 minutes. Aged embryos were dechorinated in 50% bleach for 3 minutes and throughly washed in deionsied water. Embryos were screened for embryonic stage, stage 10-12 were retained and embryos beyond this stage discarded. Embryos were dissociated in a dounce homogonizer (8 strokes of loose pestle, 10 gentle passes through 16G 2-inch needle) and filtered through a 50um filter. Propidium iodide was added to the single cell suspension was sorted for negaitve propidium iodide and positive flourecent tag singal (GFP or YFP). Sorted suspension was kept on ice before library construction. Library was constructed following manufacturers instruction (single cell 3' v3 (SrcGFP_1 and ArmYFP_1) and v3.1 (SrcGFP_2), 10X genomics)