MNGS of homo sapien: cerebrospinal fluid
Each CSF sample was first subjected to bead-beating to lyse organisms, followed by addition (spiking) of T1 (DNA) and MS2 (RNA) bacteriophages as an internal control (IC). Total nucleic acid was then extracted and split into 2 aliquots for construction of separate DNA and RNA libraries. Microbial sequences were enriched by antibody-based removal of methylated host DNA (for DNA libraries) or DNase treatment (for RNA libraries), followed by transposon-based library construction.