GSM8262965: Larval ring gland, pre-NRC starvation, rep2; Drosophila melanogaster; RNA-Seq
Total RNA was extracted using the miRNeasy Micro Kit (QIAGEN, Germany), then stored at ~80 °C. RNA quality, especially DNA contamination, was monitored on 1.5% agarose gels. RNA concentration was measured using NanoDrop 2000 (Thermo Fisher Scientific, USA). RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, USA). Libraries were generated using NEBNext® Ultra™ small RNA Sample Library Prep Kit for Illumina® (NEB, USA) and NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample. The libraries prepared were sequenced on an Illumina Hiseq platform and paired-end reads were generated.