GSM8263074: edited_rep3; Homo sapiens; RNA-Seq
The OmicsCam enveloping the cells was treated with 100 μL of lysis buffer containing 10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% (vol/vol) NP-40, and 1% (vol/vol) BSA, followed by incubation on ice for 5 minutes. Subsequently, 900 μL of washing buffer containing 10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, and 1% (vol/vol) BSA was added. After centrifugation at 1000g for 1 minute, the supernatant was removed, completing the mild lysis of the cells.