GSM6586285: Adult_P_Basal_rep2; Mus musculus; RNA-Seq
The 3rd and 4th mammary glands were isolated, minced and enzymatically digested as described in the materials and methods of the accompanying paper. Cell populations were isolated as described in the materials and methods of the accompanying paper. Cells were lysed in TRIzol LS. RNA was extracted and purified using the Qiagen RNeasy column purification kit. RNA quality was checked with a Bioanalyzer (Agilent), after which polyA+ stranded RNA library preparation was performed using the Illumina TruSeq stranded RNA prep kit. RNA sequencing was performed on a HiSeq2500 (Illumina) system at the NKI Genomics core facility.