GSM6586168: HUVECs, EndMT; Homo sapiens; RNA-Seq
After treatment cells were harvested using trypsin. Cells were diluted in PBS+0.04% BSA to an concentration of 1200 cells / μl. Using the 10x Genomics Chromium Next GEM Single Cell 3' Reagent Kit v3.1 the cells were transferred into oil droplets containing barcoded gel beads. Thereafter, beads are dissolved and reverse transcription into cDNA was done to obtain barcoded full-length cDNA from poly-adenylated mRNA. The obtained cDNA was amplified and used for sequencing.