RNA-Seq of hordeum vulgare seedlings: root zone-specific at 6 h, 24, and 48 h
Morex seedlings (2DAG) were treated with PEG8000 (-0.8 MPa) to simulate water deficit and root samples were collected at 6, 24 and 48 h after stress induction and separated into three distinct root zones: root cap and meristem (CM), elongation zone (EZ) and differentiation zone (DZ) and immediately frozen in liquid nitrogen. In total, 48 samples with three biological replicates for all treatment-root-zone-time point-combinations with a pool of 30 roots for each biological replicate were obtained. Total RNA was extracted with the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturers instructions. The RNA samples were sequenced on a NovaSeq 6000 sequencing platform (Novogene, Cambridge, UK) using a paired-end 150 bp strategy.