GSM7745773: CRISPRi-dSaCas9-KRAB-tiling-B2M-Low-R1; Homo sapiens; OTHER
genomic DNA was harvested using Qiagen's Dneasy Blood and Tissue Kit gRNA libraries were amplified from genomic DNA. All genomic DNA was split across 100 μL PCR reactions (25 cycles at 98C for 10s, 60C for 30s, and 72C for 20s) with Q5 2X Master Mix and up to 1 μg of genomic DNA per reaction. PCRs were pooled together for each sample and purified using double-sided SPRI selection at 0.6x and 1.8x. Libraries were run on a High Sensitivity D1000 tape (Agilent) to confirm amplicon size and quantified using Qubit's dsDNA High Sensitivity assay. Standard gRNA library construction