The complete mitochondrial genome of the sea cucumber
A sequencing library is prepared by simultaneously labeling DNA into short segments of 200-600 base pairs using transposase in a process called labeling, and then connecting the splice to the 3 'and 5' ends of the DNA short segment. Denaturate the prepared sequencing library and load it into the flow pool to generate clusters. During cluster generation, each fragment in the sequencing library undergoes isothermal amplification. The circulation pool is composed of glass containing channels. Each lane is coated with two types of oligonucleotides. One type is a supplement to the 5 'region of the additional motif, while the other type is a supplement to the 3' region of the prepared library's additional motif. Therefore, these oligonucleotides bind to the corresponding DNA regions in the sequencing library.