RNA-seq of Parachordodes pustulosus
Captured worms were preserved in 39.5% ethanol and transported to the laboratory for nucleic acid extraction. Total RNA extracts from specimens were obtained using TRIzol reagent lysis followed by purification with the RNeasy kit (QIAGEN). The sequencing libraries were prepared from total RNA extracts using TruSeq RNA library preparation kit and sequenced with Illumina NextSeq system.