GSM8169664: Jurkat Flp-In T-Rex PURB-eGFP, iCLIP immunoprecipiated for PURB, Mock infected control, rep2; Homo sapiens; OTHER
Cells were washed twice in PBS 1X, resuspended in 3 ml of PBS 1X, crosslinked with two rounds of 0.15 J/cm2 UV light irradiation at 254 nm, washed with PBS 1X and lysed in 1 ml RIPA buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1% Triton X-100, 0.1% SDS, 0.5% wt/vol Na deoxycholate and 0.2 mM AEBSF). Libraries were constructed using iCLIP2 procotol (https://doi.org/10.1016/j.ymeth.2019.10.003)