GB33
The libraries of samples A31-A37 were enriched for a set of 10,207 SNPs according to Ramos Madrigal et al. (2019)6, which corresponds with the GrapeReSeq diversity panel (https://urgi.versailles.inra.fr/Species/Vitis/GrapeReSeq_Illumina_20K). Libraries were captured following the MYbaits protocol version 3.0. Captured libraries were amplified with PCR with 15 L of captured libraries, 2 KAPA HiFi HotStart ReadyMix, 2.5 ul PCR Primer IS5, and IS67. Fourteen cycles of amplification were performed (98C for 2min; 14x 98C for 20 sec, 60C for 30 sec, and 72C for 30 sec; 72C for 5 minutes). Amplified libraries were purified with a Qiagen QIAquick PCR Purification Kit. The libraries concentration was measured using the Qubit dsDNA HS Assay Kit (InvitrogemTM) following the manufacturers protocol, and the captured libraries were also quantified and visualized using the High-Sensitivity D1000 DNA tapes on the TapeStation 4200 (Agilent Technologies), following manufacturers instructions. Finally, captured libraries were pooled based on index compatibility and sample molarity. Captured Libraries were sequenced on an Illumina 2500 HiSeq platform.