GSM8125061: THP-1, Control, rep2; Homo sapiens; RNA-Seq
Total RNA was extracted from LAMs or control cells using TRIzol® Reagent following the manufacturer's instructions (Magen). RNA samples were assessed for purity using the A260/A280 absorbance ratio with a Nanodrop ND-2000 system (Thermo Scientific, USA), and RNA integrity was evaluated using an Agilent Bioanalyzer 4150 system (Agilent Technologies, CA, USA). Only samples meeting quality criteria were selected for library construction. 1 ug of total RNA was used for the construction of sequencing libraries. Paired-end libraries were prepared using a ABclonal mRNA-seq Lib Prep Kit (ABclonal, China) following the manufacturer's instructions.