bulkRNA-seq of homo sapien: GBM stem-like cells PSTAT-treated day2 - SN520: Sample S27
Bulk RNA-sequencing involved RNA isolation from PD-GSCs using miRvana isolation kit (Ambion), which was purified using DNase (Promega M6101). RNA integrity was verified with a BioAnalyzer 2100 (RNA nano chips from Agilent). One microgram of total RNA was used as input for the TruSeq Stranded Total RNA library preparation kit (Illumina). Sequencing was performed on the NextSeq 500 instrument (Illumina) using 2-color chemistry, resulting in a minimum of 25M reads per library with 2 x 75 bp paired-end high output setup.