GSM8338565: TTFs, pMXs-Mef2c, pMXs-TBX5 (MT) Day 7 iCLM reprogramming, replicate 3; Mus musculus; RNA-Seq
Total RNA was extracted from TTFs seven days after retroviral transduction, using TRIzol (Invitrogen) according to the vender's protocol. Illumina RNA-seq was performed by the University of Texas Southwestern Microarray Core Facility. Library construction was performed per manufacturer protocol for mRNA library construction. Illumina RNA-seq was performed by the University of Texas Southwestern Next Generation Sequencing Core Facility.