Whole Genome Sequencing of Genome In A Bottle HG002 DNBSEQ 2X150bp Reads
The sequencing libraries were generated following the standard protocol using PCR amplification by BGI. Briefly, genomic DNA from the NIST GIAB HG002 Reference material was fragmented, and size selected prior to end report and A tailing followed by adapter ligation and size selection. The resulting DNA was PCR amplified prior to sequencing. Sequencing-derived raw image files were processed by DNBSEQ basecalling Software with default parameters and the sequence data was generated as paired-end reads. Reads were quality filtered and aligned to reference using BWA. The resulting bam file were refined following GATK best practices