GSM8403760: 60-M-Pt-H3; Homo sapiens; ChIP-Seq
Tissues were fixed with 1% formaldehyde for 15min. After quenching and cell lysis, chromatin sheared via sonication and incubated with protein A agarose beads conjugated with anti-H3K4me3 and anti-H3K9me3. The DNA was extracted with QIAquick PCR purification kits. ChIP-seq libraries were constructed using TruSeq DNA Sample prep kits and sequenced on an Illumina Novaseq 6000.