ChIP-Seq of E. coli : Vector pQE with YbcM Wildtype_Replicate2
Aerobic growth in LB medium at 37 degrees celsius, 180 rpm until OD600nm = 0.2. Addition of 0.05 mM IPTG and growth for an additional hour. PFA cross-linking, DNA shearing by sonication, immunoprecipitation on FLAG beads, elution by the FLAG peptide, cross-link reversal and DNA purification. DNA libraries prepared with the TruSeq ChIP Sample preparation kit. High throughput Illumina sequencing was performed at the TGML Platform with NextSeq-500 sequencer.