RNA-seq of Cryptococcus bacillisporus CA1873, 37-1
Cells were grown in 50 ml YPD at 37C conducted in duplicate. Cell preparation was spiked in with one-tenth (OD/OD) of S. cerevisiae strain S288C cells grown in YPD at 30C, followed by washing and snap freezing. Total RNA was extracted with TRIzol and then adapted for sequencing using the TagSeq protocol, in which ribosomal RNA was depleted using the RiboZero Yeast reagent. Sequencing was performed at the Broad Institute Genomics Platform, producing 101-base paired reads. TagSeq adapters were removed.