GSM8430359: PolII ChIP, DMSO, Xrn2-444-555Δ, replicate2; Schizosaccharomyces pombe; ChIP-Seq
Cells were disrupted using bead beating, and chromatin was isolated and sheared with a Bioruptor Pico. Immunoprecipitation (IP) was performed using beads coated with antibodies against Rpb1 (8WG16) or Xrn2 (anti-V5) The library was prepared following the ChIP-Seq protocol. Briefly, IP DNA or input DNA was treated with pronase. The samples were then subjected to decrosslinking at 65°C, followed by RNA degradation using an RNase A/T1 mix and purification with the ChIP DNA Clean & Concentrator kit. Purified IP DNA or input DNA was used for library preparation according to the manufacturer's recommendations for the NEBNext Ultra II DNA Library Prep kit with Sample Purification Beads for Illumina.