GSM8375122: PBMC, Post-SR, replicate 1, scRNA-seq; Homo sapiens; RNA-Seq
Prior to taking the initial vein blood sample, volunteers must refrain from smoking and drinking, continue with their regular work schedule, and get plenty of rest (Pre-SR groups). Consequently, they were giving SR boil-free granules for 7 days, 2.5 g each time, three times per day, and after that, their vein blood samples were once again taken as Post-SR groups. During the medication, volunteers must continue to abstain from alcohol and tobacco while taking the medicine, as well as maintain their regular schedule of work and rest. Peripheral blood mononuclear cells (PBMCs) were extracted from the refresh blood after it had been centrifuged utilizing standard density gradient centrifugation Library was performed according to the manufacter's instructions (single cell 3' v3 protocol, 10x Genomics). Briefly, PBMCs were resuspended in the master mix and loaded together with partitioning oil and gel beads into the chip to generate the gel bead-in-emulsion (GEM). The poly-A RNA from the cell lysate contained in every single GEM was retrotranscripted to cDNA, which contains an llumina R1 primer sequence, Unique Molecular Identifier (UMI) and the 10x Barcode. The pooled barcoded cDNA was then cleaned up with Silane DynaBeads, amplified by PCR and the apropiated sized fragments were selected with SPRlselect reagent for subsequent library construction. During the library construction llumina R2 primer sequence, paired-end constructs with P5 and P7 sequences and a sample index were added.