GSM7646311: dentate gyrus, sedentary, rep4 WT; Mus musculus; RNA-Seq
Twelve-month-old p16 WT and p16 KO mice, sedentary, 12 days runner (for RNA-seq) or subjected to a further 7 days of running (protocol 12d RUN + 21d + 7d RUN in Fig 5A), were sacrificed by rapid decapitation­. Bilateral dentate gyrus was dissected under a stereomicroscope according to the procedure described by Hagihara et al. (2009), immediately homogenized in Trizol Reagent (Invitrogen, San Diego, CA, USA), and total RNA extraction was performed as described previously (Farioli-Vecchioli et al., 2012). Extracted RNA was quantified and assessed for purity using a NanoDrop ND-1000 Spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA) and an Agilent 2100 bioanalyzer (Agilent Technologies, Santa Clara CA). RNAs were subsequently employed for Transcriptome sequencing and/or for real-time PCR experiments. Universal Plus mRNA-Seq kit (Tecan Genomics, Redwood City, CA) has been used for library preparation following the manufacturer's instructions (library type: fr-secondstrand)