WGBS of Dicentrarchus labrax: juvenile gills
At the age of 8 months, some fish were transferred to brackish water (BW; osmolality: 475 mOsm kg1) for 24 h and then transferred to dechlorinated tap fresh water (FW; osmolality: 8 mOsm kg1, Na+: 2 mmol L1, Cl: 3.5 mmol L1) for 2 weeks. After the 2-week salinity challenge, five fish per salinity condition were killed using a 100 ppm lethal dose of benzocaine before immediate tissue sampling.The four left gill arches were sampled in five fish/salinity for epigenetic analysis and stored at 80C. Genomic DNA was isolated from a pool of the four sampled gill arches of each juvenile using a Maxwell Blood16 DNA purification kit(Promega)Bisulfite-conversion,library construction and sequencing were performed by Gnome Qubec(Montral, Canada) on an Illumina HiSeq X.