GSM7527853: iPSC-derived thyroid follicular cells (day 12) cultured in thyroid specification medium; Homo sapiens; RNA-Seq
Cells were resuspended in buffer at a concentration of 1000 cells/ul and captured using a 10X chromium instrument (10X Chromium, Pleasanton, CA), following standard Chromium 10x guidelines. Libraries were prepared using a Chromium Single Cell 3' v3 kit (10x Genomics) and loaded on a NextSeq 2000 system (Illumina) aiming for sequencing depth of approximately 89,000 reads/cell