18S amplicon sequencing of Serjania erecta
DNA was extracted using the PowerSoil Pro Kit from QIAGEN. The concentration and purity of the extracted DNA were assessed via electrophoresis in 1.0% (w/v) agarose gels. To amplify the 18S rRNA gene region of fungi, the primers Euk1391F (GTACACACCGCCCGTC) and EukBR (TGACCTTCTGCAGGTTCACCTAC) were used. The amplification products were visualized on 1.5% (w/v) agarose gels. Following quantification, qualification, pooling, and purification, the PCR amplification products were sequenced using the KAPA Library Quantification Kit for Illumina from Roche, on an Illumina MiSeq sequencer, achieving a coverage of 300,000 reads per sample.