GSM8262823: ES-2 shCtl, biological rep1; Homo sapiens; RNA-Seq
RNA was isolated with the Trizol Reagent (Invitrogen) and purified by the Qiagen Column with in column DNase digestion. RNA-seq libraries (non-strand-specific, paired end) were prepared with the NEBNext® Ultra™ RNA Library Prep Kit (Illumina, San Diego, CA) and were sequenced according to the paired-end 150bp protocol using NovaSeq 6000.