GSM8423719: matPΔtetra, Input; Vibrio cholerae; ChIP-Seq
Cells were crosslinked with 1% formaldehyde final concentration. After quenching and cells lysis, samples were sonicated with Covaris. For inmunoprecipitation, Anti-FLAG M2 affinity gel (Sigma) was added to the samples and elution was performed with 3xFLAG. Samples were de-crosslinked and gDNA for Input and IP was extracted with Qiagen PCR Purification Kit. Illumina libraries were prepared using DNA SMART ChIP-Seq Kit (TAKARA)