Transcriptome of MDA-MB-231: adult female breast
RNA Extraction and Purification Total RNA was extracted using the MJzol Animal RNA Extraction Kit (MagBeads) according to the manufacturer's instructions and checked using an Agilent 4200 TapeStation (Agilent Technologies). RIN numbers were used to determine RNA integrity. Eligible total RNA was further purified using the RNAClean XP Kit (Beckman Coulter) and RNase-Free DNase Set (QIAGEN). Purified total RNA was subjected to mRNA isolation, fragmentation, first-strand cDNA synthesis, second-strand cDNA synthesis, end repair, 3'-end plus A, ligation of junctions, and enrichment according to the experimental instructions to complete the library construction of sequencing samples. An Illumina NovaSeq6000 sequencer was used, and the sequencing mode was PE150.