GSM6975626: hos_sib_2_R1_001; Danio rerio; RNA-Seq
For heart-specific RNA-seq, three independent RNA samples for hos mutants and siblings (5 hearts/sample), were isolated and the RNA was extracted using the NucleoSpin RNA XS Kit (Machery-Nagel) according to manufacture's instructions. The libraries for RNA-seq were generated and sequenced on the Illumina HiSeq 2000 by the Genomics Core Facility at University Ulm (Single-end, 50 bp, 10 million reads per sample).