GSM6934336: Female biol rep3; Plasmodium yoelii; RNA-Seq
Total RNA from 2×107sex-sorted gametocytes was extracted using TRIzol. RNA integrity was checked with the Agilent 2100 Bioanalyzer. mRNA was then enriched by Oligo(dT) beads. The enriched mRNA was fragmented into short fragments using fragmentation buffer and reverse transcripted into cDNA with random primers. Second-strand cDNA were synthesized by DNA polymerase Ⅰ, RNase H, dNTP and buffer. The cDNA fragments were purified with the QIAQuick PCR Purification Kit, end-repaired, A tailed, and ligated to Illumina sequencing adapters. The ligation products were size-selected by agarose gel electrophoresis, PCR amplified, and sequenced using Illumina NovaSeq 6000 by Genedenovo Biotechnology Co., Ltd (Guangzhou, China).