GSM6585899: H3K27me3_ChIP_D6_Yaf2_knockout; Mus musculus; ChIP-Seq
107 cells were crosslinked with 1% formaldehyde at RT for 10 min and stopped by adding 0.125 M glycine. Crosslinked cells were lysed in ChIP SDS lysis buffer, then sonicated to achieve chromatin fragments sized of 200-400 bp. ChIPed DNA was eluted using 200 μl freshly prepared elution buffer, reverse-crosslinked and purified using MinElute PCR Purification Kit. ChIP-seq libraries were constructed using VAHTS Universal V3 DNA Library Prep Kit for Illumina according to the manufacturer's instructions.