GSM7081948: COV24H_1; Homo sapiens; RNA-Seq
For RNA isolation, 3 to 6 slices of the hPCLS were pooled and treated with 1 ml of TRIzol reagent (Invitrogen) at room temperature for 30 min.  Total RNAs were then isolated according to the manufacturer's instruction. The RNA samples were checked for quality using Bioanalyzer (Agilent 2100) and were sent to Novogene for library construction per company's protocol.