IT-scC&T-seq of H3K4me3 in a mix of HEK293T and mESCs
Library Strategy: CUT&Tag. Library Selection: CUT&Tag. Approximately 100,000 native HEK293T and 100,000 B6 mESCs were used to extract nuclei, followed by incubation with H3K4me3 antibodies. Secondary antibodies were added, and indexed pA-Tn5 transposase was used for tagmentation. After sorting indexed nuclei into 384-well plates, PCR amplification was performed in two rounds to introduce additional indexes. The resulting libraries were purified, and quality control was performed before sequencing.