WGS of Macaca mulatta: female, blood
Libraries were prepared with either the Nextera DNA (Illumina) or DNA Tagmentation (Illumina) preparation kit. We followed a PCR free protocol for samples containing > 25ng of DNA, while samples containing < 25ng were processed with 12 PCR cycles. Library preparation was followed by clean up and/or size selection using SPRI beads (Beckman Coulter Genomics). After library quantification (Qubit, Life Technologies) and validation (Agilent TapeStation, only for libraries generated using PCR), equimolar amounts per library were pooled. The pools were quantified using the Peqlab KAPA Library Quantification Kit and the Applied Biosystems 7900HT Sequence Detection System. The library pools were sequenced on an Illumina NovaSeq6000 sequencing instrument at the Cologne Centre for Genomics, Germany in paired-end layout yielding 2150 bp reads.