Target-capture of Calanus helgolandicus: from Lenefjord Norwegian Sea
The 179 DNA libraries were prepared following the protocol described in Choquet et al. (2019). Firstly, genomic DNA was extracted from genetically identified specimens using the E.Z.N.A. Insect DNA Kit (Omega Bio-Tek). Secondly, DNA libraries were prepared individually using the NEXTflex Rapid Pre-Capture Combo Kit (Bioo Scientific, Austin, TX, USA) following the recommendations from the producer, with a few adjustments detailed in Choquet et al. (2019). Individually barcoded libraries were then pooled per species and a set of target-capture probes, initially designed for C. finmarchicus (Choquet et al., 2019), was used to perform two successive capture reactions on the species-specific pools following the Mycroarray Mybaits protocol v3 and modifications reported in Choquet et al. (2019). Finally, captured library pools were sequenced in paired-ends on a NextSeq 500 (Illumina), with NextSeq 500/550 2x150 bp mid-output kits v2.5.