GSM7867704: SAM24356481_HCI-003_Ser, GDC-9545, 1 mg/kg, #738; Homo sapiens; RNA-Seq
Flash frozen tumors were pulverized to powder by mechanical force (Covaris cryoPREP).  Tumor powder was lysed by addition of TRIzol Reagent (Thermo Fisher Scientific) and homogenized by mechanical disruption (SPEX SamplePrep).  RNA was isolated from homogenized lysates by chloroform extraction and precipitated using the MagMAX-96 Total RNA Isolation Kit (Thermo Fisher Scientific), according to the manufacturer's protocol. Approximately 500 ng of total RNA was used as an input for library preparation using TruSeq RNA Sample Preparation Kit v2 (Illumina). In addition, RNA input of 100 ng was used for library preparation using TruSeq Stranded Total RNA Library Prep Kit (Illumina). The libraries were multiplexed.