Sequencing of Influenza A/Unknown/Chelyabinsk/206/H7N4
This part of the PB2 segment of A/Unknown/Chelyabinsk/206/H7N4 was identified by capillary sequencing. Four primers (PB2_1F, AGGTCAATTATATTCAATATGGAGAG; PB2_2F,CAAGACGTCATCATGGAGGTCG; PB2_1R,CCTCGATATATACGCTGCTTG; PB2_2R, GCCTCAGGAGCTGATGCATAG) were used to amplify overlapping regions of the incomplete coding sequence of the PB2 segment. Sequencing was performed in 3500 Genetic Analyzer (Applied Biosystems, USA). Sequencing reads of about 700 bp with overlapped at least 100 bp were obtained (phred quality score (Q) 30). The sequenced results were analyzed by the Ugene software.