GSM7706797: IMPRINT5_S437; Bifidobacterium breve; OTHER
Zyppy Plasmid Miniprep Kit A plasmid library containing a stretch of nine randomized nucleotides was used. For NGS library preparation, adapters and unique dual indices based on Illumina TruSeq were added in a two-step amplification process using the KAPA HiFi HotStart Library Amplification Kit (KAPA Biosystems, KK2611), according to manufacturer's protocols. The samples were purified with Agencourt AMPure XP (Beckman Coulter, A63881).