GSM7527852: S2 cells RpS19a input 3; Drosophila melanogaster; RNA-Seq
Extraction using a Promega Reliaprep mini extraction kit. RNA was eluted in 30 μl nuclease-free water, RNA quality was checked using an Agilent Bioanalyzer and RNA concentration was measured using Qubit RNA (broad range) reagents. 1 μg of RNA in 50 μl nuclease-free water was used to prepare sequencing libraries RNA was prepared using the standard TruSeq Illumina protocol, with Oligo-dT beads used to enrich for mRNA