RNA-seq of Homo sapiens: glioblastoma tumor center
Library preparation was performed, starting from 100ng total RNA, using the TruSeq Stranded mRNA Library Kit (Cat# 20020595, Illumina, San Diego, CA, USA) and the TruSeq RNA UD Indexes (Cat# 20022371, Illumina, San Diego, CA, USA). 15 cycles of PCR were performed and the samples were sequenced on an Illumina NextSeq 500 instrument to obtain 38 bp paired-end reads.