Whole genome PacBio sequencing of Xanthomonas arboricola pv. pruni: Strains collected in South America
Genomic samples were fragmented using the Covaris g-TUBE following recommended PacBio procedures. Sequencing libraries were prepared following the PacBio SMRTbell prep kit 3.0 with the SMRTbell barcoded adapter plate 3.0. Pooled libraries underwent the PacBio binding and cleanup steps following the Sequel II binding kit 3.2 and were loaded onto the PacBio Sequel IIe for a 15hr collection period with options enabled to retain subreads and sequencing metrics. Demultiplexing, quality control, and adapter trimming was performed on instrument via Lima.