RNA-Seq of WT-1160 strain
Briefly, total RNA from wild type and erg5 mutant cells(three replicates each) were extracted using TRIzol reagentand then genomic DNA was removed by DNase digestion.Magnetic beads with Oligo (dT) were used to enrich mRNAand fractionate it into short fragments to synthesize cDNA.The purified double-stranded cDNA was then end repaired,3 A tailed, ligated to adapters, and finally, PCR amplificationwas performed to construct each library. An Illumina HiSeqXTen was used for sequencing after each library passed qualityinspection. The clean reads from each sample were retainedfor subsequent analyses.