GSM7520350: HeLa-S283L-atac-rep2; Homo sapiens; ATAC-seq
nuclei were extracted in Nuclei Buffer (10 mM Tris-HCl, pH 7.5, 10 mM NaCl, 3 mM MgCl2, 0.1% Triton X-100) with Roche Complete Protease Inhibitor EDTA-Free. DNA were extracted with Phenol:Chloroform:Isoamyl Alcohol (25:24:1), and precipitated by cold ethanol. 25 µL Tris buffer (10 mM Tris-HCl, pH 7.5) were used to resolve the DNA pellet, and 11.5 µL were used for Nextera library amplification. The library was purified with 1X AMPure XP beads.