GSM7966942: NOR; Mus musculus; RNA-Seq
RNA degradation and contamination was monitored on 1% agarose gels. RNA purity was checked using the NanoPhotometer® spectrophotometer. RNA concentration was measured using Qubit® RNA Assay Kit in Qubit® 2.0 Flurometer. A total amount of 3 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA)following manufacturer's recommendations and index codes were added to attribute sequences to each sample.