iPSC-EC, Tibetan, hypoxia, ind6
The samples in this data set were processed together with an equal number of samples from the CHB population from the 1000 Genomes Project. For each population, we included an equal number of males and females. iPSCs from each subject were differentiated into endothelial cells and then cultured, in parallel, in hypoxia (H) or normoxia (N). RNA extraction was performed in 5 balanced batched of 8 subjects(balanced for population, sex, and treatment) in a Qiagen Qiacube (9001292) using the Rneasy Plus Mini Kit. RNA was assessed for concentration prior to library preparation. 500ng of each sample was used for library prep using Illumina TruSeq2 RNA-seq library prep kit and set A indexes (RS-122-2001). All libraries were assessed via bioanalyzer to be of sufficient quality, concentration, and fragment size for further processing. The corresponding RNAseq data for the CHB samples are publicly available at the GEO repository under accession number GSE271456.