GSM7648583: WT_DOX1; Fusarium fujikuroi; RNA-Seq
Isolation of fungal RNA was performed with lyophilized and ground mycelia from cultivation on solid CM or under liquid-standard conditions (ICI supplemented with 6  glutamine) for 3-4 days. Total RNA was extracted from samples, using the RNA reagent TRIzol (Thermo Fisher Scientific) as described in the manufacturer's manual. Library preparation and sequencing of the samples was performed by the Vienna BioCenter Core facilities (Vienna, Austria).Library prep and sequencing was performed using poly-A enrichment kit (NEB) and Nextera Library prep kit.