Amplicon Sequencing of Multiallelic gene edited Magnesium chelatase Subunit I gene from bahiagrass, spCas9 was used to mediate the mutagenesis
Amplicons of 586 bp were purified. Complete amplicon sequencing was performed at Massachusetts General Hospital (Cambridge, MA) by the CCIB DNA Core Facility. Adapters with unique barcodes were ligated onto each sample during library construction. Libraries were pooled in equimolar concentrations for multiplexed sequencing on the Illumina MiSeq platform (Illumina, San Diego, CA) with 2x150 run parameters.