16s rRNA seq of mus musculus: feces
Bacterial genomic DNA was extracted from fecal samples using the Qiagen PowerSoil Kit. PCR amplification, run in triplicate, of the V4 region of the 16S rRNA gene was completed using individually barcoded universal primers. Samples were sequenced using the Illumina MiSeq platform and 2 x 250bp reagent kit for paired-end sequencing at Laragen, Inc.