WGS and annotation of F. glaucescens
Leaf material was collected from plants clonally propagated and maintained in the greenhouse.  High molecular weight (HMW) genomic DNA was extracted using Nanobind Plant Nuclei Big DNA Kit (Circulomics, PacBio, USA). All samples passed screening QC criteria. For PacBio Sequel sequencing, 8 ug of input genomic DNA was used for HiFi library preparation. For gDNA where the size range was less than 17kb, we used the Femto Pulse System (Agilent) to determine the actual size distribution. The library insert sizes were in the optimal size range, we sheared gDNA was sheared with the Megaruptor 3 (Diagenode) and purified using AMPure PB magnetic beads (Pacific Biosciences) if the apparent size was greater than 40kb. Total 10uL library was prepared using PacBio SMRTbell Experss Template Perp Kit 2.0. SMRTbell templates were annealed using Sequel II Bind Kit 2.2 and Int Ctrl 1.0 The Sequel II Sequencing Kit 2.0 and SMRT cells 8M Tray was used for sequencing. SMRT cells (Pacific Biosciences) using 30hr movies were captured for each SMRT cell using the PacBio Sequel (Pacific Biosciences) sequencing platform by Macrogen(Seoul, Korea). The subsequent steps are based on the PacBio Sample Net-Shared Protocol, which is available at https://www.pacb.com/