GSM8528632: WT_CUT&Tag_2; Mus musculus; OTHER
The Phf23+/+ and  Phf23-/- neurospheres were dissociated enzymatically into single cells, Use NovoNGS CUT&Tag3.0 High Sensitivity Kit. The single cells were treated with ConA beads, incubated with H3K27ac antibody (1:50, rabbit, abcam, ab4729) overnight at 4°C, incubated with secondary antibody for 1 hour at room temperature, and incubated with pAG-Tn5 for 1 hour. Fragment DNA at 37°C for 1 hour and stop with 10% SDS at 55°C for 10 min. Extract DNA fragments and amplify libraries. The libraries were quality-checked on the Agilent5400.