WGBS rep2 of Bc2X
Plant samples used for WGBS were consistent with those for RNA-seq described above. The leaf tissues from two biological replicates were pooled for genomic DNA extraction using a CTAB-based protocol[53]. Quantification were measured by an Agilent 2100 spectrophotometer (Agilent Technologies, Palo Alto, CA, USA). Bisulfite treatment (EZ DNA Methylation-Gold KIT, Zymo, D05005), library construction (TruSeq DNA Methylation Kit, illumina, EGMK91396), and sequencing (iIIumina Novaseq6000) were performed at Oebiotech Company (Shanghai, China).