Whole genome sequencing of Colaptes auratus
DNA was extracted using a Gentra Puregene Tissue kit (Qiagen, California, USA) following the manufacturer's protocol. Cornell Weill Medical College genomics core facility prepared and sequenced three libraries: one 180bp fragment library, one 3kb mate-pair library, and one 8kb mate-pair library. These libraries were sequenced across three lanes of an Illumina HiSeq2500 using the rapid run mode. The mate-pair libraries were multiplexed on a single lane, while the fragment library was run across two lanes.