RNA-Seq of HIV-1 integrated human monocytic cell line U1: unstimulated
Total RNA was extracted from both unstimulated U1 cells and SAHA-stimulated U1 cells using Trizol reagent. The quality and quantity of the extracted RNA were checked by Tapestation 2200 (Agilent) and Qubit 4.0 fluorometer (Thermofisher) respectively. Then, high quality libraries were generated using NEBNext ultra II directional RNA library prep kit following the manufacturer's guidelines. Following library preparation, paired-end sequencing reads with a length of 151 base pairs were generated using the Illumina HiSeq-X sequencing platform