GSM7968199: Mtb infected Zeb2 fl/fl rep1; Mus musculus; RNA-Seq
Qiagen RNeasy micro kit Total RNA integrity was determined using Agilent Bioanalyzer or 4200 Tapestation. Library preparation was performed with 10ng of total RNA with a Bioanalyzer RIN score greater than 8.0. ds-cDNA was prepared using the SMARTer Ultra Low RNA kit for Illumina Sequencing (Takara- Clontech). Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases.