Metatranscriptomic sequencing
Total RNA was extracted using the RNA PowerSoil Total RNA Isolation Kit (12866-25) (MoBio, California, USA), following the manufacturers instructions. The extracted RNA was used for reverse transcription as well as macro-transcriptome birdshot sequencing library construction using Illuminas TruSeq Stranded mRNA LT Sample Prep Kit (Illuminia, USA). Each library was sequenced by Illumina NovaSeq platform (Illumina, USA) with PE150 strategy