GSM6926645: NDRG1-EGFP LMNA WT rep1-1; Mus musculus; RNA-Seq
RNA from cells were extracted from three biological replicates for each condition using TRIzol (Invitrogen) extraction and isopropanol precipitation. RNA samples were resuspended in nuclease-free H2O and further purified with RNeasy Mini Kit (Qiagen,  74106) with on-column DNase treatment. RNA purity was assessed by measuring the A260/A280 ratio using a NanoDrop. For RNA-sequencing, approximately 5.2 ng of total RNA per sample was used for library construction with the Ultra-Low-RNA-seq RNA Sample Prep Kit (Illumina) and sequenced using the Illumina HiSeq 2500 instrument according to the manufacturer's instructions for 50 bp single-end read runs.