GSM8000671: full_length_minusmet_rep2; Saccharomyces cerevisiae; RNA-Seq
Standard trizol/chloroform RNA extraction protocol was followed. The extracted RNA was analyzed with Tapestation to check their integrity. RNA with RIN (>7.5) was used for downstream analysis. Messenger RNA was captured using RNA purification beads (NEB). The eluted mRNA was fragmented and denatured for first and second strand synthesis for conversion into cDNA. The sequencing libraries were constructed using conventional protocols (NEB).