16S rDNA sequencing of chicken gut microbiome: Feed + Spirulina, sample 2
Thirty nanogram qualified DNA template and the 16S rRNA fusion primers were added for Polymerase Chain Reaction (PCR). All the PCR products were purified by Agencourt AMPure XP beads, dissolved in an Elution Buffer, and eventually labeled to finish library construction. Library size and concentration were detected by Agilent 2100 Bioanalyzer. Qualified libraries are sequenced on the DNBSeq platform according to their insert size.