single cell RNA seq of ide-cel and cilta-cel
Single cells, reagents, and 10X Genomics gel beads were encapsulated into individual nanoliter-sized Gelbeads in Emulsion (GEMs), and reverse transcription of poly-adenylated mRNA was performed inside each droplet at 53C. The cDNA libraries were then completed in a single bulk reaction by following the 10X Genomics Chromium NextGEM Single Cell 5 Reagent Kit v2 user guide. The TCR V(D)J enrichments from cDNA were performed using the 10x Genomics Chromium single cell human TCR amplification kit. 50,000 sequencing reads per cell for gene expression and 5,000 sequencing reads per cell for V(D)J were generated on the Illumina NovaSeq6000 instrument.