GSM7832494: cetuximab resistant CAL27 cytoplasmic RNA; Homo sapiens; miRNA-Seq
Total RNA was extracted using a mirVana miRNA Isolation Kit (Ambion, USA) according to the manufacturer's protocol. A total of 5 μg of total RNA per sample was used for small RNA library construction. Small RNA library construction using TruSeq Small RNA Sample Prep Kits (Illumina, USA.). Briefly, total RNA was ligated to adapters at each end. Then, the adapter-ligated RNA was reverse transcribed to cDNA, and PCR amplification was performed. PCR products ranging from 140–160 bp were isolated and purified as small RNA libraries. Library quality was assessed on the Agilent Bioanalyzer 2100 system using DNA High-Sensitivity Chips.