RNA-seq of RBM10 in LUAD cell lines
RNA-seq of RBM10 in LUAD cell lines
Cells were plated at 6cm cells and 24 hours later transfected with control or RBM10 siRNA. 48 hours after siRNA transfection, cells were directly lysed using TRIzol reagent (Ambion) for RNA extraction. Cells were grown in a humidified 37C incubator (5% CO2) in RPMI-1640 medium (Gibco) supplemented with 10% heat-inactivated FBS, 2mM L-glutamine (Gibco), 100unit/mL penicillin and 100μg/mL streptomycin (Gibco). siRNA transfections were performed using Lipofectamine™ RNAiMAX  (Invitrogen) according to the manufacturer's instructions. Total RNA was extracted from cells using TRIzol reagent according to the manufacturer's instructions (Ambion). RNA-seq libraries were prepared at the Crown Genomics institute of the Nancy and Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science using TruSeq mRNA library preparation kit (Illumina).  Libraries were quantified by Qubit (Thermo fisher scientific) and TapeStation (Agilent).