DNA-Seq of the bacterial communities: rhizosphere soil from buckwheat (Fagopyrum esculentum Moench) monoculture
Meta-barcoding analysis of the microbial community was performed based on the variable regions: V3 - V4 of the 16S rRNA gene. Specific primer sequences 341F and 785R were used to amplify the selected region and prepare the library. PCR was performed using Q5 Hot Start High-Fidelity 2X Master Mix, reaction conditions according to manufacturers recommendations