GSM8164456: SAEC, donor1, biological replicate 2, RNAseq; Homo sapiens; RNA-Seq
After 4-day treatment with compounds, cells were washed once with PBS and then trypsinized with 500 µL of TrypLE Express dissociation reagent (Gibco) and neutralized with the addition of 1 mL of growth medium. After pelleting cells with centrifugation (300g) for 3 minutes in 1.5 mL microfuge tubes, total RNA was isolated using a RNeasy kit (Qiagen). Three biological replicates were submitted for library preparation at BGI.