16S rDNA-Seq of lung metagenome: sus scrofa lung
Samples were collected, processed, and sequenced. Frozen tissue (40-70 mg) was extracted using the CTAB method, with identical processing for all negative controls. Various controls were employed to mitigate contamination during handling and processing. The 16S rRNA gene was amplified and sequenced across five regions in multiplex on the Illumina NovaSeq 6000. Reads were demultiplexed, filtered, and aligned to targeted regions using primer sequences. The SMURF method integrated read counts, optimizing results. Contamination was removed via filters, using the GreenGenes database as a reference.