RNAseq of tomato root apical meristeme
According to the manufacturer's description, the root and shoot tissues were homogenized, and total RNA was extracted for each sample using the INVITEK plant RNA mini kit (INIVTEK, Germany). RNA was fragmented, and polyadenylated mRNA was enriched by poly-A-specific reverse transcription. A specific adapter was ligated to the 5' ends, and the 3' ends were amplified by competitive PCR. MACE sequencing is based on the TrueQuant method, which reduces the amount of duplicate transcript sequencing and enables the precise comparison and identification of ultra-low expressed transcripts. Sequencing was performed on the Illumina platform