PF15 trained P0 rep 1
Adult worms (P0) trained as described on E. coli OP50 or PF15 were collected in M9 and washed several times to remove excess bacteria. Worm pellets were crushed in liquid nitrogen and transferred to an appropriate volume of Trizol LS (100 l of worm pellet in 900 uL of Trizol). Total RNA was extracted from Trizol using chloroform extraction, isopropabol and ethanol precipitation, and cleanup using the RNeasy mini kit. mRNA libraries for directional RNA sequencing were prepared using the SMARTer Apollo System and were sequenced (paired-end) on the Illumina HiSeq 2000 platform.