YY_CT
The concentration of singlenucleus suspension was adjusted to 3-4  105 nuclei/mL in PBS. Single nucleus suspension was then loaded onto a microfluidic chip (GEXSCOPE Single NucleusRNA-seq Kit, Singleron Biotechnologies) and snRNA-seq libraries were constructed according to the manufacturers instructions (Singleron Biotechnologies). The resulting snRNA-seq libraries were sequenced on an Illumina NovaSeq 6000 instrument with 150 bp paired end reads.