GSM8338814: C1; Mus musculus; RNA-Seq
The mice in the EX group were euthanized and killed by cervical dislocation 24 h after the last training. We used phosphate buffered saline (PBS) for transcardial perfusion and cadavers disinfected with 70% ethanol and placed the dissection table with the abdomen facing up. The hind leg muscles were removed and placed in the prepared petri dish, and eliminated the intermuscular fat removed [21]. The tissue samples were packaged and stored according to the requirements of each omics sequence: the size of soybean grains was required for transcription, the amount of tissue was required to reach 50-100 mg for metabolomics, >200 mg for proteomics and >500 mg for modified proteomics. The collected tissue samples were quickly frozen in liquid nitrogen and finally transferred to -80 °C for subsequent trials. First, TRIzol reagent was used to extract total RNA from muscle tissue samples and purify it to ensure that the quality and concentration of RNA samples met the requirements of subsequent sequencing. Library was constructed and sequenced based on DNBseq platform (PE150 strategy). Low-quality sequences, such as head sequence, sequence with mass score <20, original reads with N-base ratio >10% and sequence less than 25bp, were removed from the RNA sequencing results.