RNA-seq of human AML patient samples with complex karyotype
RNA-seq of human AML patient samples with complex karyotype
AML samples were collected from diagnostic bone marrow (BM) or peripheral blood (PB) aspirations at the University hospital in Heidelberg in accordance with the Declaration of Helsinki after obtaining written consent from each patient. The project was approved by the Ethics Committee of the Medical Faculty of Heidelberg (NCT-MASTER Platform S-206/2011 and S-169/2017). Mononuclear cells were isolated by density gradient centrifugation using Ficoll Paque Plus (GE Healthcare, cat # GE17-1440-03), and stored in liquid nitrogen until further use. RNA extraction and purification of cells was done using PicoPure RNA Isolation Kit according to manufacturer's instructions (Thermo Fisher, cat # KIT0214).  Whole transcriptome amplification was performed using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio, # 634889) according to the manufacturer's instructions. Tagmentation of cDNA was done using Nextera XT DNA Library Preparation Kit (Illumina, cat # FC-121-1030).