GSM7524717: aco mutant ventricle_1; Danio rerio; RNA-Seq
The heart chambers, bulbous arteriosus, and sinus venosus were removed from the adult fish and then separated into their respective tissues. Heart tissues from 20 fish were pooled and then placed in TRIZOL. RNA was harvested using phenol-chloroform separation and a PureLink RNA Micro Kit (Invitrogen).  The RNA was submitted to the CCHMC core for library generation and sequencing. Singles and paired-end sequencing was performed. RNA libraries prepared for sequencing using standard protocols