RNA-Seq of Limnephilus flavicornis silk gland
RNA isolated by TriZOL reagent was depleted of rRNA by RiboMinus Eukaryote Kit for RNA-Seq and the mRNA fraction was enriched using Dynabeads Oligo (dT)25 mRNA Purification Kit. The cDNA library was created with a NEXTflex Rapid RNA-Seq Kit and sequenced in the 2x150 nt pair-end format.