GSM7520287: RNA-seq - day 2 iPSCs IFNγ Rep 1; Mus musculus; RNA-Seq
Cells were dissociated and sorted based on SSEA1-APC and X-GFP expression. Cell pellets were frozen at -80ºC until processed. RNA was extracted from cell pellets by using a RNeasy Plus Mini Kit (Qiagen, 74136) or RNeasy Micro Kit (Qiagen, 74004). RNA libraries preparation was performed by ribosomal RNA depletion using the TruSeq Stranded Total RNA Library Preparation Kit (Illumina, 20020596) Paired-end sequencing (2 x 50 bp) on an Illumina HiSeq 2500.