Genome sequencing of Shinella sp. S4-D37
Genomic DNA was sheared to approximately 10 kb using g-TUBEs (Covaris) and further processed according to the protocol. Library preparation was performed applying the SMRTbell template prep kit 1.0 SPv3 and Barcoded overhang adapter kit- 8 A according to the Procedure and Checklist Preparing Multiplexed Microbial SMRTbell Libraries for the PacBio Sequel System. The prepared SMRTBell libraries were loaded according to the diffusion loading protocol (PacBio). The multiplexed library was loaded on one SMRT cell at a concentration of 5 pM. Movie time was 10 h per SMRT cell after immobilization for 2 h and pre- extension for 2 h. After data demultiplexing, the genome assembly was performed using the Microbial Assembly pipeline as embedded in SMRT Link version 10.1.0.119588.