mitogenome of Vulpes rueppellii
DNA was randomly sheared into short fragments, size selected to ca. 350 base pairs (bp), A-tailed, ligated to Illumina adapters (5'-AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTAT-CATT-3' and 5'-GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATGACTATC-TCGTATGCCGTCTTCTGCTTG-3'), PCR-amplified, and purified. After subsequent quantification and checks for fragment size distribution using Qubit, real-time PCR and a bioanalyzer, the library was sequenced on an Illumina Novaseq instrument using paired-end reads (2x 151 bp).