GSM7866666: DMSO_BRD4_IP; Homo sapiens; ChIP-Seq
Fixed cells were sonicated and clear cell lysate was incubated with antibody. Dynabeads was added to isolate protein-DNA complexes. DNA elute was sent to BGI for ChIP sequencing. Briefly, ChIP DNA was end repaired and purified for A-Tailing followed by adaptor ligation. Then, the adapter-ligated DNA was enriched by PCR amplification and 100-300bp PCR products were selected. DNA library preparation was qualified and DNA sequencing was performed on BGISEQ-500 platform (BGI-Shenzhen, China).