GSM6975637: Geniculate Ganglion from Egr4+/+ wild type adult 3; Mus musculus; RNA-Seq
Total RNA was extracted from isolated Geniculate Ganglia from four Egr4+/+ and four Egr4-/- mice each using the RNeasy plus micro kit (Qiagen, catalog no. 74034) according to the manufacturer instructions. Purified total RNA was first evaluated for its quantity and quality using an Agilent Bioanalyzer 2100 (Agilent genomics). A RIN (RNA Integrity Number) value of seven or higher was required to pass quality control. Only RNA samples with RIN>8 were only used for sequencing. One nanogram of total RNA was used for library preparation using the Clontech SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio USA, Inc.) and the Illumina Nextera XT DNA Library Kit (lllumina). The resulting uniquely indexed libraries were quantified and quality accessed by Qubit and Agilent Bioanalyzer. The multiple libraries were then pooled in equal molarity. The pooled libraries were sequenced with 2×150bp paired-end configuration on an Illumina NovaSeq 6000 sequencer using the v1.5 reagent kit.