GSM7968177: EPCART_WT_2; Homo sapiens; RNA-Seq
RNA from cells was isolated using Trizol (Invitrogen, Thermo Fisher Scientific), treated with RNase-free DNase set (Qiagen), and purified by Monarch RNA Cleanup Kit (New England Biolabs) according to manufacturers' protocols. The purified RNA was quantified by Qubit 4 Fluorometer (Invitrogen, Thermo Fisher Scientific) and Qubit RNA Broad Range Assay Kit (Invitrogen, Thermo Fisher Scientific), and its purity assessed by the 260nm/280nm ratio. RNA integrity was checked using 5300 Fragment Analyzer System (Agilent Technologies). Library preparation was performed using standard polyA enrichment and strand-specific library protocol, and sequencing was performed with a Novaseq6000 (Illumina) in Novogene (Hong Kong, China) for 150 bp paired­end reads. On average, 105 million cleaned reads per sample were obtained in strand-specific RNA-seq.