RNAseq of PI4P pulldown of Nuclear fraction RNA of HeLa cells
RNA extraction from the nuclear fraction of HeLa cells followed by pulldown with phosphatidylinositol 4 phosphate (PI4P) coated-beads and recovery of bound RNA with trizol,  for sequencing ribosomal RNA was removed. After RNA fragmentation, the first strand cDNA was synthesized, a second strand cDNA synthesis, end repair, A-tailing, adapter ligation, size selection, amplification, and purification