GSM7527638: RNA-WT-DMSO-rep2; Arabidopsis thaliana; RNA-Seq
For ChIP-seq experiments, 1~2 g of unopen flower buds or 2~4 g rosette leaves from the T2 lines were collected. For RNA-seq, rosette leaf tissues from 4- to 5-week-old plants, 500 uL of open flowers, 1 inflorescence, or 3 individuals of 10-day-old seedlings were harvested. For WGBS, rosette leaf tissues from 4- to 5-week-old plants, 1000 uL of open flowers, or 5 individuals of 10-day-old seedlings were pooled as one biological replicate. For snRNA-seq, 5 mL of unopen flower buds and open flowers from the same inflorescence were harvested. For MNase-seq, 0.5 g of unopen flower buds were freshly harvested. ChIP-seq and Mnase-seq libraries were prepared using the Ovation Ultra Low System V2 1-16 kit (NuGEN, 0344NB-A01) following the manufacturer's instructions. Whole genome bisulfite sequencing libraries were prepared using the Nugen Ultralow Methyl Kit. RNA-seq libraries were prepared using TruSeq Stranded mRNA kit (Illumina). SnRNA-seq libraries were prepared with 10x Genomics Chromium Single Cell 30 Reagent Kit v3. ChIP-seq, RNA-seq, snRNA-seq, WGBS, and MNase-seq