GSM8199450: Astrocyte,AGRcKO,EAE,snRNA,rep1; Mus musculus; RNA-Seq
The cells were resuspended in sorting buffer of lysis buffer of 0.2% Triton X-100, protease inhibitor (Roche, 04-693-159-001), 1 mM DTT (Sigma-Aldrich, D9779), 0.2 U/µL RNAsin (Promega, N211B), and 2% BSA (Sigma-Aldrich, SRE0036) in PBS. After incubating 5min on ice, the cells were passed through a 40 µm strainer to remove excessive debris. We generated snRNA-seq libraries using the Chromium Single Cell 3' Library & Gel Bead Kit v3.1 (10x Genomics).