MNGS of homo sapien: plasma
Simultaneous reverse transcription of purified RNA and ribosomal RNA depletion were carried out followed by second strand cDNA synthesis. Complementary DNA was purified using AMPure XP beads and loaded on an automated library prep instrument (MagicPrep NGS, Tecan Genomics) to undergo end-repair, adapter ligation and barcoding, amplification (25 cycles) and purification.