GSM8001033: vehicle, replicate 1, scRNAseq; Mus musculus; RNA-Seq
Single cell suspensions were generated from six MOC-2 tumor-bearing male mice at 24 hours after intratumoral drug or vehicle treatment as described. Cells were stained with Zombie UV Viability Dye (BioLegend, San Diego, CA) andlive cells were isolated by FACS sorting on an Aria II running FACSDiva (BD Biosciences).Each tumor sample was hash tagged withChromium Next GEM Single Cell 3ʹv3.1 Cell Multiplexing Oligo (10x Genomics, Pleasanton, CA), counted, and pooled in equal ratio prior to scRNA-seq. Indexed sequencing libraries were constructed using the Chromium Single-Cell 3′ Library Kit (Parse Biosciences, Seattle, WA) by the Baylor College of Medicine Single Cell Genomics Core and subsequently sequenced using NovaSeq X Plus PE150 by the Novogene Corporation (Sacramento, CA). Indexed sequencing libraries were constructed using the Chromium Single-Cell 3_ Library Kit (Parse Biosciences, Seattle, WA) by the Baylor College of Medicine Single Cell Genomics Core. Standard Novoseq library preps were utilized