GSM7527825: HTMEx TGF-treated sample 3; Homo sapiens; RNA-Seq
After tissue lysis, total RNA was extracted from the HTMEx using the Nucleo Spin RNA XS kit according to the manufacturer's specifications. Amplification and RNA sequencing experiments from 3.5 ng of total RNA were conducted using SmartSeq (Takara), Nextera XT (Illumina), and NovaSeq 6000 SP Reagent Kit (200 cycles) (Illumina) at iGenSeq (Institut du Cerveau (ICM), Paris, France).