GSM8528571: NCI-H660_PROX1_Sh.02; Homo sapiens; RNA-Seq
Total RNA was harvested using RNeasy Plus Mini Kit. mRNA was purified from total RNA using poly-T oligo attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP for directional library.