GSM8411420: Single nuclei form uninjured heart tissue, Un4; Mus musculus; RNA-Seq
Mice hearts were harvest at 7 days after MI. The injured heart tissue was dissected and homogenized using nuclei lysis buffer. Nuclei pellet was resuspended and filtered through 100μm and 20μm Cell Strainer (43-50000-98, PluriStrainer). Nuclei concentrations were counted using Countess II Automated Cell Counter (Thermo Fisher Scientific). Single nuclei gene expression libraries were created using Chromium Next GEM Single 'ell 3' (v3.1 Chemistry) (PN-1000121, 10x Genomics), Chromium Next GEM Chip G Single Cell Kit (PN-1000127, 10x Genomics), and Single Index Kit T Set A (PN-1000213, 10x Genomics) according to the manufacturer's instructions. Briefly, nuclei were loaded to target 10,000 nuclei to form GEMs and barcode individual nuclei into cDNA libraries. Library quality was assessed using 4200 TapeStation System and D1000 ScreenTape (Agilent) and Qubit 2.0 (Invitrogen) for concentration and size distribution.