GSM6857968: 201911G_FZH_NC; Homo sapiens; RNA-Seq
Cell density and survivability were checked for fresh PBMCs from cohort I by Count Star (Alit life science, Shanghai, China), with cell viability > 90% for each sample. Single-cell suspensions were transformed to barcoded scRNA-seq libraries using Chromium Single Cell B Chip Kit (10x Genomics, 1000074) and Single-cell 3′ Library and Gel Bead Kit V3(10x Genomics, 1000075). Then, single-cell cDNA synthesis and amplification were generated in a S1000TM Touch Thermal Cycler (Bio Rad).