16S rRNA Sequencing of Homo sapiens: infant gut
High-throughput sequencing of the hypervariable V4 region of the 16S rRNA gene, using 150 bp primers in PCR, was performed at The Centre for the Analysis of Genome Evolution and Function (CAGEF) at the University of Toronto. Primers were adapted to incorporate Illumina adapters with indexing barcodes and sequenced using MiSeq. 15 samples were multiplexed per run.