RNA-seq of homo sapien: adult female breast
3 x 105 cells per well were treated with 10 M ATO for 8 h. The total RNA was extracted using the RNeasy mini kit (Qiagen) following the manufacturers instructions. DNA digestion was performed in solution using the RNase-Free DNase from Qiagen. RNA clean-up was performed using the RNeasy mini kit (Qiagen) following the manufacturers instructions. RNA concentration and integrity were determined by the Nanodrop spectrophotometer (LabTech) and the RNA 6000 Nano kit on the Agilent Bioanalyzer 2100 (Agilent systems), respectively. Strand-specific cDNA library (polyA selected) was sequenced using the Illumina NovaSeq platform at Eurofins Genomics, Germany.