GSM8124883: day235_CapIGseq_BCL2+CK_rep1; Mus musculus; OTHER
Genomic DNA was extracted using the PureLink genomic DNA mini kit (Invitrogen, cat# K182002) following the manual. Briefly, 250 ng of DNA per sample was sheared to 250 bp segments using the Covaris LE220 and then subject to DNA library construction using the KAPA HyperPrep kit (Roche, cat# KK8502). We next followed the IDT xGen hybridization capture of DNA libraries protocol to capture the BCR VDJ fragments from the library using a custom probe set designed through the IDT xGen Hyb Panel design tool.