GSM7703389: HEK293 cells, Empty Vector replicate 1; Homo sapiens; RNA-Seq
RNA was purified using the QIAGEN RNeasy Mini Kit. Total RNA integrity was evaluated using the Bioanalyzer (Agilent). RNA-seq libraries were prepared from total RNA using the Illumina Stranded mRNA kit (Illumina). Libraries were then profiled on a TapeStation D1000 tape (Agilent) and quantified using an NGS Library Quantification Kit (Roche/Kapa) on a StepOnePlus Real Time PCR Workstation (Thermo/ABI).