GSM8474983: scRNA_HCT_DOXr2; Homo sapiens; RNA-Seq
HCT-116 cells (0.5 million) were lysed in 100 µl of lysis solution (RNAqueous™-Micro Total RNA Isolation Kit from Invitrogen). RNA was isolated and treated with DNase following the manufacturer's protocol. scRNA-Seq was performed using 10x Genomics system RNA-Seq:NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) was used for library construction. Single 50 cycles of sequencing data were acquired on NovaSeq6000 (Illumina). scRNA-Seq:Libraries were prepared using Chromium Single Cell 3′ Reagent Kits (10X Genomics) according to the manufacturer's protocol. With double 10bp index cycles, 28 forward and 90 reverse cycles were run on NovaSeqXplus machine (Illumina).