GSM7653636: B cells, spleen, TFEB-WT, rep1; Mus musculus; RNA-Seq
Spleen were passed through 40µm cell strainer to generate single-cell suspensions. Subsequently, ells were were sorted according to indicated marker, pelleted by centrifugation, subsequently resuspended in TRIzol reagent (Invitrogen) and stored at -80°C. Total RNA was purified from sorted cells using the TRIzol protocol (Invitrogen)extracted with RNA-seq libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) with minor modifications in ligation and amplification (protocol from Schattling, B., Engler, J.B., Volkmann, C. et al. Bassoon proteinopathy drives neurodegeneration in multiple sclerosis. Nat Neurosci 22, 887–896 (2019). https://doi.org/10.1038/s41593-019-0385-4)