GSM8019267: TLR5+ macrophages_2; Mus musculus; RNA-Seq
Total RNA was extracted using Trizol (Invitrogen). Total amounts and integrity of RNA were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). RNA libraries for RNA-seq were prepared using NEBNext® Ultra™ RNA Library Prep Kit following manufacturers protocols.