GSM7520388: PH-LHD_3; Homo sapiens; RNA-Seq
Snap-frozen PA specimens were subjected to TRIzol-RNA extraction. RNA quality was assessed by measuring the RNA integrity number (RIN) using a Fragment Analyzer HS Total RNA Kit (Advanced Analytical Technologies, Inc.). Library preparation for total RNA-Seq was performed using TruSeq™ Stranded Total RNA with Ribo-Zero Gold (Illumina, Cat. Nr. RS-122-2301) starting from 1000 ng of total RNA. The size range of the final cDNA libraries was determined by applying the SS NGS Fragment 1- to 6000-bp Kit on the Fragment Analyzer (average 340 bp). Accurate quantification of cDNA libraries was performed by using the QuantiFluor™ dsDNA System (Promega).