GSM7780675: WT Col.0.1; Arabidopsis thaliana; RNA-Seq
RNA for RNA-seq was extracted with the RNeasy Plant Mini Kit (Qiagen) from inflorescences and flowers in post-anthesis, adding the DNase to the column with the RNase-Free DNase Set (Qiagen) according to manufacturer's instructions. RNA concentration and purity were verified using a NanoDrop Spectrophotometer ND1000 and a Bioanalyzer 2100 (Agilent Technologies). The cDNA libraries preparation, sequencing and analysis were made by Novogene (UK) Company Ltd. using Illumina Novaseq6000 system with a PE150 strategy (pair end – 150 pb simple ends).