2_MS1.26
Genomic DNA was extracted from HEK293T cells by Ezup Column Animal Genomic DNA Purification Kit (Sangon). The target genomic loci were amplified using target-specific primers. Amplicon-seq libraries were prepared by using the VAHTS Universal DNA Library Prep Kit for Illumina (Vazyme) according to the manufacturers instructions. Finally, the purified library was subjected to Illumina NovaSeq 6000 by Haplox (Jiangxi).