GSM7967294: RNA-seq_HeLa_NT_Rep2; Homo sapiens; OTHER
RNA was purified using Sepasol-RNA I Super G with phenol-chloroform extraction and ethanol precipitation method. RNA-seq libraries were prepared using the TruSeq Stranded Total RNA Library Prep Gold (Illumina) following manufacturer's instructions. Libraries were checked for quality and quantity using Bioanalyzer. Equimolar concentrations of libraries were pooled, requantified and sequenced as paired-end reads on the Illumina NovaSeq 6000 instrument. 4sU RNA-seq