GSM8262772: Vagal sensory neurons-F; Mus musculus; RNA-Seq
Thirty vagus nerve ganglia were collected from eight male and seven female Rosa26-eGFPf  mice injected with AAVrg-Cre into the liver four weeks after viral injection.16 vagus nerve ganglia from males and 14 vagus nerve ganglia from females were flash-frozen. Individual tissue samples were divided into separate pools for males and females. These pooled samples were subsequently homogenized and lysed using Triton X-100 in RNase-free water to isolate the nuclei. For nuclear isolation, we followed the 10x genomics protocol, CG000124 (Rev. F). The isolated nuclei were diluted to 700 nuclei/µl for standardized 10x capture. The 10x Genomics single cell protocol was immediately proceeded using 10x Genomics Chromium Next GEM 3' Single Cell Reagent kits v3.1. Single nucelus RNA sequencing