GSM7081875: 42-21E1, KO; Mus musculus; RNA-Seq
PureLink TM, RNA minikit, Invitrogen. Cat no.12183018A. RNA-Seq libraries for poly-A-enriched fractions were generated from total RNA using TruSeq Stranded mRNA Library Prep Kit and TruSeq RNA Single Indexes kits A and B (Illumina, San Diego, CA), according to manufacturer's instructions. High throughput sequencing of 100 bp paired-end reads was carried out on an Illumina HiSeq 2500 platform  (GenomEast Platform, IGBMC, Illkirch, France).