ONT long-read sequencing of Leptopilina malgretoutensis
DNA was extracted from a pool of ~100 adult female wasps using a Gentra PureGene Tissue Kit (Qiagen). 3 ug of DNA was end prepped using NEBNext FFPE Repair Mix (M6630), NEBNext Ultra II End repair/dA-tailing Module (E7546), and NEBNext Quick Ligation Module (E6056) and subsequently library prepped using the ONT supplied SQK- LSK110 kit. Sequencing took place on an Oxford Nanopore Minion over 3 days with nuclease wash followed by reloading after 24 hours. Fastq files were generated from the Oxford Nanopore Minion instrument and called with Guppy v6.1.5. We used the GPU enabled version of guppy in concert to enable live basecalling with the fast base calling model and for post-hoc base calling with the super (sup) accuracy model called via Minknow v22.05.5.