GSM8443657: ENEG_NASH8; Homo sapiens; RNA-Seq
Total RNA isolation of FFPE liver biopsies and library preparation were performed as described previously (Montanari NR et al. Multi-parametric analysis of human livers reveals variation in intrahepatic inflammation across phases of chronic hepatitis B infection. J Hepatol. 2022 Aug;77(2):332-343. PMID: 35218813). Core needle liver biopsies were sectioned, deparaffinized, and total RNA was isolated using the RNeasy FFPE Kit (Qiagen, Germany) following the manufacturer's instructions. Prior to sequencing, rRNA was depleted. Library preparation and sequencing were performed by EA Genomics (Q2 solutions, USA) on the Illumina HiSeq platform (100 bp paired-end reads, 50 million reads per sample) and by GenomeScan (Leiden, The Netherlands) on the Illumina NovaSeq 6000 (150 bp paired-end reads, 30 million reads/sample) using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina sequencing. All samples passed the library preparation QC. Library preparation and sequencing were performed by EA Genomics (Q2 solutions, USA) and by GenomeScan (Leiden, The Netherlands).