GSM7835972: 786-O, Mit AQC, Inp; Homo sapiens; Mus musculus; ChIP-Seq
Elution and decrosslinking of DNA was performed in Elution Buffer (1% SDS, 0.1M NaHCO3, 100 mM NaCl) with addition of 20 ug/ml RNase A at 65°C overnight. DNA was then extracted with QIAquick PCR Purification Kit (Qiagen) after treatment with 100 ug/ml proteinase K at 55°C for 1–2 h. Libraries were prepared from 1ng of immunoprecipitated DNA or Inputs with the MicroPlex v2 kit (Diagenode), according to manufacturer's instructions.