GSM8406363: HepG2R-250nm-1; Homo sapiens; RNA-Seq
Total RNA was extracted using RNeasy Mini Kit (Cat# 74106, Qiagen)following the manufacturer's instructions and checked for a RIN number to inspect RNA integrity by an Agilent 2100 Bioanalyzer (Agilent technologies, Santa Clara, CA,US).Qualified total RNA was further purified by RNAClean XP Kit (Cat A63987, Beckman Coulter,Inc.Kraemer Boulevard Brea, CA,USA)and RNase-Free DNase Set(Cat#79254, QIAGEN, GmBH, Germany). According to the experimental instructions, the purified Total RNA was subjected to the steps of mRNA separation, fragmentation, first-strand cDNA synthesis, second-strand cDNA synthesis, terminal repair, 3' terminal A addition, linker connection, enrichment, etc., to complete the construction of sequencing sample library.