GSM8128795: ChIP-WT_CTCF-rep2; Mus musculus; ChIP-Seq
ChIP assay was performed using Active Motif's ChIP assay kit (53035) according to manufacturer's instructions with slight modifications Briefly, a total of 1 x 107 cells were harvested and cross-linked with paraformaldehyde. Then cells were lysed and digested with shearing enzyme followed by sonication. Anti-TRIM28 antibody (Active Motif, 61173), Anti-CTCF (CST, 3418), Anti-SMC1 (Bethyl Laboratories, A300-055A), and Anti-H3k27ac (Active motif, 39133) was added to supernatant followed by Dynabeads Protein A (LifeTechnologies) pulling down target fragment. The precipitated DNA was then washed, eluted, de-crosslinked and purified for realtime qPCR analysis or sent to BGI Genomics for cDNA library preparation and sequencing. Every sample has two replicates.