Illumina HiSeq 2500 paired end sequencing; Multi-omic dissection of heat stress memory responses in Brachypodium distachyon - RNA-seq data
Multi-omic dissection of heat stress memory responses in Brachypodium distachyon - RNA-seq data
Sample collection was performed by Dr. Richard Poire, ANU Plant organ samples were frozen in liquid nitrogen and stored in -80C before grinding with liquid nitrogen and extraction 100mg of samples with Sigma Spectrum Plant Total RNA kit. Four B. distachyon Bd21-3 seeds were sowed in the soil and vernalized for 5 days in the 4℃ cold room. After vernalization, the seeds were grown at 25℃, 50% relative humidity, 100 μE*m-2*s-1 light intensity, and with a day/night cycle of 24/0 hours B. distachyon Bd21-3 seedlings were divided into an acclimated group and a non-acclimated group respectively after 21 days from vernalization. Before a 1-day 39℃ heat stress treatment, the acclimated group went through a 1-day 39℃ heat priming and a 2-day recovery, while the non-acclimated group kept at normal temperature for 3 days. In each group, leaves of 3 biological replicates at 10 important time points were cut and immediately stored in a -80℃ freezer. N/A RNA extraction performed with Sigma Spectrum Plant Total RNA kit Directional mRNA library preparation (poly A enrichment)