GSM8591092: O4, female, BPA, ATAC, rep5; Mus musculus; ATAC-seq
Brain O4+ oligodendrocytes were isolated by magnetic cell sorting (Miltenyi). Nuclei were isolated from 50,000 cells and chromatin was tagmented during 30 min at 37°C using Tn5 (Illumina). DNA fragments were purified using MinElute columns (Qiagen). Purified DNA fragements were subjected to a two-step amplification and tagging process with NEBnext High-Fidelity 2× PCR master mix (New England Biolabs). The resulting amplified DNA underwent double purification using 1.8 volumes of NucleoMag NGS Clean-up and Size Select beads (Macherey Nagel). The final libraries were checked for quality, quantified, multiplexed and sequenced on an Illumina NextSeq500 system (Illumina, San Diego, California, USA) at IGenSeq Platform (ICM, Paris, France).