RNA-Seq of human: adult female WBC
Peripheral blood samples were collected from participants diagnosed with migraine without vertigo (MOV), vestibular migraine (VM), and Meniere's disease (MD) as well as healthy controls. RNA sequencing was performed to compare gene expression profiles among these groups. Peripheral blood RNA was extracted using TRIzol, and libraries were prepared with the Hieff NGS Ultima Dual-mode mRNA Library Prep Kit. Sequencing was conducted on an Illumina NovaSeq platform, generating 150 bp paired-end reads. Bioinformatic analyses, including differential expression, functional enrichment, and protein-protein interaction analysis, were performed to identify molecular mechanisms underlying these disorders.