GSM8705418: TMD8_JIB04_500nM_Rep1; Homo sapiens; RNA-Seq
Total RNA from JIB-04 or DMSO treated OCI-Ly1 and TMD8 cells were isolated using the RNeasy Plus Micro kit (Qiagen cat. no. 74034) per the manufacturer's protocol. Purified RNA was subjected to ribosomal depletion, using the NEBNext rRNA Depletion Kit (New England Biolabs cat. no. E7770L). RNA-Seq libraries were prepared in technical triplicate using the NEBNext Ultra RNA II Library Prep Kit (New England Biolabs cat. no. E6310L) and paired-end sequenced with 101 cycles each read on an Illumina HiSeq.