GSM8267018: PEO4, PITX1, ChIP; Homo sapiens; ChIP-Seq
Cells were fixed following Active motif's ChIP Cell Fixation Protocol (https://www.activemotif.com/documents/1848.pdf).Chromatin was prepared using RIPA buffer containing 10 mM Tris-HCl, pH 7.6, 1 mM EDTA, 0.1% SDS, 0.1% Na-Deoxycholate, 1% Triton X-100 and 100 μL of protease inhibitor cocktail (Millipore Sigma #11836170001 Roche) and sonicated using QSonica apparatus for optimal fragmentation. Pulldown was set up using respective antibodies for overnight. DNA was extracted from pulled down chromatin. Libraries were prepared using 5 ng of DNA using Ovation® Ultralow System V2 (TECAN #0344-32) following manufacturer's protocol.