GSM8171871: DRGneurons_Vehicle_sample01; Mus musculus; RNA-Seq
Total RNA was isolated using a single cell RNA extraction kit (PicoPure, Thermo Fisher). Quantity of total RNA was determined using Invitrogen's Qubit HS assay and quality was checked using Agilent's bioanalyzer First strand cDNA was prepared and amplified from 1 ng total RNA using SMART Seq v4 Ultra Low Input RNA Kit for sequencing (TaKaRa) according to the manual compatible with Illumina NGS. Barcoded sequencind libraries were subsequently prepared using the low input library preparation kit (TaKaRa). The Sv4 PLUS kit includes first stand cDNA synthesis kit using SMART technology and library preparation kit that incorporates enzymatic fragmentation and stem-loop adapters to construct high-quality, Illumina-compatible libraries from the cDNA synthesized with the core kit. Concentration was determined using Invitrogen's Qubit HS assay and fragment size was analyzed on Agilent's 2100 Bioanalyzer on a HS DNA chip.