fecal mycobiome of mus musculus: various yeast-colonized conventional mice
Samples (~50 mg) were suspended in 50 mM Tris buffer (pH 7.5) supplemented with 1 mM EDTA, 0.2% b-mercaptoethanol, and 1000 units/mL of lyticase (Sigma-Aldrich) followed by incubation at 37 C for 30 min. After incubation, genomic DNA was extracted from ~50 mg of samples using a Quick-DNA Fecal/Soil Microbe Microprep Kit (Zymo Research) according to the manufacturers protocol. The ITS1 region of the fungal DNA was amplified using the Phusion High-Fidelity DNA polymerase (Thermo Scientific) with ITS1-F/ITS2 primer pair. The amplicons were sequenced on Illumina MiSeq 2 _ 300 bp paired-end sequencing.