RNA-seq
RNA was purified with the TRIzol LS (Invitrogen) protocol along with aqueous phase cleanup using Zymo RNA clean & concentrator-5 kit. RNA-seq libraries were created utilizing the Takara SMART-Seq v4 Ultra Low Input RNA kit using 2 ng of RNA. Libraries were sequenced using Illumina NextSeq1000 (paired end, read lengths 50 bps).