GSM8336828: 29TKN; Homo sapiens; RNA-Seq
During the CABG procedure tissue samples are extracted from athymic adipose tissue and epicardial adipose tissue by a qualified cardiosurgeon. All tissue material was suspended in RNAlater soluton and stored frozen at -80°C. RNA was extracted from frozen tissue samples using RNeasy Mini Kit (Qiagen) following manufacturer's protocol. Libraries were constructed using TruSeq Standard mRNA Librery Prep (Illumina, California, USA) following manufacturer's protocol. The RNAseq procedure was performed with NextSeq 500/550 High Output Kit v2.5 (300 Cycles; Illumina, California, USA) in accordance with the protocol provided by the manufacturer.