Gene expression changes in S. rhizophila in a dual-species biofilms with B. licheniformis compared to its monoculture biofilm
Bacterial dual-species biofilm comprising S. rhizophila and M. lacticum was grown on stainless steel coupons in the presence of BHI using 6-well microtiter plates at 37C for 24 h. Biofilms were harvested and RNA was extracted using total RNA extraction kits from Qiagen. Sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) following manufacturers recommendations and sequencing was performed on an Illumina platform and paired-end reads were generated. Both building index of reference genome and aligning clean reads to reference genome were used Bowtie2.