GSM8172288: MII_2; Mus musculus; RNA-Seq
Oocytes were collected at the indicated time after DMSO and BYBX treatment (10 oocytes per sample). Each sample was lysed using 4 μl lysis buffer (0.2% Triton X-100, RNase inhibitor, dNTPs, oligo-dT primers, and 100 pg external RNA controls consortium (ERCC) mRNA spike-in) and immediately used for cDNA synthesis based on Smart-seq2 method. Sequencing libraries were constructed from 500 pg of amplified cDNA using the TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme; TD503) according to the manufacturer's instructions. Barcoded libraries were pooled and sequenced on the Illumina HiSeq X Ten platform using 150 bp paired-end reads.