RNA-seq of leaf tissue from four genotypes of Lathyrus sativus infected with the Erysiphe pisi
RNA-seq of leaf tissue from four genotypes of Lathyrus sativus infected with the Erysiphe pisi
The infected leaves were collected before inoculation and at 12, 48 and 72 hours after inoculation using liquid nitrogen. The samples were stored at -80 ºC until RNA extraction. Seedlings were grown in 0.5 L pots containing 250 cm3 of peat in a growth chamber at 20°C, with a 12-h light/12-h dark photoperiod until 14 days old. Fourteen-days old seedlings were inoculated with Erysiphe pisi isolate Ep-CO-01 using a settling tower to ensure uniform conidial deposition of 8 conidia/mm2. The samples 0 hai were not inoculated. Total RNA was isolated using the GeneJETTM Plant RNA Purification Mini Kit (ThermoScientificTM, Massachusetts, USA) according to the manufacturer's instructions.  The RNA library construction was carried out using a Stranded mRNA Library Preparation Kit (Roche/KAPA mRNA HyperPrep kit).