GSM8264925: MOLM13, VEH, ATAC-seq Rep1; Homo sapiens; ATAC-seq
50,000 MOLM13 cells treated with either 5µM DB2115 or vehicle were subject to nuclei isolation with ATAC resuspension buffer (containing 0.1% NP-40, 0.1% Tween20 and 0.01% Digitonin) before transposition was performed using Tn5 transposase in TD buffer (Illumina Tagment DNA kit). Transposed DNA was purified using a Minelute PCR purification kit (QIAGEN) before eluted DNA was amplified via PCR with custom PCR primers and cycle number was determined using a KAPA Library Quantification kit (KAPABiosystems).