GSM6886470: BT0510, Osmotic stress  - alcohol stress, biological replicate 1; Saccharomyces cerevisiae; RNA-Seq
Total RNA from three biological replicates for each treatment was extracted using the Qiagen RNeasy Mini kit (Qiagen, Hilden, Germany) following the manufacturer's instructions. Poly-A tailed RNA was captured, and reverse transcribed to complementary DNA (cDNA), which was used for library preparation by the Stranded Total RNA Prep (Illumina, USA) following the manufacturer's protocol.