GSM7890185: Normal_WT_5468; Mus musculus; ATAC-seq
Tissues were dissociated into single cells by enzymatic digestion using procedure described previously in Li, et al., Cell Reports. 33, 108566 (2020). Cell pellets were tagmented using the enzyme and buffer provided in the ATAC-Seq Kit (Active Motif). Tagmented DNA was then purified using the MinElute PCR purification kit (Qiagen), amplified with 10 cycles of PCR, and purified using Agencourt AMPure SPRI beads (Beckman Coulter).