GSM8561732: MDA-MB-231/RR, control, 1; Homo sapiens; RNA-Seq
The cells were incubated until confluency was approximately 70–90% and then harvested following dislodging of the cells by trypsin-EDTA. The harvested cells were centrifuged at 1,000 g, the medium was removed, and the cells were washed twice with 1× PBS. Following the final wash, the cells were resuspended in 0.1× PBS and aliquots were immediately frozen in liquid nitrogen. The filtered dataset was normalized using relative log expression to correct for variations in library sizes among the samples.