GSM8080220: ATAC-Seq, shCHD9, rep1; Homo sapiens; ATAC-seq
50,000 viable cells were resuspended in 50 uL cold ATAC-Resuspension buffer(1 M Tris-HCl, pH=7.4; 5 M NaCl; 1 M MgCl2) supplemented with 0.1% NP40, 0.1% Tween-20, and 0.01% digitonin and incubate on ice for 3 minutes. The cell lysate was washed with 1 mL of cold ATAC-RSB, pelleted, and resuspended in 50 μL of transposition master mix, which consisting of 2x TD buffer (20 mM Tris-HCl, pH=7.6; 1 M MgCl2; 20% dimethyl formamide), transposase (Illumina, 20034197), PBS, 1% digitonin (Millipore, 300410), 10% Tween-20, underwent a 37°C incubation for 30 minutes with 1000 RPM mixing. The MinElute PCR purification kit (Qiagen, 28004) was employed for fragment cleanup. Sequencing libraries were constructed using 1 x NEBnext PCR master mix and the custom primers described by Buenrostro et at (PMID: 24097267)