GSM8264934: MOLM13, VEH, 12hr, ChIP-seq; Homo sapiens; ChIP-Seq
MOLM13 cells were treated with 5µM DB2115 for 12hr before cells were crosslinked in 0.5% formaldehyde for 7 minutes at room temperature. Samples were sonicated for 12 minutes to generate fragments below 1kb before being incubated with 5µg of PU.1 antibody (Santa-cruz, sc-352) Library preparation was carried out using NEB Ultra II DNA Prep Kit (NEB) following the manufacturers recommendations.