GSM8529583: AFF4 ChIP, NIPBLmut; Homo sapiens; ChIP-Seq
Chromatin immunoprecipitation was performed as detailed in Nakato et al. (Nature Communications (2023)14:5647). Briefly, human culture cells were crosslinked with 1 % formaldehyde for 10 minutes at room temperature. Chromatin was then sheared by sonication (Branson Sonifier 250D) and combined with mouse spike-in chromatin. The immunoprecipitation was performed overnight at 4°C with an antibody conjugated to Dynabeads Protein A or B (Thermo Fisher Scientific, 10002D or 10004D). After completing the immunoprecipitation and reversing crosslinks, the DNA was purified. Sequencing library was prepared for sequencing using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, E7645) according to manufacturer's instructions.