16S rRNA (V1-V9) of tomato rhizosphere
Following DNA extraction using a PowerSoil Pro kit, we sequencing with PCR the V1-V9 region of the 16S rRNA gene. We used the 27F and 1492R primers and used the QIAgility machine to prepare mixtures. Amplification occurred in a Roche LightCycler and ethanol was used to purify PCR products. PCR products were pooled and ligated. Samples were sequenced in a minION flow cell using MinKNOW software.