GSM8076224: hNSPCs in hEC CM; Homo sapiens; RNA-Seq
Cells for single-cell RNA sequencing were prepared on the same day using the same cell sources for each condition to reduce batch effects. Cells were dissociated, resuspended in 0.4% BSA and a cell strainer was used to ensure single cells. Library was constructed per manufacturer's protocol (10x Genomics Chromium Single Cell 3' Reagent Kit (v3.1 chemistry)