GSM8781778: late stage of differentiation_2D_3; Homo sapiens; RNA-Seq
Nucleic acid extraction and purification were performed by RNA Extraction Kit (Qiagen). 500 picogram RNA was used from each sample for cDNA generation using the Takara Smart-Seq v4 Ultra Low Input RNA kit (Takara Bio USA) following manufacturer's instructions, with 15 cycles of cDNA amplification. Starting from 500 cDNA picogram, libraries were prepared through DNA library prep Illumina kit (Illumina), following manufacturer's instructions.