GSM8498973: S133A-CREB cells, CREB, ChIP; Mus musculus; ChIP-Seq
Cells were cross-linked with 1% formaldehyde at room temperature for 10 min. After micrococcal nuclease digestion, chromatin was sonicated into 150-900 bp DNA fragments. NGS libraries were prepared using VAHTS Universal DNA Library Prep Kit for MGI (Vazyme Biotech, Nanjing ,China) according to the manufacturer's protocol. All DNA libraries were quantified by Qubit Fluorescence Quantification/Enzyme Labeling Instrument, and the length distribution range of the products was quantified, which required that the total amount meets the requirements and there was no contamination of adaptors and large fragments. After library construction was completed, Qubit was used for quantitative quality control, and sequencing was performed after the quality of the library passed.