GSM8558828: NPC_Setd2KO_RNA_r2; Mus musculus; RNA-Seq
Total RNA was isolated from NPCs using the RNeasy Plus mini kit (Qiagen). RNA integrity was measured using a model 2100 Bioanalyzer (Agilent). PolyA-tailed mRNAs were isolated and enriched using NEB Next Poly(A) mRNA Magnetic Isolation Module according to manufacturer's instructions. Libraries for 1 μg mRNA were prepared using NEB Next UltraTM II Directional RNA Library Prep Kit for Illumina.