GSM8219297: RNAseq_dKO_MI503_1; Mus musculus; RNA-Seq
In RNAseq, total RNA was extracted with Trizol reagent following the manufacture's instruction. ChIPseq was performed using standard ChIP protocol (Shipra Shukla, et al. Cancer Cell, 16 November, 2017). Ten million cells were used for each antibody. CutRun was performed using standard protocol (Peter J Skene, et al. Nature Protocols, 12 April, 2018). For each WT and Kmt2c/d KO group, 250K cells were used in each reaction. ATACseq was performed using standard protocol (Jason D Buenrostro, et al. Natur Methods, 06 October, 2013). For each control and Kmt2c/d KO group, 50K cells were proceessed for transposase treatment. RNAseq libraries were prepared using standard Illumina polyA library preparation protocol (TruSeq Stranded mRNA LT Kit, catalog # RS-122-2102, 8 cycles of PCR). ChIPseq, ATACseq, and CutRun libraries were prepared using KAPA HTP Library Preparation Kit (Kapa Biosystems KK8234) according to the manufacturer's instructions. RNAseq:poly (A) capture; CurRun, ATACseq, and ChIPseq: epigenetic library.