RNA-Seq of Listeria monocytogenes 6179 Rli47 deletion mutant: logarithmic phase, 20C
RNA extraction was performed with physical lysis (Lysing Matrix E) using thte Invitrogen Purelink RNA Mini Kit and a Bead Mill 24 Homogenizer. A DNase digest was performed using the Turbo DNA-Free kit. Quality was validated using the Agilent RNA Nano 6000 Bioanalyzer Assay. RNA was submitted to the ISU DNA Facility for ribosomal RNA depletion, library preparation, and sequencing.