GSM8132020: ucMSC_Old_Hic; Homo sapiens; Hi-C
About 1 million MSCs from both PD 12 and PD 32 were fixed with 1% formaldehyde and incubated at room temperature for 10 minutes, then quenched with 2.5M glycine for 5 minutes. The cells were washed with PBS twice followed by brief centrifugation. MSCs were then lysed with Hi-C lysis buffer 93 and digested with MboI (NEB R0147S) overnight. After DNA labeling, proximity ligation, crosslink reversal, and biotin pull-down, target DNA were extracted. Sequencing library of extracted DNA were performed as described in Lieberman-Aiden, E. et al., 2009