GSM8529182: G1B, pif1-m2 dna2; Saccharomyces cerevisiae; RNA-Seq
RNA was extracted using the miRNeasy Mini Kit (Qiagen, #217004) according to manufacturer instructions. Briefly, cells were lysed in a bead miller with acid-washed glass beads and Qiagen Buffer RLT. One volume of 70% ethanol was added to the homogenized lysate and the sample was purified using the RNeasy spin column and eluted in RNAse-free water The cDNA library preparation and sequencing were conducted by Novogene following manufacturer's protocols using a paired-end 150 bp sequencing strategy on the Illumina NovaSeq 6000 platform