GSM6997581: MG1655_CmR_SpS_clone2; Escherichia coli; OTHER
Genomic DNA was purified using Sigma kit NA2120 from total bacterial culture after 6 passage at 43 °C and from 2 separate single clones spectinomycin sensitive chloramphenicol resistant from this bacterial culture. 500 ng purified gDNA were treated with NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina E7805S and barcoded using NEBNext Multiplex Oligos for Illumina® (Dual Index Primers Set 1) E7600. gDNA libraries were sequenced using the HiSeq2500 platform (Illumina) in the rapid run mode (50-nucleotide single-end reads).