GSM8561453: Tumor CDDO biol rep 2; Homo sapiens; RNA-Seq
Total RNA was extracted from mouse tissues and tumors using the Nucleospin RNA kit (Macherey-Nagel). RNA was submitted to the NGS Core Facility of the German Cancer Research Center where the high-throughput sequencing including library preparation and data quality control was performed. Sequencing libraries were prepared using TruSeq Stranded mRNA Library Prep Kit (Illumina) and IDT Unique Dual Indexes for Illumina (Integrated DNA Technologies) and sequenced using NovaSeq 6000 sequencing system (paired-end mode, read length 100 bp, S4 flow cell; Illumina), yielding on average 52 million reads per sample (32–76 million).