Amplicon of homo sapiens: LINE flanks in matched norm
The procedure for the preparation of LINE flanks libraries consists of 6 steps: DNA fragmentation with restriction enzyme; ligation of universal suppression adaptors; nested PCR with primers specifit to LINE L1HS and L1PA2 and suppression adapter; DNA end repair and A-tailing; ligation of universal sequencing adapter; library amplification with unique dual-indexed primers.