GSM8079993: B16F10 cells, shNC-1; Mus musculus; ATAC-seq
5 × 104 cells were resuspended in ice-cold nucleus lysis buffer (10 mM Tris pH 7.4, 10 mM NaCl, 3 mM MgCl2, and 0.1% IGEPAL CA-630) and centrifuged at 500 × g for 10 min at 4 °C. The supernatant was discarded, and the transposition reaction was performed using the Tn5 transposase at 37 °C for 30 min. DNA was purified using a MinElute PCR Purification Kit (Qiagen, Redwood, CA, USA), and the resulting DNA was PCR amplified. The selection of size and purification of DNA fragments were done using the AMPure beads. Size distribution and molarity of the sequencing library were determined by Qubit (Thermo Fisher Scientific, Beverly, MA). Samples were performed in replicates and sequenced on an Illumina PE150 (Illumina) platform. Reads were mapped to the mm10 reference genome