GSM8783282: IHMGECs, pulldown by anti-Histone H3, biol rep 2; Homo sapiens; ChIP-Seq
Chromatin immunoprecipitation (ChIP) assay was conducted following the manufacturer's instructions for SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) (9003, Cell Signaling) For downstream NG-sequencing DNA library construction, we used the Kapa Roche HyperPlus kit (7962428001, Roche) to generate the ChIP-seq libraries, and then the samples were sequenced on the Illumina NextSeq 550 sequencer platform with the SE-75 sequencing protocol, following the manufacturer's instructions.