GSM8591222: cse_noTrp+BSO_exp2__biol_rep_2; Mus musculus; RNA-Seq
To study transcriptional response in mouse tissues, total RNA was isolated from the homogenates by trizol (1:10 ratio) method. mRNA was purified by NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB E7490S) from Turbo-DNAse treated total RNA. A NEB Next® Ultra Library Preparation Kit (NEB E7530S) was used to prepare 0.5 µg of total RNA for RNA-seq.