Whole-genome sequencing of Escherichia coli population isolated from: lab strain DM09
The library construction and sequencing were performed at the genomics facility at Gulbenkian Institute of Molecular Medicine (Portugal). Nextera XT kit from Illumina was used for library construction. Briefly, a sample of mice feces was plated on LB agar and allowed to grow till ~1000 isolated colonies were visible. These colonies were then pooled and DNA was extracted for whole genome sequencing in a NextSeq 500 Sequencer