DNA-seq of whole-genome-amplified DNA from the 2nd ampule of the 1916 bacterial vaccine using the MiSeq machine
The DNA isolated from the supernatant and pellet fractions of the 2nd ampule of the 1916 mixed bacterial vaccine was amplified using Whole Genome Amplification (WGA) with the REPLI-g Advanced DNA Single Cell Kit (Qiagen, Germantown, MD). Illumina sequencing libraries were constructed from the amplified DNA using the ThruPLEX DNA-Seq Kit (Takara Bio USA, San Jose, CA) and sequenced on the Illumina MiSeq.