GSM8404773: P60 cortical astrocytes rep3; Mus musculus; RNA-Seq
RNA was isolated from cells with Direct-zol RNA Microprep kit (Zymo Research, #R2060) following the manufacturer's instructions Total RNA was reverse transcribed into the first-strand cDNA samples using SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio USA, Mountain View, CA, USA). PolyA enrichment was conducted to enrich all the coding RNA. The cDNA samples were then fragmented, end-repaired, A-tailed, and ligated with adaptors. After size selection and PCR enrichment, sequencing was performed on a NovaSeq instrument (Illumina).