GSM8782085: IE_SE5_5; Homo sapiens; OTHER
After incubation and hybridization of the GeoMx reagents along with visualization reagents to slide-mounted formalin-fixed paraffin-embedded tissue, the DSP scans the slides and presents a high-quality image for region of interest (ROI) selection guided by immunofluorescent marker positivity. The oligonucleotide tags are released from user-selected regions of the tissue with focused UV light based on the user region and segmentation selections. The digital micromirror device of the DSP instrument tunes the UV light with 1-μm resolution, allowing great flexibility for region selection including irregularly shaped and noncontinuous segments. library was sequenced on Illumina Novaseq6000 sequencer with paired end 50nt mode