GSM8132015: ucMSC_Old_CTCF_ChIP; Homo sapiens; ChIP-Seq
PD 12 and PD 32 MSCs were fixed with 1% formaldehyde. As described in McCauley, B.S. et al. 2021, fixed cells were lysed and chromatin was sonicated to approximately 400 bp. A total of 10μL of cell lysate (equivalent to 100,000 cells) was used as initial input for both CTCF and YY1 ChIP. The True MicroChIP Kit (Diagenode, C01010130) was used for the target molecule extraction with the antibody of CTCF (AbCam #ab190237, lot GR3206274-4) and YY1 (Active Motif #61311, lot 34614003), respectively. Sequencing libraries were prepared using the MicroPlex Library Preparation Kit v2 (Diagenode, C05010014) following manufacturer's instructions