GSM8443749: In Vitro SreA FL Cassette transcript, 1M7, primer-MaP, Replicate 1; Listeria monocytogenes; OTHER
RNAs were buffer exchanged and/or purified by centrifugation filter or column preparation before library preparation For Switch-MaP, RNAs were ligated to a 3' end adapter including an RT primer binding site and cDNA was generated by MaP RT with Superscript II including a template-switching oligonucleotide. For primer MaP experiments, gene or structure cassette-specific RT primers were used in MaP RT with Superscript II excluding template-switching oligos. Final sequencing libraries were generated by PCR using primers complentary to either  3' adapter and template-switching oligo sequences or gene/cassette-specific sequences, with additional indexing barcodes included in primers.