GSM7835906: 48hr infected 3; Homo sapiens; ncRNA-Seq
Total RNA was extracted using miRNA easy kit (Qiagen) Total RNA was pretreated with tRF/tiRNA pretreatmet kit (rStar) followed by cDNA synthesis, and PCR amplification, fragments were size-selected on 8% TBE-PAGE gel.  RNA sequencing on an Illumina platform generated FASTQ files. Reads were quality controlled, identified, and quantified using MINTmap database, focusing on exclusive tRNA fragments to avoid ambiguity. Reads with <10 read count were discarded and differential expression analysis was performed through DEseq2.