GSM7835279: muscle_heterozygous_rep4 [S044_18]; Mus musculus; RNA-Seq
Total RNA from tissue was isolated using Qiagen RNeasy kit (Qiagen, GmbH Hilden, Germany) following manufacturer's protocol. The quantity of the RNA was determined on a Nanodrop ND-1000 (Thermo Scientific, Rockford, IL, USA) Libraries were prepared using the Universal Plus mRNA-seq with NuQuant kit (Tecan) as recommended by the manufacturer. Libraries were quantified with NuQuant, quality checked using a TapeStation instrument (Agilent Technologies) and subjected to 52-bp paired-end sequencing.