RNA-seq of T. ulleungensis: Needle and Stem
RNA was extracted from the leaves using the Qiagen RNeasy Plant Mini Kit following the manufacturer's instructions. RNA quality was assessed using a TapeStation Automated Electrophoresis system 2200 (Agilent Technologies) and only samples with an RNA integrity number (RIN) greater than 8.0 were used for library preparation. Library preparation and sequencing were performed by Novogene using standard protocols. Sequencing was performed using Illumina NovaSeq 6000 generating paired-end reads of 100-150 bp in length.