GSM7746297: E13.5 eyecups, DKO (Six3F/F;Six6-/-;a-Cre), scRNAseq; Mus musculus; RNA-Seq
We followed instructions provided by 10x Chromium. Briefly, micro-dissected eye cups were dissociated into single cells using activated Papain (Worthington Biochemical) and then harvested in DPBS containing 0.04% BSA for cell capture using the 10x Chromium fluid device, targeting 10,000 cells for each sample. 10x Genomics Single Cell 3' kit (version 3)