GSM7467104: WT:APOE4-KI, biol rep 3; Mus musculus; RNA-Seq
Alexa 405+GFP+ phagocytic microglia were injected into the cortex and hippocampus (x = +1.5 mm, y = 2 mm, z = -1 and -2 mm; 2 ml per injection site) of WT mice. After 16 hours, astrocytes were isolated from the brain regions restricted to the injection sites. 1,000 isolated ACSA-2+/O1-/CD11b-/Cd45-/Olig4- astrocytes were lysed in 5 ul TCL buffer +1% beta-mercaptoethanol. RNA libraries were prepared for sequencing using standard Illumina protocols