GSM7835448: D42-WP3-CTR-NT_S2; Homo sapiens; RNA-Seq
RNA was extracted using RNeasy Mini Kit (QIAGEN).After RNA extraction, samples were treated with TURBO DNA-free™ DNase Kit (ambion), with 20 minutes DNase digestion according to manufacturer's instructions. Libraries were prepared starting from 10-100 ng of total RNA, using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina with the NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat) module (NEB). Protocol was performed according to manufacturer's instructions RNA-seq, a NextSeq™ 500 platform (Illumina) with the NextSeq 500/550 High Output Kit v2.5 (75 Cycles) kit (Illumina)