GSM7174006: H1_hESC_teratoma_H4K20me1_ChIP_rep1; Homo sapiens; ChIP-Seq
Teratoma tissue was rapidly frozen utilizing liquid nitrogen, and a section was excised using a razor blade. The tissue was then pulverized with liquid nitrogen using a mortar and pestle. The resulting crushed pellet was subsequently crosslinked with formaldehyde sonicated. Sonicated cell extracts were used for ChIP assays. ChIP-enriched DNA was end-repaired using the End-It DNA End-Repair kit (Epicentre), followed by addition of a single A nucleotide, and ligation of adapters. PCR was performed using Phusion High Fidelity PCR master mix