ATAC-seq for patient A27, sample F007, isolated microglia ex vivo
Isolated microglia were lysed with IGEPAL on ice, resuspended in transposase reaction mix and incubated at 37 degC. DNA was purified with Zymo ChIP DNA concentrator columns and amplified by PCR for 8-12 cyucles, gel size-selected for 160-290 bp, and sequenced.