GSM8079985: WT18SWTIPm1; Homo sapiens; OTHER
Two days after transfection, cells were harvested in lysis buffer A (20 mM Tris-Cl [pH 7.5], 100 mM NH4Cl, 10 mM MgCl2, 0.1% Tween20 supplemented with 1X cOmplete™ Mini Protease Inhibitor Cocktail [Roche, 11836170001]). Footprints were size-selected through electrophoresis, ligated to a 3'adpater, reverse transcribed. cDNA was circularized. Libraries were then prepared by PCR amplification.