RNA-Seq of PHS: adult liver
Total RNA was extracted from the tissue samples using the Qiagen RNeasy Mini Kit following the manufacturer's protocol. RNA quality and quantity were assessed using an Agilent 2100 Bioanalyzer and Qubit fluorometer, respectively. rRNA was removed using the Ribo-Zero rRNA Removal Kit to enrich for mRNA. The remaining RNA was fragmented and reverse-transcribed into cDNA using random hexamer primers. cDNA libraries were constructed using the Illumina TruSeq RNA Library Prep Kit according to the manufacturer's instructions. Libraries were indexed, pooled, and sequenced on an Illumina HiSeq 2000 platform to generate 2 x 100 bp paired-end reads.