GSM7469166: Aorta, WT, scRNAseq; Mus musculus; RNA-Seq
ProTracer mice were treated with tamoxifen at 8 weeks old. Then 8 weeks later, aortas were harvested from the mice, perfused with cold PBS, and then finely minced and transferred to the digestion mix (HBSS, 1U/µl Collagenase type 1 (LS004197, Worthington)). Tissues were incubated with the digestion mix at 37℃ for 30 minutes. The cell suspension was filtered through a 70 µm cell strainer and then pelleted by centrifugation at 300g for 5 minutes. After washing cells with 500ml HBSS, cells were loaded onto the 10x Chromium controller the generate Gel Bead-in-Emulsions (GEMs), which were further processed into single cell 5' gene expression libraries using Chromium Single Cell 5' Library Kit (v1.1). The cell suspense was loaded in 10x Chromium controller to generate GEMs, which were further processed into single cell 5ʹ gene expression libraries using Chromium Single Cell 5ʹ Library Kit (v1.1).