Post-transcriptional regulation of sandalwood
Approximately, 1g of the RNA was used to construct the library using NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, MA, USA) according to the manufacturers protocol. The ready to run final library was quantified using Qubit 3.0 fluorometer (ThermoFisher Scientific, USA) using DNA HS assay kit (ThermoFisher Scientific, USA) following manufacturers protocol. The insert size of the library was determined on 2100 Bioanalyser (Agilent Technologies, USA) following manufacturers protocol and paired-end sequencing was performed on Illumina HiSeq 2500 (Illumina, San Diego, CA, USA) with read length of 150 X 2 bp.