GSM8477018: Plscr5-/- P3 cochlear,rep1; Mus musculus; RNA-Seq
Total RNA of cochleae from P3 wildtype and Pou4f3(Δ/+) mice was extracted using RNAiso plus kit (TaKaRa, Japan). Total RNA of each sample was quantified and qualified by Agilent 2100 Bioanalyzer (Agilent Technologies, CA, USA), NanoDrop (Thermo Fisher Scientific, USA) and 1% agarose gel. 1 μg total RNA with RIN value above 6.5 was used for following library preparation. RNA libraries were prepared for sequencing using standard Illumina protocols