GSM8080212: ChIP-H3K27ac, shCHD9, rep1; Homo sapiens; ChIP-Seq
ChIP assays were conducted on 22RV1 cells, both with and without CHD9 knockdown. In brief, 5 million cells underwent cross-linking with 1% formaldehyde for 10 minutes at room temperature, followed by quenching with 125 mM glycine. Subsequently, the cell pellets were twice washed with cold PBS and resuspended in 300 μL of modified RIPA buffer (10 mM Tris-HCL, pH=8; 1 mM EDTA; 140 mM NaCl; 1% Triton X-100; 0.1% SDS; 0.1% sodium deoxycholate) supplemented with protease inhibitor. The resuspended pellet underwent sonication for 18 cycles (30s on, 30s off) at 4 °C using  Bioruptor (Diagenode). The purified DNA was then subjected to Illumina ChIP-Seq library construction using the ThruPLEX DNA-seq kit (Takara, R400428).