GSM8266789: pESC-2; Sus scrofa; RNA-Seq
Total RNAs were extracted by TRIZOL. In brief, 1 μg total RNAs were used, library strategy are followed by mRNA-seq V3 Library Prep Kit for Illumina (Vazyme, NR611), library DNA was purified by VAHTS DNA Clean Beads (VAHTS, #NR411). High- throughput sequencing was performed using Illumina HiSeq Xten (Illumina) platform with PE150.