RNAseq of THP-1 exposed to P. gingivalis
Total RNA was extracted from the sample and enriched for mRNA using poly-A selection or ribosomal RNA depletion. The enriched mRNA was fragmented, and cDNA synthesis was performed using random primers, incorporating dUTP during second-strand synthesis to maintain strand specificity. The double-stranded cDNA was then end-repaired, A-tailed, ligated with sequencing adapters, amplified by PCR, and assessed for quality before sequencing on the DNBSEQ platform.