GSM6888291: TetHighProg, high avidity GSW11-specific CD8+ TILs from progressing tumors [TetHigh_2]; Mus musculus; RNA-Seq
RNA was harvested using Zymo Quick RNA Miniprep Kit (Zymo, USA) prior to cDNA synthesis. The purified RNA was quantified using Qubit 4 Fluorometer (Invitrogen) and the quality was determined using the Agilent 2100 Bioanalyzer with the Agilent RNA 6000 nano kit (Agilent Technologies). RNA was reverse transcribed using SuperScript II (Invitrogen) and cDNA pre-amplification was performed using KAPA HiFi HotStart ReadyMix (Roche Molecular Systems) for 22 cycles of amplification. The cDNA libraries were purified with Agencourt AMPure XP beads (Beckman Coulter) according to the manufacturer's instructions. The cDNA product was then analyzed using the Agilent 2100 Bioanalyzer with the Agilent High Sensitivity DNA kit (Agilent Technologies). Tagmentation and library preparation was undertaken using the Illumina Nextera XT DNA Library Preparation Kit (Illumina) according to manufacturer's instructions.