RADseq of Cladonia sandstedei: isolate 18574
DNA was submitted to the University of Wisconsin-Madison Biotechnology Center for library preparation and sequencing. DNA concentration was verified using the Quant-iT PicoGreen dsDNA kit (Life Technologies, Grand Island, NY). Libraries were prepared as in Elshire et al. (2011) with minimal modification; in short, 50 ng of DNA was digested using the 5-bp cutter ApeKI (New England Biolabs, Ipswich, MA) after which barcoded adapters amenable to Illumina sequencing were added by ligation with T4 ligase (New England Biolabs, Ipswich, MA). Adapter ligation proceeded in batches of 96 samples which were then pooled and amplified to provide library quantities amenable for sequencing. Adapter dimers were removed by SPRI bead purification. The quality and quantity of the finished libraries were assessed using the Agilent Bioanalyzer High Sensitivity Chip (Agilent Technologies, Inc., Santa Clara, CA) and Qubit dsDNA HS Assay Kit (Life Technologies, Grand Island, NY), respectively.