GSM8499451: LCL, YY1, rep1; Homo sapiens; OTHER
25 million cells per condition were fixed with 1% formaldehyde for 10 min and then quenched with 0.125 M glycine for 5 min at RT. Ligation products were immunoprecipitated with 50 µg YY1 antibody. 5 μL of Streptavidin C-1 beads were used for biotin pull-down After the last wash with Tween Wash Buffer bead-bound DNA was used to prepare libraries with Ultralow Library Systems V2