GSM8340240: Cutaneous melanocytes; Danio rerio; OTHER
Respective tissues were mechanically dissociated, digested in TrypLE for 45 min (Invitrogen, 12563011), 40uM filtered, spun down, and resuspended in FACs buffer (dPBS Mg+/Ca+ free, with 2% FBS, pen/strep). Cells were heat-lysed at 65°C followed by cDNA synthesis with barcodes. All the barcoded material from one plate was pooled into one library and amplified using in vitro transcription. Following amplification, library preparation was done following the CEL-Seq2 protocol to prepare a cDNA library for sequencing using TruSeq small RNA primers (Illumina).