16S bacteria: stigma
DNA extraction and PCR amplification were carried out by A&A Biotechnology (Poland), while Macrogen (Netherlands) handled NGS library preparation and performed Illumina and Sanger sequencing for the 16S rRNA and ITS products. The microbial populations inhabiting the studied samples were investigated by sequencing the V3-V4 region of the 16S rRNA gene and the ITS region.