single-cell RNAseq of 3D cocultures : Healthy skin and systemic sclerosis B-cells
Single-cell library preparation was realized using Chromium Next GEM Single Cell 5' Kit v2 and Chromium Next GEM Chip K Single Cell Kit (10X Genomics, Pleasanton, CA, USA) as per the manufacturers instructions. Sequencing was performed on a NovaSeq 6000 sequencing system (Illumina, San Diego, CA, USA) using a 100bp chemistry (paired-end mode).