RNAseq of subcutaneous adipose tissue macrophage populations, AT-macrophages derived from 3D cultures and BM-cultured macrophages
RNAseq of subcutaneous adipose tissue macrophage populations, AT-macrophages derived from 3D cultures and BM-cultured macrophages
scAT macrophages were sorted based on CD45+, F4/80+ and CD11b+. AT-macrophages derived from 3D cultures and BM- cultured macrophages were harvested after 13 days in culture.    RNA were extracted from sorted sc-AT macrophages, AT and BM cultured macrophages.  and purified using a Promega extraction kit (Relia PreP ™ RNA Cell Miniprep).  cDNA libraries were generated according to Illumina Stranded mRNA Prep kit (Illumina) from 100ng tRNA. cDNA was amplified for 13 cycles and barcoded with IDT® for Illumina® RNA UD Indexes Set A, Ligation