RNA-Seq of patients with ESCC
RNA from 24 tissue samples was extracted using RNeasy Mini Kit and were quantified using Qubit RNA assay HS. Total RNA sequencing libraries were prepared using NEB Ultra RNA-Seq Library preparation kit. Further the prepared libraries were quantified using Qubit High sensitivity Assay and the obtained libraries were pooled and diluted to final optimal loading concentration before cluster amplification on Illumina flow cell. Illumina HiSeq 4000 instrument was further used to generate 60M, 100bp paired end reads