GSM7116816: A375 cells, biol rep 2; Homo sapiens; RNA-Seq
RNA was extracted from cells or tissues using TRIZOL (Thermo Fisher Scientific) following manufacturer's instructions. DNase treatment was performed for 30 minutes at 37oC and DNase was removed via phenol-chloroform extraction. rRNA-depleted RNA was used to generate the RNA-sequencing libraries using NEB Ultra II directional kit (New England Biolabs). RNA sequencing libraries were prepared using TruSeq Stranded mRNA Library Prep Kit following manufacturer's protocol.