Genome sequence of Candidatus Saccharibacteria TM7_HMT488_106.1.1
Bacteria were grown in co-culture with Arachnia propionica 10397 in Brain Heart Infusion medium and separated by filtration through a 0.2 micron filter. Cells were concentrated using a 100 kDa protein concentrator and DNA was extracted with the Zymo Research Quick-DNA Fungal/Bacterial Miniprep Kit. Extracted DNA was sent for sequencing at MicrobesNG, Birmingham, UK, where long read genomic DNA libraries were prepared with Oxford Nanopore SQK-LSK109 kit with Native Barcoding EXP-NBD104/114 (ONT, United Kingdom) using 400-500 ng of HMW DNA.