Identification of m6A methylation-related genes in cerebral ischemia-reperfusion of Breviscapus therapy based on bioinformatics method
Identification of m6A methylation-related genes in cerebral ischemia-reperfusion of Breviscapus therapy based on bioinformatics method
Primary cortical neuron culture was established from C57BL/6 neonatal mice aged 1-3 days by collecting brain tissue from the cerebral cortex after decapitation, and divided into control group, OGD/R group, and breviscapine administration group. Total RNA was isolated and purified by TRIzol reagent following the instruction manual. These compounds doublets of DNA and RNA were converted into DNA doublets, dUTP Solution was incorporated into the doublets at the same time, and the ends of the double-stranded DNA were complemented to flat ends. An additional base was added to each end to enable ligation with a linker with a T base at the end, and its fragment size was screened and purified using magnetic beads. The second strand was digested with UDG enzyme, and then PCR was performed to form a library with a fragment size of 300bp Â± 50bp.