GSM8219064: Placenta_fetus#7; Sus scrofa; RNA-Seq
Total RNA was isolated from the male and female placenta (in triplicates) using an AllPrep DNA/RNA Mini Kit (Qiagen, Cat No./ID: 80204). A Nanodrop 1000 spectrophotometer (Thermo Fisher Scientific) was used to measure RNA concentration of RNA. The RNA Integrating Number (RIN) was determined using Agilent 2100 bioanalyzer. RNA was supplied to the Novogene Cooperation Inc (Sacramento, CA, USA) for preparation of sequencing libraries and sequencing (RNA-seq). Each library was sequenced to 20 million paired end reads of 150 bases using a NovaSeq 6000 sequencer.