Post-transcriptional regulation of sandalwood
Library construction was done using TruSeq Small RNA Library Preparation Kit (Illumina Inc., San Diego, CA, USA). Prior to sequencing, adaptor ligation, reverse transcription, PCR enrichment, purification and size selection of the cDNA library was carried out following manufacturers protocol. The quality control of the library was carried out in three steps: preliminary library concentration check using Qubit 2.0 Fluorometer (ThermoFisher Scientific, USA), insert size assessment using 2100 Bioanalyzer Instrument (Agilent Technologies, USA) and quantitative PCR to estimate the library concentration. Single-end sequencing was carried out on Illumina platform HiSeq 2500 (Illumina, San Diego, CA, USA) with read length of 50 X 1 bp.