GSM7889971: C2C12 WT D5 Sample 2; Mus musculus; RNA-Seq
Total RNA was isolated from n=3 cultured C2C12 myoblasts at D0 and D5 using Tri-Reagent according to the manufacturer's instructions (Sigma Aldrich). RNA quality was accessed using a 2100 Bioanalyzer System (Agilent), and samples with RNA Integrity Number (RIN) greater than 8.0 were sequenced. Illumina RNA-Seq was performed by the Genomics Services Laboratory (GSL) at Nationwide Children's Hospital (Columbus, OH). Quality assessment of the RNA-Seq data was performed using NGS-QC-Toolkit (v 2.3.3) with default settings (Patel & Jain 2012). Quality filtered reads were aligned to the mouse reference genome GRCm38 (mm10) using the Tophat2 (v 2.0.0) aligner with default settings (Kim et al. 2013).