GSM7117053: 5 Beta_Week8; Mus musculus; RNA-Seq
High-quality total RNA (>200nt) was extracted using standard Trizol reagent (Invitrogen) according to manufacturer instructions and the resultant aqueous phase was mixed (1:1) with 70% RNA-free ethanol and added to Qiagen Rneasy mini kit columns (Qiagen) and the kit protocol was followed with DNA digestion on column step performed. RNA libraries for RNA-seq were prepared Takara Pico-Input Strand-Specific Total RNA-seq for Illumina v2 Kit following manufacturer's protocols.