RNA-seq of EndoC_BH1 cells
RNA-seq of EndoC_BH1 cells
7 million cells for each of three biological replicates were collected off plates using trypsin and stored in TRIzol at -80 C until RNA extraction.  Total RNA was extracted from TRIzol (Invitrogen) following manufacturer protocol. Any DNA contamination was removed from the extracted total RNA using the RNase Free DNase (Promega) followed by column clean-up with RNeasy Mini Kit (Qiagen). Depletion of rRNA and RNA-seq libraries were prepared from 1ug of total RNA using the SMARTer Stranded Total RNA Sample Prep Kit (Takara Bio USA, Inc.) following standard protocols.