GSM7266496: Kidney OSOM, MCPD, Day90; Rattus norvegicus; RNA-Seq
Total RNA was purified with an AllPrep DNA/RNA Mini Kit (Qiagen) following the manufacturer's protocol. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads, and then the mRNA was fragmented into small pieces. The first-strand cDNA was synthesized using random hexamer primers, followed by synthesis of the second-strand cDNA using dTTP for the non-directional library. These cDNA fragments underwent an end repair process, adding a single “A” base and then ligating the adapters. These products were purified and enriched by PCR to create the final cDNA library.