Hi-C genome sequencing of Cymbidium tracyanum
C. tracyanum cells were treated with formaldehyde to fix the cross-linked complexes, and then the cross-linked DNA was cut with the restriction enzyme MboI. The sticky ends were labelled with biotin and ligated to form a circular chimeric molecule, and the was then purified and fragmented DNA were selected for library construction. Four libraries were sequence on Illumina Hiseq X ten and generated 544 Gb of paired-end reads.