Genome sequence of Candidatus Saccharibacteria TM7_HMT488_106.1.1
Bacteria were grown in co-culture with Arachnia propionica 10397 in Brain Heart Infusion medium and separated by filtration through a 0.2 micron filter. Cells were concentrated using a 100 kDa protein concentrator and DNA was extracted with the Zymo Research Quick-DNA Fungal/Bacterial Miniprep Kit. Extracted DNA was sent for sequencing at MicrobesNG, Birmingham, UK, where genomic DNA libraries were prepared using the Nextera XT Library Prep Kit (Illumina) following the manufacturers protocol with the following modifications: input DNA is increased 2-fold, and PCR elongation time is increased to 45 seconds.