Illumina NovaSeq 6000 paired end sequencing of SAMD00653793
The libraries were prepared by Novogen using the following procedure (https://www.novogene.com/). The genomic DNA is randomly fragmented to size of 350bp, and then DNA fragments were end polished, A-tailed, and ligated with the adapters of Illumina sequencing, and further PCR enriched with primers of P5 and P7 oligos. The PCR products as final construct of the libraries were purified (AMPure XP system), followed by quality control test that included size distribution by Agilent 2100 Bioanalyzer (Agilent Technologies, CA, USA), and molarity measurement using real-time PCR.