GSM7835554: Premorbid rat, T cells, HLA B27, Replicate 6; Rattus norvegicus; RNA-Seq
Total RNA was purified from mLN Tn sorted from 4 wk- (Monarch® Total RNA Miniprep Kit, NEB) or 3 mo-old rats (RNeasy micro kit, QIAGEN). Samples with RNA integrity number >7 were sequenced. Libraries were prepared using NEBNext® Low Input RNA Library Prep Kit (3 mo-old) or NEBNext® rRNA Depletion Kit and NEBNext® Ultra II Directional RN Library Prep Kit (4 wk-old) and sequenced on an Illumina HiSeq 2000 (3 mo-old) or NextSeq 500/550 (4 wk-old), paired-end 75 bases.