GSM8476913: High Light, -10 h (night), rep 1; Chlamydomonas reinhardtii; RNA-Seq
Cell pellets were placed in lysis buffer (50 mM Tris-Cl, 200 mM NaCl, 20 mM EDTA, 2% SDS, 1 mg/mL proteinase K), flash frozen in LN2, and stored at –80°C prior to purification. Total RNA was purified in batches using standard protocols that accompany commerial kits including: TRIzol (Invitrogen), MaXtract HD columns (QIAgen) and miRNeasy Mini  kits (QIAgen). An additional EtOH precipitation was required to remove contaminants from the kit (as judged by the NanoDrop A260/A230 ratio). standard Illlumina HiSeq compatible libraries