GSM8532383: RNAseq, Organoid, day4, WT, rep2; Mus musculus; RNA-Seq
Liver tissue and Hepa1-6 cells were lysed in TRIzol and RNA isolated with Quick-RNA Miniprep Kit (Zymo, R1055). RNA from organoid samples was extracted using the single cell RNA isolation Kit (Norgen Biotek). For liver tissue samples libraries were prepared using TruSeq Stranded Total RNA Library Prep Gold (Illumina, 20020599). For Hepa1-6 cells and organoid  day 3-8 samples, libraries were prepared using the Stranded mRNA Prep, Ligation kit (Illumina, 20040534). For day 15 samples were NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB, E6420).