GSM8443995: MEFs treated with doxycycline, Day 1, biol rep 1, RNA-seq; Mus musculus; RNA-Seq
MEFs and cells undergoing reprogramming for 1, 2, 3, 4, 6 and 8 days were lysed and their RNA was extracted using NucleoZOL (740404, Macherey-Nagel), as by the manufacturer's instructions. The RNAs were DNAseI-treated and cleaned-up using the classic phenol-chloroform procedure. Two biological replicates were prepared for each time-point. RNA-seq libraries were prepared using the NEBNext® Ultra II™ Directional RNA Library prep Kit for Illumina® (E7760, NEB) with 1 ug of total RNA input, according to the manufacturer's guidelines.