GSM8782609: PC Scar; Mus musculus; RNA-Seq
PC cells with constitutive Cas9 expression were transfected with a barcode library using Lipofectamine 3000, expanded for three passages, sorted via flow cytometry to isolate RFP-positive live cells (~1 million sorted, ~20,000 recovered), and processed for scRNA-seq. DNA libraries were generated for gene expression (10× Genomics 3' v2 kit), barcode, and scar regions, with barcodes amplified using root and nested PCR, purified via AMPure XP beads, indexed with dual-index primers, and sequenced on Illumina NovaSeq, followed by mapping with CellRanger.