GSM8078767: WT replicate 2 in G2; Homo sapiens; OTHER
Cells were trypsinized and washed once with PBS, followed by fixation with formaldehyde for MicroC. Micro-C experiments in G1- or G2-arrested WT and TOP2DKO HCT116 cells were performed in collaboration with Dovetail Genomics as previously described (Zhang et al., 2023). Briefly, at least 2 libraries per condition/genotype were generated using ~1 million cells and pooled together for shallow sequencing. Those libraries that passed standard QC criteria (i.e., long-/short-range interaction ratio, PCR duplication levels) were pooled and paired-end sequenced on a NovaSeq6000 platform (Illumina) to >600 million read pairs per replicate