tumor, obese Trem2-/-, replicate 2, scRNAseq  mRNA
Library construction was performed according to the manufacturer's instructions for the Chromium Next GEM Single Cell 5' HT v2 kit from 10X Genomics. In brief, the CD45+ cells were resuspended in the Master Mix and loaded in conjunction with partitioning oil and gel beads into the Chromium Next GEM Chip N to generate the gel bead-in-emulsion (GEM). Once GEMs were generated, the gel beads dissolved and the copartitioned cell was lysed. Reverse transcription then generated 10X barcoded cDNA from the poly-adenylated mRNA. The cDNA was purified using Silane Dynabeads and amplified to generate sufficient material to construct libraries. The amplified full-length cDNA was used to enrich full-length V(D)J segments by PCR amplification with primers specific to the TCR. Enzymatic fragmentation and size selection were used to optimize for both 5' gene expression library construction and V(D)J library construction. Finally, P5, P7, i5 and i7 sample indexes and Illumina R2 sequence were added to the cDNA via End Repair, A-tailing, Adaptor Ligation, and Sample Index PCR.