GSM7925536: haf2_ko_air_H3K14ac_rep1; Arabidopsis thaliana; ChIP-Seq
Three-day-old etiolated haf2_ko seedlings treated with air or 4h ethylene were harvested and crosslinked in 1% formaldehyde, and the chromatin was isolated and sheared by Bioruptor. Anti-H3K14ac (Millipore; 07-353) and anti-H3K23ac (Millipore; 07-355) antibodies together with Dynabeads were added to the sonicated chromatin followed by incubation overnight to precipitate histone bound DNA fragments. Chromatin-immunoprecipitated DNA after elution was prepared for library construction by using NEBNext® Ultra™ II DNA Library Prep with Sample Purification Beads according to standard operating procedures and then sequenced using Illumina NovaSeq X Plus.