GSM8443475: PRO-seq_Rep2_HS30min; Canis lupus familiaris; OTHER
Chromatin was used as starting material, after which Precision Run-On sequencing (PRO-seq) was performed as previously described in Mahat et al. (2016), Nat. Protocol 11, 1455–1476, using sample-specific in-line barcodes described in Vihervaara et al. (2023), Nat. Commun 14, 7039. Libraries were constructed using TruSeq small-RNA adaptors as described in VIhervaara et al. (2023), Nat. Commun 14, 7039.