GSM8560982: SM512_RNA_SEQ_genes; Mus musculus; RNA-Seq
All prostate and tumor tissues were microdissected at necropsy, flash frozen in liquid nitrogen, and stored at -80°C Total RNA was purified from TRAMP tumor samples that were also evaluated through hMeDIP-seq as there was sufficient tissue for both DNA and RNA extraction from the tumors. To obtain sufficient total RNA for normal mouse prostate RNA-seq analysis, only RNA or DNA could be extracted from a single normal mouse prostate due to their small size. All total RNAs were extracted using TRI Reagent according to the manufacturer's instructions. Purity and concentration were assessed by measurement of the A260/280 ratios using a Nanodrop intrument and potential degradation was assessed by analysis with Advanced Analytical Technical Instruments Fragment Analyzer. Libraries were developed using TruSeq V2 RNA sequencing library kit from Illumina following recommended procedures. Libraries were sequenced with the Illumina NextSeq 500 Sequencer.