GSM8172940: Day 2 Vehicle; Mus musculus; RNA-Seq
Pancreas was removed, placed on ice and dissociated using a GentleMACS for 60 seconds, tissue was strained, debris removed using Miltenyi Debris Removal solution per manufacture's protocol. Cells were underlaid with Lympholyte-M and seperated per manufacture's protocol. CD45+ CD3+ TcR-beta+ cells were isolated by flow sorting on a Sony MA-900. Cells were frozen in 90% FBS, 10% DMSO and then all samples were subjected to RNA isolation at the same time using RNAeasy Kit (Qiagen) using manufacture's protocol. Libraries were produced using the manufacture's protocol (single cell, 3', version 2, 10x Genomics) after passing quality control using the Agilent 2100 Bioanalyzer.