GSM8266724: melb-a cells, lenti- shLuciferase biol rep3; Mus musculus; RNA-Seq
RNA was extracted from cells using TRIzol and Direct-zol RNA MiniPrep Plus kit (Zymo R2072). PolyA RNA was isolated using the NEBNext® Poly(A) mRNA Magnetic Isolation Module and barcoded libraries were made using the NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich MA).