GSM8746899: RBEC_OGDR_6; Rattus norvegicus; RNA-Seq
Total RNA was extracted by column based extraction kit (Zymo) RNA-Seq libraries (NEBNext Ultra II Directional) were prepared according to the manual of the manufacturers. Summarized, enriched poly-Adenylated mRNA is fragmented, followed by cDNA synthesis using random hexamers. The cDNA fragments undergo end repair, A-tailing followed by ligation of Illumina P5 and P7 adapters. After PCR amplification using a minimum number of cycles, purification by SPRI beads, the quality of the libraries was controlled for size-distribution and quantity. Sequencing was performed on an Illumina NEXTSEQ 500 instrument with 1x75 bps.