GSM8499049: HEK293-FRT-TRIM25KO TRIM25-FLAG (M9) add-back, iCLIP immunoprecipiated for TRIM25, SINV infected (9h), rep2; Homo sapiens; OTHER
Cells were washed with PBS 1x, and UV irradiated with 300 mJ/cm2 at 254 nm. Cells were then lysed in 1 ml RIPA buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1% Triton X-100, 0.1% SDS, 0.5% wt/vol Na deoxycholate and 0.2 mM AEBSF). Libraries were constructed using iCLIP2 procotol (https://doi.org/10.1016/j.ymeth.2019.10.003)