GSM6998092: LS_124_HAM_24h -Mtb; Homo sapiens; RNA-Seq
Cells were retrieved from plates using 5mM EDTA (Sigma) and incubating the cells on ice for 30mins. Cells were stored in RNALater (Invitrogen) at -80C. Prior to shipment the cells were spun down and RNALater replaced with Trizol. RNA was isolated using Quick-RNA Microprep Kit (R1050; Zymo Research) as per the manufacturer's instructions. Isolated RNA was quantified using the Qubit RNA High Sensitivity Assay Kit (Q32852) and Qubit 4 Fluorometer (both from ThermoFisher Scientific). RNA sequencing libraries were prepared from 25-500ng of Total RNA using the NEB Next directional RNA library preparation Kit with poly (A) enrichment module (Ipswich, MA). stranded RNA-seq