GSM8405615: Male healthy donors, monocytes, rep3; Homo sapiens; RNA-Seq
Total RNA was purified using RNeasy Micro Kit (QIAGEN). DNase treatment was performed using RNase-free DNAse Set (QIAGEN), according to the manufacturer's instructions. RNA was quantified with Qubit 2.0 Fluorometer (Thermo Fisher) and its quality assessed by a 2100 Agilent Bioanalyser (Agilent Technologies). Minimum quality was defined as RNA integrity number (RIN) > 8. 300 ng of total RNA were used for library preparation with TruSeq Stranded mRNA (Illumina)