GSM8267039: LCM, Latently infected cells with uninfected bystanders, HTO; Homo sapiens; RNA-Seq
The sorted cells were counted after trypan blue staining for estimating the proportion of viable cells and introduced into the 10X Genomics Chromium platform workflow for high throughput scRNAseq library preparation (V2 3' GEX assay). For sequencing, batched libraries were run on an Illumina NovaSeq S4 flowcell (2 x 100 nt paired-end read length) with a target of 50,000-100,000 reads per cell. FACS sorting followed by Genomics Chromium Next GEM Single Cell 3' Reagent Kits v.3.1 (CG000204 Rev D) protocol