GSM8075932: SOD1-ALS iPSC-derived miRNA MN_2; Homo sapiens; miRNA-Seq
Total RNA was isolated using miRNeasy Mini Kit (Qiagen) according to the manufacturer's protocol for small amounts of RNA for the neuronal fraction including a column DNase digest for all samples. 1 ug of total RNA was used to construct sequencing libraries using the TruSeq Small RNA Library Prep Kit (HiSeq 2500, Illumina) following manufacturer's protocols.