GSM8080398: Control, 30 min, rep 4; Arabidopsis thaliana; RNA-Seq
Frozen material was thoroughly grinded on liquid nitrogen and total RNA was extracted by means of the RNeasy plant mini kit (Qiagen), and a DNase treatment (Qiagen) following manufacturer´s instructions. RNA libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina according to manufacturer's instructions. Libraries were sequenced on an Illumina platform generating paired-end reads fragments of 150 bp.