16S amplicons of dust from car cabin filters
~20g was cut out of each filter and homogenized for 1 min in a 100 ml saline solution of 0.05 tween20 with a paddle mixer. Next, a 50 ml aliquot of this solution was collected and differentially centrifuged for 3 min at 250 rpm. Then 3 ml aliquots of supernatant were spun at 10,000 rpm to form pellets for DNA extraction