GSM7888022: AR97Q-1; Mus musculus; RNA-Seq
Total RNA was extracted from the OL cell model of SBMA and control using the RNeasy Mini Kit (QIAGEN). The quality and quantity of the RNA obtained was checked using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, United States), and analyzed with the Agilent 2100 Bioanalyzer system (Agilent Technologies Inc). A library was independently prepared with 1ug of total RNA for each sample by Illumina TruSeq Stranded mRNA Sample Prep Kit (Illumina, Inc.)