GSM8619554: BGLT3 KD odd rep2; Homo sapiens; OTHER
About 2×107 K562 cells were crosslinked by 1% glutaraldehyde followed by sonication. Lysates were clarified from sonicated chromatin and RNA-DNA complexes were isolated by antisense biotinylated oligos on streptavidin beads according to the protocol published by Chu et al., 2011. Then the DNA was purified by PhOH:Chloroform: Isoamyl for subsquential processes. Libraries were prepared according to NEB's instructions accompanying the NEBNext Ultra II DNA Library Prep Kit (E7103S) and NEBNext Multiplex Oligos for Illumina (E7335S).