GSM7920061: Hippocampal_Sample23 [H100]; Ovis aries; RNA-Seq
RNA was extracted from snap-frozen hippocampal and lung tissue using RNeasy Plus Mini Kit (QIAGEN, Hilden, Germany) according to the manufacturer's instructions. The concentration of extracted RNA and RNA Integrity Number (RIN) was determined using Agilent Technologies RNA Nano Chip as per manufacturer's instructions. All RNA extracts were diluted in nuclease-free water to yield a final RNA concentration of 30 ng/μl. Hippocampal and lung tissue bulk RNA sequencing was performed by Novogene Singapore. NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (Cat No. 7420)