Trex1 knock out in cancer cell lines
Trex1 knock out in cancer cell lines
Cultured cell lines were spun into cell pellets were snap frozen. Snap frozen cell pellets from cultured cell lines were directly lysed with RLT lysis buffer of the Rneasy Plus Mini kit (Qiagen).  Total RNA was quantified with the Qubit RNA HS Assay Kit (Thermo Fisher Scientific) and quality was assessed using RNA ScreenTape on a TapeStation 4200 (Agilent Technologies). For sequencing library generation, the TruseqStranded mRNA kit (Illumina) was used with an input of 100-1000 nanograms of total RNA. Libraries were quantified with Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific) and the average library size was determined using D1000 ScreenTape on TapeStation 4200 (Agilent Technologies).