GSM7116538: Liver, DIO, exposure, rep2; Mus musculus; RNA-Seq
The frozen tissues of lung and liver (approximately 5mg) were subjected to RNA isolation using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). Oligo dT magnetic beads were employed to trap mRNAs with poly A tails, and the mRNAs were fragmen- ted and reversely transcribed to double-stranded cDNA (dscDNA) by random primers. The cDNAs were ligated adaptors and subjected to amplification by PCR. The PCR products were then denatured, and single stranded PCR products were cyclized by splint oligos with DNA ligase to construct cDNA library.