RNASeq of Lygodium microphyllum: pooled gametophytes at -20C
Tissues for transcriptome sequencing were flash frozen in liquid nitrogen and stored at -80C until extraction. RNA was extracted from sterile leaf, fertile leaf, elongating leaf, rhizome, rachis, and fiddleheads using a modified CTAB extraction (Pelosi et al. 2024), purification with the Spectrum Plant Total RNA Kit or Zymo RNA Clean and Concentrator and on-column DNAse treatment. mRNA Illumina libraries were constructed using this RNA and sequenced at NovoGene.