paired-end sequencing of C22 phage: purification using sucrose density gradient ultracentrifugation
The phage DNA was extracted using QIAprep Spin Miniprep kit (Qiagen, USA) with modified protocol. The library preparation was performed using the NextSeq 500/550 Kit v2.5 (paired-end, 300 cycles) and the sequencing was performed in NextSeq 550 Series (Illumina, USA).