Natural Killer Cells from High Grade Serous Ovarian Carcinoma ascites
Natural Killer Cells from High Grade Serous Ovarian Carcinoma ascites
First, frozen patient peritoneal cells were thawed and counted.  Next, Natural Killer (NK) cells were purified using the negative selection kit from Miltenyii (130-092-657), according to the manufactures protocol. In order to increase the purity, NK cells underwent a second round of purification using the positive selection kit from Miltenyii (130-050-401), according to the manufactures protocol. The purity was determined via FACS and microscopy and the NK cells were then lysed in 1 ml Trizol. RNA was isolated using Trizol/TriFast (30-2010), according to the manufacturers' protocol.  DNA was removed using the DNA-freeâ„¢ DNA Removal Kit (Ambion, AM1906), according to the manufacturers' protocol. Illumina Truseq mRNA stranded, according to manufacturers' instructions