GSM8498970: High light, 5 mM nitrate, rep 8; Arabidopsis thaliana; RNA-Seq
On the 30th day after transplanting, two leaf punches were taken from each individual plant and immediately flash-frozen for RNA-sequencing. Frozen samples were powdered in 2ml tubes with 8-12 beads of Matrix D (cat #116540434 MP biomedicals) with the FastPrep-24 cryo rotor using the following program (2x 5.5m/s2 x 60 sec). Kapa Hyper Stranded mRNA library kit (Roche)