GSM8078371: WT_CDM; Lactococcus lactis; RNA-Seq
RNA was harvested using Rneasy plus according to manufacturer indications. Samples were adjusted to 100 ng/µl and checked for integrity with an RNA Nano Chip (Agilent Technologies). RNA sequencing library preparation was prepared using NEBNext rRNA Depletion Kit (Bacteria)  and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina following manufacturer's instructions (NEB, Ipswich, MA, USA) The sequencing libraries were multiplexed and clustered on the flowcell. After clustering, the flowcell was loaded on the Illumina NovaSeq 6000 instrument according to manufacturer's instructions. The samples were sequenced using a 2x150 Pair-End (PE) configuration.