GSM6998037: Mesenchymal cells, PBS group, sample 2, pooled from 3 mice; Mus musculus; RNA-Seq
Mesenchymal cells and immune cells from the lungs were sorted based on their surface marker expressions. Total RNA extracted following the instructions of the 10X genomics kit Mesenchymal cells scRNA-seq libraries were prepared using the 5' single cell gene expression kit and immune cells scRNA-seq libraries were prepared using 3' CellMultiplex kit (10x Genomics) accoding to the manufacturer's instruction.