Comparative RNA-seq Analysis of Slc38a8 (SNAT8) knockout mouse: Evaluating the Impact of the gene knockout on biological pathways
Comparative RNA-seq Analysis of Slc38a8 (SNAT8) knockout mouse: Evaluating the Impact of the gene knockout on biological pathways
Samples were immersed in Davidson's fixative for 48 hours, after which they were transferred to 70% EtOH for between 1 to 3 days. They were then processed using an automatic vacuum tissue processor and subsequently embedded. mice are housed in specialized cages with controlled environments, fed a standard diet, and given filtered water, with conditions adjusted as per experiment requirements.  All materials and personnel undergo strict decontamination processes, and health monitoring of sentinel mice follows FELASA recommendations, ensuring adherence to Directive 2010/63/EU  RNA from the mouse retina layer was isolated using microdissection from FFPE samples. The extraction was conducted using the AllPrep DNA/RNA FFPE Kit (Qiagen CAT NO/80204). SMARTer Stranded RNA-Seq Kit RiboZero protocol