GSM8267209: Young,Uterus,rep1 [Y20_H3K4me3]; Mus musculus; ChIP-Seq
Histone ChIP was performed on pellets of 200,00 uterine stromal cells. Chromatin was digested with micrococcal nuclease and ChIP performed with antibodies against H3K4me3 (Abcam ab8580) and H3K9me3 (Abcam ab8898). 10% of chromatin was kept back and sequenced as Input. Following the chromatin immunoprecipitation procedure, DNA was isolated using proteinase K digestion followed by clean-up using the QiaQuick PCR purification kit (Qiagen 28104). Input and ChIP DNA was used for library build with the NEBNext Ultra II DNA library prep kit (Illumina E7645)