GSM8078682: WT replicate 1; Homo sapiens; OTHER
Cells were trypsinized and incubated in lysis buffer for CUT&RUN as described by the manufacturer of the CUT&RUN Assay Kit (Cell signaling #86652). Libraries were prepared using the SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® (Cell signaling #56795) following manufacturer's instructions. Indexed libraries were pooled at equimolar concentrations and sequenced in an illumina NextSeq550 in paired end mode (2x75bp)