GSM8080486: Heart left ventricle, excised under isoflurane anesthesia, kidney disease, 225 days, replicate 6; Mus musculus; RNA-Seq
Total RNA from tissue was isolated and purified using TRIzol (15596018, ThermoFisher Scientific) with tissue disruption by the TissueLyser II (Qiagen), Phasemaker tubes (A33248, ThermoFisher Scientific), and PureLink RNA Mini Kit (12183018a, ThermoFisher Scientific) with PureLink DNase (12185010, ThermoFisher Scientific) according to manufacturers' instructions. RNA quality was verified using the Bioanalyzer 2100 Expert (Agilent). All samples met minimum RNA Integrity Number of 8.0 KAPA RiboErase