GSM8078982: MIN6 stable cells overexpressing miR-146a-5p treated with proinflammatory cytokines - replicate1 [P65-OE-Cy]; Mus musculus; RNA-Seq
Total RNA was isolated using miRNeasy mini kit (217004; Qiagen). RNA concentration was measured using a NanoDrop 2000 (ThermoFisher Scientific). One hundred nanograms of total RNA was used for library preparation.Library amplification was performed following the KAPA mRNA Hyper Prep Kit Technical Data Sheet, KR1352 – v4.17 (Roche Corporate)