GSM8746535: N206_BSJ_DMSO_rep3; Homo sapiens; RNA-Seq
RNA was isolated as described in the 'RNA isolation and RT-qPCR' section. RNA quality was measured using the Fragment analyzer 12 capillary array prior to profiling A total of 55ng of RNA was used as input for the library preparation with the Illumina Stranded mRNA prep (#20040534). During the library preparation 15 PCR cycles were used. qPCR quantification of the libraries was done using the Kapa Library Quantification Kit for the Illumina LightCycler 480 (Roche diagnostics #07960298001). RNA-Seq libraries were sequenced on the Illumina NextSeq 2000 platform using the NextSeq 2000 P2 Reagents 100 Cycles single-end kit (Illumina #20100987).