Single-cell DNA nanopore sequencing of Scytonema sp. PRP1
DNA of Scytonema sp. PRP1 was extracted via single-cell MALBAC library amplification of human neurons. Subsequently, PCR was utilized to amplify the DNA, enabling the generation of sufficient material for sequencing. Utilizing Porechop, adapters were then trimmed from Oxford Nanopore reads, including MALBAC and its reverse complement primers. Subsequently, a hybrid assembly approach was applied, considering 97.4% of reads unmapped to the human genome as potential bacterial contaminants.