GSM8530915: 10-230-H3K27Ac; Homo sapiens; OTHER
100K cells were counted, resuspended in  wash buffer at room temperature, and added to activated ConA beads. Next, , anti-H3K27Ac (Cell Signaling Technology, 8173)  antibody was added at a final 1:50 dilution. Antibodies and permeabilized cells were incubated overnight at 4°C. Next, pAG-MNase was added, and the mixture was incubated for 10 minutes at room temperature, followed by 2 hours on a nutator at 4°C. Stop master mix was added, and samples were incubated at 37 ̊C for 10 minutes before CUT&RUN-enriched DNA fragments were purified with the Qiagen MinElute PCR purification kit (28006). DNA extractions were quantified using Qubit (Invitrogen). DNA-Seq library preps were constructed using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina kit (E7103) and amplified for 15 cycles.