GSM8128620: HUVEC, iMPs, 3; Homo sapiens; RNA-Seq
Total RNA from HUVEC treated with PBS, iMPs, PRP or FGF2 was isolated using an RNeasy Micro Kit (QIAGEN) ~10 ng total RNA was used as input for cDNA conversion using a SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (cat. #634890, Takara Bio). cDNA was fragmented using an S220 Focused-ultrasonicator (Covaris). The cDNA library was then amplified using a NEBNext® Ultra™ DNA Library Prep Kit for Illumina (cat. #E7370L, New England Biolabs).