GSM8264914: CD8 T cells, Day 8 Armstrong; Mus musculus; RNA-Seq
C57BL/6 mice (3 biological replicates) were infected with LCMV-Arm. At 8 dpi, mice were sacrificed, and single cell suspensions generated from spleens followed by filtration through a 70 mM filter. Spleen samples were split into two halves: the first half was subjected to CD45+ cell isolation (Miltenyi Biotec #130-052-301) and the second half used for CD8+ T cell isolation (negative selection kit, STEMCELL Technologies #19853). Single cell libraries were prepared using the 10X Genomics 5' v2 single-cell RNA-seq kit following manufacturer instructions (10X Genomics). Sequencing was performed on an Illumina NovaSeq 6000 S2 flow cell (Illumina Inc., San Diego CA, USA) using 125 cycles - 26 bp for read 1 containing the cell barcode (16bp) and UMI (10bp) and 91 bp for read 2, containing the biological read.