GSM7890222: hCO-D10-V190G-2; Homo sapiens; RNA-Seq
organoid pellet was lysed in lysis buffer(20mM Tris-Cl pH7.5, 5mM MgCl2, 150mM NaCl, 1mM DTT, 0.1% NP-40). Lysate was collected into 1.5mL tube and stay on ice for 15min. Lysate was then centrifuged at 21130g at 4°C for 10min to remove nuclei, mitochondria and debris. The supernatant was collected and the cytoplasmic RNA was extracted by Trizol-LS(ThermoFisher, 10296028) following the manufacturer's protocol. Ribo-minus TruSeq Stranded mRNA