GSM8589579: Patient_43B_Visium; Homo sapiens; OTHER
FFPE We used 10X Visium spatial transcriptomics platform (Visium CytAssist for FFPE Spatial Gene Expression 6.5mm, Human, 4 rxns[1000520], Dual Index Kit TS Set A, 96 rxn [1000251] and Visium CytAssist Spatial Gene Expression Reagent Kits, CG000495 RevE) to characterize the spatial gene expression patterns in four myeloid sarcomas in the skin. We followed all steps outlined in the 10X Genomics CytAssist Visium Protocol with overnight hybridization of ~18h. Tissue preparation, staining, and High-Resolution Scanning were performed by OSUCCC CPDISR. Library generation, FFPE tissue total RNA quality, and sequencing of the resultant CytAssist Visium Spatial Transcriptomic libraries were performed by OSUCCC GSR.