GSM8080347: nascent_DMSO_1; Mus musculus; RNA-Seq
Nascent RNA-seq: RNA was isolated using the RNeasy Plus Mini Kit (Qiagen). Total RNA was alkylated using 10 mM iodoacetamide and RNA was purified by ethanol precipitation. Nascent RNA-seq: Sequencing libraries were generated using the QuantSeq 3' mRNA-Seq Library Prep Kit (FWD) for Illumina.