RNA-Seq of Porites lutea: mucus
The DNA was extracted using the Qiagen DNeasy PowerSoil Pro Kit using the manufacturers protocol. ITSS rRNA genes from cDNA were amplified using the primers (core PCR primer in bold) CS1-5.8S-Fun-2-for (5-CAAGCAGAAGACGG CATACGAGAT-NNNNNNNNNNNN-AGTCAGTCAG-GG-AACTT TYRRCAAYGGATCWCT-3) and CS2-ITS4-FUN-2-rev (5=-AATGATACGGCG ACCACCGAGATCTACAC-TATGGTAATT-AA-AGCCTCCGCTTA TTGATATGCTTAART-3). The extracted DNA was then sent to the sequencing facility (Barts and The London Genome Centre, Queen Mary University of London) for quality control, PCR, library preparation, and pair-end sequencing with Illumina MiSeq platform v3 (2  300 bp).