GSM8343204: WM793, siRNA Caspase 3, replicate 2; Homo sapiens; RNA-Seq
Total RNA extraction was carried out using the Nucleospin RNA extraction kit (Macherey Nagel, 740955) according to the manufacturer's protocol.For RNA sequencing (RNAseq) analysis, libraries were constructed using 600 ng of total RNA per sample with the TruSeq Stranded mRNA kit (Illumina), following the manufacturer's guidelines. The process involved capturing PolyA mRNA using oligo-dT beads, synthesizing double-stranded cDNA, ligating adapters, amplifying libraries, and sequencing. Sequencing was performed using the NextSeq500 Illumina sequencer with 75 bp paired-end reads. Standard Illumina protocol, paired-end sequencing Sequencing was performed using a NovaSeq Illumina device (Illumina).