RNA-Seq of Human Dermal Lymphatic Endothelial Cells
RNA-Seq of Human Dermal Lymphatic Endothelial Cells
HDLEC were harvested from culture at 70-80% confluency by trysinization no particular treatment has been applied to the HDLEC cells RNA extraction was performed with a Biobasic EZ-10 Total RNA extraction kit (BS82322) according to manufacturer protocol RNA-seq paired-end libraries have been prepared according to Illuminaâ€™s protocol using the TruSeq Stranded Total RNA Gold library prep Kit (Illumina, San Diego, USA). Libraries quality was assessed using the HS NGS kit on the Fragment Analyzer (Agilent Technologies, Santa Clara, USA). Libraries were quantified by qPCR using the KAPA Library Quantification Kit (Roche, Basel, Switzerland) to obtain an accurate quantification.