GSM8129036: Mouse Peak EAE C3 WT Optic Nerve GEX, Rep 1; Mus musculus; RNA-Seq
Mice were euthanized with isoflurane overdose then eyes were enucleated and optic nerves detached and flash frozen in liquid nitrogen then stored in liquid nitrogen. Later, optic nerves were retrieved and pooled across mice (n = 5 mice, 10 optic nerves per biological replicate) then lysed and nuclei isolated with 10X Chromium Nuclei Isolation Kit. Following debris removal, nuclei underwent transposition for 1 hour at 37c then were loaded onto Chromium Next Gem Chip J and partitioned into GEMs on Chromium Controller. Both ATAC and Gene Expression libraries were generated according to 10X Multi-Ome recommendations snMulti-ome-seq (paired snRNA-seq and snATAC-seq)