RNASeq of Pinctada maxima mantle: Exposed to seismic energy
mRNA from the pooled oyster mantle samples were enriched using oligo(dT) beads, and rRNA was removed using the Ribo-Zero kit. The mRNA was fragmented randomly by adding fragmentation buffer, and cDNA was synthesized by using an mRNA template and random hexamers primer, after which a custom second-strand synthesis buffer (Illumina), dNTPs, RNase H and DNA polymerase I were added to initiate the second-strand synthesis. After a series of terminal repairs, A-ligation and sequencing adaptor ligation, the double-stranded cDNA library was completed through size selection and PCR enrichment.