GSM8477217: LNT3_3; Spinacia oleracea; RNA-Seq
Total RNA was extracted from each sample using using the Zymo Research Quick-RNA Plant Miniprep Kit. The mRNA was was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand of cDNA was synthesized using random hexamer primers, followed by the second strand of cDNA synthesis using dUTP for directional library. RNA libraries were prepared for sequencing using NovaSeq X Plus Series