GSM8264963: MOLM13, 5uM DB2115, 12hr PRO-seq rep2; Homo sapiens; OTHER
PRO-seq was performed in two biological replicates as previously described using approximately 20 million nuclei per run on with GTP, ATP, UTP, and biotin-11-CTP (PerkinElmer) using 0.5% Sarkosyl (Fisher Scientific) to prevent transcription initiation. RNA was reversed transcribed and amplified to make the cDNA library for sequencing by the Vanderbilt University Medical Center (VUMC) VANTAGE Genome Sciences Shared Resource on an Illumina NovaSeq 6000 (PE-150, 50 million reads).