GSM8591407: Normal, Input; Mus musculus; ChIP-Seq
Cells were crosslinked for 10 minutes in 1% formaldehyde at room temperature and quenched with 125mM Glycine. ChIP was performed as previously described in Carey (2009). Antibodies used: H3K4me1 (Abcam ab8895), H3K4me3 (Millipore, 04-745), H3K27me3 (Cell Signaling Technology, C36B11), H3K27ac (Abcam ab4729). Samples were further processed for high throughput sequencing. Sequencing was performed on Illumina HiSeq 2500 instrument resulting in approximately 30 million paired-end 50 bp reads per sample