GSM7835406: S. coagulans cells, Erythritol added, biol rep 3; Staphylococcus coagulans; RNA-Seq
Total RNA was extracted using the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. After removing rRNA in samples by riboPool (siTOOLs Biotech, Planegg, Germany), the NGS library was prepared by MGIEasy RNA Directional Library Prep Set (MGI Tech, Shenzhen, China) according to the manufacturer's protocol.