Nitrogen Non-Responsive genotype, 270 mg of N per Kg of sand, Leaf Base 0 segment, Indiv 9
Leaf segments from three-month-old plants were collected, and total RNA was extracted. mRNA containing polyA tails was captured using oligo(dT) magnetic beads. First-strand cDNA was synthesized using random primers. To ensure strand specificity, dUTP was incorporated during second-strand cDNA synthesis. Finally, Illumina adapters were ligated. The mRNA strand-specific Illumina kit was employed.