GSM8404651: 786-O-LM ATAC Rep1; Homo sapiens; ATAC-seq
Sample preparation for the assay of transposase-accessible chromatin with sequencing (ATAC-seq) was performed according to the Manual of the ATAC-seq Kit (Active Motif, 53150). Briefly, cells were collected, pelleted, and resuspended in ATAC lysis buffer. Cells were pelleted again, followed by tagmentation reaction in the master mix and purification by applying DNA purification binding buffer and column. Tagmented DNA was amplified by PCR using the i5/i7 indexed primer, followed by sequencing at the Next Generation Sequencing core facility in UT Southwestern.