GSM7925565: pooled activated T cells [TCR_rerun]; Mus musculus; OTHER
Activated T cells (CD19-CD3+CD44+CD62L-) were sorted from pooled dLN or pooled ndLN of 3 mice per group. T cells from 8 samples were labeled with 8 distinct hashtag oligonucleotides (HTO)-conjugated antibodies to MHC-II/CD45, after which all samples were pooled for 10X sequencing. scRNAseq libraries were prepared using a 10X Chromium single cell 5' v2 chemistry kit (10X Genomics), according to the manufacturer's protocol. TCRseq libraries were prepared using a 10X Chromium single cell V(D)J enrichment kit for mouse T cells (10X Genomics), according to the manufacturer's protocol.