GSM8172917: SUDHL2, Aiolos, CUT&RUN, DMSO; Homo sapiens; OTHER
0.01% digitonin was used to premeabilize the cells and Mnase was used to cleave antibody-bound chromatin NEBNext® UltraTM II DNA Library Prep Kit for Illumina from New England BioLabs. A maxiumum of 10ng was used as starting material. Libraries were amplified using 14 cycles on thermocycler. Libraries were purified using AmpureXP beads from Beckman Coulter. Libraries were validated using Agilent high sensitivity DNA kit