LuCaP 170.3, H3K27ac, ChIP, rep 1
PDX tumors were passaged in NOD scid gamma (NSG) mice at the NCI and propagated in vitro as 3-dimensional organoid cultures. PDXs were enzymatically degraded using the MACS human Tumor Dissociation Kit. Samples underwent crosslink reversal with RNase and proteinase K, and DNA sequencing libraries were prepared using the Accel-NGS-2S DNA Library Kit. Chromatin sample preparation was obtained using the Active Motif ChIP-IT High Sensitivity kit per manufacturer instructions. Samples were immunoprecipitated overnight using ChIP-seq grade H3K27ac antibody or HNF1A, bound to protein G dynabeads.  A fraction of sample was not exposed to antibody and used as input control. Libraries were sequenced using 100-bp paired end reads using NovaSeq 6000 on the Illumina platform at the CCR-Sequencing Facility.