GSM6996094: cancer stage IB1 [CR_5]; Homo sapiens; RNA-Seq
Total RNA was isolated using TriZOL reagent (Invitrogen). The quality and quantity of isolated RNA were assessed using Fragment Analyzer automated system (Advanced Analytical). cDNA libraries were constructed using TruSeq Stranded Total RNA (with RiboZero, Illumina) kit, reverse transcriptase SuperScript III (Invitrogen), and AMPure XP Beads (Beckman) following standard manufacturers' protocols The adaptors-ligated fragments were loaded onto the flow cell using MiSeq v3 sequencing kit; 75 bp end-reads were generated on the MiSeq platform (Illumina).