ChIP-seq H3K4me3 of Xenopus tropicalis: Osteoblasts tadpoles
Crosslinking was achieved by formaldehyde fixation, followed by glycine quenching. Subsequent steps involved cell homogenization, nucleus purification, and sonication for chromatin fragmentation. Sample preparation was carried out using the Magna ChIP TM A kit, and for the immunoprecipitation, antibody H3K4me3 (Abcam, Ab8580) was employed.