GSM8561086: HeLa Input ChIP-seq Replicate 1; Homo sapiens; ChIP-Seq
DNA from the INPUT and IER3 pulldown ChIP samples were extarcted using phenol-choloform extraction followed by Ethanol precipitaion and the final pellet was dissolved in nuclease free water. The DNA fragments were repaired, A-tailed and further ligated with Illumina adapter. The final DNA library was obtained by size selection and PCR amplification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina platforms, according to effective library concentration and data amount required.