GSM7173799: follicular granulosa cell layers-F4 RNA-seq replicate_1; Gallus gallus; RNA-Seq
total RNA was extracted from each sample using RNAiso Plus reagent (TaKaRa, Otsu, Shiga, Japan) following the manufacturer's instructions. Strand-specific libraries (total of 10 stages with three biological replicates in each stage) were generated after depleting rRNA by the Ribo-Zero™ Gold Kit (Illumina, San Diego, CA, USA). cDNA libraries were  sequenced on HiSeq X Ten (Illumina) platform to produce  150-bp paired-end raw reads.