Whole genome sequencing of Vibrio from biofilm in holding tank
A total amount of 0.2 g DNA per sample was used as input material for the DNA library preparations. Briefly, genomic DNA sample was fragmented by sonication to a size of 350 bp. Then DNA fragments were endpolished, A-tailed, and ligated with the full-length adapter for Illumina sequencing, followed by further PCR amplification. After PCR products were purified by AMPure XP system (Beverly, USA). Subsequently, library quality was assessed on the Agilent 5400 systemAgilent, USA and quantified by QPCR(1.5 nM). The qualified libraries were pooled and sequenced on Illumina platforms with PE157 strategy in Novogene Bioinformatics Technology Co., Ltd (Beijing, China), according to effective library concentration and data amount required.