GSM8219093: B6T; Homo sapiens; RNA-Seq
Extract tissue total RNA using miRNeasy Tissue/Cells Advanced Micro Kit (Qiagen). The concentration and RNA integrity were then determined by using Equalbit RNA BR Assay Kit (vazyme) and Qubit Fluorometer (Invitrogen). The samples that meet quality control requirements can be used for library preparation. Sequencing libraries were generated using MGIEasy RNA Library preparation kit (MGI) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample. Eventually, circularized DNA were sequenced with MGISEQ-2000 system.