Cas13-CDAR-S_Empty_a
Cells expressing SNAP-CDAR-S or Cas RESCUE-S were transfected with or without (snap) 2 -gRNA (2.5pmol) or gRNA expression vector (300 ng), respectively, targeting PPIB R7C in technical duplicatesunder constant Dox (10 ng/ml) induction. Total RNA was isolated with RNeasy MinElute Cleanup Kit(Qiagen) following manufacturers instructions. After DNase I (NewEngland BioLabs) digest, sampleswere again purified with RNeasy MinElute Cleanup Kit. Editing yields were first determined by SangerSequencing, as described above. Next Generation Sequencing was performed by CeGaT with aNovaSeq6000 (Illumina) to generate 50 Mio. 2x100 bp paired-end reads per sample. Library wasprepared with TruSeq Stranded mRNA Kit (Illumina).