Eukaryotic 18S rDNA V9 amplification from Arabidopsis thaliana leaves
Amplicon were generated in a 2 step PCR, including blocking oligos to minimize amplification of the plant and Albugo. Primers used for plant  Gc006 TGATGTATTCAACGAGTTCACACCTTGGCCGACAG and Gc008 TCTAAATGATAAGGTTTAGTGGACTTCTCGCGACG . Primers used for Albugo Gc014 ATGCGTTCAACGAGTTTAAAACCTTGACCGATAGG  and Gc016 TGGCGAGGTTAAAAAAAGTTCCCACAAATCCATTCGCAAT