GSM7746931: L1921-GM19_rep2; Saccharomyces cerevisiae; Candida albicans; ATAC-seq
M.CviPI Reaction Buffer), by resuspending nuclei with 25 µL 2× TD buffer (20 mM Tris-HCl pH 7.6, 10 mM MgCl2, 20% Dimethyl Formamide), 2.5 µL transposase (custom produced) and 22.5 µL nuclease-free H2O, and incubating at 37◦C for 30 min in a Thermomixer at 1000 RPM. Transposed DNA was isolated using the MinElute PCR Purification Kit (Qiagen Cat# 28004/28006), and PCR amplified for 10 cycles using the usual ATAC-seq settings. Libraries were purified using the MinElute kit, then sequenced on a Illumina NextSeq 550 instrument as 2x36mers.