RT_YAP1_1
Cells were fixed in 1% formaldehyde and resuspended in lysis buffer. Chromatin was sheared to 100-500 bp using a Branson Sonifier cell disrupter. Antibody and magnetic beads were coupled, then combined with solubilized chromatin. Target-chromatin complexes were pulled-down using magnet, washed and then eluted. After reverse cross-linking, RNase treatment, proteinase K treatment, immunoprecipitated DNA was extracted with phenol-chloroform, ethanol precipitated. One to ten nanograms of DNA were end-repaired, adapter-ligated and sequenced by Illumina NovaSeq 6000.