GSM8339582: WAT, HF_3; Mus musculus; RNA-Seq
For RNA-seq: Total RNA was extracted from the white adipose tissue of both the control and experimental (20mg/kg) groups of mice. Pair-end sequencing of the RNA was conducted using the Illumina platform (Illumina, CA, USA). For IGF2BP2 RIP-seq: Magna RIP RNA-Binding Protein Immunoprecipitation Kit(Merck sigma, USA) was used for IGF2BP2 RNA Immunoprecipitation (RIP). 3T3-L1 cells were lysed by RIP Lysis Buffer. Magnetic beads were co-incubated with IGF2BP2 antibody (Thermo Fisher Scientific, USA). Nucleic acid-protein immune complexes were collected by immunoprecipitation of lysates with incubated magnetic beads overnight at 4 °C. RNA on the protein A/G magnetic beads was collected and purified using proteinase K Buffer, etc., and then subjected to RNA high-throughput sequencing and biosignature analysis studies. RNA is extracted using the Trizol method and is quality checked before sequencing and before the RIP assay.