GSM8131978: P13,CiPDO,replicate1,RNA-seq; Homo sapiens; RNA-Seq
To isolate single cells, patient-derived organoids or CRC tissues were incubated with TrypLE Express at 37°C for 20-30 min. The cells were then filtered through a 20 μm cell strainer, after which live cells were counted using a hemocytometer. According to the manufacture ' s introduction, Single-cell RNA-seq libraries wereconstructed using Single Cell 3 ' Library andGel Bead Kit V3.1