GSM8619784: primary mouse chondrocytes, siPKM2_3; Mus musculus; RNA-Seq
RNA was first extracted from the cells using Trizol(Sigma). After quantification and quality control with ND 2000 (NanoDrop Technologies, Waltham, USA), 1 μg of highquality total RNA sample was used for the transcriptome library construction by Shanghai Majorbio Biopharm Technology (Shanghai, China). RNA libraries were prepared for sequencing using standard Illumina protocols