GSM7466945: ad-egfp3; Mus musculus; RNA-Seq
Total RNA was isolated using RNeasy mini kit (Qiagen, Germany). Paired-end libraries were synthesized using the TruSeq™ RNA Sample Preparation Kit (Illumina, USA). In brief, the mRNA was fragmented into small pieces following purification with poly-T oligo-attached magnetic beads. The cleaved RNA fragments were copied into first strand cDNA and the second strand cDNA was synthesized using DNA Polymerase I and RNase H. These cDNA fragments went through the end repair process, addition of a 'A' base, and ligation of adapters. The products were purified and enriched to creat the final cDNA library.