WGS of enterovirus:VRM1D/UFS-NGS/ZAF/2022
Extracted both DNA and RNA from faecal samples enriched for virus particles. The key enrichment steps entailed i) preparation of 10% faecal suspension, ii) homogenization of the faecal suspension, iii) centrifugation of the homogenate to pellet cellular debris, iv) filtration (0.45 micron filter) of the supernatant to eliminate larger bacterial and eukaryotic cells and v) digestion of free-floating unprotected nucleic acids. The virus particle-enriched samples were then used to extract DNA and RNA using a commercial kit. The extracted nucleic material was randomly amplified, followed by library preparation and high throughput sequencing on the Illumina Miseq platform.