GSM8129016: WT4; Mus musculus; RNA-Seq
ransfer to flow cytometry, and sort 2 * 106 GFP+B220+(182WT/182NC) BM cells to trizol. RNA was harvested using Rneasy mini plus kit (Qiagen). 1.3 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.