GSM7835505: Adult_rat, T cells,_HLA B27, H3K27ac, Replicate 1; Rattus norvegicus; ChIP-Seq
mLN Tn (2-7 x 105 cells) were sorted from 4 wk- or 3 mo-old rats, fixed using 1% paraformaldehyde (Electron Microscopy Sciences) for 8 min. After cell nuclei lysis, chromatin was fragmented (Bioruptor PICO/Diagenode), immunoprecipitated overnight at 4°C using rabbit polyclonal Abs: IgG (Diagenode, 1µg), m/r anti-H3K4Me3 (Diagenode, 1µg), m/r anti-H3K27Ac Abs (Active Motif, 5µg), then tagmented using the ChIP-mentation Kit for Histones (Diagenode). Libraries were prepared according to the ChIPmentation Kit guidelines (Diagenode) ChIPmentation