GSM8128605: mCherry - 1 d post-ONC replicate 2; Mus musculus; RNA-Seq
RGCs were isolated by a modified two-step immunopanning protocol based upon negative and positive selection with an anti-mouse macrophage antibody (Accurate Chemical, AIA31240) and an anti-mouse Thy1.2/CD90 antibody (Bio-Rad, MCA02R), respectively. Cells were loaded in parallel for capture onto the Chromium System using Chromium Next GEM Single Cell 3' Kits v3.1 (Dual Index, 10X Genomics, PN-1000269). Following capture and lysis, cDNA was synthesized and amplified for 12-14 cycles, as per manufacturer's protocol (10X Genomics). Amplified cDNA was used to construct Illumina sequencing libraries and sequenced by Hi-Seq 4000 100 bp paired-end sequencing.