GSM7835446: HFT CAT 3; Homo sapiens; RNA-Seq
Samples were stored at -80°C and then processed using the RNeasy Mini Kit (Qiagen). The connective tissues were cut in small pieces and digested by vortex in 2ml eppendorf with lysis buffer and a stainless steel bead. All the samples were homogenized into QIAshredder (Qiagen) by centrifugation. Total RNA was extracted according to the manufacturer protocol. mRNA 3'DGE research grade sequencing service (Next Generation Diagnostic srl)