16S sequencing of homo sapiens gut microbiome
Stool bacterial DNA was extracted using QIAamp Fast DNA Stool Kit (Qiagen, CA, USA). The V3-V4 region of the 16S rRNA gene was amplified using the primers S-D-Bact-0341-b-S- 17(5- TCG TCG GCA GCG TCA GAT GTG TAT AAG AGA CAG CCT ACG GGN GGC WGC AG- 3'), and S-D-Bact-0785-a-A-21 (5-GTC TCG TGG GCT CGG AGA TGT GTA TAA GAG ACA GGA CTA CHV GGG TAT CTA ATC C- 3') (IDT, IA, USA) MiSeq platform (Illumina, CA, USA) was used to perform high-throughput sequencing (2- by 300- bp paired-end). Sequences were processed with the Dada2 package (Version 1.10.1)