PacBio Sequel II sequencing of amplicons containing CRISPR/Cas9-induced 3 kb inversions
We isolated DNA from tomato protoplasts and subsequently amplified the samples using Phusion PCR. The PCR products were purified using the Zymoclean Gel DNA Recovery Kit. Following cleanup, the samples were barcoded and pooled equimolarly. These prepared samples were then submitted for high-throughput sequencing using the PacBio Sequel II systemm.