GSM8490020: hTERT, 16kPa, timepoint 1, RNA-Seq 1, replicate 3; Homo sapiens; RNA-Seq
Cells were lysed directly on cell culture plates in Buffer RLT Plus (Qiagen). The lysate was homogenized using Qiashredder columns (Qiagen) and stored at -80°C. Once all samples were collected, RNA was isolated using a RNeasy mini kit (Qiagen). Quality and concentration of RNA were determined using a 2100 Bioanalyzer Instrument (Agilent). cDNA libraries were constructed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina rRNA depletion workflow (New England Biolabs). Samples were sequenced on a NovaSeq 6000 (Illumina).