GSM7835400: DS06_CL_1; Homo sapiens; RNA-Seq
Total RNA was isolated from cells derived from freshly harvested cells from DS-ALL cell lines and PDXs using the AllPrep DNA/RNA Mini Kit (Qiagen). RNA integrity, quality and quantity were measured by TapeStation RNA ScreenTape (Agilent), Nadodrop and Qubit RNA BR Assay Kit (ThermoFisher), respectively.  All samples had a RIN number > 9.5. 500 ng of total RNA (RIN >9.5) per sample were used as input for library preparation using the SureSelect Stranded PolyA RNASeq kit (Agilent), according to manufacturer's instructions by Genomics WA.