GSM7466697: SVG+SOX10_wildtype_2_input; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 9 minutes. After quenching and cell lysis, chromatin was sonicated using a Corvaris S2 focused-ultrasonicator.  Chromatin immunoprecipitation was performed with the mouse anti-FLAG2 clone M2 antibody (Sigma cat # F1804) using the SimpleChIP Plus Sonication Chromatin IP Kit (Cell Signaling cat # 56383) following the manufacturer's instructions. Sequencing libraries were prepared from both total input DNA and following anti-FLAG immunoprecipitation using the KAPA Hyper Prep Kit (KAPA cat # KK8502) complemented with DNA adapter barcodes (KAPA UDI Adapters KK8727). 20 ng of DNA was used as starting material for input and IP samples. Adapter-ligated libraries were amplified for 8 cycles with HiFi Polymerase. Post-amplification cleanup and double-sided size selection was performed with KAPA cleanup beads. Sequencing library size and quality were checked by Agilent Bioanalyzer profiling, and sequencing libraries were also validated by qPCR using the KAPA Library Quant kit KK4824) before pooling for sequencing.