GSM8530604: formative ESC, double KO, rep1; Mus musculus; OTHER
EdU pulse-labeled Okazaki fragments were extracted from frozen and lysed cell pellets, isolated by ultracentrifugation on a 5-30% sucrose gradient, and purified by biotinylation-azide Click chemistry Library was constructed with adapter ligation, PCR amplification, and purification with SPRI beads. Quality testing of DNA was performed using Qubit and TapeStation analyses.