GSM8591111: mouse, Tgfbr1 KO, gastrocnemius, rep 1; Mus musculus; RNA-Seq
Both left gastrocnemius medialis and soleus muscles were dissected and frozen in liquid nitrogen. PolyA tail RNA was collected from each whole muscle. Muscles were homogenized by using TRIzol (Thermo Fisher Scientific). Ribopure Link (Thermo Fisher Scientific) and DNase I (Invitrogen) were used to isolate RNA. RNA purity was checked by 2200 TapeStation (Agilent Technologies), RIN values were all above 7.7. RNA concentration was measured by fluorometric quantification QbuitFluorometer (Life Technologies). Libraries were established following the protocol of Kappa-mRNA hyperprep kit (Roche Sequencing Solutions, Indianapolis, IN, USA).