GSM5709987: DSF06 pre-treatment; Homo sapiens; RNA-Seq
Single cell suspensions were prepared form tumor tissue specimens by enzymatic digestion and subjected to single-cell RNA preparation using BD Rhapsody. 10000 cells were loaded onto BD Rhapsody cartrige and captured cell number was asessed by BD Rhapsody scanner. Cell lysis, mRNA trap, cDNA synthesis, and primer digestion were performed according to the manufacture's instruction. cDNA amplification was performed by TAS-Seq protocol (Shichino et al BioRxiv 2021). Sequencing libraries from amplified cDNAs were produced by using NEB UltraII FS Kit (New England Biolabs) with some modifications. Sequencing was performed by using a Novaseq S2 200 cycle kit and a Novaseq 6000 sequencer (Illumina) according to the manufacture's instructions. 20% PhiX spike-in was used for sequencing. BD Rhapsody WTA