GSM8265210: CpG_2_IgG; Mus musculus; OTHER
Take out the cryovial and shake it in 37°C water bath to quickly thaw it within 1-2min.Cells were centrifugedfor 5min at 600×g at room temperature. Cell activity was detected with LUNA-FL TM and counted. CUT&Tag assay was performed as described previously (Kaya-Okur, Wu et al. 2019). Briefly, the cells are bound to ConcanavalinA-coated magnetic beads, and the cell membrane is permeabilized by Digitonin. The enzyme pA-Tn5 Transposaseprecisely binds the DNA sequence near the target protein under the antibody guidance and results in factor-targetedtagmentation. DNA sequence is tagmented, with adapters added at the same time at both ends, which can be enrichedby PCR to form the sequencing-ready libraries. After the PCR reaction, libraries were purified with the AMPurebeads and library quality was assessed on the Agilent Bioanalyzer 2100 system.