AMPLICON of Homo sapiens: human gut metagenome
The V4 hypervariable region was targeted. 100 pg of DNA was amplified using F515/R806 primers, which included Illumina adapters and a unique 8-nt sample index key. Amplicon libraries were pooled in equimolar amounts and purified using the QIAquick Gel Extraction Kit. Amplicon quality and size were analyzed on a Fragment Analyzer.