GSM8747107: T4 Cas9-4xBS bio rep 3; Arabidopsis thaliana; Bisulfite-Seq
Genomic DNA was extracted using DNA Extraction Buffer followed by isopropanol precipitation. DNA  was treated with RNAse A, recovered with phenol chloroform isolation then treated with sodium bisulfite using the EZ DNA methylation kit (Zymo Research). PCR-amplified amplicons were gel-purified and pooled in equimolar ratios. The Illumina Nextera DNA Flex kit (https://doi.org/10.1101/779215) was used to prepare the library. Single-end Illumina sequencing of 300 bp with an Illumina MiSeq.