GSM7926108: CD133highCD71lowFSClow; Mus musculus; RNA-Seq
Pancreata from adult normal mice (n=5 mice per experiment) were dissociated by collagenase B, and FACS sorted to obtain the CD133(hi)CD71(low)FSC(mid-high) (abbreviated as FSC-MH) or the CD133(hi)CD71(low)FSC(low) (abbreviated as FSC-L) sub-populations. These two sub-populations were then captured on a 10xGenomics Chromium controller using a Chromium single cell 3' library & Gel Bead kit V3 kit. Reverse transcription was performed in emulsion droplet with 10xGEM beads, full length cDNA was amplified and 3' expression library was constructed with Tn5 transposase based method (with 10xGenomics chromium single cell 3' Library Constraction Kit V2).