Genome sequencing of Candidozyma auris CBS19063
Genomic DNA was extracted according to Navarro-Munoz et al. 2019 Mycopathologia, 1000ng was used in the multiplex library preparation using SQK-NBD114.24 kit, library was loaded onto a MinION flowcell and analysis was performed with the GridION platform. Basecalling was performed using Dorado with dna_r10.4.1_e8.2_400bps_sup@v4.3.0. Reads with a Q of 10 and higher and a fragment length of 1100bp or higher were collected in a single FASTQ file and 50bp from each filtered read was removed from both ends. Data showed that barcode-leaking has occurred during the library prep/run, thus it can be expected that other microbial DNA is present at low percentage in the FASTQ data for this strain