GSM8746987: murine PATS-1; Mus musculus; RNA-Seq
For total RNA extraction, cells were resuspended in TRIzol reagent (Thermo Fisher Scientific, 15596026) and total RNA was extracted using a Direct-zol RNA Microprep kit (Zymo, R2061) with DNase I treatment according to the manufacturer's protocol. Purified RNA (1 µg) from each sample was enriched for Poly-A RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England BioLabs, #E7490). Libraries were prepared using NEBNext Ultra II RNA Library Prep Kit for Illumina (New England BioLabs, #E7770).