GSM7466559: WISC_3101; Homo sapiens; RNA-Seq
Coordinators obtained nasal cells and secretions by inserting a swab (#516CS01, Copan Inc., Murrieta, CA) into the nasopharynx, rotating it three times, and then immediately immersing it in a cell lysis buffer (RLT buffer, Qiagen, Ann Arbor MI) to preserved the RNA. The tube containing the swab tip was vortexed, kept at 4C overnight, and cryopreserved at -80C pending RNA extraction (RNeasy Micro Kit, Qiagen). RNA (1 ng) was fragmented, and cDNA was synthesized using random hexamers (SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian reagents, Takara Inc). Next, we added Illumina-compatible indexed adapters and enzymatically depleted host rRNA. A second round of amplification was used to generate the final sequence library.