GSM8128598: 20170525; Homo sapiens; miRNA-Seq
The peripheral blood samples from pregnant women with written informed consent were stored in EDTA tubes, then were centrifuged in 1,600g for 10 minutes to remove blood cells. The supernatant was transferred to a new EP tube and was centrifuged at 16,000g for another 10 minutes to obtain the purified plasma, which was stored at -80 °C. Then， total RNA was isolated by TRIzol® Reagent (Invitrogen) then the construction of cDNA library was performed by Illumina TruSeq Small RNA sample prep kit. The target PCR products were purified through 6% Novex TBE PAGE gel and the quality was checked by TBS380 (Picogreen). Then PCR amplification was performed and the generated clusters were sequenced by Illumina HiSeq platform.