GSM8532557: totalRNASeq_K365T_ID_LIF_rep1; Mus musculus; RNA-Seq
mESCs were dissociated using TrypLE, washed in 1X PBS, pelletted and 2.5 million cells were used for extracting RNA with RNeasy plus kit (Qiagen #74134) in each case. RNA quality was checked in Tapestation (Agilent High Sensitivity RNA ScreenTape) and quantified by Nanodrop. 500ng were used for Total RNA libraries preparation using Illumina kit (Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus #20040529). Libraries were then sequenced with the Novaseq6000 Illumina technology according to the standard protocols and with around 100 million 150bp paired-end total per sample.