Iso-Seq of female Z. viviparus muscle tissue
RNA was extracted from approx. 25 mg muscle tissue using the RNeasy Plus Mini Kit (QIAGEN). Full-length cDNA sequences were produced using the TeloPrime Full-Length cDNA Amplification Kit V2 (Lexogen). SMRTbell adapters were added with the SMRTbell prep kit 3.0 (PacBio), followed by RNA sequencing on PacBio Sequel II systems.