WGS (no USER) of Mammuthus primigenius DNA Zoo Sample6310
Just as in the case of data published in (Díez-del-Molino et al. 2023), DNA was extracted from a piece of the ethanol preserved tissue sample at the ancient DNA lab at the Swedish Museum of Natural History, Stockholm, Sweden. The tissue was digested overnight with the lysis buffer described in (Sinding et al. 2015). Next, the lysis was concentrated and purified as described in the supplementary protocol of (Dehasque et al. 2022). Double-stranded sequencing library was prepared following the protocol of (Meyer and Kircher 2010) with one exception. Specifically, we omitted the USER treatment during the blunt-end repair step to excise uracil bases from the DNA fragments as described in (Pecnerová et al. 2017).