GSM7835385: WT 21; Mus musculus; RNA-Seq
Total RNA was extracted from tissue samples, the concentration and purity of the extracted RNA was measured by Nanodrop2000, RNA integrity was measured by agarose gel electrophoresis, and RIN value was measured by Agilent2100. A single library construction requires a total amount of RNA ≥ 1ug, a concentration of ≥ 35ng/μL, OD260/280≥1.8, OD260/230≥1.0. Eukaryotic mRNA sequencing is based on the Illumina Novaseq 6000 sequencing platform, which sequences all mRNAs transcribed by specific tissues or cells of eukaryotes at a certain time, and the sequencing experiment uses the Illumina TruseqTM RNA sample prep kit method for library construction