PacBio Sequel II sequencing of amplicons that served as control
We isolated DNA from tomato protoplasts and subsequently amplified the samples using Phusion PCR. The PCR products were purified using the Zymoclean Gel DNA Recovery Kit. Following cleanup, the samples were barcoded and pooled equimolarly. These prepared samples were then submitted for high-throughput sequencing using the PacBio Sequel II systemm.