GSM8561446: BAAT-1, HTO; Mus musculus; OTHER
Livers were enzymatically digested using 0.5% type IV collagenase and DNase, followed by filtration with 70 µm cell strainers. Immune cells were further purified using 40% isotonic percoll and later incubated with ACK lysis buffer to lyse red blood cells. Immune cells.T cells (CD45hiCD3+) and all remaining immune cells (CD45hi) were sorted from each group, stained with CITE-seq antibodies and then subjected to scRNA-seq. Sorted cells were partitioned into an emulsion of nanoliter-sized droplets using a 10x Genomics Chromium Single Cell Controller and RNA sequencing libraries were constructed using the Chromium Single Cell 3′ Library & Gel Bead Kit v2 (10X Genomics, Cat# PN-120237). gene expression libraries were generated using 10X Genomics Chromium Single Cell 3' Library Construction Kits according to manufacturer protocols. 10x Chromium Single cell 3'  Kit cDNA, Illumina Novaseq 6000