RNA-Seq of Bone Marrow Derived Macrophages KISTINGr3
RNA for sequencing was extracted from homogenized Spleen, BMDMs and Microglia using Opti-prep RNA isolation kit (Invitrogen, #AM1924). RNA was sequensed by Novogene UK. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesizedusing random hexamer primers,  cDNA for the second strand synthesis was done using either dUTP for directional library or dTTP for non-directional library. Afterwards Endpair & A-tailing, Adapter ligation, Size selection and PCR amplification was done.