GSM8128807: ATAC_nave-KO_rep1; Mus musculus; ATAC-seq
ATAC-seq DNA library was constructed using TruePrep DNA Library Prep Kit V2 for Illumina and TruePrep Index Kit V2 for Illumina. Briefly, 15,000~50,000 sorted cells were resuspended in 35μL lysis buffer (10mM Tris-HCl, 10mM NaCl, 3mM MgCl2, 0.1% IGEPAL CA-630) and incubated on ice for 10 min with 3 times vortex. DNA fragmentation was conducted by adding 10μL 5 X TTBL, 5μL TTE Mix and shaking at 37℃ for 30 min. Fragmented DNA was purified using 1.8 X AMPure beads, barcoded with dual indexes and PCR amplified. Size selection and purification of DNA fragments were done using AMPure beads. Size distribution and molarity of the sequencing library were determined by Qubit (Thermo Fisher Scientific). Sequencing was performed using Illumina X-ten by Novogene. Every sample has two replicates.