Illumina MiSeq paired end sequencing
V1 to V3 region of the 18S rRNA using primers EukA7F and Euk570R
DNA extracts were prepared for paired-end MiSeq sequencing (Illumina, CA, USA) using a two-step PCR method to amplify the V1 to V3 region of the eukaryotic 18S rRNA  genes, and to attach MiSeq adapter and barcode sequences.