GSM7466288: M2_1_input; Rattus norvegicus; OTHER
MeRIP-seq and RNA-seq were conducted by LC-Bio Technologies (Hangzhou, China). Briefly, TRIzol (Invitrogen, USA) was used to extract total RNA from NP tissues. The RNA amount and purity of each sample was quantified using NanoDrop ND-1000 (NanoDrop, USA). The RNA integrity was assessed by Bioanalyzer 2100 (Agilent, USA) and confirmed by electrophoresis with denaturing agarose gel. Dynabeads Oligo (dT) 25-61005 (Thermo Fisher, USA) and Magnesium RNA Fragmentation Module (NEB, USA) were used to purify and fragment Poly (A) RNA. Then the cleaved RNA fragments were incubated with m6A-specific antibody (No. 202003, Synaptic Systems, Germany) in IP buffer to perform m6A RNA immunoprecipitation. Both input samples without immunoprecipitation and the m6A IP samples were used to construct libraries for MeRIP-seq. MeRIP