GSM7174097: S25: HLF Axl KO; Homo sapiens; RNA-Seq
Total RNA was isolated using the Monarch total RNA miniprep kit (New England Biolabs, Ipswich, Massachusetts, USA). Library preparation and sequencing was performed by Lexogen (Greenland, New Hampshire, USA). In brief, quality controlled and quantified RNA was used to prepare a QuantSeq 3' mRNA-seq (fwd) library suitable for Illumina sequencing, containing Unique Molecular Identifiers (UMIs).