GSM7467285: Control EPC2-hTERT organoids; Homo sapiens; RNA-Seq
For RNA extraction, Day 11 organoids were isolated from the Matrigel matrix, washed with PBS, lysed using Tri Reagent (Sigma-Aldrich) and stored at -80°C until further use. RNA was extracted for traditional RNA sequencing using RNeasy Mini Kit (Qiagen) according to the manufacturer's protocol RNA sequencing libraries of esophageal epithelial organoids were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina.