16S-ITS-23S of human: stool sample 37
The resultant 16S/ITS/23S amplicon pool (~2500bp) was used as a template to construct a SMRTbell library using Express Template Prep 2.0 (Pacific Biosciences)  as per the manufacturers Template Preparation protocol. The quality of the amplicon library was assessed using the Qubit 3.0 Fluorometer (Thermo Fisher Scientific Inc) and the Agilent Femto Pulse System (Agilent Technologies). The amplicon library was run on a single Sequel IIe system 8M SMRT cell using sequencing chemistry 3.0 with 4-hour pre-extension and 30-hour movie time.