Lupinus soil metagenome cf c2 forward
Microbial DNA was extracted with the QIAsymphony PowerFecal Pro DNA Kit (Qiagen, United States). DNA was amplified following the Illumina 15044223 B protocol of the 16S metagenomic sequencing library (Illumina, United States). Amplification indices were included in the second and final trial. 16S-based libraries were quantified by fluorimetry using the QuantiT PicoGreen dsDNA assay kit (Thermo Fisher Scientific, United States). The libraries were grouped prior to sequencing on the MiSeq platform (Illumina, United States), setting paired reads of 250 cycles. Cluster size and quantity were assessed in the Bioanalyzer 2100 (Agilent Technologies, United States) and Library Quantification Kit for Illumina (KAPA Biosciences, United States), respectively. Data quality assessment was performed with the MiSeq Control Software (MCS) v2.6.2.1 instrument.