GSM6998251: Arabidopsis tRNA TRM1A/B-3; Arabidopsis thaliana; OTHER
tRNAs were enriched from the soluble fraction of a LiCl precipitation, and then deacylated and dephosphorylated. Libraries were constructed accordingto a protocol derived from Behrens et al. 2021. An RNA adaptor was ligated at the 3' end of tRNAs. After purification with Zymo oligo D4060 (Zymo research), the adaptor-ligated-tRNAs were retro-transcribed with TGIRT III (Clinisciences), and the cDNA were purified. With Zymo oligo. The cDNA was then circularized using CircLigase (Euromedex) and submitted to Illumina indexing by amplification, using Illumina adapters. The DNA was purified with Zymo columns D4013. After quality control and quantification on Bioanalyzer (Agilent), the libraries were pooled with 35 ng per sample. This pool of samples was finally size-purified on a 6% polyacrylamide gel, quality was verified with a Bioanlyzer (Agilent) and quantified with a Qubit device (Thermo).