GSM8497184: Patient 19 Pre-Tx Tumor; Homo sapiens; RNA-Seq
Scroll (3-5, 20mm sections) from each FFPE block were used for total RNA extraction using the Qiagen RNeasy FFPE kit (cat#73504, Qiagen, Germantown, MD) in accordance with manufacturer's instructions. RNA quality was assessed using Qubit Fluorometric Quantification (Invitrogen) and Tapestation 4200 (Agilent). The samples were processed with the Illumina TruSeq RNA exome kit and sequence on one NovaSeq-600 SP-200 sequencing run.