Siha RNA-seq:OE NAT10
RNA-seq of Siha ShNAT10 vector and its OE-NAT10 cell lines was conducted by Guangzhou Epibiotek Co., Ltd., China. Briefly, library preparation was conducted using a VAHTS Stranded mRNA-seq Library Prep Kit for Illumina V2 (Vazyme Biotech, Nanjing, China, NR612-02) according to the supplier's protocol. Reads were aligned to the human Ensemble genome GRCh38 using Hisat2 aligner (v2.1.0) under the parameters: --rna-strandness RF. The reads mapped the genome were calculated using feature Counts (v1.6.3). The DEGSeq R-package was used to analyze differential gene expression.