RNA-seq of Camellia sinensis: midpoint between first and second shaking
Oligo (dT) was used to isolate mRNA from total RNA, and the mRNA was fragmented, reverse-transcribed to synthesize cDNA. cDNA fragments were further purified, processed by an end repair, single nucleotide adenine addition, and ligation of adaptors. Approximate 200 bp fragments were selected for the PCR amplification as sequencing templates.