GSM8530873: BALF, young mice, D7 post bleomycin; Mus musculus; RNA-Seq
Cells were pooled and sorted into PBS with 0.02% BSA to exclude erythrocytes and dead cells. Cell quality was checked, and concentration was adjusted to 800 cells/µl and 10,000 cells were loaded onto the 10x Chromium controller. Library preparation was performed using the Chromium Next GEM Single Cell 3′ Reagent Kits v3.1 protocol