GSM7117038: iWAT, WT_Cold, rep2; Mus musculus; RNA-Seq
Total RNA from WAT and iWAT was isolated using a commercially available kit (TIANGEN Biotech, DP430). Total RNA from other tissues including skeletal muscle, liver, BAT, heart, brain and small intestine was isolated following the standard method using TRIzol. RNA libraries for RNA-seq were prepared using SMART-RNAseq Library Prep Kit following manufacturer's protocols.