GSM8591378: Aggressive, Plag1 overexpression, replicate 3; Mus musculus; RNA-Seq
RNA was extracted using TRIzol LS Reagent Sequencing libraries were prepared using polyA-selection followed by NEBNext Ultra Directional RNA library preparation protocol (New England Biolabs). Sequencing was performed on Illumina HiSeq 2500 instrument, resulting in approximately 30 million 50 bp reads per sample.