GSM8002050: RNAseq_mouse_gastruloids_72h_1; Mus musculus; RNA-Seq
RNA was extracted using Quick-RNA MicroPrep kit (Zymo Research) according to the manufacturer's instruction with DNAseI treatment included. For RNA-seq, libraries were generated from 40ng (human somitoids or 150ng (mouse gastruloids) RNA with the KAPA RNA HyperPrep Kit with RiboErase (Kapa Biosystems). Fragmentation and priming were performed at 94°C for 6 min. NEXTflex DNA barcodes were used for adaptor ligation. Libraries were amplified with 11-14 PCR cycles followed by a two steps library amplification cleanup using a 0.8x bead-based cleanup and then an 1x bead cleanup.