DArTseq:Pleurobema sintoxia:raw Illumina reads
Sequencing for SNP genotyping was performed using DArTseq. Only PstI-SphI fragments were effectively amplified in 30 rounds of PCR. Equimolar amounts of amplification products from each sample of the 96-well microtiter plate were bulked and applied to c-Bot (Illumina) bridge PCR followed by sequencing on Illumina Hiseq 2500. The single read sequencing was run for 77 cycles.