16S V4V5 amplicon sequencing of volcanic sediments, Hunga Tonga Hunga Haapai: HTHH_2019_15
The V4-V5 region of the 16S ribosomal RNA gene was amplified using 515f/806r primers. Each primer combination had a unique barcode corresponding to a unique sample. Barcoded amplicons were normalized and pooled into a single library which was sequenced on a 2x150 Illumina MiSeq run. Sequences were subsequently demultiplexed by barcode into individual files corresponding to each sample.