16S RNA-Seq of Bacterial Community from Wetland Leaf Litter Layer
30ng qualified DNA template and the 16S rRNA fusion primers are added for Polymerase chain reaction (PCR). All PCR products are purified by Agencourt AMPure XP beads, dissolved in Elution Buffer and eventually labeled to finish library construction. Library size and concentration are detected by Agilent 2100 Bioanalyzer.