GSM8079237: Human aortic endothelial cells, 48h flow aligned, GSK205, rep1; Homo sapiens; RNA-Seq
Prior to cell lysis, non-aligned cells from the center of each well were removed using 1.8 cm blade cell scraper (Falcon #353085) and washed with 1X DPBS (Gibco #14190-144). The remaining flow-aligned cell monolayer was lysed using RLT lysis buffer (QIAGEN #79216) plus 1% 2-Mercaptoethanol (Sigma Aldrich #M6250). For each condition, 2 wells were combined as one flow-aligned sample for a total of 6 wells for n = 3 samples. Libraries for RNA sequencing were prepared using KAPA Stranded mRNA-Seq Kit (KAPA Biosystems #KK8421).