GSM8529246: CP_14_3; Cervus nippon; miRNA-Seq
Collect total RNA from chondrocytes and RMCs induced chondrogenic differentiation for 0, 7, 14 days  using Rneasy mini plus kit (Qiagen). A total amount of 3 μg total RNA per sample was used as input material for the small RNA library. Sequencing libraries were generated using NEBNext®Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA.) following manufacturer's recommendations and index codes were added to attribute sequences to each sample. the small RNA libraries were prepared for sequencing using standard Illumina protocols