ONT-Seq of Pachycara angeloi:  muscle
Total RNA was extracted from muscle tissue using the Qiagen RNeasy Mini Kit. mRNA was enriched with oligo(dT) beads and reverse transcribed to cDNA. The cDNA library was prepared using the Oxford Nanopore Technologies cDNA-PCR Sequencing Kit (SQK-PCS109) and sequenced on the PromethION platform using a PromethION Flow Cell (R9.4.1). Basecalling was performed with Guppy basecaller.