GSM7117002: Muscle, Chow diet, female, rep6; Rattus norvegicus; RNA-Seq
gastrocnemius tissue (50-100 mg) was homogenized in 1 mL of Trizol using the Bead Mill 24 . Total RNA was isolated using a combination of TRI reagent  and RNeasy-mini columns, including on-column deoxyribonuclease digestion. cDNA libraries for mRNA-sequencing were prepared using polyA-mRNA from individual RNA samples using Illumina TruSeq reagents