GSM8620550: Gag-hnRNPA1_PM_rep1; Homo sapiens; OTHER
UV-crosslinked cells  were fractionated via membrane flotation. Gag-RNA complexes were immunoprecipitated from the plasma membrane and Gag-bound RNA was extracted for CLIP-seq library construction. Libraries were constructed using sequential ligations of 3' barcoded adapter followed by 5' adapter. CLIP-seq libraries were PCR amplified using primers containing Illumina adapter sequences.