GSM8264909: Mononuclear cells, relapse, TMR, S18; Homo sapiens; ATAC-seq
The Active Motif ATAC-seq kit was used to perform ATAC-seq on MNCs. 1 × 10^5 cells were first pelleted and washed with ice-cold PBS. Subsequently, the cells were re-suspended in an ice-cold ATAC-Lysis buffer. Next, for tagmentation the isolated nuclei were incubated at 37°C for precisely 30 minutes. In ATAC-seq, treated DNA was purified using the Zymo DNA Clean and Concentrator-5 Kit. Subsequently, tagmented libraries underwent PCR amplification with 10 cycles and were size-selected using 60µl SPRI beads.