GSM8561357: U2OS, siG6PC3, REP1; Homo sapiens; RNA-Seq
Cells were collected 48 hours after transfection following RNA extraction using AllPrep DNA/RNA Mini Kit (QIAGEN, 80204) on Qiacube MDX. Subsequently, RNA was subjected to DNase treatment with RNase-Free DNase Set (QIAGEN, 79254) and RNeasy Mini Kit (QIAGEN, 74106) on Qiacube MDX. cDNA was converted and the libraries were pooled by Illumina Stranded Total RNA Prep with Ribo-Zero Plus kit (Illumina, 20040529) according to the manufacturer's protocol. The sequencing was performed on Illumina NovaSeq 6000. The 150 bp paired-end RNA-seq data was base-called and adapter-trimmed with bcl2fastq by the sequencing facility to generate the raw fastq files.