GSM8131851: Spatial_Transciptomics_Mouse_AicdaWT; Mus musculus; RNA-Seq
Tissues were decrosslinked and enzymatically permeabilized RNA libraries were prepared to add illumina indexes (i5 and i7) requested for sequencing, as reported in Visium Spatial Gene expression for FFPE protocol (10x Genomics Visium, CG000407) RHS and LHS probes for mouse samples from the Mouse Transcriptome Probe Kit (10x PN 2000457 and 2000458) were added to the visium slide, following the manufacturer's instructions, and then incubated for approximately 18 hours at 50 °C in a Thermocycler.