GSM8264918: aged_icm, scRNAseq; Mus musculus; RNA-Seq
Aged reprogramming cells from Day 14 post virus infection were harvested by trypsin and sent to Whan Biobank for library preparation The libraries were prepared according to the standard outlined in the manual. Briefly, single cells in nanoliter-scale oil droplets by Chromium Controller and to generate Gel Bead-In-EMulsions (GEMs). Full-length cDNA libraries were prepared by incubation of GEMs in a thermocycler machine. GEMs containing cDNAs were crushed and all single-cell cDNA libraries were collected together, and cleaned using DynaBeads MyOne Silane beads (PN 37002D; Fisher). The final constructed single-cell libraries were sequenced by Illumina Novaseq6000 machine (Illumina, San Diego, CA) with total reads per cell targeted, for a minimum of 50,000.