NextSeq 500 paired end sequencing
DNA isolation from the fresh-frozen tumor samples and white blood cell samples was  performed using a standard salt and chloroform based procedure. Isolation of DNA from FFPE tissue sections was performed with the Cobas DNA Sample Preparation kit art no. 05985536190 manual v 1.0 : Roche Molecular systems. Library prep and enrichment was done according the Twist Human Core Exome Panel exome enrichment protocol.