GSM6996045: Rep2_etoh_RNaseH_INPUT; Homo sapiens; OTHER
Chromatin was extracted from cells, sonicated, pre-cleared and this lysate was used for ChIP libarry prep.  For DRIP-seq, chromatin was extracted from cells and treated with RNaseI and proteinase K. Nucleic acids were isolated, sonicated, pre-cleared and RNA:DNA hybrids were IP'd with s9.6 antibody. Purified eluate was converted to dsDNA and this lysate was used for DRIP library prep ahead of sequencing. ChIP-seq libraries were prepared using NEBNext Ultra II DNA Library Prep Kit with 6-8 cycles of PCR. DRIP-seq libraries were prepared using NEBNext Ultra II DNA Library Prep Kit.