GSM8405876: TE5.MyP_2; Mus musculus; OTHER
In bulk analysis, genomic DNA was prepared from sorted cells (30,000 cells) by proteinase K treatment and heat inactivation; Single cells were captured using the 10x Chromium system and further processed following the Chromium Single Cell 3' Reagent Kit protocol. cDNA library were fragmented and the sequencing library was generated according to the Single Cell 3' Reagent Kit protocol. The PolyloxExpress cassette was amplified by PCR with the Polylox-specific primers.