GSM8219036: Plant tissue, 0 μM DNAN, rep1; Arabidopsis thaliana; RNA-Seq
Plant root and shoot tissue from the shake flasks was ground up in liquid nitrogen and RNA extracted using the EasyPure plant RNA extraction kit (Transgen Biotech). RNA libraries for RNAseq were constructed by poly(A) mRNA enrichment. 1 μg RNA was applied to the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs).