swab pool 5
cDNA of the RNA in the extract was prepared by reverse transcription using an oligonucleotide, with a specific nucleotide sequence (5 residues 1 to 20) and a random sequence with 8Ns (residues 21 to 28) at the 3 end. Second-strand synthesis was performed using Klenow fragment DNA polymerase. The resulting double-stranded cDNA was PCR amplified.   Amplicons were purified and then submitted to the Australian Genome Research Facility  for library preparation and NGS. The nucleic acid was subjected to Nextera XT library preparation protocol and sequenced using Illuminas MiSeq platform to generate 300 nucleotide paired-end reads.