GSM7747065: Wildtype2, CMT167 cell, scRNA-seq; Mus musculus; RNA-Seq
Single cell suspensions of tumor-infiltrating leukocytes were obtained by digesting tumors in 1.25 mg/mL collagenase IV (STEMCELL Technologies, Cambridge, MA), 0.025 mg/mL hyaluronidase (Sigma-Aldrich), and 0.01 mg/mL DNAse I (Sigma- Aldrich) followed by processing with the Miltenyi GentleMACS system (Miltenyi, Bergisch Gladbach, Germany). For single cell RNA sequencing or intracellular cytokine staining digests were further cleaned by magnet bead isolation of live cells using Miltenyi's Dead Cell Removal Kit. Barcoding and scRNA-seq cDNA library preparation were done using the Chromium platform from 10X Genomics.