GSM8333913: MCF7,IAA,rep1; Homo sapiens; OTHER
Genomic DNA was extracted by EasyPure Genomic DNA Kit (TransGen Biotech). Total RNA was extracted by Invitrogen TRIzol (Thermofisher) . PolyA RNA was extracted by NEBNext® High Input Poly(A) mRNA Isolation Module. CUT&TAG libraries were constructed with CUT&TAG kit (Vazyme, #TD903). AMUC-seq libraries were constructed with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina.