MinION sequencing
ssDNA was synthesized through primer extension by AbiA protein (with N-terminal His-tag) in the presence of equimolar mix of all for deoxynucleotides. Reaction was stopped by the addition of 40 mM EDTA and Proteinase K. Second strand was synthesized, then libraries were prepared using the ONT SQK-LSK-109 ligation-based DNA sequencing kit (Oxford Nanopore Technologies, ONT).Each sample was diluted, based on average size of particles, to final concentration of 200 fmol dsDNA. Each step of libraries preparation was performed according to a standard SQK-LSK-109 protocol, with one difference - only half volumes of enzymes and reagents were used. The concentration of prepared libraries was determined by Qubit fluorometer (Thermo Fisher Scientific). Between 5-50 fmol of the final prepared libraries were loaded onto a washed and primed FLO-MIN106D flow cell and sequenced on MinION Mk1B device (ONT).