GSM8560655: NSC,Homo,3; Homo sapiens; RNA-Seq
Total RNA was harvested using Rneasy mini plus kit (Qiagen). mRNA-Seq libraries (poly A enrichment) for hESCs were prepared using TruSeq Stranded mRNA library kit and sequenced at Roy J. Carver Biotechnology Center (CBC) at the University of Illinois at Urbana-Champaign (UIUC) for 150bp paired-end (PE) sequencing on an Illumina NovaSeq platform. mRNA-Seq libraries (poly A enrichment) for NSCs were prepared by Novogene for 150bp paired-end (PE) sequencing on an Illumina NovaSeq platform.