GSM8342372: RNA_hESC_OE OTX2_Rep1; Homo sapiens; RNA-Seq
Nearly 100 EGFP-positive hESCs were collected as one biological replicate for RNA-seq library construction. Cells were picked and transferred to lysis buffer, and poly(A)+ RNA was captured by oligo(dT) primers. First-strand cDNA was synthesized by reverse transcription and then amplified, purified and fragmented. Fragmented cDNA was processed to construct an RNA-seq library by using the NEBNext UltraII DNA Library Prep Kit (New England Biolabs, Cat# E7645L) according to the manufacturer's protocol for sequencing on an Illumina NovaSeq 6000 sequencer with a 150 bp paired-end sequencing strategy.