RNAseq of Rattus norvegicus:  SHAM Thalamus
RNA-seq libraries were prepared using 250ng of homogenized tissue RNA using the TruSeq Stranded mRNA Library Prep Kit (Illumina) following the manufacturer's instructions.  Quality was determined using D1000 ScreenTape on the TapeStation 4200 (Agilent Technologies) and quantity was determined using the KAPA Library Quantification Kit (KAPA Biosystems/Roche).  Libraries were normalized and pooled 5 libraries per lane and sequenced paired-end using 2x101 cycles on HiScan SQ platform (Illumina).