GSM8128610: patient 1, tumor, post-chemotherapy, scRNA-seq; Homo sapiens; RNA-Seq
Fresh tumor samples were dissociated using a Human Tumor Dissociation Kit (Miltenyi Biotec) according to the manufacturer's guidelines. Peripheral blood mononuclear cells were isolated using Ficoll-Paque Plus (Cytiva, Sweden). According to the manufacturer's instructions, single-cell RNA-seq libraries were prepared by using the Chromium Single Cell 3' Library and Gel Bead Kit. RNA transcripts from single cells were uniquely barcoded and reverse-transcribed within droplets. cDNA molecules were pooled and then subjected to end repair and adapter ligation. Next, the products were purified and enriched using PCR to create a final cDNA library. Finally, the libraries were sequenced using the Illumina sequencing platform.