GSM8559533: PBS_scBCR; Mus musculus; OTHER
Single-cell RNA sequencing (scRNA-seq) was performed on female C57BL/6 mice treated with PBS, as well as on Ad5XBB.1.5+RBDXBB.1.5-HR groups, including Sting-/- mice that also received Ad5XBB.1.5+RBDXBB.1.5-HR immunization as previously described. Forty-eight hours after the final vaccination, the mice were euthanized, and lung tissues were collected. The lungs were minced with scissors and treated with a tissue dissociation solution to generate a single-cell suspension. After treatment with a red blood cell lysis reagent, the suspension was filtered through 40-µm strainers and resuspended in PBS. Single-cell suspensions from four mice per group were pooled and loaded onto a 10x Chromium Single Cell and VDJ library construction system (10x Genomics, 5'scRNA+BCR). The concentration of single-cell suspensions was adjusted to 500–1200 cells/µl. Between 10,000 and 18,000 cells were loaded, with subsequent steps performed following the standard protocols provided by the manufacturer. 10X 5' scRNA+TCR