GSM8078772: WT (ttchem) replicate 1; Homo sapiens; OTHER
Cells were lysed with TRIzol (Invitrogen 15596026) directly on the plate to quench 4sU incorporation. Total RNA was extracted following manufacturer's instructions. A total of66ng of 4sU-labeled RNA per sample was used for stranded RNA library preparation using the KAPA RNA HyperPrep kit (Roche KK8540) following the manufacturer's instructions. Libraries were sequenced in a NextSeq550 illumina sequencer in 1x75bp mode