GSM7890171: CD70+, within 1 week of aGvHD onset, 848-P_rna; Homo sapiens; RNA-Seq
RNA was isolated using miRNeasy mini prep kit (Qiagen) from sorted CD70+ and CD70- T cells using BD FACS Melody according to manufacturer's protocol. Lexogen QuantSeq 3' (Illumina) kit was used to perform preparation of mRNA library. Libraries were prepared according to manufacturer's protocol using 10ng total input RNA and quantified using Qubit fluorometer