TTF1 control of LncRNA synthesis and cell growth delineates a tumour suppressor pathway acting directly on the  ribosomal RNA Genes
TTF1 control of LncRNA synthesis and cell growth delineates a tumour suppressor pathway acting directly on the  ribosomal RNA Genes
TTF1 conditional Clones C8 and C14 were cultured for >7days with 100ng.ml-1 doxycycline (Dox100), cells were then either harvested or cultures continued for a further 3, 5 or 7 days without doxycycline (Dox0). Cultures were then either harvested or 100ng.ml-1 doxycycline readded and cultures maintained for a further 3, 5, and 7 days (Dox100-Readdition). Total DNA was isolated using 2% NaSDS and 2mg.ml-1 Proteinase-K, phenol:chloroform extraction, treated with RNaseA and recovered by ethanol precipitation. WGBS/EM-Seq was performed using the NEBNext MethylSeq library protocol (McGill University and Genome Quebec Innovation Centre).