scNanoATAC-seq2 of Mus musculus: Early embryos, oocytes, granulosa cells and mouse embryonic stem cells
Each single cell was picked into a 200-ul PCR tube containing 2 ul ice cold lysis buffer and then subjected to transposition. And then to termination and digestion, incubated at 25 centigrade, 5 min; 55 centigrade, 30 min; 4 centigrade pause. Library was constructed by genomic DNA purification and amplification with cell barcodes. Long-read amplicons with different cell barcodes were pooled together and subjected to PCR amplification and purification. After that, each long-read amplicon fragment library was loaded into one R9 chip and sequenced on the Oxford Nanopore PromethION 48.