epicPCR of effluent WWTP Varazdin_blaOXA-48-like_day3
Cells were extracted from wastewater with centrifugation and encapsulated into polyacrylamide beads. Fusion PCR was performed to amplify and merge the desired antibiotic resistance gene and a part of the 16S rRNA gene (V4 region). After purification of PCR fusion products, blocking PCR was performed to remove unfused products. The blocking PCR products were purified and a final nested PCR was performed using Illumina TruSeq adapters.