PacBio HiFi WGS of Anastrepha ludens: brown adult male from GUA10 GSS
HMW DNA was extracted using the MagAttract HMW DNA Kit (QIAGEN). Extractions were cleaned with 2x bead cleanup and sheared to a mean size of 20 kb using the Megaruptor 2 (Diagenode). SMRTBell libraries were prepared with the SMRTBell Prep Kit 3.0 (Pacific Biosciences) and sequenced on a 24M SMRT Cell on a Revio system (Pacific Biosciences).