GSM8497194: psoriatic DRG1; Mus musculus; RNA-Seq
The RNA of the tissue and cells were extracted using Trizol reagent according to the manufacturer's manual. Followed by total RNA extraction from each of the samples using an RNASimple Total RNA Kit (Tiangen, Beijing, China). The RNA from one representative symptomatic sample was used to construct an rRNA-depleted library using the Ribo-Zero rRNA Removal Kit (Plant Leaf) (Illumina, San Diego, CA). The library was subjected to RNA-Seq using a BGISEQ platform (Shenzhen Huada Gene Science and Technology Service Co., Shenzhen, China).