GSM8620276: Day 8 FLT3L; Mus musculus; OTHER
Samples were collected and stained with TotalSeq™-B Mouse Myeloid Cocktail, V1.0 (Biolegend, cat# 199904) separately after 8 days of culture. Stained samples were adjusted to a concentration of 1500 cells/μL and loaded onto a Chromium controller (10X Genomics) as per manufacturer protocol for GEM generation and barcoding. Samples were prepared for sequencing using the 10X Genomics Single Cell NGS library prep platform in accordance with manufacturer's instructions for capture of 20,000 cells per sample. Reverse transcription, cDNA amplification and sequencing libraries were generated using 10X Genomics Single Cell NGS NSQ2K kit - P4 100 cycle v3. Across samples, cells were sequenced to a target depth of 20,000 reads per cell.