GSM7174106: Claustrum_Nurr1_3; Mus musculus; RNA-Seq
RNA extraction was performed with Qiagens RNeasy micro RNA kit. 1ng of RNA was used for cDNA and amplified for 15 cycles. cDNA libraries were made using the protocol Smart-seq2 for sensitive full-length transcriptome profiling in single cells. cDNA libraries were tagmented using home-made Tn5 enzyme and amplified for 11 cycles. Tn5 transposase and tagmentation procedures for massively scaled sequencing projects and Nextera dual indexes (Illumina).