GSM8265750: CTRL2_IFN_REP1; Homo sapiens; RNA-Seq
Cells were washed in PBS and quickly lysed in the lysis buffer of the MirVanva MagMax RNA isolation kit (Thermofisher) and stored at -20 oC until isolation. RNA was isolated manually according to the manufacturer's instructions. RNA concentration and integrity were determined using the Bioanalyzer (Agilent D2000). All samples had a RNA integrity number (RIN) score  of at least 8. Of these samples 300 ng of RNA were sent to BGI Hongkong for library prep and sequencing on the DNBSEQ platform to achieve paired-end and 150 bp read length. Strand-specific mRNA libraries were prepared at BGI, following their inhouse protocol for DNBseq platform