GSM8477760: Smartseq2_Lipstic_TdLN_412; Mus musculus; RNA-Seq
Freshly collected tumor-draining lymph nodes (tdLNs) and tumors were processed for single-cell isolation. Tumors were digested with collagenase D and DNase I, and lymph nodes were digested with collagenase D. Single-cell suspensions were then prepared, stained, and sorted by FACS based on surface markers, as detailed in the Methods section. Single-cell RNA-seq libraries were constructed using the Chromium platform (10x Genomics) following the manufacturer's instructions. The RNA from sorted cells was processed for library construction, aiming for specific read lengths for each segment of sequencing. Libraries for bulk RNA-seq were prepared using the Smart-Seq2 method.