GSM8560964: Hfx. volcanii cells, rosR deletion strain, whole cell extract control, rep3; Haloferax volcanii DS2; ChIP-Seq
chromatin immunoprecipitation (ChIP) and DNA extraction was conducted as previously described (Martinez Pastor M. et al., 2017). For ChIP-seq, Libraries were constructed using the KAPA Hyper Prep kit using Illumina TruSeq adapters. DNA library quality was assessed by Bioanalyzer using a High Sensitivity DNA chip (Agilent). Samples for each species were multiplexed according to the adapter mixtures and pooled. Each pool was run on a single lane on an Illumina HiSeq 4000 instrument by the Duke Sequencing and Genomics Techonology core facility