GSM7888132: PDGFRα+ cells, Mutant, biol rep4; Mus musculus; RNA-Seq
For isolation of RNA from mesenchymal progenitors of LA/BC muscles, three hundred PDGFRα+ cells were sorted to wells of 96-well plate containing 10 µL of the lysis buffer at 4˚C. The lysis buffer was prepared by mixing 1 µL of RNase Inhibitor (TaKaRa, Cat# 2313A) and 19 µL of 10X lysis buffer (TaKaRa, Cat# 635013) and diluted to 1X with nuclease free water. The 3'UTR RNA-Seq was conducted using the CEL-Seq2 protocol, with the exception of employing the Second Strand Synthesis Module (NEB) for double-stranded cDNA synthesis. The library was then amplified through 10 cycles of PCR without any sample pooling.