GSM8746870: mouse_D32_1; Mus musculus; RNA-Seq
For RNA-seq sample collection, we scraped cells at each time point that exhibited a relatively consistent and successful induction phenotype, minimizing the inclusion of cells with failed induction. For each sample, total RNA was extracted using TRIzol reagent (Thermo) and subsequently purified using the RNeasy Mini Kit (Qiagen), following the manufacturer's instructions. RNA-seq libraries were constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB England BioLabs, E7770L). Paired-end sequencing was performed using Illumina NovaSeq 6000 with 150 base pairs (bp) read length. For each sample, total RNA was extracted using TRIzol reagent (Thermo) and subsequently purified using the RNeasy Mini Kit (Qiagen), following the manufacturer's instructions.