GSM7747182: Tregs, mir342KO, rep 1; Mus musculus; RNA-Seq
RNA was isolated using the RNeasy Micro kit (Qiagen cat#74004). All subsequent sample quality assessment was performed on a Fragment Analyzer electrophoresis system (Agilent). Total RNA was normalized prior to oligo-dT capture and cDNA synthesis with SMART-Seq v4 (Takara cat#634892). The resulting cDNA was quantified using a Qubit 3.0 fluorometer (Life Technologies). Libraries were generated using the Nextera XT DNA library prep kit (Illumina). 25 million reads per sample was performed using a HiSeq2500 (Illumina) on a Rapid run flow cell using a 100 base pairs, paired end run.