GSM7747060: AhR control3,CMT167 cell, bulkRNA-seq; Mus musculus; RNA-Seq
RNA was extracted from three sets of CMT167Cas9 control, CMT167AhR-KO, reversed transcribed, and cDNA sequenced using the Illumina NextSeq 2000. Triplicate wells of each subline were harvested separately, and total RNA was extracted using the RNeasy Plus Mini Kit (QIAGEN, Hilden, Germany), according to the manufacturer's instructions. cDNA was generated using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Waltham, MA) following the manufacturer's instructions. cDNA was sequenced using the Boston University Microarray and Sequencing Core Facility. Between 18 and 28 million total read pairs (CMT167 lines) were obtained per sample. Quality metrics were similar across all samples with no technical outliers. RNA libraries for RNA-seq were prepared folllowing manufacturer's protocols. cDNA was generated using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Waltham, MA) following the manufacturer's instructions.