GSM7890543: IAIE_IC_1; Mus musculus; RNA-Seq
RNA extraction was performed using RNeasy plus mico kit (Qiagen) according to the manufacturer's instructions. Genomic DNA contamination was removed using Optimal DNA depletion columns (Qiagen). Libraries were prepared using the SMARTer stranded total RNAseq mammalian pico input kit (Takara) according to the manufacturer's instructions. 5 ng of total RNA was used for production of libraries. Total RNA-seq, 2 x 150bp paired-end sequencing performed on Novaseq6000 (Illumina)