Dovetail OmniC Sequencing of Lygodium microphyllum
To generate chromatin conformation data, we first isolated nuclei following the protocol from Workman et al. (2019). Approximately 1g of sterile leaf tissue was ground in liquid nitrogen for nuclei isolation; we ensured that intact nuclei were obtained by staining with DAPI and examining under a fluorescent scope. The pelleted nuclei were washed with PBS, snap frozen in liquid nitrogen, and then used as the input into the Omni-C Mammalian Cell protocol following the manufacturers protocol (Dovetail Genomics, CA, USA) using a 1:10 dilution of the nuclease enzyme mix and 25-30 min digestion.