Cab Sauv. Before Stress Control 3
Grape berries (seeds removed) were pulverized under liquid nitrogen using a mortar and pestle. Total RNA was extracted from three biological replicates, each replicate comprised of homogenate of 6 berries each, using a modified DEPC-CTAB protocol (Gasic et al., 2004), with three technical replicates performed for each biological replicate at the Before Stress, During Stress, and After Stress time points. RNA was quality-checked on an agarose gel and was quantified using a Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). Following quality validation and quantification using a Qubit Fluorometer (Thermo Fisher Scientific) and Agilent Bioanalyzer (Santa Clara, CA, USA), cDNA libraries were prepared from the RNA and sequenced by BGI Genomics (Hong Kong, China) on an Illumina HiSeq 4000 platform as 2150 paired-end reads.