GSM7747241: 4Cseq_Gata3-TSS_WT_Naive; Mus musculus; OTHER
Cells were crosslinked with 1% formaldehyde at room temperature for 10 min. Cross-linked DNA was digested with NlaIII then ligated using T4 DNA ligase followed by de-crosslink. DNA fragments were subjected to a second digestion by DpnII and circular ligation with T4 DNA ligase. Circular DNA were subjected to PCR reaction using inverse primers containing adaptors and indexes. 4C-seq libraries were sequenced on a NextSeq.