GSM8532189: T98G cells,vector-3; Homo sapiens; RNA-Seq
Trizol method (1) Treatment of cells: For adherant cells, the original cell medium was sucked away with a vacuum pump, then 1 mL Trizol was added, thoroughly mixed, and then blown with a pipette repeatedly.1.3 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.