RNA-seq of Blue Gourami brains etc
RNA-seq libraries were prepared at the Crown Genomics Institute of the Nancy and Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science using the in-house polyA-based RNA seq protocol (INCPM mRNA-Seq). Briefly, the polyA fraction (mRNA) was purified from 500 ng of total input RNA followed by fragmentation and generation of double-stranded cDNA. After cleanup with Agencourt AMPure XP beads (Beckman Coulter), end repair, A base addition, adapter ligation and PCR amplification steps were performed. Libraries were quantified by Qubit (Thermo Fisher Scientific) and TapeStation (Agilent). Sequencing libraries were constructed with barcodes to allow multiplexing on an Illumina NovaSeq 6000 machine, using an SP (100 cycles) kit. 100bp single reads were sequenced on 2 lanes.