RNA-Seq of adult female oocyte
Oocytes were treated with acidic Tyrodes solution to remove the zona pellucida, washed in PBS with 0.5% BSA, and stored in RNA lysis buffer at -80C. Single-cell RNA-seq was performed using flow cytometry. cDNA was synthesized, amplified, fragmented, and adapter-ligated with Tn5 transposase. Libraries were sequenced on an Illumina HiSeq X Ten, generating >40 million 150bp paired-end reads per oocyte.