Marker Frequency Analysis of Vibrio cholerae E7946 mutants (YAV20, AB14 and AB23)
Marker Frequency Analysis of Vibrio cholerae E7946 mutants (YAV20, AB14 and AB23)
Cells were grown in the MM (minimal media supplemented with fructose, with  or without arabinose (ara). Vibrio cholerae population were maintained in steady state in minimal medium (M9 + 0.2% fructose) at 30°C for 12h to 20h without (top) or with (bottom) arabinose (0,02% final concentration Sigma GenElute® bacterial genomic DNA kit 'Nextera DNA library preparation kit' (Illumina) following the manufacturer's recommendations.