GSM8267109: D458 cells, DMSO, 72 hours, replicate 3, RNAseq; Homo sapiens; RNA-Seq
Cells were collected in Qiazol and RNA extraction was performed using Rneasy Plus Mini Kit (Qiagen) RNA purity, quantity and integrity was determined with NanoDrop (ThermoFisher Scientific) and TapeStation 4200 (Agilent) analysis prior to RNA-seq library preparation.  The Universal Plus mRNA-Seq library preparation kit with NuQuant was used (Tecan) with an input of 200ng of total RNA to generate RNA-Seq libraries.  Paired-end sequencing reads of 150bp was generated on NovaSeq 6000 (Illumina) sequencer at a target depth of 80 million paired-end reads per sample.  Raw sequencing reads were de-multiplexed using bcl2fastq.