GSM8478696: TM3, nomal, RNA-seq, Rep2; Mus musculus; RNA-Seq
The RNA was extracted using the Dynabeads® mRNA DIRECT kit (Thermo Fisher, 61011) to efficiently remove ribosomal RNA and prevent any potential DNA contamination. The purified product was divided into two equal portions, one designated for RNA-seq analysis and the other allocated for MeRIP-seq experimentation. The RNA was initially fragmented to approximately 100-nt using a fragmentation kit (NEB, E6150S). A portion of fragmented mRNA was subjected to m6A immunoprecipitation (IP) using the EpiMark® N6-Methyladenosine Enrichment Kit (NEB, E1610S) following the manufacturer's protocol. The RNA libraries were prepared for both input RNA and m6A-enriched mRNA following IP, utilizing the VAHTS Universal V8 RNA-seq Library Prep Kit for Illumina (Vazyme, NR605-01) according to the manufacturer's instructions and sequencing using the illumina NovaSeq PE150. RNA-seq, MeRIP-seq