Helix pomacella isolate 6598 WGS
Sequencing libraries were prepared from DNA extracted from ethanol-preserved samples with a isopropanol precipitation-based protocol and sequenced on the Illumina NovaSeq X platform (150 bp paired-end). The reads were trimmed, filtered and deduplicated with TrimGalore (--length 140 -q 20 --stringency 1 -e 0.1) and fastp(--detect_adapter_for_pe --qualified_quality_phred 15 --unqualified_percent_limit 40 --n_base_limit 5 --length_required 15 --dedup --trim_poly_g).