GSM7890208: RNA_rep4; Homo sapiens; OTHER
We used the Qiagen AllPrep RNA/DNA kit to isolate genomic DNA and total RNA from each sample. We performed two separate DNase treatments of the total RNA to remove DNA contamination. We constructed sequencing libraries for the RNA and gDNA samples separately. We used a custom primer to reverse transcribe GFP RNA from total RNA, then performed nested PCR to add sequencing adapters and Illumina indexes. For gDNA samples, we used a similar set of primers to target integrated GFP sites and used nested PCR to add sequencing adapters and indexes.