GSM8497371: S5P_WT_siRNA_INTS8_rep1; Homo sapiens; ChIP-Seq
Cells were fixed with 1.66 mM disuccinimidyl glutarate (DSG) for 15 minutes, followed by washing and fixing with 1% formaldehyde for 8 minutes at room temperature. After quenching and cell lysis, cells were sonicated using a Covaris E220 sonicator for 4 minutes. dxChIP-seq samples were prepared using NEBNext Ultra II DNA Library prep kit and validated using tapestation HS D5000 kit.