mRNA-Seq of RL95-2 cells treated with JAr cell derived extracellular vesicles
Oxford Nanopore platform was used for the RNA sequencing due to its utility and affordability. We have used the MinION device with R9.4.1 (FLO-MIN106D) flow cells and the cDNA PCR Barcoding kit (SQK-PCB-109) with 100 ng of input RNA. The initial pore count of the flow cell was 897. Library was prepared to produce 500 bases per read (487  56). Sequencing time was 72-hours (with wash/ refill cycles every 24 hrs). Twelve samples were run through the flow cell with indexing. Demultiplexing, quality control and adapter trimming was achieved through the Oxford Nanopore  proprietary software.