Amplicon of bioreactor assembly: sediment extracted from bioreactor
DNA was extracted from sediment extracted from bioreactors, using a DNeasy PowerMax Soil Kit (QIAGEN). The V3/V4 region of the 16S rRNA gene of extracted DNA was amplified using primers 338F (5-ACTCCTACGGGAGGCAGCAG-3) and 806R (5-GGACTACHVGGGTWTCTAAT-3) on the Illumina platform by a NovaSeq 6000.