RNA-Seq of C. jejuni T1-21: aerosensitive strain for 6 hr in aerobic
Samples were collected at Times 0 hours, 0.5 hours, 6 hours, 12 hours, and 24 hours after transfer to aerobic conditions. Samples were pelleted and stored frozen in 700 microliters TRI reagent at -80 degrees Celsius. RNA was isolated using the Direct-zol RNA miniprep kit. Samples were purified of DNA using Turbo DNA-free kit. cDNA libraries were prepared using approximately 100 ng of RNA and the Illumina TruSeq Stranded mRNA Library Prep kit. Sequencing was outsourced to a service provider and conducted using Illumina Hiseq 4000 platform.