Synapta sp. transcriptome
Tissue samples were finely chopped, placed in RNAlater (Invitrogen) buffer solution, and stored at -80 C. RNA extractions were performed from Trizol (Thermofisher), using Direct-zol RNA Miniprep Kit with in-column DNAse treatment (Zymo Research). mRNA was isolated with Dynabeads mRNA Direct Micro Kit (Invitrogen). mRNA concentration was estimated using Qubit RNA broad range assay kit (Thermofisher), and quality was assessed using RNA ScreenTape with an Agilent 4200 TapeStation on an Agilent Bioanalyzer 2100. Libraries were prepared using a KAPA-Stranded RNA-Seq kit targeting a 200-300 bp insert size, and results were assessed using DNA ScreenTape (Bioanalyzer 2100). Libraries were sequenced in multiplexed (8 libraries per lane) pair-end runs using 150 bp paired end Illumina HiSeq 4000 at the UC San Diego IGM Genomics Center. To minimize read crossover, 10 bp sequence tags designed to be robust to indel and substitution errors were employed.