GSM8746475: Rx-GFP d6-2; Mus musculus; OTHER
RNA extraction was performed by combination of Trizol reagent (ThermoFisher Scientific) and RNeasy mini kit (QIAGEN). One microgram of poly(A)-selected RNA of Rx:GFP mRO (day 6) with replicates were subjected to the Nanopore direct RNA sequencing using kit (SQK-RNA002). Sequencing was carried out on a GridION Nanopore FLO-MIN106D flow cell for each library for ∼48h.