GSM8591094: Slide_seq_CTL_2; Homo sapiens; OTHER
Six optimal cutting temperature compound-embedded (OCT) brain tissue (n = 3 DS, n = 3 euploid) were obtained for Slide-Seq. Cryosectioning was performed with consideration of anatomical landmarks, specifically targeting regions where the ventricular zone or cortical layers were discernible. OCT-embedded brain tissue was sectioned at a thickness of 10 μm at -21°C using the CM1950 Leica cryostat and immediately mounted onto a 10x10 mm Slide-seq chip. In cases where the tissue covered less than 50% of the tile, an additional serial section was placed adjacently on the same tile to increase coverage. Libraries were sequenced on a Novaseq X flowcell 10B (100 cycles). Genome alignment, filtration, and normalization were performed using the Curio Seeker bioinformatic pipeline as per the manufacturer's recommendations.