GSM8078747: tamoxifen_rep1; Mus musculus; RNA-Seq
Cells were dissociated with Trypsin followed by staining for magnetic-activated and fluorescence activated cell sorting. CD45+ cells were isolated directly into TRIzol LS Reagent. RNA was isolated and concentrated with the RNA Clean & Concentrator-5 kit (Zymo) according to the manufacturer's instructions including DNase treatment. NEBNext Ultra II RNA Library PrepKit (performed by Novogene)