GSM8498468: lon1-1 replicate B; Arabidopsis thaliana; RNA-Seq
Total RNA was extracted using the Direct-zol RNA Miniprep kit (Zymo Research, Irvine, CA, USA) with an on-column DNase treatment from snap frozen powder of Col-0 and lon1-1 6-day old seedlings. The concentration of RNA samples was tested by Agilent 2100 Bioanalyzer (Agilent RNA 6000 Nano Kit) and the purity was tested by NanoDropTM.Library Construction by BGI (Beijing Genomics Institute, Shenzhen, China). The stranded RNA-seq libraries were generated at BGI (Beijing Genomics Institute, Shenzhen, China) using the in-house rRNA Removal Library Construction Protocol.