pooled ice metagenome file
DNA extracts from August 1st were selected for metagenome sequencing based on their high rRNA and DNA concentrations. The five cryoconite and five ice samples were pooled respectively and libraries were prepared using the Oxford Nanopore Technologies Ligation sequencing DNA V14 (SQK-LSK114) kit following the manufacturer's instructions. The two sample pools were sequenced on separate PromethION R10.4 flowcells, controlled by MinKNOW v.23.11.4.