GSM8131999: RNA_H295R_DMSO_rep1; Homo sapiens; RNA-Seq
RNA was extracted from cells at 80% confluency using TRIzol reagent and mRNA enriched by poly(dT) capture. RNA was fragmented, converted to DNA, then size-selected for 200-400 bp DNA fragments before PCR amplification. All libraries were barcoded and prepared for sequencing using standard Illumina protocols.