GSM8078675: Pds5-PK wt WCE; Saccharomyces cerevisiae BY4741; ChIP-Seq
S.cer and C.gla cells mixture  was crosslinked in 1% formaldehyde for 30min, then incubated at 4oC overnight. Cells were broken in lysis buffer using multi-beads shocker (Yasui-kikai). Cell extract was then processed to chromatin shearing by sonication (Sonifier Branson, 2508). Sequencing library was prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645). Library was processed to double-sided size selection using AMPure XP Reagent (Beckman Coulter, A63881). Library was validated by Agilent High Sensitivity DNA kit and quantified by KAPA Library Quantification Kits.