GSM8488963: SG-CK2; Luffa aegyptiaca; RNA-Seq
Total RNA was extracted from selected tissues using a plant-specific RNA extraction kit, ensuring minimal degradation and contamination. We then performed quality assessment of the isolated RNA with a bioanalyzer to confirm integrity and purity. Following DNase treatment to eliminate any genomic DNA contamination, we synthesized cDNA using a high-fidelity reverse transcriptase and carried out second-strand synthesis to obtain double-stranded cDNA. The cDNA was processed through end repair, A-tailing, and adapter ligation to prepare it for size selection. We selected cDNA fragments of an optimal size range and amplified them using PCR to create a library suitable for next-generation sequencing. The library was quantified and validated for quality and size distribution before being sequenced on an Illumina platform.