Paired read Illumina sequencing of Escherichia Coli US157 genome
Genomic DNA was extracted from each strain using a AccuPrep DNA Extraction Kit (Bioneer) per manufacturers protocol and submitted to SeqCoast for sequencing . Samples were prepared for whole genome sequencing using the Illumina DNA Prep tagmentation kit and unique dual indexes. Sequencing was performed on the Illumina NextSeq2000 platform using a 300-cycle flow cell kit to produce 2x150bp paired reads. 1-2% PhiX control was spiked into the run to support optimal base calling. Read demultiplexing, read trimming, and run analytics were performed using DRAGEN (v3.10.12) with default settings.