GSM8129000: CD45_B16F10_GEX; Mus musculus; RNA-Seq
tumors were digested using Liberase TL (100ug/mL) and DNaseI prior to labelled with hashtag antibodies and anti-mouse CD45 Gene expression and feature barcoding sequencing libraries were prepared using the 10x Genomics Single Cell 5' Reagent Kits v2 (Dual Index) following manufacturer user guide (CG000330 Rev B). The final libraries were diluted to ~10nM for storage. The 10nM library was denatured and further diluted prior to loading on the NovaSeq6000 sequencing platform (Illumina, v1.5 chemistry, 28bp/98bp paired end for gene expression and feature barcoding, 150bp paired end for TCR libraries).