GSM7747185: Chow eWAT; Mus musculus; RNA-Seq
Nuclei were isolated from frozen epididymal (eWAT) and inguinal (iWAT) white adipose tissues by pulverizing the tissues in liquid nitrogen and homogenizing in chilled Douncers. Next, the nucleus suspension was subject to nucleus sorting to remove debris and multiplets. Then, 16,000 nuclei (targeting 10,000) were loaded onto the 10X Genomics Chromium controller., Libraries were constructed using the Chromium Single Cell 3' Reagent Kit v3.3 and indexed using sample barcodes.