GSM8075926: dCas9-p300 I1417N; Homo sapiens; OTHER
Cells were harvested and prepared as per the 10X Genomics Single Cell Protocols Cell Preparation Guide. ~10,000 cells were captured per lane using a 10X Chromium device. One lane was used per construct (dCas9-p300 WT and dCas9-p300 I1417N). Cells were captured using a 10X Chromium chip using the Chromium Next GEM Single Cell 3' Reagents Kit v.3 with Feature Barcoding Technology for CRISPR screening. Final libraries were sequenced using a NovaSeq 6000 for each p300 screen. Gene expression and CRISPR Guide Capture transcript libraries were pooled at a 4:1 ratio for sequencing.