GSM8172468: 156_Mc1r LKO; Mus musculus; RNA-Seq
Total RNA was extracted using Direct-zol RNA miniprep (Zymo Research, CA, USA). Individual sample concentration was 50 ng/ uL Individual libraries had a concentratrion of at least 1-5 nM and 10 uLvolume. RNA libraries for RNA-seq were prepared using Illumina Stranded mRNA Preparation, Ligation, Kit (Illumina) according to the manufacturere's protocol