GSM8560399: NSC,WT,ZM11,2; Homo sapiens; OTHER
Around half a million cells were harvested and bound to Concanavalin A beads. These cell-bound beads were incubated with antibodies overnight at 4°C and treated with pAG-MNase for 1 hour at 4°C. Calcium was added to start pAG-MNase digestion for 30 minutes and CUT&RUN fragments were released and purified by phenol-chloroform extraction. DNA concentration was measured by Quantus fluorometer. CUT&RUN seq libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina and size selection was performed after PCR enrichment of adaptor-ligated library using MagBind Magnetic Beads. Libraries were sent to Novogene for premade libraries partial-lane sequencing aiming for 10-20million reads.