WGS_HiC
Hi-C libraries were constructed using an established protocol with modifications. The sample was ground in liquid nitrogen, cross-linked with 4% formaldehyde under vacuum at room temperature for 30 minutes, and the reaction was quenched with 2.5 M glycine. After chilling on ice, the sample was centrifuged, the pellet was washed with PBS, and subsequently, resuspended in lysis buffer. The supernatant was removed post-centrifugation. The nuclei were washed with NEB buffer, resuspended, and solubilized with SDS, then incubated at 65C. Triton X-100 was used to quench the SDS. An overnight digestion with DpnII was performed at 37C. DNA ends were marked with biotin-14-dCTP, and blunt-end ligation was conducted. The chromatin was ligated and cross-links were reversed by proteinase K treatment at 65C. DNA purification followed using phenol-chloroform extraction. Biotin from unligated ends was removed with T4 DNA polymerase. Sonication sheared DNA ends were repaired, and biotin-labeled Hi-C samples were enriched with streptavidin beads. A-tails were added, Illumina PE adapters were ligated, and the libraries were PCR-amplified and sequenced on an Illumina HiSeq 2000 platform.