GSM8072074: nonSMA15; Homo sapiens; RNA-Seq
Total RNA was batch-isolated using E.Z.N.A® Total RNA Kit (Omega Bio-Tek Inc.), treated with RNase-free DNase I (New England Biolabs Inc.), and further processed using RNA Clean & Concentrator (ZYMO Research Corp.). Only samples with RNA integrity number (RIN) >8 were used for library preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturer's protocol.