Long-read WGS of Trichogramma kaykai
DNA was extracted from 25 mg of whole insect tissues using the MagAttract High Molecular Weight kit (Qiagen), following manufacturers instructions. The DNA was concentrated to 25 uL using Sergi Lab Supplies magnetic beads and went through the PacBio SRE kit to deplete fragments shorter than 10kb. The sample was barcoded and library prepped with the ONT SQK-NBD114.24 kit. The libraries were sequenced on a P2 Solo instrument using PromethION 10.4.1 flow cells. Every 24 hours the libraries were recovered and flowcells were flushed with nuclease (EXP-WSH004 kit) and reloaded.