GSM7890175: CD70+, within 1 week of aGvHD onset, 693-CD70-P_tcr; Homo sapiens; OTHER
RNA was isolated using miRNeasy mini prep kit (Qiagen) from sorted CD70+ and CD70- T cells using BD FACS Melody according to manufacturer's protocol. QIAseq Immune Repertoire RNA (Qiagen) kit was used to perform TCR library preparation. Libraries were prepared according to manufacturer's protocol using 10ng total input RNA and quantified using Qubit fluorometer