Leaf Transcriptome Analysis of Wild-Type Drimia
Young leaves were flash-frozen in liquid nitrogen, and total RNA was extracted using the RNeasy Plant Mini Kit (Qiagen), followed by DNase treatment (TaKaRa). The RNA quality was evaluated using TapeStation (Agilent), yielding a 28S/18S ratio of 2.8 and a RIN of 8.7. The mRNA library was prepared using the Qiagen mRNA Library Kit, involving RNA fragmentation, cDNA synthesis, and adapter ligation. Sequencing was conducted on the Illumina NovaSeq 6000 platform, ensuring high-depth coverage for leaf transcriptome analysis.