GSM8478328: Monocytes, mono-culture, Normoxia, run 1; Homo sapiens; RNA-Seq
Cells from monocultures and co-cultures at normoxic and hypoxic conditions are then collected, stained for CD45 (BioLegend, USA) and sorted through FACS. The CD45+ sorted cells are then lysed for RNA collection and purification using RNeasy Mini kit (Qiagen, USA) as per the manufacturer's protocol. RNA libraries were prepared using the SMARTer RNA-seq Library Prep Kit following manufacturer's protocol