GSM8530692: WT1F-NeuN-005; Mus musculus; OTHER
Mice were perfused with 0.1M phosphate buffered saline followed by 4% formaldehyde. Tissue was post-fixed for 24 hours, embedded in paraffim, and sectioned at 5μm. Tissue was stained using 1:100 Alexa Fluor 488 conjugated anti-NeuN (Sigma-Aldrich; ABN78A4), 1:100 Alexa Fluor 594 (Abcam; ab269822) conjugated in-house to an anti-PV antibody, and 1:100 Alexa Fluor 647 conjugated anti-amyloid (Biolegend; 803020). Transcripts were recovered from PV+ nuclei, NeuN+ nuclei, amyloid+ nuclei, and then a triple negative (TN). As per manufacturer's instructions (NanoString GeoMX)