GSM8705846: Fresh sorted DN T cells from Donor 938 PBMC; Homo sapiens; RNA-Seq
RNA was extracted from samples in TRIzol using chloroform separation and precipitation with isopropanol 100%. Following washes with ethanol 75%, the RNA was diluted in RNAse-free water. DNAse and EDTA were added. Libraries were prepared using the KAPA RNA HyperPrep PolyA Single-End Read Kit.