GSM8128575: Tal1/.Lmo2 T-ALL#1, scRNAseq; Mus musculus; RNA-Seq
Freshly harvested thymus was mechanically dissociated in 20% FBS/PBS, subjected to RBC lysis with ACK buffer, to remove RBC. Cells were subsequently resuspended in PBS and stained with 7-AAD. Viable (7-AAD negative) singlets were sorted by FACS and loaded onto the Chromium Controlled (10X Genomics, 3'v1 and v3.1) to achieve a desired yield of 10^4 cells per sample. Library preparation was done according to manufacturers instructions. cDNA and Illumina libraries were quality-checked by Advanced Analytic Fragment Analyzer (UMass Chan Molecular Biology Core Laboratory) and subsequently sequenced on a HiSeq platform with paired-end sequencing (2×150 bp) performed by Genewiz/Azenta Life Sciences (Burlington, MA).