GSM8478215: LU65, scRNAseq; Homo sapiens; RNA-Seq
Cells were treated with sotorasib (0.2 μM) for 0, 1, 3, and 7 days. Post-treatment, the cells were subjected to single-cell RNA sequencing using the Chromium Single Cell platform (10× Genomics). Each sample reaction contained 2×10^6 cells, with Cell Multiplexing Oligo added for cell multiplexing using Feature Barcode technology. Cells were pooled to create single-cell gel bead emulsions (GEMs). Each GEM comprised barcoded Single Cell 3ʹ v3.1 Gel Beads and a Master Mix with Cell Multiplexing Oligo-labeled cells, generated by partitioning oil. After GEMs were generated, cDNA was synthesized and amplified according to the manufacturer's protocol. All DNA molecules from each GEM shared a common 10x Barcode. Single-cell sequencing libraries were created and sequenced from the DNA molecules and 10x Barcodes according to the instructions in the Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (10× Genomics).