GSM8172878: MI_NOC_NO_rep5; Rattus norvegicus; RNA-Seq
After euthanasia, heart were rinced in PBS, and snap-frozen. Total mRNA from remote areas were extracted from crushed snap-frozen heart tissue using Tri-Reagent RNA isolation reagent (Sigma Aldrich). RNA quality was monitored for quality control using Agilent Bioanalyzer Nano RNA chip and Nanodrop absorbance ratios of 260/280 nm and 260/230 nm. Library construction was performed according to the Illumina kit protocol (TruSeq stranded mRNA sample preparation). mRNA was enriched using oligo dT magnetic beads and chemically fragmented. The libraries were validated by qPCR and sized by Agilent Bioanalyzer DNA high sensitivity chip. The concentrations for the libraries were normalized and then multiplexed together. The concentration used for clustering the flowcell SP was 300 pM. The multiplexed libraries were sequenced on one lane using single-end 100 cycle chemistry for the NovaSeq 6000 (Illumina).