GSM7925353: BI_H3K27ME3_Rep1; Homo sapiens; OTHER
Briefly, 1×105 cells were treated with ConA beads, then incubated with primary antibody overnight at 4℃. Cells were reacted with secondary antibody for 1 h after being washed for three times. CUT&Tag assay was performed using Hyperactive® Universal CUT&Tag Assay Kit for Illumina (Vazyme) as indicated. Samples were incubated with pAG-Tn5 for 1 h. DNA tagmentation was performed at 37 °C for 1h and followed by DNA extraction. PCR was applied to amplify the libraries followed by product purification. Sequencing and data analysis were performed by Novogene Co. Ltd using Novaseq-PE150 (Illumina).