RNA-seq of Pilocarpus microphyllus: tissues from a young greenhouse specimen
We collected fresh leaflet (4 replicates), rachis (3 replicates), root (2 replicates), and stem (2 replicates) of Pilocarpus microphyllus from a specimen maintained in our greenhouse. Samples (n=11) were collected in cryotubes containing RNAlater (Thermo Fisher Scientific), immediately frozen in liquid nitrogen, and stored at -80C. Total RNA was extracted using RNeasy Plant Mini Kit (Qiagen) and evaluated using Qubit (Thermo Fisher Scientific) and 4200 TapeStation System (Agilent Technologies). RNA libraries were made using TruSeq Stranded Total RNA with Ribo-Zero Plant (Illumina) and sequenced on NextSeq 2000 (Illumina) using a P3 Reagent 200 cycles (2 x 101 bp) (Illumina)