Characterization of aldosterone-producing cell cluster (APCC) at single-cell resolution
Characterization of aldosterone-producing cell cluster (APCC) at single-cell resolution
Adult human adrenal samples for scRNA-seq were collected from two Saitama Medical University International Medical Center cases. Samples 1 and 2 were obtained from a 62-year-old male at radical nephrectomy for renal cell carcinoma and a 29-year-old male at adrenalectomy for pheochromocytoma. CD24/CD31/CD45 positive cells were removed using FACS and adrenocortical cells were enriched. The libraries of scRNA-seq were prepared from the cell suspensions using Chromium controller (10X Genomics) and Chromium Single Cell 3' Reagent Kits v3.1 according to the manufacturer's instructions. The libraries of scRNA-seq were prepared from the cell suspensions using Chromium controller (10X Genomics) and Chromium Single Cell 3' Reagent Kits v3.1 according to the manufacturer's instructions.