GSM8267003: BMA41 mub1delta, CFW20, rep1; Saccharomyces cerevisiae; RNA-Seq
Total RNA was isolated by the hot phenol method, as in https://doi.org/10.1093/nar/18.10.3091, precipitated by ethanol, digested by the RNase-free DNase I, and purified by the phenol-chloroform treatment. RNA was used to prepare stranded, polyA-enriched True-seq libraries and sequenced them with NovaSeq 6000 (2x 150 bp) following the manufacturer's instructions.