Genome sequence of N2-fixer Stutzerimonas stutzeri strain MBI-RS3
Genomic DNA was prepared for sequencing using the Illumina DNA Prep kit (formerly Nextera DNA Flex, Catalog #20018704), following the manufacturer's protocol. Approximately 100 ng of high-quality genomic DNA was used as input. The DNA was tagmented using bead-linked transposomes, which simultaneously fragment and tag the DNA with adapter sequences in a single step. Following tagmentation, the fragments were amplified through a limited-cycle PCR to add unique dual index adapters, facilitating multiplexing. The resulting libraries were purified using Sample Purification Beads (SPB) and assessed for quality and quantity using a Qubit fluorometer. The final libraries were normalized to the recommended concentration and pooled for sequencing on an Illumina platform.