GSM7746857: SRK018-64; Mus musculus; RNA-Seq
The gastrocnemius muscle was dissected and placed in RNAlater (Qiagen Inc., Germantown, MD) overnight at 4 °C. RNAlater was removed the following day, and the muscle was frozen at−80°C until RNA isolation. The muscle was homogenized and RNA was extracted via miRNeasy extraction per manufacturer's instructions (Qiagen Inc., Germantown, MD). mRNA-seq libraries were structured according to the standard Illumina TruSeq-stranded mRNA protocol from 2 rounds of the same experiment run together