GSM8532538: TSC2null_DMSO_Total_rep1; Homo sapiens; RNA-Seq
Fractions corresponding to mRNA associated with more than two ribosomes were pooled and the RNA extracted using TRIzol (ThermoFisher) according to the manufacturer's protocol. 10% of the lysate was removed from the extract prior to loading on the sucrose gradient, extracted using TRIzol, and denoted as total RNA. Illumina v2.5 Kit