GSM6886457: N2-2; Caenorhabditis elegans; RNA-Seq
Total RNA was extracted from the worms using TRIzol ® Reagent according the manufacturer's instructions (Invitrogen Cat #15596018). RNA concentration and purity was measured using NanoDrop 2000(Thermo Fisher Scientific, Wilmington, DE). RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA). RNA libraries for RNA-seq were prepared using  VAHTS Universal V6 RNA-seg Library Prep Kit for Illumina® Kit following manufacturer's protocols.