GSM8075895: shNS; Homo sapiens; RIP-Seq
Immunoprecipitated samples were subjected to Proteinase K digestion at 55°C for 1 hour. Total RNAs were extracted from both the input and immunoprecipitated samples by adding 5 volumes of TRIzol reagent, followed by Direct-zol RNA Miniprep (Zymo). Libraries for high-throughput sequencing were constructed using the TruSeq Stranded v2 mRNA Sample Prep Kit (Illumina) and were quantified by a BioAnalyzer High Sensitivity DNA chip.