GSM7117394: WT_TSB_DIP_1; Staphylococcus aureus; RNA-Seq
Cell pellets were thawed and washed twice with 0.5mL of lysis buffer (20mM RNase-free Sodium acetate, 1mM EDTA, 0.5% SDS). The cells were lysed by the addition of 4μg lysostaphin and incubated for 40min at 37°C until confluent lysis was observed. RNA was isolated using TRIzol reagent according to the manufacturer's instructions Library construction was performed using the Illumina TruSeq Stranded mRNA Sample Prep Kit