GSM7747574: Control RV [CTRL_3]; Rattus norvegicus; RNA-Seq
RV tissues from PAB-treated rats were harvested after RHC measurements and snap-frozen. Total RNA was isolated from RV samples using miRNeasy Micro Kit (Qiagen) and snap-frozen. Purification of total RNA was performed as described in the RNeasy handbook, using DNase I digestion (RNase-Free DNase Set, Qiagen). RNA and library preparation integrity were verified with LabChip Gx Touch 24 (Perkin Elmer), and 2 µg of total RNA was used as input forVAHTS Stranded mRNA-seq Librarypreparation following manufacture's protocol (Vazyme).