Benthogone sp. transcriptome
Tissue samples were finely chopped, placed in RNAlater (Invitrogen) buffer solution, and stored at -80 C. RNA extractions were performed from Trizol (Thermofisher), using Direct-zol RNA Miniprep Kit with in-column DNAse treatment (Zymo Research). mRNA was isolated with Dynabeads mRNA Direct Micro Kit (Invitrogen). mRNA concentration was estimated using Qubit RNA broad range assay kit (Thermofisher), and quality was assessed using RNA ScreenTape with an Agilent 4200 TapeStation on an Agilent Bioanalyzer 2100. Library preparation and multiplexed pair-end sequencing (4 libraries per lane) on an Illumina NovaSeq 6000 PE150 were performed by Novogene.