GSM8267158: Donor 3 H413-CM moMO; Homo sapiens; RNA-Seq
Total RNA from monocytes was isolated using the PureLink™ RNA Mini Kit (Invitrogen) following manufacturer's instructions. In brief, monocytes were lysed and nucleic acids were loaded into RNA binding columns. Columns were washed, and total RNA was eluted. RNA samples were sent to BGI China. RNA libraries for RNAseq were constructed using DNBSEQ high-throughput platform. Sequencing libraries were constructed from ≥ 500 ng of total RNA.