polysome-IP profiling experiment : Polysomes of untreated or tunicamycin-treated AC16 cells were immunoprecipitated with anti-MRPS15 antibody, followed by RNA sequencing
polysome-IP profiling experiment : Polysomes of untreated or tunicamycin-treated AC16 cells were immunoprecipitated with anti-MRPS15 antibody, followed by RNA sequencing
AC16 cells were cultured in two 150 mm dishes per point. Ten minutes prior to harvesting, cells were treated with cycloheximide at 100 mg/mL. Cells were washed twice with PBS at room temperature containing 100 mg/mL cycloheximide and harvested at 80% confluence with Trypsin. tunicamycin has been applied to the cells at a concentration of 2 microgramms per milliliter for 4h at 37Â°C Standard extraction with Trizol has been applied according manufacturer recommandations. Library has been constructed according internal Genewiz/azenta pipeline. (genewiz.com)