GSM8621250: PapOVA d1 MHCIIhi CD11c+ B220- cells, GX + ADT; Mus musculus; OTHER
MHCIIhi CD11c+ B220- cells were sorted from popliteal lymph nodes of immunized and naïve mice and used to generate Gel Beads-in-Emulsion (GEMs) using the Chromium Single-Cell 3' Reagent v3 kit (1000092, 10X Genomics) according to the manufacturer's protocol. Cell suspensions with a >90% viability was mixed with RT reagents and loaded onto Chromium Chip B, PN-1000074 along with Gel beads and Partitioning oil in the recommended order and then the chip was processed through 10X Chromium Controller for the generation of GEMs, followed by RT, Cleanup, and cDNA amplification. After the cDNA amplification, size selection was used to separate the cDNA molecules for 3' Gexp (Gene Expression) library and ADT library construction