GSM8219394: RWPE1, enriched, scATAC, rep1; Homo sapiens; ATAC-seq
The multiome libraries were prepared according to the manufacturer's protocol. Briefly, nuclei were isolated from the dissociated cells and the open chromatin regions were tagged with Tn5 transposase. The tagmentated nuclei of each cell line was then applied to one lane of the Chromium Next GEM Chip J for GEM generation and molecule barcoding. After GEM incubation and library construction, each ATAC-seq read was labeled with a 10x cell identity barcodes, while each RNA-seq read was captured by a paired barcodes with an Unique Molecular Identifier (UMI). For raw multiome library capture, we aimed 8,000-10,000 nuclei for each sample and 25,000 scATAC-seq, and 20,000 scRNA-seq reads per cell to ensure the data coverage. We aimed for similar total read count for each targeted enriched library for better comparisons. After GEM incubation and library construction, each ATAC-seq read was labeled with a 10x cell identity barcodes, while each RNA-seq read was captured by a paired barcodes with an Unique Molecular Identifier (UMI). For raw multiome library capture, we aimed 8,000-10,000 nuclei for each sample and 25,000 scATAC-seq, and 20,000 scRNA-seq reads per cell to ensure the data coverage. We aimed for similar total read count for each targeted enriched library for better comparisons.