GSM8172745: PDO37_ZT4_S1; Homo sapiens; RNA-Seq
For RNA extraction, cells and organoids were cultured in a 6-well plate followed by dexamethasone treatment. 24 h after the synchronization, cells were collected and lysed with RLT buffer containing 1% 2-mercaptoethanol (Bio-Rad, CA). According to the instructions, RNA was isolated using RNeasy® extraction kit (Qiagen, Germany). RNA concentration was measured using a NanoDrop 2000c spectrophotometer (Thermo Scientific, MA). cDNA was prepared from the purified RNA using a cDNA Reverse Transcription Kit (Applied Biosystems, United States).