WGS_ONT
Genomic DNA was fragmented using a Covaris g-TUBE, followed by size verification. The DNA underwent repair and end-preparation with NEBNext End repair/dA-tailing Module and FFPE DNA Repair Mix, followed by purification with AMPure XP beads. Adapters from the NEBNext Quick Ligation Module were then ligated to the prepared DNA, with another round of AMPure XP bead cleaning, targeting specific fragment lengths using L or S Fragment Buffers. The DNA was finally eluted in Elution Buffer. This prepared library was loaded onto an Oxford Nanopore Technologies sequencer for sequencing. Each step included precise mixing and incubation according to the protocol.