GSM8532340: ChIPseq, Liver, H3K4me3, P6; Mus musculus; ChIP-Seq
Cells and liver tissue cell suspensions were fixed with 1% PFA for 15 minutes at RT , Nuclei extraction, quantification and ChIP was performed using the published RELACS protocol (Arrigoni et al, 2018) Antibodies: KANSL3 ( Sigma HPA035018), H3K27ac (Diagenode C15410196), H3K4me3 (Diagenode C15410003), H3K9ac (Cell Signaling 9649), H4K16ac (abcam ab109463, Hepa1-6), H4K16ac (Milipore 07-329, liver tissue). Libraries were prepared using a NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, E7645)