GSM8478430: 200553C_TM; Homo sapiens; RNA-Seq
4 mm punch biopsies were obtained from healthy whole skin specimens from the forearm and buttock region. Immediately after resection of subject, samples were kept in MACS Tissue Storage Solution (Miltenyi Biotec, cat. no. 130-100-008) for no longer than 1 h before their enzymatical and mechanical dissociation with the Whole Skin Dissociation kit for human material (Miltenyi Biotec, cat. no. 130-101-540) and the Gentle MACS dissociator (Miltenyi Biotec), following the manufacturer's instructions. Cell suspensions were then filtered through 70 µm cell strainers (Falcon) and depleted of cell debris and dead cells with the Dead Cell Removal Kit (Miltenyi Biotec, cat. no. 130-090-101). Raw sequencing data was processed with Cell Ranger, version 3.1.0, from 10X Genomics.