GSM8264915: Uchl1_KO; Mus musculus; RNA-Seq
Testicular tissue samples from Uchl1_KO mice were collected, and the frozen testicular tissues were handed over to Guangzhou Gene Denovo Biotechnology Co. for nuclei isolation, library preparation and sequencing according to the guidelines of 10 × Genomics (10 × Genomics, USA). About 300 mg of tissues from three mice were combined and dissociated into a mononuclear suspension. Cell nuclei were passed through a 40 × 10-6 M cell filter for removal of cellular debris and larger clumps. Nuclei concentration was manually assessed using 0.4% Taipan blue restaining and a blood cell counter. The nuclei concentration was adjusted to 700-1200 nuclei/μL, and the cell suspension was loaded onto a 10 × Genomics GemCode Single Cell Instrument and subjected to cell capture and library preparation according to the Chromium Next GEM Single Cell 3′ kit v3.1 instructions. Because a large amount of snRNA-seq data on WT mouse testes have been published, we searched the database to find the testicular snRNA-seq data of WT mice with the same age, strain, sequencing platform, and sequencing method as the Uchl1_KO mice in this study as a control for the present experiments. Sequencing data of WT mouse testes were obtained from public databases (E-MTAB-6946 < ArrayExpress < BioStudies < EMBL-EBI)