GSM8782043: Gene Expression; Mus musculus; RNA-Seq
RNA extraction was performed per manufacturer's instructions (Single cell 3' v2 protocol, 10x genomics). RNA quality was checked using RIN numbers and nanodrop. Library preparation was performed per manufacturer's instructions (Single cell 3' v2 protocol, 10x genomics). Briefly, FACS sorted CD3+T-cells from homogenized single cell suspension of each cardiac allografts (n=3 G1 and 3 G0 chimeric recipients) were treated with hashtagging barcoded antibodies (10x genomics). Cells were resuspended in master mix and loaded together with partitioning oil and gel beads to generate gel bead-in-emulsions. Poly-A RNA from cell lysate was reverse transcribed, bar-coded, amplified and sequenced.