GSM8561721: Contrl, 2, bulk RNA; Homo sapiens; RNA-Seq
For each sample, total RNA was extracted using TRIzol reagent (Thermo, 15596018CN) and subsequently purified using the RNeasy Mini Kit (Qiagen, 74104), following the manufacturer's instructions. The quality and quantity of the RNA were assessed using a NanoDrop 2000 spectrophotometer, Agilent 2100 Bioanalyzer, and Agilent RNA 6000 Nano Kit (Agilent, 5067-1511). To create the sequencing libraries, the NEB Next Ultra RNA Library Prep Kit for Illumina (NEB, E7770L) was employed. Library clustering was carried out using the HiSeq PE Cluster Kit v4-cBot-HS (Illumina, PE-401-4001), following the manufacturer's recommendations. After cluster generation, the libraries were subjected to sequencing on an Illumina platform, generating 150 bp paired-end reads.