sequence of bacteria and fungi
After DNA extraction and amplification, the amplicon quality was                 visualized via gel electrophoresis. The PCR products were purified with Agencourt                 AMPure XP beads (Beckman Coulter Co., USA) and quantified via a Qubit dsDNA assay                 kit. The concentrations were then adjusted for sequencing. Sequencing was performed                 on an Illumina NovaSeq 6000 with two paired-end read cycles of 299 bases each.                 (Illumina Inc., San Diego, CA; OE Biotech Company; Shanghai,                 China).