pool libraries of  28 clams, North Inlet, SC and Bogue Sound, NC
DNA was extracted from each individual clam using a standard phenol-chloroform-isoamyl alcohol (PCI) extraction protocol. Equivalent quantities of DNA (~100 ng per clam) were used from each clam to create a total of seven DNA pools (1 pool per population). Pooled DNA samples were then used for Illumina library synthesis using an Illumina Truseq Nano DNA library preparation kit and produced libraries were sequenced on a NovaSeq 6000 S4 lane following manufacturers protocols.