GSM8172663: PFA_Retina_rep4; Canis lupus familiaris; RNA-Seq
Total RNA was extracted from FF and PFA-fixed samples using the RNeasy Plus Micro kit and the RNeasy FFPE kit, respectively. For the PFA-fixed samples, specific modifications were made to the manufacturer's protocol. The solubilized tissue sections were incubated with Proteinase K for 18 hours at 56°C for improved RNA extraction efficiency, and a second round of genomic DNA digestion was performed using the DNA-free™ DNA Removal kit post RNA extraction to ensure complete removal of genomic DNA contamination. Sequencing libraries were constructed from RNA extracted from 4 FF and 4 PFA-fixed tissue samples using the SMARTer Stranded Total RNA-Seq kit v3 - Pico Input Mammalian.