GSM8621135: MapID-tRNA-seq, "+AlkB", replicate2, MCF7; Homo sapiens; ncRNA-Seq
RNA samples were extracted from MCF10A, MCF7 and MDA-MB-231 cells using the TRIzol reagent and standard protocol; tRNA 5s and 5.8srRNA were purified by denaturing PAGE. Libraries were constructed using ligation-based library construction approach incoporating the evolved RT, RT-1306, in the current work.