GSM8171844: A498_mRNA_gDNA-seq_Rep2; Homo sapiens; RNA-Seq
Nucleic acid was extracted by SDS gDNA library, mRNA library and mRNA&gDNA library were constructed by extracting whole nucleic acid from cells. RNase A enzyme is used to remove RNA from gDNA library. Genome DNA was removed by digested with DNase I in mRNA library while retained in mRNA&gDNA library as internal reference. mRNA was reverse-transcribed with oligo(dT) as the primer to synthesis the first strand of the complementary DNA (cDNA). To reduce bias and simplify our pipeline, fragmented cDNA without second strand synthesis and gDNA were denatured by heat following library preparation using a high-efficient ssDNA ligation technique, Adaptase from Accel-NGS 1S Plus DNA Library Kit (Swift Accel-NGS).