GSM8444007: scRNA DE (Day 2), Batch_2; Homo sapiens; RNA-Seq
Cells were washed twice with CDM2 medium to remove dead cells and detached using Accutase. Cells were collected and counted using Countess® II Automated Cell Counter. 500,000 cells for each condition were collected in a PBS-0.04%BSA buffer and processed according to the 10X Genomics Single Cell Protocols Cell Preparation Guide (available at https://assets.ctfassets.net/an68im79xiti/56DlUZEsVOWc8sSG42KQis/35cbcf6dcd4b0c0196263ee93815b0ae/CG000053_CellPrepGuide_RevC.pdf). For each cell type, 20,000 cells were loaded per lane on the 10x Genomics Chromium platform, with the goal of capturing 10,000 cells. Cells were processed for cDNA synthesis and library preparation using 10X Genomics Chromium Version 2 chemistry (catalog number 120234) as per the manufacturer's protocol. cDNA libraries were checked for quality on the Agilent 4200 Tape Station platform and their concentration was quantified by KAPA qPCR.