GSM6996239: MCF7-dCas9-KRAB_Rep1__; Homo sapiens; ATAC-seq
Cells were collected, washed once with PBS and followed by ATAC-seq library construction. 50,000 cells were collected for ATAC-seq library construction. 100 nM of Tn5 were used during the transposition reaction followed by the column cleanup. We used KAPA HiFi polymerase (Roche) for PCR amplification with 7 cycles and a 1.4X SPRI beads cleanup.