GSM8002031: E14.5dKO, scRNA-seq; Mus musculus; RNA-Seq
Cartilage anlagen were isolated from the hindlimbs of E13.5 or E14.5 embryos, and cleaned of soft tissues by rolling on Kimwipe paper. Presumptive knee joint regions were excised under a dissecting microscope (Leica) and digested in 4 mg/ml collagenase D (Sigma-Aldrich, Cat# 11088858001) on an orbital shaker at 120 rpm, 37℃ for 20 mins. Libraries were constructed with the Single Cell 3' Reagent Kits v3.1 (10x Genomics), and sequenced with a NovaSeq sequencer at the Center for Applied Genomics of The Children's Hospital of Philadelphia. Libraries were constructed with the Single Cell 3' Reagent Kits v3.1 (10x Genomics), and sequenced with a NovaSeq sequencer at the Center for Applied Genomics of The Children's Hospital of Philadelphia.