RNA-seq of mixed-age pool of Leptopilina malgretoutensis
Total RNA was extracted from a flash-frozen, mixed stage pool of 10 wasps (adults, pupae, larvae) using the Monarch Total RNA Miniprep Kit (New England Biolabs) including the DNase treatment step. An RNA-Seq library was prepared with the Illumina TruSeq Stranded mRNA Library Prep Kit, including rRNA removal with oligo-dT beads, and sequenced on a NovaSeq with S4 chemistry.