GSM8172473: 30y_F3; Homo sapiens; RNA-Seq
Fresh peripheral blood collected in EDTA tubes was mixed with three volumes of red blood cell lysis buffer (Solarbio, R1010) and gently vortexed for 15 minutes on ice to lyse the red blood cells. After centrifugation at 4°C and 450 × g for 10 minutes, the leukocytes were separated. Total RNA was extracted from the leukocytes using TRIzol reagent (Thermo Fisher, 15596018). Whole-transcriptome sequencing library preparation and sequencing were performed by Lianchuan Biotechnology Co., Ltd. (Hangzhou, China).