GSM7469236: DaGS-Atx2RNAi-Rep3; Drosophila melanogaster; RNA-Seq
Total RNA was extracted from brains using trizol/chloroform and Zymo RNA clean and concentrator kit-5 (R1015). The RNA-seq libraries from brains were prepared using the Tru-seq stranded mRNA library prep. Library preparation and sequencing was done by Admera Health, and sequenced using illumina HiSeq with 40M paired end reads (2x150bp). Three biological replicates were done for each experimental timepoint, condition, and genotype. The RNA-seq libraries from brains were prepared using the Tru-seq stranded mRNA library prep. Both m6A-IP and RNa-seq were sequenced with 40M paired end reads (2x150bp).