GSM8173065: AC16 Clone 9B4, IKK2-EE; Homo sapiens; RNA-Seq
iPSCs, AC16-CAR cells, and unperturbed AC16 clones: lysed with buffer RLT and mRNA extracted with the RNeasy Mini Kit. Perturbed AC16 clones: lysed with buffer RLT and mRNA extracted with the RNeasy Plus Mini Kit. iPSCs, AC16-CAR cells, and unperturbed AC16 clones: prepared with the TruSeq stranded mRNA library prep kit. Perturbed AC16 clones: strand-specific sequencing libraries, after poly(A) pulldown, were prepared by Novogene.