GSM8619585: AR003, Rspo3 KO HSC_3; Mus musculus; RNA-Seq
Liver tissue was homogenized in a Tissuelyser in Trizol and purified using chloroform, followed by isolation of total RNA using RNA isolation kits (QIAGEN, Roche or Sigma-Aldrich). Total RNA from cells was isolated directly using RNA isolation kits. Libraries were constructed using Illumina TruSeq RNA Preparation Kit according to the manufacturer's instructions.