UCE sequence data of adult Corynura_chloris
DNA for this sample was extracted from whole specimen or leg tissue using a standard CTAB phenol/chloroform protocol. We quantified DNA extracts using a Qubit 3.0 fluormeter (Thermo Fisher Scientific, Inc.) and assessed fragmentation by running 2 ul on a 1.5% sodium borate gel. We sheared approx. 200 ng of DNA to a targeted fragment size of 500-600 bp using a Q800R sonicator (Qsonica, LLC.). After DNA extraction and shearing, samples were processed as dual-indexed Illumina libraries using TruSeq adapters (Faircloth and Glenn 2012). Enrichment was carried out using the Hym-v2 bait set (ant-specific version), We followed the enrichment procedure from Faircloth et al. (2015), with the modifications from Blaimer et al. (2016b, 2016a). Following enrichment, we measured DNA quantity with a Qubit fluorometer (model 3.0, Thermo Fisher Scientific, Inc.) and measured the fragment distribution using a TapeStation (Agilent 2200 TapeStation) and a D1000 HS screen tape (Agilent Technologies, Inc.). We pooled at equimolar concentrations and size selected for a range of 250-800 bp with a Blue Pippin device (Sage Science, Inc.). Sequencing was carried out at the Cornell Biotechnology Resource Center (http://www.biotech.cornell.edu/biotechnology-resource-center-brc), using an Illumina HiSeq 2500 platform with 150 bp paired end reads. The barcode combination for this sample is:  i5: CACAGACT and i7: GCGCATAT.