GSM8435128: control saline, airway epithelium, adult; Mus musculus; RNA-Seq
Following perfusion, lung were inflated with dissociation buffer (Collagenase/Dispase/DNAseI) and incubated at 37C for 30min in a shaking incubator. After washes, red blood cells were removed through RBC lysis buffer. Epithelial cells were isolated by magnetic bead sorting through removing CD45+ and CD31+ cells. Remaining cells were stained with Epcam (APC) antibody and DAPI was used to sort for live cells. tdTomato+, Epcam+ cells were sorted to enrich for PNEC population. For scRNA, single cell libraries were generated from sorted epithelial cells by using Chromium Single Cell 3' v3 kit (10X Genomics).