GSM7889077: A17, root, deficit, rep1; Medicago truncatula; miRNA-Seq
Total RNAs were extracted using the miRNeasy mini kit (Qiagen). Libraries were prepared using the NEXTFLEX Small RNA-seq kit v3 (PerkinElmer) following the manufacturer's instructions. The resulting barcoded library was size-selected (137-187 bp) using a Pippin HT (SAGE Science) with a 3 % agarose cassette (#HTG3004).