GSM8343196: set3.heso1_rep3; Arabidopsis thaliana; RNA-Seq
Inflorescences and rosettes for northern blots, western blots and sequencing libraries were collected and snap-frozen in liquid nitrogen. Total RNA from inflorescences was extracted with TRI Reagent (#T9424, Sigma-Aldrich Denmark A/S) according to the manufacturer's instructions. The RNA was once again resuspended and purified with phenol:chloroform:isoamyl alcohol. The RNA was dissolved in nuclease-free water and RNA integrity was assessed on a Bioanalyser RNA Nano Chip (Agilent Technologies). 10 μg of total inflorescence RNA was used as input in each library. Twenty pmol of a 3'