Total RNA-Seq MHV68 Infection Models
NIH 3T3 cells were infected with MHV68 H2B-YFP at an MOI of 5 PFU per cell. RNA was collected from infected samples at 6 hours post infection. Total RNA was isolated from cells using the Direct-zol RNA MiniPrep Kit. ERCC spike-in controls were added. RNA was ribominus selected and directional cDNA libraries were generated using either Stranded Total RNA Prep with Ribo-Zero Plus (Illumina # 20040525) or TruSeq Stranded Total RNA Ribo-Zero Gold (Illumina #RS-122-2303). Sequencing was performed using the Illumina NovaSeq SP platform to generate 150 bp PE reads.