GSM7174210: Patient R3388 prot; Homo sapiens; RNA-Seq
NA Library was prepared using Chromium Single Cell 3ʹ version CG000317 rev B protocol (10x Genomics). Cellular mRNA and antibody-derived oligos were reverse-transcribed and indexed with a shared cellular barcode. Indexed cDNAs were then pooled and amplified by PCR according to the Chromium Single Cell 3' version CG000317 rev B protocol (10x Genomics) and with specific primers amplifying the antibody-derived tags. SPRI bead size selection was performed in order to separate both the mRNA-derived cDNA and the antibody-derived tagged cDNAs. For the mRNA derived cDNA library preparation, we further proceeded according to the manufacturer's instructions for Single Cell 3' version CG000317 rev B protocol (10x Genomics). Libraries were equimolar pooled to obtain at least 20,000 read pairs per cell and 5,000 read pairs for feature barcode part after sequencing on Illumina Novaseq 6000 (100 cycles cartridge). OTHER : CITE-seq