RNA-Seq of islet organoids-S5D3-FGF2 1
Cells generated with the present protocol at S5D3 and S7D6 were harvested and lysed in Trizol reagent and snap frozen in liquid nitrogen. RNA was extracted using the conventional phenol-chloroform RNA extraction method. The library was prepared according to a published protocol. The library was then sequenced on an MGISEQ-2000RS FAST instrument using MGISEQ-2000RS(PE100) kits.