GSM7117347: Col6a2_5W_F_Rep1; Mus musculus; RNA-Seq
The RNA was isolated and purified as previously described (PMID: 20516177). RNA quality was analyzed using a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). Samples that had a RIN/RINe ≥ 8 were used for RNA-Seq library construction. Libraries were prepared according to the manufactirer's protocol for the Illumina TruSeq RNA Sample Prep Kit.  Starting amount of total RNA used was 150ng. The cDNA produced was fragmented using a Covaris E210 and amplified using 12-15 cycles. Paired-end sequencing of the libraries for 150bp per read was performed using the Illumina MiSeq platform with a target depth of 100 million reads per library. Sequences returned were processed via RTA and deplexed using CASAVA (http://www.illumina.com/software).