GSM8442863: N/TERT-2G keratinocytes, CXCL14 knockdown, rep2; Homo sapiens; RNA-Seq
Total RNAs were isolated using the Quick-RNA™ Miniprep Kit (Zymo Research) following the manufacturer's protocol. Messenger RNA was purified from total RNA using poly-T oligo-attachedmagnetic beads. After fragmentation, the first strand cDNA was synthesizedusing random hexamer primers, followed by the second strand cDNAsynthesis using either dUTP for directional library or dTTP for non-directional library