GSM8132044: Smarca5_cntrl; Mus musculus; OTHER
B cells were sorted into FACS wash buffer, and nuclei were isolated using the Chromium Nuclei Isolation Kit (10x Genomics) according to the manufacturer's instructions. Nuclei were counted using trypan blue and hemocytometer, and imaged at 40X magnification to assess their quality. Nuclei were diluted in nuclei buffer supplemented with 1 mM DTT and 1 U/µl RNAse inhibitor to a final concentration of 3700 nuclei/ul. Nuclei were processed with the Chromium Next GEM Single Cell Multiome ATAC + Gene Expression kit (10x Genomics) according to the manufacturer's protocol. Final libraries were quantified by qPCR with the NEB-next Library Quant Kit (New England Biolabs) and with Qubit and TapeStation. Sequencing was done with a Nova-Seq 6000 (Illumina) using two SP100 cycle kits (for the ATAC-seq library and for the gene expression library), allocating 25,000 read pairs per nucleus for the ATAC-seq library and 20,000 read pairs per nucleus for gene expression libraries.