GSM8619541: TAL1 rep1, Day8; Homo sapiens; OTHER
1 × 105 HSPC cells were harvested freshly. The cells were enriched by ConA Beads and antibody. Then the beads were washed for 3 times using antibody buffer and then incubated with proteinA/G-Tn5 transposome for 1 h and washed for 3 times by ChiTaq buffer. Cells were resuspended in 50 µL Tagmentation buffer (10 mM MgCl2 in ChiTaq Buffer) and incubated at 37 °C for 1 h. The incubation was stopped by adding 10 µL 10% SDS at 55 °C for 10 min. The DNA fragments were extracted by Tagment DNA extract beads and amplified using 5× AmpliMix. Then the DNA was re-extracted by DNA clean beads for sequencing. cDNA libraries were prepared as described (Nat Commun. 2019 Apr 29;10(1):1930) and sequenced with Novaseq-PE250 platform.