Paired-end Illumina shotgun sequencing of live oyster shellstock preenrichment
Pre-enrichment culture from live oyster shellstock; 10 mL pelleted and resuspended in Zymo DNA/RNA Shield. DNA extraction using Zymo Quick-HMW kit with RNAseA treatment. NEBNext UltraII DNA library prep; paired-end (2x150) sequencing using NovaSeq 6000.