MCF7_WS8 CELLS WITH T6I-291A & E2 SAMPLE 1_1
mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library. End repair, A-tailing, adapter ligation, size selection, +/- USER enzyme direction, amplification, and purification was performed. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries were pooled and sequenced on Illumina platforms. Clustering and sequencing was performed and paired-end reads were generated.