Paired-end Illumina shotgun sequencing of frozen shrimp sample preenrichment
Pre-enrichment culture from frozen shrimp sample; 10 mL pelleted and resuspended in Zymo DNA/RNA Shield. DNA extraction using Zymo Quick-HMW kit with RNAseA treatment. NEBNext UltraII DNA library prep; paired-end (2x150) sequencing using NovaSeq 6000.