RNA-seq of mouse heart: sham02
RNA was extrated from mouse heart and purificated. mRNA was Isolated and fragmented. Then synthesis cDNA. After that through PCR to amplify the product.Single-stranded circle DNA molecules are replicated via rolling cycle amplification, and a DNA nanoball (DNB) which contain multiple copies of DNA is generated. Sufficient quality DNBs are then loaded into patterned nanoarrays using high-intensity DNA nanochip technique and sequenced through combinatorial Probe-Anchor Synthesis (cPAS).