GSM8476941: Chip with H1.2, control siRNA; Homo sapiens; ChIP-Seq
The fixed cells were suspended in lysis buffer and extensively sonicated. The length of DNA extracted from the cell extracts were confirmed to be between 200 and 500 base pairs. The purified DNA (50 ng) was used for library construction using ChIP-Seq library preparation kit (ThruPLEX DNA-Seq kit, Takara Bio) according to the manufacturers' instruction