GSM7467236: Tmem119-creERT2-cGaswt/wt microglia mouse 3, scRNAseq; Mus musculus; RNA-Seq
Nuclei from mouse brain were extracted by homogenizing mouse brain tissues in Nuclei EZ Lysis Buffer using a douncer. Nuclei were labelled with DAPI and sorted for sequencing. To enrich microglial cells, in addition to DAPI staining, nuclei were stained with anti-RBFOX3/NeuN-647. Sorted nuclei were then loaded into a Chromium Single Cell Controller. ChromiumSingle Cell 3' Library & Gel Bead Kit v3.1