DNA-Seq of homo sapiens
Genomic DNA samples were quantified using the Qubit Fluorometer 3.0 and approximately 25ng of genomic DNA was used for sample assessment. Automated dual indexed libraries were constructed with ~300-500ng of genomic DNA utilizing the KAPA Hyper Prep Library Kit (Roche Diagnostics, Cat # 7962363001) on the SciCloneG3 NGS NGS instrument (Perkin Elmer) targeting 250bp inserts.  Libraries were pooled (500ng each) pre-capture generating  1-2g library pools.  Each library pool was hybridized overnight with the xGen Exome Hyb Panel v1 (IDT, Cat # 1056115) and 1 ul of the diluted TERT promoter probe. The molarity of each library capture pool was accurately determined through qPCR utilizing the KAPA library Quantification Kit (Roche Diagnostics, cat # 7959478001/ 7960085001) to produce cluster counts appropriate for the Illumina NovaSeq6000 instrument.  Normalized library pools were sequenced on a NovaSeq6000 S4 Flow Cell using the XP workflow and a PE 151 cycle sequencing recipe according to manufacturer protocol targeting 24Gb per library.