GSM7890070: Clone_G Untreated S2; Homo sapiens; RNA-Seq
1.5×10^5 MEFs were plated on a 6-well plate a day before the extraction. Total RNA was isolated using the NucleoSpin RNA II kit (Macherey-Nagel). The amount of RNA was determined using a NanoDrop spectrophotometer (Thermo Fisher Scientific). SMART-Seq mRNA library prepartion following manufactures's intructions (TANAKA).