GSM8002286: D6TAZ biol rep2; Mus musculus; ATAC-seq
Nuclear suspensions obtained from 100,000 cells were treated with assembled Tn5 transposase for 30 min at 37°C. Tagmented DNA was cleaned up with a MinElute PCR Purification Kit (28006, Qiagen) before library preparation. Library preparation for ATAC-seq was performed with the use of an ATAC-seq kit (AT53150, Active Motif) and the Omni-ATAC protocol (Corces et al. 2017)