GSM8131856: T6_ID; Homo sapiens; ATAC-seq
ATAC-seq was done as previously described (Buenrostro et al., 2013). Briefly, 50000 FACS sorted CD3+ T cells were washed in 1xPBS, re-suspended in 50 ul of lysis buffer (10 mM Tris pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% NP40,) and spun at 500g for 10 min at 4 °C to collect nuclei. Nuclei were subsequently re-suspended in 50 µ