RNA-Seq of chicken
In this project, mRNA with polyA structure in total RNA was enriched by Oligo(dT) magnetic beads, and RNA was broken into fragments of about 300bp in length by ion interruption. The fragment length of 300bp was selected to synthesize the first cDNA strand using RNA as template, and the second cDNA strand was synthesized using the first cDNA strand as template using 9-base random primers and reverse transcriptase.