Root Transcriptome Analysis of Wild-Type Drimia
Roots were flash-frozen in liquid nitrogen, and total RNA was isolated using the RNeasy Plant Mini Kit (Qiagen), with DNase treatment (TaKaRa). RNA quality was checked using TapeStation (Agilent), resulting in a 28S/18S ratio of 2.2 and a RIN of 7.0. The mRNA library was prepared using the Qiagen mRNA Library Kit, including RNA fragmentation, cDNA synthesis, and adapter ligation. Sequencing was performed on the Illumina NovaSeq 6000 platform to achieve high-depth coverage for root transcriptome analysis.