GSM8530765: WT-1; Mus musculus; RNA-Seq
we conducted a comparative RNA sequencing (RNA-seq) analysis on primary murine hematopoietic cells transduced with either empty vector or RUNX1-RARA. Total RNA was isolated and purified using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer's procedure. The RNA amount and purity of each sample was quantified using NanoDrop ND-1000 (NanoDrop, Wilmington, DE, USA). The RNA integrity was assessed by Bioanalyzer 2100 (Agilent, CA, USA) with RIN number >7.0, and confirmed by electrophoresis with denaturing agarose gel. Poly (A) RNA is purified from 50μg total RNA using Dynabeads Oligo (dT)25-61005 (Thermo Fisher, CA, USA) using two rounds of purification. (m6A-seq) Libraries were generated using the NR605-02 V8 RNA-seq Library Prep Kit for Illumina and sequenced with Illumina NovaSeq6000.(RNA-seq)