snRNA-seq analysis of Nestin-cre;Tox3f/f postnatal day 1 mouse cerebellum
Single nuclei were generated from 12 snap-frozen cerebella from each condition using the SingulatorTM 100 system (S2Genomics, Livermore, CA), following the regular nuclei isolation protocol. Single-nucleus RNA-seq libraries were generated using the Chromium Next GEM Single Cell 3 v.3.1 according to the manufacturers protocol (10x Genomics), targeting 10,000 nuclei per sample.