GSM7468686: HONE1 cells, input; Homo sapiens; ChIP-Seq
ChIP assay was performed using a pierceTM Magnetic ChIP Kit (#26157, ThermoFisher) according to its protocol. Briefly,  cells were crosslinked with 1% formaldehyde for 10 min and quenched with 125 mM glycine at room temperature. Then, the cell nuclei were isolated, lysed, and sonicated to yield 200-1000 bp chromatin fragments. The chromatin fragments were immunoprecipitated with 3 ul anti-HA antibody (#ab9110, Abcam) or anti-rabbit IgG antibody (#26157, ThermoFisher) overnight at 4 °C and then immobilized on protein A/G magnetic beads for 2 h at 4 °C. After washing, the enriched DNA was eluted, de-crosslinked, purified, and then subjected to ChIP-seq or qPCR analyses. The obtained ChIP-DNA and input DNA fragmentsby ChIP assay were firstly end-repaired and A-tailed using the NEBNext End Repair/dA-Tailing Module (#E7442, NEB, USA) and then ligated adaptor using the NEBNext Ultra Ligation Module (#E7445, NEB). The DNA libraries were amplified for 15 cycles for obtaining more material