GSM7890593: FF; Homo sapiens; RNA-Seq
RNA was extracted from PBMCs using the RNeasy Mini Kit (Qiagen, Germany), retro-transcribed in cDNA and quantified using the Qubit 4.0 fluorimetric Assay (Thermo Fisher Scientific) and sample integrity, based on the DIN (DNA integrity number), was assessed using a Genomic DNA ScreenTape assay on TapeStation 4200 (Agilent Technologies) Genomic DNA was Libraries were prepared from 100 ng of total DNA using the NEGEDIA Cancer Haemo Exome sequencing service (Next Generation Diagnostic srl)