GSM8404564: Chronic Sample4 HTO; Mus musculus; RNA-Seq
From splenocytes, LCMV GP66-77 (PE) tetramer positive, CD44(hi), CD4+T cells were isolated and enriched; First, CD4+T cells were negatively selected (Stemcell), followed by anti-PE positive selection of LCMV GP66-77 (PE) tetramer+ cells (Stemcell). Purified cells were barcoded (Biolegend) to identify the affiliated, individual animal for each cell. Cells were pooled accordingly and LCMV GP66-77(PE) tetramer+, CD44hi, CD4+T cells were then FACS sorted and loaded onto a Chromium NextGEM Chip K (10xGenomics). Two channels of the chip were used for each condition (neonat.; adult) following the manufacturer's instructions. The library was constructed according the manufacturers instructions of the Chromium Next GEM Single Cell 5' v2 Dual Index Kit (10xGenomics)