GSM8128577: EC3, replicate A, Embryonal carcinoma cells derived from a murine malignant teratocarcinoma; Mus musculus; RNA-Seq
Total RNA was extracted from cells using RNA Stat-60 according to the manufacturer's protocol, with the addition of GlycoBlue Coprecipitant (Invitrogen) immediately prior to isopropanol precipitation. RNA sample quality was determined by spectrophotometry (Nanodrop) to determine concentration and chemical purity and with a Fragment Analyzer (Advanced Analytical) to determine RNA integrity. Directional RNA-seq libraries were prepared from 1ug total RNA using the NEBNext Directional Ultra II RNA Library Prep Kit for Illumina (New England Biolabs), with initial polyA+ isolation.