GSM8529202: Young, male, biological replicate 1, cebpb CUT&RUN; Mus musculus; OTHER
CUT&RUN was performed using the CUT&RUN Assay Kit (cell signaling technology, 86652) following the manufacturer's instructions. Briefly, 500,000 microglia isolated from one biological replicate were used for each reaction. The cells and concanavalin A-coated magnetic beads slurry were incubated with antibodies overnight at 4 °C. DNA was purified from enriched chromatin samples using NEB Monarch® DNA Cleanup Kit(NEB, T1130) following the manufacturer's instructions. Libraries were constructed using all CUT&RUN-enriched DNA with the NEBNext® Ultra™ II DNA Library Prep Kit (NEB, E7645S).The libraries were size-selected and purified with SPRIselect (Beckman, Coulter B23318), and quality checked on Agilent Bioanalyzer® High Sensitivity DNA chip (Agilent Technologies,5067-4626).  Equimolar amounts of each library were combined, and the pooled library was further quantified using a NEBNext Library Quant Kit (New England Biolabs).