GSM8405711: ChIP_ONECUT1_PP1_1; Homo sapiens; ChIP-Seq
Cells were crosslinked by formaldehyde and lysated by SDS lysis buffer. Sonicated nuclei were incubated with antibody overnight and then pulled down by dynabeads. After washing, decrosslinking was performed to isolate DNA. Library was prepared using NEBNext Ultra II DNA Library Prep Kit (NEB) according to manufacturer's protocol.