ChIP-seq H3K27ac of Xenopus tropicalis: Osteoblasts tadpoles
Crosslinking was achieved by formaldehyde fixation, followed by glycine quenching. Subsequent steps involved cell homogenization, nucleus purification, and sonication for chromatin fragmentation. Sample preparation was carried out using the Magna ChIP TM A kit, and for the immunoprecipitation, antibody H3K27ac (Abcam, Ab4729) was employed.