GSM7887998: Female_midgut_control_2; Drosophila melanogaster; RNA-Seq
Total RNA was extracted using the Rneasy Mini kit (Qiagen). 200ng were used for the construction of the sequencing libraries. PolyA enrichment was performed using NEBNext® Poly(A) mRNA Magnetic Isolation module, and NGS RNASeq libraries made using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® according to manufacturer's instructions For each sample, a minimum of 40 million Paired End 100bp reads were generated on an Illumina HiSeq2500 with single 6bp indexing.