GSM8561630: Head, Control, Input, rep1; Drosophila melanogaster; ChIP-Seq
Chromatin preparations were carried out as described in Chanas et al. 2004 and Tamberg et al. 2020. Indexed Illumina libraries were prepared using Ovation Rapid DR Multiplex System 1-96 (NuGEN) according to the manual. Libraries were amplified for 10 cycles using MyTaq (Bioline) and standard Illumina primers. Illumina libraries were pooled and size selection was done by preparative gel electrophoresis selecting a range between 300 and 500bp. Final library purification step and quality control of DNA libraries via Fragmentanalyzer (Agilent) and Qubit. Sequencing was done on an Illumina NextSeq 500/550 - 1x75 bp read length (Illumina) -followed manufacturer's instructions.