GSM8620453: C17,cherry; Homo sapiens; Hi-C
Hi-C template preparation was performed as described in Rao et al (2014). In brief, ten million cells per sample were crosslinked with 2% formaldehyde followed by quenching by glycine at final concentration of 0.2 M. Four-cutter restriction enzyme DpnII was used for in situ digestion. Digested DNA were repaired with biotin-14–dATP in a Klenow end-filling reaction. End-repaired, ligated, and reverse-crosslinked DNA was subsequently purified using isopropanol and magnetic beads. Purified DNA was sheared to 300-500 bp with Covaris and subsequently size-selected by AMPure XP. Appropriately-sized ligation fragments marked by biotin were pulled down with MyOne Streptavidin C1 DynaBeads (Invitrogen) and prepped for Illumina sequencing. Hi-C library preparation was performed as described in Rao et al (2014). In brief, appropriately-sized ligation fragments marked by biotin were pulled down with MyOne Streptavidin C1 DynaBeads (Invitrogen) and prepped for Illumina sequencing.