GSM6996271: Lgr5+ intestinal stem cells, Allo recipient, replicate 4; Mus musculus; ATAC-seq
We FACS sorted 50000 Lgr5+ISCs each independently biology samples, which were pooled 3-4 mice together from Lgr5-GFP allogeneic or syngeneic recipients seven days following BMT. To generate the ATAC-Seq library, we used the Omni-ATAC-Seq protocol  We used the Omni-ATAC-Seq protocol (Corces et al., 2017). The final libraries were cleaned using Qiagen MinElute columns and AMPure XP beads before quantitation with the Qubit HS dsDNA kit, and quality assessment on a TapeStation HS D1000 kit. The libraries were pooled and quantitated by qPCR using KAPA's Illumina Library quantitation kit before PE-150 sequencing on a NovaSeq6000 S4 shared flow cell at the Advanced Genomics Core.