Exploring the transcriptome of immature stages of Ornithodoros hermsi, the soft-tick vector of Borrelia hermsii
Total RNA was extracted from whole body Ornithodoros hermsi second stage nymphs. Ticks were grouped in three biological replicates (n 5 individual per replicate) and are composed of nymphs fed on neonate mice at 12h-post detachment (Oh-12h). RNA extraction was performed with the PureLink RNA Mini Kit (Invitrogen) according to manufacturer`s specifications and analyzed with an Agilent High Sensitivity RNA ScreenTape System in an TapeStation 4150 (Agilent). The library was constructed using the NEBNextUltraTM II (Directional) RNA with polyA selection library prep kit and sequencing was performed in an Illumina Novaseq 6000 DNA sequencer.