GSM8002068: shMETTL3 IP; Homo sapiens; RIP-Seq
Total RNAs were extracted utilizing RNA-easy Isolation Reagent (Vazyme, China) and treated with DNase I (Roche, Switzerland), which were then fragmented into 100-150 nt fragments with RNA Fragmentation Reagent (Invitrogen, USA). The meRIP-seq libraries were used the SMARTer Stranded Total RNA-Seq Kit v2-Pico Input Mammalian (Takara, Japan). 10 ng of fragmented RNA from each sample was used as starting material for Input and IP samples. Amplification of libraries were performed with 14 cycles on the thermocycler. The samples were sequenced with paired-end on Illumina NovaSeq 6000.