GSM8477166: i33-1805-24hr_S33; Homo sapiens; RNA-Seq
Total RNA was extracted from cell lines and tumour samples from treated mice using TRIzol reagent, followed by purification with the Direct-zol RNA Miniprep Kit (Zymo Research), which includes an on-column DNase I digestion step to remove genomic DNA Poly(A) RNA was isolated from the total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). First-strand cDNA libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs)