RNA-Seq of Callosobruchus maculatus: temperature 3
Individual larvae samples were homogenised mechanically, and total RNA was extracted using a standard Trizol-chloroform procedure. RNA pellets were then reconstituted in 30uL RNase-free DNase water and RNA concentration and quality were determined using Qubit fluorometry. Messenger RNA (mRNA) library preparation and sequencing was performed by Novogene Co. Ltd. Specifically, the libraries were sequenced with the NovaSeq 6000 PE150 platform (Illumina) to produce 20 million 150-bp paired-end reads.