GSM8078999: MIN6 stable cells with miR-146a-5p inhibited, without proinflammatory cytokines - replicate3 [P67-ZIP]; Mus musculus; RNA-Seq
Total RNA was isolated using miRNeasy mini kit (217004; Qiagen). RNA concentration was measured using a NanoDrop 2000 (ThermoFisher Scientific). One hundred nanograms of total RNA was used for library preparation.Library amplification was performed following the KAPA mRNA Hyper Prep Kit Technical Data Sheet, KR1352 – v4.17 (Roche Corporate)