GSM8619582: BGLT3 sh2, Day14; Homo sapiens; RNA-Seq
The harvested viral particles were added into the day 4 CD34+ plus with 8 µg/ml polybrene for 12 hours. Then the cells were replaced with fresh complete medium and subjected to the following experiments. The cells were collected and RNA was harvested using Trizol reagent. RNA libraries were prepared for sequencing using standard Illumina protocols.