GSM7888027: CBir_undivided cells, ATAC-seq; Mus musculus; ATAC-seq
Naïve CD4+ CBir1 T cells were enriched from the spleen of CBir1 transgenic mice, using Miltenyi kit 130-104-453, stained with CellTraceTM Violet and injected into four WT C57BL/6J recipient mice. Starting on the following day, recipient mice were treated with 2% dextran sulfate sodium (DSS) in drinking water for 7 days, followed by normal drinking water, to induce intestinal barrier disruption. Recipient mice received 1 mg/kg of body weight FTY720 on days 7, 10 and 13 after the beginning of DSS administration, to retain cells in the lymph nodes. On day 14 after the beginning of DSS treatment, we isolated cells from the colon-draining mesenteric lymph nodes (cMLNs) of recipient mice, and sorted them into 3 fractions: 1) undivided (CTVhi) CBir1 T cells, 2) divided (CTVdim) CBir1 T cells, and 3) total non-CBir1 endogenous CD4+ T cells (polyclonal Th cells). Libraries were prepared using the method described in Buenrostro et al. (Nature Methods 2013)