GSM6997878: d15; Mus musculus; RNA-Seq
On day 0 (unstimulated), 8 and 15 post-induction (equivalent to 0, 4 and 7 doses), skin samples (3-4 mice pooled per sample) were excised and digested in 0.3% collagenase-A, 0.25mg/ml Dispase-II, 10U/ml DNaseI and 2%FBS in RPMI for one hour to obtain single cell suspension. FcR was blocked with 2.4G2 before incubating with anti-CD45-magnetic beads (Biolegend, #480028). Magnetically isolated cells were subjected to sequencing profiling. Library was prepared with Chromium Single Cell 3' Reagent Kits (v3.1 Chemistry; 10x Genomics). The 3' gene expression libraries with paired-end Read 1 (containing 16-bp barcode and 12-bp UMI), Read 2 (91-bp insert) and i7 index (8-bp) were analysed by Illumina HiSeq X.