GSM8076531: MSC GEX; Homo sapiens; RNA-Seq
Cells were then lysed with 0.1X lysis buffer for 4 minutes to isolate nuclei, based on the protocol of Demonstrated Protocol Nuclei Isolation Complex Sample ATAC GEX Se-quencing, CG000375 RevB (10X Genomics, Inc). Following the Chromium NextGEM Multiome ATAC GEX User Guide, CG000338_RevB (10X Genomics, Inc), tagmentation of nuclei preparation was first performed. Thereafter, briefly, along with the single cell multiome gel beads and partition oil, the single nuclei master mixture containing tagmented single nuclei suspension was transferred onto a Next GEM Chip J, and the chip loaded to the Chromium Controller for GEM generation and barcoding, followed by pre-amplification PCR, ATAC library preparation, cDNA synthesis and cDNA library preparation. single cell multiome