TTF1 control of LncRNA synthesis and cell growth delineates a tumour suppressor pathway acting directly on the  ribosomal RNA Genes
TTF1 control of LncRNA synthesis and cell growth delineates a tumour suppressor pathway acting directly on the  ribosomal RNA Genes
TTF1 conditional Clone C8 was cultured for >7days with 100ng.ml-1 doxycycline (Dox100), cells were then either harvested or cultures continued for a further 3, 5 or 7 days without doxycycline (Dox0). Cultures were then either harvested or 100ng.ml-1 doxycycline readded and cultures maintained for a further 3, 5, and 7 days (Dox100-Readdition). Total RNA was extracted from these cultures and from parallel cultures of NIH3T3 using Trizol and GeneJet RNA micro kit (ThermoFisher Scientific). RNA sequencing libraries were created using the NEBNext mRNA stranded protocol (New England Biolabs), (McGill University and Genome Quebec Innovation Centre).