GSM8498701: Het_5; Mus musculus; RNA-Seq
Total RNA was extracted from 20 mg mouse skeletal muscle using TRIsure-chloroform-ethanol (Bioline) method followed by in-column DNA digestion (Qiagen). RNA concentrations were measured with Agilent TapeStation and Nanodrop, RNA integrity was checked on a gel with Agilent TapeStation. The RNA-seq libraries were made with NEBNext Ultra II Directional (PolyA) kit and sequenced with NextSeq High Output 1x75bp (Illumina) at Biomedicum Functional Genomics Unit (FuGU).