GSM8264912: Mononuclear cells, diagnosis, TMD, 273; Homo sapiens; Hi-C
We have employed the Omni-C kit from Dovetail Genomics for DNase-based Hi-C. 1 x 10^6 MNCs were fixed in DSG, followed by formaldehyde and fixed cells were treated with DNaseI. Next, the chromatin ends were polished and biotin-tagged bridges were used to create chimeric molecules. The library preparation kit used in the Dovetail Omni-C protocol is called the  "Dovetail Library Kit." This kit is specifically designed to prepare sequencing libraries from the DNA fragments generated during the Hi-C experiment using the Dovetail Omni-C technology. It includes steps such as end repair, adapter ligation, and PCR amplification to generate libraries.