GSM8265087: Lib28_INPUT; Shigella flexneri; Tn-Seq
gDNA isolation was performed using the GenElute Bacterial gDNA kit (Sigma; NA2110-1KT). 1.5ug of gDNA was cleaned with Ampure XP beads to remove EDTA. 500ng of cleaned gDNA was used as input for the sequencing library preprarations. TraDIS library (Barquist et al., 2016 Bioinformatics)