GSM8404738: CJ positive_2; Rattus norvegicus; RNA-Seq
Between 72-92 ng of RNA per sample was subjected to rRNA depletion via the NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat; NEB #E7400) using the manufacturer's protocol except that a Monarch® RNA Cleanup Kit (10 µg; NEB# T2030) was used to purify the rRNA-depleted RNA The rRNA-depleted RNA was converted into Illumina-compatible DNA libraries via the NEBNext® Ultra™ II Directional RNA Library Prep with Sample Purification Beads kit (NEB# E7765) using the manufacturer's protocol for use with rRNA-depleted RNA and NEBNext® Multiplex Oligos for Illumina® (NEB# E6448). Samples were pooled and sequenced 2x150 nt paired-end on an Illumina® Novaseq 6000 instrument