GSM8498729: CREM fl/fl and CREM fl/fl CRE-ER same sex littermates; WT, rep 3; Mus musculus; RNA-Seq
Total RNA was extracted from cecal tissue snips using a Qiagen RNeasy Mini Kit followed by Invitrogen Turbo DNA-Free Kit DNase treatment according the manufacturer's instruction Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.