GSM8591198: R2; Mus musculus; RNA-Seq
A total of 10^7 cells were prepared for cell lysis. Then, the cell medium was discarded, and the cells were washed twice with ice-cold PBS containing 100 μg/mL cycloheximide (Adooq, 66-81-9). EpiTM RiboRNA Depletion Kit (Human/Mouse/Rat) (Epibiotek, R1805) was used for rRNA depletion. mRNA libraries were constructed by using Illumina TrueSeq mRNA sample prepa ration kit (Cat. No. RS-122-2101) according to the manufacturer's instructions.