RNA-seq of endotheliocytes from rat brain blood vessels after incubation with gamma-Fe2O3 nanoparticles
RNA-seq libraries were prepared using MGIEasy rRNA Depletion Kit (MGI tech, China) for rRNA depletion and MGIEasy RNA Library Prep Set (MGI tech, China) for further modifications according to the manufacturers protocol. Sample purity and fragment size distribution were estimated by 1.5% agarose gel electrophoresis. Average fragment size was about 330 bp (insert size 200-250 bp). Final dsDNA concentration was evaluated on the fluorimeter QFX (DeNovix, USA) using reagent kit QuDye dsDNA HS (Lumiprobe, Russia). The samples obtained were pooled by mixing 100 ng of DNA from each.