RNA-Seq of human CD4 T cells: P5 CD3CD28
After each treatment stimulation we sorted 10,000 live CD4+ T cells in lysis buffer, spun and stored at -80C until processed. RNA isolation was performed as previously described PMID: 35361256. RNA quality was assessed using a Bioanalyzer RNA 6000 Nano Chip (Agilent741 Technologies).