Amplicon of mouse: stool
Stool and colonic tissue samples from mice were collected. Real-time PCR was employed to control cycles and minimize PCR chimera formation. The library was quantified with TapeStation, Invitrogen Qubit 1X dsDNA High- Sensitivity Assay Kits, and sequenced on Illumina NextSeq 2000TM (600 cycles) with 30% PhiX spike-in. DADA2 pipeline was used to infer unique amplicon sequences and remove chimeras.