Whole-chromosome methylation patterns of the mouse inactive X
Whole-chromosome methylation patterns of the mouse inactive X
The embryonic portion of F1 B6Cast placentae at E14.5 was dissected from the maternal portion in phosphate buffered saline before snap-freezing in liquid nitrogen. DNA extraction from E14.5 placentae was performed using the AllPrep DNA/RNA Mini Kit (Qiagen), according to manufacturerâ€™s instructions. Samples were sexed by polymerase chain reaction (PCR) using primers for Otc (X-linked gene) and Zfy (Y-linked gene) as previously described and female samples were selected for further analysis.  Placenta libraries were prepared with the ligation kit SQK-LSK109 (Oxford Nanopore Technologies).