GSM8782660: iPSC-derived motor neurons, DNAJC7 WT homozygote replicate 3; Homo sapiens; RNA-Seq
Cells were harvested in 1mL TRIzol Reagent (Thermo Fisher Scientific) per 1 million cells. 0.2mL of chloroform was mixed with the samples for 3 minutes prior to being centrifuged at 12,000g at 4ºC for 15 minutes. The RNA containing aqueous phase was then transferred to a new tube and 5 μg of Glycogen carrier (Thermo Fisher Scientific) was added to facilitate complete precipitation. Then, 0.5mL of isopropanol was added to the mixtures and left to incubate at room temperature for 25 minutes prior to being centrifuged for 10 minutes at 15,000g at 4ºC RNA was sent to Novogene for QC, and library preparation (250~300 bp insert strand nonspecific library with polyA enrichment)