GSM6886563: RKO_CB103_1; Homo sapiens; RNA-Seq
Cells were collected, RNA was extracted from cell lines using RNeasy Mini Kit (QIAGEN, Cat#74104). RNA quality was assessed on a Fragment Analyzer (Agilent) or TapeStation 4200 (Agilent) and quantified by Qubit 2.0 RNA HS assay (ThermoFisher, Massachusetts, USA) to proceed to the polyA-based stranded mRNA library prep method (scores >7.2). Libraries for mRNA-seq were prepared with the Stranded mRNA Ligation method (Illumina) starting from 1µg RNA, according to manufacturer's instructions. Libraries, all bearing unique dual indices, were subsequently loaded at 250pM in a HiSeq 4000 instrument (Illumina) and sequenced according to manufacturer's instructions, yielding paired-end reads of 75 nucleotides.