WGBS of Mus musculus:adult M Pancreas LCM duct
Genomic DNA (19-50 ng) was fragmented to a target peak of 300-400 bp using the Covaris S2 Focused-ultrasonicator in a 50 l volume according to the manufacturers instructions. The fragmented genomic DNA was subjected to bisulfite conversion using the EZ DNA Methylation-Gold Kit (Zymo Research, cat. #D5005) following the manufacturers instructions. xGen Methyl-Seq DNA Library Prep Kit (IDT, cat. #10009860) was used for WGBS library preparation according to the manufacturers instructions, and the resulting libraries were amplified for 7 PCR cycles using unique dual indexing primers (Swift Bioscience, cat. #X9096-PLATE).