GSM8077759: BMDMs, LA 2; Mus musculus; RNA-Seq
RNA was extracted with RNeasy Kit (Qiagen) with Rnase set (Qiagen). Quality and quantity of RNA was assessed using a NanoDrop™ One (Thermo Fisher Scientific) and Agilent 2100 Bioanalyzer. Library preparation, sequencing at 100 bp read length, and bioinformatics analysis including  genome mapping, gene expression calculation, differentially expressed gene (DEG) detection and principal component analysis (PCA), were performed by BGI (Cambridge, MA)