GSM7747439: WK3_BI15; Homo sapiens; RNA-Seq
scRNA-seq was utilized to study the genome-wide transcriptome profiling of Allo/15BCAR-NKT cells during their in vitro differentiation from gene-engineered HSCs. CB CD34+ HSCs of the same donor were engineered with either Lenti/iNKT-BCAR or Lenti/iNKT-BCAR-IL15 virus, followed by differentiating into AlloBCAR-NKT or Allo15BCAR-NKT cell product, respectively, in the Ex Vivo HSC-Derived CAR-NKT Cell Culture. At day 7, 14, 21, 28 and 42, cell cultures were collected and immediately delivered to the UCLA TCGB Core for library construction and scRNA-seq. Cells were quantified using a Cell Countess II automated cell counter (Invitrogen/Thermo Fisher Scientific). A total of 10,000 cells from each experimental group were loaded on the Chromium platform (10X Genomics), and libraries were constructed using the Chromium Next GEM Single Cell 3' Kit v3.1 and the Chromium Next GEM Chip G Single Cell Kit (10X Genomics), according to the manufacturer's instructions. Library quality was assessed using the D1000 ScreenTape on a 4200 TapeStation System (Agilent Technologies). Libraries were sequenced on an Illumina NovaSeq using the NovaSeq S4 Reagent Kit (100 cycles; Illumina). Library quality was assessed using the D1000 ScreenTape on a 4200 TapeStation System (Agilent Technologies). Libraries were sequenced on an Illumina NovaSeq using the NovaSeq S4 Reagent Kit (100 cycles; Illumina).