GSM8444018: Total RNA from PBMC of FM4 patient pre MT treatment; Homo sapiens; RNA-Seq
Total RNA was extracted using RNeasy Mini Kit (Qiagen, MD, USA) according to the manufacturer's instructions. RNA quality was assessed using Agilent TapeStation 2100 (Agilent). All RNA samples had an RNA Integrity number (RIN) above 7. Libraries generated with NEBNext® UltraTM Directional RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations, after removing rRNA using the Epicentre Ribo-zeroTM rRNA Removal Kit (Epicentre, USA) kit.