GSM8532462: Forelimb, E11.5, H3K4me3, biol rep 1, ChIP; Mus musculus; ChIP-Seq
Nuclei homogenization was performed mechanically and chromatin crosslinking at 1% formaldehyde for 5 min at RT. Chromatin was sonicated (300-500 bp DNA smear) using a Covaris M220 sonicator. ChIP-seq libraries were generated using NEBNext High-Fidelity 2x PCR Master Mix (NEB). The optimum number for enrichment cycles for each library was determined empirically by qPCR, using the same kit, and 1 μL of each ChIP per 10 μL reaction. DNA concentration was quantified by Qbit (Invitrogen).