Target Capture Nuclear DNA of Conradina 'Santa Rosa'
Total genomic DNA was extracted from silica-dried and herbarium specimen leaf material following a CTAB extraction protocol (Doyle and Doyle, 1987). DNA was quantified by Qubit DNA BR assay (Thermo-Fisher Scientific; Waltham, MA, USA). Using the current understanding of relationships in subtribe Menthinae of Lamiaceae, we selected 24 ingroups and 1 representative outgroup species (Monarda punctata, based on Drew et al., 2017)  to be sequenced to address our questions, with our complete dataset made up of 78 samples, and our reduced/one-sample-per-species dataset made up of 25 of those 78 samples. Double-barcoded Illumina libraries were built with a size selection step aiming for >200bp. Target capture reactions were performed with a custom MYbaits kit following the v2.3.1 manual, with libraries pooled 8-plex. The post-capture libraries were pooled and sequenced on six lanes of an Illumina HiSeq3000 with 150-bp paired-end reads. All sequencing and library steps were done by RAPiD Genomics (Gainesville, FL, USA).