GSM8561034: H3K9me3 pAB, WT, Rep2; Homo sapiens; OTHER
Cut&Tag was generated using CUT&Tag-IT Assay Kit (Active Motif, Carlsbad, CA, USA) with modifications recommended in the manufacturer's protocol using Active Motif's pAG-Tn5. 100,000-200,000 HCT116 cells were harvested at room temperature per replicate. Three replicates were prepared for each condition. CUT&Tag-IT Assay Kit (Active Motif, Carlsbad, CA, USA)