DNA sequencing data of semapimod-resistant E. coli
For mutation analysis, 1 mL of overnight culture was pelleted and sent to the Microbial Genome Sequencing Center (MiGS) for DNA sequencing. Libraries were prepared with the Illumina DNA Prep Kit and IDT 10bp UDI indices, and sequencing was performed on an Illumina NextSeq 2000 with 2x151 bp reads. Bcl-convert (v3.9.3) was used for demultiplexing, quality control, and adapter trimming. Reads were aligned to the E. coli BW25113 genome (NCBI CP009273.1) using BWA (v0.7.17)120. Variants were identified using Pilon (v1.23) with default settings121. The only mutation identified was a single base pair substitution in pmrB of a G to an A at position 4322809 of the E. coli BW25113 genome, resulting in a mutation of P94Q.