GSM8706182: HCT116 TIM-mAID DMSO, 40 h set2; Homo sapiens; RNA-Seq
RNA isolation was carried out using the miRNeasy Micro Kit (Qiagen) as per manufacturer's protocol. An additional DNase digest step was carried out using RNase-Free DNase Set (Qiagen) as per manufacturer's protocol. mRNA was isolated using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) as per manufacturer's protocol. RNA libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs) as per manufacturer's protocol.