UCE sequence data of adult Xeromelissa_rozeni
DNA for this sample was extracted from whole specimen tissue using a QiaGen DNeasy Blood and Tissue Kits (Qiagen Inc.). We soaked the tissue in Proteinase-K extraction buffer and incubated overnight at 56C (~12 h). After DNA extraction, samples were processed as dual-indexed Illumina libraries using TruSeq adapters (Faircloth and Glenn 2012). Enrichment was carried out using the Hym-v2 bait set (principal version), We followed the enrichment procedure from Faircloth et al. (2015), with the modifications from Blaimer et al. (2016b, 2016a). Following enrichment, we measured DNA quantity with a Qubit fluorometer (model 3.0, Thermo Fisher Scientific, Inc.) and measured the fragment distribution using a TapeStation (Agilent 2200 TapeStation) and a D1000 HS screen tape (Agilent Technologies, Inc.). We combined pools at equimolar concentrations and size selected for a range of 250-600 bp with a Blue Pippin device (Sage Science, Inc.). Sequencing was carried out on an Illumina MiSeq platform at the Smithsonian Laboratory for Analytical Biology (LAB). The barcode combination for this sample is:  i5: TGAGCTGT and i7: GATGTCGA.