GSM7889104: Control hepatocyte of F95 rep1; Homo sapiens; RNA-Seq
For sequencing, RNA was isolated using RNeasy Plus Kit (QIAGEN) as described in For sequencing, RNA was isolated using RNeasy Plus Kit (QIAGEN) as described in  the product handbook (www.qiagen.com/HB-0405) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for  quantification and bioanalyzer for size distribution detection.