GSM6890229: PaKiT03 NoV∆B2 infected 48hpi; Pteropus alecto; ncRNA-Seq
Cells were washed with PBS twice, TRIzol was then added to the cells, and total RNAs were extracted following the manufacturer's instructions. Extracted total RNAs were used for the construction of small RNA libraries by the method that depends on the 5' monophosphate of small RNAs as described previously with the TruSeq Small RNA Sample Preparation Kit of Illumina (San Diego, CA).