GSM7835933: SFB_03102022_BCR; Mus musculus; OTHER
A single cell suspension was prepared from pooled sorted cells and loaded onto one channel on the 10x Chromium Controller according to the manufacturer's protocol, with a target capture of 10,000 cells per channel. Sequencing libraries were generated using the NextGEM Single Cell 5' Kit v2. Gene expression, BCR, and feature barcoding libraries were pooled and treated with Illumina Free Adapter Blocking Reagent. Sequencing of pooled libraries was carried out on a NextSeq 2000 sequencer, using a NextSeq P2 flowcell, with target depths of 40000, 5000, and 5000 raw reads per cell for gene expression, BCR, and feature barcode libraries, respectively.