GSM8219142: PBS H3K79me2; Mus musculus; ChIP-Seq
BMDCs underwent crosslinking with 1% formaldehyde for 10 minutes at room temperature. The crosslinking reaction was then quenched by adding 125 mM glycine. After reverse crosslinking, the ChIP and input DNA fragments were processed for end repair and A-tailing using the NEBNext End Repair/dA-Tailing Module (E7442, NEB). Subsequently, adaptor ligation was performed using the NEBNext Ultra Ligation Module (E7445, NEB). The DNA libraries were amplified through 15 cycles of PCR and sequenced using Illumina Hi-Seq with paired-end 2×150 sequencing mode.