GSM8782633: A498_SMG1inh_Rep3; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNeasy Plus Mini Kit (Qiagen) according to the manufacturer's instructions, with an additional in-column DNase I treatment (RNase-Free DNase Set, Qiagen). Total RNA was sent to Novogene (Sacramento, CA) for polyA selection and library preparation followed by Illumina sequencing (paired end, 150 cycles). PolyA selection. Performed by Novogene.