GSM7465921: anti-H3K9ac, APOE3-KI; Mus musculus; ChIP-Seq
The sonication program was 20 cycles (30 seconds “ON”, 30 seconds “OFF”) each at High power setting. Two ul of anti-H3K9ac antibody (Millipore, 07-352) was added per IP reaction. The DNA libraries were prepared using NEBNext® UltraTM II DNA Library Prep Kit for Illumina® (NEB, E7103S) following the manufacturer's instruction. The DNA libraries were analyzed using Qubit 4 Fluorometer (Invitrogen, Q33238) and 2100 Bioanalyzer DNA system (Agilent). The pooled libraries were sent to Genewiz, lnc and sequenced 2x150 bp in Illumina HiSeq platform.