GSM8342814: RNAseq_ZT20_Q5A_Cohort1_Rep4; Mus musculus; RNA-Seq
Tissue was resuspended in 800 uL of Trizol and homogenized using a small dounce homogenizer (30 strokes loose, 30 strokes tight) at room temperature. Chloroform was added and aqueos phase isolated. 70 % ethanol was added 1:1, and then passed over an RNAeasy mini-elute column. The Qiagen RNAeasy MicroKit protocol was followed, including all optional steps/DNAse treatment. RNA was eluted in 15 uL and quantitated using a nanodrop spectrophotometer. RNA quality was assessed using a tapestation RNA screentape. 100 ug of total RNA was used as input for library prep. Illunia Stranded mRNA Prep, Ligation