GSM8619477: s8_DSS_IP2; Mus musculus; OTHER
BM cells were isolated as described above. Lineage negative (Lin-) cells were enriched by staining with a panel of biotinylated antibodies against lineage committed cells (CD3, CD45R, CD11b (1:100) Ter-119, Gr-1 (1:20), all from BD Pharmigen). After washing, cells were incubated with streptavidin magnetic beads (Miltenyi) and passed through LS columns (Miltenyi), the negative fraction was collected for further staining with Sca-1 (APC), c-kit (CD117; PE), CD48 (APCCy7), CD150 (BV650), Streptavidin (BV421) (1:200) antibodies and with the viability marker Hoechst 33258 prior to sorting by FACS Aria II. GMPs were identified as Lin-c-kit+ sca-11- CD48+ CD150- cells Cut&Tag libraries were purified using a PCR purification MinElute kit Libraries size and quality were assessed using a 2100 Bioanalyzer instrument (Agilent).