melan-a_ SIRNA DRP1, R3
The libraries were generated with the QuantSeq 3' mRNA-seq Library Prep kit (Lexogen) and oligodT priming. After the first strand synthesis, the RNA was removed. A second strand synthesis was initiated using random priming followed by magnetic bead-based purification. The libraries were then amplified, introducing the sequences required for cluster generation. External barcodes were introduced during the PCR amplification step. Library quantification and quality control were performed using a fluorometer (Qubit., Thermo Fisher scientific) and Bioanalyzer 2100 (Agilent). NGS reads were generated towards the poly(A) tail. Sequencing was performed on NextSeq500 Illumina instrument to produce at least 5 x 106 reads (75 bp SE) for each sample.