GSM7925455: TE-2547-983a-D4-C; Homo sapiens; RNA-Seq
Before extraction cells were rinsed with PBS. TriZol reagent was used to extract the RNA phase, which was subsequently purified with RNA binding columns using the 'PureLink RNA Mini Kit'. DNA depletion of the samples was performed using the 'DNA-free DNA Removal Kit'. Sequencing libraries were prepared from 600 ng total RNA using TruSeq  stranded total RNA library preparation kit with RiboZero Gold treatment (cat# 20020599, Illumina Inc.). Unique dual indexes (cat# 20022371, Illumina Inc.) were used. The  library preparation was performed according to the manufacturers' protocol (# 1000000040499).