RNA-Seq of Homo sapiens
The study aimed to investigate the role of the PACAP/PAC1 signaling pathway in the pathogenesis of Interstitial Cystitis/Bladder Pain Syndrome (IC/BPS) using RNA-Seq transcriptomic analysis. Bladder tissue samples were collected from both IC/BPS patients and control subjects during cystoscopy procedures at Peking University People's Hospital. Total RNA was extracted from these tissues, followed by mRNA enrichment using Oligo(dT) beads. RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit and sequenced on an Illumina NovaSeq 6000 platform to generate paired-end reads. This RNA-Seq approach was designed to identify differentially expressed genes (DEGs) and key signaling pathways associated with inflammation and bladder dysfunction. Bioinformatics analyses, including PLS-DA, GO, KEGG, and GSEA, were performed to explore molecular mechanisms and immune cell infiltration patterns that contribute to IC/BPS pathophysiology. The data generated provide insights into potential therapeutic targets, focusing on the PACAP/PAC2 pathway.