GSM7890533: model-1(MCAO); Mus musculus; RNA-Seq
The samples were rinsed 3 times with Hanks' Balanced Salt Solution (HBSS, Gibco, Cat. No. 14025-076) and minced into 1-2 mm pieces. Then, the brain pieces were dissociated in 2 ml of GEXSCOPE Tissue Dissociation Solution (Singleron) at 37°C for 15 min under stirring conditions. The cell suspension was transferred to 40-micron sterile strainers (Falcon, Cat. No. 352340) and centrifuged (Eppendorf, 5810R) at 300 × g for 5 min to filter the cells. After the supernatant was aspirated completely, the pellets were resuspended in 1 mL of PBS (HyClone, Cat. No. SA30256.01). To destroy red blood cells, 2 mL of RBC lysis buﬀer (Roche, Cat. No. 11 814 389 001) was added to the suspension following the protocol supplied by the manufacturer. The cell suspension was transferred into a microfuge tube, centrifuged again at 500 × g for 5 min at 15-25°C, and then resuspended in PBS. Single cell RNA-seq libraries were constructed  using the GEXSCOPE Single-Cell RNA Library Kit (Singleron Biotechnologies) according to the manufacturer's instructions.