Bulk-RNAseq data of podocytes treated with FSGS sera
Bulk-RNAseq data of podocytes treated with FSGS sera
Cell lysates were collected by buffer RLT from Qiagen RNEasy kit after 24 hours of serum treatment Cells were treated with 10% serum from a patient with focal segmental glomerulosclerosis when they were presenting with FSGS (AP), on vincristine treatment (OT), or in remission (R) for 24 hours.  Qiagen RNEasy kit to extract RNA from cells. Cells are lysed, scraped off vessel, then exposed to spin columns and washes to extract RNA. A gDNAse step was also included.  KAPA mRNA HyperPrep kit to prepare the H. sapiens library for sequencing