GSM8078303: HaCaT, DMEM, 24h, rep1; Homo sapiens; RNA-Seq
Total RNA was isolated from HaCaT cells using Direct-Zol™ RNA microprep kit (Zymo research, CA, USA) according to the manufacturer's protocol. 1000 ng of total RNA was processed to mRNA enrichment using NEBNext Poly(A) mRNA magnetic isolation module (New England BioLabs, MA, USA) and followed by fragmentation, cDNA synthesis, and library preparation with unique dual index using NEBNext Ultra II RNA library prep kit (New England BioLabs) according to the manufacturer's manual.