GSM8405059: troglitazone_0.001_24h_2pool1_S190; Homo sapiens; OTHER
At the 24h and 10d time points media were removed, and spheroids were washed with Dulbecco's phosphate-buffered saline (DPBS; Thermo Fisher Scientific Waltham, MA). Spheroids were lysed by adding 10 µl of 1X TempO-Seq lysis buffer (BioSpyder Technologies Inc Carlsbad, CA) to each well. To ensure complete lysis, spheroids were incubated for approximately 10 minutes at 37°C, pipetted up and down several times, and then frozen at -80 °