GSM8078978: Littermate TNF negative control mice, lung_scRNA; Mus musculus; RNA-Seq
Single-cell suspensions were obtained from the lungs and synovium of TNF-Tg and WT mice (n=3-5 per genotype in lung, 5-10 per genotype in synovium) by a combination of mechanical dissociation and enzymatic digestion.  Tissue was pooled by group and dissociated with GentleMACS Octo Dissociator (Miltenyi Biotec) followed by enzymatic digestion with Collagenase A (COLLA-RO, Roche), Elastase (Worthington, ESL LS002292) and Dispase II (Corning 354235) for lung and type II collagenase (Worthington, CLS-2 LS004174) for synovium. 100,000-300,000 cells were collected and submitted for single cell capture. Single-cell RNA-seq libraries were prepared using Chromium Single-Cell 3′ Library & Gel Bead Kit (version 1.1; 10x Genomics).