GSM8532192: T98G cells,vector-1; Homo sapiens; ChIP-Seq
Briefly, cells were cultured in 10 cm dishes and fixed with formaldehyde to a final concentration of 1%, then incubated at 37°C for 10 minutes. Glycine solution (1.1 ml, 10X) was added and incubated at room temperature for 5 minutes to quench the formaldehyde. The culture dishes were then placed on ice, and the medium was aspirated.Cells were washed twice with 5-10 ml of ice-cold PBS containing 1 mM PMSF. After washing, the cells were scraped into 1 ml of ice-cold PBS with 1 mM PMSF and collected into centrifuge tubes. The pellets were then processed for library preparation using the VAHTSTM Universal DNA Library Prep Kit for Illumina V3 (Vazyme, China), following the manufacturer's instructions, and sent to Lianchuan Bio for sequencing.