CTX_SD_RRBS_2
C57Bl/6J mice were maintained under a 12h/12h light/dark cycle and sleep deprived for 6h from lights on. Animals were sacrificed and brains isolated. DNA was extracted from 1mm punches of brain tissue and 100ng was used to prepare libraries using the Premium RRBS kit V2x96 for low DNA amounts (https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24). Sequencing was performed as 150-bp paired-end sequencing on Illumina platforms by Novogene (Cambridge, UK).