GSM6887015: BMDC, Vehicle_Ctrl, 2; Rattus norvegicus; RNA-Seq
Total RNA was isolated with the RNeasy mini kit (Qiagen) according to the manufacturer's instructions. DNA was digested with the TURBO DNA-freeTM kit (Invitrogen). 1 ug of total RNA was used for the construction of sequencing libraries. Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using PE Cluster Kit cBot-HS (Illumina) according to the manufacturer's instructions.