RNAseq of scalp biopsies from EGFRi treated patients, who exhibited a folliculitis
RNAseq of scalp biopsies from EGFRi treated patients, who exhibited a folliculitis
Parietal scalp (rather than truncal skin) biopsies were taken from long-term (3-9 months) EGFRi-treated patients exhibiting folliculitis (Chronic-EGFRi) or from patients prior to commencing and after two weeks of EGFRi therapy (Acute-EGFRi). Total RNA was extracted using the RNAeasy mini kit (Qiagen, Manchester, UK) according to manufacturer's protocol.  Total RNA was submitted to the University of Manchester Genomic Technologies Core Facility (GTCF).  Illumina TruSeq Stranded mRNA kit, according to the manufacturer's protocol