GSM7746988: PEAT_90FR; Arabidopsis thaliana; OTHER
Total RNA was extracted using either the Spectrum Plant Total RNA Kit (Sigma) PEAT library preparation was done exactly as described in Ni et al., Paired-end analysis of transcriptional start sites, Protocol Exchange  (2010, doi:10.1038/nprot.2010.55), but all steps beginning with the circularization of the PCR product were omitted and the fragments were inserted into NEBNext Ultra™ II DNA Library Prep Kit for Illumina and quantified with NEBNext Library Quant Kit for Illumina. The finished libraries had a fragment size of 500 bp.