GSM8443540: GATA1_KO_D2; Homo sapiens; OTHER
Cells were permeabilized and subjected to nuclear run on with biotinylated NTPs. RNA was isolated, fragmented and nascent RNA enriched for library construction. All steps were performed as described in Mimoso CA, Goldman SR. Current Protocols 2023;3:e961. PRO-seq libraries were constructed as described in Mimoso CA, Goldman SR. Current Protocols 2023;3:e961.