Amplification of V3-V4 region in 16S rDNA of mouse intestinal metagenome: feces
Firstly, the DNA of intestinal flora was extracted from mouse feces. Secondly, universal primers 338F(ACTCCTACGGGAGGCAGCAG) and 806R(GGACTACHVGGGTWTCTAAT) were selected to amplify the V3-V4 region of 16S rDNA. Finally, the Illumina MiSeq platform library was constructed and sequenced to obtain the representative OTU sequence and the subsequent experimental analysis was carried out.