GSM7889190: A549, AFos Knock-In, minus Dox, RNA-seq, rep2; Homo sapiens; RNA-Seq
Cells were lysed by Trizol, extracted by 0.2 volume of chloroform, followed by adding equal volume of 100% ethanol. RNeasy kit was then used to purify the RNA. 3 µg of purified RNA was treated with 2 U of RNase-free DNase I, and purified again with RNeasy kit. Sequencing library was constructed by the Illumina Stranded mRNA Prep kit. 30 million paired-end 50bp reads were obtained for each RNA-seq sample using a NextSeq 2000 instrument.