GSM8128818: KO.TR.21; Mus musculus; RNA-Seq
Cerebral cortices were quickly dissected from the brains of untreated and treated WT and Mecp2 KO animals (P60) and frozen on dry ice. Total RNA was extracted using Purezol (Bio-Rad). RNA integrity was assessed using the RNA 6000 Nano Kit on a Bioanalyzer (Agilent Technologies). RNA samples were quantified using the Qubit RNA BR Assay Kit (Thermo Fisher Scientific). All 24 samples (6 for each experimental group) met the requirement for RNA-sequencing (RIN >7). Illumina RNA-seq libraries were generated using the TruSeq stranded mRNA ligation kit (Illumina) from 1000 ng of total RNA, according to manufacturer's instruction.