GSM8619572: BGLT3 DRIP rep2; Homo sapiens; OTHER
The harvested viral particles were added into the K562 cells plus with 8 µg/ml polybrene for 12 hours. Then the cells were replaced with fresh complete medium and subjected to the following experiments. About 2×107 K562 cells were crosslinked by 1% formaldehyde followed by sonication. Lysates were clarified from sonicated chromatin and RNA-DNA complexes were isolated by S9.6 antibody on Protein A Dynabeads according to the protocol published by Sanz, L.A., et al., 2019. Then the DNA was purified for subsquential processes. Libraries were prepared according to NEB's instructions accompanying the NEBNext Ultra II DNA Library Prep Kit (E7103S) and NEBNext Multiplex Oligos for Illumina (E7335S).