Zea mays B73, primary root tips (0-1mm), DNA copy number, G1 phase, biorep3
DNA-seq libraries were made using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645S) in combination with NEB Unique Dual Index UMI Adaptors (NEB #E7395S). The libraries were constructed using 100 ng DNA input; the adapter dilution was 1:10 and the size selection protocol for obtaining 350-450 bp sequenceable inserts was followed. The libraries were amplified using 7 PCR cycles. The final average library size ranged from 503-540 bp (353-390 bp sequenceable insert + 150 bp adapters). The libraries were sequenced using the Illumina Novaseq 6000, 150 bp paired end.