GSM7925571: Leaf blades, Sufficient boron, 25d,  rep2; Citrus sinensis; RNA-Seq
0.5g of each sample from the treatment and control were sent to Suzhou Panomic Biomedical Technology Co. for transcriptome sequencing. Filtered Reads were compared to the reference genome using the upgraded HISAT2 (http://ccb.jhu.edu/software/hisat2/index.shtml ) software for TopHat2. HISAT2 uses an improved BWT algorithm for library construction. Default parameters are used for non-strand-specific libraries, while strand-specific libraries require the library type to be specified (i.e., use --rna-strandness RF for first, --rna-strandness FR for second).