RNAseq of sciatic nerve from WT versus Adar1cKO mice
RNAseq of sciatic nerve from WT versus Adar1cKO mice
Sciatic nerves from P4 animals of different genotypes were dissected in PBS Total RNA from was extracted using RNeasy Mini kit (Qiagen) according to the manufacturers instructions. After DNAse treatment, concentration and quality of RNA was determined using the NanoDrop (Thermofisher) and Xpose (Trinean) apparatus. RNAseq libraries were prepared starting from 60 ng of total RNA using the Universal Plus mRNA-Seq (Nugen) as recommended by the manufacturer. The oriented cDNA produced from the poly-A+ fraction were PCR amplified (15 cycles).