GSM8264977: Heart_LPS_rep3; Mus musculus; RNA-Seq
After 12 hours of treatment, heart tissues were collected from the mice. Total RNA was extracted from myocardial tissue samples using TRIzol reagent and purified with chloroform, isopropyl alcohol, and ethanol. The quality of RNA samples was evaluated by concentration, 28S/18S ratio, and RNA quality number (RQN) measured by a fragment analyzer with the Standard Sensitivity RNA Analysis Kit (15 nt) (DNF-471). mRNAs were enriched from high-quality RNA samples (RQN＞7.0, 28S/18S＞1.0), fragmented and used as templates for synthesizing cDNAs. cDNAs were subjected to end-repair, add a single 'A' nucleotide to the 3' ends, set up to ligate adaptors with the cDNAs, and finally be amplified to construct libraries. After quality control, cDNAs were circularized to generate DNA nanoballs via rolling cycle amplification, which were then loaded into patterned nanoarrays using high-intensity DNA nanochip technique and sequenced through combinatorial Probe-Anchor Synthesis.