Amplicon of bioreactor assembly: bioreactor liquid outflow
DNA was extracted from outflow liquid of bioreactors, using a DNeasy UltraClean Microbial Kit (QIAGEN). The V3/V4 region of the 16S rRNA gene of extracted DNA was amplified using primers 338F (5-ACTCCTACGGGAGGCAGCAG-3) and 806R (5-GGACTACHVGGGTWTCTAAT-3) on the Illumina platform by a NovaSeq 6000.