RNA-Seq of Hedophyllum nigripes: experiment 2 mesocosm 2
Total RNA extraction was conducted using a CTAB extraction followed with a commercial Qiagen kit. The quantity and purity of the extracted RNA were evaluated using a Nanodrop ND-1000 Spectrophotometer (ThermoFisher), which measures RNA concentration at 260 nm and assesses purity by detecting the presence of other compounds such as DNA at 230 nm and proteins at 280 nm. The integrity of total RNA was determined by automated capillary electrophoresis using an Agilent 2100 Bioanalyzer (Agilent Technologies). The cDNA libraries were constructed by poly(A) enrichment and sequenced on a NovaSeq 6000 instrument by the Genome Quebec platform. The 100bp paired reads were clipped using default values of the Illumina software.