GSM8342491: P14 Tpex Ctrl #3; Mus musculus; RNA-Seq
Cells were sorted in lysis reagent (QIAGEN). After addition of chloroform (Sigma) and centrifugation, RNA was isolated from the aqueous phase using an RNEasy Micro kit (QIAGEN). 1 ng RNA from 10,000 sorted cells was used as input for the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB).