RNAseq of Gadus morhua: feces
Gut microbiome content (20 mg) was extracted for total RNA using E.Z.N.A. Total RNA Kit II (Omega Bio-tek), with between 3 and 5 samples at each time point in control and oiled juvenile Atlantic cod. RNA concentrations were measured with a Qubit 4 fluorometer (Invitrogen), and RNA integrity assessed with an Agilent 2100 Bioanalyzer. Samples used for downstream library preparation had RNA Integrity Numbers > 8. Paired end, 2 x 75 bp sequencing was performed by the University of California, Riverside Genomics Core.