GSM8265689: IT4 SLI_BioID-Pos2; Plasmodium falciparum; RNA-Seq
Synchronous ring-stage parasites with a parasitemia of 3 - 5%, dissolved in 5 pellet volumes of Trizol (Thermo Fischer). RNA isolation with Qiagen miRNeasy Mini Kit according to the manufacturer's instructions. RNA integrity was assessed using the Agilent 2100® bioanalyzer system with the RNA 6000 Pico Kit. All samples had a RIN > 8. Ribosomal RNA was removed using QIAseq FastSelect RNA Removal Kit. Libraries were prepared with the QIASeq Stranded mRNA Library Kit and sequenced on an Illumina NextSeq 550 system with NextSeq 500/550 Mid Output Kit v2.5 (150 cycles).