GSM8782280: ctrl-RXR-2; Mus musculus; ChIP-Seq
Brain lysates of mice treated with Bexarotene or Vehicle were sonicated with 3 pulses of 15 sec at amplitude 30, a 120 sec pause and 3 pulses of 15 sec at amplitude 40 using a Model 705 Sonic Dismembrator (Fisher Scientific, Pittsburgh, PA), to obtain fragments of 200–600 bp. ChIP libraries were generated using TruSeq ChIP Sample Prep Kit (Illumina, San Diego, CA) following manufacturer's protocols. For each of the steps, samples were purified by AMPure XP beads (Beckman Coulter, Brea, CA). Adapter-ligated samples were separated on a 2% agarose gel to obtain 250–300 bp size-range of DNA fragment to remove unligated adapters. The libraries were validated by Agilent Technologies 2100 Bioanalyzer to check the size, purity, and concentration of the sample before the sequencing