GSM8561507: naive B cells #1; Homo sapiens; RNA-Seq
RNA isolation was performed using the RNeasy Micro Kit (Qiagen) Barcoded mRNA-seq cDNA libraries were prepared from 100 ng of total RNA using NEBNext® Poly(A) mRNA Magnetic Isolation Module and NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® according to the manual with a final amplification of 12 PCR cycles. Quantity was assessed using Invitrogen's Qubit HS assay kit and library size was determined using Agilent's 2100 Bioanalyzer HS DNA assay. RNA Seqeuncing (PE150)