DNBSEQ-G400 paired end sequencing of SAMD00575149
Fragmentation and amplification of ATAC-seq libraries were conducted according to Corces et al.. Briefly, approximately 50,000 cells were lysed and transposition reaction was performed with the Tn5 Transposase (Illumina, FC121-1030) at 37?C for 30 minutes. The reaction liquid was purified with Qiagen MinElute PCR Purification Kit. Then 5 cycles of PCR with custom Nextera PCR primers (from Wu and Buenrostro 2013) were conducted using NEBNext Q5 Hot Start HiFi PCR Master Mix (New England Biolabs; NEB). Additional number of PCR cycles was determined by qPCR of the partly amplified products, following Buenrostro et al.29. The PCR products were purified with Agencourt AMPure XP beads (Beckman Coulter, A63881), using double size selection (0.65x /1.8x) according to the manufacturer's protocol.