GSM8404658: Ddit3; Mus musculus; OTHER
1 mg of fresh tissue was weighed for each antibody/MNase reaction, with an additional 1 mg for the input sample. Reactions included positive and negative controls. Tissues were fixed with 1 ml of 0.1% formaldehyde for 10 minutes and quenched with glycine for 5 minutes. Cut&Run was conducted on mouse liver tissue samples from mice injected with APAP for 6 hours using the Cut&Run assay kit (CST, 86652). For Cut&Run, purified DNA was used to prepare sequencing libraries with the NEB Next Ultra II DNA Library Prep Kit for Illumina (7645S and 7335L)