MethylSeq of Lygodium microphyllum pooled gametophytes
DNA was extracted from samples following a modified CTAB extraction and purification with the Qiagen Plant DNeasy kit. Methyl-seq libraries were constructed using the NEBNext Enzymatic Methyl-seq Kit and sequenced to an average depth of 10x  per library on the Illumina NovaSeq Sp4 at the University of Florida ICBR.