GSM8560924: Cerebellum_D801N_442; Mus musculus; RNA-Seq
Animals were euthanized by CO2 asphyxiation and tissues were harvested, frozen on dry-ice, and stored in -80°C freezer until use. The RNA was isolated using RNeasy Mini Kit and DNAse-treated following the manufacturer's protocol (Qiagen). Libraries were prepared from DNase-treated total RNA using the KAPA mRNA HyperPrep Kit. Briefly, the protocol includes isolation of polyA containing mRNA using oligo-dT magnetic beads, RNA fragmentation, first and second strand cDNA synthesis, ligation of Illumina-specific adapters containing a unique barcode sequence for each library, and PCR amplification.