Total RNA-Seq MHV68 Infection Models
A20-HE-RIT cells were induced in 1x RPMI media containing 5 ug/mL Doxycycline and 20 ng/mL 12-O-Tetradecanoyl-phorbol-13-acetate (TPA) for 6 hours. Total RNA was isolated from cells using the Direct-zol RNA MiniPrep Kit. ERCC spike-in controls were added. RNA was ribominus selected and directional cDNA libraries were generated using either Stranded Total RNA Prep with Ribo-Zero Plus (Illumina # 20040525) or TruSeq Stranded Total RNA Ribo-Zero Gold (Illumina #RS-122-2303). Sequencing was performed using the Illumina NovaSeq SP platform to generate 150 bp PE reads.