GSYA_NGS
Fragmentation, adapter ligation, and PCR enrichment were used to prepare pre-libraries from DNA extracted from FFPE samples and fresh tissues. Subsequently, RNA probes with specific sequences were hybridized with the pre-libraries to specifically capture partial exonic and intronic regions from the human genome. The DNA library captured by the probes was then enriched using magnetic bead-based methods, and the captured library was quantified and quality controlled. Finally, the quantified library was subjected to high-throughput sequencing using a genetic sequencer.