GSM7890693: Day7_KD_2; Homo sapiens; RNA-Seq
Cartilage pellets were ground in TRIzol Reagent (200 µl; ThermoFisher Scientific) and stored at -80 degrees until required. RNA was extracted using a standard phenol/chloroform extraction and purified using RNeasy minicolumns according to the manufacturer's instructions (Qiagen, Basel, CH). 3 technical repeats were then pooled per donor to provide sufficient material. DNA was removed using an off-column DNase kit (DNA-Free; ThermoFisher). Sequencing library preparations were performed using the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (New England Biolabs, Hitchin, UK) following the manufacturers protocol.