GSM8532399: MIA PaCa-2 AMG510_Myc; Homo sapiens; ChIP-Seq
The iDeal ChIP-seq kit for Transcription Factors (Diagenode) was used for ChIP-seq.  Genomic DNA was sonicated for 30 seconds on/30 seconds off (easy mode) for 4 cycles using a Biorupter (Diagenode) to achieve an average fragment size of 200bp. 4ug of cMYC antibody (Abcam ab32072) was used for immunoprecipitation. 1-20 ng ChIP DNA from each sample was end repaired and A-tailed, then ligated with NEB adapters, following by 2xKAPA HiFi HotStart mix and NEB dual indexes PCR to enrich fragments between 200-500 bp. Libraries were quantified and quality checked using the Bioanalyzer 2100 (Agilent) and sequenced on an Illumina NovaSeq 6000 (150-nucleotide paired-end reads).