GSM8265293: Zebrafish, scRNAseq, shield-Mrbm24a-1; Danio rerio; RNA-Seq
For the preparation of single-cell suspensions, a total of thirty-three mutant embryos of Mrbm24a or wild-type embryos at the shield stage were collected. These cellular suspensions were then loaded onto a 10X Genomics GemCode Single-cell instrument, which generates single-cell Gel Bead-In-EMulsion (GEMs). Libraries were generated and sequenced from the cDNAs using Chromium Next GEM Single Cell 3' Reagent Kits v3.1. Upon dissolution of the Gel Bead within a GEM, primers containing an Illumina® R1 sequence (Read 1 sequencing primer), a 16 nt 10x Barcode, a 10 nt Unique Molecular Identifier (UMI), and a poly-dT primer sequence were released. These primers were mixed with the cell lysate and Master Mix. Full-length, barcoded cDNAs were then reverse-transcribed from poly-adenylated mRNA