Transcriptomic signatures of progressive and regressive liver fibrosis and portal hypertension
Transcriptomic signatures of progressive and regressive liver fibrosis and portal hypertension
Animal experiments were performed in accordance to the approval by the Austrian Federal Ministry of Education, Science and Research (BMBWF-V/3b/2020-0.398.007). The organs of Mus musculus C57BL/6J were collected after acquiring the study measurements and following euthanasia under anesthesia between mid-May and mid-June 2021, and stored at -80°C prior to RNA extraction.  Fibrosis was induced by the administration of carbon tetrachloride (CCl4) or thioacetamide (TAA). CCl4 was gavaged as a 20% v/v solution in olive oil at a dose of 2 mL/kg body weight three times a week. TAA was injected intraperitoneally in 0.9% saline at a dose of 150 mg/kg thrice a week. The dose was gradually increased over the first week for CCl4 administration and over the first two weeks for TAA administration. The total time of fibrosis induction was 12 weeks, followed by one or two weeks of regression.  Each sample represents total RNA from murine liver, extracted using QIAGEN RNA Easy Mini Kit, and stored in -80°C prior to submission. The library preparation was done with a TruSeq Stranded mRNA LT sample preparation kit (Illumina, CA, USA).