GSM8339567: Sample_Control Telo; Mus musculus; RNA-Seq
Unwounded epidermal samples were prepared as above, and single cells were gated on DAPI for dead cell exclusion, negative for non-epidermal markers (CD140a, CD117, CD31, and CD45; hereafter referred to as “Dump gate”) and SCA1 (LY6A/LY6E), and positively selected on high expression of integrin-a6 and CD34. Library preparation using the Illumina TrueSeq mRNA sample preparation kit was performed at the Weill Cornell Medical College Genomic Core facility (New York), and RNAs were paired-end sequenced on Illumina HiSeq 4000 machines.