GSM8172981: Sbj32_OnT; Homo sapiens; RNA-Seq
Total RNA was extracted by AllPrep DNA/RNA FFPE Kit (Qiagen N.V.) according to the RNA extraction only protocol provided by the manufacturer. Total RNA was eluted with 30 μL RNase-free water. cDNA library for RNA-seq was prepared for the directional sequencing method with the depletion of ribosomal cDNA and the unique-dual indexes using the following kits and reagents: Takara Bio Inc, SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian (Takara Bio Inc.). In case that RNA concentration was ≥25 ng/μL, 25 ng of RNA was used for library preparation. In case that RNA concentration was <1.25 ng/μL, the RNA samples were concentrated by evaporation, and then all volume of RNA samples was used for library preparation. Otherwise, 10 ng of RNA was used for cDNA library preparation.