GSM8477182: osteal macrophage isolated from ovariectomized mice2; Mus musculus; RNA-Seq
Isolated osteal macrophages were lysed with TRIzol Reagent (Invitrogen, Thermo Fisher Scientific, CA) and RNA was extracted using NucleoSpin® RNA (TAKARA, Tokyo, Japan) according to the manufacturer's instructions. Libraries were generated from 1 µg of RNA using a NEBNext Ultra II Directional RNA Library Prep Kit (MA, USA)