miRNA-Seq of SK-N-SH Human Neuroblastoma
miRNA was extracted from Human neuroblastoma SK-N-SH cell using mirVana miRNA Isolation Kit and quantified on nanodrop and Small RNA sequencing (smRNA) libraries were constructed using QIAseq miRNA Library Kit. The Illumina-compatible sequencing libraries were quantified by Qubit fluorometer. The fragment size distribution of the libraries was analyzed on Agilent 2200 TapeStation. Thus, the libraries were sequenced using NovaSeq 6000 with 150*2 chemistry.