GSM8264995: Fgf10/Flrt1/3 TKO replicate3; Mus musculus; RNA-Seq
E15.5 of mutant embryos were removed from the uterus of mutant females, and stored in ice-cold in Leibowitz medium with 5% FBS. Brains were isolated and cut in 300 μm using a vibratome (Leica VT1000S, Germany) in cutting buffer, Leibowitz medium with 5% FBS. Then, brain sections were manually dissected for both hemisphere of cortical primordium of cortex. Meanwhile, genotyping was performed. Then, collected tissues were transferred into a dissociation Buffer, EBSS. Dissociation was performed with Worthington Kit and manually Papain dissociation system was carried out according to the recommended protocol (Worthington, #LK003163). For experiments utilizing the 10x Genomics platform, the following reagents were used Chromium Single Cell Next GEM Single Cell 3' GEM kit v3.1 (PN-1000130), Library Construction Kit (PN-1000196), Chromium Next GEM Single Cell 3′ Gel Bead Kit v3.1 (PN-1000129) and Single Index Kit T Set A (PN-1000213) and Dual Index Kit TT Set A (PN-1000215) were used according to the manufacturer's instructions in the Chromium Single Cell 3′ Reagents Kits v3.1 User Guide.