GSM8561795: 4_EGF_IFNgamma_treated_cells_R1; Homo sapiens; RNA-Seq
RNeasy Mini kit (Qiagen, CA) with DNase digest and QIAcube automation stations. The RNA was quantified, and the quality was assessed by Bioanalyzer analysis (Agilent Technologies, Santa Clara, CA). Clontech Smart-Seq V4 Ultra Low Input RNA kit (Takara Bio, Shiga, Japan) in combination with the Illumina NexteraXT kit (Illumina, San Diego, CA, USA), as per manufacturer's instructions, with 10 ng of total RNA as input.