GSM8560683: YKL_6h_637; Homo sapiens; RNA-Seq
Total RNA was isolated from cells with RNeasy Plus micro kit (Qiagen) The integrity of total RNA samples was evaluated with BioAnalyzer 2100 system (Agilent). Sequencing compatible libraries are prepared with NEBNext Ultra II RNA Kits (NEB) and NEBNext Multiplex Oligos for Illumina – 96 Unique Dual Index Primer Pairs (NEB) following instructions from manufacturer. In brief, total RNA was poly(A)-enriched, fragmented, and reverse transcribed to cDNA. cDNA were ligated with adaptors and indexes. Sequencing compatible libraries were then quality-controlled with BioAnalyzer 2100 system (Agilent) and sequenced