Whole-chromosome methylation patterns of the mouse inactive X
Whole-chromosome methylation patterns of the mouse inactive X
Neural stem cells were derived from E14.5 cortical dissections of embryos resulting from a cross between XistKO/+ 129 dams and Cast sires.  High-molecular weight DNA from 2M neural stem cells was extracted with the Monarch HMW Cell kit (New England Biolab) with agitation at 300 rpm. NSC libraries were prepared with the ultra-long kit (SQK-ULK001).