GSM7116590: liver-Loxp-1; Mus musculus; RNA-Seq
Total RNA was processed using mRNA enrichment or rRNA removal methods. 1.Fragmentation of the obtained RNA using an interrupt buffer, reverse transcription with random N6 primers, and synthesis of cDNA double stranded DNA. 2.The ends of the synthesized double stranded DNA were flattened and phosphorylated at the 5 'end, forming a sticky end protruding from an "A" at the 3' end, and then connected to a bubbly junction with a protruding "T" at the 3 'end. 3.The connecting products were amplified by PCR using specific primers. 4.The PCR product was thermally denatured into a single strand, and then cyclized with a bridge primer to obtain a single stranded circular DNA library. The ends of the synthesized double stranded DNA were flattened and phosphorylated at the 5 'end, forming a sticky end protruding from an "A" at the 3' end, and then connected to a bubbly junction with a protruding "T" at the 3 'end. The connecting products were amplified by PCR using specific primers. The PCR product was thermally denatured into a single strand, and then cyclized with a bridge primer to obtain a single stranded circular DNA library.