GSM8265189: Keto_Veh_1 heavy polysome; Homo sapiens; OTHER
Tumors were isolated from mice with different treatment at same time. Then tumors were lysed and lysates were seperated using sucrose gradient, and fractions 2-7, and 8-13 were pulled together, same amout of ERCC was added to each pool, PureLink™ RNA Mini Kit (ThermoFisher). 10ng of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian following manufacturer's protocols.