GSM8443704: S4_mock; Mus musculus; RNA-Seq
Lungs from mice at day 3 p.i. were processed to single-cell suspensions as described above. Single-cell suspensions were subjected to magnetic sorting using MojoSort™ Mouse anti-PE Nanobeads (BioLegend, #480080) to remove IV-positive, PE-tagged cells. Suspensions were subjected to magnetic separation a total of four times before the negative fraction was resuspended in buffer and counted. Cell suspensions were captured in emulsion droplets using the Chromium Next GEM Single Cell 5' GEM and Gel Bead Kits (10x Genomics, #1000244 and #1000264) on a 10x Chromium Controller. Amplification of cDNA and 5' GEX library preparation and indexing were performed according to the manufacturer's instructions using the Library Construction Kit and Dual Index Kit TT Set A (10x Genomics, #1000190 and #1000215).