GSM8498358: IgG47; Homo sapiens; RNA-Seq
Fresh tissues were stored at -80°C in RNA later RNA Stabilization Reagent (QIAGEN, Hilden, Germany). The tissues were then homogenized using a bead mill at 3,200 rpm for 30 seconds in homogenization solution equipped with Maxwell RSC simply RNA tissue kit (Promega, Madison, WI). The Maxwell RSC simply RNA tissue kit (Promega) was used to extract total RNA, and mRNA was purified using Oligo dT beads (NEBNext Poly (A) mRNA magnet Isolation Module, New England Biolabs, NEB). The NEBNext Ultra II RNA Library Prep Kit (NEB) and NEBNext Multiplex Oligos for Illumina (NEB) were used to generate cDNA libraries for Illumina sequencing. An Agilent 4200 TapeStation. The confirmed libraries were mixed in equal molecular amounts for clustering and sequencing on an Illumina NovaSeq 6000 DNA sequencer with a 50 bp paired-end cycle sequencing kit (Illumina, San Diego, CA).