Oxford Nanopore Sequencing of Lygodium microphyllum
HMW DNA was extracted from pooled sporophyte tissues using a nuclei isolation (Mariac et al., 2022) with woody plant buffer (Loureiro et al. 2007), SDS-based extraction (Schalamun et al. 2019), and high salt clean-up (Fang et al. 1992). Prior to extraction, plants were dark-treated for at least 48 hours to reduce photosynthate and other organic compounds during the extraction process. Size selection was performed with a PacBio SRE XL eliminator kit.