GSM7116979: L2, replicate 2, scRNAseq; Homo sapiens; RNA-Seq
Control, PDGFB- or TGFb1-induced microHOs (n>30 per group) were harvested on day 21, and single cell suspensions were prepared by protease digestion The single cell suspensions were visually inspected under a microscope, cells were counted using a Scepter™ 2.0 Handheld Automated Cell Counter (EMD Millipore, Burlington, MA), and then resuspended in PBS with 0.01% BSA. scRNA-seq libraries were prepared by using the spit-pool based scRNA-seq method (Evercode™ WT Mini v2 from Parse biosciences) according to the manufacturer's instructions. In brief, 5000 cells from each group were split into 3 wells for the first round of sample barcoding, and the final pooled cells were divided into two equal libraries for sequencing.