S4
Mosquito pools were homogenized in a mixer mill MM400 (Retsch GmbH, Germany) for 10 min at 20/s after addition of 1 ml of MEM (Gibco, USA) and three 3-mm steel balls to each tube. After centrifugation at 12,000  g for 30 min, the supernatant was forwarded to viral RNA extraction using MagMAX Pathogen RNA/DNA Kit (Thermo Fisher, Lithuania). The ribosomal RNA was removed using Ribo-off rRNA Depletion Kit (Bacteria) (Vazyme, #N407). Prokaryotic RNA library was prepared using MGIEasy RNA directional library preparation kit V2.0 (16 RXN). The 100 bp paired-end sequencing was performed on DNBSEQ (DNBSEQ Technology) platform.