GSM8078321: BMDMs, AAV-SHISA3-2; Mus musculus; RNA-Seq
Total RNA from cells were extracted with Trizol reagent (Magen) according to the manufacturer's instructions. The quantity and quality of RNA were measured by Nanodrop. 4 ug of total RNA was used for the construction of sequencing libraries. Sequencing libraries were generated using VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina ® （NR604-01/02）following the manufacturer's recommendations and index codes were added to attribute sequences to each sample