CD34+ umbilical cord cells transduced with wild type SRSF2 expressing lentivirus
Umbilical cord CD34+ cells were transduced with lentiviral vectors expressing GFP, and GFP fusions of wild type SRSF2, SRSF2 P95H, or SRSF2 P95R. Seven days post transduction GFP positive cells were isolated by flow sorting. RNA was extracted from the sorted cells using the Total RNA Purification Plus Micro Kit. rRNA depleted RNA-Seq libraries were generated using the Stranded RNA-Seq Kit with RiboErase. The libraries were subjected to 100 nt paired-end sequencing on Illumina Hi-Seq 4000.