GSM8561715: MHCC97H_Input; Homo sapiens; ChIP-Seq
For ChIP-seq, DNA samples were sonicated in a Biorupter Pico (Diagenode) to acquire nearly 300 bp fragments. Next, the MicroPlex Library Preparation Kit (Diagenode) was employed for library preparation, followed by sequencing through the HiSeq 2000 Illumina platform of the NIG. The quality of the ChIP-seq raw data files were evaluated through FASTQ quality check (FASTQC). FastQ files were evaluated in the public server usegalaxy.org where the sequence reads were aligned to the mouse reference genome (mm9) using Bowtie2 (version 0.4) with default parameters. For ChIP analysis, MHCC97H cells were fixed employing 1% formaldehyde for 30 min, followed by adding 1.25 mol/L glycine for 5 minutes to end the reaction.