GSM7890515: CT26, tumor, Glutaminase 2 [T2LP]; Mus musculus; RNA-Seq
Tissue lyzed in RLT buffer (Qiagen) using Tissuelyzer. Total RNA extracted using RNAeasy kit (Qiagen) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.