GSM7746306: Control U266 cell line sample 3; Homo sapiens; RNA-Seq
Total RNA was isolated from U266/Control and U266/MYC cells using RNeasy Mini Kit (Qiagen) according to manufacturer's protocol. A starting amount of 500 ng of RNA was used to prepare poly-A enriched, single barcoded libraries using the NEBNext® Ultra™ RNA Library Prep Kit. Quality control of the libraries was evaluated by Bioanalyzer analysis with High Sensitivity chips. Sequencing was performed on a HiSeq 4000 ,