GSM8078238: Fibroblasts, 2 week Ficoll No TGFB, rep3; Homo sapiens; RNA-Seq
Total RNA was purified using Monarch Total RNA Prep miniprep kits (NEB) before quantitation on a NanoDrop One spectrophotometer Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification