Merlin rescue in meningioma mouse xenografts
3 million CH157-MN meningioma cells hosting doxycycline inducible WT Merlin were injected into the right flank of 4-6wk old NU/NU mice. Doxycycline (20ug/ml) was supplemented in the water of the treatment group at the time of injection. Tumours were dissociated using Collagenase type 7 and 0.25% trypsin. Single cells were processed through a 10X Genomics Chromium Controller and libraries were created using the Chromium Single Cell 3 Library & Gel Bead Kit v3. Libraries were sequenced on a NovaSeq S4 6000.