scNanoATAC-seq2 of Mus musculus: Technical tests on cell lines
Each single cell was picked into a 200-µl PCR tube containing 2 µl ice cold lysis buffer and then subjected to transposition. And then to termination and digestion, incubated at 25°C, 5 min; 55°C, 30 min; 4°C pause. Library was constructed by genomic DNA purification and amplification with cell barcodes. Long-read amplicons with different cell barcodes were pooled together and subjected to PCR amplification and purification. After that, each long-read amplicon fragment library was loaded into one R9 chip and sequenced on the Oxford Nanopore PromethION 48