T490_CM_C3P18_UDID088_ATAC_R07L01S01_CONTROL
Detailed methods are found in doi 2024.05.03.592380. Frozen nuclear pellets were thawed on ice and tagmented in total volume of 25ul in permeabilization buffer containing digitonin (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 3 mM MgCl2, 0.01% digitonin) and 2.5ul of Tn5 from Nextera DNA Library Preparation Kit (Illumina) for 45-75min at 37C in a thermomixer (500 RPM shaking). To eliminate confounding effects due to index hopping, all libraries within a pool were indexed with unique pairs of i7 and i5 barcodes. Libraries were amplified for 12 cycles using High-Fidelity 2X PCR Master Mix (NEB) in total volume of 25ul in the presence of 800 nM of barcoded primers (400nM each) custom synthesized by Integrated DNA Technologies (IDT) and sequenced with 150-bp paired end reads on a HiSeq4000.