DNA-Seq of homo sapien: feces
Using extracted DNA as a template, bacterial 16S rRNA genes were PCR-amplified with barcoded primers (343F/798R) and Takara Ex Taq polymerase, targeting the V3-V4 regions. PCR products were purified with AMPure XP beads and quantified with Qubit before sequencing on the Illumina NovaSeq 6000 platform to generate 250 bp paired-end reads.