WGS of Bradyrhizobium Ash2021
Genomic DNA was extracted using Qiagen Blood and Tissue kits, as per manufacturer's recommendations. Libraries were prepared by tagmentation using the Illumina DNA prep kit where Illumina adaptors and barcodes were added to TruSeq primers S501-S508 and N701-N712 (5). Sequencing was performed on Illumina NextSeq 550 platform with 2 x 150 bp read length and supplementary long read sequencing was performed on the Oxford Nanopore Technology (ONT) platform.