rat 80, tMCAO after saline administration
The rat was injected intraperitoneally three times with saline at 90 min, as well as 2.5 and 6.5 hours after tMCAO. At 24 h after tMCAO, rat was decapitated, the dorsolateral region of frontal cortex was dissected and total RNA was isolated. The mRNA fraction was subjected to High-throughput RNA-sequencing (RNA-Seq). Sequencing was done using an Illumina HiSeq 1500 instrument. At least 10 million reads (1/50 nt) were generated.