16S rRNA on day 0 of hyperthermophilic composting H0.3
The variable V3-V4 region of 16S rDNA was amplified using Phusion High-Fidelity PCR Master Mix (M0532S, New England Biolabs, Beijing) and the specific primers 341F (5-CCTAYGGGRBGCASCAG-3) and 806R (5-GGACTACNNGGGTATCTAAT-3) with the barcode. The constructed library was sequenced on an Illumina NovaSeq 6000 platform (PE250 mode, Novogene, Tianjin, China). The effective tags were generated from the raw sequences using FLASH (version 1.2.1.1) for sequence assembly, and fastp (version 0.23.16) for data filtration and UCHIME Algorithm for chimera identification.