metagenome of soil: Crayfish pond
Microbial cell DNA in C, RC, and FC samples was extracted using E.Z.N.A.@ water and soil DNA (Omega Bio-Tek, lnc., Norcross, GA, USA)kits according to the standard procedures of the manufacturer's instructions, respectively. All DNA samples were preserved with sufficient dry ice and sent to Meiji Biotechnology Co., Ltd. (Shanghai, China) for library construction and Illumina HiSeq sequencing (2  101 bp paired-end sequencing, Hiseq3000 kit V6).