scRNA-Seq of mus musculus: intestinal lamina propria leukocytes
Small intestinal lamina propria leukocytes were isolated from two mice infected with N.brasiliensis for seven days. CD45+CD19-immune cells were further sorted with flow cytometry. Library preparation and sequencing were performed by Singleron Biotechnologies. The scRNA-seq libraries were sequenced on an Illumina novaseq 6000 with 150 bp paired end reads.