RNAseq of proximal colon of female germ-free mouse #2 associated with fecal microbiota from healthy patients
The libraries for sequencing were prepared using Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus kit with 100 ng of RNA as input. The quality of final libraries was tested on TapeStation 4150 (Agilent) and quantified with PicoGreen reagent (ThermoFisher). All libraries were diluted to the 4 nM final concentration and combined into equimolar pooled library prior to sequencing. The pooled library was diluted, denaturated, and sequenced on NextSeq 550 with High Output chemistry