RNA-Seq of Euglena gracilis, rRNA depleted
E. gracilis cells (strain FACHB-848) were obtained from the Freshwater Algae Culture Collection at the Institute of Hydrobiology (Wuhan, China) and cultured in HUT medium according to the manufacturers instructions. Total RNA for RNA-seq was extracted from approximately 1108 E. gracilis cells, and ribosomal RNA was removed using specific probes. A strand-specific library was constructed by synthesizing first strand cDNA with random hexamer primer and M-MuLV transcripase (RNase H-) and second strand cDNA with DNA polymerase I and RNase H. The qualified library was sequenced on a Illumia Nova 6000 platform by Novogene (Beijing, China).