HiFi of Musca autumnallis: unfed adult male face fly
A single individual was prepared to undergo DNA extraction and PacBio HiFi preparation. The sample was homogenized in a 1.5 mL tube in a liquid nitrogen bath with a liquid nitrogen-cooled pestle. The Nanobind Tissue Kit (PacBio, Menlo Park, CA, USA) was used to obtain sufficiently high-molecular weight DNA.