RNA-Seq of biofilm sample collected on day 79 from the top of TBR that was inoculated with anaerobic sludge
DNA extraction of samples was done using a slightly modified version of the standard protocol for FastDNA Spinkit for Soil (MP Biomedicals, USA). Amplicon libraries for the archaea/bacteria/eukaryota 16S/18S rRNA gene variable regions 4-8 (abeV48-A) were prepared using a custom protocol. The forward and reverse primers used include custom 24 nt barcode sequences followed by the sequences targeting abeV48-A: [515FB] GTGYCAGCMGCCGCGGTAA and [1391R] GACGGGCGGTGWGTRCA.