Shp2MKOApoE2
Single aortic cells were prepared as previously described. Single-cell suspensions were filtered with 70-m cell strainers and incubated with MicroBeads conjugated with an F4/80+ antibody (130-110-443, Miltenyi Biotec) in accordance with the protocol approved by Mitenyi Biotec. The single cells binding to the MicroBeads were collected for RNA-seq analysis.