Human gut microbiota from DRE subject, Sample DRE9
Bacterial genomic DNA in stool samples has been extracted as previously described22 by using the Spin stool DNA kit (Stratec Molecular, Berlin, Germany), according to the manufacturers instructions. Extracted DNA quantification was determined using the DNA High Sensitivity Qubit kit (Thermofisher Scientific, Waltham, MA, USA). The sequencing of the V3-V4 hypervariable regions of the bacterial 16S rRNA gene was performed by a service offered by Macrogen (Seoul, Republic of Korea), according to the Illumina 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA).