Mosquito #205, unexposed, collected at 7DPF (at RU) - Carcass
RNA was extracted from a single mosquito carcass with TRIzol and the NucleoSpin RNA kit (Macherey-Nagel). Libraries were rRNA-depleted with the Zymo-Seq RiboFree kit (Zymo Research) and indexed with the Illumina Stranded mRNA kit. Libraries were sequenced on an Illumina instrument and yielded a minimum output of 30mln 2x100bp reads.