Meiosis_screening_library
This is a smaller sublibrary (12500 cells instead of 62500) that was used as a pilot experiment. Cells containing integrated expression vectors were seeded in mTeSR1 containing 10 M Y-27632, 5 M GSK3484862, and 1 g/mL doxycycline. After 24 hours, the medium was changed to APEL2 with 5 M GSK3484862, 1 M AM580, and 1 g/mL doxycycline. A media change was performed every other day. After a total of seven days of differentiation, cells were harvested with Accutase and sorted based on reporter expression. Cells were fixed using a Parse fixation kit, and scRNAseq library preparation was performed using a Parse WT Mega v2 kit. A list and description of samples is provided in Supplementary Table 6.