RNA-Seq of fat body (replicate 2) from Achroia grisella (Lesser Wax Moth)
The RNA extractions were stabilized and prepared using GeneWiz RNA stabilization tubes (Cat. #: GTR5025-GW; Azenta Life Sciences, China), as per the manufacturers protocol, and subsequently provided to Azenta Life Sciences for commercial RNA sequencing. RNA-Seq libraries were prepared using poly(A)-mRNA selection and strand-specific protocols with unique dual indexing. Sequencing was performed on an Illumina NovaSeq 6000 platform using S4 flow cell lanes, generating paired-end reads (2150 bp).