LHCN-M2 differentiated + basal rep 3 GFP total mRNA
LHCN-M2 cells were differentiated for 6 days, infected with an MPRA lentivirus, and collected 2 days later. Total RNA was isolated from frozen lysates using the Qiagen Allprep mini kit. rRNA depletion, polyA selection, and library preparation were performed using the NEBNext Ultra II Directional RNA Library Prep kit.