scRNA-seq of HCC38 Vehicle
scRNA-seq libraries were prepared from HCC38 cells chronically treated with vehicle or nicotine following the 10X Genomics Single Cell 3 V2 Reagent Kits. cDNA from the reverse transcription reaction was purified using DynaBeads MyOne Silane Beads. The libraries were then fragmented, end-repaired and A-tailed, amplified and indexed with unique sample index i7 through PCR amplification, then sequencing on a NovaSeq 6000.