DNA raw reads of Vigna angularis: Baoqing variety
For Library preparation, 5 g of high-molecular-weight genomic DNA of each library sample was fragmented to a size distribution mode between 15 kb - 20 kb using a 2-cycle shearing method on the Megaruptor 3 system. Single-strand overhangs were removed by enzyme digestion. Single-strand overhang digestion was followed by DNA damage repair and End repair/A-tailing. DNA damage was repaired using the PacBio DNA Damage Repair Mix v2. End repair/A-tailing was done using the PacBio DNA Damage Repair Mix v2. Next, 5L of PacBio Overhang Adaptor V3 was added to 60.0 L - 68.0 L of each reaction mixture for Adaptor mix ligation. The SMRTbell Library samples were then purified using 1.0X AMPure PB Beads. Nuclease mix was added to the SMRTbell library samples to remove damaged SMRTbell templates. For HiFi sequencing, the PippinHT system (Sage Science) was used for size selection of SMRTbell library samples. The size-selected HiFi library fractions were further purified with 1.0X AMPure PB Beads before sequencing with the PacBio Sequel IIe.