Double digest Genotyping by Sequencing (GBS) library of Cucurbita ficifolia from Ocotln, Morelos (F_MOROco_2)
The seed was germinated in a greenhouse and total DNA was extracted from fresh leaves using a CTAB protocol. The extracted DNA was sequenced by University of Minnesota Genomics Center, where two restriction enzymes (NspI and BfuCI/Sau3AI) were used, with fragment size selection at 200-300 base pairs. Genotyping by Sequencing (GBS) method was performed with an Illumina Novaseq instrument (1 x 100 bp, SE).