DMSO-treated control of inducible UNG-KO Plasmodium falciparum, second cycle after knock-out, schizont parasite genomic DNA sample, after U-DNA-IP
U-DNA-IP sample for U-DNA-sequencing. Genomic DNA from double sorbitol synchronized, schizont stage parasites was isolated after 44-hour of DMSO-treatment as mock control for UNG-KO parasite strain. Genomic DNA was fragmented for a target of 200-400 bp-long DNA fragments. Fragmented DNA was incubated with catalytically inactive FLAG-tagged UNG sensor and pulldown using anti-FLAG beads. Library was prepared using NEBNext Ultra II DNA Library Prep Kit.