WGS of Robinsoniella peoriensis: Pig Manure Isolate
DNA samples were prepared for whole genome sequencing using the Oxford Nanopore Technologies SQK-NBD114 native barcoding kit. Long Fragment Buffer was used to promote longer read lengths. Sequencing was performed on the GridION platform using a FLOW-MIN114 Spot-ON Flow Cell, R10 version with a translocation speed of 400bps. Fast5 were basecalled with the GridION using the super-accurate basecalling model (Guppy v7.0.9) and files were concatenated into one fastq.