WGS of orthoreovirus EL1562
RNA was extracted using Zymo quick-DNA/RNA miniprep plus kit (ZymoBIOMICS) with DNase 1 digestion. RNA was denatured at 98C for 10 minutes, followed by cooling at 4C for 1 minute. cDNA synthesis was carried out using Superscript IV First-strand Synthesis System (Thermofisher), followed by NEBNext Ultra II Non-Direction RNA Second Strand Synthesis module (NEB). Sequencing library was prepared using Nanopore Native Barcoding Kit 96 V14. Library was loaded onto a R10.4.1 flowcell and sequenced using the Nanopore Mk1C. Sequence data was base called using Dorado (0.8.2) with super accurate base-calling.