Single-cell multiome sequencing data for CLL patients relapsed on BTK inhibitors
The single-cell nuclei suspension was generated following 10X genomics protocol (CG000365) for Nuclei Isolation for Single Cell Multiome ATAC + Gene Expression Sequencing. Lysis of CLL cells was optimized to 40s on ice. Next, we prepared the libraries for sequencing by following 10X genomics protocol (CG000338, Rev F) 10X Genomics Chromium Next GEM Single Cell Mulitiome ATAC + GEX Protocol, with a target capture of 10,000 nuclei/sample. Libraries were sequenced on an Illumina NovaSeq 6000 system using Illumina kit following manufacturers instructions.