S07003_d9_1
Frozen nuclear pellets of 5x10^4 cells each were thawed on ice, suspended in 50uL transposition reaction mix (2.5uL Tn5 transposase in 1x TD buffer, Illumina Cat# FC-121-1030), and incubated for 30min at 37C. Reactions were purified using Qiagen MinElute kit, eluted in 10uL water and amplified using the KAPA real-time library amplification kit (KAPA Biosystems) with barcoded adaptors. PCR reactions were terminated after 10-13 cycles and purified using AmPure XP beads (Beckman Coulter). Samples were size selected using SPRIselect beads (Beckman Coulter) to a size range of 150-850 kbp and sequenced on an Illumina HiSeq2500 to an average depth of 30 M 100bp paired-end reads.