ITS sequencing results from Lutter biosolids
The internal transcribed spacer (ITS2) regions of the fungal 18S rRNA gene was amplified using a two step approach using the locus specific primer pair ITS3 (5-GCATCGATGAAGAACGCAGC-3) and ITS4 (5-TCCTCCGCTTATTGATATGC-3) with 20 PCR cycles for the first step and 15 PCR cycles for the second step.