miRNA-Seq of Homo sapiens: AD01 plasma exosome
RNA was extracted from plasma exosomes, purified, and enriched for small RNA fragments of 18-30 nt. The 3' and 5' ends were ligated, reverse transcribed into cDNA, and amplified by PCR. The PCR products were recovered and purified, and then cyclized to form a single-stranded circular DNA library.