Single-cell TCR sequencing of T cell cultures derived from FLC TILs
TILs were expanded ex vivo from primary tumor core needle biopsies from patient SJ1 according to Dudley et al, J Immunother, 2003. After expansion, SJ1 TILs were stimulated with DNAJB1-PRKACA fusion peptide EIFDRYGEEV (1 uM). After stimulation, live CD8+ cells were isolated by FACS and subjected to scTCRseq using the 10x Chromium platform using the Single Cell 5 V(D)J v1 kit.