slc2a10 KO pool 3
each experiment consists of 5 pools containing each 30 mutant (KO or KI) zebrafish embryos and were compared against 5 pools of 30 wild type control cousins. All RNA extractions were performed at 5 dpf. The RNeasy Mini kit (Qiagen, Hilden, Germany) was used according to manufacturers guidelines. Next generation sequencing libraries were generated following manufacturers procedures (TruSeq Stranded Total RNA kit, Illumina, San Diego, USA) and sequenced on a NovaSeq6000 instrument (Illumina)