RNA-seq of Sorghum bicolor developing grain
For this study, grains were collected at 10 stages of grain developments. Samples were then frozen in liquid nitrogen and stored at -80C till extraction. This protocol was carried out for each developmental stage and in triplicate. Total RNA were extracted following instructions of the kit (RNAzol Sigma), and their quantity and quality were evaluated (4200 Tapstation, Agilent Technologies).