small RNA-seq of Spodoptera exigua: female head and thorax
Tissue dissection from adult females was performed as described above whereas in the case of the Sf21 cells, we recollected all cells from a 25 ml flask at 80% confluence. RNA extraction and DNase treatment were performed as described above. DNase-treated total RNA was sent to Macrogen (Korea), where libraries were prepared using the TruSeq Small RNA Library Preparation Kit (Illumina), following the manufacturers instructions. Libraries were sequenced on an Illumina HiSeq X platform, producing 150 bp paired-end reads.