RNA-Heavy metal-microplastic complexes contaminate soil
Soil DNA was extracted using a rapid DNA kit for soil, and the concentration and purity of the extracted DNA was determined using a 1% agarose gel and fluorometer. Primers were used to amplify the bacterial 16S rRNA gene using the high variant region V3-V4. After amplification, library size is measured by agarose gel electrophoresis and library concentration is determined by fluorometric quantification. Purified amplicons were pooled in equimolar amounts for paired-end sequencing using the Illumina MiSeq PE300 platform. Raw 16S rRNA gene sequencing reads were demultiplexed with fastp version 0.20.0, quality filtered, and combined with FLASH.