RNA-seq of cancer cells in the low and high cellular densities.
To compare and analyse the gene expression changes of cancer cells in the low and high cellular densities. MCF10A cell line was transduced with Tet-On inducible pLV lentiviral vector for HER2_GFP. Her2 is the rat WT Her2 and all cells were treated with 1 uM Doxycyclin. Celle were cultured in LD (500 cell/cm2) or high density (20000 cell per cm2) for 72 hours.