Shotgun metagenomic analysis of nettle fertilizer; replicate 2
Extraction for shotgun metagenomic profiling was done using a MP Biomedicals FastDNA SPIN Kit for Soil and a FastPrep 5G instrument. Metagenomic DNA was fragmented and sequencing libraries constructed using an Ion Xpess Plus Fragment Library kit, with fragmentation timings optimized to generate read inserts of 150 bp. Fragment profiles were confirmed on an Agilent Bioanalyzer. The replicates were each sequenced on separate 540 chips using an Ion S5 sequencer with the addition of an external calibration standard to generate high quality sequencing data for subsequent analysis.