Narcissus pseudonarcissus: Whole Genome Shotgun: Capanne di Marcarolo
Fresh leaves of N. pseudonarcissus were collected from a single individual in a wild population. Leaveswere dehydrated using silica-gel as described in Chase and Hill, 1991. DNA was extracted by using the Macherey-Nagel Plant II kit, following manufacturers suggestions. Purified DNA was then sequenced by Macrogen (Amsterdam, The Netherlands) using a Illumina TruSeq Nano DNA kit (350bp insert) on a Illumina NovaSeq X machine, obtaining 2x150 paired end libraries.