RNA-seq of GOT1 cells: ctrl D1 - sample 001
Total RNA isolated using the RNeasy Mini Kit (QIAGEN) according to the manufacturers protocol and stored at -80C before further processing. RNA concentration, purity and quality were assessed on a Nanodrop 2000 Spectrophotometer (Thermo Scientific) and Bioanalyzer 2100 (Agilent). Library preparation and RNA sequencing were performed at Genomescan B.V. (Leiden, The Netherlands). In short, libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs). Clustering and DNA sequencing on the NovaSeq6000 (Illumina) was performed at an average sequencing depth of 28 million reads and 150 base pair read length.