RNA Seq of Patient 25
Bacterial 16S rRNA sequencing was performed on DNA extracted from FFPE PCa and BPH tissues using QIAGEN kit, amplified using target-specific primers and the Roche FastStart High Fidelity PCR System, purified, barcoded, and sequenced on the Ion S5 XL platform, with downstream analyses conducted using DADA2, Phyloseq, and PICRUSt2 for taxonomic and functional profiling.