RNA-Seq of mus musculus: intestinal organoids
Intestinal crypts from Vil-creERT2;Rank fl/fl mice were cultured and pretreated with DMSO or 4-OHT for 3 days. Then, the intestinal organoids were passaged and treated with 20ng/ml IL-13 and with or without 50ng/ml RANKL for 4 days. Finally, the intestinal organoids were harvested and total RNA was isolated with TRIzol reagent. Library preparation and sequencing were performed by BGI Genomics Co.,Ltd. Samples were sequenced on BGISEQ platform.