Sequencing of Pooled Genomic DNA from Chlorovirus Isolates NE-41-2-m, OSyNE-5A-L1, and NES-5A-S1
Sequencing of pooled genomic DNA from three distinct chlorovirus isolates via PacBio Sequel circa 2019. DNA isolation from purified virus was done using Qiagen Genomic-tip 100/G; the genomic DNA from each isolate was then pooled in equal amounts. Each of the three individual whole genome sequences can be assembled from this pooled sequence data and are sufficiently different from each other that their virus type can be determined by GC content and comparison to reference chloroviruses. For the description of a previous project that also utilized sequencing of pooled chlorovirus genomic DNA, see Jeanniard et al. 2013 (doi:10.1186/1471-2164-14-158); this sequencing was done in a similar manner.