WGS of R84105
Cells were harvested as a full loop of a fresh culture on ISP4 medium in 300 L sterile MQ and 900 L 100% ethanol. Seventy-five L of the enzyme mix (3 mg lysozyme, 20 L Mutaloenzyme, 55 L TES buffer per sample) was added to the samples, which were vortexed and incubated at 37C for 1 hour. The samples were centrifuged twice at 4C for 3 minutes each at maximum speed, discarding the supernatants. DNA was extracted using the Maxwell RSC machine (Promega). To the sample, 300 L of GIT buffer was added, and it was vortexed to achieve a homogeneous solution. Following this, 300 L of the sample was added to the Maxwell cartridge, and 200 L of Tris-HCl was added to the elution tubes, where DNA was collected. Two L of RNAse was then added to the extracted DNA, vortexed, and incubated at 37C for 1 hour. The extracted DNA was diluted in Tris-HCl to a concentration of 40 ng/L, and 80 L of this solution was sent for Illumina whole genome sequencing (outsourced to Oxford Genomics Center). The raw files of the Streptomyces sp. 7H genome were analyzed using the PATRIC comprehensive genome analysis online service. The genome was assembled using the auto (unicycler) method and annotated using the RAST (Rapid Annotation using Subsystem Technology) tool kit (RASTtk) (Wattam et al., 2017).