Single-cell gene expression sequencing of unstimulated T cells from ascites
TILs were expanded ex vivo from ascites fluid from patient SJ3 according to Dudley et al, J Immunother, 2003. After expansion, SJ3 TILs were stained with human hashtag/barcoded antibodies to distinguish between separate TIL wells. TILs were then subjected to scRNAseq using the 10x Chromium platform using the Single Cell 5 V(D)J v1 with Feature Barcoding kit. Antibody barcode identities include HHash1_TotalC0251 with sequence GTCAACTCTTTAGCG, HHash2_TotalC0252 with sequence TGATGGCCTATTGGG, HHash3_TotalC0253 with sequence TTCCGCCTCTCTTTG, HHash4_TotalC0254 with sequence AGTAAGTTCAGCGTA