RNA-seq of homo sapiens: PBMC derived activated T follicular helper CD4+ T cells
RNA-seq samples were generated from ~5000 FACS sorted cells. Takara ssv4 Ultra low input kit was utilized and cellx were immediately washed, pelleted and then prcoessed to cDNA using kit instructions. Libraries were prepared using Nextera DNA assay and Nextera Index kits. Libraries were cleaned with either a Zymogen clean and concentrate kit or using Ampure XP beads or both befoe they were measure, normalized, and sequenced.