RNA-seq of Setaria viridis A10 poly A rep 1
RNA from c. 100mg of leaf material was extracted with an RNeasy Plant Mini Kit (74904 Qiagen) and libraries were prepared by using NEB Kit E6440S. Sequencing of the ribo-depleted and poly-A libraries, each with three biological replicates, were performed using Illumina NovaSeq 6000 (Genomic Centre, Queen Mary University of London), generating 150 bp paired-end reads.