RNA-Seq of ATCC43300(MRSA)
Take the Total RNA Extractor (Trizol) extraction kit as an example. For customers who bring their own RNA, this can be ignoredPartial content1. Let the lysed sample or homogenate stand at room temperature for 5 to 10 minutes to ensure complete separation of nucleoproteins and nucleic acids.2. Add 0.2ml of chloroform, shake vigorously for 15 seconds, and let it stand at room temperature for 3 minutes. Centrifuge at 12,000 RPM and 4C for 10 minutes.3. Draw the upper aqueous phase and transfer it to a clean centrifuge tube. Add an equal volume of isopropyl alcohol, mix well, and let it stand at room temperature for 20 yearsmin.Centrifuge at 12,000 RPM and 4C for 10 minutes, then discard the supernatant.5. Add 1ml of 75% ethanol to wash the precipitate. Centrifuge at 12,000 RPM and 4C for 3 minutes, then discard the supernatant. Room temperature drying5 to 10 minutes.6. Add an appropriate amount of RNase-free ddH2O to fully dissolve the RNA. Place the obtained RNA solution at -70CSave or use immediately for subsequent tests.