PacBio sequencing of rRNA using universal eukaryotic primers ITS9mun-ITS4ngsUni: seawater metagenome
DNA extraction: Macherey-Nagel Nucleospin Plant II Mini; PCR primers: ITS9mun and ITS7ngsUni; PCR purified using FavorPrep GEL/PCR Purification DNA fragments Mini Kit (Favorgen).