Single-cell TCR sequencing of unstimulated T cell cultures derived from FLC TILs
TILs were expanded ex vivo from tumor fragments from patient SJ2 according to Dudley et al, J Immunother, 2003. After expansion, SJ2 TILs were stained with human hashtag/barcoded antibodies to distinguish between separate TIL wells. TILs were then subjected to scTCRseq using the 10x Chromium platform using the NextGEM Single Cell V(D)J v1.1 with Feature Barcoding kit. Antibody barcode identities include HHash1_TotalC0251 with sequence GTCAACTCTTTAGCG, HHash2_TotalC0252 with sequence TGATGGCCTATTGGG