16S rRNA gene metabarcoding of Lontra longicaudis feces: collected at Burro site on Feb_2020
Genomic DNA was extracted using the QIAamp DNA Stool Mini Kit (QIAGEN) following the manufacturers protocol. DNA concentration and purity were assessed via spectrophotometry with a NanoDrop 2000 (Thermo Fisher Scientific). The amplifiability of the V4 region of the 16S rRNA gene was evaluated by PCR using primers 515Frcbc47 (5'-TCGAAGTATGGTAATTGTGTGYCAGCMGCCGCGGTAA-3') and 806R (5'-AGTCAGCCAGCCGGACTACNVGGGTWTCTAAT-3'). DNA samples that successfully amplified the target fragment were sent to Novogene Corporation Inc. (California, USA) for bacterial 16S rRNA gene amplicon sequencing. Sequencing was performed using primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') on an Illumina NovaSeq 6000 platform with 2  250 bp paired-end reads.