Metagenome sequencing: Fosmid Clone NIOT F42
The Fosmid clone DNA was end-repaired (NEBnext ultra II end repair kit, New England Biolabs, MA, USA), cleaned up with 1X AmPure bead. Native barcode ligation was performed with NEB blunt/ TA ligase and barcode ligated DNA sample was adapter ligated for 15 minutes using NEBNext Quick Ligation Module. Library was cleaned up using 0.6X AmPure beads and finally sequencing library was eluted in 15 l of elution buffer and sequenced on Nanopore PromethION system