Long-Read Genome Sequencing of Tetraploid Wheat Kronos Using PacBio Revio
High molecular weight (HMW) genomic DNA was extracted from 5 g of juvenile leaf and immature inflorescence tissue using the Nanobind Plant Nuclei Big DNA Kit. The integrity and size of the extracted HMW DNA were assessed using the Femto Pulse system with the Genomic DNA 165 kb Kit. Two HiFi long-read sequencing libraries were constructed from 30 g of genomic DNA. Sequencing was performed on six PacBio Revio 25M SMRT cells at the DNA Technologies and Expression Analysis Core Facility, University of California, Davis.