RNA-seq of A3B-off gefitinib-resistant PC9 cell line E
RNA was isolated from cells using RNeasy columns (Qiagen). RNA-sequencing was performed by Novogene. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dTTP for non-directional library. Libraries were subjected to end-repair, A-tailing, adapter ligation, size selection, amplification, and purification. Quantified libraries were pooled and sequenced on NovaSeq PE150.