Far-red light - Day 3 - sRNA - Replicate 1
Tissue samples were homogenized using 3 mm tungsten carbide beads in a TissueLyser II (Qiagen). Total RNA was extracted using the Maxwell 16 LEV Plant RNA Kit and the Maxwell 16 Instrument (Promega). RNA quality and quantity were assessed using a combination of NanoDrop (Thermo Fisher Scientific), Qubit (Thermo Fisher Scientific), and Experion (Bio-Rad). Library preparation and sRNA sequencing (NovaSeq 6000 SE150) were performed by Novogene.