snRNA-Seq of mus musculus:sample2_01
The prepared single-cell suspension was subjected to quality control and counting, with requirements including a cell count  50,000, cell viability  80%, and minimal background impurities. Qualified cells were resuspended to an appropriate concentration of 5001200 cells/l for subsequent processing on the 10x Genomics Chromium system.Based on the expected number of target cells, the appropriate number of cells was loaded to generate Gel Bead-In-EMulsions (GEMs) for single-cell isolation. After successful GEM formation, the GEMs were collected, and reverse transcription was performed in a PCR instrument to achieve barcoding. The GEMs were then broken to release the contents, and first-strand cDNA was purified and enriched using magnetic beads, followed by cDNA amplification and quality control.Qualified cDNA was used to construct second-generation sequencing libraries through processes including fragmentation, sequencing adapter ligation, and sample Index PCR. Finally, the libraries were quantitatively assessed for quality.The completed libraries were sequenced using the Illumina NovaSeq 6000 platform with a PE150 sequencing mode, achieving a sequencing depth of 20,000 reads per cell or higher.