SMMseq of shMT2A CAOV3 cells passaged with low dose cadmium
Cells were collected from a 10cm culture plate, pelleted, and genomic DNA isolated by Invitrogen PureLink Genomic DNA Mini Kit. NEB Alu I and Mlu CI were used to digest genomic DNA. AMPure XP beads were used to purify DNA. NEB NEBNext Ultra II DNA Library Prep Kit for Illumina was used with custom P5_HP6N adapter. After end-filling, rolling circle amplification was performed and then PCR amplified with Illumina P5 and P7 adapters using NEBNext Ultra II Q5 Master Mix. AMPure XP beads were again used to capture DNA.