Single-cell TCR sequencing of unstimulated CD8+ T cells from PBMCs
PBMCs from patient SJ1 were stained with a DNA-barcoded, fluorescently-labeled A*68:02-EIFDRYGEEV tetramer to label fusion-specific T cells. Live, CD11b-, CD19-, CD8+ cells cells were isolated by FACS and subjected to scTCRseq using the 10x Chromium platform using the NextGEM Single Cell V(D)J v1.1 with Feature Barcoding kit. Antibody barcode identities include PEstrep_TotalC0954 (bound to the tetramer) with sequence TATTTCCACCCGGTC.