RNA-Seq of PK15-ANXA1KO cells+PAstV
Sequencing was performed on an Illumina platform using paired-end (PE) Next-Generation Sequencing (NGS). Raw sequencing data underwent quality filtering to yield clean data, which was subsequently aligned to the species reference genome. Gene expression levels were quantified based on the alignment results. Downstream analyses included differential gene expression analysis, functional enrichment analysis, and sample clustering. Additionally, aligned reads were assembled to reconstruct transcript sequences.