WGS of pseudomonas aeruginosa
Bacterial isolates were cultured overnight in 2ml LB media, followed by DNA extraction using the PureLink Microbiome DNA Purification Kit. DNA purity was assessed with Nanodrop, and concentration was measured using Qubit dsDNA HS kit on a Qubit 4.0 fluorimeter. Library preparation was performed using the Nanopore Rapid Barcoding Kit (SQK-RBK110.96), where DNA was fragmented and barcoded using transposase enzyme in a PCR-free step. Samples were pooled, purified with AMPure XP Magnetic Beads, and adapters were ligated for sequencing on a MinION Mk1C device.