Bulk RNA-seq of monocytes isolated from healthy control
Bulk RNA-seq were conducted with an Illumina Novaseq 6000 (Illumina, San Diego, CA, USA), which generated paired-end (PE) reads of approximately 100 bp in size. According to the instruction of the NEBNext Ultra RNA Library Prep Kit (Illumina, San Diego, CA, USA), a sequential process of end-repair, poly-A addition, and adaptor ligation on both ends was carried out.