Long-read PacBio DNA-seq of Phytophthora medicaginis isolate 7831
Genomic DNA was sheared, then 10 Kb(20 Kb if the genome size larger than 30M) double-stranded DNA fragments was selected. DNA fragments were end repaired and ligated with universal hairpin adapters. Subsequent steps were followed as per the manufactures instruction to prepare SMRTbell library. The library was sequenced in PacBio RSII/Sequel SMRT instrument.