RNA-Seq of mus musculus : splenic Treg cells
Two thousand live FoxP3+ Treg and FoxP3- Tconv cells were sorted by flow cytometry. Total RNA was then reverse-transcribed and amplified into cDNA libraries using the Smart-Seq2 protocol (Illumina). All libraries were then sequenced on an Illumina NovaSeq X Plus platform using 150 bp paired-end reads (2  150 bp).