Ribo-seq of mus musculus: adult DRG
1. Using translation inhibitors, ribosomes undergoing translation are fixed on the mRNA sequence or initiation sites. 2. Lyse cells, add RNase, and digest mRNA not protected by ribosomes. 3. Separating individual ribosomes and extracting undigested short fragment mRNA from the purified ribosomes; 4. Construct the library and perform sequencing on the machine.