IsoLoop metagenome
DNA was extracted using the PowerSoil Pro DNA Isolation Kit (Qiagen, Hilden, Germany) with high-throughput automation on the QIAcube HT platform (Qiagen, Hilden, Germany). Mechanical lysis was achieved via bead beating using PowerBead Pro plates (Qiagen, Hilden, Germany), which contain a mixture of 0.5 mm and 0.1 mm ceramic beads to ensure efficient disruption of microbial cells. Extracted genomic DNA was quantified using the Quant-IT PicoGreen dsDNA Assay Kit (Invitrogen, Waltham, MA). Library preparation was carried out using a proprietary protocol adapted from the Nextera XT kit (Illumina, San Diego, CA).