MN(VII) filter 7cm
DNA extraction using the DNA isolation kit (Omega, cat: M5635-02), barcoded PCR primers set Vn F (5-ACTCCTACGGGAGGCAGCA-3) and Vn R (5-GGACTACHVGGGTWTCTAAT-3) was used to amplify the V3-V4 region of the 16S rRNA gene