CP genomic sequences
High-quality total genomic DNA was extracted from Tortula acaulon sample using a modified CTAB protocol, ensuring the removal of polysaccharides and secondary metabolites. DNA integrity was assessed using Nanodrop, Qubit fluorometry, and agarose gel electrophoresis.For short-read sequencing, libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit, targeting an average insert size of ~350 bp. Fragmentation was performed enzymatically, followed by adapter ligation and PCR amplification. The final libraries were quantified using a Qubit dsDNA HS Assay Kit and assessed for size distribution with a Bioanalyzer (Agilent 2100).Sequencing was conducted on an Illumina NovaSeq 6000 platform using 150 bp paired-end reads, generating high-depth coverage for downstream hybrid genome assembly, polishing, and variant calling.