ITS amplicon sequencing of concrete metagenome
To characterize bacterial and fungal communities, unsaturated PCR amplification of 16S and ITS rDNA fragments was performed using the Q5 High-Fidelity DNA Polymerase [New England Biolabs, Cat. No. M0491], which provides ultra-low amplification error rates. For fungi, the ITS1 region was amplified using primers ITS5-1737F (5-GGAAGTAAAAGTCGTAACAAGG-3) and ITS2-2043R (5-GCTGCGTTCTTCATCGATGC-3).