Bos taurus genome Pumpo
Following the established protocols of Illumina (Illumina, San Diego, CA, USA), a genomic library configured for paired-end sequencing (2  150 bp) was prepared and sequenced using an Illumina Novaseq 6000 system by GENEWIZ (South Plainfield, NJ, USA). The sequencing library was clustered on a flowcell, which was installed in the Illumina sequencing machine according to the manufacturers instructions. The Illumina Control Software conducted image analysis and base calling. The raw sequencing data (.bcl files) obtained were converted into fastq format using Illuminas bcl2fastq 2.17 software, which allows for a single mismatch during index sequence identification.