16S rDNA amplicon sequencing of a Moving bed biofilm: 60% O2
The biofilms were scraped into phosphate buffer solution at reactor shutdown. Biomass samples were centrifuged at 12,500 g for 10 min and pellets were stored at 20 C until DNA extraction. DNA was extracted using the FastDNA SPIN Kit for Soil (MP Biomedicals, OH) by following the manufacturer's protocol. The extracted DNA was sent to BGI Genomics Hong-Kong that ran 16S rDNA sequencing. V3-V4 hypervariable region of the 16S rRNA gene was amplified by PCR with the universal primer set 341F (5-ACTCCTACGGGAGGCAGCAG-3) and 806R (5-GGACTACHVGGGTWTCTAAT-3). DNBSEQ platform generated 2x 47000 300-bp paired-end reads for each sample.