RNA-seq of S1B del_mcgB in Chemically defined media with glucose: Rep2
Three biological replicates of S. mutans Smu102 wildtype and mcgB were grown for 14 hours in nonselective CDMB with 1M glucose. RNA was extracted in biological triplicate with TRIzol (Thermo-Fisher, San Jose, CA) according to the manufacturers instructions. Sample purity was assessed by DNA-dependent amplification of ldh using PCR. Sample cleanup was performed using a Monarch Total RNA Miniprep Kit (New England Biolabs, Ipswich, MA, USA). Then, the mRNA was fragmented, cDNA was reverse transcribed with random hexamer primers, and the library was prepared and sequenced on Illumina Novaseq platform with the paired-end mode and read length of 150bp. Approximately 2Gb of raw reads were generated for each sample.