Single-cell TCR sequencing of CD8+ T cells from PBMCs
PBMCs from patient SJ1 were stained with a DNA-barcoded, fluorescently-labeled A*68:02-EIFDRYGEEV tetramer to label fusion-specific T cells. Live, CD11b-, CD19-, CD8+ cells cells were isolated by FACS and subjected to scTCRseq using the 10x Chromium platform using the Single Cell 5 V(D)J v1 with Feature Barcoding kit. Antibody barcode identities include PEstrep_TotalC0954 (bound to the tetramer) with sequence TATTTCCACCCGGTC.