Mesenchymal Stromal Cells from Human Adipose Tissue
Adipose tissue-derived MSCs were derived from cells obtained during abdominal plastic surgery and stored in the Cell Culture Collection of the N.K. Koltzov Institute of Developmental Biology RAS (Moscow, Russia). The cells were cultured in DMEM, 10%FBS, 1% GlutaMAX, 1% penicillin/ streptomycin, 1% ITS (Gibco, USA). One million cells were collected, and total RNA was extracted using the RNeasy Mini Kit (Qiagen, Germany). The library was prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, USA).