RNA-Seq of Homo sapiens: renal tubular epithelium HK-2 treated with Opisthorchis viverrini #2
Total RNA was extracted from cultured cells using the easy-spin (DNA free) Total RNA Extraction Kit (iNtRON Biotechnology, Korea) following the manufacturers protocol. High-quality RNA was selected for library construction using the TruSeq Stranded mRNA Library Prep Kit (Illumina), which employs poly(A) selection and strand-specific preparation. Libraries were sequenced on an Illumina platform using paired-end 2  151 bp reads.