Mixed_sludge_january_RNA
Extractions were performed using ZymoBIOMICS DNA/RNA Miniprep Kit (R2002, Zymo Research, Irvine, CA, USA). The libraries for metatranscriptome sequencing were prepared using the Zymo-Seq RiboFree Total RNA Library Kit, which includes a universal rRNA depletion step. Paired-end mRNA sequencing was then performed on a NextSeq 550 sequencer (Illumina) using the NextSeq High Output Kit v2 Sequencing Reagent Kit.