ZXH-P-1-3
Total RNA was extracted using a mirVana miRNA Isolation Kit (Ambion-1561; Ambion, Inc, Austin, TX, USA) following the manufacturers protocol. RNA integrity was evaluated using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), and samples with an RNA integrity number (RIN)  7 were used in subsequent analysis. DNA libraries were constructed using a TruSeq Stranded mRNA LT Sample Prep Kit (Illumina Inc., San Diego, CA, USA) according to the manufacturers instructions. These libraries were then sequenced using the Illumina HiSeq 4000 sequencing platform, which generated 125/150-bp paired-end reads.