ONT nanopore (1D standard) of Clarias macrocephalus (CM): adult male (M) liver
For library preparation of standard ONT long noisy reads, we used 1D fragmented or size-selected pooled libraries of end-polished, nick-repaired, and A-tailed DNA fragments produced by ligating sequencing adapters onto double-stranded DNA fragments and purified with AMPure XP beads. The library was assessed with QuBit and sequenced on a Nanopore PromethION system (NovoGene). The standard ONT sequencing library yielded a total of 57 Gb of data, achieving a depth of 36X in genome coverage, with a read N50 of 13-kilobase (kb). Sequencing data Phred or quality (Q) scores are integer value representing the estimated probability of an error (i.e., that the base is incorrect) and if P is the error probability, such as P = 10-Q/10  and Q = -10 log10(P) we estimate ONT data  median read quality of Q11.03 and 97.0% of reads with quality >Q7 (2,023,582 reads)