Sequencing results after PRESTO-Salsa screening of HumanMetabolite_644
Human metabolites were screened against PRESTO-Salsa reporter cells (HEK293T) co-transfected with PRESTO-Salsa reporter plasmids and PRESTO-Tango plasmids for a high throughput assessment of GPCR activation. Each reporter cell monoclonally expresses one of 314 conventional G-protein coupled receptors (GPCR) and contains a reporter plasmid that expresses a unique barcode when the GPCR is activated. This unique barcode was then mapped to its corresponding GPCR via next-generation sequencing to simultaneously assess nearly all conventional GPCRs in one screening.