V3-V4 Metagenome of Pulmonary TB Patients - NER16
The fragments of the V3V4 regions of the 16S rRNA gene fragments were amplified using the primers, the PCR product was mixed with an equal volume of 1X loading buffer (contained SYB green), and electrophoresis was performed on 2% agarose gel for detection. The amplicon was then purified again with AMPure XP bead.