16S sequencing results from Montiers biosolids
V3 and V4 regions of the bacterial 16S rRNA gene were amplified using a two step approach with the locus specific primer pair 341F (5-CCTACGGGNGGCWGCAG-3) and 805R (5-GACTACHVGGGTATCTAATCC-3) with 20 PCR cycles for the first step and 10 PCR cycles for the second step.