exome sequencing of human mammary adenocarcinoma cell line T47D resistant to Palbociclib and Fulvestrant treatments
DNA was extracted using QIAamp DNA Mini Kit (Qiagen). DNA exome sequencing were performed using Twist Comprehensive Exome Panel according to the manufacturers instructions. The sequencer used is a NovaSeq6000 (Illumina). The sequencing was performed using a S4 V1.5 cartridge (Illumina), 2 x 150 bp. A mean of 150M of reads were obtained for each sample, assuring a minimum of 30X coverage.