salami matrix sample A23
Shotgun metagenomics sequencing (paired-end 150 bp, 9 Gb per samples) was performed on Illumina Novaseq machine by Novogene company (Cambridge, United Kingdom). After Qubit 2.0 quantification, 1 g of DNA was used for library preparation using NEBNext library prep Kit, randomly fragmented and then polished, A-tailed, and ligated with the NEBNext adapter for Illumina sequencing, and PCR enriched. The purification PCR products was performed with AMPure XP system. The libraries were analysed by Agilent 2100 Bioanalyzer and quantified using real-time PCR. The reads were quality filtered with Solexa QA++ software, sequences below 60 bp and dereplicated sequences were removed by Prinseq.