Small RNA sequencing of Eucalyptus tereticornis
Approximately, 1 g of total RNA was used for library construction using the NEBNext UltraTM II RNA Library Prep Kit for Illumina (New England Biolabs, MA, USA) following the manufacturers protocol. The ready to run final library was quantifed using Qubit 3.0 fuorometer (ThermoFisher Scientifc, USA), following the manufacturers protocol. The insert size of the library was determined using 2100 Bioanalyser (Agilent Technologies, USA) and paired-end sequencing was performed on Illumina HiSeq 2500 (Illumina, San Diego, CA, USA) with a read length of 150 X 2 bp.