16S rRNA sequencing of pig mid-colonic digesta
Genomic DNA was extracted from pig mid-colonic digesta using the NucleoSpin Soil Kit with modifications. The V3V4 region of the 16S rRNA gene was PCR-amplified using universal primers 5-TACGGGAGGCAGCAG-3 and 5-CCAGGGTATCTAATCC-3. Libraries were sequenced on the Illumina MiSeq platform with V3 chemistry and 2300 bp paired-end reads.