Shotgun metagenomics of the human gut microbiome
DNA extracton and sequencing were performed by Macrogen Japan Corp. (Tokyo, Japan). Briefly, for library construction, DNA/RNA is extracted from a sample. After performing quality control (QC), qualified samples proceed to library construction. The TruSeq DNA PCR-Free kit (Illumina, Inc. San Diego, CA USA) was used as the Library Kit. The Illumina platform (Illumina, Inc. San Diego, CA USA) was used for sequencing.