16S rRNA (V1-V3) sequencing: nasal swabs from experimental human SARS-CoV-2 infection
DNA extraction with Powersoil Pro kits (Qiagen), library preparation with Powersoil Pro kits, V1-V3 library preparation (27F, 534R), and sequencing on NextSeq 1000 instrument (Illumina) configured for 300-bp PE reads.