snATAC_PPC_Pool1_R01L01S01
Detailed methods are found in doi 2021.10.20.465206. Nuclei from two pools of cells were isolated according to the manufacturer's recommendations (Manual CG000169, Rev B), transposed, and captured as independent samples according to the manufacturer's recommendations (Manual CG000168, Rev B). All single nuclei were captured using the 10x Chromium controller (10x Genomics) according to the manufacturer's specifications and manual (Manual CG000168, Rev B). Cells for each sample were loaded on the individual lane of a Chromium Chip E. Libraries were generated using Chromium Single Cell ATAC Library Gel and Bead Kit (10x Genomics) following manufacturer's manual (Manual CG000168, Rev B). Sample Index PCR material was amplified for 11 cycles. Libraries were sequenced using a custom program (50-8-16-50 Pair End) on HiSeq 4000.