Bacterial WGS of Burkholderia pseudomallei isolated from a soil sample.
Genomic DNA extraction was performed on an overnight culture of Burkholderia pseudomallei using QIAamp DNA mini kit. Paired-end sequencing libraries were prepared from QC-passed genomic DNA samples using the Illumina TruSeq Nano DNA Library Prep Kit. Approximately 200 ng of DNA was fragmented to a mean size of 350bp using the Covaris M220. End-repair and adapter ligation were performed to ensure low chimera formation, followed by size selection using AMPureXP beads. Indexed adapters were ligated for hybridization onto a flow cell.