thrid generation 16S rRNA seq of human saliva: participant (ECC)
After extracting total DNA, specific primers with Barcodes are synthesized for PCR amplification. The PCR products are purified, quantified, and normalized to create the sequencing library (SMRT Bell). This library undergoes quality control, and if it passes, it is sequenced using the PacBio Sequel system. The output BAM files are processed with SMRT Link software to generate CCS files. Data for different samples are identified by Barcodes and converted to FASTQ format.