RNA-Seq of mus musculus: adult female hippocampus, Wildtype
Mice were killed by cervical dislocation, their brains removed with chilled dissection instruments and the hippocampus dissected on ice. Tissue was immediately immersed in ice-cold RNAlater (Invitrogen, ThermoFisher) and stored at -20C. Tissue was homogenized in QIAzol lysis reagent using a TissueLyser LT bead beater (QIAGEN), and RNA was extracted using a QIAGEN RNeasy lipid tissue kit (Cat #74804) with on-column DNase I (Cat #79254) treatment following the manufacturers instructions. RNA quality was assessed with RNA ScreenTape (Agilent, Cat #5067-5576, 5067-5577 and 5067-5578) using an Agilent 4200 TapeStation. Samples with an RNA integrity number (RIN) > 7 were sent to the Australian Genome Research Facility for bulk RNA sequencing. Libraries were generated with the Illumina Stranded mRNA workflow with polyA capture, and samples sequenced to a depth of 20M reads with 150 base pair end lengths.