PE150 WGS of Mus musculus: adult female bone marrow
After Quant-iT PicoGreen (Invitrogen) quantification and quality control by Agilent TapeStation, 500 ng gDNA was sheared using a LE220-plus Focused-ultrasonicator (Covaris) and a sequencing library was prepared using the KAPA HyperPrep Kit (Roche) with modifications. Briefly, the library was subjected to a 0.5 size selection using AMPure XP beads (Beckman Coulter) after post-ligation cleanup. The library was not amplified by PCR and was pooled equivolume and quantitated based on its initial sequencing performance. The sample was run on a NovaSeq X in a PE150 run using the NovaSeq X 10B Reagent Kit (Illumina). The sample was sequenced for 1.3 billion reads, corresponding to 104 coverage.