10X single-cell TCR sequencing of P12_PBMC_C12D1-EOT
Sorted AIM-positive cells (i.e. cells positive for IFNγ and/or CD40L) for 24mer- and Pool-stimulated conditions were combined with 4000 AIM- unstimulated carrier cells. Combined cell populations were loaded into a single 10x Chromium NextGEM Single Cell 5' V(D)J v2 with Feature Barcoding reaction (10x Genomics 1000265, 1000190, 1000541, 1000252, 1000286, 1000215, 1000250). GEX, TCR, and surface feature (cell hashing) libraries were prepared according to the manufacturer's protocol. Libraries were sequenced on Illumina NovaSeq at read length 2x100bp for GEX libraries and 2x100-150bp for TCR and surface feature libraries.