scRNA-seq of Homo sapiens :female lung
Single-cell capturing and library construction were performed using the Chromium Next GEM Single Cell Reagent 3' Kits v3.1 (10x Genomics) according to the manufacturers instructions. The cell suspension, barcoded gel beads and partitioning oil were loaded onto the 10x Genomics Chromium Chip to generate single-cell Gel Beads-in-Emulsion (GEMs). Captured cells were lysed and the transcripts were barcoded through reverse transcription inside individual GEMs. Then cDNA along with cell barcodes were PCR-amplified. The scRNA-seq libraries were constructed by using the 3' Library Kits (PN-1000268, PN-1000215). The constructed libraries were sequenced on an Illumina NovaSeq 6000 platform to generate 2 150-bp paired-end reads.