Single-cell gene expression sequencing of peptide stimulated T cells from PBMCs
PBMCs from patient SJ1 were stimulated with DNAJB1-PRKACA fusion peptide EIFDRYGEEV (1 uM); half of cells were left unstimulated as a control. After stimulation, cells were stained with human hashtag/barcoded antibodies to distinguish between stimulated and unstimulated conditions. Live, CD11b-, CD19-, CD8+ cells cells were isolated by FACS and subjected to scRNAseq using the 10x Chromium platform using the Single Cell 5 V(D)J v1 with Feature Barcoding kit. Antibody barcode identities include HHash1_TotalC0251 with sequence GTCAACTCTTTAGCG, HHash2_TotalC0252 with sequence TGATGGCCTATTGGG