RNA sequencing results for sample 146_b
Nucleic acids were extracted from frozen tissues using the AllPrep DNA/RNA Mini Kit (QIAGEN catalog # 80204) according to the manufacturer's instructions. After RiboGreen quantification and quality control by Agilent BioAnalyzer, 107–500ng of total RNA with RIN values of 5.4–10 underwent polyA selection and TruSeq library preparation according to instructions provided by Illumina (TruSeq Stranded mRNA LT Kit, catalog # RS-122-2102). Samples were barcoded and run on a HiSeq 4000 in a PE100 run, using the HiSeq 3000/4000 SBS Kit (Illumina).