DNA seq of mouse: stool basal UC
Genomic DNA was extracted from fecal samples using the Zymo Research Fecal/Soil Microbe DNA Miniprep Kit, following the manufacturers protocol. DNA quality and concentration were assessed using a NanoDrop spectrophotometer and gel electrophoresis. The 16S rRNA gene libraries were prepared using the Illumina protocol, targeting the V3V4 hypervariable regions. Sequencing was performed on the Illumina MiSeq platform using a paired-end 2300 bp configuration. Library preparation and sequencing were carried out according to the standard Illumina recommendations.