RNA-Seq of SARS-CoV-2: wastewater
Wastewater samples were collected using a 24-hour flow-dependent composite sampler, and RNA was extracted from the concentrated primary samples. The presence of the SARS-CoV-2 virus was confirmed using digital droplet PCR. Whole-genome sequencing libraries of the SARS-CoV-2 were prepared according to the Illumina COVIDSeq Test protocol.