Pathogen: environmental/food/other sample from Clostridium botulinum
Genomic DNA was extracted with Wizard Genomic DNA Purification Kit (Promega, Fitchburg, WI, USA) and sequenced using the Next Generation Sequencing platform HiSeq 2000 (Illumina, Foster City, CA, USA) at the Institute for Molecular Medicine FIMM Technology Centre (University of Helsinki, Finland). Reads were mapped to the reference toxin gene cluster sequence using CLC Genomics Workbench (version 11.0.1; Qiagen, Hilden, Germany).