Nanopore DNA Sequencing of Human Gammaherpesvirus 4 strain Namalwa
An initial 12.5 ng of input DNA is amplified with sWGA and then debranching. 600 ng of the debranched product is distributed for whole genome multiplex PCR (200 ng), LMP2 singleplex PCR (300 ng), and EBNA2 singleplex PCR (100ng), which is followed by a second round of debranching. The final post-PCR products are consolidated for ONT sequencing, raw read preprocessing, and genome assembly by Canu and metaFlye. The two assemblies are then merged into a single assembly.