snRNA-Seq of mouse brainstem: Control1
C57BL6/J male mice were weaned at PND21 and exposed to CIH or maintained at room air from PND22 to PND43. The CIH group was subjected to intermittent episodes of hypoxia. Firstly, nitrogen (N2) was continuously injected into the chamber for 60 seconds, reducing the percentage of inspired oxygen (O2) within the chamber to a range of 4.5 to 5%. Subsequently, compressed air was injected for up to 5 minutes to restore the percentage of O2 to 21% before the initiation of a new hypoxia cycle. This protocol was repeated 80 times per day over 8 hours for 21 days. Mice were euthanized and the brainstem was frozen. Single nuclei suspension were prepared for each of two groups: CIH and Normoxia. The Chromium Next GEM Single Cell 3 Kit v3.1 (10x Genomics, Pleasanton, CA) was used to encapsulate single nuclei, cDNA preparation, and library construction according to the manufacturers protocol.