16S rDNA of mus musculus:tongue
The total DNA of tongue tissues was extracted and the bacterial DNA was amplified by PCR using primers targeting the V3-V4 region of bacterial 16S rDNA gene. The amplified products were collected by gel slicing method and quantified by QubitDNABR analysis kit is conjunction with the LifeQubit4.0 fluorescence spectrophotometer. The sequencing connectors were connected to construct the sequencing library, and the purified amplification products were mixed in the same amount. The denatured and indexed library had 10% denatured PhiX library (Illumina, San Diego, CA, USA) added to it. The denatured and indexed library, along with the added PhiX library, was loaded into the NovaSeq 6000 Reagent kit. The amplified fragments of the 16S rDNA gene were then sequenced on the Illumina NovaSeq 6000 platform.