Y-J7-15
Libraries were prepared from 200 ng of total RNA using the QIAseq stranded mRNA library kit (Qiagen), according to the manufacturers protocol. All libraries were controlled by Bioanalyzer 1200 (Agilent Technologies) and quantified by quantitative PCR (KAPA Library Quantification Kit for Illumina platforms, KapaBiosystems). Libraries were normalized and pooled in an equimolar way, before sequencing on a NovaSeq sequencer (Illumina) in 2  150 bp mode.