RNA-seq of normal nitrate treatment of CK arabidopsis biological duplication3
were performed by Novogene Corporation and sequenced on the Illumina HiSeq 2500 platform (Illumina, CA, USA). Clean reads were obtained by removing reads containing adaptors, reads containing ploy-N (> 10%) and low quality reads (Qphred  20) from raw data. Differential Expressed Genes (DEG) analysis were screened with the following criteria: |log2FoldChange|  1 and a false discovery rate (FDR) to adjust the P-values < 0.05