SQK-LSK109 library of Amycolatopsis bartoniae DSM 45807: R9.4.1 flowcell on GridION, guppy v6.1.2 with model dna_r9.4.1_450bps_sup
The library was prepared using the SQK-LSK109 ligation sequencing kit (Oxford Nanopore Technologies [ONT]) with native barcoding and sequenced on a GridION platform using a R9.4.1 flow cell. Base calling and demultiplexing were performed using guppy  v6.1.2 with model dna_r9.4.1_450bps_sup. Reads were trimmed with cutadapt -e 0.2 --trimmed-only -g AAGGTTAANNNNNNNNNNNNNNNNNNNNNNNNCAGCACCT followd by cutadapt -e 0.2 -a AGGTGCTGNNNNNNNNNNNNNNNNNNNNNNNNTTAACCTTAGCAAT -m 1000