Amplicon sequencing analysis by targeting the V4 region of the 16S rRNA gene for prokaryotes (amplified with 515f - 806r primer pair). A two-step amplification protocol was implemented, to construct amplicon libraries. A unique 12-bp index was added to the forward primer that served as a barcode to identify sequencing products in multiplexed libraries. 2x300 bp pair-end sequencing via the MiSeq platform (Illumina, San Diego, USA) was performed at at the Molecular Research Laboratory (MR DNA, Shallowater, TX, USA)
Amplicon sequencing analysis by targeting the V4 region of the 16S rRNA gene for prokaryotes (amplified with 515f - 806r primer pair). A two-step amplification protocol was implemented, to construct amplicon libraries. A unique 12-bp index was added to the forward primer that served as a barcode to identify sequencing products in multiplexed libraries. 2x300 bp pair-end sequencing via the MiSeq platform (Illumina, San Diego, USA) was performed at at the Molecular Research Laboratory (MR DNA, Shallowater, TX, USA)