DNA-Seq of Akkermansia: female infant fece
Library preparation was performed using NEBNextUltraTM  DNA Library Prep Kit for Illumina (NEB, USA, Catalog #: E7370L) with 0.2 g of total DNA per sample. Genomic DNA samples were fragmented to 350 bp by sonication, and the DNA fragments were end-polished, A-joint ligated and ligated to full-length adapters before further PCR amplification. Agencourt AMPure XP (Beverly, USA) purified the PCR productions. Sequencing was performed on the Illumina Miseq PE300 (Novogene, China, Peking) platform after the evaluation of library construction.