Whole genome sequence of Bacillus thuringiensis strain T121
Genomic DNA was extracted from Bt isolate T121 grown overnight at 30 degrees Celsius in LB broth following the protocol of Kalman et al. (1993). The concentration and purity of DNA were determined using Nanodrop Spectrophotometer (Genova, USA). The sequencing DNA libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit (Illumina, San Diego, USA). The whole genome of Bt isolate T121 was sequenced using llumina NovoSeq 6000 platform to produce paired end reads with read length of 150 bp