Control sample 4
Total RNA was extracted from the lysate of 3D stomach organoids using the Qiagen RNeasy Plus Universal Mini Kit. Libraries were constructed using the VAHTS Universal V8 RNA-seq Library Prep Kit for Illumina (Vazyme Biotech) and size-selected with VAHTS DNA Clean Beads (Vazyme Biotech) according to the manufacturers instructions. The libraries were sequenced by Illumina NovaSeq 6000 using a paired-end (150 bp for each end of the fragment) configuration.