Hi-C Chromatin Conformation Capture Sequencing of Tetraploid Wheat Kronos
Three proximity ligation libraries were prepared using 500 mg of juvenile leaf and immature inflorescence tissue from a single sibling plant used for PacBio library preparation. Library construction followed the manufacturers protocol for the Proximo Hi-C Plant Kit. Digestion was performed using the restriction enzymes DpnII (GATC), DdeI (CTNAG), HinfI (GANTC), and MseI (TTAA). Each library was sequenced as 150 bp paired-end reads on a single NovaSeq lane through Phase Genomics.