No_7
A transcriptome assay was performed on wild-type 'Nipponbare' and their T3 GS3-edited lines to identify downstream genes of GS3 involved in regulating grain size, grain quality, and thermotolerance. Total RNA was extracted from young panicles (2-4 cm) at the booting stage of both wild-type and GS3-edited lines. For each line, RNA was extracted from nine young panicles using the RNeasy Plant Mini Kit (QIAGEN, Darm-stadt, Germany), and subsequently pooled. The resulting libraries were sequenced using the Illumina HiSeq 2000 platform at Novogene Co., Ltd. (Beijing, China).