Resistant to Temephos
To investigate the midgut transcriptome of Ae. aegypti from RecR and RecL strains, 60 at 4th instar from each strain were used. The midguts of these larvae were dissected, separated into three pools of 20 each and stored in RNAlater until extraction. Total intestinal RNA was extracted using the RNeasy kit (Qiagen). Subsequently, the extracted RNA was submitted to an agarose gel electrophoresis to evaluate the quality of the samples. Total RNA contaminants presence and concentration were also determined by spectrophotometry using the NanoDrop 2000 spectrophotometer (Thermo Scientific) and the Qubit  2000 (Thermo Scientific), respectively. The total RNA input used in the following steps was 2 g of each  pool of samples