RNA-seq of peanut leaves under well watered condition
Total RNA was isolated from peanut leaves with Trizol reagent (Ambion, USA), treated with DNase TURBO DNA-free Kit (Ambion, USA) for DNA digestion and quantified with Qubit 2.0 fluorometer (Life Technologies). The mRNA purification was performed utilizing Dynabeads mRNA Purification Kit (Ambion, USA). Purified mRNA (100 ng) was used to construct whole transcriptome library using Ion Total RNA- Seq Kit v2 Reaction kit (Thermo Fisher Scientific). Each fragment of cDNA was clonally amplified by emulsion PCR using the Ion PI Hi-Q OT2 200 Kit (Thermo Fisher Scientific).  The libraries were sequenced using the Ion PI Sequencing 200 Kit v2 on Ion Proton sequencer (Thermo Fisher Scientific). All the protocols were followed according to user manual.