Pacbio genome sequencing of Abudefduf troschelii
High molecular weight DNA was extracted from frozen muscle tissue of one individual of A. troschelii using a phenol:chloroform method. We additionally cleaned this DNA up with 3X KAPA pure Beads (Roche Sequencing) and concentration and purity of the extracted DNA was assessed using a Qubit fluorometer (Thermo Fisher Scientific). We also checked for the integrity of the DNA with a field inversion gel (Pippin Pulse, Sage Science). On average, DNA fragments were 50 kb in length. We prepared a PacBio HiFi library with the SMRTbell Express Template Prep Kit 2.0 (PacBio 100-938-900) using the PacBio low input protocol (DNA sheared to 15 kb) for HiFi sequencing followed by AMPure bead cleanup. The genomic library was sequenced on a single 30-h movie 8M SMRT cells in CCS mode on the PacBio Sequel II system at the Brigham Young University (BYU) DNA Sequencing Center.