16S rRNA gene Amplicon-Seq of bacterial microbial community in Chromochloris zofingiensis culture: 338F(5'- ACTCCTACGGGAGGCAGCAG -3')-806R(5'- GGACTACHVGGGTWTCTAAT -3')
Total microbial genomic DNA was extracted from samples using the E.Z.N.A. soil DNA Kit, qualitychecked by 1% agarose gel and NanoDrop2000, the V3V4 region of 16S rRNA was PCRamplified with primers 338F/806R using Fast Pfu polymerase (95  3 min; 27[95  30 s, 55  30 s, 72  45 s]; 72  10 min), purified from 2% agarose gel, quantified by Qubit 4.0, pooled equimolarly, and pairedend sequenced on an Illumina Nextseq2000.