Cp genome of Hopea rudiformis
Sequencing libraries were constructed using the xGen DNA Library Prep EZ UNI kit (Integrated DNA Technologies, IA, USA). Enzymatic fragmentation of gDNA yielded inserts averaging 300 bp in size. Libraries were quantified and validated for size distribution using the Agilent 4150 TapeStation. Paired-end sequencing (150 bp read length) was performed on an Illumina NextSeq 2000 platform (Illumina, CA, USA) at Genetika Science Lab (Tangerang, Indonesia), generating approximately 5 Gb of raw sequencing data to ensure sufficient coverage for chloroplast genome assembly.