HS skin from donor_2
Skin (3x3 cm) was placed in 5 ml of medium 154 supplemented with human keratinocyte growth supplement and cut into small pieces. Freshly prepared 100l Liberase TL (2mg/mL stock, Roche Diagnostics) + 700l of Tyrodes solution was added to the tissues and incubated for 15 min at 37C and tissue was dispersed through a 70m cell strainer (Falcon). Cells were then re-suspended in 300-500 l of sterile PBS with 0.04% BSA  and centrifuged at 2000 rpm for 5 minutes. Cell viability was checked using 7-AAD Cells. Cells were processed for SingleCell RNA sequence. Single cells were loaded into 10xcontroller and individual cells were be captured into oil droplets to form Gel Bead-In-EMulsions (GEMs). The single cell cDNA libraries were constructed according to manufacturers protocol provided by the Chromium NextGEM single cell 3 reagent kit (10XGenomics, version 3.1).