16S rRNA of bacteria: silage
To isolate genetic material from silage, the Stool DNA Purification Kit (EURx, Poland) was used and homogenized in a TissueLyser LT homogenizer (Qiagen, Germany) for 10 min, 50 rpm per min. Total DNA isolation was then performed according to the instructions provided by the kit manufacturer. Elution was performed in 100 l of the elution solution, followed by DNA quality analysis (Nanodrop 2000c, Thermo Fisher Scientific, Lithuania) and fluorimetric DNA quantity measurement (Quantus, Promega, Germany). Then, the default instructions for the 16S Barcoding Kit 24 V14 (Oxford Nanopore Technologies, United Kingdom) were followed (https://nanoporetech.com/document/rapid-sequencing-DNA-16s-barcoding-kit-v14-sqk-16114-24)