WGS of phage SS01
Assembly was attempted with multiple approach (Flye meta mode, haploflow and Flye normal mode), out of which best results (longer contig and lesser no. of contigs) were obtained from Flye normal mode option. Assembly was attempted with multiple approach (Flye meta mode, haploflow and Flye normal mode), out of which best results (longer contig and lesser no. of contigs) were obtained from Flye normal mode option. The assembly resulted in contig with maximum length around 46.7 KB and blast homology search against NCBI NR/NT database identified the sequence having maximum homology (~91% identity, 95% coverage) with Staphylococcus phage TPWB-09. As per PRS, expected genome size is ~150 KB but assembly result reports around 47 KB. Sequencing was performed on a MinION/GridION/PromethION platform to generate long-read data for de novo assembly and annotation of the phage genome.