Microbe sample from Variovorax sp. SPNA7
DNA was extracted using the Zymo D6060 Quick-DNA HMW Magbead Kit per the manufacturers instructions (https://www.zymoresearch.com/products/quick-dna-hmw-magbead-kit). DNA was fragmented using using Covaris gTubes following instructions from the manufacturer (4 passes at 7000 rpm through the gTube orifice). Average size of the sheared gDNA was checked at the TapeStation 4200 (Agilent).  Multiplexed Microbial libraries were prepared using the Pacbio SMRTbell prep kit 3.0 together with the SMRTbell barcoded adapters 3.0 according to the PacBio protocol.  Final Whole genome libraries were not size selected but simply purified by standard clean up with 1X SMRTbell clean up beads. DNA sequencing was performed using the PacBio Sequel IIe platform.