WGS of Mus musculus Mgme1 KO
WGS library construction and sequencing were performed at the Center for Genome Technology at the John P. Hussman Institute for Human Genomics, University of Miami Miller School of Medicine. Sequencing libraries were prepared with the TruSeq DNA PCR-free HT sample preparation kit from Illumina. In brief, 1 ug of total genomic DNA was fragmented, using the Covaris LE220 focus acoustic sonicator to a target size of 350 bp. Blunt-end DNA fragments were generated, and size selection was performed with AMPure bead purification (Beckman Coulter). A-base tailing was performed on the 3 blunt ends, followed by adapter ligation and a bead-based cleanup of the libraries. Final library fragment size was evaluated on the TapeStation (Agilent Technologies) and final molarity quantification was determined by quantitative polymerase chain reaction with adapter-specific primers (Kapa Biosystems) on a Roche Light Cycler. Samples were pooled for sequencing on a 10B flow cell on the NovaSeq X Plus. FASTQ files were generated with the Illumina DRAGEN FASTQ generator software.