RNAseq HLF 0.01%DMSO treatment
Briefly, 500 ng of total RNA was used as input material for library preparation, performed with the Illumina TruSeq Stranded Total RNA Kit (Illumina, Cambridge, UK), according to manufacturers instructions. Dual Indexes were made using the Illumina Truseq RNA UD indexing plate to ensure accurate library sequencing.  The resulting libraries were sequenced on the Illumina NextSeq 500 (Illumina) to generate 75 base-pair single-ended reads.