Single Nucleus RNA-seq of mus musculus: colon tissue
Single-nucleus RNA sequencing (snRNA-seq) was performed on mouse colon using the 10X Genomics Chromium Single Cell 3' v3.1 platform. Nuclei were isolated via NP-40 lysis, DAPI-stained, and sorted by FACS. Libraries were generated via barcoded reverse transcription and cDNA amplification, followed by standard library preparation and sequencing on an Illumina NovaSeq 6000.