Investigate the off-target of the CRISPR-CasRX system in the silkworm
For RNA sequencing, total RNA was extracted from BmE cell line by Trizol (Invitrogen, USA), then grinded with liquid nitrogen. RNA was extracted again by trichloro methane, precipitated by isopropanol and cleared by 75% ethanol, RNA samples were stored in a refrigerator at 80C for sequencing libraries. Each cDNA sample was clustered using Oligo-dT magnetic beads and then sequenced on the HiSeq2504 (Illumina, USA) platform using Single-read technology.