WGS with Omni-C (Dovetail) of Epinephelus coioides liver
DNA from fresh frozen Epinephelus coioides liver extracted with with the Omni-C kit (Dovetail); after reversal crosslinking, DNA was purified and prepared for Illumina-compatible paired-end sequencing libraries; biotinylated chimeric molecules were isolated using streptavidin beads before PCR enrichment of the library through amplification with 12 PCR cycles using KAPA HiFi HotStart Ready Mix (Roche); library sequenced on Illumina NovaSeq 6000 with a read length of 2x151bp.