PCR amplicon of nosZ gene of Platismatia glauca
The RNA of Platismatia glauca was isolated which was reverse transciptased to cDNA, on which PCR product of nosZ was produced and purified. The 1st PCR with sequencing adaptors were added on purified PCR product. The sequencing service lab added the indexes in 2nd PCR and did the Illumina MiSeq sequencing.