Complete Genome Sequence of Avian Influenza A (H5N1) Virus Isolated from Chicken During a Recent Outbreak in Bangladesh
Specimen collection, Viral RNA extraction using QIAamp Viral RNA Mini Kit (Qiagen, Hilden, Germany), RT-PCR detection, isolation by chicken embryonated egg, Viral RNA extraction from allantoic fluid, confirmed by RT-PCR, cDNA preparation and multiplex ARTIC PCR to amplify viral genome sequences, Pooling and purification of ARTIC PCR products, Quantification of pooled and purified ARTIC PCR products, Preparation of DNA ends for barcoding, DNA library sample barcoding and adapter ligation, Loading and running the Oxford Nanopore MinION MK 1C platform, Bioinformatics workflow for the MinION pipeline.