. IITD104
The high molecular weight DNA was enzymatically fragmented and end repair was done to produce blunt ends. The blunt ended fragments were adenylated, loop adapters were ligated and then cleaved with uracil-specific excision reagent (USER) enzyme. AMPure beads were used to further purify the samples, and the DNA was amplified by 6 cycles of PCR using NEBNext Ultra II Q5 master mix, universal illumina primer, and sample-specific octamer primers.