RNA-seq of the murine adipocyte cell line 3T3-L1 following lentiviral overexpression of bottlenose dolphin ADRB3
Total RNA was extracted from 3T3-L1 cells using TRIzol (Vazyme). Library preparation of 1 g of total RNA, using a TruSeq RNA Sample Preparation Kit v2 (Illumina, San Diego, CA), and 150-bp paired sequencing on the Illumina HiSeq X Ten platform was performed by Novogene (Beijing, China).