genome-seq of G7: Raphidiopsis raciborskii
Fragment genomic DNA using a 26G needle, select fragments larger than 20kb using BluePippin, perform end repair and A-tailing, then ligate adapters to both ends of the fragments to prepare the DNA library. After the library passes quality control, perform sequencing using the PacBio Sequel IIe platform based on the effective concentration of the library and data output requirements.