DNA Seq of Environmental Metagenome: Litter
At seven days of age, during the first grow out, the birds were orally gavage with a solution containing 1.0x10 7 colony forming units (CFU) of a nalidixic acid-resistant Salmonella Enteritidis strain. Water treatments were administered continuously through the drinker lines connected to water reservoir buckets. They consisted of a first group with acetic acid [CH3COOH] and lactic acid [C3H6O3] (organic acid blend), a second group with citric acid [C6H8O7] and a third group with untreated water. Each group with 24 repetitions (pens). After the first grow out, litter treatments included windrow composting and Aluminum sulfate (Al+Clear). Litter samples were taken after every grow out and after the litter treatment. DNA was extracted from litter samples and the 16S rRNA V4/5 region was amplified by PCR. The products were sent for Illumina sequencing.