RNA-Seq of Inner Flesh:  9 Week Post-Pollination Kiwifruit
Total RNA was extracted from kiwifruit samples, followed by mRNA enrichment or rRNA depletion. The mRNA was fragmented and reverse-transcribed using random N8 primers. The cDNA underwent second-strand synthesis, followed by ER/A-tailing and adapter ligation. The library was then amplified through PCR and heat-separated. Finally, single-stranded circular DNA (ssCirc) was formed, followed by DNB (DNA nanoball) formation and sequencing.