Root RNA-seq: Control group
Library preparation was performed using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina Inc., San Diego, CA, USA), involving the random fragmentation of cDNA generated from the mRNA sample, followed by the ligation of 5' and 3' adapters. Prior to sequencing, clusters were generated by loading the library onto a flow cell, where fragments were captured on a surface-bound lawn of oligos complementary to the library adapters. Each fragment was then amplified into distinct clonal clusters through bridge amplification. Sequencing was subsequently performed on paired-end reads of 150 base pairs using an Illumina HiSeq 2500 platform