Single-cell RNA-seq of Mus musculus: Mock-Infected Small Intestine
RNA in individual cells of the fixed samples was sequenced using Single Cell Gene Expression Flex (10X Genomics, USA) according to manufacturers instructions. Briefly, fixed samples were thawed on ice and mixed with mouse probes and hybridized overnight (18 hours) at 42. The samples then were washed and counted. Samples were loaded into the Next GEM chip Q and run on Chromium X. GEMs were recovered and pre-amplified by PCR reaction. Library was constructed and sequenced on DNBSEQ-G400RS.