18S-rRNA-Seq of arbuscular mycorrhizal fungi in Xihe Sb mine soil
Amplicons were extracted from 2% agarose gels and purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, U.S.) according to the manufacturers instructions and quantified using QuantiFluor-ST (Promega, U.S.). Purified amplicons were pooled in equimolar and paired-end sequenced (2251) on an Illumina MiSeq platform (Majorbio, Shanghai, China) according to the standard protocols.