Metagenomic Insights into Anaerobic Toluene Biodegradation by a Sulfate-Reducing Microbial Community
Barcoded SQK-NBD114.96 DNA libraries were prepared with minor modifications to the manufacturers protocol (Oxford Nanopore Technologies, United Kingdom). The libraries were loaded onto primed FLO-PRO114M (R10.4.1) flow cells and sequenced on a PromethION P24 device running MinKNOW Release 24.02.19. Signal data was basecalled and demultiplexed using Dorado basecall server v. 7.3.11 (Oxford Nanopore Technologies) with the super-accurate (SUP) algorithm (dna_r10.4.1_e8.2_400bps_5khz_sup.cfg).