RNA-seq of Pseudomonas aeruginosa
Briefly, the total RNA was extracted using the SV Total RNA Isolation System (Promega, USA) , and the RNA quality and quantity were checked using Bioanalyzer (Agilent, USA) and RNA 6000 Nano kit (Anilent, USA). Then, the Ribo-Zero rRNA removal kit  was used to remove rRNA, the cDNA libraries was constructed and sequenced on an Illumina HiSeq 2500 sequencer (Illumina, USA). The Reads Per Kilobase Per Million Read (RPKM) and the false discovery rate (FDR) (q value) was measuered to determine the gene expression changes.