Single-cell gene expression sequencing of TILs obtained directly from FLC
T cell present in dissociated tumor from patient SJ4 were stimulated with 24mer DNAJB1-PRKACA fusion peptide (RKREIFDRYGEE-VKEFLAKAKEDF, 1 uM). After stimulation, live cells were isolated by FACS and subjected to scRNAseq using the 10x Chromium platform using the Single Cell 5 V(D)J v1 kit.