RNA-seq of Apis mellifera (IL): DWV_B/A recombinant
Viral RNA was extracted from 200L of homogenized whole-body bees using the NucleoMag Virus kit and KingFisher Flex. cDNA synthesis was performed using Template Switching RT Enzyme with random N6 primers, followed by second-strand synthesis with PrimeSTAR HS Polymerase. The resulting cDNA was purified using CleanNGS beads and quantified using Qubit. Sequencing libraries were prepared with the Native Barcoding Kit and sequenced on Oxford Nanopore's GridION platform using R10 flow cells for 72 hours.