Gravel biofilm from NISA STP
12.5 ng DNA from sample was subjected to 16S V3-V4 sequencing using primers. The PCR products were bead purified and subjected to another round of PCR with dual indices and adapters to generate the respective libraries as recommended by illumina. The libraries were quantified on Qubit 3 fluorometer using DNA HS assay kit and appropriate dilutions loaded on a The Tapestation 2200 using High Sensitivity D1000 ScreenTape assay, to determine the size range of the fragments and the average library size.