Subsample of Basque Lake: Control_neg1 Forward read
Genomic DNA libraries were prepared using the Nextera XT Library Prep Kit (Illumina, San Diego, USA) and sequenced on an lllumina NovaSeq 6000 (Illumina, San Diego, USA) using a 250 bp paired end (Bankevich et al., 2012; Bolger et al., 2014).  Coverage of 53-fold was achieved, calculated using BWA, SAMtools (v0.1.19), and BEDTools genomecov (v2.2.7) with default settings (Li et al., 2009; Li and Durbin, 2009; Quinlan and Hall, 2010).