Genome sequencing of Mucor janssenii CBS 205.68
One microgram of genomic DNA was subjected to library preparation using the SQK-LSK109 and EXP-NBD104 to generate a multiplex library. The prepared library was loaded onto a FLO-MIN106D flow cell and data was collected using MinKNOW. Raw data was rebasecalled with MinKNOW v24.11.8 using the build-in dorado basecaller in super accuracy basecalling with model basecall_model_version_id=dna_r9.4.1_e8_sup@v3.3. All data was collected into a single FASTQ file and subjected to chopper to select for fragments >1100bp and with a Q of 10 and higher, thereafter selected reads were trimmed (50bp head and tail) and collected in the final FASTQ for further downstream analyses.