Ultra-long read sequencing of COLO 320DM with Oxford Nanopore
High-molecular weight (HMW) genomic DNA was extracted from approximately 6 million COLO320-DM cells using the Monarch HMW DNA Extraction Kit for Tissue (NEB #T3060L) following the Oxford Nanopore Ultra-Long DNA Sequencing Kit V14 protocol. After extracting HMW gDNA, we constructed Nanopore libraries using the Oxford Nanopore Ultra-Long DNA Sequencing Kit V14 (SQK-ULK114) kit according to manufacturers instructions. We sequenced libraries on an Oxford Nanopore PromethION using a 10.4.1. Flow Cell (FLO-PRO114M) according to manufacturers instructions. Basecalls from raw POD5 files were computed using Dorado (v.0.2.4).