RNA-Seq of A2780
Total RNA was extracted from A2780 cells using the TRIzol reagent kit (Invitrogen; Carlsbad, CA, USA) according to the manufacturers protocol. The purity and concentration of the extracted RNA were evaluated by the NanoDrop 2000 optical test. RNA quality was assessed on an Agilent 2100 Bioanalyzer (Agilent Technologies; Palo Alto, CA, USA) and checked by RNase-free agarose gel electrophoresis. All samples were qualified.