P1031_iPSC_C4P20_UDID181_ChIPCTCF_HH_20161011_R01L01S01
Detailed methods are found in PMID 28388430. Cells were cross linked with 1% formaldehyde for 10min at room temperature. For each sample 3x10^6 cells were lysed and sonicated using Covaris M220 for 10min. Sonicated chromatin were immunoprecipitated with anti-CTCF antibody (Santa Cruz Biotechnology, sc-15914 X). Libraries were prepared according the method described in Panopoulos et al., (2017) and Yan et al., (2013) using Illumina TruSeq adapters, size selected for 300bp-500bp, and sequenced on Illumina HiSeq4000 for paired-end sequencing to obtain 100bp reads.