libNP1900_31567bp_Excision_Site1_TBL-Site2_DBL_EnhSplit_FigS14_Rep3
gDNA is extracted from edited cells using QuickExtract DNA Extraction solution (Biosearch Technologies). DNA is randomly tagmented with Tn5 to introduce adapters. Nested PCR is performed to amplify the junction from one excision/inversion substrate and the downstream sequence, capturing both rearranged and non-rearranged junctions.