Mature red cedar transition zone
There are two ways to obtain mRNA: one is to enrich mRNAs with polyA tails by Oligo(dT) beads, taking advantage of the fact that most of the eukaryotic mRNAs have polyA tails; the other is to obtain mRNAs by removing ribosomal RNAs from the total RNA. mRNAs are then randomly interrupted in Fragmentation Buffer by divalent cations (see below). The mRNA was then randomly interrupted with divalent cations in the Fragmentation Buffer, and the libraries were constructed according to the common library construction method or strand-specific library construction method.