Pooled fecal sample from stress-exposed donor mice - 2
DNA was extracted from pooled fecal samples using the QIAmp Power Fecal Pro DNA Kit (QIAGEN). The V4 region of the 16S rRNA gene was amplified using primers 515F/806R and libraries were prepared with the Nextera XT DNA Library Prep Kit (Illumina). Sequencing was performed on an Illumina MiSeq platform using a v2 reagent kit (500 cycles, 2x250 bp paired-end reads).