Whole genome sequence of Rhodococcuss sp. IITD102
The intact DNA was enzymatically fragmented to undergo end repair for development of blunt ends. To the blunt ended fragments, adenylation was performed and loop adapters were ligated and then cleaved with uracil-specific excision reagent (USER) enzyme. AMPure beads were used to further purify the samples, and the DNA was enhanced by 6 cycles of PCR using NEBNext Ultra II Q5 master mix, universal illumina primer, and sample-specific octamer primers.