Transcriptome Sequencing of Asian Common Toad, Duttaphrynus melanostictus DM2
Total RNA was extracted from Duttaphrynus melanostictus specimens to capture a comprehensive representation of expressed transcripts. RNA quality and integrity were assessed prior to cDNA library preparation. Strand-specific libraries were constructed following standard protocols and sequenced on an Illumina high-throughput platform to generate paired-end reads. The design aimed to maximize transcriptome coverage for accurate de novo assembly, annotation, and gene discovery. This sequencing strategy facilitates the identification of coding and non-coding RNAs, supports functional and evolutionary studies, and provides a valuable resource for understanding the molecular biology and ecological adaptations of this amphibian species.