Fourth technical replicate of a 3RK_0_CG17360 amplicon library from the control pool 2 of flies used to assess assay accuracy and variance
Fourth technical replicate of a PCR-based amplicon library targeting 7609417-7609723 on chromosome 3R (D. mel. reference genome release 5), prepared for Illumina paired-end read sequencing in order to assay the frequency of In(3R)K. Pooled individuals were used for DNA extraction as described (lightly modified from Langley et al., 2011). Libraries were prepared following a lightly modified version of the Illumina metagenomic amplicon protocol (Illumina, 2013) using custom oligos ordered from IDT. Sequencing was conducted at the Next Generation Sequencing Core of University of Wisconsin Madison Biotechnology Center (https://dnaseq.biotech.wisc.edu), using the Illumina NovaSeq6000 to generate 150bp paired end reads to a depth of at least 20000.