RNA-Seq of mus musculus: bone marrow-derived, Ly6g mibrobead-isolated neutrophils
8-week-old female wild-type C57BL/6 mice were euthanised for collection of bone marrow cells, which underwent either ferrous ammonium citrate or sham treatment in vitro. Total RNA was then extracted from the Ly6g microbead-isolated cells using standard protocol. The mRNA was enriched, fragmented and reversibly transcribed into cDNA. The purified double-stranded cDNA was end-paired, sequence-added, ligated to the sequencing adapters and amplified for library construction before eventually sequenced. The standard bioinformatic workflow was then applied for analysis.