Transcriptome_C4
Total RNA was extracted from 500 uL of concentrated Colpoda sp. cultures, with and without IAA treatment, using the Quick-RNA Miniprep Kit (Zymo Research). RNA quality and quantity were assessed using the Qubit HS RNA assay (Invitrogen). Strand-specific paired-end libraries were prepared using the Zymo-Seq RiboFree Total RNA Library Kit (Zymo Research) following the manufacturer's protocol. Sequencing was performed on the Illumina NovaSeq platform, generating high-depth transcriptomic data for four biological replicates per condition.