RNA-Seq of Culicoides reevesi
Total RNA was extracted from homogenized insects using Trizol Reagent. Ribosomal RNA was depleted from the sample using the NEBNext rRNA Depletion Kit (New England BioLabs) and an RNAseq library was generated using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs). Unbiased high-throughput sequencing was performed at the Iowa State University DNA Facility.