Illumina RNAseq sequencing of Sporisorium scitamineum SSC04: 48 hours post-inoculation in sugarcane genotype IAC66-6
The experiment was conducted in three replicates, combining ten buds of inoculated plants and three replicates of ten buds for mock-inoculated plants. Forty-eight hours post-inoculation, buds were collected in liquid nitrogen for RNA extraction. Total RNA was extracted using the LiCl-based method. The RNAseq experiment used a depletion of rRNA from the fungus and the plant before library preparation and sequencing of total RNA. Libraries were prepared with Illumina Stranded mRNA Prep, Ligation kit, and RNA paired-end and sequenced on the Illumina NovaSeq 6000 platform at Center for Research Support Facilities, University of So Paulo (CEFAP), USP, So Paulo, BR to produce 101-bp paired-end reads.