Amplicon sequencing of Human immunodeficiency virus 1:SupT1_v100_c1000_r3
Total nucleic acid was purified using Qiagen DNA/RNA purification kit. The RNA was reverse transcribed using RT primer (CAAGTGCCTAGATCCTCGAGNNNNNNNNNNNNNNNNNNNNNCACTTGCCACCCATCTTATA) and purified by Invitrogen PCR clean-up kit. The barcode region was amplified using primers CAAGTGCCTAGATCCTCGAG and GGCTTGGAAAGGATTTTGCTATAA. The PCR product was confirmed using gel electrophoresis and purified by the PCR clean-up kit. I then used NEBNext Ultra II DNA library prep kit to make pair-end sequencing libraries.