hFRPE_D56_ATAC_R04L01S01
We performed ATAC-seq using the protocol from Buenrostro et al. (Buenrostro et al., 2013) with small modifications. Frozen nuclear pellets of 5 x 10^4 cells each were thawed on ice and tagmented in permeabilization buffer containing digitonin. Tagmentation was carried in 25ul using 2.5ul of Tn5 from Nextera DNA Library Preparation Kit (Illumina) for 60 min at 37C in a thermomixer (500 RPM shaking). To eliminate confounding effects due to index hopping, all libraries within a pool were indexed with unique i7 and i5 barcodes. Libraries were amplified for 12 cycles. Libraries were sequenced to approximately 80M 150bp paired end reads on the HiSeq 4000 (Illumina) platform.