Shotgun seq of whole blood from patients with suspected bloodstream infection
10 mL whole blood samples were pretreated with Molzym add-on 10 for initial human depletion followed by automatically extraction with SelectNA Blood Pathogen Kit (Molzym, Germany) on a NorDiag Arrow instrument (DiaSorin, Italy). DNA concentration (Qubit) and mean read length (TapeStation, Agilent, USA) was analysed and libraries were pooled together in final concentrations of 20 nmol/L (or maximum 20 L) per sample. Sequencing performed on Illumina MiSeq (600 cycles).