paddy fields cultivated on saline-alkali soils for 11 years at Daan plot 2
Soil DNA was extracted from 0.5 g soil from each sample using the FastDNA Spin Kit (MP Biomedicals, California, USA). The DNA concentration and purity were examined before being sent to Beijing Biomarker Technologies Co., Ltd., China, for shotgun metagenomics sequencing on the Illumina HiSeq 2000 platform with a paired-end approach.