RNA-Seq of Homo sapiens: adult male PBMC
Multiplex PCR reactions were run to specifically amplify the third complementarity-determining region (CDR3) of the TCR chain for library construction by KC-Digital Stranded TCR-seq Library Prep Kit. The constructed libraries were deeply sequenced on Illumina (SeqHealth, China). Raw sequences filtered by SOAPnuke (version 2.1.0) were used for TCR sequencing analyses, and the sequencing data were mapped to the ImMunoGeneTics (IMGT) database using MiXCR (version 3.0.3) to define the V, D, and J fragments and CDR3 sequence.