tongue of mouse
The total DNA of tongue tissues was extracted and the bacterial DNA                 was amplified by PCR using primers targeting the V3-V4 region of bacterial 16S rDNA                 gene. The amplified products were collected by gel slicing method and quantified by                 QubitDNABR analysis kit is conjunction with the LifeQubit4.0 fluorescence                 spectrophotometer. The sequencing connectors were connected to construct the                 sequencing library, and the purified amplification products were mixed in the same                 amount. The denatured and indexed library had 10% denatured PhiX library (Illumina,                 San Diego, CA, USA) added to it. The denatured and indexed library, along with the                 added PhiX library, was loaded into the NovaSeq 6000SP Reagent kit. The amplified                 fragments of the 16S rDNA gene were then sequenced on the Illumina NovaSeq 6000                 platform.