RNA-seq of Saccharomyces cerevisiae rox1 t60 repl3
Three independent yeast colonies were used for inoculation. Overnight cultures were diluted in fresh medium. Cells were grown for 3 hours, treated with -factor for 2 hours, washed once in YPAD medium and released in YPAD containing 0.2M HU. Samples were collected after -factor synchronization (t0) and after release of 60 minutes in HU (t60). Total RNA was isolated using the RNeasyMini kit (Qiagen) and treated with the RNase-Free DNase Set (Qiagen) to remove any contaminating genomic DNA. Three independent biological replicates for each condition were subjected to sequencing on Illumina NovaSeq6000 PE150.