Genomics Analysis Reveals Molecular Patterns of Tumorigenesis in HPV-associated and HPV-independent sinonasal squamous cell carcinoma/The goal of this study was to identify molecular mechanisms of tumorigenesis in HPV-associated and HPV-independent sinonasal carcinoma samples using high-throughput sequencing approaches.
Libraries for WES were constructed using the Agilent SureSelect V5 post-capture beginning with a minimum of 200ng of genomic DNA and the SureSelectXT Target Enrichment System for Illumina paired-end multiplexed sequencing. Paired-end sequencing was performed using the NovaSeq 6000 S4 Flow Cell with 2 × 150 cycle chemistry with an output of approximately ~8Gb (150X) per sample.