scRNA-seq of Oncorhynchus mykiss: white muscle of 1.5kg animals
scRNA-seq libraries were prepared from Muscle Derived Cells cells freshly isolated from trout muscles following the 10X Genomics Single Cell 3' V3.1 Gene Expression Reagent Kits. cDNA from the reverse transcription reaction was purified using DynaBeads MyOne Silane Beads. The libraries were then fragmented, end-repaired and A-tailed, amplified and indexed with dual sample indexes i5 and i7 through PCR amplification, then sequencing on a NovaSeq 6000