rrn operon amplicon library of sample F1_21
amplicons prepared with primers 16S-27F (5- TTTCTGTTGGTGCTGATATTGC AGrGTTTGATyhTGGCTCAG) and 23S-2241R (5-ACTTGCCTGTCGCTCTATCTTC ACCrCCCCAGThAAACT-3), indexed in the second PCR reaction with custom primers and further library prepared with Oxford Nanopore Sqk-lsk114 Ligation Kit, sequencing using R10.4.1 flow cell, basecalling using dna_r10.4.1_e8.2_400bps_sup@v4.3.0 model