RNA seq of normal human bronchial epithelia cells after 24 hours of colonization with Fusobacterium nucleatum ATCC 25586 rep. 2
Samples were prepared for whole genome sequencing using an Illumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus Microbiome and unique dual indexes. Sequencing was performed on the Illumina NextSeq2000 platform using a 300 cycle flow cell kit to produce 2x150bp paired reads. Read demultiplexing, read trimming, and run analytics were performed using DRAGEN v3.10.12, an on-board analysis software on the NextSeq2000. We include fastqc metrics as a best practice and for examination in the case of unexpected outputs.