Genomics Analysis Reveals Molecular Patterns of Tumorigenesis in HPV-associated and HPV-independent sinonasal squamous cell carcinoma/The goal of this study was to identify molecular mechanisms of tumorigenesis in HPV-associated and HPV-independent sinonasal carcinoma samples using high-throughput sequencing approaches.
Libraries for WGS were constructed using TruSeq Nano DNA Library Prep kit with a minimum of 100ng of genomic DNA per sample. Paired-end sequencing was performed using the NovaSeq 6000 S4 Flow Cell with 2 × 150 cycle chemistry with an output of approximately ~110Gb (30X) per sample.