BCL11A amplicon sequencing : CD33plus cells from NSG mice engrafted with human CD34+
Human mobilized CD34+ HSC were edited at the BCL11A enhancer using zinc-finger nucleases. Editing levels were assessed via PCR amplification of the enhancer followed by MiSeq sequencing. A 140bp amplicon design was used. Fastq files were filtered to remove potential contamination from other amplicons.