Single-cell transcriptomics of diverse free-living Cercozoan protists
A single cell was isolated via manual pipetting, photodocumented, and deposited in SmartSeq2 lysis buffer (Picelli et al 2014). Cells were lysed by several freeze-thaw cycles. cDNA was generated from cells following Picelli et al 2014, using a template-switching oligo (TSO) and subsequent PCR with 20-24 amplification cycles. After cleanup with magnetic beads this cDNA was then used as input into Illumina DNA Library Prep kit and sequenced on various instruments on the Illumina platform.