RNA-seq of Homo sapiens: adult colon cancer
RNA was quantified with NanoDrop and quality-checked with TapeStation. Libraries were prepared from 800 ng RNA using TruSeq Stranded Total RNA Library Prep Gold, quantified with TapeStation and Qubit, pooled at 1.5 nM, and sequenced on an Illumina NovaSeq 6000 (2100 bp, paired-end).