shortly ampicated DNA of mus musculus: crRNP-transfected CD6 T cells
crRNA and tracrRNA are denatured in a PCR machine at 95C for 5 minutes, followed by annealing of the crRNA-tracrRNA duplex through a 1-hour incubation at room temperature. Cas9 is then added to form the crRNP complex. CD6 T cells, isolated from the splee_x0000__x0000__xFFFF__xFFFF__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__xFFFF__xFFFF__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0010__x0000__x0000__x0008__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000_ _x0000__x0000__x0000__x0000__x0000__x0004__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0002__x0000__x0000__x0000__x0008__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0004__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0002__x0000__x0000__x0000__xFFFF__xFFFF__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0010__x0000__x0000__x0010__x0000_ _x0000__x0000__x0007__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0003__x0000__x0004__x0000__x0010__x0000__x0004__x0000__x0000__x0000__x0004__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000_ _x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0002__x0000__x0000__x0000__xFFFF__xFFFF__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000_P_x0000__x0000__x0000__x0000__x0000__x0000_