Population genomics of Onchocerca volvulus
Nodules containing nematodes were surgically extracted 90 days post-ivermectin treatment. Nodules were treated with collagenase and individual females removed. DNA was extracted from the posterior end of each worm (i.e., from non-reproductive tissue). Worms from Ghana were sequenced using a whole genome sequencing approach. Sequencing libraries were created from DNA extractions using the NEBNext Ultra library kit protocol (New England Biolabs) and 100-bp paired-end sequences were generated on an Illumina HiSeq at either the La Trobe University Genomics Platform or the McDonnell Genome Institute at Washington University.