Control group
From receiving the sample to obtaining the final sequencing data, which includes steps such as DNA extraction and QC, PCR amplification and QC, library construction and QC, and on-board sequencing. Initial quantification of library concentration was performed using Qubit 4.0. Qsep400 was used to check the integrity of the DNA library fragments and the length of the insert size, and sequencing was performed after the insert size met the expectations. After the libraries passed the test, different libraries were pooled into owcells according to the effective concentration and the target downstream data volume, and cBOT clusters were formed, and then sequenced using the PE250/PE150/PE300 modes of the Illumina Novaseq 6000/Miseq High-Throughput Sequencing Platform.