Red light - Day 3 - DNA - Replicate 2
Tissue samples were homogenized using 3 mm tungsten carbide beads in a TissueLyser II (Qiagen). Genomic DNA was extracted using the E.Z.N.A. HP Plant and Fungal DNA Kit (Omega Bio-Tek), with the optional addition of 10 L 2-mercaptoethanol and 2 L RNase A. DNA concentration was measured with a Qubit (Thermo Fisher Scientific) fluorometer, and quality was assessed using a NanoDrop (Thermo Fisher Scientific) spectrophotometer and gel electrophoresis. Whole-genome bisulfite sequencing (NovaSeq 6000 PE150) was performed by Novogene.