16S sequencing of variable regions V3 and V4 of human saliva from Alzheimer patients and healthy controls
DNA-extraction with ZymoBIOMICS DNA/RNA Miniprep Kit (Zymo Research; article number: R2002); library preparation was performed according to the Illumina 16S metagenomics protocol (Part #15044223 Rev. B) using the Nextera XT Index kit (Illumina Inc. San Diego, CA, USA)