ScRNA-seq of colonic TCRb+CD4+ T cells
Single-cell suspensions were mixed with beads containing unique molecular identifiers (UMIs) and cell barcodes, then underwent sequencing library construction using Chromium Single Cell 3 Library Kits (10x Genomics). Sequencing was performed in a pair-end (2  150 bp) format on an Xplus (Illumina)/MGI DNBSEQ-T7.