16S rDNA of mice
Total DNA was extracted from feces samples. Primers were designed according to the conserved region, and a sequencing junction was added at the end of the primers. Polymerase chain reaction amplification was then performed and the products were purified, quantified, and homogenized to form a sequencing library. The built library was subjected to quality control and then sequenced using an Illumina HiSeq 2500. The original image data were obtained and transformed into raw sequences (sequenced reads) by base calling.