HiFi sequencing of CYXGZ: leaf
Genomic DNA extracted via CTAB underwent quality assessment. A 15 kb library was constructed using the SMRTbell Express Template Prep Kit 3.0 (Pacific Biosciences, CA, USA), involving DNA shearing, damage repair, end repair, hairpin adapter ligation, size selection, and library purification. Following quality control, the resultant SMRTbell library was sequenced on a PacBio Revio platform (Pacific Biosciences, CA, USA) using a single 25M SMRT Cell.