FOXA2_ChIP-seq of mus musculus: colonic crypts_rep1
Chromatin from isolated colonic crypts was immunoprecipitated with either FOXA1 or FOXA2 antibodies. Libraries were prepared with NEBnext kit for Illumina: DNA samples were blunted and a single 3-end A base was added. Adapters were ligated to the ends of the modified DNA and size selection of 200-bp fragments was performed via PAGE extraction, followed by PCR amplification of 18 cycles.