Human single cell sequencing of synovial fluid in the temporomandibular joint (TMJ).
Sequencing library was prepared following the protocol for Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (Dual Index) from 10x Genomics. Cell samples were AOPI stained and number of cells were counted on Nexcelom fluorescent automatic cell counter Cellometer K2, with live cell number 454 cells/uL. 33uL of cell sample was loaded onto a Next GEM chip and processed in Chromium iX, targeting 8000 cells recovery. The final 3' gene library was analyzed on Agilent Tapestation D1000, with a single peak at 509bp, then quantified by Qubit and finally sequenced on Illumina NovaSeq 6000 100 cycle flow cell.