Bifidobacterium animalis subsp. lactis
Nanopore libraries were prepared using the ONT Ligation Sequencing Kit (Oxford Nanopore Technologies) as follows: Genomic DNA integrity was assessed by gel electrophoresis and quantified using a Qubit fluorometer. The DNA underwent end-repair and A-tailing reactions, after which sequencing adapters and motor proteins were ligated to both ends. Unligated molecules were removed via purification steps, and libraries meeting quality standards were sequenced on the PromethION platform.