CO2_fibro_D3_C4_r4
Samples were harvested at 3 and 14 days after transfection of primary Epidermolysis bullosa patient fibroblasts with RNPs (containing Cas9 complexed with sgRNA C2, C4 or nothing as control; see below)  and ssODNs (i.e. sequence of chr. 3: 48,570,186 – 48,569,987; GRCh38.p13; with 4 silent mutations for genotyping). Genomic DNA was purified using the Quick DNA miniprep kit (Zymo Research D3025) according to the manufacturer's recommendations and used as a template for PCR using following primers: Fw 5' CCTCTGAGTCAATGAACCTAATG 3' and Rw 5'  CTACAGGAACCAGGGCAGTG 3'. Four technical replicates were produced per group (i.e. sgRNA C2: GGGAUCCACCGUGAGUCCUC, sgRNA C4: ACUCACGGUGGAUCCCGCUG, and control) at each time point and amplicons were sequenced by Novogene USA on the NovaSeq X Plus lane with the 10B flow cell