Mutational spectrum of breast cancer by shallow whole-genome sequencing of cfDNA and tumor gene panel analysis
The library preparation of tumor tissues was done with a xGenTM Pan-Cancer Hybridation Panel (IDT), which covers 176 genes significantly mutated in cancer (800 Kb of protein-coding bases). Pair-end sequencing was performed on an Illumina HiSeq 2500 for 2X 150 cycles at Novogen, Sacramento, California.