RNA-Seq of Homo sapiens: 46,XY skin fibroblast; CRISPRi targeting experiment
Guide RNAs were transduced into stably expressing dCas9-KRAB fibroblasts; cells were collected using puromycin (Sigma) at 24 hours post infection and washed at 72 hours.  RNA was extracted with the RNeasy Mini Kit (Qiagen). RNA sequencing libraries were prepared using the KAPA mRNA HyperPrep Kit V2 (Roche).