Ninth technical replicate of a 2Lt_0_Tengl2 amplicon library from the control pool 6 of flies used to assess assay accuracy and variance
Ninth technical replicate of a PCR-based amplicon library targeting 2238742-2238967 on chromosome 2L (D. mel. reference genome release 5), prepared for Illumina paired-end read sequencing in order to assay the frequency of In(2L)t. Pooled individuals were used for DNA extraction as described (lightly modified from Langley et al., 2011). Libraries were prepared following a lightly modified version of the Illumina metagenomic amplicon protocol (Illumina, 2013) using custom oligos ordered from IDT. Sequencing was conducted at the Next Generation Sequencing Core of University of Wisconsin Madison Biotechnology Center (https://dnaseq.biotech.wisc.edu), using the Illumina NovaSeq6000 to generate 150bp paired end reads to a depth of at least 20000.