RNA-Seq of the cell line
Strand-specific RNA-seq libraries were prepared from total RNA (TRIzol-extracted) using VAHTS Universal V6 RNA-seq Kit. Three biological replicates per group were analyzed. Libraries were sequenced with 150 bp paired-end reads on Illumina NovaSeq 6000 (50M reads/sample). Raw data were processed with fastp (v0.20.0) for adapter trimming, quality filtering (Q20), and length selection (50 bp).