Raw reads: Glib4 RNA-seq
For the library preparation, total RNA quantity and integrity were re-assessed using Quant-IT RiboGreen RNA Assay Kit (Invitrogen, Carlsbad, CA, USA) and Agilent Tapestation 2200 RNA Screentape. Purification of mRNA, generation of double stranded cDNA and library construction were performed using TruSeq® Stranded mRNA HT (RS-122-2103) with minor modifications to manufacturer specifications. The following custom primers (25µM each) were used for the PCR enrichment step: Multiplex PCR primer 1.0 5'-AAT GAT ACG GCG ACC ACC GAG ATC TAC ACT CTT TCC CTA CAC GAC GCT CTT CCG ATC T-3' Index primer 5'-CAA GCA GAA GAC GGC ATA CGA GAT CAG TGA CTG GAG TTC AGA CGT GTG CTC TTC CGA TCT-3'. Indices were according to the eight bases tags developed by WTCHG (Lamble et al, BMC Biotechnol, 2013). Amplified libraries were analysed for size distribution using the Agilent Tapestation 2200 D1000. Libraries were quantified using Picogreen and relative volumes were pooled accordingly.