RNA-seq of the apex shoot: Pak choi 20 days after germination
After the samples were qualified, the library was constructed. The main process was as follows:(1) Eukaryotic mRNA was enriched by magnetic beads with Oligo (dT);(2) The Fragmentation Buffer was added to randomly interrupt the mRNA.(3) The first cDNA strand and the second strand were synthesized and purified from the mRNA.(4) The purified double-stranded cDNA was subjected to end repair, A-tail addition, sequencing adaptor, and AMPure XP beads for fragment size selection.(5) cDNA library was obtained by PCR enrichment.