GSM8886427: WT mESCs, RNAseq 7 days 2i, replicate 2; Mus musculus; RNA-Seq
Cells were resuspended in TRIzol (ThermoFisher) following manufacturer's instructions and total RNA was isolated by isopropanol precipitation. RNA samples were treated with DNAse using Kit TURBO DNA-freeTM (Invitrogen) following manufacturer's instructions. Libraries were generated by DNBSeq (Illumina) at BGI Tech Solutions.