GSM9016770: PKP2 mutant hiPSC-cardiomyocytes rep2; Homo sapiens; RNA-Seq
hiPSC-cardiomyocytes were dissociated using TrypLE™ Select Enzyme (10x), no phenol red (Gibco: A1217703) and single-cell sorted using the FACSJazz™ Cell Sorter (BD Biosciences) into 384-well plates provided by Single Cell Discoveries. After sorting, plates were centrifuged at 4 °C, put on dry ice and stored at -80 °C until they were processed for library preparation. The plates were prepared for single-cell mRNA sequencing by Single Cell Discoveries according to an adapted version of the SORT-seq protocol.