ATAC-seq of Schisotosoma joponicum:Sporocyst_2
ATAC-seq libraries were constructed following the protocols previously reported. In brief, each sample was pulverized into a fine powder using liquid nitrogen. Then the nuclei were isolated and employed for library construction using the Novoprotein Chromatin Profile Kit (Novoprotein, #N248). After prompt resuspension of the isolated nuclei in the Tn5 transposase reaction mix, the transposition reaction was incubated at 37C for 30 minutes. Equimolar amounts of Adapter 1 and Adapter 2 were then added before the PCR amplification. The libraries were purified using AMPure beads and the quality was assessed by Qubit. The sequencing was performed by Novogene Technology Inc. (Tianjin, China) on an Illumina Nova6000 platform, generating 150 bp paired-end reads.