GSM8783429: Bone marrow, BMT-3; Mus musculus; RNA-Seq
Samples were ground under liquid nitrogen in a mortar and pestle, and the resulting powder dissolved in TRIzol™ Reagent (Invitrogen, Carlsbad, CA, USA), then extracted total RNA. 0.1-1 μg of total RNA per sample was prepared for library preparation. The ABclonal Poly(A)mRNA Capture Module and ABclonal Fast RNA-seq Lib Prep Kit V2 were used for mRNA isolation and library construction following the manufacturers' protocols.