RNA-Seq of Aspergillus flavus  osaA deletion mutant replicate 4
Plates containing 25mL of liquid potato dextrose broth (PDB) were inoculated with 10 spores/mL of A. flavus CA14 wild type, osaA and osaA complementation strain incubated in the dark at 30C for 72 hours. Following incubation, mycelia was harvested, frozen in liquid nitrogen, and lyophilized. Total RNA was extracted from the lyophilized mycelia using the RNeasy Plant Mini Kit (Qiagen, Germantown, MD, USA).