Neurospheres from patient OS11 (region 5, replicate 3)
RNA was extracted from a snap-frozen tissue or cell line pellets with TRIzol Reagent (ThermoFisher, Cat#15596026) and Direct-zol RNA Mini-Prep Plus kit (Zymo Research, Cat#R2071). Libraries were prepared using NEBNext Ultra 2 kit with rRNA depletion module1 (New England Biolabs)