GSM9125217: Control_Rep1_IgG; Homo sapiens; OTHER
CUT&Tag assays were performed using the CUT&Tag Assay Kit (Cell Signaling Technology, #77552) according to the manufacturer's instructions. CUT&Tag assays were performed using the CUT&Tag Assay Kit (Cell Signaling Technology, #77552) according to the manufacturer's instructions. 5×10^4 cells were immobilized on Concanavalin A-coated magnetic beads and sequentially incubated with primary antibodies specific to H3K4me1 and IgG followed by a secondary antibody provided in the kit. pA-Tn5 was then added to facilitate targeted tagmentation of chromatin. After tagmentation, DNA was extracted and purified using the spin columns included in the kit. Indexed libraries were generated by PCR amplification and subsequently purified for next-generation sequencing. Quality and concentration of the libraries were measured by Agilent 2200 Bioanalyzer and High Sensitivity D1000 Screen Tape (Agilent Technologies), respectively. Pooled libraries were sequenced by NovaSeq X Plus (Illumina).