GSM9126748: Sample 5_NHA cells, v117.28, biol rep1; Homo sapiens; RNA-Seq
RNA was isolated 24 h after transfection with the RNeasy MinElute Kit according to manufacturer's protocol, treated with DNase I, incubated with an RNA strand reverse complementary to the respective ON and heated to 95 °C for 3 min and purified again with the RNeasy MinElute Kit. 25 µl of purified RNA (≥ 40 ng/µl was used for poly(A)+ mRNA sequencing). Purified RNA was delivered to CeGaT (Germany) for poly(A)+ mRNA sequencing.The library was prepared from 100 ng RNA with the TruSeq Stranded mRNA Library Prep Kit (Illumina, USA) and sequenced with a NovaSeq 6000 (50 Million reads, 2 × 100 bp paired end, Illumina, USA). Each sample was prepared as a biological duplicate.