GSM7372756: Rheumatoid Arthritis (RA) Human Synovium_FGRA-RA2; Homo sapiens; OTHER
Frozen synovial tissues specimens were provided by Pini Orthopedic Hospital, University of Milan Italy. Tissues sections were generated by cryosectioning and fixed prior to permeabilization and reverse trascription to obtain cDNA for library generation Visium Spatial Gene Expression libraries comprise standard Illumina paired-end constructs that are flanked with P5/P7, necessary for binding to the Illumina flow cell. TruSeq Read 1 is used for priming and sequencing the 16 bp Spatial Barcode and 12 bp UMI, and TruSeq Read 2 is used for priming and sequencing the cDNA insert. The two 10 bp sample indexes are sequenced in the i5 and i7 read respectively. Sequencing these libraries produce a standard Illumina BCL data output folder. SP_28x10x10x90_Dual Index library piered-end, dual indexed sequencing. P5 (index i5:10) - TueSeq Read1 (Spatial BC+UMI+poly(dT)VN) - TueSeq Read2 (2:90 insert) - P7 (Index i7:10)