GSM8381462: Odz4-ICD Tet (+); Mus musculus; RNA-Seq
EBs cultured for 2, 2+3, 2+5, and 2+7 days were washed using HBSS by 2 times then dissociated with collagenase B for 1 hour. Then differentiation medium (DMEM supplemented with 20% fetal bovine serum, 1× non-essential amino acids solution (Gibco), 1 mM sodium pyruvate solution (Sigma-Aldrich), 10-4 M 2-mercaptoethanol (Sigma-Aldrich), 100 units/ml penicillin-streptomycin (Gibco)) was added and pipetting gentally. Dissociated cells were filted with 40um cell strainer and washed and resuspend in PBS with 0.04% BSA. The cDNA library was prepared using the Chromium Next GEM Single Cell 5' Kit v2 for Dual Index (10× Genomics) following the protocol outlined in the 10× Genomics Single Cell Protocols and Cell Preparation Guide.