GSM9045503: Tibialis anterior muscle, sham control mouse#3, Day 1; Mus musculus; RNA-Seq
Total RNA was extracted from tibialis anterior muscle harvested from sham or septic mice using Isogen (Cat# 311-02501, Nippon Gene, Tokyo, Japan) according to the manufacturer's instructions. Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA, Cat No.7760) following manufacturer's protocol and index codes were added to attribute sequences to each sample. Finally, The resulting libraries were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system. The purified libraries were sequenced on a Novaseq 6000 (Illumina) with a 150-base pair-end read setting.