GSM9096922: OCI-AML-3 WT1 cells, replique 2; Homo sapiens; RNA-Seq
Total RNA was extracted from one the OCI-AML3 parental cell line and two KOs (KO clone #1 and KO clone #2). Each experimental condition includes three biological replicates using the RNeasy® Mini Kit (Qiagen; 74104) Libraries were prepared with rRNA depletion with a Kapa mRNA HyperPrep kit (Roche, #KR1352-v7.21) and sequenced using the Illumina Novaseq6000 platform, 100-bp paired-end reads, at 50 million reads per sample and Nextseq500, 75-bp paired-end reads, 30 million reads per sample.