GSM8383344: bonobo-iPS-0009M-B1; Pan paniscus; RNA-Seq
Total RNA of bonobo and chimpanzee iPSCs was extracted using ISOGEN (317-02503; Nippon Gene, Tokyo, Japan) and Direct-zol RNA Miniprep (R2050; Zymo Research, Irvine, USA). cDNA libraries were prepared using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (E7420; New England Biolabs) and sequenced on Illumina NovaSeq with 2 x 150 bp paired-end read configuration.