ZFP36 Inhibits Gastric Cancer Progression by enhancing expression of LIPG and binding its 3'UTR of RNA
ZFP36 Inhibits Gastric Cancer Progression by enhancing expression of LIPG and binding its 3'UTR of RNA
Jurkat and Molt4 cells were grown at 37℃, 5% CO2 in RPMI-1640 medium (Gibco, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, USA) and 1% penicillin/streptomycin, respectively. The GENE-bound RNA was isolated from the immunoprecipitation of anti-GENE using Trizol (Invitrogen). Complementary DNA (cDNA) libraries were prepared with the KAPA RNA Hyper Prep Kit (KAPA, KK8541) according to the manufacturer's procedure