Illumina TruSight Oncology 500 sequencing (FASTQ) of primary gastrointestinal stromal tumors (GIST) and matched patient-derived xenograft (PDX) models across multiple passages.
Genomic DNA (40 ng) was processed using the Illumina TruSight Oncology 500 assay (Cat. No. 20028213) following the manufacturer's protocol. DNA was enzymatically fragmented, end repaired, and A-tailed, followed by ligation of adapters containing unique molecular identifiers (UMIs). Libraries were amplified with index primers, then enriched for >500 cancer-associated genes through two rounds of hybrid capture using biotinylated probes and streptavidin magnetic beads. Captured libraries were purified, quantified with Qubit, and assessed for size distribution by Bioanalyzer. Final libraries were sequenced on the Illumina NextSeq 500 platform.