Sequel II sequencing of Sepia officinalis
Genomic DNA was extracted from flash-frozen brain tissue with the MagAttract HMW DNA kit (Qiagen, Cat. no. 67563) and the sequencing library was prepared with the SMRTbell express template prep kit 2.0 (PacBio, Cat. no. 100-938-900). The library was sequenced on 5 SMRT cells on the PacBio Sequel II with the Sequel II binding kit 2.2 (102-089-000), Sequel II sequencing kit 2.0 (101-820-200) and SMRT Cell 8M tray (101-389-001).