Whole genome sequencing of Onchocerca volvulus
DNA from adult worms was extracted using the Isolate II Genomic DNA kit (Bioline, London, UK) per the manufacturers instruction. Libraries were constructed using the NEBNext library kit per manufacturer's instructions (New England Biolabs, Ipswitch, Massachussets). Raw sequence data was trimmed using trimmomatic and mapped using bwa to the O.volvulus nuclear and mitochondrial genome, the Wolbachia endosymbiont, and Homo sapiens. All reads that mapped to Homo sapiens were removed prior to uploading to the SRA.