16S of symbiotic Oculina arbuscula
To identify bacterial communities associated with symbiotic and aposymbiotic fragments, one symbiotic and one aposymbiotic fragment from five genotypes were flash frozen, and a subsample was preserved in ethanol for 16S metabarcoding (N=10). Metabarcoding libraries were generated using a series of PCR amplifications for the V4/V5 region of the bacterial 16S rRNA gene. Five negative controls were prepared and used to remove contaminating sequences (not provided). Samples were sequenced on an Illumina Miseq at Tufts University Core Facility (paired-end 250 bp).