GSM8676798: TDXd Xenograft F2.3; Homo sapiens; RNA-Seq
Tumor tissue was placed in a round bottom eppendorf and lysed on a TissueLyser II machine (Qiagen) for 2 min at 30 Hz with 350 uL Buffer RLT plus with 0.5% v/v Reagent Dx (Qiagen #19088) and a 5 mm stainless steel bead (Qiagen #69989) per tube. Total RNA was extracted from the homogonized lysate using the RNeasy Protect Cell Mini Kit (Qiagen) as per manufacturer's protocol. Illumina TruSeq mRNA sequencing libraries were prepared by sequencing core (VANTAGE, Vanderbilt Technologies for Advanced Genomics).