shotgun metagenomics of gut microbiome
DNA fragmented by Adaptive Focused Acoustics using a Covaris system. Library construction, including end repair, A-tailing, and adapter ligation, was performed with the GeneRead DNA Library I Core Kit (Qiagen). Custom barcode adapters were substituted for the GeneRead Adapter I Set, and library amplification was carried out using custom index primers based on a protocol adapted from the Illumina multiplexing sample preparation oligonucleotide Kit. HiSeq 4000 sequencer (Illumina)