GSM8346096: Hi-C of E.coli :MG1655 + DPAG 50 uM (15min) Paris; Escherichia coli; Hi-C
Cell fixation was performed with 4% formaldehyde (Sigma-Aldrich, Cat. F8775) as described in Cockram et al. (2021)41. Quenching of formaldehyde with 300 mM glycine was performed at 4°C for 20 min. Hi-C experiments were performed with the Arima kit. Samples were sonicated using Covaris (DNA 300bp). Preparation of the samples for paired-end sequencing was performed using Invitrogen TM Colibri TM PS DNA Library Prep Kit for Illumina according to manufacturer instructions. The detailed protocol is available in Cockram et al. (2021)