GSM8885671: C666-1, BAY4; Homo sapiens; RNA-Seq
Total RNA was extracted from cells using TRIzol reagent. RNA concentration and purity were determined by spectrophotometry, and RNA integrity was detected using an Agilent 2100 Bioanalyzer, ensuring RIN values >8.0. The NEBNext Ultra RNA Library Prep Kit for Illumina was used to construct mRNA libraries. Steps included mRNA enrichment, RNA fragmentation, cDNA first and second strand synthesis, end repair, adapter ligation, fragment selection, and PCR amplification.