GSM8817744: M1 CT; Mus musculus; RNA-Seq
RNA was extracted using NucleoSpin RNA XS kit (Macherey-Nagel). 5ng RNA of each sample was used for library construction cDNA library was generated according the the SMART-Seq2 protocol. cDNA tagmentation was performed using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme, TD501). Circular ssDNA library was generated using MGIEasy Universal Library Coversion Kit (App-A) (MGI, 1000004155)