GSM7656296: female nymph, whole body, rep1; Ericerus pela; RNA-Seq
Samples were homogenized in the PBS, and then filtered through a cellular sieve. The cell viability of the filtrated cell suspension was examined under a fluorescence microscope. Single-cell suspension was loaded into a BD Rhapsody cartridge, and single-cell mRNA capture was achieved by a cartridge with > 200,000 microwells and magnetic beads with barcoded capture oligos. Then, beads were collected for subsequent cDNA synthesis and library construction following the BD Rhapsody single cell 3' whole transcriptome amplification (WTA) workflow. Finally, the libraries were quantified using the Agilent 2100 Bioanalyzer and the Qubit 4.0 and were sequenced on Illumina NovaSeq 6000 with 300-bp reads.