Sonicated, sample 1592
Nave B cell nuclei DNA was sonicated to ~150 bp fragments using a Bioruptor Pico and enriched for 5-hydroxymethylcytosine (5hmC) using the hMe-Seal method. Fragmented DNA was end-repaired and adapter-ligated using the KAPA HyperPrep kit. 5hmC residues were glucosylated with T4 -glucosyltransferase and labeled via azide-alkyne click chemistry with biotin, followed by enrichment of labeled fragments using streptavidin beads. Enriched DNA was used for library preparation with the NEBNext Ultra II DNA Library Prep Kit and NEBNext Multiplex Oligos for Illumina. Libraries were quality-checked by TapeStation and Qubit and sequenced on an Illumina NovaSeq 6000.