gut of mouse
The Amplicon quality was visualized using agarose gel electrophoresis. ThePCRproductspurified with AMPure XP beads (Agencourt) and amplified for another round of PCR. Afterpurifiedwith the AMPure XP beads again, the final amplicon was quantified using Qubit dsDNAAssayKit(Thermo Fisher Scientific,USA). The concentrations were then adjusted for sequencing. Sequencingwas performed on an Illumina NovaSeq 6000 with 250 bp paired-end reads. (Illumina Inc., SanDiego,CA; OE Biotech Company; Shanghai, China).