GSM9006263: B10mdx-6wk-2; Mus musculus; RNA-Seq
Total RNA was extracted using TRIzol RNA isolation (Life Technologies) from frozen muscle samples. RNA was purified using RNeasy mini elute columns (Qiagen) and DNAse treated using Turbo DNA-free kit (Invitrogen). Purified, DNAse-treated RNA was quantified by NanoDrop and quality was assessed using Qbit RNA Assays (Thermofisher) and BioAnalyzer nano chips (Agilent) (RIN>7.8, Average 8.30.32). RNAseq library preparation and sequencing was performed using the TruSeq mRNA stranded kit (Illumina) and the Illumina HiSeq4000 Flow Cell with an average coverage of 63.05 million read pairs per sample at 2x75 base pair read length.