Genomic reads generated through a double-digestion restriction-site associated DNA sequencing protocol (ddRADseq)
We extracted DNA using the Qiagen DNeasy Blood & Tissue kit following the manufacturers instructions. DNA was processed using the double-digestion restriction-site associated DNA sequencing protocol (ddRADseq, Peterson et al. 2012) as described in Mastretta-Yanes et al. (2015) and Garca-Olivares et al. (2019). In brief, DNA was digested with the restriction enzymes MseI and EcoRI (New England Biolabs, Ipswich, MA, USA). Genomic libraries were pooled at equimolar ratios and size selected for fragments between 200-250 base pairs (bp) and, then, sequenced in a single-end 100-bp lane on an Illumina HiSeq2500 platform (Lausanne Genomic Technologies Facility, University of Lausanne, Switzerland).