GSM8565259: ATAC-seq Wiild type, mouse 3; Mus musculus; ATAC-seq
Lin- cells were lysed and incubated with Tn5 rtransposase at 37 degree for 1hr. Genomic DNA was eluented then purified with Ampure beads (Agencourt AMPure XP- Beckman Coulter). Recovered tagmented DNA was amplified and barcoded by PCR performed with KAPA HotStart Taq enzyme (Kapa Biosystems). sequencing library was constructed using NEBNext Ultra II DNA library prep kit for Illumina