GSM8851315: Bmsc biol rep 2; Rattus norvegicus; RNA-Seq
RNA was harvested usingTRIzol reagent (Takara Biotechnology, Japan). 1.3 ug of total RNA was used for the construction of sequencing libraries. The mRNA was isolated and fragmented using mRNA Capture Beads (VAHTS mRNA Capture Beads, N401). The resultant RNAs samples were reverse-transcribed for library construction with NEB Next® VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina, NR604. The libraries were purified by DNA clean beads (VAHTS DNA Clean Beads, N411) for size selection for 200-300 bp fragments. and analyzed on Agilent 2100 Bioanalyzer (Agilent) for measuring fragment size distribution. The qualified libraries were sequenced on Illumina Nova Seq 6000.