Fecal metagenomic seq: AD08
The DNA was extracted from fecal samples using the CTAB method, and the extracted DNA was subjected to concentration, purity, and integrity testing. Qualified DNA was randomly sheared into fragments of approximately 350 bp using an ultrasonic disruptor. Subsequently, the fragments underwent end-repair, polyA tailing, addition of sequencing adapters, purification, and PCR amplification to construct the library. The PCR products were purified using the AMPure XP system, and the insert size of the library was detected using an Agilent 2100. Quantitative analysis of the library concentration was performed using real-time PCR.