GSM8381124: M2A1_CMd15_2; Homo sapiens; RNA-Seq
total RNA was extracted using TRIzol and purified using the Direct-zol RNA MiniPrep kit according to the manufacturer's instructions. RNA concentration was measured with a Qubit fluorometer (Invitrogen), and RNA quality was determined using a TapeStation (Agilent Technologies). RNA-seq libraries were prepared from 500 ng of total RNA using the KAPA RNA HyperPrep Kit with RiboErase according to the manufacturer's instructions. Libraries were pooled and sequenced on a NovaSeq 6000 or NovaSeq X Plus using a 150-cycle kit (paired end, 2 x 150 bp).