GSM9553918: CD45_WTA_Library_A; Mus musculus; RNA-Seq
Tumor-infiltrating leukocytes were isolated from AKPS tumor-bearing mice and labeled using the BD Mouse Single-Cell Multiplexing Kit. After pooling the samples, 30000 CD45+ lymphocytes were FACS-sorted as CD45+ cells displaying lymphocytic morphological parameters and processed with the BD Rhapsody Express Single-Cell Analysis System. cDNA libraries were prepared with the mRNA WTA and Sample Tag protocol (BD Biosciences).