RNA-Seq
Total RNA was extracted from rat liver tissue using TRIzol Reagent. High-quality RNA (RIN  6.5, OD260/280 = 1.82.2) was used for library construction. The stranded RNA-seq transcriptome library was prepared using Illumina Stranded mRNA Prep Kit (Illumina, San Diego, CA) with polyA selection and random hexamer priming. Libraries were size-selected for ~300 bp fragments and amplified by PCR. Paired-end sequencing (2  150 bp) was performed on the NovaSeq 6000 platform.