GSM8502635: Ribo-seq_WT2; Mus musculus; OTHER
For RNA extraction, we employed the TRIzol method, a robust approach ensuring the purity and quality of RNA. The lysate solution first underwent nucleic acid digestion and separated ribosome bounding RNA from free mRNA. RPFs were obtained by enrichment of ribosome-mrna complex. After the rRNA was removed and purified by PAGE glue, the target RNA fragments were obtained. End repair, add 5 'and 3' joints directly to both ends. cDNA was synthesized by reverse transcription and PAGE gel was purified. The initial library was obtained by PCR enrichment with sequencing splices.