GSM9195464: USP28 knockout_End_T4; Homo sapiens; OTHER
Genomic DNA was isloated using QIAGEN DNA Blood Maxi kit protocol, from the BRAOD institute gRNAs were amplified with barcoded primers following the “PCR of sgRNAs, shRNAs, and ORFs from genomic DNA for Illumina sequencing”  protocol, with the following P7 barcode primers for the indicated libraries. s_USP28_T0 : A01 (P7_MT01) s_USP28_T4 : A02 (P7_MT02) s_WT-T4 : A01 (P7_MT01) s_P53-T4 : A02 (P7_MT02) s_WT-T0 : A07 (P7_MT07) s_P53-T0 : A08 (P7_MT08)