RNA-Seq of apple calli
Transcriptome sequencing was performed on the control and PpSAMS-overexpressing apple calli respectively. Based on the Illumina sequencing platform, Oligo dT magnetic beads were used to enrich mRNA from the total RNA. After fragmentation, the first-strand cDNA was synthesized using random hexamer primers, followed by the synthesis of the second-strand cDNA. After end repair, A-tailing, adapter ligation, fragment selection, amplification and purification, the library was ready.