Ion Torrent sequencing of P. faecium BR41A from human mucosal-luminal interface aspirates
Single colony grown in 104c PY+X+80mM disodium succinate broth, anaerobically at 37C for 4 days. DNA extracted using Quick-DNA HMW MagBead Kits, with an additional RNase A treatment and two additional g-DNA Wash Buffer washes. Ion Torrent sequencing using Ion Xpress Plus Fragment library kit with enzymatic fragmentation. Libraries were size-selected using AMPure XP beads and templated on an IonChef with 400 base-read templating and sequenced on an Ion GeneStudio S5 System using an Ion 530 chip.