GSM9337586: PC3 siCNT rep2; Homo sapiens; RNA-Seq
Total RNA was extracted from cell pellet using Qiagen RNeasy Plus kit. 1 μg of total RNA was used for the construction of sequencing libraries. NEBNext Ultra II RNA Library Prep Kit for Illumina was used to prepare sequencing libraries. The Kit procedure was performed without modifications. This procedure includes rRNA depletion, remaining RNA purification and fragmentation, cDNA synthesis, end preparation, Illumina adapter ligation, library PCR amplification and validation.