GSM8818175: GBM_3D_5-FU/U and Carmustine_003; Homo sapiens; RNA-Seq
Total RNA was extracted from the 3D encapsulated cells using the Norgen`s Total RNA purification kit, [Norgen Biotek Corporation], following the manufacturer's protocol. Complete cDNA was synthesized with SmartScribe reverse transcriptase using a universally tailed poly-dT primer and template switching oligo. Purified cDNA was amplified with Advantage 2 DNA Polymerase. After ultrasonic shearing of the amplified cDNA (Covaris S2) Illumina fragment library was prepared using NEBnext chemistries (New England Biolabs).