GSM9489114: Control_3; Mus musculus; RNA-Seq
Liver total RNA was extracted according to the instruction manual of the TRlzol Reagent. A total amount of 1 μg RNA per sample was used. Sequencing libraries were generated using Hieff NGS Ultima Dual-mode mRNA Library Prep Kit for Illumina. The libraries were sequenced on an Illumina NovaSeq platform to generate 150 bp paired-end reads, accordingto the manufacturer's instructions. For library construction, 1 μg of total RNA per sample was used. mRNA libraries were prepared according to the manufacturer's protocol using the Hieff NGS Ultima Dual-mode mRNA Library Prep Kit for Illumina (Yeasen, Shanghai, China). Briefly, mRNA was enriched using oligo(dT) magnetic beads, followed by first- and second-strand cDNA synthesis. cDNA ends were blunted, 3′-adenylated, and ligated to hairpin-structured adaptors. Libraries were purified with AMPure XP beads, treated with USER enzyme, and amplified by PCR using indexed primers. Final libraries were purified and quality-controlled using an Agilent Bioanalyzer 2100.