GSM8534761: ATCC 17978 strain, overexpressed TetR2-His-tag, rep3, 3; Acinetobacter baumannii; ChIP-Seq
The cultures were added 1% formaldehyde for crosslinking at room temperature for 20 minutes. After quenching, bacteria were lysed using 2mg/ml lysozyme and sonicated after centrifugation. His-tagged protein-DNA complexes were isolated by nickel magnetic beads and purified by TIANquick Midi Purification Kit (Tiangen) after de-crosslinking. Next generation sequencing library preparations were constructed according to the manufacturer's protocol.