RNA-seq of ExPEC PCN033
The ExPEC PCN033 strain was incubated in fresh mouse serum for one hour. The cells were then harvested, washed, and total RNA was extracted. Ribosomal RNA and genomic DNA were depleted, and the mRNA underwent reverse transcription to synthesize complementary DNA (cDNA). This cDNA was subsequently utilized for sequencing library construction, which included fragmentation, adaptor ligation, and enrichment. Finally, the library was subjected to high-throughput sequencing using the Illumina NovaSeq 6000 platform.