Total RNA was extracted using the RNeasy Plus universal kit (Qiagen, Germantown, MD, USA) according to the manufacturer&#39;s protocol. cDNA was synthesized from 1 &micro;g of total RNA using the RT2 First Strand Kit (Qiagen, Germantown, MD, USA), following the manufacturer&#39;s guidelines. Stranded sequencing libraries were prepared from ribo-depleted RNA preparations using the KAPA Stranded mRNA kit.