GSM8502928: organoids from caRANKvil-Tg_1; Mus musculus; RNA-Seq
Total RNA isolation was performed using the RNA isolation kit (VBCF, Vienna) which uses a lysis step based on guanidine thiocyanate and magnetic beads applied on the KingFisher instrument. Equivalent quantities of total RNA were used for library preparation using the Lexogen QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina. Libraries were pooled at an equimolar ratio and sequenced on an Illumina HiSeq 2500 instrument using the single-read 50-read mode.