GSM8535089: TG-treated CRISPR screen - input - biological replicate 2; Homo sapiens; OTHER
DNA was extracted using phenol:chloroform:iso-amyl alcohol method. See Scarborough et al., 2021 for details. Library amplification was completed following a two-step variation of the BROAD institute protocol (BROAD Institute PCR of sgRNAs for Illumina sequencing). The two step variation of the BROAD institute protocol consists of an initial amplification step using primers flanking the P5 and P7 primers used for sequencing.