Neottia nidus-avis, pool of stem samples
The RNA extracts of leaves and roots of the nine species were polled by species and organ to construct 18 Nanopore sequencing libraries using the cDNA-PCR sequencing kit SQK-PCS109 combined with the barcoding kit SQK-PBK004 (Oxford nanopore technologies, UK) and sequenced on R9.4 MinIon flow cells. Basecalling, demultiplexing and trimming was done using Guppy v4.0. Between 2.88 and 10.22 millions of reads were obtained.