Whole-genome sequencing of Pinus sylvestris: seed megagametophyte
DNA was extracted from seed megagametophyte using E.Z.N.A SP Plant DNA Kit (Omega Bio-tek), fragmented using Bioruptor UCD-200 (Diagenode), and size-selected using Agencourt AMPure XP beads (Beckman Coulter). The library was prepared using NEBNext Library Prep Master Mix Set for Illumina (New England Biolabs) with NEBNext Multiplex Oligos for Illumina (New England Biolabs).