GSM8446942: RGCs from mice treated with AAV2-shScramble, Sample3; Mus musculus; RNA-Seq
The total RNA of each sample was extracted by RNeasy Micro Kit (QIAGEN). The purity and quantification of RNA were assessed with Nanodrop 2000 (Thermo Scientific). The integrity of RNA was evaluated by Agilent 2100 Bioanalyzer (Agilent Technologies). 1ng of total RNA was used for the construction of sequencing libraries. The libraries were constructed using VAHTS Universal V6 RNA-seq Library Prep Kit according to the manufacturer's instructions.