GSM9194784: prf1-/- mouse, Bone marrow, LCMV infected，HLH1; Mus musculus; RNA-Seq
Single-cell cDNA libraries were generated as described in Single cell 3' whole transcriptome amplification (WTA) protocol (BD Rhapsody,USA) using successfully prepared single-cell suspensions.Single-cell suspensions from each sample were processed individually. For each sample, cells were washed three times, and cell count and viability were assessed using a fluorescence cell analyzer (Countstar® Rigel S2, ALIT Life Science, China) with an AO/PI reagent. Subsequently, the cells were loaded onto a cartridge, lysed in situ, and polyadenylated mRNA was captured with oligo(dT) beads according to the manufacturer's protocol. The beads were magnetically separated and underwent on-bead reverse transcription, followed by Exonuclease I digestion. The resulting cDNA was amplified using extension primers and purified with SPRIselect beads (B23318, Beckman Coulter, USA). The quantity of the PCR products was accurately determined by using Qubit 4.0 along with the Qubit dsDNA HS Assay (Q32854, Thermo Fisher Scientific, USA).For the high-throughput sequencing process, the libraries were prepared in strict accordance with the manufacturer's instructions. Subsequently, these libraries were applied to the Illumina Novaseq 6000 system for 150 nt paired-end sequencing.