WGBS of Gasterosteus aculeatus: adult gill
1 g of Stickleback genomic DNA was mixed with 10 ng lambda phage DNA and sheared with ultrasound to the average size of 300 bp. End-repair, dA tailing, and methylated adaptor ligation were performed with NebNext DNA UltraII kit (NEB). After adaptor ligation, libraries were bisulfite converted with EZ DNA Methylation Kits (ZYMO RESEARCH) according to the manufacturers protocol. After conversion, final libraries were amplified with NEBNext Q5U Master Mix (NEB) and sequenced with Illumina HiSeq1500.