GSM8383215: advanced liver tumour AlbR26Met_6; Mus musculus; RNA-Seq
mRNA was extracted with Qiagen kit from the indicated samples, following manufacturer instructions. 500 ng total RNA per sample was diluted in RNase- DNase- and protease- free molecular grade water (maximum volume 20 µL). RNA quality was verified with Agilent bioanalyzer Tapestation with RIN and/or Dv200 values; and purity evaluated with Nanodrop considered as valid when OD260/280 was between 1.8 and 2.2. Samples were submitted to ICM platform for sequencing with Illumina NovaSeq X technology (purification of poly-A containing mRNA molecules; 40 million reads/sample, 100 bases each, paired end). Random primed cDNA library, purification of poly-A containing mRNA molecules, mRNA fragmentation, random primed cDNA synthesis, adapter ligation and adapter specific PCR, amplification