wtDJ1
mRNA was purified using the TruSeq RNA sample preparation kit (http://www.illumina.com/) following the companys protocol. Following purification, the mRNA was fragmented, and first-strand cDNA was generated using reverse transcriptase and random primers (Kim et al., 2024). Libraries were constructed for the leaf of 17 days in over-expression and knock-out OsWRKY36 and wild type. The fragmented cDNA was then purified, amplified via PCR and sequenced with a NovaSeq 6000 device. Repetitions were performed for all samples.  In the initial sequencing, 2.7-0 Gb was sequenced at each end. These results generated 16-43 X 106 paired-end reads. For statistical analysis, two biological replicates of OsWRKY-ox16, wt-IM, oswrky-ko5, and wt-DJ were examined.