GSM9490659: Das_Alyona__BT2015050_2015_A1; Homo sapiens; OTHER
FFPE tissue sections for GeoMx DSP experiments were cut to a thickness of 5μm using a microtome and mounted onto Superfrost Plus slides within 1 month of spatial transcriptomics analysis.  Fresh-frozen tissue sections for Visium (10 μm thickness) were mounted onto Visium CytAssist slides, with two samples per slide. Sample preparation—including H&E staining, imaging, probe hybridization, and library construction—was carried out following the manufacturer's protocol (10X Genomics, Visium Spatial Protocols – Tissue Preparation Guide, CG000240, and Visium CytAssist Spatial Gene Expression for Fresh Frozen – Tissue Preparation Guide, CG000636, Rev A). For GeoMx,sequencing was performed on an Illumina NovaSeq 6000 and fastq files were processed into gene count data for each sample using the GeoMx® NGS Pipeline. Spatial transcriptome sequencing libraries were prepared with 10xGenomics Visium Spatial Gene Expression Slides & Reagent kit according to manufacturer's construction.