ddRADseq of Primula auricula : sample from FR_Sous_Dine locality, individual n 5
We followed the ddRADseq protocol developped by Peterson & al. 2012, with modifications according to our model species. DNA was retrieved with  DNEASY plant mini kit (Qiagen, Hilden, Germany), and digested using PstI-HF and MfeI-HF (NEB) enzymes. Dual indexing with Illumina adapters during PCR amplification. Raw data obtain from the sequencing facility were demultiplexed using Cutadapt. Please refer to our publication (Morelon et al. In progress) for more details