Transcriptome sequencing MRSA sample 1 of lung macrophages from methicillin-treated C57BL/6N mice infected with MRSA
Total RNA was extracted from iBMDMs infected with SA and treated with DMSO or triptolide, and from lung macrophages of mice infected with SA or MRSA treated with PBS, triptolide, triptolide combined with SM-164, or methicillin according to the TRIzol manual (Life Technologies, 15596-026). RNA sequencing was conducted using the Illumina Novaseq 6000 platform with paired-end 150-bp reads.