GSM8848718: KLN205 tumors, sh3 Tmprss11b, biol rep 3; Mus musculus; RNA-Seq
30-50 mg tumor tissue chunks were homogenized using a polytron and total RNA was isolated using Trizol (Ambion by Life Technologies; 15596018), followed by additional cleanup and DNase digestion using RNeasy Mini Kit (Qiagen; 74104) and subsquently used for RNA-seq profiling. Samples are run on the Agilent Tapestation 4200 to determine level of degradation thus ensuring  only high quality RNA is used (RIN Score 8 or higher).   Qubit® 4.0 Fluorimeter from ThermoFisher is used to determine the concentration prior to staring library prep.  One microgram of total DNAse treated RNA is then prepared with the TruSeq Stranded mRNA Library Prep Kit from Illumina.  Poly-A RNA is purified and fragmented before strand specific cDNA synthesis.  cDNA are then a-tailed and UDI adapters are ligated.   After adapter ligation, samples are PCR amplified and purified with AmpureXP beads, then validated again on the Agilent Tapestation 4200.  Before being normalized and pooled, samples are quantified by Qubit then run on the Illumina NextSeq 2000 on a P2-100 flowcell.