Human gut metagenome from MIAB sample 99, Zymo_Kit extraction, replicate 1, Oxford Nanopore
Human gut metagenomic DNA extracted using Quick-DNA HMW MagBead kit (enzymatic lysis), technical replicate 1. Library prepared using Oxford Nanopore Ligation Sequencing Kit (SQK-LSK110) with NEBNext Companion Module and Agencourt AMPure XP beads. Targeted 100-200 fmol DNA in 47 L per sample. Loaded onto PromethION R9.4.1 flow cell with 72-hour run length. Basecalling performed using Guppy v4.0.11 with minimum quality score threshold of 7.