GSM9555963: Hi-C - Anableps dorsal retina, biol rep2; Anableps anableps; Hi-C
Hi-C was performed on freshly bisected dorsal and ventral retina tissue of Anableps anableps (two dorsal and two ventral biological replicas) using the Arima-HiC+ Kit (Arima Genomics, Cat. No. A510008, Carlsbad, CA, USA), following the manufacturer's protocol. Briefly, tissues were crosslinked with formaldehyde and digested with a restriction enzyme cocktail provided in the kit. Proximity ligation was performed to join spatially proximal DNA fragments, followed by purification and fragmentation of the ligated DNA. Biotinylated fragments were enriched and processed for library preparation using the Arima Library Prep Module (Arima Genomics, Cat. No. A303011, Carlsbad, CA, USA). Libraries were sequenced on the NovaSeq 6000 (Novogene Corporation Inc., Sacramento, CA, USA) using 150bp paired-end reads, yielding approximately 600 million read pairs per sample.