GSM9047458: Liver tissue, Mtb-infected Balb/c, 14 days, sample 3; Mus musculus; RNA-Seq
The right lung of the animals was collected and stored in 1.0 mL of Trizol Reagent (Invitrogen, Carlsbad, CA, USA). The RNA was analyzed for its integrity and concentration using the Agilent 2100 bioanalyzer using the RNA LabChip® Kit (Agilent Technologies, USA) to certify that it had the characteristics required to be used in RNA sequencing technology (RNAseq). The RNAseq was performed using the SOLiD Total RNAseq kit (Applied Biosystems). The material was used for the construction of fragment-like libraries, for further sequencing using the kits and protocols recommended by the SOLID™ Platform. Mapping revealed that the coverage achieved by sequencing was 4.5 times the total mouse transcriptome, averaging approximately 7 million reads.