GSM8345861: JM1133; Mus musculus; ssRNA-seq
A portion of mouse mammary tumor was homogenized using Trizol and Qiagen RNeasy Kits to isolate total RNA using a modified extraction protocol. Bulk RNA libraries were generated and  constructed following standard Illumina protocols for RNA sequencing  on an Illumina HiSeq4000 (MaPR172H/- tumors) or Illumina NovaSeq 6000 (MaPR245W/- and MaP-/- tumors) by the the MD Anderson Cancer Center ATGC  Indexed cDNA libraries were minimally PCR amplified, pooled and sequenced. Stranded mRNA-Seq