GSM9486145: P0 Ddx3x-3HA Female Heart DDX3X eCLIP Rep 3; Mus musculus; RIP-Seq
P0 Ddx3x-3XHA/+ female hearts (n=3 replicates, 8 hearts/rep HA eCLIP, n=1 replicate, 4 hearts/rep IgG eCLIP) were dissected and snap frozen. Tissue was cryogenically ground and UV crosslinked. HA and IgG immunoprecipitation were performed. RNAse treatment and subsequent seqeuncing of bound RNA and total RNA input were perfformed. RNA libraries were prepared for sequencing according to published eCLIP protocols and performed by Eclipse Bioinnovations.