RNA-Seq exf EJ - ext5
RNA was extracted using TRIzol (Ambion, Thermo Fisher Scientific, MA, USA; Cat#15596018).Approximately 20 g of RNA with RIN values over 7.0 was sent for RNA library construction and sequencing using Illumina NovaSeq 6000 (PE150 configuration; NovogeneAIT, Singapore) for at least 15 million read pairs.