In this study, the complete mitogenome of P. latericea was firstly determined,
1. With 1  Establishing a DNA initial quantity library; 2. Covaris M220 ultrasound breaks DNA to 300-500bp; 3. Leveling, adding A at the 3 'end, and connecting the index connector (TruSeq)  Nano DNA Sample Prep Kit; 4. Library enrichment, PCR amplification of 8 cycles; 5. 2% agarose gel recovery target band (Certified Low Range Ultra Agarose); 6. Quantitative analysis of TBS380 (Picogrreen), mixed on machine according to data ratio; 7. Perform bridge PCR amplification on the cBot solid-phase carrier to generate clusters; 8. Illumina NovaSeq sequencing platform for 2 * 150bp sequencing.