RNA-Seq HAE
Total RNA from was purified using Direct-zol RNA MicroPrep kit (Zymo Research). mRNAs were enriched from total RNA samples with a poly-A pull-down the library was constructed using Illumina TruSeq chemistry. Libraries were sequenced using the Illumina NovaSeq 6000 (Illumina) at Columbia Genome Center. Samples were multiplexed in each lane, yielding a targeted number of single-end/pair-end 100bp reads for each sample. RTA (Illumina) was used for base calling and bcl2fastq2 (version 2.19) converted BCL to fastq format and adaptor trimming. Pseudoalignment to a kallisto index were created from transcriptomes (Ensembl v96, Human:GRCh38.p12; Mouse:GRCm38.p6) using kallisto (0.44.0).