Submononucleosomal, sample 1590, input
Nave B cell nuclei DNA was digested to subnuclosomal units using micrococcal nuclease. Digested DNA was purified, followed by end-repair and adapter-ligation using the KAPA HyperPrep kit. Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit with NEBNext Multiplex Oligos for Illumina without 5hmC enrichment and used as input controls. Libraries were quality-checked using TapeStation and Qubit and sequenced on an Illumina NovaSeq 6000 at the Institute for Genomic Medicine at Nationwide Childrens Hospital (Columbus, OH).