GSM8988051: Parse sequencing, PC3cisplatin sublibrary4 (contains all samples); Homo sapiens; RNA-Seq
Cells were collected on timepoint day using tubes pre-coated with bovine serum albumin (BSA). Cell fixation was performed on timepoint day using Parse Biosciences Fixation kit (Parse Biosciences) according to manufacturer protocol, except for no cell filtering steps were performed. No cell filtering steps were performed during barcoding and library prep to prevent loss of the large cells surviving chemotherapy. Barcoding and library prep for PC3 cells untreated and surviving cisplatin was done using the Evercode Whole Transcriptome Mega kit according to manufacturer protocol (V2, Parse Biosciences). Sequencing was performed on an Illumina NovaSeq 6000 and alignment done using v0.7.2p alignment pipeline (Parse Biosciences). Barcoding and library prep for PC3 cells untreated and surviving docetaxel was done using the Evercode Whole Transcriptome Mega kit according to manufacturer protocol (V2, Parse Biosciences). Sequencing was performed on an Illumina NovaSeq X and alignment done using v1.0.6p alignment pipeline (Parse Biosciences).