GSM8886613: Dnuc-1 0mM phosphate 4h Experiment 2 Replicate 1; Neurospora crassa; RNA-Seq
Mycelia were filtered using Whatman paper #1 and flash frozen using liquid nitrogen. RNA was isolated by beading beating in Trizol reagent and then cleaned up using the Qiagen RNeasy kit. RNA were prepared for sequencing using poly-A enrichment and standard Illumina protocols