GSM9555717: C4-2B Ola 5-1 5-day; Homo sapiens; RNA-Seq
RNA was isolated from cultured cells using Trizol reagent (Thermofisher) following manufacturer's instructions and further purified using the RNeasy kit (Qiagen) with the optional on-column DNase step according to the manufacturer's protocols. Total RNA was eluted from the columns in nuclease-free water and stored at -80°C Indexed, stranded mRNA-seq libraries were prepared from total RNA (1000 ng) using the KAPA Stranded mRNA-Seq Kit (Roche) according to the manufacturer's standard protocol for mRNA capture, fragmentation, random-primed first strand synthesis, second strand synthesis with dUTP marking, A-tailing, adaptor ligation, and library amplification. Libraries were pooled and multiplex sequenced on an Illumina NovaSeqX Plus System