Transcriptomic time-series of human embryonic stem cell differentiation to hypertrophic-like chondrocytes via a mesenchymal intermediate
Transcriptomic time-series of human embryonic stem cell differentiation to hypertrophic-like chondrocytes via a mesenchymal intermediate
Cells harvested into QIAGEN RLT buffer. Pellets homogenized with molecular grinding resin and plastic pestle. hESC/hiPSC cultured in TeSR™-E8™ on vitronectin. Differentiation to iMSCs via MesenPRO RS™. 3D pellets (200k cells) cultured in medium with TGFβ3 and BMP2. RNA extracted using QIAGEN RNeasy mini kit, as per manufacturers instructions. Library preparation of samples was performed using the Illumina TruSeq Stranded mRNA Sample Preparation Kits (Illumina).