Ctla4 ki cd45+ cells
This study was designed to investigate the transcriptional landscape of intestinal immune cells. CD45 cells were isolated from intestinal tissue using fluorescence-activated cell sorting (FACS). Single-cell RNA sequencing libraries were prepared using the 10x Genomics Chromium platform. Libraries were sequenced on an Illumina platform with paired-end reads to profile gene expression at the single-cell level across different experimental groups (WT, KO, and KI).