RNA-seq of mouse CD4+ and CD8+ cells after fed with SF-NIH31 or SF-AIN93G diet
RNA-seq of mouse CD4+ and CD8+ cells after fed with SF-NIH31 or SF-AIN93G diet
Mice splenocytes were collected. CD44-TCRb+ CD4+ or CD8+ naive T cells were sorted by flow cytometry. Total RNA was extracted using the Rneasey Mini Kit (Qiagen), followed by quality assessment using the TapeStation system (Agilent). Library preparation was conducted by BGI Health (AU)