GSM8045961: p10_Lamin_4; Mus musculus; OTHER
P10 female mice were intraperitoneally injected with PMSG. Oocytes were harvested in M2 medium 48 h after PMSG. The DamID procedure was performed as previously described (Borsos et al., 2019). Briefly, an mRNA mixture containing 100 ng/μl membrane-eGFP, and AID–Dam–lamin B1 or AID–Dam were injected into the cytoplasm of oocytes. Oocytes were isolated and injected with 5 ng/μl AID–Dam–lamin B1 or 20 ng/μl AID–Dam and kept in auxin-free M2 medium for 6–8 h to methylate LADs or accessible regions, respectively. Following oocyte collection, library preparation and sequencing were performed as previously described (Borsos et al., 2019).