whole genome seqence of Aeromonas hydrophila resistant to 100ppm oxytetracycline, replicate 2
A total amount of 1g DNA per sample was used as input material for the DNA sample preparations. Sequencing libraries were generated using NEBNext UltraTM DNA Library Prep Kit for Illumina (NEB, USA) following manufacturers recommendations and index codes were added to attribute sequences to each sample. Briefly, the DNA sample was fragmented by sonication to a size of 350bp, then DNA fragments were end-polished, A-tailed, and ligated with the full-length adaptor for Illumina sequencing with further PCR amplification. Subsequently, PCR products were purified (AMPure XP system) and libraries were analysed for size distribution by Agilent2100 Bioanalyzer and quantified using real-time PCR. Sequencing then was performed on Illumina NovaSeq PE150 platform (Beijing Novogene Bioinformatics Technology Co., Ltd.)