RNA-Seq of homo sapiens: ALI culture
The column-based High Pure RNA Isolation kit (Roche) was used according to the manufacturer's instructions for RNA extraction. To remove residual DNA, a second DNase digestion was performed for 1 hour using amplification grade DNase I (Merck). Subsequently, 200 ng total RNA was transcribed into double-stranded cDNA and amplified by PCR. The sequencing library was generated using the Native Barcoding Kit 24 V14(Oxford Nanopore Technologies) and sequenced on a P2 solo for 72 h.