GSM8988184: A459 cells, scrambled shRNA, air-liquid interface, replicate 3; Homo sapiens; RNA-Seq
Cells were harvested in PBS and pelleted by centrifugation. Pellets containing apporximately 500000 cells were flash frozen and sent to Genewiz (Azenta) for further processing. RNA was extracted using standard protocols for downstream Illumina sequencing. RNA libraries were prepared and poly (A) selection was conducted to remove ribosomal RNA.