GSM9047383: iTF-iPSCs, LPS 100mg/ml + IFNG 20 ng/ml, 24h, rep2 (RNA-seq); Homo sapiens; RNA-Seq
Total RNA was extracted from untreated and LPS+IFNG-treated iTF-iPSCs and iTF-Microglia (three replicates per condition) using the Total RNA Purification Plus Kit (Norgen). Eukaryotic RNA-Seq (non-stranded) libraries were prepared by Novogene. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.