GSM8565173: RT+FTY (biol 2); Mus musculus; RNA-Seq
Tumors were collected in RPMI with 10% FCS and digested with 1 mg/ml collagenase IV (Thermo Fisher Scientific) and 50 µg/ml DNase I (Roche) for 45 minutes at 37°C using a rotating device. The cells were then washed in PBS with 2 mM EDTA and passed through a 70-µm filter (Biologix). Red blood cells were lysed with a buffer (17 mM Tris pH 7.2, 144 mM NH4Cl) for 2 minutes, followed by a PBS wash. Library amplification was carried out following the BD Rhapsody System mRNA Whole Transcriptome Analysis (WTA) and Sample Tag Library Preparation Protocol (Doc ID: 23-24119 (02))