GSM8849315: IP_3_1_Blue_Kontrol; Triticum aestivum; MeDIP-Seq
The genomic DNA samples were isolated with NucleoSpin® Plant II Kit (MACHEREY-NAGEL GmbH, Düren, Germany) according to the manufacturer's instructions. The DNA sequencing library was prepared using immunoprecipitated (12 samples) and pooled, fragmented IP input DNAs (4 pooled samples) with xGen™ ssDNA & Low-Input DNA Library Preparation Kit (Integrated DNA Technologies (IDT), Inc., Coralville, IA, USA) according to the manufacturer's instructions. The normalized, pooled, and denatured library samples were sequenced on the Illumina NextSeq platform (Illumina, Inc., San Diego, CA, United States) using 75 + 75 bp paired-end chemistry with NextSeq 500/550 HiOutput Kit v2.5 (150 Cycles)