GSM9454040: MOLM-13, A485, H3K27me3, rep1; Homo sapiens; ChIP-Seq
Cells were crosslinked with 1% formaldehyde for 10 minutes. Crosslinking was terminated with 125mM Glycine for 5 minutes. Cell pellets were washed twice with ice-cold PBS and snap-frozen in liquid nitrogen ChIP libraries were prepared using NEBNext® Ultra II DNA Library Prep Kit for Illumina. ChIP DNA was amplified using 11 cycles according to the manufacturers instruction. Amplified ChIP libraries were finally size selected using AMPure Beads (Beckman-Coulter) and analyzed via Tapesation (High Sensiticity, Agilent).