GSM8988535: TA Muscle, Fatigue, 2; Mus musculus; RNA-Seq
TA tissue was shipped on dry ice to Admera Health who processed the samples and performed mRNA extraction, sequencing, and analysis. Total RNA was quantified by Qubit RNA HS Assay (ThermoFisher) and RNA integrity was determined by Bioanalyzer 2100 Eukaryote Total RNA Nano (Agilent Technologies, CA, USA) with  RNA integrity numbers (RINs) of ≥ 7.7. PolyA selection and cDNA libraries were constructed using the NEB Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs Inc., Massachusetts, USA). Samples were sequenced on an Illumina NovaSeq (Illumina, California, USA) with a read length configuration of 150 PE for 60M paired-end reads per sample (30M in each direction).