GSM9046075: Roots of six day old seedlings, Rep2; Arabidopsis thaliana; ATAC-seq
Roots of six day old seedlings or A. thaliana Col-0 were cut 0.5 - 0.7 cm from their tip and a total of 180 mg of root tissue was collected. Single cell ATAC-seq libraries were produced from freshly isolated nuclei in accordance with the 10X Genomics Chromium Single Cell ATAC-seq kit protocol (v 1.1 protocol, Rev D), targeting ~14-15,000 nuclei per reaction. The adapter-ligated libraries were amplified using nine PCR cycles.