GSM8885040: WGS_cWX2200_96H_rep2_ChIPinput; Escherichia coli str. K-12 substr. W3110; OTHER
Wild type and mutant cells of Escherichia coli W3110 growing in Hi-Def Azure media suplemented with 0.2% glucose were crosslinked using either 10% formaldehyde for Hi-C or 3% formaldehyde for ChIP-seq and then lysed by treatment with 4 mg/ml lysozyme and 1% SDS as previouly described (Wang et al., 2022 PMID: 35101983). The solubilized chromatin was digested with Sau3AI for 2 hours at 37°C. Standard library construction protocol was used for Illumina Nextseq500/Nextseq2000 sequencing platforms. Illumina Truseq indexes were used.