GSM9453990: GA0518 cells, untreated, biol rep 1; Homo sapiens; RNA-Seq
Total RNAs were isolated and purified using the RNeasy Mini Kit (Qiagen, 74106) and converted to cDNA using Luna® Universal One-Step RT-qPCR Kit (New England Biolabs, E3005). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Strand specific library