Crude glycerol and sanitary sewage anaerobic co-digestion in anaerobic fluidized bed reactors aiming at the production of hydrogen and methane.
For microbial community analysis, biomass samples were collected from the reactors, washed with phosphate buffer, and then centrifuged at 6000 rpm. The resulting biomass pellets were stored at -20 C. DNA was extracted from these pellets using the FastDNA SPIN Kit for Soil (MP Biomedicals), following the manufacturers instructions.Subsequently, PCR amplification was performed to increase the number of copies in the amplicon library for easier detection. The resulting fragments were sequenced using the Illumina NovaSeq6000 PE 250 platform, and the reads were mapped against the 16S rRNA reference database (Devens et al., 2024). The selection of samples for diversity analysis aimed to correlate the predominance of microorganisms with shifts in metabolic pathways responsible for H2 and CH4 production.