Single-cell data of testicular organoids cultured with the traditional system on the sixth day.
Single-cell RNA-seq library is constructed using SeekOne kit. It involves steps like reverse transcription, droplet generation, cDNA purification and amplification, adapter ligation, and index PCR. The library is analyzed with Qubit and Bio-Fragment Analyzer and sequenced on Illumina NovaSeq 6000 with PE150 read length.