DNA-seq of Vespa soror: adult female thorax tissue
For in-house ONT sequencing, 1.5 g of gDNA was purified using AMPure XP magnetic beads (A63880; Beckman Coulter, USA). An ONT library was prepared from 500 ng of purified gDNA using the NEBNext Ultra II End Repair/dA-Tailing Module (E7546, New England Biolabs, USA) and the Ligation Sequencing Kit SQK-LSK109 (ONT, UK). Sequencing was performed using a MinION device with an R9.4.1 MinION flow cell (FLO-MIN106, ONT, UK).