PacBio Genome Sequencing of Pategonian toothfish
Using the covaris G-tube we generated 20Kb fragments by shearing genomic DNA according to the manufacturers recommended protocol. Using the AMpureXP bead purification system to remove the small fragments. The SMRTbell library was sequenced using SMRT cells (Pacific Biosciences, Sequel SMRT Cell 1M v2) using sequencing kit (Sequel Sequencing Kit 2.1) and 600 minute movies were captured for each SMRT cell using the Sequel (Pacific Biosciences) sequencing platform.