GSM8486915: patient 046 metastasis 1; Homo sapiens; RNA-Seq
RNA from patient-derived samples was isolated according to an acid guanidinium thiocyanate-phenol-chloroform extraction method (PMID: 2440339). The TRIzol incubation time for patient samples was increased to 10min and the samples were regularly resuspended at room temperature Library preparation based on ribosome depletion were conducted by Genewiz (Leipzig, Germany)