GSM9491454: 57-KO_2; Homo sapiens; RNA-Seq
RNA was extracted with the RNAeasy Mini Kit (Qiagen). RNA samples were quantified, and only samples with concentrations of at least 250 ng/µL were used. Sequencing was performed by the UM AdvancedGenomics Core, with libraries constructed and subsequently subjected to 150 paired end cycles on the NovaSeq-6000 platform (Illumina).