FFPE tissue sections (40 micron scrolls) were deparaffinized, rehydrated, and subjected to gentle enzymatic and mechanical dissociation to release nuclei.  Single-nucleus libraries were prepared using the 10x Genomics Chromium Fixed RNA Profiling platform following manufacturer instructions (Pleasanton, CA, USA). Input targets were approximately 2,000–10,000 nuclei per sample where possible, with a sequencing depth target of ~50,000 reads per nucleus. Libraries were sequenced on Illumina platforms to a combined depth of approximately 700 million reads across experiments. Samples from distinct subjects were multiplexed (pooled and indexed with unique barcodes) and ran as a single run.