LwaCas13a haphazardly targets the human transcriptome
LwaCas13a haphazardly targets the human transcriptome
HEK293T were purchased from ATCC and were grown in DMEM supplemented with 10% FBS. HEK293T cells were co-transfected with plasmids LV-mCherry-T2A-Cas13a and LV-sgRNA-13a-GFP-Neo or LV-sgRNA-13a-GFP-MALAT1 using Polyfect (Qiagen, 301105) following the manufacturer's protocol. LwaCas13a expression was induced by adding doxycycline (Sigma D9891) to the cells growing in media supplemented with TET-free foetal bovine serum (Takara, 631107) for 48 h. Total RNA isolation was done using HP RNA isolation kit (Roche E3101K) following manufacturer's instructions. Illumina stranded mRNA library