Metabarcoding of root fungal community in Cichorium intybus (plant 5)
Experiment1: Plants were grown separately in pots in a glasshouse using a sand:soil mixture. The field-collected soil (2017) was air-dried and sieved, and mixed in a 1:1 ratio with an autoclaved mix of potting soil, coarse sand and pumice (1:2:1). Plants were automatically watered and harvested after 4 month of growth. Roots were cleaned and subsampled for DNA extraction (CTAB extraction). The ITS2 region of the rDNA was amplified by PCR using the primers ITS3 (5-CAHCGATGAAGAACGYRG-3) and ITS4 (5-TCCTSCGCTTATTGATATGC-3) (Tedersoo e al., 2014). The primers included 6nt barcodes for multiplexing. The cleaned PCR products were sent to AGRF (Australia) for Illumina sequencing. The raw data is demultiplexed and includes the primer sequences.