GSM8885662: H1975; Homo sapiens; RNA-Seq
Total RNA was extracted from NSCLC cells using RNeasy Mini Kit (Qiagen, Venlo, Netherlands), and RNA samples were quantified using Qubit 2.0 Fluorometer (ThermoFisher Scientific, Waltham, MA, USA). RNA-seq libraries were prepared using NEBNext rRNA Depletion Kit (NEB, MA, USA) and MGIEasy RNA Directional Library Prep Set (MGI Tech, Shenzhen, Guangdong, China), according to the manufacturer's instructions. The library samples were sent to Genome-Lead Co., Ltd. (Takamatsu, Japan) for RNA-seq analysis (DNBSEQ-G400RS, MGI)) using a 2 × 150-bp paired-end protocol. All sequence reads were converted to FASTQ format using Bcl-2fastq.