RNA-seq of laser-capture microdissected rat hippocampal CA2 following sham-surgery
Total RNA was obtained from laser-capture microdissected hippocampal subregions (CA1, CA2, CA3, dentate gyrus) from adult rats subjected to penetrating traumatic brain injury or control conditions. Due to ultra-low RNA input, samples were lysed directly and cDNA was generated using the SMART-seq v4 Ultra Low Input RNA Kit. Libraries represent bulk transcriptomic profiles of neuron-enriched hippocampal subregions.