GSM8509651: UM67_20; Rhizopus microsporus; MNase-Seq
R. microsporus mycelium was crosslinked with formaldehyde at a final concentration of 1% for 20 minutes in a shaker (80rpm).  For each treatment, we used 20 U of micrococcal nuclease (Thermo Scientific) for 15 minutes at 30 ºC. Digestions were stopped with 100µ of MNase Stop Buffer (80mM EDT and 1.5% SDS). Tubes were centrifuged for 5 minutes at 2700 x g. The supernatant was transferred to a new tube and incubated for 10 min at 95 ºC for reversal crosslinking Truseq library preration kit was used and 30 ng were used for library preparation