DF_a1_13 (CRX1657631; CRA025243)
Genomic DNA was extracted from stool samples using the TIANGEN Soil&Stool Genome DNA Extraction Kit. The DNA was then sheared to ~250 bp fragments using a Bioruptor Pico ultrasonicator. The fragmented DNA underwent end repair, blunt-ending, and phosphorylation, followed by 3' adenylation. Adapters were ligated, and the products were purified. Finally, PCR amplification was performed to construct the sequencing library.