GSM8533667: MuSC_WT_RNA-seq_2; Mus musculus; RNA-Seq
RNA was extracted from 9x10^4 freshly isolated FAC-sorted  MuSCs from control and Taf4asKO mice using  miRNeasy micro Kit (Qiagen). For RNA-seq, RNA was isolated by miRNeasy micro Kit (Qiagen) combined with on-column DNase digestion (RNase-Free DNase Set, Qiagen) to avoid contamination by genomic DNA. RNA and library preparation integrity were verified with LabChip Gx Touch (Perkin Elmer). RNA amounts were normalized and 10ng of total RNA was used as input for SMARTer® Stranded Total RNA-Seq Kit - Pico Input Mammalian (Takara Bio).