GSM8885613: high EPA+DHA, rep2; Magallana gigas; RNA-Seq
Total RNA from tissues was extracted using Trizol; RNA samples were assayed for concentration and purity using a NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific); and RNA sample integrity was measured using an Agilent 2100 Bioanalyzer and 2100 RNA Nano 6000 Assay The integrity of RNA samples was checked using an Agilent 2100 Bioanalyzer and 2100 RNA Nano 6000 Assay Kit (Agilent Technologies). The total RNA that passed the quality control was used as the starting sample, and the TIANSeq mRNA Capture Kit (TIANGEN Biotech) was used to enrich the eukaryotic total RNA with Poly-A structure, and then the library construction was performed with the captured RNA as the library starting sample using the TIANSeq Rapid RNA Library Construction Kit (Illumina platform) (TIANGEN Biotech). TIANGEN Biotech) for library construction. The transcriptome sequencing library was constructed through RNA fragmentation, cDNA one-strand/two-strand synthesis, end repair, A-tail addition, junction ligation and PCR library enrichment.