GSM9015160: Day 1 fibrosis, biological replicate 1; Gallus gallus; RNA-Seq
Wound healing regions were separately isolated from fibrotic (ECZ ) regions. RNA was then  isolated from each of these regions of the post-cataract surgery cultures using the Rneasy mini kit (Qiagen). Sequencing libraries were created using total RNA according to Novogene's protocols. Sequencing was performed on an Illumina NovaSeq 6000 platform and 150bp paired-end reads were generated. Novogene performed the libarary preparation using the following steps: 1) poly-T oligo-attached  magnetic beads were used to purify mRNA, 2)PCR products were purfied on AMPure XP system, 3) The Agilent Bioanalyzer 2100 system  was used to assess library quality. 4) a cBot Cluster Generation System utilizing TruSeq PE Cluster Kit v3-cBot-Hs (Illumia) was used to cluster index-coded samples, and 5) an  Illumina Novaseq platform  was to sequence and create library preparations to generate 150bp paired reads.