Differentiation-stage cochlear-organoid EV miRNA, replicate 2
Extracellular-vesicle RNA was converted into miRNA libraries with the SMARTer smRNA-Seq Kit (Takara Bio). The kit poly-adenylates small RNAs, uses template-switching RT to attach 5/3 Illumina adapters, and adds indexes during PCR. Size-selected libraries (~150 bp) were QC-checked on an Agilent Bioanalyzer, pooled equimolarly, and sequenced as 1  75 bp single-end reads on an Illumina NovaSeq 6000.