WT_RIP
The rRNAs were removed from both the immunoprecipitated RNA and input RNA samples using the NEBNext rRNA Depletion Kit (New England Biolabs, Inc., Massachusetts, USA). The rRNA-depleted RNAs were then used to construct RNA sequencing libraries with the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs, Inc., Massachusetts, USA), following the the manufacturer's instructions.