GSM9555274: Multiome, B-cells, Biological replicate 3, RNA; Homo sapiens; RNA-Seq
Live single CD19+ B sorted into 200ml 100% FBS, washed with ice-cold 1xPBS and then centrifuged at 500 g for 5 min at 4°C. Nuclei were isolated and processed. snRNA/ATAC-seq libraries were generated using the 10x Genomics Single Cell Multiome ATAC + Gene Expression kit, followinf manufacture's instructions.