WGS of Pseudomonas aeruginosa LESB58 arn knockout
genomic DNA extracted using the Qiagen DNeasy Ultraclean Microbial Kit. Next, the sequencing library was prepared using the MinION Rapid Barcoding Kit V14 (SQK-RBK114.24) following the manufacturers protocol and sequenced using MinION Mk1D (Oxford Nanopore Technologies) using a R10.4.1 flow cell. Dorado (v1.1.1, Oxford Nanopore Technologies) was used for base calling. Adapter and quality trimming was performed using dorado trim, using --sequencing-kit SQK-RBK114.24), and files exported as .fastq files.