RNA-seq of black bear muscle
The MGIEasy Fast RNA Library Prep Set is optimized for high-throughput RNA sequencing and can process 10 ng to 1 g of total RNA per sample. The protocol involves: mRNA enrichment using poly-T oligo-attached magnetic beads (for polyA+ selection), Fragmentation of mRNA and synthesis of cDNA, Second strand synthesis by incorporating dUTP in the second strand, which is not amplified during PCR, preserving strand information, End repair, adapter ligation, and PCR amplification to generate the final sequencing-ready library