GSM8676173: 5ECER4G20,fRIPA,Oct4,ChIP; Mus musculus; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis in RIPA buffer, chromatin was sonicated using a E220 focused-ultrasonicator(Covaris). ChIP-seq library was prepared using the KAPA Library Preparation Kit. 1ng of DNA was used as starting material for samples. Library was amplified on the thermocycle and size selected at 50-400bp in length using Agencourt AMPure XP beads from Beckman Coulter. Libraries were validated using the Agilent High Sensitivity DNA kit.