16S rRNA sequencing Delisea pulchra Day 84_Stage III_1
DNA was extracted using the DNeasy PowerSoil Kit. Bacterial communities were characterized by sequencing of amplicons for the 16S rRNA gene. Fragments of approximately 450 base pairs (bp) were amplified by PCR using the primer pair NOCHLFw (S-D-Bact-0341-b-S-17)/799F_rcRv. PCR products were sequenced using Illumina MiSeq technology (2 x 150 bp) at the Ramaciotti Centre for Genomics (UNSW, Sydney, Australia).