RNA-seq mouse stool control day 43 Rep 5
The DNA was extracted from mouse stool using the Qiagen QIAamp PowerFaecal Pro DNA kit. Metagenomic DNA was amplified with indexed primers specific for the V3-V4 region of 16S rRNA (341f/806r) and the libraries were normalized with Invitrogen SequalPrep DNA normalization plates. The pooled libraries were run on a MiSeq platform using Illumina MiSeq v2 500 cycle chemistry in a standard V2 flow cell.