circRNA sequencing in porcine endometrium
Endometrial tissues were collected from pigs during the peri-implantation period and stored at -80C. Total RNA was extracted using TRIzol and assessed for quality. rRNA was depleted, and RNase R treatment was applied to enrich circular RNAs. The RNA was then fragmented, and cDNA was synthesized. After adapter ligation and PCR amplification, the circRNA library was prepared and sequenced on an Illumina platform. Sequencing data were processed to remove low-quality reads, and circRNAs were identified and analyzed for differential expression.