RNAseq for PANFR0195
RNA was extracted from tumor specimens obtained by image-guided needle biopsy or surgical resection, and poly(A)-selected paired-end RNA-seq libraries were prepared using standard Broad Institute protocols. Sequencing was performed on Illumina HiSeq instruments, reads were aligned to the Broad Homo_sapiens_assembly19 (GRCh37) reference with TopHat v1.4.1, and duplicate reads were marked with Picard to generate final BAM files.