GSM8381360: A: SCI plus Atorvastatin Spinal Cord Tissue, Biological Replicate 3; Mus musculus; RNA-Seq
At six weeks post-injury, experimental mice were euthanized with a terminal dose of pentobarbital (60 mg/kg), perfused with ice-cold saline, and spinal cords were isolated. Two uninjured control mice were similarly euthanized and perfused. From each mouse, a 5 mm section centered around the T9-T10 injury site or the corresponding site in uninjured mice was immediately preserved in RNAlater and stored at -80°C. RNA extraction was carried out with Trizol (Invitrogen) following purification with RNeasy Mini Kit (Qiagen), with on-column DNase treatment to eliminate genomic DNA. RNA libraries for RNA-seq were constructed using SMARTer Stranded Total RNA-seq Kit v2 - Pico Input Mammalian (Takara Bio) following the manufacturer's protocol.