GSM9486871: HaCaT cells, siFn14, C.albicans , rep 2; Homo sapiens; RNA-Seq
Total RNA was extracted from keratinocytes and skin tissue using TRIZOL reagent (Life Technologies), according to the manufacturer's instructions. RNA-seq libraries were prepared using the SMARTer Stranded mRNA-Seq Library Prep Kit (Takara Bio) with poly(A) selection, according to the manufacturer's instructions. Libraries were indexed, pooled equimolarly, and sequenced on an Illumina platform .