Foot-and-mouth disease virus from vesicular fluid
Total RNA was extracted using TRIzol LS and RNeasy Mini spin columns with on-column DNase I digestion, then reverse-transcribed into cDNA using the SuperScript IV First-Strand cDNA Synthesis System and the NEBNext Ultra II RNA Second Strand Synthesis Module. The cDNA was sheared to a target size of 400 bp, purified with AMPure XP beads, and prepared into libraries using the GeneRead DNA Library L Core kit with size selection targeting 350550 bp. Libraries were quality-checked, quantified, and sequenced on an Ion Torrent S5 XL instrument with an Ion 530 Chip Kit.