RNA-Seq data from the milk somatic cell transcriptome of Assaf sheep in response to experimental inoculation of an Escherichia coli Lipopolysaccharide
RNA-Seq data from the milk somatic cell transcriptome of Assaf sheep in response to experimental inoculation of an Escherichia coli Lipopolysaccharide
Ten first lactation Assaf ewes were used in this study. Individual milk samples from both mammary glands of each animal were collected at the three time points studied (0h, 6h and 24h) to measure the somatic cell count (SCC) and evaluate the response to the inoculated LPS. Briefly, from each ewe, 50 ml of milk from the inoculated half of the mammary gland was used for RNA extraction. To avoid RNA degradation previously sampling collection, the udder was disinfected with povidone iodine, and nipples were cleaned with RNAseZap (Ambion, Austin, TX, USA). A sterile gauze was used to cover the 50 ml RNAse-free tubes during collection. For RNA extraction, MSCs were pelleted by centrifugation at 650×g for 10 minutes at 4 °C and in the presence of a final concentration of 0.5 mM of EDTA. Then, the MSC pellet was washed twice with 10, 2 mL of PBS (pH 7.2 and 0.5 mM of EDTA) followed by centrifugation at 650×g for 10 min at 4°C. The RNA extraction was performed using 500 μL of TRIzol according to the manufacturer's instructions (Invitrogen, Carlsbad, CA, USA).  Paired-end libraries were prepared using the UltraTM RNA Library Prep Kit (NEBNext®).