GSM8816429: M07e_KO_Replicate_1; Homo sapiens; Bisulfite-Seq
Genomic DNA was extracted from AML cell lines and primary cells using the AllPrep DNA/RNA Kits, Qiagen, following the manufacturer's instructions. DNA purity and concentration were assessed using a Qubit fluorometer. The integrity of the extracted DNA was evaluated using a bioanalyzer. Genomic DNA (500 ng) was digested using the MspI restriction enzyme, which targets CpG-rich regions. The fragmented DNA was then end-repaired, A-tailed, and ligated to methylated adapters using the Zymo-Seq RRBS Library Kit. Bisulfite conversion was performed using the Zymo EZ DNA Methylation Kit according to the manufacturer's protocol. Converted DNA was PCR-amplified, size-selected, and purified before sequencing.