bulk mRNA-sequencing of homo sapiens: IMR-90 primary cell
RNA was extracted from frozen cell pellets using the Zymo DNA/RNA extraction kit (Microprep plus D7005) according to the manufacturers instructions and quantified using a Qubit kit (Thermo Fisher Scientific, USA). Libraries for mRNA analysis were prepared and sequenced using 150 paired-end mode on an Illumina Novaseq platform by Novogene Corp Inc., CA.