CK21_RAW_6_2 (CRX1817635; CRA027213)
TRIzol was used to extract RNA from cells. Oligo(dT) magnetic beads were used to enrich mRNA with polyA structure in total RNA, and ion fragmentation was used to break RNA into fragments of about 300bp in length. Using RNA as a template, 6-base random primers and reverse transcriptase were used to synthesize the first-chain cDNA, and the first-chain cDNA was used as a template for the synthesis of the second-chain cDNA.