GSM8708005: vostok Micro-C repeat 1; Drosophila melanogaster; OTHER
Whole larval brains were dissected from 3rd instar larvae For bulk RNA-seq, the total RNA was extracted in Trizol, following standard protocol and the library was prepared by NEBNext Ultra II Directional RNA Library Prep Kit. For MicroC, the whole brains were fixed in 1% formaldehyde and then DSG and EGS. After MNase digestion, biotin end labeling and proximal ligation, the ligated genomic DNA was extracted by PCI and enriched by streptavidin beads, and finally the libraries were prepared by NEBNext Ultra II DNA Library Prep Kit