GSM9195629: CD45 enriched 6wk VML PCL replicate 1; Mus musculus; RNA-Seq
Quadricep muscles and fibrotic PCL implants were harvested at the 6 wk time point and processed into a single cell suspension. Dead cells were removed with Magnetic-Activated Cell Sorting (MACS) using the Dead Cell Removal kit. MACS with the mouse CD45 MicroBeads was used to separate and collect the CD45+ immune cells (positive fraction) and CD45- stromal cells (negative fraction). Cells were counted in both fractions using a hemocytometer, pooled at in a 1:1 ratio, and processed for 3' scRNAseq (10x Genomics, v3.1 chemistry) The CD45+-enriched samples were processed for 3' scRNAseq (10x Genomics, v3.1 chemistry). Libraries were sequenced using the NovaSeq platform (Illumina) with S2 100 flow cell at a target depth of 100,000 unique reads/cell.