GSM9245900: BVR-O-13 TNO155 rep1; Homo sapiens; RNA-Seq
Flash-frozen tumor tissue samples were pulverized in liquid nitrogen using a mortar and pestle. Tumor tissue DNA and RNA was extracted with the Zymo Research Quick-DNA Microprep Kit (cat. no. D3020) and the Qiagen RNeasy Kit (cat. no. 74134), respectively, according to the manufacturer's instructions. RNA quantification and quality assessment was performed using the NanoDrop Spectrophotometer ND-1000 (software version 3.8.1). Quantified libraries were pooled and sequenced on Illumina platforms and paired-end reads were generated. Hisat2 v2.0.5 were used to build the reference genome and align the paired-end reads. Gene expression was obtained with featureCounts v1.5.0-p3.