16S of rabbits: Sacculus Rotundus
DNA was extracted from 0.2 g using Ultra-Deep Microbiome Prep (Molzym) following manufacturers instruction. Library preparation and sequencing was carried out in Fundacin Parque Cientfico de Madrid under protocols developed and optimized for NGS amplicon sequencing. The V3-V4 16S region was amplified and sequenced using a NextSeq1000/2000 P1 Reagents (600cycles), in a 2 x 300 pair-end sequencing run on a NextSeq 2000 sequencer (Illumina ).