RNAseq-SE_KO-DUX_OE-24h-rep2new
RNA-seq: RNA-seq libraries were generated from mouse embryonic stem cells (mESC) under various conditions, including untreated controls, DUX overexpression (DUX_OE), DUXR70A mutant overexpression, DUX knockdown (KD), SE knockout (KO), and treatments with PlaB, DMSO, RA, and ZSCAN4 overexpression. Each condition was performed in biological duplicates. Total RNA was extracted, and libraries were prepared using polyA enrichment followed by sequencing on an Illumina platform.