GSM8707929: Carboplatin resistant OVCAR-3 (Biological replicate 2); Homo sapiens; RNA-Seq
Cells were lysed using a Trizol reagent (Takara, Japan). RNA was isolated by the chloroform-isopropanol extraction method. All reagents were molecular grade and purchased from Merck. The libraries were prepared from 500 ng of intact RNA using QIAseq Stranded RNA Lib Kit (Qiagen, Cat. No. 180451) according to the manufacturer's protocol and the library QC was done using Agilent D1000 screentape (Agilent, Cat No. 5067-5582) and Qubit DNA HS (Thermo, Q33230).