GSM9487896: NELFA_WT_siNELFA_+DOX_REP1; Homo sapiens; OTHER
Nuclei were extracted following the 'Cell Permeabilization for PRO-seq' protocol from Nascent Transcriptomics Core at Harvard Medical School (https://ntc.hms.harvard.edu/file_url/141). Quick PRO-seq libraries were prepared from 1 million nuclei following the protocol described by Judd et al. (bioRxiv, 2020). Four biotin-11-NTPs were used in the run-on reaction.