Nanopore whole genome sequencing of muscle sample from Vietnamese bos gaurus
Total DNA was extracted from the muscle sample using the Gentra Puregene Tissue kit (QIAGEN, Germany), following the manufacturers instructions. DNA quantity and quality were assessed using a Qubit 4 Fluorometer (Thermo Scientific, USA) and a NanoDrop 1000 Spectrophotometer (Thermo Scientific, Wilmington, DE).Sequencing libraries were prepared using the Ligation Sequencing Kit v10 (SQK-LSK110) from Oxford Nanopore Technologies (ONT, UK) with modifications as described in Hayes et al. 2021 (9), and sequenced on a PromethION device with R9 flow cells (ONT, UK).