RNA-Seq of mus musculus: adult heart ventricle
TRIzol-isolated total RNA samples were prepared for sequencing using the NEBNext Poly(A)mRNA Magnetic Isolation Module (New England BioLabs) to isolate poly(A)+ RNA from total RNA. The eluted RNA was transcribed into a double-stranded cDNA next generation sequencing (NGS) library using the NEBNext Ultra II directional RNA Library Prep Kit for Illumina (New England BioLabs). Quality control and quantification were performed using the TapeStation 4200 system (Agilent Technologies, Santa Clara, CA, USA). Subsequent, libraries were pooled equimolar and sequenced on a NextSeq2000 sequencing system using SBS chemistry (Illumina, San Diego, CA, USA) in single-end mode (72 cycles).