GSM9045829: Stimulated_Slc1a5_3; Mus musculus; RNA-Seq
Oligo(dT) magnetic beads were used to purify and capture the mRNA molecules in the total RNA. The purified mRNA was fragmented and copied into first strand complementary DNA (cDNA) using reverse transcriptase and random primers. A second strand cDNA synthesis step removed the RNA template while incorporating dUTP in place of dTTP in order to preserve strand specificity. Next, double‐stranded cDNA was A‐tailed, then ligated to Illumina anchors bearing T‐overhangs. PCR‐amplification of the library allowed the barcoding of the samples with 10 bp dual indexes and the completion of Illumina sequences for cluster generation.