RNA-seq for chicken 8 weeks hypothalamus
The total RNA of hypothalamus and duodenum were extracted using TRIzol reagent according to the manufacturers recommendations. To eliminate DNA contamination, all samples were treated with RNase-free DNase. The concentration and purity of the RNA sample were determined with a Nano Photometer spectrophotometer, and the RNA integrity number (RIN) of each RNA sample was determined using an Agilent 4150 RNA. cDNA synthesis was performed using a PrimerScript RT Reagent Kit according to the manufacturers instructions. Library construction was performed by Hieff NGS Ultima Dual-mode mRNA Library Prep Kit for MGI.