GSM8707942: RKO-shMETTL3_Input_rep2; Homo sapiens; RIP-Seq
Total and caRNA were isolated from LOVO and RKO cells. One microliter of 1:1,000 diluted m6A and non-m6A spike-in from an EpiMark N6-Methyladenosine Enrichment kit (New England Biolabs, E1610S) was added to 1 μg of purified nonribosomal RNA or mRNA, followed by fragmentation according to previously published protocols14. m6A-IP was performed using an EpiMark N6-Methyladenosine Enrichment kit according to the manufacturer's protocols. RNA library construction was performed using a SMARTer Stranded Total RNA-Seq kit v2 (Takara) according to the manufacturer's protocols. Sequencing was conducted  on an Illumina NovaSeq machine in pair-end mode with 150 base pairs (bp) per read.