GSM9489106: hBMSC_Control_1; Homo sapiens; RNA-Seq
Total RNA was extracted from tissues or cells using standard methods. Libraries were constructed using either the standard NEB protocol or the strand‑specific protocol. Briefly, mRNA was enriched using oligo(dT) beads, fragmented, reverse‑transcribed into cDNA, end‑repaired, A‑tailed, ligated with adapters, size‑selected, and PCR‑amplified.