GSM9631310: 15RP_Pre_260330_invitro3hr; Homo sapiens; RNA-Seq
RNA was harvested using the trizol-chloroform technique followed by the Qiagen Rneasy micro kit. 100ng of total RNA was used to generate RNA-Seq libraries using Illumina stranded mRNA kit (Catalog#: 20040534), following the manufactures protocol.   Libraries prepared with unique dual indexes were pooled at equal molar ratios.