GSM9286018: HiC_ZSCAN4OE_DOX; Mus musculus; Hi-C
Hi-C was performed on FACS-sorted cells for all the experiments. 500,000 cells were fixed with 2% formaldehyde and frozen for at least 12 hrs at -80°C prior to Hi-C experiments. Hi-C was performed using the Arima Hi-C+ kit (Arima Genomics) according to the manufacturer's instructions. DNA was sheared using Covaris. Sequencing libraries were prepared using the Collibri kit (Invitrogen, Cat#A38612024W). For sequencing, PE35 sequencing was carried out using NextSeq 500/550 High Output Kit v2.5 (75 Cycles) (Illumina, Cat# 20024906).