Nanopore DNA-Seq of ESBL-producing K.pneumoniae (ESBLKP) HM28
Approximately 300 ng of DNA (unsheared) was used as the input for Nanopore Native Barcoding Library preparation (SQK-NBD114) according to the manufacturer's instructions. Sequencing was performed on a Minion Flowcell R10.4.1. Basecalling of the pod5 files used Dorado v0.9.0 (SUP basecalling model - dna_r10.4.1_e8.2_400bps_sup@v5.0.0).