GSM8502836: PH 004; Homo sapiens; RNA-Seq
Bone marrow (BM) was collected from the proximal femur during THA or from the distal femur during TKA. During these procedures, bone marrow was extruded and collected into syringes containing heparin (2,500 IU/mL heparin per 10 mL of bone marrow). The bone marrow preparations were further diluted fourfold with phosphate-buffered saline without Ca2+, Mg2+ (PBS). Samples were filtered through a 100-µm cell strainer to remove bone debris and then slowly applied to the density gradient material using a ratio of 1 volume of Biocoll (Biochrom, Berlin, Germany, L6115; ρ