GSM8676865: Embryo 1, lens removed, 6h; Gallus gallus; RNA-Seq
RNA was prepared using TRIzol (Fisher Scientific) followed by purification and removal of genomic DNA using the Qiagen RNeasy mini kit with on-column DNase I digestion (Qiagen), according to the manufacturers' instructions. Libraries were prepared using the Illumina TruSeq Stranded mRNA kit with unique dual indices and sequenced on an Illumina NextSeq 500 with v2.5 chemistry and 75 bp paired end reads.