GSM9096974: D163_24HRS_cFBG; Homo sapiens; RNA-Seq
Macrophages were stimulated as described above and RNA was isolated after stimulation using RNeasy Micro Kit (Qiagen) according to the manufacturer's instructions. Sample QC and sequencing were performed by the Oxford Genomics Centre. cDNA was generated from total RNA using the Lexogen 3' QuantSeq FWD technology, i.e. using first oligodT priming followed by random priming. cDNA libraries were then amplified to enable single-end sequencing with Illumina. The prepared libraries were QC'ed and multiplexed before sequencing over two units of a flow cell, resulting in a total of 8 lanes per sample.