RNA-Seq_ CTR_8h_sample_2
Messenger RNA was purified from total RNA using poly-T oligo-attachedmagnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the secondstrand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. Thelibrary was checked with Qubit and real-time PCR for quantificationandbioanalyzer for size distribution detection.