RNA-seq of Staphylococcus aureus isolates from chronic rhinosinusitis patients (CSS) against healthy individuals
RNA-seq of Staphylococcus aureus isolates from chronic rhinosinusitis patients (CSS) against healthy individuals
S. aureus isolates from chronic rhinosinusitis patients (CSS) and healthy individuals (MIN) were cultured overnight.Cultures were diluted in TSB broth to an optical density (OD600) of 0.05 the following day, and samples were collected at 3- and 6-hours post-dilution.  RNA from harvested samples were isolated using Zymo Research Quick-RNA Fungal/Bacterial Miniprep Kit standard protocol and rRNA was depleted using Invitrogen MICROBExpressâ„¢ Bacterial mRNA Enrichment Kit kit. Nucleic acid library was prepared using standard Illumina RNA-seq protocol that uses random hexamer primers that for cDNA synthesis.