GSM9487524: 3cm spike peduncle, 30DPA, biol rep 3; Triticum turgidum subsp. durum; RNA-Seq
Total RNA was extracted from 100 mg fresh weight material; the Direct-Zol RNA Miniprep Kits Zymo Research) was used for leaf, root, meristem and ovary samples, while the RNeasy Plant Mini Kit (Qiagen) was used for seed samples. In both protocols genomic DNA contamination was removed using the on-column DNase treatment Firstly, ribosomal RNA was removed from total RNA, followed by ethanol precipitation. After fragmentation, the first strand cDNA was synthesized using random hexamer primers. During the second strand cDNA synthesis, dUTPs were replaced with dTTPs in the reaction buffer. The directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification