Illumina sequencing of Bauhinia purpurea
Prepared by constructing Paired-end libraries, meaning that sequencing primer binding sites are added to the splices at both ends when constructing DNA libraries to be tested, after the first round of sequencing is completed, the template strand from the first round of sequencing is removed, and a Paired-End Module (Paired-End Module) is used to direct the regeneration and amplification of the complementary strand in its original position to achieve the amount of template used for the second round of sequencing, and the The second round of complementary strand synthesis sequencing.