GSM8816761: Wheat Straw , mid of the exponentiel phase, rep1; Ruminiclostridium cellulolyticum; RNA-Seq
After thawing of bacterial pellets, cells were treated with lysozyme (1g.L-1) in TE buffer and incubated for 20 minutes at 37°C. After lysis, total RNA were extracted using the "Maxwell® 16 miRNA Tissue Kit" (Promega) and treated with the TURBO DNA-free™ kit (Invitrogen) following the supplier protocols. After quality control using TapeStation microfluidic electrophoresis with RNA ScreenTape (Agilent Technologies), the samples were stored at -80°C. Illumina RNA-seq libraries were prepared using the Zymo-Seq RiboFree® Total RNA Library Kit (ZYMO RESEARCH) using 250 ng of total RNA and following manufacturer's protocols. After quality control using TapeStation microfluidic electrophoresis with High Sensitivity D1000 ScreenTape (Agilent Technologies) and DNA quantification using a Qubit fluorometer (Thermo Fisher Scientific), the sequencing was performed using an Illumina NextSeq 500 sequencer (TGML sequencing platform, Marseille, France) and following manufacturer's protocols.