GSM8564846: E17-5_LSK_WT_ADT; Mus musculus; OTHER
E17.5 fetal livers were harvested and genotyped before 10 embryos/genotype were pooled. Cells were bead enriched for c-Kit+ cells before surface staining. 1-2 million cells/50µl were stained with fluorophore-conjugated and barcoded Totalseq-B (Biolegend) for 30 minutes at 4°C. 20,000 LSK cells were double sorted (yield, then purity setting) on a BD FACSAriaII into PBS with 0.04% BSA. Cells were counted and resuspended in PBS with 0.04% BSA at a concentration of 1,200 cells/µl. 10,000 cells were loaded into a 10x Chromium Controller. 10x Genomics Chromium Next GEM Single Cell 3' v3.1 (Dual Index) Kit with Feature Barcoding (PN-1000269), Dual Index NT Set A, and Dual Index TT Set A were used to further process samples according to manufacturer's instructions. 10x Genomics Chromium Next GEM Single Cell 3' v3.1 (Dual Index) Kit with Feature Barcoding (PN-1000269), Dual Index NT Set A, and Dual Index TT Set A were used to further process samples according to manufacturer's instructions.