18S rRNA amplicon sequencing of South Pacific microbes from GO-SHIP P18 line
RNA was extracted using the NucleoMag RNA kit (Macherey-Nagel). The V9 region of 18S rRNA was amplified with the primers 1389F (5-TTGTACACACCGCCC-3) and 1510R (5-CCTTCYGCAGGTTCACCTAC-3).