GSM8381341: totalRNA_sonkd_rep1; Mus musculus; RNA-Seq
24 hours post-transfection, RNA extraction was performed with TRIzol reagent following instructions of the manufacturer. RNA amount and quality was assessed with Qubit and BioAnalizer. Rt-qPCR was performed to validate Son KD at the mRNA level. For total RNA-seq: RNA samples were delivered to sequencing company in dry ice. Depletion of rRNA was performed in sequencing location prior to library preparation. For Nanopore cDNA sequencing: library preparation was performed following the instruction provided by the manufacturer (Flow-cell:FLO-MIN106D ;Protocol: cDNA).