Human gut metagenome from MIAB sample 99, Zymo_Three_Peaks extraction, replicate 2, Oxford Nanopore
Human gut metagenomic DNA extracted using Three-Peaks method (magnetic bead and manual extraction), technical replicate 2. Library prepared using Oxford Nanopore Ligation Sequencing Kit (SQK-LSK110) with NEBNext Companion Module and Agencourt AMPure XP beads. Targeted 100-200 fmol DNA in 47 L per sample. Loaded onto PromethION R9.4.1 flow cell with 72-hour run length. Basecalling performed using Guppy v4.0.11 with minimum quality score threshold of 7.