FACHB-3719 (CRX1629712; CRA024790)
The DNA sample was used as input material for the DNA library preparations. Sequencing library was generated and index codes were added to each sample.Briefly, genomic DNA sample was fragmented by sonication to a size of 350 bp. Then DNA fragments were end-polished, A-tailed, and ligated with the full-length adapter for Illumina sequencing, followed by further PCR amplification. After PCR products were purified. Subsequently, library quality was assessed and quantified by qPCR. The qualified libraries were pooled and sequenced on Illumina platforms with PE150 strategy, according to effective library concentration and data amount required.