GSM9630827: WT Bmem (4wk)-rep2; Mus musculus; RNA-Seq
Sorted cells were lysed in TRIzol and total RNA isolated using the miRNeasy Micro Kit (#217084, Qiagen), following the manufacturer's instructions. The isolated RNA was further processed using the Monarch RNA Cleanup kit 10ug (New England BioLabs Inc #T2050L). Libraries were prepared from 0.5ng of total RNA using the Takara Bio SMART-Seq v4 PLUS Kit (Takara Bio R400753) following the manufacturer's instructions.