GSM8447209: 28 Planktonic LB 0% Rep3; Escherichia coli; RNA-Seq
RNA was harvested using RNAprotect (Qiagen) and Rneasy Plus kit (Qiagen). 1 µg or greater was sent for sequencing. rRNA removal by using Ribo-Zero Plus rRNA Depletion Kit (Illumina) rRNA depleted samples were quantified and used for library preparation using the KAPA mRNA HyperPrep Kits (Roche). Following the library preparation, the final concentration of all of the libraries was measured using the Qubit dsDNA HS Assay Kit (Life Technologies), and the average library size was determined using the Agilent 2100 Bioanalyzer (Agilent Technologies)