GSM9453763: shControl_Cluster_Rep2; Homo sapiens; RNA-Seq
Samples were washed with PBS prior to lysis in RLT buffer, homogenization using QIAShredder columns (Qiagen cat. 79656), and RNA extracted with the RNeasy kit (Qiagen cat. 74016) using a 15 minute DNAse treatment ( Qiagen cat. 79254). RNA quality was confirmed by Agilent Bioanalyzer, and TruSeq PolyA enriched libraries were generated (Illumina).Samples were sequenced on an Illumina HiSeq 4000 to generate a minimum of 25M paired-end reads.