16S rRNA amplicon of environmental community: reference water column sample
Amplicons are produced with KAPA HiFi Hotstart polymerase. DNA amplicons are then pooled equimolar and an Illumina-compatibel library was made with "NEBNext Ultra II DNA Library Prep Kit for Illumina" followed by sequencing on NextSeq 2000 in 2 x 300 bp paired-end read mode