GSM8045884: ChIPseq_DMC1_KEInput_sticklebackTestesPool_Freshwater; Gasterosteus aculeatus; ChIP-Seq
Tissue lysate was prepared from frozen tissue following the protocols described in Smagulova et al (2011) Nature 472:375-8 and Khil et al (2012) Genome Research 22:957-65. The ChIP pulldown of DMC1-bound single stranded DNA was performed with "kinetic enrichment" following the protocols described in Smagulova et al (2011) Nature 472:375-8 and Khil et al (2012) Genome Research 22:957-65 and was followed sequentially by a ChIP pull down H3K4me3 on the same tissue lysate.