Element AVITI Standard Insert Sequencing of the GIAB HG002
Sequencing was performed on the Genome In A Bottle HG002 (NIST RM DNA). The standard insert library (400 bp) was prepared using 1ug gDNA as input into Kapa HyperPrep (Kit code: KK8504) with Kapa UDI (cat no:08861919702). Mechanical shearing of gDNA was performed using a Covaris ultra-sonicator to achieve the desired insert size of ~400 bp. Size selection was performed with double-sided SPRI bead clean-up with SPRI ratios of 0.46X/0.62X. PCR-free prep library treated with USER enzyme and circularized with Adept Rapid PCR-free workflow using the Element Library Compatibility Kit v1.1 (cat no:830-00007). The libraries were loaded onto two individual high-output flowcells. Paired-end 2x150bp reads were sequenced using in-development sequencing reagents and protocol on the Element AVITI platform with the AOS v2.3.0 software.