GSM9046426: Control 4; Homo sapiens; RNA-Seq
Total RNA was extracted from the tumours of the orthotopic LNCaP xenograft model using the AllPrep DNA/ RNA/miRNA Universal Kit (# 80224, Qiagen). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library.