GSM9629656: xSCC, tumor xenograft, replicate 3; Homo sapiens; RNA-Seq
Total RNA was extracted from biopsies of HD skin, huSCC, ES and xSCC using the Monarch Total RNA Miniprep Kit (NEB, Cat# TS2010). RNA quantity and integrity were assessed with Qubit 2.0 (Thermo Fisher Scientific) and Agilent 2100 Bioanalyzer (RNA 6000 Pico Kit, Cat# 5067-1513). Libraries were prepared with the NEBNext® Poly(A) mRNA Magnetic Isolation Module, Ultra™ II Directional RNA kit, and Multiplex Oligos for Illumina (New England Biolabs), quantified with Qubit 2.0 assay and assessed on the 2100 Bioanalyzer (High Sensitivity DNA Kit, Cat.# 5067-4626). Sample-specific libraries were pooled equimolarly