D3
Genomic DNA was extracted from pure overnight cultures grown in Todd Hewitt broth using the Monarch Genomic DNA Purification Kit (New England Biolabs, #T3010L). DNA was normalised and sheared to ~350 bp fragments by sonication, followed by end-repair, dA-tailing, and ligation of Illumina-compatible adapters.Uniquely indexed libraries were pooled and sequenced on a NovaSeq 6000 (S4 flow cell) 2  150 nt paired-end data.