GSM8502130: OV744HG; Homo sapiens; RNA-Seq
CD45+ and CD45- live cells were sorted from primary ovarian tumour resections (N=3) and mixed in a 1:1 ratio to ensure enough enrichment from both immune and tumour proportions. Samples were prepared using Chromium Single Cell 5' v2 Reagent Kit (Catalog no. PN-1000263, 10x Genomics) according to the manufacturer's instructions. Library size was determined using Agilent High Sensitivity DNA Kit (5067-4626, Agilent Technologies). Samples were pooled and quantified using QubitTM dsDNA HS Assay Kit (Q32851, Qubit) on the QubitTM 2.0 Fluorometer. Pools were sequenced with an S2 flow cell on NovaSEq 6000 platform (Illumina) to a targeted depth of 50,000 reads per cell.