The transcriptomic data of V. cholerae strains N16961 and  the VC1123 mutant  in LB medium
rRNA was depleted using the RiboCop rRNA Depletion Kit for Mixed Bacterial Samples (Lexogen, USA). The resulting mRNA was fragmented (~200 bp) and reverse-transcribed into strand-specific cDNA using random primers with dUTP incorporated during second-strand synthesis. After end repair, A-tailing, and adapter ligation, the dUTP-containing second strand was removed by UNG treatment. Libraries were prepared using the Illumina Stranded mRNA Prep, Ligation kit (Illumina, San Diego, CA), and paired-end sequencing was performed on the Illumina NovaSeq X Plus platform.