RNAseq analysis of tumours from GEMMS animals to determine the effect of different aPDL1 combinations on lung tumours in situ
RNAseq analysis of tumours from GEMMS animals to determine the effect of different aPDL1 combinations on lung tumours in situ
Samples were collected from GEMMs lung lesions and assessed by MRI quantification of combined lung tumour lesions per animal before & after treatment   RNA was extracted using the RNeasy Mini Kit (Qiagen; #74104). RNA concentration was determined by Qubit Flex Fluorometer (Invitrogen), RNA purity was determined using a NanoDrop Eight (Thermo Scientific) and RNA integrity was measured using a 4200 Tapestation (Agilent) Libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs; E7760L) or NEBNext® Ultra™ II RNA Library Prep Kit (New England BioLabs; E7770L) as per manufactuer's guidelines. Ribosomal RNA was removed using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England BioLabs; E7490L) .