DAP-seq of Mus musculus HT22 cell
After DAP DNA was extracted, the enriched DNAs were fragmented into short fragments by ultrasonic. Next, the DNA fragments were end repaired, 3A added, and ligated to Illumina sequencing adapters. DNA fragments with proper size (usually 100-305bp, including adapter sequence) were selected for PCR amplified. Finally we got qualified library for sequencing.