GSM8045565: glutamatergic neurons, heterozygous, clone 42.13, rep2; Homo sapiens; RNA-Seq
TRIzol reagent was used to extract, chloroform for phase separation of, and ethanol to clean total RNA. Total RNA was used for library preparation. RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina following manufacturer's instructions (NEB, Ipswich, MA, USA).