GSM8381008: FLS stimulated by CCN3 (NOV) 3; Homo sapiens; RNA-Seq
hFLS RNA was purified using TRIzol Reagent and RNA extraction kit (NucleoSpin®) according to the manufacturere's protocol Libraries were generated from 1 µg of high-quality RNA using NEBNext Ultra II Directional RNA Library Prep Kit  .Data of 150 bp× 2 paired-end of 4-6G bases per sample (3 samples for each condition) was trimmed by fastp and mapped by alignment to the mouse mm10 reference genome using STAR. The reads were quantified using RSEM and the raw count data were subjected to Subio Platorm Plug-ins software .