GSM8413283: Zebrafish, usp39, 3; Danio rerio; RNA-Seq
Samples were homogenized in 200 µl RNAzol® RT (Sigma-Aldrich) in gentleMACS™ M Tubes using gentleMACS™ Octo Dissociator with Heaters (Miltenyi Biotec), program RNA_02.01. RNA was extracted following the RNAzol® RT extraction protocol. Ribosomal RNA was depleted from 1.0 µg total RNA using RiboCop rRNA Depletion Kit for Human/Mouse/Rat (Lexogen) following the manufacturer's instructions. Sequencing libraries were generated using the CORALL RNA-Seq kits (Lexogen) following the manufacturer's instructions.