GSM8042745: Healthy kidney tissue, KI_N1; Homo sapiens; RNA-Seq
RNA from tissue specimens was extracted from fresh frozen tissues that were removed during surgery. Total RNA was extracted using a RNeasy mini kit (Qiagen, Germany), and was assessed using Bioanalyzer (Agilent, US), Nanodrop (Wilmington, DE) to ensure high quality and purity (RNA integrity number >7, OD260/280>1.8). mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. Sequencing libraries were constructed using NEBNext Ultra RNA Library Prep Kit for Illumina (San Diego, CA).