GSM8381925: MCF10A cells, wildtype, 2D culture, biol rep 1, H2AK119ub1; Homo sapiens; ChIP-Seq
1 million cells were fixed with 1% formaldehyde for 8 minutes, and sonicated for 8 cycles on a Bioruptor Pico (Diagenode). ChIP-seq against H2AK119ub1 (D27C4, Cell Signaling Technology) was performed with the iDeal ChIP-seq kit for histones (Diagenode) as per the manufacturer's protocol. Libraries were prepared using the TruSeq ChIP Sample Prep kit (Illumina) using 10ng of DNA as starting material for inputs and IP samples.