GSM9337908: EVMu-1; Rattus norvegicus; RNA-Seq
Total RNA was extracted from primary dorsal root ganglion (DRG) neurons using TRIzol® Reagent according to the manufacturer's instructions with minor modifications. Poly(A)+ mRNA was enriched from total RNA using Oligo(dT) magnetic beads, then randomly fragmented. First-strand cDNA was synthesized with random hexamer primers, followed by second-strand cDNA synthesis. The resulting cDNA underwent end repair, A-tailing, adapter ligation, size selection, PCR amplification, and purification to generate the final sequencing library.