GSM9491423: E17.5 Prospermatogonia WT 3; Mus musculus; RNA-Seq
RNA was extracted from snap frozen cell pellets using TRIzol reagent followed by chloform phase separation and isopropanol percipitation. To eliminate carry-over genomic DNA, total RNA was treated with Qiagen DNaseI followed by clean up using RNAClean XP beads. Ribosomal RNA-depleted total RNA libraries were generated using the SMART-seq Total RNA Pico Input Kit with UMIs and ZapR Mammalian from Takara Bio. 5ng of total RNA was used as input.