GSM9630071: Testic cells,rep5; Homo sapiens; RNA-Seq
Human testis samples were minced with sterilized scissors after washing three times with PBS.Then were digested in 1 mg/ml collagenase type IV at 37C for 15 min. During the process of digestion, the tissues were pipetted up and down once in every seven minutes.The digestion process was stopped with DMEM (containing 10% FBS).The cell suspension was filtered through 40-mm nylon mesh. After centrifugation, the cells were suspended in DMEM (containing 10% FBS) for further sample selection. The cell suspension was loaded into Chromium microfluidic chips with 3' v3.1 chemistry and barcoded with a 10× Chromium Controller (10X Genomics). RNA from the barcoded cells was subsequently reverse-transcribed and sequencing libraries constructed with reagents from a Chromium Single Cell 3' v3.1 reagent kit (10X Genomics) according to the manufacturer's instructions. Sequencing was performed with Illumina  NovaSeq 6000  according to the manufacturer's instructions (Illumina).