GSM8987650: A205, aged female; Mus musculus; RNA-Seq
After specified timepoints, animals were euthanized. Then leg skin was dissected with tweezers and muscles exposed and under real time magnification using an illuminated magnifier (Omano, China) muscles were dissected individually, cutting first the distal tendon, and gently removing the entire muscle using dissecting scissors (Roboz RS-5840). After the proximal tendon was severed, the muscle was put in contact with a gauze under magnification to eliminate remaining blood, fat, and if needed also remaining tendon using scissors. Muscle was then weighed using an analytical balance (Sartorius Entris, Germany). Mass determination was performed on the tibialis anterior (TA), extensor digitorum longus (EDL), and soleus muscles. RNA was sent to GENEWIZ for extraction, library preparation of poly A enrichment, and sequencing on an Illumina Novaseq 6000