GSM8849324: HEK293T cells, heat shock treatment (43°C for 1 hour), rep2; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol reagent (Invitrogen) following the manufacturer's protocol. After rRNA was removed first, RNA sample was fragmented, end repair, adapter ligation, reverse-transcribed and libraries were amplified with 12 PCR cycles.