RNA-Seq of mussel digestive gland
Enriching eukaryotic mRNA with magnetic beads containing Oligo (dT); Add Fragmentation Buffer to randomly interrupt mRNA; Using mRNA as a template, synthesize the first cDNA strand and second strand, and purify the cDNA; Purified double stranded cDNA is subjected to end repair, followed by adding an A-tail and connecting sequencing adapters, followed by fragment size selection using AMPure XP beads; Finally, the cDNA library was obtained through PCR enrichment