Whole Genome Sequencing of Escherichia coli: DE502 at 0mins
Samples were collected as flash-frozen pellets. After thawing                 samples were extracted using the Monarch Genomic DNA Purification Kit (NEB #T3010)                 protocol for the manufacturers protocol for gram-negative bacteria. DNA was                 quantified by Qubit and subsequently tagmented using a Nextera XT Kit (Illumina).                 Following library prep, samples were subject to quality control by tapestation                 (Agilent 2200 TapeStation D1000 HS ScreenTape). Finally, libraries were pooled with                 equal stoichiometry and sequenced on a NextSeq 500 using a high output, 75 cycle kit                 (Illumina # 20024906). All library prep and sequencing in this section was performed                 by staff at the Biopolymers Facility at Harvard Medical School.