GSM8042168: OTX2-dTAG_DE_ATAC_dTAG_Rep1; Mus musculus; ATAC-seq
ATACseq was performed on 50,000 DE cells per sample using the Nextera DNA library preparation kit (Illumina FC121103) according to the manufacturer's instructions Libraries were constructed using the Nextera DNA library preparation kit (Illumina FC121103) and NEBNext High-Fidelity 2X PCR Master Mix (NEB M0541) according to the manufacturer's instructions. Cycles for PCR amplification were determined individually for each sample by qPCR Libraries were size selected (0.55–1.5x) using SPRI beads (Beckman Coulter)