GSM8817752: HCC_SnRNAseq2; Mus musculus; RNA-Seq
Livers were isolated, and tumor tissues were dissected. For single-nucleus isolation, 50 mg tumor tissue pieces were frozen in dry ice and stored in liquid nitrogen until nuclei extraction. Single-nucleus suspensions were prepared and processed using the Chromium Next GEM Single Cell 3′ Reagent Kit v3.1 (Dual Index) (10X Genomics, 1000268) to generate single-nucleus RNA-seq libraries. cDNA amplification and library preparation followed manufacturer protocols.