GSM8988064: scRNAseq-Ovary-H0; Mus musculus; RNA-Seq
Ovaries were collected at 0 (H0), 1 (H1), and 6 hours (H6) post-hCG injection for single-cell RNA-seq. Mouse ovaries were collected at H0, H1 and H6 for single-cell RNA-seq, which was conducted by Yingzi Gene (Wuhan, China). Initially, the cell number and viability were assessed, followed by loading cells onto the 10X Chromium Single Cell Platform (10X Genomics) at a concentration of 1,000 cells per μl. The protocol included the generation of gel beads in emulsion (GEMs), barcoding, GEM-RT clean-up, cDNA amplification, and library construction per the manufacturer's instructions. Library quantification was performed using Qubit before pooling, and the final library pool was sequenced on the Illumina Nova6000 platform using 150-base-pair paired-end reads.