Rumen metagenome of dairy goats
The total microbial DNA of rumen contents was extracted using the TIANamp stool DNA kit. The extracted DNA was eluted in 60 L of elution buffer and the quality and concentration of that were determined through 1.0% agarose gel electrophoresis and NanoDrop 2000 spectrophotometer, respectively. The DNA was randomly fragmented by sonication for paired-end library construction. The qualified metagenome libraries were then subjected to high-throughput sequencing using Illumina NovaSeq6000  PE150 platform.