GSM9047404: Colon resident CD8+ T cells, HIV-Negative, Rep8; Homo sapiens; RNA-Seq
RNA was extracted from organoids using TRIzol and Ambion PureLink RNA Mini Kit (Thermo Fisher Scientific, 12-183-018 A) according to the manufacturer's instructions. For RNA-seq library preparation, NEBNext Ultra II Directional RNA preparation with poly(A) selection is used to selectively capture mRNA from total RNA and barcoded libraries were generated. 2 ×40 paired-end sequencing was performed on an Illumina NextSeq 500.