16S-Seq of mus musculus
After intervention with antibiotics and LPS, mice received fecal transplantation (FMT; twice weekly gavage for 6 weeks). For the FMT treatment, three random samples from ARDS patients and healthy individuals were mixed equally by weight, and 1 g of the mixed feces was suspended in 5 mL PBS. The first gavage volume was 100 μL, and subsequent gavage volumes were 200 μL. The Control group served as the control samples at week 9 (before the start of FMT). Fecal samples were collected from mice at ages 9 weeks (before the start of FMT) and 14 weeks, with mice weighed before tissue collection. Feces were stored at -80C.