GSM8536766: Neuron derived from CTRL2-iPSC,Div35; Homo sapiens; RNA-Seq
Total RNA was extracted from DIV35 neurons using TRIZOL Reagent (Thermo Fisher Scientific), according to the manufacturer's protocol. The extracted RNA was further purified and enriched using NucleoSpin RNA Clean-up XS (Macherey-Nagel) according to the manufacturer's protocol. Each RNA sample was evaluated for purity and concentration using BioDrop µLite (BioDrop), and the A260∶A280 ratio was 1.8~2.2, A260∶A230 ratio was 1.8 or higher, and the concentration was 50ng/µl. Sequences of each sample were obtained after library preparation by the poly-A selection method using NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) and NEBNext® UltraTMII Directional RNA Library Prep Kit (New England Biolabs) following manufacturer`s protocol.