GSM9595552: Immortalized hepatocyte cell line DeltaCN60; Mus musculus; RNA-Seq
Cells were cultured until they reached about 80% confluence before being lysed with guanidine thiocyanate for total RNA extraction using the cesium chloride cushion method. All library preparation and sequencing were performed in a single batch, so no batch effects were present, and no batch correction was applied. Paired-end RNA sequencing was carried out on an Illumina MiSeq platform using a MiSeq Reagent Kit v2 (300 cycles).