RNA-seq of WT Plasmodium berghei
Total RNA was extracted from activated gametocytes and schizonts of P. berghei WT-GFP and 778 ark1PTD parasites (three biological replicates each) using the RNeasy Kit (Qiagen), vacuum-779 concentrated, and transported in RNA-stable tubes (Biomatrica). Strand-specific mRNA 780 libraries were prepared with the TruSeq Stranded mRNA Sample Prep Kit (Illumina) and 781 sequenced on an Illumina HiSeq 4000 platform (paired-end, 150 bp reads).