Rodent metagenome Canaa dos Carajas sample 6
Biological samples from wild rodents were collected during eco-epidemiological expeditions in the municipalities of Cana dos Carajs and Curionpolis, located in the State of Par, between 2017 and 2019. For RNA extraction, fragments of viscera (brain, lung, kidney, spleen, and heart) were used, with pools created for each rodent species captured. The extraction products were standardized to a concentration of 0.2 ng/L, fragmented, and labeled with adapter sequences. The resulting genomic libraries were quantified and sequenced using the paired-end method (2 x 150) on the NextSeq 500 System platform. The sequencing files were demultiplexed using bcl2fastq v.2.20 and subjected to quality control using Fastp v.0.23.4, configured to remove adapter sequences and reads with a Phred base quality < 20 and a length shorter than 50 nt. Contigs were assembled de novo using MEGAHIT v.1.2.9 in its default configuration (k-mers of 21, 29, 39, 59, 79, 99, 119, and 141 nt), identified using DIAMOND v.2.1.9.163 (Blastx mode, e-value of 0.00001), and manually inspected using Geneious v.11.1.5.