GSM9594169: CeA, G18, Male, Dependent, IP; Mus musculus; RNA-Seq
Translating Ribosome Affinity Purification (TRAP) was carried out on nucleus accumbens brain punches to isolate astrocyte translating RNA. An aliquot was saved prior to the TRAP procedure to serve as input samples which represents the bulk-tissue RNA. RNA libraries were generated with Takara's SMART-Seq PLUS kit according to manufacturers protocol.