RNA-seq of Leporinus friderici: adult males/females brain
Tissue samples from the hypophysis and gills were collected from adult male and female L. friderici, whereas testes were collected from adult males only. Samples were preserved in RNAlater (Applied Biosystems), kept refrigerated overnight at 4 C, and subsequently stored at 20 C until RNA extraction. Total RNA was extracted separately from each tissue using TRIzol reagent (Invitrogen, CA, USA), following the manufacturers instructions.