GSM9011428: BALB/c 3 ATAC-seq; Mus musculus; ATAC-seq
A FACSAria III cell sorter (BD) was used for live cell sorting. 50,000 cells were used to extract the nuclei. The nuclei were treated with Tn5 transposase (Illumina) to tag and fragmentize the accessible chromatin. The fragmentized DNA was purified using a QIAGEN MinElute kit (QIAGEN) and amplified by 10−12 PCR cycles, based on the amplification curve. The libraries were sequenced for 100 cycles.