WGBS of Mus musculus HT22 cell
After genomic DNAs were extracted from the samples, DNA concentration and integrity were detected by NanoDrop spectrophotometer and Agarose Gel Electrophoresis respectively. The DNA libraries for Enzymatic Methylation sequencing were prepared as described previously. Briefly, genomic DNAs were fragmented into 100-300bp by Sonication (Covaris) and purified with MiniElute PCR Purification KitQIAGEN. Purified DNA is enzymatically converted using a EpiArtDNA Enzymatic Methylation Kit. The fragmented DNAs were end repaired and a single A nucleotide was added to the 3 end of the blunt fragments. Then the genomic fragments were ligated to methylated sequencing adapters. Finally, the converted DNA fragments were PCR amplified and sequenced using Illumina NovaSeq X Plus.