GSM8817239: Placenta, Deep placenta, preeclampsia preterm parturitions, 5; Homo sapiens; RNA-Seq
Placental tissues were obtained during Cesarean section, and the maternal-fetal (MF) and deep-placenta (PL) layers were freshly isolated. Samples underwent general cell processing steps, including viability and concentration assessment using a Countess II FL Automated Cell Counter (Thermo). RNA-seq libraries were prepared using the Chromium Single Cell 3′ Library Kit (10X Genomics) according to the manufacturer's instructions. Samples containing more than 10,000 cells per condition were loaded onto the Chromium Controller for single-cell partitioning and barcoding. The resulting cDNA libraries were amplified and sequenced on an Illumina HiSeq-X sequencer, generating an average of 11,117.7 ± 3,057.9 single-cell transcriptomes per sample.