SLE and atherosclerosis CD8+ T-cell RNAsequencing
SLE and atherosclerosis CD8+ T-cell RNAsequencing
Venipuncture. Patients were scanned for carotid and femoral atherosclerotic plaques by vascular ultrasound. Blood was collected and CD8+ T-cells were isolated for sequencing/ Qiagen PAXgene Blood storage to isolate PBMCs. CD8+ T-cells were isolated using FACSAria flow cytometry. RNA was isolated using Picpure RNA isolation kit. Sample concentrations and purity were determined using Agilentâ€™s 2200 Tapestation Sequencing, quality control analysis, genome alignment and quantification was performed by UCL Genomics (London, UK)