GSM9487274: 14 DPI SSD Diet Hp Infection 3; Mus musculus; RNA-Seq
RNA was isolated utilizing the miRNAeasy Mini Kit (Qiagen #217604) in accordance with the manufacturer's protocol. Intestinal tissue samples were homogenized in Qiazol lysis reagent using a gentleMACS Dissociator (Miltenyi Biotec, Germany) and subsequently processed through an RNAeasy spin column (from Qiagen kit) with on-column DNase treatment. The RNA concentration and purity were then assessed using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, DE, USA). standard Illumina