GSM9630502: HeLa_siEPHB2_si2_rep1; Homo sapiens; RNA-Seq
Total RNA was isolated from sample using RNeasy mini kit (Qiagen, Germany). Paired-end libraries were synthesized using the TruSeq® RNA Sample Preparation Kit (Illumina, USA) following the manufacturer's instructions. Briefly, poly(A)-containing mRNA was purified using poly-T oligo-attached magnetic beads. Purified libraries were quantified using Qubit® 2.0 Fluorometer and validated using an Agilent 2100 Bioanalyzer to confirm insert size and molar concentration. Cluster generation was performed by cBot with the library diluted to 10 pM.