GSM9632140: Scrambled control, rep2; Homo sapiens; RNA-Seq
RNA was extracted from cell pellets washed with PBS using Directzol RNA MiniPrep (Zymo Research Corporation; Fisher Cat# 50-444-628). RNA yield and purity was tested using the Qubit 4 Fluorometer prior to analysis. PolyA RNA isolation and library preparation was performed with the Watchmaker mRNA Library Prep Kit (Watchmaker Genomics: 7BK0001) with Elevate Long UDI Adapter Kit Set A (Element Biosciences, Cat: 830-00010). Libraries were sequenced with the Element Biosciences AVITI Sequencing platform using the AVITI 2x75 High Output Cloudbreak Kit (Element Biosciences, Cat: 860-00004).