GSM9452930: SNL_TO_sgNTCi; Mus musculus; RNA-Seq
Total RNA was extracted from SNL tumour organoids after digestion to single cell suspension using a RNeasy mini kit (Qiagen Cat. No. 74104) cDNA libraries were prepared using the Watchmaker mRNA library prep kit (Watchmaker Genomics 7BK0001). Libraries were sequenced on a NextSeq 1000 with a read length of 100 aiming for at least 30M paired reads per sample.