GSM8535331: RNAseq_oocyte_DQ_1; Mus musculus; RNA-Seq
For single oocyte analysis and data processing, 3 oocytes was extracted using a TRIzol reagent following a standard protocol. The RNA integrity was checked on Agilent 2100 BioAnalyzer to conduct quality control (Agilent Technologies). For RNA-seq data processing, the high-throughput RNA-Seq were performed with the NovaSeq 6000 platform to a depth of 1.5×107 reads.