16S rRNA skin microbiota of Aspidoscelis talpoideum: WF46L
Microbiota samples were processed through the ZymoBIOMICS Targeted Sequencing Service for 16S rRNA gene analysis. DNA was extracted using the ZymoBIOMICS-96 MagBead DNA Kit on an automated platform. The V3V4 region of the 16S rRNA gene was amplified with the Quick-16STM Primer Set, and libraries were prepared using the Quick-16S NGS Library Prep Kit. PCR products were pooled equimolarly after quantification with qPCR fluorescence readings, cleaned using the Select-a-Size DNA Clean & Concentrator, and further quantified with TapeStation and Qubit. Additional details are available at https://www.zymoresearch.com/pages/16s-its-amplicon-sequencing.