Whole Genome Bisulfite Sequence of cotton: leaves
Twelve BS libraries were constructed from the genomic DNA extracted from samples using a modified CTAB DNA extraction protocol . The libraries were sequenced and analyzed on the Illumina HiSeq 2500 platform (90 cycles) by BioMarker (Beijing, China). Clean reads were obtained from the raw data by removing adapters and low-quality reads and then mapped to the cotton reference genome . The methylation level (ML) of each sample was calculated using the formula.