GSM9630327: Ngn3OE; Mus musculus; ATAC-seq
5x104 FACS sorted cells were used for each ATAC-seq library construction. Cells were lysed using Lysis buffer (10mM Tris-HCl, 10mM NaCl2, 3mM MgCl2, 0.1% Igepal CA-30, 0.1% Tween 20). After lysis, nuclei were collected by 500g centrifugation for 5 minutes at 4 ℃, and immediately resuspended in Tn5 fragmentation reaction mixture (Vazyme) at 37 ℃ for 30 min. Fragments were then purified using VAHTS DNA clean Beads (Vazyme), and proceeded for library construction by PCR using TruePrep Index Kit V2 for Illumina primers (Vazyme).