dko_3 (CRX1741313; CRA026145)
Total RNA was extracted from samples using VeZol Reagent (Vazyme Biotech Co., Ltd, R411) and first-strand cDNAs (20 microL) were synthesized from 1 microg starting total RNA using a reverse transcription kit (Vazyme Biotech, R223-01433-01) according to the manufacturer's instructions. For RT-qPCR, the reaction mixture was composed of the 0.7 microL cDNA template, 0.2 mM primers, and 5 microL SYBR Green Mix (Vazyme Biotech, Q121-02/03) in a final volume of 10 microL.