GSM8563966: LNCaP extracted RNA, DMSO; Homo sapiens; OTHER
RNA was extracted from LNCaP cells, cDNA was generated by MaP RT primed with a DRAIC RNA-specific primer Cells were lysed with proteinase K, and RNA was extracted with PCA and buffer exchanged into folding buffer over a column. cDNA was generated by MaP RT with Superscript II including a DRAIC-specific primer. Final sequencing libraries were generated by PCR using DRAIC-specific primers, with indexing barcodes added in a second round of PCR.