minoxidil treatment zebrafish HSPCs RNA-seq treated 3 (CRX1755817; CRA026464)
RNA was extracted immediately after flow cytometry using Triton-based lysis. cDNA libraries were constructed using the TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme, TD502), with an average fragment size of approximately 350 bp. The resulting libraries were subjected to mini-bulk RNA sequencing and sequenced on the DNBSEQ-T7 platform.