A082_21d_H3K18la
ChIP-seq analysis were conducted using a Hyperactive Universal CUT&Tag Assay Kit (#TD903, Vazyme Biotech) following the manufacturers protocols . Briefly, BMDMs were collected and bound to the ConA-coated beads. Cells were then resuspended in antibody buffer and sequentially incubated with primary antibodies against histone modifications, including H3K18la (PTM Bio, Cat#PTM-1406), H3K27ac (Abmart, Cat#T59439M), and H3K4me3 (Abmart, Cat#MB9009), followed by incubation with secondary antibody (Goat anti-Rabbit IgG H&L, #AB206-01-AA, Vazyme Biotech). Subsequently, samples were incubated with pA-Tn5 transposase (Hyperactive PG-TN5/PA-TN5 Transposon) to mediate transposon activation and tagmentation. Fragmented DNA was isolated, amplified by PCR, and purified using VAHTS DNA Clean Beads (N411, Vazyme Biotech). Libraries were constructed with the TruePrep Index Kit V2 for Illumina. Library quality control included assessment of fragment size distribution and effective concentration using the Quant-iTTM dsDNA HS Assay Kit (Invitrogen, MA, USA) and Agilent 2100 Bioanalyzer (Agilent Technologies). Library quantification was performed with the VAHTS Library Quantification Kit for Illumina before sequencing on an Illumina Novaseq platform with 150 bp paired-end reads.