GSM8987436: A2780 cells, Control1; Homo sapiens; RNA-Seq
Total RNA was isolated, and mRNA was enriched using oligo(dT) magnetic beads, as described in the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (#E7770L). The isolated mRNA was fragmented and primed at 94°C for 15 minutes and subsequently processed for first- and second-strand cDNA synthesis. Following end-repair and adapter ligation, adaptor-ligated DNA was amplified using NEBNext Ultra II Q5 Master Mix under optimized PCR conditions based on RNA input. Library purification was achieved using SPRIselect beads (Beckman Coulter) and assessed for quality on an Agilent Bioanalyzer DNA Chip. Sequencing was performed on an Illumina platform with 2x150bp paired-end reads