Human gut metagenome from MIAB sample 99, Zymo_Three_Peaks extraction, replicate 2, LoopSeq synthetic long reads
Human gut metagenomic DNA extracted using Three-Peaks method (magnetic bead and manual extraction), technical replicate 2. LoopSeq synthetic long-read (SLR) library prepared by Element Biosciences using Amplicon LoopSeq for AVITI protocol. Input DNA concentration 1 ng in 30 L, sheared using Covaris g-tube. LoopSeq assigns unique molecular identifiers (UMIs, 18-bp barcodes) to DNA fragments, enabling reconstruction of contiguous reads from multiple short reads sharing a common barcode. Each sample assigned dedicated lane on Element AVITI sequencer. Supported SLR length range: 0.5-5 kbp.