GSM8564892: Control_2; Homo sapiens; RNA-Seq
Total RNA was extracted from the tissue samples, and the concentration and purity of the extracted RNA were detected by Nanodrop2000, RNA integrity was detected by agarose gel electrophoresis, and RQN values were determined by Agilent5300. The requirements for a single library were 1 μg of total RNA, concentration ≥ 30 ng/μL, RQN > 6.5 and OD260/280 between 1.8 and 2.2. ① Extraction of total RNA ② Oligo dT enrichment of mRNA ③ Fragmentation of mRNA ④ Reverse synthesis of cDNA ⑤ Ligation of adapter ⑥ Fragmentation screening and library enrichment ⑦ Sequencing on the NovaSeqX plus platform