GSM9556584: RNA-seq from 029 Pre Treatment Rep 2; Homo sapiens; RNA-Seq
RNA was purified using mechanical disruption and column purification. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.