GSM8502491: UT_1; Mus musculus; RNA-Seq
FLAMs with and without TGFβ were plated in 6-well plates at 1 x 106 cells/well and treated with Pam3  for 18 hours. We used the Direct-zol RNA Extraction Kit (Zymo Research, Cat no. R2072) to extract RNA according to the manufacturer's protocol. Quality was assessed by the MSU Genomics Core using an Agilent 4200 TapeStation System. The Illumina Stranded mRNA Library Prep kit (Illumina, Cat no. 20040534) with IDT for Illumina RNA Unique Dual Index adapters was used for library preparation following the manufacturer's recommendations but using half-volume reactions. Qubit™ dsDNA HS (ThermoFischer Scientific, Cat no. Q32851) and Agilent 4200 TapeStation HS DNA1000 assays (Agilent, Cat no. 5067-5584) were used to measure quality and quantity of the generated libraries. The libraries were pooled in equimolar amounts, and the Invitrogen Collibri Quantification qPCR kit (Invitrogen, Cat no. A38524100) was used to quantify the pooled library.