GSM9595256: sEVs, HFD-fed Sirt3 AKI replicate 3; Mus musculus; miRNA-Seq
The serum from mice were incubated with 0.5 volumes of Total Exosome Isolation Reagent (Invitrogen) overnight at 4 °C. After centrifugation at 10,000 g at 4 °C for 1 h, the sEVs-containing pellet was collected and resuspended in PBS for sequencing RNA libraries were prepared for sequencing using standard Illumina protocols