GSM9553465: MEG-01 control (pLKO.1) rep3; Homo sapiens; RNA-Seq
Total RNA was extracted from MEG-01 cells for each biological replicate using TRIzol reagent according to the manufacturer's instructions. Residual genomic DNA was removed with DNase I treatment. RNA quantity and purity were assessed by NanoDrop, and RNA integrity was assessed by Bioanalyzer. High-quality RNA was used for library preparation. RNA-seq libraries were prepared from [~1 µg] total RNA per sample using an Illumina-compatible library preparation workflow. Briefly, RNA was enriched poly(A) selection fragmented, and reverse-transcribed to cDNA, followed by end repair, adapter ligation with unique dual indexes, and PCR amplification. Library size distribution and concentration were evaluated by Bioanalyzer, pooled, and sequenced in paired-end mode on Illumina instrument model.