GSM8851507: TMJ condyles of 1-month-old C57BL6/J mice; Mus musculus; RNA-Seq
scRNA sequencing was performed on condyle of 1month wild-type C57BL/6J mice (n=20). The condylar bone and cartilage was cut into 1mm3 under a microscope. They were then digested in DPBS solution with 0.25% trypsin (Hyclone, cat. No. SH30042.01), 10 ug/mL recombinant DNAse I (RNase-free) (TaKaRa, cat. No. 2270B), and 5% fetal bovine serum (Thermo Fisher Scientific, cat. No. SV30087.02) for 30 minutes at 37°C. The cell suspension was filtered through a 40-μm strainer and erythrocytes were removed using a lysis reagent (Thermo Fisher Scientific, cat. No. 00-4333-57). Disscociated single cells were stained for viability assessment with AO/PI using a Countstar Fluorescence Cell Analyzer. Cells were loaded into Chromium Single Cell 3' V3.1 Kit (10X Genomics, Pleasanton, CA) and remaining steps followed the manufacturer's instructions.