GSM9336712: ΔmmpE BCG infected THP-1 macrophages, 24h, rep2; Homo sapiens; RNA-Seq
Total RNA was extracted using Trizol reagent (Invitrogen) according to the manufacturer's instructions. RNA integrity was assessed using Agilent 2100 Bioanalyzer. mRNA was enriched using Oligo(dT) magnetic beads, fragmented, and converted to cDNA using the NEBNext Ultra II Directional RNA Library Prep Kit. Second-strand cDNA was synthesized incorporating dUTP. After end repair, A-tailing, and adapter ligation, libraries were treated with USER enzyme and PCR-amplified. Library quality was assessed by Qubit and Agilent 2100 Bioanalyzer. Sequencing was performed on an Illumina NovaSeq 6000 platform with paired-end 150 bp reads (PE150).