Metagenomic sequencing of fecal samples from diarrheic cats
Total RNA was extracted from the samples. The extracted RNA was reverse-transcribed into complementary DNA (cDNA). Double-stranded cDNA was then synthesized through second-strand synthesis. The resulting double-stranded cDNA was amplified by random PCR. Sequencing libraries were prepared using the Nextera XT Library Prep Kit (Illumina, USA) according to the manufacturers protocol.