GSM8447250: BMDM WT LPS treated sample 2; Mus musculus; RNA-Seq
Cells were preserved in RLT buffer with β-mercaptoethanol and total RNA was later isolated with the Maxwell RSC48 instrument and miRNA Tissue Kit (Promega, Fitchburg, USA). 200 ng of total RNA were captured on oligo-dT beads and reverse transcribed directly on the beads, followed by the synthesis of the second cDNA strand after adapter ligation. The adapted cDNA was eluted and then amplified with barcoded primer. The finalized, barcoded libraries were pooled and sequenced on an Illumina NextSeq 2000 (Illumina, San Diego, CA, USA) with a read length of 60 nucleotides in paired-end mode