GSM8502809: CT1-2_rep3; Homo sapiens; RNA-Seq
Total RNA isolation used manufacturing protocol indicated by Sigma-Aldrich RNAzolRT R4533. Qubit RNA BR Assay kit (Q10211) was used to accurately measure RNA concentration. For RNA library synthesis, in brief, purified mRNA was cleaned using the NEBNext Poly(A) mRNA Magnetic Isolation Module kit (NEB E7490) and final library generation was created with the NEBNextÒ Ultra RNA Library Prep Kit for IlluminaÒ (NEB E7530) following manufacturing instructions. Barcoding came from TruSeq Unique Dual Indexes (Illumina, San Diego, CA) QubitÔ 1x dsDNA HS Assay kit (Q33231) was used to measure synthesis of the library.