GSM9046191: TIL391; Homo sapiens; OTHER
RNA was harvested using Rneasy mini kit (Quiagen). 10ng of input RNA was used for the construction of  TCR libraries. NGS libraries were prepared using AmpliSeq for Illumina TCR beta-SR Panel. The quality of TCR libraries was assessed using Bioanalyzer2100-High Sensitivity DNA kit (Agilent) and Qubit Fluorometric – Qubit dsDNA HS Assay kit (ThermoFisher Scientific).