GSM9630836: small RNA-seq mouse embryonic stem cell, 12 h time point, biological replicate 4; Mus musculus; miRNA-Seq
Cells were dissociated with Accutase, pelleted, and flash-frozen in liquid nitrogen. Pellets were stored at −80°C. Total RNA including small RNAs was extracted from frozen pellets using the miRNeasy mini kit (Qiagen) according to the manufacturer's instructions. Paired-end small RNA-seq libraries were prepared using the NEBNext Small RNA Library Prep Set for Illumina (New England Biolabs) according to the manufacturer's protocol. Libraries were sequenced on an Illumina NovaSeq 600 with 150 bp paired-end reads and a read depth of 4–15 million reads per sample.