GSM8564173: Anti-MBD1; Homo sapiens; OTHER
CUT&Tag assay was carried out using the NovoNGS CUT&Tag 3.0 High Sensitivity Kit (Novoprotein, N259-YH01) according to manufacturer's instructions. Briefly, 100000 lung cancer cells were incubated with ConA magnetic beads for 10min at room temperature. To amplify libraries, 35μL DNA was mixed with 2.5μL of a universal i5 and auniquely barcoded i7 primer. A volume of 10 μL 5× AmpliMix was added and mixed. The sample was placed in a Thermocycler with a heated lid using the following cycling conditions: 72 °C for 3 min (gap filling); 98 °C for 30 s; 18 cycles of 98 °C for 15 s, 60 °C for 20 s and 72°C for 10 s; final extension at 72 °C for 10 s and hold at 10 °C. libraried clean-up was performed NovoNGS DNA Clean beads.