Monokaryon M127
Genomic DNA was extracted using the Wizard Genomic DNA Purification kit and purified using the CHROMA SPIN columns of the Clontech kit. DNA was quantified by spectrophotometry and the quality was assessed on agarose gel. A minimum of 0.5 g of DNA was used for library preparation using the Illumina TruSeq Nano Kit. Paired-end sequencing was performed on an Illumina HiSeq 2500 sequencer using the HiSeq TruSeq SBS sequencing kit v.4.