16S analysis of nettle fertilizer; replicate S6
To sequence the V3-V4 region of the bacterial 16S rRNA gene, two-step, Nextera barcoded PCR libraries were generated using 341F (5- CCT ACG GGN GGC WGC AG -3) and 805R (5- GAC TAC HVG GGT ATC TAA TCC -3) with 20 PCR cycles for the first step and 10 PCR cycles for the second step.Subsequently the amplicon libraries were sequenced on an Illumina MiSeq using a v2 500 cycles kit.