TSWV isolate P342 on N. benthamiana
Libraries were constructed following the protocol of the NEBNext UltraII RNA Library Prep Kit for Illumina (New England Biolabs) or the Illumina DNA Prep Kit (Illumina), combined with the Maxima H Minus Reverse Transcriptase (ThermoScientific) and the NEBNext Ultra II Non-Directional RNA Second Strand Module (New England Biolabs) for double-strand cDNA synthesis. Both preparations included a ribosomal-RNA depletion treatment according to the QIAseq FastSelect -rRNA Plant Kit (Qiagen)