RNA-Seq of Cacao; DR1
Total RNA was isolated using the methods combined with RNeasy Mini kit Qiagen). Approximately 5 g total RNA from each cacao sample was used for RNA-seq library construction after checking its quality and quantity using Nanodrop ND-1000 (Thermo scientific, Wilmington, USA). RNA-seq libraries with an insert size of 300 bp were prepared independently for four cacao samples using an Illumina TruSeq RNA Sample Preparation Kit according to the manufacturers instructions. Libraries from the first replicate were sequenced using the Illumina NextSeq 500 platform with a PE read length of 150 bp at Phyzen (Seoul, Korea).