M. rosenbergii hepatopancreas 24h infected
Total RNA was extracted from hepatopancreas of Aeromonas caviae-infected M. rosenbergii at 24 hpi using TRIzol, with DNase I treatment. RNA quality was verified (Agilent 2100 Bioanalyzer). Libraries were prepared from 1 g RNA using the Illumina TruSeq RNA Sample Prep Kit with polyA selection, size-selected (200-300 bp), and sequenced on Illumina NovaSeq 6000 (150 bp PE). Raw reads were quality-trimmed with Trimmomatic and aligned to the M. rosenbergii genome (GCF_040412425.1) using HISAT2.