Metagenome of alfalfa silage
The extracted DNA was eluted in 60 L of elution buffer and the quality and concentration of that were determined through 1.0% agarose gel electrophoresis and NanoDrop 2000 spectrophotometer , respectively. The DNA was randomly fragmented by sonication for paired-end library construction. The qualified metagenome libraries were then subjected to high-throughput sequencing using Illumina NovaSeq6000 (Illumina Inc, San Diego, CA, USA) PE150 platform.