GSM9491404: E17_H3K4me3_HxD3; Mus musculus; OTHER
Extracted nuclei (nuclei extraction buffer: 20 mM HEPES-KOH pH 7.9, 10mM KCl, 0.5 mM fresh spermidine, 0.1% Triton X-100, 20% glycerol plus protease inhibitors) were bound to concanavalin A-coated magenetic beads and wash and permeabilize using C&R Wash Buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM spermidine, with 1x Roche complete protease inhibitor EDTA Free) with 0.05% Digitonin and 2 mM EDTA CUT&RUN was done using CUTANA ChIC/CUT&RUN kit from EpiCypher. CUT&RUN libraries were prepared NEBNext Ultra II DNA Library Prep Kit for Illuminal. All of DNA that was recovered following Phenol:Chloroform extraction was used as input.