GSM9368995: PFC, sleep deprived male mice 1; Mus musculus; RNA-Seq
Hippocampus or Prefrontal cortex were extracted immediately after the 5 hours of SD and flash-frozen in liquid nitrogen. Nuclei were extracted using 10x Genomics Chromium Nuclei Isolation with RNase Inhibitor Kit.  Extracted nuclei were further isolated by FANS. The libraries were constructed by using Chromium GEM-X Single Cell 3' Library Preparation Kit V3.1.