GSM8447014: 293T cells, control rep1; Homo sapiens; RNA-Seq
Total RNA was extracted from 293T cells using the TRIzol reagent following the manufacturer's instructions. The quality and integrity of RNA were assessed using an Agilent Bioanalyzer 2100 system. Poly(A) RNA was isolated from total RNA using oligo(dT) beads. RNA-seq libraries were constructed using the Illumina TruSeq RNA Library Preparation Kit. Libraries were indexed, amplified, and purified for sequencing.