16S rRNA sequencing Ulva australis Day 84_Stage_III_4
DNA was extracted using the DNeasy PowerSoil Kit. Bacterial communities were characterized by sequencing of amplicons for the 16S rRNA gene. Fragments of approximately 450 base pairs (bp) were amplified by PCR using the primer pair NOCHLFw (S-D-Bact-0341-b-S-17)/799F_rcRv. PCR products were sequenced using Illumina MiSeq technology (2 x 150 bp) at the Ramaciotti Centre for Genomics (UNSW, Sydney, Australia).