Longread sequencing of Carcinus maenas: putative inversion targeting
HMW extraction with Circulomics Nanobind Tissue Big DNA kit; library prep with Oxford Nanopore Cas9 sequencing kit and second round of custom Cas9 probes targeting putative inversion regions; sequencing on single flowcell on MinION mk1c - additional run with the same crab as 2a (flowcell flushed and re-loaded with the same library, then run for another 24 hours).