NextSeq 500 sequencing
3 Âµg of total RNA was used as input for ribosomal RNA depletion employing the PAN-Archaea riboPOOL kit (siTOOLS). Sequencing libraries were constructed with the Illumina Stranded mRNA Prep Ligation Kit, using dual indices. Libraries were PCR-amplified for 6 cycles and purified using AMPure XP Beads (Beckman-Coulter).