Mytilus edulis Vibrio bacterial challenge following noise exposure
Mytilus edulis Vibrio bacterial challenge following noise exposure
GGill tissue was dissected from mussels following 4 hours, 24 hours and 7 days of noise exposure and again at 48 hours recovery after the noise was turned off. Mussels were also sampled at 24 hours post-1E7 cfu/ml Vibrio splendidus bath challenge.   RNA was extracted from gill tissue using the RNeasy Blood & Tissue Kit (Qiagen) according to manufacturerâ€™s instructions.   Illumina, 2x150bp, strand specific and Poly-A selection. Performed by Genewiz from Azenta Life Sciences following manufacturer's protocol for Illumina sequencing. 