GSM8986586: GM00731, Sendai OSKM, day 0; Homo sapiens; RNA-Seq
Cells were dissociated using TrypLE, filtered through 40-μm cell strainers, resuspended in 0.04% BSA in PBS, counted, and adjusted to 1200 cells/μL. Cells were loaded onto Chromium X on Chromium Next GEM Chip G (10x Genomics). Libraries were generated using the Chromium Next GEM Single Cell 3' v3.1 (Dual Index) protocol (10x Genomics) as per manufacturer's instructions.