GSM9518526: EryP_KO_Stag2_Rep_1; Mus musculus; OTHER
CUT&RUN assays were performed using the CUTANA ChIC/CUT&RUN Kit (EpiCypher) on 200,000 EryPs (Stag2 WT or KO) for Stag2, following the manufacturer's instructions. After cell permeabilization and anti-Stag2 antibody binding, micrococcal nuclease (MNase) was activated to cleave DNA near bound protein sites, and released fragments were collected for downstream library preparation. Approximately 5 ng of CUT&RUN DNA per sample was processed using the EpiCypher CUT&RUN Library Prep Kit, again following standard protocols. After end repair and adapter ligation, library quantity and fragment size distribution were checked using an Agilent 4150 TapeStation (D5000 ScreenTapes).