GSM8501111: WNTi+FGF8 (batch A); Homo sapiens; RNA-Seq
Organoids were freshly collected at day 69 and dissociated with Papain; See Materials and Merthods in original paper scRNA-seq libraries were prepared with the Chromium Single Cell 3′ Library & Gel Bead Kit v.2 (10x Genomics, PN-120237). Final cDNA libraries were checked for quality and quantified using 2100 Bioanalyzer (High Sensitivity DNA Assay; Agilent Technologies).