GSM9629597: WT_dDAVP_48h_rep3; Mus musculus; RNA-Seq
Total RNA was extracted using Direct-zol RNA Microprep kit (R2062, Zymo Research) following the manufacturer's instructions. First-strand and double-stranded cDNA was synthesized and amplified from 1 ng of total RNA using SMART-Seq® mRNA HT Kit (634796, TAKARA). Amplified cDNA were purified using AMPure XP beads (A63880, Beckman Coulter), and quantified using a Qubit dsDNA HS DNA assay kit (Q32851, Invitrogen). One nanogram of the synthesized cDNAs were fragmented and tagged with index primers (FC-131-1024, Nextera XT DNA library Preparation Kit, Illumina) for high-throughtput sequencing. Libraries were purified by AmPure XP beads, and quantified using a Qubit dsDNA HS DNA assay kit. Equal amounts of indexed libraries were pooled and sequenced on an Illumina platform.