GSM8849291: Mel3_dsx_neurons; Drosophila melanogaster; RNA-Seq
For each replicate, the central nervous system of approximately 100 doublesex-GAL4 x UAS-nls::tdTomato flies were dissected in ice cold Schneider's medium and digested in a solution containing Liberase DH for 45 mins. The tissue was washed with Schneider's medium and broken apart via pipetting before FACS sorting out the tdTomato+ cells. Library was constructed according to the manufacter's instructions (single cell 3' V2 protocol, 10X Genomics)