GSM8708544: Sp5C, CFA injection+Tributyrin via oral gavage,rep2, GEX; Mus musculus; RNA-Seq
Cell nuclei were isolated from Sp5C tissues using the 10X Genomics Chromium Nuclei Isolation Kit (manual CG000505 Rev A). Tissue homogenization was performed in lysis buffer, followed by debris removal and filtration (Flowmi 40-μm). Isolated nuclei were counted using a Countess 3 FL Automated Cell Counter. Isolated nuclei were processed using the 10X Genomics Chromium Next GEM Single Cell Multiome ATAC + Gene Expression workflow (manual CG000338 Rev F). Nuclei were subjected to tagmentation, barcoded in the Chromium X Controller, and prepared for library construction using 10X Genomics PN-1000283. Libraries were sequenced on an Illumina NovaSeq platform.