GSM7656704: AKSP multiome - s.c GEX; Mus musculus; RNA-Seq
Matrigel domes containing the indicated organoid lines were collected and washed with cold cell recovery solution, then incubated in TryPLE  for 10 mins at 37C. Next, they were dissociated to single cells with gentle tritruation. Cells were stained and counted with Trypan blue to estimate the live/dead ratio. Single Cell Multiome ATAC and Gene Expression libraries were prepared according to the 10X Chromium Next GEM Single Cell Multiome ATAC + Gene Expression user guide for standard Illumina paired-end sequencing