BR2
In this study, metagenomic DNA samples isolated from boron mine soils were utilized. Following DNA purification, the library was prepared using the Illumina Nextera XT DNA Library Preparation Kit (Illumina, USA). The library preparation protocol encompassed the sequential steps of tagmentation, dual-indexed adaptor ligation, and subsequent PCR amplification.Post-PCR, the libraries underwent purification using AMPure XP magnetic beads to remove residual reagents and small fragments.The quality and fragment size distribution of the final libraries were assessed using an Agilent TapeStation 4200 instrument (High Sensitivity D5000 ScreenTape), while the molar concentration was determined using the Qubit 4.0 Fluorometer (Invitrogen). The prepared libraries were then pooled and sequenced on the Illumina MiSeq platform utilizing the MiSeq Reagent Kit v3 (600 cycles).