U3035-8Gy-220kV-3
Total RNA was extracted using the RNeasy Mini Kit (Qiagen, Santa Clarita, CA). RNA quality was confirmed via Qubit fluorometry and Agilent 2100 Bioanalyzer; only samples with RIN >7.0 were used. Libraries were prepared using the KAPA mRNA HyperPrep Kit and sequenced on the Illumina NextSeq 2000 platform (PE50, paired-end).