The Barrett's precursor state to oesophageal adenocarcinoma shares features with transient cell populations in the developing human oesophagus - snRNA-seq
The Barrett's precursor state to oesophageal adenocarcinoma shares features with transient cell populations in the developing human oesophagus - snRNA-seq
Human embryonic material was collected under ethical approval from the North West Research Ethics Committee (18/NW/0096), informed consent from all participants and according to the Codes of Practice of the Human Tissue Authority15. Tissue collection took place on our co-located clinical academic campus overseen by our research team ensuring immediate transfer to the laboratory. Embryonic material was staged by the Carnegie classification. Fetal material (after 56 dpc) was staged by foot length and ultrasound assessment. Individual tissues and organs were immediately dissected (Supplementary Data). In brief, describe the dissection. All visible adherent mesenchyme was removed under a dissecting microscope.  Used 10X sample collection protocol, detailed in CG000053_CellPrepGuide_RevC.pdfc Used 10X sample collection protocol, detailed in CG000053_CellPrepGuide_RevC.pdf