GSM9491433: Week 9 male rat venticle heart muscle, Wildtype control 1; Rattus norvegicus; RNA-Seq
Total RNA was extracted from frozen ventricular tissue using TRIzol reagent (Invitrogen) according to the manufacturer's protocol. The concentration and purity of the RNA samples were checked by Qubit fluorometer. The RNA integrity of the RNA samples was checked by Bioanalyzer. Then, we sent our qualified RNA sample to Sequencing Core Facility for library preparation and sequencing. The sequencing library was prepared using NEBNext Ultra II Directional RNA library Prep Kit and sequenced using Illumina Nextseq 500 platform to generate paired-end reads.