Ribo-Seq_ IFN_8h_sample_2
The lysate solution was subjected to nucleic acid digestion to separate ribosome-bound RNA from free mRNA, followed by enrichment of ribosome-mRNA complexes to obtain ribosome-protected fragments (RPFs). RNA fragments of approximately 35 nt in length were isolated and purified via PAGE gel electrophoresis. The recovered RNA underwent end repair, adaptor ligation at both ends and rRNA depletion, and was subsequently reverse-transcribed and amplified by PCR. The target library was then obtained through another round of PAGE gel purification and size selection. After passing quality control, the library was subjected to high-throughput sequencing.