GSM8987115: GR3-1; Homo sapiens; RNA-Seq
Total RNA was extracted from cells with a miRNeasy Mini kit (Qiagen) following the manufacturer's instruction. Ribosomal RNA was depleted using Ribo-Zero Plus rRNA Depletion Kit (Illumina). The linear RNA parts were digested with RNaseR. According to the manufacturer's instructions, library preparation was performed using a TruSeq stranded mRNA sample prep kit (Illumina, San Diego, CA) without the poly-A selection step. Libraries were converted to libraries for DNBSEQ using MGIEasy Universal Library Conversion Kit (App-A).