GSM9015937: scRNA-seq, SW620, 5x Abs, GEX; Homo sapiens; RNA-Seq
Media was removed from cells, which were washed with 10 mL PBS. Cells were then incubated in 5 mL Gibco enzyme-free dissociation buffer for ~15 minutes, then resuspended through gentle pipetting with an additional 5 mL of RPMI-1640 with supplelements. Libraries were formed according to the manufacturer's instructions for the Chromium Next GEM Single Cell 5' Reagent Kit v2 (10x Genomics). Cells were resuspended in master mix and loaded with partitioning oil and gel beads to generate the gel beads-in-emulsion (GEMs). The poly-A RNA in each GEM was converted to cDNA with the Illumina and 10x recognition sequences.  After being cleaned up with DynaBeads, the cDNA was amplified, selected for appropriately sized sequence fragments, and selected using SPRIselect reagent for library construction. The anti-glycan antibodies' library was was formed according to the manufacturer's instructions for the Chromium 5' Feature Barcode Kit (10x Genomics).