GSM8563695: brain, snRNA-seq multiple sclerosis, 9; Homo sapiens; RNA-Seq
For nuclei isolation, 20-30 mg of muscle tissue was chopped and homogenized using 2 ml lysis buffer (0.32 M sucrose, 5 mM CaCl2, 3 mM Mg(Ac)2, 0.1 mM EDTA, 10 mM Tris-HCl, 1 mM DTT, 0.5% Triton X100 in DEPC-treated water) and a glass Dounce homogenizer with a tight pestle on ice. Libraries were generated according to the provided protocols according to the 10X Next GEM 3' Kit v3.1 protocol. After recommended quality control, all libraries were pooled and sequenced on an Illumina NovaSeq 6000 system. Sequencing was performed at Institute of Clinical Biology (IKMB, Kiel).