GSM8383056: pancreatic islets, NCD_3; Mus musculus; miRNA-Seq
Total RNA from islets of NCD and HFD mice was isolated using the RNeasy mini kit (Qiagen), following the protocol for total RNA isolation. RNA integrity and concentration were assessed using an RNA Nano 6000 Assay Kit for the Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA), according to the manufacturer's instructions. Following the guidelines stipulated by the manufacturer, sequenced libraries were constructed with the NEBNext Ultra small RNA Sample Library Prep Kit for Illumina (NEB, USA). Index codes were added to attribute sequences to each sample. The index-coded samples were clustered with the assistance of a cBot Cluster Generation System equipped with a TruSeq PE Cluster Kitv3-cBot-HS (Illumia) following the directions stipulated by the manufacturer.