GSM8534719: ATAC-seq-H9_D16; Homo sapiens; ATAC-seq
ATAC-seq was performed using TruePrepTM DNA Library Prep Kit V2 for Illumina® (Vazyme), a total of 50,000-80,000 cell pellets were resuspended in 50 μL lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% (v/v) IGEPAL CA-630). The suspension was subjected to a transposition reaction and purification with a MinElute Kit (QIAGEN). After PCR amplification of transposed DNA fragments, the amplified libraries were purified using a QIAquick PCR (QIAGEN) column. Then, purified libraries were assessed by gel electrophoresis and Qsep100 (Bioptic)