GSM9631008: mRNA-seq mouse embryonic stem cell, 36 h time point, biological replicate 3; Mus musculus; RNA-Seq
Cells were dissociated with Accutase and pelleted. Total RNA was immediately extracted using the RNeasy kit (Qiagen) according to the manufacturer's instructions and stored at −80°C until sequencing. Paired-end strand-specific poly(A)-selected mRNA-seq libraries were prepared using Illumina TruSeq Stranded mRNA Sample Preparation Kit. Libraries were sequenced on an Illumina HiSeq 4000 instrument with 40 bp single-end reads (stranded) and an average read depth of approximately 40 million reads per sample.