GXG3207FB (CRX1751740; CRA026392)
Total community genomic DNA extraction was performed. And the V4-V5 hypervariable regions of the 16S rRNA gene was amplified. Subsequently, 22S gene library was constructed, and the samples were quantified and sequenced. By comparing with the RDP database, the OTUs representative sequences of bacteria in the samples were used for taxonomic identification. Finally, the microbial richness and diversity in the samples were compared, and their functions were predicted by comparing with the microbial genome database of known metabolic functions.