RNA-Seq of Gasterosteus aculeatus: adult
cDNA libraries were generated from 100 ng of RNA. Library preparation was carried out by purification of poly-A containing mRNA, mRNA fragmentation, random primed cDNA synthesis, adapter ligation, and adapter specific PCR amplification by INVIEW Transcriptome Discover. Illumina paired end read sequencing was carried out to generate 2x150 bp reads.