Whole genome sequencing of rockfish
Genomic DNA was extracted from preserved tissue. WGS libraries were created using a modified version of the Nextera library prep, with approximately 1/20 of reagents used. DNA fragmentation was completed usign tagmentation. Libraries were dual-indexed and included a total of 12 PCR cycles. Individual libraries were normalized using a SequelPrep Normalization plate. Library size was selected using 0.6X ampure beads.