GSM8783402: np_control4, pancreatic isltes, non-pregnant, vehicle treatment; Mus musculus; RNA-Seq
Mouse pancreatic islets were isolated after collagenase P (Sigma-Aldrich 11213865001) injection through the pancreatic duct, followed by digestion and separation by density gradient using Histopaque-1077 (Sigma-Aldrich 10771). Total RNA was extracted from pancreatic islets by using the RNeasy Micro Kit (QIAGEN, Germantown, MD, USA) RNA was quantified by Qubit 2.0 Fluorometer (ThermoFisher Scientific, Waltham, MA, USA) and integrity checked with TapeStation 4200 (Agilent Technologies, Palo Alto, CA, USA). rRNA depletion libraries were prepared using QIAGEN FastSelect rRNA HMR Kit (Qiagen, Hilden, Germany) and NEBNext Ultra II RNA Library Preparation Kit for Illumina HiSeq (NEB, Ipswich, MA, USA). Libraries were validated using the Agilent TapeStation 4200 and quantified by Qubit 2.0 Fluorometer and quantitative PCR (KAPA Biosystems, Wilmington, MA, USA). Samples were sequenced with a 2x150bp paired-end configuration