Single-nucleus sequencing of rice at the flowering stage of the node
SnRNA-seq libraries were prepared using the Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (10x Genomics) on a Chromium Controller. Nuclei were isolated from node I and resuspended in PBS containing 0.04% BSA. Nuclei from each developmental stage were separately loaded onto the Chromium Next GEM Chip G to generate single-cell gel beads in emulsion (GEMs), according to the manufacturer's instructions. The captured nuclei were subsequently lysed, and the released RNA underwent reverse transcription to produce barcoded, full-length cDNA. Libraries were constructed following the protocol provided by the reagent kit. The quality of the libraries was assessed using the Qubit 4.0 Fluorometer and the Agilent 2100 Bioanalyzer. Sequencing was performed on the Illumina NovaSeq 6000 platform, achieving a sequencing depth of at least 50,000 reads per nucleus, with paired-end 150 bp (PE150) reads.