GSM9518079: AML JH4198, WGBS; Homo sapiens; Bisulfite-Seq
Primary AML samples were collected from peripheral blood or bone marrow. WGBS libraries for CMP and MEP normal controls were generated using the IDT Methyl-Seq Kit. For primary AML samples, and CD34+ and GMP normal controls, WGBS libraries were generated using NEBNext Ultra DNA library Prep kit for Illumina according to the manufacturer's instructions with modifications. Input gDNA was spiked with 1% unmethylated Lambda DNA (Promega) to monitor bisulfite conversion efficiency. Size selection was performed using SPRIselect beads and insert sizes of 300-400bp were isolated. Samples were bisulfite converted after size selection using EZ DNA Methylation-Lightning Kit (Zymo). Libraries were sequenced on an Illumina HiSeq4000 or NovaSeq6000 instruments.