GSM8502517: small_RNA_Saline_M2MC_Haplosufficient_4; Mus musculus; ncRNA-Seq
Cells were sorted from Miwi2+/tom and Miwi2tom/tom mice into Qiagen buffer RLT supplemented with 1% beta-mercaptoethanol. RNA  was isolated on the Qiagen QIASymphony SP/AS using the QIAshymphony RNA kit and the miRNA CT 400 protoocol. The RNA-Seq libraries were prepared using Illumina Stranded Total RNA with RiboZero Plus kit per the manufacturer instruction.  The small RNA-Seq libraries were prepared using Qiagen QIASeq small RNA kit per the manufacturer instruction.