Mytilus edulis Vibrio bacterial challenge following noise exposure
Mytilus edulis Vibrio bacterial challenge following noise exposure
Gill tissue was dissected from mussels and immediately placed on dry ice following either control conditions or 7 days of noise exposure with 48 hours recovery after the noise was turned off. Mussels within each of these groups were also subjected to either control conditions (uninfected) or subjected to 24 hours 1E7 cfu/ml Vibrio splendidus bath challenge for 24 hours.  RNA was extracted from gill tissue using the RNeasy Blood & Tissue Kit (Qiagen) according to manufacturerâ€™s instructions.   Illumina, 2x150bp, strand specific and Poly-A selection. Performed by Genewiz from Azenta Life Sciences following manufacturer's protocol for Illumina sequencing. 