RNA sequencing of DU145 (a human prostate cancer cell line) NEK9 genetically Knocked out cell line compared to its wildtype parental counterpart
RNA sequencing of DU145 (a human prostate cancer cell line) NEK9 genetically Knocked out cell line compared to its wildtype parental counterpart
DU145 NEK9 WT and KO cells were cultured in cell culture conditions in DMEM (10%FBS) . Cells were trypsinized and subjected to RNA isolation. Total RNA was isolated using Direct-Zol RNA miniprep (R2052) RNA isolation kit. To prepare an RNA-Sequencing library, 500ng of total RNA was used to isolate mRNA using magnetic beads (PolyA mRNA Isolation Module, NEB Cat No# E7490L). The RNA-Seq library was then prepared using an mRNA library preparation kit (NEB Cat No# E7770L) strictly following the vendorâ€™s recommended protocol. Following library preparation, the library concentration was measured using Qubit 2.0 (Invitrogen), and the appropriate fragmentation sizes were confirmed using Bio-analyzer (Agilent Cat No# 5067-5582).