GSM9015207: HaCaT cells DNA, PGK AAV candidate enhancer library, biol rep 3; Homo sapiens; OTHER
DNA and RNA were extracted using the ALLprep 96 DNA/RNA kit (QIAGEN, Hilden, Germany). 2 µg of total RNA were used for mRNA enrichment utilizing the NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Ipswich, USA) followed by cDNA synthesis and amplicon PCR using enhancer cassette-specific primers. 20 ng of PCR product as well as 20 ng of DNA were used for index PCR with UDI primers (New England Biolabs, Ipswich, USA), which bind inside the enhancer cassette. Additionally, 5 ng and 10 ng of plasmid and viral input DNA were used for index PCR, respectively.