HIF2a C0 KO rep1
Libraries were performed with NovoNGS CUT&Tag kit (Novoprotein) following the manufacturers instructions. Stem-like Th2 or pathogenic Th2 cells were incubated with IgG isotypes or antibodies against HIF2 (Novus Biologicals) or GATA3 (BD Bioscience), to minimize non-sepcific binding, HIF2 or GATA3-deficient cells were included. Adapter ligated DNA fragments were amplified by 25 cycles of PCR.