GSM8502677: EVT-rep3; Homo sapiens; ATAC-seq
ATAC-seq library preparation was performed on 1 × 106freshly cultured cells using a commercially available ATAC-Seq kit from Active Motif (#53150, Carlsbad, CA). We followed manufacturers protocol with some minor modification. Each sample was lysed in the ATAC-seq lysis buffer. Next, the samples were processed for the transposase reaction. After cleanup of the transposed DNA, samples were stored at −20 °C until library amplification. Samples were subsequently thawed at room temperature and library construction completed according to manufacturer protocol. Libraries were quantified using a Qubit dsDNA BR Assay Kit (Q32853, Thermo-Fisher) and the size was determined with a High Sensitivity DNA Bioanalyzer Kit (5067-4626, Agilent, Santa Clara, CA) and sequenced on a NovaSeq 6000 (Illumina, San Diego, CA) using Nextera Sequencing primers.