SAM24431193
Library was performed according to the manufacters instructions (single cell v2 3.1 chemistry protocol, 10x Genomics). Briefly, cell suspensions were resuspended in the master mix and loaded together into wells of the 10X Chip with partitioning oil and gel beads. Gel bead-in-emulsion (GEM) containing poly-A RNA were reverse transcribed into cDNA containing the Illumina primer sequences, cell barcodes and Unique Molecular Identifiers (UMIs). Pooled cDNA were then cleaned up using Dynabeads and amplified by PCR. SPRIselect reagent was used to select appropriate size fragments for gene-expression and hashtag antibody reads for libraryconstruction. Libraries were made according to the 10X protocol with the R2 primer sequence, paired-end constructs with P5 and P7 sequences, and a sample index for library.