Mimics21-2
The extracted mRNA was enriched using mRNA Capture Beads and fragmented at a high temperature. Using the fragmented mRNA as a template, first-strand cDNA was synthesized in a reverse transcriptase mixture, followed by simultaneous second-strand cDNA synthesis, end repair, and dA-tailing. Next, adapters were ligated, and target fragments were purified using Hieff NGS DNA Selection Beads. Finally, the libraries were amplified by PCR and sequenced after passing quality control assessment.