GSM8988609: RNA-seq, KO, DP thymocytes, rep.2; Mus musculus; RNA-Seq
Total RNA was extracted from FACS-purified DP thymocytes using TRIzol reagent (Thermo Fisher Scientific). RNA integrity number was evaluated using an Agilent 2100 Bioanalyzer with the RNA 6000 Pico Kit (Agiletn), and samples with RNA integrity number > 8 were used for library preparation. RNA-seq libraries were constructed from 10 ng of total RNA using the SMART-seq Standard Kit (Takara).