MYB92_1-MYB92_1_1 (CRX1707783; CRA025479)
Genomic DNA was sheared into fragments ranging from 100 to 400 bp using a Covaris M220. These fragments were then used to construct libraries with the NEXTFLEX Rapid DNA Seq Kit. The coding sequence of MYB92 was cloned into the pFN19K HaloTag T7 SP6 flexi expression vector, and the resulting halo-tagged fusion protein was induced and subsequently purified. This fusion protein was then co-incubated with the genomic DNA, followed by the isolation of MdSPL6-bound DNA sequences. The assay included two replicates (MYB92-1 iand MYB92-2) and one negative control(MYB92-input).