Illumina WGS of Podocoryna genome strain father: adult male non-reproductive polyps
200 non-reproductive polyps from PCLH003 were incubated in penicillin-streptomycin, starved for four days, and dissected for high molecular weight DNA extraction. After washing in ASW and removing excess seawater, tissues were ground in liquid nitrogen with UEB buffer and phenol:chloroform:isoamyl alcohol. DNA was precipitated with isopropanol, washed with ethanol, and resuspended in TE buffer. Quality was assessed using Agilent Tapestation, and a standard Illumina WGS library was generated and subsequently sequenced on the Illumina HiSeq 2000.