GSM8816902: P2.5_49; Mus musculus; RNA-Seq
Each sample is lysed using a 2 µL lysis buffer with 0.2% TritonX-100 and 40 U/mL Recombinant RNase Inhibitor, which disrupts cell membranes to release RNA and other cellular contents while preventing RNA degradation. Transcriptome libraries were prepared following the Smart-seq2 protocol. Sequencing libraries were constructed by using KAPA HyperPlus Kit according to the manufacturer's instructions.  All libraries were sequenced on the Illumina NovaSeq 6,000 platform with 150-bp paired-end mode.