GSM9555676: ADAR1 #2; Homo sapiens; RNA-Seq
Total RNA was harvested in 200 μL 1X Buffer RLT (QIAGEN, 79216) and isolated using the QIAGEN RNeasy Mini Kit (74104), followed by 1 hr of DNase treatment using TURBO DNase (ThermoFisher, AM2238). DNase-digested RNA was then further purified with the Zymo RNA Clean and Concentrator-25 kit (R1017). RNA was hydrolyzed in bicarbonate buffer and the reaction was stopped by ethanol precipitation. The resulting fragments were then end-repaired and converted into an Illumina library using the NEB Next Small RNA Library Prepkit (Bioke, E7300S), according to the manufacturer's protocol.