Identification of DNA viruses from a fecal samples of a ferel pigeon (Columba livia domestica) in Tempe Arizona, USA
A pigeon fecal samples were collected from Kiwanas park (Tempe, Arizona). 5g of the sample was resuspended in 2 ml of SM buffer and 1 ml was filtered through a 0.22um syringe filter. 200 ul of the filtrate was then used to extract viral DNA using the High Pure Viral Nucleic Acid kit (Roche Diagnostics, USA). Circular molecules in the viral DNA extraction were enriched using rolling-circle amplification (RCA) with the TempliPhi kit (GE Healthcare, USA).