GSM9195832: cKO 2; Mus musculus; RNA-Seq
Total RNA from 10,000 sorted PCs was extracted using the EZ-press RNA Purification Kit PLUS (EZB, B0004-PLUS-S). Reverse transcription, PCR preamplification, and purification of PCR products were conducted using the Discover-sc WTA Kit V2 (Vazyme Biotech). The RNA-seq library was generated by TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme Biotech) using 10 ng amplified cDNAs as input following the manufacturer's protocols.