GSM8708252: S38; Homo sapiens; RNA-Seq
RNA was isolated from whole cell lysates using RNeasy Mini kit (Qiagen, 74106). Sequencing was conducted at the McMaster Genomics Facility, Farncombe Institute at McMaster University. RNA quality was first verified using a Bioanalyzer (Agilent), followed by poly(A) enrichment (NEBNext Poly(a) mRNA Magnetic Isolation Module; NEB). Library were prepared (NEBNext Ultra II Directional RNA Library Prep Kit; NEB) and library fragment size distribution was verified (Agilent TapeSection D1000; Agilent). Libraries were quantified by qPCR and pooled in equimolar amounts. Subsequently, qPCR and fragment size distribution were verified. Libraries were then sequenced on an Illumina HiSeq 1500 across 12 HiSeq Rapid v2 flow cells in 6 lanes (Illumina) using a paired end, 2x50 bp configuration, with onboard cluster generation averaging 80M clusters per biological replicate.