GSM9096908: OCI-AML-3 H1-3 KO clone 1 cells, H3K9me3 antibody (ChIP); Homo sapiens; ChIP-Seq
Cells were fix with 1% formaldehyde. Cell lysates were clarified from sonicated nuclei using a Diagenode Bioruptor, and histone-DNA complexes were isolated with antibody incubated over-night. Rabit IgG (Cell signaling; #2729S) was used as a control. Genomic DNA was purified using the i-Pure V2 kit (Diagenode) Libraries were generated using the MicroPlex Library Preparation Kit (Diagenode) following the manufacturer's instructions and analyzed on a 2100 Bioanalyzer system (Agilent) prior sequencing