GSM8536520: ChIP-WT-CTCF-rep1; Mus musculus; ChIP-Seq
ChIP assay was performed using Active Motif's ChIP assay kit (53035) according to manufacturer's instructions with slight modifications ChIP samples were prepared according to the Active Motif protocol. In brief, CD8+ T cells were activated with anti-CD3/CD28 for 48 h, after 24 h of infection with Irf8-HA, infected CD8+ T cells were purified and cultured in the presence of IL-2 (10U/mL) for another 48 h, then re-stimulated with PMA/Ionomycin for 2 h and fixed with 1% formaldehyde at a concentration of 1 million cells/mL. ChIP assay was performed using Active Motif's ChIP assay kit (53035) according to manufacturer's instructions with slight modifications. A total of 1 x 107 cells were lysed and digested with shearing enzyme followed by sonication. Antibodies against HA tag (3724, clone C29F4; Cell Signaling Technology) and anti-CTCF (CST, catalog 3418) was added to supernatant followed by Dynabeads Protein A (LifeTechnologies) pulling down target fragment.