GSM8708002: Dtx2+/+ MEF cells-Rep2; Mus musculus; RNA-Seq
Cells were subjected to total RNA extraction using TRIZOL reagent (Invitrogen) Library preparation and sequencing were finished by LC-Bio Technology CO., Ltd., Hangzhou, China. The protocol is as follows: 1) RNA fragment and reverse transcription; 2) End repair, add A and adaptor ligation; 3) PCR; 4)Single strand separation and cyclization; 5) DNA nanoball synthesis; 7) Sequencing.