Genome 14
Sample libraries were prepared using the PCR-free Oxford Nanopore Technologies (ONT) Ligation Sequencing Kit (SQK-NBD114.24) with the NEBNext Companion Module (E7180L) to manufacturers specifications. No additional DNA fragmentation or size selection was performed.Nanopore sequencing was performed on an ONT GridION or PromethION sequencer using R10.4.1 flow cells in one or more multiplexed shared-flow-cell runs. Run design utilized the 400bps sequencing mode with a minimum read length of 200bp. Adaptive sampling was not enabled.Guppy1 (v6.5.7) was used under the super-accurate (sup) and 5mC basecalling models (dna_r10.4.1_e8.2_400bps_modbases_5mc_cg_sup.cfg). Default trim options were not changed, resulting in the trimming of detected adapters, primers, and barcodes. Samtools fastq2 (v1.20) was used to generate the FASTQ file from the BAM file. Sequencing statistics are included in the DNA Sequencing Stats.xlsx file.