GSM8536746: vancomycin_end_1; Homo sapiens; RNA-Seq
Total RNA was extracted using standard trizol protocol, following RNA concentrations were measured with Qubit Flourometric Quantification (Theromo Fisher Scientific), RNA integrity number (RIN) was determined with Bioanalyzer on RNA 6000 Nano Kit (Agilent Technologies), all samples were high quality with RIN > 9. RNA-seq libraries were generated using NEBNext Poly(A) + ultra–Directional RNA library Prep Kit for Illumina (New England BioLabs) according to manufacturer's instructions.  For sequencing (150 bp paired-end) at Genewiz NJ, USA