GSM9517983: Embryonic day 16, ID F1; Mus musculus; RNA-Seq
Total RNA was extracted from the cerebral cortices of wild-type (WT) mice at embryonic day 16 (E16), postnatal day 5 (P5), and postnatal day 10 (P10) using the Qiagen RNeasy Mini Kit (50), following the manufacturer's instructions. Immediately after dissection, tissues were snap-frozen on dry ice and stored at −80 °C until RNA extraction. On-column DNase I digestion was performed to eliminate residual genomic DNA. RNA concentration and purity were measured using a NanoDrop spectrophotometer (Thermo Fisher Scientific), and RNA integrity was assessed using an Agilent 2100 Bioanalyzer. Only high-quality RNA with an RNA Integrity Number (RIN) of 9 or higher was used for sequencing. Total RNA was extracted from WT cortices at E16 (n=7), P5 (n=5) and P10 (n=8) as described above for a total of 20 samples. Only high-quality RNA with an RNA Integrity Number (RIN) of 9 or higher was used. Library preparation and sequencing have been performed with TruSeq RNA Library Preparation Kit v2 (Illumina) by Genewiz.