GSM8446840: Schmidtea mediterranea, whole-body, unperturbed conditions, scATAC-seq, batch 1; Schmidtea mediterranea; ATAC-seq
Animals were flash-frozen in liquid nitrogen and resuspended in cold lysis buffer. Animals were dissociated by smashing on a cell strainer with the aid of a syringe plunger. The resulting nuclei suspension was resuspended in 0.04% BSA, and later resuspended in tagmentation buffer and custom-loaded Tn5 transposase. Tagmented DNA was purified using the Monarch PCR DNA Cleanup kit following manufacturer's instructions, and amplified using PCR with Kapa HiFi HotStart ReadyMix (Roche). The library was sequenced in a NovaSeq X Plus PE150 Illumina sequencer.