GSM8535388: Mutant Dnmt3a/Npm1/Nras; Mus musculus; RNA-Seq
CD117+ hematopoietic cells were enriched from healthy or malignant mouse spleens using magnetic-activated cell sorting (MACS). In each experiment, cells were washed on Laminar Wash MINI or HT2000 System (Curiox Biosystems Inc) with the following settings: 25 cycles, flow rate of 10uL/s, initial volume of 55uL. Pooled cells were captured by 10X Chromium X 3' version 3.1 kit (1000268, 10X Genomics).