16S V4 of synthetitc metagenome
Fecal DNA was amplified targeting the V4 region of the 16S rRNA gene using primers 515F (5-GTGCCAGCMGCCGCGGTAA-3) and 806R (5-GGACTACHVGGGTWTCTAAT-3). Each sample was amplified in two independent PCR replicates and each replicate. Primers included frameshifting nucleotides was sequenced. Sequencing runs included positive controls (ZymoBIOMICS Microbial Community DNA Standard)