GSM8563761: WT Male Th2 2; Mus musculus; RNA-Seq
RNA was extracted from purified cell populations using QIAzol and miRNeasy RNA extraction kits (QIAGEN) according to manufacturer's instructions. RNA yield was quantified and checked for quality using a Nanodrop spectrophotometer before quality control and further quantification using an Agilent bioanalyser prior to RNAseq. Pooled cDNA libraries were sequenced on one lane of an Illumina Novaseq 6000 S4 flow-cell at 100PE