RNAseq of H.sapiens: TC71 Ewing cell line + 10uM ABC, 24h
RNA was isolated from Ewing sarcoma cell lines treated with 10 uM abacavir (ABC) for 24h, using the mirVana RNA kit (ThermoFisher, AM1560). RNAs were extensively treated with Turbo DNase (ThermoFisher, AM1907) to remove genomic DNA. RNAseq libraries were prepared with the SMARTerStranded Total RNA-Seq Kit v3 - Pico Input Mammalian formulation (634356) and the Takara SMARTer RNA unique Dual index kit  96U (634457) and paired-end sequenced on the Illumina NovaSeq X Plus sequencer.