GSM8708345: C18_iHO_P0; Homo sapiens; RNA-Seq
iPSC-derived organoids (under iHO and FOcondition) embedded in Matrigel were washed by ice-cold PBS and dissociated into single cells by using trypsin/EDTA(0.05%) for 10-20 minutes. Cell viability was confirmed to be over 80 % by staining trypan blue. Viable cells (over 5000) underwent scRNA-seq library preparation and were sequenced using the 10x Genomics Chromium platform, according to the manufacturer's protocols. Libraries were prepared using Single Cell 3' v2 Reagent Kit(10X Genomics)