GSM9518138: CD34-1, scATAC-seq; Homo sapiens; ATAC-seq
Cryopreserved samples were used to generate a single cell suspension (for scATAC-seq, according to protocol Nuclei Isolation for Single Cell ATAC Sequencing [10x Genomics]) or a single nuclei suspension (for single-cell Multiome, according to protocol Nuclei Isolation for Single Cell Multiome ATAC + Gene Expression Sequencing [10x Genomics]). Single cells (for scATAC-seq) or nuclei (for scMultiome ATAC+Gene Expression) were captured on a 10x Chromium Controller instrument according to manufacturer instructions. Single-cell ATAC libraries were constructed with Chromium Next GEM Single Cell ATAC reagents. Single-cell multiome ATAC libraries were generated using the Chromium Next GEM Single-cell Multiome ATAC + Gene Expression reagents and a 10x Chromium Controller instrument according to the manufacturer's instructions. A spike-in of 1% PhiX control library (Illumina) was used as the technical control.