GSM9286819: AL1_S31_L001; Mus musculus; RNA-Seq
Tissues stored in RNA later were homogenized using a handheld microtissue homogenizer. Total RNA was extracted using the RNeasy Mini Kit (Qiagen) per manufacturer's instructions. RNA quality and quantity were assessed using a Nanodrop One spectrophotometer and Agilent Bioanalyzer. Batches of RNA were shipped to Genomics Technology core at university of missouri for poly(A) RNA sequencing. cDNA library was prepared followed by Illumina TrueSeq-standard sample preparation protocol.