GSM9555216: THP-1-Rv-2; Homo sapiens; RNA-Seq
Total RNA was extracted using the BeyoZol Animal Tissue Total RNA Extraction Kit (magnetic bead method) following the manufacturer's instructions. RNA integrity was assessed by calculating the RIN number using an Agilent 5300 system. Qualified total RNA was further purified using the RNAClean XP Kit (Cat# A63987, Beckman Coulter, Inc., Brea, CA, USA) and the RNase-Free DNase Set (Cat# 79254, QIAGEN, Hilden, Germany). Total RNA was extracted using the BeyoZol Animal Tissue Total RNA Extraction Kit (magnetic bead method). Ribosomal RNA was depleted from total RNA using the HieffNGS® MaxUp Human rRNA Depletion Kit (YEASen, Cat#12257ES24). Sequencing libraries were then constructed using the Dual-mode mRNA Library Prep Kit (YEASen, Cat#12310ES96) following the manufacturer's instructions. The process included RNA fragmentation, first-strand and second-strand cDNA synthesis, end repair, A-tailing, adapter ligation, and PCR enrichment. Library quality was assessed using the Qubit® 2.0 Fluorometer and Agilent 4200 TapeStation.