Equalizer wastewater sample 1A
Total DNA was extracted using DNeasy PowerSoil Kit (QIAGEN) following the manufacturers protocol. Library preparation and sequencing were done by Biokart Pvt. Ltd., Bangalore, India. The DNA fragmentation was done using the KAPPA fragmentation technique, which resulted in DNA fragments of 600 bp in length. The fragmented samples underwent treatment with Hypa peep plus ERAT enzyme mix for end repair and A-tailing, followed by adapter ligation using DNA ligase. The libraries underwent purification with Ampure beads and quantification using the Qubit dsDNA High Sensitivity test kit (Sigma). Sequencing was completed on an Illumina Hiseq 4000 platform.  The product size was chosen for sequencing based on 0.7x (> 450 bp), which resulted in 250 bp paired end reads.