DNA-Seq of metagenome: surface water in PEI: 2020-10-Site2
Within 24 hours of collection, water samples were sequentially filtered through a 1 m glass fiber pre-filter to remove particulate matter, followed by a 0.22 m membrane filter (Sartorius Stedim Biotech, Germany) to capture microbial biomass. DNA was extracted from the filters using the DNeasy PowerWater Kit. Next Generation sequencing (NGS) libraries were generated from the eDNA using the Nextera XT DNA Library Preparation Kit (Illumina, USA), and sequenced on the Illumina MiSeq platform.