ScRNA-Seq of mus musculus:Slc25a2_KO_HCC
Determine the input cell number based on the expected target cell yield, construct GEMs for single-cell partitioning, and, after confirming normal GEM formation, collect the GEMs and perform reverse transcription with barcoding on a PCR machine. Lyse the GEMs to break the emulsion, purify and enrich first-strand cDNA with magnetic beads, then amplify and quality-check the cDNA. Use the qualified cDNA to prepare a next-generation sequencing library via fragmentation, adapter ligation, sample-index PCR, and final quantitative quality control of the library.