Rhodococcus pyridinivorans FL1
Genomic DNAwas sequenced using a combination of PacBio Sequel IIe and Illumina sequencing Genomic DNAwas sequenced using a combination of PacBio Sequel IIe and Illumina sequencing platforms. For Illumina sequencing, genomic DNA was used for each strain in sequencing library construction. DNA samples were sheared into 400-500 bp fragments using a Covaris M220 Focused Acoustic Shearer following manufactures protocol. Illumina sequencing libraries were prepared from the sheared fragments using the NEXTFLEX Rapid DNA-Seq Kit. Briefly, 5 prime ends were first end-repaired and phosphorylated. Next, the 3 ends were A-tailed and ligated to sequencing adapters. The third step is to enrich the adapters-ligated products using PCR. The prepared libraries then were used for paired-end Illumina sequencing (2  150 bp) on Illumina Novaseq 6000