GSM9286741: NCI-H460 cells, siCYP4F11; Homo sapiens; RNA-Seq
RNA was harvested using trizol (invitrogen) and was cleaned by RNA clean & concentrator kit (Zymo). 500 ng of total RNA was used for the construction of sequencing libraries RNA was purified using poly-T-oligo-attached megnetic beads, followed by dUTP based strand specific cDNA synthesis and directional library prepartion involving fragmentation, adapter ligation, and USER enzyme digestion. The final library was quatified by Qubit and qPCR and assessed for fragment size using a bioanalyzer.