RNA-Seq of P. vannamei
A shrimp sample (designated X-Br) collected in Brazil in 2018 was submitted to the Aquaculture Pathology Laboratory (APL), University of Arizona, for pathogen screening. The sample tested negative for all known viral, bacterial, and fungal pathogens of cultured shrimp. Total RNA extracted from this specimen was subsequently sent to OmegaBioservices (Norcross, GA, USA) for high-throughput sequencing. Library preparation was performed using the TruSeq Stranded Total RNA Library Prep Kit (Illumina, San Diego, CA, USA), and sequencing was conducted on an Illumina HiSeq 2500 platform using a paired-end 2  150 bp configuration. The data was analyzed as previously described.