16S sequencing (V3V4 hypervariable region) of Myotis austroriparius gut microbiome
16S rRNA libraries were prepared using the Quick-16S Library Prep Kit (Zymo Research, Irvine, CA, USA) according to the manufacturers protocol for low microbial DNA samples. Confirmed libraries were pooled according the Quick-16S protocol and the final pooled library was quantified using both a Qubit fluorometer (Thermo Fisher Scientific, Wal-tham, MA, USA) and TapeStation analysis. Libraries were sequenced on the MiSeq platform (Illumina, San Diego, CA, USA) using the MiSeq Reagent Kit v3 (paired-end, 2 x 300 bp).