GSM8986993: IP; Mus musculus; RNA-Seq
RIP experiment was conducted according to millipore RIP kit construction.Total RNA was harvested using Trizol (Invitrogen). Total RNA was used for the construction of sequencing libraries. The RNA fragment size distribution and concentration of the immunoprecipitated (IP) products were analyzed using an Agilent 2100 Bioanalyzer (Agilent Technologies) and a simpleNano spectrophotometer (GE Healthcare). Subsequently, the IP and Input libraries were constructed with the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (New England Biolabs, E7770). Library quality was verified on the Agilent 2100 Bioanalyzer. Paired-end sequencing (PE150) was performed on the Illumina NovaSeq platform.