GSM8817251: MouseBrain_2; Mus musculus; RNA-Seq
Phenol-chloroform RNA extraction was performed for M. musculus, S. cerevisiae and S. pombe, whereas no RNA extraction was needed for the Biochain-purchased RNA for G. gallus and M. mulatta. TGIRT-Seq libraries were constructed as described previously (Boivin et al., RNA, 2018) using ribodepleted total RNA (S. cerevisiae and M. musculus) or gel-purified and size-selected small RNAs (S. pombe, M. mulatta and G. gallus).