Ambler(Native pH:5.14), Time:T9, Sample type:Slurry, Chloramphenicol:None, Cycloheximide:None, Perturbation Unit:-15, Replicate:1
DNA Library preparation was performed using the 16S Metagenomic Sequencing Library Preparation protocol with a 2-stage PCR workflow (Illumina, San Diego, CA, United States). The V3V4 region was amplified using forward primer 341-b-S-17 (CCTACGGGNGGCWGCAG) and reverse primer 785-a-A-21 (GACTACHVGGGTATCTAATCC).