GSM8192544: RNAseq_A375; Homo sapiens; RNA-Seq
Cells were lyzed directly on culture dish with TRIzol RNA isolation reagent (Thermo fisher scientific). Total RNA was extracted by thorough mixing with chloroform and isopropanol precipitation of upper aqueous phase. 500 ng of total RNA was used for poly-A selection of RNA and library construction using TruSeq stranded mRNA LT Kit (Illumina, RS-122-2102). Samples were barcoded and run on a HiSeq 4000 in PE50 to generate 40-50 M reads.