bacterial 16S rRNA gene V1-V9 amplicon-based microbiome profile of sterile paper points
Six sterile paper points were taken as a mock sample. RNA and DNA were simultaneously isolated from each sample using a custom protocol. Bacterial full-length 16S rDNA genes were amplified using the bacteria-specific primer pair 27F (AGRGTTYGATYMTGGCTCAG) and 1492R (RGYTACCTTGTTACGACTT). Preparation for sequencing, including ligation of barcoded SMRTbell Adapters, followed PacBio protocols.