GSM8675679: Atria, CUG960 on/off dox, biol rep 2; Mus musculus; RNA-Seq
Protocol from Rnwasy Fibrous Tissue Mini Kit (QIAGEN) The Genomic and RNA Profiling Core (GARP) at BCM conducted sample quality checks using the NanoDrop spectrophotometer and Agilent Bioanalyzer 2100. The samples passing the following criteria were used for RNA-Seq: RNA integrity number (RIN) ≥ 8.0, A260nm/A280nm ≥ 1.9, A260nm/A230nm ≥ 1, and r28S/16S ≥ 1.5. The Illumina TruSeq Stranded mRNA library preparation protocol was then used to generate cDNA libraries starting with 250 ng of total RNA. The resulting libraries were quantitated using the NanoDrop spectrophotometer and fragment size assessed with the Agilent Bioanalyzer. The pooled libraries were loaded onto a NovaSeq 6000 S4 flow cell and sequenced to a depth of approximately 100 million read pairs/sample. A paired-end 150 cycle run was used to sequence the flowcell on a NovaSeq 6000 sequencing system.