GSM8563964: Oct1 deficient mESC HBO1 ChIP_1; Mus musculus; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 min and quenced with 0.125M glycine. The cells are then lysed with Farnham and RIPA buffer and sonicated with EpiShear probe sonicator. ChIP-seq libraries were prepared using NEBNext Ultra II DNA Library Prep Kit (cat#E7634L) complemented with NEBi5 DNA index. 2ng of DNA was used as starting material for input, and the libraries were amplified for 10 cycles on thermocycler.  PCR-amplified libraries were qualified on an Agilent Technologies 4150 TapeStation using a D1000 ScreenTape assay (cat# 5067-5582 and 5067-5583) and the molarity of adapter-modified molecules was defined by quantitative PCR using the Kapa Biosystems Kapa Library Quant Kit (cat#KK4824).  Libraries were normalized and pooled in preparation for Illumina sequencing.