16S rRNA gene sequencing of Populus tremuloides bark DNA: sample PNL-EC
Libraries for sequencing were prepared according to Illuminas 16S Metagenomic Sequencing Library Preparation guide, with the exception of using Qiagen HotStar Taq DNA polymerase for the first PCR (amplicon PCR). A mixture of peptide nucleotide acid (PNA) blockers oligos (PNA Bio Inc) was also included in the reaction to inhibit plant material amplification. The template specific primers were : 515F-5 (5- GTGYCAGCMGCCGCGGTAA-3) and 926R (5- CCGYCAATTYMTTTRAGTTT-3).