Whole Genome Sequencing of Rhizoctonia solani infecting Proso Millet
The genomic DNA of the isolate was extracted using CTAB method and confirmed as Rhizoctonia solani using internal transcribed spacer (ITS1/ITS4) primers. Library preparation was carried out using TruSeq DNA Nano LP kit. Insert size of libraries were queried on Tapestation 4150 (Agilent) utilizing high sensitive D1000 screen tapes. Passed library was further subjected to whole genome shotgun sequencing using illumina Novaseq 6000 producing paired end (150 x 2) reads.