GSM8988455: mouse tumoroid F11-8 µM Regorafenib; Mus musculus; RNA-Seq
Total RNA from organoids was extracted using Rneasy mini kit (Qiagen) for crypts and cell lines following manufacturer's instructions. RNA libraries were prepared for sequencing using Ovation® SoLo RNA-Seq Library Preparation Kit (NuGEN, TECAN genomics). RNA-Seq 2x100 bases Paired-End