GSM9553540: Distal tumor tissue-1; Homo sapiens; RNA-Seq
Total RNA was extracted with Trizol reagent (Invitrogen, Grand Island, NY) and subsequently purified using the Qiagen RNeasy Mini Kit (Qiagen, Valencia, CA). The integrity of each RNA sample was verified on an Agilent BioAnalyzer (Agilent Technologies, Inc., Santa Clara, CA, USA); only those with an RNA Integrity Number (RIN) exceeding 7.0 were carried forward for library preparation. Library preparation was conducted using the TruSeq RNA Sample Preparation Kit (Illumina), which facilitated mRNA purification, fragmentation, and subsequent double-stranded cDNA synthesis. The completed libraries were quantified with a Qubit fluorometer (Invitrogen, CA, USA), pooled at equimolar concentrations, and finally sequenced on an Illumina NovaSeq X Plus platform.