GSM8817802: neurons,mut,rep2; Homo sapiens; RNA-Seq
Total RNA was extracted using the Trizol-Chloroform method, treated with DNase (Promega #M6101, US), and cleaned up using RNeasy Plus Mini kit (Qiagen Valencia, CA). We synthesized cDNA using ProtoScript II Reverse Transcriptase (New England BioLab, US) and performed RT-qPCR using Applied Biosystems™ Power SYBR™ Green PCR Master Mix (Thermo Fisher Scientific, US) on an Applied Biosystems 7500 Instrument. Libraries for MiSeq were prepared by Massachusetts General Hospital, Center for Computational and Integrative Biology