GSM8783576: N2-3; Caenorhabditis elegans; RNA-Seq
RNA extraction was performed as previously described (Jeong et al., 2020) with minor modifications. Synchronized animals at day 1 were harvested with S-basal buffer with 0.01% polyethylene glycol. Total RNA was extracted using RNAiso Plus and cDNA was obtained using ImProm-II Reverse Transcriptase (A3802, Promega, Madison, WI, USA) with hexamer random primers. Libraries were constructed with TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, CA, USA) by Macrogen Inc. (Seoul, South Korea) following the manufacture's instruction.