GSM8885081: meRIP Input rep2; Mus musculus; OTHER
Total RNAs extracted from the BMDCs were purified with PolyATract mRNA Isolation System. Chemically fragmented RNA (100 nucleotides) was incubated with an m6A antibody for immunoprecipitation by using the Magna methylated RNA immune precipitation (MeRIP) m'A Kit. The obtained m6A-containing mRNA was analyzed by high-throughput sequencing. For high-throughput sequencing, the purified RNA fragments were applied for library construction using the TIANSeq Fast RNA Library Kit for Illumina (TIANGEN). The resulting libraries were sequenced on a Novaseq 6000 platform with paired-end 150-bp read length. The library preparation and high-throughput sequencing were conducted by TIANGEN (Beijing, China). Low-quality reads were removed by Trimmomatic software, and rRNA reads were filtered by Bowtie2 software.