RNA-seq
RNA was obtained from cortices of ten control and ten dcKO embryos at E15.5. Cortices were dissociated and nuclei were stained intranuclear using Tbr2 antibody (rabbit pAb 1:200; Abcam) (Sakib et al., 2021; Ulmke et al., 2021). Nuclei were sorted using FACS. Sorted nuclei were collected in tubes pre-coated to minimize non-specific binding, then pelleted by brief centrifugation. RNA using TRIzol LS (Invitrogen) protocol, followed by aqueous phase purification with the Zymo RNA Clean & Concentrator-5 kit. RNA-seq. libraries were generated using 1 ng of total RNA and the Takara SMART-Seq v4 Ultra Low Input RNA kit, following the manufacturers instructions. Libraries were split into four technical replicates and a paired-end Illumina sequencing was performed on the barcoded libraries using an Illumina NextSeq1000.