GSM9046187: ChIP-seq_sgHBG1_2; Homo sapiens; ChIP-Seq
Cells were fiex with 1% formaldehyde for 10min\ at RT with shaking.After quenching and cell lysis chromatin was sonicated. For ChIP-seq analysis using the Illumina NovaSeq, 10-20 ng of ChIP DNA was processed for library generation using the TransNGS DNA Library Prep Kit according to the manufacturer's protocol(TransGen). Raw reads that aligned to exactly one location in the reference human genome (hg38) were retained for downstream data analysis.