GSM7373372: Sepsis 17,Human, Blood; Homo sapiens; RNA-Seq
Total RNA was isolated from whole blood using the TRIzol reagent (Invitrogen, USA). Messenger RNA (mRNA) was isolated using a Poly(A) RNA Selection Kit (Lexogen, Inc., Vienna, Austria). Libraries were prepared from total RNA using a NEBNext Ultra II Directional RNA-Seq Kit (New England BioLabs, UK).