scRNAseq of mus musculus: adult female spleen
Cells were sorted on a BD Bioscience FACSAria III Fusion sorter, and the Chromium Next GEM Single Cell V(D)J Reagent Kits v1.1 were used to process the single-cell solutions. The scBCR libraries were sequenced on the Illumina MiSeq platform. The sequencing data were analyzed with the Loupe V(D)J Browser, and productive sequences were re-annotated with IMGT/HighV-Quest.