GSM8563674: MDA-MB-231 cells, VIM-/-, rep3; Homo sapiens; RNA-Seq
Total RNA was isolated using the Quick-RNA™ Miniprep Kit.RNA Purity and quantification were checked using SimpliNanoTM - Biochrom Spectrophotometers (Biochrom, MA, USA). RNA degradation and integrity were monitored by Qsep 100 DNA/RNA Analyzer (BiOptic Inc., Taiwan). A total amount of 1 μg total RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using KAPA mRNA HyperPrep Kit (KAPA Biosystems, Roche, Basel, Switzerland) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.