GSM8565045: HAP1-SD2-Rep2; Homo sapiens; OTHER
gDNA was extracted using the QIAamp DNA Blood Maxi kit (Qiagen #51194). Next generation sequencing of sgRNAs was carried out following PCR amplification of pooled sgRNAs to incorporate Illumina-based adaptors for 75 bp single end sequencing carried out on an Illumina sequencer