GSM8708284: mouse poly-A seq Liver WT 1646; Mus musculus; RNA-Seq
Tissues were extracted and frozen on dry ice. A bead mill (Retsch, Düsseldorf, Germany) was used to pulverize the tissue. RNA was subsequently extracted from the tissue powder using PeqGold TRIFAST, a phenol-based extraction protocol, (VWR, Germany) according to the manufacturer's protocol. RNA integrity was visualized using a Bioanalyzer (Agilent, Santa Clara, CA, USA) Each sample was prepared for library construction separately. The 60 libraries were prepared using the (30x) Illumina stranded mRNA Prep and (30x) Illumina stranded total RNA Prep (Illumina, San Diego, CA, USA) according to the manufacturer's recommendations