Whole genome sequencing Triticum durum Mahmoudi
High-molecular-weight (HMW) genomic DNA was extracted from isolated nuclei using the PacBio Nanobind Plant Nuclei Kit (PacBio, 102-207-900), following the manufacturers instructions. DNA quantity and integrity were assessed prior to library preparation. Extracted HMW DNA was used for both long-read (PacBio) and short-read (Illumina) sequencing.