GSM9491427: 60 AB zebrafish larvae, Biol rep 1, WT; Danio rerio; RNA-Seq
Total RNA was extracted from 4 days post fertilization zebrafish larvae using TRIzol/chloroform and isolated through a series of isopropanol and ethanol washes. 60 larvae were used per RNA sample. Total RNA samples were submitted to the VANTAGE (Vanderbilt Technologies for Advanced Genomics) core for RNA quality assessment and library construction using either the NEBNext rRNA depletion or Illumina Ribo-Zero Plus rRNA depletion kit.