Pseudosorghum zollingeri 90627 (CRX1324707; CRA021780)
Total genomic DNA from newly sampled individuals was extracted from silica-dried leaves collected in the field or, in a few cases, from herbarium specimens, using the modified CTAB method. Purified DNA samples were sheared into 350 bp fragments to construct short-insert libraries for genome-skimming sequencing with 2 x 150 bp reads on a DNBSEQ-T7 platform.