GSM8851006: dis3_3; Caenorhabditis elegans; RNA-Seq
Total RNA was isolated using RNAiso plus (Takara, Japan) in accordance with the manufacturer's instructions. Synchronized prefertile wild-type worms, dis-3(L75Q), eat-2(ad1116), and eat-2(ad1116); dis-3(L75Q) mutants maintained on OP50 at 20°C, were harvested three times independently using M9 buffer containing 0.01% polyethylene glycol (PEG). Total RNA was isolated using RNAiso plus (Takara, Japan) in accordance with the manufacturer's instructions. A sequencing library was constructed and paired-end RNA sequencing using Illumina NovaSeq 6000 platform was performed (Macrogen, South Korea).