GSM9553610: Mouse heart RNA-seq ISO (A3); Mus musculus; RNA-Seq
Total RNA was extracted from mouse heart tissue using TRIzol reagent (Invitrogen) according to the manufacturer's instructions. RNA integrity was assessed using an Agilent Bioanalyzer 2100. mRNA-seq libraries were prepared by Novogene (Batch 1) and Majorbio Bio-Pharm Technology (Batch 2) using a standard poly(A)+ mRNA enrichment protocol with oligo(dT) beads, followed by RNA fragmentation, cDNA synthesis, end repair/A-tailing, adapter ligation, size selection, and PCR amplification. Libraries were sequenced on an Illumina platform as paired-end reads (instrument model/read configuration as reported in the raw sequencing metadata).