The transcriptomes in AKI and LB media
rRNA was depleted using the RiboCop rRNA Depletion Kit for Mixed Bacterial Samples (Lexogen, USA). The resulting mRNA was fragmented (~200 bp) and reverse-transcribed into strand-specific cDNA using random primers with dUTP incorporated during second-strand synthesis. After end repair, A-tailing, and adapter ligation, the dUTP-containing second strand was removed by UNG treatment. Libraries were prepared using the Illumina Stranded mRNA Prep, Ligation kit (Illumina, San Diego, CA), and paired-end sequencing was performed on the Illumina NovaSeq X Plus platform.