GSM9045925: DSP-1001660020631-H-E02, NP-3_005_PanCK+, NanoString GeoMx DSP; Homo sapiens; OTHER
Clinical NPC FFPE samples were sectioned into 5-µm slide tissue sections were fluorescently stained with DNA dye (SYTO 13) and the primary antibodies targeting CD20 (NanoString Technologies, clone ID: IGEL/773), CD45 (NanoString Technologies, clone ID: D9M8I), PanCK (NanoString Technologies, clone ID: AE-1/AE-3), as well as the secondary antibody (Thermofisher Goat Anti-rabbit, A-11012). RNA probes coupled with photocleavable oligonucleotide barcodes were used to bind specifically to target mRNAs  during the incubation process on the DSP platform on the basis of in situ hybridisation. Then, each segment was exposed to UV radiation, detaching the barcodes from the probes/antibodies. The cleaved barcodes in units of segments were then collected into multiwell plates for next-generation sequencing on an Illumina sequencer.