GSM9455088: Wild-type-Aged-A2-F3_S57_L004; Mus musculus; RNA-Seq
RNA from cleaned aortic tissues was isolated using RNeasy Mini Kit (Qiagen cat #217004), as indicated by the manufacturer. RNA quality and RNA sequencing (RNA-seq) were conducted at NYU Langone's Genome Technology Center (RRID: SCR_017929). Aortic tissue from 2 different mice (~20mg) were combined, totalizing 5 samples (n=5). All samples were processed in the same time period, following the same protocol in order to limit the batch effects. Next, the RNASeq enrichment libraries were prepared using the low-input RNA sequencing, TruSeq Stranded RNA Library Prep Gold.