scRNA-seq of control and eIF6-overexpressing MLL-AF9 leukemia stem cells
scRNA-seq of control and eIF6-overexpressing MLL-AF9 leukemia stem cells
100,000 bone marrow cells from terminally sick leukemia-engrafted mice were transplanted into sublethally irradiated (475 rad) recipient mice. Bone marrow cells were harvested from leukemia-engrafted mice that were administered Dox for six days and viable GFP+ leukemia cells were sorted into PBS containing 0.05 % BSA following the manusfacturer's protocol.  For each sample, 15,000 cells were loaded in a 10x Genomics Chromium Next GEM Chip G channel.  Libraries were constructed using 10x Genomics Chromium Next GEM Single Cell 3' v3.1 kit and the quality of sequencing libraries was determined using Agilent 4200 Tapestation system according to manufacturer's instructions.