GSM9045806: Whole blood, healthy control 4; Homo sapiens; RNA-Seq
This workflow includes RNA extraction from PAXgene™ blood RNA tubes, depletion of ribosomal RNA via hybrid capture (Illumina Ribo-Zero), and Illumina TruSeq Stranded Total RNA Library Preparation (input 200-1000 ng of Total RNA). The stranded RNA samples are sequenced on the Illumina NovaSeq 6000 next generation sequencing platform (2 x 150 base pair) for a depth of 50 million paired end reads. The cleaned sequence reads were aligned against the Homo sapiens genome (Build version hg38), and the STAR aligner (v2.5.3a) was used to map unique reads to the genomic sequences14. FASTQ files were processed into gene and transcript counts