GSM8534713: RNA-seq-H9_DOT1L_KO-D19; Homo sapiens; RNA-Seq
After the total RNA extracts were prepared, RNA libraries were established using VAHTS® Universal V8 RNA-seq Library Prep Kit for Illumina (Vazyme) according to the manufacturer's protocol 1000 ng of total high-quality RNA (RIN value >7) was used to ensure sufficient mRNA for library construction. mRNA was enriched by the Poly(A) method with VAHTS mRNA Capture Beads (Vazyme #N401)