GSM9195397: KDM5B ChIP in S12N:SD2(deadSET) cells, replicate 2; Mus musculus; ChIP-Seq
~25 million cells per genotype were double-crosslinked in 2 mM disuccinimidyl glutarate (DSG) for 30 minutes then  1% formaldehyde for 10 minutes. Crosslinked cells were collected off the plates and snap-frozen as pellets. Cells were lysated in standard buffers and sonicated using Covaris E220 acquistic sonicator following manufacturer's recommendations. ChIP-seq libraries were prepared using NEBNext® Ultra™️ II DNA Library Prep Kit for Illumina® (NEB #7645) and adaptor and multiplex oligos from NEBNext® Multiplex Oligos for Illumina® (E6442L).