GSM9337177: Myc_ON_#6; Mus musculus; RNA-Seq
Lungs were isolated from KM mice, placed into MACS tissue storage solution (130-100-008, Miltenyi) on ice and processed to single cell suspension on a gentleMACS Octo Dissociator with Heaters (130-096-427, Miltenyi) according to manufacturers' instructions.  In brief, after manually dissecting the lungs in ~1mm2 pieces, they were further processed into a single cell suspension using the mouse tumour dissociation kit (130-096-730, Miltenyi) in gentleMACS C tubes (130-096-334, Miltenyi). After dissociation the cell suspension was incubated with Red Blood Lysis Solution (130-094-183, Miltenyi) to remove red blood cells. Subsequently the cell suspension was filtered through 70µm MACS SmartStrainers (130-098-462) before being counted. 6000 cells per sample were offered for single cell sequencing. Library preparation of murine samples was performed according to instruction in the 10X Chromium single cell kit 3' v3.