GSM9518104: THP1 treated with 30Ab, replicate 3 (Batch 4427); Homo sapiens; RNA-Seq
For each sample, 500 ng of total RNA was used as the starting material. Following the Illumina Stranded mRNA Sample Prep protocol, poly-A containing mRNA molecules were purified from the total RNA input using poly-dT oligo-attached magnetic beads. Purified dual-indexed sequence-libraries of each sample were equimolarly pooled,  converted with the Adept PCR Plus kit (Element Biosciences) and sequenced on  Element Biosciences AVITI (two runs, 2x75 Cloudbreak High kit, single read 100 (10110-10-0))