Oxford Nanopore sequencing of P. faecium AB13 from human mucosal-luminal interface aspirates
Single colony grown in 104c PY+X+80mM disodium succinate, anaerobically at 37C for 4 days. DNA extracted using Quick-DNA HMW MagBead Kits, with an additional RNase A treatment and two additional g-DNA Wash Buffer washes. Oxford Nanopore long-read sequencing using Rapid Sequencing DNA V14 kits (SQK-RBK114.24) on a MinION Mk1C with R10.4.1 flow cells (FLO-MIN114).