GSM8381560: Control, replicant 2 [miRNA]; Oryctolagus cuniculus; miRNA-Seq
Briefly, total RNA was extracted from samples with an achieved yield of 1 µg per sample. Integrity and purity were confirmed using agarose gel electrophoresis (28S:18S ≥ 1.5) and Nanodrop spectrophotometry (OD260/280 ratio between 1.8 and 2.2), respectively. RNA concentration was quantified using a Qubit 4 Fluorometer (Thermo Fisher Scientific, Inc.), with minimum readings of 500 ng/µl. Adaptors were added to both ends of small RNAs, and cDNA was synthesized through reverse transcription, followed by purificatiox n using QIAseq  miRNA NGS Beads (cat. no., 333923; Qiagen).