A_3 (CRX1643557; CRA025122)
The sorted CD45- cells suspension was loaded into Chromium microfluidic chips with 3' v3 chemistry and barcoded with a 10x Chromium Controller. RNA from the barcoded cells was subsequently reverse-transcribed and sequencing libraries constructed with reagents from a Chromium Single Cell 3' v3 reagent kit. Sequencing was performed with Illumina Novaseq X plus.