GSM9554137: C-2; Mus musculus; RNA-Seq
Total RNA was extracted using the TRIzol reagent (Invitrogen, CA, USA) according to the manufacturer's protocol. RNA purity and quantification were evaluated using the NanoDrop 2000 spectrophotometer (Thermo Scientific, USA). First, use the Single Cell Full Length mRNA-Amplification Kit to reverse transcribe and amplify RNA. For the cDNA that passes the quality inspection, use the cDNATruePrep DNA Library Prep Kit V2 for Illumina to construct the library in accordance with the instructions.