Human gut metagenome from MIAB sample 99, meta-HiC proximity ligation
Human gut metagenomic meta-HiC library generated using Phase Genomics ProxiMeta kit v2.0. Intact cells were crosslinked with formaldehyde, digested using Sau3AI and MlucI restriction enzymes, and proximity ligated with biotinylated nucleotides to create chimeric molecules from physically proximal genomic regions. DNA with meta-HiC junctions was pulled down with streptavidin beads. Sequenced as paired-end 151 bp reads by Phase Inc.