GSM9631368: NTU3; Mus musculus; RNA-Seq
After lysing the cells with TRIzol (Accurate Biology, AG21102), RNA was purified using an RNA extraction kit (accurate biology, AG21017) Purified mRNA was fragmented into short fragments using Fragmentation Buffer. The fragmented RNA was then reverse-transcribed with random primers to synthesize first-strand cDNA, followed by second-strand cDNA synthesis using 2nd Strand Marking Buffer and 2nd Strand/End Repair Enzyme Mix. The double-stranded cDNA subsequently underwent end repair, A-base addition, and sequencing adapter ligation. After magnetic bead-based size selection, the target fragments were recovered and amplified by PCR to complete library construction.