RNA-seq profiling of MED patient-derived (MATN3 T195K) and unaffected iPSC-chondrogenesis
RNA-seq profiling of MED patient-derived (MATN3 T195K) and unaffected iPSC-chondrogenesis
Cells harvested into QIAGEN RLT buffer. Pellets homogenized with molecular grinding resin and plastic pestle. hESC/hiPSC cultured in TeSR™-E8™ on vitronectin. Differentiation to iMSCs via MesenPRO RS™. 3D pellets (200k cells) cultured in medium with TGFβ3 and BMP2. RNA extracted using QIAGEN RNeasy mini kit, as per manufacturers instructions. mRNA libraries were prepared using the Illumina stranded mRNA prep kit.