Illumina sequencing of Oropouche isolate UTMB
RNA samples were quantified using a Qubit 4.0 Fluorometer and RNA integrity was checked using RNA Kit on Agilent 5300 Fragment Analyzer (Agilent Technologies). rRNA was depleted using a FastSelect rRNA HMR kit (Qiagen), and RNA sequencing prepared using a NEBNext Ultra RNA Library Kit for Illumina (NEB). The sequencing libraries were then validated using NGS Kit on an Agilent High Sensitivity D1000 ScreenTape (Agilent Technologies) and following this quantified using a Qubit 4.0 Fluorometer (Invitrogen). Libraries were sequenced on an Illumina NovaSeq X using a 2x150 pair-end configuration.