MinION sequencing of SAMD01795638
Genomic DNA was mechanically sheared to 10~15kb using Covaris g-TUBEs. Libraries were prepared using the Oxford Nanopore Technologies (ONT) Ligation Sequencing Kit with Native Barcoding 24 V14 (SQK-NBD114.24) and R10.4.1 flow cells (FLO-MIN114). Basecalling was performed using MinION Mk1B via Dorado (SUP or HAC mode specified per run).