GSM8815990: Tumor organoid without treatment; Homo sapiens; RNA-Seq
To generate MRTX1133-resistant patient-derived organoids, SNU-4646S1-TO cells were treated with MRTX1133. The half-maximal inhibitory concentration (IC50) of MRTX1133 for each model was calculated using the CellTiter-Glo assay (Promega, Madison, WI, USA). After MRTX1133 treatment, surviving cells were cultured in drug-free medium for 3 days before further treatment. The dosages used in this procedure were gradually increased from IC6.25 to IC50. After 6 months, the IC50 values of MRTX1133 in the resistant cell lines exceeded 1 mM. For the parental organoids, SNU-4646S1-TO cells were cultured for 6 months without MRTX1133 treatment. Normal organoids, SNU-4646S1-NO, were directly used for library extraction. Library was constructed using Chromium Next GEM Single Cell 3' Reagent Kits version3.1 (10x Genomics)