Single-nucleus libraries were prepared from snap frozen samples using the 10x Genomics Chromium Fixed RNA Profiling platform following manufacturer instructions (Pleasanton, CA, USA). Input targets were approximately 2,000–10,000 nuclei per sample where possible, with a sequencing depth target of ~50,000 reads per nucleus. Libraries were sequenced on Illumina platforms to a combined depth of approximately 700 million reads across experiments. Samples from distinct subjects were multiplexed (pooled and indexed with unique barcodes) and ran as a single run. One sample within this multiplexed dataset is OCT.