metagenomic of Mus musculus: adult male fecal
The genomic DNA sample was fragmented into short fragments. These DNA fragments were then end-polished, A-tailed, and ligated with full-length adapters for Illumina sequencing before further size selection. PCR amplification was then conducted unless specified as PCR_x0002_free. Purification was then conducted through the AMPure XP system (Beverly). The resulting library was assessed on the Agilent Fragment Analyzer System (Agilent) and quantified to 1.21nM through Qubit (Thermo Fisher Scientific) and qPCR. The qualified libraries were pooled and sequenced on Illumina platforms, according to the effective library concentration and data amount required.