GSM8501115: D73_R1_TRIP_NSC; Homo sapiens; RNA-Seq
Cells were first dissociated in Accutase for 30 minutes at 37 C to generate a single cell suspension. Cells were fixed in 100% methanol following the 10X Genomics established protocol. Library preparation and mRNA barcoding was performed using the Chromium Single Cell 3′ Reagent Kit v3 (10x Genomics), following the manufacturer's instructions. 10,000-cell recovery was the targeted for each sample. One library was prepared for each condition (Clone 1-13 and ND34391G). Libraries were sequenced with NovaSeq 6000 (Illumina).