GSM9632124: BMPDC_WT1_1.fq.gz; Mus musculus; RNA-Seq
For RNA-seq analysis, 2×106 pDCs or BM pDCs were sorted from BM of WT and cKO mice. BM pDCs were centrifuged at 1750 rpm for 5 min at 4°C to remove the supernatant. After resuspending in 1 mL of pre-cooled PBS, the cells were centrifuged again and the supernatant was discarded. To lyse the pDCs, 1 mL of TRNzol RNA extraction reagent was added, and the cells were thoroughly disrupted. RNA for libraries for RNA-seq were prepared using  polyA_selected