GSM9555081: sgLkb1 (Lkb1 Mutant KP Tumor) Rep 3; Mus musculus; RNA-Seq
Mouse lung tissue was digested with Collagenase/DNAse in RPMI +10% FBS at 37 degrees for 35 minutes. Samples were washed and underwent RBC lysis. Samples were washed again and resuspended in FACS buffer, then stained for CD45 prior to sorting.  RNA was extracted usine an Invitrogen PureLink RNA extraction kit. Sequencing libraries were prepared using Nextera XT DNA library preparation kit (Illumina) per manufacturer's instruction