RNA-Seq of Prunus avium leaf infected by Alternaria alternata
Total RNA was extracted by Trizol method entrusted to the above company. After extraction, the RNA samples were quickly frozen in liquid nitrogen and stored at -80 for transcriptome analysis. RNA quality was verified by agarose gel electrophoresis (to detect integrity and DNA contamination), Qubit 4.0 Fluorometer/MD Microplate Reader (to quantify concentration), and Qsep400 Bioanalyzer (to detect integrity). After passing the quality inspection, library preparation was completed through mRNA isolation, cDNA synthesis and a series of library construction steps.Library quality control: Preliminary quantification was performed by Qubit dye method, and the insert size was detected by a Fragment Analyzer. After qualification, accurate quantification was conducted by Q-PCR (effective concentration > 2nM). Subsequently, DNBs (DNA Nano Balls) were prepared and loaded onto sequencing chips, and high-throughput sequencer was used to sequence 18 samples. All the above sequencing-related operations were entrusted to the above company.