GSM9486124: Wild-type S. aureus, iron-replete, biol rep 1; Staphylococcus aureus; RNA-Seq
Cells were normalized to an OD600 equivalent of 3.0, pelleted, lysed, and RNA extraction performed with Trizol (Invitrogen) according to manufacturer's instructions. Extracted RNA was then subject to rigorous DNAse treatment (Invitrogen) and phenol chloroform extraction to inactivate and remove DNAse. RNA samples were processed by the Microbial Genome Sequencing Center in Pittsburgh, PA. Library preparation was performed using Illumina's Stranded Total RNA Prep Ligation with Ribo-Zero Plus kit and 10bp unique dual indices (UDI). Sequencing was done on a NovaSeq X Plus, producing paired end 150bp reads.