MC38 tumor sample2 treated with PD-1 antibody for RNA-seq (CRX1712841; CRA025527)
To perform transcriptome sequencing on tumor tissue samples, we extracted total RNA using TRIzol reagent according to the manufacturer's instructions. The quality and quantity of RNA were assessed using an Agilent Bioanalyzer 2100 system. For library preparation, we used the TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold according to the manufacturer's instructions. Briefly, poly(A) mRNAs were purified using oligo-dT magnetic beads. RNA samples were then fragmented, and cDNA was synthesized using primers containing unique indexes.