GSM9629949: HFD_ONX3; Mus musculus; RNA-Seq
Total RNA was extracted from liver tissues using a TRIzol-based method according to the manufacturer's instructions. RNA concentration and integrity were assessed prior to library preparation. RNA-seq libraries were prepared following standard Illumina library preparation protocols. Briefly, mRNA was enriched, fragmented, reverse-transcribed into cDNA, ligated with sequencing adapters, PCR amplified, and subjected to quality control prior to sequencing.