FFPE DNA underwent an enzymatic process to repair some of the DNA damage that results from fixation and storage. 100-200ng of DNA was then fragmented under mild settings using a low volume AFA plate on the Covaris LE220 to target 500bp fragments in accordance with manufacturer's instructions. Fragmented samples were then processed with the KAPA Hyper Library Preparation kit (Roche) following the modifications for degraded samples. WGS was performed on the NovaSeq 6000 instrument (Illumina) using the v1.5 S4 300-cycle reagent kit (2x150 bp read length) to a targeted mean coverage of 30X.