GSM9594075: Chicken control liver 1; Gallus gallus; RNA-Seq
Total RNA was extracted using RiboPure™ RNA Purification Kit (Invitrogen™). The samples further processed for rRNA depletion to enrich host  mRNA using using RiboMinus Eukaryote Kit v2 (Invitrogen) and the enriched mRNA fraction was concentrated using the RiboMinus Concentration Module (Invitrogen). All steps were carried out according to the manufacturer's instructions. Quality and concentration of RNA samples in each step was measured using NanoDrop™ 2000/2000c Spectrophotometer (Thermo Scientific™). Library preparation was done using TruSeq® Stranded mRNA library kit (Illumina Inc., San Diego, CA, USA) as per manufacturer's recommendations