RNA-seq of Coptodon imbrifernus adult female retina
Total RNA was extracted from tissue samples using the RNeasy Mini Kit (Qiagen) following the manufacturers protocol. RNA quality and concentration were assessed using a Bioanalyzer (Agilent Technologies). Strand-specific RNA-seq libraries were prepared using the Illumina TruSeq Stranded mRNA Library Prep Kit according to the standard protocol. Libraries were then sequenced on an Illumina platform to generate 150 bp paired-end reads.