RNA-Seq of rat liver: adult male liver
Total RNA was extracted from Sprague Dawley rat liver tissue using the RNeasy Mini Kit (Qiagen). Poly(A)+ mRNA was subsequently enriched using oligo(dT) beads. RNA quality control was performed as follows: concentration and purity (10 ng/l, total amount 500 ng, A260/280 1.5) were measured on a NanoDrop spectrophotometer (ThermoFisher); RNA integrity (RQN 7) was assessed using the Qsep-100 system (Bioptic). All submitted samples passed these quality thresholds, and clear ribosomal bands were observed via gel electrophoresis, confirming high RNA integrity.