ITS2 of Soil
Sequencing  fungal communities of soil (MP16 treated and non-treated) were prepared according to the Illumina ITS2 Metagenomic Sequencing Library Preparation guide. For Fungal communities, the ITS2 region were amplified using ITS3 primers (For: 5- GCATCGATGAAGAACGCAGC-3and IT4 Rev5-TCCTCCGCTTATTGATATGC-3) . Sample-specific 6-bp barcodes were incorporated into the primers for multiplex sequencing. The PCR reaction was done using the Phusion High-Fidelity PCR Master Mix (NEB). The PCR products were verified on 2% agarose gel, for ITS were cut and mixed in an equal-density ratio and  then purified the PCR product with Qiagen Gel Extraction Kit (Qiagen). Following the manufacturer's recommendations, the generated sequencing libraries using NEB Next Ultra DNA Library Prep Kit for Illumina added the index codes. The library quality assisted using the Qubit dsDNA high-sensitivity (HS) kit (Thermo-Fischer Scientific, Schwerte, Germany) and Agilent Bioanalyzer 2100 system. The library was normalized and sequenced on the Illumina platform (Illumina Inc., San Diego, CA, USA) and generated 2x 250 bp paired-end reads.