GSM8675854: HCT116,C1QTNF1-AS1,LNAA,biol repl 3; Homo sapiens; RNA-Seq
RNA was extracted from HCT116 cells 48hrs after transfection using RNeasy® mini kit (74106, QIAGEN) and treated with DNase I (79254, QIAGEN) according to the manufacturer's instructions. RNA quality was analysed on the Tapestation (Agilent) before proceeding with the library preparation. 1ug of RNA was used for library preparation. RNA-seq libraries were prepared from HCT116 cells using the CORALL™ Total RNA-Seq V2 Library Prep Kit with UDIs set B1 (184.96, Lexogen) with Lexogen RiboCop rRNA Depletion Kit for Human/Mouse/Rat V2 following the workflow for generation of long insert sizes.