RNA-Seq of Saccharina latissima: sporohyte
Isolation of RNA from extractions was achieved by removing DNA using the TURBO DNA-free Kit (Invitrogen). Extracted RNA quality and quantity was checked using a Qubit RNA HS kit and Agilent RNA HS kit on a 4200 TapeStation System. RNA libraries were constructed using the NEBNext Ultra II RNA RNA Library Prep Kit for Illumina (New England Biolabs) and quality checked using the Agilent 4200 TapeStation System. Paired-end sequencing (2x75bp) of transcriptomes was performed on an Illumina HiSeq4000, giving ca. 30 million reads per sample.