GSM8986563: CD45- WT, GEX; Mus musculus; RNA-Seq
Lungs were minced and digested, and flow cytometric sorting (BD FACSAria II) was used to CD45.2– structural cells from WT and Plaur–/– mice. Approximately 16,000 live cells at or above 4×10^5 live cells/mL with 70% or higher viability were loaded into the Single Cell Chip followed by forming 10,000 single cell emulsion in Chromium Controller (10x Genomics). The first strand of gene derived transcripts is synthesized according to manufacturer's instructions (10x Genomics, catalogue #PN-1000268The Gene Expression Libraries were prepared following manufacturer's instructions. The libraries were sequenced by the NIEHS Epigenomics and DNA Sequencing Core Laboratory on NovaSeq 6000 (Illumina) with paired-end sequencing (Read1: 28;  Read2: 90). A total of 228,525,028 reads were obtained.