16S of human tongue lesion (FFPE): Low Malignant Potential
Formalinfixed paraffin-embedded (FFPE) tissue of tongue lesions was retrieved from archived biopsy specimens. Genomic data was extracted using the FFPE Tissue kit (Qiagen, Valencia, CA, USA). Paraffin was removed using Xylene and ethanol washes prior to proteinase K digestion. Targeted amplification of the 16S rRNA gene in the V3-V4 hypervariable regions was performed on the Illumina MiSeq platform.