GSM8534273: C10T; Homo sapiens; RNA-Seq
Single-cell library preparation was performed according to the manual of the Chromium Next GEM Single-cell 3' Reagent Kits v3.1 the Chromium Next GEM Single Cell 5′ Kit v2 for Dual Index (10x Genomics). The cell suspensions were loaded on the Single-cell G Chip for gel bead-in-emulsion (GEM) generation and barcoding. cDNA library thus was generated with a droplet-based sequencing platform (10X Genomics). Next, reverse transcription, RT cleanup cDNA amplification, and quantification was performed to construct the 3' and 5' gene library. The libraries were sequenced on DNBSEQ-G400 (MGI Tech).