GSM8849354: Colorectal cancer tissue, liver metastasis,; Homo sapiens; RNA-Seq
Fresh surgical resection tissues from two human colorectal cancer patients were dissociated into single - cell suspensions. Specific dissociation method not detailed in provided content, could assume using appropriate proteolytic enzymes and mechanical disruption to break down tissue into single cells. Library was performed using the 10x Genomics Chromium platform according to the manufacturer's instructions. The single - cell suspensions were processed to generate gel bead - in - emulsion (GEM), and poly - A RNA from cell lysates in GEMs was retrotranscribed to cDNA, which contains Illumina R1 primer sequence, Unique Molecular Identifier (UMI) and 10x Barcode. The pooled barcoded cDNA was then cleaned up, amplified by PCR and appropriate sized fragments were selected for subsequent library construction. Illumina R2 primer sequence, paired - end constructs with P5 and P7 sequences and a sample index were added during library construction.