GSM8502246: MC38/gp100 Mtap KO 0.5 μM GSK3326595_1; Mus musculus; RNA-Seq
Tumor and T cells were directly lysed using Trizol (Life Technologies, 15596018). RNA for cell lines was isolated using a Direct-Zol RNA miniprep plus kit (R2072, Zymo Research). NEBNext® Ultral II Directional RNA Library Prep with PolyA selection. NovaSeq X plus was used for sequencing.