GSM8381380: subcutaneous tumor, αPD-L1 group, mouse 1; Mus musculus; RNA-Seq
There were four treatment regimen groups: control, TSME only, αPD-L1 only, and αPD-L1 + TSME Tumor tissue samples were processed to enrich mRNA using Oligo(dT) beads. The polyA-tailed mRNA was then fragmented using divalent cations in NEB Fragmentation Buffer. RNA libraries were prepared using the library construction kit NEBNext®Ultra™RNA from Illumina®, following the common protocols of NEB library construction.