GSM8962902: EVTd8_PPARG_KD2_rep1; Homo sapiens; RNA-Seq
Total RNA was extracted from cells using the RNeasy Plus Mini Kit (Qiagen) (74136). The mRNA was enriched using the NEBNext® Poly(A) mRNA Magnetic Isolation Module kit (E7490L). The RNAs-seq libraries were constructed using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina (E7770L) and NEBNext Multiplex Oligos for Illumina (E7600S) following the manufacturer's protocol. RNA-seq was conducted as paired-end 150 bp sequencing on the NovaSeq 6000 (Illumina).