ddRAD-Seq of an F2 Amaranthus tuberculatus population : iw02_sample_69
Tissues from 188 plants of an F2 Amaranthus tuberculatus population were collected prior herbicide treatment. Genomic DNA was extracted following the cetyltrimethylammonium bromide (CTAB) protocol. The double digest restriction-site associated DNA sequencing (ddRAD-Seq) libraries were prepared using the restriction enzymes NsiI-HF and MseI to fragment 255ng of genomic DNA for each individual sample. Samples were sent to the Roy J. Carver Center at the University of Illinois for sequencing on Illumina NovaSeq X Plus 10B to generate 100 bp single-reads.