RNA-seq of Mus musculus: ear-derived fibroblasts, passage 9
Cell lysis, 15-minute Trizol. RNA extracted using phenol-chloroform separation and isopropanol precipitation. RNA treated with DNase 1 (New England Biolabs #M0303S). The University of Wisconsin-Madison Biotechnology Centers Gene Expression Center Core Facility (research resource identifier [RRID]: SCR_017757) conducted quality control assessments. RNA library preparation, sequencing, and read demultiplexing were performed by the University of Wisconsin Biotechnology Center DNA Sequencing Facility (RRID:SCR_017759). The Illumina TruSeq-Stranded mRNA kit was used for polyA+ enrichment for library preparation. Double-stranded cDNA was purified using NEB SPRIselect beads. Library quantification was performed using the PicoGreen assay on an Agilent BioTek Synergy H1 microplate reader, and library quality was assayed on the 4200 Tape Station on a D1000 ScreenTape.