TCR-seq of homo Sapiens
PBMCs were obtained from healthy donors or indicated patients with written informed consents. DNA was extracted from 1  106 normal T cells sorted from PBMCs sample, with the exclusion of CAR T cells and tumor cells, using RNusing GenFIND DNA extraction kit (Agencourt, Beckman Coulter, Brea, CA, USA) following the manufacturers instructions, and subsequently subjected to T cell receptor (TCR) sequencing in  Beijing Boren Hospital (Beijing, China). TCR beta chain CDR3 regions were amplified using multiplex PCR with primers annealing to V and J segments and using the Oncomine TCR Beta-LR Assay and sequenced in Ion Torrent S5 platforms with single end 400bp strategy.