GSM9555773: MET3; Homo sapiens; RNA-Seq
Ten treatment-naïve oral squamous cell carcinoma (OSCC) patients were recruited from the First People's Hospital of Yunnan Province, including 5 primary lesions and 5 lymph node metastases. Samples were obtained from fresh surgical resections. Tissue dissociation was performed using a human tumor dissociation kit (Miltenyi Biotec, Germany) in accordance with the manufacturer's protocol. The dissociated cells were then resuspended, and dead cells were removed using a Dead Cell Removal Kit (Miltenyi Biotec, Germany). Subsequently, the overall cell viability was confirmed to exceed 85%. The cells were further suspended for cell counting and diluted to an appropriate concentration for downstream applications. Single-cell suspensions were loaded onto 10X Chromium chip in order to capture 5000-10,000 single cells, following the manufacturer's instructions of 10X Genomics Chromium Single-Cell 3' kit (V3). The captured single cells underwent cDNA amplification and library construction using the standard protocol of the 3' kit (V3). The single-cell libraries were sequenced on Illumina NovaSeq 6000 sequencing system (paired-end multiplexing run, 150 bp).