single cell RNA-seq of Sham-1 (CRX1109010; CRA017131)
After total RNA was extracted, mRNA was purified from total RNA (5ug) using Dynabeads Oligo (dT) with two rounds of purification. Following purification, the mRNA was fragmented into short fragments using divalent cations under elevated temperature(Magnesium RNA Fragmentation Module at 94 degrees Fahrenheit for 5-7 minutes).