Hic whole-genome sequencing of D. ruficollis
This study employed Hi-C technology to analyze three-dimensional genome architecture. Following formaldehyde crosslinking, chromatin was digested with DpnII restriction enzyme, and the resulting ends were biotin-labeled and subjected to proximity ligation. The purified DNA was fragmented, end-repaired, and ligated to sequencing adapters. Biotin-labeled interaction fragments were specifically enriched using streptavidin beads, followed by PCR amplification to construct the sequencing library.