IPN bucket challenge
IPN bucket challenge
Fish from the bucket challenge were sampled at 15 and 23 days post-challenge and stored in RNALater solution for downstream RNA extraction.  Total RNA was extracted from liver stored in RNALater using RNeasy Mini kit (Qiagen®). A rotor-stator homogenizer (TissueRuptor® II, Qiagen®) was used to disrupt and homogenize the tissue. RNA was peluted according the manual. RNA was eluted in RNase-free water previously heated at 90°C. The RNA samples were labelled and stored at -80°C until processed downstream  Libraries were constructed by NovoGene