GSM8564730: PC18; Mus musculus; RNA-Seq
Samples sorted FACS by 2C-GFP fluorescence and 2C-GFP positive and negative samples collected separately. RNA was extracted, purified and DNAse-I treated using the RNEasy-mini kit, according to the manufacturers protocol (Qiagen). Purified RNA was used for RNA-seq library preparation by polyA enrichment using a strand-specific method by Novogene.