GSM9195569: MN01MPs_Aorta; Mus musculus; RNA-Seq
A single-cell suspension of aortic cells was prepared using enzymatic digestion. Briefly, whole aortas from three mice were pooled, minced, and digested in 1× aortic dissociation enzyme solution (125 U/mL collagenase type XI, 60 U/mL hyaluronidase type I-S, 60 U/mL DNase I, and 450 U/mL collagenase type I) for 1 hour at 37 °C. The suspension was then passed through a 30-μm filter. Single-cell RNA-seq libraries were generated using the 10x Genomics Chromium Single Cell kit according to the manufacturer's protocol.