GSM7383210: SKOV3 cells, sgNC_3; Homo sapiens; RNA-Seq
Total amounts and integrity of RNA were assessed using Qubit fluorometer and the Agilent 2100 system (Agilent Technologies, CA, USA). Sequencing libraries were constructed by NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA). Qubit2.0 Fluorometer, Agilent 2100 bioanalyzer and Quantitative real-time PCR (Q-PCR) were carried out successively to ensure the quality of the library.