GSM9553688: LAbrini_syngas_B3; Clostridium autoethanogenum; RNA-Seq
Frozen pellets were thawed and total RNA was extracted using cellular lysis in RNase-free acid-washed glass beads (Merck) and RNeasy mini kit (Qiagen) with off-column DNase treatment followed by enrichment and purification using RNA Clean & ConcentratorTM (Zymo Research). Ribosomal RNA was removed with QIAseq® FastSelect™ Kit (335925; Qiagen). Stranded mRNA libraries were prepared with QIAseq® Stranded RNA Lib Kit (180743; Qiagen). Constructed mRNA libraries were prepared for sequencing using the NextSeq 2000 P1 300 cycles sequencing kit (Illumina).