DNA-Seq of anaerobic sludge
Paired-end library was constructed using NEXTFLEX Rapid DNA-Seq (Bioo Scientific, USA). Adapters containing the full complement of sequencing primer hybridization sites were ligated to the blunt-end of fragments. Pair-end sequencing was performed on the Illumina NovaSeq (Illumina Inc., USA) platform by Majorbio (China) with NovaSeq 6000 S4 Reagent Kit v1.5 (300 cycles) according to the manufacturers instructions (www.illumina.com).