GSM8671146: Endothelial cells (DAPI-, CD31+, CD45-) from B16-OVA-VEGFC tumors from Ifnγr1iProx1 (Cre plus) mice; Mus musculus; RNA-Seq
Tumors were harvested and digested with 220 U ml–1 collagenase IV (Invitrogen, 17104019) and 80 U ml–1 DNASE I (Sigma-Aldrich, 04536282001) for 30 min at 37°C. Digests were then passed through a metallic mesh and a 70-μm pore filter. Single cell suspensions were stained for Live/Dead with DAPI, CD45 (FITC), CD31(PE) Libraries were prepared using single cell 3′ reagent kits v3.1 (Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v3.1, 16 rxns PN-1000121; 10x Genomics)