RNA transcriptome data from C57BL/6 tissue resident peritoneal macrophages over expressing microRNA 708 or control
RNA transcriptome data from C57BL/6 tissue resident peritoneal macrophages over expressing microRNA 708 or control
Resident peritoneal macrophages (pMΦs) were obtained via peritoneal lavage with 5 ml lavage solution (PBS (Invitrogen) supplemented with 5 mM EDTA and 4% foetal calf serum (FCS)). Lavages of the same genotype were pooled together for flow cytometric cell sorting prior to nucleic acid extraction.  Total RNA extraction was performed using miRNeasy Micro Kit (Qiagen) accordingly to manufacturer's instructions. Paired-end reads from Illumina sequencing were trimmed with Trim Galore and assessed for quality using FastQC using default parameters. Reads were mapped to the mouse GRCm38 reference genome using STAR and counts were assigned to transcripts using featureCounts with the GRCm38.84 Ensembl gene build GTF. Both the reference genome and GTF were downloaded from the Ensembl FTP site. Differential gene expression analyses used the DESeq2 packageGenes were discarded from the analysis differential expression failed to be significant (significance: adj.pval < 0.05, Benjamini-Hochberg correction for multiple testing).