RNA-seq of Auricularia sinodelicata
In this study, mRNA with a poly(A) tail was enriched from total RNA using Oligo(dT) magnetic beads. The mRNA was fragmented into approximately 300 bp fragments by ionmediated fragmentation.A fragment length of around 300 bp was chosen because the adapter length is fixed. If the fragmented fragments are too short, the proportion of adapter sequences will be excessively high, thereby reducing the proportion of valid data.If the fragments are too long, they will hinder cluster generation during sequencing. Using the fragmented RNA as a template, the firststrand cDNA was synthesized with random hexamer primers and reverse transcriptase, followed by secondstrand cDNA synthesis using the firststrand cDNA as a template.