GSM8885926: Breast epithelial, EV, rep3; Homo sapiens; RNA-Seq
RNA was extracted using the Quick-RNA Microprep Kit (Cat# R1051, Zymo Research) according to the manufacturer's protocol. Libraries were prepared from 300 ng RNA with the KAPA RNA HyperPrep Kit with RiboErase (HMR) (Cat# KK8502, Kapa Biosystems). RNA fragmentation was performed for a desired library insert size of 200-300 bp by fragmentation for 6 min at 94 °C.  Library concentrations were determined using the Qubit HS kit (Cat# Q32854, Invitrogen). Library size distribution was determined using a High Sensititivy DNA analysis (Cat# 5067-4626, Agilent) on a Bioanalyzer 2100 (Agilent). Libraries with an average between 300-400 bp were loaded on the NextSeq 2000 system (Illumina).