Metagenomic of COI Amplicons: Sample1 Nanopore
DNA was extracted from pulverized frozen yard waste samples. Amplicons were generated with  COI PCR primers, gel purified and then barcoded with ONT barcodes from the PCR Barcode Kit I (EXP-PBC001).  Sequencing libraries were prepared with the Ligation Sequencing Kit (SQK-LSK110) and purified according to standard protocols. Nanopore MinION sequencing was performed with the Mk1C sequencer with MinION FLow Cell v. R9.4.1 according to standard protocols. After sequencing, the "high accuracy base calling" model was used, with exclusion of reads that did not pass the minimum q-score threshold of nine.