GSM8447685: GR cell, glucocorticoid receptor ChIP, vehicle treated; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 min.  After quenching and cell lysis, chromatin was sonicated using 250bp sonicated ChIPSeq samples were obtained using bead size exclusion.  NGS libraries were prepped using Swift's 2S Sonic DNA library kit (Cat. No 42024) with Swift unique dual 8bp indices.  3-5ng total DNA was used as starting material for both the input and ip samples.  The libraries were subjected to PCR amplification for 3 cycles followed size selection using Agencourt AMPure XP beads from Beckman Coulter.  The average library size ranged from 437bp to 593bp.  The libraries were validated using Agilent's HS DNA kit on a 2100 bioanlyzer prior to being submitted for sequencing.