GSM9486975: NGS_polyA_U2OS_Rep2; Homo sapiens; RNA-Seq
Total RNAs were then extracted using the FastPure® Cell/Tissue Total RNA Isolation Kit V2 (Vazyme Biotech Co., Ltd., RC112-01). For the polyA-based mRNA enrichment, mRNAs were captured using VAHTS mRNA Capture Beads (Vazyme Biotech Co., Ltd., N401). For the rRNA-depleted method, ribosomal-depleted RNA was purified with Ribo-off rRNA Depletion kit (Vazyme Biotech Co., Ltd., N406). Then, the RNA-seq libraries were constructed with VAHTS Universal V8 RNA-Seq Library Prep Kit (Vazyme Biotech Co., Ltd., NRM605).