GSM9630449: RAW,rep1; Mus musculus; RNA-Seq
Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). RNA integrity was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), and samples with RNA integrity number (RIN) > 8.0 were used for library construction. Sequencing libraries were prepared using the Illumina TruSeq RNA Sample Prep Kit (Cat# FC-122-1001, Illumina, San Diego, CA, USA) starting with 1 µg of total RNA per sample. Library preparation followed the standard Illumina protocol, including poly-A selection, fragmentation, first- and second-strand cDNA synthesis, end repair, A-tailing, adapter ligation, and PCR amplification.The resulting libraries were quantified by Qubit fluorometer and assessed for size distribution on a Bioanalyzer, then sequenced on an Illumina NovaSeq 6000 platform (or appropriate instrument) to generate 150 bp paired-end reads.