GSM9090821: hiPSC-CM, D20, Sparse; Homo sapiens; RNA-Seq
On day 20, cells from both density conditions were dissociated into single-cell suspensions and loaded into the Chromium Controller (10x Genomics) for single-cell barcoded droplet generation, according to the manufacturer's protocol (CG000204-Rev D). This step was done by following the 10x genomics single cell 3' end ibrary preparation protocol. Briefly, only the 3' end of mRNA fragements were used to make library. One end of the fragements were added P5, read1 illumina sequencing primer, 16 base pairs of 10x barcode, and UMI sequences; The other end ligated with P7, sample index sequences, and illumina read2 sequencing primer sequences.