GSM9336142: retina_ 3 day; Mus musculus; RNA-Seq
Samples were taken by the suction blister method.Subsequently tissues were enzymatically digested, using trypsin for 45 minutes at 37°C. The resulting single cell suspension was then passed through 100µm and 40µm nylon meshes and through a dead cell removal column before further processing. Single-cell RNA-seq was performed using the 10X Genomics Chromium Single Cell Controller with the Chromium Single Cell 3′ V3 Kit following the manufacturer's instructions.