GSM8534067: NIPBL-D7_release_dTAG_120min_4sU_15min_R3_SLAM; Homo sapiens; OTHER
RNA was extracted using TRIzol and purified using Direct-zol RNA Miniprep Kit (Zymo #R2052). Iodoacetamide conversion was performed using SLAMseq Kinetics Kit – Anabolic Kinetics Module (Lexogen #061), with subsequent purification using either ethanol precipitation or RNA Clean & Concentrator-5 kit (Zymo #R1013). Sequencing libraries were prepared using Zymo-Seq RiboFree Total RNA Library Kit (Zymo #R3003), according to manufacturer's instructions.