Illumina NovaSeq 6000 paired end sequencing
mRNA were tagged with UMIs using the SMART-Seq� Total RNA Pico Input with UMIs (ZapR� Mammalian). The UMIs were attached to mRNAs during the first strand-cDNA synthesis, prior to PCR amplification.   Subsequently, mRNAs were PCR amplified with 3 cycles of amplification for the initial library PCR and 12 cycles of amplification for the enrichment PCR