GSM8816220: PbMORC_ChIP_1; Plasmodium berghei ANKA; ChIP-Seq
Infected blood was filtered with Plasmodipur to remove lymphocytes. The samples were then fixed with 1% formaldehyde for 60 min at 30 °C with shaking. After erythrocyte lysis in 1.5 M NH4Cl, residual cells were lysed in SDS lysis buffer. The lysates were sonicated with Bioruptor in the following condition: 30/30 s interbals for 20 times with middle power. After centrifugation of the sonicated lysates, the supernatants were subjected to immunoprecipitation with anti-GFP antibodies conjugated on Dynabeads protein A. Libraries were constructed using KAPA Hyperprep kit (KAPA Biosystems),