GSM8676679: Triad1 shRNA polysomal RNA rep 4; Homo sapiens; RNA-Seq
Total RNA was harvested using Trizol kit (Ambion).  250 ng of total RNA was used for the construction of sequencing libraries. Polysomal RNA was harvested using AllPrep RNA/Protein kit (Qiagen). 1000 ng polysomal RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using the Illumina TruSeq Stranded mRNA-Seq Library Prep kit.