GSM9047452: S3 array, acidic patch mutant octamer chromatin, 100uM ATP; Mus musculus; OTHER
A 3.2kb fragment of Cyp3a11 was amplified from the Mus musculus genome and cloned into puc18. The 'S3' array sequence used in the paper was then excised from the plasmid via restriction enzyme digestion and this sequence was assembled into chromatin via salt gradient dialaysis. Chromatin was remodeled with ATP-dependent chromatin remodelers, then footprinted via SAMOSA-ChAAT. After footprinting, reactions were incubated with Proteinase K. DNA was purified from reactions via SMRTBell Beads. Samples were prepared for sequencing using Pacbio Template Express kits (v2.0 and 3.0) and PacBio adapters (with overhang).