Brassica napus agroinfiltrated control
For double-stranded cDNA (ds-cDNA) synthesis, 500 ng of total RNA was reverse-transcribed using oligo(dT) primers and the Mint kit (Evrogen, Moscow, Russia) according to the manufacturers instructions. The resulting ds-cDNA was purified using 1.8 volumes of Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA, USA) following the suppliers protocol.