GSM9336571: VSMC, Male, HGPS, P7, H3K36me3, Rep3; Homo sapiens; OTHER
Antibody associated DNA was released by incubation in 0.1% SDS, 16 mM EDTA at 50 °C for 1 h with proteinase K digestion. DNA was purified using phenol:chloroform and libraries were amplified by PCR (12–15 cycles) with Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs) using dual-indexed barcoded primers.