Metabarcoding of root tips: fungal community
For each sample we carried out two independent DNA extractions, with NucleoSpin Soil and Plant kits, using ca. 1 ml of lyophilized root tips and pooled duplicates to optimize extraction homogenization. We used primers fITS7 and ITS4 to amplify the ITS2 rDNA region. The ITS4 primer was labeled with sample-specific Multiplex Identification DNA-tags (MIDs). The amplicon library was sequenced using an Ion 318TM Chip by an Ion Torrent Personal Genome Machine at Naturalis Biodiversity Center.