GSM8045719: Rabbit ear hypertrophic sca with drug-loaded MNs treatment, rep2, scRNA-seq [MNs-2]; Oryctolagus cuniculus; RNA-Seq
The ventral epidermis and dermis of the rabbit ear scar tissues were saved and cut into small pieces to further disassociated into single cell suspension. Cell viability were detected by trypan blue staining. Single-cell gel beads-in-emulsion generation, barcoding, sample cleanup, cDNA amplification, and cDNA library construction were performed. Subsequently, cDNA library was sequenced and bioinformatic information was analyzed. Library was performed according to the manufacturer's instructions (single cel 3' v2 protocol, 10x Genomics)