GSM9045781: Lenvatinib-resistant TPC-1 cells with sh-NC, replicate 1; Homo sapiens; RNA-Seq
Total RNA was extracted from cultured cells using TRIzol reagent (Thermo Fisher) following the manufacturer's protocol. Following RNA extraction, sequencing libraries were constructed by OE Biotech (Shanghai, China) using standard protocols for the Illumina platform. This involved mRNA purification, fragmentation, cDNA synthesis, adapter ligation, and PCR amplification.