GSM9225999: IL1B 2; Homo sapiens; RNA-Seq
RNA was isolated using the Qiagen RNeasey Mini kit (Qiagen #74104) or Zymo Quick-RNA 96 kits (ZymoResearch #R-1052) with the optional on-column DNA digestion step. Isolates from cell cultures were prepared by direct lysis in RNA lysis buffer, followed by RNA isolation with the Zymo RNA extraction kits including on-column DNase treatment. Isolated RNA was stored at –80C until further processing or shipped to service providers. Libraries were prepared according to manufacturer's instructions, using NEBNext Ultra II RNA Library Prep Kit for Illumnia. Resulting libraries were sequenced on an Illumina NovaSeq6000.