Pacbio WGS of Sainfoin cultivar AAC Mountainview
High molecular weight genomic DNA was extracted from sainfoin (Onobrychis viciifolia, cultivar AAC Mountainview, clone #4). Genomic DNA was randomly sheared, followed by end repair, A-tailing, and SMRTbell adapter ligation. Failed ligation products were removed with exonucleases, and long fragments were enriched by size selection using AMPure PB beads and gel cassette. The final SMRTbell library was sequenced on the PacBio Revio platform