GSM8963129: E5-DEC-R3_S37; Homo sapiens; RNA-Seq
Cell lysates were collected by aliquoting and pelleting post-thawing.  This pellet was was flushed out by addition of 15 µl of Enhanced 2× lysis buffer (BioSpyder Technologies, Carlsbad, CA) and incubated for 10 minutes at room temperature to allow for complete cell lysis. The lysates were collected from each insert and stored at −80 °C until further processing.   Cell lysates were utilized for the Templated Oligonucleotide Sequencing Assay (TempO-Seq™, BioSpyder Technologies, Carlsbad, CA) as the method for mRNA quantitation (House et al., 2017). TempO-seq libraries were prepared using the S1500+ targeted transcriptome panel (Mav et al., 2018) consisting of 2,976 transcripts according to the manufacturer's instructions.