GSM9556209: SU-DHL-4 CFLAR knockout untreated_biol rep 3; Homo sapiens; RNA-Seq
RNA was extracted using the QuickRNA Kit (ZymoResearch) and 500 ng RNA was used for sequencing Libraries were prepared using the Illumina® Stranded mRNA® sample preparation Kit. Library preparation started with 500ng total RNA. After poly-A selection (poly-T oligo-attached magnetic beads), mRNA was purified and fragmented using divalent cations under elevated temperature