GSM9014141: hfPSCs_H9; Homo sapiens; RNA-Seq
Bulk-RNA seq: Total RNA was isolated cells using TRIzol® Reagent (MRC, Cat# RN 190). Genomic DNA (gDNA) contamination was eliminated from the total RNA by the digestion of RQ1 DNase (Promega, Cat# M610A) for 1 h at 37 °C. RNA integrity was verified by Bioanalyzer 2100 (Agilent Technologies) with RNA Integrity Number (RIN) > 8.0. Total RNA samples were tested and then enriched for eukaryotic mRNA by Oligo(dT) magnetic beads; Add Fragmentation Buffer for mRNA fragmentation; One strand of cDNA was synthesised using a six-base random primer using mRNA as a template, followed by the synthesis of the second strand of cDNA using buffer, dNTPs, DNA polymerase I and RNase H, and cDNA purification; Purified double-stranded cDNA was subjected to end repair, base A was added, sequencing junctions were added, and fragments were screened to recover cDNA around 350 bp; PCR enrichment was performed to obtain cDNA libraries.RNA-seq libraries were sequenced on Illumina NovaSeq X paktform.