GSM8381565: hNSC MEIS1/2 ChIP rep1; Homo sapiens; ChIP-Seq
Cells were dissociated with Accutase and porcessed with the truChIP Chromatin Shearing Kit (Covaris). Chromatin of 30 mio cells was sonicated in a E220 Focused Ultrasonicator (Covaris). 1.5 µg of anti-MEISa (K830) and 1.5 µg of anti-MEIS1 (K844) were used for immunoprecipitation via Dynabeads Proein-A beads over night at 4°C. Eluted and de-crosslinked DNA was was purified by MinElute columns (Qiagen). Illumina sequencing libraries were generated using the NEBNext Ultra II Library Prep Kit for Illumina (E7645) with NEBNext Multiplex Oligos for Illumina (Dual Index Set1, E7600). Size selection after PCR was performed by Pippin Prep (Sage Science), using a 2% cassette with size window set to 300-750 bp. Samples were sequenced 36bp paired-end on an Illumina NextSeq550.