RNA-seq analysis of multiple tissues in wild mung bean (Vigna radiata var. sublobata)
RNA-seq analysis of multiple tissues in wild mung bean (Vigna radiata var. sublobata)
Samples of different tissues were collected from mature Vigna radiata var. sublobata plants grown in experimental field. Samples were immediately frozen in liquid nitrogen and stored at -80Â°C until RNA extraction. Vigna radiata var. sublobata plants were grown in experimental field under standard agricultural conditions. Plants were watered regularly and fertilized according to standard cultivation practices. Total RNA was extracted using TRIzol reagent according to manufacturer's instructions. RNA quality was checked by agarose gel electrophoresis and quantified using NanoDrop spectrophotometer. RNA-seq libraries were prepared using NEBNext Ultra RNA Library Prep Kit. mRNA was enriched by poly-A selection. Libraries were quantified by Qubit and size distribution was checked by Bioanalyzer.