Omni-C sequencing of Sepia officinalis
The library was prepared from brain tissue using the DovetailTM Omni-C Kit (Dovetail Genomics, Cat. No. 21005) according to the manufacturers protocol. Briefly, chromatin was fixed in place in the nucleus. Fixed chromatin was digested with DNase I then extracted. Chromatin ends were repaired and ligated to a biotinylated bridge adapter followed by proximity ligation of adapter-containing ends. After proximity ligation, crosslinks were reversed and the DNA was purified from proteins. Purified DNA was treated to remove biotin that was not internal to ligated fragments. The sequencing library was generated using Illumina-compatible adapters. Biotin-containing fragments were isolated using streptavidin beads before PCR enrichment of the library. The library was sequenced on an Illumina NextSeq 2000 platform to generate 400 million 2 x 150 bp read pairs.