RNA_Head_1 of sequencing of D. ruficollis
This study aims to characterize the transcriptomic profile of head tissue through RNA sequencing. High-molecular-weight (HMW) mRNA was extracted from head tissue and enriched using polyA selection to construct strand-specific cDNA libraries. Sequencing was performed on the DNBSEQ-T7 platform with 150bp paired-end reads. Three biological replicates were included to assess technical reproducibility. The data will be used for gene expression profiling, differential expression analysis, and transcriptome annotation.