Genome resequencing of Vigna radiata: a no-pollen male sterile mutant vrnpms, plant #2 (CRX1657638; CRA025244)
The amount of 1-2 ug genomic DNA was randomly fragmented by Covaris system, The fragmented DNA was selected by AgencourtAMPure XP-Medium kit to an average size of 200-400bp. The selected fragments were through end-repair, 3'adenylated, adapters-ligation, PCR Amplifying and the products were recovered by the AxyPrep Mag PCR clean up Kt. The double stranded PCR products were heat denatured and circularized by the splint oligo sequence. The single strand circle DNA (ssCir DNA) were formatted as the final library and qualified by QC.