venom-gland transcriptome raw sequence reads (SRA) of Wagler's Pit Viper
For mRNA enrichment and purification, oligo dT selection was done using polyadenylated mRNA (poly(A)+ mRNA) purified with oligo (dT) magnetic beads from 20 ug/mg of total RNA (Kingston, 2001). The quantity and quality of the purified total RNA library was assessed using Agilent 2100 Bioanalyzer (Agilent RNA 6000 NanoKit Technologies, Waldbronn, Germany). Enriched poly(A)+ mRNA isolated from the total venom gland RNA was adopted for cDNA construction. For robust and accurate transcript identification, the isolated mRNA was fragmented in standardized buffers containing divalent cations (Zn2+) (Wery et al., 2013). The fragments were then used as templates for cDNA synthesis.