RNA-Seq of homo sapiens: HeLa 48 h ANRIL KD
control (esiRLuc) and ANRIL knockdown (esiANRIL) treated HeLa Kyoto cells (5.5*105), were harvested at 24 (n = 4) and 48 h post-transfection (n=3). RNA was extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturers instructions. The integrity of extracted RNA was checked on a 2100 Bioanalyzer using the RNA 6000 Nano Kit (Agilent). NGS libraries were prepared with Illuminas TruSeq stranded mRNA LP Sample Prep Kit following Illuminas standard protocol (Part # 15031047 Rev. E) but using  of the reagents according to the in-house protocol of the Genomics CF (IMB Mainz). 250 ng total RNA was used as starting material and libraries were amplified in 10 PCR cycles.