GSM8962363: Total tumor, Vehicle, 3; Mus musculus; RNA-Seq
Tumors were harvested, dissected, and placed in Liberase TL (0.2 mg/ml). Tumors solutions were homogenized using a gentleMACS Dissociator, treated with 0.002% EDTA and 10% FBS, and passed through a 70-micrometer filter to remove clumps. Cells were then frozen in media with 10% DMSO until encapsulation. Chromium NextGEM Single Cell 3' v3.1 (10x Genomics)