GSM9337619: CD4+ cells, Cytosporone B, 2 Hours; Mus musculus; RNA-Seq
After 2 h of culture , cells were lysed in RLT buffer supplemented with 1% β-mercaptoethanol. Total RNA was extracted using the RNeasy Plus Kit (QIAGEN) according to the manufacturer's instructions. Messenger RNA libraries for RNA-seq were prepared from high-quality total RNA using the Illumina TruSeq Stranded mRNA Library Preparation Kit, following the manufacturer's protocol. Libraries were sequenced on an Illumina NovaSeq X Plus platform (paired-end, 2×101 bp) by Macrogen Japan Co., Ltd.