RNA-seq of zea mays
RNA was extracted separately from leaf and root tissues of uniformly grown plants under HN and LN conditions, with two replicates, on the eighth day of hydroponic growth. RNA-seq libraries were constructed using the VAHTS Universal V8 RNA-seq Library Prep kit, pooled, and sequenced on a DNBSEQ-T7, with a read length of 2150 nucleotides and an average depth of 20 million paired-end reads per sample. The RNA-seq reads were processed as described, and transcripts per million (TPM) for each gene were calculated.