m6A-RIP sequencing from primary human foreskin keratinocytes (HFKs) and HPV 31 positive cervical keratinocytes (CIN 612).
m6A-RIP sequencing from primary human foreskin keratinocytes (HFKs) and HPV 31 positive cervical keratinocytes (CIN 612).
Primary cells (CIN612 and HFKs) were treated with versene to remove NIH-3T3-J2 feeder cells. All samples were then harvested and collected for RNA extraction. Whole cell RNA was extracted using a Qiagen RNeasy mini kit and RIN values were verified to be >6 CD Genomics performed both the library preparation and sequencing. RNA was converted to cDNA before generating libraries using random primers