Genome seq of edited H99 callus in maize
To identify and quantify any off-target mutations created during the NRT1.1 CRISPR/Cas9 experiment, genomic DNA was extracted from NRT1.1-E1 callus tissue for deep sequencing. Genomic library preparation was conducted using the Nextera DNA Flex Library Prep Kit (Illumina Cat #: 20018704) according to the manufacturers protocol and sequencing was performed on an Illumina NovaSeq6000.