RNA-Seq of Xanthomonas oryzae pv. oryzae strain N1
Total RNA was extracted from tissue samples and its quality assessed. rRNA was removed from total RNA to enrich mRNA for prokaryotes. mRNA was fragmented to approximately 200 bp, reverse-transcribed into cDNA, and second-strand cDNA containing uracil was synthesized. After end repair and A-tailing, adapters were ligated, and the second strand was digested using UNG enzyme. Libraries were PCR-amplified, quantified, pooled, and sequenced on a next-generation platform with paired-end reads (2150 bp/300 bp). This workflow ensures high-quality transcriptome analysis suitable for various biological samples.