LRS5 (CRX1773455; CRA026688)
Fragmented mRNA was used as a template with random oligonucleotides as primers to synthesize the first cDNA strand via M-MuLV reverse transcriptase. RNaseH degraded the RNA strand, and DNA polymerase I synthesized the second cDNA strand from dNTPs. The purified double-stranded cDNA underwent terminal repair, A-tailing, and adapter ligation. cDNA of about 250-300bp was selected with AMPure XP beads, amplified by PCR, and purified again with AMPure XP beads to create a library. submitted_instrument_model: Illumina NovaSeq 5000