GSM9016259: Human ALS, rep 75; Homo sapiens; miRNA-Seq
RNA extraction RNA was extracted from plasma of ALS patients by adding 5 volumes of Qiazol to one volume of plasma, followed by miRNeasy micro kit (Qiagen) according to manufacturer's protocols and RNA yield was quantified using Qubit fluorometer Qubit fluorometer (RNA broad range assay kit; ThermoFisher Scientific). small RNA libraries were constructed using the QIAseq miRNA library kit following manufacturer's instructions.