GSM9096762: ATCC19606 lpxC knockout mutant biol rep 3; Acinetobacter baumannii; RNA-Seq
Total bacterial RNA extraction was performed using RNAprep PureCell/Bacteria Kit (TIANGEN) following manufacturer's protocols. Ribosomal RNA was removed from the total RNA and then precipitated with ethanol.   After fragmentation, the first strand cDNA was synthesized using random hexamer primers.   During the second strand cDNA synthesis, dUTPs were replaced with dTTPs in the reaction buffer.   The directional library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection