GSM9369196: In vitro Cas13 cleavage of random substrate library (PspCas13b; no protein; replicate 1); synthetic construct; OTHER
Cleavage products were collected and purified with acid-phenol:choloform and ethanol precipitation Random 35-nucleotide-containing RNA substrates were incubated with and without Cas13 proteins. Input and cleaved RNAs were collected with acid-phenol:chloroform extraction and ethanol precipitation. 3´ adaptor ligation was then performed. The RNA was subsequently subjected to reverse transcription. A total of 3 rounds of PCR were performed to amplify the libraries.