GSM8534852: VCaP, SQK-RNA002, untrimmed; Homo sapiens; RNA-Seq
Total RNA was extracted  using the RNeasy Mini Kit (Qiagen, Cat# 74104) according to the protocol of the manufacturer. The quality and concentration of RNA were assessed using an Agilent 2100 Bioanalyzer. Poly(A)+ RNA was then enriched from the total RNA using the Dynabeadstm mRNA Purification Kit (Invitrogen, Cat# 65001), which utilizes oligo (dT) beads for selective mRNA binding.