GSM8534682: P1_F, vehicle, replicate 2; Mus musculus; ATAC-seq
Animals were anaesthetized on ice, 6 hours post-injection, followed by dissection of the entire neocortex on ice, excluding the amygdalar regions. Followed by a nuclei isolation protocol, GFP+ and 7-AAD+ nuclei were collected into cold PBS with 0.1% BSA by FACS. The snATAC-seq libraries were constructed using the Chromium Next GEM Single Cell ATAC 40 Reagent Kits v2 (10x Genomics) following the manufacturer's protocol.