GSM9555944: N2, sRNA, rep1; Caenorhabditis elegans; ncRNA-Seq
RNA-Seq and sRNA-Seq samples were extracted with Trizol, in the case of sRNA-seq samples with treated with RppH. ChIP-Seq samples were crosslinked by 1% formalaldehyde and sonicated using a Diagenode Bioruptor Pico. ChIP libraries were prepared using a standard paired ended DNA 150bp library. RNA libraries were prepared using a standard protocol with polyA enrichment. sRNA libraries were enriched for 22G-RNAs with RppH and sequenced using a standard sRNA library preparation protocol.