GSM8885076: RIP IP rep2; Mus musculus; RIP-Seq
BMDCs were dissolved in RIP 1ysis buffer and incubated with magnetic bead-YTHDF1 antibody RIP immunoprecipitation buffer. Then the obtained immunoprecipitation was washed with RIP buffer and purified with proteinase K to get the immunoprecipitation RNAs. RIP lysis product and place without immunoprecipitation were applied as “input”. Co-immunoprecipitated and input RNAs were extracted with Trizol (Invitrogen). For RIP-sequencing, rRNAs were depleted based on the NEBNext rRNA depletion kit, then was used for library construction with the NEBNext® Ultra™ RNA Library Prep Kit for Illumina®. Library quality was assessed on the Agilent Bioanalyzer 2100 system. The libraries were sequenced on an Illumina Novaseq platform with a paired-end read length of 150 bp.