Oxford nanopore sequencing of a normal tissue adjacent to a ccRCC tumor
Ultra-high molecular weight (UHMW) DNA was extracted using SP Tissue and Tumor DNA Isolation kit (Bionano Genomics), according to the manufacturers protocol. Samples were prepared for sequencing using Ligation Sequencing Kit V14 (SQK-LSK114, Oxford Nanopore Technologies, UK) according to protocol with a starting DNA amount of 1 g. Whole genome sequencing was performed on a P2-Solo device using R10.4.1 Flow cells (FLO-PRO11, Oxford Nanopore Technologies).