Target enrichment of Sarascelis_chaperi_HW_0204 using UCE Arachnida 1.1Kv1 probes.
Libraries were prepared and enriched following the protocol in Wood et al (2018), with the following modifications. We enriched the pools using the Arachnid-specific probes-set (Arachnida 1.1Kv1) (Faircloth 2017, Starrett et al. 2017).  The UCE enrichments were performed following version 5.02 of the myBaits protocol (Diacel Arbor Biosciences). Hybridization reactions were incubated for 24 h at 60 C. Following enrichment, the cleaned pools were quantified using Broad Range Qubit fluorometry (Life Technologies, Inc.) and were combined into a single pool of equimolar concentration, and size selected for 250-1000 bp using a 0.5X-0.9X two-tailed KAPA bead clean-up. The pooled libraries were sequenced on a paired-end run on an Illumina HiSeq (2x150bp rapid run).