GSM8708184: IP_KObre2_2; Aspergillus flavus; ChIP-Seq
For ChiP-seq, the mycelium were cross-linked with 1% formaldehyde for 10 min at room temperature, after which 0.125 mol/L glycine was added, and the mixture was rested for 5 min to terminate the cross-linking reaction. Nuclei were collected by centrifuging the samples at 2,000 g for 5 min at 4 °C after being treated with lysis buffer (ECOTOP, ES-8523-500). To fragment chromatin DNA, nuclei were treated with nucleus lysis buffer and sonicated. For the lysis-sonicated chromatin, 20 μL of chromatin was stored at -20 ° C and used as input DNA， 100 μL chromatin Immunoprecipitation with H3K4me3 antibody (CST, 9751) ，10 μg H3K4me3 antibody (CST, 9751) was used to immunize 100 μL of chromatin Precipitation reaction, 4 ° C overnight.After adding 30 μL protein magnetic beads, the samples were further incubated for 3 hours to form magnetic beads/antibody/protein/DNA complex. For ChiP-seq, high-throughput DNA sequencing libraries were prepared using a I NEXTFLEX® ChIP-Seq Library Prep Kit (NOVA-514120, Bioo Scientific). The library products corresponding to 200-500 bp were enriched, quantified, and sequenced on Illumina® Sequencing（NOVA-514120，Bioo Scientific） and sequenced on Illumina Xten with PE 150 method.