SfurSNMP2a-1 (CRX1566786; CRA023920)
The mRNA was purified from the total RNA using polyT oligo attached  magnetic beads. Sequencing libraries were generated from the purified mRNA using  the V AHTS Universal V6 RNA-seq Library Kit for MGI (Vazyme, Nanjing, China)  following the manufacturer's recommendations with unique index codes. The Library  quantification and size was assessed using Qubit 3.0 Fluorometer (Life  Technologies, Carlsbad, CA, USA) and Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Subsequently, sequencing was performed on a DNBSEQ-T7 platform by Frasergen Bioinformatics Co., Ltd. (Wuhan, China).