RRBS of genomic DNA from a CLL patient
RRBS libraries were prepared using the Tecan Ovation RRBS Methyl-Seq System with ~40 ng input DNA per sample (range 26–53 ng) and half-scale reactions. Libraries were PCR-amplified (12 cycles) and sequenced as paired-end 150 bp reads on an Illumina NovaSeq 6000 (SP flow cell) with a Tecan custom read 1 primer, yielding ~12.5 million read pairs per sample on average.