GSM9045933: 10x Visium Spatial Transcriptomics, FFPE slides, patient1; Homo sapiens; OTHER
tissue optimization experiments were conducted using the Visium spatial tissue optimization slide & reagents kit (10× Genomics) to determine the RNA quality (RIN > 7.0) and optimal permeabilization time. 6-minute tissue permeabilization yielded the highest fluorescence signal in both frozen sections. The frozen tissues were placed onto Visium spatial gene expression slide (10× Genomics), fixed, and permeabilized to release RNA, which bonded to adjacent capture probes. RNA reverse transcription, cDNA synthesis, denaturation and amplification, quality control as well as spatial gene library construction were performed using Visium spatial gene expression reagents kit (10× Genomics).