GSM9226291: K17GW Left Upper Part Of The Pons; Homo sapiens; RNA-Seq
Total RNA was extracted from tissues using TRIzol (Invitrogen) according to the manual instruction.Tissue was ground under liquid nitrogen, mixed with TRIzol, centrifuged, and treated with chloroform/isoamyl alcohol. RNA was precipitated with isopropanol, washed with ethanol, and dissolved in DEPC-treated water. RNA quality was assessed using Fragment Analyzer, Agilent 2100 Bioanalyzer, or Qseq-400. Library preparation was performed using the Optimal Dual-mode mRNA Library Prep Kit (BGI) following manufacturer's protocols.