RNA-Seq of Homo sapiens: adult healthy T cells
RNA-seq libraries were prepared from 700 ng of total RNA, using the Illumina TruSeq Stranded Total RNA Sample Preparation Kit (Illumina, San Diego, California, US), according to the manufacturers protocol. cDNA libraries were checked on the Bioanalyzer 2100 and quantified by Qubit (Thermo Fisher). RNA Sequencing was performed using the Novaseq 6000 system (Illumina), generating almost 100 million of 150 bp paired-end reads per sample