RNA-Seq of H357 oral keratinicytes infected with F. nucleatum
H357 cells were infected with F. nucleatum 23736 (MOI 10:1) for 3 h when RNA was extracted using the RNeasy minikit (Qiagen). Strand-specific, polyA mRNA libraries were sequenced using the Illumina NovaSeq 6000, generating paired end 150 bp reads, by Novogene (Cambridge, UK). Samples were sequenced in triplicate with a minimum of 45 m reads (>6.0 Gb data) per sample with Q30 >90%. At least 94% of reads mapped to the human genome (Homo sapiens GRCh38/hg38).