RNA-seq of Bacteroides thetaiotaomicron Pbt3441::P1E6 del_bsap5i strain
In vitro RNA-seq of B. thetaiotaomicron strains with altered stain-positive subpopulation abundance. B. thetaiotaomicron strains Pbt3441::P1E6 del_bsap5i (60% stain-positive) was cultured anaerobically in TYG medium at 37 C to mid-exponential phase (OD600 0.5; 5 biological replicates per strain). Cells were harvested by centrifugation at 4 C, and pellets were flash-frozen in liquid nitrogen. Total RNA was extracted using the RNeasy Mini Kit (Qiagen), treated with TURBO DNase (Invitrogen).