GSM8962199: SK394, VD-RNA; Homo sapiens; RNA-Seq
RNA-seq: mRNA was isolated from cells using the RNeasy® Plus Mini Kit (Qiagen) according to the manufacturer's protocol. ATAC-seq: cells were treated using the Omni-ATAC protocol with some modifications. Briefly, cells were treated with lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630) and then suspended in the transposition reaction mix (50% TD Tagment DNA buffer, 5% TDE1 Tagment DNA Enzyme, 33% PBS) for 30 minutes. RNA-seq libraries were prepared using Illumina mRNA TruSeq kits as protocolled by Illumina and sequenced using an Illumina NovaSeq6000 (paired end 100 bp). ATAC-seq libraries were prepared using the Nextera Index kit (Illumina #15055289) and were sequenced on the Hi-Seq 4000 platform (50 bp single end reads).