RNA sequencing of Non-kinase Orchestrated VEGFR2–ERK Hyperactivation as a Dependency and Pharmacological Target in Tumor Angiogenesis
RNA sequencing of Non-kinase Orchestrated VEGFR2–ERK Hyperactivation as a Dependency and Pharmacological Target in Tumor Angiogenesis
Apatinib sensitive and resistant primary HUVECs were cultured in 2 μmol/L apatinib for 48 h, and collected for RNA isolation. Total RNA was extracted using the TRIzol reagent (Invitrogen, CA, USA) according to the manufacturer's protocol. RNA purity and quantification were evaluated using the NanoDrop 2000 spectrophotometer (Thermo Scientific, USA). RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) Sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina, and library quality was assessed using the Agilent 2100 Bioanalyzer System (Agilent Technologies).