GSM9518318: APEX2-DFCP1 construct, no H₂O₂ replicate 1; Homo sapiens; RNA-Seq
Molecules in proximity to APEX2 constructrs were biotinylated following hydrogen peroxide activation. Proximity-labeled RNAs were enriched using streptavidin-coated beads. RNA was extracted from streptavidin pulldown material for downstream analyses. RNA sequencing was performed on mRNAs proximity-labeled byAPEX2 constructs. RNA-seq libraries were generated from enriched RNA samples and used for transcriptome-wide analysis of proximity-associated mRNAs.