GSM9554219: 4 week old C57BL/6J (WT) mice; Mus musculus; RNA-Seq
Excised ear was finely minced, digested with collagenase type II (616 U/mL; Worthington Biochemical Corporation), dispase (2.42 U/mL; Life Technologies), and DNAse1 (80 µg/mL; Sigma-Aldrich), incubated with 100 rpm at 37°C  for 30 minutes, triturated with glass pipettes, and then filtered. CD45-CD31+PDPN+ LECs, CD45-CD31+PDPN- BECs, CD45+ cells, and CD45-CD31- cells were sorted from 8-10 mice of each of the following conditions: 4 week old WT mice, 8 week old WT mice, 8 week old Langerin-DTA mice, and imiquimod-treated mice. For each condition, the sorted cell populations were pooled together at equal proportions and, per the manufacturer's recommendations, 29,000 cells per sample were loaded into the 10x Chromium X controller to capture 20,000 cells. The 3' v4 GEM & Library construction kit(10x Genomics) was used to generate RNA libraries and sequenced with Illumina NovaSeq X platform to obtain ~30,000 reads per cell