Bulk transcriptomic profiles of mouse testes in response to male environmental exposures across age and genetic background
Bulk transcriptomic profiles of mouse testes in response to male environmental exposures across age and genetic background
Male mice were randomly assigned to one of three treatment groups: Control (CON), non-absorbable antibiotics (nABX), or low protein high sugar diet (LPHS). Young (3 weeks) and mature (12-13 months) mice in the CON group received water and standard rodent diet, nABX mice received antibiotics in their water (Neomycin, Bacitracin, Pimaricin) along with the standard diet, and LPHS mice were given a low-protein, high-sugar (LPHS) diet and water. Whole testes from FVB and C57BL/6J control and treated mice were snap-frozen in liquid nitrogen and stored at -80C. They were homogenized in Trizol with a BeadBug[TM] homogenizer, and total RNA was extracted following the manufacturer's protocol. RNA was quantified with a Nanodrop and RNA TapeStation kit. RNA-seq libraries were prepared using 100-500ng of total RNA with the NEBNext Ultra II directional RNA kit and amplified by 11 PCR cycles.