Metagenome sequencing of Sus scrofa domesticus: piglet gut microbiome
DNA extracted from piglet gut content (QIAGEN DNeasy PowerSoil Pro Kit); 300-500 bp shearing (Covaris M220); library constructed via NEBNext Ultra II DNA Kit (Illumina adapter, 12-cycle PCR); 150bp paired-end sequencing (Illumina NovaSeq 6049).