GSM8963135: KO PS low; Mus musculus; RNA-Seq
B16-F10 tumors were dissected from WT or KO mice and single-cell suspensions were obtained. PShigh and PSlow CD8+ T cells (approximately lowest and highest 1/3 staining, respectively) were enriched using FACS sorting. The freshly prepared single-cell suspension was adjusted to 700-1200 cell/μl according to the 10×Genomics Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (No. 1000268) Operation manual for computer and library construction. The constructed library was sequenced using Illumina Nova 6000 PE150 platform.