GSM9046307: Multiome, Subject 10, PBMC, vaccinated, ATAC; Homo sapiens; ATAC-seq
Single cell multiome (RNA+ATAC) sequencing (Chromium Next GEM Single Cell Multiome ATAC + Gene Expression, Document Number GC000338 Rev F, 10X Genomics) was done using PBMCs collected at day 7. Nuclei were prepared from thawed PBMCs according to 10X Genomics demonstrated protocol (Nuclei Isolation for Single Cell Multiome ATAC + Gene Expression Sequencing, Document Number CG000365 Rev C, 10x Genomics). Single Cell Multiome ATAC and Gene Expression (GEX) libraries were prepared using the Chromium Single Cell Multiome ATAC + Gene Expression platform (10X Genomics, Pleasanton, CA). 10,000 nuclei were targeted for each sample. Isolated nuclei were transposed and partitioned into Gel Beads-in-emulsion (GEMs) using the 10x Chromium Controller and Next GEM Chip J. ATAC and GEX libraries were generated from the same pool of pre-amplified transposed DNA/cDNA. Representative traces and quantitation of both libraries were determined using Bioanalyzer High Sensitivity DNA Analysis (Agilent, Santa Clara, CA). Sequencing was done on Illumina NovaSeq S4 targeting 20,000 reads per nucleus for gene expression and 25,000 reads per nucleus ATAC-seq.