GSM9630912: Mim miR-467e-5p, batch b1; Mus musculus; RNA-Seq
After transfection and fixation, cells were pelleted and total RNA was extracted from fixed cell pellets. RNA extraction and library preparation were performed from fixed material. Paired-end strand-specific total RNA-seq libraries were prepared from transfected mESCs using Illumina TruSeq Stranded mRNA Sample Preparation Kit. Libraries were sequenced on an Illumina HiSeq 4000 instrument. Note: For each library, three FASTQ files were generated (R1, R2, R3); R1 and R3 were used for alignment as the forward and reverse reads respectively; R2 (the index read) is also deposited. Read depth was approximately 40 million reads per sample.