Mycoplasma synoviae  CQTL01
The DNA fragments were first treated with g-TUBE to the appropriate size, then the fragment ends was repaired, and both ends of the DNA fragment were ligated to the connector of the hairpin structure to form a dumbbell structure called SMRTbell. The annealed smrtbell is mixed with the polymerase on the bottom of the ZWM, which will be used for the final sequencing.