GSM8381937: MCF10A cells, Cas9-expressing clone 11, gRNA library, bulk; Homo sapiens; OTHER
Genomic DNA was extracted from 2 million cells using the AllPrep DNA/RNA Mini Kit (Qiagen). gRNAs were amplified by PCR using specific primers, and library preparation was performed with the Nextera DNA Flex Library Prep Kit (Illumina) according to the manufacturer's instructions.