CJ141
Extract the total DNA from the colonic chyme, and test the concentration and purity of the DNA using a microplate spectrophotometer. Use primers 319F (5-ACTCCTACGGGA GGCAGCAG-3) and 806R (5-GGACTACHVGGG TWTCTAAT-3) to amplify the bacterial 16S r RNA gene in the V3-V4 region from the DNA sample through PCR. Detect the PCR products through 2% agarose gel electrophoresis and determine their concentration using a Qubit 2.0 fluorescence meter. Conduct sequencing and library construction using Illumina Mi Seq.