GSM8707950: h- asp1-H397A::natMX aps1∆::kanMX siw14∆::hygMX spx1∆::ura4MX biol_rep_1; Schizosaccharomyces pombe; RNA-Seq
Hot acid phenol RNA purification protocol RNA-seq libraries were prepared from polyA+ RNA using the NEB Ultra II Directional RNA library prep plus PolyA isolation module kit according to manufacturer's protocol. Indexed libraries were normalized and pooled for paired-end sequencing performed using an Illumina NovaSeq X-Plus instrument at the Weill Cornell Medicine Genome Core Facility (in New York). Stranded polyA+ RNA seq