GSM7373445: Patient_1; Homo sapiens; RNA-Seq
Fresh tumor tissue was collected from lymph nodes of four adult patients with classical Hodgkin lymphoma. . Tumor biopsies were minced, fragmented into single-cell suspensions using a 0.45-μm filter, and washed 3X with 0.04% BSA in PBS. Single-cell suspensions were loaded onto the 10X Genomics Chromium Controller. Gene expression libraries were prepared using the Chromium Single Cell 5′ Reagent Kit with v1.1 Chemistry (10X Genomics) according to the manufacturer's instructions.