Exome capture of Pinus sylvestris: mature tree needle
DNA was extracted from needle using E.Z.N.A SP Plant DNA Kit (Omega Bio-tek), fragmented using Bioruptor UCD-200 (Diagenode), and size-selected using Agencourt AMPure XP beads (Beckman Coulter). The library was prepared using Kapa HyperPrep Kit (Kapa Biosystems) with SeqCap Adapter Kit (Roche). Exome capture was performed using SeqCap EZ HyperCap (Roche) kit with SeqCap Design PiSy_UOULU bait set and SeqCap EZ Developer Reagent blocker.