Ribo-seq of mus musculus: in-vitro-differentiated WT plasma cell
Naive B cells form WT mice were cultured with Balb/c 3T3 cells stably expressing CD40L and Baff in the presence of IL-4 for 4 days and in the presence of IL-21 for 1 additional day. Live B cells were sorted, snap frozen, and stored at -80C until further processing. Ribo-seq was conducted by Gene Denovo Biotechnology.