GSM8783589: ParBF-N153A, E. coli, F, input1; Escherichia coli; ChIP-Seq
Lysates were clarified from sonicated cell and protein-DNA complexes were isolated with anti ParBF antibody A maximum of 2 ng of ChIP DNA was used to synthetize the library as described in manufacturer's instructions (Ion ChIP-Seq Library Preparation on the Ion Proton™ System - revision B - Step10). The last size selection was modified by a double size selection (0.55X/0.25X) of binding to AMPure® XP beads followed by a step of wash and elution. Then, qualification and quantification were made with the Bioanalyzer - Agilent, Santa Clara, CA