RNA-Seq of HeLa cells: ASO-NC-2
Total RNA was extracted from HeLa cells via TRIzol (Invitrogen, Cat No. NR604-01). The libraries were constructed via the VAHTS Universal V6 RNA-seq Library Prep Kit (Vazyme, Cat No. NR604-01) according to the manufacturers instructions. RNA libraries were prepared for sequencing via the BGI DNBSEQ-T7 sequencing platform, and 151 bp paired-end reads were generated. Raw reads in fastq format were first processed via fastp, and the low-quality reads were removed to obtain clean reads. The FPKM  value of each gene was calculated, and the read counts of each gene were obtained via HTSeq-count.