GSM8884015: Sh1; Mus musculus; RNA-Seq
The heart tissues were minced using scalpel blades and subsequently digested using the Multi Tissue Dissociation Kit 2 (Miltenyi Biotec, 130-110-203). Then, cells derived from mouse heart tissue were filtered through a 100 μm cell strainer to obtain a single-cell suspension. The suspension was then centrifuged at 300x g for 10 minutes to pellet the cells. To remove red blood cells, Red Blood Cell Lysis Solution (Miltenyi Biotec, 130-094-183) was utilized. Subsequently, cell debris was eliminated using the Dead Cell Removal Kit (Miltenyi Biotec, 130-090-101). Cells were processed using the Chromium Single Cell 3' Reagent v3 Kits from 10x Genomics. The workflow included reverse transcription, barcoding, complementary DNA amplification, and purification for library preparation, following the Chromium 3′ V3.1 protocol.