GSM8676489: WT mice injected with Ctr virus, rep. 2; Mus musculus; RNA-Seq
LV tissues were homogenized and RNA was extracted using Direct-zol RNA Miniprep Plus Kit (Zymo Research), according to the manufacturer's instructions Barcoded stranded mRNA-seq libraries were prepared from 700 ng of high-quality total RNA samples using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs (NEB), Ipswich, MA, USA) implemented on the liquid handling robot Beckman i7. Obtained libraries that passed the QC step were pooled in equimolar amounts.