Amplicon liberates for the V3-V4 region of the 16S rDNA
Amplicon liberates were constructed for PE250 sequencing strategy through the upgraded Illumina sequencing platform (Novogene, Germany) using bacterial fused EUB8F and 1492R primers for the V3-V4 region of the 16S rDNA. PCR products will subsequently be purified using the QIAquick PCR purification kit. A second amplification will be performed to obtain amplicons of the V4 region using 515F and 806R primers, with an Illumina adapter and an 8-nucleotide barcode at the 5-end