scRNA-seq of mouse spleen (LLHA2) (CRX1724761; CRA025780)
Single-cell suspensions were deposited onto microwell chips using the Singleron Matrix Single Cell Processing System. Subsequently, Barcoding Beads were retrieved from the microwell chip. The mRNA captured by these beads underwent reverse transcription to yield complementary DNA (cDNA), followed by PCR amplification. The amplified cDNA was then subjected to fragmentation and ligation with sequencing adapters. The construction of scRNA-seq libraries adhered to the guidelines of the GEXSCOPE Single Cell RNA Library Kits (Singleron). Individual libraries were diluted to a concentration of 4 nM, pooled, and subsequently sequenced on an Illumina NovaSeq 6000 platform, employing 150 bp paired-end reads.