RNA-seq of Bacteroides thetaiotaomicron del_bt3442-39 strain from monocolonized mouse cecal contents 48 hours post gavage
Germ-free C57BL/6J mice (n = 5 per group) were monoassociated by oral gavage with the del_bt3442-39 (10% stain-positive) B. thetaiotaomicron strain. At 48 hr post-gavage, mice were euthanized and cecal contents flash-frozen in liquid nitrogen. Samples were processed according to the PowerMicrobiome Kit (Qiagen), followed by Dnase treatment (TURBO DNase, Invitrogen) at room temperature and another round of RNA purification with the RNeasy kit and on-column DNase treatment (Qiagen).