GSM8564818: BRF22.C1,scRNAseq; Homo sapiens; RNA-Seq
Fresh tumor was minced into cubes <1mm3 and digested using LiberaseTM (Roche, Besel, Switzerland) and TrypLE™ Express Enzyme (Thermo Fisher Scientific, Walthman, MA, USA). After filtration and centrifugation, the obtained cell pellet was resuspended in PBS + 0.04% BSA at 1000 cells/µL. Cancer cell viability was microscopically assessed by Trypan blue staining. Single cell suspensions were diluted (cell number target: 10.000 per sample), loaded on a Chromium Single Cell 5' Chip (10X Genomics) and run in the Chromium Controller of the 10x Genomics Chromium X platform to generate single cell gel beads emulsions, following manufacturer's instructions. Obtained libraries were sequenced on Illumina NovaSeq 6000 platform. Chromium Next GEM Single Cell 5' Kit v2