GSM9455034: RNAseq of Marchantia Thallus after 8 days white light + 1 day in darkness + 180 minutes  Blue light - bio rep #1; Marchantia polymorpha; RNA-Seq
For each condition, three biological replicates - each containing three individual Tak-1 plants - were collected in 2 mL Eppendorf tubes containing a stainless-steel bead (5 mm) and immediately frozen in liquid nitrogen at the defined timepoints of 0 min, 30 min, 60 min, 180 min, 12 hours, and 24 hours in dark, white light, blue light, red, far-red light, respectively. All samples were stored at -70°C until the full set was collected. Tissue from the samples was disrupted using a mixer mill (RETSCH MM 400) for 4 minutes at 30 Hz, with nitrogen-based cooling breaks during the process. Total RNA was extracted using the High-Performance RNA Bead Isolation kit from the Molecular Biology Service facility at the Vienna BioCenter. The kit employs a guanidine thiocyanate-based lysis method adapted from (Boom et al., 1990), and utilizes carboxylate-modified Sera-Mag Speed beads, with processing done on the KingFisher instrument (Thermo). mRNA library preparation, including poly(A) capture and NovaSeq S4 PE150 XP RNA sequencing, was performed by the Next Generation Sequencing (NGS) VBC Core Facility. The 78 cDNA libraries, corresponding to 26 treatments with three biological replicates each.