RNA-Seq of mus musculus: EB_Pum2-KO_D12_RA
A total of 200 ng of mRNA was subjected to poly-A tail enrichment using mRNA Enrichment Magnetic Bead Kit (Vazyme, Cat# N401). After enrichment, strand-specific mRNA libraries were constructed using the RNA Library Prep Kit (Vazyme, Cat# NRM604) with dual-index primers (Vazyme, Cat# N358), according to the manufacturers instructions.