GSM9337943: E-mu-Myc-#505 cells; Mus musculus; RNA-Seq
Total RNA was extracted from E-mu-Myc-cells or from E-mu-Myc-cells-derived B-cell lymphomas, using the RNeasy mini kit (74104, Qiagen) according to manufacturer's instructions. 1 µg of total RNA was used for the construction of sequecing libraries. Libraries were generated using Truseq Stranded mRNA kit (Illumina). Libraries were then quantified with Qubit dsDNA High Sensitivity Assay Kit (Invitrogen) and pooled. 4nM of this pool were loaded on a Nextseq 500 High output Flowcell and sequenced on a NextSeq 500 platform (Illumina) with 2 × 75bp paired-end chemistry.