Genome sequence of Aureobasidium pullulans Y2311-1
PacBio libraries were prepared using the SMRTbell prep kit 3.0 (102-182-700, PacBio). Genomic DNA was sheared to 10 kb fragments with a Covaris g-Tube, size-selected, and then ligated with hairpin adapters. Sequencing was performed on the PacBio Sequel IIe platform using the Revio Polymerase Kit (102-739-100, PacBio), yielding 92,390 raw reads with an N50 of 11,756 bp and an average sequencing depth of 42 coverage. Raw PacBio subreads were processed through SMRTLink 8.0s ccs module (minFullPass = 3 and minPredictedAccuracy = 0.99) to generate high-fidelity (HiFi) circular consensus (CCS) reads. The HiFi reads were sorted by barcode, saved in fastq format, and subsequently assembled using hifiasm v0.19.5 with default parameters (6). The final assembly resulted in fourteen scaffolds (3.52, 2.57, 2.5, 2.38, 2.19, 2.09, 2.08, 2.07, 1.91, 1.49, 1.34, 1.21, 0.04 and 0.02 Mb) with an N50 value of 2,193,234 bp.