Riverside preinjection
The pool was loaded onto a MiSeq v2 Standard flow cell and sequencing was performed in a 2x250bp paired end format using a MiSeq v2 500 cycle reagent cartridge. Base calling was done by Illumina Real Time Analysis (RTA) v1.18.54 and output of RTA was demultiplexed and converted to FastQ format with Illumina Bcl2fastq v2.20.0.