GSM9451397: L. interrogans, sHPLM, 4 hours, rep1; Leptospira interrogans; RNA-Seq
Bacteria were pelleted at 3700 RCF for 30 minutes, and RNA isolated by TRIzol and chloroform extraction. 500ng to 1ug of total RNA was used for library preparation. cDNA libraries were prepared using the Qiagen FastSelect kit and Life Technologies SuperScript III RT enzyme.