RNA-Seq of wildtype Brachypodium distachyon
Sequencing Sequencing-ready libraries were produced using a QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina following standard procedures, as outlined in the respective UserGuide.Single indexed library preparation was performed to allow for multiplexed sequencing. For library preparation, 200 ng of RNA samples (12 samples) were used as an input. Prepared libraries were quality controlled on a Bioanalyzer device (Agilent), using the HS-DNA assay. Concentration of obtained libraries was quantified using a Qubit dsDNA HS assay (Thermo Fisher). A sequencing-ready pool of indexed libraries was prepared according to these quantifications.