GSM8816169: HCECs, negative control, with PM2.5 exposure for 3 hours 2; Homo sapiens; RNA-Seq
The total RNA of cells or tissues was extracted by FlaPure Animal Tissue Total RNA Extraction Kit (Genesand, RE705). Spectrophotometers (NanoDrop 2000c, Thermo Scientific) were used to detect the quality and concentration of RNA samples. NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, Beverly, MA, USA) was applied to prepare sequencing cDNA libraries.