GSM9195383: RBBP5-dTAG ES line (rWT) treated with dTAG-13, replicate 3; Mus musculus; Bisulfite-Seq
Genomic DNA extracts were procesed as per manufacturer's recommendation. Genomic DNA was digested with MspI enzyme overnight. MspI-digested gDNA was end-repaired using NEBNExt Ultra II End Prep enzyme (NEB, E7645S) and NEBNext EM-seq adapter (NEB, E7140S)  was ligated using NEBNExt Ultra II Ligation mix (NEB, E7645S).Size-selected DNA was bisulfite-converted and purified using EZ DNA Methylation Lightning Kit (Zymo, D5030). Bisulfite-converted libraries were barcoded and amplified using NEBNext Multiplex Oligos for EM-seq (NEB, E7140S) and EpiMark HotStart Taq polymerase (M0490S).Libraries were then cleaned up with Zymo Cleanup kit.