GSM9553357: Joma_n1 transfected with siRNA LKB1; Mus musculus; RNA-Seq
Total RNA was extracted using TRIzol™ (Invitrogen™), and reverse-transcribed with the iScript™ Reverse Transcription Supermix (Bio-Rad). Quantitative polymerase chain reaction (qPCR) was performed using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) and CFX CONNECT (Bio-Rad). RNA-Seq libraries were constructed with Optimal Dual-mode mRNA Library Prep Kit from Beijing Genomics Institute (BGI). A sequence read length of 150 bp was obtained by sequencing on the DNBSEQ T7 platform using the pair-end method (30M clean reads per library).