16S rRNA gene sequencing of Populus tremuloides leaf endophyte DNA: sample PBL-endo
Libraries for sequencing were prepared according to Illuminas 16S Metagenomic Sequencing Library Preparation guide, with the exception of using Qiagen HotStar Taq DNA polymerase for the first PCR (amplicon PCR). A mixture of peptide nucleotide acid (PNA) blockers oligos (PNA Bio Inc) was also included in the reaction to inhibit plant material amplification. The template specific primers were : 515F-5 (5- GTGYCAGCMGCCGCGGTAA-3) and 926R (5- CCGYCAATTYMTTTRAGTTT-3).