HCT116 cells expressing dCas9-ZIM3(KRAB)-MeCP2(t) - Day 21 - Replicate 1
gDNA samples were isolated using the Zhang Lab salt precipitation protocol. Libraries were constructed by amplifying 250ug of gDNA across 48 individual PCR reactions using Hercules II Fusion Polymerase (Agilent) and primers (with unique dual indices) designed to amplify integrated single guide RNA sequences.