GSM9014132: hfPSCs_2_ATAC; Homo sapiens; ATAC-seq
After cell passage, the cells were washed once with PBS, and 50,000 cells from each group were used for subsequent library preparation. The library construction was carried out following the manufacturer's protocol for the Vazyme Hyperactive ATAC-Seq Library Prep Kit for Illumina (TD711). In brief, cells were resuspended in freshly prepared lysis buffer and lysed accordingly. The lysate was then mixed with the fragmentation master mix and incubated at 37 °C for 30 minutes to achieve chromatin fragmentation. Following fragmentation, the reaction was terminated, and the resulting fragments were immediately purified using ATAC DNA Extract Beads. The purified DNA fragments were subsequently used for PCR amplification to generate the final libraries. All libraries were sequenced on an Illumina NovaSeq X Plus platform according to the manufacturer's instructions.