GSM8960246: DMSO Mock Rep1; Homo sapiens; RNA-Seq
Total RNA was extracted using Trizol reagent. Total RNA harvested from the experiment was combined with the reaction buffer provided in the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Bio) for reverse transcription followed by PCR amplification to give full-length cDNA. Nextera XT DNA Library Prep Kit with unique dual indexes (Illumina) was used to prepare barcoded sequencing libraries compatible with Illumina sequencing platforms. Prepared libraries were pooled and quantified using a Qubit® Fluorometer (Life Technologies).