GSM9154801: Gene expression in liver of Usp21 KO mice 1; Mus musculus; RNA-Seq
The total RNA was extracted using the ISOGEN Ⅱ reagent (NIPPON GENE, Japan). cDNA was synthesized using 0.5 μg total RNA as a template with oligo (dT) primer (Life Technologies, CA), random hexamers (Takara Bio, Japan), and M-MuLV reverse tran-scriptase (New England Biolabs, MA). RNA libraries for RNA-seq were prepared using ILLumina seq library prep kit using the manufacturers protocol