GSM8446967: 3 groups: Control, pump+NS, pump+AZD; Homo sapiens; RNA-Seq
The harvested LV tissues were minced into small pieces in a glass petri dish. Following this, the tissues were incubated in a fixation buffer containing 4% formaldehyde for 16 hours at 4°C. The fixed tissues were dissociated using the gentleMACS Dissociator (Miltenyi Biotec) as per the manufacturer's instructions. The cells were subsequently processed using the Chromium Next GEM Single Cell Fixed RNA Sample Preparation Kit from 10x Genomics. For each sample, 10,000 cells were loaded into a Chip Q for GEM generation. Reverse transcription, barcoding, complementary DNA amplification, and library preparation were executed according to the protocol outlined in CG000527_Chromium_FixedRNAProfiling_MultiplexedSamples_UserGuide_Rev_E.