GSM9554215: Nki_GFP_ctrl; Homo sapiens; OTHER
Genomic DNA was extracted using a QIAamp DNA Kit (Qiagen, 56304). A customized sgRNA library targeting 573 human E3 ubiquitin ligases (6-10 sgRNAs per gene, ~5,204 total sgRNAs) was cloned into a lentiviral backbone and packaged in HEK293FT cells.