Element AVITI paired end sequencing
0.01 - 500 µg of input DNA was fragmented using a Covaris device. Sequencing libraries were constructed using the NEBNext® Ultra II DNA Library Prep Kit for Illumina with NEBNext Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs) (New England Bioloabs). After adapter ligation, libraries were amplified for 9-15 PCR cycles and purified using Ampure XP Beads (Beckman-Coulter).