16s rRNA amplicons V3-4 region of Karenia brevis microbiome
The workflow for this 16S rRNA amplicon sequencing project begins with DNA sample extraction using the DNA PowerWater Kit. This kit is chosen for its efficiency in extracting DNA from diverse water sources. After stringent quality control validation of the DNA samples, targeted PCR amplification of the 16S rRNA gene is performed. Subsequent purification of amplicons prepares them for library construction, including barcode incorporation. Sequencing by synthesis (SBS) on the Illumina NovaSeq 6000 platform generates raw data, which undergoes rigorous quality control. Bioinformatics analysis yields taxonomic classifications and diversity assessments. The project concludes with publication-ready figures, showcasing insights derived from high-quality sequencing data.