RNA-Seq of Salix herbacea pooled tissue
The total RNA of five tissues was isolated using the RNeasy Kit (Qiagen, Hilden, Germany) and subsequently pooled based on RNA quantity for library construction. The concentration and RNA integrity were accessed using the Bioanalyzer Agilent 2100 (Agilent Technologies, Bblingen, Germany). One mRNA library was prepared with polyA enrichment and sequenced on NovaSeq PE 150 by Novogene (UK).