GSM8849225: HEK293T, RNA-seq, ND1 silencing, 48h, r3; Homo sapiens; RNA-Seq
After the treatment, the media was removed and 1mL of Trizol reagent added, and the cells lysed and resuuspended. The Trizol samples were stored at -80°C until processing. Total RNA was extracted from the aqueous phase and isolated with ZymoResearch RNA isolation kit 25. mRNA library preparation (poly A enrichment) performed at Novogene