GSM9152778: iPSC-derived spinal motor neurons, TDP-43 knockdown, batch 2, rep 1; Homo sapiens; RNA-Seq
Cells were harversted in 1mL TRIzol Reagent (Thermo Fisher Scientific) per 1-3 million cells. Samples were added with chloroform (0.2mL), mixed and centrifuged at 4°C for 15 minutes at 12,000xg after 3 minutes of resting at room temperature. The aqueous phase containing the RNA was transferred to a new tube and added with 5µg of RNase-free Glycogen (Thermo Fisher Scientific). The aqueous phase was added with 0.5mL of isopropanol, incubated for 25 minutes at room temperature and centrifuged for 10 minutes at 15,000xg at 4°C. Samples were sent to Novogene for strand-specific polyA-purified library preparation and 150bp paired-end (PE150) sequencing