RNA-Seq of Triatoma rubrofasciata
Total RNA was extracted using Trizol (Invitrogen, CA, USA). RNA purity and integrity were monitored using a NanoDrop 2000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA) and a Bioanalyzer 2100 system (Agilent Technologies, CA, USA). The mRNA was purified using Oligo (dT)-attached magnetic beads and then fragmented into small pieces. First-strand cDNA and second-strand cDNA were synthesized and purified, followed by PCR amplification of the cDNA fragments. The amplified products were purified using Ampure XP beads ( Beckman Coulter, Inc, CA, USA) to obtain the final library. After library construction, a preliminary quantification was performed using a Qubit 2.0, and accurate quantification was achieved using RT-qPCR. The insert size of the library was assessed using an Agilent 2100 BioAnalyzer. Sequencing was carried out on a NovaSeq 6000 equipment.