GSM9455120: THP-1-PTX4-OE replicate 3; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNeasy mini kit (Qiagen) according to the manufacturer's instructions. RNA quality and quantity were assessed using NanoDrop and Bioanalyzer. RNA library construction was performed by Novogene Singapore. Briefly, mRNA was enriched using oligo(dT) beads, fragmented, and reverse transcribed to cDNA. Libraries were prepared following Illumina's protocol with adaptor ligation and PCR amplification.