CUT&RUN for MG53 in BMDMs
CUT&RUN for MG53 in BMDMs
According to the kit from Vazyme (),BMDMs were digest with trypsin, collect the cell suspension, centrifuge, and discard the medium to collect cells. Binding of cells to ConA Beads Pro, and the judgment can be performed according to the actual condition.  According to the manual, this step including DNA damage repair and end preparation, adapter ligation and library amplification.