DNA sequencing of the LORE gene: P2301-35SN-mCherry-LORE plasmid
P2301-35SN-mCherry-LORE was subjected to Sanger sequencing for verification. The target fragment, LORE gene, was inserted into the vector backbone. Plasmid DNA was extracted from individual colonies and sequenced in one direction using gene-specific primers to confirm that the sequence was correct and there were no unexpected mutations. The sequencing library was derived from synthetic plasmid DNA and selectively targeted for the verification of the cloned insert.