lrRNA-Seq of small cell lung cancer cell line
Total RNA was isolated from fresh cells using TRIzol Reagent (Invitrogen, 15596026). 500ng total RNA spiked in with Sequins (Garvan Institute) and SIRV-Set 4 (Lexogen, 141.01) was used to prepare libraries with the PacBio Kinnex full-length RNA kit. Libraries were prepared and sequenced by AGRF on the PacBio Revio sequencer. Libraries were prepared according to the manufacturers specifications from total RNA.