GSM8534822: ADHD_STR_1; Mus musculus; RNA-Seq
Total RNAs were extracted from mouse PFC and STR at P24 using the miRNeasy Mini Kit (#217004, Qiagen, Hilden, Germany). Total RNA with RIN values of ≥8.9 were used for RNA-seq library preparation. mRNA was purified from 500 ng total RNA, and used for cDNA library preparation by TruSeq Stranded mRNA Library Prep (#20020594, Illumina, San Diego, CA, USA). The quality of cDNA Libraries were determined by 2100 Bioanalyzer using Agilent High Sensitivity DNA Kit (#5067-4626, Agilent Technologies). Libraries were sequenced as 101 bp PE on Illumina NovaSeq6000.