GSM8817273: IgG rep 1, Vg6+ gd T; Mus musculus; OTHER
Vg6+ Tgd17 cells were sorted from day 5 mLN cultures as CD3+ CD4- CD8- gdTCR+ ZS-Green+ Vg4-. Epicypher CUTANA CUT&RUN kit protocol was followed starting with 500,000 Vg6+ Tgd17 cells for each sample (JunB, Fosl2, or IgG control). Epicypher CUTANA CUT&RUN library prep kit (Primer Set 1) protocol was followed for preparing sequencing ready libraries.