RNA-seq or mus musclus: male BAT SVF
After passing the library check, different libraries are pooled according to the effective concentration and the target downstream data volume, and then sequenced to produce 164 bp paired-end reads. Four types of fluorescently labelled dNTP, DNA polymerase and junction primers are added to the flow cell for amplification. When extending the complementary strand of each sequencing cluster, each fluorescently labelled dNTP added releases corresponding fluorescence, which is then captured by the sequencer, and converted into sequencing peaks by the computer software, thus obtaining information about the sequence of the fragment to be sequenced. Data analysis