GSM8849356: B16-F10, Melanoma, B16-D; Mus musculus; RNA-Seq
Tumor tissues were dissected from the B16F10 mouse models. Tumors were collected 14 days post injections (n=3 for each group). Tissues were digested with agitation with 2mg/ml collagenase D and 30μg/ml DNase I for 45 minutes at 37°C. Single-cell suspensions containing tumor cells were passed through 70 μm filters and stained with LIVE/DEAD Aqua (Invitrogen; Cat #L34957) for viability, as well as APC anti-mouse CD45 (Clone 30F11; BioLegend; Cat #103112). Viable CD45+ single cells were sorted using BD FACSAriaTM III flow cytometer. Then, the cells were sent to the 10X Genomics company for library construction and sequencing.