GSM9125399: iPSC derived human organoids, 24hr, treatment; Homo sapiens; RNA-Seq
Single cells were fixed overnight at 4 °C using 4 % PFA (ProSciTech) diluted in Concentrated Fix & Permealisation buffer. After fixation, cells were pelleted at 850 x g for 5 min. Cell pellets were quenched with Quenching and Enhancer buffer (from Chromium Gene Expression Flex Library Prep kit). Glycerol was added to each sample to a final concentration of 25 % prior to storing in -80 °C.  RNA was released and captured in‑GEM using the Chromium Gene Expression Flex Library Prep Kit (10x Genomics PN‑1000414) following the Fixed RNA Profiling Demonstrated Protocol CG000478 and User Guide CG000527; this includes in‑droplet lysis of fixed cells and on‑bead reverse transcription. Chromium Gene Expression Flex Library Prep Kit  following Fixed RNA Profiling Demonstrated Protocol CG000478 and User Guide CG000527; includes in‑GEM lysis, on‑bead reverse transcription, and cDNA amplification.