GSM9554115: L-AN-5, control / rep 2; Homo sapiens; RNA-Seq
To obtain RNA a portion of cells was taken when preparing CUT&RUN aliquots, dissolved in TRI Reagent and stored at -20C until extraction. Extraction was performed according to manufacturers intructions. Purified RNA was dissolved in 20 uL nuclease-free water and split into aliquots stored at -80C. Libraries were constructed at BGI using the DNBSEQ platform