Sample2,plot1, relicate2
Genomic DNA was extracted from fungal cultures using a standard CTAB protocol. The ITS region was amplified using universal primers ITS1 and ITS4. PCR products were verified by agarose gel electrophoresis and purified using a commercial PCR purification kit. The purified amplicons were sequenced using the Sanger method on a capillary electrophoresis platform (Applied Biosystems 3730xl DNA Analyzer).