Nor3 (CRX1720220; CRA025715)
Total corneal RNA was extracted using TRIzol reagent (Invitrogen, CA, USA), and RNA purity and quantification were determined using a NanoDrop 2000 spectrophotometer (Thermo Scientific, USA). The libraries were constructed using VAHTS Universal V6 RNA-seq Library Prep Kit and sequenced using the lluminaNovaseq6000 sequencing platform. The clean reads were mapped to the GRCm39 mouse genome using HISAT2 .The fragments per kilo bases per million fragments (FPKM) of each gene was calculated and the read counts of each gene were obtained by HTSeq-count .