RNA-seq of dexamethasone-treated primary trabecular meshwork cells from Wistar rats
Bulk RNA sequencing was performed on primary trabecular meshwork cells isolated from the corneoscleral limbus of Wistar rats. Cells were cultured in vitro and treated with dexamethasone to model steroid-induced glaucoma, with untreated cells serving as controls. This design enables comparative analysis of transcriptional changes associated with glucocorticoid-induced dysfunction in trabecular meshwork cells.