16S amplicon sequencing of microbial community: PFAS sediments
DNA extracted from samples of subsurface sediments was amplified using primers specific for the V4 region of the 16S rRNA gene with a partial adapter sequence at the 5' end.  Amplified Corynebacterium glutamicum 16S rRNA was used as an internal standard.  Resulting amplified fragments were submitted to Azenta for Amplicon-EZ sequencing.