Elas02 metabarcoding; Shinnecock Bay, NY, USA
DNA was extracted with the Qiagen DNeasy Blood & Tissue Kit and                 the 12S mt rRNA marker gene from elasmobranchs was amplified with the Elas02 primers                 (orward: 5-GTTGGTHAATCTCGTGCCAGC-3; reverse: 5-CATAGTAGGGTATCTAA TCCTAGTTTG-3;                 Taberlet, 2018). PCR conditions were 95C for 5 minutes, then 35 cycles of: 95C for                 30 seconds, 65C for 40 seconds, 72C for 1 minute, and a final annealing step of 72C                 for 10 minutes.