GSM9518147: TF-1 DAC Rep 1, WGBS; Homo sapiens; Bisulfite-Seq
DNA was extracted from harvested cells using the MasterPure Complete DNA and RNA Purifiation kit. WGBS libraries were generated using NEBNext Ultra DNA library Prep kit for Illumina (New England BioLabs) according to the manufacturer's instructions with modifications. Input gDNA was spiked with 1% unmethylated Lambda DNA (Promega, cat. # D1521) to monitor bisulfite conversion efficiency. Size selection was performed using SPRIselect beads and insert sizes of 300-400bp were isolated. Samples were bisulfite converted after size selection using EZ DNA Methylation-Lightning Kit (Zymo, cat. # D5030). Libraries were sequenced on Illumina NovaSeq X Plus instrument.