HiC of Arunachali Yak
Hi-C library was prepared from blood sample using the Dovetail Omni-C Kit (Cantata Bio). PBMCs were isolated from frozen whole blood, and 1  10 cells were crosslinked with 37% formaldehyde. Chromatin was digested with Nuclease Enzyme Mix, followed by end polishing, bridge ligation, intra-aggregate ligation, and crosslink reversal per the manufacturers protocol. DNA was purified using SPRIselect beads (Beckman Coulter; Cat No: B23317), quantified with Qubit, and processed for adapter ligation and USER digestion. Streptavidin bead capture and index PCR were performed, and the final library was size-selected, purified, and quality-checked using Bioanalyzer and Qubit.