Metagenomic of COI Amplicons: Sample2 PacBio
DNA was extracted from pulverized frozen yard waste samples. Amplicons were generated with  bardoced COI PCR primers and gel purified. SMRTbell sequencing libraries were prepared and purified according to standard protocols. PacBio Sequel sequencing was performed on SMRTbell libraries bound with the Sequel sequencing polymerase according to standard protocols. After sequencing and demultiplexing, circular consensus sequencing analysis was carried out using SMRT Link 9.0 with a minimum number of passes set equal to four.