GSM8382747: CD11c+ ABCs, mice; Mus musculus; RNA-Seq
Total RNA extraction was carried out using TRIzol reagent (Invitrogen). 2ng of total RNA was used for the construction of sequencing libraries. Samples with RIN>7 were selected for cDNA amplification using the SMART-Seq HT kit (Takara, USA). cDNA samples were further tested using an Agilent 2100 Bioanalyzer (Agilent Technologies, USA). Samples with right size distribution were selected for sequencing library construction using the TruePrep RNA Library Prep Kit V2 for illumina (Vazyme, China).