HA_ChIP_R2 (CRX763846; CRA012210)
Two-week-old seedlings were ground into a fine powder and suspended in nuclear isolation buffer (10 mM HEPES [pH 8.0], 1 M sucrose, 5 mM KCl, 5 mM MgCl2, 0.6% Triton X-100, 0.4 mM phenylmethylsulfonyl fluoride, 1X protease inhibitor cocktail, 1% formaldehyde) for 20 min for crosslinking. ChIP assays were performed using a ChIP kit in accordance with the manufacturer's instructions. Then, ChIP-Seq libraries were prepared using Library Preparation Kit  in accordance with the manufacturer's instructions.