GSM9487922: hil-1(gk229), replicate 3; Caenorhabditis elegans; RNA-Seq
RNA was isolated from samples with TRIzol Reagent (Invitrogen#15596026) as per manufacturer's instructions, except 100 uL of acid-washed sand (Sigma-Aldrich #27439) was added to each sample at the beginning of the extraction protocol to aid with homogenization. RNA was eluted in nuclease-free water and stored at -80°C until further use. Libraries were prepared for sequencing using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs # E7775) starting with 1ug of total RNA per sample as input and 7 cycles of PCR. Individually barcoded libraries were pooled and sequenced on a single lane of the NovaSeq X Plus 10B flowcell to obtain 50 bp paired-end reads.