WGS of Pseudomonas aeruginosa LESB58 no-treatment evolved
genomic DNA extracted using the Qiagen DNeasy Ultraclean Microbial Kit. Next, the sequencing library was prepared using the MinION Rapid Barcoding Kit V14 (SQK-RBK114.24) following the manufacturers protocol and sequenced using MinION Mk1D (Oxford Nanopore Technologies) using a R10.4.1 flow cell. Dorado (v1.1.1, Oxford Nanopore Technologies) was used for base calling. Adapter and quality trimming was performed using dorado trim, using --sequencing-kit SQK-RBK114.24), and files exported as .fastq files.