Pacific herring genome: long read sequencing
High molecular weight DNA was extracted for PacBio sequencing using the PacBio NanoBind Pan DNA kit (Circulomics) and fragmented according to the Megaruptor 3 DNA Shearing Guide (Diagenode). Following fragmentation, the sequencing library was prepared with the PacBio SMRTbell prep kit 3.0 using the SMRTbell barcoded adapter plate 3.0, and the Revio Polymerase kit was used for binding and cleanup. Sequencing occurred on the PacBio Revio, as well as demultiplexing, quality control, and adapter trimming with Lima v2.12.0. PacBio sequencing generated 55,021,844,244 bps across 10,537,187 reads.