GSM9045935: Negative control, day 64; Homo sapiens; RNA-Seq
Single-cell suspensions from brain organoids were fixed and processed with Chromium Fixed RNA Profiling Reagent Kit (10x Genomics, Pleasanton, CA) according to the manufacturer's instructions, using the Next GEM protocol. The library was amplified in 10 indexing PCR cycles. The concentration and quality of the libraries were measured using Qubit dsDNA HS Assay Kit (Invitrogen) and Fragment Analyzer HS NGS Fragment Kit (#DNF-474, Agilent). The libraries were sequenced in paired-end dual indexing mode with NextSeq 2000 (P3 chemistry, 138 cycles). Cell-specific 10x GEM Barcodes (16 bp) and molecule-specific UMIs (12 bp) were contained within Read 1 (28 bp), and target sequence was contained within Read 2 (90 bp).