GSM9015326: NK_CD56+CD16-_Rep 2; Homo sapiens; RNA-Seq
Total RNA from 3,000–7,000 FACS-sorted HEF or reprogrammed cells was extracted using TRIzol (ThermoFischer Scientific) following the manufacturer's protocol. Total RNA from 10,000 natural cells (CD56+ CD16- and CD56dim CD16+ PBMC-NK cells) was used for comparison. cDNA was transcribed with SMARTSeq v4 Ultra-low input RNA kit (TakaraBio), amplified with 8 PCR cycles, and purified with AMPure XP beads (Beckman). cDNA was quantified using High sensitivity DNA kit on Bioanalyzer 2100 (Agilent). Library preparation was performed using the Nextera XT DNA library preparation kit (Illumina) or SMART-Seq® v4 PLUS Kit (Takara) according to manufacturer's protocol. Libraries were normalized with beads, pooled, and sequenced on Illumina NextSeq 500 platform.