GSM9154280: NOD islet CD45+ cells, 5.5 weeks, control; Mus musculus; RNA-Seq
Mice were first anesthetized with isoflurane and then euthanized by cervical dislocation. Pancreases were harvested, digested in collagenize, and islets isolated by manual handpicking under the Leica DMI1 microscope with P20 pipette into fresh D10 media. Cells were processed using the Chromium Single Cell 3ʹ Reagent v3 kit from 10X Genomics. A total of 4,000-10,000 cells per sample were used for GEM generation. Reverse transcription, barcoding, cDNA amplification and library preparation were performed per the Chromium 3ʹ v3 protocol at Genome Technology Access Center at the McDonnell Genome Institute (GTAC@MGI). Sequencing was performed on a NovaSeq6000 platform (Illumina) targeting 100,000 150,000 reads per cell or nucleus. Cells were aligned to the mouse GRCm38 transcriptome generated using the CellRanger v6 software (10X Genomics) with "include introns" options set to "False".