GSM9553594: Human LAA RNA-seq High MNP burden (H5); Homo sapiens; RNA-Seq
Total RNA was extracted from left atrial appendage tissue using TRIzol reagent (Invitrogen) according to the manufacturer's instructions. RNA integrity was assessed using an Agilent Bioanalyzer 2100. mRNA-seq libraries were prepared by Novogene using poly(A)+ mRNA enrichment with oligo(dT) beads, RNA fragmentation, cDNA synthesis, end repair/A-tailing, adapter ligation, size selection, and PCR amplification. Libraries were sequenced on an Illumina platform as paired-end reads (instrument model/read configuration as reported in the raw sequencing metadata).