GSM9154519: H1703 cells, shEIF2B5, rep4; Homo sapiens; RNA-Seq
For Bulk RNA-seq, total RNA of H358, H1703 and MiaPaCa2 cells treated with scrambled siRNAs or eIF2Bε siRNAs (four replicates each) was isolated with Trizol (Thermo Fisher Scientific). RNA-Seq libraries were prepared following the TruSeq Stranded Total RNA protocol (Illumina) according to the manufacturer's instructions.