16S metagenomic data of endophytic bacteria in F2 fungi
After the fungal DNA samples have passed the amplification test, the PCR products are purified. Then, through steps such as end repair, addition of A tails, addition of sequencing adapters, and purification, the library construction is completed. The constructed library is quantified using Qubit and Q-PCR. Once the library is qualified, it is sequenced on the NovaSeq6000 with a PE250 read length.