GSM8987128: Hydrogel_2; Oryctolagus cuniculus; RNA-Seq
Samples are homogenized in TRIzol, centrifuged with chloroform to isolate RNA, precipitated with isopropanol, washed in ethanol, and treated with DNase I. RNA is quantified (NanoDrop), assessed for integrity (Bioanalyzer, RIN >8), and stored at -80°C. Maintain RNase-free conditions and cold handling throughout. Purified RNA is fragmented, reverse-transcribed into double-stranded cDNA, followed by end repair and adapter ligation. PCR amplifies the library, which is then size-selected using magnetic beads. Quality control (Qubit quantification, Agilent analysis) ensures library integrity. Maintain nuclease-free conditions and precise fragment size control throughout.