GSM8960944: WGBS-E85-HF_B6_AAplus_female_TP155-7; Mus musculus; Bisulfite-Seq
gDNA was extracted using 1x AMPure XP beads (Beckman Coultier, A63881), after incubation with lysis buffer (10 mM Tris–HCl pH 8.3, 50 mM KCl, 2.5 mM MgCl2, 0.5% NP-40) and 0.4 mg/ml Proteinase K (Thermo Fisher Scientific, AM2546) for 1 h at 56°C and subsequently 30 min with 2 mg/ml RNAse A (Qiagen, 19101) at 37°C. IDT xGen™ Methyl-Seq DNA library prep kit (IDT, 10009824)