GSM8783907: hemocytes, WSSV uninfected; Scylla paramamosain; RNA-Seq
The isolated blood cells were washed twice with PBS After quality inspection and counting of single-cell suspension, it is generally required that the cell viability is above 80%. Gel Beads in Emulsion (GEMs) were constructed for single cell separation according to the expected number of target cells. GEMs are collected after normal formation and are reverse-transcribed in the PCR instrument to realize labeling. For GEMs oil breaking treatment, the first strand cDNA was purified and enriched with magnetic beads, then cDNA amplification and quality inspection were carried out. The qualified cDNA was constructed by the second generation sequencing library, and the quantitative quality of the library was finally tested through the experimental processes of fragmentation, connecting sequencing joints, and sample Index PCR. The constructed library was sequenced using Novaseq Xplus/DNBSEQ-T7 high-throughput sequencing platform and PE150 sequencing mode.