GSM8043031: Skimmed milk powder EV miRNA 2C; Capra hircus; miRNA-Seq
RNA extraction performed using a mirVana Total Isolation kit (life Technologies) Small RNA-Seq libraries were generated using Library prep for small RNA-seq kit (Illumina, San Diego, CA). Acrylamide gel purifications of 140-160 nt amplified cDNA (corresponding to cDNA obtained from small RNA + 120 nt from the adapters) were performed. The final cDNA libraries were checked for quality and quantified using capillary electrophoresis. Libraries were loaded in the flow cell and sequenced as single-end 50 base reads