GSM8045601: Control_rep1 ATAC-seq; Homo sapiens; ATAC-seq
ATAC-seq libraries were generated by TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme #TD502) following the manufacturer's instructions. For each sample, 10,000 fresh cells were lysed using pre-cooled lysis buffer and then centrifuged at 500 × g for 10 min at 4°C to obtain nuclei. Nuclei were resuspended in TruePrep tagment enzyme mix and incubated for 30 min at 37°C. DNA fragments were purified the with VAHTS DNA Clean Beads and then subjected to PCR amplification. After size selected by VAHTS DNA Clean Beads, the samples were sequenced by the Illumina nova6000 platform (Geekgene, China) under paired-end 150 nt mode.