GSM9553524: En1-3; Rattus norvegicus; RNA-Seq
Chondrocytes were cultured in Dulbecco's Modified Eagle's Medium (DMEM, Hyclone, UT) containing 10% fetal bovine serum (FB-1058, Biosera) and 1% penicillin-streptomycin(ST488S, Beyotime).control cells were cultured in a high-glucose DMEM medium containing 10% FBS for 24 h, while cells in the RA group were cultured in the same medium with 1 μM retinoic acid(S1653, Bimake) for 24 h. A total amount of 1.5 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.