RNA-Seq of V. anguillarum pbta mutant (sample T)
V. anguillarum RV22 pbtA null mutant was grown aerobically in CM9 minimal medium under iron-deficient condition (50 M 2,2-dipyridyl) at 15 C. V. anguillarum RV22 wild type grown in the same condition was used as reference control. Total RNA was extracted with RNAwiz (Ambion) from cuatriplicate biological samples and used to construct independent cDNA libraries. Sequencing (ca. 20 M of 2x150 bp reads) was performed in an Illumina MiSeq sequencing machine using NextSeq High Output 1x150pb kit