RNA-seq of Homo sapiens : blood serum sample
The RNA was isolated from Extracellular vesicles. Isolated and undiluted RNA was concentrated, and not further fragmented. Libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina. Due to low RNA input, libraries were subjected to 18 cycles of amplification, and NEBNext Adaptor was diluted 200 before ligation. RNA sequencing (single read, 75 cycles) was performed using the NextSeq 500/550 High Output Kit v2 and the NextSeq 500/550 instrument