16sRNA-seq of hot spring:GuiZhou,China
Take 30Ng of qualified genomic DNA and fusion primers to configure PCR reaction system, and carry out PCR amplification, use amp XP magnetic beads to purify the PCR amplification products, dissolve in the Elution Buffer. Agilent 2152 Bioanalyzer was used to detect the fragment range and concentration of the library. Detection of qualified libraries According to insert size.