GSM8041767: PP_B_brain_injury_1 [RNA_S5219Nr7]; Mus musculus; RNA-Seq
RNA was isolated from MACS-purified B cells using RNeasy Micro Kit according to the protocols provided by the supplier (Cat. 74004, Qiagen). Briefly, cells were lysed in lysis buffer on ice and centrifuged to remove cell debris. RNA was precipitated with 70% ethanol and passed through the RNA columns. After two washing steps, genomic DNA was removed using RNase-free DNase set (Cat. 9254, Qiagen). The columns containing RNA were washed with buffers and supplemented with centrifugation steps. In the end, RNA from columns was collected using RNase-free water in new Eppendorf tubes and stored at -80°C until further used for the analysis. cDNA