GSM9014648: RL-P0-1; Homo sapiens; RNA-Seq
Total RNA was extracted from ROR1+ cells using TRIzol reagent (15596-018; Thermo Fisher, USA). The analysis was performed with 3 biological replicates. The RNA was quantified via the K5500 system (Beijing Kaiao, China), and RNA integrity was assessed via the Agilent 2200 TapeStation (Agilent Technologies, USA). The sequencing libraries were generated via the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina (#7775, NEB, USA) following the manufacturer's instructions.