GSM8851325: Skin, healthy, facial, temporal region, donor 1; Homo sapiens; OTHER
Fresh frozen tissue were OCT embedded, and 10µm sections were cut with cryostat. The generation of Stereo-seq chips, CID sequence calling (short barcode sequence for identifying the location of captured molecules), tissue processing, in situ reverse transcription, amplification, library construction, sequencing, probe and section preparation, hybridization, and staining were all conducted by MIRXES, following the protocols detailed in the Stereo-seq paper "Spatiotemporal transcriptomic atlas of mouse organogenesis using DNA nanoball-patterned arrays". Single-stranded DNA (ssDNA) staining was performed, and the tissue sections were placed on the chip for fixation and permeabilization.