PacBio Sequencing of Homo sapiens: H1302 Non-Small Cell Lung Cancer Cell Line
Using PacBio's official software package SMRT Link to process the raw sequencing data, Subreads sequences are first obtained. Circular consensus sequences (CCS) are generated by correcting the Subreads. The sequences are then classified as full-length or non-full-length based on the presence of 5' and 6' primers and polyA tails. Next, full-length sequences are clustered using the hierarchical *n*log(*n*) algorithm to obtain cluster consensus sequences. Finally, the full-length sequences are polished to generate high-quality consensus sequences for downstream analysis.