ChIP-seq investigating the influence of astrocytes on the epigenetic landscape of developing cortical neurons
ChIP-seq investigating the influence of astrocytes on the epigenetic landscape of developing cortical neurons
Primary cell cultures of cortical neurons and cortical astrocytes were generated from tissue collected from embryonic day 17.5 CD1 mice and embryonic day 20.5 Sprague-Dawley rats. Chromatin immunoprecipitation was performed with the SimpleChIP® Plus Enzymatic ChIP Kit (Magnetic Beads) (Cell Signalling Technology, #9005) according to the manufacturer's protocol. Library preparation for the samples were performed using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs Inc, #E7645S) according to the manufacturer's protocol.