GSM9369246: Different PspCas13b mutant cleavage site profiles (PspCas13b_D1057A with targeting crRNA, replicate 1); Homo sapiens; OTHER
RNA was extracted by RNA mini-prep (Zymo) according to manufacturer's instruction To map the cleavage sites of various Cas13 mutants on target RNA, we carried out an in vivo adaptor ligation method. After reverse transcription, a total of 2 rounds of PCR were done to amplify the libraries with an in vivo adaptor-specific forward primer coupled with a target-gene-specific reverse primer.