GSM9489387: AngII-3; Mus musculus; RNA-Seq
RNA extracted from aortic leaflets and purified using Qiagen Rneasy kit. Bulk RNA-seq raw fast files were first subjected to quality control using FastQC  (v0.12.1) to identify potential issues such as contamination and adapter sequences (Figure S2). After trimming low-quality sequences, FastQC was rerun on the cleaned reads to ensure that all major quality issues were resolved. The sequencing reads were then aligned to the mouse reference genome (GRCm39) using HISAT2.