GSM8447503: CRC Patient Derived Organoid (PDO5) WT rep1; Homo sapiens; RNA-Seq
Tumor spheres were collected and digested with an adequate amount of trypsin to single cells. Then were sorted (DAPI (-)) to purify >95% viable cells without debris. Total RNA was extracted from cells using Qiagen RNeasy Micro Kit (#74004) following manufacturer's instructions. RNA quality was measured by Nanodrop and Agarose gel. Library was prepared according to manufacturer's instructions (PE 50bp paired-end reads)