GSM9486902: U251 cells, KD, rep3; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol reagent according to the manufacturer's instructions. RNA concentration and purity (A260/A280) were measured with a NanoDrop 2000 spectrophotometer, and RNA integrity (RIN) was assessed using an Agilent 2100 Bioanalyzer to ensure sample quality met the requirements for subsequent library construction. Transcriptome libraries were constructed using the VAHTS Universal V6 RNA‑seq Library Prep Kit strictly following the manufacturer's protocol. The procedure included RNA fragmentation, cDNA synthesis, end repair, A‑tailing, adapter ligation, and library PCR amplification. The final libraries were quality‑checked and then subjected to sequencing.