GSM7656975: ILC2,Mc5r-KO-1; Mus musculus; RNA-Seq
Total RNA was prepared, and RNA quality was assessed on an Agilent 2100 Bioanalyzer (Agilent Technologies). Total RNA was subjected to reverse transcription and amplification using the Single Cell Full Length mRNA-Amplification Kit (Vazyme, N712-02). A cDNA library was constructed with the TruePrepTM DNA Library Prep Kit V2 for Illumina® (Vazyme, TD503-01) according to the manufacturer's protocol.