GSM8447694: Human, hydrogel organoid, rep1; Homo sapiens; RNA-Seq
Cells were isolated out of the hydrogels by digestion buffer (500ug/mL collagenase in PBS) at 37C on a rotator for 30 min. Cells were washed in PBS and incubated in Trypsin at 37C until fully dissociated. Trypsin was quenched with DTI and cells were washed in PBS and passed through 40um cell strainer. 10X Genomics 3' RNA library prep was perfomed by Novogene according to manufacture's protocol