Bulk whole-genome sequencing of sterile F2 progeny (CRX1768733; CRA026575)
Genomic DNA was extracted from pooled leaf samples. Sequencing libraries were constructed through end-repair, A-tailing, and adapter ligation with Illumina TruSeq DNA HT adapters. Size selection was performed using AMPure XP beads to enrich fragments of 300 to 500 bp. The final libraries were quantified by Qubit dsDNA HS Assay and qPCR, followed by paired-end sequencing on an Illumina NovaSeq 6000 platform.