FFPE_33AN (CRX1807902; CRA027157)
To well separate the cancer regions, adjacent normal tissues and also benign controls, all FFPE tissue sections were confirmed by pathologists before macroscopic dissection. A piece of paraffin wax from each of the above cases was collected as negative control to minus any environmental contamination. Total DNA from the above samples was extracted using the QIAamp DNA FFPE Advanced kit (QIAGEN) according to the manufacturer's protocol. 1 microL of each sample was used for DNA quantification with Qubit dsDNA Quantification Assay Kits by Qubit 4 Fluorometer (Thermo Fisher).