RNA-seq of mus musculus: whole HET embryos
Total RNA was extracted using MJzol animal RNA Extraction Kit(MagBeads) following the manufacturers instructions and checked for a RIN number to inspect RNA integrity by an Agilent 4200 TapeStation .Qualified total RNA was further purified by RNAClean XP Kit and RNase-Free DNase Set. Then the libraries were constructed through mRNA isolation from total RNA, fragmentation of mRNA, the first chain cDNA and the second chain cDNA synthesis, end-repair, A-tailing, adaptor ligation and enrichment.