DNA-seq of Actinotalea fermentans
For PacBio sequencing, genomic DNA was sheared into 10-kb fragments using g-TUBEs (Covaris, USA) to prepare a long-insert library without size selection. These fragments were then ligated with universal hairpin adapters using the SMRTbell Express Template Prep Kit v 2.0 (Pacific Biosciences, USA) . Sequencing was subsequently performed using the Sequel II System Chemistry 2.