RNA-Seq of HIMECs
Total RNA were extracted using the Trizol reagent  from intestinal microvascular ECs treating with 1M Yoda1 or DMSO for 48h. The libraries for RNA-seq were prepared using the VAHTS Stranded mRNA-seq Library Prep Kit for Illumina NovaSeq 6000 platforms as recommended by the manufacturers instructions. The initial RNA-Seq reads dataset was underwent filtration and adapter trimming to secure premium-quality. High-quality reads were then mapped to the human reference genome using the Tophat v2.0.9. The expression level of each gene was quantified using HTSeq-count. Differential expression genes (DEGs) analysis was performed using the DESeq2 v1.4.5, with a stringent cut-off of |log fold change|1 and p-value <0.05. DEGs were presented in volcano plots generated using the ggplot2 package in R software.