MC38 tumor sample of control group for single cell RNAseq (CRX1712982; CRA025532)
To perform scRNA-seq on fresh tumor tissues, we collected cells by mechanical and enzymatic dissociation using enzyme cocktails. In brief, freshly isolated tissues were minced into small pieces and then treated with enzyme cocktails containing collagenase, hyaluronidase, and DNase I. The digested tissues were filtered through a 40 microm strainer to obtain a single-cell suspension. Then the single-cell suspension was then loaded onto a droplet-based platform (10X Genomics) according to the manufacturer's instructions. cDNA libraries were prepared using the Chromium Single Cell 3' v3 Reagent Kit