lrRNA-Seq of Lung Cancer cell mixture
Single cell and single nuclei cDNAs were generated from an even mixture of all 8 cell lines via FACS sorting & automated cell counting. Single nuclei were isolated using Ez Nuclei Lysis Buffer (MERCK, NUC101-1KT) and a custom protocol. 10X capture was performed using the Chromium Next GEM Single Cell 3 protocol. Reverse transcription was performed for 2 hours. PCR amplification was 11 cycles for single cells, 12 cycles for single nuclei, with an extension time of 3 minutes.