GSM8708519: Patient 12, baseline; Homo sapiens; RNA-Seq
Total RNA was extracted from frozen biopsies according to the manufacturer's protocol using the RNeasy® Lipid Tissue Mini Kit (Qiagen, #74804). Briefly, up to 100 mg of frozen tissue sections were homogenized in 1ml QIAzol Lysis reagent using the TissueLyzer II (Qiagen). The homogenate was placed on the benchtop at room temperature (15–25°C) for 5 min to allow nucleoprotein complexes dissociation. RNA was eluted with 40 µl of RNase-free water and quantified using a Qubit 4 Fluorometer (Invitrogen). Total RNA sequencing library was prepared following Illumina's TruSeq-stranded-mRNA sample preparation protocol.