GSM8817976: MDCK_stretch_replicate_1; Canis lupus familiaris; RNA-Seq
cell lysis and RNA isolation were performed using the NucleoSpin RNA mini kit for RNA purification (Macherey-Nagel, 740955.250) with DNase treatment. cDNA was synthesized using the iScript cDNA synthesis kit (Bio-Rad, 1708891). The quality and quantity of the RNA was measured using NanoDrop 2000 spectrophotometer (Thermo Scientific). Truseq RNA stranded polyA