GSM9046330: HUVECs, OS_2, IP; Homo sapiens; RIP-Seq
Total RNA was extracted using Trizol reagent (Invitrogen) and the concentration and integrity of total RNA were measured by Qubit RNA HS Assay and Agilent 2100 Bioanalyzer (Agilent Technology), respectively. MeRIP-seq libraries were  directly constructed using the KAPA RNA HyperPrep kit (KAPA Biosystems, KK8541) following the manufacturer's instructions.