GSM8447464: Patient Derived Organoid (PDO5) IKKa KO CLDN2 inh; Homo sapiens; RNA-Seq
Tumor spheres were collected and digested with an adequate amount of trypsin to single cells. Then were sorted (DAPI (-)) to purify >95% viable cells without debris (Concentration of 1000 cells/ul). Final target cell recovery of 10000 cells. Library was performed according to the manufacturer's instructions (Chromium Next GEM Single cell 3' Reagent Kits v3.1 Dual Index, 10x Genomics).