GSM9154661: BMDM TGFb 4; Mus musculus; RNA-Seq
RNA was extracted from macrophages using the RNeasy Mini kit (Qiagen) according to the manufacturer's protocol, including on-column DNase I treatment. RNA quality was validated on an Agilent Technologies 4150 Tapestation using  RNA Screentapes, and only samples with an RNA integrity number (RIN) of > 9 were used for sequencing. cDNA libraries were generated at the Functional Genomics Center Zurich (FGCZ) from RNA samples using the Illumina Stranded mRNA Prep ligation kit following the manufacturer's instructions. The quality and concentration of the libraries were determined using an Agilent Technologies 4200 Tapestation with DNA Screentapes. The libraries were pooled in equimolar amounts and sequenced on an Illumina NovaSeq X Plus sequencer (paired-end 150 bp) with a depth of at least 20 million reads per sample.