GSM8817749: HCC_ScRNAseq2; Mus musculus; RNA-Seq
Livers were isolated, tumor tissues were dissected, and prepared for single-cell isolation. Single-cell suspensions were prepared using collagenase I and DNase I digestion, followed by immune cell enrichment via density-gradient centrifugation. Chromium Next GEM Single Cell 3′ Reagent Kit v3.1 (10X Genomics) was used to generate single-cell cDNA libraries. Amplification and sequencing library preparation followed manufacturer instructions.