Illumina sequencing of SINV TE12 mCherry FMV2A grown in BHK-21 cells
Extracted RNA from BHK-21 cells with the BioRad Aurum Total RNA Mini Kit according to manufacturer's instructions. Treated RNA with Lucigen Terminator 5'-Phosphate-Dependent Exonuclease according to manufacturer's instructions. Prepared library using LIB-106b Illumina TruSeq Stranded mRNA HT Library. Sequenced using NEX-202 NextSeq 1000/2000 - P2 (100 Cycles) v3.