Shotgun Metagenomics of Marine Sediment
Shotgun metagenomic sequencing library construction was performed at the Earlham Institute (Norwich Research Park, UK), according to the LITE (Low Input, Transposase Enabled) 2.0 pipeline (Perez-Sepulveda et al., 2021). The DNA library was then sequenced on two lanes of a NovaSeq X Plus 10B flow cell, with 2x150bp paired-end chemistry.