AIV guinea fowl sequencing
Viral RNA was extracted using the RNeasy Mini Kit (Qiagen), and genome amplification was performed using the SuperScript III Platinum One-Step qRT-PCR Kit. The sequencing library was prepared using the Native Barcoding Kit 24 V14 (SQK-NBD114.24, Oxford Nanopore Technologie) and sequencing was performed on FLO114 flowcells on Minion device (Oxford Nanopore Technologies)