GSM9225866: HFD-SG34-48-replicate_1; Mus musculus; RNA-Seq
Total RNAs were extracted from embryos at specific stages utilizing TRIzol reagent.  Each RNA sample (500 ng total RNA) underwent rRNA depletion. Strand-specific libraries were constructed using the VAHTS Universal V8 RNA-seq Library Prep Kit for ILLUMINA, following the manufacturer's instructions.