GSM9554183: L-AN-5, circZNF827 kd / H3K4me3 / rep 1; Homo sapiens; OTHER
CUT&RUN was performed using the CUT&RUN Assay Kit (Cell Signaling Technologies, #86652) according to the manufacturer's protocol. Prior to footprinting, cell aliquots were thawed, counted and diluted to ensure 150,000 cells per reaction. DNA fragments were purified from enriched chromatin samples using the DNA Purification Buffers and Spin Columns (ChIP, CUT&RUN, CUT&Tag) (Cell Signaling Technologies, #14209). Resulting ssDNA samples were stored at -80C and shipped on dry ice. DNA libraries were prepared, quality tested and sequenced at BGI on the DNBSEQ platform generating paired-end 100 bp reads.