GSM9486920: CP2_5uM_USP18_O_E replicate 3; Homo sapiens; RNA-Seq
For the mRNA library preparation, we used the Illumina TruSeq stranded mRNA ligation kit. We use between 100ng to 1ug of total RNA as the input to an mRNA capture with oligo-dT coated magnetic beads. The mRNA is fragmented, and then a random-primed cDNA synthesis is performed. The resulting double-strand cDNA is used as the input to a standard Illumina library prep with end-repair, adapter ligation and PCR amplification being performed to give you a sequencing ready library Illumina TruSeq stranded mRNA ligation kit