GSM9097224: Microglia cells,MCMV Infected, untreated, 6 months; Mus musculus; RNA-Seq
Newborn Balb/c mice were infected with 200 PFU K181 <24 hours after birth. Control mice were injected with 20ul PBS control. At 2 months of age PLX group was given PLX5622 in chow for 7 days. At 6 months of age, retinal tissue was collected and microglia (CD45 int CD11b+) were sorted by FACS. Library was performed accoriding to the manufacter's instructions ( Next GEM Single Cell 3' Reagent Kits v3.1 protocol, 10x Genomics). Briefly, microglia cells were resuspended in master mix and loaded with partitioning oil and gel beads into the chip to mak GEM (gel bead-in-emulsion). The poly-A RNA from the cell lysate in the GEMs were retrotranscripted to cDNA. The barcoded cDNA was cleaned by Silane DynaBeads, aplified by PCR and the appropriate size fragmet was selected with SPRIselect for subsequent library contruction.