Transgenic Mustard
RNA sequencing of plant leaf tissue samples provided by Dr. Indraneel Sanyal at CSIR-NBRI, Lucknow. Sample IS-05 was processed to study transcriptomic responses to abiotic stress. RNA quality was verified by NanoDrop (260/280 ratios ~2.12.2, 260/230 ratios ~2.32.5) and Qubit quantification, with RIN values ranging from 5.2 to 5.8 . Libraries were constructed using [e.g., Illumina Stranded mRNA Library Prep Kit] and sequenced on [e.g., Illumina NovaSeq 6000] to generate 150 bp paired-end reads.