Chicken fecal metagenome
Fecal metagenomes from laying hens were analyzed to investigate the impact of dietary supplements (Icariin and Copper Sulfate) on gut microbiome recovery after antibiotic perturbation. A total of 45 hens were fed a combined antibiotic diet (ASP250) for 4 weeks, followed by a withdrawal period and subsequent stratification into three dietary groups: CON (basal diet), ICR (basal diet + 200 mg/kg Icariin), and CU (basal diet + 150 mg/kg CuSO4). Fecal samples were collected at five time points (Week 0, 2, 4, 6, 8) for metagenomic sequencing.