RNAseq of human: asthmatic patients before treatment with benralizumab
Total RNA was isolated from peripheral blood samples stored in RNA later at -20C using Ambion RiboPureTM -Blood kit (Thermo Fisher Scientific, Waltham, MA, USA). RNA was treated with Ambion DNAse I and concentrated with RNeasy MinElute Cleanup Kit (Qiagen, Hilden, Germany). RNA was quantified with a Nanodrop 1000 instrument, and the RNA quality was assessed by measuring the RIN (RNA Integrity Number) in a Bioanalyzer Agilent 2100 expert equipment using the Eukaryote Total RNA Nano kit (Agilent Technologies, Santa Clara, CA, USA). Globin transcripts and ribosomal RNA were removed, and RNA was cleaved to prepare RNA strand-specific libraries, generate clusters from each library, and perform a high-throughput sequencing using the Illumina HiSeq 2500 ultrasequencing platform (Illumina, San Diego, CA, USA)