ITS analysis of reed fertilizer; replicate S17
To sequence the internal transcribed spacer (ITS2) regions of the fungal 18S rRNA gene, two-step, Nextera barcoded PCR libraries were generated using ITS3 (5- GCA TCG ATG AAG AAC GCA GC -3) and ITS4 (5- TCC TCC GCT TAT TGA TAT GC -3) with 20 PCR cycles for the first step and 15 PCR cycles for the second step were created. Subsequently the amplicon libraries were sequenced on an Illumina MiSeq using a v2 500 cycles kit.