GSM9014674: Fus d14/d14, 3 months, biol rep 4; Mus musculus; RNA-Seq
Snap frozen spinal cord samples were crushed by a pestle and mortar on dry ice followed by lysis of half the sample in Qiazol (Qiagen). RNA was then extracted using the miRNeasy Mini Kit (Qiagen) as per manufacturer's instructions. RNA quality was checked by the Agilent Tapestation system and RNAs were stored at -80°C. All sequenced samples had RIN scores higher than 8. cDNA libraries were made using the KAPA RNA HyperPrep Kit with RiboErase (Roche).