GSM9486544: testicular tissue; Mus musculus; RNA-Seq
The testicular samples of MACS Tissue Storage Solution were transferred to a digestion solution containing 0.2 % collagenase IV and DNAase I. Then, they were incubated at 37 °C for 15 min. After digestion process and mechanical dissociation striking into single cells, the cell suspension was filtered through a cell strainer and converted to barcoded scRNA-seq libraries in accordance with the manufacturer's protocol (Solarbio, Beijing, China). DNBelab C series high-throughput single-cell RNA library preparation kit is based on droplet microfluidic technology and independently designed using MGIC4 droplet generation device, through efficient mRNA capture magnetic beads and droplet recognition microbeads and single-cell RNA library preparation kit The single-cell (nuclear) suspension can be quickly prepared into a dedicated library corresponding to the sequencing platform.