GSM8447633: Tumor-derived NK cells, TYK2KE, Rep2; Mus musculus; RNA-Seq
Oligo-dT primer hybridization to poly(A) tail of all RNA was performed by incubating the RNA samples with oligo-dT primer (5′–AAGCAGTGGTATCAACGCAGAGTACT30VN-3′) and dNTP mix at 72 C for 3 min. 500 pg of RNA was used as input. Reverse transcription and preamplification were performed as previously described in Picelli 2014 (Nature protocols DOI: 10.1038/nprot.2014.006). Stranded sequencing libraries were generated using Smart-seq2 protocol with Nextera DNA Library Preparation Kit (Illumina, #FC-121-1030).