GSM9097354: KIRI rep1; Mus musculus; RNA-Seq
Kidney tissues were homogenized in TRIzol, chloroform-separated, and RNA was precipitated (isopropanol), ethanol-washed, and resuspended. Alternatively, the RNeasy Mini Kit (Qiagen) was used with on-column DNase digestion to ensure genomic DNA removal. RNA libraries were prepared for sequencing using standard illumina protocols