GSM8851049: Patient 1 PBMC  High MOCA scRNAseq; Homo sapiens; RNA-Seq
PBMCs were isolated from fresh whole blood using Ficoll-Paque gradient according to manufacturer's protocol. Cells were washed twice in 1× Dulbecco's PBS and resuspended in 1× Dulbecco's PBS with 0.04% bovine serum albumin ranging from 800-1200 cells/ul. Fresh CSF was kept on ice until processing. CSF was centrifuged at 1500 rpm for 10 minutes, and supernatant was aspirated to leave 50 ul for cell counting and loading. PBMC and CSF samples were counted using 4% trypan blue and a hemocytometer. Single-cell suspensions were loaded onto the Chromium Single Cell Controller using 10x Genomics Single Cell 5' v2 kit and protocol. A target cell number of 10000 was used for PBMCs. All CSF cells were loaded. Library construction was performed by the Yale Center for Genome Analysis according to the manufacturer's (10X Genomics) guidelines. Sequencing was performed on the NovaSeq S4 instrument.