GSM8883217: Explant 3 CD45+ enriched cells for V(D)J sequencing; Homo sapiens; OTHER
Samples from the proximal, mid and distal regions of each coronary artery from explanted cardia grafts with CAV were collected. Cells from the dissociated explants were stained with aqua viability dye (Invitrogen) and anti-CD45 antibody (Biolegend). All viable cells as well as viable CD45+ cells were sorted on an Influx cell sorter. 10x Genomics Chromium Next GEM single cell 5' and V(D)J expression libraries were generated from sorted cells