GSM9047361: iTF-iPSCs, untreated, rep1 (ChIP); Homo sapiens; ChIP-Seq
Chromatin was extracted following protocols from ENCODE Consortium. Chromatin was immunoprecipitated with an anti-H3K27ac antibody (ActiveMotif). Libraries were prepared by end-repair, adapter ligation, and PCR amplification (30 sec at 98°C; [10 sec at 98°C, 30 sec at 65°C, 30 sec at 72°C] ×15 cycles; 5 min at 72°C).