GSM8447583: nasal mucosa,digested,30min,rep1; Sus scrofa; RNA-Seq
Nasal mucosae were dissected from 5-day-old Large White piglets, then were cut into small pieces, which were digested with collagenase Type IV and hyaluronidase for 30 min at 37°C. The released nasal cells were filtered through 70μm cell strainer centrifuged and re-suspended in PBS. Suspended cells activity was determined by trypan blue staining and adjusting the live cells concentration to 1000 to 2000 cells per microliter. The cells were captured with a 10× Genomics Chromium Single Cell Instrument after binding to barcoded gel beads. The raw scRNA-seq dataset were obtained after reverse transcription and RNA sequencing. These processes were completed by Gene Denovo (Guangzhou, China).