HBECs, untreated, donor 4
HBECs grown in air liquid interface culture were washed and dissociated into single cell suspensions. 15,000-20,000 viable cells were counted captured in Gel Bead-in Emulsions with the 10X Chromium 3' GEX Capture kit and the 10X Chromium controller, and subsequently used to generate a barcoded library with the Chromium Single Cell 3' Library Kit following manufacturer instructions (10X Genomics, Pleasanton, CA, USA). The library was sequenced on a NovaSeq X Plus 25B flowcell (Illumina) to generate 2x150 nucleotide paired-end reads.