GSM9045083: WT 5.(NPY+/+).504.male; Mus musculus; RNA-Seq
Whole skin was pulverized using a Biopulverizer (Biospec #59012MS), followed by a short homogenization using a bead homogenizer (MP biomedicals). Homogenized skin was phase separated using chloroform and precipitated using ethanol. Precipiated RNA was purified using the qiagen RNeasy mini kit. RNA was packaged using dry ice and submitted to Novogene for RNA-sequencing RNA with RIN values >7 were submitted to Novogene for library construction and sequencing .The following protocol was provided by Novogene "Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers."  Our library construction was performed using non-directional strands generated via second strand synthesis with dTTP. "For the non-directional library, it was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification"