Oxford Nanopore direct RNA sequencing of Leishmania donovani 1S promastigotes (20230508), raw signal
One microgram of total RNA from 1.10^8 Ld1S mid-log promastigotes was extracted using Trizol/chloroform extraction and prepared using ONT Direct RNA sequencing kit (SQK-RNA002) according to the manufacturer recommendations. Sequencing was done on a MinION (Mk1B) device with an R9.4 flow-cell (FLO-MIN106D). The submitted basecalled fast5 have been basecalled using guppy-4.2.2. For reproducibility, please use submitted fastq SRR35070793 or rebasecall using the raw signal