GSM7656313: Mild symptom, replicate1, scRNA; Mus musculus; RNA-Seq
the brain were removed and placed in a slicing chamber containing CCS,300um thick brain slabs were cut. Slabs containg the regions of cerebral cortex, striatum and thalamus were gently isolate under the sterecscope and transfered  in a 15 mL falcon tube containing 5 mLCCS+ and incubated for 30 minutes at 33℃, then transferred the tissue into  2mL of  Enzyme1 Buffer, digestion was performed at 37℃for 20minutes. The brain tissue was digest in 2mL Enzyme2 Buffer, digestion was performed on a shaker at 25℃for 20 minutes.. Tissue chunks were then carefully titrated with a series of n = 5 fire-polished, Pasteur pipets with successively smaller bores. then centrifuged at 500g for 5 minutes. The pellet was then resuspended in 3.1 mL CCS in a new tube and mixed with 0.9 mL Debris Removal Solution, slowly add 4 mL CCS on the top layer and centrifuged at 3000 g for 10 minutes, The supernatant was discarded, taking care not to disturb the cell pellet. The cell pellet was resuspended in 10 mL CCS library were performed according to the manufacter's instructions(Single Cell 3' v3 protocol, 10xGenomics).