Pacbio Hifi whole genome sequence of Aedes aegypti aegpyti: Whole single female mosquito from New Mexico, USA
We froze a single female mosquito using dry ice, then homogenized it using a sterile DNAse/RNAse free plastic pestle. We eluted this homogenate with 180microliters of PBS and processed the solution using MagAttract HMW DNA Kit, following instruction from manufacturer for frozen tissues. We sent the extracted DNA to the Maryland Institute of Genome Science of the University of Maryland for low input library preparation where it was sequenced on t he Sequel II 8M SMRT Cell.