GSM8045757: APP-PS1_6170; Sus scrofa; RNA-Seq
Hippocampal tissue from four WT pigs, four PS1 pigs and four APP/PS1 pigs were quickly removed after sacrificing the animals, dissected on ice and immediately submerged into liquid nitrogen and stored at -135oC. Tissues were homogenized in 1mL cold homogenization buffer (20mL Thioglycerol per millilitre of Homogenization solution) using a blender. Homogenates were left to settle on ice. Homogenates (200mL) were mixed with equal volume Lysis buffer and added to cartridges (Maxwell® 16 LEV simplyRNA Tissue Kit; Promega). DNAse I solution was added before samples were processed on Maxwell ® 16 Instrument. Eluted RNA was stored in -70oC. Library construction, sequencing and initial data filtering including adaptor removal were performed by BGI Europe Genome Center. Total RNA was subjected to oligo dT based mRNA enrichment. 100 bp paired-end read sequencing was performed on BGIseq-500 with DNBseq technology. More than 20 million clean reads were obtained per sample.