GSM8783372: Uninfected 2h_2; Mus musculus; RNA-Seq
RNA was collected using Rneasy Mini kit (Qiagen) Completed by Novogene. mRNA was enriched using oligo(dT) beads and rRNA was removed using Ribo-Zero kit (Illumina). mRNA was randomly fragmented using fragmentation buffer and cDNA synthesized by second strand synthesis using random primers and a custom second-strand synthesis buffer (Illumina). The cDNA was end-repaired, followed by 3' polyadenylation and sequencing adaptor ligation. The library was then size selected and enriched by PCR