GSM8783569: myotubes, siLaminsA/C, Hours72, static,rep 8; Homo sapiens; RNA-Seq
The siRNA transfections were done with Lipofectamine RNAiMAX (Life Technologies, Carlsbad, CA, 13778-150) according to manufacturer's instructions 24h and 48h after switching confluent myoblasts to differentiation medium using 50 nM of siRNA. Gene or protein of interest was analyzed 72 h after the first transfection RNA was harvested using Rneasy mini plus kit (Qiagen). 1.3 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using mRNA-Seq Library Prep Kit following manufacturer's protocols.