GSM8535071: RS4C, Treatment; Homo sapiens; RNA-Seq
Transfected  cells were lysed using RIPA buffer (Cat.# 89900, Thermofischer) and RNA was extracted using RNeasy Plus Mini Kit (Cat.# 74134, Qiagen) was used for RNA extraction, while the NEBNext® Poly(A) mRNA Magnetic Isolation Module (Cat.# E7490L, NEB)  was used to enrich mRNA from the extracted RNA.  IDT, USA, RNA libraries were then prepared using the xGenTM RNA Lib Prep 96rxn xGen RNA Library Prep Kit (Cat.# 10009814, IDT), and normalized using the xGenTM NormalaseTM Module (Cat.# 10009793, IDT).