GSM9518659: CUTLL1 cells lentivirally transduced with miR-15b/16-2 and GFP vector. Sample 1; Homo sapiens; RNA-Seq
Total RNA was isolated using TRIzol (Invitrogen) and was sent to Novogene Co., Ltd (GmbH, Germany) for library preparation and sequencing. RNA quality and quantity were assessed using the Agilent 5400 Bioanalyzer (Agilent Technologies, CA, USA) and Qubit® 2.0 Fluorometer (Life Technologies, CA, USA), respectively. 100 ng of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared by Novogene Co., Ltd (GmbH, Germany) using Novogene NGS RNA Library Prep Set (PT042)* following manufacturer´s protocols. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.