RNA-Seq of human WBC
The cells were harvested via centrifugation at 300 x g for 15 min and resuspended in phosphate-buffered saline (PBS). RNA was extracted from leukocytes using the RNeasy Mini Kit (Qiagen, Germantown, Maryland, USA). RNA quality was assessed spectrophotometrically at 230, 260, and 280 nm with a Promega GloMax Discover Microplate Reader (Promega, Madison, WI, USA). Ribodepletion was performed using the Ribo-Zero Magnetic Gold Kit (Epicenter, Madison, Wisconsin, USA). Transcriptome sequencing libraries were prepared from RNA using the TruSeq Stranded Total RNA Library Prep Kit