GSM9453605: imCAF#1-HSF1 KD; Homo sapiens; ATAC-seq
A minimum of 5 × 104 of CAFs transfected with SC or HSF1-targeting siRNAs were harvested for ATAC-seq.Cells were pelleted at 500 g for 5 min, resuspended in lysis buffer, and incubated on ice for 5 min. After addition of wash buffer, nuclei were collected by centrifugation at 500 g for 5 min. Briefly, nuclei were isolated and subjected to transposition using Tn5 transposase at 37 °C for 30 min. Following PCR amplification, libraries were purified with AMPure beads and quality was assessed using Qubit. Libraries were sequenced on an Illumina NovaSeq platform to generate 150-bp paired-end reads.