GSM9195471: HeLa cells, siControl, RNA-seq, biol rep 3; Homo sapiens; RNA-Seq
For RNA-seq, total RNA was harvested using RNAiso Plus (TaKaRa) according to the manufacturer's instructions. For ChIP-seq, cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was fragmented in a water bath using a Qsonica Q800R3 sonicator (55% amplitude, 20 s on/20 s off) for a total of 10 minutes. For RNA-seq, sequencing libraries were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA, Catalog #: E7530L) following the manufacturer's protocol. For ChIP-seq, the purified DNA was used to prepare libraries with the NEBNext Ultra II DNA Library Prep Kit (NEB, USA, Catalog #: E7645L) according to the manufacturer's instructions.