lrRNA-Seq of Lung adenocarcinoma cell line
Total RNA was isolated from fresh cells using TRIzol Reagent (Invitrogen, 15596026). 500ng total RNA spiked in with Sequins (Garvan Institute) and SIRV-Set 4 (Lexogen, 141.01) was used to prepare libraries with the PacBio Kinnex full-length RNA kit. Libraries were prepared and sequenced by AGRF on the PacBio Revio sequencer. Libraries were prepared according to the manufacturers specifications from total RNA.