GSM8534268: Wild type, Barley, rep2; Hordeum vulgare; RNA-Seq
Leaves of three-week old non-transformed wild type (WT) and HvHSFA6a overexpression transgenic plants were used to extract the total RNA with RNeasy® plant mini kit (74904; Qiagen) as per the manufacturer's instructions. During the total RNA extraction, RQ1 RNase-free DNase (M6101; Promega Corporation) was used to remove the genomic DNA. The RNA concentration was quantified by NanoDrop microvolume UV-Vis spectrophotometers (ND-ONE-W; Thermo Fisher Scientific), and total RNA (5µg) was separated on 1.2 % formaldehyde gel to determine concentration and integrity. RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.