GSM8849347: pil::Tn, 37C, replicate 3; Yersinia pseudotuberculosis; RNA-Seq
Supernatants were removed and cell pellets were resuspended in 1mL RNA Protect (Qiagen) and total RNA was isolated using the RNeasy minikit (Qiagen) according to the manufacturer's protocol. DNA was removed from samples using the TURBO DNA-free kit (Life Technologies/Thermo Fischer) and RNA quality was determined using an Agilent 2000 Bioanalyzer. Samples were DNase treated with Invitrogen DNase. Library preparation was performed using Illumina's Stranded Total RNA Prep Ligation with Ribo-Zero Plus kit and 10 bp IDT for Illumina indices.