GSM8565269: celiac ganglion of naive rat; Rattus norvegicus; RNA-Seq
The frozen samples were thawed in the ice-cold lysis buffer (Nuclei Extraction Buffer, Miltenyi Biotec), and were dissociated using the gentleMACS Dissociator. The 10× Chromium libraries were prepared according to manufacturer protocol (10× Genomics) using Chromium Single Cell 3 Reagent Kits (v3.1 Chemistry Dual Index). Briefly, libraries were quantified by Qubit 1x dsDNA HS Assay Kit (Thermo Fisher Q33231) and profiled by High Sensitivity DNA D5000 (Agilent Technologies 5067-4626).