16S rRNA V3-V4 sequencing of human gut metagenome_SAM49
Genomic DNA was extracted from human fecal samples using the QIAamp PowerFecal Pro Kit (Qiagen) with bead beating. The V3V4 region of the 16S rRNA gene was amplified using primers 341F and 806R. Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (NEB) and sequenced on an Illumina NovaSeq 6000 platform using an SP flow cell (2x250 bp paired-end).