GSM7372227: YQ0157; Homo sapiens; RNA-Seq
Total RNA was extracted from epithelial cells using the TRIzol method according to standard protocols (Life Technologies, Inc., Gaithersburg, MD, USA). The purified products were evaluated using the Agilent 2200 TapeStation and diluted to 10 pM for cluster generation in situ on the HiSeq2500single-end flow cell followed by sequencing (1 × 50 bp) on the HiSeq 2500. rRNAs were removed from total RNA using an EpicentreRibo-Zero rRNA Removal Kit (Illumina, USA). Then, the PCR products were size-selected by polyacrylamide gel electrophoresis according to the instructions of the NEBNext® Multiplex mRNA/LncRNA Library Prep Set for Illumina® and Chain specific library sequencing was performed using the UNG enzymatic method (New England Biolabs, Inc., Ipswich, MA, USA).