GSM8381487: CP1; Homo sapiens; RNA-Seq
Peripheral blood was collected into an anticoagulant tube, and peripheral blood mononuclear cells (PBMCs) were isolated using a commercial Lymphocyte Separation Medium (Beyotime, China) by centrifugation at 700g for 30 minutes. For library preparation and sequencing, each RNA sample, with an input quantity of 1 μg, underwent library construction using the Hieff NGS Ultima Dual-mode mRNA Library Prep Kit for Illumina (Yeasen Biotechnology, Shanghai, China), as per the provided protocol.