RNA-seq of BFH12 cells deleted for the CYP1B1 gene
Library was prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD (Lexogen GmbH, Austria) following manufacturer's instruction. Briefly, after first strand synthesis, the RNA was removed and second strand synthesis was initiated by random priming. The random primer also contained a partial Illumina-compatible linker sequence. The second strand synthesis was followed by a magnetic bead-based purification step. The library was then amplified, introducing the sequences required for cluster generation.