GSM8850675: emricasan treatment, 54, Week28; Mus musculus; RNA-Seq
Mice were euthanized at 28 weeks of age, and eyes were enucleated and immersed in RNAlater. Under a microscope, corneas were isolated, and endothelial cells with Descemet membrane were carefully stripped from the corneas. Total RNA was extracted using the RNeasy Micro Kit (QIAGEN) according to the manufacturer's protocol. RNA sequencing libraries were prepared using the SMART-Seq HT Kit (Takara Bio Inc.) following the manufacturer's protocol. After full-length cDNA synthesis, Illumina libraries were prepared using NexteraXT (Illumina Inc.).