GSM8565190: 14wk Littermate WT control mice, lung_scRNA; Mus musculus; RNA-Seq
100,000-300,000 7-AAD-/CD45-/CD326- flow sorted cells were collected and submitted for single cell capture.  Cellular suspensions were loaded on a Chromium Single-Cell Instrument (10x Genomics, Pleasanton, CA, USA) to generate single-cell Gel Bead–in–Emulsions. Single-cell RNA-seq libraries were prepared using Chromium Single-Cell 3′ Library & Gel Bead Kit (version 1.1; 10x Genomics). Libraries were sequenced using Illumina's NovaSeq6000 instrument.  5000-6000 cells were captured per condition at a read depth of 50,000 reads per cells.  The sequencing reads were examined by quality metrics, transcripts mapped to reference mouse genome and assigned to individual cells according to cell barcodes, using Cell Ranger (10× Genomics). Single-cell RNA-seq libraries were prepared using Chromium Single-Cell 3′ Library & Gel Bead Kit (version 1.1; 10x Genomics).