SNU2670HR-rep
Total RNA was extracted using TRIzol reagent and verified with TapeStation RNA analysis. RNA samples with RIN > 7.0 were used to create sequencing libraries with the TruSeq Stranded mRNA Sample Prep Kit. Libraries were quantified, checked for quality, and sequenced on an Illumina NovaSeq platform. Raw reads were trimmed with Trimmomatic and aligned to the GRCh38 reference genome using HISAT2. Gene expression was quantified with StringTie, and differentially expressed genes were identified using DESeq2 (|fold change|  2, p < 0.05).