GSM9282536: MV2_21-16_120dpi; Homo sapiens; RNA-Seq
Organoids were washed in PBS and enzymatically dissociated into single-cell suspensions using Accutase or papain digestion. Cell viability (> 85 %) was verified before loading into the 10x Genomics Chromium Controller. Single cells were encapsulated in Gel Bead-in-Emulsions (GEMs) for downstream reverse transcription and barcoding. Single-cell RNA-seq libraries were prepared using the 10x Genomics Chromium Next GEM Single Cell 3′ Reagent Kits v3.1 according to the manufacturer's protocol. cDNA was amplified, fragmented, end-repaired, A-tailed, and adaptor-ligated. Indexed libraries were quantified by Qubit, quality-controlled by Bioanalyzer, pooled, and sequenced on an Illumina NovaSeq 6000 instrument.