GSM9195241: PmDay2; Patiria miniata; RNA-Seq
The embryos were collected and washed twice with calcium-free sea water, and then suspended in hyalin-extraction media (HEM) for 10-15 min, depending on the stage of dissociation. When cells were beginning to dissociate, the embryos were collected and washed in 0.5 M NaCl, gently sheared with a pipette, run through a 40 μm Nitex mesh, counted on a hemocytometer and diluted to reach the appropriate concentration for the scRNA-seq protocol. Single cell encapsulation was performed using the Chromium Single Cell kit on the 10x Genomics Chromium Controller. Single cell cDNA and libraries were prepared using the Chromium Single Cell 3' Reagent kit v3.1 Chemistry. Libraries were sequenced by Genewiz on the Illumina Novaseq.