RNA-seq of Trebouxia lynnae: several physiological treatments
An equimolar mixture of the RNA extracted from the nine conditions was used to prepare the library for sequencing. Any rRNA was removed using Epicentre ribo-depletion protocol with a Ribo-Zero rRNA Removal Kit Plant Leaf (Illumina Inc., San Diego, California, USA). The ribo-depleted RNA was fragmented by incubation for 5 min at 94C, the library was constructed using the Illumina TruSeq stranded mRNA library preparation kit following the manufacturer's instructions. Sequencing was carried out in a Illumina MiSeq benchtop sequencer with a v3 2x200 pb kit at the Sequencing Service of the Servicio Central de Soporte a la Investigacin Experimental (SCSIE) of the University of Valencia (Burjassot, Spain).