GSM8963214: A549 cells, NT, rep 5; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol reagent (Thermo Fisher), purified on Direct-zol RNA miniprep kit (Zymo Research). Libraries were prepared using after ribosomal RNA depletion (Ribo-Zero Gold rRNA Removal Kit, Illumina), fragmentated and first-strand cDNA synthesized using random hexamer primers, second-strand cDNA synthesized and remaining overhangs were converted into blunt ends. Adapoters were ligated to after adenylation of 3' ends and cDNA fragments of preferentially 370-420 bp length were purified with AMPure XP system. After PCR and quality assessment libraries were sequenced using a Illumina NovaSeq 6000 platform in 150 bp paired-end configuration to a depth of 40-50 million read pairs per sample.