Long-read Amplicon Sequencing of lDE20 Plasmid Library
Sample plasmid was miniprepped and then the cassette amplified using limited cycle PCR. PCR products were cleaned up with AMPure beads and prepped for library sequencing according to a standard ONT protocol using the ligation sequencing kit (SQK-LSK109). If necessary to achieve adequate depth, multiple sequencing runs were conducted on a single library and the reads pooled.