Sonicated, sample 1592, input
Nave B cell nuclei DNA was sonicated to ~150 bp fragments using a Bioruptor Pico. Fragmented DNA was end-repaired and adapter-ligated using the KAPA HyperPrep kit. Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit with NEBNext Multiplex Oligos for Illumina without 5hmC enrichment and used as input controls. Libraries were quality-checked using TapeStation and Qubit and sequenced on an Illumina NovaSeq 6000 at the Institute for Genomic Medicine at Nationwide Childrens Hospital (Columbus, OH).