GSM8383537: CON-hip-3; Rattus norvegicus; RNA-Seq
Total RNA was extracted by TRIzol method, and the concentration of RNA was ensured to be in the range of A260/A280 ratio of 1.8~2.1 1 - 2 μg total RNA was taken from each sample to construct sequencing library. The mRNA is purified, fragmented, and PCR amplified according to the assay. Prepare the mixed library according to the experimental design, ensure that the final concentration of the mixed library is 2 nM, and the volume is greater than or equal to 10 µL. The prepared library is stored at -80℃. Prepare the sample of the library for sequencing on