3 wk post wet-up soil metaT replicate C
RNA extraction from fresh using the Qiagen All-Prep Kit. Fast select rRNA depletion Kit (Bacteria) and NEBNext Ultra II RNA Library Prep Kit. Enriched RNAs were fragmented for 15 minutes at 94C. First strand and second strand cDNA were subsequently synthesized.  cDNA fragments were end repaired and adenylated at 3ends, and universal adapter was ligated to cDNA fragments, followed by index addition and library enrichment with limited cycle PCR (6-14 cycles).