GSM7371895: serotonin neurons, widetype; Homo sapiens; RNA-Seq
At day42 of the differentiation of TPH2-EGFP reporter hPSCs towards serotonin neurons, cells were dissociated by accutase and filtered through cell strainer (40 µm) to obtain single cells. Highly purified EGFP-labeled SNs were then derived by fluorescence-activated cell sorting (FACS). cDNA amplification and library construction were performed according to standard protocols.  Breifly, Single-cell suspension was added to the 10x Chromium chip according to the instructions for the 10X Genomics Chromium Single-Cell 3' kit (V3). Gel beads containing barcode information were bound to a mixture of cells and enzymes and then encapsulated by droplets of oil surfactant located in a microfluidic system to form gel bead-in-emulsions (GEMs). After GEMs flowing into a reservoir and being collected, the gel beads were lysed to release the barcode sequence. Then the cDNA fragment were reverse transcribed and the cells were labeled. The products of all GEMs were mixed and a standard sequencing library was constructed.