Platanthera chlorantha, pool of root samples
The RNA extracts of leaves and roots of the nine species were polled by species and organ to construct 18 Nanopore sequencing libraries using the cDNA-PCR sequencing kit SQK-PCS109 combined with the barcoding kit SQK-PBK004 (Oxford nanopore technologies, UK) and sequenced on R9.4 MinIon flow cells. Basecalling, demultiplexing and trimming was done using Guppy v4.0. Between 2.88 and 10.22 millions of reads were obtained.