Single cell RNA sequencing from main duct IPMN of patient 1593
Single-cell complementary DNA libraries were prepared and sequenced at the University of Michigan Sequencing Core using the 10× Genomics Platform. Samples were run using 50-cycle paired-end reads on the NovaSeq 6000 (Illumina) to a depth of 100,000 reads.  Cell Ranger count version 6.1.2 was used with default settings, with an initial expected cell count of 10,000.  The GRCh38-2020-A reference  was used for alignment