GSM8381350: retina from AAV2-3; Mus musculus; RNA-Seq
Total RNA was extracted from retina using Trizol reagent (15596018CN, Invitrogen, USA), following the manufacturer's protocol. RNA quantity and integrity were assessed using Qubit® RNA Assay Kit in Qubit® 2.0 Flurometer (Life Technologies, CA, USA) and the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Libraries for RNA sequencing were prepared using NEBNext® Ultra™ RNA  Library Prep Kit for Illumina® (NEB, USA), and sequencing was performed on an Illumina NovaSeq platform to generate paired-end reads of 150 bp.