GSM8045996: C-5_A_control_primodial_single_oocyte_RNA; Mus musculus; RNA-Seq
P10 female mice were intraperitoneally injected with PMSG. Oocytes were harvested in M2 medium 48 h after PMSG. The ovaries were digested in 500 µl HBSS supplemented with 0.25% trypsin, 1 mM EDTA, and 0.01% DNase I and incubated at 37°C for 10 min with gentle agitation. After aspirating the supernatant completely, the cells were resuspended in 500 µl HBSS. The dissociated single-cell suspensions were transferred under the microscope (Nikon, SMZ1000), and single oocyte was washed three times with 0.1% BSA/PBS, each oocyte was transferred into 0.2 ml PCR tubes containing 2 μl lysis buffer. Transcriptome libraries were prepared following the Smart-seq2 protocol (Picelli et al., 2013). Sequencing libraries were constructed by using KAPA HyperPlus Kit (Kapa Biosystems) according to the manufacturer's instructions. All libraries were sequenced on the Illumina NovaSeq 6,000 platform with 184 bp paired-end mode at the Annoroad Genomics Company (Beijing, China).