ONT sequencing of ASFV strain HK_NT_202103 using R9.4.1 flow cell
In brief, 4g of total DNA was used for the MinION nanopore library preparation using Ligation Sequencing Kit (LSK110) (Oxford Nanopore Technologies (ONT)) according to the manufacturers instructions. Sequencing was performed in a MinION sequencer with R9.4.1 flow cells (FLO-MIN106D). The DNA base calling was performed using Nanopore Guppy (v 5.0.11; ONT) with a high-accuracy model (dna_r9.4.1_450bps_sup.cfg).