GSM7656545: NK-ctrl; Homo sapiens; RNA-Seq
For the scRNA-seq analysis of human cord blood NK cells, NK cells from cord blood were sorted using the NK Cell Isolation Kit human (Miltenyi Biotec); the purity reached more than 95%. Pool 4 cells from the control group in one tube and 4 cells from the Venetoclax-treated group in another tube.We then counted and resuspended the pooled cells at a concentration of 1000 cells/µL, targeting an estimated 6000 cells per library, following the instructions of the Single Cell 3ʹ Solution v3 Kit (10X Genomics). Single-cell libraries were constructed strictly according to the manufacturer's standard protocol. Each sequencing library is generated using a unique sample index. Libraries were sequenced on the Illumina NovaSeq 6000 System.