GSM8383321: Colon,BEC, NI-3; Mus musculus; RNA-Seq
RNA was harvested from 250 sorted blood and lymphatic endothelial cells using RNA cleanup XP beads RNA was eluted for 1 min in RT-cDNA synthesis mix 1 (0.5 µl P1-T31 (20µM), 0.3 ml RNasinplus (Promega), 1.5µl 10µM dNTP, 3.5 µl 10µM Tris pH 7.5-0.5% IGEPAL CA-630 (Sigma) and 1.7 µl RNase free ddH2O) and pipetted up and down to mix. The eluted sample was then incubated for 3 min at 72º