GSM9046518: E18.5; Mus musculus; OTHER
5 um FFPE sections were cut and mounted on a positively chaged glass slide. Three sections from the same tibia were placed on the slide within 6.5x6.5 mm area, stained with Hematoxylin and Eosin, imaged on a Nikon Ti2-E inverted microscope with a 20X/0.8NA objective. The sections were processed, transferred onto Visium HD slide, and further processed to prepare cDNA library using 10X Genomics CytAssist instrument and 10X Genomics Visium HD slide and reagents kit according to the manufacturer's protocol.