Tulipa uniflora
Total genomic DNA was extracted from silica gel-dried leaf material using the CTAB method. A chloroplast genome library was prepared from the total genomic DNA using the TruSeq DNA Nano Kit along with the NextSeq 500 platform (Illumina, San Diego, CA, USA), following the manufacturers protocol. Trimmomatic v.0.36 was used to remove adapter sequences and low-quality reads to reduce bias. A base quality plot generated using FastQC v.0.11.5 was used to check the overall quality of the data and show the range of quality values for each cycle. NOVOplasty v.4.1.0 was used to perform de novo assembly using various k-mers. The assembly and orientations were confirmed using the National Center for Biotechnology Informations (NCBI) BLAST tool (https://www.ncbi.nlm.nih.gov/).