mRNA of Aedes albopictus adult
Total RNA from each sample was converted to cDNA using random priming. Genomic DNA was digested using ezDNase (New England Biolabs, Ipswich, MA) according to the manufacturers protocol. cDNA was generated using the NEB NEBNext Ultra II RNA First- & Non-Directional Second-Strand Synthesis Modules (New England Biolabs, Ipswich, MA) using NEBs protocol for degraded RNA. cDNA was cleaned using .8X Ampure XP beads (Beckman Coulter Life Sciences, Brea, CA) and eluted in 13 L nuclease-free water. cDNA was prepared for sequencing using Illuminas DNA Prep (Illumina, San Diego, CA) library preparation kit and sequenced at NECE on a NextSeq 1000 using a P2 reagent cartridge at 251 bp x 251 bp.