GSM8783536: SD-2D-2; Mus musculus; RNA-Seq
Intestinal crypts were isolated from small intestine samples by incubating them in PBS containing 10 mM EDTA at 4°C for 30 min. The crypt fractions were collected by pipetting and filtered through a 70 μm cell strainer (BD Biosciences). Library was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics)