Amplicon-seq endophytic microbiome of Micropropagated Strawberry
Genomic DNA was extracted from strawberry plant tissues using the DNeasy Plant Mini Kit following the manufacturer protocol. Bacterial communities were amplified using primers targeting the 16S rRNA V5-V7 region, and fungal communities were amplified using ITS1F and ITS2 primers. PCR products were checked on agarose gel, purified with AMPure XP magnetic beads, and indexed using the Nextera XT Index Kit. Indexed libraries were quantified with the KAPA Library Quantification Kit, pooled in equimolar amounts, and sequenced on an Illumina MiSeq platform using 2x250 bp paired-end chemistry with a 25 percent PhiX spike-in.