GSM8887937: hCO_control65D; Homo sapiens; RNA-Seq
Cerebral organoids were washed with calcium and magnesium-free DPBS and incubated with Corning Cell Recovery Solution to dissociate the polymerized Matrigel. The collected hCOs were cut into 2 to 4 small pieces using a scalpel and washed with the DPBS several times to remove the potential debris. The pieces of the hCOs were disassociated into single-cell suspension through the Neural Tissue Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach, German) Following the manufacturer's instruction, the single-cell RNA sequencing (scRNA-seq) libraries were prepared with the Chromium Single Cell 3' Library & Gel Bead Kit v.3.1 (10x Genomics). The constructed libraries were sequenced following the specific procedures (R1:28 cycles, R2: 91 cycles, and i7 index: 8 cycles) by a sequencing system (NovaSeq 6000, Illumina, San Diego CA).