RNA-Seq of mus musculus: adult male lung
Total RNA was isolated from lung tissues using TRIzol reagent (Thermo Fisher Scientific). The concentration and quality of each sample were determined by an Agilent RNA 6000 nano kit in the Agilent 2100 bioanalyzer (Agilent Technologies). To construct complementary DNA (cDNA) libraries, the mRNA of each sample was selected using Oligo (dT) magnetic beads. The enriched mRNA is fragmented and reverse-transcribed into cDNA. The cDNA is subsequently amplified by adding sequencing adapters. All the sequencing of these cDNA libraries was performed on an illumina platform according to the manufacturers standard protocol.