RNAseq of mus musculus: GRb-Ad Dex Liver
mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. The mRNA was fragmented and the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The cDNA library was then processed using end repair, A-tailing, adapter ligation, size selection, amplification, and purification and then quality control was performed prior to sequencing via Illumina platform.