GSM9128146: Mouse tumor, EZH2knockout, biol rep1 (H3K27me3 ChIP); Mus musculus; ChIP-Seq
Tissue samples were first minced and resuspended in ice-cold PBS with protease inhibitor cocktail before further being disaggregated with a dounce homogenizer, then filtered through a 70mm filter to collect a single cell suspension. Collected cell pellets were resuspended in PBS containing 1% of formaldehyde for 10 min and the cross-linking reaction was quenched with glycine. Libraries were generated with NEBNext Ultra II DNA Library Prep kits (NEB, E7103L)