Hi-C sequencing of Cephalopholis sonnerati liver
The genomic DNA isolated from liver tissue was used for Hi-C sequencing. The Hi-C library preparation involved crosslinking cells with formaldehyde, digesting restriction enzymes, marking the DNA ends with biotin-14-dCTP, performing blunt-end ligation of crosslinked fragments, religating the proximal chromatin DNA, reversing the crosslinking with proteinase K, purifying, shearing DNA to a size of 200-600 bp, repairing ends, adding A-tailing, and then adding sequencing adapter. At last, the Hi-C library was sequenced on DNBSEQ-T7 platform (MGI Tech Co., Ltd., China).