GSM9420236: Bos taurus, Control_1; Bos taurus; RNA-Seq
According the manufacturer's requirement, RNA sample was extracted with a RNeasy Plus Micro Kit (QIAGEN, Dusseldorf, Germany). Total RNA was reverse transcribed using a reverse transcription kit (Biosharp, Beijing, China). The SMART-Seq v4 Ultra Low Input RNA Kit (Clontech, Japan) was used to prepare the low-input library following the procedures below: 1) Preparing cDNA library; 2) cDNA Purification and size selection (150-300bp); 3) Preparing sequencing ready library (add i5/i7adaptor using PCR method); 4) Library quality control (assess the size and concentration of the library using Agilent 2100 Bioanalyzer ); 5) Library sequencing performed on Illumina platforms using a 2x150bp paired-end sequencing protocol.