GSM8385696: Control mice, rep 1; Mus musculus; RNA-Seq
RNA was extracted from the samples using the Trizol method followed by RNA isolation using the RNeasy micro kit. RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (# E7760) combined with NEBNext Poly(A) mRNA Isolation Module (NEB #E7490) Library preps were started with 25 ng input and 14x cycles were used in the indexing PCR. Library pool was sequenced as 1.3 pM with 1% PhiX in NextSeq 500 high output 1x75bp flowcell.