GSM9598143: Polyp_ECRS_CD4_CD45ROposi; Homo sapiens; ATAC-seq
PBMCs were isolated from peripheral blood using density gradient centrifugation. Nasal polyp tissues were mechanically and enzymatically dissociated into single-cell suspensions. CD4 memory T cells were enriched or sorted (e.g., CD3⁺ CD4⁺ CD45RO⁺) using fluorescence-activated cell sorting (FACS) or magnetic bead-based separation. Nuclei were isolated using a lysis buffer optimized for ATAC-seq, followed by filtration and counting prior to library preparation. scATAC-seq libraries were prepared using the 10x Genomics Chromium Single Cell ATAC platform according to the manufacturer's instructions. Briefly, isolated nuclei were subjected to Tn5 transposition, followed by droplet-based barcoding, PCR amplification, and library construction. Libraries were sequenced on an Illumina platform to generate paired-end reads.