GSM8436250: HDCKO mouse 2; Mus musculus; RNA-Seq
The total RNA of muscles 3 days after surgery from WT and Hdc-/- mice was extracted using TRIzol reagent (Takara) for RNA-seq analysis. RNA purity and quantification were evaluated using the NanoDrop 2000 spectrophotometer (Thermo Scientific, USA). RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Then the libraries were constructed using VAHTS Universal V6 RNA-seq Library Prep Kit according to the manufacturer's instructions.