DNA-Sequencing of Salivary Gland Cancer tumors
A minimum of 100 ng of genomic DNA from both tumor and adjacent normal tissues was used for library preparation with the Rubicon DNA Thruplex kit, following the manufacturers instructions. Targeted capture was performed using a custom Nextera library with 7,814 probes covering the coding exons of 233 genes. Sequencing was conducted on an Illumina NovaSeqXPlus system with paired-end 151 bp reads.