GSM8502653: miR-186+100 nM replicate 3, 12 week; Homo sapiens; RNA-Seq
Total RNA was isolated at two time points (12 and 29 weeks) using mirVanaTM RNA isolation kit as established in our laboratory (Ferragut Cardoso et al., 2022) The Truseq Stranded mRNA kit was used to prepare mRNA libraries from 1 μg total RNA. Libraries were validated on the Agilent 2100 Bioanalyzer and quantitated using the Illumina Library Quantification Kit. All samples were pooled and run simultaneously, using 2 × 150 paired end sequencing with the 500 High-output v2 (300 cycles) sequencing kit on the Illumina NextSeq500 instrument.