GSM9843382: Mouse ovary immune cells PCOS TCR; Mus musculus; OTHER
Ovaries were collected from control and PCOS model mice and dissociated into single-cell suspensions. Ovarian immune cells were enriched or sorted according to the experimental design. Cell viability and concentration were assessed before single-cell library preparation. Single-cell gene expression libraries were prepared using the 10x Genomics Chromium Single Cell 5' Gene Expression platform. Single-cell TCR V(D)J libraries were prepared from the same cell suspensions using the 10x Genomics Chromium Single Cell V(D)J enrichment workflow according to the manufacturer's instructions.