16S-seq of rat metagenome: adult Sprague-Dawley rat heart
The full-length 16S rRNA gene was amplified by PCR using barcode-labeled primers 27F (5-AGRGTTYGATYMTGGCTCAG-3) and 1492R (5-RGYTACCTTGTTACGACTT-3). Libraries were prepared using the SMRTbell Prep Kit 3.11, and paired-end sequencing was performed on a PacBio Revio platform.