RNA-Seq of canis lupus familiaris: canine aorta
RNA-seq isolates total RNA from samples, enriches mRNA via poly-A selection or depletes rRNA, then fragments RNA. Reverse transcription synthesizes cDNA, which is ligated with adapters, amplified by PCR, and sequenced on high-throughput platforms. Bioinformatic tools align reads to a reference genome or assemble transcripts to quantify gene expression and identify alternative splicing.