GSM9744926: E12.5+18h ureter cultures, control, repl. #2; Mus musculus; RNA-Seq
Extraction of total RNA was performed using the QIAGEN RnNeasy micro kit according to the manufacturer's recommendations. The total RNA samples were sent to Novogene, Munich for quality control, library construction, sequencing and bioinformatics analysis. Libraries were prepared using Illumina TruSeq Stranded mRNA Library Prep Kit following manufacturer instructions. PolyA-selected RNA was fragmented and reverse transcribed.