transcriptomic
RNA integrity was assessed using the RNA Nano 6000 Assay Kit on the Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). mRNA was enriched from total RNA and used to construct complementary DNA (cDNA) libraries. Polymerase chain reaction products were purified using the AMPure XP system, and library quality was evaluated. Indexed libraries were clustered on a cBot Cluster Generation System using the TruSeq PE Cluster Kit v3-cBot-HS (Illumina), in accordance with the manufacturers instructions. After cluster generation, libraries were sequenced on the Illumina NovaSeq platform to produce 150 bp paired-end reads. Raw sequencing data in FASTQ format were processed using in-house Perl scripts.