Bacterial community composition of Atacama camp permafrost active layer
The PCR was performed using Bacteria-specific (Bakt_341F: 5-CCTACGGGNGGCWGCAG-3 and Bakt_805R: 5-GACTACNVGGGTATCTAATCC-3) (Herlemann et al. 2011) and Archaea-specific (A519F: 5-CAGCMGCCGCGGTAA-3 and Arch855R: 5-TCCCCCGCCAATTCCTTTAA-3) (Klindworth et al. 2013) primers targeting the V3-V4 variable regions of the 16S rRNA gene.