GSM9597756: mesenteric lymph nodes single cells from Bhlhe40 ko mice; Mus musculus; RNA-Seq
Mesenteric lymph nodes(MLNs) were enzymatically digested in R10 media containing 100U/ml type VIII collagenase and 150ug/ml DNase I for 30 min at 37°C.CD45+MHCII+Lineage- cells were isolated from the MLNs. Single-cell RNA-seq libraries were generated using the 10x Genomics Chromium Single Cell platform according to the manufacturer's instructions.