RNA-Seq of Amaranthus tuberculatus
Total RNA was extracted using a TRIzol-based protocol, and each sample was treated with DNase I to remove genomic DNA contamination. RNA quality was checked on a 1% agarose gel, and RNA concentrations were measured using a Qubit 4.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA). Libraries were sent to the Roy J. Carver Biotechnology Center, University of Illinois and sequenced on an Illumina NovaSeq X Plus 10B platform, producing 150-bp paired-end reads.