Tagmentation-based tag integration site sequencing of Nicotiana benthamiana transformed with R2Tg ribonucleoprotein constructs for DNA insertion: Replicate 3
Genomic DNA was mixed with Tn5 Transposase loaded with adaptors. Tagmented samples were purified and amplified with a nested PCR using insert specific primers and primers targeting the Tn5 adaptors. Amplicons were indexed prior to multiplexed sequencing.