V3V4 Region of 16s Gene: Sample 5
The amplicons from each sample were decontaminated with Ampure beads to remove unexploited primers and further 8 cycles of PCR were performed using Illumina barcoded adapters to prepare the sequencing libraries. Libraries purification was done using Ampure beads and quantitated through Qubit dsDNA high sensitivity assay kit. Sequencing was executed using Illumina Miseq administered with 2x300PE v3 sequencing kit.