Illumina sequencing of PCR amplicons spanning the 3' insertion junction, from Nicotiana benthamiana transformed with R2Tg ribonucleoprotein constructs for DNA insertion: Replicate 2
Genomic DNA was PCR amplified using a primer specific to the 3_ end of the inserted DNA and a primer specific to N. benthamiana rDNA. Amplicons were sequence by the Massachusetts General Hospital CCIB DNA Core (Harvard).