RNA-Seq of Apis mellifera ligustica
Total RNA is isolated from the bee in the difference phase. Depending on the research focus, specific RNA types may be enriched or depleted. RNA is often fragmented to a suitable size range. It is then reverse-transcribed into complementary DNA (cDNA). During library construction, adapters are added to the cDNA fragments to facilitate sequencing on NGS platforms. Maintaining strand-specific information during this step is crucial for accurately identifying overlapping genes and novel transcripts.