RNA-seq of brain tissue from the subventricular zone, dentate gyrus and nucleus accumbens of adult male and female voles undergoing pair bond formation
RNA-seq of brain tissue from the subventricular zone, dentate gyrus and nucleus accumbens of adult male and female voles undergoing pair bond formation
Subjects were euthanized with an overdose of pentobarbital (6.3 mg/vol) by intraperitoneal (IP) administration, and the whole brains were obtained to dissect the SVZ, DG, and NAc tissues. Briefly, whole brains were collected in Petri dishes with an ice-cold solution of 1X PBS, 2 mM HEPES, 20 mM glucose, and 25 mM NaHCO3. The three brain regions were dissected under a stereoscope and immediately frozen. Total RNA was isolated from each sample using TRIzol and DNase I.RNA integrity was assessed using a Agilent BioAnalyzer, retaining only samples with an RNA integrity number (RIN) >8.0.  Libraries were constructed using the Truseq Stranded mRNA library prep kit  according to the manufacturerâ€™s instruction (Illumina). 