GSM8677818: human_sg12_2; Homo sapiens; OTHER
During the hip replacement surgery, the human femoral head samples were collected and temporarily stored at 4 °C before being transferred to the wet laboratory at Tulane School of Medicine. Samples, measuring approximately 1 cm × 1 cm × 0.5 cm, were excised from a location close to the central area of the femoral head and immediately fixed in 10% formalin and placed on the shaker at 4 °C for 48 hours, during which the reagent was changed every 12 hours. Subsequently, the samples were rinsed with sterile PBS and decalcified in 14% EDTA for 3 weeks on a shaker, with a fresh reagent change every 3 days. The decalcified samples were dehydrated by immersion in increasing concentrations of ethanol. The dehydrated samples were then cleared by Xylene. The samples were embedded in paraffin and stored for future use. Spatial transcriptome sequencing libraries were prepared using the 10x Genomics Visium Spatial Gene Expression Slides & Reagent Kit according to the manufacturer's instructions.