GSM9455362: 3D_MDA-MB-231_replicate 2; Homo sapiens; RNA-Seq
For 2D and 3D cultures, total RNA was extracted from the cell lines using Quick RNA Miniprep Kit from Zymo Research (Irvine, CA, USA) and sent to Azenta (TU-BcX-4IC) (Burlington, MA, USA) for RNA sequencing. BA-MaPS (adipose tissue) samples were extracted using Qiazol and then Quick RNA Miniprep Kit from Zymo Research (Irvine, CA, USA) following tissue extraction protocol. The RNA sequencing workflow after RNA isolation included initial PolyA selection: based mRNA enrichment, mRNA fragmentation, and random priming with subsequent first- and second-strand complementary DMA (cDNA) synthesis. Afterward, end-repair 5' phosphorylation and nucleotide (dA)-tailing were performed. Last, adaptor ligation, polymerase chain reaction (PCR), and Illumina NovaSeq technology-based sequencing with 2 150-base pair (bp) read length were carried out.