Vreelandella zhaodongensis BS253 cultured in NaHCO3 for 12 h, biological replicate 2
Vreelandella zhaodongensis BS253 was cultivated in minimal salts medium (MSM) under three experimental conditions: basal MSM without inorganic carbon supplementation (control), MSM under a 10% CO2-enriched atmosphere, and MSM supplemented with 2% NaHCO3. Cultures were incubated at 28 C with shaking at 180 rpm. Samples were collected at 12 h and 18 h of cultivation, with three biological replicates per condition. Total RNA was stabilized in RNAlater prior to RNA extraction. RNA-seq libraries were prepared following rRNA depletion and sequenced on an Illumina NextSeq 2000 platform.