amplicon sequencing of fungi from a negative control
Genomic DNA was extracted with the Qiagen DNeasy PowerSoil Pro Kit and used for PCR in duplicate with 5.8S-Fun/ITS4-Fun primers to generate ITS amplicons. Duplicate PCR reactions were pooled after agarose gel electrophoresis confirmed successful PCR amplification for both replicates. Amplicons were sent to the Cornell BRC for sequencing with a v2 500 bp kit with 2x250 Paired Ends on the Illumina Miseq.