GSM8852318: ATAC_NSC-rep2; Drosophila melanogaster; ATAC-seq
Larval brains were dissected and dissociated into single cells, then the cells were arrested by ConA beads, and the proteins of interest were combined by the specific primary antibody, followed by secondary antibody and Tn5 transposase. To obtain interphase and mitosis cells, the dissociated cells were fixed with 0.5% formaldehyde for 8min and quenched by Glycine., then stained with pH3 (phospho-histone H3) and DAPI before ConA beads incubation. ATAC-seq libraries were prepared using Hyperactive ATAC-Seq Library Prep Kit for Illumina (Vazyme). 1w-9w cells were used as starting materials. Libraries were amplified using 14 cycles on the thermocycler. 200-2000 bp DNA was purified to construct library.