RNA-seq of Bone Marrow Mesenchymal Stem Cells:adult female bone marrow
Total RNAs from aged BMMSCs (P10-12) with/without em-eNMs were extracted by Trizol. Libraries were prepared by NEBNext kit, sequenced on Illumina HiSeq 3000, QC by FastQC/FASTX, mapped by HISAT2, and DEGs analyzed by Ballgown (1.5-fold, P<0.05).