GSM8337674: RNA-seq MDA-MB-436_untransfected_NHE1_KO_Rep2; Homo sapiens; RNA-Seq
RNA was extracted with the RNeasy Mini kit (Qiagen, 74104) according to manufacturer instructions with sample concentrations assessed via NanoDrop Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dTTP for non-directional library