Untreated female hemocyte
Healthy female adult locusts were treated with tween water. The arthrodial membrane of the hindlegs of treated adults was swabbed and air-dried for 15 min for the collection of hemolymph. After cutting the hindleg, female adult hemolymph was collected using a micropipette and transferred to Trizol reagent (Invitrogen, U.S.A). RNA was extracted using the Trizol Reagent per the manuals instructions. Extracted RNA were sent to company Illumina sequencing. A total of four replicates of treated individuals were performed for the female haemolymph collection at each time interval. Hemolymph was collected from 1015 female adults. A total of five boxes were considered one replicate for hemolymph extraction.