WGS of human gastric adenocarcinoma
DNA was extracted from FFPE blocks or fresh frozen (FF) tissues using the ReliaPrep FFPE gDNA Miniprep System (Promega), the QIAamp DNA Mini Kit (Qiagen), and the QIAamp DNA Blood Mini Kit (Qiagen) for FFPE, FF tissues, and blood, respectively. SureSelect libraries were prepared according to the manufacturers instructions. The resulting libraries were then sequenced on the NovaSeq 6000 platform (Illumina, San Diego, CA, USA) with 151 bp paired-end configuration. Tumor specimens were sequenced at an approximate depth of 60X, while normal samples were sequenced at an approximate depth of 30X.