GSM9744686: ileum, A355, Model; Mus musculus; RNA-Seq
After 19 days of OEA treatment, fresh ileum samples from control, TNBS-treated, and OEA-treated mice were collected, preserved in MACS® Tissue Storage Solution, and analyzed by single-cell RNA sequencing Ileum samples were collected from mice in control, TNBS-treated, and OEA-treated groups (n=4 per group). Immediately after dissection, tissues were preserved in MACS® Tissue Storage Solution at 4°C. Single-cell suspensions were prepared from each sample following enzymatic digestion and filtration. Cell viability and concentration were assessed prior to library construction. Single-cell encapsulation, mRNA capture, and barcoding were performed using the DNBelab C4 microfluidic platform according to the manufacturer's protocol. Reverse transcription and cDNA amplification were completed in droplets. Sequencing libraries were constructed and subjected to high-throughput sequencing on a DNBSEQ platform.