RNA-Seq of dog cutaneous melanoma
The library was prepared in accordance with the Illumina TruSeq Stranded Total RNA Sample Preparation Guide (Part # 15031048, Rev. E, Date October 2013) for Illumina Paired-End Indexed Sequencing. The first step involves the removal of ribosomal RNA (rRNA) using biotinylated, target-specific oligos combined with Ribo-Zero rRNA removal beads. Following purification, the samples were not fragmented because the Rna Integrity Number of starting material was very low.