GSM9421121: CD1, E17, nephrogenic zone cell; Mus musculus; RNA-Seq
Nephrogenic zone cells (NZC) were isolated from pooled E17 wild-type CD1 kidneys following a 15 minute incubation at 37oC with a solution containing 0.25% pancreatin and 0.1% collagenase in DPBS. NZCs were quenched with FBS at 10% volume following digestion. NZCs were resuspended to 106 cells/mL in DPBS and kept on ice prior to mRNA capture. The Chromium GEM-X Single Cell 3' Kit v3 (10x Genomics) was used following the manufacturer's protocol for mRNA capture and cDNA synthesis. mRNA capture and cDNA synthesis was performed using the Chromium GEM-X Single Cell 3' Kit v3 (10x Genomics) following the manufacturer's protocol. cDNA was then amplified, fragmented, and subjected to 3' library construction with unique sample dual-indices. Dual-indexed libraries were diluted, pooled in equal molarity, and sequenced in Illumina NovaSeq6000 on a SP-100 flow cell following 10X Genomics' recommendations for paired-end sequencing.