GSM8853082: Condition fat pad tumor. Cell Line  YEJH-FP3. Rep n2; Mus musculus; RNA-Seq
For PyMT+ cell lines, cells lines were previously generated by Gounis et al., (Gounis et al, 2024), and plated at 0.5x106 - 1x106 cells per 10cm dish, 37°C/5% CO2 for 24 hours to achieve 80% confluence the next day. Cells were washed twice in ice-cold PBS, lysed into 1mL Trizol, and RNA prepped as manufacturer instructions. RNA 6000 Pico Kit and Bioanalyzer (Agilent Technologies) was used to assess RNA integrity, sample library preparation was performed using the Illumina TruSeq RNA Library Prep kit v2, and sequencing was conducted on the NextSeq500 platform using a High Output 75 cycle kit.