GSM8717159: AT2 cells, 6 hours, 8 ug/cm2 Diesel exhaust PM, replicate 3; Homo sapiens; RNA-Seq
Following PM exposure, cells were washed with 1 ml sterile phosphate-buffered saline (PBS) and lysed with 300 µl RNA lysis buffer (Monarch® Total RNA Miniprep Kit, New England BioLabs, Massachusetts, USA) for 10 min to completely lyse cells. Lysates were stored at − 80 °C until further use. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification