RNA-seq of mouse distal colon treated with prednisolone, rapamycin or both in dextran sodium sulphate (DSS)-induced colitis
RNA-seq of mouse distal colon treated with prednisolone, rapamycin or both in dextran sodium sulphate (DSS)-induced colitis
Distal colon tissue was dissected and washed with cold PBS, then preserved in RNA later (Merck) until RNA extraction. Total RNA was extracted using RNeasy mini kit (Qiagen), following the manufacturer's instructions. 'Illumina stranded Total RNA Prep, Ligation with Ribo-Zero Plus e Illumina stranded mRNA Prep, Ligation' protocol was followed.