Y1H
A yeast one-hybrid (Y1H) library was constructed using the MATCHMAKER One-Hybrid System (Clontech, Takara Bio., Japan; Catalog #K1603-1) following the manufacturers instructions. The pHIS2 bait vectors with TaSLC25A4-7B promoter was transfected into Y187 yeast cells, followed by transformation with prey vector pGADT7.