ATAC-seq in ZFP91-kockout K562 (ZFP91-KO2)
"Lysed cells were treated with Tn5 transposase preloaded with Illumina-compatible sequencing adapters (Diagenode #C01070012), and DNA was extracted using NucleoSpin Gel and PCR clean-up (Takara Bio #740609). Each transposed sample was then amplified by PCR using NEBNext Ultra II Q5 (New England Biolabs #M0544) and primers with uniquely attached index sequences. The PCR products were subsequently purified using Agencourt AMPure XP (Beckman Coulter #A63880)."