GSM9127226: P1112; Homo sapiens; RNA-Seq
Total RNA was isolated using mirVana RNA Isolation Kit (ThermoFisher). RNA concentration was measured using Qubit RNA BR assay kit (ThermoFisher). RNA integrity was checked using RNA 6000 Nano chip read by Aligent 2100 bioanalyzer. RNA-seq libraries were prepared using either the TruSeq Stranded Total RNA Sample preparation kit with Ribo-Zero Gold (Illumina) by IGM Genomics Center at UC San Diego, or the KAPA RNA HyperPrep Kit with RiboErase (Roche).