GSM8887975: Kidney_IXA62-4; Mus musculus; RNA-Seq
For mouse tissue experiments, 4-5 biological replicates were studied for each treatment condition. Total RNA from liver or kidney was isolated using Zymo Research Direct-zol RNA Miniprep kits following the manufacturer's instructions after homogenization in TRIzol reagent. For HEK293 cells, 3 biological replicates were studied for each treatment condition.Total RNA from HEK293 cells were isolated using QuickRNA mini kit (Zymo) according to the manufacturer′s instructions. Whole transcriptome RNA sequencing was conducted by BGI Americas on the BGI Proprietary platform. libraries were constructed at BGI