GSM9370090: Single-cell RNA-seq of c-Kit+ HSPCs from KrasG12D/+ mouse (JMML); Mus musculus; RNA-Seq
Single-cell suspensions were prepared from enriched C-Kit⁺ HSPCs according to the manufacturer's instructions for 10x Genomics Chromium Single Cell 3′ v3 kits. Single-cell RNA-seq libraries were generated using the 10x Genomics Chromium Single Cell 3′ v3 kits following the manufacturer's protocol. Briefly, cells were resuspended in master mix and loaded together with gel beads and partitioning oil into the Chromium chip to generate Gel Beads-in-Emulsion (GEMs). Poly-A RNA from individual cells was reverse transcribed into cDNA containing Illumina R1 primer sequence, Unique Molecular Identifiers (UMIs), and 10x barcodes. The pooled barcoded cDNA was cleaned using Silane DynaBeads, amplified by PCR, and size-selected using SPRIselect reagent for library construction. During library preparation, Illumina R2 primers, P5 and P7 sequences, and sample indices were added.