GSM9229305: Female_mice_F585_5XFAD_Trem2_T96K_p; Mus musculus; RNA-Seq
Microglial cells (CD11b+CD45Low-to-Intermediate) were collected into PBS (pH 7.4) containing 1mM EDTA based on FACS sorting using the FACSAria Fusion machine (BD Biosciences). Library preparation was performed following the manufacturer's instructions (#PN-120237, 10× Genomics). Briefly, the single-cell suspension and ChromiumTM Single Cell 3' v2 Master Mix were loaded into ChromiumTM Single Cell A chip to collect individual cells encapsulated by gel bead-in-emulsion (GEMs) using the 10× ChromiumTM Controller apparatus. Quality control of the cDNA library was examined with the High Sensitivity DNA Kit (#5067-4626, Agilent Technology) on the Bioanalyzer 2100. Library sequencing was performed in paired-end mode on Illumina NextSeq 2000 instrument to the depth of approximately 100 million reads per library at MGH Next-Generation Sequencing Core.