RNA-seq data of wildtype and ZNF334-edited THP-1 cell lines with or without cold stimulation
RNA-seq data of wildtype and ZNF334-edited THP-1 cell lines with or without cold stimulation
THP-1 cell lines (ZNF334-wild-type, ZNF334+/−, and ZNF334−/−) were seeded onto 24-well imaging plates (Greiner). For cold stimulation assays, cells were exposed to 32°C for 8 hours in the incubator. Total RNA was isolated from cultured cells using the Qiagen Total RNA Kit according to manufacturer's instructions. Library were constructed by the Qubit fluorometer with Qubit RNA BR/HS Assay Kit and sample integrity was analyzed by assay method: Agilent 4150 TapeStation system with RNA/High Sensitivity RNA ScreenTape.