amplicon of Felis catus: urine
DNA was extracted from each urine sample using ZymoBIOMIC -96 MagBead DNA kit (Zymo Research, Irvine, CA). For library preparation, the extracted samples were processed with Quick-16S NGS Library Prep Kit (Zymo Research, Irvine, CA). In the step of 16S rRNA gene amplification, the Quick-16S Primer Set V3-V4 (Zymo Research, Irvine, CA), was used in the PCR reactions.