RNA seq of HL-60 cells in untreated, ATRA (All- trans retinoic acid) and Vitamin D3 treated conditions
RNA seq of HL-60 cells in untreated, ATRA (All- trans retinoic acid) and Vitamin D3 treated conditions
Leukemic cell line HL-60 was procured from ATCC and maintained in IMDM supplemented with 20% FBS. HL60 cells were cultured in IMDM media supplemented with 20% FBS. HL60 WT cells were induced with 10µM ATRA (All Trans Retinoic Acid) and 50nM Vit-D3 (Vitamin D3) for 72 hours. Total RNA was extracted using Qiagen RNeasy kit using manufacturer's protocol. Quality and concentration of extracted RNA was checked using Qubit BR RNA kit and Agilent screentape respectively.   Library was constructed using Trueseq stranded sample preparation kit from Illumina using Ribo-zero.