GSM8386695: Hela 1; Homo sapiens; RNA-Seq
RNA was collected using RNeasy mini plus kit (Qiagen). A total amount of 2 μ g RNA per sample was used as input material for the RNA sample preparations. Firstly, ribosomal RNA was removed and rRNA free residue was cleaned up by ethanol precipitation. the linear RNA was digested with 3 U of RNase R (Epicentre, USA) per µg of RNA, and then used for the construction of sequencing libraries. The sequencing libraries for RNA-Seq were generated by NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer' s recommendations.