High throughput amplicon sequencing of particles-associated  bacterial diversity, in various environments potentially hosting Ca. Babelota
Nucleic acids were simultaneously subjected to qPCR and barcoding using Zymo Quick-16S Plus NGS Library Prep Kit (V3-V4, UDI) accdording to manufacturer's recommendations. The quantified amplicons were subjected to sequencing on an Illumina Miseq using 2x300 cycles