GSM9843021: sRNA_con2_Minus_PNK; Homo sapiens; ncRNA-Seq
small RNA seq: Total steady-state small RNA was purified from equal numbers (~2 million cells per small RNA-seq experiment) of THP-1 monocytes using the mirVana™ PARIS™ RNA and Native Protein Purification Kit (Catalog# AM1556, Invitrogen) according to manufacturer's protocol for sRNA enrichment . The small RNA were treated with or without Antartic Phosphatase and PNK and libraries were constructed with the NEBNext Small RNA Sample Prep kit (NEB).