GSM9668721: 8505c_shCtrl+dabrafenib_1; Homo sapiens; RNA-Seq
RNA were extracted using RNAeasy kit (Qiagen) We used 230 ng of total RNA samples to generate the library (Poly A selected) with 12 PCR cycles. The kits used for the library prep were NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490), NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760) and NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs, NEB, E6440) as described in the manufacturer's manual. Sequencing was carried out in the Illumina NextSeq2000 instrument with NextSeq™ 2000 P4 XLEAP-SBS™ Reagent Kit (200 Cycles) 101x101 pair end configuration. The demultiplexing was carried out with the illumina DRAGEN BCL convert software version 4.2.7.