DNA-Seg of broiler's caeca
Briefly, genomic DNA samples were polymerase chain reaction (PCR) amplified using barcoded universal primer pair 27F and 1492R (12x 27F barcoded primers and 8x 1492R barcoded primers), targeting the V1 -V9 region of the bacterial 16S rRNA gene. The resulting barcoded amplicons were individually quantified, pooled equimolar and ampure PB bead-based purification step was performed on the pool. The pooled sample was then used to prepare SMRTbell library following manufacture protocol starting at step 3-Pool and Cleanup, page 9 of the protocol (link below). The SMRTbell library was qualified using Fragment Analyzer (Agilent) and quantified using Qubit HS dsDNA Assay (Thermo Fisher).