GSM9420413: ageing Muscle Satellite Cells, hMG53, Rep3; Mus musculus; RNA-Seq
Total RNA was extracted from the collected cells using TRIzol reagent following the manufacturer's instructions. RNA quality and integrity were assessed using an Agilent Bioanalyzer 2100. RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit with Poly(A) mRNA Magnetic Isolation Module, following the "Low Input" protocol according to the manufacturer's instructions.