RNA-Seq of Spuriopimpinella brachycarpa: Wild (YS) habitat petiole replicate 2
Total RNA was extracted using the TransZol Up Plus RNA Kit (TransGen Biotech, Beijing, China) according to the manufacturers protocol. RNA integrity was assessed by agarose gel electrophoresis, while concentration and purity were measured with a NanoDrop spectrophotometer. Only samples meeting quality thresholds were used for subsequent library construction. cDNA libraries were prepared with the TruSeq RNA Library Prep Kit (Illumina, San Diego, CA, USA) and sequenced on the Illumina NovaSeq 6000 platform to generate paired-end reads