GSM8710057: WT3; Mus musculus; RNA-Seq
Animals (C57BL wild-type) were acquired from Beijing Vital River Laboratory Animal Technology Co., Ltd. The mice (4 weeks) were housed (three mice/cage) in a controlled (22 ℃, 45±10% humidity, 12 h light/dark cycle) environment. All animal-related experiments were conducted as per the guidelines and regulations of the care and use of laboratory animals and received approval from the animal center of Zaozhuang University (Shandong, China). The control group (n=3) was not exposed to any irradiation. The irradiated group (n = 3) received X-ray irradiation at 25 ºC with a dose of 6 Gy (800 cGy/min, radiation distance 40 cm). Following a 24-hour period，all mice were euthanized by intraperitoneal (IP) injection of sodium pentobarbital with the dose of 50 mg/kg and their thymus tissues were collected, and rapidly frozen in liquid nitrogen. The tissues were processed for RNA isolation using the TRIzol method. These RNA samples were transported on dry ice to an external company (Genechem Experimental Department) for sequencing. The library was constructed using the NEBNext® Multiplex Prep kit Illumina® and the UltraTM RNA Library Prep Kit (NEB, USA).