GSM9633942: Volunteer 9, photoexposed skin, noon (T12); Homo sapiens; RNA-Seq
RNA was extracted and purified using QIAsymphony RNA (Cat no. 931636; Qiagen, Hilden, Germany) 100ng of RNA per sample was used for library preparation, performed by polyA selection-based mRNA enrichment, mRNA fragmentation, and random priming with subsequent first- and second-strand complementary DNA (cDNA) synthesis. Following that, end-repair 5′ phosphorylation and adenine nucleotide (dA)-tailing was performed. Last, adaptor ligation, polymerase chain reaction (PCR) enrichment, and Illumina NovaSeq technology–based sequencing was performed with 20M reads per sample using Trimmomatic v.0.36.