GSM9697882: Diabetic_D3; Mus musculus; RNA-Seq
Total RNA from each sample was assessed for integrity by agarose gel electrophoresis, and quantified and further quality-checked using a NanoDrop ND-1000. For each sample, 1–2 μg of total RNA was used for sequencing library construction. Total RNA was  subjected to mRNA enrichment using the NEB Next® Poly(A) mRNA Magnetic Isolation Module. RNA-seq libraries were constructed from total RNA after oligo(dT) enrichment (or rRNA depletion) using the KAPA Stranded RNA-Seq Library Prep Kit (Roche).