GSM8384817: Gy5x7_14d, scRNAseq; Rattus norvegicus; RNA-Seq
Fresh rat esophageal tissues from the 0Gy_7d, 35Gy_7d, 5x7Gy_7d, and 5x7Gy_14d groups were stored in MACS tissue storage solution at 4°C and shipped to the processing lab within 48 hours for scRNA-seq. Esophageal tissues from five rats per group were pooled for processing. Each pooled sample was finely chopped and enzymatically digested with 5 mg/mL collagenase I, 1 mg/mL DNase I, and 0.125% trypsin-EDTA in a shaker at 37°C for 60 minutes. The resulting cell suspension was filtered through a 70 μm strainer and sorted using a Dead Cell Removal Kit to eliminate dead cells. The cells were then resuspended in D-PBS containing 0.04% BSA at a concentration of approximately 1,000 cells/μL in preparation for scRNA-seq. scRNA-seq libraries were prepared using the Chromium Single Cell 3' Library & Gel Bead Kit v3.1 (10x Genomics) following the manufacturer's protocol. The final libraries were sequenced using an Illumina NovaSeq 6000 sequencer.