GSM9036614: Med14S983A_CON; Rattus norvegicus; ATAC-seq
Nuclei were extracted with hypotonic cell lysis. Tagmentation was performed on 50,000 nuclei for 30 min at 37C using Illumina Tagment DNA enzyme. DNA was isolated using Qiagen MinElute Reaction Cleanup Kit and amplified with Illumina/Nextera i5 common and i7 index adapters.