GSM9420722: OB_TNFa_OC1_1; Mus musculus; OTHER
Cells were crosslinked with 2% formaldehyde for 10 min and quenched with 125 mM glycine. Cells were washed with cold D-PBS, harvested, and pelleted (1,000 × g, 5 min). Nuclei were isolated using lysis buffer (140 mM NaCl, 1 mM EDTA, 0.5% IGEPAL CA-630, 1% Triton X-100, 50 mM HEPES-KOH pH 7.5) with protease inhibitors (cOmplete, EDTA-free; Sigma-Aldrich, St. Louis, MO, USA). Libraries were sequenced as 150-bp paired-end reads on a NovaSeq platform.