CA53PC1 (CRX1984080; CRA030165)
Take 1 microg of genomic DNA from the sample, randomly fragment it into approximately 350 bp segments using a Covaris ultrasonic disruptor, and then construct the library. The entire library preparation is completed through steps including end repair, A-tailing, adapter ligation, purification, and PCR amplification. After library construction, AATI is first used to detect the integrity of library fragments and the size of inserted fragments. Once meeting the expected requirements, Q-PCR is employed to accurately quantify the effective concentration of the library (with an effective concentration > 3 nM) to ensure library quality. After passing the library inspection, different libraries are pooled according to the requirements of effective concentration and target sequencing data volume, followed by PE150 sequencing.