RNA-seq of Ixodes scapularis larvae during Borrelia burgdorferi acquisition
Larvae were fed on mice infected with Borrelia burgdorferi. After detachment from the mice, larvae were collected, and total RNA extraction was performed with the AllPrep DNA/RNA/Protein Mini Kit (QIAGEN) according to the manufacturer's specifications. The library was constructed using the NEBNextUltraTM II (Directional) RNA with polyA selection library prep kit and sequencing was performed in an Illumina Novaseq 6000 DNA sequencer.