GSM8565577: Co-cultured, ex vivo 24h, DMSO, replicate 1; Mus musculus; RNA-Seq
Solely cultured and co-cultured ex vivo embryos were collected at 24h. Embryos were immediately dissolved in SMART-Seq HT Kit 10X Lysis Buffer (Takara Bio, Japan) and processed for RNA sequencing library preparation. In total, 24 embryos from 3 donor mice were used and divided into four groups. Consequently, 6 embryos in each group were integrated and regarded as one biological replicate. For endometrial samples, 3 ex vivo ISs from a recipient mouse were cut with micro scissors in 500 μm size and combined as one biological replicate. RNA was extracted with TRIzol RNA Isolation Reagents (Thermo Fisher Scientific, USA). RNA sequencing library was prepared using SMART-Seq mRNA HT LP (TAKARA Bio, Japan).