WES of canis lupus familiaris: adult blood
This study performed whole exome sequencing (WES) on canine hepatocellular carcinoma (HCC) tumor and matched blood samples to investigate somatic mutations and molecular characteristics of the tumor microenvironment. Total DNA was extracted using the DNeasy kit (Qiagen), and libraries were prepared using the KAPA HyperPrep Kit with IDT duplex UMI adapters. Exonic regions were captured using the Twist Alliance Canine Exome panel (Twist Biosciences) following hybridization and wash steps with IDTs XGen kit. Sequencing was performed on the Illumina HiSeq X platform in paired-end 151bp mode to achieve sufficient coverage for variant calling and downstream analysis.