GSM9099600: B37_OVAc_M116_DJ_2; Mus musculus; RNA-Seq
RNA lysate clean-up was performed using RNAClean XP beads (A63987, Beckman Coulter, CA, USA) followed by reverse transcription with Maxima H minus Reverse Transcriptase (EP0743, Thermo Fisher Scientific, MA, USA) and whole-transcription amplification (WTA) with KAPA HotStart HIFI 2 × ReadyMix (07958927001, Roche, Switzerland) for 21 cycles. WTA products were purified with Ampure XP beads (A63881, Beckman Coulter, CA, USA), quantified with Qubit dsDNA HS Assay Kit (Q32854, Thermo Fisher, MA, USA) and assessed with D5000 screentape on a 2200 Agilent TapeStation system (5067-5588, 5067-5589, Agilent, CA, USA). Diluted WTA products underwent tagmentation and indexing using the Nextera XT DNA Library Preparation Kit (FC-131-1096, Illumina, CA, USA), followed by size selection with Ampure XP beads. Size and concentration were then assessed with D1000 screentape (5067-5582, 5067-5583, Agilent, CA, USA) on 2200 Agilent TapeStation system. Libraries from four 96-well plates were pooled together and sequenced with the Illumina NextSeq 500/550 75-cycle high output kit (20024906, Illumina, CA, USA) on a NextSeq 500/550 system.