GSM9096881: GPCRGSNO2; Entamoeba histolytica; RNA-Seq
RNA of E. histolytica was extracted from ~1x10^6 cells using Trizol reagent (Ambion; Life Technologies, Grand Islands, NY, USA) based on the manufacturer's protocol. Genomic DNA was removed after treatment with DNase I (Invitrogen). At least 3.5 μg of total RNA was used for RNA-seq. cDNA was synthesized using the SuperScript III First-Strand Synthesis System (Invitrogen) according to the manufacturer's protocol.