Transcriptional effect of DLX3 transcription factor inhibition by shRNA in ETO2-GLIS2 cell lines
Transcriptional effect of DLX3 transcription factor inhibition by shRNA in ETO2-GLIS2 cell lines
GFP positive cells were sorted 48H after doxycycline induction (500 ng/mL).  Cells were lysed and RNA extract using RNeasy MiniKit (Qiagen).  cDNA libraries were synthesized from 250 ng total RNA using TruSeq Strandard mRNA Kit (Illumina). Libraries were verified for their amount and quality by capillary electrophoresis using a 2100 Bioanalyzer (Agilent Technologies).