GSM8851856: liver_3; Homo sapiens; RNA-Seq
Total RNA was extracted from formalin-fixed paraffin-embedded (FFPE) tissue samples using the High Pure RNA Isolation Kit (Roche) following manufacturer protocols. FFPE blocks were deparaffinized using xylene, followed by ethanol washes and proteinase K digestion before RNA purification using spin columns. RNA quantity and quality were assessed using NanoDrop and Agilent 2100 Bioanalyzer. RNA libraries were prepared using the NEBNext® ULTRATM II RNA Library Prep Kit (New England BioLabs) according to the manufacturer's instructions. A total of 1000 ng of RNA was used for fragmentation, cDNA synthesis, adapter ligation, and PCR amplification. Quality and quantity of the libraries were evaluated using Qubit and TapeStation before sequencing.