GSM8988766: TNFR2_WT_5; Mus musculus; RNA-Seq
Cells were lysed using the TRI reagent (Zymo, cat # R2050-1-50) and RNA was isolated using the Direct-Zol MinPrep kit from Zymo (Cat # R2050), including the optional DNaseI treatment step. RNA was eluted in 25µL RNase- and DNase-free water. mRNA was purified using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, cat # E7490). Samples were processed using the NEBNext Ultra-II RNA library prep kit from New England Biolabs (cat # E7775S) and the NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer pairs) (NEB, cat # E6440S) according to manufacturer's protocol.