T2_RKN6 (CRX2035317; CRA030663)
The V4V5 hypervariable regions of the prokaryotic 16S rRNA gene were amplified using primers 515F and 907R . Amplicons were validated via 2 agarose gel electrophoresis and purified using the Universal DNA Purification Kit to eliminate primer dimers and nonspecific products. Sequencing libraries were generated using the NEBNext Ultra II DNA Library Prep Kit for Illuminawith index sequences added. Library quality was assessed using a Qubit 2.0 Fluorometer, and sequencing was performed on the Illumina NovaSeq 6023 platform.