GSM9196902: MPMEC, H3K14la (IP); Mus musculus; ChIP-Seq
For ChIP-seq, cells were fixed with 1% formaldehyde, and nuclear genomes were extracted and subjected to immunoprecipitation (IP) according to the instructions of the kit (CST #9003). For ChIP-seq, library preparation was proceeded as follows: (1) The DNA fragments were end-repaired and A-tailed. (2) The DNA fragments with A tail were ligated with sequencing adaptors. (3) The final DNA library was obtained after size selection and PCR amplification. Libraries were analyzed for size distribution by Agilent 5400 system (Agilent, USA) and quantified by QPCR (1.5nM)