16sr RNA of feces:ALD11
The hypervariable V3-V4 regions of bacterial 16S rRNA genes were amplified using barcoded primers 341F (5'-ACTCCTACGGGAGGCAGCAG-3') and 806R (5'-GGACTACHVGGGTATCTAAT-3'). High-throughput sequencing analysis of bacterial rRNA genes was performed by the Illumina Hiseq 2500 platform provided by Biotree Co. Ltd. (Shanghai, China).