GSM9156051: SKBR3 tazemetosat + lapatinib REP 2; Homo sapiens; ATAC-seq
Nuclei were isolated from cells with >90% viability. ATAC-Seq libraries were prepared using the Omni-ATAC-Seq protocol. Isolated nuclei were subjected to Tn5-mediated transposition. Transposed reactions were cleaned with the Zymo DNA Clean and Concentrator 5 kit. Next. Illumina adapter sequences and barcodes were added to the transposed DNA fragments. Amplified libraries were purified using the Zymo DNA Clean and Concentrator 5 kit, followed by a double-sided SPRIselect Bead cleanup (Beckman Coulter). Final libraries were analyzed on a 4200 TapeStation (Agilent Technologies) and quantified via quantitative PCR using the Collibri Library Quantification Kit (Thermo Fisher Scientific).