16S rRNA gene amplicon sequencing of stool sample from Patient-012 at week 2
DNA was extracted using the DNeasy PowerSoil HTP 96 Kit (Qiagen, Cat No./ID: 12955-4), following manufacturers instructions. 16S DNA libraries were built using a 1-step PCR protocol, targeting the V4 region of the 16S rRNA gene with the 515F and the 806rcbc0 primers. Finally, paired-end reads were sequenced on an Illumina MiSeq platform in a 300 cycles format (2x150bp).