GSM9698155: 200uM genipin, rep4; Caenorhabditis elegans; RNA-Seq
Clean worm pellets were then frozen and later used for RNA extraction. Total RNA was isolated using Qiagen RNeasy micro kit (Qiagen, Hilden, Germany) following the standard protocol. Afterward, RNA was quantified photometrically with NanoDrop 2000 and stored at -80oC until use. Libraries were sequenced using Illumina HiSeq4000 sequencing platform (Illumina, San Diego, CA, USA) in a paired end read approach at a read length of 150 nucleotides.