GSM9700263: control,TCR; Mus musculus; RNA-Seq
To delineate the molecular features of E9.5-labeled γδ T cells, we performed single‑cell RNA sequencing on flow cytometry‑isolated tdTomato⁺ cells and tdTomato- from the liver of 2‑month‑old mice.Libraries were prepared using the 10X Genomics Chromium Single Cell 5' PE v3 kit and sequenced on the NovaSeq X PLUS PE150 platform. For 10X Genomics-based scRNA-seq, we implemented the Chromium Single Cell 5' PE v3 libraries according to the manufacturer's instructions (https://cdn.10xgenomics.com/image/upload/v1710231087/support-documents/CG000733_ChromiumGEM-X_SingleCell5_ReagentKitsv3_UserGuide_RevA.pdf). Sequencing was performed on the NovaSeq X PLUS pe150 platform.