GSM9698453: TSPOKO+CLP7; Mus musculus; RNA-Seq
Total ribonucleic acid (RNA) was extracted from spleen tissue samples using the NucleoSpin RNA XS (TAKARA Bio, Japan). mRNA was enriched using oligo-dT beads, followed by reverse transcription to synthesize cDNA. The synthesized cDNA was subjected to end repair and adapter ligation. Libraries were amplified by PCR, quality-checked using a Bioanalyzer 2100 system, and sequenced on the BGISEQ-500 platform.