GSM8677757: Primary Tumor 1, ECM substrate, Normoxia, Replicate 1; Mus musculus; RNA-Seq
RNA from three independent experiments was isolated using a RNeasy Plus Kit (Qiagen, Netherlands) and the RNA Integrity Number was determined with an RNA analysis kit (Agilent, Santa Clara, CA). mRNA library preparation was performed with 50ng of RNA per sample using an Illumina Stranded mRNA Prep (Illumina, San Diego, CA) following the manufacturer's instructions.