Genomic sequences of Candida auris by PromethION, ONT
For long-read sequencing, the library was prepared from 400 ng input DNA using the Ligation Sequencing gDNA - Native Barcoding Kit 24 V14 (SQK-NBD114.24, Oxford Nanopore Technologies, ONT, UK). A total of eight barcoded samples were pooled together, then loaded on the PromethION flow cell R10.4.1 (FLO-PRO114M; ONT, UK) and sequenced using PromethION P2 Solo (ONT, UK) with the default setting. Guppy (ONT, UK) v6.0.1 with the SUP (super accuracy) mode was used for base calling and quality control studies. Porechop v0.2.4 was applied to remove adapters and barcodes from the readings.