Unfed 16C individual 12 - Gill
mRNA isolated NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490L, New England Biolabs), stranded cDNA created Maxima H Minus First Strand cDNA Synthesis kit (Thermo-Fisher, USA) , all 120 fish sequenced for 150 base pair reads on one lane of a NovaSeq X