Illumina short-reads sequencing of Drosophila simulans wild-caught iso-female strain sim406
DNA was extracted using the TIANamp Genomic DNA Kit (DP304), with 1624 male flies used for each isofemale line. All libraries were prepared according to the Watchmaker DNA Library Prep Kit with Fragmentation (7K0019-096) by Annoroad Gene Technology (Beijing), and sequenced using the Illumina NovaSeq 6000 platform with paired end read length of 150 bp (PE150) by Annoroad, generating approximately 5 Gb of raw sequencing data for each strain.