single-cell CRISPR capture of K562 VPR cell gRNAs
Cells were harvested and prepared into single-cell suspensions following the 10x Genomics Single Cell Protocols Cell Preparation Guide (Manual part number CG00053, Rev C). Four lanes were used for the single-cell transcriptome profiling, with two lanes containing cells from the K562-VP64 cell line, and two lanes containing cells from the K562-VPR cell line. Roughly 10,000 cells were captured per lane of a 10x Chromium chip (Next GEM Chip G) using Chromium Next GEM Single Cell 3 Reagent Kits v3.1 with Feature Barcoding technology for CRISPR Screening (10x Genomics, Inc, Document number CG000205, Rev D).