Nanopore sequencing of PCR amplicons spanning the insertion 5 junction to assess junction type: Replicate 2
Genomic DNA was PCR amplified using a primer specific to the 5_ end of the inserted DNA and a primer specific to N. benthamiana rDNA. Oxford Nanopore sequencing of the purified amplicons was completed by Plasmidsaurus using R10.4.1 flowcells.