RNA-Seq of Mus musculus: male diaphragm muscle tissue from control group, replicate 3
Total RNA was extracted using the RNAeasy Mini Kit (Qiagen, Germany). Poly(A)+ mRNA was isolated with the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, USA). cDNA libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit, followed by purification using NEBNext Sample Purification Beads. The libraries were pooled and sequenced on the NovaSeq 6000 (Illumina, USA).