Soil bacterial diversity was measured
DNA was extracted from soil samples using a DNA kit (MN NudeoSpin 96 Soi) and the concentration was determined using NanoDrop 2000. The PCR reaction conditions were as follows: pre-denaturation at 94  for 5 min; 30 cycles of 94  for 30 s, 50  for 30 s and 72  for 64 s.