Characterization of structural variants in canine carcinoma and sarcoma
Characterization of structural variants in canine carcinoma and sarcoma
Four types of cancer tissues were collected from four different dogs. Urothelial carcinoma tissue was excised post-mortem from the bladder to the urethra of a 12-year-old female Dachshund, following confirmation of whole-body metastasis via CT. Intestinal adenocarcinoma tissue was obtained during therapeutic resection of an intestinal mass in an 11-year-old neutered male Spitz at the time of diagnosis. Nasal chondrosarcoma was diagnosed in a 5-year-old male Poodle, with tissue collected through a Tru-Cut biopsy after a CT scan of the nasal cavity. Rhabdomyosarcoma tissue was collected via Tru-Cut biopsy from a right hindlimb mass in an 8-year-old male Borzoi after anesthesia. In each case, a tissue sample measuring approximately 1 cm × 1 cm was excised, immediately stored at -80°C. Genomic DNA was extracted from each tumor tissue using a QIAamp Blood & Tissue Kit (QIAGEN, Hilden, Germany). Individual libraries were generated using Illumina TruSeq DNA PCR-Free Kit (Illumina, San Diego, CA, USA).