GSM9698565: T6-2; Mus musculus; RNA-Seq
Total RNA was extracted from hippocampal tissues of AAV-circTau6 and control-injected hMAPT mice using TRIzol reagent (Invitrogen). RNA integrity was verified with an Agilent 2100 Bioanalyzer, and all samples had RNA integrity number (RIN) > 8.0. Ribosomal RNA was depleted using the Ribo-Zero™ Magnetic Kit (Epicentre). Strand-specific cDNA libraries were prepared with the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #7530) and sequenced on an Illumina NovaSeq 6000 platform (150 bp paired-end) by Gene Denovo Biotechnology Co. (Guangzhou, China).