GSM9632886: T025_WT_3; Schizosaccharomyces pombe; RNA-Seq
RNA isolation was done using the RNeasy Mini Kit from Qiagen (Cat No. 74104) with the standard kit protocol. Messenger RNA was extracted from total RNA using poly-T oligo-attached magnetic beads. Following fragmentation, the first strand cDNA was synthesized with random hexamer primers, followed by the second strand cDNA synthesis with either dUTP for a directed library or dTTP for a nondirectional library. The non-directional library was prepared following end repair, A-tailing, adaptor ligation, size selection, amplification, and purification. The directional library was prepared following end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification.