GSM9744582: Hyperoxia female mouse 2 ATAC; Mus musculus; ATAC-seq
Nucleus were isolated from 40-50 mg of flash-frozen mouse brain tissue using the Chromium Nucleus Isolation Kit with RNase Inhibitor (PN-1000494, 10x Genomics, Pleasanton, CA, USA) according to the manufacturer's protocol, with a final nucleus resuspension volume of 100 µL. Single-nucleus partitioning was performed using a Chromium Controller (10x Genomics). snATAC-seq and GEX libraries were prepared using the Chromium Next GEM Single Nucleus Multiome ATAC + Gene Expression Reagent Bundle (PN-1000283, 10x Genomics) following the manufacturer's protocol, and libraries were sequenced on a NovaSeq 6000 Xp (Illumina, San Diego, CA, USA).