16S rRNA of top-down SynComs
gDNA was extracted following protocols from Song et al., mSystems, then DNA was submitted to SeqCenter for sequencing. The samples were sequenced for 50k reads and every sample reached >80k reads with most samples achieving >100k reads. Samples were prepared using Zymo Researchs Quick-16S kit with phased primers targeting the V3/V4 regions of the 16S gene with the following sequences: CCTACGGGDGGCWGCAGCCTAYGGGGYGCWGCAG (forward) and GACTACHVGGGTATCTAATCC (reverse). Following clean up and normalization, samples were sequenced on a P1 600cyc NextSeq2000 Flowcell to generate 2x301bp paired end (PE) reads.