plpSC_WT_REC8_CUT&Tag_rep3 (CRX825655; CRA012919)
Cells were binded to concanavalin A beads and cohesin or histones were recognized by antibodies then combined with pA/G-Tn5. DNA binds to cohesin or histones were digested by pA/G-Tn5 and released into suspension after treated with Mg2+.Extracted DNA was used to construct libraries with the Hyperactive Universal CUT&Tag Assay Kit for Illumina pro(TD904) , and qualified libraries were sequenced with an Illumina Novaseq 6000 to obtain paired-end 157-nt reads