GSM9724125: SCNT_2, small RNA-seq; Homo sapiens; miRNA-Seq
Total RNA including the small RNA fraction was extracted from cell pellets and assessed for integrity prior to library construction at BGI. Small RNA libraries were prepared by BGI according to their standard small-RNA sequencing workflow (project F14FTSNCHT0026_HUMrjtS, SmallRNA_Report): size-selection of the 18-30 nt fraction by denaturing PAGE, sequential 3' and 5' adapter ligation, reverse transcription with RT primer, PCR enrichment and final size selection of the ~145 bp library band on PAGE. Libraries were sequenced on an Illumina HiSeq 2000 as 49 bp single-end reads.