GSM9198492: H1417_Day7_CC90011_300nM_3; Homo sapiens; RNA-Seq
SCLC cell lines were treated, ‎for the indicated durations, with different LSD1 inhibitors. Total cellular RNA was extracted using a modified chloroform/phenol procedure (TRIzol™, Invitrogen Thermo Fisher Scientific). RNA integrity and concentration were measured using a 4200 ‎TapeStation system for high-throughput automated electrophoresis (Agilent, Santa Clara, CA, USA) RNA-Seq libraries were prepared using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) with the SPRIselect reagent (Cat. No.‎ B23318, Beckman Coulter, Indianapolis, IN, USA) following the manufacturers' protocols.