GSM9455442: HeLa KO IPPK+Pro-IP6 10 µM, Exp3; Homo sapiens; RNA-Seq
total RNA was extracted using TRIzol reagent (Cat. #15596026, Invitrogen) according to the manufacturer's instructions. Residual genomic DNA was removed with DNase I recombinant RNase-free (Cat. #04716728001, Roche), and RNA was subsequently purified using the RNA Clean & Concentrator-5 kit (Zymo Research). Library preparation was carried out using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760S/L) following the manufacturer's protocol.