GSM8818464: D458_shHSPA5_2-4_DMSO; Homo sapiens; RNA-Seq
Total RNAs were isolated using the NucleoSpin RNA, Mini kit for RNA purification (Macherey-Nagel). RNA Quality was evaluated by Bioanalyzer (Agilent Technologies) and samples with RNA integrity > 7 were used for library construction. RNA-sequencing libraries were prepared from 1µg of total RNA following the Illumina stranded mRNA prep Ligation protocol.