GSM8852328: E12TelWT; Mus musculus; RNA-Seq
Tissue was dissociated using the Neural Tissue Dissociation Kit (P) (Miltenyi Biotec) and a gentleMACS Octo Dissociator following the manufacturer's instructions. Cell clumps and debris were removed via filtration through 30 µm cell strainers (Miltenyi Biotec) followed by two consecutive rounds of centrifugation for 3 min at 200 g. Pipetting through Gel Saver tips (QSP) and a final filtration using a 10 µm cell strainer (pluriSelect) allowed to obtain a single-cell suspension. Cell viability and concentration were assessed using a MACSQuant flow cytometer (Miltenyi Biotec). Approximatively 20000 cells were used as input on the 10X Genomics Chromium controller. Chromium Single Cell 3' Library & Gel Bead Kit v3.1