GSM9668007: Vv3_48h_R2; Vitis vinifera; RNA-Seq
Total RNA was extracted according to the total RNA isolation protocol described by Reid et al. (2006), but with 10M rather than 8M LiCl, and resuspended in RNAse/DNAse-free water. The total RNA was then treated with a DNA-freeTM kit to remove any trace of DNA (Ambion – Applied Biosystems). Illumina TruSeq Stranded mRNA Sample Prep kit was used to prepare all RNA-seq libraries, following the manufacturer's instructions. Sequencing was performed by the Integragen platform. Samples were run on an Illumina HiSeq 4000 in paired mode, 2x75 bp.