GSM8986484: LGR5_CDX_derived_tumor2_scRNAseq; Mus musculus; RNA-Seq
CDX derived tumor were dissociated with gentleMACs dissociator (Miltenyi#130-093-235) and digested into single cell suspensions by using Miltenyi tumor dissociation kit (Miltenyi#130-096-730 and 130-095-929). The digested tissues were washed with PBS and filtered through a 100-µm cell strainer to obtain single cells. Droplet-based snRNA-seq libraries were prepared using the Chromium Single Cell 3' v2 according to the manufacturer's protocol