GSM9456427: C.cohnii, Glucose condition replicate 1; Crypthecodinium cohnii; RNA-Seq
Total RNA was extracted from C. cohnii using TRIzol® Reagent (Invitrogen, Life Technologies Corporation, Carlsbad, CA, USA) and purified with the RNeasy Mini Kit (QIAGEN, Hilden, Germany). RNA concentration and purity were measured with DeNovix fluorometer and Nanodrop, and integrity was assessed using Agilent 2100 Bioanalyzer. RNA-Seq libraries were prepared from 200 ng of total RNA using the QIAseq Stranded mRNA Library Kit (QIAGEN) and sequenced on an Illumina HiSeq platform (paired-end, 150 bp).