WGS of Helianthus annuus L.
For DNA isolation, plant tissues were homogenized in liquid nitrogen to a fine powder state, and 100 mg was used for DNA isolation using a CTAB-based protocol. Prior to library preparation, total DNA from sunflower was end-repaired using the NEBNext Ultra II End Repair Module (New England Biolabs, Ipswich, MA, USA). For whole-genome sequencing, libraries were prepared using the Ligation Sequencing Kit 114 (SQK-LSK114, Oxford Nanopore Technologies, Oxford, UK).