GSM9598202: scRNAseq pooled; Homo sapiens; RNA-Seq
PDX tumor tissues were mechanically dissociated and homogenized to generate single-cell suspensions. The cell suspensions were passed through a 40 µm cell strainer to remove debris and aggregates. Red blood cells were removed using red blood cell lysis buffer. The resulting single cells were washed and resuspended in cold PBS for downstream single-cell RNA sequencing. Single-cell RNA sequencing libraries were generated using the 10x Genomics Chromium platform following the manufacturer's instructions. Briefly, single cells were encapsulated into droplets using the Chromium Controller, and barcoded cDNA libraries were generated using the Chromium Single Cell 3′ Reagent Kit. Libraries were amplified and prepared according to the standard 10x Genomics protocol and sequenced on an Illumina platform.