Raw reads: BioSkryb_Cell#04-B_B2_cell_04-P33410_1016
Cultured MM.1S cells (a multiple myeloma cell-line) were incubated with propidium iodide (PI) and FACS sorted using the BD FACSAria IIu. Single or multiple cells were deposited using single cell mode into 96-well plates containing the recommended buffer for the Qiagen Repli-G MDA and BioSkryb ResolveDNA PTA kits. Cell sorting was performed on the same occasion and from the same sample of MM1.S cells to ensure minimal sample variation. For each kit, 26 wells with a single cells and two wells with 10 cells were FACS sorted into the 96-well plate. In addition, two wells with a mini-bulk of 10 cells, two positive controls wells with 1ng of genomic DNA (provided with the BioyoSkryb kit) in buffer and two empty wells (negative control containing  only with buffer) were also included in each plate. Whole Genome Amplification by MDA using the Repli-G kit (Qiagen, Cat no: 1124559, 180318 and 1124693) or PTA using the ResolveDNA kit (BioSkryb, PN: 100136, 100691 and 100940) was performed according to the manufacturerâ€™s instructions. After amplificaiton, ten random single wells, one mini-bulk and one positive control well were further selected for library preparation, using the same BioSkryb or Qiagen  kit and finally qPCR quantification prior to sequencing.