GSM8850310: H3K9me3 sample of control ESCs for dFN; Mus musculus; ChIP-Seq
Chromatins for ChIP-sequence were prepared from ESCs by following standardized method of fixation, cell lysis and fragmentation. DNA libraries for ChIP-seq were prepared according to Illumina's instructions using New England Biolab's Next ChIP-seq Library Set (#E6200) and their quantity and average size were estimated using a KAPA Quantification Kit (Millipore) and Bioanalyzer (Agilent Technology).