GSM9420662: ScRNA-seq of adjacent normal tissue; Homo sapiens; RNA-Seq
The cDNA library was prepared using the BGI MGIEasy RNA Library Preparation Kit (940-001820-00), including terminal repair, linker ligation, PCR amplification, fragment screening and purification. DNA fragments are first subjected to Rolling Circle Amplification (RCA) to form DNA Nanoballs (DNBs). Subsequently, DNBs were loaded onto the chip and Paired-End sequencing was performed using the Combinatorial Probe-Anchor Synthesis (cPAS) technique.