GSM9843868: scRNA-seq, DC BM Lin- KIThi-int cells, GEX; Mus musculus; OTHER
BM cells were harvested from three mice per genotype. Lineage-positive cells were depleted using antibodies against CD3e, CD8b, CD11b, CD19, B220, Ly-6G, and TER-119. The remaining cells were stained with fluorochrome-conjugated antibodies, labeled with unique Antibody Capture TotalSeq B antibodies (BioLegend), and sorted for lin- KIThi-int cells. Three samples from each genotype were pooled and resuspended in PBS containing 0.04% BSA at a final concentration of ~1,000 cells/uL. Single-cell suspensions were loaded onto a 10x Genomics Chromium Single Cell Controller at GTAC@MGI. Library preparation, sequencing, and Cell Ranger processing were performed by GTAC@MGI. Libraries were prepared using the 10x Genomics Chromium Next GEM Single Cell 3' Reagent Kits v3 and sequenced on an Illumina NovaSeq 6000 platform to generate 150-bp paired-end reads, targeting depths of 1 billion reads for the gene expression library and 100 million reads for the feature barcode library.