GSM8413495: sample5_4h_uninfected_DMSO; Homo sapiens; RNA-Seq
ALOs were seeded in 96-well plates at 1x105 cells per well and infected with SARS-CoV-2 (MOI=1) in the presence of 5 μM RMC-113 or 0.1% DMSO. After a 4-hour incubation, the viral inoculum was removed, and cells were washed thoroughly and supplemented with fresh medium with or without the compound. At 4 and 24 hpi, ALOs were washed and then detached via 5-min incubation with TrypLE followed by centrifuguation at 300 g for 10 minutes. Library was performed according to the manufacturers instructions (Parse Biosciences). Briefly, the pellets were suspended and fixed with Cell fixation solution (Parse, Evercode Fixation, cat: ECF2001, Part number WF303) for 10 minutes followed by incubation with Cell permeabilization Solution (Parse, Evercode Fixation, cat: ECF2001, Part number WF305) for 3 minutes at RT. ALOs were then taken out from BSL3 for single-cell sequencing according to the manufacturer's instructions (Parse, EvercodeTM WT mini Mega v2). Please find the protocol here- https://support.parsebiosciences.com/hc/en-us/article_attachments/24507636161940