GSM8887574: Hela Kyoto, Control, 1; Homo sapiens; RNA-Seq
RNA was exctracted following the protocol of RNeasy Plus Mini Kit from Qiagen The amount of total RNA was quantified using the Qubit 2.0 Fluorometric Quantitation assay (Ther-mo Fisher Scientific, Waltham, MA, USA) and the RNA integrity number (RIN) was determined using the 2100 Bioanalyzer High-Resolution Automated Electrophoresis instrument (Agilent, Santa Clara, CA, USA). RNA-seq libraries were prepared with the NEBNext® Poly(A) mRNA Magnetic Isolation Module and the NEBNext® Ultra™ II Direction-al RNA sample preparation kit (New England Biolabs, Inc., Ipswich, MA, USA). NGS library concentra-tions were quantified with the Qubit 2.0 assay and the size distribution was assessed using the 2100 Bioanalyzer instrument. Before sequencing, sample-specific NGS libraries were diluted and pooled in equimolar amounts.