Nanopore sequencing of PCR amplicons spanning the insertion junction to downstream variable rDNA regions: Replicate 3
Genomic DNA was PCR amplified using a primer specific to the 3_ end of the inserted DNA and a primer specific to a highly-conserved region ~ 2kb downstream of the R2 recognition motif in N. benthamiana rDNA. Oxford Nanopore sequencing of the purified amplicons was completed by Plasmidsaurus using R10.4.1 flowcells.