GSM9229288: CRISPR screen -UBA5-RT_high; Homo sapiens; OTHER
Genomic DNA from each bin of cells was extracted by phenocloroform. Genomic DNA from each bin was extracted and used for PCR amplification of the integrated oligos using TaKaRa Ex Taq® DNA Polymerase Hot-Start Version (Takara Bio). PCR products were purified using the MinElute PCR Purification Kit (QIAGEN) and quantified using Qubit™ fluorometric quantification (Thermo Fisher Scientific). A second PCR was performed to add Illumina indices.