Transcriptional effect of CBFA2T3::GLIS2 oncogenic fusion engineering by CRISPR-Cas9 in iPSC-derived hematopoietic cells
Transcriptional effect of CBFA2T3::GLIS2 oncogenic fusion engineering by CRISPR-Cas9 in iPSC-derived hematopoietic cells
CD41+ Flow sorted differentiation day-13 hematopoietic cells from ctrl or EG iPSC, leukemic NSG mice or patient samples were collected Cells were lysed and RNA extract using RNeasy MiniKit (Qiagen).  cDNA libraries were synthesized from 250 ng total RNA using TruSeq Strandard mRNA Kit (Illumina). Libraries were verified for their amount and quality by capillary electrophoresis using a 2100 Bioanalyzer (Agilent Technologies).