GSM8785492: Neuronal progenitor, Kabuki Syndrome 1, rep3; Homo sapiens; ChIP-Seq
ChIPseq has been performed using the True MicroChIP kit from Diagenode following the manufacturer's protocol. Briefly, cells were collected and DNA-protein was cross-linked using 36.5% formaldehyde. Then cells were lysed and chromatin was sheared by sonication (Bioruptor NextGen). Sheared chromatin was incubated with H3K4me1 Antibody (5326, Cell signalling) overnight at 4°C. Library preparation was performed by the Genomic Technologies Core facility at the University of Manchester.