Bisulfite amplicon sequencing of the 45S rDNA spacer region in Arabidopsis thaliana. Targets cytosine methylation upstream of the R2 retrotransposon nick site.
Genomic DNA was treated with bisulfite to convert cytosine residues to uracil depending on methylation status prior to PCR and amplicon sequencing to assess methylation status of rDNA upstream of the R2 insertion site.