GSM8565531: -Leucine 1 - GCN2 Inhibitor; Homo sapiens; RNA-Seq
mRNA of each sample was extracted with a NucleoSpin RNA kit (Macherey Nagel) according to the manufacturer's instructions. mRNA quality was validated by Nanodrop analysis. mRNA samples were stored at -80°C until gene expression analysis. RNA-Seq libraries were generated from 500 ng of total RNA using TruSeq Stranded mRNA Library Prep Kit and TruSeq RNA Single Indexes kits A and B (Illumina, San Diego, CA), according to manufacturer's instructions.