WGS of Mycobacterium tuberculosis H37Rv: delta nucS
H37Rv delta nucS genomic DNA was extracted following the standard protocol for preparation of high-quality mycobacterial genomic DNA (Belisle, J. T., Mahaffey, S. B. & Hill, P. J. Isolation of mycobacterium species genomic DNA. Methods Mol Biol 465, 112 (2009).). The integrity of each DNA sample and the absence of RNA contamination were confirmed by DNA agarose gel electrophoresis, while its concentration and purity were measured using a NanoDrop-2000 spectrophotometer (Thermo Fisher Scientific). Genomes were sequenced through Illumina MiSeq sequencing using a MiSeq v.2 sequencing kit to obtain 250-bp paired-end reads (StabVida)