GSM8888250: 1.5hpi, ATRX, rep1; Homo sapiens; Human alphaherpesvirus 1; ChIP-Seq
Cells werecrosslinked with 2mM ethylene glycol-bis(succinimidylsuccinate) (EGS)for 45 min at room temperature, followed by a 1% formaldehyde fixation for 8 min. After quenching and lysis, chromatin was sonicated using a Bioruptor Plus (Diagenode) (45 cycles of 30s on/30s off, 4C) NEBNext Ultra II DNA Library Prep Kit for Illumina (#E7645S, NEB), without size selection