GSM8711411: MiaPaca2 DMSO-1 RF; Homo sapiens; OTHER
Total RNA and ribosome-protected fragments were isolated following published protocol (Ingolia, N.T.; Brar, G.A.; Rouskin, S.; McGeachy, A.M.; Weissman, J.S. The ribosome profiling strategy for monitoring translation in vivo by deep sequencing of ribosome-protected mRNA fragments. Nat. Protoc. 2012, 7, 1534–1550. https://doi.org/10.1038/nprot.2012.086) Deep sequencing libraries were generated from these fragments and sequenced on the HiSeq 2000 platform. Genome annotation was conducted with the human genome sequence GRCh38 downloaded from the Ensembl public database (http://www.ensembl.org)