GSM9724310: RNA-seq of heart_4; Mus musculus; RNA-Seq
Total RNA was extracted from heart tissue using TRIzol reagent (Invitrogen) followed by RNeasy Mini Kit (Qiagen) purification with on-column DNase digestion. RNA integrity was assessed by Agilent 4200 TapeStation (RIN ≥ 7.0). Libraries were prepared using NEBNext Ultra II RNA Library Prep Kit (NEB) per manufacturer's instructions. AMPure XP beads were used for size selection (300–400 bp).