GSM9456968: control organoids, T5224 treated, 24 h, rep1; Mus musculus; ATAC-seq
Bulk ATAC-seq was performed as described in SHARE-seq (Ma et al. 2020) using Tagify Tn5 enzyme from SeqWell. Genomic DNA was extracted following ATAC-seq using a Qiagen MinElute Kit. Purified DNA was PCR amplified using NEBNext 2X MasterMix with a unique i5 and i7 index pairing per sample. Final libraries were purified using Zymo DNA Clean & Concetrator Kit.