GSM8710015: Line XIII H3K4me3 ChIP-seq rep 1; Homo sapiens; ChIP-Seq
Briefly, cells were crosslinked with 1% formaldehyde, lysed, and sonicated using a Bioruptor Pico (Diagenode) to generate chromatin fragments <500bp. For each ChIP, 20-30ug of sonicated chromatin was used and immunoprecipitated on magnetic Dynabeads (Pierce #88802). The following antibodies were used: IgG (Santa Cruz, #sc-2027), mouse anti-H3 (Upstate/EMD-Millipore, #05-499), rabbit anti-H3K4me3 (Millipore 04-745). Libaries were prepared using the NEB Next Ultra II DNA Library Prep Kit