GSM8676444: K562, Non-targeting sgRNA, rep2; Homo sapiens; ATAC-seq
DNA was purified with the Qiagen MinElute PCR Purification Kit Cells were washed twice with PBS and resuspended in PBS. 10, 000 cells in 5 µL PBS were mixed 42.5 µL of transposition buffer. The reaction was incubated at room temperature for 10 minutes, followed by the addition of 2.5 µL of assembled Tn5 transposase, and then incubated at 37 °C for 30 minutes in a Thermomixer set to 300 rpm. DNA was subsequently purified with the Qiagen MinElute PCR Purification Kit and minimally amplified for sequencing. Final libraries were purified again with the MinElute PCR Purification Kit.