AMPLICON of Severe acute respiratory syndrome coronavirus 2: Human NJ-BioR-589-Ampliseq
The NJ-BioR-589-Ampliseq library was prepared using the AmpliSeq Library PLUS and cDNA Synthesis for Illumina, with 20ng input, amplified with the AmpliSeq for Illumina SARS-CoV-2 Research Panel, ligated with AmpliSeq CD Indexes for Illumina, enriched with 18 cycles of PCR, and sequenced on Illumina NovaSeq 6000 with 2x150bp reads targeting 0.5M read coverage.