GSM9724102: SCNT_2_H3K27ac, H3K27ac ChIP-seq; Homo sapiens; ChIP-Seq
Cells were cross-linked with 1% formaldehyde for 10 min at room temperature, quenched with 125 mM glycine, lysed and sonicated to 200-500 bp on a Covaris-class focused ultrasonicator. Chromatin was immunoprecipitated with anti-H3K27ac (Abcam ab4729). A 1% aliquot of the same lysate (no IP) was kept as matched whole-cell extract input. Eluted ChIP and input DNA were end-repaired, A-tailed and ligated to indexed Illumina adaptors using a standard ChIP library workflow, then PCR-amplified and size-selected to 200-450 bp. Libraries were sequenced on an Illumina NextSeq 500 (instrument NB501674) as 76 bp single-end reads.