Metagenome Sequencing of LA3
DNA was extracted using FastDNA SPIN Kit for Soil (MP Biomedicals). DNA quality and concentration were assessed using Qubit 4 Fluorometer and Nanodrop. The 16S rRNA gene (V1V9 region) was amplified using primers 27F and 1492R with LongAmp Taq 2X Master Mix (NEB). Amplicons were purified using AMPure XP beads and quantified. Libraries were prepared using Oxford Nanopore 16S Barcoding Kit (SQK-16S114.24) and sequenced on PromethION platform using R10.4.1 flow cells.