GSM9698979: SH1000 pEPSA5::sroA 3hr/3hr; Staphylococcus aureus; RNA-Seq
Total bacterial RNA was isolated utilizing the Qiagen RNeasy mini kit and contaminating DNA was removed by treatment with Ambion DNA-free kit. Total RNA was enriched for mRNA using MicrobExpress kit and Illumina RiboZero rRNA removal kit for Gram-positive bacteria. Library construction was performed using the TruSeq Stranded mRNA Kit from Illumina omitting mRNA enrichment steps.