GSM8677680: PBS 3; Mus musculus; RNA-Seq
Mice were sacrificed and the tongue was excised and injected at several sites along the tongue with 0.5 mL of 4mg/ml Dispase in PBS + 2% FCS until fully distended. After incubation in PBS+ 2% FCS for 10 min at 37°C the epithelium was carefully separated using forceps and then rinsed with PBS. Epithelial tissues were then minced and treated with a Collagenase type II (2 mg/mL; Worthington Biochemicals) and DNase I (1 mg/mL; Sigma) solution in PBS plus 2% FCS for 25 min at 37 °C in a shaker bath. A total of 20 μL of 0.5 M EDTA per 2 mL sample was added to the digested tissues and incubated for an additional 10 min. The cells were washed, filtered with 70-μM filter. mRNA kapa stranded kit done by Abed