Pacbio_Library_1_hifi_reads
Targeted amplification of integrated lentiviral vectors from human genomic DNA using a single-end UMI workflow (LUNa-seq). In round 1, PCR generates UMI-tagged on-target molecules. Round 2 performs semi-nested PCR to specifically enrich targets. Round 3 applies nested PCR to further increase specificity and append sample-specific barcodes. The expected amplicon size is ~310 kb. Data are submitted as basecalled reads (FASTQ).