GSM8853210: s_no_comp_1__Uncomp; Homo sapiens; RNA-Seq
RNA was extracted from cells using the RNeasy mini kit (Qiagen, #74104) All samples underwent RiboGreen quantification Agilent BioAnalyzer quality control. 500 ng RNA of each sample were submited for polyA selection and TruSeq library preparation using 8 PCR cycles according to Illumina instructions (TruSeq Stranded mRNA LT Kit, #RS-122-2102). After barcoding samples were run on a HiSeq 2500 in a 50 bp/50 bp paired-end run using the HiSeq SBS Kit v4 (Illumina).