GSM9045516: Detroit562_H3K36M_OE_RNAseq_rep1; Homo sapiens; RNA-Seq
Total RNA was extracted from approximately 1 million cells using the AllPrep DNA/RNA/miRNA Universal Kit (QIAGEN) and following the manufacturer's protocol, including DNase treatment option. Library preparation was performed using the NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat) (New England Biolabs) following the manufacturer's protocol (including RNase H and DNase I digestions). 100-bp paired-end sequencing was performed on the Illumina HiSeq 4000.