GSM9198454: MCF-7/ADR cells; Homo sapiens; RNA-Seq
RNA was extracted using TRIzol (Invitrogen) Sequencing libraries were prepared from rRNA-depleted RNA using the VAHTS Universal V8 RNA-seq Kit for Illumina (Vazyme, NR611)per manufacturer instructions. RNA was fragmented (~300 bp) and reverse transcribed into cDNA. During second-strand synthesis, dTTP was replaced with dUTP to enable strand-specificity. Following adapter ligation, the **dUTP-marked second strand was digested with USER enzyme, preserving only the first strand. Libraries were then PCR-amplified, indexed, and purified.Final libraries were pooled and sequenced on an Illumina NovaSeq 6000 platform for PE150 sequencing.