Cherry_Radish_T4
RNA samples were qualified (1 g input). Libraries were prepared with NEBNext Ultra RNA Library Prep Kit for Illumina (NEB). mRNA was selected by poly-dT beads, fragmented, and double-stranded cDNA synthesized. After end repair, A-tailing, and adaptor ligation (NEBNext), fragments of ~240 bp were selected with AMPure XP beads. Following USER enzyme treatment, libraries were amplified with index primers and assessed on an Agilent Bioanalyzer 2100.