GSM9096884: ATACseq OCI-AML3 H1.3 KO clone 1; Homo sapiens; ATAC-seq
ATAC-Seq were performed with the Diagenode ATAC-seq kit; (#C01080001) following the manufacturer's instructions. Nuclei were collected by centrifugation, and a transposition reaction was performed by adding 50 μL of transposition mixture (containing Tagmentation buffer, Tagmentase, 2% Digitonin, 10% Tween20, PBS and Nuclease-free water) for 30 min at 37°C. DNA was isolated using supplied spin columns and eluted in 12 μL of DNA elution buffer. Transposed DNA fragments were amplified using 8 UDI for Tagmented libraries (Diagenode; #C01011035) and libraries were purified using AMPure XP beads (Beckman Coulter; #A63881) Libraries were prepared using the ATAC-seq kit (Diagenode) with 24 unique dual indexes (UDI) for tagmented libraries (Set I), according to the manufacturer's protocol, and analyzed on an Agilent 2100 Bioanalyzer prior to sequencing.