WuLi
Highmolecularweight genomic DNA ( 50 kb) was extracted and 1.5 g was used for library preparation with the Oxford Nanopore Technologies (ONT) Ligation Sequencing Kit (SQKLSK114). After end repair and adapter ligation, the library was loaded onto a R10.4.1 flow cell (FLOMIN114) and sequenced on a PromethION 2 Solo device for 72 hours. Realtime basecalling was performed with Guppy v6.4.2 in highaccuracy mode, and reads with Qscore < 7 or length < 200 bp were filtered out.