GSM9844027: Cornea Pitx2 -/- male rep3; Mus musculus; RNA-Seq
Frozen corneas were shipped on dry ice to Beijing Genomics Institute (BGI, Shenzhen, China) for library construction and sequencing on the DNBseq platform. Total RNA was extracted using the RNeasy Mini Kit (Qiagen). Polyadenylated RNA was captured with oligo(dT)-coupled magnetic beads and fragmented. RNA integrity and fragment-size distributions were assessed on a Fragment Analyzer; only samples meeting predefined QC thresholds advanced to library preparation. Libraries were generated with the BGI Optimal Dual-mode mRNA Library Prep Kit and sequenced on a DNBSEQ-T7 (paired-end 2×100 bp; ~50 million clean reads per sample). Adapters, contaminants, and low-quality bases/reads were removed with SOAPnuke (Li et al., 2008).