GSM8566378: 25 pg/mL TNF S7; Homo sapiens; RNA-Seq
Co-cultures were  untreated (0 pg/ml) or treated with TNF (25 pg/mL, 10 ng/mL, and 100 ng/mL ) for 7 days, and cell were lysed using RLT buffer containing β-mercaptoethanol, and the supernatant collected. Total RNA was extracted and purified from the collected supernatant using the RNAeasy mini spin columns (Qiagen). Poly(A)+-enriched cDNA libraries were generated using the Illumina TruSeq RNA Library Prep kit v2 (Illumina Inc., Hayward, CA, USA).