GSM9018365: Sample 9, soleus muscle, space; Mus musculus; RNA-Seq
Bead beater device (BioSpec) was used to homogenate the soleus muscle in TriZol (Invitrogen), followed by chloroform gradient separation and ethanol dilution.  Direct-zol RNA Miniprep Kit (Zymo) was used to purify the RNA. rRNA-depleted libraries were prepared from 90 ng of total RNA using Ribozero kit (Illumina), and sequenced in pair-end sequenced mode on the NovaSeqXPlus platform (Illumina).