Fimbristylis miliaceae Chloroplast genome: 3-leaf stage
Fresh leaves and stems were harvested from Fimbristylis miliacea collected from cherry blossom nursery in Shanghai (N 30.99, E 121.04), China, in 2022. Total genomic DNA was extracted using a modified cetyltrimethylammonium bromide method and applied to a 500-bp paired-end library construction using the NEBNext Ultra DNA Li-brary Prep Kit (NEB, USA) for Illumina sequencing. Sequencing was performed on an Illumina NovaSeq 6000 platform (BIOZERON Co., Ltd, Shanghai, China). De novo as-sembly with NOVOPlasty, and referencing the cp genome of closely related species, produced two options of circular contigs of the cp genome. The contig with the higher homology cpDNA was selected as the candidate cp genome.