GSM9036535: p.Tyr622* + CDP-choline (last 14 days)) replicate 2; Homo sapiens; RNA-Seq
Total RNA was extracted from induced neurons using the RNeasy Mini Kit (Qiagen), and RNA quality was assessed using a 5300 Fragment Analyzer (Agilent). Only samples with an RNA Quality Number (RQN) greater than 9.5 were selected for library preparation. Full-length cDNA was synthesized using the SMART-seq v4 kit (Takara Bio), and sequencing-ready libraries were subsequently prepared using the Nextera XT DNA Library Preparation Kit (Illumina). Libraries were sequenced as 75 bp + 75 bp paired-end reads with dual 8-nucleotide indexes on an Element AVITI sequencing platform (Element Biosciences) at the MIT BioMicro Center.