WGS of Escherichia coli BW25113: isolates from population co-evolved under medium osmolality
Whole-genome sequening was performed on bacterial isolates from a bacteria-phage co-evolution experiment in different osmotic conditions. Isolates were obtained by streaking frozen glycerol stocks onto LB-agar plates, picking single colonies, and restreaking twice more. DNA was extracted from liquid cultures grown from single colonies using the DNeasy Blood and Tissue Kit (Qiagen) according to the manufacturer's instructions. Library preparation and sequencing were performed by Plasmidsaurus. Genomic libraries were prepared using the Illumina DNA Prep kit and sequenced with the Illumina NovaSeq platform generating paired end 150 bp reads.