Oxford Nanopore sequencing data of Rhodiola yunnanensis
For Oxford Nanopore sequencing, high-quality genomic DNA was extracted using a modified CTAB method and used for library preparation with the SQK-LSK114 ligation sequencing kit. High-molecular-weight DNA fragments were size-selected using magnetic beads, followed by damage repair, end repair, and adapter ligation. The final libraries were quantified using a Qubit fluorometer, loaded onto R10.4.1 flow cells, and sequenced on the PromethION platform (Oxford Nanopore Technologies, Oxford, UK).