GSM9556868: NZBW_Kidney_10w_rep3; Mus musculus; RNA-Seq
Tri Reagent (Sigma-Aldrich) was used followed by column-based purification with RNA Clean & Concentrator-5 Kit (Zymo Research). NEBNext Poly(A) mRNA Magnetic Isolation kit (New England BioLabs) was used to generate cDNA libraries. Picogreen dsDNA quantitation assay (Thermo Fisher Scientific) was used to measure the cDNA concentration; cDNA was diluted to 3 nM, cDNA libraries were pooled together