GSM9199037: sgERG HiChIP; Homo sapiens; OTHER
After in-situ proximity ligation, samples were sonicated on Covaris LE 220 at 4C, 300W, 15% duty factor, 200 cycles per burst, for 300 seconds. Samples are then precleared with protein G beads for an hour and subsequently incubated with 5μg of H3K27AC antibody (ab4729, Lot:GR3231988) at 4C overnight. After IP, libraries were amplified and quantified using KAPA Library Amplification kit HiChIP libraries were generated using the Arima-HiC+ kit (A410232) with Swift Biosciences® Accel-NGS® 2S Plus DNA Library Kit (Cat # 21024) and Indexing kit (Cat # 26148) according to manufacturer's protocol with minor modifications