GSM9198851: Differentiated SH-SY5Y cells treated with Morphine, replicate 1; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol reagent (Invitrogen) according to the manufacturer's protocol, followed by DNase I treatment to remove genomic DNA contamination. RNA quality and integrity were assessed using Bioanalyzer (Agilent). RNA-seq libraries were prepared using the Novogene NGS RNA Library Prep Set (PT042) for 250–300 bp insert cDNA libraries, without strand-specific information. Sequencing was performed on the Illumina NovaSeq 6000 platform, generating paired-end 150 bp reads.