GSM9128573: RNA-seq of rat spinal cord (pooled); Rattus norvegicus; RNA-Seq
Rat spinal cords were stored and shipped in sCelLive® Tissue Preservation Solution (Singleron Biotechnologies GmbH). RNA was isolated with the GeneMAtrix Universal RNA Purification Kit (Roboklon) and subsequently processed according to the instructions of the AccuraCode® RNA-Seq kit (Singleron Biotechnologies GmbH) Total RNA was extracted using the GeneMAtrix Universal RNA Purification Kit (Roboklon). mRNA was captured with AccuraCode® bulk beads (Singleron Biotechnologies GmbH) and reverse transcribed into cDNA with sample-specific barcodes and unique molecular identifiers (UMIs). Libraries were constructed with the AccuraCode® RNA-Seq Kit (Singleron Biotechnologies GmbH) and sequenced on an Illumina NovaSeq 6000 (paired-end 150 bp).