Shotgun sequencing of Canis lupus familiaris
Genomic DNA was extracted from fecal samples using the Mo-Bio PowerSoil Kit, and DNA quality was assessed via gel electrophoresis with concentrations measured using a Qubit 3.0 Fluorometer. Library preparation and shotgun metagenomic sequencing were performed, with paired-end sequencing conducted on an Illumina NovaSeq S4 platform.