RNA seq of Spirodela polyrhiza
The total RNA was isolated using ReliaPrep RNA Tissue Miniprep System (Promega, USA). RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-8000 (Nanodrop Technologies), and RNA integrity were assessed using a BioAnalyzer Nano kit (Agilent). Subsequently, RNA-seq libraries were prepared using the Illumina Stranded mRNA Sample Prep Kit and sequenced on the Illumina NextSeq 500 platform with pairedend 150 bp at the VIB Nucleomics Core