GSM9199100: Tumor, Untreated, negative control; Mus musculus; RNA-Seq
The cell suspension was prepared according to general protocols and loaded into Chromium microfluidic chips with 3' (v3.1) chemistry and barcoded with a 10X Chromium Controller (10X Genomics). RNA from the barcoded cells was subsequently reverse-transcribed. The sequencing libraries were constructed using a Chromium Single Cell 3' (v3.1) reagent kit (10X Genomics) (Novogene). Illumina NovaSeq 6000 PE150 was used for sequencing following the manufacturer's instructions.