GSM8819206: KT078_PM_positive_cfDNA_5hmC; Homo sapiens; OTHER
On average 9.7ng (IQR: 9.7-10.0) was obtained from ~5mL of peripheral blood collected from each patient using QIAamp Circulating Nucleic Acid Kit (Qiagen, Cat. No. 55114). 5hmC libraries for all samples were constructed with high-efficiency hmC-Seal technology. According to the requirements of next-generation sequencing, the cfDNA extracted from plasma was end-repaired, 3′-adenylated using the KAPA Hyper Prep Kit (KAPA Biosystems), and then ligated with the Illumina compatible adapters.