GSM9668747: 4T1_control_0h_biol rep1; Mus musculus; RNA-Seq
4T1 cells were treated with or without TGFβ1 for 4 days, then trypsinzed and the cell pellets were lysed in Zymo 1X DNA/RNA Shield™ (Zymo Research #R1100-50) RNA isolation, library construction and sequencing was done by Plasmidosaurus (Briefly, mRNA is converted into cDNA via reverse transcription using a poly(dT)VN primer, followed by second-strand synthesis, and then tagmentation, library indexing, and amplification. This approach uses 3' end counting to capture differential gene expression. The single end sequencing is stranded and sequences towards the 3' end. Unique molecular identifiers (UMIs) are used to deduplicate, and unique dual indices (UDIs) to prevent index hopping). All experiments were performed in three biological replicates.