GSM8567256: lib94754; Mus musculus; RNA-Seq
Cells were harvested at 0 and 5 hours and RNA isolated using Qiagen RNeasy kits (Qiagen). RNA was used to construct libraries using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara), with reverse transcription followed by polymerase chain reaction amplification to generate full length amplified cDNA. Sequencing libraries were constructed using the NexteraXT DNA sample preparation kit with unique dual indexes (Illumina) to generate Illumina-compatible barcoded libraries. Libraries were pooled and quantified using a Qubit Fluorometer (Life Technologies).