RNA-Seq of Thalassiosira pseudonana: vegetative cells biological replicate2
mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. Following fragmentation, cDNA was synthesized using random hexamer primers, and the library was constructed through end repair, Atailing, adapter ligation, size selection, amplification, and purification. After Illumina sequencing and data quality control, clean reads were mapped to the reference genome of T. pseudonana (ASM14940v2).