GSM8989066: Multiome_snATAC-seq, HGSOC_Unenriched_Tumor; Homo sapiens; ATAC-seq
The fresh tissues were washed with cold PBS and first mechanically disrupted to generate small pieces. Then, they were subjected to enzymatic digestion with the Tissue Dissociation Kit (Miltenyi Biotec, 130-095-929) following the manufacturer's instructions. Enzymatic digestion was carried out for 2 hours at 37° C with shaking at 350 rpm. The digested samples were then filtered through 70 micron cell strainers and subjected to the red-blood cell lysis using RBC lysis buffer (eBioScience, 00-4333-57). The isolated nuclei were immediately used for transposition, followed by GEM generation and barcoding, cleanup and library pre-amplification PCR following the Chromium Next GEM Single Cell Multiome ATAC + Gene Expression user guide (10X Genomics, Rev E). The pre-amplified samples were used to generate ATAC library construction and cDNA amplification for Gene Expression library construction.