HASMC RNA-seq
The total RNA of the vascular smooth muscle cells was isolated using TRIzol (Invitrogen, USA) and used to generate a cDNA library. Then, RNA sequencing was conducted using the MGISEQ 2000 platform (BGI-Shenzhen, China). RNAs differential expression analysis was performed by differentially expressed genes sequence (DEGseq). Genes with adjusted P-value < 0.05 and |log2 (fold change) |  1 were deemed to be differentially expressed. KEGG-DOT plot and GO-Dot plot were generated with the BGI Dr. Tom system (https://www.biosys.bgi.com)