Amplicons for soil microbes under ambient and drought treatment
The DroughtDiversity Manipulation Experiment (DIGME) is a multi-site field study investigating microbial responses to altered precipitation across diverse ecosystem types, including drought and ambient treatments. Soil DNA was extracted from ~0.25 g of soil using the DNeasy PowerSoil Pro Kit (Qiagen). Fungal communities were characterized by amplifying the ITS1 region using ITS1F and ITS2 primers.