GSM8386035: PA3215-2; Pseudomonas aeruginosa PAO1; ChIP-Seq
P. aeruginosa  was cultured in LB medium until mid-log phase (OD600 = 0.6), then treated with 1% formaldehyde for 10 min at 37℃. After quenching and lysis, cells were subjected to sonication to produce 100–300 bp DNA fragments. ChIP-seq libraries were constructed using NEXTflex ChIP-Seq kit (Bio Scientific), and agarose gel was used to cut DNA fragments between 150 and 250 bp.