GSM9844628: BDII_1; Homo sapiens; ATAC-seq
Forebrain organoids were washed three times with PBS. Nuclei were extracted from the samples and resuspended in Tn5 transposase reaction mix. The transposition reaction was incubated at 37°C for 30 min. ATAC-seq libraries were prepared using a Tn5 transposase-based protocol. After transposition, equimolar Adapter 1 and Adapter 2 were added, and PCR amplification was performed to generate sequencing libraries. The amplified libraries were purified using AMPure beads, and library quality was assessed using Qubit. Index-coded libraries were clustered on a cBot Cluster Generation System using the TruSeq PE Cluster Kit v3-cBot-HS according to the manufacturer's instructions. After cluster generation, libraries were sequenced on an Illumina HiSeq platform to generate 150 bp paired-end reads.