ITS metabarcoding analysis of soil: barcode 66
Samples consisted of cores of topsoil (0-10 cm), sampled with a metal corer ( 1 cm). DNA was extracted following dx.doi.org/10.17504/protocols.io.4r3l29b9jv1y. Soil DNA was used to amplify the ITS ribosomal RNA gene, using primer pair ITS9munngs -ITS4ngs followed by Oxford Nanopore MinION Mk1C sequencing.