Tunneling nanotubes mediate mitochondrial homeostasis in cancer
Tunneling nanotubes mediate mitochondrial homeostasis in cancer
GSC (+) have received astrocytic mitochondria (mito-dsRED+) and without astrocytic mitochondria (mito-dsRED-) were sorted after 4 days of co-culture in 2D-culture with murine AS expressing mito-dsRED in a ratio of 3 D to 1 Acc (GSC +), to allow the accumulation of mitochondria, from three independent experiments.  For the extraction, we use the RNeasy microkit Qiagen cDNA libraries were prepared using Illumina Stranded mRNA library Preparation Kit (Illumina) following the manufacturerâ€™s protocol from 250 ng of RNA. Pooled libraries were additionally purified of unbound adaptors and primer dimers on AMPure XP magnetic beads (Beckman-Coulter).