RNA-Seq of maize (Zea mays) seedlings
This study employs RNA-Seq technology to conduct an in-depth analysis of the transcriptome under specific cellular conditions. It undergoes meticulous steps including RNA extraction, purification, cDNA synthesis, library construction, amplification, and purification to ensure the quality and accuracy of the library and quantification. Utilizing advanced instruments such as the Hieff NGS kit, Qubit 4.0 fluorometer, and Nano-300 spectrophotometer, the study guarantees the construction and precise quantification of the library. The STAR software is used for aligning sequencing data, while DESeq2 software is employed for the analysis of differentially expressed genes, unraveling the mechanisms of gene expression regulation.