GSM9370478: AtT20, p300 overexpression treated with BRC, IgG; Mus musculus; OTHER
Cell samples were washed twice with wash buffer containing 20 mM HEPES (pH 7.5), 150 mM NaCl, 0.5 mM Spermidine, and protease inhibitors (cat. no. G2007, Servicebio). For tissue samples, cell nuclei were isolated using lysis buffer containing 0.1% Triton X-100. 10 μL of Concanavalin A-coated magnetic beads (cat. no. 14203D, Thermo Fisher) were added to each sample and incubated at room temperature for 10 min. Bead-bound cells were immunoprecipitated with specific antibodies, followed by secondary antibodies to enhance binding sites. After washing, cells were treated with PAG-Tn5 transposase complex to obtain DNA fragments, which were amplified by 15 cycles of PCR to construct libraries. Libraries were sequenced on the Illumina NovaSeq Xplus platform (cat. no. FC-410-1001, Illumina) with paired-end 150 bp reads