GSM9155565: SPG21_siRNA_4; Homo sapiens; RNA-Seq
RNA was isolated using Qiagen RNeasy Mini Kit. 5 μl for axon samples and 1 μl for neuron samples were used for synthesizing cDNAs using a SMART-Seq version 4 Ultra Low Input RNA Kit for Sequencing (Takara). A Nextera XT DNA Library Preparation Kit (Illumina) was used to prepare libraries from the cDNA samples using 1 ng of cDNA.