GSM9744492: GPA-F-74-P023; Homo sapiens; RNA-Seq
Cryopreserved PBMCs were thawed by adding 10 mL warm RPMI containing 10% FCS. Next, samples were washed twice with warm RPMI+10% FCS to remove DMSO. Single-cell mRNA sequencing was performed at Single Cell Discoveries according to standard 10x Genomics 3' V3.1 chemistry protocol. Cryopreserved cells were thawed and counted to assess cell integrity and concentration prior to loading on the 10X Genomics controller. The resulting sequencing libraries were prepared following a standard 10x Genomics protocol.