GSM9287400: 20433X1 WT Vehicle C1498-OVA AML; Mus musculus; RNA-Seq
WT and miR-155 TCKO littermate mice each received mRNA-OVA LNP or vehicle control after challenge with an OVA-expressing C1498 AML cell line, in two doses at Day 1 and Day 7 of the experimental procedure. For each dose, 2.5 µg of mRNA vaccine was mixed with 10 µl RNAiMAX Transfection Reagent (Thermo Fisher), assembled at room temperature for 15 min after delicate mixing, and diluted to working concentration using 85 µl of PBS under sterile conditions. Each dose was then injected as 100 µl total mRNA-OVA-LNP solution, or the same solution without RNA as the vehicle control. At Day 21, all mice were injected with 2.5 million cells per mouse of the C1498-OVA AML line via tail vein. 10x Genomics Chromium Next GEM Single Cell  5' v2 Gene Expression Library Preparation