GSM9197696: TRE3G 2xFLAG-GSX2 WT Day 10.5, 2% Input, Replicate 2; Homo sapiens; ChIP-Seq
Monolayers were dissociated with Accutase for 30 minutes and fixed in 1% formaldehyde in DPBS for 10 minutes at room temperature. After quenching and cell lysis, chromatin was sonicated using a Covaris S220 sonicatior Libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina and Multiplex Oligos for Illumina kits (NEB). 15µg of sonicated chromatin was used as starting material for input and IP. 60ng of each reaction  were amplified for 4-5 cycles and then size-selected for <500bp. Agilent high-sensitivity D1000 screentapes were used for library QC prior to sequencing.