ONT sequencing of the Zi goose
This document describes the "1D Genomics DNA by Ligation" method involving three main steps: DNA fragmentation (optional for low input, using Covaris g-TUBE for physical breaking and analyzing fragment quality), DNA repair and end-preparation (mixing with repair reagents like NEBNext FFPE DNA Repair Mix, incubating at 20C and 65C, and purifying via AMPure XP beads), and adapter ligation and clean-up (adding AMX and LNB, incubating at room temperature, purifying with magnetic beads, selecting LFB for 3 kb or SFB for <3 kb fragments, and eluting to recover the final DNA library). Core reagents include ligation buffer, adapter mix, and fragment buffers, suitable for genomic DNA library construction.