small RNA-Seq of Homo Sapiens: adult plasma
Plasma miRNAs were isolated using the Plasma/Serum Circulating and Exosomal RNA Purification Kit (Norgen Biotek, Canada) and sequenced using a previously validated protocol (PMCID: PMC6557167). Small RNA libraries were constructed using the NEBNext Small RNA Library Prep Set (New England Biolabs, Ipswich, MA, USA) with 1:6 adapter dilution ratio and 17 cycles of PCR amplification. The libraries were cleaned, quality-checked, and sequenced on a HiSeq 4000 with 75bp single end mode.