GSM8567458: WT_NR3; Mus musculus; RNA-Seq
Hippocampus tissue from Thy1-hTau.P301S mice was resuspended in Trizol and dispersed by shaking. Aliquots were separated by agarose gel electrophoresis to ascertain RNA integrity and purity. NanoPhotometer spectrophotometry and the Agligent 2100 bioanalyzer were also used to confirm purity and integrity of RNA samples, respectively. RNA libraries for RNA-seq were prepared using Illumina's NEBNext® UltraTM RNA Library Prep Kit following manufacturer's protocols