Illumina HiSeq 2500 sequencing of SAMD00855813
10 to 15 micrograms of total RNA was separated by electrophoresis in 24% denaturing polyacrylamide gels containing 4 M urea, and stained with SYBRGold (Invitrogen). Small RNA fraction with length of around 20 to 30 nt were recovered using the ZR small-RNA PAGE Recovery Kit (ZYMO Research). Library construction of small RNA was performed by using TruSeq RNA Sample Prep Kits (Illumina) according to manufacturer's protocols.