GSM9042417: mESC, WT, Wdr5 ChIP-seq, rep1; Mus musculus; ChIP-Seq
Chromatin fragmentation by Mnase and immunoprecipitation were carried out following the SimpleChIP Enzymatic Chromatin IP Kit (Cell Signaling Technology) according to the manufacturer's protocols. Purified ChIP DNA was used for library construction following the Illumina ChIP–seq library generation protocol (NEB)