GSM9724298: RNA-seq of heart_1; Mus musculus; RNA-Seq
Total RNA was extracted from left ventricular myocardial tissues (including the infarct core and peri-infarct regions) using TRIzol reagent (Invitrogen). RNA quality was assessed using an Agilent 2200 system, and samples with RNA integrity number (RIN) > 7.0 were used for library preparation. RNA-seq libraries were constructed using the VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina (Vazyme) following the manufacturer's instructions. Library quality was validated using the Agilent 2200 system. Sequencing was performed on the DNBSEQ-T7 platform (150 bp paired-end) by NovelBio.