RNA_Chr4+5_haESC-3 (CRX2018158; CRA030371)
RNA was treated by NEBNext UltraTM RNA Library Prep Kit for Illumina. In brief, first strand cDNA was obtained by PCR of mRNA enriched from total RNA using poly-T-oligo-attached magnetic beads. Then cDNA was purified, end-repaired, followed by A-tailing and adapter-adding. The libraries were enriched using PCR, and sequenced on the Illumina platform and 150-bp reads were obtained