GSM8851799: scRNA_seq-A8_DHA1_6h-1; Plasmodium falciparum; RNA-Seq
Highly synchronized parasites were subjected to Hoechst 33342 staining for 10 minutes at 37℃, followed by sorting of positive stained cells into 0.05% BSA/PBS solution using a FACSAria II sorter (BD Biosciences). The sorted cells underwent two washes with 0.05% BSA/PBS， were re-suspended in 100 μL 0.05% BSA/PBS, and counted using a cell counting chamber. libraries were prepared using the Chromium Single Cell 3ʹReagent Kits v3 (10x genomics) following the instructions provided in the user guide.