GSM8988016: Venus positive, mCherry negative cells Wildtype replicate 1; Danio rerio; RNA-Seq
At 48 hpf, heads of TgBAC(apln:Venus-PEST); Tg(kdrl:Hsa.HRAS-mCherry) larvae were disscected. After dissection, the heads were washed in Ca2+/Mg2+-free Hank's Balanced Salt Solution (HBSS, Gibco, 14175-053) and cell dissociation was performed at 28,5°C for 30 min with TrypLE Express (Gibco, 12604-013).  Cell dissociation was stopped by FBS addition and (Gibco, 12604-013) and cells were centrifuged for 3 min at 3000 rpm. HBSS was removed and the cell pellet was resuspended in ice-cold HBSS supplemented with 5% FBS and filtered through 40 µm filter caps. Afterwards, the cells were centrifuged again for 3 min at 3000 rpm and resuspended in HBSS + 5% FBS. Library construction was performed by BGI Genomics.