RNA-Seq of homo sapiens: NK cells
One month after isolation, modified NK cells were harvested in RLT buffer. Surviving AnnexinV-PE- SYTOX-AADvanced- GFP+ (CID)-iCasp9-NK cells were sorted. Total RNA was extracted from the frozen samples with TRIzol (Invitrogen, Carlsbad, USA). RNAseq libraries were prepared with KAPA RNA HyperPrep Kit and KAPA UDI Primer Mixes (Roche) and sequenced on Illumina NovaSeq X (pairedend).