GSM9197037: RFP; Mus musculus; Bisulfite-Seq
Genomic DNA was extracted from each sorted population (200,000 cells per condition), which served as input for bisulfite conversion, carried out using the EpiJET Bisulfite Conversion Kit (Thermo K1461), following the manufacturer's instructions. Sox2 SCR region containing 7xCpG was amplified by PCR. Amplified products were gel-purified and submitted for sequencing with MiSeq Nano.