Spatial transcriptomics of tea leaf bud (CRX1983410; CRA030155)
Same steps as tissue optimization, tissue sections were affixed onto gene expression slides for fixation, H&E staining, and bright field imaging. The cDNA was then synthesized from the captured mRNA and prepared into a sequencing library following previously described. Supernatants containing the released cDNA were collected and transferred into 96-well plates, and ST sequencing libraries were prepared using an automated MBS 8000 system. Paired-end sequencing was performed on an Illumina NovaSeq platform (Illumina, CA, USA).