GSM9198079: Rat retina – Vehicle + Light damage (biol rep 4); Rattus norvegicus; RNA-Seq
Retinas were rapidly dissected under RNase-free conditions. Total RNA was extracted using the Qiagen RNeasy Mini Kit, following manufacturer's instructions, including on-column DNase I treatment. RNA quality was assessed by Agilent Bioanalyzer; samples with RIN ≥ 7 were used. RNA-seq libraries were prepared using the Watchmaker RNA Library Prep Kit (Twist Bioscience). Poly(A)-enriched mRNA was fragmented, reverse-transcribed, end-repaired, ligated to adapters, and PCR amplified according to manufacturer's protocol. Libraries were indexed and pooled for sequencing.