GSM8565767: HCT116_induced_APOBEC1-CNOT10-1; Homo sapiens; RNA-Seq
For mRNA-seq and ONT long-read sequencing, total RNAs were extracted using TRlzol Reagent (Invitrogen, 15596026) according to the manufacturer's protocol. For scRNA-seq, single cells were sorted into each well of 96-well plates containing 3 µL lysis buffer (0.3 µL 1% Triton X-100 (Sigma Aldrich, T9284), 0.05 µL 10 µM Oligo dT primer, 0.2 µL 10 mM dNTPs mix (Thermo Scientific, R0191), 0.03 µL 40 U/µL RNase inhibitor (Takara, 2313A), 0.4 µL 50% PEG8000 (Sigma Aldrich, 89510) and 2.02 µL ddH2O). 500 ng total RNA was used as input material for the mRNA-seq library construction (YEASEN, 12309ES96) following the manufacturer's protocol. Sequencing library was generated using 500 ng-2 µg total RNA was as input for ONT long-read sequencing library construction (ONT, SQK-PCS109). Single-cell RNA sequencing libraries were generated using the Smart-seq3xpress protocol.