GSM9369703: 1.EV.LPS.IFNg.STEMI.19.1; Homo sapiens; RNA-Seq
Following stimulation, cells were washed with PBS, centrifuged, and lysed using the lysis buffer from the miRvana miRNA Isolation Kit (Thermo Fisher Scientific) according to the manufacturer's instructions. Lysates were snap-frozen and stored at −80 °C until RNA extraction. RNA-seq libraries were prepared using the TruSeq Stranded mRNA Library Prep Kit (Illumina) following the manufacturer's protocol, including poly-A selection, RNA fragmentation, first- and second-strand cDNA synthesis, end repair/A-tailing, adapter ligation, and PCR enrichment. Libraries were quantified, pooled in equimolar amounts, and sequenced on Illumina HiSeq 3000/4000 instruments.