Frozen Yogurt with Green Banana Biomass Replicate 5
Frozen yogurt samples (FA and FB) were previously freeze-dried, whereas kefir samples were centrifuged and the supernatant discarded. Approximately 250 mg of each sample were used for total DNA extraction using the DNeasy PowerSoil Pro Kit (Qiagen, United Kingdom), following the manufacturer's protocol. The extracted DNA was quantified using the Qubit dsDNA kit (Thermo Fisher Scientific, Waltham, MA, USA) and submitted for sequencing by Novogene Co. (Durham, NC, USA). Amplification of the V3V4 region of the 16S rRNA gene was performed using the 341F and 806R primers, a region selected due to its broad taxonomic coverage of complex bacterial communities (Fadeev et al., 2021), on the Illumina NovaSeq 6000 platform (San Diego, CA, USA).