GSM9668326: PBMCs, mixed sample, batch B, sublibrary 3; Homo sapiens; RNA-Seq
Harvested cells from the in vitro stimulation were fixed using the Evercode™ cell fixation kit v2 (Parse Biosciences) according to the manufacturer's protocol. Single‑cell RNA‑seq libraries were generated using Evercode™ WT 100k Whole Transcriptome Kit v2 (Parse Biosciences) according to the manufacturer's instructions. Cells from multiple biological samples were combinatorially barcoded and pooled, such that each sequencing sub-library contained multiplexed material rather than a one-biological-sample-to-one-library relationship. In batch A, some biological samples were loaded across two wells in the sample loading plate, whereas others were loaded into a single well; in batch B, biological samples were loaded into one well each. After barcoding, eight sub-libraries were prepared per batch, each containing ~10,000 cells. The final libraries underwent standard library QC and quantification followed by sequencing on NovaSeq X Plus (Illumina) 25B 300-cycle flow cell lanes in 150 bp paired-end mode. Detailed sample-to-well assignments are provided in sample_metadata.tsv.