GSM9126400: HEL-Control; Homo sapiens; RNA-Seq
RNA  was harvested using MagZolTM Reagent（Axygen, USA） , adding chloroform, and centrifuging to collect the RNA. Total RNA was usde for the construction of sequencing libraries. Library preparation simplifies with a kit combining double-strand synthesis, end repair, and dA-tailing. mRNA is enriched with VAHTS mRNA Capture Beads（Vazyme, China）, then fragmented, and used for double-stranded cDNA synthesis. Libraries are quality-checked using the Agilent Bioanalyzer and Qubit.