GSM9666533: C1_3.fq.gz; Vaccinium corymbosum; RNA-Seq
Total RNA was extracted from ground blueberry fruit samples using the TRIzol reagent following the manufacturer's instructions. The total RNA quantity and purity were evaluated using a NanoDrop 2000 spectrophotometer and an Agilent 2100 Bioanalyzer. High-quality RNA samples with an RNA Integrity Number (RIN) ≥ 7.0 were used for subsequent cDNA library construction . The mRNA libraries were constructed for each sample of Populus simonii × P. nigra under alkaline stress, and sequencing work was carried out using the Illumina Novaseq platform to obtain the corresponding sequencing data. After sequencing was completed, quality assessment of the raw data was conducted.