16S Peat: SPRUCE
Peat samples were flash frozen in the field and stored at -80C. DNA was extracted from peat soil samples using the Qiagen PowerSoil DNA Kit according to the manufacturers instructions. DNA quality was assessed with a Nanodrop spectrophotometer, quantified using a Qubit High-Sensitivity (HS) assay, and verified for integrity on an agarose gel. Peat samples collected from 20172018 were processed at the University of Illinois at Chicago, where the 16S rRNA gene (V4 region) was amplified using primers CS1_515F (5-ACACTGACGACATGGTTCTACA_GTGCCAGCMGCCGCGGTAA-3) and CS2_806R (5-TACGGTAGCAGAGACTTGGTCT_GGACTACHVGGGTWTCTAAT-3). Samples from 2019-2021 were sequenced at Georgia Institute of Technology using primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA-3) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT-3) with overhang adapters (Apprill et al. 2015; Caporaso et al. 2011). Barcoded amplicons were sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 kit to generate paired-end reads of 250 bp.