Evergreen.1 (CRX2015566; CRA030272)
Poly A-containing mRNA was enriched from total RNA using Oligo(dT) magnetic beads, and then fragmented to approximately 300 bp using ion fragmentation. Subsequently, double-stranded cDNA was synthesized using RNA as the template, with random primers and reverse transcriptase. The cDNA library was then enriched by PCR amplification, followed by size selection of the library, resulting in a final library size of approximately 453 bp.