GSM8385438: i4F-Nes, replicate 1; Mus musculus; RNA-Seq
Mouse brains at E15.5 were collected and sectioned in 300 μm under a vibratome in ice-cold Leibowitz medium with 5% FBS. The rostal region of cortex for both hemisphere were collected using a scalpel. The tissues were transferred into a dissociation Buffer, EBSS, and were subsequently dissociated into a single cell suspension with Worthington Kit and manually Papain dissociation system according to the manufacturer's instructions. For experiments utilizing the 10x Genomics platform, the following reagents were used: Chromium Single Cell Next GEM Single Cell 3' GEM kit v3.1 (PN-1000130), Library Construction Kit (PN-1000196), Chromium Next GEM Single Cell 3′ Gel Bead Kit v3.1 (PN-1000129) and Dual Index Kit TT Set A (PN-1000215),  according to the manufacturer's instructions in the Chromium Single Cell 3′ Reagents Kits v3.1 User Guide.