amplicon sequencing of fungi from a synthetic community
The ATCC Mycobiome Genomic DNA Mix was used for PCR in duplicate with 5.8S-Fun/ITS4-Fun primers to generate ITS amplicons. Duplicate PCR reactions were pooled after agarose gel electrophoresis confirmed successful PCR amplification for both replicates. Amplicons were sent to the Cornell BRC for sequencing with a v2 500 bp kit with 2x250 Paired Ends on the Illumina Miseq.