MinION sequencing
SQK-NBD114.24
Genomic DNA was extracted using the Monarch HMW DNA Extraction Kit for Tissue using the low-input protocol and sheared 15x using a G27 syringe. 400 ng of HMW gDNA were used as input for the Native Barcoding Kit (SQK-NBD114.24) with barcode NB10. Libraries were then prepared as described by Oxford Nanopore Technologies and run for 72 h on a R10.4.1 flow cell (FLO-MIN114) in a MinION Mk1C. Basecalling and demultiplexing was performed using Dorado v0.5.0 (super accuracy model, v4.3.0) with the --barcode-both-ends option.