GSM8384939: LNCaP_CON, ETV5, ChIP; Homo sapiens; ChIP-Seq
Cells were fixed 1% formaldehyde for 20 minutes. After cell lysis, chromatin was sonicated using a Bioruptor Pico sonicator (Diagenode). ChIP-seq library construction was performed using the NEBNext ChIP-Seq Library Prep Master Mix Set (NEB, E6240) following the manufacturer's instruction with modifications. The libraries were sequenced using the Illumina HiSeq4000 platform with 100 bp paired-end reads.