GSM9369942: ISO-seq PARN CKO rep2; Mus musculus; OTHER
The spleen was isolated from 8-week old mice and disrupted in PBS  containing 2% fetal bovine serum. Remove aggregates and debris by passing cell suspension through a 70 μm mesh nylon strainer. The untouched and highly purified B cells were isolated from mouse splenocytes by immunomagnetic negative selection following the manufacture's protocol for EasyStepTM mouse B cell isolate kit (Stem cell, Catalog # 19754). ISO-seq was performed by Guangzhou Epibiotek Co., Ltd. The library is obtained by connecting sequencing adapter to the amplified cDNAs. The concentrations were loaded onto the R10.4.1 sequencing chip and perform sequencing on the PromethION sequencer (Oxford Nanopore Technologies, Oxford, UK) for 48 h.