Corynespora cassiicola treated with DMSO for 36 hours
The mycelium plug (diameter 4 mm) at the edge of Corynespora cassiicola colony 7d was transferred into PDB medium with DMSO, and inoculated in an incubator at 25 for 36 h. The RNA of the total sample was isolated and purified by TRIzol (thermofisher, 15596018) according to the operation scheme provided by the manufacturer. Then the quantity and purity of total RNA was controlled by NanoDrop ND-1000 (NanoDrop, Wilmington, DE, USA) and the integrity of RNA was detected by Bioanalyzer 2100 (Agilent, CA, USA). The concentration was >50ng/L, RIN value >7.0, and total RNA>4g.