GSM9455459: Cal27, control treatment, sample 3; Homo sapiens; OTHER
Genomic DNA isolation was performed using QIAamp DNA Blood Midi kit (Cat# 51104. Qiagen, Hilden, Germany) and sgRNA sequence were amplified using a PCR reaction according to the manufacturer's protocol (Addgene/Broad Institute) with maintenance of ~400x coverage of the human Kinome library.  All PCR reactions were performed using Ex Taq DNA polymerase (Clontech, RR001A). Samples were sent to the Yale Center for Genome Analysis (RRID:SCR_027324).  Sequencing was performed with NovaSeq paired-end 100bp reads (Illumina NovaSeq X, RRID:SCR_024569).