Archaeal 16S rRNA gene from rumen cultures
DNA extracted using bead beating and DNA Stool kit (Quiagen). PCR amplification of V3-V4 regions of archaeal rRNA gene. Illumina P5 and P7 adaptors attached to Illumina Nextera XT Index Kit v2 indices. Mixture was loaded in the Illumina platform at 2  600 Illumina paired forward and reverse cycles run with FastQ.