GSM8751801: Wild-type female 12010, Oocyte B; Mus musculus; RNA-Seq
Denuded oocytes were pipetted into 10.5 µL of Lysis buffer from the SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (Takara Bio). Performed under a stero microscope to confirm lysis of the oocyte occurred. Amplification was performed according to manufacturer instructions. Libraries were prepared with the ThruPLEX® DNA-seq Kit (Rubicon Genomics) according to manufacturer instructions