Mertk-cKO-injured
Total RNAs of microglia were extracted and reverse-transcribed using a template-switching oligo (TSO; BGI Genomics). cDNAs underwent pre-amplification and tagmentation to add sequencing adapters, and the index PCR was performed to incorporate sample-specific barcodes. The libraries were purified using VAHTS DNA Clean Beads (Vazyme) and assessed for quality and concentration using the Qubit (Thermo Fisher Scientific) and the Bio-Fragment Analyzer (BiOptic). The libraries were sequenced on the MGI DNBSEQ-T7 platform using 150 bp paired-end reads.