GSM9421551: neonatal sample small 9; Mus musculus; RNA-Seq
Total RNA was extracted from 30-50 hand-picked islets isolated from C57BL/6J mice at P5 (neonatal) and 2.5 months (adult) using the RNeasy Micro Kit (Qiagen). cDNA libraries were prepared from 250 ng total RNA using the KAPA HyperPrep Kit with RiboErase (HMR) (Kapa Biosystems) using manufacturer's instructions. Libraries were validated using the Agilent Bioanalyzer and quantified with the Qubit High Sensitivity DNA Assay (Thermo Fisher Scientific).