Whole genome sequence of Klebsiella pnuemoniae: pus swab from surgical wound of adult male
Libraries were constructed in alignment with whole genome sequencing recommendations of QIAseq FX DNA library kit from Qiagen. Briefly, 150 ng of DNA was used for fragmentation and end repaired, followed by adapter ligation, removal of excess adapter using AMPure XP purification beads, PCR enrichment of adapter ligated DNA and clean-up of library products as instructed by the manufacturer. The cleaned libraries were quantitated on Qubitflurometer and appropriate dilutions loaded on a HS D1000 screen tape to determine the size range of the fragments and the average library size.