GSM9155388: AML-12 cells, BSA_1; Mus musculus; RNA-Seq
Total RNA was extracted using Trizol according to the manufacturer's recommendation. High-quality RNA with 260/280 and 260/230 ratios higher than 1.8 were used. RNA integrity was assessed by gel electrophoresis or bioanalyzer At least 100 ng of RNA was used to generate cDNA libraries using the MERCURIUS kit (Alithea Genomics). Transcriptome analysis was performed by Alithea Genomic using the Bulk RNA Barcoding and sequencing (BRB-seq). Libraries were sequenced on Illumina NovaSeq 6000 platform at a depth of 8 million raw reads per sample.