R1.MidSfac
The V3-V4 region of the bacterial 16S rRNA gene was amplified by PCR using the primer pair 341F (5'-CCTAYGGGRBGCASCAG-3') and 805R (5'-GGACTACNNGGGTATCTAAT-3'). PCR products were purified using magnetic beads and used to construct sequencing libraries following the standard amplicon library preparation protocol. Paired-end sequencing was performed on the Illumina NovaSeq platform.