GSM8567235: RBf17, LN, wk0postATI, aIL10_aPD1, CA-vDNAhi, ATAC; Macaca mulatta; ATAC-seq
Lymph node mononuclear cells (LNMCs) nuclei were isolated according to the manufacturer's instructions using Chromium Nuclei Isolation Kit (10x Genomics, Pleasanton, CA). Single-cell Multiome ATAC and Gene Expression (GEX) libraries were prepared using the Chromium Single Cell Multiome ATAC + Gene Expression platform (10X Genomics). 8000 nuclei were used in each sample. Isolated nuclei were transposed and partitioned into Gel Beads-in-emulsion (GEMs) using the 10x Chromium Controller and Next GEM Chip J. ATAC and GEX libraries were generated from the same pool of pre-amplified transposed DNA/cDNA. Representative traces and quantitation of both libraries were determined using Bioanalyzer High Sensitivity DNA Analysis (Agilent, Santa Clara, CA).