Effective requesting method to detect fusion transcripts in chronic myelomonocytic leukemia RNA-seq
Effective requesting method to detect fusion transcripts in chronic myelomonocytic leukemia RNA-seq
Peripheral blood mononuclear cells were separated by Ficoll-Hypaque density gradient and preserved in RNA Later Total RNA was isolated using mini RNeasy kit (Qiagen) according to the manufacturer instructions. The quantity and quality of extracted RNA was evaluated using the NanoDrop ND-1000 spectrophotometer and the Agilent 2100 BioAnalyzer.  Libraries have been prepared using the “TruSeq Stranded Total RNA Gold” Kit from Illumina.