Transcriptome sequencing of BAB10-KIN1
We isolated total RNA using the RNAqueous kit (AM1912, ThermoFisher Scientific). We digested genomic DNA using the TURBO DNA-free kit (AM1907, ThermoFisher Scientific) according to the manufacturers instructions and removed DNase using DNase Inactivation Reagent. To prepare stranded libraries (orientation: RF), the NEBNext Ultra II Directional RNA Library Prep Kit Total RNA was used, applying double the normal rounds of PolyA selection prior to cDNA synthesis to reduce the excess of bacterial RNA. The forward adapter sequence was AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC and the reverse was AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT.