Illumina paired-end sequencing of Bacillus paralicheniformis TB197 genome: rhizospheric soil isolate from Sonoran desert
Genomic DNA was extracted from Bacillus paralicheniformis TB197 using a standard soil bacterial DNA extraction protocol. Paired-end sequencing libraries were prepared using Illumina TruSeq DNA library preparation kits following the manufacturers instructions. DNA was fragmented, end-repaired, A-tailed, and ligated to Illumina adapters. Libraries were amplified by limited-cycle PCR, quality-checked, and sequenced on an Illumina NovaSeq 6000 instrument using Sequencing by Synthesis (SBS) chemistry with paired-end reads of 151 bp. Whole-genome resequencing was performed to facilitate variant identification and gene annotation.