GSM8567393: stroma-rich AM tumoroid (normal fibroblast), 32hr; Homo sapiens; RNA-Seq
Primary tumor cells and cancer-associated fibroblasts were isolated from ameloblastoma patients. Normal fibroblasts were isolated from normal tissue adjacent tumors. Primary tumor cells were subjected to tumoroid culture in Matrigel. After tumoroid formation (7d), normal fibroblasts or cancer-associated fibroblasts were used to establish the stroma-rich tumoroid. Dexamethasone (100nM) was used for the synchronization of circadian rhythm in stroma-rich tumoroids. Matrigel was washed out with ice cold advanced DMEM,and the stroma-rich tumoroids were collected at the time-point of 0h, 8h, 16h, 24h, 32h, 40h, 48h after synchronization.The total RNA was extracted with TRIzol. RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.