GSM8853261: Multiome, Intact, ATAC; Mus musculus; ATAC-seq
Four to eight limbs from different time points (6, 24, 72 hpa) were pooled into an Eppendorf tube, snap-frozen on dry ice, and stored at -80°C until use. Single nuclei dissociation was conducted using a modified version of the 10x Genomics protocol (CG000365 Rev B). Single-cell Gene Expression libraries and ATAC libraries were then prepared using the Chromium Next GEM Single Cell Multiome ATAC + Gene Expression v1.0 (PN- 1000285) following the manufacturer's instruction (protocol CG000338 Rev F).