The complete plastid genome and 45S rDNA sequences of Murdannia medica (Lour.) D.Y. Hong
Genomic DNA libraries were prepared using the Illumina DNA Prep kit (formerly Nextera DNA Flex) following the manufacturers protocol. Briefly, 100 ng of high-molecular-weight DNA underwent on-bead tagmentation to simultaneously fragment and adapter-ligate the samples. The resulting fragments were amplified and tagged with Unique Dual Indexes (UDIs) via PCR to prevent index hopping. Library quality and fragment size distribution were verified using an Agilent Bioanalyzer, and final quantification was performed via qPCR. Libraries were then pooled and sequenced on an Illumina NovaSeq 6000 platform with 150 bp paired-end reads