MC38 CRISPR capture guide
MC38 cells stably expressing ABEmax were generated by lentiviral transduction followed by selection with 500 g/mL hygromycin, yielding the final MC38-ABE cell line. The MC38-ABE cells were transduced with varying titers of lentivirus at a low multiplicity of infection (MOI = 0.3) to ensure single sgRNA integration, using 10g/mL polybrene to enhance infection efficiency. At 24 hours post-infection, the medium was replaced with fresh culture medium containing 2 g/mL puromycin for a 48-hour selection period. Then, 8 days post-infection, cells were partitioned into droplet emulsions using the Chromium Controller with Chromium GEM-X Single Cell 5' Gell Beads v3, targeting a recovery of 20,000 cells per lane in accordance with the manufacturers protocol.