Control2
The metagenomic samples in this study were derived from rumen fluid. DNA was extracted from the samples using the E.Z.N.A. Soil DNA Kit, following the manufacturer's protocol. Subsequently, sequencing libraries were constructed using the NEXTFLEX Rapid DNA-Seq kit. Sequencing was performed on the Illumina NovaSeq 6000 platform, yielding paired-end reads of 150 base pairs in length. The raw read data underwent quality control, host removal was conducted using TBWA, and assembly was performed using MEGAHIT v1.1.2.