GSM9045554: Cal27_H3K36M_OE_HiC; Homo sapiens; Hi-C
C3H10T1/2 mouse mesenchymal stem and HNSCC cells (two million cells per sample) were crosslinked and processed using the Arima-HiC+ kit and following the Arima Genomics HiC protocol for Mammalian Cell Lines (A510008). Proximally-ligated DNA was fragmented to an average size of 400 bp using the BioRuptor UCS-300 at low intensity for 10 cycles (30s ON, 90s OFF). Library preparation of fragmented proximally-ligated DNA was performed using the NEBNext® Ultra™ II DNA Library Prep Kit (E7645S), NEBNext Multiplex Oligos for Illumina (E6440S), and the Arima HiC+ Kit according to manufacturer protocols. Hi-C libraries were sequenced by the McGill University Genome Centre at 150 bp paired-end reads using the Illumina NovaSeq 6000.