GSM9127992: Injection_control_1; Homo sapiens; OTHER
Genomic DNA from sorted cells was extracted using the Puregene Cell Kit and quantified with a NanoDrop ND-1000 spectrophotometer. sgRNA inserts were amplified via targeted and nested PCR using primers with Illumina adaptors. Libraries were sequenced on the Illumina MiSeq platform with 50 bp single-end reads. Illumina MiSeq