GSM8987210: Arabidopsis, gcn5-7_H3K9K14ac, 12DAG, Rep1; Arabidopsis thaliana; ChIP-Seq
The plants were cross-linked in a 1% formaldehyde solution under intermittent vacuum for approximately 10 minutes..After nuclear lysis,chromatin was sonicated using using Bioruptor® Pico with the following conditions:sonication ON for 10 seconds, sonication OFF for 30 seconds, 14 cycles, using 1.5-ml tubes at 4°C. ChIP-Seq libraries were prepared using Ovation® Ultralow System V2 Library Preparation Kit (PART NO. 0344, 0344NB).