RAD-seq. Oak Sample_058
Briefly - For the sampled oak populations, we extracted DNA from the collected leaves using DNeasy plant pro kit (QIAGEN), following a slightly modified version (somewhat longer homogenization time for tough leaves) of the manufacturers instructions. To assess DNA concentration and quality in the extracted DNA samples, we used a Qubit 2.0 fluorometer and a NanoDrop 2000 spectrophotometer. The extracted DNA was digested using HF EcoRI restriction enzyme - settings 37.0 C 2 hours, 65.0 C 20 minutes, and 5 C for 4 hours (NewEngland Biolabs), which was followed by a second DNA quantity and quality check with Qubit and gel electrophoresis. The samples were then sent to the SciLife laboratory in Stockholm, where they were sequenced using Illumina NovaSeq6000.