Tagmentation-based tag integration site sequencing of Nicotiana benthamiana transformed only with the payload construct and not the R2Tg ribonucleoprotein
Genomic DNA was mixed with Tn5 Transposase loaded with adaptors. Tagmented samples were purified and amplified with a nested PCR using insert specific primers and primers targeting the Tn5 adaptors. Amplicons were indexed prior to multiplexed sequencing.