GSM9097613: San1_tile3_30_rep1; Homo sapiens; OTHER
Plasmid DNA was extracted from 9 OD units of yeast cells using the ChargeSwitch Plasmid Yeast Mini kit (Invitrogen). Tiled DHFR indel libraries comprised wild-type DHFR; a synonymous (silent) variant at each position; a nonsense (stop codon) mutation at each position; a single glycine insertion at each position; and a single amino acid deletion at each position. The libraries were cloned into pAG415GPD-ccdB.