GSM8887406: EW-1 cell line, WT, SOX2 (ChIP); Homo sapiens; ChIP-Seq
Cells were fixed for 10 minutes with 1% methanol-free formaldehyde and the chromatin was sonicated for 20 cycles (30 seconds on, 30 seconds off) at the "high" setting. ChIP-Seq experiment was performed for IKZF2, POU3F2, SOX6, FLI1 and H3K27ac using Diagenod kit. Respective inputs were used as normalization controls for all models. ChIP sequencing libraries were generated using the TruSeq ChIP library preparation kit (Illumina) and sequenced on an Illumina HiSeq 2500 or NovaSeq 6000 (single -end, 100 bp). 