GSM9201446: 4 feature barcode multiplexed samples group D; Homo sapiens; OTHER
Library was prepared using Chromium Single Cell 3ʹ version CG000769 rev A protocol (10x Genomics) with on chip multiplexing technology. Cellular mRNA and antibody-derived oligos were reverse-transcribed and indexed with a shared cellular barcode. Indexed cDNAs were then pooled and amplified by PCR according to the Chromium Single Cell 3' version  CG000769 rev A protocol (10x Genomics) and with specific primers amplifying the antibody-derived tags. SPRI bead size selection was performed in order to separate both the mRNA-derived cDNA and the antibody-derived tagged cDNAs. For the mRNA derived cDNA library preparation, we further proceeded according to the manufacturer's instructions for Single Cell 3' version CG000317 rev B protocol (10x Genomics). Libraries were equimolar pooled to obtain at least 20,000 read pairs per cell and 5,000 read pairs for feature barcode part after sequencing on Illumina Novaseq X (100 cycles cartridge).