Genome Sequencing of Rhodopseudomonas palustris R1
R. palustris R1 strain (JCM 2524) was purchased from the Microbe Division in Riken BioResource Research Center. A single colony was extracted and grown over 72 hours at 30C in an aerobic environment (AnaeroPack  aerobic; MITSUBISHI GAS CHEMICAL CO. INC) with light on a M95 medium (1), shaking at 160 rpm, and harvested at an OD600 of around 0.3. The genomic DNA was extracted using the NucleoBond HMW DNA for High Molecular weight DNA (MACHEREY-NAGEL) according to the manufacturers instructions. Long-read sequencing library was prepared using the Rapid Barcoding Kit 24 V14 (product no. SQK-RBK114.24, Oxford Nanopore Technologies), and was sequenced with a GridION device (Oxford Nanopore Technologies) in a FLO-MIN114 flow cell. Subsequently, the reads were base-called and demultiplexed by Guppy version 6.5.7 (Super-Accurate Mode).