GSM9197438: mouse subiculum neurons,Nr4a2 C57_2_R1,Day7; Mus musculus; OTHER
RNA-seq: Total RNA was extracted from subiculum tissues using TRIzol (Invitrogen) following the manufacturer's instructions. RNA integrity was assessed on an Agilent 2100 Bioanalyzer; samples with RIN ≥ 7.0 were used.  CUT&Tag: 1×10^5 cells were processed following the CUT&Tag protocol (Kaya-Okur et al., 2019) with minor modifications. Cells were bound to ConA-coated magnetic beads, incubated with primary antibody (1:50) overnight at 4°C, then with secondary antibody (1:100) for 1 h at RT prior to pA–Tn5 binding and tagmentation. RNA-seq: mRNA was purified with poly‑T oligo magnetic beads, fragmented, and reverse‑transcribed. Libraries were end‑repaired, A‑tailed, adapter‑ligated, PCR‑amplified, purified with AMPure XP, QC‑checked on an Agilent 2100 Bioanalyzer, and sequenced on an Illumina NovaSeq 6000 (PE150).  CUT&Tag: DNA fragments were amplified using NEBNext HiFi 2× PCR Master Mix with universal i5 and barcoded i7 primers. Cycling: 72°C 5 min; 98°C 30 s; 14 cycles of 98°C 10 s, 63°C 30 s; 72°C 1 min. Post‑PCR cleanup with AMPure XP, TapeStation QC, and sequencing on Illumina NovaSeq 6000 (PE150).