RNA-seq  of levofloxacin stress in Aquatica leii
Total RNA was isolated using the TRIzol Reagent (Invitrogen Life Technologies) as per the manufacturers recommendations. RNA integrity and removal of genomic DNA were obtained by 1.5% agarose gel electrophoresis, as described previously [7]. Subse-quently, the concentration, quality, and integrity of the RNA were determined via a Ther-mo Scientific NanoDrop 2000 (Thermo Scientific, Waltham, MA, USA), a Qubit2.0 Fluo-rometer (Thermo Scientific, Waltham, MA, USA) and an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Sequencing libraries were derived from 1g of total RNA using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Bi-olabs, Ipswich, MA, USA), and were sequenced to generate 150 bp paired-end reads on the Illumina Novaseq 6000 platform by Shanghai Personal Biotechnology Cp., Ltd.