GSM8709839: S. anginous KH1 grown in artificial saliva medium, sample 2; Streptococcus anginosus; RNA-Seq
Total cellular RNA was isolated from KH1 cultures at OD600 = 0.3  and further purified using the RNeasy kit (Qiagen). Ribosomal RNA was removed from the RNA preparation using RiboMinus transcriptome isolation kits (Invitrogen). PCR was performed with primers specific for SAGKH1_01193 to ensure the preparations were free of chromosomal DNA. The RNA library was constructed using a SureSelect XT HS2 mRNA library preparation kit (Agilent, USA).