biosamples are from the bioreactor on day 182 (CRX824641; CRA012893)
Total microbial genomic DNA samples were extracted using the OMEGA Mag-Bind Soil DNA Kit (M5635-02) (Omega Bio-Tek, Norcross, GA, USA), following the manufacturer's instructions, and stored at -20degreeC prior to further assessment. The quantity and quality of extracted DNAs were measured using a Qubit 4 Fluorometer, with WiFi: Q33238 (Qubit Assay Tubes: Q32856; Qubit 1X dsDNA HS Assay Kit: Q33231) (Invitrogen, USA) and agarose gel electrophoresis, respectively. The extracted microbial DNA was processed to construct metagenome shotgun sequencing libraries with insert sizes of 400 bp by using Illumina TruSeq Nano DNA LT Library Preparation Kit. Each library was sequenced by Illumina NovaSeq platform (Illumina, USA) with PE150 strategy at Personal Biotechnology Co., Ltd. (Shanghai, China). submitted_instrument_model: Illumina NovaSeq 5000