GSM8715342: MUTZ3 - RNAseq - SH1147 - 48HRS - REP1; Homo sapiens; RNA-Seq
Total RNA was isolated by phenol chloroform extraction followed by DNase digestion. The SuperScript II Reverse Transcriptase kit (Invitrogen) was used for synthesis of cDNA. The cDNA was sheared with the Covaris device and further processed according to the TruSeq RNA Sample Preparation v2 Guide (Illumina)