IL-27Ra Promotes the Progression of Neonatal Necrotizing Enterocolitis
IL-27Ra Promotes the Progression of Neonatal Necrotizing Enterocolitis
Intestinal tissue samples were collected from 7 neonates with surgically confirmed necrotizing enterocolitis (NEC). The control group consisted of 7 gestational age-matched infants diagnosed with congenital intestinal atresia. Total RNA was extracted using the TRIzol reagent (Invitrogen, Cat No. 12183555) following the manufacturer's protocol.  Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Illumina, Cat No. 20040526). Sequencing libraries were prepared using the TruSeq Stranded Total RNA Library Prep Kit (Illumina, Cat No. RS-122-2001) according to the manufacturer's instructions. RS-122-2001) according to the manufacturer's instructions.