GSM9455971: Hep3B cells, Hypoxia-1; Homo sapiens; RNA-Seq
RNA was harvested using TRIzol Reagent. A total amount of 3 µg of RNA per sample was used for the construction of sequencing libraries. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.