ALLM2 (CRX2028248; CRA030551)
First, whole-body samples were homogenized. After RNA extraction, residual genomic DNA was removed. RNA quality was assessed using a Nanodrop 2000 spectrophotometer (Thermo Scientific, USA), with the OD260/280 ratio of each RNA sample ranging from 1.8 to 2.0; RNA structural integrity was verified by 2.0% agarose gel electrophoresis. Libraries for small RNA sequencing were constructed using a Small RNA-Seq Library Preparation Kit (Lexogen, Austria). Finally, the prepared small RNA sequencing libraries were quality-assessed via an Agilent 2100 Bioanalyzer (Agilent, USA) and an ABI StepOnePlus Real-Time PCR System (Applied Biosystems, USA). A total of 12 small RNA sequencing libraries were generated, including ALA1-3, ALL1-3, LPA1-3, and LPL1-3. These libraries were sequenced with single-end 50 bp reads on the HiSeq 2500 platform (Illumina, USA) at the Beijing Genomics Institute (BGI, Shenzhen, China) under BGI's quality control.