GSM9666150: fzf1 cyanamide rep 1; Saccharomyces cerevisiae; MNase-Seq
Cells were resuspended in spheroplast buffer, and 10% cross-linked Schizosaccharomyces pombe cells were added to each sample as a spike-in. Cells were treated with 2 mg of zymolyase for 15 mins at 25°C with rotation, then digested with 60 units of micrococcal nuclease (MNase) and 30 units of ExoIII for 11 minutes at 37°C. Residual proteins and RNAs were removed, and DNA was purified with a QIAGEN MinElute PCR Purification Kit. Libraries were prepared with NEB DNA Ultra II kit, and sequenced on an Illumina NextSeq.