RNA-seq of adult Platynereis dumerilii midgut
Adult Platynereis dumerilii of the Heidelberg strain were dissected to isolate pure digestive organs. Fresh digestive organ tissue was homogenized using TRIzol, and RNA was purified following the manufacturer's instructions. The quality of the extracted RNA was assessed using the Agilent Fragment Analyzer 5200 to evaluate RNA integrity, and the Qubit 4 Fluorometer to measure RNA concentration. Experiments were conducted in three biological replicates. Bulk RNA sequencing was performed using the Illumina HiSeq 2500 platform with 148 bp paired-end deep sequencing, carried out by the NGS High Throughput Genomics Core at Academia Sinica.