GSM9666408: HT rat hippocampus biological replicate 1; Rattus norvegicus; RNA-Seq
The whole hippocampus was rapidly dissected, placed in RNAlater, and homogenized. Total RNA was extracted using TRIzol reagent followed by purification with the PureLink RNA Micro Kit according to the manufacturer's instructions. Poly(A)+ RNA was purified using oligo(dT) magnetic beads. cDNA libraries were prepared with the NEBNext Ultra II RNA Library Prep Kit. Library quality was assessed using Agilent BioAnalyzer / High Sensitivity DNA Kit, and sequencing was performed on the Illumina HiSeq1500 platform to generate single-end 50-bp reads.