ATAC-seq of C. briggsae contain Cni-xol-1 rep1
Embryos were collected and treated with bleach, followed by removal of eggshells using Yatalase. After washing with DPBS, embryos were resuspended and dissociated into single cells with Pronase E. For each biological replicate, approximately 1.5 million cells were obtained.Cells were lysed on ice for 10 min in lysis buffer (10 mM Tris-HCl, pH 7.4; 10 mM NaCl; 3 mM MgCl; 0.05% digitonin). Nuclei were then subjected to tagmentation using Tn5 transposase (TruePrep DNA Library Prep Kit V2 for Illumina, Vazyme TD501). DNA fragments were purified with the MinElute PCR Purification Kit (Qiagen) and amplified by 10 cycles of PCR using NEBNext High-Fidelity 2 PCR Master Mix. Final libraries were sequenced on the DNBseq-T7 platform.