DNA-Seq of soil PreApp InH Rep3
DNA was extracted using the Qiagen PowerSoil kit, quantified with a Qubit 4 fluorometer, and sequenced (2150 bp) on an Illumina MiSeq. Reads were quality-checked (FastQC), trimmed (Trimmomatic), and assembled (MEGAHIT). Genome bins were reconstructed (MetaBAT), assessed for quality (CheckM), and those with 50% completeness and <10% contamination retained. Taxonomy was assigned using GTDB-Tk, and a maximum likelihood phylogeny was built with GToTree. Antibiotic resistance genes were identified in both genome bins and unbinned contigs using ABRicate.