GSM9018399: tumor bearing GFP control; Mus musculus; RNA-Seq
After liver digestion via collagenase perfusion as described, the supernatant from the first centrifugation was used to enrich for F4/80 expressing cells using magnetic anti-F4/80 microbeads according to manufacturer's instructions (Miltenyi Cat. No. 130-110-443). Live cells were enriched using a dead cell removal kit (Miltenyi Cat. No. 130-090-101) and used for sc-RNA-Seq. Cells isolated from 1 individual tumor bearing control and 1 individual tumor bearing Glul-KO mouse were sequenced in separate wells on the flow cell. At minimum, 7,500 cells were sequenced at 20,000 reads per cell. Single cell sequencing was performed on NextSeq 2000 and 3PrimeV2 library prep was conducted according to standard methods. 10X snRNA-seq 3PrimV2 library preparation