pig_sc_s2 (CRX826590; CRA012950)
Cells were selected under the microscope, single cells with complete cell morphology and good activity were selected in principle, and the target cells were sucked out of the initial suspension with an oral straw, washed into the PBS-BSA solution drops, and then transferred to the Malbac lysate provided by the company. The samples were directly amplified by Malbac method, and the amplified products with the length of about 300 ~ 2kb were obtained. The amplified products were purified and recovered by Beckman Ampure XP magnetic beads.