Er25-Li_S28_Liver_Sruber
After each predation experiment (ingestion of A. trivittata or S. ruber) or a 5-day fasting period (control), E. reginae individuals were euthanized. Digestive tissues (tongue, stomach, liver, gut) were collected in the field, stored in RNAlater, and transferred to -80 C storage. Total RNA was extracted using the Monarch Total RNA Miniprep Kit (NEB Biolab) from <10 mg tissue, following homogenization with a PowerLyzer 24 bead beater (MO BIO Laboratories). RNA quantity and quality were assessed using the Qubit RNA HS Assay Kit (Thermo Fisher Scientific) and Bioanalyzer RNA Analysis (Agilent). Samples with RIN 7 (except a few irreplaceable samples) were selected for library prep using the Watchmaker mRNA Capture Kit with poly(A) selection. Libraries from low-RIN samples received seven extra PCR cycles. Libraries were sequenced to generate approximately 30 million 150 bp paired-end reads per sample on an Illumina NovaSeq X 10B flow cell.