GSM8565936: Hi-C, WAPL-AID cells, RAD21-FKBP_auxin_dTAG-8nM-6h, rep1; Mus musculus; Hi-C
kit manufacturer protocol Arima Hi-C cat#A510008. Hi-C was performed using the kit from Arima Genomics as per manufacturer's instructions (202103-1577). Briefly, mESCs were dissociated using TrypLE, resuspended in culture medium and counted. 5-10 million cells were spun down at 100g for 5 minutes at RT. Supernatant was discarded and the cell pellets were resuspended in 5 ml of 1X PBS. Cells were crosslinked using 2% formaldehyde (diluted from 37% formaldehyde - Electron Microscopy Sciences 15686), mixed by inverting the tubes 10 times and incubated at RT for 10 minutes. Quenching was done using the Stop solution 1 provided with the kit, mixed by inverting the tubes 10 times and incubated at RT for 5 minutes. Samples were then incubated on ice for 15 minutes, spun down at 100g for 5 minutes at 4°C. After discarding the supernatant, cells were resuspended in cold 1X PBS, counted, aliquoted at a density of 1x106 cells/tube and spun down at 100g for 5 minutes at 4°C. Supernatant was discarded, cell pellets were snap frozen on dry ice and stored at -70°C.