GSM9745465: ipscTG, control, rep1, lane1; Homo sapiens; RNA-Seq
iPSC-TG nociceptors were washed in PBS with 0.04% BSA and re-suspended at a concentration of ~1500cells/μl before capturing single cells in droplets on Chromium 10x Genomics platform. Library generation for 10x Genomics v2 chemistry was performed following the Chromium Single Cell 3ʹ Reagents Kits User Guide:  CG00052 Rev B.  Quantification of cDNA was performed using Qubit dsDNA HS Assay Kit (Life Technologies Q32851).  Quantification of library construction was performed using Qubit dsDNA HS Assay Kit (Life Technologies Q32851) and high-sensitivity DNA tapestation (Agilent.  5067-5584). Libraries were sequenced on Illumina HiSeq4000 platform to achieve an average of 42,000 reads per cell.