GSM8989405: H1048 with sgNOTCH2_sgNT_Rep2; Homo sapiens; RNA-Seq
RNA was extracted using Quick-RNA™ Miniprep kit (Cat # R1055, Zymo Research, CA, USA) including a DNase digestion step according to the manufacturer's instructions and RNA sequencing was performed. For 1), the libraries for RNA-seq are prepared using NEBNext Ultra II non-stranded kit.  Paired end 150bp sequencing was performed on Novaseq6000 sequencer using S4 flow cell. For project 2), a strand-specific library was built, and paired-end 150bp sequencing was performed on a Novaseq6000 sequencer using an S4 flow cell.