GSM9665469: Tet2fl/fl PD-1+CD8+ TILs, biol rep 2; Mus musculus; Bisulfite-Seq
PD-1+CD8+ tumor-infiltrating lymphocytes (TILs) were isolated from liver metastatic tumors by enzymatic digestion and density gradient centrifugation. CD45+ cells were enriched by MACS separation, and PD-1+CD8+ TILs were sorted by flow cytometry using anti-CD45, anti-CD3ε, anti-CD8α, and anti-PD-1 antibodies on a BD FACSAria III cell sorter (BD Biosciences, RRID: SCR_016695). Genomic DNA was extracted from sorted cells using the QIAamp DNA Blood Mini Kit (Qiagen, Cat#51106). WGBS libraries were prepared using the post-bisulfite adaptor tagging (PBAT) protocol. Library yields were quantified using the Library Quantification Kit (Takara Bio Inc.) and amplified using PrimeStar Max DNA polymerase (Takara Bio, Cat#R045A).