RNA-Seq of Mylopharyngodon piceus: Juvenile 3 Eye
Total RNA was extracted using RNeasy kit (Qiagen), and the RNA quality and quantity were evaluated using Qiaxcel RNA kit. The RNA-Seq library was prepared from mRNA using TruSeq Stranded mRNA kit (Illumina), and the library was analyzed in paired-read sequencing for 100-bp with 50 million reads per sample in the NovaSeq 6000 S4 Flow Cell system (Illumina).