GSM9456971: colonic epithelial tissue, colitis recovered, rep 1; Mus musculus; OTHER
Colonic tissue was lysted using 0.1% Triton-X 100 and 20% glycerol and bound nuclei were bound to Concavalin-A beads prior to incubation with anti-Fos antibody (courtesy of M. Greenberg) overnight. Nuclei were washed. Incubated with secondary antibody, washed again and incubated with pAG-Tn5. Nuclei were then lysed with 0.1% SDS then quenched with 0.5% Triton-X 100. Purified DNA was PCR amplified using NEBNext 2X MasterMix with a unique i5 and i7 index pairing per sample. Final libraries were purified using Zymo DNA Clean & Concetrator Kit.