GSM9370094: PC3 parental control, replicate 3; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer's protocol. RNA integrity was assessed using the Agilent TapeStation system. 800 ng of high-quality RNA (poly(A)+) from each sample was used for library preparation. Poly(A) mRNA was enriched using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB E7490). Sequencing libraries were constructed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB E7765) with sample purification beads and NEBNext Unique Dual Index primers (E6440S). Libraries were sequenced on an Illumina NextSeq 2000 instrument using paired-end 100 bp reads.