GSM8887884: KO_CysF_female; Mus musculus; RNA-Seq
Cth+/+ (WT) and Cth-/- mice were fed with control or cystine free diet for 4 days.Subcutaneous adipose depots were collected, with lymph nodes removed, pooled and digested. Isolated cells were subjected to droplet-based 3' end massively parallel single cell RNA sequencing using Chromium Single Cell 3' Reagent Kits as per manufacturer's instructions (10x Genomics). The libraries were sequenced using a HiSeq3000 instrument (Illumina)