ChIP-Seq of GS115-delta0498-0498-His-40mM DTT
Chromatin was cross-linked with formaldehyde in cells, followed by sonication to shear DNA to 200-500 bp fragments. Immunoprecipitation was performed using an anti-His tag antibody to enrich DNA fragments bound by the 0498-His fusion protein. Cross-links were reversed, and the immunoprecipitated DNA was purified. A ChIP-seq library was constructed by end-repair, dA-tailing, adapter ligation, and PCR amplification, then sequenced on the [Illumina Nova PE150 ] platform with 150 bp paired-end reads.