GSM8816441: MS MS274; Homo sapiens; RNA-Seq
Total RNA was isolated from tissue using the RNeasy Plus Universal Mini Kit (Qiagen) according to the manufacturer's protocol with an additional DNAse digestion step using DNase I, RNase-free (Roche). 1ug of Total RNA was used for the construction of sequencing libraries. rRNA depletion was performed using the NEBNext rRNA Depletion V2 Kit (Human/Mouse/Rat) (NEB). RNA sequencing library preparation used the NEBNext Ultra II RNA Library Prep Kit for Illumina by following the manufacturer's recommendations (NEB).