GSM9420492: pEPSCs differentiated in LCDM; Sus scrofa; RNA-Seq
We lysed captured cells and released RNA was barcoded through reverse-transcription in individual single-cell gel beads in the emulsion (GEMS). In each droplet, complementary DNA (cDNA) was generated and amplified through reverse-transcription on a T100 PCR Thermal Cycler (Bio Rad) at 53 C for 45 min, followed by 85 C for 5 min and a hold at 4 C. Then, cDNA concentration and quality were assessed using Qubit Fluorometer (Thermo Scientific) and bioanalyzer 2100 (Agilent), respectively. According to the manufacturer's introduction, scRNA-seq libraries were constructed and sequenced to a depth of  24142 reads per cell on illumina platform by Annoroad Gene Technology Co., Ltd.24142