GSM9420939: SKOV-3 cisplatin-resistant, KDM5B knockout, H3K4me3 (ChIP); Homo sapiens; ChIP-Seq
Prior to cell lysis, cells were cross-linked with 1% formaldehyde for 7 minutes at 37°C to preserve protein-DNA complexes. Cells were lysed and chromatin was sheared to an average size of 200 bp to 500 bp using sonication for 5 min at 20% amplitude. DNAs were amplified and subjected to DNA library construction using Illumina DNA Prep Kit (Illumina, Inc., San Diego, CA, USA) following the manufacturer's instructions. The libraries were quantified using KAPA Library Quantification kits for Illumina Sequencing platforms according to the qPCR Quantification Protocol Guide (#KK4854, KAPA BIOSYSTEMS) and qualified using the D5000 Screen Tape (Agilent Technologies, Waldbronn, Germany).