GSM8851812: replicate 5 control WT zebrafish 48hpf total RNA; Danio rerio; RNA-Seq
Trizol Chloroform extraction of total RNA followed by Dnase I treatment, and Monarch RNA cleanup kit (T2030L). All samples were confirmed to have A260/A280 ratios between 1.8 and 2.0 on Nanodrop spectrophotometer. All samples were confirmed to have RNA integrity numbers of above 7 via Agilent Bioanalyzer 2100 Zymo-Seq RiboFree Total RNA library Kit (R30003)