Amplicons for soil microbes under ambient and drought treatment
The Drought Manipulation Experiment (DIGME) is a multi-site field study investigating microbial responses to altered precipitation across diverse ecosystem types, including drought and ambient treatments. Soil DNA was extracted from ~0.25 g of soil using the DNeasy PowerSoil Pro Kit (Qiagen). Bacterial communities were characterized by amplifying the V4 region of the 16S rRNA gene using universal primers 515F and 806R.