Whole-genome sequencing of Aeromonas veronii strain KMM289 (Illumina): adult threespine stickleback
Strain was streaked onto LB agar from original freezer stocks and incubated at 25 C overnight. Colonies were then inoculated in 5mL of LB media and incubated at 37 C at 200 rpm overnight. Genomic DNA was extracted using the DNeasy Blood and Tissue Kit (Qiagen, Germany) according to the manufacturer's instructions. DNA was sent to Plasmidsaurus for library preparation and hybrid whole-genome sequencing. Illumina libraries were prepared using the Illumina DNA Prep Kit (20060059). Sequencing was performed on NovaSeq X Plus (paired-end 2x150bp reads)