GSM9096694: SNAI1, HUVEC, IP-3; Homo sapiens; OTHER
Cells were harvested and subjected to CUT&RUN according to the Cell Signaling Technology (CST) protocol. Briefly, cells were immobilized on concanavalin A–coated magnetic beads, permeabilized, and incubated with specific antibody overnight at 4°C. Samples were then incubated with pA-MNase and activated by calcium addition. Digested chromatin was released and DNA purified using spin columns. DNA was used to prepare sequencing libraries following a standard protocol for low-input samples. DNA fragments were end-repaired, A-tailed, and ligated to sequencing adapters. Libraries were amplified by PCR, size-selected (typically 150–400 bp), and quality checked using the Agilent High Sensitivity DNA Kit.