GSM9700324: IV- SenNP+ CD8 T cells, Day 0 post exposure, batch 2; Mus musculus; RNA-Seq
Nasal cavity samples were digested in 5 g/l of Collagenase D and 1e6 U/l of Dnase for 30 minutes at 37°C. T cells were FACS sorted on a FACSAria as Viability Dye negative, intravital label (anti-CD3e) negative, Sendai-NP (Kb 324-332) positive, and CD8 (clone 53-6.7) positive. 10x 5' scRNA-seq libraries were generated using the Chromium Next GEM Single Cell 5' Reagent Kit v2 (10x Genomics 1000263) according to the instructions provided.