Macropharyngodon choati
Tissue samples (spleen, liver, and brain tissue) from six individuals were processed individually in separate extractions. For homogenisation, each sample was placed in a bashing tube containing 2.0 mm lysis beads (S6003) and 800 L of TRIzol (Zymo Research), then vortexed at maximum speed for 10 minutes (Vortex-Genie 2). The homogenate was centrifuged at 12,000  g for 5 minutes to remove tissue debris. Total RNA was extracted from the clarified supernatant using the Direct-zol RNA Miniprep Plus Kit (Zymo Research, CA, USA) following the manufacturers instructions. Library preparation for each sample (n=18) was prepared using the TruSeq Total RNA Library Preparation Protocol (Illumina). Ribo- Zero Plus Kit (Illumina) was employed for ribosomal RNA depletion and paired-end sequencing (150 bp) was conducted on the NovaSeq 6000 platform (Illumina)