2bRAD-M of Litopenaeus vannamei gill
Gill filament samples were collected from healthy and Yellow Gill Syndrome (YGS)-affected Pacific white shrimp (Litopenaeus vannamei) in culture ponds in Yancheng, Jiangsu, China. Total DNA was extracted and subjected to 2bRAD-M library construction. DNA was digested with BcgI, ligated with adapters, PCR-amplified with sample-specific barcodes, size-selected (~100 bp), purified, and sequenced on the Illumina NovaSeq 6000 platform (PE150). Microbial marker databases were constructed from >400,000 genomes for species-level identification and relative abundance estimation.