GSM9289398: L2/3 neurons, WT, E19, Male Rep2; Mus musculus; RNA-Seq
Cells were collected in kit lysis buffer and snap-frozen to store them until used. RNeasy® Plus Micro Kit (Qiagen, #74034) was employed for RNA extraction. Final concentration and quality were determined by microfluidics-based automated electrophoresis using a Bioanalyzer (2100 Bioanalyzer System) RNA libraries were generated in the Genomics Unit of the CNIC (Madrid, Spain). 300 pg of total RNA (RNA integrity number >7.5) were used to generate barcoded RNA libraries using the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs, #E6420) according to the manufacturer's instructions. Low Input RNA sequencing was performed using Illumina NextSeq 2000 (Illumina), and 60 nucleotides single-end reads were obtained