DNA-seq of ANME2d-O
One ng of DNA was used for Illumina library construction using the Nextera XT Library Preparation Kit (Illumina, San Diego, California, USA). The libraries were checked for quality and size distribution using the Agilent 2100 Bioanalyzer and the High sensitivity DNA kit (Agilent Technologies, Santa Clara, California, USA). Quantitation of the library was performed by Qubit using the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific Inc Waltham USA). Libraries were normalized, pooled and paired-end sequenced (2x 300bp) using the Illumina Miseq (Illumina) and the MiSeq Reagent Kit v3 (Illumina) according the manufacturers protocol.