GSM9197792: Mtb_H37Ra, Input, ChIP; Mycobacterium tuberculosis H37Ra; ChIP-Seq
Mix the sample with a 1% formaldehyde solution and crosslink it at 24°C for 20 minutes. Then, add 2.5 M glycine to a final concentration of 125 mM and shake at room temperature for 10 minutes to stop the crosslinking process. Spin the centrifuge at 4°C for 10 minutes, discard the liquid above, and gather the bacteria; then mix the bacteria in 20 ml of cold TBS (20 mM Tris-HCl pH7.5, 150 mM NaCl), spin the centrifuge at 4°C for 10 minutes, discard the liquid above, and gather the bacteria. Resuspend the bacteria in 2 ml of TBS buffer that has been chilled beforehand, then centrifuge at 4°C for 10 minutes, discard the supernatant, and gather the cell pellet. After that, the CHIP assay and sequencing were performed by E-GENE Tech Co., Ltd.