GSM9724121: hDF_2, RNA-seq; Homo sapiens; RNA-Seq
Total RNA was extracted from cell pellets and assessed for integrity on a Bioanalyzer 2100 (Agilent) prior to library construction at BGI. Libraries were prepared by BGI following the standard Transcriptome Resequencing protocol (BGI_Bioinformatics_Analysis_Report_for_Transcriptome_Resequencing, project F14FTSNCHT0026_HUMunsT): mRNA enrichment with oligo(dT) magnetic beads, fragmentation in fragment buffer to 200-500 bp, first-strand cDNA synthesis with random hexamer-primer, second-strand synthesis with buffer + dNTPs + RNase H + DNA polymerase I, double-strand cDNA purification with QiaQuick PCR extraction kit, end-repair and A-tailing, sequencing-adaptor ligation, agarose gel size selection and PCR amplification. Libraries were sequenced on an Illumina HiSeq 2000 as 90 bp paired-end reads.