GSM9664522: Human corneal epithelial cells cultured in defined special medium; Homo sapiens; RNA-Seq
Total RNA was extracted using the Qiagen RNA isolation kit (#74104) according to the manufacturer's protocol. RNA quality was assessed, and samples with RNA integrity number (RIN) >= 7.0 and OD260/280 ratios between 1.8-2.1 were used. RNA sequencing libraries were prepared from poly(A)-selected RNA using the TruSeq Stranded mRNA Library Prep Kit (Illumina).