PacBio HiFi long-read seq of the Antarctic springtail: 2023
A 15 g of DNA extract that has passed the quality control criteria was cut into 15 Kb or larger using Covariis g-TUBE, purified using 0.45X AMPure XP magnetic beads, and then measured the size of sheared DNA through bioanalyzer. After serial steps of DNA purifying and adapter ligation, the manufactured library of 2-5 g was placed in one lane of BluePippin 0.75% Gel, and electrophoresis was run to collect libraries over 9-13 Kb and 15 Kb.