GSM9632803: P10; Mus musculus; RNA-Seq
Following cortex dissection, formaldehyde fixed, and paraffin embedded (FFPE) neural tissue was dissociated for Chromium Next GEM Single Cell Fixed RNA Profiling following manufacturer protocol (CG000553; 10X Genomics), using manufacturer profiling reagent kit (CG00527; 10X Genomics) and manufacturer Chromium mouse transcriptome probe set v1.0.1. Library construction was performed following manufacturer protocol CG000527. Cells were fixed and permeabilized prior to library preparation. Gene expression was measured using probe pairs with unique probe barcodes for multiplexing, allowing pooling of multiple samples before partitioning. Samples were partitioned into Gel Bead-in-Emulsions (GEMs), where probe pairs were ligated and labeled with GEM barcodes and UMIs. Barcoded products were amplified and underwent sample index PCR to add Illumina adapters and dual indices (i5/i7). Libraries were purified, quality-checked, and sequenced on an Illumina platform. Libraries were generated using the 10X Genomics Chromium Flex Mouse Transcriptome Probe Set v1.0.1