GSM8988856: ZC3H7A/B-DD-RNA-Seq- Replicate 1; Homo sapiens; RNA-Seq
Flag/HA control, ZC3H7A-OE, or ZC3H7B-OE HEK293 cells (3 independent replicates each) were lysed in hypotonic buffer (5 mM Tris-HCl (pH 7.5), 2.5 mM MgCl2, 1.5 mM KCl, 1x EDTA-free protease inhibitor cocktail, 2 mM DTT, 200 U/ml RiboLock RNase Inhibitor, 0.5% (v/w) Triton X-100, and 0.5% (v/w) Sodium Deoxycholate), to isolate the polysomes. A 50 µg fraction of each lysate was collected for polyA RNA-Seq. Sequencing libraries were generated via the Illumina TruSeq RNA Sample Preparation Kit v2. The RNA-Seq libraries for the ZC3H7A/B-DD and Parental cells were generated using Illumina compatible protocols by EIRNA Bio.