GSM9098522: Sheared,12hr,rep1; Homo sapiens; RNA-Seq
Cytosolic RNA was extracted by lysis in RLN buffer (50mM Tris-HCl pH=8, 140m NaCl, 1.5mM MgCl2, 0.5% NP-40) for 5 minutes, follwed by centrifugation at 12,000g for 5 minutes. Trizol was then added to the supernatant and the Direct-zol RNA Purification Kit (R2050, ZYMO Research) was used according to the manufacturer's guidelines. Sequencing and library contruction was performed by Novogene Co