RNA-Seq of GS115-No DTT
Total RNA was extracted from cells using TRIzol reagent. Ribosomal RNA (rRNA) was removed using Kit. The remaining RNA was fragmented and reverse-transcribed into cDNA, which was then used to construct a strand-specific RNA-seq library by end-repair, dA-tailing, adapter ligation, and PCR amplification. The library was sequenced on the [Illumina  Novaseq 2x150bp] platform with 150 bp paired-end reads.