resequencing of the fin of Larimichthys crocea
Genomic DNA, randomly fragmented into short segments by enzymes, underwent blunt - end repair. Subsequently, dA tails were attached to the ends of these fragments, followed by adapter ligation. The adapter - linked DNA fragments were purified via AMPure XP beads, with fragments in the 300 - 400bp range selected for PCR amplification. The constructed library, after purification and quality checking, was sequenced on the Hiseq X10 PE150 platform.