Pacbio of Spirodela polyrhiza: adult whole plant
Single Molecule Real-Time (SMRT) sequencing libraries were constructed using the SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences), according to the manufacturers instructions. The libraires underwent purification with AMPure PB beads (Pacific Biosciences) and size selection ( 5 kb) using BluePippin (Sage Science), followed by sequencing on two SMRT cells on the Sequel II platform.