Groundwater 16S rRNA, CIZ SET-1, 14 m bgs, 2019-8-19
16S rRNA gene amplicon libraries were prepared from groundwater DNA samples by Welgene Biotech Co., Ltd. (Taipei, Taiwan). The V3V4 hypervariable regions were amplified using primers Pro341F (5-CCTACGGGNBGCASCAG-3) and Pro805R (5-GACTACNVGGGTATCTAATCC-3). Libraries were generated using a two-step PCR protocol with dual-index barcoding and sequenced on the Illumina MiSeq platform. Raw reads were demultiplexed, PhiX reads were removed, and primer sequences were trimmed using the cutadapt plugin before QIIME 2 analysis.