Probe-capture + skim-seq WGS of SHB blueberry TH-3383 (B626)
Genomic DNA from leaf tissue was subjected to hybridization capture using a custom blueberry probe set (Twist Bioscience) targeting ~9.83 Mb across the Suziblue haplotype 1 reference genome. Both on-target (enriched) and off-target reads were retained and combined with low-pass skim-seq WGS data. Libraries were sequenced as 150 bp paired-end reads on the Illumina NovaSeq X.