Y120-C-1 (CRX2025362; CRA030492)
The gut contents of three 5th instar (day 5) silkworms from each group were pooled to form one biological sample, with six replicates prepared for 16S rRNA gene sequencing. Total microbial genomic DNA was isolated using the Soil and Stool DNA Extraction Kit (TIANGEN Biotech, DP712). The hypervariable V3_V4 region of the bacterial 16S rRNA gene was amplified using the primer pair 341F/805R with TaKaRa Ex Taq polymerase. Purified amplicons were then subjected to paired-end sequencing on the Illumina MiSeq PE250 platform.