GSM9843385: Human PBMC PCOS GEX; Homo sapiens; RNA-Seq
Peripheral blood mononuclear cells were isolated from peripheral blood using density gradient centrifugation. Single-cell suspensions were prepared, filtered, and assessed for cell viability and concentration before single-cell library preparation. Single-cell gene expression libraries were prepared using the 10x Genomics Chromium Single Cell 5' Gene Expression platform. Single-cell TCR V(D)J libraries were prepared from the same cell suspensions using the 10x Genomics Chromium Single Cell V(D)J enrichment workflow according to the manufacturer's instructions.