RNA-seq of T. aestivum cv. CDC Stanley mutant M10 (CRX2018597; CRA030381)
1. Take a certain amount of total RNA samples, and use oligo dT beads to enrich mRNA with poly A tail;2. mRNA molecules were fragmented into small pieces; 3. The fragmented mRNA was synthesized into first strand cDNA using random primers; 4. The second strand cDNA was synthesized; 5. The synthesized cDNA was subjected to end-repair and 3' adenylated. Adaptors were ligated to the ends of these 3' adenylated cDNA fragments; 6. PCR amplification and purification; 7. Library quality control; 8. Library circularization; 9. The library was amplified to make DNA nanoball (DNB); 10. Sequencing on DNBSEQ (DNBSEQ Technology) platform.