L1EIE2003007 (CRX2024018; CRA030459)
"Purified mRNA was fragmented at 94 degC for 5-7 min using divalent cations. cDNA libraries were constructed with an Illumina RNA-seq sample preparation kit (San Diego, CA) following the manufacturer's instructions. Qualified libraries were sequenced on an Illumina NovaSeq 6000 platform, generating 169 bp paired-end reads."