GSM9455389: IgG Control K562 - CUT&RUN; Homo sapiens; OTHER
CUT&RUN was performed with 500K native or lightly fixed (0.1% formaldehyde for one min) digitonin (0.01%) permeabilized cells immobilized on Concanavalin A magnetic beads, 0.5 μg antibody, and overnight incubation at 4ºC. After the addition and activation of pAG-MNase, CUT&RUN-enriched DNA was purified as per kit instructions or using SPRIselect beads and quantified by PicoGreen. Sequencing libraries were prepared with 5 ng DNA (or total recovered if less) and the CUTANA CUT&RUN Library Prep kit. Libraries were analyzed by Agilent TapeStation, pooled to equivalence, and sequenced (Illumina NextSeq 2000), targeting 7-10 million paired-end reads (PE100 unless otherwise stated) per reaction.