GSM9420233: Direct Wild; Mus booduga; RNA-Seq
Total RNA was extracted from the hippocampus using All prep DNA/RNA kit (Cat# 80204; Qiagen). The quantity and quality of RNA were examined using Qubit fluorometer (Cat# Q32855; Thermofisher) and Nanodrop ™1000 respectively. The library preparation was carried out using NEBNext Ultra II Directional RNA library Prep Kit (NEB#E7770). Final libraries were quantified using Qubit 4.0 fluorometer (Thermofisher #Q32855) using DNA HS assay kit (Thermofisher #Q32851) following manufacturer's protocol. Obtained results are presented below in the table. To identify the insert size of the library, we queried it on Tapestation 4150 (Agilent) utilizing high sensitive D1000 screentapes (Agilent #5067- 5582) following manufacturers' protocol.