GSM8888234: 293T DMSO no break rep1 CALRi2; Homo sapiens; OTHER
Cells were trypsinized and genomic DNA was harvested using the Zymo Research Miniprep Kit. 2µg of gDNA was used for library preparation. Locus-specific primers were used to amplify (35 cycle) target sequences of flanking exons. PCR products were subjected to a second round of PCR (11 cycle) with unique indexing sequences (i5 and i7). Samples were run by the MSK IGO core on the Illumina NovaSeq X instrument. Sequencing was performed using a 2x150 paired-end (PE) configuration.