Human pluripotent stem cell-derived kidney organoids reveal tubular epithelial pathobiology of heterozygous HNF1B-associated dysplastic kidney malformations K
Human pluripotent stem cell-derived kidney organoids reveal tubular epithelial pathobiology of heterozygous HNF1B-associated dysplastic kidney malformations K
First trimester fetal kidneys collected after maternal consent and ethical approval (ethics REC 08/H0906/21+5; and REC 18/NE/0290) and were provided by the MRC and Wellcome Trust Human Developmental Biology Resource (http://www.hdbr.org/). Total RNA was extracted using the miRVana miRNA isolation kit (Thermo Fisher, AM1560) without using the optional miRNA-enriching protocol  Truseq stranded mRNA Assay. Paired end.