GSM8851901: 5/6 nephrectomy; Mus musculus; RNA-Seq
The right kidney was exposed through a flank incision and removed. After a week, the left kidney was exposed and the upper and the lower poles were ligated with a 3-0 non-absorbable suture until the coil diameter was half of the kidney. For control animals (sham), each flank of the kidney underwent sham surgery by incising flanks, exposing the kidney, and suturing each flank. Four weeks after surgery, the mice were sacrificed under anesthesia to collect kidney samples. Nuclei were isolated from frozen kidney tissue using a Chromium Nuclei Isolation Kit (10x Genomics), following the manufacturer's protocol. Single nuclei, diluted to a concentration of 1000 nuclei/mL, were captured using the Chromium System (10x Genomics) in accordance with the manufacturer's protocol.