GSM8502604: control BR3; Mus musculus; OTHER
Genomic DNA was isolated from sorted wild type CD4 cells, or sorted expanded CD45.2+ Tet2/3 DKO cells after one or two transplantations  in congenic CD45.1+ recipient mice using the PureLink genomic DNA mini kit (Invitrogen, catalogue number K182001). DNA was quantified (after dilution) using Qubit 1X dsDNA High Sensitivity (HS) (Invitrogen, catalogue number: Q33230) in a Qubit 4 fluorometer (Invitrogen). DNA was provided to the UNC High Throughput Sequencing Facility and libraries were prepared using Illumina PCR free DNA library prep kit. Paired end, 2x150 bp length sequencing was performed using Illumina NextSeq2000 P1 system