Amplified 18S SSR rRNA gene from Antarctic cryoconite sample V06
18S SSU gene was PCR amplified in triplicates from each sample using the 1391f-EukBr primers with attached Illumina barcodes unique to each sample. The amplified DNA samples were pooled and then normalized to equimolar concentrations utilizing SequalPrep Normalization Plate Kits (Invitrogen Corp., Carlsbad, CA, United States). The ensuing amplicons were sequenced, after library preparation, on the Illumina MiSeq platform (2  250 bp chemistry, ~17 hours, MiSeq Reagent Kit v2 Nano) at the BioFrontiers Sequencing Core Facility at the University of Colorado, Boulder.