ONT DNA-Seq of Escherichia coli HS phiHS2-resistant mutant
For long-read sequencing, high-molecular-weight genomic DNA was purified from overnight bacterial cultures using the Monarch Genomic DNA Purification Kit. Libraries were prepared from 500 ng of XbaI-digested DNA using the Native Barcoding Kit 24 V14 (SQK-NBD114-24) and the Ligation Sequencing Kit XL V14 (SQK-LSK114-XL) with long-fragment enrichment using the Long Fragment Buffer according to the manufacturers instructions. Sequencing was performed on a PromethION device using an R10.4.1 flow cell (FLO-PRO114M) with MinKNOW v23.11.2. Basecalling was performed using Dorado v0.5.3 with the super-accurate model.