GSM8567482: Rh30,ETS1; Homo sapiens; OTHER
Cells were harvested, immobilized on Con-A beads, permeabilized with digitonin, and incubated with antibodies overnight. Antibody-bound chromatin was released using pAG-MNase with CaCl2 activation. DNA was purified using NEB Monarch DNA cleanup kit. CUT&RUN libraries were assembled using the NEBNext Ultra II DNA Library Prep Kit for Illumina and dual-index primers, followed by size selection/adaptor cleanup using AMPure XP beads, and size distribution assessment using high-sensitivity Agilent DNA chips.