RNAseq of PG-DhESCs
After the RNA sequencing data were obtained, low-quality reads with quality less than 20 and length less than 36 were removed using TRIMGALORE software. Eligible reads were mapped into the mouse reference genome of GRCm39 with STAR software and the raw counts of gene expression of each sample were obtained with featurecounts. The raw count numbers of genes from different sample  were converted into TPM using a self-written Perl script to compare the differences between groups, and the significance analysis of differences was completed by DEseq3 software.