Long Read sequencing HMW DNA Creinhardtii CC-2937 run 3
HMW DNA extracted with phenol chloroform, sheared 30X with a 27 G syringe needle, and size selected with 0.7x AMPure beads. 3.5 ug of DNA was ligated with the SQK-LSK114 kit and loaded three times in a PromethION flowcell (370 ng/load) and two additional times in reused flowcells.