A152 (CRX2021971; CRA030441)
Microbial genomic DNA was extracted from homogenized fecal matter using the QIAamp Fast DNA Stool Mini Kit (Qiagen, Hilden, Germany), following the manufacturer's guidelines. The hypervariable V3-V4 regions of the 16S rRNA gene were PCR-amplified using primers 341F (CCTACGGGGNGGCWGCAG) and 805R (GACTACHVGGGTATCTAATCC).