DNA-Seq of Escherichia coli HS phiHS1-resistant mutant
Libraries for wholegenome sequencing were prepared from 500ng of DNA using the MGI Easy PCRFree Library Prep Set according to the manufacturer's instructions. Enzymatic fragmentation was performed as recommended, followed by size selection of 400450bp fragments using the provided DNA Clean Beads. Library concentration was measured using a Qubit Flex fluorometer with the dsDNA HS Assay Kit. Library quality was assessed with a 4200 TapeStation System using the High Sensitivity D1000 ScreenTape Assay. Libraries were circularized, pooled, and sequenced on a DNBSEQG400 platform in 2150bp pairedend mode.