16S of L1
Total genomic DNA was extracted from group lactate sampled on day 80 using the PowerSoil DNA Isolation Kit (Qiagen). The quality and quantity of the DNA were determined using a NanoDrop 2000 spectrophotometer (Thermo Scientific). The 16S rRNA gene from 7 samples was amplified using Oxford Nanopores 16S Barcoding Kit 1-24, with universal primers 27F and 1492R, followed by adaptor ligation using the NEBNext Quick Ligation Module.