WGS of Aspergillus fumigatus: Lung transplant BAL
Genomic DNA was extracted from subcultured isolates grown on Sabouraud Dextrose Agar at 37C for 3 days. Conidial suspensions were lysed using the MasterPure Yeast DNA Purification Kit with bead beating (1.0 mm zirconium oxide beads) and further processed with the Qiagen DNeasy Blood & Tissue Kit, followed by RNAse and proteinase K treatment. DNA was cleaned using the Zymo Genomic DNA Clean and Concentrator Kit and quantified using a Qubit fluorometer. Samples were diluted to 20 ng/L and submitted for whole genome sequencing. Library preparation was performed using the Illumina TruSeq Nano DNA Library Prep Kit with size selection and limited-cycle PCR. Sequencing was conducted on an Illumina HiSeq 2500 platform (150 bp paired-end, high-output mode).