GSM9664545: CNL_LDR_UT_rep1; Mus musculus; RNA-Seq
RNA was extracted from isolated splenocytes using Trizol (Thermofisher). RNA integrity was ensured with a minimum RIN cut-off of 6.5. 6 RNA samples per radiation condition were submitted for sequencing. 3 Samples (A3, B2, and C1) did not pass quality control. Samples with proper quality metrics proceeded. Poly-A enriched stranded cDNA libraries were generated and subjected to 100 bp paired-end sequencing on a NovaSeq6000 at Genome Quebec (Montreal, Canada).