GSM9127558: NC_3; Homo sapiens; RNA-Seq
Total RNA was isolated and purified using TRIzol(Life, cat.265709, CA, USA) following the manufacturer's procedure. VAHTS mRNA Capture Beads with Oligo (dT) (Vazyme, cat.N401-01, Nanjing, China) were used and mRNA was fragmented using VAHTS Universal V6 RNA-seq Library Prep Kit (Vazyme, cat.NR604, Nanjing, China) under 94°C for 8 min, followed by reverse transcription into cDNA, which was used to synthesize U-labeled second-strand DNA.