KO1
Strand-specific cDNA libraries were prepared using Illumina TruSeq Stranded mRNA Library Prep Kit. Polyadenylated transcripts were captured from total RNA using oligo(dT) magnetic beads, fragmented, and reverse transcribed using random primers with a dUTP incorporation strategy for second-strand marking. The resulting cDNA was end-repaired, A-tailed, and ligated with Illumina adapters. Libraries were amplified by PCR and size-selected to an average insert size of ~303 bp