TCR Amplicon of human: healthy donor CD3+ T cells
TIRTL-seq: Healthy donor CD3+ T cells sorted as single cell per well, lysed and mRNA reverse transcribed using poly-A and random hexamers in Maxima-H RT reaction. cDNA amplified with TCR V segment and C segment multiplex primers in PCR1 which was then amplified with well specific dual-index primers containing Illumina adapters in PCR2. Libraries from other experiment (RT with CellDirect) made using unique pairs of TCR-alpha and TCR-beta C-segment primers with inline barcodes were pooled for sequencing. This is demultiplexed data for the TIRTL-seq RT condition with abTCR-oldID02 barcode.