PacBio of 103 sample
Purification of the amplified products was performed using the QIAquick Gel Extraction Kit (Qiagen, Santa Clarita, CA, USA). Equimolar concentrations of the purified DNA from all samples were pooled to prepare sequencing libraries. Library construction utilized the SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences, Menlo Park, CA, USA), following the manufacturer's guidelines as described in the protocol for Full-Length 16S and 18S Gene Amplification with Barcoded Primers for Multiplexed SMRTbell Library Preparation and Sequencing. Sequencing was conducted on the PacBio Sequel II platform using the Sequel II Sequencing Kit 2.0 (PacBio, USA).