Sequenced wild-fermented cider microbiota
DNA libraries were prepared using the Watchmaker DNA Library Prep Kit. Genomic DNA was fragmented using a mastermix of Watchmaker Frag/AT Buffer and Frag/AT Enzyme Mix. IDT xGen UDI Primers and IDT Stubby Adapters were added to each sample followed by 7 cycles of PCR to construct the DNA libraries.  The final DNA libraries were purified using AMPure magnetic beads (Beckmman Coulter) and eluted in nuclease-free water. Following elution, the libraries were quantified using the Qubit fluorometer dsDNA HS Assay Kit. Libraries were then sequenced on the Element AVITI platform 2x150 bp.