GSM9229483: RNA-Seq, PDX-2, PBS; Homo sapiens; RNA-Seq
A moderate amount of tumor tissue was placed in ISOGEN (NIPPON GENE), crushed with a mixer, and RNA was extracted according to ISOGEN protocol. Ribosomal RNA was depleted with the Ribo-Zero Plus rRNA Depletion Kit (Illumina). Stranded libraries were prepared using the TruSeq Stranded mRNA Sample Preparation Kit (Illumina) following the manufacturer's protocol, omitting the mRNA purification step.