GSM9698002: SK-N-BE(2), N‑CTAD, rep2; Homo sapiens; RNA-Seq
RNA was extracted using the Qiagen RNeasy Mini Kit according to manufacturer's instructions. Total RNA (~250 ng input) was used for library preparation with the Illumina Stranded mRNA Prep kit (poly(A) selection and fragmentation), followed by first- and second-strand cDNA synthesis, end repair, A-tailing, and adapter ligation. Libraries were amplified using 13 PCR cycles and indexed with unique dual indexes. Library quality and concentration were assessed using TapeStation (Agilent) and Qubit fluorometry (Thermo Fisher Scientific).