GSM9456143: l02-1 [long RNA]; Homo sapiens; RNA-Seq
The protocol involved the extraction of total RNA from cultured L02 and Hep3B cell lines using TRIzol Reagent, followed by quality control of the RNA using a Bioanalyzer and NanoDrop spectrophotometer. Subsequently, sequencing libraries were constructed from the qualified RNA with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina according to the manufacturer's instructions, which included mRNA enrichment, fragmentation, cDNA synthesis, and adapter ligation. The final prepared libraries were then sequenced on an Illumina NovaSeq 6000 platform to generate paired-end reads. RNA libraries were prepared for sequencing using standard Illumina protocols