GSM8988987: mNPC cells , BCOR-ITD2, Day0, rep3; Mus musculus; RNA-Seq
Total RNA was isolated from 1x106 cells mES cells or mNPC derived from mES cells using the RNeasy Plus Mini Kit (Qiagen, 74134). mRNA was selected using PolyA enrichment (New England Biolabs, E7490S). RNA quality was analyzed using RNA screen tape (Agilent Technologies, 5067-5576) on an Agilent Tape Station. Sequencing libraries were generated as per manufacturer protocol using the NEBNext Ultra II Directional RNA Library Preparation Kit (New England Biolabs, E7760). Library concentration and fragment sizes were analyzed with the D1000 Screen tape (Agilent Technologies, 5067-5582). Libraries were pooled and sequenced on the Illumina NovaSeq 6000 using the Harvard Medical School Biopolymers Facility.