RNA-seq from the prosencephalon of 12-day-old embryo from control and diabetic rats
RNA-seq from the prosencephalon of 12-day-old embryo from control and diabetic rats
At embryo day 12, pregnant rats were euthanized by decapitation following anesthesia induction with sevoflurane; embryos were collected by cesarian and washed in cold phosphate-buffered saline (PBS, pH 7.4). The hole prosencephalon of embryos without neural tube defects was directed and placed in Trizol for RNA extraction. Embryos of both sexes were used without distinction in all experiments.  Total RNA was obtained using TRIZOL® reagent (Thermo Fisher Scientific, MA, USA) following provider's instructions. RNA quality and purity were determined (Nanodrop, Thermo Fisher Scientific), and only 260/230 ≥ 1.0 and 260/280 ≥ 1.8 samples were used. RNA Integrity Number (RIN) was obtained by using a BioAnalyzer (Agilent 2100 BioAnalyzer, CA, USA). The mean RIN was 9.04 ± 0.44 RNA-seq libraries were prepared using the Truseq Stranded mRNA library prep kit from (Illumina, CA, USA) according to the manufacturer's instructions. 