Transcriptome Sequencing of HTR-8: adult female placenta
After total RNA was extracted from the sample, mRNA was enriched with magnetic beads with Oligo (dT). A fragmentation Buffer was added to the obtained mRNA to make the fragments into short fragments. The first cDNA strand was synthesized using random hexamers based on the fragment mRNA as a template. The second strand of cDNA was synthesized by adding buffer, dNTPs, RNase H and DNA polymerase I, which was purified and eluted with EB buffer solution through terminal repair, base A and sequencing joint, then recovered by agarose gel electrophoresis and amplified by PCR to complete the entire library preparation. The constructed library was sequenced by Illumina HiSeq2000. The experiment was commissioned by Sangon Biotech Co., Ltd.(China).