Bisulfite amplicon sequencing of the 3_ junction between an R2Tg retrotransposon mediated mCherry insertion and the 25S rDNA locus in Arabidopsis thaliana.
Genomic DNA was treated with bisulfite to convert cytosine residues to uracil depending on methylation status prior to PCR and amplicon sequencing to assess methylation status of the insert 3' end and downstream rDNA.