GSM9422182: iron_deficiency_anemia_tooth; Rattus norvegicus; OTHER
10 μm tissue sections were mounted onto Stereo-seq chips (1 cm2 size, generated by BGI, China). which feature capture spots with 220 nm diameter and 500 nm spacing. After fixation (pre-chilled methanol) and staining (Qubit ssDNA dye, Thermo Fisher, USA), tissues were permeabilized using 0.1% pepsin (Sigma, USA) at 37℃. In situ RNA was reverse transcribed into cDNA using the capture probes (containing 10 bp MID and 25 bp CID barcodes). The cDNA was released, amplified using cDNA HIFI PCR mix (MGI), and fragmented (400-600 bp) Stereo-seq transcriptomic library preparation and sequencing were performed following the protocol previously described by 10.1016/j.cell.2022.04.003