FD2 (CRX2014344; CRA030238)
The qualified DNA samples were randomly sheared into fragments of approximately 351 bp using a Covaris disruptor. Library construction was performed using the TruSeq Library Construction Kit, strictly following the manufacturer's protocols for recommended reagents and consumables. The entire library preparation process involved end repair, poly-A tail addition, sequencing adapter ligation, purification, and PCR amplification.