GSM8709699: S.Tm_wt_infected_1; Homo sapiens; RNA-Seq
Total RNA was extracted with the RNeasy Micro Kit (Qiagen, #74004) and stored at -80 °C until submission. Sequencing libraries were prepared from 500ng total RNA using the TruSeq stranded mRNA library preparation kit (cat# 20020595, Illumina Inc.) including polyA selection. Unique dual indexes (cat# 20040871, Illumina Inc.) were used. The library preparation was performed according to the manufacturers' protocol (#1000000040498).