RNA-seq; comparison of root and shoot transcriptome between WT and strigolactone-insensitive mutant (hvd14.d), barley in response to cadmium (Cd) and zinc (Zn) treatment; three-week-old seedlings
RNA-seq; comparison of root and shoot transcriptome between WT and strigolactone-insensitive mutant (hvd14.d), barley in response to cadmium (Cd) and zinc (Zn) treatment; three-week-old seedlings
For RNA-isolation analyses, plant tissue (shoot and root) was collected in four biological replicates, each containing tissue from 4 seedlings.  For the RNAseq experiment, plants were grown in hydroponic conditions up to 21 days. Six plants were placed in the 1.5 l container filled with ½ Hoagland solution (Hoagland and Dennis, 1938). The medium was replaced every week. Plants were placed in the greenhouse under a 20/18°C day/night, 16/8 photoperiod and 420 μE m−2 s−1 light intensity. After eleven days of growth in control conditions, the containers were divided into variants: control, treated with Cd at a concentration of 5 µM, or treated with Zn at a concentration of 50 µM. The treatment lasted another ten days.  Samples were frozen immediately in liquid nitrogen; RNA was isolated using the mirVana miRNA Isolation Kit (Sigma, catalogue number: AM1560).  Preparation of RNA library was conducted by Novogene Co., LTD (Beijing, China). 