RNAseq of primary fibroblasts from people living with and without HIV
Primary dermal fibroblast cultures were established from 6 mm skin punch biopsies obtained from six virologically suppressed people living with HIV-1 (PLWH) and seven age- and sex-matched people without HIV-1 (PWOH). Biopsy tissue was dissected into small fragments and cultured on 0.1 % gelatin-coated plates in DMEM high glucose supplemented with 10 % fetal bovine serum, 1 NEAA, and 1 L-glutamine at 37 C and 5 % CO until fibroblast outgrowth was observed. Fibroblasts were expanded by routine passaging with trypsin-EDTA. Control fibroblast lines from additional donors were obtained from the Coriell Institute or NIA Aging Cell Culture Repository.