GSM9455724: CAL27 cells, Control, 24h, replicate 2; Homo sapiens; RNA-Seq
Total RNA was isolated using the RNeasy Mini Kit from HNSCC cell lines (CAL27 or SCC25), and 500 ng of total RNA were used for the construction of the sequencing libraries. Sequencing libraries were prepared using (i) NEBNext Poly(A) mRNA Magnetic Isolation Module and sequencing library was prepared with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina using NEBNext Multiplex Oligos for Illumina and Agencourt Ampure XP Beads for cell line samples and (ii) QuantSeq 3′ mRNA-Seq V2 Library Prep Kit FWD with UDI 12 nt in combination with UMI Second Strand Synthesis Module for QuantSeq FWD.