RNA seq of Pseudomonas aeruginosa WD4
The QIAseq Stranded mRNA Library Kit was employed to enrich mRNA and generate strand-specific Next-generation sequencing (NGS) libraries. The QIAseq Stranded mRNA Library Kits were used to produce NGS libraries for sequencing on Illumina NGS systems, namely the NovaSeq 6000. Unique dual indexes are used for 74 bp paired-end reads and dual 10 bp index reads. Combinatorial indexes are used for 76 base pair paired-end reads and dual 8 base pair index reads. The library quality evaluation was conducted utilizing the Agilent D5000 ScreenTape System and Agilent D1000 ScreenTape System in the 4150 Tape Station System, all manufactured by Agilent. This system is specifically developed for the analysis of DNA molecules ranging in size from 100 to 5000 base pairs. 1 microliter (L) of the purified library was combined with 10 microliters (L) of D5000 sample buffer and mixed vigorously using an IKA vortex mixer at a speed of 2000 rpm for 1 minute. The mixture was then centrifuged to collect the sample at the bottom of the strip. Subsequently, the strip was inserted into the Agilent 4150 TapeStation system