GSM9098385: White, Replicate 2, sRNAseq; Dahlia pinnata; miRNA-Seq
Total RNA was extracted from the dissected dahlia petals, namely the pigmented (red) and unpigmented (white) regions of bicolor petals, using Trizol reagent according to the manufacturer's protocol (Thermo Fisher Scientific). The small RNA-sequencing library was prepared using a NEBNext Small RNA Library Prep kit (New England Biolabs Japan, Tokyo, Japan) according to the manufacturer's instructions.