ST4 wild type RNA-seq
Bacterial cells were grown in PDB medium in the absence of haploid sporidia of Sporisorium scitamineum, MAT-1 and MAT-2 cells,  and harvested at OD600 of 1.0 by centrifugation. The Eastep super total RNA extraction kit (Promega) was used for RNA purification. After electrophoretic quality control, the RNA samples were sent to Gene Denovo (China) for transcriptome sequencing.