GSM9128024: HUVEC + 2.5 µM THC; Homo sapiens; RNA-Seq
Total RNA was extracted from HUVECs using TRIzol reagent according to the manufacturer's instructions. Poly(A)-selected mRNA was fragmented and used for cDNA synthesis with ProtoScript II. Second-strand synthesis followed, then end repair, A-tailing, adapter ligation, and size selection (~420 bp) via magnetic beads. Libraries were amplified (13 PCR cycles), quantified by Qubit 3.0, and sequenced using the Illumina HiSeq 4000 platform.