GSM9196448: LO, SR5, 72hr, rep3; Euprymna scolopes; RNA-Seq
Total light-organ RNA was isolated using an RNeasy Mini Kit (Qiagen, Germantown, MD, #74106) and Qiagen QIAshredder according to the manufacturer's instructions. Samples were treated with TURBO DNase (Thermo Fisher Scientific, #AM1907) to remove DNA contamination. mRNA was isolated from total RNA using NEBNect Poly(A) mRNA Magnetic Isolation Module (NEB, #E7490), and RNA-seq libraries were constructed using the NEBNext Ultra II RNA Library Prep Kit for Ilumina (NEB, #E7770) following the manufacturer's protocols