GSM9634104: AEAA mid-dose lung biol rep 3; Mus musculus; RNA-Seq
Total RNA was extracted from lung tissues using TRIzol reagent according to the manufacturer's instructions. RNA integrity and quality were assessed using an Agilent 5300 Fragment Analyzer, and RNA concentration was measured using a NanoDrop ND-2000 spectrophotometer. Total RNA was extracted from frozen lung tissues using TRIzol reagent. RNA quantity and purity were assessed using a NanoDrop 2000 spectrophotometer, and RNA integrity was evaluated using an Agilent 5300 Fragment Analyzer. Samples with RIN ≥ 7.0 and OD260/280 ratios between 1.8 and 2.1 were used for library preparation. mRNA was enriched by removing rRNA, followed by fragmentation, first-strand and second-strand cDNA synthesis, adapter ligation, and PCR amplification. After library quality control, the libraries were sequenced on an Illumina NovaSeq 6000 platform in paired-end 150 bp mode (PE150).