Pi16h_3 (CRX2017481; CRA030347)
Total RNA underwent quality control, followed by mRNA isolation using oligo(dT) beads and fragmentation. Double-stranded cDNA was synthesized, end-repaired, A-tailed, and adapter-ligated. The library was PCR-amplified, quality-checked, and circularized. Sequencing was performed on a patterned nanoarray using combinatorial Probe-Anchor Synthesis (cPAS) technology following rolling circle amplification into DNA nanoballs. submitted_instrument_model: MGISEQ-2000