RNAseq-KO1 (CRX825613; CRA012916)
Total RNAs from peritoneal macrophages isolated from LPS-stimulated Pcif1 KO mice and WT littermates were extracted by Trizol reagent (Invitrogen) and purified by using poly-T oligo-attached magnetic beads. Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB) following manufacturer's protocols, and were then subjected to Illumina NovaSeq 6000.