WGS of Escherichia coli BW25113: co-evolved population under medium osmolality
Whole-genome sequening was performed on bacterial populations from a bacteria-phage co-evolution experiment in different osmotic conditions. Genomic DNA was extracted from frozen glycerol stocks without regrowth to minimise further evolutionary changes. DNA was then extracted using the DNeasy Blood and Tissue Kit (Qiagen) according to the manufacturer's instructions. Library preparation and sequencing were performed by the Sequencing and Bioinformatics Consortium at The University of British Columbia. Extracted DNA was quantified using Qubit fluorometry. Genomic libraries were prepared using the Illumina DNA Prep kit (Illumina). Samples were sequenced with the Illumina NextSeq 2000 sequencing platform generating paired end 150 bp reads.