GSM9664327: 28_rgal; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNeasy Mini Kit (Qiagen, Germany) with DNase treatment following the manufacturer's protocol. RNA integrity was verified using an Agilent Bioanalyzer or 4200 TapeStation. Libraries were generated from 1-50ng of total RNA. ds-cDNA was synthesized using the SeqPlex RNA Amplification Kit (Sigma-Aldrich). cDNA was fragmented using a Covaris E220 sonicator (peak incident power 18, duty factor 20%, 50 cycles/burst, 120s), followed by blunt-ending, 3' A-tailing, and Illumina adapter ligation. Ligated fragments were PCR-amplified for 12-15 cycles using unique index primers.