HepG2 cell
Total RNA was extracted from HepG2 cells for whole-transcriptome sequencing. Poly(A)+ mRNA was enriched using oligo(dT) magnetic beads and fragmented. Double-stranded cDNA libraries were constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina. Libraries were quality assessed using an Agilent Bioanalyzer 2100 and sequenced on the Illumina NovaSeq 6000 platform to generate paired-end 150 bp reads.