GSM9844589: PC9-Par, rep2; Homo sapiens; RNA-Seq
RNA was extracted using TRIzol Reagent. RNA integrity was assessed using a Bioanalyzer 2100. Genomic DNA was eliminated through two rounds of digestion using amplification-grade DNase 1. The processed RNA was fragmented and subjected to reverse transcription using random primers to generate cDNA, which was utilized for library preparation. Library quality was assessed using the Bioanalyzer 2100.