ChIP-seq to identify binding sites of the fusion oncogene CBFA2T3::GLIS2 in WSU-AML cell line
ChIP-seq to identify binding sites of the fusion oncogene CBFA2T3::GLIS2 in WSU-AML cell line
For each replicate, 2M cells were fixed in 1% formaldehyde and reaction was stopped with 125mM Glycine. After cell lysis, chromatin was digested by Micrococcal nuclease(NEB) and sonicated during 15s with Bioruptor Plus (Diagenode) at medium intensity. Sheared chromatin was then used for immunoprecipitation. DNA fragments were amplified by PCR with indexed paired-end adaptors (Illumina).