RNA-Seq of Nicotiana tabacum: Y99 0h rep2
Transcriptome sequencing was performed to investigate the cold tolerance mechanisms in two tobacco varieties (YM2020 and Y99). Seedlings were subjected to cold stress (4C) for 0, 2 hours. Total RNA was extracted from leaves and mRNA was purified using poly-T oligo-attached magnetic beads. Sequencing libraries were constructed using standard Illumina protocols and sequenced on the Illumina NovaSeq 6000 platform with paired-end 150 bp reads.