GSM8887030: R2, Recurrent 2; Homo sapiens; RNA-Seq
RNA was purified from FFPE tissues with the Quick-DNA/RNA FFPE kit (R1009, Zymo Research). The concentration of RNA was assessed with a Nanodrop spectrophotometer. A 20 uL volume of at least 400 ng RNA was used for library creation. Libraries were generated with the SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian (Takara Bio USA)