GSM9421199: OT-II_IL-2(WT); Mus musculus; RNA-Seq
One million sorted Thy1.1⁺ naïve OT-II cells were adoptively transferred into Thy1.2⁺ Foxp3-GFP recipient mice via retro-orbital intravenous injection. This was followed by six intraperitoneal injections of 100 μg OVA protein combined with 50 pmol of the indicated proteins, administered every other day. On day 11, donor OT-II cells and host Foxp3-GFP⁺ CD4⁺ T cells were sorted from the mLNs of the respective treatment groups and submitted to MedGenome, Inc. for library preparation and RNA sequencing. The library was constructed using 10X Genomics Single Cell 3' v4 (polyA) chemistry according to the manufacturer's protocol.