Effective requesting method to detect fusion transcripts in chronic myelomonocytic leukemia RNA-seq
Effective requesting method to detect fusion transcripts in chronic myelomonocytic leukemia RNA-seq
10 samples from Bone marrow or peripheral blood separated on Fycoll-Hypaque. CD14+ monocytes were then sorted from PBMCs with magnetic beads and the AutoMacs system as previously described by Merlevede et al. (2016) Total RNA was isolated using mini RNeasy kit (Qiagen) according to the manufacturer instructions. The quantity and quality of extracted RNA was evaluated using the NanoDrop ND-1000 spectrophotometer and the Agilent 2100 BioAnalyzer.  Libraries have been prepared using the “TruSeq Stranded Total RNA Gold” Kit from Illumina.