HMW
Culture grown at 16 C for 7 days in liquid PD and it was carefully carried out using the CTAB method (Neuhauser et al 2009) with few modifications. First, culture material was washed with sterile water and rupture of the mycelium was done by 1 x cycle of 30s of using Precellys Evolution homogenizer with micro glass beads (Precellys Lysing Kit CK28). For High Molecular Weight DNA  isolation Monarch HMW DNA Extraction Kit for Cells & Blood [NEB] was used. All pipetting was conducted as gently as possible. Vortexing was avoided and substituted with gentle flicking of the solutions