cMyc_Hras2 (CRX2017502; CRA030350)
We employ standardized extraction protocols to isolate RNA from tissues or cells, followed by rigorous quality control of RNA samples. Quality assessment is primarily performed using the Agilent 2100 Bioanalyzer to precisely evaluate RNA integrity. mRNA is enriched from total RNA using Oligo dT magnetic beads. After fragmentation, first-strand cDNA is synthesized with random hexamer primers, followed by second-strand cDNA synthesis. Through steps including end repair, poly-A tailing, adapter ligation, size selection, amplification, and purification, the library preparation is completed. submitted_instrument_model: Illumina NovaSeq 5000