GSM9420621: Cortex_Illumina_RNAseq_rep1; Mus musculus; RNA-Seq
Mice were deeply anaesthetised and transcardially perfused with ice-cold artificial cerebrospinal fluid prior to tissue collection. Cerebral cortex and dorsal root ganglia were rapidly dissected, snap-frozen, and stored at −80 °C. Total RNA was extracted using TRI Reagent followed by purification with the Direct-zol™ RNA Microprep Kit, including on-column DNase I treatment. For Oxford Nanopore sequencing, direct RNA libraries were prepared using the Oxford Nanopore Direct RNA Sequencing Kit (SQK-RNA004), with Induro Reverse Transcriptase and SUPERase·In added during library preparation, and sequenced on MinION or PromethION instruments. For Illumina sequencing, strand-specific poly(A)+ RNA libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit and sequenced as 150-bp paired-end reads on an Illumina NovaSeq 6000.