GSM9664525: small cell lung cancer tissue, treated with OSI906 and anti-PD1; Mus musculus; RNA-Seq
Tumor tissues were dissected into 5 mm3 pieces and incubated in a digestion buffer containing 1.0 mg/ml collagenase I (Gibco, 17100–017) and 0.5 mg/ml collagenase IV (Gibco, 17104–019) in DMEM/F12 (Gibco, C11330500BT). The tissues were mechanically pipetted at 10-minute intervals and gently agitated at 37°C for 30 minutes. Subsequently, the resulting mixture was filtered through 100 µm cell strainers. Following red blood cell lysis with ammonium-chloride-potassium (ACK) lysis buffer, cells were collected for subsequent single-cell RNA-seq library preparation. Single-cell RNA libraries were constructed using the Chromium Single Cell 3′ Reagent Kits v3 according to the manufacturer's instructions (10x Genomics).