GSM9421587: Neonatal sample small; Mus musculus; RNA-Seq
Cells were captured using the Chromium X system and Next GEM Single Cell 3' Reagent Kits v3. (10x Genomics), per manufacturer's protocol, yielding ~5,800 and ~18,000 cells from the small and large neonatal islet samples respectively. Final sequencing libraries were assessed using the Agilent High Sensitivity DNA Chip and quantified with the Qubit High Sensitivity DNA Assay Kit (Thermo Fisher). Libraries were sequenced on an Illumina NovaSeq 6000 platform with the S4 Reagent Kit v1.5 (Illumina) at TGen, using a paired end read sequencing of 28 cycles for Read 1, 101 cycles for Read 2, and 10 cycles each for i7 and i5 index reads. Image analysis was performed with Real-time analysis (RTA) v3.4.4 software.