RNA-Seq of skin tissue: Dorsal skin of adult rats.
The experiment was carried out on the Illumina Novaseq 6000 sequencing platform by Shanghai Majorbio Bio-pharm Technology Co., Ltd. The main steps include: (1) Enrichment of mRNA using Oligo dT; (2) Fragmentation of mRNA; (3) Reverse transcription to synthesize cDNA; (4) Ligation of adaptors; (5) Fragment screening and library enrichment; (6) Sequencing.