GSM9229085: GSC23 sh2 rep1; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol Reagent (Life Technologies, 15596018) following the manufacturer's standard protocol. mRNA was isolated from 1 µg of total RNA using mRNA Capture Beads and subsequently fragmented to a size range of 200–500 bp. cDNA synthesis and adaptor ligation were performed using the NEBNext Ultra RNA Library Prep Kit for Illumina. The resulting RNA-Seq libraries were sequenced using 150 bp paired-end reads on a NovaSeq X plus platform.