GSM8677730: IP_Z22: 5-AZA-CdR + ADAR1i-124; Mus musculus; RIP-Seq
RNA was harvested using TRIzol reagent (Invitrogen). At least 1.5 ug of total RNA was used for RNA-seq. For dsRNA immunoprecipitation, J2 anti-dsRNA antibody (Scicons, 10010500) and anti-Z-RNA antibody (Z22)(Absolute Antibody, Ab00783-3.3) were used to enrich the dsRNA species with IgG as control. Input  RNA was converted into Illumina sequencing libraries using the Illumina Standard Total RNA Prep, Ribo-Zero Plus Ligation (Illumina, 20040525), following the manufacturer's instructions with the following modifications. Input RNA, along with Illumina RNA UD Indexes Set A Ligation (Illumina, 20091655), were ligated to the resulting cDNA after second-strand synthesis and end-prepping.