GSM9556713: CAF30 Input; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes at room temperature. Lysates were clarified from sonicated nuclei and chromatins were isolated with antibodies against H3K4me1 and H3K4me3. Sequencing libraries were prepared from 5 ng of DNA using the GenNext NGS Library Prep Kit (Toyobo, LPK-101). These libraries were sequenced using an Illumina NextSeq2000 to a final sequencing depth of 10 to 30 million reads per sample.