RNA-seq of 4T1
otal RNAs were extracted using TRIzol reagent. Next, 2 g total RNAs were used for stranded RNA sequencing library preparation using Ribo-off rRNA Depletion Kit (Human/Mouse/Rat)  and KC-DigitalTM Stranded mRNA Library Prep Kit for Illumina.The kit eliminates duplication bias in PCR and sequencing steps, by using unique molecular identifier (UMI) of 8 random bases to label the pre-amplified cDNA molecules. The library products corresponding to 200-500 bps were enriched, quantified and finally sequenced on DNBSEQ-T7 sequencer with PE150 model.