Fecal metabarcoding of warbler fecal samples
We generated multiplexed dual-index cytochrome oxidase C subunit 1 (COI) amplicon libraries for Illumina MiSeq sequencing. Prior to PCR, we randomized the plate order of samples to avoid within-plate batch effects during amplification. We used the ANML general arthropod primer pair (LCO1-1490/CO1-CFMRa) described in Jusino et al (2019).  We cleaned the indexed PCR product using a double-sided Serapure bead procedure.