transcriptomic analysis of A. fumigatus without or with ETD151 (0.156 µM) or amphotercin B (1.1µM)
transcriptomic analysis of A. fumigatus without or with ETD151 (0.156 µM) or amphotercin B (1.1µM)
Aspergillus fumigatus Af293,in vitro culture (YPD medium) A; fumigatus was incubated for 15 hours at 37°C under agitation at 100 rpm  0, 30 min, 1 hour, 6 hours of either amphotericin B 1.1 µM or ETD151 0.156 µM mechanical lysis ( Magna lysis tubes) : 3 rounds of 3 min at 50 oscillations/min with 10 min of freezing at -80°c between each rounds, repeated twice. Nucleospin miRNA kit (Macherey Nagel®) Librairies were prepared using the Truseq Stranded mRNA Prep protocol from Illumina, according to manufacturer's instructions, starting from 225ng of total RNA.