GSM9227918: Col-0, D8, cauline, LD, EM-seq; Arabidopsis thaliana; OTHER
DNA was isolated using the CTAB method. EM-seq libraries were constructed following the NEBNext® Enzymatic Methyl-seq Kit protocol with 50 ng initial DNA (25 ng for all embryo samples). Library quality was verified with a KAPA Library Quant Kit and fragment analyzer. Libraries were pooled and sequenced using an Illumina NextSeq 2000 (150 bp, paired-end reads) or an NovaSeq X (150 bp, paired-end reads).