ONT of wGF 2-18(ONT)
ONT sequencing is a single-molecule real-time sequencing technology based on nanopore electrical signals. Under the action of motor proteins, the double-stranded DNA binds to the octamer nanopore protein anchored on the biological membrane and unwinds [1]. Due to the potential difference on both sides of the biological membrane, the unwound single-stranded DNA passes through the nanopore at a certain rate. Because of their different chemical properties, different bases cause different changes in electrical signals when passing through the nanopore [2]. By detecting and analyzing these electrical signal changes, the sequence is determined in real time [3].