GSM9046243: embryo E7.5 rep1; Mus musculus; OTHER
Stereo-seq Briefly, following cryosection, tissue sections are mounted on Stereo-seq chips, incubated at 37°C, and fixed with 20°C methanol. The chips underwent ssDNA staining and imaging with a Nikon Ti-7 Eclipse microscope. Tissue permeabilization was performed with 0.1% pepsin in HCl buffer, followed by washing with SSC buffer containing RNAase Inhibitor. Captured mRNA was reverse transcribed at 42°C. Post in situ reverse transcription, the chips were treated with tissue removal buffer and then washed. cDNA was released from the chips using a cDNA Release Mix at 55°C, followed by fragmentation, amplification, and purification to produce a DNBs library.