EGFR L858R+E709K
Sequencing was performed on DNA extracted from FFPE tumor samples. Extracted DNA was fragmented, adapter-ligated, and subjected to solution-phase hybridization with  probes designed to enrich selected gene regions. Enriched  library fragments were sequenced (2  151 base-paired end) using  sequencing-by-synthesis chemistry and the Illumina Miniseq sequencer.