GSM9666134: SY5Y, 40d, 5 uM Mn Replicate 1; Homo sapiens; RNA-Seq
By the end of the exposure, cells were collected with lysis buffer from Invitrogen PureLink RNA mini kit (Invitrogen, Cat. #: 12183018 A), processed with QIAshredder mini spin column (QIAGEN, Cat. #: 175028900), and RNA isolated with the PureLink RNA mini kit following manufacturer's instructions. mRNA sequencing library was prepared using Tecan Universal Plus mRNA sequencing library preparation kit (Tecan, Cat. #: 5020) following manufacturer's instructions.