GSM9128021: Keloid fibroblasts，KF medium, FR-1, rep1; Homo sapiens; RNA-Seq
Bulk RNA-seq: Total RNA was used as input material for RNA sample preparations. RNA quality was verified by spectrophotometry (NanoDrop) and integrity analysis. Polyadenylated mRNA was enriched via poly-A selection, the obtained mRNA was randomly interrupted using divalent cations in the NEB Fragmentation Buffer, followed by library preparation Sequencing libraries were quantified (Qubit), assessed for fragment size distribution (Bioanalyzer), and sequenced on an Illumina platform (NovaSeq) to generate paired-end reads.