H43-27F-1492 16S rRNA amplicon sequencing
After extracting total DNA from the samples, specific primers with barcodes were synthesized based on full-length primers. PCR amplification was performed, and the products were purified, quantified, and normalized to form a sequencing library (SMRT Bell). The constructed library was first subjected to library quality inspection. Qualified libraries were sequenced using the PacBio Sequel platform. The raw data from PacBio Sequel is in BAM format. CCS files were exported using SMRT Link analysis software, and data from different samples were identified according to barcode sequences and converted into FASTQ format data.