GMNL-74 sequencing by Nanopore
In general, the library preparation was followed ONT protocols (Rapid Barcoding Sequencing, SQK-RBK004). 400 ng of genomic DNA was fragmented by transposome randomly, and added barcode in the same time. DNA subsequently purified using AMPureXP beads, and then ligated motor protein-complexed adapter to DNA ends. The library was quantified by Qubit fluorometer, all of final prepared library was loaded onto a running buffer-primed flow cell for sequencing. Sequencing of the native genomic DNA was performed on a single R9.4/FLO-MIN106 flow cell on a GridION.