Lactiplantibacillus plantarum MSR3 whole genome shotgun sequencing project
Genome sequencing of the isolated bacteria was conducted at the Genomic Centre, icddrb,Mohakhali, Dhaka, Bangladesh. The genomic DNA was extracted using the Qiagen DNeasy Blood& Tissue Mini Kit (250) and quality was evaluated using Qubit measurements and Nanodrop forwhole genome sequencing (WGS). Following centrifugation at 14000 rpm for 5 minutes bacterialpellet was discarded and DNA was purified following a modified version of the CDC PULSENETTotal DNA Extraction protocol, employing the Qiagen DNeasy Blood & Tissue Kit. The qualityof the purified DNA was assessed by a Nanodrop spectrophotometer and quantified by a Qubit 4.0Fluorometer (Qubit Reagent: Qubit Buffer > 1:200 ratio). For library preparation, the IlluminaDNA Prep Reagent Kit and an automated liquid handler (epMotion 5075) were utilized andsequenced using the Illumina platform following the manufacturers standardprotocol. The raw reads were demultiplexed and converted into FASTQ format using Illuminasbcl2fastq software.