GSM9699872: Cont-WT-1; Mus musculus; RNA-Seq
Total RNA was extracted using MagMAX mirVana Total RNA Isolation kit. Dual-indexed mRNA libraries were prepared using the QuantSeq 3' mRNA-Seq V2 Library Prep Kit with UDI (Lexogen GmbH, Vienna, Austria), according to the user guide. According to the manufacturer's recommendations, 250 ng of starting material was used.[FM2.1] Library quality was evaluated using the QiaXcel Advanced System (Qiagen). Sequencing was performed using a NovaSeq X System (Illumina, San Diego, CA, USA, using a paired-end 2 × 150 bp configuration based on Sequencing by Synthesis (SBS).