GSM8384482: RS-Pem-POST-2-1; Homo sapiens; RNA-Seq
In brief, total RNA was extracted from patients' frozen tissues using a Qiagen RNeasy Mini Kit (Qiagen Inc.) following the manufacturer's instruction. Quantification of total RNA was performed using the Quant-iTTM RiboGreenTM RNA Assay Kit (Thermo Fisher Sci.), and quality assessment was carried out using Agilent RNA 6000 Nano Kit and the 2100 Bioanalyzer Instrument (Agilent Technologies). The cDNA was synthesized from the extracted total RNA using SureSelect cDNA Module (Agilent) and subsequently purified suing AMPure XP beads. The purified cDNA was chemically fragmented with 2X Priming Buffer (Agilent, Inc). Library preparation was carried out using the SureSelect XT Low Input Reagent Kit with indexes 1-96 (Cat: G9703A, Agilent Technologies) and automated on the Sciclone G3 NGSx Workstation (PerkinElmer, Inc.).