GSM8851841: ATAC_CD8_Day7_m38_Rep1; Mus musculus; ATAC-seq
For RNA-seq: We isolated 20,000–50,000 sorted splenic lymphocytes for RNA-sequencing. CD8+ T cells were sorted from wild-type mice at d0 (naive: TCRβ+CD8+CD62LhiCD44lo), d7, or d35 PI (effector and memory: TCRβ+CD8+CD62LloCD44hiTetramer+). For ATAC-seq, 7,000-50,000 cells were collected. Fresh cells were washed in cold PBS and lysed. Transposition occurred at 42 °C for 45 min. For RNA-seq: RNA was isolated from sorted cell populations using Trizol (Invitrogen), and total RNA was amplified using the SMART-seq V4 Ultra Low Input RNA kit (Clontech). Subsequently, 10 ng of amplified cDNA was used to prepare Illumina HiSeq libraries with the Kapa DNA library preparation chemistry (Kapa Biosystems) using eight cycles of PCR. For ATAC-seq: DNA was purified using the MinElute PCR purification kit (Qiagen) and amplified for five cycles. Additional PCR cycles were evaluated by real-time PCR. The final product was cleaned by Ampure Beads at a 1.5 × ratio.