GSM8989113: NELFC-AID-Auxin-ELOA-KO_rep1_LR; Homo sapiens; RNA-Seq
Total RNA was isolated using the RNeasy mini kit (Qiagen, #74106) as per manufacturer's instructions. Long read RNA-seq libraries were prepared using NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification Module, Iso-Seq Express oligo kit, and SMRTbell® prep kit 3.0 per manufacturer's instructions. Library quality was assessed on Agilent Femto Pulse before the libraries were loaded onto a Revio or Sequel II flow cell. Short read RNA-seq libaries were prepared using Takara Bio SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian kit (Cat.# 634485) per maufacturer's instructions. Library quality was assessed on Agilent DNA ScreenTape.