GSM8751757: LM_CnT30_1_Diff52_d32_28032022_A04_S12_R2; Homo sapiens; RNA-Seq
Total RNA was isolated using the E.Z.N.A. Total RNA Kit I (Omega Bio-Tek) 400ng of RNA per sample and the KAPA RNA HyperPrep kit with RiboErase (human/mouse/rat [HMR]) (Kapa Biosystems) were used. Fragmentation and priming was executed at 94°C for 6 min. A 1.5-μM stock (NEXTflex DNA barcodes; Bioo Scientific) was used for adapter ligation. Eight cycles of library amplification and a 0.8× bead-based cleanup. High-sensitivity DNA bioanalyzer (Agilent Technologies) was used to determine the library size, and to measure the library concentration the DeNovix double-stranded DNA (dsDNA) high-sensitivity assay was performed. Sequencing was done with the Illumina NextSeq 500 instrument and 42bp-paired end reads were generated.