GSM8709918: 35S::DXO1 (E394A+D396A)/dxo1 MS replicate 2; Arabidopsis thaliana; OTHER
Tissue of 12-day-old seedlings was snap frozen in liquid N2 and homogenized with an autoclaved and chilled mortar and pestle. Cells were lysed using Qiazol lysis reagent (Qiagen, Valencia, CA, USA) and the lysate was homogenized using Qiashredders (Qiagen, Valencia, CA, USA). Total RNA was then isolated using Qiagen miRNEasy mini columns (Qiagen, Valencia, CA, USA) as per the manufacturer's instructions. All RNA was then treated with RNase-free DNase (#79254, Qiagen, Valencia, CA, USA) at room temperature for 30 minutes followed by purification via ethanol precipitation. GMUCT libraries were constructed using an estabilished protocol (Willmann et al., 2014).