Illumina MiSeq paired end sequencing
V4 16S rRNA using primers 515F and 806RB
DNA extracts were prepared for paired-end MiSeq sequencing (Illumina, CA, USA) using a two-step PCR method to amplify the V4 hypervariable regions of bacterial and archaeal 16S rRNA genes, and to attach MiSeq adapter and barcode sequences. 