GSM9128044: SeMSNs3-GAS; Mus musculus; RNA-Seq
Extract RNA using the Boryou® Total RNA Extraction Kit. 2μg of total RNA was used to construct the sequencing libraries. The purified Total RNA was subjected to steps such as mRNA isolation, fragmentation, first-strand cDNA synthesis, second-strand cDNA synthesis, end repair, 3' end addition of A, ligation of adapters, and enrichment, to complete the construction of the mRNA sequencing library. The library concentration was detected using the Qubit 2.0 fluorescence quantitative instrument (Thermo Fisher Scientific), and the distribution of library fragments was detected using the Agilent 4200 TapeStation (Agilent Technologies).