RNA-Seq of fusarium graminearum: mycelium
Total RNA was extracted from the tissue using TRIzol Reagent according the manufacturers instructions. Then RNA quality was determined by 5300 Bioanalyser (Agilent) and quantified using the ND-2000 (NanoDrop Technologies). Only high-quality RNA sample(OD260/280=1.8~2.2, OD260/2302.0, RQN6.5, 28S:18S1.0, >1g) was used to construct sequencing library.