Metagenomic analysis of microbial communities in Sargassum-based (SBLB-INTEC)
DNA was extracted from triplicate samples of two liquid biofertilizers (SBLB-INTEC and LB-BANELINO) produced under anaerobic fermentation. Microbial biomass was collected via vacuum-assisted filtration (1.6 m and 0.2 m membranes) and preserved in TE buffer. Metagenomic DNA was extracted using the DNeasy PowerWater Kit (Qiagen). The V3-V4 region of the bacterial 16S rRNA gene was amplified using universal primers 341F (5-CCTAYGGGRBGCASCAG-3) and 806R (5-GGACTACNNNGGGGGTATCTAAT-3). Libraries were sequenced on the Illumina NovaSeq 6000 platform (250 bp paired-end reads)