GSM9196920: HEK293 , LMNA , Rep3 , ATAC; Homo sapiens; ATAC-seq
HEK293 (ATCC) were cultured in complete Dulbecco's Modified Eagle's Medium (DMEM) (Corning) supplemented with 10% fetal bovine serum ( Corning) and 1% Penicillin-Streptomycin. Cells were maintained in 10-cm dishes by seeding 1 x 10^6 cells with complete medium every 2-3 days. Cells were maintained in a humidified atmosphere at 37 C with 5% CO2. For ATAC-Seq, 100,000 cells were collected following overexpression of eGFP, LMNA, LMNB1, or LMNB2. Libraries were prepared using the ATAC-Seq Kit (Active Motif) according to the manufacturer's protocol. For small RNA-Seq, cells from the same overexpression conditions (eGFP, LMNA, LMNB1, or LMNB2) were recollected, and total RNA enriched for small RNAs was extracted using the mirVana PARIS Kit (Thermo Fisher Scientific) following the manufacturer's instructions. All sequencing was performed at the University of Illinois, Roy J. Carver Biotechnology Center.