GSM9288202: RuiS20231003MM6_mRNP_AML_MM6_END1_S4; Homo sapiens; OTHER
Genomic DNA from CRISPR-screened cell samples was extracted using the HotSHOT protocol, a rapid alkaline lysis method. The CRISPR sgRNA cassette was amplified by PCR using primers DCF01 (5′-CTTGTGGAAAGGACGAAACACCG-3′) and DCR03 (5′-CCTAGGAACAGCGGTTTAAAAAAGC-3′). The PCR product was then prepared for Illumina sequencing by adding P7 and P5 adapters and dual indexes.