GSM9635185: Thymus Macrophage, Adult C57BL/6 Mouse, 2024; Mus musculus; RNA-Seq
Thymocytes were harvested and subjected to enzymatic digestion using the Spleen Dissociation Kit (Miltenyi Biotec) and the gentleMACS™ Dissociator (Miltenyi Biotec) according to the manufacturer's instructions. The resulting cell suspensions were filtered through a 70 µm cell strainer to remove debris, resuspended in PBS, and kept on ice until further processing. Single-cell RNA-seq libraries were generated using the 10x Genomics Chromium Single Cell 3′ Gene Expression platform according to the manufacturer's protocol (10x Genomics, Pleasanton, CA). Briefly, single-cell suspensions were prepared from mouse thymus, and CD64⁺ cells were isolated by fluorescence-activated cell sorting (FACS). The sorted cells were loaded onto the Chromium Controller to achieve single-cell encapsulation in Gel Bead-in-Emulsions (GEMs). Reverse transcription, cDNA amplification, and library preparation were performed following the 10x Genomics Chromium Single Cell 3′ Reagent Kit User Guide. Libraries were sequenced on an Illumina NovaSeq 6000 system using paired-end sequencing with standard read lengths as recommended by 10x Genomics.