GSM8989059: 3T3-L1 differentiated day 7, biol rep 1, total RNA seq; Mus musculus; RNA-Seq
For collection of RNAseq samples, cells were washed with PBS followed by addition of 500uL TRIZOL (Invitrogen Cat# 15596018) to each well and RNA is extracted following manufacturer's protocol. The RNA samples were stored at -80°C until library prep and sequencing. For each 3T3-L1 cell condition,  both total RNA  and polyA enriched mRNA library construction  was processed using TruSeq Stranded Total RNA Ribo-Zero Gold and Illumina Truseq mRNA kits according to manufacturor's instructions,The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries were pooled and sequenced on NovaSeq6000, according to effective library concentration and data amount. The clustering of the index-coded samples was performed according to the manufacturer's instructions. After cluster generation, the library preparations were sequenced on a NovaSeq6000 and paired-end reads were generated.