GSM9456963: In vitro-cultured primitive macrophage progenitors (PMPs) following 12 days of doxycycline-induced NEUROG2 expression; Homo sapiens; RNA-Seq
Single nuclei were prepared using Nuclei PURE Prep kit (Sigma) following the manufacturer's protocols. Isolated nuclei suspensions were loaded onto the Chromium Controller for snRNA-seq library preparation. Libraries from 12-day samples were generated using the Chromium Next GEM Single Cell 3′ Kit v3.1, 5-day samples were processed using the Chromium GEM-X Single Cell 3′ Kit v4 in combination with the Dual Index Kit TT Set A, following the manufacturer's protocols. Library quality and fragment size distributions were assessed using an Agilent 2100 Bioanalyzer. Sequencing was performed on an Illumina NovaSeq 6000 system using S4 flow cells.