GSM9422224: CT26 cells, Oxaliplatin pool_L4; Mus musculus; OTHER
Genomic DNA was extracted from approximately 1x10^7 cells using the DNeasy Blood & Tissue Kit (Tiangen, cat no. DP304) according to the manufacturer's instructions. DNA concentration and purity were measured by NanoDrop. The sgRNA cassette was amplified from 5 µg of genomic DNA using Herculase II Fusion DNA Polymerase (Agilent) with specific primers containing Illumina adapter sequences. PCR products were purified using AMPure XP beads. The final library was quantified by Qubit and its size distribution was checked on a Bioanalyzer.