GSM8751121: Astrocyte-RiboTag pulldown replicate 1; Mus musculus; RNA-Seq
After protein enrichment using streptavidin and A/B beads, RNA was extracted from the beads and bulk/g;obal cell or bulk cortical lysates using a miRNeasy Mini Kit (Qiagen Inc., 217004) following the manufacturer's instructions. 1-3 ug of total RNA was used to generate cDNA libraries. The SMART-Seq® v4 PLUS Kit (Takara Bio) was used following the manufacturer's protocol. For small RNA-sequencing library preparation, a QIAseq miRNA NGS kit (Qiagen, 331565) was used.