GSM8566021: SMC Donor2, Quiescent, DNA; Homo sapiens; OTHER
Genomic DNA and RNA were extracted using AllPrep DNA/RNA mini kit (Qiagen). 60ug RNA and reverse-transcribed using Superscript II. The cDNA and 12 ug genomic DNA were used for PCR. PCR products were size-selected with an agarose gel, purified with HighPrep PCR reagent (MagBio Genomics, cat. no. AC60050) PCR library preparation was performed to add unique molecular identifiers (UMI) and Sample Index barcode sequences for four cycles. An additional amplification cycle was run for 30 cycles.