Chaetomium strumarium BRFC4
Genomic DNA was extracted from 7 day old actively growing cultured mycelium using the QIAGEN Genomic DNA Kit. DNA concentration was evaluated using the invitrogen Qubit 4 Fluorometer Qubit 4 (Thermo Fisher Scientific, USA), and by 0.8% agarose gel electrophoresis. After confirming DNA integrity and concentration, sequencing libraries were constructed. Library fragment sizes were assessed with an Agilent 5400 fragment analyzer system (Agilent Technologies, Santa Clara, CA, USA).