GSM8566431: IMQ treated, WT #4; Mus musculus; RNA-Seq
Cells were lysed by TRIzol reagent immediately after isolation and stored in -80  ֯C. RNA was purified by using Direct-zol RNA kits (Zymo Research) following the manufacturer's instructions and quantified by NanoDrop Microvolume Spectrophotometer (Thermo-Fisher Scientific). Illumina RNA libraries were prepared with NEBNext Ultra II RNA library preparation kit for Illumina (NEB #E7490) according to manufacturer's instructions