Whole Exome Sequencing of AML Iraqi Patients
Genomic DNA (50ng per sample) was used to construct captured libraries using the Twist Library Preparation EF Kit and Twist Human Core Exome 2.0 probes. DNA was enzymatically fragmented to ~200bp, end-repaired, A-tailed, and ligated with TruSeq-compatible dual-index adapters. Post-ligation libraries were PCR-amplified, quantified (TapeStation and PicoGreen), and pooled for hybrid capture. Target enrichment was performed via hybridization with Twist exome probes, followed by post-capture amplification. Libraries were quantified by qPCR and qualified on TapeStation. Sequencing was performed on the Illumina NovaSeq 6000 platform using paired-end 150bp reads.