Ultra-long Nanopore sequencing of Elymus ciliaris genomic DNA
High-molecular-weight DNA extracted using a modified Carlson protocol and purified with QIAGEN Genomic-tip. Library prepared using Ultra-Long DNA Sequencing Kit V14 (SQK-ULK114). Basecalling performed using Dorado v7.9.8 with High-accuracy (HAC) model.