GSM9198098: PBMC，SLE_rep3; Homo sapiens; RNA-Seq
PBMC cell suspensions were filtered using a 40 um nylon cell strainer and red blood cells were removed by 1X Red Blood Cell Lysis Solution (Thermo Fisher, Cat. no. 00-4333-57) . Dissociated cells were washed with 1x DPBS containing 2% FBS. Cells were stained with 0.4% Trypan blue (Thermo Fisher, Cat. no. 14190144) to check the viability on Countess庐 II Automated Cell Counter (Thermo Fisher). Using a Chromium Single Cell 3′ Library and Gel Bead Kit v3 (10X Genomics),cell were immediately loaded onto a Chromium Single Cell Processor(10X Genomics) for barcoding of RNA from single cell. Sequencing libraries were constructed according to the manufacturer's instructions (10× Genomics) and then sequenced in a NovaSeq 6000 sequencing system (Illumina, 20012866).