Illumina Sequencing of Eurytides calliste: adult male FC449 specimen
Libraries were generated using NEBNextDNA Library Prep Kit following manufacturers recommendations and indices were added to each sample. Genomic DNA was randomly fragmented to a size of 350 bp by shearing, then DNA fragments were end-polished, A-tailed, and ligated with the NEBNext adapter for Illumina sequencing, and further PCR enriched by P5 and indexed P7 oligos. The PCR products were purified (AMPure XP system) and the resulting libraries were analyzed for size distribution by Agilent 2100 BioAnalyzer and quantified using real-time PCR. Illumina 150 bp paired-end sequencing was run on a NovaSeq 6000 instrument to obtain about a genome depth-of-coverage of about 50x.