DNA-seq of human: Bronchoalveolar lavage fluid
Genomic DNA (1 ng) was fragmented and simultaneously ligated with sequencing adapters using Tn5 transposase (tagmentation). The tagmented products were amplified by PCR using indexed primers to introduce sample-specific barcodes and enrich the library. After PCR, libraries were purified using magnetic beads and quality-controlled by Qubit fluorometry and fragment size analysis.