16S rRNA of Cephalothrix simula metagenome sample 7
C. cf. simula samples were dissected into 20 mg cross sections. Total genomic DNA was extracted from each sample using an E.Z.N.A. Mollusc DNA Kit (Omega Bio-tek, USA). A 16S rDNA metabarcoding approach was used to investigate the biotic diversity in C. cf. simula body wall. Universal eukaryotic primers were used to target the V3-V4 variable region of the 16S rDNA. The V3-V4 16S rRNA amplicon library preparation and Illumina NovaSeq 6000 paired-end sequencing (2x250 bp)