GSM9699256: H146, Vehicle, Day9, Rep2, microC; Homo sapiens; OTHER
The Micro‑C assay was performed using the Dovetail Micro‑C Kit according to the manufacturer's instructions. Briefly, cells were crosslinked with disuccinimidyl glutarate (DSG) and formaldehyde, and the crosslinked chromatin was digested in situ with micrococcal nuclease (MNase). Following digestion, the cells were lysed and the chromatin digested fragments were bound to Chromatin Capture Beads. Later, the digested fragments were end-polished, and subjected to bridge ligation and intra-aggregate proximity ligation. Following ligation, crosslinks were reversed, proteins were degraded, and the DNA was purified. Purified DNA was used to prepare the sequencing library by end repair, adapter ligation, and DNA purification. Briefly, biotin-containing ligation products were captured using streptavidin beads, followed by index PCR amplification and size selection to generate the final sequencing-ready Micro‑C library. Libraries were sequenced on an Illumina NovaSeq 6000 platform using paired-end reads.