GSM9632579: RNA from MDA-MB-231BR cells stably expressing Control shRNA2; Homo sapiens; RNA-Seq
MDA-MB-231BR control shRNA or ACSS2 shRNA whole cell lysate was collected from the monocultures for RNA extraction. RNA was extracted using Trizol (Invitrogen) as per manufacturer's instruction from MDA-MB-231BR cells stably expressing shSCR or shACSS2 (3 biological replicates per condition). RNA-Seq and analysis was performed by BGI Tech. In this project, we sequence 6 samples used BGISEQ platform, averagely generating about 4.58G Gb bases per sample. The average mapping ratio with reference genome is 95.65%, the average mapping ratio with gene is 70.65%; 17721 genes were identified. BGI constructs RNA libraries using their proprietary DNBseq™ technology, typically involving mRNA enrichment via oligo(dT) beads, fragmentation, double-stranded cDNA synthesis, end-repair, A-tailing, and adapter ligation followed by PCR amplification. The final library is circularized for DNBSEQ platform sequencing, producing high-quality FASTQ files