TCR Amplicon of human:healthy donor PBMCs
TIRTL-seq: Healthy donor PBMCs lysed and mRNA reverse transcribed using poly-A and random hexamers. cDNA amplified with TCR V segment and C segment multiplex primers in PCR1 which was then amplified with well specific dual-index primers containing Illumina adapters in PCR2. Libraries from other experiments made using unique pairs of TCR-alpha and TCR-beta C-segment primers with inline barcodes were pooled for sequencing. This is demultiplexed data for the Expanded T cells with abTCR-oldID10 barcode.