Total RNA-sequencing of liver tumours and non-tumour liver tissue from five strains of mice and rat
Total RNA-sequencing of liver tumours and non-tumour liver tissue from five strains of mice and rat
Liver tissue and liver tumours from male mice and rats were collected and snap frozen in liquid nitrogen. Simultaneous isolation and purification of genomic DNA and total RNA from tumours, untreated normal adult liver, and DEN-exposed liver tissue was performed using the AllPrep 96 DNA/RNA Kits (Qiagen 80311), according to the manufacturerâ€™s instructions. Total RNA from P15/P56 liver tissues was extracted using QIAzol Lysis Reagent (Qiagen 79306), according to the manufacturer''s instructions.  Libraries were prepared using the TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold (Illumina).