RNA-seq of adult Ptychodera flava trunk middle pharyngeal region (genital wing amputated)
Total RNA was extracted from freshly dissected samples, homogenised in trizol, and purified using a Zymo Direct-zol RNA Miniprep kit, according to manufacturer instructions. Library preparation and sequencing  were performed by the High Throughput Sequencing Core hosted in the Biodiversity Research Center at Academia Sinica, Taipei. RNA-Seq libraries were generated from total RNA using the Illumina Stranded mRNA Prep mRNA Sample Preparation Kit with UDI indices (Illumina, USA) according to manufacturer's instructions. Surplus PCR primers were removed using AMPure XP (Beckman Coulter Life Sciences, USA). Final cDNA libraries were checked for quality and quantified using Qubit (ThermoFisher Scientific, USA) and Fragment Analyzer for size profiling (Agilent, USA), and concentration normalized using KAPA Library Quantification Kit for Illumina Platforms (Roche, USA).