RNAseq of A375: SMG1 inhibition 48h
RNA was extracted from cell lines using Nucleospin RNA/protein kit (Macherey-Nagel). RNA sequencing library preparation was done using NEBNext rRNA Depletion Kit and NEBNext Ultra II Directional RNA. Library Prep Kit. Ribosomal RNA was depleted with NEBNext rRNA Depletion Kit. Limited-cycle PCR was used for library amplification. Sequencing libraries were validated using DNA Kit on the Agilent 5600 Fragment Analyzer (Agilent Technologies).