GSM9746047: White adipocytes, WT control, isoproterenol, biological replicate 1; Mus musculus; RNA-Seq
Total RNA was extracted using Trizol reagent (Life Technologies) according to the manufacturer's protocol. RNA quality and integrity were assessed using the Bioanalyzer 2100 (Agilent Technologies). RNA-seq libraries were prepared from poly(A)-enriched mRNA using the NEBNext Ultra II RNA Library Prep Kit (New England Biolabs) following the manufacturer's protocol.