GSM9420943: FvWT,ketoconazole,rep1; Fusarium verticillioides; RNA-Seq
The mycelia were treated with or without 2 μg/mL KTC for 16 h. After harvest, the mycelia were finely grounded into powder in liquid nitrogen. RNA was extracted according to standard TRIzol method (Invitrogen, Carlsbad, CA, United States), followed by high-throughput RNA sequencing (RNA-seq) analysis of the samples that passed quality control. The library was constructed from total RNA by mRNA enrichment or rRNA depletion, followed by fragmentation, reverse transcription, and double‑stranded cDNA synthesis. After end‑repair, A‑tailing, and bubble adapter ligation, the products were PCR‑amplified and converted into single‑stranded circular DNA for sequencing.