RNA-Seg of pig: ileal chyme
0.20 g of piglet ileal chyme was weighed into a centrifuge tube. The cetyltrimethylammonium bromide (CTAB) method, referenced from Hu et al, was employed for total microbial DNA extraction. The quality of the isolated DNA were subsequently assessed. PCR amplification of the 16S rRNA gene V3-V4 region was conducted with primers 341F (5-CCTACGGGNGGCWGCAG-3) and 806R (5-GGACTACHVGGGTATCTAAT-13) under standardized amplification parameters.