L1EFH290450--BM_2.R1.raw.fastq
Total DNA was extracted from sugarcane rhizosphere soil samples using the DNeasy PowerSoil Kit (Qiagen), in accordance with the manufacturer's instructions. DNA integrity was verified via 1% agarose gel electrophoresis, and DNA concentration was determined using a Qubit 4.0 Fluorometer. Sequencing libraries were constructed using the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB): genomic DNA was first fragmented to approximately 350 bp using Covaris ultrasonication, followed sequentially by end repair, A-tailing, ligation of Illumina adapters containing unique indices, and 10 cycles of PCR amplification. Library purification was performed using AMPure XP magnetic beads. The quality and fragment size distribution of the libraries were assessed using an Agilent 2100 Bioanalyzer, and library concentrations were determined via quantitative PCR. Finally, these libraries were sequenced on an Illumina NovaSeq 6000 platform, generating 150 bp paired-end sequencing reads.