GSM9098615: Human tongue squamous cell carcinoma CAL27, siNSUN2 knock down, biol repl3; Homo sapiens; RNA-Seq
Total RNA was extracted using the TRIzol reagent (Invitrogen, CA, USA) according to the manufacturer's protocol. RNA purity and quantification were evaluated using the NanoDrop 2000 spectrophotometer (Thermo Scientific, USA). RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Then the libraries were constructed using VAHTS Universal V10 RNA-seq Library Prep Kit (Premixed Version) according to the manufacturer's instructions. The libraries were sequenced on an Illumina Novaseq 6000 platform and 150 bp paired-end reads were generated.