16s metabarcoding of Spodoptera exigual larval oral secretion
DNA extraction from OS was carried out by Microomics Systems (Barcelona, Spain) in controlled white room conditions. DNA was used for amplification of the V3V4 regions, library preparation, and Illumina MiSeq paired-end sequencing (2 x 300 bp; ~ 60,000-80,000 reads per sample). Amplification used primers SD-Bact-0341-b-S-17 (5-CCTACGGGNGGCWGCAG-3) and S-D-Bact-0785-a-A-21 (5-GACTACHVGGGTATCTAATCC-3)