GSM9666768: Standard single-cell CUT&Tag for H3K27me3 (Abcam ab6002) in peripheral blood mononuclear cells; Homo sapiens; OTHER
Cells were bound to ConA beads followed by antibody and secondary binding in bulk, split into a 96 well plate for binding barcoded pA-Tn5  and tagmentation in 300 mM Wash buffer, this was followed by split pooling on the ICELL8 and a second round of indexing during  PCR For Single cell CUT&Tag protocol see Janssens et al. 2023 Nature Protocols DOI: 10.1038/s41596-023-00905-9