GSM9668810: NuTRAP;AdQCreER, Uninjured-1; Mus musculus; RNA-Seq
Pulled down the mRNA from GFP-tagged polysomes using an anti-GFP antibody and Protein G Dynabeads. Polysomal complexes were then isolated with the Nucleospin RNA kit, which included an on-column DNase digestion step. Libraries were generated using the Takara SMART-Seq mRNA LP kit according to  the manufacturer's instructions using 20 ng RNA