16S metagenome sequencing of Phthorimaea absoluta
The entire 16S rRNA region was amplified using the 16S Barcoding Kit 1-24 (SQK-16S024, ONT). Purification to clean up all the unused barcodes and dNTPs was done using Ampure XP beads (Agencourt Bioscience) followed by pooling of all the barcoded amplicons together, loaded it in R9 flow cell (FLO-MIN106, ONT) then sequencing was done using MinION Mk1C device for 4 hours.