GSM9197632: AML-A16 patient's bone marrow cells, pre treatment; Homo sapiens; RNA-Seq
RNA was extracted using TRIzol Reagent (Invitrogen). ScRNA-seq was performed using the 10x Chromium system (10x Genomics, Pleasanton, CA). In brief, approximately 2.0 x 10^6 cells, viability >85%, were suspended in 50μl of chilled phosphate-buffered saline + 0.04% bovine serum albumin (Thermo Fisher Scientific) . Library was processed to yield scRNA-seq libraries using 10X Chromium Next Gem Single Cell 3' Reagent Kits v 3.1 (Dual Index) (10x Genomics).