RNA-Seq of Bombyx mori: Bt-treated midgut at 96 h, replicate 3
Total RNA was extracted from the midgut of Bombyx mori using Trizol reagent. mRNA was enriched using oligo(dT) beads, fragmented randomly, and reverse-transcribed into cDNA. Library construction was performed using standard protocols, followed by paired-end sequencing on the DNBSEQ-T7 platform.