Amplicon-based 16S rRNA sequencing to confirm monoculture purity
Sequencing libraries were prepared with the TruSeq DNA PCR-Free Sample Preparation Kit, and their quality was assessed using a Qubit 2.0 Fluorometer and an Agilent Bioanalyzer 2100 system. Libraries were then pooled, quantified, and sequenced on an Illumina paired-end platform, producing 250 bp raw paired-end reads at Novogene UK (Cambridge, UK).