TCR Amplicon of human: healthy donor PBMCs
Manual-TIRTL-seq: All steps done using mechanical multichannel pipettes in 96-well plate. Healthy donor PBMCs cells lysed and mRNA reverse transcribed using poly-A and random hexamers. cDNA amplified with TCR V segment and C segment multiplex primers in PCR1 which was then amplified with well specific dual-index primers containing Illumina adapters in PCR2. Libraries made with unique pairs of TCR-alpha and TCR-beta C-segment primers with inline barcodes (human abTCR-ID09). Alternating row and col well barcodes from 384-well primer plate used during PCR2.