GSM8885855: iPSC, 149br.CRX.Cherry, rep2; Homo sapiens; OTHER
On collection, organoids were washed with PBS before being cryo-embedded in OCT using liquid nitrogen cooled 2-methylbutane before storage at -80°C. Embedded organoids were sectioned to 10µm using a cryostat, onto transcriptomic slides/chips from the Visium which were then fixed for 10 minutes at -20°C using methanol. Tissue permeabilisation was optimised for 10-week-old organoids on the Visium platform at 1.5 minutes. Slides were then stained with DAPI. cDNA synthesis, amplification and library prep were all performed with the manufacturer's instructions.