MB49_3
Total RNA was extracted using the TRIzol reagent (Invitrogen, CA, USA) accordingtothemanufacturers protocol. RNA purity and quantification were evaluated using the NanoDrop2000spectrophotometer (Thermo Scientific, USA). RNA integrity was assessed usingtheAgilent2100Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Then the libraries were constructedusingVAHTS Universal V10 RNA-seq Library Prep Kit (Premixed Version) according to the manufacturersinstructions. The libraries were sequenced on an Illumina Novaseq 6000 platformand 150bppaired-endreads were generated.