GSM9745941: MUT2 a1 high; Homo sapiens; RNA-Seq
Cells were washed with 1x cold PBS followed by RNA extraction using Maxwell RSC simplyRNA Cells kit (Promega) and Maxwell RSC 48 machine. Nanodrop was used to measure RNA quantity and purity. The RNA sequencing libraries used in this study were created from polyadenylated RNA only (polyA+), sequenced at high depth (>30 Million reads). PolyA+ library generation was carried out by The Francis Crick Institute. NEBNext Ultra II Directional PolyA mRNA kit was used by the Advanced Sequencing Facility (ASF).