GSM8852989: Mammary tumor, MMTV-PyMT, rep 5; Mus musculus; RNA-Seq
RNA was prepared from ~25mg of frozen tissue using Trizol (ThermoFisher) as per manufacturer instructions. For PyMT+ cell lines, cells lines were previously generated by Gounis et al., (Gounis et al, 2024), and plated at 0.5x106 - 1x106 cells per 10cm dish, 37°C/5% CO2 for 24 hours to achieve 80% confluence the next day. Cells were washed twice in ice-cold PBS, lysed into 1mL Trizol, and RNA prepped as manufacturer instructions. Illumina TruSeq RNA Library Prep kit v2