GSM9697763: GFP+CD34+, STAT1, shJARID2,  C&R; Homo sapiens; OTHER
All cell sorting was performed using a BD Aria Ilu SORP cell sorter. For scRNA-seq, 10,000 sorted CD34⁺ cells were used to prepare gene expression (GEX) libraries according to the 10x Genomics Chromium Single Cell 3′ Reagent Kits v3 User Guide. For CITE-seq, 10,000 sorted CD34⁺ cells were processed to generate GEX libraries using the 10x Chromium Single Cell 3' Reagent Kits User Guide, v3.1 Chemistry Dual Index, with Feature Barcoding Technology for Cell Surface Protein, User Guide CG000317. For RNA sequencing, approximately 5,000 CD34⁺CD90⁺EPCR⁺CD49f⁻CD71⁻GFP⁺ cells were sorted, followed by RNA isolation using the NucleoSpin RNA XS kit (Macherey-Nagel #740902.250) and library preparation with the SMARTer Ultra Low RNA Kit (Clontech), according to the manufacturer's instructions. For CUT&Tag, 10,000–20,000 CD34⁺CD90⁺EPCR⁺CD49f⁻CD71⁻GFP⁺ cells or enriched human cord blood CD34⁺ cells were sorted and processed following the Bench-top CUT&Tag v3 protocol from Dr. Steven Henikoff's lab (DOI: dx.doi.org/10.17504/protocols.io.bcuhiwt6). For CUT&RUN, libraries were prepared using the CUTANA™ ChIC/CUT&RUN Kit (Epicypher #14-1048) and the CUTANA™ CUT&RUN Library Prep Kit (Epicypher #14-1001). scRNA-seq libraries were prepared using the 10x Genomics Chromium Single Cell 3′ GEM Library and Gel Bead Kit v3 (PN-1000075), following the manufacturer's user guide. CITE-seq libraries were constructed using the 10x Genomics Chromium Single Cell 3′ Reagent Kits v3.1 (Dual Index Chemistry) along with Feature Barcoding Technology for Cell Surface Protein, according to the Feature Barcoding User Guide (CG000317). Bulk RNA-seq libraries were generated using the SMARTer Ultra Low RNA Kit (Clontech), following the manufacturer's instructions. CUT&Tag libraries were prepared using the Bench-top CUT&Tag v3 protocol from Dr. Steven Henikoff's laboratory (DOI: 10.17504/protocols.io.bcuhiwt6). CUT&RUN libraries were prepared using the CUTANA™ ChIC/CUT&RUN Kit (Epicypher, #14-1048) and the CUTANA™ CUT&RUN Library Prep Kit (Epicypher, #14-1001), according to the manufacturer's protocols.