OpenCRISPR-1_Profiling_Replicate1_Day7
Genomic DNA was extracted from cell pellets and amplified by two-step PCR. In the first PCR, integrated target sequences including barcode regions were amplified using Q5 High-Fidelity DNA Polymerase, with > 1,000-fold library coverage. Pooled PCR products were purified and size-selected by gel extraction. In the second PCR, Illumina adaptor and index sequences were appended. Final amplicons were pooled and purified for sequencing.