TCR Amplicon of human:Convalescent SARS-CoV2 patient's CD8+ T cells
TIRTL-seq: Convalescent SARS-CoV2 patient's T cells lysed and mRNA reverse transcribed using poly-A and random hexamers. cDNA amplified with TCR V segment and C segment multiplex primers in PCR1 which was then amplified with well specific dual-index primers containing Illumina adapters in PCR2. Libraries from other experiments (baseline and acute time points) made using unique pairs of TCR-alpha and TCR-beta C-segment primers with inline barcodes were pooled for sequencing. This is demultiplexed data for the Convalescent time point with abTCR-ID04 barcode.