GSM9197470: Huh7_SC12_DMSO_3; Homo sapiens; RNA-Seq
Total RNA was isolated from cells using RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. mRNA was enriched with poly-T oligo-attached magnetic beads. RNA sequencing libraries were prepared by Novogene using Illumina-compatible protocols. mRNA was enriched with poly-T oligo-attached magnetic beads, fragmented, and converted into cDNA. The libraries were completed through end repair, A-tailing, adapter ligation, size selection, PCR amplification, and purification.