GSM9039390: tumor_NanoCa treated; Mus musculus; RNA-Seq
The samples of tumors and draining lymph nodes (TDLNs) were harvested and cut into pieces approximately 500 mm3 in the RPMI-1640 medium with 10% bovine serum, and enzymatically. The dissociated cells were subsequently passed through then filtered 40 μM Cell-Strainer (BD) and centrifuged at 600 g for 6 minutes. After removing removed, supernatant, they were suspended in 2 mL red blood ml of lysis buffer (Solarbio) on ice for 5 min to lyse minutes blood cells. After washing twice with PBS (Solarbio), the cell pellets were resuspended in sorting buffer (PBS supplemented with 1% FBS). Single-cell suspensions collected from tumor samples and draining lymph nodes were stained with antibodies against CD45 for FACS sorting, which was performed on a BD Aria III instrument. Library was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics).