DAP-Seq of Scutellaria baicalensis: SbMYB111
This DAP-seq experiment was conducted to identify the genome-wide binding sites of the transcription factor SbMYB111 inScutellaria baicalensis. Genomic DNA was isolated from 4-week-oldScutellaria baicalensisseedlings and fragmented to an average size of 100300 bp. The DNA was incubated with the SbMYB111 transcription factor, and protein-DNA complexes were enriched through affinity purification using a tag-specific antibody. The purified DNA fragments were subsequently subjected to high-throughput sequencing on the Illumina HiSeq 4000 platform. Sequencing reads were aligned to theScutellaria baicalensisreference genome (accession number: GWHAOTO00000000) using Bowtie2, and binding peaks were identified using MACS2 with a significance threshold ofq-value < 0.05. This study aims to unravel the regulatory network governed by SbMYB111 in the flavonoid secondary metabolic pathway.