HWDO_32
This study explored how multiple climate change-related environmental stressors interact with salmon gill mucosal microbiota and gill gene expression after jellyfish (A. aurita) exposure. Gill mucus samples were collected by rolling a sterile cotton swab along the right gill arch surface, and DNA was extracted. Then the v1-v3  16S rRNA gene variable regions were sequenced using the ONT MinION device and the native barcoding kit 96 (SQK-NBD112.96) version NBA_9137_v112_revH_01Dec2021, according to the manufacturer's instructions.