515F/806R region of 16S rRNA sequencing of sea water
Genomic DNA was extracted from 1,5 L of each water sample using the DNeasy PowerSoil Kit (QUIAGEN, Germany) following the manufacturers instructions. PCR reactions were carried out with Phusion High-Fidelity PCR Master Mix (ThermoFischer, Waltham, MA, USA). The library was constructed using Nextera XT Index Kit (Illumina) and size validated by running on a Bioanalyzer 2100 then sequenced using the Illumina HiSeq 2500 platform (Illumina Inc., San Diego, CA, USA) to generate paired-end (PE250) reads.