16S rRNA gene sequencing of deep sea marine sediment:T1 S5 using Illumina sequencing
DNA was extracted from sediment samples using DNeasy PowerSoil Pro DNA Extraction Kit (Qiagen) and the quality and quantity were assessed using agarose gel electrophoresis and Qubit 4.0 fluorometer. The extracted DNA was used for library preparation using QIAseq FX DNA Library Kit (Qiagen). The DNA fragments were ligated with Illumina-specific adapters followed by amplification using high-fidelity PCR master mix. The libraries were purified and quantified using Qubit dsDNA High Sensitivity assay kit. Sequencing was performed on the Illumina NovaSeq 6000 platform using a 2  150 bp paired-end sequencing approach.