Whole genome sequencing of E-TCL1 mus musculus fecal microbiome: female on chow diet pre-CLL engraftment
Total bacterial genomic DNA was purified from fecal samples using the QIAamp PowerFecal Pro DNA Kit and TissueLyser LT following manufacturer instructions. The quantity and quality of extracted DNA was assessed using the NanoDrop One Microvolume UV-Vis Spectrophotometer. Bacterial genomic DNA was stored at -20C and held for no longer than one week prior to submission for shotgun metagenomic sequencing. Sequencing libraries were prepared using the Illumina Nextera DNA Flex kit.