Effective requesting method to detect fusion transcripts in chronic myelomonocytic leukemia RNA-seq
Effective requesting method to detect fusion transcripts in chronic myelomonocytic leukemia RNA-seq
10 samples from Bone marrow or peripheral blood separated on Fycoll-Hypaque. CD14+ monocytes were then sorted from PBMCs with magnetic beads and the AutoMacs system as previously described by Merlevede et al. (2016) Total DNA was isolated using mini DNAeasy kit (Qiagen) according to the manufacturer instructions. The quantity and quality of extracted DNA was evaluated using the NanoDrop ND-1000 spectrophotometer and the Agilent 2100 BioAnalyzer. Genomic DNA (1Âµg) has been used to prepare a library for whole genome sequencing, using the Illumina TruSeq DNA PCR-Free Library Preparation Kit, according to the manufacturer's instructions.