GSM8565627: Untrained1, D.Hipp, Spatial; Mus musculus; OTHER
mRNA was extracted and libraries were prepared following the Visium Spatial Gene Expression User Guide (Genomics, 2023). Libraries were indexed using the Dual Index Kit TT Set A (PN-1000215). Indexed libraries were loaded at 300 pM and sequenced on a NovaSeq 6000 System (Illumina) using a NovaSeq S4 Reagent Kit (200 cycles, catalog no. 20027466, Illumina), targeting a sequencing depth of approximately 2.0 × 108 read-pairs per sample.