J19 (CRX2017271; CRA030332)
Total soil DNA was extracted using the PowerSoil DNA Isolation Kit. The extracted DNA was quality-checked using Qubit and Nanodrop, fragmented to approximately 400 bp via ultrasonication, and used to construct a sequencing library with the NEBNext Ultra Library Prep Kit. The library was ultimately sequenced on the Illumina HiSeq 2500 platform with a PE150 strategy.