RNA-seq of B16 melanoma cell line, CAP replicate 1
mRNA was enriched using oligo(dT) beads, fragmented, and reverse-transcribed into cDNA. Libraries were end-repaired, adaptor-ligated, PCR-amplified, circularized, and sequenced via cPAS on a DNB-based platform. Reads were filtered (SOAPnuke), aligned (HISAT2/Bowtie2), quantified (RSEM), and analyzed for differential expression (DESeq2).