GSM9744464: HCC1937_3; Homo sapiens; RNA-Seq
Total RNA was extracted using RNeasy Mini Kit (74104, Qiagen) according to the manufacturer's instructions. ScriptSeq Complete Gold Kit (Human / Mouse / Rat) (BG1224, Epicentre) was used for RNA-sequencing library preparation from 1000ng total RNA. Ribosomal RNA was first removed from the total RNA using Ribo-Zero Gold rRNA Removal reagents (RZG1224/MRZ11124C, Epicentre), after which the ScriptSeq v2 RNA-Seq Library Preparation Kit (SSV21124, Epicentre) was used to make the Rna-seq library from the Ribo-Zero treated RNA. The rRNA-depleted RNA was fragmented chemically, and the libraries were prepared according to the manufacturer's instructions. Finally, the libraries were assessed using Agilent High Sensitivity D1000 ScreenTape Assay with 4200 TapeStation System.