GSM9421598: cKO-6w; Mus musculus; RNA-Seq
6 weeks after  adult Rack1(F/F) and Rack1(F/F;Mx1-Cre) mice injected with poly(I:C), BM cells were flushed out of the BM cavity of both femurs using a 5 mL syringe with ice-cold PBS, followed by filtration through a 70-µm strainer. BM cells were stained with PE-conjugated antibody against c-Kit and FITC-conjugated antibodies against CD3, B220, CD11b, Gr-1, and Ter-119 in PBS containing 2% FBS for 30 min on ice in the dark. Samples were washed once in staining buffer. Then, the cells were incubated with anti-PE microbeads and all c-Kit+ cells were enriched using an MS column. After that, Lin−/lowcKit+ DAPI- BM singlets were sorted by FACS and subjected to scRNA-seq. Libraries were produced with a Chromium system (10×Genomics, PN120263) following the manufacture's instruction (https://support.10xgenomics.com/single-cell-gene-expression/library-prep/doc/technical-note-assay-scheme-and-configuration-of-chromium-single-cell-3-v2-libraries)