RNA-seq comparison of Pax6.1(5a) Isoform knockout and Oryzias latipes wild type
RNA-seq comparison of Pax6.1(5a) Isoform knockout and Oryzias latipes wild type
WT and Pax6.1-5a-/- knockout embryos collected, incubated to develop into stage 24 and dechorionated. Total RNA was isolated using TRIzolTM Reagent according to TRIzolTM Reagent User Guide. Quality and purity of isolated RNA were determined by 1% of agarose gels. RNA integrity was evaluated via Agilent Bioanalyzer 2100 system using Eukaryote Total RNA Nano and Eukaryote Total RNA Pico assays. The libraries were prepared using the Illumina TruSeq Stranded Total RNA kit in the automation system Biomek FxP.