Bulk RNA-sequencing of mouse back epidermis: p120KO replicate 3
Total RNA was isolated from mechanically separated postnatal day 3 mouse epidermis using Qiagen RNeasy kits, including proteinase K digestion and on-column DNase treatment. RNA quality was assessed using an Agilent TapeStation system. Stranded mRNA-seq libraries were prepared using the Illumina Stranded mRNA Library Prep Kit and sequenced on an Illumina NextSeq 2000 to generate 100 bp single-end reads, with approximately 2030 million reads per sample.