Winter_S2
Library preparation was performed using the Ion 16S Metagenomics Kit (Thermo Fisher Scientific, USA), which employs two primer pools to amplify seven hypervariable regions (V2, V3, V4, V6V9) of the bacterial 16S rRNA gene. Following amplification, libraries were processed using the Ion Plus Fragment Library Kit and the Ion Xpress Barcode Adapter Kit for end repair and barcode adapter ligation. Individual libraries were normalized to approximately 10 pM prior to pooling. The pooled libraries were subjected to emulsion PCRbased clonal amplification and enrichment using the Ion 520 & Ion 530 Kit in combination with the Ion OneTouch 2 System. Enriched template-positive particles were then loaded onto an Ion 530 Chip and sequenced on the Ion GeneStudio S5 System (Thermo Fisher Scientific, USA)