GSM9155554: PBMCs, donor1, 6 month cryopreservation; Homo sapiens; RNA-Seq
Briefly, 30 mL of leucocytes suspension was laid on to 10 mL of Lymphocyte Separation Medium (MP Biomedicals, Fountain Pkwy, Solon, OH, USA) and span at 700 xg for 30 min at room temperature with the brake off. The lymphocyte layers were corrected and then washed with PBS (Quality Biological Inc, Gaithersburg, MD, USA) three times at 500 xg for 5 min at room temperature. The scRNA-Seq libraries were constructed using the Chromium Next GEM Single Cell 5′ Reagent Kit V2 (Dual Index) (10x Genomics, Pleasanton, CA, USA) following the manufacturer's instructions.