GSM9127585: Patient 2, bone marrow-derived mesenchymal stem cells, Control, H3K4me3 (Input); Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 15 minutes. After quenching and cell lysis, chromatin was sonicated using Bioruptor Pico sonication device (Diagenode) Libraries were prepared using IP-Star® Compact Automated System (Diagenode) from input and ChIP'd DNA using MicroPlex Library Preparation Kit v2 (12 indices) (Diagenode). Optimal library amplification was assessed by qPCR using KAPA SYBR® FAST (Sigma-Aldrich) on LightCycler® 96 System (Roche) and by using High Sensitivity NGS Fragment Analysis Kit (DNF-474) on a Fragment Analyzer™ (Agilent). Libraries were then purified using Agencourt® AMPure® XP (Beckman Coulter) and quantified using Qubit™ dsDNA HS Assay Kit (Thermo Fisher Scientific, Q32854). Finally their fragment size was analised by High Sensitivity NGS Fragment Analysis Kit (DNF-474) on a Fragment Analyzer™ (Agilent).