High throughput amplicon sequencing of of protist diversity, in various environments potentially hosting Ca. Babelota
Nucleic acids were subjected to a PCR with a high fidelity Q5 polymerase, with 30 cycles, in large volume (50 L) duplicates. Pools were checked and quantified using QuBit. Libraries were prepared using native barcoding kit V14, and loaded on an R10.4 flowcell. Sequencing was perfomed on an Mk1C using the high accuracy mode.