GSM9197745: H9 TRE3G 2xFLAG-GSX2 WT 12hr -Dox Replicate 1; Homo sapiens; ATAC-seq
Monolayers were dissociated for 30 minutes with Accutase and then lysed for 3 minutes on ice. Nuclei were tagmented for 30 minutes at 37C in a 800rpm thermomixer. Libraries were prepared using NEBNext Ultra II Q5 PCR Mastermix (NEB) for 6-11 total PCR cycles. Optimal number of cycles was determined by qPCR. Libraries were size-selected for 150-800 bp and then Agilent high-sensitivity D1000 screentape was used for QC.