GSM9666159: Multiome E8.5 embryonic and extraembryonic cells, ATAC, Biol high fat rep 1; Mus musculus; ATAC-seq
Single nuceli were extracted according to Chromium Nuclei Isolation Kit. Single nuclei were encapsulated in Gel Beads-in-emulsion (GEMs) using the Single Nuclei Multiome ATAC + Gene Expression v1 kit (The Princess Margaret Genomics Centre (PMGC) Chromium 10x Genomics Next GEM Single Cell Multiome prep v1.0 kit was used to generate libraries following manufactor's instructions. Sequencing using 100 cycle v1.5 kit 10x custom sequencing configuration