16SrRNA_V4_nasopharyngeal_infants_SouthAfrica
Nucleic acid extraction using DSP Virus/Pathogen Mini Kit (catalogue no. 937036, Qiagen GmbH, Hilden, Germany). Two-step amplification of V4 region of 16S rRNA gene. Sequencing using MiSeq Reagent Kit v3 (600-cycle) Reagent Cartridge (Illumina, San Diego, CA, USA) on Illumina MiSeqTM platform.