Transcriptome and epigenome dynamics of cold stress priming in Arabidopsis thaliana.
Transcriptome and epigenome dynamics of cold stress priming in Arabidopsis thaliana.
RNA-seq was performed for 25-days-old Arabidopsis thaliana plants grown in control conditions (22°C) (non-primed, C), plants primed with cold stress at 4°C for 3 days (Priming only, P), plants triggered with cold stress at 4°C for 3 days (Triggering only, T), and the primed and triggered plants (Priming and Triggering, PT). Leaf samples of the plants were collected. Plants were grown in 0.5x Murashige and Skoog (MS) media with 0.8% agar with 0.1% sucrose content at 22°C with a long day (16 h light / 8 h dark) for 9 days, then transferred to soil for stress treatment and until the sample collection. Total RNA was extracted from 100 mg of frozen samples with the Maxwell® 16 LEV Plant RNA Kit (Promega Corporation, USA) following instructions from the manufacturer.  RNA libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, USA). 