GSM8818373: JM275B,tumor; Homo sapiens; RNA-Seq
RNA was extracted from FFPE patient samples using Qiagen AllPrep DNA/RNA FFPE Kit (80234). After RiboGreen quantificaiton and quality control by Agilent BioAnalyzer 0.1-1 µg of total RNA was used for construction of sequencing libraries. Library prepration was done using TruSeq Stranded Total RNA Kit (Illumina catalog # RS-122-1202) according to instructions provided by the manufacturer with 8 cycles of PCR.  Samples were barcoded and run on a NovaSeq 6000 in a PE100 run, using the NovaSeq 6000 S4 Reagent Kit (200 Cycles) (Illumina).  On average, 96 million paired reads were generated per sample and 40% of the data mapped to the transcriptome