Role of strigolactones in response to wounding in Arabidopsis
Role of strigolactones in response to wounding in Arabidopsis
For RNA-isolation analyses, plant tissue (shoot or root) was collected in four biological replicates, each containing tissue from 4 seedlings.  For the RNAseq experiment, plants were grown on Perti dish with 1/2 MS up to 28 days. 12 plants were placed on the single Petri dish. Plants were placed in the greenhouse under a 20/18°C day/night, 16/8 photoperiod and 420 μE m−2 s−1 light intensity. Samples were frozen immediately in liquid nitrogen; RNA was isolated using the mirVana miRNA Isolation Kit (Sigma, catalogue number: AM1560).  Preparation of RNA library was conducted by Novogene Co., LTD (Beijing, China). 