16S rRNA sequencing of human gut microbiome: Bu-H-L-pH6.5 1 d4.7 Pacbio
Genomic DNA was extracted from human fecal-derived bioreactor samples and normalized to 0.5 ng/L. Full-length 16S rRNA genes were amplified using PacBio 16S Kinnex barcoded primers according to PacBio's Amplification of bacterial full-length 16S gene protocol. Amplicon libraries were constructed using the SMRTbell Prep Kit 3.0 and purified with SMRTbell cleanup beads. Equimolar pooled libraries were sequenced on a PacBio Revio system. High-fidelity (HiFi) reads were generated and demultiplexed with lima, followed by quality control with FastQC and summary reporting using MultiQC.