Small RNA-seq and RNA-seq of canine pulmonary adenocarcinoma and healthy lung tissues
Prepared by Azenta Life Sciences. RNA was fragmented by thermal incubation at 94C for 15 minutes. Libraries were constructed with VAHTS mRNA Capture Beads and the VAHTS Universal V8 RNA-seq Library Prep Kit for Illumina (Vazyme Biotech Co., Ltd., Nanjing, China) in accordance with the manufacturers instructions, and sequencing was performed on an Illumina NovaSeq 6000 (Illumina Inc.) in paired-end 150-bp mode.