ONT ultra-long sequencing of Cephalopholis sonnerati muscle
The long-fragment genomic DNA from muscle tissue was generated using a sodium dodecyl sulfate extraction method. The library was constructed using the Ligation Sequencing Kit (SQK-LSK110), and the purified library was loaded onto primed R9.4 Spot-On Flow Cells and sequenced using the PromethION sequencer (Oxford Nanopore Technologies, UK).