GSM9128354: 2-mono-il10; Homo sapiens; RNA-Seq
Qiagen RNeasy Mini Kit was used to perform RNA isolation (RNA concentration > 10 ng/µL and RIN>5 Libraries are prepared with Watchmaker mRNA Library Prep Kit for Illumina  protocol according supplier recommendations. Briefly the key stages of this protocol are successively, the selection of PolyA containing mRNA molecules using poly-T oligo attached magnetic beads from 100ng total RNA, a fragmentation to obtain approximately 300bp pieces, double strand cDNA synthesis and finally Illumina adapters ligation and cDNA library amplification by PCR for sequencing. The sequencing was performed on an Illumina NovaSeq X Plus sequencer as paired end 2x100 bp reads.