GSM9697928: Cd4-Cre Rpa1fl/fl CD8+ T cells, GE; Mus musculus; RNA-Seq
Nuclei were isolated using a lysis buffer according to the manufacturer's instructions. Nuclei integrity and concentration were assessed using AO/PI staining and microscopy, and samples with intact nuclear morphology and minimal debris were used for downstream applications. Single-cell ATAC-seq libraries were generated using the SeekOne DD Single Cell Genome Multi-omics (ATAC + RNA) Kit according to the manufacturer's instructions.