GSM8751462: eAML4.1 GEX; Homo sapiens; RNA-Seq
Digestion of tissue and cryopreservation in FCS with 10% DMSO until analysis. Thawing in PBS supplemented with 10% FCS and 10% bovine DNase I, grade II. Resuspension in PBS with 0.04% ultra-pure BSA. Standard 5' 10x Genomics single cell RNA sequencing protocol according to manufacturer's instructions. TCR-seq using V(D)J amplification kit.