GSM9635581: saline_input_rep2; Mus musculus; RIP-Seq
RNAs were extracted by douncing the frozen brain tissue in ice-cold RIPA buffer (Thermo, 89901). Both input and immunoprecipidated RNAs were end-repaired with T4 PNK  (Thermo, EK0031). Libraries were constructed with NEBNext® Small RNA Library Prep Set for Illumina®  (NEB, E7330S) according to manufacturer's instructions.