mus musculus: 4-5 weeks male brain
Total RNA was extracted using TRIzol reagent. mRNA was enriched using oligo(dT) magnetic beads. The enriched mRNA was fragmented, then reverse transcribed into double-stranded cDNA. End repair, A-tailing, adapter ligation, and PCR amplification were performed using the Illumina TruSeq Stranded mRNA Library Prep Kit. Library quality was assessed on an Agilent 2100 Bioanalyzer, and sequencing was performed on an Illumina NovaSeq 6000 platform.