GSM8413561: Dp16; Mus musculus; OTHER
Mouse OCT-embedded hemispheres (anteroposterior from bregma: +1 to -2 mm) from each experimental group at 9-months of age, were snap-frozen in isopentane pre-cooled with dry ice and liquid nitrogen. Brains were sectioned on the cryostat at 10 um thickness using a CryoStar NX70 cryostat (ThermoFisher). Sections were prepared according to the Visium Spatial Gene Expression Reagent Kit (10X Genomics, PN-1000187). Sections were fixed in methanol (Sigma) for 30 min at -20 C, then treated for 1 min with isopropanol and air-dried. To stain the tissue, sections were incubated in Mayer s Hematoxylin (Dako) for 7 min at room temperature (RT), then Bluing buffer for 2 min and Eosin (Sigma) for 1 min at RT. After drying, H&E images of sections were taken using an EVOS M7000 Invitrogen microscope (ThermoFisher Scientific).