GSM9421136: KYSE150-normoxia-HIF1α; Homo sapiens; OTHER
For CUT&Tag experiment, 100,000 cells were collected for CUT&Tag followed manuals (cat. #N259-YH01, Novoprotein Inc.) to get HIF1α-enriched DNA. DNA libraries were prepared through PCR using a commercial  CUT&Tag kit (cat. #N259-YH01, Novoprotein Inc.) according to the manufacture's protocol. The sequencing libraries were qualified with Qubit 2.0 and Agilent 2100, and sequenced on an Illumina NovaSeq 6000 platform for each library.