GSM9420248: Grade 0R ACR Rep7; Homo sapiens; ncRNA-Seq
RNA extraction was carried out using NucleoSpin® miRNA Plasma of Macherey Nagel, following the protocol and instructions provided by the manufacturer. RNA quantification was performed using a NanoDrop 1000 spectrophotometer and the Qubit 3.0 fluorometer (Thermo Fisher Scientific). The purity and integrity of RNA samples were determined using the RNA 6000 Nano Kit and Small RNA Kit with 0.8% agarose gel and the Agilent 2100 Bioanalyzer (Agilent Technologies). cDNA libraries were obtained following Illumina's recommendations. Briefly, 3′ and 5′ adapters were sequentially ligated to RNA prior to reverse transcription and cDNA synthesis. Size selection was performed using a 6% polyacrylamide gel. The quality and quantity of cDNA libraries were analyzed using the High-Sensitivity D1000 ScreenTape Assay and the 4200 TapeStation System (Agilent Technologies). cDNA libraries were then pooled and sequenced by two lanes of 100 bp paired-end sequencing using an Illumina HiSeq 2500 sequencer.