RNASeq of Homo sapiens sample Ishikawa AKT 3
RNAs were ribodepleted using RiboGone Mammalian (Takara Bio). cDNA libraries were constructed with NEBNext Ultra II Direction RNA Library Prep Kit for Illumina (New England Biolabs). During adaptor ligation, NEBNext Adaptors were replaced with Singular Genomics Universal Library Prep Adapter. During index PCR, NEBNext oligos were substituted with Singular Genomics Dual Index PCR Primers. Libraries were sequenced on a Singular Genomics G4 instrument by the Analytical and Translational Genomics Shared Resources at UNMCCC