GSM9369721: RUO-OSC-V lot 57; Homo sapiens; RNA-Seq
scRNA-seq was performed to establish comparability between resultant OSCs that were generated under various differentiation conditions, as well as evaluate the consistency of different batches generated using the same condition. OSCs were cryopreserved in CryoStor CS10 (StemCell Technologies) prior to being processed for single-cell RNA sequencing by Genewiz (Azenta). Cells were loaded onto a Chromium Single Cell Chip (10x Genomics) and processed through the Chromium Controller to generate single-cell gel beads in emulsion. scRNA-seq libraries were generated using the Chromium Single Cell 3′ Library & Gel Bead Kit (10x Genomics). Target cell recovery was estimated to be 3,000 cells per sample. Files were generated following a split-set analysis workflow.