RNA-seq of BxPC-3 sgNC-Poly
For RNA-seq, mRNA was fragmented by heating at 94C for 25 min in 1 PNK (T4 Polynucleotide Kinase) buffer (NEB). RNA fragments were end-repaired using T4 PNK, purified using the RNA Clean and Concentrator kit (Zymo Research), and libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB). Libraries were sequenced on an Illumina NovaSeq X platform.