GSM9197849: Cotesia vestalis ovary, day 2, replicate2; Cotesia vestalis; RNA-Seq
Each of the 15 ovary samples were ground in RNA-easy Isolation reagent (Vazyme Biotech Co., Ltd, Nanjing, China) on dry ice, and total RNA was subsequently extracted using an RNA easy Isolation reagent kit according to the manufacturer's protocol. The RNA integrity value (RIN) was accessed using a Bioanalyzer 2100 (Agilent Technologies Co., Ltd, Palo Alto, CA, USA) followed by 1.1% agarose gel electrophoresis to evaluate the total RNA quality. RNA samples with RIN > 8 were retained for subsequent analyses. RNA-seq libraries were prepared from 1 µg of total RNA per sample, following the manufacturer's protocol (Illumina Inc., San Diego, CA, USA).