Whole genome sequencing of Curculio nanulus: larva whole body
High molecular weight genomic DNA was extracted from C. nanulus using the Qiagen genomic tip DNA extraction kit. High molecular weight DNA was sheared to 18kbp with a Diagenode Megaruptor, and the BluePippin system (Sage Science, Beverly, MA, USA) was used to collect fractions containing >15-Kbp fragments for library preparation. Genomic libraries were prepared following the SMRTbell Express Template Prep. Kit 2.0 protocol (PacBio, Menlo Park, CA, USA).