GSM8853039: MEF n6 control  rep 6; Mus musculus; RNA-Seq
MEFs were plated at 1x106 cells per 10cm2 dish in full growth media (DMEM (ThermoFisher) supplemented with 10% FBS, 2mM L-glutamine (ThermoFisher) and 100U/mL penicillin/streptomycin (ThermoFisher)) supplemented with 10 or 30µM uracil (Sigma) and incubated at 37°C/5% CO2 for 24 hours. Cells were then washed with ice cold PBS, scraped into Trizol, and RNA prepped as per manufacturers instructions. Illumina TruSeq Stranded Total RNA Lib Prep, Gold kit. Quality and quantity of the DNA libraries was assessed on an Agilent 2200 Tapestation.