PAR-CLIP analysis of EBV positive LCLs: LCLWT, replicate 1
Established LCLs (LCLD2 and LCLWT) were expanded in log phase and cultured in 100 uM 4-SU (4-thiouridine) for 24 hr prior to cross-linking at UV 365 nm. Ago-bound RNAs were immunopurified using antibodies to Ago2 (Abcam). cDNA libraries were sequenced on the Illumina platform.