GSM9126365: S462 cell line, control, replicate 1; Homo sapiens; RNA-Seq
RNA was isolated using RNeasy Mini Kit (QIAGEN). The quality of Total RNA was checked by Agilent Fragment Analyzer (Agilent Technologies, Santa Clara, CA), and only high-quality RNA samples (RQN >7) were used for mRNA-seq library preparation and sequencing. Following the Illumina TruSeq stranded mRNA sample preparation guide, we used approximately 500ng Total RNA for RNA-seq library preparation. fter RNA-seq libraries were quantified, they were pooled and subsequently loaded for 101bp paired read sequencing run on the Illumina NovaSeq 6000 platform.