GSM9842810: c57-D10-lung-M2-R1; Mus musculus; RNA-Seq
Mouse tissue samples were collected, homogenize, and preserved in TRIzol reagent (Invitrogen/Thermo Fisher) by the submitter. Preserved samples were shipped to Azenta Life Sciences (GENEWIZ) on dry ice. Total RNA was then extracted by Azenta using a column-based purification method (Qiagen RNeasy or equivalent best-in-class silica-membrane kit with on-column DNase I treatment) according to the manufacturer's instructions and Azenta's optimized protocols for TRIzol-preserved samples. Library preparation was performed by Azenta Life Sciences using a stranded polyA-enriched bulk RNA-seq protocol, based on Illumina or 10x Genomics-compatible reagents (confirm with Azenta report).