GSM9421141: KYSE150-hypoxia; Homo sapiens; ATAC-seq
For ATAC-seq experiment, 50,000 KYSE30 cells were collected for ATAC-seq followed commercial manual ATAC kit (cat. #N248, Novoprotein Inc.). DNA libraries were prepared through PCR using a commercial ATAC kit (cat. #N248, Novoprotein Inc.) according to the manufacture's protocol. The sequencing libraries were qualified with Qubit 2.0 and Agilent 2100, and sequenced on an Illumina NovaSeq 6000 platform for each library.