16S rRNA gene sequencing of Mansonia mosquitoes: pooled adult females from coconut plantations in central Thailand
Genomic DNA was extracted from pooled abdomens of three Mansonia species using the FavorPrep Tissue Genomic DNA Extraction Kit. Amplicon libraries of the bacterial 16S rRNA V3V4 regions were generated with the MetaVX Library Preparation Kit using primers 341F/806R, purified, and pooled in equimolar ratios. Sequencing was performed on an Illumina MiSeq platform (2  300 bp, paired-end) with dual indexing, and raw reads were processed to remove adapters, low-quality sequences, and chimeras before downstream microbiome analysis.