Gene expression dynamics in Beijerinckiaceae bacterium RH AL1 in response to different lanthanum compounds and different lanthanide-containing minerals
Gene expression dynamics in Beijerinckiaceae bacterium RH AL1 in response to different lanthanum compounds and different lanthanide-containing minerals
Biomass was harvested from mid-exponential cultures by means of centrifugation. Beijerinckiaceae bacterium RH AL1 was grown using MM2 medium to mid-exponential phase, using methanol (0.5%, v/v; 123 mM) as carbon and energy source. Cultures were supplemented with either different lanthanum compounds (LaCl3, La2O3, LaPO4; 1 ÂµM total lanthanum) or lanthanide-containing minerals/alloys as lanthanide source. The following minerals/alloys have been used: bastnaesite, gadolinite, loparite, monazite, xenotime, ferrocerium. For incubations with ferrocerium one piece of ferrocerium (~ 145 mg) was added. For incubations with a mineral lanthanide source, we used 5 mg of the respective mineral.  Total RNA was extracted with TRI reagent (Applied Biosystems), mRNA was enriched using the RiboMinus bacteria 2.0 transcriptome isolation kit (Invitrogen). Sequencing libraries were prepared with the NEBNext Ultra II Directional RNA library prep kit for Illumina (New England Biolabs).