GSM9746069: Day2 limb mesenchymal organoids, NC/FW Multiplex pool; Mus musculus; RNA-Seq
Single-cell suspensions were prepared from embryonic mouse limb mesenchyme or cultured limb-mesenchymal organoids/micromass samples. Cells were processed using the 10x Genomics Chromium Next GEM Single Cell 3′ v3.1 workflow with Feature Barcode/CellPlex multiplexing according to the manufacturer's instructions. Gene-expression and feature-barcode libraries were generated from the same pooled single-cell suspensions. Single-cell gene-expression libraries were prepared using the 10x Genomics Chromium Next GEM Single Cell 3′ v3.1 platform. Feature Barcode/CellPlex libraries were prepared using the Chromium Single Cell 3′ Feature Barcode Library Kit. Gene-expression libraries were indexed with Dual Index Kit TT Set A, and Feature Barcode libraries were indexed with Dual Index Kit NT Set A. Libraries were sequenced on an Illumina NovaSeq 6000 using an SP 100-cycle kit with Read 1 = 28 bp, Read 2 = 90 bp, Index 1 = 10 bp, and Index 2 = 10 bp.