HiFi sequencing data of Impatiens mengtszeana
The purified DNA samples were fragmented using g-TUBE. Following fragmentation, the DNA underwent damage repair and end repair using the SMRTbell Prep Kit 3.0. Hairpin adaptors were then ligated to both ends of the DNA fragments, generating single-stranded circular structures known as SMRTbell templates. The BluePippin fully automated nucleic acid electrophoresis system was used for fragment size selection to prepare the final SMRTbell library. Circular consensus sequencing (CCS) was performed on the PacBio Sequel II platform (Pacific Biosciences, Menlo Park, CA, USA) to produce raw reads.