Illumina sequencing of Wolbachia endosymbiont of Rhagoletis indifferens wCin2-PNW1: Wolbachia reads
An Illumina library was prepared from 200 ng of HMW DNA from a single Rhagoletis fly, which the remaining HMW DNA was used for Nanopore sequencing. The library was prepared using the nebnext ultra II kits by the Notre Dame genomics core and sequenced at BGI on a HiSeq X Ten. Reads were quality filtered to q20 and length 80 bp and mapped to the assembled Wolbachia genome for Pilon polishing.