WGBS of Poecilia reticulata: whole brain tissue
We extracted DNA from whole brains using AllPrep DNA/RNA Kits (Qiagen, Hilden, Germany) following the manufacturers protocol. Whole genome bisuflite (WGBS) library preparation and sequencing was performed at the McGill Genome Center (Montral, Canada). Paired end libraries of 150 bp long reads were prepared for each fish and sequenced on two lanes of the Illumina NovaSeq6000 S4 (Illumina, San Diego, United States) with guppy samples from a different project, with 69 individuals pooled per lane.