GSM8815472: zygotes_h3k27me3_bulk_CUT&RUN_r2; Mus musculus; OTHER
Zygotes were collected at 23 post-hCG treatment in M2 medium. Cumulus cells were removed using M2 media containing hyaluronidase (Merck-Millipore MR-051-F). The zona pellucida was removed with Pronase (SIGMA P8811), followed by two washes in M2 medium. Bulk CUT&RUN as described in (Moutaux& Schwager et al., 2025)