RNA-seq of Ixodes scapularis larvae during Borrelia burgdorferi acquisition
Larvae were fed on non-infected mice. After detachment from the mice, larvae were collected and the total RNA extraction was performed with the AllPrep DNA/RNA/Protein Mini Kit (QIAGEN) according to manufacturer`s specifications.The library was constructed using the NEBNextUltraTM II (Directional) RNA with polyA selection library prep kit and sequencing was performed in an Illumina Novaseq 6000 DNA sequencer.