GSM8436445: RS25_891_Distal Colon; Mus musculus; RNA-Seq
RNA was extracted from tissues using Zymo Tri-Reagent and Invitrogen PureLink RNA isolation kits followed by further purification using Zymo RNA Clean and Concentrate kits. Libraries were prepared using Illumina Stranded mRNA Prep, Ligation kit with IDT for Illumina Unique Dual Indexes following manufacturer's recommendations, except that half reaction volumes were used. Completed libraries were QC'd and quantified using a combination of Qubit dsDNA HS and Agilent 4200 TapeStation HS DNA1000 assays. The libraries were pooled in equimolar amounts for multiplexed sequencing, and the pool quantified using the Invitrogen Collibri Quantification qPCR kit.