GSM9725436: TMD8 Cells,SF3B1 A428D Clone 6,Pomalidomide-Venetoclax Treatment; Homo sapiens; RNA-Seq
After 24h of drug treatment the cells were pelleted, lysed using Zymo DNA/RNA shield, and submitted to Plasmidsaurus for Illumina-based 3' bulk RNA sequencing. Library construction and Illumina sequencing were performed by Plasmidsaurus. According to their website: "We extract total RNA using a bead-based extraction approach from cells preserved in Zymo DNA/RNA Shield. We assess post-extraction concentration using a fluorescence based plate reader assay and normalize sample concentrations before proceeding. For sequencing library preparation, we convert mRNA into complementary DNA (cDNA) via reverse transcription and second-strand synthesis, followed by tagmentation, library indexing, and amplification."