RNAseq of primary fibroblast-induced neurons from people living with and without HIV
At day 21 of differentiation, PSA-NCAM iNs were purified by fluorescence-activated cell sorting (FACS) using a BD FACSymphony S6. Sorted live (DAPI) cells were pelleted and lysed for RNA extraction using column-based purification (Qiagen RNeasy) following manufacturer instructions. RNA quality and quantity were verified by Bioanalyzer prior to library preparation. Matching UNA-fibroblast RNA was collected in parallel from the same passage cultures to control for passage effects.