RNA-Seq of HT29: CBLL1 Knockdown, monolayer
RT: SuperScript VILO cDNA Synthesis Kit. Pre-loaded IonCode 96-well PCR plate. cDNA libraries: Ion AmpliSeq Kit for Chef DL8 (Thermo Fisher Scientific) - Ion Chef system, Ion AmpliSeq Transcriptome Human Gene Expression Panel (A31446). Ion 540 Chip (Thermo Fisher Scientific). Reference: human genome version 19 (GRCh37).