WGS of mouse heart replicate 2
For whole genome sequencing of mouse tissues, we performed dissections of heart and cerebellum from two adult (8 weeks old) C57/Bl6 mice. The same region of tissue was quickly dissected from euthanized mice and cut into small chunks of approximately 50mg in weight. From cerebellum, the brain was first removed and then the cerebellum was micro-dissected on ice to keep the tissue cold until ready for digestion. Once the tissue was isolated it was immediately moved to digestion using DNeasy Blood and Tissue Kits (Qiagen) following manufacturer protocols for tissue digestion. After tissue digestion DNA was isolated following manufacturer protocols and final DNA isolation was resuspended in water. DNA was quality controlled by quantifying concentration on a nanodrop and then run an Agilent bioanalyzer. Following QC whole genome libraries were prepared as described below.