Isolation and characterization of Pseudomonas aeruginosa from jute, kenaf, and mesta plant tissues
Genomic DNA was extracted from bacterial isolates using a standard bacterial DNA extraction protocol. Sequencing libraries were prepared using a paired-end library preparation kit following the manufacturers instructions. DNA quality and quantity were assessed prior to library construction. Fragmentation was performed enzymatically, followed by end repair, A-tailing, and adapter ligation. Ligated products were purified and enriched by PCR amplification. Final libraries were quantified and quality-checked before sequencing.