Single cell RNAseq of wild-type and Iqgap2-/- (KO) mouse brain endothelial cells
Single cell RNAseq of wild-type and Iqgap2-/- (KO) mouse brain endothelial cells
Mouse brain cortices were dissociated to single cell suspension and labelled with anti-CD31 antibody. Single cell suspension of CD31+ cells was collected as sample and submitted for sequencing. Nucleic acid was barcoded and extracted from cells using standard protocols for Chromium Next GEM Single cell 5' kit. Standard Chromium Single cell 5' Gene Expression Dual Index libraries were used.