GSM9198805: Ketogenic Diet,Resection,KETO15; Homo sapiens; RNA-Seq
RNA was extracted from the biopsies and tumor resections using the Maxwell RSC simplyRNA Tissue Kit according to the manufacturer's protocol. The quality and quantity of extracted RNA were assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Tumor RNA samples were selected for library preparation based on the RNA Integrity Number (RIN). Libraries were prepared using either TruSeq Stranded mRNA Sample Library Preparation Kit (PolyA method) or Illumina Stranded Total RNA Prep with Ribo-Zero Plus (rRNA depletion method) (Illumina, San Diego, CA, USA).