GSM8818448: THP1_Macrophage_ARID5A_eCLIP_Input1; Homo sapiens; RIP-Seq
Cells were lysed and and sonicated in eCLIP lysis buffer, and RNA was fragmented using RNase I (Ambion) and complexes were immunoprecipitated with ARID5A-specific antibody from Atlas Antibodies RNA was reverse transcripted with Superscript III, and adaptor was ligated with T4 RNA Ligase 1, then libraries were constructed with Q5 High-Fidelity 2x Master Mix (NEB)