control
Shotgun metagenomic libraries were prepared  Illumina DNA Prep (M) Tagmentation protocol. Libraries were amplified with 5 PCR cycles and validated with fragment size distribution averaging 534 bp (range: 462-586 bp). Paired-end sequencing (2x151 bp) was performed on Illumina NovaSeq 6000 using S2 flow cell with 1% PhiX control.