GSM9149920: Dam-only in brain upon Lam-KD in neurons_rep2; Drosophila melanogaster; OTHER
The central brain was extracted in two replicates from approximately one hundred third instar male larvae of the corresponding genotypes. Several micrograms of genomic DNA were isolated from the larval brain. After that, PCR amplification of methylated GATC-GATC fragments of the genome was carried out according to (Vogel et al. 2007). PCR-amplified methylated fragments were digested with DpnII to remove adaptors and were purified using PCR purification kit (Qiagen). After that, libraries from the samples containing ~0.5 µg DNA were prepared for next generation sequencing on Illumina. Libraries were constructed according to standard protocol for sequencing on Illumina.