WGS of Escherichia coli strain: buriganga river water
From sample preparation, library construction to final data acquisition, every link may have an impact on the quantity and data quality of sequencing data, and data quality will directly affect the results of subsequent information analysis.Therefore, obtaining high-quality data is the premise to ensure correct, comprehensive and credible bioinformatics analysis.In order to ensure the accuracy and reliability of sequencing data from the source, Novogene strictly controls every production link to fundamentally ensure the output of high-quality data.The flow chart is as follows:1.1 Sample testing1.2 Library construction and sequencingAfter the DNA sample is qualified, it is randomly interrupted with a Covaris ultrasonic breaker, and then the entire library preparation is completed through the steps of end repair, A-tailing, sequencing adapter, PCR amplification, fragment screening and purification.to obtain the final DNA library.The library construction flow chart is as follows:Novogene's library detection mainly includes 2 methods:(1) AATI detects the integrity of library DNA fragments and the size of insert fragments.(2) QPCR detection library effective concentration.After the library inspection is qualified, different libraries are pooled according to the requirements of effective concentration and target off-machine data volume, and then paired-end sequencing is performed.