GSM8988959: Pooled heads from 13 wild-type zebrafish larvae - sample4; Danio rerio; RNA-Seq
Total RNA was extracted from samples using the RNeasy Mini Kit, Qiagen (Qiagen, Catalog No.NC9677589), following the manufacturer's instructions and including an on-column DNase treatment.  An additional ethanol precipitation was performed for extra purification. All samples had an RNA integrity number (RIN) score ranging from 9.3 to 9.5. Libraries were prepared using the TruSeq Stranded mRNA protocol. A 9 bp of Unique Molecular Identifiers (UMIs) tags were add added before PCR amplification to reduce errors and quantitative bias introduced by the amplification. All samples passed quality control (QC) and pre-processing, and were used for further analyses