GSM9197835: cuttag_Smad4_Smad4WT_replicate2; Homo sapiens; OTHER
Minute ™  Cytodynamic and Nuclear Extraction Kits for Cells were used to extract cell nuclei from mouse liver tissue. Cell nuclei were resuspended in the Wash Buffer, and after counting, 100K cells were added to each sample for CUT&Tag experiment. The CUT&Tag library was constructed using the reagent kit from Hefei STM Biotechnology Co., Ltd.. Libraries were amplified using 18 cycles on the thermocycler.  Libraries were validated using the Agilent High Sensitivity DNA Kit.