GSM8385634: Anaerobic exponential phase rep3; Lactobacillus johnsonii; RNA-Seq
For the in vitro samples, protease treament followed by RNA isolation was performed as per the bacterial RNA isolation protocol from Qiagen Rneasy Kit. For the in vivo samples, Trizol/Rneasy hybrid protocol by Mauricio Rodriguez-Lanetty was followed. NEBNext Ultra II DNA library prep was used for cDNA library preperation. RNA-seq. Sequencing was done using Novoseq, paired-end sequencing, with a sequencing depth of 33M reads per sample for in vitro samples and 150M reads per sample for in vivo samples.