Plasmodium falciparum sporozoite transcriptional response to CD36
Plasmodium falciparum sporozoite transcriptional response to CD36
P. falciparum sporozoites isolated from the salivary glands of infected mosquitoes were activated at 37°C in DMEM/F-12 medium supplemented with 10% FBS, 10 µM CD36 peptide, or both, or kept at 4°C to prevent activation. Each sample contained approximately 250,000 to 500,000 sporozoites. After 1 hour, sporozoites were spun down for 3 minutes at 13,000 rpm and resuspended in TRIzol reagent (Thermo Fisher Scientific) for RNA extraction. RNA was obtained by chloroform extraction. Total RNA was treated with DNase and purified using the RNeasy MinElute Cleanup Kit (QIAGEN). Library preparation was performed by Novogene (Cambridge, UK) using poly(A) mRNA enrichment.