GSM9228282: Pancreatic islets, WT, mRNA; Mus musculus; OTHER
Single cell solutions were obtained from pancreatic islets isolated from 6-8 C57BL/6J males as previously described. Immune cells were sort-purified as live DAPI-Epcam-CD45+ cells with a FACS ARIA III cell sorter (BD Biosciences) using FACS Diva software (BD Biosciences) and lysed for mRNA extraction. Sorted cells were then centrifuged at 300 rcf for 5 minutes and resuspended in PBS containing 0.04% of Ultrapure BSA (Invitrogen Cat# AM2616). Isolated cells were then stained with DNA barcoded antibodies (CD9, CD206) TotalSeq-B antibodies.