GSM9666799: 1-month, Prefrontal Cortex, heterozygous replicate 6; Mus musculus; RNA-Seq
Brain dissection was performed in the cryostat using frozen brain tissues collected from Srrm2 +/- mutant mice and wild-type littermates.Nuclei were purified by dissociating dissected brain tissue in a 1% Triton-X based buffer, followed by FACS enrichment. Libraries were prepared using 10X Genomics v3 (Single Cell 3') kit, using manufacturer's instructions. cDNA fragment sizes were analyzed using High Sensitivity DNA chips (Agilent), a 10 nM normalized library was pooled, and sequencing performed on a NovaSeq S2 (Illumina) with 28 and 75 bases for reads 1 and 2 and 10 bases each for index reads 1 and 2.