KM mouse hippocampus mRNA-seq (Model (D-gal))
Total RNA was extracted from mouse hippocampus using MJZol Total RNA Extraction Kit (Meiji Bio, Shanghai, China) and quantified with NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA). Eukaryotic mRNA was enriched from total RNA by poly(A) selection using oligo(dT) magnetic beads, fragmented to ~300 bp, reverse-transcribed to cDNA, ligated with adapters, size-selected and PCR-enriched. Libraries were sequenced on an Illumina NovaSeq X Plus using Illumina NovaSeq Reagent Kit (standard).