T. molitor transcriptome-PBS R3
Trizol regent was used to isolate total RNA. mRNA was purified from 1 g of total RNA and then fragmented  first. Random hexamer primers were used to synthesize double-stranded cDNA. After end-repairing, A-tailing and sequencing adapters connecting, 370-420 bp cDNA was screened using magnetic beads, followed by PCR amplification for 10-15 cycles and purification to obtain the final library.