SiLRL1-OX-9-2 (CRX2017327; CRA030335)
Total RNA was extracted from immature fruit pods of Arabidopsis thaliana (with three biological replicates per line) using the RNApure Plant Kit (DNase I) (CWBIO, Jiangsu, China). cDNA library construction was conducted by Biomarker Biotechnology Company (Beijing, China). Sequencing was carried out on the Illumina NovaSeq 6000 platform. Raw reads were processed to remove adapter sequences, reads containing ambiguous bases (N), and low-quality reads. The quality of the clean data was assessed by calculating Q20, Q30 scores, and GC content. Libraries were pooled according to effective concentration and target data volume for subsequent Illumina sequencing. After quality control, clean reads were aligned to the reference genome (Arabidopsis_thaliana.TAIR10.1.genome.fa) using HISAT2 (v2.2.6). Transcriptome assembly and reconstruction were performed with StringTie (v1.3.6) .