WXS of KKU-213A cell line:Human:RMC7977 resistant:6 weeks
Genomic DNA was subjected to quantity and quality control using Qubit dsDNA BR Assay Kit (Thermo Fisher Scientific) and 4200 Tapestation with Genomic DNA ScreenTape (Agilent Technologies) and used to prepare whole-exome sequencing (WES) libraries using the Twist Comprehensive Exome Panel for human samples (Twist Bioscience) and the Twist Mouse Exome panel for murine samples, following the manufacturers instructions. A total of 50 ng of fragmented (300-400 bp) DNA input was used for WES. Sequencing libraries were pooled and sequenced with a NextSeq2000 (Illumina) using paired-end runs (100 bp x2)