GSM8565863: CROP-seq,786-O,CRISPRa,scRNA; Homo sapiens; RNA-Seq
The cells were further processed using the Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (Dual Index) (10x Genomics) according to the manufacturer's protocol. To enrich for sgRNA transcripts, PCR was performed on 10 ng of cDNA from the 3' single-cell RNA libraries using specific primers. The libraries were sequenced on Illumina (Novogene), using a configuration identical to that of standard 10x Genomics libraries.