GSM9128170: Mouse tumor, EZH2knockout, biol rep5; Mus musculus; RNA-Seq
Total RNA from flash-frozen tumor pieces or cell pellets was extracted and purified using the FavorPrep Tissue Total RNA Mini Kit (Favorgen, FATRK001) according to manufacturer's protocol. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.