GSM8709689: PARP1, vector, 50μM etoposide, rep2; Homo sapiens; OTHER
Cells were treated with Con-A beads, antibodies and reagents following the instrunction of Hyperactive In-Situ ChIP Library Prep Kit. Libraries were prepared using the Hyperactive In-Situ ChIP Library Prep Kit for Illumina from Vazyme biotech co.. Two hundred thousand cells were used as starting material for samples. Library were amplified using appropriate cycles on the thermocycler. Post amplification libraries were size selected at 200-500bp in length using VAHTS DNA Clean Beads from Vazyme biotech co.. Libraries were validated using Qubit, AATI and QPCR.