GSM8785532: spleen Multiome, CON, GEX; Mus musculus; RNA-Seq
We isolated nuclei from 4 cryopreserved spleen cell samples and constructed single nuclei gene expression libraries and single nuclei ATAC libraries on the 10x Genomics Chromium System using the 10x Chromium Multiome kit. Each library was sequenced with ~200 million pair end reads (PE150) on Illumina NovaSeq. All data was generated using the 10x Chromium Single Cell Multiome platform. For each sample, the analysis input is the Cell Ranger ARC output, then we applied Seurat v5.1.0 (Hao et al., 2024) and Signac v 1.13.0 (Stuart et al., 2021) to create a multi-omic Seurat object with paired gene expression and DNA accessibility profiles for each sample.