GSM9097320: PSP23-HS5; Homo sapiens; OTHER
Primary motor cortex FFPE tissue blocks from 4 PSP patients with DV200 > 50% were selected for sectioning. Appropriate-sized sections were placed within the frames of capture areas on the Visium Spatial Gene Expression Slide (PN-1000188, 10x Genomics) with one section in each capture area (6.5 × 6.5 mm). Tissues were deparaffinized, stained and decross-linked, followed by probe hybridization, ligation, release, and extension. Visium spatial gene expression FFPE libraries were constructed with a Visium Human Transcriptome Probe kit (PN-1000363, 10x Genomics) and Visium FFPE Reagent kit (PN-1000361, 10x Genomics) following the manufacturer's guidance and sequenced on the Illumina NovaSeq 6000 platforms to achieve a depth of at least 25,000 mean read pairs. 10X Visium FFPE Probe-based library construction