GSM9843372: PeCa60_patient_tissue_sample; Homo sapiens; RNA-Seq
DNA and total RNA was isolated from patient tissue samples, matched primary cell cultures and first-generation xenograft tumors using RNeasy, RNeasy FFPE, QIAamp DNA and QIAamp DNA FFPE kits (Qiagen, Hilden, Germany) according to the manufacturer's instructions. Concentration and purity of isolated DNA and RNA were analyzed with a Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific, Schwerte, Germany). RNA-seq was performed as 3´-mRNA sequencing in a NovaSeq6000 sequencing system (Illumina, San Diego, CA, USA) with a QuantiSeq 3´-mRNA Library Prep (Lexogen, Vienna, Austria) and a 10M raw read coverage using 500 ng of highly pure RNA (A260 / A280 > 1.8, A260 / A230 > 2.0). RNA-libraries for RNA-seq were prepared using a QuantiSeq 3´-mRNA Library Prep (Lexogen, Vienna, Austria) according to the manufacturers instructions.