Arundinella anomala Genome sequencing and assembly
For PacBio sequencing, SMRTbell libraries were prepared from 8 g of genomic DNA per sample following the manufacturer's 15 kb protocol. Key steps included: DNA shearing using g-TUBEs (Covaris); DNA damage repair, end repair, and A-tailing; Ligation with SMRTbell adapters (SMRTbell Express Template Prep Kit 2.0); Nuclease treatment (SMRTbell Enzyme Cleanup Kit); Size selection using PippinHT (Sage Science); Polymerase binding. Library quality was verified using an Agilent 2100 Bioanalyzer before sequencing on a PacBio Sequel II system with Sequencing Primer V5 and Sequel II Binding Kit 2.2 at GrandOmics Biosciences Co., Ltd. A total of 67.3 Gb of PacBio high-fidelity (HiFi) circular consensus sequencing (CCS) dataapproximately 33 coverage of the estimated genome sizewas generated and subsequently used for de novo genome assembly. The assembly was performed using Hifiasm (version 0.16.0; (Cheng et al., 2021)), a haplotype-resolved assembler optimized for HiFi reads, with default parameters to generate high-contiguity contigs.