GSM9726434: Mk2i_1.fq.gz; Bacteroides thetaiotaomicron; RNA-Seq
Total RNA was purified from bacterial pellets using the miRNeasy Mini kit (Qiagen) according to the protocol #3 described in (Popella, Jung, Popova, Durica-Mitic, et al., 2021). Briefly, cells were resuspended in 0.5 mg/mL lysozyme (Roth) in TE buffer (pH 8.0) and incubated for 5 min. Afterwards, RLT buffer supplemented with β-mercaptoethanol, and ethanol were added according to the manufacturer's instructions. After sample loading, column wash-steps were performed according to the manual. RNA concentration was measured with a NanoDrop spectrophotometer. Libraries were prepared with the NEBNext Multiplex Small RNA Library Prep kit for Illumina according to the manufacturer's instructions.