GSM9197496: HCC95,rep2(input); Homo sapiens; OTHER
Genomic DNA was extracted from isogenic HCC95-NC and HCC95-KO-PTPRZ1 cell lines using the TIANamp Genomic DNA Kit (TIANGEN) , with quality verified by Quibt4 and electrophoresis. Genomic DNA were sonicated to generate chromatin fragment on a Covaris M220 Focused-ultrasonicator (Insert, microTUBE 130 µL; Temperature, 7°C; Peak Incident Power, 50W; Duty Factor, 20%; Treatment Time, 65s). Libraries were prepared from 4 μg fragmented DNA (~350 bp) with the Illumina TruSeq DNA PCR-Free Kit and sequenced on NovaSeq X Plus (PE150, 30× coverage). DNA was isolated by performing a phenol/chloroform/isoamyl alcohol extraction to prepare the WGS library using the NEBNext Ultra II DNA Library Prep Kit for Illumi-na (NEB, E7645).