amplicon sequencing of bacteria from a synthetic community
The ZymoBIOMICS Microbial Community DNA Standard II (Log Distribution) was used for PCR in duplicate with 335f/769r primers to generate 16S amplicons. Duplicate PCR reactions were pooled after agarose gel electrophoresis confirmed successful PCR amplification for both replicates. Amplicons were sent to the Cornell BRC for sequencing with a v2 500 bp kit with 2x250 Paired Ends on the Illumina Miseq.