RNA-seq of mouse liver
Two-month-old female mice were induced to estropause using 4-vinylcyclohexene diepoxide (VCD). At six months of age VCD-treated mice were divided in control group (VCD) and exosome group (EXO), with the latter receiving 10 injections of exosomes at 3-day intervals. Cyclic mice of the same age were used as controls (CTL). Exosomes were extracted from the serum of untreated cyclic mice of the same age and prepared for injection. RNA was extracted from liver for RNA sequencing. Exosomes were extracted from serum for RNA extraction and miRNA sequecing.