RNA-Seq of Schmidtea mediterranea strain S2F18: whole animal, ophis RNAi
Total RNA was extracted from animals lysed in TRIzol reagent (Invitrogen) using the Direct-zol RNA MiniPrep Plus Kit (Zymo Research, Cat. No. R20700), following the manufacturers protocol. For library preparation, 500 ng of poly(A)-selected RNA was used with the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs), using 0.6 M NEB adaptor and 12 PCR cycles.