GSM9724254: PC3 SLK-KO cells + MYC, replicate A; Homo sapiens; RNA-Seq
Cells were harvested with trypsin, washed with PBS, counted, and 5x10^5 viable cells per biological replicate were pelleted and resuspended in 100μL of 1X Zymo DNA/RNA shield (Zymo, #R1100-50). Samples were shipped to Plasmidsaurus at room temperature. Total RNA is isolated using a bead-based extraction approach. mRNA is converted into complementary DNA (cDNA) via reverse transcription and second-strand synthesis using a poly(dT)VN primer, followed by tagmentation, library indexing, and amplification. Sequencing is single end (~90bp reads) and 3' end counting is used to capture differential gene expression.