16s sequence of microorganismSargassum3
The V3V4 hypervariable region of bacterial 16S rRNA genes was amplified with primers 338F and 806R. The PCR products were purified with DNA magnetic beads (BGI, LB00V60) to remove primer dimers and non-specific amplification. Purified amplicons were subjected to paired-end sequencing (2  300 bp) on the DNBSEQ-G400 platform (BGI-Shenzhen, China).