CUT&RUN of Labroides dimidiatus adult forebrain (H3K27ac)
CUT&RUN experiments were performed on adult Labroides dimidiatus brain tissue following the Cell Signaling Technology CUT&RUN protocol (#86652) using H3K27ac antibody. Input DNA was processed according to Cell Signaling guidelines for Input DNA by MNase digestion. Library preparation and Illumina sequencing were performed by the Genomics Core (Centre for PanorOmic Sciences, HKU) using the KAPA Hyper Prep Kit (KR0961) with xGen Dual Index UMI Adapters. To optimize yield and quality, the post-ligation cleanup bead ratio was increased from 0.8 to 1.1. Libraries were validated by Agilent Fragment Analyzer, Qubit and qPCR, and sequenced on an Illumina NovaSeq 6000 platform (paired-end 151 bp). An average of 93% bases reached Q30 quality.