Arginine kinase of Culex quinquefasciatus sample NIMS2301664 from Diversity, phylogeny, and disease status of medically important arthropods on Diego Garcia Atoll
Mosquitoes were homogenized using a BeadBlaster 96 (Benchmark Scientific) and total nucleic acid was extracted using the Qiagen DNeasy Blood & Tissue kit. Arginine kinase amplicons were generated from genomic target using Q5 High-Fidelity 2X Master Mix. Amplicon libraries were made using the Illumina DNA Prep kit. Libraries were sequenced by the Navy Entomology Center of Excellence on a MiSeq using a v3 Reagent Kit at 251bp x 251bp.