GSM9664314: ET1; Homo sapiens; RNA-Seq
CD34+ and CD14+ cells were sequentially isolated using Miltenyi magnetic beads via positive selection, counted, and mixed into a single-cell suspension. The cells were then loaded onto a 10x Genomics Chromium Controller for GEM (Gel Bead-in-Emulsion) generation. GEM generation, cell barcoding, and cDNA library preparation were performed using the Chromium Next GEM Single Cell 3′ Reagent v3.1 (Dual Index) kit (10x Genomics) following the manufacturer's protocol.