Whole genome sequence of a colonic crypt from a HNRNPA1 Ctrl mouse
Individual colonic crypts were identified, demarcated and isolated by laser capture microdissection using a Leica Microsystems LMD 7000 microscope followed by lysis using the Arcturus Picopure DNA Extraction kit (ThermoFisher). Enzymatic fragmentation-based library preparation was performed on 110 ng of colony DNA, as previously described in Ellis et al. (2021) Nature Protocols 16:841.