WGS of Escherichia virus T4: independently evolved population under low osmolality
Whole-genome sequening was performed on phage populations from an evolution experiment in different osmotic conditions. Genomic DNA was extracted directly from refrigerated phage lysates using the Phage DNA Isolation Kit (Norgen) according to the manufacturer's instructions. Library preparation and sequencing were performed by the Sequencing and Bioinformatics Consortium at The University of British Columbia. Extracted DNA was quantified using Qubit fluorometry. Genomic libraries were prepared using the Illumina DNA Prep kit (Illumina). Samples were sequenced with the Illumina NextSeq 2000 sequencing platform generating paired end 150 bp reads.