Streptococcus periodonticum CRC221
Genomic DNA (gDNA) was processed in parallel: one portion was randomly fragmented to construct a300-bp insert library for paired-end 150-bp sequencing on the Illumina NovaSeq 6000 (Illumina, USA),while the other was sheared using g-TUBEs (Covaris, USA) to generate ~ 20-kb fragments. The Illuminalibrary was prepared using the NEBNext Ultra II DNA Library Prep Kit (NEB, USA) according to themanufacturers instructions. The PacBio library was constructed using the SMRTbell Express Template PrepKit 2.0 (PacBio, USA). After purification with AMPure PB beads (Pacific Biosciences, USA) and qualityassessment by 0.7% agarose gel electrophoresis, qualified samples were sequenced on the PacBio Sequel IIplatform using the Sequel II Binding Kit 2.0 and Sequel II SMRT Cells 8M, with a 10-hour movie time.Assembly of PacBio/Nanopore long-read data was performed using Canu (v2.2) and Unicycler (v0.5.0) withdefault parameters.