RNA-seq experiment of human microglia cell line comparing wild-type control to FAM49B-knockout
RNA-seq experiment of human microglia cell line comparing wild-type control to FAM49B-knockout
human microglia cell line HMC3 was used. After the completion of CRISPR/Cas9 KO of FAM49B,  total RNA was isolated from cell culture To isolate and purify the RNA, RNeasy plus (mini), Qiagen kit was used according to the manufacturer's protocol. The library preparation was performed by using an Illumina seq library kit (performed by Azenta)