GSM9421841: RMC6236 CD45+ scRNA-seq; Mus musculus; RNA-Seq
Tumor xenografts were dissociated into single-cell suspensions through combined mechanical disruption and enzymatic digestion in RPMI-1640 supplemented with 10% FBS, collagenase IV (1 mg/mL), DNase I (20 μg/mL), and hyaluronidase (100 μg/mL). Following digestion, cell suspensions were filtered and washed, and intratumoral CD45⁺ T cells were enriched by fluorescence-activated cell sorting (FACS; BD FACSAria III). Sorted cells were resuspended in PBS containing 0.2% FBS, and cell concentrations were adjusted according to the manufacturer's recommendations for the Chromium Single Cell 3' v3.5 platform (10× Genomics). Libraries were sequenced on an Illumina NovaSeq 6000 platform (Genergy Biotechnology) with a depth of ~90,000 reads per cell.