GSM9634143: VcaP cells, siCDO1A biol rep  2; Homo sapiens; RNA-Seq
RNA were harvested using miniRNeasy kit (Qiagen). RNA library were performed by novogene : ). Messenger RNA was purified from total RNA using poly T oligo attached magnetic beads, fragmented, and converted into first strand cDNA using random hexamer primers, followed by second strand synthesis incorporating dUTP to generate strand specific (directional) libraries. Libraries underwent end repair, A tailing, adapter ligation, size selection, amplification and purification, and were assessed by Qubit and real time PCR for quantification, together with electrophoretic evaluation of fragment size distribution