GSM9198783: AdxDHEA_15; Acomys dimidiatus; RNA-Seq
Tissues were collected into TRIzol Reagent (Life Technologies), homogenized and kept at -80°C until RNA extraction. The RNA fractions were isolated from each sample by phase separation, and RNA extracted from the upper phase with RNA Clean & Concentrator kit with DNase treatment (Zymo R1014) according to the manufacturer's protocol. RNA concentrations were determined using Qubit Fluorometer kits (broad range or high sensitivity, Invitrogen), according to the manufacturer's protocol, and RNA integrity measured using Agilent 2200 TapeStation. Reverse transcription was performed using Quanta-bio qScript cDNA synthesis kit (Quantabio 95047-100) according to the manufacturer's instructions, with 0.1-1 μg of RNA. NEBNext Ultra-Express RNA library prep