GSM9455765: H3K27ac_young_sirt6_tg_1; Mus musculus; ChIP-Seq
Trizol extraction (Phenol/chlorophorm) Chip-seq libraries were prepared at the Crown Genomics institute of the Nancy and Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science starting from 1-20ng of DNA as previously described (Blecher-Gonen R et al. Nat. prot. (2013)).Libraries were quantified by Qubit (Thermo fisher scientific) and TapeStation (Agilent). Sequencing was done on a Novaseq X Plus instrument (Illumina) using a Novaseq X 1.5B 100 cycles kit, allocating 20M reads per sample (paired end sequencing for H3K9ac & H3K56ac and single end for H3K27ac).