RNA-Seq of RS300
Genomic DNA of rumen solids was extracted using Quick-DNA ZR Fecal/Soil DNA kits including a 2 min bead-beating step at 1750 strokes per minute using Geno/Grinder 2010, The PCR was targeted to amplify the V3-V4 regions of the bacterial 16S rRNA genes using modified F338/R306 primers.