GSM9844038: MCF7, day21Palbo, rep1, RNA; Homo sapiens; RNA-Seq
RNA extraction was done using the Quick-RNA Microprep Kit (Zymo Research, cat# R1050). Total RNA from at least 2 biological replicates per condition was sent to Novogene for stranded library preparation and sequencing. cDNA libraries were made with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NEB cat# E7765). For MCF7 samples treated with  MCF7 cells were treated with 100 nM doxorubicin, 1 uM palbociclib or 100 nM palbociclib + 10 nM fulvestrant, samples were sent to PlasmidSaurus for forward-stranded library preparation. Plasmidsaurus'  RNA-seq service sequences polyadenylated (poly-A) eukaryotic transcripts using a 3′ end-counting approach to provide gene-level counts only, where a short stretch of RNA (~90nt) located within the last 300-400nt of the transcript is sequenced.