GSM9369830: GvHD_4plex_scRNAseq; Mus musculus; RNA-Seq
Donor-derived T cells were isolated from acute GvHD recipient mice, stained, and sorted by flow cytometry. Single-cell suspensions were prepared according to 10x Genomics recommendations. Single-cell 5′ gene expression libraries were generated using the 10x Genomics GEM-X Universal 5′ Gene Expression v3 chemistry with 4-plex on-chip multiplexing. GEM generation, reverse transcription, cDNA amplification, and library construction were performed following the manufacturer's protocol. Indexed libraries were sequenced on an Illumina NextSeq 2000 using a 100-bp paired-end configuration.