mock 1 community
Puregene kit was used for DNA extraction for Escherichia coli. QIAamp PowerFecal Pro kit was used for extracting DNA from Lactobacillus acidophilus and Bifidobacterium gallinarum. The DNA ratio in mock 1 was L. acidophilus: E. coli: B. gallinarum = 1: 1: 1. The Nanopore Native Barcoding Kit SQK-NBD114.96 was used for sequencing. Basecalling was performed using Dorado v0.8.0 under super accurate basecalling model.