GSM8625328: IR_6; Mus musculus; ATAC-seq
Nuclei were isolated from HSC with lysis buffer (10mM Tris-HCl, 10mM NaCl, 3mM MgCl2, 0.1% Igepal) and resuspended in Tn5 transposase mixture (Nextera Tn5 Transposase, 1xTagment DNA buffer (Illumina), and 0.2mg/ml digitonin (Promega). The tagmentation reaction was carried out for 30 minutes at 37°C followed by DNA purification using the MinElute Reaction Cleanup kit (Qiagen). Tagmented DNA was amplified with NEBNext High-Fidelity PCR Master Mix (NEB) and sequencing primers Ad1_noMX and Ad2.1-6 indexing primers. Libraries were cleaned and size-selected using Spriselect beads (Beckman)