KAS-seq of Barrett's oesophagus & OAC patients
KAS-seq of Barrett's oesophagus & OAC patients
The biopsies were collected from routine endoscopies. nuclei from patient samples nucleic acid extraction for KAS-seq was performed as described in Wu T, Lyu R, You Q, He C. Kethoxal-assisted single-stranded DNA sequencing captures global transcription dynamics and enhancer activity in situ. Nat Methods. 2020 17:515â€“523. Libraries were constructed by amplifying transposed DNA with Illumina Nextera adapters.