Oxford Nanopore Direct RNA-Sequencing of Drosophila melanogaster DSRP strain A4: whole body females
Total RNA was extracted from 7-day-old flies using TRIzol and reverse transcribed with the Induro Reverse Transcriptase kit. Libraries were prepared using the Oxford Nanopore Direct RNA Sequencing kit (SQK-RNA004) and sequenced on a PromethION RNA flow cell for 72 hours.