GSM9557215: RH iAP2XI-3, HA, rep3; Toxoplasma; OTHER
CUT & Tag was performed using the Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro following the manufacturer's protocol (Vazyme Biotech). RH iAP2Ⅺ-3-mAID-3HA strain was bound to Concanavalin A-coated beads and incubated with an anti-HA tag antibody (ab9110, Abcam). After primary and secondary antibody incubation, transposon activation and tagmentation were performed. Targeted DNA was isolated, amplified, and purified using VAHTS DNA Clean Beads (Vazyme Biotech).