Transcriptional Subtypes on Immune Microenvironment and Predicting Postoperative Recurrence and Metastasis in Pheochromocytoma and Paraganglioma
Transcriptional Subtypes on Immune Microenvironment and Predicting Postoperative Recurrence and Metastasis in Pheochromocytoma and Paraganglioma
Tumor tissue samples were obtained during surgical resection and immediately preserved in RNAlater or snap-frozen in liquid nitrogen. Samples were stored at -80°C until RNA extraction. Total RNA was extracted using TRIzol reagent (Thermo Fisher) or equivalent according to the manufacturer's protocol. RNA concentration and purity were measured using NanoDrop, and RNA integrity was assessed using Agilent Bioanalyzer. Poly(A)+ mRNA was enriched from total RNA and used to prepare cDNA libraries using a stranded mRNA library preparation protocol according to the manufacturer's instructions (BGI Genomics, Shenzhen, China).