Didymella segeticola treated with PCN for 36 hours, with a final concentration of EC70
The mycelium plug (diameter 4 mm) at the edge of CGMCC3.20152 colony 7d was transferred into PDB medium with final concentrations of EC70, and inoculated in an incubator at 25 for 36h. The RNA of the total sample was isolated and purified by TRIzol (thermofisher, 15596018) according to the operation scheme provided by the manufacturer. Then the quantity and purity of total RNA was controlled by NanoDrop ND-1000 (NanoDrop, Wilmington, DE, USA) and the integrity of RNA was detected by Bioanalyzer 2100 (Agilent, CA, USA). The concentration was >50ng/L, RIN value >7.0, and total RNA>4g.