GSM9777082: iDC-Ctrl1, Input; Homo sapiens; RNA-Seq
The infected or mock-infected cells was collected at 16 h post-infection (hpi) for total RNA extraction using TRIzol reagent according to the product manual. The total RNA was subjected to immunoprecipitation using the GenSeq® m6A MeRIP Kit (GenSeq Inc.), following the manufacturer's instructions. Briefly, RNA was randomly fragmented to ~200 nt by RNA Fragmentation Reagents.  Protein A/G beads were coupled to the m6A antibody by rotating at room temperature for 1 h. The RNA fragments were incubated with the bead-linked antibodies and rotated at 4°C for 4 h. After incubation, the RNA/antibody complexes were washed several times, and then, the captured RNA was eluted from the complexes and purified. RNA libraries for IP and input samples were then constructed with GenSeq® Low Input Whole RNA Library Prep Kit (GenSeq, Inc.) by following the manufacturer's instructions. Libraries were qualified using Agilent 2100 bioanalyzer (Agilent) and then sequenced.