RNA-seq of the OE19 cell line
RNA-seq of the OE19 cell line
OE19 cells were grown in DMEM medium containing 10% foetal bovine serum at 37°C incubator with 5% CO2. Cells were transfected using non-target control siRNA for 48 hours, followed by second siRNA transfection. Total RNA was extracted using an RNeasy plus kit (Qiagen, 74134) with DNase treatment according to the manufacturer's protocol. RNA-seq libraries were constructed following the TruSeq Stranded mRNA sample preparation protocol (Illumina).