HiFI whole-genome sequencing of Schmidtea mediterranea CIW4
Genomic DNA was prepared using the Preparing HiFi Libraries from Low DNA Input Using SMRTbell Express Template Prep Kit 2.0 protocol. Briefly, all gDNA was sheared to 14 to 22 kb with the MegaRuptor device (Diagenode) and 12 to 18 g sheared gDNA was used for library preparation. Depending on the gDNA input amount and performance during library preparation, the PacBio libraries were either size selected for fragments either larger than 3 kb with Ampure beads or for fragments larger than 8 to 10 kb using the BluePippin device. The size-selected libraries were prepared for loading following the instructions generated by the SMRT Link software (PacBio, version 10) and the HiFi Reads application. The Sequel II Binding Kit 2.2 (PacBio, USA) was used to prepare the libraries for loading, using the Sequel II DNA Internal Control Complex 1.0 (PacBio). All libraries ran on SMRT Cells 8 M (PacBio) using the Sequel II Sequencing Kit 2.0 (PacBio) on the Sequel II Sequencer (PacBio).