GSM8712298: SUDHL4-DMSO-CART19-REP3; Homo sapiens; ATAC-seq
Nuclei from 80,000 sorted SU-DHL-4 cells from all the experimental conditions were isolated and were subjected to the transposition reaction using Tn5 transposase for 30 minutes at 37°C with 1000rp mixing. ATAC-seq libraries were prepared using the Active Motif ATAC-Seq kit following the manufacturer's protocol. The transposed DNA was purified using DNA Clean and Concentrator (Zymo) and fragments were barcoded with unique i5 and/or i7 indexed primers. Final libraries were double size selected using AMPure beads prior to sequencing. Sequencing was performed on an Illumina NextSeq 2000 instrument to produce 75-bp paired-end reads.