GSM8679189: BMAL1 ChIP (PerKO, CT20_replicate2); Mus musculus; ChIP-Seq
Liver nuclei isolation and generation of MNase-digested soluble chromatin extracts (according to Lichtsteiner et al. 1987 and Khan et al. 2020). The libraries were prepared with NEBNext UltraII DNA Library Prep Kit for Illumina sequencing, enriching for fragments from 100-150bp with the 0.9X beads. PE-150 sequencing was performed on Illumina NovaSeq6000 system using NovaSeqS4 flow cell. Approximately 30,000,000+ 150 bases paired-end reads were obtained for each replicate.