RNA-Seq of P.elegans, body segments 25-27
Total RNA was extracted from twelve homogenized annelid fragments using TRIzol reagent, followed by purification with the Direct-zol RNA Miniprep Kit. Directional cDNA libraries were constructed from 72 total RNA samples using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina. Library size distribution and concentration were assessed using a QIAxcel Advanced System and confirmed on an Agilent 2200 TapeStation System. Final libraries underwent sequencing on an Illumina NovaSeq 6000 platform.