GSM8818315: UTD T cells, No EP, D0, donor ND602, RNA-seq; Homo sapiens; RNA-Seq
Live, CD45+, GFP- cells were isolated via FACS and preserved in RNAprotect Cell Reagent with storage at -80C until processing. Total RNA was extracted from fresh frozen cell pellets using the Qiagen RNeasy Plus Universal Mini Kit. cDNA synthesis was conducted using the SMART-Seq HT kit (Takara). Indexed libraries were prepared with the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB).