beetroot-microcosms-16S
Cell pellets and remaining saliva samples were thawed on ice, and DNA was extracted using the Zymo Quick-DNA Fungal/Bacterial Miniprep Kit according to the manufacturers instructions.  Sequencing libraries were generated using the 16S Barcoding Kit 24 (Oxford Nanopore Technologies, Inc.), according to the manufacturers instructions.  The 42 samples were sequenced across 4 Flongle Flow Cells (R10.4.1; Oxford Nanopore Technologies, Inc.) on a MinION sequencer (Oxford Nanopore Technologies, Inc.).  24 and 18 samples (i.e., all 42 samples in total), respectively, were initially sequenced across two Flongle Flow Cells.