Ratshotgun
Shotgun metagenomic libraries were prepared using the Qiagen FX DNA Library Kit and sequenced on the Illumina NovaSeq X platform with a 2150 bp paired-end layout. High-throughput sequencing yielded >400 depth, and quality control was performed using FastQC and Trimmomatic. Clean reads were assembled with MEGAHIT and metaSPAdes for downstream metagenomic analysis.