GSM8819661: sh3_PLUS-KD_3; Homo sapiens; RNA-Seq
and RNA extracted using the RNeasy Mini kit (Qiagen) with sample homogenisation by QIAshredder (Qiagen) as per manufacturer's protocols. libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD with UDI (Lexogen) on a Bravo NGS Workstation Option B (Agilent Technologies). ). Single read 100bp sequencing was performed by loading 220pM pooled libraries on a NovaSeq 6000 sequencer (Illumina) with standard loading.