GSM8919257: TdTomato+ and TdTomato- cells (50:50) from Ptbp1 fl/fl;Aldh1l1+;tdT+/- 17 weeks old mouse brain; Mus musculus; RNA-Seq
Mouse brain cortices were collected 12 weeks after tamoxifen injection and subjected to single cell isolation. After cell sorting, the single cell suspension of tdT+ cells were approximately adjusted to 1000 cells/ul. To enrich the tdT+ cells, for each animal, the cells subjected to 10x procedure were mixed as 1:1 ratio of tdT+ and tdT- from the same animal. The procedures were carried out according to the manufacturer's instructions for Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1 (10x Genomics # 1000128).