RNA-Seq of chicken: control hen pectoral muscle 3
Total RNA was extracted from wattle/Pectoral muscle of healthy adult broiler chickens using TRIzol reagent. RNA integrity was assessed by Agilent 2100 Bioanalyzer. mRNA was enriched using oligo(dT) magnetic beads and fragmented. Stranded RNA-seq libraries were constructed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina. The libraries were sequenced on an Illumina NovaSeq 6000 platform, generating 150 bp paired-end reads.