Amplicon-Seq of H.sapiens: adult female colorectum derived organoid
Libraries were generated using the AmpliSeq CancerHotSpot Panel v2 for Illumina (Illumina, Inc., San Diego, CA, USA; Cat# 20019161), whichtargets 2,800 COSMIC mutations across 50 oncogenes and tumor suppressor genes.Sample indexing was performed using the AmpliSeq CD Indexes Set A for Illumina(Illumina, Inc., San Diego, CA, USA; Cat# 20019105).