WGS of soil metagenome: alpine wetland soil sample KZ-22
Total genomic DNA was extracted from 0.2 g of soil using the E.Z.N.A. Soil DNA Kit (Omega Bio-tek, Norcross, GA, USA). Metagenomic libraries were prepared using the NEXTFLEX Rapid DNA-Seq kit after genomic DNA was fragmented to an average insert size of approximately 350 bp with a Covaris M220 ultrasonicator. Paired-end 300 bp sequencing was performed on the Illumina NovaSeq X Plus platform using the NovaSeq X Series 25B Reagent Kit, with an average sequencing depth of 10 Gb.