GSM8679055: Smad3 KO LPS Replicate 2; Mus musculus; RNA-Seq
Bulk RNA was extracted from cultured macrophages using Qiagen Rnesay mini kit Library preparation was performed according to the manufacturer's instructions for the Nextera XT unstranded library kit. The libraries were then pooled and sequenced on a NextSeq 2000 sequencer (Illumina) in paired-end mode, 100nt read length.