GSM9557342: WT, bio rep 2; Mus musculus; RNA-Seq
the SLAM-LSK (CD150+, CD48-, lineage-, Sca1+, cKit+) population was sorted directly into TRIZOL-LS using FACS Aria III (BD Biosciences, San Jose, CA, USA). 500 ng total RNA is used for rRNA-depletion (NEB, E6310), followed by directional library synthesis (NEB, E7420)Poly-A purification: Poly-T column kit (NEB, E7490), followed by NEB kit (E7420)Low-input: For RNA less than 50ng, rRNA-depletion and library synthesis by Takara SMARTer kit (usually input RNA amount is not defined due to low RNA concentration)