Syntrophomonas wolfei subsp. saponavida
DNA was extracted using the Zymo D4081 Quick-DNA Magbead plus Kit per the manufacturers instructions (https://geneseesci.com). DNA was fragmented using using g-Tubes (Covaris, cat# 520079) in an Eppendorf 5424 centrifuge for 60 seconds at 3000g for a total of 3 spins according to the manufacturers protocol. The average size of the sheared DNA was then calculated using the genomic DNA Assay (TapeStation - Agilent Technologies) for construction of genomic libraries. Sheared DNA was then concentrated using AMPure PB beads according to the manufacturer's recommendations (PacBio - Multiplexed Microbial libraries Using SMRTbell Express Template Prep kit 2.0, #101-696-100 version 07 - July 2020). Library preparation was then performed according to the PacBio protocol.