ITS1 amplicon:agricultural soil
Total genomic DNA extraction was adapted from Yeates et al. (1998). PCR amplification of ITS 1 region of the fungal rRNA cluster was performed with primers ITS5-138 1737F (5 GGAAGTAAAAGTCGTAACAAGG 3) and ITS2-2043R (5 139 GCTGCGTTCTTCATCGATGC 3). Amplicon cleaning, library preparation and subsequent sequencing was conducted at Novogene (UK), using Illumina Sequencing NovaSeq technology (paired-end reads of 250 nt). Samples were analyzed in duplicate (50 K tags per sample).