GSM8482429: GATA6+/+ Day 2 IgG biol rep 2; Homo sapiens; OTHER
500,000 H1-GATA6+/+ cells were harvested approximately 52 hrs after starting the cardiac differentiation protocol and CUT&RUN was performed using the CUTANA ChIC/CUT&RUN Kit (Epicypher 14-1048) according to the manufacturer's protocol.  Anti-GATA6 (n=3 independent biological replicates) (Cell Signaling Technology 5851) and Rabbit Anti-IgG (n=2 independent biological replicates) negative control (Epicypher 13-0042) antibodies were used at a 1:50 dilution (~0.5g). The DNA libraries were prepared with approximately 5 ng of CUT&RUN enriched DNA using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs E7645) and NEBNextMultiplex Oligos for Illumina(96 Unique Dual Index Primer Pairs) (New England Biolabs E6440); 14 PCR cycles were used to amplify the adaptor ligated target DNA