NCI Pediatric Malignancy
Total RNA was extracted using Qiagen AllPrep DNA/RNA mini kits. Ribozero stranded RNA libraries were prepared and sequenced on an Illumina 2500 according to the manufactures protocol (Illumina, San Diego, CA). Sequencer-generated bcl files were converted to fastq files using the bcl2fastq tool in CASAVA (Illumina, San Diego, CA) suite.