Single cell RNA seq of human CD3+ splenocytes from humanized mice
Single cell RNA seq of human CD3+ splenocytes from humanized mice
Spleens harvested from mice and smashed to single cell suspension. The CD3+ Cell isolation done using Miltenyi kit. Single cells were isolated via the Single-Cell Capture and cDNA Synthesis with the BD Rhapsody Express Single-Cell Analysis System according to the manufacturerâ€™s recommendations (BD Biosciences, 23-22952(02)) using the BD Rhapsody Cartridge Kit (BD Biosciences, cat. no. 664887) and the BD Rhapsody cDNA Kit (BD Biosciences, cat. no. 633773). Whole transcriptome, sample tag, and TCR/BCR libraries were prepared using the BD Rhapsody Whole Transcriptome Analysis (WTA) Amplification Kit (BD Biosciences, cat. no. 633801) and the BD Rhapsody TCR/BCR Amplification Kit (BD Biosciences, cat. no. 665345) following the BD Rhapsody System TCR/BCR full length, mRNA WTA and Sample Tag Library Preparation Protocol (BD Biosciences, 23-24018(03)). 