NextSeq 550 sequencing
Formaldehyde-crosslinked anti-HuR RIP-seq library from HUVECs, biological replicate 1.
Formaldehyde-crosslinked (0.1%) HUVEC lysates were subjected to RNA immunoprecipitation with anti-HuR or control IgG using magnetic A/G beads. RNA was recovered from the immunoprecipitate and small RNA-seq libraries were prepared from ~5 ng recovered RNA using the BioLiqX Small RNA-Seq Kit (HB202A) with the CATL semi-ligation workflow and 12 pre-amplification cycles. Libraries were sequenced as single-end 75 nt reads on an Illumina NextSeq 550.