GSM8450589: liver tissue, Apoa1KO ApoeKO 22wks NLD sample 2; Mus musculus; RNA-Seq
Total RNA was isolated from mouse tissues using the NucleoSpin RNA extraction kit according to the manufacturer's instructions, with on-column DNA digestion. The quality of the mRNA was tested using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA) before RNA- seq; samples with RNA integrity number <7.0 were discarded. RNA samples were processed using the RNA-Seq Sample Prep kit from Illumina (Illumina, Inc, CA). Eight to 9 tagged libraries were loaded on one lane of an Illumina flowcell, and clusters were created using the Illumina Cluster Station (Illumina, Inc, CA).