GSM9777374: zygotes_onecell_multiome_rna_for_h3k4me1_batch1; Mus musculus; RNA-Seq
Zygotes were manually dispensed in 2µl of M2 medium with 0.2µl Dynabeads Myone Carboxylic (Fisher, 10563775) and kept on ice. After a 5 min centrifugation at 800g at 4°C, the supernatant was removed using the magnetic plate and replaced by 2µl of lysis buffer. Plates were then frozen at -80°C. Multiome OneCell CUT&Tag experiment was performed in two biological replicates, each corresponding to zygotes from one mouse. Combination of OneCell CUT&Tag with single-cell full-length transcriptomics, as described in (Moutaux, Schwager et al., 2025)