GSM9230371: Kidney, adult (3 months), H3K27ac, rep3; Mus musculus; OTHER
For native CUT&RUN nuclei were extracted using conventional methods. CUT&RUN libraries were prepared using the NEB Ultra™ II DNA Library Prep Kit for Illumina. Reactions were performed at half scale using between 10 ng and 30 ng of DNA  starting material. The amount of inut was kept consistent across a given histone modification.  Libraries were amplified using 14 cycles on the thermocycler. Post amplification libraries were size selected at 200-500 bp in length using SPRIselect beads from Beckman Coulter. Libraries were validated using tapestation.