PacBio Revio HiFi of gill from Meretrix taiwanica
The gill was sampled from Meretrix taiwnaica after cutting its adductor muscle, then extracted DNA with MagCore Genomic DNA Tissue kit. The extracted DNA was then purified and size selected in a range of 15 to 25 kb. Processed DNA underwent end repair and A-tailing, was ligated to SMRTbell adapters, treated with exonuclease, and used to generate a SMRTbell library. The library was further reduced 10kb fragments with marker S1 on 0.75% Agarose gel cassettes , then sequenced with PacBio Revio system.