GSM9777276: RGW plus D-galactose_2; Mus musculus; RNA-Seq
Total RNA was extracted from C2C12 myotubes using the FavorPrep Total RNA Extraction Mini Kit according to the manufacturer's instructions. RNA quality and quantity were assessed prior to library preparation. mRNA sequencing libraries were prepared from total RNA using the MGIEasy RNA Directional Library Prep Kit according to the manufacturer's instructions. Poly-A-containing mRNA was enriched, fragmented, reverse-transcribed, adapter-ligated, PCR-enriched, circularized, and converted into DNA nanoballs for sequencing on the DNBSEQ platform.