GSM9202401: DPCP day2 vs Control day0; Mus musculus; RNA-Seq
Full-thickness skin samples were harvested and subjected to Liberase TM digestion. Cell sorting was performed using the BD FACSAria™ III Sorter to isolate CD45+ cell populations. Single-cell RNA sequencing (scRNA-seq) of skin CD45+ immune cells was performed using the 10X Genomics Chromium 3' Solution platform. Libraries were sequenced on an Illumina NextSeq with paired-end sequencing.