GSM8679129: Ctrl1; Mus musculus; RNA-Seq
eGFP+ Treg cells were isolated from the liver of control and AIH mouse models using fluorescence-activated cell sorting (FACS). Single cells were lysed, and total RNA was reverse transcribed into cDNA using the SMART-SeqII protocol. The process included cDNA synthesis and pre-amplification to enable downstream library construction and sequencing. cDNA libraries were constructed following the SMART-SeqII protocol, utilizing a transposon-based method for fragmentation and adapter ligation. After cDNA quality control, the libraries were prepared for sequencing by generating DNA nanoballs (DNBs) through denaturation, circularization of single-stranded PCR products, and subsequent amplification. The final libraries were sequenced using combinatorial Probe-Anchor Synthesis (cPAS) technology, ensuring high precision and sensitivity for single-cell RNA quantification.