16S sequencing of human adult saliva
After collection, saliva samples were aliquoted following manufacturer instructions and stored at -80C until being transferred to the UCLA Microbiome Core facility on dry ice. DNA was extracted by bead beating using MAG MAX 96 well-plate kits paired with a Thermo KingFisher (Waltham, MA) according to the manufacturers instructions. The V4 region of the 16S rRNA gene was amplified using barcoded primers containing Illumina flow-cell compatible adapter sequences. The resulting amplicons were pooled in equal amounts to create the sequencing library, which was sequenced on an Illumina MiSeq using the 2 x 250 bp v2 kit.