GSM9100336: CK-2; Rattus norvegicus; RNA-Seq
Total RNA was isolated using Trizol reagent (Invitrogen) according to the user guide for RNA-seq analysis. Total RNA was extracted, and the eukaryotic mRNA was enriched by Oligo (dT) beads. Then the enriched mRNA was fragmented into short fragments using fragmentation buffer and reversely transcribed into cDNA by using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #7530, New England Biolabs).