GSM8821300: THP1_Macrophage_NegativeControl_IgG_Rep1; Homo sapiens; OTHER
Nuclei were isolated per Epicypher CUTANA CUT&RUN protocol and subjected to immobilization and permeabilization using Concanavalin-coated beads and complexes were immunoprecipitated using ARID5A specific antibody from Atlas Antibodies, and positive (H3K4Me) and negative (IgG) control antibodies from EpiCypher. pAG-MNAse is used to cleave target complex. Isolated DNA fragments are repaired to generate blunt ended DNA, and 5'end is phosphorylated and 3' end is labeled with dAMP (non-templated). Adapter is ligated and uracil is added to digest hairpin loops. DNA is purified, amplified by PCR and sequenced using EpiCypher CUT&RUN library prep kit.