GSM9558437: R161CBm_RNA; Macaca mulatta; RNA-Seq
Frozen cerebellar cortex tissue was homogenized and nuclei were isolated using detergent-based resuspension buffers containing NaCl, MgCl₂, Tris-HCl, NP-40, Tween-20, digitonin, protease inhibitors, DTT, BSA, and RNase inhibitor. Nuclei were filtered through 70 µm strainers, centrifuged at 500 × g at 4°C, and resuspended in nuclei buffer supplemented with DTT and RNase inhibitor. Nuclei concentration was adjusted to 4.5 × 10⁶ nuclei/mL prior to microfluidic capture. Single-nucleus RNA and chromatin accessibility libraries were prepared using the Chromium Next GEM Single Cell Multiome ATAC and Gene Expression kit (10x Genomics, PN-1000283) according to the manufacturer's instructions. Approximately 10,000 nuclei were targeted per sample using the Chromium microfluidic system with Multiome Gel Bead Kit A and Multiome GEM Kit A. RNA and ATAC libraries were generated in parallel from the same nuclei preparation.