GSM9341037: IEM_3C_HTGTS_WT_rep5; Mus musculus; OTHER
About 10 million cells were crosslinked with 2% formaldehyde and quenched with glycine. Then, the crosslinked cells were lysed in the 3C lysis buffer and nuclei were digested with NlaIII enzyme (R0125, NEB) at 37 ℃ overnight. The digested samples were ligated by T4 DNA ligase (M1801, NEB) at room temperature for at least 6 hrs, followed by de-crosslinked with Proteinase K (03115852001, Roche) at 56 ℃ overnight. The 3C templates were purified by phenol/chloroform and used for making 3C-HTGTS libraries with iEu baits. Libraries were prepared using 3C samples by HTGTS (Jane et al., 2018).