RNA-Seq of Homo sapiens: BMSCs encapsulated in GelMA hydrogel with HIF-1 induction
Primary human BMSCs were encapsulated within a 3D gelatin methacrylate (GelMA) hydrogel scaffold and treated with a chemical agent (e.g., deferoxamine) to stabilize HIF-1 expression. Total RNA was isolated and mRNA-Seq libraries were constructed via oligo-dT enrichment. Sequencing was performed on an Illumina NovaSeq 6000 instrument (Paired-end, 150bp). The objective is to identify differential gene expression involved in osteogenic-angiogenic coupling under scaffold-mediated hypoxia signaling.