Impact of MAREMO peptide MP5 on gene expression in tumor cells
Impact of MAREMO peptide MP5 on gene expression in tumor cells
Samples were collected using a cell scraper from cultured cells. NT193 cells were cultured for 18 hours in Dulbecco's modified Eagle's medium (DMEM Dutscher L0104-500) containing 4.5 g/L glucose, L-glutamine, sodium pyruvate and supplemented with 10% of heat-inactivated fetal bovine serum (FBS, Dutscher S1810-500), penicillin (100 U/mL), streptomycin (100 μg/mL) (Pan Biotech P06-07100), 2 mM Glutamax (Gibco 35050-061) and Gentamicin sulfate 40 μg/ml (Dutscher L0012- 100). NT193M cells were incubated with MP5 (50 microgr/ml) in 0.1% FCS/DMEM for 72 hours before collection of RNA and RNA seq analysis. The control condition was treated with 1X PBS. Total RNA was extracted using TriReagent (Life Technologies AM9738)  Sequencing libraries were prepared using the Smarter Stranded Total RNA-Seq kit v3 Pico Input Mammalian following the manufacturer's instructions (Takara Bio, San Jose, CA, USA)