shotgun seq
CeleroTM DNA-Seq Library Preparation kit (Tecan Genomics, Redwood City, CA) has been used for library preparation following the manufacturers instructions. Both input and final library were quantified by Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA) and quality tested by Agilent 2100 Bioanalyzer High Sensitivity DNA assay (Agilent technologies, Santa Clara, CA). Libraries were then prepared for sequencing and sequenced on NovaSeq X platform (Illumina, San Diego, CA) in paired-end 150 bp mode.