GSM8917424: KuCtrl, EtOH, 4days, rep1; Homo sapiens; RNA-Seq
RNA was isolated from cells using TRIzol (Invitrogen) following the manufacturer's protocol. RNA quality was determined using the TapeStation System (Agilent) or Bioanalyzer RNA kit (Agilent) and samples with RNA Integrity Number (RIN) higher than 9 were sent for next-generation sequencing (NGS). For some samples (descirbed as "AmpSeq" in the sample description), poly-A enrichment was used to enrich mRNAs from total RNA samples at day 1, 2, 4, and 6 after IAA&Dox induction, day 2 and 4 after CDK4/6 inhibitor and IAA&Dox induction, and day 4 after 4OHT treatment, then library construction was performed using Illumina TruSeq chemistry and sequenced at AmpSeq. For other samples (described as "Genome Center" in the sample description), ribo-depletion was used to enrich mRNA and non-coding RNA from total RNA samples both before and at 8 days after doxycycline withdrawal. The library construction was performed using Illumina TruSeq chemistry. The final PCR step has been modified using the KAPA HiFi HotStart Ready Mix.The libraries were then sequenced using Element AVITI at Columbia Genome Center.