RNA-Seq Glia Culture: Control 1
Total RNA was isolated from the collected cells with RNeasy Mini Kit (74104 Qiagen) using 1 million cells/sample. NGS library preparation was carried out using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760) with NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490). Finally, double-stranded cDNA was end-prepped and Illumina-specific adaptors were ligated to the cDNA fragments, followed by a final enrichment PCR.