IgG DiMeLo-seq of HG02769
DiMeLo-seq was performed as described in Altemose et al., 2022 with some modifications. Briefly, nuclei were isolated from cells and incubated with a rabbit anti-IgG antibody (ab171870, Abcam), washed, and then incubated with a mouse nanobody-Hia5 enzyme (Nicolas Altemose; Stanford University). The Hia5 enzyme was activated by the addition of S-adenosyl methionine (SAM; B9003S, NEB) for 2 hours, and then the DNA was isolated using the Monarch HMW DNA Extraction Kit for Cells & Blood (T3050S, NEB). DNA libraries were prepared using the Oxford Nanopore Technologies (ONT) SQK-ULK114 kit and sequenced on a PromethION 24 using R10.4.1 flow cells.