GSM9777753: Gsk3b (IEC+/+) ATAC-Seq 1; Mus musculus; ATAC-seq
Each colon cancer tissue sample (weighing about 500 mg) was finely ground and lysed. The nuclei were then isolated through centrifugation at 2000 * g for 5 minutes. Then, the TruePrep DNA Library Prep Kit V2 for Illumina (TD501; Vazyme Biotech) was utilized for transposition and high-throughput sequencing library preparation. Finally, these ATAC-Seq libraries were then enriched, quantified, and sequenced on a DNBSEQ-T7 platform (BGI, Shenzhen, China) using the PE150 strategy.