GSM8927493: NC_HA1; Homo sapiens; RNA-Seq
Extraction of total RNA following the manufacturer's instruction of the AllPrep DNA/ RNA/miRNA Universal Kit (80224, Qiagen, USA). For library preparation, 1 mg of total RNA from each sample was depleted for rRNA using the Ribo−Zero rRNA Removal Kit (RZH1046, EPICENTRE, USA), followed by construction of sequencing library using the TruSeq RNA Sample Prep Kit v2 (RS−122, Illumina, USA).