Transcriptome profiling of HEK293F cells and RIP-seq analysis of ZATT-interacting transcripts
Following UV-crosslinking (at 254 nm) anti-FLAG immunoprecipitation (four replicates per sample), stringent washing, and DNase treatment, RNA was eluted by proteinase K treatment and phenol-chloroform extraction. RNA-seq experiments were performed on three biological replicates per sample. After the RNA quality check by TapeStation, the sequencing library was prepared using Illumina Stranded total RNA Prep Ligation Protocol according to the manufacturers instruction, each sample was prepared with its unique barcode and sequenced at the NIEHS sequencing core.