GSM8625015: TSC1_TSC2_null_Rep2; Mus musculus; RNA-Seq
Single-cell RNA-seq was performed as described previously in Loo et al., 201949 and  Li et al, 201884. Briefly, P0 cerebral cortical neurons from control and TSC1/2 cKO mice were dissociated and stored in 80% methanol at -80°C. Cells were then lysed in TRI-reagent and RNA was extracted using a Direct-zol kit (Zymo Research Cat#R2051) with on-column DNase treatment, following the manufacturer's instructions. Strand-specific total RNA-seq libraries from ribosomal RNA-depleted RNA were prepared using the 10xG 3Prime v3 single-cell RNA kit (10X Genomics) according to the manufacturer's protocol. Libraries were sequenced single-end for a minimum of 76 bp for each index to a depth of approximately 40 million host genome reads on an Illumina NextSeq 500 instrument at the Yale Center for Genomic Analysis.