GSM9131360: S1(WT), S2(Satb1 F/F; ThPOK-Cre), S3((Satb1 F/F; E8I-Cre); Mus musculus; RNA-Seq
Tumor tissue was treated by BD Horizon Dri Tissue & Tumor Dissociation Reagent (BD Biosciences) and CD45+ cells were sorted (BD Symphony S6). Sorted cells were fixed by Chromium Next GEM Single Cell Fixed RNA Sample Preparation Kit (10x Genomics) and frozen at -80°C until ready. 1,000,000 cells were loaded onto Chromium X and 12,000-14,000 cells per sample were captured for the library preparation by Chromium Fixed RNA Kit - Mouse Transcriptome (10x Genomics).