RNA27
MODE-K murine intestinal epithelial cells were cultured in DMEM + 10% FBS and treated with 40 M ochratoxin A (OTA) for 0, 12,24,36, and 48 h, with DMSO as the vehicle control. Cell viability (CCK-8), intracellular ROS (DCFH-DA), and apoptosis were assessed. RNA-seq (Illumina NovaSeq 6000) was performed, and differential expression was analyzed using edgeR (FDR < 0.01), followed by GO&KEGG enrichment, MFUZZ time-series clustering, and qRT-PCR validation.