GSM9131170: LM2_ctc_cluster_m2; Homo sapiens; RNA-Seq
For all cell types, viability assessment and fluorescence-based sorting were performed using a BD FACSAria Fusion cell sorter. Dead cells were excluded using SYTOX blue dead cell stain. Cancer cells were identified and sorted based on fluorescent markers: GFP for LM2 cells, RFP for BR16 cells, and CD90.2 for MVT1 cells, with the latter stained using Alexa Fluor 488 anti-mouse CD90.2 antibody. Single-cell RNA sequencing libraries were generated using the 10x Genomics Chromium Single Cell 3′ Gene Expression Kit v3. Briefly, viable single-cell suspensions were loaded onto Chromium Single Cell Chips, and libraries were constructed following the manufacturer's protocol.