Truth set plasmid - pUNO with SARS-CoV2 Spike gene mixed plasmid library on R10.4.1 : 85% Omicron 10% Delta 5% WT
Plasmids digested with DpnI and library preparation with ONTs ligation sequencing kit SQT-LSK109 followed by barcoding with native barcode expansions 1-12 (EXP-NBD104)