GSM9422170: T47D-YA cells with the empty vector control 1; Homo sapiens; RNA-Seq
Total RNA was extracted using TriReagent. RNA concentration and integrity were measured on an Agilent 2100 Bioanalyzer (Agilent Technologies). RNA samples with RNA integrity number (RIN) over 5 were considered for RNA sequencing. mRNA from T47D-YA cells overexpressing -18 or -22.5 kDa FGF2 isoforms, and from empty vector controls (MOCK), was processed for directional library preparation with the TruSeq RNA Sample Preparation Kit v2 (Illumina). Paired-end sequencing (101-nt) was performed on an Illumina HiSeq2500 platform at Macrogen Core Facility, yielding ~30 million reads per sample.