KAS-seq of OE19 cell line
KAS-seq of OE19 cell line
OE19 cells were treated with DMSO or 500 nM lapatinib for 1 day or left untreated, and then trypsinised and collected. OE19 cells nucleic acid extraction for KAS-seq was performed as described in Wu T, Lyu R, You Q, He C. Kethoxal-assisted single-stranded DNA sequencing captures global transcription dynamics and enhancer activity in situ. Nat Methods. 2020 17:515â€“523. Libraries were constructed by amplifying transposed DNA with Illumina Nextera adapters.