GSM9372210: CUT&Tag-seq(JUN), GFP+ AT2 cells,CKO mice-2; Mus musculus; OTHER
GFP+ AT2 cells were sorted by flow cytometry from bleomycin-treated(8 days post-injury)  SftpcCreERT2; Rosa26mTmG(Control) or SftpcCreERT2; Rosa26mTmG; IPF/F(CKO) mice. the cells were mixed with 10 μL of ConA beads and incubated at room temperature for 10 min. The samples were then incubated with JUN primary antibody (#9165T;  Cell Signaling Technology) at 4 °C for 1 h, followed by incubation with goat anti-rabbit IgGat room temperature for 1 h. After incubation with the antibody, the samples were mixed with pAG-Tn5 transposomes and subjected to tagmentation for DNA extraction. CUT&Tag -seq libraries were prepared using the  using the CUT&Tag Assay Kit (#RK20265, Abclonal) .