Amplicon-NGS of HCV IRES: sample 996 (genotype 1a)
libraries were made with TruSeq Nano DNA LT Library prep kit (Illumina, San Diego, CA, USA), following the manufacturers instructions. IRES PCR products obtained by One-Step PCR and purified, were quantified with Qubit 2.0 Fluorometer dsDNA HS Assay (Life Technologies, Eugene, OR, USA) before use. A paired-end (2x250) sequencing run was performed with MiSeq Reagent Kit v2 (500 cycle) (Illumina, San Diego, CA, USA) on a MiSeq instrument (Illumina).