GSM8626780: granulosa cells, hCG, 3h, FOS_ChIPseq, rep1; Mus musculus; ChIP-Seq
Cells were fixed in fresh 11% formaldehyde for 15 minutes, then were quenched with 2.5 M glycine solution. Cells were then sent to ActiveMotif (Carlsbad, CA), were treated with cell lysis buffer, then sonicated with the EpiShear probe sonicator (Active Motif, 53051) ChIP-Seq libraries were prepared on an automated system (Apollo 342, Wafergen Biosystems/Takara)