GSM8436719: PGP1 DDX4-tdTomato/REC8-mGreenLantern, No TFs, hiPSC_naivereset; Homo sapiens; Bisulfite-Seq
Genomic DNA was extracted from hiPSCs, hPGCLCs, and sorted DDX4+ iOLCs using the Qiagen DNeasy Micro kit. Libraries were prepared from 200 ng DNA using the NEB Enzymatic Methyl-Seq kit (NEB #E7120) following the manufacturer's instructions. Briefly, control DNA (methylated pUC19 and unmethylated λ phage) was spiked into each sample. Next, samples were sheared to ~300 bp using a Covaris S2 sonicator. Enzymatic methyl conversion was performed, and libraries were amplified using unique dual index primers. 2 x 150 bp paired end sequencing was performed on an Illumina NovaSeq. Methyl-seq (NEB enzymatic kit)