Long ONT reads used for the de novo assembly of Klebsiella pneumoniae strain RSU-KP_ST14-1 genome
Total DNA was extracted from several suspended individual colonies of Klebsiella pneumoniae strain RSU-KP_ST14-1 using Proteinase K/SDS treatment and Genomic DNA Clean & Concentrator-10 Kit (Zymo Research). Library preparation was then generally carried out in accordance with the ONT Rapid Barcoding Kit 24 V14 (SQK-RBK114.24) Nanopore-only Microbial Isolate Sequencing Solution (NO-MISS) protocol, using an individual barcode prior to pooling with other barcoded samples. The resulting library was sequenced using the FLO-MIN114 flow cell (R10.4.1) on a MinION Mk1D device. Basecalling was performed using the dna_r10.4.1_e8.2_400bps_sup@v5.0.0 model and was followed by demultiplexing. Demultiplexed reads were then filtered using Filtlong (v0.2.1; --keep_percent 90 --min_length 500 --min_window_q 20) prior to upload here.