RNA-Seq of adult male tick
Sequencig library was performed using the Quick-16S NGS Library. The final pooled library was cleaned up with the Select-a-Size DNA Clean and quantified with TapeStation. Unique amplicon sequences were inferred from raw reads using the DADA2 pipeline, Chimeric sequences were also removed with the DADA2 pipeline. Taxonomy assignment was performed using Uclust from Qiime v.1.9.1