RNA-Seq of mouse: 14day female ovary
CUT&Tag libraries were constructed from 40 MII oocytes per sample using the Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro (Vazyme). Samples were incubated with primary antibody against Histone H3 (acetyl K9+K14+K18+K23+K27) (Abcam, ab47915), followed by pA/G-Tn5 mediated tagmentation and 35 cycles of PCR amplification for sequencing library preparation.