GSM8927812: SW620 cells, KO screen, T20 0.38µM oxaliplatin; Homo sapiens; OTHER
At least 1 × 10^9 cells were collected and genomic DNA was extracted using the QIAmp DNA blood Cell Maxi Kit (Qiagen). sgRNA sequences were amplified using Taq polymerase (Takara Bio, Inc.) and adapted for sequencing. The desired DNA product was purified with 6% TBE gel (Invitrogen) and subjected to massive parallel amplicon sequencing carried out by an Illumina sequencer (Sanger/Illumina 1.9).