GSM8770273: 5_RNASE1_sg3_A; Homo sapiens; RNA-Seq
RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser. The Illumina mRNASeq protocol involves Oligo(dT) magnetic beads to capture and purify RNAs with polyA tails. The RNA is fragmented into small pieces and the cleaved RNA fragments are copied into first strand cDNA using reverse transcriptase and random primers, followed by second strand cDNA synthesis using DNA Polymerase I and RNase H. The resulting double-strand cDNA is used as the input to a standard Illumina library prep with end-repair, adapter ligation and PCR amplification being performed to give you a library that is ready to go on the sequencer.