GSM8450403: db1, heart, ATAC-seq; Mus musculus; ATAC-seq
50,000 counted nuclei were then subjected to a Tn5 transposition reaction which was incubated at 37 ℃ for 30 min. The transposed DNA were purified with the MinElute PCR Purification Kit (Qiagen) and were amplified with the NEBNext High-Fidelity 1X PCR Master Mix (New England Biolabs, MA). The assays for transposase-accessible chromatin with high throughput sequencing (ATAC) assays were performed in Jiayin Biotechnology Ltd using standard Illumina protocols.