GSM9203374: U87 MG rep 1, m6A eCLIP; Homo sapiens; RIP-Seq
Total RNA was extracted from cells using the QIAGEN RNeasy Midi Kit, according to manufacturers instructions. Total RNA was sent to Eclipse Bioinnovations for processing, IP, library preparation, and analysis. Total RNA was processed as per EclipseBio protocols for m6A-eCLIP: polyA selection, fragmentation, m6A immunoprecipitation and crosslinking, input and IP processing, reverse transcription, and then library amplification.