GSM8991904: MCF10DCIS wildtype scramble mimic replicate 2; Homo sapiens; RNA-Seq
Total RNA from cultured cells was prepared using TRIzol reagent (Thermo-Fisher), followed by column clean-up using the Monarch RNA Cleanup kit according to the manufacturer's protocol for whole transcriptome isolation (New England Biolabs, T2030S). RNA quality was checked using an Agilent 2100 Bioanalyzer, and concentrations from ThermoFisher's NanoDrop readings were used. Stranded RNA-seq libraries were constructed using the NEBNext Poly (A) mRNA isolation kit, followed directly by the IDT XGen Broad Range RNA Library Prep Kit and the established protocols.  Library construction was performed using up to 1 µg of RNA. Each library was indexed during library construction for multiplex sequencing, and the quality and quantity were determined using Invitrogen's Qubit 4 fluorometer and an Agilent 2100 Bioanalyzer.