GSM9292652: Spleen, Tex_CITE-seq_r1_ADT; Mus musculus; OTHER
Cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq) was performed on WT and ID3KO GP33-tetramer+ CD8+ T cells, or CD45.2+ sgThy1 and sgId2 P14 CD8+ T cells sorted on day 8 post-infection with LCMV- clone 13. Construction of gene expression libraries and cell surface protein libraries was performed using the 10X Genomics Chromium Next GEM Single Cell 3' library kit (PN-1000269), following the manufacturer's instructions. The resulting gene expression and cell multiplexing libraries  were combined at a molecular ratio of 9:1. Libraries were quantified using a KAPA Library Quantification Kit (KK4835), assessed for quality using an Agilent Tapestation, and then sequenced on Illumina's NovaSeq X plus in paired-end read mode with the read length of 150 nucleotides at the Admera Health.