GSM8819973: day 1 adult, ev, BAY2402234 500 uM , batch1, rep1; Caenorhabditis elegans; RNA-Seq
Total RNA was extracted by adding 1 mL of TriPure Isolation Reagent (Cat. 11667165001, Roche) to each worm pellet. We then freeze-thawed the sample eight times (alternatively frozen in dry ice and then thawed in a 37°C water bath) in order to disrupt cell membranes.  RNA was then extracted using the Macherey-Nagel column-based RNA extraction kit (Cat. 740955.250) RNA sequencing was carried out by the Beijing Genomics Institute (BGI) on the BGISEQ-500 platform.