GSM9521826: iPSC-CM, Day 16, EV-miRNA, biol rep 2; Homo sapiens; miRNA-Seq
EV-derived miRNAs were extracted from conditioned media collected at each differentiation stage using the exoEasy Maxi Kit for Exosome Isolation (QIAGEN) according to the kit protocol. Library preparation was performed using a NEBNext Multiplex Small RNA Library Prep Set for Illumina kit (NEB) according to the manufacturer's instructions. The resulting libraries were size-selected by agarose gel electrophoresis and purified using a QIAquick Gel Extraction Kit (QIAGEN).