Adenocarcinoma_Baseline_19
Stool samples were self-collected at baseline (T0) and at first radiologic response (T1, ~8-12 weeks) using an mWRPAR microbiome collection tube. DNA was extracted using the Manta semi-automated magnetic-bead extractor, followed by sequencing on an ONT PromethION (P2i) instrument. Sequencing reads were basecalled, demultiplexed, and stored in FastQ format for further analysis.