Rock Canyon Lichen ITS2 metabarcoding
The ITS2 region was amplified using a polymerase chain reaction (PCR) with primers ITS3F (GCATCGATGAAGAACGCAGC) and ITS4R (TCCTCCGCTTATTGATATGC). PCR products were sequenced at RTL Genomics (Lubbock, TX), using 2x300 paired-end sequencing on the Illumina MiSeq platform.