Soil-associated fungi from 150 German grassland and 150 German forest plots from 2279
Sampling took place in May 2021 on all EPs of the Germany Biodiversity Exploratories. Along a transect, 14 soil cores of the upper 10 cm of soil were taken, compiled, sieved and stored at -20C. Amplification of ITS2 with fITS7 (GTGARTCATCGAATCTTTG) and ITS4 (TCCTCCGCTTATTGATATGC); nested amplification of SSU (18S gene) with primers GLOMERWT0 (AATARTCAATGCTCTATCCCA) and GLOMER1536 (CGAGDWTCATTCAAATTTCTGCCC), followed by NS31 (TTGGAGGGCAAGTCTGGTGCC) and AML2 (GAACCCAAACACTTTGGTTTCC).