GSM9777750: GSK3B wild-type RKO Input ChIP-Seq 1; Homo sapiens; ChIP-Seq
The chromatin immunoprecipitation (ChIP) protocol was performed to analyze histone modifications. Briefly, cells were collected and treated with 1% formaldehyde for 10 minutes at room temperature to induce crosslinking. After sonication, an anti-H3K27ac antibody was used for immunoprecipitation, and then the DNA was extracted and purified using the Universal DNA Purification Kit. The ChIP-seq library was prepared using the ChIP-Seq DNA sample preparation kit (NEBNext Ultra II DNA) according to the manufacturer's instructions. Then, these H3K27ac ChIP-Seq libraries were quantified using Qubit fluorometric assay (ThermoFisher) and sequenced on an Illumina Novaseq 6000 sequencer (Illumina, San Diego, California, United States) using the PE150 strategy.