GSM9523738: FACS sorted lung pooled from 3 mice_ CCR2KO_0Dpi_PBS_rep1; Mus musculus; RNA-Seq
Lungs were processed and were FACS sorted (100 000CD45+, 100 000CD45-, 10000Epi) into PBS-BSA, spun down and fixed in 10x Fixation buffer for 22h, resuspended according to 10x 16plex flex scRNAseq protocol in Quenching buffer+ Enhancer+ Glycerol and frozen at -80°C for at least 1-4 weeks. 10x Genomics Fixed RNA Profiling