llumina HiSeq 2500 of South American Histoplasma isolates
To prepare genomic libraries, we used KAPA library preparation kits for Illumina NGS sequencing (Kapa Biosystems) and 1g of purified DNA per isolate. Next, we indexed the libraries using unique 8-bp nucleotide identifiers. We evaluated the concentration of each library with a Kapa library quantification kit (Kapa Biosystems, Wilmington, MA) on a 7900HT Instrument (Life Technologies). We sequenced the libraries to a read length of 100 bp using v3 or v4 chemistries on an Illumina HiSeq 2500.