GSM8819803: Round spermatids, Tent5c wt/wt, rep3; Mus musculus; OTHER
Total RNA was isolated using TRIzol. Poly(A) RNAs were purified with oligoT beads. Direct RNA sequencing libraries were built according to the Oxford Nanopore Technologies protocol (Direct RNA Sequencing Kit- SQK-RNA002) with modifications to incorporate barcoded oligonucleotides. This analysis include 3 libraries per condition considered here as replicate (rep1-3). Each library is built with a pool of mRNAs isolated from the spermatids of 3 independant mice. Each library include four distinct barcodes to detect 3'end residues. Libraries were run on Nanopore R9 chips with a GridIon instrument.