4 h PAO1 inoculation after 24 h CL wear mouse corneal epithelium-2
RNA samples from mice that had not been inoculated with bacteria were processed with Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat). RNA from mice that had been inoculated with bacteria were processed with both the Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat) and Ribo-Zero rRNA Removal Kit (Bacteria) (Illumina, San Diego, CA). A Kapa Biosystems library preparation kit was used to prepare a standard-sized library with custom Unique Dual indexes.