GSM8889032: Control_Jejunum; Sus scrofa; RNA-Seq
Total RNA was extracted from pooled jejunum tissues using the TRIzol reagent (Invitrogen, USA) following the manufacturer's protocol. Single-cell RNA sequencing libraries were prepared using the 10x Genomics Chromium Single Cell 3' v3.1 Kit according to the manufacturer's protocol. Briefly, high-quality total RNA was converted to cDNA, which was then fragmented and amplified. Gel beads containing barcoded oligonucleotides were used to capture and label single cells. After reverse transcription, barcoded cDNA was amplified by PCR, followed by library construction. The constructed libraries were assessed for quality using an Agilent 2100 Bioanalyzer and quantified by Qubit Fluorometer (Thermo Fisher Scientific). Libraries were sequenced on an Illumina NovaSeq 6000 platform to generate 150 bp paired-end reads.