Flex RNA-seq of COLO320DM and COLO320HSR: cell culture
Fixed COLO320DM and COLO320HSR cells were processed using the 10x Genomics Chromium Fixed RNA Profiling workflow with custom probe hybridization. Libraries were generated targeting 60,000 cells, quality-checked, and sequenced on Illumina NovaSeq X with paired-end configuration (28-10-10-90 bp).