miRNA seq of IBD patients
Small RNA libraries were prepared using a NEBNext Small RNA Library Prep Set kit for Illumina according to the manufacturer's instruction. Each sample was pooled into a single library in equal quantities. Libraries were adjusted to a concentration of 4 nM using 10 mM Tris (pH 8.5). Library pool was denatured with 0.2 N NaOH. A standard 1% PhiX Control Library was also denatured and used as an internal control. Sequencing was carried out on Illumina NextSeq 550 Sequencing System with read lengths of 75 base pairs and 3.5 million single-end reads per sample, on average.