RNA-Seq of homo sapiens: A375 melanoma cells
To explore the function of LOT1,  A375 reporter or wild-type cells were transduced with lentivirus expressing CRISPR/Cas9 and indicated sgRNA, with irrelevant gRNAs targeting LacZ or Luciferase as negative controls. Seven days after selection with 10 g/mL puromycin, RNA was isolated and its integrity is evaluated using Agilent 4200 TapeStation. 1 g RNA per sample was used as input for the library preparation following standard protocols. Library preparations were sequenced on a NovaSeq 6000 platform (Chi-Biotech, Guangzhou, China) and 150 bp stand-specific paired-end reads were generated.