GSM9131384: KO1; Mus musculus; RNA-Seq
Tumor tissue was treated by BD Horizon Dri Tissue & Tumor Dissociation Reagent (BD Biosciences) and CD45.2+CD8+ cells were sorted (BD Symphony S6). Purified mRNAs were used for the library construction with the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB) according to the manufacturer's protocol.