Genomic sequencing of Fusarium graminearum
F. graminearum were collected from wheat-producing farms across Manitoba and grown on 25% PDA with 0.02% Streptomycin sulfate for 7 days under fluorescent light. DNA was extracted in TES extraction buffer and subsequently cleaned with the DNeasy PowerClean Pro Cleanup Kit. DNA libraries were prepared using the shotgun DNA library preparation (NEB Ultra II) and sequenced on the Illumina Shotgun HiSeq 2500 with 125-paired-end reads.