GSM9229778: atac-seq, OCI-AML3 cells, siNPM1c, Day3, rep2; Homo sapiens; ATAC-seq
Nuclei from 3 × 10^5 cells were isolated using a lysis buffer composed of 10 mM Tris-HCl, 10 mM NaCl, 3 mM MgCl2, 0.1% NP40, 0.1% Tween-20 and 0.01% Digitonin. Immediately after cell lysis, nuclei were pelleted in low-bind 1.5-ml tubes (Eppendorf) and resuspended in transpose mix (Vazyme) Transposition was performed at 37 °C for 30 min. After purification of the DNA with the VAHTS DNA Clean Beads (Vazyme) at a 2 × ratio, libraries were barcoded using TruePrep™ Index Kit V2 for lllumina (Vazyme TD202) and amplified for 5 cycles. Additional PCR cycles were evaluated by real time PCR. Final product was cleaned and size selected by VAHTS DNA Clean Beads at a 0.6 × / 0.7 × ratio.