GSM8450576: OV-FC + ACT treated [scRNA-seq]; Mus musculus; RNA-Seq
Tumor-draining lymph nodes (tdLN) were harvested, homogenized, and obtained cells were resuspended in 0.04% BSA in phosphate-buffered saline without calcium and magnesium, passed through a 70 μm cell strainer to receive single-cell suspension and washed three times in pre-chilled 1% BSA in PBS. Single-cell gene expression libraries were created using the 10x Genomics Chromium Next GEM Single Cell 3' Kit v.3.1 (10x Genomics) as per manufacturer recommendations.