DNA-seq of Guzmania for phylogenetic analysis
DNA-Seq libraries for Illumina NovaSeq sequencing were constructed using the NEBNext UltraTMII DNA Library Prep Kit for Illumina. DNA samples were fragmented and end repaired according to the user guide followed by adaptor ligation. T adaptor-ligated DNA were purified by Agencourt AMPure beads. The PCR was done for only 8 cycles of amplification in order to minimize duplicate reads. Barcoding was done using the indexing reagents provided in the NEBNext Unique Dual Index Oligos kit. The final libraries were purified by Agencourt AMPure beads.