RNA-seq of AMO-1 multiple myeloma cell line silenced for lncRNA NEAT1 expression and relative control condition
RNA-seq of AMO-1 multiple myeloma cell line silenced for lncRNA NEAT1 expression and relative control condition
Cells were collected after 96 hours from gymnotic delivery. AMO-1 were cultured in RPMI supplemented with 10% FBS and 1% Penicillin-Streptomycin. Cells were seeded at 5x10^4 cells/ml and concurrently treated with naked gapmeR (g#N1_E and g#SCR). Total RNA was extracted by TRIzol reagent according to the manufacturer's instructions. RNAseq libraries were prepared by Illumina® Stranded Total RNA Prep kit starting from 500 ng of RNA. RNA quality was assessed by 4200 Tapestation using RNA screen tape kit protocol.