GSM9777731: GSK3B wild-type RKO ATAC-Seq 1; Homo sapiens; ATAC-seq
ATAC sequencing service was provided by CloudSeq Biotech (Shanghai, China) with GenSeq® ATAC kit (GenSeq Inc.). Briefly, cells were lysed using cold lysis buffer. After lysis, nuclei were collected by centrifuging at 500 g for 10 min at 4℃. The pellet was transposed with transposome and tags were added on both sides of fragmented genomic DNA according to the manufacturer's instruction. After tagmentation, the purified tagged DNA was used for PCR amplification with GenSeq® 2×HiFi PCR Mix (GenSeq Inc.). The libraries were quantified using Qubit fluorometric assay (ThermoFisher).