sequences of bacteria; mus musculus;8 weeks; colonic contents; RT
PCR amplication of the bacterial 16S rRNA genes V3V4 region was performed using the forward primer 338F (5'-ACTCCTACGGGAGGCAGCA-3') and the reverse primer 806R (5'-GGACTACHVGGGTWTCTAAT-3'). Sample-specific 7-bp barcodes were incorporated into the primers for multiplex sequencing