GSM9251280: Splenic cDC1, Uqcfqf/f AOX-Rosa26, rep 3; Mus musculus; Bisulfite-Seq
Genomic DNA was harvested using the DNA Micro kit (Qiagen). Subsequent analysis was carried out by Macrogen NGS Service. Briefly, genomic DNA is bisulfite-treated, then sequentially adapter-tailed, extended, ligated, and amplified by indexing PCR to generate full-length libraries, with bead-based clean-ups at each step to remove small fragments and adjust buffer conditions.