GSM9229729: cGVHD sample 2 Flex singleplex; Mus musculus; RNA-Seq
Whole lungs were placed in dissociation media (Blank DMEM supplemented with 10mg/mL DNASE and 1.5mg/mL Collagenase IV) and dissociated via m_Lung_02 program of octoMACS dissociator, followed by an incubation at 370C/30min and a repeated dissociation. Solution was then filtered through a 100mM filter into a single cell suspension. Single cell suspensions were fixed and processed using the 10X Genomics NextGem Fixed RNA Profiling kit according to the manufacturer recommendations. We targeted 10,000 single cells per sample and multiplexed four samples together.