WES of tumour: adult male thumb
The genomic DNA was randomly fragmented into 180280 bp segments using a Covaris instrument. Library construction and capture experiments were done using the Agilent SureSelect Human All Exon V5/V6 kit. After end repair, phosphorylation, and A-tailing, adapters were ligated to both ends of the fragments to prepare the DNA library. The library with specific indexes was hybridized in solution with up to 543,872 biotin-labeled probes, and then 334,378 exons from 20,965 genes were captured using streptavidin-coated magnetic beads. After linear PCR amplification and library quality check, the qualified library was ready for sequencing.