GSM9157842: zs11_young_leaves_BS-seq; Brassica napus; Bisulfite-Seq
Total RNA from young leaves was isolated using the RNeasy Plant Mini Kit (QIAGEN, 74904). Approximately 2 μg of RNA was used for library preparation with the Illumina TruSeq RNA kit following the manufacturer's protocol; Libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit for Illumina and sequenced on an Illumina HiSeq X-Ten system (paired-end 150-bp reads). The libraries were sequenced on an Illumina HiSeq X-Ten system.