GSM9812785: hiSCs, lenti-control, rep2; Homo sapiens; RNA-Seq
Total cellular RNA was extracted using Trizol reagent. Messenger RNA (mRNA) was specifically enriched from the total RNA using oligo(dT) capture beads. RNA-seq libraries were constructed using the RNA-seq Library Prep Kit for Illumina (Cat. No. NR605, Vazyme, China), following the kit's recommended procedures, including RNA fragmentation, first-strand cDNA synthesis, second-strand cDNA synthesis, end repair, adapter ligation, and PCR amplification.