GSM9701346: B-LCLs, LPS-treated B-LCLs,high; Mus musculus; RNA-Seq
RNA was harvested using  Trizol Kit (BeyoPure). 1.3 ug of total RNA was used for the construction of sequencing libraries. Total RNA was extracted from [tissue/cell line] samples using the BeyoPure™ Total RNA Extraction and Purification Kit (Beyotime Biotechnology, China, Cat# RN0516) according to the manufacturer's instructions. RNA quality was assessed using a NanoDrop spectrophotometer (Thermo Fisher Scientific) and an Agilent 2100 Bioanalyzer (Agilent Technologies), with all samples exhibiting RNA Integrity Number (RIN) > 8.0. Poly(A)+ mRNA was enriched from total RNA using oligo(dT) magnetic beads. The RNA was then fragmented into short fragments (approximately 200–300 bp) under elevated temperature in fragmentation buffer. First-strand cDNA was synthesized using reverse transcriptase and random hexamer primers, followed by second-strand cDNA synthesis using DNA polymerase I and RNase H. The double-stranded cDNA libraries were constructed by end repair, 3'-end adenylation, adapter ligation, and size selection using AMPure XP beads (Beckman Coulter). Libraries were amplified by PCR and purified again. Finally, library quality was validated on the Bioanalyzer, and quantification was performed using qPCR. Libraries were sequenced on an Illumina NovaSeq 6000 platform with paired-end 150 bp (PE150) reads