genome re-sequencing of yeast:KO-2
Genomic DNA was extracted from sample cells and fragmented by sonication. After end repair, A-tailing and adapter ligation were performed, followed by size selection to retain target DNA fragments. PCR amplification and purification were conducted to complete library construction. Library concentration was quantified with Qubit and real-time PCR, and fragment size distribution was examined using a bioanalyzer prior to sequencing.