mRNA sequencing of Ca61R Xanthomonas euvesicatoria resistant pepper line carrying bs6 recessive resistance
The library for Illumina sequencing was prepared using NEBNext Ultra IIDirectional RNA Library Prep Kit for Illumina (NEB, Ipswitch, MA, USA).Briefly, the mRNA enrichment was carried out from 1 ug total RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, Ipswitch, MA, USA). Thereafter, the polyA enriched RNA wasfragmented, end prepped and adapter-ligated. Finally, the librarywas amplifiedaccording to the manufacturers instructions. The quality of the libraries waschecked on Agilent TapeSation System (AgilentTechnologies, Palo Alto, CA, USA), the quantity was measured on Qubit Fluorometer.Illumina sequencing was performed on the NextSeq 1000 instrument (Illumina, SanDiego, CA, USA) with 2  151run configuration.