GSM8626358: Biol rep 4, infected + GSK2606414; Mus musculus; RNA-Seq
Following infection, cells were lysed in triZOL. Samples were then mixed 5:1 with chloroform and shaken. Samples were then spun to separate layers and aqueous layer containing RNA collected. RNA isolation/purification was then performed using Direct-zol RNA MiniPrep kit. Libraries were prepared by Azenta using the NEBNext Ultra II RNA Library Prep Kit with polyA selection per manufacturer's instructions