PacBio HiFi reads for Strix uralensis 1
DNA was extracted from a skin biopsy using the Monarch HMW DNA Extraction Kit (NEB, Ipswich, USA). High-molecular weight status was validated by quality control with capillary electrophoresis (Agilent Femto Pulse) and a SPK 3.0 PacBio HiFi library was prepared according to the recommendations by the vendor. Next, HiFi SMRT sequencing was performed on two SMRT cells (8 mio ZMWs) on a PacBio Sequel IIe (Pacific Biosciences, Menlo Park, USA).