GSM9670805: biological replicate-2 Cyc8-non-Anchor-away Snf2-HA (IP); Saccharomyces cerevisiae; ChIP-Seq
Following ChIP protocol (used anti-HA antibody from Abcam (ab9110)), input and IP chromatin samples had cross-links reversed at 65oC with high salt in the presence of Pronase to digest protein. Input and IP DNA was finally purified through a Quiagen QIAquick PCR purification kit. The extracted DNA was sent to Azenta life sciences for library preparation and pair end sequecing using Illumina NovaSeq 6000 platform