GSM9372008: PCLS-EV, Aged Uninjured, Rep 2; Mus musculus; miRNA-Seq
PCLS-EVs were isolated using size exclusion chromatography (IZON, ICO-35) from media used to culture 30 PCLS for each physiological condition. Total RNA was extracted from EVs with Direct-zolTM RNA Microprep kit (Zymo Research, R2060). RNA was prepared for small RNA sequencing using the QIAseq miRNA Library Kit (Qiagen). Quality control of the libraries was performed using the Bioanalyzer 2100 High Sensitivity DNA Assay (Agilent) and quantified using the Qubit dsDNA HS (High Sensitivity) Assay Kit (Thermo Fisher)