GSM9202978: Ma-Mel-94 cells, siNC and 17-DMAG, rep3; Homo sapiens; RNA-Seq
Total RNA was extracted using the Maxwell RSC simplyRNA Cells Kit (Promega) following the manufacturer's instructions. RNA concentration was measured using the QuantiFluor RNA System (Promega). 5 µg of total RNA was used for library construction. For library preparation, 5 µg of total RNA was used as input for poly(A) RNA enrichment using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Libraries were then prepared using the NEBNext Ultra II Directional RNA Library Prep Kit Illumina (New England Biolabs), with all samples processed in a single batch. The resulting mRNA libraries were assessed using the High Sensitivity D1000 DNA ScreenTape (Agilent Technologies) and quantified using the QuantiFluor dsDNA System (Promega).