SHAPE-MaP_tRNA_Pro_Dic_5NIA_1
tRNA sequences were in-vitro transcribed. RNA structure was then probed using Selective 2_-Hydroxyl Acylation Analyzed by Primer Extension (SHAPE). We probed the tRNAs with either dimethyl sulfate (DMS), a potent methylation agent for adenine and cytosine that produces N_-methyladenine and N_-methylcytosine in exposed nucleobases, or the SHAPE reagent 5-Nitroisatoic Anhydride (5NIA), which reacts to form a chemical adduct (2-O) at the 2_ hydroxyl group of an accessible ribose via ester linkage. These mutations can then be profiled through sequencing and structure analysed. Sequencing library prep kit used was xGen RNA Library Prep Kit (IDT), using Illumina multiplex oligos.