16S rRNA gene amplicon sequencing of sediment microbial communities, UC30 wreck
Genomic DNA was submitted to LGC Genomics (Berlin, Germany) for library preparation and Illumina MiSeq sequencing (v3 chemistry, 2300 bp paired-end) using primers 341F (5'-CCTACGGGNGGCWGCAG-3') and 785Rmod (5'-GACTACHVGGGTATCTAAKCC-3'), targeting the V3V4 hypervariable regions of the 16S rRNA gene.