RNA-Seq of Homo sapiens: U937 macrophages, USP30-AS1 knockout
Total RNA was extracted from USP30-AS1 knockout U937 macrophage cells using the TRIzol reagent (Thermo Fisher). RNA quality was assessed using a Bioanalyzer (Agilent) to ensure integrity. RNA-seq libraries were constructed using the NEBNext Ultra II RNA Library Prep Kit (New England Biolabs), following the manufacturers protocol. Libraries were sequenced on an Illumina NovaSeq 6000 platform with paired-end reads (150 bp).