GSM9813557: HCT116 H4R3me2a ChIP-seq; Homo sapiens; ChIP-Seq
CRC cells (~10^7 per sample) were cross-linked with 1% formaldehyde for 10 min, quenched with glycine and lysed. Chromatin was sheared to 200-500 bp by sonication. Immunoprecipitation was performed overnight at 4 C with H4R3me2a antibody or input DNA control, captured with Protein A/G magnetic beads. After washing, cross-link reversal and DNA purification, DNA was used for library preparation. Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit and sequenced as paired-end 150 bp reads on an Illumina NovaSeq 6000.