GSM9072962: M2; Mus musculus; RNA-Seq
The scRNA-seq data included 59,197 cells from 3 Control samples, 3 De_Chlamydia-treated samples and 3 Chlamydia-treated mouse large intestine tissue samples. Mice aged 6 to 7 weeks received intragastric inoculations of purified C. muridarum EBs (either live  or heat deactivated), with each mouse receiving 2 × 10^5 inclusion-forming units (IFUs). The naïve control group was administered a sucrose-phosphate-glutamic acid (SPG) vehicle. Single-cell cDNA libraries were generated as described in Single cell 3' whole transcriptome amplification (WTA) protocol (BD Rhapsody,USA) using successfully prepared single-cell suspensions. Single cells washed three times, pooled, counted and subsequently loaded onto a cartridge, lysed inside the wells followed by mRNA capture on cell capture beads according to manufacturer instructions. Cell Capture Beads were retrieved and washed prior to performing reverse transcription and treatment with Exonuclease I. cDNA underwent extension using the WTA extension primers, and purified using SPRIselect magnetic beads (B23318, Beckman Coulter,USA).The quantity of PCR products was determined by Qubit 4.0 with Qubit dsDNA HS Assay (Q32854, Thermo Fisher Scientific,USA). For high-throughput sequencing, the libraries were prepared following the manufacturer's instructions and applied to Illumina Novaseq 6000 system for 150 nt paired-end sequencing.