RNA-seq of Medicago truncatula nsp2_control_3
For RNA-seq of nsp2-2 mutant after rhizobial inoculation, the whole roots from FP agar plates were collected at 7dpi. Total RNA was isolated with the RNeasy Plant Mini Kit (74904, Qiagen), following the manufacturers instructions. The sequencing of the libraries was performed by Novogene Europe (Cambridge, UK) with the read length of 150 bp paired-end reads.