Metabarcoding of Triatoma costalimai, specimen 10
DNA was extracted from the whole gut of individual Triatoma costalimai nymphs collected from peridomestic environments in the Brazilian Cerrado. Amplicon libraries targeting the bacterial 16S rRNA V3-V4 region, fungal ITS1 region, vertebrate 12S rRNA gene, and Trypanosoma cruzi satellite DNA were generated by PCR, pooled, indexed with Nextera XT adapters, and sequenced on an Illumina MiSeq platform using paired-end 301 bp reads.