16S rRNA amplicon sequencing of Macrobrachium rosenbergii: water microbiota
Total genomic DNA was extracted from intestinal contents using the E.Z.N.A. Soil DNA Kit (Omega, USA). The V3V4 hypervariable region of the bacterial 16S rRNA gene was amplified using specific primers. PCR products were purified, and sequencing libraries were constructed using the TrueSeq DNA Sample Prep Kit. Paired-end sequencing (2  300 bp) was performed on the Illumina NextSeq 2000 platform.