GSM9814090: Control 2; Homo sapiens; RNA-Seq
Total RNA of cells or tissues were isolated with RNA extraction reagent (Servicebio, Cat#: G3013). cDNA was synthesized from 1 μg of total RNA with SweScript RT II First Strand cDNA Synthesis Kit (Servicebio, Cat#: G3333.). 1.3 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kitollowing manufacturer's protocols.