early post-transplant stool sample from Liver Transplant patient UC-066
Fecal samples should be aliquoted within 24 hours into 1.5 ml freezer vials and stored at -80C. DNA extraction is performed using the QIAamp PowerFecal Pro DNA Kit and libraries were prepared using 100-200 ng of genomic DNA using the QIAseq FX DNA library kit (Qiagen).  Briefly, DNA was fragmented enzymatically into smaller fragments and desired insert size was achieved by adjusting fragmentation conditions. Fragmented DNA was end repaired and As were added to the 3ends to stage inserts for ligation. During ligation step, Illumina compatible Unique Dual Index (UDI) adapters were added to the inserts and the prepared library was amplified. Libraries were then cleaned up, and library size was measured using a TapeStation (Agilent). Sequencing runs are performed on the Illumina NextSeq platform in the Functional Genomics Facility at UChicago using the 2X150 Paired End reads cassette.