GSM8712396: Control, replicate 3; Homo sapiens; RNA-Seq
Total RNAs were extracted and purified with the RNeasy Plus Micro Kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol. NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific) and 2200 Tape Station Automated Electrophoresis System (Agilent Technologies, Santa Clara, Calif) were used to determine the quantity and quality of the isolated RNA. The samples with RNA integrity numbers 9.4 or more were chosen for sequencing. RNA sequencing (RNAseq) was performed on Illumina NovaSeq 6000 with the TruSeq RNA Stranded Sample Prep Kit (Illumina, San Diego, Calif).