GSM9777775: H1FX/S31A(repeat 1); Homo sapiens; ATAC-seq
50000 cells were treated with cell lysis buffer then centrifuging for 10 min at 500g, supernatant (cytoplasm) was discarded and pellet (nuclei) was keeped. Transposition mix (Vazyme,TD711) was added to nuclei and incubated at 37℃ for 30 minutes on thermomixer at 1,000 rpm. Tagmented DNA was recovered using ATAC DNA Extract Beads (Vazyme,TD711) then amplified by PCR using indexed P5 and P7 primers. The library products were enriched, quantified and finally sequenced on a DNBSEQ-T7 (MGI) platform using 150 bp paired end reads.