RNA-Seq of Babesia bigemina
The construction of the RNA libraries was carried out following the protocol of the TruSeq Stranded mRNA Sample Preparation Kit from Illumina. Once constructed, the libraries were analyzed to determine the average library size through a capillary electrophoresis assay using a Bioanalyzer 2100 from Agilent with a DNA 1000 chip, and the concentration was determined using a fluorometric method with the Qubit 1X dsDNA HS Assay Kit and a Qubit 4.0 from Invitrogen.