Whole Genome Sequencing of Wild Schizophyllum
High-quality genomic DNA was extracted from fungal mycelium and used for whole-genome library preparation. DNA was fragmented to the desired insert size, followed by end repair, A-tailing, adapter ligation, and PCR enrichment according to the manufacturer's instructions. Library quality was assessed using fluorometric quantification and fragment analysis. Sequencing was performed on an Illumina platform to generate paired-end reads for whole-genome analysis.