Lactuca saligna CGN05327 ONT UL exp1
High-molecular-weight genomic DNA was isolated from 6g of young seedlings using the Nanobind Plant Nuclei Big DNA Kit. Ultra-long (UL) nanopore sequencing libraries were prepared using Ultra-Long DNA Sequencing Kit V14 (SQK-ULK114, Oxford Nanopore Technologies) following manufacturers guidelines. UL libraries were sequenced using R10.4.1 flow cell on a PromethION P24 device. Sequencing run was set up using MinKNOW - PromethION 24.06.10. Data was basecalled live during the run with ont-dorado-server-for-promethion 7.4.12.