1640 medium without serine or glycine of P17
The AML cell line Molm13 stably expressing Cas9 (namely Molm13-Cas9) was constructed and infected with a pool of lentiviruses targeting 423 epigenetic genes as well as positive and negative controls at a multiplicity of infection (MOI) < 0.3 (Fig 1C). After puromycin selection, the pool of KO cells was maintained in complete medium (CM) or medium deprived of SG (-SG) and collected after 0, 8, 12, and 17 population doublings for massively parallel sequencing of all sgRNAs.