GSM9290758: NaïveCD8s; Homo sapiens; ATAC-seq
Fragmented DNA were purified by Qiagen MinElute Enzyme Reaction Cleanup Kit (Qiagen, 28204) per the manufacture's protocol. ATAC-seq libraries were prepared by PCR amplification of DNA fragments using Nextera Index Kit Transposed DNA was prepared with Tn5 (Illumina #FC-121-1031) as described in Buenrostro et al. 2013 using 0.1% Tween 20 instead of IGEPAL CA-630.Libraries were barcoded with Nextera Index Kit (Illumina # FC-121-1012) ,Libraries were quantified using the KAPA Library Quantification Kit (Roche, KK4824) and sequenced on a NextSeq 550 platform