Whole Genome Sequencing of Novel Bacteriophages Targeting Methicillin-Resistant Staphylococcus aureus
High quality genomic DNA (gDNA) was subjected to purity check spectrophotometrically to measure the A260/280 ratio and its concentration was measured using Qubit broad range dsDNA quantification kit (Thermo Fisher Scientific). 100ng of total DNA was used as input for library preparation using HiMedia library preparation kit(MBNGS001) to fragment and obtain adapter ligated and indexed library as per manufacturer's instructions. The indexed library was sequenced on an Illumina NextSeq 2000 using a 300 cycle paired end chemistry.