GSM8539302: 1-3; Mus musculus; RNA-Seq
Total RNA of whole brain (Exclusion of cerebellum and olfactory bulb) was isolated with elimination of DNA contamination using DNase. mRNA was first purified from the total RNA using poly-T oligo-attached magnetic beads and then fragmented, after which cDNA synthesis was performed. Then the synthesized cDNA was used for the preparation of PE100 strand specific sequencing libraries. Strand-specific RNA-seq