GSM8712443: N2AIP_F1; Mus musculus; ChIP-Seq
Each immunoprecipitation reaction was conducted in ChIP dilution buffer for 3 h at 4 °C using 150 µg of chromatin and 40 µl FLAG Affinity Gels (catalogue no. F2426; Merck Millipore). After the gels were washed as above, the crosslinked DNA-protein complexes were eluted by incubation with 90 µg of 3XFlag peptide (catalogue no.P9801; Beyotime) at 4 °C for 1 h. DNA libraries were constructed and sequenced by Tianjin Novogene Bioin_x0002_formatic Technology Co., Ltd. Briefly, an NEBNext® Ultra™ II DNA Library Prep kit (catalogue no. E7645L; NEB) was used for library preparation.