GSM8594835: KN1 protoplast input; Zea mays; ChIP-Seq
The isolated protoplasts were transfected by p1107-KN1 plasmid. We performed BLRP-ChIP-Seq on the cells. DNA was purified using the ChIP DNA Clean & Concentrator™ kit (Zymo D5205). DNA concentration was measured using Qubit. The cells were incubated in dark overnight. We used NEBNext® Ultra™ II DNA Library Prep Kit (NEB E7645S) for sequencing libraries. The libraries were sequenced on the Illumina NextSeq 500 platform at NYU-CGSB Genomics Core using the HighOutput 1x75bp mode.