GSM8890082: mouse KC pancreas, DIO, 3; Mus musculus; RNA-Seq
Total RNA was extracted using the RNeasy mini kit (QIAGEN), and residual amounts of DNA were removed using an on-column RNase-Free DNase Set (QIAGEN) Library preparation and RNA-sequencing were performed by Novogene Co. mRNA was enriched using oligo(dT) beads, and rRNA was removed using the Ribo-Zero kit. The mRNA was fragmented, and cDNA was synthesized by using mRNA template and random hexamers primer, after which a second-strand synthesis buffer (Illumina), dNTPs, RNase H and DNA polymerase I were added for the second-strand synthesis, followed by adaptor ligation and size selection.