16S rRNA sequencing of activated sludge from a natural pigment factory
The V3-V4 region of the 16S rRNA gene was amplified using primers with overhang linker sequences 341F (5-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG~1~7N~CCTACGGGNGGCWGCAG-3) and 805R (5-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG1~7N~GACTACHVGGGTATCTAATCC-3). Amplicons generated through the steps described above were purified using the AMPure XP system (Beckman Coulter). Library preparation was performed using the Nextera XT Index Kit v2 Set (Illumina, San Diego, CA, USA). Qualified libraries were then sequenced on the Illumina MiSeq platform using a 300 bp paired-end read configuration.