GSM8818843: Dam-control, batch1, NSCs; Drosophila melanogaster; OTHER
Genomic DNA was extracted and digested by DpnI (targets only methylated GATC sites). After that, methylated DNA was amplified by PCR The Illumina TruSeq DNA PCR-free library preparation kit were used according to the manufacturer's protocol. Briefly, DNA was fragmented and end-repaired first. Then an adenine (A) nucleotide was added to the 3ʹ ends of the fragments to prevent self-ligation. After that, index adaptors were ligated and followed by quantifying. normalizing and pooling of libraries. The libraries were sequenced on the illumina MiSeq equipment. Libraries were constructed from the amplified DNA samples using the illumina TruSeq DNA PCR-free library preparation kit according to the manufacturer's protocol and sequenced on the illumina MiSeq equipment. Paired reads (2 × 250 bp) were sequenced from Dam-Simba1/2 or Dam-Control library.