16S rRNA sequencing of phycosphere microbiota: V3-V4 region amplicons from in vitro algal-bacterial co-cultur
The V3-V4 hypervariable region of the 16S rRNA gene was amplified from genomic DNA using the 341F/805R primer pair with Illumina adapter sequences. The resulting amplicons were purified. Library concentration was quantified using the Qubit 4.0 dsDNA HS Assay, and fragment size distribution was validated on an Agilent 2100 Bioanalyzer. The library was sequenced on an Illumina MiSeq platform with a paired-end (2x300) strategy.