First report of blaCTX-M-15 in a marine Enterobacter isolate from Bangladesh: genomic insights into halotolerance and pathogenicity
Genomic DNA of isolate LB01 was extracted from overnight cultures grown in Nutrient Broth using the TIANamp Genomic DNA Kit (Tiangen Biotech, China) according to the manufacturers instructions for bacterial cells. DNA quality and concentration were evaluated using a NanoDrop spectrophotometer (Thermo Fisher Scientific, USA). Sequencing libraries were constructed using the Nextera XT DNA Library Preparation Kit (Illumina, USA) and sequenced on an Illumina platform to generate 2  150 bp paired-end reads.