GSM9558285: RNA, HEK293T, pLEX, dmso,12h, rep3; Homo sapiens; RNA-Seq
Total RNA was extracted with RNAseasy lipid tissue kit. mRNA was enriched with oligo dT beads that target the poly A tail. mRNA molecules were fragmented and synthesized into first strand cDNA using random primers. The second strand was synthesized with dUTP instead of dTTP and cDNA was subject to end-repair and 3' adenylation. Adaptors were then ligated to the 3' adenylated cDNA fragments after which were PCR amplified. Libraries were then circularized and amplified to make a DNA nanoball and sequenced on the DNBSEQ-G400.