GSM9557743: MLE-12 cells,Hyperoxia3,24h; Mus musculus; RNA-Seq
Total RNA was extracted from samples using RNeasy Mini Kit (Qiagen, Germany) according to the manufacturer's instructions. RNA quantity and quality were assessed prior to library construction. RNA libraries were prepared using the TruSeq® RNA Sample Preparation Kit (Illumina, USA) following the manufacturer's instructions. Briefly, poly(A)+ mRNA was enriched using oligo(dT) magnetic beads, followed by fragmentation and cDNA synthesis. Libraries were quantified using Qubit 2.0 Fluorometer and validated using Agilent 2100 Bioanalyzer. Sequencing was performed on the Illumina NovaSeq 6000 platform.