Short read Pacbio ONSO sequences of  Diaphorobacter sp. DS2
Library preparation was performed using the QIASeq FX DNA library preparation protocol for the ONSO platform (Cat#180475) by following the manufacturers instructions. About 50 ng of Qubit quantified DNA was enzymatically fragmented, end-repaired and A-tailed in a one-tube reaction using the FX Enzyme Mix provided in the QIASeq FX DNA kit. The end-repaired and adenylated fragments were subjected to adapter ligation, whereby an index-incorporated Illumina adapter was ligated to generate a sequencing library. The library was subjected to 6 cycles of Indexing-PCR (Initial Denaturation at 98C for 2 min, cycling (at 98C for 20sec, 60C for 30sec, 72C for 30sec) and final extension at 72C for 1min) to enrich the adapter-tagged fragments. Finally, the amplified library was purified using Cambrian Bioworks CamSelect NGS(#CBWD010), followed by a library quality control check. Illumina-compatible sequencing libraries were quantified by Qubit fluorometer (Thermo Fisher Scientific MA, USA), and fragment size distribution was analysed using Agilent 4150 TapeStation.