GSM9703183: Triple transfection replicate 2; Homo sapiens; RNA-Seq
Total RNA was extracted using RNeasy Mini Kit (Qiagen, Cat# 74104) according to the manufacturer's instructions. RNA concentration was measured using Qubit fluorometer (Invitrogen). RNA quality was assessed using Agilent High-Sensitivity RNA ScreenTape, and samples with RNA integrity number (RIN) >7 were used for library preparation. mRNA-seq libraries were prepared using a modified NEBNext Ultra II Directional RNA Library Prep Kit protocol.