GSM8819250: EpiDermFT, ZDL, rep 2; Homo sapiens; RNA-Seq
After the treatment period, tissues were rinsed and RNA extraction was performed using the commercial RNeasy Plus Mini kit (Qiagen, Germantown, MD; cat. no. 74134). The QiaCube Connect robotic station was utilized to ensure a standardized extraction process. RNA libraries for RNA-seq were generated following a standard workflow with mRNA enrichment, mRNA fragmentation and random priming following by first and second strand cDNA synthesis with end repair, 5' phosphorylation and dA-tailing. Sequencing was subsequently carried out following adaptor ligation and PCR enrichment.