GSM9557201: P. maniculatus nubiterrae replicate 1; Peromyscus maniculatus nubiterrae; RNA-Seq
The anterior half of the cortex was extracted from both hemispheres for each animal (this includes all of sensorimotor cortex, as well as other areas) and flash-frozen in liquid nitrogen. Samples were stored at -70°C until further processing. Nuclei were extracted as described in Matson et al. (PMID 30371670) except that the high sucrose buffer had 1.2M sucrose. Nuclei were fixed using the Parse Evercode Fixation kit, according to the instructions of the manufacturer. Fixed nuclei were stored at -80C for up to 3 months. Library construction was performed using the Parse Evercode WT Mega kit according to the instructions of the manufacturer. Samples from other projects were included in the run. The data here is de-multiplexed by sample, so as to include only the data for this project.