16S rRNA gene V3-V4 sequencing of DNA isolated from mouse skin swabs using a Zymo Quick DNA Fecal/Soil Microbe Miniprep Kit or Quick-DNA/RNA Miniprep Plus Kit
Amplification of 16S rRNA gene V3-V4 region using primers fwd: 5' AGRGTTTGATCMTGGCTCAG 3' and rev: 5' GWATTACCGCGGCKGCTG 3'. Dual-indexing usng Illumina Nextera XT V2 primers and sequencing in 2x300bp mode on an Illumina MiSeq.