GSM8678182: RPE cells, 5-month-old mouse 2_left eye; Mus musculus; RNA-Seq
Murine RPE cells used for RNA-seq or cell culture were isolated following an established protocol (J. Liu et al., 2024; Scott et al., 2021).RNA of RPE cells was extracted using the RNeasy Mini kit (Qiagen). Library preparation was conducted using Illumina Stranded Total RNA Prep with Ribo Zero Plus (Illumina), followed by sequencing using the Illumina NextSeq 550 platform with 2×75 bp by paired-end reads at a depth of 16 million reads.