16S rRNA amplicon sequencing of Mus musculus: MODEL group feces (APP/PS1)
Fecal DNA extracted (PowerSoil). V4 amplification with 515F/806R primers. Indexed by Nextera XT. Purified (AMPure XP). Quantified (Qubit dsDNA HS). Sequenced on Illumina MiSeq 2x250bp.