Pseudomonas sp. WMBT8 genome from Sculpin GI tract
Genomic samples were fragmented using the Covaris g-TUBE following recommendedPacBio procedures. Sequencing libraries were prepared following the PacBio SMRTbellprep kit 3.0 with the SMRTbell barcoded adapter plate 3.0. Pooled libraries underwent thePacBio binding and cleanup steps following the Sequel II binding kit 3.2 and were loadedonto the PacBio Sequel IIe for a 15hr collection period with options enabled to retain subreadsand sequencing metrics. Demultiplexing, quality control, and adapter trimming was performedon instrument via Lima.