GSM8595713: SiLipids_2h_3; Hordeum vulgare; RNA-Seq
RNA extraction, cDNA synthesis and qRT-PCR were performed as described previously (Sarkar et al., 2019). Briefly, RNA was extracted using Trizol (Life Technologies, 15596018) and remaining DNA was removed by DNAseI (Thermo Scientific, EN0521) digestion at 37 °C for 30 min. For Illumina-compatible RNAseq libraries at first an enrichment for poly-A RNAs was performed (NEBNext® Poly(A) mRNA Magnetic Isolation Module; New England Biolabs), followed by library generation with NEBNext Ultra™II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Next, sequencing-by-synthesis was done on a NextSeq 2000 device in 2 x 150 bp paired-end read mode