GSM9293686: Lung,Pdcd1^high CD4 T cells,rep1; Mus musculus; RNA-Seq
Total RNA was extracted using the RNeasy Plus Micro Kit (Qiagen, Germany) following the manufacturer's instruction. RNA quality and quantity were assesed with the 4150 TapeStation Instrument (Agilent Technologies). cDNA libraries were generated from low-input total RNA using the NEBNext Low Input RNA Library Prep Kit for Illumina (#E6420S/L), according to the manufacturer's protocol, and sequencing was performed on an Illumina NovaSeq X Plus platform.