RNA-seq of HEK293 cells treated with 76nt dsRNA  (variant A)
Stranded RNA-seq of HEK293 cells following RNA transfection. Cells were transfected with RNA using Lipofectamine 2000 and incubated for 24 hours. Total RNA was extracted, ribosomal RNA was depleted using NEBNext rRNA Depletion Kit, and stranded libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit. Three biological replicates were sequenced using paired-end 150 bp reads to assess transcriptome-wide changes following RNA transfection.