RAW264.7 cells OE Control
CUT&Tag assay for H3K18la was performed using the Hyperactive In-Situ ChIP Library Prep Kit (Vazyme Biotech). Permeabilized cells were bound to ConA beads and incubated with primary antibody, secondary antibody, and pA-Tn5 transposase. Targeted tagmentation added Illumina adapters, followed by PCR amplification. Libraries were quality-controlled on an Agilent 2100 Bioanalyzer and sequenced on an Illumina NovaSeq 6000 with 150-bp paired-end reads.