GSM8819527: NbD413_505_mouse_1; Mus musculus; RNA-Seq
Single-cell suspensions were processed using the 10x Genomics Chromium Controller for single-cell partitioning and barcoding. The samples were processed using 10X chromium platform (Chromium Next GEM Immune Profiling 5'HT kit v2, 1000374). Cells were encapsulated into Gel Beads-in-Emulsion (GEMs) for droplet-based lysis and reverse transcription. Barcoded cDNA was then synthesized within the droplets, followed by amplification and purification according to the manufacturer's protocol (10x Genomics) Library construction was performed using the 10x Chromium Next GEM Immune Profiling 5' HT Kit v2 (Catalog #1000374) following the manufacturer's protocol (10x Genomics).