WGS of homo sapiens: male saliva
Genomic DNA (=750?ng) was sheared in a 96-well format using the Covaris LE220 focused ultrasonicator, targeting ~380?bp inserts. The sheared DNA was cleaned with Agencourt AMPure XP beads and then prepared using the KAPA Hyper Prep kit (KR0961 v1.14), which includes end-repair, A-tailing, and adapter ligation steps. Two additional AMPure cleanups removed excess adapter dimers. All library construction steps were automated on a Perkin Elmer Janus platform. Library yield and quality were assessed using the Invitrogen Quant-IT dsDNA High Sensitivity kit (Q33120), and libraries were validated with the Biorad CFX384 Real-Time System and KAPA Library Quantification Kit (KK4824). Final barcoded libraries were pooled robotically and sequenced on an Illumina NovaSeq 6000 instrument using paired-end sequencing.