Gut Microbiome of Active Spreading Vitiligo Patient 9
Genomic DNA from stool was purified using genomic DNA concentration kit (zymo research, USA) to remove inhibitors. The library was prepared using Nextra DNA Flex kit(illumina, USA). The simple process is as follows. i) Tagmention : using a transposase enzyme to simultaneously fragment and tag DNA in a single-tube reaction. ii) Amplification : Generation of NGS library structure through dual index primer amplification. iii) Beads clean up : 0.6X AMPure XP beads purified. The produced library was checked for library quality using Tapestation D1000 Tape and sequenced using Novaseq 6000(illumina, USA) equipment according to the Illumina sequencing guide.