GSM8712461: Untreated MC3T3-E1 (mouse pre-osteoblastic) cells cultured in the OC medium replicate 3; Mus musculus; RNA-Seq
RNA was extracted and purified using the miRNeasy Mini kit (Qiagen) following the manufacturer's protocol. Poly(A) RNA sequencing library was prepared following Illumina's TruSeq-stranded-mRNA sample preparation protocol. RNA integrity was checked with Agilent Technologies 2100 Bioanalyzer. Poly(A) tail-containing mRNAs were purified using oligo-(dT) magnetic beads with two rounds of purification. After purification, poly(A) RNA was fragmented using divalent cation buffer in elevated temperature.