RNA-Seq of human ovarian fibroblasts
Human ovarian fibroblasts culture in six-well plates were starved overnight and stimulated with IL-11 (10 ng/ml) for 24 h, total RNA was extracted using the phenol/chloroform method. The mRNA with polyA structure in total RNA is enriched using Oligo(dT) magnetic beads, and the RNA is fragmented into segments of approximately 300bp in length through ion disruption. cDNA sequencing libraries were prepared according to the manufacturers protocol and sequenced on an Illumina Novaseq 6000.