Raw sequencing data from the survey of Saussurea hypsipeta
This project begins with rigorous quality control of RNA samples: concentration is measured using Qubit 4.0 (total amount  1 g), integrity is assessed using Agilent 2100 (RIN value > 7, 28S/18S  2.0), and purity is verified using NanoDrop (A260/A280  2.0, A260/A230  2.0). Qualified samples are processed through the DNBSEQ platform for library construction. mRNA is captured using Oligo(dT) magnetic beads, fragmented, and synthesized into double-stranded cDNA. The library is constructed through end repair, A-tail addition, adapter ligation, and PCR amplification. After passing Qubit quantification and Agilent 2100 fragment distribution detection (peak ~350 bp), the library undergoes PE159 paired-end sequencing to generate raw FASTQ data.