GSM9101167: ARMC5 WT mouse kidney cortex, biological replication 1; Mus musculus; RNA-Seq
Tissue RNA was extracted using the RNeasy Mini Kit (Qiagen) following the manufacturer's protocol. The integrity and quantity of the isolated RNA were assessed using a 2100 Agilent Bioanalyzer (Agilent Technologies) with an RNA 6000 Pico kit Libraries were prepared from an equal quantity of total RNA for each sample (123 ng). First, ribosomal RNAs were depleted using a KAPA RiboErase (HMR) kit, and then libraries were prepared using a KAPA RNA Hyperprep Kit (Roche Diagnostics) with an 11-cycle final amplification