16S rRNA metabarcoding (V3-V4) of human gut microbiome:1729107F1719276
Metabarcoding analysis of the V3-V4 variable regions of the 16S rRNA gene to profile the human gut microbial community. The study follows a crossover design to evaluate the impact of BP 3:1 on the microbiome composition. Genomic DNA was extracted from fecal samples, and the V3-V4 library was prepared using the standard Illumina protocol. Sequencing was performed on the Illumina Illumina MiSeq i100 platform with a paired-end 2x300bp configuration.