Transcriptome profiling of HEK293F cells and RIP-seq analysis of ZATT-interacting transcripts
RNA-seq experiments were performed on three biological replicates per sample. Total RNA was extracted from HEK293F cells treated with DMSO 16 h, using RNeasy Mini Kit (Qiagen). The concentration of RNA was measured using Qubit, and the integrity was measured by TapeStation. All samples contained a RIN value of 10, and about 550 ng of purified total RNA was submitted to the NIEHS sequencing core for library preparation followed by sequencing.