WGS of cyoB deleted E. coli K12 MG1655
Genomic DNA was isolated using a Nucleospin Tissue kit (Macherey Nagel 740952.50) followingthe manufacturers protocol, including treatment with RNase A. Resequencing libraries were prepared using a Nextera XT kit (Illumina FC-1311024) following themanufacturers protocol. Nextera chemistry uses transposon-based DNA fragmentation whereby a transposon cleaves and tags DNA with adapters.