Mus musculus
TRIzol (Invitrogen, CA, USA)RNANanoDrop ND-1000 (NanoDrop, Wilmington, DE, USA)RNABioanalyzer 2100Agilent, CA, USARNAOD260/280>1.8total RNA>1goligo(dT)PolyAmRNAmRNARNAcDNARNase HcDNADNAAT300bp50bp(Hieff NGS Ultima Dual-mode mRNA Library Prep Kit for Illumina)illumina Novaseq 6000 PE163