GSM8713007: NC, C12; human feces metagenome; OTHER
Stool DNA extraction was performed according to the manufacturer's protocol using a QiAamp DNA Stool Mini Kit (Qiagen). The extracted DNA was stored in 1X ATE buffer (10 mM Tris-Cl pH 8.3, 0.1 mM EDTA, and 0.04% NaN3) at -20°C for subsequent 16S rRNA-targeted sequencing. PCR was performed using the forward primer 5′- TCG TCG GCA GCG TCA GAT GTG TAT AAG AGA CAG CCT ACG GGN GGC WGC AG-3′ and reverse primer 5′- GTC TCG TGG GCT CGG AGA TGT GTA TAA GAG ACA GGA CTA CHV GGG TAT CTA ATC C-3′ for the V3–V4 region of the 16S rRNA gene. Amplicons generated from each sample (~ 330 bp) were purified using AMPure XP magnetic beads (Beckman Coulter).