GSM8711690: The hippocampus  of C57BL/6 mice, 2 month with ID 1; Mus musculus; RNA-Seq
Perfusion with 0.1mol/L PB for cardiac perfusionis , wait for the liver to turn white, quickly obtain the cerebral cortex, hippocampus, kidneys tissue samples , stored with 1 ml of RNA later cryovials and transfer with dry ice. Using Agilent 2100 Bioanalyzer (Agilent RNA 6000 Nano Kit) to do the total RNA sample QC: RNA concentration, RIN value, 28S/18S and the fragment length distribution. For plant and fungi samples, we use NanoDropTM to identify the purity of the RNA samples. The library was constructed using classical OligodT magnetic beads to enrich mRNA.