GSM8752169: OE CUT&Tag; Homo sapiens; OTHER
0.1 million cells were collected and sequentially incubated sequentially with ConA beads, primary antibody (anti-H3K9me3 antibody, #A-4036, EpiGentek) and secondary antibody (Goat anti-Rabbit IgG, Vazyme) hyperactive PG-TN5/PA-TN5 Transposon was used for each CUT&Tag reaction, and CUT&Tag libraries were constructed using the Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro protocol (Vazyme, #TD904).