RNAseq of S. cerevisiae monoculture
RNA was extracted from the frozen cell pellets using a modified method from Collart and Oliviero (1993) and stored at -80 C. RNA QC using an Agilent TapeStation, library preparation, hybridisation and Library QC (PacBio, California, United States; Cat no. 102-431-400; 102-431-700; 102-410-800; 102-427-200) and final sequencing was performed by Inqaba Biotech (Pretoria, South Africa). We opted for 20 million reads per sample for 300 cycles, yielding 150bp paired-end reads and conducting Sequencing by Binding (SSB) on the PacBio Onso platform (California, United States).