GSM9050651: S2,TMZ,Rep2; Homo sapiens; OTHER
Multiple pellets consisting of 3 million cells were collected for each sample and washed with PBS. PBS was aspirated and cells were flash-frozen and stored at -80°C. gDNA was extracted with Blood and Tissue kit (QIAGEN) according to the manufacturer's protocol. sgRNA sequences were amplified using NGS Prep Kits from CELLECTA according to the manufacturer's protocol (NGS Prep Kit for sgRNA, shRNA, and DNA Barcode Libraries; version 1).