P0h1 (CRX825203; CRA012909)
Total RNA was subjected to mRNA isolation and fragmentation, synthesis of double-stranded cDNA, purification of double-stranded cDNA, cDNA fragmentation modification, library amplification, etc. The amplified products of the library were recovered with 2 % agarose gel. After the use of TBS380 quantitative on the machine ; bridge PCR amplification was performed on cBot to generate clusters ; finally, the sequencing library of the Illumina platform was performed.