RNA-seq for Allan knockout of P. Berghei at 30 min, Replicate 2
Libraries were prepared from lyophilized total RNA, starting with the isolation of mRNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB), followed by the NEBNext Ultra Directional RNA Library Prep Kit (NEB) as per the manufacturers instructions. Libraries were amplified through 12 PCR cycles (12 cycles of [15 s at 98C, 30 s at 55C, 30 s at 62C]) using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems). Sequencing was performed on a NovaSeq 6000 DNA sequencer (Illumina), generating paired-end 100-bp reads.