mNGS Joint 4 (CRX2027801; CRA030534)
The library preparation for metagenomic sequencing on Huada's MGISEQ-2000 involves extracting high-quality DNA, fragmenting it, repairing ends, adding A-tails, ligating adapters, amplifying via PCR, purifying to remove impurities, quantifying and assessing quality, normalizing, pooling if multiple libraries, diluting to the appropriate concentration, and finally loading onto the sequencing platform. submitted_instrument_model: MGISEQ-2000