GSM9777695: GSK3B wild-type RKO RNA-Seq 3; Homo sapiens; RNA-Seq
Total RNA was isolated using TRIzol reagent (Invitrogen, CA, USA) following the manufacturer's guidelines. The purity and concentration of the RNA were measured with a NanoDrop 2000 spectrophotometer (Thermo Scientific, USA). RNA integrity was evaluated using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Subsequently, libraries were prepared with the VAHTS Universal V6 RNA-seq Library Prep Kit in accordance with the manufacturer's instructions. The sequencing of the RNA-Seq libraries was carried out on an illumina X10 platform, generating 150 bp paired-end reads.