GSM9251116: RNA-Seq,skeletalmuscle-hiit5; Mus musculus; RNA-Seq
~20–30 mg gastrocnemius homogenized; total RNA extracted with Quick-RNA™ Miniprep Plus (Zymo). Quantity/purity by NanoDrop; integrity by Agilent TapeStation. 100 ng total RNA used for QuantSeq 3′ mRNA-Seq Library Prep Kit FWD v2 (Lexogen): oligodT priming, second-strand synthesis by random priming, bead cleanup, PCR barcoding. Libraries QC'd on TapeStation and sequenced on Illumina NovaSeq X Plus (SE75).