GSM9159056: Short TFR-patient 1 Relapse post-DC; Homo sapiens; OTHER
RNA was isolated from the patients' PBMCs using the RNeasy plus micro kit (Qiagen 74034). Nested PCR was performed to enrich the bcr-abl kinase domain (KD) with bcr-abl specific primer set 1 (bcr-F: ACAGCATTCCGC TGACCATCAATAAG; abl-R1: ATGGTCCAGAGGATCGCTCTCT) and abl kinase domain primer set 2 (abl-F:  TGGTTCATCATCATTCAACGGTGG; abl-R2: TCCACTTCGTCTGAGATACTGGATT). The library was constructed with Nextera XT DNA Library Prep kit( Illumina, FC-131-1024  ) and purified with SPRIselect beads (Beckman Coulter, A66514). The multiplexed libraries were loaded for next-generation sequencing (NGS) on Illumina HiSeq 4000 using single-ended 50bp mode.