GSM9290794: Medial preoptic area, 50 ug/kg Mixed BP, Male, rep3; Rattus norvegicus; OTHER
RNA was extracted using the MagMAX FFPE DNA/RNA Ultra Kit (#A31881, ThermoFisher Scientific). For unfixed frozen tissue, 210 μl of protease solution was pipetted onto each MPOA sample and allowed to incubate at 55C at 900 rpm for at least one hour. The homogenate was immediately processed for RNA and DNA extraction using the Kingfisher Flex System following the manufacturer's instructions. All RNA samples were diluted to 20 ng/μl before submission for library preparation and tag-seq at the Genome Sequence and Analysis Facility at the University of Texas at Austin.