GSM8678780: ESC, CEBPa induced, 24h, clone 1; Mus musculus; RNA-Seq
Two clones were used for RNA sequencing as biological replicates. Total RNA was extracted using RNeasy Mini Kit (74104, Qiagen) Libraries were prepared with a TruSeq Stranded mRNA Library Preparation Kit (20020595, Illumina) and assessed using Bioanalyzer DNA 1000 (5067-1504, Agilent). Libraries were sequenced on a HiSeq2500 instrument (Illumina), obtaining at least 40 million reads per sample.