WGS of Klebsiella pneumoniae
Genomic DNA libraries are prepared using the Nextera XT Library Prep Kit (Illumina, San Diego, USA) following the manufacturers protocol with the following modifications: input DNA is increased 2-fold, and PCR elongation time is increased to 45 seconds. Libraries are sequenced on an lllumina NovaSeq 6000 (Illumina, San Diego, USA) using a 250 bp paired end protocol.