RNA-seq of butyrate micelle or control treated murine Ly6Chi CD11b+ myeloid cells
RNA-seq of butyrate micelle or control treated murine Ly6Chi CD11b+ myeloid cells
Live CD11b+ Ly6Chi cells were FACS sorted from wildtype B6 mice into Takara RNA isolation sort buffer containing RNase inhibitor and 3' Primer II A (as per Takara instructions)  RNA was isolated from sorted cells using the Takara SMART-Seq mRNA LP kit according to manufacturer directions.  RNA libraries were constructed using Takara SMART-Seq mRNA LP kit and Takara unique dual index kit according to manufacturer directions. Libraries were quantified, then equal quantity of library was pooled before sequencing