GSM8754044: Halofuginone treated CEBPG KO cells, rep 1; Mus musculus; RNA-Seq
RNA was isolated with the Zymo Quick-RNA MicroPrep Kit (Zymo Research, #R1050) with DNase I treatment according to the manufacturer's instructions. Samples were checked for quality using a tapestation. Messenger RNA was purified from total RNA using poly-oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers. Then the second strand cDNA was synthesized using dUTP, instead of dTTP. The directional library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. After library quality control, different libraries were pooled based on effective concentration and targeted data amount.