RNA-seq of wild type Pseudomonas protegens CHA0 and H-NS-like mutants ΔmvaT, ΔmvaV and ΔmvaTΔmvaV
RNA-seq of wild type Pseudomonas protegens CHA0 and H-NS-like mutants ΔmvaT, ΔmvaV and ΔmvaTΔmvaV
Pseudomonas protegens CHA0 is type strain widely used as an environmental model.  The RNA was extracted from wild type CHA0 and mutants defective for the H-NS-like regulators MvaT and MvaV (ΔmvaT, ΔmvaV and ΔmvaTΔmvaV). Cultures of the strains were restarted from overnights into fresh NYB at a ratio of 1:100. Once the cultures reached the exponential growth phase (OD600nm of 0.4-0.6), they were either induced with mitomycin C (9 nM) or hydrogen peroxide (10 mM) or were not induced. After 3 h of induction, cells were treated with RNAprotect Bacteria Reagent (Qiagen). Sample were centrifuged and the pellets were flash-frozen in liquid nitrogen and stored at -80 °C. The RNeasy Mini Kit (Qiagen) was used to extract the RNA and purified using the RNAeasy kit (Qiagen) following TurboDNase treatment (ThermoScientific) removing potential DNA contamination. The libraries were prepared using the Illumina Stranded mRNA kit (Illumina). SPRI beads were used to purify the final library at a 0.9X ratio and quantified using Qubit (Thermo Scientifics). A fragment analyzer (Agilent) was use to analyze the size pattern of the final library.