GSM8752875: Vehicle, replicate 3; Mus musculus; ssRNA-seq
Total RNA was isolated using the RNeasy Plus Mini Kit (Qiagen). Total RNA was quantified with the Qubit RNA HS Assay Kit (Thermo Fisher Scientific) and quality was assessed using RNA ScreenTape on TapeStation 4200 (Agilent Technologies). RNA-seq libraries were prepared using the Truseq Stranded mRNA Kit (Illumina). Libraries were quantified with the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific) and the average library size was determined using D1000 ScreenTape on TapeStation 4200 (Agilent Technologies). Libraries were pooled and sequenced on a NovaSeq 6000 (Illumina) to generate 30 million single-end 50-base pair reads for each sample.