16S amplicons: reef biofilm month 6
Genomic DNA was extracted using DNeasy PowerBiofilm Kits following the manufacturers protocol. Following extraction, genomic DNA was sent to the Microbiome Core at the University of Michigan for amplicon sequencing of the V4 region of the 16S rRNA gene of bacteria and archaea (515F-Y, 5-GTG YCA GCM GCC GCG GTA A-3', Parada et al. 2016; 806R, 5-GGA CTA CNV GGG TWT CTA AT-3', Apprill et al. 2015) with an Illumina MiSeq 500v2 Reagent Kit and a 2x250 paired end configuration.