GSM9100619: HCT116, wtFTO, 0 Dt, rep1; Homo sapiens; OTHER
Cells were harvested and total RNA was extracted using TRIZOL reagent (Invitrogen) following the manufacturer's protocols. To isolate mRNA from total RNA, the Dynabeads® mRNA Purification Kit (Invitrogen) was used following the manufacturer's protocols. The RNA concentration was determined using Nanodrop (Invitrogen) by measuring the UV absorbance at 260 nm. RNA libraries for RNA-seq were prepared using VAHTS Stranded mRNA-seq Library Prep Kits (Vazyme) following manufacturer's protocols.