GSM8625269: meRIP_m6A_DMSO; Homo sapiens; RIP-Seq
RNA  was extracted using Trizol (Ambion, 15596926) following the instructions provided by the manufacturer. mRNA was purified using GenElute mRNA Miniprep Kit (Sigma, MRN70-1KT) following the manufacturer's instructions. Then, mRNA purified was treated with TURBO DNase (Invitrogen, AM2238) following the manufacturer's protocol. After that, mRNA was precipitated. To perform the meRIP, it was used the EpiQuik ™ CUT&RUN m6A RNA Enrichment (MeRIP) Kit (EpigenTek, P9018-12). The libraries for meRIP-seq were done following the manufacturer's protocol of the EpiNext™ CUT&RUN RNA m6A-Seq Kit (EpigenTek, P9016-12) and a spike-in from yeast was used as a control to normalize meRIP sequencing.