GSM9292356: Gata2cKO-2 Mesonephros E16.5; Mus musculus; RNA-Seq
RNA was harvested using PicoPure RNA Isolation Kit from ThermoFisher Scientific cat#KIT0204 mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.