WES of homo sapiens: Melanoma biopsy collected between weeks 3-5 after the start of therapy (ontx)
DNA was extracted from biopsies (stored in formalin-fixed paraffin-embedded blocks or slides) using the Covaris truXTRAC FFPE tNA Ultra kit. Whole exome sequencing libraries were generated from 100ng of genomic DNA on the Agilent BRAVO platform using the Agilent SureSelect XT HS2 DNA library preparation kit with the Agilent SureSelect Exome v7 capture probe (spiked with equimolar concentration of the Agilent OneSeq Backbone probes tiling the whole genome at 300kb resolution). Libraries were pooled for a final concentration of 2nM and sequenced on the Illumina NovaSeq X platform (S4 flow cell, 2x150bp reads). Data is proved in the form of raw fastq files.