WGS of sludge
The raw sequencing reads were subjected to quality control and filtering to obtain clean reads for subsequent bioinformatics analysis. The clean reads were then assembled, coding genes were predicted, and a non-redundant gene set was constructed. Functional annotation and taxonomic analysis of the non-redundant gene set were performed using both general and specialized databases. Species composition and abundance information of the samples were also calculated. The final analysis report was completed with the support of BMKCloud (http://www.biocloud.net/).