Endophytic bacterial metagenome of Pokkali rice
PCR amplification using V3F and V4R primers was performed in 20l reactions with EmeraldAmp GT PCR Master Mix on a BIORAD thermal cycler, following standard cycling conditions. Amplicons (~500bp) were verified on 2% agarose gel and purified using bead-based cleanup. Libraries were prepared using NEBNext Ultra DNA Library Prep Kit, quantified via Qubit 4.0 and Bioanalyzer 2100. Sequencing was carried out on the Illumina MiSeq platform, generating 250bp paired-end reads.