AsCas12a CLIP-seq library from psiMIX and AsCas12a co-tranfected HEK295T cells
Immunoprecipitation was performed using the AsCas12a antibody from Cell Signaling Technology (E1U7C). Libraries were prepared with NEBNext Ultra II Directional RNA Library Prep (NEB #E7765S). Indexes used were NEBNext Multiplex Oligos for Illumina