16s amplicon for mangrove soil
The V3V4 hypervariable regions of the bacterial 16S rRNA gene were amplified using primers 338F (5-ACTCCTACGGGAGGCAGCAG-3) and 806R (5-GGACTACHVGGGTWTCTAAT-3) (Bates et al., 2011). PCR amplification was performed under the following thermal regime: an initial denaturation at 98C for 1 min; followed by 30 cycles of denaturation at 98C for 10 s, annealing at 50C for 30 s, and extension at 72C for 30 s; and a final extension at 72C for 5 min (Fang et al., 2024). Purified PCR products were subsequently sequenced using a 2  323 bp paired-end strategy on the Illumina MiSeq platform (Illumina, San Diego, CA, USA) at Majorbio Bio-Pharm Co., Ltd (Shanghai, China).