Oxford nanopore reads of full-length cDNA of ANK3 extracted from frontal cortex and amplified from TSS1
ANK3-specific amplification was performed with 28x13 minute long extension cycles using a time increase per subsequent cycle (+20 seconds) using primers designed for either transcription start site 1 (TSS1) or TSS2 together with a common reverse primer. Human primers: ANK3_TSS1=GCACCCGTAAATGGACTTTGG, ANK3_TSS2=GCCTGATTAGAGGTGCCTGA, ANK3_R=TCCTGCTGACATTTCTTCCAC