RNA-Seq of Arabidopsis root C24 1/2 MS + 30 mM KCl + 75mM NaCl
Total RNA was isolated using Trizol [29], and mRNA was enriched using Ambion Dynabeads. mRNA was used for library preparation, and cDNA libraries were sequenced, producing 150 bp paired end-reads (NovaSeq6000, Novogene) with a data output average of 15 million reads per sample and Q30 of 85%.