kidney of rat
The single-cell suspension was adjusted to a final concentration of 700-1200 cells/L. Approximately 10,000 viable cells per sample were loaded onto a 10x Genomics Chromium Controller using the Chromium Next GEM Single Cell 3' Reagent Kit v3.1 , with a target capture of 8,000 cells. Single-cell cDNA synthesis, amplification, and library construction were performed according to the manufacturer's standard protocol. The generated libraries were sequenced on an Illumina NovaSeq 6000 platform using a PE150 (paired-end 157 bp) sequencing strategy. All library preparation and sequencing services were provided by LC-Bio Technology Co., Ltd. .