WGS of Oryza sativa : MMR35B2-tohkondi
Sheared DNA was converted into double-stranded DNA libraries with unique dual indexes using tagmentation with an in-house protocol for the Illumina DNA Prep kit (formerly Nextera DNA Flex). Our in-house protocol is based on a previously published hack protocol of Gaio et al., (2022, Microb Genom) but we added normalization steps before and after tagmentation. The library size distribution (average insert size ~450 bp) was checked using the  Agilent Technologies 5200 Fragment Analyzer.