HiSeq X Ten paired end sequencing of SAMD00761969
Total RNA was extracted from flesh hairy roots frozen in liquid nitrogen followed by DNase I treatment, column purification, and cDNA synthesis as described previously(Suzuki et al. 2019). Messenger RNA was purified from 1 microgram of total RNA following previous study(Townsley et al. 2015). Complementary DNA libraries were prepared using KAPA Stranded RNA-Seq Library Preparation Kit (Kapa Biosystems, Wilmington, MA) according to the manufacturer?s instructions.