Hi-C assembly raw data for Saussurea hypsipeta
The Hi-C mounting method is based on the principle of three-dimensional genomic spatial interactions, integrating experimental library construction, high-throughput sequencing, and bioinformatics analysis processes to achieve chromosome-level genome assembly. The experimental design begins with the cross-linking and fixation of chromatin to establish its three-dimensional structure. Chromatin is digested using the DpnII restriction endonuclease (recognizing the GATC site) to generate sticky-ended fragments. After biotin labeling, spatially adjacent DNA fragments are captured via blunt-end ligation to form chimeric molecules. A PE150 sequencing library is then constructed. The raw sequencing data (FASTQ format) is filtered using fastp for quality control, removing adapter sequences, reads containing N bases, and low-quality reads, retaining high-quality data for subsequent analysis. hypotonic buffer (10 mM Tris-HCl, 10 mM NaCl, 0.2% NP-40) to isolate nuclei, followed by quality assessment via spectrophotometry (A260/A280 > 1.8).