GSM8595560: LF1 cells, late senescence, replicate 4; Homo sapiens; RNA-Seq
PRO, ESEN and LSEN cells were harvested using Trizol. Total RNA was then extracted using a Qiagen Rneasy column kit. Libraries were then prepared using NEBNext® Ultra™ II Directional RNA Library Prep with Sample Purification Beads to purify mRNA (using polyA isolation oligodT beads). The samples were then sent for sequencing to Azenta, where they were all sequenced in the same lane. NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®