16s rRNA amplicon sequencing of wastewater
Extract DNA by using DNeasy PowerSoil Pro Kit (Qiagen), and amplify V3-V4 region of 16S rRNA with 341F (5-CCTACGGGNGGCWGCAG-3) and 805R (5-GACTACHVGGGTATCTAATCC-3) primer sets on a MiSeq platform (Illumina)