GSM9815001: female, high fat diet, replicate 1; Mus musculus; RNA-Seq
Total RNA was isolated from approximately 20 mg of homogenized whole-liver powder using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. 1 µg of total RNA per sample was submitted to Azenta Life Sciences for library preparation using the NEBNext Ultra RNA Library Prep Kit (New England Biolabs) followed by sequencing on the NovaSeq platform (Illumina).