16S rRNA amplicon sequencing of Medicago truncatula root microbiota under germ-free conditions (cerk1 genotype)
Root samples were harvested and total genomic DNA was extracted using the FastDNA SPIN Kit (MP Biomedicals) following the manufacturers instructions.The bacterial 16S rRNA gene variable regions V5V7 were amplified using primers 799F and 1193R. Amplicon sequencing was performed on the Illumina HiSeq 2500 platform with a paired-end 250 bp configuration (PE250).