GSM8888293: Hi-C, i8, recombined, clone 62, replicate 1; Mus musculus; Hi-C
Hi-C was performed as in (Haarhuis et al. 2017). Cells were crosslinked using 2% formaldehyde. DNA was digested with MboI, followed by the incorporation of biotin-labeled nucleotides at the overhangs and subsequent blunt-end ligation. Streptavidin pull-down was used to enrich the ligated DNA. Hi-C sequencing libraries were constructed with the standard end-repair and A-tailing protocols.