DNA of Bacteria: Enterobacter hormaechei
Sequencing libraries were prepared using the Rapid Barcoding Kit 24 V14 (SQK-RBK114.24; Oxford Nanopore Technologies [ONT], Oxford, UK). Sequencing was performed on the MinION platform using an R10.4.1 flow cell (FLO-MIN114; ONT) for up to 72 h. Basecalling and demultiplexing were performed using Dorado version 1.1.1. Reads were quality filtered and adapter trimming using Fastplong v0.4.1 with a minimum quality score of 10 and a minimum read length of 1,000 bp. Self-error correction was performed using DeChat version 1.0.1. with default parameters.