GSM8819486: H3K4me3, liver, Fed Vps15LKO, rep2; Mus musculus; ChIP-Seq
Liver powder was fixed for 15min at RT with 1% formaldehyde and then quenched with glycine. Tissue was washed and homogenized, after which was soncated to acheieve 200-500bp fragments. DNA fragments were subject to end-repair and 3' adenylation. PCR products were enriched using Agencourt AMPure XP-medium kit. dsDNA PCR products were denatured and circularized by the splint oligonucleotide sequence. Single-stranded circular DNA was formatted as the final library, and DNA nanoballs were made from the library and loaded into a patterned nanoarray. Paired-end reads were generated by sequenced combinatorial Probe-Anchor-Synthesis with the DNBSEQ-400 sequencer.