RNA-Seq of Scylla paramamosain larvae: zoea V of control group without any treatment
After soaking in DEPC water, larvae were removed from RNA preservation solution, and total RNA was extracted using Trizol reagent (Takara Bio Inc.). RNA purity and concentration were measured using a NanoDrop2000 spectrophotometer (Thermo Scientific, USA), and RNA integrity was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Transcriptome libraries were prepared using the VAHTS Universal V5 RNA-seq Library Prep Kit according to the manufacturer's instructions.