GSM8788051: hiPSC-CMs treated with bupivacaine biol rep 2; Homo sapiens; RNA-Seq
RNA was extracted using the RNA Nucleospin RNA kit (Bioke) per manufacturer's instructions After RNA extraction, the concentration and quality of the total extracted RNA were evaluated by using the 'Quant-it ribogreen RNA assay' (Life Technologies, Grand Island, NY, USA) and the RNA 6000 Nano chip (Agilent Technologies, Santa Clara, CA, USA), respectively. Subsequently, 10 ng of RNA was used to perform an Illumina sequencing library preparation using the QuantSeq 3' mRNA-Seq Library Prep FWD Kit (Lexogen, Vienna, Austria) per manufacturer's instructions. During library preparation, 17 PCR cycles were used. Libraries were quantified by qPCR, according to Illumina's protocol 'Sequencing Library qPCR Quantification protocol guide', version February 2011. A High Sensitivity DNA chip (Agilent Technologies, Santa Clara, CA, US) was used to control the library's size distribution and quality. Sequencing was performed on a high throughput Illumina NextSeq 500 flow cell generating 75 bp single reads.