GSM8567579: GNP RNAseq P1 replicate 1; Mus musculus; RNA-Seq
GNPs and MB cells were purified using established percol fractionation protocol.   For RNAseq the cells were immediately lysed in Trizol.  Purified RNA was then DNase treated and cleaned up with an RNEasy column (Qiagen).  mRNA was purifid using NEBNext Poly A selection beads (NEB). RNAseq libararies were prepared using Illumina TruSeq v2 library preperation kit (Illumina Inc).  Sequencing was performed using HiSeq 2500 (Illumina Inc).