GSM8436113: replicate-2.snRNA-seq; Camellia sinensis; RNA-Seq
Approximately 1 g of fresh, tender tea plant root tips, specifically from 'Shuchazao' (Camellia sinensis ' Shuchazao '), were excised using a sterile razor blade and immediately placed into a pre-cooled petri dish containing 500 μl of Nuclear Isolation Buffer (NIB; Sigma-Aldrich, Catalog No. CELLYTPN1) Chromium™ Single Cell 3' Solution utilized GemCode technology within a microfluidic platform, precisely controlling the concentration of cell nuclei within the suspension between 700-1200 nuclei per microliter. cDNA was enzymatically segmented into approximately 200-300 bp fragments. Following this segmentation, standard library preparation steps were undertaken, including the attachment of sequencing adapters and primers. A final round of PCR amplification was conducted to finalize the DNA library. This library was then sequenced on the Illumina NovaSeq 6000 platform by LC-Bio Technology Co., Ltd., based in Hangzhou, China, ensuring high-throughput results.