GSM9523143: JB161; Homo sapiens; ChIP-Seq
HT-29 cells were harvested, and histones were extracted from 1 × 10⁷ cells using the EpiQuik Total Histone Extraction Kit (EpigenTek) according to the manufacturer's instructions. The resulting chromatin solution was incubated with Histone H3 (D1H2) Rabbit mAb (Cell Signaling Technology) pre-bound to Dynabeads Protein G (Invitrogen), and immunoprecipitated DNA was recovered following the kit protocol.