GSM9423148: NFs from the control mesenery_rep3; Homo sapiens; RNA-Seq
Total RNA was isolated from the cells using the XENOPURE Total RNA Purification Kit (Xenohelix, Korea) following the manufacturer's protocol. RNA integrity was assessed using the Bioanalyzer 2100 system(Agilent Technologies, CA, USA). Messenger RNA was purified from total RNA using poly-T oligo-attachedmagnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the secondstrand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantificationand bioanalyzer for size distribution detection.