RNA-seq of Culaea inconstans gill tissue: Sample #72
Frozen gill tissue was homogenized in ice-cold Qiazol using a stator and rotor homogenizer. Total RNA was precipitated from the homogenate following Qiagens manufacturers protocol. RNA was reconstituted in nuclease-free water, and RNA quantity and quality was assessed using Agilents 2100 Bioanalyzer. RNA integrity number was > 9 in all samples. Library preparation and RNA sequencing were done by Genome Quebec. mRNA stranded library preparation was done using NEBNext mRNA preparation kits, and RNA sequencing was done using NovaSeq 6000 PE100 with 25 M reads per sample.