RNA-Seq of Canuella perplexa: pooled adult sample
Total RNA from 50 pooled C. perplexa specimens per sample was isolated using the RNeasy Plus Micro Kit (QIAGEN). cDNA libraries were constructed using the NEBNext Poly(A) mRNA Magnetic Isolation and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Samples were run on an Illumina NovaSeq 6000 platform with 100 bp paired-end reads at the Cologne Centre for Genomics (University of Cologne).