GSM9290046: NCIH661_Dppa2_4_3; Homo sapiens; Bisulfite-Seq
Cells were harvested, washed once with PBS, stored in RLT+ lysis buffer (Qiagen) and snap frozen. gDNA was extracted from cells using an AllPrep RNA/DNA extraction kit (Qiagen #80204) according to the manufacturer's instructions DNA libraries were generated using the NEBNext End Prep kit (NEB E7370, E7535) and bisulfite converted using the EZ DNA Methylation Gold Kit (Zymo Research #D5005, D5006) according to the manufacturer's instructions. Libraries were amplified using KAPA HiFi Uracil+ (KK2801/2) using NEBNext Universal and Index primers, cleaned up using 0.8x Ampure beads and pooled for sequencing