GSM8595867: 293T cells, DUX4, 12h, rep2; Homo sapiens; RNA-Seq
Cells were resuspended in Trizol (Invitrogen) and RNA extracted from Trizol. RNA-sequencing libraries were constructed using commercial kits (New England Biolabs) total RNA-seq with rRNA depletion as indicated; CUT&RUN was performed according to the CUT&RUN: Targeted in situ genome-wide profiling with high efficiency for low cell numbers V.3 protocol.