GSM9599525: Neutro_HS; Homo sapiens; RNA-Seq
PMNs (5 × 10^5) per condition were resuspended in HBSS. HBSS, HS or HS-opsonized Ad5 (MOI 10^4 vp/cell) were incubated with PMNs for 30 min at 4°C. After washing with HBSS, samples were incubated 90 min at 37°C then filtered to discard aggregates. PMNs were counted with Trypan Blue to assess cell viability (that was around 80%) then transferred in DNA LoBind tubes Library was performed according to the manufacturer's instructions 10X Genomics Chromium 3' single-cell RNAseq