GSM9101199: 13_SNAT3_cKO_S8; Mus musculus; RNA-Seq
Total RNA was extracted from 9 tissue samples using the RNeasy mini kit (Qiagen, cat no. 74106). 600 ul of RLT buffer was added to each sample and the samples were disrupted and homogenized using the Polytron PT3000 Homogenizer (Kinematica). After homogenization, samples were centrifuged for 3 min at 13000g and the supernatant was transferred to a new tube for automated extraction using the QIAcube Connect (Qiagen). The RINe value of all samples was in the range of 9.7-9.9, indicating very high quality. RNA-seq libraries were constructed simultaneously using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, cat no. E7760), according to the manufacturer's protocol.