16S rRNA sequencing of prokaryotic community: alpine meadow peatland soil
Soil DNA was extracted from 1.0 g soil using the DNeasy PowerSoil Pro kit (Qiagen) and assessed with a NanoDrop ND-1000 Spectrophotometer. DNA met the required 260/230 (>1.7) and 260/280 (>1.8) ratios and was stored at -80. Microbial community structures were analyzed using 16S rRNA gene sequencing for bacteria and archaea, and ITS sequencing for fungi. PCR amplification, library preparation, and sequencing were performed by Magigene Biotechnology. The V4-V5 16S rRNA and ITS regions were amplified with specific primers. Libraries were sequenced on the Illumina Nova 6000 platform with a 2  271 paired-end kit.