GSM9777808: Vehicle-treated BMDN Input ChIP-Seq 2; Mus musculus; ChIP-Seq
The chromatin immunoprecipitation (ChIP) protocol was performed to analyze histone modifications. Briefly, cells were collected and treated with 1% formaldehyde for 10 minutes at room temperature to induce crosslinking. After sonication, an anti-H3K36me2 antibody was used for immunoprecipitation, and then the DNA was extracted and purified using the Universal DNA Purification Kit. Libraries were constructed from ChIP samples using the VAHTS Universal DNA Library Prep Kit for Illumina V3 and then sequenced on the NovaSeq X platform by Annoroad Gene Tech (Beijing) Co., Ltd.