Amplicon-Seq:2023-Sub2-4-1-D1-W-2
DNA was extracted from soil with Qiagen Dneasy PowerSoil Pro Kit, DNA target regions 16SrDNA and ITS2 were amplified in PCR with primer sets (16S: 515f, 806r and ITS2: ITS4, fITS7),  purified with AmpPure XP Beads, indexed in another PCR with Illumina Nextera XT indexing primers, purified with AmpPure XP Beads. Samples were equimolarly pooled for paired-end sequencing