RNA-seq of primary human fibroblasts after X-ray induced senescence
RNA-seq of primary human fibroblasts after X-ray induced senescence
Cell monolayers were trypsinized, neutralized with medium, washed with PBS, and centrifuged. Washed cell pellets were resuspended in Macherey-Nagel Nucleospin RNA Plus Lysis Buffer. No dissections were performed so this protocol is not applicable. MEM + 10%FBS + sodium pyruvate + non-essential amino acids + Gluta-Max + pen/strep in 5% 02 + 5% CO2 20 or 40 Gy X-rays at 120 kV generated by a Faxitron 43855-D generator The following software were used for data conversion: EdgeR Limma voomrmalization and data  RNA was extracted with the Macherey-Nagel Nucleospin RNA Plus. NEBNext Ultra II Directional RNA Library Kit with PolyA RNA selection