G sulfurreducens propionate 4
Total RNA was extracted from 12 bacterial cell pellets, and RNA integrity and potential DNA contamination were assessed prior to library preparation. An input of 200 ng of RNA was used, and rRNA was depleted using the Illumina Ribo-Zero Plus rRNA depletion kit. The mRNA libraries were prepared using the Illumina protocol and quality-checked before sequencing. Libraries were sequenced on an Illumina NovaSeq 6000 platform to generate 150 bp paired-end reads. Raw reads were demultiplexed and converted to FASTQ format using bcl-convert v4.2.4 (Illumina).