RNA Seq (V3-V4) metagenome of rose stem and leaves
High quality clean reads were obtained using Trimmomatic v0.38 to remove adapter sequences and law quality sequences along with sliding window of 20bp and minimum length of 100bp. Stiching PE data into single end reads using FLASH.  High quality clean reads the denoised and chimeric sequences were then filtered through DADA2. Taxonomic classification of amplicon sequence variants were performed with q2 feature classifier using pretrained classifier based on SILVA 138.1 database. Diversity metrices within the sample were calculated.