16S rRNA gene V3–V4 amplicon sequencing of Pekin duck jejunal contents
Bacterial 16S rRNA gene amplicon sequencing was performed on DNA extracted from jejunal-content samples of Pekin ducks. The V3–V4 region was amplified by PCR using primers 338F (5′-ACTCCTACGGGAGGCAGCA-3′) and 806R (5′-GGACTACHVGGGTWTCTAAT-3′). Libraries were sequenced on an Illumina NovaSeq platform using paired-end 250-bp reads.