sequences of gene
Cells were isolated from peripheral blood mononuclear cells (PBMCs) to obtain a single-cell suspension.The cell suspension was loaded into a microfluidic device and single-cell separation was completed according to the Poisson distribution principle. Poly(A) tails were captured using magnetic beads labeled with molecules. The cells and mRNA were labeled after cell lysis. mRNA was collected from the magnetic beads in the chip and reverse transcribed into complementary DNA (cDNA) for amplification. After fragmentation and splicing of the local cDNA, a transcriptome sequencing library suitable for the Illumina sequencing platform was constructed. The library was sequenced on the Illumina Nova 6000.