GSM9669510: Anti-IL17A-Ab treated 1, BM; Mus musculus; RNA-Seq
Total RNA was isolated from bone marrow (BM) cells and adipose tissue macrophages (ATMs) collected from vehicle control and three treatment groups (n = 3 mice per group) using the Qiagen RNeasy Plus Micro Kit, following the manufacturer's instructions. RNA quality was assessed using an Agilent Bioanalyzer, and samples with RNA integrity number (RIN) > 8 were used for downstream library preparation. Paired-end RNA-seq libraries were prepared at the Indiana University Genomics Core Facility using standard Illumina protocols. Sequencing was performed on an Illumina NovaSeq X Plus platform. Each experimental group included three biological replicates, with approximately 30 million reads generated per sample. Library type - Unstranded (RSeQC: 1++,1--,2+-,2-+; 1+-,1-+,2++,2--)