GSM9293048: A549, ZIKV, 32h, rep3; Homo sapiens; RNA-Seq
After the cells were gently washed 3X with PBS, cell lysates were harvested at 16 and 32 h, after addition of 1 ml ice-cold TRI-reagent (Invitrogen Cat# AM9738) and total RNA was extracted following the manufacturer's protocol. RNA-seq libraries were constructed using 1 µg of total RNA obtained from each of the samples per condition, after employing ribosomal RNA depletion and poly(A) selection methods using the TruSeq Stranded mRNA Library Prep Kit (Illumina Cat# 20020595).