16S rRNA gene amplicon sequencing of Gossypium hirsutum: rhizosphere soil microbiota under Con, WT and KO treatments
Total microbial DNA was extracted from cotton rhizosphere soil. The V4 region of bacterial 16S rRNA gene was amplified by PCR using 515F and 806R primers. Amplicon libraries were constructed, qualified and sequenced on the Illumina NovaSeq 6000 platform with paired-end mode.