Horse adipose tissue-derived mesenchymal stem cells
Total RNA was extracted from cells or EVs using TriReagent following a modified protocol optimized for low input amounts (for EVs). Libraries were constructed using the NEBNext Multiplex Small RNA Library Prep Set for Illumina, adhering to the manufacturers instructions. Sequencing was performed on a NovaSeq 6000 system (Illumina, USA) at The OMRF Clinical Genomics Center (CGC) (Oklahoma, USA) with a read length of 2  150 bp, but only the first 75 bases of read one were utilized for downstream analysis.