DNA-seq of Paenibacillus sp.
Whole-genome sequencing was performed using the service provided by MicrobesNG, (Birmingham, UK), using the following protocol: genomic DNA libraries were prepared using the Nextera XT Library Prep Kit (Illumina, San Diego, USA) following the manufacturers protocol with the following modifications: input DNA is increased 2-fold, and PCR elongation time is increased to 45 seconds. DNA quantification and library preparation are carried out on a Hamilton Microlab STAR automated liquid handling system (Hamilton Bonaduz AG, Switzerland). Libraries are sequenced on an lllumina NovaSeq 6000 (Illumina, San Diego, USA) using a 250 bp paired end protocol. Reads were adapter trimmed using Trimmomatic version 0.30 (https://academic.oup.com/bioinformatics/article/30/15/2114/2390096) with a sliding window quality cutoff of Q15.