GSM8990513: passage15 batch 3; Homo sapiens; RNA-Seq
Total RNA was extracted from cultured hDFs using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. RNA quality was assessed with a Nanodrop 2000 (Thermo Fisher Scientific) and Agilent 4000 TapeStation. Only samples with RIN > 10 were used for sequencing. RNA libraries were prepared using the QuantSeq 3' mRNA-seq Library Prep Kit FWD for Illumina (Lexogen) following the manufacturer's instructions. Sequencing was performed using the Illumina NextSeq 550 platform with single-end 75 bp reads.