GSM8818949: 241-Pax5-leukemic, B-cell leukemia, in vitro culture 2; Mus musculus; ATAC-seq
200,000 cells of each cell population analyzed were sort-purified and frozen in 90 % FCS and 10 % DMSO. Cryopreserved cells were sent to Active Motif to perform ATAC-seq. The cells were thawed in a 37°C water bath, slowly diluted with RPMI media, pelleted, washed with cold PBS, and tagmented with a Tn5 enzyme. Briefly, cell pellets were resuspended in lysis buffer, pelleted, and tagmented using the enzyme and buffer provided in the ATAC-Seq Kit (Active Motif). Tagmented DNA was then purified using the MinElute PCR purification kit (Qiagen), amplified with 10 cycles of PCR, and the resultant libraries were purified using Agencourt AMPure SPRI beads (Beckman Coulter).  Resulting material was quantified using Qubit (Invitrogen) and TapeStation (Agilent). Final libraries were sequenced with PE50 sequencing on the Illumina platform.