GSM9521874: HSC, MCD; Homo sapiens; RNA-Seq
Cells were lysed in RNA lysis buffer and total RNA was isolated using PureLink RNA Mini Kit following manufacturer's instructions (Life Technologies). RNA quality was assessed by Agilent 4200 TapeStation System. Sequencing libraries were prepared in biological replicates from polyA enriched mRNA. RNA-seq libraries were prepared from poly(A)-enriched mRNA as previously described (PMID:31761566). Libraries were then size selected by beads selection, quantified using a Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific), and sequenced on a Nextseq 2000 system at the Genome Sequencing Facility of the University of Texas Health Science Center at San Antonio.