RNA-Seq of  colorectal cancer:colorectal cancer tissue
The total RNA concentrations were determined using a NanoDrop ND-2032 spectrophotometer (Thermo Scientific, UK). cDNA synthesis and multiplex PCR amplification of all possible rearranged TCR -chain sequences were performed together using Immune Repertoire Library Preparation Kits (Geneway, Jinan, China) using a previously described protocol.