GSM8752849: NGP_input; Mus musculus; ChIP-Seq
Cells were fixed in 1% formaldehyde for 15 min,after quenching and cell lysis,chromatin was sonicated using a Sonics VCS130 The high-throughput DNA sequencing libraries were prepared by using VAHTS Universal DNA Library Prep Kit for Illumina V3, the 10% DNA was used as starting material for input and 80% for IP samples. The library products corresponding to 200-500 bps were enriched, quantified and finally sequenced on Illumina Novaseq 6000 with PE150 model.