GSM9422661: Pd biol rep 3; Drosophila melanogaster; RNA-Seq
Guts were dissected from virgin female flies and immediately placed into RNAlater (Invitrogen). Total RNA was isolated using Trizol (Thermo Fisher) with glycogen to enhance yield. RNA was quantified using a Fragment Analyzer (Agilent). Fifty ng of total RNA was used for library construction. RNA libraries were prepared using Stranded Total RNA Prep with Ribo-Zero Plus kit (Illumina) following manufacturer's protocols.