Illumina barcode sequencing SARS-CoV-2 Nucleocapsid gfp positive myc positive population
Cells washed once with PBS and RNA extracted using the GeneJet RNA purification kit. cDNAs prepared with High-Capacity cDNA Reverse Transcription Kit. Custom primers were designed to anneal immediately downstream of the barcode sequence. Barcodes were then amplified by 8 rounds of PCR using Platinum SuperFi DNA Polymerase and amplicons were purified using the QIAquick PCR Purification Kit.