GSM8787820: DLD-1 LB2-AID, 16hr auxin, replicate 3; Homo sapiens; RNA-Seq
DLD1 parental, AID-Lamin A/C, AID-Lamin B1, and AID-Lamin B2 cells were treated with auxin for 0, 16 or 96 hours. Four replicates per condition were used. Each replicate was one well of a 6-well plate (Corning 3516). RNA was extracted using RNeasy Plus Mini Kit (QIAGEN 74136). 500ng of RNA was used for the construction of sequencing libraries. The libraries were prepped using the Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus kit (Illumina 20040529) following the standard protocol. Each library was given a unique index using IDT for Illumina – DNA/RNA UD indexes Set A (Illumina 20026121). The library was pooled in equimolar amounts. Paired-end sequencing on the pooled library was done using the Illumina NextSeq2000.