Short description that will identify the dataset on public pages. A clear and concise formula for the title would be like: {methodology} of {organism}: {sample info} e.g. RNA-Seq of mus musculus: adult female spleen
Genomic DNA is fragmented using sonication, followed by chemical repair of DNA ends. Illumina sequencing primers are ligated to the ends, and PCR amplification is performed. Subsequently, amplified DNA fragments are screened via gel electrophoresis, retaining fragments of the target length for purification. The purified DNA fragments are then inserted into a vector. The library is transformed into E. coli via electroporation. After screening and amplification, the library construction is completed. Upon passing quality control, the resulting genomic library can be used for high-throughput sequencing to obtain the organism's complete genome sequence information.