DAM_Nup153
DamID samples were processed according to Marshall et al., Nat Protoc, 2016 (PMID: 27490632) and subjected to Illumina library preparation using the NEBnext Ultra II DNA library preparation chemistry (New England Biolabs, E7645L). The libraries were size-selected with Seramag beads. The resulting libraries were checked for quality and quantified using a Tapestation and Qubit. The final libraries were subjected to 50bp paired-end sequencing on the Illumina NovaSeqX platform. Reads from Dam-only libraries with lower read-depth were pooled into a single Dam-only control for each of the 4 Dam-Nup153 replicates.