GSM9251065: skeletalmuscle-mct5; Mus musculus; miRNA-Seq
~20–30 mg gastrocnemius was homogenized in lysis buffer; total RNA was isolated using Quick-RNA™ Miniprep Plus Kit (Zymo Research) following the manufacturer's instructions. RNA quantity and purity were assessed by NanoDrop; integrity was inspected on Agilent TapeStation. 100 ng total RNA per sample was used to prepare small-RNA libraries with NEXTFLEX® Small RNA-Seq Kit v4 (PerkinElmer). 3′/5′ adapters were ligated, cDNA synthesized, bead-cleaned, and PCR-barcoded (17 cycles). Libraries were QC'd on TapeStation, pooled, and sequenced on Illumina NovaSeq X Plus (single-end 75 bp).