Flex RNA-seq of COLO320DM and COLO320HSR: xenografts
COLO320DM and COLO320HSR xenograft samples were fixed and dissociated following 10x Genomics protocols, with tissue stored at -80C after fixation and glycerol supplementation. Fixed samples were enzymatically dissociated using Liberase, then processed using the 10x Genomics Chromium Fixed RNA Profiling workflow with custom probe hybridization. Libraries were generated targeting 60,000 cells, quality-checked, and sequenced on Illumina NovaSeq X with paired-end configuration (28-10-10-90 bp).