DNA sequencing of P. aeruginosa PAO1
DNA samples are firstly fragmented by sonication to generate fragments of 200 to 500 bp. Sequencing adatpers are ligated to these fragments in order to bind onto flow cell. These DNA will form different DNA clusters by bridge PCR amplification in order to amplify the signals. During sequencing, a single-labeled dNTP is added to the nucleic chain and release fluorescent signal, which can be captured and transferred into nucleotide.