GSM9289040: SEN1 (wild type), 2hr after Spo medium, Rep1; Saccharomyces cerevisiae; RNA-Seq
Total RNA was extracted using the RiboPure RNA Purification Yeast kit (Cat#: AM1926; Life Technologies). Total RNA (12.5 ug) was subjected to ribosomal RNA (rRNA) depletion using magnetic beads coated with antisense probes targeting the 25S, 18S, 5.8S, and 5S rRNAs. The resulting rRNA-depleted samples were purified using RNAClean XP beads, followed by polyadenylation with the E.coli Poly(A) Polymerase. Reactions were quenched using EDTA and purified again with the RNAClean XP beads before proceeding to library preparation using the Oxford Nanopore Techonlogy direct RNA-sequencing kit, according to the manufacturer protocol.