Illumina MiSeq paired end sequencing of SAMD01697856
Two-step PCR targeting ITS2 with primers ITS-S2F (5'-ATGCGATACTTGGTGTGAAT-3') and ITSp4 (5'-CCGCTTAKTGATATGCTTAAA-3'). Amplicons (~450 bp) were purified with AMPure beads. Illumina adapters/indexes were added in the second PCR. Libraries were pooled and sequenced on MiSeq paired-end 2×300 bp (read length 300 nt).