GSM8679386: NEUROD1 ASCL1: HES1 promoter driving ASCL1, NEUROD1, and GFP; Homo sapiens; RNA-Seq
Retinospheres were dissociated into single cells as previously described (Wohlschlegel, 2023). Following dissociation cells were passed through a 35 µm strainer. Fluorescent Activated Cell Sorting (FACS) was performed to isolate the GFP+ cells using a BD FACSAria III cell sorter (BD Bioscience). Following FACS purification, GFP+ cells were centrifuged at 300g at 4ºC for 5 mins. For each sample, cells were resuspended in RDM5% to reach a concentration of 1000 cells per µl. Libraries construction were done using the Chromium Next GEM single Cell 3' Reagent kits v3.1 (Dual Index) following the manufacturer's instructions. Libraries were next sequenced using Illumina NextSeq.