scRNA-Seq of mouse ovary
The nuclei of ovarian cells from 48-week-old Il11ra1-/- mice and wild-type mice were isolated using a slightly revised 10 x Genomics Chromium Single-Cell 3' kit as per manufacturers protocol. cDNA sequencing libraries were prepared according to the manufacturers protocol and sequenced on an Illumina Novaseq 6000 (paired-end multiplexing run, 150bp).