RNA Sequencing of Testicular Spermatids in Mex3d Knockout and Wild-Type Mice
RNA Sequencing of Testicular Spermatids in Mex3d Knockout and Wild-Type Mice
Round and elongated spermatids were isolated and purified from adult mouse testes. mRNA was enriched using mRNA Capture Beads, followed by high-temperature fragmentation.  The first strand of cDNA was synthesized in a reverse transcription mixture, and the second strand was synthesized while performing end repair and adding an A-tail. 