GSM8753026: 29_29-HCT116-tet-D350A-Nutlin-replicate-2; Homo sapiens; RNA-Seq
RNA was harvested using Rneasy mini plus kit (Qiagen). Libraries for 3' mRNA-seq were generated from 100ng of DNase treated total RNA using the QuantSeq FWD Library Preparation kit (Lexogen, Vienna, Austria), according to manufacturer's directions.  Overall library size was determined using the Agilent Tapestation and the DNA 5000 Screentape (Agilent, Santa Clara, CA).  Libraries were quantitated using real-time PCR (Kapa Biosystems, Wilmington, MA)