GSM8482486: KO_D0_a; Homo sapiens; RNA-Seq
The WT, KO, and ΔATG hESCs and trilineage differentiated cells were collected and RNA was extracted in accordance with Qiagen RNeasy Mini (Qiagen #74106) protocol with 650 uL lysis buffer. Libraries were prepared in accordance with Illumina Stranded mRNA Prep, Ligation (Illumina #20040534) protocol using 100 ng RNA input.