ONT sequencing of pSM620 after 72h serial culture
No specific library selection or enrichment was performed. Plasmid DNA was used directly for library preparation using Oxford Nanopore native ligation chemistry without PCR amplification or size selection. Sequencing performed on PromethION R10.4.1 flow cell. Basecalling performed with Guppy super accuracy model v4.3.0 (dna_r10.4.1_e8.2_400bps_sup@v4.3.0).