DC group 3
Metagenomic DNA was extracted from mouse fecal samples, checked for quality evaluation. Purified DNA was amplified for 16S rRNA 16sF and 16sR primers. Libraries were purified using Ampure beads and quantitated using Qubit dsDNA High Sensitivity assay kit. Sequencing was performed using Illumina Miseq with 2x300PE v3-v4 sequencing kit.