Phalangium opilio whole genome sequencing
HMW genomic DNA was extracted and fragmented to appropriate sizes. Then DNA fragments were damage repaired, end-repaired, and A-tailed. The SMRTbell library was produced by ligating universal hairpin adapters onto double-stranded DNA fragments. After the exonuclease and AMPure PB beads purification steps, sequencing primer was annealed to the SMRTbell templates, followed by binding of the sequence polymerase to the annealed templates.