Mock_6dpi_R3
Fifteen-day-old wheat plants from the cultivar Titlis were spray-treated(1 mL/plant) with a mock solution (0.1% v/v Tween 20). Upon infection, plants were placed in sealed plastic bags for 48 h to maintain high humidity. Total RNA was extracted using the TRIzol reagent (Invitrogen, Cat. No. 15596018), treated with DNase I (Qiagen, Cat. No. 792256) and purified using the RNeasy Plant Mini Kit (Qiagen, Cat. No. 74904). RNA quality check, library preparation (non-stranded polyA enrichment), and subsequent sequencing were performed by Novogene (Beijing, China)