GSM9558154: WT#5(TCRseq); Mus musculus; OTHER
10⁶ CD8⁺ T cells were sorted from lymph nodes and spleens of WT, β5i-KO, β5t-KO and BIT mice (9–12 weeks old, male, n = 4–5 individual mice per group, >98% purity.Total RNA was prepared from sorted cells with the RNeasy Kit (Qiagen). The quality of RNA was assessed by Bioanalyzer (Agilent). The library was made by using the SMARTer® Mouse TCR a/b Profiling Kit (Takara)