GSM9229730: scRNAseq, HCMV-infected fibroblasts; Homo sapiens; RNA-Seq
For single cell RNA sequencing, confluent HFF cells were infected with HCMV in T175 cm2 cell culture flasks. 6 hpi, cells were harvested and washed following instructions of the scRNAseq 10x Genomics Sample Preparation Demonstrated Protocol. From the cell suspension, 10000 live cells were subjected to single cell library preparation with a target cell count of 6000. Libraries were generated with the 10x Chromium Single Cell 3' Gene Expression v3 kit (10x Genomics) according to the manufacturer's instructions.