GSM9289682: basal_layer_ue_ls_pt06; Homo sapiens; OTHER
Prior to immunohistochemical staining, sections were deparaffinized and then rehydrated in a series of graded ethanols. Subsequently, antigen unmasking was accomplished using heated Tris-ethylene glycol tetraacetic acid buffer (pH 9) at pre-boiling temperature. The antibodies used were CD3, CD4, CD8, CD103, CD207, RORγt, FOXP3, Ki67, and MPO. Library preparation was carried out according to the manufacturer's protocol (Nanostring Technologies).