RNA-Seq of Homo sapiens: HUVECs in GelMA hydrogel with HIF-1 stabilization
HUVECs were seeded into a 3D GelMA hydrogel matrix and subjected to hypoxia-mimetic conditions via HIF-1 stabilization. mRNA was selectively enriched from total RNA for library preparation. The libraries were sequenced using the Illumina NovaSeq 6000 system (PE150). The study aims to delineate the transcriptomic shifts governing angiogenesis, cell migration, and vessel formation within a bioengineered bone microenvironment.