GSM9635711: KO3_2h_H3K9me1; Mus musculus; OTHER
Sorted FO B cells were stimulated with anti-IgM for 2 h, washed, bound to ConA-coated beads (Bangs Laboratories), and incubated overnight with anti-H3K36me1 (Abcam), followed by anti-rabbit IgG secondary antibodies (Antibodies Online). Samples were incubated with the pA-Tn5 adapter complex (Epicypher) for 1 h at room temperature and subjected to tagmentation at 37°C. DNA was extracted, PCR-amplified, and sequenced on an Illumina NextSeq 500 using the NextSeq 500/550 v2 kit.