GSM9778528: pancreatic CTC sample 414, GKB202-treated; Homo sapiens; RNA-Seq
Total RNA was extracted from patient-derived CTC samples using TRIzol reagent according to the manufacturer's instructions. RNA concentration and purity were assessed using a NanoDrop ND-1000 spectrophotometer. RNA integrity was assessed using an Agilent 2100 Bioanalyzer with the RNA 6000 LabChip kit. RNA-seq libraries were constructed using the Illumina TruSeq Stranded Total RNA Library Prep Gold Kit (Cat. No. 20020598) according to Illumina's official protocol. Fragment size selection was performed using AMPure XP magnetic beads.