TCR-seq of mouse tumour xenografts (LLC1 cells, injected in the flank) and treated with DMSO
Library strategy: Nested-PCR. Samples were collected from 16 mice bearing LLC1 tumours and treated with DMSO, KVS0001, DMSO + anti-PD-1, or KVS0001 + anti-PD-1 (4 mice per treatment). Total RNA was isolated using Macherey-Nagel RNA extraction kit and cDNA libraries were prepared using the SMARTer Mouse TCR a/b Profiling Kit. Bulk TCR-sequencing was performed on an Illumina MiSeq platform using the Illumina Miseq reagent kit (TCR-beta chain, paired-end reads, 2 x 250bp configuration).