GSM9289229: Induced pluripotent stem cells replicate 2; Homo sapiens; RNA-Seq
RNA was extracted using TRIzol™ Reagent according to the manufacturer's instructions Full-length cDNA was first synthesized and amplified from total RNA using the Iso-Seq Express 2.0 Kit (PacBio). The resulting full-length cDNA was then used to prepare SMRTbell libraries with the Kinnex Full-Length RNA Kit (PacBio).