GSM8753725: Male 1 Double CLD2AD SVC Fraction; Mus musculus; RNA-Seq
Stromal vascular cells from 7 mos mice were isolated by collagenase digestion .  Cell viability was measured by trypan blue exclusion to confirm a viability of greater than 97%.  Cell suspensions were loaded into a 10x Genomics Chromium Next GEM Chips and encapsulated with Single Cell 3ʹ v3 barcoded gel bead using the 10x Genomics Chromium X according to the manufacturer's instructions. Single-cell libraries were then constructed according to the 10X Genomics' instructions. Dual Index Library Kit. 3.0