Illumina NovaSeq 6000 paired end sequencing of SAMD01706041
Low-input RNA libraries were prepared using the SMART-Seq HT Plus Kit (Takara Bio; catalog R400749). Total RNA from axolotl ALM blastema was reverse transcribed with oligo-dT priming and template switching to generate full-length cDNA, followed by PCR amplification. Illumina-compatible sequencing libraries were then constructed from amplified cDNA by DNA fragmentation and adapter ligation; barcodes were added by limited-cycle PCR. Libraries were purified and size-selected (approximately 300 bp inserts). Sequencing was performed on an Illumina NovaSeq 6000 with paired-end 2x150 bp reads, targeting about 4.0 Gb per sample (~26.7 million reads; ~13.3 million read pairs). Libraries are non-stranded (unstranded).