eDNA metabarcoding marine and estuarine samples from Musquash estuary
Sea water and estuarine water samples were collected from the Musquash estuary MPA from 2022 onward. We filtered up to 1 litre of sea water per replicate (typically 3 replicates) per sample site on 0.22 micron Sterivex filters. eDNA was extracted at the Centre for Environmental Genomics Applications and sequenced using the 12S MiFishU primer set for fish, and the Leray primer set for COI for general biodiversity