RNA-Seq Analysis of C918 Human Uveal Melanoma Cell Line
Based on the poly(A) tail structure of eukaryotic mRNA, RNA-Seq libraries were constructed by enriching mRNA from total RNA using olig(dT), followed by random fragmentation with divalent cations, double-stranded cDNA synthesis using fragmented RNA as templates and random oligonucleotides as primers, end repair/dA-tailing, sequencing adapter ligation, size selection of ligated products, and PCR amplification; the library concentration and fragment size were subsequently detected to ensure quality.