GSM9703134: 6crispr_Family176_14111; Homo sapiens; RNA-Seq
Total RNA was isolated as described in the SV Total RNA Isolation System (Promega) according  to the manufacturer's instructions for “RNA Isolation from fibrous tissue”. The DNase-digestion step was expanded to 30 min at RT. 100-300 ng of RNA was used in first-strand cDNA synthesis 108 using the iScript cDNA Synthesis Kit (BioRad). RNA sequencing using poly(A) enrichment of the mRNA was performed for a total of 16 samples, comprising 3 LVNC, 5 resLVNC, 3 DCM1, and 5 resDCM1 samples.