GSM9523094: R6K, rep2; Escherichia coli K-12; RNA-Seq
Total RNA was first extracted with RNAzol RT (Sigma), and the top water phase was fed into Monarch Total RNA Miniprep Kit (NEB). The extracted RNA was further treated with DNase I. The rRNA was removed by using NEBNext rRNA Depletion Kit (Bacteria) (NEB) following the manufacturer's protocol. RNA library is prepared using Illumina Stranded mRNA Prep following the manufacturer's protocol.