GSM8595453: productive_shEP400_4_BC923; Homo sapiens; RNA-Seq
For primary CD4+ T cells: total RNA was isolated using RNeasy Micro kit which includes an on column DNase treatment. For Jurkat and J-Lat 10.6 cells: Total RNA was extracted from cells using the Qiagen RNeasy kit. RNA was DNase treated using the TURBO DNA-freeTM kit. For primary CD4+ T cells: Two days post activation of primary CD4+ T cells, non-tissue culture treated 24-well plates were coated with RetroNectin (RN) overnight at 4˚C (RetroNectin® Recombinant Human Fibronectin Fragment). RN was removed, plates washed w