GSM9157839: zs11_young_leaves_H3K9me2_rep2; Brassica napus; ChIP-Seq
Total RNA from young leaves was isolated using the RNeasy Plant Mini Kit (QIAGEN, 74904). Approximately 2 μg of RNA was used for library preparation with the Illumina TruSeq RNA kit following the manufacturer's protocol; Libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit for Illumina and sequenced on an Illumina HiSeq X-Ten system (paired-end 150-bp reads). The libraries were sequenced on an Illumina HiSeq X-Ten system.