GSM8752801: mouse, MII oocyte, single cell sample 21; Mus musculus; RNA-Seq
Full-length double-strand cDNA for each oocyte or zygote was prepared using an sc-RNA-seq libraries SMART-Seq v4 Ultra Low Input RNA Kit (Clontech, Mountain View, CA) following the vendor's instruction, which was based on the Smartseq2 protocol (Picelli et al., 2013). cDNA-seq libraries were constructed using a Nextera® XT DNA Library Preparation Kit (Illumina, Sandiago, CA, Cat. Nos. FC-131-1024, FC-131-1096) following the vendor's instruction. The libraries were quantified using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara) and sequenced on an Illumina 2500 machine with 150 bp paired end reads.