GSM8885082: PVSCs, young; Mus musculus; RNA-Seq
Young and old perivascular adipose tissues (PVAT) were dissected from aorta and cut into small pieces, which then digested with 0.2% collagenase at 37°C for 45 min. The cell suspension was filtered through 70-um Nylon cell strainer for twice to remove tissue debris. The stromal cells were resuspended in red blood cell lysis buffer and maintained in 1% bovine serum albumin (BSA)/ PBS. The scRNA-seq libraries were prepared using Chromium Single Cell 3' v2 Reagent Kit (10X Genomics) according to manufacturer's protocol.