RNA seq from ADAR fused IMP mouse liver transformants TRIBE
RNA seq from ADAR fused IMP mouse liver transformants TRIBE
Livers were snap frozen and lysed in TriReagent while shaking in the Qigen Tissuelyser with Beads. We used 1.5ml Tri-Reagent per 10mg liver. Trizol isolated RNA was DNAse digested and subsquently purified with acid phenol Libraries were created using the D-Plex RNA Kit from Diagenode with 3.5 min RNA fragmentation time and using Qiagen FastSelect Ribo blockers in the RT reaction.