GSM8620480: Gag-dNC-NC_cell_rep3; Homo sapiens; OTHER
UV-crosslinked cells and virions were lysed with RIPA buffer. Gag-RNA complexes were immunoprecipitated from lysates and RNA was subsequently extracted for CLIP-seq library construction. Libraries were constructed using sequential ligations of 3' barcoded adapter followed by 5' adapter. CLIP-seq libraries were PCR amplified using primers containing Illumina adapter sequences.