GSM9669322: AML12 EV-1; Mus musculus; miRNA-Seq
Three batches of AML12 EVs were used to isolate total RNA with QIAGEN miRNeasy kit. DNA was removed by Dnase I digestion. Approximately 200 ng of RNA was subjected to small RNA-seq. Multiplex Small RNA Library was prepared using NEBNext® Small RNA Library Prep Set according to the manufacturer's instructions