GSM9776307: ATAC_Day3_d2; Homo sapiens; ATAC-seq
Live singlet CD3+CD4+ T cells from T-allo10 cell products were purified by FACS, counted, and their cell concentration adjusted in PBS with 0.04% BSA to allow capture up to 10,000 cells per well of the Chromium Next GEM chip, which was then loaded onto a Chromium Single Cell Instrument (10x Genomics, Pleasanton, CA). RNA-seq and V(D)J libraries were prepared using the Chromium Single Cell 5′ Library, Gel Beads, & Multiplex Kit according to manufacturer's instructions.