RNA seq of HepG2 cells Ctrl vs LAP (CEBPB) OE
RNA seq of HepG2 cells Ctrl vs LAP (CEBPB) OE
Ctrl or LAP (CEBPB) overexpressed HepG2 cells RNA-seq was performed as previously described. Three biological replicates were processed per condition. FastQC was used to inspect the quality of raw sequence reads. The clean sequence reads were aligned to human genome using HISAT2. FeatureCounts was applied to quantify the number of reads mapped to each gene and then Transcripts Per Million (TPM) of each gene was calculated. Differential gene expression analysis was performed using DESeq2 package. Gene set enrichment analysis (GSEA) were conducted using clusterProfile package. Trizol reagent. RNA-seq was performed as previously described. Three biological replicates were processed per condition. FastQC was used to inspect the quality of raw sequence reads. The clean sequence reads were aligned to human genome using HISAT2. FeatureCounts was applied to quantify the number of reads mapped to each gene and then Transcripts Per Million (TPM) of each gene was calculated. Differential gene expression analysis was performed using DESeq2 package. Gene set enrichment analysis (GSEA) were conducted using clusterProfile package. FastQC was used to inspect the quality of raw sequence reads. The clean sequence reads were aligned to human genome using HISAT2. FeatureCounts was applied to quantify the number of reads mapped to each gene and then Transcripts Per Million (TPM) of each gene was calculated. 