GSM9372205: Multiome-seq, GFP+ AT2 cells,CKO mice, bleomycin lung injury, ATAC; Mus musculus; ATAC-seq
GFP+ AT2 cells were sorted by flow cytometry from bleomycin-treated(8 days post-injury)  SftpcCreERT2; Rosa26mTmG(Control) or SftpcCreERT2; Rosa26mTmG; IPF/F(CKO) mice.After sorting, nuclei were isolated from the sorted cells as follows: Cells were centrifuged at 500 × g for 5 min at 4 °C, and the supernatant was carefully removed. The cell pellets were resuspended in 1 ml chilled Lysis Buffer (10 mM Tris-HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl₂, 0.1% Nonidet P40 Substitute, 1% BSA, 0.2 U/µl RNase inhibitor) and incubated on ice for 5 min to induce cell lysis. Subsequently, the lysed cell suspension was washed three times with 1 ml chilled Wash Buffer each time to remove cytoplasmic contaminants.Nuclei permeabilization was carried out following the demonstrated protocol for single-cell multiome ATAC + Gene Expression sequencing from 10× Genomics, after which the estimated nuclei from each sample were used for the transposition step and subsequently loaded on the Chromium Next GEM Single Cell Chip J. ATAC library and gene expression library construction was performed using the Chromium Next GEM Single Cell Multiome ATAC + Gene Expression kit according to the manufacturer's instructions(CG000338 Rev A).