GSM8625216: Splenic Ly6C high macrophage, RNaseT2 deficient, replicate1; Mus musculus; RNA-Seq
Total RNA was extracted using RNeasy Mini Kits (Qiagen). 1 ng of total RNA with RIN value of more than 7.0 was used for the construction of sequencing libraries. RNA-seq libraries were prepared using an Ion AmpliSeq Transcriptome Mouse Gene Expression kit (Thermo Fisher Scientific) according to the manufacturer's instructions.