GSM8927479: PA03; Mus musculus; RNA-Seq
Total RNA extraction of samples was performed using the RNeasy (Qiagen) RNA extraction kit and according to the standard procedure provided by the manufacturer.  The resulting RNA was subjected to electrophoretic QC by Agilent 4200 TapeStation (Cat#G2991A, Agilent) and quantitative QC by Qubit 3.0 Fluorometer (Cat#Q33216, Thermo Fisher Scientific)/NanoDrop One (ND-ONE, Thermo Fisher Scientific). (Cat#Q33216, Thermo Fisher Scientific)/NanoDrop One (ND-ONE, Thermo Fisher Scientific) for quantitative QC. Qualified RNA can be used for subsequent library construction.  Translated with DeepL.com (free version) Experiments were performed using the VAHTS Stranded mRNA-seq Library Prep Kit (NR602, Vazyme) and according to the manufacturer's product instruction manual. The constructed mRNA libraries were quality checked by Qubit fluorometer (#Q33216, Thermo Fisher Scientific) and Agilent 4200 TapeStation (Cat#G2991A, Agilent). The libraries were uploaded using the illumina sequencing platform.