Total RNA-Seq of Beta vulgaris virome (Flanders_1926)
Total RNA was extracted from plant tissue with the Spectrum Plant Total RNA Kit (Sigma-Aldrich), DNase-treated, rRNA-depleted, and used to prepare strand-specific libraries with the NEBNext Ultra II Directional RNA Library Prep Kit. Libraries were sequenced paired-end (2150 bp) on an Illumina NovaSeq 6000 (GenomeScan).