GSM8819638: Control, Mycelia, CellFree; unidentified; ncRNA-Seq
C. posadasii mycelium, arthroconidia, and young (no endosporulation) spherule samples were stored in RNA Shield (Zymo Research) at −80°C until processing using the Quick-RNA™ Fungal For small RNA-seq, libraries were prepared from total RNA using the RealSeq®-Biofluids library preparation kit (RealSeq Biosciences) according to the manufacturer's instructions. Libraries for each ecotype, as well as cell-free and exosome samples, were generated separately and then pooled for the analysis. In some cases, triplicate libraries for each sample type and condition were not met, and thus, only duplicates were used in downstream analyses to avoid batch effects in the data. Finally, prepared libraries were pooled and subjected to single-end 75 cycles of sequencing using the F3 50 cycle sequencing kit (Note: reagents for 50 additional cycles above what is represented were included to account for index sequencing needs) on the Singular G4 system according to the manufacturer's instructions (Singular Genomics).