GSM9557455: Jurkat T cells, SA-CD28p, rep1; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol reagent, flash-frozen in liquid nitrogen, and subjected to sequencing by Shanghai Weihuan Biotechnology Co., Ltd. Differentially expressed genes (|log2FC| ≥ 1, p < 0.05) were identified through an interactive analysis platform. Total RNA was extracted using TRIzol reagent according to the manufacturer's protocol. RNA quality and integrity were assessed prior to library preparation. Poly(A)+ mRNA was enriched using oligo(dT) magnetic beads and fragmented into short fragments. First-strand cDNA was synthesized using random hexamer primers, followed by second-strand synthesis. The cDNA fragments were end-repaired, A-tailed, and ligated to sequencing adapters. Libraries were PCR-amplified and purified prior to sequencing. Paired-end sequencing (150 bp) was performed on an Illumina NovaSeq 6000 platform.