Nanopore sequencing of S. cerevisiae after Recombination: 5xUAS_A, 5xUAS_B, 5xUAS_D, 5xUAS_E
Genomic DNA of the recombined yeast cultures (strains Y2006, Y2008, Y2012 and Y2014)  was extracted using the YeaStar Genomic DNA kit (Zymo Research, Freiburg, Germany). The recombined promoters derived from the 5xUAS_A-B and D-E promoters were amplified using the KAPA HiFI HotStart ReadyMix PCR Kit (Roche, Basel, Schweiz) with the appropriate primer pair. The PCR products were purified using Agencourt AMPure XP beads (Beckmann Coulter) and analyzed on a 2 % agarose gel. Further, the amplicons were prepared for Nanopore sequencing according to the Ligation Sequencing DNA V14 (SQK-LSK114) protocol provided by Oxford Nanopore Technologies. The library was loaded onto Flongle flowcells (FLO-FLG114). The sequencing run lasted 24 h and generated 197 k , with N50 of 609 b. Basecalling of the Nanopore data was performed by the High-accuracy model v4.3.0, 400 bp