GSM9777098: SW48(WT), ChIP (TEAD4), rep 1; Homo sapiens; ChIP-Seq
Cells were crosslinked with 1% formaldehyde for 10 min at room temperature, and the reaction was quenched with 0.125 M glycine. DNA was purified using the ChIP DNA Clean & Concentrator kit (D5205; Zymo Research). Purified DNA was subjected to ChIP-qPCR analysis or prepared for sequencing using the NEBNext® Ultra™ II DNA Library Prep Kit (E7645S; NEB).