Illumina S
Genomic DNA libraries are prepared using the Nextera XT Library Prep Kit (Illumina, San Diego, USA) following the manufacturers protocol with the following modifications: input DNA is increased 2-fold, and PCR longation time is increased to 45 seconds. DNA quantification and library preparation are carried out on a Hamilton Microlab STAR automated liquid handling system (Hamilton Bonaduz AG, Switzerland). Libraries are sequenced on an lllumina NovaSeq 6000 (Illumina, San Diego, USA) using a 250 bp paired end protocol.