ddRAD-seq of northern gannet (Morus bassanus)
Two restriction enzymes (SbfI and MspI) were used to digest 200 ng of normalized genomic DNA. Digested samples were ligated to adaptors barcoded with four to eight base pairs (bp) for individual assignment. Uniquely barcoded samples were pooled and fragments 200 to 500 bp in length were selected using a BluePippin. Size-selected fragments were amplified via a high-fidelity PCR for 18 cycles. Libraries were sequenced in four lanes of Illumina HiSeq2500 with 100 bp, single-end sequencing.