GSM9720938: Subject TOFA_E715, 40 weeks tofacitinib; Homo sapiens; RNA-Seq
Whole blood samples were collected into PAXgene Blood RNA Tubes (PreAnalytiX/Qiagen) and RNA purified using a PAXgene Blood RNA Kit (Qiagen). RNA quality was assessed using an Agilent 2200 TapeStation and quantified by Qubit (Life Technologies). Globin RNA depletion, poly-A(+) RNA enrichment, and strand-specific library preparation were carried out using: GLOBINclear Kit, human [Invitrogen AM1980] and Fast RNA-seq Lib Prep Kit V2 (Stranded) [Abclonal RK29954]. Paired-end 150 bp sequencing was carried out on an Illumina NovaSeq X Plus instrument by Novogene USA.