RNA-Seq of Homo sapiens: human umbilical vein endothelial cells, untreated control
Human umbilical vein endothelial cells (HUVECs, denoted as HUEVC in laboratory records) were cultured under standard conditions. Total RNA was purified and libraries were constructed using PolyA-tail selection. Paired-end sequencing was conducted on an Illumina NovaSeq 6000 platform (150bp read length). This control group establishes the transcriptomic signature of quiescent endothelial cells prior to scaffold incorporation.