RNA-Seq of?Citrus paradisi: Piwsc1-infected grapefruit, 3dpi, rep1
In this study, dual RNA-seq was employed to reveal the global changes in gene expression of both?Penicillium italicum?and grapefruit during their interaction at the molecular level. The experimental design was as follows: three wounds were made on each fruit, and 10 L of spore suspension of the wild-type strain WT and the knockout strain Piwsc1 were inoculated into the wounds on the equator of different grapefruit fruits, respectively. The fruits were incubated at 26 C. At 3 days post-inoculation, mixed tissue samples (containing both?Penicillium italicum?and grapefruit) were collected from the junction of diseased and healthy tissue, immediately frozen in liquid nitrogen, and stored at -83 C. The samples were labeled as WT group and WSC group, with three biological replicates per group.