Prokaryote microbiota (16S rRNA metabarcoding) of extraction control
DNA extraction was performed using the DNeasy PowerSoil Pro Kit (Qiagen, Germany) without a sample. Illumina NovaSeq 6000 sequencing with a paired-end approach (250 bp) was conducted targeting the V3-V4 region of the prokaryote 16S rRNA gene (primer pair 341F/805R).