Tn-seq of Shigella sonnei Output 2
a transposon mutant library in S. sonnei strain 53G using transposomes prepared from mini-Tn5 and EZ-Tn5 Transposase (Epicentre).  Individual aliquots of the transposon library were thawed and added to M9 minimal media. After 18 days DNA was extracted from each LTNS culture and from a frozen aliquot of the transposon library via phenol-chloroform extraction.