Bacterial diversity of soils of the Wright Valley, Antarctica
Soil DNA was extracted using the PowerMax Soil DNA Isolation Kit and amplified with 16S rRNA gene primers 515F/806R targeting the V4 region. A single-step 30-cycle PCR was performed with HotStarTaq Plus Master Mix. Amplicons were purified, pooled, and sequenced on the Roche 454 GS FLX Titanium platform. Sequencing and pipeline analysis were conducted by the MrDNA Next Generation Sequencing Service (Shallowater, TX, USA).