GSM9100261: First replicate of HNF1B ChIP-seq from kidney; Mus musculus; ChIP-Seq
Mouse tissues were dissected and washed with ice-cold PBS and immediately transferred into liquid nitrogen and kept at -80C. For ChIP-Seq, samples were crosslinked with 1% formaldehyde for 10 minutes. After quenching, chromatin was sheared using Bioruptor. Library preparation is performed at BGI Biotech Solutions Co. Ltd (Hong Kong, China) using MGIEasy Universal DNA Library Prep Set (MGI-Shenzhen, China). Firstly, A certain amount of purified ChIPed DNA sample is taken and converted into blunt-end DNA through end repair reaction. Then an adenosine is added to the 3′ ends of the end repaired DNA through A-tailing reaction. Later, library adaptors are connected through adaptor ligation process, Finally, the library products are amplified through PCR reaction and subjected to quality control.