GSM8679687: IL-17 positive cells 13-01; Mus musculus; OTHER
TCR amplicon generation and NGS RNA was extracted from FACS-sorted  IL-17pos and IL-17neg γδ T cells from pLNs of Vγ4-/- / Vγ6-/- mice, using an RNeasy Micro Kit (Qiagen). For TRD (TRDV1 and TRDV5) repertoire analysis, the isolated mRNA was reverse-transcribed using SMARTer RACE 5'-3' PCR Kit (Clontech) with a customized protocol. Next, the CDR3 region of TRD clones was amplified via RACE PCR.