GSM9370650: Week 10- AAV2-CAG-m-IFNb-WPRE n=2; Mus musculus; RNA-Seq
Total RNA was extracted from 8 mouse RPE/choroid tissues using a RNeasy Mini Kit (Qiagen, USA, Cat. #74104) following the manufacturer's protocol.  Samples were quantified using Qubit 4.0 Fluorometer (Life Technologies, USA) and RNA integrity was checked with RNA Kit on Agilent 5300 Fragment Analyzer (Agilent Technologies, USA). Library preparation and NovaSeq Sequencing were performed at Azenta, USA. RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina following the manufacturer's instructions (NEB, USA).