Genomic insights into Enterococcus species isolated from various food sources in  Accra, Ghana.
Library preparation involves essential materials such as nucleic acids (genomic DNA or cDNA), adapters for sequencing compatibility, enzymes (DNA polymerase, restriction enzymes, T4 DNA ligase) for fragmentation and ligation, buffers and purification kits for optimal reaction conditions, and size selection tools (agarose gel electrophoresis, magnetic beads) for isolating appropriately sized fragments. Key methods include fragmentation of DNA, end repair and A-tailing for adapter preparation, ligation of adapters to DNA fragments, and size selection to purify and select the desired fragment sizes.