GSM9202410: cell, ZA, rep3; Mus musculus; RNA-Seq
Total RNA of BMDMs treated with or without ZA was extracted using Trizol reagent (ThermoFisher, 15596018CN) following the manufacturer's procedure. RNA purity and quantification were evaluated using the NanoDrop 2000 spectrophotometer (Thermo Scientific). RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies). Then the libraries were constructed using VAHTS Universal V10 RNA-seq Library Prep Kit (Premixed Version) according to the manufacturer's instructions. The libraries were then sequenced on an Illumina Novaseq 6000 platform and 150 bp paired-end reads were generated. The transcriptome sequencing and analysis were conducted by OE Biotech. RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocals.