RNA-seq of Limonium bicolor: EV-1
Total RNA was extracted from leaves at SIX-LEAF stage as three biological replicates. RNA concentration and quality were measured by Nanodrop and then by Qubit, before checking RNA integrity on an Agilent 2100 bioanalyzer. cDNA libraries were prepared from polyA-selected mRNA samples with Oligo(dT) magnetic beads. Library concentrations were determined on a Qubit 2.0, followed by Agilent 2100 bioanalyzer to estimate insert size, and the final library size was about 350bp. Library titer was precisely determined by qPCR (QuantStudio 3, ABI, USA) according to the instruction of KAPA LIBRARY QUANTIFICATION KITS. After quantification, the libraries were pooled and sequenced as 150-bp paired-end reads on an Illumina Novaseq platform.