HiC of lutjanus malabaricus: male
In situ Hi-C libraries were constructed from crosslinked tissue/cells (1% formaldehyde, 10 min), digested with MboI restriction enzyme overnight at 37C, end-filled with biotin-14-dCTP, and proximity-ligated with T4 DNA ligase at 16C for 4 h. Crosslinks were reversed with proteinase K at 65C overnight. Purified DNA was sheared to ~400 bp, biotin-enriched using streptavidin beads (Dynabeads MyOne C1), and libraries prepared with the NEBNext Ultra II DNA Library Prep Kit. Paired-end 150 bp sequencing on Illumina HiSeq X Ten. Two biological replicates per sample.