GSM9558799: 6_6_3_S8; Mus musculus; RNA-Seq
RNA was extracted using QIAGEN RNeasy mini kit Read adapters were detected using BBMerge (v37.02) [1] and subsequently removed with cutadapt (v1.8.1) [2]. Processed raw reads were then aligned to mouse genome mm10 using STAR (v2.7.7a)[3]. Gene expression was evaluated as read count at gene level with RSEM (v1.3.0) [4] and Gencode gene model vM21. Gene express data were then normalized and differential expression between experimental groups were evaluated using DEseq2 [5].