GSM9523812: SC5314 wor2△/△, bio rep1; Candida albicans; RNA-Seq
RNA extraction was performed by disrupting cells with Zirconia beads and isolating RNA using 25:24:1 phenol:chloroform:isoamylalcohol in combination with a Qiagen Rneasy Mini Kit Sequencing libraries were generated by purifying messenger RNA from total RNA using poly-T oligo–attached magnetic beads. The first cDNA was synthesized using random hexamer primers, followed by sequencing of both ends of cDNA fragments using the Illumina platform