GSM8713139: WM, CD8, 4; Homo sapiens; RNA-Seq
Primary cells were isolated, with an additional step of incubating homogenized samples before enzymatic digestion with the transcriptional inhibitor triptolide (10 μM), anisomycin (27.1 μg/ml), and actinomycin D (5 μg/ml). Sorted cells were lysed in 350 ml buffer RLT plus 1% β-mercaptoethanol and stored at -80°C until further processing. Total RNA was isolated with the RNeasy MicroKit according to the manufacturers' instructions, and RNA sequencing was performed (GenomeScan). NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina (E6420L)