Nascent transcriptome profile of silenced MCF-7 using ASO mediated knock-down for PVT1
Nascent transcriptome profile of silenced MCF-7 using ASO mediated knock-down for PVT1
Nascent RNAs were performed as proposed by Khodor et al 2011 RNAs were extracted using TRIzol (Life Technologies, Thermo Fisher), according to the manufacturerâ€™s instructions.  lllumina Stranded Total RNA prep Ligation with Ribo-Zero Plus kit (Cat. 20040529 Illumina)