GSM9364294: Mouse Spleen, Hv1 knockout, Sham, 6 weeks after SCI, Replicate 2; Mus musculus; RNA-Seq
Following euthanasia 7 days and 6 weeks after SCI, mice were perfused with 50 mL of ice-cold normal saline. Total RNA was extracted from the spinal cord, lung, and spleen tissue with the miRNeasy Mini Kit (Qiagen, Cat# 74104).  In brief, approximately 600ul of QIAzol were added to each sample and disrupted in a Precellys 24 Touch homogenizer. The aqueous phase containing RNA was collected, mixed with ethanol, and applied to a silica membrane spin column. After a series of washes to remove contaminants, RNA was eluted in RNase-free water. A minimum of 100ng was sent to Novogene Bioinformatics Technology Co., Ltd. (Sacramento, CA) for downstream sequencing assays. Library preparations were carried out by Novogene (Sacramento, CA) for sequencing on the Illumina platform (paired-end, 150 bp reads). mRNA was enriched through poly-A pulldown.