GSM8595831: D21.A_S9; Homo sapiens; ssRNA-seq
Prior to RNA extraction, media was removed and bioprinted liver tissues were frozen in liquid nitrogen and stored at −80 °C until ready for RNA extraction. Tissues were homogenized using a homogenizer, and total RNA was isolated from homogenized tissue with RNeasy Mini Kit (QIAGEN, Valencia, CA). Strand-specific mRNA-sequencing libraries (polyA+) were generated and barcoded using Illumina's TruSeq stranded mRNA library prep kits. Each RNA-Seq library was sequenced on an Illumina HISEQ.