GSM9423183: Control_M_Input; Ctenopharyngodon idella; ChIP-Seq
1× 107 cells were fixed with 1% formaldehyde (Sigma-Aldrich, cat. no. F8775) for 10 min at room temperature, followed by quenching with 125 mM glycine for 5 min. After lysis, cell nuclei were pelleted by centrifugation at 1000 rpm for 5 min at 4°. The chromatin was fragmented through sonication to achieve an average DNA fragment size of 200–500 base pairs. ChIP DNA libraries were prepared using the NEBNext® Ultra™ DNA Library Prep Kit for Illumina (NEB). End repair/A-tailing, adaptor ligation, and USER enzyme treatment were performed according to the manufacturer's instructions. Libraries were amplified by 8–10 PCR cycles, size-selected, and purified using AMPure XP beads. Library quality was assessed by Qubit and high-sensitivity DNA chip analysis.