GSM8787840: Multiomics, Control, GEX; Mus musculus; RNA-Seq
Wound bed tissue was excised, minced, and digested with a collagenase-containing enzyme mix to obtain a single-cell suspension. After filtration through a 70 µm cell strainer to remove debris, cells were washed. For  permeabilization, cells were treated with DIG lysis buffer (20 mM Tris-HCl pH 7.4, 150 mM NaCl, 3 mM MgCl2, 0.01% Digitonin and 2 U µl−1 of RNase inhibitor) for 5 min on ice, followed by adding 1 ml of chilled DIG wash buffer (20 mM Tris-HCl pH 7.4, 150 mM NaCl, 3 mM MgCl2 and 1 U µl−1 of RNAse inhibitor) and inversion before centrifugation at 500g for 5 min at 4 °C. The supernatant was discarded, and cells were resuspended in DIG wash buffer, followed by counting using trypan blue and a Countess II FL Automated Cell Counter. Cells were processed according to the Chromium Next GEM Single Cell Multiome ATAC + Gene Expression user guide。 Multi-omics library constuction protocol described in Xu. et al.