GSM8819384: Mouse cecal contents, Gastric administration of edible alcohol, Bacteria; mouse metagenome; OTHER
Total genomic DNA was extracted from *** samples using the TGuide S96 Magnetic Soil /Stool DNA Kit (Tiangen Biotech (Beijing) Co., Ltd.) according to manufacturer's instructions. The hypervariable region V3-V4 of the bacterial 16S rRNA gene were amplified with primer pairs 338F: 5'- ACTCCTACGGGAGGCAGCA-3' and 806R: 5'- GGACTACHVGGGTWTCTAAT-3'.PCR products were checked on agarose gel and purified through the Omega DNA purification kit (Omega Inc., Norcross, GA, USA). The purified PCR products were collected and the paired ends (2 × 250 bp) was performed on the Illumina Novaseq 6000 platform. (Please adjust the primer according to the actual amplification sub region) Total genomic DNA was extracted from *** samples using the TGuide S96 Magnetic Soil /Stool DNA Kit (Tiangen Biotech (Beijing) Co., Ltd.) according to manufacturer's instructions. The hypervariable region V3-V4 of the bacterial 16S rRNA gene were amplified with primer pairs 338F: 5'- ACTCCTACGGGAGGCAGCA-3' and 806R: 5'- GGACTACHVGGGTWTCTAAT-3'.PCR products were checked on agarose gel and purified through the Omega DNA purification kit (Omega Inc., Norcross, GA, USA). The purified PCR products were collected and the paired ends (2 × 250 bp) was performed on the Illumina Novaseq 6000 platform. (Please adjust the primer according to the actual amplification sub region)