Using Multiple Metrics to Analyze Bloom Dynamics of Tripos muelleri in the Gulf of Maine during Summer 2023
Primary PCR using primers designed for the V9 region included the forward primer (5-ACACTGACGACATGGTTCTACAGT-ACACACCGCCCGTC-3) and the reverse primer (5-TACGGTAGCAGAGACTTGGTCTTGATCCTT-CTGCAGGTTCACCTAC-3) (Stoeck et al. 2010), with Fluidigm common oligonucleotides (CS1-TS-F, CS2-T2-R) fused to the 5 ends.