GSM9422430: RNA-seq, leaf, J2_206, chemical fertilizer, Year 2022; Oryza sativa; RNA-Seq
The most expanded leaf was collected from 9:00 to 11:00 am as one biological replicate, immediately frozen in liquid N, and stored at -80 °C until sample crushing. Frozen samples were crushed by multi-specimen cell disruption device (BMS-A20TP, BMS) just before RNA extraction. Total RNAs from each sample were extracted using RNeasy Plant Mini Kit (QIAGEN), referring to the manufacture's protocol. One biological replicate was obtained for each JAM2 line at 6 weeks after transplanting:  July 1, 2022 and June 29, 2023. RNA-sequencing (RNA-seq) libraries were prepared using the NEBNext Ultra II Directional mRNA-seq kit for Illumina (New England Biolabs), following the manufacturer's protocols.