GSM8917387: PA-1 knockout replicate 3, RNA-Seq; Homo sapiens; RNA-Seq
Total RNA from  DGCR8 knockout PA-1 cells was extracted from a 60% confluent T75 cell flask  with the miRNeasy Mini Kit (Qiagen), following the protocol for Purification of Total RNA, Including Small RNAs, from Animal Cells was followed, including the additional on-column DNAse digestion. For total RNA-seq, after extraction, ≥ 200 ng of total RNA was used for rRNA removal stranded library sequencing with DNBseq PE100 by BGI Genomics.