GSM9251656: C57BL/6J mice injected with LPS 72hr_2; Mus musculus; RNA-Seq
Wild-type C57BL/6J mice were intraperitoneally injected with 5mg LPS (L2880; Sigma-Aldrich) with or without 100mg IL-10R blocking antibody (112702; BioLegend), or phosphate buffered saline (DPBS; 14200075; ThermoFisher Scientific) for 72 or 24 hours prior to BM harvest for cell sorting using BD FACSAria Fusion sorter at the UCI Institute of Immunology Flow Cytometry Facility. Cells were sorted, using the HSPC staining protocol aforementioned, into RNA lysis buffer (R1060; Zymo-Research) and immediately processed using Zymo-Research Quick-RNA MiniPrep kit for RNA isolation as per manufacture's protocol (R1058; Zymo-Research). Samples were stored at -20°C until sequencing at the UCI Genomics Research and Technology HUB.