Hi-C data used for genome scaffolding.
The library construction involved the following steps: crosslinking cells with formaldehyde, digesting DNA with a suitable 4-cutter restriction enzyme (Dpn II), filling ends and labeling with biotin, ligating the resulting blunt-end fragments, and purifying and randomly shearing DNA into 300500 bp fragments. Following quality control assessments using the Qubit 2.0, an Agilent 2100 instrument (Agilent Technologies, CA, USA), and quantitative PCR, 150 bp paired-end sequencing of the Hi-C library was conducted on the Illumina Novaseq 6000 platform.