DNBSeq of Microsporidia MB positive Anopheles gambiae s.s.: whole individual mosquito
Quality checks of high molecular weight extracted gDNA was done using Qubit Fluorometer and NanoDrop. Sample integrity is done using agarose gel electrophoresis.DNA fragmentation was done with Covaris and detected by Agilent 2100 followed by end-repair, dA tailing, and magnetic bead purification. Library preparation was conducted using KAPA HiFi HotStart Amp Kit, QC, and circularization. The library was amplified to make DNA nanoballs (DNB) used in the selected DNBSeq sequencing technology. Short, paired-end read sequencing (2150bp reads) is done using the DNBSEQ platform.