GSM8819690: Hepa1-6; Mus musculus; RNA-Seq
Subcutaneous xenografts derived from Hepa1-6 and B16 cells were generated in immunocompetent C57BL/6J mice. Subcutaneous xenografts were dissociated and the single-cell suspensions were obtained using the MACS Miltenyi Mouse Tumor Dissociation kit (130-096-730, Miltenyi Biotec, Germany) on gentleMACs dissociator with Octo Heaters (130-096-427, Miltenyi Biotec, Germany) according to manufacturer's instructions. Cell suspensions were passed through a 70-µm filter twice. CD45+ immune cells were then sorted from the cell suspension using flow cytometry (Cat# 35-0451-U100, TONBO Biosciences, USA), and then subjected to the BD Rhapsody Single-Cell Analysis System (BD Biosciences, USA) following the manufacturer's guidelines. The scRNA-seq libraries were constructed using BD Rhapsody WTA Amplification Kit (BD Biosciences, USA). WTA libraries and LMO libraries were pooled and sequenced on NovaSeq 6000 (Illumina, USA), and the data was processed using the BD Rhapsody WTA analysis pipeline (v1.9.1; BD Biosciences, USA). WTA reads were aligned to the mm10 reference mouse genome, allowing a maximum of 1 mismatch.