GSM8751775: Myoblast CM1 Treated (replicate 2); Homo sapiens; OTHER
WGBS was performed on Myoblast CM3 using standard protocols {Chen, 2016 #4481}. Additionally, methylation profiling of CM3, CM1, and CM6 was conducted using enzymatic methyl-seq (EM-seq) {Vaisvila, 2021 #4473}. This process involved enzymatic oxidation of 5mC and 5hmC (by TET2), providing protection from further deamination by APOBEC3A while unmethylated cytosine are deaminated to uracil. Briefly, 0.2 µg of DNA was used to prepare EM-seq libraries in duplicate. The libraries were then cleaned using NEBNext® sample selection beads, pooled, and diluted to 1.5 nM for sequencing on a NovaSeq (Illumina) Six EM-seq libraries are made for three myoblast strains i.e., CM1, CM3 and CM6, two replicates each. Similarly, myoblast stain CM3 were used to make two libraries. EM-seq, WGBS