GSM9131107: MEFs, NIL, rep B; Mus musculus; RNA-Seq
At 4 days post infection, conditions expressing SNAP-p53DD were stained using a fluorescent SNAP substrate (New England Biolabs, #S9105S), then all conditions were dissociated with trypsin. Cells were resuspended in N3 media and kept on ice for fluorescence-activated cell sorting (FACS). Cells were sorted on live, single-cells for conditions without SNAP, and SNAP positive for conditions with SNAP-p53DD. Total RNA was extracted from 50k sorted cells using the Monarch® Total RNA Purification Kit (New England Biolabs, #T2010S). ERCC RNA spike-in controls (Thermo Fisher Scientific, #4456740) were added to the lysis buffer prior to RNA isolation. Libraries for RNA-seq were prepared using the NEBNext Ultra II Directional RNA Prep Kit.