RNA-seq of Brachyelytrum erectum: Laura Hare Nature Preserve population
Total RNA was extracted from young shoots of Brachyelytrum erectum using the Qiagen RNeasy Plant Mini Kit. The RNA samples were treated with the Invitrogen DNA-free DNA Removal Kit  to remove contaminatiing DNA. High-quality RNA was then submitted to sequencing facility (Novogene Inc.) for transcriptome sequencing.  Directional mRNA sequencing, following poly(A) selection, was performed on either the Illumina NovaSeq 6000 or NovaSeq X Plus platform to obtain paired-end reads of 150 bp.