Multiplexed Assay of Variant Effect for human G6PD activity in Saccharomyces cerevisiae
Plasmid DNA was extracted using ZymoPURE II Plasmid Midiprep Kit, linearized by restriction digest with SphI-HF, prepared using SMRTbell Express Template Prep Kit 2.0 according to manufacturers instructions, followed by nuclease clean-up and an additional cleaning with 0.45 ratio of AMPure PB beads