GSM9670582: Villarino-18829-001_GEX3; Mus musculus; RNA-Seq
Total RNA isolated from TRIzol by phenol/chloroform extraction, then mRNA enriched with NEBNext Poly(A) mRNA Magnetic Isolation Module For bulk RNA-seq, sequencing libraries were generated with NEBNext Ultra II Library Prep Kit for Illumina (E7645L, New England Biolabs). For scRNA-seq , sequencing libraries were generated with 10X Chromium Single Cell 3' V3 chemistry kit.