Cowpea (UCR369) - MS C Rep 2
Cowpea leaf RNA from two genotypes under control and waterlogging at vegetative, flowering, and maturity stages was isolated and DNase-treated. mRNA was captured with poly-T oligo-dT beads. Stranded Illumina libraries (insert 250350 bp) were prepared (fragmentation, cDNA synthesis, end repair, adapter ligation, PCR), uniquely indexed, and sequenced as PE150 on an Illumina NovaSeq 6000.