GSM8678004: LMMP 6-month female 3; Mus musculus; RNA-Seq
Frozen tissue samples each in 1.5mL tubes were submerged in 375 uL of RLT buffer with β-mercaptoethanol. Tissue and buffer were transferred to a ice-cold 2 mL Qiagen sample tube with a 5mm stainless steel bead and  lysed in a TissueLyzer at full speed for 3 minutes. RNA isolation was completed with the Qiagen RNeasy Mini, Animal Tissue and Cells with DNA Digest protocol. Standard Illumina stranded mRNA prep ligation kit was used with 100ng total RNA input per sample. 2x50 bp paired-end reads were sequenced.