GSM9521108: high-fat diet3; Mus musculus; RNA-Seq
RNA was harvested using Rneasy mini plus kit (Qiagen). 1μg of total RNA was used for the construction of sequencing libraries. mRNA sequencing libraries were constructed using the Hieff NGS Ultima Dual-mode mRNA Library Prep Kit for Illumina (Yeasen Biotechnology, Shanghai, China) in accordance with the manufacturer's protocol.