GSM8711998: Wild-type U87 cells, replicate 1; Homo sapiens; RNA-Seq
Total RNA was isolated from cells using the Magzol Reagent (Magen, China)according to the manufacturer's protocol. The quantity and integrity of RNA yield was assessed by using the K5500(BeijingKaiao, China) and the Agilent 2200 TapeStation (Agilent Technologies, USA) separately. Briefly, rRNAs were removed from Total RNA usingQIAseqFastSelct-rRNA HRM KIT(QIAGEN,Germany)and fragmented to approximately 200bp. Subsequently, the purified RNAfragments were subjected to first strand and second strand cDNA synthesisfollowing by adaptor ligation and enrichment with a low-cycle according to instructions of NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina(NEB, USA). The purified library products were evaluatedusing the Agilent 2200 TapeStation and Qubit(Thermo Fisher Scientific, USA). The libraries were sequencedby Illumina (Illumina, USA) with paired-end 150bp at Ribobio Co. Ltd (Ribobio, China).