Transcriptome profile of silenced MCF-7 using ASO mediated knock-down for three lncRNAs
Transcriptome profile of silenced MCF-7 using ASO mediated knock-down for three lncRNAs
Cells were washed twice with cold PBS, harvested by scraping and collected in a tube. RNAs were extracted using TRIzolTM (Life Technologies, Thermo Fisher), according to the manufacturerâ€™s instructions. Libraries preparation for transcriptome profiling was performed by using the TruSeq Stranded Total RNA Library Prep Gold (Cat. 20020599, Illumina, San Diego, California, USA)