GSM8712339: CD8, Spleen CD8 T cell enriched, FB; Mus musculus; OTHER
Immune cells were isolated from splenocytes using magnetic enrichment or orthotopic KPC2a PDA tumors using collagenase digestion and percoll enrichment. Samples were uniquely barcoded with oligo tagged antibodies prior to capture and single cell RNA sequencing. Cells were captured and the library was constructed with the 10X Genomics Chromium Next GEM Single Cell 5' Reagent Kits v2 (Dual Index) according to the manufacturer's instructions. scRNAseq. Libraries were generated for feature barcoding, TCR, and gene expression.