Groundwater metagenome: 16S rRNA amplicon sequencing of site C1-C-Napon
16S rRNA gene amplicon libraries were constructed targeting the V3-V4 region using primers 341F (5-CCTACGGGNGGCWGCAG-3) and 805R (5-GACTACHVGGGTATCTAATCC-3). DNA was extracted from 0.45 m membrane filters using the DNeasy PowerWater DNA Isolation Kit (Qiagen). Library quality was verified via Picogreen (Victor 3 fluorometry), Agilent 2000 Bioanalyzer (DNA 1000 chip), and qPCR according to the Illumina qPCR Quantification Protocol Guide. Sequencing was performed on the Illumina MiSeq platform (2x300bp).