GSM8679399: VICs, Control siRNA, Osteogenesis medium-1; Homo sapiens; RNA-Seq
Control VICs and CALD1-knockdown VICs cultured in Osteogenesis Differentiation Medium or MesenPRO RS™ Medium for 4 w were collected using 700 µL of QIAzol Lysis Reagent (QIAGEN). Total RNA was purified using the miRNeasy Micro Kit (QIAGEN) according to the manufacturer's instructions. for library preparation, the KAPA mRNA Capture Kit (KAPA, Catalog No. KK8440) and MGIEasy Fast RNA Library Prep Set (MGI, Catalog No. 940-000889-00) were used. The constructed RNA libraries were sequenced on a DNBSEQ-T7RS using the DNBSEQ-T7RS High-throughput Sequencing Set.