GSM8713120: Sample 1, 0 hour, replicate 2; Mus musculus; RNA-Seq
Eight-week-old male C57BL/6J mice  were anesthetized with 500 mg/kg tribromoethanol  and were killed by cervical dislocation. After the animals were sacrificed, brain tissues (hippocampus) were isolated, placed in 2 mL enzyme-free centrifuge tubes, and stored at 25 ̊C at constant temperature. Postmortem tissues were sampled at 0 h (snap frozen, H0), 24 h (H24), 36 h (H36), 48 h (H48), and 54 h (H54), followed by snap frozen in liquid nitrogen and stored at -80 ̊C until use. For the reliability of the data, we maintained the consistency of the environment for each sample and set up biological replicates of three mice per group. Sorted nuclei were processed using the 10× Chromium Single Cell 3′ Library and Gel Bead Kit v3, nuclei were immediately loaded onto a Chromium Single Cell Processor for barcoding of RNA from single nuclei. The quality of cDNA was assessed using the Agilent× v3.1 to generate the cDNA libraries. The quality of cDNA was assessed using the Agilent 2100 Bioanalyzer System. Sequencing libraries were constructed according to the manufacturer's instructions and then sequenced in a NovaSeq 6000 sequencing system.