16S rRNA sequencing of Vespa velutina sample A108
The sequencing library was prepared from total genomic DNA extracted from the gut content of hornet samples using a commercial DNA extraction kit. The bacterial 16S rRNA V4 region was amplified using specific primers and PCR conditions following standard amplicon sequencing protocols. PCR products were purified, quantified, pooled, and used to construct the sequencing library according to the manufacturer's instructions. The final libraries were sequenced on the DNBSEQ-G99 platform using paired-end 150 bp mode.