RNA-Seq of Human BCR
Sequencing libraries were constructed using the Nextera XT DNA Library Prep Kit (Illumina), followed by PCR amplification, purification with Agencourt AMPure XP beads, quantification with the Qubit DNA High Sensitivity kit, size inference with the High Sensitivity DNA Kit on the Bioanalyzer 2100, and sequencing on the Illumina MiSeq System using the Miseq Reagent Kit v3, pooling 34 different libraries equimolarly to 18 picomolar.