GSM9557544: Fungal hyphae cells, D-3; Orbilia oligospora; RNA-Seq
Total RNA was extracted from tissue samples, the concentration and purity of the extracted RNA were detected by Nanodrop2000, RNA integrity was detected by agarose gel electrophoresis, and RIN value was determined by Agilent2100. A single library construction requires that the total amount of RNA is ≥1ug, the concentration is ≥35ng/μL, OD260/280 is 1.8 - 2.2, and OD260/230≥1.0. The libraries were prepared for sequencing using standard Illumina protocols.