GSM8753892: starvation response, colonies put from YMG to water agar; 24h; control is DM60h, replica 2; Coprinopsis cinerea AmutBmut pab1-1; RNA-Seq
RNA was extracted using Quick-RNA Miniprep kit (Zymo Research, USA, catalog number: R1054), following the manufacturer's instructions. The libraries were generated using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen, Vienna). To begin, 100 ng of total RNA was used as input for first strand cDNA generation with an oligo-dT primer, followed by RNA removal. Next, second strand synthesis was initiated with random priming and the resulting products were purified with magnetic beads. Finally, the libraries were amplified and barcoded using PCR. The Agilent 4200 TapeStation (Agilent) was used to assess all libraries for the formation of adapter dimers during PCR. The QuantSeq libraries were sequenced on the Illumina NextSeq550 platform, producing 75 bp single-end reads.