RNA-Seq of Homo sapiens: primary bone marrow mesenchymal stem cells, untreated control
Total RNA was extracted from primary human bone marrow mesenchymal stem cells (BMSCs) using TRIzol reagent. Libraries were prepared using the Illumina Stranded mRNA Prep protocol with PolyA selection to enrich for coding transcripts. The finalized libraries were sequenced on the Illumina NovaSeq 6000 platform using a 2 x 150bp paired-end configuration. This dataset provides a baseline transcriptomic profile for cells cultured in 2D standard conditions.