B6 desert steppe soil 16S rRNA V3-V4 amplicon, June, 0-5 cm, replicate 2
The V3-V4 region of the bacterial 16S rRNA gene was amplified from desert steppe soil DNA using primers 341F and 806R. PCR products from three technical replicates per sample were pooled, purified, quantified, and sequenced on an Illumina platform using paired-end 2 x 300 bp sequencing.