GSM9047765: hPSC, DMSO, untreated, rep3; Homo sapiens; RNA-Seq
Qiagen Rneasy kit For paired end RNA-seq, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library then underwent end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and RT-PCR for quantification and bioanalyzer for size distribution. Quantified libraries were pooled and sequenced on an Illumina NovoSeq X plus.