miRNA-seq of Mus musculus: E18.5 pooled cerebellum
E18.5 whole cerebella were dissected and pooled. RNAs were extracted from the pooled cerebella with the mirVana miRNA Isolation Kit into two fractions: >200 nt and < 200 nt. Small RNAs 20-30 nt in length were purified from the < 200 nt fraction. Reverse transcription followed using Maxima H minus RT (200 U/). The cDNA was purified using PCR Clean DX bead cleanup. The paired-end sequencing library was prepared following the BC Cancer Genome Sciences Centre strand-specific, plate-based library construction protocol on a Microlab NIMBUS robot.