GSM8712691: G73_20_ctrl; Drosophila melanogaster; ncRNA-Seq
Small RNAs from ovaries collected at permissive (25°C) and non-permissive (20°C) temperature, for Piwi-sKD, were isolated using TraPR ion exchange spin columns (Lexogen, Catalog Nr.128.08). The libraries were performed by MGX-Biocampus Montpellier platform using the NEBNext® Small RNA Library Prep Set for Illumina® from NEB. 150 nt paired-end read (28 bp for R1 and 90 bp for R2) sequencing on NOVASEQ 6000 apparatus was performed by MGX. Only the R2 file contains information.