PacBio HiFi of mexican lime leaf tissue
HMW DNA was extracted from young leaves and shoot apices from a single thornless mexican lime tree (BioSample SAMN42593883) using an in-house CTAB protocol at Brigham Young University DNA Sequencing Center (Provo, UT). The PacBio SMRTbell library (~20kb) was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol. The library was loaded onto a 8M SMRT cell and run on a PacBio Revio for 24 hours.