Simplified sequencing of Hypophthalmichthys molitrix (a primary freshwater aquaculture species in China)
1. DNA extraction. The DNA of the sample was extracted using phenol/chloroform extraction method.2. Restrictive endonucleases digest DNA. Select BsaXI enzyme as the restriction endonuclease for genomic DNA.3. Joint connection. Connect Solexa specific ligands (slx-ad1 and slx-ad2) to the enzyme cleavage products mentioned above.4. PCR amplification and recovery of target fragments.5. Library quality control.