GSM9635824: Resident airway macrophages, engulfing, rep3; Mus musculus; RNA-Seq
RNA was isolated using the RNeasy Plus Micro isolation kit (QIAGEN) per the manufacturer's instructions with addition of β-mercaptoethanol to RLT Plus buffer. RT-PCR was performed using the SensiFast Hi-ROX One-Step Kit (Meridian Bioscience) per the manufacturer's instructions. Libraries were built using the standard Kapa Biosystems library preperation kit.