GSM9230199: H11, replicate 1, scRNAseq; Gallus gallus; RNA-Seq
Cell samples for scRNA-seq were dissociated using the Multi Tissue Dissociation Kit 3 (Miltenyi Biotec, 130-110-204). Briefly, the dissected cranial tissues were rinsed with PBS, followed by the addition of the enzyme mix provided in the kit. Samples were incubated at 37°C for 10 minutes, then gently triturated by pipetting. This was followed by a second 10-minute incubation at 37°C and additional pipetting to obtain a single-cell suspension. The resulting suspensions were washed twice with cold PBS and filtered through a 70 μm MACS SmartStrainer (Miltenyi Biotec) to remove cell clumps. Library preparation was performed according to the manufacturer's instructions for the 10× Chromium single-cell kit (10x Genomics). The libraries were then pooled and sequenced on two lanes of  NovaSeq 6000 sequencer (Illumina).