GSM9701377: Clone B2, 24 hpi, sample 1; Mus musculus; RNA-Seq
The liver tissue was homogenised in 500 µL of TRIzol reagent (Thermo Fisher Scientific), to which an additional 500 µL of TRIzol was added. RNA was then isolated using the mirVana™ miRNA Isolation Kit (Ambion), following the manufacturer's protocol. RNA concentration and integrity were assessed spectrophotometrically using a NanoDrop 2000 (Thermo Fisher Scientific) and with an Agilent 2100 Bioanalyzer employing the RNA 6000 Pico Assay kit (Agilent Technologies). Potential genomic DNA contamination was eliminated using the TURBO DNA-free Kit (Thermo Fisher Scientific, Hampton, NH, USA). Library preparation and high-throughput RNA sequencing were conducted by BGI (Shenzhen, China) using the Illumina HiSeq 4000 platform