Sequencing of Staphylococcus strains from sheep
Staphylococcus isolates were obtained from the samples after culture in order to obtain pure staphylococcal colonies. Total genomic DNA was extracted from a bacterial inoculum 0.5 Mc Farland after treatment with proteinase K, followed by lysis combined with bead beating instrument (Zymo) and automated magLEAD 12Gc system (PSS, BioServices, Stockholm). Library preparation was performed using the MGIEasy Fast FS Library Prep Set and the MGIEasy Dual Barcode Circulization Kit (MGI). Paired-end sequencing (150 bp) was carried out on the DNBSEQ-G99 platform with the High-throughput Sequencing Set (G99 SM FCL PE150). As the DNBSEQ-G99 is not listed in the SRA database, DNBSEQ-G400 was selected as the most appropriate equivalent.