GSM9524040: mice infected with PBS-3; Mus musculus; RNA-Seq
At 24 hours post-infection, lungs were harvested, minced, and subjected to dissociation into single-cell suspensions using a gentle tissue dissociation kit (Miltenyi Biotec) according to the manufacturer's instructions. Cell viability was assessed by trypan blue exclusion and exceeded 85% in all samples. Single-cell libraries were constructed using the Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (10x Genomics) and sequenced on an Illumina NovaSeq 6000 platform by Beijing Novogene Co., Ltd. Raw sequencing data were processed with Cell Ranger (v7.1.0) using the mouse reference genome (mm10) for alignment and gene counting.