Metagenome sequencing of anammox bacteria: biomass from a lab-scale reactor under low  aeration condition
Genomic DNA was extracted from anaerobic ammonium-oxidizing granular sludge using the DNeasy PowerSoil Pro Kit. Libraries were prepared with the Illumina DNA Prep Kit following the standard protocol, including fragmentation, adapter ligation, and PCR amplification. Library quality was assessed using a Qubit fluorometer and an Agilent 4150 TapeStation. Pooled libraries were sequenced on an Illumina MiSeq to generate 2150 bp paired-end reads.