GSM8619347: VC_snRNAseq-12_Animal2_Central_Nucleus; Macaca mulatta; RNA-Seq
For human samples, the amygdala was dissected from fresh frozen postmortem human tissue and 100 micron coronal sections of amygdala from fresh frozen postmortem human tissue were obtained by cryosectioning. For nonhuman primates, coronal brain blocks were flash-frozen immediately prior to tissue punches localized to specific amygdala subregions. All nuclei were isolated and barcoded with 10x Genomics Chromium. Libraries were prepared using 10x Chromium Next GEM Single Cell 3' v3.1 (dual-index) kit according to manufacturer's instructions. single-nucleus RNA-sequencing