GSM9291871: MEF_untreated_rep3; Mus musculus; ATAC-seq
After the appropriate treatment, cells were trypsinized and collected in media. Samples were then processed and tagmented using TD buffer and TDE1 enzyme (Illumina). EdU-positive samples were biotinylated via Click-IT (Invitrogen) and purified with Ampure XP beads (Beckman Coulter). Biotinylated products were captured using MyOne Streptavidin T1 beads (Thermo Fisher Scientific), and the unbound fraction (unreplicated/ bulk) in the supernatant from streptavidin pulldown was purified using the MinElute Reaction Cleanup kit (Qiagen). Samples were amplified using NEBNext High-Fidelity 2x PCR Master Mix (NEB) and final libraries were purified using Ampure XP beads.