PacBio HiFi of homo sapiens: BJ
2-5 ug of HMW gDNA was used as input for SMRTbell Prep Kit V3.0 (PacBio, Cat. No. 102-182-700). Mechanical shearing of gDNA was performed with Megaruptor 3 (Diagenode, Cat. No. B06010003) using the standard protocol with target size of 15-20 kb. Fragmentation was analyzed by Femto Pulse System (Agilent, Cat. No. M5330AA). SMRTbell libraries were sequenced on the PacBio Revio platform with SMRT Cell tray (PacBio, Cat. No. 102-202-200), loaded at 240-260 pM, with 30-hour movie collection times.