CC3 (CRX2020075; CRA030417)
Genomic DNA was extracted from each fecal sample utilizing the FastPure Feces DNA Isolation Kit (Vazyme Biotech Co., Ltd., Nanjing, China) according to the manufacturer's protocol. Approximately 1 microg of extracted genomic DNA was subjected to random fragmentation into segments of approximately 350 bp using a Covaris ultrasonic disruptor for library construction.