GSM9157731: V290A NSP14 SARS-COV-2 Passage1; Chlorocebus sabaeus; RNA-Seq
RNA was further purifified using the RNA simple Total RNA Extraction Kit (Tiangen, DP419). cDNA was prepared using a transcription kit (TransGen Biotech). For PCR amplifification of the NSP14 coding region the following primers were used: NSP14-forward (5'-GAAAATGTAACAGGACTCTTTAAAGATTGTAGTAAGG-3') and NSP14-reverse (5'-CTGAAGTCTTGTAAAAGTGTTCCAGAGG-3').