GSM9050445: csf1rb_MG_uninj_3; Danio rerio; RNA-Seq
Fish were dark adapted overnight, anesthetized in tricaine, and eyes removed and placed in a tray of ice-cold PBS (phosphate buffered saline). Retinas were isolated under a dissecting microscope using fine forceps and triturated in 500 microliters of TRIzol (Invitrogen). RNA was purified according to manufacturer's directions.  A NanoDrop One spectrophotometer (ThermoFisher Scientific) was used to determine RNA concentration. Library preparation was done with the NEBNext Ultra II polyA Library Prep Kit for Illumina according to manufacturer's protocols.