RNA-Seq of Methylotuvimicrobium alcaliphilum 20Z
Cultures of Methylotuvimicrobium alcaliphilum 20Z were grown for 7 days under different environmental conditions. For RNA extraction, 6 mL of culture were harvested and centrifuged at 5000 rpm for 15 min. Total RNA was extracted using the RNeasy Mini Kit (Qiagen) with DNase I treatment (Roche). RNA samples were stored at 80 C prior to sequencing on an Illumina NovaSeq 6000 platform.