DNA-seq of Salvia rosmarinus:mature leaf
Genomic DNA was extracted and quality checked for purity, concentration, and integrity using Nanodrop, Qubit, and agarose gel electrophoresis. After quality control, the DNA was size-selected with the BluePippin system and purified using magnetic beads. DNA damage and end-repair were performed to prepare the DNA for library construction.The SQK-LSK109 ligation sequencing kit was used to attach sequencing adapters, and the library was quantified with Qubit. Finally, the library was loaded onto an Oxford Nanopore PromethION flow cell for real-time single-molecule sequencing.