Amplicon-NGS of HCV IRES: sample 624 (main genotype 1a)
libraries were made with TruSeq Nano DNA LT Library prep kit (Illumina, San Diego, CA, USA), following the manufacturers instructions. IRES PCR products obtained by One-Step PCR and purified, were quantified with Qubit 2.0 Fluorometer dsDNA HS Assay (Life Technologies, Eugene, OR, USA) before use. A paired-end (2x250) sequencing run was performed with MiSeq Reagent Kit v2 (500 cycle) (Illumina, San Diego, CA, USA) on a MiSeq instrument (Illumina).