GSM9701590: IonCode_0123_T11; Homo sapiens; RNA-Seq
RNA was prepared using the RNeasy mini kit (Qiagen, Valencia, CA, USA), and cDNA was synthesized from 2 μg of RNA using Superscript II reverse transcriptase (Invitrogen), according to the instructions of the manufacturer. Complementary DNA (cDNA) libraries were constructed from the total RNA using the Ion AmpliSeq Transcriptome Human Gene Expression kit (ThermoFisher Scientific) in accordance with the manufacturer's manuals.