HTLV-1 viral integration site detection of homo sapiens: CD34+ hematopoietic stem cells obtained from spleens of virus infected humanized mice
DNA was isolated from mouse spleen and sheared by sonication. Fragments containing the conjunction of HTLV-1 LTR and human genome were amplified by a linear extension PCR with incorporation of biotinylated dUTP, biotinylated DNA was selected with streptavidin beads and ligated to a partially double-stranded DNA linker. DNA libraries were generated by a nested PCR using primers targeting HTLV-1 LTR U5 and the linker sequence.