GSM8752864: Young female chondrocyte #2; Homo sapiens; RNA-Seq
Individual chondrocyte cultures (P0) were lysed with the QIAzol reagent (Qiagen, German Town, MD, United States), and RNA was isolated from the lysate using an RNA Easy Plus Universal Kit (Qiagen). Library preparation and sequencing were performed according to Medgenome (CA, United States). Total mRNA was processed for next-generation sequencing performed with the Illumina HiSeq 2500 system (San Diego, CA, United States). Clontech SMARTer UltraTM Low Input RNA Kits (Mountain View, CA, United States) and NexeteraXT (San Diego, CA, United States) kits were used for library preparation.