Multiplexed Assay of Variant Effect for human G6PD activity in Saccharomyces cerevisiae
Plasmid DNA was extracted from from harvested yeast pellets using Zymoprep Yeast Plasmid Miniprep II. Region containing 18bp barcodes were amplified for 5 cycles and indices and Illumina adaptors were added with an additional 10 cycles of amplification. Samples were pooled and cleaned using 1.8 ratio of AMPure XP beads.