16s rRNA sequencing of mus musculus fecal pellet
Genomic DNA was extracted from murine fecal pellets and the V3 and V4 regions of the16S rRNA were amplified using PCR. Samples were barcoded during the second round of PCR amplification. Barcoded libraries were sequenced on an Illumina platform with the 2x250 bp configuration. Extraction, amplification, library prep, sequencing, and analysis were performed by Genewiz.