GSM9230116: ABE_PDCD1_E2; Homo sapiens; OTHER
Genomic DNA extracted from primary human cells using Gentra Puregene Kit (Qiagen) according to manufacturer's protocol. Purified genomic DNA was tagmented  and circularized using a custom Tn5-transposome. Genomic DNA circles modified by base editors and selectively sequenced by end-repair and adaptor ligation for sequencing. ABE libraries were pooled and loaded on NextSeq 500 or 2000 with paired-end sequencing 151 8 8 151 cycles according to the manufacturer's instructions.