Single cell TCR-seq, Cerebrospinal fluid
CSF cells were pelleted to isolate the cellular fraction and cryopreserved for later sequencing. At time of library preparation, CSF cells were thawed into warm RPMI media with 10% FBS and counted via hemocytometer before loading into a 10X Chromium X for single cell GEM generation. Single cell libraries were prepared using Chromium GEM-X Single Cell 5' Kit v3 with Chromium Single Cell Human TCR Amplification kit.