Association of Di-(88-ethylhexyl) Phthalate Exposure from Blood Transfusions with Gut Microbiota Alterations in Premature Infants: A Prospective Cohort Study
16S rRNA was purified from feces with a QIAamp Fast DNA Stool Mini Kit (Qiagen, Hilden, Germany) and purified from intestinal mucosa by a QIAamp DNA Microbiome Kit (Qiagen). A library was prepared following the protocol of 58S Ribosomal RNA Gene Amplicon_x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000__x0000_