16S V3-V4 metagenome sequencing of adult brown planthoppers (Nilaparvata lugens) cultured on the TN1 susceptible rice variety (4th generation).)2nd replication
The project outlaid to compare the bacterial community structures in three different Brown Planthopper (Nilaparvata lugens) populations. The insect population comprised an avirulent population feeding on a susceptible rice variety (TN1) on the fourth generation using high-throughput amplicon sequencing. We predict that gut bacterial composition hosted by BPH may vary among different populations based on the  susceptible plant genotypes. Since microbiota harboured in insect gut system can regulate its adaptations depend on host plant feeding. Even the host plants itself can have impact on insects microbial community structure (2nd REPLICATION)