GSM8678807: ESC, CEBPa induced, 72h, sorted SSEA1-, clone 2; Mus musculus; ATAC-seq
Briefly, 50,000 cells were harvested, washed with PBS, and re-suspended in 50 μl of cold lysis buffer. After the centrifuge, the cell pellet was kept and proceeded for TDE1-mediated transposition reaction (15027865, Illumina) at 37°C for 30 min, followed by DNA purification using a Qiagen MinElute PCR Purification Kit (28004, QIAGEN). For library preparation, the transposed DNA fragments were amplified by PCR to add the sequencing barcodes and then purified using an AMpure reagent (A63880, Beckman). The concentration of libraries was measured by Qubit kit (Q32851, Thermo Fisher) and further diluted to 1.8 pM after denaturation. A pair-end sequencing aiming for 40 million reads per sample was run in the NextSeq instrument.