ABC
Three micrograms of RNA were used as input material for the RNA sample preparations. The mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in fragmentation buffer. Using these short fragments as templates, double-stranded cDNA was synthesized using random hexamer primers. Then, the double-stranded cDNA was subjected to end-repair and phosphorylation, and the A base was added to the 28 end. The Hieff NGS DNA Selection Beads were used for purification and fragment Selection. After purification and fragment selection, the products were amplified and enriched by PCR.