RNA-seq of shoot apices from mexican lime
Mature leaves, flowers, young fruit, and vegetative shoot apices from the same plant used for genome assembly (BioSample SAMN42593883) was used for RNA extraction with the Qiagen Rneasy Plant Mini Kit using the Sigma On-column DNase digestion kit. The NEBNext Ultra II Directional RNA Library Prep Kit was used to construct a Poly A library according to the manufacture's protocol (New England Biolabs). The library was sequenced with PE150 format by Admera Health (South Plainfield, New Jersey).