RNAseq of BMEL cell lines invalidated or not for the expression of the three HP1 isoforms (CBX1, CBX3, CBX5)
RNAseq of BMEL cell lines invalidated or not for the expression of the three HP1 isoforms (CBX1, CBX3, CBX5)
Cytosolic and chromatin-enriched fractions were obtained by sequential lysis and centrifugation steps giving a cytosolic fraction, followed by nuclei lysis and centrifugation giving a nuclear soluble fraction and a chromatin pellet.  Total RNA was extracted out of nucleosol, chromatin, or total cells by guanidinium thiocyanate-phenol-chloroform extraction followed by proteinase K and Dnase I treatment. Total RNA library preparation was performed by Novogene Co., Ltd, as a lncRNA sequencing service, including lncRNA directional library preparation with rRNA depletion (Ribo-Zero Magnetic Kit)