GSM9339666: PN7, Control; Sus scrofa; RNA-Seq
Skeletal muscle was flash frozen on dry ice, and total RNA was extracted using Trizol reagent. RNA integrity was determined with an Agilent 2100 Bioanalyzer system using the Agilent 2100 Nano 6000 Assay kit. Libraries were constructed from total RNA using the NEBNext Ultra RNA Library Prep Kit. The resulting libraries were quantified using a fluorometer and sequenced on an Illumina HiSeq-2500 platform for 150-bp pair-end reads.