GSM8889120: HEK293T cells, WT1; Homo sapiens; ATAC-seq
DNA was harvested using  DNA QIAGEN Acube HT Kit. 50000 cells were centrifuged using 4℃,5 min and 500 g centrifuging parameters, and then the supernatant was removed. The cells were washed with cold PBS once. Using 4℃,5min and 500 g centrifuging parameters the supernatant was removed.The cells were suspended with cold lysis buffer. Using 4℃,10 min and 500 g centrifuging parameters the supernatant was removed. The transposing reaction system was configured with the Tn5 Transposase. The cell nucleus were suspended with the transposing reaction system, and the DNA were purified after incubating at 37℃ for 30 minutes. The PCR reaction system was configured with the purified DNA ,and then the PCR amplification reaction was performed. The final DNA libraries were runned on sequencing platform after the DNA were purified.