ONT WGS of Mycobacterium sp. Panama NTM2
Long-read libraries were prepared using Oxford Nanopore Technologies (ONT) v14 chemistry (SQK-LSK114). To maintain DNA integrity, a sequence-independent transposome-based method was used for linearization and fragmentation in lieu of manual shearing or size selection. Sequencing was performed on an ONT PromethION platform using R10.4.1 flow cells.