GSM9848682: WT rat lung, biological replicate 3; Rattus norvegicus; RNA-Seq
Total RNA was extracted from lung tissues of WT and crTMPRSS2 rats using TRIzol™ reagent. The isolated RNA was submitted to Novogene in Munich, Germany, where RNA integrity assessment, library preparation, sequencing, and initial data processing were performed according to the provider's standard workflow. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, cDNA was synthesized using random hexamer primers, followed by second-strand cDNA synthesis, end repair, A-tailing, adapter ligation, size selection, PCR amplification, purification, library QC by Qubit/qPCR/Bioanalyzer, pooling, and sequencing on an Illumina platform. Layout: paired-end, 150 bp reads.