GSM8436245: Asymptomatic A; Homo sapiens; RNA-Seq
Peripheral blood mononuclear cells were isolated by Ficoll-Hypaque density gradient centrifugation, and isolated PBMCs were cryopreserved in liquid nitrogen according to standard protocols until use. After thawing of cryopreserved PBMC, to remove dead cells for sc-mRNAseq preparation, we took advantage of Dead Cell Removal Kit following manufacturer's instruction. 10k alive cells were then processed with 10x Technology using V3.1 kit (10x Genomics), following manufacturer's instructions. Finally library integrity was assessed by Hypersensitive (HS) Bioanalyzer run (Agilent).