GSM9558316: Mus musculus left ventricule, cKO-TFEB, Transverse Aortic Constriction, replicate 3; Mus musculus; RNA-Seq
RNA isolation from mouse left ventricular tissue was performed using TRIzol reagent and the Aurum Total RNA fatty and fibrous tissue module (Bio-Rad, 732-6870). Sequencing libraries were generated using the NEBNext Ultra RNA library prep kit for Illumina. Index-coded samples were clustered using the cBot Cluster Generation System and the PE Cluster Kit cBot-HS (Illumina). Libraries were sequenced on an Illumina platform to generate paired-ends reads.