GSM9132114: Wt H3K4me3 Rep2 6.5hpf; Xenopus laevis; OTHER
Independent experiments were performed on three different frogs, serving as three biological replicates. Within each biological replicate, 45 embryos each were collected per condition, corresponding to three replicates within each independent experiment. Embryos were collected at 6.5hpf. Nuclei were isolated from whole embryos, bound to concanavalin A beads, followed by antibody incubation with H3K4me3 or IgG as a control. Targeted digestion with pAG-MNase was performed, and soluble chromatin fragments for DNA purification were extracted using a modified CUT&RUN method. Sequencing libraries were generated using the NEB Ultra II DNA library prep kit (NEB, #E7645) as per the manufacturer's instructions using 12 PCR amplification cycles.