GSM9251583: POMC cells from PTKO;Ai9 mice, biol rep 2; Mus musculus; RNA-Seq
The collected cells were subjected to RNA extraction using a specialized reagent provided by Shi-Bio Technology (Wuhan, China), with 1-1.5 μg of total RNA used for constructing the sequencing library. The sequencing library was constructed using a modified high-activity Tn5 transposase to simultaneously fragment the DNA and add adapters to both ends of the cDNA.