scRNA-Seq of Mus musculus: Sun1-GFP+ Mller glia isolated from retina treated by FAR for 13 days
FACS-purified Sun1-GFP+ cells were processed according to Chromium 3 Gene Expression V3.1 Kit (10x Genomics, Pleasanton, CA) using manufacturers guidelinese. An estaimated 10,000 cells were loaded onto each channel with a targeted cell recovery of 6000 cells per sample. Single-cell libraries were quantified using Tapestation (Agilent) and QuBit (Thermofisher) analysis. Libraries were then sequenced in paired end mode on a NovaSeq 6000 instrument (Illumina, San Diego, CA) targeting a depth of 50,000-100,000 reads per cell.