16S seq of mouse: GCF-A
Genomic DNA is first extracted from microbial samples. The 16S rRNA gene is then amplified using specific primers targeting variable regions, followed by adding sequencing adapters and unique index sequences to the amplicons. Finally, the prepared libraries are quantified, normalized, pooled, and sequenced using a high-throughput platform Illumina MiSeq, enabling comprehensive analysis of microbial communities.