RNA-Seq of Leptolyngbya sp. FACHB-1624: after 24 h of nitrogen stepdown, biological replicate 2
Strand-specific RNA-seq libraries were prepared with 5 g of total RNA. After removing rRNA, mRNA was fragmented using the fragmentation buffer. cDNA synthesis, end repair, addition of the base A and ligation with adaptors were performed according to protocol. Libraries were then size-selected for cDNA target fragments of 200300 bp on 2% Low Range Ultra Agarose gel, followed by PCR amplification. Libraries were sequenced on Illumina HiSeq, generating 150-bp paired end reads.