Tetragonisca angustula digestive tract metagenome, sample Tang-C15.1
Amplification of the 16S rRNA V4 hypervariable region was performed using the standard 515F/806R primer pair (GTGCCAGCMGCCGCGGTAA, GGACTACHVGGGTWTCTAAT). Amplicon amplification, library preparation and sequencing were performed by Novogene Corporation (Sacramento, CA, USA), generating paired-end reads of 250 bp (PE250) per sample on an Illumina NovaSeq 6000 platform.