GSM8712005: OH14654, dauer, solvent control, rep2; Caenorhabditis elegans; RNA-Seq
24,000 dauer stage animals per sample were lyzed in TRIzol reagent and total RNA was extracted using RNeasy Micro Kit (Qiagen) using the manufacturer's protocol. 100 ng of total RNA per sample was used for library prep using Universal RNA-seq with NuQuant kit (Tecan) using the manufacturer's protocol with custom AnyDeplete (IC0149S) to deplete rRNA.