GSM9340201: HCT116, Tram, 24h, rep2; Homo sapiens; RNA-Seq
RNA was isolated from cultured cells using the peqGOLD Total RNA Kit (VWR), following the manufacturer's instructions. After lysis in TRK buffer and homogenization, lysates were applied to RNA Mini Columns. DNase I digestion was performed to remove genomic DNA. RNA was washed, eluted with preheated nuclease-free water (70 °C) and stored at −70 °C. Libraries were prepared at the DKFZ Genomics & Proteomics Core Facility using the TruSeq Stranded mRNA Library Prep Kit (Illumina) and sequenced on a NovaSeq 6000 (Illumina) as paired-end 100 nt long reads.