GSM8450394: mouse eyelid, 8 weeks wildtype [Spatial transcriptomics]; Mus musculus; OTHER
Eyelids from an 8-week-old male C57BL/6J mouse  was dissected, OCT embedded, sectioned at 10 μM, and mounted onto the assay slide containing four capture areas (6.5mm x 6.5mm) with spatially barcoded poly T capture probes. After permeabilization, the captured mRNAs underwent reverse transcription, followed by cleaving from the slide. cDNA amplification, fragmentation, end-repair, poly A-tailing, adapter ligation, and sample indexing were performed in accordance with the manufacturer's protocol.