WGS of native Arabidopsis thaliana
The genomic DNA was randomly sheared. The fragments were end-repaired, A-tailed, and further ligated with Illumina dual indexed adapters. The resulting fragments with adapters were size selected and PCR amplified before proceeding for purification. The library was quantified through Qubit and qPCR, and size distribution estimated with a fragment analyzer. Quantified libraries were pooled and sequenced.