The effects of CDK4/6 inhibitors on chemotherapy-induced senescent cells
The effects of CDK4/6 inhibitors on chemotherapy-induced senescent cells
For each sample, 5000 cells were used for the library preparation, aiming to capture approximately 2500 cells. 3 groups of BJ fibroblasts (population doubling 32) were treated as below:  1. V+V — Vehicle (water for 24 hours) + Vehicle (water for 2 times 24 hours) at 6 days post 1st treatment.  2. D+V — Doxorubicin (250nM for 24 hours) + Vehicle (water for 2 times 24 hours) at 6 days post 1st treatment.  3. D+A — Doxorubicin (250nM for 24 hours) + Abemaciclib (1uM for 2 times 24 hours) at 6 days post 1st treatment. Single cells were captured using SeekOne® Digital Droplet System (Beijing SeekGene BioSciences Co., Ltd) according to the manufacturer protocol.  Single cell RNA-seq libraries were prepared using SeekOne® DD Single Cell 3' Transcriptome-seq Kit (Beijing SeekGene BioSciences Co., Ltd) according to the manufacturer protocol.