B6 27mo inf. infected d9 3
Total RNA was extracted from the lungs of B6 mice and used to construct strand-specific RNA-seq libraries. Poly(A) RNA was enriched using oligo-dT magnetic beads, followed by fragmentation and reverse transcription. Second-strand synthesis incorporated dUTP to maintain strand specificity. Libraries were constructed using the NEBNext Ultra II Directional RNA Library Prep Kit, following the manufacturers protocol. Paired-end sequencing (2  150bp) was performed on an Illumina NovaSeq 6000 platform by Novogene (Beijing, China).