Metagenome of UC affected individual 285
Libraries were constructed using the Illumina DNA Prep (M) Tagmentation Kit (Illumina, 20018705) with IDT for Illumina DNA/RNA UD Index Sets A-D (Illumina 20027213-16) according to manufacturers instructions, with a modification of volume to accommodate processing in a 384 plate format. Resulting libraries were assessed using a high sensitivity dsDNA fluorometric assay (QuantIT, ThermoFisher, Q33120) and individual libraries were visualised with capillary gel electrophoresis using the QIAxcel DNA High Resolution Kit (Qiagen, 929002). Libraries were required to meet minimum criteria for average size, smallest and largest fragment gating and concentration.