chromosome sequence of Synechocystis sp. PCC 6803 WT 5
Genomic DNA libraries were prepared using the TruSeq DNA PCR-Free Library Prep Kit (Illumina) following the manufacturers protocol. Briefly, DNA was sheared to an average insert size of either 350 bp or 550 bp using Covaris ultrasonication, generating double-stranded fragments with 3 or 5 overhangs. Fragmented DNA underwent end repair and size selection, followed by 3 adenylation. Indexed adapters were ligated to the A-tailed DNA fragments. The libraries were then purified and quality-checked for concentration and fragment size distribution. Libraries were normalized using 10 mM Tris-HCl (pH 8.5) containing 0.1% Tween 20 and subsequently pooled for sequencing. Sample information, including indexing and pooling design, was managed using Illumina Experiment Manager (IEM) and BaseSpace Prep Tab to ensure balanced index combinations according to the Index Adapter Pooling Guide (Illumina, document #1000000041074)