16S rRNA of Surface water : Owena Dam
Genomic DNA samples were PCR amplified using a universal primer pair 27F and 1492R - targeting the V1 -V9 region of the bacterial 16S rRNA gene. Resulting amplicons were barcoded with Pacbio M13 barcodes for multiplexing through limited cycle PCR. The resulting barcoded amplicons were quantified and pooled equimolar and AMPure PB bead-based purification step was performed. The PacBio SMRTbell library was prepared from the pooled amplicons following manufacture protocol. Sequencing primer annealing and Polymerase binding were done following SMRTlink Link software protocol to prepare the library for sequencing on PacBio Revio system.