GSM8819796: Testis, Tent5c wt/wt, busulfan, rep2; Mus musculus; OTHER
Total RNA was isolated from total testis using TRIzol. Poly(A) RNAs were purified with oligoT beads. Direct RNA sequencing libraries were built according to the Oxford Nanopore Technologies protocol (Direct RNA Sequencing Kit- SQK-RNA002) with modifications to incorporate barcoded oligonucleotides for multiplexing. This analysis include 2 libraries considered here as replicate (rep1 and rep2). Each library was prepared by multiplexing 4 mRNA extracts (BC 1-4) representative of 1 mouse in each of the 4 conditions. Libraries were run on Nanopore R9 chips with a GridIon instrument.