GSM8625112: cerebellar cells, KO male mouse; Mus musculus; RNA-Seq
Cerebellar cells were obtained from mouse cerebella (P5) through the meticulous process of cerebellum dissection, cell straining, and plating. Two to four of cerebella were pooled for each genotype (Kmt2d fl/fl and Atoh1-Cre Kmt2d fl/fl mice) per gender. Single cells were isolated using flow cytometry for library preparation. Cells were individually isolated and barcoded using droplet-based technique (Chromium Next GEM Single Cell 3ʹ Kit v3.1), and the transcripts were collected.