RNA-Seq of Streptococcus mutans UA159:cell culture
After removal rRNA, cDNA synthesis, and adaptor ligation, DNA fragments with ligated adaptor molecules on both ends were selectively enriched using Illumina PCR Primer Cocktail via PCR (15 cycles). PCR products were purified (AMPure XP system) and quantified using Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system (Agilent). The library was sequenced on HiSeq sequencing platform (Illumina).