RNA-seq of mouse: ESCs
The total RNA of tissue and cell was extracted by TRIzol reagent (TaKaRa, Tokyo, Japan). The quality control of RNA samples and the removal of ribosomal RNA were performed by NanoPhotometer spectrophotometer and the RiboMinuTM Eukaryote Kit (Thermo Scientific, USA), respectively. The RNA samples were sequencing with the Illumina or DNBSEQ.