Silkworm fat body transcriptome
Total RNA was reverse-transcribed and amplified into double-stranded cDNA using the SMART-Seq v4 Ultra Low Input RNA Kit. The resulting cDNA was purified, quantified, and fragmented via Tn5 transposase tagmentation. Fragments were PCR-enriched using indexed primers (S5XX and N7XX), size-selected to 400500 bp, and purified. Library quality and effective concentration were assessed using a LabChip GX system, Qubit fluorometer, and qPCR.