Whole genome sequencing for sudden unexplained infant death
Whole genome sequencing was performed at the Human Genome sequencing center (HGSC) at Baylor College of Medicine. Following sample QC, libraries were prepared with KAPA Hyper pcr-free reagents (Roche# KK8505) and the final library size estimation and quantification are done using the Fragment Analyzer (AgilentAdvanced Analytical Technologies, Inc) electrophoresis system and  QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems™) respectively. Libraries were sequenced using the Illumina NovaseqX to generate 150 bp paired-end sequence reads for all samples in a format of multiplexed pools to generate an average of 30X coverage. Post-sequencing data analysis was performed using Illumina's DRAGEN pipeline. To ensure sample identity and integrity the Fluidigm SNPtrace method for rapidly genotyping 96 SNP sites was employed to verify gender prior to sequencing and to detect contamination. Using this assay, sample identity was verified using the Error Rate In Sequencing (ERIS) software developed at the HGSC.