GSM8483032: BM-MSC, M-Ti; Homo sapiens; RNA-Seq
Total RNA samples of BM-MSC cultured on SiN, MTi and ATi discs for 21 days were extracted using Quick RNA Miniprep kit (Zymo Research, Inc. Irvine, CA). Total RNA samples from 3 separate wells in the group were combined at the equal quantity. Libraries for RNA-Seq were prepared with KAPA Stranded mRNA-Seq Kit. The workflow consists of mRNA enrichment and fragmentation, first strand cDNA synthesis using random priming followed by second strand synthesis converting cDNA:RNA hybrid to double-stranded cDNA (dscDNA), and incorporates dUTP into the second cDNA strand. cDNA generation is followed by end repair to generate blunt ends, A-tailing, adaptor ligation and PCR amplification. Different adaptors were used for multiplexing samples in one lane.