Genome sequence of M. feriruminatoris hsdR-::LP mutant
High molecular weight DNA was sheared with Megaruptor (Diagenode) to obtain 10 kb fragments. DNA size distribution was checked on a Fragment Analyzer (Advanced Analytical Technologies) and 500 ng of each DNA sample were used to prepare a SMRTbell library with the PacBio SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences). The resulting libraries were pooled with other libraries processed in the same way. The pool was size selected with Ampure PacBio beads to eliminate fragments <5 kb. Sequencing was performed with v3.2/v2.0 chemistry and diffusion loading on a PacBio Sequel II instrument (Pacific Biosciences) at 900 min movie length and pre-extension time of 120 min using one SMRT cell 8M.