GSM8888965: PBMC_Severe_Patient_pool; Homo sapiens; RNA-Seq
PBMCs were isolated from patient blood samples using  Ficol. Isolates PBMCs were washed twice with 0.5% BSA/PBS. Each sample pool was loaded into a different lane of a 10× chip (Single Cell G Chip Kit for v3.1). The 10x Chromium controller (10x Genomics), in combination with v3.1 reagents, was used to capture the single cells and generate Single-Indexed sequencing libraries, according to the manufacturer's instructions (document CG000204, RevD).Sequencing was performed with a 150 bp paired-end kit using a custom program (28-8-0-150) on the Illumina NextSeq 500/550.