GSM8927575: A549 cells, TRA2B KD, replicate 3; Homo sapiens; RNA-Seq
Total RNA was extracted from cells using the Maxwell RSC simplyRNA Cells Kit (Promega, AS1390) and the Maxwell RSC48 Instrument (Promega). RNA-seq library preparation was performed by Novogene. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, first strand cDNA was synthesized using random hexamer primers. Then the second strand cDNA was synthesized using dUTP. The directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, PCR amplification, and purification.