GSM9288031: PACE076; Homo sapiens; RNA-Seq
Peripheral whole blood RNA was collected into PAXgene Blood RNA tubes (Becton Dickinson and Company, Franklin Lakes, New Jersey) and stored at −80 C. Automated RNA extraction was performed using the QIAsymphony PAXgene Blood RNA Kit (PreAnalytiX, Qiagen/BD). RNA yield and integrity were evaluated prior to sequencing. Library preparation was performed with the Illumina TruSeq kit (Illumina, San Diego, CA) using 200 ng of total RNA per sample as input. Libraries were amplified for 12 PCR cycles, then quantified, normalized, and pooled for sequencing. Sequencing was carried out on an Illumina HiSeq 4000 platform (v4 chemistry, single-read configuration).