GSM9557560: Huh7 cells  SR2; Homo sapiens; RNA-Seq
Total RNA was extracted with the Cell RNA Rapid Extraction Kit from GOONIE (400-100-100T) according to the manufacturer's instructions. RNA libraries were constructed using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB) following the manufacturer's protocols. Briefly, mRNA was enriched using oligo(dT) beads, fragmented, and used for first-strand and second-strand cDNA synthesis. After end repair, A-tailing, and adapter ligation, the products were purified and PCR amplified to create the final library.