smallRNAseq from one plant sample showing necrotic leaves and collectedat Bern de Astrada, Corrientes province: First description of RNVM in Argentina
The study aims to investigate viruses present in rice (Oryza sativa) in Argentina. Twelve symptomatic leaf samples were collected from rice fields in Corrientes and Santa Fe provinces in 2018. Viral nucleic acids were extracted and Illumina HiSeq sequencing (VANA approach) was performed to identify viruses associated with disease symptoms. Subsequently, small RNA sequencing was carried out on total RNA from a representative sample (Arg1) to reconstruct the complete genome of a rice necrosis mosaic virus (RNMV) isolate and to investigate the small interfering (si)RNAs produced by the plant in response to the viral infection.