Integrative Whole-Genome and Epigenome profiling of cfDNA in Familial Prostate Cancer: Insights from a Pilot Study
Integrative Whole-Genome and Epigenome profiling of cfDNA in Familial Prostate Cancer: Insights from a Pilot Study
Peripheral whole blood samples were collected from eight patients with familial prostate cancer using Cell-Free DNA BCT tubes (Streck, La Vista, NJ, USA) according to the manufacturer's protocol Cell-Free DNA (cfDNA) was extracted using the QIAamp Circulating Nucleic Acid Kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol. Indexed libraries were prepared from 10 ng purified total cfDNA each using Biomodal duet evoC kit (Biomodal, Chesterford Research Park, Cambridge, United Kingdom) was carried out according to the manufacturerâ€™s instructions.