Shotgun metagenomic sequencing of gastric lavage from Eublepharis macularius
Shotgun metagenomic sequencing was performed from total DNA extracted from a gastric lavage sample collected from a pet leopard gecko (Eublepharis macularius) during clinical investigation in Brazil. DNA was extracted using the MagMAX Viral/Pathogen Kit, quantified with the Qubit dsDNA HS Assay, and libraries were prepared with the Illumina COVIDSeq kit starting from the tagmentation step. Sequencing was carried out on an Illumina MiSeq platform using paired-end reads.