Lungs_F_3
RNA was isolated from tissues using the RNeasy mini kit (Qiagen #74534) according to manufacturers protocol. RNAseq cDNA libraries were prepared from 1 g total RNA using the Illumina TruSeq stranded mRNA kit (Cat. No. RS-122-2101) according to the manufacturer's instructions.After elution, libraries were quantified using the Qubit 4 Fluorometer (ThermoFisher Scientific, #Q33238) using the Qubit 1X dsDNA Assay Kit (ThermoFisher Scientific, #Q32853).