GSM8626335: GFP18-4; Mus musculus; RNA-Seq
RNeasy Mini Kit (Qiagen) according to the manufacturer's protocol 250 ng RNA was used for ribosomal RNA depletion using the NEBNext rRNA Depletion Kit (Cat# E6310X) according to the manufacturer's instructions. Double-stranded cDNA was then synthesized using the NEBNext Ultra II RNA First Strand Synthesis (Cat# E7771) and the NEBNext Ultra II Directional RNA Second Strand Synthesis (Cat# E7550L) modules. Libraries were constructed using NEBNext Ultra II End Repair/dA-Tailing (Cat# E7546L) and NEBNext Ultra II Ligation (Cat# E7595L) modules. Each library was amplified for 9 PCR cycles using NEBNext High-Fidelity 2X PCR Master Mix (Cat# M0541L) and NEBNext Multiplex Oligos for Illumina (UDI) (Cat# E6440S)