GSM8626655: 15, C57Bl6/J: PD1KO, WT, T cells, ADT, HTO; Mus musculus; RNA-Seq
Lymphocytes were prepared by either digestion with liberase (submandibular gland) or mechanical dissociation (spleen) , followed by staining with fluorescent antibodies, hashing with Totalseq-C antibodies against MHCI and CD45,  and flow sorting CD3+ T cells into DMEM+ 2%FCS. Cells were stained using a custom Totalseq-C antibody panel then sorted again into DMEM + 2% FCS Libraries were prepared according to the 10x Genomics 5'v2 protocol for GEX, VDJ, and FBC