GSM8595399: J-Lat 10.6, shCD8B, AcH4, ChIP, unstimulated, Rep1; Homo sapiens; ChIP-Seq
Native ChIP was performed using MNase to fragment chromatin to mono-to pentanucleosomes sizes. ChIP DNA libraries for next-generation sequencing on the Illumina platform were constructed based on the protocol of NEB Next Ultra II DNA Library Prep Kit (#E7103, NEB) using NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs, # E6440S)