GSM9203326: OB-0d; Homo sapiens; ncRNA-Seq
Total RNA was extracted using Trizol reagent. High throughput sequencing service was provided by CloudSeq Biotech (Shanghai, China). Total RNA of each sample was used to prepare the tRF&tiRNA sequencing library, which included the following steps: 1) 3'-adaptor ligation; 2) 5'-adaptor ligation; 3) cDNA synthesis; 4) PCR amplification; 5) Size selection of ~150 bp PCR amplicons The libraries were denatured as single-stranded DNA molecules, captured on Illumina flow cells, amplified in situ as clusters and finally sequenced for 50 cycles on Illumina HiSeq sequencer following the manufacturer's instructions.