GSM9251677: RNA-seq of MCF-7 negative control replicate 1; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol reagent (Invitrogen, USA) according to the manufacturer's instructions. RNA quality and integrity were assessed using Agilent 2100 Bioanalyzer.the Illumina NovaSeq 6000 platform to generate paired-end 150 bp reads.l RNA-seq libraries were prepared using the Illumina TruSeq Stranded mRNA Library Prep Kit following the manufacturer's protocol. Libraries were quantified by Qubit and sequenced on the Illumina NovaSeq 6000 platform to generate paired-end 150 bp reads.