GSM9157945: 129CAS_MN_2; Mus musculus; RNA-Seq
motor neurons were processed using Rneasy Micro Kit (Qiagen); samples were treated on-column with RNase-Free DNase Set (QIAGEN, Hilden, Germany) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.