RNA-seq of plerocercoid Ligula intestinalis from Abramis brama
RNA extraction was performed from 1x1 mm tissue fragments from the head, body center and tail of the worm body. Total RNA was purified with Biolabmix columns. mRNA was enriched with NEBNext Poly(A) mRNA Magnetic Isolation Module for Illumina, and libraries were prepared with a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina and sequenced with a NovaSeq 6000 device.