Macrogenics
Sample DNA extraction and fragmentation: approximately 350 bp. Construct paired-end (PE) libraries and perform sequencing: 1) Add modified DNA polymerase and dNTPs labeled with four fluorescent dyes, synthesizing only one base per cycle; 2) Scan the reaction plate surface with a laser to read the nucleotide types incorporated during the first round of reaction for each template sequence; 3) Chemically cleave the fluorescent group and terminator group to restore 3'-end stickiness, then continue synthesizing the second nucleotide; 4) Compile the fluorescence signal results collected per round to determine the sequence of the template DNA fragment.