GSM8754024: AGS Compound-A 3; Homo sapiens; RNA-Seq
All cell samples were extracted by Trizol  first and then use Rneasy Mini Kit for RNA purification Novogen company perform the sample quality check (QC) using (1) 1% Agarose gel electrophoresis, (2) Nanodrop reading to check for RNA amount and purity, and (3) Agilent2100 to check for RNA Integrating Number (RIN). At Novogene, they deploy each of these three methods to check the quality of the data you send to us, thereby ensuring you work only with the highest quality data. Once  confirm the quality of the extracted total RNA is good,  and  move on with the protocol and perform the Library Construction step. The polyA enrichment library preparation procedure is most commonly used when working with mRNA-Seq data and results in the construction of 250-300 bp insert cDNA library.