GSM9290012: larval guts, yw (GF), Early L3 (S1), replicate B; Drosophila melanogaster; RNA-Seq
RNA was extracted using the NucleoSpin kit (Macherey Nagel, #740955) following the manufacturer's protocol, and quantified using the Nanodrop spectrophotometer (Thermofisher, ND-2000). For RNA sequencing, samples were treated by the IGFL sequencing platform. Purification, quantification and quality checks were performed using the Qubit 2.0 – RNA BR Assay Kit (Thermofisher) and the TypeStation 2200 (Agilent). Libraries were generated following the online protocol (https://www.hexabiogen.com), quantified via the TapeSation D1000 ScreenTape Assay (Agilent), and the raw data were subsequently processed to generate final genes regulation datasets