GSM9130981: lung, RHL2; Mus musculus; RNA-Seq
Total RNA was extracted from mouse lung tissues using TRIzol reagent (Invitrogen) according to the manufacturer's instructions, followed by purification using the RNeasy Mini Kit (Qiagen). RNA integrity was evaluated with an Agilent 2100 Bioanalyzer. RNA-seq libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB), according to the manufacturer's protocol. Polyadenylated mRNA was enriched using oligo(dT) magnetic beads, fragmented into ~200 bp fragments, and reverse-transcribed into first-strand cDNA. Second-strand cDNA synthesis, adaptor ligation, and PCR amplification were performed to complete the library. Libraries were sequenced on a DNBSEQ-G400 platform (MGI Tech Co., Ltd.).