GSM9598702: YUMM5.2 tumor cells from C57BL/6 mice treated with dabrafenib/trametinib (D/T); Mus musculus; RNA-Seq
Tumors (n=5/group) were pooled, dissociated with a MACS dissociator (mouse melanoma setting), red blood cells lysed and dead cells removed (Miltenyi kits). Viability was assessed on a Countess II FL. Library preparation, sequencing, and analysis was performed by the Markey Cancer Center Oncogenommics Shared Resource. Live cells (D/T=10,251; D/T+nilotinib=15,947) were targeted for Gel Beads-in-emulsion (GEMs) formation on the 10X Genomics Chromium Controller. Library preparation was conducted according to the manufacturer's instructions using Chromium Next GEM Single Cell 3' Gene Expression Kit v3.1.