ONT SQK-NBD114-96 library of Stenotrophomonas STK16_22: R10.4.1 flowcell on PromethION, guppy v7.2.13 with model dna_r10.4.1_e8.2_400bps_sup@v4.2.0
The library was prepared using the SQK-LSK114 ligation sequencing kit (Oxford Nanopore Technologies [ONT]) with native barcoding and sequenced on a PromethION platform using a R10.4.1 flow cell. Base calling and demultiplexing were performed using Guppy v7.2.13 with model dna_r10.4.1_e8.2_400bps_sup@v4.2.0 in super high accuracy mode. Reads were trimmed with cutadapt -e 0.2 --trimmed-only -g AAGGTTAANNNNNNNNNNNNNNNNNNNNNNNNCAGCACCT followed by cutadapt -e 0.2 -a AGGTGCTGNNNNNNNNNNNNNNNNNNNNNNNNTTAACCTTAGCAAT -m 1000