GSM8678094: TNBC3; Homo sapiens; RNA-Seq
After surgery, TNBC samples were chopped into submilimeter particles manually and incubated for 30 minutes in a 37℃ 5% CO2 incubator with RPMI-160, supplemented with 1mg/mL hyaluronidase, 1mg/mL collagenase type IV, and 0.5mg/mL DNase I. After tissue digestion, cells were filtered with 70um cell strainers. Erythrocytes were lysed with 3ml ACK lysis buffer on ice for 5 min. The reaction was stopped by adding 10ml 1X PBS. After centrifugation, cells were resuspended with 1ml RPMI-1640 supplemented with 10% FBS. CD11b+ cells were isolated with BD Aria III sorter, and then were immediately sent for scRNA-seq. Library was performed according to the manufacturer's instructions.