GSM9203352: U87-R, STM2457, rep 2; Homo sapiens; RNA-Seq
Cells were lysed in Trizol and RNA was extracted by pehnol/chloroform extraction as per manufacturer instructions, followed by isopropanol precipitation. For U87 MG cell lines, 90ug of total RNA was used for polyA enrichment using the Dynabeads mRNA DIRECT Purification kit (Invitrogen), followed by fractionation using RNA Fragmentation Reagents (Invitrogen). For U87 MG cell lines, libraries were prepared using the TruSeq Stranded mRNA Library Prep (Illumina) from 30ng fragmented mRNA. For LN229 cell lines, libraries were prepared using the Watchmaker mRNA Library Prep kit from 1ug of total RNA.