RNA-Seq of cyanobacteria: monoculture Tihonema sp.
Libraries of 16S rRNA gene fragments were generated by PCR using universal primers for the V4 variable region: F515/R806 GTGCCAGCMGCCGGCGGTAA/GGACTACVSGGGTATCTAAT. Library preparation and sequencing were performed according to the manufacturer's recommendations for the Illumina MiSeq instrument (Illumina, San Diego, CA, USA) using the MiSeq ReagentKit v3 (600 cycles) in a paired-pair cycle (2  300 nt). The DADA2, PHYLOSEQ, and SILVA software packages were used for taxonomic classification of the resulting OTUs.