GSM9291741: strain BPK275 promastigotes logarithmic Replicate 2; Leishmania donovani; RNA-Seq
The total RNA was extracted using the Quick-RNA Microprep Kit (R1050, ZYMO RESEARCH) following the manufacturer's instructions. The RNA's quantity and quality were evaluated using the Qubit RNA Quantification Assay Kit broad-range (Thermo Fisher) and RNA Screen tape system (Agilent). The RNA-Gene Expression (poly-A selection) library preparation and sequencing service were performed by GenomeScan (Netherlands) on an Illumina NovaSeq6000 platform (2 x 150 bp paired-end reads) with a sequence depth of ~20 M PE reads/sample.