GSM9669423: GEM2; Homo sapiens; RNA-Seq
RNA was harvested with 10X genomics Single-cell protocols. Single-cell dissociated liquid nitrogen-stored samples were resuspended and counted using Luna automated cell counter. A total of 20,000 cells were loaded per channel on a Chromium X using Chromium Next GEM Single Cell 5' v2 reagents (10X Genomics, Pleasanton, CA, USA), and scRNA-seq libraries were prepared according to the manufacturer's instructions. The libraries were analyzed and quantified using TapeStation D1000 screening tapes (Agilent, Santa Clara, CA, USA) and sequenced using the NovaSeq 6000 system (Illumina, San Diego, CA, USA).