GSM8754064: Fibroblasts-derived extracellular vesicles, FB-EVs sample3; Homo sapiens; miRNA-Seq
Total RNA was extracted by TRIzol The RNA molecules in a size range of 18–30nt were enriched by polyacrylamide gel lectrophoresis(PAGE). Then the 3' adapters were added and the 36-44nt RNAs were enriched. The 5' adapters were then ligated to the RNAs as well. The ligation products were reverse transcripted by PCR amplification and the 140-160bp size PCR products were enriched to generate a cDNA library and sequenced using Illumina HiSeqTM 2500 by Gene Denovo Biotechnology Co.