DNA-seq of bacteria
For PacBio library preparation, genomic DNA is fragmented into ~10 kb fragments. End repair is subsequently performed per the PacBio manufacturer's instructions (Pacific Biosciences, CA), and circular single-stranded adapters are ligated to both ends of the fragmented DNA. Each single-stranded adapter is ligated to the positive and negative strands of the double-stranded DNA fragment, generating a dumbbell-shaped ("hairpin loop") structure termed SMRT Bell.