GSM8889440: PDAC-CA-Tumor-25; Homo sapiens; RNA-Seq
Total RNA (including small RNAs) was extracted from FFPE tissue sections using Qiagen AllPrep DNA/RNA FFPE kits (Cat #80234), following the manufacturer's protocol. RNA integrity and quantification were assessed using the Agilent TapeStation instrument with High Sensitivity RNA ScreenTapes (Cat #5067-5579) and Qubit RNA assay kits (Cat#: Q32855, Invitrogen, Thermo Fisher). Library preparation from input RNA was performed using SureSelectXT Low Input Library Preparation and Target Enrichment Kits (Cat #5190-9686). Final pooled libraries (12-plex) were run to obtain 30M read depth for single-end reads using NextSeq 500/550 High Output Reagent Cartridge v2 (Cat # 15057934) on an Illumina NextSeq 500/550 instrument.