GSM9287969: Replicate 1, GFP reporter C50 cell, GFP positive, dDAVP; Mus musculus; RNA-Seq
RNA was extracted using Direct-zol RNA MicroPrep kit Trizol-extracted RNA was proceeded to cDNA generation using the SMART-Seq HT Kit (Takara Bio). cDNA was generated using 1 ng of total RNA with 11 PCR cycles. 1 ng of the resulting cDNA was “tagmented” and barcoded by using the Nextera XT DNA Sample Preparation Kit (Illumina) to generate sequencing libraries for high throughput sequencing. Libraries were purified by AmPure XP magnetic beads (Beckman Coulter), and quantified using a Qubit 2.0 Fluorometer (Thermo Fisher Scientific). An equal amount of index libraries were pooled and sequenced (paired-end 50 bp) on an Illumina platform.