GSM9293290: A549_IAV_mock_2; Homo sapiens; RNA-Seq
Cells were washed with PBS, and lysed in RLT buffer (Qiagen, Germany). Total RNAs were isolated with the RNeasy Mini Kit (Qiagen) and treated with DNase I (Qiagen) following the manufacturer's instructions. RNA samples were stored at -80°C and used for preparation of libraries for RNA sequencing. RNA sequencing libraries were prepared using an Illumina Stranded mRNA Prep library preparation kit (Illumina, San Diego, CA, USA) in accordance with the manufacturer's instructions.