WGS data of Klebsiella pneumoniae isolate - HLVUKPBL14, obtained from the blood sample of a critically infected patient.
The fundamental principle of Illumina sequencing technology revolves around the utilization of fluorescently labeled nucleotides that possess reversible terminators. This approach shares the core concept of """"sequencing by synthesis-as-you-go"""" akin to the Sanger method. However, unlike Sanger, this technique involves temporary termination of DNA strand extension after incorporating each uniquely modified nucleotide. Once the added nucleotides are optically detected using specific fluorescent markers, the terminator molecules are cleaved, allowing the synthesis of the new strand to resume for the subsequent round of nucleotide addition.