microbiome of potato rhizocompartments
Total genomic DNA from the rhizosphere, rhizoplane, and free soil was isolated using a QIAamp Fast DNA Stool Mini Kit (QIAGEN, Germany) according to the manufacturer's instructions. DNA quality and concentration were assessed using 1% agarose gel electrophoresis and a NanoDrop 200 UV spectrophotometer (Thermo Scientific, USA). The isolated DNA was stored at -20C until further PCR amplification. DNA was amplified using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB, UK).