GSM9848154: DE+PFD2; Mus musculus; RNA-Seq
Total RNA was extracted from the lung tissue stored in RNAlater using the TRIzol reagent method. 1000ng of total RNA was used for the construction of libraries. Oligo(dT) beads were used for capturing the polyadenylated mRNA and rRNA, and other non-mRNA was washed away. The mRNA was fragmented and reverse-transcribed to synthesise first- and second-strand cDNA. The adapters were ligated to the ends of the synthesized strands, and strand-specific PCR amplification was performed. The quality (size distribution and concentration) of amplified libraries was checked before sequencing.