GSM9905797: Pooled liver nuclei from young WT, aged WT, aged ADK control, and aged ADK heterozygous mice; Mus musculus; RNA-Seq
Nuclei were isolated from frozen mouse liver tissue using a nuclei isolation procedure optimized for single-nucleus RNA sequencing. Isolated nuclei were filtered, counted, and assessed for quality before processing. Single-nucleus gene-expression libraries were prepared using the 10x Genomics Chromium Single Cell Gene Expression Flex workflow according to the manufacturer's instructions. The four samples were labeled with distinct sample-specific probe barcodes, pooled, and sequenced as one multiplexed paired-end library.