GSM9521349: Colon tissue, loperamide-induced constipation + Rothia mucilaginosa gavage, biol rep 4; Mus musculus; RNA-Seq
Total RNA was extracted from colon tissue samples using standard extraction methods. RNA integrity was assessed using the Agilent 2100 Bioanalyzer to ensure sample quality before library construction. mRNA was enriched from total RNA using oligo(dT) magnetic beads. After fragmentation, the first-strand cDNA was synthesized using random hexamer primers. After second-strand cDNA synthesis, end repair, dA-tailing, and adaptor ligation were performed. The library was amplified by PCR and quality-checked using an Agilent 2100 Bioanalyzer. Libraries were sequenced on the Illumina NovaSeq 6000 platform to generate 150 bp paired-end reads.