GSM8990866: Bulk RNAseq, MEF cells, Nontargeting gRNA, Day 6, biol rep 2; Mus musculus; RNA-Seq
Total RNA extracted using the Quick-RNA Microprep Kit (Zymo Research). RNA integrity was checked with High-sensitivity RNA Screen Tape and Reagents (Agilent); RINe values were > 8.5 for all samples Sequencing libraries were prepared using the NEBNext Single Cell/Low Input RNA Library Prep Kit (NEB) according to the manufacturer's instructions