GSM8888683: Western Diet 5; Mus musculus; RNA-Seq
Isolated mouse atherosclerotic aortas were stored in RNA later (Cat.# AM7021, Invitrogen) for 24 hours, then tissue was stored at -80 C until processed for RNA extraction using the gentleMACSTM Octo Dissociator (Miltenyi Biotec) homogenization protocol for total RNA isolation, the QIAzol Lysis Reagent (Qiagen, Cat.# 79306) and RNA clean-up using the RNAeasy Mini kit (Qiagen). Sample quality and quantity was determined with Agilent 2100 bioanalyzer, using the Agilent RNA 6000 Nano Kit (Agilent Technologies, Cat.# 5067-1511). For RNA-sequencing, 250ng of RNA were used as input for poly-A library construction. Transcriptome Library - non-stranded mRNA sequencing on DNBseq platform