Zebra finch Gonadal culture timecourse, DIV5 replicate 1
Non-adherent fractions of zebra finch gonadal cultures were collected, and RNA was extracted using RNA micro kit (QIAGEN, Cat. # 74034), reverse transcribed using SuperScript Reverse Transcriptase II (Invitrogen, Cat. # 18064014), and PCR amplified for 16 cycles with KAPA Hi-Fi HotStart ReadyMix (KAPA Biosystems, Cat. #KK2601). Libraries were prepared using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB, Cat. #E7805S) before being sent to Novogene Corporation, Inc. (Sacramento, CA, USA) for sequencing on an Illumina HiSeq 4000.