Amplicon sequencing of ITS2 region from environmental DNA
Amplicons were generated using the following primer sequences: ITS: ITS_1F CTTGGTCATTTAGAGGAAGTAA, ITS_2 GCTGCGTTCTTCATCGATGC; The library was sequenced on the Illumina NextSeq2000 P1 600cycle, generating 2x301 bp reads.