stool microbiome sequencing
A single aliquot of a fecal specimen was placed into a Qiagen PowerBead glass 0.1 mm tube and a Promega Maxwell RSC PureFood GMO and Authentication Kit was utilized to isolate DNA. The tube was vortexed, incubated at 95°C for 5 minutes, vortexed at high speed for 20 minutes, and centrifuged at 12,700 rpm for 10 minutes. The tube was subsequently loaded into a Promega MaxPrep Liquid Handler tube rack and DNA was isolated using a Promega MaxPrep Liquid Handler. Library generation was performed using the Illumina Nextera XT DNA Library Prep Kit.