GSM8595545: control, replicate1, scRNA-seq; Mus musculus; RNA-Seq
Mouse lung tissue samples from two ALI and control mice each were minced and digested by collagenase II and trypsin for single cell suspensions, which were loaded onto ChromiumTM microfluidic chips (viability ≥80%, fragmentation rate ≤5%, group rate ≤5%). The released mRNA was reverse-transcribed into complementary deoxyribonucleic acid (cDNA) with barcode. Subsequently, the commercial 10x Genomics platform (10x Genomics, Pleasanton, USA) was used for the establishment of cDNA libraries. Following the 10x Genomics's instructions, technician generated the scRNA sequencing libraries by Single-Cell 3' Library Kit v3 which were sequenced on a NovaSeq 6000 system (Illumina, San Diego, US) to obtain 150 bp at each end.