GSM9813523: Male mouse endothelial cells, vehicle control, biol rep 3; Mus musculus; RNA-Seq
Total RNA was isolated in-house using a TRIzol-based extraction method. Cells were lysed directly in TRIzol reagent, and RNA was purified by chloroform extraction and isopropanol precipitation. RNA pellets were washed with 75% ethanol, resuspended in nuclease-free water, and quantified using NanoDrop. RNA was stored at -80°C until RNA-seq library preparation. RNA-seq libraries were sequenced by Novogene, and raw paired-end FASTQ files were generated after base calling and demultiplexing.