Short read worker gut metagenome
Composed of 15 guts taken from workers. Homogenized in 3 ml of ice-cold PBS with a dounce homogenizer. Spun down at 500g These samples are from three single-drone-inseminated colonies: G86, O91, and W76. Fifteen nurses were collected per colony (determined by larval feeding behavior), stored in scintillation vials on ice. The mid, hind, and crops were collected  most dissected with crops intact, but many in O91 are missing the crop portion. All 15 guts per colonies were dissected directly into dounce homogenizers filled with 3ml chilled PBS, then homogenized. The pooled homogenate was split between three 1.5-ml tubes, which were pelleted at low speed to remove gut and pollen debris. The supernatant was then removed and spun at 5000g to pellet bacterial cells. The supernatant was then removed and the remaining pellets were flash-frozen in liquid nitrogen and stored at -80C until they were shipped in dry ice.