RNA-seq of holobiont Myxilla (Burtonanchora) lissostyla: 2021
Three adult individuals of the Antarctic marine sponge Myxilla (Burtonanchora) lissostyla obtained during Dec2021 from Chile Bay, Greenwich Island, South Shetlands, Antarctica were used. RNA was extracted from them using the MirVana miRNA kit with size fractionation, keeping the fraction >200 nt in the sample. A pool of these samples was created through RNA precipitation. The unified sample was purified, quantified and lyophilized for sequencing at Novogene Co., Ltda. For RNA-seq, the sample was enriched in mRNA using oligo-dT, and depleted of rRNA, prior to metatranscriptome library preparation and sequencing with an Illumina NovaSeq 6000, PE150.