Cell specific gene silencing by lipid-siRNA conjugates in mouse central nervous system barriers
Cell specific gene silencing by lipid-siRNA conjugates in mouse central nervous system barriers
Brain were extracted and dissociated into single cells following manufacturer instructions for the Miltenyi adult brain dissociation kit. Cells were counted on a hemocytometer and diluted to target 20,000 cells for encapsulation.  Cell were encapsulated using the PIPseq method, v4 chemistry. The PIPseq manufacturer's guide was then followed for nucleic acid extraction. The PIPseq manufacturer's guide was then followed for library preparation.