RNA-Seq of Meleagris gallopavo: mature LPAIV-infected symptomatic female uterovaginal junction
RNA quantity and quality assessment using the RiboGreen assay (Thermo Fisher Scientific, Waltham, MA, USA) and TapeStation 4200 (Agilent, Santa Clara, CA, USA), respectively. Samples with RNA integrity numbers (RIN) ranging from 8.0 to 9.4 were used for cDNA library construction. Indexed libraries were constructed with the TruSeq Stranded mRNA Sample Preparation Kit (Illumina, Inc., San Diego, CA, USA) and size-selected for approximately 200 bp inserts. Libraries were multiplexed and sequenced on an Illumina NovaSeq 6000 platform using a 150 paired-end flow cell to generate 51-bp paired-end reads.