GSM9670008: Control mouse 2, VDJ sequencing library; Mus musculus; RNA-Seq
Tumor tissue was dissociated enzymatically into single-cell suspension and CD45+ cells were isolated by FACS. Cells were stained with hashtag oligonucleotides (HTO1-HTO4, TotalSeq-C, BioLegend) for sample multiplexing prior to 10x Genomics loading. 10x Genomics Chromium 5' Gene Expression v3 chemistry with Feature Barcode (HTO) and B cell receptor (BCR) V(D)J enrichment libraries. For the VDJ library, sample index amplification PCR was run for 12 cycles rather than the suggested 8, due to low B cell numbers. Sequenced on Illumina NovaSeqX Plus with 50-bp paired-end reads.