GSM8992125: mES-E14-M1-FKBP-dTAG_REP2; Mus musculus; ATAC-seq
100,000 cells were harvested and centrifuged (500 g, 65 °C, 10 mins). The supernatant was removed, and cell pellet resuspended with 1 X lysis buffer (10 mM Tris HCL, pH 7.5, 10 nM NaCl, 3mM MgCl2, 0.1 % Igepal CA-630). Cells were centrifuged (500 g, 65 °C, 10 mins); the supernatant was removed and the nuclear pellet was incubated for 30 mins at 37 °C in 2 X tagmentation buffer (20 mM Tris-HCl pH 7.5, 10mM MgCl2, 20 % Dimethyl Formamide) with Tn5 transposase (Illumina, 20034197). Tagmented DNA was extracted with the Qiagen DNeasy blood and tissue kit. Tagmented DNA  waas amplified for sequencing using the NEBNext High-Fidelity 2× PCR Master Mix (New England Biolabs) and further purified with the QIAquick PCR Purification Kit (QIAGEN).