GSM9049671: M4-Young; Mus musculus; RNA-Seq
RNA was extracted according to the manufacturer's protocols for each kit. For RNA isolation, the optional on-column DNase digestion step (Qiagen, 79254) was included for all samples. RNA was extracted using either the AllPrep DNA/RNA Mini Kit (Qiagen, 80204; samples M1, M5, M7, M8, M9, M10, M13, and M18) or the RNeasy Mini Kit (Qiagen, 74104; samples M2, M3, M4, M6, M11, M12, M14, M15, M16, and M17). The Yale Center for Genome Analysis performed the following steps. RNA quality was estimated with A260/A280 and A260/A230 nanodrop ratios, and RNA integrity was evaluated using an Agilent Bioanalyzer gel. rRNA depletion, cDNA synthesis, and library preparation were done with the Kapa RNA HyperPrep Kit with RiboErase (KR1351).