GSM9422754: Oleic Acid Diet 1; Mus musculus; RNA-Seq
RNA was extracted using TRI reagent (MilliporeSigma cat#:T9424)according to the manufacturer's instruction. Genomic DNA was removed from 1μg total RNA with an RNeasy MinElute Cleanup Kit (QIAGEN cat#: 74204). To make RNA-seq libraries, mRNA was isolated from total RNA using a NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs cat#: E7490S). Libraries were constructred according to manufacturer's protocol using RNA-seq library preparation kit (New England Biolabs cat#: E7770S).