mRNA sequencing of control HUVECs (replicate 2)
This study aimed to investigate the molecular mechanism by which circ705 regulates endothelial function in the context of atherosclerosis. Human umbilical vein endothelial cells (HUVECs) were transfected with either a circ705 overexpression plasmid or a control plasmid. Total RNA was extracted 72 hours post-transfection, and mRNA libraries were constructed using the VAHTS Universal V6 RNA-seq Library Prep Kit. Transcriptome sequencing was performed on the Illumina NovaSeq 6000 platform to compare global gene expression profiles between control and circ705-overexpressing cells.