GSM9814711: Mouse cortex APOE4 astrocyte graft AD APP-70; Mus musculus; RNA-Seq
Total RNA was extracted from 5 mg of cortical brain tissue using the NYZ Total RNA Isolation Kit (NZYTech, MB13402). RNA quantity was assessed using the Qubit RNA HS Assay Kit (Thermo Fisher Scientific, Q32855) and RNA integrity was confirmed using the Agilent 2100 Bioanalyzer with Agilent RNA 6000 Pico Chips (Agilent Technologies, 5067-1513). All samples exhibited RIN values ranging from 7.8 to 9.6 (one sample RIN N/A, DV200=69%). Libraries were prepared using 10ng of total RNA input with the SMARTer Stranded Total RNA-seq Kit v3 - Pico Input Mammalian (Takara Bio, Cat. Nos. 634489-634492) and the SMARTer RNA Unique Dual Index Kit 24U (Takara Bio, Cat. No. 634451), following the manufacturer's user manual. Library quality and size distribution were verified on an Agilent 2100 Bioanalyzer using Agilent High Sensitivity DNA kit (Agilent Technologies, Cat. # 5067-4626). Library average size: 380 bp.