ABC
Three micrograms of RNA were used as input material for the RNA sample preparations. The mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in fragmentation buffer. Using these short fragments as templates, double-stranded cDNA was synthesized using random hexamer primers. Then, the double-stranded cDNA was subjected to end-repair and phosphorylation, and the A base was added to the 7 end. The Hieff NGS DNA Selection Beads were used for purification and fragment Selection. After purification and fragment selection, the products were amplified and enriched by PCR.