16S rRNA V3-V4 of eastern oyster 5 hemolymph notch-first notch-method replicate B
Paired-end sequencing (2  250 bp) of 16S rRNA gene amplicons spanning the V3V4 region (primers 341F/806R) was performed by Novogene (Tianjin Medical Laboratory) on an Illumina NovaSeq 6000 platform. An AATI Fragment Analyzer was used by Novogene prior to sequencing to evaluate the size and integrity of DNA fragments in the library.