shotgun metagenomics from calves' feces
DNA was extracted using QIAamp Fast DNA stool mini kit using 200 mg feces as starting material. DNA quality was checked using a Qubit Fluorometer. All libraries were prepared using the PCR-free Kapa Hyper Prep Kit (Roche), and sequenced on Illumina Novaseq 6000 S4 (2  150 bp) platform with an estimated output of 46 million reads per sample (average)