GSM8712334: HCT116_psy treated_replication1; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit (Lexogen, Austria) according to the manufacturer's instructions. Briefly, total RNA (1 µg) was used as the starting material, and poly(A)-tailed RNA was selectively enriched. First-strand cDNA synthesis was performed using an oligo-dT primer with an Illumina-compatible sequence. Second-strand synthesis was initiated by a random primer with an Illumina-compatible linker sequence. The resulting double-stranded library was purified using magnetic beads and amplified to add complete adapter sequences required for sequencing on the Illumina NextSeq 550 platform.