GSM9873641: SARS2_30dpi_1; Mus musculus; RNA-Seq
Mouse olfactory bulb samples were homogenized in TRIzol, and total RNA was isolated according to the manufacturer's protocol, followed by DNase treatment. Library preparation contains the following steps: 1. Poly-A mRNA capture and reverse transcription for cDNA synthesis: oligo-dT primer to capture just the mRNA (contains sample barcode, UMI, and Read 1 sequences). 2. Second strand synthesis and tagmentation: double stranded-cDNA is generated. Tagmentation creates fragments and incorporates Read 2 sequence. 3. Illumina library amplification: adds unique dual indices (UDI = i5, i7) and P5/P7 sequences.