RNA-seq expreiment of a striatin knockout mutant compared to wildtype Schyzophyllum commune
RNA-seq expreiment of a striatin knockout mutant compared to wildtype Schyzophyllum commune
Schizophyllum commune wild type and the ∆str1 mutant were inoculated in triplicate on CYM medium (Complex Yeast Medium; Schwalb and Miles, 1967) and incubated for 6 days. For RNA isolation and subsequent RNA sequencing, the fungal mycelium was harvested and ground to a fine powder under liquid nitrogen. Subsequently, 100 mg of frozen tissue was used for RNA extraction with the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany), according to the manufacturer's instructions. RNA samples were submitted to StarSEQ GmbH (Mainz, Germany) for transcriptome analysis. StarSEQ isolated mRNA from total RNA and prepared sequencing libraries using the NEBNext Ultra II Directional RNA Library Prep Kit. RNA integrity and concentration were verified using a Bioanalyzer and Qubit prior to library preparation.