Prokaryote microbiota (16S rRNA metabarcoding) of rhizosphere
DNA extraction was performed using 200 mg of rhizosphere soil and the DNeasy PowerSoil Pro Kit (Qiagen, Germany). Illumina NovaSeq 6000 sequencing with a paired-end approach (250 bp) was conducted targeting the V3-V4 region of the prokaryote 16S rRNA gene (primer pair 341F/805R).