RNA seq of human breast cancer cell line MDAMB231 with knockdown of splicing factors SRSF7 and SMNDC1
RNA seq of human breast cancer cell line MDAMB231 with knockdown of splicing factors SRSF7 and SMNDC1
A total of 30 million cells MDA-MB-231 cells were seeded in 6-well plates. The following day, cells were transfected with siKinasePool siRNAs targeting SRSF7, SMNDC1, or a mock control. Samples from three biological replicates were collected 72 hours after transfection.  Total RNA was isolated using the RNeasy plus mini kit (Qiagen). Samples from 3 biological replicates were used. RNA quality and quantity were measured on an Agilent-4200 Bioanalyzer. Stranded PolyA-selected libraries were prepared using the DNBseq platform.