Targeted Amplicon NGS-sequencing of Homo sapiens : HEK293T cells , transfected with TPC2 e8-SD sgRNA and evoBE4max plasmid, unsorted population, replicate 2, sequenced at the TPC2 locus
Samples were prepared for Amplicon-EZ-based NGS analysis (GeneWiz/Azenta Life Sciences). All locus-specific primers contained the 5 partial Illumina adapter sequences (Fwd: 5-ACACTCTTTCCCTACACGACGCTCTTCCGATCT-3, Rev: 5-GACTGGAGTTCAGACGTGTGCTCTTCCGATCT-3). Typically, samples corresponding to different target loci were pooled into a single sample. The pooled NGS data were demultiplexed by mapping to the respective reference sequences later. Here, demultiplexed samples are provided via CRISPResso2 in CRISPRessoPooled mode.