GSM8751873: C_5C_ZT10_M_MCC3804A269_S151; Mus musculus; RNA-Seq
A co-immunoprecipitation protocol was used as previously described in (Chandra et al., 2015; Becker-Krail et al., 2022; DePoy et al., 2024), with minor modifications. Briefly, tissue was homogenized and incubated overnight with an anti-hemagglutinin A (HA) antibody (Abcam, Cambridge, UK). HA-tagged ribosomes, conjugated to an anti-HA antibody, were then isolated using Dynabeads (Invitrogen, Waltham, MA, USA). RNA was extracted and purified using an EZNA RNA Isolation Kit (Omega Bio-Tek Total RNA Miniprep kit) with ENZA RNase-Free DNAase (Omega Bio-Tek, Norcross, GA, USA). cDNA synthesis: SMART-seq HT kit; Library prep: Nextera Flex