GSM8595727: Microglia/BAMs, IL-17A, Donor 6; Homo sapiens; RNA-Seq
Total RNA was extracted from whole cerebroids and isolated cells using RNeasy mini kit (Qiagen, 74104) according to the manufacturer's instruction in an RNase free environment. Residual genomic DNA was removed by on-column digestion with RNase-free DNase I (Qiagen, 79254). cDNA libraries were prepared with 50ng input at 14 cycles of amplification using Illumina Stranded mRNA Prep, Ligation kit (20040532)