GSM8679697: 4C_pCFTR_HepG2_rep2; Homo sapiens; OTHER
For 4C-seq, cells were fixed with formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was digested with Csp6I, then with DpnII. For 4C-seq, inverse PCRs are performed using the Expand™ Long Template PCR System (Roche) (as described in Krijger et al, 2020). Inverse PCR for each step were purified by using Nucleomag PCR beads, with a ratio 1. Sequencing were realized with Illumina, as single-end reads with 75-bp read length and generated FastQ files.