GSM9702042: ATAD3A-KI3; Mus musculus; RNA-Seq
Total RNA was extracted from mouse aortic tissues using TRIzol reagent according to the manufacturer's instructions. RNA quantity was measured by Qubit 2.0 Fluorometer, and RNA integrity was assessed by agarose gel electrophoresis and Qsep/Bioanalyzer. For eukaryotic samples, mRNA was enriched using oligo(dT) magnetic beads, fragmented, and reverse-transcribed to first-strand cDNA using random hexamer primers. Second-strand cDNA was synthesized, followed by end repair, A-tailing, adaptor ligation, size selection, and PCR amplification. Qualified libraries were sequenced on an Illumina platform.