NextSeq 2000 sequencing
Staphylococcus aureus biofilms were grown for 24 hours and subsequently treated with helium (control) or CAP for 2 minutes, or 700 ÂµM hydrogen peroxide. RNA was extracted 15 minutes after treatment for sequencing. Three biological replicates were performed for each condition.
Illumina Stranded Total RNA Prep with Ribo-Zero Plus kit (Illumina) with custom probes targeting S. aureus