GSM8787968: dCas9_rep2[RNA-Seq, 2]; Homo sapiens; RNA-Seq
72 hours post-transfection RNA was isolated using the RNeasy Plus mini kit (Qiagen). RNA integrity was first assessed using a Bioanalyzer 2200 (Agilent) RNA-seq libraries were constructed using the TruSeq Stranded Total RNA Gold (Illumina, RS-122-2303).The qualities of RNA-seq libraries were verified using the Tape Station D1000 assay (Tape Station 2200, Agilent Technologies) and the concentration of RNA-seq libraries were checked again using real time PCR (QuantStudio 6 Flex Real time PCR System, Applied Biosystem).RNA sequencing libraries were prepared with ERCC92 spike-in controls. Libraries were normalized and pooled prior to sequencing. Sequencing was performed using an Illumina Hiseq 3000 with paired end 75 base pair reads.