GSM9047727: Wildtype tumor CD45+ cells; Mus musculus; RNA-Seq
Tumor were harvested from 4 individual mice and pulled together to digest and process for signle cell suspension with a cocktail of Collagenase-II, Dispase-II, and Deoxyribonuclease I. CD45+ immune cells were FACS sorted using BD FACSAria II (BD Bioscience). Single-cell RNA-seq libraries were prepared using version 1 Chromium Single-cell 3′ Library, Gel Bead & Mutiplex Kit (10X Genomics) following to manufacturer's protocol. Sequencing was performed on Illumina NextSeq2500.