GSM9158043: cortex,P3_WT,rep2; Mustela putorius furo; RNA-Seq
P3 ferret pups were deeply anesthetized, and their brains were immediately harvested and immersed in ice-cold Hanks' Balanced Salt Solution (Gibco 14175-095). The dorsal cortices were dissected into small pieces and dissociated into a single-cell suspension using the Papain Cell Dissociation Kit, following the manufacturer's instructions (Miltenyi Biotec, catalog no. 130-092-628). Single-cell RNA sequencing (scRNA-Seq) libraries were prepared using the Chromium droplet-based sequencing platform (10X Genomics), in accordance with the manufacturer's guidelines (manual document part number: CG00052 Rev C). The cDNA libraries were purified, quantified using an Agilent 2100 Bioanalyzer, and sequenced on an Illumina HiSeq 4000.