GSM9670913: iPSC-derived microglia, GPNMB KO, PBS, biol rep 2; Homo sapiens; RNA-Seq
RNA was extracted using the RNeasy Plus Kit (Qiagen). RNA purity and concentration were measured using a NanoDrop spectrophotometer. DNase I treatment was performed prior to library preparation. RNA sequencing libraries were prepared using the Illumina TruSeq library preparation kit following the manufacturer's protocol. Single-end sequencing (118 cycles) was performed on an Illumina NextSeq 2000 instrument (P2 flow cell cartridge) at the Center for Host-Microbial Interactions (CHMI), University of Pennsylvania.