SMARTER amplified RNA-seq of Anomotaenia brevis: whole cysticercoids
In infected ants, we removed the cysticercoids, located in the haemolymph around the midgut, and placed them in a vial with 50 l Trizol. Samples were frozen on dry ice and subsequently transferred to -80C. RNA was extracted using the Qiagen RNeasy extraction kit. We sent the samples to Novogene (Cambridge, UK) for mRNA library preparation using a NEBNext Ultra RNA Library Prep Kit. For cestode samples, a SMARTer amplification step was performed to accommodate low RNA content. The libraries were sequenced on an Illumina Novaseq 6000 in paired-end 150 bp mode, yielding a minimum of 9 Gb per sample.