Whole genome sequencing (WGS) of Proteus mirabilis Indica clinical isolate from India.
A 1.5 ml bacterial culture was pelleted by centrifugation at 4000 rpm for 5min, and the supernatant was discarded. Phenol-chloroform method was followed for genomic DNA isolation. Quantitative & qualitative analysis was done by the fluorescent (Qubit fluorometer 4.0) & 0.8% agarose gel electrophoresis respectively. The libraries were prepared using the manufacturer instructions of QIAGEN QIAseq FX DNA Library Kit. These libraries were sequenced in a 2150bp paired-end run using the NovaSeq 6000 (Illumina) with v1.5 reagents (300 cycles).