GSM8819816: Round spermatids, rep1; Mus musculus; OTHER
Total RNA was isolated from germcells using TRIzol. Poly(A) RNAs were purified with oligoT beads. Direct RNA sequencing libraries were built according to the Oxford Nanopore Technologies protocol (Direct RNA Sequencing Kit- SQK-RNA002) with modifications to incorporate barcoded oligonucleotides for multiplexing. This analysis include 2 libraries, one for pachytene spermatocytes and one for round spermatids. The two libraries were prepared in parallel. Each library was built to multiplex 3 mRNA extracts (BC 1-3, here considered as replicate rep1-3), each representative of a pool of mRNAs from 3 independant mice.Libraries were run on Nanopore R9 chips with a GridIon instrument.