GSM9813474: Stingfl/fl Cdh5-Cre+ mouse liver, biological replicate 3; Mus musculus; RNA-Seq
Total RNA was extracted from liver tissues using standard RNA extraction protocols. Strand-specific RNA-seq libraries were constructed using the standard protocol for Illumina sequencing. mRNA was enriched using Oligo(dT) magnetic beads to capture polyA-tailed transcripts, fragmented, and reverse-transcribed into cDNA. After end repair, A-tailing, and adapter ligation, ~200 bp cDNA fragments were selected using AMPure XP beads, PCR-amplified, and sequenced.