RNA-Seq of analog astronauts: adult blood
Poly-A selected strand-specific mRNA library was prepared with Illumina Stranded mRNA Prep with UDP Set B index kit (Illumina, San Diego, California, USA). The sequencing was performed on the Illumina NextSeq 2000 platform, generating an average of 42 million paired-end 100 bp reads per sample. Quality control of raw sequencing reads was performed using FastQC (v0.11.9). Adapter sequences were then removed with Trim Galore (v0.11.9). Reads were aligned to the human reference genome (GRCh38.p14) using STAR (v2.7.10a) with the quantMode option enabled to generate raw read counts using HTSeq-count.