16S rRNA amplicon sequencing of coffee rhizosphere soil, replication 3
Genomic DNA extracted with Quick-DNA Magbead Plus Kit; 16S rRNA V1-V9 region amplified using KOD Multi & Epi (primers 27F-1492R); library prepared with Nanopore Native Barcoding Kit 96 V14 (SQK-NBD114.96); sequenced on Nanopore GridION