GSM9203157: GFP::rde-1, L4440 RNAi, input, rep1; Caenorhabditis elegans; ncRNA-Seq
Worms were washed from plates and rinsed with M9 3 times and flash frozed in liquid nitrogen. Total RNA from cell lysates or Argonaute co-immunoprecipitates was isolated using trizol/chloroform and isopropanol precipitation. Small RNA libraries were prepared according to the NEBNext Multiplex Small RNA kit or QIAseq miRNA Library Kit protocol, omitting the initial size selection step. PCR products corresponding to 16-30 nt small RNAs were size selected using 10% polyacrylamide gels.