Whole-genome sequencing of a weakly proteolytic strain of Bacillus velezensis
Long-read genomic DNA libraries were prepared by MicrobesNG (Birmingham, UK) using the Oxford Nanopore SQK-LSK109 ligation sequencing kit with Native Barcoding Expansion EXP-NBD104/114, using 400500 ng of high-molecular-weight genomic DNA. Barcoded samples were pooled and sequenced on a GridION device using FLO-MIN106 (R9.4.1) or FLO-MIN111 (R10.3) flow cells