GSM9558278: E_WT_TNF_Pol2_4h; Mus musculus; ChIP-Seq
Liver tissue was crosslinked with 1% formaldehyde for 10 min at 37 C with gentle shaking and quenched with 0.125 M glycine. Chromatin was isolated and sonicated to ~200-500 bp. Chromatin was immunoprecipitated with 2 ug control IgG (Santa Cruz Biotechnology), anti-RNA polymerase II (Active Motif), or anti-HNF4alpha (Abcam). Immunoprecipitated DNA was purified by phenol/chloroform/isoamyl alcohol extraction and ethanol precipitation. ChIP-seq libraries were prepared using TruSeq ChIP Sample Prep Kit (Illumina) and sequenced on Illumina HiSeq 2000 (single-end).