GSM8678703: PAO1 frame-shift deletion in the gacS, exponential growth phase, rep2; Pseudomonas aeruginosa PAO1; RNA-Seq
Total RNA was isolated from cells using hot phenol method. High quality total RNA was further processed by removing 23S and 16S rRNAs using the the Illumina Ribo-Zero Plus rRNA Depletion kit (Illumina, USA). Final concentrations and purity grades of the samples were determined using Bioanalyzer 2100 (Agilent, USA). Samples with the amount at least 0.5-1 µg were used for library construction and RNA sequencing. Libraries of depleted RNA were prepared using the Illumina TruSeq RNA LT v2 kit according to the manufacturer's instructions. RNA libraries were prepared for sequencing using Illumina protocols