GSM9131085: DJ_E_C_3; Oryza sativa; RNA-Seq
Total RNA was isolated from the root tips of rice plants using TRIzol reagent (Ambion, 15596018) according to the manufacturer's instructions. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection