GSM9289544: WT_Male_Tg+PDBu_inVitro_1; Mus musculus; RNA-Seq
RNA isolation with QIAGEN RNeasy Plus Mini kit (74134, QIAGEN) was performed according to the manufacturer's protocol. For adult microglia isolated via FACS,  RNA was isolated following manufacturer's instructions using QIAGEN RNeasy Micro kit (74004, QIAGEN). RNA quality and quantity were assessed using TapeStation 4200 RNA tapes (Agilent), and RNA-seq libraries were prepared from 100ng(microglia from in vitro assays) and 0.97ng (adult microglia isolated vis FACS)  of total RNA using the NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (NEB E7370L) and the SMARTer Stranded Total RNA-seq kit v2 (Takara Bio), respectively. Library QC was then performed using TapeStation 4200 DNA tapes (Agilent).