Mitochondrial Genome Sequencing of Lyreidus stenops: deep-sea specimen at 60-100m depth in the East China Sea
The specimen of Paraplax sp. was collected from the East China Sea at a water depth of 60-100 meters.Genomic DNA was extracted from muscle tissue using CTAB method. DNA quality and quantity were assessed using a NanoDrop spectrophotometer and Qubit fluorometer. A sequencing library was prepared using the TruSeq Library Construction Kit (Illumina, San Diego, CA, USA), with genomic DNA fragmented to an average size of 350 bp using a Covaris sonicator. Adapter ligation and PCR amplification were performed according to the manufacturers protocol. The library was sequenced on an Illumina NovaSeq 6000 instrument with 150 bp paired-end reads. Raw sequencing data were generated in FASTQ format and used for mitochondrial genome assembly.