GSM8626319: Tumor draining Lymh Nodes 1x8Gy 24h; Mus musculus; RNA-Seq
Tumors were harvested and treated with collagenase IV fro 30 minutes in 37C. Tumors were then smashed and passed through a 70um filter. Tumor cells were spun down and resuspended in TRIzol reagent. RNA extraction was done by the manufacture's guidance. A polyA approach was used with the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina in addition to ERCC RNA Spike-In Control Mixes at a 1:1000 ratio following manufacture instructions.