GSM8917382: PA-1 knockout replicate 2; Homo sapiens; miRNA-Seq
Total RNA from DGCR8 knockout PA-1 cells was extracted from a 60% confluent T75 cell flask  with the miRNeasy Mini Kit (Qiagen), following the protocol for Purification of Total RNA, Including Small RNAs, from Animal Cells was followed, including the additional on-column DNAse digestion. After extraction, ≥ 200 ng of total RNA was used for library sequencing with DNBseq SE50 by BGI Genomics. For small RNA-seq,  200 ng of total RNA was used for library sequencing with DNBseq SE50 by BGI Genomics.