GSM8627023: TransgelinKDCM2; Homo sapiens; RNA-Seq
Libraries were prepared using the NEBNext poly(A) mRNA magnetic isolation apparatus (New England Biolabs [NEB], Hitchin, UK) and the NEBNext Ultra RNA LibraryPrep Kit for Illumina (NEB). Gene expression analysis of SPF was performed by RNA sequence. Three SPFs, each established from separate cancer tissue samples, were transfected with sh luc or sh Transgelin#1. After introduction of the sh vector, RNA derived from stimulated and unstimulated cells was extracted by addition of cancer cell culture supernatant. The final analysis was performed using 3 (SPF type) × 2 (2 vectors) × 2 (with or without cancer cell culture supernatant added) for a total of 12 Samples. For Bioanalyzer (Agilent) quality control and next-generation sequencing analysis, total RNA samples were sent to Rhelixa Corporation.