RNA-seq of MNK3i + sgRNA treated (+/-) with doxycycline (2ug/ml) for 48hr and then stimulated with IL-1b and IL-23 (10ng/ml each) for 21hr.
Samples were sent in Trizol to GeneWiz/Azenta Life Sciences (South Plainfield, NJ, USA) for RNA extraction, DNAase treatment, ribosomal depletion, and 2x150 sequencing.