GSM9157919: single cell sequencing of human prepuse 1; Homo sapiens; RNA-Seq
The collected prepuce tissues were stored in the sCelLiveTM Tissue Preservation Solution (Singleron Biotechnologies, Nanjing, China) and then transported to the Singleron lab on ice as  soon as possible after animals.  The specimens were dissociated into single cell suspension with a Singleron PythoNTM Automated Tissue Dissociator (Singleron Biotechnologies) with sCelLiveTM Tissue Dissociation Mix (Singleron Biotechnologies),  based on the preset protocol for prepuce.  The sample was stained with trypan blue (Sigma, Shanghai, China) and microscopically evaluated for cell viability. Single-cell suspensions at 1 × 105  cells/mL in concentration in PBS (HyClone, Shanghai, China) were prepared and loaded onto microfluidic devices and scRNA-seq libraries  were constructed according to Singleron GEXSCOPE® protocol by GEXSCOPE® Single-Cell RNA Library Kit (Singleron Biotechnologies) and Singleron Matrix® Automated single-cell processing system (Singleron Biotechnologies).  Individual libraries were diluted to 4 ng/µL  and pooled for sequencing.  Pools were sequenced on Illumina Xplus with 150 bp paired end reads.