RNA-seq for U-2OS cells stably expressing polyamine sensor transgene
Total RNA was extracted with PureLink RNA Mini Kit (Invitrogen) per the manufacturers instruction. PolyA selection and RNA library construction were performed by Novogene with sequencing as 150x150 base paired-end libraries using an Illumina NovaSeq 6000 (Novogene), to a depth of ? 20M reads.