GSM8712687: C4-2 Enz-R 2; Homo sapiens; RNA-Seq
Total RNA from the cancer cells was extracted with TRIzol (Invitrogen). Briefly, 1ug purified mRNAs were used for RNA-Seq library generation with the NEBNext® Ultra™ RNA Library Prep Kit (# E7530, NEB, MA, USA) and were subjected to 150 bp paired-end sequencing on an Illumina HiSeq sequencer.