GSM8678063: M5_3; Mus musculus; RNA-Seq
total RNA was extracted following the manufacturer's instructions (TransZol Up Plus RNA Kit, Trans Gen Biotech). The RNA sample was accurately tested for RNA integrity by the Agilent 2100 bioanalyzer, and the qualified RNA samples were used to build a library using the Illumina TruSeq RNA Library Prep Kit v2. Simply put, the obtained mRNA is fragmented and used as a template to synthesize cDNA. PCR amplification after purification and screening of cDNA. A library was constructed using the purified PCR products.