RNA-Seq of Gallus gallus: IS/1494-like IBV infected kidney RNA
Total RNA was extracted from kidney tissues of SPF Leghorn chicks experimentally infected with the Iranian nephropathogenic IBV IS/1494-like strain using TRIzol reagent. Equal RNA amounts from birds within the infected group were pooled to generate one composite RNA sample. The library was prepared using the Illumina TruSeq Stranded mRNA Library Prep Kit with poly-A selection and sequenced as 150 bp paired-end reads on an Illumina HiSeq 4000 platform at BGI Shenzhen.