GSM9340260: C_01; Mus musculus; RNA-Seq
Total RNA from ventricular cardiac tissue of Vhl-KO and WT mice was extracted using the RNeasy Mini Kit (Qiagen) following standard protocols. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The kit for library preparation was the Novogene NGS RNA Library Prep Set (PT042).  The library was checked with Qubit 2.0, real-time PCR for quantification, and a bioanalyzer 2100 for size distribution detection. Sequencing was carried out on the Illumina platform NovaSeq6000 .