GSM8818277: CTL019 CAR T cells, Cas9 SpyFi Protein 4KO, D0, ND589, ATAC-seq; Homo sapiens; ATAC-seq
Cryopreserved vials of cells were thawed, washed, and treated with DNase I (Life Technologies) to eliminate genomic DNA contamination. Following cell lysis and cytosol removal, nuclei were treated with Tn5 transposase (Illumina, Cat. #20034197) for 30 minutes at 37 °C and purified using the MinElute PCR Purification Kit (Qiagen, Cat. #28004) to generate tagmented DNA. Tagmented DNA was barcoded with the Nextera Index Kit v2 (Illumina, Cat. #FC-131-2001) and amplified via PCR, followed by SPRI bead cleanup to obtain purified DNA libraries.