ONT of Acanthopagrus latusadult muscle
Genomic DNA was extracted from the muscle tissue of yellowfin seabream (Acanthopagrus latus). High-molecular-weight DNA fragments (>15 kb) were enriched and purified using magnetic beads. The fragmented DNA then underwent damage repair and end-repair processes. Following another round of purification, an 'A' tail was added to both ends of the DNA fragments. Finally, sequencing adapters from the SQK-LSK109 kit (Oxford Nanopore Technologies) were ligated to the fragments to complete the library construction.