GSM8625253: WJC_con(RNA); Homo sapiens; RNA-Seq
FACS sorted Live CD45+ cells from PDTSs of an ovarian cancer patient were resuspended in PBS. The viability was confirmed to be >90%. Single-cell suspensions were loaded on a 10x Genomics Chromium single-cell 3′ v3 chip to prepare the libraries according to the manufacturer's protocol. Sequencing was performed with Illumina NovaSeq 6000 according to the manufacturer's instructions (Illumina).