Nanopore-Seq of Weimaraner dog: adult female skeletal muscle; F8 gene region
The Ligation Sequencing Kit V14 (ONT) was used for sequencing and performed according to the manufacturer's protocol. FlowCells FAY11344, FAY11779, FAY34763 and FAY34559 were used for sequencing the DNA. The settings for sequencing on the GridION were: run limit 72h, pore scan freq. 1.5h, minimum read length 1000bp, read splitting ON, adaptive sampling ENRICH REFERENCE, basecalling HIGH-ACCURACY MODEL 400bps, Min Q score 9. The region 123938716 to 124754518 of the canine X chromosome (CFAX) was used as reference sequence (UU_Cfam_GSD_1.0, release 106).