16S rRNA Amplicon sequencing of human gut metagenome: longitudinal stool microbiota during a 12-week goat vs. cow milk intervention
Genomic DNA was extracted from human stool samples using the PureLink kit. The V3-V4 hypervariable region of the 16S rRNA gene was amplified using specific primers with barcodes. Libraries were constructed by end-repairing, A-tailing, and ligating with Illumina adapters. Sequencing was performed on the Illumina NovaSeq 6000 platform using a paired-end 250bp (PE250) strategy.