RNA-Seq of mus musculus: corneal epithelium, 2 months
The RNA-seq libraries were prepared using Illumina Stranded mRNA Prep Kit (#20040532, Illumina, San Diego, CA, USA) and IDT for Illumina RNA UD Indexes Set A (#20040553, Illumina, San Diego, CA, USA) according to manufacturer's instructions. The quality and quantity of the RNA-seq libraries were evaluated using Qubit 4 Fluorometer, NanoDrop One spectrophotometer, and 2100 Bioanalyzer Instrument. The RNA-seq libraries were multiplexed and sequenced on the Illumina Novaseq 6000 with a 2150 paired end (PE) configuration.