GSM9778191: Cardiomyocyte Cells, 4 days, 0 WNT inhibitor; Homo sapiens; RNA-Seq
Cells weredissociated with TrypLE, filtered through a 35 μm strainer (Thermo Fisher), resuspended in RPMI + B27 (noinsulin) with L-ascorbic acid and 10% DMSO,and frozen at −80 °C in a CoolCell LX container After thawing,cells were resuspended in fresh RPMI + B27 (no insulin) with L-ascorbic acid, kept on ice, stained with DAPI,and live singlets (DAPI−) were sorted on a CytoFLEX SRT into RPMI 1640 + B27 (noinsulin). Cell counts andviability were assessed by trypan blue on a Countess II. Approximately 6,000 cells per sample were loaded ontoa 10x Genomics Chromium Controller; libraries were prepared with the Chromium Single Cell 3′ v3 kit, pooled,and sequenced on a NextSeq 500