GSM8752902: allR in vitro 1x 2; Escherichia coli str. K-12 substr. MG1655; ChIP-Seq
TF-DNA complexes were fixed with formaldehyde and glycine.  DNA was sheared via sonication prior to TF-DNA binding.  Sheared DNA was immunoprecipitated (IP) using anti-6xHis mAb.  DNA was washed with IPP150 buffer and purified via ProteinaseK digestion and Qiaquick PCR purification kit (Qiagen - Cat. No. 28106). ChIP-DNA was converted into NGS libraries compatible with the Illumina platform using the NEBNext Ultra DNA (NEB Cat. No. E7465).