GSM8539453: hiPSC-EP, growth factor, rep 3; Homo sapiens; RNA-Seq
A total of 500,000 CD34+-hiPSC-EPs from both differentiation protocols were centrifuged twice and messenger RNA (mRNA) was isolated using an RNeasy Mini Kit (Qiagen) following the manufacturer's protocol. Each sample was diluted in nuclease-free water to a total volume of 25 µL at 25 ng/µL and submitted to the University of Texas at Austin Genomic Sequencing and Analysis Facility for 3'-Tag RNAseq (TagSeq) preparation. Library preparation was created by the Genomic Sequencing and Analysis Facility at the University of Texas at Austin.