SHAPE-MaP of yeast RNA: in vivo DMSO rep 2 part 3
Yeast was treated with DMSO, total RNA was extracted, followed by two rounds of mRNA enrichment using oligo (dT)25 magnetic beads. Then RNA was reverse transcribed with random nonamer primers in Mn2+ conditions, and dsDNA was made by non-directional second strand synthesis, followed by sequencing library preparation.