GSM8567621: GNP H3K4me3 ChIPseq replicate 1; Mus musculus; ChIP-Seq
GNPs and MB cells were purified using established percol fractionation protocol.   For crosslinking ChIPseq the single cell suspension was fixed in 1% foraldehyde for 10 min.  The chromatin was sheared using a Bioruptor.  ChIP was performed using Protein G agarose beads. For native ChIP GNPs or MB was incubated in MNase for 20 min and mononucleosomes wers isolated by centrifugation, ChIP was performed using Potein G agarose beads with an IgG/IgM linker.  Following elution DNA was purified by phenol chloroform extraction. ChIP-seq libraries were prepared using NEBNext ChIP-Seq Library Prep (New England Biolabs).  Sequencing was performed using HiSeq 2500 (Illumina Inc).