RNA-Seq of wild type
Sequencing libraries were generated by using the NEBNext UltraDirectional RNA Library Prep Kit for Illumina (NEB, USA). Finally, the products were purified (AMPure XP system), and library quality was assessed on the AgilentBioanalyzer 2100 system. The index-coded samples were clustered on a cBot Cluster Generation Systemusing TruSeq PE Cluster Kit v3-cBotHS (Illumina). After cluster generation, the library preparations were sequenced on an Illumina HiSeq platform, and paired-end reads were generated.