GSM9812780: ChIP-MNDA; Homo sapiens; ChIP-Seq
Cells were dissociated using TrypLE, harvested by centrifugation, and subsequently fixed and crosslinked in 1% formaldehyde at room temperature for 10 min. ChIP samples were prepared using a commercial Enzymatic ChIP Assay Kit. Chromatin fragmentation was achieved via MNase digestion at 37 °C for 20 min, followed by sonication to generate input chromatin. For MNDA immunoprecipitation, 15 μg input chromatin was incubated with 2 μg anti-MNDA antibody (Cat. No. 13576-1-AP, Proteintech, China) for target enrichment, while normal rabbit IgG was processed in parallel as the negative control. DNA from input, IgG control IP, and MNDA IP samples was finally purified with a DNA Clean & Concentrator kit. Sequencing libraries were constructed from the three purified DNA samples with a DNA Library Prep Kit for Illumina. After size separation via 2% agarose gel electrophoresis, target DNA fragments were recovered by gel extraction to generate final ChIP-seq libraries.