Tn-Seq alfalfa seed control replicate 1
Transposon insertion sequencing (Tn-Seq) of an EZ-Tn5 mutant library of Salmonella enterica serovar Typhimurium strain 4/74 recovered from alfalfa seed and sprout colonization experiments. Libraries were prepared using the Fasteris Tn-Seq V6 protocol and sequenced on an Illumina NovaSeq 6000 platform. Sequencing reads originate within the transposon and extend into adjacent genomic DNA to identify transposon insertion sites and quantify mutant fitness.