Mck_HighCO2
Total RNA was isolated from approximately 50 mg of maize leaf tissue using the TRIzol Reagent (Thermo Fisher Scientific, Waltham, MA, USA), followed by DNaseI treatment and purification using the RNA Clean and Concentrator-5 kit (Zymo Research, Irvine, CA, USA). RNA samples were quantified using the Qubit 4.0 Fluorometer (Invitrogen), and RNA integrity was evaluated using the Agilent 2100 Bioanalyzer (Iowa State University DNA sequencing Facility, Ames, Iowa). All RNA samples used for sequencing had an RQN (RNA Quality Number) equal to or > 6.5. Library preparation was performed using the QuantSeq 3 mRNA-Seq Library Prep Kits (Lexogen, USA), and samples were sequenced using the Illumina Novaseq 6000 System (Iowa State University DNA Facility, Ames, Iowa).