V4 16S sequencing of Periplaneta americana: adult hindgut
DNA was extracted from hindgut samples homogenized in 1x PBS using the Omega BioTek EZNA bacterial DNA kit. Custom barcoded libraries of the 16S rRNA gene were generated using a two-step PCR protocol with 515F and 806R primers. PCR products were verified by gel electrophoresis and purified with the Omega BioTek EZNA Cycle pure kit.