Mouse liver RNA-seq: HFD+SYN biological replicate 6
Non-strand-specific paired-end 150-bp bulk RNA sequencing of total RNA isolated from liver tissue of a male C57BL/6J mouse fed a 45% kcal high-fat diet and treated with an ellagic acid-Gordonibacter urolithinfaciens synbiotic. Libraries were constructed from fragmented mRNA using random oligonucleotide priming and the VAHTS Universal V8 RNA-seq Library Prep Kit for Illumina (NR605), with approximately 250-300 bp cDNA fragment selection, and sequenced on an Illumina NovaSeq X Plus.