GSM9777813: TXMS subcutaneous xenograft mouse tumors from TXMS1 cells injection, biol rep 1; Sus scrofa; RNA-Seq
RNA extraction was performed using Direct-zol RNA Miniprep Plus kit (Zymo). RNAseq libraries were prepared with the Kapa Hyper Stranded mRNA library kit (Roche). Read 1 aligns to the ANTISENSE strand and Read 2 aligns to the SENSE strand. The libraries were pooled; quantitated by qPCR and sequenced on one 10B lane for 151 cycles from both ends of the fragments on a NovaSeq X Plus with V1.0 sequencing kits. Fastq files were generated and demultiplexed with the bcl2fastq v2.20 Conversion Software (Illumina). The quality-scores line in fastq files use an ASCII offset of 33 known as Sanger scores