GSM9203080: Mouse_P1_oocyte_Ctrl_3; Mus musculus; RNA-Seq
Five to ten putative primordial oocytes per group were collected by a mouth pipette into one tube for mRNA amplification which was performed following the manufacturer's instructions (Vazyme, N712-03) with 18 amplification cycles. Library preparation followed the methods in (Chen et al., 2023). Briefly, after test the concentration by Qubit Flex Fluorometer (Thermo Fisher Scientific) and fragment size by Agilent Bioanalyzer 2100, TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme, TD503) was used to prepare the library.