ATAC-seq of Sus scrofa: 3D4/22 porcine alveolar macrophage cells infected with Mycoplasma hyopneumoniae
ATAC-seq library prepared from 3D4/21 porcine alveolar macrophage cells (Control and Mycoplasma hyopneumoniae-infected). Nuclear extraction was performed following the protocol described in 'ATAC-seq: A Method for Assaying Chromatin Accessibility Genome-Wide' for cell samples. Transposition was performed using 50,000 nuclei with Tn5 transposase (Illumina), and DNA fragments were purified using the MinElute PCR Purification Kit (Qiagen). PCR amplification was carried out with NEBNext High-Fidelity PCR Master Mix (New England Biolabs), followed by purification using MinElute PCR Purification Kit. Libraries were sequenced using an Illumina NovaSeq 6000 platform with paired-end 151 bp reads.