GSM9814126: ICRH122 (Baseline) RNA; Homo sapiens; RNA-Seq
Human pancreatic islets were isolated in the Human Islet Core at the University of Pennsylvania following the requirements of the Clinical Islet Transplantation consortium procedure. Islets were grown in CIT culture medium in a humidified incubator with 5% CO2 at 37 C. For perturbation samples (HPAP059, ICRH134, ICRH135), islets were treated with cytokine cocktail or infected with CVB4, alongside a mock control. Single-cell suspensions and single-nucleus preparations were generated for scRNA-seq and snATAC-seq, respectively, following the 10X Genomics sample-preparation protocols. scRNA-seq libraries were prepared using the 10X Chromium Single Cell 3' Library Kit (v3 chemistry). snATAC-seq libraries were prepared using the 10X Chromium Single Cell ATAC Library Kit (v1 chemistry). Libraries were sequenced on an Illumina NovaSeq 6000 at the Weill Cornell Medicine genomics resources core facility. The three HPAP059 snATAC-seq libraries were sequenced on an Illumina NovaSeq SP at Novogene.