recA gene sequence of Burkholderia cenocepacia
The recA gene was sequenced from Burkholderia cenocepacia clinical isolates for species-level identification and phylogenetic analysis. Genomic DNA was extracted from clinical bacterial isolates then the recA gene was amplified using BCR1 (5-TGACCGCCGAGAAGAGCAA-3) as a forward primer and BCR2 (5-CTCTTCTTCGTCCATCGCCTC-3) as a reverse primer with an expected product size of 1043 bp. The PCR products were treated with the DNA Clean and Concentrator-25 purification kit (ZYMO RESEARCH, USA). Sequencing was carried out using the API 3730*1 DNA sequencer. Chromatograms were manually inspected, and low-quality bases were trimmed using the FinchTV tool. Raw trace files are included in this submission.