RNA-seq of TD-MSCs
Total RNA was extracted from rat MSCs (AD-MSCs, BM-MSCs, TD-MSCs). Libraries were prepared using the TruSeq Stranded Total RNA kit and sequenced on an Illumina platform (paired-end 2100 bp). Data were aligned to the Rattus norvegicus (rn6) genome using HISAT2, and gene expression was quantified with StringTie.