GSM9230546: SD rat1, Right  Ear skin,OE-Fgf7; Rattus norvegicus; RNA-Seq
RNA was harvested using Rneasy mini plus kit (Qiagen). 1 ug of total RNA w as used for the construction of sequencing libraries. Total RNA was extracted from samples using standard methods, and RNA integrity was assessed with Agilent Bioanalyzer. Poly(A)+ mRNA was enriched using oligo(dT) beads (or rRNA was depleted, depending on experiment). The purified RNA was fragmented and reverse-transcribed to cDNA. After end repair, A-tailing, and adaptor ligation, cDNA fragments were PCR-amplified to generate sequencing libraries. The final libraries were quality-checked and sequenced on the Illumina NovaSeq X Plus platform.