ETNP_2018
515F (5-AATGATACGGCGACCACCGAGATCTACAC <i5> TATGGTAATT GT GTGCCAGCMGCCGCGGTAA-3) and 806R (5-CAAGCAGAAGACGGCATACGAGAT <i7> AGTCAGTCAG CC GGACTACHVGGGTWTCTAAT-3). Amplicon PCR reactions contained 1 l of template DNA, 2 l of 10 M forward primer, 2 l of 10 M reverse primer, and 17 l of AccuPrime Pfx SuperMix (Thermo Fisher Scientific by Invitrogen). The thermal cycle settings started at 95C for 2 min, followed by 30 cycles of 95C for 20 s, 55C for 15 s, and 72C for 5 min, and final elongation at 72C for 10 min.