GSM9523174: FPCRC; Mus musculus; RNA-Seq
A moderate amount of tumor was placed in ISOGEN (NIPPON GENE), crushed with a mixer, and RNA was extracted according to ISOGEN protocol. Ribosomal RNA was depleted using Ribo-Zero Plus rRNA Depletion Kit (Illumina) and the library was prepared using a SMARTer Stranded Total RNA-Seq Kit v2- Pico Input Mammalian (TAKARA BIO) .