GSM9050384: IP_ZT2_SD_4_R1_cortex; Mus musculus; ChIP-Seq
Samples rapidly dissected, flash-frozen in liquid nitrogen and stored at −140°C. Enrichment for BMAL1-bound chromatin regions was accessed using the previously described ChIPmentation protocol (Schmidl, C. Nat Methods 2015) with anti-BMAL1 antibody (ABCAM, AB3350). Libraries were prepared from purified DNA fragments using Nextera dual index primers. For practical reasons, input samples were sequenced at one third of the depth and the result pooled together as an average for each condition and ZT.