RNAseq of Bunonema sp. JU3391: mixed stage and sex population
10L (approximately 10mg) of live worm pellet was flash-frozen on liquid nitrogen and tissues were disrupted using the Cell Crusher Mini system (https://cellcrusher.com/mini-tissue-pulverizer-3/). RNA extractions were done with the Qiagen RNeasy Mini Kit (cat. no. 74104) following manufacturer protocol. Samples were sent on dry ice to GENEWIZ for standard RNA sequencing