GSM8754087: CUT&Tag of RFC3  in Early_S cells rep1; Homo sapiens; OTHER
RNA sequencing libraries were prepared using the phenol/chloroform extraction method. Single-cell isolation was performed with the 10x Genomics Chromium Single Cell Solution platform. For CUT&Tag experiments, cell capture was achieved using ConA-coated magnetic beads for cell enrichment. RNA libraries were prepared using the VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina® according to standard Illumina instructions. And CUT&Tag libraries were prepared according to the protocol established by Steven Henikoff's laboratory.scRNA-seq libraries were prepared following the manufacturer's protocol (10x Genomics).