GSM8712465: KK_2; Mus musculus; RNA-Seq
The total RNA was isolated from testicular enriched fractions of pachytene spermatocytes using Trizol (Invitrogen) following manufacturers' protocol.1 ug of total RNA was used for the construction of RNA sequencing libraries.25 ng CB RNA was used for the construction of sequencing librarie.200 ng total RNA was used for the construction of small RNA sequencing libraries. RNA libraries for RNA-seq were generated using the NEBNext® UltraTM RNA Library Prep Kit for Illumina®  in accordance with the manufacturer's recommendations.