HiC of mexican lime leaf tissue
Nuclei were isolated from fresh-frozen plant tissue samples using the CelLyticTM Plant Nuclei Extraction Kit (Sigma). Hi-C data was generated utilizing the High Coverage HiC kit from Arima, adhering to the manufacturer's instructions. In brief, chromatin was cross-linked with formaldehyde and digested with a proprietary cocktail of restriction enzymes. Following this step, the 5'-overhangs were filled in and labeled with a biotinylated nucleotide and spatially proximal digested ends of DNA were ligated. The ligated DNA was then purified and sheared to 550600 bp by sonication and the biotinylated fragments were selectively enriched. Subsequently, the enriched proximally-ligated DNA fragments were subjected to library preparation using KAPA HyperPlus Kits (Roche), including end-repair, dA-tailing, adapter ligation and PCR amplification. Finally, the Hi-C library was sequenced on the Illumina Novaseq X plus platform with 150 bp paired-end reads.