GSM9289771: Primary rat hepatocyte, DMSO, 0.5uM, rep1; Rattus norvegicus; RNA-Seq
RNA extraction was performed by using the TempO-seq platform (Biospyder) according to manufacturer's instructions. Transcriptomic profiling was conducted using the BioSpyder TempO-Seq Human Whole Transcriptome targeted gene panel (version 2.0), which interrogates over 20,000 protein-coding genes according to the manufacturer's protocol (BioSpyder Technologies, Carlsbad, CA, USA).  In TempO-Seq (Harril et al., 2021; Mav et al., 2018), each Detector Oligo (DO) contains a sequence complementary to an mRNA target and a universal primer binding site shared across all genes. DOs anneal to the RNA template at specific target sites and can be ligated together. Ligated DOs are then amplified using a single-plex PCR with a universal primer pair, incorporating both Illumina adaptors and sample-specific barcodes. This dual-index barcoding enables deconvolution of sample reads through standard Illumina software, producing a FASTQ file for each sample. For transcriptomic analysis, three biological replicates were profiled per concentration, per chemical, in addition to vehicle and positive control groups.