Metagenomic sequencing of fecal sample from thyroid antibody-positive infertile patient
Whole-genome shotgun metagenomic sequencing of fecal DNA. Genomic DNA was fragmented to ~350bp using Covaris ultrasonication. Library preparation included end repair, A-tailing, adapter ligation, purification, and PCR amplification. Quality control was performed using AATI for fragment size and Q-PCR for quantification. PE150 sequencing was conducted on Illumina NovaSeq platform.