Twist viral metagenomic sequencing of homo sapiens: adult male plasma
cDNA generated with Random Primer 6 and the ProtoScript II First Strand cDNA synthesis kit and NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module. cDNA processed with Twist Total Nucleic Acids Library Preparation EF Kit 2.0 and Twist UDI Primers. Indexed pools were hybridized at 70C for 16 hours with the Twist Comprehensive Viral Research Panel and amplified with KAPA HiFi HotStart ReadyMix.