GSM9049497: DMSO- replicate3; Danio rerio; RNA-Seq
At each time point, embryos were collected in RNA zol, homogenized and RNA extraction was conducted using a Directzol RNA Miniprep Plus kit (Zymo). RNA quality and quantity was validated using an Agilent Tapestation and Nanodrop. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library. For the non-directional library, it was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification(