RNA Sequencing of Tumor Tissues from Patients with Breast Cancer
Total RNA was extracted from clinical tissue samples using the NucleoSpin RNA Kit (Macherey-Nagel, Duren, Germany) according to the manufacturers instructions.Barcoded library were generated using the total RNA Library Preparation Kit (Illumina, San Diego, CA, USA). The libraries were sequenced on the Illumina NovaSeq 6000 instrument, using 2  151-bp paired-end sequencing flow cells, following the manufacturers instructions.