snRNAseq_L_vannamei-Sublib_A1
We set up a pathogen challenge comprising of 32 adult whiteleg shrimp infected by either feed or injection of WSSV. Mid to late-stage infected individuals were sacrificed and their lymphoid organs were collected. We used TST-based nuclei isolation and Evercode Nuclei Fixation v2 kit from Parse Biosciences to prepare our samples (~5000 nuclei/sample). Samples were sequenced by Novogene using Illumina NovaSeq X Plus, PE150 sequencing on the 25B flow cell (2% PhiX). Approximately 1.5 billion reads across 6 samples were obtained, for an estimated ratio of 50,000 reads per cell.