Expression profiling of platelets from young (3 months) homozygous Jak2 R1063H mice and wild-type controls
Expression profiling of platelets from young (3 months) homozygous Jak2 R1063H mice and wild-type controls
For platelets (PLT) isolation, whole blood was collected to acid-citrate-dextrose tubes supplemented with 3 uM prostaglandin E1 (PGE), and PLT-rich plasma was collected by centrifugation. Pelleted PLTs were subjected to red cell lysis and resuspended in the presence of anti-CD45 beads (Milteney Biotec) to remove residual leukocytes. Total RNA was isolated using RNeasy Micro Kit (Qiagen) as described in the manufacturer's protocol. Libraries were prepared with a SMARTer® Stranded Total RNA-Seq – Pico Input Mammalian library preparation kit v2 (Takara).