Region Capture Micro-C of K562
DNA was crosslinked and digested with micrococcal nuclease (MNase), followed by biotin labelling of digested ends, ligation, reverse crosslinking and streptavidin pulldown of ligated ends. DNA was size-selected for dinucleosomal size bands with gel extraction, then adapters were ligated and libraries were PCR amplified for sequencing. Finally, regions of interest were captured with biotinylated oligos following the TWIST capture enrichment protocol.