phoD gene sequences of microorganisms from coastal nearshore waters
High-throughput sequencing of the purified phoD gene amplicons was performed on the Illumina NovaSeq 6000 platform (Illumina, San Diego, CA, USA) by Magigene Biotechnology Co., Ltd. (Guangzhou, China), following the standard Illumina sequencing protocol to generate high-quality sequence reads for subsequent bioinformatic analysis.