GSM8819558: Zebrafish Larvae_Control IP-Nrf2; Danio rerio; ChIP-Seq
Whole larvae were crosslinked with 1% formaldehyde and quenched with glycine. Further, the chromatin was ultrasonicated under optimal conditions. Around 300-500 bp chromatin fragments were used for immunoprecipitation. Library was constructed for immunoprecipitated DNA using NEBNext Ultra DNA Library Preparation Kit and the sequencing was performed with the Illumina NovaSeq 6000/ HiSeq X (2x150, paired-end).