GSM8436665: HCT116, hypoxia 4 hours, 75nM Onvansertib, rep1; Homo sapiens; RNA-Seq
Cells were lysed in 700 µL of Trizol LS Reagent and total RNA isolated using the PureLinkTM RNA Mini Kit. RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina and paired-end sequenced (2 x 150 bp) on an Illumina NovaSeq.