GSM8752112: BEL-A_expansion_differentiation_Rhapsody_Pilot_3; Homo sapiens; RNA-Seq
Pooled cells from both harvests were labelled with oligonucleotide-conjugated antibodies for surface markers ('AbSeq') CD71, CD36, CD45, CD44, CD34, CD235a and CD49d. About 25,000 cells were captured into single cartridge well on a BD Rhapsody Express Instrument and each provided with a bead containing oligo nucleotide tags for the cell, unique molecular identifiers and a poly-T tail. All steps were carried out according to the manufacturer's instructions at https://www.bdbiosciences.com/content/dam/bdb/marketing-documents/BD-Rhapsody-Single-Cell-Analysis-System-Instrument.pdf Subsequent cDNA libraries were amplified and sequenced with Illumina NovaSeq6000/PE150. Reads were mapped to genes with the Rhapsody Targeted Analysis Pipeline (SevenBridges) and transcriptomes analysed with SeqGeq™/v1.7 (BD Biosciences).