E2F4-p53 Re-ChIP seq in HCT116 p21-/- cells & HCT116 p53-/- TAD mutant cells
E2F4-p53 Re-ChIP seq in HCT116 p21-/- cells & HCT116 p53-/- TAD mutant cells
Cells were harvested from 6 well-plate, followed by trypsinization, crosslinking and fixaton. Cells were grown under asynchronous condition in McCoy media HCT116 p21-/- cell were grown in 6 well plate, without any treatment.  HCT116 p53-/- p53 TAD mutant grown in 6 well in presence of Doxycycline. Cells were lysed using a nuclear lysis buffer. Followed by sonication of genomic DNA. 1ng of DNA used for library preparation. The Re-ChIP DNA libraries were constructed using the Kapa Hyperprep Kit with sample purification beads according to the manufacturerâ€™s instructions.