RNA-Seq of Rocahepevirus ratti in Rattus norvegicus
Wild rats were trapped in different sites of Berlin. Rats were dissected and RNA was extracted from the rat's liver. RAt HEV positive samples were used for NGS analysis. Libraries were generated using the KAPA RNA HyperPrep Kit or Illumina platforms (Roche Diagnostics, Mannheim, Germany). Target enrichment was performed using the myBaits NGS target enrichment system (Arbor Bioscience, Ann Arbor, MI, USA obtained from BioCat, Heidelberg, Germany) with the HEV myBaits set designed by Victor Max Corman (Institute of Virology, Charit Universittsmedizin, Berlin).