2bRAD-M sequencing of  homo sapiens: adults hair root
The objective of this study was to explore genetic variation and population structure in human hair root samples using 2bRAD-M seq, a reduced-representation genome sequencing technique. Genomic DNA was isolated from hair root samples using a standardized DNA extraction protocol. The 2bRAD libraries were prepared by digesting the genomic DNA with the Type IIB restriction enzyme BsaXI, followed by adapter ligation and PCR amplification. Sequencing was carried out on the Illumina NovaSeq 6000 platform, generating 150 bp paired-end reads. The resulting sequencing data were analyzed using the 2bRAD-M pipeline for SNP calling and subsequent population genetic analysis.