exDNA from River site upstream of WWTP discharge
Library preparation was performed using a KAPA Hyper Prep kit with PCR (Roche, Switzerland) per the manufacturers recommendations. Library quality and quantity were assessed with Qubit 2.0 DNA HS Assay (ThermoFisher, USA), Tapestation High Sensitivity D1000 Assay (Agilent Technologies, USA), and QuantStudio 5 System (Applied Biosystems, USA). Illumina 8-nt dual-indices (Illumina, USA) were used for indexing and barcoding. Equimolar pooling of libraries was performed based on QC values prior to sequencing.