GSM8450609: V65_dMir290e_r1; Mus musculus; RNA-Seq
RNA samples were isolated using AllPrep DNA/RNA Mini Kit (QIAGEN 80204). RNA libraries were prepared using KAPA RNA HyperPrep Kit with RiboErase (HRM) (Roche KK8651). Briefly, rRNA were depleted by hybridization of complementary DNA oligonucleotides and treatment with RNase and DNase. RNA samples were fragmented, and double strand cDNA were generated by two steps of cDNA synthesis. Illumina TruSeq DNA UD Indexes (IDT 20040870) were used to make libraries. Libraries were amplified using KAPA HiFi HotStart ReadyMix (2X) and Library Amplification Primer Mix (10X), followed by Qubit dsDNA quantification (Invitrogen Q32853), and 2100 BioAnalyzer D1000 kit (Agilent 50671504) and Illumina MiSeq Nano v2 QC.