RNA-Seq of Gallus gallus: uninfected control kidney RNA
Total RNA was extracted from kidney tissues of uninfected control SPF Leghorn chicks using TRIzol reagent. Equal RNA amounts from birds within the control group were pooled to generate one composite RNA sample. The library was prepared using the Illumina TruSeq Stranded mRNA Library Prep Kit with poly-A selection and sequenced as 150 bp paired-end reads on an Illumina HiSeq 4000 platform at BGI Shenzhen.