16s Amplicon sequencing of Mus musculus: fecal sample
The library was prepared by 2  Phanta Max Master Mix (VAZYME, China) polymerase, and the V3V4/V4/ITS1/ITS2 variable region of 16S/ITS rDNA of bacteria/fungi was amplified by forward and reverse PCR degenerate primers F and R (see remarks for primer sequences)