GSM9131738: AAD; Mus musculus; RNA-Seq
Use scissors to divide the vascular tissue into small pieces. Wash the tissue with PBS three times. Add digestive enzymes to the test tube to obtain a single-cell suspension. Cell viability should be above 80% and have good monodispersity with low impurity content. Generally, the cell concentration should be controlled above 205 cells/uL, and the number of cells should be more than 100,000. By utilizing the different nucleic acid sequence sizes contained in the large and small magnetic beads, separate libraries are independently constructed:Large magnetic bead library: Release the cDNA carrying Cell Barcode and UMI, after reverse transcription and amplification, add sequencing adapters to construct a transcriptome library; Small magnetic bead library: Extract the Oligo index information, generate an index library, which is used to locate the droplet position and verify the uniqueness of the tags.