GSM8595802: MA-VSMCs, calcium medium, LV-Vector, rep3; Mus musculus; RNA-Seq
RNA was purified using RNeasy Mini Kits (QIAGEN, 74104) from MA-VSMCs after treatment with calcification medium or without it, combined with or without αKlotho lentiviral vector transfection (each in triplicate). RNA was quantified using an Agilent 2100 Bioanalyzer Instrument (Agilent Technologies, USA). RNA sequencing libraries were prepared and sequenced at the Illumina facility.