GSM9701630: CD8+ T Cells, sham or 3Gy, infected 4dpi or 7dpi; Mus musculus; RNA-Seq
Cells were recovered in phenol red free RPMI1640 complete medium (1% L-Glutamine, 5% HEPES, 1% NEAA, 1% Sodium Pyruvate, 50 μM 2-ME, and 0.1% Gentamicin) with 10% fetal bovine serum, and then subjected to red blood cell lysis in ACK lysis buffer. The reaction was neutralized with RPMI1640 complete medium and washed with RPMI1640 complete medium twice. Cell pellets were resuspended in RPMI1640 complete medium for T cell purification. Total CD8+ T cell purifications were performed using the MojoSort Mouse CD8 T Cell Isolation Kit (BioLegend) following manufacturer's guidelines. Library construction was performed according to the manufacturers instructions (single cell Chromium Fixed RNA Profiling Reagent Kit for Multiplexed Samples protocol, 10X Genomics)