GSM9423176: 1822, NaDCA0.1%, rep2; Candida tropicalis; RNA-Seq
Ribonucleic acid (RNA) was extracted via ReliaPrepTM RNA Miniprep Systems (Promega, US). In addition to vortexing with 0.3–0.4 mm zirconia beads (As One, Japan) for two and a half minutes after mixing with isopropanol, the manufacturer's protocol was followed. The quality of the extracted RNA was measured with a NanoDrop One C (Thermo Fisher, US) and 5200 Fragment Analyzer (Agilent Technologies, US). Samples with an RNA integrity number (RIN) > 7.0 were selected for library preparation. RNA-seq libraries were prepared using the MGIEasy Fast RNA Library Prep Set (MGI Tech, China) according to the manufacturer's protocol. ﻿The quality of the library was checked with an Agilent 2100 bioanalyzer and a high-sensitivity DNA kit (Agilent Technologies).