GSM9153455: 2689_V2 (MAIT); Homo sapiens; RNA-Seq
RNA from sorted MAIT cell samples was extracted as previously described (Barnitz et al., Methods Mol Biol, 2013). RNA quantity and integrity was assessed using a Qubit 2.0 Fluorometer (Thermo Fisher Scientific) and Agilent TapeStation and submitted to the Oxford Genomics Centre (Centre for Human Genetics, University of Oxford) for library generation and sequencing (Illumina NovaSeq 6000, 150 base pair paired-end reads). NEBNext Single Cell/Low Input RNA Library Prep Kit (New England Biolabs, #E6240).