GSM9203552: HCC1806 cells, 2D monolayers, replicate 1; Homo sapiens; RNA-Seq
Total RNA was isolated using the RNeasy Mini kit (Qiagen), according to the manufacturer's instructions. RNA concentration and integrity were assessed using the QuBit Fluorometer and 4200 TapeStation System, respectively. Human mRNA-seq was performed at Novogene using the NovaSeq X Plus Series to obtain mRNA libraries (polyA enrichment) containing 150 bp pair-end reads with at least 85% of reads having a quality score of Q30 or higher.