Didymella segeticola treated with DMSO for 36 hours
The mycelium plug (diameter 4 mm) at the edge of CGMCC3.20152 colony 7d was transferred into PDB medium, and inoculated in an incubator at 25 for 36h. The RNA of the total sample was isolated and purified by TRIzol (thermofisher, 15596018) according to the operation scheme provided by the manufacturer. Then the quantity and purity of total RNA was controlled by NanoDrop ND-1000 (NanoDrop, Wilmington, DE, USA) and the integrity of RNA was detected by Bioanalyzer 2100 (Agilent, CA, USA). The concentration was >50ng/L, RIN value >7.0, and total RNA>4g.