GSM9814528: scRNA-seq_4NQO_OSCC_Tongue_Rep2; Mus musculus; RNA-Seq
Tongue tissues were placed in ice-cold PBS with 1% penicillin-streptomycin, dissected, and cut into ~1 mm³ pieces. Enzymatic digestion was performed using 0.25% trypsin and 0.1% collagenase type I at 37°C for 30 minutes with agitation. The reaction was stopped with fetal bovine serum (FBS), and the suspension was filtered through a 70 µm strainer. Only samples with >90% viability (Trypan Blue) proceeded to sequencing. Single-cell RNA sequencing libraries were constructed using the 10x Genomics Chromium platform. Approximately 10,000 cells per sample were loaded. Following cDNA synthesis and library preparation, sequencing was performed on an Illumina NovaSeq 6000 platform.