CUT&RUN of AICDA_dTAGV-1-3
CUT&RUN was performed using the CUTANA ChIC/CUT&RUN Kit (Epicypher) on AICDA-degron cells treated with 0.5 M NEG or dTAGV-1 for 4 hours followed by 200 ng/ml dox for 2 days. Nuclei were extracted, bound to concanavalin A-coated beads, and incubated with specific antibodies overnight. MNase digestion was performed, and DNA was purified using SPRIselect beads.