GSM8625408: mice bone marrow, RBD replicate 1, scRNAseq; Mus musculus; RNA-Seq
Bone marrow cells were isolated from tibia and femur bones following removal of surrounding muscle. Joints were cut using a scalpel and the exposed BM was flushed from the ends of bones using a 25-gauge needle and a 3 ml syringe filled with 2% FBS PBS. Clumps were gently disaggregated using a needle-less syringe and passed through a 70 μm cell strainer. The cell suspension was pelleted by centrifugation at 500 g for 5 min at room temperature. For 10X, single cells were processed with chromium next GEM single cell 3' HT reagent kits v3.1 (dual index) following the manufacturer's protocol.