GSM8717088: MART_IST, rep 2, EM Seq; Homo sapiens; OTHER
Genomic DNA was isolated using the DNeasy Blood & Tissue Kit (Qiagen, 69504) from unsorted untreated cells (UN) or MART-1-specific CD8+ T cells (DC and IST) stained with tetramer for 25 minutes in the dark at a 1:350 dilution prior to isolation by flow cytometric sorting on BD FACSAria™ III Cell Sorter. Genomic DNA extraction and enzymatic conversion were performed using NEBNext Enzymatic Methyl-seq Kit. Converted genomic DNA were submitted to the Hartwell Center at SJCRH for library construction and sequencing on the Illumina NovaSeq platform with 150bp paired-end and target reads of 500 million per sample.