GSM8711823: P-6M-2; Mus musculus; RNA-Seq
Following manufacturer's protocol, the total RNA of sample was extracted using Trizol reagent. After Sample QC, the mRNA was hybridized with Oligo（dT）probe and Captured by Magnetic Beads. The mRNA was then reverse-transcripted to first strand DNA.First strand DNA was used as template to synthesis second strand DNA, obtaining dsDNA. The dTTP tailed adaptor was ligated to both ends of the dsDNA fragments. The ligation product was amplified by PCR, and circularized to get single-stranded circular(ssCir) library. The ssCir library was then amplified through rolling circle amplification (RCA) to obtain DNA nanoball(DNB). The DNB was then loaded to flowcell, and sequenced by DNBSEQ Platform.