GSM9288643: WT Hnrnph2 ASO 3 weeks replicate 3 (3341194_AK063_5); Mus; RNA-Seq
Cortex was dissected, flash frozen and stored at - 80 degrees. Frozen samples were treated with RNAlater-ICE (Thermo Fisher Scientific, AM7030), and total RNA was extracted with Qiagen's RNeasy Universal plus mini kit (73404, Qiagen). A sequencing library was prepared with a TruSeq Stranded Total RNA Kit (Illumina).