GSM8751787: 17hpf_Positive_CNC_3; Danio rerio; RNA-Seq
mch+ CdNC cells and mch- non-neural crest cells from 16 somite-stage Tg(-4.9sox10:GAL4-UAS-cre;UAS:NfsB-mCherry;myl7:nucGFP) embryos were dissected, dissociated and FAC-sorted. GFP+ cells were sorted from 24hpf Tg(-4.9sox10:eGFP) embryos were dissected, dissociated, and FAC-sorted. Tissues were dissociated in Accumax and then sorted BD FACSAria™ Fusion Flow Cytometer. cDNA was prepared from each sample using the SMART-Seq® v4 Ultra® Low Input RNA Kit. Standard Illumina protocols were used to construct each sequencing library and an Illumina HiSeq2500 sequencer at the Millard and Muriel Jacobs Genetics and Genomics Laboratory (California Institute of Technology, Pasadena, CA) for 50 million, single-ended reads.