16SrRNAMetagenome:AFilter1
TheV4regionofthebacterial16SrDNAwasamplifiedusingprimers515F(barcoded)and806R.FollowingAMPuremagneticpurificationbeadsandaQubit-3fluorimeterforpurificationandquantification,equivalentvolumes(8pM)oftheampliconsweresequencedonanIlluminaMiSeqsequencer(usingMiseqreagentkitv3).