GSM9558483: HyPF_scRNA-Seq; Mus musculus; RNA-Seq
Hypothalamus tissues from PF, HS, and QS mice were dissected and enzymatically digested with collagenase II, followed by mechanical dissociation. After dissociation, cell suspensions were collected for downstream single-cell library preparation. Single-cell RNA-seq libraries were prepared using the 10x Genomics Chromium Single Cell 3′ Reagent Kit v3 (10x Genomics) according to the manufacturer's user guide. Briefly, single cells were partitioned into nanoliter-scale droplets and barcoded, and 10x cell barcodes and unique molecular identifiers (UMIs) were incorporated during reverse transcription to associate sequencing reads with individual cells. Following cDNA amplification, sequencing libraries were constructed and sequenced using an Illumina platform. The resulting 10x barcodes were used to assign reads back to individual partitions/cells.