GSM8991229: nuo-6(qm200), L4, biol rep 2; Caenorhabditis elegans; RNA-Seq
Worms were collected, washed three times with M9 buffer to remove bacteria. After removing liquids from the last wash, worms were resuspended in Qiazol, then snap frozen in liquid nitrogen, and stored in a –80°C freezer until RNA extraction.  For RNA extraction, worm samples were disrupted in three freeze thaw cycles with Qiazol between 37°C water bath and snap freezing in liquid nitrogen. Then samples were incubated and vortexed with chloroform at room temperature to extract RNA. RNA was purified through Qiagen RNeasy mini kit following manufacturer's instructions and was treated with RNase-Free DNase on the column to get rid of DNA contamination. All samples had an RNA integrity number of 10. cDNA library was constructed with stranded mRNA (polyA-selected, NEB) library preparation kit following manufacturer's instructions.