RNA-seq of holobiont Myxilla (Burtonanchora) lissostyla: 2020
Four adult individuals of the Antarctic marine sponge Myxilla (Burtonanchora) lissostyla obtained during Dec2020-Jan2021 from Chile Bay, Greenwich Island, South Shetlands, Antarctica were used. RNA was extracted from them using the MirVana miRNA kit with size fractionation, keeping the fraction >200 nt in the sample. A pool of these samples was created through RNA precipitation. The unified sample was purified, quantified and lyophilized for sequencing at Novogene Co., Ltda. For RNA-seq, the sample was enriched in mRNA using oligo-dT, and depleted of rRNA, prior to metatranscriptome library preparation and sequencing with an Illumina NovaSeq 6000, PE150.