GSM9558518: Sulfasalazine3; Escherichia coli O157:H7; RNA-Seq
Bacterial pellet were ressuspended in 1mL of Trizol (invitrogen) reagent and RNA was extracted following the manufacturer's recommendation. After resuspension in RNAse-free water (Ambion), DNAse digestion was performed using the Turbo DNA-free kit (Invitrogen). The resulting solution was then cleaned up using the RNA Clean XP magnetic bead solution (Beckman Coulter). Libraries were prepared using Illumina's Stranded Total RNA Prep Ligation with Ribo-Zero Plus kit and 10bp unique dual indices (UDI)