GSM8594857: MTS20mS1-N; Zea mays; RNA-Seq
About 100 mg finely ground tissues were used for RNA extraction using the Zymo Direct-zol RNA Miniprep Kits (R2052). The RNA quality was checked on a Nanodrop and Agilent TapeStation (5067-5576). The Lexogen QuantSeq 3' mRNA-Seq Library Prep Kit (015.2x96) with UMI (081.96) was used for the mRNA library preparation. The libraries were pooled and sequenced on the Illumina NextSeq 500 platform at NYU-CGSB Genomics Core using the HighOutput 1x75bp mode.