GSM9371176: Decidual_Macro_MEL_1nM_rep4; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions, including on-column DNase digestion when applicable. RNA concentration and purity were assessed by NanoDrop spectrophotometry, and RNA integrity was evaluated using an Agilent Bioanalyzer. Only samples with RNA integrity number (RIN) ≥ 7 were used for library preparation. mRNA libraries were prepared using the MGIEasy RNA Directional Library Prep Kit (MGI/BGI). Briefly, polyadenylated RNA was enriched using oligo(dT) beads, fragmented, and reverse-transcribed to generate first-strand cDNA. Second-strand synthesis incorporated dUTP for strand specificity, followed by end repair, A-tailing, adapter ligation, and PCR amplification. Libraries were quantified and pooled according to manufacturer's recommendations and sequenced on a BGISEQ/DNBSEQ platform with paired-end reads.