Whole Genome Sequencing of Enterobacter hormaechei: Urinary Stone Isolate from 58-Year-Old Female Patient
Genomic DNA was extracted from a pure culture of Enterobacter hormaechei isolated from a urinary stone obtained from a 58-year-old female patient. The DNA was randomly sheared into short fragments, followed by end repair, A-tailing, and ligation of Illumina adapters. The library was size-selected, PCR-amplified, and purified. The final library was quantified using Qubit and qPCR, and fragment distribution was assessed with a Fragment Analyzer. Sequencing was performed on an Illumina NovaSeq 6000 platform using paired-end 150 bp reads.