Whole genome sequencing of Bacillus subtilis
The finished genome is sequenced using second- and third-generation methods, namely Illumina and PacBio. Each sample provides no less than 100 PacBio sequencing data and 100 Illumina sequencing data for the genome, ensuring a more complete and accurate assembly. The finished genome can prevent the loss of small plasmid (<15 kb) information, ensuring the acquisition of a complete genome that includes plasmids.