GSM9669158: S3-R3-s19.9.11; Homo sapiens; RNA-Seq
FD hiPSC-VCMs, WT hiPSC-VCMs, adv-GLA FD hiPSC-VCMs, and adv-GLA WT hiPSC-VCMs were cultured on MatrixPlus-coated 6-well plates (1.2x106 cells/well) for 7 days after adenoviral transduction. hiPSC-CM monolayers were collected in QIAzol, purified with RNeasy columns, and quantitated with a nanodrop spectrophotometer prior to submission to the University of Michigan Advanced Genomics Core. To improve RNA purity and remove potential contaminants that could interfere with downstream enzymatic reactions, samples were further purified using a filter-based spin column repurification protocol as recommended by the sequencing core facility. RNA concentration and purity were assessed using a NanoDrop spectrophotometer, and RNA integrity was evaluated using an Agilent Bioanalyzer. Poly(A)+ RNA libraries were prepared by the University of Michigan DNA Sequencing Core using standard protocols. Library quality and fragment size distribution were assessed using an Agilent TapeStation, and libraries were further quantified by qPCR prior to sequencing.