GSM8991866: Wild-type mouse brain, E15.5, male, sample 3; Mus musculus; RNA-Seq
Three WT and three Aspm GOF embryonic mouse brains were collected from time-pregnant females, dissociated into single cells using Neural Tissue Dissociation Kit (Miltenyi Biotec), and processed the JAX-UConn Single Cell Genomics Center (Farmington, CT). Single cell capture, barcoding, and library preparation were performed using the 10x Genomics Chromium X platform using the Universal 3' Gene Expression GEM-X (v4) chemistry and according to the manufacturer's protocol (#CG000731). Cell counts and viability were assessed on a LUNA FX7 automated cell counter (Logos Biosystems), and >40,000 cells from each input suspension were loaded onto one lane of a 10x Genomics 3'-GEM-X chip. After GEM generation, cDNA and libraries were checked for quality by Tapestation 4200 (Agilent) and Qubit Fluorometer (ThermoFisher), quantified by KAPA qPCR, and sequenced on an Illumina NovaSeq X Plus 100 cycle 10B flow cell, with a 28-10-10-90 asymmetric read configuration, targeting >20,000 barcoded cells with an average sequencing depth of >50,000 read pairs per cell.