Metagenomic data of seawater from coral reefs of Kuantan coastal waters
High quality DNA was fragmented into appropriate sizes and underwent end-repair to facilitate adapter ligation. Next, sequencing adapters containing unique dual indices were ligated to both ends of the DNA fragments through base complementation. A size selection step was performed using magnetic bead purification. The adapter-ligated fragments were subsequently amplified using PCR to increase yield and incorporate index sequences at both ends, thereby completing the construction of the sequencing libraries. The sequencing libraries were validated using a standard QC protocol and subjected to bridge PCR. Finally, paired-end sequencing was performed on the Illumina NovaSeq 6000 platform.