GSM8678030: Korasit dilution 18,000X A; Phanerodontia chrysosporium; RNA-Seq
The fungal mycelium was harvested post 3 days fungal growth on both formulation at two different concentrations using a scalpel, and was immediately snap-frozen in liquid nitrogen. From harvested sample, total RNA from P. chrysosporium was extracted using the RNeasy Plant Mini Kit (Qiagen), following the standard protocol of the provider, with additional Dnase treatment and LiCl precipitation. cDNA libraries were prepared for sequencing using standard Illumina protocols by IGA Technology Services (Udine, Italy)