GSM9521081: naïve lung cells; Mus musculus; RNA-Seq
Lung was excised and cut into small pieces followed by digestion with 1 mg/ml Collagenase I and 0.05 mg/ml DNase I at 37℃ for 45 mins. After passing through a 70 μm cell strainer, red blood cells were lysed. The resulting single cell suspension was added to gel bead-in-emulsions in a DNBelab C4 system (BGI, China) to construct cDNA libraries.