Nanopore sequencing of Pseudomonas aeruginosa cells infected with putative pseudolysogenic bacteriophage
A sequencing library was prepared using the ONT Rapid Barcoding Kit 24 V14 (SQK-RBK114.24). No shearing or size selection was performed. Sequencing was conducted on a MinION Mk1B device using an R10.4.1 Flongle flow cell. High accuracy (HAC) basecalling was performed using Guppy v6.5.7 (E8.2 chemistry, 400 bps model), and barcode demultiplexing was managed within MinKNOW