GSM9371084: A. suci FSLR140051 D3 Rep 2; Alicyclobacillus suci; RNA-Seq
After three days of incubation, 2 mL of the inoculated juice of each strain replicate was placed into a microcentrifuge tube and spun down. The supernatant was discarded and the remaining pellet was washed three times with PBS. RNA extraction was performed using the ZYMO Quick-DNA/DNA Miniprep Plus kit (Zymo Research; Irvine, CA) as directed, with the described DNASE I step included. UDI-barcoded RNAseq libraries were generated with the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs). Each library was quantified with the HS DNA Qubit assay (Thermo Fisher) and the size distribution was determined with a Fragment Analyzer (Agilent) prior to pooling.