Hm.Mock.t11.4
For amplification of the ITS2, 16S-rRNA-V4, 16S-rRNA-V5V7 regions, we used the ITS4_Nextera/5.8SR_Nextera primer pair, V4_515F_Nextera/V4_806R_Nextera primer pair, 799F/1193R primer pair, respectively. To reduce the amplification of plant chloroplast and mitochondrial DNA, Peptide nucleic acid (PNAs) were used for the 16s-rRNA-V4 and 3SpC3 blocking primer for the 16S-rRNA-V5V7 region.