GSM8483420: COLO320DM-3%HD-30min-rep1_MED1-ChIP; Homo sapiens; ChIP-Seq
Cells were crosslinked with 1% FA and 2mM EGS according to the ChIA-PET protocol (PMC8351913). The chromatins were sonicated and immunoprecipitated with anti-MED1 (A300-793A, Bethyl Laboratories), anti-H3K27ac (39133, Active Motif) or  anti-RNAPII antibodies (920102, Biolegend). ChIP DNA and matched input DNA were subjected to end-repair, A-tailing and adaptor ligation (IDT xGen Dual Index UMI Adapters) using KAPA Hyper Prep Kit (KK8505, KAPA Biosystems)