ResC585_Rep3
Total RNA was extracted from homogenised leaf tissue harvested from susceptible, tolerant, and resistant infected and mock-infected plants using the Direct-zol RNA Miniprep Plus extraction kit. The library was prepared using the TruSeq Stranded mRNA LT Sample Prep Kit. The adapter-ligated fragments were amplified through PCR and underwent quality control and quantification processes. The prepared library was loaded onto a flow cell, where the fragments were captured on a lawn of surface-bound oligos complementary to the library adapters. These fragments were then amplified into distinct clonal clusters through bridge amplification.