GSM8483148: COLO320DM_CUT&Tag-ecSEcombo-perturbation-rep1; Homo sapiens; OTHER
CUT&Tag was performed on 50,000 nuclei by using rabbit anti-H3K9me (39765, Active Motif) and CUTANA CUT&Tag Kit (14-1101, Epicypher) as instructed in manufacturer's manual except that digitonin was omitted from buffers as it is not required with use of nuclei (PMID: 32913232). Libraries are prepared based on CUTANA CUT&Tag Kit (14-1101, Epicypher) as instructed in manufacturer's manual.