Identification of tumor-promoting functions of the Homeobox family transcription factor MSX1 in cervical cancer
Identification of tumor-promoting functions of the Homeobox family transcription factor MSX1 in cervical cancer
All steps follow the ChIC/CUT&RUN Assay Kit (Active Motif; Catalog No. 53180) protocol. Birefly: cells (500,000) are pelleted by centrifugation (600 x g, 3 min, RT), supernatant discarded. Nuclei are isolated by resuspending pellets in 100 μL Complete Nuclei Isolation Buffer (Nuclei Isolation Buffer + Protease Inhibitor Cocktail + Spermidine), incubating on ice (10 min), and repeating centrifugation (600 x g, 3 min, RT). All steps follow the ChIC/CUT&RUN Assay Kit (Active Motif; Catalog No. 53180) protocol. Birefly: cells/nuclei are washed twice in Complete Dig-Wash Buffer, resuspended with Concanavalin A beads, and incubated with antibody overnight at 4°C. pAG-MNase is added, followed by washes and chromatin digestion with CaCl2, then DNA is purified using DNA Purification Columns and eluted.  Next-generation sequencing library was prepared using the KAPA Hyper Prep Kit (KR0961–v6.17) according to the manufacturer's instructions and