WGS of Teucrium chamaedrys: leaves from mature plant
Library preparation was done using Roche Kapa HyperPrep DNA Library Kit with Unique Dual Index adapters (Sigma-Aldrich, MO, USA). The completed library was quality assessed and quantified using Qubit dsDNA HS, Agilent 4200 TapeStation HS DNA1000, and the Invitrogen Collibri Illumina Library Quantification qPCR assays. Sample was loaded onto one lane of an Illumina v1.5 S4 flow cell using the Xp Workflow. Sequencing was performed in a 2x150bp paired end format using a NovaSeq 6000 v1.5 300 cycle reagent cartridge (Illumina Inc., CA, USA). Base calling was done by Illumina Real Time Analysis (RTA) v3.4.4 and output of RTA was demultiplexed and converted to FastQ format with Illumina Bcl2fastq v2.20.0.