GSM9290472: HUVEC, 15% strain, replicate 2; Homo sapiens; RNA-Seq
Endothelial cells were isolated from the collagen gel via liberase treatment. RNA was extracted from the cells using the Qiagen RNeasy Mini Kit. RNA quantity and integrity were assessed using Agilent Bioanalyzer prior to library preparation. RNA-seq libraries were prepared using the Takara SMARTer Stranded Total RNA-Seq Kit v3 (Low Input, ZapR-based rRNA depletion; Takara Bio) following the manufacturer's instructions. Library quality was assessed by Bioanalyzer prior to sequencing.