GSM8712323: Ventricular cardiomyocytes, control, rep3; Rattus; RNA-Seq
Total RNA was extracted from isolated ventricular cardiomyocytes using the TRIzol reagent, followed by chloroform phase separation and purification with the RNeasy Mini Kit. RNA integrity and concentration were assessed using a NanoDrop spectrophotometer and Agilent Bioanalyzer to ensure high-quality RNA for downstream processing. Poly(A)+ RNA was subsequently enriched using the NEBNext Poly(A) Magnetic Isolation Module. All steps were performed according to the manufacturer's protocols to ensure reproducibility and sample integrity. Library preparation was performed according to the TruSeq Stranded mRNA library preparation assay