Whole-genome re-sequencing of mutant ML-1
Genomic DNA of ML-1 was extracted from young leaves of two weeks old ML-1 seedling at vegetative stage using Monarch Genomic DNA Purification Kit (NEB, USA). The gDNA was sent to the sequencing service provider and the sequencing was performed using Illumina NovaSeq 6000 platform (Illumina, CA, USA) following the Illumina standard protocol. The 150-bp paired end read library was sequenced to an approximate 40X depth of genome coverage.