GSM9157462: Ribo-seq, 19-28z T cells, RPF 3; Homo sapiens; OTHER
Cells were lysed and 1 µg of total RNA was isolated for RNA-Seq while 9 µg of total RNA was digested with RNase I to isolate ribosome protected RNA footprints (RPFs). Both sample types were taken through rRNA depletion and RNA-seq samples were heat fragmented. Sample types then underwent adapter ligation. Ligated RNA was reverse transcribed, libraries were generated via PCR.