GSM8990184: PFN1_C71G_CTX_Vehicle-4; Mus musculus; RNA-Seq
RNA was isolated from 15 to 20 mg of mouse tissue powder using the Qiagen miRNeasy Mini kit (Qiagen, 217004) and the fully automated QIAcube Connect according to the manufacturer's guidelines. RNA sequencing libraries were generated using the Kapa mRNA HyperPrep Kit (Roche, #KK8581). Libraries were sequenced on a NovaSeq 6000 in paired-end mode, 2x100 bp, generating 106 million mapped pairs in average.