Metagenomic sequencing of sediment sample 33_0m from the southern Gulf of Mexico
Total DNA was extracted and purified from 0.5 g of sediment using the PowerSoil DNA isolation kit (MO BIO-QIAGEN). The DNA was used to amplify the V3-V4 regions of the 16S rRNA gene using the primer pair S-D-Bact-0341-b-S-17 and S-D-Bact-0785-a-A-21. Amplicon libraries were sequenced on the Illumina MiSeq platform using paired-end reads with a 600-cycle configuration.