GSM8595879: MCF7, TIS, replicate 2; Homo sapiens; RNA-Seq
MCF7 and T47D CTR, TIS (at day 12) and REPOP cells were collected by trypsinization, and 15.000 cells/samples were used. Two biological replicates of single-cell suspensions were prepared using Scipio Bioscience's reversible hydrogel technology RevGel-seq™ with the instrument-free 3' scRNA-seq benchtop kit Asteria™, allowing for coupling of solid polymer-barcoded beads and cells in a homogenous phase. Cell capture was followed by cell lysis, mRNA capture on barcoded beads, reverse transcription, PCR amplification and cDNA sequencing. The samples were sequenced to yield approx. 35.000 raw reads per analyzed cell.​ ​ Library was constructed by NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs) with some adaptations according to the instruction of Asteria kit and were then sequenced on the Illumina NovaSeq 6000 sequencer with a medium of 35.000 raw reads per sample.