single-cell RNA-Seq of human induced pluripotent stem cells from a healthy donor and a DMD patient at different time point during myogenic differentiation
single-cell RNA-Seq of human induced pluripotent stem cells from a healthy donor and a DMD patient at different time point during myogenic differentiation
Cells were collected from cultures of iPSCs in 2D culture plates. Dissociation was performed with Trypsin EDTA for 7min at 37Â°C, spinning for 5min at 1000g and fixation / permeabilisation using the Evercode Cell Fixation Kit (Parse Biosciences) Nucleic acid extraction followed the split-pool barcoding protocol commercialized by Parse Biosciences under the name Evercode WT v2 kit Library construction followed the split-pool barcoding protocol commercialized by Parse Biosciences under the name Evercode WT v2 kit. The initial samples included in the experiment were the following: DMD_None_J7; DMD_None_J9; DMD_None_J14; DMD_NC1_J9; DMD_NC1_J14; DMD_MIF_J9; DMD_MIF_J14; DMD_ENO1_J9; DMD_ENO1_J14; DMD_PRTG_J9; DMD_PRTG_J14; H_None_J7; H_None_J9; H_None_J14; H_NC1_J9; H_NC1_J14; H_ID1_J9; H_ID1_J14; H_PDGF_J9; H_PDGF_J14; H_PTN_J9; H_PTN_J14; AP_1; AP_2; AP_3; AP_4; AP_5; AP_6; AP_7; AP_8; AP_9; AP_10. After split-pool barcoding, the samples were pooled, the cells were lyzed and 10 different sublibraries were prepared for sequencing (AP-1 to AP-10). These sublibraries correspond to the 10 samples detailed in this submission. They each contain barcoded nucleic acids corresponding to the 32 cell samples detailed above.