WES of CRC PDX
Genomic DNA was extracted from biological samples using standard protocols (QIagen DNA extraction Kit). DNA was fragmented to an average size of ~150200 bp and sequencing libraries were prepared using Illumina-compatible adapters. Whole exome enrichment was performed using a hybrid capture-based method (Illumina Nextera exome kit), targeting coding regions of the genome. Captured libraries were amplified by PCR and quality assessed prior to sequencing. Single-end sequencing was performed on an Illumina platform (HiSeq 4000), generating reads of approximately 150 bp.