GSM8818849: WT; Mus musculus; ATAC-seq
Cell nuclei were extracted using gradient centrifugation, and DNA was purified using Qiagen PCR (28006) purification kit. ATAC was performed according to ATAC-seq protocol by Biomarker Technologies Biotechnology Ltd. In brief, native nuclei were purified from cell as previously described (Buenrostro et al.,2015). The Nextera DNA Library Preparation Kit (Illumina) was used to perform the transposition according to the manufacturer's manual. 40 000 nuclei were pelleted and resuspended with transposase, for 30 minutes at 37 ℃. The transposed DNA fragments were purified immediately after with a MinElute PCR Purification Kit (Qiagen). After Samples were PCR-amplified using 1X NEBNext High-Fidelity PCR Master Mix (New England Biolabs, MA). Subsequent libraries were purified with the MinElute PCR Purification Kit (Qiagen) and subjected to sequencing on Illumina Novaseq 6000 using PE150.