GSM9777654: Gsk3b (IEC+/+) RNA-Seq, rep1; Mus musculus; RNA-Seq
RNA samples are extracted using TRIzol reagent, typically following the manufacturer's instructions. Sequencing libraries were constructed using the NEBNext® UltraTM RNA Library Prep Kit for Illumina (NEB, USA), adhering to the manufacturer's instructions. Index codes were added to assign sequences to individual samples. The indexed samples were then clustered on a cBot Cluster Generation System using the TruSeq PE Cluster Kit v3-cBot-HS (Illumina), following the provided guidelines. Finally, these RNA-Seq libraries were then clustered and sequenced on an illumina Novaseq 6000 platform (Illumina, San Diego, California, United States) using the PE150 strategy.