WGS of Bacillus safensis: a potential plastic-degrading bacterium
Genomic DNA was extracted from F18 isolate using Monarch Genomic DNA Purification Kit (New England Biolabs). Library preparation was performed using the Twist NGS Library (Illumina; CAT No./ID 104119), where DNA was sheared, adaptor-ligated, and indexed for multiplexing. The library was validated for quality and concentration using 4150 TapeStation System with High Sensitivity D1000 ScreenTape (Agilent Technologies, CA, USA). Sequencing was conducted on the Illumina NovaSeq Plus platform with 2-x 150 bp chemistry to generate ~1.0 Gb/sample. Data was processed using Galaxy version 24.2.rc1.