GSM9557123: WT_PBS HTO_B; Mus musculus; RNA-Seq
After establishing the asthma mouse model, the right lower lung lobe was harvested to prepare single-cell suspensions. A total of 2 x106 single cells from lung tissues were obtained after filtering through a 40 μm cell filter. The single-cell suspension was labeled with BioLegend TotalSeq™-B antibodies (BioLegend, San Diego, CA) after RBC lyses and cell viability tests. The library preparation was performed following the manufacture's guidelines of 10× Genomics in the Single Cell & Transcriptomics Core at Johns Hopkins. After establishing the asthma mouse model, the right lower lung lobe was harvested to prepare single-cell suspensions. A total of 2 x106 single cells from lung tissues were obtained after filtering through a 40 μm cell filter. The single-cell suspension was labeled with BioLegend TotalSeq™-B antibodies (BioLegend, San Diego, CA) after RBC lyses and cell viability tests. The library preparation was performed following the manufacture's guidelines of 10× Genomics in the Single Cell & Transcriptomics Core at Johns Hopkins.