Pacbio sequencing of E coli  chromosome FDN-11 from layer chicken
The bacterial pellet of each sample was resuspended in 180 uL ATL buffer (Qiagen) and was used to extract the high molecular weight DNA using MagAttract HMW DNA Kit (Qiagen). The DNA concentration was evaluated (Table 1) using the Qubit dsDNA HS Assay Kit (Life Technologies) and sample quality was determined using NANODROP 2000 (ThermoFisher Scientific). The size of each gDNA sample was determined using electrophoresis (E-Gel SizeSelect 2% Agarose Gel; Invitrogen), which revealed a fluorescent band > 15Kb respectively for each sample. The samples were then sheared using the Covaris G-tube (Covaris Inc.) and the average size of each sample verified  using the Agilent 2100 Bioanalyzer (Agilent Technologies). Approximately 1 - 1.5 g of gDNA was used as input for library preparation using SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences).