GSM9158991: Gut_2; Mus musculus; ATAC-seq
Epithelial fractions were isolated from mouse small intestine and splenocytes were isolated from spleens. Cells were stained with fluorescent antibodies followed by enrichment of CD45-positive cells with magnetic microbeads. Plasmacytoid dendritic cells (pDC) were sorted from live CD45-positive cells based on lack of staining for CD3 or CD11b and expression of CD11c, Ly6C, and Siglech. Sorted cells were fixed and preparted as ATACseq libraries using a kit from Active Motif (cat #53151).