GSM9202123: Newborn_keratinocytes_BRD4_Rep2; Mus musculus; OTHER
CUT&RUN experiments for H3K27ac, NRF1, KLF3, and BRD4 were performed on freshly isolated newborn keratinocytes using the CUTANA™ CUT&RUN Kit Version 4 (EpiCypher) according to the manufacturer's protocol. Antibodies used for CUT&RUN include: H3K27ac (Active Motif, 39134), and Atlas Antibodies for NRF1 (HPA029329), KLF3 (HPA065054), BRD4 (HPA061646). Libraries were prepared with the NEBNext® Ultra™ II DNA Library Prep Kit (NEB, E7645) and multiplexed using NEBNext® Multiplex Oligos for Illumina (Index Primers Set 1).