GSM9702635: MCF-12A TP53-null LIG1-WT EP1; Homo sapiens; RNA-Seq
RNA was extracted by Qiagen RNeasy Mini Kit. 30uL of 1.5-2ug of RNA was used for construction of sequencing libraries Total RNA was extracted and assessed for quality and quantity prior to library preparation. Strand-specific mRNA sequencing libraries were constructed by Novogene using poly(A) selection with oligo(dT) magnetic beads to enrich for messenger RNA. Purified mRNA was fragmented, followed by first-strand cDNA synthesis and second-strand synthesis under strand-preserving conditions to generate directional libraries. Double-stranded cDNA underwent end repair, 3′ A-tailing, and ligation to Illumina-compatible indexed adapters. Libraries were size-selected, PCR-amplified, and quality-controlled before sequencing. Final libraries were sequenced on an Illumina NovaSeq 6000 platform to generate paired-end 150 bp (PE150) reads.