GSM8482374: N. punctiforme cells, Dusk0, bio rep 1; Nostoc punctiforme PCC 73102; RNA-Seq
Flash-frozen sample pellets were subjected to mechanical lysis, lithium chloride precipitation, and RNeasy Plus Mini Kit and on-column DNase treatment (QIAGEN). Bacterial mRNA enrichment was performed with NEBNext rRNA Depletion Kit (Bacteria) and RNA Sample Purification Beads. Non-directional cDNA libraries were synthesized using Invitrogen SuperScript IV First-Strand Synthesis System before library preparation with the Tecan Life Sciences Ovation Ultralow V2 DNA-Seq Library Preparation Kit. Samples were ran with the Illumina NextSeq 500/550 High Output Kit v2.5 (150 cycles).