GSM8678055: h9-R2-Untreated; Homo sapiens; OTHER
Cells were pelleted and lysed using the provided buffer from Quick-RNA Miniprep Kit (Zymo, R1054) and RNA was isolated per instructions including optional steps. To ensure proper digestion of all plasmid DNA, RNA was additionally treated with 100 U of TURBO DNase I (Thermo Fisher Scientific, AM2238) with appropriate buffering conditions and was subsequently precipitated. Libraries were generated by using error-prone reverse transcription to generate cDNA.  Then libraries underwent gene specific  amplification to adding Illumina adaptors, and a second amplification to add Illumina barcodes.