GSM9126685: Distal small intestine, GFP-intermediate, Replicate 1; Mus musculus; RNA-Seq
Chelation buffer crypt dissociation with TrypLE single cell dissociation. GFP-sorting by FACS in RLTplus + b-mercaptoethanol. Rneasy microkit (qiagen) RNA extraction. A minimum of 30,000 cells for colon and 50,000 cells for small intestine were collected from each GFP fraction. FASTQ files containing the RNA-Seq reads were checked for quality control issues using FASTQC and MultiQC for which no issues were found.