GSM9871710: CD4-GPA-tEPOR, donor 3; Homo sapiens; RNA-Seq
Qiagen RNeasy Plus Micro Kit; REF 74034 RNA-seq libraries were prepared by Novogene. mRNA was purified from total RNA using poly-T oligo-attached magnetic beads, followed by fragmentation and first- and second-strand cDNA synthesis using random hexamer priming. Libraries were prepared by end repair, A-tailing, adapter ligation, size selection, and amplification, then were quantified by Qubit, real-time PCR, and bioanalyzer prior to sequencing on an Illumina platform (paired-end, 150 bp).