GSM8483231: CTRL; Mus musculus; RNA-Seq
Freshly collected ice-cold tissue was transferred into 2 ml of pre-chilled homogenization buffer [15 mM HEPES buffer, 1 mg/mL DNase1 (Roche), and Protector RNase inhibitor at 1:200 (Roche)]. Tissues were carefully homogenized 10-12 times with Dounce homogenizers, filtered with 70 μm cell strainers, and centrifuged at 2,500 rpm for 2 min to pellet cells. scRNA-seq libraries were constructed on the 10x Genomics platform using the Single Cell 3' protocol (v.3.1, 10x Genomics).