GSM9158900: 3xTg, Ramalin 3; Mus musculus; RNA-Seq
Total RNA was extracted from cortex by using RNAiso Plus (Takara). Preparation of mRNA library was conducted by Novogene Co., LTD. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.