Metagenomics of Raja clavata
viral DNA was extracted from spleen tissues of 10 individuals using the High Pure Viral Nucleic Acid Kit (Roche Diagnostic, USA), according to the manufacturers instructions. Viral nucleic acid was enriched for circular DNA molecules using rolling circle amplification (RCA) with the TempliPhi kit (GE Healthcare, USA). RCA products were quantified with Qubit dsDNA HS Assay kit (Thermo Fisher Scientific, USA), pooled equimolarly, and Nextera DNA XT was used for library preparation and sequenced on an Illumina Novaseq 6000 platform.