GSM8678766: TSCL1_Lnc_KO1; Mus musculus; OTHER
The mouse testes nuclei were obtained through the Sucrose density gradient , and MicroSpin S-400 Columns were used for purification of ribosome-protected fragments (RPFs) after Nuclease and RNase Inhibitor treatment. Purify RPFs RNA samples using modified RNA Clean& Concentrator-25 Kit.After enrichment using Oligo (dT) beads, the RNA was fragmented and reverse-transcribed into cDNA using random primers, followed by purification using the QiaQuick PCR extraction kit. After end repair, poly(A) addition and ligation to Illumina sequencing adapters, the ligation products were size-selected using agarose gel electrophoresis, PCR-amplified and sequenced using HiSeq2500 (Illumina) by Gene Denovo Biotechnology.