GSM9050962: me4CL_OTX2-KO-ne3; Homo sapiens; RNA-Seq
Total RNA was isolated using RNA isolater Total RNA Extraction Reagent (Vazyme, R401) according to the manufacturer's instructions. TRIzol was used to extract total RNA, and libraries were constructed using the VAHTS Universal V8 RNA-seq Library Prep Kit (Vazyme, NR605) according to the manufacturer's instructions. 500 ng total RNA was used to construct libraries. Library samples were subjected to the Illumina NovaSeq 6000 sequencing system.