RNA-seq of Shiranuhi
Materials and MethodsTo investigate the differences in transcriptomic dynamics among samples under different treatments, four five-year-old citrus trees (Shiranuhi cv. Hallabong) were planted in three greenhouses (4 meters wide and 12 meters long), each equipped with a built-in temperature control system. The greenhouses were located inside a field house covered with transparent plastic film to simulate orchard conditions during the day (9 AM to 8 PM) and night (8 PM to 9 AM).The temperature treatments applied to each greenhouse were as follows: no treatment control (WT), no treatment but with a slightly higher temperature than the outdoor environment due to its location inside the greenhouse (WT house), daytime +5C heat treatment (DC), and nighttime +5C heat treatment (NC).Growing shoot samples were collected from each treatment and immediately immersed in liquid nitrogen. Total RNA was extracted using the RNeasy Kit (Qiagen, USA), and mRNA sequencing was performed by Macrogen (Seoul, Korea).