WGS of S.cer BY4741 mutant-supresor, sup25
DNA libraries were prepared using the Illumina DNA Prep kit (Illumina, cat. no. 20060059) following the reference guide 1000000025416-10. Input DNA was diluted to 0.5 ng/µl. Indexed libraries were generated using Illumina DNA/RNA UD Indexes (Illumina, cat. no. 20091654). Size selection was performed with Sample Purification Beads (SPB) included in the kit. Library size was validated using a Fragment Analyzer 48-Capillary Array with the HS NGS Fragment Kit (1–6000 bp) (Agilent, cat. no. DNF-474-0500). Libraries were sequenced on a NextSeq 2000 instrument using a P1 XLEAP-SBS Reagent Kit (300 Cycles) (Illumina, cat. no. 20100982), with paired-end dual-indexed reads (2×150 bp).