Transcriptome profiling of peripheral blood from patients with hypertrophic cardiomyopathy (HCM) compared to healthy controls
Transcriptome profiling of peripheral blood from patients with hypertrophic cardiomyopathy (HCM) compared to healthy controls
Peripheral blood samples were obtained from 4 patients with obstructive hypertrophic cardiomyopathy (HCM) and 3 healthy donors. Written informed consent was obtained from all participants, and the study protocol was approved by the institutional ethics committee. Whole blood was collected in EDTA tubes. Total RNA was extracted from whole blood samples using TRIzol reagent. The quality and integrity of the extracted RNA were confirmed using an Agilent Bioanalyzer, ensuring a RIN value greater than 7. Poly-A mRNA was enriched from total RNA for cDNA library construction.