mcherryRNAi_1st rep
The crosses, nosGAL4 with mcherryRNAi (control group) or AccRNAi, (experimental group) were cultured at 18 to limit GAL4 expression during development. Then, male and femalen flies expressing RNAi were collected and cultured at 29 to activate GAL4 expression for 7 days. Ovaries of 30 female Drosophila were collected and total RNA was extracted with Trizol. RNA quantification was conducted to prepare libraries using the SureSelect XT HS2 mRNA Library Preparation kit (Agilent), followed by size selection with AMPure XP beads (Beckman Coulter, USA).