FeFo3_Procypris mera
PCR amplification of the V3-V4 region of the 16S rRNA gene was performed using extracted genomic DNA of intestinal microorganisms as a template, with primers 341F: 5'-CCTACGGGGNGGCWGCAG-3' and 806R: 5'-GGACTACHVGGGGTATCTAAT-5'. Three replicates were conduonally, we used the Total Count Normalization method, which involves dividing each gene count by the total library size and then multiplying by 1,000,000 to get the number of reads per million (RPM) for each gene. The library was sequenced on the HiSeq platform using the PE250 sequencing technology.