GSM8678129: TWGBS, AT4G37110 mutant; Arabidopsis thaliana; Bisulfite-Seq
genomic DNA was extracted from rosette leaves at 9-true-leaf stage using the GeneJET Plant Genomic DNA Purification Kit (Thermo Scientific) and sheared with an E220 Focused-ultrasonicator (Covaris) to achieve an average fragment size of approximately 350 bp. Sequencing libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (New England BioLabs) with methylated adapters (New England BioLabs). The adapter-ligated DNA underwent bisulfite conversion using the EZ-96 DNA Methylation-Gold MagPrep Kit (Zymo Research). Bisulfite-treated samples were amplified using EpiMark Hot Start Taq DNA Polymerase and indexed with NEBNext Multiplex Oligos for Illumina (New England BioLabs). All libraries were sequenced on either an Illumina NextSeq 550 or HiSeq 2500 platform.