Crude 1
rRNA depletion was performed using a custom ordered supplemental probe pool from IDT designed specifically for S. cerevisiae S288C (GCF_000146045.2).  Library preparation was performed using Illuminas Stranded Total RNA Prep Ligation and 10bp unique dual indices (UDI). Sequencing was done on a NovaSeq X Plus, producing paired end 150 bp reads.