GSM9157917: human chondrocyte, DHA-treated 2; Homo sapiens; RNA-Seq
Total RNA was extracted using the Qiagen RNeasy Mini Kit according to the manufacturer's instructions. RNA quality and concentration were assessed using NanoDrop and Agilent 2100/4200 systems. RNA-seq libraries were prepared using polyA-enriched, strand-specific protocols. First-strand cDNA synthesis was performed using random hexamers, followed by dUTP-based second-strand synthesis. Libraries were ligated with sequencing adaptors, PCR-amplified, and purified. Library size distribution was checked via gel electrophoresis, and concentration was quantified by Qubit and qPCR.