TrAEL-seq, Untreated, Replicate 1
For TrAEL-seq, 1  106 1083 asynchronous cells were harvested post-treatment in absence or 1084 presence of HU. Cells were washed in 5 ml L buffer (10 mM Tris HCl (pH 7.5), 100 mM EDTA, 1085 20 mM NaCl), embedded in CleanCut agarose and digested overnight at 50 in 500 l digestion 1086 buffer (10 mM Tris HCl (pH 7.5), 100 mM EDTA, 20 mM NaCl, 1% sodium N-lauroyl sarcosine, 1087 0.1 mg/ml Proteinase K) before washing with TE and PMSF. TrAEL-seq was performed using the updated multiplexing protocol