RNA-seq of Tenmnothorax nylanderi: fat body
We isolated the fat body from each worker by removing the gut and trachea from the first gaster segment, leaving only the fat cells, which were placed in a vial containing 50 l Trizol. Samples were frozen on dry ice and subsequently transferred to -80C.  RNA was extracted using the Qiagen RNeasy extraction kit. We sent the samples to Novogene (Cambridge, UK) for mRNA library preparation using a NEBNext Ultra RNA Library Prep Kit. The libraries were sequenced on an Illumina Novaseq 6000 in paired-end 150 bp mode, yielding a minimum of 9 Gb per sample.