RNA-seq of Pseudomonas Aeruginosa grown under sublethal doses of UVA or in the dark up to early logarithmic phase
RNA-seq of Pseudomonas Aeruginosa grown under sublethal doses of UVA or in the dark up to early logarithmic phase
Mid-exponential cultures (OD650 0.3) were diluted to OD650 0.05 in LB medium and divided into two 30 ml fractions, each of which was placed in a glass beaker (4.5 cm internal diameter). The beakers were placed in a multichamber coupled to a thermocycler bath so that the temperature of the suspensions was maintained at 37Â°C. One of the fractions was irradiated from above at an irradiance of 25 W m2 at the level of the free surface of the suspension, whilst the other fraction was covered with a black plastic sheet (dark control). The cell suspensions were stirred continuously with a magnetic bar.  Cells grown under UVA and their respective controls (see above) were harvested OD650 0.3 and total RNA was extracted by using an RNA extraction kit (Ambion-Life Technology).  The library was prepared with TruSeq Stranded Total RNA with Ribo-Zero beast to remove ribosomar RNA