GSM9204008: CHB donor Hu-P-003720 spatial transcriptomics; Homo sapiens; OTHER
10X Visium FFPE Kit FFPE sections of 5µm thickness were prepared using a Leica RM2255 microtome and placed on Superfrost Plus glass slides (Epredia, Cat#22-042-941), as per guidance outlined in 10X Genomics Visium CytAssist Tissue preparation guide (CG000518). Slides were subjected to H&E staining as outlined in guidance provided by 10X Genomics (CG000518). Following H&E staining, slides were imaged using Aperio AT2 DX scanner (Leica Biosystems, Cat#23AT2100) at 40X magnification. Following imaging, coverslips were removed, and tissues were decrosslinked. Library construction was conducted as described in 10X Genomics, CG000495. Post library construction QC was performed on the 2100 Bioanalyzer Instrument (Agilent) with the High Sensitivity DNA Kit (Agilent Technologies, Cat#5067-4626). Library concentrations were measured using the Qubit dsDNA High Sensitive Assay Kit (Invitrogen, Cat#Q32854). Sequencing was performed on the Illumina NovaSeq6000, following the guidance outlined in the 10X Genomics Visium CytAssist Spatial Gene Expression User Guide (CG000495). Sequencing was performed to meet a targeted sequencing depth of 50,000 read pairs per tissue-covered spot.