GSM8890075: Liver, Combo, replicate 1; Mus musculus; RNA-Seq
At 48-hours of the experiment, livers were perfused with a collagenase solution to dissociate the cells, and live cells were then purified by FACS. Standard 10x Genomics Chromium 3' v2 scRNA-seq reagents were used. Approximately 2000-3000 cells were loaded per channel. Cells from one animal replicate were captured per channel. Standard 10x Genomics Chromium 3' v2 scRNA-seq RT, cDNA amplification, and sequencing library preparation protocols were followed. Multiplexed sequencing libraries were sequenced on Illumina Hi Seq 4000 S1 lanes, averaging about 50,000 reads per cell.