GSM8819822: NP, MG, CR, rep2; Homo sapiens; RNA-Seq
Total RNA extraction and purification was conducted with MirVana PARIS kit (Ambion) by following the manufacturer's instructions. A quantity of 300 ng of total RNA underwent Poly(A) mRNA enrichment with the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #7490). RNA library was built by using the NEBNext Ultra II Directional RNA Library Prep Kit (NEB #E7760/E7765).