GSM8713136: Osm; Mus musculus; RNA-Seq
RNA was harvested using RNeasy Plus Universal Mini Kit (Qiagen). About 0.6 ug of total RNA was used for the construction of sequencing libraries. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dTTP for non strand specific library or dUTP for strand specific library. For the strand specific library, it was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.