RNA-seq of mus musculus
Mouse brains were harvested and preserved in RNA later solution. Total RNA was extracted and sent to Suzhou Hongxun Biotechnologies Co., Ltd. for library preparation using TruSeq RNA Sample Prep Kit v2 (Illumina). Strand-specific, poly(A)-enriched cDNA libraries were sequenced on Illumina NovaSeq 6000 as 150 bp paired-end reads. Raw data were used to correct full-length transcriptome with LoRDEC. Differential expression was analyzed using DESeq2 (v1.20.0). GO and KEGG enrichment of DEGs was performed with clusterProfiler, with gene length bias correction.