GSM9201846: CT27 hTSC-derived EVT-differentiated trophoblast organoids, snRNA-seq; Homo sapiens; RNA-Seq
For extravillous trophoblast (EVT) differentiation, day 11 rotation-cultured hTOr were embedded in Matrigel and cultured in EVT differentiation medium for 14 days according to established protocols. Single-nucleus RNA-seq libraries were prepared using 10x Genomics Chromium Single Cell 3′ v3.1 Dual Index kit. Approximately 10,000 nuclei per sample were used to generate libraries. Libraries were sequenced (150 bp paired-end) on Illumina NovaSeq X Plus.