GSM9130854: Ramos, parental; Homo sapiens; RNA-Seq
Cells were lysed and total RNA was extracted with QIAzol/RNeasy. Total RNA (1 µg) was used for library construction. Poly(A)+ mRNA was isolated using oligo(dT) magnetic beads and fragmented using divalent cations at elevated temperature. First- and second-strand cDNA synthesis was performed using random primers. Double-stranded cDNA underwent end repair, A-tailing, and adaptor ligation. Size selection was performed using magnetic beads, followed by PCR amplification with indexed primers. Library preparation was performed by Genewiz on an Illumina NovaSeq 6000 platform using 2×150 bp paired-end mode.