RNAseq of Atelopus glyphus: Male adult Spleen
We extracted total RNA from the homogenized tissue with EZ2 RNA/miRNA Tissue/Cells Kits (Qiagen) using the EZ2 Connect instrument (Qiagen). We sent RNA extracts to Azenta Life Sciences for poly-A selection, library construction and sequencing. NovaSeq 2x150 libraries (Illumina) were prepared and sequenced on a NovaSeq 6000 instrument (Illumina) to produce ~20 M read pairs per sample.