GSM9048257: 8) Young_NACET_rep4; age 129 days; sex:male; Mus musculus; RNA-Seq
total RNA was purified using EuroGOLD TriFast™ (Euroclone, Milan, Italy). RNA quantity and quality were evaluated by Nanodrop 8000 (Thermo Fisher Scientific, Waltham, MA, USA) and Fragment Analyzer (Advanced Analytical Technologies, Heidelberg, Germany) using the DNF-471 Standard Sensitivity RNA Analysis Kit (15nt) (Agilent Technologies, Santa Clara, CA, USA). RNA samples were  processed for mRNA-seq library preparation using the TruSeq RNA Sample preparation v2 kit according to the manufacturer's instructions (Illumina, San Diego, CA, USA).