ITS Fine mesh BA2 after 8 months
Extracted DNA samples were processed and analysed by Novogene Company Ltd. (UK). Briefly, PCR amplification of the ITS2 region was performed by using ITS3-2024F and ITS4-2409R primers. Quality was determined via 2% agarose gel electrophoresis and products from each sample were pooled, end-repaired, A-tailed, and further ligated with Illumina adapters. The purified amplicons were quantified using the Qubit 4.0 Fluorometer, pooled in equimolar amounts and the final pool was run on the Illumina MiSeq platform to generate 250 bp paired-end raw reads.