GSM8752693: TFiMG64._Sample1_Seq1; Homo sapiens; RNA-Seq
Undifferentiated iPSCs and TFiMGLs at days 2-, 4-, and 6-post induction (both MT and MG media conditions for days 4 and 6) were harvested by first washing with PBS (- calcium, - magnesium) followed by dissociating cultures into single cell suspensions with Accutase (StemCell Technologies, 07920) for 10 minutes at 37°C, cultures were then quenched with DMEM/F12 (Gibco, 11320033) and subsequently centrifuged at 300g for 3 minutes. The cell pellet was rinsed with PBS (- calcium, - magnesium) and centrifuged again at 300g for 3 minutes prior to fixation. Single cell suspensions from each sample were processed using the Parse Biosciences Fixation Kit following manufacturer's instructions (SB1001) and subsequently stored at -80°C until all samples of the time course were harvested. Libraries for all samples were prepared using the Parse Whole Transcriptome Mini V1 kit following manufacturer's instructions (EC-W01010) where an average of 450bp libraries were generated.