GSM8625723: Cnot3;Trp53 dcKO cortex, rep 3; Mus musculus; RNA-Seq
E12.5 embryos were dissected in cold PBS and cortical tissue from single embryos were microdissected and flash frozen on dry ice. Three biological replicates were used per genotype. Tissue was homogenized in RLT buffer (Qiagen) and RNA was extracted using the RNeasy Plus Micro Kit (Qiagen). Libraries were prepared using the TruSeq Stranded mRNA kit (Illumina) according to the manufacturer's instructions