GSM9871664: bone, 20M, GSK, rep1; Mus musculus; RNA-Seq
For single-cell RNA sequencing (scRNA-seq), the single-cell suspension was immediately loaded onto the Chromium Controller (10x Genomics) for automated droplet generation and single-cell barcoding. For bulk RNA-seq, sequencing libraries were constructed using the NEBNext Ultra™ II RNA Library Prep Kit (NEB) following the manufacturer's protocol. For scRNA-seq, sequencing libraries were constructed using the Chromium Single Cell 5' Reagent Kit (10x Genomics) following the manufacturer's protocol. For CUT&Tag profiling, sequencing libraries were generated using the Hyperactive Universal CUT&Tag Assay Kit for Illumina (Vazyme) in accordance with the manufacturer's instructions.