RNAseq of USA300: caMHB
Overnight cultures of S. aureus USA300 were diluted to 0.1 OD600 in caMHB or SWF, grown for 6h, and diluted to 0.8 OD600. The cultures were washed by centrifuging at 10,000 rpm for 10 min. Cell pellets were then resuspended in PBS, centrifuged at 10,000 rpm for 10 min, snap frozen, and sent for RNA extraction and sequencing by Genewiz
SUB16290812
SAcaMHB