22Rv1_AS
The 22Rv1 (RRID: CVCL_1045) cell was obtained from the ATCC and grown in RPMI1640 medium supplemented with 10% fetal bovine serum (FBS). After harvesting the cell during log phase, we used Puregene Cell Kit (QIANGEN, 158043) to extract high-quality genomic DNA with RNase A treatment included. The genomic DNA was sheared into 8 kb fragments with g-TUBE (Covaris, 520079). The sheared DNA was used for PCR-free nanopore sequencing library preparation using Ligation Sequencing Kit (Oxford Nanopore Technology, SQK-LSK110). After library preparation, 100 fmol library was loaded to R9.4.1 flow cell. With the target BED file and the matched reference FASTA plugged in, we started the data acquisition in MinKNOW and kept running until the flow cell efficiency fully depleted.
SUB14711475
22Rv1_AS_bam