Genomic DNA sequencing for chloroplast genome assembly of Haiyan Datoucai, an import economic cultivar of Brassica juncea var. napiformis (Pailleux & Bois) Kiatm
Fresh leaves of Haiyan Datoucai were collected from Jiaxing Academy of Agricultural Sciences, Jiaxing City, Zhejiang Province, China (coordinates: 304955N1204259E). The collected specimen, labeled HYDTCJ001, is preserved at the Institute of Eco-Environmental Sciences, Jiaxing Academy of Agricultural Sciences (https://www.jxnky.com) under the stewardship of Zhangliang Yao (zlyao513@126.com) and Yeqing Wang (909247083@qq.com). Total genomic DNA was extracted from fresh leaves using the CTAB method (Doyle and Doyle 1987). The extracted DNA was used to construct a sequencing library, which was sequenced on an Illumina NovaSeq 6000 platform (Illumina Inc., San Diego, CA, USA) with paired-end reads of 150 bp.
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