ITS rRNA gene sequencing of roots DNA from natural stands with Populus tremuloides: sample FORE.004S.Roots
Libraries for sequencing of the fungal ITS2 region were prepared according to Illuminas 16S Metagenomic Sequencing Library Preparation guide, with the exception of using Qiagen HotStar Taq DNA polymerase for the first PCR (amplicon PCR). The template specific primers were (without the overhang adapter sequence): ITS9F (5- GAACGCAGCRAAIIGYGA-3) and ITS4R (5- TCCTCCGCTTATTGATATGC-3).
SUB15136754
055-FORE-004S-Root-ITS