18S rRNA gene sequences of a Populus deltoides wislizeni leaf pack submerged in the middle Rio Grande
DNA was extracted using a modified CTAB method with phenol:chloroform:isoamyl alcohol purification; PCR was performed in triplicate to amplify the V9 region of the 18S rRNA gene using Illumina 1391f and EukBr primers; SequalPrep was used to clean up and normalize PCR products; a Nextera XT DNA Library Preparation Kit was used to index and prepare sequencing libraries; libraries were purified following a AMPure XP bead cleanup protocol; the dual-indexed, paired-end amplicons were sequenced on an Illumina MiSeq at the University of New Mexico
SUB15448748
FPBxR66xLPx08x1391fEukBr