Illumina sequencing of wMel Wolbachia isolated from Drosophila melanogaster JW18 cells
Wolbachia was isolated from host cells by pelleting the cells (1,000  g for 5 min), vortexing the pellet with sterile glass beads (2-sec pulses until fully dispersed), and filtering the resulting lysate through a 5.0-m Ultrafree-MC-SV Durapore PVDF filter (20,000  g for 10 min at 4C). High-molecular-weight DNA was extracted from isolated Wolbachia using the Zymo Quick-DNA HMW MagBead Kit, following the manufacturers instructions for cells and solids. Illumina paired-end libraries were prepared using the Nextera Small Genome DNA Library protocol (LIB-146b) and sequenced on an Illumina NextSeq 2000 with a P2 flow cell (100 cycles, v3 chemistry)
SUB15233251
IL_JW18_wMel