LaminB1 GO-CaRT on RG purified from GW20 cerebral cortex (rep2)
Radial glia (RG),  Intermediate progenitor cells (IPC) and Excitatory neurons (EN) were purified from human cortical brain tissue at gestational weeks (GW) 20 using flourescence activated nuclei sorting (FANS).  Sorted nuclei were incubated with Fab fragments to block antibodies used in FANS and ~25,000 nuclei were  subjected to GO-CaRT using antibodies to nuclear lamina intermediate filament protein, LaminB1. Sequencing libraries were constructed using KAPA Hyperprep kit following manufacturer's instructions.  Libraries were sequenced using 150-bp paired-end sequencing on Illumina NovaSeq X Plus.
NIMH-phs000989
GW20_141_RG_LMNB