Enzymatic Methyl-seq of prostate cancer: adult male
EM-Seq libraries were prepared with 10-200ng of DNA using the NEBNext Enzymatic Methyl-seq Kit and NEBNext Multiplex Oligos for Enzymatic Methyl-seq Unique Dual Index Primer Pairs (New England BioLabs, Ipswich, MA, USA) according to the manufacturers protocol.  Sample integrity and absence of degradation were verified via spike-in controls of Lambda DNA (Thermo Fisher Scientific, Waltham, MA, USA) and pUC19 plasmid (New England Biolabs, Ipswich, MA, USA). Library quantification was performed using the Qubit 2.0 fluorometer. Library quality and size distribution were assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), followed by paired-end sequencing (2  150 bp) on an Illumina NovaSeq 6000 platform (Illumina, San Diego, CA, USA).
SUB15626405
N1764