16SrRNA sequecing
Following amplification and purification of the 16S rRNA gene with Agencourt AMPure XP beads (Beckman Coulter, USA), the PCR amplified was quantified using the Qubit dsDNA assay kit and Qubit (v.3) fluorometer (Thermo Fisher Scientific, USA). Following quantification, the 16S rRNA amplicons were combined in equal quantities for library construction with the Prep Kit 2.0 (Biosciences, USA), per the manufacturer's guidelines. The constructed 16S rRNA gene libraries from each pooled and barcoded sample were sequenced on a PacBio Sequel II 8M platform (Bioscience, USA), following company protocol.
SUB14675737
FYZX.5