PCR-cDNA seq of Mustela putorius furo: in vivo IAV-infected 24H 3
One adult outbred ferret (600-1500 gram) was inoculated intransally with seasonal influenza-A (IAV) A/Perth/265/2009 (Perth265, H1N1pdm09) via 500 uL of PBS containing 107 PFU of Perth09. At 24 hpi, the ferret's nasal tissue was collected. The RNA was extracted using the RNeasy Mini kit, treated with DNase (Turbo Dna-Free Kit). The sequencing was carried out using the PCR-cDNA Barcoding Kit (SQK-PCB111.24, Oxford Nanopore Technologies), via the GridION for 72 hours.
SUB15371013
Vivo_IAV_24H_3