RNA-seq of badder tumor
Take a certain amount of total RNA samples, and use oligo dT beads to enrich mRNAwith poly A tail;2. mRNA molecules were fragmented into small pieces;3. The fragmented mRNA was synthesized into first strand cDNA using random primers;4. The second strand cDNA was synthesized with dUTP instead of dTTP;5.The synthesized cDNA was subjected to end-repair and 3' adenylated. Adaptors wereligated to the ends of these 3' adenylated cDNA fragments;6.Digest the U-labeled second-strand template with Uracil-DNA-Glycosylase (UDG) andperform PCR amplification;7. Library quality control;8. Library circularization;9. The library was amplified to make DNA nanoball (DNB);10. Sequencing on DNBSEQ (DNBSEQ Technology) platform.
SUB15081983
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