IgG control for 137_GW16_GO EN
adial glia (RG),  Intermediate progenitor cells (IPC) and Excitatory neurons (EN) were purified from human cortical brain tissue at gestational weeks (GW) 16 using flourescence activated nuclei sorting (FANS).  Sorted nuclei were incubated with Fab fragments to block antibodies used in FANS and ~25,000 nuclei were  subjected to CUT&RUN using  antibodies to using IgG antibodies as controls.. Sequencing libraries were constructed using KAPA Hyperprep kit following manufacturer's instructions.  Libraries were sequenced using 150-bp paired-end sequencing on Illumina NovaSeq 6000.
NIMH-phs000989
GW16_EN_IgG