polyA_run1
The sRNA sample was poly(A)-tailed using poly(A) polymerase and 5'-PPP structures were removedusing tobacco acid pyrophosphatase. Afterwards, a RNA adapter was ligated to the 5'-monophosphateof the RNA. First-strand cDNA synthesis was performed using an oligo(dT)-adapter primer and M-MLVreverse transcriptase. The resulting cDNA was PCR-amplified to about 20-30 ng/ l using a high fidelityDNA polymerase. PCR cycles performed and the barcode sequence, which is part of the 3'-TruSeqsequencing adapter, is included in Table 2. The cDNA was purified using the Agencourt AMPure XP kit(Beckman Coulter Genomics)
SUB14703285
polyA_run1