Gut 16S sRNA V3-V4 of Vespa velutina nigrithorax: larvae
Genomic DNA from gut was individually extracted and purified using the Qiagen DNeasy Blood & Tissue kit (Qiagen) following the manufacturers instructions. 16S rRNA V3-V4 region  was amplified using the primer pair Pro341F (5-CCTACGGGNBGCASCAG-3') and Pro805R (5- GACTACNVGGGTATCTAATCC-3'). Library preparation was performed using the Nextera XT kit, and sequencing was carried out on the Illumina MiSeq platform, generating 275 bp paired-end reads.
SUB15390910
DIG_034_16S_rRNA_V3_V4_Pro341F