GBS of Cichorium intybus: sample 'Variegated of Chioggia isolate 8'
double digestion of gDNA with PstI and MspI restriction enzymes, ligation to sequencing adapters with barcodes, pooling, enrichment by PCR, size selection with beads to 100-600bp fragments length, sequencing on ionTorrent platform Ion GeneStudio S5 Plus (protocol adapted from Abed et al., 2019)
SUB14887729
ChV4_8