Dap-Seq of OryzaSativa Lsppjaponica:sample5
DAP-seq libraries were prepared by cloning rice Dof23 CDS into pET vector, expressing HIS-tagged protein in vitro, and incubating with sheared rice genomic DNA (200-500bp). Protein-DNA complexes were enriched using Ni-NTA beads, followed by library construction (NEB Next Ultra II Kit) and PE150 sequencing on Illumina NovaSeq 6000 (2 technical replicates + input control). Data were analyzed using BWA-MEM, MACS2, and MEME.
SUB15487120
Rice_dof23_dap_seq_L1LGH1700022_1