Single-cell seq of prostate tissue of AS pt 177 (set 2 of 4)
Fresh tissue biopsies were acquired from patients newly diagnosed with prostate cancer. Tissue cells were processed immediately after sample acquisition. Cells were extracted from tissue enzymatically and mechanically. The cellular samples were loaded in the lane according to the 10X Genomics Chromium Next GEM Single Cell 5'v2 (Dual Index) User Guide with a single capture lane per sample. Reverse transcription PCR was run overnight. All subsequent steps of library preparation and quality control were performed as described in the 10X user guide.
NCI-phs001813
SCAF3306_S9_L002