RNASeq on Cneoformans treated with CDK7 inhibitors
Cryptococcus neoformans H99 cells were grown overnight in YPD at 30C, diluted to OD600 = 4, and allowed to recover for 2h in fresh YPD. Cells were then treated with 50g/mL BS-181 for 1h in biological triplicates. Samples were harvested by centrifugation, snap-frozen in liquid nitrogen, and total RNA was extracted using TRIzol, followed by purification with Qiagen RNeasy Mini Kit. RNA integrity was assessed using Agilent TapeStation (RIN >8). Stranded, poly(A)-enriched RNA-seq libraries were prepared using the Illumina mRNA Prep Kit. Libraries were indexed, quality-checked, pooled, and sequenced on an Illumina NovaSeq X 300 platform (150bp paired-end reads) at AGRF.
SUB15735360
WT_BS181_1h_1_22JKVGLT3_ACAGTGTATG-GAACATACGG_L005