16S seq Cheese: Fcheese4
DNA was extracted using the Qiagen DNeasy PowerFood Microbial kit and amplified using 341F (ACTCCTACGGGAGGCAGCAG) and 806R (GGACTACHVGGGTWTCTAAT) primers. The library was prepared using 2  Phanta Max Master Mix polymerase and final library was denatured, circularized, an amplified with phi29 polymerase to yield DNA nanoballs (DNBs), and the sequenced using the DNBSEQ-G400 platform
SUB15359113
F-Cheese4