Raw reads: Mutation Accumulation line 39 (generation 32) HiFi reads
HMW DNA was extracted at the Max Planck Institute for Biology Tuebingen using a modified protocol, including beta-mercaptoethanol during lysis and a phenol purification step (Rabanal et al. 2022). DNA was further purified using two rounds of bead cleanup (SeraMag SpeedBeads and AMPure PB beads). Libraries were prepared using the SMRTbell prep kit 3.0. HiFi sequencing was performed on the PacBio Revio device at Max Planck-Genome-Centre Cologne (MP-GC).
max planck institute for biology tuebingen
webin-reads-Mutation Accumulation line 39 (generation 32) HiFi reads