pre-treatment
DNA libraries were prepared following the Illumina DNA Prep Reference Guide (1000000025416-10) using the Illumina DNA Prep kit (20060059). Input DNA was diluted to 0.5 ng/l prior to tagmentation. Unique dual indexes were added using the Illumina DNA/RNA UD Indexes (20091654). Size selection was performed with Sample Purification Beads (SPB) according to the manufacturers instructions. Library fragment size was validated using a Fragment Analyzer 48-Capillary Array with the HS NGS Fragment Kit (16000 bp) (Agilent DNF-474-0500).
SUB15970081
A2