hPADI2 PhotoRaPID
DNA libraries, including 4-8 degenerate NNK (5-TAATACGACTCACTATAGGGTTAACTTTAAGAAGGAGATATACATATG(NNK)nTGCGGCAGCGGCAGCGGCAGCTAGGACGGGGGGCGGAAA) were subjected to 6 rounds of RaPID selection using hPADI2 as protein target and different buffer conditions. Double indexed libraries (Nextera XT indices) were prepared with the DNA recovered after each round and sequenced on Illumina NovaSeq X Plus with a 10B Flowcell size and 100 nt paired-end reads (200 cycles total)
SUB16260997
hPADI2_C2pnt2_4_8_R5_Z