fungal community of pine needle tissue
The outside of needles were sterilized, ground with liquid nitrogen and bead beating, and DNA was extracted with the SYNERGY 2.0 DNA extraction kit with  the addition of a chloroform:isoamyl alcohol (24:1) step to remove organic compounds. Fungal community profiling was carried out by Novogene using the priemrs and ITS4-2409R for the ITS2 region.
SUB16036257
S3_34_24S_sample_ITS