whole exome sequencing of blood sample from patients treated with glucocorticoid medications
DNA was sheared to 385bp,  size selected and prepared using KAPA Hyper Prep Biosystems with Library Amplification Primer Mix with palindromic forked adapters using unique 8-base index sequences embedded within the adapter. Cleanup was done using AMPure XP beads. Hybridization and capture were performed using IDT's XGen hybridization and wash kit. For capture custom exome bait using TWIST Biosciences was used. Libraries were sequenced on the NovaSeq XP with 2X151 bp reads.
NEI-phs004037
000010562448F10_Illumina_P5-Xayol_P7-Pepac