DNA-Seq of bioleaching batch experiments
DNA was extracted from the tailings after bioleaching experiments at day 0, 7, 15, and 30 to monitor the microbial shift. Popidium monoazide was used to differentiate from active and non-active cells. The V3 and V4 regions were amplified using 16S (F: 5 TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG3 and R: 5 GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG3) primer pairs
SUB15808374
NSTCS D0 PMA+