metagenome of sea water
The quality of DNA was examined by Synergy HTX Multi-Mode Reader (Agilent) and the amplified products were checked with 1.8% agarose gel. 16S rRNA gene amplicon analysis was conducted by using illumina novaseq6000 (novaseq6000, illumina) with primer 338F (5'- ACTCCTACGGGAGGCAGCA-3') and 806R (5'- GGACTACHVGGGTWTCTAAT-3) in Biomarker Technologies CoLTD.
SUB14655162
14