16S DNA sequecning of the metagenome of the phyllosphere and thatch of turfgrass treated with different levels of nitrogen fertilizer
The sequencing library for prokaryotic communities was prepared by amplifying the V4 region of the 16S rRNA gene using the 515f/806r primers. PCR was performed with the KAPA Biosystems PCR kit, and products were cleaned using the ZR-96 Zymoclean Gel DNA Recovery kit. The amplicons were normalized with the Mag-Bind EquiPure Library Normalization kit, pooled in equal volumes, and quantified using a Qubit 3.0 Fluorometer. The final library was diluted to 4 nM before sequencing on the Illumina MiSeq platform using a 2  250 bp paired-end approach.
SUB15073356
Nitrogen_Microbione_Library2017_38