Cut&Tag of Drosophila
The CUT&Tag experiment was conducted using the NovoNGS CUT&Tag 4.0 High-Sensitivity Kit (Vazyme, Nanjing, China, catalog number N259-YH01), following the manufacturer's protocol and employing the H2Av antibodies (UNC93-5.2.1, DSHB). Finally, Qubit was utilized to measure the concentration. Concentrations are determined using Qubit assays. The library fragments are then assessed with the Agilent Technologies 2100 Bioanalyzer and sequencing with Illumina.
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