SUM159 cell line gRNA library amplified for CRISPR screen analysis
Genomic DNA (GeCKO v2 Library) was isolated from the harvested cells/tumors. Integrated gRNA sequences were amplified following the protocol described in Joung et al., Nature Protocols 12, 828863 (2017) (DOI: 10.1038/nprot.2017.016). The necessary Illumina P5 and P7 flow cell adapters and unique sample-specific indices were added. Final libraries were purified and sequenced on an Illumina HiSeq 2500
SUB15775962
Cell_Lib2