HybSeq of Aureonarius limonius
Dual-indexed genomic libraries were pooled and hybridised with our custom myBaits enrichment panel, following v3.0 manufacturers protocols, with the exception of pooling four or nine libraries per hybridisation reaction and each reaction having a total of  of the recommended volume. Hybridisations were performed at 65C for 20 hours in Vapo Protect Mastercycler 6325 thermocycler (Eppendorf, Arlington, UK). Captured targets were amplified with a KAPA HiFi 2x HotStart ReadyMix PCR kit (Roche, Basel, Switzerland) for 10 cycles, and the PCR products were cleaned using Agencourt AMPure XP magnetic beads. Final products were quantified and qualified as above. Thirteen enriched libraries were further pooled for sequencing on an Illumina MiSeq platform (Illumina, San Diego, CA, USA) using v2 Nano chemistry (2  250 bp paired-end reads) at the Jodrell Laboratory, Royal Botanic Gardens, Kew
SUB15394044
Aur_lim