Metabarcoding of kettle hole soil DNA extracts
DNA was extracted from soil samples with DNeasy PowerLyzer Soil kit (Qiagen). The two-step 16S rRNA gene PCR protocol targeting both archaea and bacteria followed a previously established protocol (Frindte et al., 2023), with the distinction of using primers 515fB (Parada et al., 2016) and 806rB (Apprill et al., 2015) in the first and 515f (barcoded) and 806r (Caporaso et al., 2012) in the second round.
SUB15809830
Sm05_16S