RNA-Seq of mus musculus: normal chondrocyte
Cultured primary mouse chondrocytes were incubated at 37C with 5% CO2 for 4 days and harvested on day 5. Total RNA was extracted from reagent-treated chondrocytes using TRIzol (MRC; TR-118) and chloroform (Sigma; 319988). The isolated total RNA was reversed transcribed, and cDNA was synthesized using the ImProm-II Reverse Transcriptase kit (Promega, A3803).
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