RNA-Seq of WT Anopheles gambiae: adult ovaries
Ovaries from 24 days post-emergence, unmated homozygous nanosd and wild-type females were dissected. Ovary samples were homogenised with a sterile pestle, and RNA extractions were performed using the Direct-zol RNA Microprep kit (Zymo Research), including the DNase treatment step. A total of 30 ovary pairs per line were collected for three biological replicates. The mRNA samples were purified using poly-T oligo-attached magnetic beads, and cDNA libraries were generated by using dUTP. The cDNA libraires were sequenced on a NovaSeq X Plus Illumina platform, generating 150 bp paired-end reads.
SUB15593058
ERAS240054156-1r