scRNA-Seq for human melanoma
The cells from each patient were labeled with sample tags from the Human Immune Single-Cell Multiplexing Kit (BD Biosciences), following the manufacturer's protocol. The cell capture beads were retrieved for reverse transcription as per the manufacturers protocol (BD Biosciences, Single-Cell Capture and cDNA Synthesis). Single-cell capture and cDNA library preparation were performed using the BD Rhapsody Express Single-Cell Analysis System (BD Biosciences), following the manufacturer's protocol. The libraries were loaded on a S1 flow cell (2  100 cycle) and paired-end sequenced at >200,000 reads per cell depth using a Novaseq 6000 Sequencer (Illumina) at the Kinghorn Centre for Cellular Genomics, Garvan Institute, Sydney, Australia. PhiX (20%) was added to the sequencing run to compensate for the low complexity library.
SUB14853587
R5