RNA-Seq of Caproiciproducens galactitolivorans : cultivation with glucose under argon
Libraries were prepared from Bacterial RNA using the CORALL RNA-Seq V2 Library Prep Kit(LEXOGEN, Inc., Austria). rRNA was removed using RIBO COP rRNA depletion kit (LEXOGEN,Inc., Austria). The rRNA depleted RNAs were used for the cDNA synthesis and shearing, followingmanufactures instruction. Indexing was performed using the Illumina indexes 1-12. The enrichmentstep was carried out using of PCR. Subsequently, libraries were checked using the Agilent 2100bioanalyzer (DNA High Sensitivity Kit) to evaluate the mean fragment size. Quantification wasperformed using the library quantification kit using a Step One Real-Time PCR System (LifeTechnologies, Inc., USA). High-throughput sequencing was performed as paired-end 100 sequencingusing NovaSeq 6000 (Illumina, Inc., USA)
SUB14654681
BS1_GLU_Argon