PacBio HiFi sequencig of Prasinohaema aff. flavipes: adult female liver
High molecular weight (HMW) genomic DNA (gDNA) was extracted from Prasinohaema aff. flavipes using a Qiagen genomic tip DNA extraction kit. HMW DNA was sheared to 18kbp using a Diagenode Megaruptor, with the BluePippin system (Sage Science, Beverly, MA, USA) collecting fractions containing >15-Kbp fragments used for library preparation. Genomic libraries were prepared following the SMRTbell Express Template Prep. Kit 2.0 protocol (PacBio, PacBio, Cat. #100-938-900). The library was then sequenced on a single flow cell on the PacBio Revio instrument.
SUB14881734
BYU:Herp:5520_lib1