WGS of Gonocytisus angulatus
The NucleoSpin Plant II Mini kit (Macherey-Nagel, Dren, Germany) was used for whole genomic DNA isolation. If the NucleoSpin kit was unsuccessful, genomic DNA was extracted using a modified cetyl trimethylammonium bromide (CTAB) method (Doyle & Doyle, 1987) with the addition of 2% Polyvinylpyrrolidone-40 (PVP) and 2% betamercaptoethanol (BME) added to the extraction buffer. The resulting pellet was resuspended in DNAse-free water and stored at -20 C for genomic sequencing. DNA concentration was checked using a NanoDrop spectrophotometer and agarose gel electrophoresis before choosing DNAs for Illumina sequencing at the Beijing Genomics Institute (BGI).
SUB14612056
GoAn_55