transcriptomic sequencing
In this research, we aimed to induce an immune response in Hyalomma asiaticum by exposing it to both Staphylococcus aureus and Escherichia coli stress. Through this approach, we monitored the gene - level changes during the tick's immune reaction. Our primary goal was to identify and structurally elucidate the genes encoding antimicrobial effector molecules of H. asiaticum defensins that are specifically up - regulated in response to particular pathogen invasions. To achieve this, a data - mining strategy was employed. By analyzing the gene expression data obtained from the bacterial - stressed ticks, candidate molecules that could be further investigated in in vitro antibacterial experiments were sought to be pinpointed. It is anticipated that these findings will offer fresh perspectives on the defense mechanisms employed by ticks against bacterial threats.
SUB15481728
PBS3