Kpneumoniae_BAC-resistant_sample_1
Library prep performed via DNA fragmentation, end repair and A-tailing, adapter ligation, PCR amplification and sample purification. Library quantification performed using a Quibit, qPCR to validate illumina anchor seqs. QA performed by Agilent 2100 Bioanalyser. sequenced using an Illumina NovaSeq 6000. Image data transformed to raw reads by CASAVA base calling, QC conducted to discard read pairs if adapter contamination, uncertain nucleotides >10%, or >50% low quality nucleotides. Clean paired-end reads mapped to ref genome (NCBI entry: 32868_D02, GenBank assembly GCA_900451585.1) by Burrows-Wheeler Aligner.
SUB15359087
KpBAC1