RNA-Seq of Homo sapiens: U251
The concentration and purity of the extracted RNA were measured using a Nanodrop 2000. mRNA was isolated from total RNA through A-T base pairing between the polyA tail and oligo(dT) magnetic beads. The mRNA was then randomly fragmented into approximately 300 bp pieces. First-strand cDNA was synthesized through reverse transcription. An End Repair Mix was added to create blunt ends. The product was purified and size-selected after adapter ligation, followed by PCR amplification to generate the final library. The library was subsequently subjected to sequencing on an appropriate platform.
SUB15629286
CTSZ-KD-bio2