A_April2018_coral18
DNA was extracted from both coral tissue, sediments and the water samples using the Qiagen DNeasy Blood and Tissue kit using the manufacturers protocol. ITSS rRNA genes from cDNA were amplified using the primers CS1-5.8S-Fun-2-for (5-CAAGCAGAAGACGG CATACGAGAT-NNNNNNNNNNNN-AGTCAGTCAG-GG-AACTT TYRRCAAYGGATCWCT-3) and CS2-ITS4-FUN-2-rev (5=-AATGATACGGCG ACCACCGAGATCTACAC-TATGGTAATT-AA-AGCCTCCGCTTA TTGATATGCTTAART-3). PCRs were carried out in 25l reaction mixtures with 5l of template DNA using illustra PuReTaq ready-to-go PCR beads (GE Healthcare Life Sciences, Pittsburgh, PA, USA). The extracted DNA was sent to the sequencing facility for quality control, PCR, library preparation, and pair-end sequencing with Illumina MiSeq platform v3 (2  300 bp).
SUB14855299
40