Amanica praecellens isolate IS5
The DNA was extracted with a column-based kit (Tissue Genomic DNA Mini Kit, Geneaid). Initially sequenced with Levantina menkhorsti and Isaurica schuetti on a single Illumina MiSeq run (paired-end, 75 bp), but that was sufficient only for the Levantina sample, so the libraries from A. praecellens and I. schuetti were run again, sharing the second run in the ratio 1:2. The raw reads were trimmed and quality filtered with Cutadapt 4.6 using TrimGalore and deduplicated with fastp; reads from the two sequencing runs were then combined.
SUB14647086
amanica