Oxford Nanopore sequencing of Clemmys guttata: adult female blood
An ultra-long (UL) nanopore sequencing library was prepared using an Ultra-Long DNA Sequencing Kit (V14; Oxford Nanopore Technologies [ONT], Oxford, UK) following the manufacturers protocol with extended incubation times for DNA damage repair, end repair, ligation, and bead elution. 20 l of whole blood was used as input into a Monarch Extraction Kit for Tissue (New England Biolabs, Ipswich, MA). 30 fmol of the final UL library was sequenced using an R10.4.1 flow cell on a PromethION P24 device set up using MinKNOW - PromethION 23.07.12. Data were basecalled live during the run with dorado-for-promethion 7.1.4. The flow cell was washed with a flow cell wash kit (ONT) and a fresh library was loaded at 24 hours.
SUB15684660
CLGU-ONT2