cynaobacteria-targeted amplicon of Epilithic cyanobacterial biofilm community scraped from benthic rocks in the Quinnipiac River at Sindall Road in June, rep 2
All samples were filtered through 0.2 m polycarbonate membranes. Collected filtrate was directly used for genomic DNA extraction using the DNeasy Soil Power Kit (QIAGEN, Germantown, MD, USA), according to the manufacturers instructions. Extracted DNA samples were sent to Novogene Corporation Inc. (Sacramento, CA, USA) for PCR amplification and sequencing. Cyanobacterial 16S rRNA genes were amplified using the primer set CYA359F and CYA781R (Nubel et al. 1997). Sequencing libraries were prepared using the Illumina TruSeq DNA PCR-Free Sample Preparation Kit (Illumina, Inc. San Diego, CA, USA) under Novogenes standard protocols.
SUB16327538
NS050