Shotgun metagenomic sequencing of DHS
A shotgun metagenomic sequencing library was prepared from the extracted DNA according to the manufacturers protocol and sequenced on a DNBSEQ-G400 platform (MGI Tech) using paired-end sequencing chemistry to generate 150-bp paired-end reads.Raw reads were quality-trimmed using fastp v0.20.1 and assembled de novo using SPAdes v3.15.5. The resulting contigs were binned into MAGs using MaxBin2 and refined using Binning_refiner. MAG quality was assessed using CheckM v1.0.12, and relative abundance was estimated using CoverM. Genome annotation and phylogenetic placement were performed using DFAST and PhyloPhlAn3, respectively.
SUB16181231
DHS_D224_Top_Outer