RNA-Seq of Saccharum spp.: root
Total RNA was extracted from leaf tissues using the TRIzol reagent, and RNA integrity was assessed using an Agilent 2100 Bioanalyzer. mRNA was enriched by poly(A) selection, fragmented, and reverse-transcribed into cDNA. Libraries were constructed using the NEBNext Ultra II RNA Library Prep Kit and sequenced on an Illumina NovaSeq 6000 platform to generate 150 bp paired-end reads.
SUB15471529
GL05-136-R-T3