tRF-Seq of Eriocheir sinensis: hemocytes treated with Aeromonas hydrophila rep 1
Hemocytes were harvested from Chinese mitten crab (Eriocheir sinensis) treated with Aeromonas hydrophila or PBS. Total RNA was isolated and size-fractionated using polyacrylamide gel electrophoresis (PAGE) to select small RNA fragments ranging from 15 to 45 nucleotides. To remove RNA modifications for tRF sequencing (PANDORA-Seq), RNA pretreatments were conducted. Small RNA libraries were subsequently constructed and amplified using the QIAseq Small RNA Library Kit according to the manufacturer's instructions, followed by high-throughput sequencing on the Illumina platform.
SUB16358420
Ah-C1