Bulk RNA-seq of Mus musculus: female lung: SARS-CoV-2 virus challenge batch 2
Total RNA was extracted from mice lung formalin-fixed paraffin-embedded (FFPE) samples using the RNeasy FFPE kit (QIAGEN, catalog no. 73504), each with 4 sections of 10 m thickness. Extracted RNA was treated with the TURBO DNA-free reagent kit (Invitrogen, catalog no. AM1907) to remove contaminating DNA. The purified samples were checked for optimal quality on the Fragment Analyzer (Agilent, USA) for quality of DV200 at 47.8%~59.7%. Using 100 ng of DNAase-treated RNA, bulk RNA-seq libraries were prepared with the Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus kit (Illumina, catalog no. 20040525), and indexed with IDT for Illumina DNA/RNA UD indexes (Set A) (catalog no. 20027213), following manufacturers protocol. The cDNA libraries were amplified for 13 PCR cycles at the final PCR enrichment step.
SUB15390097
LTT23-EI03