Round 1: E. coli C
The amplified phage cocktail lysate  was extracted using the Qiagen DNeasy UltraClean Microbial Kit following the manufacturers protocol with the following modification. For step one, in a Powerbead tube, 300 mL of Powerbead Solution, 50 mL of Solution SL, and 280 mL phage lysate was added and heated at 70C for 10 minutes prior to disruption. DNA concentrations were quantified using a Qubit fluorometer. DNA was sent to SeqCenter (Pittsburgh, PA USA) for library preparation and sequencing. Samples were prepared for whole-genome sequencing using an Illumina DNA Prep kit and custom IDT 10 bp unique dual indexes. Sequencing was performed on the Illumina NovaSeq X Plus platform (San Diego, CA, USA) using a 300-cycle flow cell kit, producing a 2x150 bp paired end reads.  Demultiplexing, quality control, and adapter trimming was performed with Illuminas propriety software bcl-convert (v4.2.4) prior to delivery.
SUB16358095
D1_A