RNA-seq of JBMSCs proliferation and osteogenic differentiation
After total RNA extraction, mRNA was enriched using oligo dT magnetic beads.Following fragmentation of the mRNA, random primers were used for reverse transcription to synthesize the first strand of cDNA, with simultaneous introduction of strand-specific adapters.After synthesizing the second strand of cDNA, processes including end repair, A-tailing, sequencing adapter ligation, and PCR amplification were performed. Finally, a strand-specific cDNA library was constructed, which is suitable for paired-end sequencing on the Illumina sequencing platform.
SUB15799118
LRA260485