Metagenome of an acidophilic SRB enrichment
DNA extract from sample of day 160 was used to prepare metagenomic libraries using the Nextera XT DNA Library Preparation kit (Illumina, San Diego, CA, USA). Libraries were quality checked using an Agilent 2100 Bioanalyzer and DNA High Sensitivity kit and then pooled in an equimolar ratio. The pool was gel purified using a 2% agarose gel and the Qiagen QIAquick gel extraction kit (Qiagen, Germantown, MD, USA). Following purification, the pool was sequenced by Wright Labs, LLC (Huntingdon, Pennsylvania, USA) using an Illumina NextSeq to produce 2x150 bp reads.
SUB15569604
SA_F_R2