WGS of Pseudomonas aeruginosa: Canis lupus familiaris with External otitis
The cryopreserved isolates were plated on blood agar (Linsan, Santiago, Chile); and incubated at 37C. Genomic DNA was extracted using the fungal/bacterial Zimo kit (Zimo Research, Irvine, CA, USA) following the manufacturer's instructions. DNA purity was assessed spectrophotometrically with an Epoch Take 3 device (Bio Tek, CA, EEUU) using the 260/280 nm absorbance ratio. Sequencing libraries were prepared and submitted to SeqCenter (PA, USA), and whole genomes were sequenced on the Illumina MiSeq platform.
SUB15775863
3B_S3