Sea lice microbiome samples collected in four different months from an aquaculture facility in Ireland and sequenced in two different platforms (Illumina and ONT)
The 16S rRNA gene (region V3-V4) was amplified using 0.2 M of specific primers (341 F: 5-CCTAYGGGRBGCASCAG-3 and 806 R: 5-GGACTACNNGGGTATCTAAT-3) with the barcode with 5 L of Phusion High-Fidelity PCR Master Mix and 10 ng of template DNA. The initial denaturation step at 98 for 1 min was followed by 30 cycles of 98 for 10 s, 50 for 30 s, and 72 for 30 s and extension at 72 for 5 min . The samples were purified using magnetic bead purification . The samples were quantified, normalised, and then combined in equal proportions. Quantified and quality-checked libraries were pooled and sequenced according to the required data output.
SUB15807509
Lepeophtheirus_salmonis_metagenome_Illumina_February_4