DSS_GF_1
RNA samples were subjected to quality control on a 4200 Tapestation (Agilent), and concentrations were measured by the QUBIT fluorescent method (Life Technologies). For library construction, RNA input was normalized across all samples using a total RNA input of 250 ng (RIN >7). PolyA selection was performed using the reagents provided in the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, E7490L), and library construction was performed with the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs, E7760L) according to the manufacturer's user guide.
SUB15778210
10028