RNA-seq of EndoC H1 control
EndoC-H1 human pancreatic beta cells were cultured and treated with either 5.5 mM (control) or 16.7 mM (high glucose, glucotoxicity) glucose for 72 hours. Three independent biological replicates were collected per condition (total n = 6). Total RNA was extracted using the RNeasy Mini Kit (QIAGEN, Germany), and mRNA was enriched using Oligo(dT)-attached magnetic beads. RNA-seq libraries were prepared with the Illumina TruSeq Stranded mRNA Library Prep Kit and sequenced as paired-end reads (2  150 bp) on an Illumina NovaSeq 6000 platform.
SUB15737169
EndoC_control_3