Amplicon sequencing of Pyrus communis cv US200502_176
DNA from adult plants were extracted from leaf tissue using a Sbeadex Plant Magnetic Bead Kit. PCR was conducted to amplify genes located in two fire blight QTL regions on chromosome 2. Ion Torrent libraries were prepared for the bulked PCR reacions using the Ion Plus Fragment Library Kit and Xpress Barcode Adapter Kit. Libraries were loaded onto 540 chips using an Ion Chef system and a Ion GeneStudio S5 plus was used for sequencing.
SUB15903636
US200502_176_ASeq