Transcriptomic of Lt1 Cu10
The total RNA was extracted from 100 ml of culture using the SPINeasy RNA Kit for Yeast (MP Biochemicals), in accordance with the manufacturer's instructions. The RNA concentration and integrity were measured using a 2200 TapeStation (Agilent). The RNA samples (1 g) were dispatched to Macrogen (The Netherlands) for library construction and sequencing. The construction of cDNA libraries from total RNA was undertaken using the TruSeq Stranded mRNA Library Prep Kit. The quality of the libraries was then verified using a TapeStation D1000 Screen Tape. Subsequently, the process of sequencing was conducted on an Illumina NovaseqX platform, utilising a 10B flow cell. This facilitated the generation of paired-end reads, characterised by a length of 150 base pairs.Low-quality reads, adaptor sequences and other artefacts were removed, and the remaining clean reads were then aligned to the L. thermotolerans reference genome (CBS 6340) using the HISAT2 software. The absence of a comprehensive map for S. cerevisiae can be attributed to the statistical insufficiency of available data. The assembly and quantification of transcripts was conducted utilising the StringTie, with the expression profiles being reported as read counts.
SUB15439524
Lib_Lt1Cu10(1)