BCL mine sample 3 (distance from the mine 5 km)
the DNA libraries were prepared using the MGIEasy Universal DNA Library Prep Set (MGI Tech Co., Ltd., Shenzhen, China; Cat. Nos. 1000006985/6986/17571) following the manufacturers instructions. DNA was fragmented using a Covaris Focused-ultrasonicator (Thermo Fisher Scientific, USA), quantified with a Qubit 3 Fluorometer and dsDNA HS Assay Kit (Invitrogen, USA), and fragment size distribution was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies, USA). Library preparation included end repair, A-tailing, adapter ligation, bead cleanup, PCR amplification, and single-strand circularization.
SUB16223813
BCLSITE_3