hFXR_obesity_atac-seq-obese-OCA-1029
The goal of the experiment was to analyze the open chromatin on a genome wide scale in liver biopsy patient samples. The experiment was performed using the ATAC-seq kit from Active Motif. In the assay, intact nuclei were isolated following the Active Motif ATAC-seq protocol. These were the processed with a transposase according to the protocol that fragments and tags the accessible DNA. Libraries were PCR amplified, multiplexed and finally sequenced on a Nova-seq (Illumina).
Emilian Jungwirth
hFXR_obesity_atac-seq-obese-OCA-1029