16S rRNA amplicon from 0-10cm sediment sample S253, Three Gorges Reservoir
Adapters were ligated to the flanking regions of the target sequence by PCR, and the resulting PCR products were recovered by gel extraction using a gel recovery kit. The purified products were eluted with Tris-HCl buffer, and the eluate was detected by 2% agarose gel electrophoresis for quality control. Finally, the qualified products were denatured with sodium hydroxide to obtain single-stranded DNA fragments. The main reagent used in this process was the TruSeq DNA Sample Prep Kit.
SUB15981460
S253_LIB02