ITS seq Cheese: Fcheese6
DNA was extracted using the Qiagen DNeasy PowerFood Microbial kit and amplified using ITS1 (CTTGGTCATTTAGAGGAAGTAA) and ITS2 (GCTGCGTTCTTCATCGATGC) primers. The library was prepared using 2  Phanta Max Master Mix polymerase and final library was denatured, circularized, an amplified with phi29 polymerase to yield DNA nanoballs (DNBs), and the sequenced using the DNBSEQ-G400 platform
SUB15355613
F-Cheese6