Horizon HD829 Panel 6.25% dilution - Run 1
The VariantPlex Myeloid (Invitae, Colorado, USA) targeted NGS panel was used, which utilises anchored multiplex PCR for molecular barcoded, strand-specific library generation for 75 gene targets (S1 Table; total target size 125.4kb). Library preparation was undertaken per manufacturers instructions using 50-400ng of input DNA. Genomic DNA concentrations were determined using the Qubit dsDNA HS Assay Kit with the Qubit 3 Fluorometer (ThermoFisher Scientific, Massachusetts, USA). Individual library concentrations were quantified using the KAPA Library Quantification Kit (Kapa Biosystems) and libraries were pooled and requantified for sequencing. NGS was performed on Illumina (California, USA) NextSeq 500 and NovaSeq 6000 sequencers at a targeted depth of 3,000-5,000x.
SUB15031710
HD829_6.25dil_R1