WGS of E. coli isolates from pig feces in farms with frequent drug use
Genomic DNA libraries are prepared using the Nextera XT Library Prep Kit (Illumina,San Diego, USA) following the manufacturers protocol with the followingmodifications: input DNA is increased 2-fold, and PCR elongation time is increased to 45 seconds.DNA quantification and library preparation are carried out on a Hamilton MicrolabSTAR automated liquid handling system (Hamilton Bonaduz AG, Switzerland)
SUB16177127
D15