RNA-seq of Drosophila
Total RNA was extracted using a TRIzol kit (Invitrogen, USA), followed by quality assessment through three methods: agarose gel electrophoresis, NanoDrop micro spectrophotometry, and Agilent 2100 analysis. RNA fragments were enriched and then processed with the Hieff NGS Ultima Dual-mode mRNA Library Prep Kit for reverse transcription into cDNA. Gene Denovo Biotechnology Co.s Illumina was used to sequence the obtained cDNA library.
SUB15393418
m-1