Analysis of Genomic Adaptations of Pseudosulfitobacter pseudonitzischiae BPC-C4-2 from Antarctic Algae for the Establishment of a Cold-Water Bioassay System
A short-read sequencing library was prepared using a Nextera DNA Flex library preparation kit (Illumina, USA) and Nextera DNA CD indexes (Illumina) following the manufacturers protocol. A total of 1,826,456 paired-end reads (coverage 147.3) were generated using an iSeq 100 instrument (Illumina) on paired-end sequencing (2  150 bp). A long-read sequencing library was prepared with a one-dimensional ligation sequencing kit SQK-LSK109 (Oxford Nanopore Technologies [ONT]) and a NEBNext Companion Module for ONT Ligation Sequencing kit (New England BioLabs, USA) following each manufacturers protocol. No size selection or shearing was applied. The library was loaded into a FLO-MIN106 flow cell (R9.4.1, ONT) on a MinION sequencer (ONT). Fast5 reads were base then base-called using a MinKNOW v.19.10.1 (ONT) with Guppy v.3.3.3 in high-accuracy base-calling mode, generating 1,824,000 reads (coverage 269.3) with an N50 of 1,919 bp. FastQC v.0.11.7 [12] as employed to assess the sequence quality of both Illumina and ONT libraries.
SUB15184337
SQK-NBD114-24_barcode19