RNA-Seq-eggertii-daySIM
Leaves from different seedling lines were placed on SIM for 3 days. Total RNA was extracted using GeneJet Plant Total RNA kit (Thermo Fisher Scientific) with on-column DNase treatment (Millipore-Sigma) for removal of DNA contamination. Total RNA was quantified on NanoDrop Lite (Thermo Fisher Scientific), and the RNA integrity was verified on 1% agarose gel. Multiplexed cDNA libraries were produced and sequenced as previously described (Costa 2020, doi: 10.17504/protocols.io.bkypkxvn). Sequences were called, demultiplexed, and adaptor trimmed using MinKNOW v23.11.4.
SUB14788809
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