Whole genome sequencing of Microcystis flos-aquae NRMC-F 0457
Qualified DNA samples were taken for Illumina library preparation using TruSeq DNAPCRFree Library Prep Kit (Illumina, Inc., USA) where the genomic DNA is fragmented, endrepaired, A-tailed, size selected and ligated with Illumina sequencing barcoded adapters.Concentration of prepared library was checked using QuantStudio Real-Time PCR System(Applied Biosystems, USA). After dilution to 1 ng/ul, the Agilent 2100 bioanalyzer (AgilentTechnologies, Inc., USA) was used to assess the insert size. Sequencing was carried out at 2 150 bp in Illumina NovaSeq X platform (Illumina, Inc., USA)
SUB16326289
NRMC-F 0457