Atlantic salmon unaffected skin
Extracted RNA was subjected to library preparation using the Illumina Stranded mRNA Prep Kit (Illumina) according to the manufacturer's guidelines. Briefly, 100 ng RNA (or 25-100 ng for samples that did not yield sufficient RNA) was subjected to purification, fragmentation, and poly-A capture before first- and second-strand cDNA synthesis. For each sample, RNA index anchors (Mag-Bind TotalPure NGS, Omega Bio-tek) were ligated to the purified cDNA before library amplification with Illumina RNA UD Indexes. The final sequencing libraries were normalised and pooled before loading onto a NextSeq550 500/550 High-Output v2.5 (300 cycle) Flow Cell at a concentration of 1.3 pM to generate approximately 25 million read pairs per sample, according to the manufacturer's guidelines. Paired-end sequencing was completed at 2x 149 cycles with ten bp index reads
SUB14820098
21/050/01-12