16S rRNA gene sequencing of roots DNA from natural stands with Populus tremuloides: sample STFE.007.S.Roots
Libraries for sequencing were prepared according to Illuminas 16S Metagenomic Sequencing Library Preparation guide, with the exception of using Qiagen HotStar Taq DNA polymerase for the first PCR (amplicon PCR). The template specific primers were (without the overhang adapter sequence): 515F-5 (5- GTGYCAGCMGCCGCGGTAA-3) and 926R (5- CCGYCAATTYMTTTRAGTTT-3).
SUB15136754
034-STFE-007-S-Root-16S