RNA-Seq of mus musculus: IL-1beta and Senna-cotreated chondrocyte
Cultured primary mouse chondrocytes were incubated at 37C with 5% CO2 for 4 days. At 4 days, the cells treated with IL-1 (1 ng/mL) were co-treated with S. obtusifolia L. extract (100 ug/mL) for 24 hours and harvested on day 5. Total RNA was extracted from reagent-treated chondrocytes using TRIzol (MRC; TR-118) and chloroform (Sigma; 319988). The isolated total RNA was reversed transcribed, and cDNA was synthesized using the ImProm-II Reverse Transcriptase kit (Promega, A3803).
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