HiFi sequencing of Populus alba Villafranca
Young leaves of P.alba L. Villafranca, preserved in liquid nitrogen and stored at -80 C were used for the  extraction of  High-quality DNA using a modified CTAB protocol (Porebski et al., 1997). The quality of the extracted DNA was assessed through pulsed-field gel electrophoresis (CHEF) on 1% agarose gels to evaluate fragment size and restriction enzyme digestibility. Quantification was performed using Qubit fluorometry (Thermo Fisher Scientific, Waltham, MA). Sequencing was performed using PacBio Revio System at the Arizona Genomics Institute (Tucson, AZ), on Revio SMRT cells (PacBio Revio, 2024)
SUB15082962
P_alba_villafranca_HiFi_S3