Illumina HiSeq sequencing reads of Starmerella bombicola PYCC 5882
Genomic DNA was extracted from 1.5mL overnight cultures in YPD medium. Cells were harvested and resuspended in 500 l lysis buffer (10 mM Tris-HCl pH 8.0, 100 mM NaCl, 1 mM EDTA (pH 8.0), 2% Triton X-100, 1% SDS). An equal volume of phenol/chloroform (11 v/v) was added and the whole mixture was vigorously shaken on a Ika-Vibrax VXR shaker at 1800 rpm for 20 minutes, at room temperature and centrifuged at 14000 rpm for 10 minutes at 4C. Nucleic acids were precipitated with ethanol from the aqueous phase and were dissolved in TE buffer (10 mM TrisHCl, 1 mM ethylenediaminetetraacetic acid, pH 7.6) with RNase A (100 g/ml).Paired-end libraries were prepared from 1 g genomic DNA with an insert size of 300400 bp using the TruSeq DNA Prep Kit v2 (Illumina), following the manufacturers instructions. Sequencing was performed on an Illumina HiSeq 2000 platform for 2  100 cycles at the University of Wisconsin Biotechnology Center (WI, USA).
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Starmerella_bombicola_PYCC5882__Illumina.reads