Microbial Community Bacterial Cup100 Run 2 Rep 1
DNA extraction was performed on all digestates (both inocula and each digestate after each run). Similarly to Elboghdady et al., (2025), samples (40 ml) were pelleted at 13,000 rpm for 20 min. From 250 mg of each pellet, DNA was extracted using the DNeasy PowerSoil Kit (Qiagen, Germany) according to the manufacturers instructions. DNA yield and purity were quantified on a Nanodrop 1000 spectrophotometer (Thermo Fisher Scientific) while quality was determined through gel electrophoresis 1 % (w/v) 1  TAE agarose gels. DNA was stored at 80 C until analysis. The NGS was performed at BMR Genomics (Padova, Italy). Sequencing targeted the V3 and V4 regions of the bacterial 16S rRNA gene using primers 341F (CCTAYGGGRBGCASCAG) and 806R (GGACTACNNGGGTATCTAAT) (Yu et al., 2005) and V4 region of the Archaeal 16S rRNA gene using primers U519F (CAGYMGCCRCGGKAAHACC) and 806R GGACTACNSGGGTMTCTAAT) (Porat et al., 2010). The generated DNA libraries were sequenced with an Illumina NovaSeq PE284
SUB15985213
Bacterial Cup100 Run 2 Rep 1