Whole Genome Sequence of Mycobacterium Tuberculosis
The whole-genome libraries were prepared as per the manufacturers instructions. Briefly, the Ion Plus Fragment DNA Library Prep Kit (Thermofisher, Cat. No. 4471252) was used. Genomic DNA was first sheared to ~250 bp fragments using the Ion Shear Plus Kit (Thermofisher, Cat. No. 2664777), followed by adapter ligation and barcode tagging with the Ion Xpress Barcode and Adapters Kit (Thermofisher, Cat. No. 4471250). After each step, purification was performed using AMPure beads (Beckman Coulter, Cat. No. A63881). Library quality and concentration were assessed using a 2% E-gel (Size Select agarose gel) (Thermofisher, Cat. No. 2R300723-01).
SUB15809348
ZTB-44