H3K27me3 CUT and RUN on IPC purified from GW20 cerebral cortex
Radial glia (RG),  Intermediate progenitor cells (IPC) and Excitatory neurons (EN) were purified from human cortical brain tissue at gestational weeks (GW) 20 using flourescence activated nuclei sorting (FANS).  Sorted nuclei were incubated with Fab fragments to block antibodies used in FANS and ~25,000 nuclei were  subjected to CUT&RUN using  antibodies to H3K27me3 histone modification. Sequencing libraries were constructed using KAPA Hyperprep kit following manufacturer's instructions.  Libraries were sequenced using 150-bp paired-end sequencing on Illumina NovaSeq X Plus.
NIMH-phs000989
GW20_141_IPC_H3K27me3