The role of Klebsiella quasipneumoniae in rice straw degradation
Genomic DNA (100 ng) was fragmented to ~300 bp using a Bioruptor (Diagenode). Library preparation was performed using the NEBNext Ultra II DNA Library Prep Kit for Illumina following the manufacturers protocol. Fragmented DNA was end-repaired, A-tailed, and ligated with Illumina adapters. Index PCR was carried out to add sample-specific barcodes and enrich adapter-ligated fragments. Final libraries were assessed using a Fragment Analyzer (Agilent) and sequenced on an Illumina NovaSeq 6000 (2  150 bp).
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