Targeted COL4A3-COL4A6 amplicon sequencing of Alp52
Ultra-multiplex tiled amplicon PCR panel targeting all exonic regions of COL4A3, COL4A4, COL4A5 and COL4A6, designed with PCRpanel (seven pools; 474 primers / 237 primer pairs). Genomic DNA amplified by multiplex PCR (Phusion Hot Start II), pools combined, purified with AMPure XP, and sequenced paired-end on Illumina MiSeq (Reagent Kit v2 Nano, 500 cycles).
SUB16360170
Alp52-100