Removal of ibuprofen, diclofenac, and caffeine from synthetic sanitary sewage
Biomass samples from the UASB inoculum and from operational conditions 5, 7, and 10 were collected and stored at -20?C until further analysis. DNA extraction was performed using the FastDNA SPIN Kit for Soil (MP Biomedicals TM) following the manufacturers protocol. Microbial community characterization was conducted by the NGS facility "Solues Genmicas" (Piracicaba, So Paulo, Brazil) using the Illumina HiSeq PE250 platform. Primer set 341F/785R for Bacteria (Klindworth A et al. (2013)) and Ar915aF/Ar1386R for Archaea (Kittelmann S. et. al. (2013)) were used for PCR amplification. Sequencing data were processed following the workflow described by Callahan et al. (2016). Taxonomic assignment of amplicon sequence variants (ASVs) was performed using the DADA2 pipeline (Callahan et al., 2016b), with the SILVA 138 database. ASVs that could not be classified to at least the family level was excluded, and those assigned to the same genus were clustered. Taxonomic assignments and abundance data generated by DADA2 were then imported into the Phyloseq package (McMurdie et al., 2013) for downstream analysis.
SUB15528124
AnSBBR_Cond10 = Glucose bac