Whole exome sequencing of SHG142 glioblastoma cells (50th passage)
Genomic DNA was extracted from P50 SHG142 cells and subjected to quality con-trol. The qualified DNA was fragmented into 150350 bp fragments. Libraries were constructed via end-repair, A-tailing, and adapter ligation. Libraries were hybridized with biotinylated exon capture probes (Illumina) and enriched with streptavidin-coated beads. The captured DNA was PCR amplified. The final libraries were quantified and sequenced on an Illumina NovaSeq 6000 platform (PE150 configuration).
SUB15451658
SHG142_WES_P50