DAP seq of CP23001343-GhMYB44-1-GhMYB44-1
DAP-seq was performed for GhMYB44/GhMYB73 using purified genomic DNA (~200 bp fragments) incubated with HaloTag-fused GhMYB44 protein expressed in a wheat germ system. Bound DNA was recovered, PCR-amplified, and sequenced on the Illumina NovaSeq platform. Clean reads were aligned to the Gossypium hirsutum CRI v1.0 genome using BWA-MEM, and peaks were annotated with ChIPseeker.
SUB15740220
F3