Whole genome sequencing of Dellaglioa kimchii P0083: kimchi isolate_Illumina
Genomic DNA from isolates was extracted using the Qiagen MagAttract HMW DNA kit (Qiagen, Hilden, Germany), in accordance with the manufacturers instructions. The concentration of extracted DNA was quantified using the Qubit 2.0 fluorometer (Invitrogen, Carlsbad, USA). Contamination of DNA or cultured strain was checked by sequencing the 16S rRNA gene using the ABI 3730 DNA sequencing machine (Applied Biosystems, Foster City, CA, USA). The specification of DNA sample quantity and quality are below.To generate libraries, genomic DNA was fragmented to approximately 550 bp using the M220 Focused-ultrasonicatorTM (Covaris Ltd, Brighton, UK) and fragmented DNA was quantified using the Bioanalyzer 2100 (Agilent, Palo Alto, USA) with the DNA 7500 kit. The library was constructed using the TruSeq DNA Library LT kit (Illumina, San Diego, USA) according to the manufactures protocols. The whole-genome sequencing was performed on the Illumina MiSeq system with 2 x 300 bp paired-end reads using the 600 cycle (MiSeq Reagent Kit v3) sequencing kit.
SUB14866233
P0083_WGS_Illumina