RNA-seq of the Brain of Notacanthus bonaparte
For each sample, total RNA extraction was performed using NZYol for extraction and NZY Total RNA Isolation kit with a DNase I on-column step for purification (NZYTech, Lda. - Genes and Enzymes), following the manufacturers instructions. RNA concentrations (ng/L) were measured using a DS-11 Series Spectrophotometer/Fluorometer. The total RNA was sent to Macrogen Inc. for Illumina paired-end strand-specific libraries (insert size of 250300 bp) preparation and sequencing of 150 bp paired-end reads using a HiSeq4000 platform.
SUB14660010
P22_301B