DNA-Seq of Capreolus pygargus: skeleton remain
The tarsal bone from ancient Siberian roe deer was deposited at Jilin University (www.jlu.edu.cn, contact person: Dawei Cai, Email: caidw@jlu.edu.cn) and was labeled with archaeological ID 22CNSXF1SD:3 and lab code XTS03G. In the sample preparation stage, an electric grinding device and sterile disposable drill bits were employed to abrade the 2-mm outer cortical layer of the bone. Subsequent to cleaning, the specimens were subjected to treatment with a 10% chloramine solution, decontamination using DEPC water, and a 5-minute soaking in 70% ethanol, followed by drying under ultraviolet (UV) light irradiation. On the subsequent day, the processed specimen was ground into a fine powder using an electric grinder, and 100 milligrams of powder was collected from each sample. The ancient DNA extraction procedure adopted in this research is in accordance with the protocol outlined by Dabney et al. (2013), which involves the use of the MinEluteVR PCR Purification Kit for solution-based extraction. For DNA library construction, the experimental method established by the Max Planck Institute (Germany) was modified and optimized (Yates et al. 2021). After purification of the DNA libraries, paired-end sequencing (read length: 150 bp) was carried out on an Illumina Hiseq X sequencing platform by Novogene Inc., located in Beijing, China.
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XTS03G