hybrid capture respiratory
Double-stranded cDNA was tagmented using Illumina DNA Prep small bead link transposase system and amplified using a custom primer to add on ONT PCR adapter sequence. Libraries were enriched for viral nucleic acid using the Illumina DNA Prep with Enrichment Kit hybridization reagents with Twist Comprehensive Viral Research Panel probes, followed by MinION barcoding, loading and sequencing. Average final library size is approximately 300 nt. Protocol link: https://www.protocols.io/view/pathoseq-bio-hybrid-capture-with-ont-minion-sequen-36wgqd5eovk5/v160
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