Whole genome sequencing of Clostridium perfringens: Isolate from a patient stool in a food poisining outbreak
DNA extraction was performed on C. perfringens isolates grown under anaerobic conditions (16 hours at 37?C in BHI medium). The bacteria were washed and treated with lysozyme (1 hour at 37?C), suspended in lysis buffer and the nucleic acids were extracted using magLEAD 12gC device (Precision System Science Co.). The sequencing libraries were prepared by Tagmentation using the Illumina DNA Prep kit (cat #20060059), with IDT for Illumina DNA/RNA UD Indexes Set B (cat # 20027214). The resulting libraries were sequenced on a MiSeq instrument using the MiSeq Reagent Kit v2 (500-cycles, cat #MS-102-2003).
SUB15451006
990-b_S13