Unaltered
Bulk RNA-seq was conducted on RNA extracted from frozen tissue samples. RNA was DNase-treated, rRNA-depleted (Qiagen FastSelect), and libraries prepared using the NEBNext Ultra II RNA Library Prep Kit, followed by Illumina NovaSeq sequencing (2×150 bp paired-end); data were base-called and demultiplexed using Illumina software (bcl2fastq).
NCI-phs004424
B2-BB-202107