RNA-seq of leaves: Caragana korshinskii
Total RNA from C. korshinskii was extracted using the TRIzol reagent kit. Residual genomic DNA was removed by treating the RNA with DNase I at 37 C for 30 min. RNA integrity was assessed on an Agilent 2100 Bioanalyzer, ensuring RNA integrity number (RIN) values  6.5. Firststrand cDNA synthesis was performed using the BGI UMI base PCR cDNA Synthesis Kit. cDNA molecules longer than 4 kb were PCRamplified to generate secondstrand cDNA, and the resulting doublestranded DNA was subjected to a second round of PCR amplification for SMRTbell library preparation. Libraries were sequenced on the PacBio Sequel platform. Raw reads were assembled with the SMRT Analysis v5.0.1 suite and polished using the Quiver algorithm.
SUB15489219
Caragana korshinskii 1