Metagenome sequence of MCW2 soil
The PacBio SMRTbell library was prepared for sequencing from the concatenated amplicons following manufacturers protocol.  In this protocol, DNA fragments were treated with DNA damage repair mix, end-repaired, and 5 phosphorylated. PacBio hairpin adapters were ligated to the fragments to create SMRTbell templates for sequencing. The SMRTbell templates were purified using exonuclease treatments and size-selected using the Sage Science BluePippin instrument with a 10 kb lower cutoff depending on DNA quality
SUB15656196
M13_bc1006_F--M13_bc1059_R