Genome-wide integration mapping of HEK293FTs:Dn29-dCas9 transfection with attP donor and H1-g2, replicate 2
gDNA is extracted from edited cells by Zymo Quick DNA miniprep. DNA is randomly tagmented with Tn5 to introduce adapters. Nested PCR is performed to amplify the junction between the integrated plasmid donor and the genomic integration locus by amplifying the plasmid and the Tn5 adapter.
SUB14788129
dCas9Dn29_C5