metagenomic shotgun sequencing of human feces: IBD Patient 5, 0h, NSC Treatment
The DNA was extracted using Tiangen Magnetic Soil And Stool DNA Kit. Library preparation was carried out using the Novogene NGS DNA Library Prep Set (Cat No.PT004) with index codes assigned to each sample. Briefly, genomic DNA was randomly fragmented to size of 350bp. The DNA fragments were end-polished, A-tailed, ligated with adapters. Size selection was performed, followed by Rolling Circle Amplification. The resulting PCR products were purified using the AMPure XP system and analysed for size distribution using an Agilent 2100 Bioanalyzer (Agilent Technologies, CA, USA). Quantification was performed using real-time PCR. The library was then sequenced using the Novaseq X plus flow cell with PE150 strategy.
SUB14990566
P132_E01_5