high methylation control
Genomic DNA from human whole blood was enzymatically treated to achieve high or low methylation levels (purchased from EpigenDX). Standard nanopore sequencing libraries were prepared using the ligation kit - SQK-LSK110 following the manufacturer's instructions. The resulting library was then loaded into a MinION R9.4.1 flow cell and sequenced on the MinION device. Using the MinKNOW software, an adaptive sampling run (set to enrich) was performed with parameters set to use the "fast" basecallilng model of Guppy. Target regions were defined as a FASTA file containing target sequences.
SUB15151273
high methylation control