PacBio Iso-Seq long-read sequencing of rRNA-depleted RNA from amputated WT S. mediterranea at 0 hpa
Wild-type S. mediterranea worms were amputated and collected immediately. Total RNA was ribodepleted and used for library preparation using Iso-Seq express 2.0 kit and Iso-Seq SMRTbell prep kit 3.0. The library was sequenced using Sequel IIe.
SUB15224508
PacBio Iso-Seq (rRNA-depleted RNA, amputated WT, 0 hpa)