Pooled PD CD4 T-cells (TCR)
Single-cell RNA and TCR sequencing were performed using the 10x Genomics Single Cell 5 Reagent Kits (v3 4-plex OCM) with immune receptor mapping, capturing 10,000 cells per sample and generating 20,000 GEMs, followed by cDNA recovery, amplification (12 PCR cycles), SPRIselect cleanup, and library construction for GEX and V(D)J TCR libraries; libraries were quantified by Bioanalyzer and qPCR, normalized to 2 nM, denatured with NaOH, loaded at 130 pM on an Illumina NovaSeq X Plus (Read1: 28 cycles, Read2: 90, Index1/2: 10 each), sequenced to ~50,000 reads per cell, and processed with Cell Ranger multi v9.0.0 for mapping and quantification.
SUB15674644
SUB15568559-TCR