RNA-seq_A16-66-20_UM20_buffy_coat
Patients with prostate cancer underwent radical prostatectomy with no prior therapy. Blood was drawn into EDTA tubes prior to surgery. RNA was extracted from frozen buffy coats using the Macharay-Nagel NucleoSpin RNA Blood kit. Libraries were prepared with the NEBNext Globin and rRNA depletion library construction kit and the Illumina TruSeq stranded total RNA with ribo-zero globin kit.
NCI-phs001813
A16-66-20