Metabarcoding of fungi: Fagus sylvatica decaying bark
Amplicons were normalized to 200 fmol, assuming an average amplicon size of 700 bp, and barcoded using the Native Barcoding kit 96 V14 (SQK-NBD114.96). 25 fmol was loaded on an R10.4.1 MinION flowcell. Sequencing ran for 72 hours at 260 bp/s.
SUB14681984
barcode29