DAP-seq control (Arabidopsis thaliana) with library A/15 cycles
Genomic DNA was isolated from a 4-week-old Arabidopsis thaliana plant in two replicates (A and B) using a Nucleobond HMW DNA kit (Macherey-Nagel). The amplified DAP libraries were prepared using an adapted Galli et al. (2018) protocol. DAP-seq was then performed for the HY5 (AT5G11260) transcription factor and a HaloTag control using a modified protocol based on Bartlett et al. (2017). After recovering the bound DNA, we implemented a crucial quantification step to determine the optimal number of amplification cycles. For the comparative analysis, a fixed 15-cycle amplification was also run on HY5 and the control. The resulting DNA libraries were sequenced on the NextSeq 2000.
SUB15735171
AT_control_libA_15cycles