PacBio AMF amplicon of maize roots
AMF communities were characterised by sequencing a long rDNA fragment spanning the SSUITSLSU regions using a modified two-step PCR protocol based on Kolakov et al. (2021) and modified as described in Barmettler et al (2026). In brief, two primer pairs (NS31/LSUmAr and AML1/LSUmAr) were used in PCR1, followed by PCR2 with NS31_Glo3/LSUmBr primers incorporating M13 tails and a 5AmMC6 modification to prevent carry-over ligation. PCR1 products were pooled and amplified in triplicate during PCR2, purified using SPRI beads, and subsequently indexed with custom M13 barcodes. Size-selected amplicons (15003000 bp) were gel-purified, quantified, pooled, and sequenced on a PacBio Revio platform (SMRT Cell 25M). Library preparation and sequencing were performed at the Functional Genomics Centre Zurich.
SUB15909318
Gras3_MAX_R2