16S rRNA gene amplicon sequencing of mouse fecal microbiota sample DSS01
Genomic DNA was extracted from mouse fecal samples using the E.Z.N.A. Mag-Bind Soil DNA Kit. The V3-V4 region of the bacterial 16S rRNA gene was amplified using barcoded primers 338F (5'-ACTCCTACGGGAGGCAGCA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3'). Amplicon libraries were prepared using the NEBNext Ultra DNA Library Prep Kit for Illumina and sequenced on an Illumina NovaSeq 6000 platform using paired-end 250-bp sequencing.
SUB16357463
DSS01_16S_V3V4