Hi-C sequencing for chromosome-scale genome assembly
In short, the library was constructed through the following steps: crosslinking cells with formaldehyde, digesting DNA with a  suitable 4-cutter restriction enzyme MboI, filling ends and mark with biotin, ligating the resulting blunt-end fragments, purification and random shearing DNA into 300-500 bp fragments. After quality control test of the libraries using Qubit 2.0, an Agilent 2100 instrument (Agilent Technologies, CA, USA) and q-PCR, 150 bp PE sequencing of the Hi-C library were performed on the Illumina Novaseq X plus platform.
SUB16025738
Muscle_DNA_MGI_HiC_Lib1