CITE-seq of PBMCs of AS pt 232, GEX (set 9 of 12)
Whole blood was collected into EDTA tubes from patients newly diagnosed with prostate cancer. PBMCs were purified from whole blood using the MACSprep PMBC Isolation kit. Cell viability was assessed using a Luna FX7. For CITE-seq captures, 1M PBMCs were stained with BioLegend TotalSeq-C Human Universal Cocktail of antibodies. The cellular samples were loaded in the lane according to the 10X Genomics Chromium Next GEM Single Cell 5'v2 (Dual Index) User Guide with a single capture lane per sample. Reverse transcription PCR was run overnight. All subsequent steps of library preparation and quality control were performed as described in the 10X user guide.
NCI-phs001813
SCAF4550_GEX_S24_L007