RNA-seq of lily: JB
Total RNA was extracted from leaf samples of KR, JB, and JN using the GeneAll RiboSpin Plant reagent. RNA concentration was confirmed using the Quant-it RiboGreen RNA Assay kit (Invitrogen, cat. #R11490), and the integrity of the total RNA was assessed using the TapeStation RNA ScreenTape (Agilent, #5067-5576). For library construction, 1ug of total RNA was used to isolate highly purified mRNA which was sheared briefly into mRNA fragments using oligo (dT) beads. These sheared mRNA fragments were reverse transcribed into first-strand cDNA using SuperScript II reverse transcriptase with random primers (Invitrogen, #18064014). Next, the purified cDNA was end-repaired and affixed with poly(A) tails and the processed cDNAs were amplified with PCR to construct the cDNAs library
SUB16325936
JB