RNA-Seq of S.melongena, leafs
Sample of leaf material eggplant cultivar group Almaz (Alm). Leafs of approximately 20 plants were collected in a fixative buffer. Isolation of total RNA was performed using TRIzol, yielding two tubes of RNA preparations per sample, which were dissolved in nuclease-free water (NFW, Thermo Fisher Scientific, USA). RNA samples were treated with DNase I (RNase-free, 50 U/L, Thermo #EN0523) at a ratio of 1 unit per 1 g of RNA. 1 L of RiboCare RNase Inhibitor was also added to each sample (40 e.a./L, Eurogen, EK005S). The mixture was incubated at 37C for 30 minutes before being resuspended with TRIzol-chloroform without temperature termination. Total RNA libraries were constructed using TruSeq Stranded Total RNA with Ribo-Zero (Illumina) kit, which enables rRNA depletion.
SUB14595074
N32-Alm