metatranscriptomics from anode biolfilm
First, the ribosomal RNAs from both eukaryotes and prokaryotes were depleted from total RNA samples. The remained RNAs were fragmented into around 250~300 bp and then reverse-transcribed into double-stranded cDNAs which subsequently went through end repair, A tailing and adapter ligation. After fragments size selection and PCR amplification, the metatranscriptome library was ready for library QC and sequencing.
SUB14653141
R15R