Hi-C of phodopus sungorus female liver
chromatin was fixed in place with formaldehyde in the nucleus and then extracted. Fixed chromatin was digested with DpnII, the 5 overhangs filled with biotinylated nucleotides, and free blunt ends ligated. After ligation, cross-linking was reversed and DNA was purified. Following purification, biotin not contained in the center of the ligated fragment was removed. DNA was sheared to 350 bp mean size and libraries were generated using Illumina-compatible adaptors.
SUB14992324
Hi-C_rep2