scRNA-Seq for human melanoma
Isolated cellular suspensions were prepared and subsequently loaded onto the 10x Chromium Single Cell Controller. This innovative system utilizes microfluidics technology to encapsulate each individual cell with a uniquely barcoded gel bead within nanoliter-scale droplets, producing gel bead-in-emulsions (GEMs). Following this, the scRNA-seq libraries were constructed by employing the Chromium Single Cell 5 Library & Gel Bead Kit provided by 10X Genomics. Once the library preparation was completed, the fully constructed libraries were loaded onto an Illumina HiSeq X Ten sequencing platform with a 150-bp paired-end read configuration. This process was executed in strict adherence to the manufacturers recommended protocol.
SUB14853587
R14