chickens gut viral metagenome
Total RNA was removed ribosomal RNA, and the obtained mRNA was randomly broken into short fragments. The first strand of cDNA was synthesized with random hexamers, and then the second strand cDNA was synthesized with dNTPs, RNase H, DNA polymerase I and buffer. The cDNA was purified by QiaQuick PCR kit and eluted with EB buffer, followed by end repair, base A and sequencing adapter, and then recovered by agarose gel electrophoresis and PCR amplification.
SUB15019557
Adult15