PacBio Transcriptome Sequencing of Bathyraja eatonii
Using the SMARTer PCR cDNA Synthesis Kit (Clontech, Palo Alto, CA, USA), RNA is synthesized to cDNA. The SMRTbell libraries were subsequently prepared using the SMRTbell Template Prep Kit 1.0-SPv3. The sequencing was performed on the SMRT cells using the Sequel Sequencing Kit 2.0. The high-quality Iso-Seq reads were extracted using SMRTLink v8.0.
SUB16120064
Fish17_Isoseq