RNA-Seq of sourdought: spontaneous 60 h
DNA from microbiome samples was isolated using the Monarch HMW DNA Extraction Kit for Tissue (New England Biolabs, USA). DNA amplification was performed with specific primers to the conserved 16S site of the microorganism. The libraries were indexed using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs, USA). Sequencing was performed on a MiSeq sequencer with a set of MiSeq v.3 reagents (600 cycles).
SUB16259688
S2_spont_60h_16S