TargetSeq_Sample_SCD_TDT_61:P1,3M,NK cell,HBG1/2-ON
PrimeSTAR HS DNA Polymerase was used to amplify the sequence of target site from the genomic DNA. PCR product was gel purified using FastPure Gel DNA Extraction Mini Kit. DNA libraries were prepared following the manufacturers instructions, with kit selection based on amplicon length: NEBNext Ultra II FS DNA Library Prep Kit (NEB) for long fragments (>300 bp); KAPA Hyper Prep Kit for short fragments (300 bp).
SUB16354305
TargetSeq_Sample_SCD_TDT_61