6-base sequencing of Buffy coat DNA from patient 2189, Progression
Blood was drawn into Streck tubes at each timepoint. Blood was centrifuged, plasma was isolated and centrifuged a second time, and buffy coat and plasma were isolated and frozen. gDNA was extracted from buffy coat using the Qiagen DNeasy Blood and Tissue Kit. DNA was assembled into 6-base libraries using the evoC biomodal library prep kit.
NCI-phs001813
15-c-0145_033_EOT-BC