WGS of Manis culionensis: blood
Libraries were prepared by the Centre for PanorOmic Sciences Genomics Core at The University of Hong Kong. Briefly, 100ng DNA was sheared to 300bp with the Diagenode Biorupter Pico system, then used to prepare libraries following the KAPA HyperPrep Kit protocol, including end-repair, A-tailing at the 3' end, and adaptor ligation with an IDT dual-indexed UMI adaptor system. Libraries were selected for size range of 300-750bp with the dual-SPRI method and enriched with PCR. Libraries were then sequenced for 150bp paired-end reads.
SUB15419388
PPAE001