18S rRNA gene sequencing of greenhouse soil DNA from pots with Populus tremuloides seedlings: sample SP-56.Soil
Libraries for sequencing were prepared according to Illuminas 16S Metagenomic Sequencing Library Preparation guide, with the exception of using Qiagen HotStar Taq DNA polymerase for the first PCR (amplicon PCR). DNA was first amplified using primers AML1 (5-ATCAACTTTCGATGGTAGGATAGA-3) and AML2 (5-GAACCCAAACACTTTGGTTTCC-3), targeting the V3-V4-V5 region of the 18S rRNA gene. The resulting PCR products were then used as a template in a second PCR reaction with primers nu-SSU-0595-5 (5-CGGTAATTCCAGCTCCAATAG-3) and nu-SSU-0948-3 (5-TTGATTAATGAAAACATCCTTGGC-3), targeting the V4 region of the 18S rRNA gene.
SUB15136754
18S-AMF-sol-SP-56