RNA-seq of MEAM1 Bemisia tabaci: adult treated at 27 degree
After total RNA was extracted, mRNA was purified using Dynabeads Oligo (dT) with two rounds of purification and then was fragmented into short fragments. The cleaved RNA fragments were reverse-transcribed to create the cDNA by SuperScriptII Reverse Transcriptase. Dual-index adapters were ligated to the fragments, and size selection was performed with AMPureXP beads. The ligated products were amplified with PCR.
SUB14626429
CK-27-1