Pool-seq Sardina pilchardus: English Channel_ENC_J
Genomic DNA was extracted from all sardines using the DNeasy Blood &amp; Tissue Kit (Qiagen), following the manufacturers instructions, including the RNase A treatment. For each sample collection (n = 34), DNA from 25 individuals was pooled in equal amounts and quantified. Pool-seq library construction and whole-genome shotgun sequencing (NovaSeq6000, Illumina) were performed by Macrogen (Seoul, Republic of Korea), generating 2x150 bp paired-end reads from 350-bp insert libraries (TrueSeq DNA Nano, Illumina) with a minimum coverage of 60x based on a genome size of 1,100 Mb.
SUB15020567
Pool24