16S rRNA sequencing of microbial structure
The universal primers were chosen. Using the diluted genomic DNA as a template, PCR amplification was performed with the high-fidelity enzyme 2Phanta Flash Master Mix (Dye Plus) PCR kit to ensure the accuracy and efficiency of amplification. The PCR products were monitored on a 11% agarose gel. Subsequently, the sequencing of the PCR products was conducted on the Oxford Nanopore platform. Finally, the raw data were subjected to a quality control procedure.
SUB15083113
SLDP-5