Atlantic salmon skin with mild red skin disease
Extracted RNA was subjected to library preparation using the Illumina Stranded mRNA Prep Kit (Illumina) according to the manufacturer's guidelines. Briefly, 100 ng RNA (or 25-100 ng for samples that did not yield sufficient RNA) was subjected to purification, fragmentation, and poly-A capture before first- and second-strand cDNA synthesis. For each sample, RNA index anchors (Mag-Bind TotalPure NGS, Omega Bio-tek) were ligated to the purified cDNA before library amplification with Illumina RNA UD Indexes. The final sequencing libraries were normalised and pooled before loading onto a NextSeq550 500/550 High-Output v2.5 (300 cycle) Flow Cell at a concentration of 1.3 pM to generate approximately 25 million read pairs per sample, according to the manufacturer's guidelines. Paired-end sequencing was completed at 2x 149 cycles with ten bp index reads
SUB14820098
21/050/01-10