Enterococcus durans ED01 isolated from artisanal cheese
Genomic DNA libraries are prepared using the Nextera XT Library Prep Kit (Illumina, San Diego, USA) following the manufacturers protocol with the following modifications: input DNA is increased 2-fold, and PCR elongation time is increased to 45 seconds. DNA quantification and library preparation are carried out on a Hamilton MicrolabSTAR automated liquid handling system (Hamilton Bonaduz AG, Switzerland). Libraries are sequenced on an lllumina NovaSe 6000 (Illumina, San Diego, USA) using a 250 bp paired end protocol. For projects predating April, 2020, libraries were sequenced on an Illumina HiSeq 2500 using a 250 bp paired end protocol (Illumina, San Diego, USA). Reads are adapter trimmed using Trimmomatic version 0.30  with a sliding window quality cutoff of Q15. De novo assembly is performed on samples using SPAdes version3.7, and contigs are annotated using Prokka 1.11
SUB15735370
294726