Moesziomyces globuligerus strain:NYNU 178249 Genome sequencing and assembly
Genomic DNA of Moesziomyces globuligerus strain NYNU 178249 was extracted from cell biomass using a CTAB-based protocol. High-molecular-weight DNA was sheared into long fragments suitable for SMRTbell library construction, followed by end-repair and ligation of SMRTbell adapters according to the manufacturers instructions (PacBio). The library was treated with exonucleases to remove incompletely ligated products and purified. Size selection was performed using a BluePippin system to enrich long DNA fragments. Sequencing was carried out on a PacBio Sequel II platform to generate long-read data for de novo genome assembly.
SUB15809472
Mg_PacBio_WGS