RNA-seq of 0012 Tumor
FFPE human prostate tissues were sectioned onto glass slides, and regions of interest were macrodissected. Total RNA was extracted from macrodissected tissue using the Qiagen RNeasy FFPE kit. rRNA-depleted RNA was converted into cDNA and assembled into strand-specific libraries using the NEBNext Ultra II Directional RNA-Seq kit.
NCI-phs001813
A24-3-21