PacBio HiFi WGS of Chionea alexandriana DNA Zoo Sample2747
PacBio HiFi data was generated from a single Chionea alexandriana individual collected in the Olympic mountain range in Seattle, Washington, USA (species identity determined via DNA 49 barcoding) at Mount Sinai School of Medicine. Specifically, CryoPrep was used for homogenization, followed by MagAttract (Qiagen) for HMW gDNA extraction. The SMRTbell library was prepared from 5 g of sheared genomic DNA using the SMRTbell Express Template Prep Kit 2.0, followed by size selection using the SageELF system. The library was sequenced on PacBio Sequel II system using a single SMRT cell with v2 chemistry and 30-hour movie time to generate high-fidelity (HiFi) reads.
SUB15525654
PBG-2731