RNAseq of Gammarus pulex: Sweden
Gammarus pulex were collected from 13 sites in southern Sweden (agricultural and urban areas near Malm-Lund) and central Germany (modified river systems with agriculture, WWTPs, and urbanization) in summer 2022. At each site, 20 habitat-weighted samples (1 m) were taken using a Surber sampler (500 m mesh), and specimens were stored in a cryoshipper until RNA extraction from 65 individuals (5 per site) from five rivers/streams in Skne, Sweden, and the River Holtemme, Germany. RNA was extracted using the RNeasy Plus Mini Kit (Qiagen) with DNase I treatment to remove genomic DNA, and quality was assessed via Qubit, NanoDrop, and TapeStation (RIN>7). Library preparation (Novogene, UK) involved mRNA purification, fragmentation, cDNA synthesis, and amplification, with validation via Qubit 2.0, real-time PCR, and BioAnalyzer. Sequencing was performed on an Illumina NovaSeq 6000 with 150 bp paired-end reads.
SUB15106623
SW_25