Bos taurus liver RNAseq C1836
Total RNA was extracted from bovine liver tissue. mRNA was isolated using poly-T oligo-attached magnetic beads and fragmented prior to first- and second-strand cDNA synthesis. Libraries were prepared using end repair, A-tailing, adapter ligation, size selection, PCR amplification, and purification. Library quality and concentration were assessed using Qubit, qPCR, and Bioanalyzer. Sequencing was performed on an Illumina NovaSeq 6000 platform to generate paired-end reads for transcriptome analysis.
SUB16002028
C1836