Fugal amplicons corresponding to the area not affected by yellow stain
For fungi, PCR amplification targeted the ITS2 region of fungal nuclear ribosomal DNA using specific primers. DNA amplicon libraries were generated by PCR, followed by the addition of Illumina sequencing adapters and dual-index barcodes. The libraries were then normalised, pooled and loaded onto a MiSeq Reagent Cartridge spiked with PhiX control to improve base calling during sequencing.
SUB14653613
CSNC_ITS