AM-2-1:ITS sequencing of surface fungi from Astragalus slices
Paired-end 16S rRNA (V3-V4) and ITS amplicon sequencing was performed on Illumina NextSeq 2000 to profile bacterial and fungal communities on Astragali Radix surfaces. Libraries were constructed from genomic DNA extracted from surface samples, amplified with barcoded primers (338F/806R for 16S, ITS1F/ITS4R for ITS), and sequenced (2150 bp). Raw data were processed into FASTQ format for subsequent microbiome analysis.
SUB16064704
AM-2-1_ITS_Lib