Shock-Control
We extracted total RNA from flash frozen copepods using a combination of TRIzol reagent (Invitrogen; catalog no. 15596026) and the Qiagen RNAeasy Plus kit (Qiagen, catalog no. 74134). Briefly, copepods were homogenized using a TissueRuptor II (Qiagen, catalog no. 9002755) before we followed steps 1-7 of the TRIzol protocol, followed by steps 4-11 of RNAeasy Plus kit protocol. Total RNA extracted was sent to Novogene Corporation Inc. at Sacramento, California, where RNA quality control was confirmed using a 2100 Agilent Bioanalyzer on a Eukaryote Total RNA Nano chip and nondirectional libraries were produced using polyA tail selection. The resulting 60 libraries were sequenced on NovaSeq X Plus, with 150bp paired-end reads.
SUB15620879
NB4