Small RNA-Seq of Danio rerio: whole embryo at 16 hpf
Total RNA was extracted from zebrafish embryos or FACS-sorted cell populations using the Qiagen miRNeasy Mini Kit, following the manufacturers instructions. Neural crest and non-neural crest cells were isolated by fluorescence-activated cell sorting (FACS) from Tg(sox10:eGFP,mRFP) embryos at either 16 or 28 hours post-fertilization (hpf). RNA quality was assessed by Bioanalyzer (Agilent), and small RNA libraries were prepared from 200 ng of total RNA using the Illumina TruSeq Small RNA Library Preparation Kit. Libraries were size-selected, purified, and pooled prior to sequencing on an Illumina NextSeq 500 platform using a High Output single-end 75 bp configuration.
SUB15395219
SRALIB01