PacBio HiFi sequencing for genome assembly
Genomic DNA (gDNA)was extracted from the muscle tissue of Hemibarbus labeo according to the instruction of Blood & Cell Culture DNA Kit (Qiagen, 13323) . DNA quality was analyzed using NanoDrop (Thermo Fischer Scientific, ND2000), QubitInvitrogen, Q33238, and electrophoresis.After examination of the quality of isolated DNA, the library was constructed using a SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences, CA, USA). The construction includes DNA shearing, AMPure PB Bead purification, ssDNA overhangs removing, damage repair, end repair, hairpin adapter ligation, and bead purification of the library. After quality control test, a SMRT bell library was obtained. The library was sequenced on PacBio platform (Pacific Biosciences, CA, USA). HiFi reads were generated by SMRTLink.
SUB16040362
Muscle_DNA_PacBio_HiFi_Lib1