16S RNA sequence
A dual-barcoded 2-step PCR reaction was conducted to amplify a fragment of the V3eV4 hypervariable region of the bacterial 16S ribosomal RNA (rRNA) gene. Equimolar concentrations of the universal primers S-D-Bact-0341-b-S-17 ACACTGACGACATGGTTCTACACCTACGGGNGGCWGCAG) and S-D-Bact-0785-a-A-21 (TACGGTAGCAGAGACTTGGTCTGACTACHVGGGTATCTAATCC) were used as previously described (Klindworth et al., 2013)
SUB15234221
MP14