Whole Genome Resequencing of Schizosaccharomyces pombe strain ZB4421
Approximately between 1 and 4 g of genomic DNA was first sheared to a target size of around 150bp in a microTUBE AFA fiber pre-slit snap-cap Covaris tube in the Covaris M220 focused ultrasonicator (150W Peak Incident Power, 10% duty Cycle, 200 cycles per burst, 430s total time). The DNA was then purified with 1.8 volumes of MagBio HighPrep magnetic beads. 1g of sheared DNA were used to prepare barcoded libraries using the NEB ultra II DNA library prep kit, pooled and sequenced in a NextSeq 500 2x75 flow cell.
SUB15527958
B4