ONT RNA-Seq data of Melinaea mothone: Forewing imaginal disc extracted 48h after pupation
RNA-seq nanopore long reads were generated from forewing and hindwing disc tissues (Day 2 after pupation). Wing discs were dissected out and either flash-frozen (Mec. messenoides and Mel. mothone) or stored in RNAlater (Mel. menophilus) at -70C until processing. Following extraction, RNA quality was assessed using the Agilent Bioanalyzer. Oxford Nanopore Technologies full-length cDNA sequencing libraries were prepared using the ONT cDNA-PCR Sequencing V14 Barcoding kit (SQK-PCB114.24). Barcoded libraries, with cDNA pooled at equimolar concentrations, were sequenced on R10 flow cells (FLO-PRO114) using an ONT PromethION sequencer running MinKNOW version 24.02.10.
SUB16040196
CAM073016_FW_48