PacBio HiFi sequencing data for Paralichthys californicus
The HiFi SMRTbell library was constructed using the SMRTbell prep kit 3.0 (Pacific Biosciences, MenloPark, CA; Cat. #102-182-700) according to the manufacturer's instructions. HMW gDNA was sheared to atarget DNA size distribution between 15-18 kb using Diagenodes Megaruptor 3 system (Diagenode,Belgium; cat. B06010003). The sheared gDNA was concentrated using 1X of SMRTbell cleanup beadsprovided in the SMRTbell prep kit 3.0 for the repair and a-tailing incubation at 37C for 30 minutes and65C for 5 minutes, followed by ligation of overhang adapters at 20C for 30 minutes, clean-up using 1XSMRTbell cleanup beads, and nuclease treatment at 37C for 15 minutes. The SMRTbell library was sizeselected using 3.1X of 35% v/v diluted AMPure PB beads (Pacific Biosciences, Menlo Park, CA; Cat. #100-265-900) to progressively remove SMRTbell templates <5kb. The 15  18 kb average HiFi SMRTbelllibrary was sequenced at UC Davis DNA Technologies Core (Davis, CA) using one 8M SMRT cell (PacificBiosciences, Menlo Park, CA; Cat #101-389-001), Sequel II sequencing chemistry 2.0, and 30-hour movieseach on a PacBio Sequel IIe sequencer.
SUB14819288
CCGP_gDNA0116