WGBS of mus musculus: corneal epithelium, 14 months
WGBS libraries were prepared using xGen Methyl-Seq DNA Library Prep Kit (#10009860, Integrated DNA Technologies, Inc.) according to manufacturer's instructions. Briefly, 100ng of gDNA was spiked with 1% unmethylated Lambda gDNA and fragmented to 350bp (Covaris M220, Covaris, LLC, Woburn, MA, USA). Bisulfite conversion was accomplished using the EZ DNA Methylation-Gold Kit (D5005, Zymo Research, CA, USA). Adaptase was used to ligate adapters to the 3 end of the bisulfite-converted DNA, followed by primer extension, second strand synthesis, and ligation of adapter sequences at its 3 end. The libraries were amplified for a total of 6 rounds using the KAPA HiFi Uracil+ PCR enzyme. WGBS libraries were quantified using Qubit 4 Fluorometer, and sizes were evaluated using the 4200 TapeStation system.
SUB14818528
CE14m_3_wgbs_O3