GBS of Cichorium intybus: sample 'Puntarelle isolate 7'
double digestion of gDNA with PstI and MspI restriction enzymes, ligation to sequencing adapters with barcodes, pooling, enrichment by PCR, size selection with beads to 100-600bp fragments length, sequencing on ionTorrent platform Ion GeneStudio S5 Plus (protocol adapted from Abed et al., 2019)
SUB14887729
Punt2_7