Ec19
Sample preparation begins with the extraction of genomic DNA from the E. coli Rb12 strain. The extracted DNA is enzymatically fragmented (tagmented), followed by end repair and the addition of a single adenine (A) nucleotide to the 3' ends of the fragments (A-tailing) to facilitate subsequent adapter ligation. During library construction, dual-indexed sequencing adapters are ligated to the prepared fragment ends; these adapters are essential for binding the DNA fragments to the flow cell and initiating the sequencing process. The final prepared library is then loaded onto a nanopore flow cell for sequencing, where the resulting ionic current signals are decoded in real-time by basecalling algorithms to determine the nucleotide sequence
SUB15392101
Ec19_1