A single adult Diploria labyrinthiformis coral colony N32 from the waters of Bayahibe Dominican Republic
Coral tissue from ~0.2-0.5 mg samples were lysed in 0.5 mm glass beads (Millipore Sigma cat #19-622) immediately after collection at the field site. DNA was extracted using the Zymo Quick MiniPrep Plus Kit following the kits instructions for Samples in DNA/RNA Shield (https://echille.github.io/E.-Chille-Open-Lab-Notebook/Bhattacharya-Lab-Adult-Coral-DNA-Extraction-Protocol/). Samples were eluted in 100 uL of molecular water and sent for sequencing and library preparation at Azenta Life Sciences (South Plainfield, NJ). Library preparation and sequencing followed the Azenta protocol for Short Read Non-Human Whole Genome Sequencing), targeting a read length of 250 bp and ~30x depth of coverage for a 1000 Mbp genome.
SUB15391997
Diploria labyrinthiformis N32