16S rRNA amplicon from 10-20cm sediment sample DN001, Three Gorges Reservoir
Adapters were ligated to the flanking regions of the target sequence by PCR, and the resulting PCR products were recovered by gel extraction using a gel recovery kit. The purified products were eluted with Tris-HCl buffer, and the eluate was detected by 55% agarose gel electrophoresis for quality control. Finally, the qualified products were denatured with sodium hydroxide to obtain single-stranded DNA fragments. The main reagent used in this process was the TruSeq DNA Sample Prep Kit.
SUB15981460
DN001_LIB03