ONT of Macauba
High-molecular-weight (HMW) DNA was extracted using a protocol based on Jones et al.12 and the yielded HMW DNA was used for all ONT libraries. HMW DNA samples were purified with AMPure XP beads (Beckman Coulter Inc., CA, United States), and DNA fragments ranging from 10 to 25 Kbp were selectively depleted using the SRE kits from PacBio and ONT. Quality control was performed using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific, MA, USA), and the A260/280 and A260/230 ratios were assessed to determine DNA purity. Genomic DNA concentration was confirmed using a Qubit 3.0 fluorometer (Invitrogen, CA, USA) with the dsDNA BR Assay Kit (Thermo Fisher Scientific, MA, USA, Q32850). The integrity and size of high-molecular-weight (HMW) DNA fragments were evaluated using the 4200 TapeStation system (Agilent Technologies, Santa Clara, CA, USA). ONT long-read sequencing was performed using the SQK-LSK114 kit and three FLO-PRO114M flow cells on the P2-Solo platform. Corrected reads with HERRO.
SUB15833499
ONT