scRNA of Pool93_40, tcr profiling
Frozen aliquots of cells were thawed into RPMI with 10% FBS. Samples were washed with media and a dead cell depletion (Miltenyi Biotec 130-090-101) was performed prior to staining of all viable cells with cell hashtag antibodies (TotalSeq-C, Biolegend). After washing, samples were pooled and stained with cell surface antibodies (TotalSeq-C, Biolegend) according to manufacturer's protocol prior to single-cell RNA-sequencing (10X genomics, 5' kit with TCR and BCR). A total of 40,000 cells were loaded onto eight 10x channels. The scRNA-seq libraries were processed using Chromium Next GEM Single Cell 5' Kit v2, and the TCR/BCR libraries were done using Chromium single cell human TCR/BCR amplification kits, and the hashing libraries were processed using Chromium 5' feature barcode kit (10× Genomics).
NCI-phs004175
Pool93_40-tcr