HC4T2
Tissue was extracted for DNA using the E-Z 96 Tissue DNA Kit (Omega Bio-Tek, Norcross, GA). 10 ng of total DNA was used for input, and the libraries were amplified for 7-9 cycles of PCR using SG UDI Primers (Singular Genomics, San Diego, CA) rather than Revivity Primer Mix V2. Final libraries were eluted in 5 mM TRIS, and library quality and concentrations were determined using the High Sensitivity DNA Kit on Agilent Bioanalyzer 2100 and Qubit spectrofluorometer (ThermoFisher Scientific, Waltham, MA). Samples in each group were pooled equally based on total concentration (ng).  Samples were sequenced as paired-end 150 bp reads using the Singular Genomics G4 system.
SUB15905234
HC4T2