TIGIT PhotoRaPID selection
DNA libraries, including 6-12 degenerate NNK codons in a ratio 0.001 NNKn=6:0.032 NNKn=7:1 NNKn=8:32 NNKn=9:33 NNKn=10:33 NNKn=11:11 NNKn=12 (5-TAATACGACTCACTATAGGGTTAACTTTAAGAAGGAGATATACATATG(NNK)nTGCGGCAGCGGCAGCGGCAGCTAGGACGGGGGGCGGAAA) were subjected to 9 rounds of RaPID selection using hPADI4 as protein target and different buffer conditions. Double indexed libraries (Nextera XT indices) were prepared with the DNA recovered after each round and sequenced on Illumina NovaSeq X Plus with a 10B Flowcell size and 100 nt paired-end reads (200 cycles total)
SUB16260865
TIGIT_Round_4