ONT WGS of male HTLV-1+ hu-NSG mouse: adult CD8+ T-cells sorted from blood
DNA was extracted from sorted T cell populations using the Monarch HMW gDNA extraction kit, followed by shearing to ~20 kb using a Covaris G-tube. DNA libraries were prepared using the genomic DNA by ligation kit (SQK-LSK114) and were sequenced on an Oxford Nanopore Technologies (ONT) PromethION platform (r10.4.1 chemistry). Modified bases were called using Dorado v0.3.4 (Oxford Nanopore Technologies).
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