RNA-Seq of Homo sapien:Adult male retina
Watchmaker RNA Kit with Polaris Depletion (Watchmaker Biotech) as per the manufacturers instructions. The Polaris Depletion reactions were performed at full scale and the library preparation reactions were performed at half scale. RNA fragmentation, first-strand cDNA synthesis, second-strand synthesis, A-tailing, and adapter ligation were performed following the kits instructions. The libraries were amplified, and a post-ligation cleanup was applied using SPRI beads (Beckman Coulter).  The final libraries were sequenced on the Illumina NovaSeq6000 S1 300 Cycle in 2x150bp fashion, producing between 46.8 and 80.5 million reads per sample.
SUB15683541
2024-1259OD-12Seq_S5_L001_R1