RNA-seq of crab
mRNA was purified from total RNA using the magnetic bead method, and the intact mRNA was fragmented using a Bead Wash Buffer and a metal bath. First-strand cDNA was synthesised using the above mRNAs as templates. The second strand cDNA was then synthesised using Resuspension Buffer, EtOH, and Buffer. cDNA was immediately followed by end repair. Finally, the constructed cDNA library was sequenced by Illumina, and the sequencing was commissioned to Beijing Novogene Technology Co. (China)
SUB14979317
FRAS230324889-5r