16S rRNA sequencing of apple ovary, pollen, seed and shoot microbiome
DNA extraction was performed using the FastDNA SPIN Kit for Soil (MP Biomedicals). The 16S rRNA gene was amplified using primers 515f and 806r (Walters et al., 2015). PCR reactions were performed in a total volume of 30 L and were purified using the Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA). Samples were sequenced using 250 bp paired-end Illumina MiSeq sequencer at Institute of Environmental Biotechnology, Graz University of Technology, Graz, Styria, Austria
SUB15551701
t1_T7_S4