Cornus kousa K2 HiFi Run 5
K2 young leaves were collected from the Rutgers Gardens and flash frozen in liquid nitrogen. Bionano Prep Plant Tissue DNA Isolation kit (Bionano Genomics, San Diego, CA, U.S.) was used to isolate nuclei from the bud tissue. High molecular weight (HMW) DNA was extracted from the nuclei with Circulomics Nanobind Plant Nuclei Big DNA Kit (Pacific Biosciences, Menlo Park, CA, U.S.). HMW DNA was sheared to ~15kb fragments with the Covaris g-TUBE (Woburn, MA, U.S.). The sheared DNA was used to prepare a PacBio SMRTbell library and size selected using BluePippin (Sage Science, Beverly, MA, U.S.). The library was sequenced on three Sequel IIe (Pacific Biosciences, Menlo Park, CA, U.S.) cells.
SUB15738021
K2_Run5_m64310e