Oxford nanopore reads of full-length cDNA of ANK3 extracted from frontal cortex and amplified from TSS1
ANK3-specific amplification was performed with 28x13 minute long extension cycles using a time increase per subsequent cycle (+20 seconds) using primers designed for either transcription start site 1 (TSS1) or TSS2 together with a common reverse primer. Mouse primers: Ank3_1F(TSS1)=GTGGAGAAGCAAGCCCTGAAA, Ank3_4F(TSS2)=AGGCTCAGCAAGGTGTAGGA, Ank3_R=TCAGTGCCGGGAATGAATGT
SUB16125599
mouse.fc.tss1.filt