16S-Seq of Pygoscelis papua: fecal microbiome at 30 day
Bacterial genomic DNA was extracted from each sample using a QIAamp Power Fecal DNA Kit (Qiagen, California, USA), following the manufacturer's instructions. Following quality control via 1.5% agarose gel electrophoresis, the extracted DNA was used to amplify the V3V4 regions of the bacterial 16S rRNA gene with the 341F (CCTACGGGNGGCWGCAG) and 806R (GGACTACHVGGGTATCTAAT) primers (Lee et al., 2023). The detailed processes for the polymerase chain reaction and sequencing library construction have been reported in a previous study by Tian et al. (2024). The constructed libraries were sequenced on an Illumina NovaSeq 6000 platform using a 250 bp paired-end strategy at Personalbio Co., Ltd., Shanghai, China.
SUB15759525
Pup_30d_3