High-throughput sequencing of Corydalis wilsonii leaves
Fragment the DNA, perform size selection using magnetic beads to isolate fragments of 200-400 bp, followed by end repair and A-tailing to add an 'A' overhang at the 3' end. Ligate adapters to the processed fragments. Conduct PCR amplification, purify the amplified products, denature them into single-stranded DNA, and perform circularization to generate the final sequencing-ready library.
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