Exome sequencing of PDX establsihed form breast cancer samples
Briefly, the tumors were mechanically disaggregated with a scalpel and ground in a mortar in the presence of dry ice. Then, the crushed tumor was incubated in digestion buffer for 3 hours at 55C and centrifuged. DNA was extracted with the phenol-chloroform-isoamyl method. Library were prepared using Agilent library kit SureSelect V6-Post following manufacturers instructions
SUB14695309
BC-AR631