Whole mitochondrial genome sequencing of Sus scrofa: Spleen from Pig 3.3
Genomic DNA was extracted from total DNA was extracted from Sus scrofa tissue using the DNeasy Blood & Tissue Kit (QIAGEN). Four overlapping mtDNA amplicons, size 7262 bp, 3141 bp, 3235 bp, and 3657 bp, were amplified separately using primer Sus1 (Fragment1F=GTTTAACGGCCGCGGTATTC, Fragment1R= TGATTACGTGCAACCCGTGA), Sus2 (Fragment2F=TCACGGGTTGCACGTAATCA, Fragment2R= AATGTCTCCGATGCGGTTGT), Sus3 (Fragment3F= CAGACCGTAGAACTCTCTCTTAGC, Fragment3R= GAACGGGTGTTCTACGGGTT), and Sus4 (Fragment4F= CGTTCAATTCCTAATAAACTAGGTGG, 4= CCTTATTTGGAGAACAAGTGATTATGC), respectively. The amplicons were pooled, and the library was prepared using Prep-M library preparation with 12 amplification cycles. Four overlapping mtDNA amplicons, size 7262 bp, 3141 bp, 3235 bp, and 3657 bp, were amplified separately using primer Sus1 (Fragment1F=GTTTAACGGCCGCGGTATTC, Fragment1R= TGATTACGTGCAACCCGTGA), Sus2 (Fragment2F=TCACGGGTTGCACGTAATCA, Fragment2R= AATGTCTCCGATGCGGTTGT), Sus3 (Fragment3F= CAGACCGTAGAACTCTCTCTTAGC, Fragment3R= GAACGGGTGTTCTACGGGTT), and Sus4 (Fragment4F= CGTTCAATTCCTAATAAACTAGGTGG, 4= CCTTATTTGGAGAACAAGTGATTATGC), respectively. The amplicons were pooled, and the library was prepared using Prep-M library preparation with 12 amplification cycles. Sequencing was performed on Illumina NovaSeq X 10B Lane, 300 cycles using 150bp paired end sequencingn cycles. Sequencing was performed on Illumina NovaSeq X using 150bp paired end sequencing.
SUB15031778
3P_S