RNA-Seq data of JP223922_Cont_WW_31d_rep1 sample
The youngest fully expanded leaves of each rice accession grown in iPUPIL were collected for RNA extraction. Total RNA was extracted from shoot sample by guanidinium thiocyanate and isopropyl alcohol-based RNA extraction method. Equal amount of RNA extracted from three independent plants in the same pot was used for RNA-seq libraries. RNA-seq libraries were prepared using the NEBNext Ultra II Directional mRNA-seq kit (New England Biolabs) according to the manufacturers protocols. These libraries were sequenced using illumina NovaSeq 6000. The sequencing was performed in S4 flow cells with paired-end 150-bp and unique dual index reads.
SUB15563741
W130