Oxford Nanopore ultra-long DNA sequencing for genome assembly
Genomic DNA (gDNA)was extracted from themuscle tissue of Hemibarbus labeo according to the instruction of Monarch HMW DNA Extraction Kit for TissueNEBT3060L. DNA quality was analyzed using NanoDrop (Thermo Fischer Scientific, ND2000), QubitInvitrogen, Q33238, and CHEF Mapper XA Chiller System (Biorad, 1703672). After examination of the quality of isolated DNA, the library was constructed using the SQK-LSK109 Kit. The library was sequenced on Nanopore PromethION sequencer.
SUB16040431
Muscle_DNA_ONT_UltraLong_Lib3