skin microbiome of Heteronotia binoei at Ross River, NT, Australia
Gecko was hand-captured in the wild at night and sampled the following day, in the meantime kept in a clean, dry fabric bag. Gecko was rinsed with milli-q water before entire body swabbed with Copan flocked swab avoiding the face and cloaca. Samples was stored in 300uL Zymo DNA/RNA Shield, chilled in the field, and frozen at -20C until DNA extraction with Norgen's Microbiome kit. Amplicons of the bacterial V4 region of 16S rRNA gene were prepped and sequenced on MiSeq at Ramaciotti Centre for Genomics.
SUB14674901
HB429