deep amplicon sequence of canine faeces: Strongyloides stercoralis
Total DNA was isolated from 250 mg of fresh faeces and stored at -20C prior to PCR. The nuclear 18S rDNA (HVR-I and HVR-IV) and mitochondrial cox1 PCR assays were used to type Strongyloides spp. Sample was amplified with SensiFAST SYBR No-ROX Kit (Bioline Australia). All primers were adapted for Illumina sequencing. The assay was run on CFX Opus 96 Real-Time PCR System (Biorad, Australia). Amplicons were submitted for amplicon deep sequencing using Illumina NGS on MiSeq i100 (300PE).
SUB15773587
JS7413