RNA-seq A21-E4-10 of AS pt 206 10% PBMCs
Whole blood was collected into EDTA tubes from patients newly diagnosed with prostate cancer. PBMCs were purified from whole blood using the MACSprep PMBC Isolation kit. Cell viability was assessed using a Luna FX7. Flow through was collected to generate mixtures. Buffy coats were collected from whole blood drawn into EDTA tubes. RNA was extracted from frozen buffy coats using the Macharay-Nagel NucleoSpin RNA Blood kit and from purified PBMCs using the Qiagen RNeasy Mini Kit. Libraries were prepared with the NEBNext Globin and rRNA depletion library construction kit and the Illumina TruSeq stranded total RNA with ribo-zero globin kit.
NCI-phs001813
A21-E4-10