skin microbiome of Gehyra koira; NT, Australia
Gecko was hand-captured in the wild at night and sampled that night or the following morning, in the meantime kept in a clean, dry fabric bag. Gecko was rinsed with milli-q water before entire body swabbed with Copan flocked swab avoiding the face and cloaca. Samples was stored in 300uL Zymo DNA/RNA Shield, chilled in the field, and frozen at -20C until DNA extraction with Qiagen DNeasy Blood & Tissue Kit.  Amplicons of the bacterial V4 region of 16S rRNA gene (EMP 515/806 primers) were prepped and sequenced on MiSeq at Ramaciotti Centre for Genomics.
SUB14680583
GK369