HiC sequencing: female Trachinotus anak
For Hi-C sequencing, fresh liver tissue was collected for library preparation as previously described9, with minor modifications. Briefly, chromatin was cross-linked with 1% formaldehyde, digested with DpnII, end-repaired with biotin-14-dCTP, and proximity-ligated using T4 DNA ligase. After reversing cross-links and purifying DNA, the DNA was sheared to fragments of 300700 bp and enriched using streptavidin magnetic beads. The Hi-C library was constructed using with MGIEasy Universal DNA Library Preparation Kit v.1.0 (MGI, China) and sequenced on DNBSEQ-T7 platform.
SUB15411037
Tana-HiC