Shotgun metagenomics of pooled human saliva grown in streptococcal CDM with the addition of PBS, tryglysin A, or reverse SHP peptide from S. mutans.
Shotgun metagenomic sequencing libraries were prepared with Illumina DNA Prep kit (Illumina, 20060059) per manufacturer's recommendations. Library selection was performed using this kit, which uses bead linked transposome chemistry (BLT) to integrate DNA extraction, fragmentation, library preparation and library normalization. This results in a size-based selection and labeling with required indexes and/or adapters.
SUB14889593
BR5-revSHP-R1