SSU rRNA gene_Day812
A universal primer set for SSU rRNA gene sequences, 926 F (5-AAACTYAAAKGAATTGACGG-3) and 1392 R (5-ACGGGCGGTGTGTRC-3) was employed, targeting the V6 to V8 regions of bacteria (16S rRNA), archaea (16S rRNA) and eukaryotes (18S rRNA). Sequencing was performed on an Illumina MiSeq platform (Illumina, USA) at the Australian Centre for Ecogenomics, The University of Queensland, Brisbane, Australia. Amplicon clustering of the sequencing data and taxonomy assignment were carried out using QIIME2 and BLAST, respectively, according to the ACE amplicon pipeline (https://acepipe.readthedocs.io/en/latest/).
SUB15653487
Day812