DNA-seq of Klebsiella pneumoniae
The genomic DNA was extracted by using the Cetyltrimethyl Ammonium Bromide (CTAB) method with minor modification, and then the DNA concentration, quality and integrity were determined by using a Qubit Flurometer (Invitrogen, USA) and a NanoDrop Spectrophotometer (Thermo Scientific, USA). Sequencing libraries were generated using the TruSeq DNA Sample Preparation Kit (Illumina, USA) and the Template Prep Kit (Pacific Biosciences, USA).The genome sequencing was then performed by Personal Biotechnology Company (Shanghai, China) by using the Nanopore PromrthION48 platform/(Pacific Biosciences platform) and the Illumina Novaseq platform
SUB16331909
KPN0176_library