Multiome seq of prostate tissue of AS pt 79, ATAC (set 2 of 6)
Fresh tissue biopsies were acquired from patients newly diagnosed with prostate cancer. Tissues were minced and flash frozen. Nuclei were recovered using the 10X nuclei isolation kit. Nuclei samples were transposed according to 10x Genomics Chromium Next GEM Single Cell Multiome ATAC + Gene Expression User Guide. The transposed nuclei were then loaded into the lanes, with one capture lane per sample targeting recovery 6,000 nuclei per lane. Partitioning was successfully completed with uniform emulsion consistency. Reverse transcription and barcoding followed immediately after partitioning. All subsequent steps of library preparation and quality control were performed as described in the 10X user guide.
NCI-phs001813
SCAF4477_mATAC_S18_L002