RNAseq sequencing of Talaromyces verruculosus TS63-9 control sample 1
Talaromyces verruculosus TS63-9 was inoculated on basic enzyme producing medium (yeast extract 1.0 g/L, (NH(NH4)2SO4 6.0 g/L, KH2PO4 3.0 g/L), pectin 5.0 g/L was added to the treated sample, while no pectin was added to the control.  After 24 hours, the liquid medium was centrifuged at 8000rpm with 5min to collect in a 50ml centrifuge tube.  There are three biological replicates in both control( named C1, C2, C3)  and pectin-treated samples(named T1, T2, T3). Total RNA from Talaromyces verruculosus TS63-9 was prepared with Qiagen RNeasy plant mini Kit(Qiagen, Germany). The quality of RNA was checked by agarose electrophoresis. Total RNA obtained was subjected to quality control by agarose gel electrophoresis.  Two RNA libraries were constructed: a paired-end library with an insert size of 500 bp. The 500 bp library and the 5kb library were sequenced using an Illumina HiSeq2500 by PE150 strategy. The DNA library construction, sequencing and assembly were all performed at the Sangon Biotech (Shanghai) Co., Ltd. Quality control of sequence reads was performed using in-house programs including adapter trimming and removal of low quality reads. The filtered reads were mapped to 11326 genes of T. verruculosus  TS63-9 by using CLC genomics workbench. The relative expression value of TPM was calculated.
SUB15031660
C1