100 males from line male-biased A at generation 1.
DNA was extracted using the MagJet Genomic Kit , following the manufacturers protocols. Genomic library preparation was conducted using the Nextera NebNext Ultra FS II kit. Sequencing was performed at the SNP&SEQ Sequencing Facility in Uppsala on a NovaSeq 6000 system, using S4 flow cell to produce 2 x 150 bp paired-end reads.
SUB15933246
SR20