WGS of non-UDG treated aDNA sample of Homo sapiens: Tooth
To obtain and authenticate aDNA, library preparation for whole genome sequencing was carried out using a modified protocol for aDNA, incorporating steps to mitigate potential DNA damage and fragmentation inherent to ancient samples. Briefly, end-repair, adapter ligation, and DNA indexing were performed using a combination of enzymatic and chemical processes. Unique barcoded adapters were used to allow for multiplexing of samples in a single sequencing run. Library amplification was conducted with a limited number of cycles to prevent over-amplification of damaged DNA molecules. Whole genome sequencing was carried out on an Illumina sequencing platform using paired-end sequencing reads.
SUB15508200
N6_S2