DNA sequence of E6 SD rats : colon contents
After the total DNA of the sample was extracted, specific primers with Barcode were synthesized according to the full-length primer sequence, amplified by PCR, and the products were purified, quantified and homogenized to form sequencing library (SMRT Bell). The completed library was inspected first. Qualified libraries were sequenced using PacBio Sequel. The data from PacBio Sequel is in bam format, and CCS files are exported through smrtlink analysis software. The data of different samples are identified according to Barcode sequence and converted into fastq format data.
SUB14816351
E6