WGS of xenograft: plasma cfDNA
EDTA plasma collected from 9-11 mice with HepG2 and HuH7-derived xenograft tumor was pooled, respectively. cfDNA was exacted from 4.2 mL pooled plasma using the QIAamp MinElute ccfDNA Midi Kit (Qiagen, Hilden, Germany) with the QIAcube platform, according to the manufacturer's instructions. After extraction, the DNA fragment size and percentage of cfDNA were determined with electrophoresis using the Cell-free DNA ScreenTape Analysis on TapeStation 4200 fragment analyzer (Agilent Technologies Inc.). cfDNA concentration was determined by the Qubit HS-dsDNA Assay Kit and Qubit Flex Fluorometer (Thermo Fisher Scientific, Massachusetts , USA). Existence of cfDNA in each sample was QCed by confirming the single peak around 150bp with electropherogram (Fig. S5). The library preparation for whole genome sequence were conducted at Azenta laboratory with VAHTS Universal Pro DNA Library Prep Kit for Illumina ND608-02 (Vazyme biotech, Nanjing, PRC). NGS assay were conducted Pair-end sequencing of 2 x 150 bp was conducted using an illumina Novaseq6000 according to the manufacturers instructions at Azenta laboratory.
SUB14791544
WGS_2