DAP-Seq-Input
Genomic DNA from R. roxburghii was extracted, and enriched DNA was sheared into short fragments by ultrasonication. The fragmented DNA was then end-repaired, 3 A-tailed, and ligated to Illumina sequencing adapters. DNA fragments of appropriate size, typically 100300 bp including adapter sequences, were selected and PCR-amplified. Qualified libraries were subsequently sequenced on an Illumina No-vaSeq 6000 platform by Gene Denovo Biotechnology Co. (Guangzhou, China). After raw-read filtering, clean reads from each sample were aligned to the reference genome with accession number CNP0004212 (https://db.cngb.org/cnsa/) using Bowtie2 v2.2.5
SUB16227336
DAP-Seq-Input