cDNA sequence capture of Picea glauca: foliage of sample 37-R5
cDNA was synthesised with Maxima First Strand cDNA Synthesis Kit and NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module. cDNA was purified, end-repaired, dA-tailed, and then ligated with adaptors for Illumina using the NEBNext Ultra II Library Prep Kit for Illumina. The sub-libraries were then amplified and barcoded. Hybridisation capture was performed with myBaits Custom 120K Kit using the probe set Picea_hung_p1.0. The target-enriched sub-library was amplified. All sub-libraries were pooled and normalised. Nanopore libraries were constructed using the Ligation Sequencing Kit Chemistry 14 and sequenced on a R10.3 flow cells on a GridION system. Nanopore reads were basecalled and demultiplexed from raw electrical signals using Guppy v6.0.0, trimmed for Nanopore and/or Illumina adaptors and split for chimeras using Porechop 0.2.4.
SUB14788804
37-R5