NGS of human samples to confirm cytosine base editorinduced Q217X mutation in HEK293-GABA cells.
RNA was converted to cDNA, target was amplified using specific primers. Amplicons were pooled and purified using AMPure XP bead purification for size selection to enrich for amplicons of the appropriate size prior to Illumina NovaSeq sequencing
SUB16193871
GABA CBE Q217X_GABA GJB2 KO Tx2_B