HBSAb-CDRL1 amplication
Genomic DNA was extracted from 510^6 cells. A two-step PCR was used for library preparation: first PCR with Q5 High-Fidelity DNA Polymerase (98C for 30 sec; 16 cycles of 98C for 10 sec, 70C for 20 sec, 72C for 30 sec; final extension 72C for 1 min); second PCR (98C for 30 sec; 2 cycles of 98C for 10 sec, 40C for 20 sec, 72C for 1 min; 6 cycles of 98C for 10 sec, 65C for 20 sec, 72C for 1 min; final extension 72C for 5 min). Purified libraries were sequenced on the Illumina MiSeq platform (2250 bp).
SUB15809656
1-1