ONT sequencing of Drosophila melanogaster JW18 cells colonized with wMel Wolbachia
High-molecular-weight DNA was extracted from JW18-wMel whole cell lysate using the Zymo Quick-DNA HMW MagBead Kit, following the manufacturers instructions for cells and solids. Nanopore libraries were prepared using the Rapid Barcoding Kit 24 V14, sequenced on a MinION Mk1C with an R9.4 flow cell (#FAX48436), and base-called with Dorado v0.5.3.
SUB15233251
ONT_JW18_wMel