Bacterial amplicons corresponding to the area not affected by yellow stain
For bacteria, the V4 region of the 16S ribosomal RNA gene was targeted using specific primers. DNA amplicon libraries were generated by PCR, followed by the addition of Illumina sequencing adapters and dual-index barcodes. The libraries were then normalised, pooled and loaded onto a MiSeq Reagent Cartridge spiked with PhiX control to enhance base calling during sequencing.
SUB14653490
CSNA_16S