Whole Genome Sequencing of P. aeruginosa: Hospital Drinking Water
Genomic DNA was extracted from pure cultures of Pseudomonas aeruginosa using the Qiagen DNeasy Blood & Tissue Kit. Library preparation was performed using the Illumina DNA Prep kit (formerly Nextera DNA Flex) following the manufacturer's instructions. Libraries were quantified using a Qubit Fluorometer and quality-checked on an Agilent 2100 Bioanalyzer. Sequencing was conducted on the Illumina NextSeq 2000 platform at the icddr,b Genome Centre using a 300-cycle (2 x 150 bp) P2 Flow Cell to achieve a target coverage of approximately 100x per genome.
SUB16179764
KYAU_MB_PA_S96