16s amplicon seq of mus muculus:adult female feces
Fecal pellets from 6 conventional C57Bl/6 mice were pooled  and homogenized in PBS. 50 ul fecal slurry was inoculated into mGAM media containing either saline, DMSO, low conc loperamide in DMSO, high conc loperamide in DMSO, low conc imolope in saline, or high conc imolope in saline. Batch fermentation ran for 72 hours and 500 ul samples were collected every 24hour. Samples were centrifuged and DNA was extracted from the pellets with Qiagen Powersoil DNeasy kit. DNA concentrations measured using Qubit 2.0 dsDNA HS kit (Qiagen) and adjusted to 5 ng/ before PCR. V3-region 16S rRNA genes was sequenced using the Ion Torrent GSS5 platform in DTU DMAC core as previously described (Christensen et al. 2014).
SUB15234422
S55_Grp1_A_T24