sequences of bacteria
This study aims to analyze the composition of gut microbiota in sleep-deprived mice in relation to coffee consumption and its connection to health. Fecal samples were collected from C57BL/6J mice, and genomic DNA was extracted using the Omega Mag-bind Soil DNA Kit. Primers were designed for the V3-V4 region of the 16S rRNA gene, followed by PCR amplification. Sequencing was performed using the Illumina NovaSeq platform with the NovaSeq 6000 SP Reagent Kit. The raw data underwent quality control and OTU clustering analysis using QIIME2 DADA2, followed by differential analysis.
SUB14858965
NC1