RNA-seq of Phaffia rhodozyma: CBS 6938 sample 3
Total RNA was extracted from 48 h cell cultures with TRIzol reagent, quantified using NanoDrop 2000 spectrophotometer (Thermo Scientific, USA), and assessed for integrity on an Agilent 2100 Bioanalyzer (RIN  8.0). Next, mRNA enrichment was performed using the total RNA and Oligo(dT) magnetic beads. A cDNA library was constructed using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, USA). The library was sequencing via Illumina HiSeq X Ten (Illumina; 150 bp paired-end), generating about 50 million raw reads per sample.
SUB15389568
PTR1T20250616-WT3