16S-seq of sediment: mangroves
The V3-4 regions of 16S rRNA gene of bacterias were amplified with primers 338F/806R.PCR was carried out as follows: 3 minutes of pre-denaturation at 95, 30 seconds of denaturation at 95, 30 seconds of annealing at 55, and 45 seconds of extension at 72, for a total of 35 cycles.
SUB16070562
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