RNA sequencing of corn husk samples during fermentation
Genomic DNA was conducted with the TIANNAMP Soil DNA Kit following the manufacturer protocols. Microbial communities were explored by high-throughput sequencing on Illumina MiSeq PE250 platform in Shanghai Majorbio Bio-pharm Technology Co.,Ltd. Amplification of the 16S rRNA gene V3-V4 region utilized universal 516F/806R primer for both the bacteria and archaea communities. The obtained clean sequences were clustered into operational taxonomic units (OTUs) with a 118% sequence similarity threshold
SUB15520539
FJ_0420_8_X_1