16S rRNA amplicon sequencing of ferruginous duricrust soils: AM12
The DNA was extracted from 250 mg of each sample using the DNeasy PowerSoil Pro Kit (Qiagen, Germany) following the manufacturers instructions. The amount of DNA was quantified using a NanoDrop Spectrophotometer (Thermo Scientific). The PCR amplification and sequencing were performed in the Zymo Research Corporation (Botucatu  SP, Brazil). The pair of primers 341F (CCTACGGGRSGCAGCAG) and 806R (GGACTACHVGGGTWTCTAAT) was used to amplify the V3-V4 region of the 16S rRNA gene following the company's own protocol. The amplicons were sequenced using the Illumina MiSeq platform.
SUB15528935
AM12_DNA