16S amplicon sequencing of sample T19GI_F3
The 16S full-length rRNA amplicons were purified followed by indexing using Nanopore-specific barcode. Then, the indexed PCR products were pooled and cleaned up with 0.5 x vol Ampure Bead XP.  Following quantification using Qubit, a total of 200 fmol input DNA were used for the LSK114 Nanopore library prepation kit following the manufacturere's instructions. Sequencing was performed on a Flongle R10.4.1 for 12 hours and basecalling using Dorado v0.7.0 super accuracy mode v5.0
SUB15004614
T19GI_F3