Bulk RNA-Seq of mus musculus: RT_2 (BV2 cell line)
Total RNA was extracted from BV2 cells using TRIzol, and mRNA was enriched with poly-T magnetic beads. Stranded cDNA libraries were prepared using random hexamer priming, end repair, A-tailing, adapter ligation, and PCR amplification. Libraries were sequenced on an Illumina platform, and raw reads were processed with fastp to remove adapters and low-quality sequences.
SUB16129815
RT_2