Leishmania donovani, axenic amastigote, 37C, pH 5.5, sample 2
Nuclei from axenically grown mammalian stages (amastigotes) were used in a Precision Run-On/sequencing assay (PRO-seq) to allow abortive trasncription elongation by pre-existing RNA polymerase complexes. Affinity-labeled nascent RNA was purified, processed into Illumina sequencing libraries and analysed by NextSeq500 high throughput sequencing. Reads were then trimmed and aligned to the L. donovani genome to map positions and read densities genome-wide. By comparing RNA polymerase positions and densities, wie mapped transcription initiation and -termination regions and compared densities between developmental stages.
SUB15078439
Ama2