ChIP-seq of At: seedlings
Fifteen-day-old plants were homogenized in liquid nitrogen and fixed in 1% formaldehyde for 20 minutes. After quenching with glycine and cell lysis, chromatin was sheared using a Bioruptor sonicator. ChIP-seq libraries were prepared using a NEBNext Ultra II DNA Library Prep Kit for Illumina. DNA fragments were end-repaired and ligated to sequencing adapters. Library size distribution was assessed using an Agilent 5400 system and quantification was performed via qPCR.
SUB15419426
C5_Input