ONT cDNA of term villous placenta
Placental RNA was extracted from villous tissue using phenol-chloroform and purified with a Qiagen RNeasy Mini Kit (GUSTO cohort) or Qiagen QIASymphony DSP DNA Midi Kit (unpublished cohort). cDNA libraries were prepared using the Oxford Nanopore PCR-cDNA kit (SQK-PCB111.24) with oligo(dT) priming and sequenced on a PromethION (R9.4.1) platform in 24-plex runs, generating median read lengths of ~1 kb. FAST5 files of raw ONT signals were base called using Guppy v6.5.7 on a GPU with barcode adapter trimming enabled.
SUB15428208
SampleID_019-30104