IgG control for 140_GW17_GO EN
Radial glia (RG),  Intermediate progenitor cells (IPC) and Excitatory neurons (EN) were purified from human cortical brain tissue at gestational weeks (GW) 17 using flourescence activated nuclei sorting (FANS).  Sorted nuclei were incubated with Fab fragments to block antibodies used in FANS and ~25,000 nuclei were  subjected to GO-CaRT/CUT&RUN using IgG antibodies as controls. Sequencing libraries were constructed using KAPA Hyperprep kit following manufacturer's instructions.  Libraries were sequenced using 150-bp paired-end sequencing on Illumina NovaSeq X Plus.
NIMH-phs000989
GW17_140_EN_IgG