Multi-locus metabarcoding of T. cruzi: T. sanguisuga abdomen
Six genetic markers were amplified: the mitochondrial gene Cytochrome b (CytB) and five nuclear loci (Pumilio/PUF RNA-binding protein (RB), Pyruvate dehydrogenase (PDH)), and three newly developed markers (17c, 18c, 17d). Purified PCR products were pooled in equimolar concentrations and sequenced on the Illumina MiSeq platform.
SUB15418907
FL4I3C