PCR-cDNA seq of Mustela putorius furo: in vivo IAV-infected 6H 4
One adult outbred ferret (600-1500 gram) was inoculated intransally with seasonal influenza-A (IAV) A/Perth/265/2009 (Perth265, H1N1pdm09) via 500 uL of PBS containing 107 PFU of Perth09. At 6 hpi, the ferret's nasal tissue was collected. The RNA was extracted using the RNeasy Mini kit, treated with DNase (Turbo Dna-Free Kit). The sequencing was carried out using the PCR-cDNA Barcoding Kit (SQK-PCB111.24, Oxford Nanopore Technologies), via the GridION for 72 hours.
SUB15371013
Vivo_IAV_6H_4