Lactiplantibacillus plantarum isolated from artisanal cheese
Genomic DNA libraries are prepared using the Nextera XT Library Prep Kit (Illumina, San Diego, USA) following the manufacturers protocol with the following modifications: input DNA is increased 2-fold, and PCR elongation time is increased to 45 seconds. DNA quantification and library preparation are carried out on a Hamilton MicrolabSTAR automated liquid handling system (Hamilton Bonaduz AG, Switzerland). Libraries are sequenced on an lllumina NovaSe 6000 (Illumina, San Diego, USA) using a 250 bp paired end protocol. For projects predating April, 2020, libraries were sequenced on an Illumina HiSeq 2500 using a 250 bp paired end protocol (Illumina, San Diego, USA). Reads are adapter trimmed using Trimmomatic version 0.30  with a sliding window quality cutoff of Q15. De novo assembly is performed on samples using SPAdes version3.7, and contigs are annotated using Prokka 1.11
SUB15735370
294724