RNA-Seq of Pseudomonas aeruginosa
This study aimed to investigate the transcriptional profiles of Pseudomonas aeruginosa.  Bacterial cells were harvested at the exponential growth phase (OD600=1.0)] for total RNA extraction. RNA quality was verified via Nanodrop. The resulting cDNA fragments were purified using the QiaQuick PCR Extraction Kit (Qiagen, Germany), followed by end repair, poly(A) tailing, and ligation of sequencing adapters. The adapter-ligated products were size-selected by agarose gel electrophoresis, amplified by PCR, and sequenced on the DNBSEQ platform (MGI Tech, China).
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tsrF_2