sequences of SIRT2  inhibitor treatment
DSP method was performed to analyze the spatial transcriptome from six mouse samples treated with Vehicle (n = 3) or AGK2 (n = 3), which supported from YuceBio. Tissues were stained with morphology markers DNA-FITC (Nuclei), CD45-Texas Red (tumor-infiltrating leukocytes), CD8-Cy5 (CD8+ T cells) to identify ROIs. Different compartments within the ROIs, determined by fluorescent staining with specific antibodies, were called areas of interest (AOIs). Oligos from each AOI were cut by ultraviolet irradiation. 144 AOIs were collected for next generation sequencing. Data normalization was performed using Quantile 3 normalization. Differential expression analysis was calculated by edgeR (P < 0.05 and |log2 fold change| > 0.5).
SUB15156350
Vehicle-48-DSP