16S rRNA amplicon sequencing of Mus musculus: vehicle-treated cecal content
Cecal contents were subjected to 16S rRNA gene amplicon sequencing targeting the V3-V4 region using 341F/805R primers on an Illumina MiSeq platform (paired-end, 2x250 bp). Paired-end reads were merged using VSEARCH, and primer sequences were trimmed using an in-house script (ChunLab/EzBioCloud pipeline). Low-quality reads were filtered based on sequence length, average Q-score, and 16S gene prediction by Hidden Markov Model-based search; singleton sequences were excluded. Taxonomic assignment was performed against the EzBioCloud 16S database using VSEARCH with a 97% similarity cutoff for species-level identification. Chimeric sequences were identified using UCHIME.
SUB16331629
Vehicle6