RNA-Seq of Cannabis sativa trichome: Pentyl Cannabinoid Type Biorep 1
RNA isolated using NucleoSpin Plant and Fungi RNA Isolation Kit. RNA quality and integrity were assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies) and RNA with an RNA integrity number (RIN)  7 was used for library preparation. RNA-Seq libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina, New England Biolabs according to the manufacturers instructions. Briefly, mRNA was isolated using oligo(dT) magnetic beads, fragmented, and reverse-transcribed into cDNA. Double-stranded cDNA was end-repaired, A-tailed, and ligated to sequencing adapters, followed by PCR amplification. The libraries were quantified using Qubit dsDNA HS Assay Kit, Thermo Fisher Scientific and their size distributions were assessed with the Agilent Bioanalyzer. Sequencing was performed on an Illumina HiSeq 4000 platform in 100 bp paired-end mode on a single lane, achieving an average read depth of approximately 20 million reads per sample.
SUB15417909
C5_S116