RNA-Seq Cx. annulirostris infected with JEV
Total RNA from JEV-infected and uninfected mosquitoes was extracted using Qiazol (Qiagen) according to the manufacturers instructions. The obtained RNA pellets were resuspended in 30 L of UltraPure DNase/RNase-free water (Invitrogen) and treated with DNase I enzyme (TURBO DNase, Invitrogen) to remove genomic DNA from RNA samples as per the manufacturers instructions. rRNA was depleted by Genewiz.
SUB14989793
JEV_infected_12D_R3