Transposon library sequencing of ECL22/pSAM-EC-Apr
Genomic DNA was randomly fragmented into 200-500 bp fragments, subjected to end repair and adenylation, then ligated with Illumina universal adapters. The target fragments were enriched by two rounds of amplification with adapter universal primer and transposon-specific primer to obtain the final sequencing library.
SUB15977206
ECL22_Tn