Pacbio sequencing of bacteria from one colony from a urine of a patient with a urinary tract infection
Microcolonies were selected from urine of a patient with a UTI infection on LB plate are kept at -80C. Bacteria were regrown in LB at 37C with shaking for 5h. DNA was extracted from 1 mL of bacteria using the DNeasy Blood & Tissue Kit (Qiagen).  Pacbio library preparation and sequencing was carried-out by BGI on one of the technical samples (UTI_30). SMRTbell prep kit 3.0 was used