Jurkat cell lines were transduced with pLJM1-EGFP or pLJM1-HTLV1c:H2BC12-HA, and 24 hours post transduction were selected with 2 g/mL puromycin for 5 days. 300,000 cells were lysed in 150 L Zymo DNA/RNA Shield. Cells were submitted preserved in Zymo DNA/RNA Shield at ambient temperature for bead based RNA extraction, library preparation and 1 X 90bp  Illumina sequencing with Plasmidsaurus.