RNA was extracted using the RNeasy mini kit from QIAGEN RNA-Seq libraries were prepared from PolyA selected mRNA (eukaryotic) using the NEBNext Ultra II RNA Library Prep Kit for Illumina 24. Multiplexed sequencing was conducted on a NovaSeq 6000  using the NovaSeq 6000 SP Reagent Kit v1.5, paird end to a read depth of 20-30 million per sample.