Bone marrow from femur and tibia of mice was flushed using 1mL of Mg2+ and Ca2+ free PBS (Cytiva cat# SH30256.01). 1.5 ml of histopaque 1077 was pipette on top of histopaque 1119 carefully to preserve the interface between the two layers. Total bone marrow (1mL) was then added on top of histopaque gradient and the tube wa centrifuged at 1000x g for 25 minutes with zero acceleration and zero brake. Once centrifuged, the upper low-density and lower high-density bands of cells were collected and washed with excess PBS. high density band was frozen and used for the RNA sequence protocol RNA libraries were prepared for sequencing using Innomics protocol