Total RNA was extracted using the RNeasy Plus Mini Kit (74134; Qiagen, Hilden, Germany) Libraries were constructed using the TruSeq Stranded mRNA Library Prep Kit (Illumina) according to the TruSeq Stranded mRNA Reference Guide #1000000040498 v00. The process included poly(A)+ RNA purification, RNA fragmentation, reverse transcription to cDNA, adapter ligation, and PCR amplification with size selection for inserts of 200-400 bp.