Immune cells were isolated from single Peyer's patches. Cells from each Peyer's patch were hashtagged with Feature Barcode technology (TotalSeq C #1-#4, BioLegend).  Non-naive and naive B cells were FACS sorted using the FACSAria Fusion (BD) equipped with a 70 mm Nozzle.  Cells were further processed using the Chromium Next GEM Single Cell 5' Reagent Kit v2 according to the manufacturer's instructions (10x Genomics, Inc., United States). The experiment aimed for 5000 recovered cells. Sequencing was performed on an Illumina NextSeq 500.