Total RNA was extracted from the iWAT of HFD group and HEP group using TRIzol® Reagent according the manufacturer's instructions. Transcriptome libraries were prepared using the TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA) with 1 μg of total RNA. Libraries were size selected for cDNA target fragments of 300 bp on 2 % Low Range Ultra Agarose followed by PCR amplified using Phusion DNA polymerase (NEB) for 15 PCR cycles.