80-250 µl of aqueous humour was aspirated from the anterior chamber of 11 consenting patients (Supplementary Table 2) presenting at Oxford Eye Hospital with uveitis. Aqueous humour was kept at 4°C for up to 4 hours before processing for single cell RNA sequencing (scRNAseq). Cells were pelleted by centrifugation at 500xg for 5 mins at RT and cell pellets resuspended in 40 µl of 1% BSA/PBS and loaded onto 10X Genomics Chromium Controller (Chip K). Gene expression and T cell receptor (TCR) sequencing libraries were prepared using the 10x Genomics Single Cell 5' Reagent Kits v2 (Dual Index) following manufacturer's protocol (CG000330 Rev B) and stored at -20°C at 10 nM. Libraries were sent to Novogene (Cambridge, UK) and sequenced on the NovaSeq X Plus platform (Illumina, v1.5 chemistry, 150bp paired end).