Total RNA was extracted from the differentiated duck primary myoblasts using Trizol reagent (Invitrogen, Carlsbad, CA, USA) and depleted ribosomal RNA using the Ribo-Zero rRNA Removal Kit (Illumina, San Diego, USA). Poly(A) RNA sequencing libraries were generated and paired-end (PE150) sequenced using an Illumina Novaseq 6000 System (LC Bio, Hang-zhou, China) according to standard protocols.