DNA was extracted from ticks collected feeding on Lepus californicus. Circular DNA amplified by rolling-circle amplification (RCA) reaction with a phi29 DNA Polymerase Kit (Watchmaker Genomics, USA). An Illumina DNA LP (M) Tag 96 sample preparation kit was used to prepare DNA high throughput sequencing libraries (Illumina, USA). The libraries were sequenced at Psomagen, USA, on an Illumina NovaseqX platform.