Library preparation was performed from 100 ng of genomic DNA using the MGIEasy FS DNA Library Prep Set (MGI Tech) following the standard protocol. Adapters were ligated, DNA ends repaired, fragments circularized, and DNA nanoballs (DNBs) synthesized before flow-cell loading. Sequencing was conducted on a DNBSeq-G400 platform (MGI Tech) using the DNBSeq PE150 configuration.