Prior to euthanasia, mice were fasted for 6 h. Liver tissue was rapidly excised from each mouse and weighed to record any changes in organ weight. Immediately following excision, tissue samples were flash-frozen in liquid nitrogen and preserved in RNAlater Stabilization Solution (ThermoFisher, Waltham, MA).  Total RNA was isolated using TRIzol (ThermoFisher). Barcoded cDNA was prepared from 300ng total RNA utilizing the IsoSeq Express 2.0 Kit (PacBio, 103-071-500) according to manufacturer. Barcoded Kinnex Array libraries were prepared from 56ng equimolar pooled barcoded cDNA according to “Preparing Kinnex Libraries using the Kinnex full-length RNA kit, 103-238-700, Rev03 (MAR2024)” utilizing the Kinnex PCR 8-Fold kit (PacBio, 103-071-600) and Kinnex concatenation kit (PacBio, 103-071-800). 150pM of the library was prepared for sequencing following calculations generated with SMRT Link v13.1 software (PacBio) for Kinnex full-length RNA library type and applications. Briefly, the library was annealed to the Kinnex sequencing primer and bound to polymerase prior to purification with SMRTbell cleanup beads (PacBio, 100-265-900) utilizing the Revio Polymerase Kit (102-739-100, PacBio). Sequencing was performed on a 25M SMRT cell on a Revio platform (PacBio) and data collected over a 24 hour movie with adaptive loading enabled.