At Day 1 post-MGT overexpression, cells were harvested and frozen in 90% FBS with 10% DMSO using a controlled-rate freezing container (Mr. Frosty) at –80C. Approximately 250,000 cells were used per condition. CUTandRUN DNA samples sequencing libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7645L) along with NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1, NEB #E7600S). DNA libraries were cleaned up using AMPure XP reagent, Bechman Coulter, A63881).