Introducing synthetic thermostable RNase inhibitors in single-cell RNA-seq (Bulk RNA-seq libraries)
Cultured mouse tail tip fibroblasts (MTTFs) (CAST/EiJxBL6) were pelleted and stored at -80Â°C prior to RNA extraction. MTTFs were cultured in standard medium, DMEM with 10% FBS, supplemented with 1x non-essential amino acids and 1x sodium glutamate. Bulk RNA was extracted from cultured mouse tail tip fibroblasts using TRIzol Reagent (Invitrogen). Libraries were constructed using commercially available RNA-seq kits TruSeq RNA Library Preparation Kit v2 (Illumina), NEBNext Ultra RNA Library Prep Kit for Illumina (NEB) and QIAseq FX Single Cell RNA Library Kit (QIAGEN) according to the manufacturers instructions.