Total RNA was extracted by VeZol (Vazyme, R411) and 500ng of total RNA was used for the construction of sequencing libraries. RNA fragmentation was performed using the NEB Magnesium RNA Fragmentation Module at 94°C for 5-7 min. The cleaved fragments were then reverse transcribed into cDNA using SuperScript II Reverse Transcriptase (Invitrogen, 1896649), followed by second-strand cDNA synthesis with U-labeling employing E. coli DNA polymerase I, RNase H (both from NEB), and dUTP solution (Thermo Fisher, R0133). PCR amplification yielded a final cDNA library with an average insert size of 300±50 bp. The final RNA library was subjected to 2×150 bp paired-end sequencing (PE150) on an Illumina NovaSeq 6000 platform (Novogene Technology) following the manufacturer's protocol.