Cell pellets were resuspended in 1 mL of lysis buffer (1× PBS, 0.1% SDS, 0.5% sodium deoxycholate, 0.5% NP-40, supplemented with freshly added protease inhibitors), and immunoprecipitations were performed 2 h at 4 °C using a mouse monoclonal anti-FLAG antibody (8 μg conjugated to 100 μL of Protein A Dynabeads). Subsequent radiolabeling, RNA extraction, reverse transcription, and qPCR steps were performed to obtain input cDNA Purified RNA samples were subjected to reverse transcription using barcoded RT primers, enabling the subsequent pooling of samples. To increase the overall yield, cDNA from each replicate of the wild-type and mutant conditions was pooled together following the reverse transcription step. The resulting cDNA was then immunoprecipitated using a BrdU-specific antibody, followed by circularization with CircLigase. The circularized product was then amplified via qPCR. This amplification utilized the KAPA enzyme supplemented with SYBR Green dye and specific Illumina indexing primers, DP5-PE and SP3-PE. Amplification continued until the fluorescence reached 400 Relative Fluorescence Units (RFU). The resulting amplicons were purified using AMPure magnetic beads. Finally, the pooled libraries were sequenced on the Illumina NextSeq 2000 platform.