RNA-Seq of CRISPR/Cas9 zebrafish somatostatin 6 (sst6) mutant embryos at 0, 3 and 6 hours post fertilization compared to wild type controls
recently fertilized (0), 3 and 6 hours post fertilization embros were collected into RNA later Total RNA was extracted using the TRIzol reagent (Invitrogen, CA, USA) according to the manufacturer's protocol. RNA purity and quantification were evaluated using the NanoDrop 2000 spectrophotometer (Thermo Scientific, USA). RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) Ribo-off rRNA Depletion Kit（vazyme, Nanjing, China）was used to remove ribosomal RNA, then the libraries were constructed using VAHTS Universal V6 RNA-seq Library Prep Kit according to the manufacturer's instructions.