Genomic DNA was extracted from Aeromonas hydrophila isolate FO129-27, collected from spleen and kidney of Colossoma macropomum in Iquitos, Peru. The bacterium was cultured on TSA at 28C for 24 h. DNA libraries were prepared using Illumina DNA Prep (Tagmentation) kit and sequenced on an Illumina MiSeq platform with paired-end 2  150 bp reads.