Use Qubit3.0 DNA detection kit to accurately quantify genomic DNA and determine the amount of DNA that should be added to the PCR reaction. After per, the library size was detected by 2% agarose gel electrophoresis. In order to obtain uniform clustering effect and high-quality sequencing data, the library concentration was determined by Qubit3.0 fluorometer, and the final sequencing library was sequenced by Illumina MiSeq platform.