Per replicate, 500 SAMs or IMs were collected on ice, then ground with a metal ball in a TissueLyzer LT (Qiagen). CTAB DNA extraction was used on the remaining powder. DAP-seq libraries were prepared according to Bartlett et al., (2017) with Illumina TruSeq barcodes. 100 ng of gDNA was used as starting material. Libraries were amplified using 15 cycles. Post-amplification libraries were size selected at >200 bp in length using AMPure XP beads.