RNAseq of CD19-CAR-T cells generated in presence or absence of vitamin C
CD19-CAR-T cells were generated from human primary CD3+ T cells of three donors in presence or absence of vitamin C. RNA was isolated after 24h of co-culture with Nalm-6 cells. Details of experimental design can be found in the M&M section of the manuscript.  RNA was isolated using the RNeasy Plus Kit (Qiagen)  The RNA sequencing library was generated from 500 ng total RNA using the Dynabeads® mRNA DIRECT™ Micro Purification Kit (Thermo Fisher Scientific) for mRNA purification, followed by the NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England BioLabs) according to the manufacturer's protocols. 