Cells previously transfected with siRNA or treated with pharmacologic compounds were collected in RLT plus lysis buffer supplemented with 10uL/mL Beta-mercaptoethanol (ß-ME) and passed through QIAshredder (Qiagen) column. RNA was then extracted using the RNeasy Plus mini kit (Qiagen). Libraries were prepared with 10-500ng input to the QuantSeq mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen) following the manufacturer's protocol. Libraries were quantified with KAPA Library Quantification Kit Illumina® Platforms (Roche) and sequenced on NovaSeq X+ with ~30 M paired-end, 150 bp reads per sample.