SMARTPool siNT and siNr1h2 siRNA (Horizon Discovery, D-001206-13, M-042839-01) were used for Nr1h2 knockdown in mouse embryos. Capillary glass tubes (outer diameter 1 mm, inner diameter 0.8 mm, and length 10 cm) with cut diameter 30–70 μm were used. 4–5pL of 100 μM siRNA was injected into fertilized one-cell embryos. Injected embryos were then transferred into a droplet of M16 medium covered with mineral oil in a 30 mm dish, and cultured until morula embryo stage before harvest. Morulae samples were processed for RNA-seq library preparation using SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing and Nextera XT DNA Library Preparation Kit, following manufacturers' instructions.