Total RNA Isolation Kit (Applied Biosystems, A27828) according to the manufacturer's protocol. Purified total RNA was quantified and validated with nanodrop and fragment analyzer (Agilent, DNF 471 Standard Sensitivity RNA Analysis Kit, 15 nt). cDNA was synthesized using 100 ng of total RNA. cDNA library was prepared using Illumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus kit. Quality control and quantification for amplified cDNA libraries and final sequencing libraries were performed using a Qubit High Sensitivity ds DNA and Agilent Bioanalyzer DNA High Sensitivity Chip analysis. Libraries were normalized to 4 nM concentration and pooled. The pooled libraries were paired end sequenced on NovaSeq SP platform.