Intermittent fasting promotes remodeling of neural and vascular networks in visceral white adipose tissue
Perigonadal white adipose tissue was dissected from mice after cervical dislocation while under isoflurane euthanasia. Tissue was immediately frozen in liquid nitrogen after dissection Adipose samples were lysed using TRIzol reagent. Total RNA was extracted using the Direct-zol RNA MiniPrep kit (Zymo Research) cDNA libraries were generated using the Nugen Ovation SPIA chemistry with a fully automated Wafergenâ€™s Apollo liquid handler