The plants used in this work consisted of genotypes with different ploidy levels and reproductive modes . Victoria (2x) and OTA-S (4x) were used as sexual genotypes, while the  apomictic genotypes were grouped by its different ploidy levels: five tetraploids (Bahiense cv, Ermelo cv, Morpa cv, Tanganyika USDA cv, Tanganyika INTA), the hexaploid Don Pablo, the heptaploid Don Juan, and the octoploid Don Eduardo. These genotypes have been maintained at CERZOS-CONICET, located in Baha Blanca, Buenos Aires, Argentina (3843?0?S, 6216?0?W). The study was conducted in a climate-controlled greenhouse at 25  4C with natural light conditions. Three biological replicates for each genotype were produced by tiller propagation. Leaf tissue was collected for all triplicates  and immediately frozen in liquid nitrogen and stored at -80C until further use. A total of thirty leaf samples (10 genotypes x 3 biological replicates) were used for RNA sequencing.