Femurs and tibias were dissected from Cxcl12-Cre;ZsGreen mice of 24-week-old. The proximal and distal metaphyses of the femurs and tibias were excised, and the diaphyseal bone tissues were immersed in Hank's balanced salt solution (HBSS). The collected tissues were enzymatically dissociated by incubation at 37 ºC for 60 min in HBSS containing 0.2% Collagenase and 0.2% Dispase. The isolated cell suspension was filtered through a 70 μm cell strainer, followed by red blood cell lysis using RBC Lysis Buffer. After washing with HBSS, the cell suspension was adjusted to 1.0 × 10⁷ cells/ml in HBSS. ZsGreen-positive cells were isolated using a BD FACSAria™ III cell sorter, and the sorted cells were resuspended in HBSS to a final concentration of 1.0 × 10⁷ cells/ml. The sorted cell suspension was processed using the 10x Chromium X system with Chromium GEM-X Single Cell 5' v3 reagents, according to the manufacturer's instructions. Subsequently, reverse transcription for first-strand cDNA synthesis was performed within the GEMs to generate a single-cell library with individually barcoded cDNAs for each cell. The single-cell library was sequenced using NovaSeq X Plus.