PBMCs were isolated from freshly collected blood using Histopaque 1077 (Sigma-Aldrich) following a standard density gradient procedure. Briefly, blood was collected using BD Vacutainer ACD tubes and immediately processed. After transferring to a centrifuge tube, the blood was diluted 1 in 3 with PBS, and Histopaque 1077 was gently pipetted at the bottom of the tube. After centrifugation, the mononuclear interphase was collected, rinsed with PBS and cryopreserved. iPSC lines were generated from the PBMCs by a Sendai reprogramming method using the Cytotune 2.0 kit (Life Technologies) following the manufacturer recommendations.