Splenic B cells were isolated from WT and NEMODK mice, stimulated with anti-CD40 and IL-4 for two days, then washed and resuspended in wash buffer. Cells were bound to ConA-coated beads, incubated overnight with anti-RelA (Cell Signaling, 8242), anti-H3K4me3 (Active Motif, 39159), anti-H3K27ac (Active Motif, 39034), and anti-H3K27me3 (Cell Signaling, 9733) antibodies, followed by secondary incubation with anti-rabbit IgG antibodies (Antibodies Online). After washing, the cells were incubated with the pA-Tn5 adapter complex (Epicypher) at room temperature for 1 hour. Tagmentation was performed at 37°C, after which DNA was extracted and PCR-amplified to generate sequencing libraries. Libraries were sequenced on an Illumina NovaSeq platform to produce paired-end 150 bp reads.