Cells were dissociated using 1x TrypLE Select (Thermo Fisher Scientific #12563011). The cells were then resuspended in PBS supplemented with 1% BSA. Single-cell RNA sequencing libraries were prepared according to the manufacturer's instructions using the 10x Genomics Chromium Single Cell 3' v2 Reagent Kit. Briefly, single-cell suspensions were loaded onto the Chromium Controller to encapsulate individual cells into nanoliter-scale Gel Beads-in-Emulsion (GEMs), where cell lysis and barcoded reverse transcription occurred. Each GEM contained a unique 10x barcode, a unique molecular identifier (UMI), and poly(dT) primers to capture mRNA molecules. Following reverse transcription, cDNA was amplified using PCR and size-selected for optimal library construction. Sequencing libraries were prepared by enzymatic fragmentation, end-repair, A-tailing, adaptor ligation, and sample index PCR. Libraries were quantified using TapeStation (Agilent) and pooled for sequencing.