ApoE isoforms differentially alter baseline antioxidant levels critical to ferroptosis mitigation
5 million cells were homogenized in TRIzol reagent using a BeadBug Benchtop Homogenizer. Brain tissue was suspended in 1 mL of TRIzol and homogenized for 6 rounds of 10 seconds homogenization and 60-second holds between each round. Following homogenization, 300 µL of chloroform was added, and samples were centrifuged with heavy gel phase-lock tubes (VWR, 10847-802) to separate the aqueous phase. RNA purification kit (Quantabio, Extracta Plus, Cat# 95214-050) was used following the manufacturer's protocol. RNA concentration and integrity were assessed with a Qubit 4.0 Fluorometer and Agilent Bioanalyzer prior to sequencing.  Library preparation (mRNA library, poly A enrichment) was performed by Novogene.