Total RNA was extracted from samples. mRNA with poly(A) tails was enriched using oligo(dT) magnetic beads. Purified mRNA was fragmented randomly, followed by first-strand cDNA synthesis with random primers and second-strand cDNA synthesis incorporating dUTP for strand-specific library construction. After end repair, A-tailing and adapter ligation with unique dual index adapters, fragments were purified via AMPure XP beads and amplified by PCR. Library quality was verified using Agilent 2100 Bioanalyzer, quantified by qPCR, and sequenced on Illumina NovaSeq 6000 platform with PE156 strategy by Novogene.