Total RNA was extracted using PureXtract RNAsol RNA isolation solution (GenDepot). Total RNA was enriched by oligo (dT) magnetic beads and library was prepared using KAPA Stranded RNA-Seq Library Prep Kit (Illumina). Completed library were quantified with Agilent 2100 Bioanalyzer and sequenced on the Illumina NovaSeq 6000 instrument.