Single cells were sorted into 3µL of BioSkryb ResolveDNA Cell Buffer were used. PTA reactions were assembled by sequential addition of proprietary BioSkryb reagents. Amplified DNA was purified using the BioSkryb ResolveDNA Bead Purification Kit. DNA yield and fragment size (200–4,000 bp) were confirmed by Qubit HS dsDNA assay and Agilent TapeStation (HS D5000 ScreenTape). 4 loci QC-pass PTA products (≥500 ng input) were converted into PCR-free Illumina libraries using the KAPA HyperPlus Library Prep Kit, following the manufacturer's protocol. For all ResolveOme reactions, 100ng of purified DNA fraction and 20ng of purified cDNA was carried forward to the library preparation steps and standard protocols were followed as per BioSkryb library preparation kit protocols. PTA libraries were quantified by Qubit and TapeStation. pooled equimolarly, and sequenced on an Illumina NovaSeq X PLUS platform (150 bp paired-end reads, target 30× genome coverage per sample).