The total RNAs were isolated using the Trizol reagent (Invitrogen) following the manufacturer's protocol. RNA quality was inspected using Nanodrop (all samples had 260/280-ratio≥1.9). Then libraries with different indexs were multiplexed and loaded on an Illumina NovaSeq 6000 instrument for sequencing using a 2x150 paired-end (PE) configuration according to manufacturer's instructions.