Transcriptomic analysis of the interactions between LINC00654 and HuR
OVCAR3 cells were purchased from the Institute of Biochemistry and Cell Biology of the Chinese Academy of Sciences (Shanghai, China). OVCAR3 cells were supplemented with 20% fetal bovine serum. Cells were harvested 48 hours post-transfection for subsequent experiments, including transfection efficiency and functional assays. Total RNA was extracted from cells and tissues using the RNA isolator Total RNA Extraction Reagent (Vazyme, Nanjing, China) according to the manufacturer's instructions. RNA was then reverse-transcribed into cDNA using the HiScript III RT SuperMix qPCR kit (Vazyme).