Single-cell suspensions with viability greater than 90% were resuspended at a density of 1,000 cells/μL. Approximately 8,000–10,000 cells per sample were encapsulated into individual droplets using the 10x Genomics Chromium Single Cell-A Chip platform. Single-cell RNA-seq libraries were prepared using the Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (10x Genomics) following the manufacturer's protocol. Sequencing was performed on an Illumina NovaSeq 6000 platform, generating 150 bp paired-end reads with a target sequencing depth of ~150 million reads per sample.