Suprarenal aortae were perfused with saline and periaortic tissues removed. Samples incubated in RNAlater (AM7020, Invitrogen) for 1 hr. mRNA extracted using RNeasy Fibrous Tissue Mini Kit (74704, Qiagen). mRNA libraries constructed following poly-A enrichment with poly-T oligo-attached magnetic beads. First-strand cDNA synthesized using random hexamer primers, followed by second-strand synthesis. Libraries prepared after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. Quality assessed by Qubit and bioanalyzer.