The main reference of DAP-seq experimental process is: Bartlett A, O'Malley RC, Huang SC, et al.,  Mapping genome-wide transcription-factor binding sites using DAP-seq. Nature Protocl, 2017. (1) DNA was extracted from 10-day rice seedlings, then genomic DNA was broken into ~200bp fragments, and the DNA library was prepared by adding the linker. (2) The expression vector containing the target protein coding sequence was constructed and the Halo-target protein fusion protein was expressed in vitro. The HaloTag-TF fusion protein was incubated with the DNA library, the DNA was recovered, and the second generation library was constructed after PCR amplification.