The abdominal  vena cava was cannulated with the thoracic vena cava clamped. The portal vein was snipped and 20 mL HBSS -/- buffer was perfused. Then, HBSS +/+ with 4 mg/mL Liberase was perfused until the liver was digested to acheive a single-cell suspension. Cells were washed with HBSS -/- and incubated with DAPI. Cells were sorted on FACAria II into DMEM supplemented with 10% FBS. Cell partitioning into Gel Beads-in-emulsions (GEMs) and barcoding was carried out using the Chromium Controller, following the manufacturer's protocol. Barcoded cDNA libraries were prepared from GEMs for each mouse, and sequencing was performed on an Illumina HiSeq X platform (PE150).