FACS sorted Lin-TdTomato+ neoplastic cells from KT;H11LSL-Cas9 mice transduced with sgRNA. Immediately after FACS, the sorted neoplastic cells were fixed with Evercode Cell Fixation kit (Parse Biosciences, ECF2101). The fixed cells are processed with Parse Biosciences scRNA-seq protocol. Parse Biosciences scRNA-seq protocol