CeleroTM DNA-Seq kit (NuGEN, San Carlos, CA) has been used for DNA library preparation following the manufacturers instructions. Both input and final library were quantified by Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA) and quality tested by Agilent 2100 Bioanalyzer High Sensitivity DNA assay (Agilent technologies, Santa Clara, CA). Libraries were then prepared for sequencing and sequenced on a NovaSeq 6000 instrument (Illumina, San Diego, CA, USA) with a 2x150 bp sequencing layout with a target throughput of 80 millon reads per sample.