Single daughter cells after division of ex vivo cultured primary HBC cells were collected individually using mouth pipette with pairing information noted. Each cell was lysed and 1 ml of pre-diluted (1:10^6) ERCC spike-in was added to the single cell lysate. RNAs inside single cell lysate were captured by oligo-dT beads and then processed following the G&T-seq protocol to construct the single cell RNA-Seq library.