Library preparation using the Illumina AmpliSeq Cancer Hotspot Panel v2 involved using 10 ng of genomic DNA. The DNA was first quantified and normalized, followed by multiplex PCR to amplify regions of interest across key cancer genes. After amplification, the amplicons were digested and ligated with sequencing adapters, including unique indexes for sample identification. The libraries were then purified, quantified, and pooled for sequencing