After RNA-ChIP, RNA was purified using TriPure Isolation Reagent (Millipore-Sigma). To remove residual DNA, the purified RNA was treated with DNase I, followed by further purification using the RNA Clean & Concentrator-5 Kit (Zymo). A total RNA library was then prepared using the Illumina Stranded Total RNA-seq Library Prep Kit with RiboZero Plus. Samples were pooled and sequenced on NextSeq 2000 P2 using paired-end sequencing.