mRNA was purified from total RNA using poly-T magnetic beads. Purified mRNA was fragmented into 400500 bp, reverse-transcribed into cDNA, and converted to blunt-ended fragments with 3 adenylation. Illumina PE adapters were ligated, followed by size selection (AMPure XP beads) and 15-cycle PCR enrichment. Libraries were quantified using Agilent High Sensitivity DNA Assay and sequenced on the NovaSeq 6000 platform (Illumina).