Genomic DNA from conidia digested with NlaIII and HpyCH4IV, annealed to adapters with variable-length barcodes, and pooled in sets of 48 samples. Size selection, then PCR done on each pool of 48 samples to add adapters with unique indexes. Combined index pools in equimolar amounts into a final pool, then narrow-range size selection, and sequencing on Illumina NovaSeqSP.