Response of Klebsormidium treated with glutamine and GS-inhibitors
Samples were harvested via centrifugation, washed once in sterile MilliQ water, and snap-frozen in liquid nitrogen Cell suspensions were precultured in liquid Full Yamagami media for seven days prior to treatment Klebsormidium cultures were inoculated to liquid Full Yamagami media (pH5.5, 1% sucrose) containing the chemical of interest and grown for 48h at 80rpm, at 22Â°C, under 24h of low light. Frozen samples are milled in extraction buffer and total RNA extracted using the QIAGEN Plant RNeasy kit Directional mRNA library preparation (poly A enrichment)