Genomic DNA (gDNA) from 2  10^8  L. mexicana cells was isolated using the phenol/chloroform method. DNA integrity was confirmed via 1% agarose gel electrophoresis. Samples were purified with the Short Read Eliminator XS kit (PacBio) and quantified using a Qubit 4 Fluorometer (Thermo Fisher Scientific). Approximately 1 g purified gDNA was used for nanopore sequencing library construction with the Native Barcoding Kit 24 V14 SQK-NBD114.24 (Oxford Nanopore Technologies) and sequenced on a PromethION Flow Cell.