1 microL of genomic DNA was used to construct a WGBS library with the EZ DNA Methylation-Gold Kit DNA methylation reagent kit method and protocol. DNA was fragmented, end repaired, and daapters were ligated. A's were added to the 3' ends, fragments were size-selected and samples were bisulphite-treated. PCR amplification was used to generate the WGBS library, library quality was assessed with a Qubit 3.0 and the CFX96 Touch Real-Time PCR Detection System was used. Pooled qualified libraries were sequenced on an Illumina NovaSeq 6000 sequencing platform