The pET-22b-LORE plasmid constructed by Qingke Biology was verified by Sanger sequencing. The target fragment, namely the LORE gene, was inserted into the vector backbone. Plasmid DNA was extracted from a single colony and sequenced in one direction using gene-specific primers to confirm the correct sequence and absence of unexpected mutations. The sequencing library was derived from synthetic plasmid DNA and was specifically designed for the verification of the cloned insertion fragment.