10x used: Chromium Nest GEM single Cell 3' Reagent Kits V3.1 (Dual Index). Drop-seq used: McCarroll laboratory publised protocol (34. Macosko,E.Z., Basu,A., Satija,R., Nemesh,J., Shekhar,K., Goldman,M., Tirosh,I., Bialas,A.R., Kamitaki,N., Martersteck,E.M., et al. (2015) Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets. Cell, 161, 1202-1214) Seurat scRNA-seq