Nuclear RNA was isolated from primary rat microglial subcellular fractions following feeding conditions using Trizol and phenol-chloroform extraction. mRNA was subsequently purified using the PureLink RNA Mini Kit (Thermo Fisher Scientific, 12183018) with on-column DNase treatment. RNA samples underwent post-DNase digestion to minimize genomic DNA contamination prior to library preparation. RNA integrity and concentration were assessed using the RNA ScreenTape system (reagent: Agilent, #5067-5577, RNA ScreenTape: Agilent, 5067-5576, TapeStation: 4200 TapeStation System, Agilent) and NanoDrop spectrophotometry. Only high-quality RNA (RNA integrity number (RIN) > 7) was used for downstream applications. RNA libraries were prepared from purified nuclear RNA using ERCC RNA Spike-In Mix controls using QIAseq® UPXome Low Input Kit (334705) and sequenced on the Illumina NovaSeq platform (SP flow cell configuration) using paired-end 150 bp sequencing read configuration.  UPX-002 ACCAGACTTG-CGATGAGCAC No Depletion pool UPX-003 ACTGGCGAAC-GATAAGTCGA Pool 1 1-12 UPX-004 GCGTTAGGCA-TCACGCCTTG Pool 2 13-24 UPX-005 TTATCGGCCT-AGGAACACAA Pool 3 25-35