Total RNA was isolated from 100 mg of frozen muscle tissue using  TRI-Reagent (Sigma-Aldrich, St. Louis, MO) according to the manufacturer's protocol Sequencing Libraries were prepared using LIB_PREP_PROTOCOL. READ_TYPE, 75bp single reads were sequenced on NUM_LANES lane(s) of an Illumina ILLUMINA_INSTRUMENT. The output was ~16 million reads per sample.