7 d post injury, lumbar L1-L5 DRG was collected from the injected side. DRG were minced and digested with 25U/mL Papain. Then subjected to three sequential enzymatic digestions with a mixture containing 1 mg/mL collagenase type I (1 mg/ml; Worthington Biochemical Corporation, Lakewood, NJ, USA, LS004197), 1.2 mg/mL dispase II (Sigma, T8003).  The distal femur epiphysis including the surrounding tissue of injured growth plate was carefully dissected and subjected to five sequential enzymatic digestions with collagenase type I/II (1 mg/ml), with each digestion for 10 mins. Then cell fractions (from sequential digestions 1 to 5) were collected and subsequently filtered through 40-μm sterile strainers for all samples, 10x Genomics Chromium standard protocol