Illumina adapters were added to 50 ng of amplicon DNA using the Ovation Ultralow System V2 1-96 kit (NuGen Technologies, Inc.). Amplicons were pooled in equal volume ratio. An aliquot of the pool was run on a MiSeq (Illmina, Inc.) using a MiSeq Reagent Nano kit, ver2. This QC run consisted of 25 cycles followed by an index read. The pool was then rebalanced based on the percentage of reads seen for each amplicons index. The final pool was then sequenced on the HiSeq 2500 in Rapid mode using version 2 chemistry to generate a minimum of 11 M paired-end 300 base reads per amplicon. Post-run processing of data was performed using RTA 1.18.64 and CASAVA 1.8.2.