Single neurons from the trigeminal ganglion were isolated for sequencing. RNase inhibitor and barcoded oligo-dT SMART primers were added, followed by reverse transcription to incorporate SMART sequences. cDNAs were pooled, purified, amplified, QCed, sheared, end-repaired, A-tailed, and ligated with Illumina adapters. Libraries were indexed, amplified, and sequenced as paired-end 50-base or 26x98 reads with 20% phiX, capturing cell barcodes, plate indices (if applicable), and 3' mRNA data.