DNA was extracted using a commercial nucleic acid purification kit (cw-bio). Library preparation was carried out using the PresPrep DNA Library Preparation Module and PresPrep Hybrid Capture Reagents. Enrichment of exonic regions was achieved using the CapTruth Human Exome X plus Panel. Paired-end sequencing (2x150 bp) was conducted on the GeneMind SURFSeq5000 platform. Bioinformatic analysis followed a standardized pipeline. Raw sequencing reads were quality-trimmed and adapter-removed using fastp (v0.23.1). Clean reads were aligned to the human reference genome (GRCh37/hg19) using BWA-MEM2 (v2.2.1). Somatic single nucleotide variants (SNVs) and small insertions/deletions (InDels) were detected using GATK Mutect2 (v4.2.0.0). As a key addition, copy number variation (CNV) analysis was systematically performed using CNVkit (v0.9.9). All identified variants were functionally annotated using ANNOVAR and SnpEff, with annotation sources including dbSNP, gnomAD, COSMIC, and ClinVar databases.