Genomic DNA was quantified using Qubit dsDNA quantification broad range assay (Thermo Fisher Scientific). DNA size was determined using the Femto Pulse System (Agilent). A total of 3 libraries was prepared per sample to be sequenced on 3 flow cells. DNA was sheared using Covaris g-tubes (Covaris 520079) to achieve an average size of 20-25 kb for DNA from cell lines and 15-20 kb for DNA from tissues. Sheared DNA was size selected on the PippinHT instrument (Sage Science) using the 6-10 kb or the 15-20 kb High-Pass definition. ONT libraries were prepared using the SQK-LSK114 kit following the manufacturer's instructions. Final libraries were eluted in 27 µL of the ONT elution buffer and quantified using the Qubit dsDNA quantification broad range assay. Libraries were loaded at 15 fmoles to be sequenced on R10.4.1 flowcells.