Two libraries were prepared as positive and negative methylation controls using the Human Methylated & Non-Methylated (WGA) DNA Set (Zymo Research, D5013). Libraries were sheared to 8 kb and sequenced using the Ligation Sequencing Kit V14 standard protocol (Oxford Nanopore Technologies, SQK-LSK114) with reagents from the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing (New England Biolabs, E7180S). Libraries were quantified using a Qubit 4 fluorometer (ThermoFisher Scientific, Q33238) with Qubit dsDNA HS (ThermoFisher Scientific, Q33230). 150 ng of each sample was loaded onto PromethION (Oxford Nanopore Technologies, PRO-SEQ048) flow cells (Oxford Nanopore Technologies, FLO-PRO114HD). Runs were stopped after 72 hours.