To further explore the mechanism of Erastin in TNBC, we conducted transcriptome sequencing experiments in MDA-MB-231 cells in response to 48h treatment with Erastin (20uM). RNA-seq data revealed that 365 genes were upregulated and 614 genes were downregulated in Erastin treated cells. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that TNBC cells death induced by Erastin related to TNF signaling pathway, Hematopoietic cell lineage, Toll-like receptor signaling pathway and ferroptosis.