ChIP-seq  against RUNX2, RNA-Polymerase II and Histone Marks (H3K27ac, H3K4me1, H3K4me3) in human Papillary Thyroid Cancer cell line (MDA-T41)
20M cells were collected for Chromatin Immunoprecipitation. MDA-T41 cells were grown in RPMI-1640 supplemented with 1% non-essential amino acids, 10% FBS and 1% penicillin-streptomycin. All cell lines were grown at 37Â°C/ 5% CO2 and routinely tested for mycoplasma infection. ChIP samples were purified with PCR Purification Kit (Qiagen) and eluted in 50ul of Elution Buffer. Libraries were obtained starting from 3ng of ChIP and Input samples following ThruPLEX DNA-Seq Kit (Takara Bio Ink) protocol.