scRNAseq of CD19-CAR-T cells generated in presence or absence of vitamin C
CD19-CAR-T cells were generated from human primary CD3+ T cells of three donors in presence or absence of vitamin C. Viable CD3+ T cells were sorted after 24h of exposure to CD19+ B-LCLs.  S01: CD19 CAR T cells pre exposure to CD19+ B-LCLs. HTO capture antibodies were used to allow discrimination between CD19 CAR T cells generated in presence or absence of vitamin C, obtained in three independent experiments (donors). HTO1: donor 1, 0 µM vitamin C HTO2: donor 1, 50 µM vitamin C HTO3: donor 2, 0 µM vitamin C HTO4: donor 2, 50 µM vitamin C HTO5: donor 3, 0 µM vitamin C HTO6: donor 3, 50 µM vitamin C  S02: CD19 CAR T cells post exposure to CD19+ B-LCLs. HTO capture antibodies were used to allow discrimination between CD19 CAR T cells generated in presence or absence of vitamin C, obtained in three independent experiments (donors). HTO1: donor 1, 0 µM vitamin C HTO2: donor 1, 50 µM vitamin C HTO3: donor 2, 0 µM vitamin C HTO4: donor 2, 50 µM vitamin C HTO5: donor 3, 0 µM vitamin C HTO6: donor 3, 50 µM vitamin C Sorted cells were subjected to the 10x pipeline. More details see \"nucleid acid library construction protocol\". Library preparation for single cell mRNA-Seq analysis was performed according to the Chromium NextGEM Single Cell 5ʹ Reagent Kits v2 CellSurfaceProtein User Guide (Manual Part Number CG000330 Rev E; 10x Genomics). According to the protocol a given excess (100%) of cells was loaded to the 10x controller. Fragment length distribution of generated libraries was monitored using 'Bioanalyzer High Sensitivity DNA Assay' (5067-4626; Agilent Technologies). Quantification of libraries was performed by use of the 'Qubit® dsDNA HS Assay Kit' (Q32854; ThermoFisher Scientific).