Testicular cells were placed in centrifuge tubes, and total RNA was extracted by A and placed in a -80°C refrigerator, and RNA-seq libraries were created following the protocol provided by the manufacturer RNA-seq: RNA-seq was constructed according to the instructions of the NEBNest® UltraTM Directional RNA Library Prep Kit for Illumina (NEB), utilizing the Illumina HiSeq 4000 platform for bipartite sequencing, with three biological samples, each containing 74 million paired reads