B-2 and B-1a cells from the peritoneal cavity of Elavl1fl/fl and Elavl1fl/fl Mb1Cre mice were isolated by FACs sorting. B-2 cells - CD19+ B220hi CD43- CD5- CD11blow and B-1a cells - CD19+ B220low CD43+ CD5+ CD11bhi. Total RNA was isolated using a RNeasy Micro Kit from Qiagen libraries generated using the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (New England BioLabs). NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1 or 2) mRNA-seq library and PE 100 bp sequencing using a DNBSeq platform