Perturbational sequencing of patient tumors reveals midkine as an innate immune checkpoint in neuroendocrine tumors
Control and IFNb treated cells were harvested with trypsin. 10x6 MCC13 MDK-WT and MDK-KO tumor cells were plated per 12-well plate of MCC13 MDK-WT and MDK-KO.  Cells were treated with 1000 IU/ml IFNb for 24 hours. RNA was harvested using Rneasy mini kit (Qiagen).  1 ug of RNA was submitted for construction of sequencing library.