Primary astrocytes were plated at 1x10^5 cells per well in 2 mL in 6 well plates. One day after plating 2 uL of Dexamethasone (100 nM) was added to each well. One day after Dexamethasone treatment LPS/IFN (100 ng/mL and 100 U/mL) was added and incubated for 24 hours. Following incubation samples were lysed with the Qiagen RNeasy Mini Kit according to the manufacturer's protocol.