eCLIP-seq was used to map PEG10 binding sites in HepG2 cells. UV-crosslinked cells were lysed and RNase-treated to fragment RNA. PEG10-RNA complexes were immunoprecipitated with PEG10 antibody. RNA was ligated with 3' and 5' adapters, reverse transcribed, and PCR amplified to construct sequencing libraries, followed by Illumina paired-end sequencing.