Temporal Control of Macrophage Pro-inflammatory Phenotype by a Biphasic HIF-1α Regulatory Program
RNA samples from bone marrow-derived macrophages (BMDMs) from unstimulated (control) and M(LPS+γ) macrophages after 6 hours and 24 hours of activation in Hif1a+/+ and Hif1a-/- macrophages were used for the experiment. BMDMs were lysed for RNA extraction using the RNAeasy kit (Qiagen). RNA integrity was analyzed on a Bioanalyzer RNA Pico 6000 chip at the Core Facility for Scientific Research – University of São Paulo (CEFAP-USP).  Complementary DNA library construction and sequencing were performed by BGI Global Genomics Services using the DNBSeq platform.