Total RNA was extracted with the help of RNAprep pure plant plus kit. The quality and quantity of each RNA sample were assessed by the NanoDrop 2000 and the Agilent 2100 Bioanalyzer. Only RNA samples with absorption 260/280 ratios from 1.9 to 2.2, 260/230 ratios from 2.0 to 2.5, and RNA integrity number values