Asexual P. falciparum parasites were cultivated at 5% hematocrit in O+ human erythrocytes (Interstate Blood Bank, Inc.). All cultures were maintained with regular media changes under 5% O2, 5% CO2, 90% N2 at 37 C. Standard, nutrient-rich propagation used RPMI 1640 medium supplemented with 25 mM HEPES, 31 mM NaHCO3, 0.37 mM hypoxanthine, 10 g/mL gentamicin, and 0.5% w/v NZ Microbio BSA (MP Biomedicals). DNA was isolated from parasite cultures using Quick-DNA Miniprep kit (Zymo Research) according to the manufacturers protocol. DNA was used for standard PacBio DNA library prep and sequencing on the Sequel II