Collect the cells into EP tube, add TRIZOL to fully lyse the cells, then centrifuge, transfer the supernatant to a new EP tube, add chloroform, fully shake, stand for 10min, and centrifuge again.Transfer the supernatant to a new EP tube, add isopropanol to assist precipitation, discard the supernatant, wash the precipitation twice with 75% absolute ethanol, and finally dissolve the precipitation with enzyme-free water to obtain total RNA. Reverse transcription of total RNA into cDNA. The RNA-seq libraries were sequenced on a BGIseq500 platform to generate paird-end 150 bases reads