Bulleidia sp. obtained from stool specimens of human colon cancer patients, was cultured in GAM medium at 37C in an anaerobic condition for 24 hours. The genomic DNA was extracted from the harvested cells using NucleoBond HMW DNA (MACHEREY-NAGEL) according to the manufacturers protocol. A nanopore sequencing library was prepared using the Rapid Barcoding Sequencing Kit (SQK-RBK114) (Oxford Nanopore Technologies) and sequenced using an R10.4.1 Flowcell (FLO-MIN114) on a GridION X5 device (GridION software release 23.07.12; Oxford Nanopore Technologies). Base calling (Super Accurate Mode) and demultiplexing were performed using Guppy v.6.5.7 software, resulting in 177,068 reads of N50 length 17,846 bp, totaling 1.577Gbp.