The RNA was extracted from the microdissected tissue using the Qiagen all prep DNA/RNA micro kit (80284) following the supplied instructions. The RNA integrity numbers were determined by TapeStation high sensitivity RNA analysis (5067-5579, 5067-5581, 5067-5580, Agilent) and all used samples had a RNA integrity numbers (RIN) between 7 to 9. RNA-seq library construction was performed by BGI using their standard RNAseq protocol and Illumina high seq 4000. Quality control checks and barcode removal according to BGI protocol.