Total RNA was purified from 2025 pairs of testes from 6-8-day-old males using the Nucleospin RNA purification kit (Macherey-Nagel). Samples were sent to Macrogen for library preparation and sequencing. Duplicate TruSeq Stranded mRNA libraries, corresponding to two biological replicates per parental species, hybrids and back-crosses. Finally, sequencing was performed for 151 bp paired-end reads using the Illumina NovaSeq 6000 technology. We obtained 6686 million paired-end reads for each sample, resulting in a total of 1,110 million paired-end reads.