RNA samples (n=32) were assessed for quality using the Agilent 2100 Bioanalyzer and 2200 TapeStation, ensuring RIN values 7. Libraries were prepared with the SureSelectXT RNA Direct Reagent Kit for strand-specific RNA-Seq. Sequencing was performed on the Illumina NovaSeq 6000 platform with paired-end reads (2  101 bp) using Illumina's SBS technology. Data quality was evaluated with metrics such as Q20/Q30 scores and GC content, achieving an average Q30 >95%.