1 microg mRNA was adjusted to 10 ng/microl in 100 microl and fragmented using a BioRuptor ultrasonicator (Diagenode) with 30 s on/off for 30 cycles. 10% of the fragmented mRNA was saved as input. m6A IP was performed using the EpiMark N6-Methyladenosine Enrichment Kit (NEB) following the manufacturer's protocols. Library preparation was performed using the VAHTS Universal V8 RNA-seq Library Prep Kit (Vazyme Biotechnology) according to the manufacturer's protocols. submitted_instrument_model: Illumina HiSeq X Ten