Short-read RNA sequencing was performed on the Illumina HiSeq platform (paired-end, 150 bp), and full-length transcriptome sequencing was performed on the PacBio Sequel system. For PacBio sequencing, samples comprised seeds, stems, leaves, roots, and shoots of smooth bromegrass. Seed samples were prepared by pooling five seeds from 16, 23, and 33 DAA.