RNA was isolated from menstrual fluid plasma-derived sEV using TRIzol LS (Invitrogen, USA) on a 60 uL sEV sample volume. RNA concentration was assessed with a NanoDrop One (Thermo Fisher Scientific, USA). A minimum of 250 ng RNA was used for RNA sequencing (RNA-Seq). RNA-Seq was performed by Azenta Life Sciences (South Plainfield, NJ, USA). Briefly, library preparation was performed with rRNA depletion, and sequencing was carried out on an Illumina next generation sequencing platform with paired-end 150 bp reads.