Total RNA extraction was carried out as described in (Albacar et al., 2023, PMID: 37013612 ) from three independent cultures of wild-type, pho4(A) (strain PJA-004), pho4(B) (strain PJA-021) and pho4(A) pho4(B) (PJA-026) cells. RNA concentration was assessed by Qubit assay (ThermoFisher) and its integrity with an Agilent 5400 Fragment Analyzer System. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, synthesis of the first strand cDNA was achieved by means of random hexamer primers, followed by the second strand cDNA synthesis using dTTP. After end repair, A-tailing, and adapter ligation, fragments were size selected, PCR amplified, and purified. Libraries were then pooled and subjected to sequencing in a NovaSeq X Plus equipment (Illumina) by Novogene Co., Ltd.