Genomic DNA was extracted from individual bacterial isolates, which were candidates to be part of new mycoplasma species infecting bovine. The same DNA extraction for each isolate was used for both Illumina and Nanopore library preparation. This hybrid sequencing strategy was used to generate complete and accurate bacterial genome assemblies by combining the high accuracy of Illumina short-reads with the long-read length of Nanopore sequencing to resolve repetitive elements and structural variants. Illumina libraries were prepared using a standard Illumina library prep kit and sequenced on an Illumina NovaSeq X, generating 2x150 bp paired-end reads. Nanopore libraries were prepared using the Oxford Nanopore Ligation Sequencing kit and sequenced on a PromethION instrument, generating long reads.