RNA was extracted using TRIZOL and dissolved in RNase-free H2O according to the reagent's instructions. 100 ng of total RNA per sample was used for library preparation. Ribosomal RNA was removed using the NEBNext rRNA Depletion Kit, and libraries were constructed with the NEBNext Ultra II Directional RNA Library Prep Kit. Library quality was assessed with the Bioanalyzer DNA-sensitivity kit.