We started from 10 sections 10 μm thick that were washed 3 times with xylenes to remove paraffin, rehydrated in an ethanol/water series. The tissue was resuspended in lysis buffer as previously described and sonicated for 5 minutes using a Covaris E220 instrument (setting: 140 peak incident power, 5% duty factor, and 200 cycles per burst) in 1 ml adaptive focused acoustics (AFA) fiber millitubes. Soluble chromatin (5 μg) was immunoprecipitated with 10 μg H3K27ac (Diagenode catalog number C15410196)) antibody (Ab). ChIP-seq libraries were constructed using ThruPLEX-FD kits (Rubicon Genomics) following the manufacturer's protocols. 75-bp single-end reads were sequenced on a Nextseq instrument (Illumina)