Nerve biopsies that had been snap-frozen were thawed on wet ice and homogenized using a Polytron Homogenizer PT3100 (Kinematica AG, Switzerland) in the presence of TRIzol Reagent. RNA extraction was performed according to the standard protocol provided by the manufacturer (Ambion, Thermo Fisher Scientific, MA, USA). Residual DNA contamination was removed using the DNA-free kit, following the manufacturer's instructions (Thermo Fisher Scientific Inc., MA, USA). The integrity of the isolated RNA was evaluated using 1% agarose gel electrophoresis and the TapeStation RNA ScreenTape system (Agilent Technologies, CA, USA). RNA sequencing libraries were constructed using the NEBNext Ultra™ RNA Library Prep Kit (New England Biolabs, MA, USA), with ribosomal RNA depletion via the NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, MA, USA).