After 24h of incubation cells were collected for single cell analysis. For each single cell run, 8 samples were combined. Each pre-incubated with a human specific barcode using Total Seq B antibodies. Vehicle and treated cells for each cell line were run in the same lane. Viability for each sample was above 80% Dissociated cells were processed for single-cell gene expression capture (scRNA-seq) using the 10X Chromium 3' Library, following the manufacturer's user guide