scRNA-seq of control and IL-1RAcP knockout across Candida infection timecourse
Mice were infected with Candida albicans as described in the main manuscript. At the indicated timepoints (naive, day 1, day 2 post-infection), tissues (tongue) were harvested, mechanically and enzymatically dissociated into single-cell suspensions, and filtered through a 40 µm cell strainer. Cell viability was assessed  prior to downstream processing. Single-cell suspensions were processed directly for library preparation. Cells were counted and viability confirmed (>85%) prior to loading onto the Chromium platform. Single-cell RNA-seq libraries were prepared using the 10x Genomics Chromium platform (Single Cell 3′ Gene Expression, v3/v3.1 chemistry) according to the manufacturer's instructions. Briefly, single cells were encapsulated into droplets with barcoded gel beads to generate Gel Bead-in-Emulsions (GEMs), followed by reverse transcription, cDNA amplification, and library construction.