Nuclei were isolated from antennae, stained with Hoechst and FACS sorted to separate them from debris. Then, they were processed using the CUT&RUN Assay Kit (Cell Signaling Technology, Cat#86652). Antibodies were purchased from Cell Signaling Technology (Cat#9751, 9733, 8173, 66362). Libraries were constructed using the manufacturer's protocol for NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs Cat#E7645).