Liver immune cell-enriched cell suspension were isolated after D collagenase digestion of the perfused liver of intFXR KO or Ctrl-flox mice, then labelled using one different CMO per mice. CD45+ cells were sorted using Influx cell sorter (BD) prior to single-cell encapsulation.. Sorted cells were loaded onto the 10x Genomics Chromium platform for single-cell partitioning and barcoding. Reverse transcription and subsequent library preparation steps were performed according to the manufacturer's instructions. Samples were prepared for single cell RNA-Sequencing with the Chromium Next GEM Single cell 3' Reagent Kit (Dual Index) (10x genomics), according to the manufacturer's instructions. Cells from all mice were labelled using Cell Multiplexing Oligos then treated in multiplexing following 10X genomics protocols to construct library upon sequencing. Final sequencing to an average depth of 100k reds/cell was performed by BGI (Hong Kong).