For bulk RNA-seq analysis of human peripheral blood NK cells, we sorted NK cells from peripheral blood using the Human NK Cell Isolation Kit (Miltenyi Biotec) or flow cytometric sorting with a purity of more than 95%. Total intracellular RNA was extracted using TRIzol (Invitrogen). RNA libraries for RNA-seq were prepared using VAHTS® Universal V8 RNA-seq Library Prep Kit or Fast RNA-seq Lib Prep Kit V2 following manufacture's protocols.