Genomic DNA (1 g) was fragmented (~350 bp) via Covaris ultrasonication. Libraries were prepared using standard Illumina protocols (end repair, A-tailing, adapter ligation, PCR), with size selection (AATI analyzer) and Q-PCR quantification (>3 nM). Pooled libraries underwent PE150 sequencing on an Illumina HiSeq 6000 (Novogene, China).