Genomic DNA was quantified using Qubit dsDNA quantification broad range assay (Thermo Fisher Scientific). DNA size was determined using the Femto Pulse System (Agilent). A total of 2 libraries were prepared for each sample to be sequenced across 3 SMRT Cells. DNA was sheared using Covaris g-tubes (Covaris 520079) to achieve an average size of 18-22 kb.Sheared DNA was size-selected on the PippinHT instrument (Sage Science) using the 6-10 kb or the 15-20 kb High-Pass definition. The size selected DNA was used as input for Pacbio SMRTBell Prep Kit 3.0 for library preparation. DNA damage repair, A-tailing and adapter ligation were performed as per manufacturer's instructions. SMRTBell Adapter Index Plate 96A was used for barcoding each library. Adapter ligated DNA was nuclease treated following the manufacturer's guidelines and purified using 1X Pacbio SMRTBell Cleanup beads. Final libraries were eluted in 30 µL Pacbio elution buffer and quantified using the Qubit dsDNA quantification high-sensitivity assay (Thermo Fisher Scientific). Final library size was determined using the Agilent Femto Pulse.Sequencing primer annealing and polymerase binding were performed using the Revio Binding Kit 3.0. Libraries were loaded onto the PacBio Revio machine utilizing SMRTlink v13 for workflow setup and loaded at 325pM loading concentration with 30 hours of movie time.