For cultured cells in 96-well plate, 20ul TCL buffer containing 1% 2-mercaptoethanol was used to lyse cells and 5ul of that was submitted to RNA-seq. Library construction and sequencing and data processing were performed according to the standard Immunological Genome Project protocols (https://www.immgen.org/img/Protocols/ImmGenULI_RNAseq_methods.pdf).