Cells cultured in 60 mm dishes were washed with pre-warmed DPBS, and then dissociated using Accutase after a 3-minute incubation at 37°C. Following dissociation, cells were centrifuged and resuspended in DPBS for single-cell library construction. Library construction for single-cell RNA-sequencing was performed according to the 10X Chromium Next GEM Single Cell 3' Reagent Kits v3.1 instructions. For each sample, 10,000 cells were targeted, and 10 cycles of amplification were performed for cDNA and library preparation.