Frozen CAR-T products stored in the GMP laboratory were thawed and resuspended in PBS. Single cells were encapsulated in Gel-Beads-in emulsion (GEMs) using the 10X Controller with a Chromium Single Cell 5' ver.3 GEM Library and Gel Bead kit. The TCR library was amplified using Chromium single-cell V(D)J enrichment kit (human T cell) (10x Genomics). KAPA Library Quantification kit (Roche) was used to quantify libraries.