Briefly, 1,0002,000 FACS-sorted CD4+ T cells were lysed in 5 L of TCL buffer (Qiagen) containing 1% 2-mercaptoethanol. Smart-seq2 libraries were generated from isolated cells according to the published protocol and sequenced at the Broad Genomics Platform on an Illumina NextSeq500 instrument using a High Output v2 kit (2  38 bp reads).