Total RNA was isolated from 70-80 microdissected SGLT2-negative LTL-positive tubules per sample using Arcturus PicoPure frozen RNA isolation kit and additional gDNA digestion was performed via Arcticzymes Heat & Run gDNA removal kit. Quality and quantity of total RNA were determined by High Sensitive Tape Analysis, and cDNA library was prepared using SMARTer Stranded Total RNA-seq Kit v3-Pico Input Mammalian with 0.5 ng total RNA (DV200>30%).