Total RNA, was isolated from podocyte-derived-EVs using the Total Exosome RNA and Protein Isolation kit (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA), following the manufacturer's protocol. All RNA preparations were stored at −80 °C until further use. Libraries were prepared from 6 μL of total RNA per sample using the Small RNA-Seq Library Prep Kit (Lexogen GmbH, Austria), following a protocol optimised for low-input samples. Individual libraries were normalised to 4 nM, pooled, and sequenced on the NextSeq 550 platform (Illumina, USA) using 150-cycle paired-end reads.