Tumours were mechanically minced and digested in a digestion buffer containing Collagenase I and DNAse I, followed by passing through a 21G needle and filtering through a 70um filter for preparation of single cell suspensions. Single cells were FACS-sorted for Live, CD45- CD31- EpCAM- Pdpn+ Pdgfra+ tdTomato+/- cell populations, before being pooled for on-chip multiplexing (GEM-X OCM 3' Chip Kit v4 4-plex 2 chips PN-1000747) Single-cell encapsulation and library preparation was performed according to manufacturer's instructions (GEM-X Universal 3' Gene Expression v4 4-plex Reagent Kits). Barcodes: T1A, T4A = OB1; T1B, T4B = OB2; T2A_3A, T5A = OB3; T2B_3B, T5B = OB4.