Cells were counted and viability was assessed using the LUNA-FX7 (Logos Biosystems) with Acridine Orange/Propodium Iodide Stain. Up to 29,000 cells were then loaded for capture onto one lane of a 10x Genomics Chromium GEM-X Single Cell 5' Chip v3.  Capture, barcoding and llumina sequencing library preparation were performed according to manufacturer protocols using the 10x Genomics Chromium X platform 5' version 3 GEM-X chemistry (protocol CG000733, revA). From each sample, cDNA was used for construction of both gene expression and TCR (VDJ) libraries. cDNA and libraries were assessed for quality via Tapestation 4200 (Agilent) and Qubit Fluorometer (ThermoFisher) and quantified by KAPA qPCR. Libraries were sequenced on an Illumina NovaSeq X+ (10B 100 cycle) flow cell with a 28-10-10-90 (R1-I1-I2-R2) asymmetric read configuration, targeting 20,000 barcoded cells with an average sequencing depth of 50,000 reads per cell and 15,000 reads per cell for the gene expression and TCR libraries respectively. Sequencing was performed by the Jackson Laboratory Single-Cell Genomics Lab and University of Connecticut Center for Genome Innovation.