RNA was extracted using the miRNeasy micro kit (Qiagen) from resting Tconvs, resting nTregs and edited iTregs. The quality of the RNA was assessed using an RNA 6000 Pico Kit on the 2100 Bioanalyzer System (Agilent Technologies). All sequenced samples had an RNA integrity number (RIN) of >8. RNA-sequencing libraries were prepared using the Smart-seq HT kit (Takara; 634455) in combination with the Nextera XT DNA Library Preparation Kit (Illumina; FC-131-1096) following the manufacturer's instructions. Sequencing was performed on the NovaSeq6000 platform (Illumina).