Total RNA was isolated and purified from homogenized mouse liver tissue in TRIzol reagent (ThermoFisher) following supplied protocol.  Isolated RNA samples were further purified using RNeasy mini kit (Qiagen). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.