TRIzol was added to snap frozen whole gastrocnemius muscle and was homogenized using the Qiagen TissueLyser. Following homogenization, RNA was extracted using the Direct-zol RNA Kit (Zymo Research). 300ng of RNA was used for library construction. Libraries for RNA-seq were prepared using the Lexogen QuantSeq 3' mRNA-seq FWD Kit for Illuming sequencing according to manufacturer's protocols.