RNA was extracted from fresh frozen tumour samplesusing AllPrep DNA/RNA kit (74104, Qiagen) according to the manufacturer's instructions. rRNA-depleted libraries were prepared using 200 ng of total RNA. Total RNA libraries were sequenced using Illumina NovaSeq 6000 XP SP, 200 cycles, paired-end reads (2 x 150 bp) 30 million reads. RNA preparation and sequencing were performed by GENEWIZ Azenta.