Tumor and normal kidney samples were thawed after previously being     cryopreserved. Dead cells were immunomagnetically depleted using the Dead Cell     Removal Kit (Miltenyi), washed, and resuspended in PBS with 0.04% bovine serum     albumin at a concentration of 1,000 cells per µL. Approximately 17,000 cells were     loaded onto a 10X Genomics Chromium instrument (10x Genomics). Libraries were     prepared using the Chromium single-cell V(D)J enrichment kit. Following library     construction, quality control, and quantification, libraries were pooled and     sequenced