Both dead and viable cell populations were single-cell deposited into individual wells of a 96-well plate, having a PCR-compatible Proteinase K-based lysis buffer (10 mM Tris-HCl (pH 8.0), 50 mM KCl, 2.5 mM MgCl2, 0.45% NP-40, 0.45% Tween-20, Proteinase K (100 µg/mL)), followed by 55°C, 30 min incubation to disrupt both cell and nuclear membranes and then heat-inactivation of the Proteinase K at 95°C for 10 minutes. Polyadenylated (polyA) messenger RNA (mRNA and lncRNA) was captured using Dynabeads Oligo(dT)25 magnetic beads (Thermo Fisher Scientific, Cat. No. 61002) and transferred to a separate 96-well processing plate for transcriptome library preparation. Reverse transcription (RT), template switching, and secondary cDNA amplification of the isolated polyA-selected mRNA fractions were conducted using the SMART-Seq Stranded Kit (Takara Bio, Cat. No. 634444) in strict accordance with instructions.