Four days after infection, cells were lysed in a lysis buffer containing Tris-HCl pH7.5 (50mM, Applichem, Germany), NaCl (150mM, Merck, Darmstadt, Germany), MgCl2,(10mM, Invitrogen, Schwerte, Germany), NP-40 (1%, Merck) dissolved in UltraPure DNase/RNase-free DW (Invitrogen) and supplemented with NaPPi (10mM, Merck), NaF (20mM, Applichem, Germany), okadaic acid (10nM, LC laboratory, USA), Na3VO4 (2mM, Sigma-Aldrich), PIM, (12µl/ml, Sigma-Aldrich), PMSF (4µl/ml, Carl Roth, Germany), SUPERaseIn RNase Inhibitor (200U/ml, Invitrogen), TURBO DNaseI (25U/ml, Invitrogen). Total RNA was purified using the miRNeasy Micro kit (QIAGEN, Hilden, Germany), according to the manufacturer's instructions. RNA and library preparation integrity were verified with LabChip Gx Touch (Perkin Elmer). RNA amounts were normalized and 500ng-1µg of total RNA was used as input for SMARTer Stranded Total RNA Sample Prep Kit HI Mammalian (Takara Bio). Sequencing was performed on the NextSeq2000 platform (Illumina) using P3 flowcell with 72bp single-end setup.