After 72 hours, cells were lysed in Trizol (Thermo Fisher) and total RNA extracted according to the manufacturer's protocol. Total RNA-seq was performed on two biological replicates by first conducting rRNA depletion using Ribo Zero Gold (Illumina) followed by library construction using the KAPA stranded RNA Library Prep kit (Roche). RNA-seq libraries were multiplexed and sequenced on the Illumina NextSeq 500 platform using the stranded 1 x 75 bp protocol by the Australian Cancer Research Foundation (ACRF) Cancer Genomics Facility.