Identifying crossover locations in Arabidopsis thaliana h2a.w7 Col-0 × Ler F2 populations using genotyping-by-sequencing
Leaf tissue at rosette stage were collected from 5-week old plants. Plant tissue was immediately snap frozen in liquid nitrogen and stored at -80oC. Plant were grown in growth chambers under the following conditions: 60% humidity, long day conditions (16 hours light/ 8 hours dark) at 20oC. DNA was extracted using CTAB procedure as previously described (Kuo et al, 2022 Methods Mol Biol - PMID: 35461443) DNA library preparation was prepared as previously described (Lawrence et al, 2019 Current Biology - PMID: 31378616) – TruSeq DNA sample prep kit. Illumina sequencing