The  mRNA in the enriched sample was purified using the Oligo d(T) beads, and the enriched sample were  subjected to library construction with GenSeq Low Input RNA Library Prep Kit (GenSeq, Inc.)  according to the manufacturers instructions. Libraries were controlled for quality and quantified using the BioAnalyzer 2100 system (Agilent Technologies, Inc., USA). Libraries sequencing was performed on  sequencer with 150bp paired end reads.