RNA was extracted with the Qiagen RNeasy Micro Kit. RNA purity was checked with a Nanodrop and quality was checked with an Agilent Tapestation. RNA from 5 mice with RIN>8.3 were pooled to generate each sample. >6 ng of RNA was sent to Novogene AIT Genomics Singapore Pte Ltd for library construction and sequencing. Libraries were constructed by Novogene (HK) Company Ltd with the Clontech SMARTer Ultra Low Input mRNA library construction kit. Paired-end 150 bp sequencing was performed on an Illumina Novaseq 6000.