Enhancement of suppressive function of Ly49+ CD8+ T cells in allogeneic immunity by CD80/86-CD28 blockade
Total RNA was isolated from samples prepared from MNCs infiltrating in the allografts using RNeasy Plus Universal Mini Kit (QIAGEN).  The quality of total RNA was assessed using Agilent Bioanalyzer 2200 system (Agilent Technologies, Santa Clara, CA).  Sequencing libraries were generated using NEBNext Ultra™ RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) following the manufacturer's recommendations.