Genomic DNA was fragmented, end-repaired, phosphorylated, and poly-A tailed. Libraries were constructed using Illumina DNA Prep Kit, with 300400 bp insert size selection by AMPure XP beads, followed by PCR enrichment and purification. Library quality was checked by Agilent 2100 and qPCR, and sequenced on Illumina NovaSeq X Plus (PE150).