Total RNA was isolated using the miRNeasy Mini Kit (QIAGEN, Germany). RNA concentration was determined with a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, USA), and quality control was performed using the Experion automated electrophoresis system (Bio-Rad, USA). The RNA Quality Indicator (RQI) was at least 9 for all analyzed samples. Libraries for next-generation sequencing (NGS) were prepared using the Illumina Stranded mRNA Library Prep Kit (Illumina, USA). Sequencing was performed on a NextSeq 500 system (Illumina, USA).