Total RNA was isolated from the specified biological materials using TRIzol Reagent (Thermo Fisher, cat. no. 15596026) following the manufacturer's recommended protocol. Polyadenylated RNA enrichment was conducted the total RNA using VAHTS mRNA Capture Beads (Vazyme, China, cat. #N401), following the manufacturer's prescribed protocol. and then components of KCTM Digital Stranded mRNA Library Prep Kit (Wuhan Seqhealth Co., Ltd. China) following the manufacturer's instruction