Transcriptomics profile of myobundles' maturation level
sample preparation began with gel digestion of muscle constructs using Trizol (TR118 MRC), followed by phase separation with 1-Bromo-3-chloropropane (B9673). RNA extraction from muscle cells was then performed according to ReliaPrep™ RNA Miniprep Systems' protocol (Promega), which included DNase treatment. NEBNext UltraExpress RNA Library Prep Kit for Illumina (New England BioLabs Inc.) was employed with the NEBNext Multiplex Oligos for Illumina (New England BioLabs Inc.) and NEBNext® Poly(A) mRNA Magnetic Isolation Module for cDNA synthesis with the addition of barcode sequences.