PKC-dependent MYRF dysregulation links Aβ pathology to oligodendrocyte, myelin and cognitive alterations in Alzheimer's disease
Samples derive from MACS-isolated O4+ oligodendrocytes. RNA was isolated from oligodendrocytes using the RNA Micro Kit (Qiagen).  Libraries were prepared with the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (#E6420S, New England Biolabs) and NEBNext Multiplex Oligos for Illumina (Index Primers 1 12) (#E7335S, New England Biolabs), following the corresponding instruction manual (Version5.0_5/20). The protocol started with 10 ng total RNA when available (otherwise, entire sample). Full-length cDNA was generated by template switching, followed by cDNA amplification and cleanup; 1 μl amplified cDNA was evaluated on an Agilent High Sensitivity DNA chip (#5067-4626, Agilent Technologies). 