Total RNA was extracted from RNAprotect Animal Blood Tubes using the RNeasy Protect Animal Blood Kit (Cat. No. 73224; Qiagen) following the manufacturers protocol. Globin reduction and library preparation were performed using the KAPA RNA HyperPrep Kit with RiboErase (Human, Mouse, Rat) method (Roche Diagnostics, Switzerland). Messenger RNA (mRNA) was isolated using poly-T oligo-attached magnetic beads, followed by fragmentation, cDNA synthesis, end repair, A-tailing, adapter ligation, size selection, amplification, and purification. Library quality was confirmed using Qubit, qPCR, and Bioanalyzer assays