Total RNA was extracted from the cells using RNeasy mini plus kit (Qiagen) following the manufacturer's protocol. RNA quality and concentration were assessed using a NanoDrop spectrophotometer. Only samples with an RNA Integrity Number (RIN) > 8.0 were used for library construction. Sequencing libraries were generated using the Illumina TruSeq RNA Sample Preparation Kit and subjected to end repair, A-tailing, adapter ligation, PCR amplification, and purification.