Total RNA was isolated using TRIzol Reagent (Thermo Fisher, 15596026) following the manufacturer's instructions. RNA integrity (RIN ≥ 8) was confirmed with Agilent 2100 Bioanalyzer. Poly(A)-selected stranded mRNA libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, E7770) according to the vendor's protocol.