Total RNA was extracted from bacterial pellets harvested after 48 h of co-cultivation of Ralstonia solanacearum and a synthetic Pseudomonas community (SynCom-A, consisting of 8 isolates) in 1/10 NB medium at 30 C and 170 rpm. After DNase treatment, high-quality RNA was selected for library construction using the Illumina TruSeq RNA Sample Prep Kit with rRNA depletion via the Ribo-Zero rRNA Removal Kit. Libraries were size-selected (200300 bp), PCR-amplified (15 cycles), and sequenced on the Illumina NovaSeq 6000 platform (PE158). Clean reads were trimmed with Trimmomatic and mapped to reference genomes using Rockhopper for gene expression quantification and differential analysis.