EAE was induced in C57BL/6 mice and CD4+ CD44+ GITR- Tconv cells were isolated from the brain and spleen of mice at day 60 post-immunization. 18718 (brain) and 38000 (spleen) CD4+ T conv, were subjected to Chromium Controller (10X Genomics) for single cell libraries generation using Chromium GEM-X Single Cell 5' Reagent Kits v3 (10X Genomics).