Total RNA was extracted by Direct-zol RNA Purification miniprep Kit (Zymo Research, R2052). RNA integrity (RIN) was confirmed by Bioanalyzer and RNA samples with maximum RNA integrity (RIN = 10) were used for Nanopore library preparation. We constructed Nanopore libraries using the Oxford Nanopore Direct RNA Sequencing Ligation Kit (SQK-RNA002) according to manufacturers instructions. We sequenced libraries on an Oxford Nanopore PromethION using a R.9.4 Flow Cell (FLO-PRO002) according to manufacturers instructions.