RNA was extracted using an RNeasy Plus Miniprep Kit (Qiagen) according to manufacturers directions. RNA was quantified on a nanodrop to confirm quality prior to library creation. Bulk RNA libraries were generated using a Mercurius High Sensitivity BRB-seq Kit (Alithea Genomics) using ~200 ng of RNA per sample according to the manufacturers directions. All samples were pooled after reverse transcription and final libraries were quantified using a Qubit dsDNA HS kit and BioAnalyzer. Libraries were sequenced on a single lane of an Illumina NovaSeq X using paired end sequencing. Samples were aligned using STAR (v2.7.11b) according to the kit directions. Samples were further demultiplexed using BRB-seqTools (v1.6.1) into single-sample .fastq files.