Freshly harvested tissues were formalin-fixed paraffin-embedded (FFPE) into tissue blocks. 5μm sections were cut for spatial transcriptomics assay using Visium HD 10x Genomics, and as per manufacturor's protcol Spatial transcriptomic sequencing libraries were prepared using 10x Genomics Visium HD FFPE Spatial Gene Expression Slides & Reagents Kit. Sequencing was performed using Illumina NovaSeq X Plus platform