Total RNA was extracted using the Maxwell RSC simplyRNA Cells Kit (Promega) following manufacturer's instructions. RNA concentration was assessed by NanoDrop and RNA integrity was evaluated on an Agilent TapeStation 4150. All RNAs had RINe > 7 Sequencing libraries were prepared from 150 ng of total RNA using the SureSelect XT HS2 mRNA System (Agilent Technologies), which combines poly(A) enrichment with strand-specific library construction. Indexed libraries were pooled at equimolar concentrations and sequenced on an Illumina NextSeq 550 in a 2×75 bp paired-end configuration.