Cerebral organoids were digested into single cells using Cell Recovery Solution for Organoids (Yeasen, 41421ES60), and approximately 2.5 × 105 cells were collected. The dissociated cells were cross-linked with Enhancer buffer and then combined with activated ConA beads and incubated overnight with BMAL1 antibodies. Following incubation, the supernatant was removed, and the samples were incubated with a secondary antibody (Vazyme, Ab207) for 1 hour at room temperature with rotation. Subsequently, the supernatant was discarded, and the ConA beads were incubated with pA/G-Tnp Pro and TTBL, each for 1 hour at both room temperature and 37°C. After TTBL incubation, 2 mL of 10% SDS was added to each sample and incubated at 55°C for 10 min. The fragmented DNA in the supernatant was enriched using activated DNA Extract Beads Pro next-generation sequencing library was constructed according to the manufacturer's protocol (Vazyme, TD202).In brief, mix DNA Extract Beads Pro, 2×CAM Master Mix, index primers N506 and N702, and DNA sample to a total volume of 100 ?l. Run PCR with 15 cycles following standard thermal conditions. After amplification, purify the product using VAHTS DNA Clean Beads: bind beads to the PCR product, wash twice with 80% ethanol, air dry, elute with ddH?O, and recover 20 ?l of purified DNA for storage at ?30 to ?15°C.