Fresh prostate tissue was dissociated into single-cell suspensions for 10x Genomics single-cell RNA-seq library preparation. Single-cell RNA-seq libraries were prepared using the 10x Genomics Chromium Single Cell 3' Gene Expression platform by the sequencing provider. The CellRanger web summaries report chemistry as Single Cell 3' v3 (polyA); the provider report describes Chromium Single Cell 3' Gene Expression Dual Index libraries and Illumina NovaSeq PE150 sequencing.