A reference model for circulating hematopoietic stem cells with applications to diagnostics
1 ml of PB was drawn from each individual into lithium-heparin tubes and used for DNA production.  DNA extraction and purification was performed using the Qiagen DNeasy blood and tissue kit to ensure high yields of high-quality DNA.   DNA library construction was performed using our in-house CH molecular inversion probe panel (as detailed in Beizuner, T. et al. An improved molecular inversion probe based targeted sequencing approach for low variant allele frequency. NAR Genom Bioinform 4, 2022.)