Detailed protocols can be found here https://www.biorxiv.org/content/10.1101/2025.07.01.661217v1 and in subsequent publications. All scRNAseq procedures were done according to 10X genomic's V3 3' SOPs. Detailed protocols can be found here https://www.biorxiv.org/content/10.1101/2025.07.01.661217v1 and in subsequent publications. All scRNAseq procedures were done according to 10X genomic's V3 3' SOPs.