Total RNA was isolated from FACS-purified cell populations using RNA Stat60 reagent according to the manufacturer's instructions. RNA quantity and integrity were assessed prior to library preparation. Poly(A)-selected stranded mRNA libraries were prepared using the KAPA HyperPrep Stranded mRNA Library Preparation Kit following the manufacturer's protocol. Libraries were sequenced as single-end 100-bp reads on an Illumina NovaSeq 6000 platform.