Cells were extracted using our previously published protocol for microvascular isolation (PMID 37056377). In short, brains were digested into microvascular fragments, which were isolated using magnetic beads coupled to CD31 antibodies. These fragments were then washed and redigested into single cells, which were then loaded onto a 10x Genomics Chromium controller or Chromium iX. Libraries were contructed according to manufacturers' instructions, using the 10x Genomics NextGEM single cell gene expression kit, v3.2