Total RNA was extracted after the manually microdissection of neoplastic cells from tumor-representative FFPE  8–10 µm thick sections using the Maxwell® RSC RNA FFPE Kit (Cat: AS1440, Promega Corporation, Madultison, WI, USA) on a semi-automated Maxwell® RSC extractor (Promega) and following the manufacturer's instructions Libraries for RNAseq were performed using Illumina Stranded Total RNA Prep with Ribo-ZeroTM Plus (Cat: 20040525, Illumina, San Diego, CA, USA) according to the manufacturer's protocol. Final libraries with optimal quality and quantity criteria, assessed by D1000 Screen tape kit (Agilent Technologies) and Qubit® dsDNA High Sensitivity Assay Kit (Thermofisher) were run on NextSeq550 sequencer (Illumina)