DNA-seq of Halomonas sp. DSM7328 original strain and evolved population in low dissolved oxygen level
Evolved population and original strain of Halomonas sp. DSM7328 were collected after overnight cultivation. Cell numbers around 2 x 10^9 cells were used for extraction. DNA from Halomonas sp. DSM7328 samples was extracted using DNeasy Blood & Tissue Kit (Qiagen) according to the manufacturer's protocols with pre-treatment of enzymatic lysis buffer. The sequencing library was constructed from 300 ng genomic DNA sample using the Illumina DNA Prep kit with Unique Dual Indexes. The library pool concentration was estimated using the Qubit dsDNA HS assay (Life Technologies). The library pool size was confirmed using the Agilent TapeStation 4200 (Agilent HS D1000 assay).