Cells were counted and viability assessed using a Countess 3 (Invitrogen, Carlsbad,CA). Cell suspensions were loaded onto a 10x Genomics Chromium X (Pleasanton,CA) using the NextGEM 3 V3.1 Gel bead kit. Cell capture, GEM generation, cDNA amplification, and library preparation were performed according to manufacturer protocol. The resulting 3 gene expression libraries were assessed for concentration and fragment size using the DNA High Sensitivity Assay on a GX Touch (Revity, Waltham, MA). The libraries were pooled, assessed by qPCR using the KAPA Library Quantification Kit (Complete, Universal) (Kapa Biosystems, Woburn, MA), and sequenced on an Illumina NovaSeq 6000 using 100bp PE reads (Illumina, San Diego, CA). The resulting sequence data were demultiplexed using Illuminas bcl2fastq2 Conversion Software v2.20. Each sample was analyzed using 10x Genomics Cell Ranger v8.0.0. The reference for gene expression was refdata-gex-GRCh38-2024-A, available at https://www.10xgenomics.com/support/software/cell-ranger/downloads.