Total RNA was isolated from treated macrophages using TRIzol or QIAzol followed by purification with the RNeasy Mini Kit (Qiagen). RNA quality and integrity were assessed using spectrophotometry, Qubit RNA HS assay, and Agilent Bioanalyzer RNA Nano 6000. RNA-seq libraries were prepared from 100 ng of total RNA using the Illumina Stranded mRNA Library Prep Kit according to the manufacturer's instructions. Libraries underwent AMPure XP bead cleanup, and library size and concentration were validated using an Agilent TapeStation. Pooled libraries were sequenced on an Illumina NextSeq 2000 platform using a P3 100-cycle XLEAP cartridge to generate 101 bp single-end reads with dual index reads