The genomic DNA of J53_W61C2-2_HD10 (one of three biological replicates of the C2 transconjugant, serial passaged for 10 days under antibiotic-high conditions) was extracted using the TIANamp Bacterial DNA Kit (Tiangen). The total amount of DNA was detected by fluorescent dye (Quant-iT PicoGreen dsDNA Assay Kit), and the integrity of DNA was detected by 1% agarose gel electrophoresis. Illumina NovaSeq libraries (TruSeq DNA Sample Preparation Kit, Illumina, USA) with 400 bp inserts and Paired-end 2  150-bp reads were sequenced to 100  coverage.