mRNA was enriched from total RNA using mRNA Capture Beads and then fragmented by incubation at elevated temperature. Using the fragmented mRNA as a template, the first strand of cDNA was synthesized in a reverse transcription reaction system. The second cDNA strand was synthesized simultaneously with end repair and poly(A) tailing. Adapters were then ligated to the resulting cDNA fragments. The ligated products were purified, and fragments of the desired size were selected using Hieff NGS® DNA Selection Beads. Finally, the libraries were amplified via PCR to enrich for adapter-ligated fragments.