Total RNA was purified from biopsies using QIAGEN AllPrep DNA/RNA Micro kit. Nucleic acids integrity was assessed with TapeStation (Agilent technologies 4200) and 0.48ng-20ng were used to prepare the library. RNA-seq libraries were prepared using the "Low_Input_mRNA_Novaseq6000_Gb" protocol and sequenced as 100 nt single-end reads on the NovaSeq 6000 platform (Illumina)