5E5 cells from bone marrow and spleen were lysed using Viagen DNA lysis buffer following manufacturers instructions. DNA Barcode-sequencing libraries were constructed using an amplicon approach. Briefly, viagen lysed samples were split in half to generate PCR replicates. Barcode sequences were amplified using primers flanking the constant region of the barcode before adding i5 and i7 indexes compatible with NGS sequencing. Samples were sequenced on an Illumina NextSeq2000 using 100-bp single end chemistry targeting 2 million reads per sample.