Total RNA was extracted from ARPE-19 cells transduced with Ad-miR-24. Poly-A mRNA was captured using oligo-dT priming for 3' end counting library preparation, with sample barcode and UMI incorporated during first strand synthesis. Second strand synthesis and tagmentation were performed to generate double-stranded cDNA fragments. Libraries were amplified using unique dual indices and sequenced on an Illumina platform to generate paired-end reads, with Read 1 containing barcode and UMI sequences and Read 2 containing transcript sequence information for gene expression quantification.