Mouse bone marrow was flushed with 2% BSA + 1X HBSS on ice to remove bone marrow. Bone marrow was filtered through a 70-micron screen, rinsed, then stained with fluorescent antibodies for lineage markers (CD2, CD3, CD8, B220, Gr-1, Ter119), c-Kit, Sca-1, CD150, CD48, CD45.1, and CD45.2. Cells were enriched for c-Kit using the Miltenyi AutoMACS system. Whole bone marrow (WBM) and Lineage-c-Kit+Sca-1+ (LSK) populations from CD45.1 or CD45.2 populations were sorted into separate tubes using a BD FACSAria II, counted, then recombined at a 3:1 (LSK:WBM) ratio. Cells were then centrifuged and resuspended in 0.04% BSA. Library was prepared using the Chromium Next GEM Single Cell 3' Kit v3.1 according to the manufacturer's instructions.