OT-I Cas9 knockin T cells where transduced with a lentiviral vector containing a non-targeting guide or a sgRNA targeting Elovl1. Transduced cells were selected based on CD90.1 expression and injected tail vein into recipient mice bearing hortotopic KPC_OVA tumors (ACT). The day after, the treatment with aPD-1 was started. 7 days post adoptive T cells transfer (ACT), mice were sacrificed and CD90.1+ OT-I T cells were sorted from the primary tumor. Cells from different mice were kept separetely and were considered as biological replicates. SMARTer cDNA library preparation