Whole lungs were chopped and homogenized in cold homogenization buffer (10 mM Tris, 5mM EDTA, 1% NP-40). An aliquot of the homogenate was mixed with 10 times the volume of Trizol. RNA extraction was done according to the ImmGen Protocol (https://www.immgen.org) “RNA extraction with Trizol”. Watchmaker Genomics mRNA kit