IL-18 Inhibition Aggravates Atherosclerosis in Jak2V617F Clonal Hematopoiesis
Ldlr-/- mice received 20% Jak2VF BMT and received anti-IL-18 or IgG treatment for 16 weeks. After 16 weeks, the aortas were then carefully isolated and subjected to enzymatic digestion. Cells were stained and subject to flow cytometry sorting. Sorted live CD45+ cells were submitted to scRNA-seq sequencing. RNA was extracted using the 10x Genomics Chromium Single Cell 3' protocol. The library was constructed using the 10x Genomics Chromium Single Cell 3' protocol.