High-molecular weight (HMW) genomic DNA of COLO320DM, COLO320HSR, PC3DM and SNU16 cells was extracted from approximately 2 million using MagAttract HMW DNA kit (Qiagen, 67563). After extracting HMW gDNA, we constructed Nanopore libraries using the Oxford Nanopore Ligation Sequencing DNA kit V14 (SQK-LSK114) according to manufacturers instructions. We sequenced libraries on an Oxford Nanopore PromethION using a 10.4.1. Flow Cell (FLO-PRO114M) according to manufacturers instructions. Basecalls from raw POD5 files were computed using Dorado (v.0.7.2). DNA reads were aligned to hg38 using minimap2 (v2.26).