Brains were quickly removed and the left hippocampus was dissected. Total RNA was isolated and purified using the RNeasy micro kit (Qiagen) according to the manufacturer's protocol. The quality and integrity of total RNA was assessed using a Tapestation 2200 (Agilent Technologies). RNA was considered as intact with an RNA Integrity Number (RINe) > 7. High purity RNA was considered with a OD260/280=1.8-2.1 and OD260/230 > 1.5 values. After purification, . Sequencing was performed on a NovaSeq 6000 System (Illumina) using cycle paired-end sequencing. mRNA was used for library preparation using the TruSeq RNA Library Prep (Illumina) according to the manufacturer's protocol