Expanded antigen-reactive T cells were sorted using live/dead dye, T cells markers, and tetramers and resuspended in PBS before library preparation. Expanded, reactive antigen-specific T cells were processed using 10x Genomics Single Cell 5' Gene Expression and V(D)J profiling kit and sequenced using Illumina NovaSeq 6000 at Advanced Technology Genomics Core of UT MD Anderson Cancer Center.