IRF4 CUT&RUN sequencing of murine alveolar macrophages after IL-33 treatment
Alveolar macrophages were collected by bronchoalveolar lavage (BAL). BAL was lightly fixed with formaldehyde (final concentration of 0.1%) quenched with glycine and single-cell suspension was stained with monoclonal antibodies followed by cell sorting. Additional information can be found in original publication. CUT and RUN was carried out with minor adaptations from the protocol  described in the following reference:  \"Skene, P. J., Henikoff, J. G. & Henikoff, S. Targeted in situ genome-wide profiling with high efficiency for low cell numbers. Nat Protoc 13, 1006–1019 (2018)..\"  Additional information can be found in original publication. Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina according to the manufacturer's instructions. Cleanup of libraries was performed with AMPure XP beads (Beckman Coulter). 