RNA-seq of human Genoskin explants treated with topical VTAMA (tapinarof) against untreated control
Skin explants were transferred immediately into culture medium. Prior to experimental manipulation, explants were equilibrated for at least 1 h at 37 °C in a humidified atmosphere containing 5% CO₂. Explants were cultured in 12-well plates for up to 5 days in a proprietary, chemically defined, hydrocortisone- and serum-free medium supplemented with penicillin (100 µg/mL) and streptomycin (100 µg/mL). Cultures were maintained at 37 °C and 5% CO₂, and culture medium was replaced daily. Skin explants were treated topically once daily with either vehicle control (Laboratoire Gola, Galenicare SA) or VTAMA (tapinarof cream, 1%, Dermavant Sciences) throughout the culture period. Total RNA from Genoskin samples was isolated using the RNeasy Lipid Tissue Kit (Cat. 74804, Qiagen) according to the manufacturer's instructions.  Illumina TruSeq Stranded mRNA protocol (Directional, first strand)