A specific reagent kit was used to construct a paid end library. After the library passed the quality inspection, NovaSeq 6000 was used for high-throughput sequencing in PE150 sequencing mode. The main steps of library construction include: genomic DNA disruption using ultrasound, DNA fragment end repair, adding an 'A' base to the 4 'end of the DNA fragment, adding sequencing adapters, fragment selection, and PCR amplification.