Nuclei: Fresh frozen tissue were OCT embedded, and 10µm frozen sections were cut with cryostat. Nuclei were isolated using 10X Genomics nuclei isolation kit according to manufacturers recommended protocol. FFPE: Five 25um sections were collected and dissociated according to 10X Genomics demonstrated protocol CG000632 revD. Viable Cells:  Tissues where dissociated using Accutase and the GentleMACS Octo dissociator Fresh_droplet_PolyA - Chromium Next GEM Single-cell 5' Kit v2 kit, Nuclei_droplet_PolyA - Chromium Next GEM Single-cell 5' Kit v2 kit, FFPE_droplet_Probe - Chromium Fixed RNA Profiling Reagent Kit, Fixed_droplet_Probe - Chromium Fixed RNA Profiling Reagent Kit, Fixed_in-situ_PolyA -  Evercode WT Mini v2 kit