Sequencing libraries were generated using the SMRTbell prep kit (PacBio, USA). High-molecular-weight genomic DNA was sheared into fragments with an average size of 15-20 kb. DNA fragments were end-repaired and ligated with hairpin adapters at both ends to form SMRTbell libraries. Unligated fragments and small library products were removed using exonuclease treatment. The qualified SMRTbell libraries were then bound with sequencing polymerase and loaded onto an SMRT Cell for sequencing on a PacBio Sequel IIe or Revio platform. The sequencing strategy employed circular consensus sequencing (CCS), which generates HiFi reads as the final output.