Primary astrocytes were plated at 1x10^5 cells per well in 2 mL in 6 well plates. One day after plating 2 uL of Dexamethasone (100 nM) was added to each well of the 6-well plates. One day after Dexamethasone treatment astrocyte medium was added and incubated for 24 hours. Following incubation samples were lysed with the Qiagen RNeasy Mini Kit according to the manufacturer's protocol.