DNA was extracted from a single larva from a lab-reared colony (as described above) using the MagAttract HMW DNA extraction kit (Qiagen, Hilden, Germany), following manufacturers specifications. The DNA sample was stored at +4 C and sent to IGATech (Udine, Italy) for library preparation according to the Ligation Sequencing Kit SQK-LSK114 protocol (Oxford Nanopore Technologies Limited, Oxford, UK) and sequencing on a PromethION flow cell (FLO-PRO114)