A developmental cell atlas of the human thyroid gland (Visium)
Fresh developmental thyroid samples provided by Human Developmental Biology Resource (HDBR) 10 Âµm fresh frozen tissue cryosections were collected onto a Visium slide and the 10X Genomics Visium Spatial Gene Expression protocol was followed (User Guide CG000239 Rev F)  H&E stained Visium slides were imaged at 40x on a Hamamatsu Nanozoomer 2.0 HT slide scanner. The tissue was permeabilized for 30 mins, a pre-treatment which was optimised in a separate time course experiment (10X Genomics, User Guide CG000238 Rev E). According to the manufacture visium protocol 10X Genomics, User Guide CG000238 Rev E According to the manufacture visium protocol 10X Genomics, User Guide CG000239 Rev F