For PacBio sequencing, high-quality genomic DNA (main band > 30 kb) was randomly interrupted into 15-18 kb fragments using a Covaris g-TUBE (Woburn, Massachusetts, USA). SMRTbell libraries were constructed in accordance with the manufacturers instruction using the PacBio HiFi Express Template Prep Kit 2.0 (Pacific Biosciences, Menlo Park, CA, USA). High-fidelity (HiFi) reads with high accuracy were generated on the PacBio Sequel IIe platform for de novo assembly of the female A. parallens genome.