Qiagen RNeasy Mini Kit- Quant-iT RiboGreen RNA Assay Kit- SureSelect cDNA Module (Pre PCR), then purified suing AMPure XP beads- SureSelect XT Low Input Reagent Kit with indexes 1-96- hybridized to Agilent SureSelect Human All Exon v.4 probes- The captured libraries were sequenced on Illumina NovaSeq 6000 platform for 2  150 paired end reads with an 8nt read for indexes using Cycle Sequencing v3 reagents