The DNA was subjected to damage repair and end-repair reactions, followed by ligation of SMRTbell adapters and nuclease treatment to remove unligated or incompletely processed fragments. The resulting SMRTbell library was size selected for fragments > 5 kb on a 0.75% agarose gel using a BluePippin instrument (Sage Science). The final library concentration (20.3 ng/L) was determined with the Qubit fluorometric assay prior to sequencing. Sequencing was performed using the PacBio Sequel II platform (Pacific Biosciences) with a 30-hour movie time.