Total RNA was isolated from mouse bone marrowderived macrophages (BMDMs). Genomic DNA contamination was removed by DNase treatment. Polyadenylated mRNA was then purified using the TruSeq Stranded mRNA Library Prep Kit (Illumina, Reference Guide #1000000040498 v00), which employs oligo-dT magnetic beads for polyA selection. The purified RNA was fragmented and reverse-transcribed to generate cDNA. After second-strand synthesis, end repair, and adapter ligation, the libraries were amplified by PCR and size-selected to obtain an average insert size of approximately 200406 bp. Paired-end sequencing was performed on the Illumina platform.