Mechanic cell lysis was performed and chromatin was sheared by sonication to fragments of 200-500 bp. 1 % of the chromatin extract was treated with RNase A and proteinase K and crosslinks reversed. DNA was purified using the QIAquick PCR purification kit 250 (QIAGEN 28106). For strand-specific library preparation the Accel-NGS 1S Plus Library Kit was used accordingly to the manufacturer's instructions. 2 ng of DNA were used as starting material and libraries amplified in 14 cycles. For clean-up SPRIselect beads were used.