Cells were crosslinked with 0.1% formaldehyde, quenched with glycine, harvested, and nuclei were isolated using cold Nuclear Extraction Buffer; after pAG-Tn5 tagmentation, nuclei were lysed with SDS and DNA was neutralized for downstream library preparation. CUT&Tag libraries were generated by non-hot start PCR amplification of the reaction mixture using universal i5 and barcoded i7 primers together with CUTANA High Fidelity 2× PCR Master Mix from Epicypher. Amplified DNA was purified with 1.3× AMPure XP beads from Beckman Coulter.