Single colonies were grown in 5mL liquid TY at 30C for 1 day. Then, the PacBio Nanobind CBB kit was used to extract high-molecular weight DNA from 1mL of culture. The DNA was sent for PacBio hifi long-read sequencing  at the W. M. Keck center at the University of Illinois, where gDNAs were sheared with a Megaruptor 3 to an average fragment length of 10kb then converted to barcoded libraries with the SMRTBell Express Template Prep kit 3.0 and pooled in equimolar concentration.