After treatment, cells were harvested and bound to Concanavalin A-coated magnetic beads. The plasma membrane was permeabilized with Digitonin, followed by antibody binding with anti-H3K79me2. pA-Tn5 transposase was added to perform targeted tagmentation. The resulting DNA was enriched by PCR to prepare sequencing libraries. mRNA was enriched from total RNA using oligo(dT) magnetic beads. After fragmentation, first-strand cDNA was synthesized using random hexamer primers, followed by second-strand cDNA synthesis using dUTP. The cDNA fragments then underwent end repair, A-tailing, adapter ligation, USER enzyme digestion, PCR amplification, and purification. Libraries were quantified using Qubit and real-time PCR and size-checked with a Bioanalyzer.