Following total RNA extraction, RNA-seq was performed at the NYU Langone Medical Center Genome Technology Center (GTC).  Bulk RNA-seq was performed with a NovaSeq 6000 instrument (Illumina). The fastq files were analyzed using the NYU Langone Medical Center's BigPurple cluster. SMART-Seq® HT Kit consisting of a one-step cDNA synthesis and amplification, purification and validation of cDNA and library preparation.