HN30  were treated with vehicle for 72 h. Cells were harvested in TRI reagent, and total RNA was purified using the QIAGEN RNeasy Mini Kit according to the manufacturers instructions. RNA quality was assessed using an Agilent TapeStation at the Penn State Genomics Core Facility, and four biological replicates per treatment group were selected for sequencing. RNA-seq was performed by SeqCenter, LLC (Pittsburgh, PA). RNA samples were treated with RNase-free DNase (Invitrogen), and libraries were prepared using Illumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus and 10-bp unique dual indices (UDI). Sequencing was performed on a NovaSeq X Plus platform generating 150-bp paired-end reads.