The brain was rapidly dissected and flash frozen in dry ice-cooled isopentane. The left prelimbic cortex was then dissected inside a cryostat at -20 degrees Celsius and embedded into a tissue array with sections from multiple replicates, balanced across sex and group. Tissue blocks were sectioned in a cryostat and positioned onto a Curio Trekker tile. UV treatment released the photo-cleavable spatial barcodes from the tile, allowing them to diffuse into the nuclei. Nuclei were purified and underwent 10x Chromium Single Cell 3'  (v3.1- Dual Index) with Feature Barcoding following the manufacturer's instructions. For samples with high-nuclei yields (Slides 1 and 2), we split the nuclei into two equal aliquots and ran them in separate 10X Chromium lanes.