Sequencing of the STARR-seq transfection plasmid library
DNA sequences of candidate cis-regulatory elements (CREs) were synthesized using a commercial service (GenScript) and cloned individually into the STARR-seq vector via Gibson assembly. Endotoxin-free plasmids were purified with ZymoPURE II Plasmid Maxiprep kit (Zymo) and DNA precipitation with 3M Na-Acetate pH 5.2 was performed if necessary to obtain desired concentrations. The sequencing library was generated with the NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs)