DRAQ5+,GFP+ cells were FAC-sorted to enrich the samples for Kenyon cells. mtdTomato expression was not used for sorting. Given the prior knowledge that different classes of Kenyon cells separate well during the analysis of single-cell data, the brains of flies where the subtypes of different classes were labeled (e.g. γd, α′/β′ap, and α/βs) were pooled in different combinations before the dissociation to minimize batch effects. Following manufacturer's instructions