Total RNA was isolated from plant tissue using the BIOCUBE Plant RNA prep kit (Biocube System Inc., Korea) according to the manufacturers instructions. The quality of the extracted total RNA was measured using Bioanalyzer 2100 (Agilent, SantaClara, CA, USA). Total RNA with an RNA Integrity Number (RIN)value greater than or equal to 7 was used for library preparation. RNA sequencing libraries were prepared using the TruSeq Stranded Total RNA with Ribo-Zero Plant Prep Kit according to the manufacturers instructions (Illumina, SanDiego, CA, USA). A libraries with the irrespective indices was paired-ends equenced using the NovaSeq X system (Illumina).