We extracted high molecular weight DNA from whole heads (eyes removed) using Monarch HMW DNA Extraction Kits for Tissue (New England Biolabs), following the manufacturers protocol. We sheared DNA to 1520 kb using Covaris g-TUBEs, prepared libraries with the SMRTbell Prep Kit 3.0 (Pacific Biosciences), and performed size selection (>10 kb) using a BluePippin system (Sage Science). We sequenced libraries on two PacBio Revio SMRT Cells with 24-hour movie acquisition.