For mRNA sequence, RNA was extracted from Kupffer cells of AKP liver metastases before and after chemotherapy using Trizol (Invitrogen) and reverse transcribed into cDNA using the HiScript II Q RT SuperMix kit (Vazyme). For RNA seq, libraries were prepared according to the illumina standard instructions. For scRNA seq, libraries were prepared using the Chromium Single Cell 3' Reagent Kit v2. For spatial transcriptomics,  libraries were prepared using the Stereo-seq Transcriptomics Set for Chip-on-a-slide (STOmics).