The lamina propria derived immune cells isolated from each of the four time points were hybridized with a 16-probe barcode set (PN-1000497, Probe Set v1.0.1). Cell viability and concentrations were assessed using the Countess II Cell Counter with AOPI viability dye (Fisher Scientific, NC2019981) to ensure equal pooling. Barcode and adapter ligations were performed by the McDermott Center Next Generation Sequencing Core at UT Southwestern Medical Center. Gel Bead-in Emulsion (GEM) generation was conducted using the Chromium Next GEM Chip and Chromium X instrument. Libraries were sequenced on an Illumina NovaSeq X Plus system using a 25B flow cell.