Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood using a Histopaque gradient. For cell hashing, samples were labeled with unique TotalSeq-B hashing antibodies. Cells were loaded onto a Chromium Next GEM Chip Q for GEM generation using the Chromium X system. Libraries were constructed following the 10x Genomics fixed RNA profiling protocol. During pre-amplification, TotalSeq C-additive primer was spiked in to amplify reads containing the Read 2 Nextera sequence. SCITO-seq2; Single-cell fixed RNA, protein, and hashtag oligo (HTO) sequencing