Total RNA was isolated using QIAzol Lysis Reagent (79306, Qiagen) and RNeasy Mini kit (74106, Qiagen) according to the manufacturer's instructions. cDNA was synthesized using SuperScript III Reverse Transcriptase (18080-044, Invitrogen). RNA-Seq libraries were prepared using Illumina TruSeq Stranded mRNA Library Preparation Kit (20020595, Illumina) according to the manufacturer's protocol.