The genomic landscape of non-small cell lung carcinoma in never smokers: whole-exome sequencing
In accordance with the study protocol approved by institutional review boards of Universitair Ziekenhuis Brussel and Antwerp University Hospital, NSCLC patients with history of non-smoking (strictly never smoked or smoked less than 100 cigarettes in their life time) or former limited smoking (smoked less than five packed years with a smoking free interval of more than 20 years) were enrolled from 2013 to 2015 for the present study, which is called the Belgian FIELT-2 study. The majority of these patients had stage IV disease. Genomic DNA extraction from tumour and matched normal tissue were performed with the QIAamp DNA FFPE Tissue Kit and the QIAamp DNA Blood Midi/Maxi Kit (Qiagen Inc., Valencia, CA) respectively, according to the manufacturer's instructions. Quality and quantity of purified DNA was accessed by the NanoDrop (Fisher Thermo, Wilmington, DE)  Whole-genome DNA libraries were created with the Illumina TruSeq sample preparation kit V2 according to manufacturer's instructions. Exonic fragments were captured with the Nimblegen SeqCap EZ Exome v3 or Agilent Sureselect Human All Exon V6 kit.