Human microglia were isoloated by dounce homogenization of the mouse brain, followed by removal of myelin via Percoll gradient and removal of mouse cells using the Miltenyi Mouse Cell Depletion kit. Immediately after isolation, microglia were fixed using the Parse Evercode Cell Fixation v2 kit. Cell barcoding and library construction was performed in accordance with the Parse Evercode WT Mini v2 protocol.