RNA-seq of mouse sorted alveolar macrophages
Experiment 3+4: AMs were harvested from bronchoalveolar lavage fluid at indicated timpoints after house dust mite challenge or schistosoma mansoni injection and FACS sorted into RLTplus buffer, supplemented with 1% Î²mercaptoethalol. Qiagen RNeasy Plos Micro kit following manufacturer's instructions Illumina stranded total RNA library prep kit was used following manufacturer's instruction