Total RNA from 5000 cells was extracted using the Qiagen RNeasy Kit following the manufacturer's protocol. cDNA was transcribed with a RNA, SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Takara Bio), amplified by 13 to 14 cycles of PCR, and purified with AMPure XP beads (Beckman). cDNA was quantified using a High Sensitivity DNA kit on TapeStation 4200 (Agilent). Library preparation was performed using a Nextera XT DNA library preparation kit (Illumina) according to the manufacturer's protocol. Libraries were normalized with beads, pooled, and sequenced on an NovaSeq X Series 10B Reagent Kit (300 Cycle) (read length 150-8-8-150).