Endophytic fungal communities were isolated from healthy spinach (Spinacia oleracea L.) leaf and root tissues. Surface-sterilized tissues were pooled for genomic DNA extraction using the DNeasy PowerSoil Kit (Qiagen, USA). The fungal ITS region was amplified using ITS1F (CTTGGTCATTTAGAGGAAGTAA) and ITS-2043R (GCTGCGTTCTTCATCGATGC) primers and sequenced on the Illumina MiSeq platform at QBiogen Laboratory, Chennai, India.