The pS1301-mCherry-LORE plasmid constructed by Shanghai Zhishuo Biology was verified by Sanger sequencing. The target fragment (LORE gene) was inserted into the vector backbone. Plasmid DNA was extracted from pS1301-mCherry-LORE and subjected to Sanger sequencing for verification. Plasmid DNA was extracted from each colony and sequenced in a one-directional manner using specific primers to confirm the correct sequence and absence of unexpected mutations. The sequencing library was derived from synthetic plasmid DNA and was specifically used for verifying the validation of the cloned insert.