Nuclei were isolated from frozen human post-mortem brain punches by tissue homogenisation on ice in 400 µl of homogenisation buffer using a pre-chilled dounce homogenizer and pestle by gentle strokes until dissolved. The homogenate was filtered to remove debris greater than 30 µm in size and centrifuged for 5 min 400 g at 4°C. The supernatant was discarded and the pellet resuspended in 500 µl of staining buffer. 200 µl was then removed to use as an unstained control in FACS, and the remaining 300 µl was stained with 1 µl Hoechst stain. Nuclei were isolated by flow cytometery and resuspended in staining buffer/Nuclei Storage buffer (Sigma) for use in downstream applications. Cells were dispensed at a concentration of 30,000 cells/mL onto nanowells using the ICELL8 Single-Cell System (Takara). The cDNA libraries were generated using the SMART-Seq ICELL8 cx Application Kit protocol provided by the manufacturer. Sequencing was carried out on Illumina NovaSeq 6000 platform using 100 bp paired-end sequencing with dual indexing.