Peripheral blood mononuclear cells (PBMCs) were isolated from blood in the lavender tube via density gradient centrifugation using Lymphoprep (Serumwerk Bernburg AG, #1858,). Total RNA was extracted using the Takara RNA extraction kit (Takara, TKR-9109). Eukaryotic mRNA library was constructed and downstream sequencing was performed using the DNBSEQ platform (150 bp paired-end).