Trip6+/+ and Trip6-/- mice were initially used to establish an AOM/DSS-induced colon cancer model, then colon tumor were isolated and digested for single cell suspension. Single-cell RNA-sequencing (scRNA-seq) were performed by OE Biotech Co., Ltd. (Shanghai, China). Libraries were prepared with 10X Genomics Chromium Single cell 3 Reagent v3 Kits according to the manufacturers protocol.