At 48 h post IM injection of mRNA Cxcl10-LNP, mice were euthanized by carbon dioxide asphyxiation. Single cell suspension of cells were harvested from the inguinal lymph nodes, washed with PBS through a 70 m cell strainer. Samples were pooled per group and combined at equal proportion per individual sample.  Single cell suspensions were stained with 7-AAD (STEMCELL Technologies, BC, Canada), and viable cells sorted using BD FACSAria III Cell Sorter (BD Biosciences, NJ, USA). RNA was isolated using Chromium Next GEM Single Cell 5 Kit (10X Genomics) following the manufacturers protocol. Read coverage was calculated at 2 billion paired reads per 5 library samples.