Total RNA was extracted using QIAGEN RNeasy Plant mini kit and cleaned up by QIAGEN DNase on-column Kit. Libraries were prepared using the Illumina Stranded mRNA Prep, Ligation kit with IDT for Illumina RNA UD Indexes, following manufacturer's recommendations. Completed libraries were QC'd and quantified using a combination of Qubit dsDNA HS and Agilent 4200 TapeStation HS DNA1000 assays. The libraries were pooled in equimolar amounts and the pool quantified using the Invitrogen Collibri Quantification qPCR kit.