Genomic DNA was extracted using the Zymo Research Quick DNA Miniprep extraction kit. The sample was then fragmented using an enzymatic approach. Resulting DNA fragments were size selected (300-500bp) using AMPure XP beads, the fragments were end repaired and Illumina specific adapter sequences were ligated to each fragment. Each sample was individually indexed, and a second size selection step was performed (NEBNext Ultra II FS DNA Library Prep Kit for Illumina). Sample was then quantified, using a fluorometric method, diluted to a standard concentration (4nM) and then sequenced on PacBios Onso platform, using a Onso (300 cycle) kit, following a standard protocol as described by the manufacturer. 200 MB of 2x150bp paired-end reads were produced for each sample