Fourteen male gonorrhea patients (NG group) treated in the dermatological clinic from 3/2023 to 12/2024 were selected. Seventeen healthy individuals served as the control group (HC group). DNA was extracted from urethral swab samples, host sequences were removed, and the resulting data were subjected to high-throughput sequencing. The data were then analyzed to compare the differences in urethral microbial communities between the two groups.