RNA-seq of human beta-cell line EndoC-betaH1 with and without single nucleotide replacement in the human TIA1 gene (E384K mutation)
Single nucleotide replacement in the human TIA1 gene (E384K) in human EndoC-betaH1 cells was achieved by prime editing. Total RNA was isolated from five wildtype (WT 1-5) and three mutated (Mut 1-3) EndoC-betaH1 human beta-cell line. Total RNA was extracted by QIAGEN RNeasy Mini Kit, following manufacturer's instructions. Total RNA was subjected to rRNA depletion, fragmented, and converted into double-stranded cDNA. After end-repair, A-tailing, and adapter ligation, libraries were PCR-amplified and sequenced on an Illumina platform using paired-end reads.