RNA-seq samples were collected using TriZol to lyse cells, then extracted from cells usingQIAGEN RNA Extraction kit. Genomic DNA was removed using DNase I. TruSeq Stranded mRNA Library Prep kit (96 samples) was used to build mRNA libraries (Illumina # 20020595). Qubit DNA assay was used to determine DNA concentration. samples. Agilent 2100 Electrophoresis Bioanalyzer was used to check mRNA library size (~260bp for RNA libraries and ~150-900bp) and quality before the sequencing run. NextSeq 2000, along with P3 reagents (100 cycles) was used for the sequencing run (Illumina # 20040559). On average, >20M reads per sample were acquired, covering 75bp from both ends