TCR sequencing of pathogenic and non pathogenic Th1 and Th17 cells subsets
\"Th1 and Th17 cell subsets were sort-purified (FACSAria III, BD Biosciences, or Aurora CS, Cytek) from peripheral blood of HD and CF patients, or from lung-tissues of CF and non-CF patients, according to the expression of specific surface markers combination among CD4+IL-7R+CD25low cells:  CCR6-CXCR3+ (Th1 cells), CCR6+CXCR3-CCR5- (cTh17 cells), CCR6+CXCR3-CCR5+ (pTh17 cells), CCR6+CXCR3+CCR5- (Th1/17- cells), CCR6+CXCR3+CCR5+ (Th1/17+ cells).\"  Not applicable â€“ primary human cells ex-vivo sorted  No treatment  Total RNAs were extracted using the kit Quick-RNA MicroPrep (Zymo Research), the quality of RNAs was evaluated using the TapeStation 4200 (Agilent) and only RNAs showing a RIN> 7 were used for library preparation.   Sequencing libraries were prepared using the SMART Seq v4 PLUS kit (Takara) starting from 5ng totRNA per sample and sequenced with the NovaSeq6000 (Illumina) generating on average 57.6 million 100bp paired end reads (PE) per sample.  