DNA extraction from buccal mucosa was performed using the QIAmp 96 DNA QIAcube HT kit (QIAGEN), adhering to the manufacturer's instructions. The DNA libraries were prepared using Illumina DNA Prep with Exome 2.0 Enrichment (Illumina) and were sequenced on the Illumina NovaSeq 6000 RPT01P/Carlos Chagas Institute - Fiocruz Paran in the 2x100 bp configuration.