Cells from samples were individually tagged utilizing BioLegend TotalSeq-A mouse hashtags then pooled and loaded into the 10X Genomics Chromium Controller where cells were individually encapsulated in an oil droplet with a gel bead, forming Gel-Beads-in emulsion (GEM).  This was perfromed according to the Chromium Single Cell 3' GEM Library and Gel Bead kit protocol. 10X Genomics 3' v3 single-index library prepration protocol for 3' poly-A mRNA capture with sample multiplexing protocol adapted from Stoeckius et al.'s 2017 protocol, as seen: https://cite-seq.com/wp-content/uploads/2019/02/cell_hashing_protocol_190213.pdf scRNA with HTO sample multiplexing derived from (Stoeckius et al., 2017) protocol