HUES64 hESCs were nucleofected with ABE8e-V106W RNP (PCSK9 sgRNA) and harvested 16 h post-nucleofection. Total RNA quality was assessed by Agilent TapeStation (RIN > 7). Libraries were prepared from 200500 ng total RNA using rRNA depletion and a stranded (dUTP) RNA-seq library prep workflow (VAHTS Universal V10; Vazyme NR616-02). Libraries were sequenced on an Element Biosciences AVITI instrument (paired-end 150 bp).