Total RNA was isolated  using the Ambion Purelink Mini Kit according to the manufacturer's instructions and eluted in 30 µL of DEPC-treated H2O and stored at -80 °C. RNA sequencing libraries were constructed using NEBNext Ultra II RNA Library Prep by Illumina from total RNA (220-580 ng/sample) was then subjected to polyA-enriched mRNA sequencing using a Novaseq6000 sequencer (Illumina Corp) with the S4 flow cell and PE150 sequencing parameter