PA14 cultures were grown for 4h or 16h in TOC-normalized mucin degradation supernatants (60mL each), harvested by centrifugation, and snap-frozen. RNA was extracted using the RNeasy Mini Kit (Qiagen) with -mercaptoethanol lysis and DNaseI treatment, followed by cleanup with the RNA Clean and Concentrator kit (Zymo). rRNA depletion was performed using Ribo-Zero Plus [Illumina], and libraries were prepared using TruSeq RNA Library Prep kit (Illumina) per manufacturers instructions. Sequencing was performed on the Illumina NovaSeq6000 platform (50 bp paired-end reads) at the University of Minnesota Genomics Center.