By using a biopsy punch,  fresh-frozen hippocampal brain region from mice were placed into lysis buffer and dounced in pestles to extract the nuclei. Then, the nuclei were washed, incubated on ice, resuspended in buffer and blocked with albumin for being hased using biolegend antibodies. Samples were pooled and loaded onto Chromium single-cell V3 3'chip (10x Genomics) Nuclei were hashed and pooled and loaded based onto a Chromium single-cell V3 3'chip (10x Genomics) following manufater's protocol for GEM formation