Single cell suspensions were prepared via washing of IOs with cold Hank's balanced salt solution (HBSS, Gibco, Brooklyn, NY, USA), dissociation with TrypLE (Gibco, Brooklyn, NY, USA) and filtering through a 70-μm strainer. Dead cells and doublets were removed by use of the Dead cell removal kit (Miltenyi Biotec, Cologne, Germany) according to the manufacturer's protocol. Cells were fixed with Chromium Next GEM Single Cell Fixed RNA Sample Preparation Kit and hybridized with Chromium Next GEM Single Cell Fixed RNA Human Transcriptome Probe Kit according to the manufacturer's guidelines (10X Genomics, Pleasanton, CA, USA). After the hybridization, cell suspensions were washed several times, passed through 30 µm filter and single-cell gel beads in emulsion (GEMs) were created by use of the Chromium Next GEM Single Cell Fixed RNA Gel Bead Kit and Chromium X instrument (10x Genomics, Pleasanton, CA, USA ). Single cell libraries were constructed with Chromium Next GEM Single Cell Fixed RNA Hybridization & Library Kit (10x Genomics, Pleasanton, CA, USA ). Libraries were equimolarly pooled and were sequenced using Illumina NovaSeq X. single-cell RNAseq