RNA extraction (QIAgen RNeasy minikit, 74116) was performed using a QIAcube following the manufacturer's protocol. Nanopore's PCR-cDNA Barcoding Kit (SQK-PCB114.24) was used. The protocol selects for polyadenylated transcripts by ligating an adapter at the 3'OH end of the RNA molecule and performs strand switching at the 5' end. Samples were barcoded using the provided adapters through 14 to 16 PCR cycles and pooled together in batches of 16.