Day 11 post wound induction of 6 mouse ears were incubated epidermis side up in 2.5% Trypsin solution at 37°C for 90 min, and the epidermis was removed from the dermis. Single cell suspension was achieved by mincing, cutting, and running the sample through a 70 µm filter. Samples were then enriched for LCs by magnetic sorting following the standard protocol from the Epidermal Langerhans Cell MicroBead Kit (Miltenyi Biotec). Single-cell libraries were prepared using the 10x Genomics Chromium Single Cell 3' Reagent Kit (v3 chemistry) targeting polyadenylated transcripts, according to the manufacturer's protocol.