RNAseq of doxorubicin exposed SV40 immortalized human cardiomyocytes
After the exposure, cells were washed once with PBS and subsequently lysed in 0.5 mL QIAzol Lysis Reagent (79306, Qiagen). Total RNA was extracted using chloroform and isopropanol precipitation. 50 ng of total RNA isolated from SV40 immortalized cardiomyocytes samples were used as input for the NEXTFLEX® Combo-Seq™ mRNA/miRNA Kit (NOVA-5139-53, PerkinElmer). All RIN (RNA Integrity Number) values were 8 or higher. We performed 18 PCR cycles were performed during library preparation. RNA-Seq libraries were quantified on a Qubit 2.0 Fluorometer (ThermoFisher) and quality control checked on the 2200 TapeStation System (Agilent).