The Illumina DNA Prep Reagent Kit and epMotion 5075 automated handler were used for library preparation. The library was quantified with a Qubit 4.0 and diluted to 4 nM. After denaturation with NaOH and Tris-HCl, it was diluted to 20 pM. A 1.5 pM library was loaded onto the NextSeq 500 for paired-end 2x150 bp sequencing.