Total RNA was extracted using TRIzol reagent (Invitrogen) according to the manufacturer's protocol. RNA quantity and integrity were assessed using an Agilent 2100 Bioanalyzer, and only high-quality samples were used for downstream library preparation. . Sequencing libraries were constructed using a poly(A) enrichment protocol and sequenced on an Illumina NovaSeq 6000 platform to generate 150-bp paired-end reads.