The purified PCR products were used to construct a library using the NEXTFLEX@ Rapid DNA-Seq Kit (Bioo Scientific, Austin, Texas, USA): (1) linker ligation; (2) removal of self-ligated fragments using magnetic bead screening; (3) enrichment of library templates through PCR amplification; (4) recovery of PCR products using magnetic beads to obtain the final library. Sequencing was performed on the NextSeq2000PE300 platform from Ilumina (Shanghai Meiji Biomedical Technology Co., Ltd.).