Cells were isolated from whole blood of orthopedic surgery patients using the negative selection RosetteSep kit (STEMCELL Technologies). Cells were lysed in of TRI Reagent®. The RNA was isolated using the RNeasy mini kit (Qiagen). Libraries were constructed with the QuantSeq 3' mRNA-Seq Library Prep Kit FWD using 250 ng of RNA as input bulk RNAseq