BRBseq of human skin-derived precursor hepatic progenitor-like cells with varying polygenic risk score for hepatic fat content exposed in vitro to vehicle-matched control medium or triggers related to MASH
Samples were lysed directly in the cell culture vessels (Falcon 6-well Clear Flat Bottom Polystyrene) using Buffer RLT (QIAwave RNA Mini Kit, Cat no. 74534), supplemented with beta mercaptoethanol, and QIAshredder cell-lysate homogenizers (Cat no. 79656). Total RNA was extracted from adherent cells using the QIAwave RNA Mini Kit (Cat no. 74534) according to the manufacturer's protocols. The sequencing libraries were built by tagmentation using 50 ng of ds cDNA with the Illumina Nextera XT Kit (Cat no. FC-131-1024) following the manufacturerâ€™s recommendations.