Total RNA was extracted from three types of samples: tumor tissues from sgNTC/sgDnmt3a MC38-bearing C57BL/6 mice, MC38 cells with or without 5-Aza treatment, and CD8+ T cells with or without zymosterol treatment. RNA isolation was performed using the QIAwave RNA Mini Kit (Qiagen, Cat. No. 74536) following the manufacturer's standard operating procedure. Strand-specific RNA sequencing libraries were constructed with the TruSeq RNA Sample Preparation Kit (Illumina, Cat. No. RS-122-2001) according to the manufacturer's protocol. All libraries were sequenced on the Illumina NovaSeq 6000 platform by Genergy Biotechnology Corporation (Shanghai, China).