High-molecular-weight genomic DNA was extracted from the samples using the CTAB extraction method. DNA quality and concentration were assessed prior to library preparation USING ScanDrop2 and Qubit4. Sequencing libraries were prepared using the Oxford Nanopore Technologies Rapid Barcoding Kit 24 V14 (SQK-RBK114.24) according to the manufacturers instructions. Individual samples were barcoded and pooled for multiplex sequencing. Sequencing was carried out on an Oxford Nanopore Technologies MinION Mk1D device using R10.4.1 flow cells. Raw signal data were acquired using MinKNOW software and basecalled to generate FASTQ files using the standard Oxford Nanopore workflow. Demultiplexing and quality control of reads were performed using EPI2ME workflows. Genome assembly was conducted using the EPI2ME assembly pipeline with default parameters for long-read assembly, followed by consensus polishing to improve assembly accuracy.fastq