Total RNA was isolated by Trizol extraction Total RNA from HEK-293T cells in which the THUMPD1 gene has been knocked out (THUMPD1 KO) vs control (WT) were isolated by Trizol extraction and sequenced on NovaSeq 6000 S1 using Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus and paired-end sequencing.