Total RNA was isolated from each sample using the FreeZol Reagent Kit. The RNA quality and integrity were assessed using NanoDropTM One/OneC, Life Invitrogen Qubit® 3.0 fluorometer and Agilent 4200 TapeStation. 0.1-1 μg of total RNA per sample was prepared for library preparation. The NEBNext® Poly(A) mRNA Magnetic Isolation Module and NEBNext® UltraTM II mRNA Library Prep Kit for Illumina® were used for mRNA isolation and library construction following the manufacturers' protocols.