DNA methylation atlas of rat kidney under tubulointerstitial fibrosis
Kidney cortex (~30 mg) was dissected from 8-week-old male Sprague–Dawley rats under isoflurane anesthesia, immediately snap-frozen in liquid nitrogen, and stored at –80 °C until DNA extraction. Genomic DNA was extracted using the DNeasy Blood & Tissue Kit (Qiagen) following the manufacturer's instructions. DNA was quantified with Qubit dsDNA BR and checked for integrity (agarose gel ≥20 kb, 260/280 ≥1.8) DNA ( ~ 350 bp) was fragmented, end-repaired, A-tailed, and ligated to bisulfite-compatible adaptors, followed by bisulfite conversion. Sequencing libraries were constructed and quality-checked, then sequenced.