DNA was extracted and purified using the QIAGEN MinElute Reaction Cleanup Kit. For library preparation, fragmented DNA was amplified with Phusion High-Fidelity PCR Master Mix supplemented with HF Buffer (Thermo Fisher Scientific, catalog no. F531S) and indexed primers featuring unique dual barcodes. Libraries were purified with AMPure XP beads (Beckman Coulter, catalog no. A63881) at a 1:1 ratio, adhering to the manufacturer's guidelines. Library quality assessment was performed using the Agilent Bioanalyzer 2100 in combination with the DNA 7500 kit (catalog no. 5067-1504), and concentrations were determined through real-time PCR with the KAPA Library Quantification Kit for Illumina (KAPA Biosystems, catalog no. KK4835). Pooled libraries were sequenced in paired-end mode utilizing a P2 flow cell on the NextSeq 2000 platform, employing Illumina reagent kits as required.