Single cell suspension was collected from four huLangerin-CreER; Rosa-stop-tdTomato; Cx3cr1-GFP; K14-H2B-Cerulean mice during homeostasis. Ears were incubated epidermis side up in 2.5% Trypsin solution at 37°C for 90 min, and the epidermis was removed from the dermis. Single cell suspension was achieved by mincing, cutting, and running the sample through a 70 µm filter. LCs and epithelial cells were sorted using fluorescence-activated cell sorting (FACS) based on their red (LCs) and blue (epithelial cells) fluorescence. Libraries were prepared using the KAPA mRNA HyperPrep Kit with dUTP-based strand specificity. Libraries were normalized to 10 nM, then loaded onto an Illumina NovaSeq flow cell. The sequencing was performed using 100 bp paired-end sequencing on an Illumina HiSeq NovaSeq.