For each biological replicate, 10 heads were pooled in 1 mL of TRI Reagent and homogenized with RNAse-free plastic pestles. Total RNA extraction was outsourced to Novogene (Sacramento, CA, USA) and performed using standard procedures. Stranded mRNA sequencing libraries were prepared from purified poly-A-containing mRNA. Sequencing was performed on an Illumina NovaSeq X Plus.