the cell suspension was obtained through the sequence steps corresponding to the cell preparation guide (CG00053 Rev C, 10X Genomics) and adjusted to up to 5,000 target cells with viability > 95% By Chromium Next GEM Single Cell 3-Gen Expression v3.1 Dual Index (CG00315 Rev D, 10X Genomics) and Chromium Single Cell 3' Reagent Kit (PN1000268, 10X Ge-nomics), we generated GEM from those cell suspensions. Subsequently, with continu-ous procedures, including cDNA amplification and clean-up, we produced the scRNA-seq libraries.