Total RNA was extracted from samples using the RNAprep Pure Plant Plus Kit (DP441, Tiangen Biotech, Beijing, China) following the manufacturers protocol. RNA concentration and integrity were assessed using a Qubit 4.0 Fluorometer (Thermo Fisher Scientific) and Qsep400 Bioanalyzer (Bioptic Inc.), respectively.High-quality RNA samples were submitted to Wuhan Maiwei Metabolism Biotechnology Co., Ltd. for cDNA library construction.