Approximately 1x105 sorted cells were pelleted by centrifugation at 600 g for 5 minutes at 4C. After carefully removing the supernatant, the cells were gently resuspended in a Omni-ATAC transposition mix (50 ul 2x TD buffer, 5 ul TDE1 enzyme (Illumina), 33 ul PBS, 1 ul 1% digitonin, 1 ul 10% Tween-20, 10 ul water). Transposition reactions were incubated at 37 C for 60 min in a thermomixer with shaking at 1,000 r.p.m. Following transposition, reactions were diluted with FACS staining buffer and centrifuged at 800 g, 5 minutes, 4C on a swing bucket rotor. The supernatant was discarded, and cells were washed with 100 ul 1x diluted Nuclei buffer (10x Genomics) and centrifuged again at 800 g, 5 minutes, 4C on a fixed angle rotor. The supernatant was carefully removed and transposed cells were resuspended in 30 ul 1x diluted Nuclei buffer. 1 ul 1% SDS was added, and the mixture was incubated at 37C for 10 minutes and then used directly as input for ATAC-seq library preparation. Sequencing was performed on the NextSeq500 or NovaSeq platform (Illumina).