Microbial isolates were extracted using the D4300 ZymoBIOMICS DNA MiniPrep kit and the genomes were prepared using the PacBio SMRTbell PREP Kit 3.0 (PacBio). The high molecular weight DNA was mechanically sheared, repaired, converted into SMRTbell libraries, cleaned up, normalized, and pooled for loading onto the PacBio Sequel 2.