Total RNA was extracted from undifferentiated and differentiated MuSCs using TRIzol reagent. The quality of RNA samples was examined using the Agilent 2100 BioAnalyzer (Agilent, Santa Clara, CA, USA). The extracted RNA samples were used for construction of cDNA libraries. The cDNA library preparation and RNA-Seq analysis were performed by Theragen Etex (www.theragenetex.com, Korea). The libraries were prepared for 100-bp paired-end sequencing using the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara Bio, Kusatsu, Japan) according to manufacturer's instruction. The quality of the cDNA libraries was evaluated using the Agilent 2100 BioAnalyzer (Agilent). The cDNA libraries were quantified using the KAPA Library Quantification Kit (Kapa Biosystems, Wilmington, MA, USA) according to the manufacturer's instructions.