Cells were collected in TRIZOL. RNA was purified with the Zymo direct-zol 96-well RNA kit. 2 µl of 1:1000 diluted ERCC RNA Spike-In Mix 1 (ThermoFisher #4456740) was added to 100ng total RNA of each sample. This was used as input for library prep. The Watchmaker RNA library prep kit with Polaris Depletion - rRNA/Globin (HMR) was used for library prep