RNA-Seq of perirenal adipose tissue of Assaf suckling lambs whose dam were subjected to a dietary protein restriction at prepubertal age
Samples from perirenal fat perirenal adipose tissue were collected from each lamb after sacrifice at the commercial slaughterhouse. The tissue was preserved in an RNA-stabilization solution (Ambion RNAlater; Life Technologies) and stored at 4 °C for 24 h. After that, the RNA-stabilization solution was removed, and the samples were later frozen at -80 °C until RNA extraction. The RNA extraction was performed using the miRNeasy Mini KIT (Qiagen) modified to use in adipose tissue. Once the RNA was obtained, the cDNA library construction and sequencing were conducted at Novogene in Cambridge (UK) by using the UltraTM RNA Library Prep Kit (NEBNext®).