Total RNA was isolated from in vivo sorted Hpgds KO and Ctrl TAMs (Viable, CD45+, CD11b+, F4/80+ cells) using the RNeasy Minikit (Qiagen) following the manufacturer's protocol and resuspended in RNase-free water. Frozen samples were shipped to Novogene where the Plant and Animal Eukaryotic Strand Specific mRNA (WOBI) service was performed and sequenced paired-end (150bp) on a Novaseq X Plus instrument.