Total RNA was extracted from LP1 cells by lyses in TriFAST (Euroclone, EMR507100). DNA was removed with RNase-free DNase set (Qiagen, 79254) using RNeasy Mini Kit (Qiagen, 74104). RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina following manufacturer's instructions (NEB, Ipswich, MA, USA).