Fresh frozen cryosections (10µm thickness) of sagittal brain hemisections were cut and mounted on 10X Visium spatially bar coded slides. Tissue permeabilization, RNA extraction and second strand synthesis for bar coded cDNA library construction was performed according to the instructions in 10X Genomics Visium Kit PN-1000189 Spatial RNA-seq libraries were prepared according to the manufacturer's guidelines (Visium, 10x Genomics). Resulting libraries were pooled and sequenced on an MGI G400 sequencer, followed by de-multiplexing using the library index sequences.