After completion of behavioral testing, mice were sacrificed and medial prefrontal cortex (mPFC) tissue was collected using a 1.5-mm tissue punch from coronal brain sections (+2.0 to +1.5 mm from bregma). Total RNA was extracted using TRIzol reagent (Thermo Fisher Scientific) according to the manufacturer's instructions. Libraries were prepared using the TruSeq Stranded mRNA Library Preparation Kit (Illumina) according to the manufacturer's protocol. Sequencing was performed on an Illumina NovaSeq 6000 platform with paired-end 150 bp reads to achieve a coverage of approximately 6 Gb per sample.