Fusion gene KMT2A::SEPTIN6 in AML cell line KOPM-88
Cell line was taken from the stock of the cell lines bank (Leibniz Institute DSMZ вЂ“ German Collection of Microorganisms and Cell Cultures). Cell line was authenticated by DNA profiling and cytogenetics. Total RNA was extracted via miRNeasy Mini Kit (Qiagen, Hilden, Germany) including DNase digestion. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads using Novogene's NGS Stranded RNA Library Prep Set (PT044). After fragmentation, the first strand cDNA was synthesized using random hexamer primers. Then the second strand cDNA was synthesized using dUTP, instead of dTTP. The directional library was ready after end repair, A-tailing, adapter ligation, size selection, enzyme digestion, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and Agilent Fragment Analyzer for size distribution detection. Quantified libraries were pooled and sequenced on Illumina NovaSeq X Plus, according to effective library concentration and data amount.