All barcoded scRNA-seq libraries were prepared with previously described protocols, using Chromium Single Cell 3 Reagent Kits version 3.1 (10X Genomics, PN-1000121). Briefly, suspensions with approximately 2X106 single-cells per sample were used to generate a gel bead-in-emulsion. cDNA libraries were processed according to 10X Genomics protocols. Sequencing was performed on an Illumina NovaSeq6000 platform