Total RNA was extracted from sgNC-transfected HepG2 cells using TRIzol reagent. RNA integrity was assessed by Agilent Bioanalyzer. Poly(A)-tailed mRNA was enriched using oligo(dT) magnetic beads, fragmented, and reverse transcribed into cDNA. After end repair, 3' end adenylation, and adapter ligation, the cDNA libraries were PCR amplified and purified. Single-end sequencing was performed on the MGI platform.