Identifying GFAP-Expressing Cell Susceptibility to SARS-CoV-2 Infection using Human iPSC-derived neural cells
The iPSC-derived brain cells were trypsinized and dissociated using 0.5% trypsin in PBS, and then resuspended in 0.04% BSA in PBS. A 40-μm strainer was used to remove cell debris and clumps, to obtain single-cell suspension. Nucleic acids were extracted following the 10x Genomics Chromium Single Cell 5' Reagent Kits v2 User Guide, up to the cDNA synthesis step. After GEM (Gel Bead-in-Emulsion) generation and reverse transcription, emulsions were broken, and barcoded cDNA was purified using silane magnetic beads, according to the manufacturer's instructions, with a target recovery of 10,000 cells per sample. Single-cell transcriptomic libraries were generated following the Chromium Single-cell 5' Reagent Kits V2 User Guide.