Total RNA was isolated following cells lysis on dishes using Direct-zol RNA Microprep Kit (Zymo Research, #R2061), followed by cDNA synthesis with qScript cDNA Synthesis Kit (Quantabio, #95047-025). A quantity of 1 μg of total RNA was used for mRNA purification with the NEBNext poly(A) mRNA magnetic isolation module. NEBNext Ultra II directional RNA library preparation kits for Illumina were used for RNA-seq library preparation. NEBNext multiplex oligos for Illumina were used for indexing during PCR amplification of the final libraries.