Sequencing libraries were generated using NEB Next Ultra  DNA Library Prep Kit for llumina(NEB, USA) following manufacturers recommendations and index codes were added. The library quality was assessed on the Qubit 2.0 Fluorometer (Thermo Scientific) and Agilent Bioanalyzer 2100 system. At last,the library was sequenced on an llumina MiSeq platform and 250bp/300bp paired-end reads were generated.