RNA-seq of mouse sorted alveolar macrophages
Experiment 1:  WT or IRF4-/- alveolar macrophages (AMs) were culture expanded and transferred into depleted SPAM-deleter animals. After 1 week, all mice were subjected to IL-33 induced inflammation (3 consecutive doses of 100 ng intratracheally). AMs were harvested from bronchoalveolar lavage fluid 1 day after the final dose of IL-33 and FACS sorted into RLTplus buffer, supplemented with 1% Î²mercaptoethalol. Qiagen RNeasy Plos Micro kit following manufacturer's instructions Illumina stranded total RNA library prep kit was used following manufacturer's instruction