cDNA libraries were generated using the PCR cDNA Sequencing Kit SQK-DCS109 (Oxford Nanopore Technologies), starting with 100 ng of poly-A RNA. Libraries were loaded onto R9.4.1 PromethION flow cells mounted on a P2 Solo and run for 96 hours. Basecalling was performed using MinKNOW software with the high-accuracy model on a GridION sequencer (Oxford Nanopore Technologies).