Total RNA was extracted from fetal penile tissues using TRIzol Reagent (Invitrogen) according to the manufacturer's instructions. RNA concentration and purity were determined using a NanoDrop spectrophotometer, and RNA integrity was assessed using an Agilent 2100 Bioanalyzer. Total RNA was fragmented and subjected to methylated RNA immunoprecipitation using an anti-m6A antibody. Both input RNA and immunoprecipitated RNA were used for library preparation according to the manufacturer's instructions. Libraries were sequenced on an Illumina NovaSeq 6000 platform using paired-end sequencing.