OT-I Cas9 knockin T cells where transduced with a lentiviral vector containing a non-targeting guide or a sgRNA targeting Elovl1. 3 days post transduction, transduced cells were sorted based on CD90.1 expression. Total RNA was isolated using the RNeasy Minikit (Qiagen) following the manufacturer's protocol and resuspended in RNase-free water. Frozen RNA was shipped to Novogene for the Plant and Animal Eukaryotic Strand Specific mRNA (WOBI) service.