Total RNA was isolated from mouse livers using RNeasy Micro Kit from Qiagen (Hilden, Germany). RNA Quantity was determined using a Qubit (TM) fluorometric assay and quality was determined using Eukaryote Total RNA Pico (Agilent Bioanalyzer 2100) The library preparation and sequencing were done on a single lane (Illumina HiSeq4000) at 100bp pair-end (PE) reads and performed at the Vincent J. Coates Genomics Sequencing Laboratory at University of California (UC), Berkeley.