Gnathostoma spinigerum larvae (AL3) were collected from the livers of naturally infected eels (Monopterus albus) in Thailand. The species was confirmed by molecular analysis of individual G. spinigerum larvae using a conventional polymerase chain reaction (PCR) protocol previously published by Jongthawin.the libraries were prepared using the Ligation Sequencing Kit SQK-LSK110 (Oxford Nanopore Technologies) and the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing (New England Biolabs).