We conducted internal transcribed spacer (ITS1) fungal-specific1 gene sequencing assay. Briefly, the DNA underwent PCR, using forward (5'-ACACACCGCCCGTCGCTACT-3') and reverse primers (5'-TTTCGCTGCGTTCTTCATCG-3'). All primers were synthesized with Golay barcodes, providing unique dual barcodes for each PCR. Each barcoded primer pair was tested using negative control to ensure no contamination in the PCR reaction. PCR amplicons were pooled and purified using Agencourt AMPureXP beads (Beckman Coulter), and the library was prepared using the KAPA LTP library preparation kit (KAPA Biosystems). High-throughput sequencing of libraries was carried out on an Illumina MiSeq (Illumina, San Diego, CA) with a 2 300-bp paired-end read kit.