P1 retinae from 3 biological replicates each for control (Chd4 f/+Cre+) and mutant (Chd4 f/f Cre+) samples, for a total of 6 replicates were dissected, enzymatically dissociated using papain, and triturated in LO-OVO solution (1X LO-OVO (Bio Basic), 0.4% DNaseI in 1X PBS) following resuspension in 1X PBS. Approximately 20,000 pooled cells were used in a single Chromium™ run (3′ Library & Gel Bead Kit v2, PN-120237, 10X Genomics).