Culture of primary human and macaque neural progenitors were dissociated for 20 min in papain, spun down and resuspended in 0.5% BSA, 5% Trehalose in PBS and sorted with FACSAria Fusion cell sorter (BD) based on GFP (and mCherry) expression. Collected cells were resuspended in 0.02%BSA in PBS and captured following manufacture's protocol. Gene expression library was constructed according to manufacturer's instructions. sgRNA libraries were separately amplified from each cDNA library. To generate STICR barcode libraries, cDNA library was used as template following protocol described in Delgado et al. 2021, PMID: 34912114.