RNA extraction: The TRIzol method is used to extract RNA according to the manufacturer's instructions. RNA precipitates were washed with 75% ethanol and dissolved in RNase-free water.RNA quality control: RNA concentration is determined using NanoDrop. RNA integrity was measured using Agilent Bioanalyzer (RIN > 7.0). RNA libraries for RNA-seq were prepared using SMARTER mRNA-SeqLibrary Prep Kit following manufacturer's protocols.