RNAseq in quiescent and refed quiescent cells in Saccharomyces cerevisiae
Saccharomyces cerevisiae cells were inoculated at 0.1 OD/mL in rich medium (Yeast Peptone Dextrose 2%, YPD) and grown for 6-7 days into stationary phase in conditions favouring aeration. Then, 25 OD600 nm equivalent of cells were harvested, pelleted at 800g and flash-frozen then stored at -70ÂºC before processing. Total RNA was extracted using Ribopure Yeast RNA (invitrogen AM1926) Samples were processed at Novogene for polyA-enriched library construction