For CUT&Tag library preparation, approximately 100,000 of permeabilized nuclei were resuspended in 50 ul buffer Med1 (2 mM EDTA, 2% BSA, 300 mM NaCl, 20 mM pH=7.5 HEPES, 0.01% Digitontin, 0.5 mM spermidine, 0.01% NP40, 1x Protease Inhibitor Cocktail) containing 1 ul of the relevant antibody(H3K9me3: Abcam #176916; H3K27me3: Abcam #ab192985; H3K36me3: Abcam #ab9050; H3K4me3: Abcam #ab8580; H3K4me1: Abcam #ab8895; H3K27ac: Abcam #ab4729)and 1 ul pA-Tn5 transposase, and incubated with gentle rotation at 4 degrees overnight. The next day, excess antibody and pA-Tn5 were removed by three washes with buffer Med2 (2% BSA, 300 mM NaCl, 20 mM pH=7.5 HEPES, 0.01% Digitonin, 0.5 mM spermidine, 0.01% NP40, 1x Protease Inhibitor  Cocktail) and then added 10 mM MgCl2, incubated at 37 degrees for 1 h. Fragmented DNA was purified by Qiagen purification kit (Qiagen, #28106) and amplified using NEBNext High-Fidelity 2X PCR Master Mix (NEB, #M0541L).