For RNA-seq: Cell pellets were collected and sent to GENEWIZ, Inc. for total RNA extraction and library preparation. For CUT&Tag: Genomic DNA was extracted from cells using NovoNGS® CUT&Tag 4.0 High-Sensitivity Kit (Novoprotein, Cat. No. N259-YH01, China) following the manufacturer's instructions. RNA-seq libraries: Cell pellets were collected and sent to GENEWIZ, Inc. for total RNA extraction and library preparation. CUT&Tag libraries were constructed as described in the manuscript: cells were processed, followed by tagmentation, PCR amplification, and library purification using the NovoNGS® CUT&Tag 4.0 High-Sensitivity Kit (Novoprotein, Cat. No. N259-YH01, China) following the manufacturer's instructions.