Approximately 100 mg of each sample was stored in 1 mL RNAlater (Qiagen) for 7 days for stabilization. RNAlater was removed, and tissues were homogenized in 600 μL RLT buffer (Qiagen) in a 2 mL cryovial using a TissueLyser (Qiagen) and ceramic beads. RNA was isolated using the RNeasy MiniKit (Qiagen), according to the vendor's instructions. Residual genomic DNA was digested and RNA cleaned via the Zymo Clean and Concentrator-5 kit according to the manufacturer's instructions. Libraries were constructed by poly-A selection and sequenced via Illumina NextSeq 550 to a target depth of 50 million reads.