This RNA-seq experiment was designed to compare the transcriptomic profiles of immunologically cold B16-F10 melanoma tumors treated with non-invasive Alternating Electric Fields (AEFs) versus sham-treated controls. It includes three biological conditions: control, low-intensity AEFs (1-1.5 V/cm), and high-intensity AEFs (2-2.5 V/cm), each with three biological replicates. The goal is to identify AEF-induced genes and pathways, particularly those involved in activating the DAPK2-STING axis and CD8+ T cell-mediated anti-tumor immunity.