Once in destination they were checked for total RNA integrity using a Bioanalyzer RNA Nano 6000 chip. All the samples qualified to proceed with the library construction having an RNA Integrity Number (RIN)  9. NGS transcriptomic libraries were constructed using a TruSeq Stranded mRNA LT Sample Prep Kit (Illumina). To verify the size of PCR enriched fragments, the template size distribution was checked on an Agilent Technologies 2100 Bioanalyzer using a DNA 1000 chip. The sequencing of libraries was performed as paired-end 150 bp reads on an Illumina NextSeq platform.