shRNA transduced hTSCs were collected in cell pellets and frozen at -80C. Pellets were then thawed on ice, and DNA was extracted using the QIAamp DNA Mini Kit. Residual RNA was removed by treatment with 10 μg/mL RNAse at 37 °C for 5 minutes, followed by clean-up of genomic DNA with the QIAamp DNA Micro Kit. 200ng of purified DNA per sample was then subjected to bisulfite conversion with the EpiTect Bisulfite Kit, followed by secondary strand synthesis, tagmentation, and library amplification and purification using the Zymo-Seq WGBS Library Kit and the Illumina Nextera DNA Flex Library Prep Kit. Library size was analyzed by Agilent TapeStation D1000 ScreenTape Assay and quantified by qPCR using the KAPA Library Quantification Kit. Libraries were then sequenced on an Illumina MiSeq i100 with 300bp paired end reads at a depth of 500,000 to 1 million reads per sample.