Single-cell gene expression analysis of in vitro expanded CD4 T cells responding to HHV-6B and HCMV epitopes
Epitope-specific CD4 T cells were isolated from PBMC cultured in vitro in presence of epitope peptides (10 ug/mL) for ~12-15 days, with IL2 addition to the medium at days 4 and 8. Isolation was performed using DRB1*03:01 tetramers carrying the epitope peptides and flow cytometry-based cell sorting.  RNA from single cells were obtained using the 10X Genomics Chromium Next GEM Single Cell 5' Library & Gel Bead Kit v1.1, following manufacturer's instructions. Library was prepared using the 10X Genomics Chromium Single Cell 5' Library Construction Kit v1.1, following manufacturer's instructions.