Fecal samples were collected by placing each mouse into an empty, autoclaved, metabolic cage for 24 hours. Pellets were collected into sterile tubes and fresh frozen. Fecal DNA was extracted using the Stool DNA Isolation Kit following the manufacturers protocol. Fecal DNA was quantified using the Qubit 4 fluorimetric Assay. Libraries were prepared from 100 ng of total DNA using the Negedia Whole Genome Sequencing (WGS) service (Next Generation Diagnostics s.r.l.) which included library preparation with terminal end ligation, quality assessment and sequencing on a NovaSeq 6000 sequencing system using a paired-end, 2x150 cycle strategy (Illumina Inc.).