Genomic DNA was extracted using the KingFisher Apex instrument per manufacturer's instructions 100ng of gDNA was digested with TaqaI and MspI at 37⁰C. Following enzymatic digestion, samples were used for library generation using an RRBS Methyl-Seq System. In brief, digested DNA was randomly ligated and, following fragment end repair, bisulfite converted using a DNA Bisulfite conversion. After conversion and clean-up, samples were amplified using the RRBS Methyl-Seq System protocol for library amplification and purification.