Amplified yeast genomic DNA was eluted in distilled water (10 µg in 10 µl water). Amplified yeast genomic DNA was incubated with 8 µl of melphalan stock solution (1 mg per ml of DMSO) and 12 µl of DMSO at 37 °C for 1 hour. Melphalan-treated DNA was purified by using Phenol-Chloroform extraction and eluted in distilled water.                