Total genomic DNA was extracted from the samples and used for amplification of the bacterial 16S rRNA gene V3V4 region using primers ACTCCTACGGGAGGCAGCA and GGACTACHVGGGTWTCTAAT. Sequencing adapters were incorporated during library preparation. PCR amplicons were purified, quantified, and pooled to generate sequencing libraries. Library quality control was performed before paired-end sequencing on the Illumina NovaSeq 6000 platform.