The CUT&Tag assay was performed on fluorescence-activated cell-sorted HSCs (~10,000) from C57BL/6 mouse bone marrow using the NovoNGS® CUT&Tag 2.0 High-Sensitivity Kit (Novoprotein). The library constructed using NovoNGS® CUT&Tag 2.0 High-Sensitivity Kit (Novoprotein) for sequencing, according to the manufacturer's instruction