45 000 Muscle stem cells (ITGA7+/VCAM1+/CD11b-/CD45-/CD31-/Ly6a-) were isolated by Fluorescence Activated Cell Sorting (FACS) into 2%FBS in PBS for the Cut&Run on freshly isolated cells. For the Cut&Run of REST, cells were first  isolated by FACS and cultured in growth media for 1 week and then 100 000K cells were obtained to perform Cut&Run. Cut&Run of H3K27me3 on MuSCS and Cut&Run of REST  on myoblasts were performed using the commercially available Cut&Run Kit (Epicypher, 14-1048) according to the manufacturer's guidelines. Libraries were prepared using the Cut&Run Library Prep Kit (Epicypher, 14-1001) according to the manufacturer's guidelines.