After the samples passed the quality control, RNA was reverse transcribed into cDNA using the cDNA-PCR sequencing kit (Oxford Nanopore Technologies, SQK-PCS109) and the SuperScript IV First-Strand Synthesis System (Invitrogen, cat #18091050). PCR amplification was performed using LongAmp Taq 2X Master Mix (NEB, cat #M0287) with a predetermined number of cycles based on optimization. The obtained double-stranded cDNA was subjected to damage repair, end repair, and A-tailing. After magnetic bead purification, the cDNA library was generated using the SQK-LSK109 ligation kit (Oxford Nanopore Technologies, SQK-LSK109). The resulting library was quantitatively measured using a Qubit 3.0 Fluorometer (Invitrogen, USA). submitted_instrument_model: OXFORD_NANOPORE PromethION