Imaginal wing and eye-antennal discs were harvested from crawling third-instar larvae. Larvae were staged to within 116 and 119 hours after egg laying. For each replicate, ten discs were dissected into ice cold 1X PBS solution. Three biological replicates were dissected for each genotype. After dissection, discs were incubated in Trizol for 5 minutes to dissolve tissue, then frozen at -20ºC. RNA was isolated using standard Trizol RNA isolation procedure. Libraries were prepared using the Illumina TrueSeq RNA prep kit V2.