Genomic DNA was extracted from Hypoderma sinense larval tissue using a CTAB-based method following mechanical disruption and proteinase K digestion. A whole-genome shotgun library was prepared using a BGISEQ/MGISEQ-compatible workflow involving adapter ligation, PCR amplification, and library circularization. The library was sequenced on the DNBSEQ-T7 platform in paired-end 150-bp mode.