DNA extraction from host blood, nested amplificaton with 25 cycles Plas1 (5-GAG AAT TAT GGA GTG GAT GGT G-3) and HaemNR3 (5-ATA GAA AGA TAA GAA ATA CCA TTC-3) and 35 cycles with 3760F plus an overhang adapter (5-TCG TCG GCA GCG TCA GAT GTG TAT AAG AGA CAG-3) and HaemJR4 with another overhang adapter (5-GTC TCG TGG GCT CGG AGA TGT GTA TAA GAG ACA G-3). 386 samples per MiSeq run