Total RNA was isolated from MDA-MB-231 cells after siRNA transfection with either control (Scrambled), siNELFA, siYAP, or combined siNELFA+siYAP knockdown. rRNA-depleted, strand-specific libraries were prepared using the KAPA RNA HyperPrep Kit and sequenced as 150 bp paired-end reads on the Illumina NovaSeq X Plus platform