Seeds of RS31::RS31-GFP and 35S::GFP plants were sown on agar plates containing half strength Murashige and Skoog (MS) medium: 2.2 g/L MS (Duchefa M0222.0050), 0.5 g/L 2-morpholinoethanesulfonic acid (MES), 1% sucrose, and 1% agar. The plates were placed vertically in growth cabinets and incubated under 16 h/8 h light/dark cycle at 22 C and 60% humidity for 14 days. RS31::RS31-GFP and 35S::GFP seedlings were subjected to irradiation with 254 nm UV-light at a dose of 2000 mJ/cm2 (UVP CL-1000 UV crosslinker) on ice 4 h after lights on. iCLIP was performed essentially as described (Meyer et al., 2017; Kster & Staiger, 2021).