Genomic DNA from the clinical isolate Acinetobacter baumannii DUEML206_AB_66, obtained from a human surgical site infection wound swab in Barisal, Bangladesh, was extracted and prepared for whole genome sequencing. A paired-end library was constructed using standard protocols, and high-throughput sequencing was performed to generate raw reads for assembly and genomic analysis. The data will support studies of antibiotic resistance, virulence factors, and strain diversity in hospital-associated infections.