The scRNA-seq libraries were constructed using the Chromium Single-Cell 30 GEM (Gel Beads-in-Emulsion) Library & Gel Bead Kit v3, following the user manual's instructions (10xGenomics). The Single-Cell 30 Library protocol generated standard Illumina paired-end constructs. The libraries were quality-checked using the High Sensitivity DNA Assay Kit (Agilent Technologies, Santa Clara, CA, USA). RNA quantification was performed using the ABI StepOnePlus Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA), followed by high-throughput sequencing on the Illumina HiSeq2500.