High-fidelity (HiFi) circular consensus sequencing was performed to accurately resolve the precise junction of the chromosome I-II fusion in the Schizosaccharomyces pombe mutant strain LM573, which was constructed via telomere-mediated fusion following centromere weakening. Genomic DNA was extracted from log-phase yeast cells and sequenced on the PacBio Revio system to generate long reads for de novo assembly and structural variant analysis.