RNA sequencing was performed on Drosophila ovaries. About 10 pairs of ovaries from 2 to 7 days old females have been dissected per genotype, and total RNA was extracted by TRIZOL (Thermo Fisher) and the genomic DNA was removed by DNase I (Thermo Fisher). The RNA-seq library was made from polyA+ RNA using NEB Ultra II Directional RNA library prep kit (NEB). We used the Kapa LongRange DNA polymerase (Sigma) with TruSeq compatible primers to amplify the libraries.