Total RNA was extracted from CD14+ monocytes using the RNeasy Mini Kit (Qiagen) according to manufacturer's instructions. RNA quality and quantity were assessed using a NanoDrop spectrophotometer and Agilent Bioanalyzer. Only samples with RIN values ‚â• 7.0 were used for library preparation. RNA libraries were prepared using the TruSeq Stranded mRNA Library Prep Kit (Illumina) according to manufacturer's instructions.