Genomic DNA was extracted using the Monarch Genomic DNA Purification Kit for Blood and Tissue (New England Biolabs), following the manufacturers instructions. DNA quality was assessed using Qubit fluorometry. Approximately 1 g of purified DNA was used to construct the sequencing library with the Oxford Nanopore Technologies (ONT) ligation sequencing kit (SQK-LSK114), following the standard protocol. The library was loaded onto a PromethION R10.4.1 flow cell and sequenced on the ONT P2 Solo platform. Basecalling was performed using Guppy v6.5.7 with the fast basecalling model.