No extraction occurred prior to submission for single cell sequencing. Cells were in suspension in RPMI media supplemented with 10% FBS. Single cell capture and library preparation were carried out according to 10x Genomics Chromium Single Cell V3.1 NextGem platform. cDNA synthesis and amplification were performed according to the 10x Genomics protocols. Paired-end reads were generated with a length of 100 base pairs (PE100), and sequencing depth was optimized according to experimental requirements