Aortas were harvested from the aortic arch to renal bifurcation, cleaned, and pinned en face, and aortic intimal cells were isolated following gentle digestion with 0.15 W units/mL collagenase D (Sigma-Aldrich # 11088858001) and 167 units/mL DNAse I (Thermo Fisher Scientific # EN0521) in PBS-/- for 10 minutes at 37°C. Six male and female mice were pooled per sample. Samples were assessed for viability with Trypan Blue (Thermo Fisher Scientific # 15250061), prepared as described in the 10x Genomics Chromium Next GEM Single Cell 3' Reagent Kit v3.1 user guide, and sequenced paired-end using the 10x Genomics Chromium platform (10x Genomics, USA).