Purified mRNA was eluted from the Dynabeads using SuPerfectRI Total RNA Isolation Reagent according to the manufacturers instructions, including a deoxyribonuclease (DNase) digestion step. Agilent RNA 6000 Pico Kit (5067-1513, Agilent, USA) and Agilent 2101 bioanalyzer were used to evaluate the quality and concentration of the purified mRNA.