Sorted cells were diluted to a concentration of 1,000 cells/l. A total of 16,500 cells, resuspended in the reverse transcription solution from the Single Cell 3 reagent kit v3.1, were loaded into the 10x Chromium Next Gel Bead-In_Emulsion (GEM) chip G, aiming for a targeted cell recovery of 10,000 cells. After loading the GEM chip into the 10x Chromium Controller, reverse transcription PCR was immediately performed to generate cDNA. The subsequent cleanup and amplification of cDNA products were conducted according to the protocol provided by 10x Genomics. A total of 10 l of amplified cDNA products were fragmented and indexed using primers from the Single Cell 3 reagent kit v3.1. The final libraries were quantified by Qubit 2.0 Fluorometer (ThermoFisher) and a 2100 Bioanalyzer with High Sensitivity DNA kit (Agilent) for quality check. The libraries were sequenced in paired-end mode using a S2 flow cell on a NovaSeq 6000 sequencer (Illumina).