Cell-associated RNA was extracted from mononuclear cells isolated from the liver, lung, spleen and lymph nodes of BLT mice treated as above using the Qiagen QIAmp viral RNA Mini Kit. DNase-treated total RNA (100 ng input for the liver, lung, and spleen samples, 50 ng for the lymph node samples) was depleted of ribosomal RNA and library constructed using NEBNext Ultra II Directional RNA kit