5µm thick sections were cut under RNase free codtions and processed according to the GeoMx RNA FFPE workflow (MAN-10150-02) with 10min antigen retrival at 100°C and 15min Proteinase K (0.1µg/ml in PBS) at 37°C for RNA Target exposure. Spatial Transcriptomics libararys were prepared according to GeoMx DSP workflow (MAN-10153-02)