To investigate whether delivery of in vitro synthesized RNA circle EPIC can affect endogenous circRNAs expression, we established psoriasis mouse model via IMQ (imiquimod) treatment at D0 and D1, and delivered (i.v.) EPIC by LNP at D1.5. We then performed total RNA sequencing of mouse (C57BL/6) spleens at D2.5 after intravenous injection. Each sequencing sample contained RNA isolated from spleen of one mouse.