67 dpf zebrafish larvae pan-neuronally expressing NLS-CaMPARI2 were used to label neurons that were active during the presentation of moving optic flow stimuli. Brains were carefully dissected, and cell dissociation was performed using papain. CaMPARI-red cells were sorted by FACS. Droplet-based RNA sequencing was performed according to the manufacturers instructions using the 10x Genomics Chromium System (Chromium Next GEM Single Cell 3' Reagent Kits v3.1).