Shotgun DNA sequencing was performed using the Ion S5 system. DNA sequencing libraries were prepared using the KAPA EvoPlus kit with 100 ng of input DNA, 4 min of fragmentation, and 7 cycles of final amplification. Primers and Y-adapters were designed according to Forth and Hper. Final size selection to 700 bp was performed using 2% E-Gel SizeSelect II agarose gels. Library size distribution and molar concentration were determined using a TapeStation system with D5000 ScreenTapes. Four barcode-tagged samples were pooled equimolarly and diluted to 50 pM. Automated template preparation and chip loading were performed on an Ion Chef system using the Ion 520 & Ion 530 Kit-Chef, and sequencing was performed on an Ion 530 chip.