Comparative transcriptome analysis of gill tissues between PBS-injected control and nervous necrosis virus (NNV)-infected juvenile seven-band grouper at 5 days post-infection.
Total RNA was extracted from gill tissues and used for library construction with the TruSeq Stranded Total RNA LT Sample Prep Kit (Gold). Library preparation included ribosomal RNA depletion, RNA fragmentation, cDNA synthesis, adaptor ligation, PCR amplification, and size selection. The libraries were sequenced on an Illumina NovaSeq 6000 platform using a paired-end 2 �� 101 bp configuration.