The LORE in RJK-V4-hIgG1-Fc plasmid constructed by Qingke Biology was verified by Sanger sequencing. The target fragment (LORE gene) was inserted into the vector backbone. Plasmid DNA was extracted from each colony and sequenced in one direction using specific primers to confirm the correct sequence and absence of unexpected mutations. The sequencing library was composed of synthetic plasmid DNA and was specifically used for verifying the cloned insertion fragment.