Cell capture and cDNA synthesis Using single cell 5 'Library and Gel Bead Kit (10x Genomics, 1000006) and Chromium Single Cell A Chip Kit (10x Genomics, 120236), the cell suspension (300-600 living cells per microliter determined by Count Star) was loaded onto the Chromium single cell controller (10x Genomics) to generate single-cell gel beads in the emulsion according to the manufacturer's protocol. In short, single cells were suspended in PBS containing 0.04% BSA. Single cell RNA-Seq library preparation According to the manufacture's introduction, Single-cell RNA-seq libraries were constructed using Single Cell 5' Library and Gel Bead Kit, Single Cell V(D)J Enrichment Kit, Human T Cell(1000005) and Single Cell V(D)J Enrichment Kit, Human B Cell (1000016). The libraries were finally sequenced using an Illumina Novaseq6000 sequencer with a sequencing depth of at least 100,000 reads per cell with pair-end 150 bp (PE150) reading strategy (performed by CapitalBio Technology, Beijing).