The single-molecule real-time (SMRT) PacBio genome sequencing library was constructed in accordance with the standard protocol of Pacific Biosciences. After the samples pass the inspection, the genomic DNA is fragmented. The fragments are selected using BluePippin, and after terminal repair and adding A tail, the ends of the fragments are respectively connected with connectors to prepare DNA libraries. Finally, the library was sequenced on the PacBio Sequel II platform