Total RNA was extracted with Trizol reagent (Invitrogen), and quality was assessed by NanoDrop (Thermo Scientific). Libraries were prepared from 3 g RNA following Illumina standard protocols: poly-A mRNA enrichment, fragmentation, first- and second-strand cDNA synthesis, end repair, adapter ligation, size selection (400500 bp, AMPure XP), and PCR amplification. Library quality was verified on an Agilent 2100 Bioanalyzer, and sequencing was performed on the Illumina NovaSeq 6000 platform (Shanghai Personal Biotechnology Co., Ltd.).