All steps involved in the biotin-miRNA streptavidin pulldown affinity purification were performed per established protocol 24. At both timepoints, purified miRNA-bound products were obtained from the experimental and control cells. In parallel, at both timepoints, transcripts altered (up- and down-regulated) by miRNA mimics were isolated (i.e. input). Cytoplasmic extract and Streptavidin-Dynabeads (M-280, Invitrogen) were prepared, then incubated for 4 hours. Biotin-miR-mRNA complexes were collected in a magnetic stand. (PMID = 25240884) For high-throughput RNA-sequencing (RNA-seq), libraries were constructed with Poly(A) selection by GeneWiz© for both the 12h (pulldown and input) and 72h (pulldown and input) products. In the manuscript pulldown is referred to as bound targets, and input is treated as RNA-seq to find differentially expressed genes. (PMID = 25240884)