The isolated DNA was subsequently quantified using a Qubit 2.0 fluorometer (Invitrogen) with the dsDNA HS Assay Kit (Invitrogen). Full-length 16S rDNA gene sequencing was performed using PacBio HiFi technology. The complete bacterial 16S rDNA gene (~ 1.5 kb) was amplified using universal primers targeting the V1-V9 regions (27F: 5-AGAGTTTGATCMTGGCTCAG-3 and 1492R: 5-GGTTACCTTGTTACGACTT-3) and sequenced using the Kinnex 16S rRNA Kit (PacBio). Quality assessment was performed using fastp (DOI:10.1093/bioinformatics/bty560), and the Circular Consensus Sequences (CCS) routine was used to generate HiFi reads. Between 47,525 to 84,684 sequences were obtained per sample, with a total numer of 2.860.378 reads