RNA seq of tumor draining lymph node (TdLNs) from a wildtype (WT) or tenascin-C (TNC) knockout (KO) mouse each bearing 4NQO-carcinogen induced tongue tumors
4-NQO was administered to 8 weeks old WT and TNCKO mice, which had been backcrossed with C57BL/6J mice for over 10 generations. The compound was delivered through drinking water at a final concentration of 100 μg/ml for 16 weeks. Following this treatment, the mice received a single 2 Gy dose of photon irradiation under anesthesia . After irradiation, the animals were provided with regular water for 6 weeks prior to sacrifice. At the time of euthanasia, tongue and mandibular lymph nodes (Tumor draining Lymph Nodes (TdLNs) were harvested.  Total RNA was extracted from lymph node samples using the Qiagen RNeasy Mini Kit according to the manufacturer's instructions. RNA quality and concentration were assessed using a NanoDrop spectrophotometer and Agilent Bioanalyzer 2100. Only samples with RNA Integrity Number (RIN) ≥ 8 were used for library preparation and downstream RNA-seq analysis. RNA-seq libraries were prepared using NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) and NEBNext Ultra II Directional RNA Library Prep Kit Illumina kits.