The purified RNA was used to prepare sequencing libraries with the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA) following the manufacturer's protocol. Briefly, mRNA was purified from 1 μg of total RNA using oligo (dT)-coupled magnetic beads and then fragmented at an elevated temperature. The qualified libraries were sequenced on an Illumina NovaSeq platform, generating 150 bp paired-end reads at Genomics, BioSci & Tech Co. in New Taipei City, Taiwan.