Cells were trypsinized in 2mL TrypLE, quenched 4mL full medium, spin down in 15 mL conical tubes. Cell pellets were then resuspended in 1mL PBS and transferred to centrifuge tubes. Cells were pelleted again. "Dry" cell pellets were shipped on dry ice to Azenta Life Sciences for RNA isolation and sequencing. Library preparation by Azenta Life Sciences; https://www.genewiz.com/public/services/next-generation-sequencing/rna-seq