Fresh fibroblast cells (∼2 million) suspended in media were pelleted (300xg 5mins, 4°C), washed twice with 5 ml PBS with 2% BSA to remove media, and resuspended in a final volume of 1.5 ml. The sample tube was split into 2 aliquots (2 x 700µl) containing approximately 1million cells per tube. Nuclei were extracted from one of the aliquots using the standard 10X Genomics protocols, while the remaining aliquot with intact live cells was used for single cell FACS. Single cells and single nuclei were checked for viability with a Luna-FL fluorescent cell counter, cell counts (99% live), nuclei counts (96% dead) as expected. For ResolveOme library sequencing, 4nM pool of all libraries was prepared combining each sample (genome and transcriptome libraries) at a 19:1 ratio DNA: RNA fractions, rather than a 4:1 ratio DNA:cDNA suggested by standard protocols. Sequencing (2×150bp) was performed on the Novogene 6000 instrument S4 chip, with a loading concentration of 1.8pM.