iPSC clones derived from the fibroblast line were thawed in mTeSR1 media (Stem Cell Technologies), centrifuged at 800 RPM for 4 minutes and each plated in a well of a 6-well tissue culture treated plate (Corning), pre-coated with a 1% solution of Geltrex LDEV-Free hESC-qualified Reduced Growth Factor Basement Membrane Matrix (Gibco Life Technologies). iPSC lines were cultured at 37°C, 5% CO2 and 5% O2. The media was changed every two to three days. After one week, the cells were passed using Gentle Cell Dissociation Reagent (Stem Cell Technologies) following the manufacturer instructions. Each clone was plated on one 10cm diameter tissue culture dish (Falcon) pre-coated with Matrigel as above. Between 5 to 8 days later depending on the clone, cells were passed again following the same procedure, and each plated on four 10cm Matrigel pre-coated dishes. iPSCs were harvested between 7 to 9 days later depending on the clones. At the time of harvesting, iPSC colonies were dissociated using TrypLE Express (Gibco Life Technologies), resuspended in fresh mTeSR1 media and counted using a manual hemocytometer. The single cell suspensions were resuspended in Cryostor CS10 (Stem Cell Technologies) at a density of 3 million cells per mL and 2 mL were aliquoted per cryovial (6 million cells per vial).