Total RNA was extracted using the Maxwell RSC 48 Instrument with the Maxwell RSC miRNA Tissue Kit (Promega, Madison, WI, USA) according to the manufacturers instructions. Sequencing libraries were prepared from qualified RNA samples using the Illumina TruSeq Stranded mRNA protocol (Illumina, San Diego, CA, USA) according to the manufacturers instructions. Paired-end sequencing (150   bp) was performed on the Illumina NovaSeq 6000 platform.