After 48h of treatment with atheroma plaque homogenates with DMSO or with  GSK2033, cells were harvested, stored at -80°C and transfer to Active Motif for tagmentation, library preparation and sequencing. ATAC-seq analysis were performed by Active Motif company (Waterloo, Belgium) using 1x105 cells per sample.  Filtered reads were aligned to the GRCh38 reference genome. Open chromatin regions were identified with HOMER.