Total RNA was isolated from eight control and twenty AD ITG brain tissue samples using PureLink RNA Mini Kit (Invitrogen, #12183018A). rRNA depletion using QIAseq® FastSelect™ rRNA HMR kit (Qiagen) RNA sequencing libraries were constructed at Genewiz (South Plainfield, NJ, USA) with the NEBNext Ultra II RNA Library Preparation Kit for Illumina.