Total RNA was extracted using the PicoPure RNA extraction kit (Arcturus) mRNA was extracted from the total RNA using NEXTflex Poly(A) Beads (Hiss Diagnostics) following the manufacturer's instructions. 14 μl of the mRNA elute was transferred to a fresh 1.5 ml tube (Eppendorf). Next, cDNA was synthesized using the NEXTflex Rapid directional qRNA-Seq kit (Hiss Diagnostics)