A total amount of 2 microg DNA per sample was used as input material for the ONT library preparations. After the sample was qualified, size-select of long DNA fragments were performed using the BluePippin system . Next, the ends of DNA fragments were repaired and A-ligation reaction were conducted with NEBNext Ultra II End Repair/dA-tailing Kit. The adapter in the LSK116 kit was used for further ligation reaction and Qubit Fluorometer was used to quantify the size of Library fragments. submitted_instrument_model: OXFORD_NANOPORE PromethION