30 Midguts where dissected in ice-cold PBS, taking care to remove Malpighian tubules, hindgut and proventriculus and samples were frozen in -80°C. DNA was extracted from dissected midgut and methylated fragments were isolated with DpnI and DpnII digestion. Methylated fragments were then amplified with PCR and sonicated in order to generate libraries suitable for sequencing. Sequencing was performed using single end 86 bp reads with a using a Nextseq 550 at the Deep Sequencing Facility, BioQuant, Heidelberg University.