ATAC-sequencing of murine alveolar macrophages after IL-33 treatment
Alveolar macrophages were collected by bronchoalveolar lavage (BAL). Single-cell suspension was stained with monoclonal antibodies followed by cell sorting. Additional information can be found in original publication. ATAC-seq was carried out with minor adaptations from the protocol  described in the following reference:  \"Buenrostro, J. D., Wu, B., Chang, H. Y. & Greenleaf, W. J. ATAC-seq: A method for assaying chromatin accessibility genome-wide. Curr Protoc Mol Biol 2015, 21.29.1-21.29.9 (2015).\"  Additional information can be found in original publication. Transposed DNA was amplified using a NEBNext High Fidelity 2Ã— PCR Master Mix (New England Biolabs) with indexed primers. Libraries were purified using SPRIselect beads (Beckman Coulter) to remove primer dimers.