Modelling group 3 medulloblastoma post radiotherapy
Fresh tumor tissues were resected under a stereoscope at endpoint. PDX tumor tissues were mechanically and enzymatically dissociated using a collagenase-based dissociation method. Approximately 10,000â€“16,000 tissue dissociated viable cells were loaded per sample on the Chromium Controller for encapsulation into gel beads-in-emulsion (GEMS) for reverse transcription of barcoded RNA.  Dynabead clean-up, PCR amplification and SPRIselect bead clean-up were carried out using the Chromium Single Cell 3' Gel Bead kit. Indexed single-cell libraries were generated using the Chromium Single Cell 3' Library kit and the Chromium i7 Multiplex kit. 