Single-cell suspensions were prepared from mouse dorsal skin. T cells in the single-cell suspensions were enriched using the EasySep Mouse T Cell Isolation Kit (STEMCELL Technologies) and further purified by soring with FACSAria (BD Biosciences). Sorted live T cells were loaded onto a Chromium chip for encapsulation and barcoding. Libraries were constructed using the Single Cell 5' Kit (10X Genomics). The resulting libraries were sequenced using NovaSeq 6000 (Illumina).