Metagenomic sequencing of benthic diatom community from Benthos river collected in August 2022 from Hainan island, China. IMPORTANT: Raw multiplexed sequence data were demultiplexed and split by individual sample barcode (index) to generate separate, independent data files for each sample. Each row represents one unique sample with its own individual sequence files. Library preparation and paired-end sequencing were performed using Illumina platform.