A million cells were freshly harvested and subjected to chromatin cross-linking using disuccinimidyl glutarate (DSG) (Cat# 20593) and 37% formaldehyde. Chromatin digestion was carried out using an endonuclease enzyme, and the digestion efficiency was monitored, and quality controlled using a Bioanalyzer. With approximately 80% of the fragments falling within the 100-2500 bps range, the captured and ligated chromatin underwent reverse crosslinking and DNA purification. Subsequently, the libraries were constructed using the Dovetail™ Library Module for Illumina (Cat# 25004). The final libraries were sequenced with 150bp paired-end reads on the NovaSeq 6000 platform modified Hi-C, with using endonuclease cutting The Dovetail® Omni-C® Library Preparation Kit (Cat# 21005) was employed for sequencing library preparation.