Cells were detached with trypsin/EDTA. After addition of full medium and centrifugation, cells were resuspended in PBS with 0.04 % BSA and passed through a 30‑μm filter. The number of viable cells was determined using an automated cell counter. Libraries were constructed according to the manufacturer's instructions (single cell 3' v3 protocol, 10x Genomics). In short, 10 000 live cells were loaded onto a 10x Genomics microfluidic chip and encapsulated with barcoded oligo-dT-containing gel beads using 10x Genomics Chromium controller.