Zebrafish embryos at 72 hpf from the Control-MO and wbp11-MO groups were collected, with three biological replicates per group and 50 embryos per replicate. Samples were preserved in RNAlater (Servicebio) and stored at -80 °C prior to being shipped on dry ice to Shanghai Personal Biotechnology Co., Ltd. for library construction, transcriptome sequencing, and bioinformatics analysis. mRNA with polyA tails was enriched from total RNA using Oligo(dT) magnetic beads and fragmented to approximately 300 bp using an ion_x001E_based fragmentation method. Following library construction, PCR amplification was performed to enrich the library fragments, after which size selection was conducted to obtain libraries of approximately 450 bp. Library quality was assessed using an Agilent 2100 Bioanalyzer, and both total concentration and effective concentration of the libraries were determined. mRNA with polyA tails was enriched from total RNA using Oligo(dT) magnetic beads and fragmented to approximately 300 bp using an ion_x001E_based fragmentation method. Following library construction, PCR amplification was performed to enrich the library fragments, after which size selection was conducted to obtain libraries of approximately 450 bp.