A shared dissociation protocol was used to generate live cell populations from fresh or cryopreserved foetal specimens. Four foetal samples were processed fresh immediately following dissection upon receipt (CAM00005, CAM00006, CAM00007, CAM00008), while all other foetal samples for single cell RNA sequencing were processed as cryopreserved samples. One early (CAM00018) whole head embryo sample was split between single nuclei RNA sequencing and single cell multiome. Samples were separately minced in dissociation media of 5ml of 0.1mg/ml Liberase TH, 1:1000 DNase I and gently rotated for 30 minutes. Dissociation was halted with a 5% FBS stop solution prior to resuspension in 1X RBC lysis buffer and viability assessment using a Countess. DFO5 R1 and R2 fastq files were split into two equally sized gzipped-fastq files using SeqKits split2 function with the -p 2 argument.