One hundred cells were directly sorted into lysis buffer. We performed RNA sequence as previously reported (Hayashi et al., 2018) with minor modification. Briefly, using one hundred sorted cells, the first strand of cDNA was synthesized by using PrimeScript RT reagent Kit (TAKARA Bio Inc., Kusatsu, Japan) and not-so random primers. Following the synthesis of the 1st strand, the 2nd strand was synthesized by using Klenow Fragment (3'-5' exo-) (New England Biolabs Inc., Ipswich, MA) and complement chains of not-so random primers. Using purified double-strand cDNA, the library for RNA-Seq was prepared and amplified using Nextera XT DNA sample Prep Kit (Illumina Inc., San Diego, CA).