Commercial pasteurized bovine milk samples were purchased in Kagoshima Prefecture, Japan, between November 27 and December 19, 2025. Three matched sample pairs were analyzed, consisting of milk derived from the same processing tank or source batch and subjected to either low-temperature long-time pasteurization (LT; 65 degrees C for 30 min) or ultra-high-temperature short-time pasteurization (UHT; 135 degrees C for 2 s). Extracellular vesicle-enriched fractions were isolated from milk by serial differential centrifugation. Briefly, milk was centrifuged at 5000 x g for 30 min at 4 degrees C to remove debris and fat. The intermediate aqueous fraction was then sequentially centrifuged at 10000 x g for 1 h, 35000 x g for 1 h, 70000 x g for 1 h, and 100000 x g for 1 h at 4 degrees C. The final pellet was resuspended in phosphate-buffered saline. RNA was extracted immediately from the extracellular vesicle-enriched fractions using the Invitrogen Total Exosome RNA and Protein Isolation Kit. Small RNA libraries were prepared using the QIAseq miRNA Library Kit and sequenced by GENEWIZ from Azenta Life Sciences on an Illumina NovaSeq 6000 system. Downstream preprocessing may require removal of reads containing the putative T7 RNA polymerase promoter-derived artifact core sequence TAATACGACGTACTTAG, allowing up to one mismatch.