RNAseq of a unique human heart 5 days post-myocardial infarction versus healthy donor controls
Premortem cardiac samples were collected from the left ventricle (LV) right ventricle (RV) or right atrium (RA) within 15 minutes via snap freezing in liquid nitrogen Approximately 50mg of tissue/sample was disrupted using a metal bead (Qiagen #69989) in a 2mL tubes with 500uL TRIzol (Ambion #15596018) using the TissueLyser LT (Qiagen) and RNA extraction performed according to TRIzol manufacturer's instructions. DNA removal was performed using Qiagen RNAse-Free DNase kit (Qiagen #79254) and purified using the RNeasy Mini kit (Qiagen, #74104) according to manufacturer's instructions. RNA quality was evaluated using a Nanodrop and an RNA Nano Chip (Agilent #5067-1511) on an Agilent Bioanalyzer. Libraries were prepared with Illumina Stranded Total RNA prep Ligation with Ribo Zero Plus kit, according to manufacturer's instructions. Libraries were sequenced using a paired-end 250bp Illumina NovaSeq 6000 S4 flow cell.  Approximately 50mg of tissue/sample was disrupted using a metal bead (Qiagen #69989) in a 2mL tubes with 500uL TRIzol (Ambion #15596018) using the TissueLyser LT (Qiagen) and RNA extraction performed according to TRIzol manufacturer's instructions. DNA removal was performed using Qiagen RNAse-Free DNase kit (Qiagen #79254) and purified using the RNeasy Mini kit (Qiagen, #74104) according to manufacturer's instructions. RNA quality was evaluated using a Nanodrop and an RNA Nano Chip (Agilent #5067-1511) on an Agilent Bioanalyzer. Libraries were prepared with Illumina Stranded Total RNA prep Ligation with Ribo Zero Plus kit, according to manufacturer's instructions. Libraries were sequenced using a paired-end 250bp Illumina NovaSeq 6000 S4 flow cell. 