Mouse E14.5 foetal liver lineage marker-neg/SCA-1-positive/c-KIT-positive (LSK) cells were sorted by FACS. RNA was extracted using the RNeasy Micro kit (Qiagen, 74004). Mouse E9.5 embryos were dissected and stored in 350 µl Zymo DNA/RNA shield solution (Zymo, R1100-50). RNA was extracted using the RNeasy Mini kit (Qiagen, 74104) by adding 350 µl RLT buffer (Qiagen, 79216) and following the manufacturer's instructions. Mouse RNA isolated from FACS-sorted E14.5 foetal liver LSK cells (30 ng) or Mouse E9.5 Embryos (300 ng) was used to generate barcoded sequencing libraries using a library construction kit (TruSeq RNA Library Prep Kit v2, Illumina RS-122-2001, RS-122-2002). Paired-end 75 bp RNA-sequencing (RNA-seq) short reads were generated using NextSeq500 (Illumina, 20024908).