Genomic DNA of A. alternata ATCC 66981 was extracted from a pure culture using the NucleoSpin plant II kit (Macherey-Nagel) quantified and quality checked using a NanoDrop 1000 instrument (VWR International) and Qubit 3.0 photometer, respectively. Genome sequencing was carried out on the MiSeq platform (Illumina) as follows: the sequencing library was built using the Illumina Nextera DNA XT kit and quality checked using Experion DNA 1k analysis (Bio-Rad Laboratories). Raw reads (read length of 2300 bp) were processed with the FASTQ preprocessing toolkit (Blast2Go Pro V5.2)