Total RNA was extracted with the Rneasy Plus Mini Kit (Qiagen) and QIAshredder following the manufacturer's protocol. Poly(A) mRNA was enriched using the NEBNext® Poly(A) mRNA Magnetic Isolation Module using ~1µg of total RNA as input following the manufacturer's protocol. The NEXTflex Rapid Directional RNA-Seq Kit 2.0 (PerkinElmer) was used to generate cDNA libraries utilizing the NEXTflex RNA-Seq barcodes accoridng to the manufacturer's protocol. Libraries were sequenced using a Nova-Seq X with 150bp paired end reads.