Cells were labelled with 4-thiouridine (4sU) one hour prior to harvest, lysed, and total RNA was isolated. Purified RNA were treated with iodoacetamide for alkylation of 4sU, amplified, and libraries were sequenced. Libraries were prepared using Lexogen Quantseq 3'mRNA-seq V2 library prep kit and sequenced using single-end reads on an Illumina NextSeq 2000.