GSM9206760: ko_mi_200_opn2_infarct; Mus musculus; RNA-Seq
Left ventricular tissues were dissected from the infarct zone (anterior wall) and remote zone (posterior wall) of each heart. One sample contains 2 to 3 mice. Total RNA was extracted using TRIzol reagent according to the manufacturer's protocol; For scRNA-seq, Whole hearts were enzymatically digested as previously described, and CD45+ immune cells were isolated by fluorescence-activated cell sorting (FACS) using a BD FACSAria III. Approximately 30,000 sorted cells were processed for single-cell suspension preparation and library construction using a 5′ transcriptome library kit according to the manufacturer's protocol. Polyadenylated RNA was enriched and converted into cDNA libraries using a commercial library preparation kit. Libraries were multiplexed and sequenced to produce 150-base pair paired-end reads.