GSM9376341: WCM1078 organoids, active compound, 24h, rep3, mRNA; Homo sapiens; RNA-Seq
RNA-seq: RNA was extracted using the RNeasy Kit (Qiagen) Standard Illumina library scRNA: 1x10e6 cells were harvested in PBS. SORT-seq was performed using Single Cell Discoveries (SCD) service. ATAC-seq: Performed from 50'000 cryo-preserved cells per condition. PRO-cap: Approximately 30 million cells were processed per sample. Library preparations for two biological replicates were performed separately. Cells were permeabilized, and run-on reactions were performed. After RNA isolation, two adaptor ligations and reverse transcription were performed with custom adaptors. Between adaptor ligations, cap state selection reactions were carried out using a series of enzymatic steps. RNA washes, phenol:chloroform extractions and ethanol precipitations were conducted between reactions. All steps were performed under RNase-free conditions.