scRNA-seq of adult mantle in Crassostrea gigas
A single-cell RNA-seq library was prepared using the BMKMANU DG1000 GEM Kit and Droplet Generation Equipment following the manufacturers protocol. Single-cell suspensions in PBS with 0.04% BSA were loaded onto the C1000 Chip to generate GEMs, where cells were lysed and RNA barcoded during reverse transcription. Amplified cDNA was used to construct libraries, which were quality-checked with Qubit 4.0 and Agilent 2100, and sequenced on the Illumina NovaSeq X Plus platform with 150 bp paired-end reads.