GSM9053675: 12mo bio rep 2; Mus musculus; OTHER
Cortical tissue was mechanically homogenized in a sucrose solution and nuclei were isolated through a iodixanol gradient and ultracentrifugation. CUT&Tag was performed with anti-H2BE antibody (Millipore ABE1384). Transposition reaction mix from Nextera Library Prep kit was used (Tagment DNA Enzyme 1 and Tagment DNA Buffer).