RNA-Seq of Myzus persicae from Physalis: pool of nymphs and adults
The libraries were constructed using the Takara SMARTer v3 Low Input Pico kit with U MI. The construction begins with the template-switching activity of reverse transcriptase to enrich for full-length cDNAs and to add defined PCR adapters to both ends of the first strand cDNA. The cDNA is enzymatically fragmented followed by library amplification and indexing of the libraries.