CKI_Sham (CRX2065867)
Single-cell suspensions were obtained by digesting the tissue with 2 mg/mL collagenase I, 4 mg/mL collagenase IV, 50 U/mL DNase I, and 10% Fetal Bovine Serum for 40 minutes at 37 degree Celsius. Single-cell RNA-seq library was then constructed by using the BD Rhapsody Single-Cell Analysis System according to the manufacturer's instructions. This process primarily involved using FastQC (v0.11.9) to check the base quality and adapter contamination of raw reads, ensuring that the Q30 base proportion is greater than or equal to 90%. Subsequently, the BD Rhapsody Analysis Pipeline (eg. version v1.6.0) was used to align the clean reads to the mouse reference genome (GRCm38), generating a "cell-by-gene" raw UMI count matrix based on barcode and UMI information.