16S rRNA V4-V5 amplicon sequencing of wheat rhizosphere soil (CM220, rep5)
Total genomic DNA extracted with FastDNA SPIN Kit for Soil. V4-V5 hypervariable region of 16S rRNA amplified with primers 515F (5-GTGCCAGCMGCCGCGG-3) and 907R (5-CCGTCAATTCMTTTRAGTTT-3). Sequenced on Illumina NovaSeq 6000 (PE250, SP-Xp).