SnRK1a_Dark_1
Seedlings were grown in glass tubes containing half-strength Murashige and Skoog (MS 1/2) medium. Plants were maintained in a growth chamber at 26 C under a 14 h light/10 h dark cycle, with light provided from 6:00 am to 7:00 pm at approximately 30 PAR. At the V1 stage (formation of the first leaf with a visible collar, ~7 days after germination), seedlings were transferred to complete darkness for 48 hours to mimic starvation. Seedlings were harvested at 9 days after germination, after 2 days of darkness.