GSM9306667: T cells from donor D2008, 5-FU-treated, ATAC; Homo sapiens; ATAC-seq
Tumor specimens were dissociated by a gentleMACS dissociator (Miltenyi Biotec) and cryopreserved. After thawing, CD3+ live cells were sorted by FACS and subjected to scATACseq sample preparation. PBL samples from the same patients were also sorted for live cells for scATACseq sample preparation. Libraries were prepared according to manufactures' instruction (single-cell 5' immune profiling and single-cell ATAC) (10X Genomics)