GSM8683822: ATAC_Caco-2_rep1; Homo sapiens; ATAC-seq
For ATAC-seq, cells are resuspended in cold lysis buffer and transposition is performed with buffer containing tagment DNA enzyme (Illumina). DNA is purified using the MinElute PCR purification kit (Qiagen). For ATAC-seq, libraries are amplified using NEBNext High-Fidelity 2 × PCR Master Mix (New England Biolabs). PCR products are purified using QIAquick PCR purification kit (Qiagen). Libraries are paired-end sequenced using Illumina sequencing (NextSeq500 Illumina).