GSM9307739: ATACseqI3; Homo sapiens; ATAC-seq
hSSCs were seeded into 96 well plates at 3000 cells per well onto Active Wnt3a platform or Inactive Wnt3a platform with 100 μl hSSC basal medium and cultured for 24h to allow cells to attach. After 24h, media was replaced with osteogenic media and cells were cultured for 48h. Cells were washed twice with PBS and trypsinized (Trypsin EDTA 0.05%) for 3min at 37C. 18 wells were pooled to obtain enough cells and counted as 1 biological replicate (n=4 per group). Samples were then processed for ATAC-seq using a commercial kit (Active Motif, ATAC-Seq Kit) according to the manufacturer's recommendation. Sample quality was assessed using the Fragment Analyzer 5200 (Agilent, software version: 3.1.0.12). Sequencing of the libraries generated was done on an Aviti sequencer (Element Biosciences), 50 million reads per library.