DNA-Seq of Chicken manure
This study investigated the bacterial community dynamics during continuous ammonia fermentation of organic solid wastes using sludge cake (SC) and chicken manure (CM) as substrates. Samples from the initial inoculum and fermentative broths (SC-100 and CM-300 at HRT 4 days) were collected for microbial analysis. The 16S rRNA gene V4 region was amplified using primers 27F and 800R, and sequencing was performed on the Roche 454 Genome Sequencer FLX Titanium platform