GSM9888060: Wild_Type; Mus musculus; RNA-Seq
Mouse esophageal epithelial cells were prepared as single-cell suspensions and enriched for EpCAM⁺ epithelial cells while excluding dead cells and immune cells by FACS. Libraries were generated with 10x Genomics Chromium (Single Cell 3′ V3/V3.1) targeting 8-12k cells per sample, sequenced on Illumina NovaSeq 6000 (2×150 bp, ≥50k reads/cell).