Transcriptomic assessment of parental and radioresistant human cell line models of prostate cancer
Transcriptomic assessment of parental and radioresistant human cell line models of prostate cancer
For each sample, pellets containing up to 10^6 cells were collected by trypsinisation and centrifugation, followed by snap-freezing on dry ice. Bulk RNA was extracted from pellets using Qiagen AllPrep DNA/RNA Mini kit (Cat. No. 80204), according to the manufacturer's instructions. Individual libraries were generated from 500ng total RNA using Lexogen's QuantSeq 3' mRNA-Seq V2 Library Prep Kit (FWD) for Illumina with UDI 12nt, according to the manufacturer's protocols. 