Bacterial transcriptome
A Library was constructed with TruSeqTM Stranded Total RNA Library Prep Kit, and dTTP was replaced with dUTP in the dNTPs reagent for synthesizing the second strand of cDNA, so that the bases in the second strand of cDNA contained A/U/C/G. Before PCR amplification, the second strand of cDNA is digested with the UNG enzyme so that the library contains only the first strand of cDNA.