Nanopore Amplicon gDNA seq of OMM2-5
DNA was extracted from cell line using the DNeasy Blood & Tissue Kit (QIAGEN), PCR amplification was performed on one mutational hotspot region in each SF3B1, SRSF2, GNA11 and GNAQ. PCR products were purified with AMPureXP beads (Beckmann Coulter) and sequenced using the SQK-RBK114.24 kit (ONT). Barcode: RB18