metagenomic of cruznema sp. ntm-2021: non-surface sterilized (control)
The sequencing library was prepared using full-length 16s sequence with barcodes embedded in the amplicon using M13 sequence primers and barcode kit (forward: bc1004 and reverse: bc 1051). Sample was sequenced on the Sequel system by PacBio (www.pacb.com). Raw sub-reads were processed through the SMRTlink (v11.0) Circular Consensus Sequences (CCS) algorithm to produce highly accurate reads with the quality above 40.