GSM8547214: H26/0.9 M NaCl/ 26h Repl 2; Haloferax volcanii; RNA-Seq
total RNA was isolated using phenol/chloroform extraction. Afterwards, the RNA was DNase-I (Thermo Fisher Scientific) digested and again purified with phenol/chloroform extraction. The RNA was shipped to StarSEQ GmbH (Mainz, Germany), where after another quality check the rRNA depletion (NEBNext rRNA depletion Kit) and Sequencing was done (Illumina Next Seq 500; 25 mio PE reads with 2x 150 nt). NEBNext Ultra II directional, dual index library