sequences of metagenome
A 3-month litter control experiment was carried out in three subtropical forest stands at Tianmuhu Forest Park. Soil physicochemical detection and metagenomic sequencing were combined to profile ARGs, MGEs and microbial communities. Multiple statistical models including NCM, co-occurrence network, GAM and PLS-PM were applied to analyze ARG assembly rules and driving factors, aiming to clarify how litter disturbance shapes soil resistome and identify core regulators of ARG dynamics.