MSCs (CRX1237402)
Under aseptic conditions, rats were sacrificed and discarding fat and fibrous tissues, synovium were dissected from the knee, cartilage tissues on the femoral condyle of the rat knee joint were separated. All the tissues washed twice with PBS, and cut into small pieces.Resuspended Single-cell were used to constructed libraries by Single Cell 3 Library & Gel Bead Kit v.3 according to the manufacturer's protocol (10X Genomics).