DNAseq of nine Seresta-selected and unselected Globodera pallida populations
DNAseq of nine Seresta-selected and unselected Globodera pallida populations
Nine Globodera pallida field populations were selected for four consecutive generations on the potato variety Seresta. DNA of approximately 40 cysts from the first and last generation of the selection experiment was used for DNA extraction. The 40 cysts were crushed by bead-beating, lysed (Holterman et al., 2006), after which DNA was extracted using phenol/chloroform/isoamyl alcohol.  1. DNA fragmentation 2. Size selection 3. End repair and \"A\" tailing 4. Adapter ligation 5. Product size selection 6. PCR reaction 7. Short-Insert Library QC