Amplicons ITS2 region of Aucoumea klaineana mycobiota : rootlets and rhizoplane
PCR products were purified with AMPure XP beads, and Illumina adapter overhangs with dual-index barcodes were added in a second PCR using overhang primers: forward (5 TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG[locus-specific]) and reverse (5 GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG[locus-specific]). Libraries were normalized to 7 ngL (Quant-iT PicoGreen) and quality-checked (QIAxcel). Final pools were quantified by qPCR (KAPA SYBR FAST) and sequenced on an Illumina MiSeq (v3 kit, 2  300 cycles) at 8.5 pM.