GSM9307426: NB-30kPa-3; Homo sapiens; RNA-Seq
Total RNA was isolated from cells using TRIzol reagent followed by purification with an RNA miniprep kit according to manufacturer's instructions. RNA was qualified and quantified using a Nanodrop One Spectorphotometer (Thermo Fisher). cells RNA was harvested using Trizol reagent. The RNA quality was checked by Bioanalyzer 2200 (Aligent) and kept at -80℃.The RNA with RIN >8.0 is right for cDNA library construction.