A375, MB4667, H2030-TGL, H2030-BrM3, MP41, HCC1954-LCC1, Sk-Mel-3, M040204, M040416, RPMI7951, HCC1954-LCC2, YUSUSA, CAPAN02, co-processed cultured human cancer cell lines used for EasySci single-cell RNA sequencing
Nuclei isolated from cultured human cancer cell lines were processed using the EasySci-RNA protocol. Reverse transcription, ligation-based barcoding, and indexed PCR amplification generated single-cell transcriptome libraries, sequenced on an Illumina NextSeq 1000 (paired-end 100 bp; dual 10 bp index reads).