GSM8941247: LMNA2; Homo sapiens; ATAC-seq
Briefly, the Cell membrane was permeabilized with nuclear lysis buffer. Then, cells were resuspended in 50uL of transposase reaction mixture. Transposition was carried out at 37°C for 22 min, followed by DNA purification with DNA Clean and Concentrator-5 (Zymo Research) according to the manufacturer's recommendation. Following purification, library fragments were PCR amplified with Nextera XT v2. adapter primers. Multiplexed and pooled library was sequenced on the Novaseq X. Plus with paired ends of 50 nucleotides per the manufacturer's instructions