Shotgun metagenomic sequencing of human stool sample
DNA was extracted from Stool swabs using the BiOstic Bacteremia DNA Isolation Kit (Qiagen).  Metagenomic sequencing completed using Illumina Nextera Kits and a NovaSeq 6000 sequencer for characterization of the gut microbiome in transplant recipients with and without graft versus host disease (GvHD).