GSM8717934: sorted pre-B cells, relapse, r3; Homo sapiens; RNA-Seq
Sorted cells were collected in 1 mL of cell-staining media containing RNAase inhibitors (VRC, Promega; RNAsin, New England Biolabs) and RNA was extracted using RNeasy Micro kit (Qiagen) following manufacturer's instructions. RNA libraries for RNA-seq were prepared using SMARTER Ultra low kit (Takara Bio/Clonotech Laboratories) for sequencing