GSM8486682: iKRAS_Day4_STAT3KO,scRNAseq, gene_exp; Mus musculus; RNA-Seq
Single cell suspensions were prepared by treating tumor tissues with 1x collagenase/hyaluronidase (StemCell Technology), 0.5 mg/ml Liberase-DL (Roche), and 0.1 mg/ml DNase I (Sigma) for 45 minutes at 37C with continuous mixing. Single-cell RNA sequencing was performed using 10X Genomics NextGEM v3.1 3' Gene Expression kits by the Cold Spring Harbor Laboratory Single-Cell Biology Shared Resource. Cells were loaded into the 10x Genomics Chromium Controller and libraries prepared according to the manufacturer's instructions. Libraries were paired end sequenced using the Illumina NextSeq2000 sequencing system with 28x10x10x90bp reads to a mean depth of ~45,000 reads per cell.