GSM8859482: KMS11, endogenous, ATF4 ChIP, biorep3; Homo sapiens; ChIP-Seq
Cells were fixed in 1% formaldehyde for 10 minutes (and double crosslinked with 3 mM EGS in PBS for 20min  for p300 and CBP before formaldehyde fixation), swelled in 0.5% SDS buffer then lysed in 0.5% SDS + 5% Triton buffer (2:1). Genomic DNA was sheared to 200-500bp by sonication. ChIP libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB)