smRNA-seq (Zymo libraries) of Montipora capitata: 14_4phf
150-190 ng of total RNA was used as input for miRNA library prep using the Zymo-Seq miRNA Library Kit (R3006) with 22 cycles of index PCR amplification (95C for 30 seconds, 62C for 30 seconds, 72C for 30 seconds). Small RNA-sequencing was performed on an Illumina NovaSeq X Plus in a 2x150 bp configuration at the Oklahoma Medical Research Foundation NGS Core. miRNA library prep on the majority of samples (n=3 per time point) failed to generate adequate libraries, leaving us with n=1 for miRNA sequencing. We suspect that the Zymo-Seq miRNA Library Kit (R3006) was insufficient and did not perform sequence amplification properly. Notably, this kit is no longer available for purchase on the Zymo website (https://www.zymoresearch.com/pages/ngs-library-prep-kits; accessed on 3/5/25), suggesting significant issues with its reliability.