BL_IVF_K27_2 (CRX1622224)
After removal of the zona pellucida by 0.8% acidic GMOPS, 10 blastocysts were used as a replicate. Approximately 1 ug of histone H3K4me3 antibody (Cell Signaling Technology, 9751T) or histone H3K27me3 antibody (Active motif, 39155) was used for the immunoprecipitation reaction. The ULI-NChIP-seq libraries were constructed using the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, E7645L) and Q5 High-Fidelity DNA Polymerase (NEB, M0491S). Quality control and sequencing were conducted by Berry, Novogene and ANOROAD Genomics Corporation.