Saccharomycopsis sp.
The concentration of the extracted DNA was determined using Qubit fluorophotometer, while its integrity was assessed via 1% agarose gel electrophoresis. Subsequently, Watchmaker DNA Library Preparation Kit (CAS: 7K0019-096) was utilized to prepare the DNA library. For each DNA sample, 200 ng were taken for library preparation. During this process, the DNA was enzymatically digested into fragments with an average size of 350 base pairs. Once the library was constructed, a preliminary quantification was conducted using Qubit 3.0 fluorometer. This was followed by the detection of fragment size distribution using a fragment analyzer. After confirming that the library met the expected size and peak distribution criteria, a qPCR quantification was performed using ABI Quant Studio 12K Flex to further ensure the quality of the library. Finally, sequencing was carried out on the DNBSEQ-T7 platform, employing the double-end sequencing program (PE) to obtain 150bp double-end sequencing reads.