GSM9207134: CD8+CD90.1+CD44+CD62L- transgenic TCRTAG CD8 T cells isolated 30d post activation in LMTAG infected spleens, rep 2; Mus musculus; RNA-Seq
CD8 T cells were sorted directly into Trizol LS and frozen. Total RNA was extraced from sorted cells using the Rneasy Micro kit (Qiagen)and amplified using the SMART-Seq v4 UltraLow Input RNA Kit (Clontech). Libraries were prepared using NEBNext polyA mRNA sample kits (NEB).