Multi-Tissue RNA-Seq Analysis in Mini Pigs: Body Size and Tissue-Specific Comparisons
Multi-Tissue RNA-Seq Analysis in Mini Pigs: Body Size and Tissue-Specific Comparisons
To identify tissue-specific body size-related genes, approximately 10 samples representing 5 different tissues were collected from MiniPig individuals with varying body sizes. This comprehensive sampling aimed to capture the relationship between tissue expression patterns and body size variations. All experimental procedures were thoroughly reviewed and approved by the National Institute of Animal Science (NIAS) under approval number NIAS20181295. Total RNA was extracted from tissue samples using the QIAzol® Lysis Reagent (Qiagen, Cat. No. 79306) and the RNeasy® Mini Kit (Qiagen, Cat. No. 74106) following the manufacturer's protocol. RNA concentration and purity were assessed using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, USA). Only samples with sufficient quality were used for downstream applications. Individual libraries were generated using Illumina TruSeq™ RNA Sample Preparation Kit (Illumina, San Diego, CA, USA).