GSM9752693: S26_0106_vhd_d; Mus musculus; OTHER
At experimental end point, Lung lobes are collected and fixed in 4% PFA, and were transferred to 70% ethanol after 24 hours of fixation. The samples were then processed and embedded in paraffin. 5 µm thick consecutive sections were collected from the paraffin blocks of lung samples, using a microtome (Reichert-Jung 2030 Biocut Manual Microtome). Selected sections were stained with Hematoxylin and Eosin (H&E). All steps for tissue preparation and staining were carried out following 10X visium HD guidelines. Spatial transcriptome sequencing libraries were prepared with 10X Genomics Visium HD Spatial gene expression Slides & Reagent Kit according to manufacturer's protocol. Mouse v2 probe set was used for hybridization.