GSM9736960: Individual ID 8438, no stimulus, baseline; Melospiza melodia; RNA-Seq
Total RNA was extracted from 20ul of whole blood using a RiboPure RNA Purification Kit (Ambion). After extraction, DNAse I was added and samples were incubated at 37°C for 30 min to remove genomic DNA from the RNA. Though RNA studies of human blood typically involve hemoglobin depletion, we opted instead to increase the number of reads, as there are currently no probes available for birds. Library preparation was conducted with a KAPA HyperPrep (Van Kets et al., 2014) and included mRNA poly A enrichment. Paired end sequencing of 150bp reads was conducted using 3 lanes of a NovaSeq X Plus 10B.