GSM8992948: BMDMs, LPS, rep 1; Mus musculus; OTHER
H3K27me3 profiling was performed in BMDMs using CUT&RUN kit (Epicypher, #14-1048) The DNA fragments were repaired, A-tailed and further ligated with Illumina adapter. The final DNA library was obtained by size selection and PCR amplification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina platforms, according to effective library concentration and data amount required.