TDp3G3 (CRX2193013)
Based on the polyA tail structure characteristic of its mRNA, olig dT was first used to enrich mRNA from total RNA. The purified mRNA was randomly fragmented using divalent cations, and double-stranded cDNA was synthesized with the fragmented RNA as the template and random oligonucleotides as the primers. The purified double-stranded cDNA underwent end repair/dA-tailing, followed by sequencing adapter ligation. The ligation products were purified and subjected to fragment size selection to obtain cDNA fragments of the desired size. Subsequently, PCR amplification was performed, and the PCR products were purified again to finally obtain the sequencing library.