GSM8545994: Uterus, PNA, normal diet, stimulated, rep 2; Mus musculus; RNA-Seq
Tissues were stored in RNAlater and in -80°C until processing. RNA extraction and DNase treatment of was performed using the RNeasy Mini kit (Qiagen) according to the manufacturer's instructions. Isolated RNA was submitted to Novogene Europe for further processing for RNA sequencing (RNAseq). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library. For the non-directional library, it was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. For the directional library, it was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification