GSM8718491: WT-HFD - TEN_025_12 (GE); Mus musculus; RNA-Seq
RNA was harvested using TRIZOL (ThermoFisher/Invitrogen, 15596026 or 10296028) according to the manufacturer's protocol. Standard: 500 ng total RNA is used for rRNA-depletion (NEB, E6310), followed by directional library synthesis (NEB, E7420). Poly-A purification: Poly-T column kit (NEB, E7490), followed by NEB kit (E7420).