GSM8507771: Mix7_1-31_R2_P6_G_pool2; Homo sapiens; RNA-Seq
Cells were lysed and RNA was extracted according to BioSpyder's protocol Total RNA input of 100 ng was used to build whole transcriptome Templated Oligo-Sequencing libraries using the TempO-Seq Human Whole Transcriptome 2.1 kit (BioSpyder, Carlsbad, CA); RNA was diluted to 50 ng/ul in 1 x TempO-Seq lysis buffer and 2 ul of this mixture was used as input. Briefly, detector oligos (DO) targeting whole transcriptome were annealed to mRNA. Each transcript is targeted by a pair of DOs. Bound DOs were ligated to form one contiguous sequence, while unbound DOs were eliminated by nuclease digestion. The ligated DOs were indexed and amplified by PCR, after which 96 [?] individual libraries were pooled to create a single pool for sequencing on the [instrument] (Illumina). A 96-sample Human Whole Transcriptome Template Oligo-Sequencing (TempO-Seq) kit, which contains 50 nt detector oligos that target mRNA transcripts, was used to generate sequencing libraries.