GSM9256219: Day 0,Male,Replicate 3; Mus musculus; RNA-Seq
Approximately 50 mg of snap-frozen cardiac tissue was processed using either the 10x Genomics Nuclei Isolation Kit (RNA samples) or the 10x Genomics Sample Fixation and Preparation Kit with Miltenyi OctoMACS dissociation. Nuclei or fixed cell suspensions were assessed with AO/PI on the LunaFx7 automated cell counter for quality and debris content. Libraries were prepared using 10x Genomics Chromium chemistry