GSM9821017: Atherosclerosis1; Homo sapiens; RNA-Seq
PBMC samples (four atherosclerosis and four sex- and age-matched healthy controls: A. Healthy) were processed for scRNA-seq using the 10x Genomics Chromium Single Cell 3' v4 platform, following the manufacturer's protocol. Briefly, after thawing and washing, cells were resuspended in PBS containing 0.04% BSA, counted, and assessed for viability. Cell suspensions were loaded into the Chromium Controller to generate Gel Bead-In-Emulsions, followed by reverse transcription, cDNA amplification, and library construction using the Single Cell 3' v4 Reagent Kit. Final libraries were quality-checked using an Agilent Bioanalyzer and quantified by qPCR. Sequencing was performed on an Illumina NovaSeq X Plus system (NovaSeq X Series Control Software v1.2.2.48004) using a 151 nt (Read 1) – 10 nt (Index 1) – 10 nt (Index 2) – 151 nt (Read 2) configuration on a '25B' mode flow cell.