16S rRNA amplicon long-reads of gut microbiote in swine dysentery
The 27F (AGRGTTYGATYMTGGCTCAG) and 1492R (RGYTACCTTGTTACGACTT) universal primers were used to amplify the full-length 16S rRNA gene from the genomic DNA. Both the forward and reverse 16S primers were tailed with sample specific PacBio barcode sequences to allow for multiplexed sequencing for Multiplexed SMRTbell Library Preparation and Sequencing protocol (Part Number 101599-700 Version 04, PACBIO).