GSM8718901: 18S_H-RF7-T10; Ovis aries; OTHER
Total genomic DNA was isolated from 1 mL of freeze rumen fluid using TIANamp Stool DNA Kit (DP328, Tiangen) according to manufacturer's instruction. Amplicon sequencing of rumen 18S rRNA sequencing were performed according to Illumina  protocols with all amplicon libraries being sequenced on the NovaSeq 6000 platform (Illumina, San Diego, CA) with 250-base-paired end reads.