Fastq data of UCE captured sequence of B.s.brevipes_S23
DNA from fresh blood and tissue was extracted using Qiagens DNeasy kit and protocol. Extracted DNA was sheared to a target size of 550bp using a Covaris sonicator set at 75 W peak incident power, 10% duty factor, and 200 cycles per burst for 40 seconds. Samples were then cleaned with QuantaBio sparQ Magbeads and Kapa Biosystems HyperPrep kits were used for end-repair, adapter ligation, and initial amplification (6-8 cycles of PCR depending on sample DNA concentration). New England Biosciences NEBNext Multiplex Oligos for Illumina were used as primers. The samples were hybridized to baits from Arbor Bioscience MyBaits Tetrapods kit v5.2 to target 5060 UCE loci, and subsequently amplified for an additional 10-12 cycles (Faircloth et al., 2012). Libraries were pooled and amplified again for three additional PCR cycles to increase DNA concentration. The pooled libraries were sequenced at Novogene facilities using the NovaSeq PE150 platform.