PacBio Onso short-read whole genome sequencing of Staphylococcus warneri strain ABSKSKLAB05
Whole genome sequencing of Staphylococcus warneri strain ABSKSKLAB05 isolated from rhizosphere soil collected from agricultural fields in Telangana, India. Genomic DNA libraries were prepared using the QIASeq FX DNA Library Preparation Kit involving enzymatic fragmentation, end repair, A-tailing, and ligation of indexed adapters followed by limited-cycle indexing PCR amplification and purification. The libraries were converted to PacBio Onso-compatible libraries and sequenced on the PacBio Onso platform to generate high-accuracy paired-end short reads. These short reads were used together with long-read data to perform hybrid genome assembly and polishing of the bacterial genome, enabling reliable genome annotation and identification of biosynthetic gene clusters involved in antimicrobial and other therapeutically relevant natural product biosynthesis.Authors: Anushka Bhrdwaj (Department of Bioinformatics, Alagappa University, Karaikudi - 630003, Tamil Nadu, India), Dr. Anuraj Nayarisseri (In silico Research Laboratory, Eminent Biosciences, Indore - 452010, Madhya Pradesh, India), Prof. Sanjeev Kumar Singh (Department of Bioinformatics, Alagappa University, Karaikudi - 630003, Tamil Nadu, India)