GSM8993166: PRDM16cKO snRNA-seq; Mus musculus; RNA-Seq
Mesenteric perivascular adipose tissue was microdissected from mice and snap-frozen in liquid nitrogen. Snap-frozen fat pads were homogenized in TST buffer (146 mM NaCl, 21 mM MgCl2, 10 mM Tris-HCl pH7.5, 1 mM CaCl2, 1% Molecular Grade BSA, 0.2 U/µL RNase inhibitor, 0.1% Tween-20, in Ultrapure H2O) using a GentleMACS Tissue Dissociator. Homogenized tissues were then centrifuged. Pellets that contained nuclei were collected and further purified with an iodixanol gradient. snRNAseq library was prepared using 10x Chromium Next GEM Single Cell 3' kit v3.1 16 rxns (PN-1000268), following the manufacturer's protocol.