GSM9461752: CUT&Tag, KDM5b Hom1; Mus musculus; OTHER
Tissue was homogenised and nuclei extracted as described in dx.doi.org/10.17504/protocols.io.36wgq4965vk5/v2. Libraries were prepared using the CUTANA CUT&Tag kit (Primer set 1; Epicypher). K-MetStat spike-ins were added to each sample prior to  the addition of the antibody. Anti-H3K4me3 primary antibody (Epicypher, #13-0060) was used at a concentration of 1:50 (0.5 ug). Rabbit IgG was used as a negative control. Quality and quantity of DNA was assessed using Agilent Tapestation and QuBit Flex (Invitrogen)