=_xlfn.CONCAT("sub-cellular RNAseq of rattus norvegicus: hippocampal pyramidal neuron #", F11, " ", LOWER(B11) )
A micropipette was used to aspirate the soma, which was deposited into a tube containing first strand synthesis buffer and RNase inhibitor and placed on ice. A separate micropipette was used to aspirate the dendrites, which were deposited into a separate tube as above. Samples underwent first strand synthesis within 30 minutes of collection. Poly-adenylated RNA was amplified using three rounds of the aRNA in vitro transcription-based amplification method, as described in (Morris, Singh, and Eberwine 2011). The quality and quantity of the amplified RNA was verified using a Bioanalyzer RNA assay. Strand-specific sequencing libraries were prepared using the Illumina TruSeq Stranded kit according to the manufacturer's instructions, except that the initial poly-A capture step was skipped because the aRNA amplification procedure already selects for poly-adenylated RNA. Samples were sequenced on a  NextSeq (75bp paired-end) to an average depth of 30 million reads.