GSM9753297: XΔ2NipD - R1; Drosophila melanogaster; RNA-Seq
For each sample, approximately 5 pairs of ovaries from 0-1 day old females were dissected in RNase-free 1x PBS and flash frozen in liquid nitrogen until RNA extraction. RNA extraction for each replicate was performed using the RNeasy RNA extraction kit (Qiagen). Samples were treated with DNase post RNA extraction and purified using an RNA purification kit (Promega). RNA concentrations were assessed using a Nanodrop as well as a Qubit RNA analyzer for sample quality and RIN scores. Illumina Truseq Total RNA (ribosomal depletion)