RNA-Seq of Oryzias melastigma: Mid gastrula stage, replicate 2
Libraries were constructed from total RNA extracted from whole embryos of Oryzias melastigma. mRNA was enriched using oligo(dT) magnetic beads. The mRNA was fragmented and reverse-transcribed into cDNA using random hexamers. Adapters were ligated to the cDNA fragments, followed by PCR amplification. The resulting libraries were sequenced on an Illumina NovaSeq 6000 platform with 150 bp paired-end reads.