GSM8682955: siZC3H4 Repl. 2; Mus musculus; RNA-Seq
Cells were collected in Trizol, ERCC spike-in was added and RNA was extracted using DirectZol columns. Genomic DNA contamination removed using DNAse, and RNA was cleaned up by adding Trizol and extracting RNA using DirectZol columns again. All samples had RIN scores of >9. 800ng purified RNA was used as input to the Illumina TruSeq stranded total RNA kit with Ribo-Zero Gold rRNA depletion. The manufacturer's protocol was followed, but Superscript III was used for first-strand synthesis.