Whole genome sequencing of Candida albicans 101
High molecular weight DNA was sheared with Megaruptor (Diagenode, Denville, NJ, USA) to obtain 10-15 kb fragments. After shearing the DNA size distribution was checked on a Fragment Analyzer (Advanced Analytical Technologies, Ames, IA, USA). 1.8g of the DNA was used to prepare a SMRTbell library with the PacBio SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences, Menlo Park, CA, USA) according to the manufacturer's recommendations. The resulting library was pooled with other libraries processed the same. The pool was size selected on a Blue Pippin system (Sage Science, Inc. Beverly, MA, USA) for molecules larger than 6 kb. It was sequenced with v2.2/v2.0 chemistry and adaptive loading on a PacBio Sequel II instrument (Pacific Biosciences, Menlo Park, CA, USA) with 30h movie length, pre-extension time of 2h using one SMRT cell 8M.