GSM9887455: 7d culture of Sox2::mcherry+ in FGF, sorted for Sox2::mcherry+ rep2; Mus musculus; RNA-Seq
Bead-based extraction approach from cells preserved in Zymo DNA/RNA Shield. Library preparation by Plasmidsaurus: Poly-A mRNA capture and reverse transcription for cDNA synthesis: oligo-dT primer to capture just the mRNA (contains sample barcode, UMI, and Read 1 sequences). Second strand synthesis and tagmentation: double stranded-cDNA is generated. Tagmentation creates fragments and incorporates Read 2 sequence. Illumina library amplification: adds unique dual indices (UDI = i5, i7) and P5/P7 sequences.