HCT116-RNAseq
After the sample is qualified, the library is constructed, and the main process is as follows: 1) mRNA enrichment of totaIRNA by mRNA enrichment method: mRNA with polyA tail enrichment is carried out with magnetic beads with Oligo dT. 2) Fragment the obtained RNA with interrupted buffer, reverse transcribe random N6 primers, and then synthesize cDNA two strands to form double-stranded DNA 3) Flatten the synthetic double-stranded DNA ends and phosphorylate at 5 ends, form a sticky end protruding from an "A" at the 3' end, and then connect a bubbling adapter with a protruding "T" at the 3" end