GSM8546118: TREAT 2+3; Mus musculus; OTHER
FFPE tissue sections were prepared on slides according to the manufacturer's instructions (10x Genomics, CG000518). Specific probes hybridized to target RNA molecules within the tissue sections. The probes were designed to bind to specific mRNA targets and were tagged with unique barcodes for spatial resolution. Following hybridization, unbound probes were washed away, and the tissue sections were ready for downstream processing. fter hybridization of the barcoded probes to the target RNA in the FFPE tissue sections, the bound probes were ligated to form a continuous sequence that includes the spatial barcodes and target-specific sequences. Ligated probes were amplified through PCR to generate sufficient material for sequencing. The amplified products were purified and prepared for library construction using standard protocols (10x Genomics, CG000495). The final libraries were quantified by qPCR and quality-checked using a bioanalyzer.