GSM9535286: Whole testis, Bearded dragon, scRNAseq, Rep2; Pogona vitticeps; RNA-Seq
Testicular disaggregation from tammar wallaby and bearded dragon were conducted as previously described (1). An aliquot of the single-cell solution was loaded into a ChromiumTM Single Cell Chip (10x Genomics®), aiming to recover between 5,000 and 6,000 cells. Libraries were prepared following manufacturer's instructions and sequenced on Illumina NovaSeq 6000 (50bp pair ends).