To explore the key bacterial species involved in liver regeneration (CRX857739)
Bacterial genomic was extracted from mice feces using a DNA extraction Kit (Mabio, Guangzhou, China) according to the manufacturer's protocol. DNA (5 ng/mL) was used for quantitative real-time polymerase chain reaction. Polymerase chain reaction was performed for the variable region 4 (V4) of the bacterial 16S rRNA gene with primers V4F (50-GTGTGYCAGCMGCCGCGGTAA-30) and V4R (50-CCGGACTACNVGGGTWTCTAAT-30). For microbial diversity analysis, DNA sequencing was performed on the Illumina sequencing platform and raw data were analyzed using QIIME2 to identify gut microbial profiles.