GSM9821028: si-IFITM3-RH; Chlorocebus sabaeus; RNA-Seq
cells were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. illumina TruSeq RNA Sample Prep Kit il mRNA was enriched from total RNA using oligo(dT) magnetic beads. The enriched mRNA was fragmented into 200-300 bp fragments using fragmentation buffer. First-strand cDNA was synthesized using random hexamer primers and reverse transcriptase, followed by second-strand cDNA synthesis using DNA polymerase I and RNase H. The double-stranded cDNA was subjected to end repair, A-tailing, and ligation with Illumina sequencing adapters. The ligated products were purified and amplified by PCR for 12-15 cycles. The final libraries were quantified using Qubit 3.0 Fluorometer and validated for size distribution using Agilent 2100 Bioanalyzer.