DNA methylation of endosperm of Panax notoginseng seeds at 50d-2 (CRX763814)
Genomic DNAs were fragmented into 100-300bp by Sonication(Covaris,Massachusetts,USA) and purified with MiniElute PCR Purification Kit(QIAGEN,MD,USA).The fragmented DNAs were end repaired and a single A nucleotide was added to the 3' end of the blunt fragments. Then the genomic fragments were ligated to methylated sequencing adapters. Fragments with adapters were bisulfite converted using Methylation-Gold kit(ZYMO,CA,USA), unmethylated cytosine is converted to uracil during sodium bisulfite treatment.