Whole genome sequencing of evolved Pseudomonas spp.
We extracted the DNA from the ancestors and all 72 populations using the Qiagen Blood and Tissue Kit for DNA Extraction. The genomic DNA concentration was quantified using Qubit dsDNA HS assay kits. The DNA was prepared for short read sequencing using the Illumina DNA Prep kit. The Illumina librariers were each uniquely barcoded, multiplexed, and sequenced using the Illumina NextSeq 2000