ITS of microbial biofilm from maple (acer saccharum) leaves
DNA from microbial biofilms on submerged maple (acer saccharum) leaves was extracted using DNeasy PowerSoil Pro Kits (Qiagen). The fungal ITS region was amplified using PCR with primers ITS1F and ITS2Rev (White et al 1990), and pooled amplicons were sequenced on an Illumina NextSeq instrument using paired-end sequencing.