RIL-seq on CsrA RNA binding protein in EPEC bacteria
RIL-seq on CsrA RNA binding protein in EPEC bacteria
Single colonies of wild type EPEC (E2348/69) and CsrA-3XFLAG (NN6861) strains were inoculated from a fresh LB plate in liquid LB and grown over-night without shaking at 37Â°C. Bacteria were then diluted (1:50) in 200ml of pre-wormed DMEM and grown to OD600 0.4. The bacteria were harvested and subjected to RIL-seq experiments as described (Melamed et al., 2018; Melamed et al., 2016) with modifications to several steps in the protocols RNA was extracted using TRI Reagent (Sigma) as described (Faigenbaum-Romm et al., 2020) Libraries according to (Shishkin et al., 2015) were constructed with several modifications (Melamed et al., 2018).