GSM9256814: E_mu-Myc lymphoma from the Lymphatic model of dissemination1; Mus musculus; RNA-Seq
After dissection, lymph nodes were disrupted into single cell suspension using sterile nuclease free tools. Following Eu-myc lymphoma sorting in sterile condition, Total RNA was extracted using the Direct‐zol RNA Miniprep kit (R2051, Zymo Research, Irvine, CA, USA). RNA quality and concentration were assessed using the Agilent 2100 bioanalyzer (Agilent Technologies Inc., Santa Clara, CA, USA). NEBNext Ultraexpress RNA Library Prep Kit for Illumina (E3330S - New England BioLabs Inc.) was used with the NEBNext Multiplex Oligos for Illumina (New England BioLabs Inc.) and NEBNext® Poly(A) mRNA Magnetic Isolation Module for cDNA synthesis with the addition of barcode sequences. The pre-pool sequencing was performed using the NextSeq2000 (Illumina, San Diego, CA, USA) with the P2 reagents kit V3 (100cycles; Illumina). Samples were processed starting from stranded, single-ended 120bp-long sequencing reads.