GSM9308630: WT larvae PBS-H3K4me3 ChIP; Danio rerio; ChIP-Seq
WT and cldc1-/- Zebrafish larvae at 2 dpf received β-glucan training through yolk sac microinjection. Four days post-training, larval cells were collected and cross-linked with formaldehyde (37%, Sigma) at 37 °C for 10 min. After fixation, cells were resuspended in SDS buffer and sonicated. Chromatin immunoprecipitation (ChIP) was conducted using a commercial ChIP Assay Kit (Beyotime) with an H3K4me3-specific antibody (GeneTex), following the manufacturer's protocols. DNA was recovered by phenol/chloroform precipitation and library preparation was performed using the NEBNext Ultra II DNA Library Prep Kit prior to Illumina sequencing (Novaseq PE150).