AT-QC-28 (CRX2027551)
Nanopore sequencing using Ligation Sequencing Kit 1D (ONT,Beijing, China, Cat. SQK-LSK109) following the instructions. Briefly, the cDNA fragments were end-repaired and added dA-tailed using the Ultra II End Prep module (NEB, Ipswich,Massachusetts, USA, Cat. E7546) and then tethered to 1D adapter by using Quick Ligation Module (NEB, Cat. E6056). After that, each cDNA library was loaded into 1 FLOPRO002 flow cell and sequenced on PromethION.Beta.