L1EJE0802110-JKZ2_23_1 (CRX2070111)
Library construction criteria included total RNA 1ug, concentration 50ng/uL, and Optical Density at 260 nm and 280 nm (OD260/280) between 1.8-2.2.mRNA was enriched using magnetic beads with Oligo(dT), randomly fragmented to approximately 300bp using fragmentation buffer, and reverse transcribed to synthesize first- and second-strand cDNA.