GSM9886474: SPRITE cerebellum, P6, rep1, batch3; Mus musculus; OTHER
Cerebella were microdissected and fixed in 1% formaldehyde in PBS. The cell lysis was treated with DNase, followed by sonication. The lysate was then treated with Proteinase K and de-crosslinked. The average DNA fragment size was about 200 bp, and 10 billion molecules of DNA-protein complexes (about 6000 cells) were coupled with 1.7 ml of NHS-Activated Magnetic Beads (Pierce) per experiment. SPRITE libraries were generated after five rounds of split-pool barcoding reactions using DPM, odd, even, odd, and terminal odd barcodes.