RNAseq of Erwinia amylovora on glandular trichomes on apple leaves
The RNA went through a bacterial and plant rRNA depletion using NEBNext rRNA Depletion Kit (Bacteria) and TruSeq Stranded Total RNA with Ribo-Zero Plant. The RNA was then sequenced using illumina NovaSeq X Plus on 25 B flow cell, paired end reads, 2x150bp and were originally sequenced at 25 million reads, but was sent back for resequencing at 200 million reads.