Brca1 KO CH12 cells (replicate 2)
100 ng of isolated gDNA were used in a reaction (25 ml). The mSm FW (GGAGGGACCCAGGCTAAGAAGGC), is mixed in combination with mSa REV (GCAAGCAGTGGACCCAAAGACGAGAGG), mSg2bc REV (CTGATGGGGGTGTTGTTTTGGCTG), mSg3 REV (GGGCTGTTGTTGTAGCTGCAAGATAGG) or mSg1 REV (GCTCAGAGTGTAGAGGTCAGACTGC) independently. The PCR protocol consists in 30 cycles of 95C for 40s, 60C for 30s, 65C for 3 min; and the last elongation step at 65C for 10 min using a ramping of 2C/s