ITS amplicon sequencing of fungal metagenome: Betula platyphylla leaf litter
Total genomic DNA was extracted from Betula platyphylla leaf litter samples collected from the Wudalianchi volcanic lava plateau using a fungal DNA extraction kit. The internal transcribed spacer (ITS) region of fungi was amplified by PCR with specific primers. The PCR products were purified, and sequencing libraries were constructed using the Illumina Nextera XT Library Prep Kit following the manufacturer's instructions. Libraries were sequenced on an Illumina MiSeq platform with paired-end (2306 bp) mode.