GSM9258327: TotalRNA_32_R1.trimmed.fastq.gz; Homo sapiens; RNA-Seq
Total RNA was extracted from cultured A549 cells (2D) or lung tissue fragments (3D) using the RNeasy Mini Kit (QIAGEN) following the manufacturer's instructions. RNA quality and concentration were assessed by NanoDrop spectrophotometry. RNA sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) with poly(A) RNA enrichment, following the manufacturer's instructions.