ds15-Hi-C-1 (CRX799067)
Samples were cross-linked under vacuum infiltration with formaldehyde and quenched with glycine. The cross-linked samples were subsequently lysed. Endogenous nuclease were inactivated with SDS, then chromatin DNA were digested by 100U MboI (NEB), and marked with biotin-14-dCTP (Invitrogen) and then ligated by 50U T4 DNA ligase (NEB). After reversing cross-links, the ligated DNA was extracted through QIAamp DNA Mini Kit (Qiagen) according to manufacturers' instructions. Purified DNA was sheared to 300- to 500-bp fragments and were further blunt-end repaired, A-tailed and adaptor-added, followed by purification through biotin-streptavidin-mediated pull-down and PCR amplification.