RNA-Seq of Spen mutant and wildtype ESCs
XCI was artificially induced (Dox-inducible Xist) in mouse ESCs containing different mutations in SPEN and wild-type hybrid TXXGFP/Cast cells. After 4 d induction, RNA was extracted using RNeasy Kit (QIAGEN), library prep was done using Illumina Stranded mRNAPrep, Ligation (Illumina). Libraries were sequenced on a NovaSeq X Plus (Illumina).