GSM9736036: LLC wild-type tumor, biol. rep. 3; Mus musculus; RNA-Seq
Tumor samples were dissociated and lysed in TRIzol reagent (Invitrogen). RNA was separated into the aqueous phase by the addition of chloroform, precipitated using absolute ethanol, washed, and resuspended in RNase-free water. RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies). RNA libraries were prepared from total RNA using the Stranded mRNA Prep Ligation kit (Illumina) following the manufacturer's instructions. Libraries were sequenced on an Illumina NovaSeq 6000 (S1 flow cell) in paired-end 100 bp mode, targeting 50 million reads per sample.