GSM9464125: Control rat intestinal tissue RNA-seq_4; Rattus norvegicus; RNA-Seq
Total RNA was extracted from intestinal tissue samples using standard RNA extraction procedures. RNA purity was assessed using NanoDrop spectrophotometry, and RNA integrity was evaluated using the Agilent 5400 Bioanalyzer. Poly(A)+ mRNA was enriched from total RNA using oligo(dT) magnetic beads and fragmented. First-strand cDNA was synthesized using random hexamer primers, followed by second-strand synthesis. After end repair, A-tailing, and adaptor ligation, fragments of approximately 250–300 bp were selected and amplified by PCR to generate the final sequencing libraries.