GSM9053983: yolksac, infected live1; Gallus; RNA-Seq
Yolk sac and embryonic liver tissues were collected and immediately placed in 1 mL of TRIzol reagent (Invitrogen, CA, USA). Tissue homogenization was performed using the Precellys® Evolution Touch homogenizer (Bertin Technologies, France) with ceramic beads at 8000 rpm for two cycles of 30 seconds each, separated by a 15-second pause. RNA extraction was carried out using the phenol: chloroform: isoamyl alcohol method (25:24:1), followed by isopropanol precipitation. Non-strand-specific cDNA libraries were constructed according to the manufacturer's protocol for the NEBNext® Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, MA, USA).