GSM9054259: Treg group 9 day aftre TAC surgery, #3; Mus musculus; RNA-Seq
The myocardial tissue was sacrificed and the left ventricle was cut into small pieces, immediately sealed in a polypropylene tube containing RNA later, and stored at -80°C. Total RNA was extracted from frozen myocardial tissue samples using the miRNeasy Mini Kit (QIAGEN, Hilden, Germany), and RNA quality was assessed by Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). RNA sequencing libraries were constructed using the TruSeq Stranded mRNA (Illumina, San Diego, CA, USA) and subjected to a paired-end 101bp sequencing run on the NovaSeq X plus (Illumina).