Small RNA sequencing of carnation stunt-associated viroid (CarSAV) infected carnation (Dianthus caryophyllus L. var. Tanga) samples
Small RNA sequencing of carnation stunt-associated viroid (CarSAV) infected carnation (Dianthus caryophyllus L. var. Tanga) samples
Samples were taken from CarSAV infected as well as from healthy culture for total RNA extraction.  Carnation (D. caryophyllus L. var. Tanga) plantlets were micropropagated and cultured based on the method Piquares and co-workers (2002). The extraction of small RNA and small RNA enrichment was carried out based on the method Cárdenas et al. (2011). For miRNA-Seq library construction, NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, Ipswich, MA, USA) was used according to the manufacturer's protocol. The quality and quantity of the library were determined by using a High Sensitivity DNA1000 ScreenTape system with 2200 Tapestation (Agilent Technologies, Santa Clara, CA, USA) and dsDNA HS Assay Kit with Qubit 3.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA).