Short-read whole-genome sequencing of Sporosarcina sp. A2 (14-04-2021)
Total DNA was extracted from an overnight culture obtained from a single colony of strain A2 using Proteinase K/SDS treatment and Genomic DNA Clean & Concentrator-10 Kit (Zymo Research). The DNA was fragmented using a Covaris S220 focused ultrasonicator (Covaris) for a target fragment length of 550 bp. Fragmented DNA was then used as input to prepare Illumina MiSeq-compatible DNA libraries for 250 bp paired-end read sequencing using the TruSeq DNA Nano Low-Throughput Library Prep Kit as per the manufacturer's reference guide. The resultant library was sequenced on the Illumina MiSeq system as one of the several individually barcoded pooled libraries using 500-cycle MiSeq Reagent Kit v2. PhiX sequencing control was used as a spike-in.