GSM9347034: Control1 tumor biol rep 1; Homo sapiens; RNA-Seq
Tumors were dissected and homogenized. Total RNA was isolated using the KingFisher Apex automated system (ThermoFisher) with Zymo Direct-zol Mag-Bead chemistry. RNA purity and integrity were verified using the Agilent Fragment Analyzer. Only high-quality RNA was used for library preparation. RNA samples were submitted to Novogene. Poly-A–selected, non-directional cDNA libraries were prepared according to the Novogene standard mRNA-seq workflow. Libraries were sequenced on the Illumina NovaSeq X Plus platform.