SK-UT-01, SK-NEP-01, Hek, SK-OV-03, SK-CO-01, OS252, M141207, 786-M2B, co-processed cultured human cancer cell lines used for EasySci single-cell RNA sequencing
Nuclei isolated from cultured human cancer cell lines were processed using the EasySci-RNA protocol. Reverse transcription, ligation-based barcoding, and indexed PCR amplification generated single-cell transcriptome libraries, sequenced on an Illumina NextSeq 1000 (paired-end 100 bp; dual 10 bp index reads).