GSM8825424: Whole lung tissue in WT mice with bleomycin-induced pulmonary fibrosis; Mus musculus; RNA-Seq
After anesthetizing the mice, lung perfusion was performed by flushing the heart with saline to remove blood from the lung tissue. After perfusion, the lungs were excised, the right lung was dissected, and finely minced using scissors. Tissue fragments from three mice were pooled and enzymatically digested with a mixture of Dispase, Collagenase, Elastase, and DNase I at 37°C for 25 minutes. The digested tissue was then passed through a 70-μm mesh filter to obtain a single-cell suspension, which was subsequently diluted in PBS containing 10% FBS. The cDNA library was prepared by Chromium Next GEM sngle cell 3' kit v3.1 for Dual index following manufacturer's instructions.