GSM8545864: MIRI+Compound C1; Mus musculus; ncRNA-Seq
1) DNaseI was used to digest the DNA fragments present in the total RNA sample and purify and recover them; 2) Ribosomal RNA removal from total RNA using the Ribo-off method; 3) Prepare the RNaseR reaction system, digest the linear RNA components, and purify the recovery reaction products; 4) RNA fragmentation at a certain temperature and ion environment; 5) Synthesis of one-strand cDNA using fragmented RNA as template; 6) synthesis of cDNA bistrands, in which dUTP is used instead of dTTP to synthesize bistranded cDNA; 7) Double-stranded cDNA is repaired at the end, with a "A" base added to the 3' end and linkers; 8) UDG enzyme digestion of U-stranded cDNA containing U-strand, and PCR amplification at the same time; 9) The quality of the constructed library was tested, the library products that passed the quality inspection were circularized, and the circular DNA molecules were replicated through rolling rings to form DNA nanospheres (DNBs);