HiFi Viral PacBio sequencing of SARS-CoV-2 vRNA isolated from tissues of an autopsy case
The PacBio HiFi Viral protocol was used to generate long-read libraries from SARS-CoV-2 vRNA. In short, vRNA was enriched and used as input into molecular inversion PCR to generate tiled amplicons across the genome. Amplicons were barcoded, multiplexed, and used as input for the SMRTbell template preparation.