16S metabarcoding of soil sample
We used DNA metabarcoding to evaluate successional changes in soil bacteria and fungi, comparing land managed for cattle, young, and older secondary forests at moist and dry sites in the Republic of Panama.  The primer pair 515F and 806R (Caporaso et al. 2012), modified to include Illumina adaptors on both primers and a 10 bp index on the reverse primer, was used to amplify the V4 16S region.  Products were cleaned an normalized with SequelPrep plates, pooled, and the resulting library was concentrated using KAPAPure beads