GSM9735915: SKNAS Day 3 CTRL_A; Homo sapiens; RNA-Seq
Total RNA was isolated from mouse tumor samples and subjected to whole transcriptomic profiling. RNA extraction was done suing the RNeasy Mini Kit, following the manufacturer's direction. RNA quantity and quality were determined using the Agilent 4200 TapeStation system at the Genomics Core of the Center for Cancer Research at NIH. RNA samples with an RNA integrity score higher than 9 were proceeded for library construction using the TruSeq Stranded Library Preparation Kit and sequencing on a NovaSeq X Plus sequencer at the Sequencing Core Facility at Federick National Laboratory for Cancer Research.