GSM8859023: Stim 4-1BB agonistic ab, Bleo Lung Tregs, Bulk RNA-seq, pool 4; Mus musculus; RNA-Seq
Cells were lysed with Trizol Reagent, and RNA was extracted using a Qiagen kit PolyA pulldown of RNA, RNA amplification using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara), cDNA library synthesis with NexteraXT Kit