GSM8757727: Control, rep21; Homo sapiens; RNA-Seq
Total RNA was isolated from PBMCs using RNAiso Plus (Takara, Japan). mRNA was purified with poly T oligo-attached magnetic beads to establish cDNA libraries for RNA sequencing. Library fragments (370–420 bp) were purified using the AMPure XP System (Beckman Coulter, Beverly, USA). The cDNA library quality was assessed using the Agilent Bioanalyzer 2100 system. Paired-end sequencing (150 bp) was performed on an Illumina NovaSeq platform. Transcriptomic mapping and profiling were conducted by Novogene (Beijing, China).