WGS of Schizosaccharomyces pombe: wild type
A total amount of 0.2 g DNA per sample was used as input material for the DNA library preparations. Briefly, genomic DNA sample was fragmented by Covaris LE220R-plus (Covaris, USA) to a size of 350 bp. Then DNA fragments were endpolished, A-tailed, and ligated with the full-length adapter for Illumina sequencing, followed by further PCR amplification. PCR products were purified by AMPure XPsystem (Beckman Coulter, Beverly, USA). Subsequently, library quality was assessedon the Agilent 5400 system (AATI) and quantified by real-time PCR (1.5 nM). The qualified libraries were pooled and sequenced on Illumina platforms with PE150 strategy in Novogene Bioinformatics Technology Co., Ltd (Beijing,China), according to effective library concentration and the data amount required.