GSM8717769: mouse miRNA-seq Brain WT 1655; Mus musculus; miRNA-Seq
Tissues were extracted and frozen on dry ice. A bead mill (Retsch, Düsseldorf, Germany) was used to pulverize the tissue. RNA was subsequently extracted from the tissue powder using PeqGold TRIFAST, a phenol-based extraction protocol, (VWR, Germany) according to the manufacturer's protocol. RNA integrity was visualized using a Bioanalyzer (Agilent, Santa Clara, CA, USA) Each sample was prepared for library construction separately. All 30 libraries were prepared using the NEXTflex small RNA-Seq kit v3 (BIOO Scientific, Austin, TX, USA) according to the manufacturer's recommendations