16S rRNA amplicon sequencing of mouse feces: Cohoused_naproxen_C57B6J.COX.DKO_Female_Day0_5
DNA was extracted from samples using the DNEasy PowerSoil Pro Kit (Qiagen) according to the manufacturer's protocol. Barcoded PCR primers annealing to the V1-V2 region of the 16S rRNA gene were used for library generation. The resulting library was sequenced on the Illumina MiSeq using 2x250 bp chemistry.