GSM9257869: HepG2-hCAR cells, con-3, 72 hours; Homo sapiens; RNA-Seq
RNA samples were isolated using the miRNeasy Mini Kit (Qiagen) following the manufacturer's instructions. Total RNA was quantified using NanoDrop ND-1000 instrument. About 1 to 2 μg of total RNA was used to prepare the sequencing library. In brief, total RNA is enriched by oligo (dT) magnetic beads (rRNA removed); RNA-Seq library was prepared by using KAPA Stranded RNA-Seq Library Prep Kit (Illumina), which incorporates dUTP into the second cDNA strand and renders the RNA-Seq library strand specific. The completed libraries were qualified with Agilent 2100 Bioanalyzer and quantified by absolute quantification quantitative PCR method.