WGS of Klebsiella pneumoniae: Kp10
Bacterial DNA was extracted using the QIAsymphony DSP Virus/Pathogen Kit (Qiagen, Hilden, Germany). Genomic libraries were prepared with the Illumina DNA Prep Kit and Nextera DNA CD Indexes (Illumina, San Diego, CA, USA). Quantification was performed using a Qubit Flex Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA), and library quality was assessed with the Agilent 4150 TapeStation System and High Sensitivity DNA ScreenTape (Agilent, Santa Clara, CA, USA). Sequencing was carried out on the Illumina MiSeq platform with MiSeq Reagent Kits v3 (600 cycles) using the 2  150 bp paired-end protocol