GSM9463425: CRISPRko; D11+IFNg, Rep1; Homo sapiens; OTHER
Target cells were harvested and genomic DNA extracted using NucleoSpin Blood XL kit (Clontech). Libraries were generated using one-step PCR to amplify the integrated sequence within the construct and the addition of sample barcodes. PCR products were purified using AMPure beads and samples sequenced using Novaseq6000.