GSM9347159: H55.1C wild-type cells exposed to sorafenib 12h; Mus musculus; RNA-Seq
Total RNA was extracted from frozen liver cells or tissues using a Maxwell RSC instrument (Promega Italia, Milan) and a Maxwell RSC miRNA from a tissue purification kit (#AS1460, Promega) according to the manufacturer's instructions. RNA quality and integrity were checked by a Bioanalyzer 2100 and Agilent RNA 6000 Nano Kit (No. 5067-1511 Agilent, Santa Clara, CA, USA). RNA-seq libraries were prepared using the Qiagen QIAseq Fast Select rRNA HMR kit (no. 334386, Qiagen Düsseldorf, Germany) for ribosomal RNA depletion and the Qiagen QIAseq stranded total RNA library kit (no. 180745, Qiagen) according to the manufacturer's instructions. RNA sequencing was performed according to the Illumina pipeline on a NextSeq 500 instrument (Illumina, San Diego, CA, USA.) using the NextSeq 500/550 High Output Kit v2.5 150 Cycles (#20024907, Illumina).