GSM9499970: 2_CMO_Dura_EC-IM; Mus musculus; OTHER
Samples were labeled with CMO1-12 and these samples were pooled for each tissue. Cells were FAC-sorted into 1.5 ml collection tubes containing 25 µl Sort Buffer (FACS Buffer supplemented with 1:200 RNAase inhibitor, New England Biolabs, M0314). Up to 20,000 sorted cells per reaction were loaded on a Chromium controller (10x Genomics). Complementary DNA amplification and library preparation were performed according to the user guide for the Chromium Next GEM Single Cell 3′Reagent kits v.3.1 (CG000388 RevC). The libraries were sequenced on a NextSeq 1000/2000 machine (Illumina) with a sequencing depth appropriate to reach 20,000 reads per cell.