GSM9821993: HR5_GEX_411; Mus musculus; RNA-Seq
Mice Treatment : 100 uL of affinity-purified, filter-sterilized recombinant HRS (3-5 mg/mL) was administered via bilateral, intramuscular hamstring injection (50 ul/side) of 8-10 week old female C57BL/6 mice to induce myositis; control mice received PBS only. Mice were sacrificed 2 weeks or 6 weeks post-immunization for collection of muscle tissue. Physical and enzymatic digestion of groups of 2-3 pooled muscle samples yielded single cell preparations that were then subjected to scRNAseq using the 10X Genomics platform. Experimental procedures followed established techniques using the Chromium Single Cell 5' Library V1 kit +V(D)J Enrichment Kit, Mouse T Cell (10x Genomics).