GSM9256676: CRD002, baseline visit; Homo sapiens; RNA-Seq
Platelets were isolated using CD45 and TER119-targeted microbeads to deplete leukocytes and red blood cells and lysed with Qiazol lysis reagent followed by platelet RNA isolation with Direct-zol RNA microspin columns (Zymo Research). A total of 1000 ng RNA was used for the construction of sequencing libraries. Sequencing libraries were prepared using the Clontech SMART-Seq HT with Nxt HT kit.