GSM8941470: Kpy_1; Homo sapiens; OTHER
CUT&Tag experiments were performed following the instructions of the NovoNGS® CUT&Tag 4.0 High-Sensitivity Kit (for Illumina®) (N259-YH01, Novoprotein). A total of 100,000 cells were immobilized on concanavalin A (ConA)-coated magnetic beads, followed by permeabilization using digitonin. Each sample was incubated overnight at 4°C with 1 μg of either anti-pan Kpy or anti-pan Kla antibodies. Subsequent steps included secondary antibody incubation, transposome integration, and fragmentation reactions. The reaction products were subjected to DNA extraction, PCR amplification, and purification to construct next-generation sequencing libraries. Libraries were sequenced on a HiSeq X Ten platform (Illumina) with 150-bp paired-end reads, following the manufacturer's instructions for sequencing read length and dual indexing (Annoroad).