GSM9207680: mESC, EED rescue 24h, JARID2KO background, H3K27me3 ChIP; Mus musculus; ChIP-Seq
Cells were fixed with 1% Formaldehyde. After qenching and cell lysis, chromatin was sonicated using Diagenode bioruptor. Libraries for ChIP-seq were prepared according to manufacturer's instructions (Illumina). Briefly, IP'ed DNA (∼5 ng) was end-repaired using End-It Repair Kit (Epicenter), tailed with deoxyadenine using Klenow exo− (New England Biolabs), and ligated to custom adapters with T4 Rapid DNA Ligase (Enzymatics). Fragments of 200-400bp were size selected using Agencourt AMPure XP beads, and subjected to PCR amplification using Q5 DNA polymerase (New England Biolabs).