GSM8993148: Splenic_cDC1_Notch2cKO_R3; Mus musculus; RNA-Seq
Total RNA was isolated from the indicated sort-purified cell subsets using the Qiagen RNeasy Mini Kit according to the manufacturers instructions and including the optional DNase digestion. RNA quality was assessed using a Fragment Analyzer. RNA libraries for RNA-seq were prepared using TruSeq Stranded Total RNA Library Prep Kit (Illumina) following manufacturer's protocols. Samples were run on an Illumina NextSeq 2000 configured for 55 paired-end reads.