GSM9535674: Plastic_Day5_techRep4_bioRep1; Homo sapiens; RNA-Seq
Cells were lysed in QIAzol reagent and RNA was extracted using the Direct-zol RNA Microprep kit (Zymo Research). RNA integrity was assessed on an Agilent TapeStation (RNA ScreenTape assay, Software A.02.01 SR1), and samples with RNA integrity number (RIN) ≥ 8 were used for downstream analyses. Libraries were prepared using the Illumina mRNA library preparation kit with poly-A enrichment from the recommended Novogene RNA input. Sequencing was performed on an Illumina platform using paired-end 150 bp reads, generating approximately 9 Gb of data per sample (~30 million reads per sample).