GSM9256642: Brainstem_infected_rep3; Mus musculus; RNA-Seq
TG and brainstem were harvested from infected (n=21) and mock (n=10) mice. Tissues were either immediately immerged in RNAlater (Fischer Scientific) and stored at -80°C until use or directly stored at -80°C until use.  RNA was extracted using the NuceloSpin RNA kit (Macherey-Nagel) according to the Midi protocol in the manufacturer's instructions. RNA quality was confirmed using a Bioanalyzer RNA Analysis kit (Agilent). RNA sequencing libraries were prepared from 250 ng total RNA using the TruSeq Stranded mRNA Library Prep kit (Illumina)