GSM9206224: QKI_MYC_MUT_IP1; Homo sapiens; RIP-Seq
For each 10cm plate (~15 million cells), cells were washed once with cold phosphate buffered saline, and then UV crosslinked (254 nm, 400 mJ/cm2) on ice. Cells were then spun down, supernatant removed, and washed with cold phosphate buffered saline. Cell pellets were flash frozen on dry ice and stored at -80°C. eCLIP was performed as described in Blue, SM, et al. Nature Protocols (2022), PMID 35322209. IP fragments were ligated to 3' RNA adapters and RNA was isolated and reverse-transcribed following SDS-PAGE and Western blot. cDNA products were ligated to 5' adapters and PCR-amplified.