RNA_MSIE_BT1
Total RNA was extracted from sorted T cells using TRIzol reagent. RNA quality and integrity were assessed using the RNA Nano 6000 Assay Kit on a Bioanalyzer 2100 system (Agilent). Sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit (Illumina). Poly-A mRNA was isolated, fragmented, and reverse transcribed to cDNA. Libraries were size-selected (150-200 bp), amplified, and sequenced on an Illumina NovaSeq 6000 platform to generate paired-end reads.