WGS of Australyichthys aegis: muscle
Archival genomic DNA showed signs of degradation and therefore was enzymatically treated to repair a variety of types of damage, following the manufacturers protocol (NEBNext FFPE DNA Repair Mix, M6630S). DNA was sheared mechanically using sonication in a Covaris M220 focused ultra-sonicator, targeting a size range of 550 bp. A sequencing library was made from 50 ng of repaired, sheared DNA using the Watchmaker DNA Library Prep Kit (Watchmaker Genomics, 7K0103-096) with 5 cycles of PCR amplification. The resulting library was size selected using paramagnetic beads (Omega Bio-Tek) to remove fragments under 275 bp.