Raw reads: Tsu-0_met1_03 DNA
The plant material from rosettes of a single 35-days-old individual was finely ground with mortar and pestle in liquid nitrogen, and high-molecular-weight (HMW) DNA extraction was carried out as published (Rabanal et al., 2022; doi: 10.1093/nar/gkac1115). HMW-DNA (120 ng/Î¼l) was sheared with Covaris gTUBEs (PN 520079) in a Eppendorf Centrifuge 5424 at 4,800 rpm (soft) for 3 x 1 min. Different amounts of input DNA (1480 - 5000 ng) were used for preparation of individual Tsu-0 met1 mutant HiFi SMRTbell libraries with SMRTbell Express Template Prep Kit 3.0 with 10 adapters bc1001-bc1003 and bc1008-bc1016. Prior to sequencing the final pooled libraries were size-selected on a BluePippin (SageScience) with 10 kb cutoff in a 0.75% DF Marker S1 High Pass 6-10 kb v3 gel cassette (Biozym). Final libraries were sequenced on a single SMRTcell, and resequenced on another SMRTcell multiplexed with other libraries, with the Sequel II system (Pacific Biosciences) using the Binding Kit 2.2.