GSM8545832: C17,TACL-ON,rep1; Homo sapiens; ATAC-seq
ATAC-seq was conducted following the Omni-ATAC protocol. In summary, 200,000 cells were lysed using a solution containing 0.1% NP-40, 0.1% Tween-20, and 0.01% digitonin, then incubated with a homemade Tagment DNA Enzyme for 30 minutes at 37 °C. DNA purification was carried out using the QIAGEN MinElute Reaction Cleanup Kit. Library fragments were amplified with Phusion High-Fidelity PCR Master Mix with HF Buffer (Thermo Fisher Scientific, catalog no. F531S) and custom primers featuring unique single or dual indexes. Purification of the libraries was performed using AMPure XP beads (Beckman Coulter, catalog no. A63881), following the manufacturer's guidelines. The quality of the constructed libraries was assessed using the Agilent Bioanalyzer 2100 with the DNA 7500 kit (catalog no. 5067-1504).