GSM9347015: delta clpB, 30C, 1; Caulobacter vibrioides NA1000; RNA-Seq
Cells were pelletted at 8000g for 2 minutes, and pellets snap-frozen in liquid nitrogen. Frozen pellets sent to SeqCenter, LLC for RNA isolation, library preparation, and sequencing. Samples were DNAse treated with Invitrogen DNAse (RNAse free). Library preparation was performed using Illumina's Stranded TotalRNA Prep Ligation with Ribo-Zero Plus kit with custom primers for depleting Caulobacter rRNA. Sequencing was done on a NovaSeq X Plus, producing paired end 150bp reads.