Genomic Discovery of EF-24 Targets Unveils Antitumorigenic Mechanisms in Leukemia Cells
Automated RNA-seq NGS library preparation was performed on the Eppendorf epMotion 5075 Liquid Handler using the Illumina Stranded mRNA Prep, Ligation kit. Prepared NGS libraries were assessed by quantitative analysis using the Invitrogen Qubit dsDNA High Sensitivity Assay Kit and qualitative analysis using the Agilent 4200 TapeStation and D5000 ScreenTape System. Libraries were loaded on an Illumina P3 200-cycle Reagent kit and sequenced on the NextSeq 2000 platform.