metagenome of panax notogensing soil treated by biochar and quicklime
At green ripe stage of three-year-old P. notoginseng, soil samples were collected from six randomly selected locations within each plot and pooled into a composite sample. Three biological replicates were used for microbial community analysis. Total genomic DNA was extracted using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) following the manufacturers protocol. The bacterial 16S rRNA gene V3V4 region was amplified using primers 338F (5-ACTCCTACGGGAGGCAGCAG-3) and 806R (5-GGACTACHVGGGTWTCTAAT-3). The fungal ITS region was amplified using primers ITS1F (5-CTTGGTCATTTAGAGGAAGTAA-3) and ITS2R (5-GCTGCGTTCTTCATCGATGC-3). Sequencing libraries were constructed and subjected to paired-end sequencing on the Illumina NovaSeq 6000 platform (Illumina, USA).