GSM9820974: LNCaP Input (Whole media); Homo sapiens; ChIP-Seq
Following fixation and quenching, we performed chromatin immunoprecipitation, size selection, and purification using the Diagenode iDeal ChIP kit for Transcription Factors (Diagenode, Cat: C01010055) according to manufacturer's protocol. Library preparation and sequencing was performed at the University of Illinois Urbana-Champaign (UIUC) Roy J. Carver Biotechnology Center using the Ovation® Ultralow System V2 DNA-Seq Library Preparation Kit (Tecan, Cat: 402666). The libraries were pooled; quantitated by qPCR and sequenced on one 25B lane for 151 cycles from both ends of the fragments on a NovaSeq X Plus with V1.0 sequencing kits (reads were 150bp in length). Fastq files were generated and demultiplexed with the bcl-convert v4.1.7 Conversion Software (Illumina).