GSM8508140: polII_ChIP-seq_0h_Input-1; Mus musculus; ChIP-Seq
Cells were fixed with 3% formaldehyde for 10 minutes. After quenching and cell lysis, Chromatin was sonicated using Bioruptor plus (Diagenode). Protein-DNA complexes were isolated with antibody. Cells were crosslinked with 254 nm UV at 0.15 J/cm2. After cell lysis, RNA was digested with RNase T1. CLIP-seq protein-RNA complexes were isolated with antibody. ChIP-seq libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) following manufacture's protocols. CLIP-seq libraries were prepared using SMART-Seq® Stranded Kit according to the manufacturer's instructions.