GSM9307335: mouse colon, control, rep 1; Mus musculus; RNA-Seq
Flash frozen mouse colon samples were minced on dry ice and fixed according to 10X Genomics Tissue Fixation and Dissociation for Chromium Fixed RNA Profiling protocol. All samples were fixed for 18h. Samples were subsequently dissociated into single cells for the Fixed RNA Profiling (Flex) assay according to the 10X Genomics Chromium Fixed RNA Profiling Reagent Kits for Multiplexed Samples user guide. Library prepartation and construction was performed according to the 10X Genomics Chromium Fixed RNA Profiling (Flex) Reagent Kits for Multiplexed Samples protocol.