Oxford Nanopore whole genome sequencing of Staphylococcus warneri strain ABSKSKLAB05
Whole genome sequencing of Staphylococcus warneri strain ABSKSKLAB05 isolated from rhizosphere soil collected from agricultural fields in Telangana, India. High-molecular-weight genomic DNA was prepared for Oxford Nanopore sequencing through end-repair, dA-tailing, and native barcode ligation using the SQK-NBD114.96 kit, followed by purification with AMPure XP beads. The prepared libraries were sequenced on the PromethION platform using FLO-PRO114-M flow cells to generate long-read sequencing data. These long reads provide high genome coverage and enable accurate resolution of repetitive genomic regions, facilitating hybrid genome assembly and genome mining for biosynthetic gene clusters associated with antimicrobial and other bioactive secondary metabolites.   Authors: Anushka Bhrdwaj (Department of Bioinformatics, Alagappa University, Karaikudi - 630003, Tamil Nadu, India), Dr. Anuraj Nayarisseri (In Silico Research Laboratory, Eminent Biosciences, Indore - 452010, Madhya Pradesh, India), Prof. Sanjeev Kumar Singh (Department of Bioinformatics, Alagappa University, Karaikudi - 630003, Tamil Nadu, India)