RNA-Seq of nonlesional dermis of Bullous patient
Single-cell libraries were constructed following manufacturer's protocol using either the Chromium Next GEM Single Cell 3' Kit v.3.1 (10X Genomics) kit, targeting between 5k and 10k cells, or the Chromium Next GEM Single Cell Fixed RNA Sample Preparation Kit alongside the Chromium Fixed RNA Kit, Human Transcriptome (10X Genomics), targeting 10k cells. Libraries were sequenced on the Illumina NovaSeq X+ paired-end platform and data demultiplexing was performed using BCLConvert software v4.3.13 (Illumina). Sequenced reads in FASTQ format were aligned and analyzed via the CellRanger count pipeline v8.0.0 (10X Genomics) using the refdata-gex-GRCh38-2020-A (10X Genomics) reference database under default settings.