RNA-Seq of Pseudomonas sp.
RNA libraries were prepared using CORALL Total RNA-seq Library Prep Kit with Unique Dual Indices with RiboCop HMRv2 rRNA Depletion Kit (Lexogen). cDNA was synthesized: the 3 ends of first-strand cDNA fragments were ligated with a linker containing Illumina-compatible P5 sequences and Unique Molecular Identifiers (UMIs). During the following steps of second strand cDNA synthesis and ds cDNA amplification, i7 and i5 indices as well as complete adapter sequences required for cluster generation were added. Sequencing was carried out on NovaSeq X 10B (Illumina), 2/150 bp reads.