GSM9207729: Sorbs2 WT 6m heart rep4; Mus musculus; RNA-Seq
Ventricular myocardium was dissected, snap-frozen in liquid nitrogen, and homogenized using stainless steel beads. Total RNA was isolated using Direct-zol RNA Miniprep Plus kits (Zymo Research, R2071). RNA integrity was confirmed (RIN >9). Poly(A) RNA was enriched, fragmented, and reverse transcribed. Stranded RNA-seq libraries were prepared using the Illumina Stranded mRNA kit, and sequenced on an Illumina NovaSeq 6000 platform (150 bp paired-end).