GSM9307811: MDA-MB-231 cells, low flow rate, exp2 rep2; Homo sapiens; RNA-Seq
RNA was harvested using an in-house protocol. Cells were directly lysed using TRIzol (Qiagen), and phase separation was performed with 0.2 mL chloroform for every 1 mL of TRIzol. RNA was precipitated using 0.5 mL 100% isopropanol per 1 mL of TRIzol used for the initial homogenization. Then, the RNA precipitate went through a series of 75% ethanol and washes. Finally, RNA was resuspended in 20 microliters of RNase-free water. cDNA libraries for RNA-Seq were prepared using a KAPA Stranded mRNA-Seq kit following the manufacturer's protocols.