GSM8653435: WT_16h_flow_10/3; Homo sapiens; RNA-Seq
Media is aspirated from flow slide. Monolayer is gently washed with PBS once. Two rounds of 200ul RLT buffer (Qiagen RNAesy kit) are used to lyse and collect the cells into tubes. Samples were kept in -80C until all experiments were concluded and extracted together in mixed batches to minimize variability. mRNA-Seq libraries were constructed using NEBNext Ultra II RNA Poly(A) kit (NEB #E7765L)