GSM9753124: Alveolar_Macrophage_KO_Recr-1; Mus musculus; ATAC-seq
Flow-sorted cells were washed twice with 1X PBS and collected by scraping and pelleting prior to counting. Approximately 50K cells were pelleted and processed in duplicate as described in the Omni-ATAC-seq protocol published by Corces et al (Nature Methods, 2017). Uniquely indexed libraries were pooled and sequenced on an Illumina NovaSeq 6000 using 150 bp paired-end reads at the Genomics Shared Resource at the University of Anschutz Medical Campus.