GSM9675366: HG_hepato_4; Haliotis discus hannai; RNA-Seq
Total RNA was extracted from hepatopancreas and mantle tissues collected from the selected high- and low-growth abalones. Frozen tissues were pulverized in liquid nitrogen using a pre-chilled mortar and pestle, and RNA was isolated using TRIzol reagent (Invitrogen, Waltham, MA, USA) according to the manufacturer's instructions. RNA-seq libraries were prepared from 1 μg of total RNA per sample using the TruSeq Stranded mRNA Sample Prep Kit (Illumina, San Diego, CA, USA) following the manufacturer's instructions. Briefly, mRNA was purified using oligo(dT)-attached magnetic beads, fragmented, and reverse-transcribed to generate first-strand cDNA, followed by second-strand synthesis. After adapter ligation and PCR amplification, 28 libraries were sequenced on the Illumina NovaSeq platform to generate paired-end reads of 2 × 101 bp.