GSM8859976: SK-MEL-28 siDANCR cells (2); Homo sapiens; RNA-Seq
Total RNA was purified using the GeneJET RNA purification Kit (ThermoFisher Scientific). Residual genomic DNA was then removed using DNA-free™ DNA Removal Kit (ThermoFisher Scientific). polyA RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP for strand specific library.