rat 190, stress
Rat received intraperitoneal injection of vehicle (sterile distilled water in a volume of 1 ml/kg body weight). 30 min after injection, rat was submitted to acute restraint stress for 1 h (immobilization of animals combined with bright light and acoustic exposure). Rat was left undisturbed in their home cages throughout the stress procedure. Then, the animals were tested in an Elevated Plus-maze (within 5 min, at 23.5 h after injection). Rat was withdrawn from the experiment at 24 h after injection. mRNA fraction from total RNA of rat hippocampus was used for high-throughput RNA-sequencing (RNA-Seq). At least 10 million reads (1/50 nt) were generated.