GSM9820480: HepG2 ARID1B-KO RNA-seq, DMSO, rep2; Homo sapiens; RNA-Seq
1x10^6 cells pelleted (500xg, 1 min). Total RNA isolated with Monarch Total RNA Miniprep Kit (NEB T1010) with on-column DNase I digestion. KAPA mRNA HyperPrep Kit (Roche #08098123702) from 750 ng total RNA. Fragmentation 6 min 94°C. Adapter ligation 7 µM stock. 9 PCR cycles. Paired-end 75 bp on Illumina NextSeq 100.