RNA-Seg of mus musculus: adult male kidney
Total RNA were extracted separately using TRIzol reagent. RNA integrity (RIN value) was precisely measured with the Agilent 4200 TapeStation system. The cDNA libraries were construction subsequently. The libraries concentration was accurately quantified using Kapa qPCR and the library fragment size was precisely determined with the Agilent 4200 TapeStation. Different libraries were pooled according to the effective concentration and the desired amount of raw data, followed by Illumina PE153 sequencing.