GSM9207119: CD8+CD90.1+P4-6CD25high transgenic TCRTAG CD8 T cells isolated 2d post activation in LMTAG infected spleens, rep 1; Mus musculus; RNA-Seq
CD8 T cells were sorted directly into Trizol LS and frozen. Total RNA was extraced from sorted cells using the Rneasy Micro kit (Qiagen)and amplified using the SMART-Seq v4 UltraLow Input RNA Kit (Clontech). Libraries were prepared using NEBNext polyA mRNA sample kits (NEB).