GSM9349169: 6B_CnT_H3K27ac_replicate1; Mus musculus; OTHER
Cells were harvested by Accutase dissociation and used in CUT&Tag (Kaya-Okur et al. 2020, PMID: 32913232) assays for different histone mark antibodies and a non-specific IgG control (H3K27ac, H3K4me1, H3K4me3, H3K27me3, total rabbit IgG). Genomic DNA was extracted from cells after CUT&Tag using proteinase K digestion and Zymo DNA clean and concentrator clean-up. Genomic DNA isolated from each CUT&Tag cell sample replicate was subjected to end repair and 6-base-seq library construction (Fullgrabe and Gosal et al. 2023, PMIID: 36747096).