GSM9020445: dMSC, baseline, rep1; Homo sapiens; RNA-Seq
Samples were lysed at passage 3-4 in Trizol reagent (Sigma-Aldrich, Burlington, MA, USA) according to the manufacturer's instructions. The RNA sample quality was controlled using BioAnalyser and the RNaA 6000 Nano Kit (both Agilent Technologies, Santa Clara, CA, USA). PolyA RNA was purified with the Dynabeads®® mRNA Purification Kit (Ambion, Waltham, MA, USA). An Illumina library was made from polyA RNA with NEBNext® Ultra™ II RNA Library Prep (New England Biolabs, Ipswich, MA, USA), according to the manufacturer's instructions. Concentrations of nucleic acids in the obtained libraries were analyzed by the Qubit dsDNA HS Assay Kit using Qbit 2.0 equipment (Thermo Fisher Scientific, Waltham, MA, USA). The distribution of library fragment lengths was assessed using the Agilent High-Sensitivity DNA Kit (Agilent Technologies, Santa Clara, CA, USA).