GSM9853731: RAI1 WT, HET and KO cortical differentiation scRNA-seq library; Homo sapiens; RNA-Seq
Approximately 3 × 10^5 cells per sample were washed with ice-cold PBS containing 0.04% BSA and fixed using the Chromium Next GEM Cell Fixed RNA Sample Preparation Kit (10x Genomics). Cells were incubated overnight at 4°C in Fixation Buffer, quenched, treated with Enhancer, supplemented with glycerol for storage, and submitted for Chromium Fixed RNA Profiling library preparation. Single-cell RNA sequencing libraries were prepared using the Chromium Fixed RNA Profiling (Flex) platform (10x Genomics) according to the manufacturer's protocol. Libraries were sequenced on an Illumina NovaSeq 6000 platform using 28 × 91 bp paired-end sequencing.