RNA-Seq of HRECs transduced with a PRDX1-targeting shRNA lentivirus-replication 2
RNA-seq transcriptome libraries were prepared following TruSeqTM RNA sample preparation Kit from Illumina (San Diego, CA), using 1g of total RNA. Shortly, messenger RNA was isolated with polyA selection by oligo(dT) beads and fragmented using fragmentation buffer. cDNA synthesis, endrepair, A-base addition, and ligation of the Illumina-indexed adaptors were performed according to Illuminas protocol.