GSM8603242: Syn_Repeated_24hr_rep2; Mus musculus; RNA-Seq
Harvested tissues were homogenized, and total RNA was extracted using TRI Reagent (Molecular Research Center, Inc.). Total RNA was further purified with the Direct-zol RNA Kit (#R2062, Zymo Research, Irvine, CA) according to the manufacturer's protocol. RNA libraries for RNA-seq were prepared using NEBNext® Poly(A) mRNA Magnetic Isolation Module (for PolyA selection) NEBNext® Ultra™ ll Directional RNA Library Prep Kit following manufacturer's protocols.