Stereo-seq of D.pse adult male abdomen section
Abdomen segments were dissected from 5- to 7- day-old Dpse adult flies in cold PBS. Dissected samples were rinsed in PBS and dried with wipers. Samples were embedded in Tissue-Tek OCT and immediately transferred to an -80 freezer for storage. Cryosections were cut at a thickness of 10 mm in a Leika CM1950 cryostat at -20. Stereo-seq library preparation was performed and sequenced was performed.