GSM9307310: CPC;ApcPten2; Mus musculus; RNA-Seq
RNA was extracted from frozen samples using the RNeasy Mini Kit (Qiagen) TruSeq Stranded mRNA library was prepared from total RNA using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) according to the TruSeq Stranded mRNA Reference Guide (Document #1000000040498, v00). The procedure included poly(A)+ RNA purification, fragmentation, first- and second-strand cDNA synthesis, adapter ligation, and PCR amplification, selecting fragments of approximately 200–400 bp in length.