Environmental water sample D0623
The DNA samples were end-prepared using NEBNext Ultra II End Repair/dA-Tailing Module and barcode adaptor were ligated to the end - prepared DNA using Blunt/TA ligase master mix. The adaptor ligated DNA were cleaned with 1X AmPure XP beads and barcoded by PCR using LongAmp Taq 2X master mix. The equimolar concentration of barcoded sample was pooled and library was prepared by ligating sequencing adapters (SQK-LSK114) onto double-stranded DNA fragments using NEB Quick T4 DNA Ligase. After purification with 0.6X AMPure XP beads, the prepared library was ready for library quality control.