GSM9463215: HN95 leaves under saline-alkaline stress with MT treatment，the treatment，biol rep 1; Glycine max; RNA-Seq
The total RNA was extracted from 0.1g fresh samples preserved at -80℃ using the TransZol Plant (TRANS) following the manufacturer's instructions. mRNA was enriched from 1 μg of high-quality total RNA using oligo(dT) magnetic beads (Illumina). Purified mRNA was fragmented into ~200 bp fragments using divalent cations under elevated temperature. The first-strand and second-strand cDNA were synthesized sequentially, and amplified by PCR to generate final cDNA libraries.