Metagenomic sequencing of rumen metagenome: CON, untreated BSF, and heat-treated BSF diets in goats
Fragmentation: Genomic DNA was randomly sheared using a Covaris ultrasonicator. Fragment selection: Magnetic beads were used to select DNA fragments of 200-400 bp. End repair and adapter ligation: Fragment ends were repaired, an A base was added to the 3' ends, and sequencing adapters were ligated. PCR amplification: The ligation products were PCR-amplified to enrich the library. Purification and circularization: PCR products were purified and circularized to generate single-stranded circular DNA molecules. Library quality control: Library concentration was measured, and only qualified libraries were used for sequencing.