GSM9736652: HL60_Combination_D2_1; Homo sapiens; RNA-Seq
Total RNA was extracted from cultured cells. RNA concentration and purity were assessed using NanoDrop 2000, RNA integrity was evaluated by agarose gel electrophoresis, and RNA integrity number (RIN) was determined using an Agilent 2100 Bioanalyzer. mRNA was enriched using oligo(dT)-attached magnetic beads and fragmented into short fragments. First-strand cDNA was synthesized using random primers, followed by second-strand cDNA synthesis. After end repair, A-tailing, and adaptor ligation, the libraries were amplified by PCR and purified. Libraries were constructed using the Illumina TruSeq RNA Sample Preparation Kit and sequenced on the Illumina platform with paired-end 150 bp reads.