Searching for Molecular Markers in the Prefrontal Cortex and Hippocampus of Rat Offspring Following Exposure to Maternal Western Diet
RNA was extracted in accordance with the manufacturers guidelines and subsequently subjected to additional purification using the RNA Mini Kit (A&A Biotechnology, Poland). The total RNA concentration was measured using an ND-1000 Spectrometer (NanoDrop Technologies Inc., USA). RNA sequencing was performed as an external service by Novogene using NovaSeq X Plus Series (PE150) (6.00 G raw data per sample).