GSM9534923: p23259_Gerardo_30RNASeq-s003_Z15339-RNA-Seq-05-30-23; Macaca nemestrina; RNA-Seq
Two million PBMCs were lysed with 350 µL of Buffer RLT and then extracted by using the RNeasy Mini kit (Qiagen, Germantown, MD, USA) with on-column DNase digestion. RNA quality was assessed by using a TapeStation 4200 (Agilent, Alpharetta, GA, USA) and then ten nanograms of total RNA were used as input for cDNA synthesis by using the SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio, San Jose, CA, USA) according to the manufacturer's instructions. Amplified cDNA was fragmented and appended with dual-indexed barcodes by using the Nextera XT DNA Library Preparation kit (Illumina, San Diego, CA,USA). Libraries were validated by capillary electrophoresis on a TapeStation 4200, pooled at equimolar concentrations, and sequenced with PE100 reads on an Illumina NovaSeq 6000, yielding ~23 million reads per sample on average.