GSM8993000: WT_largeRNA_rep1; Mus musculus; Bisulfite-Seq
Total RNA from testes were extracted using TRIZol.  mRNA can be captured through beads conjugated with oligo-dT. Large or small RNA was extracted using RNA Clean & Concentrator-5 (Zymo). 100ng-1μg RNA was treated with UBS-2 buffer. For mRNA UBS-seq library preparation, the Stranded mRNA-seq Lib Prep Module for Illumina (ABclonal) was used. For large RNA UBS-seq library preparation, the converted RNA were firstly fragmented in the presence of MgCl2 and VAHTS Small RNA Library Prep Kit for Illumina V2 (Vazyme) was used. For small RNA UBS-seq library preparation, VAHTS Small RNA Library Prep Kit for Illumina V2 (Vazyme) was used.