WGS-Seq of fish skin microbiomes: Siganus canaliculatus
Fragments of the desired size were subject to end-repair, 3 adenylation, adapter ligation, and PCR amplification. The double-stranded PCR products were purified with magnetic beads, denatured by heating, and circularized with a splint oligo sequence. The resulting single-strand circle DNA (ssCir DNA) was further formatted to form the final library.