Nanopore DNA-Seq of Galaxias maculatus: adult muscle tissue
500 ng of DNA was used as the template for LSK114 library preparation kit followed by sequencing on the Nanopore MinION flowcell R10.4.1. Multiple nuclease washes were performed to increase yield due to persistent blocking of the pore by the host gDNA. Basecalling used dorado v0.7.0 super accuracy mode (dna_r10.4.1_e8.2_400bps_sup@v5.0.0)