GSM8508029: HT, Young3, 2M; Mus musculus; Bisulfite-Seq
Hypothalamus, hippocampus and OB were dissected from 2 and 12 months of age (n=3 mice per group) in manner that it maintains the anatomic separation from the neighboring brain region. DNA was extracted from these tissues using a Qiagen kit. The extracted DNA samples were sheared into fragments of 150-200 base pairs using sonication, and the fragment sizes were verified using the Agilent TapeStation with D1000 ScreenTape. The fragmented DNA underwent library preparation using the SureSelectXT Methyl-Seq Library Prep Kit, following the manufacturer's instructions. Bisulfite-Seq (Bisulfite oligonucleotide-captured sequencing)