Cecal fluid 28day-old chicken_Control_24hr_Protocatechuic.acid treated_Exp1
Cecum was dissected from euthanized chicken. Cecal fluid was collected and treated with either gallic, protocatechuic, or vanillic acid for 24 or 48hr, or left untreated. DNA was extracted at the determined timepoint with QIAamp Fast DNA Stool Kit. Nexter DNA Library Preparation Kit was used to amplify the v3-v4 regions and Nextera Index Kit to generate library. Library was quantified and sequenced using the Illumina MiSeq v3 600 kit on the Illumina MiSeq system.