RNA Seq of Xag wt in PCD non-inducing medium
Total RNA extraction was performed using TriReagent (Sigma, USA), as per the manufacturers protocol. The RNA quality was assessed using formaldehyde 1% agarose gel, and RNA integrity number. The library was prepared and Illumina-HiSeq2500 Rapid mode was utilised for sequencing by synthesis, generating around 20 million paired end reads, with minimum 150 bp read length per sample. At least 90% of reads in all the samples had QC value>30.