GSM9135802: HEK_293T; Homo sapiens; miRNA-Seq
For each cell line RNA was extracted according to Trizol Reagent (Ambion, #15596018) manufacturer instructions. The quantity and of the RNA samples were checked using nanodrop Qubit HS RNA (Invitrogen, #Q32852) and Bioanalyser small RNA assay (Agilent, #5067-1548). Libraries were generated using 1 ug of the total RNA via QIAseq miRNA kit (Qiagen, # 331502) with 14 cycles of amplification. Amplified barcoded libraries were then size selected using auto gel-purification Pippin prep 3% agarose (SAGE science) which targets ranges from 100-250bp. Libraries (size between 180-190bp) were then confirmed by Qubit HS DNA and Bioanalyzer HS DNA assay for size and concentration. The libraries were then pooled together in equimolar amounts and sequenced using an Illumina MiSeq Reagent Kit v3 kit in accordance with the manufacturer's guidelines