Response of Staphylococcus aureus SA0413Rev to combination of virulent bacteriophage vB_SauM-515A1 and linezolid
For the preparation of transcriptomic libraries, 250 ng of total RNA was taken as input. Selective removal of ribosomal RNA was performed using the Ribo-Zero Plus rRNA Depletion Kit (Illumina, USA), followed by library preparation employing the KAPA RNA Hyper Kit (Roche, Switzerland), according to the manufacturer protocol. Subsequent RNA purification steps involved the application of RNA Clean XP magnetic beads (Beckman Coulter, Brea, USA), with final library purification conducted using Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, USA). The size distribution and quality of the libraries were assessed using the Agilent High Sensitivity DNA kit (Agilent Technologies, USA), while the library concentration was quantified using the Quant-iT DNA Assay Kit, High Sensitivity (Thermo Fisher Scientific, USA). Finally, equimolar quantities of all libraries (12 pM) were sequenced by a high throughput run on the Illumina HiSeq