GSM8457565: Lung, Infected, timecourse (mixed samplse); Mus musculus; RNA-Seq
Lungs were removed and placed in PBS. The tissue was digested in a buffer containing DMEM/F12, DNase I, and collagenase I, then minced, incubated, and triturated to create a cell suspension. After two rounds of digestion and filtration, the cells were neutralized with DMEM/F12 + 10% FBS, pelleted, and red blood cells were lysed. The final cell suspension was counted using an automated cell counter before downstream analysis. 10x Genomics Chromium Single Cell Fixed RNA Profiling (Flex) Kit (cat# 100475), following the manufacturer's protocol