GSM9498095: Rep1_D4_High_Flow_1; Homo sapiens; RNA-Seq
On the day of collection, vascular beds were excised from microfluidic chips with a scalpel, washed 3 times with warm PBS, minced, and lysed with RLT buffer (Qiagen). The vascular bed compartments of the microfluidic chips were further rinsed with RLT buffer to dislodge remaining vascular bed fragments. Monolayer RFP-labeled BMVECs, NHLFs, and HBVPs were trypsinized, pelleted, and lysed with RLT buffer. RNA extraction was performed using RNeasy (Qiagen). Samples were processed at the Network Biology Collaborative Centre (NBCC). After TruSeq mRNA library prep (Illumina), Fragment Analyzer (Agilent) assessment showed all RNA quality numbers to be greater than 9.