PacBio seq of S. cerevisiae: Kl-URA3 at HMR locus
Gene conversion was induced at MAT in a strain carrying HMR::Kl-URA3 and Sc-ura3-52* and Ura- mutants were collected and pooled.  The library was prepared by growing 5 cultures starting from single colonies in YP-lactate overnight and plating on 5-FOA+galactose plates.  These were incubated at 30C until Ura- colonies appeared.  33,000 colonies were collected. Genomic DNA was prepared and was used as a template for PCR amplifying the MAT locus.  These PCR products were diluted and subsequently used as templates for PCR amplification using primers specific for Kl-ura3 that were tagged with barcodes.