GSM8757704: A549 shMock SUS rep2; Homo sapiens; RNA-Seq
Total RNA was isolated from cells using the RNAiso Plus reagent (#9109; TaKaRa, Tokyo, Japan) according to the manufacturer's instructions. Total RNA was extracted from A549 shMock and shDUSP23 tumor cluster cells. RNA quality was assessed using a bioanalyzer (RNA 600 Nano kit; Agilent Technologies, Santa Clara, CA, USA). Library construction was performed using an mRNA-seq Library Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer's instructions. High-throughput sequencing was performed using the NovaSeq 6000 platform (Illumina).