GSM9567287: D140 Retinoshperes, SCR264, Control; Homo sapiens; RNA-Seq
Briefly, RS (10 per conditions) were digested with Papain (Worthington) for 15-30 mins at 37°C on a nutator. Ovomucoid (Worthington) was added to stop the reaction. Dissociated cells were then centrifuged at 300g for 5 mins at 4°C. For each condition, cells were resuspended to a concentration of 1000 cells per ul. Libraries constructions were carried out using the Chromium Next GEM single Cell 3' Reagent kits v3.1 (Dual Index), following the manufacturer's instructions.