16S rRNA gene sequencing of soil DNA: sample 2062-20191214
Libraries for sequencing were prepared according to Illuminas 16S Metagenomic Sequencing Library Preparation guide, with the exception of using Qiagen HotStarTaq Plus polymerase for the first PCR (amplicon PCR). The template specific primers were (without the overhang adapter sequence): 515F-Y (5- GTGYCAGCMGCCGCGGTAA-3) and 926R (5- CCGYCAATTYMTTTRAGTTT-3).