GSM9347045: R19C05lineages_12hrAPF_-p35_rep2; Drosophila melanogaster; RNA-Seq
Cells were FACS-sorted into a PBS+1% BSA cushion. Single cell suspensions were subjected to counting and viability checks on the LUNA Fx7 Automated Cell Counter (Logos Biosystems) and diluted to a concentration of 700-1000 cells/ul. Single cell libraries were generated using the 10x Genomics Chromium Controller with 3' Gene Expression reagents following the manufacturer's protocol (10x Genomics). Final library quality was assessed using the LabChip GX (PerkinElmer). Libraries were subjected to paired-end sequencing according to the manufacturer's protocol (Illumina NovaSeqXPlus).