16s V3-V4  sequencing of wine sample
The amplicons from each sample were purified with Ampure beads to remove unused primers and an additional8 cycles of PCR was performed using Illumina barcoded adapters to prepare the sequencing libraries. Librarieswere purified using Ampure beads and quantitated using Qubit dsDNA High Sensitivity assay kit. Sequencing wasperformed using Illumina Miseq with 2x300PE v3-v4 sequencing kit.