GSM8683167: Hela, siDNMT1_1, rna-seq; Homo sapiens; RNA-Seq
Total RNA was extracted from each sample using the RNA mini kit (Qiagen, Germany), with quality assessed by gel electrophoresis and a Qubit. 1 μg of RNA was utilized to construct libraries. 1 μg of RNA was utilized to construct libraries with the VAHTS Total RNA-seq (H/M/R) Library Prep Kit for Illumina®. Libraries were sequenced as 151-bp paired-end reads on the Illumina NovaSeq 6000 by Genergy Biotechnology Co. Ltd. (Shanghai, China), following the manufacturer's guidelines