GSM8757893: ExpLBM_rep1; Homo sapiens; ATAC-seq
ATAC-seq was conducted following a previously described method 39. In brief, 4000 cells were collected Cells were lysed using lysis buffer containing 10 mM Tris-HCl, 10 mM NaCl, 3 mM MgCl2, and 0.1% IGEPAL CA-630. The Tn5 transposase reaction using Illumina Tagment DNA Enzyme and Buffer (Illumina) was performed at 37°C for 30 min. The reacted DNA was purified using a MinElute PCR Purification Kit (QIAGEN) and amplified for 8–15 cycles using a Nextera Index Kit (Illumina) to produce libraries for sequencing.