GSM9498557: scRNA-seq of ISKNV-infected Siniperca chuatsi; Siniperca chuatsi; RNA-Seq
At 7 days post-infection (dpi), fish were euthanized using MS-222. Spleen tissues were harvested, washed twice with sterile PBS, and stored in tissue preservation solution. To minimize individual variation, spleen tissues from six individuals per group were pooled into a single sample after dissociation. Single-cell libraries were constructed following the manufacturer's instructions (10x Genomics).