GSM9258561: tRNA-only unstressed E. coli replicate 1; Escherichia coli str. K-12 substr. MG1655; ncRNA-Seq
RNA was isolated using the PCI protocol (Sambrook & Russell, 2006) with some modifications (Petrov et al., 2022). mRNAe samples were depleted of tRNA/rRNA, then prepared with an Oxford Nanopore Technologies SQK-RNA004 library preparation kit. Nano-tRNAseq samples were prepared with a commercial Nano-tRNAseq library preparation kit (Immagina Biotechnology, Trento, Italy).