RNA-Seq of Parapseudocyclopinodes neglecta
RNA was extracted using an RNeasy micro kit following the manufacturers protocol. Because of the small size and limited specimens of A. interrupta and P. neglecta, RNA extractions were amplified using aTakara SMART-Seq HT kit following standard protocols and amplified using 17 PCR cycles.RNA quantity was checked using a Qbit fluorometer. Sequencing libraries were preparedusing Illumina DNA Prep in combination with a Nextera XT kit and the samples weresequenced on an Illumina NextSeq with 150 bp paired end (PE) reads totaling 41 million PEreads for A. interrupta and 68 million PE reads for P. neglecta