GSM9531361: HFPF-treated HUVEC biological replicate 3; Homo sapiens; RNA-Seq
Total RNA was extracted from HUVEC samples using a commercial RNA isolation kit according to the manufacturer's instructions. RNA integrity and concentration were assessed prior to library preparation. Only high-quality RNA samples were used for sequencing. RNA libraries were constructed using standard Illumina-compatible library preparation protocols. Briefly, mRNA was enriched from total RNA, fragmented, reverse transcribed into cDNA, end-repaired, adapter-ligated, and PCR amplified. Libraries were sequenced using Illumina paired-end sequencing technology.