GSM8507080: RQ4, WT, pRQ4, APH, replicate 1; Homo sapiens; ChIP-Seq
For ChipSeq, DNA fragments were enriched using antibodies against proteins of interest, DNA was purified and processed as described in Thakur et al, NAR 2022. For NS-seq DNA from cell cultures was isolated, size fractionated and subject to exunuclease digestion as described in Aladjem et al., Science 281, 1005, 1998. and Wang et al., Molecular and Cellular Biology 24:3373-86, 2004. Samples were eluted, and then purified by Monarch PCR & DNA Cleanup Kit (NEBT1030S). 100 ng of samples were used to generate the library. The kits used for the library were NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7805S) and NEBNext Multiplex Oligos for Il-lumina (Index Primers Set 1 and Set 2) (NEB, E7335S and E7500S).