GSM9534912: Empty vector (EV) transformed Medicago truncatula (A17) roots, biological replicate 1; Medicago truncatula; RNA-Seq
RNA integrity was assessed using the Bioanalyzer 2100 system (Agilent Technologies). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. Following fragmentation, first-strand cDNA was synthesized using random hexamer primers. The resulting cDNA underwent end repair, A-tailing, adapter ligation, size selection, amplification, and purification to generate the final libraries. Library quality was validated using Qubit and real-time PCR for quantification, while size distribution was checked using a Bioanalyzer. Libraries were sequenced on an Illumina platform using a "Sequencing by Synthesis" approach. More than 40 million reads were sequenced for each sample.