GSM9348154: MP2; Homo sapiens; RNA-Seq
Total RNA was extracted from the shear stress treated endothelial cells using RNAsimple Total RNA Kit (DP419,TIANGEN BIOTECH) according to the manufacturer's protocol. RNA quantification and purity were determined using Nanodrop One (Thermo Fisher Scientific). Qualified RNA samples were sent to BGI-Shenzhen (Shenzhen, China) for strand-specific library construction using the NEBNext Ultra II RNA Library Prep Kit (NEB #E7770),