Soil metagenome of Biosphere 2 Tropical Rain Forest sugar palm plots
Soil RNA and DNA were extracted sequentially using a Qiagen PowerSoil Total RNA kit, including phenol-chloroform separation, followed by a DNA Elution kit (QIAGEN Sciences Inc., Germantown, MD, USA). After library preparation and ribodepletion (Arizona State University Genomics Facility, Tempe, AZ, USA), DNA and RNA were sequenced using Illumina NovaSeq (2x150 S4 NovaSeq Flow Cell; University of Colorado Gates Institute, Aurora, CO, USA).