RNAseq of Mustela putorius furo: Adult female brain, atypical disease
RNA extracted from the brains of eight ferrets from the second challenge experiment were subjected to RNA sequencing. An aliquot of diluted ERCC ExFold RNA Spike-Mix 1 was added to the RNA, per manufacturer's guidelines, and the RNA was DNase-treated using ezDNase enzyme. RNA-seq libraries were prepared from 100, 500, or 1000 ng of the DNase-treated RNA using the TruSeq Stranded mRNA Library Prep Kit following a slightly modified manufacturers protocol, where Superscript II was replaced with Superscript III reverse transcriptase to generate cDNA. Libraries were sequenced on a NextSeq 2000 using the NextSeq 2000 P3 Reagent Kit (100-cycle) for 1 x 100 bp reads. The reads were aligned to the ENSEMBL ferret genome using HiSAT.