acRIP-seq MC38 KO-2 (CRX1466609)
Remove rRNA from total RNA using Ribo zero kit (Illumina), and then perform ac4C-IP reaction using GenSeq's RNA acetylation kit. Using NEBNext Construct an RNA sequencing library using the Ultra II Direct RNA Library Prep Kit kit. The constructed library was subjected to quality control using an Agilent 2100 bioanalyzer and tested using an Illumina HiSeq 4000 sequencer