GSM9020701: ST_mild 6OHDA_11; Mus musculus; OTHER
fresh frozen tissue The tissue sections mounted on Visium slides were fixed in pre-chilled methanol at -20°C for 30 minutes, in accordance with the 10X Genomics protocol [CG000160 Rev B]. Following fixation, hematoxylin was applied for 5 minutes, followed by a 45-second application of eosin to enhance contrast. Brightfield images were captured using a Zeiss Axiolmager.Z2 microscope equipped with Metafer 5 software. We optimized the tissue permeabilization process specifically for the striatal region, following the 10X Genomics tissue optimization guidelines [CG000238 Rev D]. Preliminary testing identified 14 minutes as the optimal permeabilization time for this brain region.