GSM8859568: IL34LacZ/LacZ brain macrophages and vascular cells; Mus musculus; RNA-Seq
All methods are listed in the associated manuscript. The quality of the single-cell suspension was assessed using a hemocytometer under a Leica DM IL LED Fluo microscope, and cell quantity was evaluated with an automated cell counter (LUNA-FX7, Logos). Cells were loaded into the 10x Genomics Chromium platform, and library preparation was performed according to the manufacturer's instructions (Chromium Next GEM Single Cell 3ʹ Reagent kits v3.1 protocol).