GSM9754276: PBMC Pre-ICI scRNA; Homo sapiens; RNA-Seq
Cryopreserved peripheral blood mononuclear cells (PBMCs) were thawed and adjusted to a final concentration of 1,000-2,000 viable cells/µL. Approximately 17,000 cells were loaded onto the Chromium X (10x Genomics) to capture a target of 10,000 cells per sample. Libraries were prepared using the Chromium Next GEM Single Cell V(D)J Reagent Kits v1.1, following the manufacturer's instructions. The 5′ digital gene expression (DGE) libraries were sequenced on an Illumina NovaSeq X system to a target depth of 50,000 reads per cell, while full-length V(D)J segments were enriched from barcoded cDNA to enable pairing of TCRα and TCRβ chains with the corresponding transcriptome. The 5′ V(D)J libraries were sequenced on an Illumina NovaSeq X system to a depth of approximately 5,000 reads per cell.