GSM8546189: PDV cell, IgG, replicate 2; Mus musculus; OTHER
Cells were fixed with 1% formaldehyde for 1min. After quenching, cells were permeabilized and immobilized onto Concanavalin-A beads and incubated overnight with IgG or Sox2 antibody. Cut & Run libraries were prepared using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina according to the manufacture's protocol. Libraries were validated using the Fragment Analyzer.