Nanopore metagenomic sequencing of microbial community: enrichment liquid fraction replicate on polycaprolactone (VT_14)
Metagenomic DNA was extracted from enrichment culture (liquid fraction, replicate) of Setun River microbial community incubated in M9 minimal medium with PCL plastic fragments. Libraries were prepared using Oxford Nanopore ligation protocol with R10.4 chemistry and sequenced on Nanopore flow cells with subsequent basecalling and quality control.