Bulk RNAseq of CHIR and Activin A treated gastruloids, mESCs and mEpiLSCs
Bulk RNAseq of CHIR and Activin A treated gastruloids, mESCs and mEpiLSCs
RNA for bulk RNA sequencing was extracted (Qiagen RNeasy Mini Kit) from pooled mouse gastruloids according to their developmental stage: full 96-well plate if at 48h, 60h and 72h, half a 96-well plate if at 96h and one-third of a 96-well plate if at 120h or 144h. At least 3 replicate samples were obtained per condition, from several independent experiments. RNA was also extracted from 2D cultures of ESCs and EpiLSCs (T=0h) following the same procedure.  Library preparation was done by the IGC/GIMM Genomics facility in Portugal.