GSM8993118: KMT2; Mus musculus; RNA-Seq
Total RNAs were purified using Qiazol RNA Isolation Reagent (QIAGEN). Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). Fragmented mRNAs were reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies) and random hexamers. Illumina sequencing adapters were ligated to the cDNAs. Ligated fragments were then amplified for 12-15 cycles and sequenced on an Illumina NovaSeq 6000.