16S amplicon sequencing of Edhazardia aedis spores
Spore solution DNA was used as a template in PCR with 515F and 805R primers with Illumina adapters. PCR product was isolated on an agarose gel and subjected to 300 base pair paired-end sequencing on an Illumina NextSeq 2000 platform following the Illumina 16S Metagenomic Sequencing Library Preparation guide.