Tridacna crocea gonad for PacBio HiFi 4
"High molecular weight DNA was extracted from the tissue sample with Nanobind Tissue Big DNA Kit (Circulomics), and the DNA size was assessed with the Femto Pulse system (Agilent Technologies). The extracted DNA was sheared with Megaruptor 3 (Diagenode), and then purified using AMPure PB (Pacific Biosciences). Library preparation was conducted using the SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences). Size selection was carried out with the BluePippin system with High Pass Plus Gel Cassettes (Sage Science) to remove library fragments smaller than 17 kb."