GSM8507164: Wild-type, replicate2; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNeasy Mini kit (QIAGEN) and treated with DNaseI (QIAGEN). Ribosomal RNA was depleted using the NEBNext rRNA Depletion Kit v2 (NEB). Libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina following the manufacturer's instructions.