GSM9055801: MASH1; Mus musculus; RNA-Seq
Liver tissue was snap-frozen in liquid nitrogen and homogenized in lysis buffer supplemented with RNase inhibitorusing a glass dounce homogenizer. The nuclei suspension was filtered through a 70-mm cell strainer, briefly centrifuged, washed and strained again through a 40-mm cell strainer. The nuclei suspension was then FACS sorted on a BD FACS Discover S8 Cell Sorter. Sequencing libraries were prepared using the 10x Genomics Chromium Next GEM Single Cell 3' RNA Library & Gel Bead Kit (v3) using the 10x Genomics Chromium X instrument  according to the manufacturer's protocol.