Compost bacterial metagenome Day 0; Replicate 2
Genomic DNA was extracted from 0.25 g samples (Day 0 and Day 30) using the PowerLyzer PowerSoil Kit (Qiagen). The V3V4 region of the bacterial 16S rRNA gene was amplified using the 341F/806R primer pair, while the fungal ITS1 region was amplified using the ITS1F/ITS2 primer pair. Amplicons were sequenced on an Illumina NextSeq 2000 platform utilizing 2  150 bp paired-end sequencing chemistry at the Australian Genome Research Facility (AGRF, Melbourne).