Genome sequence of Aureobasidium pullulans Y2311-1
Illumina library construction utilized the NEXTFLEX Rapid DNA-Seq Kit (NOVA-5144, Illumina), with fragmented DNA undergoing end-repair, adenylation, adapter ligation, and PCR enrichment on the Illumina NovaSeq X Plus platform using the NovaSeqTM X Series 25B Reagent Kit (300 Cycle, Cat. No. 20104706, Illumina). This generated 4,063,873 pairs of 150-bp raw reads, which were quality-controlled using fastp (Version 0.20.0) via trimming low-quality bases, removing short reads, and discarding those with N bases. Specifically, bases with a quality score below 20 at the read tails were trimmed. A 10-bp sliding window was then employed; when the average quality score within the window dropped below 20, all subsequent bases starting from the window were removed. Reads that were shorter than 50 bp after undergoing these quality-trimming steps were discarded, ensuring that only high-quality sequences were retained for further analysis. Illumina reads were exclusively used for quality control of PacBio raw sequencing data and subsequent evaluation of genome assembly.