GSM9886504: HiC GN, ctrl, FANS, IVE+day2, rep2; Mus musculus; Hi-C
The cerebellum from postnatal day 6 or 22 mice or cerebellar vermis from mice at day 1 to 28 after electroporation were removed and microdissected. For electroporated samples, electroporation efficiency was assessed under a stereomicroscope and mCherry+/NeuN+ nuclei were enriched using fluorescence activated nuclei sorting with a SH800S cell sorter (Sony). The genomic DNA was digested with MboI and re-ligated after the biotin fill-in reaction, followed by sonication. The fragmented DNA was then subjected to biotin-IP. Hi-C libraries were prepared with the NEBNext Ultra™ II DNA Library Prep Kit (#E7645).