whole-genome sequencing of human: cfDNA pool from patients with brain cancer
Cell-free DNA (cfDNA) was extracted from plasma using a phenol-chloroform-based method, followed by ethanol precipitation and resuspension in TE buffer. Some samples were extracted using a commercial kit following the manufacturers protocol. Paired-end sequencing was performed on a NextSeq 550 using High-Output or Mid-Output kits, with cfDNA samples pooled by cancer type. Libraries were prepared with NEB Ultra End Prep, IDT UMI adapter ligation, PCR amplification, and bead-based clean-up