GSM8546092: WT_Female_Day2_2; Mus musculus; RNA-Seq
RNA (minimum of 200ng) was extracted from tissue samples or purified cell populations using QIAzol and miRNeasy RNA extraction kits (QIAGEN) according to manufacturer's instructions. Full length cDNA libraries were prepared using the ONT cDNA-PCR Sequencing V14 - Barcoding kit (SQK-PCB114.24). Here, cDNA RT adapters were ligated to 3' ends (polyA tails) of transcripts, prior to stand switching cDNA synthesis and a 13 cycle PCR reaction with barcoded primers, labelling each cDNA sample with a unique DNA barcode. Barcoded cDNAs were pooled at equimolar ratios before final adapter ligation and sequencing on R10.4.1 flowcells an ONT PromethION sequencer. Two Promethion flow cells in total, with 8 samples per flow cell (2 replicates per condition -Day 0, Day 2, WT, Malat1-/-).