GSM9376838: BGLT3 KD rep1, Day8; Homo sapiens; RNA-Seq
The cells were collected and RNA was harvested using Trizol reagent. RNA libraries were prepared for sequencing using standard Illumina protocols. Total RNA was extracted according to the manufacturer's protocol. Fragmentation of the extracted total RNA was performed using RNA Fragmentation Reagents. To Purify the Fragmented NRO-RNA Through p-30 RNase-free Spin Column. Newly synthesized BrdU-labeled RNA was immunopurified using agarose Beads conjugated BrdU antibody. Fragmented RNA underwent end repair, 3' adapter ligation, reverse transcription, and 5' adapter ligation using the Illumina TruSeq Small RNA kit. The resulting cDNA libraries were amplified by PCR, purified, and size-selected (typically 150-300 bp) using agarose gel electrophoresis. Small RNA libraries were prepared using the TruSeq Small RNA Sample Preparation Kit (Illumina, Cat. No. RS-200-0048) according to the manufacturer's instructions. The cells were collected and RNA was harvested using Trizol reagent. RNA libraries were prepared for sequencing using standard Illumina protocols.