GSM9500002: Liver_Veh_2; Mus musculus; RNA-Seq
Mouse BMDM cell-culture experiments, RNA was isolated using RNA STAT 60 (Amsbio) as per the manufacturer's protocol. ~ 2000 ng of RNA was converted to complementary DNA using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Mouse BMDM were lysed with RNA STAT 60 (Amsbio) and ~50 ng per uL of RNA was submitted to the UCLA's Technology Center for Genomics & Bioinformatics (TCGB) core for library preparation and 2x100 paired-end sequencing.