16S rRNA Sequence: Anaerobic microbial isoprene Degraders
DNA was extracted from 1 ml liquid enrichment culture using a FastDNA TM Spin Kit for Soil (MP, USA). V4 region of the 16S rDNA gene was carried out by PCR using the modified universal primers 515F (5-AATGATACGGCGACCACCGAGATCTACACGCT XXXXXXXXXXXX TATGGTAATT GT GTGYCAGCMGCCGCGGTAA-3) and 806R (5 CAAGCAGAAGACGGCATACGAGAT AGTCAGCCAG CC GGACTACNVGGGTWTCTAAT-3), which specifically targeted bacteria and archaea. The 515F and 806R primers were modified using a barcode introduced into the forward primer 515F. The PCR products were purified using AMPure XP (Beckman Coulter) following the Illumina MiSeq protocol. Subsequently, the samples were sequenced on an Illumina MiSeq Sequencer (Illumina, USA) employing paired end 16S community sequencing on the Illumina platform.