GSM9307504: Induced cortical neurons, 5-Azacytidine, replicate 2; Homo sapiens; RNA-Seq
RNA was extracted and purified from cultured cells using the Direct-zol™ RNA Miniprep Plus (R2071, Zymo Research, Irvine, CA, USA). RNA concentration was determined with the Qubit RNA BR Assay Kit (Q10210, ThermoFisher Scientific, Waltham, MA, USA) and Qubit 4 Fluorometer (Q33226, ThermoFisher Scientific, Waltham, MA, USA). RNA quality was assessed using the RNA 6000 Nano (5067-1511, Agilent, Santa Clara, CA, USA) on the Agilent 2100 Bioanalyzer (G2939BA, Agilent, Santa Clara, CA, USA). RNA-seq libraries were prepared from 1000 ng of total RNA subjected to rRNA depletion with the NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) (E7405L, New England Biosciences, Ipswich, MA, USA). Strand specific library preparation was performed using the NEBNext II Ultra Directional Library Prep Kit (E7760S, New England Biosciences, Ipswich, MA, USA)