GSM8506723: BMPC_IgG_P3_tom-_4; Mus musculus; RNA-Seq
Splenic plasma cells were FACS-purified (CD138+ TACI+) and sorted directly into beta-mercaptoethanol-containing RLT (Qiagen) Lysis buffer. Total RNA was isolated with the RNeasy Micro Kit (Qiagen). Sequencing libraries were constructed with the Takara Clontech Smart-Seq v4 Plus kit according to manufacturers instructions and sequenced on Illumina Hiseq X instruments.