Whole-exome sequencing of parental and radioresistant human cell line models of prostate cancer
Whole-exome sequencing of parental and radioresistant human cell line models of prostate cancer
For each sample, pellets containing up to 10^6 cells were collected by trypsinisation and centrifugation, followed by snap-freezing on dry ice. DNA was extracted from pellets using Qiagen AllPrep DNA/RNA Mini kit (Cat. No. 80204), according to the manufacturer's instructions. Individual libraries were prepared from 100ng of each DNA sample using the TruSeq Exome Library Prep kit, according to the manufacturer's instructions.