short-read dna sequencing of brl2
The brl2 mutant allele was isolated from a N-Nitroso-N-methylurea (NMU) mutagenized M2 population of S. viridis (Huang et al., 2017; Yang et al., 2021a). NMU mutagenesis was used on wild type A10.1 Setaria viridis to generate and screen inflorescence development mutants. The brl2 mutant was backcrossed to the wild type inbred line A10.1 and this F1 was allowed to self. The F2 population segregated 1:3 for the mutant phenotype. To identify the causal mutation, we performed Bulked Segregant Analysis (BSA) coupled to whole-genome shotgun sequencing (Klein et al., 2018a; Yang et al., 2021a). We pooled an equal amount of DNA from 30 mutant individuals and prepped a DNA sequencing library for Illumina HiSeq 2500 SR 100 bp.