GSM9567230: HIF1A knockout, donor LRS-42; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNeasy Mini Kit (Qiagen). DNase treatment and library preparation were performed by the Yale Center for Genome Analysis. Poly(A)-selected libraries were prepared using the Illumina TruSeq Stranded mRNA Library Preparation Kit at the Yale Center for Genome Analysis. Samples were sequenced on an Illumina NovaSeq 6000 platform to generate 150-bp paired-end reads (~25 million reads per sample)