GSM9461295: BD8, biol rep3; Homo sapiens; RNA-Seq
RNA was isolated using RNeasy plus mini kit (QIAGEN), according to the manufacturer's protocol.  RNA concentrations were measured by Tape Station. total RNA was fragmented followed by reverse transcription and second strand cDNA synthesis. The double strand cDNA was subjected to end repair, A base addition, adapter ligation and PCR amplification to create libraries. Libraries were evaluated by Qubit and TapeStation. Sequencing libraries were constructed with barcodes to allow multiplexing of 24 samples