GSM8825567: RA-136 GBM 357 ATAC-seq ML228; Homo sapiens; ATAC-seq
50,000 cells were spun down at 500 x g for 5 minutes at 4°C, resuspended in ATAC-resuspension buffer (0.1% NP40, 0.1% tween-20, 0.01% digitonin), incubated on ice for 3 minutes and washed. Nuclei were pelleted at 500 x g at 4C for 10 minutes. ATAC-seq libraries were created using 50,000 cells, cells were resuspended in ATAC-resuspension buffer, transposed using Illumina Nextera Tn5 Transposase, DNA purified with Qiagen MinElute PCR Purification Kit and amplified for 12 cycles.