Illumina NovaSeq 6000 paired end sequencing
Total DNA was extracted from a mesophilic digestate sample. The libraries were prepared using the Illumina DNA Prep Library Kit (Illumina, San Diego, CA, USA). Fragment size distribution and library concentration were assessed with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) using the High Sensitivity DNA Kit (Agilent Technologies). Library concentrations were further determined using the Qubit dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA).