GSM9136912: P30_rep3; Homo sapiens; OTHER
DNA extraction was performed by adding 5 μL of proteinase K, 100 μL of buffer L/B, and 20 μL of DNA extraction beads, followed by incubation at 55 ℃ for 10 min. For library amplification, 15 μL of extracted DNA was mixed with 25 μL of 2 × CAM, P5 Primer, and P7 Primer. The H3K27ac library was amplified for 13 cycles, while the H3K4me1 and H3K4me3 libraries were amplified for 11 cycles. To purify the PCR products, 2 × VAHTS DNA Clean Beads was added, followed by incubation at room temperature for 5 min. After purification, paired-end sequencing was performed on the NovaSeq 6000 platform with three biological replicates at Novogene Biotech Co., Ltd.