GSM9736680: CD34 Donnor 3 Day 3 gRNA NINJ1/2; Homo sapiens; RNA-Seq
Total RNA was extracted from tail larvae or human CD34+ cells using TRIzol reagent (Invitrogen) following the manufacturer's instructions Total RNA from human samples was processed using the QuantSeq 3' RNA-Seq V2 FWD kit with Unique Dual Indices, 12 nt, following the manufacturer's recommendations. Libraries were generated from polyadenylated RNA using oligo-dT priming and included unique molecular identifiers to enable the identification of PCR duplicates. Library quality control confirmed an appropriate insert size, greater than 200 bp, and absence of adapter dimers. The libraries were sequenced on an Illumina NextSeq 2000 platform, generating single-end reads of 110 bp.