NC3 (CRX2109371)
The extracted mRNA was enriched using mRNA capture beads. After purification with beads, the mRNA was fragmented by heating. The fragmented mRNA was used as a template to synthesize the first strand of cDNA using a reverse transcription enzyme mix. During synthesis of the second strand, end repair and A-tailing were performed. Adapters were then ligated, and Hieff NGS DNA Selection Beads were used for purification to select target fragments.