Bisulfite-Seq of Homo sapiens: plasma cfDNA and tumor biopsy DNA
PCR products were pooled, and the pooled library was then purified twice using AMPure XP Beads (Beckman Coulter Life Sciences, CA, USA) and quantified by RealTime PCR using NEBNext Library Quant Kit for Illumina (New England Biolabs, MA, USA). Barcoded libraries from all samples were sequenced on the Illumina platform using MiSeq Reagent Nano Kit V2 using a 250x2 paired-end sequencing protocol (Illumina, CA, USA).