GSM9917686: zb_day15_F_DMSO_T_rep8; Danio rerio; RNA-Seq
Zebrafish brains previously frozen in 300 µL Trizol were thawed and homogenized with a pellet pestle. Following addition of 350 µL Trizol and 200 µL chloroform, samples were centrifuged 12,000 g at 4°C for 15 minutes. The aqueous phase containing RNA was removed and mixed with one volume of 70% ethanol. Mixture was transferred to a Qiagen RNeasy spin column, and RNA was isolated per Qiagen RNeasy Plus (Qiagen, Inc. 74134) protocol and cleaned with Qiagen RNase-free DNase1 (Qiagen, Inc 79254). RNA was eluted in 30 µL RNase-free water and RNA concentration was quantified by NanoDrop spectrophotometer (Fisher Scientific, MA, USA). Zymo Ribo-Free Total RNA Seq