"Tridacna crocea (control, 2-month) L02 kidney"
Total RNA was extracted from tissue samples using TRIzol reagent (Thermo Fisher Scientific). Extracted RNA was cleaned with an RNeasy Mini Kit (QIAGEN) following the manufacturers instructions with DNase treatment. Sequencing libraries were prepared using the Collibri 3 mRNA Library Prep Kit for Illumina (Thermo Fisher Scientific). Sequencing adaptors were added by PCR amplification according to the manufacturers protocol.