GSM9346892: uninfected; Mus musculus; Influenza A virus (A/Puerto Rico/8/34/Mount Sinai(H1N1)); RNA-Seq
Heart tissue nuclei were isolated using the nucleus isolation kit according to the manufacturer's instructions (10x). After nucleus counting and viability control, the library was constructed on the Chromium 10x instrument using Chromium single cell 3 'reagent v3.0 kits, followed by sequencing on Illumina HiSeq 2500 instruments, which resulted in approximately 205-209 million reads per sample from approximately 14 000 sequenced nuclei.