CD4 Donor 3 Buffer
Cells were treated for 48 hours with 50 g/ml OxLDL or similar volume of buffer solution. For ATAC-sequencing, samples were repeatedly centrifuged before nuclear pellets were suspended in a transposition reaction mix (25 l 2x TD buffer (from Illumina DNA sample preparation kit), 2.5 l hyperactive Tn5 transposase (from Illumina DNA sample preparation kit), and 22.5 l nuclease-free water) and incubated for 30 minutes at 37C. Transposed DNA was purified with the Qiagen MinElute kit and amplified by PCR.