WGS of mus musculus: mammary tumour
Genomic DNA was isolated from approximately 50,000 freshly FACS-sorted tumor cells or control diploid spleens using a Quick-DNA Microprep Kit (D3020, Zymo Research) according to manufacturers protocol except that the final elution was performed with DNase-/RNase-free water. Genomic DNA was quantified using a Qubit dsDNA High Sensitivity Assay kit (Q32851, ThermoFisher Scientific) prior to library preparation. The final barcoded sequencing libraries were prepared from purified genomic DNA using Illumina Nextera XT Library Preparation Kit (FC-131-1024, Illumina) according to manufacturers protocol using at least 1ng of input.