Oxford Nanopore sequencing of 5hmC-modified synthetic sustech seqs DNA
The cytosine in the library is fully substituted by 5hmC during PCR amplification. Library preparation was performed using the Oxford Nanopore Technologies (ONT) Ligation Sequencing Kit (SQK-LSK110) following the manufacturers protocol. The sequencing was carried out on a Nanopore MinION device. Reads are basecalled with reinforced 5hmC DeepSME basecaller at https://github.com/sparkcyf/DeepSME and could be decoded to binary data via https://github.com/sparkcyf/DeepSME_DNA_Storage_Decode_scripts.