GSM9461573: Maternal Serum 3rd Trimester_Control_05; Homo sapiens; miRNA-Seq
Total miRNA was purified using the Qiagen miRNeasy Mini Kit (Qiagen, Valencia, CA, USA) per the manufacturer's protocol. miRNA concentrations were measured using the NanoDrop 2000c spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). Quality control analysis and quantification were performed using Agilent Technologies 2100 Bioanalyzer (Agilent, CA, USA), and RNA integrity number >7.0 confirmed sample integrity. Sequencing was performed by LC Sciences (Houston, Texas, USA). We used 1 µg of total RNA to prepare small RNA libraries with TruSeq Small RNA Sample Prep Kits (Illumina, San Diego, USA). Single-end 50bp sequencing was conducted on an Illumina Hiseq 2500 system.