16srRNA amplicon sequencing of Hangul gut microbiome
The amplicons generated went through a PCR-cleanup step before a second round of PCR (index PCR) was carried out to attach the dual indices and Illumina sequencing barcodes. This step was performed with Nextera XT Index kit and was followed up with another PCR-cleanup step before proceeding to library quantification, and normalization using Qubit 4 (Thermo Fisher Scientific, Waltham, MA, USA) dsDNA HS Assay.