Cleavage site determination of TS of VemCas12e
To determine the cleavage sites of Cas12e on plasmids, a plasmid with a single PAM was applied to the cleavage system describes above. The linearized products were treated using the same methods to those in PAM screening assays, with the exception that the fragment cover the PAM-distant end after the cleavage was also enriched by two primers paired to the adapter and downstream of the PAM, enabling the cleavage sites determination on the non-target strand. The primers used in PAM screening assays are listed in Supplementary Table 3.