GSM9021039: MM1S_H3K4me3_ChIP_withIL6_4h; Homo sapiens; ChIP-Seq
MM.1S cells were crosslinked with 1% formaldehyde (and 2 mM disuccinimidyl glutarate for ChIP with anti-POU2AF1 and anti-ELL2 antibodies). After quenching, the samples were lysed and sonicated using Bioruptor Pico (Diagenode). For ChIP with reference exogenous genome, spike-in chromatin (Active Motif, 53083) was then added. The protein-bound DNA fragments were then immunoprecipitated with specific antibodies. After reverse-crosslinking, DNA was purified using QIAquick PCR Purification Kit (Qiagen). Libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions.