GSM8825203: MDX594 Vehicle.; Mus musculus; OTHER
Single-cells were loaded to the 10×Genomics Chromium Controller to be partitioned into Gel Beads-in-emulsion (GEMs). The cDNA sequencing libraries were prepared using the Chromium Single Cell 5´ Library and Gel Bead Kit (10X Genomics, Cat. N. 1000006) following manufacturer's instructions. Briefly, after GEM generation and reverse transcription (RT), GEM-RT were cleaned up and cDNA was amplified during 13 cycles to be subjected to quality control and quantification on an Agilent Bioanalyzer High Sensitivity chip (Agilent Technologies). To construct the Gene expression (GEX) library, 25-50 ng of the amplified cDNA were fragmented, end repaired, A-tailed, and sample indexed using the Chromium Single Cell 5' Library Construction Kit (10x genomics, 1000020). For the V(D)J library, T cell receptor sequences were enriched from the amplified cDNA with the Chromium Single Cell V(D)J Enrichment Kits (PN-1000005)