GSM9020118: VAS7564A84; Mus musculus; RNA-Seq
Small intestines were cut into small pieces digested and isolated following the instructions provided in Miltenyi Biotec Lamina propria dissociation kit protocol, 130-097-410. lamina propria and intraepithelial cells were resuspended in FACS buffer after centrifugation. Single cell suspensions were stained with lineage exclusion antibodies against CD19, CD3, NK1.1, Ly6G and gated in CD45+, CD11b+, CD64+ then, FACS sorted on an Aria Fusion (BD) with a 100um nozzle. RNA-seq libraries were made using total RNA made with KAPA RNA HyperPrep with RiboErase following manufacturer's instructions (Roche, 08098131702). SMART‑Seq libraries were pooled and sequenced on an Illumina NovaSeq 6000 (2 × 100 bp) (Illumina).