D_F25
Genomic DNA was extracted from mouse fecal samples using the FastPure Stool DNA Isolation Kit. The V3-V4 region of the 16S rRNA gene was amplified with barcoded primers 338F and 806R. The PCR products were purified, quantified using Qubit 4.0, and used to construct sequencing libraries with the NEXTFLEX Rapid DNA-Seq Kit. Final libraries were sequenced on an Illumina Nextseq2027 platform.