GSM8508533: HDBI58 Cord Blood Day 17 GFP high; Homo sapiens; RNA-Seq
Chromium Single Cell 3' Library & Gel Bead Kit v3 (10X Genomics) following manufacturer's protocols Following post GEM-RT cleanup and cDNA amplification, 10ul of clean cDNA was taken for targeted lineage barcode enrichment. To target the barcode, we used a similar strategy to that described in Weinreb et al 2020, Science. Primers targeting the LARRY vector and Illumina overhangs were used, with specific 10x sample indexes to allow for muliplexing of the samples for sequencing. the following primers were used, i5_AATGATACGGCGACCACCGAGATCTACACNNNNNNNNNNACACTCTTTCCCTACACGACGCTCTTCCGATCT and i7_CAAGCAGAAGACGGCATACGAGATNNNNNNNNNNGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTACCGTTGCTAGGAGAGACCATATG. AMPure or SPRI bead cleanup was performed (0.7 ratio). Library quality and size was verified using Aglient Tapestation and libraries were spiked into the original GEX 10x library for sequencing.