GSM8994683: Control 2, DART-WT-treated; Homo sapiens; RNA-Seq
RNA was harvested using the Rneasy Plus Kit (Qiagen). 50ng of total RNA was used for the DART reaction. RNA was repurified using the RNeasy Micro Kit (Qiagen). 3ng of total RNA was used as input for cDNA library construction. cDNA libraries were generated using the SMARTer® Stranded Total RNA-Seq Kit v2 (Takara Bio) following the manufacturer's protocol.