GSM9820617: HepG2 CRISPR screen DMSO, rep1; Homo sapiens; OTHER
Genomic DNA (Monarch Genomic DNA Purification Kit, NEB T3010). sgRNA amplification with Illumina adapters. ~6,000 sgRNA library in pLentiCRISPRv2. Lentiviral transduction MOI 0.25-0.3, puromycin 1 µg/ml 7 days. Single-end 75 bp, Illumina NextSeq 500.