GSM9753313: Col-0, biological replicate 3; Arabidopsis thaliana; RNA-Seq
RNA was isolated and purified from three-week old seedlings using the RNeasy Plant Mini Kit (Qiagen) and treated with Dnase-I. 2 ug of total RNA was used for the construction of sequencing libraries. mRNA was enriched by using oligo(dT) magnetic beads and fragmented into short fragments (about 200 bp). Double strand cDNA was synthesized by using random hexamer-primers and purified with magnetic beads. End reparation and 3'-end single adenine addition was performed. Sequencing adaptors were ligated to the fragments and the fragments were enriched by PCR amplification.