Nanoplatic induced soybean symbiotic functioning
Total RNA was extracted using TRIzol reagent. rRNA was depleted using Ribo-Zero Magnetic kit (EpiCentre). mRNA was fragmented and reverse transcribed into cDNA using random primers. End repair, A-tailing, and adapter ligation were performed followed by PCR amplification for 15 cycles. Libraries were sequenced on Illumina NovaSeq platform using paired-end 150 bp reads.