GSM9677996: mESCs, Mettl5KO_c1; Mus musculus; RNA-Seq
Total RNA was isolated using TRIzol-Chloroform extraction (Thermo Fisher Scientific) followed by iso-propanol precipitation, according to the manufacturer's protocol. Total RNA was ligated to poly-T WarpDemuX RTA adapters for multiplexing (Van Der Toorn et al., 2025) using T4 DNA ligase (NEB) and reverse-transcribed to form RNA-cDNA hybrid using the SuperScript III Reverse Transcriptase (Thermo Fisher Scientific). The end of library preparation was performed using the SQK-RNA004 kit (ONT) according to the manufacturer's instructions. Libraries were loaded onto FLO-MIN004RA flow cells.