GSM9136889: P22_rep1_ATAC; Homo sapiens; ATAC-seq
The cells were centrifuged at 500 g, 4 ℃ for 5 min, and the cell pellet was then resuspended in 50 μl of ice-cold lysis buffer containing 10 mM Tris-HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl2, and 0.1% (v/v) Igepal CA-630. The cell suspension was placed on ice for 2 min to allow cell lysis, followed by centrifugation at 500 × g for 5 min at 4°C to collect the nuclei. The lysed cells were then transferred to sterile PCR tubes and subjected to transposition reaction and library construction using the TruePrep DNA Library Prep Kit V2. For the transposition reaction and library construction, the lysed cell samples were mixed with 5 × TTBL, TTE-Mix V50, and ddH2O according to the kit instructions. The mixture was heated at 37°C for 30 min to facilitate the fragmentation reaction. Afterward, 5 × TAB, PPM, P5 Primer, and P7 Primer were added to the enzymatically treated products purified using VAHTS DNA Clean Beads. The DNA library was obtained by performing 15 cycles of PCR amplification. Subsequently, the library was purified and subjected to paired-end sequencing on the NovaSeq 6000 platform with two biological replicates at Novogene Biotech Co., Ltd.