GSM9675359: Mulitome, WT, E14.5, rep2, GEX; Mus musculus; RNA-Seq
Placental samples were collected from SCNT and naturally fertilized pregnant mice. Pregnant ICR mice at E12.5, E14.5 and E18.5 were humanely sacrificed and their placenta were dissected. Placentas were washed with PBS to remove blood and blotted dry with absorbent paper. The treated placentas were snap-frozen in liquid nitrogen and stored to a liquid nitrogen tank. Placental tissue was thoroughly washed in ice-cold PBS to eliminate blood contaminants and precisely weighed (2-3 g). The tissue was then carefully minced into ~1 mm³ fragments using sterile scissors before enzymatic digestion in pre-warmed DMEM/F12 medium (Gibco) containing 0.5 mg/mL collagenase IV (Thermo Fisher Scientific, 17104019) and 0.2 mg/mL DNase I (Roche 10104159001). The digestion was performed at 37°C for 20 minutes in a shaking water bath with gentle manual inversion every 10 minutes. Libraries were constructed by following the 10X Genomics Chromiun Next GEM Sing Cell Multiome ATAC + Gene Expression protocol (CG000338).