GSM9307105: rat C6 H3K27me3 - 50K cells - Replicate 2; Rattus norvegicus; OTHER
Genomic DNA of mESCs and rat C6 was extracted to perform CUT&Tag using CUT&Tag-direct with CUTAC V.3 (Steven Henikoff, Jorja Henikoff, Kami Ahmad 2021. CUT&Tag-direct with CUTAC. protocols.io https://dx.doi.org/10.17504/protocols.io.bqwvmxe6). CUT&Tag libraries for CTCF, H3K27me3, IgG were then prepared based on the before-mentioned protocol and sent all mESCs and RatC6 samples to the Novogene (illumina Novaseq 6000) for sequencing.