GSM9305514: CD-1 mouse brain, amygdala, sub stimulus; Mus musculus; RNA-Seq
Mouse amygdala tissue was microdissected and pooled by condition. Tissue was homogenized in Nuclei EZ Lysis Buffer with RNase inhibitor using a chilled Dounce homogenizer. The lysate was filtered through a 35 µm strainer and centrifuged. Nuclei were purified using a 25%/29% iodixanol gradient, washed in 2% BSA/PBS with RNase inhibitor, filtered again, stained with Trypan Blue, and counted using an automated cell counter. Single-nucleus suspensions were loaded onto the Chromium Controller and libraries were prepared using the Chromium Next GEM Single Cell 3′ Kit v3.1 (10x Genomics) following the manufacturer's protocol, targeting ~10,000 nuclei per library. Libraries were sequenced on an Illumina NovaSeq 6000 using an S1 flow cell with standard read configuration.