GSM9568113: CB, RNA-seq, R750W, rep1; Homo sapiens; RNA-Seq
Total RNA was then extracted and purified from the GFP+ cells using the FastGene™ RNA Purification Kit (FastGene, FG-8005).After RNA fragmentation, cDNA was synthesized by random hexamer-primed reverse transcription, followed by a second-strand cDNA synthesis with dUTP. Following end repair, add A and adapter ligation, the DNA fragments were amplified by PCR. Libraries were sequenced using Illumina Novaseq 6000 with paired-end mode.