PacBio reads Chelymorpha alternans genome
High molecular weight (HMW) DNA was extracted from two whole adult females using the Monarch HMW DNA Extraction Kit for Tissue. HMW DNA was sheared to approximately 25 kb using Megaruptor 2. A high-fidelity (HiFi) sequencing library was prepared using the SMRTbell Express Template Prep Kit 2.0. Sequencing was conducted on two 8M SMRT Cells on the PacBio Sequel II System.