GSM9258104: mRNAseq_Anthers_b4_WTCol-0_rep1; Arabidopsis thaliana; RNA-Seq
Total RNA was extracted from flower buds (stage 12 and younger), unfertilized ovules (stage 12), and anthers (stage 9) using the Zymo Quick-RNA Miniprep Kit (R1055). 1 μg of DNase I treated total RNA was used for mRNA isolation with the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, Cat# E7490L). Library preparation was performed using the NEBNext Ultra II RNA Library Prep Kit (NEB, Cat# E7770L). Pooled libraries were sequenced using a 50 bp single-end kit (SE50) or paired-end kit (PE50) on the Illumina Nextseq 2000 or Novaseq X plus platforms.