GSM9465068: LS-WFPM0.1 oocyte 2; Mus musculus; RNA-Seq
SMART-seq v4 Ultra-low input RNA kit The single-cell SMART-seq2 RNA-seq libraries were constructed using a modified single-cell tagged reverse transcription sequencing protocol. Briefly, mRNA from each single cell was extracted into the lysis buffer provided by the SMART-seq v4 Ultra-low input RNA kit (634891, TaKaRa, San Jose, CA, USA). mRNAs were then reversed transcribed into complementary DNA（cDNA), which was then pre-amplified and purified. High-quality cDNA products were subjected to tagmentation and library preparation, with multiplexing by Nextera XT Indexes (Illumina). Pooled indexed libraries were sequenced on the NovaSeq X Plus platform with 150 bp pair-end reads by Novogene.