GSM9737912: RhoA HET+RhoA E40Q_REP2(HET_rep2); Mus musculus; RNA-Seq
RNA were harvested using RNeasy Kit (Qiagen). RNA samples were fragmented, and first-strand cDNA was synthesized using random hexamer primers, followed by the second-strand cDNA synthesis. Libraries were prepared through end repair, A-tailing, adapter ligation, size selection, amplification, and purification.