Amplicon sequencing of fungi: rhizosphere soil of Trifolium repens
Environmental DNA was extracted from mineral pouches, rhizosphere soil, roots, and fungal inoculum associated with Trifolium repens grown under greenhouse conditions. The fungal internal transcribed spacer (ITS) region was PCR-amplified using the general fungal primers gITS7 (5-GTGARTCATCGARTCTTTG-3) and ITS4 (5-TCCTCCGCTTATTGATATGC-3). Amplicons were sequenced using paired-end Illumina technology to characterize total fungal community composition in each sample.