GSM9347268: Intestinal Tissue, NEC DMSO, Batch 1, Rep 4; Homo sapiens; RNA-Seq
Cells were lysed with the TRIzol Reagent (Thermo Fisher) in low-binding tubes for gene expression profiling and stored at -80°C. Total RNA was isolated by a Direct-zol Microprep RNA puriﬁcation kit (Zymo Research) with in-column DNase I treatment following the manufacturer's instructions RNA concentrations were quantified using a NanoDrop One spectrophotometer (Thermo Fisher). Equal amounts of total RNA were used as a template to synthesize a first-strand cDNA using the high-capacity cDNA QuantiTect Reverse Transcription Kit (Qiagen) in a 20-μL reaction, and the volume was adjusted to 100 μL with nuclease-free water (Thermo Fisher). Quantitative real-time PCR (qRT-PCR) results were obtained using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) and specific primers on a CFX Opus Real-Time PCR Detection System (Bio-Rad).