GSM9347493: Control -3; Mus musculus; RNA-Seq
Live single cell suspensions from mouse hippocampus and fimbria were prepared using the same methods detailed in Lee et al.(doi 10.1016/j.celrep.2021.110158). Mice were perfused with ice-cold PBS and 5 µg/ml Actinomycin D was added during digestion with Neural Tissue Dissociation Kit (Miltenyi) at 37℃. Live single cells were collected by FACS with BD FacsAria Fusion sorters. Cell viability was >70%. Single cell suspensions were adjusted to 2,000 cells/µl. Libraries were prepared with Chromium Next GEM Automated Single Cell 3' Reagent Kit v.3.1 and Chromium Connect (10X Genomics) following manufacturer's instructions. Libraries were quantified by Qubit dsDNA HS assay kit (ThermoFisher) and profiled by Bioanalyzer High Sensitivity DNA kit (Agilent Technologies), and sequencing was performed with NovaSeq6000 (Illumina).