GSM9497355: NN_6; Bos taurus; RNA-Seq
Total RNA isolation was carried out using Trizol in conjunction with an on-column DNase digestion step, as per the manufacturer's guidelines. Strand-specific libraries were then constructed using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (New England BioLabs®, Ipswich, MA, USA).