scRNA-seq of WT thymus sample 1(cDNA)
Single-cell RNA sequencing was performed on thymus from wild-type (WT) and CD4-Cre;Eif4g2 flox/flox (cKO) mice (n=2 per genotype). Briefly, tissues were dissociated into single-cell suspensions using collagenase IV. Single cells were captured, lysed, and barcoded. Sequencing libraries were generated according to the manufacturer's instructions and sequenced on a DNBSEQ-T7 platform in paired-end mode.