BL_IVF_Methyl_2 (CRX1622228)
After eliminating the zona pellucida, bisulfite conversion was performed according to the protocol provided in the EZ-96 DNA Methylation-Direct MagPrep kit (Invitrogen, D5045). The converted DNA was purified using Zymo-Spin columns with 10 ng of tRNA (Roche) added during the purification process. Whole-genome libraries were then constructed. Libraries that passed quality control were sequenced on the Illumina HiSeqX Ten platform.