GSM8454435: WT3 WGBS; Mus musculus; Bisulfite-Seq
Genomic DNA was extracted using QIAamp DNA micro kit (Qiagen) and bisulfite treated using EZ DNA Methylation Gold Kit (Zymo Research). Genomic DNA spiked with lambda DNA were fragmented to 200-400 bp. The DNA fragments were bisulfite treated to generate single strand DNA. Methylation sequencing adapters were ligated, followed by double strand DNA synthesis. The library was ready after size selection and PCR amplification.