GSM9886356: cko2; Mus musculus; RNA-Seq
Total RNA from the sciatic nerve was extracted using the Universal RNA Extraction CZ Kit (RNC643, ONREW). RNA quantity was analyzed using Qubit 4.0 (Invitrogen) and quality examined by electrophoresis on a denaturing agarosegel. Only samples with an RNA concentration ≥10 ng/μL, and a total amount ≥ 500 ng were selected for library preparation. Libraries were prepared using the VVAHTS® Universal V8 RNA-seq Library Prep Kit for Illumina (NR605-0, Vazyme)