PS_3 (CRX2123434)
mRNA was enriched from total RNA using poly-T oligo-attached magnetic beads (Beckman Coulter, USA). First-strand cDNA and second-strand cDNA were synthesized, followed by PCR amplification using universal primers, an index (X) primer, and Phusion high-fidelity DNA polymerase. The PCR products were purified with the AMPure XP system