GSM8603293: CLP-d; Mus musculus; RNA-Seq
About 30mg of liquid nitrogen samples were ground and total RNA was extracted using TRIzol™ Reagent. According to the Total amount of Total RNA, 0.1-1ug of total RNA was taken. mRNA was isolated using the NEBNext® Poly(A) mRNA Magnetic Isolation Module kit. The mRNA Library was then constructed using the NEBNext® Ultra™ II mRNA Library Prep Kit for Illumina® Library Construction Kit.Library sequencing was performed using a NovaSeq 6000 instrument with a matching NovaSeq S4 reagent kit