RNA-seq of Montipora capitata: 28_9phf
Using 150-190 ng of total RNA, rRNA-depleted RNA-Seq libraries were prepared using the Zymo-Seq RiboFree Total RNA Library Kit (R3000) with 15 cycles of library amplification (98C for 20 seconds, 60C for 30 seconds, 72C for 30 seconds). Library concentration and size was determined with an Agilent Tapestation 4200. The libraries were sequenced at the Oklahoma Medical Research Foundation NGS Core on an Illumina NovaSeq X Plus in a 2x150 bp configuration.