C4
Total genomic DNA was extracted from human fecal samples using the QIAamp PowerFecal Pro DNA Kit following the manufacturer's protocol. The quantity and quality of the extracted DNA were assessed using a Qubit Fluorometer and Agilent 4200 TapeStation system. Sequencing libraries were constructed with the Illumina DNA Prep Kit according to the manufacturer's instructions. Briefly, DNA was fragmented by ultrasonication, followed by end repair, A-tailing, and adapter ligation. The libraries were then amplified by PCR for 8 cycles. The final libraries were quantified and qualified by Qubit and Agilent 4200 TapeStation. Sequencing was performed on an Illumina platform to generate 50-bp paired-end reads.