GSM9568382: S143P-corrected ATAC-seq 1; Homo sapiens; ATAC-seq
Briefly, the Cell membrane was permeabilized with nuclear lysis buffer (10 mM tris, 10 mM NaCl, 3 mM MgCl2, and 0.5% IGEPAL-630). Then, cells were resuspended in 50uL of transposase reaction mixture (22.5uL nuclease-free water, 25uL of TD buffer, and 2.5uL of TDE1 enzyme, Illumina #20034197). Transposition was carried out at 37°C for 22 min, followed by DNA purification with DNA Clean and Concentrator-5 (Zymo Research) according to the manufacturer's recommendation. Following purification, library fragments were PCR amplified with Nextera XT v2 adapter primers. Multiplexed and pooled library was sequenced on the Novaseq X Plus with paired ends of 50 nucleotides per the manufacturer's instructions. The