GSM8994527: PBMC from C08 sample; Homo sapiens; RNA-Seq
Peripheral blood was collected from all participants, and SepMate (STEMCELL Technologies, ST-8645, Vancouver, Canada) was used for PBMC isolation. T and B cells were sorted from PBMC using a BD Melody cytometer. After 30-min incubation at 4 °C with sample tags and Abseq antibodies, the cells were washed and enumerated using a hemocytometer with Trypan blue. Cell viability was assessed, and the total cell number was quantified using a BD Rhapsody Scanner. A total of 20,000 cells were targeted and loaded onto BD Rhapsody cartridges, allowing single cells to remain stationary. If sample tags were used for pooled cells from two samples, we adjusted the target cell number accordingly. The libraries were prepared according to the BD Rhapsody protocol using the WTA amplification kit. They were then quantified and quality-assessed on an Agilent TapeStation.