ac4C-seq MC38 Ctrl-con (CRX1466610)
Polyadenylated RNA enrichment was conducted using VAHTS mRNA Capture Beads from the total RNA.A portion of 35% of the polyadenylated RNA (PolyA+ RNA) was reserved as a control sample. The remaining RNA was subjected to reduction treatment with 100 mM sodium borohydride(NaBH4).Following the treatment, the NaBH4-reduced RNA was precipitated and resuspended in RNase-free water, denoted as "BH".Both the RNA were used as input for RNA sequencing library preparation utilizing some components of the KCTM Digital mRNA Library Prep Kit.The library PCR products corresponding to fragments ranging from 200 to 500 base pairs were enriched and sequenced on a DNBSEQ-T7 with PE150 mode.