snRNA-seq of Homo sapiens: pancreatic tumor
Chromium Next GEM Single Cell 3 Kit v3.1 (10x Genomics) was used to prepare snRNA-seq libraries according to the manufacturers instructions. Per sample, nuclei were loaded on individual lanes on a 10x Chromium chip. cDNA and sequencing libraries were assessed for sample size and quality using the HS DNA Kit (Agilent) on an Agilent Bioanalyzer 2100.  Libraries were sequenced on an Illumina NovaSeq 6000 (Novogene GmbH, PE150-S4; paired end, dual index) targeting 50,000 read pairs per nucleus.