16S rRNA sequencing of the cecal content from wild-type and Aida knock-out mice
Genomic DNA was extracted using the MagBeads Fecal DNA Kit (116570400, MP Biomedicals, Santa Ana, CA, USA). The V3-V4 hypervariable regions of bacterial 16S rRNA genes were amplified was analyzed using an Illumina HiSeq2500 platform and sequenced on an Illumina HiSeq2500 platform.