Tridacna crocea MYK01 adductor muscle
Total RNA was extracted from tissue samples using TRIzol reagent (Thermo Fisher Scientific). Extracted RNA was cleaned with an RNeasy Mini Kit (QIAGEN) following the manufacturers instructions with DNase treatment. Intact poly(A)+ RNA was isolated with NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). RNA-seq library was then prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (New England Biolabs).