GSM9347309: Whole brain from 6 stage47 tadpoles, ap3b2 mosaic F0 CRISPant, replicate 2; Xenopus laevis; RNA-Seq
Whole brains were dissected from groups of 6 tadpoles at stage 47 onto 2 mL eppendorf tube  caps, removing as much liquid as possible, and spun breifly to collect the tissue into 2 mL tubes placed on dry ice. Samples were mechanically homogenised and total RNA was purified using Qiagen mini RNeasy kit with on column DNase. 1 ug of total RNA was used to make TruSeq stranded (reverse) mRNA libraries (Otago Genomics) which were sequenced using an Illumina Nextseq 2000 P3-200 XLEAP kit generating 2 x 100bp paired end reads.