BRB-seq of tracked male N. furzeri (GRZ): young/middle-aged/old; 8 organs; fast/slow aging trajectory
This library was prepared using the BRB-seq platform with the Mercurius Protocol (Alithea Genomics). Individual sample-specific barcodes and unique molecular identifiers (UMIs) embedded in Read 1 (R1) were added during reverse transcription. Samples were then pooled for all subsequent steps of library prep. Read 1 contains a 14 bp sample barcode at positions 114, followed by a 14 bp UMI at positions 1528, a polyT region, and the 3 cDNA insert. Read 2 contains the cDNA sequence. The barcoded cDNA from all samples was pooled and sequenced as a single library. Adaptors were trimmed from raw sequencing FastQ files using Cutadapt (version 3.1) for removing the last 122 bases of each Read 1 sequence (with parameters -u -122). List of samples with barcodes: 0_137_cohort3_testis_old_     TTAAGCTGCCTTAA 0_137_cohort3_skin_old_ TTCCAACCGGACAG 0_137_cohort3_spleen_old_     ATACCTTAATCTCC 0_137_cohort3_liver_old_      TCCAATAGGTCCAG 0_137_cohort3_kidney_old_     ACACAGCACGATAG 0_137_cohort3_heart_old_      CTCAGTCACACGAG 0_137_cohort3_gut_old_  CCGGTTCCTAGTGA 0_137_cohort3_brain_old_      TCCAGAACTGAGGC 8_146_cohort7_testis_old_     ATATCACAGTCGCA 8_146_cohort7_skin_old_ TATACGCGACATCG 8_146_cohort7_spleen_old_     TACCGGTATGGTCC 8_146_cohort7_liver_old_      TATTGAGTCCGAGA 8_146_cohort7_kidney_old_     CTCAACCGGACGGA 8_146_cohort7_heart_old_      TCGCGGATTAGGCA 8_146_cohort7_gut_old_  ACGATATGCTCTGC 8_146_cohort7_brain_old_      TAACAGTCCGTTCA 3_146_cohort7_testis_old_     CTGTCGAGTAGCAG 3_146_cohort7_skin_old_ CATAATTCTATGCC 3_146_cohort7_liver_old_      CACACGCAGTATCA 3_146_cohort7_kidney_old_     ATAGGCCTTCGAGG 3_146_cohort7_heart_old_      TCATCTGCGCACAC 3_146_cohort7_gut_old_  AACTTCCGGCGTGC 3_146_cohort7_brain_old_      TCATCTAGGTGGAA 8_137_cohort7_testis_old_     TAGCACGCGATGAG 8_137_cohort7_skin_old_ AACACAACCTTCCG 8_137_cohort7_spleen_old_     CACCTGTTGGTTAA 8_137_cohort7_liver_old_      CTACTGCATAGTAG 8_137_cohort7_kidney_old_     CACGACATGCGAGC 8_137_cohort7_heart_old_      AATGCCGTATTGAC 8_137_cohort7_gut_old_  TAGCTTCTCCAGGC 8_137_cohort7_brain_old_      CAAGACCTTGGCCA 7_137_cohort6_testis_mid_slow AATACGGAGACGGC 7_137_cohort6_skin_mid_slow   CCGAGGCTTCCTAG 7_137_cohort6_spleen_mid_slow CTTGATACCGGCGC 7_137_cohort6_liver_mid_slow  ACACTATACACGAG 7_137_cohort6_kidney_mid_slow TATGAGCACACGAC 7_137_cohort6_heart_mid_slow  TAACCTGTTCAAGA 7_137_cohort6_gut_mid_slow    TACGATCGTGATCA 7_137_cohort6_brain_mid_slow  TCCGCCGCACTCAA 5_137_cohort6_testis_mid_slow TTCTCTCCGGTTCA 5_137_cohort6_skin_mid_slow   ATCATCGCGCGACG 5_137_cohort6_spleen_mid_slow ACTAGAGCGTGTGA 5_137_cohort6_liver_mid_slow  AATCCAACTCGAAG 5_137_cohort6_kidney_mid_slow CCAACTCGCCAGGA 5_137_cohort6_heart_mid_slow  TCTCACATCACGAG 5_137_cohort6_gut_mid_slow    CAAGCCAGTTCCGG 5_137_cohort6_brain_mid_slow  TAGTGATCTGTAGC 2_137_cohort6_testis_mid_slow ATTCGGTCCGATGC 2_137_cohort6_skin_mid_slow   AAGGCAAGTTGTGA 2_137_cohort6_spleen_mid_slow ATGGATCGTTAGGA 2_137_cohort6_liver_mid_slow  CAGATCTCAGACAC 2_137_cohort6_kidney_mid_slow CATGGAATGTGTAA 2_137_cohort6_heart_mid_slow  ATATTGTAAGGTGG 2_137_cohort6_gut_mid_slow    ATCACGAGCGACGC 2_137_cohort6_brain_mid_slow  TATTACCGCTCAGG 11_146_cohort6_testis_mid_slow      ACTCATGTCAGACA 11_146_cohort6_skin_mid_slow  ATGCACGATCGAGC 11_146_cohort6_spleen_mid_slow      AACACCTTCTCCGC 11_146_cohort6_liver_mid_slow AAGCCAACAGTTGG 11_146_cohort6_kidney_mid_slow      TCTCCATTATACAG 11_146_cohort6_heart_mid_slow TCGTGTCGCATGCA 11_146_cohort6_gut_mid_slow   ATGGCAACGGCTCA 11_146_cohort6_brain_mid_slow TCCTAATTCTAAGG 10_146_cohort6_testis_mid_slow      ACTACGTCGACACG 10_146_cohort6_skin_mid_slow  CATACGGCCTCTAA 10_146_cohort6_spleen_mid_slow      TTGACCAAGATTAC 10_146_cohort6_liver_mid_slow TAACCGTCTCTGGC 10_146_cohort6_kidney_mid_slow      TCCACAGAACCGGC 10_146_cohort6_heart_mid_slow ACTACTCGATCTAG 10_146_cohort6_gut_mid_slow   TCATTCTACTTGGC 10_146_cohort6_brain_mid_slow ACGTACGCGCATGC 6_146_cohort6_testis_mid_slow TTGGTGAGAACCGC 6_146_cohort6_skin_mid_slow   CATACTATGTAGAC 6_146_cohort6_spleen_mid_slow TCAGAACAGGTTAA 6_146_cohort6_liver_mid_slow  AACCTTCTAGAGAA 6_146_cohort6_kidney_mid_slow TCCGCGATCTCAAC 6_146_cohort6_heart_mid_slow  AACAACCAAGCGGC 6_146_cohort6_gut_mid_slow    ACACGATCTGACGA 6_146_cohort6_brain_mid_slow  CATGCTCACGCAGC 4_146_cohort6_testis_mid_slow ACATGCTATACTAC 4_146_cohort6_skin_mid_slow   TATTCCTAGTTCGA 4_146_cohort6_spleen_mid_slow ACAACTTAGCCTAA 4_146_cohort6_liver_mid_slow  ACGCCTGTTGGAGG 4_146_cohort6_kidney_mid_slow CTCCGCAGTTGGAG 4_146_cohort6_heart_mid_slow  TTGACCTTGCTGGA 4_146_cohort6_gut_mid_slow    CCTACAGGTCGAGG 4_146_cohort6_brain_mid_slow  CTCCTGCGGTCCAA 2_146_cohort6_testis_mid_slow CACTACGCGCTCAC 2_146_cohort6_skin_mid_slow   CATCTATCTGCGCG 2_146_cohort6_spleen_mid_slow CCACCGTTAAGACA 2_146_cohort6_liver_mid_slow  ACATTATTGGCCAA 2_146_cohort6_kidney_mid_slow ATATACACATGAGC 2_146_cohort6_heart_mid_slow  CCGGATGGTGGTAC 2_146_cohort6_gut_mid_slow    AATCAAGTGTGAAC 2_146_cohort6_brain_mid_slow  CCGCGTATTGCAAC 9_147_cohort6_testis_mid_fast AAGTGGTATTGACA 9_147_cohort6_skin_mid_fast   CCACTTCTTGTTGG 9_147_cohort6_spleen_mid_fast CCATGACCACCGGA 9_147_cohort6_liver_mid_fast  TCGCAATTACCTAA 9_147_cohort6_kidney_mid_fast CTACGAGTCATAGA 9_147_cohort6_heart_mid_fast  ACAGCGTACGCGCA 9_147_cohort6_gut_mid_fast    CTGTGATTCGGACC 9_147_cohort6_brain_mid_fast  CTTACTGACAAGCC 8_147_cohort6_testis_mid_slow AAGGAGAAGAATGC 8_147_cohort6_skin_mid_slow   TTGTCGGTTGAGGC 8_147_cohort6_spleen_mid_slow TCCAACAGTGTTGG 8_147_cohort6_liver_mid_slow  TTCCGATGTCCGGC 8_147_cohort6_kidney_mid_slow TAGTGTGGTAGACC 8_147_cohort6_heart_mid_slow  TACTATGCACGACG 8_147_cohort6_gut_mid_slow    CCAGAGGTAATGGA 8_147_cohort6_brain_mid_slow  CTTAATCCATATCC 6_147_cohort6_testis_mid_fast ATAAGGACCGGAGG 6_147_cohort6_skin_mid_fast   TCTTGTACCGGACC 6_147_cohort6_spleen_mid_fast ATCCTTATACGTGC 6_147_cohort6_liver_mid_fast  TTGATCCGTGAAGC 6_147_cohort6_kidney_mid_fast AAGAGTCGGATAGG 6_147_cohort6_heart_mid_fast  TTATAACTGGCAAC 6_147_cohort6_gut_mid_fast    TCAATTAAGGCACG 6_147_cohort6_brain_mid_fast  TTAGACGCCGGTAA 1_147_cohort6_testis_mid_fast TCATGCGCATGTAG 1_147_cohort6_skin_mid_fast   CACACACGAGATGC 1_147_cohort6_spleen_mid_fast CACACACATAGCGA 1_147_cohort6_liver_mid_fast  AATAGTGTTCAACC 1_147_cohort6_kidney_mid_fast CCTTGCCAGAGTGG 1_147_cohort6_heart_mid_fast  AAGAAGAGTGGACC 1_147_cohort6_gut_mid_fast    CCACACCTTCAGGC 1_147_cohort6_brain_mid_fast  ACCGCCTTGGCTAG 0_147_cohort6_testis_mid_slow TTAAGCCGAACAAG 0_147_cohort6_skin_mid_slow   CATGCGTAGCGCAC 0_147_cohort6_spleen_mid_slow CCAGAGGTTGCCAG 0_147_cohort6_liver_mid_slow  TTAGCCTAACTCGC 0_147_cohort6_kidney_mid_slow CATTCCTCTAGCCG 0_147_cohort6_heart_mid_slow  CCGTCCTTCTTCAC 0_147_cohort6_gut_mid_slow    ATCGTGTGTGGCCA 0_147_cohort6_brain_mid_slow  ATGTCTAGTATAGC 5_147_cohort6_testis_mid_slow TTGATTACCGTAAC 5_147_cohort6_skin_mid_slow   ATAACCAACTAGGA 5_147_cohort6_spleen_mid_slow ACGACAGTAGTCGC 5_147_cohort6_liver_mid_slow  ATTGAACTCCATCC 5_147_cohort6_kidney_mid_slow ACTTGAGCAACCGG 5_147_cohort6_heart_mid_slow  CCTCGGTTGAAGCC 5_147_cohort6_gut_mid_slow    TATCGATCATCTAC 5_147_cohort6_brain_mid_slow  CACTAGATATCACA 3_147_cohort6_testis_mid_fast CCTAACTCGCCAGA 3_147_cohort6_skin_mid_fast   CCGAACAACCGTGA 3_147_cohort6_spleen_mid_fast CAGAGACACTCGCA 3_147_cohort6_liver_mid_fast  AATCCGTGTCTTAA 3_147_cohort6_kidney_mid_fast AAGGATGCCAGTGG 3_147_cohort6_heart_mid_fast  TCAGAGTAGTGCAG 3_147_cohort6_gut_mid_fast    CATTCACGCTTGAA 3_147_cohort6_brain_mid_fast  CTTCGGTACACTAA 2_147_cohort6_testis_mid_slow TAATCGCCTACAGA 2_147_cohort6_skin_mid_slow   CAGCCTGGCTTAGG 2_147_cohort6_spleen_mid_slow AACTCTTCGGTAGG 2_147_cohort6_liver_mid_slow  ACTAACCTGGCCGG 2_147_cohort6_kidney_mid_slow CCTTAACACGGAGA 2_147_cohort6_heart_mid_slow  CCAACCGAACGGCA 2_147_cohort6_gut_mid_slow    ATCGAGCAGTGTAC 2_147_cohort6_brain_mid_slow  CTGTTCGGAGGTCA 7_146_cohort6_testis_mid_fast CTTGGAGTGGCACA 7_146_cohort6_skin_mid_fast   CCTGTGATTACACC 7_146_cohort6_spleen_mid_fast CATCGTAGCAGAGC 7_146_cohort6_liver_mid_fast  TATATGCTCGCTCA 7_146_cohort6_kidney_mid_fast TAGACGATAGTACA 7_146_cohort6_heart_mid_fast  ATGAACTTGGCACA 7_146_cohort6_gut_mid_fast    CATTGGTGGTGTCC 7_146_cohort6_brain_mid_fast  CCAGCTTAGGAAGA 11_146_cohort8_testis_young_  ATGCAAGCGATTCC 11_146_cohort8_skin_young_    CCGGTGTGCGCTAA 11_146_cohort8_spleen_young_  ACGAGTATGTATGC 11_146_cohort8_liver_young_   ACGAGAGTGCTGAG 11_146_cohort8_kidney_young_  TCTGCTGCAGAGCG 11_146_cohort8_heart_young_   TCCTATGGTAGGAG 11_146_cohort8_gut_young_     CAATAGCAACACCA 11_146_cohort8_brain_young_   CCTCATTCGTTGAG 6_146_cohort8_testis_young_   CCGCCTTCTAACAC 6_146_cohort8_skin_young_     TTCTAGGACGGCCG 6_146_cohort8_spleen_young_   CAACCATTGGCAAG 6_146_cohort8_liver_young_    CTCCTACTTAATGC 6_146_cohort8_kidney_young_   AACGCTCAATGTGG 6_146_cohort8_heart_young_    TATCTTCCACCTGA 6_146_cohort8_gut_young_      CCGCGGAAGGATAA 6_146_cohort8_brain_young_    AACTGCTACGCACC 7_137_cohort8_testis_young_   AACGCTTCCGGTCC 7_137_cohort8_skin_young_     TTCTTCTCCAGAAG 7_137_cohort8_spleen_young_   AATCCGCCTAATGG 7_137_cohort8_liver_young_    TTAACACCTCAAGG 7_137_cohort8_kidney_young_   CAGCATCGAGAGCG 7_137_cohort8_heart_young_    TCAGAATCCTCGCC 7_137_cohort8_gut_young_      AACTTACACGTTGG 7_137_cohort8_brain_young_    TTCCACCGATGTGG 9_147_cohort8_testis_young_   TTCCAAGGTTGACA 9_147_cohort8_skin_young_     TATCATATGAGTCG 9_147_cohort8_spleen_young_   TCACTGAGTGCTGA 9_147_cohort8_liver_young_    ATCTAGCTGCACGA 9_147_cohort8_kidney_young_   ACCGTCTTCCTTGC 9_147_cohort8_heart_young_    AATGACCACGACCG 9_147_cohort8_gut_young_      TAATCCATCAACGC 9_147_cohort8_brain_young_    TCCTAACAAGTGCG 8_147_cohort8_testis_young_   CCAGTGAGGTATAC 8_147_cohort8_skin_young_     CACGCATCAGTCAG 8_147_cohort8_spleen_young_   TACTACTATGTGAC 8_147_cohort8_liver_young_    CCAAGCTTAGTGCG 8_147_cohort8_kidney_young_   CAACGTGGACCTAC 8_147_cohort8_heart_young_    TTCCGAAGCCTAAG 8_147_cohort8_gut_young_      TTCATTGGAAGAGA 8_147_cohort8_brain_young_    AAGCCAGGTAAGGC 3_147_cohort8_testis_young_   CAGTTGCGAAGTGG 3_147_cohort8_skin_young_     CATGAGACGTCGCG 3_147_cohort8_spleen_young_   CTAGTCTTGGTAGG 3_147_cohort8_liver_young_    TTGGACATTCCGGC 3_147_cohort8_kidney_young_   CATCTACGCTACGC 3_147_cohort8_heart_young_    CAACAAGACCTAAG 3_147_cohort8_gut_young_      TCCTCCGATGCCAG 3_147_cohort8_brain_young_    ACACACGACATCGC 2_147_cohort8_testis_young_   ATAGCACTCACACG 2_147_cohort8_skin_young_     CTGTCACACAGTCA 2_147_cohort8_spleen_young_   CTGGCGTTGAGTCG 2_147_cohort8_liver_young_    CAGTGGCCTAAGCA 2_147_cohort8_kidney_young_   ATGAATCAGCCGCC 2_147_cohort8_heart_young_    CACTTGGACCATCC 2_147_cohort8_gut_young_      AACAAGAGGTGTGG 2_147_cohort8_brain_young_    CAACGCCATAGACC 1_147_cohort8_testis_young_   CAAGTTCAGAATGG 1_147_cohort8_skin_young_     TCCTCCTCTTGTGA 1_147_cohort8_spleen_young_   AACGGCTGAACGAC 1_147_cohort8_liver_young_    ACCTTCTCACGGCC 1_147_cohort8_kidney_young_   ATTCTTCAGGAAGA 1_147_cohort8_heart_young_    ACTTCCAGGCCTGA 1_147_cohort8_gut_young_      CTGTGTAGGATTAA 1_147_cohort8_brain_young_    AATGCAAGACAACC