GSM9737680: Liver, Ctrl, EtOH, rep3; Mus musculus; RNA-Seq
Total RNA was extracted from snap-frozen liver tissue by mechanical homogenization in RNA-Solv reagent (Omega Bio-tek) using a MiniG 1600 homogenizer (SPEX), followed by purification using the E.Z.N.A. Total RNA Kit II (Omega Bio-tek). RNA concentration and purity were assessed by NanoDrop spectrophotometry (A260/A280 ≥ 2.0), and RNA integrity was confirmed by Bioanalyzer (Agilent Technologies); only samples with an RNA integrity number (RIN) ≥ 7 were submitted for sequencing. Poly-A selection was performed prior to strand-specific library construction. Library preparation was carried out using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs) as per the manufacturer's protocol.