Unveiling the distinctive impact of radiation on cardiac ventricular regions and the right atrium using multi-omics approaches
Unveiling the distinctive impact of radiation on cardiac ventricular regions and the right atrium using multi-omics approaches
Ten weeks post RT, one control mouse, one irradiated at the base and one at the apex were killed by a regulated procedure. Hearts were immediately extracted, washed in Phosphate buffered saline (PBS) and processed to be embedded in wax.According to 10x protocol for Visium, 5 micrometer sections were collected. Female C57BL/6J aged 12 weeks (16-20gr; Envigo, UK) were housed in standard housing conditions for laboratory animals (12-hour light-dark cycle, controlled temperature of 19–22°C and humidity of 40–65%) and provided with a standard diet and water ad libitum.Animals were anaesthetised with 2% isoflurane and were irradiated with X-rays (220 kV,13mA) at a dose rate of 2.67 Gy/min on a small-animal radiation research platform (SARRP; Xstrahl Life Sciences, UK). Briefly, cone beam CT (60kV, 0.7mA) collected 360 projections over 1 minute and it was used to reconstruct the 3D image of the whole mouse to identify the heart. Muriplan software was adopted to contour the region of interest, either the base or the apex of the heart to deliver the radiation. A total dose of 16Gy was delivered to the specific substructure of the heart using a 3 × 9 mm collimator. Used 10X sample collection protocol, detailed in  CG000409-10x Genomics userguide Used 10X sample collection protocol, CG000409-10x Genomics