GSM9305740: spikein-NNUNN-input-rep2; Homo sapiens; RNA-Seq
RNAs were extracted from cells using Trizol reagent and polyA RNA were extracted using Dynabeads® mRNA DIRECT™ Kit for two rounds. Messenger RNAs were extracted with an additional ribosome RNA removal step using the RiboMinus™ Eukaryote Kit v2. Libraries were constructed using a customized protocol. For the experiments of DKC1 knockdown, internally barcoded RNA adapters, wo that the four input samples (2 sictrl reps and 2 siDKC1 reps) and the four pull-down samples can be pooled together right after the RNA ligation. For IVT RNA, the RNA was mixed with rice mRNA in 1:1 ratio and then proceeded with library construction using ELAP-seq.