GSM8682733: TJP1iEC_E; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol reagent (Thermo Fisher Scientific) and purified using the RNeasy Mini Kit (Qiagen). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP