m-AAV-tSUAG2
Ribo-seq was performed by Gene Denovo Biotechnology Co. (Guangzhou, China) as described previously.67 Briefly, cells were pretreated with a final concentration of 100 g/mL cycloheximide (CHX) (sigma) for 2 min. Pretreated cells were lysed with lysis buffer and treated with RNase I (NEB) and 6 L of DNase I (NEB) for 45 min at room temperature with gentle mixing. RFs were purified and used to generate deep sequencing libraries, which were sequenced using Illumina HiSeqTM X14.