RNA-Seq of Microcystis synthetic communities: WT bipartite co-culture  d14 R2
RNA was extracted from cell pellets of 10 mL sample volume at each time point using the TriZOL reagent. Residual genomic DNA was removed by DNAse digest. Library construction was done by the following steps: rRNA Removal; RNA Fragmentation; 1st Strand Synthesis; 2nd Strand Synthesis (with dUTP); End repair, Add A and Adaptor ligation; UDG Digest and PCR amplification; Library QC; Cyclization; Sequencing