GSM8682815: Evs from osteocyte-enriched subchondral bone; high stress area;3; Homo sapiens; miRNA-Seq
The purification of total exosome-derived RNA was carried out by the exoRNeasy Maxi Kit (Qiagen). A total amount of 3 μg total RNA per sample was used as input material for the small RNA library. Sequencing libraries were generated using NEBNext®Multiplex Small RNA Library Prep Set for Illumina®(NEB, USA.) following manufacturer's recommendations and index codes were added to attribute sequences to each sample