GSM8545842: IgG_4; Mus musculus; RNA-Seq
KrasIKKaL cell line was maintained in RPMI1640 medium (Gibco, Waltham, MA, USA), supplemented with 10% fetal bovine serum (FBS, Gibco), 1% antibiotic-antimycotic mixture (Gibco), and 100 mg/L normocin (InvivoGen, San Diego, CA, USA). 6-8 week-old male C57BL/6 mice were intratracheally injected with 5 ´ 10^6 of KrasIKKaL cells. RNA libraries were prepared using MGIEasy RNA Directional Library PrepKit.