Viral Surveillance Panel 5_S5
total nucleic acid extracts were processed with the Illumina RNA Prep with Enrichment, (L) Tagmentation kit for cDNA and double-stranded DNA synthesis, and libraries generation by on-bead tagmentation, which simultaneously fragments DNA and incorporates sequencing adapters. Indexed libraries were subsequently hybridized with the viral probe set for enrichment. After overnight incubation, probe-target hybrids were captured with streptavidin-coated magnetic beads, and amplified by post-capture PCR to generate enriched libraries.