GSM9463998: Low light 1 mM  nitrate replicate 3; Chromochloris zofingiensis; RNA-Seq
RNA extraction was performed using mechanical disruption of the frozen cell pellets in a Mini Bead Beater (Biospec Products) mixed with 0.5 mm glass beads in the presence of an extraction buffer consisting of phenol:chloroform (1:1, v/v). Subse-quently, RNA was purified using ISOLATE II RNA Plant Kit (Bioline) following man-ufacturer's instructions. Sequencing libraries were generated according to Illumina TruSeq Stranded mRNA protocol and sequenced on an Illumina NextSeq 500 sequencer producing approxi-mately 17 million 150 nt long reads per sample.