eDNA CO1 sequencing of Vespa velutina sample A107
The sequencing library was prepared from total environmental DNA (eDNA) extracted from the gut content of hornet samples using a commercial DNA extraction kit. The mitochondrial COI barcode region was amplified using specific primers following standard eDNA metabarcoding protocols. PCR amplification was performed under optimized conditions to minimize non-target amplification. The resulting PCR products were purified, quantified, pooled, and subsequently used to construct the sequencing library according to the manufacturer's instructions. The final libraries were sequenced on the DNBSEQ-G99 platform using paired-end 150 bp mode.