GSM8940073: Unedited_NPC, Input, rep1; Homo sapiens; ChIP-Seq
Cells were crosslinked in 1% formaldehyde for 10 minutes at room temperature and quenched with 125 mM glycine. Nuclei were isolated, and chromatin was sheared using a sonicator to 200–500 bp fragments. Chromatin was incubated overnight with antibodies against H3K4me2, H3K4me3, or with no antibody for input controls, and immunoprecipitated using Protein A/G beads. ChIP and input DNA were processed using the NEBNext Ultra II DNA Library Prep Kit for Illumina following the manufacturer's protocol. Library size distribution was confirmed by Bioanalyzer and quantified using Qubit.