GSM8683857: HT7_rep1; Oryctolagus cuniculus; RNA-Seq
After collection, lungs were immediately placed in RNA Later (Sigma Aldrich, USA) and stored at -20°C. Samples were homogenized in QIAzol® Lysis Reagent. Total RNA was extracted with the miRNeasy Mini Kit protocol (QIAGEN, Germany), using an automated method (QIAcube: QIAGEN, Germany), adapted to include DNase I treatment. Libraries for massive parallel RNA sequencing (RNA-seq) were prepared with the Truseq Stranded mRNA Library kit (Illumina) and sequenced with an Illumina NovaSeq 6000 platform, which generated at least 20 million reads/sample (100x2 pb PE)