GSM9752508: Con (C57BL/6J) mouse hippocampus, 6 weeks, biological rep1; Mus musculus; RNA-Seq
Hippocampal tissues were dissected. Total RNA was extracted using MJzol Animal RNA Isolation Kit (Majorivd) and purified with RNAClean XP Kit (Beckman Coulter) and RNase-Free DNase Set (QIAGEN). RNA integrity was assessed by Agilent 2100 Bioanalyzer. mRNA was enriched by oligo-dT beads. mRNA-seq libraries were prepared using Dual-mode mRNA Library Prep Kit (YEASen). Enriched mRNA was fragmented, then first-strand and second-strand cDNA were synthesized. After end repair, A-tailing, adapter ligation, and PCR amplification, libraries were quality-controlled by Qubit 2.0 and Agilent 4200 TapeStation.