Whole genome sequencing of Staphylococcus phage
Library preparation was performed using the QIASeq FX DNA Library Preparation Kit (Cat# 180475) following the manufacturer's instructions. Briefly, 50 ng of Qubit-quantified DNA was enzymatically fragmented, end-repaired, and A-tailed in a one-tube reaction using the FX Enzyme Mix. The end-repaired fragments were ligated with index-incorporated Illumina adapters. The library was enriched by 6 cycles of Indexing-PCR (98C for 2 min, followed by 98C for 20 s, 60C for 30 s, 72C for 30 s, and a final extension at 72C for 1 min). The amplified library was purified using HighPrep beads (Magbio, #AC-60050) and assessed for quality. Quantification was done using a Qubit fluorometer, and fragment size distribution was analyzed on an Agilent 2200 TapeStation, showing a peak size of ~444 bp.