Feammox enrichment 1000
The V3/V4 region was amplified using the primers 341F (CCTAYGGGRBGCASCAG) and 806R (GGACTACNNNGGGTATCTAAT). The amplicons were purified, quantified, pooled, and then sequenced on the NovaSeq 6000 platform (Illumina, USA), in 250 pair-end (PE) mode. Quantification of the amplicons was performed on the Qubit 4 fluorimeter (Invitrogen, USA).