MDA-231-AdM-1834, MDA-231-LM2-4175, MDA-231-BoM-1833, MDA-231-BrM2-831, MDA-231-TGL, Calu-1, WM3211, WM4324, co-processed cultured human cancer cell lines used for EasySci single-cell RNA sequencing
Nuclei isolated from cultured human cancer cell lines were processed using the EasySci-RNA protocol. Reverse transcription, ligation-based barcoding, and indexed PCR amplification generated single-cell transcriptome libraries, sequenced on an Illumina NextSeq 1000 (paired-end 100 bp; dual 10 bp index reads).