GSM9853366: HEPM cells, ARHGAP29 siRNA, biological replicate 3; Homo sapiens; RNA-Seq
Total RNA was extracted from HEPM cells using the E.Z.N.A. Total RNA Kit (Omega Bio-tek). RNA quantity and purity were assessed before library preparation, and RNA integrity was evaluated using an Agilent 2100 Bioanalyzer. Ribosomal RNA was depleted from total RNA. Strand-specific libraries were prepared using the TruSeq Stranded Total RNA Library Prep Kit. RNA was fragmented, reverse-transcribed using random primers, converted to dUTP-containing double-stranded cDNA, adapter-ligated, size-selected and PCR-amplified. Qualified libraries were sequenced on an Illumina platform using a 2 x 150-bp paired-end strategy.