GSM9206277: D0-KO-2; Homo sapiens; RNA-Seq
RNA was extracted by standard Trizol extraction. For cardiomyocytes, RNA was polyA-enriched, followed by library preparation with Illumina stranded mRNA kits. For 293T experiments, polyA-enriched RNA was isolated with NEBNext® High Input Poly(A) mRNA Isolation kit, followed by library preparation utilizing the same protocol as seCLIP (Blue SM et al, Nature Protocols. 2022 PMID 35322209), based off of a published RNA-seq approach (Shishkin AA, Nature Methods. 2015 PMID 25730492).