H3K27ac-3
Porcine intestinal epithelial cells (IPEC-J2) were treated with ochratoxin A (OTA) or vehicle control for 24 hours with final concentration of 20M, RNA-seq, ATAC-seq, and CUT&Tag (H3K27ac) were performed to assess gene expression, chromatin accessibility, and histone modifications. Differentially expressed genes and epigenetic changes were identified, and their overlap was analyzed to uncover regulatory pathways. Super-enhancer and transcription factor motif analyses were conducted to further explore gene regulation.