GSM8720236: GGI-T2-RT1; Cynara cardunculus; RNA-Seq
With three biological replications, we extracted the heart tissue of artichoke on sampling day, wrapped in aluminum foil, frozen in liquid nitrogen, and stored at -80°C. Sequencing sampling was done at d7 and d14 for the reason of visible and physiological changes in artichoke quality. Total RNA from heart tissues was isolated using NucleoSpin RNA Plant (Machery- Nagel, PA, USA) and Fungi kit with additional DNase (PureLink™ DNase Set, Invitrogen™, CA, USA) at different time points. Quality & concentration (300 ng/mL) of RNA was checked using ND1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA).