GSM8506641: ATAC-seq_lhys_ZT24_1; Glycine max; ATAC-seq
Approximately 0.5 g of soybean mature leaves were used in each experiment. About 1 to 2g of the sample was ground into a fine powder in liquid nitrogen. For ATAC-seq, 50 k Isolated soybean nuclei were prepared for chromatin digestion by Tn5 transposase (VAHTS; cat. no. TD501) for 30 min at 37 °C. Digested chromatin was purified using a 2xCTAB followed by PCR amplification, product purification (vazyme, N411) and paired- end sequencing (2 ×150 bp) using an Illumina Hiseq X-Ten.