GSM8918983: IMR32_Dyngo4a_24h_R1; Homo sapiens; RNA-Seq
RNA isolation was performed using the miRNeasy kit from QIAGEN including an on-column deoxyribonuclease treatment, following manufacturer's instructions. total of 200ng of RNA was used as input for the library preparation with the QuantSeq 3′ mRNA library prep kit (Lexogen, Vienna, Austria) with umi barcoding according to the manufacturer's protocol. Sequencing of the RNA-seq libraries was conducted on the Illumina NextSeq 2000 platform using the NextSeq 2000 P2 Reagents 100 Cycles single-end kit (Illumina #20100981).