Amplicon seq of bird feces
Library was prepared according to the Illumina 16S Metagenomic Sequencing Library Preparation manual protocol (Illumina, CA, USA) with Q5 High-Fidelity DNA Polymerase (New England Biolabs, USA). The V3-V4 hypervariable region was amplified using primers Bakt_341F (5-CCTACGGGNGGCWGCAG-3) and Bakt_805R (5'-GACTACHVGGGTATCTAATCC-3').