GSM9822002: moDCs_LPS_CTLA4Ig_1; Homo sapiens; RNA-Seq
Total RNA from treated DCs was isolated using the PureLink RNA mini kit (catalog no. 12183018A, Invitrogen) according to the manufacturer's instructions. RNA quantity was assessed by NanoDrop spectrophotometry, and RNA integrity was evaluated using the Agilent Bioanalyzer RNA Nano Kit. 1ug of total RNA was used for the construction of sequencing libraries. RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina according to the manufacturer's instructions.100-bp single-end reads were generated per sample on an Illumina NovaSeq 6000 instrument.