16S rRNA sequencing of urethra: adolescent males
DNA extracted from urethral swabs, PCR amplification of the V4 region of the bacterial 16S rRNA gene, Primers: 515F/806R, Barcoded amplicons pooled, Ampure XP bead cleanup, Illumina MiSeq, paired-end 150 bp sequencing (MiSeq v2 chemistry)