GSM9535614: E85_rhesus_macaque_rep1, bulk ATAC-seq; Macaca mulatta; ATAC-seq
Aliquot 100,000 cells in a 1.5 ml microcentrifuge tube.Spin 300g for 3' at 4°C using swinging-bucket rotor. Carefully remove supernatant using P200 and P20 pipettes and add 100 μl cold Lysis Buffer. Gently pipette 3 times. Incubate cells on ice for 2' to 3'. Add 1 ml chilled Wash Buffer to lysed cells. Do not pipette. Spin 500g for 5' at 4°C using swinging-bucket rotor. OMNI ATAC Protocol