Recombinant HCV genome sequencing
Total RNA was extracted from human serum samples positive for Hepatitis C virus (HCV). The viral RNA was used for cDNA synthesis followed by library preparation for next-generation sequencing. Sequencing libraries were prepared to generate paired-end reads covering the viral genome. Multiple sequencing runs were performed for each sample to obtain complete genome coverage. The generated raw sequencing data were used for downstream analysis, including genome assembly, genotyping, and identification of recombinant regions.