Pacific Ocean metagenome: CLZ169
DNA libraries were prepared using the DNA Library Prep Kit with Fragmentation (Watchmaker Genomics) and Nextflex UDI sequencing adapters (Revvity) according to manufacturers instructions. The libraries were fragmented, amplified, analyzed via microcapillary electrophoresis on a LabChip GX Touch (Revvity), quantified by fluorometry on a Qubit instrument (LifeTechnologies), and pooled at equimolar concentrations.Pooled libraries were sequenced on two flowcells of the AVITI sequencer (Element Biosciences) run with paired-end 300 bp reads.