GSM9853876: ULA_NI_1; Mus musculus; RNA-Seq
For ultra-low attachment samples, dead cells were removed prior to extraction. Total RNA was isolated using the Direct-zol RNA Miniprep kit with TriReagent following the manufacturer's protocol. Poly-A mRNA was purified using poly-T oligo-attached magnetic beads, fragmented, and reverse transcribed to cDNA, which was end-repaired, adapter-ligated, amplified, and indexed to generate final libraries.