RNA-seq of tree shrew
Poly(A)+ mRNA (eukaryotes) or rRNA-depleted RNA (prokaryotes) was isolated from 12 g DNase-treated total RNA. mRNA was fragmented (150308 nt), reverse-transcribed into first-strand cDNA using random hexamers and SuperScript II, then converted to double-stranded cDNA. After AMPure XP bead purification, ends were repaired, dA-tailed, and Illumina adapters ligated. Final libraries were PCR-amplified and purified.