RNA-seq of Homo sapiens: transfected with plasmid encoding NOBOX c.1672_1675del CTCT, p.Phe559Cysfs*23 mutant
Total RNA was extracted from HeLa cells transfected with mutant NOBOX (MUT11) plasmid. mRNA enrichment was performed using oligo-dT beads, followed by library construction and paired-end sequencing on the BGISEQ-500 platform. The goal was to evaluate the transcriptomic changes induced by the NOBOX c.1672_1675del CTCT, p.Phe559Cysfs*23 mutation.