Sequence Reads Pig Gut
The community genomic DNA extracted from digesta samples of the cecum of each pig (Table S2) in groups 1 to 3 was sequenced using Illumina and Oxford Nanopore Technologies (ONP). The genomic DNA was converted into a Nanopore library with the SQK-LSK109TM kits. The library was sequenced on a SpotON R9.4.1 FLO-MIN106 flowcell for 72h, using a GridIONx5TM sequencer (Oxford Nanopore, Oxford, UK). Basecalling was performed with Guppy 5.1.13 (Oxford Nanopore, Oxford, UK) using the High Accuracy Mode. Reads were trimmed of adaptors with Porechop 0.2.3 (https://github.com/rrwick/Porechop).