FF110 (CRX2127990)
The product quality and concentration were assessed using both the Qubit Fluorometer and the Agilent 2100 Bioanalyzer. Libraries with DNA concentrations >=0.3 ng/uL were pooled for sequencing. The pooled libraries were denatured with NaOH and sequenced on an Illumina NovaSeq 6000 platform in paired-end 150 bp (PE150) mode using the SP Reagent Kit (500 cycles), yielding 2x150 bp reads that collectively covered all five target regions.