RNA Seq of BMMs: IGF2BP2_KO2_IL4
Myeloid-specific IGF2BP2 KO mice were generated by crossing B6.Cg-Igf2bp2tm1.1Thor/J mice (The Jackson Laboratory), which carry loxP sites flanking exons 4 and 5 of the Igf2bp2 gene, with 29P2-Lyz2tm1(cre)Ifo/J mice (The Jackson Laboratory). Control wild-type (WT) mice were littermates that did not carry floxed alleles and either expressed or lacked Cre recombinase. Bone marrow-derived macrophages (BMMs) were isolated from WT and IGF2BP2 KO mice and differentiated with M-CSF for six days. Cells were either left untreated (M0) or stimulated with IL-4 (20 ng/mL) for 8 hours to induce M2 polarization. RNA was extracted, and libraries were prepared using poly(A)-enriched mRNA. Sequencing was performed on an Illumina NextSeq 500 platform. All analyses were conducted in the R programming language. Differential gene expression was analyzed using DESeq2, identifying differentially expressed genes (DEGs, p < 0.05) when comparing IGF2BP2 KO vs. WT under both untreated and IL-4-treated conditions.