GSM8993031: WT_K27me3_pC_2; Arabidopsis thaliana; ChIP-Seq
Chromatin extracts were prepared from seedlings treated with 1% formaldehyde. The chromatin was sheared to the mean length of 500 bp by sonication, and proteins and DNA fragments were immunoprecipitated using antibodies against anti-GFP (Abcam, catalog no. ab290). The DNA cross-linked to immunoprecipitated proteins was reversed 2 ng of DNA from ChIP was used to ensure enough starting DNA for library construction. Two biological replicates were prepared and sequenced for each ChIP-seq experiment. The ChIP DNA was first tested by qRT-PCR and then used to prepare ChIP-seq libraries. End repair, adaptor ligation, and amplification were carried out using the KAPA DNA Library Prep kit (cat no. E7645) according to the manufacturer's protocol.