GSM8941797: WT_PBS_Biol_1; Mus musculus; RNA-Seq
RNA purification was performed according to Invitrogen's TrizolTM Regent protocol. Purified RNA sample concentrations were determined with a NanoDrop ND-1000 spectrophotometer for downstream cDNA synthesis. cDNA synthesis was carried out using the Maxima First Strand cDNA synthesis kit (Thermo Fisher Scientific, USA) according to manufacturer's instructions, with 372ng of RNA as the starting template. RNA quality and quantity were confirmed via NanoDrop measurement. All samples possessed an A260/280 purity ratio >1.7 and were sent to Genome Quebec (Montreal, Canada; RRID:SCR_017703) for poly-A enriched RNA-sequencing.