DZWZZFL3 (CRX2017535)
Total RNA was extracted from coral tissue using TRIzol (Registered) reagent according to the manufacturer's instructions. The RNA quality was then determined using a 5300 Bioanalyzer (Agilent) and quantified using the ND-2000 (NanoDrop Technologies). The high-quality RNA samples (OD260/280 = 1.8-2.2, OD260/230 >=2.0, 28S:18S >= 1.0, >1 mg, RIN >= 6.5) were used for cDNA library construction. After being quantified by Qubit 4.0, the sequencing library was performed on the NovaSeq X Plus platform (PE150) using the NovaSeq Reagent Kit.