GSM8683210: NPC, WT_3, rna-bis-seq; Homo sapiens; OTHER
Total RNA was extracted from each sample using the RNA mini kit (Qiagen, Germany), with quality assessed by gel electrophoresis and a Qubit. Watchmaker RNA Library Prep Kit was employed to deplete rRNA. Following RNase H and DNase I treatments, RNA purification was achieved using VAHTS RNA Clean Beads. The RNA was then subjected to bisulfite conversion with the EZ RNA Methylation Kit. After treatment, RNA was purified and reverse transcribed to cDNA. The cDNA was further purified and amplified using the Equinox Uracil Tolerant PCR Master Mix, with 15 cycles. Quantification of the libraries was performed using a Qubit fluorometer, after which they were sequenced on NovaSeq 6000 platform.