GSM8602629: Colon Tumour AKPT Rep6; Mus musculus; RNA-Seq
Segments of colon from mice with the noted genetic mutations were homogenised and digested using a Mcllwain Tissue Chopper and the GentleMACS Octo Dissociator (Miltenyi Biotec, 130-096-427) in combination with the mouse tumour dissociation kit (Miltenyi Biotec, 130-096-730). Cells were then sorted using a FACSAria sorter (BD Biosciences) and DAPI (Invitrogen, D1306) to remove dead cells. Libraries were prepared using reagents from the 10x Chromium Single-Cell 3′ v3 Gel Bead Kit and Library (10x Genomics) according to the manufacturer's protocol, then analysed using the Bioanalyzer High Sensitivity DNA Kit (Agilent Technologies).