GSM9347615: 4_Acute_preAC; Homo sapiens; RNA-Seq
Tissues were placed in tissue collection buffer immediately after collection and snap frozen in dry ice. For nuclei isolation, tissues were thawed in 500 μL homogenization buffer and incubated at room temperature with gentle rotation for 5 minutes. Samples were then transferred to ice to cool rapidly, then were homogenized to harvest the nuclei. Nuclei concentration was determined using the Countess II Automated Cell Counter (Thermo Fisher Scientific) with 0.4% Trypan Blue staining. snRNA libraries were constructed according to the manufacturer's instructions (Chromium™ Single Cell 3' Kit v3.1 protocol, 10x Genomics).