GSM9821666: IL2S5i 2; Mus musculus; RNA-Seq
At day 7, RNA was isolated from naïve or expanded CD8+ T cells using miRNeasy Mini Kit (QIAGEN). Poly-A mRNA enrichment was conducted using Dynabeads Oligo(dT) (ThermoFisher). Library preparation was carried out with the KAPA RNA Hyperprep Kit (Roche).