GSM9535603: Kidder_FLEX_library; Mus musculus; RNA-Seq
Single-cell suspensions were prepared from cultured ESC populations and processed according to the 10x Genomics Chromium Single Cell Gene Expression Flex protocol. Single-cell gene expression libraries were generated using the 10x Genomics Chromium Single Cell Gene Expression Flex platform with the Chromium Mouse Transcriptome Probe Set v1.0.1 (mm10-2020-A). Cells were encapsulated into gel bead-in-emulsions, where polyadenylated transcripts were captured and reverse transcribed to produce barcoded cDNA. Following cDNA amplification and cleanup, sequencing libraries were constructed according to the manufacturer's instructions.