GSM8941453: MC38_220822_64 Obese SDX; Mus musculus; RNA-Seq
Total RNA from MC38 tumors was extracted using Qiagen RNeasy Plus Universal mini kit following manufacturer's instructions (Qiagen). The RNA samples were quantified using Qubit 2.0 Fluorometer (Life Technologies) and RNA integrity was checked using TapeStation (Agilent Technologies). The RNA sequencing library was prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina using manufacturer's instructions (New England Biolabs), validated on TapeStation (Agilent Technologies), and quantified by Qubit 2.0 Fluorometer (Invitrogen) and quantitative PCR (KAPA Biosystems).