PB_HiFiseq of Fla
HMW DNA of 'Flava' was isolated from 1.5 gram material with a NucleoBond HMW DNA kit (Macherey Nagel). Quality was assessed with a FEMTOpulse device (Agilent) and quantity measured by fluorometry Quantus (Promega). A PacBio HiFi library was prepared according to the manual "Procedure & Checklist - Preparing HiFi SMRTbell Libraries using SMRTbell Express Template Prep Kit 2.0" with initial DNA fragmentation by g-Tubes (Covaris) and final library size binning by SageELF (Sage Science). Size distribution was again controlled by FEMTOpulse (Agilent). Size-selected libraries were sequenced on a Sequel II device at Max Planck Genome-centre Cologne (MP-GC) with Binding kit 2.0 and Sequel II Sequencing Kit 2.0 for 30 h.