WR10 (CRX2109998)
The standard protocol supplied by Oxford Nanopore Technologies (ONT) guided the execution of the experimental procedure. This protocol encompasses key steps such as sample quality assessment, library preparation, library quality verification, and library sequencing. High-quality genomic DNA was extracted and subsequently assessed for purity, concentration, and integrity using three methods: Nanodrop (NanoDrop Technologies, Wilmington, DE, USA), Qubit 3.0 Fluorometer (Life Technologies, Carlsbad, CA, USA), and 0.35% agarose gel electrophoresis. Large-fragment DNA was then recovered using the BluePippin automated nucleic acid recovery system. A sequencing library was constructed following the instructions of the SQK-LSK109 ligation kit.Subsequently, base correction and trimming of low-quality regions were performed on the sequencing reads with the correct tool from NECAT (v0.01) (https://github.com/xiaochuanle/NECAT/releases).