GSM8486741: BMSC, 1%O2, hypoxia 1; Rattus norvegicus; miRNA-Seq
total RNA was extracted from extracellular vesicles derived from BMSCs miRNA was isolated from the exosomal total RNA using the TaqMan ABC miRNA Purification Kit The small RNA libraries were constructed with GenSeq® Small RNA Library Prep Kit (GenSeq, Inc.) by following the manufacturer's instructions. Briefly, 3' and 5' adaptors were successively ligated to RNA samples. Then, the adaptor-ligated RNA was reversely transcribed into cDNA, followed by PCR amplification. After amplification, the cDNA libraries were size-selected for miRNA fractions before sequencing and then sequenced in a HiSeq platform (Illumina).