scRNA-seq NAT10 cKO-1 (CRX1466616)
cells were loaded into microfluidic channels, and a cDNA library was generated using 10x Genomics droplet-based sequencing platform. Single-cell transcript amplification and library preparation were performed using a Single Cell  Library Gel Bead Kit V2. Sequencing was performed on an Illumina NovaSeq 6000 sequencer with a sequencing depth of at least 100,000 reads per cell and 150-bp paired-end reads.