GSM8457428: bone marrow organoid day 16; Homo sapiens; RNA-Seq
Ten organoids were collected into a 15 mL Falcon tube and centrifuged at 100 × g for 5 minutes at room temperature. After discarding the supernatant, the organoids were washed twice with 5 mL of MACS buffer. Following the washes, the organoids were incubated at 37ºC with collagenase to facilitate tissue dissociation. The dissociated organoids were then resuspended in 5 mL of PBS and filtered through a 40 µm cell strainer. The resulting single-cell suspension was subsequently used for single-cell library construction. 10X Genomics NEXT GEM Kit V2, 10X genomics Library Construction kit