GSM9308436: 24 month, replicate 3; Mus musculus; RNA-Seq
RNA was isolated with Trizol followed by RNeasy MinElute Cleanup Kit (Qiagen). An on-column DNase digestion step was included. Cell content was used as direct input for cDNA generation and 500ug of cDNA was used as input for library generation. RNA libraries were prepared with an Illumina Stranded mRNA-Seq Library Prep kit following the manufacturer's protocol.