The bile acid CoA ligase, FATP5, is necessary for NAT synthesis in the liver
The bile acid CoA ligase, FATP5, is necessary for NAT synthesis in the liver
Livers of WT and FAAH-S268D mice that were fed for 3 days a HFD were used for RNA sequencing.  RNA was isolated and purified with RNeasy kit (Quiagen) Total RNA sequencing libraries were prepared using the Illumina TruSeq Stranded total RNA Gold protocol (Illumina). The total RNA was depleted of rRNA by RiboZero beads, fragmented, and cDNA was synthesized using SuperScript III Reverse Transcriptase (Thermo Fisher Scientific). cDNA was adenylated to prime for adapter ligation and after a clean-up using AMPure beads (Beckman coulter), DNA fragments were amplified using PCR followed by a final clean-up.