GSM9206914: F/F rep1; Mus musculus; RNA-Seq
Separated epidermal layer was washed with PBS and homogenized. Total RNA was isolated with TRIzol reagent and purified using the RNeasy Mini Kit. RNA sequencing libraries were prepared using the QIAseq UPX 3' Transcriptome Kit (#333088, QIAGEN). Subsequently, the libraries were adapted for the MGI sequencing platform with the MGIEasy Universal Library Conversion Kit (App-A) (#1000004155, MGI).