Ribo-seq of C.elegans culture environment
After extracting the total DNA of the sample, specific primers with Barcode are synthesized according to the full-length primer sequence, PCR amplification is performed, and the products are purified, quantified and normalized to form a sequencing library (SMRT Bell). The data of the PacBio Sequel II was in bam format, and the CCS file was exported through the smrtlink analysis software, and the data of different samples were identified according to the Barcode sequence and converted into fastq format data.