GSM9463110: Sample9,HC,scRNAseq; Homo sapiens; RNA-Seq
For peripheral blood mononuclear cells (PBMCs) isolation, 10 mL of heparinized blood was diluted 1:1 with PBS, gently layered over an equal volume of lymphocyte separation medium (Nacalai tesque, Japan), and centrifuged at 740 × g for 30 minutes; the mononuclear cell layer was then harvested. Isolated PBMCs were freshly subjected to the single-cell RNA sequencing, using the BD Rhapsody platform (BD Biosciences). Cell capture and library preparation were performed with the BD Rhapsody™ Human Immune Response Targeted Panel including 397 immune related genes following the manufacturer's instructions, and libraries were sequenced on an MGI DNBSEQ-G400RS.