RNA-seq of fish gonad by photoperid
Gonads were frozen with liquid nitrogen and pulverized in a grinding bowl, and total RNA was isolated using the Trizol Reagent (Invitrogen Life Technologies, Carlsbad, CA, USA). Three replicates were performed for each treatment group. The integrity of the extracted RNA was monitored using 1% agarose gels. The purity and concentration of RNA samples were assessed using a NanoDrop spectrophotometer (Thermo Scientific, Shanghai, China). Transcriptome sequencing was performed by Next-Generation Sequencing (NGS) technology using the Illumina Truseq 2000 sequencing platform from Shanghai Personal Biotechnology Co., Ltd. Shanghai Biotechnology Corporation (Shanghai, China) performed the transcriptome sequencing of the target samples.