GSM8941465: sihnRNPL3; Homo sapiens; RNA-Seq
RNA extraction was performed forty-eight hours after siRNA transfection following the TRIzol™ Reagent protocol (Thermo Fisher Scientific). RNA was quantified using the NanoDrop system. Retrotranscription was performed following the protocol of SuperScript™ IV VILO™ Master Mix (Invitrogen). The total RNA was processed for RNA-sequencing analysis with the TruSeq Stranded Total RNA gold (Illumina) following the manufacturer's instruction and sequenced on the NovaSeq6000 (Illumina).