GSM9821346: EU1 adult day 12, SULF, rep 4; Caenorhabditis elegans; RNA-Seq
Worms were picked into 0.2 mL tubes containing 45 µL lysis buffer, 4 µL RNA-secure, 5 µL proteinase K and flash frozen with liquid nitrogen. Libraries were prepared using the KAPA mRNA HyperPrep kit (KK8580 from Kapa Biosystems) as per manufacture protocol except that the total volume was adjusted to 1/4 per reaction.