Acidobacteriota genome
Libraries for short-read sequencing were prepared using the Illumina DNA Prep Tagmentation kit with 500 ng DNA input and 5 cycles indexing PCR. Library quality was evaluated (Agilent 2100 Bioanalyzer) and sequenced at an Illumina NovaSeq using a NovaSeq 6000 SP v1 sequencing kit with 2x150 bp read length and a depth of 4.05.0 Mio reads per sample. The sequencing was demultiplexed (Illumina bcl2fastq, v2.19.0.316), quality-checked (Fastq, v0.20.1) and visualized (MultiQC, v1.7). Paired-end reads were quality-filtered (Fastp (5) v0.20.1), assembled (Unicycler (6) v0.4.8), quality-checked (CheckM (7) v1.0.18) and genomes were annotated using Bakta (8) (v1.5.1).