Non-adherent transposon mutant library of S. canis strain IMT49926 after double HUVEC infection under flow, third biological replicate
HUVECs were grown to confluency under flow conditions of 10 dyn/cm and then infected with the pooled non-adherent mutant library after the first infection for 2.5 hours at shear rates of 6 dyn/cm. Non-adherent bacteria were harvested by washing with PBS and DNA was extracted from the mutant libraries after overnight growth at 37 C in Todd Hewitt Broth.