RNA-Seq of mus musculus: adult female Liver
After the sample test is qualified, the library is constructed. The main process is as follows:(1) Enrichment of eukaryotic mRNA with magnetic beads with Oligo (dT);(2) mRNA was randomly interrupted by Fragmentation Buffer.(3) Using mRNA as template, the first cDNA chain and two strands were synthesized, and the cDNA was purified;(4) The purified double-stranded cDNA was then end-repaired, A-tail was added and sequencing joints were connected, and AMPure XP beads were used for fragment size selection;(5) cDNA library was obtained by PCR enrichment.