GSM9347746: WT-LPS-01; Mus musculus; RNA-Seq
Gently aspirate the cell culture medium and add 1 ml Trizol reagent (15596026, Invitrogen).Total RNA was extracted from neutrophil samples using Trizol reagent. The integrity of the obtained RNA was assessed using the Agilent 2100 Bioanalyzer (Agilent, CA, USA). libraries were constructed using the VAHTS Universal V10 RNA-seq Library Prep Kit (Premixed Version) following the manufacturer's instructions.