GSM9677978: 231spheres_adipose_20ug_mL_dECM_Replicate1; Homo sapiens; RNA-Seq
Total RNA was extracted from the cell lines using Quick RNA Miniprep Kit from Zymo Research (Irvine, CA, USA) and sent to Plasmidsaurus (Burlington, MA, USA) for RNA sequencing. The RNA sequencing workflow after RNA isolation included generation of cDNA using a poly(dT)VN primer.  Illumina sequencing and a 3' end counting approach was carried out. Molecular identifiers (UMIs) were used to duplicate, and unique dual indices (UDIs) to prevent index hopping. The read length is ~90bp and all of the reads align within the last ~400nt of the transcript.