GSM9308561: Batch2_WT4_tumor; Mus musculus; RNA-Seq
Briefly, the tumor was dissected from the mammary fat pad of the mouse after 21 days. The tumor was then minced in a collagenase-hyaluronidase in 5% FBS/DMEM (High glucose) and incubated at 37 C for 30 min at 200 RPM. Cells were washed and resuspended in cold DBPS and the supernatant was discarded after spinning. A 0.25% trypsin, EDGA, polyvinyl alcohol solution in DPBS was added and incubated for 1 min at 37 C in water bath. Samples were neutralized with 2% FBS/DPBS and Dispase solution was added and incubated for 5 min at 37C. ACK lysis solution was used and pellets were kept in cold 2% FBS.PBS and stored. Cell viability was assessed prior to sequencing. Library was performed according to the manufacturer's instructions (single cell 3' v3 protocol, 10X genomics) using the 10XGenomics Chromium Controller.