Triple transfection of HEK293 cells; AAV
Multiplexing performed according to instructions for the Single cell Multiplexing Kit (Cat. No. 633781) (BD Biosciences, Franklin Lakes, USA). Single-cell isolation, RNA capture and cDNA synthesis performed according to instructions for the BD Rhapsody Express (BD Biosciences, Franklin Lakes, USA). Library preparation performed according to The Whole Transcriptome Analysis (WTA) Amplification Kit (Cat num: 633801) (BD Biosciences, Franklin Lakes, USA). Paired-end mRNA sequencing and adapter demultiplexing was performed using the Illumina NovaSeq 6000 platform (Illumina, Inc., USA).