GSM9677403: human_CSF_SPMS_5; Homo sapiens; RNA-Seq
A minimum of 3 ml fresh CSF was centrifuged, and the supernatant was frozen separately. Fresh CSF was processed directly. To generate single-cell libraries, cell suspensions were loaded onto the Chromium Single Cell Controller with the Chromium Single Cell 5′ Library & Gel Bead Kit v.1.1/v2 (both from 10X Genomics) in adherence to the manufacturer's guidelines.The fresh PMS CSF samples were processed individually using the Single Cell 5' Library Kit with a single index. Single Cell 5' Library Kit (10x Genomics)