Spray-Induced Gene Silencing (SIGS) with dsRNA: Efficacy, stability, and processing in potato plant protection against Phytophthora infestans
Spray-Induced Gene Silencing (SIGS) with dsRNA: Efficacy, stability, and processing in potato plant protection against Phytophthora infestans
For P infestans, following a 12 hour incubation of dsRNA samples were collected by flash freezing micro eppendorf tubes. For plant samples, 48 hours post dsRNA spraying leaf samples were selected and flash frozen. Total RNA was extracted with the mirVana miRNA isolation kit.  Library preparation and sequencing was conducted by NGI Sweden, SciLifeLab using a NovaSeq 6000 SP-100 flowcell. 