GSM9308851: MIA PaCa-2/shCWC22, Dox(+), rep2; Homo sapiens; RNA-Seq
Total RNA was purified using NucleoSpin RNA Plus (740984; Takara bio, Shiga, Japan). Purified RNA was subjected to RNA-seq by Rhelixa Inc. (Tokyo, Japan). In brief, mRNA was isolated using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Ipswich, MA), and a cDNA library for sequencing was prepared using NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs). Finally, samples were subjected to sequencing at 150 bp x2 by Illumina NovaSeq X Plus (Illumina, San Diego, CA, USA).