GSM9498569: MCF-7_HiC_10pM E2 30min_rep2; Homo sapiens; Hi-C
The Arima High Coverage HiC Kit (Arima, A410110) was used as the manufacturer's protocol. In short, cells were treated as indicated, and fixed with 2% formaldehyde before quencing. Cells were counted using the BioRad TC20 cell counter and 0.4x10^6 cells were used per assay. Digestion was performed using the Arima HiC 2.0 RE cocktail mix, disgested ends were biotinylated, and proximity ligation was performed. Samples were confirmed to be >15% biotinylated. Paired-ed sequencing libraries were prepared using the Arima Library Prep Module (Arima, A101030) as the manufacturer's protocol. In short, biotinylated DNA was sheared to ~550 to 600 bp usinig the Corvis E220 sonicator. 200 ng of resulting material was subjected to end repair, dA tailing, and adapter ligation as the protocol. Resulting libraries were amplified for 12 cycles before sequencing.