GSM9462099: Genioglossus, skeletal muscle cells, con-1; Mus musculus; RNA-Seq
The 10x Genomics platform utilizes microfluidic technology to encapsulate beads with Cell Barcodes and cells in droplets, collect the droplets containing cells, and then lyse the cells in the droplets, allowing the mRNA in the cells to bind to the Cell Barcodes on the beads, forming Single Cell GEMs. Reverse transcription reaction is carried out in the droplets to construct a cDNA library, and the sample source of the target sequence is distinguished by the sample index on the library sequence. The 10x Genomics platform utilizes microfluidic technology to encapsulate beads with Cell Barcodes and cells in droplets, collect the droplets containing cells, and then lyse the cells in the droplets, allowing the mRNA in the cells to bind to the Cell Barcodes on the beads, forming Single Cell GEMs. Reverse transcription reaction is carried out in the droplets to construct a cDNA library, and the sample source of the target sequence is distinguished by the sample index on the library sequence. The flowchart of experimental library construction and sequencing includes: sample preparation, cell quality inspection, labeled cells (barcode/UMI tags), library construction, and sequencing on the machine