Illumina NovaSeq 6000 paired end sequencing of SAMD01605729
Genomic DNA of the Aofad3-HC strain was extracted using ISOPLANT (Nippon Gene, Tokyo, Japan). Library construction was performed by Macrogen Japan using the TruSeq DNA PCR-Free Library Prep Kit (Illumina). Sequencing was conducted on the Illumina NovaSeq X Plus platform, generating 150 bp paired-end reads, with approximately 4 Gb of data per sample. Raw data were delivered in FASTQ format.