GSM9464819: Normal diet-Normal liver, SOX21CKO, rep4; Mus musculus; RNA-Seq
Total RNA was extracted from mouse colon crypt tissue. RNA quality was assessed by NanoDrop 2000, agarose gel electrophoresis, and Agilent 2100 Bioanalyzer (RIN). Libraries were prepared using the Illumina TruSeq RNA Sample Prep Kit, including mRNA enrichment, fragmentation, cDNA synthesis, adapter ligation, and PCR amplification.