RNA-Seq of pig nasal cavity samples
Nasal swab samples were collected from pigs , and total RNA was extracted. Host RNA was removed using a Ribo-Zero kit to enrich microbial RNA. RNA libraries were prepared using the Illumina TruSeq RNA Sample Preparation Kit and sequenced on an Illumina NovaSeq 6000 platform with paired-end 150 bp reads, generating approximately 20 million reads per sample