16S rDNA amplicon of 2023sprN24L1 (CRX2174953)
Genomic DNA was isolated through lysozyme-SDS-phenol/chloroform protocols and amplified with uniquely indexed primers targeting the 16S and 18S ribosomal RNA (rRNA) gene regions.the V4 domain of bacterial 16S rRNA was targeted using universal primers 515F/806R,High-resolution sequencing was performed on an Illumina Nova6000 platform (317-bp paired-end)