GSM9206191: Tmprss2CreERT2, lsl-EYFP, replicate 2, ATACseq; Mus musculus; ATAC-seq
Transposase-accessible chromatin sequencing (ATAC-seq) was performed using standard protocol {Buenrostro, 2013}. For each sample, 5,000 viable cells were processed for nuclei isolation and transposase treatment. The digestions were carried out for 30 min at 37℃. The library preparation and next generation sequencing were performed by MSKCC genomics core facility. For each sample, 40-50 million paired-end reads were sequenced on Illunima NovaSeq platform.