GSM9206688: L1037_1, D7V, caudal; L1037_2, D7V, proximal; L1041_1, R0G, caudal; L1041, R0G, proximal; all T1-Diff media; Homo sapiens; RNA-Seq
Recellularized lung FFPE tissues were sectioned and dissociated into single-cell suspensions using the gentleMACS Octo Dissociator, followed by single-cell capture with the 10x Genomics Chromium X system. Single Cell Fixed RNA libraries were constructed using the Chromium Single Cell Gene Expression Flex kit for multiplex samples. The libraries were sequenced with ~200 million PE150 reads per sample on Illumina NovaSeq X Plus.