GSM8718673: scRNA-seq of Hindbrain; Homo sapiens; RNA-Seq
hPSCs and differentiated ectoderm cells were enzymatically dissociated in Accutase (Thermo Fisher) at 37 ºC. Day 14 neuron-containing populations were dissociated in papain (Worthington). All samples were run through a 40 µM filter prior to fixation for 24 hours at 4C and storage at -80C. Single cell gene expression was profiled using the Chromium Single Cell Gene Expression Flex kit (10x Genomics) according to the manufacturer's recommendations. A multiplexed experimental design was used in which 10,000 cells were targeted to be recovered per probe barcode, and 5000 cells per sample were targeted to be captured on Chip Q. Library preparation was confirmed using Agilent Bioanalyzer (High Sensitivity DNA kit), and libraries were sequenced on a NovaSeq 6000.