GSM8506408: IVC-1; Bos taurus; RNA-Seq
RNA extraction was performed using the PicoPureTM RNA isolation kit (ThermoFisher Scientific, Waltham, MA, USA, catalog # KIT0204) following the manufacturer's instructions except that spin columns from the RNeasy Micro Kit for RNase-free DNA (QIAGEN, Hilden, Germany) were used instead of the mini columns provided by the PicoPure kit. Library construction and sequencing were performed at the Interdisciplinary Center for Biotechnology Research Gene Expression Core (ICBR) at the University of Florida. Low input RNA library construction was performed with SMARTer SMART-Seq® Single Cell Kit (Takara Biosciences, San Jose, CA, USA) combined with Illumina Nextera DNA Library Preparation Kit (San Diego, CA, USA) following the user guide instructions. The cDNA synthesis was performed using SMartScribe II Reverse Transcriptase (Takara Biosciences). The Illumina Nextera DNA Sample Preparation Kit was used for the Illumina sequencing libraries according to the manufacturer's instructions. Quantification of the libraries was assessed by Bioanalyzer and qPCR (Kapa Biosystems, Wilmington, WA, USA).