Metagenome of marine bacteria from salmon farm
Sequencing libraries were generated by random shearing of DNA fragments. The smaller DNA fragments were end repaired, A-tailed and further ligated with Illumina adapter. The fragments with adapters were PCR amplified, size selected, and purified. The library was checked and quantified with Qubit and real-time PCR, and a bioanalyzer was used for size distribution detection. Quantified libraries were pooled and sequenced on a Illumina NovaSeq 6000 platform with paired-end 150 bp configuration.