GSM8455377: CD4+ T cells, HIV-1 WT, donor #2, rep 2; Homo sapiens; RNA-Seq
RNA Extraction was carried out using the RNeasy Mini Kit (QIAGEN, Hilden, Germany) following the manufacturer's instructions, additionally employing QIAshredder (QIAGEN) spin columns for improved homogenization. To prevent DNA contaminations, on-column DNase (QIAGEN) digestion was performed. RNA was eluted from the column twice, each time adding 35μl RNase-free water (QIAGEN) to the column membrane, incubating for 1 min, and centrifuging as indicated by the protocol. RNA concentration and purity were assessed with a NanoDrop (Thermo Fisher Scientific) spectrophotometer. RNA was handled at 4°C and stored either at -20°C (short-term) or -80°C (long-term). RNA libraries were prepared using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® following the manufacturer's protocol.