sugarcane rhizosphere ratoon crop 1st year, replicate 3
Amplicon library was prepared using Nextera XT Index Kit as per 16S Metagenomic sequencing protocol. Primers for amplification of bacterial specific region were designed and synthesized at Eurofins Genomics Lab. 16S rRNA was amplified, checked on 1.2% Agrose gel. QC passed amplicons with Illumina adapters were amplified, assessed and were loaded on MiSeq for cluster generation and sequencing.