GSM9531701: AF22, COMMD3-BMI1_multiplex, 3; Homo sapiens; RNA-Seq
RNA was extracted using Sepazol Super G RNA (Nakarai Tesque) by the phenol-chloroform method. Total RNA was reverse‑transcribed using the ReverTra Ace® qPCR RT Master Mix with gDNA Remover (Toyobo, FSQ‑301), following the manufacturer's instructions. Multiplex PCR was performed using KOD‑Multi & Epi‑ (Toyobo) under the following conditions: 94 °C for 2 min, followed by 40 cycles of 98 °C for 10 s and 65 °C for 30 s. Libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) following manufacturer's protocols.