Amplicon sequencing of arbuscular mycorrhizal fungi: rhizosphere soil of Trifolium repens
Environmental DNA was extracted from mineral pouches, rhizosphere soil, roots, and fungal inoculum associated with Trifolium repens grown under greenhouse conditions. A portion of the small subunit (18S) rRNA gene specific to arbuscular mycorrhizal fungi was PCR-amplified using the AMF-targeted primers WANDA (5-CAGCCGCGGTAATTCCAGCT-3) and AML2 (5-GAACCCAAACACTTTGGTTTCC-3). Amplicons were sequenced using paired-end Illumina technology to characterize arbuscular mycorrhizal fungal community composition.