18srRNA-seq of pigeon
Total DNA was extracted using the NucleoSpin DNA Insect Kit (Macherey-Nagel, Dren, Germany), following the manufacturers instructions. The 16S rDNA V4 region was amplified by polymerase chain reaction (PCR) using the primers 515F141-TCGTCGGCAGCGTCAGATG(?:-df|-bvt|-s3|-gd|-current|-dev)*\.live(?:-tst)?\.net(?:\:[0-9]*)?/([A-Fa-f0-9]*)(?:\/.*)?$</RegularExpressio