ddRAD-seq of Cotoneaster ser. Salicifolii sp.
total genomic DNA was extracted from the dried leaves using a modified cetyltrimethylammonium bromide (CTAB) method (Doyle & Doyle, 1987). Qualified DNA (Qubit 3.0) was sent for ddRADseq library construction following Peterson et al. (2012), in which the restriction enzymes EcoRI and MseI were used to digest the genomic DNA, specific barcodes were added to each sample and 1024 samples were pooled together to select 350550 bp size as one library template. After PCR amplification, each library was sequenced on an Illumina NovaSeq (Illumina) in PE 150 mode.