RNA-Seq of Dugesia japonica: red midge larvae fed, sample 1
Planarians which had been maintained on red midge larvae for over a year were fasted 8 days prior to being frozen in -80 C. One frozen D. japonica was homogenized per sample in TRIzol (Invitrogen) and total RNA was extracted following standard TRIzol Reagent Guide. Extracted RNA underwent DNase treatment using the TURBO DNase kit (Invitrogen) according to manufacturers instructions. Sequencing libraries preparation was performed by Admera Health (South Plainfield, NJ) using the NEBNext Ultra II Directional with PolyA Selection kit. RNA-sequencing was performed by Admera Health using an Illumina NovaSeq X plus platform. The prepared libraries were 150 base pair pair-end se-quences with a read depth of 40 million pair-end reads per sample.