RNA-Seq of Bombus terrestris CNS
Libraries were prepared using 1 g of total RNA with the Illumina TruSeq stranded RNA sample preparation Kit as described in (Ragionieri and Predel, 2020). Sequencing was done using an Illumina TruSeq PE Cluster Kit v3 and an Illumina TruSeq SBS Kit v3 - HS on an Illumina HiSeq 4000 sequencer with a paired-end. Raw data (FASTQ files format) were filtered by removing adapter sequences and low-quality reads using Trimmomatic 0.38 (Bolger et al., 2014) The resulting filtered RAW reads were submitted to NCBI (Sequence Read Archives: pending; BioProject: pending). Filtered reads were de novo assembled using Trinity v2.2.0 (Grabherr et al., 2011; Haas et al., 2013) with read normalization option.