GSM8940103: siGPNMB imHMVECs 1; Homo sapiens; RNA-Seq
Total RNA was isolated using the Qiagen RNeasy Mini Kit according to the manufacturer's instructions. On-column DNase I treatment was performed to eliminate potential genomic DNA contamination. RNA quality and integrity were assessed at the Vanderbilt Technologies for Advanced Genomics (VANTAGE) Core using a NanoDrop spectrophotometer and an Agilent Bioanalyzer (Agilent Technologies, USA). Samples with sufficient RNA concentration, an A260/280 ratio of ~2.0, and high RNA Integrity Number (RIN) were selected for sequencing. Poly(A)-enriched RNA-seq libraries were prepared and sequenced on the Illumina NovaSeq 6000 platform (Illumina, USA) using 150 bp paired-end reads, yielding approximately 60 million passing filter (PF) reads per sample.