GSM9737330: GSC387_3; Homo sapiens; miRNA-Seq
RNA was harvested using Trizol kit. 1ug of total RNA was used folthe construction of sequencing libraries. Total RNA was used as input material for the RNA sample preparations. Briefly, 3' and 5' adaptors were ligated to 3' and 5' end of small RNA, respectively. Then the first strand cDNA was synthesized after hybridization with reverse transcription primer. The double-stranded cDNA library was generated through PCR enrichment. After purification and size selection, libraries with insertions between18~40 bp were ready for sequencing.