WGS of Bacillus safensis: mangrove sediment
The genomic DNA (gDNA) was extracted from the bacterial isolate using the Qiagen DNeasy Blood & Tissue Kit following the manufacturers instructions. The quality and concentration of the extracted DNA were verified via fluorometric quantification to ensure high-integrity templates for downstream processing. Sequencing libraries were subsequently prepared using the Illumina DNA Prep protocol, employing bead-linked transposomes to simultaneously fragment the DNA and ligate sequencing adapters. The resulting libraries were sequenced on the Illumina MiSeq platform using a v3 reagent kit, generating 2  300 bp paired-end reads.