16S rRNA amplicon sequencing of human-associated metagenome: knee synovial fluid V4M6
Total DNA was extracted from human knee synovial fluid samples using a commercial DNA extraction kit following the manufacturers instructions. The V4 region of the 16S rRNA gene was amplified using universal primers. PCR products were purified, indexed, and prepared according to the Illumina amplicon library preparation protocol. Libraries were quantified, normalized, pooled, and sequenced on an Illumina platform using paired-end.