N6-1 (CRX2183351)
Total RNA was extracted from samples using the TRIzol Reagent. RNA concentration and purity was assessed using NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA). RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). Sequencing libraries were constructed from 1 ug of total RNA per sample using the Hieff NGS Ultima Dual-mode mRNA Library Prep Kit. The final library quality was sequenced on an Illumina HiSeq X Ten platform by Biomarker Technologies.