WGS of Leishmania infantum LU22 (canine isolate)
Total DNA was extracted using the DNeasy Blood & Tissue Kit (QIAGEN, Hilden Germany), following the manufacturer's instructions. All strains were identified as L. infantum by PCR-ITS1-RFLP using the HaeIII enzyme. DNA quality was assessed by determining the DNA Integrity Number (DIN) through the analysis of 20 ng of DNA on a TapeStation 4200 (Agilent). One microgram of genomic DNA was used to prepare a library for whole-genome sequencing on an automated platform, using the Illumina PCR-free "TruSeq DNA" library preparation kit, according to the manufacturer's instructions. After normalization and quality control, the libraries were sequenced on an Illumina HiSeqX5 platform (Illumina Inc., CA, USA) at the National Center for Human Genetics Research (CEA, Evry, France), generating 150 bp paired-end reads.