DuplexSeq using TwinStrand Human Mutagenesis panel performed on DNA extracted from peripheral blood mononuclear cells isolated from a peripheral blood sample collected from the transplant recipient several years post-transplant.
DNA was fragmented using sonication to an average size of 300 bp. A unique molecular identifier was attached to both strands of a DNA duplex on both ends. A panel of probes was used to isolate specific genomic regions by hybrid selection, which were then amplified by PCR.