GSM9498139: Ctrl2; Mus musculus; RNA-Seq
Tissue samples were collected 48 hrs post last TPA application. Skin samples were harvested in PBS, cut in small pieces using a scalpel and digested with 100 μg/ml LiberaseTM (Roche) and 100 μg/ml of DNase I (Sigma-Aldrich) for 1 hr at 37 °C. 30,000 cells per sample were processed for single-cell RNA sequencing on a Chromium X Instrument using the GEM-X Single Cell 5' Reagent Kit (10X Genomics, Pleasanton, CA) and a single-cell gel bead emulsion was generated. cDNA amplification and library preparation were performed according to the manufacturer's instructions.