InVitro_CD4T_Il4_KO_2 (CRX1855450)
Total RNA was extracted from CD4+ T cells following stimulation with PMA (phorbol 12-myristate 13-acetate) and ionomycin using TRIzol reagent (Life Technologies). The resulting RNA-seq libraries were constructed and subsequently sequenced by BGI Genomics on the DNBSEQ platform utilizing 50-bp single-end reads.