GSM9376573: Leukemia cells-Piezo1cko_rep3; Mus musculus; RNA-Seq
Bone marrow cells were extracted from 8 to 10 weeks old Piezo1cko and wild-type (WT) AML mice.LSCs were sorted from WT and Piezo1cko BM leukemia cells.Total RNA were extracted with Trizol and mRNA were isolated with Oligo dT beads. The RNA-seq library was prepared using our customized protocol based on Smart-seq2 as previously described. In brief, reverse transcription (RT) of total RNA were reverse transcribed with VN-anchored oligo-dT primer and TSO, the resulting full-length cDNA is preamplified with the ISPCR primer. Pre-amplified cDNA was fragmented by Tn5 enzyme, followed by library generation using TruePrep DNA Library Prep Kit V2 for illumine kit (Vazyme, Cat#TD501) and were pair-end sequenced on Illumina HiSeq X Ten sequencing platform.