Raw reads: Tsu-0 (ecotype_ID: 7373) DNA
The plant material from rosettes of multiple 21-days-old individuals was finely ground with mortar and pestle in liquid nitrogen, and high-molecular-weight (HMW) DNA extraction was carried out as published (Rabanal et al., 2022; doi: 10.1093/nar/gkac1115). HMW-DNA (120 ng/μl) was sheared with Covaris gTUBEs (PN 520079) in a Eppendorf Centrifuge 5424 at 4,800 rpm (soft) for 3 x 1 min. 5 µg of Tsu-0 wild-type DNA was used as input for HiFi SMRTbell library using SMRTbell Express Template Prep Kit 2.0 with the barcode bc1010 multiplexed with two other libraries. Prior to sequencing the final pooled libraries were size-selected on a BluePippin (SageScience) with 10 kb cutoff in a 0.75% DF Marker S1 High Pass 6-10 kb v3 gel cassette (Biozym). Final libraries were sequenced on a single SMRTcell with the Sequel II system (Pacific Biosciences) using the Binding Kit 2.2.