GSM8717994: BMP4_YS_human_EB_Day6; Homo sapiens; RNA-Seq
On Days 2, 4, 6, 8, 10, and 15, embryoids were dissociated into single cells using Accutase for 15-30 minutes at 37°C. The cells were collected in 1.5 mL Eppendorf tubes, treated with 10% BSA/DPBS, and centrifuged at 180 g for 3 minutes. The samples were then washed twice with 1% BSA/DPBS and filtered through a 40 μm cell strainer. Cell density and viability were assessed using a CountStar (IC1000). Libraries were prepared using Single Cell 3′Library & gel Bead kit v3 (10x Genomics, Cat# PN-1000268) according to the manufacturer's protocol for 10000 cell recovery.