Genome of methanococcoides sp. isolate DA23
A barcoded SQK-NBD114.96 DNA library was prepared according to the manufacturers protocol,except for minor modifications (Oxford Nanopore Technologies, Oxford, United Kingdom). The barcoded DNA library was loaded onto a primed FLO-PRO114M (R10.4.1) flow cell and sequenced on a PromethION P24 device, running MinKNOW Release 23.11.4. Signal data was basecalled and demultiplexed with Dorado basecall server v. 7.2.13 (Oxford Nanopore Technologies, Oxford, United Kingdom) using the super-accurate algorithm (dna_r10.4.1_e8.2_400bps_5khz_sup.cfg). The remaining adapters were trimmed with Porechop v. 0.2.4. Removal of Low-quality reads and generation of basic sequencing data statistics were obtained using Nanoq v. 0.10.0 [6].