GSM9021012: Niacin Treated Mouse - Slide 1; Mus musculus; OTHER
The spatial transcriptomics analysis was conducted using the Visium Spatial Gene Expression platform by 10x Genomics. Brain tissues, collected 40 days after tumor implantation, were frozen and sectioned into 10-μm slices, following the manufacturer's guidelines. Sections were placed on Visium slides and processed as per the user guide. Tissue optimization studies delineated an optimal permeabilization time of 12 mins. Brightfield images distinguishing tumor and non-tumor regions were captured using the EVOS FL Auto Imaging System. The subsequent library preparation steps were performed as per the user guide (Visium Spatial Gene Expression Reagent Kit; CG000239). The libraries were then sequenced on an Illumina NovaSeq 6000 system, employing a NovaSeq 200 Cycle S1 flow cell and specific read cycles for sequencing.