GSM8547203: J1.1 cells treated with Birinapant 15A; Homo sapiens; RNA-Seq
Cells were lysed and RNA was extracted using Qiagen RNeasy mini kit (cat#74104) RNA sample quality assessment was performed with the Fragment Analyzer (Agilent) and concentration measured with the Qubit 3.0 (Thermo). Sequencing was performed with the Nextseq 2000 P3 100 cycle flow cell (Illumina). NGS libraries were prepared with the Stranded mRNA library prep kit (Illumina) using 500 ng of RNA input.