RAD-seq of Larix occidentalis: foliage
DNA was extracted from silica-dried foliage using an optimized version of the PL2 extraction protocol in the Machery-Nagel NucleoSpin 96 Plant II Core Kit. DNA was normalized to 20 ng/L. RAD-seq libraries were prepared by Floragenex Inc. (OR, USA) using the Sbf1 restriction enzyme.