stool_shotgun_metagenomics
The metagenomic DNA libraries were constructed using genomic DNA isolated from specimens using Illumina DNA Prep kit according to the manufacturer protocol. The quality of all libraries was evaluated using an Agilent TapeStation 4150 with a DNA HS D1000 tapes. The shotgun sequencing was carried out on Illumina NextSeq550 paired-end 150 read length.