GSM9206179: Lung iNKT scRNA-seq — Sublibrary2_S76 (2023-03 run); Mus musculus; RNA-Seq
Male and female 10–12-week-old mice on a C57BL/6 background were infected intratracheally with 1000 EIU PR8 (H1N1) or left uninfected. Lung iNKT cells (Lin– TCRβ+ CD1d-tetramer+) from NKTWT (Vα14tg; TCRα–/–; CD4Cre) and NKTET-2 (Vα14tg; TCRα–/–; CD4Cre; ET-2) mice were sorted at baseline or 48 h post-infection. Single-cell libraries were prepared with Parse Biosciences Evercode WT v2 and sequenced on an Illumina NovaSeq X Plus. Two experiments were performed; each sample pooled two mice.