GSM8683245: Control_CD4_1; Mus musculus; OTHER
RNA was prepared using trizol. mRNA was oligo-dT selected using Dynabeads mRNA direct kit and quantified using the qubit high sensitivity RNA assay. Libraries were generated with a nanopore direct cDNA sequencing with native barcoding kit (SQK-DCS109 with EXP-NBD104 and EXP-NBD114, Oxford Nanopore) and sequenced on R9.4.1 (FLO-MIN106) flow cells on a MinION sequencer (Oxford Nanopore).