RNA-Seq of Panax notoginseng
At green ripe stage, fresh roots of three-year-old plants were collected, immediately frozen in liquid nitrogen, and stored at -80 C prior to transcriptome analysis. Three biological replicates were included for each treatment. Total RNA was extracted from taproots using the RNAprep Pure Plant Kit (Tiangen, Beijing, China). RNA quality and integrity were evaluated using NanoDrop 2000 Spectrophotometer and Agilent 2100 Bioanalyzer, respectively. Sequencing libraries were prepared and subjected to paired-end sequencing on the Illumina NovaSeq 6000 platform.