RNA-seq
MLE-12 mouse alveolar epithelial cells were treated under normoxia, hyperoxia (75% O2 for 48h), or with Wnt5A overexpression. Total RNA was extracted, rRNA was depleted using a probe-based method, and strand-specific cDNA libraries were constructed with dUTP incorporation. Libraries were sequenced on an Illumina NovaSeq 6000 system in 150-bp paired-end mode.