GSM9376406: bgl1∆bgl2∆ Cellobiose Rep 1; Rhodotorula toruloides; RNA-Seq
Cells were harvested by centrifugation and flash frozen using liquid nitrogen. RNA was isolated using the Zymo Quick-RNA Fungal/Bacterial Ki according to manufacturer's protocols. RNA was treated with DNaseI (NEB) at 37C for 10 minutes followed by RNA cleanup using the Monarch Spin RNA Cleanup (NEB). RNA integrity was checked via agarose gel and spot-checked via BioAnalyzer. RNA were prepared for sequencing using poly-A enrichment and standard Illumina protocols.