Gut-metagenome of Pig
Fecal samples were collected from all piglets prior to inoculation (on PND 21) and post inoculation (on PND 27 and 48) timepoints. Cecal digesta samples were collected on PND 48. Genomic DNA was extracted from approximately 250 mg of fecal and cecal samples using DNeasy PowerSoil kit according to manufacturers instructions. The Illumina 16S metagenomic sequencing library preparation protocol was used to prepare amplicon libraries targeting the V3-V4 region of the 16S rRNA gene. The pooled library was paired-end sequenced on an Illumina MiSeq platform using 2 x 300 cycles.