GSM8508176: RNA_MTX2_03-06_2; Caenorhabditis elegans; RNA-Seq
Total RNA was extracted from approximately 300 L4 stage worms using TRIzol® (Thermo Fisher Scientific, Waltham, MA) according to the manufacturer's protocol. RNA quantification and purity assessment were performed using a NanoDrop™ 2000 (Thermo Fisher Scientific) and the RNA quality assessment using a 2100 Bioanalyzer (Agilent Technologies). library(biomaRt) library(tximport) library(readr) library(DESeq2) library(reshape) library(ggplot2) library(RColorBrewer) library(pheatmap) library(ggrepel) library(factoextra)