Microbial characterization of anaerobic batch reactors with sanitary sewage used in the degradation of artificial sweeteners.
Genomic DNA from the samples was extracted with FastDNA SPIN Kit for Soil DNA Extraction (MP Biomedicals), according to the manufacturer's recommendation. Oligonucleotide sequencing and basic bioinformatics service for taxonomic identification were performed by NGS Solues Genmicas (Piracicaba, SP, Brazil). The primer sets used in the amplification of the 16S rRNA gene were: 341F -785R for the Bacteria Domain (Klindworth et al., 2013) and Ar915aF - Ar1386R (Kittelmann et al., 2013) for the Archaea Domain. The obtained sequences submitted assembly and filtering processes to remove low-quality bases (QPhred ?33) and identify and eliminate chimeras, using the DADA2 pipeline (CALLAHAN et al., 2016) in the R environment. The taxonomic classification of Amplicon Sequence Variants (ASVs) was performed for sequences with ?97% similarity, with taxonomic assignment based on version 138 of the SILVA database.