GSM8457401: Intermediate flowering, drought-stressed; Sorghum bicolor; RNA-Seq
All leaves were frozen in liquid nitrogen immediately after collecting and were then stored at minus 80 °C. Total RNA was separately isolated from frozen flag leaves samples (those collected from three different plants of each group when pollen shed began) using NucleoSpin® RNA Plant kit (MACHEREY-NAGEL). RNA quality and integrity were assessed using Quantus™ Fluorometer (Promega) prior to RNA-sequencing (at Macrogen Inc., Seoul, South Korea). The cDNA library preparation was done using a TrueSeq Stranded mRNA LT Sample prep kit.