Injured_hic_data_of_motor_cortex
Cells were crosslinked with 1% formaldehyde for 10 minutes at room temperature. Following quenching with glycine (125 mM), chromatin was isolated and digested with MboI restriction enzyme. DNA overhangs were filled with biotin-dATP and subjected to proximity ligation. After crosslink reversal and DNA purification, biotin-containing fragments were captured using streptavidin beads. Libraries were prepared from these fragments and sequenced on an Illumina HiSeq 2500 platform with paired-end 100-cycle reads, generating approximately 200 million read pairs per sample. Reads were mapped to the reference genome using Bowtie2, and valid interaction pairs were identified after removing PCR duplicates and filtering for mapping quality.