GSM9497456: FK-D5-04; Homo sapiens; RNA-Seq
RNA was extracted using Trizol/Phenol-Chloroform Method. RNA samples were quantified using Qubit 4 Fluorometer (Life Technologies, Carlsbad, CA, USA) and RNA integrity was checked using Agilent TapeStation 4200 (Agilent Technologies, Palo Alto, CA, USA). RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer's instructions (NEB, Ipswich, MA, USA).