GSM9307762: A2; Homo sapiens; RNA-Seq
hSSCs were seeded into 96 well plates at 3000 cells per well onto Active Wnt3a platform or Inactive Wnt3a platform with 100 μl hSSC basal medium and cultured for 24h to allow cells to attach. After 24h, media was replaced with osteogenic media and cells were cultured for 48h. RNA was extracted and purified using a commercial kit (Nucleospin RNA XS, Macherey-Nagel) according to the manufacturer's recommendation. 30 wells were pooled to obtain enough material and counted as 1 biological replicate (n=3 per group). Samples were sequenced using the NovaSeq6000 instrument (Illumina).