GSM8682912: siNT Replicate 3; Mus musculus; OTHER
Cells were collected in Trizol, RNA was extracted, and genomic DNA contamination removed using DNAse. RNA was then fragmented. 4sU-containing RNA was biotinylated, pulled down, and washed. RNA was then eluted and purified. 4sU-labeled RNA was used as input to the Illumina TruSeq stranded total RNA kit using the manufacturer's protocol.