16S (V4) metabarcoding analysis of skin of Cephalorhynchus eutropia and Lagenorhynchus australis
The V4 hypervariable region of the 16S rRNA gene was amplified from the extracted DNA using the barcoded dual-index primers. Preparation of libraries and paired end (2 x 150 bp) Illumina MiSeq sequencing was conducted at Argonne National Laboratory (Lemont, U.S.A.).