GSM9887502: RNA-seq GN, ctrl, FANS, IVE+day8, rep6; Mus musculus; RNA-Seq
Cerebella were removed and microdissected. For electroporated samples, electroporation efficiency was assessed under a stereomicroscope and mCherry+/NeuN+ nuclei were enriched using fluorescence activated nuclei sorting with a SH800S cell sorter (Sony). For FANS-RNA-seq, half the amount of purified RNA was treated with a NEBNext rRNA Depletion Kit (New England Biolabs) and libraries were prepared with the NEBNext® Ultra™ Directional RNA Library Prep Kit (#E7420). For sync-TRAP-seq, half the amount of immunopurified RNA was subjected to library preparation using the Trio RNA-seq kit with ribo-depletion (TECAN) according to the manufacturer's instructions.