GSM9752689: HD1; Homo sapiens; miRNA-Seq
Whole blood samples were centrifuged to obtain cell-free plasma. Plasma extracellular vesicles were isolated and used for downstream RNA extraction. EV-associated small RNAs were extracted from purified extracellular vesicles for small RNA library construction. Small-RNA sequencing libraries were prepared from EV-associated RNA. Adapters were ligated to RNA molecules, reverse transcription was performed, and cDNA products were amplified to generate sequencing libraries. Libraries were quantified and sequenced on an Illumina NovaSeq platform.