Spatial transcriptomics of adipocyte-specific ATGL-KO mice 24 h after cardiac ischemia.
Spatial transcriptomics of adipocyte-specific ATGL-KO mice 24 h after cardiac ischemia.
After mouse sacrifice hearts were excised and retrograde perfused with first 5ml Heparin/PBS solution followed by 1ml PBS. Perfused hearts were place in OCT, snap-frozen in pre-cooled isopentane (-30 to -40°C) and stored at -80°C until further use. 10µm thick tissue sections were cut in a cryostat at -20°C chamber temperature and -10°C object head temperature and placed at the 10X Visium Gene Expression slide. Prepared Visium slides were used as input for the Visium Spatial Gene Expression workflow  v1 (10X Genomics, Pleasanton, USA) according to the manufacturer's instructions. Tissue permeabilization was carried out for 12 minutes Library construction was performed to manufacturer's instructions, the Visium Spatial Gene Expression workflow (10X Genomics, Pleasanton, USA).