GSM8547321: C2_93_BL; Homo sapiens; RNA-Seq
PBMC samples were thawed, pelleted, and lysed in 350 uL of RLT with beta-mercaptoethanol. RNA was isolated using the RNeasy Mini kit (Qiagen). RNA quality was assessed with the Fragment Analyzer (Agilent) and its Standard Sensitivity RNA kit. Total RNA was normalized to 100 ng prior to random hexamer priming and libraries generated by the TruSeq Stranded Total RNA – Globin kit (Illumina). The resulting libraries were assessed on the Fragment Analyzer (Agilent) with the High Sense Large Fragment kit and quantified using a Qubit 3.0 fluorometer (Life Technologies)