Metagenomic sequencing of low quality wastewater irrgated clover
For produce samples, DNA was extracted from 1 ml of stomacher homogenate or enrichment culture for direct and enriched produce samples, respectively. Bacterial suspensions were centrifuged at 5000 rpm for 1 min to obtain bacterial pellets. Pellets were stored at -80C until genomic DNA extraction with the Exgene Cell SV mini kit (catalog no. 106-101; GeneAll, South Korea).