GSM9886424: H3K27ac ChIP-seq GN, ctrl, FANS, IVE+day4, rep1; Mus musculus; ChIP-Seq
Cerebella were removed and microdissected. For electroporated samples, electroporation efficiency was assessed under a stereomicroscope and mCherry+/NeuN+ nuclei were enriched using fluorescence activated nuclei sorting with a SH800S cell sorter (Sony). Lysates were sonicated, and protein-DNA complexes were isolated using the indicated antibodies. Brd4 ChIP-seq libraries were prepared by tagmentation and with the NEB Q5® Hot Start High-Fidelity 2X Master Mix (M0494S). H3K27ac ChIP-seq libraries were prepared with the NEBNext Ultra™ II DNA Library Prep Kit (#E7645).