GSM8455530: Sample_HPNE.963_CAM_v2; Homo sapiens; RNA-Seq
Cells were cultured 24 hours, washed 2x with 1mL of ice-cold PBS [Sera-Care 5460-0023], then lysed in 300μL QIA-zol [Qiagen#51-13-02]. RNA was extracted using Direct-zol RNA Miniprep Kit [Zymo Research#R2052] following the manufacturer's protocol. Library prep was completed following Tecan Universal Plus mRNA library Prep (Stranded mRNA Library), then sequenced on a NovaSeq S1-200 cycle (100PE) flow cell using XP 2-lane chemistry for independent lane loading.  A second sequencing run was used to provide supplemental reads for samples HPNE_297 & HPNE_415. A second set of experiments were combined as more patient samples were accrued. Groupings of samples were MiaPaCa2, Panc1, and HPNE-G12D.