Multi-Tissue WGBS Analysis in Mini Pigs: Body Size and Tissue-Specific Comparisons
Multi-Tissue WGBS Analysis in Mini Pigs: Body Size and Tissue-Specific Comparisons
To identify tissue-specific body size-related genes, approximately 10 samples representing 5 different tissues were collected from MiniPig individuals with varying body sizes. This comprehensive sampling aimed to capture the relationship between tissue expression patterns and body size variations. All experimental procedures were thoroughly reviewed and approved by the National Institute of Animal Science (NIAS) under approval number NIAS20181295. Genomic DNA was extracted from tissue samples using the QIAamp DNA Mini Kit (Qiagen, Cat. No. 51306) following the manufacturerâ€™s protocol. DNA concentration and purity were assessed, and only samples meeting quality standards were used for library preparation. Individual libraries were generated using the Accel-NGS Methyl-Seq DNA Library Kit (Swift BioSciences) following the manufacturer's protocol. The bisulfite-converted DNA fragments were repaired, adapter-ligated, and amplified to prepare the WGBS libraries.