non-depleted PBMCs, healthy
Plasma (cfDNA) and PBMC (buffy coat genomic DNA) isolated from human blood underwent double centrifugation. cfDNA was extracted with nRichDX Revolution cfDNA Max Kit. gDNA was extracted with NEB Monarch Genomic DNA Purification Kit. For each sample, replicate libraries were prepared with and without host depletion. Libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit with unique dual-index UMI adapters. Host depleted libraries targeted ~15x coverage, and non-depleted libraries targeted ~30x genome coverage.