GSM9533782: RA1-P; Homo sapiens; ncRNA-Seq
Total RNA was extracted using TRIzol Reagent (1 ml; Invitrogen; 15596018). Small RNAs of 15–50 nucleotides was selected. The RNA segment was fractionated using PAGE, then a 15- to 45-nucleotide stripe was selected and purified. Adapter ligation and library amplification were performed using the QIAseq® miRNA Library Kit (QIAGEN; 331505). The amplified libraries were sequenced on Next-Generation Sequencing (NGS) platform.