GSM9207301: WT Bd21-3 leaf developmental zone, protoplasted, replicate 1; Brachypodium distachyon; RNA-Seq
RNA extraction was performed with the RNeasy micro kit (Qiagen) following the manufacturer's instructions Extracted RNA was quality checked with an Agilent 2100 Bioanalyzer, NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) using NEBNext Multiplex Oligos for Illumina for sample indexing was used to prepare sequencing libraries