GSM9305442: Placental organoid knockdown 1; Homo sapiens; RNA-Seq
Placental organoids were pelleted and resuspended in trizol before being mechanically dissociated utilizing a tissue lyser. RNA was extracted by utilizing Invitrogen PureLink RNA Mini Kit. CT27 and CT29 cell lines were flash-frozen and the nuclei for both cell lines were collected using the 10X Chromium Nuclei Isolation Kit with RNAse inhibitor following the manufacturer's intructions. Bulk seq libraries were contructed using the Illumina Stranded mRNA Prep Kit. Single nuclei libraries were prepared using the 10X GEM-X Universal 3' Gene expression v4 4-plex according to manufacturer's instructions.