GSM8455194: Li_9w_ATAC_XistBxC_-+_XX_2; Mus musculus; ATAC-seq
Nuclei from liquid nitrogen frozen organs were isolated using the Nuclei Extraction Buffer (Miltenyi) according to manufacturer's instructions. For ATAC-sequencing, the integrity of isolated nuclei was assessed on the Countess™ II FL Automated Cell Counter equipped with the DAPI EVOS light cube. 50,000 nuclei were taken as input for Active Motif's ATAC-Seq kit and libraries were generated according to manufacturer's instructions. ATAC-seq library was generated using the Active Motif's ATAC-Seq kit.