RNA-Seq of Pseudomonas aeruginosa MB663
Total RNA was extracted from Pseudomonas aeruginosa strain MB663 cultured in 100% human serum under biofilm and planktonic growth conditions after 48 h incubation. RNA was stabilized with RNAlater, extracted using the RNeasy Kit (Qiagen), and quantified using a Qubit fluorometer. cDNA libraries were prepared using the ImProm-II Reverse Transcription System and sequenced for comparative transcriptomic analysis.