Metagenome of  rumen microbiota of goat fed fermented feed 3 of KFL in nanjing (CRX2117213)
DNA in the rumen content was extracted using a DNeasy PowerSoil Kit (Cat. No.12888, Qiagen, Valencia, CA, USA). The extracted total DNA was processed to construct metagenome shotgun sequencing libraries with insert sizes of approximately ~400 bp, using an Illumina TruSeq Nano DNA LT Library Preparation Kit (Illumina, USA). Each library was sequenced using an Illumina NovaSeq X Plus platform (Illumina, USA) with PE150 strategy at Personal Biotechnology Co., Ltd. (Shanghai, China).