GSM9462198: Adult rat central canal associated spinal cord cells, ex vivo expanded (Rat_3); Rattus norvegicus; RNA-Seq
Total RNA was isolated from central canal–associated spinal cord cell cultures using a column-based kit with on-column DNase digestion (Qiagen RNeasy Plus). RNA integrity was assessed by Bioanalyzer/TapeStation and quantified by Qubit Poly(A)+ RNA was enriched and strand-specific libraries were prepared using Illumina TruSeq Stranded mRNA reagents with unique sample barcodes. Libraries were sequenced on an Illumina NovaSeq 6000 (150 bp, single-end).