GSM9853412: Organoids_Control_ASO_OPC; Homo sapiens; OTHER
Both dead and viable cell populations were single-cell deposited into individual wells of a 96-well plate, having a PCR-compatible Proteinase K-based lysis buffer (10 mM Tris-HCl (pH 8.0), 50 mM KCl, 2.5 mM MgCl2, 0.45% NP-40, 0.45% Tween-20, Proteinase K (100 µg/mL)), followed by 55°C, 30 min incubation to disrupt both cell and nuclear membranes and then heat-inactivation of the Proteinase K at 95°C for 10 minutes. ASO Barcodes (DNA) from different wells were amplified for Illumina sequencing with the combination of cell barcodes unique for each well (Illumina DNA/RNA Unique Dual Indexes (UDI)