16S rRNA gene sequencing of soil DNA: sample FT59-20191210
Libraries for sequencing were prepared according to Illuminas 16S Metagenomic Sequencing Library Preparation guide, with the exception of using Qiagen HotStarTaq Plus polymerase for the first PCR (amplicon PCR). The template specific primers were (without the overhang adapter sequence): 515F-Y (5- GTGYCAGCMGCCGCGGTAA-3) and 926R (5- CCGYCAATTYMTTTRAGTTT-3).