Amplicon seq of great tit feces
Library was prepared according to the Illumina 16S Metagenomic Sequencing Library Preparation manual protocol (Illumina, CA, USA) with KAPA HiFi HotStart ReadyMix (Kapa Biosystems, MA, USA). The V3-V4 hypervariable region was amplified using primers Bakt_341F (5-CCTACGGGNGGCWGCAG-3) and Bakt_805R (5'-GACTACHVGGGTATCTAATCC-3').