GSM8653697: PN0456_0003; Homo sapiens; RNA-Seq
Total RNA was isolated using the Maxwell RSC simplyRNA Cells Kit (Promega) and a Maxwell RSC instrument (Promega) following the manufacturer's instructions. RNA quantity and purity were assessed using the Nanodrop (Thermo Fisher Scientific)and the quality was assessed using the Fragment Analyzer (Agilent Technologies). Libraries were prepared using KAPA RNA HyperPrep Kit with Poly A enrichment (Roche). Samples were sequenced on a NextSeq 2000 instrument (Illumina) to yield at least 40 million reads per sample, read length 2x50bp PE.