3_E1_a
cDNA Libraries preparation and sequencing Total RNA was rRNA depleted using Ribo-ZeroTM rRNA Removal Kit Bacteria (Illumina) and cDNA libraries were obtained following Illumina's recommendations. We started from 2 g of total RNA (RIN>9) libraries. Briefly, RNA ribosomal depleted was chemically fragmented prior to reverse transcription and cDNA generation. The cDNA fragments then went through an end repair process, the addition of a single 'A' base to the 3' end and then ligation of the adapters. Finally, the products were purified and enriched with PCR to create the indexed final double stranded cDNA library. The quality of the libraries was analyzed in TapeStation 4200, High Sensitivity assay