ATFGF-7WT1 (CRX1227556)
RNA-seq transcriptome libraries were prepared following TruSeqTM RNA sample preparation Kit from IlluminaSan Diego, CA, using  of total RNA. Shortly, messenger RNA was isolated with polyA selection by oligodT beads and fragmented using fragmentation buffer. cDNA synthesis, end repair, A-base addition and ligation of the Illumina-indexed adaptors were performed according to Illumina's protocol.1