GSM9376370: A child with ca6 hand-foot-mouth disease in the acute stage.; Homo sapiens; RNA-Seq
PBMCs were prepared by Ficoll density gradient centrifugation. The cell count and viability of the single-cell suspension were determined by an automatic cell counter, and the cell viability was greater than 85%. The live cells were washed and resuspended at a concentration of 700-1200 cells per µL. The generation of single-cell gel beads (GEM), reverse transcription, cDNA amplification, library construction and cDNA quality detection were carried out. After quality confirmation, sequencing was performed using the Illumina NovaSeq 6000 platform.