GSM8464757: OVCAR3CIS cells, siMMP3+CIS, rep2; Homo sapiens; RNA-Seq
Controls, including untreated cells and cells treated with only cisplatin (four biological replicates of each condition), were extracted with the Mirvana RNA Isolation Kit (Fisher, Grand Island, NY, USA). The integrity of the RNA samples was evaluated via an Agilent Bioanalyzer 2100, and 1.0 µg of high-quality RNA was used for the sequencing protocol. The RNA was enriched, and the library was prepared via GENEWIZ® Strand-specific RNA sequencing with rRNA depletion (GENEWIZ, Inc. South Plainfield, NJ, USA).