GSM8718781: Lipus-2; Rattus norvegicus; RNA-Seq
TRIzol Reagent (Thermo Fisher Scientific, Waltham, MA) First, total RNA is extracted from the tissue ( rat sciatic nerve), and mRNA is isolated using magnetic beads with Poly-T oligo. The isolated mRNA is fragmented into smaller pieces, followed by complementary DNA (cDNA) synthesis through reverse transcription. The cDNA ends are repaired, and sequencing adapters are ligated to both ends of the cDNA fragments. PCR amplification enriches the library for the desired fragments. The quality and quantity of the library are assessed using a Qubit Fluorometer and an Agilent Bioanalyzer 2100. Finally, the prepared library is sequenced using the Illumina NovaSeq 6000 platform