Cissus hexangularis chloroplast, complete genome (CRX2069884)
Genomic DNA was extracted using the DNeasy Plant Mini Kit from Qiagen (Germany) following the manufacturer's instructions. A sequencing library construction strategy with an average insert size of approximately 300 base pairs was used, and sequencing was performed on the Illumina HiSeq 2500 platform using a 150 base pair paired-end sequencing strategy. The raw reads were quality filtered using FASTP v0.20.0. The complete chloroplast genome was de novo assembled using GetOrganelle v1.7.5.