GSM8825876: CON2; Mus musculus; RNA-Seq
200 cells per sample were sorted in 3ul cell lysis buffer (final concentration: 2.5mM dNTP, 1U/ul RNase Inhibitor, 0.1% Triton X-100) and frozen immediately on dry ice after isolation. The reaction system is configured for reverse transcription. First-strand cDNA is synthesized with SMART-SeqII. The reaction system is configured for PCR amplification to process preamplification of cDNAs. Qualified amplified cDNAs use transposon-based method for further library construction. Single-stranded PCR products are produced via denaturation. The reaction system and program for circularization are subsequently configured and set up. Single-stranded cyclized products are produced, while uncyclized linear DNA molecules are digested.