GSM8940057: Patient1_Unedited_NPC biological replicate 3; Homo sapiens; RNA-Seq
Total RNA was isolated using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions, including on-column DNase digestion. Libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit (New England Biolabs) following poly(A) enrichment. Libraries were quantified, pooled, and sequenced on an Illumina platform.