Visium of homo sapiens: gestastional week 20 brain
Visium Spatial Gene Expression Analysis (10x Genomics) was performed according to manufacturers user guide and demonstrated protocol (Visium CytAssist Spatial Gene Expression for Fresh Frozen  Methanol Fixation, H&E Staining, Imaging & Destaining, CG000614, Rev A; Visium CytAssist Spatial Gene Expression Reagent Kits User Guide, CG000495, Rev D). Briefly, 10m-thick cryosections adjacent to the sections used for MERFISH analysis were adhered to Superfrost slides. Two samples were used for Visium analysis, FB080-O1, GW20 occipital cortex sample containing the V1 and V2, and FB121-F1, GW20 prefrontal cortex. FB080-O1 is coded as A1 and FB121-F1 is coded as D1 in the data processing and analysis.