Mice tumor scRNAseq
Gene expression of 16 fixed samples was evaluated following Chromium Fixed RNA Profiling (Gene Expression FLEX). Fixed samples were hybridized with barcoded probes and the sequencing library was generated following the User Guide (CG000527). Fixed cell sample concentration was evaluated with the automated cell counter LunaFX7, before hybridization step. Samples were hybridized with the 16-barcoded probe kit. Incubation of the samples at 42 degrees was performed for 22 hour and 30 min.