GSM8757685: WT107_S5; Mus musculus; RNA-Seq
Following euthanization of Slc4a11-/- and Slc4a11+/+ mice at 10 weeks of age (3 males and 3 females each), each globe was removed, and the Descemet membrane was separated from the posterior surface of each cornea.  The Descemet membranes from Slc4a11-/- mice were pooled, as were the Descemet membranes from Slc4a11+/+  mice were pooled and immediately processed for total RNA isolation with genomic DNA digestion using RNeasy Plus Micro Kit (Qiagen). Purified total RNA was then prepared for RNAseq libraries using SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (634411, Takara Bio USA). Libraries were sequenced on Novaseq S1, and paired-end 50-bp reads were generated.