GSM9461186: cultured cortical astrocyte, hM3Dq, 3days, 3; Mus musculus; RNA-Seq
Total RNA was collected from cultured astrocytes after 90 minutes after DCZ application, using Phenol-chloroform based RNA extraction. For, experiment aimed at assessing the long-term effect of DREADD stimulation, the cells were treated with DCZ for 90 min and the medium was replaced to a fresh medium without DCZ. Then, the RNA was collected from the culture three days after. RNA libralies for RNA-seq were prepared by Novogene. Briefly, the purified total RNA samples underwent mRNA purification, poly-A mRNA enrichment library preparation.