Raw reads: Mutation Accumulation line 89 (generation 32) ONT reads
Since the exact same DNA sample was used for both HiFi and ONT sequencing, HMW DNA extraction and DNA purification were performed as described for the corresponding HiFi libraries. ONT library preparation was performed using the Ligation Sequencing gDNA Native Barcoding Kit 24 V14 (SQK-NBD114.24, Oxford Nanopore Technologies). The resulting libraries were loaded onto FLO-PRO114M flow cells, and sequencing was conducted on a PromethION 2 Solo platform at Max Planck-Genome-Centre Cologne (MP-GC).