16S RNA of gut microbiome
Preparation of the DNA libraries was performed according to the Illumina protocol (Part #15044223, Rev. B.) with primers targeting the V3V4 regions of the SSU ribosomal RNA (rRNA) gene, S-D-Bact-0341-b-S-17 (5-CCTACGGGNGGCWGCAG-3) as the forward primer and S-D-Bact-0785-a-A-21 (5-ACTACHVGGGTATCTAATCC-3) as the reverse primer.