GSM8457615: WT HEK293T cell line - Replicate 1; Homo sapiens; RNA-Seq
Total RNA was isolated from 1 x 106 cells using RNeasy Plus Mini Kit (Qiagen) with genomic DNA removal. RNA was confirmed to be of high quality using Agilent Tapestation with RNA screentape (RIN >9.8 for these samples). 500ng was prepared for RNA-seq using Truseq RNA library preparation protocol with poly-A capture (Illumina). Libraries were sequenced on an Illumina NextSeq 2000 to produce 65bp paired-end reads.