RNAseq for A20
After RiboGreen quantification and quality control by Agilent BioAnalyzer, 1 µg of total RNA with DV200 percentages varying from 30-69% underwent ribosomal depletion and library preparation using the TruSeq Stranded Total RNA LT Kit (Illumina catalog # RS-122-1202) according to instructions provided by the manufacturer with 8 cycles of PCR. Samples were barcoded and run on a NovaSeq 6000 in a PE100 run, using the NovaSeq 6000 S4 Reagent Kit (200 cycles) (Illumina).