ONT reads of Drosophila virilis strain 140 genome
Females of strain 140 collected for DNA extraction were aged for 10-15 days, starved for 6h. Genomic DNA was isolated from thirty pairs of ovaries using high-salt denaturing buffer containing 100 mM Tris-HCl (pH = 8), 10 mM EDTA (pH = 8), 350 mM NaCl, 2% SDS and 7 M Urea following phenol-chloroform extraction. Size selection of high mo-lecular weight DNA was performed using Short Read Eliminator Kit (Circulomics, USA).   DNA libraries for sequencing were prepared using the 1D ligation SQK-LSK109 kit (Oxford Nanopore Technologies (ONT), UK) following the recommended protocol from ONT using 1 g of DNA. Sequencing was performed on a flow cell FLO-MIN-106D r9.4 (ONT, UK). Conversion of FAST5 to FASTQ format was performed with Guppy 6.4.6 using the super accurate flip-flop algorithm (dna_r9.4.1_450bps_sup.cfg). Quality filtering (Phred > 10) was completed by NanoFilt. The adapter sequences were removed with Porechop.