GSM8683234: day 7 Dox treatment 3; Homo sapiens; ATAC-seq
Organoids were collected on days 3, 5, and 7 following the initiation of Dox treatment, along with matched controls that were not treated with Dox. For each sample, 50,000 cells were lysed and fragmented using 2x TD Tagment DNA Buffer containing TDE1 Tagment DNA Enzyme (Illumina), Digitonin (Promega), and Protease Inhibitor Cocktail (Roche). After a 30-minute incubation at 37°C, samples were purified using the MinElute Kit (Qiagen). The libraries were sequenced on a NovaSeq 6000 instrument (Illumina) following a 50-base-pair, paired-end recipe.