GSM8993151: aPVAT Ctr M3; Mus musculus; RNA-Seq
tPVAT and aPVAT were collected, snap frozen in liquid nitrogen and stored at -80˚C. Frozen samples were pulverization in a liquid nitrogen pre-cooled metal cell crusher and total RNA was extracted by TRIzol (Invitrogen). Tissue powder was mixed with TRIZol and incubated under constant rotation for 15min at 4˚C for full lysis and centrifuged to pellet cell debris. Supernatant was used to purified total RNA using chloroform, Ethanol and RNeasy Micro Kit (Qiagen). At the Rockefeller Genomics Resource Center RNA quality was measured by the Agilent 2100 bioanalyzer chip and samples prepared with Illumina Stranded mRNA Prep, Ligation (96 Samples) /Catalog 20040534 with IDT for Illumina RNA UD Indexes Set B, Ligation (96 Indexes, 96samples).