GSM8546400: immune cells, DMBA/TPA induced tumor, sub library 5; Mus musculus; RNA-Seq
Tumors were collected 2 days after last dose of immunotherapy and total CD45 positive immune cells were sorted. Immediately after sorting, the sorted immune cells were fixed with Evercode Cell Fixation v2 (Parse Biosciences). the fixed cells were prepared using Evercode WT v2 kit (Parse Biosciences) according to manufacturer's instructions. Input cell numbers and volumes were calculated using WT_100K_Sample_Loading_Table_V1.3.0 (Parse Biosciences). All the samples were processed together with Evercode WT v2 kit (Parse Biosciences) and 8 sub-libraries were generated. The quality of sub-libraries was checked by the Agilent Tape Station system (Agilent) and sequenced by Illumina NovaSeqX.