RNA-Seq of homo sapiens: Peripheral  Blood Mononuclear Cells
Total RNA was quantified with a Thermo Fisher Scientific Qubit Fluorometer. Samples were assessed for quality using an Agilent 2100 Bioanalyzer and samples with an RNA integrity number (RIN) of 7 or greater were used as input to Takara's Clontech SMART-Seq v4 library amplification kit starting with 10pg-10ng RNA. Libraries were sequenced (Paired Read 2x150 basepairs) on an Illumina NextSeq 500 sequencer.