GSM9677648: Pseudomonas syringae pv. porri CFBP 1770, infected with phage KIL3b; control; Pseudomonas coronafaciens pv. porri; Pseudomonas phage vB_PsyM_KIL3b; OTHER
All samples were pooled together and homogenized. The resulting sample was subjected to hot phenol treatment and ethanol precipitation to extract and purify the RNA Total RNA was enriched for primary transcripts using the ONT-cappable-seq enrichment protocol. In parallel, control samples were subjected to similar environmental conditions. The RNA was reverse transcribed, PCR amplified and barcoded accoriding to Oxford Nanopore Technology cDNA-PCR protocol (SQK-PCB109)