16S rRNA amplicons of microbial community in Day0QD (CRX2128971)
The 16S rRNA gene V4 region was amplified using primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3'). PCR products were purified and used to construct paired-end sequencing libraries following the Illumina standard protocol. Sequencing was performed on an Illumina HiSeq 2000 platform with 2 x 100 bp read length.