GSM9462225: A2780 cells, lycorine treatment-2; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol reagent (15596018CN, Thermo Fisher Scientiffc, MA, USA). Using Oligo dT magnetic beads, mRNA is enriched from total RNA. After fragmentation, the first strand of cDNA is synthesized using random hexamer primers, followed by the synthesis of the second strand of cDNA. After end repair, A-tailing, adapter ligation, fragment selection, amplification, and purification, the library is ready.