GSM9737686: mHSC sc-lentiMPRA Library Alpha, GFP quantitative barcode, sublibrary S6; Mus musculus; RNA-Seq
After a ~20 day expansion period, HSPC cultures were infected with the lentivirus-packaged libraries (Library PoC, Library Alpha, Library Beta).  7 days following initial infection, live GFP+ cells harbouring lentiviral integrations were collected by FACS with a permissive GFP gate. Another batch of cells was collected with the same FACS strategy after another 7 days in differentiation media. Library construction was performed following the manufacturers protocol using the 10x Genomics Next GEM Single Cell 5'Kit with adjustments and custom PCRs that we describe in the methods of the manuscript.