Analysis of differential circRNA expression in mouse retina isolated from mice that harbor the Y2334N mutation in both alleles of the pre-mRNA splicing factor PRPF8
Analysis of differential circRNA expression in mouse retina isolated from mice that harbor the Y2334N mutation in both alleles of the pre-mRNA splicing factor PRPF8
The mice have GFP-tagged PRPF8 on both alleles with the Y2334N mutation for the mutant samples, and no mutation for the control samples. The neural retina was isolated from the mice and immediately frozen in dry ice. Neural retina samples were resuspended in 300 µL of TRIzol and then stored at -80°C. The total RNA was isolated from each sample using the Direct-zol RNA MicroPrep Kit (Cat. No R2062) according to the manufactuerer's instructions. Then, each sample was on-column DNase I treated. Finally, RNA was eluted in 11 µL of DNase/RNase-free water. Ribodepletion and library preparation was done with KAPA RNA HyperPrep Kit with RiboErase KK8560. Then the quality and length distribution was mesured using Agilent 2100 Bioanalyser and Qubit. Pooling was done and primer-dimers were cleaned using Spri-selected beads. A final pool quality and length distribution was measured with Agilent 2100 Bioanalyser and Qubit.