GSM9735742: 526MEL treated with supernatant replicate 1; Homo sapiens; RNA-Seq
Total RNA from two biological samples was individually isolated using the RNeasy Mini kit and treated with DNase I (Life Technologies, Gaithersburg, MD, USA), according to the manufacturer's protocol. In addition, 0.5 μL of RNase inhibitor (Nippon Gene, Toyama, Japan) was added to each tube before the RNA purification. RNA sequencing was performed by Novogene, where directional mRNA libraries were prepared using poly(A) enrichment and dUTP-based strand-specific library construction.