16S amplicon sequencing of samples resulting from mock dissections with synthetic community standard spike-in: blank 2+spike-in
DNA was extracted from a sample obtained by performing a mock mosquito tissue dissection and collection and used as a template in PCR with 515F and 805R primers with Illumina adapters. PCR product was isolated on an agarose gel and 0.5 ng of synthetic microbial DNA (ZymoBIOMICS Microbial Community DNA Standard) was spiked in. The sample was subjected to 300 base pair paired-end sequencing on an Illumina NextSeq 2000 platform following the Illumina 16S Metagenomic Sequencing Library Preparation guide.