GSM8859042: RNAseq_Donor1_AAVS1; Homo sapiens; RNA-Seq
Total RNA was purified using an RNeasy Mini Kit (Qiagen, #74104). Genomic DNA was isolated after tagmentation using Qiagen MinElute Reaction Cleanup Kit (Qiagen #28204). Ribosomal RNA was removed using the NEBNext rRNA Depletion Kit (New England Biolabs, E6310L). RNA was then prepared with the Illumina Stranded Total kit (Illumina, 20040525).