GSM9305687: 2D_WT_shGFP_E2_R50_001; Homo sapiens; RNA-Seq
RNA extraction was performed using RNeasy Mini Kit (Qiagen). Purity of the total RNA samples was assessed using BioAnalyzer (Agilent) and samples with an RNA integrity score >8 were used for library construction by the University of Minnesota Genomics Core. Libraries were built from individual samples with the TruSeq Stranded mRNA kit (Illumina).