RNA-Seq of Homo sapiens: adult female endometrium
For mRNA library preparation, total RNA was processed using the Illumina Stranded mRNA Prep Kit (20020595, Illumina, San Diego, CA, USA). mRNA was enriched using oligo(dT) beads targeting poly(A) tails, fragmented, and reverse transcribed using random hexamers to generate first-strand cDNA. Strand specificity was achieved by incorporating deoxyuridine triphosphate (dUTP) during second-strand synthesis. The resulting double-stranded cDNA was ligated to indexed adapters and amplified by PCR.