GSM8992884: Mouse_DMSO_5_2; Mus musculus; RNA-Seq
Single-cell cDNA synthesis was performed using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara) according to the manufacturer's instrictions. Briefly, single-cells were manually picked after dissociation and snap frozen on dry ice in reaction buffer in low-bind 0.2 ml PCR tubes and stored at -80°C until processing. Samples were subject to first strand cDNA synthesis, then cDNA was amplified by LD-PCR for 23 cycles. Amplified cDNA was purified on AMPure XP beads, using a 96-well magnetic stand (ThermoFisher), and eluted in 17 µl of elution buffer. The libraries were prepared using the Nextera XT DNA Library Preparation Kit (Illumina) according to the manufacturer's protocol. Sequencing was carried out as paired-end 50bp read on a Novaseq 6000 sequencer.