GSM8718784: RPE, Async, EU-RNA-seq; Homo sapiens; OTHER
EU-RNA-seq was performed according to a commercially available kit (Click-iT™ Nascent RNA Capture Kit, Invitrogen). Briefly, about 2,000,000 cells were labelled with EU, and total RNA were extracted, followed by biotinylation by click reaction. Biotinylated RNA was then precipitated with ammonium acetate, and captured using Streptavidin T1 magnetic beads, followed by cDNA systhesis. Sequencing of the library was conducted on Illumina Novaseq (PE150) platform (Novogene).