Transcriptome: Pure culture
First, the total RNA of the sample was extracted, the ribosomal RNA was removed, fragmentation buffer was added, and the random interruption was 213 nt fragments. The first strand of cDNA was synthesized by adding six-base random primers, buffers, dNTPs, RNase H and DNA polymerase I, dUTP was used instead of dTTP, the second strand of cDNA was synthesized, the end was repaired, base A was added, sequencing adapters were added, and the second strand of cDNA was degraded using UNG enzyme to preserve RNA orientation information. PCR amplification, library preparation was completed, and the constructed library was sequenced using the Illumina platform