GSM9738131: VCaP.8hr.PBITE.1.50uM.2; Homo sapiens; RNA-Seq
Total RNA was isolated using Trizol and an RNeasy Kit (Invitrogen) with DNase I digestion according to the manufacturer's instructions. Ribosomal RNA was depleted using the RiboErase module of the KAPA RNA Hyper+RiboErase HMR Kit (Roche, cat. no. 08098140702), and the ribosomal RNA-depleted RNA was used for library preparation following the manufacturer's protocol. Library quality and concentration were assessed using the Agilent 2100 Bioanalyzer, and libraries were sequenced on an Illumina NovaSeq 6000 using paired-end 150 bp reads at a depth of 15–20 million paired reads per sample.