GSM9533761: H2A-mCherry cells, Rapamycin and PDGF treatment, replicate 3; Mus musculus; RNA-Seq
Total RNA was extracted using RNA extraction kit (Zymol).1 µg of total RNA was used for the construction of sequencing libraries. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.