GSM9753031: Blood monocytes, 6 hours post dose 2, mouse ID MOD845; Mus musculus; RNA-Seq
RNA was extracted using RNeasy midi kit (Qiagen) according to the manufacturer's instructions. RNA concentration and quality were assessed using a 2100 Bioanalyzer (Agilent). RNA libraries for RNA-seq were prepared using the SMARTer Ultra Low + Nextera XT kit from Illumina according to the manufacturer's instructions. 100 ng of total RNA were used for the construction of RNA-seq libraries. Library molarity and quality were assessed with a Qubit and Tapestation using a DNA high sensitivity chip (Agilent Technologies). Libraries were pooled at a 1.1 nM concentration and sequenced with an Illumina NovaSeq 6000 sequencer for a minimum of 30 million single-end 100 bp reads per sample.