16S rRNA gene sequencing of Mule duck: cecal microbiome from CF-F group
16S rRNA gene V3-V4 region amplicon sequencing was performed to analyze gut microbiota composition in Mule ducks under different feeding regimens. Three experimental groups were included: Control (CON) with free access to feed (~300 g/day dry matter), Controlled force-feeding (CF-F) with equivalent intake, and Conventional force-feeding (F-F) with thrice-daily feeding (500 g to 1 kg/day). DNA was extracted from jejunal and cecal samples collected after an 36-day experiment, followed by PCR amplification and sequencing on an Illumina MiSeq platform.