GSM9257894: B003; Homo sapiens; RNA-Seq
Total RNA was extracted from PMN samples using the RNeasy Plus Universal Mini Kit (Qiagen, Germantown, MD) according to the manufacturer's protocol. ). Sequencing libraries were prepared using the Illumina RNA-Seq Library Prep Set for low-input RNA, following the manufacturer's guidelines. Libraries were quantified using qPCR and assessed for fragment size distribution using the Agilent Bioanalyzer before pooling.