GSM9258139: ChIPseq_Flower_b2_CLSY3-3xFLAG_clsy3_delta3-396_IP; Arabidopsis thaliana; ChIP-Seq
Genomic DNA were extracted from flower buds or shoot tissues using CTAB method or using the DNeasy Plant Mini Kit (QIAGEN, Cat# 69014). 1 g of flower buds (stage12 and younger) were collected and ground in liquid nitrogen. ChIP experiment using the anti FLAG antibody (Sigma, Cat# M8823) was perfomed following the protocol described in the Materials and methods. The DNA was isolated using Phenol/Chloroform/IAA (Sigma, Cat# 6805), precipitated by ethanol, and resuspended in 20 μL of H2O. Library preparation was conducted using the NEBNext Ultra II DNA Library prep kit (NEB, Cat# E7645L) for sequencing.