Posttranscriptional reprogramming controls MASLD progression through chronic ER stress adaptation (RIP Seq)
Posttranscriptional reprogramming controls MASLD progression through chronic ER stress adaptation (RIP Seq)
Animals were euthanized and livers were collected. Primary hepatocytes were isolated after tissue dissociation. Male C57BL/6J mice were housed under standard conditions. At 6 weeks of age, mice were randomly assigned to either a high-fat diet (HFD) or a normal diet (ND) and fed HFD or ND for 30 weeks when the experiments were perfomed. RNA was isolated using TRI-sure reagent (Bioline) according to the manual, using Glycoblue (Ambion) as a carrier. RNA libraries were prepared for sequencing using the kit NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (Cat#E7770), following manufacturer´s instructions and 10-11 cycles of amplification.