Single nuclei RNA-sequencing of placental organoids generated from CT27 and CT29 cell lines
Single nuclei RNA-sequencing of placental organoids generated from CT27 and CT29 cell lines
CT27 and CT29 cell lines were cultured and eventually they were flash-frozen Both the CT27 and CT 29 lines were maintained on 5 Âµg/mL collagen IV coated plates and using human TSC culture medium in a humidified incubator at 37 degrees and 5% CO2 nuclei for both cell lines were collected using the 10X Chromium Nuclei Isolation Kit with RNAse inhibitor following the manufacturer's instructions.  Libraries were prepared using the 10X GEM-X Universal 3' Gene expression v4 4-plex according to manufacturer's instructions.