Whole Genome Sequencing of Pseudomonas aeruginosa
gDNA was repaired using end-prep mix to generate 5 phosphorylated, 3 dA-tailed ends, followed by adapter ligation (ONT SQK-NBD114-24). Libraries were quantified with Qubit and loaded onto FLO-MIN114 flow cell. Sequencing was performed on GridION using MinKNOW (v23.07.12), and basecalling was done with Guppy (v7.1.4, HAC mode). Run config: Bream v7.7.6, Config v5.7.11, MinKNOW Core v5.7.5.