16S of Podocnemis unifilis: unhatched egg
We extracted bacterial genomic DNA from swab samples following standard protocols for the Nucleo Spin 96 Soil extraction kit (Macherey-Nagel,Germany). We performed a two-step PCR with the primers 515 Fand 806R to sequence a 291-bp fragmen to the hypervariable V4 region of bacterial 16S rRNA and the primers ITS1F and ITS4R to amplify the ITS region. Finally, we followed the Fluidigm (Acces Array Barcode Library for Illumina Sequencers-384, Single Direction, Fluidigm Corporation, South San Francisco, CA, USA) protocol for sequencing on the Illumina MiSeq sequencing platform