GSM8487148: epithelial cells, Mal04-Cre infected mice, 3 dpi, infected; Mus musculus; RNA-Seq
Mice were anesthetized with ketamine-xylazine followed by cervical dislocation and lung removal. The lungs were perfused with PBS then inflated with digestion enzymes. Lungs were removed minxed finely with a razor blade then incubated in digestion enzyme 30 minutes. The tissue mixture is filtered through a 70-µm filter and red blood cells were lysed with RBC lysis buffer and the remaining cells were filtered through a 40-µm filter. Spin the cells down and resuspend in 2% FBS with advanced DMEM/F-12 to be sorted on a BD DiVA. Sorted cells were prepared for library generation (10x Genomics) according to manufacturers protocols.