GSM9533367: HT-29 CTL TNF+IFN, biol rep 1; Homo sapiens; RNA-Seq
Cells were harvested and total RNA was extracted using the All Prep DNA/RNA/miRNA Universal Kit (#80224, Qiagen, MD, USA). polyA-enriched stranded RNA libraries were prepared. 100 bp paired-end sequencing (PE100, 25M reads) was performed on an Illumina NovaSeq 6000 at the McGill University and Genome Quebec Innovation Centre (Montreal, QC, Canada).