GSM8682987: ELOA-dTAG_dTAG13_HA-ELOA; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 15 minutes. After quenching and cell lysis,  the nuclei were transferred to 1ml milliTUBEs with AFA fibers (Covaris, 520130) and sonicated in a Covaris E220 focused ultrasonicator (duty factor: 10%, peak intensity pulse: 140, cycles per burst: 200, time: 500 seconds). Libraries were made using the KAPA HTP Biosystems Library Prep kit (Roche, KK8234) using Perkin Elmer Unique Dual Index Barcodes for Illumina NextSeq 2000