Ilumina RNA-Seq of Chrysina beyeri, replicate 1
Total RNA was extracted from thoracic tissue of an adult Chrysina beyeri (biological replicate 1) collected from USA: Arizona, Cochise County, on 2023-08, using the Qiagen RNeasy Mini Kit. Poly-A-selected mRNA was isolated using oligo(dT) beads, and a stranded RNA-Seq library was prepared with the Illumina TruSeq Stranded mRNA Library Prep Kit. The library was pooled with other samples and sequenced on an Illumina NovaSeq 6000 (S4 flowcell HGH3LDSX7, lane 3) at the Texas A&M AgriLife Genomics and Bioinformatics Service on 2023-08-03, yielding 2  151 bp paired-end reads. These reads were used as transcript evidence for BRAKER3 gene annotation of the Chrysina beyeri genome assembly.