NC_group_NC1
The V3V4 region was amplified using standard primers (F 5-CCTAYGGGRBGCASCAG-3 and R 5-GGACTACNNGGGTATCTAAT-3), and purified PCR products were quantified with Qubit and used to construct libraries with the NEB Next Ultra DNA Library Prep Kit. Sequencing was performed on an Illumina platform to generate 250 bp paired-end reads.