YUSEEP, MeWo, M160113, WM4235, Sk-Mel-24, M141207, YUHIMO, co-processed cultured human cancer cell lines used for EasySci single-cell RNA sequencing
Nuclei isolated from cultured human cancer cell lines were processed using the EasySci-RNA protocol. Reverse transcription, ligation-based barcoding, and indexed PCR amplification generated single-cell transcriptome libraries, sequenced on an Illumina NextSeq 1000 (paired-end 100 bp; dual 10 bp index reads).