H.d. cell aggregates (24h after dissociation) (Mar21), replicate 1
Extracted RNA was treated by Dnase I (Ambion) according to the DNA-free Kit (Ambion) protocol. For 50 bp single-end sequencing mRNA was isolated using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) from 300 ng of total RNA and cDNA library of each sample was constructed using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instruction. The cDNA library quality and fragment length distributions were verified using an Agilent 2100 DNA High Sensitivity Kit.