GSM9821577: Sacubitrilat-treated hiPSC-CMs, biological replicate 2; Homo sapiens; RNA-Seq
Total RNA was extracted by Plasmidsaurus from cells preserved in Zymo DNA/RNA Shield Poly(A) mRNA was captured from total RNA using a poly(dT)VN primer containing a sample barcode, a unique molecular identifier (UMI), and the Read 1 sequence. Reverse transcription and second-strand cDNA synthesis were followed by tagmentation. Libraries were indexed and amplified to add unique dual indexes and Illumina P5/P7 adapter sequences. Libraries were prepared as stranded, single-end, approximately 90-bp 3-prime-end-counting RNA-sequencing libraries.