RNA-seq of peach without P. expansum: replication 3
A sterilized puncher was used to create uniform wounds at three different points with an area of 5  5 mm in the equatorial region of each peach fruit. The wounds were allowed to air dry at room temperature. Subsequently, 30 L of the P. expansum conidial suspension (1108 spores/mL) was applied to each wound. The treated fruit samples were placed in a plastic tray, covered with plastic film (to maintain high relative humidity), and stored in the dark at 25 C.