RNA-Seq of mus musculus: primary astrocytes
Sequencing libraries were generated using the Hieff NGSUltima Dual-mode mRNA Library Prep Kit(Cat#12309, China). A total amount of 0.2 g RNA per sample was used as input material for the RNA sample preparations. The first step is to enrich mRNA by Poly(A) method with mRNA Capture Beads(BOX-I 12603-A), after mRNA Purification and Fragmentation, the RNA fragments is concentrated and the homogeneity. followed by Synthesis of the 1st strand cDNA and the 2nd strand cDNA, 3' ends adenylated and 5' ends repaired, dA-tailing, Adapter Ligation, Library Amplication, At last, PCR products were purified (Hieff NGS DNA Selection Beads (Yeasen Cat#12601) and library quality was assessed on Qubit dsDNA HS FluorometerThermo,USA) and the Bioptic Qsep101 system.