Nanopore metagenomic sequencing of microbial community: enrichment plastic-associated fraction on low-density polyethylene (VT_7)
Metagenomic DNA was extracted from enrichment culture (plastic fraction) of Setun River microbial community incubated in M9 minimal medium with LDPE plastic fragments. Libraries were prepared using Oxford Nanopore ligation protocol with R10.4 chemistry and sequenced on Nanopore flow cells with subsequent basecalling and quality control.