GSM9164513: CITEseq, HTO; Homo sapiens; OTHER
Cultured cells were collected stained with TotalSeq anti-human hashtags C0251- C0258 (Biolegend), viability dye (zombie red, Biolegend 423109) and anti-human CD45-FITC (clone HI30, Biolegend 304038) and enriched for live cells and up to 50% immune cells using BD FACS Aria II. Sorted cells were then resuspended to 700-1200 cells per ul and barcoded for multiplexed single cell sequencing using 10x Genomics 5'v2 chemistry (10x Genomics, PN-1000263). Sorted cells were immediately loaded into a 10x Chromium reaction. Chromium Next GEM Single-Cell 5′ kits v.2 were used to generate GEX, VDJ and Cite-seq libraries according to the manufacturer's protocol.