GSM9207090: LSS3-input; Homo sapiens; RIP-Seq
Total RNA was extracted using TRIzol reagent (Invitrogen) according to the manufacturer's instructions. RNA integrity was assessed by Agilent 2100 Bioanalyzer. For acRIP-seq, 300 µg of total RNA was fragmented into ~100 nt fragments using RNA fragmentation buffer. Fragmented RNA was incubated with anti-AC antibody (e.g., Synaptic Systems, 202003) and immunoprecipitated with Protein A/G magnetic beads. The AC-enriched RNA and corresponding input RNA were used to construct sequencing libraries using NEBNext® Ultra™ RNA Library Prep Kit (NEB) following the manufacturer's protocol.