GSM8683199: SKBR3, Herceptin treated mRNA sample, replicate 8; Homo sapiens; RNA-Seq
Total RNA was extracted from the samples using the commercial Quick-RNA™ MiniPrep kit (Zymo Research, Irvine, USA) adhering to the protocol provided by the manufacturer. Before shipping the RNA concentration was quantified with the Qubit fluorometer, ensuring a minimum concentration threshold of 100 ng/μl prior to further processing. The cDNA library was prepared using TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, USA) and sequenced at the Next Generation Sequencing Facility of The Hospital for Sick Children in Toronto, Canada. A HiSeq 2500 platform (Illumina, San Diego, USA) was used to generate 2 x 126 bp paired end reads.