GSM9376730: O2_Spleen; Nothobranchius furzeri; RNA-Seq
RNA was harvested using ReliaPrep RNA Tissue Miniprep System (Promega GmbH). >10ng/ul of total RNA was used for the construction of sequencing libraries. RNA sequencing analysis was performed at Novogene GmbH (Munich, Germany). Total RNA quality and integrity were verified using the Agilent 5400 Fragment Analyzer (Agilent Technologies). Libraries were prepared following standard Illumina protocols, quantified with the Qubit™ dsDNA HS Assay Kit (Invitrogen), and validated for fragment size and integrity on the Fragment Analyzer. Final quantification was performed by qPCR. Sequencing was conducted on an Illumina NovaSeq X Plus platform (paired-end 2 × 150 bp).