GSM9853367: RSL3+M-1.R1; Homo sapiens; RNA-Seq
Total RNA was extracted from cells using TRIzol® reagent (Invitrogen, USA) according to the manufacturer's instructions. RNA purity and integrity were assessed using a NanoDrop™ spectrophotometer and an Agilent 2100 Bioanalyzer. RNA‑seq libraries were constructed using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA, USA) following the manufacturer's protocol