GSM8683885: CHGA-Venus, ileum, scRNA-seq; Homo sapiens; RNA-Seq
Human duodenum and ileum organoids were generated from fully anonymised specimens provided by Addenbrooke's Hospital Tissue Bank. Human small intestinal organoids were cultured in a differentiation medium for 5–10 days before enzymatic and mechanical dissociation to single cells. Single cells were sorted for Venus fluorescence using an Influx Cell Sorter (BD Biosciences) at the Cambridge Institute of Medical Research (CIMR) Flow Cytometry Core Facility. DAPI and DRAQ5 staining, side scatter, forward scatter and pulse width gates were used to remove debris and cell clusters. cDNA library preparations were performed using the Chromium system (10X Genomics) by the Genomics and Transcriptomic Core at the Institute of Metabolic Science.