GSM8940329: HEepG2-shNT-1-Input; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. ChIP-seq was performed as previously described using MNase digestion, with the Drosophila genome included as a spike-in control for normalization. For each sample, 30 µg of chromatin was incubated overnight at 4°C with antibodies against Kpm, Kac, or Kpr. ChIP-seq libraries were constructed according to the manufacturer's instructions using the SimpleChIP® Plus Enzymatic Chromatin IP Kit (Cell Signaling Technology). The libraries were sequenced on an Illumina HiSeq 2500 platform using 50 bp single-end reads.