GSM8682797: CD8 T cells NK depleted; Mus musculus; RNA-Seq
Nine days after tumor implantation, MC38 tumor samples were collected from both control and NK cell-depleted mice. The harvested tumor samples were mechanically minced and enzymatically digested using collagenase types I and IV. The digested samples were neutralized with BSA and filtered through a 70 μm cell strainer. The resulting single-cell suspension was stained with a viability dye, followed by surface marker staining. Live CD8+ T cells were then sorted using the BD FACS Melody system. Library construction was performed according to the manufacturer's instructions (single cell 5' V2 protocol, 10X genomics).