16S rRNA sequencing of sludge pretreated with 45 mmol/L PAA
Total genomic DNA was extracted from dewatered sludge samples using the FastDNA Spin Kit for Soil (MP Biomedicals, USA) according to the manufacturers instructions. The V3V4 hypervariable regions of the bacterial 16S rRNA gene were amplified using primers 338F (5-ACTCCTACGGGAGGCAGCA-3) and 806R (5-GGACTACHVGGGTWTCTAAT-3). PCR products were purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, USA), quantified, and pooled in equimolar concentrations. Sequencing libraries were prepared using Illumina TruSeq DNA PCR-Free Library Preparation Kit