CAGE of aposymbiotic H. viridissima clone 2
CAGE (Cap Analysis of Gene Expression). Custom CAGE protocol: dephosphorylation, 3' blocking, Decapping enzyme (NEB), 5'-Biotin-ApG ligation (Jena Bioscience), RT, RNase If, streptavidin capture, alkali release. Library: Zymo-Seq RiboFree kit (Sections 3-4).