GSM8486840: SCO Hg Fed 3; Fundulus heteroclitus; RNA-Seq
Total RNA was isolated from 6 embryos' brains per treatment from individual 17 dpf embryos using the Qiagen RNeasy® Micro Kit (Germantown, MD, USA) following the Purification of Total RNA from Animal and Human Tissues protocol in the RNeasy® Micro Handbook with slight modifications.  The modification included homogenization of brain tissue in 350 µL of RLT buffer using a pellet pestle and elution of Total RNA using 15 µL of RNase-free water. RNA quality was assayed using the Agilent High Sensitivity RNA ScreenTape System (Waldbronn, Germany) for the Agilent 2200 TapeStation (Palo Alto, CA, USA), and RNA was quantified using the NanoDrop 2000 (ThermoFisher Scientific, Waltham, MA).