GSM9820635: Stingfl/fl Cdh5-Cre+ mouse liver scRNA-seq, biological replicate 2; Mus musculus; RNA-Seq
Liver tissues were dissociated into single-cell suspensions. Cells were washed, filtered, and resuspended in PBS with 0.04% BSA for 10X Genomics library construction. Single-cell RNA-seq libraries were prepared using the 10X Genomics Chromium 3' reagent kit according to the manufacturer's protocol. Briefly, single cells were partitioned into gel beads in emulsion (GEMs) for reverse transcription. Barcoded cDNA was amplified, fragmented, end-repaired, A-tailed, and ligated with adapters for sequencing.