Pri-miRNA Expression Profiles in Burkitt and Hodgkin lymphoma cell lines upon Ionizing Radiation Exposure
Pri-miRNA Expression Profiles in Burkitt and Hodgkin lymphoma cell lines upon Ionizing Radiation Exposure
Burkitt lymphoma and Hodgkin lymphoma cell lines were exposed to ionizing radiation at a dose of 4 Gy and collected 4h and 12h upon irradiation, control unirradiated cells were collected at 4h timepoint.  Total RNA was extracted using the miRNeasy Mini Kit  (QIAGEN, Cat. No. 1038703). This kit is specifically designed for the isolation of high-quality RNA, including small RNAs such as miRNA, from a variety of sample types. After addition of chloroform, Heavy 5PRIME Phase Lock GelTM (QuantaBio, Cat. No. 2302830) was applied to improve organic/inorganic phase separation. Upon isolation, RNA was treated with and RNase-Free DNase Set (Qiagen, Cat. No.  79254), to remove possibly remaining DNA.  Libraries were generated with Illumina TruSeq Stranded Total RNA with Ribo-Zero H/M/R_Gold. Standard fragmentation conditions were used (90°C for 2 minutes to obtain 150 nt sequences (modified from standard 94°C for 4 minutes).