Oncomine Tumor Mutation Load (TML) assay of lung cancer patient
20 ng DNA from FFPE tissue was treated with Uracil-DNA Glycosylase (UDG). Library preparation of the Oncomine Tumor Mutation Load (TML) Assay was done with the Ion AmpliSeqTM Library kit 2.0. Template preparation and enrichment were performed with the Ion Chef system. Six barcoded samples were pooled per Ion 550 TM chip and sequenced on the Ion GeneStudio S5TM System.