GSM8506702: RNA-seq_ZT4_L; Glycine max; RNA-Seq
Approximately 0.5 g of soybean mature leaves were used in each experiment. About 1 to 2g of the sample was ground into a fine powder in liquid nitrogen. Total RNA was isolated from mature rice leaves using the RNeasy Plant Mini Kit according to the manufacturer's recommendations (QIAGEN, 74904). Ribosome RNA was removed from 0.5 to 1.5 g of Total RNA using a kit TruSeq Stranded Total RNA with Ribo-Zero Plant for RNA-seq according to the manufacturer's recommendations.