Manipuri horse breed mitogenome
The venous blood samples collected aseptically through jugular vein puncture by vacutainer containing Ethylene Diamine Tetra Acetic Acid (EDTA) as an anticoagulant were stored at 4C for further analysis. Genomic DNA was isolated from the blood samples through spin column method as per standard protocol (Sambrook and Russel, 2001). The integrity and the quality of extracted DNA were checked by electrophoresis on 1 % agarose gel and visualised under Gel-documentation System. The quality and concentration of DNA were checked by utilising the Nano Drop Spectrophotometer. The DNA samples having concentration greater than 50ng/l and the OD ratio (A260/A280) between 1.6-1.8 were used further for analysis.After quality check, the genomic material was subjected to fragmentation, adapter ligation and amplification. The sequencing was performed on the Novaseq 6000 platform employing paired-end methodology (2  150 bp reads) with a target coverage depth of at least 10X.