RNA sequencing of non-stimulated (non-stim) or lipopolysaccharide (LPS)-stimulated (100 ng/mL, 1 h) wildtype (WT) and mt-tRNAala m.5019A>G mutant murine bone marrow-derived macrophages (BMDMs). Three biological replicates per condition
RNA sequencing of non-stimulated (non-stim) or lipopolysaccharide (LPS)-stimulated (100 ng/mL, 1 h) wildtype (WT) and mt-tRNAala m.5019A>G mutant murine bone marrow-derived macrophages (BMDMs). Three biological replicates per condition
BMDMs were plated at 0.5 x 106 cells/mL (2 mL total volume) in a 6-well dish and left to adhere overnight at 37oC in a de-humidified incubator (21% O2, 5% CO2) before being treated with LPS (100 ng/mL) or left untreated. RNA isolation was carried using RNeasy® Plus kit (74136, Qiagen) following manufacturer's suggestions and eluted RNA was purified using RNA Clean & Concentrator Kits (Zymo Research). RNA-seq samples libraries were prepared by Cambridge Genomic Services (CGS) using TruSeq Stranded mRNA (Illumina).