wildtype 8w (CRX1236290)
We used GEXSCOPETM Single Cell RNA Library Kit Tissue (Singleron Biotechnologies) to barcode single cells, capture mRNA from isolated single cells, and generate cDNA libraries for scRNA-seq. Then, individual libraries were diluted to 4 ng/ul, and pooled for sequencing on a HiSeq X platform (Illumina, San Diego, USA) with 150 bp paired-end reads.