Whole‑genome sequencing using the Illumina DNA Prep workflow involves tagmentation, where bead‑linked transposomes simultaneously fragment genomic DNA and add adapter sequences. The tagged DNA is then cleaned and amplified by limited‑cycle PCR to incorporate full sequencing adapters and indexes. Libraries are purified using bead‑based size selection, quantified, optionally pooled, and diluted to the appropriate concentration before sequencing on an Illumina platform. This streamlined workflow supports a wide DNA input range, produces normalized library yields, and enables efficient preparation for high‑throughput whole‑genome sequencing.