Transcriptome of primary cystic fibrosis human bronchial epithelial cells incubated with SLC6A14 inhibitor (alpha-methyltryptophan)
Transcriptome of primary cystic fibrosis human bronchial epithelial cells incubated with SLC6A14 inhibitor (alpha-methyltryptophan)
RNA extraction was from cells that were incubated with MetOH or a-MT for 3, 6 or 24 hours.  RNA were extracted using a NucleoSpin RNA/miRNA kit (Macherey-Nagel, Duren, Germany) The libraries were prepared following the TruSeq Stranded mRNA protocol from Illumina, starting from 400 ng of good quality total RNA (RIN >7). 