Single-cell RNA-seq of hemocytes in adult female sugar-fed Anopheles gambiae injected with nuclease free water
The aim is to investigate the transcriptional changes in the different hemocytes of adult Anopheles gambiae in response to activation and suppression of immune pathways  leading to altered hemocyte differentiation and function. Single-cell RNA sequencing was performed on fixed hemocytes collected from mosquitoes injected with control (dsLacZ injected, uninjected and nuclease free water injected) and dsRNA against immune pathway regulator genes (Cactus, Rel1,Jnk, Puckered, SOCS,STAT-A,Rel2 and Caspar) using the 10X Chromium 3 kit and Illumina paired-end sequencing.