GSM8994513: SK-N-BE, siTRMT6，m1A-1; Homo sapiens; RIP-Seq
transfection siRNA target TRMT6 to redece its expression m1A-map-seq was performed as following. In brief, Dynabeads Oligo (dT) purified mRNA was fragmented using NEBNext® Magnesium RNA Fragmentation Module (NEB, USA) and immunoprecipitated using m1A antibody (MLB). The immunoprecipitated m1A RNA fragments were extracted with Trizol reagent (Thermo Fisher, USA). Then, input sample without immunoprecipitation and m1A IP RNA fragments respectively generated libraries.