GSM9136711: OncoD; Mus musculus; RNA-Seq
Tumor-bearing brain hemispheres of OncoD- or OncoD-0J1-treated mice were dissociated into single-cell suspension using the Tumor Dissociation Kit and gentleMACS Octo Dissociator from Miltenyi Biotec. Mouse anti-F4/80 MicroBeads UltraPure from Miltenyi Biotec were used to isolate F4/80+ cells which were submitted to single-cell RNA sequencing. Cells were loaded onto the Chromium X instrument (10X Genomics) and processed using the Chromium Next GEM Single Cell 3ʹ Reagent Kit v3.1 Dual Index (10X Genomics, cat# 1000268) according to the manufacturer's protocol. scRNA-seq libraries were generated following standard procedures. Quality control (QC) was performed before sequencing using the Agilent 2100 Bioanalyzer and 2200 TapeStation (Agilent, Santa Clara, CA, USA). Library concentration was measured with a Qubit Fluorometer (Thermo Fisher). Sequencing was carried out on the NovaSeq 6000 system (Illumina Inc., San Diego, CA) following 10X Genomics guidelines.