metatranscriptomics3 (CRX2121748)
The library construction workflow was performed separately for DNA and RNA. DNA libraries were prepared using the Nextera DNA Flex Kit, while RNA sequencing libraries were constructed using the Ovation Trio RNA-Seq Library Preparation Kit. Following preparation, all libraries were quantified using a Qubit dsDNA HS Assay Kit, and their integrity and quality were assessed with an Agilent 2100 Bioanalyzer and a High Sensitivity DNA kit. Finally, the qualified libraries were sequenced on an Illumina NextSeq 550 platform employing a 75-cycle single-end strategy.