GSM9531992: Colonic Organoids, Butyrate, Day 2; Mus musculus; RNA-Seq
Organoids were isolated from Matrigel domes by manual dissociation and incubation with TrypLE Dissociation reagent. Cells were lysed using the QIAzol Lysis reagent (Qiagen). Poly-A pulldown was employed to enrich mRNAs from total RNA samples prior to library construction using Illumina TruSeq chemistry. The final PCR step has been modified using the KAPA HiFi HotStart Ready Mix.