shotgun metagenomic library of homosapien: blank control
Genomic DNA was extracted from fecal material using a Maxwell RSC Fecal Microbiome DNA kit implemented on a Maxwell RSC device (Promega). Shotgun metagenome libraries were prepared using an Illumina DNA Prep kit (formerly Nextera FLEX). Libraries were evaluated initially using an Illumina iSeq instrument (2  150 base sequencing) followed by re-pooling and deep sequencing on an Illumina NovaSeq6000 SP flow cell (2  150 sequencing).