First report of stx2k-producing E.coli strains from food in Italy
Genomic DNA was isolated from each isolate using a columnbased extraction kit according to the manufacturers instructions. DNA concentration and integrity were evaluated using Qubit and Nanodrop measurements. Sequencing libraries were constructed using the Illumina DNA Prep kit, which performs enzymatic fragmentation followed by endrepair, adapter ligation with dual indexes, and beadbased purification. Libraries were quantified, normalized, and pooled prior to sequencing. Pairedend sequencing (2150 bp) was performed on an Illumina platform, generating raw FASTQ files. No additional preprocessing, trimming, or filtering was applied prior to submission. The raw reads are deposited as generated and linked to the existing BioSample accessions within BioProject PRJNA1142912.