PNM6
Library preparation and sequencing were outsourced to Novogene (Novogene Co., Ltd., Beijing, China). Briefly, 500 ng of total RNA were processed and after rRNA depletion, strand-specific libraries were generated with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) according to the suppliers protocol. Sequencing was performed on an Illumina platform using 150 bp paired end reads. Library size distribution and quality were verified using an Agilent 5400 Fragment Analyzer.