GSM8486571: Cp, ATAC-seq, developing forelimb, CS17, Rep1; Carollia perspicillata; ATAC-seq
Bat limbs were dissected and dissociated with trypsin. 50,000 cells per reaction were lysed and isolated nuclei were incubated with Tn5 Transposase (Illumina, #20034197) for transposition. DNA fragments were purified and barcoded adaptors were added via PCR. Fragments were purified and sequenced as 100 bp paired-end reads on a NovaSeq 6000 system. ATAC-seq experiments were performed in biological duplicates.