Spatial transcriptomics and proteomics of post-transplant HCC recurrence using 10x Visium CytAssist
Spatial transcriptomics and proteomics of post-transplant HCC recurrence using 10x Visium CytAssist
Human liver tumor samples were collected during surgical resection (primary and recurrent lesions). Sections (5 µm) were mounted onto 10x Genomics Visium slides (11 mm) using CytAssist, H&E stained, imaged, and stored at −80 °C. De-identified metadata (age, sex) were retained. No bulk extraction was performed. Poly-A RNA was captured in situ according to the 10x Genomics Visium manufacturer's instructions. For protein, oligo-conjugated antibodies were applied following the CytAssist Spatial Protein procedure, and excess reagents were removed by the recommended washes. Visium gene expression (GEX) libraries and Feature Barcode antibody-derived tag (ADT) libraries were prepared with 10x Genomics kits following the manufacturer's protocols. Libraries were quantified (Qubit) and quality-checked (Bioanalyzer/Tapestation) before pooling.