RNA-Seq of homo sapiens: CD4+ T cells
CD4+ T helper cells were isolated from PBMCs by negative selection using magnetic depletion of non-target cells (CD4+ T Cell Isolation Kit, Miltenyi Biotec Inc) and then lysed immediately. RNA and DNA were extracted at the same time (AllPrep protocol, Qiagen Inc). Libraries were prepared using the TruSeq total RNA Ribo Zero kit (Illumina Inc) and sequenced on the HISEQ 4000 using 151bp paired-end reads.