WGS Geobacillus stearothermophilus
end repair and addition of d'A 3' overhangs, T/A ligation. Magnetic bead purification and pooling, ligation of sequencing adapters using Kit 14 chemistry. Sequencing with R10.4.1 nanopore flow cell, basecalling done with sup 5.0.0 model on Dorardo v0.9.5. The lowest-quality 10% of reads were removed using Filtlong v0.2.1