DSS_1
DNA was extracted from samples, and those meeting quality control standards proceeded to library construction. Libraries were prepared by random DNA fragmentation followed by adapter ligation and PCR amplification. The finalized libraries were loaded onto a flow cell, where cluster generation was performed via bridge amplification. Sequencing was carried out using Illumina's Sequencing by Synthesis (SBS) technology, and raw data were generated as FASTQ files through Real Time Analysis (RTA) software.