GSM9347809: Arp8_WT_10min_BiolRep2; Saccharomyces cerevisiae; RNA-Seq
Total RNA was Trizol/chloroform-extracted, precipitated using isopropanol and resuspended in water. Before library preparation, RNA was treated with DNase I and purified using RNAClean XP beads. 1 µg DNaseI-treated RNA was subjected to rRNA depletion using the S. cerevisiae - specific riboPOOL kit (siTOOLs Biotech) according to the manufacturers protocol. Libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturers protocol.