GSM9462206: Early-stage filamentous C. albicans cells, untreated, biological replicate 4; Candida albicans; RNA-Seq
Early-stage filamentous C. albicans cells were thawed, refrozen in liquid nitrogen, and resuspended in lysis buffer in bashing lysis tubes, followed by mechanical disruption using a Tissuelyser LT (Qiagen) for 20 min at 50 Hz. C. auris pseudohyphae were ground for 1 min with nuclease-free sand using a microcentrifuge tube–compatible glass pestle, frozen again in liquid nitrogen, and reground in lysis buffer. Lysates were centrifuged (9,000 × g, 2 min), and total RNA was purified using the Quick-RNA Plant Miniprep Kit (Zymo Research, Tustin, CA, USA) according to the manufacturer's instructions. total RNA library preparation protocol for Illumina NovaSeq X Plus 2x150 nt: Novogene NGS library preparation kit