GSM9206719: ACHN Cells, Control replicate 2; Homo sapiens; RNA-Seq
Total RNA was isolated from cells using TRIzol reagent (Thermo Fisher Scientific, USA). RNA integrity was confirmed by Bioanalyzer prior to library construction. RNA libraries were prepared using the NEBNext Ultra Directional RNA-Seq Kit (New England BioLabs, Rowley, MA, USA) according to the manufacturer's protocol. Libraries were sequenced on the Illumina HiSeq 2500 platform to generate paired-end 100 bp reads.