GSM9461545: cycling non mito received-3; Homo sapiens; RNA-Seq
U87 cells from co-culture system were extracted and sorted using sorting flow cytometer (BD FACSAria Fusion) and washed with 1 × PBS for three times. RNA from various groups was extracted with TRIzol reagent. After RNA integrity was assessed, mRNA was purified using poly-T oligo attached magnetic beads and reverse transcribed into cDNA products using random hexamer primer and reverse transcriptase. Second strand cDNA was synthesized by DNA Polymerase I and dNTP. The library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA) and assessed library quality on the Agilent Bioanalyzer 2100 system, the clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumia). The library preparations were sequenced on an Illumina Novaseq platform and 150 bp paired-end reads were generated.