Bulk RNA-seq of of hemocytes in adult sugar-fed female Anopheles gambiae injected with dsRNA against Puckered
The aim is to investigate the transcriptional changes in the different hemocytes of adult Anopheles gambiae in response to activation and suppression of immune pathways leading to altered hemocyte differentiation and function. Bulk RNA sequencing was performed on fixed hemocytes collected from mosquitoes injected with control (dsLacZ injected) and dsRNA against immune pathway regulator genes (Cactus, Rel1,Jnk, Puckered, SOCS,STAT-A,Rel2 and Caspar) using the Illumina paired-end sequencing.