GSM8508557: A37_Male5; Drosophila melanogaster; RNA-Seq
For RNA extraction, eight mid-guts were dissected from gut-induced CrebB (TIGS>CrebB- plusRU486) flies, non-induced CrebB (TIGS>CrebB- minusRU486) flies, driver alone (TIGS only- plusRU486) and driver alone (TIGS only- minus RU486) flies at indicated times in ice-cold PBS and placed into ice-cold TRIzol (Thermo Fisher Scientific, 15596026). Five experimental replicates were performed and RNA samples were preserved in -80oC freezer prior to extraction by TRIzol/choloroform method as per manufacturer's instructions and quantified by NanoDrop 2000c spectrophotometer. For RNA sequencing, library preparation (Illumina, strand-specific RNA-seq with PolyA selection) and sequencing (Illumina NovaSeq 2 x 150 bp sequencing) were performed by Azenta Life Sciences (GENEWIZ UK Ltd., Essex, UK). 2x150 bp sequencing