GSM9135734: PDX2263_sgNeg_Lexogen_1; Homo sapiens; RNA-Seq
Total RNA was isolated from 0.5~5 million cells using Direct-zol RNA Miniprep Plus kit (ZYMO) or RNeasy Mini Kit (Qiagen) with DNase I treatment in column. RNA-seq libraries were prepared using QuantSeq 3' mRNA-seq Library Prep Kit (Lexogen) with 0.5-2 µg of total RNA according to the manufacturer's protocol.  Library quality was checked by High Sensitivity Bioanalyzer (Agilent) and libraries with different barcodes were pooled and sequenced on the Nextseq 500 platform with 75 bp single-end reads.