Capra hircus_Ovary_scRNA-seq
After resuspending the nuclei in suspension buffer, filter the solution through a 20 m cell strainer and collect the filtrate for counting. Gel beads containing barcode information are combined with a mixture of cells and enzymes, then encapsulated into droplets by oil-surfactant in a microfluidic system, forming GEMs (Gel Bead-in-Emulsions).