GSM9256654: R. macaque granuloma single-cell RNAseq, replicate 2 of Di5F animal, stimulated; Macaca mulatta; RNA-Seq
Granuloma samples were processed with the ScaleBio Low Input Fixation protocol for single cell RNA sequencing with the ScaleBio Sample Fixation Kit (Scale Biosciences #2020001) with additional fixation time required for BSL3 procedures. Fixed frozen samples were thawed on ice, counted, resuspended in Wash Buffer at 2000 cells per μL and processed with the ScaleBio Single cell RNA sequencing Kit v1.1 (Cat #950884) and laboratory protocol RevA NOV2023 from ScaleBiosciences. Approximately 10,000 cells were added per well to the RT-barcode plate on ice.