GSM9136295: hESC, d0, MECP2-WT, MECP2-R133C, MECP2-R168X, MECP2-R270X, L1; Homo sapiens; RNA-Seq
Human ESCs (WT, R133C, R168X and R270X) were harvested at the pluripotent stage, dissociated with Gentle Cell Dissociation (Stem Cell Technologies) and resuspended in ice-cold PBS + 0.04 % BSA. Two single-cell libraries were constructed from the four fixed samples using the EVERCODE™ WT Mini v2 Kit (Parse Biosciences, #ECW02115).