GSM9752408: scRNA-seq Pool-2 Gene Expression (aged murine dECM, MCF10DCIS.com); Homo sapiens; RNA-Seq
We used dead cell removal kit (Miltenyi, Cat. No: 130-090-101) to remove the dead cells. The cells were then labelled with human-HTO antibodies and sent to 10x genomics analysis.  L1 = Young mouse ECM implantation; L2 = Aged mouse ECM implantation 10X libraries were prepared using NextGEM single cell 3' library prepration kit with standard 10X genomics protocol. HTO library were constructed by following CITE-seq protocol.