GSM8486806: Aconitum_japonicum_Rootlet; Aconitum japonicum; RNA-Seq
Frozen samples of A. japonicum were powdered using a Multi Beads Shocker (Yasui Kikai, Japan) for subsequent RNA extraction. Total RNA for each of the four tissues was extracted using RNeasy Plant Mini Kit (Qiagen, USA), according to the manufacturer's instruction. cDNA library was then prepared using SureSelect Strand Specific RNA Library kit (Agilent 150 Technologies, USA) according to the manufacturer's specifications. mRNA was isolated from total RNA of each tissue using beads containing oligo(dt), which was then added with fragmentation buffer to shear mRNA into short fragments and used as a template for the synthesis of first strand cDNA synthesis using random hexamer primers. The cDNA library for Illumina sequencing was prepared using SureSelect Strand Specific RNA Library kit (Agilent, USA) according to the manufacturer's instruction.