tBE_TargetSeq_Sample_446:P2,5D,CD34+HSPC,HBG1/2-ON
PrimeSTAR HS DNA Polymerase was used to amplify the sequence of target site from the genomic DNA. PCR product was gel purified using FastPure Gel DNA Extraction Mini Kit. DNA library was prepared following the manufacturers instruction manual (NEBNext Ultra II FS DNA Library Prep Kit, NEB).