Amplicon-seq of Acropora millepora
DNA was extracted using the Maxwell RSC PureFood GMO and Authentication Kit (Promega no. AS1600). PCR was performed to amplify CRISPR-Cas9 target regions The resulting amplicons were then verified with gel electrophoresis and sequenced at AGRF in Brisbane using next-generation amplicon sequencing with the Illumina MiSeq system (150bp Paired End reads).