H2087-LCC2, H2087-TGL, SK-LC-17, LNCAP-EGFP, GA-10-Clone-4, H2087-LCC1, MDA-231-TGL, Sk-Mel-24, HL-60, SK-CO-01, HCC1954-LCC1, CAPAN02, co-processed cultured human cancer cell lines used for EasySci single-cell RNA sequencing
Nuclei isolated from cultured human cancer cell lines were processed using the EasySci-RNA protocol. Reverse transcription, ligation-based barcoding, and indexed PCR amplification generated single-cell transcriptome libraries, sequenced on an Illumina NextSeq 1000 (paired-end 100 bp; dual 10 bp index reads).