Nanopore barcoding of Acari and Collembola
Amplicon sequencing of 6,016 individually processed soil arthropod specimens, including 5,609 mites (Acari) and 407 springtails (Collembola), along with 128 negative controls. Each specimen was PCR-amplified using indexed primers targeting a ~650 bp fragment of the mitochondrial COI barcode region. Amplicons were subsequently pooled into a single library and sequenced on an Oxford Nanopore GridION platform (Oxford Nanopore Technologies). Basecalling was performed using Dorado, and reads are provided as a single FASTQ file prior to demultiplexing. Information required for demultiplexing and for linking sequences to individual specimens, including primer indices and specimen-level metadata, is available in Dryad (https://doi.org/10.5061/dryad.j9kd51ct5).