GSM9568385: Wollemia ovule Bisulfite-seq; Wollemia nobilis; Bisulfite-Seq
Genomic DNA from mature pollen and ovule DNA was isolated using CTAB buffer (2% CTAB, 100 mM Tris-HCl pH 8, 20 mM EDTA, 1.4 M NaCl, 2% PVP). For the bisulfite conversion, EZ- DNA Methylation Gold kit (Zymo Research) was used according to the manufacturer's instructions.  Converted DNA was then used for the library preparation using the NEXTFLEX Bisulfite-Seq kit (Perkin Elmer) and each library was sequenced on the NextSeq high output platform, paired-end 150-nt.