GSM9498208: Lung fibroblast, Control, Rep 3; Homo sapiens; RNA-Seq
RNA extraction was performed with GenUP Total RNA Kit (Biotechrabbit, Berlin, Germany), following the manufacturer's protocol. Total RNA was extracted from ZNF469-knockdown with or without doxycyclince (DOX) induction and then to Beijing Biomarker Technologies Co., Ltd. (Beijing, China) for transcriptomic sequencing. RNA quality and integrity were evaluated according to the BMKGENE standard workflow using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Sequncing libraries were constructed with the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs) and then, sequenced on an Illumina NovaSeq 6000 platform with paired-end 150 bp reads (Illumina, San Diego, CA, USA).