CK-2 (CRX897887)
After total RNA was extracted, eukaryotic mRNA was enriched by Oligo(dT) beads. Then the enriched mRNA was fragmented into short fragments using fragmentation buffer and reverse transcribed into cDNA with random primers. Second-strand cDNA was synthesized by DNA polymerase I, RNase H, dNTP and buffer. Then the libraries were constructed through end-repair, A-tailing, adapter ligation using a QiaQuick PCR extraction kit.