Ctr-3 (CRX1980825)
We conducted scRNA-seq analysis on testicular samples from control (n=3) and cmtr2 knockout (KO) (n=3) mice at postnatal day 12. Testes were digested in 2 mL GEXSCOPE Tissue Dissociation Solution using a Singleron PythoN Automated Tissue Dissociation System. Single-cell suspensions were loaded into microfluidic devices using a Singleron Matrix Single Cell Processing System. scRNA-seq libraries were constructed following the protocol of the GEXSCOPE Single Cell RNA Library Kit.