ssDNA library preparation for cfDNA from plasma sample S0162
1 ng of cfDNA was denatured and then SRSLY adapters were ligated as per the standard kit protocol except that the adapters were fully methyl conversion protected with 5mC modifications. Post adapter ligation and DNA purification the library molecules were methyl converted using the NEBNext Enzymatic Methyl-seq v2 Conversion Module according to manufacturers instructions. Post methyl conversion and purification the libraries were amplified for 11 cycles using NEBNext Q5U Master Mix and in house indexes.