Amplicon sequence of human fecal CZX_4386597 (CRX2091222)
Following collection, fecal samples were subjected to genomic DNA extraction. The purity and concentration of the extracted DNA were assessed by UV-Vis spectrophotometer. PCR amplification, construction of high-throughput sequencing libraries, and 16S rRNA sequencing were performed by LC-Bio Technology Co., Ltd. (Hangzhou, China). The V3-V4 hypervariable regions were amplified with polymerase chain reaction (PCR) using the primer pair 341F (5'-CCTACGGGNGGCWGCAG-3') and 805R (5'-GACTACHVGGGTATCTAATCC-3'). For data analysis, FastQC and MultiQC were employed to evaluate key sequencing metrics