HiC for Rousettus leschenaultii : adult male
Hi-C libraries were prepared from cross-linked nuclei using DpnII restriction enzyme digestion and proximity ligation following the standard protocol. The biotin-labeled ligation products were purified, sheared to ~400 bp, and captured with streptavidin beads. The final libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit and sequenced on the DNBSEQ-T7 platform.