GSM9754143: Exp7-Patients-PBMC-BM-1000000000361926-Tet-Lo, scFB; Homo sapiens; OTHER
Live, tetramer positive CD8 cells were sorted and run on 10X Genomics 5' Cell Immune Profiling workflow using manufacturer's instructions. Sorted cells were loaded onto a lane of a Chromium Next GEM Chip G for GEM production using the Chromium Controller from 10X Genomics. GEM-RT, cDNA amplification and cleanup were performed using Chromium Single Cell V(D)J Reagent Kits User Guide (v1.1 Chemistry) with Feature Barcoding technology for Cell Surface Protein (RevG) workflow per 10X Genomics protocols.