Shotgun metagenomic sequencing of Ovis aries: vaginal microbiota samples from multiparous ewes
Vaginal swabs was processed using the Real Vaginal Microbiome DNA Kit (Durviz S.L., Spain). DNA concentration and quality were measured with a Qubit fluorometer and Nanodrop spectrophotometer. Samples (400 ng DNA) were multiplexed in 13 runs with 24 barcodes each, including controls, and sequenced using adaptive sampling to exclude reads mapping to the Oar_rambouillet_v1.0 sheep reference genome