GSM9207136: SCI; Mus musculus; RNA-Seq
Single-cell suspensions were prepared from spinal cord injury sites at 7 dpi. Briefly, mice were anesthetized and perfused with ice-cold PBS. Spinal cord segments encompassing approximately 1 mm  of tissue centered on the injury epicenter were carefully dissected under a stereomicroscope. The tissue  samples were immediately transferred to ice-cold Hibernate-E medium (Thermo Fisher Scientific) supplemented with B27 and GlutaMAX to maintain cell viability during processing. For single-cell dissociation, the collected spinal cord segments were enzymatically digested using the Neural Tissue Dissociation Kit (Miltenyi Biotec) according to the manufacturer's protocol. The Matrix NEO™ Automated Single-Cell Library Construction System, which streamlines the process of single-cell RNA sequencing (scRNA-seq). This system utilizes NEO-CHIP microfluidic chips based on the Poisson distribution principle, allowing cells to naturally settle into micro-wells, ensuring that each well contains a single cell and thereby reducing doublet rates. The Matrix NEO™ system automates critical steps in the scRNA-seq workflow, including chip priming, single-cell partitioning, cell lysis, and mRNA capture, all initiated with a single click. This automation enhances experimental efficiency and reproducibility by minimizing manual intervention. By integrating the Matrix NEO™ system into your scRNA-seq workflow, you can achieve efficient and reproducible single-cell library construction, suitable for various applications including whole transcriptome profiling, immune repertoire profiling, and targeted gene expression analysis.