GSM9207190: HDF_NaNO2_1; Homo sapiens; RNA-Seq
Total RNA was isolated from cell lysates using the Direct-zol RNA Miniprep kit (Zymo Research, USA, #R2052) according to the manufacturer's instructions. About 800 ng of total RNA (for each group) was utilized for library construction employing the NЕВNext® Poly(А) mRNА Magnetiс Isоlatiоn Mоdulе alongside the NЕВNext® Ultra II™ Dirесtiоnal RNА Librаry Prер Kit for Illuminа (Nеw Еnglаnd Biоlаbs), following the manufacturer's guidelines.