GSM9535587: PBMC_OP_6; Homo sapiens; RNA-Seq
RNA was isolated from babies' PBMCs using the AllPrep DNA/RNA/miRNA Universal Kit (80224, Qiagen, Hilden Germany) following the manufacturer's protocol. RNA libraries were then prepared via poly(A) selection and reverse transcription into cDNA, followed by library quality assessment. Sequencing was performed using 150 bp paired-end reads on an Illumina PE150 platform. Quality of sequencing was assessed with fastqc (v0.12.0) and fastq files were mapped to the GRCh38 human reference genome with HISAT2.