RNA-Seq of Homo sapiens: HEK293T expressing CV-A14 2A variant, replicate 2
Total RNA was extracted from HEK-293T cells using TRIzol reagent (Invitrogen) with DNase I treatment. RNA quality was assessed by Nanodrop and agarose gel electrophoresis, and quantified by Qubit 3.0. Stranded bulk RNA-seq libraries were constructed using the KC-Digital Stranded mRNA Library Prep Kit for Illumina (Seqhealth, DR08502), which employs 8-base unique molecular identifiers (UMIs) to label pre-amplified cDNA molecules for PCR duplication removal. Libraries with inserts of 200500 bp were sequenced on a DNBSEQ-T7 sequencer (MGI Tech) in PE171 mode.