GSM9887351: Ada + IgG4_Donor B; Homo sapiens; RNA-Seq
Cells were dissolved in RLT buffer (Qiagen, Cat. 79216) with 1% 2-mercaptoethanol for RNA isolation. RNA was isolated using NucleoSpin RNA Plus kits (Takara, Cat. 740984.50). Poly-A enrichment (stranded mRNA) library construction was performed, and an average of 30 million paired end reads were sequenced per sample.