16S rRNA sequencing of rectal swabs from Zambian infants in a randomized trial of growth interventions
Rectal swab samples were collected using FecalSwab (Copan Diagnostics Inc., USA), DNA was extracted using Zymo Quick-DNA Fecal/Soil Microbe Miniprep Kit, and 16S rRNA libraries targeting the V3V4 region were prepared using Zymo Quick-16S Plus NGS Library Prep Kit with 341F and 806R primers.