GSM9206181: Tmprss2CreERT2, lsl-EYFP, replicate 1, RNAseq; Mus musculus; RNA-Seq
Total RNA was extracted from FACS sorted Epcam positive, CD24a positive and EYFP positive prostate cells with TRIzol reagent following the manufacture's instruction (#15596026, Invitrogen). RNAseq libraries were prepared using standard Illumina PolyA library preparation protocol. Next generation sequencing was performed by Illumina NovaSeq platform with paired-end of 100bp for 30-40 million reads. The sequence data were mapped to the mouse genome (mm10) using STAR