Genome_17978_ONT
Genomic DNA was purified from an overnight culture grown overnight at 37C and 250 rpm in 5 ml of Luria-Bertani liquid medium with the Genomic DNA purification kit (Thermo Fisher), following the manufacturer's instructions. After the evaluation of DNA quantity and quality, a long-read library was constructed with the Oxford Naopore Technology. Library was constructed with the Rapid Barcoding Kit 96 V14 (SQK-RBK 114.96). The reads were obtained using a PromethION P24 with a R10.4.1. flow cell device. The Basecalling algorith was ont-doradod-for-promethion v7.4.12 on super-accurate mode. The read quality control and adapters trimming was performed with MinKnow.