RNA-seq of Clogmia albipunctata: alignment control, LNC14
This embryo was prepared for dsRNA injection but was not injected. It is an alignment control. Two library preperations were made from this embryo. At the desired stage the embryo was homogenized in lysis buffer and a centrifugation step was performed to pellet the nuclei. The nuclei were used for ATAC-seq library preperation and the supernatant was used to isolate RNA for RNA-seq library preperation.RNA was isolated from the ATAC prep by Zymo kit R2030. The RNA-seq library was prepared by the Genomics Facility at the University of Chicago. cDNA was synthesized using Takara Smarter v4 kit following the Takara Ultra-Low Input protocol. Library preperation by Illumina Nextera XT following the manufacturers protocol.Single embryo ATAC-seq library preparations were done essential as described in Drosophila melanogaster (Blythe and Wieschaus 2016; Soluri et al. 2020).This entry is the RNA-seq library.