Illumina NovaSeq 6000 paired end sequencing
Libraries construction and whole-genome sequencing were performed by Illumina ADN Prep Kit (Illumina Technology) and a short unique identifier for the sequencing library using IDT for Illumina DNA/RNA UD Indexes Set A (96 Indexes-96 Samples Cat. 20091654). The quantity and quality of the libraries were evaluated using QUBIT 1x dsDNA HS 500rx (Thermofisher, Cat. Q33231) by Qubit 2.0 Fluorometer Invitrogen (Thermofisher) and their size distribution was assessed running an aliquot on a High Sensitivity D1000 ScreenTape (Agilent Technologies, Cat. 5067-5584) and High Sensitivity D1000 ScreenTape Buffer (Agilent Technologies, Cat. 5067-5585) by TapeStation 4150 system (Agilent Technologies). The sequencing was done using a Illumina NovaSeq 6000 S1 Reagent Kit v1.5 (300 cycles) (2x150 bp reads) on the NovaSeq6000 system (Illumina Technology) in HealthinCode-Valencia, Spain. Sequencing data is converted into raw data and demultiplexed for the analysis into FASTQ files.