GSM9464918: PT22_Tamoxifen_3_day_Rep1; Mus musculus; ATAC-seq
Samples were washed with PBS and nuclei were prepared using the NE-PER Nuclear and Cytoplasmic Extraction Reagents Kit (Invitrogen). Nuclei were resuspended in 50 μl of 1× TD buffer, then incubated with 2–2.5 μl Tn5 enzyme for 30 min at 37 ⁰C (DNA Library Preparation Kit, Nextera). Samples were immediately purified by Qiagen minElute column and PCR-amplified with the NEBNext High-Fidelity 2X PCR Master Mix (NEB). qPCR was used to determine the optimal PCR cycles to prevent over-amplification. The amplified library was further purified by Qiagen minElute column and SPRI beads (Beckman Coulter).