oral human mycobiome
The isolates of fungal DNA were amplified by PCR (T100 Thermal Cycler, BioRad, California, United States), with primers targeting the ITS-1 regions of the fungal rDNA gene (5' -> 3'; F: GTAAAAGTCGTAACAAGGTTTC 2 R: GTTCAAAGAyTCGATGATTCAC with attached overhang adapter sequences (F:TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG and R: GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG) added to the internal primer attached to the 5' end compatible with Illumina's recommendations to prepare ITS libraries [Strati F, 2016]. The obtained amplicons were used to prepare a genomic library for NGS sequencing in the MiSeq platform (Illumina, San Diego, California, United States) according to the Illumina protocol with modifications described by SrokaOleksiak et al. [SrokaOleksiak A 2020].