GSM9462190: NSC-male-wt_rep1; Mus musculus; RNA-Seq
RNA was extracted using the Zymo quick-RNA miniprep kit according to manufacturer's instructions Libraries were prepared using the Illumina TruSeq V2 stranded mRNA sample preparation kits from 500 ng total RNA as per manufacturer's instructions. Fragments above 200 bp were size-selected and cleaned up using AMPure XP magnetic beads. Final cDNA libraries were quantified using D1000 tape on the TapeStation (4200, Agilent Technologies) for sequencing on the Illumina Nextseq500 platform using 80 bp, paired-end reads.