16S rRNA gene sequencing: human blood metagenom
DNA was extracted from 200 L whole-blood sample with MACHEREY-NAGEL NucleoSpin Blood kit (Ref. 740951.50); 16S rRNA gene V3-V4 regions were amplified by PCR; NGS library was prepared with Illumina Nextera XT Index Kit v2 (Ref. 15052166). Sequencing was carried out Illumina MiSeq platform.