NGS of Homo sapiensbronchoalveolar lavage fluid
In the process of library construction, we first preprocess the samples to remove the host nucleic acid background, in order to reduce interference with subsequent sequencing. Subsequently, using artificially designed million level probes based on the principle of complementary base pairing, these probes can specifically bind to the target pathogen sequence. We achieved efficient enrichment of pathogen sequences by mixing, capturing, and eluting non target sequences with probes and samples.Furthermore, the captured pathogen sequences will undergo second-generation sequencing library construction. This step includes key steps such as DNA or RNA extraction, fragmentation, end effector repair, adapter addition, and purification to ensure that the final constructed sequencing library meets the requirements of high-throughput sequencing.