Normal WGS for A01
After PicoGreen quantification and quality control by Agilent TapeStation, 293-500 ng of genomic DNA was sheared using an LE220-plus Focused-ultrasonicator (Covaris catalog # 500569), and sequencing libraries were prepared using the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) with modifications. Briefly, libraries were subjected to a 0.5X size selection using aMPure XP beads (Beckman Coulter catalog # A63882) after post-ligation cleanup. Libraries were either not amplified by PCR and were pooled equivolume and quantitated based on their initial sequencing performance or were amplified with 5 cycles of PCR and pooled equimolar. Samples were run on a NovaSeq 6000 in a PE150 run using the NovaSeq 6000 SBS Kit and an S4 flow cell (Illumina).