GSM9256787: Hhp2; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol (Invitrogen) following the manufacturer's protocol. RNA libraries were prepared using the VVAHTS® Universal V8 RNA-seq Library Prep Kit for Illumina (NR605-0, Vazyme) and sequenced on the Illumina NovaSeq 6000 platform with a 150-bp paired-end sequencing strategy. Enrichment of mRNA, library construction, sequencing, and data analysis were performed on the Yunbios platform. Raw sequencing data were processed by Skewer (v0.2.2) and its quality was assessed with FastQC (v0.11.2). The sequencing reads had a length of 2×150 bp. Read indexing and mapping to the human reference genome (GRCh38/hg38) were performed using Hisat2 (v2.0.0), followed by transcriptome assembly using StringTie (v2.1.4). Gene expression levels were quantified using Fragments Per Kilobase of transcript per Million mapped reads (FPKM).