GSM9053659: PHF23 WT HeLa, input, rep2; Homo sapiens; ChIP-Seq
Crosslinked DNA was fragmented with sonication and IP with H3K27Ac and PHF23 antibody. The DNBSEQ ChIP-Seq library construction method and sequencing process are carried out according to the following steps:1. Take IPed-DNA and Input DNA for sample qualification; 2. DNA-end repair, 3'-dA overhang and adaptor ligation. 3. PCR amplification and size selection. 4. Library quality control; 5. Library circularization; 6. The library was amplified to make DNA nanoball (DNB); 7. Sequencing on DNBSEQ (DNBSEQ Technology) platform.