GSM9568509: Klebsiella pneumoniae ΔdksA mutant strain (ΔdksA), biological replicate 2; Klebsiella pneumoniae; RNA-Seq
Total RNA was extracted from Klebsiella pneumoniae using TRIzol reagent (Invitrogen, USA) following the manufacturer's protocol. Genomic DNA was removed by DNase I treatment. Sequencing libraries were constructed using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA, Cat# E7530L) following manufacturer's recommendations. Index codes were added to multiplex samples.