FL-3 (CRX2132319)
Total RNA was extracted from tissues using standard methods. After qualification and quantification with an Agilent 2102 bioanalyzer, mRNA was enriched using Oligo dT magnetic beads. Following fragmentation, first-strand cDNA was synthesized with random hexamer primers, and second-strand cDNA was synthesized using dUTP instead of dTTP. Strand-specific library construction was completed through end repair, A-tailing, adapter ligation, fragment selection, amplification, and purification