GSM9567558: Untreated biol rep 3; Homo sapiens; RNA-Seq
RNA was extracted from cells using a RNeasy Mini Kit (Qiagen). 400 ng of total RNA from control and AraC-treated cells or Pyronin-Y high and low cells were enriched using poly-T oligo-attached magnetic beads, fragmented, and reverse transcribed into cDNA. The cDNA libraries were constructed and sequenced on an Illumina NovaSeq platform, generating paired-end reads