GSM9207012: RNA_KOCOMP_CD45.1_02; Mus musculus; RNA-Seq
Cell sorting of fluorescent antibody-labeled cell suspensions was performed on FACSAria II (BD Biosciences) at the Cytometry & Cell Sorting Laboratory at NYUGSoM. 3.3-18.6x103 migDC were sorted per sample. migDC = Live singlets lineage- (CD3- TCR-β- CD19- NK1.1- NKp46- Siglec-F- Ly6G-) CD11c+ MHC-IIhi. RNA was extracted from sort-purified cells using the QIAGEN RNeasy Mini Kit and the accompanying RNase-Free DNase Set according to the manufacturer protocol. cDNA libraries were prepared using the low input Clontech SMART-Seq HT with Nxt HT kit according to the manufacturer protocol.