GSM8993845: iR_WTA_EX_1; Homo sapiens; RNA-Seq
Total RNA was extracted using the TRIzol reagent (Beyotime, Shanghai, China) according to the manufacturer's protocol. Transcriptome sequencing, extract the total RNA of the samples and digest the DNA with DNase, then enrich the eukaryotic mRNA with magnetic beads with Oligo(dT) (if it is prokaryotic, the mRNA will be enriched by removing the rRNA through the kit); add interrupting reagent to interrupt the mRNA into a short fragment, and then synthesize one-stranded cDNA by using a six-base random primer with the interrupted mRNA as a template. The purified double-stranded cDNA was then subjected to end repair, addition of A-tail and connection of sequencing junction, followed by fragment size selection, and finally PCR amplification; the constructed library was qualified by Agilent 2100 Bioanalyzer, and then sequenced by sequencer, producing 125bp of mRNA. After the constructed library was qualified by Agilent 2100 Bioanalyzer, sequencing was carried out using sequencer to produce 125bp or 150bp bipartite data.