Longread (CCS) sequencing by Sequel II
Genomic DNA was sheared with Diagenode's Megaruptor 2 system. SMRTbell prep kit 3.0 (PacBio) was used to prepare SMRTbell libraries according to the manufacturer's protocol. The AMPure PB beads (PacBio) size selection step cleaned the library and depleted DNA fragments shorter than 5 kb. The sizes of the libraries were checked by loading them on a Femto Pulse system (Agilent).