bacterial microbiome of Ulmus parvifolia  P07
Total DNA was extracted from 100 mg surface-sterilized cambium using a CTAB protocol. The V5-V6 region of the 16S rRNA genes was amplified by using primer set 799F and 1115R, which excludes chloroplast and mitochondria DNA of the host plant. The primers contained a heterogeneity spacer along with a barcode sequence.