RNA-Seq of iPSC-derived SMCs: rs11172113-associated enhancer deletion
Isolated total RNAs were quantified using Qubit ds DNA HS assay kit (Thermo Fisher Scientific, Waltham, MA, USA). PolyA+ mRNA libraries were prepared from 200ng-1g of total RNA using QIAseq Stranded RNA library preparation kit (Qiagen) according to manufacturers instruction.