GSM9377070: wsleaf, rep 2; Withania somnifera; RNA-Seq
100 mg wet weight of old leaf and berry tissue was harvested, 30 mg wet weight young leaf tissue, and 150-200 mg wet weight of root tissue was harvested. Harvested tissues were flash frozen in liquid nitrogen and ground to a fine powder in liquid nitrogen using a mortar and pestle. RNA was extracted using the Qiagen RNeasy Plant Mini Kit. Extracted RNA samples were flash frozen in liquid nitrogen and submitted to the Genome Technology Core at Whitehead Institute for Illumina paired-end RNA sequencing. Libraries were prepared for RNA-Seq using IDT's Swift Rapid RNA Library Prep Kit, according to manufacturer's directions.