ISO-seq of Wolffia brasiliensis clone 7522
Approximately 100 mg of plant material from W. brasiliensis was flash-frozen and ground to a fine powder using 1mm glass beads (Carl Roth, #A554.1) on a SilamatS6 (Ivoclar Vivadent). RNA was then extracted with the Rneasy Plant Mini Kit (Qiagen#74904) was used following manufacturers instructions. The library was prepared using the SMRTBell prep kit v2 (Pacific Biosciences) following instructions from the manufacturer