GSM8941792: Ctrl mouse2; Mus musculus; RNA-Seq
Cerebellum tissues were homogenized in 2 mL of ice-cold Nuclei EZ lysis buffer (MilliporeSigma, cat # EZ PREP NUC-101) using Dounce homogenizers. Nuclei were centrifuged at 500×g at 4°C for 5 min, washed with ice-cold Nuclei EZ lysis buffer. After wash, the nuclei were resuspended in nuclei suspension buffer (NSB) consisting of 1x PBS, 1% BSA (Thermo Fisher Scientific, cat# AM2618,) and 0.2U/l RNase inhibitor (Thermo Fisher Scientific, cat# AM2694) and were filtered through a 40-μm Flowmi Cell Strainer (Bel-Art, cat# H13680-0040). Cell suspension was adjusted to a final concentration of 1000 nuclei/μl with NSB. Single-nuclei RNA-seq libraries were prepared using the Chromium Single Cell 5' Reagent Kits (10x Genomics) according to the manufacturer's protocol.