GSM9533313: HBECs, Donor 2, Not Ya Son's Weed Cartridge Grandaddy Purp, Well 2; Homo sapiens; RNA-Seq
After 10 puffs, the basolateral medium was removed, and cells were lysed in 300 µL RNA Lysis Buffer with 2-Mercaptoethanol (10%) (Invitrogen, Waltham, MA) and stored at -80 °C. RNA was isolated from 16HBE cells and HBEC lysates using a PureLink RNA Mini Kit (Invitrogen) and all manufacturer's instructions were followed. Following preliminary quality assessment of RNA with a CLARIOstar and LVis plate (BMG LABTECH, Cary, NC), RNA was sent to GENEWIZ (Azenta Life Sciences, South Plainfield, NJ) where quality was further assessed with RNA ScreenTape Analysis and a TapeStation (Agilent, Santa Clara, CA). HBEC samples were sequenced on an Illumina Novaseq X Plus (2x150bp). The sequencing strategy utilized a paired-end run. Reads were mapped to the Homo sapiens GRCh38 with ERCC genes reference genome (ENSEMBL) 60. Regarding sample quality, HBECs had 1,266,194,279 total reads and a 38.54 quality score.