GSM8826309: SB1; Homo sapiens; miRNA-Seq
Total RNA was extracted using the miRNeasy Micro kit 3' and 5' adaptors were ligated to 3' and 5' end of small RNA, respectively. Then the first strand cDNA was synthesized after hybridazition with reverse transcription primer. The double-stranded cDNA library was generated through PCR enrichment. After purification and size selection, libraries with insertions between 18~40 bp were ready for sequencing. At least one of the samples has empty library name