EMseq of prunus dulcis: epicormic canopy tissue
Library concentration and quality was assessed using the Qubit 4 and Qubit 1X dsDNA HS Kit and by electrophoresis using a TapeStation. Libraries were pooled equimolarly, then cleaned using an equal volume of NEBNext Sample Purification Beads. The pool was then eluted in 25 l TE buffer and then concentration and DNA quality were revaluated using the above methods. The library pool was sequenced on two lanes of Illumina HiSeq 4000 platform to generate 150-bp paired-end reads.