GSM8717835: 8505c-DT; Homo sapiens; OTHER
After harvesting the cells from different groups, we extracted the genomic DNA. Library construction for NGS was performed by two-step PCR. The PCR products were purified and then sequenced on a HiSeq 2500 by Azenta Next Generation Sequencing Services. Each library was sequenced at 30–40 million reads to achieve ∼300 × average coverage over the CRISPR library. Library construction for NGS was performed by two-step PCR. The PCR products were purified and then sequenced on a HiSeq 2500 by Azenta Next Generation Sequencing Services. Each library was sequenced at 30–40 million reads to achieve ∼300 × average coverage over the CRISPR library.