GSM8506994: HCC,Cs+,3,P; Homo sapiens; ATAC-seq
ATAC-seq was performed as previously reported . Briefly, nucleus was extracted from samples,  and the nucleus pellet was resuspended in the Tn5 transposase reaction mix.The transposition  reaction was incubated at 37 ℃ for 30 min. Equimolar Adapter1 and Adatper 2 were added after transposition, PCR was then performed to amplify the library(ATAC-seq_Novogene Kit). After the  PCR reaction, libraries were purified with the AMPure beads and library quality was assessed with Qubit.