16S rRNA from konza graze soils
For library preparation, technical replicates were pooled, cleaned using Exo-SapIT (Applied BioSystems, Foster City, CA, USA), and quantified using the Quant-iT PicoGreen assay kit (Life Technologies, Grand Island, NY, USA). Samples were then combined into one library at a normalized amount of 100 ng per sample and finally cleaned via gel purification using the QIAquick Gel Extraction Kit (Qiagen, Germantown, MD, USA). Sequencing was performed at the Kansas State University Integrated Genomics facility on an Illumina MiSeq 2x150 PE cycle run, including a 15%PhiX spike.