GSM8486558: skinagetscha_S2_71years_scRNAseq; Homo sapiens; RNA-Seq
Epidermal samples were taken by the suction blister method.Subsequently epidermal sheets were enzymatically digested, using trypsin for 45 minutes at 37°C. The resulting single cell suspension was then passed through 100µm and 40µm nylon meshes and through a dead cell removal column before further processing. Single-cell RNA-seq was performed using the 10X Genomics Chromium Single Cell Controller with the Chromium Single Cell 3′ V3 Kit following the manufacturer's instructions.  After quality control, RNA sequencing was performed by the Biomedical Sequencing Core Facility of the Center for Molecular Medicine (Center for Molecular Medicine, Vienna, Austria) on an Illumina HiSeq 3000/4000 (Illumina, San Diego, CA, USA).