GSM9238109: Mouse, 6-OHDA+NDST3, rep1; Mus musculus; RNA-Seq
RNA-seq:Total RNA was isolated using an RNA extraction kit (Qiagen).A total of 1 µg of high-quality total RNA from each sample was utilized for library preparation following the manufacturer's protocol. CUT&RUN : The released DNA fragment were extracted using CUT&RUN Kit following manufacture's protocol. ATAC-seq : 50,000 nuclei were used transposition reaction using the Tagment DNA TDE1 Enzyme and Buffer Kit (Illumina) following manufacture's protocol. scRNA-seq : Tissue samples were enzymatically dissociated into single cell suspension using Multi Tissue Dissociation kit 1 (Miltenyi Biotec) in combination with the gentleMACS Dissociator, following the manufacturer's protocol. Post dissociation, myelin debris was removed via Percoll density gradient centrifugation, and red blood cells were eliminated using Red Blood Cell Lysis Solution (Miltenyi Biotec). Cell viability and concentration were assessed using the LUNA-FL™ Automated Fluorescence Cell Counter (logos biosystems). RNA-seq :TruSeq Stranded mRNA Library Prep Kit following manufacture's protocol. CUT&RUN : TruSeq DNA Sample prep Kit following manufacture's protocol. ATAC-seq : Manual ATAC-Seq library preparation involves separate cell lysis, Tn5 transposase tagmentation, DNA purification, and PCR amplification steps performed individually. scRNA-seq : Single-cell libraries were generated using the Chromium Next GEM Single Cell 3' v3.1 protocol (10x Genomics).