Mouse hepatocyte IgG CUT&Tag
After the library passes quality control, different libraries are pooled based on the effective concentration and the required amount of target data for sequencing. Illumina NovaSeq sequencing is then performed. The sequencing is based on the principle of Sequencing by Synthesis (SBS), where four types of fluorescently labeled dNTPs, DNA polymerase, and adapter primers are added to the sequencing flow cell for amplification. During the extension of each sequencing cluster, each addition of a fluorescently labeled dNTP releases a corresponding fluorescence signal. The sequencer captures this fluorescence and, through computer software, converts the light signals into sequencing peaks to obtain the sequence information of the target fragments.