Red Tide_Mock_B_7dpi
At 1 and 7 DPI with each bacterium or mock solution, two leaves of each plant (n = 3) were excised at the base and quickly processed for analyses. A piece of foliar tissue was used for bacterial population enumeration, and the rest were placed into a 2 mL tube and immediately frozen in liquid nitrogen. Tissue disruption, RNA extraction, and RNA quality assessment were carried out as described in detail by Jacob et al. (2021). High quality RNA was used for library preparation and sequencing (Illumina HiSeq 4000) by the DNA Technologies & Expression Analysis Core Laboratory at the University of California (UC), Davis, USA following a standard protocol for 3 tag-sequencing.