GSM9022978: Saline-Sedentary replicate5; Rattus norvegicus; RNA-Seq
Total RNA was extracted from 30mg of frozen medial gastrocnemius (left leg) of six samples per group using the RNeasy® Plus Universal mini kit (QIAGEN) following the manufacturer's instruction. Total RNA quality was assessed using the Agilent TapeStation, and all samples had RNA integrity numbers (RIN) greater than 8. RNA quantification was performed with the Qubit RNA BR Assay Kit Poly-A-based RNA-seq libraries were prepared using the Illumina Stranded mRNA Prep Kit with 650ng of RNA input per sample, following the manufacture's protocol. Library quality and size distribution (average size: 340-360bp) were confirmed using the Agilent TapeStation.