16S rRNA Gene Amplicon Sequencing of the Mice Fecal Microbiome treated or not with a Next-Generation Probiotics for Inflammatory Bowel Disease
PCR-grade water was used to prepare the 16S rRNA gene library targeting the V4 region, following the protocol described by the Schloss lab and Centeno-Martinez et al. (2022). The resulting 16S rRNA gene amplicons were then sequenced on the Illumina MiSeq platform using paired-end 2  250 bp reads.