16S rRNA sequencing of human: infant stool day 365
DNA was extracted using the QIAamp PowerFecal DNA Kit (QIAGEN). The V4-V5 region of the 16S rRNA gene was amplified using primers 515F (5-GTGYCAGCMGCCGCGGTAA-3) and 926R (5-CCGYCAATTYMTTTRAGTTT-3). Amplicons were normalized with the Just-a-Plate 96-well normalization kit (Charm Biotech).