GSM8459114: 3 samplespooled-CPL scRNA seq; Mus musculus; RNA-Seq
Yolk sac from E8.75 mouse embryos were dissected, disassocited into single cells and labelled with oligos (CellPlex kit) before pool loading to 10x Chips. Cells were captured in droplets. Following reverse transcription and cell barcoding in droplets, emulsions were broken and cDNA purified using Dynabeads MyOne SILANE followed by PCR amplification per manual instruction.  Finallibraries were sequenced on Illumina NovaSeq S4platform (R1 – 28cycles, i7 – 8cycles, R2 – 90 cycles).