Long-read metagenomic sequencing of WildR F7 gut microbiome: aliquot of pooled cecal contents
High molecular weight gDNA was extracted from an aliquot of the WildR F7 cryopreserved stock using a Qiagen Genomic-tip 20/G and cleaned following the protocol described here: PMID: 33277629. Libraries were prepared with the SQK-LSK110 kit and selecting all fragments >3kb. Sequencing on a R9.4.1 SpotOn flow cell was performed until ~50 pores remained (74 hours).