16S rRNA V3-V4 amplicon from symptomatic tomato rhizosphere sample, block 1
Total soil DNA was extracted using the DNeasy PowerSoil Kit according to the manufacturer's protocol. The V3-V4 region of the bacterial 16S rRNA gene was amplified using primers Bakt_341F (5'-CCTACGGGNGGCWGCAG-3') and Bakt_805R (5'-GACTACHVGGGTATCTAATCC-3'). Sequencing was performed on an Illumina NovaSeq platform using 2 x 250 bp paired-end chemistry by Novogene Inc. Reads were demultiplexed by sample, merged, and quality filtered. The submitted FASTQ files contain the resulting effective tags with per-base quality scores.