GSM9138210: Tumor-infiltrating immune cells, creatine-treated; Mus musculus; RNA-Seq
Day 14 B16-OVA tumors were harvested from experimental mice to prepare TII suspensions (4 tumors were combined for each group). TII suspensions were then sorted using a FACSAria II flow cytometer to purify immune cells (gated as DAPI−CD45.2+ cells). Purified TIIs were quantified using a Cell Countess II automated cell counter (Invitrogen/Thermo Fisher Scientific). A total of 10,000 TIIs from each experimental group were loaded on the Chromium platform (10x Genomics), and libraries were constructed using the Chromium Single Cell 3′Library & Gel Bead Kit v2 (catalog no. PN-120237, 10x Genomics) according to the manufacturer's instructions.