GSM8827825: 2205; Mus musculus; RNA-Seq
Total RNA was isolated from tumors using the RNeasy Mini Kit (#74104, Qiagen) according to the manufacturer's instructions for all (N = 19) fresh-frozen samples. The integrity of the RNA was assessed using Agilent TapeStation, and samples with RIN > 6.0 were used. Library prep was conducted at Azenta. mRNA-seq libraries for the Illumina platform were generated and sequenced at Azenta using the Illumina HiSeq 2 × 150 bp configuration following the manufacturer's protocol. ~350M raw paired-end reads per lane.