Single-nucleotide variant and marker frequency analysis of Sinorhizobium meliloti strains with rearranged genomic DNA
Single-nucleotide variant and marker frequency analysis of Sinorhizobium meliloti strains with rearranged genomic DNA
Cells were harvested by centrifugation at 3000 x g and 4Â° C S. meliloti cells were grown in TY supplemented with appropriate antibiotics to an optical density 600 (OD600) of 1.0 (Exp.) or 2.0 (Stat.). Total DNA for resequencing was extracted using a DNeasy Blood and Tissue kit (Qiagen) according to the manual. DNA libraries for sequencing were generated by applying a Nextera XT DNA Library Preparation kit (Illumina) or Ligation Sequencing Kit (Oxford Nanopore).