Model organism or animal sample from Sus scrofa
After extracting the genomic DNA from the samples, the V3 + V4 region of 16S rDNA was amplified using specific primers with barcodes. The primer sequences were: 341F: CCTACGGGNGGCWGCAG; 806R: GGACTACHVGGGTATCTAAT. The purified amplification products (i.e., the amplicons) were ligated with sequencing adapters to construct a sequencing library, which was then sequenced on the Illumina platform.