RNA-Seq of AR-GBS
Firstly, the total RNA of the sample was extracted by the Trizol method, and the sample quality inspection was conducted using Thermo NanoDrop One and Agilent 4200 Tape Station. After passing the detection, the ribosomal RNA was removed using the Epicentre Ribo-Zero rRNA Removal Kit. Strand specific libraries were constructed using the NEBNext Ultra IIDirectional RNA Library Prep Kit for Illumina.