Illumina NovaSeq 6000 paired-end Sequencing of KAS22012565 (KAE22133650)
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Genomic DNA gDNA with high molecular weight was extracted from frozen leaves, and the quality of gDNA was confirmed by spectrophotometric analysis DS-11 Spectrophotometer; DeNovix Inc. and agarose gel electrophoresis 1.0% w/v agarose TAE 1X gel containing 1X EcoDye; BIOFACT, Daejeon, Korea. The paired-end PE libraries for NGS were constructed using the TruSeq DNA Nano Kit for 550 bp insert sizes. The quality of each library was validated by qPCR. PE libraries were sequenced with the NovaSeq6000 sequencing platformsIllumina.