GSM9682424: C3H10T1/2 wiltype (WT) cells (unedited); Mus musculus; RNA-Seq
Total RNA was extracted from approximately 1 million cells using AllPrep DNA/RNA/miRNA Universal Kit (QIAGEN) according to the manufacturer's instructions, including on-column DNase I treatment. RNA quality was assessed using an Agilent 2100 Bioanalyzer prior to library preparation. RNA libraries for RNA-seq were generated using the TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat (Illumina) according to the manufacturer's instructions.