16S rRNA Gene Amplicon Sequencing of the Mice Fecal Microbiome treated or not with a Next-Generation Probiotics for Inflammatory Bowel Disease
Empy tubes were submitted to the same 16S rRNA gene library targeting the V4 region as controls, following the protocol described by the Schloss lab and Centeno-Martinez et al. (2022). The resulting 16S rRNA gene amplicons were then sequenced on the Illumina MiSeq platform using paired-end 2  250 bp reads.