GSM8684039: EM CD4 donor 2; Homo sapiens; RNA-Seq
1,000-10,000 T cells were flow sorted based on CD45RA and CCR7 expression into RPMI supplemented with 10% FBS.  RNA was then obtained use Qiagen DNA/RNA mini allprep kit. cDNA libraries prepared using TakaraBio's SMART-Seq v4 Ultra Low Input RNA Kit with Illumina's Nextera XT DNA Library Preparation Kit