Libraries were constructed from submitted RNA samples using a KAPA Stranded RNA-Seq Kit (REF KK8401), at a third volume of manufacturer's instructions, and using XGen Normalase UDI primers to incorporate indexes into libraries. Resulting libraries were equimolarly pooled based on DNA concentrations determined through photospectropic measures of SYBR green and on average fragment size as determined by an Advanced Analytical Fragment Analyzer. The final pool was quantified with Qubit version 4 and using qPCR (KAPA Library Quant Kit, REF 07960298001), which was performed on a Roche LightCycler 48