2,3-Butanediol producing bacillus genomic sequences
A sequence of nucleotides incorporated by the DNA polymerase while reading a template, such as a circular SMRTbell (TM) template. Polymerase reads are most useful for quality control of the instrument run. Polymerase read metrics primarily reflect movie length and other run parameters rather than insert size distribution. Polymerase reads are trimmed to include only the high quality region; they include sequences from adapters; and can further include sequence from multiple passes around a circular template.To get high quality data, adapter sequences were trimmed from the polymerase reads, and highly accurate (99% accuracy or more) consensus sequences are generated by merging adapter trimmed reads yielding from a same ZMW. The consensus sequences are called as HiFi (High Fidelity) reads. The HiFi reads have advantages to identify variants from single nucleotide to structural variant and to perform de novo assembly.