Targeted amplicon sequencing of Osteosarcoma patients
DNA from Osteosarcoma patients and Healthy Donor were extracted using using DNAzol. DNA quality was evaluated by Implen-GO NanoPhotometer N50-GO. Targeted amplicon library synthesis was performed using the Archer VariantPlex solid tumor panel Kit  following manufacturer instructions.  Prior to sequencing, the libraries underwent electrophoretic control on DNA1000 capillary electrophoresis cartridges of Bioanalyzer 2100  and quantification by Qubit fluorimetric DNA High Sensitivity Assay. The libraries have been sequenced in 2x 150bp paired-end and  run on a NextSeq 500 Illumina platform.