GSM9464953: sort-1, for sucrose gradient; Mus musculus; RNA-Seq
Repli-g WTA single cell kit was used for mRNA extraction or total RNA extraction, according to the manufacturer's protocols. The major steps included extraction and amplification of RNA and reverse transcription into cDNA for storage. A small pellet from the crude preparation (containing around 15 million particles) was resuspended in 10 µL of PBS, and the sorted synaptosomes (containing ~30 million particles) were resuspended in 10 µL of TCEP. The cDNA library (mRNA) was constructed using the 10x Genomics platform following the manufacturer's protocol and sequenced on an Illumina Hiseq 2000 in paired-end mode.The cDNA library (total RNA) was constructed using TruSeq Nano DNA Library Prep Kit following the manufacturer's protocol and sequenced on an Illumina NovaSeq X Plus in paired-end mode.