Transcriptomic landscape of the hippocampus in 2- and 4-month-old rTg4510 mice and wild-type littermates
Transcriptomic landscape of the hippocampus in 2- and 4-month-old rTg4510 mice and wild-type littermates
Hippocampi were dissected from 2- and 4-month-old wild-type and rTg4510 mice and snap-frozen (or stored at −80°C) until RNA extraction. Both male and female mice were allocated randomly between experimental groups.  Hippocampal formation was dissected from mouse brains and processed for total RNA extraction. Mice were maintained under standard laboratory housing conditions with ad libitum access to food and water. No treatment was applied; animals were maintained without pharmacological intervention. Total RNA was extracted using Trizol/Qiazol followed by purification with the RNeasy Mini Kit (Qiagen). Hippocampal tissue was lysed and homogenized in Trizol using a rotor-station homogenizer. Chloroform was added, samples were centrifuged (12,000 g, 10 min), and the aqueous phase was transferred to a new tube. Ethanol was added and samples were loaded onto RNeasy spin columns and processed according to the manufacturer's instructions. RNA was eluted in RNase-free water and either stored at −80°C or quantified immediately. Total RNA and library integrity were verified on LabChip GX Touch 24. Between 197.97 and 276.9 ng of total RNA were used as input for the SMARTer Stranded Total RNA Sample Prep Kit – HI Mammalian (Clontech/Takara), following the manufacturer's instructions.