Bulk K562 cells negative control (QC) for ChronoSeq with no stimulation, all 12 Time-Tags
Bulk K562 cells negative control (QC) for ChronoSeq with no stimulation, all 12 Time-Tags
Cells were automatically samples using the ChronoSeq device. 10μl of Time-Tagged beads at 450 beads/μl suspended in lysis buffer were directly mixed with human cells at 600cells/μl. In order 1->12 K562 grown in suspension in DMEM with 10% FBS and 1%PEN-STREP at 5%CO2 and 37C.  https://kanishkasthana.github.io/ChronoSeq/protocol_for_preparing_cells_AdditionalFiltration.html Oligo-DT beads were used to capture mRNA from lysed Single-Cells inside droplets. Beads are washed once with 6X SSC buffer and then twice with 1.25X RT buffer. Beads were then pooled together for a single RT reaction. very similar to Drop-seq. https://kanishkasthana.github.io/ChronoSeq/protocol_library_preparation_for_dropseq_chronoseq_beads.html