16S rRNA sequencing of metagenome: bamboo box container
Genomic DNA was extracted from environmental samples associated with ancient bamboo artifacts. The eukaryotic 18S rRNA gene was amplified using universal eukaryotic primers targeting a hypervariable region. The amplicons were purified and sequencing libraries were constructed with Illumina-compatible adapters and sample-specific barcodes. The final library was sequenced on an Illumina platform for paired-end reads.