GSM8828429: IE1_deltaCTD Rep3; Homo sapiens; RNA-Seq
RNA was harvested using New England BioLabs Monarch® Total RNA Miniprep Kit (T2010S) as per kit instructions. RNA was quantified by Nanodrop and integrity was analyzed with the 4200 Tapestation Bioanalyzer system (Agilent). 500 ng of total RNA with an RNA Integrity Number (RIN) >9.5 were used to prepare sequencing libraries with the TruSeq Stranded mRNA Library Prep Kit (20020594; Illumina). Library concentrations were measured with Qubit dsDNA HS Assay Kit (Q32854; Thermo Fisher Scientific) and then analyzed with Agilent High Sensitivity D5000 ScreenTape System and pooled. Libraries were sequenced with 100-bp paired-end reads on an  NovaSeq X+ 10B flow cell  at the Fred Hutch Genomics Core Facility.