GSM9351316: pre-onset phase NC1; Mus musculus; RNA-Seq
Liver tissue was perfused via the portal vein with cold PBS to remove blood, then dissected and snap-frozen in liquid nitrogen. Total RNA was extracted using TRIzol reagent (Invitrogen) with on-column DNase I treatment, followed by quality control: A260/A280 1.8–2.1, A260/A230 ≥ 2.0, RIN ≥ 7.0. Liver transcriptome profiling was conducted at two distinct timepoints during EAE progression. For the first analysis, total RNA was extracted from liver tissues collected at theEAE peak phase (approximately 15 days post-immunization, dpi) and compared with baseline controls (pre-immunization). Libraries were constructed using the Illumina NEBNext® Ultra™ RNA Library Prep Kit following the manufacturer's protocol. Qualified libraries were pooled and subjected to 150-bp paired-end sequencing on an Illumina NovaSeq 6000 platform. The second transcriptomic study focused on the EAE pre-onset phase (approximately 5 dpi), again comparing livers with baseline controls. RNA extraction was followed by library preparation using the VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina. Library quality was assessed using an Agilent 2200 system, and sequencing was performed on a DNBSEQ-T7 platform with a 150-bp paired-end configuration.