GSM9570578: HNE_unedited_1; Homo sapiens; RNA-Seq
Fully differentiated HNEs were incubated in accutase/trypsin solution at 37ºC for 7 minutes then resuspended. Cells were centrifuged, washed and re-suspended in PBS, with a portion stained with a viability dye for scRNASeq library prep. Library preparation was performed in the Cutting lab using 10X Genomics v.3.1 30 Library Preparation chemistry with a dual indexing system.