OG1RF ebpC::Tn resequencing
Illumina sequencing libraries were prepared using the tagmentation-based and PCR-basedIllumina DNA Prep kit and custom IDT 10bp unique dual indices (UDI) with a target insert size of280 bp. No additional DNA fragmentation or size selection steps were performed. Illuminasequencing was performed on an Illumina NovaSeq X Plus sequencer in one or more multiplexedshared-flow-cell runs, producing 2x151bp paired-end reads. Demultiplexing, quality control andadapter trimming was performed with bcl-convert1(v4.2.4). Sequencing statistics are included inthe DNA Sequencing Stats.xlsx file.