GSM9466326: WT mouse iGC B cells replicates 2, ATAC; Mus musculus; ATAC-seq
Approximately 1×10^5 cultured iGCB cells were harvested, washed once with 500 μL Wash Buffer. The nuclei were collected and subjected to a transposition reaction in a preheated metal bath at 37 °C. The DNA fragments were extracted and purified by ATAC DNA Extract Beads. Libraries were constructed using the TruePrep Index Kit V2(Vazyme) for sequencing.After quality inspection, the DNA libraries were sequenced on the Illumina PE150 platform to a depth of 10 G per sample by GENEWIZ.