GSM8685117: ATAC_E11.5Pdx1H_1; Mus musculus; ATAC-seq
Bulk-cell ATAC-seq was conducted following the published protocol  with modifications in cell sorting and nuclei preparation. Specifically, 2,000 cells per ATAC-seq were directly sorted into 50 ul lysis buffer in a 200 ul tube for nuclei preparation. Libraries were constructed using the TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme, TD502). PCR amplification was performed for 12 cycles under the following conditions: 72°C for 3 minutes, 98°C for 30 seconds, then 12 cycles of 98°C for 15 seconds, 60°C for 30 seconds, and 72°C for 3 minutes, followed by a final extension at 72°C for 5 minutes. The final DNA was purified using 1× VAHTS DNA Clean Beads and sequenced as 150 bp paired-end reads on the Illumina NovaSeq 6000 platform.