Genome sequencing of D178081
The experimental procedure was performed according to the standard protocol provided by Oxford Nanopore Technologies (ONT), including sample quality control, library preparation, library quality assessment, and library sequencing. The main steps are as follows:High-quality genomic DNA extraction, followed by purity, concentration, and integrity assessment using Nanodrop, Qubit, and 0.35% agarose gel electrophoresis.Large DNA fragment recovery using the BluePippin automated nucleic acid size selection system.Library preparation (SQK-LSK109 Ligation Sequencing Kit):DNA damage repair and end repair, followed by bead-based purification.Adapter ligation, followed by bead-based purification.Library quantification using Qubit.Sequencing on the ONT platform.