RNA-seq of eukaryotic plankton community
Plankton biomass from seawater was collected on 47mm 5m filters from shipboard incubation experiments (LT1 and ST1B) and in situ plankton communities at two light depths - 40% PAR (IS40) and 10% PAR (IS10) - during  the Export Processes in the Ocean from RemoTe Sensing (EXPORTS) North Atlantic field campaign. Total RNA was extracted using the Qiagen RNeasy mini kit with added bead beating and DNase steps, which was then sequenced at the University of California Davis Genome Center using polyA selection to bias results toward eukaryotic mRNA.