GSM8573304: EM-seq, whole adult; Paraspadella gotoi; OTHER
100 ng of P. gotoi gDNA were spiked in with unmethylated lambda phage and CpG methylated pUC19 control before fragmenting it to ~300 bp with a BioRuptor (Covaris). Resulting sheared DNA was then used to prepare an Enzymatic Methyl-Seq library according to the manufacturer's instructions (NEBNext Enzymatic Methyl-seq Kit).