sequences of gene
Use the RiboCop rRNA Depletion Kit for Mixed Bacterial Samples (lexogen, USA) to remove rRNA, then use mRNA as the template for reverse transcription to synthesize double-stranded cDNA, ensuring that the library only contains the first strand of cDNA. Construct the RNA library using Illumina's Illumina8 Stranded mRNA Prep and Ligation. Perform RNA-seq dual-end sequencing using the Illumina NovaSeq6000.