GSM9822948: tumor cell from control tumor, CR, replicate 1; Mus musculus; ATAC-seq
For LM3 samples, cultured cells were collected, washed with ice-cold phosphate-buffered saline, and lysed in cold lysis buffer to release nuclei. For mouse tumor samples, sorted GFP-positive tumor cells were washed with ice-cold phosphate-buffered saline and lysed in cold lysis buffer to release nuclei. Approximately 50,000 cells were used for each sample. Nuclei were collected by centrifugation and subjected to Tn5 transposase-mediated tagmentation. ATAC-seq libraries were constructed using a Tn5 transposase-based library preparation kit (Vazyme Biotech, TD711). Isolated nuclei were incubated with the transposition reaction mixture at 37°C for 30 minutes. Tagmented DNA was purified, PCR-amplified, and subjected to high-throughput sequencing on an Illumina platform.