GSM9678285: Input to ARID1A/PBRM1 ChIP in SUM159 ARID1A HET clone 77; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 min in 37 C. The reaction was quenched with glycine. Nuclei were isolated with a Triton-X-100-containing buffer, and chromatin was subject to sonication with BioRuptor Pico (Diagenode). After immunoprecipitation and reversal of crosslinking, the DNA was isolated with the phenol-chloroform method, and subject to library preparation. Ultra II DNA Library Prep Kit for Illumina, with dual indexes. Size distribution of the libarries was assessed by Agilent Bioanalyzer.