GSM8828042: MITDMY01; Homo sapiens; RNA-Seq
Stromal cells were specifically isolated via laser capture microdissection (LCM) of histologically stained (hematoxylin) mouse tumor sections before lysed for RNA preparation. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 μg of total amplified RNA for the construction of sequencing libraries per sample. RNA libraries were prepared for sequencing using standard Illumina protocols. Three independent libraries were generated from 3 individual samples per treatment group.