shotgun metagenomics sequencing of healthy human fecal sample DFI0032
Fecal samples should be aliquoted within 24 hours into 1.5 ml freezer vials and stored at -80C.  DNA extraction is performed using the QIAamp PowerFecal Pro DNA Kit and Illumina compatible libraries are generated using the QIAseq FX Library Kit. Sequencing runs are performed on the Illumina NextSeq platform in the Functional Genomics Facility at UChicago using the 2_150 Paired End reads cassette.