RNAseq of heart tissue from patients with diabetes vs normal heart tissue
RNAseq of heart tissue from patients with diabetes vs normal heart tissue
The hearts were collected and snap-frozen in liquid nitrogen at Baylor College of Medicine under proper licence and consent agreement; then imported under proper MTA and stored in -80 degree freezer with HTA licence before usage.  Total RNA from the heart tissue was extracted using TRIzol and treated with DNAse (ThermoFisher Scientific, AM1906) to eliminate genomic DNA contamination. The quality and integrity of total RNA samples were checked using a 2100 Bioanalyzer (Agilent Technologies). RNA samples were quantified using Qubit 4.0 Fluorometer (Life Technologies, Carlsbad, CA, USA) and RNA integrity was checked with RNA Kit on Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA). RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina following manufacturerâ€™s instructions (NEB, Ipswich, MA, USA).Sequencing libraries were validated using NGS Kit on the Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA), and quantified by using Qubit 4.0 Fluorometer (Invitrogen, Carlsbad, CA). 