GSM9756168: CD45 positive immune cells from melanoma tumors during targeted therapy–induced regression; Mus musculus; RNA-Seq
Tumors were mechanically and enzymatically dissociated into single-cell suspensions. CD45⁺ immune cells were isolated using magnetic bead enrichment and filtered through a 40 μm strainer. Cell viability was confirmed prior to library preparation. Single-cell libraries were generated using the 10x Genomics Chromium Next GEM Single Cell 3′ RNA-Seq Assay v3.1 (Dual Index) according to the manufacturer's protocol (CG000416, RevC). Libraries were sequenced on an Illumina NovaSeq 6000 instrument.