Illumina sequencing of Acinetobacter baumannii:398-StAb1eA
Samples were prepared for whole genome sequencing using the Illumina DNA Prep tagmentation kit and Illumina Unique Dual Indexes. Sequencing was performed on the Illumina NextSeq2000 platform using a 300 cycle flow cell kit to produce 2x150bp paired reads. 1-2% PhiX control was spiked into the run to support optimal base calling. Read demultiplexing, read trimming, and run analytics were performed using DRAGEN v4.2.7, an on-board analysis software on the NextSeq2000. We include fastqc metrics as a best practice and for examination in the case of unexpected outputs.