GSM8826821: SMA scramble N3 replicate 2; Homo sapiens; RNA-Seq
Total RNA was extracted using the ReliaPrep RNA Cell Miniprep System (Promega, Z6010). Libraries were prepared starting from 50 ng of total RNA derived from 9 control, 6 SMA, and 16 morpholino-treated SMA (8 r6-MO, 8 r6-MO-scrambled) organoids, using the SMARTer Stranded RNA-Seq Kit, TaKaRa) and following the manufacturer's instructions. Samples underwent a high coverage paired-end 150-bp strand-specific sequencing using a NextSeq 2000 platform (Illumina).