GSM9019995: Whole heart, postnatal day 56; Mus musculus; OTHER
Hearts of mice at P0, P7 and P56 stages were sacrificed, followed by direct dissection. Hearts were cleaned with cooled 1× PBS buffer and dried with Kimwipes. Subsequently, all heart tissue samples were rapidly frozen in liquid nitrogen before being embedded in Tissue-Tek OCT. Coronal cryosections with a thickness of 10 μm were then prepared using a Leica CM1950 cryostat. Finally, these tissue sections were affixed to Stereo-seq chips for subsequent experimental procedures. The spatially designated probe located on the surface of the STOmics chip was capable of capturing the mRNA molecules that were released. Subsequently, a reverse-transcription process was carried out, and the resulting cDNA product was released. cDNA is amplified by PCR, digested by enzymes, screened, reamplified, looped, and constructed into a standard library for DNBSEQTM sequencing.