16S rRNA amplicon sequencing of soil metagenome: humus derived from fermented soybean dregs compost
Microbial diversity analysis was performed on the Illumina NovaSeq sequencing platform, and small-fragment libraries were constructed using the paired-end strategy. For bacteria, total genomic DNA extracted from samples was used as the template, and the V3-V4 hypervariable region of the bacterial 16S rDNA was target-amplified with specific forward primer 338F (5'-ACTCCTACGGGAGGCAGCA-3') and reverse primer 806R (5'-GGACTACHVGGGTWTCTAAT-48').