RNA seq of Sphingobium RSMS
Total bacterial RNA was extracted and purified using Trizol (Sigma) and RNeasy Mini Kit (Qiagen). The concentration of extracted RNA was measured using Qubit RNA high sensitivity assay kit (Invitrogen). The samples having RNA integrity number (RIN value) >8 were used for the library preparation. A stranded library was constructed with NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) following manufacturers protocol.