GSM9350188: KO_1; Mus musculus; RNA-Seq
Total RNA from WT and GαqKO mouse embrionary fibroblasts extracted and purified with RNeasy Mini Kit (Qiagen), and genomic DNA was removed using the RNase-Free DNase Set (Qiagen). cDNA library was constructed using Stranded mRNA Library Preparation Kit and sequenced in the Illumina Novaseq platform, using 150bp paired-end sequencing reads.