GSM8967841: T cell differentiation model, experiment 1 – day 10; Mus musculus; RNA-Seq
CD45⁺ Cas9-LPs, with or without Notch stimulation, were sorted using a FACSAria (BD) and resuspended in PBS supplemented with 0.5% BSA. Single-cell RNA-seq libraries were generated using the 10× Genomics Chromium Single Cell 3′ Reagent Kit, following the manufacturer's protocol. Cells were encapsulated into GEMs, reverse transcribed, barcoded with cell-specific barcodes and UMIs, and cDNA was purified, amplified, and quality-checked. Libraries were constructed through enzymatic fragmentation, adapter ligation, indexing PCR, and double-sided size selection. Sequencing was performed on an Illumina NovaSeq platform, and data were processed with Cell Ranger for alignment to the mm10 genome, UMI counting, and expression quantification.