Chromosome conformation capture of Acipenser baerii
Hi-C data was generated using the Arima-HiC kit (Ref. 510008), according to the manufacturer's protocols using 100l of blood as starting material, the Truseq DNA PCR-Free kit and Truseq DNA UD Indexes (Illumina, ref. 20015962, ref. 20020590) and the KAPA library Amplification kit (Roche, ref. KK2620). Hi-C library was sequenced in paired end 2*150nt mode on Novaseq6000 (Illumina), using half a lane of a SP flow cell (ref. 20027464). Image analyses and base calling were performed using the Illumina NovaSeq Control Software and Real-Time Analysis component (v3.4.4). Demultiplexing was performed using Illumina's conversion software (bcl2fastq v2.20). The quality of the raw data was assessed using FastQC (v0.11.8) from the Babraham Institute and the Illumina software SAV (Sequencing Analysis Viewer). Potential contaminants were investigated with the FastQ Screen (v0.14.0) software from the Babraham Institute (Wingett & Andrews, 2018)