GSM9138147: scRNA_Untreated_GEX_lib_3mice; Mus musculus; RNA-Seq
Tumours were excised from untreated group and BUP-Gel treated mice and harvested in cold 1X PBS. Tumours were finely chopped, homogenized, and subjected to enzymatic digestion using collagenase IV and DNase I for 45 minutes at 37 C on constant shaking. Tumor suspension was further strained using cell strainers and single cells were obtained.These cells were stained for immune cells using anti-CD45 antibody followed by live dead staining. Sorting of live CD45+ and CD45- cells were done using FACS. CD45+ and CD45- cells were pooled in 4:1 ratio and run on seperate lanes for untreated and treated conditions seperately.