RNA-Seq of Cassia fistula: stems of mature tree, replicate 1
RNA was isolated with the Total RNA Purification Kit (Jena Bioscience, Dortmund, DTM, Germany). RNA concentration and integrity were measured using a NanoDrop 2000c UV-Vis Spectrophotometer (Thermo Scientific, Wilmington, DE, USA), a 2100 Bioanalyzer system (Agilent Technologies, Palo Alto, CA, USA), and agarose gel electrophoresis; RIN values ranged from 7.8 to 9.2. High-quality RNA samples (three biological replicates per organ) were processed with TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA, USA).