16S amplicon sequencing of soybean rhizosphere: control-elevated
Bacterial communities were profiled by amplifying the V4 region of the 16S rRNA gene using primers 515F (5-GTGYCAGCMGCCGCGGTAA-3) and 806R (5-GGACTACNVGGGTWTCTAAT-3) (Apprill, McNally, Parsons & Weber 2015; Parada, Needham & Fuhrman 2016).