GSM9499875: Patient 041,PostNeo, PBMC, RNAseq; Homo sapiens; RNA-Seq
RNA and genomic DNA were extracted from resected tumor specimens preserved in AllProtect tissue reagent (Qiagen) at the time of surgery. We divided the tumor tissue in half and employed the Qiagen RNeasy Mini Kit (#74104) for RNA isolation and the Qiagen DNeasy Blood and Tissue Kit (#69504) for DNA isolation. Total RNA from PBMCs was extracted using the Qiagen RNeasy Mini Kit. TruSeq RNA exome kit is used to construct the RNA sequencing libraries