WES of DXNEN-YZ-006TA
First, genomic DNA samples are subjected to fragmentation treatment, resulting in DNA fragments with sizes mainly ranging from 150 to 200 bp. Subsequently, the fragmented DNA is subjected to end repair to generate blunt-ended fragments with 5' phosphate groups, and Klenow fragment and dATP are used to add adenine to the 3' ends of the DNA fragments to highlight the ends, so as to facilitate subsequent connection of adapters containing index sequences. After adding the A tail, indexing-specific adapters are connected to both ends of the DNA fragments, and AMPure XP magnetic beads are used for purification to remove unconnected adapters and impurities. Next, the library is pre-amplified using SureSelect Primers and Pre-capture Reverse PCR primers to enrich the fragments that have been connected to the adapters, obtaining a DNA library that can be used for hybrid capture.