GSM8720715: E2F1 K117R, SETD6 KO, Replicate 3; Homo sapiens; RNA-Seq
For the DU145 CRISPR/Cas9 generated SETD6 knock-out, single clones were confirmed by sequencing as described in previously (Kublanovsky et al., 2023) total RNA was isolated from approximately 1 × 106 cells by using the RNeasy® Mini Kit (Qiagen). The extracted RNA was quantified by NanoDrop1000 and reverse-transcribed into complementary DNA (cDNA) to generate RNA-seq libraries.