GSM8457692: mEBD5_WT_rep2; Mus musculus; RNA-Seq
Total RNA was extracted using Trizol. Poly-A RNA was captured usuing magnetic oligo-dT beads. RNA-seq libraries were prepared usinig 100ng poly-A enriched RNA after poly-A purification, RNA libraries for RNA-seq were prepared using KAPA RNA Hyperprep Kit following manufacturer's protocols. Quality control on final libraries was assessed by TapeStation fragment analysis prior to pooling. Equimolar amounts of libraries were pooled and sequenced on Illumina HiSeq 4000 and NovaSeq X instruments using standard protocols.