GSM8574891: neonatal mouse organ of Corti, Bmi1 KO, bio rep1; Mus musculus; RNA-Seq
Six mouse organ of Corti (OCs) from WT and Bmi1-/- neonatal mice were separately used.  Each OC was carefully extracted from the cochlea with the anlage of the stria vascularis, the modiolus, and the tectorial membrane removed. Total RNA was extracted with the AllPrep DNA/RNA/Protein Mini kit (QIAGEN). For each genotype we performed three biological replicates. The double-strand cDNA was synthesized from the total RNA LT Sample Prep Kit v2 (Illumina). Illumina adapters were ligated to the cDNA molecules after end repair, and the ligated cDNA was cleaned up with AmpureBeads (Beckman). The library was amplified using 10 cycles of PCR for the enrichment of adapter-ligated fragments, and transcriptome sequencing was carried out with an Illumina-Hiseq2500 system (Illumina).