RNA-Seq of OCM-1A: stable expression of Rv0183 (S110A)
The pcDNA3.1-DV5-His/TOPO vector was used throughout to generate stably expressing cell lines. The Rv0183 coding sequence was amplified from genomic DNA of M. tuberculosis H37Rv. An Rv0183(S110A) mutant was created by site-directed mutagenesis, converting serine-110 to alanine.