16S rRNA sequencing of Homo sapiens: gut microbiome from fecal samples
DNA was extracted from fecal samples using the GENE PREP STAR PI-1200A system. The V1V2 region of the 16S rRNA gene was amplified using primers 16S_27Fmod and 16S_338R. Libraries were prepared with the Nextera XT kit and sequenced on an Illumina MiSeq platform (250 bp paired-end).