Metatranscriptomic RNA-Seq of soil-derived anaerobic enrichment with isoprene: ICE1_A2
RNA integrity and quantity were assessed using a Bioanalyzer 2100 (Agilent Technologies) and a Qubit fluorometer (Thermo Fisher Scientific). Ribosomal RNA depletion and cDNA library construction were performed using standardized Illumina protocols. Libraries were sequenced on an Illumina NovaSeq 6000 platform with paired-end 150 bp reads (PE150), generating an average of ~22 million raw reads per sample, totaling over 88 million reads across all conditions. Sequencing was performed by BMKGENE (Beijing, China)