AAV_sh_PTGES3_1 (CRX1926241; CRA029215)
Two-week-old male C57BL/6 mice were housed under SPF-grade conditions. According to experimental records, the mice were processed in three batches, with each batch randomly and equally allocated to the sh-PTGES3 and sh-NC groups. Liver-specific knockout was achieved in vivo using adenovirus CRISPR/Cas9-sgPTGES3 driven by the hepatocyte-specific ALB promoter, while the control group received non-targeting adenovirus. Intraperitoneal injection of DEN was used to induce orthotopic liver cancer (30 mg/kg weekly at 2-3 weeks of age, 50 mg/kg weekly at 4-9 weeks of age). Body weight and general behavioral performance were monitored weekly. At the 24th week, the mice were euthanized for sample collection, and liver tissues were harvested for subsequent histological and molecular analyses.