GSM8536621: HN_ChIP_144_p300_Nostim_1h_Th2_rep1; Mus musculus; ChIP-Seq
Stimulated or unstimulated cells were fixed for 10 min with 1% formaldehyde (ThermoFisher, #28908). Fixation was quenched by adding glycine (Sigma) at a final concentration of 125 mM. The nuclei preparation, chromatin digestion and chromatin immunoprecipitation were performed using SimpleChIP Plus Enzymatic Chromatin IP Kit (#9005, Cell Signaling Technology) with the antibodies against RNA polymerase II (#ab5408, abcam), p300 (#ab14984, abcam), GATA3 (#5852, Cell signaling), NFAT1 (#5861, Cell Signaling Technology), RelA (#8242, Cell Signaling Technology), CTCF (#07-729, Millipore Sigma) and Rad21 (#ab992, abcam). The immunoprecipitated DNAs were subsequently processed to generate a DNA library using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, # E7645L) and NEBNext® Multiplex Oligos for Illumina (NEB, #E6440S).