GSM9237535: Treg cells from healthy control, TCR-seq, replicate 3; Homo sapiens; OTHER
Tregs (CD4+CD25+CD127dim/-) were isolated from fresh PBMCs using a CD4+CD25+CD127dim/- Regulatory T Cell Isolation Kit for Human (Miltenyi Biotec), in accordance with the manufacturer's instructions. In brief, total CD4+ T cells were isolated by negative selection using an LD column (Miltenyi Biotec). Subsequently, Tregs were purified from the CD4+ T cell population using an MS column (Miltenyi Biotec). The magnetic isolated Treg cells were encapsulated on Chromium Next GEM Chip G (10×Genomics, 1000120) using Single Cell 5' chemistry (v2, PN-1000263), with cellular barcoding performed on a Chromium × controller (10×Genomics). Library preparation using either Chromium Single Cell 5' v2 Reagent Kit (PN-1000263), or Barcoded cells underwent reverse transcription followed by TCR library preparation using 5' Kit and Human TCR V(D)J Enrichment Kit (PN-1000255). All steps followed 10×Genomics User Guide CG000331Rev F.