Chromosome-level assembly of Pilocarpus microphyllus: Hi-C from from a greenhouse-grown leaflet specimen
Total genomic DNA from the leaflets was isolated using the MagAttract HMW DNA Kit (Qiagen). Hi-C library was prepared using the Arima Hi-C 2.0 Kit (Arima), followed by the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs). Hi-C library was sequenced on NextSeq 2000 (Illumina) using a P3 Reagent 300 cycles (2 x 150bp) (Illumina).