GSM8860638: Sample7_DRG_B16F10_rep3; Mus musculus; RNA-Seq
L3–L5 dorsal root ganglia (DRG) were harvested and digested. Most CD45+ cells were depleted using the MagniSort Mouse CD45 Depletion Kit (Invitrogen, #8804-6864). The remaining neurons were resuspended in FACS buffer (PBS with 2% fetal calf serum and EDTA) and stained with Viability Dye eFluor 780 (eBioscience, 65-0865-14) at 4 °C for 15 minutes. Subsequently, nuclei were stained with SYTO 40 (10 µM, Thermofisher, #S11351) for 5 minutes at room temperature to distinguish cells from axonal debris. Cells were then purified by FACS on a BD FACSAria IIu cell sorter Single-cell barcoding was performed using the Chromium Next GEM Single Cell 3′ GEM, Library, and Gene Expression v3.1 system (10x Genomics).