Blank3 (CRX2064000; CRA031318)
Total RNA was extracted from tumor cells using Trizol and quality-checked by Agilent 2100. mRNA was enriched with Oligo(dT) beads, fragmented, and converted to strand-specific cDNA using dUTP for second-strand synthesis. The Illumina sequencing library was completed via end repair, A-tailing, adapter ligation, size selection, PCR amplification, and purification.