GSM8654259: DU145_DMSO rep1; Homo sapiens; RNA-Seq
Total RNA was extracted from the tissue using TRIzol® Reagent according the manufacturer's instructions. Then RNA quality was determined by 5300 Bioanalyser (Agilent) and quantified using the ND-2000 (NanoDrop Technologies). RNA purification, reverse transcription, library construction and sequencing were performed at Shanghai BioMarker Biotechnology Co., Ltd. (Shanghai, China) according to the manufacturer's instructions (Illumina, San Diego, CA)