GSM8966269: OEJMJ4_HA ChIP rep2; Solanum lycopersicum; ChIP-Seq
2g fresh tomato leaves were collected and fixed with 1% formaldehyde. After plant nucleus chromatin isolated, the DNA was sonicated to 100-400bp. 5% DNA was took as input, and the remaining DNA collected for immunoprecipitation. The primary antibody (Abcam, ab9110, Cambridge, UK) was incubated with fragmented chromatin at 4℃ for 6 hours. The antibody magnetic bead protein DNA complex is washed sequentially with high salt and low salt buffer solutions, and then the IP product is eluted. The IP product and input were cross-linked using RNase A (NEB, T3018L, Ipswich MA, USA) and Proteinase K (NEB, P8107S, Ipswich MA, USA). The cross-linked input DNA and IP DNA were purified using a QIAquick PCR Purification Kit (QIAGEN, 28106, Hilden, Germany) and DNA libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645S). The DNA libraries were sequenced on Novaseq PE150 sequencing platform by Romics Biotech (Romics, Shanghai, China).