GSM8574886: NR_7d_Amp.63_L1.07232015.Left; Mus musculus; RNA-Seq
Total RNAs were isolated from tissues using trizol (ThermoFisher #15596018) and chloroform phase separations followed by the RNeasy mini protocol (Qiagen #74106) with optional on-column DNase digestion (Qiagen #79254). One hundred nanograms of total RNA was used to prepare sequencing libraries using the LM-Seq (Ligation Mediated Sequencing) protocol(Hou et al., 2015). RNAs was selected using the NEB Next Poly A+ Isolation Kit (NEB #E7490S/L).