WES of OCI-AML3
Purified genomic DNA is fragmented using a Covaris R230 AFA instrument. Whole genome libraries are prepared using IDT XGen DNAseq reagents on a Beckman Coulter Biomek i7 liquid handling platform. Finished libraries are pooled (typically 8-plex) and exome hybridization capture enrichment is performed using Twist Biosciences probes and reagents. Sequencing is performed with paired-end 150bp reads to specified depth of coverage on an Illumina NovaSeq X Plus.