GSM8758174: GBM1A patient-derived neurospheres, parental; Homo sapiens; RNA-Seq
GBM neurospheres were pelleted and resuspended in 200uL of medium containing EGF/bFGF. The neurospheres were then gently mixed using a wide-bore pipette tip to create a single-cell suspension. The single cell suspension was filtered using a 40um nylon mesh cell strainer to remove cell clumps and debris. Single-cell concentrations were determined using an automated cell counter.  Cells were centrifuged at 500 rcf for 5 min at room temperature and cell pellets were resuspended in PBS with 0.05% BSA according to the recommendations in the Chromium Next GEM Single Cell 5' Reagent Kits v2 protocol to achieve 10,000 cell recovery. Library construction was performed using the 10X Genomics Chromium v2 platform according to standard protocol.