LRa05-3
Single-stranded circular DNA molecules undergo rolling circle replication, forming DNA nanoballs (DNBs) containing multiple copies. The resulting DNBs are loaded into the mesh-like holes on the chip using high-density DNA nanochip technology, and sequencing is performed via combinatorial Probe-Anchor Synthesis (cPAS). Metagenomic sequencing is conducted using the MGISEQ-2000 platform (or DNBSEQ-T7) with PE162 sequencing strategy.