mRNA-seq of Acinetobacter baylyi
RNA extraction and rRNA depletion was performed with the DIY method developed by Culviner et al. The RNA library was prepared with NEBNext Multiplex Oligos for Illumina(NEB) and NEBNext ultra II RNA Library Prep Kit for Illumina(NEB). For the library prep protocol, we followed section 4 of the kit's provided protocol: Protocols for use with Purified mRNA or rRNA Depleted RNA. The quality of the final library was verified by running the samples on high sensitivity Bioanalyzer chip. The samples were pooled to a final concentration of 8.5nM, and were sequenced with NextSeq 150 cycle kit.