RNA-Seq of Piper betle: leaf treated with Abscisic acid
Three replicate samples were collected at each time point. Total RNA was extracted using the Trizol method. RNA integrity was accurately detected by Agilent 2100 bioanalyzer (Agilent technologies, USA), and cDNA libraries were constructed from total RNA that passed the quality control. The Illumina NovaSeq 6000 (Illumina, CA, USA) was used as a sequencing platform, and the raw reads obtained from sequencing were first filtered, and the sequencing error rate and GC content distribution were checked to obtain clean reads for subsequent analysis. Statistical Analysis The treatments were arranged in a completely randomized design (CRD)