metagenomics sequencing of bacterial culture plates from gastric biopsy tissue
Bacterial pellets collected from whole-plate cultures under aerobic, anaerobic, and microaerophilic conditions were pooled per biopsy sample. DNA was extracted from pooled bacterial pellets using the DNeasy PowerLyzer PowerSoil Kit (Qiagen, Germantown, MD) following the manufactures protocol. Metagenomics sequencing libraries were prepared using NexteraFlex and sequenced by 2250 bp paired reads on an Illumina Novaseq 6000 instrument.