GSM8510468: female_vehicle_318; Mus musculus; RNA-Seq
After infusion of magnetic beads via renal artery, kidney cortex was digested and podocytes were isolated utilizing magnetic bead separation. Separation of GFP positive podocytes from single cell fraction was carried out via fluorescence-activated cell sorting (FACS). Total RNA was harvested with Trizol protocol. NEB Next Ultra II Directional RNA Library Prep Kit was used following manufactures instructions