Oxford Nanopore Seq of S. pombe/S. pombe var. kambucha hybrid
S. pombe/S. pombe var. kambucha hybrid diploids were assayed for loss of heterozygosity (LOH). LOH control and 10 candidate colonies were isolated and cultured in minimal media + supplements. High molecular weight genomic DNA was extracted using NEB HMW gDNA extraction kit (#T3060L). Library preparation was conducted using Oxford Nanopore Native Barcoding Kit 24 V14 (SQK-NBD114.24). Samples were run on a MinION Mk1C and reads were basecalled using Oxford Nanopore's basecaller Dorado.