GSM8828412: DLDPh_aux_1; Homo sapiens; RNA-Seq
RNA was extracted with Qiagen RNAeasy Plus Mini Kit. Extracted RNA was analyzed for 260/280 and RIN value was assessed on Bioanalyzer 2100 (Agilent), samples with RIN value more than 9 were proceeded for the library construction using Illumina TruSeq Stranded Total RNA Library Preparation Kit (Illumina) according to manufacturer's instructions. 1-4 ug of total RNA samples  were depleted for ribosomal RNA (Set 2021) before library construction using Ribo-Zero™ Gold Kit H/M/R Kit (Illumina) or purified with PolyA extraction (Set 2024), and then purified mRNAs were constructed to RNA-Seq libraries with specific barcodes using illumina TruSeq Stranded mRNA Library Prep Kit. All the RNA-Seq libraries were pooled together and sequenced using illumina NovaSeq 6000 to generate approximately ~40 million 2x100 paired-end reads for each sample.