DNAseq of MCC tumors
DNA that passed our quality control standards was submitted to the University of Michigan Advanced Genomics Core for targeted capture sequencing using the DNA ThruPLEX kit (Takara Biosciences). Using a custom designed probe panel from Nextera, targeted capture was performed with high density probes covering the MCPyV genome and all exons of 226 known MCC-related genes, targetable genes, and cancer-related genes. Following library preparation and capture, samples were sequenced on an Illumina HiSEQ4000 with paired end 150nt reads.