GSM8966510: LNCaP, R1881, pGIPZ, H3K4me1 ChIP; Homo sapiens; ChIP-Seq
PCa cells were crosslinked with 1% formaldehyde for 10 min at room temperature with gentle rotation and then quenched for 5 min with 0.125 M glycine. Chromatin was sonicated to an average length of 200–600 bp using an E220 focused-ultrasonicator (Covaris). ChIP–seq libraries were prepared from 3–5 ng of ChIPed DNA using NEBNext Ultra II DNA Library Prep Kit, according to the manufacturer's instructions.