LdoS J-IP
Phenol-extracted genomic DNA was fragmented to 200 bp using a Covaris, and fragments containing base J immunoprecipitated by incubation with anti-J antibody (PMC3684241) and Protein A Dynabeads. After elution of the J-containing fragments, they were end-repaired and ligated with PCR adapters before purification using AMPure XP SPRI beads. Ligated DNA was amplified using KAPA HiFi HotStart for 12 cycles and purified again using the AMPure beads.