16S gene amplicon sequencing of DEP degrading microbial consortium
DNA was extracted from using the Macherey Nagel Nucleospin Microbial DNA kit. Extracted DNA was sent to BGI for sequencing. The V3-V4 hypervariable region was amplified using the 338F (ACTCCTACGGGAGGCAGCAG) and 806R (GGACTACHVGGGTWTCTAAT) primers. Sequencing on the DNBSEQ-G400 platform in the paired-end mode with a read length of 300 bp per read.