Mouse gut bacterial 16S rRNA gene sequencing
Total genomic DNA was extracted from mouse cecal contents using fecal DNA extraction kit. The V4 region of fulllength 16S rRNA gene was amplified by PCR with forward primer AGRGTTTGATYNTGGCTCAG and reverse primer TASGGHTACCTTGTTASGACTT. Amplified products were qualitychecked by agarose gel electrophoresis, purified and recovered to construct sequencing libraries. Raw sequencing data were qualitycontrolled and assembled, followed by diversity analysis, PCoA and taxonomic annotation to assess gut microbiota diversity and composition.