GSM8827172: Rpp21-rep1; Mus musculus; OTHER
After partial RNase I digestion of cell lysates, immunoprecipitation was performed using anti-Flag or anti-PABPC1 antibodies, followed by 3' adapter ligation, SDS PAGE, and transfer of protein-RNA complexes to a nitrocellulose membrane from which RNA was extracted in the presence of proteinase K. iCLIP libraries were constructed following the protocol described in Buchbender et al (Methods, 2020; PMID: 31610236)