Genomic Immunoglobulin Heavy and Light Chain region Sequencing from NF14 using PBMCs
Genomic DNA from PBMCs was sheared, size-selected, and enriched for immunoglobulin heavy and light chain loci using oligo probes. Barcoded libraries were PCR-amplified, SMRTbell-prepared, and sequenced on the PacBio platform to generate HiFi reads