GSM8759547: NK92_PD-1_PPI_BioRep2; Homo sapiens; RNA-Seq
Cell pellets containing 1 to 2 million cells were lysed with Buffer RLT Lysis buffer (Qiagen, 1015762, 350 µL) then stored at -80⁰C. RNA was prepped with the RNEasy mini Qiagen kit. The RNA samples were submitted for paired-end RNA sequencing by GENEWIZ, and they were sequenced on the Illumina Nextseq to a depth of ~20 million reads per sample.