GSM9022667: WT replica 3; Mus musculus; RNA-Seq
To isolate total E13.5 fetal liver cells, livers from E13.5 embryos were harvested, and single-cell suspensions were prepared by gentle pipetting in cold 1× PBS supplemented with 10% FBS. Dead cells were removed prior to library preparation. Expression libraries were constructed according to the Chromium 10X single cell 5′ reagent protocol.