GSM9139937: PFC DO Low Performer #2; Mus musculus; RNA-Seq
Mice were sacrificed by cervical dislocation and immediately decapitated. The targeted brain regions were harvested from 1 mm brain slices, obtained by brain matrices (ZIVIC) using 1.0 mm tissue punches and transferred to a tube containing 300 µL of ice-cold lysis buffer and 3 µL ß-mercaptoethanol (Total RNA Purification kit, NORGEN; following the manufacturer's protocol). Samples were then homogenized by passing a 25G insulin syringe six times and left on ice. For RNA extraction, the total RNA Purification kit was used according to the manufacturer's instructions (NORGEN). RNA libraries were prepared from 100ng of total RNA per sample using the TruSeq stranded mRNA LT kit (Cat# RS-122-2101, Illumina).