Sequencing of rumen microbiota in dairy calves
According to the protocols of manufacture, DNA from rumen fluid and fecal samples was extracted using the MagAtrract PowerSoil Pro DNA Kit (Qiagen, Hilden, Germany). The bacterial 16S rRNA gene was amplified using universal bacterial primers 27F and 1500R. All amplicons were sequenced following the standard protocols on the PacBio Sequel IIe System (Pacific Biosciences, CA, USA).