RNA-Seq of homo sapiens: adult male hypertrophic scar
Total RNA was extracted using trizol method. All the RNA samples were assessed for their integrity using Qsep400 instrument. To construct RNA libraries with the VAHTS mRNA-seq V8 Library Prep Kit for Illumina, 1 g of total RNA was used. The procedure included polyA-selected RNA extraction, RNA fragmentation, random hexamer primed reverse transcription, and 150nt paired-end sequencing by Illumina Novaseq 6000.