RNAseq of GBM tumors with SAT1 knockout
RNAseq of GBM tumors with SAT1 knockout
10mg of tumor tissue was harvested from the brains of tumor-bearing mice Tissue was homogenized in 1ml TRIzol (Invitrogen, Cat# 15596018) and homogenized using a BeadBug tissue homogenizer (Benchmark Scientific, SKU: D1030) and porcelain beads for 3 x 10 second pulses.  poly A purification, cDNA preparation were conducted by Novogene