GSM8967019: WT_GEX; Mus musculus; RNA-Seq
Lamina propria cells were isolated from small intestines of two mice of each genotype. Isolated cells were negatively selected for T cells and ILCs (STEMCELL Technologies 19851) and stained with TotalSeq-C anti-mouse hash-tagged antibodies (BioLegend Totalseq-C; Hashtags 7 and 8 for WT; Hashtags 9 and 10 for A20ZF7 ). Cells from each animal were separately hashtagged. WT cells hashtagged with barcodes GAGTCTGCCAGTATC and TATAGAACGCCAGGC A20ZF7 cells hashtagged with barcodes TGCCTATGAAACAAG and CCGATTGTAACAGAC For each genotype, a total of 60,000 cells were collected for processing using the 10X Genomics Chromium Next GEM Single Cell 5' Kit v1.1 per manufacturer's protocol.