GSM9679558: Day 6 wound, C57BL/6 mouse, 2 months old; Mus musculus; RNA-Seq
Total RNA was extracted from single-cell suspensions prepared from day 6 dorsal wound tissue. Single cells were encapsulated using the 10x Genomics Chromium Controller. Reverse transcription and cDNA amplification were performed per manufacturer instructions (10x Genomics Chromium Next GEM Single Cell 3' Reagent Kit v3.1). Gene expression libraries were prepared using the 10x Genomics Chromium Next GEM Single Cell 3' Reagent Kit v3.1 following manufacturer's protocol. Library quality was assessed using an Agilent Bioanalyzer High Sensitivity DNA chip and quantified by qPCR prior to sequencing on an Illumina NovaSeq 6000 (paired-end, 28x91 bp).