GSM8967387: Hezuo pig1, liver, Day 15, -15℃±2℃; Sus scrofa; RNA-Seq
Extract total RNA from liver tissues of all pigs in the control group and the cold treatment group for 15 days using TRIzol (Life Technologies, Carlsbad, CA, USA) First, ribosomal RNAs (rRNAs) are removed, while mRNA and ncRNA are retained. The RNA is randomly broken into small fragments by high temperature. Then, using them as templates, the first strand of cDNA is reverse transcribed with random primers, and the second strand of cDNA is synthesized. Next, purify the cDNA fragment, perform terminal repair, add poly (A), and attach it to the Illumina sequencing adapter. Then, UNG (Uracil-N-Glycosylase) was used to digest the second-strand cDNA. The digested products were subjected to agarose gel electrophoresis to select appropriate fragments, PCR amplification was performed, the library was establis