Long read sequencing of western chicken turtle
High-molecular-weight DNA was sheared to ~13 kb using a Megaruptor 3 and prepared with the SMRTbell Express Template Prep Kit 2.0. The library was quality-checked with Qubit and Femto Pulse and sequenced on three SMRT Cells 8M using the PacBio Sequel IIe with a 30 hs movie time. Reads were processed in real-time with SMRT Link v11.1, using default parameters for circular consensus sequencing.