GSM8547483: HCT116 WT, BRCA2; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a sonicator (Qsonica). ChIP-seq libraries were prepared using the VAHTS Universal DNA Library Prep Kit for Illumina V3 from Vazymes. 1 ng of DNA was used as starting material for input and ip samples. Libraries were amplified using 13 cycles on the thermocycler. Post amplification libraries were size selected at 250-450bp in length using AMPure XP beads.