RNA Seq of lateengorged Female Ixodes ricinus salivary gland
We extracted total RNA from the salivary glands of pools late engorged adult female I. ricinus using the Qiagen RNeasy Mini Kit along with  in-column DNase treatment with Qiagen DNase. We then created cDNA libraries using 100 ng of the extracted total RNA with the NuGEN Ovation Universal RNA-Seq System. This process included removing ribosomal RNA (rRNA) with custom probes made for tick rRNA.