GSM9313117: Mouse ESC_WAPL/CTCF-AID indARID3A Clone 1, IAA 48h; Mus musculus; RNA-Seq
Cells were collected and isolation of total RNA was performed by using the Isolate II RNA Mini Kit (Bioline). RNAseq libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, NEB) and NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB) according to the manufacturer's protocol. RNA quality and yield were measured using RNA integrity number (RIN) algorithm, using the Fragment Analyzer (Agilent) or LabChip Gx Touch 24 (Perkin Elmer). Samples with 7.0-10.0 RIN value were used for library preparation. 2 μg of total RNA was used as input for VAHTS Stranded mRNAseq Library preparation. Sequencing was performed on an Illumina NextSeq550 (75bp pair-end reads) or or an Illumina NextSeq2000 (60bp pair-end reads).