GSM9211114: NC1; Mus musculus; RNA-Seq
CT26 tumors were enzymatically dissociated into single-cell suspensions, and the cell concentration was adjusted to 600–1200 cells/μL. Library preparation was performed using the DNBelab C4 scRNA-seq kit (MGI) with droplet-based mRNA capture. The workflow included cDNA synthesis, amplification via PCR and Fragmentase treatment, end repair, A-tailing, and adapter ligation. Subsequently, the libraries were loaded onto DNB nanoarrays and sequenced using Combinatorial Probe-Anchor Synthesis (cPAS) on the DNBSEQ-T7 platform.