Long reads metagenomics chicken caecal soyabean meal 26
Genomic DNA was extracted from chicken caecal samples (approximately 200mg digesta per sample) using the ZymoBIOMICS DNA Miniprep Kit (Zymo Research, Cambridge, UK). The extracted gDNA (200ng per sample) was sequenced for 72h using ONTs Rapid Barcoding (SQK-RBK114.24) protocol, real-time MinION platform (Oxford Nanopore Technologies, Oxford, UK).