GSM9643350: Brain immune cells from aged mice, CA, Day 3; Mus musculus; RNA-Seq
Mice were intubated, anesthetized with isoflurane, and transcardially perfused. Brains were immediately harvested and dissociated mechanically. Immune cells were enriched using Percoll. Cell suspensions were resuspended in FACS buffer and subjected to FACS sorting to collect CD45⁺ cells. The sorted cells were then processed for library preparation and sequencing. 10x Genomics CG000315 Chromium NextGEM Single Cell 3' Gene Expression v3.1, Dual Index, Rev. E