fusheA_treat-1 (CRX2059677; CRA031180)
mRNA with polyA tails was enriched using Oligo(dT) magnetic beads. Subsequently, fragmentation buffer was added to break the RNA into short fragments. Using these short-fragment RNAs as templates, the first-strand cDNA was synthesized with random hexamer primers. Then, buffer, dNTPs (dTTP, dATP, dGTP, and dCTP), and DNA polymerase were added to synthesize the second-strand cDNA. The double-stranded cDNA was then purified using DNA purification magnetic beads. The purified double-stranded cDNA underwent end repair, A-tailing, and sequencing adapter ligation. Fragment size selection was performed using DNA purification magnetic beads, and finally, PCR enrichment was carried out to obtain the final cDNA library. submitted_instrument_model: Illumina NovaSeq 5000