GSM9021554: HBMVEC, Control, 5, 72h; Homo sapiens; RNA-Seq
After treatment, cells were washed with PBS and total RNA was extracted using TRIzol reagent according to the manufacturer's instructions. RNA concentration and integrity were assessed using Quant-IT RiboGreen and TapeStation RNA ScreenTape. Only samples with RIN > 7.0 were used. 0.5 μg of total RNA per sample was used to construct libraries with the TruSeq Stranded Total RNA Library Prep Gold Kit (Illumina). rRNA was removed using the Ribo-Zero kit, mRNA was fragmented, and cDNA was synthesized with SuperScript II and random primers. Libraries were PCR-enriched, quantified, and quality-checked before sequencing.