GSM9351493: HEK_PRE_ADARcdMUT-1000ng_rep3 (RBPscan); Homo sapiens; OTHER
Total RNA from zebrafish embryos was extracted using TRIzol (ThermoFisher #15596026). Sequencing was performed on PCR amplicons of the 3' UTR of Recorder mRNA containing 1,000 PRE sequences. The PCR reaction was carried out to amplify the 3' UTR region of the Recorder mRNA, which includes PRE motifs and the Recorder hairpin. Partial Illumina adapters present in the Recorder mRNA are complementary to sections of the i5 and i7 TruSeq Illumina primers, which also contain unique barcodes and sequencing adapters.