GSM8458228: WT_sWAT_biol rep 3 (forward strand); Mus musculus; RNA-Seq
Total RNA (RIN value > 7) was extracted from adipose tissues, and poly-A pull-down was performed to enrich mRNAs. Library construction was performed using Illumina TruSeq chemistry, with the final PCR step being modified using the KAPA HiFi HotStart Ready Mix.