GSM8927372: p22286_s088_L990_Male_20_21_Group_3_D0_Post_ATI_BatchA; Macaca mulatta; RNA-Seq
Total RNA was extracted from whole blood samples using the PAXgene Blood RNA Kit IVD (PreAnalytix). RNA quality was assessed with the Fragment Analyzer (Agilent) and its Standard Sensitivity RNA kit. Total RNA was normalized to 100 ng before random hexamer priming and libraries were generated by the TruSeq Stranded Total RNA—Globin kit (Illumina). The resulting libraries were assessed on the Fragment Analyzer (Agilent) with the High Sense Large Fragment kit and quantified using a Qubit 3.0 fluorometer (Life Technologies). Medium-depth sequencing, with at least 30 million reads per sample, was performed on a NextSeq 500 (Illumina) with a PE100 configuration, paired-end run yielding ~40 million reads per sample.