RNA-Seq of T. brucei: A6 from BF KREPA6 CN MGA plus tet
Total RNA was extracted from T. brucei and reverse-transcribed using gene-specific primers for either MURF2 or A6 amplicons. cDNA was amplified and sequenced using 300 by 300 paired end sequencing. Unique molecular identifiers (UMIs) were included at both the 3' and 5' ends of each amplicon.