RNASeq of Mus musculus: M0 BMDM_Mouse 2
BMDMs were isolated and differentiated as above and then either treated for 5 days with 50ng/mL Wnt3a or cultured in control 20% L929CM. Media was changed and fresh Wnt3a added every other day, for a total of 3 treatments in 5 days. On the 5th day, RNA was isolated as above. RNA quality control analysis was performed with Agilent TapeStation, and all samples were determined to have RNA integrity number equivalent (RINe) of 10/10 (highest quality). mRNA enrichment and cDNA library preparation was performed utilizing the the NEBNext Poly(A) selection kit, and library quality control analysis was performed using Qubit and the Agilent BioAnalyzer. Sequencing was performed at Paired-End 150 bp on the Illumina NovaSeq 6000 targeting an average of 50M reads per sample.