WGS of Savannah F 5th instar larva
Genomic DNA was extracted from three 5th instar Savannah female larvae and three 5th instar bFog female larvae using the Quick-DNA HMW DNA MagBead Kit (Zymo Research) following the manufacturers protocol. DNA was quantified using Nanodrop and checked with 1% agarose gel. gDNA was sheared with g-TUBE (Covaris) for PacBio library construction. PacBio libraries were generated using SMRTbell Prep Kit 3.0 with barcoded adapters (PacBio) according to the manufacturers protocol and library QC was performed using Qubit and TapeStation.