GSM9682491: GBe8s7_cordBlood-CG_BRG1-2; Homo sapiens; OTHER
Transduced cord blood cells were harvested, washed, and processed for CUT&Tag as described for cell lines. Nuclei were incubated with antibodies targeting SMARCA4 (BRG1), H3K27ac, H3K4me3, or IgG control, followed by secondary antibody and pA-Tn5 incubation. Tagmentation was activated by MgCl₂ at 37°C and resulting DNA fragments were purified. CUT&Tag libraries were prepared in-house using the CUT&Tag protocol (Kaya-Okur et al., 2019) and sequenced in-house at the Seattle Children's Research Institute Genomics Core. Paired-end sequencing was performed on an Illumina instrument.