GSM8859983: Hela cells-K700E_rep3; Homo sapiens; ncRNA-Seq
Hela and HEK293T cells with or without SF3B1-K700E mutation was harvested using Trizol reagent.Total RNA was extracted using the RNeasy Mini Kit (Cat#74106, Qiagen).Total RNA was depleted ribosomal RNA and then useds RNase R to digest linearRNA.The RNA was fragmented and synthesized cDNA with random primer. Purified the PCR amplification products of cDNA, then the libraries were quality controlled and sequenced. RNA libraries were prepared for sequencing using standard Illumina protocols