GSM9350095: iPSC_VSMC_Het_rep_2; Homo sapiens; RNA-Seq
The cell pellets were dissociated using TRIzol (15596018 Invitrogen). RNA was then extracted using a Zymo Direct-zol RNA Miniprep kit (R2052) according to the manufacturer's protocol. 300ng total RNA was converted to single stranded cDNA and amplified using the Iso-Seq Express 2.0 Kit (Pacific Biosciences). Iso-Seq libraries were constructed with the SMRTbell Template Prep Kit 3.0 (Pacific Biosciences).