16S targeted metagenome of pig feces
The Genomic DNA of samples were extracted using Qiagen Mini Stool kit DNA Isolation Kit following the manufacturers guidelines to identify the microbial community of the mouse feces. Amplicons from individual  mouse feces gDNA samples were amplified using primers for the V3-V4 hypervariable regions of the 16S rRNA gene by polymerase chain reaction (PCR). For each sample, Barcoded V3-V4 PCR amplicons were sequenced by Illumina Miseq platform.