GSM9429400: SAT, miRNAs, rep5; Oncorhynchus mykiss; miRNA-Seq
Adipose tissue was dissected from fish (visceral, subcutaneous, and intramuscular depots). Mature adipocytes were isolated by mechanical and enzymatic digestion using collagenase type II. Total RNA, including small RNA species, was extracted using TRIzol reagent (Thermo Fisher Scientific, Waltham, MA, USA), followed by chloroform, isopropanol, and ethanol extraction according to the manufacturer's instructions. RNA quality and integrity were assessed prior to sequencing. Small RNA libraries were prepared by Azenta/GENEWIZ (Leipzig, Germany) from total RNA following standard Illumina-compatible small RNA library preparation protocols. Libraries were sequenced on an Illumina platform. Sequencing quality control ensured that at least 80% of bases had Q-scores ≥ Q30.