RNA-Seq of HUVECs isolated from umbilical cord treated with DOX
Total RNA was extracted from HUVECs treated with DOX utilizing TRIzol reagent, subsequently undergoing quality assessment via the 1% RNA Nano 6000 Assay Kit and the NanoPhotometer spectrophotometer (IMPLEN, CA, USA) to ascertain both RNA integrity and purity. Sequencing libraries were constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA), adhering meticulously to the manufacturers protocol. mRNA was isolated from total RNA with poly-T oligo-attached magnetic beads. Library preparation and quality assessment were conducted on the Agilent Bioanalyzer 2100 system. These library preparations were then sequenced on an Illumina NovaSeq platform. Differential expression analysis was carried out employing the DESeq2 R package (version 1.16.11)