M6
Genomic DNA was extracted from frozen cecal samples using a guanidinium thiocyanate-based method with bead beating. DNA was purified using the Magtration System 12GC and MagDEA DNA 200 kit (GC series). The V3V4 region of the 16S rRNA gene was amplified using Pro341F and Pro805R primers. Sequencing was performed on the Illumina MiSeq platform (2  300 bp).