GSM8508874: prp43a mutant, heat stress, biological replicate #2; Arabidopsis thaliana; RNA-Seq
Total RNA was extracted from Arabidopsis seedlings using Quick-RNA Plant Miniprep Kit (Zymo Research). RNA-seq libraries were constructed using TruSeq Stranded mRNA library preparation kit (Illumina) and paired-end sequencing was performed to produce 2 X 75-bp reads using the Illumina Miseq platform.