GSM9464519: SAP_rep 2; Rattus norvegicus; OTHER
For spatial transcriptomics, ileal tissues were embedded in OCT within 30 min after resection, snap-frozen on dry ice, stored at −80°C, sectioned at 10 μm on a cryostat, and mounted onto Stereo-seq chips for downstream permeabilization and reverse transcription. For Stereo-seq, libraries were generated following the STOmics Gene Expression Set-S1 protocol. After tissue staining and imaging, cDNA was released from the chip following permeabilization (6–24 min optimized per sample), reverse transcription was performed, and indexed libraries were constructed per the manufacturer's instructions.