GSM8966321: Ls889 Red Parts Rep 4; Lactuca sativa; RNA-Seq
RNA was extracted used TRIzol A 3' sequencing adapter was ligated, followed by reverse transcription using a template switching RT. Barcodes were then ligated to the ends of the cDNA using NEBNext DNA library kit and pooled together. The pooled libraries were then subject to target capture enrichment using biotinylated probes, followed by ligation of ONT sequencing adapters and sequencing