sequences of fishes
The concentration of DNA was detected using Qubit, and the PCR amplification primers, the reported universal primers for fish MiFish, were selected to amplify the 12S rRNA region of the sample(Miya et al., 2015). A negative control was established using distilled water to exclude contamination during the experiment. Finally, the library was subjected to paired-end sequencing on the Illumina NovaSeq platform (commissioned by Yijian Biotechnology (Jiangsu) Co., Ltd.)