GSM8966356: liver, young, SIRT6 tg, rep7; Mus musculus; OTHER
High molecular weight genomic DNA was extracted from liver tissue using the Monarch® HMW DNA Extraction Kit, involving tissue homogenization, proteinase K digestion, RNase A treatment, phase separation, NaCl precipitation, and isopropanol-based DNA retrieval using glass beads. The purified DNA was eluted, and concentration was measured via Nanodrop. DNA was sheared using a Covaris g-TUBE, size-checked on TapeStation and Qubit, and processed using the Ligation Sequencing Kit V14 with adapter ligation and cleanup. Sequencing was carried out on the PromethION 24 platform using R10.4.1 flow cells, with each sample run for 72–112 hours, producing high-coverage long-read data