RNA-seq of Chrysomya megacephala: whole adult female
The sample used for sequencing contained 1-2g of total RNA. The library preparation and sequencing was outsourced to the BGI Genomics sequencing center (Tai Po, Hong Kong). To prevent sample degradation during transportation, RNA was precipitated by the addition of absolute ethanol (2.5x sample volume) and 10% 3M sodium acetate. The TruSeq mRNA protocol (Illumina, San Diego, USA) was used to prepare the libraries. They were sequenced using HiSeq 2500 equipment using the HiSeq SBS v4 kit (Illumina, San Diego, USA), with paired 100 bp or 150 bp reads.