H3K27ac ChIP-Seq from Primate iPSC derived Astrocytes (iAstrocytes)
H3K27ac ChIP-Seq from Primate iPSC derived Astrocytes (iAstrocytes)
Cells were detached with Accutase, counted and crosslinked with 1% (final) formaldehyde in their medium. Reaction was quenched with 0.125M (final) glycine, cell pellets were washed with PBS, snap frozen and stored in -80 freezer. Pellet of 3 million cells was used for H3K27A ChIP experiment. iAstrocytes were grown on hESc-qualified Matrigel (Corning)-coated plates, in Astrocyte Medium (ScienCell). Seeding density was 15,000 cells/sq. cm. ChIP prodecure was performed as described before (Pekowska et al., 2018, Cell). Libraries were constructed using Ovation Ultra System V2 (Tecan, 0344NB-32), according to manufacturer's instructions.