SFBKRNA
Cervical Cancer Blood samples were collected from n=10 cervical cancer patients (Stage I to Stage IV) and 20 age-matched healthy controls. Total RNA was extracted using TRIzol LS Reagent (Invitrogen) following the manufacturers protocol, RNA, integrity (RIN > 8.0) was confirmed using the Agilent 2100 Bioanalyzer. Equal volumes of total RNA from individual samples were pooled before library preparation to reduce sequencing costs, cDNA libraries prepared using the Illumina TruSeq kit. RNA Sequencing was performed using the Illumina Hiseq 150 bp paired-end sequencing to generate 25-30 million reads (R1+R2). The RNA sequencing Quality Control was done with FastQC (v0.11.2), Trimming with TrimGalore (0.6.7), Alignment with STAR (2.6.1d), Sorting with Sort SAM and Marked the duplicates, Quantification with Salmon (1.10.1) and the differential expression analysis with DESeq2 (1.50.2).