GSM8453138: ZAP-HA in MT4, rep1; Homo sapiens; RIP-Seq
ZAP:RNA complexes were crossedlinked by exposure to UV radiation for 1min. Cells were lysed and ZAP was purified by immunoprecipitation. RNA bound to ZAP was released by proteinase K digestion and phenol:chloroform:isoamyl alcohol extraction. A 3' adapter was ligated to the RNA, gel purified and subsequently ligated to a 5'adapter. Resulting nucleic acids were reversed transcribed and amplified using Illumina primers.