GSM8457924: CD8+ T cells from healthy individuals, irradiated (2Gy), 24h, rep2; Homo sapiens; RNA-Seq
Total RNA was extracted using RNAiso Plus (Takara). The extracted RNA was assessed for quality by measuring the absorbance ranging from 1.8 to 2.1 using a NanoDrop 2000 Spectrophotometer (ThermoFisher), and RNA integrity was assessed by Bioanalyzer 2100 (Agilent). RNA-seq libraries were prepared using the NEBNext Ultra RNA Library Prep Kit from Illumina following the manufacturer's instructions (New England Biolabs).