RNA-seq of aerobic granule
Bacterial ribosomal RNA (rRNA) was removed using QiaSeqFastSelect 5S/16S/23S kit (Qiagen) following the manufacturer recommendations on 1 g of total RNA. Libraries were then generated using the Truseq Stranded mRNAribo zero sample preparation kit (Illumina, USA). The sequencing analysis was performed at the Lausanne Genomic Technologies Facility, University of Lausanne (Switzerland) on NovaSeq 6000 in paired-end mode (2x150).