GSM8828009: HSJD-DIPG-007 Day 1 24 Gy; Homo sapiens; RNA-Seq
Cells were stored in RLT with beta mercaptoethanol snap frozen at -80. Gentle thawing, and RNA extraction was carried out as per instructions, RNA was extracted from both non-irradiated (proliferative) and irradiated (senescent) human DMG cells using the Rneasy Micro kit (Qiagen). Total RNA integrity was validated using Agilent's 4200 Tapestation, with all samples having RIN values >9.4. UCL genomics core facility prepared cDNA libraries using 250ng of total RNA and the KAPA mRNA HyperPrep Kit. High-quality libraries were confirmed on the Agilent TapeStation 4200. Samples were pooled, normalized to 4nM using the Normalase assay, and sequenced on the NextSeq 500 instrument with a 75bp paired-end run.