GSM9302172: pituitary, HFD_1; Mus musculus; RNA-Seq
Pituitary single-cell RNA sequencing (scRNA-seq) was performed with three mouse pituitary tissues per group collected as one sample. The pituitaries were placed in tissue preservation solution (130-100-008, Miltenyi, Germany) and sent to LC Bio Technology Co., Ltd. (Hangzhou, China) for sequencing and analysis. Briefly, the collected pituitaries were minced and incubated with pre-prepared enzyme solution (130-107-677, Miltenyi, Germany) at 37℃ for 1 h. Cell counting was conducted using a Countess™ II Automated Cell Counter (ThermoFisher, USA). Cell viability was determined by trypan blue staining, and the viability rate of viable cells was above 90% in all samples. Single-cell 3' cDNA libraries were generated using the 10× Genomics platform (USA) according to the manufacturer's protocol. RNA sequencing was then performed on the Illumina NovaseqTM 6000 platform (LC Bio Technology, Hangzhou, China).