GSM9352154: Pooled colon tumor cells from control Hmox1 fl/fl mouse A; Mus musculus; RNA-Seq
Colonic tumors were pooled per mouse, minced,and cells enzymatically dissociated with 3 mg/mL collagenase type IV and Dnase I 75 U/mL in complete RPMI media for 30 minutes at 37 degrees celcius. Live cells were then stained with a viability dye (7AAD) and sorted uing a FACSAria. Library construction was performed according to the manufacturer's instructions (Next GEM 3' Gene Expression v3, 10X Genomics)