Standard NextSeq 500 paired-end Sequencing; RNA-seq upon optogenetic depletion of the cofactor Chip in Drosophila melanogaster embryos at 2-3h, 2-4h, 6-7h and 10-12h after egg-laying
RNA-seq upon optogenetic depletion of the cofactor Chip in Drosophila melanogaster embryos at 2-3h, 2-4h, 6-7h and 10-12h after egg-laying
All fly lines were maintained in a standard cornmeal-agar medium and grown at 25°C. ; mRNA-seq Libraries were prepared from 1 μg of RNA using the NEB Ultra Directional RNA Library Prep Kit for Illumina (NEB N.E7420S), following the Protocol for use with NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB N.E7490).