Illumina MiSeq paired end sequencing
RNA from sorted γδ T cells was reverse-transcribed using template-switch anchored RT (SuperScript II) primed with TRDC outer primers, followed by nested PCR amplification of TRD using TRDC inner primers plus a template-switch adaptor primer. Amplicons were bead-purified (AMPure XP), indexed (Nextera XT), and sequenced paired-end on an Illumina MiSeq.