DNA-seq of Drosophila melanogaster: whole adult male
NEBNext Ultra DNA Library Prep Kit for Illumina, clustering, and sequencing reagents was used throughout the process following the manufacturers recommendations. Briefly, the genomic DNA was fragmented by acoustic shearing with a Covaris S220 instrument.  Fragmented DNA was cleaned up and end repaired. Adapters were ligated after adenylation of the 3ends followed by enrichment by limited cycle PCR. DNA libraries were validated using a High Sensitivity D1000 ScreenTape on the Agilent TapeStation (Agilent Technologies, Palo Alto, CA, USA), and were quantified using Qubit 3.0 Fluorometer.   The DNA libraries were also quantified by real time PCR (Applied Biosystems, Carlsbad, CA, USA).