GSM8861085: GE IgG ChIPseq rep 3; Mus musculus; ChIP-Seq
For CUT&RUN, nuclei were extracted from flash frozen ganglionic eminence harvested from E13.5 wildtype mice, 1 million nuclei were used per sample. For ChIPseq, ganglionic eminences from E13.5 wildtype mice were harvested, dissociated into single cells and fixed in 1% PFA for 30 minutes. The fixed cells were lysed and chromatin were sheared by sonication. CUT&RUN ibraries were constructed using Epicypher CUTANA CUT&RUN Library preperation kit according to manufacterer instructions. ChIPseq libraries were constructed using Illumina Nextera library preparation kits, performed according to manufacterer instructions.