RNA seq of Malus domestica leaf control
For library preparation, 1 g of total RNA was used to construct cDNA libraries with the TrueSeq Stranded mRNA Library Preparation Kit (Illumina, San Diego, CA). This method selectively enriches polyadenylated mRNA using oligo(dT) beads, followed by fragmentation, first and second-strand synthesis, A-tailing, adapter ligation, and PCR amplification to generate strand-specific libraries.