RNA-seq of wild-type M. tb (log)
RNA was extracted using the Monarch Total RNA Miniprep Kit (NEB) and treated with the NEBNext rRNA Depletion Kit for Bacteria (NEB). Library was then constructed with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina, according to the manufacturers protocol. The cDNA library was sequenced using a 2x150 mode.