GSM8459644: Asxl1, replicate 1, scRNAseq; Mus musculus; RNA-Seq
P14 cells were FACS-purified from spleen at 30 days post LCMV infection. Purified P14 cells were washed with PBS and 0.04% BSA before loading into a Chromium Next GEM Chip G (10X Genomics). GEM generation, cDNA amplification and library construction were performed following the protocol of Chromium Next GEM Single Cell 3′ Reagent Kits v3.1 (Dual Index, 10X Genomics) with target recovery of 5000-10000 cells per library/sample. The final 4 libraries were sequenced on the Illumina NovaSeq platform using 100bp paired-end with target reads of 500 million per sample. GEM generation, cDNA amplification and library construction were performed following the protocol of Chromium Next GEM Single Cell 3′ Reagent Kits v3.1 (Dual Index, 10X Genomics) with target recovery of 5000-10000 cells per library/sample