ATAC-Seq of hordeum vulgare: leaf
nuclei were processed using Tagment DNA Enzyme (TDA1, Illumina, 20034197), purified using the MinElute PCR Purification Kit (QIAGEN, 28004), amplified using the NEBNext High-Fidelity 2x PCR Master Mix (NEB, M0541), and further purified using the VAHTSTM DNA Clean Beads (Vazyme, N411)