GSM8860493: Small intestine, day 2, rep2; Mus musculus; RNA-Seq
Whole duodenal tissue was harvested and flash frozen and stored at −80 °C. Total RNA was extracted by crushing the tissue using a pestle and mortar on dry ice and extracted by using the QIAGEN RNeasy Mini Kit as per the manufacturer's instructions. In all, 1 μg of RNA was reverse-transcribed by QuantiTect Reverse Transcription Kit (Thermofisher). Extracted RNA was controlled for integrity using the Agilent Bioanalyzer (RNA Analysis), Stranded libraries were prepared using the NEB mRNA stranded library prep Kit (New England Biolabs).