GSM8893300: WT, CTLA-4, GEX; Mus musculus; RNA-Seq
At day 20 after intracranial injection of GL261 cells, deep cervical lymph nodes were collected and dissociated into a single-cell suspension. Live immune cells were enriched and sorted by flow cytometry for downstream analysis. Single-cell libraries were prepared using the 10x genomics  Chromium Next GEM single cell 5' V2 (10x Genomics) following the manufacturer's instructions.