GSM9235669: D1_Monocytes_Control Ab; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNeasy Micro Kit (Qiagen) Total RNA was quantified with the Qubit RNA HS Assay Kit (Thermo Fisher Scientific) and quality was assessed using RNA ScreenTape on TapeStation 4200 (Agilent Technologies). cDNA library was generated from 2 nanograms of total RNA using the Smart-Seq V4 Ultra Low Input RNA Kit (Takara). 150 picograms of cDNA were used to make sequencing libraries with the Nextera XT DNA Sample Preparation Kit (Illumina). Libraries were quantified with the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific) and the average library size was determined using D1000 ScreenTape on TapeStation 4200 (Agilent Technologies). Libraries were pooled and sequenced on NovaSeq 6000 (Illumina) to generate 30 millions single-end 50-base pair reads for each sample.