GSM8720732: KYSE450-DMSO_L2_Q0057W0149; Homo sapiens; OTHER
The Hyperactive Universal CUT&TAG Assay Kit for Illumina Pro was employed to prepare DNA libraries for Illumina high-throughput sequencing platforms. A total of 1 × 10^5 input cells were harvested as input material Initially, cells were fixed using the cross-linking reagent, followed by the binding of 2 μg of specific antibodies (HMGB2, SREBF1, H3K27ac, H3K4me, H3K27me, GCR, and IgG antibody), respectively. Subsequently, the transposase enzyme was activated, leading to the cleavage of DNA at the targeted sites. The Tn5 transposase was then utilized to tag the DNA, introducing adapters for downstream analysis. After a series of washing steps to remove unbound fragments, the eluted material was subjected to PCR amplification to enrich the targeted DNA fragments. The amplified DNA was finally sequenced to obtain high-throughput data for further analysis.