RNA-seg of penaeus vannamei
mRNA was enriched with magnetic beads, fragmented, and reverse-transcribed into cDNA using random primers. After end-repair and A-tailing, Illumina adapters were ligated. 400500 bp fragments were size-selected with AMPure XP beads, PCR-amplified for 15 cycles, purified, and quantified on an Agilent Bioanalyzer.