GSM8995307: SKM1, H3K27ac, 5azaC, rep1; Homo sapiens; OTHER
CUT&Tag was performed using the CUT&Tag-IT Assay Kit (Active motif, 53160) following the manufacturer's instructions. 0.2 million cells were used per sample. For 5-azaC-treated CUT&Tag profiling of H2AK119ub and H3K27ac, 4,000 drosophila nuclei (Active motif, 53168) were added as a spike-in control. Primary antibodies used included 1 μL H2AK119ub antibody (Cell Signaling Technology, 8240S), 1 μL H3K27ac antibody (Active motif, 39034) or 0.25 μL SRSF2 antibody (Abcam, ab11826). Libraries were constructed according to the kit protocol and sequenced on the Illumina NovaSeqX platform with pair-end 150 bp reads.