GSM9023406: Normal diet (ND) gene expression file; Mus musculus; RNA-Seq
Mice were euthanized and kidneys excised and single cell suspensions generated. Cells were also incubated with Total-SeqB hashing antibodies per manufacturer's directions (Biolegend). the hasing antibodies were: ND samples - B0301 (ACCCACCAGTAAGAC), DIO samples- B0302 (GGTCGAGAGCATTCA) and TRF samples - B0303 (CTTGCCGCATGTCAT). Samples were then FACS sorted for CD45+ cells using the BD S6 6-way sorter. A second set of samples were run without multiplexing, but FACs sorted for CD45+. 10X Genomics