Illumina HiSeq X paired end sequencing of SAMD00827023
Plant samples were frozen with liquid nitrogen, and crushed using a TissueLyser II (QIAGEN). RNA was purified using the Invitrogen Plant RNA Reagent and the Direct-zol RNA Kit (Zymo Research, R2052). Using the purified RNA as a template, cDNA synthesis was performed with the SMART-Seq HT Kit (Takara) and purified using AMPure XP beads (Beckman). Tagmentation and library preparation were carried out using the Nextera XT DNA Library Kit (Illumina).