PacBio HiFi of Megachile mendica: adult female
From total HMW DNA, we prepared a single SMRTbell library using the SMRTbell 3.0. library preparation kit following manufacturers protocols for low input samples. DNA was sheared to 15-20 kb using a Megaruptor 3 (Diagenode, Denville, NJ, USA) prior to library preparation, and the final library was sized with Ampure PB beads to remove fragments less than 3 kb in length. The entire library was quantified using Qubit HS DNA reagents, sized on a Agilent Fragment Analyzer to determine molar concentration and then bound and loaded for sequencing on a single cell on a Pacific Biosciences system.