Adipose tissue in the groin
In this study, we conducted transcriptomic analysis to evaluate the effects of adropin treatment on polycystic ovary syndrome (PCOS) in murine models.  Inguinal adipose tissue samples were collected from both adropin-treated and untreated PCOS mice.  Total RNA was extracted using the RNeasy Lipid Tissue Mini Kit (QIAGEN), ensuring the isolation of high-quality RNA suitable for sequencing.  Library preparation was performed using the TruSeq RNA Sample Preparation Kit v2 (Illumina), which involves purification of poly-A containing mRNA, followed by fragmentation and cDNA synthesis.  Sequencing was carried out on an Illumina NovaSeq 6000 platform to generate paired-end reads.  This approach allows for a comprehensive assessment of the molecular changes induced by adropin treatment in the adipose tissue of PCOS mice, providing insights into the potential therapeutic mechanisms of adropin in modulating adipose tissue function and metabolism associated with PCOS