GSM8718002: 3_Ubc13 flox/flox Alb positive_1; Mus musculus; RNA-Seq
RNA was extracted from liver tissues and cells using the Hybrid-R kit (Geneall, Cat. 305-101), according to the manufacturer's instructions. Murine primary hepatocyte RNA sequencing was performed with the QuantSeq 3' mRNA-Seq Library Prep Kit (Lexogen) following the manufacturer's protocol. cDNA libraries were prepared with an oligo-dT primer for first-strand synthesis and a random primer for second-strand synthesis, then purified using magnetic beads. Final libraries were amplified to include adapter sequences and purified post-PCR. Sequencing was performed as single-end 75 bp reads on an Illumina NextSeq 550.  Reads were aligned with Bowtie2 to the genome or transcriptome. Transcripts were assembled, and differential expression was analyzed with EdgeR using TMM+CPM normalization. Gene classification utilized DAVID and Medline databases, with data visualization by ExDEGA (Ebiogen Inc.).