GSM8458758: K562, WT, 2, VP:CTCF_1; Homo sapiens; OTHER
4C template preparation was performed as previously described in PMID: 31351925. In brief, ten million cells per sample were cross-linked in 2% formaldehyde. Chromatin was digested in situ with DpnII (NEB, cat. no. R0543) followed by dilution and ligation. Subsequently, crosslinks were removed by Proteinase K treatment, followed by template trimming using Csp6I (CviQI, second cutter, Thermo Scientific, cat. no. ER0211) and re-ligation. Indexed Illumina sequencing adapters were introduced to ligation fragments of interest with a two-step PCR strategy using ELT PCR System (Roche, cat. no. 11681842001).