GSM9211274: CTL Rep1; Mus musculus; RNA-Seq
RNA was extracted from ventricular tissue below the ligature using Trizol reagent per manufacturer's protocol (Thermo, #15596-026) and dissolved in RNAse-free water. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification (Novogene)