PacBio Kinnex sequencing of Cheney Lake, AK threespine stickleback abductor muscle
RNA was extracted using the Quick-RNA miniprep kit (Zymo, R1055). Kinnex full-length RNA libraries were generated per manufacturers recommendations (PacBio, 103-072-000). Samples were sequenced on the Revio platform on SMRT Cells 25M with Revio Chemistry V1 (PacBio, 102-817-900) with Adaptive Loading and 30-hour movies.