GSM5677833: GMP - rep12; Homo sapiens; Bisulfite-Seq
DNA was extracted from the sorted cells by using a DNA-extraction kit (Qiagen) and then bisulfite treated using an EZ DNA methylation kit (Zymo Research). Bisulfite-modified DNA sequencing libraries were generated using the EpiGenome kit (Epicentre) according to the manufacturer's instructions. Bisulfite-modified DNA libraries were sequenced using NovaSeq 6000 system.