Census-seq low-coverage WGS of 12-donor neural progenitor cell validation village W3, passage 1, day 5, well 3, untreated control
Twelve human ESC-derived neural progenitor cell donor lines were pooled at equal proportions into cell villages to validate village-based neural progenitor cell fitness assays. Post-baseline samples were maintained in triplicate wells across serial passages and harvested for genomic DNA extraction and Census-seq low-coverage whole-genome sequencing to estimate donor fractions and proliferation dynamics from genotype-informative variants.