GSM9103914: scRNA-seq of B16F10 ZDHHC13 tumor, mouse 2; Mus musculus; RNA-Seq
Tumors were dissociated into single-cell suspensions. CD45+ immune cells were isolated using flow cytometry-based sorting. Total RNA was extracted following the 10x Genomics Chromium workflow. For single-cell RNA-seq, libraries were prepared using the 10x Genomics Chromium Single Cell 3ʹ protocol (Document #CG000204), targeting 10,000 cells per sample. cDNA synthesis and barcoding were performed on the Chromium Controller. After library prep and quality control (Bioanalyzer, Q-cycler), samples were sequenced on an Illumina NovaSeq 6000 platform (28 bp Read 1, 91 bp Read 2).