GSM8604699: Multiplex; Mus musculus; RNA-Seq
Dissected tumors were dissociated in GentleMACS device with Miltneyi Biotec Mouse Tumor Dissociation Kit (product 130-096-730). All tumors of a given experiment were processed together. The cell suspensions were then enriched for live cells with Miltenyi Biotec Dead Cell Removal Kit (130-090-101). Cells were then fixed with Parse Biosciences Cell Fixation Kit v2 and stored at -80 ºC for 2-6 months depending on the cell-line. Thawed cells were counted with propidium iodide staining on a Nexcelom cell counter. Instructions and reagents for the Parse Biosciences Evercode Whole Transcriptome v3 for 100k Cells were used. Two wells with a total of 5k cells were used per sample. Three rounds of combinatorial barcoding were performed. Overall, the use of the platform results in sequencing data files (fastq) containing reads from single cells from all samples.