GSM8966956: LM22, LD; Mus musculus; RNA-Seq
The gastrocnemius muscle was incubated with RNAlater (Thermo Fisher Scientific) for several hours according to the manufacturer's instructions and snap-frozen in liquid nitrogen. Frozen gastrocnemius muscle was homogenized twice for 30 sec in TRIzol reagent (Thermo Fischer Scientific) with 5-mm- and 3-mm-diameter stainless beads using a desktop bead Crusher Shakeman 6 (Bio Medical Science Inc., Tokyo, Japan) at 3500 rpm. Poly(A)-enriched stranded RNA sequencing was carried out using TruSeq stranded mRNA kit by the NGS core facility at the Research Institute for Microbial Diseases of Osaka University on Illumina NovaSeq X with 101-bp paired-end reads.