ONT genome sequence of Xanthomonas oryzae pv. oryzae JS1
Genomic DNA is extracted, optionally sheared (>20 kb), then end-repaired and dA-tailed.Sequencing adapters (motor protein-linked) are ligated to DNA ends using T4 ligase.Purified libraries are mixed with sequencing buffer (fuel enzymes/voltage drivers) and loaded onto flow cells.DNA strands pass through nanopores via voltage; motor proteins regulate translocation.Current changes are translated into real-time base calls, enabling long-read, amplification-free sequencing.