GSM9211073: Mutant day 38 IL1B treated; Homo sapiens; RNA-Seq
Total mRNA was isolated using an Extraction Mini Kit (Qiagen) following the recommendations of the manufacturer Libraries were generated using Chromium Next GEM Single Cell 3' and Next GEM Chip G Single Cell Kits (10x Genomics) at the genomics platform at the Institute du Cerveau. The quality of the libraries was assessed using the High Sensitivity D5000 ScreenTape Assay for TapeStation Systems (Agilent), and the libraries were sequenced on NovaSeq 6000. The minimum requested coverage was 50,000 reads per cell.