Standard NextSeq 500 paired-end Sequencing; ChIP-seq for the cofactor Chip in Drosophila melanogaster embryos at 2-3h, 2-4h, 6-8h and 10-12h after egg-laying
ChIP-seq for the cofactor Chip in Drosophila melanogaster embryos at 2-3h, 2-4h, 6-8h and 10-12h after egg-laying
All fly lines were maintained in a standard cornmeal-agar medium and grown at 25°C. ; The collections were aged at 25°C to the corresponding time window (2-3h, 2-4 h, 6-7h, 6-8 h, 10-12 h), and exposed to blue light for the indicated durations (as stated in the results).  Embryos were fixed in 1.8% formaldehyde for 15 minutes, with the exception of ChIP-seq and CUT&Tag using the rabbit anti-Chip antibody and guinea pig anti-CBP antibody, where 3% formaldehyde for 30 min was used. ; Chromatin Extraction. ; Chomatin IP was performed as described in Bonn et al. 2012 (doi: 10.1038/ng.1064). ; ChIP-seq libraries were prepared following the manufacturer's manual of NEBNext Ultra II DNA Library Prep Kit for Illumina E7645S.