GSM8967401: SARS-CoV-2 S transfected, the positive control, rep3; Homo sapiens; RNA-Seq
Total RNA was isolated using a TRIzol total RNA extraction kit (TIANGEN,Cat.No.DP424), which yielded > 2 μg of total RNA per sample. RNA quality was examined by 0.8% agarose gel electrophoresis and spectrophotometry. High-quality RNA with a 260/280 absorbance ratio of 1.8-2.2 was used for library construction and sequencing. Poly (A) RNA is purified from 1 μg total RNA using Dynabeads Oligo (dT) . Then the poly(A) RNA was fragmented and reverse-transcribed to create the cDNA. Amplify cDNA for the synthesis of the second chain of cDNA. Purify cDNA products by AMPure XP system (Beckman Coulter, Beverly, USA). After library construction, library fragments were enriched by PCR amplification and selected according to a fragment size of 350-550 bp. The library was quality-assessed using an Agilent 4200 Bioanalyzer (Agilent, USA).