GSM8457912: N2, Rep1; Caenorhabditis elegans; RNA-Seq
Total RNA was isolated with TRIzol (Invitrogen) from worms homogenized with TissueLyser II (QIAGEN), followed by purification with RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions. After rRNA subtraction, RNA-seq libraries were prepared by Novogene and sequenced using Illumina Novaseq 6000 (PE150). For cDNA synthesis, isolated RNA was treated with DNase I (Invitrogen) at 37°C for 30 min, the enzyme was inactivated at 65°C for 10 min, and reverse transcription was performed using a random hexamer with SuperScript III First-Strand Synthesis System (Invitrogen) according to the manufacturer's instruction.