GSM9680363: IDG-SW3 treated with Nutlin-3a [10 μM] for 4 hours, rep 2; Mus musculus; OTHER
For scRNA-seq, the single-cell suspension was immediately loaded onto the Chromium Controller (10x Genomics) for droplet generation, cell barcoding, and reverse transcription. For scRNA-seq, libraries were constructed using the Chromium Single Cell 5' Reagent Kit (10x Genomics) following the manufacturer's protocol. For CUT&Tag, libraries were constructed using the Hyperactive Universal CUT&Tag Assay Kit for Illumina (Vazyme) following the manufacturer's instructions.