GSM9679281: ARID_Domain_Input_2; Homo sapiens; RIP-Seq
THP-1 macrophages were nucleofected with ARID5A domain and domain deletion constructs with V5 tags. Cells were pelleted and IP samples were generated by immunoprecipitation wtih an anti-V5 antibody. Inputs and IP samples were then lysed with Trizol and RNA was extracted using phenol/chloroform. For RNA samples, libraries were constructed using the Illumina Stranded mRNA Ligation preparation kit. In brief, equal amounts of RNA are subjected to polyA selection and subsequent fragmentation. Samples are reverse transcribed and adapters are ligated. The products are then purified and amplified for sequencing.