RNA-seq of Hepar Mus musculus: GAA-gavaged db/db mice
Total RNA was isolated from mouse liver using RNeasy Plus Mini Kit (QIAGEN, Duesseldorf, Germany) according to the manufacturers instructions. RNA-seq libraries were prepared using Illumina TrueSeq Stranded Total RNA Library Prep (Illumina, CA, USA). Samples were sequenced on Novaseq 6000 (Illumina, CA, USA) using paired-end protocol.