Single-cell RNA-sequencing of CD138- and CD138+ cells from bone marrow of patients with high-risk Smoldering Myeloma enrolled in Ixazomib, Lenalidomide and Dexamethasone Clinical Trial
Bone marrow were isolated using 1X red blood cell lysis buffer (Thermo Fisher). CD138- immune cells were magnetically sorted (Miltenyi Biotec) and the Chromium Single Cell 5' Gene Expression and V(D)J enrichment kit by 10X Genomics were used for library preparation. Libraries prepared with 5' technology were sequenced on a NovaSeq 6000 instrument at the Genomics Platform of the Broad Institute of MIT & Harvard.