GSM8893188: DTX1_KO, replicate 1, scRNA; Mus musculus; RNA-Seq
GC B cells were isolated from mice spleens at day 10 post-immunization using the Germinal Center B Cell (PNA) MicroBead Kit (Miltenyi Biotec, #130-110-479). Briefly, 10,000 cells/sample were loaded on the Chromium Controller. 10X libraries for scRNA-seq were prepared using chromium Next GEM Chip K Single Cell Kit, (PN-1000287) following the manufacturer's instructions.