GSM9022111: 48R.F.CTL Liver; Mus musculus; RNA-Seq
Tissues were harvested and snap frozen in liquid nitrogen. RNA was extracted using the PureLink RNA mini kit Total RNA was submitted to the University of Wisconsin-Madison Biotechnology Center Gene Expression Center (RRID:SCR_017757) & DNA Sequencing Facility (RRID:SCR_017759) for RNA quality assessment on an Agilent Biomek Plate Reader (A260/A280) and Agilent 4200Tapestation (RIN). RNA libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit Illumina (New England Biolabs GmbH, Frankfurt, DE) with a 500ng total RNA input.