NGS of Ceratitis capitata intestinal microbiota
Bacterial 16S ribosomal DNA amplicons were carried out to investigate the impact of P.lilacinum inoculation on intestinal microbiome profiling of V8-GSS C. capitata adults. The sequencing of the 16S rRNA gene V4 variable region was performed with PCR primers U515F (GTGCCAGCMGCCGCGGTAA) and 805R (GGACTACHVGGGTWTCTAAT) . To prepare illumina DNA library, samples were pooled together (equal proportion) based on their concentrations and molecular weight, and then purified using calibrated Ampure XP beads.