GSM8967472: LNCaP, shp300/CBP, H3K27ac ChIP; Homo sapiens; ChIP-Seq
PCa cells were crosslinked with 1% formaldehyde (Thermo fisher, Cat#28908)) for 10min at room temperature with gentle rotation and then quenched for 5min with 0.125M glycine. Chromatin was sonicated to an average length of 200–600 bp using an E220 focused-ultrasonicator (Covaris). Drosophila S2 cells were used as a spike-in control to normalize ChIP signals, following the protocol provided by Active Motif. ChIP–seq libraries were prepared from 3–5ng of ChIPed DNA using NEBNext Ultra II DNA Library Prep Kit (NEB, E7645S), according to the manufacturer's instructions.