DNA-Seq of Bolbitis angustipinna : fern leaves
A total amount of 0.2 g DNA per sample was used as input material for the DNA library preparations. Sequencing library was generated using NEB Next Ultra DNA Library Prep Kit for Illumina (NEB, USA) following manufacturers recommendations and index codes were added to each sample. Briefly, genomic DNA sample was fragmented by sonication to a size of 350 bp. Then DNA fragments were endpolished, A-tailed, and ligated with the full-length adapter for Illumina sequencing, followed by further PCR amplification. After PCR products were purified by AMPure XP system (Beckman Coulter, Beverly, USA), DNA concentration was measured by Qubit3.0 Flurometer (Invitrogen, USA), libraries were analyzed for size distribution by NGS3K/Caliper and quantified by real-time PCR (3 nM