ITS amplicon seq of fungal: surrounding soil
DNA was extracted from 0.5 g of soil with the PowerSoil DNA Isolation kit (MoBio Laboratories, Carlsbad, CA, USA). After DNA concentrations were quality checked and quantified, all samples were stored at -80C until sequencing analysis. The library construction and sequencing of  ITS gene were performed1. The universal primer sets,  ITS1F and ITS2R for the fungal ITS1, were used in this study