Single-nucleus RNA-Sequencing of whole livers from Four Core Genotype (FCG) mice
Single-nucleus RNA-Sequencing of whole livers from Four Core Genotype (FCG) mice
Livers were dissected and flash frozen in liquid nitrogen then subsequently stored at -80 degrees C.  Frozen livers were pulverized with a cryoprep, and a portion of frozen liver powder was lysed in a hypotonic PBS-based buffer with Igepal. During cell lysis, samples were dounced to liberate nuclei and subsequently fixed with 85% MeOH and DTSSP.  Fixed nuclei were distributed across wells of a 96-well plate, with individual mice indexed by RT well, and subjected to sci-RNA-seq3.