GSM9377767: low-grade serous ovarian carcinoma (VOA-3448), 3 replicates, 46 compounds, 2 conc., 24h, multiplexed; Homo sapiens; RNA-Seq
Cells were lysed using in house lysis buffer as described in Li et al., 2023 (Methods in Molecular Biology) The next-generation sequencing (NGS) library was prepared from frozen cells, with cell lysis and reverse transcription performed directly in the well (384 well format), circumventing the need for RNA extraction. During reverse transcription, firststrand cDNA was labelled with well-specific barcodes (384 unique sequences) allowing samples to be pooled in a single tube. The pool was then purified, amplified, and sonicated, and the resulting fragments were converted into an Illumina-compatible NGS library as per Li et al., 2023 (NextSeq 500)