T. asperellum ONT read (peptaibol)
Genomic DNA from Trichoderma asperellum was extracted using the Qiagen DNeasy PowerSoil Pro kit according to the manufacturers protocol. The DNA was quantified and normalized to 50 ng/L using Qubit 6. The sequencing library was prepared using the Oxford Nanopore Technologies Ligation Sequencing Kit (SQK-LSK109) following the standard protocol. A 1D single-read sequencing protocol was applied, and the samples were loaded onto a MinION flow cell for sequencing. The run was conducted for approximately 72 hours to ensure sufficient read depth and coverage. Basecalling was performed using Dorado (version 0.8.1), and the resulting fastq files were used for downstream analysis