112724 Fecal Sample from Symptomatic Coccidia-positive Black footed ferret (Zoey)
Genomic DNA was extracted using the ZymoBIOMICS-96 MagBead DNA Kit (D4302, Zymo Research, Irvine, CA). Shotgun metagenomic libraries were prepared using the Illumina DNA Prep Kit with 10 bp unique dual indexes, following the manufacturers protocol. Libraries were quantified with Qubit and pooled in equimolar amounts. Final library pools were quantified using qPCR and sequenced on either the Illumina NovaSeq X platform.