scRNA GEX for DFCI2, tumor sample: GBM1.DFCI2.d0.S.TS.L1_C2.v1__GBM1.DFCI2.d0.S.TS.L1_C3.v1
Tissue was processed fresh and droplet-separated using the Chromium Single Cell 5′ v.2 Reagent Kit (10X Genomics) with the 10x Genomics microfluidic system creating cDNA library with individual barcodes for individual cells. Barcoded cDNA transcripts from patients were pooled and sequenced using the Illumina's NovaSeq 6000 Sequencing System.