GSM9022245: THP-1, ad, rep.1; Homo sapiens; RNA-Seq
Total RNA extraction from cell lysates using Trisol reagent (Thermo Fisher Scientific) and PureLink RNA Micro Kit (Invitrogen).  RNA quality/quantity assessment via BioAnalyzer and RNA 6000 Nano Kit (Agilent Technologies). PolyA RNA isolation using Dynabeads® Oligo(dT) magnetic beads (Invitrogen).  Library preparation with NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB).  Library quantification using Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific).  Fragment length distribution analysis with Agilent High Sensitivity DNA Kit (Agilent Technologies).