GSM8893064: PANC-1, siHDAC5; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutesAfter auenching and cell livsis, chromatin was sonicated using a E220 focused-ultrasonicator (Covaris). ChIP-Seq libraries were prepared using the KAPA HTP Library Preparaion Kit comoemented wih NEXTflex DNA Barcodes from Bioo Scientific. 10ng of DNA was used as starting material for input and ip samples. Libraries were amplified using 13 cycles on the thermocycler. Post amplification libraries were size selected at 250-450bp in lenath using Agencourt AlMPure XP beads from Beckman Coulter. Libraries were validated using the Agilent High Sensitivity DNA Kit.