WGS DNA-Seq of TLR4-edited Raw264.7 cells
Genomic DNA was extracted from transfected Raw264.7 cells using QuickExtract DNA extraction solution. The cell lysate was heated in a Veriti thermal cycler (Thermo Fisher Scientific, Waltham, MA, USA) at 65oC for 10 min followed by 98oC for 5 min. Genomic DNA was subjected to whole-genome next-generation sequencing (WG-NGS). Genomic DNA was QCed, used to construct the NGS library (Illumina Phix control library), and sequenced on Illumina NextSeq 1000 (Illumina, Inc., San Diego, CA, USA; S/N: VL00482)