GSM9312194: Ven/aza treated BM stroma gene expression data (NGO7013A29); Mus musculus; RNA-Seq
Bone marrow cells were isolated by centrifugal flushing at 2500g for 1 min. BM was enzymatically digested with collagenase-type I (3mg/ml) and dispase (neutral protease, grade 2: 4mg/ml). BM cells also underwent RBC lysis protocol. Stromal cells were sorted based on CD45-/CD71-/Ter119- gating strategy scRNA-seq 10X 3' Gene Expression with Cell Multiplexing Oligo