CRISPRi-seq of Streptomyces albidoflavus J1074 transporters in minimal media
100-200 micorgrams of bacterial gDNA was digested with 100U of SbfI. Fragments 3-5kb in length were purified by agarose gel extraction and used as template to produce PCR amplicons. PCR amplicons were purified by agarose gel extraction and assessed by qubit and tapestation.