GSM9351926: CNP_NP_20; Mus musculus; RNA-Seq
Tissue was homogenized using a mini hand-held homogenizer (LX140MHH, Labdex) and  RNA was harvested using the RNeasy mini plus kit (Qiagen) Polyadenylated mRNAs were reverse transcribed using barcoded primers with UMI and template switching to generate full-length cDNA, which was subsequently amplified. For library construction, amplified cDNA was processed using the Illumina-compatible library preparation workflow with library construction kit (10X Genomics). Final libraries were quality-checked and sequenced on an Illumina NovaSeqX Plus platform.