GSM9351008: F121-9 WT, NPC status, replicate 1; Mus musculus; RNA-Seq
Direct-zol RNA MiniPrep (Zymo research R2050) was used according to the manufacturer's instruction. Starting from approximately 300ng total RNA, ribosomal RNA was depleted using NEBNext® rRNA Depletion Kit v1 (NEB E6310). The entire resulting RNA was used for 1st strand synthesis by NEBNext Ultra II RNA First Strand Synthesis Module (NEB E7771), following “Directional RNA-seq Workflows“. Second strand was synthesized using NEBNext Ultra II Directional Second Strand Synthesis Kit (NEB E7550) then cDNA was made into sequencing library using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB E7645).