GSM9022251: Tendon cells,7 days post-injury,negative; Mus musculus; RNA-Seq
We have established a controlled longitudinal cohort comprising Gli1-CreERT2; Ai9 reporter mice with tamoxifen induction (n = 24, equal sex distribution). Mice were stratified into three experimental groups: uninjured controls (n = 8) and tenotomy-injured cohorts at 7- and 21-days post-injury (n = 8/group). All samples were collected and subjected to FACS for isolation of Gli1⁺ and Gli1⁻ cells. These sorted cells were lysed and prepared for scRNA-seq. RNA libraries  were prepared with Chromium Single cell 3' Reagent v3 Kits according to the manufacturer's protocol.