GSM8604696: MGE-derived neuron, PT2, replicate1; Homo sapiens; RNA-Seq
Day 80 neuronal cultures were dissociated with Accutase containing 10 units/mL of papain (Sigma-Aldrich) for 8 minutes at 37°C, resuspended in DPBS (Gibco), mixed with 0.5% bovine serum albumin (Sigma Aldrich) and 50 units/mL DNase I (Sigma Aldrich), and passed through a 35 µm mesh cell strainer. Cells were stained with ReadyProbes™ Cell Viability Imaging Kit, Blue/Red (Invitrogen) following manufacturer's guidance, and then dispensed into the nano-well plates of the ICELL8® cx Single-Cell System (Takara). Wells containing a single nucleus were automatically and manually selected using the ICELL8 cx CellSelect v2.5 Software (Takara). The sequencing library was prepared using the SMART-Seq ICELL8 application kit (Takara) following manufacturer's instructions.