RNA-Seq of mus musculus: adult male lung
Total RNA was extracted from lung tissues after anatomy four samples were randomly picked from each group. The concentration and purity of the extracted RNA were assessed using the ultramicro spec- trophotometer (Nanodrop 2000), and the integrity of the RNA was examined using agarose gel electrophoresis. A magnetic bead with Oligo (dt) was used for base pairing with polyA to isolate mRNA for tran- scriptome analysis. mRNA sequencing was based on the Illumina Nocaseq 6000 sequencing platform for all mRNA in lung tissue. The Illumina TruseqTM RNA sample prep it method was used for library expansion.