Illumina sequencing of Buchnera aphidicola: isolated from single gall colony of Eriosoma harunire
Genomic DNA quantity was assessed using NanoDrop 2000c Spectrophotometer and Qubit 2.0 Fluorometer (Thermo Fisher Scientific). Genome sequencing of Buchnera aphidicola from three gall-forming aphids was performed using Oxford Nanopore long-read sequencing, with error correction from Illumina TruSeq short-read data. Illumina libraries were fragmented (~350 bp) using a Covaris M220 ultrasonicator and sequenced on the Illumina NextSeq 550 system at the Functional Genomics Facility, National Institute for Basic Biology, Okazaki, Japan.