WGS of P. brasiliense
For the purpose of genome sequencing, DNA was extracted from pure bacterial cultures obtained by growing the strains on TSA medium. Per isolate approximately one inoculation loop (10__l) of bacterial slime was collected from multiple colonies. The DNA extraction was performed by using the Wizard Magnetic DNA Purification System for Food (Promega, Leiden, the Netherlands) according to the manufacturers protocol. The NEBNext Ultra II DNA Library Prep kit for Illumina (cat# NEB #E7645S/L) was used to prepare sequencing libraries. Fragmentation of the DNA using the Biorupor Pico (Diagenode), ligation of sequencing adapters, and PCR amplification of the resulting product was performed according to the procedure described in the NEBNext Ultra DNA Library Prep kit for Illumina Instruction Manual. The quality and yield after sample preparation was measured with the Fragment Analyzer (Table 2 and Appendices). The size of the resulting product was consistent with the expected size of approximately 500-700 bp. Clustering and 150bp paired-end DNA sequencing using the NovaSeq6000 was performed according to manufacturer's protocols. A concentration of 1.1 nM of DNA was used.