GSM8720038: NC_2; Homo sapiens; RNA-Seq
Each sample underwent treatment with RQ1 DNase (M6101, Promega, USA) to eliminate DNA from total RNAs. Ribosomal RNAs were depleted using the Ribo-off™ rRNA depletion kit (N406, Vazyme, China). VAHTS® Universal V8 RNA-seq Library Prep Kit for Illumina (NR605, Vazyme, China) was employed for RNA-seq library preparation. Fragmented RNAs were converted into double-stranded cDNA, followed by end repair, A tailing, and ligation to VAHTS RNA Multiplex Oligos Set 1 for Illumina (N323, Vazyme, China). The ligated products were then amplified, purified, quantified, and stored at -80°C prior to sequencing. The strand marked with dUTP (the 2nd cDNA strand) was not amplified, allowing for strand-specific sequencing. High-throughput sequencing was carried out using the Illumina Novaseq 6000 system with the PE150 model.