GSM9682831: ago1∆ pir1∆_Biorep3; Schizosaccharomyces pombe; RNA-Seq
Total RNA isolated using the MasterPure Yeast RNA Purification Kit following the manufacturer's protocol For RNA Seq-Sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina according to the manufacturer's protocol and analyzed using an Agilent 4200 TapeStation system (Agilent). Total RNA isolated was first ribodepleted using the Ribo-Zero gold plus for humans (Illumina) supplemented with probes against S. pombe ribosomal DNA (IDT) for library construction