RNA-seq of Chronic Lymphocytic Leukemia patients with and without t(14;19) samples, and normal mature B cells
RNA-seq of Chronic Lymphocytic Leukemia patients with and without t(14;19) samples, and normal mature B cells
10^6 leukemic B cells from CLL patients. B cells were purified by negative selection using RosetteSep™ B cell enrichment Cocktail (StemCell Technologies, Vancouver, Canada) Total RNA was extracted with the RNeasy Mini Kit (Qiagen, Hilden, Germany) and treated with DNase (Qiagen), according to the manufacturer's protocol Illumina Stranded mRNA Prep kit but replacing the messenger RNA capture phase with ribosomal RNA depletion performed with the Qiagen Qiaseq Fastselect HMR kit.