GSM9351913: CCA PDM217, control, rep3; Homo sapiens; RNA-Seq
Organoids were harvested for RNA extraction using PicoPure™ RNA Isolation Kit (#KIT0204, Applied Biosystems™) according to the manufacturer's instructions RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis