GSM8458379: P6ATM3; Mus musculus; RNA-Seq
Total RNA extraction using TRIZOL extraction reagent. mRNA molecules were purified from total RNA using oligo(dT)-attached magnetic beads. Library was validated on the Agilent Technologies 2100 bioanalyzer. The library was amplified with phi29 to make DNA nanoball (DNB) which had more than 300 copies of one molecular. The DNBs were load into the patterned nanoarray and single end 50 (pair end 100/150) bases reads were generated in the way of combinatorial Probe-Anchor Synthesis (cPAS).