GSM8655041: Patient AC-001-069 CLL treatment-naïve PD-1+; Homo sapiens; RNA-Seq
Previously frozen PBMC samples were thawed and stained with Live/Dead Fixable Near-IR Dead Cell Stain (Invitrogen) followed by antibodies directed against CD3, CD19, CD20, CD5, and PD-1. Cells were sorted into RLT buffer at the Emory University School of Medicine Flow Cytometry core using a BD FACSAria II. RNA isolation, library preparation, and sequencing were performed by the Emory University National Primate Center Nonhuman Primate Genomics Core at Emory University. SMART-Seq v4 Ultra Low Input RNA Kit with Illumina's Nextera XT DNA Library preparation kit