Targeted single-cell sequencing library of 312 amplicons across 45 myeloid genes, generated from a primary AML sample.
Single cell mutation analysis was performed on patient AML samples (blood or marrow mononuclear cells) to document the subclonal composition. We utilized the Tapestri Single Cell DNA plus Protein Sequencing Kit (PN6870 A1, MissionBio) and the Tapestri Single-cell DNA Myeloid Panel (MissionBio) for targeted sequencing. The Myeloid Panel consists of 45 genes with 312 amplicons, 1197 targets with 99% coverage. Single cells were encapsulated, lysed, and barcoded on the Tapestri instrument. A DNA library was prepared. DNA analysis and sequencing was performed by the University of California Irvine High Throughput DNA Sequencing Core (NCI Shared Resource) on an Agilent Bioanalyzer and Illumina NovaSeq 6000. A total of 294 million to 893 million reads were obtained for each sample. The sequences were analyzed using Tapestri Insights 2.2 software (MissionBio) to provide the characterization of the clonal mutations and distribution.