ScRNA-Seq of mus musculus: adult male colon
ScRNA-seq libraries were prepared using the DNBelab C Series High-throughput Single-Cell RNA Library Preparation Set (MGI, 940-001818-00), following a workflow that included single-cell suspension processing, droplet encapsulation, emulsion breakage, mRNA capture, reverse transcription, cDNA amplification, and purification. The amplified cDNA was sheared into 300-500 bp fragments, followed by indexed library construction according to the manufacturers protocol, with quality assessment performed using the Qubit ssDNA Assay Kit (Thermo Fisher Scientific) and Agilent Bioanalyzer 2100.