PacBio Kinnex sequencing of marine x freshwater F1 hybrid stickleback: 23wpf, Abductor muscle, 18C
RNA was extracted using the RNeasy Fibrous Tissue Mini Kit (Qiagen, 74704). Kinnex full-length RNA libraries were generated per manufacturers recommendations (PacBio, 103-072-000). Samples were sequenced on the Revio platform on SMRT Cells 25M with Revio SPRQ chemistry (PacBio, 103-520-200) with Adaptive Loading and 30-hour movies.