GSM8827139: Arsenic treated male brain; Drosophila melanogaster; RNA-Seq
We anesthetized CSB flies on ice and dissected 10 brains per sex after six days of exposure to control medium or medium supplemented with 250 µM NaAsO2 in cold Dulbecco's Phosphate-Buffered Saline (DPBS). The brains were flash frozen in a dry ice-ethanol bath, and stored at -80°C. We prepared the samples for snRNA-seq as described in the 10X Genomics Gene Expression (GEX) protocol (CGOOO338).