Metabarcoding analysis of whey fermentations performed using kombucha SCOBY
DNA was extracted from samples using the Qiagen Powerfoods kit, and the bacterial 16S V4-V5 region amplified using 515F and 926R fusion primers incorporating Illumina Nextera v2 indices and P5/P7 adapters. DNA concentrations normalized using Sequelprep plates. Pooled libraries sequenced on MiSeq with 2x300 bp chemistry.