Transcriptomic analysis identifies metabolic dysregulation in ARDS patients
Transcriptomic analysis identifies metabolic dysregulation in ARDS patients
Peripheral blood samples were collected from 6 healthy volunteers (Con group), 7 patients meeting the Berlin Definition of ARDS at the Intensive Care Units of Xishan People's Hospital of Wuxi City and Affiliated Hospital of Jiangnan University between January 2024 and December 2024.  Total RNA was extracted from PBMCs using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. RNA quality and quantity were assessed using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and agarose gel electrophoresis. RNA sequencing libraries were constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA) and sequenced on the Illumina NovaSeq 6000 platform (Illumina, San Diego, CA, USA) with 150-bp paired-end reads.  Total RNA was extracted from PBMCs using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. RNA quality and quantity were assessed using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and agarose gel electrophoresis. RNA sequencing libraries were constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA) and sequenced on the Illumina NovaSeq 6000 platform (Illumina, San Diego, CA, USA) with 150-bp paired-end reads. 