Plasmid library RTW574, p11-5_10xN_PAM-site4, treated with BrCas12b, replicate 1
Library design is described in Walton, R.T., Hsu, J.Y., Joung, J.K. et al. Scalable characterization of the PAM requirements of CRISPRCas enzymes using HT-PAMDA. Nat Protoc 16, 15111547 (2021). https://doi.org/10.1038/s41596-020-00465-2