SO_10327_YC2
Methylation sequencing libraries were prepared with Illumina-compatible SureSelectXT Methyl- Seq Target Enrichment System for Illumina Multiplexed Sequencing (Agilent Technologies, Santa Clara, CA, USA) at Genotypic Technology Pvt. Ltd., Bangalore, India. Briefly, 400ng-500ng of genomic DNA was sheared using Covaris S2 sonicator (Covaris, Woburn, Massachusetts, USA) to generate approx. fragment peak size between 150-200 bp. Here, the fragment size distribution was checked on Agilent 2200 TapeStation with D1000 DNA screen tapes and reagents (Agilent Technologies, Palo Alto, CA, USA). The fragments were end-repaired, adenylated and ligated to Illumina adaptors as per SureSelectXT Methyl-Seq Target Enrichment protocol