GSM8966466: siCLDN1-GIST-3; Homo sapiens; RNA-Seq
Total RNA was isolated from the GIST cell lines using the TRIzol reagent (RNAiso, Invitrogen, Carlsbad, CA, USA). The quantity and purity of RNA was estimated on a NanoPhotometer (Implen), and 800 ng of RIN ≥7 of total RNA was used for library construction using NEBNext® Poly(A) mRNA Magnetic Isolation Module and NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) according to the manu-facturer's instruction.