RNA-seq data to map the AtsR regulon in B. cenocepacia
RNA-seq data to map the AtsR regulon in B. cenocepacia
Overnight liquid cultures of each isolate, B. cenocepacia wild type and each mutant, that were grown at 37°C while shaking at 200 rpm in SCFM, were transferred to fresh media until the mid-exponential phase was reached. The cultures were subsequently diluted to a standardized culture to OD600nm 0.4 in 250 ml glass flasks. These cultures were further incubated for 18 hours before centrifugation at 4°C and the obtained cell pellets stored at −80 °C for a maximum of 1 week.  Cells were grown at 37°C while shaking at 200 rpm in synthetic CF medium (SCFM).  RNA extraction was outsourced to GENEWIZ Europe (Leipzig, Germany).  Library prep was outsourced to GENEWIZ Europe (Leipzig, Germany).