Illumina sequencing of Codonopsis javanica from Vietnam
Total genomic DNA was extracted from fresh leaf tissue using the Isolate II Plant DNA Kit (Bioline, UK) following the manufacturers instructions. DNA quality was evaluated by 1% agarose gel electrophoresis, and concentration/purity were measured with a NanoDrop spectrophotometer (ThermoScientific, USA). Approximately 500 ng of high-quality DNA, after quality control, was subjected to acoustic fragmentation on a Covaris S220 instrument, followed by end-repair, dA-tailing, adapter ligation, purification, and size selection to generate sequencing libraries. Libraries were PCR-amplified, quantified (including qPCR to estimate effective concentration), and pooled. Library preparation, QC, cluster generation, and sequencing were performed on an Illumina NovaSeq 6000 platform to produce paired-end 150 bp reads.