GSM8454399: E6.5 Epiblast,Total RNA,rep1; Mus musculus; RNA-Seq
The RNA-seq library was generated following the Smart-seq2 protocol. Samples were permeabilized in 5 µl lysis buffer containing 0.2% Triton X-100 for 30 minutes on ice. After centrifugation, 2 µl of the supernatant was transferred to a pre-cooled tube, and reverse transcribed into cDNA. The library construction was then performed using the One-step DNA Lib Prep Kit for Illumina V2 (Abclonal) according to the manufacturer's instructions.