GSM8859474: KMS11, control, ATAC-seq, biorep1; Homo sapiens; ATAC-seq
The cells were resuspended cold ATAC-Resuspension Buffer containing 0.1% NP40, 0.1% Tween20, and 0.01% Digitonin and incubated on ice for 3 min. The cells were then washed in RBS containing 0.1% Tween-20 at 500 g at 4 °C before subjecting the nuclei to the transposase reaction for 30 min at 37 °C. Termination of the reaction and DNA purification was performed using Zymo DNA clean and Concentrator kit. The purified DNA was amplified with NEBNext High-Fidelity 2x PCR Master Mix then purified and size selected twice with AMPure beads. Nextera Tn5 Transposase is employed for tagmentation.