GSM9644565: CLAD lung, Tumor segment, ROI C3P2; Homo sapiens; OTHER
Paraffin-embedded human lung tissue sections (5 um) were placed on GeoMx slides. Tissue was deparaffinized and rehydrated. RNA targets were hybridized with the NanoString GeoMx Human Whole Transcriptome Atlas (WTA) probe panel. Fluorescently labeled antibodies against CD31, PanCK, and CD45 were applied for morphology-guided region of interest (ROI) selection using the GeoMx Digital Spatial Profiler (DSP). UV-cleaved oligonucleotide tags from selected areas of illumination (AOIs) were collected, indexed, and PCR-amplified to generate sequencing libraries using the NanoString GeoMx NGS Library Prep kit per manufacturer instructions. Panel: (v1.0) Human NGS Whole Transcriptome Atlas RNA.