GSM8510492: ADSC, mock, rep2; Mus musculus; RNA-Seq
Novogeno used standard extraction methods to isolate RNA from tissues or cells, followed by strict quality control of the RNA samples. The primary quality control method involves the Agilent 2100 Bioanalyzer, which accurately assesses RNA integrity. After completing library construction, initial quantification was performed using the Qubit 2.0 Fluorometer, and the library was diluted to 1.5 ng/μL. The insert size of the library was then assessed using the Agilent 2100 Bioanalyzer. Once the insert size met expectations, qRT-PCR was used for accurate quantification of the library's effective concentration.