GSM9023126: Yy1WT_Rep2; Mus musculus; ATAC-seq
ATAC-seq libraries were prepared from 1×10⁵ cells using the Active Motif ATAC-seq Kit (cat#53150), following the manufacturer's instructions. Briefly, cells were washed with 1 mL of ice-cold PBS and lysed with 100 μL of ice-cold ATAC Lysis Buffer. Cells were spun down at 500 x g for 10 minutes at 4°C, and cell pellets were incubated in 50 μL of Tagmentation Master Mix at 37°C for 30 min in a thermomixer set at 800 RPM. Cells were incubated with Tagmentation Master Mix, and tagmented DNA was purified via DNA columns. Indexing was performed by 12-cycle PCR enrichment using Illumina UDP Indexes, followed by double-sided size selection (L=1.1; R=0.6) with Ampure beads (KAPA, Roche). Library size and quality were assessed on an Agilent 2100 Bioanalyzer with a High Sensitivity DNA Kit and quantified by qPCR. Equimolar libraries were sequenced as 100 bp paired-end reads (PE100) on a NovaSeq X (Illumina), generating ~70M fragments per sample.