RNA-Seq of 3D Real Microgravity - 7Days (1)
RNA sequencing was performed on human AC16 cardiomyocytes cultured as 3D cardiac organoids on polycaprolactone (PCL) scaffolds coated with Matrigel. RNA quality was assessed using an Agilent Bioanalyzer (Pico chip). Libraries were prepared and sequenced using paired-end 2  100 bp reads on an Illumina NextSeq 2000 platform to evaluate gene expression under real microgravity.