GSM8966972: N2 + dietary restriction 1 (DR1), rep 1; Caenorhabditis elegans; RNA-Seq
On the day of the extraction, 1 ml of TriPure Isolation Reagent (Cat. 11667165001, Roche) was added to each tube. RNA was then extracted by using a column-based kit from Macherey-Nagel (Cat. 740955.250). RNA libraries were prepared for sequencing using standard Illumina NovaSeq 6000 protocols with polyA enrichment