RNA-seq in biological triplicates of U2OS WT cells treated with either DMSO or PARGi
RNA-seq in biological triplicates of U2OS WT cells treated with either DMSO or PARGi
Extracted molecule: total RNA DMEM (Sigma) supplemented with 10% FBS (Gibco) and penicillin-streptomycin (100 U/mL, Gibco) DMSO or 25µM PARGi inhibitor (PDD00017273, Sigma) for 4 days RNA was extracted using Direct-zol RNA MiniPrep Plus kit (Zymo Research) according to the manufacturer's instructions. The quality of the RNA was measured spectrophotometrically using NanoDrop 2000c (Thermo Scientific) and quality was analysed by RNA ScreenTape Assay and 4200 TapeStation System (Agilent). For RNA-seq, libraries were prepared using Illumina's TruSeq Stranded Total RNA library Preparation Kit according to Illumina's instructions