GSM9682505: GBe046s008_18; Homo sapiens; OTHER
Cells were harvested, washed in cold PBS, and resuspended in nuclear extraction buffer. Intact nuclei were incubated with primary antibodies targeting mSWI/SNF subunits (SMARCC1, SMARCB1, SMARCA4), CBFA2T3::GLIS2 fusion components (CBFA2T3, GLIS2), histone modifications (H3K4me3), or IgG isotype control, followed by secondary antibody and pA/G-Tn5 incubation. Tagmentation was activated by MgCl₂ at 37°C and resulting DNA fragments were purified. CUT&Tag libraries were prepared in-house using the CUT&Tag protocol (Kaya-Okur et al., 2019). Libraries were sequenced by Novogene on an Illumina instrument.