GSM8510425: SK-BR-3 cells, rep3; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIZOL.  1 μg of RNA for each sample was processed with RQ1 DNase to remove DNA. VAHTS mRNA capture beads were used to extract the mRNAs. Double-stranded cDNA was created from fragmented RNAs,After end repair and tailing,they were ligated to VAHTS RNA Multiplex Oligos Set 1 for Illumina. Prior to sequencing, the ligated products underwent amplification, purification, quantification, and storage at -80 °C. Strand-specific sequencing is possible because the second cDNA strand, which is designated with dUTP, is not amplified. The Illumina Library Preparation Kit previously utilized it for the purpose of generating customized RNA-seq libraries