GSM8993665: 7-100KOGDHL2930-2; Homo sapiens; RNA-Seq
RNA was extracted using the Qiagen RNeasy Kit Libraries for RNA-Seq were prepared with KAPA mRNA HyperPrep Kit. The workflow consists of mRNA enrichment and fragmentation, first strand cDNA synthesis using random priming followed by second strand synthesis converting cDNA:RNA hybrid to double-stranded cDNA (dscDNA), and incorporates dUTP into the second cDNA strand to maintain strand origin information. cDNA generation is followed by end repair to generate blunt ends, A-tailing, adaptor ligation and PCR amplification. Different adaptors were used for multiplexing samples in a half lane. Sequencing was performed on Illumina NovaSeq X Plus for PE 2x50 run. Data quality check was done on Illumina SAV. Demultiplexing was performed with Illumina Bcl2fastq v2.19.1.403 software.