RNA-seq of Lucilia ochricornis: whole third instar larva
We isolated whole body RNA using TRIzolTM (Life Technologies, Carlsbad, CA, USA). Each sample had its RNA isolated separately and then pooled in equimolar aliquot. Samples were shipped in dry ice for sequencing at at GenOne (Rio de Janeiro, RJ, Brazil). The  NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs Inc., Ipswich, MA, USA) was used for library construction. All samples underwent paired-end sequencing on the Illumina (San Diego, CA, USA) NovaSeq6000 platform (20M of PE reads 2x150pb).