Spatial transcriptome of maize leaves infected by P. polysora
In this project, Reverse transcription (RT), second-strand cDNA synthesis, adaptor ligation and a second RT was generated and libraries were constructed according to the performers protocol. Sequencing handles and indexes were added in an indexing PCR and the finished libraries were purified and quantified. Sequencing was performed on the Illumina NovaSeq 6000 with a sequencing depth of at least 50,000 reads per sopt(100m) and 150bp (PE150) paired-end reads (performed by Biomarker Technologies Corporation, Beijing, China).