GSM9643955: WT; Mus musculus; OTHER
Tissue slides and Visium CytAssist Spatial Gene Expression v2 Slides were used as input for the CytAssist instrument. Tissue sections were methanol-fixed, H&E-stained, imaged, and destained. Probe hybridization was then performed, followed by ligation of probe pairs bound to target transcripts. The resulting ligation products were transferred to the Visium slide using the CytAssist instrument, extended with UMI, spatial barcode, and partial Read 1 sequences, and then harvested. Libraries were amplified by sample index PCR, purified using SPRIselect, quantified by qPCR, quality-checked using the Agilent Bioanalyzer, and sequenced on the Illumina NovaSeq platform.