TCR Amplicon of human: healthy donor CD8+T cells spiked-in with 10% Jurkat cells
TIRTL-seq: Healthy donor PBMCs CD8+ T cells spiked in with 10% Jurkat cells, lysed and mRNA reverse transcribed using poly-A and random hexamers. cDNA amplified with TCR V segment and C segment multiplex primers in PCR1 which was then amplified with well specific dual-index primers containing Illumina adapters in PCR2. Libraries made with unique pairs of TCR-alpha and TCR-beta C-segment primers with inline barcodes (human abTCR-UD03).