GSM8967254: shCTRL tumor samples; Mus musculus; RNA-Seq
Mice were euthanized when primary tumors reached similar sizes in the cohorts analyzed (14 days post-implantation). Tumor were then excised, minced in RPMI and enzymatically digested in RPMI supplemented with 0.25 mg/ml collagenase (Merck C7657) and 50 U DNase in gentleMACS™ Dissociators with heaters, following the manufacturer's protocol. Cell suspensions were then filtered through a 70 µl strainer, treated with erythrocyte lysis buffer (ACK, Lonza) for 10 min on ice and then resuspended in RPMI for sorting. Cells were prepared for single-cell RNA sequencing using the 10x Chromium platform (10x Genomics) following the manufacturer's protocol for cell capture, barcoding, and cDNA library preparation.