GSM9207157: TMD8 cells, Rhosin treated for 10hrs, Replicate 2; Homo sapiens; RNA-Seq
RNA was harvested using miRNeasy Mini Kit (Qiagen) using QIAshredder homogenizers and on-column Dnase digests. Total RNA samples were processed at the DKFZ Core Facility using the Illumina TruSeq Stranded RNA Library Prep Kit with IDT-ILMN TruSeq DNA/RNA Unique Dual Index (UDI) adapters. Libraries were sequenced on an Illumina NovaSeq 6000 platform with paired-end 2×50 bp reads. Base calling and quality control were performed with standard Illumina software. WT and KI RhoA samples were generated at the NIH/CCR sequencing core unit and sequenced on NovaSeq 6000 S1 using Illumina® Stranded mRNA Prep and paired-end sequencing.