CRISPRi-seq of Streptomyces albidoflavus J1074 mreB, secY, tatA2, targetless
100-200 micorgrams of bacterial gDNA was digested with 100U of SbfI. Fragments 3-5kb in length were purified by agarose gel extraction and used as template to produce PCR amplicons. PCR amplicons were purified by agarose gel extraction and assessed by nanodrop.