GSM9568697: XE 2-4, mouse embryonic stem cells, RNA-seq, technical replicate3; Mus musculus; RNA-Seq
RNA-seq experiments performed with B6, D2, B6-13D2, and D2-13B6 used three independent cultures from the same cell line for each strain. For each library, 1 million cells were collected, and RNA was isolated using the NucleoMag RNA Kit (Machery-Nagel) and the KingFisher Flex purification system (ThermoFisher). RNA concentration and quality were assessed using the Nanodrop 8000 spectrophotometer (Thermo Scientific) and the RNA ScreenTape (Agilent Technologies). Libraries were constructed using the mRNA Library Prep Kit (Watchmaker) and assessed using the D5000 ScreenTape (Agilent Technologies) and Qubit dsDNA HS Assay (ThermoFisher), respectively. Libraries were sequenced 150 bp paired-end on an Illumina NovaSeq X Plus to a read depth of about 40 million reads per sample.