Shotgun metagenomic sequencing of placental fluid metagenome from an RBPT-positive aborted cow
Placental fluid from an RBPT-positive aborted cow was collected aseptically. DNA was extracted using the QIAcube Connect automated system and quantified with Qubit and NanoDrop instruments. Sequencing libraries were prepared following standard Illumina shotgun metagenomic protocols and sequenced on the Illumina NextSeq 500 platform to generate high-quality paired-end reads for microbial community profiling.