GSM9679746: DLPFC tissue from OCD subject 03 sample B; Homo sapiens; RNA-Seq
Standardized 0.3 cm3 samples were collected from the dorsolateral prefrontal cortex during deep brain stimulation electrode implantation. Tissue that would otherwise have been destroyed by cauterization was collected with a microdissector, placed into an Eppendorf tube on dry ice containing 250 uL Allprotect Tissue Reagent (Qiagen), transported immediately to the research laboratory, stored at -20°C for up to 7 days, and then processed for RNA extraction using the AllPrep RNA Mini Kit (Qiagen) according to the manufacturer's instructions. Purified RNA was stored at -80°C. RNA integrity was assessed using the Agilent 2100 BioAnalyzer System. All samples had RIN values greater than 6.5 (mean RIN = 7.5). Strand-specific RNA-seq libraries were generated after rRNA and globin depletion using the Globin-Zero Gold kit (Illumina) according to the manufacturer's instructions.