GSM8573313: Whole cells obtained after 5-days culture of EPCRHigh HSCs with R162, ATAC; Mus musculus; OTHER
After HSCs were cultured for 5 days, whole cells were collected by a centrifuge. Nuclei were isolated from collected cells, following the Nuclei Isolation for Single Cell Multiome ATAC + Gene Expression Sequencing protocol (10x Genomics, Pleasanton, CA), and then resuspended in a cell concentration of 5000 cells/µl. Cells were processed using a Chromium Next GEM Chip J Single Cell Kit (10xGenomics, PN-1000230), Chromium Next GEM Single Cell Multiome Reagent Kit A (10x Genomics, PN-1000284), and Chromium Next GEM Single Cell Multiome ATAC Kit A (10x Genomics, PN-1000281), following the manufacturer's User Guide. Targeted nuclei recovery was around 9,000. The single cell RNA and ATAC sequencing libraries were prepared using Dual Index Kit TT Set A (10x Genomics, 1000215) and Single Index Kit N Set A (10x Genomics, 1000212), respectively. The resulting libraries were sequenced by PE50 sequencing using a DNBSEQ-G400 system (MGI Tech, Shenzhen, China).