scRNA-seq of the patient after regimen adjustment: peripheral blood mononuclear cells
Chromium 5 Library Construction: Fresh cells (300600 viable cells/L) were loaded into the Chromium system to form single-cell GEMs. Reverse transcription was performed on a S1000TM Touch Thermal Cycler (53C for 45 min, then 85C for 5 min), and cDNA was amplified and quality-checked using an Agilent 4200. Libraries were constructed with a Single Cell 5 Library and Gel Bead kit, sequenced with paired-end 150-bp reads on an Illumina NovaSeq6000.