Shotgun metagenomic sequencing of infant gut microbiome sample RB63CA (6 months) from a Pakistani mother-infant cohort
Genomic DNA was extracted from infant stool samples. Short-insert paired-end libraries were prepared by DNA fragmentation, size selection, end repair and A-tailing, adapter ligation, PCR amplification and library QC, followed by DNA nanoball generation and paired-end 150 bp sequencing (PE150) on the DNBSEQ platform (BGI Genomics). Raw reads were quality-filtered with SOAPnuke to obtain clean data for downstream shotgun metagenomic analysis of the human infant gut microbiome.