16S rRNA sequencing of metagenome: dried bamboo scroll
Total genomic DNA was extracted from surface swabs of dried bamboo scrolls and associated storage materials using the DNeasy PowerSoil Pro Kit. The hypervariable V3-V4 region of the bacterial 16S rRNA gene was amplified using the primer pair 341F (5'-CCTACGGGNGGCWGCAG-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3'). PCR products were purified with AMPure XP beads. Sequencing libraries were prepared by adding Illumina adapters and indices, and were sequenced on an Illumina MiSeq platform with a 2x300 bp paired-end strategy.