Constrictotermes cyphergaster gut microbiome under wood diet
A library was constructed, using the Nextera library preparation kit (Illumina), according to the manufacturers instructions. The prepared library was validated using the Agilent bioanalyzer 2100 system with a 12000 DNA assay kit (Agilent) and quantified using Kapa Biosystems next-generation sequencing library qPCR kit (Kapa Biosystems), respectively following the methodology of Moreira et al. (2018). Sequencing was performed at LNBR NGS facility (CNPEM Campinas, So Paulo, Brazil) using an Illumina HiSeq 2500 platform and applying the paired-end protocol (2x100-bp paired ends).