PacBio HiFi seq of ABS4 homozygote
The ABS4 heterozygous line in a HiII background (Dawe, R.K et al. 2023 https://doi.org/10.1038/s41477-023-01370-8, Zhang, H et al. 2012 https://doi.org/10.1111/j.1365-313X.2011.04867.x) was selfed five times, and a sequencing library was prepared using a SMARTbell Express Template Prep Kit 3.0. Sequencing was conducted on a PacBio Revio system with a Revio Sequencing Plate in CCS mode.