Tnseq of adult murine GI tract
DNA was enzymatically fragmented and adapters added using the NEB Ultra II FS kit (New England Biolabs), and ~50ng of fragmented adapted gDNA was used as a template for enrichment by PCR (16 cycles) for the transposon insertions using mariner-specific primer and Illumina P7 primers. Libraries were sequenced on element Aviti platform.