GSM8653934: BRAFV600E patient line LCH1105 replicate 1; Homo sapiens; RNA-Seq
To perform the mRNA sequencing, total RNA was isolated using the RNeasy Tissue kit (Qiagen, Hilden, Germany, cat. 74104) according to the manufacturer's instructions. RNA was assessed for quality using an Agilent Tapestation 4200, and 50 ng of RNA with an RNA Integrity Number (RIN) greater than 8.0 were used to generate RNA sequencing libraries RNA sequencing libraries using the Illumina Stranded mRNA Prep (Illumina) following manufacturer's instructions