Hi-C from Primate iPSC derived Astrocytes (iAstrocytes)
Hi-C from Primate iPSC derived Astrocytes (iAstrocytes)
Cells were detached with Accutase, counted and crosslinked with 1% (final) formaldehyde in their medium. Reaction was quenched with 0.125M (final) glycine, cell pellets were washed with PBS, snap frozen and stored in -80 freezer. Pellet of 10 million cells was used for each CTCF ChIP experiment. iAstrocytes were grown on hESc-qualified Matrigel (Corning)-coated plates, in Astrocyte Medium (ScienCell). Seeding density was 15,000 cells/sq. cm. Hi-C experiments were performed as described as describer before (Rao et al., 2014) Hi-C libraries were generated using NEBNext Ultra II DNA Library Kit, according to manufacturer's instructions