RNAseq of kidney tissue from sham or UUO mice treated with vehicle or EVT0185 (ACLY inhibitor)
RNAseq of kidney tissue from sham or UUO mice treated with vehicle or EVT0185 (ACLY inhibitor)
Mouse kidney cortices were isolated, stored in liquid nitrogen, and processed for RNA extraction. Tissue samples were collected with care to preserve RNA integrity, ensuring high-quality downstream analysis. RNA was extracted from mouse kidney cortices using the RNeasy Kit (Qiagen), following the manufacturerâ€™s standard protocol. This method includes steps for cell lysis, removal of contaminants, and RNA elution to obtain high-purity RNA for sequencing. Poly-A RNA was enriched from total RNA to select for mRNA transcripts. Library preparation, including cDNA synthesis and adapter ligation, was performed by the McMaster Genomics Facility using protocols optimized for the Illumina NextSeq P2 sequencing platform.