RNA-Seq of mouse: stool_6M_sham_No40
DNA was extracted from mouse stool samples using the ISOSPIN Fecal DNA Kit. The V3-V4 region of the 16S rDNA gene was amplified using specific primers. Following amplification, adapter and index sequences were added as part of the 16S Metagenomics Sequencing Library Preparation protocol. The prepared libraries were sequenced using paired-end sequencing on an Illumina platform to assess changes in the gut microbiota.