GSM8653889: M2_CD27+ CD69+; Mus musculus; RNA-Seq
100 cells were sorted directly into SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara) lysis buffer and reverse transcription was performed followed by PCR amplification to generate full-length amplified cDNA Sequencing libraries were constructed using the NexteraXT DNA sample preparation kit with unique dual indexes (Illumina) to generate Illumina-compatible barcoded libraries.