GSM9352933: BMS2; Danio rerio; RNA-Seq
Tissue was homogenized in Trizol and frozen. Then, it was phase separated with chloroform, spun down, and the aqueous phase was extracted. Glycogen and isapropanol was added to the RNA, incubated for 10 minutes, and it was spun down. Supernatant was removed and RNA was washed with 70 percent ethanol. Finally, RNA was allowed to air dry and was suspended in RNAse-free water. RNA libraries for RNA-Seq were prepared using the Kapa mRNA Seq Kit following manufacturer's protocols.