Exome-seq of melanocyte : adult male skin from the right shin
The G&T sequencing protocol was used to separate genomic DNA and mRNA, using a biotinylated oligo d(T)30 VN mRNA capture primer. Genomic DNA was amplified via MDA (Qiagen REPLI-g Single Cell Kit, 150345). mRNA was amplified via SMART-Seq2 (KAPA HiFi HotStart ReadyMix kit, Roche, KK2502).