GSM9237741: RPE1 ATAC-seq rep 1; Homo sapiens; ATAC-seq
ATAC-seq (Assay for Transposase-Accessible Chromatin by Sequencing) was performed according to the Omni-ATAC protocol (Corces et al., 2017). Briefly, 100,000 cells were collected per experiment and nuclei were extracted. Nuclei were incubated with Tn5 on ice for 30 minutes. DNA was purified using the Qiagen MinElute reaction cleanup kit (Qiagen). DNA extracted from ATAC-seq was amplified using the NEBNext high-fidelity PCR master mix (New England Biolabs) and Illumina Nextera DNA indexes (Illumina). Libraries were purified using AMPure XP beads (Beckman Coulter), then profiled using the Agilent TapeStation D1000 high sensitivity ScreenTape on the Agilent 4150 TapeStation System, and sequenced on the Illumina Novaseq X Plus with 150 x 150 bp reads by Novogene (Novogene Corporation Inc. UK).