RNA-seq analysis of HPAEpiCs Cultured at the ALI
RNA-seq analysis of HPAEpiCs Cultured at the ALI
HPAEpiCs were cultured at the ALI for 28 days with medium containing Y-27632, A-83-01, and CHIR99021. HPAEpiCs on ALI day 0, 7, 14, 21, and 28 were washed with PBS and lysed with Lysis buffer in Qiagen RNeasy Mini Kit (Qiagen). Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit (Qiagen). A DNA library was prepared using a TruSeq Stranded mRNA LT Sample Prep Kit (Illumina Inc.).