GSM9427929: Old, long bone, bone marrow; Mus musculus; RNA-Seq
Cells were collected from C57BL6J mice for stromal cell analyses. Hematopoietic cells were depleted using the autoMACS Pro Separator (Miltenyi Biotec). FCM analyses were carried out using the FACS Diva 8.0.1 software (BD Biosciences). Dead cells and debris were excluded using FSC, SSC, and DAPI staining. Cells were sorted using a FACSAria III Cell Sorter (BD Biosciences). Sorted cells were processed with Chromium Next GEM Single Cell 3ʹ GEM kit v.3.1 (10X Genomics) according to the manufacturer's instruction.