16S rRNA sequencing of partial denitrification-anammox granular sludge microbial community
Genomic DNA was extracted from partial denitrification-anammox (PD/A) granular sludge samples. The V3-V4 hypervariable region of the 16S rRNA gene was amplified by PCR using universal primers. Amplicon libraries were constructed and sequenced on the Illumina MiSeq platform with paired-end reads.