GSM9465660: 2.5_3h_WT_for_IntS11_KD_PolII_ChIPseq_replicate_1; Drosophila melanogaster; ChIP-Seq
The embryos of indicated genotypes were harvested and subjected to cross-linking by fixation buffer (50 mM HEPES, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 100 mM NaCl and 1.8% formaldehyde).  They were cross-linked for 20 minutes and then quenched with 125 mM glycine (final concentration) for 5 minutes at room temperature.  Chromatin immunoprecipitation (ChIP) reactions were carried out in duplicate using the Pierce Magnetic ChIP Kit. Sequencing libraries were generated using the VAHTS Universal DNA Library Prep Kit for MGI.