whole genome sequencing of Pseudomonas aeruginosa PAO1: Co-evolved with Luz19 bacteriophage
Genomic DNA was prepared for whole genome sequencing using the Illumina DNA Prep tagmentation kit with unique dual indexes for sample multiplexing. Sequencing was conducted on an Illumina NextSeq2000 platform (2x150 bp paired-end reads) with a 1-2% PhiX spike-in for optimal base calling. Reads were demultiplexed, trimmed, and analyzed using DRAGEN v4.2.7, and quality checked with FastQC. FASTQ files follow a naming convention for sample tracking. If coverage was insufficient, samples were re-sequenced.