GSM8994035: WT_C_2; Mus musculus; RNA-Seq
High-throughput mRNA sequencing was performed by the LC-Bio Technology co.ltd., (HangZhou, China). The quality of total RNAs was assessed by BioAnalyzer 2100 (Agilent Technologies). RNA libraries were constructed with TruSeq Stranded Total RNA Library Prep kit (Illumina). Libraries were quality-controlled for size distribution and quantified with Qubit Fluorometer (Thermo Fisher Scientific). The libraries were sequenced on an Illumina HiSeq sequencer for 150-bp paired-end reads according to the manufacturer's instructions.