RNAseq of MM0196 grown on lactose
The RNA was purified using the RNAesy Mini Kit (Qiagen, UK), following the manufacturers protocol. RNA concentration and purity were evaluated by a Picodrop microliter spectrophotometer (Picodrop, UK). Before RNA sequencing, 2.5 g of total RNA was treated to remove the ribosomal RNA by the Ribo-Zero Magnetic Kit (Illumina), followed by purification of the rRNA-depleted sample by ethanol precipitation. RNA was processed according to the manufacturers protocol. The yield of rRNA depletion was confirmed by a Tape station 2200 (Agilent Technologies, USA). A whole transcriptome library was constructed using the TruSeq Stranded RNA LT Kit (Illumina) and samples were loaded into a NextSeq High Output v2 Kit Chemicals 150 cycles (Illumina), according to the technical support guidelines