GSM9570620: Hep3B-Con-B1; Homo sapiens; RNA-Seq
Trizol reagent is a reagent used to directly extract total RNA from cells or tissues. It can maintain the integrity of RNA while lysing and dissolving cells. After lysing the cells and releasing RNA, adding chloroform and centrifuging will separate the sample into an aqueous layer and an organic layer. Under acidic conditions, RNA can be separated from DNA, and RNA is present in the aqueous layer. After collecting the above aqueous layer, RNA can be recovered by isopropanol precipitation. The extracted mRNA is enriched using mRNA Capture Beads. After purification with beads, the  mRNA is fragmented using high temperatures. The fragmented mRNA is then used as a template  to synthesize the first strand of cDNA in a reverse transcription enzyme mixture system. While  synthesizing the second strand of cDNA, end repair and A tailing are completed. Next, adapters are ligated, and Hieff NGS® DNA Selection Beads are used for purification to select target  fragments. PCR library amplification is then performed, and finally, detection is carried out using  the Illumina Novaseq X Plus.