Small RNA sequencing of Dicer-digested db-siRNA
Purified db-siRNA was digested with recombinant human Dicer at 37 degrees C for 12 h. Cleavage products were separated by 15% PAGE, and the recovered small RNA products were ligated to 3-prime and 5-prime adapters, reverse transcribed, PCR amplified, and sequenced as single-end reads on an Illumina platform.