Whole genome sequencing of sample collected from patient MEA253 at complete remission timepoint.
PCR-free WGS libraries were prepared in-house from 100-150 ng of high-quality genomic DNA from fresh or flash-frozen samples using the NEBNext Ultra II FS DNA PCR-Free Library Prep Kit for Illumina (E7430L). Enzymatic fragmentation was performed for 8 minutes to achieve a target average insert size of 450 bp. NEBNext Multiplex Oligos for Illumina Unique Dual Index UMI Adaptors DNA Set 1 (E7395S) were used for library preparation. Library concentration was measured by qPCR using KAPA Library Quantification Kits (KK4824) on a QuantStudio 5 instrument, and fragment size was estimated from fragmentation conditions.