GSM8827041: Female BALB/c naïve mouse stomach 3; Mus musculus; RNA-Seq
Stomach tissues from mice post multiple infections were collected 4 days p.i. into 1mL of 1x DNA/RNA Shield reagent (R1100-50, Zymo Research, Irvine, CA) in a 2mL ZR bashingbead lysis tube (S6012-50, Zymo Research, Irvine, CA). The tissues were lysed using a Precellys 24 homogenizer (03119.200.RD000, Bertin Technologies, Montigny-le-Bretonneux, France) using the 6m/sec setting for 40s. The homogenates were transferred to 1.5mL DNA LoBind tubes (022431021, Eppendorf, Hamburg, Germany) and shipped on dry ice to SeqCenter (Pittsburgh, PA) for RNA extraction, polyA purification, and RNA sequencing. RNA was extracted using the Quick-RNA miniprep kit (R1054, Zymo Research, Irvine, CA). Library preparation was carried out by SeqCenter (Pittsburgh, PA) using the Illumina Stranded Total RNA Prep ligation with Ribo-Zero Plus kit and 10bp unique dula indices. Sequencing was performed on a NovaSeq X Plus for 150bp paired-end reads.