Bulk RNA-Seq of Drosophila melanogaster heads after neuronal Wwox knockdown and AB42 overexpression versus control
Bulk RNA-Seq of Drosophila melanogaster heads after neuronal Wwox knockdown and AB42 overexpression versus control
10 heads from 14 day old Drosophila melanogaster were dissected and pooled per replicate, per genotype. Tissue was snap frozen prior to extraction. Pooled heads were manually homogenised and RNA was subsequently extracted using the RNAqueous-Micro kit (AM1931) as per the manufacturer's instructions. The Cardiff University Genome Hub performed Qubit and Tapestation quality/quantity controls prior to library preparation. Samples were then normalised and library prep was performed. The latter was achieved following the Illumina TruSeq stranded mRNA kit. Here mRNA was enriched by poly-A selection and Truseq3 adapters added.