GSM8967719: OVCAR4, shRNA#2-MPZL3, replicate 1; Homo sapiens; RNA-Seq
Total RNA was extracted from cells using Direct-zol RNA Miniprep kit (Zymo Research, R2052). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by second strand cDNA synthesis using dTTP, end repair, A-tailing, adapter ligation, size selection, amplification, and purification for non-directional library preparation. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection.