GSM8574902: Breast_Cancer_cfDNA_5; Homo sapiens; ATAC-seq
NEXTFLEX Rapid DNA-Seq Kit 2.0 (Approximately  650µL of blood plasma from cancer patients or healthy donors was used for cfDNA purification utilizing the SubXTM-Exo-DNA kit (Capital Biosciences). The purified cfDNA was then quantified using the Qubit dsDNA HS Assay kit. Libraries were prepared using the NEXTFLEX Rapid DNA-Seq Kit 2.0 (Revvity). Half the volume of purified cfDNAs, ranging from approximately 1 ng to 10 ng, was utilized. After adapter ligation, a total of 11 PCR cycles were performed.