Raw Oxford Nanopore Technology genome sequence of Naja sumatrana
Sequencing libraries were prepared using the Ligation Sequencing Kit V14 (SQK-LSK114) with minor protocol adjustments. A total of 1,500 ng of input DNA was used for library preparation in 48 L nuclease-free water. The end-preparation reaction was incubated at 20 C for 30 min followed by 65 C for 5 min. During adapter ligation, the adapter-bound DNA was spun down and incubated at 37 C for 30 min using a heat block, with gentle mixing by flicking every 5 min to improve ligation efficiency.