GSM9679933: Vehicle-treated I/R mouse brain, replicate 1; Mus musculus; RNA-Seq
Total RNA was isolated from ischemic hemispheres using Magzol Reagent (Magen) following the manufacturer's instructions. RNA quantity and quality were assessed using K5500 spectrophotometer (Kaiao) and Agilent 2200 TapeStation. Poly(A)+ mRNA was enriched using NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB), fragmented to ~200 bp, and processed with NEBNext® Ultra™ RNA Library Prep Kit for Illumina. Libraries were validated using Agilent 2200 TapeStation and Qubit (Thermo Fisher).