GSM9352628: Hgc27, SG12, LPDS, biol rep3; Homo sapiens; RNA-Seq
Total RNA was isolated using Qiagen Rneasy mini kit. Isolated RNA samples were quality checked, and RNA integrity numbers (RIN) were assessed with an RNA ScreenTape assay kit on the 4200 TapeStation system (Agilent Technologies). Quantification of RNA samples was determined with the Qubit RNA Broad Range Assay Kit using Qubit Flex Fluorometer (Invitrogen). 100 ng of total RNA input was subjected to library construction with the Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus kit (Illumina).