GSM9139430: snATAC-Seq - 1224_3_rep2; Homo sapiens; ATAC-seq
We enzymatically digested and dissociated fresh tumor samples according to tissue dissociation kit protocols using the gentleMACS™ Dissociator (Miltenyi). Dissociated tissues were filtered, centrifuged, and the isolated cells were resuspended in 0.1% BSA in PBS and immediately processed for the generation of snATAC-seq libraries. We performed snATAC-seq using the Chromium single cell ATAC v1 chemistry from 10x Genomics with the target of 5000 nuclei per sample. Then the libraries were profiled using the Novaseq S2 (Illumina) technology.