Act16G
The SMRTbell library was constructed using the SMRTbell Template Prep Kit 2.0. Genomic DNA was sheared to an appropriate size using a Covaris g-TUBE, followed by damage repair and end-repair. Hairpin adapters were ligated to the DNA fragments, which were then purified and size-selected using AMPure PB beads and the Blue Pippin System. The library was further purified, quantified with Qubit, and assessed for insert size using the Agilent 2100 Bioanalyzer. Sequencing was performed on the PacBio platform.