GSM9023322: OT1 CD8+ Tcells, repeat stimulation, d8, replicate 2; Mus musculus; RNA-Seq
Total RNA was extracted using a total RNA isolation kit (Vazyme). 1 μg of total RNA was used for the construction of sequencing libraries. For library preparation, 1 µg of total RNA was used with the Hieff NGS® Ultima Dual-mode mRNA Library Prep Kit (YEASEN) following manufacturer's protocol.