DNA panel sequencing and bulk RNA sequencing of established sarcoma 3D models in the MASTER precision oncology registry
For targeted sequencing, 120 ng of total DNA was utilized. Library preparation followed the TruSight Oncology 500 Panel Kit (Illumina, San Diego, CA, USA) protocol 1,2. The barcoded libraries were sequenced (2  150 bp paired-end) on an Illumina NextSeq500 platform.