Arabidopsis thaliana, atcfi59-1, ONT DRS, SQK-RNA002, biological replicate 1
 Total RNA was extracted from 60 mg of plant material from pooled seedlings ground in liquid nitrogen using the RNeasy Plant Mini Kit (Qiagen) according to the manufacturer's instructions. The mRNA enrichment was performed using the mutated recombinant initiation factor GST-eIF4EK119A according to Bilska et al. 2020.  For each biological replicate, 2–5 ug of cap-enriched plant RNA was mixed with 100–150 ng of Saccharomyces cerevisiae oligo(dT)-enriched RNA, 2–2.5 ug of murine cap-enriched RNA, and 50 ng of in vitro-transcribed poly(A) standards. Libraries for nanopore DRS were prepared using SQK-RNA002 sequencing kit (ONT) following the manufacturer's protocol. Sequencing was performed on the R9 flow cell with the MinION (ONT) device controlled by MinKNOW (ONT) software.       