GSM8827058: testes of blue catfsh sampled at age 7, biological rep3; Ictalurus furcatus; RNA-Seq
Total RNA was extracted using Quick-RNA Miniprep Plus Kit (Zymo Research) following the manufacturer's instructions. Total RNA was treated with DNase I and resuspended in 50 µL of nuclease-free water. Sequencing libraries (n = 16) were prepared using a NEBNext Ultra II RNA Library Preparation Kit (NEB, Ipswich, MA, U.S.), following manufacturer's instructions