Genome-wide DNA methylation profiling of regenerating ear pinnae in BALB/c mice induced with pharmacological epigenetic therapy involving subcutaneous administration of zebularine and retinoic acid in alginate hydrogel
Genome-wide DNA methylation profiling of regenerating ear pinnae in BALB/c mice induced with pharmacological epigenetic therapy involving subcutaneous administration of zebularine and retinoic acid in alginate hydrogel
The tissues were collected 7 days after the injury, placed immediately in liquid nitrogen and stored at -80Â°C. Genomic DNA was extracted from 3 mm rings surrounding the initial punch wounds using DNeasy Blood and Tissue kit (Qiagen.) Extracted DNA was quantified spectrophotometrically on the Varioskan Lux (ThermoFisher Scientific), and DIN (DNA integrity number) was determined with Genomic DNA Screen Tape Analysis on 4510 TapeStation System (Agilent). All samples with DIN >7.0 were further processed to prepare RRBS library.