Sample 2
DNA Source: Bacterial broth cultureExtraction: High-molecular-weight (HMW) DNA isolated using ZymoBIOMICS HMW MagBead DNA Kit (following manufacturers protocol).Library Prep:Kit: Oxford Nanopore Rapid Barcoding Sequencing Kit (SQK-RBK114.24)Input: 50 ng HMW DNA tagmented via transposase (no end-prep/ligation).Cleanup: AMPure XP beads (1:1 ratio).Sequencing:Flow Cell: R10.4.1 (FLO-MIN106)Device: MinION Mk1BRun Time: 6 hours (active channel selection enabled)Basecalling: Dorado V1 (min Q-score: 7)