Biomolecular Condensate Coronas from Nanoparticle Intracellular Processing Are Transferred Between Cells Allowing Cytosolic and Nuclear Access
Biomolecular Condensate Coronas from Nanoparticle Intracellular Processing Are Transferred Between Cells Allowing Cytosolic and Nuclear Access
Cells were exposed to nanoparticle suspension for 10 min (pulse). The suspension was removed. Cells then were incubated complete medium containing FBS for 4 h (chase). After the chase, the supernatants were collected and clarified to remove cell debris. The exported particle complexes were pelleted by centrifugation at 12,000 x g for 40 min at 4 Â°C.  A549 cells were cultured in normal growth media. The cells were treated with nanoparticles for 10 min pulse followed by 4 hour chase. The conditioned media were collected. We used QIAGEN miRNeasy Micro Kit (Cat. No. 217084) to extract RNA. MGIEasy RNA Library Prep Set