Bulk RNAseq of Rhesus PFC Exposed to THC Prenatally
RNA extracted from frozen lung samples was sent to Novogene for RNA QC, library preparation, and sequencing.  Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis, A-tailing, adapter ligation, size selection, amplification, and purification. Libraries were quantified with Qubit and real-time PCR and assessed for size distribution with a bioanalyzer. Quantified libraries were pooled and sequenced on an Illumina NovaSeq 6000 with 30M (150bp paired-end) reads per sample.