WGS of Cryptococcus neoformans: Strain MCM096-A
DNA was extracted with MasterPure Yeast DNA Purification kit and cleaned up with the Zymo Research Genomic Clean and Concentrator kit (following manufacturer's instructions) followed by quantification with PicoGreen. After quantifying the DNA with PicoGreen, samples were prepped for genomic sequencing using seqWell's plexWell 96 kit to prepare the libraries using the manufacturer's protocol. Briefly, samples were individually bar-coded in sets of 96, pooled and purified. Each pooled sample was bar-coded, enriched, and finally size-selection purified. Libraries were sequenced at Duke University's Sequencing and Genomic Technologies Facility on the NovaSeq 6000 S-Prime with 150 bp paired-end reads.