Mouse fecal metagenome for sample 505 week 3
Libraries were prepared by fragmenting 500 ng of genomic DNA from each sample by sonicating with Adaptive Focused Acoustics (E220 Focused Ultrasonicator, Covaris, Woburn, Massachusetts). This produced an average fragment size of 500 base pairs. The KAPA Hyper Prep Kit (KAPA Biosystems, Wilmington, MA, USA) was used to generate sequencing libraries following manufacturers instructions using 4 cycles of amplification.