GSM9350625: Neutrophils from mice fed ad libitum, replicate #3; Mus musculus; RNA-Seq
Micro RNA Isolation Kit (Qiagen), according to the manufacturer's instructions. For naïve and effector CD8+ T cells, cDNA was synthesized with the Ultralow V4 kit (Clonetech) and sequencing libraries were prepared with the Nextera XT DNA prep kit (Illumina). For neutrophils, cDNA libraries was synthesized with the NEB Ultra II Directional RNA Library Prep (plus Poly A isolation module) and sequencing libraries were prepared with the Illumina NovaSeq X Plus kit.