RNA-seq of Sus scrofa:14d male testis
1. Through the oligo - dT beads capture with RNA polyA tail; 2. Heating, to capture snippets of mRNA for 200 bp - 300 bp; 3. Add the strand short master mix of incubation, mRNA transcribed into cDNA.4. End repair of DNA fragment, adding A base at the 3 'end, connecting sequencing connector; 5. PCR amplification and fragment size selection of DNA products (generally 300-400bp, including adaptor sequences).The library was sequenced by illumina NovaSeq 6000 platform