16s metagenome of Mus musculus: Cecum Female 9wk
Bacterial genomic DNA was extracted from mouse fecal samples using the Nucleospin DNA stool Kit according to the manufacturers instructions. The V3V4 region of the 16S rRNA gene was amplified by PCR using primers 341F and 805R. Sequencing libraries were prepared following the Illumina 16S Metagenomic Sequencing Library Preparation protocol with dual-index barcodes. Libraries were sequenced on an Illumina MiSeq platform with 2  300 bp paired-end reads.