Nanopore whole genome sequencing of ESBL-producing E.coli (ESBLEC) MG12
Genomic DNA was prepared for sequencing using a ligation-based library preparation with native barcoding, employing the Oxford Nanopore Technologies Native Barcoding Expansion kit (EXP-NBD114) to enable multiplexing of multiple samples. Sequencing was done on a Minion R10.4.1 flowcell. Basecalling was performed using Dorado with the super accuracy (SUP) model (dna_r10.4.1_e8.2_400bps_sup)