GSM9020512: 58187_549WO; Homo sapiens; RNA-Seq
RNA extraction, sequencing, and analysis RNA from 100,000 cells from each CAR T cell product was extracted with the miRNeasy Mini Kit (QIAGEN, 217004), and RNA quality was assessed using the Agilent 2100 Bioanalyzer according to the manufacturer's instructions. The libraries were prepared with SMART-Seq® Ultra Low Input RNA Kit for Sequencing – v4 (TaKaRa, Cat. 634888) according to the manufacturer's protocol. The final libraries were validated with the Agilent Bioanalyzer DNA High Sensitivity Kit and quantified with Qubit. Sequencing was performed on Illumina HiSeq 2500 with the single read mode of 51cycle.