GSM8573488: GFP+ P14 CD8 T cells, CRIPSR, rep_4 of of LCMV Cl13 SPL; Mus musculus; OTHER
Cas9 transgenic P14 cells were transduced with gRNA and transferred in LCMV Armstrong or Clone 13 strain infected animal (day 1). On day 18+ of post-infection, T cells were harvested from spleen and/or small intestine. GFP positive P14 CD8 T cells were FACS sorted and single cells were encapsulated in gel-beads-in emulson (GEMs) uising the 10X Controller with a Chromium Single Cell 5' GEM Libarary and Gel Bead kit. Chromium GEM-X Single Cell 5' Reagent Kits v3 with Feature Barcode technology for CRISPR Screening & Cell Surface Protein