Nkx3.1-CreERT2/+ Pten-L/L Smad4-L/L Pygo2-L/L single-cell, castrated, Sx682, ICB, replicate 1, hashtag TotalSeq-A0307
Total RNA isolated using Direct-zol RNA MiniPrep Kit (Zymo R2050). For scRNA-seq: tumors dissociated with Mouse Tumor Dissociation Kit (Miltenyi 130-096-730), 1-2h 37C, filtered through 40-m strainer, dead cell depleted with EasySep Annexin V kit (STEMCELL 17899), TotalSeq-A hashtag A0303-A0308 applied.