RNAseq of NOD pups: GI tissue
mRNA was purified from total RNA, cDNA synthesized using random hexamers, followed by end repairing and A-tailing, adapter ligation, size selection, PCR amplification, and purification. cDNA libraries were quantified and analyzed for size distribution. Following sequencing, raw data underwent quality control, including examination of error rate distribution, GC-content distribution and data filtering (removal of reads with adaptor contamination, N>10%, and >50% low quality base calls).