16S rRNA gene amplicon sequencing of ileal microbiota from broiler chicken PC5
Total microbial DNA was extracted from broiler chicken ileal contents using the QIAamp Fast DNA Stool Mini Kit. The V3-V4 region of the bacterial 16S rRNA gene was amplified using primers F341 and R806. PCR products were purified and quantified, and the resulting amplicon libraries were sequenced on an Illumina MiSeq platform using paired-end 250-bp sequencing.