GSM8459505: LPS_MMF_4h_2; Mus musculus; RNA-Seq
RNA was extracted using the Maxwell® 16 LEV simply RNA Cells kit and the robotic platform Maxwell® 16 (both from Promega Biotech Ibérica), following the manufacturer's instructions. Poly(A)+ mRNA fraction was isolated from total RNA and cDNA libraries were obtained following Illumina's recommendations. Briefly, poly(A)+ RNA was isolated on poly-T oligo-attached magnetic beads and chemically fragmented prior to reverse transcription and cDNA generation. The cDNA went through an end repair process, addition of a single “A” base to the 3' end and ligation to the adapters. Finally, the products were purified and enriched with PCR to create the indexed final double stranded DNA library. The quality and quantity of the libraries were analysed in tapeStation 4200, using the High Sensitivity assay (Agilent Technologies)