DNA-seqs of Actinopterygii: Escambia River System
DNA extraction was performed with the DNeasy Blood & Tissue kit (Qiagen) on pooled pellets from water samples. Extracted DNA samples were amplified with Polymerase Chain Reaction (PCR). Universal MiFish primers were used in PCR to target and amplify the mitochondrial 12S rRNA gene of ray-finned fishes (Actinopterygii). Duplicates of PCR products per sample were merged and quantified using a Qubit dsDNA BR Assay kit. Libraries were normalized using the SequalPrep Normalization Plate Kit. Libraries were quantified using qPCR and the KAPA Illumina Library Quantification Kit on a LightCycler Real-Time PCR according to manufacturer guidelines. Libraries were sequenced at the Hubbard Center for Genomics, Sequencing Core Facility (Durham, NH) and run on an Illumina HiSeq platform.