"PANC1 cells exposed to pH 7.4 for 24 h, floating fraction, rep3_R1"
Total RNA was extracted from cultured cells using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. RNA quality was assessed prior to library preparation. Poly(A) RNA was enriched using the NEBNext Poly(A) mRNA Magnetic Isolation Module (Cat No. E7490). Strand-specific libraries were constructed using the NEBNext Ultra II Directional RNA Library Prep Kit (Cat No. E7760) according to the manufacturer's protocol. Sequencing was performed on an Illumina NovaSeq 6000 platform to generate 150 bp paired-end reads (PE150). Approximately 4 Gb of data were generated per sample, corresponding to about 26.7 million reads (13.3 million read pairs) per sample.