GSM8654480: MCF7, KPNA2-BD antibody, ChIP; Homo sapiens; ChIP-Seq
Cells were fixed with formaldehyde solution in the culture mediumfor 5 min. After cell lysis, chromatin was sonicated using a Branson 250 Sonifier (Branson Ultrasonics). ChIP library was prepared using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs).