Transcriptome comparisons of dimorphic Arthrostemma ciliatum stamen whorls at different developmental stages to find candidate genes causing heteranthery
Transcriptome comparisons of dimorphic Arthrostemma ciliatum stamen whorls at different developmental stages to find candidate genes causing heteranthery
Multiple flower buds of a defined size (corresponding to the developmental stage) were collected from plants grown in the greenhouse.  Multiple flower buds corresponding to each developmental stage were dissected under a stereo microscope and the two stamen whorls (outer-antesepalous, inner-antepetalous) were separated into pre chilled, labelled Eppendorf tubes (kept in a metal rack on dry ice) for extraction.  Plants were grown in the public greenhouses at the Botanical Garden Munich Nymphenburg RNA was extracted from each stamen whorl following the manufacturers protocol using the RNeasy Plant Mini Kit (Qiagen) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. 