GSM9644152: HUES64, Nocodazole; Homo sapiens; OTHER
Genomic DNA was isolated using the Puregene Cell Kit (Qiagen, 158043) according to the manufacturer's instructions. For samples labeled with 500 µM BrdU and samples not labeled with BrdU, libraries were prepared using a Ligation Sequencing Kit (Oxford Nanopore Technologies, SQK-LSK114) according to the manufacturer's instructions. Libraries generated from labeled samples were loaded onto R10.4.1 flow cells (Oxford Nanopore Technologies, FLO-MIN114) and sequenced using a MinION Mk1b device. Libraries for samples labeled with BrdU and samples not labeled with BrdU were loaded onto R10.4.1 flow cells (Oxford Nanopore Technologies, FLO-PRO114M) and sequenced on a PromethION P2 solo device (Oxford Nanopore Technologies) for 72 hours. Minimum read length was set to 1000 bp. All sequencing data was collected using MinKNOW v23.07.5.