RNA-Seq of H.sapiens PBMC
performed by RNA-Seq (Illumina, paired end reads, 150bp). Quality control of the generated reads, mapping, and selection of genes with significant differential expression was carried out using CLC Genomics Workbench package 22 (QIAGEN) (20), HiSAT2 (21), and edgeR (22). Human genome 38 and its annotations (GRCh38) were downloaded from Ensembl genome browser (https://www.ensembl.org/index.html) and used for mapping and expression profiling. Mapping was carried out with the following parameters: mismatch cost of 2, insertion cost of 3, deletion cost of 3, minimum length fraction of 0.8, and minimum similarity fraction = 0.8.