Bulk_S (CRX2037928; CRA030777)
To conduct bulk segregant analysis, 30 plants exhibiting a wild-type phenotype and 30 displaying the mutant phenotype were selected from the F2 population derived from the cross between sms6 and Zp661. Genomic DNA was extracted from all individuals and mixed in equal amounts to construct the wild-type (Bulk_L) and mutant (Bulk_S) DNA pools.