Whole genome sequencing of sample collected from patient MEA083 at time of diagnosis timepoint.
PCR-free WGS libraries were prepared in-house from 300 ng of input genomic DNA using the Illumina DNA PCR-Free Prep, Tagmentation kit (part number 20041795) following the manufacturer's default protocol. IDT for Illumina DNA/RNA UD Indexes Set A dual-index adapters were used to prevent index hopping. Library concentration was measured using the Qubit ssDNA Assay Kit. Libraries were sequenced on Illumina NovaSeq 6000, targeting 120X coverage.