GSM9921392: SPExp Brain 2; Mus musculus; RNA-Seq
Whole brains were dissociated, nuclei were isolated using the 10x Genomics Nuclei Isolation Kit with RNase Inhibitor according to the manufacturer's protocol, and nuclei were counted and quality assessed prior to library preparation. Single-nucleus RNA-seq libraries were prepared using the 10x Genomics Chromium GEM-X Single Cell 3′ Gene Expression v4 platform according to the manufacturer's instructions. Libraries were sequenced on an Illumina NovaSeq X instrument.