GSM9352580: glp-1, Naïve, timepoint2, rep4; Caenorhabditis elegans; RNA-Seq
Extraction protocol: RNA was extracted using TRIzol-chloroform and treated with TURBO DNase(Ambion), followed by purification using an RNA Clean-up Kit (Zymo). For each sample, 70-100 ng of total RNA was used to construct sequencing libraries. Further details are provided in the Materials & Methods section RNA-seq libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit (Lexogen).