GSM9822966: tumor cellr, Low glucose MYC overexpression, replicate 1; Homo sapiens; OTHER
Cells were collected and incubated with concanavalin A–coated magnetic beads. Cell membranes were permeabilized, and bead-bound cells were incubated with an anti-MYC primary antibody overnight at 4°C, followed by incubation with a secondary antibody. After washing, the cells were incubated with a protein A–Tn5 transposase fusion complex for antibody-guided chromatin tagmentation. CUT&Tag libraries were constructed using the Hyperactive Universal CUT&Tag Assay Kit (Vazyme Biotech, TD904) according to the manufacturer's protocol. Tagmented DNA was purified and PCR-amplified to generate sequencing libraries. Libraries were subjected to paired-end high-throughput sequencing on the SURFSeq 5000 platform.