GSM9429383: SAT, rep2; Oncorhynchus mykiss; RNA-Seq
Adipose tissue was dissected from fish (visceral, subcutaneous, and intramuscular depots). Mature adipocytes were isolated by mechanical and enzymatic digestion using collagenase type II. Total RNA was extracted using TRIzol reagent (Thermo Fisher Scientific, Waltham, MA, USA), followed by chloroform, isopropanol, and ethanol extraction according to the manufacturer's instructions. RNA quality and integrity were assessed prior to sequencing. RNA sequencing libraries were prepared by Azenta/GENEWIZ (Leipzig, Germany). Ribosomal RNA was depleted prior to library construction. Separate libraries were generated for mRNA-seq and miRNA-seq following standard Illumina-compatible protocols. Libraries were sequenced on an Illumina NovaSeq platform using a 2 × 150 bp paired-end configuration for mRNA libraries.