WGS of F1 F 5th instar larva
Genomic DNA was extracted using the Quick-DNA Microprep Kit (Zymo Research) following manufacturers protocol with slight modification, i.e. digested with Genomic Lysis Buffer (with addition of beta-mercaptoethanol). DNA was quantified using Nanodrop and checked with 1% agarose gel. DNA samples with purity A260/A280 > 1.8 were used for generating Illumina libraries using DNA Prep Kit (Illumina) according to the manufacturers protocol, with 5 cycles of PCR amplification using Nextera-Compatible Multiplex Primers (Active Motive). QC of WGS libraries was performed using Qubit and TapeStation