GSM8653708: colorectal tumors, cKO, scRNAseq, rep2; Mus musculus; RNA-Seq
Tumors were minced thoroughly and digested by using mouse tumor dissociation kit as the manufacturer's recommendations. Dead cells were stained with fixable viability kit and sorted to remove. scRNA-seq libraries were generated with the use of the Chromium Single Cell 30 Reagent Kit v2 (10X Genomics). 2,000 cells/μL tumor-dissociated cells were loaded onto the 10X Chromium Single Cell Platform (10X Genomics). Generation of gel beads in emulsion (GEMs), barcoding, GEM-RT clean-up, complementary DNA amplification and library construction were all performed according to the manufacturer's protocol.