GSM8827989: MEF_PUS10KO_tdR_SCR_n2; Mus musculus; RNA-Seq
For the RNA sequencing upon transfection with the tdRs, PUS10 KO MEFs were plated in gelatin-coated 10cm plates at a density of 1 × 106 cells and transfected with 20 nM of tdR-5-GlyGCC or tdR-SCR. 24h post transfection, cells were harvested and lysed in TRIzol. RNA was isolated using the Quick-RNA Miniprep Kit (Zymo Research). Sequencing libraries were generated using 500 ng of total RNA with the TruSeq Stranded Total RNA Library Prep Kit (Illumina) according to the manufacturers' instructions. Libraries were sequenced on an Illumina NovaSeq 6000 with paired end 150 cycles.