GSM9237709: LP09018; Homo sapiens; RNA-Seq
Total RNA was extracted using the GeneMATRIX Universal RNA Purification Kit (EURX, Gdańsk, Poland) with rDNase set (Macherer-Nagel, Düren, Germany ). Sequencing libraries from total RNA were prepared at the Core Facility Genomics, Medical University of Vienna, using the QuantSeq 3' FWD protocol version 2 with unique dual indices (Lexogen GmbH, Vienna, Austria). Seventeen PCR cycles were used for library preparation, as determined by quantitative PCR (qPCR) according to the library preparation manual.