GSM8967856: WGBS Pld6+/+ wild-type; Mus musculus; Bisulfite-Seq
DNA was extracted by phenol-chloroform extractio. Genomic DNA was fragmented by sonication on Bioruptor for 15 cycles of high output for 30 seconds, then purified by Agencourt AMPure XP. After the bisulfite treatment as described previously (Ichiyanagi et al. 2011), the sequencing library was made using ACCEL-NGS methyl-seq library Kit (Swift Biosciences).