GSM8452803: fpECs early-onset preeclampsia 1; Homo sapiens; RNA-Seq
1x10^6 fpECs per sample were harvested using TrypLE and stored in QIAzol Lysis Reagent. RNA was isolated using the miRNeasy Micro Kit (Qiagen) and quality was assessed using a 2100 Bioanalyzer (Agilent, Santa Clara, USA). A NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) was used to extract mRNA from total RNA followed by library preparation with a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs).