Whole genome sequencing of sample collected from patient MEA168 at time of diagnosis timepoint.
PCR-free whole genome sequencing (WGS) libraries were prepared by Broad Clinical Laboratories from 275-350 ng of high-quality genomic DNA extracted from fresh or flash-frozen bone marrow and blood samples, using the Kapa Biosciences HyperPrep library construction kit (KK8515). Custom Broad dual-index adapters were used to prevent index hopping during sequencing. Completed libraries were sequenced on the Illumina NovaSeq X Plus system, targeting 60X coverage.