RNA-Seq of K562 WT control
K562 WT and HRG1-KO cells were grown on T75 flasks with RPMI supplemented with 10% HI-FBS, 1% Penicilline-Streptamycine-Glutamine, and 1% non-essential amino-acids. Cells were not treated (control) or treated with 0.75 mM sodium butyrate 24h after being plated, and treated a second time 48h after the first treatment. Cells were collected 6h after the second treatment and washed three times with 1X DPBS. Total RNA was extracted using TRIzol lysis reagent (Invitrogen, #15596018). A total of 12 samples were sequenced using Illumina's Novaseq platform (Novogene), with triplicate of each genotype. Paired-end 150 base reads were sequenced and checked by FastQC, version 0.12.1.