GSM8604990: Macrophages, BAL, Sig F KO Naïve, mouse #1, run 1; Mus musculus; RNA-Seq
Total RNA was extracted using RNeasy Micro Plus Kit from Qiagen per manufacturer's instructions. The Universal Plus mRNA-Seq library preparation kit with NuQuant (Tecan, Männedorf, Switzerland) was used  with an input of 200 ng of total RNA to generate RNA-Seq libraries. Paired-end sequencing reads of 150 bp was generated on NovaSeq 6000 (Illumina, La Jolla, CA) sequencer at a target depth of 80 million paired-end reads per sample.