RNA-Seq of Sus scrofa: lung
In normal species, over 90% of the RNA is rRNA. Therefore, after extracting the total RNA from the sample, we need to remove rRNA using a conventional kit and enrich mRNA. We then reverse transcribe the enriched mRNA to form double-stranded cDNA. After repairing the double ends of the cDNA, we add adapters and perform PCR amplification to construct the sequencing library for submission.