GSM8655213: A549 cells, ChIP-seq_anti-flag(ZNF296); Homo sapiens; ChIP-Seq
3 × 10⁷ cells were cross-linked using 1% formaldehyde and quenched with 0.125 M glycine. Lysates were prepared in buffer (50 mM HEPES-KOH pH 7.5, 1 mM EDTA, 0.1% SDS, 140 mM NaCl, 0.1% sodium deoxycholate, and 1% Triton X-100), sonicated on ice, and precleared. Chromatin-immunoprecipitated (ChIP) DNA was purified after elution and reverse cross-linking, followed by library preparation using the Universal Pro DNA Library Prep Kit for Illumina (Vazyme ND608)