GSM9680082: HTO counts of multiplex sSL0238 containing tissue & timepoint(s) CTX (L); P4; Mus musculus; OTHER
Single nuclei were extracted using the 10X Genomics nuclei isolation kit (Chromium Nuclei Isolation Kit CG000505, Rev A). Samples were pooled after barcoding with cholesterol-modified oligos (CMOs) and a CMO Co-Anchor. Afterwards, single nuclei of pooled samples were encapsulated in Gel-Beads-in  emulsions (GEMs) on a 10X Chromium Controller using Chromium Next GEM Chip J Single Cell kit (PN-1000230), Chromium Next GEM Single Cell Multiome GEM Kit A (PN-1000232), Chromium Next GEM Single Cell Multiome Gel Bead Kit A (PN-1000231) as instructed by the manufacturer. The library was prepared according to the 10X protocol using Chromium Next GEM Single Cell Multiome ATAC + Gene Expression, CG000338 Rev F. The hasthag oligo (HTO) libraries were prepared alongside cDNA amplification. Here, an ADT additive primer was added to the reaction. During cDNA cleanup, supernatant was saved to purify Hash-DNA. HTO libraries were generated using unique combinations of i5 and i7 indices.