GSM8655031: Input3_MLTC-1_6, IgG; Mus musculus; OTHER
Twenty ug of RNA with a RIN of 8.35 was used for immunoprecipitation using the validated Abcam ab70775 antibody. As a negative control, in parallel, we used a rabbit IgG antibody (ab313801 from Abcam) Following the immunoprecipitation, the samples were treated with proteinase K. The IP-RNA was first fragmented and used to create a library using an RNA adapter on the 3' end of the target RNA.