GSM8827566: LTB10; Homo sapiens; RNA-Seq
Tryzol and Thermoscientific GeneJET RNA Cleanup and concentration micro kit. Library was performed according to the manufacter's instructions (Truseq Stranded mRNA protocol, Illumina). The sequencing library is prepared by random fragmentation of the cDNA sample, followed by 5' and 3' adapter ligation. Alternatively, "tagmentation" combines the fragmentation and ligation reactions into a single step which greatly increases the efficiency of the library preapration process. Adapter-ligated fragments are then PCR amplified with a PCR primer solution which anneals to the ends of each adapters. The library templated undergo quality control and quantification process.