16S rRNA sequencing of WildR microbiome: WildR-Ba-exo-titration, WildR + 100x Ba, day 27 feces, mouse 2
gDNA was extracted from thawed fecal pellets via mechanical disruption, purified by phenol extraction and qiagen column clean up. V3-V4 16S rRNA sequencing libraries were prepared following the Illumina Protocol for 16S metagenomic sequencing (Part # 15044223 Rev. B) using the suggested primers. 16S PCR was performed in triplicate.