Identification of beta-catenin-associated RNAs in myeloid leukaemia cells through RIP-seq
Identification of beta-catenin-associated RNAs in myeloid leukaemia cells through RIP-seq
Myeloid cell lines (K562 and HEL) were treated with DMSO or CHIR99021 (CHIR) overnight. Subsequently, beta-catenin RIP was performed in treated cells. CHIR99021 was used to provide beta-catenin stabilisation, which leads to Wnt/beta-catenin signalling activation. DMSO was used as a vehicle control.  Magna RIPâ„¢ RNA-Binding Protein Immunoprecipitation Kit (17-700; Sigma-Aldrich) TruSeq Stranded Total RNA libraries made from RNA purified using a RIP protocol