Biomolecular Condensate Coronas from Nanoparticle Intracellular Processing Are Transferred Between Cells Allowing Cytosolic and Nuclear Access
Biomolecular Condensate Coronas from Nanoparticle Intracellular Processing Are Transferred Between Cells Allowing Cytosolic and Nuclear Access
Cells were trypsinzed and collected in a fresh tube. The cells were pelleted by 1000 g for 5 min at 37C. The cell pellets were washed in PBS and re-pelleted. A549 cells were washed with PBS and trypsinzed and centrifuged to collect the cell pellets. A549 cells were cultured in normal growth media. A549 cells were untreated. We used QIAGEN miRNeasy Micro Kit (Cat. No. 217084) to extract RNA. MGIEasy RNA Library Prep Set