GSM8759307: siSF1-1, NPC line 2; Homo sapiens; RNA-Seq
Total RNA was isolated using the NucleoSpin® RNA isolation kit (Macherey-Nagel). RNA integrity numbers (RINs) were consistently above 9.5. Library preparation including end repair, tailing, ligation, and purification, was performed using 500 ng of RNA in a 50 µL reaction volume.