GSM8457941: [ATAC-Seq] chd4_shluci_rep2; Mus musculus; ATAC-seq
Cells were resuspended in 50 μL lysis buffer. Then the suspension of nuclei was centrifuged 500 g at 4°C for 10 min. The pellet was resuspended by adding 50 μL transposition reaction mix, and incubated at 37°C for 30 min. Finally, DNA was isolated using MinElute PCR Purification Kit. ATAC-seq libraries were constructed and  purified with AMPure XP beads.