RNA-Seq of Mus musculus: male kidney, WT, cisplatin-induced AKI
Total RNA was extracted from mouse kidney tissue using TRIzol and assessed for quality and quantity. mRNA was purified, fragmented, and converted into cDNA, followed by adaptor ligation, PCR amplification, and library purification. Sequencing libraries were quality-checked and sequenced on the Illumina NovaSeq 6000 platform (PE150).