GSM8604937: hepatocyte_Mttp_KO_CM_4; Mus musculus; RNA-Seq
Total RNA was isolated from different organs using a TRIZOL reagent. The RNA was purified using  RNA purification kit. RNA concentrations were measured using NanoDrop (Thermo Scientific™, USA, #ND-2000). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dTTP. It was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.