Metagenomic Decoding of Microbiomes in Food Wastewater
Sequencing libraries were prepared from food wastewater microbial DNA using the NEBNext Ultra DNA Library Prep Kit for Illumina, following the manufacturers instructions, and unique index codes were added. Library quality was evaluated using the Qubit 2.0 Fluorometer (Thermo Scientific) and Agilent Bioanalyzer 2100 system. High-throughput sequencing was then performed on an Illumina platform, generating 250 bp paired-end reads to capture the complete microbial community for metagenomic analysis.