Metagenome of diatoms and bacteria from Antarctic sea ice bottoms
After assessing the quality of the DNA samples using the Qubit dsDNA Assay Kit, samples that passed quality control were used to construct DNA libraries following the manufacturers protocol of the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, USA). The constructed libraries were then sequenced on the Illumina HiSeq X Ten platform using 150 bp paired-end sequencing.