GSM8459678: APOE4 primed + PolyI:C treated_3; Homo sapiens; RNA-Seq
Cells were lysed using Ambion Trizol Reagent (Invitrogen) under RNase-free conditions for RNA extraction. The lysate was separated into an aqueous RNA phase using chloroform and centrifugation. The RNA pellet was washed with RNase-free 75% ethanol, air-dried, and resuspended in RNase-free water. RNA libraries for RNA-seq were prepared using TruSeq Stranded mRNA Library Prep Kit following manufacturer's protocols