Plasmid library RTW554, p11-3_8xN_PAM-site1, treated with SpyCas9, replicate 2
Library design is described in Walton, R.T., Hsu, J.Y., Joung, J.K. et al. Scalable characterization of the PAM requirements of CRISPRCas enzymes using HT-PAMDA. Nat Protoc 16, 15111547 (2021). https://doi.org/10.1038/s41596-020-00465-2