GSM8654672: LAA_pt2_Neu_3; Homo sapiens; OTHER
Retrieved thrombi were fixed in 4% paraformaldehyde, embedded in paraffin, and sectioned at 4 μm. Tissue slides were hybridized with Human Whole Transcriptome Atlas probes, followed by staining with morphology markers. For each tissue sample, 3–6 regions of interest (ROIs) were selected and segmented into CD68+, CD163+, or NE+MPO+ groups based on immunofluorescence signals. Oligonucleotides from the segmented areas were released by UV light and indexed using Illumina i5/i7 dual indexing primers. PCR products were pooled and purified using AMPure XP beads (Beckman Coulter). The pooled libraries were sequenced at 100-bp paired-end read length on an Illumina NovaSeq 6000 platform.