Nanopore sequencing of pea aphid Rickettsia endosymbiont
High molecular weight DNA was extracted from pea aphid insects harboring Buchnera aphidicola and Rickettsia endosymbionts using the QIAGEN Genomic DNA extraction kit as recommended with the bacterial genomic tip 20/G procedure. DNA was sequenced on the Nanopore PromethION platform generating 2,769,975 reads with an N50 of 20,962 base pairs.