ONT sequencing of Drosophila melanogaster: iso-1 adult males, control
Genomic DNA was extracted from 60 adult Drosophila melanogaster iso-1 males by phenol-chloroform extraction. ONT libraries were prepared using the SQK-LSK114 protocol on R10.4.1 flow cells. The library was divided into two equal portions. The control portion was loaded using standard protocols. For the SSB portion, recombinant E. coli SSB (Promega M3011, 3.16 g/L) was serially diluted in 10 mM Tris-HCl and added to the loading mix immediately before flow-cell loading. Basecalling was performed with Dorado v0.7.1 using a minimum quality score threshold of 10.