GSM9023057: CP9 strain, planktonic, 90 min, rep 1; Candida parapsilosis; RNA-Seq
The conical tubes were centrifuged at 4,000 x g for 5 minutes and the supernatant was aspirated. Pellets were snap-frozen and stored at -80°C until RNA extraction. Total RNA was extracted using the Ribopure RNA Purification Kit for Yeast (AM1926). mRNA was separated from total RNA using the NEBNext Poly(A) mRNA Isolation Module (E7490L). 500 ng of purified mRNA were made into cDNA libraries using the NEBNext Ultra II RNA Library Prep Kit for Illumina (E7775L).