GSM8827184: AH1; Mus musculus; RNA-Seq
Total RNA was extracted from the tissue using TRIzol Reagent according to the manufacturer's instructions (Invitrogen) and genomic DNA was removed using DNase I (Takara). RNA quality was determined by 2100 Bioanalyser (Agilent Technologies) and quantified using the ND-2000 (NanoDrop Technologies). The transcriptome library was prepared following TruSeqTM RNA sample preparation Kit from Illumina (San Diego, CA) using 1μg of total RNA. Libraries were size selected for cDNA target fragments of 300 bp on 2% Low Range Ultra Agarose followed by PCR amplified using Phusion DNA polymerase (NEB) for 15 PCR cycles. After quantified by TBS380, the paired-end RNA-seq sequencing library was sequenced with the Illumina NovaSeq 6000 sequencer (2 × 150bp read length). The raw paired-end reads were trimmed and quality was controlled by fastp with default parameters.