Z-16-F (CRX2068864; CRA031444)
To ensure sample integrity and data quality, standardized procedures were followed throughout the collection process, with unique identifiers assigned before and after sampling. Fecal samples were transferred into sterile 1 ml cryovials and immediately stored at -80 Celsius. Once all samples were collected, they were shipped to Novogene Co., Ltd. (Beijing, China) for DNA extraction, library preparation, and metagenomic sequencing. Paired-end shotgun metagenomic sequencing (2x150 bp) was performed on the Illumina NovaSeq 6000 platform.