GSM8994823: pm1_455; Mus musculus; RNA-Seq
Bone marrow cells from 5TGM mice were removed erythrocytes by incubating with RBC lysis buffer. Subsequently 1×PBS (0.04% BSA) was used to resuspend the cell precipitate and then centrifuged at 4°C 300g for 5min (repeated twice). The cell activity was detected by trypan blue staining, and it was required to be >85%. The number of cells was counted using a hemocytometer, and the cell concentration was adjusted to 103 cells/μL. Single-cell suspension was added to the 10x Chromium chip according to the instructions for the 10×Genomics Chromium Single-Cell 3' kit (V3), with the expectation of capturing 10,000-15,000 cells. cDNA amplification and library construction were performed according to standard protocols.