GSM8654908: H2AZ_WT_DMSO_Rep22; Homo sapiens; ChIP-Seq
Cells  were fixed with 1% formaldehyde for 14 min,  after quenching and cell lysis. Chromatin was sonicated using a Branson Sonifier 450 with a microtip (40% amplitude, 3 s on, 10 s off, 5 min of total pulse time). ChIP-seq libraries were constructed using the Ovation Ultralow System V2 1–16 Kit (NuGEN Technologies) according to the manufacturer's protocol. Briefly, 2ng of ChIP DNA was end-repaired and ligated to specific adaptors. The adapted DNA was purified using Agencourt® Ampure XP Beads (Beckman Coulter, Cat. #A63881), followed by PCR amplification (13 cell cycle for 2ng DNA input). The amplified DNA was purified again with Ampure XP Beads. The quality of the library, including the library size distribution, purity, concentration was assessed at the Sequencing Facility Center of University of Wisconsin-Madison. Qualified libraries were sequenced on an Illumina Novaseq X Plus platform with paired-end reads of 150 bases.