GSM8458157: 0h_Dppa3_rep2; Mus musculus; OTHER
Cells was cross-linked with 2% formaldehyde and quenched with 0.2 M glycine. DNA was then digested with MNase, and then subjected to end repair, promixity ligation and reverse cross-linking. 500 ng of DNA was used as a template and linearly amplified using 5' biotin-labeled probes. The ssDNA was enriched using Dynabeads™ M-280 magnetic beads. MNase-4C libraries were constructed with specific primer pairs, whereas forward primers contain Illumina P5 with an index and sequences near a specific viewpoint and reverse primers contain Illumina P7 with an index and sequences matching the adapter.