16Sseq of pig gut metagenome: positive control
The colonic samples were used for extraction of total bacterial DNA using a DNA kit. amplification of the 16s rRNA gene was performed from V3 to V4 regions by PCR using bacterial universal primers of 338F (50-ACTCCTACGGGAGGCAGCAG-30) and 806R(50-GGACTACHVGGGTWTCTAAT-3), with a unique eight-base sequence barcode for each sample. Purified amplicons were pooled in equimolar and paired end sequenced on an Illumina MiSeq platform.