GSM8860449: Bulk_RNA_KPTN_tumor1; Homo sapiens; RNA-Seq
Cell pellets of cultured organoids were collected and stored in Trizol for total RNA extraction using Zymo Direct-zol RNA Miniprep Kit (Zymo Research, Irvine, CA, R2063) following manufacturer's instructions. Tumor tissues were flash frozen in liquid nitrogen and ground into fine powder with the addition of Trizol, followed by RNA extraction using Zymo Direct-zol RNA Miniprep Kit. Indexed cDNA libraries were prepared using the Illumina® Stranded mRNA Prep Ligation kit (Illumina, San Diego, CA, 20040532) following manufacturer's instructions.