Gene expression profile from single cell level of retinal cells from optic cup of injected Abca4KO mice with miR-181a/b sponge therapeutic
Gene expression profile from single cell level of retinal cells from optic cup of injected Abca4KO mice with miR-181a/b sponge therapeutic
Abca4KO mice were injected with miR-181a/b sponge in one eye and with a control vector in the controlateral. Subretinal injection were performed at 2 months of age and samples collected and analyzed at 4 months post-treatment. The cell suspension was loaded onto a Chromium Single Cell G Chip Kit (10x Genomics) and processed with the Chromium Single Cell Controller (10x Genomics) to produce single-cell gel bead emulsions, following the manufacturer's guidelines.  cDNA and final libraries were constructed using the Single Cell 3â€² Library and Gel Bead Kit V3.1 (10x Genomics). The quality of the cDNA was assessed with High Sensitivity D5000 ScreenTape using the Agilent 4200 TapeStation system (Agilent Technologies), while the final libraries were evaluated with High Sensitivity DNA D1000 ScreenTape (Agilent Technologies).