BHTC-1 (CRX2064211; CRA031329)
DNA from soil samples was extracted using a DNeasy PowerSoil Kit, and the bacterial 16S rRNA gene (V4 region) was amplified with barcoded primers. Then the libraries were constructed through end-repair, A-tailing, adapter ligation, and purification using AMPure XP beads for MiSeq sequencing.