GSM8603631: Thymocytes, Sham-biol rep 1; Mus musculus; RNA-Seq
For thymocytes extraction, thymus tissues were homogenized. The homogenized tissue was passed through a 40 µm cell strainer to obtain a single-cell suspension, which was then filtered to separate thymocytes from remaining stromal cells. The resulting thymocyte suspension was used for further downstream analyses. The mRNA was enriched using magnetic beads with Oligo (dT) and subsequently fragmented using Fragmentation Buffer. First and second strands of cDNA were synthesized from the fragmented mRNA, and the cDNA was then purified. The double-stranded cDNA underwent end repair, A-tailing, and adapter ligation, followed by size selection with AMPure XP beads. Finally, PCR amplification was performed to generate the cDNA library.