ChIP-Seq of Fusarium graminearum
The strain FgSR::FgSR-GFP was cultured in YEPD at 25C for 36 h in a shaker with 180 rpm, and then treated with 0.25 mg/ml MBOA for another 4 h. Fresh mycelia were cross-linked with 1% formaldehyde for 10 min, and then the reaction was stopped with 125 mM glycine for 5 min. Subsequently, samples were grounded in liquid nitrogen and suspended in lysis buffer