RNA-Seq of homo sapiens: adult female thymus CD4
TCR libraries were prepared from RNA using the SMARTer Human TCR / Profiling Kit v1 (Takara Bio) according to the manufacturers instructions. Amplicons were purified with AMPure XP beads (Beckman Coulter), quantified, and their integrity was assessed using an Agilent 2100 Bioanalyzer (DNA 1000 kit) or a Tapestation 4200 (Agilent, D1000 ScreenTape).