Infected with HIV-1 P90A AC-1 capsid mutant in the presence of expressed CypA with NLS
infected DNA was digested with AvrII, BamHI, NheI, and SpeI restriction enzymes. HIV-1 integration libraries were prepared by ligation-mediated PCR with long primers that anneal HIV-1 U3 EIAV LTR and linker. PCR products were subjected to Illumina sequencing to determine HIV-1 sites.