Illumina genome sequencing of Rhexocercosporidium sp. Me07
The sequencing experimental procedure was carried out in accordance with the standard protocol provided by Illumina, which includes processes such as sample quality testing, library construction, library quality testing, and library sequencing. After the genomic DNA of the sample passed the quality test, the DNA was fragmented using a mechanical shearing method (ultrasonication). The fragmented DNA was then subjected to fragment purification, end repair, addition of an 'A' base to the 3' end, and ligation of sequencing adapters. The DNA fragments were size-selected using agarose gel electrophoresis, followed by PCR amplification to form the sequencing library. The constructed library was first subjected to quality control testing, and libraries that passed the quality control were sequenced using the Illumina Novaseq6000 platform.