GSM8720697: HeLa cells, G3BP1-GFP, AS+FA, rep1; Homo sapiens; RIP-Seq
After GFP enrichment, 500 μL TRIzol (Thermo, 15596026) was added to the beads to extract RNA, followed by FA crosslink reversal at 54°C for 2 hours and 70°C for 30 minutes. RNA was subjected to end-repair, reverse transcription, adapter ligation, PCR amplification, and size selection by gel electrophoresis. Libraries were sequenced on the Illumina platform with a 6G throughput per sample. Fastp was used to trim adapters and filter low-quality reads (Q < 20) to generate the final SG transcriptome dataset.