GSM8828586: mtDT, Family F, IPW-5371 + RT + anti-PD-L1  #1, d7; Mus musculus; RNA-Seq
Qiagen RNA Easy Kit and following standard Protocol and DNAase I cleanup protocol listed in the Appendix.  Approximately 15 ul and 1000ng of RNA was used Samples were quantified using  Qubit 4 flurometer and quality was assessed using a 4200 TapeStation.  Any  samples with >10% DNA contamination were  treated with TURBO Dnase to remove DNA contamination. QIAGEN FastSelect rRNA HMR Kit was then used for rRNA depletion following manufacturer's protocol. RNA sequencing libraries were constructed with the NEBNext Ultra II RNA Library Preparation Kit for Illumina by following the manufacturer's recommendations