GSM9211086: H3K27ME3 WT ESC2 (Chip); Mus musculus; ChIP-Seq
The cells were crosslinked with 1% formaldehyde for 10' at room temperature and next quenched by addition of glycine (125 mM). The samples were sonicated with Bioruptor Plus (Diagenode). libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) and NEBNext Multiplex Oligos for Illumina (NEB)