Illumina MiSeq sequencing of SAMD01821322
Tumor tissues were homogenized in Sepasol-RNA I Super G (Nakarai, 09379-84) at 2800 rpm, followed by sonication for tissue disruption. Total RNA was extracted according to the manufacturer's protocol. The RNA samples were treated with HT1 Hyb Buffer 1 and 1 M NaOH and denatured at 25°C for 5 minutes. RNA sequencing libraries were prepared according to the standard Illumina protocol and sequenced on the Illumina MiSeq platform using the MiSeq v2 Reagent Kit to generate 300 bp paired-end reads.