GSM9352982: DMD Cardiomyocytes, Prednisolone, Replicate 2; Homo sapiens; RNA-Seq
Cells were harvested and resuspended directly into RLT + 1%BME and snap frozen using the indicated markers. Total RNA was purified using the Quick-RNA MicroPrep Kit (Zymo Research) Purified RNA was used as input for the SMART-seq v4 cDNA synthesis kit (Takara) and used as an input for the NexteraXT kit with the NexteraXT indexing primers (Illumina).