RNAseq data of mixed reproductive tissues (inflorescence meristems, flower buds at various developmental stages, open flowers, fruits) of Hibiscus trionum, New Zealand naturalised race, forward reads
Whole bud, flowers, inflorescence meristems and fruit tissue were collected and flash frozen in liquid nitrogen. Per species/population, three biological replicates were collected with each biological replicate corresponding to one individual plant. Frozen samples were homogenized using a pestle and mortar and total RNA was extracted using the Spectrum Plant Total RNA Kit (Sigma STRN250). ~40 mg of tissue was used for each RNA extraction. An on-column DNase I treatment (Sigma DNASE70) was performed between the first two column washes. RNA was eluted in 30 l nuclease-free H2O (Promega MC1191) and purity was analyzed using a NanoDrop (ThermoFisher Scientific) and RNA ScreenTape (Agilent 5067-5576, 5067-5577, 5067-5578) on the Agilent TapeStation 2200 system.  Samples with minimum 1.7 g total RNA (>50 ng l-1), and A260/280 value of 2.0 were shipped on dry ice to Azenta Life Sciences, Leipzig (formerly GeneWiz) for RNA-seq library preparation and sequencing. Library preparation was performed with polyA selection and sequencing using an Illumina HiSeq machine (2x 150 bp configuration, ~350 M raw paired-end reads per lane, at least 30M reads per sample).