Bulk RNA-seq of mus musculus B16 melanoma
tumor tissues were excised into ~30 mg pieces using razor blades and submerged into RNA protect tissue reagent (Qiagen) overnight at 4 C. Tissues were then transferred to -80 C and pulverized using a liquid nitrogen cold hammer. Total RNAs were then isolated using the Qiagen RNeasy Mini kit according to the manufacturers protocol. mRNA enriched sequencing was performed by Genome Quebec