GSM9679222: A1; Mus musculus; RNA-Seq
Cells were lysed in TRIzol (Invitrogen, 15596018CN) and RNA was extracted according to the standard protocol. Briefly, after different treatments, cells were collected into 1.5 mL tubes (Axygen, MCT-150-C). RNAs were washed with 75% ethanol. For each sample, the extracted RNA was dissolved in 20 μL DEPC water. RNA libraries for RNA-seq were prepared using Stranded mRNA Library Prep Kit following manufacturer's protocals.