Single cell RNA-seq of mus musculus: CD4 cells isolated from adult female lungs infected with C. neoformans UgCl223 at 14 DPI
Briefly, lungs were perfused with cold PBS and digested into a single cell homogenate with Collagenase A. Lymphocytes were then isolated via a 67%/40% Percoll density gradient, and followed with CD4 T-cell isolation via negative selection using the EasySep Mouse CD4+ T cell Isolation Kit (StemCell). Single cell RNA sequencing libraries were prepared via a Chromium Single Cell Controller (10x Genomics) using the Chromium Single Cell 5 Library and Gel Bead v2 dual index kits (10x Genomics) according to manufacturer's instructions.