L1td1 Eclip-seq in ESC Rep (CRX2026181; CRA030513)
The lysates were incubated with 10 ug anti-L1td1 (Proteintech, 21528) conjugated to Dynabeads with protein A and G for 4 hours, followed by washing, end repair, and 3' adaptor ligation. The protein-RNA complexes were eluted from the beads, resolved by denaturing gel electrophoresis, and transferred onto a nitrocellulose membrane. Library preparation was performed by DIATRE Biotechnology (Shanghai, China) as in the original protocol. submitted_instrument_model: Illumina HiSeq X Ten