GSM8574919: SI, Cystine rep1, scRNAseq; Mus musculus; RNA-Seq
Based on FACS analysis, single CD45+ cells from intra-crypts of mice fed with control and high-cysteine diets were sorted into 1.5 ml low-binding tubes with 500 ul FACS buffer. Single cells were processed through the GemCode Single Cell Platform using the GemCode Gel Bead, Chip and Library Kits (10x Genomics, Pleasanton) as per the manufacturer's protocol. The loading cell number is 18,000 for each sample The cells were then partitioned into Gel Beads in Emulsion in the GemCode instrument, where cell lysis and barcoded reverse transcription of RNA occurred, followed by amplification. Amplified cDNA was then used for 5' gene expression library construction Amplified cDNA was fragmented and end-repaired, double-sided size-selected, PCR-amplified with sample indexing primers, and double-sided size-selected