GSM8458027: H3K27ac_MNase_HiChIP_IAA_rep2; Mus musculus; OTHER
Nuclei of crosslinked cells were separated. DNA was then digested with MNase, and then subjected to end repair and promixity ligation in situ. The nuclei were then resuspended in 100 µL nuclear Lysis Buffer. ChIP was performed with indicated antibody. ChIPed DNA  was reverse crosslinked, purified with MinElute PCR Purification Kit and further subjected to biotin IP. The DNA sample derived form biotin immunoprecipitates were subjected to PCR amplification followed by size selection between 200 bp to 800 bp.