GSM9261490: PROTAC_Day5_Rep3; Homo sapiens; RNA-Seq
Total RNA was extracted from PRC2-perturbed cells treated with DMSO, PROTAC, or UNC1999 at day 5 of the transition, with three biological replicates per condition. Cells were washed with PBS and harvested in RLT buffer from the RNeasy Mini Kit (Qiagen Cat No: 74104) and stored at -80C. Once thawed, the suspension was passed through a QIAshredder (Qiagen Cat no: 79656) and the RNA purified using the RNeasy Mini Kit following manufacturer's instructions. Contaminating DNA was removed using the Turbo DNA free kit (ThermoFisher Scientific Cat No: AM1907) following manufacturer's instructions. RNA-seq libraries were prepared using the Watchmaker RNA Library Prep Kit with ribosomal RNA depletion following manufacturer's instructions.