GSM9530492: tMCAO 6h _3; Mus musculus; Hi-C
Peri-infarct cortical tissue and corresponding sham tissue were dissected, flash-frozen in liquid nitrogen. 36 mice were used to generate n = 4 pooled biological replicates/condition (sham, 6h reperfusion and 24h reperfusion), with each replicate comprising 3 randomly assigned animals. Hi-C libraries were generated using the Arima-HiC kit (A510008) and the Arima Library Prep Module (A303011) following manufacturer's protocols. Briefly, tissue was crosslinked, pulverized using liquid nitrogen, digested with the Arima enzyme cocktail, proximity-ligated, reverse-crosslinked and purified. DNA (∼3 µg) was fragmented to 200–600 bp, and 200 ng of biotin-enriched DNA was size-selected, indexed and amplified. Libraries were sequenced on an Illumina NovaSeqPlus platform (150 bp paired-end), with a target of ≥ 600 million reads per library.