RNA-seq from Unio delphinus
Total RNA was extracted from the gill tissues of all individuals using the NZYTech total RNA isolation kit and an on-column DNase I treatment. RNA concentration (ng/l) and quality measurements (OD260/280 ratio values) were obtained using a DeNovix DS-11 Series Spectrophotometer/Fluorometer, and RNA integrity was evaluated with a 1% agarose gel. The extracted total RNA from the 48 samples was sent to Macrogen, Inc. to build strand-specific libraries. The insert size was 250300bp, and sequenced using 150bp paired-end reads on the Illumina HiSeq 4000 platform.