GSM9312241: Caulobacter crescentus, wild-type; Caulobacter vibrioides; ChIP-Seq
Cells were crosslinked with 1% formaldehyde and sonication was carried out using a Covaris ultrasonicator with the following settings: temperature 6°C, duration 3600 seconds, peak power 75.0, duty % factor 10.0, cycles per burst 200, average power 7.5, delay 60 seconds. All of the cleared sonicated lysate was used for ChIP without taking an input sample out first. DNA was prepared for Illumina sequencing using the NEBNext UltraExpress DNA library preparation kit (New England Biolabs) according to the manufacturer's instructions and 50 bp paired-end sequencing was carried out on a NovaSeq 6000 (Illumina).