Whole-genome sequencing of Brachypodium distachyon: control line at M5 generation
Chemical mutagenesis of seeds: 0 mM of sodium azide followed by 4 generations of self-fertilization in the greenhouse. Whole genome sequencing: collection of juvenile leaf tissue (about 150 mg of leaf tissue, 5 weeks after sowing) followed by DNA extraction and DNBSEQ (paired-end sequencing, 150-bp reads)