GSM9235591: Multiplexed sublibrary 1; Mus musculus; RNA-Seq
Mouse hind limbs were briefly cleaned to remove skin and muscle surrounding the joint. Knee joints  were then  harvested by trimming approximately 1mm of tissue from the ends of both long bones and enzymatically digested with pronase followed by liberase enzymes (Cat# 5401127001)  at 37ºC for 1 hr and 6 hrs, respectively, as previously described. Single-cell suspensions were immediately fixed using the Evercode Cell Fixation v1 kit (Parse Biosciences, Seattle) according to manufacturer's instructions. Fixed samples were stored at -80°C until processing. Library preparation was performed using the Evercode WT Mega v1 kit (Parse Biosciences, Seattle) following manufacturer's instructions. Sequences from all samples were barcoded and combined into one sublibrary which was sequenced.