GSM8966477: 21dpf; Danio rerio; RNA-Seq
For all datasets, the cell suspensions were depleted for dead cells using LeviCell 1.0 device (Levitas Bio), counted again with MOX Z cell counter and diluted according to manufacturer's protocol to obtain 10.000 single-cell data points per sample.  Each sample was run separately on a lane in Chromium controller with Chromium Next GEM Single-cell 3ʹ Reagent Kits v3.1 (10xGenomics).  Single-cell RNAseq library preparation was done using standard protocol. Library preparation was conducted according to the manufacturer indications.