RNAseq of the ecDNA-positive colon cancer cell line COLO 320DM with and without hydroxyurea treatment
RNAseq of the ecDNA-positive colon cancer cell line COLO 320DM with and without hydroxyurea treatment
Cells were washed with 1x PBS, trypsinized (Gibco) and centrifuged at 300xg for 5 min and subjected for RNA extraction RNA was extracted using the RNeasy Mini Kit (Qiagen, 74104). Genomic DNA was removed by DNase treatment using the RNase-Free DNase Set (Qiagen,79254). RNA-seq libraries were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) at the Berlin Institute of Health (BIH) Genomics Technology Platform.