Revealing the microbial diversity present in septic tank wastewater using a metagenomic approach
Septic tank wastewater (STWW) sample was collected and subjected to metagenomic DNA extraction to explore the microbial community composition. Sequencing libraries were prepared using the NEBNext Ultra DNA Library Prep Kit for Illumina following the manufacturers instructions. Library quality was assessed using Qubit 2.0 Fluorometer and Agilent 2100 Bioanalyzer. The qualified library was sequenced on the Illumina MiSeq platform to generate 250 bp paired-end reads for downstream taxonomic and functional analysis.