Plasmodium falciparum Amplicon deep-sequencing using Pf-SMARRT panel
All P. falciparum positive samples were genotyped in duplicate using Pf-SMARRT, a 24-locus deep sequencing amplicon panel that targets key markers on drug resistance genes, including multidrug-resistance 1 (Pfmdr1), chloroquine resistance transporter (Pfcrt), dihydrofolate reductase (Pfdhfr), dihydropteroate synthase (Pfdhps), and 21 validated and candidate mutations in Pfk13.