GSM8758956: Jurkat_Orpiment_rep2; Homo sapiens; RNA-Seq
RNA was isolated from 5x10E6 cells using the SV Total RNA Isolation System (Promega #Z3100). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was prepared through end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina platforms, according to effective library concentration and data amount.