GSM9312609: C9-ALS iMNs, IgG.; Homo sapiens; OTHER
The genomic DNA was purified and digensted with a mix of restriction enzymes [HindIII (ThermoFisher, FD0504), EcoRI (ThermoFisher, FD0274), XbaI (ThermoFisher, FD0684), and BamHI (ThermoFisher, FD0054)] at 37 °C for 2 h. The digested samples were split into two partsparts, and one part was treated with RNase H (NEB, M0297S) for 37 °C for 2 h, followed by purification with Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v) (ThermoFisher, 15593031). The libraries were constrcuted  using the NEBNext® Ultra™ II DNA Library Prep Kit complemented with  NEBNext Multiplex Oligos for Illumina by following the manufacturer's manual.