RNA from Developmental analysis of  DNA Methylation in the Parasitoid Wasp Nasonia vitripennis
RNA from Developmental analysis of  DNA Methylation in the Parasitoid Wasp Nasonia vitripennis
For RNA samples 400 embryos, 30 larvae, 10 prepupa, 10 pupa and 10 adults were used per replicate (3 replicates for each stage). Samples were extracted using the Tri-Reagent (Sigma Aldrich) protocol, with adjustments for TURBO DNase TM treatment and removal outlined in the paper. RNA concentration was measured using the QubitTM RNA BR Assay Kit (ThermoFisher) and the integrity of RNA was assessed on a Agilent 2100 Bioanalyzer using the Agilent RNA 6000 Nano Kit (Agilent). RNA samples collected in the studies were converted to cDNA by BGI genomics (Hong Kong). Library preparation of these cDNA fragments was also prepared by BGI Genomics (Hong Kong).