GSM8509006: S133A-CREB cells, control, RNA-seq; Mus musculus; RNA-Seq
Total RNA was isolated using TRIzol reagent (Invitrogen),RNA purity and quantification were evaluated using the Qubit4.0(Thermo Scientific, USA); RNA integrity was assessed using Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA); The RNA-seq libraries were constructed using Hieff NGS® Ultima Dual-mode mRNA Library Prep Kit for Illumina® ( Yeasen Biotechnology ,Shanghai). RNA-seq was performed on DNBSEQ-T7 platform (Geneplus-Shenzhen, Shenzhen, China).