RNA-Seq of Pterinochilus murinus: adult female venom glands
Starting material (200 ng total RNA)was used to prepare the library according to the published protocol(dx.doi.org/10.17504/protocols.io.yxmvmkpxng3p/v1). The sequencing library for OxfordNanopore PromethION was created using the direct cDNA sequencing kit SQK-DCS109(amplification-free), according to the manufacturers recommendations, and run in a FLO-PRO002 (R9.4.1) flow cell. Raw data were basecalled using Guppy v6.5.7. (guppy basecaller) inhigh-accuracy (HAC) mode with a qscore cutoff of 7.