scRNA-Seq of mus musculus: adult male lung
Male C57BL/6J mice aged 6-8 weeks (purchased from VITAL RIVER Laboratory Animal Technology Co., Ltd.) were maintained in specific pathogenfree (SPF) facilities under a 12h light/dark cycle with ad libitum access to food and water. All animal procedures were approved by the Institutional Animal Care and Use Committee and were conducted in accordance with the Guide for the Care and Use of Laboratory Animals. The mice were randomly allocated to the shamexposed (sham) and cigarette smokeexposed (smoke) groups. A wholebody exposure system was used to deliver mainstream smoke from commercial cigarettes (3 cigarettes per session, 2 sessions per day, 5 min per cigarette) for 4 consecutive weeks, thereby establishing a model of chronic smokeinduced lung injury. The mice were euthanized under anesthesia 27 h after the final exposure, and lung tissues and bronchoalveolar lavage fluid (BALF) were collected for subsequent analyses.