GSM8720453: MDAMB468 cells, shNT, biol rep 2; Homo sapiens; RNA-Seq
Total RNA was isolated from cells using miRNeasy Mini Kit (Qiagen) including on-column DNase digestion following the manufacturer's protocol. 1µg of total RNA was used to construct the libraries for sequencing. RNA libraries for RNA-seq was prepared using KAPA Riboerase kit (Roche) according to manufacturer's instructions.