PacBio reads
High molecular weight DNA was extracted using the MagAttract HMW DNA kit (Qiagen), following the protocol of Sanger Tree of Life HMW DNA Extraction: Manual Plant MagAttract v.4 protocol (Jackson & Howard 2023). For long-read sequencing, a ~20 Kb library was prepared using SMRTbell prep kit 3.0 (PacBio) and sequenced on a Sequel IIe (PacBio) using SMRT Cell 8M Tray (PacBio).