GSM9756116: donor3 + PBS; Homo sapiens; RNA-Seq
Total RNA from granulocytes and NK cells was extracted with TRIzol reagent (Invitrogen). The purity of the RNA was evaluated using agarose gel electrophoresis and a NanoDrop 2000 spectrophotometer (Thermo Scientific), and RNA integrity was assessed with an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The RNA-seq library was constructed from 100 ng of RNA using the VAHTS Universal V10 RNA-seq Library Prep Kit (Premixed Version) according to the manufacturer's instructions, and then sequenced on the DNBSEQ-T7 platform.