GSM9235974: Colon, tumor24; Homo sapiens; RNA-Seq
Total RNA was extracted from colonic tissue samples using TRIzol reagent kit (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's protocol. RNA quality was assessed with an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA) and by RNase-free agarose gel electrophoresis. We performed RNA sequencing on the samples of included patients using SureSelectXT RNA Direct Library Preparation Kit.