Pacbio data of Chlorella sp. MEM25
Single molecule real time sequencing (SMRT) libraries and Illumina short read libraries were constructed using fresh mid-log phase algae (BioMarker, China), followed by sequencing with the PacBio Sequel II and HiSeq 2000 platforms. The Hi-C library and sequencing were conducted with the Illumina HiSeq platform (Novogene, China). Multiple approaches have been employed to guarantee the quality of genome sequences. PacBio and Hi-C data, Hifiasm (version 0.16.1-r375) was used for subgenome splitting. SMRT sequencing data were assembled using Falcon (version 0.3.0) and CANU (version 2.1.1). 3D-DNA (version 180922) and ALLHiC (version 0.9.8) were used for draft genome correction and genome assembly, respectively. Finally, Juicebox (version 1.11.08) and Pilon (version 1.24) were used to correct genome fragments (based on the chromosome contact frequency matrix) and base errors of the genome with Illumina and RNA-seq data, respectively. The Hi-C interaction frequency matrix was analyzed by Homer, FitHic (version 2.0.8), HiCExplorer (version 3.7.2), and HiCCUPS (version 5.14).