GSM8455474: 1dpi; Mus musculus; RNA-Seq
Small intestines were flushed with ice-cold PBS, cut longitudinally into roughly 2-mm-long pieces, rinsed with ice-cold PBS, and incubated in 2mM EDTA at 4°C for 40min with continuous shaking. The whole-epithelial segments were isolated by vigorous shaking in ice-cold PBS, and enriched for crypts by passing dissociated epithelial segments through a 70µm mesh cell strainer. 10x Genomics Single Cell Gene Expression