GSM8603342: adult_vm_2; Mus musculus; miRNA-Seq
To purify subsets of CD44hiCD122hi and CD44loCD122lo CD8+ T cells from neonatal and adult gBT-I mice, CD8+ T cells were enriched using anti-CD8α microbeads (Miltenyi Biotec) and were subsequently labeled with antibodies against CD4, CD8, CD44, and CD122 and sorted to >95% purity on a fluorescence-activated cell sorting (FACS) Aria III (BD Biosciences). Libraries were prepared with Illumina TruSeq Small RNA Prep Kit using 50-100ng total RNA as input, size selected for insert sizes ~18-35bp. small RNA-seq