PF3D7_0315600 3' tag with 3xHA-glmS ribozyme genome sequence
Genomic DNA was extracted from 25 mL of mixed-stage P. falciparum parasite culture of clonal PfMD3-3xHA-glmS strain using a commercial kit. Sequencing library was prepared according to manufacturer's instructions for Nanopore sequencing. Whole genome sequencing was performed per manufacturers guidelines using a MinION MK1C, where BAM files were basecalled in duplex with Dorado v0.5.1 using the super accurate model. BAM files were filtered to remove reads less than 200 bp and Q scores <20 prior to alignment to the P. falciparum 3D7 reference genome version 66.0.