GSM8718669: pNE_H_RNA_2; Homo sapiens; RNA-Seq
Cells were lysed with Rneasy lysis buffer (Qiagen) and RNA was subsequently extracted using RNeasy Plus Kit (Qiagen) Libraries were prepared by Novogene. Briefly: messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. Libraries were sequenced on a Novoseq 6000.