TCR-Seq of Homo sapiens: flow-sorted T cells from bronchoalveolar lavage fluid
     We performed TCR sequencing on selected samples of CD4+ and CD8+ T cells      that contained at least 0.5 ng of residual RNA following bulk RNA sequencing.      RNA quality and quantity were measured using a 4200 TapeStation and      a high-sensitivity RNA ScreenTape (Agilent Technologies) before library      preparation using the SMARTer Human TCR α/β Profiling kit (Takara Bio).      In brief, this kit uses a 5′ RACE-like approach to capture complete V(D)J      variable regions of TCR transcripts and primers that incorporate Illumina-specific      adaptor sequences during cDNA amplification. Libraries were then pooled,      denatured and diluted to a DNA solution with a final concentration of 1.8 pM.      PhiX control was spiked in at 20%. Libraries were sequenced on an Illumina      NextSeq 500 instrument using a NextSeq 500/550 Mid Output kit v2.5 (300 cycles)      with a target read depth of approximately 17.39 million aligned reads per sample.     