Full genome sequencing of Micrococcus yunnanensis PolyGone: sodium hydroxide
DNA concentration and purity were measured using a NanoDrop One . Oxford Nanopore Technologies sequencing was performed by Flow Genomics. Library preparation was conducted with the SQK-RBK114.96. Sequencing was performed on a MinION [DC15.1]Isequencer using a FLO-MIN114 MinION/GridION flow cell with default settings, according to manufacturer. Reads were base called using Dodaro [DC16.1]v5.0.0 in super accuracy mode. Reads were assembled de novo using Flye [DC17.1]v2.9.6 into a closed circular chromosome and polished using Medak[DC18.1]a v2.1.0. The assembled genome was annotated using Bakta [DC19.1]v1.11.3