RNA-Seq of Mus musculus : young adult Liver
Total RNA was extracted using TRIzol reagent according to standard protocol, dissolved in water, and treated with DNase I (RNase-free, NEB) for 30 min at 37C. Poly(A)+ RNA was isolated using the Magnetic mRNA Isolation Kit (NEB), then precipitated with ethanol/sodium acetate and resuspended in nuclease-free water. Sequencing libraries were prepared using the MGIEasy Fast RNA Library Prep Set according to the manufacturer's protocol.