GSM9679606: Mice colonic tissues,irradiated,84 hours,bio rep 4; Mus musculus; RNA-Seq
Total RNA was extracted from colon tissues using TRIzol® Reagent (Magen). RNA quality was assessed by A260/A280 ratio (Nanodrop ND-2000) and RIN (Agilent Bioanalyzer 4150). Paired-end libraries were prepared using ABclonal mRNA-seq Lib Prep Kit. Briefly, mRNA was purified from 1 μg total RNA with oligo(dT) magnetic beads, fragmented, and reverse transcribed. Double-stranded cDNA was end-repaired, adapter-ligated, and PCR amplified. Library quality was assessed on an Agilent Bioanalyzer 4150.