GSM8827663: 2.0 MAC_rep3; Mus musculus; RNA-Seq
Total RNA was extracted from whole brain and primary neurospheres using ISOGEN reagent (Fujifilm, Japa). 500 ng of total RNA were ribosomal RNA-depleted using NEBNext rRNA Depletion Kit (New England Biolabs), and converted to Illumina sequencing library using NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs). RNA libraries were prepared for sequencing using standard Illumina protocols.