GSM8605080: Rpb1_gcn5∆_Induced_Replicate 1; Saccharomyces cerevisiae; ChIP-Seq
Sonicated chromatin extracts were immunoprecipitated with anti-histone H3 (Abcam #ab1791) and H3-K18ac (Sigma 07-354), anti-Gcn4 (home-made), anti-TBP (from Joseph Reese's lab), anti-Taf1(rom Joseph Reese's lab), anti-Rpb1 (Biolegend, Cat#664906). Immunoprecipitated chromatin were treated with proteinase K and DNA were extracted with chloroform and precipitated with iso-propanol. Libraries were prepared using DNA Library Prep Kit for llumina (Cat# E7370) from New England Biolabs.