CC-14
The suspension of single cells was transferred onto the 10X Chromium Single-Cell instrument to generate single-cell beads in the emulsion (GEMs). Then the scRNA-seq libraries were constructed by using the Chromium Controller and Chromium Single Cell 3Reagent Kits (v3 chemistry CG000183) and sequenced by using the sequencer Novaseq6000 (Illumina, USA). All procedures were following the manufacturers protocol.