GSM8758268: RNase_R+_293T_siNC_2; Homo sapiens; RNA-Seq
Total RNA from cells or C. elegans strains was extracted by TRIzol Reagent or TRIzol LS Reagent according to the manufacturer?s procedures. The concentration of isolated RNAs was verified by Nanodrop, and RNA quality was checked with gel electrophoresis. For ribosomal RNA-depleted (Ribo-) RNA-seq, rRNA in total RNA was depleted with the Illumina Ribo-Zero Gold kit (Illumia) and subjected to library preparation with the TruSeq Ribo Profile Library Prep Kit (Illumina) according to the manufacturer?s instructions. For Ribo-/RNase R+ RNA-seq, total RNA was rRNA-depleted, RNase R-treated and subjected to library preparation according to the manufacturer?s instructions.