GSM8457642: oeCTCFwt_IAA_rep1; Mus musculus; RNA-Seq
Total RNAs were extracted from cells with a RaPure Total RNA Micro kit. RNA sequencing libraries were constructed using the VAHTS Universal V8 RNA-seq Library Prep Kit for Illumina. Two rounds of mRNA purification were performed to guarantee the removal of rRNA. The mRNAs were fragmented to 250-450 bp at 85°C for 6 min, followed by immediate cDNA first- and second-strand synthesis, end-repair and adapter ligation reactions. cDNA purification and size selection were performed by AMPure XP beads.