RNA-Seq of horse fibroblasts derived form ovary stroma
Total RNA was extracted following the manufacturer's instructions. Prior to RNA library preparation, samples underwent an additional purification step using Agencourt RNAClean XP (Beckman Coulter, Brea, CA, USA). Libraries were then constructed using the QuantSeq 3 mRNA-Seq Library Prep Kit FWD for Illumina. Their quality and quantity were assessed using the Agilent TapeStation 2200 and Qubit Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA), respectively. After quality control and normalization, the libraries were sequenced as single reads with a read length of 2150 bp on the NovaSeq 6000 System (Illumina, USA) at the OMRF Clinical Genomics Center (CGC) in Oklahoma, USA. Only the first read was used for downstream analysis.