RNA-seq of Abies alba: leaves (pool)
Abies alba 3-year-old trees were subjected to eight different treatments (control, soft cold, extreme cold, heat, drought, darkness, growth hormones, physical damage) in control growth chambers (CGC). Total RNA was extracted from 100 mg of each sample using SpectrumTM Plant Total RNA Kit (Sigma-Aldrich, USA). Samples were then pooled by tissue (at least 1000 ng of RNA in each pool, adding the same amount of each sample): 3 different pools (3 tissues with 24 pooled samples each). mRNA libraries were constructed and sequenced at Macrogen (Korea): PacBio Iso-Seq libraries were prepared for long-read sequencing on PacBio Sequel IIe system (8 Gb/sample).