RNA-Seq of Mus musculus : PN7 Anterior cerebellum Tlx3 cKO 2
RNA Sequencing was carried out between control and Tlx3 cKO posterior and anterior cerebellum at PN7 stage and total RNA was isolated using RNeasy kit (Qiagen) following manufacturers protocol. A minimum of 100ng of isolated mRNA was used to prepare sequencing libraries with the TruSeq stranded mRNA reagent (Illumina), and sequencing was performed on a HiSeq Illumina Analyzer.