NGS analysis of Myeloid-Derived Suppressor Cells (MDSC) and Granulocytes isolated directly after major surgery
NGS analysis of Myeloid-Derived Suppressor Cells (MDSC) and Granulocytes isolated directly after major surgery
Ficoll Density gradient centrifugation and subsequent microbead separation. miRneasy (Qiagen) according to manufacturer's instructions The Nucleic acid library construction was performed by Novogene according to their instructions.