GSM8458363: Patient 14, breast tumor; Homo sapiens; RNA-Seq
Total RNA was extracted from 10 mg of snap-frozen tissue on a KingFisher Flex instrument (Thermo Scientific, 5400610) using the MagMAX mirVana total RNA isolation kit (Applied Biosystems, A27828). Nucleic acid quantification was performed on a Qubit Flex Fluorometer (Thermo Scientific, Q33327) using the Qubit RNA broad range assay (Invitrogen, Q10211), and RNA quality was evaluated on a TapeStation 4200 instrument (Agilent, G2991AA) by RNA ScreenTape Analysis (Agilent, 5067-5576). Libraries were prepared from 1 µg of total RNA on a Hamilton NGS STAR instrument using the TruSeq Stranded Total RNA Library Prep Gold Kit (Illumina, 20020599). Library quantification was performed on a Qubit 3.0 Fluorometer (Thermo Scientific, Q33216) using the Qubit 1X dsDNA broad range assay (Invitrogen, Q33266). Library quality was evaluated on a TapeStation 4200 instrument (Agilent, G2991AA) by DNA ScreenTape Analysis (Agilent, 5067-5582).