GSM8574751: myopba5; Mus musculus; RNA-Seq
RNA was extracted using the standard chloroform extraction and isopropanol precipitation method. RNA concentrations and quality were determined using a TECAN infinite F-nano+ spectrophotometer. 750 ng of Poly (A) enriched RNA was used for directional RNAseq library preparation using the NEBNext Ultra II RNA Library Prep Kit according to standard protocol.