GSM8573353: PMA-stimulated THP1 cells, 2h, vehicle (DMSO:EtOH), MED1 ChIP-seq, replicate 2; Homo sapiens; ChIP-Seq
The cells were cross-linked with 2 mM disuccinimidyl glutarate for 40 minutes and 1% methanol-free formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sheared using a Diagenode Bioruptor sonicator. Precipitated and washed samples were purified using NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel). Indexed libraries were prepared from ChIP-DNA using a TruSeq ChIP Sample Preparation Kit (Illumina).