WGS of Arthrobacter RMB11
DNA was randomly sheared using a Covaris system, and library preparation was carried out using the Agencourt AMPure XP-Medium kit to obtain fragments averaging 200400 bp in length. Fragment ends were repaired and 3-adenylated, followed by ligation with sequencing adapters. The ligated fragments were then PCR-amplified, heat-denatured, and circularized. DNA nanoballs (DNBs) were generated via rolling circle amplification using phi29 polymerase followed by sequencing on the DNBSeq-G400 platform, generating 150 bp paired-end reads.