18S metabarcoding of soil sample
Libraries were constructed using 2-stage PCR using the primers AMV4.5NF and AMDGR for PCR 1.  PCR2 added an 8 bp index sequence and Illumina adaptors.  Products were cleaned using Charm Normalization plates, the library was pooled and concentrated using KAPAPure beads, and finally quality checked using QUBIT and BioAnalyzer