16S rRNA V3-V4 sequencing of infant gut microbiome
Amplicon sequencing of V3-V4 hypervariable regions of the 16S rRNA gene from fecal samples of 4-month-old infants. Library preparation involved PCR amplification using primers targeting the V3-V4 regions, followed by paired-end sequencing on the Illumina MiSeq platform (300 bp reads). Quality control and taxonomic classification were performed using QIIME2 and DADA2 pipelines with the Silva 138 database.