WGS of Rhodococcus opacus IEGM 249: DNA
Quick-DNA Fungal/Bacterial Microprep Kit/Quick-DNA Fecal/Soil Microbe Microprep kit (Zymo Research) to extract DNA. Illumina DNA Prep to produce paired-end library [2  100 nucleotides (nt)]. Qubit 4 to quantify DNA. Electrophoresis in 1% agarose gel to qualify DNA.