GSM8655182: wildtype GV oocyte RNA-seq biol rep2; Mus musculus; RNA-Seq
For RNA-seq of MatKO and control oocytes RNA from ~10 pooled mouse oocytes per replicate was extracted with the Arcturus PicoPure RNA Isolation Kit (Thermo Fisher Scientific). cDNA libraries were generated from total RNA using the SMARTer Ultra Low Input RNA Kit for sequencing (Clontech), followed by sequencing library preparation with the NexteraXT DNA Sample Preparation Kit (Illumina) according to manufacturer's instructions. Final libraries were amplified and pooled for single-end Illumina sequencing.