GSM9682545: JFS3030_M07e_FHD286_48hr_H3K27ac_rep1; Homo sapiens; OTHER
Cells were harvested, washed in cold PBS, and nuclei were isolated according to the AutoCUT&RUN protocol. Nuclei were incubated overnight at 4°C with antibodies targeting mSWI/SNF subunits (SMARCA4), CBFA2T3::GLIS2 fusion components (CBFA2T3, GLIS2), or histone modifications (H3K27ac, H3K4me3), or IgG isotype control. Following pA-MNase incubation, chromatin cleavage was initiated by CaCl₂ addition at 0°C, and released DNA fragments were purified. CUT&RUN libraries were prepared at the Fred Hutchinson Cancer Center Genomics Core using the AutoCUT&RUN protocol. Paired-end sequencing was performed on an Illumina instrument.