Hi-C of Hippophae rhamnoides: variety Triumf
Nuclei were isolated from leaves fixed with formaldehyde. Then, restriction with DpnII, biotin labeling with Biotin-15-dCTP, ligation of DNA fragments with T4 DNA ligase, and DNA isolation were performed. The library was prepared using the Qiaseq FX DNA kit including the step of enrichment of the biotinylated fragments.