GSM8573441: PEO1 A, ATAC, Rep2; Homo sapiens; ATAC-seq
Cells were harvested and lysed to obtain nuclei. The nuclei then underwent a transposition reacrion using Tn5, and the DNA was purified after using Qiagen MinElute PCR Purification Kit. Libraries were made using Custom Nextera PCR Primers containing barcodes, and amplified 8-10 times on a thermocycler depending on results of a qPCR to ensure minimal amplification. Library quantification and frgament distribution were analysed using an Agilent 4200 TapeStation system using the Agilent high sensitivity D1000 screentape following manufacture's instructions.