Tiled RT-PCR of SARS-CoV-2 from wastewater.
Total nucleic acid from wastewater solids treated with TurboDNase, concentrated, reverse transcribed with LunaScript, amplified with ARTIC v5.3.2 primers using NEB Q5, converted into Illumina-compatible libraries using NEB Ultra II DNA kit, and sequenced on an Illumina system 300 PE (XLEAP-SBS) or 250 PE (standard SBS).