RNA-seq of Physella sp: foot
Animals were anesthetized, and the posterior part of the foot was quickly severed and immediately stored in Zymo DNA/RNA Shield. Total RNA was isolated using the Lexogen SPLIT RNA Extraction Kit and treated with DNase. For each sample, RNA from the foot tissue of three individuals was pooled in equimolar amounts. Libraries were prepared using the Lexogen CORALL mRNA-Seq V2 kit (RTL protocol), and sequencing was performed in PE-150 mode on the Illumina NovaSeq X Plus.