GSM9022734: NSAIDS-XDR-TB-CT-13-M6; Homo sapiens; RNA-Seq
Total RNA was extracted from whole blood samples using the PAXgene Blood miRNA Kit (PreAnalitiX, Hombrechtikon, Switzerland) procedure, according to the manufacturer's protocol. Total RNA quality was quantified using the Quant-iT Broad-Range dsDNA Assay Kit (Thermo Fisher Scientific), and RNA integrity was assessed using the Fragment Analyzer system HS RNA Kit (15NT) (Agilent). RNA-Seq libraries were prepared with the Illumina Stranded Total RNA Prep with Ribo-Zero Plus (Illumina), following the manufacturer's recommendations, starting with 0·5 μg of total RNA as the input material. The final library was validated on an Agilent 2100 Bioanalyzer using the DNA 7500 assay. The libraries were sequenced on the NovaSeq 6000 (Illumina) with a read length of 2x101 bp following the manufacturer's protocol for dual indexing.