GSM9312222: Su113 NC14, RNA-seq, rep2; Drosophila melanogaster; RNA-Seq
Cages of flies with apple juice agar plates were set up and maintained at 25°C for 24-48h. Flies were allowed to lay eggs for one hour, and embryos were aged for another two hours at 25°C.  NC14 Hand-sorted embryos were flash-frozen and stored in batches of 50 per sample. libraries were prepared using the Illumina TruSeq Stranded Total RNA kit, following the manufacturer's instructions.