GSM9679227: NPM1_2; Mus musculus; OTHER
Pachytene spermatocytes were isolated from mouse testes (4-6 weeks old) by FACS. Cells were crosslinked with 1% formaldehyde for 10 min, quenched with glycine, and lysed. Soluble chromatin was collected by centrifugation and used for CUT&Tag. CUT&TAG enriched DNA were used for library preparation using theHyperactive Universal CUT&Tag Assay Kit for Illumina Pro following the manufacturer's protocol. Libraries were amplified by 12-15 cycles of PCR, purified with VAHTS DNA Clean Beads. Quantification was performed by Qubit, followed by pooling for sequencing.