LARP4_RIP-seq (CRX583054; CRA009783)
RNA immunoprecipitation (RIP) was performed with an EZ-Magna RIP Kit.Briefly, First, 2 X 107 naive CD4+ T cells were lysed by RIP lysis buffer and frozen overnight at -80degreeC. Then, immunoprecipitation (IP) was performed using anti-LARP4 antibodies, and the antibody-enriched RNAs were extracted and purified after digestion of DNA and proteins. These enriched and purified RNAs and input RNA were subject to analysis by high-throughput sequencing (DIATRE Technology Ltd). submitted_instrument_model: Illumina NovaSeq 5000