MVCO Trojan Horse amplicon series
The V4 region of the 18S ribosomal RNA gene was amplified from DNA isolated from seawater collected at MVCO.  The primers used for amplification are from Hugerth et al 2014 (PLOS One).  Primers were modified to carry 5' adapter sequences specified by URI Genomics and Sequencing Center.  AmpliTaq Gold 360 was used for amplification, and triplicate reactions were accomplished for each sample.  Replicates were combined and purified using Agencourt AMPure XP.  Products were sent to URI Genomics and Sequencing Center for Illumina MiSeq.