GSM9644297: cMoP H3K4me2 CUTAC DE_1; Mus musculus; OTHER
CUTAC was performed using the CUTANA CUT&Tag Kit (EpiCypher, Version 4) following the manufacturer's protocol with a no-salt tagmentation condition. A total of 1 × 105 cells were used per sample. Primary antibodies used were H3K4me3 and Rabbit IgG (included in the kit), and H3K4me2 (EpiCypher 13-0027). A secondary anti-Rabbit IgG (ABIN 101961) antibody was used. Libraries were prepared using the CUTANA CUT&Tag Kit (EpiCypher, Version 4) following the manufacturer's protocol. Indexed libraries were pooled and sequenced as 150-bp paired-end reads on an Illumina NovaSeq X Plus platform.