RNA-seq of human endometrial tissue
RNA-seq of human endometrial tissue
Endometriotic tissues as well as samples of healthy endometrium were harvested laparoscopically at the time of the operation and immediately flash frozen in liquid nitrogen and stored in deep freeze until processing.  Frozen tissues were grinded with mortar and pestle and the total RNA was isolated with Trizol reagent following manufacturer's protocol. 1Âµg of DNase I-treated RNA isolated from tissues was used for poly-A mRNA isolation with the NEBNext Poly(A) mRNA Magnetic Isolation Module. Paired-end libraries were prepared using the NEBNext Ultra II Directional RNA Library Kit with NEBNext multiplex index oligos for Illumina (Dual Index Primers Set 1) (New England Biolabs).