Soil microbial community from the tropical dry forest Lowland Deciduous Forest and Shrubland of Jama-Zapotillo (LDF), Ecuador, during the rainy season, plot P3, transect T3
Ribosomal V4 region amplified with 515F-Y (Parada et al., 2016) and 926R (Quince et al., 2011), and the Illumina adapter overhang in both primers for a dual-barcoding approach. Amplification using PCRBIO HiFi Polymerase (PCR Biosystems Ltd) under the following conditions: 95C for 3 min, followed by 28 cycles of 95C for 20 s, 60C for 20 s and 72C for 30 s, and final elongation step at 72C for 5 min. PCR products were purified with Agencourt AMPure XP Beads (Beckman Coulter Inc.), and libraries prepared with the Nextera XT DNA Library Preparation Kit (Illumina Inc.).