RNA-seq of tomato M82 leaf in drought, replicate 1
RNA was extracted from terminal leaflets of the third fully expanded leaf of 5-week-old Solanum lycopersicum plants (M82 and tiny1-23 mutant) grown under irrigated and drought conditions. The experiment included 12 biological samples: three replicates each for the two genotypes under both irrigation regimes. RNA-seq libraries were prepared using the INCPM-mRNA-seq protocol, based on poly(A) selection, and sequenced on the Illumina NextSeq platform to generate 75 bp single-end reads. The design aimed to identify TINY1-dependent transcriptional responses to drought and developmental regulation.