GSM9644159: MPJ_BM2; Mus musculus; RNA-Seq
kidneys, spleens, tibiae, and femurs were harvested into 10% FBS-supplemented RPMI 1640. The kidneys were enzymatically digested with collagenase D (Sigma Aldrich) in RPMI 1640 for 30 minutes using a gentleMACS Dissociator (Miltenyi Biotec) and then washed with 10% FBS-supplemented RPMI 1640. The BM cells were isolated via centrifugation, while splenocytes were obtained through mechanical dissociation. RBCs in all samples were lysed with RBC lysis buffer (BioLegend), and cellularity was determined using a Cellometer Automatic 2000 (Nexcelom Bioscience). A chromium single-cell library was constructed using the Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (Dual Index, 10x Genomics). Sequencing was performed on a NovaSeq 6000 (Illumina) with 2 × 150-bp, 20,000 150-bp paired-end reads, yielding reads captured cell.