DNA sequence of Trypanosoma brucei brucei
DNA was extracted from human blood using a QIAGEN DNeasy blood and tissue DNA Kit (250preps), PCR performed with generic primers to amplify the ITS1 region of the Trypanosoma spp. Bands below 500bp were subcloned into  pJET1.2/blunt plasmid and inserted into the XL-Blue E. Positive colonies were checked and purified for sequencing. The libraries were constructed by the Sequencing company