RNAseq of small intestinal organoids prepared from wild-type and mutant mice that harbour a point mutation that converts a serine residue at position 839 to an isoleucine
RNAseq of small intestinal organoids prepared from wild-type and mutant mice that harbour a point mutation that converts a serine residue at position 839 to an isoleucine
Small intestine was isolated from mice for organoid preparation. Preparation was as per protocols published by StemCell Organoids prepared from wild type and S839I mice were cultured for 3 days after passaging and then treated with interferon gamma or uroguanylin or both together for 16h. Total RNA purified form cultured organoids using Invitrogen RNA purification kits. RNA was treated with DNAse on the column. mRNA fragment and synthesis of cDNA followed by end repair and dA tailing. cDNA purified and amplified by PCR