GSM8967587: H3K27ac ChIP-seq in dTAG13-treated (24hr) SEM cells with MYB endogenously tagged with FKBP12(F36V); Homo sapiens; ChIP-Seq
Cells were washed with PBS and fixed in either 1% FA for 10 minutes (histones) or 2mM DSG for 30 minutes and 1% FA for 30 minutes (TFs). Cells were sonicated to give fragments of 100-300bp followed by IP. DNA libraries were made using the NEBnext Ultra DNA II library preparation kit for Illumina (Cat no. E7645).