GSM8966712: PC3_27_HIF1bKOclone13_Nx_H3; Homo sapiens; ChIP-Seq
Chromatin immunoprecipitation (ChIP) experiments were performed using anti-H3K4me3 (Cell Signaling, 9751S), anti-H3K9me3 (Cell Signaling, 13969S), anti-H3K27me3 (Cell Signaling, 9733S), anti-H3K36me3 (Cell Signaling, 4909S), anti-H3 (Abcam, ab1791) antibodies. In addition, 10ng of Drosophila melanogaster chromatin (Active Motif, 53083) and 1µg of Drosophila-specific histone variant H2Av antibody (Active Motif, 61686) were added to each reaction as a spike-in control for downstream normalisation. All libraries were prepared and sequenced by the US NCI CCR Genomics Core.