16S rRNA gene amplicon data from wastewater microbiome
DNA was extracted by FastDNA SPIN Kit for Soil. Bacterial 16S rRNA (variable regions 1-8 (bV18-A) amplicon libraries were prepared and sequenced using Nanopore technology. The forward and reverse primers used include custom 24 nt barcode sequences followed by the sequences targeting bV18-A: [8F] AGRGTTYGATYMTGGCTCAG and [1391R] GACGGGCGGTGWGTRCA. The resulting amplicon libraries were purified using the CleanNGS SPRI beads. Sequencing libraries were prepared from the purified amplicon libraries using the SQK-LSK114 kit (Oxford Nanopore Technologies, UK). The resulting sequencing library was loaded onto a PromethION R10.4.1 flowcell and sequenced using the MinKNOW 24.02.10 software (Oxford Nanopore Technologies, UK).