RNA-seq of WT3A P. berghei anka
Total RNA was extracted using TRIzol reagent (Invitrogen) according to the manufacturers instructions. RNA quality was assessed using an Agilent 2100 Bioanalyzer. mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and fragmented into short fragments. First-strand cDNA was synthesized using random hexamer primers and reverse transcriptase, followed by second-strand cDNA synthesis. After end repair, A-tailing, and adaptor ligation, the products were PCR amplified and purified to obtain the final sequencing libraries. The libraries were quantified and sequenced on an MGISEQ-2000 platform to generate paired-end reads.