Whole genome sequencing of Zika virus isolate R103451 using Illumina MiSeq
Viral RNA extracted from infected Vero cell culture supernatant. cDNA synthesized using random hexamer priming followed by Illumina paired-end library preparation. Sequenced on Illumina MiSeq platform generating 2 x 150 bp reads. Adapter trimming and quality filtering performed using fastp v1.1.0 prior to downstream analysis.