16S rRNA Amplicon Sequencing of human gut metagenome: Pooled gut microbiota samples of Turkish pediatric ADHD patients and healthy controls
16S rRNA gene V5-V7 region amplicons were generated from gut DNA samples and purified using Qiaseq beads Clean-Up Kit (Qiagen). Sequencing libraries were prepared using the Nextera XT DNA Library Prep Kit (Illumina) with multiplexing index assignments performed via the TG Nextera XT Index Kit v2 Set A (Illumina). Library concentrations were verified using the iQuant dsDNA HS Assay Kit (ABP Bioscience). High-throughput paired-end (2x150 bp) sequencing was executed on the Illumina iSeq100 platform.