16S of Daphnia microbiome
Raw data files in FASTQ format were subject to reads merge by overlapping sequences, data quality control, and chimera filtering, resulting in high-quality clean data. Next, Sequence noise reduction was performed using QIIME2 D2DA2 based on CleanData, possible PCR amplification and sequencing errors in high-throughput sequencing data were removed, and the abundance tables of representative correct biological sequences ASVs (Amplicon Sequence Variants) and ASV were obtained. Then, subsequent statistical analyses including alpha diversity, beta diversity, taxonomic composition, function prediction and difference analysis were performed.