RNA Seq of malabar mahogany
Total RNA was extracted from the received plant samples by using SpectrumTM Plant Total RNA kit (Sigma-Aldrich). Quality and quantity of the extracted RNA samples were checked on NanoDrop followed by Agilent Tape station using High Sensitivity RNA ScreenTape. The QC passed RNA samples were processed for PE library preparation using Illumina TruSeq stranded mRNA Library Prep kit as per the kit protocol. The quality of all the prepared libraries passed in our QC step processed for sequencing on Illumina Novaseq 6000 platform using 2 x 150 bp chemistry.