RNAseq of human endometrial monocytes and macrophages in healthy controls and pre-eclampsia
RNAseq of human endometrial monocytes and macrophages in healthy controls and pre-eclampsia
Endometrial pipelle biopsies were digested in collagenase 8, collagenase D and dispase for 35 minutes prior to antibody staining for FACS enrichment of monocytes, Mac1 macrophages and Mac2 macrophages. Samples collected in RLT buffer and RNA isolated using Qiagen RNeasy Plus Micro kit.  Library preparation of samples was performed using the Illumina TruSeq Stranded mRNA Sample Preparation Kits (Illumina).