RNAseqsamplesforID3491
Sorted single-cell suspensions prepared from each sample were washed twice with 0.05% UltraPure BSA (Invitrogen #AM2618) in PBS. For PBMC samples, 9,000 cells were loaded into a 10x Chromium controller instrument along with Chromium Next GEM Single Cell 5' beads (10x Genomics PN-1000263). Up to four PBMC samples were multiplexed together after being tagged with unique DNA-barcoded antibodies as described above.