GSM9137468: 4 Gy Irradiated, organoids, 11 days, mouse 2; Mus musculus; RNA-Seq
For the cDNA library preparation, total RNA was extracted from organoids using Rneasy Mini Kit according to the manufacturer`s instructions and its quality was determined using High Sensitivity RNA ScreenTapes on an Agilent 2100 Bioanalyzer. 100 ng of RNA was used as an input for all samples. 80 ng of highly intact RNA (RIN values > 9) was processed for the cDNA library preparation using the Smart3SEQ protocol, adhering to the manufacturer`s instructions.