GSM8967417: Neutral Rev Pol2; Mus musculus; ChIP-Seq
Cells were fixed on plate in 1% formaldehyde (in PBS) at room temperature for 10 mins with agitation. After quenching and cell lysis, chromatin was sheared using E220 Focused Ultrasonicator (Covaris) for 10 minutes. Libraries were prepared using the NEBNext Ultra II DNA kit (NEB, E7645S) using up to 50 ng of input or ChIP DNA, with ~4-8 cycles of amplification, with no pre-PCR size selection but a post-PCR double-sided 0.5x/0.9x Ampure XP bead clean-up. Final quantification and QC were assessed by Qubit(Thermo) and Tapestation (Agilent), respectively, and libraries were pooled for sequencing on NovaSeq X Plus (Illumina) platform