GSM8510333: hiPSC_NPCs_rep2; Homo sapiens; RNA-Seq
Total RNA was extracted from hiPSC-NPCs and hiPSC-FS-NPCs using the RNeasy Mini Kit (Qiagen), and the quality of the RNA was assessed using a Bioanalyzer (Agilent) to ensure that the RNA integrity number (RIN) was above 7.0. After poly(A)+ enrichment using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England BioLabs), double-stranded cDNA libraries were prepared using the SMARTer Stranded Total RNA Sample Prep Kit-HI Mammalian (Clontech).