GSM9680389: Day 30 human retinal organoids scRNA-seq; Homo sapiens; RNA-Seq
Day 30 human retinal organoids were dissociated into single-cell suspensions using enzymatic digestion with TrypLE Express (Thermo Fisher Scientific) combined with gentle mechanical trituration. Cell suspensions were filtered to remove debris and assessed for viability using trypan blue exclusion. Only samples with >85% viable cells were used for downstream library preparation. Single-cell RNA sequencing libraries were prepared using the Chromium Single Cell 3′ Reagent Kit v3 (10x Genomics) according to the manufacturer's instructions. Approximately 8,000 cells were loaded onto a Chromium microfluidic chip to generate single-cell gel beads-in-emulsion (GEMs). Following reverse transcription, cDNA amplification and library construction were performed using standard protocols. Libraries were sequenced on an Illumina NovaSeq 6000 platform with paired-end 150 bp reads.