GSM8710046: PBMC sorted cell TEMRA, Mini, sublibrary 8; Homo sapiens; RNA-Seq
cell suspensions were filtered through a 40 µm flowmi cell strainer (SP BEL-ART) and the cell count, and viability were determined using 0.4% trypan blue dye (Gibco). Live PBMCs (viability > 95%) were then resuspended with 1 mL 1X PBS containing 1% UltraPure bovine serum albumin (Thermo Fisher Scientific). The cells were loaded onto the Chip G and Chromium Controller instrument (10x Genomics) to generate gel bead-in-emulsions (GEM) and indexed sequencing libraries according to the Chromium Next GEM Single Cell 3' Reagent Kits v3.1 user guide.