GSM8995499: Whole testis, KO RAD21L, scRNAseq; Mus musculus; RNA-Seq
Testicular disaggregation from 55 days post-partum Rad21l+/+ and Rad21l-/- mice was conducted as previously described (1). An aliquot of the single-cell solution was loaded into a ChromiumTM Single Cell Chip (10x Genomics®), aiming to recover between 5,000 and 6,000 cells. Libraries were prepared following manufacturer's instructions and sequenced on Illumina NovaSeq 6000 (50bp pair ends).