Whole genome sequencing of sample I0003206-5
DNA from sputum samples of TB patients was extracted from cryopreserved cultures. Each isolate was thawed and subcultured on LowensteinJensen medium and a big loop of colonies were lysed with lysozyme and proteinase K to obtain DNA using CTAB/Chloroform extraction and ethanol precipitation. DNA was sheared into ~ 250 bp fragments using a Covaris sonicator (Covaris,Inc.), and prepared using the TruSeq Whole-Genome Sequencing DNA sample preparation kit (Illumina, Inc.). Samples were sequenced on an Illumina HiSeq 2500 sequencer. Paired-end reads of length 125 bp were collected.