Vyarna Bio Information Booster 1 Positive
The 16S rRNA V1V3 region was amplified using the Quick-16S NGS Library Prep Kit (Zymo Research, Irvine, CA) with real-time PCR to control amplification cycles and minimize chimera formation. Libraries were quantified (Qubit, TapeStation), purified (Select-a-Size DNA Clean & Concentrator), and sequenced on an Illumina MiSeq (2300 bp, P1 kit) with a 10% PhiX spike-in