GSM8758532: A; Homo sapiens; ATAC-seq
Lysates were clarified from sonicated nuclei and pan-Kac-DNA complexes were isolated with antibody. Libraries were prepared according to the TruePrep® DNA Library Prep Kit V2 for Illumina (TD501-01). After adapter ligation DNA was PCR amplified with Illumina primers for 25 cycles and library fragments of ~250 bp (insert plus adaptor and PCR primer sequences) were band isolated from an agarose gel. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Illumina HiSeq 2500 following the manufacturer's protocols.