Total RNA miR-seq : WT1
Small RNA libraries for miR-seq were generated using the QIAseq miRNA Library Kit (Qiagen, 331502) according to the manufacturer's instructions. For normalization of total RNA samples, 1ul of QIAseq miRNA Library QC Spike-ins (Qiagen, 331535) was added to 500 ng of RNA before library preparation. The library was size-selected and purified on a native 6% (w/v) acrylamide gel and ethanol precipitation, respectively. The library was sequenced on the Illumina Nextseq 1000 platform.