GSM9350916: Amygdala_snRNAseq_R848_35; Mus musculus; RNA-Seq
Brains were extracted and flash frozen in isopentane. dmPFC and AMG were cryosectioned and nuclei were isolated with the Chromium Nuclei Isolation Kit following manufacturer's protocol with lysis time reduced to 4.5 min (10X Genomics) libraries were prepared utilizing the Chromium Next GEM Single Cell 3' Kit v3.1 (10X Genomics), and sequenced on a NovaSeq 6000 using an S4 flow cell at the Genomic Sequencing and Analysis Core Facility at UT Austin