WGS of Human: adult male buffy coat
DNA was isolated from buffy coat using QIAamp DNA Mini Kit. The gDNA purity, quantity and size distribution was determined with Qubit (Invitrogen) and TapeStation 4200 (Agilent) analysis prior to DNA-seq library preparation. An input of 55ng of the gDNA was mechanically sheered (Covaris) targeting 300 - 400bp DNA products and the Ovation Ultralow System V2 kit (Tecan) was used to generate DNA-Seq libraries. Paired-end sequencing reads of 150bp was generated on NovaSeq 6000 (Illumina) sequencer at a target depth of 600 million paired-end reads per sample. Raw sequencing reads were de-multiplexed using bcl2fastq. WGS analysis was performed using Integrated Genome Viewer (IGV).