GSM8572793: BR1058 SEB Y1 Flu Day 0; Homo sapiens; RNA-Seq
We extracted RNA using the MagMAX RNA Isolation Kit (Thermo Fisher Scientific) then performed globin reduction with the GLOBINclear-Human Kit (Thermo Fisher Scientific) We generated full-length cDNA from total RNA (0.5 ng). We amplified cDNA using the SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio) and constructed sequencing libraries using the NexteraXT DNA Library prep kit (Illumina). We pooled libraries, quantified concentration with a Qubit Fluorometer (Life Technologies), then sequenced on a NextSeq 2000 sequencer (Illumina) with 59-base paired-end reads on a NextSeq P2 flow cell (Illumina). We targeted a depth of 5 million reads per sample.