GSM9739734: Jejunum-Khk KO-3; Mus musculus; RNA-Seq
RNA was extracted using Qiagen Rneasy Mini Kit Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library.