PacBio HiFi sequencing data from Odocoileus hemionus
The HiFi SMRTbell library was constructed using the SMRTbell prep kit 3.0 (Pacific Biosciences, Menlo Park, CA; Cat. #102-182-700) according to the manufacturer's instructions. HMW gDNA was sheared to a target DNA size distribution between 15-18 kb using Diagenodes Megaruptor 3 system (Diagenode, Belgium; cat. B06010003). The sheared gDNA was concentrated using 1X of SMRTbell cleanup beads provided in the SMRTbell prep kit 3.0 for the repair and a-tailing incubation at 37C for 30 minutes and 65C for 5 minutes, followed by ligation of overhang adapters at 20C for 30 minutes, clean-up using 1X SMRTbell cleanup beads, and nuclease treatment at 37C for 15 minutes. The SMRTbell library was size selected using 3.1X of 35% v/v diluted AMPure PB beads (Pacific Biosciences, Menlo Park, CA; Cat. #100-265-900) to progressively remove SMRTbell templates <5kb.  The 15  18 kb average HiFi SMRTbell library was sequenced at UC Davis DNA Technologies Core (Davis, CA) using three 8M SMRT cell (Pacific Biosciences, Menlo Park, CA; Cat #101-389-001), Sequel II sequencing chemistry 2.0, and 30-hour movies each on a PacBio Sequel IIe sequencer.