RNA-Seq of homo sapiens: adult female skin
Total RNA was extracted using trizol method. All the RNA samples were assessed for their integrity using Qsep400 instrument. To construct RNA libraries with the VAHTS mRNA-seq V8 Library Prep Kit for Illumina, 1 g of total RNA was used. The procedure included polyA-selected RNA extraction, RNA fragmentation, random hexamer primed reverse transcription, and 150nt paired-end sequencing by Illumina Novaseq 6000.