Next generation sequencing data
Treated cells or tissues were collected after four days and genomic DNA was isolated. On-target and off-target genomic regions of interest were amplified by PCR with flanking primers (Forward: CCTCTCAAGGCTGTGCAGATGCT; Reverse: CCACGAAGAGCAGGGTGAAGATGA). Samples were sequenced and analyzed to detect CRISPR variants from NGS reads.