16S amplicon of saline lake bacterioplankton
The extracted DNA was subjected to PCR using primers (Klindworth et al., 2013) targeting the V3V4 hypervariable regions of the 16S rRNA gene (S-DBact-0341-b-S-17 = CCTACGGGNGGCWGCAG; S-D-Bact-0785-a-A-21 = GACTACHVGGGTATCTAATCC).