GSM9427889: Adnp, WT, EFS-TRAPed, 4 month, male, replicate2; Mus musculus; RNA-Seq
Prefrontal cortex (PFC) tissue was dissected and pooled (three mice per biological replicate). Tissue was homogenized on ice for nuclei extraction using a detergent-based lysis buffer, followed by filtration and centrifugation. Isolated nuclei were washed, counted, and immediately used for downstream processing or stored at -80°C. Total nuclear RNA was extracted using a column-based RNA purification kit with on-column DNase treatment, according to the manufacturer's instructions. Single-nucleus RNA-seq libraries were generated using the 10x Genomics Chromium Single Cell 3' workflow.