stem_2 (CRX2061358; CRA031216)
The total RNA of the sample was extracted using a Trizol reagent kit (Invitrogen, Carlsbad, CA, USA). The purity, concentration and integrity of the RNA samples were assessed. The cDNA libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #7530, New England Biolabs, Ipswich, MA, USA). Finally, the cDNA library was sequenced using the Illumina Novaseq 6000 platform in the paired-end mode by Genepioneer  Co., Ltd (Nanjing, China).