Root-85-24h-3
Sequencing libraries were generated using DNBseq technology. DNBseq technology consists of DNA NanoBalls (DNBs) which are small, high-density sequencing templates. Rolling-circle replication (RCR) linear amplification makes copies of the original DNA template, instead of copies-of-a-copy. Combinatorial Probe-Anchor Synthesis (cPAS) sequencing chemistry incorporates a fluorescent probe to a DNA anchor on the DNB, followed by high-resolution digital imaging.