Cut Tag of HCT116 cells
CUT&Tag was performed using the commercially available CUT&Tag kit from Novoprotein Corporation (Cat. #N259-YH01). Briefly, HCT116 cells expressing HMOX1-FLAG were enzymatically dissociated, and cells (>90% viability by trypan blue exclusion) were processed following the manufacturers standard protocol. Cells were incubated with anti-FLAG primary antibody and protein A-Tn5 transposase. After tagmentation, DNA was extracted and libraries were amplified with indexed primers, purified, and sequenced.