GSM8457908: AE9a mBM c-Kit+ cells, DMSO-3; Mus musculus; RNA-Seq
A total of 300 c-Kit+ cells per group were sorted using FACS and lysed directly with Smart-seq2 lysis buffer (Vazyme, Cat. TD502). The RNA from the sorted cells was reverse-transcribed and amplified following the SMART-Seq2 protocol to generate sufficient cDNA. After quantifying the cDNA, 5 ng of cDNA was utilized to construct the cDNA library using the TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme, Cat. TD502). SMART-seq2