Stool sample of allo-HCT patient shotgun sequenced
After PicoGreen quantitation and quality control by Agilent TapeStation, sequencing libraries were prepared from amplicons with an average size of 451 bp with an input of 100 ng using the KAPA HTP Library Preparation Kit (Roche catalog # 07961901001) according to the manufacturers instructions with 8 cycles of PCR. Barcoded libraries were pooled equimolar and run PE250 on a MiSeq using the TruSeq SBS Kit v3 (600 Cycles). The average number of read pairs per sample was 45 million.