Protein sequencing library generated with the MissionBio Tapesti platform from a primary AML sample, using DNA oligo-conjugated antibodies against 42 unique cell surface antigens.
Single cell mutation analysis was performed on patient AML samples (blood or marrow mononuclear cells) to document the subclonal composition. We utilized the Tapestri Single Cell DNA plus Protein Sequencing Kit (PN6870 A1, MissionBio) and the Tapestri Single-cell DNA Myeloid Panel (MissionBio) for targeted sequencing. The protein library was obtained by utilizing the TotalSeq™-D Human Heme Oncology Cocktail, V1.0 (Biolegend Cat No 399906) consisting of antibodies for 42 surface antigens as part of the protocol on the Tapestri instrument. The phenotype was analyzed using Mosaic software (MissionBio).