GSM8510320: AML_iPSC_Rep3, RNA-seq; Homo sapiens; RNA-Seq
RNA was extracted from cells after drug treatments using AllPrep DNA/RNA Mini Kit (Qiagen), RNA from iPSCs were also collected (day 0). Illumina stranded mRNA prep ligation sample preparation protocol was used with starting concentration between 25-1000 ng total RNA. The protocol includes mRNA isolation, cDNA synthesis, ligation of adapters and amplification of indexed libraries. The yield and quality of the amplified libraries was analyzed using Qubit by Thermo Fisher and quality was checked by using Agilent Tapestation. The indexed cDNA libraries were normalized and combined, and the pools were sequenced on the Illumina Nextseq 2000 P2 100 cycle sequencing run, generating 58 base paired end reads with dual index.