GSM9429410: Phthalate-injected GV oocyte, Rep2; Mus musculus; RNA-Seq
For each experimental group, a single GV oocyte were transferred into lysis buffer supplemented with RNase inhibitor to preserve RNA integrity. Reverse transcription was initiated using an oligo(dT) primer containing a 5′ anchor sequence, and a template-switching oligonucleotide (TSO) enabled generation of full-length cDNA. The synthesized cDNA was subsequently amplified by PCR and purified with magnetic beads. The concentration of amplified cDNA was measured using the Qubit dsDNA HS Assay Kit, and size distribution of fragments was determined using an Agilent 4200 TapeStation system. Libraries were constructed using Nextera XT DNA Library Preparation Kit (Illumina) according to the manufacturer's guidelines and sequenced on the Illumina MiSeq platform with 150 bp paired-end (PE150) setting.