Human gut metagenome
stool samples were used for DNA isolation as per the manufacturers instructions (ZymoBIOMICS DNA Miniprep Kit, Zymo Research, CA, USA). DNA samples were validated for quantity (Nanodrop) and integrity (Agarose Gel electrophoresis) prior to shipment to a service provider (Beijing Genomics Institute, HongKong) for library preparation with magnetic beads and shotgun metagenomics sequencing on Illumina HiSeq PE150-Xten at a 30X depth