GSM8654851: p00_rbmcsatb2_bc817; Mus musculus; ChIP-Seq
P0 wild type cortices were dissected, fixed for 10 min with 1% formaldehyde and neutralized with glycine. The cells were lysed, and the chromatin was sheared into 100∼300 bp fragments. Immunoprecipitation reactions were performed using the Satb2 antibodies ChIPed DNA and input DNA were used. DNA libraries were constructed using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645, E7103) with index primers (NEB, E7335L).