RNA-Seq of Chortoglyphus arcuatus
Total RNA is extracted and mRNA is enriched using oligo dT beads. The mRNA is fragmented and converted into cDNA, with the second strand synthesized using dUTP. The cDNA fragments are end-repaired, adenylated, and ligated to adaptors. After PCR amplification and quality control, the library is circularized and amplified to form DNA nanoballs (DNBs), which are then sequenced using the DNBSEQ platform.