GSM8655337: Streptavidin_pulldown_rep1; Mus musculus; RIP-Seq
After lysis and Dnase and Rnase treatment, biotinated proteins together with RNAs were pulled down by streptavidin conjugated Dynabeads. After ligation, RNA was purified by phenol/choloform extraction. 3' and 5' RNA linkers were added to the RNA by direct ligation, followed by RT-PCR to amplify the cDNA and add indexes.