GSM8574837: neonatal mouse organ of Corti, WT, Input; Mus musculus; ChIP-Seq
Twenty OCs from WT and Bmi1-/- neonatal mice were separately harvested and sonicated to shear crosslinked chromatin. The chromatin extract was then incubated with anti-H3K27me3 (Abcam) overnight at 4°C followed by the addition of Protein A/G Dynabeads (1:1, Invitrogen). Immunoprecipitated complex was eluted from the beads and de-crosslinked, and chromatin was purified using the MinElute PCR Purification Kit (Qiagen). Purified DNA was used to prepare ChIP-seq DNA libraries for Illumina sequencing.