GSM8827637: non-POD1; Homo sapiens; RNA-Seq
Nuclei were extracted from frozen brain tissues.Frozen tissue was homogenized in a chilled lysis buffer using a Dounce homogenizer, followed by differential centrifugation with iodixanol gradient separation to isolate the nuclei. The nuclei pellet was then carefully collected, resuspended, and subjected to additional washes and centrifugation steps. The final nuclei were resuspended in PBS/1% BSA, snap-frozen, and stored 10,000 nuclei were applied for library preparation using Chromium Next GEM Single Cell Multiome Reagent Kit (10x Genomics) following the manufacturer's protocol