27S-Seq of aging mouse
For 16S rRNA gene sequencing analysis, the total DNA of flies (;100 mg) was extracted and a 16S rRNA gene amplicon sequence library was prepared as described in the protocol for 16S metagenomic sequencing library preparation (Illumina, USA). The V3-V4 region of 16S rRNA gene was amplified using a KAPA HiFi HotStart PCR kit (KAPA Biosystems, USA). Paired-end sequencing (2_x0001_ 300 bp) was performed using an Illumina MiSeq platform from Shanghai Majorbio Bio_x0002_pharm Technology Co., Ltd.