RNA-seq with and without siRNA ORF1 in post-mitotic human dopaminergic neurons (LUHMES cell line, differentiated)
RNA-seq with and without siRNA ORF1 in post-mitotic human dopaminergic neurons (LUHMES cell line, differentiated)
LUHMES cells were differentiated for 7 days. Cells were transfected with siRNA at day 3 and retrieved at day 7 of differentiation (siRNA Control or siRNA ORF1). Lund human mesencephalic cells (LUHMES) cells were differentiated for 7 days, using  Advanced DMEM/F12 added with 1% N-2 supplement and GlutaMax, supplemented with 1 µg/ml doxycycline (Sigma), 2 ng/ml recombinant human GDNF (Peprotech, # 450-10) and 1mM cAMP (Sigma, # D0627). Cells were transfected with siRNA at day 3 and retrieved at day 7 of differentiation (siRNA Control or siRNA ORF1) RNA was harvested using Direct-zol™ RNA Microprep (Zymo, # R2062). Libraries including rRNA removal were generated using the RNA Library Prep Kit for Illumina following manufacturer's recommendations.