GSM8654528: heart, surgery, Rat_61; Rattus norvegicus; RNA-Seq
The protocol was performed according to the manufacture instructions using the kit RNeasy FFPE (Qiagen, 73504). Tissues blocks deriving from the middle region were cut into sections of 20 µM and collected in microtubes. Samples were incubated for 3 minutes at 56°C in a deparaffinization solution (Qiagen, 19093). A step of digestion with Proteinase K was performed followed by a treatment with DNAses. RNA was purified using the RNeasy MinElute column and eluted in 30uL of RNAse free water. Data were grouped into three categories based on experimental conditions: Vehicle (Sham) with 4 samples, MI with 4 samples, and MI+UA with 5 samples. RNA-seq library preparation and Illumina pair-end read sequencing RNA-seq library preparation and Illumina pair-end read sequencing Library preparation: Strand-specific cDNA library • rRNA depletion • mRNA fragmentation • Random primed cDNA synthesis (strand-specific) • Adapter ligation and adapter specific PCR amplification