GSM8994217: G378W, mutant, replicate 2; Drosophila melanogaster; RNA-Seq
Total RNA was extracted using the Quick-RNA Tissue/Insect Microprep Kit (Zymo Research, Cat # R2030) following the manufacturer's instructions Poly-A mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, first-strand cDNA synthesis was performed using random hexamer primers, followed by second-strand synthesis. End repair, A-tailing, adaptor ligation, size selection, amplification, and purification steps were conducted to prepare libraries.