GSM9679759: Inflammatory cytokine-treated only, replicate 3; Homo sapiens; ATAC-seq
After 24 hours of treatment, media was removed and cells were rinsed with PBS, then collected by trypsinization using TrypLE. Trypsin was neutralized with RPMI-1640 containing 10% FBS, and cells were centrifuged at 300 x g for 5 minutes. The supernatant was removed, cells were resuspended in PBS to remove residual media, centrifuged again at 300 x g for 5 minutes, and resuspended in fresh PBS for counting. A total of 50,000 cells per condition were transferred to 1.5 mL tubes. ATAC-seq libraries were prepared using the Zymo-Seq ATAC Library Kit (Zymo Research) according to the manufacturer's instructions. Libraries were purified using AMPure XP double-sided purification (Beckman Coulter) and assessed by Qubit fluorometry (Thermo Fisher Scientific), capillary electrophoresis, and quantitative PCR.