GSM8758202: Cortex_YB1_5; Mus musculus; RNA-Seq
RNA was extracted using RNAzol RT (Molecular Research Center, Cincinnati, OH, USA) according to the manufacturer's protocol. The concentration and quality of RNA were determined via a Qubit Fluorometer (Invitrogen) and an Agilent Bioanalyzer 2100, respectively, using an RNA 6000 Nano Kit (Agilent Technologies, Santa-Clara, CA, USA). RNA with an Integrity Number (RIN) no less than 8 was taken. An Illumina NEB Next Ultra II Directional RNA Library Prep Kit (NEB, Ipswich, MA, USA) was used for the mRNA library preparation