RNA-Seq of Korla fragrant pears: leaves
The samples were subsequently transported to Biomarker (Beijing, China) on dry ice for sequencing. Their total RNA was extracted using advanced molecular biology equipment. Following RNA extraction, the purity, concentration, and integrity of the RNA were assessed using a NanoDrop 2000 and an Agilent 2100. Subsequently, a library was generated, and a Qubit 3.0 fluorescence quantification instrument was utilized for preliminary quantification, with a target concentration of 1 ng/L or greater. The Qsep400 high-throughput analysis system was then employed to check the inserted fragments of the library. Finally, the librarys effective concentration (library effective concentration > 2 nM) was precisely quantified using the Q-PCR method to ensure the librarys quality. After the library quality inspection, PE150 sequencing was performed using the Illumina NovaSeq 6000 (Illumina, USA) sequencing platform.