Shotgun metagenomic sequencing of grapevine rhizosphere
For rhizosphere extractions, roots were washed in sterile phosphate buffered saline with 0.001% Silwet-77, vortexed, and shaken before the slurry was centrifuged to collect the rhizosphere pellet, which was then frozen at 80 C. DNA was extracted using the DNeasy PowerSoil Pro Kit with a 350 mg starting material followed by cleanup with the DNeasy PowerClean Pro Kit. Extracts were quantified on a DS-11 spectrophotometer. Shotgun metagenomic libraries were prepared by Novogene and sequenced on an Illumina NovaSeq 6000 with 2x150 bp reads.