RNA-seq of Varroa destructor foundress mites from brood cell of emerging adult honey bees
Seven foundress mites were homogenized using a Precellys Evolution homogenizer in tubes containing ten 0.5 mm stainless steel beads, two 2.3 mm stainless steel beads and 600 L of TRIzol reagent. Direct-zol MicroPrep (Zymo Research) kit was used to extract RNA from Varroa homogenate using manufacturers instructions with DNase treatment and an additional column wash step with 350 L of RNA Wash buffer prior to the final RNA Wash before elution in water.