PacBio Revio HiFi whole-genome sequencing of Stentor coeruleus
HMW genomic DNA (PacBio NanoBind PanDNA kit); short fragments removed by PacBio Short Read Elimination; sheared on a Megaruptor 3; SMRTbell library prepared with the SMRTbell prep kit 3.0 (whole-genome protocol); sequenced on a PacBio Revio (SPRQ chemistry, 30-hour movie), yielding HiFi (CCS) reads. Bauer Core Facility, Harvard University.