GSM9682719: HEK-293, pH 6.6, DMSO, repeat 1; Homo sapiens; RNA-Seq
Total RNA was isolated using RNeasy Mini Kit (Qiagen, USA) and treated with RQ1 Rnase-Free DNAse I (Promega, USA) to remove residual gDNA. Libraries were prepared using KAPA mRNA Capture Kit (Roche, Switzerland) and KAPA RNA HyperPrep Kit (Roche, Switzerland). Libraries were purified using KAPA HyperPure Beads (Roche, Switzerland), and size distribution and quality were assessed using a High Sensitivity DNA chip (Agilent Technologies, USA). Libraries were quantified using the Quant-iT DNA Assay Kit, High Sensitivity (Thermo Fisher Scientific, USA). Sequencing was performed on the NextSeq 1000 (Illumina, USA) using the NextSeq 1000/2000 P2 Reagents (200 Cycles), with 2% PhiX (Illumina) added as an internal control.