GSM9682426: C3H10T1/2 DNMT3A/B-double knockout (DKO) cells clone 1; Mus musculus; RNA-Seq
Total RNA was extracted from approximately 1 million cells using AllPrep DNA/RNA/miRNA Universal Kit (QIAGEN) according to the manufacturer's instructions, including on-column DNase I treatment. RNA quality was assessed using an Agilent 2100 Bioanalyzer prior to library preparation. RNA libraries for RNA-seq were generated using the TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat (Illumina) according to the manufacturer's instructions.