GSM9682161: Rat_hp_1 hippocampus RRBS, High intrinsic aerobic capacity, female, aged 23-24 months; Rattus norvegicus; Bisulfite-Seq
Hippocampal tissue was snap-frozen and stored at -80C. Total genomic DNA was extracted using the PureLink Genomic DNA Mini Kit (Thermo Fisher Scientific, USA) according to the manufacturer's instructions. RRBS libraries were prepared using the Premium RRBS Kit V2 (Diagenode, Seraing, Belgium). Briefly, 100 ng genomic DNA was digested with MspI, followed by end repair, adapter ligation, size selection using Agencourt AMPure XP beads, bisulfite conversion, purification, and PCR amplification. Final library quality was assessed by Qubit dsDNA HS Assay and Agilent Bioanalyzer. Sequencing was performed on an Illumina HiSeq 2500 in paired-end mode.