GSM9350178: 25928X3 25928_RAW264.7_MSNP_6h_3; Mus musculus; RNA-Seq
The total RNA of the cells was collected in RNase-free water using the Qiagen RNeasy Miniprep Kit. The sample was treated with DNase to remove DNA and stored at -80°C. RNA concentration was measured with a Qubit RNA HS Assay Kit (Fisher Scientific #Q32855).  RNA quality was evaluated with an Agilent Technologies RNA ScreenTape Assay (5067-5579 and 5067-5580). All samples were ensured to have an RNA quantity of over 25 ng/µL and an RNA integrity number (RIN) between 9 and 10 before sequencing. The library was generated using the NEB Next Ultra II Directional RNA Library Prep with Poly(A) mRNA Isolation kit for Illumina sequencing