GSM8458820: Inf_Air_B; Mus musculus; RNA-Seq
RNA was extracted in TRIzol (ThermoFisher) and precipitated from the aqueous phase using 1.5 volumes of 100% ethanol. The mixture was transferred to an RNA isolation column (Omega Bio-Tek), and the remaining steps were followed according to the manufacturer's recommendations. Reverse transcription followed by qPCR was performed in the same reaction using the Universal Probes One-Step PCR kit (Bio-Rad Laboratories) and the TaqMan primers: Ppib (Mm00478295_m1), Aqp5 (Mm00437578_m1), and Gkn3 (Mm01183934_m1) (all from Thermofisher). Libraries were prepared using a NEB ultraII directional kit (New England Biolabs), and 40 million paired-end reads were collected per library using an Illumina HiSeq (Illumina)