DNA panel sequencing and bulk RNA sequencing of established sarcoma 3D models in the MASTER precision oncology registry
RNA-seq libraries were prepared from 500 ng of high-quality RNA (RIN=9.1) according to the Illumina stranded total RNA Prep, Ligation with Ribo-Zero Plus (Illumina, San Diego, CA, USA) protocol and sequenced (275 bp paired-end) on an Illumina NextSeq500/550 platform.