Sedments from marine section of a stream within the Carpinteria Salt Marsh Reserve
Sediment DNA was extracted using the Power Soil DNA Isolation Kit  according to manufacturer (Qiagen, Germantown, MD, USA) directions. The V4-V5 region of the 16S rRNA gene was amplified using archaeal/bacterial primers with Illumina (San Diego, CA, USA) adapters on 5 end (515F 5-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-GTGYCAGCMGCCGCGGTAA-3 and 926R 5-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-CCGYCAATTYMTTTRAGTTT-3). Each product was barcoded with Illumina NexteraXT index 2 Primers that include unique 8-bp barcodes (P5 5-AATGATACGGCGACCACCGAGATCTACAC-XXXXXXXX-TCGTCGGCAGCGTC-3 and P7 5-CAAGCAGAAGACGGCATACGAGAT-XXXXXXXX-GTCTCGTGGGCTCGG-3). Products were purified using Millipore-Sigma (St. Louis, MO, USA) MultiScreen Plate MSNU03010 with vacuum manifold and quantified using ThermoFisherScientific (Waltham, MA, USA) QuantIT PicoGreen dsDNA Assay Kit P11496 on the BioRad CFX96 Touch Real-Time PCR Detection System. Barcoded samples were combined in equimolar amounts into single tube and purified with Qiagen PCR Purification Kit 28104 before submission to Laragen (Culver City, CA, USA) for 2 x 250bp paired end analysis on Illuminas MiSeq platform with PhiX addition of 15-20%.