GSM9429186: mandibular condylar cartilage, young mice(2 months)-6; Mus musculus; RNA-Seq
Total RNA was extracted from tissue samples using TRIzol reagent. Samples were homogenized, extracted with chloroform/isoamyl alcohol (24:1), precipitated with isopropanol at -20°C, washed with 75% ethanol, and dissolved in DEPC-treated water. mRNA was enriched using oligo(dT) magnetic beads and fragmented. Double-stranded cDNA was synthesized, followed by end repair, 3' adenylation, and adapter ligation. PCR amplification was performed, and the products were circularized to generate single-stranded circular DNA. DNA nanoballs (DNBs) were generated by rolling circle replication and sequenced on DNBSEQ platform using cPAS technology (PE150, strand-specific).