Genome Assembly of Pleurotus ostreatus PC80: Single-spore isolate
"Genomic DNA was extracted from a single-spore isolate (PC80) of Pleurotus ostreatus strain Hei Kang 650 using the CTAB method. High-quality sequencing libraries were constructed using a combination of PacBio HiFi, Illumina, and Hi-C technologies. A PacBio SMRTbell library (~15 kb) was prepared following PacBio's standard protocol and sequenced on the PacBio Sequel II platform, generating 8.73 Gb of HiFi reads with an N50 of 19,033 bp. Illumina short-read libraries (270 bp insert size) were prepared for error correction and sequenced using the PE150 strategy, producing 7.2 Gb of clean data. Hi-C libraries were prepared to capture chromatin interactions and sequenced on the Illumina HiSeq X Ten platform. The combined data enabled a chromosome-level genome assembly. Additionally, RNA-Seq libraries were constructed using the TruSeq Stranded mRNA Library Prep Kit and sequenced to generate transcriptional data for genome annotation."