GSM8995236: ThA1R;fly whole body;Thor[2];Lsp2[A10];RNA-Seq;rep1; Drosophila melanogaster; RNA-Seq
Whole female flies were ground in liquid nitrogen. A portion of the powdered tissue was reserved for total RNA extraction, while the remaining material was lysed and subjected to 10–45% sucrose density gradient centrifugation. Monosome and polysome fractions were collected after gradient fractionation. Total RNA and RNA from the monosome and polysome fractions were separately isolated using TRIzol reagent (Invitrogen, #15596026). Poly(A)+ RNA was enriched from 2 μg of total RNA, monosome-bound RNA, or polysome-bound RNA using VAHTS mRNA Capture Beads (Vazyme, #N401). The enriched RNA was fragmented and subjected to end repair, adaptor ligation, and PCR amplification using the VAHTS Universal V6 RNA-seq Library Prep Kit (Vazyme, #NR604). Following library quality control and quantification, the libraries were sequenced on an Illumina NovaSeq 6000 platform to generate 150-bp paired-end reads.