Atlantic salmon 16S rRNA - Ch-PC887
Paired-end 16S rRNA amplicon sequencing of the V1-V2 hypervariable region (primers 27F/338R) from Atlantic salmon gut tissue (PC) and water samples (ENV). DNA extracted using QIAamp DNA Stool Mini Kit. Sequencing performed on Illumina NovaSeq 6000 (2x250 bp). Raw reads processed using Cutadapt (v5.2) for primer removal and DADA2 (v1.34) for quality filtering, denoising and chimera removal. Taxonomy assigned using SILVA 138.2.