GSM8458831: Patient #1 Lesional biopsy; Homo sapiens; RNA-Seq
Punch biopsies  were incubated in 5 U/mL dispase at 37℃ for 45 min. The epidermis was peeled from the dermis, cut into fine pieces, and incubated in 0.25% trypsin-EDTA with 50 μg/mL DNase I for 45 min at 37°C, quenched with fetal bovine serum, and passed through a 70 μM cell strainer. Dermis was minced and digested in 0.1% collagenase II and 0.1% collagenase V in RPMI 1640 with 50 μg/mL DNase I for 90 min at 37°C, and strained through a 70-μM  cell strainer. Dead cell removal was performed using a Dead Cell Removal Kit. Epidermal and dermal cells were then recombined and washed in PBS containing 0.04% BSA and resuspended at a concentration of approximately 1,000 cells/µL. Library generation was performed following the 10x Genomics Chromium Single Cell 3ʹ v3.1 Reagents Kit (following the CG000315 Rev E. user guide), where we targeted 10,000 cells per sample for capture.