GSM9010239: Microglia, Trichinella spiralis day 10, F, biol rep 4; Mus musculus; RNA-Seq
Microglia were isolated from the brain of naïve and Trichinella-infected mice, subjected to flow cytometry-based sorting (live, TCRb-, B220-, P2RY12+, CD11b+, CD45.2 mid) and RNA extracted with TRIzol (Invitrogen) as per manufacturers instructions. Samples were analyzed on a Agilent 2100 Bioanalyzer, and only samples with an RNA integrity score (RIN) >8 were processed for library preparation following the standard protocol for Illumina Stranded mRNA prep (Illumina).