GSM9138327: GEX NBSGW, sample 2; Homo sapiens; RNA-Seq
Mice were euthanized 20 weeks post-transplant. Two femurs were collected and flushed with MACS buffer. Red blood cells were lysed using the lysing buffer (BD Biosciences, Franklin Lakes, NJ). The human cells from mouse bone marrow were enriched using human (hu)CD45+ microbeads (Miltenyi Biotec, Gaithersburg, MD). The huCD45+ cells were further purified by FACS sorting: murine CD45-, huCD19-, huCD3-, huCD33- and huCD34+ cells were sorted on Aria H Cell Sorter (BD) and sc-RNA libraries were prepped sc-RNA libraries were prepped using Chromium Single Cell 3' Gene Expression reagent kit (10x Genomics, Pleasanton, CA) according to protocol, starting with 10,000 CD34+ cells for NBSGW mice 1,000 CD34+ for NSG mice. Prepped libraries were sequenced on Novaseq XPlus.