GSM8453916: Human_FT_BRCA1m_C-21-71; Homo sapiens; RNA-Seq
Qiagen Allprep DNA/RNA FFPE kit following the manufacturer's protocol for recovering total RNA, including small RNAs 500 ng of total RNA using the KAPA RNA HyperPrep Kit (Kapa Biosystems, Wilmington, MA USA). Ribosomal RNA material was reduced using the QIAseq FastSelect –rRNA HMR Kit (Qiagen, Germantown, MD, USA). RNA was sheared to 300-400 bp and was converted to cDNA. cDNA fragments were ligated to IDT for Illumina TruSeq UD Indexed adapters (Illumina Inc, San Diego CA, USA)). Following adapter ligation, 8 cycles of PCR were performed.