GSM9678630: Mouse 10, Tumor 2, high dose DAC; Mus musculus; OTHER
Total RNA was purified from whole tumors using TriReagent and EconoSpin Columns. Tumors were homogenized by adding 2.8 mm Zirconium beads to the tissue, and homogenization was carried out using a Precellus Homogenizer run at 3 x 15 seconds at 6500 rpm. TCRa and TCRb receptor libraries covering the complemen-tarity determining region 3 (CDR3) were prepared using SMART-Seq Mouse TCR (with UMIs) (Takara, 634815) and SMARTer RNA Unique Dual Index Kit (Takara, 634451) following manufacturer's instructions.