GSM8828022: WT_4; Mus musculus; RNA-Seq
Cortices of kidneys from 3 - 4 mice from each of the cohorts were dissected for mRNA-seq. Five -10 mg of each cortex section was transferred into a tube containing beads (Benchmark Scientific Prefilled Tubes for Homogenizer, Catalogue #, D1032-15) and a 500 μl mixture of RLT (from Qiagen RNeasy Plus Mini Kit (Cat. No. 74134)) and β-mercaptoethanol. Tissues were homogenized twice for 30 seconds each with a Bead Bug homogenizer (Benchmark Scientific, Fort Lauderdale, FL). After homogenization, total RNA was extracted by following the protocol from the kit. Total RNA from RCC4 cells was extracted by following the protocol from the kit. RNA-seq libraries were prepared using the TruSeq Stranded mRNA Library Prep Kit (Illumina), following manufacturer's protocols.