GSM8993742: WT_exe#1; Mus musculus; RNA-Seq
For population-level RNA-seq, samples were double-sorted, and 1,000 MmSCs were collected into 5 microliters of TCL buffer (Qiagen) containing 1% 2-mercaptoethanol (Sigma-Aldrich) in DNA LoBind tubes (Eppendorf). For whole-tissue RNA-seq, samples were dissected and immediately frozen in liquid nitrogen until all samples were collected. Fresh-frozen tissues were homogenized in TRIzol followed by RNA isolation according to the manufacturer's protocol. 2 ng RNA were resuspended in 5 microliters of TCL buffer (Qiagen) containing 1% 2-mercaptoethanol (Sigma-Aldrich). Library construction and sequencing and data processing were performed according to the standard Immunological Genome Project protocols (https://www.immgen.org/img/Protocols/ImmGenULI_RNAseq_methods.pdf).