GSM9312200: Reaction2_HT; Mus musculus; OTHER
FACS-sorted, life human CD45+ cells isolated from MDA-MB-231 tumor cell suspensions were filtered through a 40 μm cell strainer and the concentration adjusted to 1000 cells/μL before single cell capture using the Chromium controller (10x Genomics). Cells were loaded on the Chromium Controller (10x Genomics) and Single-cell RNA-seq libraries were prepared using the Chromium Single Cell 3ʹ v3.1 Reagent Kit (10x Genomics) according to manufacturer's protocol.