GSM8759413: emf2 H3K4me3 (ChIP) rep2; Arabidopsis; ChIP-Seq
ChIP-seq was performed according to the protocol described by Kaufmann et al., 2010 (Kaufmann et al., 2010a). Sequencing was done at the Technion Genome Centre (TGC) according to Illumina's protocols. The TruSeq Nano DNA Library Prep Kit (FC-121) was used for library preparation. ChIP-enriched DNA fragments were size-selected on an agarose gel to isolate fragments approximately 200 bp in size. Indexed adapters, diluted 1:10, were added, and the library was amplified using PCR with 14 cycles (instead of the standard 8 cycles). Groups of 7–10 indexed libraries were loaded onto individual lanes of the Illumina HiSeq 2500 system. All samples were processed using the standard 50 bp single-end sequencing protocol.