GSM8892660: Mut_Yolk sac replicate2; Mus musculus; RNA-Seq
Yolk sac from E8.5 mouse embryos were dissected, disassocited into single cells, sorted for live cells before pool loading to 10x Chips. Cells were captured in droplets. Following reverse transcription and cell barcoding in droplets, emulsions were broken and cDNA purified using Dynabeads MyOne SILANE followed by PCR amplification per manual instruction. 10x library (3' scRNA-seq)