RNA-seq of Abies alba: leaves (pool)
Abies alba 3-year-old trees were subjected to eight different treatments (control, soft cold, extreme cold, heat, drought, darkness, growth hormones, physical damage) in control growth chambers (CGC). Total RNA was extracted from 100 mg of each sample using SpectrumTM Plant Total RNA Kit (Sigma-Aldrich, USA). Samples were then pooled by treatment (at least 1000 ng of RNA in each pool, adding the same amount of each sample): 24 different pools (8 treatments with 3 pooled samples for each of the 3 tissues). mRNA libraries were constructed and sequenced at Macrogen (Korea): TruSeq Stranded mRNA Library Prep Kit was used to construct the short-read libraries which were sequenced on Illumina NovaSeq X (9 Gb/sample).