GSM8860560: HCC364 Hi-C Rep2; Homo sapiens; Hi-C
Hi-C libraries were prepared using the Arima Genome-Wide Hi-C+ kit. DNA from two pellets of each cell line were titred for DNA content and prepared according to the Arima protocol. DNA was sheared in an 8 tube microtube strip (Covaris) in Covaris Shearing Buffer (10 mM Tris-HCl pH 8, 1 mM EDTA, 0.1% SDS) on a Covaris LE220sc at PIP 450/DF 15/CBP 200 for 90 seconds total. Hi-C libraries were prepared using the Arima Genome-Wide Hi-C+ kit and sequenced as 2x60bp on a NovaSeq SP.