GSM8509647: p1mCIP_2; Rhizopus microsporus; ChIP-Seq
R. microsporus mycelium was crosslinked with formaldehyde at a final concentration of 1% for 20 minutes in a shaker (80rpm). Crosslinking was quenched by adding glycine at a final concentration of 135 mM for 10 minutes. The lysates were sonicated using a Bioruptor Pico sonicator (Diogenode) with 30s ON/OFF pulses for 25 cycles in a thermostated bath (4 ºC). Truseq library preration kit was used and 30 ng were used for library preparation