16S rRNA gene sequencing of gut microbiota: male mus musculus
C57BL/6J mice were divided into 4 groups: (1) N group: mice were fed with normal diet; (2) HFD group: mice were fed with high-fat diet; (3) O group: mice were fed with high-fat diet supplemented with 20% okara; (4) MO group: mice were fed with HFD supplemented with 20% modified okara. After 8-week intervention, fecal samples were collected and analyzed for microbiome composition using 16S rRNA gene sequencing.