GSM9570158: *AB matched WT sibling zebrafish-M.marinum granuloma cells; Danio rerio; RNA-Seq
Granulomas were transferred to a 1.5 mL tube and cells were dissociated using 1 mL of 0.05% trypsin/EDTA (ThermoFisher Scientific, 25300054) at 30_x000E_C for 35 minutes with rocking. Cells were collected by centrifugation for 5 min at 300 xg, resuspended in 1x DPBS with 10% FBS on ice, and passed through a 40 mm strainer. Single cell samples were submitted to the Molecular Genomics Core at Duke Molecular Physiology Institute for library preparation, using Chromium Single Cell 3' GEM, Library and Gel Bead Kit v4, and gem generation using the 10x Genomics platform. Libraries were sequenced was at Duke Sequencing and Genomic Technologies core facility.