RNA-Seq of Pterinochilus murinus: adult female venom glands
The RNA library was prepared using theNEBNext Poly(A) mRNA Magnetic Isolation Module and the NEBNext Ultra II DirectionalRNA Library Prep Kit (New England Biolabs), following the manufacturers protocol. The finallibrary was cleaned up using the Agencourt AMPure XP system (Beckman Coulter). Thelibraries size distribution and quality were assessed using a high-sensitivity DNA chip (AgilentTechnologies), and they were subsequently quantified with the Quant-iT DNA Assay Kit, HighSensitivity (Thermo Fisher). Equimolar quantities of all libraries (10 pM) were sequenced in ahigh-throughput run on the Illumina HiSeq 2500 platform, using 2  100 bp paired-end reads anda 1% Phix spike-in control.