GSM8966838: HEL tumor, Vehicle Control 3; Homo sapiens; RNA-Seq
For RNA-seq analysis, four tumors per group from mice bearing HEL flank tumors treated with vehicle control or BAY 2666605 were processed into a single-cell suspension for subsequent isolation of RNA with RNEasy Plus Mini Kit as described by the manufacturer (Qiagen). RNA was reconstituted in RNase-free water. RNA quality and quantity were assessed by Bioanalyzer RNA pico chip and Qubit RNA HS assay kit, respectively. One thousand nanogram of RNA was used as input for library construction using Illumina stranded total RNA prep with Ribo-zero plus (Illumina 20040529) according to manufacturers' protocol with the following modification: To retain the ribosomal RNA, we skipped the ribosomal RNA depletion step and started with RNA fragmentation and denaturation directly.