AGO2-IP miR-seq: HM3
Small RNA libraries for miR-seq were generated using the QIAseq miRNA Library Kit (Qiagen, 331502) according to the manufacturer's instructions. RNA from AGO2-IP from a 10cm dish was used for library preparation. The library was size-selected and purified on a native 6% (w/v) acrylamide gel and ethanol precipitation, respectively. The library was sequenced on the Illumina Nextseq 1000 platform.