GSM8859559: Control group replicate3; Mus musculus; miRNA-Seq
Total RNA was extracted from tissue using the TRIzol reagent following the manufacturer's protocol. RNA concentration was quantitated using the NanoDrop ND-100 Spectrophotometer All RNAs were extracted from injured mice WAT of the CIH models. The extracted RNA samples were subjected to agarose gel electrophoresis and NanoDrop quality inspection and quantification, after which RNA-seq library was constructed. The mixed sequencing libraries of different samples were denatured with 0.1M NaOH to generate single-stranded DNA, which was then sequenced on an Illumina sequencer following the manufacturer's instructions.