LMIC human fecal stool sample CAM011209-056645, whole-genome shotgun sequencing
DNA sequencing libraries were prepared using Illumina DNA Prep Tagmentation kit (Illumina, San Diego, CA), following the manufacturers instructions except for the following changes. The insert size was increased to a range of ~375-1100 bp by reducing the 1st and 2nd volumes of Sample Purification Beads to 40 l and 11 l, respectively. This modification resulted in larger inserts compared to the mostly below 300 bp inserts obtained using the manufacturers protocol. Libraries were quantified in 10 l volume reactions and 90-s annealing/extension PCR, and then pooled and normalized to 4nM. Pooled libraries were re-quantified by ddPCR on a QX200 system (Bio-Rad, Hercules, CA), using the Illumina TruSeq ddPCR Library Quantification Kit and following the manufacturers protocols. Libraries were sequenced using a MiSeq Reagent Kit v2 (500-cycles) on a MiSeq instrument (Illumina) at 16 pM, following the manufacturers protocols.