GSM8603721: Liver_Cirrhotic :CH_09; Homo sapiens; RNA-Seq
Tissues were snap-frozen and minced with a mortar and pestle under liquid nitrogen. The resulting tissue powder was dissolved in Trizol (Invitrogen 15596-018). RNA was separated from DNA and proteins with a Trizol-Chloroform centrifugation and was subsequently precipitated with isopropanol. The RNA pellet was then resuspended in nuclease-free water. Sequencing libraries were prepared following the protocol included with the kit TruSeq Stranded mRNA Sample Preparation Guide (Part #15031047 Rev. E)