GSM8603614: AD-bMSC, Knockout-Library, day16, Replicate 1, Amplicon-seq; Bos taurus; OTHER
Total DNA was extracted from 5x10^5 cells using QuickExtract reagent (Lucigen) followed by two consecutive PCR to amplify sgRNA amplicon Extracted genomic DNA was amplified and added unique sample adapters, followed by SPRI-beads size selection and a second PCR adding NGS adapters, followed by a second SPRI-beads size selection