GSM9312675: Peripheral CD8+ T cells, siControl transfected, anti-CD3/CD28 activated, 24-hours, healthy donor 3 (ATAC-16); Homo sapiens; ATAC-seq
100,00 nucleofected CD8+ T cells were prepped following the published OMNI ATAC-seq protocol. Amplified libraries were purified and the size of library DNA was determined with a fragment analyzer (Advanced Analytical Technologies) using the High Sensitivity NGS Fragment Analysis Kit (Advanced Analytical Technologies). Enrichment of accessible regions was determined by the fold difference between positive and negative genomic loci using real-time PCR. The libraries were sequenced to 51 base pairs from both ends.