GSM8758998: P20 mouse cortex [hypoxia]; Mus musculus; RNA-Seq
P20 mouse cortical tissues (3 animals combined per group) were treated with papain and DNase I at 37 degree for 30 min. Unhealthy cells were removed using debris and dead cell removal kit with MS separation columns. The cell suspension was prepared using single-cell 3' Library and Gel Bead Kit V3.1 and the Chromium Single Cell G Chip Kit, and then loaded onto the Chromium Single Cell Controller (10x Genomics) to generate single-cell beads in the emulsion according to the manufacturer's protocol.