GSM8993977: Organoid, IFN, 6hr, rep1; Mus musculus; RNA-Seq
RNA was isolated using the GeneJET RNA purification kit (Thermo). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library. For the non-directional library, it was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.