GSM9139911: CTCF-C8_DMSO_HiC; Homo sapiens; Hi-C
Cells were fixed and processed according to the Arima Genomics HiC+ Kit protocol. Proximity-ligated DNA was first sonicated to ~400 bp using a Covaris M220 with 50 W power, 10% duty factor and 200 cycles per burst for 78 seconds, and then size-selected using AMPure beads. Lbrary preparation was performed using KAPA HyperPrep (Roche #KK8502) according to instructions provided with the Arima Genomics HiC+ Kit, with library preparation done on streptavidin beads.