Organelle genome assemblies of Nitzschia nienhuisii
The cell pellet from strain 14VII23-3B NitzneinNCP067 was extracted using the QIAGEN DNeasy Plant Minikit (QIAGEN Sciences, Valencia, CA, USA) following the manufacturers protocol, with all incubation times doubled and the addition of a 45 second Mini-BeadBeater-16 (Biospec Products, Bartlesville, OK, USA) cycle using 1.0 mm glass beads during the initial cell disruption step. Following extraction, the DNA was then amplified using the QIAGEN Repli-g Single Cell Kit (QIAGEN Sciences, Valencia, CA, USA), following the manufacturers protocol for amplification o purified genomic DNA. Post-amplification, the DNA concentration of the extraction was measured using the double-stranded DNA Broad Range kit (Life Technologies Corporation, Oregon, USA) in an Invitrogen Qubit v3 fluorometer (ThermoFisher Scientific, Germany). DNA was then submitted for high-throughput sequencing using the Illumina NovaSeq platform and chemistry (Illumina, Inc, San Diego, CA, USA).