GSM8966439: Calvaria-WT-4; Mus musculus; RNA-Seq
Calvaria were isolated from 2- to 3-day-old female WT (n=4) and Girk3-/- (n=4) mice. The calvaria were isolated, washed with PBS, then snap frozen. Calvaria were homogenized in 1 mL Trizol, then total RNA was extracted using the Zymo Direct-zol RNA Miniprep Kit. RNA Sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina per the manufacturer's instructions (NEB, Ipswich, MA).