scRNA-seq of homo sapiens: AGA male occipital HFs
The sequencing library was constructed using the DNBelab C series high-throughput single-cell RNA library preparation kit (BGI Genomics). Single-cell suspensions were loaded onto the DNBelab C4 system to form droplet-based libraries. Reverse transcription and second-strand synthesis were performed to generate cDNA, which was then amplified and purified. Specific scRNA barcode primers were added for library amplification. Library concentration and fragment size were assessed using Qubit and Agilent Bioanalyzer.