U4_H (CRX2052073; CRA031056)
DNA extract was fragmented to an average size of about 350 bp using Covaris M220 (Gene Company Limited, China) for paired-end library construction. Paired-end library was constructed using NEXTFLEX  Rapid DNA-Seq (Bioo Scientific, Austin, TX, USA). Paired-end sequencing was performed on Illumina NovaSeq X Plus (Illumina Inc., San Diego, CA, USA) at Majorbio Bio-Pharm Technology Co., Ltd. (Shanghai, China) using NovaSeq X Series 25B Reagent Kit according to the manufacturer's instructions (www.illumina.com).