GSM9210822: Males-5xFAD_2; Mus musculus; RNA-Seq
RNA was isolated using the RNeasy Plus Micro Kit (Qiagen, Valencia, CA). The concentration of RNA was determined using the NanoDrop One spectrophotometer (NanoDrop, Wilmington, DE), and RNA quality was assessed with the Agilent Bioanalyzer 2100 (Agilent, Santa Clara, CA). Pre-amplification of 1 ng of RNA was performed with the SMART-seq v4 Ultra-Low mRNA Input kit (Clontech, Mountain View, CA) per manufacturer's instructions. The quantity and quality of the cDNA were determined using Qubit Fluorometer (Life Technologies, Carlsbad, CA) and the Agilent Bioanalyzer 2100 (Agilent, Santa Clara, CA). A total of 150 pg of cDNA was used to generate Illumina-compatible sequencing libraries with the NexteraXT library preparation kit (Illumina, San Diego, CA) per manufacturer's instructions.