GSM8720633: Gaq primary lung fibroblasts, Unstimulated_control, rep 3; Mus musculus; RNA-Seq
RNA was isolated using the RNAqueous isolation kit (Invitrogen, cat# AM1931) by following the manufacturer's instructions for guanidinium-based lysis/denaturant and glass fiber filter separation technology. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. (Done by Novogene Co)