Nanopore long-read sequencing of non-axenic culture
Genomic DNA was extracted from a non-axenic culture of FBCC-A233 using the Mag-Bind Universal Pathogen Kit (Omega Bio-Tek). Quality and concentration were checked by NanoDrop 2000 and Qubit 4 (dsDNA HS Assay). Library was prepared with the SQK-LSK114 kit and sequenced on an Oxford Nanopore Flongle R10.4.1 flow cell (FLO-FLG114).