Illumina MiSeq paired end sequencing of SAMD01823841
Genomic DNA was extracted from PANC1 cells cultured under pH 6.8 at day 24 using the NucleoSpin Tissue Kit. sgRNA cassettes were amplified following the TKOv3 protocol (Addgene), using 2.5 ug of genomic DNA per 50 uL PCR reaction with KAPA HiFi Reaction Mix. Primers containing Illumina adapter sequences and sample-specific barcodes were used. PCR products were resolved on 1% agarose gels, gel-purified, and subjected to sequencing.