GSM8720574: H3K4me3_ChIP_ADE_d6H+; Mus musculus; ChIP-Seq
Native ChIP was performed on 0.5–1M flow sorted ADE cells and immunopurified with either anti-H3K4me3 (07–473; Millipore) or anti-H3K27me3 (07–449; Millipore) as previously described (PMID: 27723457). ChIP libraries were prepared according to (PMID: 22855832) with modifications described in (PMID: 27723457). Independent replicate sample libraries (n = 2/3) were combined into equimolar pools prior to sequencing. Library PCRs were supplemented with 2× SYBR dye (Sigma S9430) so that amplification could be monitored by quantitative PCR on a Roche LightCycler 480. ChIP/Input libraries were sequenced individually or as multi-sample pools distinguished using single end barcoding primers (NEBNext multiplex oligos for Illumina, set 1-4). Libraries were sequenced on an Illumina Hi-seq 4000 system following the standard Illumina protocol to generate single-end 42 or 49 bp reads (BGI or The Danish National High-Throughput DNA Sequencing Centre).