ITS fungal DNA seq: FROGC1_515
DNA was extracted from homogenized soil samples using the PowerSoil Pro DNA extraction kit. Samples were then processed with metabarcoding amplification and Illumina NovaSeq Sequencing through the University of Tennessee, Knoxville Sequencing Core facility. ITS2 fungal primers (5.8S-Fun - ITS4-Fun) were used for broad coverage of fungal reads.