GSM9682395: HS-SY-II ATAC-seq, WA-68 (100 nM), 24 h, biol rep 2; Homo sapiens; ATAC-seq
ATAC-seq was performed using the Omni-ATAC protocol. 50,000 cells were pelleted and resuspended in 50 ul cold ATAC-RSB (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 3 mM MgCl2) with 0.1% NP40, 0.1% Tween-20, and 0.01% digitonin, incubated on ice for 3 min. After washing, pellets were resuspended in 50 ul transposition mixture (25 ul 2x TD buffer, 2.5 ul transposase [Illumina #20034198], 16.5 ul PBS, 0.5 ul 1% digitonin, 0.5 ul 10% Tween-20, 5 ul H2O) and incubated at 37C for 30 min at 1000 rpm. Tagmented DNA was purified using a DNA Clean and Concentrator kit. Tagmented DNA was PCR-amplified with indexed Nextera primers and purified for Illumina sequencing.