RNA-Seq of H9-induced oral epithelial cell
RNA concentration and purity were quantified using a NanoDrop 2000 spectrophotometer. RNA integrity was assessed using the Agilent 2100/LabChip GX system, and the RNA was subsequently used to generate cDNA libraries. Library quality was evaluated using the Qsep400 high-throughput analysis system, after which PE150 mode sequencing was performed on a high-throughput sequencing platform. The raw sequencing data were filtered to obtain Clean Data, which were then aligned to the specified reference genome to generate Mapped Data. Library quality assessment, structural-level analysis, differential expression analysis, gene function annotation and functional enrichment analysis were all performed using the BMKCloud platform (www.biocloud.net).