LdoS J-IP Dynabeads
DNA fragments containing base J were immunoprecipitated using antibodies against base J (PMCPMC3684241) and Protein A Dynabeads. After purification using AMPure XP beads, they were end-repaired and ligated with adapters before PCR amplification using NEBNext Ultra II Q5 High Fidelity DNA Polymerase.