scRNA-Seq of pig kidney
Cell suspensions (2105cells/ml, 100l) were loaded into microfluidic devices (Matrix1.0.1) and the separation of single cells was completed according to the principle of Poisson distribution. scRNA and scTCR/BCR libraries were constructed following the protocol of sCircle Single Cell Full Length Immuno_TCR/BCR Library Kit (Biotechnologies). Specifically, poly(A) tails were captured by magnetic beads with molecular markers. Cells and mRNA were labeled after the cells were lysed. The magnetic beads in the chip were collected, and mRNAs were reverse-transcribed into complementary DNA (cDNA) and amplified. After local cDNAs were fragmented and spliced, transcriptome sequencing libraries suitable for the Illumina sequencing platform were constructed.The nuclei suspension was loaded into the Chromium Next GEM Chip H with 10x reagents and barcoded with a 10 Chromium Controller (10X Genomics). DNA fragments from the barcoded cells were subsequently amplified and sequencing libraries were constructed with reagents from a Chromium NextGEM Single CellATAC Reagent Kits v2 (10X Genomics) according to the manufacturers instructions. scATAC-seq libraries were prepared according to the ChromiumNextGEM Single Cell ATAC Reagent Kits v2 User Guide Rev A (10x Genomics CG000496).