RNA-Seq from Primate iPSC derived Astrocytes (iAstrocytes)
RNA-Seq from Primate iPSC derived Astrocytes (iAstrocytes)
Cells (iAstrocytes) were detached with Accutase, counted and 500,000 cells were lysed with TRI-Reagent (Sigma). iAstrocytes were grown on hESc-qualified Matrigel (Corning)-coated plates, in Astrocyte Medium (ScienCell). Seeding density was 15,000 cells/sq. cm. RNA isolation was isolated using Direct-Zol RNA Isolation kit (ZYMO), according to the manufacturer's instructions. 1 ug of RNA (RIN > 8) was used for library constructions. Libraries were prepared using KAPA HyperPrep Kit, according to manufacturer's instructions. UDI/UMI (itDNA) indexes were used. Library quality was assessed with 4200 TapeStation System, High Sensitivity D1000 ScreenTape (Agilent).