Metagenome Genomic Sequences of NC2 (CRX2069465; CRA031462)
Paired-end libraries were prepared using the VAHTSTM Universal Plus DNA Library Prep Kit (ND617-02) according to the manufacturer's instructions. Total genomic DNA was enzymatically fragmented using the FEA Enzyme Mix. The resulting fragments were end-repaired, ligated with Illumina adapters and purified. VAHTSTM DNA Clean Beads (N411-03) were used to enrich fragments with a central size distribution of 430-530 bp (average range: 420-580 bp). Library quality was verified using Qsep-400 (fragment size analysis) and Qubit 3.0 (concentration measurement). Libraries meeting the following criteria were accepted for sequencing: concentration >=1 ng/microliter, unimodal peak profile, absence of adapter dimers or secondary peaks