The original sequence of NGS of Staphylococcus aureus: environment
Genomic DNA was extracted using a standard bacterial genomic DNA extraction kit. The sequencing library was prepared using the Illumina TruSeq DNA Sample Preparation Kit (or equivalent), following the manufacturer's instructions. Briefly, genomic DNA was fragmented by sonication, end-repaired, A-tailed, and ligated with Illumina sequencing adapters. Fragments of appropriate size were selected and enriched by PCR amplification. The final library was quantified and sequenced on an Illumina NovaSeq 6000 platform, generating paired-end reads (2  150 bp).