GSM9643375: Experiment 120 (lung from WT and Clec1a deficient mice at day 3 after primary E Coli infection) scRNAseq; Mus musculus; RNA-Seq
Cells were incubated with TotalSeq barcoded-antibodies for each sample, in order to identify their origin after RNA library creation. Approximately 16,000 cells per reaction were processed using a 10X Chromium Next Controller following the 3' V3 protocol. RNA libraries were then prepared using the Chromium Single Cell 3′ Library and Gel Bead Kit v2 (PN-120267) and Chromium Next GEM Single Cell 3′ Library and Gel Bead Kit v3.1 (PN-1000121) (10X Genomics)