GSM8459589: Dnmt3a, replicate 3, RNA seq; Mus musculus; RNA-Seq
RNA was isolated using the Direct-zol RNA MicroPrep Kit (Zymo research) according to the manufacturer's protocols. RNA was quantified using the Quant-iT RiboGreen assay (Life Technologies) and quality-checked by 2100 Bioanalyzer RNA 6000 Nano assay (Agilent) or LabChip RNA PicoSensitivitiy assay (PerkinElmer) before library generation. Libraries were prepared from total RNA with TruSeq Stranded Total RNA Library Prep Kit according to the manufacturer's instructions (Illumina).