GSM8452960: Blood_D0; Mus musculus; RNA-Seq
C3H/HeN mice were infected intranasally with 2e7 PFU of VEEV TC-83 for  2, 4, or 6 days. Uninfected mice were dosed in parallel with PBS. Mice were euthanized by exsanguination via cardiac perfusion of saline and brains were removed. PBMCs were isolated from effluent blood. Brain tissue was enzymatically digested and immune cells were isolated using percoll gradient centrifugation. Immune (CD45+) cells from uninfected (D0) and infected (D2, D4, D6) brains or uninfected (D0) and infected (D6) blood  were sequenced using Chromium Single-cell 3ʹ GEM, Library & Gel Bead Kit v3 (10x Genomics Cat # 1000075) on a 10× Genomics Chromium Controller following manufacturers' protocol.