GSM9137961: SW1990, EPZ-2; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol Reagent (Invitrogen) according to the manufacturer's instructions. RNA quality and integrity were assessed using the Agilent 2100 Bioanalyzer, and RNA concentration was measured with a Qubit Fluorometer. mRNA was enriched from total RNA using oligo(dT) magnetic beads. After fragmentation, first-strand cDNA was synthesized using random hexamer primers, followed by second-strand cDNA synthesis using dUTP. The cDNA fragments then underwent end repair, A-tailing, adapter ligation, USER enzyme digestion, PCR amplification, and purification. Libraries were quantified using Qubit and real-time PCR and size-checked with a Bioanalyzer.