GSM9023435: G5: 100 µg MD39.3 protein at week 8 VDJ; Macaca mulatta; RNA-Seq
Env-binding memory B cells at weeks 8, 10, 24, 26 and 32 in the 100 µg membrane-bound MD39.3 mRNA and 100 µg MD39.3 protein plus SMNP groups were stained with tetramerized BG505 MD39.3 probes using fluorescent-SA and TotalSeq-C oligo-tagged fluorescent-SA, surface antibodies and six different TotalSeq-C oligo-tagged anti-b2M & CD298 antibodies to multiplex animal samples. Samples were sorted on a FACSymphony S6 (BD Biosciences) into 1.5 mL tubes with FBS supplemented RPMI media. Libraries were constructed according to the manufacturer's protocol using the Chromium Next GEM Single Cell 5' Reagent Kit v2.