Bulk shotgun metagenome sequencing of Homo sapeins : inflamed tissue of CD4
Genomic DNA quality and quantity were assessed via fluorometry (Qubit, Invitrogen) and gel electrophoresis. Samples with a DNA Integrity Number (DIN)  6, as measured by an Agilent 4200 TapeStation, were used for further analysis. Library preparation was performed using the Illumina TruSeq Nano DNA Library Prep Kit. For each sample, 100 ng of DNA was fragmented to 350 bp using a Qsonica 800 R2, followed by Illumina adapter ligation and PCR amplification. Libraries were size selected to 500-600 bp and quantified using TapeStation 4200 (Agilent Technologies) and KAPA Library Quantification Kit (Kapa Biosystems). Sequencing was performed on an Illumina NovaSeq 6000 platform using 150 bp paired-end reads.