GSM8655161: CRC tumor tissue P09; Homo sapiens; OTHER
Tumor tissues from CRC patients (OrgaRES consortium study, France) were cryopreserved by snap freeze in Tissue-TekR OCT-compound and 10µm cryosections were subjected to the 10x Visium gene expression analysis according to the manufacturer guidelines. The optimal tissue permeabilization time used was 30 min. A Hematoxylin-Eosin staining was done after the tissue section was deposited onto the Visium slide capture area, images were obtained by Axioscan (Zeiss) Visium spatial gene expression Slide and Reagent kit (at the Go@L-GFS platform (PBLS - UAR2014 - US41, Lille France)) according to 10x Genomics guidelines;  The libraries were realized and sequenced on the NovaSeq 6000 system (Illumina).