Cut&Tag of human iPSC-derived neurons
Cut&Tag was performed using Epicypher kit 2.0 with either anti-STAT1 or H3K4me3 with IgG control antibody from the kit. 100k nuclei were used per reaction. DNA input was normalized per sample before multiplexed sequencing. Sequencing was performed using the Nextseq550 mid-output kit with 150 cycles (Illumina). 4-16M reads were obtained per sample.