ChIP-seq of C. elegans N2:Sample1
The worms which fed on E. coli OP50 were collected after reaching to adult stage. The chromatin was sheared into 0.2- to 1-kb fragments. The soluble chromatin fraction was immunoprecipitated using antibodies specific for H4K5ac (Abcam, ab51997) or H4K8ac (Abcam, ab45166). ChIP-seq libraries were prepared using the NEBNext UltraDNA library prep kit (New England Biolabs) followed with paired-end sequencing on an Illumina NovaSeq6000.