18S rRNA gene sequneces of a fungal colony cultured from senesced Populus deltoides wislizeni leaves submerged in the middle Rio Grande
DNA was extracted using a modified CTAB method with phenol:chloroform:isoamyl alcohol purification; PCR was performed in triplicate to amplify the V9 region of the 16S rRNA gene using Illumina 1391f and EukBr primers; SequalPrep was used to clean up and normalize PCR products; a Nextera XT DNA Library Preparation Kit was used to index and prepare sequencing libraries; libraries were purified following a AMPure XP bead cleanup protocol; the dual-indexed, paired-end amplicons were sequenced on an Illumina MiSeq at the University of New Mexico