WGS of Acinetobacter phage vB_AbaA_SWMUZ8: novel lytic phage
The bacteriophage lysate was treated with 200 U of BenzoNuclease (Novoprotein Scientific Inc., Suzhou, China) and 0.1 mg/mL RNase A (Sangon Biotech, Shanghai, China) to degrade the host nucleic acids. The DNase was subsequently inactivated by heating at 65 C for 10 min. The phage genomic DNA was extracted with the Qiagen MinElute Virus Spin Kit (Hilden, Germany), according to the manufacturers protocol. The extracted DNA was quantified with the Equalbit 1  dsDNA HS Assay Kit (Vazyme Biotech Co., Ltd, Nanjing, China) on a Qubit 3.0 Fluorometer (Thermo Fisher Scientific Inc., Waltham, MA, USA), and the genome size was assessment with 0.8% agarose gel electrophoresis. A sequencing library was constructed with the VAHTS Universal Plus DNA Library Prep Kit (Vazyme Biotech Co., Ltd), and its quality was rigorously verified on the Agilent 4200 Bioanalyzer system. After passing quality control, whole-genome sequencing was performed on the DNBSEQ-T7 platform.