GSM8457687: P22 ccRCC, ischaemia, rep2; Homo sapiens; RNA-Seq
Tissue RNA was extracted (AllPrep DNA/RNA Mini Kit, Qiagen, Germany) and quantified using the 4150 Tapestation system (Agilent, cat.no. G2992AA). Library preparation was performed using SMARTseq v4 low-input mRNA (Takara Bioscience, cat.no. 634893). After obtaining cDNA, the Nextera XT workflow (Illumina, cat.no. FC-131-1096) was used to perform the adapter ligation using the tagmentation protocol. The libraries for each sample were then quantified and pooled together using both the Qubit 2.0 (Invitrogen) and qPCR system (Aria MX real time PCR system, Agilent). The KAPA library quantification kit (Roche) was used for qPCR