Whole genome sequencing of CRE isolate using Illumina NovaSeq X Plus platform
Whole genome shotgun sequencing of carbapenem-resistant Enterobacterales (CRE) isolate. Genomic DNA was extracted using the Universal Genomic DNA Extraction Kit (Magnetic Bead Method). DNA quality was assessed by 1% agarose gel electrophoresis, NanoDrop 2000 spectrophotometer, and Quantus Fluorometer. Qualified DNA was fragmented by ultrasonication, end-repaired, 3'-adenylated, and ligated with Illumina adapters. Fragments of ~350 bp were enriched by magnetic beads, followed by PCR amplification to construct the sequencing library. Library quality was verified by capillary electrophoresis, qPCR, and NanoDrop. Sequencing was performed on the Illumina NovaSeq X Plus platform using the PE150 strategy, generating 150-bp paired-end reads. Raw reads were processed with fastp v1.0.73 to remove low-quality reads, PCR duplicates, and adapters, resulting in clean paired-end reads.