GSM8510580: MO_rep2; Homo sapiens; RNA-Seq
Monocytes and osteoclast precursor cells were sorted from human PBMC. Two biological replicates pooled from eleven independent donors (n=5 and n=6) were used for RNA-sequencing. Total RNA was extracted using RNeasy mini kit (Qiagen, Hilden, Germany). True-seq RNA Library preparation kits (Illumina, Inc., San Diego, CA) were used to purify poly-A+ transcripts and generate libraries with multiplexed barcode adaptors following the manufacturer's instructions. All samples passed quality control analysis on a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA).