GSM8510450: CD103+CD56+ ILCs sorted from sample OV738; Homo sapiens; RNA-Seq
RNA was extracted from sorted patient-matched CD103+CD56+ ILCs and CD103-CD56+ ILCs (N=4) using RNeasy Mini Kit (catalog no. 74104, Qiagen) according to the manufacturer's instructions. Quantity and quality was assessed using Agilent Bioanalyzer RNA Nano 6000 Kit (catalog no. 5067-1511, Agilent) and KAPA ZYBRR FAST qPCR kit (catalog no. KK44602, Roche). RNA libraries were prepared and ribosomal RNA were removed using SMARTer Stranded Total RNA-seq Kit V3 – Pico Input Mammalian (catalog no. 634485, Takara Bio USA Inc.). Libraries were normalized, pooled, and loaded onto Illumina Novaseq V1.5 catridge for cluster generation and sequencing on an Illumina Novaseq 6000 instrument (Illumina).