smallRNA-seq of Homo sapiens : small extracellular vesicles and blood serum of colorectal cancer
Small RNA libraries were prepared using QIAseq miRNA Library Kit (Qiagen, Hilden, Germany) according to the manufacturers protocol with the modifications for low RNA inputs (5 l, < 10 ng). Unique molecular indices were used to eliminate PCR and sequencing bias. All libraries were diluted to 4 nM and pooled in equimolar ratio. Denatured and diluted PhiX Control v3 was added at 1% and RNA sequencing was performed using the NextSeq 500/550 High Output v2 Kit and the NextSeq 500 Sequencing System (Illumina, San Diego, CA, USA).