Dual RNA-seq of chickpea (Cicer arietinum) infected with Ascochyta rabiei: leaf tissue from mature chickpea sampled at 72 hours post inoculation with Ascochyta rabiei (isolate AR0231)
Total RNA was extracted from leaf and pod tissues of mature chickpea plant inoculated with Ascochyta rabiei. Transcriptome sequencing was performed using the Illumina Stranded Total RNA Prep, Ligation with Plant Ribo-Zero Plus kit. 100 ng of RNA was mixed with the Hybridize Probe and rRNA Depletion Master Mix for library preparation. Paired-end sequencing was conducted at the Australian Genome Research Facility (AGRF, Melbourne, VIC, Australia). Sequencing was performed on the NovaSeq 600 System using a 2x150 bp run configuration and libraries were run on the S4 flow cell. The reads were demultiplexed in fastQ files and provided here.