GSM8701924: RNATCRseq_Rechallenge_ 8dpi_GP33pos_Lane1_TCR; Mus musculus; OTHER
At 8 days post-rechallenge, cells were enriched for CD8+ T cells using magnetic separation and stained with sample-specific LMO, viability dye, GP33 tetramer,and surface antibodies. Live CD8+ Tetramer+/- donor T cells were sorted based on congenic allele expression using a BD FACSAria into RPMI supplemented with 10–50% FBS. Recipient GP33+ T cells were also sorted as controls. After sorting, samples were pooled and loaded into the Chromium Controller (10x Genomics). scRNA and TCR libraries were prepared according to manufacturer's instructions (10X Genomics 5' v2). MULTI-seq library preparation workflow performed following cDNA amplification, as described previously (McGinnis, Patterson, et al., Nature Methods, 2019).