16S rRNA gene sequencing of Lithobates clamitans: tadpole whole gut
Genomic DNA was extracted from tadpole gut, tadpole skin, and tank water samples using the QIAamp PowerFecal Pro DNA kit (Qiagen, Cat. No. 51804) following the manufacturers protocol with blanks as kit controls. The V4 region of the 16S rRNA gene was amplified using the 515F and 806R primers and sequenced on the Illumina MiSeq platform.