GSM8510429: GRB2_IP1; Homo sapiens; RIP-Seq
Total RNA was extracted using TRIZOL.  1 μg of RNA for each sample was processed with RQ1 DNase to remove DNA. VAHTS mRNA capture beads were used to extract the mRNAs. Double-stranded cDNA was created from fragmented RNAs,After end repair and tailing,they were ligated to VAHTS RNA Multiplex Oligos Set 1 for Illumina. Prior to sequencing, the ligated products underwent amplification, purification, quantification, and storage at -80 °C. Strand-specific sequencing is possible because the second cDNA strand, which is designated with dUTP, is not amplified. cDNA libraries were prepared with the KAPA RNA Hyper Prep Kit (KK8544, Roche, Shanghai China) according the manufacturer's procedure. For high-throughput sequencing, the libraries were prepared following the manufacturer's instructions and applied to Illumina NovaSeq Xplus system for 150 nt paired-end sequencing. fRIP-seq