Chromosome-level assembly of Pilocarpus microphyllus: HiFi from from a greenhouse-grown leaflet specimen
Total genomic DNA from the leaflets was isolated using the MagAttract HMW DNA Kit (Qiagen). PacBio library was prepared using SMRTbell Express Template Prep Kit 2.0 (Pacific BioSciences) and sequenced on an in-house Sequel IIe (Pacific BioSciences) using SMRT Cell 8M Tray (Pacific BioSciences).