GSM8828216: A2.2_7day_1.5nM_dTAG_0day_washout_rep2; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNeasy Mini Kit (Qiagen, 74106), following the manufacturer's instructions, including on-column DNase digestion. Quality control was performed using a DeNovix DS-11 FX spectrophotometer. Libraries were prepared using the QuantSeq 3'mRNA-Seq V2 Library Prep Kit FWD for Illumina (Lexogen, 113.96) and sequencing was performed on an Illumina NovaSeq SP flow cell, generating 100 bp single-end reads.