RNA-seq of marine x freshwater F1 hybrid stickleback: 23wpf, Adductor muscle, 3C
RNA was extracted using the RNeasy Fibrous Tissue Mini Kit (Qiagen). RNA samples were first treated with TURBO DNase (Thermo Fisher Scientific) to remove DNA contaminants. Then rRNA depletion was performed using the QIAGEN FastSelect rRNA Fish Kit (Qiagen) according to the manufacturers protocol. A strand-specific RNA sequencing library was prepared by using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina following the manufacturers instructions (NEB). Libraries were sequenced on the Illumina NovaSeq X Plus according to manufacturer's instructions using a 2x150 PE configuration.