Nanopore sequencing of Acinetobacter baumannii:MC47
Sample libraries were prepared using the PCR-free Oxford Nanopore Technologies (ONT) Ligation Sequencing Kit (SQK-NBD114.24) with the NEBNext Companion Module (E7180L) to manufacturers specifications. No additional DNA fragmentation or size selection was performed. Nanopore sequencing was performed on an Oxford Nanopore a MinION Mk1B sequencer or a GridION sequencer using R10.4.1 flow cells in one or more multiplexed shared-flow-cell runs. Run design utilized the 400bps sequencing mode with a minimum read length of 200bp. Adaptive sampling was not enabled. Guppy1 (v6.4.6) was used for super-accurate basecalling (SUP), demultiplexing, and adapter removal (dna_r10.4.1_e8.2_400bps_modbases_5mc_cg_sup.cfg). No quality trimming has been performed.