GSM8827281: ARPE-19 infected 6hpi rep2; Homo sapiens; RNA-Seq
Total RNA was isolated using RNAeasy columns (Qiagen) and treated with Turbo Dnase (Thermo) prior to library preparation. RNA was quantified and quality-controlled using an Agilent TapeStation 4200. Sequencing libraries were prepared by the UIC College of Medicine Genomics Research Core Facility using a CORALL Total RNA-seq Kit with Ribosomal RNA depletion (Lexogen) and sequenced on an Illumina NovaSeq DNA sequencer in paired-end, rapid mode (50 bp x 2).