GSM9313465: Grade 4 glioblastoma primary tumor sample; Homo sapiens; RNA-Seq
Single nuclei suspensions were prepared from fresh frozen tissue using the Chromium Nuclei Isolation Kit (10X Genomics). Libraries were prepared using the 10X Genomics Chromium Controller on Chip J with expected recovery of 3,000 nuclei. cDNA was amplified and enriched for long fragments. Final libraries were pepared using the SQK-LSK14 ligation sequencing kit (Oxford Nanopore Technologies).