GSM9139390: CMT167R cells_CON; Mus musculus; RNA-Seq
Dissociation used gentleMACSTM Dissociator with Tumor Dissociation Kit (Miltenyi Biotec, Cat. No. 130-096-730), followed by 70-µm filtration. The cell suspension was loaded into Chromium microfluidic chips with 3' v3.1 chemistry and barcoded with a 10× Chromium Controller (10XGenomics). RNA from the barcoded cells was subsequently reverse-transcribed and sequencing libraries constructed with reagents from a Chromium Single Cell 3' v3.1 reagent kit (10XGenomics) according to the manufacturer's instructions. Sequencing was performed with Illumina  NovaSeq 6000  according to the manufacturer's instructions (Illumina).