DNA-seq of HIV proviral DNA from: TSG21N01-S019-PBMC
DNA was extracted from the cells. Two separate  primer pairs spanning gagpol and env genes were used in order to generate 4510 and 3228 base pair amplicons respectively. The amplicons were mixed in equilolar concentration for library preparation. Amplicons were prepped for Illumina-based deep sequencing using the QIAseq FX DNA library kit and the manufacturers instructions.