GSM9312127: HEK293T-CRAMP1-HA-dTAG 24hr treatment; Homo sapiens; RNA-Seq
Trizol Totla RNA was ribodpeletd using NEBNext rRNA Depletion Kit V2 (NEB Cat. E7400).  After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Sequencing was conducted by the NUSeq Core facility.