GSM9351519: cac1Δ::CAC1, glucose repletion, biol rep 1; Cryptococcus neoformans; RNA-Seq
Briefly, 10 ml cultures for five strains (wild-type H99, cac1Δ, cac1Δ::CAC1, cac1Δ::CAC1PP2CΔ, cac1Δ::CAC1ACCΔ) were grown in triplicates in two media conditions, YNB and YNB + 2% glucose. Cells were harvested, snap-frozen, and then lyophilized. The dry cell biomass was weighed and 0.05 g equivalent cells for each sample were used for RNA extraction using the mirVana™ miRNA Isolation Kit (Life Technologies). The RNA samples were quantified using Qubit and submitted to the Duke Sequencing and Genomic Technologies core facility. The library preparation was performed to select and sequence the PolyA mRNA pool (mRNA-seq). The libraries were then sequenced on Illumina Novaseq X Plus to obtain 150-bp paired-end reads.