small RNA sequencing of PA keratitis corneal buttons
miRNA libraries were prepared from 1 g total RNA isolated from the corneal button samples using the QIAseq miRNA Library Kit (Qiagen, Cat. No. 331502) following the manufacturers instructions. Amplified products were size-selected (150170 bp, corresponding to ~22 nt miRNAs) from 6% TBE gels, eluted and quantified using an Agilent 4150 Tapestation and Qubit 3.0 fluorometer. Libraries were sequenced on the Illumina HiSeq 2000 platform.