GSM8993724: E67_knee_spatial01; Homo sapiens; OTHER
Fresh knee tissues were dissected, flash frozen in OCT, sectioned at 10um, permeabalized, and follwed the 10X Genomics spatial Visium HD manufactureres protocol. cDNA was made, amplified and the DNA was purified using Ampure beads. Libraries were constructed following 10X Genomics visium manufactures protocols. Quality was checked using the tapestation, libraries were pooled and eventually sequenced using the NovaSeq X.