BM_S.T_72h_Batch2
Mice were administrated with S4U (1% of body weight) through tail vein injection. 2 h later, mice were euthanized. Femurs and small intestines were collected. For collecting BM cells, muscles from the femurs were removed and epiphyses of the bones were cut off. BM cells were flushed out from the femurs using a 12 cc syringe filled with ice-cold RPMI culture medium. Red blood cells were lysed with red blood cell lysis buffer (eBioscience) and BM cells were washed twice with ice-cold PBS. For collecting the whole epithelial cells and immune cells in intestines, intestinal tissues were opened longitudinally and Peyers patches were removed, and then were washed four times with ice-cold PBS to remove intestinal contents and mucus. Tissues were cut into small pieces and then incubated in digestive buffer (SCelLiVe Tissue Dissociation Kit, Singleron Biotechnologies) at 37C with shaking at 100 rpm. Samples were vigorously pipetted every 5 min. 30 min later, the single-cell suspension was passed through a 40 m filter. Red blood cells were lysed, and cells were washed twice with ice-cold PBS.