RNA-seq of induced mitochronrial import stress and neurodegeneration in Drosophila
RNA-seq of induced mitochronrial import stress and neurodegeneration in Drosophila
For each condition, 15 brains from wandering third instar larvae were dissected and pooled per biological replicate from 3 independent crosses. Brains were transferred into 1.5 ml tubes containing 250 Âµl peqGOLD TriFast (peqlab) and homogenised.  RNA was isolated according to manufacturer's instructions, digested with DNase with subsequent purification with acid phenol.  After integrity check with the Bioanalyzer RNA Pico Chips (Agilent), Illumina-compatible RNA libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) with the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). 