GSM9236025: SETDB2-up group-3; Mus musculus; RNA-Seq
Total RNA was isolated from tissues with TRIzol. Before library construction, total RNA was subjected to mRNA enrichment by poly-T-oligo-attached magnetic beads according to the manufacturer's instructions. Strand-specific RNA libraries were subsequently prepared via the NEBNext® Ultra™ RNA Library Prep Kit (Illumina). The libraries were pooled in equimolar amounts and sequenced via paired -end 150 bp reactions on the Illumina NovaSeq 6000 platform.