GSM9679662: Patient 43, endometrium, healthy control; Homo sapiens; RNA-Seq
Total RNA was extracted from preserved endometrium biopsies using the Monarch® Total RNA Miniprep Kit (New England Biolabs, Ipswich, MA, USA) following the instructions given for extraction from tissues (25-30mg of the sample). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers. Then the second strand cDNA was synthesized using dUTP, instead of dTTP. The directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification.