Single nuclei RNA-seq and snATAC-seq multiome performed in WT and Dhx36 conditional KO hearts at postnatal day 7 (PD7)
Single nuclei RNA-seq and snATAC-seq multiome performed in WT and Dhx36 conditional KO hearts at postnatal day 7 (PD7)
Whole hearts from 7-day-old PD7 wild-type (WT) (Dhx36f/f;Tnnt2 wt/wt) and mutant (Dhx36f/f;Tnnt2 cre/wt) mice were dissected, chopped into several pieces, snap-frozen and stored in liquid nitrogen. On the day of the experiment, wild-type and mutant hearts were pooled (two each), and nuclei were isolated using the Chromium Nuclei Isolation Kit with RNAse inhibitor (10X Genomics; #PN-1000494). Nuclei were captured in the Chromium Controller (10X Genomics) and processed with the Chromium Next GEM single cell Multiome ATAC+Gene Expression (GEX) Kit (10X Genomics; #PN-1000285) ATAC and GEX libraries from the same pool of pre-amplified Tn5 transposed DNA/cDNA