GSM9351391: Rat facial nerve tissue following injury and C-Gel-light treatment 3; Rattus norvegicus; RNA-Seq
Total RNA was extracted from samples using TRIzol reagent. RNA concentration was measured using NanoDrop. The integrity of RNA was assessed by agarose gel electrophoresis. Sequencing libraries were constructed following the standard Illumina protocol. Briefly, mRNA was enriched from total RNA using oligo(dT) magnetic beads, fragmented, and reverse-transcribed into double-stranded cDNA. After end repair, A-tailing, and adapter ligation, the cDNA fragments were PCR-amplified. Final library quality and size distribution were validated using Qubit and an Agilent Fragment Analyzer.