RNA-Seq of Ensifer adhaerens
RNA was extracted from samples, followed by quality assessment using spectrophotometry and electrophoresis. Ribosomal RNA (rRNA) was depleted to enrich mRNA. The remaining RNA was fragmented and reverse-transcribed into double-stranded cDNA. cDNA underwent end repair, A-tailing, and adapter ligation, then fragments were size-selected. Contaminating strands containing uracils were digested, and the library was amplified by PCR. Final library quality and quantity were checked before pooling libraries for Illumina sequencing according to desired data output.