GSM8894785: DKC1 3; Homo sapiens; RNA-Seq
RNA was extracted using TRIzol reagent following the manufacturer's protocol. RNA-ligase-mediated 3`- RACE was performed by ligating a 5`- adenylated and 3`- blocked RNA linker to DNase-treated RNA using T4 RNA Ligase 2 (truncated KQ). Ligated RNA was purified and subjected to reverse transcription using a universal RT primer, followed by PCR amplification with hTR-specific primers (see Supplementary Table 1). Amplified products were purified and used for library construction with the TruSeq Nano DNA LT Library Prep Kit (Illumina), following the manufacturer's instructions.