Illumina NovaSeq 6000 paired end sequencing
Single-cell libraries were prepared with the 10x Genomics Chromium Single Cell 5â€² V(D)J workflow (Next GEM v2). Following cDNA amplification, TRG/TRD CDR3 regions were enriched by a two-step PCR using custom reverse primers, then indexed, pooled, and sequenced paired-end on an Illumina NovaSeq 6000.