GSM9682350: Kitra-25nM minn-4h-R1; Homo sapiens; RNA-Seq
RNA was extracted and purified from flash frozen tumors and cells using a Qiagen RNeasy Plus kit (Cat. 74134) High quality RNA (RIN >7) was divided into duplicate from which 150bp paired-end, rRNA-depleted libraries were made using the Illumina TruSeq RNA library Prep Kit (Illumina, CA, USA). Libraries were quantified using the KAPA Library Quantification kit (KAPA Biosystems, MA, USA), multiplexed, clustered onto flowcells, and then sequenced using an Illumina HiSeq 4000 sequencer (or equivalent platform) by GENEWIZ (Azenta, NJ, USA).