GSM8893245: MDA PCa 189-1, scRNAseq; Homo sapiens; RNA-Seq
Fresh tissue from  PDXs MDA PCa 177-B and MDA PCa 189-1PDXs was harvested and tissue dissociation to single cell suspension was performed using the enzymatic mix solution Accumax (Thermofisher, Cat. No.: 00-4666-56) for 20 min at 37°C under rotation. The cells were washed twice with PBS through cell strainer (70 μm) and centrifuged at 1,500 rpm for 5 min. The single cell suspensions were diluted in PBS with 0.4% BSA and transferred to the MDACC ATGC facility of MDACC for scRNA library processing using the Chromium Single Cell Gene Expression 10X Genomics protocol Library was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics).