DNA-seq of raw river water: RAW1
DNA extracted from riverine water. Water was filtered with 0.45 m PES filters. Cells from filters were resuspended in 1X PBSTween 20. Extraction included a modified DNeasy PowerLyzer PowerSoil protocol, and overnight precipitation. Libraries prepared with the Oxford Nanopore Native Barcoding Kit 24 V14 (SQK-NBD114.24) and sequenced on a MinION Mk1B with R10 flow cells.