16S V3-V4 amplicon sequencing of NA compost at day 22 replicate 3
Total genomic DNA was extracted from compost samples using the FastDNA SPIN Kit for Soil (MP Biomedicals, Santa Ana, CA, USA). The V3-V4 hypervariable region of the bacterial 16S rRNA gene was amplified using primers 341F (5'-CCTACGGGNGGCWGCAG-3') and 806R (5'-GGACTACHVGGGTATCTAAT-3'). PCR products were purified, quantified, pooled in equimolar amounts, and sequenced on an Illumina NovaSeq 6000 platform generating 2 x 250 bp paired-end reads by Gene Denovo Biotechnology Co., Ltd. (Guangzhou, China).