Soil microbial community from the tropical dry forest Deciduous Pacific Forest of the Equatorial coast (DPF), Ecuador, during the rainy season, plot P2, transect T1
Ribosomal V4 region amplified with 515F-Y (Parada et al., 2016) and 926R (Quince et al., 2011), and the Illumina adapter overhang in both primers for a dual-barcoding approach. Amplification using PCRBIO HiFi Polymerase (PCR Biosystems Ltd) under the following conditions: 95C for 3 min, followed by 28 cycles of 95C for 20 s, 60C for 20 s and 72C for 30 s, and final elongation step at 72C for 5 min. PCR products were purified with Agencourt AMPure XP Beads (Beckman Coulter Inc.), and libraries prepared with the Nextera XT DNA Library Preparation Kit (Illumina Inc.).