GSM9643484: iUB organoid, GDNF++, rep3; Homo sapiens; RNA-Seq
Day 12 iUB or day 11 iUB-optoRET organoids were extracted from Matrigel droplets using 600 µl of chilled Cell Recovery Solution (#354253, Corning) on ice for 20-30 min and were triturated approximately every 5 min. Organoids from each group were pooled into a single 15 ml conical tube, washed briefly in 1x DPBS, and placed in DNA/RNA shield (#R1100, Zymo). All sample preparation areas were pre-cleaned with RNaseZAP (#R2020, Sigma) before handling. Libraries were prepared using a commercial service (Plasmidsaurus). In brief, total RNA per sample was subject to mRNA capture, reverse transcription, and second strand synthesis. This was followed by tagmentation and Illumina library indexing and amplification.