GSM8827697: NCI-H2030, miR-494-3p AS; Homo sapiens; RNA-Seq
Total RNA was isolated following manufacturer's instructions using RNeasy Mini Kit (Qiagen, 74104) An RNA-seq library was prepared using the NEBNext UltraII Directional RNA Library Prep Kit for Illumina  (New England Biolabs, Ipswich, Massachusetts, USA) according to the manufacturer's protocols.