GSM8995793: KPPC_WT; Mus musculus; RNA-Seq
Pancreatic tumors were harvested immediately after euthanasia cardiac punctured. Isolated fresh tumor tissues were washed with cold PBS, minced and dissociated to single-cell suspensions using enzymatic digestion buffer containing HBSS supplemented with 10% FBS, 2 mg/mL collagenase A (Roche), and 50 μg/mL DNase-I (Roche), for 45 minutes at 37°C with continuous rotation. Digested single-cell suspensions were filtered through 40-μm nylon strainers (Fisher Scientific) and cells were counted with using TC20 automated cell counter (Biorad). Single-cell suspensions were used for scRNAseq. scRNAseq libraries were prepared for sequencing using standard Illumina protocols