Nivolumab-CDRL1 without TdT expression
Genomic DNA was extracted from approximately 510 reprogrammed HEK293T cells using the DNA extraction kit . A two-step PCR strategy was employed for library preparation: the first PCR amplified the variable regions flanking the engineered RSS sites with gene-specific primers using Q5 High-Fidelity DNA Polymerase (16 cycles), followed by a second PCR to append full-length Illumina adapters and sample indices. The purified libraries were quantified, pooled, and sequenced on the Illumina MiSeq platform using 2250 bp paired-end chemistry.