packaging-2
Total microbial DNA was extracted using the OMEGA Soil DNA Kit (M5635-02). The V5-V7 hypervariable region of the bacterial 16S rRNA gene was amplified via PCR with specific primers 799F (5-AACMGGATTAGATACCCKG-3) and 1193R (5-ACGTCATCCCCACCTTCC-3) using ABclonal DNA polymerase. Libraries were constructed with the Illumina TruSeq Nano DNA LT Library Prep Kit, followed by 2250 bp paired-end sequencing on the Illumina NovaSeq 6000 platform with the NovaSeq 6000 SP Reagent Kit (557 cycles).