GSM9682400: HS-SY-II ChIP-seq GLTSCR1, DMSO; Homo sapiens; ChIP-Seq
Cells were crosslinked with 1% formaldehyde for 10 min and quenched with glycine. After lysis, chromatin was sonicated to ~200-500 bp fragments. Immunoprecipitation was performed with antibodies against GLTSCR1 or anti-HA (recognizing HA-tagged SS18::SSX fusion) overnight at 4°C with Protein A/G beads. Beads were washed, eluted, reverse-crosslinked, and DNA was purified for library construction. Matched input chromatin controls were prepared in parallel. ChIP and input DNA were used to construct Illumina sequencing libraries for paired-end sequencing.