Whole genome sequencing of Klebsiella pneumoniae found in rectal carriage of patient #4
Library selection : nanopore sequencing. Genomic DNA (gDNA) was extracted using 45mg of bacterial pellet cultivated overnight in LB. The NucleoSpin Microbial DNA kit (MACHEREY-NAGEL) was used following kit instructions. Complete sequencing and assembly of gDNA from individual strains were performed using Eurofins genomics service employing state-of-the-art Oxford Nanopore Technologies (ONT) long-read sequencing technology.