GSM8604156: SPOCK2_KO_beta; Homo sapiens; RNA-Seq
hPSC derived WT and SPOCK2 KO beta-cell spheroids, were dispersed with Tripsin, washed with 0.1% saponin – 1% BSA – PBS, filtered through 30 μm mesh filter. Afterwards cells were counted using Countess and adjusted to 700-1,200 cells/μl. The scRNA-seq was performed with 10xGenomics platform and Illumina according to the manufacturer's protocol. Libraires were pair-end sequenced with depth of 40,000 reads per cell.