WGS of Hop parent Wye Target: leaf
Whole?genome shotgun sequencing of Humulus lupulus genomic DNA. Libraries prepared with Tecan (formerly NuGEN) Encore Rapid DR Multiplex System after enzymatic fragmentation (Fragmentase, Allegro Targeted Genotyping kit). Libraries amplified (8 PCR cycles) and size?selected to 500800 bp, then sequenced on an Illumina NextSeq 2000 with XLEAP?SBS chemistry to generate paired?end reads (2 x 250bp). Reads were adapter-trimmed.