RNA-Seq of mus musculus
The 16S rRNA gene was amplified from genomic DNA using primers targeting the V3-V4 region (341F/806R). PCR products were cleaned using a commercial clean-up kit and quantified. Indexed adapters were ligated to the amplicons, and libraries were pooled in equimolar concentrations. The pooled libraries were sequenced on an Illumina MiSeq platform using paired-end 2x300 bp sequencing to generate raw FASTQ data for analysis.