GSM8603273: SF3B1-Flag IPseq wt; Homo sapiens; OTHER
HMW extracts with TDNase+RNasaA+RNaseT1+NucA. IP-seq, 11-14 PCR cycles. The reads from these libraries should look as follows:  NNNNabcNNNNxxxxxxxxxxxxxxxxxxxxxx...xxxxxxxxTGAGATCGGAAGAGCGGTTCAG  Where  - NNNN is a four nucleotide degenerate sequence region. The first to these is to ensure high complexity sequence at the beginning, and the second - to serve as a reference for removing PCR duplications.  - abc is a three letter barcode: - xxxxxxxxxxxxxxxxxxxxxx...xxxxxxxx denotes the insert in the read. The insert starts at position 12 (1-based) of the read, and originate from human (HEK293) RNA fragments 28-70 nt in length.  - The TGAGATCGGAAGAGCGGTTCAG sequence is common for all reads and should be immediately downstream of the insert.