Whole genome sequence of Bacillus subtilis isolated from lacchi juice in Bangladesh
Whole genome sequencing of ONE bacterial DNA samples was performed using the Illumina NextSeq 2000 platform, generating paired-end reads with a read length of 151 bp. Data quality check was done using FastQC analysis (Andrews S. 2010), and subsequent adapter trimming and quality filtering were carried out using Fastp v0.23.4 with parameters --average_qual 20 and --length_required 35 (Chen S. et. al. 2018). De-novo genome assembly from the trimmed reads was performed using Unicycler v0.5.0 with a minimum contig length set to 200 bp (Wick RR, et. al. 2017). Finally, genome annotation was conducted using Prokka v1.14.5, following genetic code 11 (Seemann T., et al. 2014).