Gut microbiota 16S rRNA gene sequencing of child with healthy control (sJIA)
16S rRNA gene amplicon sequencing targeting the V3-V4 hypervariable region. PCR amplification was performed using primers 338F (ACTCCTACGGGAGGCAGCA) and 806R (GGACTACHVGGGTWTCTAAT). DNA was extracted with TGuide S96 Magnetic Soil/Stool DNA Kit (Tiangen Biotech, Beijing). A defined proportion of spike-in reference sequences was added before amplification for absolute quantification. Small-fragment libraries were constructed and paired-end sequenced (2x250 bp) on Illumina NovaSeq 6000.