GSM9021625: RPTEC-TERT1 cell KO1, biol rep 2; Homo sapiens; RNA-Seq
RPTEC-TERT1 Control, KO1, and KO2 clones were cultured to 70-80% confluency before RNA extraction with the RNeasy Plus Mini kit (QIAGEN, #74136). 1.0 µg of total RNA was used for the construction of the libraries RNA for RNA-seq libraries was prepared with with the Watchmaker RNA Library Prep Kit with Polaris™ Depletion following manufacturer's protocol