GSM8966757: HCT116_Hx_rep2_S12; Homo sapiens; RNA-Seq
RNA was extracted using the RNeasy Plus Mini Kit (Qiagen) with an on-column Dnase-treatment (Qiagen). Poly-A mRNA was purified using NEBNext Poly-A mRNA Magnetic Isolation Module. 1-4 µl of 1:10 diluted ERCC RNA Spike-in Mix 1 (Ambion, 4456740) was added to each sample. Messenger RNA (mRNA) isolation and library preparation was performed by the US NCI CCR Genomics Core using the NEB Ultra II RNA-seq kit and PolyA (mRNA) selection.