GSM8453005: Spl_D7_75ng#2; Mus musculus; RNA-Seq
Whole tissue was homogenized in Trizol and RNA was extracted with phenol/chloroform extraction, followed by purification with an RNeasy Kit (Quiagen). RNA was diluted in TCL buffer before library construction. Libraries were constructed following the Smart-seq2 RNA-seq library preparation protocol.