GSM9021409: medullart thymic epithelial cell, Aebp2-ko, replicate2, bulk RNAseq; Mus musculus; RNA-Seq
Thymi were isolated and cleaned to get rid of the residual fat and connective tissue, followed by a serial steps of enzymatic digestion with 0.15% mg/ml Liberase TM in RPMI 1640 at 37 °C. For adult thymi, CD45- cells were enriched with anti-CD45 beads and MACS LS columns (Miltenyi) followed by FACS sorting to harvest living CD45-Ter119-EpCAM+ thymic epithelial cells. The enriched total TECs or mTEChi (UEA1+MHCIIhi) were subjected to scRNA-seq, scATAC-seq, Cut&Tag, bulk RNA-seq. Liraries for scRNAseq, scATACseq, Cut&tag, and bulkRNAseq were prepared following the manufactures' instructions described in the paper's method section.