GSM9237685: scrotal testis (C), Chihuahua, age 29 months; Canis lupus familiaris; miRNA-Seq
Total RNA from testicular tissue was isolated using an miRNeasy Mini Kit (Qiagen) according to manufacturer protocol. The concentration of miRNA was measured with Qubit RNA BR Assay Kit (Invitrogen) on a Qubit 2.0 fluorometer (Invitrogen). The purity was analyzed using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific). The RNA integrity number (RIN) values were checked on a 2100 Bioanalyzer instrument (Agilent) using RNA 6000 Nano kit (Agilent). For each sample, 200 ng of total RNA was used for library preparation with NEXTflex Small RNA-Seq Kit v3 (Bioo Scientific).