RNA-Seq of Danio rerio: embryo whole body
Total RNA was extracted from zebrafish tissue using TRIzol reagent according to the manufacturer's protocol. RNA integrity was verified using an Agilent 2100 Bioanalyzer. mRNA was enriched using poly(A) selection and fragmented. First- and second-strand cDNA synthesis was performed, followed by end repair, A-tailing, and adapter ligation. The library was amplified by PCR and sequenced using the BGISEQ MGISEQ-2000RS platform with paired-end 150 bp reads.