GSM8653774: o2R + MSA-oIL2-2; Mus musculus; RNA-Seq
Total RNA was extracted using the Quick-RNA 96 Kit (Zymo Research). mRNA was isolated using poly-T magnetic beads, fragmented, and converted into cDNA using random hexamer primers. After second-strand synthesis, libraries were prepared through end repair, A-tailing, adapter ligation, size selection, amplification, and purification.