GSM9352153: tomatidine treatment; Mus musculus; RNA-Seq
Fixed, single cell suspension was prepared from flash frozen hippocampus using the Chromium Next GEM Single Cell Fixed RNA Preparation kit (10x Genomics) according to the user guide (CG000553, Rev B). Cell dissociation was performed using the gentleMACS Octo Dissociator (Miltenyi). Libraries were constructed using the single-plex Chromium Fixed RNA Profiling kit (CG000477, Rev C). Libraries were sequenced with the Element Biosciences AVITI Sequencing platform using the AVITI 2x75 High Output Cloudbreak Freestyle Kit (Element Biosciences).