Developmental DNA Methylation in the Parasitoid Wasp Nasonia vitripennis
Developmental DNA Methylation in the Parasitoid Wasp Nasonia vitripennis
1,700 embryos, 30 larvae, 10 prepupa, 10 pupa and 10 adults were used for each replicate of the developmental stage samples. DNA for whole genome bisulphite sequencing for each developmental stage of Nasonia vitripennis was extracted using the DNeasy Blood & Tissue Kit (Quiagen) with some adjustments to the standard protocol. Purelink RNase was used for RNA removal. Larval samples were found to be RNA rich, so RNase incubation was increased to 90 minutes. Library preparation for whole genome bisulphite sequencing was performed by BGI Genomics (Hong Kong)