RNAseq of Homo sapiens: female peripheral blood lymphocyte
CD4 T cells were isolated from three healthy donors and co-cultured with autologous lymphoblastoid cell lines (LCLs). LCL-stimulated CD4 T cells with diminished cytotoxic activity (from days 58-66 of co-culture, 4CTL-LT) were sorted using FACSAria II (BD Bioscience) and subjected to RNA sequencing.