GSM8827179: LPS_2; Mus musculus; RNA-Seq
The isolated testis are quickly put into RNA later (Invitrogen, Waltham, Massachusetts, USA) and stored at low temperature. TRIzol (Invitrogen, Waltham, Massachusetts, USA) was used to isolate total RNA from islets according to the manufacturer's protocol. The amount of total RNA was measured with Nanodrop 2000 (Thermo Scientific, Waltham, Massachusetts, USA), and the quality was evaluated by 1% agarose gel electrophoresis, and stained with 4S Red Plus nucleic acid stain (Sango, Shanghai, China). RNA-seq libraries were prepared using the KAPA Stranded RNA-Seq Library Preparation Kit.