soybean inoculated with B bassiana r3
Leaves were collected seven days post-inoculation, surface-sterilized, and immediately frozen in liquid nitrogen for subsequent RNA extraction. Three biological replicates were processed for each condition: control and B. bassiana-treated. Total RNA was extracted from 100 mg of leaves from each condition using Tri Reagent (MRC, USA), followed by a DNA digestion with the TURBO DNA-free Kit (Thermo Fisher, USA). RNA samples were quantified using a NanoDrop spectrophotometer (Thermo Fisher, USA), and their integrity was assessed on a 1% (w/v) agarose gel. High-quality RNA from each sample was sent for RNA sequencing on an Illumina NovaSeq 6000 platform (Macrogen Inc., South Korea). TruSeq Stranded Total RNA with Ribo-Zero H/M/Gold was used to remove both cytoplasmic and mitochondrial rRNA, and cDNA library preparation. About 3.58  109 paired-end reads of 200 bp were generated (168,509,632 pairs derived from the control condition and 189,789,675 derived from the treatment condition).