GSM8893060: UTD macrophage treated; Mus musculus; RNA-Seq
Tumor tissues were extracted on experiment day 13 and processed, stained with anti-CD45 antibody and live CD45+ tumor-infiltrating leukocytes were sorted using BD Aria III sorter. The samples were submitted to the Integrative Genomics Core at City of Hope for library preparation and subsequent RNA sequencing. Around 7,000 cells per sample were captured on a 10xGenomics Chromium controller using a 10X V3.1 Single Cell 3' Solution kit (10xGenomics, Chromium Next GEM Single Cell 3' Regent kits V3.1, Cat. PN-1000268). All protocols were performed following the manufacturer's instructions. Final sequencing libraries were analyzed on a High Sensitivity DNA Chip (Agilent, Cat 5067-4626) to determine the library size. The library concentration was determined with a Qubit High Sensitivity DNA assay Kit (Thermo, Cat. Q32854).