GSM8684486: macro-metastatic niche,replicate 1,scRNAseq; Mus musculus; RNA-Seq
Liver single cell suspension preparation was performed by the laboratory staff of NovelBio Biopharmaceutical Technologies Ltd. Liver lysis was performed as described previously, whole mouse livers were removed, cut into small pieces on ice, and then enzymatically lysed with 1 mg/mL Collagenase II (Worthington) for 30 min on a shaker at 37°C. 70-micron cell filters were sieved and then erythrocytes were lysed in erythrocyte lysis buffer (Miltenyi Biotec), rinsed twice with PBS, and then the MACS Dead Cell Removal Kit (Miltenyi Biotec) was used to further enrich the single cell suspension. Single cell capture was realized using the BD Rhapsody system. Whole transcriptome libraries were prepared according to the BD Rhapsody Single Cell Whole Transcriptome Amplification Workflow and 150 bp paired-end sequencing was performed using the Illumina (Illumina, San Diego, California, USA).