GSM8510330: U251MG cells, Control, ATAC-seq; Homo sapiens; ATAC-seq
RNA-seq: Total cellular RNA was extracted using Trizol reagent (Invitrogen, San Diego, CA, USA). ATAC-seq: Cells were used for ATAC-seq analysis following the manufacturer's protocol (Active motif Inc., Shanghai, China). RNA-seq: The NEBNext Ultra mRNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA) was used for RNA library construction. ATAC-seq: Chromatin was extracted and processed for Tn5-mediated labeling and adapter incorporation using the Nextera DNA Sample Preparation Kit (Illumina, San Diego, CA).