Depletion daily-12w_tumor_Rep2_AB1162
mRNA from cells sorted into capture plates was barcoded and converted into cDNA. The resulting cDNA was pooled and amplified by T7 in vitro transcription. Then, the RNA was fragmented and converted into a sequencing-ready library by tagging the samples with pool barcodes and Illumina sequences during the ligation, reverse transcription and PCR steps. The library's quality was evaluated and concentration was assessed as described in the MARS-seq2.0 protocol41. Primer barcodes and genes were used according to MARS-seq2.0 protocol for both library preparations and qPCR validations.