GSM9022560: [ATAC-Seq] Control_72h_biol rep1; Mus musculus; ATAC-seq
Cells were harvested at 0, 24, 48 and 72h post-treatment and processed according to the Omni-ATAC protocol (Corces et al. Nature Methods 2017) Libraries were constructed as described in Omni-ATAC protocol (Corces et al. Nature Methods 2017). The final libraries were subjected to double size selection according to a published protocol (Library Preparation for ATAC-Sequencing of Mouse CD4+T Cells Isolated from the Lung and Lymph Nodes After Helminth Infection Laura D. Harmacek, Preeyam Patel, Rachel Woolaver, R. Lee Reinhardt, and Brian P. O'Connor) and quality checked using Qubit and Agilent Bioanalyzer by Purdue Genomics Core Facility.