GSM9138243: HT tumor, ST14KO; Homo sapiens; RNA-Seq
Tumors were lysed in QIAzol Lysis Reagent(QIAGEN), and RNA purified with RNeasy Mini Kit (QIAGEN). Total RNA (1 µg) was used for library construction. Poly(A)+ mRNA was isolated using oligo(dT) magnetic beads and fragmented using divalent cations at elevated temperature. First- and second-strand cDNA synthesis was performed using random primers. Double-stranded cDNA underwent end repair, A-tailing, and adaptor ligation. Size selection was performed using magnetic beads, followed by PCR amplification with indexed primers. Library preparation was performed by Genewiz on an Illumina NovaSeq 6000 platform using 2×150 bp paired-end mode.