GSM9428970: WT2; Mus musculus; RNA-Seq
Splenic CD11c+ cells were enriched with CD11c-MACS beads (Miltenyi Biotec), and then cells were labeled with the Mouse Immune Single-Cell Multiplexing Kit (BD Biosciences). Labeled cells have been counted and captured using the BD Rhapsody Single-Cell Analysis System Instrument (BD Biosciences) according to the provided instructions. Whole transcriptome analysis (WTA) library preparation and Sample Tag library preparation were produced following the BD Rhapsody System mRNA WTA and Sample Tag Library Preparation Protocol. The quality and quantity of cDNA libraries were checked using the Agilent Bioanalyzer 2100 (Agilent), and sequencing (150 PE reads aiming for 50.000 raw reads/cell) was done at Novogene (Cambridge, UK) using the Illumina NovaSeq 6000 (Illumina).