GSM8967005: mProxSmallIntestine_A20ZF7_rep6; Mus musculus; RNA-Seq
Intact proximal small intestines were lysed in ice-cold TRIzol using Lysing matrix D (MP Biomedical 116913050-CF) with MP Biomedical FastPrep-24 Homogenizer (1 cycle of 20 s at 4 m/s). Total RNA was isolated from TRIzol using Zymo Research's Direct-zol kit per the manufacturer's instructions. One microgram of total RNA was depleted of ribosomal RNA (NEBNext rRNA Depletion Kit v2, NEB E7405) and subsequently fragmented, reverse-transcribed into cDNA, and amplified into barcoded libraries with NEBNext RNA Library Prep (NEB E7765) using custom barcoded Illumina-compatible primers.