GSM9465722: A549 cells, siControl (NT), 16h Glucose deprivation (biological replicate 1); Homo sapiens; RNA-Seq
RNA was extracted using the PureLink RNA Mini Kit (Invitrogen), following the manufacturer's instructions. 2.5 ug of total RNA was used for construction of sequencing libraries. Total RNA quality was assessed prior to library preparation to ensure suitability for sequencing (OD 260/280 ≥ 2.00; OD 260/230 ≥ 2.00). Libraries were prepared using a poly(A) enrichment, non-stranded protocol and sequenced on an Illumina platform (paired-end, 150 bp), yielding approximately 50 million reads per sample, with ≥80% of bases at Q30.