GSM9353033: Resist26_BFRT_Before; Homo sapiens; RNA-Seq
RNA_seq:Total ribonucleic acid (RNA) was extracted from tissues using the QIAzol Lysis Reagent (Qiagen, Hilden, Germany) followed by silica- membrane purification of total RNA (Qiagen, Hilden, Germany). The quality of RNA was validated by the Agilent RNA 6000 Nano-Kit in Agilent 2100 Bioanalyzer according to the manufacturer's protocol (Agilent Technologies, Waldbronn, Germany). mRNA sequencing was performed in-house. RNA-seq: Input RNA was tested for quantity using the Qubit broad range RNA kit and for quality using Agilent Bioanalyzer 2100. Next, 1000 ng RNA was used for library prep using the Zephyr NGS workstation (Revvity), using the NEXTFLEX® Poly(A) Beads 2.0 and Rapid Directional RNA-Seq Kit 2.0.  The amplified libraries were tested for quality using Agilent Tapestation 2200 and quantity using the Qubit dsDNA high sensitivity kit. Finally, sequencing was performed on a S1 Flowcell using the NovaSeq 6000 S1 Reagent Kit v1.5 on an Illumina Novaseq 6000.