GSM8684864: shJmjd1a plus WT, Day 2, Rep 1; Mus musculus; OTHER
CUT&Tag was performed essentially as described in the original protocol (Kaya-Okur et al. 2019 Nat Commun), using anti-H3K9me2 antibody (CMA317/6D11, Hayashi-Tanaka et al. 2011 Nucleic Acids Res). After CUT&Tag reactions, column-purified DNA samples were PCR-amplified with indexed primers using NEBNext HiFi (New England Biolabs), followed by size selection with AMPure XP Beads (BECKMAN COULTER).