WGS of Influenza A virus: ferret nasal wash
Nasal wash samples from ferrets infected with A/California/07/2009 (H1N1) were subjected to viral RNA extraction and underwent RT-PCR. PCR clean up was performed and libraries were prepared using the Nextera XT DNA Library Preparation Kit by Illumina. Libraries were pooled at equal molarities, and fragment size was determined. Size selection was performed on the pooled library, and the library was normalized and diluted to a final concentration of 14 pM. Sequencing was performed on an Illumina MiSeq using the MiSeq Reagent Kit v3.