16S rRNA amplicon sequencing of soil metagenome: non-rhizosphere soil of Caragana korshinskii
16S rRNA gene amplicon sequencing targeting the V3-V4 region was performed using the primer pair 338F (5'-ACTCCTACGGGAGGCAGCAG-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3'). PCR amplification, library construction, and paired-end sequencing (2  303 bp) were conducted on an Illumina MiSeq platform. All procedures were carried out by Shanghai Majorbio Bio-Pharm Technology Co., Ltd.