GSM8654603: miR-9/9*-124 2; Homo sapiens; miRNA-Seq
Total RNA was extracted using the RNeasy plus micro kit (QIAGEN, USA) as instructed by the manufacturer. RNA sample integrity was assessed on a 2100 Bioanalyzer (Agilent, USA) before proceeding to library preparation. Small RNAs were prepared for Illumina sequencing using TruSeq Small RNA Library Preparation kit (Illumina, USA). Libraries were sequenced on an HiSeq 2200.