WT, cultured with SC-1.2
Total RNA was extracted using RNA extraction kit (Tiangen), and RNA quality was assessed using the Agilent Bioanalyzer 2100. After the samples were qualified, the library was constructed. The main process was as follows: eukaryotic mRNA was enriched using magnetic beads with Oligo (dT); Fragmentation Buffer was added to randomly shear the mRNA; the first and second cDNA chains were synthesized using mRNA as a template, and the cDNA was purified; the purified double-stranded cDNA was then end-repaired, A-tailed and connected to sequencing adapters, and then fragment size was selected using AMPure XP beads; finally, the cDNA library was obtained by PCR enrichment.