GSM8604428: Low allele burden Jak2VF, 4D9; Mus musculus; RNA-Seq
Following termination of WD, aortas were isolated, digested with 2.5 mg/ml liberase (Millipore Sigma, LIBTM-RO), 120 U/ml hyaluronidase (Millipore Sigma, H3506), and 160 U/ml DNase I (Millipore Sigma, DN25) for 45 min at 37°C. Library was constructed using 10x Genomics Single Cell 3' v3.1 protocol with multiplexing. Hashtag antibodies from BioLegend TotalSeq-B were added to label each mouse.