GSM8573513: PEO1 B, PDS 2hr, RNA, Rep2; Homo sapiens; RNA-Seq
RNA was extracted from 3.5 million cells per sample using Qiagen's Rneasy mini extraction kit, using Qiagen's QIAshedder homogenizing columns and and an on-column DNase I digestion steps. Sample quality was assessed on an Agilent TapeStation using the RNA ScreenTape. For A cell lines, libraries were prepared by The Francis Crick Institute Advanced Sequencing Facility using NEBNext Ultra II Directional PolyA mRNA preparation kit.