GSM8655203: A549 cells, ATAC-seq,ZNF296OE-rep1; Homo sapiens; ATAC-seq
A total of 5× 104 cells were harvested, washed twice with ice-cold PBS, and lysed using the Hyperactive ATAC-Seq Library Prep Kit for Illumina (Vazyme TD711) to release nuclei. The nuclei were pelleted by centrifugation at 500 × g for 10 minutes at 4°C and used for downstream ATAC-seq library preparation. ATAC-seq sequencing libraries were prepared using theTruePrep Index Kit V2 for Illumina (Vazyme, #TD202) following the manufacturer's protocol.