Full-Length 16S rRNA Profiling of Fecal Microbiota in Adult Male Mice
Genomic DNA was extracted and used as a template to amplify the full-length bacterial 16S rRNA gene via PCR. Primers were designed based on conserved regions of the microbial rRNA gene. Sample-specific barcodes were incorporated to amplify variable regions (single or consecutive), enabling analysis of microbial composition and diversity. Sequencing was then performed on the PacBio SMRT platform.