RNA-seq of mouse colon macrophage-GBC3-1
Poly(A) enrichment method: selectively captures mRNA by binding the polyadenylated tails with oligo(dT) beads. The enriched mRNA is then fragmented, reverse-transcribed into cDNA, and ligated with sequencing adapters. The resulting cDNA library is amplified by PCR and purified before sequencing on an Illumina platform.