Whole genome sequencing of dye-degrading Priestia megaterium strain AKSKSLAB05 isolated from industrial effluent, Tiruppur, Tamil Nadu, India
Genomic DNA of Priestia megaterium strain AKSKSLAB05, a dye-degrading bacterium isolated from industrial effluent in Tiruppur, Tamil Nadu, India, was subjected to hybrid whole genome sequencing. Short-read sequencing libraries were prepared using the QIASeq FX DNA Library Prep Kit and sequenced on the PacBio Onso platform (2  150 bp paired-end). Long-read libraries were prepared using the Oxford Nanopore Native Barcoding Kit (SQK-NBD114.96) and sequenced on the PromethION24 platform. The hybrid sequencing approach was designed to obtain a high-quality, near-complete bacterial genome assembly.Authors: Arshiya Khan (Department of Bioinformatics, Alagappa University, Karaikudi, Tamil Nadu, India), Dr. Anuraj Nayarisseri (In silico Research Laboratory, Eminent Biosciences - EMBS, Indore, Madhya Pradesh, India), Prof. Sanjeev Kumar Singh (Department of Bioinformatics, Alagappa University, Karaikudi, Tamil Nadu, India).