Whole Genome Sequencing of Staphylococcus haemolyticus strain AIL_BAU_sh26
Genomic DNA was extracted from a pure bacterial culture using standard laboratory procedures. DNA concentration and purity were evaluated to ensure suitability for whole genome sequencing. High-quality genomic DNA was used to construct a paired-end sequencing library following the manufacturers protocol for the Illumina platform. Briefly, genomic DNA was fragmented, followed by end repair, A-tailing, and ligation of platform-specific sequencing adapters. Adapter-ligated fragments were purified and enriched by limited-cycle PCR amplification to obtain the final sequencing library. Library quality and fragment size distribution were assessed prior to sequencing.Sequencing was performed on the Illumina NextSeq 500 platform using a 300-cycle kit to generate paired-end reads. Raw reads were evaluated for quality using FastQC v0.12.1. Adapter sequences and low-quality bases were trimmed using FastP v0.23.4. High-quality reads were assembled de novo using SPAdes v3.15.2 with default parameters. The assembled genome was taxonomically verified using Kraken2 v2.1.3.