GSM9236220: rSVA-III-B; Senecavirus A; OTHER
The CHX-treated cells were lysed with lysis buffer and digested with nuclease. Digested samples were purified with size exclusion chromatography to obtain ribosome footprints. Ribosome protected fragments were size-selected with polyacrylamide gel electrophoresis (PAGE), and subjected to rRNA removal. Then, purified RNA fragments were end-repaired, ligated with 3' adapters and reverse-transcribed to cDNA. The cDNA was PAGE gel purified, circularized, and amplified by PCR. RNA libraries for Ribo-seq were prepared using GenSeq® Ribo Profile Kit following manufacturer's protocols.