RNA-seq of dendritic cells isolated from normoglycemic and hyperglycemic mice
RNA-seq of dendritic cells isolated from normoglycemic and hyperglycemic mice
Cells were extracted from lungs and subsequently sample was enriched for immune cells using CD45 microbeads from Miltenyi, and sorted with flow cytometry. RNA was extracted using Qiagen RNEasy kit according to manufacturers instructions libraries were constructed using SCRB-seq protocol