16S rRNA amplicon sequencing of Cervus canadensis: interdigital skin
Genomic DNA (gDNA) was extracted from tissue samples using the ZymoBIOMICS DNA Miniprep Kit, and cell pellets were subject to mechanical homogenization and DNA purification using the ZymoBIOMICS MagBead DNA/RNA Kit (Zymo Research). DNA samples were prepared for targeted sequencing using the Quick-16S Plus NGS Library Prep Kit and Primer Set targeting V3-V4. The final pooled library was cleaned up with the Select-a-Size DNA Clean & Concentrator. The final library was sequenced on Illumina NextSeq 2000 with a p1(Illumina, Sand Diego, CA) reagent kit (600 cycles). The sequencing was performed with 25% PhiX spike-in.