RNAseq of Homo sapiens: male peripheral blood lymphocyte
CD4 T cells were isolated from three healthy donors and co-cultured with autologous lymphoblastoid cell lines (LCLs). LCL-stimulated CD4 T cells with validated killing activity (from days 23-30 of co-culture, 4CTL-ST) were sorted using FACSAria II (BD Bioscience) and subjected to RNA sequencing.