GSM8684723: 5HT1AR MIA 1 PROX+ FOS+; Mus musculus; RNA-Seq
vHPC were dissected one hour after EPM and pooled 6 animals per sample group per replicate. Nuclei were isolated using the Minute™ Single Nucleus Isolation Kit for Neuronal Tissues/Cells, and stained with anti-PROX1 and anti-cFOS antibodies. PROX1+ FOS- and PROX1+ FOS+ nuclei were sorted for library preparation. Libraries were constructed according to manufacterer protocols by the Weill Cornell Epigenomics Core using the Chromium Single Cell 3' Reagent V3 kit.