RNA-seq of DENV2-infected Huh7a
Mi-Seq analysis of m5C modification in DENV gRNA fragment (nt 1106-1266). Total RNA extracted from Huh7 parental (WT), Huh7 non-targeting cells (NT), or Huh7 NSUN6 KO cells infected with DENV2 NGC. In addition, total RNA was extracted from virions released from Huh7 NT and NSUN6 KO cells. RNA was treated with bisulfite conversion, reverse-transcribed, and amplified with tageted PCR.