GSM8860487: lung cells,old mouse IAV infected 3; Mus musculus; RNA-Seq
Seven days after IAV infection, mice were euthanized, and their lungs were perfused. Whole lungs were collected, fixed and frozen according to the Tissue Fixation & Dissociation for Chromium Fixed RNA Profiling kit (10X Genomics, Pleasanton, CA) and the 10x Genomics Chromium Next GEM Single Cell Fixed RNA Sample Preparation Kit. Dissociation of fixed Tissue and creation of single cell suspension were done according to the manufacturers protocol. Cells were loaded onto the Chromium X (10X Genomics), then the Mouse Transcriptome 10x Genomics Chromium Fixed RNA Kit, was used to generate cDNA libraries. All samples were handled so that approximately 10,000 cells were captured as 10X Genomics Gel Beads-in-emulsion (GEMs). Barcoded libraries were sequenced on an Illumina NovaSeq X Plus 25B PE300 instrument using the 10X genomics' suggested cycling conditions.