small RNAseq of Heterocephalus glaber: adult kidney control 2
Kidney samples were sent to Canadas Michael Smith Genome Sciences Centre, Vancouver (BC, Canada) for Small RNA RNA-Seq analysis. To ensure high-quality RNA was used for library construction of the miRNA library, an Agilent Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA) was used to measure RNA concentration, quality, and integrity. Small cDNA libraries were constructed, and quality was then assessed on the Agilent Bioanalyzer 2100 system. Sequencing was carried out using an Illumina NextSeq 2000 platform.