GSM9236380: Spleen: CM + NTCI, biol rep 2; Mus musculus; RNA-Seq
spleen was disrupted in lysis buffer on ice using a Dounce hand homogenizer. Total RNA was isolated using NucleoSpin RNA Plus kit (Macherey-Nagel, Germany) according to the manufacturer's instructions. RNA concentration and purity were determined using a NanoDrop One spectrophotometer (Thermo Fisher Scientific, Hampton, NH). RNA integrity numbers (RIN) were measured using TapeStation system (Agilent Technologies, Santa Clara, CA) total RNA was processed into Stranded mRNA (NEB) Library folowing manufacturer's protocols