GSM9350737: KOPSO_2; Mus musculus; RNA-Seq
Freshly collected skin tissues were rinsed with ice-cold PBS and minced into small pieces on ice using sterile scissors. The tissues were then dissociated using a BD tissue dissociation kit, and the resulting cell suspension was filtered through a 70-μm cell strainer. Cell viability was assessed by trypan blue staining, and the concentration of live cells was adjusted to the desired range. Finally, the cells were loaded onto BD Rhapsody Cartridge Kits to facilitate the formation of single-cell magnetic beads within the microwells according to manufacturer instructions. BD Rhapsody Whole Transcriptome Analysis (WTA) Amplification Kit was used to construct single-cell libraries.