GSM8508933: HCT116-Control,8h-1; Homo sapiens; RNA-Seq
The HCT116-AID2 cell lines were induced by 1 µg/ml of doxycycline for 24h, and wereadded or not added (as the control) to 10 µM 5-Ph-IAA (MCE, HY-134653) for several hours. Then transferring cultures to fresh medium supplemented with 10μg/mLactinomycin D (HY-17559, MCE). Samples were harvested at time points of 0, 4, 8 and 12h followingactinomycin Dtreatment. Three replicates were collected at each time point.To determine decay rate of RNA, 500ng HCT116-AID2 cellRNA and 50ngNeurospora RNA were input RT reaction. For tested genes, random RT primers were employed in RT reaction. the VAHTS Universal V8 RNA-seq Library Prep Kit (Vazyme, NRM605)