GSM9643544: Control heart 3; Mus musculus; RNA-Seq
Total RNA from adult mouse hearts were extracted by TRIzol (Invitrogen) with glass dounce homogenizer (Active Motif). For sequencing library preparation, mRNA was converted into complementary DNA (cDNA) via reverse transcription with poly(dT)VN primers and underwent  second-strand synthesis, followed by tagmentation, library indexing, and PCR amplification.