Stool sample of allo-HCT patient shotgun sequenced
After PicoGreen quantification and quality control by Agilent BioAnalyzer, 100 ng of genomic DNA was used as input to prepare sequencing libraries using the KAPA Hyper Prep Kit (Roche 07961901001) with 8 cycles of PCR. Samples were run on a NovaSeq 6000 in a PE100 run, using the NovaSeq 6000 S4 Reagent Kit (X00 cycles) (Illumina). The average number of read pairs per sample was 52 million.