Single Cell VDJ and Gene expression sequencing of HIV antigen specific B cells of Rhesus macaque at Week 93
GEM-X Single Cell 5 Reagents Kit with Feature Barcode Technology were used. For VDJ libraries, cDNA was amplified using in-house Rhesus macaque immunoglobulin primers.  The VDJ, GEX, and CSP libraries were each uniquely indexed: Dual Index Plate TT Set A for VDJ and GEX libraries, and Dual Index Plate TN Set A for CSP libraries, to allow for demultiplexing post-sequencing