GSM9237393: Sol_12M_DMD20; Rattus norvegicus; RNA-Seq
Total RNA extraction was performed from muscle lysates using the RNeasy Plus kit (Qiagen). Total RNA concentration was quantified using a Nanodrop spectrophotometer (ND8000 Labtech, Wilmington Delaware). The RNA quality was evaluated using an Agilent RNA 6000 Pico Kit on a 2100 Bioanalyzer instrument (Agilent Technologies) and Illumina RNA seq was performed on three replicates of each selected clone. The sequencing libraries were prepared using the TruSeq Stranded Total RNA Library Prep Kit (Illumina) and sequenced according to the Illumina NovaSeq™ 6000 protocol.