Yerba mate rhizosphere metagenome
Rhizosphere substrate samples were collected from clonal plants, and total DNA was extracted using the Quick-DNA Fecal/Soil Microbe Kit (Zymo Research). DNA quality was assessed by agarose gel electrophoresis. The V3V4 region of the 16S rRNA gene was amplified using primers 341F and 805R containing Illumina Nextera adapter sequences, following the manufacturers protocol. Amplicons were quantified using the Quantus DNA Kit (Promega), pooled in equimolar concentrations, and sequenced on an Illumina NextSeq 1000 platform using paired-end chemistry (2  300 bp).