BG30TP
DNA of samples was extracted using a QIAamp Fast DNA Stool Mini Kit, parasite 18S rRNA marker amplicons were PCR-amplified from pooled fecal DNA using taxon-specific primer panels. After the DNA samples pass quality control, they are randomly sheared using a Covaris ultrasonicator, followed by end repair, A-tailing, adapter ligation, fragment selection, PCR amplification, and purification to complete the entire library preparation process.