PacBio HiFi sequencing of Bear Paw Lake, AK threespine stickleback
High molecular weight (HMW) DNA was extracted from blood using phenol chloroform extraction. HMW DNA was sheared with Megaruptor 3 (Diagenode, B06010003 & E07010003) using settings 28/31 or 28/30 (depending on original length distribution) and used to generate PacBio HiFi libraries via the SMRTbell Prep Kit 3.0 (PacBio, 102-182-700) using barcoded adapters (PacBio, 102-009-200). Size selection was performed with Pippin HT using a high-pass cutoff of 10-17 kbp (Sage Science, HTP0001 & HPE7510). The library was sequenced on the Revio platform on SMRT Cells 25M with Revio Chemistry V1 with Adaptive Loading and 30-hour movies.