GSM8655085: Set1_h2a_Input_rep2; Arabidopsis thaliana; ChIP-Seq
Seedlings were harvested and fixed with the buffer containing 0.4 M sucrose, 10 mM Tris-HCl (pH 8.0), 1 mM EDTA, 1% formaldehyde, and 1 mM PMSF.Chromatin was sonicated into DNA fragments around 200~500-bp in size using lysis buffer containing 50 mM HEPES (pH 7.5), 150 mM NaCl, 1 mM EDTA, 1 mM PMSF, 1% SDS, 0.1% Na deoxycholate, 1% Triton X-100 and Protease Inhibitor cocktail. For sequencing of H2A, H2A.Z, and H2A.W, the libraries were prepared using the VAHTS Universal DNA Library Prep Kit (ND607; Vazyme, Nanjing, China) and sequenced on an Illumina NovaSeq 6000 instrument via NEO-BIO (Shanghai, China).