RNAseq-dCas9a_IAP_morula
Total RNA was extracted using a TRIzol-based method. For qPCR, cDNA was synthesized with ReverTra Ace (Toyobo) and oligo-dT primers (Takara), and quantitative amplification was conducted using Premix Ex Taq (Takara). mRNA libraries were constructed using the VAHTS mRNA-seq library preparation kit suitable for the MGI platform (Vazyme). High-throughput RNA sequencing was performed on the DNBSEQ-T7 platform (MGI Tech) with paired-end 150 bp sequencing mode.