GSM9312337: CTRL, iMNs, line 2; Homo sapiens; ATAC-seq
Approximately 50,000 nuclei were collected, washed, and incubated in Tagmentation buffer with 5 µl of Tn5 transpose mix (TruePrep DNA Library Prep Kit V2 for Illumina, TD501, Vazyme) at 37°C for 30 min. The fragmented DNA was purified using VAHTS DNA Clean Beads (#N411, Vazyme). The DNA fragments were amplified with TruePrep DNA Library Prep Kit V2 for Illumina (TD501, Vazyme). The universal adapter primer and a barcoded primer (TruePrep Index Kit V2 for Illumina, TD202, Vazyme) were incorporated into the PCR products.