GSM8458121: Fungus cells, Deletion_MtFKH1, Avicel medium, rep2; Thermothelomyces thermophilus ATCC 42464; RNA-Seq
Total fungal RNAs were extracted from fresh mycelia harvested from 2% (w/v) Avicel media through vacuum filtration. Total RNAs were isolated using an RNA extraction kit TransZol Up (TransGen Biotech, Beijing, China) following the manufacturer's protocols, and RNA extracts were assessed by electrophoresis and quantified using the NanoDrop 2000 Spectrophotometer (Thermo Scientific, Waltham, MA, USA). After total RNA was obtained, the mRNA for each sample was enriched by Oligo (dT) beads and reversely transcribed into cDNA via the aid of NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA).