Whole mitochondrial genome sequencing of Sus scrofa: Oocyte from Pig 17.3
Genomic DNA was extracted from total DNA was extracted from Sus scrofa oocytes using the QIAamp DNA Micro Kit (QIAGEN). Four overlapping mtDNA amplicons, size 7262 bp, 3141 bp, 3235 bp, and 3657 bp, were amplified separately using primer Sus1 (Fragment1F=GTTTAACGGCCGCGGTATTC, Fragment1R= TGATTACGTGCAACCCGTGA), Sus2 (Fragment2F=TCACGGGTTGCACGTAATCA, Fragment2R= AATGTCTCCGATGCGGTTGT), Sus3 (Fragment3F= CAGACCGTAGAACTCTCTCTTAGC, Fragment3R= GAACGGGTGTTCTACGGGTT), and Sus4 (Fragment4F= CGTTCAATTCCTAATAAACTAGGTGG, 4= CCTTATTTGGAGAACAAGTGATTATGC), respectively. The amplicons were pooled, and the library was prepared using Prep-M library preparation with 12 amplification cycles. Four overlapping mtDNA amplicons, size 7262 bp, 3141 bp, 3235 bp, and 3657 bp, were amplified separately using primer Sus1 (Fragment1F=GTTTAACGGCCGCGGTATTC, Fragment1R= TGATTACGTGCAACCCGTGA), Sus2 (Fragment2F=TCACGGGTTGCACGTAATCA, Fragment2R= AATGTCTCCGATGCGGTTGT), Sus3 (Fragment3F= CAGACCGTAGAACTCTCTCTTAGC, Fragment3R= GAACGGGTGTTCTACGGGTT), and Sus4 (Fragment4F= CGTTCAATTCCTAATAAACTAGGTGG, 4= CCTTATTTGGAGAACAAGTGATTATGC), respectively. The amplicons were pooled, and the library was prepared using Prep-M library preparation with 12 amplification cycles. Sequencing was performed on Illumina NovaSeq X 10B Lane, 300 cycles using 150bp paired end sequencingn cycles. Sequencing was performed on Illumina NovaSeq X using 150bp paired end sequencing.