Male-HDM-Smallintestine-2
DNA extracted using Qiagen DNeasy Blood & Tissue Kit. The extracted DNA was sent to Novogene for 16S V3V4 microbial amplicon sequencing, generating 30,000 paired-end 250 bp raw reads per sample. After the DNA sample passed QC, amplification primer used to amplify, and purify the DNA through gel electrophoresis. The obtained fragments were end repaired, A-tailed and ligated with Illumina adapter. The fragments with adapters were PCR amplified, size selected, and purified. Quantified libraries pooled and sequenced on Illumina NovaSeq 6000 platform. The  data was transformed to Sequenced Reads, known as Raw Data. Raw data recorded in a FASTQ file.