GSM9139364: Midbrain organoid Line 11 Mock infection 28dpi biol rep 3; Homo sapiens; RNA-Seq
Five organoids were pooled per line per batch, and RNA was extracted using the QIAshredder (Qiagen, 79654) and the RNeasy Mini kit (Qiagen, 74104) after 28 dpi following manufacturer's protocol A total of 750 ng of RNA was used for library preparation using TruSeq stranded mRNA library preparation kit (Illumina, 20020594) as per the protocol provided. The libraries were quantified using Qubit dsDNA HS assay kit (Thermo Fisher Scientific, Q32851) and the size distribution was determined using Agilent 2100 Bioanalyzer (RRID:SCR_019389)