GSM8453079: ZAP+/+ C57BL/6 mouse spleen, poly I:C-treated, 6h, biol rep 1; Mus musculus; RNA-Seq
Extracted spleens were washed in cold PBS and submerged in RNAlater preservation solution. Samples were kept at -80˚C until further processing. Spleens were then thawed and homogenized in a solution of TRIzol reagent and ultrapure water (3:1 ratio). Total RNA was extracted according to manufacturer's guidelines. RNA quality was assessed by nanodrop and independently by Novogene Corporation Inc. Ribosomal RNA was removed from total RNA samples, followed by cDNA synthesis through reverse transcription. RNA libraries were prepared for RNA sequencing by Novogene following Novogene's standard protocols.