Whole genome sequencing of Pseudomonas inefficax MG-2
DNA concentration was measured with a NanoDrop 2000c Spectrophotometer, yielding 500 ng/L. DNA fragmentation was conducted using Covaris S220. Library construction was carried out with the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs). Quality assessment of DNA fragmentation and library preparation was performed using a 2100 Bioanalyzer (Agilent Technologies) and a high-sensitivity DNA kit (Agilent Technologies). DNA sequencing was executed on the Illumina MiSeq platform (Illumina) with a 301-bp paired-end library.