GSM8457531: CTS2 miRNA (biol rep2); Homo sapiens; miRNA-Seq
Total RNA was isolated from cells using TRIzol (Takara). Total RNA was quantified and qualified by Agilent 2100/2200 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA).1.1 ug of total RNA was used for the construction of sequencing libraries. MiRNA libraries were prepared for sequencing using standard Illumina protocols