GSM8509654: BY4743 lacking one chromosome II - replicate 1; Saccharomyces cerevisiae; ssRNA-seq
Equal amounts of the mRNA spike ( 2 μl of 20x diluted ERCC RNA Spike-In Mix,TermoFisher per 10 million cells) were added to all samples. Total RNA was extracted using the FAE method followed by additional purification with RNAzol (as described in  Shedlovskiy et al. 2017). Stranded RNAseq libraries were prepared by the Novogene company (DUTP method). For that, messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers. The second strand cDNA was synthesized using dUTP. The library was then subjected to end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification and purification.