GSM9023347: HPAEC, flow group, 15 dyn/cm2 continuous, rep 3; Homo sapiens; RNA-Seq
mRNA was isolated by pooling cells from 3 Luer I slides for each condition using the RNAeasy mini kit (Qiagen), quantified using Nanodrop and evaluated for quality with a bioanalyzer by the UTSW Genomics Core. All samples had a RIN score of 10. 1.5 µg of RNA was used for library construction. Illumina TruSeq® Stranded mRNA Library Preparation Kit (Catalog #20020594) was used for RNA-seq library construction.