GSM9104353: W07G4.3; Caenorhabditis elegans; RNA-Seq
Total RNAs were extracted using RNA Clean & Concentrator-5 (Zymo, R1014), followed by DNase treatment to eliminate genomic DNA contamination. Total RNA was reverse transcribed using Induro® Reverse Transcriptase (NEB, M0681L) with Random Primer Mix. cDNA was amplified using primers covering the SOS splicing events.