GSM8859911: KC_ID1; Mus musculus; RNA-Seq
Bead-bound nuclei isolated from KC-INTACT mice were resuspended in TRIzol. After further purification steps in chloroform and ethanol, samples were loaded onto RNeasy MicroColumn (QIAGEN, 74004). RNA quantification was performed with a Qubit fluorimeter (ThermoFisher Scientific) and RNA integrity was assessed with a Bioanalyzer (Agilent Technologies). The Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus kit was used for the library preparation with 1 to 5 ng of total RNA (depending on availability) as input. Library molarity and quality were assessed with the Qubit and Tapestation (DNA High sensitivity chip, Agilent Technologies). Libraries were sequenced on a NovaSeq 6000 Illumina sequencer for SR100 reads.