RNA-Seq of ventrolateral human POMC neurons from a human male
Post-mortem human male hypothalamic tissue was obtained from the Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary. Tissue blocks were fixed in 4% paraformaldehyde in sterile PBS supplemented with ATA RNA-protective agent and cryoprotected in 20% sucrose containing ATA. Dorsomedial and ventrolateral POMC neurons in the mediobasal hypothalamus were identified by immunostaining using a modified ImmunoLCM-seq protocol, isolated by laser-capture microdissection, and processed for RNA extraction and bulk RNA-seq on the Illumina platform.