16,500 cells were run on the 10X Chromium to partition single cells into droplets containing uniquely barcoded beads and processed for sequencing library preparation using the Chromium Single Cell 5â NextGEM v2 chemistry. Amplified DNA from the cell surface protein Feature Barcodes derived from the TotalSeq-C antibody cocktail was used to construct the Cell Surface Protein library.
UMIGS-Maryland-Genomics Illumina Construction Protocol Details