GSM8654513: Wastewater COVIDseq RUH 240218; wastewater metagenome; OTHER
Using the Wizard® Enviro TNA Kit (Promega Corp., Madison, USA) as a direct capture method, total nucleic acids (TNAs) were extracted and concentrated from 40 ml of 24 h composite samples, resulting in a final volume of 50-100 µl following the manufacturer's instructions Sequencing were prepared using the COVIDseq kit (Illumina) according to manufacturer's instructions, and sequenced on a NextSeq 2000 device using P1 300 cycles flowcells with 2x151 bp. Targeted/amplicons