GSM8653740: MIA PaCa-2, STN1, CUT&Tag; Homo sapiens; OTHER
Indicated cells were digested into singles cells and respended in PBS. 10,000 cells were used, collected by magnetic beads and incubated with primary antibody, secomdary antibody and pA/G-Tn5 according to the protocol of TranNGS CUT&Tag Library Prep Kit for Illumina by Transgenebiotech. CUT&Tag libraries were prepared using TranNGS CUT&Tag Library Prep Kit for Illumina by Transgenebiotech and constructed by PCR amplification. The number of amplification cycles is 11.