GSM8505132: J-Lat 10.6_ INTS12 KO_DMSO_R1; Homo sapiens; RNA-Seq
Cells were lysed with 700 µl of QIAzol (Qiagen, 1023537). Samples were spun through a QIAshredder (Qiagen, 79654) and then frozen at -80°C. Cells were then processed with a miRNeasy kit (Qiagen, 217004), DNase treated with RNase-Free DNase Set (Qiagen, 79254), and then re-processed through the RNeasy Mini Kit (Qiagen, 74104) with the EtOH wash modified to 700 µL for small RNAs. rRNA was removed with the Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus kit (20040525) followed by indexing with IDT for Illumina RNA UD Indexes Set A Ligation (20040553).