RNA-seq of Macrobrachium rosenbergii:tissue mixture
Total RNA extraction, removing ribosomal RNA, constructing the RNA libraries and meta-transcriptomic sequencing were performed. Different libraries were pooled to flowcell and CBOT was clustered and sequenced using the NovaSeq 6000 (Illumina, USA) high-throughput sequencing technology, according to the requirements of effective concentration and target offline data volume (Illumina, USA). Paired-end (PE) reads sequencing (150 bp) of the RNA libraries was conducted. All library preparation and sequencing were carried out by Novogene (Beijing, China).