GuideSeq of K562 edited cells : gRNA20
Cell were nucleofected with the CAS/gRNA and a short ODN that integrates at the cut sites (onTarget and offTargets). Genomic DNA was extracted from edited cells. The ODN-genome junction was amplified by PCR after adaptor ligation . Library was sequenced using a 2x150bp MiSeq run. (see Tsai, S. et al. Nat Biotechnol (2015) for the complete protocole)