GSM9210832: HSC-2 cells, control, rep1; Homo sapiens; RNA-Seq
Total RNA was extracted using the TRIzol Plus RNA Purification Kit (Thermo Fisher Scientific) according to the manufacturer's protocol, including on-column DNase treatment to remove genomic DNA contamination. Full-length cDNA was synthesized and amplified from 10 ng of total RNA using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Bio) following the manufacturer's instructions. Sequencing libraries were prepared via tagmentation using the Nextera XT DNA Library Prep Kit (Illumina) and uniquely indexed with IDT for Illumina DNA/RNA UD Indexes (Illumina).