GSM9351777: PSD017LL, Lower extremities; Homo sapiens; RNA-Seq
RNA was extracted using an miRNeasy Mini Kit (Qiagen, Germany) and stored at −80°C until further processing. For psoriatic samples, both lncRNA and mRNA 100 bp paired-end libraries were constructed using the Hieff NGS™ MaxUp Human rRNA Depletion Kit (rRNA & ITS/ETS), paired with the Hieff NGS™ Ultima Dual-mode mRNA Library Prep Kit at BGI facilities (BGI, Hong Kong, China). Raw data with adapter sequence or low-quality sequences were filtered using SOAPnuke software by BGI.