GSM8964096: HG-P; Homo sapiens; RNA-Seq
Macular and peripheral explants were dissociated into single cells following light treatment using Papain Dissociation System (Worthington Biochem, NJ, USA) according to the manufacturer's protocol.Then The single-cell suspension was combined with barcoded gel beads in a microfluidic chip and run on the Chromium Controller (10X Genomics) to create gel bead-in-emulsion (GEMs) droplets (Chromium Next GEM Single Cell 3ʹ Kit v3.1, 16 rxns PN-1000268, 10×Genomics). The GEMs were incubated, enabling mRNA reverse transcription into cDNA. The barcoded cDNA was then amplified using PCR to construct a library according to users guide of Chromium Single Cell 3ʹ Reagent Kits v3