RNA-seq of Macrothele washanensis:sample2
Total RNA was extracted from the frozen venom glands using TRIzol, and the concentration and purity of the extracted RNA were quantified using a Nanodrop 2000. RNA integrity was checked by agarose gel electrophoresis, and RIN values were determined using an Agilent 2100. The mRNA was then isolated by A-T base-pairing binding with ployA using magnetic beads with Oligo dT. mRNA isolated by enrichment was randomly broken into smaller fragments of about 200 bp, and the mRNA was used as a template for reverse synthesis of one-stranded cDNA by using random primers under the action of reverse transcriptase, followed by two-stranded synthesis to form a stable double-stranded structure. High-throughput sequencing analysis was performed using the Illumina NovaSeq 6000 platform.