GSM9261635: B_GT3.1-1428Q-_10C_Hash; Mus musculus; RNA-Seq
Inguinal lymph nodes were resected from both sides, LNs were mashed through 70uM strainer into R10 with benzonase. Cells were stained with ENV probe(Oligo-hashtag) and then for B cell and congenic markers.  GC B cells with CD45.1 positivity were sorted per mouse and then combined across 16 GEM reactions in 10x chip comptroler. V(D)J and Feature hashtag libraries with indexes were prepared following the 10X Genomics protocol for Single Cell 5' Reagent Kits v2 (Dual Index). Sequencing was performed on NovaSeq 6000 (Illumina).