B0029.24
Bacterial genomic DNA was extracted with the DNeasy Blood & Tissue Genomic DNA Isolation kit (Qiagen, Hilden, Germany), according to the manufacturers instructions. Next-generation sequencing was performed at Fiocruz Platform Network (Oswaldo Cruz Institute, Rio de Janeiro, Brazil) using an Illumina NextSeq 2000 sequencer (Illumina, San Diego, CA, USA). The preparation of libraries for sequencing was carried out using the Nextera DNA Flex kit (Illumina, San Diego, CA, USA) following the manufacturers instructions, and the sequencing run was performed with P2 XLEAP-SBSTM Kit reagents (Illumina, San Diego, CA, USA), with 300 cycles (2 151 base pair paired-end), according to the manufacturers recommendations.