GSM9465307: Model_2 stool microbiome; Rattus norvegicus; OTHER
Total DNA extracted from stool samples using validated microbiome DNA extraction method; DNA quality assessed via Nanodrop and 1.2% agarose gel electrophoresis. PCR amplification of 16S rRNA gene variable regions using primers with sample-specific barcodes; PCR products purified with magnetic beads; libraries prepared using Illumina TruSeq Nano DNA LT kit; final libraries quantified and size-selected using Agilent Bioanalyzer.