Amplicon-seq of environmental seawater samples
Microbial community composition was assessed using a two-step PCR approach targeting the 16S rRNA gene for prokaryotes (including chloroplast-derived photoautotrophs) and the 18S rRNA gene for eukaryotes, followed by Illumina MiSeq sequencing (2  300 bp) at NGI, Uppsala University. For copepod gut content analysis, 16S PCRs were run using the full DNA extract volume, with 65% of samples showing successful amplification and proceeding to indexing.