Trarnscriptome of Ps+Pl communities
Bacterial cultures were harvested by centrifugation at 5000g for 20 min at 4C. Supernatants were discarded and the cell pellets were frozen in liquid nitrogen. Total RNA was extracted using lysozyme and a hot phenol extraction protocol followed by a DNAse treatment, and details are described in the Supplementary Methods. All samples were sent to Eurofins Genomics (Konstanz, Germany) on dry ice, where the rRNA depletion, library preparation, and sequencing were performed.