GSM8685205: HEK293T untransfected replicate 1; Homo sapiens; RNA-Seq
Total RNA was isolated from HEK293T cells 24h after transfection using RNEasy mini kit with in-column DNAse digestion (Qiagen). Reverse transcription was performed from 1µg of RNA with RevertAid First Strand cDNA synthesis kit (Thermo Scientific, K1622) RNA sequencing libraries were prepared KAPA HyperPrep Kit with RiboErase (Roche, KK8562) and sequenced in 100bp paired-end mode with Illumina NovaSeq2 for 55-65M fragments per sample.