Bird metagenome Maracana 1
Biological samples from birds were collected during eco-epidemiological expeditions in the Algodoal-Maiandeua Environmental Protection Area (APA) in the northeastern part of Par state, Brazil, in 2025. For RNA extraction, pooled blood samples were used. The extraction products were standardized to a concentration of 0.2 ng/L, fragmented, and labeled with adapter sequences. The resulting genomic libraries were quantified and sequenced using the paired-end method (2 x 150) on the NextSeq 500 System platform. The sequencing files were demultiplexed using bcl2fastq v.2.20 and subjected to quality control using Fastp v.0.23.4, configured to remove adapter sequences and reads with a Phred base quality < 20 and a length shorter than 50 nt. Contigs were assembled de novo using MEGAHIT v.1.2.9 in its default configuration (k-mers of 21, 29, 39, 59, 79, 99, 119, and 141 nt), identified using DIAMOND v.2.2.2.182 (Blastx mode, e-value of 0.00001), and manually inspected using Geneious v.11.1.5.