GSM9465252: Luc-A549 tumor, 7d 5Gy treatment, rep2; Homo sapiens; RNA-Seq
Total RNA was purified using the RNease Mini Kit with on-column DNase digestion (QIAGEN). RNA quality and quantity were assessed with a NanoPhotometer (Implen Inc: Westlake Village, CA) and cDNA synthesis was performed using SuperScript IV (Invitrogen). 1 ug of total RNA was used for construction of sequencing libraries. RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit following manufacturer's protocols.