Sbre01_E1_Nanopore (CRX634219; CRA010120)
A total amount of 2 microg DNA per sample was used as input material for the ONT library preparations. After the sample was qualified, size-select of long DNA fragments were performed using the BluePippin system (Sage Science, USA). Next, the ends of DNA fragments were repaired and A-ligation reaction were conducted with NEBNext Ultra II End Repair/dA-tailing Kit (Cat# E7546). The adapter in the LSK109 kit was used for further ligation reaction and Qubit 3.0 Fluorometer (Invitrogen, USA) was used to quantify the size of Library fragments. Sequencing was then performed on a Nanopore PromethION sequencer (Oxford Nanopore Technologies, UK) instrument on Nextomics. submitted_instrument_model: OXFORD_NANOPORE PromethION