GSM8966589: ATAC-seq, NIH3T3, MyoDminus, r2; Mus musculus; ATAC-seq
After incubation, cells were collected and washed with PBS. Libraries were prepared by BRB-Seq(Alpern D, et al.) method. Individual samples were reverse transcribed using dT primer with unique cell barcodes. Then replicate were mixed and amplified by PCR. 10nt of UMI followedby 9nt of cell barcode are in read1 and 81nt of RNA insert reads are in read2. ATAC libraries were prepared as previous report (Harada, Maehara, and Handa 2019).