DNA sequencing of 16S rRNA v4 hypervariable region for bacteria: negative control
16S rRNA v4 region was amplified with primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3'). PCR was performed with Phusion Plus PCR master mix for a total of 40 cycles. PCR products were purified with AMPure XP beads, quantified with Qubit, and equimolar PCR products were pooled for sequencing.