Illumina sequencing of Nagleria fowleri: clinical case from Limon
Naegleria fowleri isolates were maintained under axenic culture conditions. Total genomic DNA was extracted from 1  10 trophozoites using the QIAamp DNA Mini Kit (Qiagen, Germany) with RNase A treatment. DNA quality and quantity were assessed using a NanoDrop spectrophotometer. Sequencing libraries were prepared from genomic DNA using the Nextera XT DNA Library Preparation Kit (Illumina). Paired-end whole-genome sequencing (2  150 bp) was performed on an Illumina NextSeq 500 platform at the Biomics Core Facility, Institut Pasteur (Paris, France).