GSM9137745: Pilot DC-TAP-seq for 97 genes in K562 infected with 97 guides in sgOpti-CS vector at MOI9. gRNA; Homo sapiens; RNA-Seq
Cells were collected using centrifugation, washed with PBS+BSA and subjected to droplet formation for DC-TAP-seq or TAP-seq or scRNA-seq using the 10x Genomics v3.1 kit. DC-TAP-seq libraries were constructed according to the protocol detailed in Ray et al 2025 (dx.doi.org/10.17504/protocols.io.3byl4z7drvo5/v1). TAP-seq libraries were prepared according to Schraivogel et al., Nat Methods, 2020 and scRNA-seq libraries with guide capture were generated according to the 10x Genomics protocol.