ASO treatment of DRPLA fibroblasts
ASO treatment of DRPLA fibroblasts
RNA was isolated from passage 15 ASO-treated fibroblasts harvested as cell pellets  Qiagen RNeasy Micro Kit (Qiagen, GmbH, Hilden, Germany) NEBNext Ultra II Directional RNA Library Prep Kit for Illumina\" (NEB #E7760S/L). Briefly, mRNA was isolated from total RNA using the oligo-dT magnetic beads. After fragmentation of the mRNA, a cDNA synthesis was performed. This was used for ligation with the sequencing adapters and PCR amplification of the resulting product. The quality and yield after sample preparation was measured with the Fragment Analyzer. The size of the resulting products was consistent with the expected size distribution (a broad peak between 300-500 bp).