Poly-A selected RNA-seq library from nasal swab collected for the HEROS study
Libraries were made using the KAPA mRNA HyperPrep kit (Roche Sequencing and Life Sciences, Kapa Biosystems; KK8581) using the Beckman Coulter i7 Automated Liquid handler. Samples were normalized for 50ng of RNA for input, fragmented for 2min at 94C, and were amplified using 16 cycles of amplification. Libraries were pooled and sequenced on Illumina sequencing platforms using 150bp paired end reads.