RNA-seq of maize
Total RNA was extracted with the TRIzol reagent from leaves of 70-day-old maize plants grown in pots in the greenhouse in Qingdao. RNA integrity was assessed using the Agilent 2100 Bioanalyzer. RNA-seq libraries were constructed from the DI water and flash graphene spraying plants in two replicates for each line by using the TruSeq Stranded mRNA LT Sample Prep Kit. The four libraries were sequenced separately on the Illumina HiSeq X Ten sequencing platform. Approximately 15 Gbp clean reads were mapped to the Nipponbare reference genome using the HISAT and Bowtie3 tools.