GSM9682331: GlycoRNA from HeLa cells; Homo sapiens; ncRNA-Seq
Total RNAs from the Ac4GlcNAz metabolically labelled and then RNase treated HeLa cells were extracted with a traditional procedure using Trizol reagent. Glycosylated RNA was enriched using the Ac4GlcNAz-based click chemistry and streptavidin magnetic beads as described. Both input and IP RNA were then subjected to PANDORA-seq library preparation using QIAseq miRNA Library Kit.