PPLwtRibo1
Footprints were size-selected through electrophoresis, ligated to a 3'adpater, reverse transcribed. cDNA was circularized. Libraries were then prepared by PCR amplification. Cells were harvested in lysis buffer (20 mM Tris-Cl [pH 8], 150 mM KCl, 15 mM MgCl2, 1% Triton X100, 1 mM DTT, 0.1 mg/mL cycloheximide, supplemented with 1X cOmplete Mini Protease Inhibitor Cocktail).