GSM8723065: HCT116 input; Homo sapiens; ChIP-Seq
The crosslinked chromatin was digested with micrococcal nuclease to the extent that 70% of the chromatin was cleaved to mononucleosomes. Before performing the ChIP assay, the specificity of the anti-H3K4me3 antibody (Millipore Sigma 17-614 Lot# 2871690) was evaluated. ChIP assays with the anti-H3K4me3 antibody and a control rabbit IgG were done to produce input and ChIP fractions from the four cell lines to determine the enrichment of H3K4me3 mononucleosomes associated with the MCL1 exon 1, exon 2, exon 3, and intron 1. ChIP libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit for Illumina. The libraries were sequenced at Genome Quebec using the Illumina HiSeq 4000 PE100 System at 25 million reads.