PSC co-culture day6 11 samples single cell RNA-Seq
PSC co-culture day6 11 samples single cell RNA-Seq
Co-cultures were dissociated to single cells with dispase and TrypLE. PSC were collected with FACS using fluorescent protein mKate-2 Single cells were captured with the Biorad ddSeq system cDNA amplification and library preparation were performed using the Biorad/Illumina Surecell system.