vRNA seq of Orthoebolavirus zairense grown in cerebral organoids for 120 days EBOV epsiolon
EBOV sequence analysis was performed using culture supernatants via reverse transcription with SuperScriptIV Reverse Transcriptase (Thermo Fisher Scientific; #18090010) and subsequent amplification with random primers. A stranded, paired-end library was prepped using the TruSeq RNA exome (Illumina, San Diego, CA, USA; #RS-301-2001) before sequencing on a MiniSeq (Illumina, San Diego, CA, USA; # SY-420-1001) using a MiniSeq High Output Kit 300 cycles (Illumina, San Diego, CA, USA; #FC-420-1003).