GSM8459828: MAF-928, Hypoxia, 72 hours, rep3; Homo sapiens; RNA-Seq
RNA was extracted using Qiagen All DNA/RNA mini prep kit. Quality of extracted mRNA was assessed using DNA Analysis ScreenTape (Aligent Technologies) to ensure sufficient quality for library preparation. Following quality assessment, cDNA libraries were generated using the Universal Plus mRNA-Seq Sample Prep Kit (Nugen). Libraries were then sequenced using the Illumina NovaSeq6000 platform with paired-end reads (2×151). On average 70 million reads were collected for each sample.