GSM8918290: Root +Fe+U 1; Solanum lycopersicum; RNA-Seq
One gram of root tissue was homogenized in liquid nitrogen, and total RNA was isolated from approximately 60 mg of the resulting powder using the Spectrum Plant Total RNA Kit (Sigma–Aldrich) following the manufacturer's instructions. Library preparation followed the Illumina TrueSeq 2.0 protocol, using 2 μg of total RNA per sample (Venuti et al., 2019). Libraries were prepared using the Universal Plus mRNA-Seq kit (Tecan Genomics, Redwood City, CA) according to manufacturer instruction.