GSM8830571: PAAD_O3; Homo sapiens; RNA-Seq
Total RNA of adhered cells was isolated with the Total RNA purification kit (Norgen, Thorold, ON, Canada) and the quantity and integrity of the RNA was evaluated using the QubitTM RNA HS Assay Kit (Invitrogen, Waltham, MA, USA) and Agilent RNA 600 NanoChips (Agilent Technologies, Santa Clara, CA, USA) mRNA stranded libraries were prepared following “TruSeq Stranded mRNA Sample Preparation Guide (Part # 15031058 Rev. E)” using the “TruSeq® Stranded mRNA Library Prep” kit  (Illumina Inc. Cat. # 20020594) and TruSeq RNA CD Index Plate (96 Indexes, 96 Samples) (Illumina Inc. Cat. # 20019792)