GSM9860460: BCC7; Homo sapiens; RNA-Seq
Fresh tumor tissue was minced and enzymatically digested to obtain a single cell suspension. Single living cells were then FACS sorted. The cells were resuspended to reach a target final concentration of approximately 1,000 cells/μl.  ScRNA-seq was performed using the Chromium Next GEM single cell 3' reagent kit v3.1 (10xGenomics) following the manufacturer's protocol with a target number of captured cells of 10,000. Sequencing libraries were prepared after GEM generation and barcoding using the Chromium Next GEM single cell 3'reagent kit v3.1 (10xGenomics) the following manufacturer's protocol.