RNA-Seq of MDA-MB-231 non-targeting control replicate 1
Bulk poly(A)-selected mRNA sequencing of NC_1; non-targeting shRNA control; puromycin-selected. Libraries were prepared by oligo(dT) mRNA enrichment, mRNA fragmentation, random-primed first-strand cDNA synthesis, second-strand synthesis, end repair, A-tailing, adapter ligation, approximately 200-bp cDNA size selection, PCR amplification, and Illumina paired-end 150-bp sequencing. The submitted paired-end FASTQ files are fastp-processed clean reads. Adapter-containing reads, reads with excessive ambiguous bases, and low-quality reads/bases were removed or trimmed before submission.