16S rRNA amplicon sequencing of mouse fecal microbiota
16S V3-V4 region amplified with primers F:CCTAYGGGRBGCASCAG R:GGACTACNNGGGTATCTAAT; sequenced on NovaSeq 6000 PE250. Raw paired-end reads were merged by Novogene using FLASH, then quality filtered and chimera removed. Final merged reads are provided as single-end FASTQ.