GSM8968085: Leaf from family NE72, plant #9; Zea mays; OTHER
DNA was extracted from leaves using Qiagen Dneasy plant mini kit according to manufacturers instructions. DNA from Endosperm samples were extracted using a modified CTAB DNA extraction protocol (see methods). All DNA samples were then sheared to a mean of 1000 bp using a Covaris E220 Evolution. Libraries were constructed for all samples by first ligating a custom Museq Adapter, and then subsequent PCR reactions to selectively amplify genomic regions adjacent to Mu insertions. See Methods for details