sequencea of archaeal
The archaeal project selected the highly variable V4-V5 region of the archaeal 16S rRNA gene for sequencing. PCR amplification uses 16S rRNA V4-V5 region-specific primers: 524F (5-barcode+TGYCAGCCGCCGCGGTAA -3) and 958R (5-YCCGGCGTTGAVTCCAATT -3).