RNA-seq of human lung epithelial A549 cells compared to A549 cells with shBMAL1 mediated knockdown of BMAL1 at two circadian phases
RNA-seq of human lung epithelial A549 cells compared to A549 cells with shBMAL1 mediated knockdown of BMAL1 at two circadian phases
Dishes were washed with PBS and frozen at -80C till RNA extraction.  Cells were grown in DMEM, 10% FCS, P/S, glutamax.  Cells were seeded 24hours before forskolin treatment for synchronisation and then collected 12 and 24hours post FSK.  Quiagen RNAeasy column with DNA clean up column PolyA enrichment for mRNA of all samples. 