GSM9059358: KS_A3; Homo sapiens; OTHER
FFPE treated samples were deparaffinized with investigator placed sections, H&E stained, imaged and decrosslinked. The tissue was permeabilized and 10X Genomics human transcriptome V1 probes were hybridized to the mRNA overnight for KS_A1, KS_A2, and KS_A3. For KS_B1, KS_B2, and KS_B3 and KS_B4 the same process was performed with V2 and spiked KSHV genes (listed in Visium_Human_Transcriptome_Probe_Set_v2.0_GRCh38-2020-A_K2.csv).  The probes were ligated, released from the tissue, captured on the spatially barcoded spots and subjected to extension followed by denaturation per manufacturer protocol.