LVHT-1-C
LVHT samples for single-nucleus RNA sequencing (snRNA-seq) were obtained from explanted recipient hearts of patients who underwent heart transplantation. On ice, myocardial tissue from the left ventricle was carefully dissected into compact and non-compacted layers. In control hearts, tissue was separated into the myocardial wall and trabecular layers. Samples were immediately snap-frozen in liquid nitrogen and subsequently stored at 80C. The time from cardiac explantation to freezing was maintained at less than 30 minutes to ensure RNA integrity. snRNA-seq libraries were prepared using the Chromium Next GEM Single Cell 3 GEM, Library & Gel Bead Kit v3.1 (10x Genomics, 16 reactions) and sequenced on the Illumina HiSeq X Ten platform.