GSM9137374: Parental, rep3; Homo sapiens; RNA-Seq
Total RNA was extracted using the FavorPrep™ Total RNA Isolation Kit (Favorgen, Vienna, Austria) according to the manufacturer's instructions. RNA sequencing libraries were constructed using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB, #E7760S), incorporating poly(A) mRNA magnetic isolation, according to the manufacturer's instructions.500 ng of total RNA was enriched for polyadenylated transcripts using oligo-dT magnetic beads.