RNA-seq of Rana sylvatica: juvenile dorsal skin
Dorsal skin was dissected in the field and stored in RNAlater (Sigma, cat. no. R0901) at -20 _, then at -80 _ upon receipt at the University of Waterloo. Tissue was removed from RNAlater, flash-frozen in liquid nitrogen, and stored at -80 _ until RNA isolation. Total RNA was isolated using TRI Reagent (Sigma, cat. no. T9424). Enrichment of mRNA and library preparation was performed by The Centre for Applied Genomics (TCAG; The Hospital for Sick Children, Toronto, Ontario, Canada) using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB), followed by paired-end 150 base pair sequencing on one lane of a NovaSeq 6000 S4 flowcell.