18S rDNA of periphyton: NDS experiment tube 135 in Pear Lake, Sequoia, National Park, CA
NDS experiment was deployed in Pear Lake for 21 days; fritted glass disc substrates were collected in the field; DNA was extracted from each disc sample using a modified method of the Qiagen Dneasy PowerSoil Pro kit; samples were shipped to Novogene (USA and China) for PCR amplification, library preparation, sequencing, and bioinformatics clean-up.