HEK293T_DMSO_250uM_Rep3
Total RNA was extracted from HEK293T cells using TRIzol LS Reagent, followed by DNase I treatment and purification. Libraries were prepared using the Illumina TruSeq Stranded Total RNA Kit with rRNA depletion. Sequencing was performed on an Illumina NovaSeq X Plus platform, generating 150-bp paired-end reads.