GSM8634684: RNA_S3848Nr18; Columba livia; RNA-Seq
The performing group upon their return and the control group were sedated and deceased using the cervical dislocation method. The cerebellum was dissected from the brain, secured in RNA later solution and stored at – 20 °t. Tissue was homogenized with the use of zirconium oxide beads (diameter 0.5 mm) in BulletBlender homogenizer (Next Advance Inc., USA). The total RNA was isolated using adjusted method with the use of TriReagent (Ambion, Life Technologies) according to the method described by Chomczyński and PureLink RNA Mini Kit (Invitrogen). The concentration and quality of RNA were determined on TapeStation 2200 using RNA Screen Tape (Agilent Technologies). The samples with RIN (RNA integrity number) ranging from 9.6 – 10.0 (Schroeder et al. 2006), were sent to Library preparation and sequencing by a commercial company (CeGat gGmbH) on NovaSeq 6000 (Ilumina), and finally 100 bp (±50 bp) paired-end reads were generated.