whole genome of high salt-tolerant bacteria
DNA was extracted from a single colony using the Qiagen DNeasy Blood & Tissue Kit (Qiagen, Hamburg, Germany). The DNA was measured for quantity using Qubit and for purity with Nanodrop. Then, 300 ng DNA was taken to start library preparation. The library was prepared using the Illumina DNA Prep, (M) Tagmentation (96 Samples) according to the manufacturers instruction. Then using bridge amplification, each fragment is multiplied into unique, clonal clusters. The templates can be sequenced when cluster generation is finished. Upon the preparation of the library, the sequencing of the sample was carried out in a NextSeq 550 System using a NextSeq 500/550 High Output Kit v2.5 (300 Cycles).