V350158906_L03_28.fq.gz
Small RNA libraries were prepared using the MGIEasy Small RNA Library Prep Kit. Samples were washed to remove residual RNA, barcoded, PCR-amplified (18 cycles), size-selected, and sequenced on the MGI BGISEQ-G400 platform. Quality control and read alignment were performed with Cutadapt, FastQC, Bowtie, and annotation using miRBase and RNAcentral. Expression analysis used DESeq2 and edgeR.