RNA-seq of cerebellum from Eif2b5[R191H] mice
RNA-seq of cerebellum from Eif2b5[R191H] mice
Mice were euthanized with CO2 and tissue was flash frozen in liquid nitrogen after dissection. Tissues were treated with RNA-later ICE (Invitrogen #AM7030) for 24 hr at -20C  RNA extraction was performed following the MaxMAX total RNA isolation kit (Invitrogen #AM1830) RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA)