GSM8943650: Bama pig1, liver, Day 15, 23℃±2℃; Sus scrofa; miRNA-Seq
Extract total RNA from liver tissues of all pigs in the control group and the cold treatment group for 15 days using TRIzol (Life Technologies, Carlsbad, CA, USA) Firstly, total RNA is extracted. Then, RNA molecules within the range of 18-30 nt are enriched and selected by polyacrylamide gel electrophoresis (PAGE). 3' and 5' adapters are ligated to the selected RNA molecules respectively. Subsequently, reverse transcription and PCR amplification are performed on the ligated products. Finally, the bands of approximately 140 bp are recovered and purified by PAGE gel, dissolved in EB solution, and the small RNA library construction and sequencing are completed.