GSM8829065: NOB25_HCT_3; Homo sapiens; RNA-Seq
RNA was harvested usingRNeasy Mini Kit (Qiagen), on-column DNase treatment and purity was quantified. 2ug of protein was submited for RNA seq analysis. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.