GSM8605779: Cortex E15.5 C57BL/6 Kat6b Knock Out, rep 3; Mus musculus; RNA-Seq
Total RNA was collected from cultured NSPCs, E12.5 dorsal telencephalon or E15.5 cortex tissue using a kit (RNeasy mini kit, Qiagen, 74104) according to the manufacturer's instructions and including the optional DNase I digest step. 500 ng to 1 µg total RNA was used to generate sequencing libraries using a kit (TruSeq RNA prep kit v2, Illumina, RS-122-2002) according to the manufacturer's instructions.