AM fungal 18S rRNA gene amplicon sequencing of rhizosphere soil from Stipa breviflora sample SDSB1_3
Total genomic DNA was extracted from rhizosphere soil using the CTAB method. Arbuscular mycorrhizal fungal amplicons were generated using the AM fungal-specific primers AMV4.5NF and AMDGR. Libraries were prepared using the Illumina TruSeq Nano DNA LT Library Prep Kit and sequenced on the Illumina MiSeq platform with paired-end reads. Raw reads were quality filtered, merged, denoised, and checked for chimeras using QIIME2.