GSM9611342: Control, L4; Danio rerio; RNA-Seq
Total RNA was extracted and purified from 20 embryos per group, of 120 hpf using RNeasy® Micro Kit and RNeasy® MinElute® Cleanup kit (Quiagen). One RNA-Seq library was prepared with the embryos pull for each group condition (control, iron and nanoparticle), using Apollo Wafergen 324 system. Quality control was done using TapeStation and qPCR. Finally, the sequencing was performed using four lanes of a NextSeq 500 High-Throughput Sequencer at the Harvard Biopolymers Facility.