GSM8830343: WGBS of 120hr SACs; Homo sapiens; Bisulfite-Seq
Whole-genome bisulfite sequencing was performed to profile DNA methylation. Genomic DNA was extracted, fragmented, and treated with bisulfite to convert unmethylated cytosines to uracil. The converted DNA was then amplified with PCR to add sequencing adapters and generate the final library. Library construction was performed according to the manufacter's instructions.