GSM9140233: 3X-53_PRE; Homo sapiens; RNA-Seq
RNA was extracted by using a Qiagen Mini RNA extraction kit (74004, Qiagen) Total RNAs were polyA enriched, and directional RNA-seq libraries were prepared using the NEBNext Ultra II RNA library prep Kit.The paired-end RNA-seq reads were mapped to human transcripts annotated in GENCODE v44 using Bowtie. Gene level read counts were estimated using rSeq.