GSM9756882: Control 3, biol rep 1; Homo sapiens; RNA-Seq
Total RNA was extracted from all samples using the Rneasy Mini Kit (Qiagen), and residual DNA was removed using the Rnase-Free Dnase Set (Qiagen). mRNA were enriched from 250 ng of total RNA using a PolyA selection. Library preparation was performed using the Illumina® Stranded mRNA Prep ligation kit (Illumina, 20040532), and samples were barcoded with index adapters from the Illumina® RNA UD Indexes (Illumina, 20091655) according to the manufacturer's instructions. Libraries were normalized to 4nM, pooled, and sequenced on a NextSeq 550 Sequencing System (Illumina) in a paired-end (75 bp) mode using a High Output Flowcell v2.5 (Illumina, 20024907).