SC
RNA-seq libraries were prepared from 300 ng of total RNA, using the Illumina Stranded Total RNA Prep with Ribo-Zero Plus (Illumina, San Diego, CA, USA), according to the manufacturers protocol. cDNA libraries were checked on the Bioanalzyer 2100 and quantified by fluorimetry using the Qubit dsDNA Assay Kit (Thermo Fisher Scientific). Sequencing was performed on the Illumina Novaseq 6000 platform, generating 100pb paired-end reads.