GSM8722218: AF22, EFHC1_KD, 1; Homo sapiens; RNA-Seq
RNA was extracted with Sepazol Super G RNA (Nakarai Tesque), followed by Dnase digestion with NucleoSpin RNA column kit. 700 ng of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) following manufacturer's protocols.