DNA-Seq of Chelidonium majus: whole stem genome
Fresh leaves and stems were gathered and immediately frozen in liquid nitrogen, then stored in  80oC. After homogenization of 100 mg Chelidonium majus tissues in mortar and pestle, total DNA was isolated using Qiagen DNA Pro Kit Plant according to manufacturer instructions. Quantity and quality of obtained DNA was measured on the TapeStation System. At least 10g of DNA with highest DNA concentration and DIN above 8 were transferred to sequencing. For short-read sequencing, two Illumina paired-end (PE) libraries were constructed using TrueSeq PCR free kit with an insert size of 550 bp and 350 bp. Library sequencing was performed by an Illumina NovaSeq 6000 system (Illumina, San Diego, CA, USA). To obtain long reads, high molecular weight DNA was size-selected and sequenced using five flow cell R9.4.1 on GridION platform (Oxford Nanopore).