Single-cell RNA-Seq of Mus musculus: BMDM, C57BL/6J, female, 8 weeks
Single-cell 3' RNA-seq libraries were constructed using the SeekOne DD Single Cell 3' library preparation kit. Briefly, single cells were partitioned into droplets with barcoded beads, followed by reverse transcription, cDNA amplification, fragmentation, and adapter ligation. The final libraries were sequenced on the GeneMind SURFSeq 5000 platform with PE154 read length.