Whole-genome resequencing of BC1 populations from Arabidopsis (Col x Ler) x Col crosses (sgo2 +/- and sgo2 -/-)
Whole-genome resequencing of BC1 populations from Arabidopsis (Col x Ler) x Col crosses (sgo2 +/- and sgo2 -/-)
Leaves were removed from plants and stored at -80 C until DNA extraction Greenhouse supplemented with additional light. Plants grown in the greenhouse (16 h day/8 h night) for 3 weeks NucleoMag Plant Kit (Macherey and Nagel, P/O 744400.4) on a robotic device (KingFisher, Thermo) TPase-based DNA library (>95 samples)