Staphylococcus aureus CEO 2 (CRX2109635)
Staphylococcus aureus strain NCTC8325 was cultured under standard conditions and treated with sub-inhibitory concentrations (1/8 MIC) of Amomum tsao-ko essential oil (AEO) or clove essential oil (CEO), with untreated cells serving as the control. Each treatment was performed in three independent biological replicates. Total RNA was extracted using the TRIzol reagent following the manufacturer's protocol. RNA quality and integrity were assessed using an Agilent 2100 Bioanalyzer. rRNA was removed prior to library construction to enrich for mRNA. Sequencing libraries were prepared using the Illumina TruSeq RNA Sample Preparation Kit and sequenced on an Illumina NovaSeq 6000 platform to generate 150 bp paired-end reads.