RNA-Seq of Caenorhabditis latens comma male embryo;PX534
The study profiled single embryos of the closely related species Caenorhabditis remanei (strain PX439) and C. latens (strain PX534), as well as their interspecies hybrids. Embryos were collected at the comma stage, and their sexes were inferred based on the expression levels of the sex-determination genes her-1 and xol-1, with high expression of both genes indicating male embryos. By analyzing the transcriptomes of these species and their hybrids, this study aims to provide insights into gene regulatory divergence, mechanisms of hybrid incompatibility, and the evolution of reproductive strategies within the Caenorhabditis genus.