GSM9078321: S24; Homo sapiens; RNA-Seq
We used the NucleoSpin RNA Mini Kit (Macherey-Nagel, cat. no. 740955.50), following the manufacturer's instructions. RNA concentration was assessed with Qubit 2.0 (Thermo Fisher), a total amount of 400 ng RNA were sequenced. RNA integrity was assessed using Agilent Bioanalyzer 2100, with RNA integrity numbers (RIN) between 9.8 and 10.0. RNA samples were processed by Novogene (Munich, Germany) for library preparation and sequencing, and all samples passed the quality control criteria. Strand-specific libraries were generated based on Novogene´s standard protocol. Samples were sequenced on an Illumina platform to produce 150 bp pairwise reads (PE150) per sample.