GSM9378480: rhAmpSeq, K562, SMARCA4 guide 19, 52 multiplex; Homo sapiens; OTHER
Genomic DNA was extracted by column purification. RhAmpSeq libraries were prepared by the rhAmpSeq CRISPR Library Preparation protocol (IDT), using 50 ng of genomic DNA per sample. Pooled <i>ADSS1</i> libraries were combined with pooled multiplex libraries at a 1 to 4 ratio by mass.