Oxford Nanopore sequencing of Curtobacterium flaccumfaciens pv. flaccumfaciens PD 8682
The sample was processed for Oxford Nanopore Technologies(ONT) sequencing according to the procedure belonging to the Native Barcoding Kit 24 V14. 24 libraries were loaded on 1 PromethION R10.4.1 flowcell and sequenced with the Promethion P2 Solo. The samples were sheared, and a size selection was performed to remove all fragments smaller than 3 kb. The concentration of the fragmented DNA was determined using Qubit 2.0 fluorometer. For quality control, the QIAxcel High resolution Genomic DNA was used. The sample was normalized to an input of 400 ng. Library preparation was performed using the Native Barcoding Kit 24 v14, following the gDNA protocol. This protocol included DNA repair and end-prep, followed by barcode ligation. After these steps, the sample was measured again with Qubit and pooled into a single pool with 23 other samples (not part of this project). Adapter ligation was then performed. Finally, the flowcell was prepared, and the pooled samples were loaded onto the flowcell. Sequencing of the samples was performed on one promethion flowcell on the PromethION P2 Solo sequencer. The samples were basecalled using MinKNOW 24.11.8 using the HAC model.