OsDOF3_CUTTAG (CRX2175582)
The homogenate was filtered through a 40-um cell strainer, and nuclei were stained with 1 ug/mL DAPI (4',6-diamidino-2-phenylindole) for fluorescence-activated nuclei sorting. Flow cytometric analysis and sorting were performed using a Sony SH800 Cell Sorter equipped with a 405-nm laser. A minimum of 100,000 nuclei were collected per biological replicate. The sorted nuclei were immediately processed for chromatin enrichment and library preparation steps using the CUT&Tag Assay Kit (Vazyme, TD904-C2) according to the manufacturer's instructions.