GSM8462717: Leaf, Cool, 475, rep 1; Wahlenbergia ceracea; RNA-Seq
Total RNA was extracted from snap-frozen and ground leaf tissue using the Spectrum Plant Total RNA Kit (Merck, New Jersey, USA) with on-column DNase I digestion. The construction of cDNA libraries was conducted with 250 ng RNA input using the Illumina stranded mRNA prep ligation protocol, as per manufacturer's instructions except that reaction volumes were scaled by one-half and 12 PCR cycles were used for library amplification.