GSM8862385: Control_Male_H3K79me2_R3; Mus musculus; ChIP-Seq
Cortical tissue was homogenized with a douncer in homogenization buffer supplemented with 1 mM DTT, 0.15 mM spermine, 0.5 mM spermidine, RNasin ® Plus Ribonuclease Inhibitor (Promega N2611), and EDTA-free protease inhibitor (Roche). Nuclei were isolated using an iodixanol density gradient and ultradentrifugation. TruSeq ChIP Library Preparation Kit