WC1 (CRX2065396; CRA031353)
Total RNA was isolated from six frozen ovarian tissue samples with Trizol reagent (Takara, Dalian, China), following the manufacturer's protocol. We assessed RNA quantity and integrity through dual approaches: fluorometric quantification with the Equalbit RNA BR Assay Kit on a Qubit1 4.0 Fluorometer (Invitrogen, CA, USA) and fragment size analysis via capillary electrophoresis on a Qsep-1 system (Bioptic Inc., Taiwan, China). Starting from 3 microg of total RNA per sample, sequencing libraries were prepared with the VAHTS Universal V6 RNA-seq Library Prep Kit.