Human sample from Homo sapiens
High molecular weight (HMW) DNA was extracted from either 3 mL of freshly collected blood or 10-15 million cultured cells using the Qiagen Puregene extraction protocol as modified by ONT for DNA extraction from cell lines version 3.  10  g of HMW DNA was size selected with the ONT Short Fragment Eliminator Expansion kit EXP-SFE001 protocol version 1. Libraries were prepared for sequencing with ONT Ligation Sequencing Kit V14 SQK-LSK114 kit, following the Ligation Sequencing gDNA V14 - human sample on PromethION procedure.  Either five MinION SpotON R10.4.1 flow cells or one PromethION R10.4.1 flow cell were used to sequence each sample. 500 ng of SFE library was loaded onto each flow cell following ONT procedures with reagents from SQK-LSK114 and EXP-FLP002 kits. Adaptive sampling sequencing runs were set for 72 hours with a minimum read length of 1000 bp and FAST live basecalling. Adaptive sampling was enabled and a reference file containing MHC and KIR haplotype sequences was submitted to the control software. Libraries were reloaded every 24 hours until a full run time of 72 hours was reached. After sequencing was completed, ONT reads were rebasecalled using Dorado (v.0.5.3) and the super accurate (SUP) model: version 4.1 for sequencing runs at 4 kHz or version 4.3 for sequencing runs at 5 kHz. Fastq sequences were generated for reads with a minimum quality score of Q10.