WGS of Poecilia latipinna
We flash-froze in liquid nitrogen and stored at 80 C until ready for isolation. High molecular weight (HMW) DNA extraction was performed using the 10x Genomics Demonstrated Protocol: DNA Extraction from Single Insects (10x Genomics, Pleasanton, CA, USA) and then purified and concentrated through standard ethanol precipitation. The final DNA quantity was determined using the high-sensitivity Invitrogen Qubit Fluorometer kit (Invitrogen). Final DNA quality was evaluated on a 0.7% agarose gel and visualized using the Uvitec Cambridge Uvidoc HD6 UV Fluorescence and Colorimetry instrument. All isolated DNA was shipped to HudsonAlpha (Huntsville, AL, USA) for library construction and long-read sequencing on a PacBio Sequel II in CCS/HiFi mode.