RNA-seq of SPI1-knockdown THP-1-derived macrophages treated with ox-LDL, biological replicate 3
THP-1 cells carrying an SPI1-targeting shRNA were differentiated with PMA for 24 h, allowed to recover for 24 h, and treated with 50 ug/mL ox-LDL for 48 h. Total RNA was extracted using TRIzol reagent. RNA-seq libraries were constructed using the VAHTS Universal V10 RNA-seq Library Prep Kit (Premixed Version) according to the manufacturer's instructions. Biological replicate 3.