GSM9314751: ACLY WT, low glucose, rep3; Mus musculus; RNA-Seq
RNA was purified using the Qiagen RNeasy Plus Mini kit (74134) according to the manufacturer's instructions and eluted in 50mL volume. To improve RNA purity, 125mL ethanol and 5mL 3M sodium acetate was added, and RNA was re-precipitated at -20°C for at least 1 hour. After centrifuging for 10 min at 12,000g, the RNA pellet was washed with 75% ethanol before resuspending in nucleus-free water. RNA was incubated for 5-10 min at 56°C to increase solubility and stored at -80°C. Illumina library prep, sequencing, and QC was performed by Novogene.