GSM8462269: Jag mutant 2; Mus musculus; RNA-Seq
Postnatal day (P)15 mouse livers were dissociated from two animals of each genotype, wild-type and Jag1+/−, using a cold protease protocol to avoid thermal and long collagenase incubation times that may induce technical changes to the transcriptional signatures (dx.doi.org/10.17504/protocols.io.bhpcj5iw). Given the small sample size, only left lobes from females were used to reduce variability. Single cell RNA Sequencing was performed using 10x Genomics 3' version 3.1 chemistry, targeting a minimum of 30,000 UMI per cell barcode (on average) and 7k cell barcodes per sample Dual-indexed transcriptome libraries were pooled and sequenced across multiple Illumina Novaseq 6000 S4 or X plus flow cells (Illumina) with PE150+10+10 or PE100+10+10 settings.