Illumina sequencing of soil fungi, sample FL22
DNA was extracted from a 5 g subsample of soil sample of each plot using the PowerMax Soil DNA Isolation Kit (MoBio Laboratories, Inc.). Identification of the soil fungal community composition, the target marker region of the ribosomal ITS2 was amplified with degenerate primer pair fITS7:fITS7o and ITS4. The ribosomal ITS2 region were sequenced by Novogene Company (United Kingdom) using 2  250 bp paired-read sequencing approach on an Illumina NovaSeq platform.