RNA-Seq ofH-2
Extract relevant cellular RNA and measure RNA concentration using Nanodrop. Subsequently, mRNA was enriched using magnetic beads with Oligo (dT). Then, a cDNA library was constructed by reverse transcription and PCR amplification. After passing the quality inspection of the library, pooling was performed on the library, and finally Illumina Novaseq 6000, PE 150 sequencing technology was used to perform on machine sequencing of RNA.