GSM8722401: T-PLL patient sample P11, 8h, control + IL6; Homo sapiens; RNA-Seq
RNA was isolated using the Qiagen RNeasy Plus Mini Kit (#74134) according to the manufacturer's instructions. RNA quality and concentration were assessed using the 4150 TapeStation (Agilent, Santa Clara, USA). Library preparation was conducted with the Illumina TruSeq Stranded Total RNA Library Prep Gold Kit (rRNA-depleted total mRNA, #20020599). The samples were processed without ERCC RNA Spike-In controls. Paired-end sequencing (2x100bp) was performed with 50 million reads per sample.