RNA-Seq of Gossypium anomalum leaf
Total RNA was extracted from leaf tissues of diploid cotton species (Gossypium anomalum, Gossypium sturtianum, and Gossypium rotundifolium) using a standard TRIzol-based protocol. RNA quality was assessed using a Bioanalyzer. RNA-seq libraries were constructed using the Illumina TruSeq Stranded mRNA Library Prep Kit, following the manufacturers instructions. Libraries were sequenced on an Illumina NovaSeq 6000 platform to generate paired-end 150 bp reads.