sequencea of bacteria
The bacterial project uses the highly variable V3V4 region of the bacterial 16S rRNA gene for sequencing; PCR amplification uses specific primers for the V3-V4 region of the bacterial 16S rRNA gene: 338F (5-barcode+ACTCCTACGGGAGGCAGCA-3) and 806R (5-GGACTACHVGGGTWTCTAAT-3).