ABA24h_2 (CRX2109142)
Libraries were generated using Ad1_noMX and Ad2.1-2.4, then amplified for 7-9 total cycles. After amplification, libraries were purified with AMPure beads (Agencourt) to remove primer dimers. Quality was assessed on an Agilent Bioanalyzer with the High-Sensitivity DNA kit, and concentration was measured using Qubit. All libraries were sequenced on an Illumina HiSeq 2500 (150 bp paired-end) and subsequently on an Illumina NextSeq 500 (40 bp paired-end). Illumina adapters were trimmed with Trimmomatic, and the first 10 bp of the 50 bp reads were removed with fastx_trimmer. Reads from both runs were pooled and aligned to the reference genome using Bowtie6 with default parameters. This processing allows direct comparison with published ChIP-seq and FAIRE-seq data sets.