RNA-seq of developmentally- and adult-labeled Tlx3-Cre-positive neurons in the mouse primary visual cortex
RNA-seq of developmentally- and adult-labeled Tlx3-Cre-positive neurons in the mouse primary visual cortex
A portion of the mouse primary visual cortex was microdissected and a single cell suspension was created using progressively smaller fire-polished Pasteour pipettes. Cells were FACS sorted for fluorescence. RNA for bulk RNA-sequencing was purified with the Single Cell RNA Purification Kit (Norgen), and columns were treated with a Dnase to remove genomic DNA (Norgen). The isolation was performed according to the manufacturerâ€™s instructions mRNA-seq libraries were obtained using a SmartSeq2 approach using purified RNA as input.