OsVAL2_control_ChIP (CRX2175585)
Sheared chromatin was incubated with an antibody against FLAG overnight. Immune complexes were captured with Protein A magnetic beads, washed, and eluted. After reversing crosslinks, the immunoprecipitated DNA was purified. Sequencing libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit for Illumina with 10 ng of input DNA or ChIP-enriched DNA, following the manufacturer's protocol.