direct RNA ONT sequencing of P. falciparum Dd2 Gametocytes stage V in vitro polyadenylated
Libraries were prepared from 150 ng RNA, which was in vitro polyadenylated E. coli Poly(A) Polymerase (New England Biolabs) for 75 sec at 37C, using the SQK-RNA002 kit and sequenced on a MinION flow cell (R9.4.1). Only reads above 200 nt were sequenced. Fastq files were generated using guppy-6.4.2