Bovine viral diarrhea virus 1 isolate GS2025-1
The Illumina platform's next-generation sequencing method involves taking the genomic DNA to be sequenced as a sample, breaking it into short fragments, attaching sequencing adapters to both ends, immobilizing the fragments on a chip, amplifying them via bridge PCR to form DNA clusters, then using fluorescently labeled dNTPs for DNA synthesis. Specific fluorescent signals emitted by different bases are detected by lasers and converted into base sequences, thereby obtaining sequencing results for a large number of fragments.