GSM8895960: K562, Day 0, Rep 1; Homo sapiens; OTHER
DNA was isolated from sorted cells using GeneJET™ Genomic DNA Purification Kit (ThermoFisher Scientific) according to the manufacturer's protocol. Exogenous NAT10 from isolated genomic DNA was amplified by PCR and ran on 1% agarose gel. The fragment of interest was gel extracted by GeneJET Gel Extraction Kit (ThermoFisher Scientific) following the manufacturer's protocol. Amplicons were end-prepped using NEBNext® Ultra™ II End Repair/dA-Tailing Module (New England Biolabs).  The end-prepped DNA was native barcode ligated using Native Barcoding Kit 24 V14 (Oxford Nanopore, SQK-NBD114.24) using Blunt/TA Ligase Master Mix (New England Biolabs) followed by purification with 0.4-fold AMPure XP Bead-Based Reagent (Beckman Coulter). The native barcoded samples were pooled and ligated to a native adaptor with Quick T4 DNA Ligase (New England Biolabs), and then the pooled ligated sample was cleaned with 0.4-fold AMPure XP Bead-Based Reagent (Beckman Coulter). The ligated pool was quantified using Qubit™ dsDNA Quantification Assay Kits (ThermoFisher Scientific). DNA pool of 12 µl was added to 37.5 µl sequencing buffer (SB) and 25.5 µl library solution (LIS), making a final volume of 75 µl.