GSM8830130: Sham_heart_2; Mus musculus; RNA-Seq
Hearts were digested and cardiac endothelial cells sorted by FACS to generate cells for inclusion in scRNAseq experiment The 10X Genomics Chromium Next Gem Single Cell 3' library kit v.3.1 was used to prepare barcoded mRNA following the guidelines from the supplier. Single Index kit T Set A was used to label each reaction. Samples were loaded onto 3 separate Illumina kit High-throughput 2x75 (<400 million reads) flow-cells and sequenced using Illumina Nextseq, using the settings of: Read1: 28 cycles; Read2: 8 cycles; Read3 (i7): 114 cycles