TCR Amplicon of human: healthy donor CD3+ T cells
Single cell TIRTL-seq: Healthy donor PBMCs derived CD3+ T cells directly sorted one cell per well in a 384 well plate, lysed and mRNA reverse transcribed using poly-A and random hexamers. cDNA amplified with TCR V segment and C segment multiplex primers in PCR1 which was then amplified with well specific dual-index primers containing Illumina adapters in PCR2. Libraries made using unique pairs of TCR-alpha and TCR-beta C-segment primers with inline barcodes (human abTCR-ID10) and were pooled for sequencing with library from another single cell experiment. This is demultiplexed data for ID10 for wells that contained cells.