Raw reads: Elk-3 (ecotype_ID: 22125)
DNA was extracted using NucleoBond HMW DNA kit (Macherey-Nagel, Düren, Germany), as per the manufacturer's instructins. Library preparation followed the Nanopore SQK-LSK114 protocol and kit with the following modifications; approximately 1.2-1.5 µg of size-selected DNA in a volume of 48 µl was used for library preparation, the first bead clean-up after nic-repair and end-prep were replaced by Short Read Eliminator (SRE) size selection (PacBio), and libraries were sequenced in a R10.4.1 Promethion flowcell, with base-calling and DNA methylation quantification performed using dorado (v0.8.3.) with model dna_r10.4.1_e8.2_400bps_sup@v5.0.0 and --modified-bases 5mC_5hmC. Filtlong (https://github.com/rrwick/Filtlong) was used to retain reads >30 kb.