GSM9429709: 3-CM2; Mus musculus; RNA-Seq
The total RNA was extracted using TransZol Up Plus RNA Kit (Cat#ER501–01, Trans) according to the manufacturer's instruction, and RNA integrity was checked using an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US). Total RNA was qualified and purified using RNA Clean XP Kit (Cat A63987, Beckman  Coulter, Inc., CA, USA) and RNase-Free DNase Set (Cat#79254, QIA_x0002_GEN, GmBH, Germany). The sample library was constructed from the purified total RNA through a series of steps, including mRNA separation, fragmentation, first-strand cDNA synthesis, second-strand cDNA synthesis, end repair, 3′ end addition, ligation, and enrichment. The concentration and size of the sample library were determined using Qubit® 2.0 Fluorometer and Agilent 2100, respectively. The sequencing process was run and monitored using Illumina HiSeq 2500 System, and real-time data analysis was performed using HiSep Control software.