RNA-seq of Staphylococcus aureus USA300 RS14325 knockout mutant
Once the total RNA samples had passed quality control, rRNA was removed using a kit. The library was constructed using the Truseq RNA Sample Prep Kit; however, during the synthesis of the second strand of cDNA, dUTP was substituted for dTTP in the dNTPs, ensuring that the second strand contained the bases A, U, C and G. Prior to PCR amplification, the second strand of cDNA was digested using UNG enzyme, thereby ensuring that the library contained only the first strand of cDNA.