GSM8723228: Rt88_low nitrogen_acetate_88h_biol rep 3; Rhodotorula toruloides; RNA-Seq
Beaten with 0.5 mL of glass beads, acid-washed 425-600 um (30-40 U.S. Sieve) in a 1.5 mL RNAse-free tube using Qiagen RNEasy kit on a Tissue-Lyser II at 30 Hz for 60 s three times.  Turbo DNAse treatment for 30 minutes followed by a second RNEasy kit purification step. RNA-Seq libraries were prepared with the Stranded mRNA library kit (Watchmaker). The libraries were pooled; quantitated by qPCR and sequenced on one 10B lane for 151 cycles from both ends of the fragments on a NovaSeq X Plus with V1.0 sequencing kits (Illumina). Fastq files were generated and demultiplexed with the bcl2fastq v2.20 Conversion Software (Illumina).