GSM8510815: E20 CD45 negative scrnaseq; Gallus gallus; RNA-Seq
Lungs were extracted from embryos and subsequently dissociated. They were stained for the major cell populations of the lungs and FACs sorted. Roughly equal proportions of collected cell populations were mixed for scMultiome. Only epithelial cells or samples depleted of immune cells were taken for scRNA. Sorted cells were then sequenced. 50 cycles for read 1, 49 cycles for read2, 8 cycles for index1, and 24 cycles for index2 28 cycles read1, 90 cycles read2, 10 cycles Index1, and 10 cycles Index2