Single-cell RNA-seq analysis of patient derived organoids from colorectal cancers
Single-cell RNA-seq analysis of patient derived organoids from colorectal cancers
Organoids were colected and signle cells were prepared in TrypLE. 10x Genomics Chromium was used for single cell capture and cDNA preparations according to the Single Cell 3' v3 Protocol recommended by the manufacturer. 10x Genomics Chromium was used for single cell capture and cDNA preparations according to the Single Cell 3' v3 Protocol recommended by the manufacturer.