GSM8605935: Low-CDC-sensitive-2; Homo sapiens; RNA-Seq
Total RNA was isolated using the RNeasy micro – or mini kit (Qiagen) from cell pellets of the U2932 cell lines or of the FACS-isolated B-cells (CD19+CD3-) from DLBCL patient samples. RNA quality and quantity was assessed using the High Sensitivity RNA ScreenTape Analysis (Agilent), and RNA integrity numbers (RIN) of >8.5 were obtained for all samples. A KAPA mRNA hyperPrep kit was used for mRNA library construction. The libraries were sequenced with paired-end reads on a NovaSeq-6000. Bulk RNA-seq