Lettuce cv. Salinas ONT exp2
HMW DNA was extracted from young seedlings using KeyGenes proprietary HMW protocol. Size selection was performed on the genomic DNA using the PacBio SRE XL kit. The SQK-LSK114 library prep kit was used to construct a 1D library. The sample was sequenced on a PromethION P24. Basecalling was performed in real-time by the PromethION compute module (v22.10.5) using MinKNOW 5.3.1.