Whole genome sequencing of pooled HIV-1 clinical sample using Oxford Nanopore PromethION
Viral RNA extracted from a pooled plasma sample consisting of 10 HIV-1 infected individuals was reverse transcribed into cDNA. Whole-genome amplification was performed using overlapping multiplex PCR primers. Libraries were prepared using the Oxford Nanopore Technologies Native Barcoding Kit (SQK-NBD114-24) and sequenced on the PromethION platform. The resulting sequencing reads represent a pooled HIV-1 sample and were used for downstream genome assembly and consensus sequence generation.