GSM8996464: Demyelination Control id105; Homo sapiens; RNA-Seq
brain organoids with and without microglia were lysed using MagNA Pure DNA Tissue Lysis Buffer  and RNA isolated using the MagNA Pure 96 System (Roche). 10ng of high quality total RNAs were used to prepare sequencing libraries using the Illumina Stranded 28 Total RNA Prep with Ribo-Zero Library Prep Kit (Illumina) according to the manufacturer's instructions. All libraries were multiplexed into a single pool and sequenced on an Illumina NovaSeq 6000 instrument using an SP flowcell. Raw sequencing data were demultiplexed using the BLC Convert demultiplexing pipeline and each library generated approximately 28 million paired-end 50 bp reads.