Anti-CD45RC antibody targets CD45RChigh T and B cells to mitigate pathogenic immune responses
Anti-CD45RC antibody targets CD45RChigh T and B cells to mitigate pathogenic immune responses
Freshly isolated PBMCs of three healthy donors were labelled with anti-human Hashtag antibodies TotalSeq-C (Biolegend, San Diego, CA) and were pooled together Disintegration of cells or tissues (cell lysis), inactivation of intracellular nucleases, and separation of nucleic acids from the other cell components Single cell libraries were performed according to Chromium Next GEM Single Cell 5' Reagent Kits v2 protocol (10X genomics, San Francisco, CA). For each sample, cells were processed in one of eight wells and multiplexed using antibody hashtags