GSM8463348: Pancreas, KC WT, Mouse 4; Mus musculus; RNA-Seq
RNA extraction was performed as described in (Smith et al., 2022). Frozen pancreas was pulverised while covered in liquid nitrogen, transfer to TRIzol and homogenised. RNA was isolated with the phenol/chloroform extraction method and purified using the Qiagen RNeasy kit. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.