S45 (CRX1279102)
Intestinal bacterial DNA was extracted by using a HiPure Stool DNA Kit (Magen Biotech Inc., Guangzhou, China), then the V3-V4 region of the bacterial 16S rRNA gene was amplified using the primers: 338F and 806R. PCR products for each sample were pooled and purified using a PCR fragment purification kit. The amplicon products were sequenced using an Illumina MiSeq platform.