GSM8895887: Arabidopsis Col-0 leaf, CMV-1; Cucumber mosaic virus; RIP-Seq
Total RNA was isolated and purified using TRIzol reagent, and Poly (A) RNA is purified from 50 µg total RNA using Dynabeads Oligo (dT) using two rounds of purification. The poly(A) RNA was reverse transcribed with adaptors. The reverse transcription product was purified by magnetic beads and connected to the purified product using the sequencing adapter in the SQK-RNA002 kit. The constructed cDNA library was accurately quantified using Qubit. After the library was constructed, a certain amount of cDNA library was added to the Flow Cell and transferred to the Oxford Nanopore PromethION sequencer for real-time single-molecule sequencing.