GSM8829640: High-IMF pectoral tissues M0011; Anser cygnoides; RNA-Seq
Total RNA was extracted from breast muscle tissues employing the TRIzol reagent (Invitrogen).(1) NanoDrop 2000 spectrophotometer: detection of RNA purity and concentration; (2) Agient2100/LabChip GX detection: accurate detection of RNA integrity. (1) Enrichment of eukaryotic mRNA with magnetic beads with Oligo (dT); (2) mRNA was randomly interrupted by Fragmentation Buffer. (3) Using mRNA as template, the first cDNA chain and two strands were synthesized, and the cDNA was purified; (4) The purified double-stranded cDNA was then end-repaired, A-tail was added and sequencing joints were connected, and AMPure XP beads were used for fragment size selection; (5) cDNA library was obtained by PCR enrichment