RNA-Seq of Cryptococcus gattii
Total RNAs were isolated by the Trizol reagent (Invitrogen, Carlsbad, CA) and RNAeasy mini kit (Qiagen). RNAs were treated with Turbo RNase-free DNase (Ambion) to remove genomic DNA. polyA-RNA was purified from total RNA, processed, and paired-end directional libraries (150 bp) were prepared and sequenced using an Illumina HiSeq 4000 platform (Novogene, Davis, CA).