Archaeal 16S metagenom of groundwater from well 9 (Kultuk reservoir)
The V5-V6 variable region of the archaeal 16S rRNA gene was amplified by PCR with primers, containing rRNA-specific parts: Arch-0787F (5-ATTAGATACCCSBGTAGTCC-3) and Arch-1059R (5-GCCATGCACCACCWCCTCT-3). The libraries were analyzed using Illumina MiSeq Standard Kit v.3 (Illumina) in the Shared Access Center "Genomic technologies, proteomics, and cell biology" of the All-Russia Research Institute for Agricultural Microbiology (Pushkin).