RNAseq of Mus musculus: adult retinae
Adult female (postnatal [pn] Day 40) WT and Vlgr1/del7TM mice were euthanized by cervical dislocation. Eyeballs were dissected from the mouse skulls and retinae were extracted from the eyes. Tissues were flashfrozen using liquid nitrogen. Tissues were homogenized in RNeasy lysis buffer using 27 gauge needles, following RNA was isolated according to the instructions of the Qiagen RNeasy Mini Kit. RNA quality was determined using a Nanodrop (Thermo Fisher Scientific), and RNA was subsequently stored at -80C. Whole transcriptome sequencing was performed by the company Novogene. mRNA sequencing was performed using the Illumina platform. Pair end reads were mapped and quantified. Following, differential gene expression analysis and GO enrichment analysis were performed.