RNA-Seq of WT and Mecp2 KO cerebellum after transplantation with Neural/Precursor Cells (NPCs)
RNA-Seq of WT and Mecp2 KO cerebellum after transplantation with Neural/Precursor Cells (NPCs)
cerebellum dissection from experimental animals Transplantation of neural precursor Cells (NPCs, 10 million of cells) or PSB (10ul) intra-cisterna magna, under anaesthesia RNA was extracted by using Purezol (cod.7326890; Bio-rad) following . DNasi I Amplification Grade (cod.AMPD1; Sigma-Aldrich) was used to remove eventual genomic DNA contamination and a second RNA extraction was performed in Purezol. RNA integrity was verified using the RNA 6000 Nano Kit on a Bioanalyzer (Agilent Technologies); all samples had a RIN>9. RNA was quantified using Qubit RNA BR Assay Kit. Libraries were prepared accordingly to the following guides: https://emea.support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/illumina_prep/RNA/illumina-stranded-mrna-reference-guide-1000000124518-02.pdf