Target enrichment and raw reads of Systaria hainanensis using UCE RTA_v3 probes
Genomic DNA was extracted using the DNeasy Blood & Tissue Kit (QIAGEN) with RNase A treatment. DNA quality was assessed by Qubit and gel electrophoresis, then fragmented to 300600 bp. Dual-indexed libraries were prepared using the NGS Fast DNA Library Prep Set for Illumina (CWBIO), pooled (1524 samples per pool), and enriched for UCEs using the myBaits protocol 5.01 with the RTA_v3 probe set (3818 UCE loci; Zhang et al. 2023). Enriched libraries were sequenced on Illumina NovaSeq or DNBSEQ-T7 platforms (150 bp paired-end).