GSM8511751: N2, L1, rep2; Caenorhabditis elegans; RNA-Seq
L1 larvae were resuspended in TRIzol and underwent repeated freeze-thaw cycles. Total RNA was extracted and purified using the Qiagen Rneasy kit with on-column DNAse digestion. NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (catalog number E7760L), and NEBNext Multiplex Oligos for Illumina Unique dual (catalog number E6448S) were used for library prep.The mRNA was fragmented and copied into first strand cDNA using reverse transcriptase and random primers. The 3' ends of the cDNA were then adenylated and adapters were ligated. The products were purified and enriched by PCR to create the final cDNA library.