GSM8722199: cachectic_Male_17; Mus musculus; RNA-Seq
Total RNA was extracted from liver using TRIzol (Thermo Fisher), followed up by a clean-up step using RNeasy kit (Qiagen). One microgram of total RNA of each sample was submitted to the WCM Genomics Resources Core Facility. Following isolation, RNA integrity was checked using an Agilent Technologies 2100 Bioanalyzer and used if RNA Integrity Number (RIN) value was greater than 8. The library construction was done following the protocol from Illumina TruSeq Stranded mRNA Sample Preparation kit. DNA fragments were sequenced using HiSeq4000 on SR100 format