GSM9610961: Skin, blank cryoMN, biol rep 1; Mus musculus; RNA-Seq
Total RNA was extracted from three biological replicates of the NC, B-CryoMN, and H-CryoMN groups under identical experimental conditions. The concentration and purity of the isolated RNA were determined using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, USA). Subsequently, RNA sequencing libraries were constructed using the Illumina Stranded mRNA Prep Ligation Kit (Illumina, San Diego, CA, USA) and sequenced on the Illumina platform.