GSM8513219: Treatment time 2; Saccharomyces cerevisiae; OTHER
Samples were taken at 0, 1, 2, 3, and 4 days. DNA was isolated with a Thermo Scientific Pierce Yeast DNA Extraction Reagent Kit, and the component from the library was isolated by PCR with the Invitrogen AccuPrime SuperMix I kit. Designed sequences were provided as an Excel file to GenScript. The sequence pool was synthesized by massive parallel synthesis, stripped from the chip, amplified by PCR appending biorep barcodes, cloned into parental vector with estimation of library complexity by manual colony counting. DNA sequencing was conducted by NovoGene.