RNAseq of iTAM and mTAMs isolated from tumors in Junf/f and Jun Csf1r KO mice
RNAseq of iTAM and mTAMs isolated from tumors in Junf/f and Jun Csf1r KO mice
300mg tumour fragments digested with tumour dissociation kit (Miltenyi BioTec) were stained with CD45.2-PE and CD45+ cell isolated with anti-PE beads (StemCell) after enrichment iTAM and mTAM populations were sorted by flow cytometry (10000 cells). Nucleic acid extraction protocol: Arcturus PicoPure. Sample prep (RNA-cDNA) \"Smart-Seq2\" - adapted from Picelli et al 2013 https://www.nature.com/articles/nmeth.2639, in-house method on robotics. The libraries for RNA-seq were prepared using ILL DNA Library Prep Tagmentation LV (Pur,FA) and quality of the RNA libraries was confirmed using fragment analyser