=CONCATENATE("GBS of ", A53, ": auldt leaf")
We used the DNeasy Plant Mini Kit (Qiagen, Valencia, CA, USA) to extract and purify DNA from leaf tissue, according to the manufacturers instructions. We determined genomic integrity on 1% agarose gels and quantified DNA concentration using a Qubit Fluorometer (Thermo Fisher Scientific). Genotyping by sequencing (GBS) libraries were prepared by Ecogenics GmbH (Switzerland) according to Elshire et al. (2011) and using the restriction enzymes EcoRI and MseI. The resulting PCR products were sequenced on an Illumina NovaSeq platform using an S2 flow cell (2100 bp).