Soil sample M_G1 from greenhouse experiment, Spain, mycorrhizal soil, grass mixture
DNA was extracted from 0,5 g of frozen soil with the DNeasy PowerSoil Pro Kit (Qiagen) following the companys instructions. AM fungal sequences were amplified from soil DNA extracts using the fungal-specific primers for the second internal transcribed spacer (ITS2) ribosomal RNA gene region: forward primer - fITS7 (5' GTGARTCATCGAATCTTTG 3') (Ihrmark et al., 2012); reverse primer - ITS4 (5' TCCTCCGCTTATTGATATGC 3') (White et al., 1990). These primers also included the Illumina sequencing primer sequences attached to their 5' ends.