E13.5 LK WT_ATAC_Rep2
ATAC-seq libraries were constructed using Hyperactive ATAC-Seq Library Prep Kit for Illumina (Vazyme #TD711) and TruePrep Index Kit V2 for Illumina (Vazyme #TD202) according to product manual. 65000 cells were used per library with 13 cycle of PCR amplification. Single size exclusion was performed to remove small fragments with beads provided in the kit. Libraries were validated by Agilent high sensitivity dna kit.