GSM9139506: NGO treated nonGVHD patient's PBMC 5, CD3/28, IL-2 activated, 48h; Homo sapiens; RNA-Seq
Total RNA was extracted and purified from hPBMCs studied in vitro using the TRIzol reagent. 100ng of total RNA was used to construct sequencing library Libraries were generated using the SMARTer Stranded RNA-Seq Kit (Takara Bio USA, Mountain View, CA, USA) according to the manufacturer's protocol.