GSM8513109: UM-UC-3 cells, shCDH11#2, 3; Homo sapiens; RNA-Seq
Ctrl and CDH11 KD cell lines were precultured in 12-well plate (2 × 105 cells/well) for 24 hours. Total RNA samples were extracted using RNeasy spin column kits and DNase I. RNA integrity number was verified using an Agilent 2100 Bioanalyzer.RNA-seq was performed on an Illumina NextSeq 500 with a read configuration of 75 bp for single reads; 3 million reads were generated per sample.