GSM8655602: Knee OA synovium_infla_2; Homo sapiens; RNA-Seq
Synovial tissues  were collected surgically. Immediately after collection, they were removed from bone and adipose tissues using surgical scissors, and cryopreserved by CryoStor CS10 (HemaCare Corporation) at -80℃. Cryopreserved synovial tissues were enzymatically treated and resuspended at a concentration of 1,000 cells/µL. Cell viability, confirmed at approximately 80%, was assessed using the trypan blue exclusion method with automated counting by LUNA-FL (Logos Biosystems). The cell suspension was prepared according to the protocol of Chromium Next GEM Single Cell 3ʹ Reagent Kits (v3.1 10X Genomics), and a single cell emulsion was produced using Chromium Controller (10X Genomics).