16Smet metabarcoding of 10L filtered shipboard freshwater from Niskin Sedna: freshwater control
10L of water shipboard freshwater added to bleached Niskin Sedna was filtered through a 0.8 micron prefilter. DNA was extracted using the Qiagen PowerSterivex kit. Primers targeting the 16S mitochondrial gene (Kelly et al. 2016) were used to generate amplicons sequenced on an Illumina MiSeq with v3 chemistry generating paired-end reads. Samples were dual indexed with Nextera indices following the Illumina 16S metagenomics protocol (Illumina Part# 15044223 Rev B).