GSM8635073: balixafortide/anti-PD-1 tumor 2; Mus musculus; RNA-Seq
Tumors removed from mice; digested with collagenase/DNAse I in Miltenyi OctoDissociator; following lysis of red blood cells, isolation of CD45+ cells with immunomagnetic beads; recovered cells used at a ratio of 3:2 CD45+ to CD45- cells for DropSeq single cell RNA sequencing bar coded single cells with polyA enrichment for RNA for sequencing