GSM9892950: CTRL1, HCAEC cell, vehicle for 20 hours; Homo sapiens; RNA-Seq
Aurum Total RNA kit according to instructions Strand-specific mRNA libraries were constructed using a dUTP-based protocol. Briefly, poly(A)+ mRNA was enriched using oligo(dT)-conjugated magnetic beads, fragmented, and reverse-transcribed to generate first-strand cDNA. Second-strand cDNA synthesis was performed with dUTP incorporation to preserve strand information. Following end repair, adapter ligation, and PCR amplification, libraries were converted into DNA nanoballs (DNBs)