High-depth ONT RNA-seq of NmB M0579 modD1::kan KO: replicate KO4
Total RNA was extracted (TRIzol) from Neisseria meningitidis serogroup B strain M0579 harvested at mid-log phase after aerobic growth in GC broth supplemented with 30 M desferrioxamine (iron-deplete conditions) at 37C with 200 rpm shaking. Libraries were prepared using a standard Oxford Nanopore barcoded RNA-seq workflow at a collaborating facility and sequenced on an Oxford Nanopore platform. Demultiplexed reads were exported as gzipped FASTQ files for each biological replicate.