Single-cell transcriptome sequencing of A6 (CRX865777)
Three sets of human D56 forebrain organoids derived from three sets of CTRL and WS iPSCs were dissociated with 3 mL Tryp-LE (Sigma-Aldrich) and for 15 minutes at 37 degreeCC, followed by 2 washes with 3% FBS in DPBS and gentle titration using a P1000 pipette.