GSM9378395: AP_poly; Homo sapiens; RNA-Seq
Trizol-LS(for RNA extraction from liquid) The library construction protocol in the file is a standard Illumina-compatible method involving mRNA purification from total RNA using poly-T magnetic beads, fragmentation with divalent cations, random hexamer-primed first-strand cDNA synthesis followed by RNaseH degradation and second-strand synthesis, end blunting and adenylation, hairpin adaptor ligation, size selection of 370-420 bp fragments with AMPure XP beads, PCR amplification, and quality assessment via Qubit fluorometry, Agilent 2100 insert size detection, and qRT-PCR effective concentration quantification.