Target enrichment and raw reads of Orcevia timburtoni using UCE RTA_v3 probes
UCE libraries were prepared following: The genomic DNA extracted from each specimen were first fragmentated by sonication (target size of approximately 300600 bp). The fragmented DNA (21 ul) were then used as input for DNA library preparation with the NEXTFLEX Rapid DNA-Seq Kit 2.0 (Bioo Scientific) following the manufacturers protocol with minor modifications. UCE were enriched following the myBaits protocol 5.01 (Arbor Biosciences). The enriched UCE libraries were then sent to Novogene Co. Ltd. for sequencing using the Illumina NovaSeq platform with 150 bp paired-end reads.