GSM9378788: NK cells from young donor2; Homo sapiens; RNA-Seq
Total RNA was extracted from young and old primary NK cells RNeasy kit according to the manufacturer´s instructions (Qiagen). For transcriptome analysis, 1 µg of total RNA from young and old NK cells was used. 1 µg of total RNA from young and old NK cells was first rRNA depleted using either Ribominus Eukaryotic system v2 kit (ThermoFisher Scientific). rRNA depleted RNA was subjected to preparation of Illumina compatible RNA-seq libraries using NEBNext® Ultra™ II Directional RNA Library Prep Kit (NEB, Cat # E7760L).