GSM9610830: Frontal cortex, CORT+DFP+LPS, 24 h, A276; Mus musculus; RNA-Seq
Total RNA was extracted from hippocampus and frontal cortex tissue using the E.Z.N.A. RNA/DNA Isolation Kit (Omega Bio-tek). For RNA-seq, poly(A)-selected, stranded mRNA libraries were prepared with the Illumina TruSeq Stranded mRNA Library Prep Kit following the manufacturer's protocol. Whole-genome mRNA sequencing was performed on an Illumina NovaSeq 6000 using paired-end 150 bp reads.