16S rRNA gene amplicon sequencing of soil: Zijinshan gold_copper mine soil, site S4_3 (heavy contamination, 237 m, pH 6.80)
Surface soil samples (0-10 cm) from Zijinshan gold-copper mine were collected, and microbial total DNA was extracted with a commercial kit. The 16S rDNA V4-V5 region was amplified with primers 515F/907R by PCR (27 cycles, 55C annealing), and PCR products were gel-recovered and quantified. Libraries were constructed via TruSeq DNA PCR-Free Library Prep Kit and sequenced on Illumina MiSeq platform with 2250 bp paired-end sequencing.