The impact of Vibrio harveyi infection on the liver of hybrid grouper (Epinephelus fuscoguttatus × Epinephelus lanceolatus)
The impact of Vibrio harveyi infection on the liver of hybrid grouper (Epinephelus fuscoguttatus × Epinephelus lanceolatus)
Before the start of the experiment, 600 healthy hybrid groupers (with an average body weight of 42.3 ± 2.8 g) were acclimatized in a recirculating aquaculture system (water temperature: 28–30°C; pH: 7.8 ± 0.2) and fasted for 24 hours. Subsequently, the fish were randomly assigned to two treatment groups: the control group (con), which received no treatment; and the Vibrio harveyi-infected group (HW), which was administered Vibrio harveyi via oral gavage. Total RNA was extracted from the liver of hybrid grouper by using the TRlzol Reagent and following the manufacturer's instructions (Life Technologies, California, USA). cDNA libraries were constructed as per the instruction manual of NEBNext Ultra RNA Library Prep Kit.