GSM9433434: JHT-2; Mus musculus; RNA-Seq
RNA was purified from MCMV-M45 tetramer+ CD8+ T cells with the RNeasy Plus Micro Kit according to the manufacturer's protocol (Qiagen). RNA was quantified with a Qubit flex fluorometer (Invitrogen) and the quality was assessed on a Bioanalyzer 2100 (Agilent) using a RNA 6000 Pico chip (Agilent). Samples with an RNA integrity number (RIN) of > 8 were used for cDNA amplification using Smart-Seq V4 Ultra Low Input RNA Kit (Takara). Libraries were made from 1ng of full-length cDNA using the Nextera-XT DNA Sample Preparation Kit (Illumina) according to the manufacturer's instructions. The quantity of the cDNA libraries was assessed with Qubit flex fluorometer (Invitrogen) and the average library size was determined using Agilent's 2100 Bioanalyzer HS DNA assay