GSM8942722: G20-2; Helianthus annuus; RNA-Seq
( 1 ) NanoDrop 2000 spectrophotometer : detection of RNA purity and concentration ;( 2 ) Agient2100 / LabChip GX detection : Accurate detection of RNA integrity. ( 1 ) Eukaryotic mRNA was enriched by magnetic beads with Oligo ( dT ) ;  ( 2 ) Fragmentation Buffer was added to randomly interrupt mRNA ;  ( 3 ) The first and second cDNA strands were synthesized using mRNA as template, and cDNA was purified.  ( 4 ) The purified double-stranded cDNA was subjected to terminal repair, A tail and ligation of the sequencing adapter, and then the fragment size was selected with AMPure XP beads ;  ( 5 ) Finally, the cDNA library was obtained by PCR enrichment.