GSM8461831: si-SCR_catabolicSLAM_0hPost-s4U; Homo sapiens; RNA-Seq
At T=0, 3, 6, and 12hr post addition of U-containing media, the medium was removed, the cells were washed twice with cold PBS, and the cells were directly lysed with 350ul TRIzol®-LS. Cells were harvested with Trizol-LS. RNA was isolated and IAA-treatment was performed according to SLAM-seq catabolics module protocol (Lexogen 062.24). Residual genomic DNA was removed using RQ1 RNase-free DNAse (Promega M6101), purified with RNA concentrator (Zymo R1015) RNA-seq library was prepared using TruSeq Stranded mRNA Library Prep Kit (Illumina 20020596).