GSM8894925: Arabidopsis, fas2-4_H3.1_3xFlag, 12DAG, Rep1; Arabidopsis thaliana; ChIP-Seq
Tissue was fixed with 1% formaldehyde for 12mins and stopped by adding glycine to a final concentration of 0.125M and incubating for 6 extra mins.After nuclear lysis,chromatin was sonicated using using Bioruptor® Pico with the following conditions: 14 cycles,10 sec ON/30 sec OFF at 4°C. ChIP-Seq libraries were prepared using Ovation® Ultralow System V2 Library Preparation Kit (PART NO. 0344, 0344NB).