GSM9860534: SIC3; Mus musculus; RNA-Seq
Whole jejunum tissue was harvested and homogenized. Total RNA was extracted using the RNeasy Plus Mini Kit (Qiagen) according to the manufacturer's instructions. RNA quantity and quality were assessed prior to library preparation. Sequencing libraries were prepared from 250 ng total RNA using the Illumina Stranded mRNA Ligation (ISML) library preparation protocol (Illumina protocol 1000000124518 v03). Library concentration was measured using the Qubit dsDNA High Sensitivity assay, and fragment size distribution was assessed using an Agilent TapeStation 4200. Libraries were sequenced on an Illumina NovaSeq 6000 platform using single-end 100 bp reads.