RNA-Seq of human PBMCs: basal, agarikon mycelium
Extracted samples proceeded to sequencing if they had a total RNA quantity of 100 ng and a spectrophotometric A260/280 ratio of 1.95 or greater. Libraries were prepared using poly(A) mRNA enrichment and sequenced by Novogene on an Illumina NovaSeq X Plus platform (paired-end 150 bp; approximately 6.0 Gb raw data per sample). At least 20 million paired-end read pairs were generated for each sample and aligned to the Homo sapiens reference genome GRCh38 (hg38).