Genome sequencing of Streptomyces sp. E-15
The strains were cultured aerobically at 30C for 7 days using YS agar medium. The composition of the agar medium per liter of MilliQ water includes 2g of yeast extract, 10g of starch, and 20g of agar powder. Following a 7-day incubation period, a single colony was transferred to 5 mL of TSB liquid medium supplemented with nalidixic acid and cultured for 4 days. Later, 1 mL of this culture was inoculated into 50 mL of TSB liquid medium with nalidixic acid and incubated for another 4 days. Subsequently, the cells were collected and pelletized, and the genomic DNA was extracted using NucleoBond HMW DNA kit (Macherey-Nagel, Dren, Germany) according to the manufacturers protocol. Long read sequencing libraries were prepared using Rapid Barcoding Kit (SQK-RBK114.24, Oxford Nanopore Technologies) and sequenced on the GridION device using R10.4.1 Flowcell (FLO-MIN114, Oxford Nanopore Technologies). Sequenced reads were basecalled, demultiplexed, and trimmed for the adaptor sequences using Guppy 6.5.7 (super accurate mode).