GSM8513540: RNA from WT E. coli (grown at 45 °C), biological replicates 1 and 2 combined; Escherichia coli; RNA-Seq
RNA was isolated using the PCI protocol (Sambrook & Russell, 2006) with some modifications (Petrov et al., 2022). Nanopore MinION sequencing libraries were prepared with tRNA/rRNA-depleted polyadenylated RNA following the manufacturer's protocol (ONT #SQK-RNA002).