GSM8460943: 16189X7 C3M3 WT; Mus musculus; RNA-Seq
Total RNA was extracted using RNA Stat60® (Tel-Test Inc, USA) following standard phenol-chloroform procedure, and further purified using the NucleoSpin RNA-II purification kit (Machery-Nagel) following the manufacturer's instructions. The quality of the RNA was assessed using the Qubit RNA BR Assay Kit and RIN between 8-10 was recorded. PolyA RNA enrichment was performed using the NEBNext Ultra II Directional RNA Library Prep (New England Biolabs) as per the manufacturer's protocol. Prepared libraries were sequenced on the Illumina NovaSeq platform (NovaSeq Reagent Kit v1.5_150x150 bp Sequencing (100 M read-pairs)) at the genomic and bioinformatic core of the University of Utah