Amplicon sequencing of marine metagenome: eDNA from seawater samples targeting COI using universal primers (mlCO1intF/HCO2198)
eDNA extracted from seawater samples was used to generate amplicon libraries targeting the mitochondrial COI gene using universal primers (mlCO1intF/HCO2198). Libraries were prepared with Nextera adapters, indexed, pooled, and sequenced on an Illumina MiSeq platform (2  300 bp).