RNA-Seq of Homo sapiens: non-HD model cell clone3 derived from 201B7
Total RNA was used to synthesize first-strand cDNA with the SMART-seq v4 Ultra Low Input RNA Kit (Clontech). Double-stranded cDNA was prepared, purified, and fragmented using the Nextera XT DNA Library Preparation Kit (Illumina). Sequencing libraries were constructed by PCR amplification with indexed primers. Paired-end sequencing (150 bp) was performed on a NovaSeq 6000 (Illumina). Reads were mapped to the human genome (GRCh38) using the DRAGEN Bio-IT Platform software (version 3.9.3).