GSM8511904: mutant, -Mg, batch2, rep1; Bordetella pertussis Tohama I; RNA-Seq
Culture aliquots of 7 ml were collected by centrifugation, and total RNA was isolated by the hot-phenol method (Moon et al. 2021).  Total RNA was treated with DNase (Turbo DNA-free kit; Life Technologies, Inc.) for 1 h at 37° C, followed by phenol extraction and ethanol precipitation. rRNA wasremoved using the RiboZero kit. Strand-specific Illumina DNA libraries were prepared for Illumina sequencing using the ScriptSeq 2.0 kit (Illumina).