GSM8942870: CTRL1_B2, GEX; Homo sapiens; RNA-Seq
Miltenyi Nuclei Extraction Buffer (Miltenyi Biotec) with gentle MACS Dissociation and C tubes. Nuclei from day-75 cortical organoids (3 control libraries Ctrl 1-3 and 3 SMS libraries SMS 1-3, n=5 organoids per library, all snRNA-seq libraries were derived from female samples) were isolated using the Miltenyi Nuclei Extraction Buffer (Miltenyi Biotec), following the manufacturer's guidelines with gentle MACS Dissociation and C tubes. Upon isolation, the nuclei were counted on the Nexcelom Cellaca MX. Single nuclei RNA libraries were generated using 10X Genomics' Single Cell Gene Expression kits (RNA-seq and ATAC bundle for Ctrl2, Ctrl3, SMS2 and RNA-seq for Ctrl1, SMS1, SMS3) and loaded onto the Chromium instrument for downstream analysis and processed according to the standard manufacturer's specifications.