GSM9609599: RPMI8226 H3K4me1 ChIP-seq control Input; Homo sapiens; ChIP-Seq
20 million cells per sample were fixed with 1% paraformaldehyde, quenched with glycine and lysed following the SimpleChIP® protocol (Cell Signaling Technology) Chromatin was sheared by sonication, and the mixture was centrifuged to isolate DNA. The DNA was then incubated overnight with antibodies, and antibody-DNA complexes were captured using beads (Active Motif), followed by elution and decrosslinking with proteinase K. The immunoprecipitated DNA was purified with a MinElute PCR Purification Kit (Qiagen) and used for library preparation at NovogeneAIT Genomics (Singapore). Quality control included agarose gel electrophoresis, NanoPhotometer® spectrophotometer (IMPLEN) for DNA purity, and Qubit® 3.0 Fluorometer for measuring concentration. Pair-end sequencing was performed on an Illumina platform, with libraries assessed via Agilent Bioanalyzer 2100.