Targeted SMAD6 sequence data from Langerhans cell histiocytosis cases
Next-generation sequencing libraries were generated using a custom QIAseq Targeted DNA Panel from Qiagen, catalog number 333525. 40 ng of genomic DNA for each sample was fragmented, end-repaired, and A-tailed. Following adapter ligation, target enrichment PCR was performed with a universal primer and 847 custom SMAD6 primers.  This was followed by a universal PCR to add platform-specific adapter sequences. The region of interest covered by the SMAD6 custom panel was 80,630 bp, including 1,036 bp upstream and 1,318 bp downstream of the coding region.