GSM9573013: Astyanax mexicanus larvae, SFLL, rep1; Astyanax mexicanus; RNA-Seq
Pools of 10 larvae at 7.5 dpf from each experimental group were flash frozen in liquid nitrogen and stored at –80°C until further processing. Total RNA was extracted using Qiagen AllPrep DNA/RNA/miRNA Universal Kit (Qiagen, USA, cat # 80224), following the manufacturer's protocol. The quality and quantity of extracted RNA were assessed using gel electrophoresis, an Agilent Bioanalyzer, and fluorometry with a Denovix spectrophotometer and DeNovix RNA Quantification Kit Assay (DeNovix, USA, cat # KIT-RNA-2-NS). 500 nanograms of total RNA were used for library preparation with the Lexogen CORALL RNA-Seq V2 Library Prep Kit with poly(A) RNA selection (Lexogen, Austria, cat # 177.96), including an additional rebinding step to minimize ribosomal RNA contamination. Prepared libraries were sent to Novogene (Beijing, China), where library quality was assessed and sequencing was performed using an Illumina NovaSeq X Plus platform to generate paired-end reads.