GSM9891815: G8-2-5-1; Mus musculus; RNA-Seq
Total RNA was extracted from cell pellets using TRIzol reagent following manufacturer's instructions. RNA purity was measured by Nanodrop 2000, and RNA integrity was verified with Agilent 2100 Bioanalyzer (RIN > 7.0). PolyA mRNA was enriched from total RNA using oligo(dT) magnetic beads. RNA-seq libraries were constructed with Illumina TruSeq RNA Library Prep Kit. Enriched mRNA was fragmented into 200–300 bp short fragments, reverse-transcribed to first-strand cDNA, then synthesized to double-strand cDNA. After end repair, A-tailing and adapter ligation, fragments were amplified by PCR and purified for paired-end sequencing.