GSM9433517: hCMEC/D3 cells, C5a-3, 12h; Homo sapiens; RNA-Seq
Cells were homogenized using QIAshredder kits (Qiagen) and RNA was extracted using the RNeasy kit (Qiagen). 1 ng of total RNA was used for construction of sequencing libraries. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.