GSM9893016: H2AK119ub CUT&RUN in ASXL1 (1-1067) 36EQ; Mus musculus; OTHER
Mouse c-Kit+ bone marrow cells were co-transduced with BAP1 and the indicated ASXL1 construct or empty vector. For ChIP-seq samples, chromatin was prepared from collected cells and immunoprecipitated using antibodies against HA for HA-tagged BAP1 or ASXL1. ChIP DNA was purified for sequencing library preparation. For CUT&RUN samples, nuclei were incubated with an antibody against H2AK119ub, followed by targeted nuclease cleavage and recovery of released chromatin fragments. Released DNA was purified for sequencing library preparation. Sequencing libraries were prepared from purified ChIP-seq or CUT&RUN DNA fragments using standard Illumina-compatible library preparation procedures, including end repair, adapter ligation, PCR amplification, and library purification. Libraries were sequenced in paired-end mode on an Illumina platform.