GSM9537960: Het_clone2; Homo sapiens; RNA-Seq
Organoids were washed with PBS twice, then incubated with dissociation buffer (HBSS with 30U/ml papain and 25U/ml TURBO DNase) at 37° for 20min. Cell were washed and resuspended in DPBS with 0.04% BSA. Libraries using the Chromium Single Cell 3′ Reagent Kits (v.3.1) were prepared following the 10× user guide.