Control (CRX2057057; CRA031066)
The cell suspension was adjusted to ensure the cell density was greater than 1000 cells/microL. Cells were barcode-labeled and mixed with reverse transcriptase into single-cell Gel Beads-In-Emulsions (GEMs), then the cDNA library was constructed by reverse transcription and global amplification in a Mastercycler Pro thermocycler