high dosages24
Total DNA was extracted from mouse fecal samples. Further, the DNA was subjected to high-throughput sequencing where the V4 to V5 regions of 16S rRNA genes were sequenced with an Illumina HiSeq 2500 sequencer (Illumina, Inc., San Diego, CA, US). Data processing and analysis were conducted in accordance with previous studies(Li et al., 2022; Zhang et al., 2021)