GSM9500558: mRNA E18.5 heart Lpar4 WT pup 4; Mus musculus; RNA-Seq
Embryos were collected at E18.5 and dissected tissue was flash frozen in liquid nitrogen. Frozen tissue was transferred to a 50 mL conical tube, 1–2 mL of TRI Reagent was added, and the tissue was homogenized using a TissueRuptor and disposable probes (Qiagen). Following resuspension and/or homogenization, samples were phase separated with 100 μL 1-bromo-3-chloropropane (Sigma). cDNA was prepared from total RNA using a poly(dT)VN primer. Following second strand synthesis, tagmentation, library indexing, and amplification, libraries were sequenced on the Illumina NovaSeq X Plus platform.