NNV-12 (CRX865804)
The total DNA of the  fish Intestinal contents was extracted (n= 3) using a QIAamp DNA mini kit (QIAGEN, Germany) . Agarose gel electrophoresls and DNA concentration (Nanodrop 2000 ThermoScientific) detection were carried out to ensure that the DNA quality met the requirements. PCR amplificatlon was performed using the v3-v4 region-specifc primers 343F(5-TACGGRAGGCAGCAG-3)and 798R( 5-AGGGTATCTAATCCT-3) of 16s rRNA according to the sequencing requirements of Illumina Miseq. The raw data obtained by Illumina MiSeq sequencing was de-Interleaved and spliced.