Isoetes maritima transcriptome
We used a mixture of mature sporophylls and corm tissues as RNA material to sequence the broader range of transcripts presented in the plant. The RNA was extracted two weeks after the collection using RNeasy Mini Kit (Qiagen) with Plant RNA Isolation Aid Buffer (Invitrogen). RNA concentration was measured in the Qubit fluorometer. Transcript library preparation was performed using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) following manufacturer instructions. The library was sequenced on Illumina Hiseq4000 with 150 bp read length.