CKI_IMQ_3 (CRX2065877; CRA031368)
We collected bone marrow derived macrophages from lyz2 cre-folr2 f/f mice and induced them into macrophages using L929 induced media for 7 days. Next, cells were treated with IMQ.  After total RNA extraction using Trizol reagent (TaKaRa, Japan), mRNA was enriched using mRNA Capture Beads, followed by fragmentation, first-strand cDNA synthesis with reverse transcriptase, second-strand cDNA synthesis with end repair and A-tailing, adapter ligation, purification with Hieff NGS DNA Selection Beads, PCR library amplification, and sequencing on Illumina Novaseq X Plus.