Small Genome Sequencing
Total genomic DNA was used for library preparation. DNA was enzymatically fragmented and ligated with MGI-compatible adapters containing unique barcodes. Libraries were amplified by PCR, circularized, and converted into DNA nanoballs (DNBs). Sequencing was performed on the MGI DNBSEQ-G400 platform using paired-end 300 bp reads (PE300). Raw reads were quality filtered using fastp and used for chloroplast genome assembly and characterization of Allium cepa L. Aggregatum Group.