RNA-seq of chorion: COVID-19 uninfected controls
Total RNA was extracted from 134 paired tissue samples using TRIzol reagent (Invitrogen). Sequencing libraries were prepared following the manufacturers protocol with the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs). Libraries were sequenced on the Illumina NovaSeq 6000 platform, generating approximately 6 gigabases of 150-base pair paired-end reads per sample.