ChIP-seq of PD-NC4:clone D1_6mo_input
Chromatin was extracted from 1108 PD-NC4 nuclei and prepared for CENP-A ChIP-seq followed by DNA extraction. Llibraries were prepared using NEB Ultra II (Cat #E7103L). DNA was end-repaired and A-tailed and NEBNext adaptors (Cat #E7335S) were ligated. Libraries were PCR-amplified using only 5-7 PCR cycles and size selected for 200-350 bp.