GSM8996741: sh-caf1-H3.2-ChIP rep2; Drosophila melanogaster; ChIP-Seq
Tissues were dissected in ice-cold PBS and fixed in 1.8% PFA. Samples were sonicated and protein-DNA complexes were immunoprecipitated. DNA was purified by using Phenol/Chloroform and precipitated by using ethanol. Libraries for ChIP-Seq were prepared by using TruePrep DNA Library Prep Kit V2 for Illumina® (Vazyme, CAT#: TD502/TD503). The libraries were purified by RNAClean XP beads and sent to Novogene for quality control and sequencing.