GSM9785761: 012_Week_8; Homo sapiens; RNA-Seq
Total RNA concentration and quality were determined using Qubit fluorometry (ThermoFisher Scientific, Waltham, MA) and the Agilent Fragment Analyzer (Santa Clara, CA). The total RNA underwent globin depletion using GLOBINclear (ThermoFisher Scientific, Waltham, MA) and a clean-up with DNase I (Zymo Research, Irvine, CA) treatment. cDNA libraries were prepared using 100 ng of total RNA according to the manufacturer's instructions for the Illumina Stranded mRNA Prep, Ligation (Illumina, San Diego, CA). The concentration and size distribution of the completed libraries were determined with the Agilent TapeStation and Qubit fluorometry.