GSM8634266: AR2-1; Mus musculus; RNA-Seq
Snap frozen heart tissues were ground to powder under liquid nitrogen. Pulverized samples were used for total RNA extraction using TRIzol reagent (Thermo Fisher Scientific, USA), and RNA purity was checked using the NanoPhotometer® spectrophotometer (IMPLEN, USA). Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.