Transcriptome of Pusa Basmati 1 control plants at anthesis
For library preparation, high-quality RNA was utilized. The process started with mRNA enrichment, followed by mRNA fragmentation which created cDNA through reverse transcription. The process involved adapter ligation and PCR amplification to generate sequencing libraries. The sequencing was conducted on the Illumina HiSeq 2500 platform which generated paired-end reads with a length of 150 bp. The raw image data was processed to create high quality sequence reads which enabled further analysis.