CUT&RUN of IMS13q:clone B2
CUT&RUN for CENP-A was performed with 1 x106 cells. Cells incubated with CENP-A antibody (ab13939, Abcam) o/n at 4C, rabbit IgG (ab46540, Abcam) for 1 h at 4C, pA/G-MNase (Epicypher) at 4C for 1 h. Chromatin fragments were prepared with NEB Ultra II library preparation kit (Cat #E7103L). Samples were amplified for 12 PCR cycles then size selected for fragments of 275-350 bp.