GSM9107372: mESC_EZH2-WT,  rep2; Mus musculus; RNA-Seq
RNA was isolated from cells using the NucleoSpin RNA Kit (Machery-Nagel) as per the manufacturer's protocol. mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. Following fragmentation, first strand of cDNA was synthesised using random hexamers, followed by second strand synthesis using dTTP. cDNA was subject to end repair, A-tailing, adapter ligation before being size selected and amplified for final library generation. Library was assessed by Qubit, Bioanalyser and q-rtPCR for quality control and quantification.