CD22 CART final product #26
Cryopreserved CAR-T cells were thawed at 37C and resuspended in the sample buffer (BD Biosciences). Single-cell capture, and cDNA library preparation were performed using the BD Rhapsody Express Single-cell analysis system (BD Biosciences) according to the manufacturers instructions.  An AbSeq panel containing 30 surface markers and a customized immune response panel (398 genes) were employed. Paired-end sequencing was conducted on Illumina Nextseq 550 platform.