GSM8460916: 5xFAD Cortex microglia rep 3; Mus musculus; RNA-Seq
Cortices were dissected from animals and frozen. Frozen cortices were lysed and microglial mRNAs were isolated using the TRAP protocol cDNA was generated using Nugen Ovation V2 kit. Libraries were produced using TruSeq DNA Sample kit. Multiplexed libraries were directly loaded on NextSeq 500 (Ilumina) with High Output single read sequencing for 75 cycles for RNA seq.