GSM8895348: cKO 2; Mus musculus; RNA-Seq
Total RNA was purified using TRIzol Reagent (15596018, Invitrogen) according to the manufacturer's instructions. Extracted RNA was incubated with DNase I (2270B; Takara, Shiga, Japan) to degrade the genomic DNA and further purified using RNeasy Mini Kits (74104; QIAGEN). The quality of RNA was initially assessed by electrophoresis on a 1.5% agarose gel, and subsequently by absorption spectrophotometry. RNA libraries for RNA-seq were prepared using SMART-Seq v4 Ultra Low Input Kit following manufacturer's protocols.