scRNA-seq of human embryonic stem cell 9 iPSC
Cells were dissociated at day33 with dispase (Worthington, following lihttps://www.sciencedirect.com/science/article/pii/S1934590916301941#section-cited-bynk), cryopreserved. ESC were captured from freshly dissociated colonies, while all differentiated cryopreserved cells were thawed, depleted for dying cells with AnnexinV microbeads (Mitlenyii, Dead cell removal kit), and debris was floated with a density cushion (4% BSA in L15, adjusted to pH7). Cells were hashtagged, pooled, and captured on 10x Chromium 3 Gene Expression ~20K paired end reads/cell following standard methods.