GSM8996715: HD_74A_1 Lane 2; Homo sapiens; ATAC-seq
Cells were harvested using accutase, spun down, and resuspented in 2 mL of PBS for cell counting. Nuclei from 2 x106 cells were isolated following the manufacturer's instructions. The number of nuclei was then assessed by using Countess II. Approximately 16,000 nuclei were incubated with the transposase enzyme, loaded into Chromium Next GEM Chip H Single Cell Kit (10x Genomics), and snATAC libraries were generated with Chromium Single Cell ATAC Reagent Kits User Guide v1.1 (10x Genomics) according to manufacturer's instructions.