16S rDNA microbiome from soil after corn cultivation with bentonite-control 400 kg/ha
DNA quality and concentration were assessed to ensure a minimum of 30 ng of qualified template per sample. The V3-V4 hypervariable region of the 16S rRNA gene was amplified using the primer pair 338F (5-ACTCCTACGGGAGGCAGCAG-3) and 806R (5-GGACTACHVGGGTWTCTAAT-3), as used by (CYTOWANIE). PCR products were purified using Agencourt AMPure XP magnetic beads (Beckman Coulter), dissolved in elution buffer, and labeled for library construction. Library fragment size distribution and concentration were verified on an Agilent 2100 Bioanalyzer. Library preparation, quality control, and sequencing were performed by BGI Genomics (Shenzhen, China) on the DNBSEQ-G400 platform (paired-end 300 bp reads; 30,000 tags per sample).