GSM9108965: Mycelium grown for 7 days in low phosphate medium + one day in high phosphate, sample number 1; Laccaria bicolor; RNA-Seq
Total cellular RNA was extracted from all the samples using Qiagen RNA extraction kit. RNA-seq libraries were prepared from 1 ug of total RNA with the Illumina TruSeq Stranded mRNA reagents (Illumina) using a unique dual indexing strategy, and following the official protocol automated on a Sciclone liquid handling robot (PerkinElmer). Libraries were quantified by a fluorometric method (QubIT, Life Technologies) and their quality assessed on a Fragment Analyzer (Agilent Technologies). Sequencing was performed on an Illumina NovaSeq 6000 for 300 cycles (paired end 150 nt reads) and an average of 40 million reads was obtained per sample.