scRNA-Seq of mus musculus: proximal aorta, 20-week, Male, WT (585/586)
Cells were digested using Liberase (2 units/mL) and Elastase (2 units/mL) in HBSS+, and the suspension was placed in a 14 mL round-bottom tube. The mixture was incubated at 37 C for about 75 minutes, with periodic pipetting up and down approximately 30 times to aid dissociation. Following digestion, the suspension was passed through a 70 m cell strainer, and live cells were sorted from the harvested population. Reverse transcription and amplification yielded approximately 2,2002,500 genes per cell, with a saturation level of 3040%.