Taproot_10yr_R1 (CRX2192993)
After passing quality control, RNA samples were randomly fragmented using a Covaris ultrasonicator, followed by end repair, A-tailing, adapter ligation, purification, and PCR amplification to complete library preparation. Once the libraries met quality requirements, they were pooled according to their effective concentrations and the desired sequencing depth, loaded onto a flow cell, clustered on a cBOT system, and sequenced on an Illumina NovaSeq high-throughput sequencing platform.