D20_B (CRX2039799; CRA030832)
The strain ZCY20-5 was inoculated in D20-B and D20-W medium respectively and cultured anaerobically . The cells were collected by low-temperature high-speed centrifugation when the cells were in a stable phase. The precipitate was quickly frozen in liquid nitrogen and temporarily stored in a refrigerator.Total RNA was extracted from bacterial tissue by CTAB method.