GSM9378763: sham, 72h; Mus musculus; RNA-Seq
We performed single cell separation and library preparation on CD45+ cells using the microwell-based BD Rhapsody scRNA-Seq platform with the BD Rhapsody Whole Transcriptome Analysis (WTA) reagent kit (BD Biosciences), following the manufacturer's instructions. Briefly, forty-thousand CD45+ cells from mice of sham or MCAO group were loaded on the BD Rhapsody cartridge for single cell separation, respectively. Within 30 minutes of retrieving cell capture beads, we initiated reverse transcription to prepare cDNA. We then prepared libraries for RNA transcripts, sample tags according to the manufacturer's instructions. Libraries were sequenced on the NovaSeq 6000 (Illumina) to generate 150 bp paired-end reads.