Adult failing heart 8: DNA methyl-seq of  human left atrial cardiac myocyte nuclei
Genomic DNA was extracted from sorted CM nuclei (>25.000) using AllPrep DNA/RNA Micro Kit (Qiagen, 80284). 200 ng of isolated DNA was sheared to ~300 bp using a Bioruptor Pico (Diagenode, 10 cycles, 30 s on, 90 s off, 'low'). Sequencing libraries were subsequently constructed using the NEBNext Methyl-seq Kit (New England Biolabs, E7120), following the Protocol for Standard Insert Libraries.