GSM9377586: WT PHZ 2; Mus musculus; RNA-Seq
For RNA-sequencing, live, lineageneg, c-kithigh, Sca-1neg, CD41neg, CD16/32neg, CD150neg, CD105pos CFU-E cells were directly sorted into extraction buffer (EB) from the PicoPureTM RNA Isolation Kit (Applied Biosystems) and subsequently stored at -80oC. RNA was isolated as described before (Jutzi et al., Leukemia, 2023). 150 bp paired-end library prep and RNA-sequencing on an Illumina NovaSeq 6000 Sequencing System. Each sample was sequenced to a minimum depth of 100 million reads.