ddRAD of Odorrana swinhoana: adult female tongue
Genomic DNA was extracted from tissue samples using Bioman EasyPure Genomic DNA Spin Kit following the manufacturers protocol. Double-digest restriction-site associated DNA (ddRAD) libraries were prepared using the restriction enzymes SbfI and MspI. DNA fragments were size-selected using gel extraction. Unique barcodes were ligated to each sample for multiplexing. Libraries were pooled in equimolar concentrations and sequenced using an Illumina NovaSeq 6000 with 150 bp paired-end reads at Genomics BioSci & Tech Corp. (Taipei, Taiwan).