Metabarcoding of Norwegian potato field soil: oomycete ITS1 sequences
Total DNA was extracted from 45 mL soil samples. Each soil sample and DNA from the appropriate mock control was amplified with 'OITS' PCR primers targeting the amplification of oomycete ITS1 sequences. Samples were indexed in the amplification PCR (1-step) and demultiplexed by the MiSeq.