GSM8655731: water_24h_old_stem_r1; Alternanthera philoxeroides; ncRNA-Seq
Young(1-2 internodes) and old (3-4 internodes) stems were collected at different time points on land or after waterlogged treatment, total RNA of stems were extracted using TRIzol reagent (Invitrogen, USA), the concentration  of RNA were assessed using Qubit (Thermo, USA) and quality of RNA were assessed using a Qsep100 and R1 RNA Cartridge Kit (BiOptic Inc., China). Small RNA-Seq libraries were prepared using the VAHTS Small RNA Library Prep Kit for Illumina V2 (Vazyme, NR811-01) following the manufacturer's instructions, samples were prepared from three independent biological replicates.