GSM8829728: Dopaminergic neurons, 20mM (control), biol rep2; Homo sapiens; RNA-Seq
Total RNA was extracted using Trizol reagent (Thermo Scientific) as per the manufacturer's protocol. The quantity and quality of extracted RNA samples were analyzed using RNA 6000 Nano kit on a bioanalyzer (Agilent). A quantity of 500 ng of total RNA samples with RNA integrity number (RIN)>7 were used for library construction using the 3′ mRNA-Seq Library Prep Kit FWD for Illumina (QuantSeq-LEXOGEN) as per the manufacturer's instructions. Amplification was controlled by qPCR for obtaining optimal unbiased libraries across samples by assessing the number of cycles (15) required for amplification of the library. DNA High Sensitivity Kit for bioanalyzer was used to assess the quantity and quality of libraries, according to the manufacturer's instructions (Agilent). Libraries were multiplexed and sequenced on an Illumina Nextseq 500 at the genomics facility of IMBB FORTH according to the manufacturer's instructions.