Pacbio sequencing of Nabtat Ali Date Palm
DNA was sheared with Megaruptor 3 to an average size of (15 - 25 kbp). The sequencing libraries were prepared using Pacbio SMRT BELL PREP KIT 3.0 followed by size selection using Pippin HT (Sage Science, MA, US). Finally, the binding was done either by PACBIO Sequel II Binding Kit 3.2 or REVIO POLYMERASE KIT.