Lv_25_2_GF (CRX2095407)
Total microbial DNA was extracted from L. vannamei midgut content samples using the QIAamp Fast DNA Stool Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. DNA concentration and integrity were assessed using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and agarose gel electrophoresis, respectively. Genomic DNA was sheared using an S221 Focused-ultrasonicator (Covaris, USA) and purified with Agencourt AMPure XP beads (Beckman Coulter, USA). Sequencing libraries were constructed using the TruSeq Nano DNA LT Sample Preparation Kit (Illumina, San Diego, CA, USA).