GSM8829412: scRNA-seq of KPC orthotopic tumor treated with 10 mg/Kg EIPA; Mus musculus; RNA-Seq
Fixed, single cell suspension was prepared from flash frozen KPC orthotopic tumors treated with DMSO or 10 mg/kg EIPA (4 tumors pooled for each sample) using the Chromium Next GEM Single Cell Fixed RNA Preparation kit (10x Genomics) according to the user guide (CG000553, Rev B). Cell dissociation was performed using the gentleMACS Octo Dissociator (Miltenyi). Libraries were constructed using the single-plex Chromium Fixed RNA Profiling kit (CG000477, Rev C). Probes were incubated for 20 hours at 42 oC and 12 cycles were performed for the indexing PCR. Libraries were sequenced with the Element Biosciences AVITI Sequencing platform using the AVITI 2x75 High Output Cloudbreak Freestyle Kit (Element Biosciences).