Illumina MiSeq sequencing of Brianella inopinata strain B1
Tagmentation of genomic DNA, and PCR amplification of tagged DNA were performed using the Nextera system (Illumina). Sequencing libraries were pooled and normalized using bead size selection (SPRI beads, Beckman Coulter). Agilent 2100 Bioanalyzer, with High Sensitivity DNA kit was used to quantitate the pooled library before loading onto an Illumina Miseq. Paired-end 250 nt reads were generated using MiSeq V2 chemistry.