16S Metagenomic Seq of Heat stressed E.rubra: bio_replicate_1
Colonies of the soft coral E. rubra were collected from Eoyudo, Korea, and exposed to thermal stress at 26C or maintained at 16C as controls. DNA was extracted from frozen tissues using a CTAB-based protocol. The V3V4 region of the 16S rRNA gene was amplified following the Illumina 16S Metagenomic Sequencing Library protocol, with library preparation performed using Nextera XT indices. Sequencing was conducted with paired-end 2  300 bp reads on an Illumina MiSeq platform.