Base editing screen of Hprt
Base editing mutagenesis screen was performed on Hprt in male mouse embryonic cells. Loss-of-function mutations were selected with 6-Thioguanine. RNA was extracted using Rneasy (QIAGEN), cDNA was synthesized using PrimeScript RT Kit (Takara), the coding region of Hprt was amplified using Q5 polymerase (NEB). Libraries were prepped from PCR amplicons using Nextera XT Kit (Illumina) and sequenced on MiSeq system (Illumina).