GSM8894903: cbr1D Cellobiose Rep 1; Rhodotorula toruloides; RNA-Seq
Cells were harvested by centrifugation and flash frozen using liquid nitrogen. RNA was isolated using the Zymo Quick-RNA Fungal/Bacterial Kit according to manufacturer's protocols. 7.5ug of RNA was DNase treated with Invitrogen DNA-free kit according to manufacturer's protocol. RNA integrity was checked via agarose gel and Bioanalyzer. RNA were prepared for sequencing using poly-A enrichment and standard Illumina protocols.