EF_16 (CRX2210496)
Total microbial genomic DNA was extracted from soil samples using the E.Z.N.A. Soil DNA Kit (Omega Bio-tek, USA). DNA quality and concentration were checked using agarose gel electrophoresis and a NanoDrop 2000 spectrophotometer. The fungal ITS1 region was amplified using primers ITS1F (5'-CTTGGTCATTTAGAGGAAGTAA-3') and ITS2 (5'-GCTGCGTTCTTCATCGATGC-3'). PCR products were purified using the PCR Clean-Up Kit (YuHua, China) and quantified with Qubit 4.0 (Thermo Fisher Scientific, USA) before sequencing on the Illumina NextSeq 2000 platform.