16S amplicon sequencing from fungal samples
Detailed methods for 16S rRNA gene amplicon sequencing and analysis are also described in Robinson et al. 2021. Briefly: a nested polymerase chain reaction (PCR) was performed on total fungal DNA extracts using 27F and 907R primers (V1-V5 region) followed by modified versions of the 341F and 806R primers (V3-V4 region). Samples were sequenced on the Illumina MiSeq platform (2x300 bp paired-end reads).