GSM9138135: GATA6_siCTL_rep1; Mus musculus; ChIP-Seq
cells were trypsinised, MEFs were adsorbed on non-gelatinized plasticware for 20 minutes. Cells were washed once in PBS, pelleted and fixed in PBS-2 mM DSG for 50 min followed by 10 min in PBS-1%PFA. Chromatin Immunoprecipitation was carried out against GATA6 and SOX17as described in material and methods. Libraries were constructed using the NEBNext Ultra II DNA library prep kit using 0.6 μM annealed adapters for ligation. Custom designed Y/forked adapters based on Illumina Truseq indexes with 6-8nt UMI 3' of the index sequence were used