RNA-seq of soybean treated waterlogging condition
RNA-seq of soybean treated waterlogging condition
Roots were collected from three replicates of control plants (con) and plants subjected to 10 days of waterlogging stress (FT), and immediately frozen in liquid nitrozen. Plants of soybean were grown in plastic pots until they reached the V2 stage in an incubator [14-h light/ 10-h dark, 28'C (day)/24'C (night), humidity 60%]. To treat waterlogging stress, the water level to submerge the soil was maintained for 10 days, followed by a recovery time of 15 days. Total RNA was extracted using the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany). Library for RNA-sequencing was constructed by TruSeq Stranded mRNA library prep kit following the manufacturer's instruction