Raw sequence reads from enriched culture
Total genomic DNA was extracted using the DNeasy PowerSoil kit. Native barcode ligation was performed using the Blunt/TA Ligase NBD114.96 kit (Oxford Nanopore). Samples were pooled and adapters ligated using the NEBNext Quick Ligation Module. The library was sequenced on an Oxford Nanopore PromethION (FLO-PRO114-M flow cell) to generate whole-genome metagenomic reads.