Female Control Hypothalamus 4
Hypothalami were dissected. Total RNA was isolated using the RNeasy Mini Kit - Qiagen. High-quality RNA was used for library preparation with the Illumina Stranded mRNA Prep Kit (Cat. #20040534, Illumina), performed by the core facility at the University of Alberta. RNA sequencing was conducted on an Illumina NovaSeq 6000 platform at GIFS/OPAL (Saskatoon, Canada), generating 20 million paired-end (2  150 bp) reads per sample.