Target-enrichment of human skin: Probe type ARGs and MGEs: Enrichment XTHS V2
The sample types used for this experiment included bacteria extracted from human skin, swine skin, and environmental (air) collected by swabs. Each sample taken from either a U.S. swine worker or pen of pigs that each swine worker interfaced with, were subjected to target enrichment using a combined probe-set consisting of probes that are produced in silico: to represent genes from (1) the resistome (RES probes) and mobilome (MOB probes). Each sample was sequenced using an enrichment platform: TELSeq-XT-HS2 Version 2 intended for samples with low gDNA inputs (<200 ng). Library preparation of reads followed accordingly after target enrichment. Samples were subjected to sequencing via either 16S or NovaSeq 6000 shotgun sequencing.