GSM9573916: PMCs, C48/80, 6 hours, biol rep5; Mus musculus; RNA-Seq
For RNA isolation, each sample (0.2 – 2 x 105 PMCs) was lysed in 400 µL RLT Buffer supplemented with 4 μL-Mercaptoethanol. RNA extraction was performed using the RNeasy Micro Kit (Qiagen, Cat. No. 74004). RNA was eluted in 14 µL of buffer, and 10 µL of the eluate was used for library preparation. For samples 1.1–1.3, transcript libraries were constructed using the Ovation SoLo RNA-Seq Library Preparation Kit (NuGEN/Tecan), utilizing 25 ng of cDNA per sample as input. Sequencing was performed on an Illumina NextSeq 2000 platform (EMBL Heidelberg Genomics Core Facility). For samples 2.1–2.4, libraries were prepared using the MGIEasy Fast RNA Library Prep Set following ribosomal RNA removal with the MGIEasy rRNA Depletion Kit V1.3. Sequencing was conducted on the DNBSEQ-G400 platform (MGI Tech) using the DNBSEQ-G400RS High-throughput Sequencing Reagent Set (FCL PE100).