GSM8655612: snMultiome sequencing, liver, maternal lean, ATAC; Mus musculus; ATAC-seq
Nuclei were isolated from ~50 mg of flash-frozen adult liver tissue for simultaneous single-nucleus RNA sequencing (snRNA-seq) and single-nucleus ATAC sequencing (snATAC-seq) using the Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Kit (10x Genomics, Cat# 1000283). For more details please see linked manuscript During the reverse transcription (RT) reaction, cDNA tagging was achieved with 16-nucleotide barcodes and 10-nucleotide molecular identifiers. The pre-amplified product was split into two aliquots: 40 μl for ATAC library construction and 35 μl for further cDNA amplification. For snRNA-seq library preparation, 25% of the total cDNA was used to generate gene expression (GEX) libraries. Libraries were prepared and sequenced according to the manufacturer's protocols: snATAC libraries used the 10x Genomics Single Index N Set, and snRNA-seq libraries used the Dual Index TT Set A.  For more details please see linked manuscript