GSM9573077: total_control, eDRG cultured neuron_1; Mus musculus; RNA-Seq
Total RNA was extracted and purified from freshly collected cultured dorsal root ganglion (DRG) neurons using the RNeasy Mini Kit (Qiagen, 74104) according to the manufacturer's instructions. For mitochondrial RNA sequencing (mito-RNA-seq), mitochondria were first isolated from freshly collected cultured DRG neurons. RNA was then extracted and purified from the mitochondrial pellet using the RNeasy Mini Kit (Qiagen, 74104) according to the manufacturer's instructions. Libraries were prepared following the instrcutions from the manufacture. (cDNA-PCR barcoding kit(SQK-PCB112.24))