AOB_amoA_control_12days
The ammonia monooxygenase subunit A (amoA) gene of ammonia-oxidizing bacteria (AOB) was amplified using the bacterial-specific primer pair amoA-1F (5-GGGGTTTCTACTGGTGGT-3) and amoA-2R (5-CCCCTCKGSAAAGCCTTCTTC-3), as described by Rotthauwe et al., 1997 (DOI: 10.1128/AEM.63.12.4704-4712.2055).