mepp (CRX2201338)
Genomic DNA was isolated from tissues using the Universal Genomic DNA Extraction Kit (Magnetic Bead Method; Maggi Yuhua, China) following the manufacturer's protocol. DNA was sheared to ~400 bp (Covaris) and subjected to end repair, A-tailing, and adapter ligation. Adapter-ligated fragments were PCR-enriched and then hybridized in solution with biotinylated capture probes. Targeted fragments were recovered using streptavidin magnetic beads and further amplified. Final libraries were purified (AMPure XP; Beckman Coulter) and quality-controlled (Agilent Bioanalyzer 2100).