RNA-Seq
Eukaryotic mRNA is enriched using magnetic beads coated with Oligo(dT); Fragmentation Buffer is added to randomly fragment the mRNA; Using the mRNA as a template, the first cDNA strand and the second strand are synthesized, and cDNA purification is performed; The purified double-stranded cDNA undergoes end repair, A-tailing, and ligation of sequencing adapters, followed by fragment size selection using AMPure XP beads; Finally, the cDNA library is obtained through PCR enrichment.