GSM9025124: 201209SHIN#10; Mus musculus; RNA-Seq
RNA was harvested using Rneasy mini plus kit (Qiagen)_Human. For RNA extraction from mouse tissue, we followed the manufacturer's portocol using Trizol reagent(Invitrogen, Carlsbad, CA) following the manufacturer's guidelines. RNA quantity was measured using the Qubit HS RNA assay (Thermo Fisher Scientific)_Mouse mRNA library preparation (poly A enrichment) , the kit we used was Fast RNA-seq Lib Prep Kit V2 (RK20306). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection._Human.Library preparation was performed using the MGIEasy RNA Directional Library Prep Kit (MGI, Shenzhen, China), and paired-end sequencing (150 bp) was conducted on a DNBSEQ-T7 platform, following the manufacturer's protocol._Mouse