GSM9572015: Bd21-3, Mutant, M0558, juvenile leaf tissue; Brachypodium distachyon; RNA-Seq
Tissue sampling was performed in three independent biological replicates per line. The three replicates from each line were subsequently pooled for total RNA extraction. RNA extraction was conducted by Azenta GeneWiz (Leipzig, Germany). Library preparation was performed using Poly(A) selection and paired-end sequencing (2 × 150 bp) was conducted on an Illumina NovaSeq platform at Azenta GeneWiz (Leipzig, Germany).