Donor-Z-CAREp-6
CAR-T cells from three donors under four treatment conditions (12 total BioSamples; SAMN52625553SAMN52625564) were individually stained with hashtag antibodies and subsequently pooled. The pooled cells were then stained using the TotalSeq-C Human Universal Cocktail (BioLegend, catalogue no. 399905).Single cells were barcoded using microfluidic droplet formation and processed for three types of library preparations: whole transcriptome (GEX), surface protein (CITE), and T-cell receptor (TCR) sequencing. All libraries were pooled and sequenced together in a single lane of the Illumina NovaSeq 6000, generating approximately 3 billion paired-end reads.The FASTQ files contain mixed reads from all 12 BioSamples. Demultiplexing of donor and treatment information was performed computationally based on cell barcode and hashtag antibody data. Therefore, users must download all associated files for analysis. The attached files are not specific to any individual sample.