DNA long read sequencing of Brucella from Onthophagus taurus
Nanopore files were prepared using the Oxford Nanopore Technologies SQK-NBD114 native barcoding kit. Long Fragment Buffer was used to promote longer read lengths. Sequencing was performed on the GridION platform (PromethION 2) using a FLOW-MIN114 Spot-ON Flow Cell , vR10 with a translocation speed of 400bps. Base calling was performed on the sequencing platform using the super-accurate basecalling model, MinKNOW v23.07.5 and Guppy v7.0.9.