RNA-Seq of homo sapiens: SCA3 patient 1-derived fibroblasts replicate 3
Library preparation was performed using NEBNext Ultra II Directional RNA library preparation kit for Illumina with rRNA depletion and 500 ng of starting RNA (n=3 for each library). The Qubit RNA high sensitivity assay (Invitrogen) was used to obtain RNA concentration. The Agilent Bioanalyzer (Fragment Analyzer) was used to determine RNA quality as a RIN score. Libraries with sufficient quantity were loaded into P2 flow cell 1000/2000 by Illumina.