RNA-Seq of Pieris rapae: 4th instar larvae composite abdominal tissue
Each 4th instar larvae was dissected to retain only a composite abdominal tisue containing muscle, fat body, and epidermal tissue. This tissue was homogenized in a glass mortar then RNA was extracted using a Trizol extraction protocol (TRI Reagent, Sigma-Aldrich, St Louis, MO, USA). Integrity was assessed with an RNA chip (Bioanalyzer 2100). Samples were utilized for paired-end, barcoded, and strand-specific library construction with the Illumina UDI Library Prep mRNA protocol (Maryland Institute of Genome Science facility). Libraries were sequenced on a single lane of NovaSeq600 (paired-end, 150bp sequencing).