Microbiome of a PHA/biochar bioremediation technology
. Amplicon libraries were prepared with the Oxford Nanopore 16S rRNA Barcoding Kit (SQK16S11424, Oxford Nanopore Technologies, UK) following the manufacturers instructions. The pooled library was loaded onto a FLOMIN114 flow cell and sequenced on a GridION device (Oxford Nanopore Technologies, UK). Raw Nanopore reads were basecalled and demultiplexed with Dorado v7.6.7.