GSM8722254: D15 enriched NK cells_ADT_library 1; Mus musculus; OTHER
C57Bl/6 splenic NK cells were enriched by Miltenyi NK cell negative selection kit. All samples were stained with TotalSeq-C DNA Barcoded CD45.1 and CD45.2 antibodies. Each cohort composed 4 adults and 4 infants each individual mouse was labeled with TotalSeq-C DNA-Barcoded hashtags 1-4 (for adult or infant). Cells were washed and equilabrated and combined. Combined cell pool was then labeled with TotalSeq-C cocktail v1.0 CITE libraries were constructed using the Chromium Next GEM Single Cell 5' Reagent Kit v2 (10x Genomics) with a 5' Feature Barcode Kit (10x Genomics) for antibody-derived tag (ADT) amplification according to the manufacturer's instructions. The ADT library pool and scRNA-seq library pool were each sequenced on one lane of an S4 flow cell on an Illumina NovaSeq 6000 (~2.5 billion 2x100 bp reads per lane). Quantification of scRNA-seq library pools was performed by pseudoalignment to the human transcriptome (Gencode v24 annotation derived from GRCh38) using kallisto v0.46.2 in “BUS” mode