2_2 (CRX2129114)
Approximately of leaf tissue from each sample was homogenized in  ice-cold lysis buffer using a glass dounce homogenizer. Followed by centrifugation. The nuclear pellet was resuspended in reaction buffer. Nuclei were subjected to transposition by adding of transposase, followed by incubation  for some times with gentle mixing. The reaction was stopped by adding EDTA-containing buffer, and DNA was purified using the MinElute PCR Purification Kit.Following transposition, DNA was amplified using a limited-cycle PCR with Nextera index primers to generate sequencing libraries. Libraries were cleaned using AMPure XP beads and quantified with a Qubit fluorometer. Library size distribution was assessed on an Agilent Bioanalyzer. Paired-end sequencing was performed on an Illumina HiSeq 2500 platform