TAC-4w
First, the cell nuclei are extracted from the tissue through grinding. The 10x Genomics platform uses microfluidic technology to sort individual cell nuclei. Then, gel beads (Gel Beads) carrying cell nucleus tag information (barcode) and primers, along with individual cell nuclei, are encapsulated in oil droplets to form an oil-in-water structure (Gel Bead-in-Emulsion, GEMs). Within the oil droplets, the gel beads dissolve to release reverse transcription reaction reagents, and the cell nuclei are lysed to release mRNA. cDNA with barcodes for sequencing is obtained through the SMART method. After the liquid oil layer is disrupted, the cDNA undergoes subsequent library construction and is sequenced using the Illumina sequencing platform (NovaSeq 6000 or NovaSeq X).