Phylogenomics and the evolution of larval feeding habits in the blow flies (Diptera: Calliphoridae)
The sequences were obtained by Anchored Hybrid Enrichment, an exon capture method where fragmented genomic DNA extracts for each fly species are hybridized to a custom set of tiled oligonucleotide probes designed to match orthologous exons in the Diptera genome. Hybridized fragments are isolated by magnetic beads and a library is constructed.  sequencing of pooled libraries is carried out on Illumina MySeq or HighSeq sequencers using single-read in the NCSU Genomic Sequencing Laboratory. Reads are demultiplexed, trimmed, and assembled using Spades 3.11.1 software.