RNA-Seq of Pelodiscus sinensis liver (6.0 ug/L Deltamethrin, replicate 3) (CRX2124044)
Total RNA was extracted from liver tissue using Trizol reagent (Invitrogen, Carlsbad, CA, USA). Subsequently, mRNA was enriched using Oligo (dT) magnetic beads and fragmented in Frag/Prime buffer. The RNA fragments were reverse-transcribed into double-stranded cDNA. Sequencing libraries were then constructed following procedures of purification, end repair, adapter ligation, and PCR amplification. Qualified library products were sequenced on an Illumina NovaSeq platform.