GSM9786034: induced Pluripotent Stem Cells (iPSC), small RNA-seq, R1; Homo sapiens; ncRNA-Seq
Total RNA-seq and small RNA-seq libraries were generated for induced Pluripotent Stem Cells (iPSCs), primordial germ cell-like cells (PGCLCs), xrSpermatogonia (xrSpgn) and xrTestes (total), with three biological replicates per sample. RNA was isolated using the PureLink miRNA Isolation Kit (Thermo Fisher Scientific) according to the manufacturer's instructions. The long RNA fraction (>200 nt) was retained and further purified with TRIzol. Small RNAs were extracted using the same kit in combination with TRIzol, following the manufacturer's protocol. RNA-seq libraries were prepared using the NEBNext Ultra II directional RNA library Prep kit with rRNA depletion following the manufacturer's instructions. Small RNAs for small RNA-seq were prepared for sequencing as described in Konstantinidou et al. (2024).