GSM9892212: Uterine tissue, IUA, 2 weeks, rep3; Rattus norvegicus; RNA-Seq
Total RNA was extracted from rat uterine tissue samples. RNA concentration and purity were subsequently assessed, and RNA integrity was evaluated using an Agilent 2100 Bioanalyzer. During library construction, poly(A)-tailed mRNA was first enriched from total RNA using oligo(dT) magnetic beads. The enriched mRNA was then fragmented into approximately 300 bp fragments by ion-mediated fragmentation. cDNA was synthesized using RNA as the template. After PCR amplification, libraries with an approximate size of 450 bp were selected. Library quality was then assessed using an Agilent 2100 Bioanalyzer.