GSM9238601: Epitheilal vehicle control replicate 3; Bos taurus; RNA-Seq
RNA extraction and sequencing Following treatment cells were washed with PBS, lysed in 400µL mirVana lysis solution from mirVana RNA extraction kit (Invitrogen, St. Louis, US) and snap frozen. RNA was extracted from the cell pellets using the MirVana RNA extraction kit (Invitrogen, St. Louis, US) as per manufacturer's protocol. Genomic DNA removal was carried out using the DNA free kit (Thermo Fisher Scientific, Massachusetts, US) as per the manufacturer's protocol. RNA libraries were sequenced at the NGS Facility at the University of Leeds where they were prepared and sequenced as per their standard protocols using the Illumina TruSeq Stranded Total kit according to manufacturer's guidelines. The RNA libraries were sequenced using the Illumina NextSeq 500 machine (Illumina, California, USA) with a single end 75 bp length read.