GSM9788392: Human DC 8h post-infection; Homo sapiens; RNA-Seq
Library extraction was performed as per BD Rhapsody's protocols (Doc ID: 23-24120 Rev. 1.0) for their WTA + SMK + Abseq kit At each timepoint, a separate BD Rhapsody cartridge was used. For each cartridge, Abseq + SMK- stained cells from each donor and condition were pooled in equal numbers before processing via the BD Rhapsody Express system. A total count of 40,000 cells was attempted for each cartridge. Library preparation was performed as per manufacturer's protocols (Doc ID: 23-24120 Rev. 1.0). Final sequencing libraries were pooled and sequenced on a single lane of Novaseq X, together with 20% PhiX spike in as per BD's recommendations.