Rla_survey
Libraries were constructed by MGIEasy Universal DNA Library Prep Kit V1.0CAT#1000005250, MGIfollowing the standard protocol. Briefly, 1g genomic DNA was randomly fragmented by Covaris. The fragmented DNA was selected by MGIEasy DNA Clean beads (CAT#1000005279, MGI) to an average size of 200-400bp. After that, the selected fragments were end-repaired, 3'adenylated and then ligated adapters. The DNA samples were amplified by PCR and the products were purified by the MGIEasy DNA Clean beads (CAT#1000005279, MGI). The double stranded PCR products were heat denatured and circularized by the splint oligo sequence in MGIEasy Circularization ModuleCAT#1000005260, MGI. The single strand circle DNA (ssCir DNA) were formatted as the final library and qualified by QC. The qualified libraries were sequenced on DNBSEQ-T7 platform.