GSM9433556: 786-O_H4K12la_DEX_rep1; Homo sapiens; OTHER
CUT&Tag was performed using the NovoNGS CUT&Tag 4.0 High-Sensitivity Kit (for Illumina) (Novoprotein) according to the manufacturer's instructions. Briefly, 786-O cells were harvested and immobilized on Concanavalin A–coated magnetic beads. Cells were incubated sequentially with specific antibodies against H4K12 lactylation or GR and a secondary antibody. Subsequently, pA-Tn5 transposase was added to bind antibody-labeled chromatin. After transposase activation and tagmentation, genomic DNA fragments were released, amplified, and purified for library preparation. The library for sequencing was constructed and NovoNGS DNA Clean beads were used for purification steps. The library was quantified with NovoNGS CUT&Tag 4.0 High-Sensitivity Kit (for Illumina) and sequenced on an Illumina novaseq 150PE.