tRNA-seq uMRT_C
Silica column extracted (C) tRNA, followed mim-tRNA-seq library preparation workflow, reverse transcribed by UltraMRT (uMRT_C). Sequencing was performed in paired-end mode on an Illumina platform. Due to short tRNA insert sizes, only Read 1 was used for downstream analysis.  Secondary demultiplexing (RT-adaptor indices) of the individual RT reactions was preformed by user according to mim-seq workflow.