ATAC-NSN-rep1 (CRX2069149)
Library preparation was performed as previously described99 after minor modifications. 50 mouse oocytes (per sample) were treated with acidic Tyrode's solution (Sigma-Aldrich) to remove the zona pellucida and transferred into 500 ul of ATAC-Resuspension Buffer [10 mM Tris-HCl (pH 7.4), 10 mM NaCl and 3 mM MgCl2] containing 0.1% NP-40, 0.1% tween-20 and 0.01% digitonin to lyze for nuclei using a glass capillary with <50 um opening. The purified libraries were quantified by Qubit 1X dsDNA HS Assay Kit and qPCR, analyzed by a Fragment Analyzer System to confirm successful amplification and quality, and sequenced using Novaseq 6000 Sequencing System.