GSM9078238: Lung, Single dose BLEO-IPF Vehicle, Rep 2; Mus musculus; RNA-Seq
The inferior lobe samples were homogenised in lysis buffer and RNA was purified using NucleoSpin® RNA binding strips (MACHEREY-NAGEL). RNA sequence libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, USA) and sequenced on the NextSeq 500 (Illumina) with NextSeq 500/550 High Output Kit V2 (75 CYS, Illumina, San Diego, USA).