16Smet metabarcoding lab DNA extraction control Prefilter: lab quality control
An empty 5 mL screw cap tube prefilter extraction control was extracted with PowerSterivex kit reagents with only reagents added. Primers targeting the 16S mitochondrial gene (Kelly et al. 2016) were used to generate amplicons sequenced on an Illumina MiSeq with v3 chemistry generating paired-end reads. Samples were dual indexed with Nextera indices following the Illumina 16S metagenomics protocol (Illumina Part# 15044223 Rev B).