Larval fathead minnows exposed for  24 h to  fluticasone propionate
Libraries were prepared using the Lexogen QuantSeq 3 mRNA-Seq V2 Library Prep Kit with UDI (Unique Dual Index) and QuantSeq UMI (Unique Molecular Identifier) module. Preparation followed manufacturers recommendations. Completed libraries were QCd and quantified using a combination of Qubit dsDNA HS and Agilent 4200 TapeStation HS DNA1000 assays. Libraries were pooled in equimolar amounts and the pools were quantified using the Invitrogen Collibri Quantification qPCR kit.