GSM9262121: p53knockout and ATRXknockout_mNPC H3K4me3 ChIP-seq; Mus musculus; ChIP-Seq
Chromatin immunoprecipitation followed by sequencing (ChIP-seq) was conducted as previously described (PMID: 34059508). The chromatin shearing conditions were optimized for primary murine neuroepithelial progenitor cells and tissues. The antibodies used in this procedure included H3K9me3 (ab8898), H3K4me1 (ab8895), H3K27ac (ab4729), H3K4me3 (ab8580), H3K79me2 (ab3594), and H3K27me3 (ab6002). Chromatin immunoprecipitation DNA was used to make library with NEBNext Ultra II DNA Library Prep Kit using kit manual.