Metformin 3.0M
Equal volumes of up to 24 samples (amplified with unique i5/i7 index primer combinations) were pooled and a 0.55-0.75X double-sided SPRI bead clean-up was performed. Libraries were quantified using a Bioanalyzer 2100 (Agilent) and sequenced at 150bp PE on MiSeq Nano.