ITS-Seq of Jivamrit: Location 3
The genomic DNA of NF inputs was isolated using DNeasy Power Soil Pro Kit (Qiagen) by following manufacturer protocol and quantified using Qubit fluorometer v4.0. The V3V4 region of the bacterial 16S rRNA gene was amplified using the primers ILL_HP-16S-F and ILL_HP-16S-R. However, fungal internal transcribed spacer region was amplified using the primers ILL_HP-FN-F and ILL_HP-FN-R. Libraries were prepared using 16S rRNA gene and ITS Amplicon based Metagenomic Sequencing Library Preparation protocol by Illumina.