GSM8635045: Q157R_KL_Native_1; Mus musculus; RNA-Seq
Mouse bone marrow myeloid progenitor cells (cKit+ Sca1- Lin-) were isolated from Mx1-Cre/U2af1(+/+), Mx1-Cre/U2af1(S34F/+), and Mx1-Cre/U2af1(Q157R/+) mice by enrichment of cKit+ cells using cKit microbeads (Miltenyi Biotec, Catalog # 130-091-224) followed by fluroescence-activated cell sorting. Immediately after cell isolation, total RNA was extracted using the NucleoSpin RNA Plus XS Micro Kit (Macherey-Nagel, Catalog # 740990) and eluted in RNase-free water, per the manufacturer's instructions. RNA was stored at -80C until day of bioanalyzer assessment (Agilent) and library construction. The RNA-sequencing libraries were constructed using KAPA RNA HyperPrep Kit with RiboErase (Roche, Catalog # KK8560/61), per the manufacturer's protocol. After amplification, final libraries (~360 bp mean size) were sequenced on Illumina NovaSeq 6000, generating 2 x 150bp paired-end reads.