GSM9433598: PANC-1_Input; Homo sapiens; ChIP-Seq
ChIP assays were performed according to the standard crosslinking ChIP protocol with modifications. Briefly, cells were harvested and crosslinker with 1% formaldehyde for 10 min at room temperature. After sonication, immunoprecipitation was performed with antibody. The immunoprecipitated complex was washed, and DNA was extracted and purified by MinElute PCR Purification Kit (QIAGEN, #28006). The ChIP-Seq library was prepared using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme, #TD501) according to the manufacturer' s instructions. For ChIP-seq, extracted DNA was ligated to specific adaptors followed by deep sequencing in the Illumina Novaseq 6000 using 150bp paired-end.