Rnaseq of R108 roots under CK condition
After the sample passes quality control, library construction is performed. The main procedures are as follows:  mRNA enrichment from total RNA: Magnetic beads coated with Oligo(dT) are used to capture mRNA with poly(A) tails.  Fragmentation and cDNA synthesis: The enriched mRNA is fragmented using a fragmentation buffer. Random N6 primers are then used for reverse transcription to synthesize first-strand cDNA, followed by second-strand cDNA synthesis to generate double-stranded DNA (dsDNA).  End repair, A-tailing, and adapter ligation: The dsDNA is end-repaired, with the 5 ends phosphorylated and a single A nucleotide overhang added to the 3 ends. Bubble-shaped adapters with a 3 T overhang are then ligated to the products.  PCR amplification: The ligation products are amplified by PCR using specific primers.  Singlestrand circularization: The PCR products are heat-denatured to single strands, and a bridge primer is used to circularize the single-stranded DNA, yielding a singlestrand circular DNA library.