1day_control_4
RNA was extracted using the Spectrum Plant Total RNA kit (Millipore Sigma). An on-column DNase I digestion was performed prior to the final wash steps (Sigma-Aldrich). A subsequent RNA sample cleanup was performed using RNA MagClean DX (Aline Biosciences) and eluted into elution buffer. The quality of the resulting RNA was assessed using the Nanodrop 2000c (Thermo Scientific) and a BioAnalyser2100 (Agilent Technologies).