Bos taurus Hanwoo PBMC unstimulated control sample from Animal4
Peripheral blood mononuclear cells (PBMCs) were isolated from clinically healthy Hanwoo cattle. This library was prepared from Animal4 under the unstimulated control condition. For each animal, PBMCs were divided into paired unstimulated and stimulated aliquots. Stimulated samples were incubated for 12 h with a combination of whole cell sonicates from three bovine digital dermatitis-associated Treponema species. Libraries were prepared using the TruSeq Stranded Total RNA Library Prep Gold Kit with rRNA depletion and sequenced as 101 bp paired-end reads on an Illumina NovaSeq 6000 platform.