GSM9892264: LNPIFN-Y4; Mus musculus; RNA-Seq
After the addition of a chloroform:isopentanol mixture (24:1) and centrifugation, the supernatant was transferred to a deep_x001E_well plate pre_x001E_loaded with binding solution and magnetic beads. RNA purification was carried out using an automated nucleic acid extractor, and the purified RNA was eluted with RNase_x001E_free water. For library construction, mRNA was captured using oligo(dT)-conjugated magnetic beads, fragmented, and then reverse_x001E_transcribed into first_x001E_strand and second_x001E_strand cDNA. The resulting double_x001E_stranded cDNA was subjected to end repair, A_x001E_tailing, adaptor ligation, and PCR amplification.Following library quality control, single_x001E_stranded circular DNA was generated and sequenced on a DNBSEQ platform via rolling circle amplification combined with combinatorial Probe_x001E_Anchor Synthesis (cPAS).