DNA long read sequencing of Dysgonomonas from Onthophagus taurus
Nanopore files were prepared using the Oxford Nanopore Technologies SQK-NBD114 native barcoding kit. Long Fragment Buffer was used to promote longer read lengths. Sequencing was performed on the PromethION 2 platform using a FLO-PRO114M Spot-ON Flow Cell, vR10 with a translocation speed of 400bps. Base calling was performed on the sequencing platform using the super-accurate basecalling model, MinKNOW v24.06.10 and Dorado v7.4.12.