GSM8721261: 2063A_0h_RNA_rep2; Brassica napus; RNA-Seq
Toal RNA was extracted by using Eastep® Super Total RNA Extraction Kit (Promega) from cotyledon with three replications. For ATAC-seq, the extracted nuclei were incubated with Tn5 transposase and tagmentation buffer following the manufacturer's instructions (Vazyme, TD501-01). mRNAs with polyA tails were enriched by Oligo(dT) magnetic beads, and mRNAs were obtained by removing ribosomal RNAs from the total RNAs. For ATAC-seq, DNA was purified using the MinElute PCR Purification Kit (QIAGEN, 28004). Genomic DNA for was extracted using the DNeasy Plant Mini Kit (QIAGEN, 69104). Bisulfite-treated DNA libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs, E7645) following the manufacturer's instructions.