RNA-Seq of E. coli : Wildtype strain Replicate2
Aerobic growth in LB medium at 37 degrees celsius, 180 rpm until OD (600nm) = 1.e⁸ cells pelleted and flash-frozen in liquid nitrogen. The RNA preparation was performed by GENEWIZ plateforme : RNA purification, rRNA depletion, cDNA first-strand synthesis and cDNA libraries preparation (Illumina Stranded Total RNA Prep with Ribo-Zero Plus, Illumina). High throughput illumina sequencing with NovaSeq X Plus sequencer.