GSM8996706: CEPT1, endothelial cell-specific,  Cept1 overexpression (Cept1fl/fl Cre+); Mus musculus; RNA-Seq
Male mice were euthanized, and the thoracic and abdominal aorta were surgically micro-dissected from the aortic arch to the distal aortic bifurcation in the pelvis. Aortic tissue (n = 3 per condition) was minced into small pieces using a scalpel and transferred into a glass vial with 1.5 mL of cold digestion buffer (125U/ml Collagenase XI, 60U/ml Hyaluronidase type 1-s, 60U/ml DNase I and 450U/ml Collagenase type I. Tissue was further digested in a shaking (100 rpm) 37 °C water bath for 1 hour. The resulting cell suspension was strained through 70μM cell strainer and treated with ACK lysis buffer to lyse red blood cells. Single-cell suspensions then used for subsequent single-cell sorting. Transcriptional profiling of Cept1fl/fl Cre− and Cept1fl/fl Cre+ mouse whole aortae was performed using droplet-based massively parallel 10X genomic single-cell RNA sequencing (scRNA-seq) 3' v3. Single cells for each genotype were individually barcoded and subjected to sequencing, generating Fastq files.