GSM9612309: GF_PBS, 5 dpf_1; Danio rerio; RNA-Seq
Zebrafish larvae from all groups  groups (n=3) were resuspended and crushed in 0.5 ml of TRIzol Reagent The NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760S/L) was used to process the samples. Briefly, mRNA was isolated from total RNA using oligo-dT magnetic beads. After fragmentation of the mRNA, a cDNA synthesis was performed. This was used for ligation of the sequencing adapters and PCR amplification of the resulting product. The quality and yield after sample preparation was measured with Fragment Analyzer. The size of the resulting products was consistent with the expected size distribution (a broad peak between 300-500 bp).