GSM9611215: RD cells, vincristine-resistant, replicate 2; Homo sapiens; RNA-Seq
RNA was extracted using the Qiagen RNeasy Mini Kit. mRNA was enriched with oligo(dT) beads, fragmented at 94C for 15 min, converted to double-stranded cDNA, end-repaired, adenylated, ligated to adapters, and PCR-amplified with indexed primers.