Postnatal day 6 ear cartilage (CRX2045725; CRA030954)
Single-cell RNA sequencing (scRNA-seq) libraries were prepared using the MobiNova microfluidic system (Novogene, China). Single cells were captured and barcoded in oil-water emulsion droplets, followed by reverse transcription, cDNA amplification, and library construction according to the manufacturer's protocol. Libraries were sequenced on an Illumina NovaSeq 6000 platform with a paired-end 150 bp (PE150) strategy.