Genome sequencing of One Health sourced E. coli
Sample swabs were streaked onto lysogeny broth (LB) agar plates and single colonies collected for culture in 10 ml liquid LB. Following overnight culture, total cellular DNA was extracted using the ISOLATE II Genomic DNA (Bioline) kit following the manufacturers standard protocol for bacterial cells and was stored at 4 C. Library preparation was done by the Australian Institute for Microbiology and Infection Core Sequencing Facility at the University of Technology Sydney, following the adapted Nextera Flex library preparation kit process, Hackflex. See https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9997745/ for more details