GSM9535929: Wildtype non-infected sample; Mus musculus; RNA-Seq
Cell number and viability were analyzed using Nexcelom Cellometer Auto2000 with AOPI fluorescent staining method. Sixteen thousand cells were loaded into the Chromium iX Controller (10X Genomics, PN-1000328) on a Chromium GEM-X Single cell 3' Chip Kit v4 (10x Genomics, PN-1000215), and processed to generate single cell gel beads in the emulsion (GEM) according to the manufacturer's protocol. The cDNA and library were generated using the GEM-X Single cell 3' Kit v4 (10x Genomics, PN-1000691) and Dual Index Kit TT Set A (10X Genomics, PN-1000215) according to the manufacturer's manual. Quality control for constructed library was performed by Agilent Bioanalyzer High Sensitivity DNA kit (Agilent Technologies, 5067-4626) and Qubit DNA HS assay kit for qualitative and quantitative analysis, respectively. The multiplexed libraries were pooled and sequenced on Illumina Novaseq X Plus sequencer with 100 cycle kits using the following read length: 28 bp Read1 for cell barcode and UMI, and 90 bp Read2 for transcript.