GSM9302462: MSC, Passage 5, 2kPa substrate, RNA-Seq, rep1; Rattus norvegicus; RNA-Seq
Cells were lysed using TriZol (Thermo Fischer) and RNA isolated with Qiagen's RNeasy Micro Prep kit. RNA isolates with masses >= 0.5μg and RNA integrity number (RIN) scores >= 9.0 were used to create sequencing libraries. RNA libraries were constructed using the TruSeq Stranded mRNA Library Prep Kit (Illumina) according to the manufacturer's instructions.