GSM9238534: astR-2-Succ/NH4; Acinetobacter baumannii; RNA-Seq
Pellets were thawed on ice and resuspended in 100 µL of RNAse free TE Buffer pH 8.0. The samples were lysed and homogenized in a Yellow Bullet Blender tube (MidSci) at Bullet Blender (NextAdvance) speed 8 for 3 minutes. The Qiagen RNeasy was used to isolate and purify the RNA from the samples, following the manufacturer's protocol. Samples were prepared for sequencing using an Illumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus Microbiome and unique dual indexes.