GSM8829385: Murine CAFs treated with 0.2 mM glutamine + 25 uM EIPA, Rep1; Mus musculus; RNA-Seq
Total RNA was extracted using a PureLink RNA Mini Kit (Invitrogen). PolyA RNA isolation and library preparation was performed with the Watchmaker mRNA Library Prep Kit (Watchmaker Genomics: 7BK0001) with xGEN Stubby adaptors (IDT, Cat# 336338420) and xGEN 10nt UDIs (IDT, Cat# 336338436). Libraries were sequenced (2x75bp) with the Element Biosciences AVITI Sequencing platform using the AVITI 2x75 High Output Cloudbreak Freestyle Kit (Element Biosciences, Cat# 860-00015).