Colon cancer
RNA was extracted from FFPE slices or RNA-stabilized solutions using the QIAGEN RNeasy Kit, adhering to the manufacturers protocol. Library construction and ribosomal RNA depletion were performed using the KAPA RNA Hyper with rRNA Erase (HMR only) kit. To multiplex samples in one sequencing run, different adaptors were utilized. Library concentrations were measured using the Qubit dsDNA HS Assay kit (Life Technologies), and quality was assessed with the Agilent Tapestation (Agilent). RNA sequencing was conducted on the Genolab M engine for paired-end sequencing with a read length of 75 bp.