GSM8510781: WT_Vehicle_replicate4; Mus musculus; RNA-Seq
RNA was extracted with RNeasy kit (Qiagen, 74104) with with DNase I treatment (Qiagen, 79254) according to the manufacturer's instructions. PolyA enrichment was performed with NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490S, New England Biolabs), and library preparation with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760S, New England Biolabs). NEBNext Multiplex Oligos (E6440S, New England Biolabs) were used as indexes. Starting input of RNA was 1ug. Libraries were sequenced on the Illumina NovaSeqX platform with 50-bp paired-end sequencing.