GSM8513347: Ldlr KO-4; Mus musculus; RNA-Seq
RNA was isolated from mouse colon tissue with trizol reagnt. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library.