Surface ocean metagenomes from the Hawaii Diel Sampling (HADS).
Surface ocean seawater was prefiltered with 85-m Nitex mesh and then microbial cells were collected on 0.2 m Sterivex cartridge filters. Following DNA extraction, DNA was sheared to 400 bp on a Covaris S220, libraries were prepared using the Ovation Ultralow System V2 Kit, and libraries were paired-end sequenced on an Illumina NextSeq 500.