GSM8605301: SE initiating cell, shFOXO4, ATAC, Rep1; Homo sapiens; ATAC-seq
Cells were harvested and lysed using ice-cold lysis buffer, subsequently, the nuclei were resuspended and allowed to incubate in a transposase reaction mixture (TD501, Vazyme Biotech) at a temperature of 37°C for a duration of 30 minutes. Purification of the transposed DNA fragments was accomplished using the MinElute PCR Purification Kit supplied by QIAGEN. The construction of the ATAC-seq library, which encompassed steps such as end-repairing, adapter ligation, and PCR amplification, was carried out following the manufacturer's instructions using the TruePrep DNA Library Prep Kit (TD501, Vazyme Biotech).