RNA-seq transcriptomics of doxorubicin (DOX) exposure in mouse colonoids (in vitro; 0, 0.1, 10 µM; 24, 48, 72 h), with raw paired-end FASTQ files
RNA-seq transcriptomics of doxorubicin (DOX) exposure in mouse colonoids (in vitro; 0, 0.1, 10 µM; 24, 48, 72 h), with raw paired-end FASTQ files
After exposure (24/48/72 h), Matrigel domes were dissolved on ice with cold PBS and colonoids were pelleted by centrifugation (200 × g, 5–10 min, 4 °C). Pellets were transferred to RNAlater (4 °C overnight) and stored at −80 °C until RNA extraction. Total RNA was extracted using the miRNeasy Mini Kit (Qiagen) following the manufacturer's instructions. RNA quantity/purity were assessed by NanoDrop and RNA integrity was assessed by Agilent 2100 Bioanalyzer; samples with RIN > 6 and sufficient yield were used for library preparation. mRNA sequencing libraries were prepared from total RNA using the Lexogen SENSE mRNA-seq library preparation kit, following the manufacturer's instructions, including sample indexing for multiplexing.