RNA-Seq of mus musculus: adult female esophagus
Total RNA was extracted from mouse esophageal tissues using TRIzol reagent. Ribosomal RNA was depleted using the Ribo-off rRNA Depletion Kit. Libraries were then constructed using the KCTM Stranded mRNA Library Prep Kit for Illumina. The library products were enriched and size-selected to 200-500 bp, followed by quantification and sequencing on the NovaSeq 6000 system (Illumina) using the PE150 strategy, performed by SeqHealth (Wuhan, China).