GSM9108599: PSOR2005.1; Homo sapiens; RNA-Seq
citrated blood was collected, spun, and platelet-rich plasma was collected. ACD was added 1:10 to resting citrated blood samples and spun at 1000g for 10 minutes. The pellet was resuspended in Easysep buffer (STEMCELL) and subjected to negative selection using human CD45+ and Glycophorin A+. A relative purity of platelet/leukocyte ratio of 1x107 was confirmed by flow cytometry. Samples were lysed in TRIzol (ThermoFisher) and stored at -80oC until use.  RNA was extracted using Direct-zol microspin columns (Zymo Research). Yield, quantity, and quality of the RNA were assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). Libraries were prepared utilizing the Clontech SMART-Seq HT with Nxt HT kit (Takara Bio USA), and sequenced single end on an Illumina NovaSeq 6000.