libNP1763_UDS8_SBF2_gT-pD_MutRec_Rep3_figS12C_R1
gDNA is extracted from edited cells by Zymo Quick DNA miniprep. DNA is randomly tagmented with Tn5 to introduce adapters. Nested PCR is performed to amplify the junction between the integrated plasmid donor and the genomic integration locus by amplifying the plasmid and the Tn5 adapter.