WGS of human oral metagenome: SK2D23YNT_V1
DNA was extracted from oral rinse samples using the QIAamp PowerFecal Pro DNA extraction kit. Raw sequencing reads were demultiplexed using bcl-convert (v3.8.2-12-g85770e0b). Quality filtering was performed using fastp22 (v0.23.1) to remove reads with low average quality scores (-e 25) and short lengths (-l 70). The filtered reads were then aligned to the human reference genome (GRCh38.p14) with BWA-MEM25 (v0.7.17) using default parameters to remove host DNA contamination. Paired-end reads, for which neither mate mapped to the human genome, were retained as microbial and uploaded to SRA.