GSM9573963: WT_Tm_ATAC_rep1; Homo sapiens; ATAC-seq
50,000 HCT116 cells were lysed on ice for 10min in lysis buffer containing 10mM Tris (pH 7.4), 10mM NaCl, 3mM MgCl2, 0.1% NP-40, 1% Tween-20, and 0.01% digitonin. Nuclei were subsequently washed with the same buffer lacking NP-40 and digitonin. Tagmentation was performed using the Nextera DNA Library Prep kit [Illumina, FC-121-1030] according to the manufacturer's instructions. The transposed DNA was purified using the MinElute PCR Purification Kit (QIAGEN, 28006). Purified tagmented DNA was PCR-amplified using Nextera PCR primers, followed by size selection and cleanup with AMPure XP beads (0.5x-1.8x).