GSM8862953: RNA-seq, MiaPaCa-2 HIF1α KO cells, Hypoxia, replicate 3; Homo sapiens; RNA-Seq
After treatment, total RNA was isolated using QIAGEN RNAeasy Kit as per manufacturer's instruction manual. RNA samples were submitted for sequencing and bioinformatic analysis at Novogene corporation. RNA quality and quantity was assessed and served as input for poly A+ mRNA enrichment. The enriched mRNA was fragmented and reverse transcribed to cDNA, cDNA fragments were then ligated with specific adaptor sequences and used for library preparation. The cDNA library was sequenced using the Illumina NovaSeq 6000 platform.