GSM8896018: TNNAND1_N6 sequence pool, condition wo, subpopulation 5 (replicate); Saccharomyces cerevisiae; OTHER
Plasmid DNA was extracted from yeast using the Yeast Plasmid Miniprep II kit from Zymo Research. The randomized region on the plasmid was amplified via PCR and the product was purified using the Wizard SV Gel and PCR Clean-Up System from Promega. Yeast libraries were created through homologous recombination by amplifying each sequence pool with primers homologous to the linearized (AgeI+NheI) backbone pCBB06 (Boussebayle et al., 2019) and transforming S. cerevisiae RS453 with with linearized backbone and amplified pool DNA using a high efficiency electroporation protocol (Benatuil et al., 2010).