GSM9757826: Proximal petiole, replicate ID PJF15; Nicotiana benthamiana; miRNA-Seq
Total RNA was extracted using the Quick-RNA Plant Kit (Zymo Research, #R2024), generally following the manufacturer's protocol with minor adjustments based on downstream applications. For mRNA and miRNA detection, RNA was not subjected to DNAse I treatment. Small RNA libraries were made using the NEBNext Low-bias Small RNA Library Prep Kit (New England Biolabs E3420L) with NEBNext LV Unique Dual Index Primers (New England Biolabs E3400, E3402, E3404, E3406, and E3408). Amplified libraries were evaluated using an Agilent TapeStation device and D1000 Screen Tape.