GSM9431062: T5_tumor; Mus musculus; RNA-Seq
Tissues were homogenized and lysed in TRIzol reagent. After centrifuged, the supernatant (aqueous phase) was applied to the RNeasy kit (Qiagen, ID: 74106) for subsequent total RNA extraction and purification according to its protocols. RNA libraries for RNA-seq were prepared using the NEBNext® Ultra™ II Directional RNA Library kit following manufacturer's protocols.