The Probe Capture WGS Data of MTB for Sputum
Specific capture probes were designed based on 495 reference genomes covering Lineage 1-8 from NCBI and ENA databases, targeting conserved genomic regions of MTB. A total of 45 clinically suspected TB samples (including sputum, bronchoalveolar lavage fluid (BALF), and mixed samples) were collected. Three probe concentration gradients (single-sample hybridization, three-sample mixture hybridization, and six-sample mixture hybridization) were set to evaluate capture efficiency. The probe-based direct sequencing method was compared with traditional culture-based WGS in terms of sequencing quality, genome coverage, sample compatibility, and detection turnaround time. Bioinformatics analysis and paired t-test were used for performance evaluation.