Bulk-RNA_IL17a1 (CRX2182526)
RNA from mouse olfactory mucosa samples was extracted using a TRIzol reagent and quantified with a NanoDrop spectrophotometer. The RNA integrity was verified by an Agilent 2100 Bioanalyzer. mRNA was enriched using Oligo(dT) magnetic beads and fragmented into approximately 200-300 bp fragments. The cDNA libraries were constructed through first- and second-strand synthesis, end repair, A-tailing, and adapter ligation using a commercial RNA Library Prep Kit. The libraries were amplified by PCR, purified, and sequenced on an Illumina NovaSeq 6000 platform with paired-end 150 bp reads.