RNAseq after FANS of WOX2 marked cells after 3 days of somatic embryogenesis induction in Arabidopsis.
RNAseq after FANS of WOX2 marked cells after 3 days of somatic embryogenesis induction in Arabidopsis.
The upper part of the explants after 3 days of somatic embryogenesis culture was taken for nuclei isolation. Arabidopsis was grown on soil under a 16h-light/8h-dark regime at 21C Somatic embryogenesis was induced by Gaj 2001 protocol with 4,5uM of 2,4-D in the medium. RNA was extracted from FANSed nuclei with Trizol LS (Ambion). cDNA was synthesized using SMARTER technology (clontech).