GSM8895873: Retinal organoid-3, MS-VUS; Homo sapiens; RNA-Seq
Retinal organoids were dissociated into single cells using the Neurosphere Dissociation Kit (P) (Miltenyi Biotec), as per manufacturer's protocol and processed by 10X Chromium Single Cell 3' Gene Expression - 1 reaction provided to the Single Cell Analytics Facility at CMRI for portioning into single cells and library construction using the Chromium Next GEM Single cell 3' kit (10x Genomics). Library was performed accroding to the manufacturer's protocol for the 10X Genomics Chromium Single Cell 3' Gel Bead and Library kit (v3.1) at one reaction per retinal organoids single cell suspension (10,000 cells input).