GSM8996758: UAS-RFP-Rep1; Drosophila melanogaster; RNA-Seq
Total RNA was extracted by using TRIzol Reagent (Invitrogen, CAT#: 15596018) and precipitated by isopropanol. 10µg of total RNA was treated with DNase and then purified by RNA Clean & Concentrator-5 (Zymo research, CAT#: R1016). One µg of purified RNA was used for RNA-Seq library preparation by utilizing TruSeq Stranded Total RNA Library Prep kit (Illumina, CAT#: 20020596).