PacBio HiFi sequencing library of Macrocentrus cingulum: male pupae
The haploid male pupae from one host larva were selected for DNA extraction. All pupae were dissected to remove their pupal cuticles and the genomic DNA was then extracted using the AxyPrep Multisource Genomic DNA Miniprep kit (Axygen, Corning, New York, USA). After quality control, DNA was selected to construct a 15 kb SMRTbell library using the SMRTbell Express Template Prep kit 2.0 (Pacific Biosciences, Menlo Park, California, USA). The library was sequenced on PacBio Sequel II platform and the raw subreads were converted into the HiFi reads.