Inoculum - homoacetogenesis
Amplifications were carried out on a VeritiPro 96-Well Thermal Cycler (Applied Biosystems) with the following cycling conditions: initial denaturation at 95C for 3 minutes, followed by 25 cycles of 95C for 30 seconds, 55C for 30 seconds, and 72C for 5 minutes.  Successful amplification was confirmed by 1.2% agarose gel electrophoresis. Amplicon libraries were sequenced using the Illumina MiSeq platform with the MiSeq Reagent Kit v3 (300 cycles).