GSM8829413: PC3_shNC, rep 1; Homo sapiens; RNA-Seq
Total RNA was isolated from cell lines using the RNeasy Mini Kit (Qiagen). The quality of RNA was determined with the High Sensitivity RNA ScreenTape assay in TapeStation (Agilent Technologies). Libraries were prepared using NEBNext rRNA Depletion Kit v2 followed by the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) following the manufacturer's instructions. The quality of DNA libraries was evaluated using the KAPA library qualification kit (Roche) and Agilent 4200 TapeStation (Agilent Technologies).