GSM9378233: D6TAZ_TEAD4_1; Mus musculus; ChIP-Seq
Cells were subjected to cross-linking with 1.5% formaldehyde for 10 min, and nuclear extracts were subjected to ultrasonic treatment followed by immunoprecipitation overnight at 4°C with antibodies to TAZ (560235, BD Biosciences), to PPARγ (2443S, Cell Signaling), or to H3K27ac (ab4729, Abcam) or with control mouse immunoglobulin G (sc-2025, Santa Cruz Biotechnology), of which had been prebound to Dynabeads Protein A (10001D, Thermo Fisher). Antibody-bound chromatin fragments were subjected to Tn5 tagmentation with the use of TDE1 Tagment DNA Enzyme (20034197, Illumina), as described previously (Schmidl et al. Nature Methods 2015).