Flox_D7_Sample1 (CRX2131584)
Single-cell RNA libraries were constructed using the 10x Genomics Chromium Single Cell 3' Reagent Kits v3.1. Single-cell suspensions were loaded onto the Chromium Controller to form Gel Bead-in-Emulsions (GEMs) for cell barcoding and reverse transcription. Barcoded cDNA was amplified, end-repaired, A-tailed, and ligated to Illumina sequencing adapters with sample indexes. Libraries were sequenced on an Illumina platform using paired-end reads and processed with the Cell Ranger pipeline.