Oxford Nanopore whole-genome sequencing of Pharomachrus mocinno UCR5505 pectoral muscle
Genomic DNA was extracted from pectoral muscle tissue of a juvenile male Pharomachrus mocinno voucher specimen UCR5505 using the Zymo Research Quick-DNA HMW MagBead Kit. Oxford Nanopore genomic DNA libraries were prepared using the Ligation Sequencing DNA V14 kit SQK-LSK114 and sequenced on PromethION PRO-114M flow cells using PromethION 2 Solo instruments. Seven pectoral muscle sequencing runs were performed. Reads were re-basecalled using Dorado v0.9.5 with the dna_r10.4.1_e8.2_400bps_sup@v5.0.0 super-accuracy model and pooled into two FASTQ file parts for submission.