GSM8722191: WT_52x_1rep; Homo sapiens; ncRNA-Seq
Total RNA was isolated from HEK293T cells (at least ~106 cells per sample) using TRI reagent (Sigma-Aldrich) and DirectZol RNA Miniprep kit (Zymo Research, Irvine, CA, #R2050/#R2070). Small RNA-Seq (single-end strategy 50bp) libraries were prepared at Novogene Co. according to the manufacturer's instructions for the Illumina platform (Illumina, San Diego, CA).