GSM8477261: MFC7, CYFIP1 knockout 3; Homo sapiens; RNA-Seq
RNA-seq experiment and high through-put sequencing were conducted by Seqhealth Technology Co., LTD (Wuhan, China). Approximately 1×107 cells were used for RNA extraction, and DNA digestion was carried out by DNaseI after RNA extraction. The quality of RNA was determined by examining A260/A280 and 1.5% agarose gel electrophoresis. Qualified RNAs were finally quantified by Qubit3.0 with QubitTM RNA Broad Range Assay kit (Life Technologies). Total RNAs (2 mg) were used for stranded RNA sequencing library preparation, PCR products corresponding to 200-500 bps were enriched, quantified and finally sequenced on DNBSEQ-T7 sequencer (MGI Tech Co., Ltd. China) with PE150 model.