GSM8634214: Ovarian cancer/tumor/S4; Homo sapiens; RNA-Seq
Total RNA was extracted from the exosomes using TRIzol reagent (Invitrogen) following the manufacturer's protocol. RNA concentration and quality were assessed with a Nanodrop spectrophotometer and confirmed by gel electrophoresis.The RNA from anti-DHX36 RIP was extracted with TRIzol reagent according to the protocol. RNA-seq was conducted on an Illumina platform with 150-bp paired-end reads.For RIP-seq cDNA library preparation, purified RNAs were ligated to adapters, reverse transcribed, and PCR-amplified for approximately 25 cycles. The resulting libraries were then subjected to high-throughput sequencing using an Illumina Novoseq platform with a 150-nt run length.