GSM8997102: Hair cells, 1 day post sham lesion, rep 1; Danio rerio; RNA-Seq
Hair cells were isolated by fluoresence-activated cell sorting directly into lysis buffer, and total RNA was extracted using the RNeasy Plus Micro Kit (QIAGEN). 1 ng of total RNA from each sample was used for library preparation. Full length cDNA was generated using the Clontech Smart-Seq v4 Ultrat Low Input RNA kit. 1 ng of cDNA was then used to prepare libraries using the Illumina Nextera XT DNA sample preparation kit.