GSM9430481: peripheral blood, HADA, rep1; Mus musculus; ATAC-seq
Peripheral blood samples were subjected to red blood cell lysis, followed by two washes with PBS. Approximately 50,000 leukocytes were used for each ATAC-seq experiment. Cells were lysed on ice using a lysis buffer containing 10 mM Tris-HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl₂, and 0.1% IGEPAL CA-630 to release nuclei. The nuclear pellets were resuspended in a transposition reaction mix containing Tn5 transposase and incubated at 37°C for 30 minutes. Adapter 1 and Adapter 2 were added in equimolar amounts, and the libraries were amplified by PCR using the ATAC-seq_Novogene Kit