RNA-seq of Bemisia tabaci
Using total RNA as the starting sample, sequencing adapters were ligated to the 3' and 5' ends sequentially. Following reverse transcription into cDNA, the library was enriched by PCR amplification. Primer and adapter dimers were then removed by gel excision and recovery via PAGE electrophoresis. Finally, after passing quality control using Qubit and Agilent 2100, sequencing was performed on the Illumina platform in SE50 mode.