Shotgun metagenomics of soils from Trindade Island, Brazil: Trindade 61
A 450-bp library was prepared from genomic DNA using the NEBNext Fast DNA Fragmentation and Library Preparation Kit (New England Biolabs, Ipswich, NE, USA) according to the manufacturer's instructions. Library quality was verified using an Agilent 2100 Bioanalyzer, and shotgun metagenomic sequencing was performed on an Illumina HiSeq 2500 platform in paired-end mode (2150 bp).