MGISEQ-2000RS paired-end sequencing of 35 PH (Bacteria Only) fish gut metagenome (MGIEasy Universal, PCR)
Genomic DNA fragmented with Covaris ME220 (Covaris); purified and size-selected with AMPure XP beads (Beckman Coulter); library prepared with MGIEasy Universal DNA Library Prep Set (MGI) with 17-cycle PCR amplification and index barcode addition.