GSM9610860: Whole blood, CORT+DFP+LPS, 24 h, BL_24h2; Mus musculus; Bisulfite-Seq
Genomic DNA was extracted from whole blood and dissected brain tissues using standard column-based purification methods; only high-quality DNA was used for RRBS library preparation. RRBS libraries were prepared from 100 ng high-quality genomic DNA using the Ovation RRBS Methyl-Seq System (Tecan Genomics/NuGEN) according to the manufacturer's protocol, followed by bisulfite conversion and library amplification.