Ribo-Seq of Zea mays: leaves under Drought stress
Total RNA was extracted from maize (Zea mays B73) leaves using [TRIzol reagent]. Ribosome-protected fragments (RPFs) were isolated by digesting RNA with RNase I and separating the monosomes via sucrose gradient centrifugation. The resulting RPFs were purified and used for library construction with the [NEBNext Small RNA Library Prep Kit]. Briefly, the 3' adaptor was ligated, followed by reverse transcription and PCR amplification. Libraries were sequenced on an [Illumina NovaSeq 6000] platform.