PAA-TnSeq2
Tn-seq amplicon libraries were constructed from all 5 samples (1 input sample and 4 output samples) according to the protocol described in Karash et al. (2019). To check the random insertions represented in the PCR library, the fragments of Input sample was cloned in pGEM-T Easy Vector (Promega Corporation, Madison, WI, U.S.) and 20 randomly selected transformants were analyzed by Sanger sequencing.