GSM8605245: Graft animal D 1-month (ST); Sus scrofa; OTHER
For spatial transcriptomics, the grafts were collected at 1, 3 and 6 months post orthotopic transplantation in minipigs. Cells pre-bioreactor were cultured in proliferation medium in flasks, Cells post-bioreactor were cultured in proliferation medium in bioreactor in dynamic conditions for 7 days. For single-nucleus data, an aliquot of cells was collected on the day of scaffold injection and stored in freezing medium in LN2 (pre-bioreactor). Nuclei were isolated from 25um sections of recellularised scaffold and snap frozen pelleted cell mix, using Chromium Nuclei Isolation Kit (product code: 1000494, 10X Genomics, Pleasanton, CA), following 10X Genomics user guide CG000505, treating cell pellet in the same manner as the tissue.  For spatial transcriptomics data, slices of tissue were collected from OCT-embedded oesophageal samples collected post-mortem.