sample_pool_1
Splenic mRNA was harvested from healthy control JFBs and from EBOV infected bats at 3-, 7-, and 28 DPI. The mRNA was inactivated and stored in Trizol according to institutional SOP. After addition of chloroform to Trizol, RNA was extracted with Phasemaker tubes (Invitrogen). We prepared BCR libraries with the NEBNext Ultra II DNA Library Prep Kit with Sample Purification Beads (cat# E7103S) and NEBNext Multiplex Oligos for Illumina (Index Primers Set 1, cat# E7335S). Samples were cleaned using NebNext Ultra II beads and enriched for libraries with a size range of 500 to 700 base pairs amplicons, according to manufacturers instructions [71]. All libraries were sequenced using the Illumina MiSeq 2x300 chemistry platform.