GSM9892777: BPAF1; Homo sapiens; RNA-Seq
Total RNA was isolated from cell pellets using TRIzol reagent combined with RNA purification kit. RNA integrity and concentration were detected, and qualified total RNA was reserved for library construction. mRNA was enriched with oligo(dT) magnetic beads. Strand-specific paired-end mRNA-seq libraries were constructed following the manufacturer's standard kit protocol, and sequenced on Illumina NovaSeq 6000 platform.