GSM8795040: SL-KM-SI infected with lentivirus carrying  shRNA for VCAN ( TRCN0000033638), rep2; Homo sapiens; RNA-Seq
Total RNA was obtained by using Rnaeasy mini Kit (Quiagen) and following manufacturer instructions . and RNA integrity was determined by Agilent 2100 Bioanalyzer (RIN range, 9.1–9.9). Reverse stranded library preparation and RNA sequencing were conducted by BGI using the DNBSEQ platform, generating paired-end 100-bp reads. RNA isolation, converting it to cDNA, ligating adapters, amplifying, circularizing the DNA, and forming DNA nanoballs (DNBs) for high-throughput sequencing.