RNA-Seq of Homo sapiens: Healthy donor skin fibroblasts
Dermal primary fibroblast lines were expanded from skin biopsies of human subjects (ethics vote: Landesrztekammer Rhineland-Palatinate to KNW). Fibroblasts from the skin biopsies of a 57-year-old male patient with clinically confirmed Usher syndrome 2C caused by a nonsense mutation in the ADGRV1 gene (g.[90006848C>T], R2959*) were kindly provided by Dr Erwin van Wijk (Radboud University Medical Center, Nijmegen). Fibroblasts derived from the patient and from a healthy individual were maintained in DMEM/10% FBS (Thermo Fischer Scientific, 31966-021) before the RNA isolation, isolated fibroblast RNAs were subjected to RNAseq analysis with Illumina NovaSeq 6000 Sequencing Systems (Novogene) previously described (Schfer et al., 2023).