RNAseq_sgC9orf50-2 (CRX1183596)
Total RNA was extracted from MC38-sgC9orf50 and MC38-sgNTC cells using TRIzol reagent (Invitrogen), following the manufacturer's instructions. Thereafter, the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) was employed to construct the sequencing libraries. Sample clustering was performed using the TruSeq PE Cluster Kit v3-cBot-HS (Illumina), and sequencing was conducted on the Illumina NovaSeq 6000 platform.