GSM9572733: TBM, blood,  GSH011, D56; Homo sapiens; RNA-Seq
Whole blood was collected directly into PAXgene RNA tubes (QIAGEN/BD Biosciences) from 40 patients enrolled into the LASER-TBM trial. Total RNA was extracted using the PAXgene Blood RNA Kit (QIAGEN, Venlo, Netherlands). Samples were stored at -80˚C before and after RNA extraction. Quantity and quality of RNA was assessed using a Qubit fluorimeter (ThermoFisher) and Agilent 2100 Bioanalyzer. Samples with RNA integrity number (RIN) <7 were excluded. RNA libraries were constructed using Tecan's Universal Human Blood RNA-Seq kit. Ribosomal and globin RNA were removed using Tecan's AnyDeplete system. DNA library size distribution was assessed using the D1000 TapeStation kit processed on the 4200 TapeStation (Agilent Technologies). The data were analysed by Agilent's 4200 TapeStation Analysis Software. Concentration was measured using the QuantiFluor dsDNA System (Promega Corporation). Libraries were sequenced on an Illumina HiSeq4000 with a single-end 100 cycle read configuration.