18S rRNA gene sequencing of hybrid poplar soil DNA: sample JR.CM.202203.16
Library preparation and sequencing was done by the Centre dexpertise et de services, Gnome Qubec (Montral, Canada) using a nested PCR approach targeting the 18S rRNA gene of arbuscular mycorrhizal fungi (AMF), as previously described  (Stefani et al., 2020). Primers for the first PCR were AML1 5-ATC AAC TTT CGA TGG TAG GAT AGA-3 and AML2 5-GAA CCC AAA CAC TTT GGT TTC C-3 while primers for the second PCR were nu-SSU-0595-5 (5 CGGTAATTCCAGCTCCAATAG-3) and nu-SSU-0948-3 (5-TTGATTAATGAAAACATCCTTGGC-3).