WGS of Rattus norvegicus: fecal matter
The DNA was extracted from the faecal samples using the QIAGEN DNeasy Blood & Tissue Kit (QIAGEN, Germany), according to the manufacturers protocol. DNA concentration and purity were assessed using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, USA), and DNA integrity was verified by agarose gel electrophoresis prior to sequencing. Shotgun metagenomic libraries were prepared and sequenced on an Illumina NovaSeq platform with paired-end 150 bp (PE150). The minimum output and size per sample of 6.5Gb at 14 million total reads.