scRNA-Seq of rat:10 week male femur
Single-cell suspensions were prepared from rat femoral bone defect tissues (PTPG scaffolds group). The libraries were constructed using the 10x Genomics Chromium Next GEM Single Cell 3' Reagent Kits v3.1 following the manufacturer's protocol. Briefly, cells were partitioned into Gel Beads-in-emulsion for reverse transcription, and cDNA was amplified and purified. Sequencing libraries were generated by enzymatic fragmentation, end-repair, adapter ligation, and sample index PCR. The resulting libraries were sequenced on an Illumina NovaSeq 6000 platform with a paired-end configuration.