CUT&Tag of Homo sapiens: MDA-MB-231 cells, PURB antibody
CUT&Tag assay was conducted in MDA-MB-231 cells using a PURB-specific antibody. Cells were permeabilized and incubated with concanavalin A-coated magnetic beads. Primary antibody (anti-PURB) was applied, followed by a secondary antibody. pA/G-Tn5 transposase was bound to the antibody complex. Targeted DNA fragmentation was activated by addition of Mg, and released fragments were extracted and amplified by PCR. Libraries were sequenced on an Illumina NovaSeq 6000 platform with paired-end 150 bp reads.