GSM9378080: PSP2; Homo sapiens; RNA-Seq
Total RNA was extracted by using TRIzol Reagents (Invitrogen, 15596026), according to the manufacturer's protocol. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis.