GSM8605439: Sample 1 P-BCMA-ALLO1 ADT; Homo sapiens; OTHER
P-BCMA-ALLO1 lots were thawed and rested overnight in cell culture media (RPMI-1640 + 10% FBS). After rest, the cells were FACS sorted for viable cells by Live/Dead staining (ThermoFisher). Viable cells were recovered and stained by CITESeq for both protein and hashing. 3 lots were combined into 1 sample before running the cells on the 10X Chromium using the Single Cell 3' v3 kit. Libraries were constructed following the manufacturer's instructions for the 10X Single Cell 3' v3 kit CITESeq libraries for both protein and hashtag individually in addition to libraries for gene expression