TBI-ND1 (CRX2065576; CRA031363)
Poly(A) RNA from 7,000 single motor-cortex cells (recovered via the 150,000-well SCOPE-chip microfluidic workflow) was captured with oligo(dT)-coated magnetic beads, reverse-transcribed and PCR-amplified (12-14 cycles) on-chip, and the resulting full-length cDNA was used to generate scRNA-seq libraries according to the Singleron GEXSCOPE protocol.