GSM9612421: OCI-AML3 cells, shCLN7-4; Homo sapiens; RNA-Seq
Total RNA was isolated and purified using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer's procedure. RNA quantity and purity were evaluated using NanoDrop ND-1000, and RNA integrity was assessed by Agilent Bioanalyzer 2100 ensuring RIN > 7.0 Poly(A) RNA was purified from  total RNA using Dynabeads Oligo (dT) and fragmented. First-strand cDNA was synthesized using SuperScript II Reverse Transcriptase, followed by U-labeled second-strand synthesis using dUTP to generate strand-specific libraries. After A-tailing, adapter ligation, and AMPureXP bead size selection, the UDG-treated products were PCR amplified. The final libraries (average insert size 300±50 bp) were sequenced via 2x150 bp paired-end sequencing on an Illumina NovaSeq 6000 platform.