8k MP WGS library of Streptomyces bambergiensis VKPM AC-800
gDNA was used to create a sequencing library using the Nextera Mate Pair Library Preparation Kit (Illumina) according to the manufacturer's instructions, aiming for an insert size of 8k. Sequencing was done on an Illumina MiSeq platform using a MiSeq Reagent Kit v3 in a 2x 300nt run. The paired reads were joined using flash with parameter -M 250. After joining, the reads were split on the adapter sequence with "cutadapt -a CTGTCTCTTATACACATCT --trimmed-only -m 20" for the forward direction and "cutadapt -g AGATGTGTATAAGAGACAG --trimmed-only -m 20 | sed "s/ 1:N/ 2:N/" | seqtk seq -r" for the reverse direction.