Genomic Diversity and Climate-Driven Habitat Shifts of S. moorcroftiana_BL_10 (CRX2039286)
Genomic DNA was extracted from silica-dried seed tissues using the cetyltrimethylammonium bromide (CTAB) method. DNA quality was assessed using a Qubit fluorometer (Invitrogen, USA) and NanoDrop spectrophotometer (Thermo Scientific, USA). Qualified DNA samples (concentration > 1 ng/uL and total DNA > 0.05 ug) were used for paired-end sequencing (150 bp and insert size of 450 bp) on the DNBSEQ-T7 platform.