Nor1_3 (CRX2058486; CRA031131)
mRNA was enriched by binding magnetic beads with Oligo (dT) to the polyA tail of mRNA via A-T complementary pairing. Subsequently, mRNA was fragmented into approximately 300-bp fragments using a fragmentation buffer. Following reverse transcription into cDNA and ligation with adapters, the fragmented products were purified, sorted, and amplified by PCR. The resulting library was sequenced using a NovaSeq 6000 sequencer