GSM9078739: PBMCESO_LN; Homo sapiens; RNA-Seq
Lung (the tumor site) and periphery blood cells harvested from experimental animals at Day 21 were subjected to FACS sorting for isolating Allo15ESO-T cells (hCD45+TCRVB13.1+hCD3+; Sample_02, Sample_03), and PBMCESO-T cells (hCD45+TCRVB13.1+hCD3+; Sample_05, Sample_06). Cells isolated from 6 experimental mice were combined for each group. Preinfusion sampling of therapeutical cells (Sample_01: Allo15ESO-T, Sample_04: PBMCESO-T) were also sorted and subjected to scRNA-seq. Cells were quantified using a Cell Countess II automated cell counter (Invitrogen/Thermo Fisher Scientific). A total of 10,000 cells from each experimental group were loaded on the Chromium platform (10X Genomics), and libraries were constructed using the Chromium Next GEM Single Cell 3' Kit v3.1 and the Chromium Next GEM Chip G Single Cell Kit (10X Genomics), according to the manufacturer's instructions. Library quality was assessed using the D1000 ScreenTape on a 4200 TapeStation System (Agilent Technologies). Libraries were sequenced on an Illumina NovaSeq using the NovaSeq S4 Reagent Kit (100 cycles; Illumina).