Hi-C sequencing of D7
Fresh leaves were cut into 2-3 mm pieces and fixed with formaldehyde to covalently link chromosomal loci through protein-DNA interactions. Tissues were then lysed to extract nuclear DNA for being digested with MboI (NEB, R0147M). The sticky ends were marked with biotin-14-dCTP during the end repair process, and proximity-ligated to form chimeric junctions. Subsequently, the protein at the junctions was digested to release DNA from the protein-DNA cross-linking complexes. The DNA was extracted and randomly sheared into fragments of ~ 350 bp. Next, paired-end sequencing libraries were prepared based on the biotin-labeled and ligated DNA fragments, and subsequently sequenced on Illumina NovaSeq 6000 following the manufacturers instructions.