GSM9610124: pWPI_control_Biol_rep_2; Homo sapiens; RNA-Seq
Total RNA was isolated 48 hours post-transfection using TRIzol Reagent (Thermo Fisher Scientific) according to the manufacturer's protocol. RNA concentration and purity were determined using a NanoDrop spectrophotometer, and RNA integrity was confirmed before library preparation. Libraries were prepared using the TruSeq Stranded mRNA Library Prep Kit (Illumina) following the manufacturer's protocol (Reference Guide #1000000040498). The process included poly-A selection of mRNA, followed by fragmentation, cDNA synthesis, adapter ligation, and PCR amplification. The resulting libraries were sequenced on an Illumina platform.