Teratoscincus roborowskii Genome sequencing and assembly
Genomic DNA was extracted and sequenced for small fragment library construction. It is divided into the following four steps:(1) Library construction: the qualified genomic DNA was fragmented to the target fragment (350 bp) by physical fragmentation method (ultrasonic shock), and then the small fragment sequencing library library was constructed after end repair, A addition, splice addition, target fragment selection and PCR;(2) Library QC: Qseq400 and Qubit were utilized to detect the fragment size and library quantification to determine whether the library meets the sequencing standards;(3) Chip immobilization: immobilize the library onto the sequencing chip by bridge PCR;(4) Sequencing: the library was sequenced by double-end 150 bp (PE 150) sequencing using Illumina sequencer, and the data generated by sequencing was used for the next step of information analysis after quality control.