K85 (CRX2066064; CRA031378)
Bacterial 16S rRNA V3-V4 regions were amplified using barcoded primers 338F/806R via 25-cycle PCR (98 deg C denaturation/53 deg C annealing/72 deg C extension, Fast pfu polymerase), purified with Vazyme beads, PicoGreen-quantified, equally pooled, and sequenced on Illumina NovaSeq (2x250 bp)