RNA-Seq of Gallus gallus: early postmitotic neurons
RNA-seq libraries were prepared from total RNA (200ng) using the NEBNext Ultra II RNA Sample Preparation Kit for Illumina (New England BioLabs, Ipswich, MA) according to the manufacturer's protocol. Sequencing runs were executed on the Illumina NextSeq500 instrument (Illumina) using single-end 75-cycle sequencing. Raw sequence data (.bcl files) was converted into fastq files and demultiplexed using Illuminas bcl2fastq software.