GSM9537943: miR_PBS_1, miR146a KO pancreas, PBS, replicate 1; Mus musculus; RNA-Seq
Total RNA was isolated from snap-frozen mouse pancreatic tissues using TRIzol (Sigma). Libraries were generated using stranded polyA libraries prepared using Nugen Universal Plus mRNA-seq (Part No. 0508), protocol M01442 v2 at South Australian Genomics Centre (SAGC), Adelaide, Australia. PE100 sequencing (R1 – 98; R2 – 98; Barcode – 16; Dual Barcode – 8) was done using MGI DNBSEQ G400 FCL PE100 chemistry..