GSM8513171: Equal amount of DMSO incubated with RNA extract from E. Coli - copy 3; Escherichia coli; RNA-Seq
RNA was isolated after biotin-streptavidin bead pulldown using 50 mM DTT. Eluted RNA samples were cleaned up using RNA Clean and ConcentratorTM-5 (Zymo) and dissolved in nuclease-free water. 25 ng of RNA was used per sample for construction of sequencing libraries. RNA libraries for RNA-seq were prepared using the KAPA RNA Hyperprep kit following manufacturer's protocols