DNA-Seq of Curtobacterium flaccumfaciens pv. flaccumfaciens
The Illumina DNA Prep, (M) Tagmentation kit was used according to the manufacturers specifications to generate 150PE Illumina-ready libraries. Illumina Unique Dual Indexes were used for indexing. A total of five amplification cycles were selected during the library generation step. Library yields were quality assessed by fluorescence on a Qubit 4.0 fluorometer (Thermo Fisher Scientific, MA, USA ), and correct insert sizes were verified using D1000 ScreenTape on a TapeStation 4200 system (Agilent Technologies, CA, USA). Libraries were equimolarly pooled targeting at least 2 Gb per sample, and 150PE sequencing was performed using a 300-cycle P1 cartridge and flow cell combination on a NextSeq 2000 system (Illumina, CA, USA).