RNA-seq transcriptomics of doxorubicin (DOX) exposure in mouse colon tissue (in vivo; 0, 5, 10 mg/kg; 6, 24, 72, 96 h), with raw paired-end FASTQ files.
RNA-seq transcriptomics of doxorubicin (DOX) exposure in mouse colon tissue (in vivo; 0, 5, 10 mg/kg; 6, 24, 72, 96 h), with raw paired-end FASTQ files.
Male C57BL/6J mice (10 weeks old) received doxorubicin (5 or 10 mg/kg) by i.v. bolus injection on days 0 and 1 and were euthanized at 6, 24, 72, or 96 h after treatment initiation (Gall et al. 2023). Colon mucosa was collected for transcriptomic profiling following reported procedures; vehicle controls were handled in parallel. Total RNA was extracted using the miRNeasy Mini Kit (Qiagen). Total RNA was extracted using the miRNeasy Mini Kit (Qiagen) following the manufacturer's instructions. RNA quantity/purity were assessed by NanoDrop and RNA integrity was assessed by Agilent 2100 Bioanalyzer; samples with RIN > 6 and sufficient yield were used for library preparation. mRNA sequencing libraries were prepared from total RNA using the Lexogen SENSE mRNA-seq library preparation kit, following the manufacturer's instructions, including sample indexing for multiplexing.