GSM8635050: dataset1_stages_rep3; Danio rerio; RNA-Seq
Fins were choped to small pieces and isolated with liberase. Digestion was stopped by adding FBS and lysate was filtered and washed with 1xPBS before loading into chips. Cells were captured in a Chromium controller (10X Genomics). Single-cell mRNA libraries were prepared using the single-cell 3' solution V3 kit (10X Genomics). Quality control and quantification assays were performed using a Qubit fluorometer (ThermoFisher) and a 2100 Bioanalyzer (Agilent). Libraries were sequenced on an Illumina NextSeq 550 using 75-cycle, high output kits (read 1: 26 cycles, i7 Index: 8 cycles, read 2: 57 cycles).