XQ_infl_ONT (CRX2091911)
RNAs were transcribed into cDNAs, and then full-length cDNAs were enriched following cDNA PCR for 14 circles with LongAmp Tag (New England Biolabs (Beijing) LTD). Defined adaptors were ligated to the ends of PCR products using T4 DNA ligase (NEB). The final cDNA libraries were subjected to high-throughput sequencing on a PromethION platform of Oxford Nanopore Technologies (ONT).