GSM9756809: Vehicle 2; Mus musculus; RNA-Seq
RNA was “harvested” by sorting cells directly into lysis buffer: live GP33-specific (GP33⁺) CD8⁺ T cells were FACS-sorted (500–1,000 cells/well) into a 96‑well plate containing cell lysis buffer. Lysates were stored: the plate was stored at −80 °C. RNA libraries for RNA-seq were prepared using the SMART-seq HT Ultra Low input protocol