RNAseq of Selasphorus rufus: Iridescent green feather
Total RNA for RNAseq was extracted from homogenized pin feathers with a New England Biolabs Monarch Total RNA miniprep kit. Quality of extracted RNA (RINe) was verified with an Agilent Technologies 2200 Tapestation and all samples had a of RINe 8.2 or greater. Samples were sent to Azenta Life Sciences in South Plainfield, New Jersey for mRNA purification through Poly(A) selection and paired-end sequencing on an Illumina NovaSeq.