GSM9609970: salmonella exposed to tryptophan 10 minutes rep 2; Salmonella enterica; RNA-Seq
Total RNA purification was performed using Norgen kits for the rapid purification of total RNA, including microRNA without the use of phenol (Norgen Biotek, Cat no. SKU 17200). Chemical and mechanical lysis was performed using 1mg/ml of lysozyme and vertexing as described in section 1E of the kit protocol, suitable for gram negative bacteria. Avraham, R., Haseley, N., Fan, A., Bloom-Ackermann, Z., Livny, J., Hung, D.T., 2016. A highly multiplexed and sensitive RNA-seq protocol for simultaneous analysis of host and pathogen transcriptomes. Nat. Protoc. 11, 1477–1491. https://doi.org/10.1038/nprot.2016.090