TU3DPEP3.HAR
A total of thirteen Vibrio strains were selected for whole-genome sequencing. These strains represented the most frequently isolated Vibrio species and, when possible, included isolates from both reef sites and healthy and diseased coral tissues. Glycerol stocks of the selected strains were cultured in the original isolation media. Bacterial cells were then pelleted by centrifugation, and high-molecular-weight DNA was extracted using the Monarch HMW DNA Extraction Kit (New England Biolabs, Ipswich, MA). Sequencing libraries were prepared using the Ligation Sequencing Kit and the Native Barcoding Kit 24 from Oxford Nanopore Technologies (Oxfordshire, UK). Sequencing was performed on an R10 Nanopore flow cell (FLO-MIN114) paired with kit 14 chemistry on a MinION device over a 48-hour run time. The raw signal data (fast5 files) were base-called using Guppy basecaller version 2.2.3 (Oxford Nanopore Technologies) with the super-accuracy base-calling model in MinKNOW (version: 24.02.16.).