Rhodotorula glutinis of batch fermentation at day 5
Sample collected on day 5 from Rhodotorula glutinis monoculture fermentation. Yeast cells were cultivated under nitrogen-limited conditions using high-concentration acetate as the sole carbon source. RNA-Seq was performed to investigate gene expression dynamics during fermentation in Yarrowia lipolytica monoculture, Rhodotorula glutinis monoculture, and a mixed co-culture (YL:RG = 7:3). Strand-specific mRNA libraries were prepared using the Illumina Stranded mRNA Prep with Ligation protocol and sequenced on an Illumina NovaSeq X Plus platform.