Metagenomic sequencing of mouse feces
Shotgun metagenomic sequencing of fecal microbiota from mice. Fecal samples were collected from mice, and total genomic DNA was extracted. DNA libraries were prepared using a standard whole-genome shotgun library preparation protocol with ~350 bp insert size, and paired-end sequencing (2  150 bp) was performed on an Illumina NovaSeq 6000 platform. Raw sequencing data were used for metagenomic analysis including taxonomic profiling, functional annotation, and comparative analysis of gut microbial communities.