GSM8829285: mTNBC patient 2_Case; Homo sapiens; RNA-Seq
Peripheral blood mononuclear cells (PBMC) of female mTNBC were obtained from a clinical study approved by the institutional review board of the Korea University Medical Center Anam Hospital, Seoul, the Republic of Korea (no. ED17183). PBMCs of two healthy donors with no known medical history of cancer or other general conditions such as hypertension, diabetes, hyperlipidemia, and cardiovascular and inflammatory diseases were collected for comparison. In brief, whole blood samples were collected, and PBMCs were isolated by Ficoll–Paque™ density-gradient centrifugation. ScRNA-seq libraries were prepared using the Chromium Single Cell 3′ v3.1 platform (10X Genomics) according to the manufacturer's protocol. In brief, 10,000 cells were loaded into each channel of the Chromium X controller to achieve the target cell recovery of 5,000 cells. The cells were partitioned into gel bead emulsions, where cell lysis and barcoded reverse transcription of RNA were performed, followed by cDNA amplification. The amplified cDNA was purified using SPRI beads, and dual-indexed libraries were constructed by fragmentation, end repair, A-tailing, adapter ligation, and PCR amplification.