Epidermis, Mice, Control DMBA Sample 2
For whole genome sequencing (WGS), frozen epidermal samples were then minced and homogenized in lysis buffer and DNA was isolated using QIAamp DNA Mini Kit (Qiagen; Cat#56304) according to manufacturer protocol. For WGS library preparation, DNA concentration, quality and integrity of DNA was checked using Qubit and 5200 Fragment Analyzer. DNA was fragmented using Covaris and libraries were prepared using Swift 2S Sonic DNA Library kit (Swift Biosciences; Cat# 42024) kit according to manufacturer protocol. After purifications, quality of the libraries was assessed on 5200 Fragment Analyzer with DNA Chip and libraries were sequenced and analyzed by MedGenome Inc (Foster City, CA).