GSM8605731: Male term non-diabetic (T-ND), RNAseq, rep 1; Mus musculus; RNA-Seq
One kidney was stored in RNAlater™ Stabilization Solution (Sigma Aldrich, St. Louis, MO). RNA extraction and cleaning were performed using TRIZOL (Invitrogen, Waltham, MA) and RNeasy MinElute Cleanup Kit with RNase-Free DNase (QIAGEN, Hilden, Germany). Extracted RNA was reconstituted with RNase-free water to a final volume of 50 µl and stored at -80°C until analysis. RNA concentration and OD260/280 were measured with a NanoDrop Spectrophotometer (Thermo Fisher, Waltham, MA). Whole kidney RNA-Seq was performed by Novogene, and only samples with high purity (OD260/280>2.0; RIN>4.0) were analyzed. Whole kidney RNA-Seq was performed by Novogene, and only samples with high purity (OD260/280>2.0; RIN>4.0) were analyzed.