GSM9139424: E150009242_L01_T2; Mus musculus; RNA-Seq
centrifuge to remove supernatant, add about 2ML PBS (2% FBS), with a total amount of about 2.5mL, Add cells to trizol RNA was harvested using Rneasy mini plus kit (Qiagen). 1.3ug of total RNA was used for the construction of sequencing libraries.RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.