GSM8511784: IN_6_P; Danio rerio; RNA-Seq
For each sample, 10-12 neurons were collected and transferred to lysis buffer solution. Total RNA was extracted for construction of cDNA libraries, involving fragmentation, PCR enrichment, N7 index linking, and purification. A total of 71 QuantSeq libraries (N=9-12 samples for each subpopulation) were prepared using the Lexogen's QuantSeq 3′ mRNA-Seq Library Prep Kit for Illumina according to the manufacturer's instructions.