GSM8513212: 3mo313, Pancreas, WT; Mus musculus; RNA-Seq
Tumor samples were collected in RNA later and digested in a tissue homogeniser (Fastprep FP120 cell disruption system). RNA was harvested using Rneasy mini plus kit (Qiagen). 500ng of total RNA was used for the construction of sequencing libraries. RNA librarier for RNA-seq were prepared using TrueSeq stranded mRNA library Prep according to manufacturer's protocol (Illumina).