RNA-Seq of Homo sapiens: FADS2-knockdown BPH-1 cells
A total of 1 g of RNA per sample was used to construct sequencing libraries with the NEBNext UltraTM RNA Library Prep Kit, including mRNA purification, fragmentation, cDNA synthesis, adapter ligation, size selection (250-300 bp), USER enzyme treatment, PCR amplification, purification, and quality assessment on the Agilent Bioanalyzer 2100 system.