GSM8995944: Ctrl; Mus musculus; OTHER
C3H 10T1/2 were harvested by 0.05% trypsin. 10000 cells per sample were used as starting material. Libraries were amplified using 13 cycles on the thermocycler. Post amplificationlibraries were size selected at 250-450bp in length using Agencourt AMPure XP beads fromBeckman Coulter. Cut&Tag libraries were prepared using Hyperactive Universal CUT&Tag Assay Kit for Illumina (Vazyme, TD903). DNA was subjected to PCR amplification by universal barcoded i5 and i7 primers from TruePrep Index Kit V2 for Illumina (Vazyme, TD202) with 14 cycles on the thermocycler.