Multiome sequencing of human meninges development
Multiome sequencing of human meninges development
Samples were collected from embryos obtained under ethical approvals in place, from terminations of pregnancy. Samples were stored in hypothermosol and micro dissected under a Leica microscope, kept at 4 degrees celsius. Following 10x genomics multiome droplet capture, cDNA libraries were generated, according to manufacturer protocols, for 10x genomics multiome kit. Quality control of cDNA and final libraries was done using Bioanalyzer High Sensitivity DNA Analysis (Agilent).