GSM8722540: BRB sequencing from gastruloids grown from different cell seeding numbers; Mus musculus; OTHER
For each replicate (three in total), 60 gastruloids from each condition were collected in a 1.7ml Eppendorf tube and washed once with PBS, pelleted and kept in -80°C until all samples were collected. Each sample were then thawed and extracted using the RNeasy mini kit (Qiagen) according to manufacturer's recommendation with on-column DNase digestion. Libraries were performed using Alithea Genomics Mercurius protocol v.0.2.2 and sequenced on Novaseq 6000 in a PE run with 28, 8i, 90 configuration.