Proximo Hi-C Sequencing of Spirinchus thaleichthys: Single-Sample Hi-C
Proximo Hi-C sequencing performed by Phase Genomics. Following the manufacturer's instructions for the kit, intact cells from two samples were crosslinked using a formaldehyde solution, digested using the DPNII, DDE1, HINF, and MSEI restriction enzymes, end repaired with biotinylated nucleotides, and proximity ligated to create chimeric molecules composed of fragments from different regions of the genome that were physically proximal in vivo, but not necessarily genomically proximal. Continuing with the manufacturer's protocol, molecules were pulled down with streptavidin beads and processed into an Illumina-compatible sequencing library.