16S rRNA gene sequencing of human gut metagenome: feces
16S rRNA gene sequencing targeting the V3-V4 hypervariable region using the primers 341F (5-CCTACGGGNGGCWGCAG-3) and 785R (5-GACTACHVGGGTATCTAATCC-3). Genomic DNA was extracted from 500 mg fecal aliquots using the QIAamp Fast DNA Stool Mini Kit after homogenization (Precellys 24). Libraries were prepared using the standard 2-step PCR Illumina protocol with Nextera XT indices. Final libraries were pooled equimolarly and sequenced on an Illumina MiSeq platform using a 2x300 bp paired-end protocol.