AC-2 (CRX2057832; CRA031108)
Total RNA was extracted from primary bone marrow mesenchymal stem cells (BMMSCs) using an RNA extraction kit. After passing quality control, RNA with a Poly-A structure in total eukaryotic RNA was enriched using the TIANSeq mRNA Capture Kit (TIANGEN Biotech). Subsequently, the captured RNA was used as the starting sample for library construction, and library preparation was conducted using the TIANSeq Rapid RNA Library Prep Kit (Illumina Platform) (TIANGEN Biotech). The construction of the transcriptome sequencing library was completed through steps including RNA fragmentation, first-strand/second-strand cDNA synthesis, end repair, A-tailing, adapter ligation, and PCR library enrichment.