GSM8656119: input_2; Homo sapiens; ChIP-Seq
Cross-linked protein-DNA complexes were enriched with a PML-specific antibody, de-crosslinked, treated with RNase A and Proteinase K and purified using a QIAquick PCR purification kit (Qiagen, Cat#28106). Libraries were prepared at Novogene Bioinformatics Technology Co. Ltd using the NEBNext Ultra II DNA Library Prep Kit and sequenced as paired-end 150bp reads on an Illumina NovaSeq 6000 sequencer.