GSM9108540: MD901 vehicle_rep3; Homo sapiens; ChIP-Seq
At day 5 cells were fixed with 1% formaldehyde for 10min and sonicated using the S220 Focused-ultrasonicator (Covaris) for 15min. Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated after immunoprecipitation with the H3K27ac antibody (Abcam Cat# ab4729) using Protein A and Protein G Dyna beads (Invitrogen). Samples were washed prior to overnight reverse crosslinking.Chromatin was isolated with the ChIP DNA Clean and concentrator (Zymo Research) . Libraries were prepared using the DNA Library Prep NEB (New England Biolabs)