GSM8605375: hippocampus microglia, WT, 1613; Mus musculus; RNA-Seq
Total RNA was extracted from sorted microglia of hippocampus of P17-21 mice using a TRIzol reagent (Thermo Fisher). mRNA was reverse transcribed and amplified using the SMART-seq method. RNA-seq libraries for were prepared using TruePrep DNA Library Prep Kit V2 for Illumina following manufacturer's protocols.