Duck transcriptome sequencing data
The total RNA was incubated with oligo dT magnetic beads, and then the mRNA was eluted from the magnetic beads with an elution buffer, thus obtaining purified mRNA. The mRNA was synthesized into cDNA using reverse transcriptase. The cDNA was subjected to end repair and addition of A-tail treatment, and then sequencing adapters were ligated to both ends of the cDNA fragments. The cDNA fragments with adapters were enriched by PCR amplification, and a transcriptome sequencing library was constructed. The libraries that passed the quality inspection were sequenced on a sequencing instrument (Illumina sequencing platform).