WGS pantoea agglomerans
Genomic DNA isolation was completed from two duplicate cultures grown in LB media using the Monarch Genomic DNA Purification Kit (#T3010S) using the Gram-negative extraction protocol. Library preparation was performed using the Oxford Nanopore Technologies Rapid PCR Barcoding Kit 24 V14 (SQK-RPB114.24). Protocol was followed using the manufacturers protocol except for the number of PCR cycles which was set to 25 cycles. Sequencing was performed with Oxford Nanopore Technologies MinION Flow Cell R10 Version (FLO-MIN114)