The gut vagal sensory pathway drives postprandial sleep via activation of PVH-projecting GABAergic neurons in the NTS (CRX2044901)
The concentration of the single-nucleus suspension was adjusted to 3-4 x 10^5 nuclei/mL in PBS before loading onto a microfluidic chip (Singleron Biotechnologies). SnRNA-seq libraries were then constructed using the GEXSCOPE Single-Nucleus RNA-seq Kit (Singleron Biotechnologies) according to the manufacturer's instructions. The libraries were sequenced on an Illumina NovaSeq 6000 platform with 150 bp paired-end reads.