GSM9645162: RNA from tonsil, donor 1; Homo sapiens; RNA-Seq
RNA extracted from memory B cells obtained by magnetic enrichment from a human tonsil For classical RNA-seq libraries, 1200 ng of total RNA (with RIN over 7) were used for polyA selection and reverse transcription using the Truseq stranded mRNA library kit (Illumina). Sequencing (2x150 bp paired ends) was run on a Novaseq Illumina platform (Macrogen) in order to acquire 15 Gb of throughput per sample.