Nanopore WGS of Pseudomonas oleovorans ERA
Whole genomic DNA was extracted from an overnight sample grown in LC media and initiated from a single colony. Oxford Nanopore sequencing was performed by Plasmidsaurus. Library selection was limited to 0.6x bead cleanup to remove highly degraded fragments <450bp. An amplification-free long-read sequencing library was prepared using v14 library prep chemistry and sequenced using R10.4.1 flow cells.