fourth female rat section 1
Liver tissue of 86 day old Lewis rats was included. Each liver was sectioned into three sections. RNA was isolated using the NucleoSpin RNA kit by Macherey Nagel. RNA quality was assess via NanoDrop 2000, Agilent Bioanalyzer, Qubit and RNA bleach gel. Library preparation was performed with QuantSeq 3 mRNA-Seq Library Prep kit FWD (Lexogen). RNA sequencing was performed on a NextSeq2000 (Illumina).