WGS of adult Canis lupus familiaris
Total 100ng DNA samples. Used QIAseq FX DNA Library Kits and QIAseq UDI Y-Adapter Kit C. The fragmented DNA was directly end-repaired and an A was added to the 3 ends during the FX reaction, making the DNA fragments ready for adapter ligation. Following this step, platform-specific adapters were ligated to both ends of the DNA fragments. These adapters contain sequences essential for binding dual-barcoded libraries to a flow cell for sequencing, allowing for PCR (6 cycles) amplification of adapter-ligated fragments, and binding standard Illumina sequencing primers. Following library construction, the reaction cleanup and removal of adapter-dimers was achieved by using QIAseq Beads.