Genome sequencing approach to gene discovery in loose anagen hair syndrome
Massively parallel genome sequencing (GS) was performed using the NovaSeq6000 plat-form (Illumina, San Diego, CA, USA) and KAPA Hyper Prep Kit (Roche, Indianapolis, IN, USA) for 300 cycles at the Iowa Institute of Human Genetics (Iowa City, IA, USA). The paired-end reads were analyzed using the DRAGEN platform for read alignment, and variant calling (Illumina, San Diego, CA, USA).