GSM8794338: myotubes, Nrip1KO, IgG, rep1; Mus musculus; OTHER
Nuclei were isolated from myotubes utilizing the nuclei extraction protocol (CUT&TAG Kit Version 1 by EpiCypher) and proceeded to the CUTANA™ ChIC/CUT&RUN Kit Version 4 protocol (EpiCypher, New England Biolabs) following manufacturer's instructions. CUT&RUN libraries were constructed using CUTANA™ CUT&RUN Library Prep Kit (EpiCypher, New England Biolabs). 5 ng of DNA was utilized for library construction. To barcode samples, unique combinations of i5 and i7 indexing primers were used to amplify during PCR.