GSM8460903: iCKO_rep3; Mus musculus; RNA-Seq
Total RNA was extracted using a RNAsimple Total RNA Kit (DP419, TIANGEN)；genomic DNA was extracted with nuclei dilution buffer (20 mM Tris Cl pH 8.0, 2 mM EDTA, 150 mM NaCl, 1% NP40, 1% SDS, and 1×PI). For total RNA-seq, the libraries were constructed with TIANSeq Fast RNA Library Prep Kit (NR102, TIANGEN). Total RNA was extracted using a RNAsimple Total RNA Kit (DP419, TIANGEN)；genomic DNA was extracted with nuclei dilution buffer (20 mM Tris Cl pH 8.0, 2 mM EDTA, 150 mM NaCl, 1% NP40, 1% SDS, and 1×PI).