Ocean metagenomes from the 2023 UNOLS AICC Chief Scientist Training Cruise.
Seawater was prefiltered with 100-m Nitex mesh and then microbial cells were collected on 0.1 m filters. Following DNA extraction, DNA was sheared to ~200-400 bp with a Covaris, libraries were prepared using the KAPA HyperPrep, and libraries were paired-end sequenced on an Illumina NovaSeq 6000.