GSM9432944: iKCs, SAM mut, biol rep 1; Homo sapiens; RNA-Seq
cells were sorted in 300 nL lysis buffer overlayed with 3 μL Silicon Oil (5 cSt; Sigma-Aldrich) using a CytoFlex SRT sorter (100 μm nozzle; Beckmann Coulter). reverse transcription was carried out by adding 100 nL reverse transcription master mix. Preamplification of cDNA was performed in 1 μl total volume using SeqAmp polymerase (Takara Bio #) for 14 cycles. Subsequently, amplified cDNA was diluted with 9 μl H2O and we used 1 μl for downstream tagmentation using 0.004 μl TDE1 enzyme per cell. Final, indexed libraries were pooled per 384-well plate, cleaned and equimolarly pooled before sequencing