Hi-C of Lwui: adult female liver
Around 1 g of the liver tissue was fixed with 1% formaldehyde to coagulate the conformation of DNA. The gDNA was then enzymatically digested with MboI (a 4-cutter restriction enzyme), generating fragments with sticky ends. The restriction fragment overhangs were repaired, and biotin was introduced to label the ends of the oligonucleotides. Subsequently, the DNA fragments were ligated together to form chimeric circles using DNA ligase. These circles were then uncrosslinked, purified, and sheared into fragments ranging from 300 to 500 bp. Finally, the biotinylated fragments were captured using streptavidin-coated beads.