GSM9433341: Multiplexed, human PBMC, naive CD4+ T cells, GEX; Homo sapiens; OTHER
PBMCs were collected from healthy adult female donors [IRB protocol, STUDY02000116: VISTA in Autoimmune Disease to Christopher Burns]. Human PBMC were pre-enriched directly from total blood with BioLegend EasySep™ Direct Human CD4+ T Cell Isolation Kits. Human cells were sorted as singlet, viability dye-, CD4+, CD8-, CD45RA+, CD45RO-, CD127hi, CD25-, CD95-. Droplet-based 5′ end scRNA-seq was performed using the 10x Genomics platform and libraries were prepared and sequenced on a NextSeq2000 using 28×90bp reads for GEX libraries using the Chromium Single Cell NextGEM 5' v2 chemistry Reagent kit.