GSM9645339: High-P-C; Parasynechococcus marenigrum WH 8102; RNA-Seq
Filters containing Synechococcus sp. WH8102 cells (~700 mL culture per sample) were processed using TRIzol reagent. Cells were lysed with lysozyme and mechanically disrupted with 0.1 mm beads. RNA was extracted via phase separation with chloroform, precipitated with isopropanol, washed with 70% ethanol, and resuspended in nuclease-free water. Residual genomic DNA was removed by TURBO DNase treatment, and RNA was further purified with phenol-chloroform. RNA integrity and concentration were assessed using Nanodrop and Qubit instruments. RNA libraries were prepared from ribosomal RNA-depleted RNA using standard protocols and sequenced by SeqCoast Genomics. Libraries were sequenced as paired-end 2 × 150 bp reads, generating approximately 12 million reads per sample on an Illumina platform.