Depth Profile Eukaryotic Metatranscriptomes from the North Pacific Ocean
Total RNA was extracted using the Direct-zol RNA MiniPrep Plus kit (Zymo Research R2702), quantified using a Qubit fluorometer 1.0 (Thermofisher) and quality controlled using a Bioanalyzer 2100 (Agilent) prior to sequencing. Samples were randomized across sequencing runs to reduce potential biases. Poly(A)-selected eukaryotic mRNAs were sequenced on the Illumina NovaSeq 6000 platform using the S2 flow cell with 300 cycles. Library prep with poly(A)-selection and sequencing (150bp paired end reads) was performed at the Northwest Genomic Center (University of Washington, Seattle).