Whole Shotgun metagenomic sequencing of 4317 Ptrap biofilm
Genomic DNA was extracted using the DNeasy PowerSoil Kit (Qiagen) following the manufacturers protocol with modifications to enhance yield. DNA was eluted in 50 l of C6 buffer. DNA concentration and quality were assessed using a Qubit 4 Fluorometer with the 1X dsDNA HS Assay (Invitrogen). Extracted gDNA was stored at 20C until shipment at ambient temperature. Extracts were paired-end sequenced up to a read length of 150bp on Illumina NovaSeq platform, using a commercial provider (Azenta GeneWiz, UK).