SC6-6-F3-3-WGS (CRX898010)
The extracted DNA was initially randomly sheared using ultrasonication. Subsequently, the DNA sequence's ends were repaired by using the End Repair Mix2 in the kit, removing overhanging bases from the 5' end, adding a phosphate group, and filling in missing bases from the 3' end.An A base was added to the 3' end of the DNA sequence to prevent self-ligation of DNA fragments while ensuring the target sequence could ligate with the sequencing adapter (which has a protruding T base at its 3' end). A sequencing adapter containing a library-specific label (i.e., Index sequence) was added to the 5' end of the sequence, allowing DNA molecules to be fixed on the Flow Cell.BECKMAN AMPure XP Beads were employed to selectively remove self-ligated adapter fragments and purify the library system after adapter addition.The DNA fragments with attached adapters underwent PCR amplification to enrich the sequencing library template. The resulting library enrichment product was purified once again using BECKMAN AMPure XP Beads.The library underwent final fragment selection and purification using 2% agarose gel electrophoresis. The library insert fragments were approximately 450 bp.