16s rRNA metagenomic sequencing of Polymicrobial biofilm
Bacterial genomic DNA was extracted from multi-species biofilms of F. necrophorum, B. pyogenes, and P. levii using the DNeasy Blood & Tissue Kit (Qiagen) following the manufacturers protocols. A sequencing library targeting the V3-V4 region of the 16S rRNA gene was constructed and sequenced on the Illumina MiSeq platform using a 2250 bp paired-end configuration.