GSM8634701: PE001; Homo sapiens; RNA-Seq
After collecting placental tissues, they were immediately processed into single-cell suspensions. The process involved washing, cutting, digesting, filtering, lysing red blood cells, removing dead cells, staining, and finally suspending the cells in a solution. The single-cell suspension was loaded onto the Chromium Controller to generate single-cell gel beads, followed by single-cell RNA sequencing using the Illumina Novaseq6000 sequencer. The single-cell suspension was loaded onto the Chromium Controller according to the manufacturer's instructions (10X Genomics) to generate single-cell gel beads. After the generation of single-cell gel beads, single-cell RNA-seq libraries were constructed using the single-cell 3' library and gel bead kit V3.1 on an Illumina Novaseq6000 sequencer, employing a paired-end strategy with 150 bp read lengths, to generate sequencing data of at least 150 gigabases per sample.