RNA-Seq of Salmo salar adductor mandibulae
For RNA extraction, mRNA isolation, RNA-seq library preparation, and sequencing, 72 samples were shipped on dry ice to Novogenes Cambridge Sequencing Centre (Cambridge, UK). To prepare sequencing libraries, messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis 37. Following end repair, A-tailing, adapter ligation, size selection, amplification, and purification, libraries were checked with Qubit and real-time PCR for size distribution. Quantified libraries were then pooled and sequenced on the Illumina NovaSeq X Plus (PE 150) platform to generate approximately 12G raw data per sample.