RNA-Seq of WT and Mecp2 KO hippocampus after transplantation with Neural/Precursor Cells (NPCs)
RNA-Seq of WT and Mecp2 KO hippocampus after transplantation with Neural/Precursor Cells (NPCs)
hippocampus dissection from experimental animals Transplantation of neural precursor cells (NPCs, 10 million of cells) or PBS (10 ul) intra-cisterna magna, under anaesthesia RNA was extracted using Purezol (cod.7326890 Biorad). DNasi I amplification Grade (cod.AMPD1, Sigma-Aldrich) was used to remove possible genomic DNA contamination and a second RNA extraction was performed with Purezol. RNA integrity was verified using the RNA 6000 nano Kit on a Bioanalizer (Agilent Technologies). All samples had a RIN>9. RNA was quantified using a Qubit RNA BR Assay Kit. Libraries were prepared according to the following reference guide: /https://emea.support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/illumina_prep/RNA/illumina-stranded-mrna-reference-guide-1000000124518-02.pdf