GSM9378314: Mnk3, CRISPRi, scramble, With stimulation, replicate 2, merged; Mus musculus; RNA-Seq
RNA was harvested by spin column (Qiagen RNeasy kit) for RT-qPCR and RNAseq, in triplicate. Standard polyA selected bulk mRNA seq libraries were generated for Illumina 100bp paired-end sequencing (Illumina platform, University of Cincinnati Genomics, Epigenomics, and Sequencing Core, Cincinnati, OH, USA). RNAseq samples were performed in triplicate.