GSM8721784: HSC_scRNAseq; Mus musculus; RNA-Seq
Ex vivo expanded HSCs were sorted via fluorescence-activated cell sorting (FACS) using an Aria III cell sorter (BD) equipped with a 100 µm nozzle and appropriate filters and settings. Subsequently, nuclei were isolated according to the protocol of the Chromium Nuclei Isolation Kit. Libraries were prepared using the Single Cell 3' Reagent Kit v.3.1 (10x Genomics) and sequenced on an Illumina NovaSeq X platform.