16S metagenome data of Daqu 101 (CRX2176815)
PCR amplification was performed using specific primers designed for the V3-V4 region of the bacterial 16S rRNA gene and the fungal ITS region. Sequencing universal adapters for subsequent library construction and sample-specific Barcodes were added to the amplification primers. After purification, quantification, and pooling, amplicon libraries suitable for the Illumina sequencing platform were constructed for high-throughput sequencing.