RNA-Seq of Gromphadorhina portentosa hemocytes
Total RNA was extracted from hematopoietic organs. The samples were homogenized, and total RNA was isolated using TRIzol reagent. 250 ng of total RNA were treated with VAHTS mRNA Capture Beads 2.0. Subsequently, the mRNA-enriched samples were immediately used to construct NGS libraries using the MGIEasy RNA Library Prep Set, following the manufacturers protocols. The resulting mRNA libraries were sequenced on a DNBSEQ-G50 sequencing machine in paired-end 150 mode.