GSM9238877: CASE_13, type: CASE, diagnosis: adolescent, progression: high; Homo sapiens; miRNA-Seq
The peripheral blood samples were collected in EDTA tubes and centrifuged at 4 °C/10 min/2000 g. Blood plasma was stored at -80 °C. RNA was extracted using the miRNeasy Serum/Plasma Kit (QIAGEN) according to manufacturer's instructions and stored at -80 °C. cDNA libraries were prepared using the QIAseq miRNA UDI Library Kit (QIAGEN). The quantity and quality of the libraries was checked by Qubit 4.0 Fluorometer (Invitrogen) and Tape Station 2200 System (Agilent). Sequencing analysis was performed using the NovaSeq 6000 S2 v1.5 Kit - 100 cycles using the NovaSeq 6000 instrument (both Illumina).