GSM9430547: VE-822 40nM Round-06 (exp300); Homo sapiens; OTHER
genomic DNA extracted using the Gentra Puregene kit according to the manufacturer's instructions (QIAGEN), and sgRNA sequences were PCR-amplified sgRNA libraries were prepared by PCR amplification to add sequencing adapters and indices followed by SPRI beads purification of sequencing-ready amplicons.