YPQ231202hifi-Oct-23
High-molecular-weight genomic DNA is sheared to approximately 15-18 kb, end-repaired, A-tailed, ligated with SMRTbell adapters, size-selected to remove fragments below 10 kb, cleaned and QC-checked, before binding polymerase and running on the Revio system to generate HiFi reads along with methylation and demultiplexing information processed onboard.