SY_2 (CRX2066016; CRA031377)
After Trizol RNA extraction (Invitrogen) and NanoDrop quantification, 3 ug RNA underwent Illumina library prep: poly-A enrichment, cation fragmentation, Superscript II cDNA synthesis, end repair/A-tailing, PE adapter ligation, AMPure XP size selection (400-500 bp), 15-cycle PCR enrichment, Bioanalyzer QC (Agilent), and NovaSeq 6000 sequencing (Illumina)