GSM8463100: THP1, GLUT3oe, EXOSOME, 24hr, repA; Homo sapiens; RNA-Seq
RNA was isolated using Norgen Total RNA kit plus DNase treatment according to the manufacturer's protocol (Norgen # 37500, 25720). RNA quality was verified with the Agilent BioAnalyzer RNA Nano 600 kit (cat# 5067-1512) with the RIN range between (9.7–10 RIN). RNA was quantified using Qubit 2.0 Fluorometer (Thermofisher). RNA-sequencing libraries were made using Lexogen QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina kit (cat# 015.24) with 250 ng of RNA input.We pooled all samples for sequencing on a NextSeq 500 with single-end 75 base pair reads. Read counts averaged 3.9 million reads per sample with an average Q30>92%.