GSM8895452: RM2 macrophages; Mus musculus; RNA-Seq
Total RNA was extracted from polarized and irradiated macrophages using WelPrep™ Total RNA Isolation Reagent (Welgene, Gyeongsan, Korea) according to the manufacturer's instructions. Total RNA was fragmented, mRNA was enriched or depleted, followed by reverse transcription and second strand synthesis. After adapter ligation and PCR amplification, ssCircs were converted into DNA nanoballs (DNBs) for PE100/PE150 sequencing.