GSM9571681: BMDM, NT,48hrs, rep4,R1; Mus musculus; RNA-Seq
RNA was extracted using the Qiagen RNeasy Mini Kit 500 according to the manufacturers' protocol RNA libraries were prepared with KAPA stranded mRNA-Seq kit. High throughput sequencing was performed on Illumina NovaSeq 6000 (paired end, 2x150bp) targeting 100 million reads per sample. Demultiplexing was performed with Illumina Bcl2fastq v2.19.1. Gene expression quantification from the resulting fastqs was performed using Salmon v1.21.1 in mapping-based mode. Reads were selectively aligned to the GENCODE vM25 mouse reference transcriptome with corrections for sequence-specific and GC content biases.