RNA-seq of mouse: adult male hippocampus
RNA-seq was performed on hippocampal tissue from Mus musculus (B6-Tfam-flox/Slc6a3-Cre) mice. Animals were divided into four groups: control (vehicle, G6) and treatment groups receiving nanoparticle formulations (AT1, AT2, AT3; G7-1, G7-2, G7-3). Each group included five biological replicates. Total RNA was extracted and mRNA was enriched using Oligo(dT) magnetic beads. Libraries were constructed and sequenced on an Illumina NovaSeq 6000 platform using paired-end reads. Differential gene expression analysis was performed to evaluate transcriptional changes associated with treatment.