recovered OTUs Malaise traps LandKlif
Malaise traps were active on 4 land-use types: forest, grassland, arable field, settlement. DNA extraction from arthropod samples was conducted by incubating them in a 90:10 solution of animal lysis buffer (buffer ATL, Qiagen DNEasy tissue kit, Qiagen, Hilden, Germany) with 10% proteinase K following the standard Qiagen DNeasy protocol. Amplicon PCRs employed 5 L of extracted genomic DNA, Plant MyTAQ (Bioline, Luckenwalde, Germany), and HTS-adapted mini-barcode primers targeting the mitochondrial Cytochrome-Oxidase subunit I (CO1-5P) region.