GSM8894722: Experiment2_KO1; Mus musculus; RNA-Seq
E0771.lmb tumors were grown after tamoxifen injection as previously described in wild-type and Shb iECKO (Shb flox/flox; Cdh5-CreERt2) mice, alternatively Shb ICKO (Shb flox/flox; Vav1-cre) mice. Mice were sacrificed on day 14-16 after tumor cell injection. The tumors were digested, CD45+-cells isolated by MACS microbeads (Miltenyi, Bergich Gladbach, Germany)  and subsequently CD31+-cells isolated by fluorescence-activated cell sorting (FACS) after staining for CD31. Libraries were prepared from 30ng total RNA using the Illumina Stranded Total RNA library preparation kit with Ribo-Zero Plus treatment (cat# 20040525/20040529, Illumina Inc.). Unique dual indexes (cat# 20040553/20040554, Illumina Inc.) were used. The library preparation was performed according to the manufacturers' protocol (#1000000124514). Paired-end 150bp read length were sequenced.