Transcriptomic analysis of the knockdown effects of LRRC41 and DDX5
Transcriptomic analysis of the knockdown effects of LRRC41 and DDX5
The cells were maintained in Dulbeccoâ€™s Modified Eagle Medium and Nutrient Mixture F-12 (DMEM/F-12; Gibco, USA), supplemented with 10% fetal bovine serum (FBS;ScienCell, USA), and 1% penicillin-streptomycin solution (NCM Biotech, China).  RNA was extracted from transfected SSC cells using Total RNA Extraction Reagent (Vazyme, Nanjing, China) according to the manufacturer's instructions.  The RNA extracted from the cells was initially subjected to quality control. mRNAs with poly(A) tails were enriched using oligo(dT) magnetic beads. These mRNAs were then randomly fragmented using divalent cations in a specified buffer, and library construction was performed following the NEB standard library construction protocol. 