GSM8513514: BV-2, BHB_3; Mus musculus; RNA-Seq
Total RNA was extracted from the BV-2 cell samples of each experimental group according to the instructions of the TRIzol reagent kit. The cDNA libraries were prepared using the SmartScribeTM Reverse Transcriptase Kit (TAKARA, Japan). Ampure XP Beads (Beckman Coulter, USA) were used to select 300-450 bp DNA fragments from the indexed libraries. The libraries were quantified using an Agilent 2100 Bioanalyzer and then combined at a concentration of 2 nM in a 1:1 ratio.