GSM8862519: PEO1, sipNC, E2, 0h, 2; Homo sapiens; RNA-Seq
Total RNA was extracted from cells using QIAshredder columns (QIAGEN) and RNeasy Mini kit (QIAGEN), followed by DNase digestion. Libraries for RNA-seq were prepared using the Quant Seq 3' mRNA-Seq V2 Library Prep Kit FWD (Lexogen, Vienna, Austria) as per manufacturer's instructions starting with an input of 200ng of RNA and 16 cycles of final PCR amplification.  Overall library size was determined using the 4200 Tapestation and the High-sensitivity DNA 5000 assay (Agilent, Santa Clara, CA) and libraries were quantitated using the Qubit 2.0 Fluorometer (Thermo Fisher, Waltham, MA).