RNA-Seq of Saccharina japonica
To elucidate the molecular mechanisms of perchlorate toxicity in Saccharina japonica, male and female gametophytes were subjected to perchlorate stress for 48 hours, with deionised seawater serving as the control. The experimental design included four groups (CK-M, CK-FM, Per-M, and Per-FM) with three independent biological replicates for each. Total RNA was isolated from the gametophyte samples and sequenced on an Illumina HiSeq platform to generate 150 bp paired-end reads, enabling a comprehensive transcriptomic analysis to identify differentially expressed genes (DEGs) and characterize the transcriptional reprogramming associated with perchlorate-induced growth inhibition and oxidative stress.