GSM8942517: FL_F_495_citrate; Mus musculus; RNA-Seq
RNA was isolated using TRIzol (Thermo Fisher Scientific, 15596018) according to the manufacturer's instructions. After RiboGreen quantification and quality control by Agilent BioAnalyzer, 500 ng of total RNA with RIN values of 7.3-10 underwent polyA selection and library preparation using the TruSeq Stranded mRNA LT Kit (Illumina catalog # RS-122-2102) according to instructions provided by the manufacturer with 8 cycles of PCR.