GSM8895465: K562_DNA_Replicate_2_R1; Homo sapiens; OTHER
Electroporated cells, collected post 6 hours were subjected to RNA and DNA isolation using TRIzol reagent (Cat#15596026). Isolated RNA was then DNase treated using TURBO DNA-free™ Kit (Cat# AM1907). cDNA was further prepared using SuperScript™ IV Reverse Transcriptase (Cat# 18090010). STARR-Seq (Other) Pair-ended. Region of interest was amplified from the isolated DNA using the forward primer TTGCCTTTCTCTCCACAGGT and reverse primer GATGAGTTTGGACAAACCACAAC. While region of interest from the reverse transcribed RNA was amplified using the forward primer TAGAGCATGCACCGG and reverse primer GATGAGTTTGGACAAACCACAAC. Amplified fragments were then gel purified before preparing the sequencing library using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®(Cat# 7645S). Libraries thus prepared post-quality control were multiplexed and sequenced.