Whole genome sequencing of Escherichia coli: ESBL-producing isolate from chicken cecal content (Xa La Market, Hanoi, Vietnam)
Genomic DNA was extracted from an overnight culture of Escherichia coli grown on MacConkey agar using a commercial bacterial DNA extraction kit according to the manufacturers instructions. DNA quality and quantity were assessed using a NanoDrop spectrophotometer and Qubit fluorometer. Sequencing libraries were prepared with the Nextera XT DNA Library Preparation Kit following the standard Illumina protocol. Whole genome sequencing was performed on the Illumina MiSeq platform generating paired end reads of 2 x 150 bp.