snRNA-seq of homo sapiens: adult male sorted neurons
Single Nucleus RNA Seq was performed using the 10x Genomics Chromium iX platform and the Chromium GEM-X Single Cell 3' v4 Gene Expression Assay (PN-1000691) with a targeted nuclei recovery of 20,000 nuclei. Libraries were prepared as per the 10x Genomics demonstrated protocol (https://cdn.10xgenomics.com/image/upload/v1725314293/support-documents/CG000731_ChromiumGEM-X_SingleCell3v4_UserGuide_RevB.pdf). All libraries were quantified via Agilent 4200 Tapestation High Sensitivity D5000 ScreenTape Assay (Agilent Cat# 5067-5592, 5067-5593) and KAPA library quantification kit (Roche Cat# 0796014001.) Gene expression libraries were sequenced at a targeted depth of 25,000 reads per cell. Libraries were sequenced on the Illumina platform with run parameters 28/10/10/90 (R1xi7xi5xR2).