E8.5-like (CRX1203673)
Single-cell suspensions(100000 cells per ml) were loaded into a 10X Genomics Chromium Chip following the manufacturer's instructions48(10X Genomics,Chromium Next GEM Single Cell 3'GEM,Library and Gel Bead Kit v.3.1).10X Genomics scRNA-Seq were done by Annoroad Gene Tech.(Beijing) Co.Ltd