GSM9314959: Human cardiac microvascular endothelial cell EVs, HCMVEC-EVs, rep 1; Homo sapiens; miRNA-Seq
miRNA was isolated from lysed EV pellets using the miRNeasy Micro Kit (Qiagen, 217084) according to the manufaturer's protocol. Samples were treated with DNase. ﻿CM-, CF-, HCMVEC-EVs: ﻿SEQuoia Complete Stranded RNA Library Prep Kit and Dual Indexed Primers (Bio-Rad 17005726 and 12011928) Mac-EVs: Library preparation was performed using the QIAseq miRNA Library Kit (Qiagen, catalog no. 331502).