PacBio IsoSeq of male crucian carp: PacBio Sequel II polished ccs reads prepared from total RNA from multiple tissues (brain, liver, red muscle, white muscle, gills, gonad, spleen, kidney, heart)
Extraction of total RNA with Trizol followed by Pacific Biosciences protocol for Iso-Seq Express Template Preparation and ccs v6.0.0 from SMRT tools v10.1 for generation of ccs reads (polished with default parameters).