GSM8513116: WT, scRNA-seq; Mus musculus; RNA-Seq
Freshly collected tumor from WT or MALT1-PD mice bearing glioblastoma (GL261) were enzymatically and mechanically dissociated into a single cell suspension for 15 minutes at 37°C in3.2 mg/mL collagenase type IV, 2 mg/mL soybean trypsin inhibitor, and 1.0 mg/mL deoxyribonuclease I (Worthington Biochemical). Live cells were further isolated from the single-cell suspension using the Dead Cell Removal Kit (Miltenyi Biotec). Three tumors were cell-hashed together into a single suspension using TotalSeq C Antibodies (Biolegend). Library Preparation (Chromium Next GEM Single Cell 3' Reagent kit v3.1) was conducted as per the manufacture's protocol. The quality of the cDNA libraries was verified using a High Sensitivity D5000 ScreenTape and 4200 Tapestation system (Agilent Technologies).