scRNA-Seq of Homo sapiens: NECC  TIL product
Library strategy: Single-cell RNA-seq libraries were prepared from live cells using the 10x Genomics Chromium Next GEM Single Cell 5' Reagent Kit v3.1 following the manufacturer's protocol. Briefly, cells were loaded onto a Chromium Chip for GEM generation and barcoding. Barcoded cDNA was amplified, fragmented, and ligated with adapters to construct the 5' gene expression library.