GSM8862397: IgG_control_R1; Mus musculus; ChIP-Seq
Cortical tissue was homogenized with a douncer in homogenization buffer supplemented with 1 mM DTT, 0.15 mM spermine, 0.5 mM spermidine, RNasin ® Plus Ribonuclease Inhibitor (Promega N2611), and EDTA-free protease inhibitor (Roche). Nuclei were isolated using an iodixanol density gradient and ultradentrifugation. TruSeq ChIP Library Preparation Kit