GSM8829021: E12.5 Mouse Embryo, D1; Mus musculus; OTHER
Fresh frozen tissue were OCT embedded, and 10 µm frozen sections were cut with cryostat. Hematoxylin and eosin (H&E) staining was performed using a kit (ab245880/Abcam, Cambridge, UK) according to the manufacturer's instructions. H&E stained slides were cover slipped using glycerol, scanned using a Hamamatsu NanoZoomer S20 slide scanner system (C16300-01/Hamamatsu, Shizuoka, Japan) at 400x resolution. H&E scanned slides were de-cover slipped after a brief incubation in room temperature nuclease-free water, then given for Visium HD. Spatial transcriptome seqeuncing libraries were prepared with 10X Genomics Visium HD spatial Gene Expression Slides & Reagent kit according to manufacture's construction.