GSM8968590: P7F1_RNAseq_pDOX_Rep2; Homo sapiens; RNA-Seq
For RNAseq, total RNA was extracted from iPS control and iPS-MPPAX3/7-FOXO1 cells (in three technical replicates) using RNA Zymo-Spin columns (Zymo R1015). Before library preparation, rRNA was depleted with NEBNext rRNA Depletion Kit (NEB E6310). RNA-seq libraries were constructed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB E7760) and sequenced (2x50bp paired end) using an Illumina NextSeq 2000 machine.