Rla_RNAseq
Total RNA was extracted by grinding tissue in TRIzol reagent on dry ice and processed following the protocol provided by the manufacturer. The integrity of the RNA was determined with the Agilent 2100 Bioanalyzer (Agilent Technologies) and agarose gel electrophoresis. The purity and concentration of the RNA were determined with the Nanodrop (Thermo Fisher Scientific) and Qubit (Thermo Fisher Scientific). Only high-quality RNA sample (OD260/280=1.8~2.2, OD260/2302.0, RIN8, >1 g) was used to construct sequencing library.