PPLmutRNA2
Cells were harvested in lysis buffer (20 mM Tris-Cl [pH 8], 150 mM KCl, 15 mM MgCl2, 1% Triton X100, 1 mM DTT, 0.1 mg/mL cycloheximide, supplemented with 1X cOmplete Mini Protease Inhibitor Cocktail).