GSM9572879: CONTROL2; Mus musculus; RNA-Seq
RNA was extracted with Total RNA Purification Plus Kit (Norgen Biotek Corp.,48300) following manufacturer instructions Gene expression profiling was carried out using a 3'-Tag-RNA-Seq protocol. Barcoded sequencing libraries were prepared using the QuantSeq FWD kit (Lexogen) for multiplexed sequencing according to the recommendations of the manufacturer using both the UDI-adapter and UMI Second-Strand Synthesis modules (Lexogen).