GSM9433332: naive CD4+ T cells, Nur77-GFP-HI, rep_1; Mus musculus; RNA-Seq
Mouse cells were first pre-enriched by negative selection, using the EasySep™ Mouse naive CD4+ T Cell Isolation Kit. Cells were sorted as singlet, viability dye-, CD4+, CD8-, CD44lo, CD62L+, CD25-, CXCR3- and either GFP low or high expressors. Cells from Mx1-GFP mice were sorted on the Mx1-GFP+, with a gate set using WT mice, which was the top 1% of cells. The Mx1-GFP- population was sorted using a gate set on the bottom 10-15% of naive CD4+ T cells. For Nur77 and KLF2, the top and bottom 10-15% of GFP was sorted. Samples were sorted into buffer RTL. Then, RNA was isolated and prepared for sequencing using the Takara Pico v3 kit