GSM8459917: 250nM estradiol, rep2 [RNA]; Homo sapiens; RNA-Seq
For RNA isolation, total RNA was isolated using TRIzol reagent. A total of 2 μg of RNA/sample was used as input material for the miRNA library. The miRNA sequencing libraries were generated using a TruSeq Small RNA Sample Preparation Kit, and index codes were added to attribute sequences to each sample.