GSM9140077: CO_MECP2-WT,R133C,R168X,R270X_Rep1,2,3_L1; Homo sapiens; RNA-Seq
Organoids were gently dounced in sucrose-based buffer with detergents and RNase inhibitors, filtered, pelleted, washed, counted, and immediately fixed using the Evercode™ Nuclei Fixation v2 kit. Eight single-nucleus libraries were prepared with the EVERCODE™ WT v2 kit (Parse Biosciences, #ECW02135) according to the manufacturer's split-pool protocol.