GSM9430122: hTERT-RPE1, RB KO, G1; Homo sapiens; RNA-Seq
RBWT and RBKO RPE1 cells arrested in G1 phase by 6 days of contact inhibition were collected for single-cell RNA sequencing (scRNAseq). Cells were washed twice with RPMI containing 10% heat-inactivated FCS, filtered through a 50 μm filter (Sysmex, Cat#04-004-2327), followed by cell concentration and viability assessment using trypan blue with a Bright-Line hemocytometer (Cat#1492), with both samples reaching 95% cell viability. Gene expression libraries were constructed using the 10x Genomics GEM-X Universal 3' Gene Expression v4 reagent kits (1000691, 1000690, and 1000215) according to the manufacturer's guidelines. Quality control was assessed for both cDNA and gene-expression libraries using Qubit dsDNA High Sensitivity Kit (Invitrogen, Cat#Q32854) and the Agilent High Sensitivity Bioanalyzer DNA Kit (Cat#5067-4626).