tRNA-seq MRT12_G
Gel extracted (G) tRNA, followed mim-tRNA-seq library preparation workflow using mim-seq indexed RT adaptors, reverse transcribed by MRT (MRT12_G). Sequencing was performed in paired-end mode on an Illumina platform. Due to short tRNA insert sizes, only Read 1 was used for downstream analysis. Secondary demultiplexing (RT-adaptor indices) of the individual RT reactions was preformed by user according to mim-seq workflow.