DNA short read sequencing of Acinetobacter from Onthophagus taurus
Samples were lysed with MAGMAX Microbiome bead beating tubes before DNA extraction using Qiagen DNeasy 96 PowerSoil Pro QIAcub HT Kit #47021. Illumina reads were prepared using an Illumina DNA prep tagmentation kit and unique dual indexes. Sequencing was performed on the Illumina NextSeq2000 platform using a 300-cycle flow cell kit to produce 2x150bp paired reads. 1-2% PhiX control was spiked into the run to support optimal base calling. Read demultiplexing, read trimming, and run analytics were performed using DRAGEN v4.2.7 on the NextSeq2000.