ITS Crohn_B2 DO82
The hypervariable region ITS1 of the ITS gene was amplified using universal primers (forward ITS5 5-GGAAGTAAAAGTCGTAACAAGG-3 and reverse ITS2 5-CTRYGTTCTTCATCGDT-3). The Metagenomic Sequencing Library Preparation Illumina protocol was used for library preparation. Sequencing was performed at FISABIO Institute (Valencia, Spain) using 2  300 bp paired-end sequencing with an Illumina NextSeq instrument.