GSM9683861: Bulk_RNA-seq_siControl_3; Homo sapiens; RNA-Seq
Total RNA was extracted 48 hours post-transfection using the RNeasy Mini Kit (Qiagen) following the manufacturer's protocol. RNA quality and integrity were assessed using a Bioanalyzer. Samples with RNA Integrity Number (RIN) ≥ 8.0 were used for library preparation. RNA-seq libraries were prepared using the TruSeq Stranded mRNA Library Prep Kit (Illumina) and sequenced on an Illumina NovaSeq 6000 platform to generate 100 bp paired-end reads.