GSM9571709: Pcbp1/2-dKO; Mus musculus; RNA-Seq
Sorted cells were resuspended in PBS containing 0.4% FBS, and the concentration was adjusted to target approximately 10000 cells per sample. Cells were loaded onto a Chromium Next GEM Chip G together with reverse transcription master mix and Single Cell 3' v3.1 Gel Beads to generate gel beads-in-emulsion (GEMs). Within each GEM, reverse transcription produced barcoded cDNA, which was subsequently amplified, fragmented, and subjected to adaptor ligation and sample index attachment. Final libraries were sequenced on an Illumina NovaSeq 6000 platform.