GSM8862811: nuclei from cells treated with Ad-Dam-3HA-eGFP, MNase rep 2; Homo sapiens; MNase-Seq
Nuclei from MCF7 cells (treated with Ad-Dam-3HA-eGFP for 72h) were treated with MNase, digested DNA was purified using the Purelink genomic DNA kit (K182002) from Invitrogen and analysed on agarose gel. For accurate nucleosome mapping we chose digests with the only major band at ~150 bp. Paired-end libraries were prepared using the New England Biolabs kits and protocols. Ocampo et al., 2016, Nucleic Acids Res. 2016 Jun 2;44(10):4625-35.