GSM8942160: LU_RAS_D2_H3K27AC_IP; Homo sapiens; ChIP-Seq
2 ug of total RNA was used for the construction of sequencing libraries.For ChIP-seq, cells were fixed with 1% formaldehyde for 10 minutes.Then terminate crosslinking with glycine. Add Micrococcal Nuclease to each IP preparation, mix by turning the tube several times and incubate at 37°C for 20 minutes with frequent stirring to digest the DNA to approximately 150-900 bp in length. The ChIP-Seq library preparation was proceeded as follows: (1) The DNA fragments were end-repaired and A-tailed. (2) The DNA fragments with A tail were ligated with sequencing adaptors. (3) The final DNA library was obtained after size selection and PCR amplification. Libraries were analyzed for size distribution by Agilent 5400 system（Agilent, USA）and quantified by QPCR (1.5 nM). The qualified libraries were pooled and sequenced on Illumina platforms with PE150, according to the effective library concentration and data amount required