Identification of Differentially Expressed miRNAs Involved in Vascular Aging and Association with the Endocrine Hormone Regulation Pathways
Identification of Differentially Expressed miRNAs Involved in Vascular Aging and Association with the Endocrine Hormone Regulation Pathways
We used the data from the Namgaram project consisting of a total of 1,010 subjects at the age of 50 or above, recruited from the southern site (Jinju) in Korea, all the individuals were measured for age-related qualitative and quantitative traits, including age, grip strength, muscle strength score, and vascular endothelial functions. We randomly selected 3 samples from the top 10% and bottom 10% of RHI in each age group of 50s and 80s, and performed small RNA sequencing on a total of 12 samples. Exosomes were isolated from serum using the Exo2Dâ„¢-EV isolation kit for RNA analysis (for plasma/serurn). RNA was then isolated using the miRNeasy Serum/Plasma Advenced Kit (217204). Individual libraries were generated SMARTer smRNA-Seq for Illumina Kit (Illumina, San Diego, CA, USA)