GSM8862536: SCA27_MeWo Young replicate 1; Homo sapiens; RNA-Seq
RNA from cells were extracted using Qiagen RNA extraction kit (Qiagen, Cat no. 74104) as per manual instructions. 1ug RNA used per condition. PolyA RNASeq libraries were prepared using the SureSelect Automated Strand-Specific RNA Library Prep for Illumina (Agilent, G9691B). An input of 200ng of total RNA was used with 14 cycles of amplification.