WGS of Alpinia ligulata
Sequencing libraries were constructed using the xGen DNA Library Prep EZ UNI kit (Integrated DNA Technologies, IA, USA). Enzymatic fragmentation of gDNA yielded inserts averaging 350 bp in size. Libraries were quantified and validated for size distribution using the Agilent 4150 TapeStation. Paired-end sequencing (150 bp read length) was performed on an Illumina NextSeq 500 platform (Illumina, CA, USA) at Genetika Science Lab (Tangerang, Indonesia), generating approximately 10 Gb of raw sequencing data to ensure sufficient coverage for chloroplast genome assembly.