GSM8794695: Blood, FF030BASO_B3; Mus musculus; RNA-Seq
0.5–1 mL mouse peripheral blood was collected into EDTA coated tubes. Red blood cells were lysed using RBC lysis buffer (Sigma-Aldrich). All live, CD45+Lin-CD49b+FcεRIα/IgE+ basophils from WT mice were sort-purified on a FACSAria cell sorter (BD Biosciences). Isolated basophils (2 × 104 – 5 × 104 cells /mL) were then conducted for the transcriptome sequencing using the Smart-seq2 protocol. A cDNA library constructed by technology from the pooled RNA was sequenced at the Illumina NovaseqTM 6000 sequence platform (LC Sciences, USA). The Illumina paired-end RNA-seq approach was employed, generating 2 × 150 bp paired-end reads, with a total yield of approximately 1 million reads per sample.