GSM8829500: SA19881; Canis lupus familiaris; RNA-Seq
Total RNA was extracted from the epithelium tissues using the Invitrogen TRIzol® Plus RNA Purification Kit. Four samples were extracted in two separate batches. cDNA libraries were constructed using the KAPA mRNA HyperPrep Kit with dual indices (Kapa Biosystems, Ltd). Individual libraries were then pooled in equimolar ratios and sequenced on two lanes of an Illumina Hiseq4000 (150bp paired-end). Sequencing was performed at Fulgent genetic (https://www.fulgentgenetics.com/).