GSM9756834: MOD1; Rattus norvegicus; RNA-Seq
Total RNA was isolated using the Trizol Reagent (Invitrogen Life Technologies). After isolation, the concentration, quality and integrity were determined using a NanoDrop spectrophotometer (Thermo Scientific). mRNA Purification,Fragmentation,First-Strand cDNA Synthesis,Second-Strand cDNA Synthesis,End Repair,Adenylation,Adapter Ligation,Size Selection,Enrichment by PCR ,Final Purification and Quantification