The genome of Methicillin-resistant Staphylococcus aureus (Sam21) was sequenced using Oxford Nanopore Technologies sequencing, producing a high-quality ~2.85 Mb assembly. Subsequent bioinformatics analyses identified resistance genes, sequence type (ST6), and mobile genetic elements.
Genomic DNA from Methicillin-resistant Staphylococcus aureus (Sam21) was extracted, quality-checked, and prepared for sequencing using Oxford Nanopore Technologies sequencing through end-repair, adapter ligation, and purification. The library was then sequenced, followed by basecalling and quality filtering for downstream analysis.