GSM8896087: NM1 WT blood 01; Mus musculus; RNA-Seq
Spleen was isolated from euthanized mice and cleaned from surrounding fat and connective tissue. Approximately 5mm3 of tissue from the tip of the spleen was merged in RNAzol and homogenized in bead mill homogenizer followed by standard RNA isolation protocol. Blood samples were collected by submandibular vein bleeding according to a previously published protocol (Golde et al, 2005). Collected blood was immediately mixed with the double amount of RNAzol and samples were thoroughly mixed and stored on dry ice before RNA isolation by standard protocol. The final RNA concentration was measured by the Qubit RNA BR Assay Kit. Three replicates were prepared for each genotype. 500ng of total RNA was used for the RNA-Seq library preparation by using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB) and sequenced with the NextSeq 500/550 sequencing platform (performed at the NYUAD Sequencing Center).