GSM8830521: neo teno, adultsn, rep2; Mus musculus; RNA-Seq
Trizol/chloroform extraction was used to isolate RNA.  The RNA integrity number (RIN) and concentration was assessed with the Bioanalyzer pico chip at the Columbia University Molecular Pathology Shared Resource. Standard RNA-seq on total RNA with polyA tail mRNA selection was completed by Azenta Life Sciences (20M reads per sample) with Illumina 2x150bp technology, with all samples having RIN scores >6. NEBNext Ultra II RNA Library Prep Kit