WGS of Burkholderia pseudomallei
First, the Covaris G-tube was used to randomly fragment the DNA sample into 15-kilobase pairs (kb) fragments. Subsequently, the PacBio library preparation kit was used to remove single-stranded overhangs and repair DNA damage in the fragmented DNA. Dumbbell adapters were ligated to both the 3' and 5' ends of the DNA fragments, and excess fragments were degraded using exonuclease. Nucleic acid fragments were then purified with AMpure PBmagnetic beads (PACBIO, USA).