GSM9894441: NES cells, MYCN(T58A) uninduced (dox), rep 1; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNeasy Kit (Qiagen) per manufacturer's instructions (pelleted cells for in vitro samples; snap-frozen tumour for NESt). Samples passed QC: total RNA >= 200 ng, concentration >= 20 ng/µL, OD260/280 and OD260/230 >= 1.8, RIN >= 7. Stranded mRNA libraries were prepared by BGI (DNBSEQ Eukaryotic Strand-specific mRNA library).