GSM9573408: cortical human organoids, P2, medium heteroplasmy; Homo sapiens; RNA-Seq
Single-cell suspensions were generated from human iPSC-derived cortical organoids. Organoids were dissociated into single cells using enzymatic digestion followed by mechanical trituration. Cells were filtered through a 40 µm strainer, counted, and viability assessed prior to library preparation. Single-cell RNA-seq libraries were prepared using the 10x Genomics Chromium Single Cell 3' v3.1 platform according to the manufacturer's protocol. Single cells were encapsulated in droplets with barcoded gel beads using the Chromium Controller, followed by reverse transcription, cDNA amplification, and library preparation. Libraries were sequenced on an Illumina NovaSeq 6000 platform.