GSM8461015: Kidney, Dmrt2 KO, rep1; Mus musculus; RNA-Seq
Samples were preserved in RNAlater, incubated overnight at 4°C and stored at -80°C until extraction Whole embryonic kidney RNA was extracted using Qiagen RNeasy Mini Kit according to manufacturer's protocols. RNA was eluted in 30uL nuclease free water. Samples were shipped to the Genome Technology Access Center at McDonnell Genomic Institute at Washington University in St. Louis for sequencing. Up to 5ug of RNA were used for library prepration; library preparation was performed using the manufacturer's protocol for polyA selection.