GSM8830294: het-KO-rep2; Mus musculus; RNA-Seq
Total RNA was extracted from sorted CD4+YFP+ Treg cells using the RNeasy Plus Mini Kit (Qiagen, #74134), following the manufacturer's protocol. RNA-Seq libraries were constructed and sequenced by Haplox (Nanchang, China), using an Illumina platform with paired-end reads of 150 bp.