RNA seq of tomato hairy root cultures induced with the nuclear-lokalized effector RIR190 of R. irregularis
RNA seq of tomato hairy root cultures induced with the nuclear-lokalized effector RIR190 of R. irregularis
tomato hairy root cultures were generated via the cotyledon hairy root transformation protocol. Hairy roots were subcultured on MS. After transgene root selection, roots were grown in liquid MS, and after 3 weeks treated with 100 um estradiol and harvested 24 hours after treatment tomato hairy root cultures in falcons grown on a shaker with 200 rpm tomato hairy root cultures treated with 100 um estradiol and harvested 24 hours after treatment Total RNA was extracted via the ReliaPrep RNA Miniprep Systems  DNBSEQ stranded mRNA library processed through BGI