Bos taurus Hanwoo PBMC Treponema spp. antigen stimulated sample from Animal6
Peripheral blood mononuclear cells (PBMCs) were isolated from clinically healthy Hanwoo cattle. This library was prepared from Animal6 under the Treponema spp. antigen-stimulated condition. For each animal, PBMCs were divided into paired unstimulated and stimulated aliquots. Stimulated samples were incubated for 12 h with a combination of whole cell sonicates from three bovine digital dermatitis-associated Treponema species. Libraries were prepared using the TruSeq Stranded Total RNA Library Prep Gold Kit with rRNA depletion and sequenced as 101 bp paired-end reads on an Illumina NovaSeq 6000 platform.