Nanopore sequencing of Homo sapiens: whole blood DNA from normoglycemic adult (Follow-up)
Whole-blood genomic DNA was extracted from adult participants classified as normoglycemic, prediabetic, or type 2 diabetes mellitus (T2DM) at both baseline and follow-up. Sequencing libraries were prepared using the Oxford Nanopore Technologies Ligation Sequencing Kit following the manufacturers protocol, without PCR amplification, to preserve native 5-methylcytosine (5mC) signals. The barcoded libraries were sequenced on the PromethION platform to generate single-end long reads for genome-wide methylation analysis