RNA-seq of camta123 and mkk45 mutants before and after touching
RNA-seq of camta123 and mkk45 mutants before and after touching
In vitro grown Plants were either untreated, or brushed gently for about 30 seconds per plate and then collected 22 minutes later. Whole seedlings were collected with tweezers and snap frozen in liquid nitrogen. Samples were then ground to a fine powder while frozen before RNA extraction Plants were grown for 12 days on 1/2 Murashige Skoog media in a growth chamber with 16h/8h day/night cycle, 100 umol photons -s -m2 at 22 degrees Celcius. Plants were gently brushed with a soft art brush for about 30 seconds per plate Samples were ground to a fine powder while frozen before RNA extraction. RNA was prepared using the Sigma Spectrum Plant RNA kit The library was prepared according to Illumina instructions for mRNA RNA-seq