GSM8895099: XW_m_2; Arabidopsis thaliana; RNA-Seq
Seven-day-old seedlings grown on half-strength MS plates were harvested, flash-frozen in liquid nitrogen, and ground to a fine powder. Total RNA was isolated using the Plant RNA Kit (Omega, R6827). For RT-qPCR, 0.5 μg of total RNA was reverse-transcribed using the M5 Super plus qPCR RT Kit (Mei5bio, MF166-plus-01), and 1 μL of the resulting cDNA was used for qPCR on a Biorad CFX96 Touch system. RNA sequencing was carried out using the Illumina NovaSeq 6000 with Hiseq-PE150 strategy, conducted by Novogene Corporation (Beijing, China).