GSM9786042: FACS-sorted VT+ xrTestes (VT+ xrTestes), small RNA-seq, R3; Homo sapiens; ncRNA-Seq
Total RNA-seq and small RNA-seq libraries were generated for induced Pluripotent Stem Cells (iPSCs), primordial germ cell-like cells (PGCLCs), FACS-sorted VT+ xrTestes (VT+ xrTestes) and xrTestes (total), with three biological replicates per sample. RNA was isolated using the PureLink miRNA Isolation Kit (Thermo Fisher Scientific) according to the manufacturer's instructions. The long RNA fraction (>200 nt) was retained and further purified with TRIzol. Small RNAs were extracted using the same kit in combination with TRIzol, following the manufacturer's protocol. RNA-seq libraries were prepared using the NEBNext Ultra II directional RNA library Prep kit with rRNA depletion following the manufacturer's instructions. Small RNAs for small RNA-seq were prepared for sequencing as described in Konstantinidou et al. (2024).