GSM8656022: HCT116 p53-/- WT, rep1, control [RNAseq]; Homo sapiens; RNA-Seq
RNA-seq libraries were prepared as previously(Su et al., 2022). Total RNAs (1 μg) were poly-A selected by NEBNext poly(A) mRNA Magnetic Isolation Module (NEB #E7490) and followed by NEBNext UltraII Directional RNA Kit (NEB #7765). The resulting libraries were pooled and sequenced as paired-end reads on Illumina HiSeq2000 (Novogene) or NextSeq (UVA GATC core facility, RRID: SCR_018883) with >20 million reads per sample. polyA RNA-seq