genome-seq of Anomala mongolica: adult without gut
A total of twenty adult females of A. mongolica were processed, with their guts removed to minimize potential microbial contamination. Subsequently, these specimens were promptly flash - frozen in liquid nitrogen and stored at -80C, in preparation for genome sequencing. Genomic DNA for Illumina and PacBio HiFi sequencing were prepared by the SDS method. Single A. mongolica female was used for Illumina sequencing. DNA extraction and DNA library for Illumina sequencing was accomplished by Novogene Company (Beijing, China) and loaded on the XPlus-Illumina platform. After using Fastp (version 1.1.0) to filter out adapter sequences and low-quality reads.8, 9 Finally, 35.46 Gb (74.28coverage) raw reads were generated (Table 1). Single A. mongolica female was used for PacBio HiFi sequencing and Hi-C sequencing. DNA extraction and library construction were accomplished by Novogene Company (Beijing, China) and loaded on the PacBio Revio platform and XPlus-Illumina platform, respectively. After using Fastp (version 1.1.0) (https://github.com/OpenGene/fastp) to filter out adapter sequences and low-quality reads.8, 9 Finally, 81.10 Gb (169.88coverage) PacBio HiFi raw reads and 73.00 Gb (152.96 coverage) Hi-C raw reads were generated, respectively