In this study, we employed Oxford Nanopore long-read whole-genome sequencing to characterize four multidrug-resistant clinical K. pneumoniae isolates, enabling comprehensive resolution of their resistome composition, plasmid architecture, mobile genetic elements (MGEs), and phylogenomic relationships. Multilocus sequence typing identified isolates belonging to ST147, ST11, and the relatively underreported ST7518.
All isolates exhibited extensive multidrug resistance, with resistome profiling revealing co-occurrence of key carbapenemase genes, including bla<sub>NDM-1</sub>, bla<sub>NDM-5</sub>, bla<sub>KPC-2</sub>, and bla<sub>OXA-48-like</sub>. Notably, ST11 isolates co-harbored bla<sub>NDM</sub> and bla<sub>KPC</sub>, while the ST7518 isolate carried bla<sub>NDM-5</sub> in combination with bla<sub>OXA-48-like</sub>, highlighting the emergence of multi-carbapenemase hybrid genotypes within single genetic backgrounds.