Paired VDJ sequencing from PA virulence factor specific B cells from cystic fibrosis patients
cDNA was generated using the Takara Smart-seq v4 kit for single B cells. A multiplex PCR was performed on cDNA from each B cell using pooled constant region primers for IgM, IgG, IgA, IgD, IgK, and IgL constant regions and well-specific barcoded forward primers for the universal template switch region. Half plates were then pooled and library prepped using enzymatic fragmentation and ligation with an anchored multiplex PCR approach to retain well-specific barcodes.