GSM8723394: DRG CPIP+Veh seq2; Rattus norvegicus; RNA-Seq
Ipsilateral L4-L6 DRG were surgically removed after modeling 7 days and enzymatically preserved in RNAlater (Thermal Fisher Scientific, USA). Total RNA was extracted from DRG with Trizol reagent (Thermal Fisher Scientific, USA). The concentrations and the purity of DRG samples were evaluated using NanoDrop Spectrophotometer. RNA integrity number (RIN) was determined by Agilent TapeStation System (Agilent Technologies, USA) and only RIN ≥ 8.0 and A260/230 ≥ 1.5 were used for RNA-Seq. Samples were sequenced by BGISEQ-500 from BGI Group (Shenzhen,China). Distances to expressed genes were calculated using the Euclidean method. RNA libraries were prepared for sequencing using standard Illumina protocols