RNA-Seq of zaozhuang heigai:liver tissue
Total RNA was extracted from liver tissue using the silica membrane method. RNA integrity and purity were assessed via agarose gel electrophoresis and Nanodrop; RNA concentration was accurately quantified with Qubit. mRNA libraries were constructed using the MGIEasy RNA Library Prep Kit (BGI, Shenzhen, China): mRNA was enriched by oligo(dT) magnetic beads, fragmented, then converted to cDNA. The library was prepared through end repair, A-tailing, adapter ligation, PCR amplification, and product circularization. Paired-end sequencing was performed on the BGI DNBSEQ-T15 high-throughput platform.