GSM8461014: CG11247 (Saft)-AID MNase-seq, 48h auxin, rep2; Drosophila melanogaster; MNase-Seq
MNase-seq in the Saft-AID cell line was performed as described previously (Hendy et al., 2022) in two biological replicates, starting with 50 million cells per sample and condition and using 1% (instead of 0.75%) NP-40 for nuclei permeabilization. The MNase reaction with 0.1 units of micrococcal nuclease (Merck) per sample was carried out for 25 minutes at 37°C, carefully vortexing samples every 5 min. Digested DNA was purified and size-selected as described previously (Hendy et al., 2022). DNA libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) following the manufacturer's protocol.