GSM9893615: DM_L_0_F_R4; Triticum aestivum; RNA-Seq
For RNAseq, 64 plants were grown, with four biological replicates per seed treatment, temperature, and time of sampling combinations. Prior to and at 8, 16, and 24 hours after heat exposure began, the unemerged heads were collected. The unemerged wheat heads were removed from the leaf sheath and dissected into rachis and floret tissues, flash frozen,  and stored at -70°C.  Tissue was ground with 3 mm sterilized glass beads in a bead beater, RNA extraction was performed with the RNeasy Plant Mini Kit (Qiagen, Cat #74903) using Beta-mercaptoethanol, and samples were treated with DNase I (Turbo DNA-free Kit, Invitrogen AM1907). Quality of the samples was confirmed via Bioanalyzer using the Agilent RNA 6000 Nano Kit (Agilent, 5067-1511). Five total samples were removed for poor or low RNA quality, leaving a minimum of three biological replications per treatment combination. RNA-seq libraries were prepared using poly(A)-selected mRNA, and RNA-sequencing was performed by Genewiz.