metagenome of sediments
Bacterial 16S ribosomal RNA gene targeted sequencing was performed using the Quick-16S NGS Library Prep Kit (Zymo Research, Irvine, CA). In most cases, the bacterial 16S primers amplified the V3-V4 region of the 16S rRNA gene. These primers have been custom-designed by Zymo Research to provide the best coverage of the 16S gene while maintaining high sensitivity. Fungal ITS gene targeted sequencing was performed using the Quick-16S NGS Library Prep Kit with custom ITS2 primers substituted for 16S primers.