GSM8723573: M1+0.001pct1-4; Homo sapiens; RNA-Seq
The integrity of total RNA was detected by agarose gel electrophoresis, and NanoDrop ND-1000 was used for quantitative and further quality inspection. 1 ~ 2 μg total RNA was selected from each sample for sequencing library construction. The total RNA was first enriched with NEB Next ® Poly (A) mRNA Magnetic Isolation Module (or RiboZero Magnetic Gold Kit for rRNA removal). The treated product RNA was used to construct the library with KAPA Stranded RNA-Seq Library Prep Kit ( Roche ).