Microbiome of bovine intestine: C8_Jejun_Mycopar
A total of 29 calves were assigned to five experimental groups consisting of vaccinated, challenged, and control animals. Duodenum, jejunum, and ileum tissue samples were collected at necropsy 14 months after birth. DNA was extracted from the intestinal samples, and the V4 region of the bacterial 16S rRNA gene was amplified and sequenced on an Illumina MiSeq platform using 2  250 bp paired-end sequencing (MiSeq v2 500-cycle kit). The resulting sequences were generated to characterize the intestinal microbiota and evaluate the effects of vaccination and Mycobacterium avium subsp. paratuberculosis challenge on microbial community composition.