epiGBS data of leaf
For epigenetic genotyping of fifteen Phytolacca americana populations, we employed a reduced - representation bisulfite sequencing (epiGBS) approach, using EcoRI and NlaIII for digestion, EZ - 96 DNA Methylation - Gold MagPrep (Zymo) for bisulfite conversion, constructing libraries with Illumina TruSeq DNA Library Preparation Kit (Illumina) following the epiGBS protocol, and sequencing on the Illumina HiSeq 2500 platform.