GSM8828534: deltaH1-6; Callithrix jacchus; RNA-Seq
For iRICC experiment, total RNA was extracted with TRIzol following the manufacturer's protocol (Invitrogen).  Poly A+ RNA was selected with oligo-dT magnetic beads following the manufacturer's protocol (New England Biolabs). Ten percent of each sample was saved as input and stored in TRIzol.  Crosslinked HSUR1-mRNA complexes were captured with HSUR1 antisense oligonucleotide and magnetic streptavidin beads (Dynabeads).  Bound RNA was released from the beads with TRIzol.  HSUR1 and control pull down samples were purified and used directly to library preparation. Libraries for iRICC experiments were prepared with the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara). For RNA-seq experiments, libraries were prepared with the  NEBNext Ultra II Directional RNA Library Prep with poly(A) mRNA Isolation.