MEPP-OV (CRX1571352)
After cells were lysed in the microwells, mRNA molecules hybridized with the barcoded capture oligos on the beads. These beads were then gathered into a single tube for reverse transcription and ExoI digestion. During cDNA synthesis, each cDNA molecule was tagged at the 5' end (corresponding to the 3' end of the mRNA transcript) with a unique molecular identifier (UMI) and a cell barcode, which was used as the cell origin. The whole transcriptome library was constructed with the NovelCyto single-cell whole-transcriptome amplification (WTA) workflow, including random priming and extension, amplification PCR, and WTA library index PCR. The libraries were quantified with a High Sensitivity DNA chip (Agilent) on a Bioanalyzer 4200 and a Qubit High Sensitivity DNA assay (Thermo Fisher Scientific). All libraries were sequenced by DNBSEQ-T7 Sequencer (MGI, Shenzhen, China) on a 150 bp paired-end run.