GSM6654348: gPlexD_ATAC; Homo sapiens; ATAC-seq
PBMCs were isolated from patients and healthy volunteers by Ficoll density gradient centrifugation. Cells were cropreserved in 10% DMSO + 90% FCS and thawed for use on the day of the experiment. Cells were enriched for CD34+ via MACS twice, before being FACS sorted for CD34+CD45+ live singlets, and then nuclei were isolated for 10X multiome 10X multiome protocol scRNA-seq + scATAC-seq