GSM8827747: L1236_H2ABR7me2a_rep1; Homo sapiens; OTHER
[CUT&RUN] The targeted DNA fragments were isolated and purified following the CUT&RUN protocol developed in Henikoff lab (Skene et al. 2018). Drosophila spike-in DNA was added to each sample for normaliztion. [CUT&RUN] The sequencing libraries were prepared using NEBNext Ultra II DNA library prep kit for Illumina following the kit manual with modification. During the PCR amplification step, 12 PCR cycles were performed, with a combined annealing and extension step for 10 s, to minimize the large DNA fragments [ChIP-seq] 1x 108 cells were crosslinked for 11 min at room temperature using 0.1% formaldehyde solution. Chromatin preparation and immunoprecipitation by BRG1 antibody (abcam, ab110641) were performed as previously described (soboleva et al., 2017). 40 μg chromatin and 5 μg BRG1 antibody  were used per ChIP assay. 1% chromatin was used as input for library construction. [ChIP-seq] The sequencing libraries were prepared using NEBNext Ultra II DNA library prep kit for Illumina following the kit manual