GSM9430277: GD21, Amitrole 25mg/kg bw/day, 53_20-24; Rattus norvegicus; RNA-Seq
Testis were dissected and frozen in liquid nitrogen. Samples were stored at –80 ˚C until further analysis. Total RNA isolation was performed using a RNeasy Micro Kit following manufacturer's protocol with the DNase step included (Qiagen, Germany). RNA quantity, quality, and purity was determined by a combination of a Nano-drop-1000 spectrophotometer (ND-1000, Fisher Scientific) and an Agilent 2100 Bioanalyzer System (Agilent Technologies, CA, USA). Samples with RNA integrity (RIN)-score > 6.8 were used. RNA samples were diluted to 25 ng/µL, and stored at -80 ˚C. The sequencing libraries were built by tagmentation using 50 ng of ds cDNA with the Illumina Nextera XT Kit (Illumina, #FC-131-1024) following the manufacturer's recommendations. Library strategy: Bulk RNA Barcoding and sequencing (BRB-seq)