Targeted BCR IG seq of human: PBMC COVID-19
BcR IG gene rearrangements were amplified on genomic DNA isolated from peripheral blood mononuclear cells (PBMCs). PCR amplification of IGHV-IGHD-IGHJ gene rearrangements was performed using IGHV subgroup specific primers annealing to the leader sequence and IGHJ gene primers. Library construction and next generation sequencing (NGS) were performed based on NEBNext Ultra II DNA Library Prep Kit for Illumina protocol instructions.