GSM8943086: CD8+T cells, control vector, rep1; Mus musculus; RNA-Seq
Total RNA was extracted using TRIzol reagent (Invitrogen) and conserved in RNase-free tubes by lipid nitrogen quick freezing. The total RNA (10-1000 ng) was applied to construct cDNA libraries using Whole RNA-seq Lib Prep kit for illumina (ABclonal, RK20303) or mRNA-seq Lib Prep Kit for illumina (ABclonal, RK20302) following the manufacturer's instructions.