GSM9788188: PHH cells POLA 3; Homo sapiens; RNA-Seq
RNA was extracted using the RNEasy® Plus mini kit (QIAGEN Sciences) according to manufacturer's instructions. The resulting RNA was treated with DNase (QIAGEN Sciences) and quality checked with an Agilent Bioanalyzer. mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. cDNA was then synthesised from this mRNA. The library was checked with Qubit and RT PCR for quantification and bioanalyzer for size distribution detection.