Metagenome of Soil Associated with Zea mays
The 16S library was prepared via two-step PCR 49 and was obtained via amplification of the 16S rRNA V4 region 48. The V4 region was amplified via the following primers: 16S Amplicon PCR Forward Primer (5'-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG-3') and 16S Amplicon PCR Reverse Primer (5'-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC-3')50. The ITS1 region was amplified via the newly developed primers ITS1FI2, 50-GAACCWGCGGARGGATCA-30, and ITS2 51. All libraries were prepared following the two-step PCR Illumina protocol and subsequently sequenced on an Illumina MiSeq instrument (Illumina, San Diego, CA, USA) using 2  251 paired-end reads.