DM_2 (CRX2028242)
Total RNA was extracted using the mirVana miRNA Isolation Kit (Ambion) following the manufacturer's protocol. RNA quality was assessed by the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The samples with RNA Integrity Number (RIN) >= 7 were subjected to the subsequent analysis. The cDNA libraries were constructed using TruSeq Stranded mRNA LTSample Prep Kit (Illumina, San Diego, CA, USA) and sequenced on the Illumina sequencing platform (HiSeqTM 2500 or Illumina HiSeq X Ten) and 150bp paired-end reads were generated. The transcriptome sequencing and analysis were conducted by OE biotech Co., Ltd. (Shanghai, China).