GSM8830589: IPF295_DMSO; Homo sapiens; RNA-Seq
Lung slices were homogenized in TRIzol™ Reagent (15596026, Invitrogen, Waltham, MA, USA) and treated with chloroform to extract RNA. The resulting aqueous phase was transferred to a clean tube, and an equal volume of 70% ethanol was added. The mixture was then loaded onto a column for RNA extraction and purification using the RNeasy Mini Kit (74104, QIAGEN, Hilden, Germany), following the manufacturer's protocol. RNA concentration was measured using a NanoDrop™ OneC Microvolume UV-Vis Spectrophotometer (840274200, Thermo Scientific, Waltham, MA, USA). RNA samples were subsequently submitted to the Genomics Core at the Van Andel Institute for bulk RNA sequencing on an Illumina NovaSeq 6000. RNA libraries for RNA-seq were prepared using by the Genomics Core at the Van Andel Institute using  total RNA