Comparative genomic analysis of Bacillus subtilis G01
Genomic DNA of B. subtilis G01 was extracted using the Bacterial DNA kit (Omega Co.) according to the manufacturers protocols. The experimental process is performed in accordance with the standard protocol provided by Oxford Nanopore Technologies (ONT). The extracted DNA quality was evaluated using Qubit 2.0 Flurometer (Life Technologies, Carlsbad, CA, USA) and Nanodrop (Thermo Fisher Scientific, Wilmington, USA) accordingly. After the samples were qualified, they were sequenced based on the Nanopore sequencing technology platform according to standard protocols. The Canu v1.5 software with default parameters was used to assemble the filtered subreads. Finally, the second-generation data were used by Pilon software to further correct the errors of the assembled genome to obtain a more accurate genome.