GSM9757062: Model group, rep2; Rattus norvegicus; RNA-Seq
Total RNA was extracted from the tissue; the extracted products were analyzed using an Agilent 4150 TapeStation (Agilent Technologies, Inc., Santa Clara, CA, USA) to assess their size distribution and integrity. mRNA was enriched from total RNA using Oligo(dT) magnetic beads to remove rRNA and other non‑coding RNAs. The mRNA was fragmented, reverse‑transcribed into cDNA, and ligated with adapters. After PCR amplification, the library was purified with magnetic beads, quantified, normalized, pooled, and converted into DNA nanoballs (DNBs) before sequencing.