GSM8896238: hPSC-CMs(WTC-11), Day30 cultured in standard RPMI/B27, bio rep 2; Homo sapiens; RNA-Seq
Total RNA was extracted from Trizol-collected samples by tri-phasic extraction followed by purification and concentration with Zymo Direct-zol MiniPrep Plus columns (Zymo – R2072) per kit instructions (including on-column DNAse treatment) Sequencing libraries were constructed from 500 ng of total RNA per sample using the Takara SMARTer Stranded Total RNA Kit – HI Mammalian