Spatial transcriptomics data from the lungNENomics cohort of lung neuroendocrine tumours
Spatial transcriptomics data from the lungNENomics cohort of lung neuroendocrine tumours
Formalin-fixed, paraffin-embedded (FFPE) tumour tissues were collected at diagnosis during surgical resection from contributing centres. Patients provided informed consent for tissue collection and its use in histopathological and molecular analyses (including somatic and germline whole-genome sequencing), as well as for the collection of de-identified clinical data. This study was approved by the International Agency for Research on Cancer Ethics Committee (project number 19-07). Each sample was placed on a 10x Genomics Visium slide followed by deparaffinisation, H&E staining and decrosslinking steps, according to 10x Genomics guidelines. Human probes targeting approximately 18,000 genes were hybridised overnight on the slides and captured on each spot after ligation between the LHS and RHS probes. Libraries were produced for each sample following 10x Genomics protocols.