16S rRNA (V3V4) amplicon sequencing of Sorghum bicolor at zero hour (before sowing) from saline field
Total genomic DNA was extracted from soil samples using the Qiagen DNeasy PowerSoil Pro Kit.The V3V4 hypervariable region of the 16S rRNA gene was amplified using universal primers 341F (5-CCTACGGGNGGCWGCAG-3) and 805R (5-GACTACHVGGGTATCTAATCC-3). Amplicons were generated using PCR, purified, using magnetic bead purification. Equal amounts of PCR amplicons from each sample were pooled, end-repaired, A-tailed, and ligated with Illumina adapters, followed by paired-end sequencing on an Illumina platform.