Potato stolon, BN1-1 (CRX2088871)
For RNA-seq experiment, we collected fresh stolons of N0, N1 and N2, with three biological replicates per treatment. RNA extraction and transcriptome sequencing were completed by OE Biotech Co., Ltd. (Shanghai, China). Total RNA was isolated from the samples using TRIzol reagent (Invitrogen, CA, USA) according to the manufacturer's instructions. Total RNA concentration, purity, and integrity were detected by NanoDrop 2000 spectrophotometer (Thermo Scientific, USA), Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), respectively. The qualified total RNA was used to construct the library by VAHTS Universal V6 RNA-seq Library Prep Kit. And the llumina Novaseq 6000 platform was used for sequencing analysis.