Illumina sequencing of soil bacteria, sample FH22
DNA was extracted from a 5 g subsample of soil sample of each plot using the PowerMax Soil DNA Isolation Kit (MoBio Laboratories, Inc.). Soil bacterial community composition, we amplified the target gene of 16S rRNA with primers 515F and 806R. The target region 16S rRNA gene was sequenced by Novogene Company (United Kingdom) using 2  250 bp paired-read sequencing approach on an Illumina NovaSeq platform.