GSM9433754: S2-HAGRHL2.Grh_450uM_R2; Drosophila melanogaster; ATAC-seq
200,000 cells were spun down for 3min at 600g at 4˚C, washed with 1X PBS, and resuspended in 100μL ATAC lysis buffer. Lysed cells were centrifuged for 10min at 600g at 4˚C. The supernatant was replaced with 47.5μL TD buffer and 2.5μL Tn5 enzyme (Illumina) and incubated at 37˚C for exactly 30min. DNA was purified with the MinElute Reaction Cleanup kit (Qiagen). Purified DNA was PCR amplified with IDT for Illumina Nextera DNA Unique Dual Indices in NEBNext Hi-Fi 2x PCR Master Mix (72˚C for 5min, 98˚C for 30s, followed by 12 cycles of 98˚C for 10s, 63˚C for 30s, and 72˚C for 1min). Libraries were purified with Axygen paramagentic beads (Thermo Fisher Scientific) at a ratio of 1.2x and eluted in 10 mM Tris.