GSM8655577: Knee OA synovium_18; Homo sapiens; RNA-Seq
Synivial tissues were collected surgically. Immediately after collection, they were removed from bone and adipose tissues using surgical scissors, crushed with a bead homogenizer, and dissolved in TRI Reagent (Molecular Research Center).Total RNA was extracted using Direct-zol RNA MicroPrep Kit (ZYMO RESEARCH). Library was prepared using the NEBNext Ultra II RNA Library Prep Kit (Illumina) according to each specimen protocol.