RNASeq_R6_EpiLC
ESCs were harvested and directly plated onto fibronectin plates in freshly prepared EpiLC medium consisting of N2B27 (homemade) constituted of 10  L/mL knock-out serum replacement, 20 ng/mL Activin A, and 12 ng/mL Fibroblast growth factor 2 (bFGF). After 72 hours, RNA was extracted using RNeasy Kit (QIAGEN), library prep was done using Illumina Stranded mRNAPrep, Ligation (Illumina). Libraries were sequenced on a NovaSeq X Plus (Illumina).