GSM9645722: C8-D1A, 37℃ incubated, 8h, rep2; Mus musculus; miRNA-Seq
Exosomal miRNA was extracted by the MagZol (Magen) according to the manufacturer's protocol. The quantity and integrity of Exosomal RNA yield was assessed by using the Qubit®2.0 (Invitvogen,USA) and Agilent 2200 TapeStation (Agilent Technologies, USA) separately. Briefly, RNAs were ligated with 3' RNA adapter, and followed by 5' adapter ligation. Subsequently, the adapter-ligated RNAs were subjected to RT-PCR and amplified with a low-cycle. Then the PCR products were size selected by PAGE gel according to instructions of NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Illumina, USA). The purified library products were evaluated using the Agilent 2200 TapeStation.