GSM8943989: SCC-25, RARG KO, 1μM Retinoic Acid, 6h, rep3; Homo sapiens; RNA-Seq
Total RNA was collected from the cells with 350μL Lysis Buffer RLT from RNeasy Plus Mini Kit (Qiagen, Cat. No. 74134) with 1:100 addition of β-mercaptoethanol, followed by gDNA removal and extraction according to the kit protocol. Samples with RNA integrity number ≥ 9 were selected for cDNA library preparation with NEB Ultra II Directional RNA Library Prep (plus Poly A isolation module) according to the manufacturer's instructions.