GSM8510832: shNC_3; Homo sapiens; RNA-Seq
Total RNA was extracted using the mirVana miRNA Isolation Kit (Ambion) following the manufacturer's protocol. The libraries were constructed using TruSeq Stranded mRNA LTSample Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer' s instructions. RNA libraries were prepared for sequencing using standard Illumina protocols