bacterial sequence of soil around mangrove tree
ibraries were prepared by PCR using fusion primers containing an 8-nt index sequence and the gene-specific primer sequence (see Table 3). Libraries were purified with Agencourt AMPure XP beads. Double-stranded PCR products were heat-denatured and circularized by the splint oligo sequence, thus forming single-stranded circular DNA (ssCir DNA). DNA nanoballs (DNBs) consisting of 300+ copies were formed from ssCir DNA molecules by rolling-cycle replication. DNBs were loaded into patterned nanoarrays using high-intensity DNA nanochip technique and sequenced through combinatorial Probe-Anchor Synthesis (cPAS) (Fehlmann et al., 2016) on MGI DNBSEQ-G400 in 2 300 bp mode.