ddRAD-Seq of Physocarpus capitatus: Stensvold 8382 (ALA)
DNA was extracted from leaf tissue harvested from ALA herbarium specimen using Qiagen DNeasy Plant Pro kit. Genomic DNA was digested with restriction enzymes Eco-R1 and Mse1, barcode adapters were ligated to fragments, then fragments were PCR amplified. Libraries were pooled and shipped to Harvard Bauer Core for pre-sequencing QC, size selection (200-400 bp), and sequencing.