GSM8943440: CastratedMouseProstate_C57BL6_rep1; Mus musculus; RNA-Seq
The 10x barcoding and complementary DNA (cDNA) synthesis were performed using 10x chromium 3′ scRNA-seq  V3.1 chemistry according to the manufacturer's instructions. Briefly, an appropriate volume of single-cell suspension/enzyme mix, barcoded beads, and partitioning oil were loaded in distinct lanes on the chip and processed in a 10x chromium controller instrument. The 10x GemCode Technology uses a droplet-based method to partition thousands of cells to generate nanoliter-scale Gel Bead-In-Emulsions (GEMs). Inside each GEM, cDNA generated from an individual cell shares a common 10x barcode. The prepared GEMs were incubated in a thermomixer to produce cDNA. Recovered cDNA was cleaned up and amplified by polymerase chain reaction to generate enough quantity for library construction. The size and yield were evaluated using a high-sensitivity bioanalyzer assay chip (Agilent). A set of recommended primers (P5, P7, and R2) in the manufacturer's standard protocol were added to prepare the Illumina-ready sequencing library. The final library was evaluated for size and yield using a high-sensitivity bioanalyzer assay chip (Agilent). The final libraries were sequenced with the NovaSeq 6000 according to recommended specifications.