DNA-seq of human fecal microbiome
Post-extraction DNA was prepared using the rapid barcoding 96 kit version 14 (SQK-RBK114.96). The final pool of barcoded samples was added to a GridION flow cell (R10.4.1). GridION reads were base-called using the Super Accuracy Analysis protocol, achieving an estimated accuracy of 99.5%. Adaptor sequences were subsequently trimmed using Porechop v0.2.4. Quality filtering was performed with Chopper v0.5.0, retaining reads with a minimum length of 300 base pairs and a sufficient PHRED score.