GSM8634483: 172 (VPCI 218/P/20)_rep1; Pichia kudriavzevii; RNA-Seq
Total RNA was purified using the TRI reagent (Sigma). Quality of RNA was assessed on a Bioanalyzer using the RNA6000 Nanochip (Agilent). mRNA was enriched using oligo(dT) beads (NEB) and subsequently, double-stranded cDNA libraries were generated by using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB) according to the manufacturer's instructions.