IRR-RSPO2
The UCLA Technology Center for Genomics & Bioinformatics performed the RNA-seq analysis using established methods for library construction and sequencing. Single-cell libraries were constructed following manufacturer's protocol using the Chromium GEM-X single-cell 3 GEM (10X Genomics) kit targeting 10K cells. Different adaptors were used for multiplexing samples in one lane. Sequencing was performed on Illumina NovaSeqX+ for a PE 2x150 run. Data quality check was done on Illumina SAV. Data de-multiplexing was performed with Illumina BCL Convert v4.2.7 software to R1=28nt and R2=90nt.