Mutant2-Heading stage-1 (CRX2063217; CRA031301)
Total RNA was extracted from spike tissues collected at three developmental stages (0, 5, and 10 days post-heading) using the RNAprep Pure Plant Kit (TIANGEN, Beijing, China) following the manufacturer's instructions. RNA quality and integrity were assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies, USA). Libraries were prepared using the Illumina TruSeq Stranded mRNA Library Prep Kit and sequenced on an Illumina NovaSeq 4000 platform to generate 150 bp paired-end reads.