Whole Exome Sequencing of adult man
Obtain DNA samples that have passed the quality test and use an ultrasonic crusher for random fragmentation. The fragmented DNA fragments are then subjected to end repair, A-tailing, adapter ligation, purification and pre-amplification, quantification, exon capture, and PCR enrichment to construct a high-throughput sequencing library. The completed library is initially quantified and diluted using Qubit3.0, followed by detection of the library's insert size and nucleic acid concentration. This ensures the accuracy of the sequencing concentration and data output.