Single-round XL RaPID selection against hPCSK9
DNA libraries were subjected to a single-round XL RaPID selection. Libraries were made with one internal methionine substitution per peptide sequence to allow for reprogramming with photo-reactive amino acids. Double indexed libraries (Nextera XT indices) were prepared with the DNA recovered after each round and sequenced on a Illumina NovaSeq X Plus using a 10B flowcell and 200 cycle reads. P1(TAATACGACTCACTATAGGGTTAACTTTAAGAAGGAGATATACATATGTATGCTAATTTTGGTCGGAATTGTATTAGGGCTGGCGCTCAGCCTGGCGCTAGCGCTTGGAGCCATCCTCAGTTTGAAAAAGGTTCTGGTTCTGGTTCTTAGGACGGGGGGCGGAAA).P6(TAATACGACTCACTATAGGGTTAACTTTAAGAAGGAGATATACATATGGGTTTGCATGATTATTATGCTTGGTATTTGTGCGGCGCTCAGCCTGGCGCTAGCGCTTGGAGCCATCCTCAGTTTGAAAAAGGTTCTGGTTCTGGTTCTTAGGACGGGGGGCGGAAA).