GSM8721634: D8_ATRA; Mus musculus; RNA-Seq
Differentiated cells after 4, 8 or 16 days of treatment were disociated (Worthington Papain Dissociation System) and resuspended to the required concentration based on the 10X Genomics protocol requirements. The Chromium instrument and Single cell 3' Reagent Kit v3 (PN-1000128; 10X Genomics) was used to prepare individually barcoded single-cell RNA-Seq whole transcriptome libraries following the manufacturer's protocol. The target number of cells was 8000 per condition. Libraries were sequenced on Illumina Novaseq 6000 using paired-end 150 cycles v3 kits (Read 1: 28 cycles; Index Read 1 (i7): 10 cycles; Read 2: 90 cycles).