GSM8459891: NOMO-1_rep1; Homo sapiens; RNA-Seq
Total RNA was extracted with Tri Reagent per the manufacturer's instructions (AM9738 – Thermo Fisher), followed by DNase treatment (AM2238 – Thermo Fisher). RNA quality was assessed by Agilent Tapestation 2200 with all samples achieving a RIN >9.5. We used 3 µg of total RNA for RT-qPCR, or RNA-Seq. The Illumina TruSeq Stranded mRNA kit was used for mRNA-seq library preparation, with one round of RiboZero Gold for ribo-depletion. Pooled libraries were sequenced using the Illumina HiSeq 4000 instrument at Edinburgh Genomics (http://genomics.ed.ac.uk/) generating 2 × 150 bp paired-end reads.