16s rDNA of milk sample210161: 1-2 days after calving
Using the NEXTFLEX Rapid DNA Seq Kit to build a library of purified PCR products: (1) linker linkage; (2) Use magnetic beads to screen and remove adapter self connecting fragments; (3) Enrichment of library templates using PCR amplification; (4) Magnetic beads were used to recover PCR products and obtain the final library. Sequencing using Illumina Nextseq2000 platform