GSM9537133: 24 months AF cells MDL-800 treated 30; Mus musculus; RNA-Seq
NP and AF tissues were isolated under microscope and placed in RNA later immediately. Samples were stored in -80 until extraction. RNA was isolated using RNA-easy mini kit (Qiagen). Libraries for whole transcriptome RNA sequencing were prepared using the Stranded Total RNAseq with Ribo-zero Plus kit (Illumina, San Diego, CA) as per manufacturer's instructions starting with an input of 50 ng of RNA and 14 cycles of final PCR amplification. Library size was assessed using the 4200 TapeStation and the DNA D5000 ScreenTape assay (Agilent, Santa Clara, CA). Library concentration was determined using the Qubit Fluorometer 2.0 (ThermoFisher Scientific, Waltham, MA).