GSM8463083: R3_Response_RNA; Homo sapiens; RNA-Seq
Total RNA was extracted using Trizol Take a certain amount of RNA sample, prepare a reaction system, and use the RNase H method kit to remove rRNA. React at an appropriate temperature for a certain period of time to fragment RNA Add a pre prepared one strand synthesis reaction system to the interrupted RNA and set up a reaction program to synthesize one strand cDNA; Prepare a double stranded synthesis reaction system (including dUTP) and set up a reaction program to synthesize double stranded cDNA. Repair the double stranded cDNA end and add an A base at the 3 'end to prepare a linker connection reaction system. Set up a reaction program to connect the linker to the cDNA and amplify the product. After denaturing the PCR product into a single stranded form, a cyclization reaction system is prepared and a reaction program is set up to obtain a single stranded circular product. The linear DNA molecules that have not been cyclized are digested.