RNA-Seq of mus musculus: adult male brain
The concentration of the single-cell suspension was adjusted to 700-1200 cells/L. Single-cell RNA sequencing libraries were constructed using the Chromium Next GEM Single Cell 3 Kit v3.1 (PN-1000268, 10x Genomics, USA), according to the instructions provided by the manufacturer. Libraries were sequenced on an Illumina NovaSeq 6000 platform with paired-end 150 bp reads. Further sequencing and bioinformatics analysis were performed by OE Biotech Co., Ltd. (China).