18S rDNA amplicon of 2023sprB07L2 (CRX2174960)
Genomic DNA was isolated through lysozyme-SDS-phenol/chloroform protocols and amplified with uniquely indexed primers targeting the 16S and 18S ribosomal RNA (rRNA) gene regions.The V3-V4 hypervariable region of the 18S rRNA gene was amplified with primer pair 528F/706R,High-resolution sequencing was performed on an Illumina Nova6000 platform (250-bp paired-end)