Spleen_parenchyma_Sow (CRX2062556; CRA031277)
For Illumina sequencing, 2 ug of total RNA for each sample was used to construct sequencing libraries using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, E7530L) following the manufacturer's instructions. The prepared libraries were then subjected to transcriptome sequencing on an Illumina NovaSeq platform using paired-end 150 nt reads (Benagen, Wuhan, China).