WGBS of THP-1: mut rep2
A total of 100 ng of genomic DNA was first spiked with 0.5 ng of lambda DNA and then was fragmented to an average size of 200300 bp using Covaris S220. Next, the fragmented DNA was subjected to bisulfite conversion using the EZ DNA Methylation-GoldTM Kit (Zymo Research). Library preparation was then performed by Novogene Corporation (Beijing, China), followed by paired-end sequencing on an Illumina platform (Illumina, CA, USA).