GSM9500758: Human ileal enteroid line 1004 HME_POOL; Homo sapiens; RNA-Seq
RNA was isolated with the Qiagen RNeasy Micro kit according to the manufacturer's protocol. Libraries were created from the cDNA by first blunt ending the fragments, attaching an adenosine to the 3′ end, and finally ligating unique adapters to the ends for PCR amplification. Paired end sequencing (2 × 150 bp) was performed on an Illumina NovaSeq 6000  for a depth of 30 million paired end reads (a total of 60 million reads) per RNA sample.