RNA-Seq of Pelodiscus sinensis liver (Control, replicate 1) (CRX2124036)
Total RNA was extracted from liver tissue using Trizol reagent (Invitrogen, Carlsbad, CA, USA). Subsequently, mRNA was enriched using Oligo (dT) magnetic beads and fragmented in Frag/Prime buffer. The RNA fragments were reverse-transcribed into double-stranded cDNA. Sequencing libraries were then constructed following procedures of purification, end repair, adapter ligation, and PCR amplification. Qualified library products were sequenced on an Illumina NovaSeq platform.