WGS of Broccoli
The extracted DNA from 7-day-old broccoli was fragmented into smaller pieces. The fragmented DNA ends were repaired to create blunt ends by filling in overhangs and phosphorylating the 5' ends. An adenine (A) nucleotide was then added to the 3' ends of the DNA fragments to prevent self-ligation. P5 and P7 adapters were ligated to the A-tailed DNA fragments. The adapter-ligated DNA fragments were amplified using PCR to enrich the library and incorporate sequencing-specific motifs. Finally, the prepared library was loaded onto a NovaSeq 6000 for sequencing.