GSM8488193: LNCaP cells, Promoter VP, siNT, rep2; Homo sapiens; OTHER
Cells were crosslinked with 10% paraformaldehyde for 10 min and quenched with glycine. Chromatin was serially digested and ligated with DpnII followed by NlaIII. Libraries were produced using a two-step PCR stragegy. The first PCR is an inverse PCR using enhancer viewpoint specific primers. The second PCR incorporates the indexed Illumina sequencing adaptors. Library quality was assessed by Agilent TapeStation.