GSM9860299: Unwounded skin tissue 4; Mus musculus; RNA-Seq
Total RNA from skin tissue and cells were isolated using a RNeasy Mini Kit (Qiagen, Valencia, California). Total 100 ng Library preparation was carried out using the KAPA mRNA HyperPrep kit (KAPA BIOSYSTEMS, KK8580) according to the manufacturer's protocols. Library quality was assessed using an Agilent 4200 TapeStation (Agilent, CA, US). Paired-end sequencing was performed on a NextSeq 2000 platform (Illumina).