GSM9860461: LS8 cells, Control, H3K27Ac (ChIP); Mus musculus; ChIP-Seq
Cells were fixed with 1% formaldehyde for the crosslinking was stopped by adding glycine. Cells were subsequently lysed, and the isolated chromatin was sheared in the kit-provided lysis buffer via sonication (30 s ON, 30 s OFF, 30 cycles) using a Bioruptor®® Pico (Diagenode). ChIP-seq librarieswere prepared using the TruSeq ChIP Library PreparationKit (Illumina, San Diego, CA, USA). The prepared library was examined via a bioanalyzer and Qubit (Thermo Fisher Scientific) to test library quality and quantity, respectively.