Human Skin Metagenome_S289
Tape discs were transferred into Lysing Matrix E tubes (MP Biomedicals) with 100L of ATL Buffer (QIAGEN) and 1.2% Triton X-100. Briefly, extracted DNA (27L) was used to construct libraries using NEBNext Ultra II FS (New England Biolabs, Cat. No. E7805 protocol) with use of barcode adaptors (NEBNext Multiplex Oligos for Illumina Cat. No. E6440). Custom index primers with 14 PCR cycles were used for enrichment of DNA libraries, which were quantified using the Agilent DNA 1000 Kit on an Agilent 2100 Bioanalyzer (Agilent Technologies). Samples were normalized and pooled, followed by paired-end sequencing (2150bp reads) on the Illumina HiSeq X platform.