Differential gene expression analysis of in vitro expanded CD4 T cells responding to HHV-6B and HCMV epitopes
Differential gene expression analysis of in vitro expanded CD4 T cells responding to HHV-6B and HCMV epitopes
Epitope-specific CD4 T cells were isolated from PBMC cultured in vitro in presence of epitope peptides (10 ug/mL) for ~12-15 days, with IL2 addition to the medium at days 4 and 8. Isolation was performed using DRB1*03:01 tetramers carrying the epitope peptides and flow cytometry-based cell sorting.  Total RNA was extracted from tetramer-sorted cells using RNeasy kit (Qiagen). Libraries were prepared from 250 ng of RNA with RQN>7.2, using the Illumina TruSeq v2 platform and following manufacturer's instructions.