iSeq 100 sequencing of Human: MHC HLA Class I
The DNA extraction was performed using the QIAamp DNA Blood Mini Kit, and the DNA was quantified using Qubit Fluorometric Quantification. Amplification was carried out on the VeritiPro Thermal Cycler, 96-well, using the GenDx MX6-1 amplification kit. The library was prepared according to the manufacturer's protocol. Sequencing was performed on the iSeq 100 Sequencing System by Illumina. The data were processed in NGSengine version 3.2.1.35905 with IMGT version 3.57.0.