GSM9140051: Enzalutamide_24hr_1; Homo sapiens; RNA-Seq
RNA was purified using the Qiagen All-Prep kit following manufacturer's instructions (Qiagen). Cell lysates were harvested by the direct addition of lysis buffer to cell culture plates and collected using a cell scraper whist on ice. RNA samples were stored at -80°C. 1μg total RNA from each replicate condition was used as input for library preparation using the TruSeq RNA Library Prep kit and following the manufacturer's protocol (Illumina). Final libraries were pooled to obtain equimolar pools each at 10nM concentration. Sample pools were submitted for paired-end sequencing (2x150bp) on Illumina HiSeq-4000, targeting ~30 million reads per sample.