GSM9892199: HUCCT1 PBX1 CUT&Tag replicate 1; Homo sapiens; ChIP-Seq
CUT&Tag was performed on HUCCT1 and RBE cell samples. Cells or nuclei were bound to ConA magnetic beads. Exact starting cell number and fixation status were not recorded in the available report. CUT&Tag libraries were reported to be prepared using the NovoNGS CUT&Tag 4.0 High-Sensitivity Kit (for Illumina; Novoprotein). A rabbit polyclonal anti-PBX1 antibody was used (Proteintech, 18204-1-AP; RRID: AB_10951860). The standard workflow included primary antibody incubation at 4C overnight, secondary antibody incubation at room temperature for 1 h, pA-Tn5-mediated tagmentation, PCR amplification, and library quality control. Libraries were sequenced on an Illumina NovaSeq platform with 150-bp paired-end reads. Exact antibody amount, input cell number, fixation status, and PCR cycle count were unknown.