RNAseq for Arabidopsis seedlings: control_AT_r2
Total RNA was isolated from the 40-50 mg of Arabidopsis seedlings using a combination of TRIzol/chloroform extraction and kit method (PureLink RNA Mini Kit, Thermo #12183018A). Extracted RNA quantity was check on Qubit fluorometer (Thermo #Q33238) using RNA HS assay kit (Thermo #Q32851) following manufactures protocol. RIN values of the samples were obtained using Tapestation 4150. Paired-end sequencing libraries were prepared using SMART-Seq Library Prep Kit (R40047) as per the manufacturers protocol.