GSM8829636: OVCAR-8, Untreated, 2; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol reagent, following the manufacturer's protocol. RNA quality was assessed using the Agilent 2100 Bioanalyzer, and concentrations were determined using ThermoFisher's NanoDrop. Stranded RNA-seq libraries were constructed using the NEBNext poly(A) mRNA isolation kit, followed by the IDT XGen Broad Range RNA Library Prep Kit, according to the manufacturer's protocol. 1 µg of RNA was used for library construction, and libraries were indexed for multiplexed sequencing.