GSM9894163: Uninfected parental Hoxb8-derived macrophages, biological replicate 3; Mus musculus; RNA-Seq
RNA was extracted using TRIzol. Total RNA from the aqueous phase was purified using the Zymo RNA Clean & Concentrator kit. Barcoded RNA-seq libraries were generated with the NEBNext Ultra II Directional RNA Library Prep Kit after ribosomal RNA depletion using the QIAseq FastSelect pan-bacterial and H/M/R kits. Libraries were sequenced on an Illumina NovaSeq 6000 S4 flow cell.