CT26 vehicle 3 (CRX2096955)
Total RNA was extracted from tumor tissues using Magzol reagent. RNA integrity was assessed with an Agilent 2100 Bioanalyzer, and purity and concentration were measured using a NanoDrop 2000 spectrophotometer. Poly(A)-enriched libraries were prepared with the Directional RNA Library Prep Kit and sequenced on an Illumina HiSeq 4000 platform.