GSM8605818: PBMC, low, 243; Homo sapiens; RNA-Seq
RNA was isolated from 1*10^6 PBMCs using RNeasy Mini kit (Qiagen) including DNAse I digestion according to manufacturer's instructions. RNA bulk sequencing was performed by Single Cell Discoveries (Utrecht, The Netherlands) with a sequencing depth of 20 million reads/sample. Library preparation was performed according to the CEL-seq2 protocol. Sequencing was performed on a Nextseq500 (Illumina).