Transcriptome of Camellia oleifera seed kernels after 12 h heat stress (T12)
Total RNA was extracted from Camellia oleifera seed kernels subjected to heat stress at 35C for 0 h, 12 h, and 24 h using the TRIzol reagent. RNA integrity and purity were assessed with an Agilent 2100 Bioanalyzer and NanoDrop spectrophotometer. mRNA was enriched using oligo(dT) magnetic beads, fragmented, and reverse-transcribed into cDNA. The cDNA library was constructed using the Illumina TruSeq RNA Sample Preparation Kit and sequenced on an Illumina NovaSeq 6000 platform to generate paired-end reads of 150 bp.