GSM8721175: iPax3 re-isolated, N3OE, rep3; Mus musculus; RNA-Seq
RNA isolation: samples with low input of RNA (in vivo cells, re-isolated donor-derived satellite cells), RNA was extracted using Buffer RLT (QIAGEN) and nucleic acid beads. For library contruction: samples with low input (in vivo cells, re-isolated donor-derived satellite cells). The cDNA was synthesized using the NEBNext Single Cell/Low Input cDNA Synthesis & Amplification kit (New England Biolabs) and the libraries were generated with the Nextera XT DNA Library Preparation Kit (Illumina).