GSM9059414: three week old, rosette, ZT6, jaz4-1_1; Arabidopsis thaliana; RNA-Seq
RNA was extracted from Col-0 and jaz4-1 plants using RNeasy plant mini kit (Qiagen, Valencia, CA) For library preparation and transcriptome sequencing, 1 μg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample. Library quality was assessed on the Agilent Bioanalyzer 2100 system. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using PE Cluster Kit cBot-HS (Illumina) according to the manufacturer's instructions. After cluster generation, the library preparations were sequenced on an Illumina platform and paired-end reads were generated. Illumina sequencing and quality control were performed at Novogene Corporation Inc. (Sacramento, CA, USA).