GSM8943463: PBMC, CCC10 clinical form; Homo sapiens; RNA-Seq
RNA was extracted from single cells using the BD™ Single-Cell Multiplexing Kit (BD Biosciences), followed by cDNA synthesis and library preparation with the BD Rhapsody Express Single-Cell Analysis System. The BD Rhapsody cDNA kit (cat# 633663) and BD Target Amplification kit (cat# 633664) were used according to the manufacturer's protocols cDNA libraries were constructed using the BD Rhapsody Express Single-Cell Analysis System (BD Biosciences), following the manufacturer's instructions. The BD Rhapsody cDNA kit (cat# 633663) and BD Target Amplification kit (cat# 633664) were used for reverse transcription, cDNA synthesis, and amplification. Libraries incorporated sample barcodes and Unique Molecular Identifiers (UMIs) during cDNA synthesis to enable single-cell and molecule-level resolution.