Genome Sequencing of Large, Multigenerational CEPH/Utah Families sample 80090
Unique dual-indexed, single stranded libraries were prepared from 500-750ng of genomic DNA using the Illumina DNA PCR-Free Library Prep, tagmentation protocol. A double-sided size selection was performed post ligation according to protocol. Size selection was performed post ligation and modified (1.4x single sided bead clean up) to optimize the final insert size.  After samples were pooled prior to sequencing, a 0.8x single sided bead clean-up was performed to remove smaller fragments and further optimize the final insert size.: ILMN Tagmentation Library Prep 1.0