GSM8862477: 2802F; Mus musculus; ChIP-Seq
Nuclei were isolated and sonicated using a Covaris ultrasonicator (Active motif). Sheared chromatin was incubated with 5µg antibody per sample overnight at 4°C on a rotator, followed by immunoprecipitation using magnetic beads. After washing, DNA was purified using a DNA Clean & Concentrator Kit (Zymo). ChIP-Seq with NEBNext Ultra II DNA Library Prep Kit