WGS HiFi sequnecing of Actinidia melliana RY-41
Total genomic DNA were extracted using a modified CTAB method (Allen et al., 2006) and evaluated using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) and Qubit fluorometer instrument (Thermo Fisher Scientific, MA, USA). A standard SMRTbell library was prepared with 50 g of gDNA by using the SMRTbell Express Template Prep Kit 2.0, according to the manufacturers instructions. SMRTbell libraries were then sequenced on a PacBio Sequel II system (Pacific Biosciences, CA, USA).