GSM8829374: naïve KI T CD4 cells-2; Mus musculus; RNA-Seq
Total RNA was prepared using Rneasy Mini Kit (GIAGEN). 15ng total RNA was used for the isolation of poly(A)+ RNA by NEBNext Poly(A) mRNA magnetic isolation kit (New England Biolbs) following manufactures's protocols. Libraries were prepared by TaKaRa SmartSeq Standed Kit following manufacture's protocols.