PkNF-YA7a-OE callus, input control (CRX2065969; CRA031376)
ChIP-seq was performed on PkNF-YA7a-overexpressing (OE) and empty vector (EV) callus. Chromatin was cross-linked, fragmented to 200-500 bp, and immunoprecipitated with anti-Flag magnetic beads. Protein-DNA complexes were eluted, cross-links reversed, and DNA quality verified by agarose gel electrophoresis before library preparation and sequencing.