GSM8862593: chimpanzee NPC, 10X gene expression library, reaction 1; Pan troglodytes; RNA-Seq
At D14, infected cells (BFP+) were sorted by FACS and 4 scRNA-seq reactions were prepared for each cell line using the Chromium Next GEM Single Cell 3' Reagent Kit v3.1 (Dual Index) with Feature Barcoding technology for CRISPR Screening (10X Genomics, cat #PN-1000268, #PN-1000269, #PN-1000262, #PN-1000120, #PN-1000127, #PN-1000215, #PN-1000242). CRISPR gRNA libraries and gene expression libraries were prepared from each reaction using the Chromium Next GEM Single Cell 3' Reagent Kit v3.1 (Dual Index) with Feature Barcoding technology for CRISPR Screening (10X Genomics, cat #PN-1000268, #PN-1000269, #PN-1000262, #PN-1000120, #PN-1000127, #PN-1000215, #PN-1000242).