GSM8459745: H3K9me2,MT,Rep2,CUT&RUN; Mus musculus; OTHER
Cells were washed twice and immobilized on concanavalin A-coated magnetic beads. Cells were then incubated overnight at 4°C with primary antibody in antibody binding buffer. After washing with dig-wash, cells were incubated with pA-MNase complex in wash buffer for 1 hour at 4°C. Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina. Five nanograms (5 ng) of DNA was used for library preparation, and the libraries were amplified using 13 cycles on the thermocycler. The final libraries were cleaned up using AMPureXP beads, validated using the Agilent High Sensitivity DNA kit, and the concentrations were measured by Qubit.