PVAT_RNA-Seq 2
mRNA was enriched from total RNA using oligo(dT) magnetic beads. Libraries were constructed with the VAHTS Universal V6 RNA-seq Library Prep Kit (Vazyme), including RNA fragmentation, first/second-strand cDNA synthesis (random priming), end repair/A-tailing, adaptor ligation, bead-based purification/size selection, and PCR amplification.