DNA sequencing of Symbiopectobacterium purcellii MEX: long-read
To obtain a pure strain, a single colony from one of the two insect heads was triple cloned on PWG. Bacterial DNA was extracted using the blood and tissue kit (Qiagen) preceded by a pretreatment for Gram-negative bacteriaas suggested in the manufacturers instructionsbefore sequencing with Illumina HiSeq 4000 and PacBio Sequel I at the University of California, Berkeley Vincent J. Coates Genomics Sequencing Laboratory (California Institute for Quantitative Biosciences; QB3).