GSM8722572: 5xFAD, Microglia, PKR KO, rep1; Mus musculus; RNA-Seq
Total RNA from tissues was extracted using TRIzol reagent (Invitrogen), 1 ug of total RNA was used for the construction of  sequencing library. Total RNAs were processed with RiboMinus kit (Human/Mouse Module, Invitrogen) to deplete most ribosomal RNAs (ribo- RNA). The ribo- RNA libraries for sequencing were prepared with illumina TruSeq® Stranded Total RNA Library Prep Gold (#20020598) kit. All libraries were sequenced with NovaSeq 6000 system at Sequanta Technologies. FastQC was applied for raw read qualities assessment.