RNAseq of prostate cancer cell
Cells were harvested and total RNA was extracted using mirVana miRNA Isolation Kit (Ambion, Cat. No. AM1561) according to the manufacturers protocol. RNA quality was examined using a NanoDrop spectrophotometer (Thermo Scientific, Massachusetts, USA). Library was constructed using TruSeq Stranded mRNA LTSample Prep Kit  (IlluminaSan Diego, California, USA) according to the manufacturers instructions.