OSCA_29_A cell line
DNA samples that passed QA/QC (260/280 ratio > 1.8; >200 ng total DNA) were pooled and sequenced on three NovaSeq 6000 S4 run using TruSeq Nano DNA prep and paired-end sequencing mode. The samples have 1545M-2220M (Canfam4) and 1553M-1670M (hg38)  pass filter reads, with Q30 above 90%. The samples were mapped and variants were called using Dragen. Percent total mapping against reference genome CanFam4 and hg38 is about 99% and 98% respectively, and uniquely mapped reads are above 84%. Library complexity (i.e. percentage of non-duplicate reads) was determined by measuring the percentage of unique fragments in the mapped reads using MarkDuplicate utility. Percent duplicated reads are between 5%-10% (Canfam4) and 5-7% (hg38). Coverage statistics were also measured using Dragen. The mapped sequencing depth coverage (after alignment and marking duplicates) was between 80x-116x (Canfam4) and 63x-74x (hg38). The mean insert size for these samples was between 552-622 bases (Canfam4) and 540 -569 bases (hg38). More than 93% of genome have the coverage above 20x.