WGBS-NSN-rep2 (CRX2069146)
30 mouse oocytes (per sample) were lyzed in 20 ul of lysis buffer consisting of 1x M-Digestion Buffer and 1 mg/ml Proteinase K (Zymo Research) for 20 min at 50oC. Bisulfite conversion and library preparation were performed using the Pico Methyl-Seq Library Prep Kit (Zymo Research) after minor modifications. In the amplification with PrepAmp Primer step, PrepAmp primer was diluted from 40 uM to 20 uM. In the library amplification step, 10 cycles were used. The purified libraries were quantified by Qubit 1X dsDNA HS Assay Kit and qPCR, analyzed by a Fragment Analyzer System to confirm successful amplification and quality, and sequenced using Novaseq 6000 Sequencing System.