GSM9644797: mCherry cells, early-HSPCs_TLN; Danio rerio; RNA-Seq
Kidney marrows, TLN, thymus and intestines were dissected from the adult fish and dissociated according to eastablished protocols for each tissues. For intestine, the tissue was digested with collagenase. For kidney, TLN and thymus, tissue was triturated gently to release the hematopoietic cells. The mCherry expressing cells were isolated from each tissue using FACS. Libraries were prepared according to manufacturer's instructions of the kits. For all adult tissues, the Chromium Next GEM Single Cell 3' Kit v4 was used.