GSM9571699: BMDM, NT,24hrs, rep1; Mus musculus; ATAC-seq
50,000  BMDMs per sample were lysed to collect nuclei and treated with Tn5 transposase (Illumina) for 30 min at 37°C with gentle agitation. The DNA was isolated with DNA Clean & Concentrator Kit (Zymo) 598 and PCR amplified and barcoded with NEBNext High-Fidelity PCR Mix (New England Biolabs) and unique dual indexes (Illumina). The ATAC-seq library amplification was confirmed by real-time PCR, and additional barcoding PCR cycles were added as necessary while avoiding overamplification. Amplified ATAC-seq libraries were purified with DNA Clean & Concentrator Kit (Zymo). The purified libraries were quantified with Kapa Library Quant Kit (KAPA Biosystems) and quality assessed on a 4200 TapeStation System (Agilent). The libraries were pooled based on molar concentrations and 605 sequenced on a HiSeq 4000 platform single end, 75 bp).