16S rDNA sequencing of Arabidopsis root microbiome: CMRt1
High quality DNA was extracted using FastDNATM SPIN Kit. 16S rDNA gene was amplified using primers targeting V5-V7 region. Libraries were prepared using NEB Next UltraTM II FS DNA PCR-free Library Prep Kit. A pool of libraries were sequenced on MiSeq v2 at Novogene.