GSM8463258: L144160; Mus musculus; RNA-Seq
The assembloids or organoids were dissociated to single cells using TrypLE 1x for 10min at 37°C. The cells were resuspended in PBS with 0.04% BSA, 0.2U/μL RNAse inhibitor (Merck, #3335399001) and 10μM ROCK inhibitor (Ri) (Y-27632, Merck/Sigma, #Y0503) in BSA-coated tubes. The cells were filtered with 100 μm strainer, cell suspensions (1 000 – 20 000) were loaded into Chromium Next GEM ChipG and processed as specified in manufacturer's instructions. Single-cell RNA-Seq libraries were prepared using Single Cell 3ʹ v3.1 Gel Bead kit (10X Genomics) as per manufacturer's instructions