COI mini-barcode amplicon sequencing of commercial sea urchin product SUP-6
COI mini-barcode amplicon sequencing of commercial sea urchin products. A 267 bp PCR product containing a 227 bp COI mini-barcode was generated using forward primer GCACCAGACATGGCTTTCCC and reverse primer AGACATTCCTGGTGTCCGCA. Indexed paired-end libraries were sequenced on an Illumina NovaSeq 6000. Barcode and primer sequences were removed, and overlapping paired reads were merged using FLASH. Only merged FASTQ reads are submitted; therefore the submitted library layout is SINGLE.