GSM8459871: [Hi-C] oeCTCFmut_IAA_rep1; Mus musculus; Hi-C
Crosslinked cells were treated with 0.1% SDS FA lysis buffer and 1% SDS FA lysis buffer.The genome was digested with enzyme HaeIII into fragments with blunt-ends, which were treated with adenine and ligated with bridge linker containing biotin at 16 ℃ for 4 hours. The unligated linker were digested with DNA exonuclease. Next, the cells were treated with SDS and proteinase K to digest proteins, and the DNA was purified using phenol-chloroform extraction with ethanol precipitation. The DNA was fragmented into 300 bp on average by sonication and the biotin-labeled DNA fragments were pulled down with streptavidin coated M280 beads for DNA library construction followed by sequencing.