Caenorhabditis sp. 59 RNAseq
After pools of worms were flash-frozen in liquid nitrogen, total RNA was extracted using a trizol+chloroform protocol and RNAeasy spin column cleanup including DNAse treatment. We then used the Illumina Stranded mRNA Prep kit to generate mRNA, convert it to cDNA, ligate adapters from the IDT for Illumina RNA UD indices set B, and amplify the library. The library was sequenced on NextSeq500 with a MidOutput 2x150 v2.5 configuration at NYUs GenCore facility.