GSM8830126: monocyte_sia_donor9; Homo sapiens; RNA-Seq
Total RNA was isolated from primary human monocytes using a RNeasy Micro Kit (Qiagen), following the manufacturer's instructions. cDNA libraries were generated from a total of 50 ng RNA according to the Oxford Nanopore Technologies (Oxford Nanopore Technologies Ltd, Oxford, UK) protocol “cDNA-PCR Barcoding Sequencing”60 with 18 cycles of PCR (4.5 min elongation time). ONT adapters were ligated to the amplified cDNA library.