GSM9610952: GFP_pos_CTR_plasmid_rep1; Mus musculus; RNA-Seq
A minimum of 50,000 cells, cultured in high serum-containing medium and at low density, were collected per sample for RNA extraction (Quick-RNA, Zymogen, ZR1050) following the manufacturer's protocol. Reverse transcription was performed using the SuperScript III Reverse Transcriptase (Invitrogen, 18080-093) following the manufacturer's guidelines. cDNA was generated following the SMART-Seq2 protocol. The RNA-Seq libraries were fragmented using Tn5 transposases with adaptors and amplified using PCR.