GSM9262057: V-AM; Mus musculus; RNA-Seq
After WT mice were euthanized, BALF was collected by flushing the airways 10 times with 10 ml of sterile PBS (37 °C) containing 0.5% FBS (HyClone™, Cytiva) and 2 mM EDTA using a 1 ml syringe. Cells were pelleted, subjected to red blood lysis, and total RNA was extracted using REzol reagent (Protech) followed by purification with the Direct-zol RNA Microprep Kits (Zymo), according to the manufacturer's instructions. The purified RNA was used for the preparation of the sequencing library by TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA) following the manufacturer's recommendations.