sequences of Microbial
Next generation sequencing library preparations were constructed following the  manufacturers protocol. 200g genomic DNA was randomly fragmented by Covaris to an  average size of 300-350bp. The fragments were treated with End Prep Enzyme Mix for end  repairing, 5 Phosphorylation and 3 adenylated, to add adaptors to both ends. Size selection of  Adaptor-ligated DNA was then performed by DNA Cleanup beads. Each sample was then  amplified by PCR for 8 cycles using P5 and P7 primers, with both primers carrying sequences  which can anneal with flowcell to perform bridge PCR and P7 primer carrying a six-base index  allowing for multiplexing.