Low-dose CFS mouse duodenum sample 3
Genomic DNA was extracted from duodenal and cecal content samples collected from BALB/c mice administered low-dose or high-dose Latilactobacillus sakei cell-free supernatant or vehicle control by gastric gavage for 7 days. The V3V4 hypervariable regions of the bacterial 16S rRNA gene were amplified using universal primers 341F (CCTACGGGNGGCWGCAG) and 805R (GACTACHVGGGTATCTAATCC). Library preparation was performed using a two-step PCR approach with PrimeSTAR GXL DNA Polymerase and Nextera XT indexing primers. Libraries were purified using Agencourt AMPure XP beads and sequenced on an Illumina NovaSeq 6000 platform using paired-end 2  150 bp chemistry.