GSM8708847: sorted CD4+ T cells from pediatric SLE donor, SLE12; Homo sapiens; RNA-Seq
Sorted live CD4+ T cells were resuspended in PBS supplemented with 0.2% non-acetylated BSA (Miltenyi) and viability was determined using trypan blue staining and measured on Countess FLII (Thermo-Fisher). 50,000 cells were loaded for capture on 10x Chromium system using the V3 single cell reagent kit for 3 prime RNA sequencing (10x Thechnologies). After capture and lysis, cDNAs from the cells were amplified as per 10x Chromium's recommendations (10x Thechnologies. The amplified cDNAs were loaded on a single lane of NovaSeq 6000 for a depth of 50,000 reads per cell.