16S of Pseudocheirus peregrinus (ringtail possum, RTP);  fecal microbiome
The V3-V4 region of the bacterial 16S ribosomal gene was amplified using the primer pair 341F- 5-CCTACGGGNGGCWGCAG-3, and 805R - 5-GACTACHVGGGTATCTAATCC-3. According to the standard protocols, Library normalisation and pooling were performed, and sequencing was completed on an Illumina MiSeq platform (2x300 bp) inhouse at La Trobe University (Melbourne, Australia). All samples were sequenced in one run to avoid potential run -to-run variation.