PacBio HiFi 70-15
SMRTbell target size libraries were constructed for sequencing according to PacBios standard protocol (Pacific Biosciences, CA, USA) using 15kb preparation solutions. The main steps for library preparation are: (1) gDNA shearing, (2) DNA damage repairend repair and A-tailing, (3) ligation with hairpin adapters from the SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences), (4) nuclease treatment of SMRTbell library with SMRTbell Enzyme Cleanup Kit, (5) size selection, and (6) binding to polymerase.