GSM8862472: donor 2, 1st_veh (Protein); Homo sapiens; OTHER
cells were washed once in PBS, incubated in cell staining buffer (Biolegend, Cat#420201) and Human TruStain FcX Blocking (Biolegend, Cat#422301) reagent for 10 min, then incubated for 30 min at 4°C in TotalSeq antibody cocktail (supplementary table 1). Cells were then washed 3 times in cell staining buffer, resuspended in PBS and filtered through a 40um Flowmi Cell Strainer (Bel-Art, Cat#H13680-0040). Cells were counted and diluted to 1000cells/ul for appropriate input into the 10X Chromium chip. Samples were then processed using the Chromium Next GEM Single Cell 5' Kit v2 (10x Genomics, Cat#1000263) to generate DNA sequencing library for Illumina and the libraries were sequenced on a NovaSeq6000 with S4-200 kit (Illumina), following 10x Genomics recommendations for loading concentration and read length.