GSM9058804: BasalCells, Ctrl, E16.5, Rep1; Mus musculus; RNA-Seq
10,000 Epcam+α6+ double positive basal cells were sorted from dorsal skin of embryos into FACS buffer (5% FBS in HBSS buffer, w/o Ca2+/Mg2+), and sit on ice for downstream processing Library preparation was performed using Chromium Single Cell 3' Reagent Kit v3 from 10x Genomics following manufacturer's instruction, targeting for 5000 cells per sample.