GSM8655925: B cells, B220+, BCR_B18383_Set1, BCR; Mus musculus; OTHER
Single cells after sorting were encapsulated in Gel beads in Emulsion (GEM) using the 10X Controller with a Chromium Next GEM Single Cell 5' Kit v3, Chromium Single Cell Mouse BCR Amplification Kit, and Chromium 5' Feature Barcode Kit. library preparation using Chromium GEM-X Single Cell 5' Reagent Kits v3 (10X Genomics) according to the manufacturer's protocol. Briefly, after gel-beads in emulsion (GEMs) generation, RT-PCR, cDNA amplification, fragmentation, end repair, A-tailing and sample indexing were performed. CITE-seq