GSM9107829: GFP2; Homo sapiens; RNA-Seq
Total RNA was extracted using ureLink® RNA Mini Kit (Invitrogen™, # 12183018A). The quality and quantity of isolated RNA was checked using NanoDrop™ spectrophotometer (Thermofisher scientific), Qubit™ 4 Fluorometer (Invitrogen, #Q33226) and Agilent 2100 Bioanalyzer System. RNA was extracted and used to prepare sequencing libraries with the TruSeq Stranded Total RNA Library Prep Kit (Illumina, #20020597) on a Biomek i7 automated workstation (Beckman Coulter).