direct RNA ONT sequencing of P. falciparum Dd2 Gametocytes stage V day 16 in vitro polyadenylated
Libraries were prepared from 150 ng RNA, which was in vitro polyadenylated E. coli Poly(A) Polymerase (New England Biolabs) for 75 sec at 37C, using the SQK-RNA002 kit and sequenced on a MinION flow cell (R9.4.1). Only reads above 200 nt were sequenced. Raw fast5 reads generated with guppy-4.2.2.