small RNA-Seq of Homo Sapiens: adult plasma
miRNeasy Serum/Plasma Kit (catalog number: 217184, Qiagen) was used to extract total RNA in plasma. QIAseq miRNA Library Kit (catalog no. 331505, Qiagen) and QIAseq miRNA NGS 96 Index IL (catalog no. 331565, Qiagen) were used to generate small RNA libraries, which were further sequenced at 75-bp single end.