Expression of miRNA in Response to Aflatoxin B1
Total RNA was isolated by RNAzol RT extraction, DNase-treated. RNA concentration and quality was assessed by spectrophotometry and each sample was quantified by RiboGreen Assay on the 2100 Bioanalyzer. Indexed libraries were constructed with the TruSeq smRNA library preparation kit and size selected. Libraries were multiplexed and sequenced on the HiSeq2500 to produce 50-bp single-end reads