GSM8862441: MEFs, RSL3, 2 hours  rep2; Mus musculus; RNA-Seq
Total RNA from HT1080 cells and lung homogenates were extracted using AG RNAex Pro Reagent (Accurate Biotechnology, #AG21102) according to the manufacturer's instructions. RNAs were reverse-transcribed using Evo M-MLV RT Premix for qPCR (Accurate Biotechnology, #AG11706). Libraries were constructed using the TruSeq Stranded mRNA LT Sample Prep kit (Illumina, San Diego, CA, USA). We then sequenced these libraries on the Illumina sequencing platform ( Illumina HiSeq X Ten).