GSM8893672: CD45+ Immune Cells in the Mouse Brain – H2030 Brain Metastasis (BrM) Treated with Ibudilast; Mus musculus; RNA-Seq
CD45+ cells were isolated by FACS FACS-purified CD45⁺ cells were processed for library preparation using the Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1 (10x Genomics, PN-1000121), following the manufacturer's instructions. The protocol included GEM generation, barcoding, GEM-RT clean-up, cDNA amplification, and library construction. The sequencing was conducted on an Illumina NextSeq 550 instrument equipped with v2.5 reagent kits, employing a PairEnd run type of 28 bp R1 and 56 bp R2. Primary data processing, including image analysis, per-cycle basecalling, and quality score assignment, was carried out using Real Time Analysis software (RTA v2, Illumina). Additionally, the conversion of BCL files to FASTQ format was executed with bcl2fastq2 within the Local Run Manager RNAfusion Analysis Module v2 (Illumina).