GSM9610373: control-1; Mus musculus; RNA-Seq
Strand-specific RNA-seq libraries were constructed using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs). Briefly, mRNA was enriched from total RNA, fragmented, and reverse-transcribed into double-stranded cDNA. After end repair, adapter ligation, and PCR amplification, libraries were sequenced on an Illumina NovaSeq 6000 platform (150 bp paired-end).