16S rRNA V4 amplicon sequencing of tomato bulk soil microbiome
16S rRNA V4 region amplified with primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3'); PCR with Phusion HF Master Mix, 30 cycles at 98C/50C/72C; library prep with NEBNext Ultra II FS DNA Library Prep Kit; 2x250 bp paired-end sequencing by Novogene