GSM8655609: circDNA2 KD_2, RNA-Seq; Homo sapiens; RNA-Seq
RNA was harvested using Trizol reagent.RNA quality and quantity was measured by using NanoDrop and RNA Integrity was determined by Agilent 2100 bioanalyzer 1-2 µg of total RNA was selected from each sample for sequencing library construction. The total RNA was first enriched for mRNA using the NEB Next® Poly(A) mRNA Magnetic Isolation Module, and then the library was constructed using the KAPA Stranded RNA-Seq Library Prep Kit (Roche) based on the dUTP strand-specific library construction method.