RNA-Seq of female Oedaleus asiaticu: 5th-instar 3-day-oldbiological replicate 1
Total RNA extracted using Eastep Super Total RNA Extraction Kit following manufacturer's instructions. cDNA library constructed using Hieff NGS Ultima Dual-mode mRNA Library Prep Kit for Illumina. Library quality validated by Qsep-400 (Q30  85%). Library concentration quantified using Qubit dsDNA HS Assay Kit. Sequencing performed on Illumina HiSeq 4000 platform using sequencing by synthesis (SBS) technology. Raw reads were filtered to remove adapters, primers, and low-quality sequences, generating high-quality clean reads.