GSM8634677: Taf4 in Taf13 expressing; Mus musculus; OTHER
Cut and Tag experiments were carried out on 200 000 live cells using CUT&Tag-IT™ Assay Kit (Active motif, Catalog No. 53165 for TBP and Taf4, and No. 53160 for Xpb) according to the manufacture's manual. 10 000 drosophila nuclei (or 5%) (Active motif, Catalog No. 53173 for TBP and Taf4 and No. 53168 for XPB) were added as spikes to each sample at the same time as Concanavalin A Beads. Libraries were prepared according to the Active motif CUT&Tag-IT™ Assay Kit manufacture's manual (Catalog No. 53165 and No. 53160) using Nextera™-Compatible Multiplex Primers (Active motif, Catalog No. 53155).