GSM9537750: MCF10CA1a, RNA-seq, biological replicate 3; Homo sapiens; RNA-Seq
Total RNA was isolated from cells using Trizol (Life Technologies) and purified using the Direct-zol RNA kit (Zymo Research, Irvine, CA, USA: R2050). RNA quality and quantity were assessed using the RNA 6000 Nano Kit with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). RNA quantity was further assessed using a Nanodrop2000 (Thermo Scientific, Lafayette, CO) and Qubit HS RNA assay (Thermo Fisher Scientific). Total RNA was depleted of ribosomal RNA (New England Biolabs, NEB #7400), reverse transcribed using a random hexamer strategy, and strand-specific adapters were added following the NEBNext Ultra II RNA-seq library prep kit (New England Biolabs, E7770).