Head-cKO-2 (CRX1980640)
Total RNA was extracted from bone samples. Poly(A)+ mRNA was enriched, fragmented, and reverse transcribed into cDNA. Libraries were prepared by end repair, adapter ligation, and PCR amplification. Sequencing was performed on the Illumina NovaSeq 6000 platform with paired-end 150 bp reads. The RNA-seq reads were aligned to the mouse genome (GRCm39) using HISAT2, and transcript quantification was performed with StringTie.