GSM8827780: Psph-WT in MM biol rep 2; Pseudomonas savastanoi pv. phaseolicola 1448A; RNA-Seq
1mL of stationary phase (OD600= 2-2.5) bacterial culture, 3mL of mid-log-phase culture (WT and ∆vimR strains) cultured in MM for 6 hours, and 1mL of PAO1-WT and PA3565 overexpressing strains in logarithmic phase(OD600=0.6-1.0) were collected and centrifuged (12,000rpm, 4℃). Subsequent RNA extraction and purification were performed using RNeasy mini kit (Qiagen) and treated with DNaseI (NEB). After removing rRNA using the MICROBExpress kit (Ambion), cDNA Libraries were constructed using mRNA according to NEBNext® UltraTM II RNA Library Prep Kit protocol (Illumina). It was then sequenced using the HiSeq 2000 system (Illumina) platform. All RNA-seq experiments were performed for two biological replicates.