ChIP-seq of Homo sapiens ESCC KYSE150 cells, input
ESCC cells were crosslinked with 37% formaldehyde and terminated with 2.5 M glycine. Then the cell nuclei were extracted and chromatin was fragmented using a contact sonication instrument. 6 L of FOXP4 antibody (Proteintech Cat# 16772-1-AP, China) or an equivalent amount of IgG was incubated with the fragmented chromatin at 4 C overnight. And the protein A/G magnetic beads were added to a tube for chromatin immunoprecipitation. Finally, the ChIP Elution Buffer were added to the immunoprecipitated samples to elute the chromatin for the collection of purified DNA. The purified DNA were ligated with specific adapters for ChIP-seq, followed by sequencing on the Illumina NovaSeq 6000 sequencer using the PE150 strategy.