GSM8942239: whole mouse embryonic heads, 12ss, control, biological replicate 1; Mus musculus; RNA-Seq
Whole embryonic heads were cut caudal to the first pharyngeal arch, snap frozen in a dry ice/MeOH slurry, and stored at -80C until extracted using the Rneasy kit (Qiagen 74004) Library preparation was completed by the CCHMC Genomics Sequencing Facility where RNA was polyA-selected, reverse-transcribed using Illumina's TruSeq stranded mRNA library preparation kit. Amplification was accomplished with 15 cycles of PCR.