SP80-3280__C405__Culm__12 months old__6h
Next-generation sequencing workflow, based on a custom RNA AmpliSeq panel, was designed for sequencing 708 transcripts related to sugar-signalling pathways. Transcript sequences encoding proteins associated with trehalose metabolism (TPS class I, TPS class II, TPP, and TRE) were identified and characterized by Oliveira et al. (2022), while sequences related to TORC1, SnRK1, HXK, HXK-Like were sourced from Oliveira et al. (2024). Additionally, sequences of enzymes involved in sucrose and starch metabolism [sucrose phosphate synthase (SPS), UDP-glucose pyrophosphorylase (UGPase), and ADP-glucose pyrophosphorylase (AGPase)], and targets of TOR [autophagy-related proteins ATG1 and ATG13, and ribosomal proteins from the large and small subunits (RPL and RPS] and SnRK1 [basic leucine zipper transcription factor 11 (bZIP11)] were also identified following the method described above and used in our RNA panel.