DNBSEQ-G400 paired end sequencing of SAMD00886165
Library was performed according to the Chromium Next GEM Single Cell 3' Reagent Kits v3.1, instruction (10x Genomics). To perform target enrichment of Calhm1, Calhm3, Tas2r105 and Scn2a cDNA, we developed a custom targeted strategy employing 3 rounds of linear amplification and PCR in a nested manner. The amplified cDNA products were fragmented using Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (10x Genomics) and were selected with SPRIselect reagent for subsequent library construction.