GSM8655508: Ecto_CBC_GBC; Homo sapiens; RNA-Seq
The hPSC cells were infected with lentivirus and sorted based on fluorescence protein expression. The sorted cells were recovered for one week, followed by induced differentiation towards ectoderm lineage. Cells were washed with PBS and digested to single cell with Accutase (Stem Cell Tech), then washed again with PBS and resuspended to the concentration suggested by 10X Genomics library preparation instruction. The library was prepared according to 10X Genomics single cell 3' v2 protocol.