16S - Non-sterile soil treated with water - Day 9 Survey - Rep.2 (CRX2072389)
DNA was extracted from soil samples, strictly following the manufacturer's instructions. The V4-V5 region of the bacterial 16S ribosomal RNA gene was amplified by PCR using primers. The purified PCR products were quantified using Qubit 3.0. The 24 PCR amplicons with different barcodes were mixed equally. The mixed DNA products were used to construct an Illumina Pair-End library according to the Illumina genomic DNA library preparation process. Subsequently, sequencing was performed on an NGS platform using a standard paired-end sequencing program.