QQ Fosmid from deep sea sediment of Arabian Sea set II
High-molecular-weight metagenomic DNA was extracted from deep-sea sediment samples collected from the Arabian Sea and Bay of Bengal and cloned into the pCC1FOS fosmid vector to construct metagenomic fosmid libraries. Fosmid clones exhibiting quorum quenching activity were selected for sequencing. Genomic DNA from positive fosmid clones was used to prepare Illumina paired-end sequencing libraries using the Illumina DNA Prep Kit (Illumina, USA) according to the manufacturer's instructions. Sequencing was performed on the Illumina NextSeq 2000 platform to generate 2  150 bp paired-end reads. Raw reads were quality-checked using FastQC, and adapter trimming and quality filtering were performed using fastp. High-quality reads were de novo assembled using SPAdes with multiple k-mer sizes (21, 33, 55, and 77). The assembled contigs were used for the identification and characterization of quorum quenching genes from deep-sea sediment metagenomic fosmid clones.