TCR-repertoire analysis of peptide-specific T-cell responses after vaccination with wtSARS-CoV-2 nucleocapsid peptide LLLLDRLLNQLESKMS in two healthy volunteers.
T cell receptor libraries were prepared using SMARTer Human TCR a/b Profiling Kit v2 (Takara Bio). Purification and Selection of amplified libraries was performed using NucleoMag NGS Clean-up and Size Select Beads according to manufacturers instructions. gammadelta T cell receptor libraries were prepared with the same kit but with a modified protocol including custom gamma/delta primers by the commercial service provider MedGenome Inc. Sequencing was performed with a MiSeq system (Illumina) using the MiSeq Reagent Kit v3 600-cycle.