RNA-seq-sibling6 (CRX2086056)
To investigate the role of actl6a in protonephros development and ciliary stability in zebrafish, we used CRISPR/Cas9 to knockout actl6a and constructed a mutant strain. The sibling and mutant embryos were cleaved and the tissue of pronephric duct was isolated.  We then perfomed gene expression profiling analysis using data obtain from RNA-seq of 10 different pronephric duct per group between sibling and actl6a-/-. Comparative gene expression profiling analysis of RNA-seq data for pronephric duct of 3 dpf sibling and actl6a-/-.