GSM8830227: Melanoma tumor cells, donor 1; Homo sapiens; RNA-Seq
Melanoma tumors were collected post-operatively from two patients who consented and signed IRB. To obtain single-cell suspensions, samples were washed in PBS and cut into small pieces (4-5 mm3) followed by dissociation using the Miltenyi human tumor dissociation kit according to manufacturer instructions. Red blood cells were depleted using ACK lysis buffer for 3 minutes. Dead cells were removed, if needed, using the Milyenty dead cell separator. Single cell encapsulation and library preparation were performed using the inDrop platform followed by sequencing on an Illumina NextSeq. Sequencing reads were de-multiplexed, aligned and counted using a custom pipeline as described previously (Baron et al 2020) to produce a raw count matrix for each tumor.