RNA-Seq
Wild-type and lgp2-/- zebrafish embryos at 3 dpf were collected separately. Total RNA was extracted using the TRIzol reagent method. RNA integrity and purity were assessed by agarose gel electrophoresis and a ultramicro spectrophotometer. For each group, the three highest-quality RNA samples were selected, designated as AB1, AB2, AB3 (wild-type) and DLX1, DLX2, DLX3 (lgp2-/-), and used for library construction according to the manufacturer's instructions of the Hieff NGS Ultima Dual-mode mRNA Library Prep Kit (Yeasen, Shanghai, China). After construction, library quality was assessed for fragment size distribution and concentration using an Agilent 2100 Bioanalyzer with the DNA 1000 assay kit (Agilent Technologies, Palo Alto, USA). Qualified libraries were sent to Guangzhou Genedenovo Biotechnology Co., Ltd. for high-throughput sequencing.