RNA-Seq of Cre-ERT2 Foxo1fl/fl mouse B-ALL + 4OHT
Total RNA was isolated from 1x106 cells using the RNeasy kit (Qiagen, Hilden, Germany). RNA integrity (RIN) was analyzed using the Agilent 2100 bioanalyzer (Agilent, Santa Clarita, USA). Library construction and RNA-sequencing was performed by Novogene Co., LTD (Beijing, China).