GSM9431785: hs_THP1_6h_Calcitriol_100nM_Anti-H3K27ac_ab4729_ChIPseq_rep2; Homo sapiens; ChIP-Seq
The cells were cross-linked with 2 mM disuccinimidyl glutarate for 40 minutes and 1% methanol-free formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sheared using a Diagenode Bioruptor sonicator. Precipitated and washed samples were purified using NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel). Indexed cDNA libraries were prepared from 1 to 10 ng of ChIP-DNA using an Ovation® Ultralow System V2 (Tecan)