ItaRoSie099
Mouse fecal samples were extracted using the DNeasy 96 PowerSoil Pro QIAcube HT (QIAGEN)51. Briefly, approximately 50-80 mg of each feces was added to a PowerBead ProPlate well along with 3 wells of water control. 800 L CD1 was added to each well. Stool was homogenized by bead beating: TissueLyser II (QIAGEN) as directed by the manufacturer's protocol. After centrifugations, samples were transferred to a 96-well plate compatible with the QIAcube HT system using the DNeasy Powerlyzer-Power soil kit. Manufacturer protocol was followed to isolate the DNA. The NanoDrop Eight Spectrophotometer was used to evaluate DNA concentration and quality, with three negative controls included on the plate (extraction blank).