Whole metagenome of sewage sludge
The genomic DNA was randomly sheared into short fragments. The obtainedfragments were end repaired, A-tailed and further ligated with Illumina adapter. Thefragments with adapters were PCR amplified, size selected, and purified. The library was checked with Qubit and real-time PCR for quantificationandbioanalyzer for size distribution detection. Quantified libraries will be pooledandsequenced on Illumina platforms, according to effective library concentration and data amount required