RNA-seq of COTS juvenile spines
Sequencing was performed using the Illumina NextSeq500 (NextSeq control software v4.2.0.112 / Real Time Analysis v2.11.3). The library pool was diluted and denatured according to the standard NextSeq protocol (Document # 15048776 v18) and sequenced to generate paired-end 149 bp reads using a 300 cycle NextSeq500/550 High Output reagent Kit v2.5 (illumina, 20024908). After sequencing, fastq files were generated using bcl2fastq2 (v2.20.0.422), and sequencing adapters were trimmed. The first cycle of each insert read was also trimmed due to expected low diversity.