GSM9078819: J30OE1; Homo sapiens; RNA-Seq
Total RNA was extracted from the cells using TRIzol reagent according the manufacturer's instructions. The RNA quality was determined by 5300 Bioanalyser (Agilent) and quantified using the ND-2000(NanoDrop Technologies). Only high-quality RNA sample(OD 260/280 =1.8~2.2, OD 260/230 ≥ 2.0, RQN ≥ 6.5, 28S:18S ≥1.0,>1μg) was used to construct sequencing library.