WT1 (CRX2072138)
We conducted RNA-seq analysis using three biological replicates for both WT and pnkp-/- zebrafish. For each replicate, 30 fishes each replicate (5 dpf larvae) was dissected. The tail tissue was used for genotyping, and the heads of homozygote mutant were selected for RNA extraction. Total RNA of WT and pnkp-/- zebrafish heads was isolated and subsequently used for cDNA synthesis. Illumina sequencing, with library construction from Novogene (China), was used for the RNA-seq process.