GSM8721200: CD19_mock_4; Mus musculus; RNA-Seq
cerebrum (whole brain minus the cerebellum) was dissected out and the tissue was digested and processed using the Multi Tissue Dissociation Kit (Miltenyi Biotech) followed by debris removal (Miltenyi Biotech).  Mouse F4/80+ cell selection was performed using F4/80+ selection kit (Biolegend). The viability of cells after the isolation process was typically over 90%. F4/80+ cells were then processed with the Nextera XT Library Prep Kit (Illumina) to generate sequencing libraries following the manufacturer's instructions.