Nanopore WGS of the HeLa-JVM cell line
DNA was extracted from 2.5x10^6 HeLa-JVM cells using a Monarch HMW kit (NEB, T3050L). A nanopore DNA sequencing library was prepared by the Garvan Sequencing Platform (Australia) using 3μg input DNA sheared to ~25kbp using a g-TUBE (Covaris, 520079) and an SQK-LSK114 ligation sequencing kit, then sequenced using a PromethION (Oxford Nanopore Technologies) flow cell (FLO-PRO114M, R10.4.1 chemistry).