GSM8721702: IgG_lef_2; Mus musculus; RNA-Seq
Total RNA was extracted with TriZol, and RNA degradation and potential contamination were determined with 1% agarose gels. Library construction was performed with a NEBNext® UltraTM RNA Library Prep Kit Illumina® (NEB, USA) with 3 µg RNA per sample following the manufacturer's recommendations. Library quality was evaluated with  DNBSEQ-T7 system.