GSM9076313: 51T-2D cells, Palbociclib, day3, rep2; Homo sapiens; RNA-Seq
Total RNA was extracted from cells using TRIzol®. 200 µL Chloroform was added per 1000 µL TRIzol. For phase separation, samples were centrifuged, and the aqueous phase was used to precipitate the RNA with 800 µL isopropanol. Two washing steps with 75 % ethanol were performed before the RNA was resuspended in nuclease-free water. 400 ng RNA was used for sequencing. mRNA library preparation (poly A enrichment) and reverse transcription into cDNA followed by QC at Novogene