PacBio ITS amplicon sequencing of Lepanthes root endophytes: root sample
The entire fungal internal transcribed spacer (fITS) ITS1-5.8S-ITS2 region was PCR amplified with ITS1-F_KYO2 (Toju et al. 2012) and ITS4 (White et al. 1990) and and ITS4-Tul  (Taylor & McCormick 2008). We designed forward and reversed primers to be fused with PacBio barcodes and applied combinatorial dual-indexed barcoding system in the library preparation.