Participant ZH5125HIV Dab-seq library (DNA encoded antibodies part) Sequencing run 1
HIV Dab-seq libraries were generated with a custom droplet microfluidic workflow that mimics the commercial Tapestri workflow from Mission Bio. It consists of a cell encapsulation step in a first droplet to completley lyse the cells wit proteinase K. After heat inactivation, droplets are injected into a second device and merged with a multiplexed PCR mix to create single cell barcoded amplicons from a panel of genomic and target amplicons and DNA encoded antibodies. The panels targets 48 himan genomic regions, 10 HIV proviral regions and 165 antibodies (biolegend panel A).