JM_SC_3 (CRX703004)
Total RNA was extracted using Trizol reagent kit (Invitrogen, Carlsbad, CA,USA). RNA quality was assessed on an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA) and checked using RNase free agarose gel electrophoresis. mRNA was enriched by Oligo(dT) beads, then fragmented into short fragments and reverse transcripted into cDNA with random primers. Then the cDNA fragments were purified with QiaQuick PCR extraction kit(Qiagen, Venlo, The Netherlands), end repaired, A base added, and ligated to Illumina sequencing adapters. The ligation products were size selected by agarose gel electrophoresis, PCR amplified, and sequenced using Illumina Novaseq6000.