GSM8569530: C33ARB1_miR218; Homo sapiens; RNA-Seq
Total RNA was isolated using TRIzol (Invitrogen) reagent according to the manufacturer's protocol. mRNA was enriched from total RNA using oligo(dT) beads. After fragmentation, the first strand of cDNA was synthesized using random hexamer primers, followed by the second strand of cDNA synthesis using dTTP. Subsequent steps of end repair, A-tailing, adapter ligation, size selection, amplification, and purification were performed to obtain double-stranded cDNA libraries. Quantified libraries will be pooled and sequenced on Illumina platforms, according to effective library concentration and data amount.