Library collected from diabetic murine wound
DNA was enzymatically fragmented and adapters added using the NEB Ultra II FS kit (New England Biolabs), and ~50ng of fragmented adapted gDNA was used as a template for enrichment by PCR (16 cycles) for the transposon insertions using mariner-specific primer and Illumina P7 primers. Libraries were sequenced on both Illumina Nxtseq 2000 and Novaseq 6000 platforms before R1 reads were concatenated into this single file for further processing