GSM8996505: Valid_hESC_ChIP-H9_WT_H3K4me3-D14K4-1; Homo sapiens; ChIP-Seq
The Smart-seq2 method followed previously published studies. Briefly, approximately 10 -100 cells/sample were lysed, and cDNA was synthesized directly from cytoplasmic lysate. This full-length was amplified for 17 cycles, and libraries were prepared using the KAPA Hyper Prep kit. 3 biological replicates were perfomed. Sequenced on Illumina NovaSeq. ULI-NChIP-seq was performed using 200-500 cells/ sample, with two or three biological replicates per group. Isolated cells were washed with PBS/BSA, and the ULI-NChIP procedure was performed as previously described using 1μg of a histone H3K27me3 or H3K4me3 antibody per immunoprecipitation reaction77. Libraries were prepared using the KAPA Hyper Prep Kit. Sequenced on Illumina Novaseq.