MIG-seq of Pseudocrangonyx spp.
This library is made using MIG-seq which is a PCR-based procedure to construct reduced representation libraries, representing de novo single-nucleotide polymorphism discovery and its genotyping using NGS. Using multiplexed inter-simple sequence repeat primers, thousands of genome-wide regions were amplified effectively from a wide variety of genomes, without prior genetic information. See Suyama & Matsuki, 2015 Sci. Rep.; Suyama et al. 2022 Eco. Res. for the detail.