Targeted-seq of homo sapiens: LSP4
Genomic DNA was isolated from peripheral blood leukocytes using the QIAamp DNA Blood Mini Kit, following the manufacturers instructions (Qiagen). DNA was quantified using the Qubit 3.0 Fluorometric Quantitation (Thermo Fisher Scientific). An aliquot of 100 ng of genomic DNA was used to prepare the library using the OncoRisk Cancer Panel according to the protocol provided by Celemics. The libraries were quantified using the Qubit dsDNA HS Assay Kit (Life Technologies), then pooled and prepared for sequencing using the MiSeq Reagent Kit v3