Whole Shotgun metagenomic sequencing of Sink3-8 Ptrap biofilm
Genomic DNA was extracted using the DNeasy PowerSoil Kit (Qiagen) following the manufacturers protocol with modifications to enhance yield. DNA was eluted in 50 l of C6 buffer. DNA concentration and quality were assessed using a Qubit 4 Fluorometer with the 1X dsDNA HS Assay (Invitrogen). Extracted gDNA was stored at 20C until shipment at ambient temperature. Extracts were paired-end sequenced up to a read length of 150bp on Illumina NovaSeq platform, using a commercial provider (Azenta GeneWiz, UK).