GSM8723321: TypeII,QIAseq1-StepAmpliconLibraryKit,Illumina,NextSeq500,PCRcycles16,NextSeq1_S7; synthetic construct; OTHER
Plasmid preparation Illumina sequencing: QIAseq 1-Step Amplicon Library Kit (Qiagen), QIAseq FX DNA Library Kit (Qiagen), and xGen Prism DNA Library Prep Kit (Integrated DNA Technologies). PacBio sequencing: SMRTbell prep kit 3.0 (PacificBiosciences) and SMRTbell binding kit 2.0 (Pacific Biosciences). Nanopore sequencing : Native Barcoding Kit 96 V14 (Oxford Nanopore Technologies (ONT)) together with NEBNext® Ultra II End Repair / dA-tailing Module (New England Biolabs (NEB)), NEB Blunt/TA Ligase Master Mix (NEB), and NEBNext® Quick Ligation Module (NEB).