GSM9109264: Primary mouse hepatocytes, FD replicate #1; Mus musculus; RNA-Seq
RNA was extracted from primary hepatocyte samples using RNeasy Mini Kit (Qiagen #73404). RNA concentration and quality was determined by Qubit Bioanalyzer. Standard RNA-seq pipeline at Columbia Genome Center: poly-A pull-down to enrich mRNAs from total RNA samples (200ng-1µg per sample, RIN>8). STRPOLYA libraries were constructed using Illumina TruSeq RNA Preparation Kit according to the manufacturer's instructions.