GSM9430087: H3K18La_2; Oryctolagus cuniculus; OTHER
Genomic DNA was extracted following the CUT&Tag procedure using the Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro (Vazyme, TD904). Briefly, cells or nuclei were immobilized on concanavalin A–coated magnetic beads and incubated with primary and secondary antibodies. A protein A/G–Tn5 transposase complex was then applied to tagment chromatin at antibody-bound sites. DNA fragments were released by SDS treatment and purified using magnetic beads, followed by PCR amplification to generate sequencing libraries. Sequencing libraries were constructed using the Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro (Vazyme, TD904). Following H3K18la antibody-guided in situ tagmentation by protein A/G–Tn5 transposase, DNA fragments containing sequencing adapters were directly PCR-amplified using Illumina-compatible index primers. Amplified libraries were purified with magnetic beads and quality-checked prior to sequencing.