16S amplicon: Surface Microbiome of Calliblepharis jubata cultured on Ceramium sp. SSM Agar Rep 1
Microbial DNA was extracted using a Qiagen DNeasy PowerWater Kit and Zymo Clean & Concentrator-25 Kit following standard protocols. DNA concentrations were quantified using the Invitrogen Qubit DNA High Sensitivity Kit and DNA integrity assessed by visual inspection on 1% agarose gels. Sequencing of the 16S rRNA gene V4 region using the 515F (GTGYCAGCMGCCGCGGTAA)- 806R (GGACTACNVGGGTWTCTAAT) primer set was completed by the University of Exeter Sequencing Service using an Illumina MiSeq platform