RNA-seq of apple
The mRNAs used in NGS were from the same source as in DRS. The mRNAs were randomly interrupted by Covaris ultrasonic crusher, and then cDNA libraries were constructed using NEBNext UltraTM RNA Library Prep Kit for Illumina. After quality checks with Qubit 2.0 Fluorometer and Agilent 2100 bioanalyzer, the libraries were sequenced using an Illumina Novaseq 6000.