GSM8461674: Microglia, invitro, WT, 2h, rep3; Mus musculus; RNA-Seq
The RNA from microglia culture was isolated using Direct-zol RNA Miniprep Plus Kit following manufactures instruction. RNA quality and concentrations were accessed using Bioanalyzer RNA kit and samples with RIN value >8.0 were selected for further processing. RNA libraries for RNA-seq were prepared using NEBNext Low input RNA library Prep Kit for Illumina (NEB, E6420) following manufacturer's protocols.