Soil metatranscriptome of Sap site, replicate 1
Total RNA was extracted using commercial kits and checked with agarose gel, Qubit, Nanodrop, and Agilent 2100. Ribosomal RNA was removed with Ribo-Zero, and libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit. Sequencing was performed using Illumina HiSeq X platform to generate 160 bp paired-end reads.