CT26 shNT (CRX2096272)
Tumor xenografts were mechanically and enzymatically to obtain single-cell suspensions. Intratumoral CD45+ T cells were isolated by fluorescence-activated cell sorting and resuspended in PBS with 0.2% FBS. Cell concentration was adjusted according to the 10x Genomics Chromium 3' v3.5 protocol for single-cell library preparation. Libraries were sequenced on an Illumina NovaSeq 6000 platform with a depth of 90000 reads per cell