GSM8462265: KO IMQ 2; Mus musculus; RNA-Seq
RNA from isolated Langerhans cells was extracted using R2060 DirectZol RNA MicroPrep kit (Zymo Research). 10 to 70 ng of total RNA was used for construction of sequencing libraries. RNA libraries for RNA-seq were prepared using SMARTer Stranded Total RNA-Seq Kit v2- Pico Input Mammalian according to manufacturer's protocols.