GSM8830533: SKG-PBS mouse BMDC, (1>3)-beta-D-glucan stimulated, Mouse 32, biol rep4; Mus musculus; RNA-Seq
BMDCs stimulated in culture for 12 hours were harvested and stained with fluorophore-conjugated antibodies anti-CD11c APC (Biolegend, N418, #117310) and anti-CD11b PerCP-Cy5.5 (Thermo Fisher, M1/70, #45-0112-82), and with the viability dye DAPI (BioLegend) for dead cell exclusion. BMDCs were sorted on the FACSAria Fusion (BD Biosciences) into the Mag-MAX Lysis Buffer (Ambion, AM1830). RNA was isolated using the Mag-MAX total RNA kit (Ambion, AM1830) according to the manufacturer's protocol, and RNA yields and quality were assessed on Bioanalyzer (Agilent). rRNA depletion and library preparation were performed using the KAPA RNA HyperPrep Kit with RiboErase (Roche).