GSM8513004: TRANSATRA Patient 05-001 screening; Homo sapiens; RNA-Seq
enrichment of blasts: aMACS sort for CD34 and CD117 positive cells, (Miltenyi: CD34 MicroBead Kit, human, #130-046-702, CD117 MicroBead Kit, human, # 130-091-332)total RNA isolation: Machery&Nagel Kit, NucleoSpin RNA, #740955.250 Library prep: Illumina, TruSeq Stranded Total RNA Library Prep Gold, Read: length 2x75, Depth 100 M