GSM9573527: hTSC B31 Rotational culture; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNeasy Micro Kit (QIAGEN) with genomic DNA removal by ribonuclease-free deoxyribonuclease I. Libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) with NEBNext Poly(A) mRNA Magnetic Isolation Module for poly(A) selection. Libraries were sequenced on Illumina NovaSeq X Plus (150 bp paired-end reads, 3 Gb per sample).