RNA-Seq of homo sapiens: adult female colorectal cancer
Total RNA was isolated using RNeasy Micro Kit (Qiagen). Traces of DNA were removed with TURBO DNA-free Kit (ThermoFisher Scientific). Ribosomal RNA was depleted using NebNext rRNA Depletion Kit (Human/Mouse/Rat) (New England Biolabs). Transcriptomic library was consrtucted with NEBNext Ultra II Directional Library Prep Kit for Illumina (New England Biolabs) and Multiplex Oligos for Illumina (96 Indexes) (New England Biolabs). Paired-end library was sequenced on the Illumina HiSeq 2500 instrument with 2 125 cycles using HiSeq SBS Kit v4 (Illumina).