MeRIP-seq of dental pulp stem cells under hypoxia
This dataset was generated from human dental pulp stem cells (DPSCs) cultured under normoxic (21% O) and hypoxic (3% O) conditions. Total RNA was extracted using TRIzol reagent (Thermo Fisher Scientific) and quantified (NP80 Touch, IMPLEN). Poly(A) RNA was enriched from total RNA using Dynabeads Oligo (dT)25 magnetic beads (Thermo Fisher Scientific) and fragmented. For MeRIP-seq, fragmented RNA was subjected to immunoprecipitation using an anti-m6A antibody (Synaptic Systems, #202003). Sequencing libraries were constructed from both the input (mRNA-seq) and IP (MeRIP-seq) samples. The process included cDNA synthesis, adapter ligation, and PCR amplification. Libraries were quantified and size-selected, followed by paired-end sequencing (PE150) on an Illumina NovaSeq 6004 platform (LC-Bio Technology Co., Ltd., Hangzhou, China).