GSM9378491: rhAmpSeq, K562, CHD4 guide 12, ADSS1 singleplex; Homo sapiens; OTHER
Genomic DNA was extracted by column purification. RhAmpSeq libraries were prepared by the rhAmpSeq CRISPR Library Preparation protocol (IDT), using 50 ng of genomic DNA per sample. Pooled <i>ADSS1</i> libraries were combined with pooled multiplex libraries at a 1 to 4 ratio by mass.