GSM9609990: HaCat cells, pdna-3, Day3; Homo sapiens; RNA-Seq
Extract total RNA using the TRIzo reagent kit Following sample quality control, library construction was performed primarily through the following steps: (1) Eukaryotic mRNA was enriched using Oligo(dT)-coated magnetic beads.  (2) mRNA was randomly fragmented by adding Fragmentation Buffer.  (3) Using the fragmented mRNA as template, the first and second strands of cDNA were synthesized, followed by cDNA purification.  (4) The purified double-stranded cDNA underwent end repair, A-tailing, and adapter ligation. Fragment size selection was then performed using AMPure XP beads.  (5) Finally, the cDNA library was enriched via PCR amplification.