Meta-transcriptomic sequencing of oropharyngeal swabs: adult patient male (14-35 age range) sampled in Spring
Total RNA from each pool was extracted using the RNeasy Plus Universal Mini Kit (QIAGEN). RNA of every five samples were grouped into pools based on same month, same age range (14-35 or 36-65 age range) or same gender. Ribo-off rRNA Depletion Kit (Human/Mouse/Rat) (Vazyme) was subsequently used to remove host ribosomal RNA. Meta-transcriptome library preparation of each pool was carried out using the VAHTS Universal V8 RNA-seq Library Prep Kit for Illumina (Vazyme).