GSM9432955: F-A12; Mus musculus; OTHER
Eyes (n=3) were fixed in 4% paraformaldehyde (PFA) overnight. The superior nasal (SN) quadrant was dissected and paraffin-embedded. Sections (5µm) were mounted onto glass slides within a 35.3mm x 14.1mm area, dried at 58°C for 3 hrs. The tissues were subjected to the GeoMx DSP workflow (Bruker Spatial Biology, San Jose, CA). Prior to selecting ROIs, serial sections were stained with hematoxylin or double immunostained for K8 and K12 to identify tissue boundaries. ROIs included corneal, transitional (containing some limbus and cornea), limbal and bulbar conjunctival epithelia.