NanoSeq of Arabidopsis thaliana: UVC treated Col-0 shoot
DNA was extracted from flash frozen tissue and sonicated to 150bp. 200ng of DNA was size selected to 150bp and end blunted. Fragments were A-tailed and had nicks blocked using a dATP and ddBTP mix. Adapter stubs were ligated and library concentration was measured by qPCR. This concentration was used to determine the optimal portion of the sample to sequence for the targeted read number. This portion was PCR amplified and sequenced. For the untreated Col-0 libraries, 3 replicates of this portion were amplified and sequenced.