RNA-seq of Pelagomonas calceolata under iron stress
RNA extraction was performed using the TRIzol Reagent (Thermo Fisher Scientific) and DNA excluded with DNase I (TURBO DNA-free kit, Thermo Fisher Scientific). RNA was purified using Agencourt RNAClean XP beads (Beckman Coulter) and ribosomal RNA was depleted using a Ribo-Zero rRNA Removal Kit (Illumina). cDNA libraries were prepared with a ScriptSeq v2 RNA-seq kit (Illumina) and quality evaluated on an Agilent 2200 TapeStation instrument. Sequencing of 120 cDNA libraries was performed on the Illumina MiSeq platform.