GSM9433269: Bronchoalveolar lavage fluid from patients with intermediate-stage lung injury; Homo sapiens; RNA-Seq
Aortic leukocytes were lysed using TRIzol reagent (Thermo Fisher Scientific) following repeated pipetting to achieve a single-cell suspension without visible clumps, then snap-frozen in liquid nitrogen for RNA extraction. Single-cell RNA-seq libraries were constructed using the Chromium Single Cell 3' Library & Gel Bead Kit v2 (10× Genomics) following the manufacturer's protocol. Barcoded cDNAs were amplified, purified with the SPRIselect reagent (Beckman Coulter), and assessed for quality using the Agilent 5400 system.