GSM8633965: I16R020c04: frontalCortex_WT_5m_3; Rattus norvegicus; RNA-Seq
Total RNA, miRNA, and DNA were simultaneously extracted using the DNA/RNA/microRNA Universal Kit (Qiagen) using the manufacturer protocol Using the TruSeq RNA Sample Prep Kit (Illumina) and 100 ng of total RNA for each sequencing library, poly-A selected paired-end sequencing libraries (125 bp read length) were generated according to the manufacturer's instructions. Libraries were sequenced on an Illumina HiSeq 2500 platform at a depth of 20-35 mio reads each.