Amplicon sequencing of integrated lentivirus library barcodes: Moderna Donor 11, Post Dose 2, Dilution 1:640, Rep 1
Genomic DNA was isolated using the chemagic 360 instrument and chemagic DNA Tissue 10 Kit H96 (Revvity). Integrated barcodes were amplified via 30 cycles of PCR, and an additional 25 cycles of PCR were performed to attach index sequences. PCR 2 products were pooled by volume, and a double-sided (0.65x-0.85x) size selection was performed on the pool with SPRIselect (Beckman Coulter). The size-selected pool was submitted to the MIT BioMicro Center for sequencing on Singular's G4 Sequencer using their 150 PE kit. The SRA does not currently provide a controlled vocabulary entry for this instrument; Illumina's NovaSeq 6000 was selected as the most similar available platform.