GSM8995956: Meningioma Cells from Patient NU3971, Control Treatment; Homo sapiens; RNA-Seq
RNA was isolated using the All Prep RNA/DNA Mini kit (Qiagen; cat #80204), after tissue lysis and homogenization steps. TruSeq Stranded Total RNA Library Preparation Kit (Illumina) was used to prepare sequencing libraries from total RNA. This included Ribo-Zero rRNA Removal, cDNA synthesis, 3′ end adenylation, adapter ligation, library PCR amplification, and validation steps.