RNA-Seq of Homo sapiens: MDA-231 breast cancer cell line
Total RNA was extracted from CAL-51 and MDA-MB-231 breast cancer cells using RNeasy Mini Kit (Qiagen). mRNA was enriched using oligo-dT magnetic beads. Stranded RNA-seq libraries were constructed using the VAHTS Stranded mRNA-seq Library Prep Kit for Illumina v2 (Vazyme Biotech, cat. no NR612) following the manufacturer's protocol. Libraries were size-selected for an average insert size of 400 bp and sequenced on an Illumina NovaSeq 6000 platform with paired-end 150 bp reads.