WGS of Burkholderia pseudomallei from human clinical sample
Whole-genome metagenome sequencing libraries will be prepared using KAPA DNA HyperPrep kit. In brief, the DNA is sheared using Covaris ultrasonicator. Sheared DNA is subjected to a sequence of enzymatic steps for repairing the ends and tailing with dA-tail followed by ligation of indexed adapter sequences. These adapter ligated fragments are then cleaned up using SPRI beads. Next, the clean fragments are indexed using limited cycle PCR to enrich the adapter ligated molecules. Finally, the amplified products are purified and checked for quality and quantity before sequencing. Prepared libraries will be sequenced on Illumina NovaSeq X plus to generate 2x150bp reads/sample. The generated sequence data will be checked for necessary quality control.