GSM8996333: WT+Vehicle 1; Mus musculus; RNA-Seq
Freshly dissociated single-cell suspensions were loaded to 10X Genome GenCode Single-cell instrument to generate GEMs (Gel Bead-In-EMlusion). ScRNA-seq libraries were subsequently prepared following the manufacturer's protocol of Chromium Next GEM Single Cell 3' Regent Kits v3.1. Libraries were then sequenced using the Novaseq 6000 platform with paired-end sequencing (PE150) mode.