Environmental DNA sequences collected from soil, Estonia, Linnamae, arable field
DNA was extracted from a 5 g sample of dried soil using a PowerMax(R) Soil DNA Isolation Kit. AM fungal sequences were amplified from soil DNA extracts using AM fungal specific primers for the small-subunit (SSU) ribosomal RNA gene: WANDA and AML2. Sequencing was performed on the Illumina MiSeq platform, using a 2x300 bp paired-end sequencing approach. Importantly, DNA concentrations were not equalised between samples prior to sequencing.