16S rRNA amplicon sequencing of the snout and cloacal microbiota of Crocodylus intermedius
DNA extraction was performed as described in Rudolf et al., (2018). The 16S rRNA gene was amplified using universal V4 primers 515F (5-GTGCCAGCMGCCGCGGTAA-3) and 805R (5-GGACTACHVGGGTWTCTAAT-3) following protocols from the Earth Microbiome Project (https://earthmicrobiome.org/protocols-and-standards/). Sequencing was performed on an Illumina MiSeq platform.