WT_1 (CRX1312943)
Single-cell suspensions were loaded onto a 10X Genomics Chromium instrument to capture single cells according to the manufacturer's instructions for the 10X Genomics Chromium Single Cell 3' kit (V3). The following cDNA amplification and library construction steps were performed according to the standard protocol. Libraries were sequenced on an Illumina NovaSeq 6000 sequencing system at a minimum depth of 20,000 reads per cell.