GSM9535935: Amplicon long-read sequencing NanogSE_wt_rep2; Mus musculus; OTHER
For SMRT sequencing, 1 × 10^6 GFP-positive mESCs were isolated by FACS, and genomic DNA was extracted using the FastPure Cell/Tissue DNA Isolation Mini Kit (Vazyme, DC102-01) following the manufacturer's protocol. For long-amplicon PCR, 30 ng of genomic DNA was combined with 1 µL each of 10 µM forward and reverse primers, 12.5 µL of 2 × LongAmp enzyme mix (NEB, M0287S), and nuclease-free water to a final volume of 25 µL. PCR products were purified using DNA purification beads, and approximately 1 µg of amplicon was used for SMRTbell library preparation and subsequent SMRT sequencing.