GSM8722854: ESC, R70H, Replicate 2; Mus musculus; ATAC-seq
The ATAC-seq of the mESCs and mNPCs WT and RNF2 R70H was performed according to manufacturer's protocols (Active Motif 53150). Briefly, 1 × 10⁵ cells were resuspended in ATAC Lysis Buffer to extract nuclei. The nuclei were then resuspended in the Tagmentation Master Mix, and incubated for 30 minutes at 37°C in a Thermomixer (Eppendorf). Subsequently, the DNA was purified using the DNA purification columns provided in the kit. The library was prepared using the indexing reagents and Q5 polymerase provided in the ATAC-seq kit, following the manufacturer's protocol (Active Motif, 53150).