GSM9078372: Human derived non-small cell lung cancer cells, circRNA, control group, biol repl2; Homo sapiens; ncRNA-Seq
Total RNA was extracted from the tissue using TRIzol® Reagent according the manufacturer's instructions. Then RNA quality was determined by 5300 Bioanalyser (Agilent) and quantified using the ND-2000 (NanoDrop Technologies). Only high-quality RNA sample was used to construct sequencing library. smallRNA libraries were generated using QIAseq miRNA Library Kit (Qiagen) following manufacturer's recommendations, and mRNA+lncNRA+circRNA libraries were generated by Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus.