GSM9104339: Post-GIM cellsmultiplexed MU; Homo sapiens; RNA-Seq
1 × 10^6 cells at the desired stage were detached with TrypLE or Accutase and resuspended in ice-cold PBS for multiplexing. Cell viability was above 90% for all samples as determined by Trypan Blue exclusion. Cells from each donor at each stage along the induction process were labelled with a unique set of Cell Multiplexing Oligos using Single Cell Gene Expression v3.1 (Dual Index) kit (10X Genomics, CD000391). After labelling and washing, an equal number of cells from each patient at the same stage in the induction process were mixed for encapsulation, Gel Bead-in Emulsion (GEM) generation and library preparation according to manufacturer's protocol (Chromium controller, 10X Genomics).