GSM8823662: scRNAseq of tagged library: 12hr after ligand, replicate 1, batch1; Homo sapiens; RNA-Seq
Samples were processed with the Chromium Next GEM Single Cell 3' HT Kit (10x Genomics, CG000416 Rev C). Pooled tag libraries treated with HyT36 were loaded across 3 GEM wells at 88 k cells each per timepoint with the goal of recovering 39 k singlets per well after filtering. Vehicle and untagged controls were loaded each into a single well at 30.5 k and 14.5 k cells, respectively, with the goal of recovering 17 k and 8 k singlets after filtering Chromium Next GEM Single Cell 3' HT Kit