GSM8605553: ChIPbis_AtT_ERPax7_Tam48h_rep2; Mus musculus; ChIP-Seq
Nuclei lysates were sonicated and protein-DNA complexes were isolated with specific antibodies. Fragments of 200-500 bp were kept and six to twelve independent ChIPs were pooled. Bisulfite conversion was done with EZ DNA Methylation-Lightning (Zymo Research). Libraries and flow cells were prepared by the IRCM Molecular Biology Core Facility according to Illumina's recommendation