Insight into the regulatory mechanisms of growth under water deficit: a case study in perennial ryegrass
Insight into the regulatory mechanisms of growth under water deficit: a case study in perennial ryegrass
Right after the last watering (at 9 a.m.), crown tissue samples were collected from three replicates of each genotype (control). The next six sampling points were performed on the 3rd, 4th, and 5th day of mild drought stress, in the morning (9 a.m.) and afternoon (mid-light cycle, at 4 p.m.). Lastly, plants tissue was sampled 2 hrs after rewatering. Plnats were grown in commercially availiable substrate for 5 week old plants (25-30 tillers) in greenhouse. They were then transferred to growth chambers (16/8 hrs light/dark at 25 C) an kept for 4 days with normal watering to acclimate to new environment. RNA extraction was carried out using Qiagen RNeasy Plant Mini kit The libraries were prepared according to the TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA, USA)