GSM8862936: ChIP-seq, HIF1α, Hypoxia, MiaPaCa-MYB KO-HIF1αOE, replicate 1; Homo sapiens; ChIP-Seq
MYB and HIF1α ChIP were carried out using ChIP-IT Express Enzymatic Kit (Active Motif, CA, USA) as per manufacturers instruction manual. ChIP-seq libraries were prepared using NEB Next Ultra II DNA library prep kit. Briefly, 10-15 ng of DNA was used as starting material for input and IP samples. DNA libraries were sequenced using illumina NextSeq 500 plateform.