16S rRNA amplicon sequencing of colonic microbes C1.DLY.33 (CRX846189)
Microbial DNA  from each pig colon fecal samples was extracted with the fecal genomic DNA extraction (DP712, Norcross, Georgia, USA), then V3-V4(338F-806R) of 16S rRNA was amplified by two-step PCR.Finally, the amplicons were sequencedon an Illumina MiSeq platform (Illumina, San Diego, USA)  in paired-end sequenced (2 x 306 bp) .