GSM8633801: Day_6_8_10_rep1; Homo sapiens; RNA-Seq
Samples (9704-BC, 11288-BC) for single-cell RNA sequencing were harvested with the 10x Flex fixation kit based on the manufacturer's manual. Samples (4830-KJ) were collected using accutase and resuspended in ice cold PBS. Cells were barcoded using the 10x Genomics Chromium system (part numbers 1000268, 1000120, 1000215). For quality control cDNA was quantified by Qubit High Sensitivity DNA assay and Agilent TapeStation For all samples, the University of Michigan Advanced Genomics Core performed library preparation.