GSM8942455: BMP4_d2_E; Homo sapiens; ATAC-seq
Cells were lysed in ATAC-seq Lysis Buffer and nuclear pellet was resuspended in Transposition Mix and incubated at 37ºC as described in Grandi et al. 2022. Transposition reaction was terminated by adding 5x (vol/vol) DNA Binding Buffer. DNA clean up was perfomed with DNA Clean & Concentrator-5 kit (Zymo Research). Transposed fragments were barcoded with unique combination of adapter sequences. NEBNext Ultra II Q5 (NEB) was used for library amplification for 9 cycles of PCR. Libraries were cleaned using DNA Clean & Concentrator-5 kit (Zymo Research), and size selection was performed with AMPure XP beads (0.55x sample volume followed by 1.8x sample volume).