RNA-Seq of  homo sapiens: LS174T cells
mRNA was extracted using TRIzol reagents (Thermo Fisher Scientific, Waltham, MA, USA, catalog no. 15596026) according the standard protocol. Library preparation and sequencing were performed on DNBSEQ-T7 system by BGI Genomics Ltd (BGI). Briefly, mRNA was enriched using poly(T) oligo-attached magnetic beads and reverse-transcribed to double stranded cDNA, which was then subjected to adaptor ligation. The products were amplified by PCR and library quality was analyzed by Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA).