GSM8463455: WT - replicate 3; Mus musculus; RNA-Seq
RNA was harvested using Rneasy mini plus kit (Qiagen) Libraries were prepared from 500 ng of tal RUNA using SureSelect XT HS2 mRNA Library prepration System (Agilent) according to manufacture protocol (Version A1, September 2020). It utilize using Poly-A Selection Module (Cat.No 5190-6411), cDNA Module (Cat.No 5500-0149) and XT HS2 Index Primer Pairs (Cat.No 5191-5688). Adaptor-ligated cDNA libraries were amplified using 9 PCR cycles.