RNA-seq of Acinetobacter sp. DL27
RNA-seq libraries were constructed using a strand-specific, rRNA-depletion method. Briefly, after rRNA was removed via targeted ssDNA probe hybridization and digestion, the enriched RNA was fragmented for first-strand cDNA synthesis. Second-strand cDNA was then synthesized with the incorporation of dUTP to preserve strand directionality. Following simultaneous end-repair, A-tailing, and adapter ligation, the dUTP-containing second strand was selectively degraded by UDG enzyme. Finally, the remaining strand-specific fragments were PCR-amplified using P5/P7 primers."