16S amplicon sequencing of veterinarians NBC18
DNA was extracted from samples using the DNEasy PowerSoil Pro Kit (Qiagen) according to the manufacturer's protocol. Barcoded PCR primers annealing to the V1-V2 region of the 16S rRNA gene (Fwd, 5-AGAGTTTGATCCTGGCTCAG-3; Rev, 5-TGCTGCCTCCCGTAGGAGT-3) were used for library generation. The resulting library was sequenced on the Illumina MiSeq using 2x250 bp chemistry.