GSM9378741: LXC2; Sus scrofa; RNA-Seq
Total RNA was extracted from ileum and colon samples using the Trizol method. Poly(A) + mRNA was isolated from total RNA using Dynabeads Oligo(dT)25 RNA was reverse transcribed into cDNA using random oligonucleotides, Super Script II, DNA Polymerase I, and RNase H. Adapters were ligated to both ends of cDNA fragments. Double-stranded cDNA of approximately 450 bp were selected using the AMPure XP bead-based purification system (Beckman Coulter, Beverly, CA, USA). Adapter-ligated libraries were enriched through polymerase chain reaction (PCR) amplification using the Illumina PCR Primer Cocktail for 15 cycles. The amplified libraries were purified using the AMPure XP system and quantified using the Agilent High Sensitivity DNA Assay on a Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). Paired-end sequencing was performed on the NovaSeq X platform (Illumina, San Diego, CA, USA).