GSM9262083: JCC300 Gene Expression reaction 1; Homo sapiens; RNA-Seq
Sorted B cells and Tfh cells were encapsulated in gel beads in emulsion (GEMs) using a 10x Genomics Chromium Controller. Reverse transcription and cDNA amplification according to the 10x Genomics protocol CG000186 Chromium Single Cell VDJ Feature Barcoding technology Rev D. Gene expression and Feature Barcode libraries were prepared according to 10x Genomics protocol 10x Genomics protocol CG000186 Chromium Single Cell VDJ Feature Barcoding technology Rev D.