GSM8459779: R817; Homo sapiens; RNA-Seq
The PBMC samples were retrieved from the liquid nitrogen tank and thawed for scRNAseq library generation Briefly, the viable PBMC were loaded into chromium microfluidic chips and barcoded with a 10x Chromium Controller (10x Genomics). RNA from the barcoded cells was subjected to sequencing library generation with reagents from a Chromium Single Cell 3′ v.2 Reagent Kit (10x Genomics) according to the manufacturer's protocol. The library was sequenced on Illumina sequencer (NovaSeq 6000) at a 150 bp paired-end  sequence length and 400 million read throughput per library.