high-throughput transcriptome sequencing of THP-1 cells
The high-throughput transcriptome sequencing of THP-1 cells was performed using the Illumina HiSeq sequencing platform. Total RNA was extracted from the samples using Trizol. The mRNA libraries were constructed from qualified total RNA samples, and the recovered RNA was quantified using the Qubit 2.0 RNA quantification kit. The samples were mixed equally in a 1:1 ratio and subjected to high-throughput sequencing. Thereafter, low-quality reads were filtered out using Trimmomatic. The quality-controlled sequencing sequences were aligned with the reference genome using HISAT2, and the alignment results were analyzed using RseQC. The expression levels were estimated using StringTie.