GSM9610098: NK cells from CHB patient 3; Homo sapiens; RNA-Seq
Total RNA was extracted from FACS-sorted peripheral blood NK cells using MJzol Animal RNA Isolation Kit according to the manufacturer's instructions, followed by purification with RNAClean XP Kit and RNase-Free DNase Set. RNA integrity was assessed using Agilent 2100 Bioanalyzer or Agilent 4200 TapeStation, and RNA quantity and purity were measured using Qubit 2.0 and NanoDrop ND-2000. mRNA was enriched from purified total RNA using VAHTS mRNA Capture Beads (Human/Mouse/Rat). RNA-seq libraries were prepared using VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina. Library concentration was measured using Qubit 2.0, and fragment size distribution was assessed using Agilent 4200 TapeStation. Libraries were sequenced on the Illumina NovaSeq 6000 platform with 150 bp paired-end reads.