GSM9056021: Bone marrow-derived macrophages, Legionella pneumophila and long-chain polyphosphate treated, biological replicate 3; Mus musculus; RNA-Seq
Total RNA was isolated using the RNeasy Mini Kit (Qiagen). 100 ng was used for the construction of sequencing libraries. Novogene performed quality control on all RNA samples. RNA libraries were constructed and sequenced using the standard mRNA library preparation (poly A enrichment) protocol with paired-end sequencing (150 bp) by Novogene Corporation Inc. (Sacramento CA). This included messenger RNA purification from total RNA using poly-T oligo-attached magnetic beads, followed by fragmentation, cDNA synthesis, end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was quality checked using Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries were pooled and sequenced on Illumina NovaSeq 6000.