RNA-seq of Arabidopsis root sieve element markers at 24h of iron deficiency
RNA-seq of Arabidopsis root sieve element markers at 24h of iron deficiency
FACS-sorted protocols were collected from 5d old roots that had been iron deficiency-starved for 24h Growth on MS for 4d then transferred to iron deficient media for 24h Plants were transferred to iron deficiency media for 24h before sorting Sorted cells were immediately frozen on liquid nitrogen then RNA was extracted using the Qiagen RNeasy micro kit Extracted RNA was converted to cDNA using the  Takara SMARTer cDNA synthesis kit