GSM8996812: Multiome, Colon, TCDD, ATAC, Illumina NovaSeq 6000; Mus musculus; ATAC-seq
CD45+ immune cells and dendritic cells were isolated from the colon lamina propria and from the colon-draining mesenteric lymph nodes of C. rodentium infected mice. Single nuclei suspensions were prepared isolated according to the Demonstrated Protocol: Nuclei Isolation for Single Cell Multiome ATAC + Gene Expression Sequencing (CG00053 – Rev C, 10x Genomics), using the Low Cell Input Nuclei isolation protocol. Isolated single nuclei were transposed, then loaded on Chromium Chip and partitioned in nanolitre scale droplets using the Chromium Controller and Chromium Next GEM Single Cell Reagents (Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Kits User Guide, User Guide, CG000338)