EB_16 (CRX2210475)
Total microbial genomic DNA was extracted from soil samples using the E.Z.N.A. Soil DNA Kit (Omega Bio-tek, USA) following the manufacturer's instructions. DNA quality and concentration were assessed using agarose gel electrophoresis and a NanoDrop 2000 spectrophotometer (Thermo Scientific, USA). The 16S rRNA gene V3-V4 region was amplified with primer pair 338F (5'-ACTCCTACGGGAGGCAGCAG-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3'). PCR products were purified using the PCR Clean-Up Kit (YuHua, China) and quantified with Qubit 4.0 (Thermo Fisher Scientific, USA) prior to sequencing on the Illumina NextSeq 2000 platform.