scRNAseq of tumor
5'GEX libraries built from 20-50ng cDNA with 14 cycles PCR. 10X recomends 50 million clusters/sample when determining seq coverage. Sequencing read is as follows: Read 1 - 26 cycles, i7 index - 10 cycles, i5 index - 10 cycles, Read 2 - 90 cycles.