GSM8460910: H3K9me3_IP_iCKO; Mus musculus; ChIP-Seq
For ChIP-seq, the ChIP-DNA was processed into Illumina sequencing libraries with the TIANSeq Fast DNA Library Kit (NG102, TIANGEN) and enrichment with a slight modification involving size selection on an agarose gel. Total RNA was extracted using a RNAsimple Total RNA Kit (DP419, TIANGEN)；genomic DNA was extracted with nuclei dilution buffer (20 mM Tris Cl pH 8.0, 2 mM EDTA, 150 mM NaCl, 1% NP40, 1% SDS, and 1×PI).