GSM8605315: SE initiating cell, H3K27ac, ChIP, Rep1; Homo sapiens; ChIP-Seq
Cells grown on 10 cm culture dishes were subjected to crosslinking with 1% formaldehyde at room temperature for a duration of 10 minutes, followed by quenching with 0.125M glycine. Subsequently, the cells were lysed in ice-cold sonication buffer and sonicated using a Covaris M220 focused-ultrasonicator. The ChIP-seq libraries were prepared by following the manufacturer's instructions with the KAPA Hyper Prep Kit (Kapa Biosystems, KK8502), and were then sequenced on the NovaSeq 6000 platform (Illumina) utilizing 150 bp paired-end reads.