GSM9500770: Control_2; Mus musculus; RNA-Seq
Peripheral blood was collected from mice 6 hours after cecal ligation and puncture. CD45-positive immune cells were isolated using magnetic bead-based separation. Single-cell RNA-seq libraries were prepared using the Chromium Single Cell 3' Reagent Kits v3 (10x Genomics) according to the manufacturer's protocol. Briefly, isolated CD45-positive immune cells were loaded onto the Chromium Controller to generate single-cell Gel Bead-In-Emulsions (GEMs). Within each GEM, cells were lysed, and mRNA molecules were reverse transcribed to generate barcoded cDNA.