GSM8893218: Uterine immune cells, E13.5, Control_2; Mus musculus; RNA-Seq
Female mice (8 – 12 weeks old) were mated overnight with Balb/cJ wild-type male mice. The presence of a vaginal plug was designated as embryonic day (E) 0.5. At E13.5, pregnant mice were euthanized by cervical dislocation, and the myometrium was isolated from the pregnant uterus. Tissues were enzymatically digested into cell suspensions. Cells were enriched using Lympholyte-M (Cedarlane) and CD45-positive, IL-18R-positive cells were sorted using a FACS Aria II (BD) for sample preparation. Total RNA extraction was all performed using the SMART-Seq HT PLUS Kit (Takara Bio USA, CA, USA) following to the manufacturer's instructions. cDNA synthesis and library construction were all performed using the SMART-Seq HT PLUS Kit following to the manufacturer's instructions.