ChIP-seq of Spirodela polyrhiza clone 9509
Chromatin immunoprecipitation (ChIP) was conducted on Arabidopsis 10-day-old seedlings (WT Col-0) and Spirodela polyrhiza #9509 fronds. IP was performed using specific antibodies. anti-H3 (rabbit polyclonal, Abcam, #ab1791), anti-H3K27me3 (rabbit polyclonal, Millipore, #07449), anti-H3K4me3 (rabbit polyclonal, Abcam, #ab8580), anti-H3K9me1 (rabbit polyclonal, Abcam, #ab8896), or anti-H3K9me2 (mouse monoclonal, Abcam, #ab1220). Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, #E7103) according to the manufacturer's instructions.