Nanopore sequencing of rVSV-Rs3367-CoV_replicate 2
Viral RNA was isolated from rescue population supernatants. cDNA was then generated through reverse transcription with a VSV genome-specific primer. cDNA was amplified by PCR with VSV-specific primers flanking the spike gene. Following this, long-read DNA sequencing on the Oxford Nanopore Technologies (ONT) platform (Plasmidsaurus) was performed to identify mutations present in the viral population.