sequencing of Pasteurella multocida
Genomic DNA from Pasteurella multocida was extracted from overnight cultures using the Qiagen Genomic DNA Kit. DNA quality was assessed using a NanoDrop 2000 spectrophotometer (A260/280 ratio of 1.8) and agarose gel electrophoresis. Library preparation was carried out using the Illumina Nextera XT DNA Library Preparation Kit, with DNA fragmentation to an average size of 350 bp and adapter ligation. The libraries were sequenced on an Illumina NovaSeq 6000 platform in paired-end mode with 150 bp reads to achieve a coverage of 100. Raw sequencing data were processed using FastQC and Trimmomatic to remove low-quality reads and adapters. The cleaned reads were assembled with SPAdes, and the assembly was evaluated using QUAST. Genome annotation was performed using Prokka, and functional annotations were assigned via sequence homology searches against the NCBI NR database.