GSM8512930: CD8 Donor 1 SA control 20h; Homo sapiens; RNA-Seq
Cell pellets were stored at 4ºC with 50 μl of RNAlater (Invitrogen) until RNA purified using the RNAqueous-4PCR DNA-free RNA isolation kit following the provided protocol.  0.1-0.5 µg of total RNA pers sample was used after removal of ribosomal RNA using the Next rRNA depletion kit (New England Biolabs). RNA samples passed quality control and proceeded to library preparation using TruSeq Stranded mRNA library prep kit (Illumina) with IDT for Illumina - TruSeq RNA UD indexes (Illumina, 20022371) following manufacturer's protocol. Libraries were amplified using 12 cycles of PCR RNA seq libraries were amplified using 12 cycles of PCR, pooled, and sequenced on Illumina NovaSeq 6000 with 150x150 bp paired-end reads