RADseq:CsPS1
Genomic DNA was extracted using the CTAB protocol, digested with SbfI restriction enzyme, and ligated to barcoded Illumina P1 adaptors. After shearing, size selection, and pooling into three libraries, DNA was further processed by sonication, blunt-end repair, and adaptor ligation. The libraries were amplified and sequenced on an Illumina HiSeq 2500