Hi-C Illumina HiSeq X Ten sequencing of Bassozetus sp.2
Cells were crosslinked with 1% formaldehyde. The DNA was digested with MboI, and the resulting fragments were biotinylated and proximity-ligated to form chimeric circles. After ligation, crosslinks were reversed, and DNA was purified. The biotinylated DNA was sheared to 300-500 bp, and the biotin-labeled fragments were pulled down using streptavidin beads. Sequencing libraries were prepared using the Illumina TruSeq DNA Library Prep Kit and sequenced on the Illumina HiSeq X Ten platform to generate 2 x 150 bp paired-end reads.