GSM8794225: control,Mus musculus,biol rep 2; Mus musculus; RNA-Seq
Total RNA was isolated from the specified biological materials using TRIzol Reagent (Thermo Scientific, cat. #15596026) following the manufacturer's recommended protocol. 1 ug of total RNA was used for the construction of sequencing libraries. For library preparation, the RNA of each sample was used for stranded dual RNA sequencing library preparation using Ribo-off rRNA Depletion Kit (Vazyme, China, cat. #N406 for animal, cat. #N407 for bacteria) to deplete rRNA, and then components of KCTM Digital Stranded mRNA Library Prep Kit (Seqhealth Tech. Co., Ltd. Wuhan, China) following the manufacturer's instruction. The kit eliminates duplication bias and errors in PCR and sequencing steps, by using unique molecular identifier (UID) of 12 random bases to label the pre-amplified cDNA molecules.PCR products corresponding to fragments ranging from 200 to 500 base pairs were enriched,quantified and sequenced on a DNBSEQ-T7 platform using the PE150 mode to generate paired-end reads.