GSM8996007: Notch2_flox_CD11c_Cre_R1_GEX; Mus musculus; RNA-Seq
Sort purified CD11c+ cells derived from C57BL/6 or Notch2fl/fl CD11cCre+ spleens, sLNs and mLNs were multiplexed individually by labeling each sort purified cell suspension with a unique Hashtag (HTO) antibody. 10K sort purified CD11c+ cells from the spleen, sLN and mLN of either C57BL/6 or Notch2fl/fl CD11cCre+ mice were mixed and loaded on a 10x Genomics Chromium Single Cell Controller. Single cell capture, cDNA and library preparations were performed with the Single Cell 3' v3 Reagent Kit (10x Genomics) according to manufacturer's instructions. GEX sequencing libraries were loaded on an Illumina NovaSeq with high output cycle kits and sequenced paired-end using the following read length: Read1-28 cycles / Read2-8 cycles / Read3-91 cycles. HTO libraries were prepared according to manufacturer instruction and loaded on an Illumina NextSeq 500 with high output cycle kits and sequenced paired-end using the following read length: Read1 28 cycles / Read2 8 cycles / Read3 56 cycles for HTO libraries. The first 15 bases of R3 represented HTO barcode sequence.