GSM8722510: In vitro, IR adipocyte exosome treated, biological replicate 3; Mus musculus; RNA-Seq
Total RNA was extracted from 4T1 cells treated with IS and IR adipocyte derived exosome using the RNeasy Plus Mini Kit (QIAGEN, Cat. No. 74136), following the manufacturer's protocol. Exosomal RNA was extracted from culture media using the exoRNA Easy Kit(Qiagen, Cat. No. 77144).  RNA integrity was assessed using an Agilent 2100 Bioanalyzer. Libraries were constructed using the Illumina TruSeq Stranded mRNA Library Prep Kit, as per the manufacturer's protocol. Final libraries were checked for quality using an Agilent Bioanalyzer and quantified using a Qubit fluorometer.