GSM9537740: MCF10CA1a, ATAC-seq, biological replicate 1; Homo sapiens; ATAC-seq
Cells were treated with DNAse at a concentration of 200 U/mL and incubated at 37°C for 30m, then washed three times with PBS. Nuclei were then isolated using RSB-Lysis buffer incubation for 10m on ice. Cells were scraped from plates and entrifuged at 500xG for 5m at 4°C. Supernatant was removed, cell nuclei were washed in RSB-Wash buffer, and cells were spun again as before. Libraries were prepared according to the omniATAC protocol (CITE). Nuceli are first incubated with Tn5 transposase, then DNA is purified using Zymo DNA Clean and Concentrator kit. Transposed DNA is then amplified to add Illumina adapters using NEBNext High-Fidelity PCR kit. Libraries are assessed for fragment size using TapeStation and quantified on a fluorometer.