RNA-seq of Homo sapiens: Human umbilical vein endothelial cells + NICD1, biorep 3
Total RNA in the amount of 500 ng for each sample was used to prepare RNA-sequencing libraries using the "CORALL Total RNA seq Library Prep Kit" with poly-A RNA selection in accordance with the manufacturer's recommendations. The quality of the obtained libraries was verified by capillary electrophoresis on Agilent Bioanalyzer 2100 (Agilent Technologies). Finally, the libraries were sequenced on the Illumina NextSeq550 platform using one-sided reagents. All samples were sequenced simultaneously.