0h_1 (CRX2077214)
The RNA libraries were constructed using the TruSeq Stranded Total RNA Library Prep Kit. During second-strand cDNA synthesis, dUTP was used in place of dTTP, resulting in second-strand cDNA composed of A, U, C, and G bases. Prior to PCR amplification, the second strand was selectively degraded using uracil-N-glycosylase (UNG), ensuring that only the first-strand cDNA was retained in the final library.