AD4 (CRX2204899)
Sample testing: Precisely quantify the concentration of the sample and conduct integrity testing. Samples that pass the test are used for library construction. PCR system construction: Prepare the PCR reaction system by taking 30ng of DNA sample and the fusion primers. PCR amplification: Set the PCR reaction parameters and perform PCR amplification. Product purification: Purify using magnetic beads and dissolve in Elution Buffer, label it, and the library construction is completed. Library quality testing: Test the fragment range and concentration of the library. Sequencing on the instrument: When the library is qualified, perform sequencing on the instrument.