GSM9433343: Multiplexed, spleen, B6, naive CD4+ T cells, GEX; Mus musculus; OTHER
Mouse cells were first pre-enriched by negative selection, using the EasySep™ Mouse naive CD4+ T Cell Isolation Kit. Cells were sorted as singlet, viability dye-, CD4+, CD8-, CD44lo, CD62L+, CD25-, CXCR3-. Droplet-based 5′ end scRNA-seq was performed using the 10x Genomics platform and libraries were prepared and sequenced on a NextSeq2000 using 28×90bp reads for GEX libraries using the Chromium Single Cell NextGEM 5' v2 chemistry Reagent kit.