GSM8893209: Methylobacterium extorquens SLI 505 in 10 mM vanilliic acid, biological replicate B; Methylorubrum extorquens; RNA-Seq
40 mL of culture were harvested at mid-exponential phase (OD600 of 0.5-1 for all conditions) by centrifugation at 4°C at 2000 x g for 10 minutes. Total RNA was extracted using the Qiagen RNeasy Kit and quantified. rRNA depletion using the Ribo-Zero RNA Plus rRNA Depletion Kit (Illumina) was performed by SeqCoast Genomics. Library preparation and Illumina Hi-Seq sequencing was performed by SeqCoast Genomics.