GSM8463277: OB_pat_LuC_43; Homo sapiens; RNA-Seq
To isolate RNA, 10 µM - thick paraffin slices were trimmed from each FFPE tissue block using a microtome. RNA was extracted from FFPE slices using QIAGEN RNeasy FFPE Kit following the manufacturer's protocol. RNA 6000 Nano or Qubit RNA Assay kits were used to measure RNA concentration. RNA was processed and  subjected to isolation of generation of sequencing libraries according to Suntsova et al., 2019 [PMID: 31015567].