GSM8943448: PH1, periodontitis, hypertension; Rattus norvegicus; miRNA-Seq
The gingival tissues of the mandibular first molars were collected for RNA extraction. Sequencing libraries were generated using NEB Next® Multiplex Small RNA Library Prep Set for Illumina® （NEB  E7300L）. Briefly, 3' and 5' adaptors were ligated to 3' and 5' end of small RNA, respectively. Then the first strand cDNA was synthesized after hybridazition with reverse transcription primer. The double-stranded cDNA library was generated through PCR enrichment. After purification and size selection, libraries with insertions between18~40 bp were ready for sequencing on Illumina sequencing with SE50. Subsequently, library quality was assessed on the Agilent 5400 system（Agilent，USA）and quantified by QPCR（1.5nM）. The Qualified libraries were pooled and sequenced on Illumina platforms with SE50 strategy in Novogene Bioinformatics Technology Co., Ltd (Beijing, China), according to effective library concentration and data amount required.