ddradseq data of Arizona elegans
We collected ddRADseq data using the Peterson et al. 2012 protocol modified to use separate indexing reads. We digested genomic DNA with SbfI and MspI (New England Biolabs) in a single reaction and used Solid Phase Reversible Immobilization (SPRI) beads (Beckman Coulter) to purify the digestions prior to ligating uniquely bar-coded adapters with T4 ligase (New England Biolabs). Libraries were size-selected between 400 and 5300 base pairs (bp) using a Pippin Prep size fractionator (Sage Science). The final library amplification used proofreading Taq and Illumina's indexed primers. The fragment size distribution and concentration of each pool was determined on an Agilent Bioanalyzer and qPCR was performed to determine library concentrations.