GSM9105730: Aortic valves, control, pooled 4 valves, IgG; Mus musculus; OTHER
Aortic valves were dissected from 12- week old mice and snap frozen in liquid nitrogen and stored in -800C. Nuclei were isolated and followed Active Motif's protocol (catalog #53160) with minor modification. The nuclei were bound with concanavalin A beads and incubated w with antibodies targeting phospho-HDAC3, HDAC3, H3K27ac, or IgG control. Secondary antobodies were incubated for an hour followed by pA-Tn5 transposomes for another hour. The DNA was tagmented and extracted as per the protocol. Indexed libraries were prepared following Active Motif's protocol (catalog #53160) and sequenced on an Illumina MiSeq platform with 25-bp paired-end reads.