Transcriptome sequencing of Microcystis aeruginosa
RNA-seq strand-specific libraries were prepared following the TruSeq RNA sample preparation Kit from Illumina (San Diego, CA, USA), using 5 g of total RNA. Briefly, rRNA removal was performed using the RiboZero rRNA removal kit (Epicenter, WI, USA), and the RNA was then fragmented using fragmentation buffer. Subsequently, cDNA synthesis, end repair, A-base addition, and ligation of the Illumina-indexed adaptors were carried out according to Illuminas protocol. The libraries were size selected for cDNA target fragments of 200  300 bp on 2% Low Range Ultra Agarose, followed by PCR amplification using Phusion DNA polymerase (NEB) for 15 PCR cycles. After quantification using TBS380, the paired-end libraries were sequenced using the Illumina NovaSeq 6000 sequencing platform (150bp*2, Shanghai BIOZERON Co., Ltd).