GSM9303797: A_D_Fc_7_2; Hordeum vulgare; RNA-Seq
Spike material was ground in liquid nitrogen, and extraction of total RNA was performed with 100 mg of each sample using the GeneMATRIX Universal RNA Purification Kit (EURx Molecular Biology Products), following the manufacturer's instructions. Libraries for 3'-RNA-sequencing (Moll et al., 2014) were prepared using the QuantSeq 3'mRNA-Seq Library Prep Kit (Lexogen, Austria) according to the manufacturer's instructions. Library sizes were quantified with a Qubit Fluorometer 2.0 and a Qubit DNA High Sensitivity Assay Kit. Ready-to-load pools of cDNA libraries were determined with qPCR. An Agilent 2010 Electrophoresis Bioanalyzer with the High Sensitivity DNA Assay was used to check the integrity and mRNA fragment size distribution of each library.