AMEP412-6-1 (CRX1803710)
Using fragmented mRNA as the template and random oligonucleotides as primers, the first strand of cDNA was synthesized in the M-MuLV reverse transcriptase system. Subsequently, the RNA strand was degraded with RNaseH, and the second strand of cDNA was synthesized using dNTPs as the raw material in the DNA polymerase I system. The purified double-stranded cDNA was subjected to end repair, an A tail was added and the sequencing connector was connected. cDNA of about 250-300bp was screened with AMPure XP beads for PCR amplification and the PCR product was purified again using AMPure XP beads. Finally, the library was obtained.