GSM8830341: IgG; Homo sapiens; RIP-Seq
Cell lysate was immuno-precipitated by using antibody to protein of hnRNPA2B1 or IgG along with protein A/G magnetic beads. After proteinase K digestion, the RNA co-immunoprecipitated with proteins was purified by phenol/chloroform extraction and precipitated in ethanol. Then, RNA was re-suspended in RNase-free water and subjected to GenSeq rRNA Removal Kit (GenSeq, Inc.) to remove rRNA. RNA libraries were constructed by using rRNA-depleted RNAs with GenSeq Low Input RNA Library Prep Kit (GenSeq, Inc.), according to the manufacturer's instructions. Libraries were controlled for quality and quantified using the BioAnalyzer 2100 system (Agilent Technologies, Inc.). Library sequencing was sequenced with 150bp paired end reads.