GSM8942285: organoid_Ctrl; Homo sapiens; RNA-Seq
At 72 hours and 2 weeks following the final radiation dose, organoids were collected and dissociated into single-cell suspensions using the Papain Dissociation Kit (#LK003150, VWR) according to the manufacturer's instructions. Cell counts were determined using a cell counter, and the resulting suspensions were processed for sequencing using the PIPseq T2 kit. Sequencing libraries were prepared according to PIPseq T2 instructions, with 12 cycles for cDNA amplification and 8 cycles for library amplification, depending on the DNA amount. The libraries were quantified using a Qubit 4 Fluorometer and a TapeStation 4200 and sequenced as per manufacturer recommendation on a NovaSeq X Plus – 25B 2 x150bp.