Amplicon sequence of invertebrate metagenome: macroinvertebrates
All PCR products including negative controls were pooled for sequencing. PCR amplicon libraries were prepared using the Novaseq 6000 SP Reagent Kit v1.5 Library Preparation Kit, and 2  250 bp paired-end sequencing was executed on an Illumina Novoseq 6000 System (Novogene Co., Ltd., Tianjin, China) with PCR-free library protocol.