QuantSeq of EGR1 KO or WT immortalised bone marrow derived macrophages during Salmonella Typhimurium infection
QuantSeq of EGR1 KO or WT immortalised bone marrow derived macrophages during Salmonella Typhimurium infection
A clonal population of immortalised bone marrow derrived macrophages following CRISPR KO of Egr1 or CRISPR against no target (i.e. WT) were infected with Salmonella enterica sv. Typhimurium (strain SL1344) at a multiplicity of infection of 10 or mock-infected. Gentamicin was added to cultures at 15 min post infection to kill extracellular bacteria. RNA was extracted from cultured cells at the indicated time point by Trizol purification according to manufacturer's instructions Libraries were generated with the Lexogen QuantSeq 3' mRNA-Seq Library Prep Kit FWD according to manufacturer's instructions