MEPP_FK3 (CRX1918453)
Total RNA was extracted from tissues using TRIzol Reagent according to the manufacturer's instructions. RNA purification, reverse transcription, library construction, and sequencing were performed by Shanghai Majorbio Bio-Pharm Biotechnology Co., Ltd. (Shanghai, China). RNA-seq libraries were prepared with the Illumina Stranded mRNA Prep, Ligation kit (Illumina, San Diego, CA) using 1 ug total RNA per sample. Library concentration was measured with a Qubit 4.0 fluorometer. Paired-end sequencing (2 x 150 bp) was carried out on an Illumina NovaSeq 6000 instrument.