RNA-Seq of Mus musculus: 26-month-old female brain
Total RNA was isolated from the brain tissues of 26-month-old C57BL/6J mice (treated with 0.5 g/kg phosphoenolpyruvate (PEP) or vehicle control via intraperitoneal injection for 6 months) using the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturers instructions. RNA integrity was assessed using an Agilent 2100 Bioanalyzer.