vRNA seq of Orthoebolavirus zairense grown in microgla cultures P10 MOI=1
EBOV sequence analysis was performed using culture supernatants via reverse transcription with SuperScriptIV Reverse Transcriptase (Thermo Fisher Scientific; #18090010) and subsequent amplification with random primers. A stranded, paired-end library was prepped using the TruSeq RNA exome (Illumina, San Diego, CA, USA; #RS-301-2001) before sequencing on a MiniSeq (Illumina, San Diego, CA, USA; # SY-420-1001) using a MiniSeq High Output Kit 300 cycles (Illumina, San Diego, CA, USA; #FC-420-1003).