GSM8943849: CUT&Tag of IgG in mouse VSMCs (vasostatin-2); Mus musculus; OTHER
CUT&Tag was performed following the standard protocol using the NR1D1 antibody. Briefly, permeabilized nuclei were incubated with primary antibody followed by secondary antibody and pA-Tn5 adapter complex. DNA fragments released after tagmentation were extracted using a DNA purification kit. DNA fragments were amplified using indexed primers and a limited-cycle PCR. Libraries were purified using AMPure XP beads and quality-checked using Bioanalyzer. Sequencing was performed on Illumina NovaSeq 6000 with paired-end 150 bp reads.