GSM8862505: Dissociated organoids, KOLF2.1J, batch 2; Homo sapiens; RNA-Seq
The culture medium was aspirated and the cells were lifted with accutase and washed with HBSS. The cells were resuspended in TRIzol and RNA was precepitated from the aqueous phase following phase sepration of TRIzol with chloroform. Purified RNA samples were sent for sequencing to Genewiz Inc (Azenta Life Sciences). Illumina sequencing libraries with PolyA selection were prepared in-house by Azenta.