GSM8893261: ESC_ChIP-H3K27me3_WT_rep1; Mus musculus; ChIP-Seq
Native ChIP was performed as described (Goldberg et al. 2010). Briefly, ESCs were harvested and treated with Mnase, resulting in a population consisting mainly of mono- to trinucleosomes. Chromatin was isolated and used for ChIP according to standard procedures. ChIP-seq. Libraries were constructed according to the Illumina Nextera protocol.