RNA-Seq of Cylinder canonicus: adult specimens from Mayotte
One Cylinder canonicus specimen was dissected on ice to extract the venom gland in form of a long and thin duct. The venom duct was placed in 1 mL of TRIzol regent (Invitrogen, Carlsbad, CA, USA) in an Eppendorf tube and the purified total ribonucleic acid (RNA) was extracted by following the manufacturers instructions [9]. Next, mRNA was purified from the retrieved total RNA using the Oligotex mRNA Mini Kit (Qiagen, Valencia, CA, USA). Finally, mRNA extracts were submitted to Montpellier GenomiX (MGX, BioCampus Montpellier, CNRS) for sequencing. Complementary DNA (cDNA) libraries were constructed and sequenced using a high-throughput Illumina sequencer (Illumina Inc., San Diego, CA, USA) with a TruSeq Stranded mRNA Sample Prep Kit and a TruSeq Library indices following the manufacturers instructions. Paired-end sequencing (2x 150 bp) yielded several millions of short-read sequences after filtering poor quality reads.