DIVA U2OS SFPQ 2 20 pMol  +4OHT
DIvA U2OS cells were transfected with 20 pmol siRNA targeting SFPQ or a non-targeting control (see RNA interference section). DNA breaks were induced with 4-OHT or left uninduced. Total RNA was extracted from ~1  10 cells using the Direct-zol RNA Miniprep Kit (Zymo Research, Cat#R2050) following the manufacturers protocol, and eluted RNA was stored at 80C. Samples were submitted to Novogene for human mRNA sequencing, including poly(A) enrichment and library preparation, and sequenced on a NovaSeq X Plus platform (PE150) to a depth of 6 G raw reads per sample.