GSM9893897: HTO library for patient 1934AL (ex-vivo, MS5); Homo sapiens; RNA-Seq
Cells were washed once using cell staining buffer (BioLegend, Cat. Nr. 420201) and barcoded with 1:100 of one type of Total-Seq C antibody in cell staining buffer. Suspensions were incubated for 30 minutes at 4°C. Samples were washed once with cell staining buffer and once with PBS + 0.4% BSA before counting and pooling at equal fractions. The cells were counted and viability checked using a Luna FX7 cell counter and AO/PI staining. The single-cell workflow (including feature barcoding and TCR libraries) was conducted following the protocol “Chromium GEM-X Single Cells 5' v3 Gene Expression with Feature Barcoding technology for Cell Surface Protein User Guide” (10X Genomics, protocol Nr. CG000734).