GSM8634329: Ischemia - Vena porta infusion 3; Sus scrofa; RNA-Seq
Total RNA was isolated using the miRNEAsy kit (Qiagen) according to the manufacturer's protocol and eluted in RNAse-free water. Samples were mRNA enriched using oligodT Library construction was performed at the Novogene sequencing facilities, Cambridge, UK.  In short, purified mRNA was fragmented into small pieces with fragment buffer at appropriate temperature. Then First-strand cDNA was generated using random hexamer-primed reverse transcription, followed by a second-strand cDNA synthesis. afterwards, A-Tailing Mix and RNA Index Adapters were added by incubating to end repair. The cDNA fragments obtained from previous step were amplified by PCR. The single strand circle DNA (ssCir DNA) was formatted as the final library.