GSM9309365: Su140 NC14, HiC, rep5; Drosophila melanogaster; Hi-C
For each genotype, six biological replicates of 100 hand-staged NC14 embryos were analyzed. Libraries were prepared directly on beads using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7645S and #E6440 or equivalents), with adapter ligation performed on-bead. Prior to PCR amplification (12–13 cycles), ligated DNA fragments were released by heating.