RNA-Seq of zebrafish ovary treated with artificial light at night (ALAN) against a normal light dark sample
RNA-Seq of zebrafish ovary treated with artificial light at night (ALAN) against a normal light dark sample
The adult female zebrafish (identical in age, body weight, and gonadal status) were randomly divided into four experimental groups in different photic conditions: Normal: LD = 12H L:12H D, Treated: (i) continuous light for one week, LLW (7 days), (ii) for one month, LLM (30 days), and (iii) for one year, LLY (365 days). All the samplings were performed at same time (12.00 H) The ovary was removed by dissecting the body cavity respectively. Sample for whole transcriptome sequencing was stored in RNAlater (Life Technology, USA)], and at -80ì§¸C. Total RNA was isolated from the sample using Trizol (Invitrogen) method for RNA extraction. SENSE mRNA-Seq Library Prep Kit V2 (Lexogen, Austria) was used as per the protocol to generate the libraries.