Whole exome sequencing of LGG349 cell line
Samples were subjected to quality control according to sample type and products requirements. Genomic DNA was randomly fragmented. The fragmented genomic DNA was selected to a certain average size. The fragments were subjected to end-repair and then was 3 adenylated. And then adaptors were ligated to the ends of these 3 adenylated fragments. The PCR reaction system and program are configured and set up to amplify the product, and PCR products were purified. Take a certain amount of PCR products for hybridization. Use corresponding hybridization and wash kits for hybridization and wash steps. The PCR reaction system and program are configured and set up to amplify the product. The corresponding library quality control protocol will be selected depending upon product requirements. Single-stranded PCR products are produced via denaturation. The reaction system and program for circularization are subsequently configured and set up. Single-stranded cyclized products are produced, while uncyclized linear DNA molecules are digested. Single-stranded circle DNA molecules are replicated via rolling cycle amplification, and a DNA nanoball (DNB) which contain multiple copies of DNA is generated. Sufficient quality DNBs are then loaded into patterned nanoarrays using high-intensity DNA nanochip technique and sequenced through combinatorial Probe-Anchor Synthesis (cPAS).