Hi-C
The purified nuclei were digested with 100 units of DpnII and marked by incubating with biotin-14-dCTP. Biotin-14-dCTP from non-ligated DNA ends was removed owing to the exonuclease activity of T4 DNA polymerase. The ligated DNA was sheared into 300600 bp fragments, and then was blunt-end repaired and A-tailed, followed by purification through biotin-streptavidin-mediated pull down.