GSM8794475: Control, replicate 4, RRBS; Homo sapiens; Bisulfite-Seq
Genomic DNA was isolated using Quick-DNA Microprep kit (Zymo Research, Irvine, CA, Cat#: D3021) following the manufacturer's instructions. Starting input genomic DNA (~100 ng) was digested with 30 units of MspI (New England Biolabs, Ipswich, MA, Cat#: R0106S). Fragments were ligated to pre-annealed adapters containing 5'-methyl-cytosine instead of cytosine according to Illumina's guidelines (Illumina Inc., San Diego, CA, USA). Adaptor-ligated fragments ≥50 bp in size were recovered using the DNA Clean & Concentrator-5 (Zymo Research, Cat#: D4003). The fragments were then bisulfite-treated using the EZ DNA Methylation-Lightning Kit (Zymo Research, Cat#: D5030).