whole genome resequencing of Ganoderma sinense :Ganoderma sinense ZG-2
After the sample genome DNA is tested and found to be qualified, it is fragmented by mechanical means (ultrasound) and then purified, end-repaired, 3'-end A-added, ligated to sequencing adapters, and subjected to agarose gel electrophoresis for fragment size selection. The fragmented DNA is then amplified by PCR to form a sequencing library. The library is first quality-checked before being used on the Illumina Novaseq 6000 platform for sequencing.