DNBSEQ Sequencing of Irpex lacteus: isolate BJFC24 from human cervical swab
Genomic DNA was fragmented, selected, and prepared with ends repair, A-tailing at the 3' end, and adapter ligation.  Following PCR amplification and quality control, the PCR products were denatured into single strands, and circularization was performed to obtain single-stranded circular DNA.  Un-circularized DNA was digested, and single-stranded circular DNA molecules were amplified by rolling circle amplification (RCA) to form DNA nanoballs (DNB) containing multiple copies.  These DNBs were added to high-density chips for joint probe-anchored synthesis (cPAS) sequencing.