GSM9262381: FD AML, CCS1477, 3; Mus musculus; RNA-Seq
At the end of the treatment protocol cells were lysed with Trizol. RNA was extracted according to Meyer et al., 2009. RNA quality and quantity were measured by Agilant 2200 Tape Station. Then samples with a RIN>8.0 were processed by ribosome depletion, stranded True-seq, paired-end 150 base pair (bp), 30 million reads. RNA Seq libraries were generated with Illumina TruSeq Stranded mRNA HT kits.