DZ0065 (CRX2147760)
The bacterial 16S rRNA V3 region was amplified using universal primer pair, and the PCR reactions were conducted using Phusion High-Fidelity PCR Master Mix. The PCR products were next determined by 2% agarose gel, and DNA ranging from 400 to 450 bp was collected for further use. The libraries were constructed and then sequenced on Ion Torrent S85 platform.