RNA-Seq of mus musculus: adult male heart
mRNA was enriched from total RNA using Oligo(dT)-coated magnetic beads. The enriched mRNA was fragmented, then reverse transcribed into first-strand cDNA using random hexamers, followed by second-strand cDNA synthesis. The double-stranded cDNA was purified, end-repaired, A-tailed, and ligated with adapters. After size selection using AMPure XP beads, the library was amplified by PCR