Smart-Seq of male blastocysts from mothers bearing the OgtT931A mutation.
Smart-Seq of male blastocysts from mothers bearing the OgtT931A mutation.
Embryos were generated through IVF, then cultured in a standard mammalian cell incubator in KSOM medium. Separation of RNA from single embryos with SPRI beads. Modified Smart-Seq2 protocol using SuperScript IV RT and tagmentation procedure previously described (Hennig et al. 2018).