GSM9262124: Atrx+_CTCF; Mus musculus; OTHER
500,000 cells were washed with 1X Wash Buffer and incubated with Concanavalin A Beads for 30 minutes. Cells were incubated overnight at 4°C using 1ug of anti-CTCF antibody (Diagenode, Cat # C15410206) or IgG (Diagenode, Cat # C15410206) with orbital mixing. The following day, the cells were washed using 1X Wash Buffer containing Protease Inhibitory Cocktail and Digitonin and incubated with Guinea Pig Anti-Rabbit secondary antibody, followed by Tn5 transposomes and Tagmentation procedures. DNA was eluted using column filters and libraries constructed using i7 and i5 indexing primers, following PCR conditions. Eluted libraries were assessed for quality using a Bioanalyzer and subjected to Nextseq500 MID-300 using 150 bp pair-end read format at the MDACC-ATGC facility.