GSM8461777: h.CD34+, shControl - Hydro C, Day 5; Homo sapiens; RNA-Seq
Total RNA was isolated from 106 cells using Trizol and reverse transcribed with RNase OUT kits. Quantitative real-time PCR was performed. To assess BCL11A knockdown, RNA was extracted with the Qiagen RNeasy Micro Kit and reverse transcribed with the iScript Reverse Transcription Supermix. mRNA levels and expression ratios were calculated. RNAseq of BCL11A-microdeletion patients was performed. The day 3 and day 5 progeny of CD34+ cells were profiled with the Illumina libraries constructed using Prime-seq and sequenced at read lengths 28x8x94.