Dnmt1 off targets of treated hepatocytes from WT mice
Genomic DNA from mouse tissues were isolated by direct lysis. Locus-specific primers were used to generate targeted amplicons for deep sequencing. First, input genomic DNA was amplified in a 10-l reaction for 26 cycles using NEBNext High-Fidelity 2x PCR Master Mix (NEB). PCR products were purified using Sera-Mag magnetic beads (Cytiva) and subsequently amplified for six cycles using primers with sequencing adapters. Approximately equal amounts of PCR products from each sample were pooled, gel purified, and quantified using a Qubit 3.0 fluorometer and the dsDNA HS Assay Kit (Thermo Fisher Scientific). Paired-end sequencing of purified libraries was performed on an Illumina MiSeq.