GSM8459699: PBS Control and Dual ADC Treatment mixed sample; Mus musculus; RNA-Seq
A 10X single-cell library was prepared using the Chromium Fixed RNA Kit (Cat# 1000497, 10X Genomics). Approximately 16,500 cells were hybridized with probe hybridization to target polyadenylated RNA. The hybridized cells were then loaded onto the Chromium X instrument (PN-1000326, 10X Genomics) to generate barcode-labeled single-cell gel beads-in-emulsion (GEMs) with a target cell count of ~10,000. The GEMs were subsequently lysed using a recovery agent to release the hybridized RNA. cDNA was synthesized from the released RNA, followed by amplification and sample index PCR for indexing. The quality control of cDNA was evaluated using an Agilent Bioanalyzer to ensure the final library molecules contained P5 and P7 priming sites for Illumina sequencers. Single-cell library construction was initiated using the 10X barcoded ligated probe products, and sequencing was performed using the NovaSeq 6000 flow cell 100-cycle kit (Illumina, San Diego, CA, USA) in a Read1:i7:i5 format of 28:10:10:90 bp.