GSM8720303: U118 cells, WT, Day2; Homo sapiens; RNA-Seq
RNA was extracted using chloroform. 1ug of total RNA was used for the construction of  sequencing libraries. According to the characteristics of the mRNA containing polyA tail structure, the mRNA in total RNA was first enriched with olig dT, and the purified mRNA was randomly interrupted with divalent cations. The segmented RNA was used as template and random oligonucleotides as primer to synthesize double-stranded cDNA. The purified double-stranded cDNA was added by end repair /dA tail and connected by sequencing splices. The linked products were purified and fragment size sorted to obtain the cDNA fragments of the required size. Then PCR amplification was performed and the PCR products were purified again, and finally a library was sequenced.