GSM8896214: 105C, 72 hour monolayer, replicate 2; Homo sapiens; RNA-Seq
To obtain RNA from cell lines grown as monolayer or in suspension the 105C and KOC-7c cell lines were grown for 3 days on either tissue culture plastic or ULA plates. On day 3, RNA was extracted and purified using RNEasy Kit (Qiagen, cat. 74106). Concentration and purity were measured using NanoDrop One Microvolume UV-Vis spectrophotometer (Thermo Scientific). VAHTS Total RNA-Seq (H/M/R) Library Prep Kit for Illumina (PN NR603) from Vazyme Biotech Co. Ltd. was used to create indexed libraries which were sequenced using the Illumina NextSeq platform (150-bp; single-end reads).