Complete Genome Sequence of the Fowlpox Virus Vaccine Strain (Buddett strain) of the Livestock Research Institute of Bangladesh
Metagenomic libraries were constructed utilizing the iNextEra library preparation protocol. The DNA was tagmented using Illumina bead-linked transposomes (Illumina DNA library prep, Illumina, Inc., San Diego, CA, USA) and 2X TMP buffer (Ref) in a total volume of 6 L, comprising 3 L of 2X TMP buffer, 0.5 L of bead-linked transposome, and 2.5 L of DNA, followed by incubation at 53C for 30 minutes. After tagmentation, a limited-cycle PCR step finalized the Illumina-compatible adapters with dual-indexes utilizing custom iNextEra primers for sequencing of pooled libraries