GSM9536841: f_standard_saline_oLN_389; Rattus norvegicus; RNA-Seq
RNA was extracted from bone using PureLink RNA Mini Kit (#12183018A, Invitrogen, Carlsbad, CA, USA) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dTTP for non strand specific library or dUTP for strand specific library. For the strand specific library, it was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification