Destruction Complex Base editing Screen_CBE
Genomic DNA was extracted from either unsorted or 7-TCF-mScarletsorted eHAP1 cells using a standard DNA extraction kit. Sequencing libraries were prepared using the NEBNext High-Fidelity 2X Master Mix, amplified by PCR, and quantified with Qubit. Single-end 75 bp sequencing was performed on an Illumina NextSeq 500/550 instrument. Libraries represent independent biological replicates from a CRISPR base editing screen in eHAP1 cells.