GSM8829396: Murine CAFs treated with 4 mM glutamine + DMSO + 25 pg/mL IL1a, Rep2; Mus musculus; RNA-Seq
Total RNA was extracted using a PureLink RNA Mini Kit (Invitrogen). PolyA RNA isolation and library preparation was performed with the Watchmaker mRNA Library Prep Kit (Watchmaker Genomics: 7BK0001) with xGEN Stubby adaptors (IDT, Cat# 336338420) and xGEN 10nt UDIs (IDT, Cat# 336338436). Libraries were sequenced (2x75bp) with the Element Biosciences AVITI Sequencing platform using the AVITI 2x75 High Output Cloudbreak Freestyle Kit (Element Biosciences, Cat# 860-00015).