GSM8721211: 555_dTAG_H4K20me2_T0_rep3; Mus musculus; OTHER
After the appropriate treatment, 1% formaldehyde for 10 min. Then, glycine was added to quench the reaction and cells were subsequently lysed. Sonication was performed on isolated nuclei using a Covaris E220. DNA fragments were immunoprecipitated, purified fragments were biotinylated, libraries were prepared, EdU-biotin fragments were pulled-down and sequenced. Libraries were costructed using the KAPA Hyperprep kit and Illumina-compatible indexed adapters (Pentabase) following manufacturer's instruction. For the amplification step, 11 PCR cycles were used. Subsequently, the DNA underwent size selection using Agencourt AMPure XP beads (Beckman Coulter, A63881) to obtain fragments between 200-700 bp. Libraries were validated using Agilent Fragment Analyzer.