TrAEL-seq, 0.5mM, Hydroxyurea treated, Replicate 1
For TrAEL-seq, 1  106 asynchronous cells were harvested post-treatment in absence or presence of HU. Cells were washed in 5 ml L buffer (10 mM Tris HCl (pH 7.5), 100 mM EDTA, 20 mM NaCl), embedded in CleanCut agarose and digested overnight at 50 in 500 l digestion buffer (10 mM Tris HCl (pH 7.5), 100 mM EDTA, 20 mM NaCl, 1% sodium N-lauroyl sarcosine, 0.1 mg/ml Proteinase K) before washing with TE and PMSF. TrAEL-seq was performed using the updated multiplexing protocol as described