GSM8828910: hepAGT -/- liver, 14d WD, #26; Mus musculus; RNA-Seq
After euthanasia, liver samples were snap-frozen in a homogenization solution (#Z305H, Promega) containing 1-thioglycerol (#A208B, Promega). Subsequently, mRNA was extracted using Maxwell RSC simplyRNA Tissue Kits (#AS1340, Promega) according to the manufacturer's protocol. RNA samples were shipped to Novogene (CA) for bulk mRNA sequencing. The sequencing library was generated from total mRNA (1 µg) using NEBNext UltraTM RNA Library Prep Kits for Illumina (New England BioLabs, MA). cDNA libraries were then sequenced by a Next Generation NovaSeq platform, (HWI-ST1276, Illumina), in a pair-end fashion to reach more than 1,500,000 reads.