Malus pinkspire
After passing the library check, the different libraries were sequenced in Illumina after pooling them according  to the effective concentration and the target sequencing output data volume, yielding 150bp paired-end reads. The  basic principle of sequencing is to synthesize and sequence at the same time. Four types of fluorescently labeled  dNTPs, DNA polymerase, and junction primers were added to the sequencing flow cell for amplification. When  extending the complementary strand of each sequencing cluster, each fluorescently labeled dNTP added emits  corresponding fluorescence, and the sequencer captures the fluorescence signals, and converts the light signals  into sequencing peaks through computer software, so as to obtain the sequence information of the fragment to be  tested.