GSM8794521: RNA_8C_Nr5a2_KD_rep3; Mus musculus; RNA-Seq
The zona pellucida was removed by treatment with acidic Tyrode. 10 x 8-cell embryos were washed five times in M2 medium and then quickly washed in PBS. Embryos were lysed in lysis buffer containing RNase inhibitor and ERCC spike-in on ice for 15min and then snap frozen with liquid nitrogen. cDNA was prepared using a SMART-seq v4 Ultra-Low-Input RNA kit for Sequencing (Takara, 634888). Library was prepared by a Nextera XT DNA Library Prep Kit (Illumina, FC-131-1024) according to the manufacturer's protocol. Purified DNA libraries were sequenced on a Novaseq 6000 (Illumina), or AVITI (Element Biosciences) with paired-end reads.