Isoseq of Ajwa Date Palm Root
Total RNA libraries were prepared using 300 ng RNA for synthesis of cDNA using BioLabs NEBNext kit following the manufacture protocol. The sequencing libraries were prepared using the Pacbio SMRT BELL PREP KIT 3.0 followed by binding using either PACBIO Sequel II Binding Kit 3.1 or REVIO POLYMERASE KIT using the primers given by the manufacturer.