metagenomic sequencing of rat fecal bd15 (CRX2103048)
After extracting the total DNA from the samples, primers were designed based on the conserved regions. Sequencing adapters were added to the ends of the primers, followed by PCR amplification. The PCR products were then purified, quantified, and normalized to construct a sequencing library. The constructed library was first subjected to quality inspection, and qualified libraries were sequenced using the Illumina NovaSeq 6000 platform. The raw image data files generated by high-throughput sequencing (e.g., Illumina NovaSeq and other sequencing platforms) were converted into raw sequencing sequences (Sequenced Reads) through Base Calling analysis. The results were stored in the FASTQ (abbreviated as fq) file format, which contains the sequence information of the sequencing reads and their corresponding sequencing quality information.