Repetat 2 for D7 in Chondrogenesis with PEMFs (CRX844989)
Total RNA was extracted from the pellet cultured after specific timepoint. After total RNA was extracted, eukaryotic mRNA was enriched by Oligo(dT) beads.Then the enriched mRNA was fragmented into short fragments and reversly transcribed into cDNA. The purified double-stranded cDNAfragments were end repaired, A base added, and ligated to Illumina sequencing adapters. The resulting cDNA library was sequenced using Illumina Novaseq6004.