Mus musculus feces 16S rRNA
This study aims to investigate the transcriptomic landscape and molecular mechanisms underlying traumatic painful neuroma formation following peripheral nerve injury. Using a murine sciatic nerve transection (SNT) model, total RNA was extracted from injured sciatic nerve stumps and associated dorsal root ganglia (DRG) at 4 weeks post-surgery, with sham-operated mice serving as controls. The experimental design includes three biological replicates per group to ensure statistical robustness. Stranded mRNA-seq libraries were constructed and sequenced on an Illumina NovaSeq 6000 platform, generating 150 bp paired-end reads with approximately 40 million reads per sample. The primary objective is to identify differentially expressed genes and key signaling pathwaysparticularly those involving the NGF/TrkA/RhoA axisthat drive fibrotic structural remodeling and nociceptive sensitization in the injury microenvironment. This dataset provides a foundation for dissecting the hierarchical relationship between etiological drivers of structural pathology and symptomatic pain transmission, and supports the development of mechanism-based therapeutic strategies for neuropathic pain.