YPQ231202mixISO-seq-Oct-23
High-integrity total RNA (RIN >= 7) is reverse-transcribed into full-length cDNA, cleaned, amplified, end-repaired, A-tailed, and ligated with SMRTbell adapters (optionally multiplexed), followed by magnetic bead clean-up, QC, and direct sequencing without assembly to obtain full-length isoform sequences.