GSM9262192: miR-4485-3p|+1 mimics transfection replicate1; Homo sapiens; RNA-Seq
Total RNA was extracted using the TRIzol reagent (Invitrogen Cat# 15596018CN) from Calu-3 cells with miR-4485-3p|+1 mimics or control mimics transfection Total RNA subjected to polyA+ RNA enrichment with TruSeq RNA Library Preparation Kit v2 (Illumina) and prepared into the cDNA library according to the Illumina RNA-Seq instruction.