GSM8997628: Combo_rep3; Homo sapiens; RNA-Seq
Samples were trypsinized (0.05% trypsin-EDTA), pelleted, and subjected to RNA extraction using RNeasy Lipid Tissue Mini Kit (samples were stored at -80˚C prior to RNA extraction). RNA-seq library preparation was performed using Poly(A) selection, where polyadenylated mRNA transcripts were selectively enriched and the sequencing was performed in the Illuminas NextSeq 2000 platform with 30 million read pairs per sample