G1_2-ATAC-Seq (CRX1743139; CRA026198)
The ATAC-seq experiment was performed with slight modifications based on published protocols. Fresh Bryophyllum pinnatum tissues were immediately homogenized after harvest and placed into pre-cooled D-Sorbitol buffer. The homogenate was filtered twice through a 30 um cell strainer. After staining the generated crude cell nuclei with 4',6-diamidino-2-phenylindole (DAPI), 50,000 cell nuclei were sorted using a flow cytometer (BD FacsAria II SORP) in 400 uL pre-cooled D-Sorbitol buffer. The sorted cell nuclei were collected by centrifugation and immediately mixed with TD buffer and 2 uL TTE mixture (Vazyme, TD501). After incubation at 37 degrees C for 30 minutes, the reaction was terminated, and DNA was purified using the MinElute PCR Purification Kit (Qiagen, 28006). ATAC-seq libraries were prepared using NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541L) and TruePrep Index Kit V2 for Illumina (Vazyme, TD202). Library fragments were size-selected using VAHTS DNA Cleans Beads (Vazyme, N411) and sequenced on an Illumina NovaSeq 6000 system (paired-end 151 bp).