GSM9541930: Normal Chow Diet Fed; Mus musculus; OTHER
For high-fat diet studies, fresh frozen mouse livers were embedded in Neg-50 and stored at -80 °C until sectioning. After sectioned at 10 μm thickness, sections were adhered to capture areas by brief warming onto Visium v1 slides (10x Genomics). For humanized liver studies, livers were fixed in 4% paraformaldehyde (PFA) for 3 hours, cryoprotected in PFA + 30 % sucrose for overnight, embedded in OCT, and tissue blocks were kept at -80 °C until sectioning. After sectioned at 10 μm thickness, Visium v2 (prepared with CytAssist) was performed using the CytAssist instrument. For both studies, the sample preparation was performed according to the manufacturer's guidelines. Briefly, tissue sections were stained with hematoxylin and eosin (H&E), imaged using a brightfield microscope for histological reference and spatial registration. RNA capture, reverse transcription, second-strand synthesis, cDNA amplification, and library construction were performed. Libraries were indexed, quality-controlled, and sequenced on an Illumina platform using paired-end sequencing.