GSM9263387: mda-mb-231_IDB-003_10mpk_rep7; Homo sapiens; RNA-Seq
Tumor samples were first flash frozen with liquid nitrogen and pulverized to ensure thorough disruption. Total RNA and protein were extracted using the AllPrep RNA/Protein kit (Qiagen), following the manufacturer's protocol. RNA fractions were subsequently subjected to additional purification and DNase treatment with the RNA Clean and Concentrator kit (Zymo Research) to remove residual genomic DNA and improve RNA integrity. Strand-specific cDNA libraries were generated using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) according to the manufacturer's instructions. Total RNA was depleted of rRNA with the NEBNext rRNA Depletion Kit v2 (New England Biolabs). Libraries were PCR-amplified with NEBNext Multiplex Oligos for Illumina and size-selected with AMPure XP beads (Beckman Coulter).