GSM9573980: CHOP_KO_Con_H3K27ac_SET3_rep2; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 min at room temperature and quenched using 0.125 M glycine for 5 min, followed by washing with cold PBS. After cell lysis, chromatin was sheared by sonication using a Qsonica sonicator(30 s ON/30 s OFF, 10 cycles, repeated three times). Lysates were pre-cleared by incubation with protein A/G magnetic beads (Invitrogen) prior to immunoprecipitation. Pre-cleared chromatin was incubated overnight at 4 ℃ with the indicated antibodies. Tagmentation was then performed directly on bead-bound chromatin using the Nextera DNA Library Prep kit (Illumina) followed by two washes to remove excess transposase. Bead-bound chromatin was incubated overnight  for reverse cross-linking, and the recovered DNA was PCR-amplified using Nextera PCR primers. Amplified libraries were size-selected and purified using AMPure XP beads (0.65x–0.9x)