GSM9759110: Control_Col_WT_1; Arabidopsis thaliana; RNA-Seq
For VIM overexpression and mutant lines, total RNA was extracted from leaves of 4 to 12-week-old T1 plants with the Monarch Total RNA Miniprep Kit (N2010, NEB) and tested with Qubit RNA BR Assay (Q33223, Thermo Fisher). For Ler x Cvi RILs, total RNA was extracted from bulk seedlings following the TRIzol extraction method. For VIM overexpression and mutant lines, library preparation and 150 bp paired-end sequencing were performed by Novogene on an Illumina NovaSeq X Plus platform. For Ler x Cvi RILs, libraries were constructed using the TruSeq Stranded mRNA Library Kit (No.20020594, Illumina) with a custom dual index workflow. Paired-end 150 bp sequencing was performed on an Illumina NovaSeq 6000 platform.