GSM9789198: Liver tumor, Slc1a5flox/flox, replicate 1; Mus musculus; RNA-Seq
Tumor tissues were mechanically and enzymatically dissociated into single-cell suspensions. Dead cells and debris were removed, and live cells were sorted or enriched as indicated for each sample. RNA capture was performed from single cells during single-cell 3′ RNA-seq library preparation. Single-cell 3′ RNA-seq libraries were constructed using the ABcellar Pro Single Cell 3′ RNA-seq Lib Prep Kit (ABclonal, RK20380) according to the manufacturer's protocol.