GSM9263440: WT MA10-infected mouse 2; Day 6; Mus musculus; RNA-Seq
A snip from the lower inferior lobe was taken and RNA was extracted from murine lung tissue preserved in trizol by bead beating (TissueLyserII, Qiagen), followed by a phenol-chloroform extraction and RNA Isolation Kit (Qiagen). RNA quality was assessed using Agilent Tape Station RNA kit. cDNA libraries were prepared using the NEB ultra-directional library preparation kit 2.0 following manufacturer's protocol