GSM8896949: Alveolar macrophage, no stimulation,after vaccine, Rep1; Homo sapiens; RNA-Seq
Total RNA is extracted using RNeasy Plus Kit from Qiagen which includes gDNA eliator columns, following the manufacturer's protocol. RNA QC was performed using the Agilent 4200 TapeStation RNA ScreenTape. mRNA was enriched using the NEBNext Poly(A) mRNA Magnetic Isolation Module with 200 ng of total RNA input. Sequencing libraries were generated using the NEBNext Ultra II Directional RNA Library Prep kit and Multiplex Oligos for Illumina (Unique Dual Index Primer Pairs Set 3). Library QC was performed using the Agilent 4200 TapeStation D1000 ScreenTape to assess fragment sizes and using qPCR to determine the functional library concentration. Libraries were normalized, pooled, and QC checks were repeated on the final library pool prior to sequencing.