ddRADseq of Castilleja coccinea
Genomic DNA was extracted using a Promega Maxwell 16 (Madison, WI, USA) at University of Illinois at Chicago, Research Resources Center, DNA Services Facility. The restriction enzymes EcoRI and MspI were used to generate double-digested fragments (ddRADseq) for sequencing following the protocol of Peterson et al. (2012) at the Global Biologics (Columbia, MO, USA). The completed libraries were sequenced with 150 base pairs length on an Illumina HiSeq 2500 platform.