WES of bladder cancer case 1
Peripheral blood (310 mL) is collected in EDTA or stabilizing tubes, genomic DNA is isolated from the buffy coat with silica or magnetic-bead kits, and yield/quality are assessed (e.g., Qubit, TapeStation). DNA is fragmented to ~150200 bp by enzymatic or mechanical shearing, followed by end-repair/A-tailing, ligation of unique dual-indexed adapters, cleanup, and limited PCR amplification. Exonic regions are enriched by hybrid capture with a whole-exome biotinylated probe set, captured on streptavidin beads, stringently washed, and subjected to post-capture PCR. Final libraries are QCd for size and concentration, pooled equimolarly, and sequenced on a Illumina Hiseq