GSM8635222: High Avidity TCR; Mus musculus; RNA-Seq
Sorted low, medium, and high avidity PDPRMUT specific CD8 TIL from MethA bearing mice were subject to 5' 10x genomic sequencing, feature bar code sequencing, and VDJ sequencing. Three libraries were generated for each condition using the Library Construction Kit, 5' Feature Barcode Kit, and Single Cell Mouse TCR Amplification Kit (10X Genomics) using the Chromium Next GEM Single Cell 5' Reagent Kit v2 (Dual Index) CG000330 Rev D protocol before Next Generation Sequencing with NovaSeq (Illumina Inc.).