GSM9468351: Thymus epithelial cells, NOD.AireWT/WT, 4 to 8 weeks, biol rep 3; Mus musculus; RNA-Seq
Mouse thymus was surgically harvested and digested into single-cell suspensions. CD45-expressing cells were depleted using magnetic beads. Cells were sorted for EpCAM expression using a Sony MA900 cell sorter. RNA was isolated using the Qiagen RNeasy micro kit. cDNA libraries were prepared using the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1).