ITS from rhizosphere soil of Chimonobambusa neopurpurea
Total DNA from soil samples was extracted using the E.Z.N.A. Soil DNA Kit. DNA quality and purity were assessed by agarose gel electrophoresis and NanoDrop spectrophotometry. The extracted DNA was used as a template to amplify the V3-V4 region of the bacterial 16S rRNA gene and the ITS1 region of fungal ITS using primer pairs 338F/806R and ITS1F/ITS2R, respectively. PCR products were purified and quantified by gel extraction. Subsequently, sequencing libraries were constructed, and paired-end sequencing was performed on the Illumina NextSeq 2054 platform.