Helix ligata isolate 141559a RNA-seq
RNA was extracted from posterior foot tissues preserved in RNAlater. Libraries were constructed from poly(A) enriched RNA and sequenced on the NovaSeq X platform. After running rCorrector v1.0.4 to correct likely erroneous k-mers and removing reads flagged as having an unfixable_error, the raw reads were trimmed and filtered with fastp (--adapter_sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCA --adapter_sequence_r2=AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT --trim_poly_g --cut_front --cut_tail --cut_window_size 4 --cut_mean_quality 20 --qualified_quality_phred 15 --unqualified_percent_limit 40 --n_base_limit 5 --length_required 15).