10X Chromium sequencing for assembly of the genome of the grass Festuca ovina
DNA was isolated using a CTAB method described in Stoffel et al., 2012 (BMC Genomics, 13, 185), and cleaned up using Ampure XP beads, following the manufacturer's recommended protocol. DNA quality, quantity and integrity were checked via analysis on the nanodrop platform, via qubit assays, via agarose gel analysis and on the Agilent Fragment Analyzer platform. These analyses indicated that the DNA had high purity and integrity, comprising fragments with length > 60 kbp. The DNA sample was used to create a sequencing library using ChromiumTM Genome Library Kit and Gel Bead Kit v2 (16 reactions, PN-120258). Library preparation date: 2020-01-16.