Small RNA-seq of plasma exosomal RNA from NSCLC patients or non-cancer controls.
Small RNA libraries were prepared from plasma exosomal RNA using the QIAseq miRNA Library Kit (Qiagen, Cat. No. 331502).  The protocol includes 3′ and 5′ adapter ligation, reverse transcription, cDNA amplification with unique molecular identifiers (UMIs), and library cleanup to enrich for mature small RNAs such as miRNAs, piRNAs, and tRNA fragments. The resulting libraries were size-selected to retain small RNA species and made ready for Illumina sequencing.