CT26 HDAC2 (CRX2125767)
Cells were crosslinked in medium containing 10% FBS and 1% formaldehyde for 10 min at room temperature, quenched with 0.125 M glycine for 5 min, and washed with cold PBS. Pellets were sonicated to shear chromatin and centrifuged. Supernatants were diluted 10-fold in IP buffer and incubated with antibodies, followed by overnight binding to Dynabeads Protein G. Beads were washed, and crosslinks. DNA was purified after proteinase K and RNase A digestion using a QIAquick MinElute Kit. ChIP-seq libraries were prepared and sequenced on an Illumina NovaSeq X Plus platform