GSM8896712: Experiment_C,Strain_Dsup,Condition_0mM_H2O2,rep2; Saccharomyces cerevisiae; RNA-Seq
RNA extraction was performed with the MasterPure Yeast RNA Purification Kit (Epicentre MPY03010) using the standard protocol including DNA removal step. RNA quality, concentration and purity was determined by Tapestation (Agilent 5067-5576) and nanodrop (Fisher Scientific), and material stored at -80oC until library preparation. For each biological replicate, three technical replicates of non-strand specific libraries were prepared from 200ng mRNA (Fast RNA-seq Lib Prep Kit v2; Abclonal RK20306)