NGS of Hepatitis B and Hepatitis D Virus: Adult human plasma
HBV and HDV reads were captured from metagenomic libraries via an xGen probe enrichment approach.  Designed probe sets covering HBV genotypes A-I and HDV genotypes 1-8 were combined into one probe set for hybridization. The combined xGen probe set was modified with a 5 biotin tag and Nextera barcoded primers. Hybridization, bead capture, washes, and library amplification were performed. Samples were repeatedly washed to remove unbound sequences, and captured cDNA was pooled and sequenced on an Illumina NextSeq.