GSM9758930: naïve B cells, subject 6, GEX; Homo sapiens; RNA-Seq
RNeasy Micro Kit (Qiagen) as per manufacturer's protocol. RNA was extracted from peripheral blood B cells isolated from blood via Ficoll density centrifugation and then using flow cytometry sorting for CD19+CD27- naïve B cells of varying CD38 expression (high, mid, and low). RNA libraries were prepared using the NEB Ultra Express Kit with Poly-A selection. Separate libraries were used for general gene expression and B cell receptor data.