GSM9469890: End reperfusion with 22 minutes of in situ ischemia, OVX, 1; Rattus norvegicus; RNA-Seq
Snap-frozen left ventricles of all hearts were powdered and 22-30 mg tissue was used for total RNA isolation performed with the RNeasy Plus Mini Kit (Qiagen, Hilden, Germany). The total RNA was further cleaned and DNaseI treated using an RNA Clean & Concentrate-5 kit, DNase included (Zymo Research R1014) according to their protocol. Sequencing libraries were made using an illumina TruSeq Stranded mRNA Library Prep kit (illumina, 20020595) in combination with TruSeq RNA UD Indexes (illumina, 20022371) according to illumina's guidelines.