16S rRNA amplicon seq of anaerobic biomass: anaerobic membrane bioreactor
PCR amplification of targeted regions was performed by using specific primers connecting with barcodes. The PCR products of proper size were selected through 2% agarose gel electrophoresis. The same amount of PCR products from each sample was pooled, end-repaired, A-tailed, and further ligated with Illumina adapters. Libraries were sequenced on a paired-end Illumina platform to generate 250bp paired-end raw reads