Sanger-sequencing of human: proband LMBRD1 locus 1
DNA was extracted from peripheral blood samples using TIANamp Genomic DNA Kit. Primers were designed and PCR was performed to amplify specific regions. PCR products were purified from 2% agarose gel using TIANamp DP214-03 kit. Sanger sequencing was performed using xlAB 3730xL Genetic Analyzer and data was analyzed using DNA Sequencing Analysis 5.2.0 software.