GSM8691190: 32C-1d-HKEC-rep3; Anopheles gambiae; RNA-Seq
RNA was extracted using TRIzol per manufacturer's instructions. RNA was then resuspended in Buffer RLT (Qiagen) containing 1% 2-mercaptoethanol (Fisher Scientific), and re-purified using the RNeasy Mini Kit (Qiagen). The re-purification included DNase treatment in the column, and elution in RNase-free water. Library preparation for sequencing was completed using the stranded mRNA NEBNext Poly(A) mRNA library preparation kit (New England BioLabs) with an input of 300 ng of total RNA.