UCE target enrichment of Gnamptogenys_scimitar_EX1365: adult worker
Samples were extracted using column-based extraction kits. DNA was sheared to 400-600 bp using a DNA sonicator. Genomic libraries were prepared using Kapa Hyper Prep Kits (1/4 volume reactions) and iTru, 8bp dual-indexing adapters (Glenn et al. 2019). Samples were pooled into pools of about 10 samples and enriched for ultraconserved elements (UCEs) using an ant-customized RNA bait set (Arbor Biosciences; ant-specific hym-v2) that includes 9,898 baits (120 mer) targeting 2,524 UCE loci shared across Hymenoptera and a set of legacy markers (Branstetter et al. 2017). Enriched pools were pooled together and shipped to the University of Utah genomics core facility for sequencing on an Illumina HiSeq 2500 instrument (2x125 bp v4 chemistry).