GSM9648451: HEK293T cells, KO, H3K4me1, ChIP; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, Chromatin was sheared to an average length of 300-700 bp with a Qsonica sonicator. ChIP-seq libraries were prepared using VAHTSTM Universal DNA Library Prep Kit for Illumina. After amplification and size selection with DNA clean beads from Vazyme, the size distributions were determined with Agilent 4200 Bioanalyzer.