CS-18-3-16S rDNA
High-throughput sequencing libraries were prepared following TruSeqTM Nano DNA sample preparation Kit from Illumina (San Diego, CA), using 1ug of total DNA. DNA end repair,A-base addition and ligation of the Illumina-indexed adaptors were performed according to Illuminas protocol. Libraries were then size selected for DNA target fragments of ~400bp on 2% Low Range Ultra Agarose followed by PCR amplified using Phusion DNA polymerase (NEB) for 15 PCR cycles. All samples were sequenced by the Illumina NovaSeq 6000 platform (150bp*19, Guangdong MeiG Gene Technology Co., Ltd, shenzhen, China)