GSM8690497: Wt + PBS lung day1_1; Mus musculus; RNA-Seq
We used the right lung stored at −80°C after resection with Allprotect Tissue Reagent (QIAGEN; Venlo, Netherlands, cat. 76405) to purify RNA using the RNeasy Plus Mini Kit (QIAGEN; Venlo, Netherlands, cat. 74134) according to the manufacturer's protocol. The quality and concentration of the RNA were verified using a Qubit fluorometer (Life Technologies, CA, USA) and an Agilent 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA). Purified total RNA (500 ng) was used to prepare RNA libraries using the Quant Seq 3' mRNA-seq library preparation kit FWD of Illumina (LEXOGEN, Vienna, Austria) following the instructions provided by the manufacturer. RNA libraries were sequenced on an Illumina NextSeq 500 system with 75-nt-long reads.