Bulk RNA-sequencing on peripheral blood mononuclear cells (PBMCs) collected from Sjögren's disease cases and healthy controls
Whole blood RNA-seq was collected into PAXGene tubes and extracted with on-column DNase digestion, then RNA quality was assessed using the RNA 6000 Nano kit. Sequencing libraries were prepared using 250ng of RNA, then paired-end 100bp RNA sequencing was performed on the Illumina HiSeq 2000, multiplexing 6 samples per lane. Detailed methods are reported in Joachims, et al. RMD Open 2022. PMID: 36456101. PMC9717416.