GSM9080384: SPARC Knockdown, rep2; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol reagent following the manufacturer's instructions. RNA was DNase-treated and precipitated using sodium acetate and ethanol. RNA integrity was assessed by electrophoresis. Libraries were prepared using the Illumina TruSeq Stranded Total RNA Library Prep Gold Kit with Ribo-Zero, following protocol 1000000040499 v00 (Macrogen).  Raw FASTQ files were aligned to the human reference genome (GRCh38/hg38) using STAR v2.7.