GSM8764070: CART19_VX800_CD4_D03; Homo sapiens; RNA-Seq
RNA extraction RNA was quantified via the Qubit RNA HS Assay Kit (Invitrogen, Q32852), and quality-assessed for integrity via High Sensitivity RNA ScreenTape (Agilent, 5067-5579). 500 ng high-quality RNA with a RIN score > 7 of each sample was used for library preparation using NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs, E7760S). Libraries were prepared according to manufacturer protocols. All libraries were quantified via the Qubit dsDNA HS Assay Kit (Invitrogen, Q32851), quality-checked for fragment sizes via High Sensitivity D5000 ScreenTapes (Agilent, 5067-5592), pooled, and sequenced (Illumina, NovaSeq X).