GSM8579902: Monash sham #2, [Mon_sham_s2206]; Rattus norvegicus; miRNA-Seq
Whole blood was collected under isoflurane anesthesia from the lateral tail vein into K2 EDTA tubes. Plasma was separated by centrifugation at 1300 g for 10 min at 4 °C. RNA was extracted from 200 μl plasma using miRNeasy serum/plasma kit with elution volume of 14 μl. Small RNA library preparation was conducted with QIASeq miRNA library kit. Small RNA-seq. Single-end sequencing of 75-bp reads was performed at 12M depth by Illumina NextSeq 550.