Whole-genome sequencing of Citrobacter amalonaticus: surgical site infection isolate from human patient
Genomic DNA was extracted from a pure culture of Citrobacter amalonaticus isolated from a surgical site infection in a human patient. DNA quality and concentration were assessed using spectrophotometry and fluorometry. A paired-end sequencing library was prepared using an Illumina-compatible protocol involving random fragmentation, end repair, adapter ligation, and PCR enrichment. Sequencing was performed on an Illumina NovaSeq 6000 platform, generating 101 bp paired-end reads.