GSM9613870: PTS_LGG_P60; Mus musculus; OTHER
For spatial transcriptomics, LGG-bearing mouse brains were processed and embedded in paraffin by the Pathology Core at BCM. The brains were sectioned into 6 µm thick coronal sections and attached within the capture area on the Visium slides. Tumor locations were identified using H&E staining. For spatial transcriptomics, The libraries were prepared with the Visium Spatial Gene Expression Reagent Kits (10x Genomics, PN-1000187) and sequenced on an Illumina HiSeq1500 instrument and a paired-end flow cell (High output) according to manufacturer protocol.