Dendrophthoe pentandra small RNA library B1
Small RNA libraries were prepared using the TruSeq Small RNA Kit according to the manufacturer's instructions. Adapter ligation was performed using adapter mix and T4 RNA ligase, followed by cDNA synthesis and PCR amplification. The final libraries were purified by gel purification, and target fragments of 145-160 bp were excised from a Novex 6% TBE gel. Library quality and quantity were assessed using TapeStation and Qubit Fluorometer. Sequencing was performed as 50-cycle single-end reads on the Illumina NextSeq 2000 platform.