GSM8898299: EV-3; Homo sapiens; RNA-Seq
Total RNA was extracted using the miRNeasy Mini Kit (Qiagen, Cat# 217004). Poly(A)+ mRNA was enriched using NEBNext® Poly(A) mRNA Magnetic Isolation Modules. Libraries were prepared using NEBNext Ultra II RNA Library Prep Kit. Sequencing was performed by McMaster Genomics Facility on an Illumina NextSeq 2000 using 2x50 bp paired-end reads, targeting 50 million reads per sample.