RNA-seq of Bos grunniens:subcutaneous fat of castrated yak
Magnetic beads with Oligo (dT) were used to isolate mRNA from total RNA. The mRNA was randomly fragmented into the small fragments of approximately 300 bp using fragmentation buffer. Using reverse transcriptase, the first-strand cDNA was synthesized with mRNA as the template, followed by second-strand synthesis. End Repair Mix was added to convert the double-stranded cDNA into blunt ends, followed by the addition of an A base to the 5' ends. The adapter-ligated products were purified and size-selected, and the size-selected products were subjected to PCR amplification.