GSM9380101: 06L_Rapa; Mus musculus; RNA-Seq
RNA was extracted from liver with TRI Reagent (Thermo Fisher, AM9738), then treated with DNase (Invitrogen, 12185010) and purified with RNA purification kit (Ambion, 12183025). The concentration and purity of RNA was determined using a NanoDrop 2000c spectrophotometer (Thermo Fisher Scientific, Waltham, MA) and RNA was diluted to 100-400 ng/µl for sequencing. The RNA was then submitted to the University of Wisconsin-Madison Biotechnology Center Gene Expression Center & DNA Sequencing Facility, and RNA quality was assayed using an Agilent RNA NanoChip. RNA libraries were prepared using the TruSeq Stranded Total RNA Sample Preparation protocol (Illumina, San Diego, CA) with 250ng of mRNA, and cleanup was done using RNA Clean beads (lot #17225200).