Whole exome sequencing of patient-derived preclinical models: Sample 467112-017-R-ORIGINATOR-DNA
"Whole exome libraries were generated by shearing 50 ng genomic DNA to 150-180 bp using Covaris LE220 sonicator (Covaris Inc., Woburn, MA). Sheared genomic DNA was processed using KAPA HyperPrep library construction and dual index kit through end-repair, dA tailing, ligation with indexed Illumina adaptors and subsequent PCR amplification to enrich adapter-attached fragments.  Amplified libraries were hybridized with a biotinylated RNA bait set of human exome panel (SureSelectXT Human V5, Agilent Technologies Inc.).  The captured genomic DNA fragments were enriched using streptavidin-coated magnetic beads and amplified to generate final whole exome libraries. The final libraries were quantified, pooled and sequenced on Illumina sequencing platform."