HP_GFP2_R1 (CRX1753269; CRA026415)
Total RNA was isolated and purified from root tissue samples using TRIzol reagent, with >1 microg of total RNA meeting the requirements for downstream experiments. PolyA-tailed mRNA was specifically captured using oligo(dT) magnetic beads. The captured mRNA was fragmented at elevated temperature and subjected to paired-end sequencing (PE150) on an Illumina NovaSeq 6000 platform (LC Bio Technology Co., Ltd., Hangzhou, China) following standard operating procedures.