RNA-Seq of mus musculus: adult female spleen
Here is the professional English translation: Total RNA was first extracted from the samples using the Trizol method, and the RNA quality was assessed using a Thermo NanoDrop One and an Agilent 4217 TapeStation. After passing quality control, ribosomal RNA was removed using the Epicentre Ribo-Zero rRNA Removal Kit, and strand-specific libraries were constructed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina.