GSM8969550: 4DNEX8YC3ET9, H. sapiens - K562, condition2 - conditionAI - anti-Lamin B1, B1 T1; Homo sapiens; OTHER
H1, HCT116, and HFF cells were cultured according to standard operation protocols established by the NIH 4D Nucleome Consortium (https://www.4dnucleome.org/cell-lines.html). K562 cells were cultured according to the ENCODE Consortium protocol (http://genome.ucsc.edu/ENCODE/protocols/cell/human/K562_protocol.pdf). SON and MKI67IP TSA-Seq was performed using Condition E (labeling with 1:300 tyramide biotin, 50% sucrose and 0.0015% hydrogen peroxide) (Zhang et al., 2020) with the following minor modification: 150ul of Dynabeads M-270 streptavidin (Invitrogen, catalog no. 65306) was used to purify the biotinylated DNA. For LMNB1 TSA-Seq, Condition A2 (Zhang et al., 2020) (labeling with 1:10000 tyramide biotin, 50% sucrose, 0.0015% hydrogen peroxide and reaction time 20 min at RT) was used.