GSM9542070: EMU-SI-M1; Mus musculus; RNA-Seq
Approximately 3-cm-long distal ileal and 2cm proximal colonic tissue fragments were harvested beginning 1 cm upstream and downstream from each cecal junction. Tissues were immediately placed in Buffer RLT (Qiagen) on ice and homogenized using a gentleMACS Octo Dissociator (Miltenyi Biotec). RNA isolation and purification were performed using the RNeasy Mini Kit (Qiagen, cat. 74104), following manufacturer's instructions. Bulk RNA library preparation was performed by the University of Wisconsin-Madison Biotechnology Center's Gene Expression Center using the Illumina TruSeq Stranded mRNA kit for poly(A) enrichment.