K4_60-h(iEFC) (CRX1095220; CRA016958)
In this study, embryonic stem cells were treated with the chemical cocktail K4 series, and samples were collected at various time points to examine the effects of the treatment. The 96-h cells were used for chemical induced embryo model generation. Single-cell RNA sequencing was performed on cells at 24 hours, 60 hours, and 96 hours post-induction. Additionally, single-cell RNA sequencing was conducted on ci-MOs at 1.5 days, 2.5 days, and 4.5 days after in vitro culturing.