GSM9685485: 786-O,Dark_1; Homo sapiens; RNA-Seq
Total RNA was isolated from treated 786-O cells using TRIzol reagent following the manufacturer's instructions. RNA quality, concentration, and purity were evaluated before subsequent library preparation and RNA-seq analysis. RNA-seq library construction was performed by Sangon Biotech (Shanghai, China) according to their standard library preparation protocol. Total RNA samples that passed quality control were used for cDNA library preparation and subsequent high-throughput sequencing.