GSM8464529: HCT116, H3K4me1-ChIP; Homo sapiens; ChIP-Seq
HCT116 cells were crosslinked with 1 % formaldehyde for 10 min at room temperature and quenched with 125 mM glycine. The fragmented chromatin fragments were precleared and then immunoprecipitated with Protein A + G Magnetic beads coupled with anti-H3K4me1 antibodies. After reverse crosslinking, ChIPed and input DNA fragments were end-repaired and A-tailed using the NEBNext End Repair/dA-Tailing Module (E7442, NEB) followed by adapter ligation with the NEBNext Ultra Ligation Module (E7445, NEB). Sequencing libraries were generated by Genefund Biotech (Shanghai, China). The DNA libraries were amplified for 15 cycles and sequenced using Illumina Hi-Seq with 150 bp paired-end protocol.