CRISPRt in A. baumannii_sigmas library_sigAb-gene-complement-strain-native-promoter
CRISPRt (targeted Tn-seq) of sigAb operon and Aba sigmas - to test gene essentiality - in A. baumannii ATCC 17978 background containing sigAb gene complementation with native sigAb promoter; methods: mated cells at 30C, plated on selection plates at 30C, scraped off plates, prepped gDNA, mmeI digested and followed Tn-seq library prep protocol