GSM9760371: IR_3; Mus musculus; RNA-Seq
Total RNA was extracted using the TRIzol reagent (Invitrogen) according to the manufacturer's instructions. RNA integrity was assessed using an Agilent 2100 Bioanalyzer, with RIN ≥ 7.0 required for library construction. mRNA was enriched using poly‑T oligo‑attached magnetic beads. The purified mRNA was fragmented and reverse‑transcribed into cDNA. A‑tailing and adapter ligation were performed, followed by PCR amplification to generate the final paired‑end RNA‑seq libraries. Libraries were sequenced on the Illumina NovaSeq 6000 platform (150 bp paired‑end reads).