RNA-seq of rat pheochromocytoma PC-12 Replicate 2: Treatment Catechin+CORT
Qualified genomic DNA was firstly sonicated randomly, and then end-repaired, A-tailed, and adaptor ligated using NEBNext LtraTM DNA Library Prep Kit for Illumina (NEB, USA) according to the preparation protocol. DNA fragments with length of 300-400 bp were enriched by PCR. At last, PCR products were purified using AMPure XP system (Beckman Coulter, Brea, CA, USA) and libraries were analyzed for size distribution by 2100 Bioanalyzer (Agilent, Santa Clara, CA) and quantified using real-time PCR. Genome sequencing was performed on the Illumina Novaseq 6000 sequencer using the pair-end technology (PE 150).