Illumina NovaSeq 6000 paired end sequencing of SAMD00901850
Genomic DNA was fragmented using a Covaris system to obtain an average insert size suitable for paired-end 500 bp sequencing. Library preparation was performed using the TruSeq DNA PCR-Free Library Prep Kit (Illumina), following the manufacturer's instructions. After end repair and adapter ligation, size selection was conducted by excising DNA fragments of the desired size from an agarose gel. The final libraries were quantified and quality-checked prior to sequencing. Sequencing was performed using the NovaSeq 6000 Reagent Kit (500 Cycles).