Ribo-seq of quadriceps muscle tissues from 10 months old, 30 months old, 30 months old calorie restricted, 30 months old rapamycin treated adult mice
Ribo-seq of quadriceps muscle tissues from 10 months old, 30 months old, 30 months old calorie restricted, 30 months old rapamycin treated adult mice
Mice were sacrificed using CO2 inhalation and muscle were extracted and snap freezed immediately. All procedures were performed in accordance with Swiss regulations for animal experimentation, and approved by the veterinary commission of the Canton Basel-Stadt Hot phenol extraction of RNA SMARTer smRNA-Seq Kit for Illumina, Takara Bio