GSM9896391: Patient09, Grade 4, IDH1 mutant, Primary; Homo sapiens; RNA-Seq
Total RNA was extracted using Trizol® Reagent (Invitrogen, USA) according to the instruction manual. Purified RNA was quantified at OD260nm by using a ND-1000 spectrophotometer (Nanodrop Technology,USA) and qualitated by using a Bioanalyzer 2100 (Agilent Technology, USA) with RNA 6000 LabChip kit (Agilent Technologies, USA). All RNA Sample preparation procedures were carried out according to the official Illumina protocol. Agilent's SureSelect Strand-Specific RNA Library Preparation Kit were used for library construction followed by AMPure XP Beads size selection.