BCR-Seq of Mus musculus adult female breast tumor and draining lymph node samples
B cell receptor sequencing was performed on B cells isolated from breast tumors and draining lymph nodes of adult female BALB/c mice at 14 and 21 days post tumor inoculation. B cells were sorted by FACS using CD19, B220, and CD138 markers. Libraries were prepared by amplifying immunoglobulin heavy chain variable regions and sequenced on an Illumina MiSeq platform (2300 bp).