GSM8726468: M1; Mus musculus; RNA-Seq
Total RNA was isolated using RNeasy plus micro kit (Qiagen). Quantity of total RNA was assessed with the Qubit 2.0 and quality was checked using a RNA 6000 Pico chip on Agilent's bioanalyzer. Total RNA (RIN>8) was sent to Novogene (Cambridge, UK) for library preparation and sequencing (PE150) on a NovaSeq 6000 (Illumina).