GSM9348309: ABX replicate 3; Mus musculus; RNA-Seq
Harvested tissue were immediately placed into TRIzol (Invitrogen) after mouse euthanasia. All samples were subsequently snap frozen on dry ice and stored at -80°C until use. Prior to starting RNA extraction, samples were thawed on ice and homogenized with approximately six to eight ceramic beads (2.8 mm; OMNI International) using the Bead Ruptor 12 (OMNI International). The Bead Ruptor was programmed to the highest speed for 3 rounds of 15 seconds. Subsequent chloroform extraction was utilized on the homogenized samples. The RNeasy PLUS Mini Kit (Qiagen) was used for RNA isolation and purification according to manufacturer's instructions. Extracted RNA was submitted to Psomagen for QC, Library Prep, and bulk RNA-sequencing. Briefly, TruSeq stranded mRNA library kit was used and pair-end sequencing was performed using the Illumina NovaSeq X platform, generating approximately 50 million paired-end reads per sample.