GSM9024517: Hepatocyte-like cells, Etoposide, Day5; Homo sapiens; ATAC-seq
According to NovoGene's in-house protocols, nuclei was extracted from samples. The nuclei pellet was resuspended in the Tn5 transposase reaction mix. The transposition reaction was incubated at 37°C for 30 min. According to NovoGene's in-house protocols, equimolar Adapter1 and Adatper 2 were added after transposition, PCR was then performed to amplify the library. After the PCR reaction, libraries were purified with the AMPure beads and library quality was assessed with Qubit.