GSM9436294: FHC,sh-METTL1-3; Homo sapiens; RNA-Seq
Total RNA was extracted from FHC cells using TRIzol reagent according to the manufacturer's instructions. RNA concentration and integrity were assessed before library preparation. RNA sequencing libraries were constructed using the Illumina TruSeq RNA Library Preparation Kit according to the manufacturer's instructions. Briefly, poly(A)+ RNA was enriched from total RNA, fragmented, and reverse-transcribed to generate cDNA libraries, which were subsequently indexed and amplified for paired-end sequencing.