GSM8691040: H139-0032-T5_S3_L001; Homo sapiens; OTHER
DNA was extracted using a QIA-cube TRB libraries were amplified from genomic DNA, using the EuroClonality two-step TRB assay, developed by the Euroclonality-NGS Working Group (manuscript in preparation), aiming for 1μg of PBMC DNA input per sample. After each PCR step, amplification success was checked using the D1000 DNA ScreenTape assay on a 4200 Agilent TapeStation (Agilent, Santa Clara, CA, USA) and libraries were purified using the AMPure XP kit (Beckman Coulter, Brae, CA, USA.