GSM9317685: Streptomyces coelicolor A3(2) parB1-3xFLAG replicate 2; Streptomyces coelicolor; ChIP-Seq
Cells were sonicated in lysis buffer and pellted. Supernatant was incubated with anti-FLAG antibody beads at 4°C overnight. DNA was then isolated from the beads. Libraries were prepared for Illumina sequencing using the NEXT Ultra II library preparation kit (NEB) according to the manufacturer's protocol