Whole-genome sequence of strain HC1M1C21
Genomic DNA extracted with the Maxwell RSC Cultured Cells DNA Kit (Promega) after enzymatic lysis (lysozyme/mutanolysin). Library prepared with the Oxford Nanopore Native Barcoding Kit (SQK-NBD114). Sequenced on an Oxford Nanopore MinION device with an R10.4 flow cell (FLO-MIN114) using MinKNOW. Duplex base-calling and demultiplexing performed with Dorado v0.9.2.