GSM8464778: COLO320DM_ChIA-PET-3%HD-rep1; Homo sapiens; OTHER
Cells were crosslinked with 1% FA and 2mM EGS according to the ChIA-PET protocol (PMC8351913). Cells were lyzed and chromatins were fragmentized with Alu I enzymes (R0137L, NEB) at 37 ˚C.  After overnight digestion, digested nucleus was then subjected to proximity ligation overnight at 16 ˚C. The ligated chromatins were sonicated and immunoprecipitated by anti-RNAPII antibodies (920102, Biolegend). The immunoprecipitated DNAs were then tagmented and linker-conjucated fragments were enriched using streptoavidin-beads. Libraries were prepared and sequenced as previously described (PMC8119378). The immunoprecipitated DNAs were then tagmented and linker-conjucated fragments were enriched using streptoavidin-beads. Libraries were prepared and sequenced as previously described (PMC8119378).