GSM9649324: AC014; Mus musculus; RNA-Seq
Extravascular and intravascular leukocytes from heart tissue were isolated via flow sorting following heart digestion by Liberase TM. Single-cell RNA-seq and TCR libraries were generated using the 10X Genomics Chromium OCM platform with 5′ mRNA and TCR sequencing according to the manufacturer's protocol. OCM samples were multiplexed and sequenced across two lanes for both gene expression (GEX) and TCR libraries. FASTQ files are lane-specific and contain pooled reads from multiple samples. Sample identity was determined using Illumina index reads and 10x Genomics cell barcodes, and FASTQ files from both lanes corresponding to each OCM sample were combined for downstream analysis with Cell Ranger with outputs per sample