Transcriptomic analysis of nuclear receptor(LXR, FXR) modulation, mTOR inhibition, and nutrient stress on human HepG2 cells
Transcriptomic analysis of nuclear receptor(LXR, FXR) modulation, mTOR inhibition, and nutrient stress on human HepG2 cells
HepG2 cell line was ordered from ATCC (HB-8065) and cultured. Cells were harvested directly from cell culture and no dissection was perormed. HepG2 cells were cultivated in DMEM (Gibco, 41965) containing 10% FBS (Gibco, 10500064) and 1% penicillin/streptomycin (Gibco, 15140122) Cells were treated with treated with GW3965 (5µM), GW4064 (5 µM) alone or combined with mTORC1 inhibitor Torin 1 (TOI), or combined with glucose/glutamine starved media (GLUST). DMSO with final concentration of 0.1% (v/v) was used as control treatment.  RNA was extracted using Trizol reagent (Invitrogen, 15596026) according to the manufacturer's instructions. QuantSeq 3' mRNA library preparation kit (Lexogen) according to manufacturer's instructions.  