GSM8578283: single cells from control 3 mice; Mus musculus; RNA-Seq
The tissue was incubated in a digestion buffer consisting of 0.1% collagenase II (Gibco 17101015), 0.2% collagenase IV (Worthington LS004189), and 50 μg/ml DNase I in a 5% FBS-PBS solution at 37 °C for 80 minutes on a shaker. After centrifugation, the pellets were digested using 0.25% trypsin at 37 °C for 10 minutes. A single-cell suspension was obtained by filtering through 70-μm and 40-μm filters, followed by centrifugation at 500g at 4 °C for 10 minutes Library preparation was performed using 10x Genomics Chromium Next GEM Single Cell 5' Kit v2 reagents according to the manufacturer's instructions