Sample2
Total RNA was isolated from frozen mouse stool day 6 samples as described7. Briefly, lysis and disruption steps were performed with 1:1 mix of 1mm acid-washed glass beads and 0.1 mm zircona beads using a bead beater for efficient lysis and disruption; Precellys24 (Bertin Instruments Cat # EQ03119-200-RD000.0). RNA purification was further performed    according to the manufacturers protocol using mirVana miRNA isolation kit, with phenol (Fisher Scientific AM1560). RNA eluted was treated with Turbo-DNA-free kit to remove any DNA impurities (Fisher Cat # AM1907)