RNA-Seq of Isa textula late instar venom spines
Tissue was stored in RNAlater reagent (Ambion). Total RNA was extracted from the venom spines and surrounding tissue using TRIzol (Thermo Fisher) according to the manufacturers instructions, with volumes reduced for smaller starting tissue masses. Poly-(A)+ enrichment and TruSeq library construction was performed by The University of Queenslands Institute for Molecular Bioscience (IMB) Sequencing Facility using either a NextSeq or HighSeq sequencer (Illumina).