GSM9109237: HG00731, SMC1 HiChIP, DMSO; Homo sapiens; OTHER
The HiChIP experiment was performed using Arima-HiC+ Kit (HiChIP User Guide for Mammalian Cells and Tissues using Transcription Factors and Histones (A101020) with modifications. Cell fixation was carried out according to FA-EGS cross-linking (Buka et al., 2025). Chromatin immunoprecipitation step (ChIP) was performed with 10 μg of SMC1 (Bethyl Laboratories, A300-055A). Library preparation was performed as described in the Arima-HiC+ Kit HiChIP Library Preparation using Swift Biosciences Accel-NGS 2S Plus DNA Library Kit protocol.