GSM8465188: Wildtype mouse 1, Gne+/+, C3+/+, Sequencing Run 1; Mus musculus; RNA-Seq
RNA was harvested from retinal whole-mounts that were homogenized in QIAzol Lysis reagent (Qiagen), afterwhich chloroform was used to separate the RNA phase and isopropanol to precipitate the RNA. Afterwards, the suspension was washed with 70% ethanol and the final pellet was resuspended in ultra-pure DEPC treated water. QuantSeq 3′ mRNA-Seq Library Prep Kit, Lexogen with an input of 100 ng total RNA