RNA-Seq of Homo sapiens: neutrophil from adult male peripheral blood
Total RNA was extracted from PMN using the TRIzol reagent (Invitrogen, CA, USA) according to the manufacturers protocol. RNA purity and quantification were evaluated using the NanoDrop 2000 spectrophotometer (Thermo Scientific, USA). RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, USA). Then the library was constructed using VAHTS Universal V6 RNA-seq Library Prep Kit according to the manufacturers instructions. The library was sequenced on an llumina Novaseq 6000 platform and 169 bp paired-end reads were generated.