GSM9760891: UNX/DOCA, ITF2357 Chow, rep 4; Mus musculus; RNA-Seq
Total RNA was collected from ~10 mg of left ventricular tissue from four biological replicates using the Direct-zol RNA Miniprep Kit (Zymo Research R2051) according to the manufacturer's instructions. Following mRNA selection using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs E7490S), cDNA libraries were prepared from 1 µg of mRNA using the NEBNext Ultra Directional Library Preparation Kit for Illumina® (New England Biolabs E7420S) according the manufacturer's protocol.