GSM9081646: IWP2_DIV40 n2; Homo sapiens; RNA-Seq
Midbrain organoids were dissociated into single-cell suspension following the Worthington Papain Dissociation System kit instructions (Worthington Biochemical) with intermittent trituration steps. The final solution was centrifuge 300g per 7 min, cell pellet were resuspended in PBS-0,04% BSA and filtered twice (40um, 30um) to obtain single-cell suspensions. Cells loaded to Chromium Chip (target cell recovery): 10,000 cells. Library Preparation Kit: Chromium Next GEM Single Cell 3ʹ Reagent Kit v3.1 (10x Genomics). Sequencing parameters: NovaSeq X, SP Flow Cell, 100 cycles. Read 1: 28 bp, i7-index: 10 bp, i5-index 10 bp, Read 2: 90 bp.