GSM8969002: Human FLI1CUT&Tag IgG, Rep3; Homo sapiens; OTHER
Nuclei were harvested using nuclear extraction buffer (20mM HEPES, 10mMKCl, 0.1% Triton X-100, 20% glycerol, protease inhibitor [Sigma 11836170001], 0.5mM spermidine) CUT&Tag libraries were constructed as previously described in Cheng et al., 2023.