16s RNA-Seq of colon content
PCR products were quality-checked using 2% agarose gel, purified with AMPure XP Beads (Beckman, USA), and quantified with Qubit 3.0. Libraries were prepared using Illumina DNA Prep Kit (Illumina, USA) and qualified with ABI StepOnePlus Real-Time PCR System. Qualified libraries were sequenced on Novaseq 6000 using PE250 mode.