GSM8691126: Control, heart; Mus musculus; RNA-Seq
Cardiac tissues were harvested from mice and washed in pre-cooled PBSE (PBS buffer containing 2 mM EGTA). Nuclei isolation was performed using GEXSCOPE® Nucleus Separation Solution (Singleron Biotechnologies, Nanjing, China). The isolated nuclei were resuspended in PBSE. The concentration of single nucleus suspension was adjusted to 3-4 × 105 nuclei/mL in PBS. The single nucleus suspension was then loaded onto a microfluidic chip (GEXSCOPE® Single NucleusRNA-seq Kit, Singleron Biotechnologies), and snRNA-seq libraries were constructed according to the manufacturer's instructions (Singleron Biotechnologies). Sequencing was performed on an Illumina novaseq 6000 instrument with 150 bp paired end reads.