Kinase activity of RIOK2 transports FADDRIPK1Caspase-8 complex from lysosome to ER and triggers spatial cleavage of GSDMD
Total RNA was isolated, its concentration, RIN value, and 28S/18S fragment size were measured, used for RNA-seq analysis. cDNA library construction and sequencing were performed by Beijing Genomics Institute using BGISEQ-500 platform. RNA libraries were prepared for sequencing using standard Illumina protocols