GSM8606102: ATG5 KO SEAM biol rep 1; Homo sapiens; RNA-Seq
Total RNA extraction from cells was performed using TRIzol reagent followed by DNase treatment using the RNase-free DNase set. The amount of input RNA is determined by the quantity of RNA samples. Ilumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus kit is used for the construction of NGS libraries from the samples with acceptable quality parameters.