GSM8578070: islets from FXR knockout mice,female,3months; Mus musculus; RNA-Seq
The total RNA of each group of islets was extracted separately and the RNA purity was detected to meet the requirements After the total RNA extracted from the sample was treated with DNase I, the eukaryotic mRNA was enriched with magnetic beads with Oligo (dT).The interrupting reagent was added to break the mRNA into short fragments, and a strand of cDNA was synthesized with six-base random hexamers using the interrupted mRNA as a template, and the second strand of cDNA was synthesized by adding buffer, dNTPs and DNA polymerase I.After kit purification and recovery, sticky end repair, adding base "A" to the 3 'end and connecting the sequencing connector, the obtained fragment was amplified and enriched by PCR after size selection.