GSM8464657: JURKAT, day7, unsorted, rep1; Homo sapiens; OTHER
Cells were lysed in buffer ATL (Qiagen) and decrosslinked overnight at 56C. gDNA was purified using QIAamp DNA Blood Midi Kit (Qiagen). sgRNA fragments in gDNA were amplified in 2 PCR reactions with a maximum of 35 cycles in total using NEBNext® High-Fidelity 2X PCR Master Mix. Sample barcodes with a stagger region were added in the second PCR. Libraries were gel extracted and purified using Agencourt AMPure XP beads (Beckman Coulter) validated using the Agilent High Sensitivity DNA kit.