Oxford Nanopore mixed adaptive sampling and shotgun sequencing of Wolbachia from Aedes aegypti (Tw-Kao line)
Whole genome sequencing was performed using Oxford Nanopore sequencing with a mixed configuration. Half of the sequencing pores were configured for adaptive sampling enrichment targeting the Wolbachia reference genome, while the remaining pores performed standard whole genome shotgun sequencing. Genomic DNA was extracted from whole Aedes aegypti mosquitoes and sequenced without prior Wolbachia enrichment.