P13_Dura_MPAS_1
The genomic DNA was isolated from peripheral blood and fragmented by Frag enzyme (MGI, Shenzhen, China) to produce DNA fragments between 100 and ~1,000 bp, suitable for paired-end read length of 150 bp (PE150) sequencing according to the manufacturer's instructions (MGI FS DNA library prep set, cat No. 1,000,005,256). The fragmented DNA was further selected to be between 300 and ~500 bp using DNA clean beads (MGI, Shenzhen, China). A dTTP-tailed adapter sequence was ligated to both ends of the DNA fragments. The ligation product was then amplified for 19 cycles and subjected to the following single-strand circularization process. The PCR product was denatured to obtain a single-strand circular DNA library