Typha minima_guo_L2_UDI332. (CRX1830455; CRA027597)
Strand-specific mRNA sequencing libraries prepared using Illumina TruSeq Stranded mRNA LT Sample Prep Kit (polyA selection). Total RNA extracted from stem tissue was enriched for polyadenylated transcripts using oligo-dT magnetic beads. Enriched mRNA was fragmented (median insert size: 350 bp), reverse-transcribed into cDNA with dUTP-based strand marking, end-repaired, A-tailed, and ligated with Illumina adapters. Libraries amplified with 15 PCR cycles. Final size selection performed using AMPure XP beads. Sequencing on NovaSeq 6000 in PE150 mode.