RNA-Seq of Solanum lycopersicum: control and bacterial spot-inoculated leaves
Total RNA was extracted from tomato leaves using the DP432-RNA prep pure plant kit (Tiangen). RNA concentration and purity were assessed using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA), and RNA integrity was further verified using the Agilent 2100 Bioanalyzer. Sequencing libraries were constructed using the standard Illumina protocol, which included mRNA isolation via Oligo(dT)-attached magnetic beads, fragmentation, and cDNA synthesis. The libraries were sequenced on the Illumina NovaSeq 6000 platform with a 150 bp paired-end (PE150) reading strategy. A minimum of 5.81 Gb of clean data was obtained for each of the 18 samples, with Q30 ratios exceeding 93.07%