Lycium barbarum L: FL-cDNA of root
mRNA was enriched by Oligo (dT) magnetic beads and then synthesized first-strand cDNA by reverse transcription using a Clontech SMARTer PCR cDNA Synthesis Kit (Clontech, Mountain View, CA, USA). The double-strand cDNA was amplified by downstream large-scale PCR reactions after optimization of the PCR cycles. BluePippin Size Selection System (Sage Science, Beverly, MA) was used to select size of the PCR products larger than >4 kb, which were mixed in equal amounts with unselected fragments. SMRTbell libraries were constructed using SMRTBell Template Prep Kit (Pacific Biosciences, Menlo Park, CA) and sequenced on the PacBio Sequel platform.