GSM9686653: Household contact 1200469 (L2, Converter); Homo sapiens; RNA-Seq
Venous blood (2.5 mL) was collected at index patient TB diagnosis into PAXgene Blood RNA Tubes (PreAnalytiX), before initiation of TB treatment. Total RNA was extracted using the PAXgene blood miRNA kit (Qiagen) with semi-automated extraction on the QIAcube (Qiagen). RNA quality and quantity were assessed using the LabChip GX HiSens RNA system (PerkinElmer). RNA-seq libraries were prepared from total RNA using the TruSeq RNA Sample Preparation Kit v2 (Illumina) at the University Medical Center Groningen (UMCG), Groningen, the Netherlands. Samples were sequenced on an Illumina HiSeq 2000 (instrument ID SN163, run 653, flowcell BC6D3JACXX) as single-end 101 bp reads. Samples were demultiplexed by barcode and distributed across 6 lanes of a single flowcell, with approximately 10 samples per lane.