RNA-seq of A549-ACE2-control:sample2
Total cellular RNA of control and UHRF1 sgRNA-edited A549-ACE2 cells was extracted using the RNAsimple Total RNA Kit (Tiangen #DP430) according to the manufacturers instructions. Library preparation was performed by Novogene, Inc., using at least 0.5 g high-quality total RNA input per sample. mRNA enrichment was performed using poly-T oligo-attached magnetic beads. Sequencing libraries were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) following the manufacturers recommendations. Libraries were sequenced on an Illumina Hiseq 2500 platform to generate paired-end reads.