RNA sequencing of CRC cells
total RNA was extracted using TRIzol (Invitrogen). RNA quality (RIN  7.0) was confirmed before library construction with the NEBNext Ultra II RNA Library Prep Kit (NEB). Libraries were sequenced on an Illumina NovaSeq 6000 (150 bp paired-end). Clean reads processed with fastp were aligned to the human genome (GRCh38) using STAR, and gene counts were obtained with featureCounts. Differential expression was analysed with DESeq2 (|log2FC|  1, FDR < 0.05). GO and KEGG enrichment of DEGs was performed using clusterProfiler.