Eight effector KO
Toxoplasma gondii cultures were grown on Human Foreskin Fibroblasts in 12 T12.5cm2 flasks. Parasites were fully egressed and filtered through a 22um filter and spun down at 700g for 7 min at 4C. Cells were then washed in 1mL PBS and spun down at 2000g x 2. Pellet was frozen. DNA was extracted using the QIAamp DNA Blood Mini Kit from Qiagen, with RNase A (10mg/mL) added to the lysis step. DNA was eluted in 40uL TE buffer for a final concentration of 116.7 ng/uL.