GSM9060573: A2, LOAD, Normal_HTT; Homo sapiens; miRNA-Seq
Total RNA was extracted using the mIRNeasy Micro Kit (Cat. No 74004, QIAGEN) following the manufacture´s protocol. 5 mg of tissue was used for RNA extraction. Small RNA-Seq was performed by Seqplexing (Sequencing Multiplex S.L., Spain) using 200 ng of caudate nucleus total RNA per sample for miRNA library preparation. Library quality was assessed using the QIAxcel Advanced System (QIAGEN). Sequencing was performed on an Illumina NovaSeq X platform (Illumina, USA), generating paired-end reads (2 x 150bp). The sequencing depth ranged from 8.64 to 144 million reads per sample (median = 25.89 million).