Amplicon sequencing of Escherichia coli: LasR binding site library, replicate 1, FACS bin 8
Sort-seq of a randomized LasR binding site library in Escherichia coli DH10B. Cells carrying the plasmid-borne variant library were sorted by FACS into eight fluorescence bins and the binding site region was amplified by PCR from each sorted bin separately, then sequenced on an Illumina platform. Reads submitted here are the per-bin reads after primer removal. This sample corresponds to one fluorescence bin of one biological replicate.