GSM9075688: hMP, donor1, CRISPRa, rep1, CRISPR guide capture; Homo sapiens; OTHER
Sorted cells were resuspended in 1X PBS with 0.04% BSA. A total of 20,000 cells per sample were loaded and library preparation was performed according to the manufacturer's protocol (10x Genomics, CG000206 Rev D) using the 10x Genomics Chromium Single Cell 3' Gene Expression v3.1 kit with Feature Barcode technology (10x Genomics PN-1000079), generating both gene expression and CRISPR guide capture libraries.