16S rRNA V3-V4 bacterial amplicon sequencing of high-pressure bioreactor R2 at 40 bar, replicate a
Bacterial 16S rRNA V3-V4 hypervariable region amplified using primers 357wF (5'-CCTACGGGNGGCWGCAG-3') and 785R (5'-GACTACHVGGGTATCTAATCC-3'). Library prepared following Illumina 16S Metagenomic Sequencing Library Preparation protocol with two PCR amplification steps (35 cycles initial PCR + index PCR). Paired-end 300 bp sequencing on Element AVITI platform using avidity chemistry.