GSM9542314: Xenobot, before stimulus, sample 3; Xenopus laevis; RNA-Seq
A sample of Xenobot consisted of 50 pooled Xenobots and each was repeated 3 times. Total RNA was extracted from tissue using TRI Reagent (Molecular Research Center, Inc - MRC) as per the manufacturer's protocol, and total RNA quality and quantity was assessed using a NanoDrop spectrophotometer (Thermo Fisher Scientific). RNA library preparation was done with the Illumina Stranded Total RNA with Ribo-Zero Plus. Libraries were then multiplexed, and an rRNA depletion run using single-end, 75-nucleotide sequencing was performed on NextSeq 550 High Output 75 cycles.