GSM9263639: Day 60 iPSC-derived spinal cord organoid; Homo sapiens; RNA-Seq
Day-60 human iPSC-derived spinal cord organoids were dissociated into single cells by incubation with papain (20 U/ml) at 37 °C for 60 min. Following enzymatic digestion, the cell suspension was washed with a DNase–protease inhibitor solution and centrifuged at 300 g for 5 min. The resulting single-cell pellet was immediately used for 10x Genomics single-cell RNA-sequencing library preparation. scRNA-seq libraries were prepared with the Chromium Next GEM Single Cell Fixed RNA Sample Preparation kit 10x Genomics P/N 1000414) as per manufacturer's protocol. Library prep and sequencing were performed by the Australian Genome Research Facility, and data were generated with the Illumina BCL Convert 4.1.5 pipeline.