DNA samples collected from cow dung
Genomic DNA was extracted from cultured tachyzoites of the Toxoplasma gondii RH strain and quantified. Sequencing libraries were prepared using the Nextera DNA Flex kit (Illumina) with dual indexing as per the manufacturer's instructions. The final libraries were quality-controlled and quantified before being pooled and sequenced on an Illumina NovaSeq 6000 to generate 150 bp paired-end reads.