GSM8464800: PC3DM_ChIATAC-vector-merged; Homo sapiens; OTHER
Cells were crosslinked with 2% FA and 2mM EGS. Cells were lyzed and chromatins were fragmentized with Alu I and HpyCH4V enzymes at 37 ˚C.  After overnight digestion, digested nucleus was then subjected to proximity ligation overnight at 16 ˚C. The nucleus were then tagmented and linker-conjucated fragments were enriched using streptoavidin-beads. Libraries were prepared and sequenced as previously described (PMID: 36639381).