Staphylococcus aureus strain 09Dec2015-G-165
The isolate was taken from a milk sample collected following aseptic teat end preparation and submitted for routine microbiology following the procedures outlined by the National Mastitis Council*. The isolate was confirmed as S. aureus on the basis of being gram-positive cocci, catalase positive and positive on a latex agglutination test (BD BBL Staphyloslide Latex Test Kit, Becton Dickinson, Franklin Lakes, NJ, USA). The isolate was submitted for further testing on Dorset egg slopes (Fort Richard Laboratories, Auckland, New Zealand) and re-cultured on Columbia horse blood (CHB) agar plates (Fort Richard Laboratories). From a pure sub-culture, a heavy inoculum was made in nutrient broth No. 2 (Oxoid, Hampshire, UK) containing 15% glycerol and an aliquot frozen at -80oC. A sub-culture was made on CHB agar and a single bacterial colony selected for DNA extraction using a QIAamp DNA Mini Kit (QIAGEN, Hilden, Germany). DNA libraries were prepared using the Nextera XT DNA preparation kit (Illumina, San Diego, CA, USA) for submission to New Zealand Genomics Limited (University of Otago, Dunedin, New Zealand), where 2 x 100 base pair sequencing was performed on the Illumina NextSeq 500 platform.