GSM9107600: Zebrafish intestinal cells at 5 dpt; Danio rerio; RNA-Seq
At 4 dpf, DEAB treated tg(phox2bb:GFP) zebrafish with a total colonic HSCR phenotype were selected. In total 277 intestines of 5 days post-fertilization (dpf) larvae were isolated, cells were dissociated using 2.17mg/mL papain and the live cells were sorted using FACS. Cells were then loaded onto the 10X platform for single-cell RNA sequencing. Libraries were made according to the manufacter's instructions (Single Cell 3' v3 chemistry, 10x Genomics)