GSM9790918: P53/PTEN KD,N-Myc (ChIP), rep1; Mus musculus; ChIP-Seq
Cell were collected and fixed using formaldehyde to cross-link and preserve protein/DNA interactions. DNA was then sheared by sonication followed by immunoprecipitation using N-Myc antibody (Active Motif, Cat.#61185) and H3K27ac antibody (ab4729). Cross-linking was then reversed, and ChIP DNA was purified. ChIP-Seq libraries were generated from the ChIP-DNA using a custom Illumina library type on an automated system (Apollo 342, Wafergen Biosystems/Takara). ChIP-Seq libraries were sequenced on Illumina NextSeq 500 as 75-nt single end reads. Adapter sequences were not trimmed during demultiplexing.