CK363_1 (CRX1577136; CRA024142)
RNA was extracted using the TruSeqTM RNA Sample Preparation Kit (Illumina, San Diego, CA) with 1microg of total RNA. Messenger RNA was isolated using the polyA selection method with oligo(dT) beads and fragmented. Double-stranded cDNA was synthesized using a SuperScript kit (Invitrogen, California, USA) with random hexamer primers. The cDNA underwent end-repair, phosphorylation, and 'A' base addition, followed by size selection for 300 bp fragments on a 2% agarose gel. The libraries were amplified using Phusion DNA polymerase (NEB) for 15 PCR cycles, then sequenced on the Illumina HiSeq xten/NovaSeq 6000 platform, generating paired-end reads of 150 bp.