Y2H-seq of Mj-ISE6 interaction screen  selective media pool Fw
This study uses yeast two-hybrid sequencing (Y2H-seq) to identify protein interactors of the Meloidogyne javanica effector Mj-ISE6. The Mj-ISE6 bait was expressed in the Saccharomyces cerevisiae PJ69-4A strain and super-transformed with a high-complexity Solanum lycopersicum cDNA library. Colonies growing on selective medium (SD-LWH + 3-AT) were pooled, and plasmid DNA containing prey inserts was extracted. Prey fragments were PCR-amplified and sequenced using Illumina HiSeq 2000 paired-end 125 bp sequencing. Sequencing data were used to quantify prey abundance and identify candidate interactors enriched under selective growth conditions.