RNA-seq of HLA-A2 restricted human CD8+ T cells against SARS-CoV-2 from 11 COVID-19 patients
RNA-seq of HLA-A2 restricted human CD8+ T cells against SARS-CoV-2 from 11 COVID-19 patients
HLA-A2+/peptide pentamer and tetramer selection from patient PBMCs 10x Genomics 3' v3.1 protocol. In brief, after cell sorting, single-cell suspensions were loaded into the Chromium controller to make nanoliterscale droplets with uniquely barcoded 3' gel beads called GEMs (gel bead-in emulsions). After GEM-RT, GEMs were cleaned up by Dyna beads MyOne Silane beads (Thermo Fisher Scientific, 37002D). According to the manufacturer's instructions, the cDNA was amplified and size-selected by SPRI-beads (Beckman Coulter, B23317). Finally, the 3' transcript and feature barcode libraries were pooled 10x Genomics 3' v3.1 protocol. In brief, after cell sorting, single-cell suspensions were loaded into the Chromium controller to make nanoliterscale droplets with uniquely barcoded 3' gel beads called GEMs (gel bead-in emulsions). After GEM-RT, GEMs were cleaned up by Dyna beads MyOne Silane beads (Thermo Fisher Scientific, 37002D). According to the manufacturer's instructions, the cDNA was amplified and size-selected by SPRI-beads (Beckman Coulter, B23317). Finally, the 3' transcript and feature barcode libraries were pooled