Metagenomic sequencing of dental wastewater
Plasmidsaurus: We sequence each sample with Oxford Nanopore long reads to very high depth before generating an assembly using the latest super-accurate basecalling model and polishing software:    We construct an amplification-free long-read sequencing library using the newest v14 library prep chemistry, including minimal fragmentation of the input genomic DNA in a sequence-independent manner via tagmentation.    We sequence the library with a primer-free protocol using the most accurate R10.4.1 flow cells (raw data is delivered in .fastq format).We use the latest flowcells and chemistry kits from Oxford Nanopore, along with the latest Super Accurate basecalling model. Genome assembly accuracy is typically around Q50-60, which corresponds to 99.999%, or one error per 100,000 bases, to 99.9999%, or one error per 1,000,000 bases.