CK_1 (CRX1593322; CRA024412)
Total RNA was extracted from the tender leaves of Zanthoxylum nitidum using the Plant RNA Purification Reagent kit (Biomarker Technologies, Beijing, China). Using Oligo (dT) magnetic beads for mRNA enrichment, followed by the synthesis of the first strand cDNA guided by random hexamer primers, and the construction of double stranded cDNA through a dUTP-based strand marking method. Purified products were amplified by PCR to construct sequencing libraries with insert sizes >200 bp.