GSM8464275: P1 cortex CUT&Tag-seq of histones [H3K27ac_S36]; Mus musculus; OTHER
The hGFAP-GFP mice were sacrificed at E18 or P1. The green brain dorsal cortices were collected, minced and dissociated into a single-cell suspension using a Papain Cell Dissociation Kit by following the manufacturer's instruction (Miltenyi Biotec, catalog no. 130-092-628). The GFP+ cells were captured and harvested through fluorescence-activated cell sorting (FACS). Paired-end Illumina sequencing was performed on an Illumina NovaSeq platform following the manufacturer's instructions and produced 150 bp paired-end reads. All raw sequence data were quality trimmed to a minimum Phred score of 20 using trim_galore version 0.6.7. Apparent PCR duplicates were removed using Picard MarkDuplicates vl.107. All paired-end reads generated from the CUT&Tag-Seq of pERK and GLI3 were aligned to the mm39 mouse genome using subread-align version 2.0.6. The removal of obvious PCR duplicates and filtering of BAM files were achieved through sambamba markdup version 0.8.2 and samtools view version 1.13, respectively.