GSM8764109: G1-ESCtoEpiLC-RAD21_EpiLC_DMSO_rep2-batch1; Mus musculus; RNA-Seq
Total RNA was extracted from 500,000 cells per sample using RNeasy Mini Kit (Qiagen, 74104), following the manufacturer's instructions. For M-to-G1 experiments, RNA-seq libraries were generated by Weill Cornell Medicine Genomics Resources Core Facility using NEBNext Ultra II Directional RNA library kit (New England Biolabs, E7760) and TruSeq Stranded mRNA library kit (Illumina, 20020595). For asynchronous experiments, RNA-seq libraries were generated by Novogene using their mRNA library preparation service with poly A enrichment.