GSM9437051: Cortical neurons derived from 2L20 iPSCs with heterozygous CHMP2B G26403C mutation, biological replicate 2; Homo sapiens; ATAC-seq
ATAC-seq DNA was isolated as described previously (Grandi et al. 2022, Nature Protocols) with the following modifications. 1x105 cells were used to generate each library. Tagmentase (Diagenode) and home-made 2x TD Buffer (20 mM Tris-HCl pH 7.5, 10 mM MgCl2, 20% dimethylformamide) were used in place of the previously described TDE1 enzyme and 2x TD Buffer from Illumina. After tagmentation, DNA was isolated using the Qiagen MinElute PCR Purification Kit. Libraries were generated from isolated DNA essentially as described previously (Grandi et al. 2022, Nature Protocols). Libraries were amplified with 10 cycles of PCR, and fragments between 150 and 500 bp were gel purified using the Qiagen Gel Extraction Kit.