GSM9685369: RNAseq_Kdm6bWT_p30_rep6; Mus musculus; RNA-Seq
RNA was extracted and purified from cells using QI shredder (Qiagen) and RNeasy (Qiagen) spin columns. Total RNA was extracted using per the manufacturer's instructions. RNA-seq libraries were  repared from total RNA using the VAHTS Universal V10 RNA-seq Library Prep Kit for Illumina (Vazyme). Adapter-ligated cDNA was PCR-amplified and size-selected magnetic beads. DNA was measured by an Invitrogen Qubit fluorometer. Libraries were sequenced on AVITI or NovaSeq 6000 sequencer at the Michigan State University Genomics Core.