GSM9315930: Sample P24051_1007 scRNA; Homo sapiens; RNA-Seq
Nuclei were isolated following the 10x Genomics Chromium Next GEM Multiome ATAC+GEX protocol. Cells were washed, lysed with ATAC Nuclei Isolation Buffer containing RSB, NP-40 and Tween-20, and nuclei were cleaned and resuspended in Nuclei Buffer. Chromium Next GEM Single Cell Multiome ATAC + Gene Expression libraries were generated using 10x Genomics reagents. ATAC transposition and reverse transcription were performed in GEMs. Libraries were constructed, amplified, and indexed according to the manufacturer's protocol.