GSM9469722: iPSC Small Intestine Organoid, PEGstiff, Mesenchymal, Rep k; Homo sapiens; RNA-Seq
Sorted EPCAM+ and CD90+ populations were lysed in RLT buffer (Qiagen) supplemented with β-mercaptoethanol. Samples were sent to Azenta for RNA extraction using their standard pipeline. Libraries were prepared using a standard RNA-seq workflow (Azenta). Sequencing was performed on an Illumina NovaSeq 6000 instrument to a depth of ~30 million paired-end reads per sample (2x150 bp configuration).