GSM9262965: HCV non-infected, long RNA, batch 2, rep5; Homo sapiens; OTHER
Briefly, in the modified RAP-RNA protocol, RNA molecules in close proximity were cross-linked using UV365 in the presence of the psoralen molecule, 4′-aminomethyltrioxsalen (AMT). Cells were then fractionated into nuclear and cytoplasmic constituents, and cytoplasmic viral RNA was enriched using pull-down with specific DNA probes. The enriched RNA was then DNAse treated to remove the DNA probes, and reverse cross-linked using UV254. To allow efficient identification of both short and long RNA interactors, two parallel strand-specific RNA-library methods were applied, based on poly-adenylation and template switching (CATS small-RNAseq-kit (Diagenode)), or random hexamer priming (NEBnext Ultra II), respectively.