CUT&RUN of SpenW522 mutant and wildtype ESCs
CUT&RUN was performed using Cell Signaling CUT&RUN Assay Kit #86652. Antibody used was Cell Signaling Technology Acetyl-Histone H3 (Lys27) (D5E4) XP Rabbit mAb #8173. DNA was purified using Cell Signaling DNA Purification Buffers and Spin Columns #14209. For library prep, NEBNext Ultra II DNA Library Prep Kit for Illumina was used.