GSM9109122: Jurkat, sgRNA1 (S1), Clone A (CA), No Trail, 4sU, biol rep 3; Homo sapiens; OTHER
6 h post-TRAIL treatment, total RNA was extracted in the dark using TRIzol (Invitrogen) and phenol-chloroform extraction. ERCC Spike-In (Invitrogen) and mixed 4sU unlabelled/labelled spike-in was added. RNA was then alkylated, DNase-treated, and cleaned. General protocol from Lexogen's SLAM-seq Anabolic labelling kit. RNA libraries for SLAM-seq/RNA-seq were prepared using KAPA RNA Hyperprep Kit with RiboErase (HMR) with KAPA Unique Dual Index Adaptors according to the manufacturer's protocols and include ERCC Spike-In mix for normalization. Sequencing on NovaSeq X Plus 25B.