GSM9613670: WT.35.CD40.IL.4; Mus musculus; RNA-Seq
Total RNA was extracted from primary splenic B cells by GENEWIZ (Azenta Life Sciences) using standard RNA isolation protocols, followed by quality assessment using Bioanalyzer prior to library preparation. RNA-seq libraries were prepared by GENEWIZ (Azenta Life Sciences) using Illumina-compatible protocols. Briefly, mRNA was enriched from total RNA, fragmented, and reverse transcribed to cDNA, followed by adaptor ligation and PCR amplification. Libraries were sequenced on an Illumina HiSeq platform to generate paired-end 150 bp reads.