GSM8579386: RiboSeq, EPP2 cells, Ctu1KO, DMSO, replicate5; Mus musculus; OTHER
RNA was harvested by Trizol-chloroform extraction and isopropanol precipitation. Cytoplasmic RNA from ribosome protected fragments (RPFs) were isolated from cytosolic cell lysate. Isolates were RNaseI-treated  and monosome were purified using TRIzol reagent. Libraries were constructed according to McGlincy NJ and Ingolia NT.  Methods. 2017