GSM8579021: ES-2-MC2-Liver cell, in_vitro_culture_7.doubling, mini-CRISPR library, R3; Homo sapiens; OTHER
Cell pellets were dissolved by STE buffer, proteinase K and SDS and incubated at 65℃ overnight. Saturated NaCl solution and chloroform were used for phase separation, followed by isopropanol for DNA precipitation. DNA pellets were then washed twice by 70% ethanol solution and dissolved by ultra-pure water. 30μg DNA per sample was used for subsequent library construction. NGS library construction was followed to Feng Zhang's protocol in 2017.