GSM9759609: IRF4; Mus musculus; OTHER
scRNA-seq: Mouse P14 Cas9 control transduced or transduced with a sgRNA targeting IRF2, IRF4 or IRF8 were flow sorted from B16-gp33 tumors in Cas9-expressing host mice 14 days after tumor engraftment and 7 days after adoptive co-transfer of control and KO. Single cell suspensions from each tumor were labelled with a hashtagging antibody prior sorting and sequencing (APC-Streptavidin-oligonucleotide conjugates, Biolegend). CUT&RUN: Post antibody incubation, samples were processed following manufacturer's instruction (EpiCypher) scRNA-seq: Chromium Single Cell 5' Kit v2 (Dual Index) - 5'RNA-seq CUT&RUN: Libraries were prepared following the manufactures instruction (EpiCypher) with the modification of increasing the concentration of the beads to ensure smaller fragments were not lost during the purification process. Libraries were pooled and subjected to 3 further rounds of bead purification to exclude the adapter dimers present in the libraries.