C2C12 Myotubes, Day-3 rep1 Polysome RNA-seq
The total RNA extracted from the polyribosome fractions of C2C12 myoblast and C2C12 myotube samples were rRNA depleted, fragmented, and the cDNA library was prepared using the Invitrogen혲 Collibri혲 Stranded RNA Library Prep Kit for Illumina혲 with Collibri혲 H/M/R rRNA Depletion Kit. The libraries were sequenced for 100nt  paired-end reads on the Illumina NovaSeq 6000 platform using NovaSeq 6000 SP Reagent Kit v1.5.