RNA-seq of SH-SY5Y treated 7-OHMG replicate 3
NGS libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following the manufacturers instructions. Poly(A) mRNA was isolated using the NEBNext Poly(A) mRNA Magnetic Isolation Module. Fragmentation and priming were followed by first and second-strand cDNA synthesis. The resulting double-stranded cDNA was end-repaired, A-tailed, and ligated to Illumina adapters. Libraries were size-selected (~360 bp) and amplified via PCR (11 cycles). Final libraries were assessed using an Agilent 2100 Bioanalyzer and quantified with a Qubit 2.0 Fluorometer before being pooled for sequencing