GSM9685178: Collagen, wounded, 72 hrs; Mus musculus; RNA-Seq
Collagen gels were digested with collagenase (10 mg/mL, Fisher Scientific) for 10 minutes, and a Dead Cell Removal kit (Miltenyi Biotec) was used according to the manufacturer's instructions to deplete dead cells. The resulting single-cell suspension was loaded onto the 10x Genomics Chromium Controller for single-cell droplet generation, cell lysis, and RNA barcoding. Single-cell RNA-Seq libraries were prepared and sequenced on an Illumina NovaSeq instrument using 100-cycle paired-end (PE) sequencing.