GSM8690381: W20_PTH_3; Mus musculus; RNA-Seq
Total RNA was isolated from cells using the RNeasy Mini Kit (Qiagen Germantown, MD, USA) according to the manufacturer's protocol. 500ng of total RNA was used for the construction of sequencing libraries Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library.