NC (CRX1819511; CRA027283)
Each cell/nuclus was involved into a droplet which contained a gel bead linked with up to millions oligos (cell unique barcode). After encapsulation, droplets suffer light cut by MobiNovaSP-100(MobiDrop, cat. no.A2A40001) while oligos diffuse into reaction mix. The mRNAs were captured by gel beads containing oligo(dT) in droplets. Following reverse transcription, cDNAs with barcodes were amplified, and a library was constructed using the High Throughput Single Cell 3'RNA-Seq Kit v2.0 (MobiDrop (Zhejiang) Co., Ltd., cat. no. S050200201) and the 3' Single Index Kit( MobiDrop (Zhejiang) Co., Ltd., cat. no. S050300201).