GSM9685474: mPASMC_Normoxia_1; Mus musculus; ncRNA-Seq
Total RNA was extracted from the mPASMCs using TRIzol reagent according to the manufacturer's instructions. Small RNA libraries were prepared using the Pandora-seq strategy. Briefly, RNA samples were enzymatically treated to remove RNA modifications that interfere with adapter ligation and reverse transcription, followed by standard small RNA library construction procedures.