GSM9709376: Non-proliferating CD4⁺ T-cells, No PCS,HC5; Homo sapiens; RNA-Seq
After 6 days of stimulation and PCS exposure, CD4 proliferated cells were sorted by BD FACSAria™ II (BD Biosciences), followed by RNA isolation using the RNeasy Kit (catalog 74104, QIAGEN, Venlo, NL) and library preparation. Library preparation was performed according to the manufacturer's instructions using the Illumina® Stranded Total RNA Prep with Ribo-Zero Plus Kit (catalog 20040529, Illumina, San Diego, CA, USA), and IDT for Illumina® RNA UD Indexes Set A, Ligation (96 Indexes, 96 Samples, catalog 20040553). cDNA library quality was assessed using an Agilent High-Sensitivity DNA Kit (catalog 5067-4626, Agilent Technologies, Santa Clara, CA, USA), yielding a broad peak between 300-800 base pairs.