scRNA-Seq of Mus musculus (C57BL/6): NTM-infected lung, male, 6 months
High-quality single-cell suspensions (without prior sorting of specific cell populations) were loaded into the MobiNova-100 Single-Cell System and processed according to the MobiCube Single-Cell 3 RNA-seq Kit protocol for cell capture (targeting 5,000 cells), cDNA synthesis, amplification, and library preparation. Libraries were sequenced on the Illumina NovaSeq 6000 platform using 150 bp paired-end multiplexing runs, targeting a minimum sequencing depth of 20,000 reads per cell to ensure sufficient coverage for downstream analyses.