GSM9435803: WE Rapa, Biol rep 3; Saccharomyces cerevisiae; RNA-Seq
Cells were centrifuged and treated with 2U zymolyase for 30 minutes at 37°C. RNA was extracted using the E.Z.N.A. Total RNA Kit I (OMEGA), according to the manufacturer's instructions. Subsequently, the samples were treated with DNase I (Promega) to eliminate genomic DNA contamination. RNA integrity was confirmed using a Fragment Analyzer (Agilent). RNA-seq libraries were prepared using the Illumina TruSeq Stranded mRNA protocol following the manufacturer's instructions.