16S: microbial depletion in Exaiptasia pallida
16S gene sequencing was performed on symbiotic and aposymbiotic Exaiptasia pallida to understand microbiome recovery following antibiotic depletion (C = control, 14 days in FSW; R0/A = 7 days in ABS; R2/P = 7 days in ABS + 2 days in FSW; R7/R = 7 days in ABS + 7 days in FSW). Metabarcoding libraries were generated using a series of PCR amplifications for the V4/V5 region of the bacterial 16S rRNA gene. Five negative controls were prepared and used to remove contaminating sequences (not provided). Samples were sequenced on an Illumina Miseq at Tufts University Core Facility (paired-end 250 bp).