GSM9685236: SARS-CoV2_NSP14-mut passage 14 in VERO-NP cells; Severe acute respiratory syndrome coronavirus 2; RNA-Seq
Culture supernatants from serial passages of recombinant SARS-CoV-2 in Vero-NP or Vero-AT cells were processed for RNA extraction using TRIzol LS reagent (Invitrogen) according to the manufacturer's instructions. The extracted RNA was converted into sequencing libraries and subjected to paired-end deep sequencing (100 or 150 bp reads) with NovaSeq 6000 system