GSM8796421: FIS x luh 2DAI rep1; Arabidopsis thaliana; RNA-Seq
Three biological replicates for each genotype in 4 different time points were prepared. Flowers were collected and immediately frozen in liquid nitrogen. The tissue was homogenized with the Precellys Homogenizer and the RNA was isolated with the QIAwave RNA Mini Kit (Qiagen). DNA was removed by DNase I digestion followed by LiCl precipitation. The RNA concentration was measured using the Qubit 4 (Invitrogen) and the RNA integrity was assessed on a 4200 TapeStation (Agilent). Samples with an RNA integrity number equivalent (RINe) higher than 6 were considered for further processing. Total RNA (300 ng) served as input for library preparation using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina). After library preparation, samples were run on the 4200 TapeStation (Agilent) for quality control.