RADseq: Cryophytum
The Adapterama III methodology (Bayona-Vasquez et al. 2019) was used to prepare RADseq libraries. We used the ClaI and EcoRI iTru i5 and iTru i7 internal indexes. Restriction digestion used the MspI read 1 and EcoRI-HF read 2 enzymes, and the ClaI read 1 adapter dimer-cutting enzyme. Further details are provided in the associated publication: "Using population genetics to match invasive Mesembryanthemum nodiflorumpopulations in North America to their origin" by Clarke J.M. van Steenderen, Emma Sandenbergh, Dean Brookes, Patrick J. Moran, Massimo Cristofaro, William F. Hoyer III, and Iain D. Paterson