GSM8635307: Arabidopsis_shoot_hda6_ros1-4_Repeat_1; Arabidopsis thaliana; ATAC-seq
The extracted nuclei were incubated with Tn5 transposase and tagmentation buffer according to the manufacturer's instructions (Vazyme, TD501-01). After tagmentation, DNA was purified using the MinElute PCR Purification Kit (QIAGEN, 28004). PCR amplification of the library was performed for 10 cycles under the following conditions: 72 °C for 5 min, 98 °C for 30 s, followed by thermocycling at 98 °C for 10 s, 60 °C for 30 s, and 72 °C for 40 s, with a final extension at 72 °C for 5 min. After PCR amplification, the libraries were purified using VAHTS DNA Clean Beads (Vazyme, N411) and sequenced on an Illumina NovaSeq X Plus platform.