Targeted DNA-seq of Homo sapiens: adult urothelial carcinoma
Genomic DNA from fresh/FFPE tumor tissues (Shanghai cohort) and matched blood (Streck tubes, processed within 72h) was sheared to 200 bp (Covaris E210). Libraries were prepared using Accel-NGS 2S DNA Library Kit with xGen Lockdown Probes (custom exon/intron probes), quantified via Qubit 3.0 and Agilent 2100 Bioanalyzer. Paired-end sequencing (2150 bp) was performed on Illumina NovaSeq 6025.