GSM9898245: Aβ42-treated forebrain organoid with maraviroc treatment and iPSC-derived microglia co-culture rep 2, cDNA library; Homo sapiens; RNA-Seq
Following completion of the experimental treatments, forebrain organoids were dissociated into single-cell suspensions using an enzymatic dissociation protocol. Cell suspensions were filtered through a 40-μm cell strainer, and cell viability was assessed prior to library preparation. Qualified single-cell suspensions were immediately processed for single-cell RNA sequencing. Single-cell suspensions were processed using the MGI DNBelab C-TaiM4 platform. Individual cells were encapsulated with mRNA capture magnetic beads containing unique cell barcodes and unique molecular identifiers (UMIs) using a microfluidic droplet system. Cell lysis occurred within droplets, and poly(A)+ mRNA molecules were captured by Oligo(dT) primers on the magnetic beads and reverse transcribed to generate barcoded cDNA. The cDNA was purified, amplified, and ligated with sequencing adapters to construct sequencing libraries. Library quality was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies, USA). Gene expression (cDNA) libraries and oligo libraries were sequenced on a DNBSEQ-T7 platform in paired-end 100-bp mode (PE100). Sequencing was performed by Hangzhou Astrocyte Technology Co., Ltd.