HiC-Seq of Sorghum bicolor:P184
High-molecular-weight genomic DNA from young leaf tissue of Sorghum bicolor inbred line P184 was extracted using a CTAB-based protocol, quality checked (Qubit, OD ratios, Femto Pulse), and used to prepare a PacBio HiFi SMRTbell library with >15 kb size selection for Sequel IIe sequencing. A Hi-C library (formaldehyde crosslinking, MboI digestion) was prepared and sequenced (Illumina PE150) for scaffolding.