TPO-2 (CRX1237596; CRA019936)
Primary neurons were seeded into 6-well plates and cultured for 24 h. 200ng TPO was added 2 h before OGD. The culture medium was then replaced with glucose-free DMEM with 200 ng TPO to initiate glucose deprivation. Subsequently, the plates were incubated anaerobically for 6 hours using the AnaeroPack system to induce OGD. Afterwards cells were collected for RNA-seq.