GSM9078466: PRS CK STORM exosomes, Production batch 4 (miRNA); Homo sapiens; miRNA-Seq
Total exosomal RNA was extracted from PRS CK STORM using the exoRNeasy Maxi Kit (QIAGEN), following the manufacturer's instructions. Small RNA libraries were prepared using the QIAseq miRNA Library Kit (QIAGEN), incorporating UMIs during reverse transcription. Libraries were PCR-amplified (22 cycles), indexed, purified, quality-checked (TapeStation D1000), and pooled at equimolar concentrations based on insert size and qPCR quantification.