GSM8690616: Non tranformed calli, rep 2; Cynara cardunculus; RNA-Seq
Total RNAs from calli were extracted in triplicates from three experimental groups: one WT, and two independent transgenic lines carrying the construct pGWB411: SAD (SAD-OE) and pHellsgate12 :: FAD2:2 (FAD-KD), for a total of 9 samples. RNA extraction was performed on100 mg of callus, ground under liquid nitrogen, lysed/homogenized with 1 mL of TRIzol (Thermo Fisher Scientific, #15596026) and, after addition of 0.2 mL of Phenol:Chloroform:Isoamyl-alcohol (25:24:1) and centrifugation (10 min, 13,000× g), the RNA was precipitated from the aqueous phase with 1 volume of isopropanol. Preliminary analysis confirmed that all samples passed the quality control (A260/A280 = 2.10, total RNA amount > 10 µg/sample and RNA integrity number > 9). Illumina TruSeq RNA Sample Prep kit