Sequencing of small RNAs from Rosa hybrida Radrazz buds in response to sugar metabolism
Total RNA was extracted from rose bud samples after 24 hours of in vitro incubation under different sucrose and metabolic inhibitor treatments. Small RNA libraries were prepared using a standard DNBSEQ small RNA library construction protocol. Briefly, small RNAs were size-selected, followed by sequential 3' and 5' adapter ligation, reverse transcription, and PCR amplification. The resulting libraries were purified, quality-checked, and sequenced on the DNBSEQ platform to generate single-end reads.