M5075_2 (CRX1871612; CRA028243)
The mRNA with polyA structure in the total RNA was enriched by Oligo(dT) magnetic beads, and ion interruption was used to break the RNA to a fragment of about 300bp in length. The fragments of 300 bp were selected to construct libraries of 450 bp, and the libraries were quality checked by Agilent Bioanalyzer, and sequenced by Next-Generation Sequencing (NGS) based on the Illumina sequencing platform, and then sequenced by Paired-end (PE). end (PE) sequencing based on the Illumina sequencing platform.