RNA-seq of native heart valves: alcified aortic valve disease
Heart valve fragments were homogenized in TRIzol, and total RNA was extracted with DNase treatment, followed by quality and quantity assessment using Agilent Bioanalyzer 2100, NanoDrop 2000, and Qubit 4 Fluorometer. Poly(A)+ mRNA was isolated, converted into barcoded libraries, quality-checked, quantified by qPCR, and sequenced on the MGIseq-2000.