RNA-Seq
mRNA was enriched from total RNA using Oligo dT magnetic beads. The mRNA was fragmented and reverse-transcribed into first-strand cDNA using random hexamer primers, followed by second-strand synthesis. After end repair, A-tailing, adapter ligation, size selection, and PCR amplification, the sequencing library was constructed. Library quality was assessed using Qubit and Bioanalyzer for concentration and fragment size distribution. Sequencing was performed on the DNBSEQ-T7 platform.