GSM9540970: Female low protein (LP, F), RNAseq, rep 3; Mus musculus; RNA-Seq
RNA was extracted from a quarter of the left kidney that was stored in RNAlaterTM Stabilization solution using TRIZOL (Invitrogen™, 15596018).  The RNA was cleaned with RNeasy MinElute Cleanup Kit (Qiagen, 74204) with RNase-Free DNase Set (Qiagen, 79254). The RNA was reconstituted with RNase-free water to a final volume of 50 μl and stored at – 80 degrees.  RNA concentration and quality were measured with a NanoDrop™ One/OneC Microvolume UV–Vis Spectrophotometer (Thermo Fisher Scientific) and RIN were obtained from the Genome Analysis and Technology Core at UVA using an Agilent 4200 TapeStation system.  Bulk RNA-Seq was performed by Novogene. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.