RNA-seq of PHBV plastisphere microbiome: PHBV_BHT7m
Total DNA and RNA were co-extracted from the plastisphere microbiome using ZymoBIOMICS DNA/RNA Miniprep Kit. were constructed using a TruSeq stranded mRNA library Prep kit with TruSeq RNA CD Indexes excluding the poly-A mRNA enrichment and rRNA depletion step. The library was paired-end sequenced (150 bp 2) on the Illumina HiSeq X platform.