GSM9742278: Peripheral blood mononuclear cells (PBMCs), CS, cecal slurry (CS)-induced polymicrobial sepsis; Mus musculus; RNA-Seq
Whole blood collected in EDTA tubes was diluted 1:1 with PBS and layered onto separation medium. After centrifugation at 500 g for 30 minutes, the PBMC layer was collected, washed, and treated with red blood cell lysis buffer. VAHTS Universal V8 RNA-seq Library Prep Kit