GSM9436219: DMS-MaPseq analysis of in vitro refolded T4 td intron, Control, Replicate #3; Escherichia phage T4; OTHER
Reactions were quenched by addition of 1 volume 1 M DTT, and RNA was purified on Monarch® Spin RNA Cleanup columns (10 μg) as per manufacturer instructions. To prevent carryover of other species that might have formed during the folding/splicing process, the probed RNA was further resolved on a 5% TBE-Urea polyacrylamide gel. The product corresponding to the full-length unspliced RNA was excised and recovered by passive diffusion at 4°C for 16 h in 300 μl 0.05% SDS, supplemented with 60 U SUPERase•In™ RNase Inhibitor.