Whole genome sequencing of Vibrio parahaemolyticus strain H4
Genomic DNA was fragmented to approximately 300500 bp using a Covaris M220 ultrasonicator. Libraries were prepared from 1 g genomic DNA by end repair, 3 adenylation, indexed adapter ligation, size selection, and eight cycles of PCR enrichment. The libraries were quantified, pooled, and sequenced on the DNBSEQ-T7 platform using a 2  150 bp paired-end strategy.