GSM8515166: control group 1; Mus musculus; Bisulfite-Seq
Sperm DNA was extracted with a phenol-chloroform mixture, precipitated with ethanol, dried and dissolved in TE buffer. Sperm DNA was purified using Allprep DNA/RNA mini kit (Qiagen) and was used for the construction of RRBS libraries. RRBS libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina following manufacturer's protocols.