Genome sequence of Leptospira isolates: 4A MS
Genomic DNA was isolated from bacterial cultures containing a minimum density of $10^8$ cells/mL using the Illustra Bacterium GenomicPrep Mini Spin Kit (Cytiva) according to manufacturer protocols. Sample integrity was verified via electrophoresis on 0.8% agarose gels, while purity and concentration were assessed using a NanoDrop spectrophotometer and Qubit Fluorometer. Whole-genome sequencing was subsequently performed at the National Laboratory for Scientific Computing (LNCC, Brazil) utilizing an Illumina NextSeq 2000 system to generate $2 \times 150$ bp paired-end reads. Raw reads were processed with FastQC and Trimmomatic for quality control and trimming. De novo assembly was carried out using SPAdes with default parameters, removing contigs shorter than 500 bp, with assembly quality assessed by QUAST and annotation performed via the NCBI Prokaryotic Genome Annotation Pipeline.