GSM8969404: BCR-seq of mouse lung tissue (Group C); Mus musculus; OTHER
Lung tissues containing visible nodules were collected. Samples were mechanically and enzymatically dissociated into single-cell suspensions using collagenase and hyaluronidase digestion. Single cells were filtered, counted, viability assessed, and immediately used for single-cell capture and library preparation. Single-cell RNA-seq, TCR/BCR immune repertoire, and Hashtag libraries were prepared using the BD Rhapsody system following the manufacturer's protocols. Libraries were sequenced on the Illumina NovaSeq 6000 platform using paired-end sequencing.