Vibrio anguillarum CS13: genome sequencing (ONT)
Isolate was collected from the ventral surface of the lateral spine of the crab with a sterile swab and incubated in marine agar on 30oC for 24 h. Genomic DNA was extracted using MasterPure Complete DNA and RNA Purification Kit (MC85200, LCG Biosearch Technologies, Teddington, UK). DNA was purified using the GenElute-E Single Spin DNA Cleanup Kit (EC600-50RXN; Sigma). Sequencing was performed using the MinION Mk1C (ONT, Oxford, UK) with the Native Barcoding Sequencing Kit (SQK-NBK114.24; version NBE_9196_v114_revQ_15Sep2022). The library was sequenced for 48 hours using FLO-MIN106 R9 flow cell connected to MinKNOW software (v24.02.08; ONT)