16S amplicon sequences from Phragmites australis-associated metagenome: lower leaf
The 16S rRNA V3V4 region was amplified with primers 341F/805R (Herlemann et al., 2011) using metagenomic DNA extracted from the indicated plant tissues. PCR products were purified with AMPure XP beads (Beckman Coulter), indexed with Nextera XT dual indices (Illumina), and cleaned again with AMPure XP. Libraries were sequenced on an Illumina NextSeq 2000 (P1 600cycle kit; 2 x 300 bp) with a 40% PhiX spikein at the Louisiana State University Cancer Research Center Translational Genomics Core.