GSM9263480: Male_Control; Mus musculus; RNA-Seq
The tissues were homogenized, and the nuclei were extracted using 10X Genomics Chromium nuclei isolation kit following manufacturer protocol (10X Genomics, Pleasanton, CA). The nuclei suspension was used as input for automated microfluidic single nuclei capture and barcoding using the 10X Genomics Chromium IX platform. snRNA-seq libraries were generated using Chromium Next GEM Single Cell 5′ Gene Expression v2 kit (10X Genomics) according to the manufacturer's protocol. Single-nuclei gel beads in emulsion (GEMs) were generated and single nuclei were uniquely barcoded. cDNA was recovered and selected for using DynaBead MyOne Silane Beads (Thermo Fisher Scientific) and SPRIselect beads (Beckman Coulter). The constructed library underwent quality control assessment using a High-sensitivity d1000 DNA screentape (Agilent) and then quantified with a Qubit Fluorometer (Thermo Fisher Scientific) and with a Kapa Library Quantification Kit (Kapa Biosystems) by using the AriaMX instrument (Agilent).