GSM8464634: CCRF-CEM, H3K27ac, HiChIP; Homo sapiens; OTHER
Cells were fixed with 1% formaldehyde for 15 min at RT with rotation. After quenching and cell lysis, chromatin was digested using MBol restriction enzyme. Overhangs were filled with biotin-dATP, dCTP, dGTP, dTTP and ligated with T4 DNA ligase followed by sonication using a Covaris S220 Sonicator. Immunoprecipitated DNA was captured by biotin pull-down, transposed with Tn5 and amplified. Post amplification libraries were purified using Agencourt AMPure XP beads (Beckman Coulter) and validated using the Agilent High Sensitivity DNA kit.