GSM8898345: B16Nid1 CD11b_cells 2; Mus musculus; RNA-Seq
Frozen pieces of tumors (approximately 5-8 mg) were mechanically disrupted with metallic beads on TissueLyser LT (Qiagen, Netherlands). CD11b+ cells were isolated by magnetic separation in a Automacs Pro Separator.  Then, total RNA was extracted from  samples using NucleoSpin RNAII kit (Macherery-Nagel). For sequencing total bulk-tumor, mRNA-seq libraries were generated according to the manufacturer's instructions of the TruSeq stranded mRNA Library Prep kit (20020595, Illumina). For sequencing CD11b+ cells, mRNAseq libriaries were generated according Novogene protocols and sequenced on Illumina Novaseq X platform