RAD-Seq data from Pandalus borealis (northern shrimp).
Double digest RAD sequencing libraries were constructed by digesting whole genomic DNA with Sau3AI and ApeKI and selecting fragments 390 bp to 430 bp using a Pippin Prep (Sage Science). Molecular identification tags were assigned to individuals using combinatorial barcoding of forward (5bp) and reverse (6bp) reads, respectively, and pooled into a library before size selection and amplification using 10 PCR cycles. Resulting library concentrations were quantified using SYBR Gold double-stranded DNA assay measured on a TECAN GENios plate reader (TECAN, www.tecan.com), and the sizes estimated by 2% agarose gel. Libraries were sequenced at Novogene UK, using Illumina HiSeq 2500 (paired-end 2  125 bp) on a single lane