GSM8579747: neonatal mouse organ of Corti, WT, ATAC-seq; Mus musculus; ATAC-seq
OCs from WT and Bmi1-/- neonatal mice were dissected separately. Nuclei isolation was performed using lysis buffer containing 10 mM Tris-HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl2, and 0.1% IGEPAL CA-630. The ATAC-seq library preparation was conducted using the Hyperactive ATAC-Seq Library Prep Kit for Illumina (Vazyme, TD711) following the manufacturer's protocol.