PacBio HiFi sequencing of Meloidogyne javanica LongYF2-1 Whole genome amplification DNA
To investigate the false-positive rate of 6mA methylation sites identified based on IPD signals in the PacBio data of root-knot nematodes, we performed whole-genome amplification (WGA) using the DNA of the corresponding species. The amplified DNA was then sequenced using PacBio, and the 6mA signals in the WGA samples were analyzed. These WGA samples served as negative controls to subtract false-positive signals from the experimental samples.