GSM9105078: male liver, LKO, 20-22month, biorep3; Mus musculus; RNA-Seq
Total RNA was isolated using the Qiagen Rneasy Plus Mini Kit following the manufacturer protocol. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.