UCE target enrichment of Gnamptogenys_cofan_EX3657 : adult worker
Samples were extracted using column-based extraction kits. DNA was sheared to 400-600 bp using a DNA sonicator. Sheared DNA was then sent to the University of Utah genomics core facility for library preparation, target enrichment, and Illumina sequencing. Ultraconserved elements were enriched using an ant-customized RNA bait set (Arbor Biosciences; ant-specific hym-v2) that includes 9,898 baits (120 mer) targeting 2,524 UCE loci shared across Hymenoptera and a set of legacy markers (Branstetter et al. 2017). Approximately three million paired-end reads of enriched library and two million pair-end reads of unenriched library (WGS) were generated using Illumina NovaSeq X platforms (2x150 bp). The enriched and unenriched reads were combined into single R1 and R2 read files prior to upload to the SRA.