16S rRNAseq of mus musculus: intestinal contents
16S rRNA sequencing was performed at the University of Chicago Duchossois Family Institute in the Microbiome457 Metagenomics Facility (DFI MMF). DNA extraction from intestinal contents was performed using the458 QiaAmpPowerFecal Pro DNA (Qiagen, cat. # 51804) and the V4-V5 region of 16S rRNA- genes PCR amplified using459 barcoded dual-index primers (520FWD- AYTGGGYDTAAAGNG and 909REV-CCGTCAATTYHTTTRAGT). Illumina460 compatible libraries were generated using the Qiagen QIASeq 1-step amplicon kit (Qiagen, cat. #180419 and sequencing461 performed on an Illumina MiSeq platform using a 2250 Paired-End reads, generating >10,000 reads per sample.