GSM9076936: JunB-KO_JunB_OVEREXPRESSED_vehicle_replicate_03; Mus musculus; RNA-Seq
RNA from HoxB8-derived neutrophils was extracted using RNAeasy micro kit (Quiagen ®). RNA quality was checked using capillary electrophoresis (Agilent). Samples were submitted for whole RNA next generation sequencing in the Genomics Unit of CNIC. 200 ng of total RNA was used to generate barcoded RNA sequencing libraries using the NEBNext Ultra RNA Library preparation kit (New England Biolabs). Libraries were sequenced with HiSeq2500 (Illumina) to generate 50-nucleotide single reads, with a minimum of eight million reads per sample. RNA integrity was assessed using Agilent Bioanalyzer 2100, with RNA integrity numbers (RIN) between 9.8 and 10.0. RNA samples were processed by Novogene (Munich, Germany) for library preparation and sequencing, and all samples passed the quality control criteria. Strand-specific libraries were generated based on Novogene´s standard protocol. Samples were sequenced on an Illumina platform to produce 150 bp pairwise reads (PE150) per sample.