GSM9741320: B73_5dpi_cut _mock_rep3; Zea mays; RNA-Seq
RNA was extracted using Trizol (Thermo Fisher Scientific) and an RNA clean and concentrator-5 kit (Zymo Research) (Fall et al. 2019). RNA quality was verified on a 1.0% agarose gel. A total of 75 RNA samples were submitted for library preparation and sequencing at Roy J. Carver Biotechnology Center at the University of Illinois. Ribosomal RNA was removed with the Ribozero Plant + bacterial kit (Illumina) The RNA-seq libraries were prepared with a TruSeq Stranded mRNA-seq Sample Prep kit (Illumina). The libraries were pooled, quantitated by qPCR, and sequenced on one S4 lane for 151 cycles from both ends of the fragments on an Illumina NovaSeq 6000 (150 bp paired-end reads). Fastq files were demultiplexed using the bcl2fastq v2.20 Conversion Software (Illumina).