RNA sequencing of canine oral melanoma and matched peripheral blood pre- and post-radiotherapy
RNA sequencing of canine oral melanoma and matched peripheral blood pre- and post-radiotherapy
Tumor tissue was collected by Tru-cut biopsy and preserved in RNAlater™ at three time points: before RT (Pre-RT), immediately after the final radiation fraction (Post-RT 0 m), and 4 months post-RT (Post-RT 4 m). Peripheral blood was drawn from the external jugular vein into Tempus™ Blood RNA Tubes at two time points: before RT (Pre-RT) and immediately after the final radiation fraction (Post-RT 0m). All samples were stored at −80 °C until processing. Animal procedures were approved by IACUC (GNU-240826-D0172). Total RNA was extracted from tumor (Pre-RT, Post-RT 0 m, Post-RT 4 m) and blood (Pre-RT, Post-RT 0 m) by the service provider according to internal standard operating procedures. Libraries were prepared using TruSeq Stranded Total RNA Library Prep Gold Kit (Illumina, San Diego, CA, USA) for tumor samples and TruSeq Stranded Total RNA Globin Kit (Illumina, San Diego, CA, USA) for blood samples.