A-2 (CRX1824840; CRA027377)
The extracted mRNA is enriched using mRNA Capture Beads. After purification with beads, the mRNA is fragmented using high temperatures. The fragmented mRNA is then used as a template to synthesize the first strand of cDNA in a reverse transcription enzyme mixture system. While synthesizing the second strand of cDNA, end repair and A-tailing are completed. Next, adapters are ligated, and Hieff NGS DNA Selection Beads are used for purification to select target fragments. submitted_instrument_model: Illumina NovaSeq 5000