Coxiella burnetii- Mongolian rodents
Full-length bacterial 16S rRNA gene (V1V9) was amplified using universal primers (27F/1492R equivalents) and sequenced using Oxford Nanopore Technologies PromethION platform. PCR products from Coxiella-positive samples were barcoded using native barcoding kits, pooled, and sequenced on R10.4.1 flow cells. Basecalled FASTQ reads were used for taxonomic classification and SNP-based strain-level analysis of Coxiella burnetii.