Expanded Hyb-Seq phylogenomics of Burmeistera bellflowers: 2016_241
DNA was extracted from leaf tissues using the 2 CTAB method (Doyle and Doyle 1987), and Hyb-Seq libraries were prepared and sequenced by RAPiD Genomics (Gainesville, FL) on an Illumina HiSeq 3000 sequencer (Illumina, Inc., San Diego, CA) as described in Bagley et al. (2020). FASTQ files are provided for cleaned reads remaining after trimming the original FASTQ data files using Trimmomatic (Bolger et al. 2014; as TrimmomaticPE, with arguments '-phred33 -threads 1 <FASTQ file paths> ILLUMINACLIP:<path to FASTA file containing adapters etc.>:5:20:5 SLIDINGWINDOW:4:15 LEADING:20 TRAILING:20 CROP:250 HEADCROP:10 MINLEN:40') because in several cases raw data were not available.