GSM9168511: DNV_205_7; Rattus norvegicus; RNA-Seq
Total RNA was extracted from the diaphragm using TRIzol (Life Technologies, Carlsbad, CA). RNA integrity was analyzed using Bioanalyzer 2100 (Agilent, Santa Clara, CA, USA). Approximately ~1 ug of sample total RNA was used for cDNA library preparation performed according to the Illumina mRNA stranded poly-A tail enrichment Kit manufacturer's instructions (Illumina, San Diego, CA). RNA sequencing was carried out using the Illumina NextSeq 2000 (NextSeq 2x100pb, 8.4-13.9 million paired-end reads depth).