GSM9896094: HEK293 Minocycline polysome profiling Light polysome fraction replicate 3; Homo sapiens; RNA-Seq
Cytoplasmic lysates were separated by sucrose-gradient polysome profiling into monosome, light-polysome, and heavy-polysome fractions. ERCC spike-ins were added after fractionation and before RNA extraction from each fraction. RNA from each polysome fraction was converted into Illumina sequencing libraries and sequenced as single-end 75 nt reads on an Illumina NextSeq 500 instrument.