GSM9790508: mNPC LS, MOF-dTAG, negative control, H4K8ac, rep2; Mus musculus; OTHER
DNA fragments were isolated using the ChIP DNA Clean & Concentrator kit (Zymo Research) and eluted with 15 l elution buffer (provided by the kit). The library was prepared using 13 l DNA solution, mixed with 1 l of 10 M barcoded i5 and i7 primers separately, and 15 l NEBNext HiFi 2x PCR Master Mix. The PCR program included 72 °C for 5 minutes, 98 °C for 30 seconds, followed by 13 to 16 cycles of 98 °C for 10 seconds and 72 °C for 10 seconds, ending with 72 °C for 1 minute and holding at 8 °C. Ampure beads (39 l) were used for DNA purification. CUT&Tag