GSM8465472: VEH-SK-UT-1 16 RNA; Homo sapiens; RNA-Seq
We performed whole transcriptome analysis to determine the mechanism  underlying the inhibitory effect of BET protein inhibition on the uLMS. The SK-UT-1 cells  were treated with BET protein inhibitors JQ1 and I-BET 762 at a concentration of 5 µM  for 48 h. RNA was isolated using Trizol. RNA quality and quantity were assessed using  the Agilent bio-analyzer. Strand-specific RNA-Seq libraries were prepared using a  TruSeq total RNA-Seq library protocol (Illumina provided). Library quality and quantity  were assessed using the Agilent bio-analyzer, and libraries were sequenced using an  Illumina NovaSEQ6000 (illumine provided reagents and protocols).