GSM8515161: P3_UNTR-D; Homo sapiens; RNA-Seq
RNA was harvested at 72 hrs of activation of T-cells (Untreated and 100pM RTA-408 treated). Total RNA was extracted using RNeasy Plus Micro kit (Qiagen). 1.5ug of RNA was used for construction of sequencing libraries. 100ng of the RNA was used to perform Agilent TapeStation QC analysis to verify RNA integrity and generate RNA Integrity number (RIN). Using Nova-Seq 6000 S1 200 cycle reagent kit, 25 million pair-ended reads were recorded for construction of mRNA libraries.