RNA-Seq of sparus aurata:69 dph juveniles
Total RNA was extracted from whole juvenile gilthead seabream (Sparus aurata) using TRIzol reagent (Thermo Fisher Scientific, Waltham, USA). Twelve individualsfour biological replicates per vgll3 SNP genotype (AA, AG, GG)were selected based on prior genotyping and vgll3 expression profiling via qPCR. RNA integrity and purity were assessed by electrophoresis on 1.5% agarose gels and spectrophotometric analysis using a Nanodrop 2000 (Thermo Fisher Scientific, Waltham, USA). High-quality RNA samples were outsourced to Novogene (UK, Europe) for stranded mRNA library preparation using poly-A selection. Libraries were sequenced on the Illumina NovaSeq platform to generate 150 bp paired-end reads.