Genomic Investigation of a Chikungunya Outbreak in Cambodia, 20202022
Samples were processed as follows: serum was mixed 1:1 with 2X DNA/RNA Shield (Zymo Research) and pathogen RNA was extracted using Quick-RNA MicroPrep kit (Zymo Research). mNGS libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit (New England BioLabs)