Sequencing of Toxoplasma gondii isolated from cat brains
Qualified DNA samples were randomly sheared into 350-bp fragments using a Covaris cell disruptor. Library preparation was performed using the TruSeq Library Construction Kit, strictly adhering to the recommended reagents and consumables specified in the manual. The entire library preparation process included end repair, polyA tailing, sequencing adapter ligation, purification, and PCR amplification. The constructed libraries were sequenced using Illumina technology.