GSM9743604: Multiome, iPSC-derived otic progenitors, control (CA-26), ATAC; Homo sapiens; ATAC-seq
On the day of collection, iPSC-derived otic progenitor cells were dissociated into a single-cell suspension using Accutase. Cells were then subjected to negative selection for TRA-1-81 (pluripotency marker) followed by positive selection for CD271/p75 (early otic progenitor marker) using CD271 magnetic beads (Miltenyi Biotec). The CD271-enriched population was used for single-nuclei isolation following the 10X Genomics Nuclei Isolation for Single Cell Multiome ATAC + Gene Expression Sequencing demonstrated protocol (CG000365). Joint snRNA-seq and scATAC-seq libraries were prepared from the same nuclei using the Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Kit (10X Genomics, PN-1000283) following the manufacturer's user guide (CG000338). Transposed nuclei were partitioned into Gel Beads-in-emulsion (GEMs) on the Chromium Controller (Chromium-X). Gene expression (GEX) and ATAC libraries were prepared in parallel and sequenced at the Center for Genome Technology DNA Sequencing Core, John P. Hussman Institute for Human Genomics, University of Miami, on an Illumina NovaSeq 6000.