GSM9059527: 12month, replicate 3, scRNAseq; Homo sapiens; RNA-Seq
CD3+ T cells were FACS-purified from blood obtained from SCID-X1 patients at 14-weeks and 12-months post gene therapy.  Purified CD3+ T  cells were washed with PBS and 0.04% BSA before loading into a Chromium Next GEM Chip G (10X Genomics). GEM generation, cDNA amplification and library construction were performed following the protocol of Chromium Next GEM Single Cell 5′ Reagent Kits v2 (Dual Index, 10X Genomics) with target recovery of 5000-10000 cells per library/sample. The final 4 libraries were sequenced on the Illumina NovaSeq platform using 100bp paired-end with target reads of 500 million per sample. GEM generation, cDNA amplification and library construction were performed following the protocol of Chromium Next GEM Single Cell 5′ Reagent Kits v2 (Dual Index, 10X Genomics) with target recovery of 5000-10000 cells per library/sample