18S rRNA gene V4 amplicon sequencing of seawater at station G4, 64 m
The V4 region of the 18S rRNA gene was amplified using forward primer 5'-CCAGCASCYGCGGTAATTCC-3' and reverse primer 5'-ACTTTCGTTCTTGATYRA-3'. Three technical PCR replicates were pooled and subjected to paired-end Illumina sequencing.