GSM8898223: young_sirt6_tg_10; Mus musculus; RNA-Seq
Trizol extraction (Phenol/chlorophorm) RNA-seq libraries were prepared at the Crown Genomics Institute of the Nancy and Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science using the INCPM-mRNA-seq. Briefly, the polyA fraction (mRNA) was purified from 500 ng of total input RNA, followed by fragmentation and the generation of double-stranded cDNA. After Agencourt Ampure XP beads cleanup (Beckman Coulter), end repair, A base addition, adapter ligation, and PCR amplification were performed. Libraries were quantified by Qubit (Thermo Fisher Scientific) and TapeStation (Agilent)