GSM9468297: hTECs treated with Ad-null replicate 2; Homo sapiens; RNA-Seq
Sample QC of input Total RNA samples assessed by measuring the RNA Integrity Number (RIN) and concentration using a Agilent 2100 instrument. RNA purity and contamination can be assessed through UV-spectrophotometry using a Nanodrop spectrophotometer. A total amount of 2 μg RNA was used as input material for the RNA sample preparation. Use the NEBNext®Single Cell/Low Input cDNA Synthesis & Amplification Module and PacBio Iso-Seq Express Oligo Kit to perform first-strand cDNA synthesis (reverse transcription and template switching) and PCR amplification. SMRTbellTM Template Prep Kit was used to generate SMRTbellTM libraries by following to the processes of DNA Damage repair, end-repair, A-tailing and adaptor ligation. library quality was assessed on the Agilent Bioanalyzer 2100 system. At last，Binding the primers and enzyme to the SMRT template to form a complete SMRT bell library.