Fumigated soil day 0 site 2
The archaeal 16S rRNA genes were amplified with previously reported primers 25F and A571R and containing the 454 FLX adapters, applying 25 cycles. The product of five reactions was pooled and constituted a single library. The pyrosequencing libraries were purified and pooled in equimolar amounts. The DNA was sequenced by Macrogen Inc. with a Roche 454 GS-FLX Titanium System pyrosequencer.