Ghana Monkeypox virus hybrid-capture genome sequencing and assembly
Monkeypox virus genomic DNA was extracted and enriched using Twist Comprehensive Viral Research panel (Twist Bioscience, USA) following the Illumina DNA Prep with Enrichment protocol. Illumina sequencing libraries were prepared with IDT unique dual indexes and pooled using a 1-plex enrichment strategy before sequencing on an Illumina MiSeq (v3 reagent kit). Oxford Nanopore Technologies (ONT) libraries were prepared using a hybrid-capture workflow also utilizing the Twist Comprehensive Viral Research panel (PathoSeq Bio, LLC) with cDNA synthesis, tagmentation, target enrichment, and ONT-specific barcoding. Libraries were sequenced on an ONT MinION using R10 chemistry.