Whole genome sequencing of single neuron PTA amplified DNA from homo sapien cerebellum
Single neuronal nuclei were isolated using FANS with staining of NeuN antibody from frozen brain (cerebellum). The nuclie were lysed and the genome amplifeid using primary template-directed amplification (PTA). Libraries were prepared using Kapa HyperPlus modified for PTA and sequenced to a target depth of 22X on an Illumina NovaSeq X Plus.