GSM8577733: Treat-1; Mus musculus; ATAC-seq
Nucleus extraction: For cell samples, refer to the method in the document "ATAC-seq: A Method for Assaying Chromatin Accessibility Genome-Wide" to lyse cells and obtain nuclei. For frozen tissue samples, refer to the method in the document "An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues" to extract nuclei. Transposition reaction: Take 40,000 nuclei and perform a transposition reaction with Tn5 enzyme (Illumina). The transposed DNA fragments are recovered using the MinElute PCR Purification Kit (Qiagen). Use 1X NEBNext High-Fidelity PCR Master Mix (New England Biolabs, MA) for PCR amplification. The amplified products are recovered using the MinElute PCR Purification Kit (Qiagen).