GSM9063717: lib_nonamer_2; Mus musculus; OTHER
Genomic DNA was extracted from 5 × 10? cells using the Qiagen DNeasy Blood & Tissue Kit, according to the manufacturer's instructions. Pre- and post-recombination 12?23 RSS fragments were amplified by two rounds of PCR. Sequencing libraries were prepared for Illumina next-generation sequencing and sequenced on the Illumina MiSeq platform using the MiSeq Reagent Kit v2 (Illumina), following the manufacturer's protocol.