TK13 (CRX1831232; CRA027612)
DNA was extracted using the cetyltrimethylammonium bromide (CTAB) metho. Genomic DNA is randomly broken into short DNA fragments by enzymes and then subjected to flat end repair. Then connect dA tails at both ends of the DNA fragment and connect sequencing adapters. The DNA fragments with added adapters were purified using AMPure XP magnetic beads, and fragments in the range of 300-400bp were selected for PCR amplification. The constructed library undergoes purification, library testing, and Hiseq X10 PE150 sequencing on the computer. submitted_instrument_model: Illumina HiSeq X Ten