Bulk RNA Seq of mouse aged 3-months-old
Following RNA extraction as described above, RNA was dried in stabilisation tubes overnight (NovogeneAIT Genomics Singapore Pte, Singapore) and shipped to NovogeneAIT Genomics Singapore for bulk RNA sequencing. Sequencing libraries were constructed with the Illumina TruSeq unstranded RNA library preparation kit using polyA selection for mRNA enrichment, then sequenced on the Illumina NovaSeq6000 platform acquiring~20 million, 150 base-pair, paired-end reads per sample.