GSM9316376: EV 3D 3; Homo sapiens; RNA-Seq
RNA was isolated using TRI Reagent (Invitrogen). RNA was frozen at -80C and shipped on dry ice to Novogene for processing. Messenger RNA was purified from total RNA using poly-T oligo-attachedmagnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers. Then the second strand cDNA was synthesized using dUTP, instead of dTTP. The directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection.