GSM8606171: DS A455D PMPs transplanted, DSAD-tau treated, 10 mo, rep 1; Homo sapiens; RNA-Seq
Chimeric mice were anesthetized and perfused with cold DPBS (1x). Brains were dissected and dissociated using Adult Brain Dissociation Kit (Miltenyi) according to the manufacturer's instructions. Human cells were isolated by mouse cell depletion kit (Miltenyi) following the manufacturer's protocols. Isolated cell suspension then loaded to the Chromium controller for mRNA barcoding and reverse transcription using Chromium Next GEM Single Cell 3' Kit v3.1. scRNA-seq libraries were constructed using Chromium Next GEM Single Cell 3' Kit v3.1 and Dual Index Kit TT Set A, following the manufacturer's protocols. Quality control of the scRNA-seq libraries were performed by Agilent 2100 Bioanalyzer, and libraries were sequenced in Novaseq 6000 S4 flow cells.