GSM9896677: CAF40_DMSO_4h_r3; Saccharomyces cerevisiae; RNA-Seq
24 OD units were collected by centrifugation, and snap frozen in liquid nitrogen. Per 10 OD units, RNA was extracted with Tris-EDTA-SDS (TES) buffer (10 mM Tris-HCl pH 7.5, 10 mM EDTA, 0.5% SDS) and Acid Phenol:Chloroform:Isoamyl alcohol (125:24:1, Ambion) at 65°C for 45 minutes. Precipitation was carried out. rDNase (Machery-Nagel) treatment was carried out, before spin column purification (Machery-Nagel). For degron strains, libraries from 100 ng of RNA were generated using the NEBNext Ultra II Directional PolyA mRNA Kit (modules K0105 and K0078), following the manufacturer's protocols. Paired-end sequencing (100 bp) was performed on an Illumina NovaSeq X platform, with a minimum depth of 20 million reads per sample.