Aerolito saline cave sediment, station 3, trip 4, rep 1
Environmental DNA was extracted from bulk sediment collected from submerged cave systems using the DNeasy PowerMax Soil Kit. Triplicate PCR amplifications using general eukaryotic primers and targeting the 18S rRNA gene were performed per sample, and replicate PCR products were pooled and pruified using AMPure XP beads prior to Illumina sequencing.