GSM9468373: HFF treated with Oleic Acid, Rep 3; Homo sapiens; RNA-Seq
Total RNA was extracted from the cells using TRIzol reagent (Invitrogen) following the manufacturer's instructions. RNA integrity and concentration were assessed using the Agilent 2100 Bioanalyzer. Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations. Index codes were added to attribute sequences to each sample. The libraries were sequenced on an Illumina NovaSeq 6000 platform to generate 150 bp paired-end reads.