GSM9312729: B lymhocyte, C9-ALS, line1, G4C2 probe; Homo sapiens; OTHER
Cells were fixed with 1% glutaraldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a sonicator (Diagenode) until the chromatin DNA was 100–500 bp in size. ChIRP-seq libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit complemented with  NEBNext Multiplex Oligos for Illumina by following the manufacturer's manual. Libraries were validated using the Agilent High Sensitivity DNA Kit.