GSM9026197: HB3; Mus musculus; RNA-Seq
Total RNA was isolated using the Trizol Reagent (Invitrogen Life Technologies), after which the concentration, quality and integrity were determined using a NanoDrop  spectrophotometer(Thermo Scientific).3 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using NEBNext Ultra II RNA Library Prep Kit for Illumina(New England Biolabs Inc;Ipswich,Massachusetts,USA).