RNA-Seq of soilHse
Extract high-quality DNA, and use Nanodrop to assess DNA purity, concentration, and integrity. Use agarose gel electrophoresis to analyze the fragment size distribution.Amplify the full-length 16S rRNA gene using specific primers, and precisely quantify the amplification products using Qubit. Analyze the fragment size distribution of the amplification products via agarose gel electrophoresis.Perform end repair and dA-tailing on the amplification products.Ligate sequencing adapters to the amplification products.Purify the library using magnetic beads.Load the library onto an R10.4 Flow Cell and perform sequencing using a PromethION P48 sequencer (Oxford Nanopore Technologies, Oxford, UK)