VDJ Seq of Mus Musculus: Sorted CD45+CD11b-CD3+ cells of Liver of mice bearing cutaneous melanoma
Tumor and liver tissues were excised, mechanically dissociated, and filtered to obtain single-cell suspensions. Tumors were enzymatically digested with Liberase DL; liver leukocytes were enriched using a Percoll gradient. Cells were stained with TotalSeqC antibodies for hashing and with CD45-BV510, CD3-PerCP-Cy5.5, CD11b-PE, and eFluor 780 for FACS. CD45 CD11b CD3 cells were sorted into BSA-coated tubes using a BD FACSAria Fusion with a 100m nozzle.