Environmental DNA metabarcoding of Macao wetland (COI)
Environmental DNA extracted from water samples collected across urban wetlands in Macao was amplified targeting a ~313 bp fragment of the mitochondrial cytochrome c oxidase subunit I (COI) gene using mlCOIintF and HCO2198 primers. Amplicons were purified, pooled, and subjected to library preparation and quality control by BGI Genomics. Sequencing was performed on the DNBSEQ-G400 platform with paired-end 300 bp reads.