GSM9263478: SPF, Lung, immune cells+non-immune cells, no-irradiation; Mus musculus; RNA-Seq
Lung tissues were harvested from mice and processed into single-cell suspensions using the Mouse Lung Dissociation Kit (Miltenyi Biotec, Cat# 130-095-927) according to the manufacturer's protocol. Lung tissues were minced and enzymatically digested using the enzyme mix provided in the kit, with mechanical dissociation performed on the gentleMACS Octo Dissociator (Miltenyi Biotec). The resulting cell suspensions were filtered through a 70-µm cell strainer (Fisherbrand, Cat# 22363548) and treated with ACK lysis buffer (Invitrogen, Cat# 00-4333-57) to remove red blood cells. Libraries were sequenced on the NovaSeq X Series platform using the 10B reagent kit. NovaSeq Control Software v1.2.2.48004 was used for instrument operation, and Illumina BCL Convert v4.2.7 was used to convert base call (BCL) files into FASTQ format. Sequencing targeted approximately 10,000 cells per sample.