GSM9321910: Neurons replicate 2 derived from iPSC replicate 3; Homo sapiens; RNA-Seq
RNA was extracted using TRIzol™ Reagent according to the manufacturer's instructions Sequencing libraries were prepared using the stranded rRNA-depletion workflow (Ribo-Zero Plus rRNA Kit; Illumina), which removes bacterial and mammalian rRNA as well as human globin transcripts. Sequencing was performed on an Illumina NovaSeq 6000 platform using an S4 flowcell to generate paired-end 150 bp reads, with a target depth of approximately 100 million reads per sample.