GSM9060305: Mouse motor cortex, Q331K, 10mth, rep2; Mus musculus; RNA-Seq
Animals were perfused with ice-cold PBS on ice. The MC was dissected on ice and flash-frozen in 100% ethanol and dry ice. The tissue samples were stored at -80 degrees for a few days. Samples were shipped to Azenta, and total RNA were extracted according to Azenta's sample preparation protocols Strand-specific library preparation with rRNA deplettion was performed according to Azenta's library preparation protocol to be compatible with Illumina sequencing