Amplicon barcoding sequencing for full-length 16S: SP78 (Oxford Nanopore)
Full-length bacterial 16S rRNA gene PCR amplicons (27F-1492R) prepared using the Microbial Amplicon Barcoding Kit 24 V14 (SQK-MAB114.24). Barcoded amplicons pooled and sequenced on ONT MinION flow cell.