GSM8898187: Isolated DCs from D28 hTOs - Replicate 1; Homo sapiens; RNA-Seq
Cells were isolated from hTOs seeded with primary early thymic progenitors (ETPs) at the late phase of maturation (D28) by FACS sorting with CD45-EPCAM+HLA-DRhigh phenotype (TEChigh), CD45-EPCAM+HLA-DRlow (TEClow) and CD45+EPCAMlo (DC) Libraries were prepared with the SMART-Seq v4 Ultra Low Input RNA kit and Illumina Nextera XT DNA Library Preparation Kit following manufacturer's protocols.