GSM8606204: control littermate P0 rep2; Mus musculus; RNA-Seq
P0 neonatal mouse hearts were homogenized in TRIzol (Invitrogen) for RNA extraction according to the manufacturer's instructions. The concentration and quality of purified RNA was assessed by Agilent Bioanalyzer 2100. cDNA libraries were constructed using an Illumina TruSeq stranded mRNA kit according to manufacturer's instructions. Libraries were sequenced with an Illumina NovaSeq 6000 sequencer to a sequencing depth of 80-100 million reads per sample.