GSM9315016: COTC021-0722-T; Canis lupus familiaris; Bisulfite-Seq
DNA was isolated from tumor and normal tissues preserved in RNAlater using Qiagen Allprep DNA/RNA Mini Kit (Cat#80204). Qubit Fluorometer 2.0 with the Qubit dsDNA BR assay (ThermoFisher Scientific) and the TapeStation genomic DNA assay (Agilent Technologies) was used to assess the DNA quantity and quality. Samples were sequenced on 8 NovaSeq 6000 S4 runs using EZ DNA Methylation-Gold Kit/Accel NGS Methyl-Seq DNA library preparation and paired-end sequencing.