AMPLICON of Severe acute respiratory syndrome coronavirus 2: Human 9063
The 9063 library was prepared using the Molecular Loop Viral RNA Target Capture Kits (Molecular Loop) in accordance with manufacturer's recommendations. Briefly, 6uL of RNA was reverse transcribed and capture probes annealed in a 16 hour incubation at 55ºC. The probes were then enzymatically circularized to capture the viral genome and add Unique Molecular Indexes followed by amplification of circularized cDNA targets using 27 cycles of PCR. Libraries were pooled by volume and cleaned-up by magnetic bead purification. Final libraries were quantified using fluorescent-based assays including PicoGreen (Life Technologies) or Qubit Fluorometer (Invitrogen), and Fragment Analyzer (Advanced Analytics) and sequenced on Illumina NovaSeq 6000 with 2x150bp reads targeting N/A read coverage.