GSM9063337: auricular chrondroytes, cultured in HATG-Alg bioink, 3 weeks, rep1; Homo sapiens; RNA-Seq
HATG and HATG-Alg constructs were snap frozen in liquid nitrogen and subjected to cryopulverization. The powder was collected in 1.4 mL TRIzol Reagent (Invitrogen, 15596026), and RNA was isolated according to the manufacturer's specifications. RNA concentration was determined using the Qubit RNA High Sensitivity (HS) Kit (Invitrogen, Q32852). RNA quality and integrity were assessed using a 2100 Bioanalyzer (Agilent Technologies) with the RNA 6000 Pico Kit (Agilent, 5067-1514), and only samples with an RNA Integrity Number (RIN) > 9 were used for library preparation. RNA-seq libraries were prepared using the TruSeq Stranded Total RNA Library Prep Kit (Illumina, RS-122-2202) with 100 ng of total RNA per sample. Libraries were uniquely dual-indexed using the TruSeq UDI system (Illumina) and sequenced on a NovaSeq 6000 with 50 bp paired-end to a depth of ~ 20 million fragments per sample. Illumina TruSeq Stranded Total RNA Library Prep Kit (Cat. No. RS-122-2202)