Whole-genome long-read sequencing (PacBio HiFi) of Marmaronetta angustirostris: blood
High-molecular-weight genomic DNA was quality-checked and sheared to the desired fragment size. SMRTbell libraries were prepared using standard PacBio protocols, including DNA damage repair and end repair, followed by ligation of hairpin adapters to generate closed circular DNA molecules. Unligated fragments were removed using exonuclease treatment. Libraries were size-selected and purified using AMPure PB beads. Sequencing primers were annealed to the SMRTbell templates, and DNA polymerase was bound prior to sequencing.