GSM9141119: Pv neurons nuclei. PTU mice 6; Mus musculus; RNA-Seq
Nuclei were isolated from individual cortex frozen in liquid nitrogen and nuclei were isolated with a cell sorter. RNA were extracted from sorted nuclei (RNeasy Micro Kit, Qiagen ref 74004) and quantified using Tapestation4150 (Agilent). cDNA preparation: Smaartseq v4 Takara (ultralow input); cDNA libraries preparation: NexteraXT. Run on Mid output flowcell Nextseq500 Illumina 1x75bp