Ion Torrent PGM sequencing of SAMD01612104
The 16S rRNA gene was amplified using the following primers: 8F (5'-AGA GTT TGA TYM TGG CTC AG-3') with Ion Torrent adaptor A and sample-specific 8-base tag sequence and 338R (5'-TGC TGC CTC CCG TAG GAG T-3') with the Ion Torrent trP1 adaptor sequence. PCR amplification was carried out using KOD DNA polymerase. Reads were excluded from the analysis if they were shorter than 200 bases, had an average quality score less than or equal to 25, or did not include the correct forward and reverse primer sequences.