GSM9709993: Differential expression of microRNAs in early-stage endometriosis in baboons, Sample 72; Papio anubis; RNA-Seq
RNAs were extracted using the MagMAX™ total RNA Isolation Kit. Total RNA quality was determined by estimating the A260/A280 and A260/A230 ratios by nanodrop. RNA integrity was determined by running Agilent Fragment Analyzer, which measures  the ratio of the ribosomal peaks.  Samples with RIN values of 7 or greater are used for library prep. RNA was normalized to a starting input between 1ng-500ng. Adapters were ligated sequentially to the 3' and 5' ends of miRNAs prior to cDNA synthesis with UMI assignment, cDNA cleanup, amplification, and final library cleanup The library was run on the Agilent Tapestation and size selection was performed if there was excess adapter dimer. The library was then quantified by qRT-PCR using a commercially available kit (KAPA Biosystems). Sample concentrations were normalized to 2 nM and loaded onto an Illumina NovaSeq X flow cell at a concentration that yields 10-25 million passing filter clusters per sample.