GSM9078934: L1_B_CTRL; Rattus norvegicus; OTHER
Tissue samples were collected from both EG and CT rats at five (D5), ten (D10), twenty (D20), and forty (D40) days post-SE. The rats were anesthetised with Euthasol prior to decapitation. Brains were then dissected and separated into two hemispheres. Immediately after dissection, the hemispheres were frozen in isopentane cooled on dry ice and stored at -80°C. The right hemisphere was used for spatial transcriptomic analysis. It was embedded in Optimal Cutting Temperature (OCT) medium and cryosectioned coronally. Sections were cut until the hippocampus was fully visible. The tissue was then trimmed with a razor blade until it fit into a 6 mm wide square, with the hippocampus at its centre. A 10 µm tissue section was placed on one capture area of a Visium Gene Expression Slide (10x Genomics), while 6 sections before and 6 after were placed on regular glass slides (3 sections per slide). The Visium slide was stored at -80°C until further processing, and the regular glass slides were stored at -20°C. Each Visium Gene Expression slide consists of 4 capture areas, Spatial transcriptome sequencing libraries were prepared with 10xGenomics Visium Spatial Gene Expression Slides v1 & Reagent kit according to manufacturer's construction