GSM8464226: C2C12_myotubes_ATACseq_Ctrl_N2; Mus musculus; ATAC-seq
C2C12 cells were seeded in triplicate in 12-well plates 7 days before the experiments. After reaching confluence, myoblasts were induced by culturing in DMEM containing 2% horse serum for 4-7 days. Differentiated C2C12 myotubes were induced by incubation with C26 conditioned medium for 24 hours to establish the muscle cell degradation model. The cells were washed with PBS, resuspended in lysis buffer, and the nuclei were spun down. The nuclei were then transferred to the transposition reaction buffer and incubated at 37°C for 30 minutes with 105 cells. Genomic DNA was extracted using the Qiagen MinElute PCR Purification Kit. The ATAC-seq library was prepared according to a previously reported protocol (Buenrostro et al., 2015). The purified ATAC-seq DNA library mix was sequenced on an Illumina NovaSeq 6000 (PE150 reads). The ATAC-seq Library preparation was performed through the manufacturer's introduction.