Sample16
, total RNA from exosomes was isolated following the manufactures protocol of miRNeasy Mini kit (Qiagen). Isolated RNA was run on Agilent 2100 BioAnalyzer to determine the amount of miRNA from the total small RNA concentration. The amount of RNA used in library preparation was standardized to 10 ng of miRNA for each sample. Small RNA sequencing libraries were synthesized and gel size-selected using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB).