DH22 (CRX2096070)
The nucleic acid of the samples was extracted, and the extracted nucleic acid samples were quantified using Qubit and analyzed by 1% agarose gel electrophoresis to determine that the extracted genomes were complete and consistent with the expected genome size. The qualified nucleic acid samples were used for the construction of DNA or RNA second-generation sequencing libraries, and NGS libraries with an insertion fragment range of 200-500bp were obtained. After the libraries passed quality control, PE150 sequencing was performed using the Illumina Novaseq 6000 platform, and the Raw data obtained from the sequencing will be used for subsequent bioinformatics analysis. The clean reads were de novo assembled using the metaSPAdes software, and different k-mer lengths were selected for testing to obtain the best assembly result. Then, bwa was used to calculate the coverage of the genome sequence.