sRNA-Seq of Vibrio alginolyticus: Bacterial extracellular vesicles
A total amount of 1g total RNA per sample was used as input material for the small RNA library. Sequencing libraries were generated using QIAseq miRNA Library Kit (Qiagen) following manufacturers recommendations. The activated 5 and 3 adaptors were ligated to the total RNA, respectively. Then the adaptor-ligated RNA was transcribed into first-strand cDNA by using reverse transcriptase and random primer. A PCR reaction was performed using primers complementary for 11-12 cycles, fragments of appropriate size were isolated by a 6% Novex TBE PAGE gel. After quantified by Qubit 4.0.