GSM8724542: piggybac-SA-1; Homo sapiens; RNA-Seq
RNA was extracted using standard Trizol RNA extraction kit. RNA quality was determined by examining A260/A280 with NanodropTM NanoDrop (Thermo Fisher Scientific Inc). RNA integrity was confirmed by agarose gel electrophoresis. Qualified RNAs were then quantified by Qubit3.0 with QubitTM RNA Broad Range Assay Kit (Life Technologies，Q10210). 2 μg total RNA was used for library preparation using KCTM Stranded mRNA Library Prep Kit for Illumina (Wuhan Seqhealth, DR08402) following the manufacturer's instruction. PCR products at 200-500 bps were enriched, quantified and sequenced on DNBSEQ-T7 sequencer (MGI Tech Co., Ltd. China).