GSM9108837: CD8+ T Cells, DiSBey, Acute Stimulation, Day 6, chr1, batch1, biol_rep 3; Mus musculus; RNA-Seq
Simultaneous genomic DNA and RNA of murine CD8+ T cell samples (Figure 2A) were isolated using Quick-DNA/RNA MagBead (Zymo R2130). For esTAG-seq, genomic DNA was tagmented with UMI-loaded Tn5 transposase (seqWell 301230). For insertion site enrichment with PCR, primers targeting SB inverted regions were paired with the p5 forward primer in sequential nested PCR reactions. PCR products were purified with SPRI Beads and sequenced with Element Aviti 2x150 bp with a 20 nt i5 index read (to sequence the Tagify 10 nt i5 index and 10 nt UMI sequences)