GSM9241504: Bone Marrow Derived Macrophages, LL2 WT Conditioned Media, 100nM CDDO-ME, Replicate 2; Mus musculus; RNA-Seq
Total RNA was extracted from macrophages using RNeasy mini kit (Qiagen). Library construction was completed by Novogene as follows: Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification