16S Amplicon Sequencing of Soil Microbiome:Sediment samples subjected to high thallium impact loading,day 7,sample 1
DNA library fragment integrity and insert size length were measured using Qsep400. After the insert size meets the expectation, the on-machine sequencing will be carried out. After the library detection is qualified, different libraries will be carried out to the "owcell" according to the requirements of effective concentration and target on-machine data volume. The cBOT clusters were sequenced using the PE250 mode of Illumina's Novaseq 6024 high-throughput sequencing platform