GSM8515925: N2a, SaKKH-ABE, sgRNA4, replicate2 [amplicon]; Mus musculus; OTHER
Genomic DNA was extracted from tissues using TIANamp Genomic DNA Kit (DP304, Tiangen, China) The sgRNA-targeted loci were amplified using Taq PCR MasterMix (KT211, Tiangen, China) and purified by TIANgel Purification Kit (DP219, TIANGEN).