Whole-genome DNA sequencing of Enterococcus mundtii from soil
DNA was isolated from each of the samples using a Qiagen blood and tissue kit, following the protocol for extracting DNA of Gram positive organisms. DNA sample purity was determined through NanoDrop, and a Qubit fluorometer was used to determine the DNA concentration of each sample. Library preparation and whole-genome sequencing were performed at SeqCoast.  Libraries were prepared with the Illumina DNA Prep tagmentation kit and IDT For Illumina Unique Dual Indexes. An Illumina NextSeq2000 (300 cycle flow cell, 2x150bp paired-end reads) was used for sequencing. DRAGEN (v3.10.12) was used for demultiplexing and trimming.