GSM8606208: E13.5 dermal SmoM2YFP mutant; Mus musculus; RNA-Seq
Dorsolateral/flank skin was microdissected from E13.5 littermate control and mutant embryos and dissociated into a single-cell suspension using 0.25% trypsin (Gibco, Life Technologies) for 20 min at 37 °C. After genotyping, two to three embryos were pooled by condition. Single-cell suspensions were then stained with DAPI (Thermo Fisher Scientific, NBP2-31156) before fluorescence-activated cell sorting. Chromium Single Cell 3' GEM Library and Gel Bead Kit v3.1 (PN1000121) were used according to the manufacturer's instructions in the Chromium Single Cell 3' Reagents Kits V3.1 User Guide. After cDNA libraries were created, they were subjected to Novaseq 6000 (Illumina) sequencing. For each scRNA-seq experiment, control and littermate mutant samples were prepared in parallel at the same time, pooled and sequenced on the same lane.