RNA-seq of C. elegans, HT115 fed 20C
The mRNA sequencing libraries were prepared by the Bauer sequencing core (Harvard University) using 600ng of total Trizol-extracted RNA from the same worm samples prepared above. The KAPA mRNA HyperPrep kit was used for library construction. Small RNA and mRNA libraries were pooled and sequenced on the Illumina NextSeq 1000 platform using a single-end 100 base pair read length and a P2 flow cell.