RNA-Seq of Homo sapiens: gastric cancer cell line MGC-803R
Total RNA was extracted from docetaxel-resistant MGC-803R gastric cancer cells using TRIzol reagent. RNA quality and integrity were assessed using an Agilent 2100 Bioanalyzer. Sequencing libraries were prepared from high-quality RNA samples following standard Illumina RNA-seq library preparation protocols, including mRNA enrichment, fragmentation, cDNA synthesis, end repair, adapter ligation, and PCR amplification. Libraries that passed quality control were sequenced on an Illumina high-throughput sequencing platform to generate paired-end reads. Differential gene expression analysis was performed using DESeq2 and edgeR with thresholds of adjusted p-value < 0.05 and |log2 fold change| > 1.