GSM8726154: FMRP-cTag CLIP, CA1, Opto, 30 minutes, replicate 3; Mus musculus; RIP-Seq
After optogenetic activation and UV-crosslinking, tissue was dissected from acute hippocampal slices in HBSS with 0.1 mg/mL cycloheximide. Tissue was homogenized in lysis buffer (1x PBS, 0.1% SDS, 0.5% NP-40, 0.5% Sodium deoxycholate supplemented, 1X cOmplete Mini EDTA-free Protease Inhibitor (Roche) and 0.1 mg/mL cycloheximide) by mechanical homogenization. FMRP-cTag-CLIP was performed as described previously (see associated paper for details). RNA tags were isolated and cloned using the BrdU-CLIP method as described in associated paper.