RNA-seq of Candida albicans SC5314: bulk
RNA was extracted and purified using the MasterPure Yeast RNA Extraction Kit (LGC Biosearch Technologies, Hoddesdon, Herts, UK) with minor adaptations to the base protocol; specifically, DNase I (Thermo Fisher Scientific) treatment of RNA was extended to 1 hr to ensure removal of all DNA prior to storage at -80C. RNA concentration and purity was measured via Nanodrop, and poly(A) RNA was extracted from 1ug of RNA for each sample with the NEBNext Poly(A) mRNA Isolation Module (NEB #E7490). The purified mRNA was used to construct cDNA libraries with the NEBNext Ultra II Directional RNA Library Prep Kit (NEB #E7760) dUTP synthesis method.