RNA-Seq of Arcopilus aureus
Total RNA was extracted from the mycelia of A. aureus grown for 10 days on PDA  using TRIzol reagent (Thermo Fisher Scientific) following the manufacturers protocol. PolyA selection was performed on the total RNA using oligo(dT) magnetic beads to enrich for mRNA. The enriched mRNA was then subjected to library construction using the VAHTS Universal V10 RNA-seq Library Prep Kit (Vazyme Biotech).