Targeted capture sequencing of Homo sapiens: CRISPR/Cas9-edited iPSC clone 149-1-20v6
Genomic DNA was fragmented and enriched by hybridization capture using probes flanking CRISPR/Cas9 target sites and the STAG2 duplication breakpoint. Paired-end 150 bp reads were generated to characterize on-target structural alterations and plasmid integrations.