GSM9743882: Engineered Heart Tissue, day 30, biol rep 2; Homo sapiens; RNA-Seq
Engineered heart tissues were pooled from 2x 48 well plates and incubated at 37° C for 1.5-2 hours in a dissociation solution containing 1mg/ml Collagenase II,10 mM HEPES pH 7.4, 2 mM Thiazovivin, and 30 mM N-Benzyl-p-Toluenesulfonamide to obtain a single cell suspension. cells were filtered through 70 um cell strainer. Libraries were constructed according to manufacturers specifications using the 10X Chromium platform and 3' V3 chemistry targeting 10,000 cells per library.