GSM9648655: preLSC, 0min, control, rep1; Mus musculus; OTHER
Total RNA was extracted using QIAzol and alkylated in the dark room following manufactures protocol from Lexogen catabolic kit (cat. no. 062.24). 500 ng of total RNA was used for library preparation and 16 PCR cycles were performed using Lexogen QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (cat. no. 015.24).