Mouse cecal metagenome FELIX_MS942 NovaSEQ reads
Metagenomic libraries were prepared from 0.5 g of total DNA from three replicated samples per terrace and sequenced using the NovaSeq platform. DNA was quantitated using a Qubit fluorometer and 0.5 g in 50ul was sheared using a Diagenode Bioruptor sonicator. Agarose gel electrophoresis was performed on an aliquot of sheared DNA to determine the size range (500bp optimum). For some samples, multiple rounds of shearing were necessary. Libraries were constructed from the sheared DNA using the NEBNext Ultra Library Prep Kit from NEB (catalog no. E7370L) for Illumina sequencing on both MiSeq and NovaSeq instruments. For Nanopore sequencing, 1 g of total DNA from each sample was processed using the ligation sequencing kit (SQK-LSK109) for long reads.