16S guano
Sequencing was performed at a commercial genomics facility (China) on the Illumina MiSeq system in SINGLE-end mode (2  150 bp). For bacterial community analysis, the V3V4 hypervariable region of the 16S rRNA gene was amplified using primers 341F/806R 58. For fungal communities, the ITS1ITS2 region was amplified using primers ITS1 (5-TCCGTAGGTGAACCTGCGG-3) and ITS2 (5-GCTGCGTTCTTCATCGATGC-3) 59,60. Both libraries were sequenced independently