GSM9317108: MCF7 Wildtype hypoxia rep3; Homo sapiens; RNA-Seq
RNA was extracted using Trizol and cleaned up using Qiagen RNeasy column. The polyA RNAseq libraries were prepared with Illumina's 'TruSeq Stranded mRNAseq Sample Prep kit' (Illumina), polyadenylated mRNAs were captured with oligodT beads. The total RNAseq were prepared with the Watchmaker Stranded mRNA library kit after Ribosomal RNAs were removed with the Fastselect HRM kit (Qiagen).