GSM9614314: BATF3 CD8+ T cell Day 14, Donor U2; Homo sapiens; ATAC-seq
ATAC-seq library preparation was performed using the Active Motif ATAC-seq kit (Cat#53150). Briefly, 50,000 cells were washed in cold PBS, lysed to isolate nuclei, and subjected to Tn5 transposase reaction for simultaneous DNA fragmentation and adapter insertion. Following Tn5 transposition, DNA was purified and PCR amplified using Illumina-compatible primers. Library quality was confirmed by Bioanalyzer. Libraries were sequenced on Illumina NovaSeq X Plus (paired-end 150bp) at Macrogen Japan.