GSM9468788: proximal_colon_mucosa,half_FV_diet,dss_treated,rep3; Sus scrofa; RNA-Seq
Whole proximal colon sections (~5 cm) were excised and the mucosa layer was  separated from the submucosal layer, stored in cryotubes, flash frozen in liquid nitrogen, and stored at −80°C until ready for RNA isolation. Total RNA was extracted using the TRI reagent (Zymo Research, Irvine, CA). Residual genomic DNA removed using TURBO DNA-free kit (Invitrogen, Carlsbad, CA) with RNAseOUT (Invitrogen, Carlsbad, CA). RNA quality and concentration was assessed by gel electrophoresis using Experion RNA Standard Sensitivity Analysis Kit (Bio-Rad, Hercules, CA). All samples had an RNA quality indicator (RQI) greater than 8.5 RNA sequencing libraries were prepared with Illumina TruSeq RNA Sample Prep v2 kits (Illumina, San Diego, USA). Libraries were sequenced on an Illumina Next Seq 500 (Illumina, San Diego, CA) and a high output flow-cell to generate 150 base-pair single-end reads.