GSM9107735: ATAC_Prim_B92_Vehicle_rep1; Mus musculus; ATAC-seq
For ATAC-seq, cells were lysed and incubated with transposition reaction mix containing PBS, 1% Digitonin, Tween-20, and Transposase (llumina). Samples were incubated for 30 minutes at 37C in a thermomixer at 1000rpm. Prior to amplification, samples were concentrated with the DNA Clean and Concentrator Kit-5 (Zymo). Libraries were prepared for sequencing using standard Illumina protocols