DNAseq of Cryptococcus gattii VGVI
Genomic DNA was extracted from cells grown in YPD (1% yeast extract, 2% peptone, 2% glucose) and washed twice in PBS using a DNeasy Plant Pro Kit (QIAgen, #69204). Cells were lysed with 0.5 mm zirconia/silica beads in a FastPrep 24 homogenizer (MP Biomedicals) for four cycles (30s at 6m/s followed by 90s chilling on ice). All other steps followed manufacturers instructions. Sequencing was performed by the Exeter Sequencing Service. Samples were prepared using the NEBNext Ultra II FS DNA Library Prep kit (NEB, #E7805) following manufacturers instructions. Libraries were loaded as part of a lane using XP loading on the NovaSeq 6000 using a paired-end strategy with a read length of 150 bases.