GSM8726433: NCIH226_JTE607; Homo sapiens; RNA-Seq
Samples were processed using the Chromium Single Cell 3′ (v2 Chemistry), platform with the target of ∼10,000 cells (10x Genomics) following manufacturer's instructions. Briefly, cells were added onto a chip to form Gel Bead-in-Emulsion (GEMs) in the Chromium instrument followed by cell lysis, barcoding, fragmentation, adaptor ligation and addition of sample index to the libraries before sequencing. cDNA from GEMs was recovered and amplified by PCR. Subsequently, cDNA fragment length was optimized by enzymatic digestion and fragment screening. Finally, library construction was completed by end repair and addition of traditional second-generation sequencing junctions, and a standard sequencing DNA library was obtained by PCR amplification. High-throughput sequencing was performed on the constructed library using the double-end sequencing mode of Illumina sequencing platform.