GSM9789614: 2B4/z, hCD45+mCD45-GFP+CD3+CD19-CD33-; Homo sapiens; RNA-Seq
Cells were sorted directly into TRIZOL LS for cell lysis and total RNA was extracted using the TAKARA SMARTer RNA extraction protocol (SMARTer Stranded Total RNA-Seq Kit, Takara Bio) according to the manufacturer's instructions. cDNA libraries were prepared using the TAKARA SMARTer Stranded Total RNA-Seq Kit (Takara Bio) and the high-output kit v2 (Illumina), following the manufacturer's protocol.