GSM9059600: MGC803 STAT1-K193R cells (Input); Homo sapiens; ChIP-Seq
Cells werefixed with1%formaldehydefor10minutes.After quenching and cell lysis, chromatin was sonicated using a E220focused-ultrasonicator(Covaris). ChlP-seq libraries were prepared using the KAPA HTP Library Preparation Kit complemented with NEXT flex DNABar codes from Bio Scientific.10 ng of DNA was used as starting material for input and ip samples.Libraries were amplified using 13 cycles on the thermocycler.Post amplification libraries were size selected at 250-450bp in length using Agencourt AMPure XP beads from Beckman Coulter. Libraries were validated using the Agilent High Sensitivity DNA Kit.