GSM8997246: Donor 1 scATAC-seq; Homo sapiens; ATAC-seq
Blood samples used were obtained from Versiti Ohio, Red Cross.  Natural killer (NK) cells were isolated from peripheral blood mononuclear cells (PBMCs) using the RosetteSep™ Human NK Cell Enrichment Cocktail (STEMCELL Technologies). Whole blood was first mixed with the RosetteSep™ NK cell enrichment cocktail, which selectively depletes unwanted cell populations by crosslinking them to red blood cells (RBCs). Following the addition of the cocktail, density gradient centrifugation was performed using Ficoll-Paque to separate enriched NK cells from the remaining blood components. Library preparation and quality control were subsequently performed according to the 10X Genomics scATAC-seq protocol.