GSM8681282: K+ FUS-bound RNA, rep 2; Homo sapiens; RNA-Seq
Total RNA was purified using the Qiagen RNeasy Mini Kit with RNase-free DNase set. RNA secondary structures were annealed in RIP buffer containing 150 mM of K+ or Li+, and annealed RNA was bound to recombinant C-terminal FUS protein. FUS-bound RNA was immunoprecipitated and pulled-down RNA purified with neutral phenol-chloroform. Input samples were subject to all steps as FUS-RNA samples except for immunoprecipitation and subsequent washes. cDNA libraries were prepared using the NEBNext Ultra II Directional Library Prep Kit for Illumina with the NEBNext rRNA Depletion Kit and Unique Dual Index Primer Pair Multiplex Oligos as per manufacterer's protocols.