rpoB amplicon sequences from environmental samples enriched for Ruegeria using specific primers: Yingkou sediment
Total bacterial DNA was extracted from environmental samples using CTAB Lysis Buffer (Nobleryder, Cat# N0212). The rpoB gene fragment was amplified with Ruegeria-specific primers, and the resulting libraries were sequenced on an Illumina NovaSeq platform to generate 250 bp paired-end reads.