Single nuclei RNA-seq on male hPSC-derived liver spheres using high energy diet
Nuclei were isolated using a TST-method from 3D liver spheres. Nuclei were selected via FACS using 7AAD stain. After sorting and counting, approximately 20,000 nuclei per sample were processed for single-nuclei sequencing using 10X Genomics 3' V3.1 reagents and following manufacturer instructions. Libraries were quantified using an Agilent Technologies TapeStation 2200 and sent for sequencing.