GSM9540351: KneeJoint-Control-3; Rattus norvegicus; RNA-Seq
Remove the medium and directly add 1000μL Trizol into the each hydrogel group to harvest samples. Total RNA was extracted using Trizol reagent (Sigma-Aldrich, USA) according to the manufacturer's instructions. The RNA purity and concentration was checked using the NanoPhotometer spectrophotometer (IMPLEN, CA, USA). A total amount of 1.5 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.