RNA-seq-ctrl5 (CRX2090206)
To investigate the role of actl6a in protonephros development and ciliary stability in zebrafish, we used morpholino microinjection to knock down actl6a in one-cell stage embryos (Na, K-ATPase alpha1A4:GFP, the transgenic line has GFP fluorescence in the pronephros). The treated embryos were cleaved and the tissue of pronephric duct was isolated.  We then perfomed gene expression profiling analysis using data obtain from RNA-seq of 10 different pronephric duct per group between control and knockdown. Comparative gene expression profiling analysis of RNA-seq data for pronephric duct of 30hpf ctrl morpholino injected embryos and actl6a knockdown embryos (ctrl and actl6a morphants)