Endothelial YAP/TAZ activation is a key mediator of atherosclerosis in Hutchinson-Gilford progeria syndrome
Endothelial YAP/TAZ activation is a key mediator of atherosclerosis in Hutchinson-Gilford progeria syndrome
Aortas (including aortic arch and thoracic aorta) from Lmna+/+ and LmnaG609G/G609G mice were dissected, cleaned of perivascular fat, and opened longitudinally.We analyzed two replicate samples per genotype, and each sample contained pooled cells from the aortas of two male and two female animals to avoid possible sex-related bias. Sorted cells were recounted and their viability checked on a Countess III cell counter (Thermofisher). Cells were loaded into each port of a Chromium Next GEM Chip G (10x Genomics) for single cell isolation and cDNA reverse transcription and amplification Sequencing libraries were created using the Next GEM Single cell 3â€™Library preparation kit v3.1 (10x Genomics) and indexed using the Chromium i7 Multiplex kit (10x Genomics) following the manufacturer instructions