PacBio HiFi sequences of Wachendorfia thyrsiflora: a right-morph individual
Genomic DNA for PacBio sequencing was isolated from 1 g of floral buds. Floral buds were ground to a fine powder with a pestle and mortar under liquid nitrogen, whereafter the frozen powder was resuspended in 5 ml high salt buffer I (50 mM EDTA, 0.16 M NaCl), and incubated at 50C for 10 min. The samples were centrifuged in a JA14 rotor at 10,000 g for 10 min at room temperature, whereafter the pellet was resuspended in 5 ml H1 Lysis buffer from the Macherey-Nagel NucleoBond HMW DNA kit. Genomic DNA was extracted according to the manufacturers instructions.