GSM9790465: A1_IgG_Positive_Rep1; Homo sapiens; RNA-Seq
After sorting, cell pellets were resuspended in 1 mL of Invitrogen™ TRIzol™ Reagent and stored at -80°C until RNA extraction. The Invitrogen™ PureLink™ RNA Mini Kit was used to extract and purify RNA from TRIzol™ following protocols outlined by the manufacturer. Optional on-column DNase treatment was performed. Purified samples were stored at -80°C until being transported on dry ice to the SKCCC Experimental and Computational Genomics Core for Bioanalyzer to assess RNA quality and bulk RNA sequencing. RNA sequencing libraries were prepared using the TruSeq Stranded Total RNA with RiboZero Human/Mouse/Rat” kit following the manufacturers instructions.