GSM9742739: PIC26 distal organoid region; Mus musculus; RNA-Seq
After in situ reverse transcription, selected regions were photo-irradiated at 365 nm for 3 min using a multi-pattern LED illumination system through a 20x objective. After photo-irradiation, specimens were lysed and cDNA:mRNA hybrids were purified using a Qiagen MinElute PCR Purification kit. Sequencing libraries were prepared from purified PIC cDNA:mRNA hybrids according to the previously reported PIC protocol. Distal and proximal libraries were indexed separately using UDI 174 and UDI 175, respectively, amplified by PCR and sequenced on an Illumina NovaSeq 6000 platform.