GSM9079337: whole organoids at d100; Homo sapiens; RNA-Seq
Organoid slices (n = 3) and organoids (n = 3), each pooled from the same biological replicate in one tube, were washed in cold PBS at 300xg, 5 min. After complete removal of PBS, organoid slices and organoids were placed on dry ice for 5 min, and then stored in liquid nitrogen. Preparation of samples for sequencing including the isolation of the nuclei, and initial quality control were performed as a service by GENEWIZ/AZENTA (USA). Library preparation, sequencing and initial data processing were performed as a service by GENEWIZ/AZENTA (USA). The RNA-seq library was prepared using 10X Genomics Chromium® 3` expression, and the 10X library sequencing was performed on the Illumina series. The target number of cells per sample was 6000, and number of reads per cell 50000.