N706C1 (CRX1311141; CRA021491)
The total DNA in soil samples was extracted using a Power Soil DNA Isolation Kit (MO BIO Laboratories, USA). After passing the genomic DNA test,the DNA was fragmented by mechanical interrupt method (ultrasonic), then DNA fragments experienced purification, ends repair, adding A to 3' ends and adding adaptors. Then agarose gel electrophoresis was used in fragment size selection and PCR amplification was used to construct the sequencing library. Once constructed libraries passed quality control, qualified libraries were sequenced on Illumina sequencing platform.