GSM9263641: MDA-MB-231-120h_Vehicle-B4; Homo sapiens; RNA-Seq
Total RNA extraction of cell-line samples was done by Qiagen RNeasy Mini Kit and quality checked by TapeStation. The extracted RNA was purified with oligo(dT) magnetic beads to capture mRNAs,fragmented and subject to a stranded RT proceeding. Library preparation protocol from Illumina Stranded mRNA Prep. Library QC included fluorometric quantification (Qubit) and fragment size distribution (TapeStation).