GSM9316237: csf1raMut;csf1rbMut, injured, replica 2; Danio rerio; RNA-Seq
Fish were dark adapted overnight, anesthetized in tricaine, and eyes removed and placed in a tray of ice-cold PBS (phosphate buffered saline). Retinas were isolated under a dissecting microscope using fine forceps. Retinal cells (10 retinas/genotype) were dissociated with the Papain Dissociation system (Worthington Biochemical).  Cells were passed through a 30 micron filter to remove cell clumps. Libraries were created using Chromium Single Cell 3' reagent kit (v3.1) according to manufacturer's instructions.