GSM9500971: AdCL+Spn day 21, sample 4; Mus musculus; RNA-Seq
One hundred ng of total RNA per sample were utilized as input for mRNA enrichment procedure with 'NEBNext® Poly(A) mRNA Magnetic Isolation Module' (E7490L; New England Biolabs, NEB) followed by stranded cDNA library generation using 'NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina' (E7760L; NEB), according to the user manual E7760 (Version 1.0_02-2017) except that all reactions were downscaled to 2/3 of initial volumes. One additional purification step was introduced at the end of the standard procedure, using 1x 'Agencourt® AMPure® XP Beads' (#A63881; Beckman Coulter). 300ng of total RNA per sample were utilized as input for mRNA enrichment procedure with 'NEBNext® Poly(A) mRNA Magnetic Isolation Module' (E7490L; New England Biolabs) followed by stranded cDNA library generation using 'NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina' (E7760L; New England Biolabs). All steps were performed as recommended in user manualE7760 (Version 1.0_02-2017; NEB) except that all reactions were downscaled to 2/3 of initial volumes.