E.coli E5-DE5
Overnight cultures of E. coli E5 were diluted 1:100 (v/v) into fresh LB medium and incubated at 37C with shaking at 220 rpm until OD reached 0.8. Phage DE5 was added at an MOI of 0.01, and incubation was continued at 37C and 220 rpm for an additional 3 h