Bulk RNA-seq of OLIVe FRET-low population from MDA-MB-231 xenograft rep1
Total RNA was isolated from FACS-sorted MDA-MB-231 OLIVe-expressing cells dissociated from xenograft tumor. cDNA was synthesized and amplified following the Quartz-Seq protocol (Sasagawa Y et al., Genome Biol. 2013;14(4):R31, PMID: 23594475), with modifications for bulk RNA input, which selectively reverse-transcribes poly(A)+ mRNA using oligo-dT priming. Sequencing libraries were prepared from the amplified cDNA using KAPA Hyper Prep Kit (Roche/KAPA Biosystems) with SeqCap Adapter Kit A (Roche, cat. 7141530001) for adapter ligation and indexing. Mean fragment size: 333 bp.