GSM8795438: PDCC,DMSO,rep1; Homo sapiens; RNA-Seq
300 GFP-positive cells and 10,000 GFP-positive cells were sorted by FACS for small scale RNA-seq and ATAC-seq. 10% HEK-293T cells were mixed in ATAC-seq samples for spike-in normalization. ATAC-seq was performed with Chromatin Profile Kit for Illumina (Novoprotein, N248-01A). Small scale RNA-seq library was constructed using Smart-seq2 method with modifications allowing higher quality library generation from pancreatic cells. Briefly, mouse pancreatic cells were directly sorted into 8 μl of denaturing lysis buffer (6 M guanidine HCl, 0.2% (vol/vol) Triton X-100 and 1% (vol/vol) β-mercaptoethanol). RNA SPRI beads (RNA Clean XP beads, Beckman A63987) were added (2.2X) to the lysis buffer and RNA was purified, followed by library preparation by the standard Smart-seq2 protocol, except with a 12-cycle PCR pre-amplification step. Smart-seq2 protocol.