GSM9685779: MPAL 50891_1566268; Mus musculus; RNA-Seq
Mouse genomic DNA and RNA were extracted from sorted MPAL or lineage control cells following the direction of the AllPrep DNA/RNA Mini kit (Qiagen, #80204) and stored at -80°C until the sequencing. RNA sequencing libraries were prepared with Kapa RNA mRNA HyperPrep kit (Kapa Biosystems, Cat KR1352) according to the manufacturer's protocol. The final libraries were validated with the Agilent Bioanalyzer DNA High Sensitivity Kit and quantified with Qubit and sequenced on Illumina NovaSeq6000 S4 flow cell.