Genomic raw reads for Erwinia persicina P4.1
Genomic DNA from Erwinia persicina P4.1 was extracted and assessed for quality and quantity using standard methods. Sequencing libraries were prepared using the Illumina TruSeq DNA PCR-Free Library Preparation Kit following the manufacturers protocol. Briefly, high-quality genomic DNA was mechanically fragmented to an average insert size of ~350 bp. Fragmented DNA underwent end-repair and A-tailing, followed by ligation of Illumina sequencing adapters containing sample-specific indices. Adapter-ligated DNA fragments were purified and size-selected using magnetic bead-based cleanup to obtain the desired insert size. As the protocol is PCR-free, no amplification step was performed. Library quality and fragment size distribution were assessed using a bioanalyzer or equivalent, and concentrations were quantified prior to sequencing. The libraries were sequenced on an Illumina NovaSeq 6000 platform using paired-end reads (2 x 150 bp).