Microbiome of Tomato field microbiome
Genomic DNA was extracted from 150 to 200 mg of cecal contents using the PureLink Microbiome DNA Purification Kit (Life Technologies, Invitrogen Corp.), combined with RNAse treatment (10 units/hr). After quality control with electrophoresis and nanodrop,  the 16S rRNA V4-V5 variable region was amplified, purified, and sequenced. Amplicon libraries were prepared by using Phusion High-Fidelity PCR Kit (New England Biolabs Inc, Ipswich, MA, USA). PCR products were cleaned using AMPure XP PCR (Beckman Coulter Inc, Beverly MA, USA) and sequenced using Illumina MiSeq 300-base paired-end kit at the Molecular and Cellular Imaging Center (https://mcic.osu.edu/)