Allele frequency changs in temporal populations of two timothy cultivars
Allele frequency changs in temporal populations of two timothy cultivars
For population genetic studies, approximately 80–100 seeds of each population were sown in two pots and cultivated at ambient room temperatures for 8 weeks. For DNA extraction, approximately an equal amount of tissue from 30 randomly selected plants from each pot was collected and flash-frozen in liquid nitrogen. Plants were grown for 8 weeks at ambient room temperature in green houses DNA extractions were carried out using Promega wizard HMW DNA extraction kit according to the manufacturer's guidelines restriction enzymes - RsaI and HaeIII were choosen to uniformly cover the genome based on pre-experiment 