Exome capture of disease enriched cells from human acute myeloid leukemia patients (T = tumor samples; G = germline samples)
Next generation sequencing libraries were prepared from 50ng input DNA using Agilent SureSelectXT V4 51mb Exome kit per manufacturer's recommendations. Sequencing was performed on an Illumina HiSeq 2500 using 125bp paired-end sequencing to a mean depth per target area of 94.6X (for tumors) and 91.6X (for matched germline samples)