GSM9078364: ISLET1_4C_NT_HH25; Gallus gallus; OTHER
Cells were dissociated in Accumax for 20min at RT under mild agitation. CUT&RUN experiments were carried out as previously described (Rothstein and Simoes-Costa, 2019). CUT&RUN libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, E7645) and sequenced with paired-end 50bp reads on an Illumina NextSeq1000 instrument.