GSM8690361: Binf_EBV_dEBNALP_48h_rep4; Homo sapiens; RNA-Seq
Total RNA was extracted using the Direct-zolTM RNA MicroPrep Kit (Zymo Research) RNA libraries for RNAseq were prepared by Novogen: messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.