bulk RNA-Seq of Mus musculus: adult male circumvallate taste papillae
10 m thick coronal sections of fresh frozen tongue from C57BL/6 mice containing CVP were prepared using a cryostat. Sections were stained with hematoxylin eosin, and pools of ~100 taste buds from CVP (n=2 pools from 2 mice) were excised using laser micro dissection, and full length bulk RNASeq libraries were prepared using the Ovation RNASeq system V2 (Tecan biosystems, Morgan Hill, CA) per manufacturer instructions.