RNA-Seq of Mesocricetus auratus: old male lung
Total RNA was extracted from hamster lung tissues or blood using TRIzol reagent. mRNA was enriched using oligo(dT) magnetic beads, fragmented, and used for first-strand cDNA synthesis with random hexamer primers, followed by second-strand synthesis. The libraries were constructed through end repair, A-tailing, adapter ligation, PCR amplification, and purification.