whole-geno-seq-Vmo-F-HIC (CRX763837; CRA012208)
The leaves of the Vernicia montana were fixed and crosslinked with 2% formaldehyde. DNA was cut with DpnII over-night in 37 degrees centigrade, cohesive ends were filled with Klenow polymerase and marked with biotin-14-dCTP, then blunt end fragments were ligated by using T4 DNA ligase for 4 h in 16 degrees centigrade. The ligation products were purified and DNA were sheared into 300 to 500 bp, biotin-containing fragments were finally used to create pair-end sequencing libraries.