GSM9323176: P05; Homo sapiens; RNA-Seq
Frozen tissues were homogenized using liquid nitrogen-cooled grinders. Total RNA was extracted using the Qiagen RNeasy kit (Qiagen, cat#74104, Germany), followed by DNase treatment to eliminate genomic DNA. RNA quality was assessed by spectrophotometry (A260/A280 ratio ≥1.8) and a bioanalyzer (RNA Integrity Number,RIN ≥7) Libraries were amplified using TruSeq™ RNA and DNA Library Preparation Kits v2 (Illumina, cat# RS-122-2001, USA),and RNA sequencing.