16S ampl seq of soil
Libraries were prepared by following Illumina 16S Metagenomic Sequencing Library Preparation protocol in two amplification steps: an initial PCR amplification using 16S locus-specific PCR primers, and a subsequent amplification that integrates relevant flow-cell binding domains and unique indices (NexteraXT Index Kit, FC1311001/FC1311002). Libraries were sequenced on NovaSeq 6000 instrument (Illumina, San Diego, CA) using 250-bp paired-end mode.