WGS of Lepidochaetus zelinkai
Ethanol-preserved individuals were air-dried to remove residual ethanol, resuspended in PBS, and subjected to whole genome amplification (WGA) using the REPLI-g Single Cell Kit (QIAGEN) according to the manufacturers instructions. Amplified DNA was validated by PCR amplification and Sanger sequencing of the 18S rDNA gene. The resulting sequences were compared to GenBank references using BLAST to confirm taxonomic identity. Verified WGA products were used for library preparation with the TruSeq DNA PCR-Free Kit and sequenced on an Illumina NovaSeq 6000 platform (2  150 bp), generating approximately 40 million paired-end reads per sample.