GSM8465141: Distal -cAMP HIOs _2; Homo sapiens; RNA-Seq
The cells were dissociated to a single cell suspension using 0.05% and subsequently sorted for live cells using Calcein blue and CDX2 GFP positivity. Single Cell RNA sequencing was then preformed using the Chromium Single Cell 3'system according to manufacturers' instructions (10x Genomics) at the Single Cell Sequencing Core at Boston University Medical Center. All independent differentiations were expanded in parallel and sequenced at day 66 using the 10x Chromium platform on the same day to avoid technical batch effects Day 66 library generated Distal HIO (1) 2,705 cells l and Distal HIO (2) 3,342 cells. We analyzed Distal HIOs in the absence of cAMP (1) 2,619 cells and Distal HIOs in the absence of cAMP (2) 3,038 cells. We analyzed Proximal HIOs  (1) 420  cells and Proximal HIOs (2) 1,711 cells.