RNA-seq of Caulerpa racemosa
Caulerpa tissue was rinsed with synthetic seawater and manually cleaned to remove debris and other cell types. This material was then blotted dry with paper towels, flash frozen in liquid nitrogen, ground to a fine powder using a pre-cooled mortar and pestle and stored at -80 C. RNA was extracted and samples were prepared into cDNA with a polyA enrichment and poly-T primers for reverse transcription. Libraries were prepared and sequencing using BGIs cPAS and DNA nanoballs technology.