RNA Seq of Raw264.7 macrophages
Total RNAs from RAW264.7 macrophages were extracted using Norgen BiotekTM total RNA purification kit (Alkali Scientific, Fort Lauderdale, FL, USA). DNase treatment and RNA cleanup were performed on the extracted RNA samples to generate high RNA quality with RNA integrity number between 9.70  10. Following the extraction, the RNA samples were QCed, pooled, used to construct NGS library, and sequenced using Illumina NovaSeq X series sequencing machine (Illumina, Inc., San Diego, CA, USA)