GSM8516060: Xenopus embryo, stage 35, 0.75X MMR, sample 3; Xenopus laevis; RNA-Seq
Each sample (0.1X MMR and 0.75X MMR) of Xenopus embryos consisted of 10 pooled stage 35 Xenopus embryos and each was repeated 3 times. RNA was extracted using TRI Reagent (Molecular Research Center, Inc - MRC) as per the manufacturer's protocol, and total RNA quality and quantity was assessed using a NanoDrop spectrophotometer (Thermo Fisher Scientific). RNA library preparation was done with the Illumina Stranded Total RNA with Ribo-Zero Plus. Libraries were then multiplexed, and an rRNA depletion run using single-end, 75-nucleotide sequencing was performed on Illumina HiSeq 2500.