BY606-001R0013 (CRX1862838; CRA028005)
Microbial DNA was extracted from cecal content using a CTAB-based method. DNA quality and quantity were assessed using a Qubit 3.0 Fluorometer (Invitrogen). Four ZF samples were excluded due to insufficient DNA yield, resulting in 36 samples (20 CF, 16 ZF) for library preparation. Libraries were constructed using the VAHTS Universal Plus DNA Library Prep Kit (Illumina, San Diego, CA, USA) and sequenced (2 x 150 bp) on an Illumina NovaSeq X platform following standard protocols. submitted_library_selection: DNAse