GSM9826426: Pressure-trained ECT (MCTR_Endo_plus); Homo sapiens; RNA-Seq
ECTs were dissociated into single-cell suspensions using enzymatic digestion. Viable cells were collected and used for single-cell RNA sequencing library preparation. Single-cell RNA-seq libraries were prepared using the Chromium Single Cell 3' Gene Expression platform (10x Genomics) according to the manufacturer's instructions and sequenced on an Illumina NovaSeq 6000 platform.