WGS of Homo sapiens gut microbiome
DNA libraries were prepared using the Watchmaker DNA Library Prep Kit (7K0019-1K). Genomic DNA was fragmented using a mastermix of Watchmaker Frag/AT Buffer and Frag/AT Enzyme Mix. IDT xGen UDI Primers and IDT Stubby Adapters were added to each sample followed by 7 cycles of PCR to construct the DNA libraries. The final DNA libraries were purified using CleanNGS magnetic beads (CleanNA) and eluted in nuclease-free water. Following elution, the libraries were quantified using the Qubit fluorometer dsDNA HS Assay Kit. Libraries were then circularized using the Element Adept library compatibility workflow and sequenced on the Element AVITI platform using the AVITI 2x150 Cloudbreak sequencing kit.