Bisulfite-Seq of Zea mays WT Repeat3
Genome-wide bisulfite sequencing (WGBS) was performed to methylate the DNA landscape of maize (Zea mays) under SGD102 gene overexpression. Leaf samples were collected from maize at the sixth- and seventh-leaf stages, including both control (CK) and overexpression (OE) groups. Genomic DNA was extracted using a plant DNA extraction kit, and bisulfite conversion was performed using the EZ DNA Methylation-Gold Kit (Zymo Research). The libraries were constructed using the Illumina TruSeq DNA Methylation Kit and sequenced on an Illumina NovaSeq 6000 platform with 150-bp paired-end reads. Quality control, adapter trimming, and methylation analysis were conducted using FastQC, Trim Galore, and Bismark.