Amplicon sequencing of Escherichia coli: LasR binding site library, replicate 3, FACS bin 2
Sort-seq of a randomized LasR binding site library in Escherichia coli DH10B. Cells carrying the plasmid-borne variant library were sorted by FACS into eight fluorescence bins; bin-specific barcoded amplicons were pooled into a single paired-end Illumina library. Reads submitted here are the per-bin reads obtained after demultiplexing, primer removal, and merging of read pairs with FLASH v2.2.00 (minimum overlap 10 bp). This sample corresponds to one fluorescence bin of one biological replicate.