PacBio Hi-Fi sequencing of Uta stansburiana; adult male liver
The lizard was euthanized following IACUC protocol (University of California Merced AUP21-0001) and liver tissue was immediately removed. Half of the tissue was used in immediate DNA extraction. The remains of the individual were formalin-fixed and prepared for museum donation (to be deposited at the Museum of Vertebrate Zoology, DLE3124). Genomic DNA was extracted using a modified buffered lysis and detergent salting-out protocol from Moore et al (2004) and Macdonald et al (2011). We assessed sample purity using a NanoDrop 1.0 and quantified samples using the Qubit 2.0 high sensitivity kit. The extracted DNA was sent to the University of California Davis DNA Technologies and Expression Analysis core and sequenced using two Pacific Biosciences Sequel II SMRT-cells run in CCS mode.