Whole-genome sequencing of canine oral melanoma and matched peripheral blood pre- and post-radiotherapy
Whole-genome sequencing of canine oral melanoma and matched peripheral blood pre- and post-radiotherapy
Tumor tissue was obtained by Tru-cut biopsy and preserved in RNAlater™ before RT. Peripheral blood was drawn from the external jugular vein into Tempus™ Blood RNA Tubes at two time points: before RT (Pre-RT) and immediately after the final radiation fraction (Post-RT 0m). Samples were stored at −80 °C until processing. Animal procedures were approved by the IACUC (GNU-240826-D0172). Genomic DNA was extracted from Pre-RT Tumor, Pre-RT Blood, and Post-RT 0 m Blood by the service provider following internal standard operating procedures. PCR-free whole-genome libraries were prepared using the TruSeq PCR-free Sample Preparation Kit (Illumina). Library QC was performed with KAPA Library Quantification and Agilent High Sensitivity DNA chip.