GSM9435894: HC3; Homo sapiens; RNA-Seq
Fresh-frozen human ileal biopsy sample were processed according to the manufacturer's demonstrated protocol for tissue fixation and dissociation (CG000553, RevB). Briefly, tissues were weighed and minced on ice using a pre-chilled glass petri dish, followed by fixation in Fixation Buffer containing 4% formaldehyde for 16–24 hours at 4 °C without agitation. Fixed tissues were washed and resuspended in Quenching Buffer, then stored at –80 °C. Upon thawing, fixed tissues were enzymatically dissociated using pre-warmed Dissociation Solution containing Liberase TL and processed with a gentleMACS Octo Dissociator (Miltenyi Biotec). The resulting single-cell suspensions were filtered through a 30 µm strainer. Fixed cells were then subjected to probe hybridization with the Chromium Human Transcriptome Probe Set v1.0.1, and barcoded using the Chromium X instrument. Libraries were generated with the Chromium Fixed RNA Profiling Reagent Kit and sequenced on an Illumina NovaSeq X Plus system.