GSM8726419: Whole BM, ko, Mouse 1; Mus musculus; RNA-Seq
Whole BM cells were harvested by flushing of femora and tibiae and viably frozen. Viably frozen cells were thawed at 37 ºC, resuspended in ice-cold PBS, washed twice and counted with a LUNA automated cell counter (Logos Biosystems). Single cell capture, reverse transcription and library preparation were carried out on the Chromium platform (10x Genomics) with the single cell 3ʹ reagent v2 protocol according to the manufacturer's recommendations using 1,000 cells as input per reaction channel. The cDNA libraries were paired end sequenced (26 bp and 74 bp) on the Illumina HiSeq 4000 system.