Sequencing of whole genome amplification product of Acanthechiniscus victor with Illumina
A whole individual was subjected to WGA with Qiagen REPLI-g single cell advanced kit. CeleroTM DNA-Seq Library Preparation kit (Tecan Genomics, Redwood City, CA) has been used for library preparation following the manufacturers instructions. Both input and final library were quantified by Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA) and quality tested by Agilent 2100 Bioanalyzer High Sensitivity DNA assay (Agilent technologies, Santa Clara, CA). Libraries were then prepared for sequencing and sequenced on NovaSeq X in paired-end 150 bp mode.