GSM9107603: RNA-seq of P. ostreatus CK replicate 3; Pleurotus ostreatus; RNA-Seq
Total RNA was extracted using TRIzol reagent (Invitrogen) following the manufacturer's instructions. RNA quality was confirmed using NanoDrop and Bioanalyzer 2100 (RIN > 7.0). Poly(A) mRNA was enriched using Dynabeads Oligo(dT) and fragmented at 94 °C for 5–7 min. Libraries were prepared using SuperScript II, dUTP-based second-strand synthesis, and UDG digestion. Libraries were size-selected (~300 bp), PCR-amplified (8 cycles), and sequenced on an Illumina NovaSeq 6000 using 2×150 bp paired-end mode.