Amplicon sequencing of soil metagenomes: agricultural soil under perennial-forage and annual crop rotations
Soil DNA was extracted using the Qiagen DNeasy PowerLyzer PowerSoil kit, following the manufacturers instructions. Libraries targeting the 16S rRNA gene (v4/v5 region) and fungal ITS region were prepared following Qiagen protocols. Sequencing was performed on an Illumina MiSeq platform at the Centre for Health Genomics and Informatics, University of Calgary.