GSM9574729: PC-3 cells, parental; Homo sapiens; RNA-Seq
Total RNA from both parental PC-3 cells and clone #1 cells, isolated from cells grown to 70% confluence, underwent next-generation sequencing (NGS) to identify differentially expressed genes influenced by LCN2 deficiency. The RNA samples were initially assessed for concentration, purity, and integrity using UV spectrophotometry and an Agilent 4200 TapeStation (Agilent Technologies Inc., Waldbronn, Germany). After removal of ribosomal RNA, the remaining mRNA fraction was converted into a sequencing library using the NEBNext® Multiplex Oligos for Illumina® Index Primers Set 1 (#E7335S, New England Biolabs, Frankfurt am Main, Germany). These libraries were then sequenced on an Illumina instrument using 300-cycle MiSeq Reagent kit V2 cartridges (Illumina Inc., San Diego, CA, USA). All cDNA library preparations and sequencing procedures were conducted at the IZKF Genomic Facility, University Hospital Aachen