GSM8578151: 3XFLAG-LEDGF-1-325_IP_3; Homo sapiens; ChIP-Seq
Antibodies for ChIPSeq: FLAG (Sigma; Cat# F1804), RNA Pol II (8WG16 BioLegend; Cat# 664912), H3K4me3 (C42D8 Rabbit mAb #9751 cell signaling), H3K36me3 (Invitrogen; MA5-24687), and MLL1 (Rabbit anti-MLL1 antibody Bethyl; Cat#A300-374A). ChIP-Seq libraries were constructed from 40 ng of ChIP DNA using Ovation Ultralow System V2 1-96 (Tecan) with 15 cycles of PCR amplification. The final libraries were twice purified using Ampure XP PCR Purification Beads (Agencourt). The pooled libraries were sequenced on a NovaSeq 6000 (Illumina) using version 1.5 chemistry to achieve approximately 80 million 151-base read pairs. The data was processed using RTA version 3.4.4.