WGS of M.tb with mmpr5 mutation
Isolates for whole genome sequencing were cultured in MGIT media with PANTA (Becton Dickinson, NJ, USA) from stored glycerol stocks before culture in Middlebrook 7H9 media supplemented with OADC (Becton Dickinson, NJ, USA). The DNA was extracted using the CTAB method and quantified using a Qubit 4 Fluorometer with the 1x dsDNA HS Qubit Assay (Life Technologies, CA, United States). One hundred nanograms (100 ng) of extracted template DNA was used for library preparation using the Illumina DNA prep library kit (Illumina, CA, USA). Sequencing was conducted on an Illumina MiniSeq with 149-bp paired-end reads.