GSM9316679: J-H3K9bhb-shBDH1-IP-2; Homo sapiens; ChIP-Seq
Jurkat cells were transfected with lentivirus expressing control or BDH1 shRNA, and puromycin selection (2 μg/mL) for 48 h. Jurkat cells were initially crosslinked with 1% paraformaldehyde for 10 min and then were quenched with glycine for 5 min. After cell lysis, fixed chromatin underwent fragmentation to achieve DNA sizes ranging from 200 to 600 bp utilizing a DiagenodeBruptor Plus DNA fragments was immunoprecipitated with indicated antibodies and Protein A/G beads (Smart-Lifesciences). 1-10 ng of immunoprecipitated DNA was employed for DNA library preparation.