GSM9110423: placental tissues, 13.5 dpc, mother 3; Monodelphis domestica; RNA-Seq
Placental-only transcriptomes were captured from laser microdissected samples of fetal tissues directly apposing the uterine lumen in cryosections of 13.5 dpc pregnant uteri. Uteri to be used for this procedure were dissected into phosphate-buffered saline and subsequently immersed Tissue-Tek OCT compound (Sakura Finetek, 4583) in a block mold and flash frozen by exposure to a bath of isopentane surrounded by dry ice, and stored at -80°C. 14-μm sections were prepared from the resulting blocks on a cryomicrotome (Microm HM 500 OM) and placed onto polyethylene naphthalate membrane slides (Leica Microsystems, 11505158) which had been sterilized by ultraviolet irradiation in a biosafety cabinet (Baker SterilGARD, SG-404) for 30 minutes. Sections were processed on a Leica LMD7000 apparatus and excised tissue dropped directly into lysis buffer for processing using a Qiagen RNeasy Micro Kit (74004). RNA libraries were prepared by the Yale Center for Genomic Analysis using a NEBNext Low Input Library Prep Kit (E6420).