GSM9314814: fecal, GMT, 12-15wk, rep1; Mus musculus; OTHER
Genomic DNA (200 ng) was randomly sheared into ~350 bp fragments using a Covaris ultrasonic disruptor. The integrity and insert size distribution of the resulting libraries were assessed with AATI analysis. Libraries meeting the expected insert size were further quantified using quantitative PCR (qPCR), and only those with an effective concentration greater than 3 nM were retained.