GSM9758698: Tau-6; Mus musculus; RNA-Seq
10 mg of frozen prefrontal cortex was dissociated in RLT buffer (#74106, Qiagen RNeasy Mini Kit, Germany) with 10% beta-mercaptoethanol and mRNA was isolated using the Norgen MicroRNA Isolation Kit (#21300, Canada) according to manufacturer's protocol. mRNA libraries were generated using the SMART-Seq mRNA Kit (#634773, Takara, CA) combined with the Nextera XT DNA Library Preparation Kit (#FC-131-1096, Illumina, CA) and Illumina DNA/RNA UD Indexes Set A (#20091654, Illumina, CA).