GSM8465362: PANC1PSA_PS03ULAPANC1PSAS285_GTCTCGATC_L003_R1_001, PANC1, Cobimetinib-VSP34I; Homo sapiens; OTHER
After 24 h of incubation, the plates were treated with following drugs: RAF inhibitors – Sorafenib, Dabrafenib, RAF709, PLX8394, GDC-0879; MEK inhibitors – Trametinib, Cobimetinib, Binimetinib, Selumetinib, Rafametinib; and ERK inhibitors – SCH772984, Ulixertinib, AZD0364, Ravoxertinib; in combination with Autophagy inhibitors: Hydroxychloroquine or Compound 19 (Vps34 inhibitor). Each drug was dosed at the concentration at which the cells were 80% viable after 48 h of treatment with monotherapy. After 24 h of treatment, the medium was replaced with 100 ml of FBS supplemented with 10% DMSO and the plates were frozen at −80 °C prior to PLATE-Seq. Detailed protocol for preparation of the automated PLATE-SEQ experiment was described by Bush et al. (Bush et al., 2017). Samples were sequenced using PlateSeq approach