WGS of greenhouse soil :bacteria and fungi
The workflow of DNA library preparation using the NEBNext Ultra DNA Library Prep Kit is as follows: First, qualified DNA samples are fragmented to 350bp using the Covaris sonicator. Then, the fragmented DNA undergoes end-repairing, A-tailing, adapter ligation, size selection, PCR amplification and purification. After that, the library is amplified again. The PCR products are purified with the AMPureXP system and the library quality is assessed using the Agilent5400 system. Finally, the library concentration is quantified by QPCR to 1.5 nM.