Permafrost coprolite ancient environmental DNA
SedaDNA based extraction (cold spin + high-volume binding buffer, Murchie et al. 2021), double stranded library prep (Meyer and Kircher 2010), dual indexing (Kircher 2012), capture enrichment (Murchie et al. 2021), pooling, size selection via gel excision (150-500 bp), sequencing Illumina NextSeq