UCE target enrichment of Gnamptogenys_xalapa_EX2701: adult worker
Samples were extracted using column-based extraction kits. DNA was sheared to 400-600 bp using a DNA sonicator. Sheared DNA was then shipped to Rapid Genomics (Gainesville, FL) for library preparation, target enrichment, and Illumina sequencing. Ultraconserved elements were enriched using an ant-customized RNA bait set (Arbor Biosciences; ant-specific hym-v2) that includes 9,898 baits (120 mer) targeting 2,524 UCE loci shared across Hymenoptera and a set of legacy markers (Branstetter et al. 2017). Approximately two million paired-end reads of enriched library and one million pair-end reads of unenriched library (WGS) were generated using Illumina NovaSeq or equivalent platforms (2x150 bp). The enriched and unenriched reads were combined into single R1 and R2 read files prior to upload to the SRA.