Treatment
Each of the sample is pooled from equal amount of RNA extracted at three time points, amount of, 7th day 14th day and 21st days after treatment with control (N+) or nitrogen deficiency leaf and root tissues separately using mIRVANA kit. After quantification, equal amount of small RNA from leaf and root samples from three time points were pooled before sending for sequencing. In effect each sample is pooled from 6 biological replicates