GSM8691148: NKCLR3 RAT-seq in NK-92MI; Homo sapiens; OTHER
For RAT-Seq, NK-92MI cells were first cross-linked to fix the lncRNA-chromatin structure. The regulatory element-interacting RNAs were reverse transcribed in situ in the nucleus using lncRNA-specific complementary primers in a reaction mixture containing biotin-dCTP. The RNA-biotin cDNA chromatin complex was pulled down with streptavidin beads and the target gene DNAs were purified for library sequencing. As the RAT control, RAT control dataset was generated by random oligo primers. RAT-DNA libraries were prepared using Illumina's Kit (Part# 0801-0303).