GSM8464514: HCT116 cells, SDR42E1 overexpression, biol rep3; Homo sapiens; RNA-Seq
Total RNA was isolated from cell lines using 1 mL TRIzol reagent (Invitrogen), according to the manufacturer's guidelines. Subsequently, equivalent amounts of high-quality RNA pools (100–200 ng) were precipitated in a 75% ethanol solution and shipped to Macrogen Inc. (http://macrogen.com, Korea) for total RNA-sequencing library construction and next-generation sequencing. RNA sequencing libraries were prepared with the TruSeq Stranded Total RNA Library Prep Kit (1000000040499, Illumina), following the instructions.