GSM9541974: conventional EGFRvIII targeting CAR-T cell-rep 2, 0.5 mM glucose condition, day 7; Homo sapiens; RNA-Seq
RNA was extracted using an RNeasy kit according to the manufacturer's instructions (QIAGEN, Hilden, Germany). 1.0μg of total RNA was used for the construction of sequencing libraries. Sample 1-4: Libraries were prepared using theNEBNext Ultra II Directional RNA Library Prep Kit  (New England BioLabs, Ipswich, MA) Sample 5-16: Libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit (New England BioLabs, Ipswich, MA)