WGS of Plasmodium falciparum MT058
Using DNA extracted from leukocyte-depleted samples, genomic DNA was enzymatically fragmented (NEBNext Ultra II FS DNA Library Prep Kit for Illumina). Samples were quantified with a fluorometric method (NEBNext Library Quant Kit for Illumina), diluted to a standard concentration (4 nM), and sequenced on the Illumina NextSeq500 platform using a NextSeq mid-out kit (300 cycles), following the manufacturers standard protocol. For each sample, 2x150 base pair paired-end reads were generated.