GSM9541030: Parse_Rat_SS_BAT; Rattus norvegicus; RNA-Seq
Nuclei from Dahl SS rat and Holstein dairy cow adipose tissue were isolated and processed using a custom protocol outlined in the associated publication. The protocol is similar to the protocol found here: Whytock, K. L. et. al. (2023). Isolation of nuclei from frozen human subcutaneous adipose tissue for full-length single-nuclei transcriptional profiling. STAR Protocols, 4(1), 102054. https://doi.org/10.1016/j.xpro.2023.102054 The 10X Chromium Next GEM Single Cell 3' Reagent Kit v 3.1 (Dual Index) was used with a targeted cell recovery of 10,000 according to manufacturer's recommended protocol (CG000315, Rev D).