GSM9686524: Mouse SGZ NSCs, 0.8 μM CdCl₂, biol rep 1; Mus musculus; RNA-Seq
Total RNA was extracted using TRIzol reagent (Tiangen) according to the manufacturer's instructions. RNA quality and quantity were assessed before library preparation. RNA-seq libraries were prepared using standard Illumina protocols by Shanghai OE Biotech Co., Ltd. Briefly, mRNA was enriched, fragmented, and reverse transcribed into cDNA. Libraries were amplified and purified, then sequenced on an Illumina platform.