GSM9615767: WTZT10_IN; Homo sapiens; ChIP-Seq
Total RNA was extracted from all samples using the TRIzol-phenol-chloroform method. Total RNA was extracted from the samples. Ribosomal RNA (rRNA) was depleted from the total RNA using specific probes to retain both mRNA and non-coding RNA. The rRNA-depleted RNA was then fragmented. First-strand cDNA was synthesized using random hexamer primers and reverse transcriptase, followed by second-strand cDNA synthesis. The double-stranded cDNA underwent end repair, A-tailing, and ligation of sequencing adapters. The final sequencing library was enriched by PCR. Library quality, including concentration and fragment size, was evaluated using a Qubit fluorometer and an Agilent 2100 Bioanalyzer before sequencing on the Illumina platform.