WntR_Rep1
The genomic DNA was extracted from the WNT-BC1 INSCRIBE cells using the Genomic DNA Purification Kit (NEB, T3010) according to the manufacturer's instructions. The targeted region was amplified from collected genomic DNA or reference plasmid using high fidelity Herculase II Fusion DNA Polymerase (Agilent, 600675). The input template for PCR was 100 ng of genomic DNA or 1 ng of reference plasmid in a final reaction volume of 50 l. The samples were incubated for 2 min at 95 C; 30 s at 95 C, 30 s at 56 C and 60 s at 72 C for 36 cycles; and 10 min at 72 C. Amplicon libraries contained the barcode arrays with an extra 50bp on each side and were sequenced by the Plasmidsaurus Premium PCR sequencing service, which is based on nanopore long-read sequencing.