GSM8968932: Young 72h fasted biol rep 2; Nothobranchius furzeri; RNA-Seq
Harvested tissues were snap-frozen in liquid nitrogen and stored at –80 °C. RNA extraction of all samples was done at the same time. About 1 μg of total RNA was used per sample for library preparation. The ribosome removal step was conducted using the RiboZero rRNA removal kit (Illumina).