Nanopore adaptive sampling of Ixodes scapularis: Whole adult female
We leveraged total genomic DNA from PCR ascertained infected and uninfected blacklegged ticks. Total genomic DNA from 168 adult blacklegged ticks were assigned to one of seven sequencing experiments, where each experiment comprised 24 barcoded and pooled ticks. Nanopore adaptive sampling enriched for bacterial pathogens vectored by blacklegged ticks, and this dataset includes raw FASTQ files from each sequenced tick. Library selection was performed by DNA fragmentation, end repair and adapter ligation.