WGS of Lactococcus garvieae SJC17
The NGS sequencing project started with quality control of all the samples, which included the analysis of concentration and integrity of DNA. The library construction was carried out using the Whole Genome Sequencing prep kit (based on Kapa HyperPrep kit). The generated DNA fragments (DNA libraries) were sequenced in the lllumina Novaseq, using 150bp paired-end sequencing reads and the quality control of raw data generated was performed. Integrity: 1.5% agarose gel electrophoresis in TAE buffer; Concentration: Qubit 4.0 fluorometer and Qubit dsDNA BR kit. Quality control post-sequencing was performed by FastQC software.