GSM8765730: MC38_L_rep3; Mus musculus; RNA-Seq
RNA was extracted with Norgen Single Cell RNA Purification Kit and RNA integrity and concentration was assessed with Nanodrop Library preparation was performed with Nextflex Rapid Directional RNA-Seq kit 2.0 Kit with Poly(A) Beads 2.0 and sequencing was performed with Illumina Novaseq 6000 platform. The resulting raw reads were assessed for quality by FastQC. The mean per-base quality score (Phred-score) was consistently above 35 for all samples.