GSM9436842: Female_Brain_0PCB_YesFA_18_02; Mus musculus; RNA-Seq
Nucleic acids were extracted by homogenizing the same half of placenta and brain tissue using a TissueLyser II (Qiagen) followed by the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen) according to the manufacturer's instructions. Isolated RNA underwent quantification and QC using a Bioanalyzer Eukaryotic Total RNA Nano Assay (Agilent). Libraries were constructed using the KAPA mRNA HyperPrep kit (Roche) and NEXTFLEX Unique Dual Index Barcodes (PerkinElmer). Libraries were pooled and sequenced on a NovaSeq 6000 S4 flow cell (Illumina) for 150 bp paired end reads resulting in approximately 25 million uniquely mapped reads per sample.