GSM9316576: PLCPRF5-siCOPG1_2, Day 2; Homo sapiens; RNA-Seq
Cells were kept observing after siRNA transfection to check for the cell status, and 48 hours later, cells were harvested using Rneasy mini kit (Qiagen). 3ug of total RNA was used for the construction of the seuencing libraries. RNA libraries were prepared for RNA sequencing based on the manusfactor's instructions. The adapter contamination were trimmed by Cutadapt and the trimmed read were further processed by Trimomatic to filter out low quality reads.