R62 (CRX1566989; CRA023929)
Eukaryotic mRNA was enriched with magnetic beads with Oligo (dT). mRNA was randomly interrupted by adding Fragmentation Buffer. The first cDNA strand was synthesised using mRNA as a template with six-base random hexamers, and then the second cDNA strand was synthesised with the addition of buffer, dNTPs, RNase H and DNA polymerase I. The cDNA was purified using AMPure XP beads. The purified double-stranded cDNA was then subjected to end repair, addition of the A tail and ligated with sequencing junctions, and then fragment size selection was performed with AMPure XP beads. Finally, the cDNA library was enriched by PCR. submitted_instrument_model: Illumina NovaSeq 5000