GSM9078285: Ma APOEhi.(1); Homo sapiens; RNA-Seq
miRNeasy Mini Kit (Qiagen #217084) The concentration and integrity of total mRNA were assessed using the Agilent 2100 Bioanalyzer and the RNA 6000 Nano Kit (Agilent Technologies). High-throughput RNA sequencing (mRNA-Seq) was performed by Genomics BioSci & Tech Co. (New Taipei City, Taiwan) following the provider's standard protocol. In brief, 1 µg of total mRNA was used for library construction using the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA). The prepared libraries were sequenced on the Illumina NovaSeq 6000 platform with 150 bp paired-end reads.