GSM8996102: 772_PXR_WT_100mgPCN; Mus musculus; RNA-Seq
Total RNA was isolated from the mice's liver tissues using a hybrid RNAzol RT Kit (Sigma Aldrich, St. Louis, MO, USA) according to the provided instructions. The isolated total liver RNA was DNase treated with RNase-Free DNase set (Qiagen) coupled with RNase MiniElute Cleanup kit (Qiagen). The quality of the samples was ensured using Agilent Bioanalyzer 2100 or Advanced Analytical Fragment Analyzer. The concentration of the samples was measured with Qubit®/Quant-IT® Fluorometric Quantification, Life Technologies, and/or Nanodrop ND-2000, Thermo Scientific. The library preparation was performed according to the library preparation protocol (Illumina Stranded Total RNA Reference Guide, Illumina (1000000124514)