GSM9110410: hicut, CEBPA_4h_rep4; Homo sapiens; OTHER
Cells were fixed with 1% formaldehyde in RTfor 10min and then suspended with Hi-C lysis buffer on ice for 30min. DNA was then digested with DdeI and DpnII enzyme,. End-filling master mix was used to fill in restriction fragment overhangss and ligation master mix was used to ligate the DNA fragments.   Add the proximity ligated nuclei in Wash Buffer to activated beads abd then treated with primary CEBPA Rabbit mAb and Secondary Anti-Rabbit.  Chromatin was incubated pA/G-Tn5 transposase then DNA for tagmentation and extracted using DNA Extract Beads Pro. After PCR amplification(14 cycles), size selection was performed using Ampure XP beads.