GSM8726459: BRAFiR 888mel, 0h, biological replicate 1; Homo sapiens; RNA-Seq
RNA was extracted using the RNeasy Plus Mini kit (Qiagen) and quantified with Qubit RNA Broad Range kit and the Qubit 4 (Invitrogen). RNA quality was assessed using the RNA 6000 Nano kit and the 2100 Bioanalyzer (Agilent Technologies). Library was prepared with TruSeq Stranded mRNA kit (Illumina). Samples were indexed and analyzed by paired-end sequencing of 75 base pairs on the HiSeq 2500 instrument (Illumina).