RNA-Seq of Homo sapiens: curcumin treatment after empty vector transfection in EV-D68-infected RD cells
RD cells were transfected with vector and treated with curcumin after EV-D68 infection. TTotal RNA was extracted from RD cells using TRIzol reagent according to the manufacturer's instructions. RNA integrity was assessed with an Agilent Bioanalyzer. Polyadenylated RNA [poly(A)+ RNA] was selectively enriched using oligo(dT) magnetic beads (library selection: other), then fragmented and reverse transcribed to cDNA. Libraries were prepared using the NEBNext Ultra RNA Library Prep Kit and sequenced on the Illumina HiSeq 4000 platform to generate 150 bp paired-end reads. Sequencing was performed by Shanghai Majorbio Bio-Pharm Technology Co., Ltd.