GSM9684774: MOLT4,Untreated; Homo sapiens; RNA-Seq
The MOLT-4 cell line was cultured under standard conditions in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin at 37 °C in a humidified incubator with 5% CO₂. The cell suspension was collected and centrifuged at 300 × g for 5 minutes at 4 °C. The supernatant was discarded, and the cell pellet was washed twice with sterile PBS containing 0.04% BSA to remove residual media and serum components. Following washing, cells were resuspended gently to obtain a single-cell suspension. Cell clumps were removed by passing the suspension through a 40–70 µm cell strainer. Cell viability and concentration were determined using Trypan Blue staining, ensuring viability >85%. Finally, the cells were resuspended in PBS containing 0.04% BSA at a concentration of 700–1200 cells/µL and kept on ice prior to loading onto the 10x Genomics platform for downstream single-cell RNA sequencing.