Whole-genome sequencing of native Phragmites australis leaf (Desert ecotype)
Genomic DNA was extracted from leaf tissue of a greenhouse-grown wild-type Phragmites australis seedling originating from a native desert population in Abu Dhabi, UAE. DNA was isolated using the NucleoSpin Plant DNA Isolation Kit. Libraries were prepared by random fragmentation using Covaris, followed by end repair, A-tailing, and adapter ligation. Circularized DNA was amplified using Rolling Circle Replication, and sequencing was performed using the DNA Nanoball (DNB) technology on the BGISEQ platform with PE150 reads.