Shotgun metagenomics sequencing of high-pressure bioreactor R2 at 40 bar
Shotgun metagenomic library prepared using Celero DNA-Seq library prep kit (Tecan) following manufacturer protocols. Input DNA and libraries quantified with Qubit 2.0 Fluorometer. Quality assessed with Agilent 2100 Bioanalyzer High Sensitivity DNA assay. Paired-end 300 bp sequencing on Element AVITI platform using avidity sequencing chemistry.