GSM9380759: MDC_P4_H1, untreated, time point D40; Homo sapiens; RNA-Seq
Total RNA was isolated from  on differentiation days 15, 25, and 40 using TRIzol™ Reagent (Thermo Fisher Scientific). Following DNase digestion with Promega RQ1 RNase-Free DNase (M6101), RNA was purified using a phenol-chloroform extraction protocol, and RNA concentration was measured with a NanoDrop spectrophotometer. Sequencing libraries were prepared according to the NEBNext Ultra II RNA Poly(A) Workflow which included the NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490), NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760L), NEBNext Sample Purification Beads, and NEBNext Multiplex Oligos for Illumina (Set 4, E6443). Each library was generated with an input of 400 ng total RNA per sample. Paired-end RNA libraries were sequenced on the NovaSeq X Plus platform (Illumina) in PE100 mode with a loading concentration of 200 pM. Sequencing was conducted by the Core Facility Genomics at the Berlin Institute of Health (BIH).