16s Seq of mus musculus: adult feces
The V3V4 hypervariable regions of the bacterial 16S rRNA gene were amplified by PCR using region-specific primers with Illumina sequencing adapters. PCR products were purified, quantified, and pooled in equimolar concentrations. Sequencing libraries were constructed following standard Illumina protocols and sequenced on an Illumina platform using paired-end 250 bp reads