GSM8464464: X11-Tight-/-; Mus musculus; RNA-Seq
Take an appropriate amount of quadriceps muscle into the grinding tube, add TRIZOL, and put it into the tissue grinder to crack the histiocytic components.Then centrifuge, transfer the supernatant to a new EP tube, add chloroform, after full shock, let stand for 10min, and centrifuge again.The supernatant was transferred to a new EP tube, isopropyl alcohol was added to assist precipitation, the supernatant was abandoned, 75% anhydrous ethanol was washed and precipitated twice, and finally the total RNA was dissolved and precipitated with enzyme-free water.The total RNA was reverse-transcribed into cDNA. The RNA-seq libraries were sequenced on a BGIseq500 platform to generate paird-end 150 bases reads