Illumina MiSeq of fungal communities in mediterrean urban and peri-urban landscapes
Libraries were prepared using the Illumina two-step amplicon protocol. PCR1 products were purified, indexed in a second PCR, purified again, quantified, pooled at equimolar concentrations, and sequenced on an Illumina MiSeq platform using the MiSeq Reagent Kit v2 (500 cycles) for paired-end sequencing.