GSM8726047: Bridge-Multi, RNA; Homo sapiens; RNA-Seq
K562 cells were centrifuged in growth media at 300 x g for 5 mins. Adherent cells (HEK and iPS) were dissociated with accutase, resuspended in PBS and centrifuged at 300 x g for 5 mins. Organoids (Brain and Retinal) were dissociated with a papain-based solution, cryopreserved, thawed and centrifuged at 300 x g. Single cells were encapsulated in GEMs using the 10X chromium controller with the associated kit (scATAC v1.1 for Phospho-seq, scATAC+RNA for Phospho-seq-multi, and scRNA v3.1 for Organoid RNA-seq scATAC-seq, scRNA-seq libraries were constructed according to the associated kits methods from 10X genomics. ADT and HTO libraries were constructed according to the ASAP-seq methods (Mimitou et al, 2021) and Phospho-seq methods (https://phospho-seq.com/post/)