RNA-seq of PDOs treated with AMG510
RNA-seq of PDOs treated with AMG510
Patient derived organoids were treated with 100 nM AMG510 for 24 h. Total RNA was extracted using an RNeasy mini kit (Qiagen) and quantified using 2100 Expert Bioanalyzer software (Agilent). RNA-seq analysis was outsourced to Macrogen Japan Corp. (Tokyo, Japan). Following the manufacturerâ€™s instructions, total RNA with an RIN>7 was used to generate a barcode-labeled library using an Illumina TruSeq Stranded mRNA LT Sample Prep Kit (Illumina). 