RNAseq of Tetraselmis sp. NKG400013: day1 rep2
RNA-seq libraries were prepared using the SMART-Seq mRNA Kit (Takara Bio) according to the manufacturer's protocol. Briefly, first-strand cDNA was synthesized using the SMART method, amplified by PCR, and purified with AMPure XP beads. Sequencing libraries were then constructed using the Nextera XT DNA Library Prep Kit (Illumina), in which the amplified double-stranded cDNA was fragmented and adapter-tagged by transposon-mediated tagmentation, followed by PCR amplification using indexed primers from IDT for Illumina DNA/RNA UD Indexes. Library quality was assessed by electrophoresis. The libraries were sequenced on an Illumina NovaSeq 6000 with 150-bp paired-end reads. Raw BCL files were converted to demultiplexed FASTQ files using BCL Convert v4.3.6.