GSM9436174: Embryos treated with A-485 Replicate 3; Ambystoma mexicanum; RNA-Seq
RNA was isolated using TRIzol (Invitrogen) and syringe needles for initial tissue disruption, and then following instructions included with a Monarch Total RNA Isolation Kit (NEB). RNA samples were submitted to a commercial vendor (Novogene) to generate approximately 25-30 million raw reads per sample on an Illumina sequencer.