GSM8724833: mBMSC_25+27; Mus musculus; RNA-Seq
RNA integrity was assessed using a Fragment Analyzer system with the RNA Fragment Kit (Agilent Technologies). All samples had an RNA quality number (RQN) greater than 7. Sequencing libraries were prepared from 30 ng of total RNA using the QuantSeq 3´ mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the manufacturer's protocol. The libraries were quantified with the Qubit dsDNA assay kit and Qubit 2 fluorometer (Thermo Fisher Scientific), and their quality was evaluated using a Fragment Analyzer system with the NGS Fragment Kit (Agilent Technologies). Sequencing was performed on a NovaSeq X Plus Sequencing System using the NovaSeq™ X Series 25B Reagent Kit (300 Cyc) using one lane in 2 × 157 bp mode, generating an average of 120 million reads per sample. Only read1 were used for analysis.