16S-Seq of larval food from Melipona quadrifasciata
DNA was extracted from larval food of stingless bee using the Wizard Magnetic DNA Purification System for Food (Promega), following the manufacturers protocol and DNA quality and quantity were assessed via NanoDrop spectrophotometry. The V3V4 region of the 16S rRNA gene was amplified using primers 341F and 805R with Illumina overhangs, following the Illumina 16S Metagenomic Sequencing Library Preparation protocol. Amplicons were purified with AMPure XP beads, quantified with the Quantus kit, and pooled at 10ng/L. Sequencing was conducted on an Illumina NextSeq 1000 using the P1-600 cycle kit, generating 2  300bp paired-end reads.