GSM9710205: Control iPSC-derived neuron rep 1; Homo sapiens; RNA-Seq
Total RNA was isolated from mature human iPSC-derived neurons using the RNeasy Kit according to the manufacturer's instructions. RNA concentration was measured using a Qubit fluorometer, and RNA integrity was assessed using a Bioanalyzer. RNA-seq libraries were prepared from 500 ng total RNA using the TruSeq Stranded mRNA Library Preparation Kit. Library molarity and quality were assessed using a Qubit fluorometer and TapeStation DNA High Sensitivity chip. Libraries were sequenced on an Illumina HiSeq 4000 platform using TruSeq SBS chemistry to generate single-end 100-bp reads.