GSM9315906: ribo_ded1-ts replicate 2_37 deg; Saccharomyces cerevisiae; RNA-Seq
Cells were rapidly vacuum filtered and flash frozen. Whole cell extracts were prepared by breaking cells in freezer mill. Ribosome profiling (Rnase I footprinting, size selection,RNA extraction, preadenylated linker ligation, reverse transcription, cDNA circularization, PCR amplification) was done as described  Sen et al 2019