RNA-Seq of maize leaf
Total RNA was extracted from leaves of maize (Zea mays L.) inbred line 'B73' subjected to three different treatment conditions: no aphid infestation, Rhopalosiphum padi infestation, and Rhopalosiphum maidis infestation (each with four biological replicates). mRNA was enriched from total RNA using Oligo-dT magnetic beads. Following fragmentation, first-strand cDNA was synthesized using random hexamer primers, followed by second-strand synthesis. The double-stranded cDNA underwent end repair, A-tailing, adapter ligation, size selection, PCR amplification, and purification to generate the final sequencing libraries. The qualified libraries were pooled and sequenced on an Illumina platform to generate paired-end reads.