spatial transcriptomic libraries from Stereo-seq v1.3 (211ST13114-CG, Complete Genomics); The libraries are constructed by capturing poly-adenylated RNA onto a silicon chip patterned with DNA Nanoballs (DNBs) and indexed by Stereo-seq 16 Barcode Library Preparation Kit (111KL160-CG)
Stereo-seq v1.3 libraries are constructed by capturing poly-adenylated RNA onto a silicon chip patterned with DNA Nanoballs (DNBs) that contain random Coordinate Identifiers (CIDs) and Molecular Identifiers (MIDs). Following in situ reverse transcription to link transcripts to their spatial coordinates, the resulting cDNA is pooled, amplified, and fragmented to prepare circularized libraries. These libraries are then sequenced using DNBSEQ technology, where rolling circle amplification creates high-density DNA Nanoballs that are loaded into patterned flow cells for high-accuracy, low-index-hopping imaging on a DNBSEQ-T7 sequencer.