ON of Parus major: adult female, 1A inversion
DNA was isolated from the fibroblast cell culture derived from female great tits heterozygous with the rearrangement in 1A chromosome using the standard phenol-chloroform method. The library was prepared using the recommended protocol for LSK114 kit from the manufacturer (Oxford Nanopore, UK). Sequencing was performed on a MinION device with the FLO-MIN114 Flow Cell (Oxford Nanopore, UK), yielding an approximately 10x genome-wide coverage.