Genome sequencing of Mycobacterium sp.
DNA libraries are prepared with Oxford Nanopore Technologies (ONT) SQK-RBK114.96 kit (ONT, United Kingdom) using 200-400 ng of High Molecular weight (HMW) DNA. Barcoded samples are pooled together into a single sequencing library and loaded in a FLO-MIN114 (R.10.4.1) flow cell in a GridION (ONT, United Kingdom).