16S rRNA V3-V4 archaeal amplicon sequencing of high-pressure bioreactor R1 at 40 bar, replicate c
Archaeal 16S rRNA V3-V4 hypervariable region amplified using archaeal-specific primers Arch340wF (5'-CCCTAYGGGGYGCASCAG-3') and Arch806R (5'-GGACTACVSGGGTATCTAAT-3'). Library prepared following Illumina 16S Metagenomic Sequencing Library Preparation protocol with two PCR amplification steps (35 cycles initial PCR + index PCR). Paired-end 300 bp sequencing on Element AVITI platform using avidity chemistry.