GSM8488395: Acute preterm injury 1; Homo sapiens; RNA-Seq
Human lung tissue from infants who died with BPD, BPD+PH, and term infant controls were digested in an enzymatic cocktail [collagenase I/dispase II (1 μg/ml) tissue or Miltenyi Multi-Tissue Dissociation Kit] using a gentleMACS Octo Dissociator. Tissue lysates were serially filtered through sterile gauze, 100- and 40-μm sterile filters (Fischer). Single-cell suspensions then underwent cell sorting using serial columns (Miltenyi Microbeads, CD235a and CD45). CD45− and C45+ populations were mixed 2:1 and were used as input for generation of scRNA-seq libraries using the 10x Chromium platform 3' or 5′ library preparation kits (10x Genomics) following the manufacturer's recommendations and targeting 10,000-20,000 cells per sample.