Nanopore RNA-seq of Ceratitis capitata single early embryos
Medfly embryos were collected but the timing is unknown. Suffice is to say that these are early embryos before hatching. The embryos were put in RNASafeguard reagent. Total DNA and RNA were extracted from each embryo, ERCC spiked in, and cDNA libraries generated which were sequenced with Nanopore technology.