RNA-seq of Vigna radiata
Total RNA was extracted using TRIzol, mRNA enriched by oligo-dT beads, fragmented and reverse-transcribed to double-stranded cDNA. After end-repair, A-tailing and adapter ligation, 250-300 bp fragments were selected by AMPure beads and PCR-enriched for 12 cycles. Library quality was assessed on Agilent 2100 Bioanalyzer