CONTORL
To construct the sequencing library, DNA or RNA was first extracted from the target samples. For DNA sequencing, genomic DNA was extracted from environmental samples using standard DNA extraction kits (e.g., FastDNA SPIN Kit for Soil). For RNA sequencing, total RNA was extracted using RNA extraction kits (e.g., RNeasy MinElute Cleanup Kit) and reverse-transcribed into cDNA. The extracted DNA or cDNA was subjected to quality control (e.g., agarose gel electrophoresis and spectrophotometry) before library preparation using Illumina-compatible kits (e.g., Nextera XT DNA Library Prep Kit). The library preparation steps included DNA fragmentation, end repair, adapter ligation, and PCR amplification. After library construction, quantification methods (e.g., qPCR or Bioanalyzer) were used to assess library concentration and fragment size to ensure they met sequencing requirements. Finally, the libraries were sequenced on an Illumina platform (e.g., NovaSeq 6000) for high-throughput sequencing.