GSM9107482: Vehicle-treated, HSV-1 2h (MOI5), Input control replicate2; Homo sapiens; ChIP-Seq
Isolated nuclei were lysed, and samples were sonicated using a Covaris M220 Focused-Ultrasonicator (8 min, 10% duty cycle, 75 W incident power, 200 burst per cycle) to obtain DNA fragments of 200-500 base pairs (bp). Chromatin samples (350-450 µg) were precleared with Protein G Dynabeads (ThermoFisher) for 1 h at 4°C followed by incubation with 3-5 µg antibodies for 14 h at 4°C. Chromatin samples were incubated with 1:1000 dilution of anti-RNAPII-NTD (Cell Signaling 14958) Libraries were sequenced on a NovaSeq X Plus 1.5B to yield 3.10 - 3.60 x 108 paired-end reads per sample.