Blastocyst 2
This kit relies on three key technologies to deliver high-diversity whole genome libraries from single cells. After cell lysis, a high-fidelity MDA reaction is used to amplify microgram amounts of gDNA using the high-fidelity REPLI-g DNA Polymerase. The amplified gDNA is then fragmented into short inserts using QIAGENs highly random enzymatic FX DNA fragmentation reaction. The fragmented DNA is then end-repaired and an A base is added to the 3 ends to prepare the insert for ligation. Lastly, a ligation reaction adds the unique dual index to both ends of the insert. The entire process, beginning with an isolated single cell, is PCR-free and requires only 3.5 hours to complete