Multiplexed amplicon sequencing of environmental microbiomes: Delaware
PCR amplicons for rRNA (16S and 18S) and functional (arrA, dsrB, mcrA, narG, nirK, nrfA, pmoA, phoD, and phoX) were amplified with a common 5'-head sequence on all primers. This enabled barcoding in a second PCR. The 11 barcoded amplicons from 25 samples were mixed and sequenced on a single MiSeq run.