Microglial colonization is shaped by intrinsic and extrinsic CSF-1 during early forebrain development
Microglial colonization is shaped by intrinsic and extrinsic CSF-1 during early forebrain development
CD45+ cells were enriched using CD45 microbeads from Miltenyi (Cat# 130-052-301) and the Automacs using possel function.  cells were captured and processed in a two run. Run 1 = Sample 1 ; Run 2 = Sample 2. Four sample tags were used in Run 1 (comprising 2 mix, and 2 wild type), pooled using the BDTM Ms Single Cell Sample Multiplexing Kit (Cat No: 633793). Six sample tags were used in Run 2 (comprising 1 mix, 2 wild type and 3 EMX1creCSF1flox) The sample was processed according to the BD Rhapsodyâ„¢ Whole Transcriptom protocol