iEFC-EM_day8-Replicate1 (CRX1826036; CRA016958)
For iEFCs, cells were dissociated with accutase. For ciMOs, ciMOs were digested with 100 uL 10 U/ml papain (Worthington Biochemicals, LS003126) at 37 deg C for 15-30 min. Single cells were washed twice with cold PBS, filtered through a 35 um cell strainer, and loaded onto 10x Genomics Chromium system using Single Cell 3' Reagent Kits V3.1 at a concentration of 16000 single cells per sample.