GSM9109819: Plate1_TF_FOXO1_P1D11_dose0.05_rep2; Homo sapiens; RNA-Seq
At 48 hours post transfection, we removed the media, added 32uL Trizol to the cells in each well to extract RNA, mixed thoroughly by pipetting, snap froze in dry ice + EtOH and stored the plate at -80C until ready for RNA purification. After thawing the Trizol prepped cells on ice, we used the Zymo Direct-zol-96 RNA kits (Zymo #R2056) to purify RNA. For RNA library preparation, we sent the total RNA to Novogene for bulk unstranded mRNA library preparation with PolyA enrichment and sequencing on an Illumina NovaSeq X Plus (paired end 150 cycles).