GSM8726273: 8_m6A-seq_SNU2491_DMSO; Homo sapiens; OTHER
From 2-5 million cells, total RNA was extracted using the QIAzol reagent following the manufacturer's instructions. After extraction, 200 ng of total RNA was used to perform ULI-MeRIP using 1 µg of anti-m6A antibody (Cat#E1610S, New England Biolabs) as previously described. The m6A-modified RNA was immunoprecipitated and used as an input for library construction using the SMARTer® Stranded Total RNA-Seq Kit v2 (Cat#634411, Takara) according to the manufacturer's protocol.