GSM9107795: primary mouse chondrocytes from Tiki2 fl/fl mice, rep2; Mus musculus; RNA-Seq
Total RNA was extracted from cells using TRIzol reagent and purified using the RNeasy Mini Kit (Qiagen). RNA integrity was verified using the Agilent 4200 TapeStation. Poly(A) mRNA was enriched with oligo(dT) beads, fragmented, and reverse-transcribed with random primers and M-MuLV. After second-strand synthesis (RNase H / DNA Pol I), ds-cDNA was end-repaired, A-tailed, ligated to Illumina adapters, size-selected (~200 bp) with AMPure XP beads, PCR-amplified, and purified to yield the final RNA-seq library.