RNASeq from colonic tissue of naïve IEC-Bmal1-/- mice and Bmal1flox controls over the course of a day
RNASeq from colonic tissue of naïve IEC-Bmal1-/- mice and Bmal1flox controls over the course of a day
Colon tissue was harvested and snap frozen in liquid nitrogen. Colon tissue was homogenised in lysing MatrixD tubes (MP biomedical) containing TRIzol (Invitrogen), using a bead mill homogeniser (Fisherbrand). RNA was extracted with chloroform and aqueous phase was precipitated with isopropyl alcohol. Supernatant was washed with 70% ethanol and the RNA pellet was resuspended in nuclease-free water. RNA libraries were prepared using standard Illumina protocols with TruSeq Stranded mRNA assay (Illumina)