metabarcoding of seawater eDNA: MiFish-U targeting 12S for Actinopterygii
eDNA extracted with QIAGEN Dneasy Blood and Tissue kit, PCR performed with MiFish-U primers, size-selection with SeraMagBead purification, 2nd PCR library preparation with Illumina TruSeq indices, sequencing on MiSeq with v3 600 kit