GSM9791036: RNAseq_High_2; Mus musculus; RNA-Seq
RNA extraction using the Qiagen RNeasy kit. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing,adapter ligation, size selection, amplification, and purification.