GSM9743349: Cx3Cr1CreCD31fl/+5xFAD mice_2; Mus musculus; RNA-Seq
Brain tissues excluding cerebellum were rapidly dissected and processed for nuclei isolation. Isolated nuclei were purified and filtered to remove debris and aggregates before downstream single-nucleus RNA sequencing library preparation. Single-nucleus RNA-seq libraries were generated using the Chromium Single Cell 3′ Gene Expression platform (10x Genomics) according to the manufacturer's instructions. Briefly, isolated nuclei were loaded into the Chromium Controller to generate gel bead-in-emulsions (GEMs). Barcoded cDNA was synthesized by reverse transcription, followed by amplification, fragmentation, adaptor ligation, and sample index PCR. Final libraries were sequenced on an Illumina platform using paired-end sequencing mode.