GSM8578981: 136401_m8neg, MPHOSPH8-deficient cerebral organoid day27, scRNAseq; Homo sapiens; RNA-Seq
Single-cell suspensions from two cerebral organoids per condition were prepared at day 27 using 0.9×:1× Accutase/Trypsin (Gibco, 11538876) and incubated for 15 min on 37°C while shaking and dissociating manually by pipetting every 5 min. Digestion was stopped by gradually adding two volumes of cold DMEM/F-12, washed with DPBS−/− (Thermo Fisher Scientific, 14190094) plus 0.04% BSA (VWR, 9048-46-8). Cells were then filtered through a cell strainer, counted, and loaded onto a 10x Genomics controller in DPBS−/− plus 0.04% BSA according to the manufacturer's protocol with a target cell number of 4000 cells. Library was performed according to the manufacturer's instruction (Single Cell 3' v3, 10X Genomics)