GSM9263491: Knockout Cajal-Retzius cells, postnatal day 14, scRNAseq; Mus musculus; RNA-Seq
The cell suspension was prepared using the Papain Dissociation System (Worthingon Biochemical Corporation). The resulting cell pellet was resuspended in oxygenated aCSF and DAPI was added for live/dead cell discrimination. Cells were then sorted using a SONY MA900 cell sorter. Sorted DAPI- tdT+ cells suspended in aCSF were used to generate the gel-beads cell emulsion by the 10X Chromium Controller (PN-1000202). Gene expression libraries were prepared using the Chromium Next GEM Single Cell 3' Gene Expression v3.1 (Dual Index) (PN-1000269) following the manufacturer's protocol. Sample libraries were sequenced in Illumina NovaSeq PE150 to an average depth of ~ 50,000 reads per cell.