GSM8796361: SMARCA5 KOBMDM, 2h LPS 10ng/ml, replicate4 (RNA-seq); Mus musculus; RNA-Seq
Total RNA was extracted using the Zymo Quick-RNA Miniprep Kit (Zymo Research, Cat# ZYR1055) following the manufacturer's instructions. RNA quality was assessed using the High Sensitivity RNA ScreenTape Assay (Agilent, Cat# 5067-5579) on the Agilent TapeStation system, and only samples with RIN > 9 were used for library preparation. Stranded RNA-seq libraries were prepared using the Illumina TruSeq Stranded Total RNA Library Prep Gold Kit (Illumina, Cat# 20020598) with 300 ng of high-quality RNA per sample. Library size distribution was confirmed using the Agilent TapeStation D5000 ScreenTape Assay (Agilent, Cat# 5067-5592), with an average fragment size of ~300 bp. Libraries were quantified with the QuantiFluor dsDNA System (Promega, Cat# E2670), normalized to 4 nM, pooled, and sequenced as 51-bp paired-end reads on an Illumina NovaSeq 6000 platform.