RNA-Seq of danio rerio
Total RNA was extracted from the biological samples using standard phenol-chloroform or column-based purification methods, followed by quality assessment using capillary electrophoresis. RNA-seq libraries were prepared from high-quality RNA using a poly(A) selection or rRNA depletion strategy, depending on sample type, followed by cDNA synthesis, fragmentation, end-repair, adapter ligation, and PCR amplification according to Illumina-compatible library preparation protocols. Libraries were quantified and pooled in equimolar amounts prior to sequencing.Sequencing was performed on an Illumina platform, most likely the NovaSeq 6000 system, generating paired-end reads for high-throughput transcriptome profiling. Raw sequencing data (FASTQ files) were demultiplexed and subjected to standard quality control procedures, including adapter trimming and filtering of low-quality reads. The resulting clean reads were used for downstream RNA-seq analysis.