GSM8515971: dual-V2, 5E+10, replicate2; Mus musculus; OTHER
Genomic DNA was extracted from tissues using TIANamp Genomic DNA Kit (DP304, Tiangen, China) The sgRNA-targeted loci were amplified using Taq PCR MasterMix (KT211, Tiangen, China) and purified by TIANgel Purification Kit (DP219, TIANGEN).