Effects of warming and reduced rainfall on the abundances and composition of soil fungal communities across seasons
To identify the fungi present in the ingrowth bags across treatments and seasons, total genomic DNA was extracted using Qiagen DNEasy PowerSoil kits. The ITS2 rDNA subunit was PCR amplified using the dual-index barcoding approach described in Gohl et al., (2016). The fungal primer set targeting the ITS2 region was forward primer, 5.8SR (TCGATGAAGAACGCAGCG) and reverse primer, ITS4 (TCCTCCGCTTATTGATATGC). For PCR, 25 cycles were used for amplicon generation. Amplified products were magnetically cleaned using the Agencourt AMPure XP kit and quantified using a Qubit dsDNA HS Fluorometer. Each of the amplified samples were pooled at equimolar concentration into a single library and sequenced at the University of Minnesota Genomics Center using the Illumina NextSeq 2000 platform (2  300 bp).