GSM9710014: MV4-11 cells ectopically expressing DNMT1 10KR, gDNMT1 and gNTC CRISPR knockout, DMSO for 7 days, rep 1; Homo sapiens; OTHER
gDNA was extracted using NEB's Monarch® Spin gDNA Extraction Kit as per vendors instructions. NEBNext® Enzymatic Methyl-Seq (E7120S) was carried out was per vendors instruction. Genomic DNA shearing was performed using optimised conditions for NEBNext UltraShear® (M7634S). Sheared DNA was purified using 0.8x AMPure XP reagent (Beckman Coulter) and 50ng total genomic DNA was subject to the EM-seq kit.