JSJ-15-3.mRNA.fastq (CRX1828957; CRA027529)
Total RNA was isolated from soil using the Qiagen RNeasy FFPE Kit with on-column DNase I digestion. RNA quality was assessed using Agilent TapeStation High Sensitivity RNA ScreenTape. Ribosomal RNA (rRNA) was depleted using Illumina Ribo-Zero Plus rRNA Depletion Kit. Strand-specific RNA-seq libraries were then prepared from the rRNA-depleted RNA using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760) according to the manufacturer's instructions.