DAP-Seq of Oryza sativa Japonica Group: GPC6 DAP in endosperm replicate 1-1
DAP DNA was extracted, then enriched DNA fragments were sheared by ultrasonication. The fragmented DNA was end-repaired, A-tailed, and ligated with Illumina sequencing adapters. DNA fragments with an insert size of 100300 bp (including adapter sequences) were selected and PCR amplified. The qualified libraries were sequenced on an Illumina NovaSeq 6000 platform by Gene Denovo Biotechnology Co. (Guangzhou, China).