RNA-Seq of C. elegans: fem-3; nhr-49 Day 1 adult feminized, replicate 3
For RNA-Seq libraries: Total RNA was extracted from Day 1 adult C. elegans using Trizol reagent. RNA quality was assessed via Bioanalyzer, and 500 ng of input RNA was used for library preparation with the Lexogen QuantSeq 3 mRNA-Seq Library Prep Kit (FWD). Libraries were sequenced on an Illumina platform with single-end reads.