E3m_Epi_mrna
Total RNA samples from 72-hour cultures in YPD agar were prepared, maintaining FK506 drug pressure in resistant isolates. RNA was isolated with QIAGEN miRNeasy Mini Kit and divided into small and long RNA preparations. rRNA-depleted RNA libraries (long RNA) were prepared using Illumina Stranded Total RNA Prep and Ribo-Zero Plus rRNA Depletion Kit using M. circinelloides rDNA-specific probes, and 150-bp paired-end reads were obtained with NovaSeq X Plus sequencing system.