PCR
Sequencing of target capture data from genomic DNA from tissue, library preparation through enzymatic shearing with the NEBnext Ultra II FS kit, targeting 300bp insert size, capture reactions using MyBaits-II kit (Arbor Biosciences, MI, USA; Ref# 170210-32) and protocol described in Wielstra et al. 'Phylogenomics of the adaptive radiation of Triturus newts supports gradual ecological niche expansion towards an incrementally aquatic lifestyle' (2019) - https://doi.org/10.1016/j.ympev.2018.12.032.