MST6 (CRX773781; CRA012359)
Spatial transcriptome sequencing (10X Genomics, Visium) were performed on 5 mouse esophageal tissues, i.e., NOR (taken at week 0), INF (taken at week 14), LGIN (taken at week 18), HGIN (taken at week 24), and mESCC (taken at week 26), with high RNA quality. The frozen samples were embedded in Optimal Cutting Temperature compound (OCT, Sakura Tissue-TEK) on dry ice, with NOR/INF in one cryomold and LGIN/HGIN/ESCC in the other. The OCT blocks were cut at 10 microm thickness with a pre-cooled cryostat and transferred onto the 6.5 mm X 6.5 mm oligo-barcoded capture areas of the Visium Spatial Gene Expression slides. Sequence libraries were sequenced at recommended depth using Illumina NovaSeq 6000 platform. Raw sequencing data was mapped to the pre-built mm10 reference genome using the Spaceranger workflow (version 1.0.0).