GSM9262896: WT38-LHG23987; Homo sapiens; ATAC-seq
Tissues were thawed on ice and weighted prior to RNA extraction. The samples were minced and homogenized using a glass dounce homogenizer. Total RNA was extracted using the MiniBEST Universal RNA Extraction Kit (TaKaRa, #9767). For cells, total RNA was extracted using the MiniBEST Universal RNA Extraction Kit (TaKaRa, #9767); ATAC-seq was performed on 50,000 cells following a standard protocol with minor modifications. After lysis and washing, nuclei were tagmented with Tn5 transposase; The CUT&RUN assay was performed with 5 million cells, nuclei were isolated and incubated overnight at 4°C with FLAG or H2A antibody, followed by pA-MNase binding, chromatin digestion, and DNA purification. Library preparation and sequencing were performed by Azenta Life Sciences company.