GSM9436767: SW620-shNC2; Homo sapiens; RNA-Seq
Total RNA was extracted from SW620-shECM1 and SW620-shNC cells using a commercial RNA extraction kit (Trizol reagent or RNeasy Mini Kit) following the manufacturer's instructions. RNA quality and integrity were assessed using Nanodrop and Agilent 2100 Bioanalyzer, with RIN (RNA Integrity Number) ≥ 7.0 considered acceptable for subsequent experiments. Single-cell RNA sequencing libraries were constructed using a single-cell RNA library preparation kit (10x Genomics Chromium Single Cell 3' Reagent Kit). Briefly, viable cells were sorted, and single-cell suspensions were prepared; then, cell barcoding and cDNA synthesis were performed via reverse transcription. After cDNA amplification, library fragmentation, adapter ligation, and PCR enrichment were conducted. The final libraries were quantified using Qubit and validated by Agilent 2100 Bioanalyzer for size distribution (typically 200-500 bp).