GSM8464625: Jurkat, WCE; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 15 min at RT with rotation. After quenching and cell lysis, chromatin was sonicated using a Covaris S220 Sonicator ChIP-seq libraries were prepared using the End-It DNA End-Repair Kit (Lucigen), Klenow enzyme (3'-5' exo 5U/ul, NEB) for A-tailing and Quick Ligation Kit (NEB) for adapter ligation. Libraries were amplified (PFU Ultra II HS (Agilent)) using 14 cycles on the thermocycler. Post amplification libraries were purified using Agencourt AMPure XP beads (Beckman Coulter) and validated using the Agilent High Sensitivity DNA kit.