PDA.TGS (CRX2094867)
For the construction of the PacBio SMRT bell library, the PacBio RSIII technology was used, with an insert size of approximately 18 kb. Subsequently, sequencing was performed on the PacBio Sequel IIe platform. The raw fastq data were filtered using fastp v0.23.4 to remove adapters and low-quality reads, resulting in clean sequences. Prior to genome assembly, Jellyfish (version 2.3.0) and GenomeScope (version 2.0) were employed to assess the genome DNA size and heterozygosity, with a kmer size of 21 bp. Afterwards, ABySS (version 2.1.5) was used to assemble short reads into scaffolds, and BlasR was utilized to align these scaffolds with PacBio sequencing results for sequencing data error correction. Finally, the subsequent assembly was completed using CANU v2.2 with default parameters. were employed to assess the genome DNA size and heterozygosity, with a kmer size of 21 bp. Afterwards, ABySS (version 2.1.5) was used to assemble short reads into scaffolds, and BlasR was utilized to align these scaffolds with PacBio sequencing results for sequencing data error correction. Finally, the subsequent assembly was completed using CANU v2.2 with default parameters. The raw fastq data were filtered using fastp v0.23.4 to remove adapters and low-quality reads, resulting in clean sequences. Prior to genome assembly, Jellyfish (version 2.3.0) and GenomeScope (version 2.0) were employed to assess the genome DNA size and heterozygosity, with a kmer size of 21 bp. Afterwards, ABySS (version 2.1.5) was used to assemble short reads into scaffolds, and BlasR was utilized to align these scaffolds with PacBio sequencing results for sequencing data error correction. Finally, the subsequent assembly was completed using CANU v2.2 with default parameters.