GSM8724458: B109,ZEB1LysMDel,24h,M0; Mus musculus; RNA-Seq
For RNA isolation, BMDMs were washed with PBS and lysed with 350 μl RLT Plus from the RNeasy Plus Mini Kit (QIAGEN, 74136) and RNA was isolated following the manufacturer´s instructions Bulk RNA sequencing of BMDM RNA (n=3 per condition) was performed by Novogene using poly(A) enrichment library preparation protocol and paired-end sequencing (PE150).