DNAseq
Total genomic DNA was extracted from using the Mag-Bind Soil DNA Kit (Omega Bio-tek, Norcross, GA, U.S.) per manufacturers instructions. DNA concentration and purity were assessed with TBS-380 and NanoDrop2000, and DNA quality was verified on a 1% agarose gel. DNA extract was fragmented to an average size of about 400 bp using Covaris M220 for paired-end library construction with NEXTFLEX Rapid DNA-Seq (Bioo Scientific, USA). Sequencing was performed on an Illumina NovaSeq 6000 (Illumina Inc., USA) at Majorbio Bio-Pharm Technology (China) using the NovaSeq 6000 S4 Reagent Kit v1.5 (300 cycles).