18S rRNA gene amplicon sequencing of root metagenome: volunteer barley roots growing in standing white mustard cover crop under different tillage practices
Total genomic DNA was extracted using the FastDNA Spin Kit for Soil (MP Biomedicals, California, USA). Amplicon library preparation and sequencing were performed by BMKGENE (Mnster, Germany) using the Illumina NovaSeq platform (Illumina, San Diego, CA, USA).