RNA-Seq T gondii
Ribosomal RNA was removed using the Ribo-Zero rRNA removal kit (human/mouse/rat, Illumina). Sequencing libraries were then generated using the TruSeq RNA Sample Prep Kit (v2, Illumina) according to manufacturers protocol. Libraries were amplified using the TruSeq Cluster Kit (v3, Illumina), and subjected to 50-bp single-end sequencing with the Illumina HiSeq 2000 system.