16S V4 amplicon of soil microcosm: 8 weeks at 25 C  (unlabelled - replicate 1)
The Griffiths method was used to extract DNA from 0.5 g frozen soil. Amplicon sequencing targeting the V4 hypervariable region of the 16S rRNA gene was performed profile bacterial communities. PCR amplification was performed using the modified 515f 5-GTGYCAGCMGCCGCGGTAA-3) and 806r 5- GTGYCAGCMGCCGCGGTAA-3 primer set. Sequencing was performed by Macrogen (Seoul, South Korea) using the Illumina MiSeq platform with paired-end 2 x 250 bp reads. Library preparation, quality control, and sequencing followed Macrogens standardized protocols.