Full-length 16S rRNA amplicon sequencing of high-pressure bioreactor R1 at 40 bar
Full-length 16S rRNA gene (~1500 bp) amplified using universal primers FL16SF (5'-AGAGTTTGATCMTGGCTCAG-3') and FL16SR (5'-CGGTTACCTTGTTACGACTT-3'). PCR conditions: 95C 3 min; 30 cycles of 95C 30s, 55C 30s, 72C 90s; final extension 72C. Library prepared using Oxford Nanopore Ligation Sequencing Kit V14-PCR Barcoding protocol. Sequenced on PromethION platform with Flow Cell R10.4.1.