bulk RNA-Seq of Mus musculus: adult male fungiform taste pappillae
10 m thick coronal sections of fresh frozen tongue from C57BL/6 mice containing FFP were prepared using a cryostat. Sections were stained with hematoxylin& eosin, and pools of ~20-30 from FFP (n=3 pools from 3 mice) were excised using laser micro dissection, and full length bulk RNASeq libraries were prepared using the Ovation RNASeq system V2 (Tecan biosystems, Morgan Hill, CA) per manufacturer instructions.