GSM8689528: m5_CD_Day_0_Male_Progeny; Drosophila melanogaster; RNA-Seq
The samples were thawed and homogenized using the Precellys®24 (Peqlab) system at 5000 RPM for 15 seconds, then centrifuged at 13,000 g for 30 seconds, and the supernatant was pipetted to a new tube to remove residual glass beads. Total RNA was extracted from samples stored at -80 °C in Qiazol using the RNeasy UCP Micro Kit (Qiagen, 73934). Samples were homogenized with the Precellys®24 system, followed by phase separation with chloroform and RNA purification using RNeasy MinElute spin columns, including on-column DNase digestion. RNA quality was assessed with the Agilent RNA 6000 Pico Kit and Agilent 2100 Bioanalyzer, with RIN values calculated to evaluate integrity.