RNA-Seq of mus musculus: granulosa cells
Construction of cDNA libraries was undertaken with a NEBNext Ultra RNA Library Prep Kit for Illumina (#E7530L, NEB, USA) according to the provided directions. The libraries were checked for size and purity with the Agilent 2100, followed by sequencing of suitable libraries on an Illumina platform and the generation of 150-bp paired-end reads.