GSM9686106: iRhot1/2-KO delivery_1; Mus musculus; RNA-Seq
Total RNA from left ventricular tissue was isolated using the NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany). The RNA sequencing library was generated from 20 ng of total RNA using the NEBNext® Single Cell / Low Input Library Prep Kit (New England BioLabs) according to the manufacturer's instructions.