RNA-seq of Paeonia rockii petal sample S3-2
Total RNA was extracted using Trizol reagent kit (Invitrogen, Carlsbad, CA, USA) according to the manufacturers protocol. RNA quality was assessed on an Agilent 2100 Bioanalyzer and RNase-free agarose gel electrophoresis. Eukaryotic mRNA was enriched by Oligo(dT) beads, fragmented, and reverse transcribed into cDNA with random primers. Second-strand cDNA was synthesized, purified, end-repaired, poly(A) added, and ligated to Illumina sequencing adapters. The ligation products were size-selected, PCR amplified, and sequenced using Illumina HiSeq 4004 by Gene Denovo Biotechnology.