RNA-Seq of Huh-7.5: Control, week 1
QuantSeq 3' mRNA-Seq FWD (Lexogen) libraries prepared from Huh7.5 cells; single-end 75 bp on Illumina NextSeq 550; libraries quantified by Qubit dsDNA HS and quality-checked on Agilent Tapestation 4200 (HS RNA and D1000 kits); pooled at 20 nM, diluted to 4 nM; loaded at 1.45 pM with 1% PhiX control.