GSM9825649: LS8817, Doxorubicin Biorep B, mH2A1, techrep 1; Homo sapiens; OTHER
LS8817 cells were briefly centrifuged followed by resuspension with ConA beads and proceeding with the rest of the CUT&RUN protocol by Epicypher (Version 2.2). CUT&RUN in approximately 100,000 cells was performed on macroH2A1 (Cat #ab37264, lot #1046297-1, Abcam), 1:100 following an optimized protocol by Epicypher (Version 2.2). NEBNext Ultra II DNA Library Prep Kit for Illumina was used for fragmentation, end repair and dA-tailing, adapter ligation and PCR amplification according to the manufacturer's instructions. Ampure XP beads were used for post-PCR cleanup. Samples were eluted in IDTE buffer.