GSM9789757: retinal organoid; No_drug + adrenaline; well A8; Homo sapiens; RNA-Seq
Total RNA isolated with the NAxtra kit; mRNA enriched with oligo-dT beads, fragmented, reverse-transcribed. 3'-barcoded bulk RNA-seq (Drop-seq-style DGE): read 1 = 12 bp per-well cell barcode (bases 1-12) + 8 bp UMI (bases 13-20); read 2 = cDNA. Libraries adaptor-ligated, PCR-amplified, pooled per organoid. NOTE: manuscript states DNBSEQ but the delivered runs are Element AVITI.