RNA-Seq of mouse neurons with h72QmP/m7Q GT in HTT gene Exp. 204
RNA was isolated using TRIzol reagent according to the manufactures instruction (Life Technologies). RNA was quantified with Nanodrop and then the integrity was evaluated. Potential contaminating DNA was removed by DNA-free kit (Ambion). Bulk RNA sequencing was performed on mWT, hHD and hHDmP cell lines (3 biological replicates per cell line) at the end of neuronal differentiation (DIV28). Stranded polyA libraries were sequenced on the Illumina NovaSeq platform (Eurofins Genomics), with an average depth of 40 million 150 bp paired-end reads per sample.