GSM8464125: P09_C7D1; Homo sapiens; Bisulfite-Seq
CD34+ cells from patients undergoing treatment with azacitidine were collected at two or four timepoints (pre-/post-treatment), and DNA and RNA extracted using the Qiagen AllPrep kit. RRBS libraries were constructed from 50ng genomic DNA using an Ovation RRBS Methyl-Seq system (Tecan). Bisulphite conversion was performed using the Epitect Fast DNA Bisulfite kit (Qiagen) and libraries amplified using the Ovation RRBS Methyl-Seq system (Tecan).