XYAP780539 (CRX1925228; CRA029192)
Nucleic acid was extracted from 300 uL of plasma using the MasterPure DNA and RNA Extraction Kit following the manufacturer's operational manual. The libraries were constructed through RNA transcription for cDNA, DNA fragmentation, end repair, adapter ligation, and PCR amplification. Then, eight uniquely barcoded libraries were pooled for hybridization and captured using specific biotinylated probes for 31 hours after library generation using the MetaCAP Pathogen Capture Metagenomic Assay Kit, following the manufacturer's instructions.