sequences of fungi
This study explores the microbial communities in two Coffea arabica L. varieties (Bourbon and PT) with different disease resistance. 16S rRNA (V4 region) and ITS1 sequencing were used to analyze bacterial and fungal communities. Samples were taken from five ecological niches: fruit, leaves, roots, rhizosphere soil, and bulk soil. DNA was extracted and PCR-amplified using region-specific primers, followed by sequencing on the NovaSeq6000 platform.