GSM9025384: Distant lymph node, patient 5; Homo sapiens; OTHER
Human fresh tissue samples were frozen and stored in OCT at −80 °C until use. Sections of 10 μm thickness were obtained using a cryostat and processed according to the manufacturer's manual (10x Genomics Visium HD Fresh Frozen Tissue Preparation Handbook, CG000763, revision D). Gene expression libraries were prepared according to the manufacturer's manual (Visium HD Spatial Gene Expression Reagent Kits User Guide, CG000685, revision B) by using human-specific probes (1000675, 10x Genomics, Pleasanton, CA, USA).