GSM9315986: Capture Hi-C IGCR1-/- rep2; Mus musculus; Hi-C
Capture Hi-C was performed using the Arima Hi-C Kit (Arima Genomics, Inc.; Cat # A510008), following the manufacturer's protocol with strict adherence to the provided instructions. To enrich the Igh locus (chr12: 113,201,001 – 116,030,000 in mm10), SureSelect Target Enrichment probes with 2× tiling density were designed and manufactured by Agilent (Agilent Technologies Inc.). Hi-C libraries were hybridized to probes as specified by the manufacturer (Agilent Technologies Inc., G9916B). Capture Hi-C libraries were prepared for sequencing using standard protcols. Libraries were sequenced using Illumina NextSeq sequencer to generate paired-end 150-bp reads.