GSM9686534: nTNKL_JP3; Homo sapiens; MeDIP-Seq
Total nucleic acids DNA were extracted from FFPE tissue sections using the RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE (Invitrogen™), according to the manufacturer's instructions. DNA concentration was measured by Qubit fluorometric quantification using the Qubit™ 1X dsDNA High Sensitivity (HS) Assay Kit (REF Q33230). DNA integrity and fragmentation were assessed using an Agilent 2100 Bioanalyzer with the High Sensitivity DNA reagents. After immunoprecipitation, captured DNA was purified using the iPure Kit v2 (Diagenode). Libraries were amplified and indexed using NEBNext® Multiplex Oligos for Illumina® and the KAPA HyperPrep Kit, followed by purification and size selection with AMPure XP beads. Sequencing was performed as 150 bp paired-end reads on an Illumina HiSeq platform.