Wild unfed (stomach)
Bacterial 16S rRNA gene amplicon libraries were prepared targeting the V3V4 hypervariable regions using primers 341F and 805R. Total DNA was extracted from the digestive tract of Meccus pallidipennis using a commercial kit following the manufacturer's instructions. PCR products were purified and quantified, followed by library preparation using the Illumina protocol for 16S metagenomic sequencing. Sequencing was performed on the Illumina MiSeq platform using paired-end.