JS-2020-7 (CRX2212867)
Mycoplasma hyopneumoniae cells were harvested by centrifugation from KM2 medium, and the DNA was extracted using an Omega Bacterial Genomic DNA Extraction Kit. The DNA was then randomly sheared to 350 bp using a Covaris instrument. Subsequently, the sequencing library was constructed through steps including end-repair, A-tailing (or 3' end A-tailing), and adapter ligation, followed by library fragment size selection.