GSM9079334: tumor sphere; Homo sapiens; RNA-Seq
Organoid slices (n = 3), organoids (n = 3), assembloids (n = 3), tumor spheres (n = 6), each pooled from the same biological replicate in one tube, and tumor-like cells (n = 1), were washed in cold PBS at 300xg, 5 min. RNA was isolated as described in a section “Real time RT-PCR analysis” and stored at -80 °C. Preparation of samples for sequencing including the isolation of the nuclei, and initial quality control were performed as a service by GENEWIZ/AZENTA (USA). Library preparation, sequencing and initial data processing were performed as a service by GENEWIZ/AZENTA (USA). The standard RNA-seq bundle with polyA selection with 2x150 bp was performed on the Illumina series. The estimated sequencing output was ~50 million paired-end reads per sample. Library preparation, sequencing and initial data processing were performed as a service by GENEWIZ/AZENTA (USA). The RNA-seq library was prepared using 10X Genomics Chromium® 3` expression, and the 10X library sequencing was performed on the Illumina series. The target number of cells per sample was 6000, and number of reads per cell 50000.