ctrl-1 (CRX1237592; CRA019936)
Primary neurons were seeded into 6-well plates and cultured for 24 h. The culture medium was then replaced with glucose-free DMEM to initiate glucose deprivation. Subsequently, the plates were incubated anaerobically for 6 hours using the AnaeroPack system to induce OGD. Afterwards cells were collected for RNA-seq.