ONT sequencing of human rob(13;14) sample
=2 ug of high molecular weight genomic DNA (more than 50% of fragments =40 kb) was sheared to ~20 kb using the Megaruptor (Diagenode B06010002), followed by DNA repair and ligation of ONT adapters using the ONT Ligation Sequencing Kit (SQK-LSK109). Short fragments were removed in a size selective bead cleanup included in the kit. The ligated libraries were quantified using the Qubit dsDNA High Sensitivity assay (Thermo Q32854) and libraries were prepared for sequencing using ONT provided buffers (EXP-AUX002).