GSM9789810: Human PBMCs CITE-seq library, 10X Batch 2, GEX; Homo sapiens; OTHER
Peripheral blood mononuclear cells (PBMCs) were separated and cryopreserved from blood samples collected at baseline and 7-days post-vaccination PBMC samples were collected from 10 patients with SAD, 10 CVID and 10 healthy controls collected before and 7 days after vaccination. All 60 samples were stained with Hash Tag Oligonucleotides (HTOs) and a panel of 130 Antibody-Derived Tags (ADTs) using TotalSeqTM antibodies (BioLegend), pooled and then split into 5 batches (12 samples each). For each batch, GEX, ADT and HTO and VDJ libraries were generateed using the 10X Genomics Chromium single-cell 5′ reagent kit v3 according to manufacturer's protocol and then sequenced on a NovaSeq X Plus (PE150).