GSM8464024: marginal zone B cell, anti-Dll4, 48h, rep4; Mus musculus; RNA-Seq
Pure cell populations were FACS-sorted into Trizol reagent supplemented with 0.5% 2-mercaptoethanol. Phenol-Chloroform RNA isolation and precipitation was performed with glycogen carrier according to manufacturer protocol. cDNA was prepared using Takara Clonetech SMART-seq HT kit and Illumina Nextera XT kits according to SMART-seq HT kit protocol.