RNA_seq_MSQ43_Rep2
Cells were grown at approximately 60% confluence, washed twice with 1x PBS, harvested with TripleExpress solution (Thermo Fisher Scientific, #12604013), counted, and transferred to 1.5 ml tubes. Total RNA was extracted using Aurum Total RNA Mini Kit (Bio-Rad, #7326820). 1-2 mln cells were lysed followed by purification on silica membrane in a spin-column format using a spin-mediated protocol, including DNA digestion for 25 minutes. Concentration was measured with a Qubit 4 spectrophotometer using Qubit RNA HS Assay kit (Thermo Fisher Scientific, #Q32852), then samples were sent for RNA sequencing to the BGI facility (Hong Kong).