GSM9241512: ESC_WT_biol_rep_1; Mus musculus; RNA-Seq
RNA was extracted using the AllPrep DNA/RNA Mini kit Library prep was done using the UCLA TCGB core facility. Briefly, poly(A) RNA were selected, fragmented and double-stranded cDNA synthesized using a mixture of random and oligo(dT) priming, followed by end repair to generate blunt ends, adaptor ligation, strand selection, and polymerase chain reaction (PCR) amplification to produce the final library