GSM9168418: CL002 T-cells, Infected Macrophages, GEX; Homo sapiens; RNA-Seq
Activated CD4+ were resuspended in 0.04% BSA in PBS Sequencing of mRNA from single T cells was performed using the 10x Genomics Chromium Next GEM Single Cell 5' V2 platform (10X Genomics, Pleasanton, CA, USA). Activated CD4+ were resuspended in 0.04% BSA in PBS and loaded onto the 10X Chromium Controller. RNA was reverse-transcribed, and cDNA was isolated and PCR-amplified according to the 10X Genomics NextGEM 5' V2 scRNAseq User Guide (Rev D). Quality control and quantification of cDNA and final libraries was performed on either the Agilent fragment analyzer or Agilent 2100 Bioanalyzer (Agilent technologies, Santa Clara, CA, USA). V(D)J amplification and library preparation, and gene expression library construction were performed using 10X Genomics kits according to the User Guide.