WGS of NM04 bacterium
Single colonies grown on M9 + 1g/L nicotine agar plates were inoculated into 3 mL of LB and incubated overnight before pelleting and washing with 1 mL of DPBS and then resuspending in 500 L of Zymo DNA/RNA Shield. DNA was extracted using the Zymo Quick-DNA Miniprep Plus kit. Sequencing library preparation and genome sequencing were performed by Plasmidsaurus using Oxford Nanopore Technology (ONT). Libraries were constructed with v14 library prep chemistry, and sequencing was performed on R10.4.1 flow cells without primers.