GSM8515102: CTRL_THP1_RNA-Seq_Rep2; Homo sapiens; RNA-Seq
1 million cells from each clone were used for total RNA extraction using RNeasy mini kit (Qiagen 74106) following the manufacturer recommended protocol. RNA concentration was determined by using Agilent 2200 TapeStation and a high sensitivity RNA Screentape (Agilent 5067-5579). Samples with RIN greater than 8 were used to construct RNA-Seq libraries. RNA libraries were prepared using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB E7530L) using the manufacturer recomended protocol. Concentrations and sizes of libraries were determined using Agilent 2200 TapeStation and KAPA library quantification kit (Roche KK4824). RNA libraries were prepared using  NEBNext Ultra RNA Library Prep Kit for Illumina (NEB E7530L) using the manufacturer recomended protoco. Concentration and the size of the libraries were detremined using Agilent 2200 Tapestation and KAPA library quantification kit (Roche KK4824). Libraries from all samples were pooled in equimolar concentrations and denatured for paired-end sequencing. Pooled libraries were quantified using KAPA library quantification kit (Roche KK4824).