GSM9742238: C. elegans_BW25113_4; Caenorhabditis elegans; RNA-Seq
RNA was extracted from C. elegans samples using the TransZol UP Plus Kit. After passing the quality control via electrophoresis, the extracted C. elegans RNA underwent purification, rRNA removal, fragmentation, cDNA synthesis, and end repair processes using the RNA Clean XP Kit and RNase-Free DNase Set. The concentration of the constructed libraries was measured using a Qubit2.0 Fluorometer, and their size was detected with an Agilent2000. Finally, the libraries were sequenced on an Illumina NovaSeq6000 sequencer in PE150 mode.