Effect of aptamer treatment on AML cells
Effect of aptamer treatment on AML cells
Cells were collected by centrifugation post experimental time points and stored and shipped at -80 C  to the sequencing facility. Cells were grown in HPLM media supplemented with 10% fetal bovine serum with antibiotic-antimycotic solution. Aptamers were stored in 10 mM HEPES buffer (pH 7.2) containing 100 mM NaCl and 12 mM MgCl2 and cells were incubated in the same buffer during the treatment period of 6 h. RNA was extracted using Trizol following manufacturers protocol. Sequencing library was constructing using polyA enrichment PCR.