GSM9263537: D3_9AM_erbb3_FF0701; Mus musculus; RNA-Seq
Sample preparation was performed using the GEM-X Flex Sample Preparation v2 Kit (1000781, 10x Genomics) according to the manufacturer's protocol. Endometrial tissues (~25 mg) from Erbb3f/f and Erbb3d/d females were dissected on ice, finely minced, and fixed in Fixation Buffer B for 16 h at 4 °C. Samples were subsequently washed with PBS, quenched in Quenching Buffer B, and enzymatically dissociated in Dissociation Solution containing collagenase I, collagenase IV, and P&S at 37 °C for 40 min with intermittent pipetting. The resulting cell suspensions were passed through a 40 μm strainer, washed, and either processed immediately for library construction or stabilized with Enhancer and 50% glycerol for storage at −80 °C. Single-cell libraries were generated using the Chromium Next GEM Single Cell 3' Gene Expression Flex v2 kit (10x Genomics) and sequenced on an Illumina platform. Single-cell suspensions were processed using the 10x Genomics Chromium Flex Gene Expression workflow, following the manufacturer's instructions. Libraries were constructed with the Chromium Next GEM Single Cell 3' Kit v3.1 (10x Genomics).