DAP-seq of Morella Rubra at T01
DAP-seq was performed. The MrMYB6 CDS was cloned into the pFN19K vector. MrMYB6 was expressed with a TnT Coupled Wheat GermExtract System (Promega). gDNA was extracted from leaves harvested from 3-month-old Myricarubra with a DNeasy Plant Mini Kit (Qiagen, Germany). gDNA was sonicated to a fragment size of 200 to 800 bp. Halo-MrMYB6 protein was bound to anti-Halo monoclonal antibody agarose beads (Promega) and incubated with 200 ng fragmented gDNA for 1 h at room temperature. The beads werewashed, and DNA was recovered. Samples were pooled and sequenced on an Illumina Novaseq 6000 platform; 150 bp paired-end reads were generated from each library. A total of 1030 millionreads were obtained for each sample.