GSM9022798: ICAM1 KO-1; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol reagent following the manufacturer's protocol. For CUT&Tag, nuclei were isolated and tagmented according to the CUT&Tag protocol. RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit. CUT&Tag libraries were prepared using the Illumina