GSM9315808: Early-passage HUVECs, Rep F; Homo sapiens; miRNA-Seq
Total RNA was extracted from the cell pellets using the MagMAX™ mirVana™ Total RNA Isolation Kit (Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer's instructions for ECs. Libraries of small RNA were constructed using NEXTFlex Small RNA-Seq Kit v4 (Illumina Compatible) (PerkinElmer, Waltham, MA, USA), according to the manufacturer's guidelines. Size selection was performed targeting fragments that were 150–180 bp in size using BluePippin (Sage Science, Beverly, Massachusetts, USA). Size‐selected libraries were then PCR amplified with indexed sequencing primers, and finally sequenced on a NovaSeq X Plus System (Illumina, San Diego, California, USA) for 150x2 cycles in high‐output mode with target coverage of 20 M reads for each library.