GSM8795893: sample1 RNF43 scRNAseq; Homo sapiens; RNA-Seq
Samples were processed according to an in-house developed protocol and the 10x Genomics Demonstrated Protocol Cell Preparation Guide (Document CG00053). Briefly, after removal of the supernatant, T-cells were gently washed twice in 1.5–3 ml of 0.04% BSA in PBS. Additionally, cells were passed through a 35μm strainer (BD Falcon® 5 ml Round-Bottom Tubes with Cell Strainer Cap, Corning, NY, USA) to eliminate cell clumps. Next, cells were stained with 0.4% Trypan blue and quantified and assessed for viability using the automated cell counting machine Countess 3 (Invitrogen, FL, US). Single-cell RNA-seq (scRNA)) analyses were performed using the 10x Genomics Single Cell Immune Profiling Solution V1.0 according to the manufacturer's protocols (10x Genomics V(D)J + 5′ Gene Expression).