GSM8635227: IAA-1-3; Homo sapiens; Hi-C
Briefly, five million CTCF-AID knock-in HEL cells without or with IAA treatment for 24 hours were crosslinked with 1% formaldehyde for 10 min at room temperature, digested with 125 units of MboI, labeled with biotinylated nucleotides and ligated for proximity ligation. After de-crosslinking, the ligated DNA was purified and sheared to 300–500 bps. Ligation junctions were pulled down with streptavidin beads and prepared as a standard Illumina library. Each library underwent 75 cycles of paired-end sequencing on the Illumina HiSeq 4000 system.Each sample had two replicates, and we merged the replicates after confirming reproducibility. Each sample contained > 250 million paired-end fragments, > 60% ligation motif present, and > 200 million contacts.