Direct targeting of SLC7A11 by miR-143-3p promotes ferroptosis and suppresses malignancy in cervical squamous cell carcinoma
Direct targeting of SLC7A11 by miR-143-3p promotes ferroptosis and suppresses malignancy in cervical squamous cell carcinoma
DNA was extracted from tissues and cells using the EasyPure® Genomic DNA Kit (without RNase A), following the manufacturer's instructions. DNA was extracted from tissues and cells using the EasyPure® Genomic DNA Kit (without RNase A), following the manufacturer's instructions. Amplification was performed using the 2×Easy Taq PCR Super Mix Kit in a 25 μL reaction mixture comprising 2 μL DNA template, 0.5 μL each of forward and reverse primers (50 ng/μL), 12.5 μL of 2× PCR Mix, and 9.5 μL of sterile double-distilled water.