GSM9436215: microdisected facial tissue, E11.5, control; Mus musculus; RNA-Seq
Following microdissection, lambdoidal junction tissues from 2 embryos were pooled together and treated with 750µl of 0.25% trypsin-EDTA at 37°C for 10 mins with gentle agitation. The reaction was stopped by adding 750µl of DMEM with 10% FBS. The mixture was then pipetted up and down 20 times with wide orifice tips to assist in cell dispersion, and the cells were collected by centrifugation at 300 g for 3 mins. Cells were washed in PBS with 0.4% BSA twice, and then filtered through Flowmi 40µM cell strainer before proceeded for single cell RNA-seq. Libraries were prepared uing the 10xGenomics Chromium Next GEM 3′ v3.1 kit following the manufacturer's protocol.