GSM9105365: Eko male mice, 1707; Mus musculus; RNA-Seq
Total RNA was extracted using the RNeasy Fibrous Tissue Mini Kit following the manufacturer's protocol. The quality of RNA was assessed by 2100 bioanalyzer (Agilent), and RNA with RIN >7.5 was utilized for RNA sequencing using polyA selected RNAs. The cDNA libraries were created following the Illumina TruSeq Stranded mRNA library preparation protocol using 250 ng of total RNA for each sample. The generated libraries were quantified with a NanoDrop spectrophotometer, and their fragment sizes were evaluated using an Agilent Bioanalyzer. The libraries were then pooled and loaded onto a NovaSeq 6000 S4 flow cell, where they were sequenced to a depth of approximately 100 million read pairs per sample. A 150-cycle paired-end run was employed for sequencing on the NovaSeq 6000 system.