GSM9686976: Lac, 3 weeks, bio rep 1 and 2; Rattus norvegicus; RNA-Seq
Pubocaudalis muscle was extracted and stored in RNAlater at -80C until RNA isolation. 30 mg of tissue was excised and underwent RNA isolation using Rneasy fibrous tissue mini kit (Qiagen). mRNA was checked for degradation using the TapeStation 4200. RNA was then pooled with two biological replicates per RNA pool. Pools were quantified using a Qubit 3.0 with a Qubit™ RNA High Sensitivity kit. Samples were prepared using the Illumina Stranded mRNA kit according to manufacturer protocols. Final libraries were sizes using a D1000 ScreenTape on a TapeStation 4200 and quantified using a Qubit 3.0 with a Qubit™ 1x ds DNA High Sensitivity kit. Libraries were pooled and sequenced on one lane of the 10B PE100 flow cell using the Illumina NovaSeq X Plus.