3' single cell sequencing of CD45+ lymphocytes sorted from mesenteric white adipose tissue in wildtype C57BL/6 and WIPf1-/- mice
3' single cell sequencing of CD45+ lymphocytes sorted from mesenteric white adipose tissue in wildtype C57BL/6 and WIPf1-/- mice
Mesenteric white adipose tissue from wildtype C57BL/6 and Wipf1-/- mice was harvested through vivosection, minced and digested. Tissue-resident lymphocytes were enriched with Percoll gradient centrifugation. CD45 staining and fluorescence associated cell sorting were performed to achieve a pure lymphocyte population.Single cell suspension was made with 20.000 of these cells on the 10x genomics platform and following the Chromium Next GEM Single Cell 3' Reagent Kit User Guide Revision E. Nucleic acid extraction was performed following the Chromium Next GEM Single Cell 3' Reagent Kit User Guide Revision E.  Library preparation was performed following the Chromium Next GEM Single Cell 3' Reagent Kit User Guide Revision E. 