GSM9469752: C. trachomatis, EB, BPDL 24hpi, rep1; Chlamydia trachomatis; RNA-Seq
Total RNA was extracted from infected HEp-2 cells using TRIzol™ (Invitrogen) followed by DNase I digestion (Invitrogen) and purification according to the manufacturer's instructions. RNA quality was verified with a Fragment Analyzer (Agilent) and quantity determined using the Qubit™ RNA Broad-Range Assay Kit (Thermo Fisher). RNA was stored at –80°C until library preparation. rRNA was removed using the Ribo-Zero rRNA Removal Kit (Illumina). cDNA libraries were prepared using the Illumina TruSeq Stranded mRNA Library Prep Kit with poly-A negative selection. Libraries were sequenced on an Illumina NovaSeq 6000 platform in paired-end mode.