GSM9896272: BG5 PDGC, 90k seeding, R1, 55-TF OE screen; Homo sapiens; RNA-Seq
PDGCs were isolated with FACS, fixed with ScaleBio Sample Fixation Kit  (PN2020001) and stored at -80C until later processing. RNA libraries were prepared with the Single Cell RNA Sequencing Kit v1.1 (PN950884) + Extended Throughput kit v1.1 (PN945058), with custom primers spiked-in to improve capture of barcode mRNA during reverse transcription. Barcode fragments in final RNA library were PCR amplified and spiked in at 1:10 ratio with the RNA library to improve barcode detection.