RNA-seq of P. aeruginosa in response to Pyocin L1
500 L samples were taken at 15 min and 120 min timepoints and added to RNA Protect solution (Qiagen). The samples were left for 5 min at room temperature, spun down, supernatant removed, and then frozen at 80C until the RNA was extracted. The untreated control was only sampled at the 15-minute timepoint. The Qiagen RNAprotect Bacteria Reagent Handbook protocol 4, protocol 7 and on-column DNase digestion using the Qiagen RNase-free DNase set were followed. Sequencing libraries were prepared using an Illumina TruSeq RNA Prep kit according to the manufacturers specifications.