GSM8689849: K562, WT, E50_dCas9-KRAB, sgRNA_Control; Homo sapiens; RNA-Seq
RNA was isolated using conventional TRIzol extraction and isopropanol precipitation. 1ug purified RNA per sample was used as input for NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, cat. no E7490) to enrich for polyA mRNA. Sequencing libraries were then produced using NEBNext Ultra II Directional RNA Library Prep Kit (NEB, cat. no. E7645). Sequencing libraries were pooled and paired-end sequenced (2 × 100 bp) on an Illumina Nextseq 2000 platform.