GSM9744030: LPS 2; Mus musculus; RNA-Seq
Total RNA was extracted from rat supraspinatus muscle tissues using TRIzol reagent according to the manufacturer's instructions. RNA quality and quantity were assessed before library construction. mRNA was enriched using oligo(dT)-conjugated magnetic beads for downstream transcriptome sequencing. Sequencing libraries were constructed from enriched mRNA. Briefly, mRNA was fragmented and reverse-transcribed into cDNA. RNA-seq libraries were generated by adaptor ligation and PCR amplification, followed by paired-end sequencing on an Illumina NovaSeq 6000 platform.