Amplicon sequencing bulk soil grassland
Soil DNA was extracted with the DNeasy PowerSoil Pro Kit (Germantown, PA) according to the manufacturer's guidelines. The extracted DNA samples were assessed using a Nanodrop ND-1000 (Thermo Scientific: Ramsey, MN). The average ratios of 260/280 and 260/230 for the DNA samples were 1.920 and 1.985, respectively. Microbial communities were assessed by sequencing 16S rRNA, ITS, and protist 18S rRNA using the primer sets 515F (Parada et al., 2016) and 806R (Apprill et al., 2015), ITS1F and ITS2 (White et al., 1990), and 1391F (77) and EukBr (Amaral-Zettler et al., 2009), respectively. Sequencing occurred at the University of Minnesota Genomics Center in Minneapolis, MN, employing the Illumina MiSeq V3 300 bp paired-end technology. Libraries were prepared following a modified protocol outlined by Gohl et al. (2016).