16S rRNA sequencing of fermentation corn stalk microbiome by 3 day
Microbial DNA was extracted from corn stover fermentation samples. The V3V4 hypervariable regions of the 16S rRNA gene were amplified using primers 341F and 806R. PCR products were purified, quantified, and pooled in equimolar concentrations. Libraries were prepared according to the Illumina 16S Metagenomic Sequencing Library Preparation protocol and sequenced on an Illumina platform using paired-end 2248 bp reads.