RNA-seq of mus musculus:2 months male tails
Total RNA was extracted from mouse tail tissues using TRIzol reagent. RNA quality was assessed (RIN  7). For each sample, 1 g of total RNA was used for library preparation with the VAHTS Universal V8 RNA-seq Library Prep Kit. Ribosomal RNA was depleted, the remaining RNA was fragmented to ~350 bp, and cDNA was synthesized. Adapters with sample-specific i7 indices were ligated, and libraries were amplified, purified, and validated on an Agilent 2100 Bioanalyzer.