GSM9320588: AD205-60A; Synechococcus elongatus PCC 7942 = FACHB-805; RNA-Seq
Total RNA was extracted using RNeasy kit with QiaShredder columns (QIAGEN). Cells were lysed in RLT buffer containing 1% β-mercaptoethanol, homogenized through QiaShredder, then processed following manufacturer's protocol with 70% ethanol addition. Library construction and sequencing was done by Azenta Life Sciences. Two technical replicates were processed per condition. Samples underwent DNase treatment and rRNA depletion before paired-end sequencing (2x150bp, ~350M reads, ~105GB) with single indexing on Illumina platform.