GSM9024929: 5-C01D02; Homo sapiens; RNA-Seq
10mL of whole peripheral blood (collected in PAXgene tubes) were collected for each sample and cryopreserved at -80C. The PreAnalytix PAXgene Blood RNA  kit was used to extract RNA and an RNA-integrity number of 8 or above (measured using a bioanalyzer) was ensured prior to library preparation. The construction of mRNA libraries was performed according to the Illumina TruSeq V2 library preparation kit. The validation of these libraries occurred through microelectrophoresis on a 2100 Bioanalyzer system (Agilent), quantification using Kapa Library Quantification Kits (Roche), and eventual pooling and clustering on Illumina TruSeq V2 flow cells.