T4 sample for RNA-Seq of swabs from surface of Sargassum sp.
The V3-V4 hypervariable region of the 16S rRNA gene was amplified using the 341F (CCTACGGGNGGCWGCAG) and 805R (GACTACHVGGGTATCTAATCC) primers. PCR was performed in 50 L reaction mixtures containing 5 L of DNA template, 25 L of Perfecta SYBr Master Mix and 5 L of VeritiPro Thermal Cycler with an initial denaturation at 95C for 3 minutes, followed by 30 cycles of 95C for 30 seconds, 55C for 30 seconds, and 72C for 5 minutes.