GSM8516018: Pre-GMP-3_edit_1; Homo sapiens; OTHER
CD34+ HSPCs edited with Cas9-3xNLS targeting HBG promoter, cultured in X-VIVO media with cytokines, and harvested cells at day 5 after electroporation. Genomic DNA extracted from control and edited cells using Agencourt DNAdvance according to manufacturer's protocol. Genomic DNA from edited CD34+ normal cells or unedited control cells (Pre-GMP engineering runs, n = 3) used to perform CHANGE-seq. Pooled libraries were sequenced with 151 bp paired end reads on Illumina Novaseq 2000.