GSM9026135: JWJ-01-306, 5 days, Rep3; Homo sapiens; RNA-Seq
Total RNA was extracted from cells using the Direct-zol RNA Miniprep Plus kit (Zymo Research #2072) PolyA RNA isolation and library preparation was performed with the Watchmaker mRNA Library Prep Kit (Watchmaker Genomics: 7BK0001 ) with xGEN Stubby adaptors (IDT, Cat: 336338420) and xGEN 10nt UDIs (IDT, Cat: 336338436). Libraries were sequenced with the Element Biosciences AVITI Sequencing platform using the AVITI 2x75 High Output Cloudbreak Freestyle Kit (Element Biosciences, Cat: 860-00015).