GSM8691397: adult zebrafish retinal ganglion cells, day 7 uninjured, fish set #2; Danio rerio; RNA-Seq
1 and 7 days post injury (dpi), the retina were harvested, cells dissociated using papain digestion, and RGCs were FACS isolated into BSA/PBS for downstream processing using the 3' v2 kit (10X genomics) Single cell libraries were prepared using the single-cell gene expression 3' v2 kit on the Chromium platform (10X Genomics, Pleasanton, CA) following the manufacturer's protocol. Briefly, single cells were partitioned into Gel beads in EMulsion (GEMs) in the Chromium instrument followed by cell lysis and barcoded reverse transcription of RNA, amplification, enzymatic fragmentation, 50 adaptor attachment and sample indexing. On average, approximately 7,000 single cells were loaded on each channel and approximately 2,000- 4,000 cells were recovered. Libraries were sequenced on NovaSeq S2-100 platform (Paired end reads: Read 1: 26 bases, Read 2: 98 bases).