GSM9760169: LLC wild-type cells, biol. rep. 3; Mus musculus; RNA-Seq
LLC WT and LLC Ago2 KO cell lines were maintained in culture as previously mentioned. RNA was purified using NucleoSpin® RNA kit (MACHEREY-NAGEL) following the manufacturer's protocol. Total RNA quality was analysed using the Agilent 2100 Bioanalyzer G2939A. RNA libraries were prepared from total RNA using the Stranded mRNA Prep Ligation kit (Illumina) following the manufacturer's instructions. Libraries were sequenced on an Illumina NovaSeq 6000 (S1 flow cell) in paired-end 100 bp mode, targeting 50 million reads per sample.