RNA-seq of K562 cells: Difluoromethylornithine (DFO)
K562 cells were treated as indicated for 72 hours, pelleted, and stored at -80C until use. Plasmidsaurus performed RNA extraction from cells stored in DNA/RNA Shield (Zymo), after which libraries for differential gene expression were prepared using BRB-seq, which employs barcoded poly-T primers to capture polyadenylated transcripts, which are reverse transcribed. The resulting double-stranded cDNA was tagmented and adapters were added during PCR amplification.