GSM8723660: Mouse, skin, PD4, scATAC, rep1; Mus musculus; ATAC-seq
The entire back skin was harvested from sacrificed mice, and the epidermis was separated from the dermis by incubation with 1 mg/mL Dispase II (Roche) at 37°C for 30 minutes to 2 hours. The dermis was subsequently digested with 0.125 mg/mL Liberase TL (Roche) at 37°C for 30 minutes, while the epidermis was digested with 0.25% trypsin (Gibco) at 37°C for 15 minutes. Dissociated cells were filtered sequentially through 100-μm and 40-μm strainers, followed by a 1:1 matching of epidermal and dermal cells. ScATAC-seq data were generated using 10x Chromium Single Cell ATAC Reagents Kits (v1 and v2), while scRNA-seq data were generated using the 10x Chromium Single Cell 3' Kit (v3.1), following the manufacturer's instructions. Libraries were sequenced on a Novaseq system (Illumina), and two separate datasets, including replicates, were generated for each time point.