GSM9897630: BHK-21 37C before cold rep3; Mesocricetus auratus; RNA-Seq
Cell pellets were lysed and homogenized using QIAshredder columns (Qiagen), and RNA was extracted using the RNeasy Mini Kit (Qiagen) with on-column DNase digestion, according to the manufacturer's instructions. Libraries were prepared using the NEBNext Ultra II kit. In brief, 1 ug of RNA per sample underwent Poly(A) selection before fragmentation and random priming followed by first- and second-strand complementary DNA synthesis. Following cDNA synthesis, NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs, NEB E6441A) were ligated before PCR enrichment. All libraries were pooled to a final concentration of 2 nM and sequenced on a NovaSeq 6000.