GSM8691467: CD4,KO,infected, oligo; Mus musculus; RNA-Seq
Single-cell suspensions were prepared from harvested lung and spleen tissues. Samples were processed using mechanical dissociation and enzymatic digestion to ensure viable single-cell isolation. Dead cells and debris were removed using density gradient centrifugation. Single-cell RNA-seq libraries were constructed using the BGI C4 single-cell sequencing platform. The libraries were generated following the C4 protocol, which uses droplet-based single-cell capture, UMI barcoding, and cDNA synthesis. Libraries were then sequenced on the BGI DNBSEQ-G400 instrument.