GSM9540953: CSC-A /Timepoint1/Replicate2/Media=B4U Run 2 (B4 with urea); Curtobacterium flaccumfaciens; Microbacterium foliorum; Bacillus toyonensis; Rhodococcus qingshengii; RNA-Seq
RNA extraction was performed using Zymo's Quick-DNA/RNA Miniprep Kit (D7003) and sequenced by Azenta (South Plainfield, NJ) using Illumina technology. Bioconductor's DESeq2 package (3.9)26 was used to analyze gene expression for each member of the CSC-A community under the different conditions tested (in the presence and absence of C. flaccumfaciens and R. qingshengii or in different media types). NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina