GSM8464870: control group rep3; Mus musculus; RNA-Seq
The total RNA of the sample was isolated and purified by TRIzol (Invitrogen, 114 CA, USA). The mRNA captured by oligo(dT) magnetic beads was fragmented at high temperature and reverse transcribed into cDNA. Next, the second strand of cDNA was synthesized by RNase H digestion, and the ends of the double-stranded DNA were aligned to form flat ends. Then, an A base was added at each end of the double-stranded DNA so that it could be ligated to the junction with a T base at the end of the double-stranded DNA. Finally, magnetic beads were used for the selection of fragment sizes and purification so that fragments could be formed into 300 bp±50 bp fragments.