Whole genome sequencing of single neuron MDA amplified DNAfrom homo sapien Brodmann area 6
Single neuronal nuclei were isolated using FANS with staining of NeuN antibody from frozen brain (Brodmann area 6). The nuclie were lysed and the genome amplifeid using multiple displacement amplification (MDA). Libraries were prepared using Illumina TruSeq DNA PCR-Free preparation kits following the manufacturer protocol, sequencing was performed to a target depth of 45X on an Illumina NovaSeq 6000.