RNA-seq of TIB190 cell line treated with kifunensine
Gene expression was profiled using 3 Tag-Seq (UC Davis DNA Technologies Core). Total RNA was purified and polyadenylated transcripts were selectively captured using oligo-dT priming. Unique molecular identifiers (UMIs) were incorporated during reverse transcription to enable PCR duplicate removal. Strand-specific cDNA libraries were generated, and sequencing was performed as single-end ~80 bp reads, targeting 3 ends of transcripts for accurate gene-level quantification.