CTRL
We extracted total RNA from flash frozen copepods using a combination of TRIzol reagent (Invitrogen; catalog no. 15596026) and the Qiagen RNAeasy Plus kit (Qiagen, catalog no. 74134). Briefly, copepods were homogenized using a TissueRuptor II (Qiagen, catalog no. 9002755) before we followed steps 17 of the TRIzol protocol, followed by steps 411 of RNAeasy Plus kit protocol. Total RNA extracted from a total of 15 ((4 timepoints + 1 control) x 3) samples were sent to Novogene Corporation Inc. at Sacramento, California, where RNA quality control was confirmed using a 2100 Agilent Bioanalyzer on a Eukaryote Total RNA Nano chip and nondirectional libraries were produced using polyA tail selection. The resulting 15 libraries were sequenced on NovaSeq X Plus, with 150bp pairedend reads.