GSM8464549: LuCaP96CR_Hi-T_scRNA; Homo sapiens; RNA-Seq
Tissue fragments were incubated in 3 ml Accumax (Innovative Cell Technologies, AM105) for 10-20 min at room temperature on a rocking shaker. Red blood cells were lysed with ACK Lysing Buffer (Thermo Fisher Scientific, A1049201) on ice for 1 min, followed by quenching with PBS and then filtered again with a 40-μm cell strainer. Cell suspensions were washed three times and resuspended with PBS plus 0.04% BSA. The final cell viability of suspensions was determined by Countess Automated Cell Counter (Thermo Fisher Scientific). CDNA