W7_cKO3 (CRX1826070; CRA027416)
The library preparation was conducted following the manufacturer's instructions (10x Genomics). Initially, total cells were resuspended in the master mix and co-loaded with partitioning oil and gel beads. Subsequently, the poly-A RNA extracted from the cell lysate was reverse-transcribed into cDNA, incorporating an Illumina R1 primer sequence, a unique molecular identifier (UMI), and the 10x Barcode. The barcoded cDNA pool was then purified, PCR-amplified, and appropriately-sized fragments were selected using SPRIselect reagent for subsequent library construction.