GSM9316560: hybrid_embryo_female_litter#9_individual#E1; Mus musculus domesticus x Mus spretus; RNA-Seq
Total RNA was extracted from hypothalamus tissue using the AllPrep RNA/DNA Mini Kit (Qiagen), and was stored at -80°C until sequencing. Illumina library preparation was done at the sequencing facility (Novogene, Sacramento, CA) using the NEB Next Ultra RNA library prep kit for Illumina.  Individual libraries were sequenced on the Illumina HiSeq 4000 platform, producing >40 million, 150bp paired-end reads per sample.