Cn5
Four fluorescently labeled dNTPs, DNA polymerase, and adapter primers are added to the flow cell for sequencing to carry out amplification. When the complementary strand is extended in each sequencing cluster, each addition of a fluorescently labeled dNTP releases the corresponding fluorescence. The sequencer captures the fluorescent signals and converts the optical signals into sequencing peaks through computer software, thereby obtaining the sequence information of the fragment to be tested.