GSM9500909: worms-L4440-ERCC; Caenorhabditis elegans; RNA-Seq
Total RNA from cells or C. elegans strains was extracted by TRIzol Reagent or TRIzol LS Reagent according to the manufacturer's procedures. The concentration of isolated RNAs was verified by Nanodrop, and RNA quality was checked with gel electrophoresis. For poly(A)-selected RNA-seq, external RNA Control Consortium (ERCC) spike-in RNA controls (Invitrogen, 4456740) were diluted and added according to the manufacturer's instructions. Specifically, 1 μL of 1:100 diluted ERCC spike-in RNA mix was added to 5 μg total RNAs prior to enrichment using oligo(dT) magnetic beads and library preparation.