GSM9828164: WT_D4_MCnT_H3K27ac_rep1; Mus musculus; OTHER
Nuclei were prepared from 500,000 cells (H3K4me1) or 1,000,000 cells (H3K27ac) for each transposition reaction. A transposome was assembled with methylated adaptors for transposition reaction. Tagmented purified DNA was either bisulfite converted (Zymo EZ DNA Methylation-Gold Kit) for H3K4me1, or submitted to Enzyme-Methyl kit (NEB) for H3K27ac. Converted DNA was amplified using KAPA HiFi HotStart Uracil+ ReadyMix.