Whole transcriptome sequencing of patient-derived preclinical models: Sample 772611-094-R-ORIGINATOR-RNA
"RNASeq libraries were prepared using 20-100 ng of total RNA using Illumina Truseq RNA Exome kit. cDNA synthesis from fragmented RNA was followed by dA tailing, ligation with indexed adaptors and subsequent PCR amplification to enrich adapter-attached fragments. Amplified libraries were hybridized to capture probes targeting the exome. Streptavidin-coated magnetic beads were then used to capture the probe-bound libraries, followed by amplification to generate the final libraries. The final libraries were quantitated and sequenced. The whole library preparation steps were automated using liquid handlers."