GSM9708881: B16.F10 melanoma cells, Simvastatin 3.5 uM, 24h_replicate1; Mus musculus; RNA-Seq
B16.F10 cells were seeded as 1x10^6 cells/flask and treated with 3.5 µM SIM for 24h at 37°C. Then, the RNA isolation was performed using peqGOLD Total RNA isolation kit (PeqLab, Erlangen, DE), including the DNase digestion treatment of the lysates (peqGOLD DNase I Digest Kit, PeqLab, Erlangen, DE), to avoid DNA contamination, according to the manufacturer's instructions [160]. The RNA was eluted with 50 μl of RNase-free water. To generate strand-specific cDNA libraries, TruSeq Stranded Total RNA (Illumina, Inc.) was used followed by quality control procedure (Qubit 2.0 Fluorometer, Thermo Scientific, USA; Agilent 2100 bioanalyzer, Argilent Technologies, USA).