GSM9862542: Library 6 CITE-seq CD8+ T cells, Gene Expression; Mus musculus; RNA-Seq
Single-cell suspensions were prepared from tumors and lymphoid tissues by mechanical and enzymatic dissociation. CD8+ T cell populations were enriched and stained with fluorophore-conjugated antibodies and TotalSeq™ oligonucleotide-conjugated antibodies. Cell populations were sorted by flow cytometry based on tdTomato, LAG3, CD44, and additional surface markers. Viable sorted cells were immediately processed using the 10x Genomics Chromium platform. Single-cell libraries were generated using the 10x Genomics Chromium Single Cell 5' Library & Gel Bead Kit according to the manufacturer's protocol. Three libraries were generated from each sample: (1) Gene Expression (GEX), (2) Feature Barcode libraries for CITE-seq (TotalSeq antibodies), and (3) V(D)J TCR libraries. Libraries were sequenced on an Illumina NovaSeq 6000 platform.