HiFi Analysis for Anisodus tanguticus: Old and Young Plants
After the detection, the DNA was purified using AMPure PB beads (Pacbio 100-265-900), and the final high-quality gDNA was used for subsequent library construction. PacBio SMRTbell (Single-molecule real-time) library was constructed using SMRTbell Express Template Prep Kit 3.0. Briefly, First, use the Megaruptor instrument (Diagenode B06010001) to break the genomic DNAto a size of about 15 kb, then use beads to enrich and purify the target fragment DNA, and perform damage repair and end repair on the fragmented DNA. Finally, adapters were linked to both ends of the DNA fragments, and then the target fragments were selected using BluePippin (Sage Science, USA), which was the library to be tested.