GSM9789383: Vigna radiata T2 3h rep2; Vigna radiata; RNA-Seq
Total RNA was extracted from frozen leaf tissues using the FreeZol Reagent Kit (R711, Vazyme, China). RNA quality and concentration were assessed using a NanoDrop 2000 spectrophotometer (Thermo Scientific, USA) and agarose gel electrophoresis. Only high‑quality RNA was used for downstream library construction. Poly(A) mRNA was enriched using Oligo(dT) magnetic beads, fragmented, and reverse‑transcribed into double‑stranded cDNA. After end‑repair, A‑tailing, and ligation of MGI‑specific adapters, the cDNA libraries were amplified, purified, and circularized to form single‑stranded circular DNA (ssCir DNA), which served as templates for DNA nanoball (DNB) preparation. Sequencing was performed on the DNBSEQ platform.