GSM8488504: CAF-1 OFF 48 h Rep2 ATAC-seq; Mus musculus; ATAC-seq
To generate ATAC-seq libraries, two clonal replicates for used for each condition. 100,000 cells were used with the Active Motif ATAC-seq kit (#53150). Briefly, collected cells were washed in PBS, and lysed in ATAC Lysis Buffer. Nuclei were spun down and resuspended in the Tagmentation Master Mix containing assembled Tn5 transposomes. Transposase reactions were incubated at 37 °C for 30 min and then DNA from transposed nuclei was isolated and PCR-amplified using barcoded Nextera primers (Illumina). Library quality control and quantitation was carried out using high-sensitivity DNA Bioanalyzer or TapeStation and Qubit.