GSM9108861: 10 hours post fertilization; Nematostella vectensis; RNA-Seq
Whole embryos were dissociated with cysteine treatment (8 and 10hpf) or tripleE (12hpf).  Mechanical force was used to disrupt the tissues and obtain single-cell suspensions. Cell suspensions were washed with 0.1% BSA (bovine serum albumin), and cell viability and counts were assayed with a Cellometer X2 (Nexcelom). Cell suspensions were diluted to 1000 cells/ microliter and loaded into a 10x Genomics single cell platform using vs3 reagents (8 and 10hpf) or vs2 reagents (12hpf). 10X Genomics Chromium v2/v3 chemistry according to manufacturer protocols