GSM8830813: MMTV-PyMT Prrx1 f/f Tumors Homo Rep4; Mus musculus; RNA-Seq
Mammary gland carcinomas were collected from 14- to 15-week-old female PyMT mice. Tissues were minced and dissociated in digestion buffer (PBS, TH Liberase, DNase I) at 37°C for 45 minutes. Cell suspensions were filtered (70 µm, 40 µm), centrifuged, treated with ACK buffer, and resuspended in FACS buffer with EDTA. Viability was assessed using Trypan Blue, and cells were adjusted to 1,000 cells/µL for GEM preparation. Single-cell suspensions from ten mice (3 Prrx1 +/+, 3 Prrx1 +/f, 4 Prrx1 f/f) were processed using the Chromium Single Cell 3' Library & Gel Bead Kit v3.1 (10x Genomics) and sequenced on an Illumina NextSeq 2000 (100 bp read length).