ITS metabarcoding of soil sample
We used DNA metabarcoding to evaluate successional changes in soil fungal communities, comparing land managed for cattle, young, and older secondary forests at moist and dry sites in the Republic of Panama.  We used a 2 stage PCR protocol to prepare our libraries and the primer pair ITS1F (Gardes & Brun 1993) and ITS2R (White et al 1990), modified to include partial Illumina adaptors, was used to amplify the ITS1 region.  PCR 2 used primers containing remaining Illumina primers and an 8 bp index in both directions.  Products were cleaned an normalized with SequelPrep plates, pooled, and the  resulting library was concentrated using KAPAPure beads.  The library was sequencing with 2x250 bp reads on an Illumina MiSeq