GSM9026156: CUTRUN, Day7 Neuron, ctrl, MSL1-V5; Mus musculus; OTHER
Cells were fixed with 0.1% PFA for 10 minutes at RT, subsequently CUT&RUN was performed with minor modifications as published (Skene and Henikoff, 2017). Antibodies: MOF ( abcam; ab200660), FLAG (Sigma; F1804), V5 (Thermo Fisher; MA1-34099), H4K16ac (abcam ab109463), MSL2 (Sigma; HPA003413). Libraries were prepared using a NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, E7645)