XS11 penetration repeat 3
Total RNA was extracted from XS11 and Vsh1 after 3 days growth on cellophane using TRIzol Reagent (Invitrogen) with DNase I (TaKara) treatment. RNA quality was verified by agarose gel electrophoresis, Bioanalyzer (OD260/280 = 1.8-2.2, RIN  8.0), and NanoDrop quantification. High-quality RNA (>1g) was used to construct Illumina TruSeqTM libraries via polyA selection, cDNA synthesis (SuperScript kit), end-repair, and size selection (300 bp). PCR-amplified libraries were sequenced on Illumina NovaSeq 6000 (2150 bp). Raw reads were processed with fastp (https://github.com/OpenGene/fastp),60 aligned to the reference genome using HISAT2 (http://ccb.jhu.edu/software/hisat2/index.shtml),61 and assembled with StringTie (https://ccb.jhu.edu/software/stringtie/).62 Sequencing and library preparation were performed by Majorbio Bio-pharm (Shanghai).