GSM9317349: RELACS ChIP-Seq, Day7 Neuron,ctrl, H3K4me1, rep3; Mus musculus; ChIP-Seq
Cells were fixed with 1% PFA for 15 minutes at RT , Nuclei extraction, quantification and ChIP was performed using the published RELACS protocol (Arrigoni et al, 2018) Antibodies: H3K4me1 ( Diagenode; C15410194), H3K4me3 ( Diagenode; C15410003), H3K27me3 ( Diagenode; C15410195), H3K9me3 ( Diagenode; C15410193), , H3K27ac ( Diagenode; C15410196). Libraries were prepared using a NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, E7645)