GSM9828212: siYthdf3; Rattus norvegicus; RNA-Seq
RNA was harvested using Trizol regent. 1 ug of total RNA was used forthe construction of sequencing libraries For library preparation, the total RNA was used as input for RNA sequencing library preparation utilizing the KCTM Digital mRNA Library Prep Kit (Seqhealth Tech. Co., Ltd., Wuhan, China),according to the manufacturer's instructions. The kit eliminates duplication bias and errors in PCR and sequencing steps, by using unique molecular identifier (UID) of 12 random bases to label the cDNA molecules. The library preparation included the enrichment of PCR productscorresponding to fragments ranging from 200 to 500 base pairs. The enriched libraries were quantified and sequenced on a DNBSEQ-T7 platform (MGI) using the PE150 sequencing mode to generate paired-end reads.