CGL7_1-CHIP-seq (CRX1743154; CRA026198)
The method for obtaining cell nuclei in this experiment was the same as above, except that each replicate used 100,000 cell nuclei. After obtaining the cell nuclei, they were collected by centrifugation, and the nuclear buffer was replaced with wash buffer (Vazyme, TD904). Subsequent steps were performed according to the kit instructions, with special notes as follows: During primary antibody incubation, the experimental group received 2 uL of antibody and 48 uL of antibody buffer, while the control IgG group directly added 50 uL of antibody buffer (subsequent operations for the control group were the same). Each sample was spiked with 0.5 pg/uL spike-in for normalization.