Felis catus (gut metagenome)
Genomic DNA was extracted from the sample using a commercial kit (e.g., MagAtrract PowerSoil Pro Isolation Kit or FastPure Stool DNA Isolation Kit) following the manufacturer's instructions. DNA integrity, purity, and concentration were verified by 1% agarose gel electrophoresis, NanoDrop One, and Qubit 3.0 Fluorometer, respectively. The sequencing library was constructed with ALFA-SEQ DNA Library Prep Kit (or NEXTFLEX Rapid DNA-Seq Kit) by adapter ligation, PCR amplification, and bead-based purification. Library quality was assessed using Qubit 4.0 Fluorometer and QSEP400 High-throughput Nucleic Acid Protein Analysis System. Paired-end sequencing (150 bp read length) was performed on the DNBSEQ-T328 platform, generating raw sequencing data for subsequent bioinformatics analysis