GSM9038347: Subject_C20_Blood; Homo sapiens; RNA-Seq
Nasal tissue samples were minced, digested in a DNAse/Liberase solution for 30 minutes, strained and washed to create a single cell suspension. Paired whole blood was centrifuged layered on Histopaque 1077, with PBMC layer extracted and washed. Nasal and PBMC samples were loaded as single cell suspensions without sorting ScRNAseq gene expression libraries were contructed in accordance with the manufacturer protocol (Single cell 5' v2 protocol, 10x Genomics)