RNA-Seq of Paracoccidioides brasiliensis Pb18 wild type control - rep3
The Pb18 wild type and AsHapX transgenic strains were cultivated for a period of three days in BHI medium, after which they were incubated for ten minutes in liquid MVM medium containing the addition of 50 M BPS, to induce iron deficiency. Cells were centrifuged (800 g, 5 minutes, 4C) and RNA was extracted using the TRI Reagent (Sigma Aldrich) and the ZymoResearch Kit RNA Miniprep (California, USA), in biological triplicates. The cDNA libraries were prepared and sequenced on the Illumina NovaSeq6000 Sequencing System.