Shotgun metagenome of Lake Shobaidak: profundal water
DNA was extracted using the Qiagen Soil Pro Kit (Qiagen, Germany). The concentration and purity of the DNA were assessed using a Qubit 4.0 Fluorometer (Thermo Fisher Scientific, USA) and a Nanodrop-C-1000 spectrophotometer (Thermo Fisher Scientific, USA). The DNA was fragmented ultrasonically using a Covaris ME220 (Covaris, USA) to achieve fragments with an average size of 500 base pairs. Next-generation sequencing (NGS) libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, USA) and subsequently analyzed with a Bioanalyzer 2100 (Agilent Technologies, USA). The libraries were sequenced on a NovaSeq 6000 platform (Illumina, USA) to generate paired-end reads of 150 bp in length.