Metagenome of waste sludge with glycerol 5% (v/v) previously enriched with E. coli BW25113 as prey bacteria
Genomic DNA was extracted from waste sludge samples enriched with 5% (v/v) glycerol and Escherichia coli BW25113 after two day incubation. The bacterial community was analyzed by 16S rRNA gene amplicon sequencing. The 16S rRNA gene was amplified using universal primers targeting the bacterial 16S region (341f and 785r), and the resulting PCR products were sequenced using high-throughput sequencing