st2a
A purified PCR product was sequenced in the forward and/ or reverse directions on an Applied Biosystems 3130 automated DNA Sequencer (ABI, 3130, USA). Using a ready reaction Bigdye Terminator V3.1 cycle sequencing kit. (Perkin-Elmer/Applied Biosystems, Foster City, CA), with Cat. No. 4336817.  A BLAST analysis (Basic Local Alignment Search Tool) (Altschul et al., 1990), was initially performed to establish sequence identity to GenBank accessions. The sequence reaction was performed according to the the manufacturer instructions.