Plasmodium falciparum Dd2 sequencing
Plasmodium falciparum Dd2 sequencing
P. falciparum Dd2 cell culture was performed in Complete media composed of RPMI-1640 with Glutamine and 25 mM HEPES (Gibco), supplemented with100 Î¼M hypoxanthine (from c.c.pro), and gentamicin (Sigma-Aldrich) and supplementation of 10% human serum. Parasites were isolated by saponin treatment (0.01%) from 0.5mL of infected red blood cells at ~5% parasitemia. DNA isolation was perform using Qigan DNeasy blood and tissues kit.  Library preparation was done with the DNAseq_Library_Prep, TruSeq DNA PCR Free.