GSM8691270: cren784; Mus musculus; RNA-Seq
Total RNA was extracted from tibialis anterior (TA) muscle using Trizol reagent. Quality of RNA was assessed by Nanodrop and Bioanalyzer. The library was amplified and purified using AMPure beads. The library size and mass were evaluated and quantititative PCR was performed to validate the libraries. Barcoded samples were pooled equimolarly for simultanoeus sequencing. The quality of RNA-sequencing data was assessed for downstream analysis. Low-quality sequences and reads were removed.