GSM9379898: mm_atac_spreb_WT.17; Mus musculus; ATAC-seq
Cells were washed with PBS, then lysed with ATAC lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630). Resulting nuclei were then incubated with tagmentation enzyme (Illumina). Libraries from purified samples were made with the Nextera Indexing kit (Illumina).