GSM8724139: Adult; Mus musculus; RNA-Seq
Freshly collected neonate and adult tendon tissue from mice were flushed with ice-cold HBSSwo(Mg2+/Ca2+ free supplemented with 10mM HEPES, pH7.2) .The tissues were re-suspended in digestion buffers (HBSS containing Mg2 +Ca2+ supplemented with 2 ml collagenase type and 40 Uml DNase) and incubated at 37 ° C for 30 min. After washing, the cells were re-suspended in HBSS-/- with 0.04% w/v BSA. In brief, single cells are encapsulated with beads carrying cell barcode sequences in GEMs droplets. The GEMs droplets containing cells are collected, and within these droplets, the cells lyse and release mRNA. The mRNA binds to the cell barcode primers on the beads, completing the reverse transcription reaction. Subsequently, the GEMs are broken, and the cDNA is recovered and enriched through PCR amplification, followed by library construction of the cDNA. We used single cell RNA sequencing (scRNA-seg) to analyze the  diversity of tendon cells at different ages