GSM8831325: MMV085071_rep1; Plasmodium falciparum 3D7; RNA-Seq
Total RNA was extracted from the pellets with RNAzol (Sigma) according to the manufacture's protocol, quality checked with nanodrop before being snap frozen. The mRNA were enriched by NEBNext® Poly(A) mRNA Magnetic Isolation Module E7490 (New England Biolabs) for mRNA-Seq library construction.The cDNA library was prepared according to manufacturer's instructions using the NEBNext® Ultra™ II Directional RNA Library Prep Kit E7760 and NEBNext® Multiplex Oligos E7710 (New England Biolabs). The libraries were purified with Beckman SPRIselect beads (Beckman Coulter). The library quality was checked using Agilent Bioanalyzer. A total of 12 qualified samples were sent for sequencing (Novogene, Illumina NovaSeq 6000 system).