Gloeophyllum sp
DNA was extracted using the DNEasy Power Soil Kit (QIAGEN) according to manufacturers; in the case of rizosphere samples DNA extracts from five replicates of each treatment were combined to form composite genetic pools. DNA purity and concentration were measured using a NanoDrop spectrophotometer with the 260/280 ratio. Extracted DNA was submitted to MR.DNA (Shallowater,TX,USA) for amplification of the hypervariable V3-V4 regions of the 16S rRNA gene, this was  done through  bTEFAP (Bacterial TagEncoded FLX Amplicon Pyrosequencing) modified (Dowd et al; 2008)