GSM8317938: E0771_VEC; Mus musculus; RNA-Seq
Single-cell suspensions from tumor were collected. The cells were labeled with an antimouse-CD45-Percp/Cy5.5 antibody and a live/dead dye. FACS-sorted cells were gated on CD45+ live cells. Sorted cells from 5 mice were combined and were washed with PBS, and cell numbers and viabilities were assessed by trypan blue staining. The 10,000 CD45+ cells isolated from the lung were used for scRNA-seq library prep. The MUCIG pool plasmids were extratced by the Takara Maxi-prep kit. scRNA-seq library prep was  following the protocol from 10x Genomics Chromium Next GEM Single Cell 5' Reagent Kits V2. The sgRNA library readout was performed using readout PCR strategy