GSM9263233: DIOmouse_VHL_GAS_4; Mus musculus; RNA-Seq
Mice were dissected at the end of treatment and gastrocnemius muscles were frozen at -80 deg C before RNA extraction. Frozen tissues were homogenized with mortar and pestel in TRIzol reagent. RNA was extracted following TRIzol/chloroform protocol and precipited with isopropanol. The RNA pellet was washed with 75% EtOH solution and resuspended in nuclease-free water Libraries were prepared with 500 ng of RNA with a RIN value >7 using the Illumina stranded mRNA prep kit. Quality was assessed by TapeStation (280 bp fragments) and quantified using Qubit 1x dsDNA HS kit for sequencing by Illumina NextSeq1000 System