Chip-seq of Homo sapiens: intestinal epithelial cells
CUT&Tag (Cleavage Under Targets and Tagmentation) was performed to profile genome-wide binding sites of the glucocorticoid receptor (GR) in human cells. Briefly, cells were immobilized on concanavalin A-coated magnetic beads and incubated with a primary antibody against GR, followed by a secondary antibody to tether Protein A-Tn5 transposase fusion protein to the antibody-bound chromatin. Upon activation, the Protein A-Tn5 transposase inserted sequencing adapters at GR binding sites. After DNA purification, the tagmented fragments were PCR amplified to generate sequencing-ready libraries.