Metagenomic and Metabolomic Determinants of Immunotherapy Response in Non-Small Cell Lung Cancer
DNA was extracted from ~100 L of stool collected on Hemoccult II SENSA cards from 66 adults with NSCLC undergoing immune checkpoint inhibitor therapy. A single 50 L frozen DNA aliquot underwent wholemetagenome shotgun sequencing on the Illumina NovaSeq 6000 (pairedend 2  150 bp), yielding ~40 million reads per sample. Reads were quality filtered, assembled de novo using MegaHIT, and validated using known positive controls.