GSM9759760: Ctrl-3; Homo sapiens; RNA-Seq
Total RNA was extracted by RNAeasy RNA Isolation Kit (Beyotime Biotechnology) according to the manufacturer's instructions. mRNA is enriched with Capture Beads, fragmented at high temperature, reverse transcribed to first strand cDNA, followed by second strand synthesis with end repair and A tailing. Adapters are ligated, beads are used for purification and size selection, then PCR amplification and Illumina Novaseq X Plus sequencing .