Strand-specific total RNA-seq of conjugating E. vannus: 75 hours post-mixing
Strand-specific total RNA sequencing libraries were generated using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (Cat. E7420, NEB) following the manufacturers instructions. Prior to total RNA library preparation, rRNA depletion was attempted using TruSeq Stranded Total RNA Library Prep Gold Kit (Cat. 20020599, Illumina).