GSM9710127: CHOL_SEMA; Homo sapiens; RNA-Seq
Day-30 organoids were collected and pooled for each biological replicate. Samples were washed three times with ice-cold PBS and dissociated into single-cell suspensions by incubation with 0.5 mg/mL digestive enzyme I for 30 min at 37°C. Cell suspensions were filtered through a 70 μm cell strainer, centrifuged at 500 g for 5 min, and resuspended in PBS containing 0.01% BSA. Single-cell capture, barcoding, reverse transcription, and cDNA library construction were performed using the MGI DNBelab C-TaiM4 single-cell transcriptome platform according to the manufacturer's instructions. cDNA libraries were purified, amplified, ligated with sequencing adapters, quality-checked using an Agilent 2100 Bioanalyzer, and sequenced on the BGI DNBSEQ-T7 platform in paired-end 100 bp mode.