Genomic DNA Spraguea americanus spores
Illumina libraries were prepared using the Illumina DNA Prep (M) Tagmentation Kit (Cat#20060060, Illumina) and barcoded with Unique Dual Indexes - Set B (Cat# 20091656, Illumina) using the manufacturer's instructions. Illumina libraries were pooled and sequenced on P1 XLEAP-SBS 600-cycles flow cells, with PE 300 reads. The Illumina library was split over two sequencing runs to increase output.