GSM9310197: HCT116 cells,SON,4sU RNA rep2; Homo sapiens; RNA-Seq
After labeling, the RNA was extracted following NucleoSpin RNA kit (Macherey-Nagel). The biotinylated RNA was then purified by μMACS streptavidin MicroBeads (Miltenyi Biotec). Strand-specific libraries were generated using KAPA Stranded RNA-seq Library Preparation Kit with RiboErase HMR protocol (Roche)