GSM9468963: BIRC5-APEX2_L242F-EGFP, omit H2O2, post-enrichment, rep3; Homo sapiens; RNA-Seq
Following APEX2 labeling, cells were lysed by the addition of TRIzol reagent, and total RNAs were extracted according to the manufacturer's protocol. The extracted RNAs were then treated with DNase I at 37°C for 30 min to remove any contaminating genomic DNA, followed by purification using the RNA Clean & Concentrator kit. A 10 mL aliquot of purified RNA was reserved as the Input sample. The remained RNAs were subjected to affinity purification, and set as Enrich or Control samples. Input, Enrich, and Control samples from each biological replicate experiment were subjected to library construction by NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, E7770) with Poly(A) mRNA Magnetic Isolation Module (NEB, E7490) according to manufacturer's instructions.