Anaerobic sludge metagenome SAMN51366280
Metagenomic DNA was extracted from anaerobic sludge, fragmented to ~300 bp, and libraries were constructed using the NEBNext Ultra DNA Library Prep Kit (or specify actual kit used). Paired-end sequencing (150 bp read length) was performed on an Illumina NovaSeq 6000 (replace with your actual instrument model, e.g., HiSeq X Ten). Raw sequencing reads were quality-filtered and trimmed using fastp (v0.20.1) with parameters: -q 20 -l 30 -n 1. Host DNA sequences were removed by aligning the quality-controlled reads to host reference sequences using BWA (v0.7.9a) with default parameters, yielding high-quality non-host reads (raw data submitted to SRA).