GSM9079438: BAL, MHV1 infection, ZT6 time point, collected post-infection day 2, replicate 3; Mus musculus; RNA-Seq
To obtain BALs, mice were euthanized and their tracheas were cannulated with a 20G flexible catheter, 1-2 mm distal to the larynx.   The lungs were then slowly lavaged in 2 passes with a total of 1 ml sterile phosphate buffered saline (PBS). Whole lungs were manually excised and immersed in RNAlater solution Total RNA was isolated from lung homogenates using RNeasy Mini Kits (Qiagen) RNA from whole lung homogenates or BAL cell pellets were converted to cDNA libraries for sequencing using the the SMARTer kit following manufacturer protocols