GSM9060725: Bacteria,fermentation,rep1; Cellulomonas iranensis; RNA-Seq
Cellulomonas_iranensis were isolated from plant fermentations and made into single cell suspensions Library was performed according to the manufacter's instructions (MobiCube® High-throughput 3' Single Cell Transcriptome Kit).We uses droplet microfluidic technology to combine single cell suspension, reverse transcription reagent and Medtronic microspheres, which are encapsulated by oil droplets to separate individual cells in a single water-in-oil droplet.Cells in water-in-oil droplets are lysed, and the released mRNA is captured by the sequence on the microspheres, which links the cell tag to the cDNA product by a reverse transcription reaction. Next, water in oil was broken again, and cDNA was used as template for PCR amplification, quality inspection, sequencing library construction, sequencing, and data analysis.