GSM9241660: BCG-0.5_2; Mycobacterium; RNA-Seq
For in vitro samples: pellets from frozen cultures were processed with TRIzol reagent. Pellets were resuspended in TRIzol with one percent polyacryl carrier, transferred to screw cap tubes with zirconia or silica beads, and disrupted in a bead beater. Total RNA was isolated per manufacturer instructions and treated with DNase I, followed by TURBO DNase until PCR negative. For murine lung tissues: lungs were homogenized in TRIzol, centrifuged three thousand g for five minutes at four Celsius, and RNA purified as above non