GSM9760636: VCaP-injected tibia, ORX Balb/c nude mice, 10 weeks (Termination)_11.2_hs; Homo sapiens; RNA-Seq
Total mRNA was extracted from snap-frozen bones with the intratibial VCaP tumors from ORX and ORX+ADX mice and their corresponding contralateral non-tumor bearing control tibia (n=8 mice from each group, respectively) using TriZol reagent (Thermo Fisher Scientific) followed by RNeasy Mini Kit (Qiagen, Germany). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library.