GSM9789424: Vigna radiata T2 0h WGBS rep1; Vigna radiata; Bisulfite-Seq
Genomic DNA was extracted from frozen leaf tissues using the DNeasy Plant Maxi Kit (Qiagen, USA) following the manufacturer's protocol. DNA integrity and concentration were assessed using a NanoDrop spectrophotometer and agarose gel electrophoresis. Only samples with A260/280 ratios between 1.8 and 2.0 and high‑molecular‑weight bands were used for library construction. Whole‑genome bisulfite sequencing (WGBS) libraries were prepared using the TruSeq DNA Methylation Kit (Illumina, USA). Libraries were sequenced on the Illumina NovaSeq 6000 platform to generate 150 bp paired‑end reads. Library quality and insert size distribution were confirmed using a Bioanalyzer.