GSM9790999: Human ileal enteroid, HA-treated NEC, sample 3; Homo sapiens; RNA-Seq
Enteroids were removed from ECM domes using Organoid Recovery Solution and dissociated into a single-cell suspension using TryplE. Dead cells were removed from the suspension using a Dead Cell Removal (Annexin V) kit. Libraries were generated using the 10x Genomics Chromium GEM-X Single Cell 3' Gene Expression kit. Libraries were sequenced on an Illumina NextSeq 2000 instrument.