16S rRNA gene V4-V5 amplicon sequencing of seawater at station G4, 158 m
The V4-V5 region of the 16S rRNA gene was amplified using primers 515F-Y (5'-GTGYCAGCMGCCGCGGTAA-3') and 926R (5'-CCGYCAATTYMTTTRAGTTT-3'). Three technical PCR replicates were pooled and subjected to paired-end Illumina sequencing.