GSM8579847: ChIP_seq_KMS18-NRF1; Homo sapiens; ChIP-Seq
KMS27 and KMS18 cell lines were maintained in RPMI 1640 medium supplemented with 10% FBS and 1% glutamine. MM196 and MM217 cell lines were maintained in OPTI-MEM supplemented with 15% FBS and 1% glutamine. Subsequently, all cell lines were washed twice with 1x PBS. ChIP-seq libraries for NRF1 were prepared according to the ChIPmentation protocol (Christian Schmidl et al., Nature Methods, 2015) using Tn5 transposase.