GSM9060732: ADJIII-0019; Homo sapiens; OTHER
Briefly, five micron FFPE section were placed on histological slides (TOMO), deparafinized, stained with hematoxylin and eosin by the Research Pathology Platform at CRCL. After imaging, 6.5 x 6.5 mm areas were selected in the tissue section for each patient. After destaining and decrosslinking steps, human Visium probes were hybridized on the samples, ligated and transferred on the Visium capture slides using the CytAssist instrument (10X Genomics) Libraries were then prepared and sequenced on a NovaSeq 6000 sequencer (Illumina) targeting 50,000 reads/spot.