Amplicon analysis of enrichment cultures supplemented with laminarin
To enrich for deep-sea PVC bacteria, we established enrichment cultures using a basal medium as a control and the basal medium supplemented with 3.0 g/L laminarin. Specifically, we selected surface sediment samples from cold seep, hydrothermal vent, and seamount environments. These samples were cultured for one month at 28 C in these two distinct enrichment media. Each enrichment condition was performed in triplicate biological replicates. To elucidate the bacterial community structure within these enrichment cultures, 2 mL of each culture was harvested and subjected to amplicon sequencing analysis by Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai, China).