GSM8579472: CD45+, young, 3dpi; Mus musculus; RNA-Seq
CD45pos cells were isolated by FACS, from n.i. and injured SkM at 1, 3 and 5 dpi from yg (3 months) and old (25 months) female mice. Cells were isolated in two sessions using yg and old mice: n.i. and injured SkMs at 3 dpi in the first session, injured SkMs at 1 dpi and 5 dpi in the second session. For each condition, isolated CD45pos cells from two independent animals were pooled as one sample. After FACS, cell concentration and viability (80-90%) of each cell suspension were determined using 0.4% trypan blue (Gibco™: 15250061). Cells were washed and resuspended in 1x PBS (calcium and magnesium free) containing 0.04% BSA, to a final concentration of 1000 cells/µl. In brief, 20,000 CD45pos cells per condition were loaded onto a 10X Genomics Chromium platform Library was performed according to the manufacter's instructions (single cell 3' kit v3.1 protocol, 10X Genomics).