GSM8764078: AF16_L3_R2; Caenorhabditis briggsae; RNA-Seq
RNA was isolated using Trizol-reagent (Sigma, USA, Catalog Number T9424), chloroform, and isopropanol (Chomczynski and Sacchi 1987). cDNA libraries were constructed from 100–200 ng RNA using an Illumina-specific commercial kit (TruSeq RNA Sample Preparation Kit v2, Set A, Catalog number RS-122-2001).