RNA-Seq of Homo sapiens: CRYAB overexpression in EV-D68-infected RD cells
Total RNA was extracted from RD cells transfected with CRYAB plasmid following EV-D68 infection using TRIzol reagent. Total RNA was extracted from RD cells using TRIzol reagent according to the manufacturer's instructions. RNA integrity was assessed with an Agilent Bioanalyzer. Polyadenylated RNA [poly(A)+ RNA] was selectively enriched using oligo(dT) magnetic beads (library selection: other), then fragmented and reverse transcribed to cDNA. Libraries were prepared using the NEBNext Ultra RNA Library Prep Kit and sequenced on the Illumina HiSeq 4000 platform to generate 150 bp paired-end reads. Sequencing was performed by Shanghai Majorbio Bio-Pharm Technology Co., Ltd.