A. taxiformis tetrasporophytes cultured in seawater with their own exudates at 0.5g/L; replicate 2
Initially, RNA was isolated from the samples using RNeasy PowerPlant kit (Qiagen). The RNA quality was assessed using LabChip GX Touch Nucleic Acid analyser. Prior to library preparation, the sample underwent rRNA depletion using the RiboZero Plant kit. The prepared libraries were sequenced using Illumina NovaSeq 6000 to obtain 100bp single-end reads.