CAF_KO1 (CRX1825328; CRA027393)
Total RNA was extracted and poly(A)+ mRNA enriched using oligo(dT) beads. Following fragmentation, double-stranded cDNA was synthesized via random hexamer priming. After end repair and A-tailing, Illumina adapters were ligated. The library was size-selected using AMPure XP beads and quantified by Qubit/Bioanalyzer for sequencing.