GSM8997238: PA14 cells, SCFM-2 + citrate, biol rep 3; Pseudomonas aeruginosa; RNA-Seq
Total RNA was extracted using the NEB Monarch total RNA extraction kit according to the manufacturer's protocol for gram-negative bacteria. Ribosomal RNA was removed using the Ribo-Zero kit. Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero plus kit was used according to the manufacturer's protocol