GSM8968764: gtr1_2; Caenorhabditis elegans; RNA-Seq
RNA was extracted using the NucleoSpin® RNA kit (MACHEREY-NAGEL; 740955) according to the manufacturer instructions. mRNA libraries were prepared using KAPA Stranded mRNA kit with mRNA Capture Beads (KAPA Biosystems, KK8421). In brief, 1μg was used for the library construction; library was eluted in 20μL of elution buffer. All DNA sample libraries were pooled into 10 nM samples.