ITS seq of human fecal material: age 15 months
Barcoded Illumina sequence libraries were prepared from 2 l template of each sample and the Quick-ITS Plus NGS Library Prep Kit (Zymo Research; cat# D6424-PS2) as per manufacturers protocol. PCR amplification was performed on a QuantStudio6 Real-Time PCR System (ThermoFisher, cat# 4485691) as follows: 95C initial denaturation for 10 minutes; 42 cycles of 30 sec 95C denaturation, 30 sec 55C anneal, 3 minutes 72C extension.