GSM9107882: WT_CDK1as_H3K4me2_15min(biol rep1)_ChIP-Seq; Gallus gallus; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. After quenchng and cell lysis, chromatin was shear using Diagenode Biorupter. ChIP-seq libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® with NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1) according to the manufacturer's protocol. The library size distribution was analysed by Agilent Bioanalyzer with High Sensitivity DNA chip and submitted for Illumina deep sequencing (100 bp pair-end) at the Edinburgh Clinical Research Facility. Sequencing was performed on the NextSeq 2000 platform using the NextSeq 1000/2000 P2 Reagents (100 cycles).