D5_cKO7 (CRX1037647; CRA016210)
RNA of sorted NCSCs was extracted from the cell line using a QIAamp RNA Mini Kit, sheared to approximately 300-500 bp using a Covaris S220 instrument. Then the libraries were constructed through end-repair, A-tailing, adapter ligation and bisulfate-converted using a ZymoEZ RNA Methylation Kit