changpu_L1_A324 E1 (CRX1830036; CRA027574)
Formaldehyde crosslinking of leaf cells followed by chromatin fragmentation with DpnII restriction enzyme (for plant species). DNA ends were blunted, biotin-labeled, and proximity-ligated. Crosslinks were reversed, and DNA was purified and sheared to 350 bp fragments. Illumina-compatible adapters were ligated, and libraries were PCR-amplified with dual-indexed primers. Final library size selection was performed using AMPure XP beads. Sequencing was carried out on Illumina NovaSeq 6000 in PE150 mode.