Whole genome sequencing_PK2_1199
High-molecular weight DNA was extracted from thoracic muscle using proteinase K and CTAB digestion buffer, followed by chloroform: isoamyl alcohol extraction (24:1). DNA libraries were prepared using Illumina DNA Prep. 15x whole genome sequencing was carried out on a Novaseq 6000 S4 lane with 150-bp paired end reads.