Whole genome sequencing of single TDP43 positive cell PTA amplified DNA from homo sapien cerebellum
Single neuronal nuclei were isolated using FANS with co-staining of NeuN and TDP-43 antibodies from frozen brain (cerebellum). The nuclie were lysed and the genome amplifeid using primary template-directed amplification (PTA). Libraries were prepared using Kapa HyperPlus modified for PTA and sequenced to a target depth of 22X on an Illumina NovaSeq X Plus.