GSM9574756: MC38 cells, LIMCH1 knockout, replicate 2; Mus musculus; RNA-Seq
Total RNA was extracted from MC38 cells using TRIzol reagent according to the manufacturer's instructions. RNA integrity and concentration were assessed using an Agilent Bioanalyzer. RNA sequencing libraries were constructed using a poly(A) selection method to enrich mRNA. Libraries were prepared following the standard protocol recommended by BGI and sequenced on the DNBSEQ-T7 platform to generate paired-end reads.