GSM9758625: Mouse CD8+ TILs, WT anti-CTLA4; Mus musculus; RNA-Seq
Mouse tumor tissues were dissociated into single-cell suspensions. Tumor-infiltrating CD8-positive T cells were isolated and prepared for 10x Genomics Chromium single-cell RNA-seq library construction. Single-cell suspensions were processed using the 10x Genomics Chromium platform. Gel beads, cells, and reagents were loaded to generate GEMs; barcoded cDNA libraries were constructed according to the 10x Genomics single-cell gene expression protocol and sequenced on an Illumina NovaSeq 6000.