GSM9684822: SAL-sired_VTA_BIO REPLICATE 5; Rattus norvegicus; RNA-Seq
Brain tissue was dissected from adult (postnatal day 60+) rats: Immediately after euthanasia via decapitation, whole brains were removed and sectioned on a 1mM brain block. Bilateral NAc and VTA punches were quickly micro dissected & flash frozen on dry ice. Total RNA was isolated using a Qiazol/chloroform procedure and purified using the Qiagen RNeasy Micro kit. Sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit per manufacturer instructions