GSM9468562: Small RNAs from UAS-GFP-antisenseTirant driven by traffic jam Gal4 - replicate 2; Drosophila melanogaster; ncRNA-Seq
Argonaute-bound sRNAs were isolated using TraPR columns according to Grentzinger et al. (2020) Libraries were prepared by ligation of 3' barcoded adaptors containing 6 random nucleotides and a 5 nt in-line barcode and 5' adaptors containing 4 random nucleotides at the ends to reduce ligation biases followed by reverse transcription and amplification by PCR.