GSM8996200: Rat heart_HFpEF_rep5; Rattus norvegicus; RNA-Seq
RNA was extracted from the heart tissues using ISOGEN II (Nippon Gene Co., Ltd., Tokyo, Japan). Total RNA concentration was quantified using Dw-K2800 Nucleic Acid Analyzer (Shanghai Drawell Scientific Instrument Co., Ltd., Shanghai, China). Total RNA (800 ng) from each heart sample was converted to Illumina compatible libraries using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB) according to the manufacturer's protocol.