GSM9760854: HCT116 cells, si-NC2; Homo sapiens; RNA-Seq
Total RNA was isolated by TRIzol Reagent. 1 μg total RNA was used for mRNA sequencing library preparation. poly(A) mRNA isolation was performed using Oligo(dT) beads. mRNA sequencing libraries were made using the NEBNext Ultra II Directional RNA Kit.