GSM9825292: HepG2 siNAT10 knockdown replicate 3; Homo sapiens; RNA-Seq
Total RNA was extracted using TRI reagent (Sigma-Aldrich) according to the manufacturer's instructions. RNA quantity and quality were assessed by spectrophotometry. 3' mRNA-seq libraries were prepared using the Lexogen QuantSeq 3' mRNA-Seq Library Prep Kit REV according to the manufacturer's protocol. Libraries were sequenced on Illumina NovaSeq 6000 (paired-end, 150 bp).