GSM8997131: C57BL/6J/mice, nTG, Sed, M, 5; Mus musculus; RNA-Seq
Total RNA was extracted from 7-9 samples per group for 64 samples total using the RNeasy Fibrous Tissue Mini Kit (Qiagen 74704) according to the manufacturer's protocol. Complementary DNA (cDNA) was synthesized via reverse transcription using Omniscript RT Kit (Qiagen 205111) according to the manufacturer's protocol. Libraries were prepared using the NEBNext rRNA Depletion Kit (Human/Mouse/Rat) v2 and the xGen Broad-range RNA Library Prep according to the manufacturer's protocol.