DNA-seq of silk moth: larva female gut
The gut was dissected from a single female last instar larva of D. sibiricus, washed from gut content, and frozen in liquid nitrogen. High-quality genomic DNA was extracted using MagAttract HMW DNAKit 48 (Qiagen, USA) with added RNase A according to the manufacturer's protocol. The HiFi SMRTbell library was constructed using the SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences, CA, USA) following the manufacturers instructions. The library was sequenced on the PacBio Sequel II system (Pacific Biosciences), using 1 SMRT Cell 8M, Sequel II binding Kit 2.0, and Sequel II Sequencing Kit 2.0 (Pacific Biosciences). Consensus HiFi reads were generated using CCS v.6.4.0 (Pacific Biosciences) software with enabled "--hifi-kinetics" parameter.