GSM9315940: Healthy Donor 2 - ILC2 TrkA-; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNEasy Micro Plus Kit (Qiagen) according to the manufacturer's instructions. Cells were lysed in RLT Plus buffer containing b-mercaptoethanol and genomic DNA was removed using a gDNA Eliminator spin column. RNA was purified via silica membrane spin columns with DNase digestion, washed, and eluted in RNase-free water. Total RNA was quantified with a Qubit (fluorimeter from Life Technologies) and RNA integrity assessed with a Bioanalyzer (Agilent Technologies). The SMART-Seq mRNA kit from Takara was used for the reverse transcription and cDNA amplification according to manufacturer's specifications, starting with 2 ng of total RNA as input. 200 pg of cDNA were used for library preparation using the Nextera XT kit from Illumina.