GSM9314938: hCOs, SOBP 7Gy, rep2; Homo sapiens; RNA-Seq
RNA was isolated from hCOs 48 hours after irradiation using the RNeasy Lipid Tissue Mini Kit (Qiagen, 74804) following the manufacture's instruction. Libraries were generated using the Lexogen QuantSeq 3' mRNA-seq Library Prep Kit (Lexogen, 015.96), with 200 ng of RNA used as input for each sample. All libraries were pooled in equimolar concentrations, and a 1.8 pM superpool with a 15% PhiX spike-in was sequenced on a NextSeq 500 using 75 bp single reads.