GSM9710202: Co-culture, Female, 29; Homo sapiens; RNA-Seq
Constructs for RNA seq were harvested (n = 3 per group) and immediately frozen at −80°C in TRIzol reagent (Thermo Fisher Scientific, United States, #15596018). Total RNA was isolated using PureSPIN mini spin columns (Luna Nanotech, Canada, #NK051-250) according to the manufacturer's instructions. The remaining isolated RNA was sequenced at the University of British Columbia Biomedical Research Centre using an Illumina NextSeq 2000 platform with paired-end 59 bp × 59 bp reads. FASTQ files were analyzed using Partek Flow (version 12.9.0). Reads were aligned to the human genome using STAR (v2.7.8a), and transcript quantification was performed using the Partek E/M algorithm with the hg38 Ensembl transcript annotation model (release 113_v2).