sn-RNAseq of cells from wide-type embryonic hearts and Emp2-/- ones
sn-RNAseq of cells from wide-type embryonic hearts and Emp2-/- ones
E10.5 embryonic hearts was dissected and quickly frozen in liquid nitrogen to retain intact nuclei.  After nuclei extraction and purification, approximately 15,000 nuclei per sample were loaded on a Chromium Controller (10x Genomics) to generate gel bead-in-emulsions.  The sc-RNA-seq libraries were generated using Chromium Single Cell 3â€™ Reagent Kits v2 and quantified using a Bioanalyzer 2200.