16S amplicon seq of EMR2 roots Gd03
DNA was extracted from surface disinfected roots with the Invisorb Spin Plant Mini Kit. 335F/769R 16S rRNA primer pair was used for PCR. Purified PCR products were indexed with the Nextera XT Index Kit v2 Set A and B. Library sequencing was performed using the MiSeq Reagent Kit v3.