GSM8515272: Nontargeting_control_rep3; Homo sapiens; RNA-Seq
Total RNA was extracted using the Promega Maxwell RSC simplyRNA tissue kit, cat# AS1340. RNA-Seq libraries were prepared using the Illumina TruSeq Stranded mRNA library prep kit, cat# 20020595 with the IDT for Illumina TruSeq RNA UD Index set, cat# 20040871, following the manufacturers protocol and using 1ug of RNA for each sample. Finally, samples were pooled in an equimolar fashion and sequenced on an Illumina NovaSeq 6000 S4 flow cell, generating 100 base-pair paired end reads.