GSM9241634: NFIL3-ChIP, Runx3 R122C/R122C, Rep.2; Mus musculus; ChIP-Seq
In vitro activated CTLs transduced with 6xHis-tagged NFIL3 were cross-linked with DSG and formaldehyde. Nuclei were isolated from the cells using lysis buffer and chromatin DNA was fragmented by sonication. The lysate was incubated with anti-NFIL3 microbeads (MBL, D291-11) overnight at 4 degree C. Chromatin DNA was eluted from Dynabeads and reverse-crosslinked. Samples were treated with RNase A and Proteinase K, and DNA was purified using ChIP DNA Clean and Concentration Kit (Zymo Research). Purified DNA was used for library preparation with NEBNext Ultra II Kit and NEBNext Multiplex Oligos for Illumina . Sequencing was performed by Macrogen Japan with Illumina NovaSeq X Plus.