GSM9758582: scSeq night; Danio rerio; RNA-Seq
Pineal glands were dissected and enzymatically dissociated in papain to produce isolated cells. This single-cell suspension was then loaded onto a commercially available droplet-based 10x Chromium system (v3 platform, 10x genomics). Libraries were constructed using 10x Genomics' Single Cell 3' Reagent Kit v3 according to the manufacturer's instructions