GSM9063508: ctrl_6dpf; Danio rerio; RNA-Seq
The isolated otic vesicle and inner ear organoid tissue was cut into 2–3 mm sections and rinsed with Hanks' Balanced Salt Solution (HBSS). Enzymatic digestion was performed for 15 minutes using a tissue dissociation solution (Singleron Biotechnologies, Nanjing, China). The resulting cell suspension was filtered through a sterile 40-μm filter (Corning, NY, USA) and centrifuged at 500 g for 5 minutes. Erythrocytes were removed with a lysis reagent (Singleron Biotechnologies, Nanjing, China) for 10 minutes. The cells were resuspended in phosphate-buffered saline (PBS) and stained with trypan blue (T6146, Sigma, Burlington, VT, USA). Cell viability was assessed using a TC20 automated cell counter (Bio-Rad, Hercules, CA, USA). ScRNA-seq libraries were prepared using the GEXSCOPE® Single Cell RNA Library Kit (Singleron Biotechnologies).