RNA-seq of Kaiso deficient HEK293 cells
Total RNA was extracted with Trisol reagent and PureLink RNA micro Kit (Invitrogen) according to manufacture instruction. Quality was checked with BioAnalyser and RNA 6000 Nano Kit(Agilent). PolyA RNA was purified with Dynabeads mRNA Purification Kit (Ambion). Illumina library was made from polyA NEBNext Ultra II RNA Library Prep (NEB) according to manual. Sequencing was performed on NextSeq2000 with 50 bp read length. At least 10 millions of reads were generated for each sample.