mRNA-seq of conjugating E. vannus: 75 hours post mixing
Briefly, the mRNA sequencing libraries were generated using NEBNext Ultra RNA library prep kit for Illumina (Cat. E7530, NEB) following the manufacturers instructions. Prior to mRNA library preparation, poly (A)+ RNA was isolated using magnetic beads coupling of Oligo d (T) (Cat. N401-01/02, VAHTS mRNA Capture Beads, Vazyme, China).