GSM9025926: CUT&TAG H3K27ac 48hr post lenti infection with GATA2 coding sequence in TF1 cells; Homo sapiens; OTHER
After tagmentation, DNA was isolated with the use of the Monarch PCR & DNA clean up kit (Cat no: T1030L, NEB) by resuspending 50 uL of each reaction with 700 uL of DNA Binding Buffer and following the manufacturer's recommendation thereafter. DNA was eluted in 22 uL of elution buffer 21 ul of DNA were mixed with 25 uL of 2X NEBNext HiFi PCR Master mix (NEB), 2 uL of 10 uM P5 primer, 2 uL of 10 uM indexed P7 primer. 50 uL of amplified DNA were cleaned up with 1.2X AMPure XP beads as per manufacturer's recommendation. DNA was quantified with Qubit fluorometer. Indexed libraries were pool proportionally and sequence with 150-bp paired end in a NexSeq 500 to achieve at least 5 million reads per sample.