RNA-Seq of rat: lung
For physiological saline group replication 3, rats were anesthetized and fixed in a supine position, a rat-specific laryngoscope was inserted, and 50 l of pre-prepared physiological saline was slowly injected. The rats were exposed every two days for a total of 12 exposures. At the end of the experiment, the lungs of rats were removed for miRNA transcriptome analysis.