RNA sequencing of P. falciparum treated with CQ
Total RNA was extracted following the Ponts, Chung, and Le Roch (2012) protocol. Parasite cultures were thawed, centrifuged, and treated with pre-warmed TRIzol for deproteinization. RNA was extracted using chloroform and precipitated with isopropanol, followed by DNase treatment. RNA concentration was measured using a NanoDrop spectrophotometer, and quality was reassessed before sequencing. Human rRNA was depleted using the MGIEasy rRNA depletion kit, and RNA libraries were prepared using the MGIEasy RNA Library Prep Set. Sequencing was performed at the Agricultural Research Council (South Africa) using the Illumina MiSeq system.