RNA-Seq of Mus musculus: brain tissue from CA model
Total RNA was extracted from mouse brain tissues using TRIzol reagent according to the manufacturers instructions. RNA quality and integrity were assessed using agarose gel electrophoresis and an Agilent 2100 Bioanalyzer. mRNA was enriched using poly(A) selection and subsequently fragmented into short fragments. First-strand cDNA was synthesized using random hexamer primers, followed by second-strand cDNA synthesis. The cDNA fragments were end-repaired, A-tailed, and ligated to sequencing adapters. The libraries were amplified by PCR and purified. Library quality was assessed using the Agilent 2100 Bioanalyzer. Sequencing was performed on an Illumina NovaSeq 6000 platform with paired-end reads.