Whole metagenome
Metagenomic  DNA   extraction   from   the   ISR   batch   experiment   samples   were performed   using   the   Nucleospin   soil  ,   following   the   manufacturer's protocol. Whole metagenomic library was prepared using NEBNext UltraTM II FS DNA Library Prep Kit for Illumina (Cat. Nr. E7645S, New England Biolabs) with NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs, Cat. Nr. 6440S, New England Biolabs) as per manufactures instruction. The quantity and fragment sizes of all libraries were assessed by capillary electrophoresis on a Agilent Tapestation 2100 (Agilent) using D1000 Screen Tapes. The pool prepared for the libraries consisted of 8 equimolar libraries and sequenced on Novaseq 6000 Platform (Illumina, San Diego, CA, USA) with 150 bp paired end reads.