GSM9899947: Stomach,inflamed_type,LNQ; Homo sapiens; RNA-Seq
Human GC tissues were washed with PBS, cleared of blood and adjacent non-tumor tissue, minced, and enzymatically dissociated into single-cell suspensions. Total RNA was extracted from single cells using the 10x Genomics Chromium Single Cell 3' Reagent Kit v3 according to the manufacturer's instructions. Single-cell RNA-seq libraries were prepared using the 10x Genomics Chromium Single Cell 3' Reagent Kit v3 according to the manufacturer's instructions. Briefly, single-cell suspensions were loaded onto the 10x Genomics Chromium platform for cell capture and barcoding, followed by reverse transcription, cDNA amplification, and library construction.