Cold acclimation of strong cold tolerant cultivar
Method: Healthy and evenly grown fourth-leaf seedlings were placed in an incubator for CA at 40.5C for 7 days, with a 1C decline in temperature per hour until 4C was reached. After 7 days of CA, and non-acclimatized or cold-shocked (CS) seedlings were transferred to a freezing chamber for four hours of sub-zero temperature treatments at 0C, -3C, and -7C, with 1C reduction in temperature per hour until -7C. To comprehend the molecular regulatory mechanism of genes following CA, the 4th leaves of the subzero temperature (0C, -3C, and -7C) treated seedlings of both cultivars were collected. Three biological repeats were used for transcriptomics. Model: Key state laboratory of arid land crop science utilized Complete block design.