RNA-seq of Glycin max: SYMMV at 25
Total RNA was extracted from the plant leaves using RNeasy Plant Mini Kit (Qiagen, Germany), and ribosomal RNA was removed before constructing the RNA-sequencing libraries. Total RNA with an RNA Integrity Number (RIN) value greater than or equal to 7 was used for library preparation. RNA sequencing libraries were prepared using the TruSeq Stranded mRNA LT Sample Prep Kit according to the manufacturers instructions (Illumina, San Diego, CA, USA). The libraries, paired with their respective indices, were sequenced using the Illumina NovaSeq 6000 system (Illumina).