GSM9063711: donor 7, female, primary human macrophages control; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNAqueous Micro Kit (invitrogen) according to the manufacturer's protocol but heating the Elution Solution to 65°C. mRNA was extracted using poly-T oligo-attached magnetic beads, then fragmented. cDNA was quantified using real-time PCR and fragment size was measured using a Bioanalyzer. The cDNA library was then constructed and analyzed with Qubit. Quantified libraries were then pooled and sequenced on Illumina platforms, based on sequencing by synthesis.