GSM9315988: Unstim_PM_Neuts_002; Homo sapiens; RNA-Seq
Total RNA was extracted from 1.5 million cells using Direct-zol RNA Miniprep Kit (Zymo Research). Libraries were prepared using the NEB Ultra II Directional RNA Library Prep Kit for Illumina with the NEBNext Poly(A) mRNA Magnetic Isolation Module (supplier info). 20 ng total RNA was used for each sample and libraries underwent 16 cycles of PCR amplification. Agilent Universal Human Reference RNA was prepared as a process control. Libraries were sequenced on the Novaseq 6000 using a 50x50 run configuration at a targeted depth of 25M (50M paired-end) reads. RNA-seq 50 x 50 Paired-end sequencing reads