Illumina NovaSeq 6000 paired end sequencing
Sequencing libraries were prepared from 1μg DNA using the TruSeq PCRfree DNA sample preparation kit (cat# 20015962, Illumina Inc.) and unique dual indexes (cat#20022370, Illumina Inc.), targeting an insert size of 350bp. The library preparation was performed according to the manufacturers' instructions (guide#1000000039279).