Sequel II sequencing of SAMD00901851
Genomic DNA was first sheared using a g-TUBE (Covaris) to generate appropriately sized DNA fragments. Library preparation was carried out using the SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences), which includes steps for DNA damage repair, end-repair, and ligation of SMRTbell adapters. To enrich for longer DNA fragments, a size-selection step was performed using the BluePippin system (Sage Science), with a cutoff set to remove fragments smaller than 40 kbp. The prepared libraries were subsequently bound to polymerase using the Sequel II Binding Kit 2.0 and sequenced on a Sequel II system with the Sequel II Sequencing Kit 2.0 (Pacific Biosciences).