GSM9574410: ATDC5 Day 21 rep4; Mus musculus; RNA-Seq
RNA was extracted with Qiagen Rneasy Fibrous Tissue Kit according to manufacturers instruction Novogene in house protocol: Purification: mRNA was isolated from total RNA using poly‑T oligo‑attached magnetic beads. cDNA synthesis: Fragmented mRNA was reverse transcribed using random hexamer primers to generate first-strand cDNA, followed by second-strand synthesis. Library construction: Libraries were prepared by end repair, A‑tailing, adapter ligation, size selection, amplification, and purification. Quality control: Libraries were quantified using Qubit and qPCR; size distribution was assessed with a Bioanalyzer.