RNA-Seq of Homo sapiens: RBM26 and CRYAB rescue after siRNA knockdown in EV-D68-infected RD cells
RD cells were subjected to RBM26 siRNA knockdown followed by re-expression of both RBM26 and CRYAB after EV-D68 infection. Total RNA was extracted from RD cells using TRIzol reagent according to the manufacturer's instructions. RNA integrity was assessed with an Agilent Bioanalyzer. Polyadenylated RNA [poly(A)+ RNA] was selectively enriched using oligo(dT) magnetic beads (library selection: other), then fragmented and reverse transcribed to cDNA. Libraries were prepared using the NEBNext Ultra RNA Library Prep Kit and sequenced on the Illumina HiSeq 4000 platform to generate 150 bp paired-end reads. Sequencing was performed by Shanghai Majorbio Bio-Pharm Technology Co., Ltd.