GSM9319588: Mus musculus, splenocytes, 18-24 months, Female, Sepsis (CLP+7dDCS), id: S-082923-OF1; Mus musculus; RNA-Seq
Splenocytes were obtained and counts were quantified using Cellometer™ Auto 2000 Cell Viability Counter (Nexcelom Bioscience, Lawrence, MA). A million cell concentrations with a minimum of 85% cell viability were obtained for scRNASeq using Chromium X instrument (10X Genomics, Pleasonton, CA). Libraries were prepared with the 10X's Chromium Next GEM Single Cell 5' v2 kit profiling 10,000 cells per sample. To ensure quality during library preparation RNA integrity and dsDNA insert were assessed using the Tapestation 4200 (Agilent, Santa Clara, CA) and Qubit 4 (Invitrogen, Carlsbad, CA). Final concentration of the library pool was measured with the NEBNext Library Quant Kit (New England Biolabs, Ipswich, MA). Libraries were sequenced at a minimum depth of 20,000 read pairs per cell on an Illumina Novaseq X plus Platform (Illumina, San Diego, CA).