GSM9380382: HUDEP2 (sgEGR1), PBS Treated - R1; Homo sapiens; ATAC-seq
DNA was isolated from 50,000 cells and resuspended in cell lysis buffer. DNA purification was done using the Qiagen MinElute Reaction Cleanup Kit and adapters were added to Tn5 treated samples for library preparation by using the kit NEBNext Ultra II Q5 2× Master Mix (NEB). Amplification of the library prepared samples was done by PCR and cycles determined by qPCR with the help of NEBNext Library Quant Kit (NEB).