Responder to CTLA4/PD1
The following populations were then purified by sorting: P1: GFP-CD45+CD3+ (T cells), P2: GFP-CD45+CD3- (non-T immune cells), P3: GFP+/-CD45-CD3- (tumor and stromal cells). P1, P2 and P3 for each sample were then mixed back together at a 2:1:1 ratio, respectively. 5000 cells from each of the mixed sorted samples for each condition were loaded onto 10x Genomics Chromium System