GSM8764225: S2D2, mDPCs from Dmp1-Cre; Setd2fl/fl mice; Mus musculus; RNA-Seq
The mDPCs were isolated from control mice and Dmp1-Cre;Setd2fl/fl mice at P6. Total RNAs were extracted from mDPCs using Trizol reagent (Takara) and then subjected to PE150 Hiseq, which was performed by Anoroad in Wuhan. Each sample contained pooled RNAs from 3 biological replicates and was mixed with an equal mass of RNAs to minimize variation across samples. After the samples were qualified, 1~3 μg total RNAs of each sample was used as the starting material to construct a transcriptome sequencing library. > Library examination  RNA concentration of library was measured using QubitR RNA Assay Kit in  Qubit® 3.0 to preliminary quantify and then dilute to 1ng/ul. Insert size was assessed  using the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA), and  qualified insert size was accurate quantification using StepOnePlusTM Real-Time PCR  System (Library valid concentration> 10 nM).  > Library clustering and sequencing  The clustering of the index-coded samples was performed on a cBot cluster  generation system using HiSeq PE Cluster Kit v4-cBot-HS (Illumina) according to the  manufacturer's instructions. After cluster generation, the libraries were sequenced on  an Illumina platform and 150 bp paired-end reads were generated.