GSM9473656: MASH-3; Sus scrofa; RNA-Seq
Liver samples were obtained through surgical biopsy, preserved in SCelLiVe® Tissue Storage Solution, and minced into small pieces. The tissue was digested at 37°C for 40 minutes using a digestion solution containing 1 mg/ml Collagenase I, 1 mg/ml Collagenase II, 1 mg/ml Dispase II, and 10 U/ml DNase I. After obtaining the cell suspension, red blood cells were removed, and the sample was centrifuged at 50 g for 2 minutes to isolate non-parenchymal cells (NPCs). Library was performed according to the manufacter's instruction(GEXSCOPE® Single Cell RNA Library Kit)