="16S rRNA gene sequencing of bacterial and archaeal community from rumen sample "&A76
Bacterial and archaeal partial 16S rRNA genes were amplified from rumen fluid samples using primer sets targeting the bacterial V1V3 (FAM27f/Bac515R) and archaeal V6V8 (AR934F/Arc1386R) regions. Amplicons were pooled and sequenced using Illumina MiSeq.