GSM9168513: Pt 'AML1', CD34-enriched BMA, whole RNA 10x capture (3-prime); Homo sapiens; RNA-Seq
Density-gradient collected bone marrow mononuclear cells were magnetically enriched for cells with CD34 cell surface markers (Miltenyi Biotec #130-097-047 or Stemcell #17856) or CD117 markers (Miltenyi Biotec #130-091-332). Both unsorted and enriched cells per each patient/timepoint were stained with BioLegend TotalSeq-C DNA-barcoded antibodies (for ADT expression). Single cell whole RNA capture and DNA-barcoded cell surface proteins capture (when applicable) were performed using either the 10x Genomics Next GEM Single Cell 3' v3.1 with Feature Barcode chemistry or the 10x Genomics Next GEM Single Cell 5' v2 with Feature Barcode chemistry according to manufacturer's protocols (10x Genomics User Guide document #CG000317_Rev_B for the 3' chemistry; #CG000330_Rev_A for the 5' chemistry). scRNA-seq