GSM9741531: Pooled GEX library, pulmonary myeloid cells, mixed bone marrow chimeras infected with C. neoformans gcs1 mutant; Mus musculus; RNA-Seq
Single cell suspension was prepared from lung tissue. Cells were stained with hashtag antibodies (TotalSeq-C0301 to C315, BioLegend #155861-#155889) and myeloid cells (Live/CD45+/IV-/B220-/CD90.2-/Ly-6G-/MHCII+/CD11c+/CD64+) were sorted and pooled for library preparation. Library was prepared using Chromium Next GEM Single Cell 3' Reagent Kits v3 (10x Genomics, document number: CG000220 Rev F) across 5 lanes. GEX and HTO libraries were sequenced separately.