ITS amplicon sequences from Phragmites australis-associated metagenome: top-litter
The ITS1 region was amplified with primers ITS1-F/ITS2 (Gardes & Bruns, 1993; White et al., 1990) using metagenomic DNA extracted from the indicated plant tissues. PCR products were purified with AMPure XP beads (Beckman Coulter), indexed with Nextera XT dual indices (Illumina), and cleaned again with AMPure XP. Libraries were sequenced on an Illumina NextSeq 2000 (P1 300cycle kit; 2 x 150 bp) with a 30% PhiX spikein at the Louisiana State University Cancer Research Center Translational Genomics Core.