16S rRNA of Wombat Control: feces
Faecal and soil samples were sampled on days 0, 1, 2, 3, 5, 8, and 12. For soil, the top 10 cm of soil were sampled following method Bolland (1992) and stored in -80 C prior to DNA extraction. For each of the samples, a target of 0.25 g of faecal or soil samples were transferred to a collection tube for DNA extraction. Faecal samples were extracted following protocols of the DNeasy PowerFaecal extraction kit (QIAGEN). Where DNA weight was <0.25g a smaller weight was used and measured. Subsequently, the V3-4 region of the 16S rRNA amplicon genes were amplified prior to sequencing process, as described in Henry et al. (2016). 16S rRNA amplicon sequencing was then conducted using Illumina MiSeq as per the manufacturers instructions, under previously optimised settings