GSM9825493: CD8+ T cell transduced with empty vector replicate 1; Mus musculus; RNA-Seq
T cells were collected and centrifuged at 750 × g for 5 minutes. Cells were lysed with DNA/RNA Shield (Zymo Research, #1100-50) at a density of 1 × 10⁶ cells per 50 µL. Lysed samples were submitted to Plasmidsaurus for library construction and sequencing. Library construction and RNA-seq sequencing (10 million reads/sample) were performed by Plasmidsaurus. In brief, they converted mRNA into complementary DNA (cDNA) via reverse transcription and second-strand synthesis, followed by tagmentation and library indexing and amplification.