GSM9380084: Dissociated granuloma tissue from *AB (WT) zebrafish; Danio rerio; RNA-Seq
Granulomas were extracted from infected zebrafish and transferred to a 1.5 mL tube. Cells were dissociated using 1 mL of 0.05% trypsin/EDTA (ThermoFisher Scientific, 25300054) at 30 C for 35 minutes with rocking. Cells were then collected by centrifugation for 5 min at 300 xg, resuspended in 1x DPBS with 10% FBS on ice, and passed through a 40 mm strainer. Single-cell suspenions were submitted to the Molecular Genomics Core at Duke Molecular Physiology Institute for library preparation using Chromium GEM-X 3' chemistry on the 10X Genomics platform. Libraries were sequenced at the Duke Center for Genomic and Computational Biology.