RNA-Seq of mus musculus: sexually immature female ovaries
Pandora-seq (Panoramic RNA Display by Overcoming RNA modification Aborted sequencing) addresses the issue of adapter ligation caused by terminal modifications in small RNAs (ranging from 15-50 nt) through enzymatic treatments (T4PNK, AlkB). This method converts 3' phosphate groups or 2',3' cyclic phosphate groups into 3'-OH groups, and 5'-OH groups into 5' phosphate groups. Additionally, it removes specific RNA methyl modifications (m1A, m1G, m3C, m22G), thereby overcoming the obstruction that these modifications impose on reverse transcriptase activity.