RNAseq of mus musculus Prostate PDX primary site
Total RNA was extracted from primary xenograft tumors and matched prostate metastatic lesions using TRIzol Reagent (Invitrogen). RNA quality was evaluated using an Agilent 2100 Bioanalyzer. Sequencing libraries were prepared using the Illumina TruSeq Stranded mRNA Library Preparation Kit and sequenced on an Illumina NovaSeq 6000 platform to generate 154-bp paired-end reads.