GSM9024045: iMGL-iNSpheroids, iPSC DF6-9-9T.B cell line, control 0h; Homo sapiens; RNA-Seq
Untransduced and rAAV9-mCherry transduced iMGL-iNSpheroids (derived from the iPSC(IMR90)-4 and iPS DF6-9-9T.B cell lines) were analysed by single nuclei RNA-sequencing (sn-RNAseq). Samples were collected at 0-, 6- and 24-hours post-transduction, and sedimented by centrifugation at 300 xg, for 5 min. Cells were then resuspended and cryopreserved in cell freezing medium, CryoStor CS10 (STEMCELL Technologies, cat. no. 100-1061) and stored at -80 °C until shipment. Samples were sent for nuclei isolation and sequencing at Genewiz laboratories (Leipzig, Germany). Raw data from snRNAseq was analysed and processed into a transcript count matrix using Cell Ranger (https://www.10xgenomics.com/, v6.1.1) from the Chromium Single Cell Software Suite by 10x Genomics. Fastq files were generated using the Cell Ranger mkfastq command with default parameters. Gene counts for each cell were quantified with the Cell Ranger count command with default parameters. For all analyses, the human (GRCh38) plus the mCherry genes were used as reference.