Bactrocera zonata short read WGS (female)
The short read whole genome sequencing library was constructed using the NxSeq AmpFREE Low DNA ligation kit. The library was sequenced on the MGI technologies DNB-SEQ G400 using PE150 cycles. Before sequencing the library was first circularized using the MGI library conversion kit (APPA) followed by rolling circle amplification (RCA) to form the DNA nanoballs. The DNA nanoballs are then loaded in the flowcell before sequencing.