GSM9380435: YCC-32; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNeasy Mini Kit (Qiagen), quality-assessed with NanoDrop and Bioanalyzer. Total RNA was extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer's protocol. RNA integrity was assessed using the Agilent 2100 Bioanalyzer. Libraries were prepared using the Illumina TruSeq Stranded mRNA Library Prep Kit, including poly(A) mRNA enrichment, fragmentation, cDNA synthesis, adaptor ligation, and PCR amplification. Final libraries were quantified, pooled, and sequenced on an Illumina HiSeq 2500 platform (2 x 100 bp).