GSM8488426: osteochondral fusion; Homo sapiens; RNA-Seq
To collect enough cells for single-cell RNA sequencing, three osteochondral fusions were pooled together and were dissociated to single cells by Liberase after cut into small fragments. Digestion was stopped until no large fragments could be found and the cells were filtered by 70μm strainers. After that, the cells were treated with hemolysis reagents for 10 min at RT. Then 1 mL basal medium composed of DMEM/F12, 10% FBS, 1% pen-strep was added and the cells were centrifuged at 300g for 5 min and were resuspended in the medium mentioned above for subsequent filtering with 40μm strainers Library was constructed under manufacture's instruction (Chromium Single Cell 3' Library & Single Cell 3ʹ v3 Gel Beads)