RNA-Seq of mus musculus: adult male femurs
Transcriptome sequencing was performed by Shanghai OE Biotech Co., Ltd (Shanghai, China). Total RNA was extracted using the RNeasy Lipid Tissue Mini Kit (QIAGEN, 74804) according to the manufacturers protocol. RNA purity and concentration were assessed using a NanoDrop 2000 spectrophotometer (Thermo Scientific), while RNA integrity was evaluated using a 2100 Bioanalyzer (Agilent). Libraries were constructed utilizing the VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina (NR604, Vazyme) as per the manufacturers instructions. Sequencing was performed on an Illumina Novaseq 6000 system (Illumina), generating 150 bp paired-end reads.