RNA-Seq of TAF15-NT OE cells_3
Total RNA was extracted with the EZ-press RNA Purification Kit and submitted to Novogene for sequencing. mRNA was enriched using oligo(dT) magnetic beads, fragmented, and reverse transcribed with random hexamer primers to synthesize first-strand cDNA; second-strand cDNA was synthesized using dUTP. After end repair, A-tailing, adapter ligation, fragment selection, USER digestion, amplification and purification, strand-specific libraries were generated and pooled according to effective concentration and desired output, then sequenced on an Illumina platform.