Nanopore seq of Nitrobacter vulgaris MLSD-S22
. Cell pellets were lysed and DNA was extracted using the Quick-DNA HMW MagBead Kit (Zymo Research, Irvine, California, USA) according to the manufacturers instructions. DNA was sequenced using a MinION Mk1c nanopore long-read sequencer (Oxford Nanopore, Oxford, United Kingdom) at the Institute for Systems Biology in Seattle, Washington, USA. Basecalling was performed using Dorado v 0.5.2 with the super high accuracy model dna_r10.4.1_e8.2_400bps_sup@v4.2.0 (Oxford Nanopore). A total of 477,072 reads were generated with a mean length of 3,898 bp.