GSM8726441: HeLa cells treated with RHPS4, biol rep 1; Homo sapiens; RNA-Seq
Total RNA was extracted with a paramagnetic bead technology, using the Negedia RNA extraction service (NEGEDIA srl), and quantified with the Qubit 4.0 fluorimetric Assay (Thermo Fisher Scientific). Libraries were prepared from 125 ng of total RNA using the NEGEDIA Digital mRNA-seq research grade sequencing service (Next Generation Diagnostic srl)1 which included library preparation, quality assessment and sequencing on a NovaSeq 6000 sequencing system using a single-end, 100 cycle strategy (Illumina Inc.).