Soil metagenome from Thailand: S_Z1_36r1
Metagenomic DNA was extracted from 0.25 g of soil using the DNeasy PowerSoil Kit. The V3-V4 hypervariable region of the 16S rRNA gene was amplified by PCR using the fusion primers 342F and 895R containing Illumina adapter and barcode sequences. PCR amplicons were purified and sequenced on the Illumina MiSeq platform (paired-end, 2x250 bp) to profile the soil bacterial community.