GSM9313106: Ccl25-flox Foxn1-Cre-Tg mouse thymic stromal cells; Mus musculus; RNA-Seq
Enzymatically digested thymic cells were mixed with Anti-CD45 MicroBeads to deplete hematopoietic cells. Single cell suspensions were generated and loaded onto the 10x Genomics Chromium platform following manufacturer recommendations in Chromium Next GEM Single Cell 3'v3.1 (Dual Index) User Guide. Cells were lysed and polyA transcripts from each partitioned cell were reverse transcribed and single cell barcoded. Library construction of single-cell barcoded cDNA libraries was performed on the 10x Genomics Chromium Connect instrument following manufacturer recommendations. In short, cDNA libraries were fragmented, had adapters ligated, and sample indices and additional Illumina sequencing adapters added on by limited cycle PCR. Libraries were quantified, normalized and loaded on a NovaSeq 6000 instrument using 10x Genomics recommended run parameters.