total RNA-Seq and dsRIP-Seq of Arabidopsis thaliana rnj null mutant complemented with RNJ versions modified for the GT1 domain
Sequencing libraries were constructed using the POPS platform (IPS2) from 300 ng of total RNA or 15-60 ng of immunoprecipitated RNA (see dsRIP section) using the Illumina TruSeq Stranded Total RNA with Ribo-Zero Plant kit (Illumina) with half reaction volumes. Library quality was checked using the Agilent High Sensitivity DNA Chip on the Agilent 2100 Bioanalyzer. Final quantification was performed using the Quant-iT PicoGreen dsDNA kit (Invitrogen) to generate an equimolar pool. Libraries were sequenced (2x752x150) on a NovaSeq X system (Illumina) by Biomarker Technologies GmbH, Mnster, to obtain approximately 30 and 10 million reads per total or IPed samples, respectively