PacBio HiFi long reads of Zea maize Ku123
High-molecular-weight (HMW) DNA was extracted from leaves using the Macherey-Nagel Nucleobond HMW kit according to the manufacturers instructions. Library preparation involved shearing and purifying the genomic DNA, followed by removal of single-strand overhangs, repair of DNA damage, end-repair, and A-tailing. Double-stranded positive and negative strands were ligated to form SMRTbell templates, which were subsequently size-selected.