GSM8606106: CS32 replicate 2; Homo sapiens; RNA-Seq
Four batches of 5 COs were pooled and flash frozen. Nuclei extraction was performed on frozen tissue as per manufacturer's instructions (Nuclei isolation kit, 10x Genomics). Freshly isolated nuclei were counted using a Countess® II FL Automated Cell Counter (ThermoFisher Scientific) and immediately processed using the Chromium Next GEM Single Cell 5' Reagent Kit v2 (10X genomics, Cat # 1000263). For each reaction, 16500 nuclei were loaded onto GEM Chip K for an expected recovery of 10,000 nuclei. For each reaction, 16500 nuclei were loaded onto GEM Chip K for an expected recovery of 10,000 nuclei. Gel Beads-in-emulsion were generated using the Chromium Controller followed by cDNA generation and amplification (13 cycles) as per manufacturer's instructions. For each sample, 50ng of cDNA was used for library generation.