eLW3_Heart_RNA (CRX873817)
Total RNA was extracted from frozen samples using TRIzol reagent (Invitrogen, USA), followed by mRNA collection using magnetic beads attached to poly-T oligos. The collected mRNA was then reverse-transcribed into cDNA. AMPure XP Beads were used for fragment enrichment and polymerase chain reaction (PCR) amplification to obtain strand-specific cDNA libraries