Swine Intestinal Epithelial Cells cocultured with heat-treated bactera-2
Total RNA was extracted using an appropriate RNA extraction kit. mRNA was enriched using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Strand-specific RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs), according to the manufacturer's instructions.