Nanopore RNA-seq of Ceratitis capitata mixed embryos at 6 hrs AEL
Medfly embryos were collected 6 hour after egg laying and snap frozen in liquid nitrogen to stop growth. The embryos were subsequently put in RNASafeguard reagent. Total RNA were extracted from a bulk of embryos, ERCC spiked in, and cDNA libraries generated which were sequenced with Nanopore technology.