GSM9825268: H9C2 cardiomyocytes, HG-Res group, biological replicate 3; Rattus norvegicus; RNA-Seq
Total RNA was isolated from H9C2 cells in each experimental group using Trizol reagent (Thermo Fisher Scientific, USA) according to the manufacturer's instructions. The purity and integrity of RNA were detected by a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, USA) and agarose gel electrophoresis, respectively. Only RNA samples with A260/A280 ratio between 1.8 and 2.0 and intact 28S and 18S rRNA bands were used for subsequent transcriptome sequencing. Transcriptome sequencing library construction and high‑throughput sequencing were performed by Novogene Co., Ltd. (China) using the Illumina NovaSeq 6000 platform. Briefly, mRNA was enriched from total RNA using oligo(dT) beads, followed by fragmentation into short fragments. First‑strand cDNA was synthesized using random hexamer primers and reverse transcriptase, followed by second‑strand cDNA synthesis. The cDNA fragments were end‑repaired, A‑tailed, and ligated with sequencing adapters. After PCR amplification and purification, the final libraries were quality‑controlled and sequenced on the Illumina NovaSeq 6000 platform to generate 150 bp paired‑end reads.