RNASeq of C acetobutylicum and C carboxidivorans consortium: Inoculum
The quantity and quality of RNA samples were assessed using the Qubit RNA HS Assay (Life Technologies) and the Fragment Analyzer system HS RNA Kit (15NT, Agilent). RNA-Seq libraries were prepared with the Illumina Stranded Total RNA Prep with Ribo-Zero Plus Microbiome (Illumina), following the manufacturers recommendations, starting with 0.2  0.5 g of total RNA as the input material, depending on availability. The final library was validated on a 2100 Bioanalyzer System (Agilent) using the Agilent DNA 7500 Assay Kit. The libraries were sequenced on the NovaSeq 6000 (Illumina) with a read length of 2x51 bp, following the manufacturers protocol for dual indexing. Image analysis, base calling, and quality scoring of the run were performed using the manufacturers software Real Time Analysis (RTA v3.4.4), followed by the generation of FASTQ sequence files.