Neutralizing antibody responses to common respiratory viruses are preserved following SARS-CoV-2 reinfection in a South African cohort
Viral nucleic acid was extracted from an oronasopharyngeal swab specimen using the MagMAX Viral/Pathogen Nucleic Acid Isolation Kit (Thermo Fisher Scientific, USA) according to the manufacturers instructions. Extracted DNA was quantified and prepared for whole genome sequencing using the Oxford Nanopore Technologies (ONT) Rapid Barcoding Kit SQK-RPB114.24. Briefly, adenoviral DNA underwent rapid barcoding and library preparation following the ONT protocol for multiplexed sequencing. Prepared libraries were pooled and loaded onto a R10.4.1 flow-cell and run on the MinION Mk1D sequencer. Raw sequencing data were basecalled and demultiplexed using ONT Guppy software, and downstream genome assembly and consensus sequence generation were performed using Genome Detective v2.24.1 bioinformatics workflows for adenovirus whole genome analysis.