WGS of Salmonella enterica subsp. enterica serovar Typhimurium strain 14028S (replicate 3)
Whole genome sequencing libraries were prepared from high-quality genomic DNA using the Twist Illumina-compatible library preparation kit following the manufacturers protocol. The workflow included genomic DNA fragmentation, end repair, adapter ligation, purification, and PCR enrichment to generate sequencing-ready libraries. Library quality and quantity were assessed prior to sequencing. The prepared libraries were subjected to paired-end sequencing (2  150 bp) on an Illumina platform to generate high-throughput whole genome sequencing data for downstream genome assembly, variant analysis, and comparative genomic analysis.