GSM9791341: S36_EXP2161RNA6_KO_aPD1_3; Mus musculus; RNA-Seq
Fresh tumor tissue was processed for total RNA extraction using the RNeasy Blood & Tissue Kit (Qiagen, Germantown, MD) per the manufacturer's instructions. RNA was quantified with the Qubit Fluorometer (ThermoFisher Scientific, Waltham, MA) and assessed for quality using the Agilent TapeStation 4200 (Agilent Technologies, Santa Clara, CA). Samples were prepared for RNA sequencing with the NuGEN Universal Mouse RNA-Seq Library Preparation Kit with NuQuant (Tecan Genomics, Redwood City, CA). Using 100 ng of RNA, cDNA and strand-specific libraries were generated following the manufacturer's protocol. Quality control included TapeStation size assessment and quantification via the Kapa Library Quantification Kit (Roche, Wilmington, MA).