Nanopore RNA-seq of Ceratitis capitata single embryos at 3 hrs AEL
Medfly embryos were collected 3 hour after egg laying and snap frozen in liquid nitrogen to stop growth. The embryos were subsequently put in RNASafeguard reagent. Total DNA and RNA were extracted from each embryo, ERCC spiked in, and cDNA libraries generated which were sequenced with Nanopore technology. DNA was used to sex the embryos. The file names contain unknown if sex was not determined, male for male embryo and female for female embryos.