RNA-Seq of Homo sapiens: empty vector transfection control in EV-D68-infected RD cells
RD cells infected with EV-D68 were transfected with empty vector as control. Total RNA was extracted from RD cells using TRIzol reagent according to the manufacturer's instructions. RNA integrity was assessed with an Agilent Bioanalyzer. Polyadenylated RNA [poly(A)+ RNA] was selectively enriched using oligo(dT) magnetic beads (library selection: other), then fragmented and reverse transcribed to cDNA. Libraries were prepared using the NEBNext Ultra RNA Library Prep Kit and sequenced on the Illumina HiSeq 4000 platform to generate 150 bp paired-end reads. Sequencing was performed by Shanghai Majorbio Bio-Pharm Technology Co., Ltd.