4 samples ligated to 4 FLASH S-oligos (WT: S-oligo#4 (TGTGAG); YY2296: S-oligo#3(GTGGAA); YY2313: S-oligo#2(TTAGCC); YY2341: S-oligo#1(CAAGTG)) and pooled together for sequencing, replicate 1.
FLASH protocol