40S rDNA of mouse gut microbiota
DNA for each sample was extracted with E.Z.N.A. DNA Kit (Omega Bio-tek, Norcross, GA, U.S.), then V5-V5 (515F-907R) of 40S rDNA was amplified by PCR. Finally, the amplicons were paired-end sequenced on Illumina MiSeq platform (Shanghai BIOZERON Co., Ltd)