RNAseq of ipsilateral mouse brain homogenates 1 day following collagenase-induced ICH vs naive controls
RNAseq of ipsilateral mouse brain homogenates 1 day following collagenase-induced ICH vs naive controls
Mice were PBS perfused and right (ipsilateral) hemispheres were extracted and homogenised using lysing matrix D tubes (MPBio) in a Qiagen Tissue Lyser II. Naive mice were perfused with PBS and right hemispheres were processed for RNA. RNA was isolated using the RNeasy lipid tissue mini kit (Qiagen) as per manufacturer's protocol. RNA-Seq libraries were generated using the TruSeq Stranded mRNA assay (Illumina, Inc.) according to the manufacturer's instructions.