GSM9743055: TCR sequencing sample from patient 51, infusion product; Homo sapiens; OTHER
RNA was extracted from cryopreserved PBMCs and TIL samples using the miRNeasy Mini Kit (Qiagen, Cat#217084) according to the manufacturer's protocol TCR repertoire libraries were constructed using the QIAseq Immune Repertoire TCR Panel (Qiagen, Cat#334651). Targeted library preparation included reverse transcription with TCR constant-region primers, unique molecular identifier (UMI)-integrated 5' adapter ligation, TCR-specific sequence enrichment, and dual indexing. Libraries were quantified using an Agilent 2100 Bioanalyzer and sequenced on an Illumina NovaSeq 6000 platform with 150-bp paired-end reads.