Impatiens thomsonii (CRX2110055)
Genomic DNA of new species was isolated from silica-gel-dried leaves using the standard CTAB method. DNA concentration was measured with the Qubit, while integrity was confirmed by 1% agarose gel electrophoresis. Subsequently, a DNA library was constructed using the VAHTS Universal Plus DNA Library Prep Kit for Illumina, and the library was sequenced on the Illumina NovaSeq 6000 sequencing platform with a PE150 sequencing strategy. The chloroplast genome and nrITS sequence was assembled using GetOrganelle v1.7.5.3. To assess the completeness of the final graph, Bandage v0.9.0 was used to visualize assembly graphs.