GSM9263467: CT5.3hTERT,Everolimus,24h_replicate1; Homo sapiens; RNA-Seq
RNA was extracted using the miRNeasy Micro Kit (Qiagen), and 27.5 ng of RNA per sample was treated with Heat-Labile Double-Strand-Specific DNase (Arcticzymes) to remove DNA contamination. Library construction was performed using the QuantSeq-Pool Sample-Barcoded 3′ mRNA-Seq Kit (Lexogen) according to the manufacturer's protocol.