GSM8726437: Matar Ageta Heat Stress, Pea, rep2; Pisum sativum; RNA-Seq
Total RNA from unopened flower buds at pre-anthesis stage from under CON and HS conditions were extracted using TRIzol Reagent (Ambion, Life Technologies) and purified using RNAeasy MiniElute cleanup kit (Qiagen, Germany) followed by on-column DNase-I treatment. Total RNA quantification and quality control was checked photometrically using NanoDrop OneC Microvolume UV–Vis Spectrophotometer (Thermo Fisher Scientific, USA) for 260/280 and 260/230 ratios and stored in -80 °C until RNA-Seq. RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.