mod_5 (CRX765086; CRA012252)
Microbial DNA was extracted from snap-frozen ileo-cecal valve contents of mice and DNA was quantified and quality controlled by agarose gel electrophoresis. The V3-V4 hypervariable regions of the bacteria 16S ribosomal RNA gene were amplified by PCR. Finally the amplicons were sequenced on an Illumina MiSeq platform.