GSM9170302: MB49 tumor cells; Mus musculus; RNA-Seq
MB49 cells in tissue culture were dissociated using 0.25% Trypsin/EDTA and passed through a 100-micron strainer to obtain a single cell suspension. Single cell RNA libraries from 10,000-20,000 cells per sample were generated using a Chromium Controller and the GEM-X Single Cell 5' kit from 10X Genomics. Library sequencing was performed by the Genome Technology Access Center at Washington University in St. Louis.