GSM9897232: iPSC-derived-microglia  co-maturation culture (T2); Homo sapiens; RNA-Seq
At DIV 28, cultures were dissociated using Accutase (20 min at 37°C) to obtain single-cell suspensions. Microglia monocultures received the same PLO/human laminin coating as co-cultures and were processed identically for a fair comparison, even though they required a shorter incubation than the denser neuron-glia networks. Single-cell suspensions were fixed for 24 hours at 4°C and stored with Enhancer at -80°C. Libraries were generated using the Chromium Next GEM Single Cell Fixed RNA Sample Preparation Kit (10x Genomics, PN-1000414) and the Chromium Single Cell Flex Kit (10x Genomics, protocol CG000527 Rev F). Samples were incubated for 20 hours at 42°C with an anti-human probe set. Each condition received a unique probe barcode (BC001-BC004) for multiplexing. After pooling and washing, three lanes of a 10x Chip Q were loaded, with each condition represented in every final lane/library (FLEXLib1-3).