RNA-Seq of klebsiella pneumoniae: JNQH373
The bacterial strains were grown to mid-log phase at 37C in LB broth. Cells were harvested and treated with RNAprotect (Qiagen), followed by extraction using UltraClean RNA isolation kits (MoBio). cDNA libraries were constructed with ScriptSeq Complete Gold kits (Epicentre Biosciences) and were sequenced on an Illumina HiSeq instrument.