Y2H-seq of empty vector interaction screen  selective media pool Fw
This sample represents the empty-vector control for the Y2H-seq screen. The Gal4 DNA-binding domain vector without the Mj-ISE6 insert was transformed into Saccharomyces cerevisiae PJ69-4A and super-transformed with the same high-complexity Solanum lycopersicum cDNA library used for the bait screen. Colonies growing on selective medium (SD-LWH + 3-AT) were pooled to identify prey sequences capable of background growth in the absence of the bait protein. Plasmid DNA from the pooled colonies was extracted, prey inserts were PCR-amplified, and sequencing was performed on an Illumina HiSeq 2000 platform using paired-end 125-bp reads. These data serve as the negative control for filtering non-specific interactors in the Mj-ISE6 Y2H-seq analysis.