Whole genome sequencing of single TDP43 negative cell PTA amplified DNA from homo sapien prefrontal cortex
Single neuronal nuclei were isolated using FANS with co-staining of NeuN and TDP-43 antibodies from frozen brain (prefrontal cortex). The nuclie were lysed and the genome amplifeid using primary template-directed amplification (PTA). Libraries were prepared using Kapa HyperPlus modified for PTA and sequenced to a target depth of 45X on an Illumina NovaSeq 6000.