metabarcoding of seawater eDNA: MiFish-U targeting 12S for Actinopterygii
eDNA extracted with QIAGEN Dneasy Blood and Tissue kit, PCR performed with MiFish-U primers, cleaned with Dneasy PowerClea Pro Cleanup Kit (QIAGEN), size-selected using the GeneRead Size Selection Kit (Qiagen), 2nd PCR library preparation with Illumina TruSeq indices, sequencing on MiSeq with v3 600 kit