GSM9380161: Pig Frontal Cortex, Animal ID 43-14, WT, female; Sus scrofa; RNA-Seq
Tissues were flash frozen on liquid nitrogen and stored at -80*C until use in 10X Nuclei Isolation Kit (PN-1000493). Approximately 25 mg of tissue was used and the  kit protocol and provided reagents were used to isolate and suspend nuclei. Libraries were constructed according to the 10x Genomics Single Cell Gene Expression 3' v3 library preparation protocol, and followed by sequencong on the Illumina NovaSeq 6000.