GSM9078365: Rep2 GEX Library; Mus musculus; RNA-Seq
Immunocytes were released by mechanically disrupted and filtered through 40 μm cell strainer to get single-cell suspensions. CD4+ T cells were enriched using Dynabeads Untouched Mouse CD4 Cells Kit (Invitrogen). Cells were then sorted with a FACSAria III, and sent to the Broad Institute Genomics Platform for encapsulation and 10x Genomics protocols. Libraries were prepared according to the 10x Genomics 5'v2 protocol for GEX and FBC