GSM8465399: Bone marrow derived macrophages(BMDMs),Tshrf/f, TSH,1ng/mL,24 hours,rep2; Mus musculus; RNA-Seq
Total RNA was isolated from each thymic sample using the RNAmini kit ( Qiagen,Germany). 2ug of total RNA was used for the construction of sequencing libraries. Strand-specific libraries were constructed using the TruSeq RNA sample preparation kit (Illumina, San Diego, CA, USA) Sequencing was carried out using the Illumina Novaseq 6000 instrument by the commercial service of Genergy Biotechnology Co. Ltd. (Shanghai, China).