GSM8831050: Lung cell, aged, uninfected, BCR; Mus musculus; RNA-Seq
Cells obtained from the left lung were processed by a Dead cell removal kit (Miltenyi) and positive selection using CD45+ microbeads (Miltenyi). The harvested cells were filtered and were processed using Chromium Controller (10x Genomics), following the manufacturer's protocols. Single-cell suspensions were processed using the Chromium Next GEM Single Cell 5′ Reagent Kits v2 (10x Genomics). Gene expression libraries were prepared according to the manufacturer's protocol. cDNA libraries were sequenced on the NextSeq500 platform (Illumina) to reach a minimum depth of 50,000 reads/cell.  Enrichment of V(D)J segments and construction of the single-cell TCR library were performed using the Chromium Single Cell Mouse TCR Amplification Kit (10x Genomics). Similarly, the single-cell BCR library was constructed using the Chromium Single Cell Mouse BCR Amplification Kit (10x Genomics).