Role of LAIR-1 in the regulation of macrophages in fibrotic lungs
Fibrotic lungs were collected at day 14 following intranasal administration of bleomycin. Lung macrophages were sorted as CD11b+CD64+ cells  on BD FACSMelody Cell Sorter. Cells were directly lysed with RA-1 lysis buffer for RNA extraction (NucleoSpin RNA kit, Macherey Nagel).