GSM9435901: DFNC3; Mus musculus; RNA-Seq
Total RNA was isolated using a TRIzol total RNA extraction kit (TIANGEN,Cat.No.DP424), which yielded > 2 μg of total RNA per sample. Illumina library construction was performed according to the manufacturer's instructions (Illumina,USA). Oligo-dT primers are used to transverse mRNA to obtain cDNA(APExBIO, Cat. No. K1159). Amplify cDNA for the synthesis of the second chain of cDNA. Purify cDNA products by AMPure XP system (Beckman Coulter, Beverly, USA)