Environmental DNA metabarcoding of Macao wetland (18S)
Environmental DNA extracted from surface water samples collected from urban wetlands in Macao was amplified targeting a ~400 bp fragment of the 18S rRNA V4 region using primers AReuk454FWD1 and TAR-eukREV3. PCR products were purified, pooled, and prepared into sequencing libraries following BGIs Meta rDNA Amplicon protocol. Library quality and insert size were confirmed using an Agilent 2100 Bioanalyzer, and sequencing was conducted on the DNBSEQ-G400 platform using paired-end 300 bp reads.