GSM9685914: Liver, floxed control_Fed_rep2; Mus musculus; RNA-Seq
Total RNA was isolated from frozen liver tissues using ReliaPrep™ RNA tissue miniprep system (Promega). Quality and quantity of total RNA were evaluated using a Bioanalyzer (Agilent Technologies, Santa Clara, CA). Strand-specific RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs, Ipswich, MA) with the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England BioLabs), according to the manufacturer's instructions.