GSM8795663: Spinal cord, 50 days, wild type, rep8; Mus musculus; RNA-Seq
Spinal cord  tissues were homogenized with QIAzol Lysis Reagent (Qiagen) using the TissueLyser LT (Qiagen). RNA was isolated with Direct-zol RNA Miniprep (Zymo Research) according to the manufacturer's instructions. Ribosomal RNA depletion, RNase R digestion , double-stranded cDNA synthesis, adapter ligation (Illumina), second-strand cDNA elimination, first-strand cDNA purification for PCR.