GSM9708658: iN, CTL, time 0, donor A48, batch J082_4_Batch_1; Homo sapiens; RNA-Seq
Bulk RNA-seq libraries were generated from total RNA isolated from samples collected at 0h and 336h (14 days) post IR. Mock IR non-transduced fibroblasts were collected at 0h post IR while mock IR iNs were collected at 336h to match differentiation timelines in the CTL iNs, which is not present in the non-transduced fibroblasts. The cells were lysed in the lysis buffer plus 2-Mercaptoethanol (Sigma, M3148-25ML) according to manufacturer's instructions (VWR, BIO-52073). RNA was isolated according to the protocol and sent out to Novogene for sample QC, library preparation, and sequencing. Sequencing was performed at 20M paired-end reads per sample on the NovaSeq X Plus Series (PE150) (Illumina).