GSM9170550: Control_female; Mus musculus; RNA-Seq
Nuclei were isolated from the muscularis propria of the colon using the 10X Chromium Nuclei Isolation Kit as of the manufacturer's instructions. Briefly, colons were collected and pinned in a silicone-lined petri dish to remove the mucosa and submucosa. The muscularis propria was then minced into 2-5mm fragments before conducting the nuclei isolation procedure. snRNA-seq - Chromium Single Cell 3' Reagent Kit (v3.1)