GSM8488487: METTL3 iKO hEPSC in P0, rep1; Homo sapiens; ATAC-seq
50,000 cells were collected and washed twice in pre-cold PBS without DNase. Nuclei were then isolated, lysed and incubated with Tn5 transposase according to manufacturer's protocol (Vazyme, TD501). Libraries were prepared by amplifying DNA for 12 cycles using TruePrep DNA Library Prep Kit (Vazyme).