RNA-seq of Arabidopsis thaliana seeds from WT and DREB2G overexpression (OE) lines
RNA-seq of Arabidopsis thaliana seeds from WT and DREB2G overexpression (OE) lines
Total RNA were extracted from pooled Arabidopsis thaliana seeds 10-12 days after flowering (DAF). Arabidopsis thaliana seedlings are grown chambers under constant temperature of 23°C and a photoperiod of 16 h light (100‐150 μmol m−2 sec−1 354 illumination)/8 h dark. Total RNA extraction was carried out using the Monarch Total RNA Miniprep Kit (New England Biolabs), following the manufacturer's protocols. Subsequently, DNase I (New England Biolabs) was employed to remove genomic DNA, according to the manufacturer's instructions. RNA-seq libraries were generated using NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) for Illumina according to the manufacturer's instruction