GSM8898338: Infected; Bos taurus; RNA-Seq
The cells and viruses were then incubated together for 24 hrs at 37oC supplemented with 5% CO2. After 24 hrs incubation, cells were centrifuged at 400xg for 5 mins at 4oC to remove debris and ambient host and viral DNA/RNA. The cells were again subjected to Dead Cell Removal Kit (Miltenyi Biotec) for removing dead cells and debris and to reach the viable cell target of more than 70% live cells for scRNA-seq experiments. The library was constructed using Chromium Next GEM Single Cell 5' Reagent Kits v2 (10X genomics) following manufacturer's instructions. Briefly, single-cells suspensions were loaded onto a Chromium Single Cell Chip (10x Genomics) for co-encapsulation with barcoded Gel Beads at a target capture rate of ~10000 individual cells per sample using Chromium Controller (10x Genomics).