Environmental DNA metabarcoding of Macao wetland (12S)
Environmental DNA was extracted from surface water samples collected from urban wetlands in Macao. DNA extracts were used as templates for PCR amplification targeting a ~170 bp fragment of the mitochondrial 12S rRNA gene using MiFish-U primers. Amplicons were purified and pooled for library preparation following BGIs standard amplicon sequencing workflow. Libraries were quantified with an Agilent 2100 Bioanalyzer, then sequenced on the DNBSEQ-G400 platform using paired-end 300 bp reads.