GSM9652000: human_CSF_PPMS_2; Homo sapiens; RNA-Seq
Frozen CSF was thawed at 37°C in complete growth medium (10% FBS in RPMI), washed, and then processed as described in 23, with the modification that cells were not sorted due to high cell loss observed during this step; fresh CSF was processed directly. To generate single-cell libraries, cell suspensions were loaded onto the Chromium Single Cell Controller with the Chromium Single Cell 5′ Library & Gel Bead Kit v.1.1/v2 (both from 10X Genomics) in adherence to the manufacturer's guidelines. Chromium Single Cell 5′ Library & Gel Bead Kit v.1.1/v2 (both from 10X Genomics