scRNA-seq of mus musculus: adult male testis
Testicular scRNA-seq was performed on PND70 mice from the TA 1 mg/kg and Control groups. Briefly, testicular tissues were enzymatically dissociated into single-cell suspensions. Cell concentration was determined using a Countess 3 Automated Cell Counter (Thermo Fisher). Cell suspensions were mixed with Trypan Blue, and viability was assessed by inverted microscopy. Only samples exhibiting > 80% viability were loaded onto Single Cell A Chips (10 Genomics, Chromium) to generate single-cell Gel Bead-In-Emulsion (GEMs). Libraries were prepared using Single Cell 3' Reagent Kits v3.1 and sequenced on the Illumina platform by Genedenovo Biotechnology Co., Ltd (Guangzhou, China).