Mus musculus scRNA
Briefly, viable cells (>80% viability) were resuspended at 2052000 cells/L and encapsulated into GelBeadsinEmulsion (GEMs) for cell lysis and cDNA reverse transcription. After GEM disruption, cDNA was purified, amplified, and used to construct sequencing libraries. Libraries were quantified and sequenced on the DNBSEQ platform in pairedend 100bp mode, achieving a median depth of 30,000 reads per cell.