WGS of Podarcis muralis: Common Wall Lizard
Genomic DNA was extracted from tissue samples using a standard phenol chloroform extraction protocol (Sambrook et al. 1989). To generate whole genome sequences from individual samples, we prepared individual libraries using Illumnia TruSeq Whole Genome library preparation kits and libraries were sequenced (PE 2x150) on an Illumina NovaSeq 6000 sequencer at Novagene or the University of Illinois Roy J. Carver Biotechnology Center. A total of 35 samples were sequenced and each sequencing run generated at least 5M 150bp paired end reads per sample.