Metatranscipts_P13_2020_1020cm
We extracted RNA from six subsets of 30 mg of soil from the 10-20 cm depth using the Powermicrobiome RNeasy kit (QIAGEN), purified the samples using the OneStep PCR Inhibitor Removal Kit (ZYMO), digested the DNA using TURBO DNA-free Kit (Invitrogen), and pooled the samples with RNA Clean & Concentrator-5 (ZYMO).To remove ribosomal rRNA, we used QIAseq FastSelect -rRNA 5S/16S/23S kit and -rRNA Plant Kit (QIAGEN). Processed RNA was converted to cDNA using NEBNext Ultra II Directional RNA Library Prep Kit (NEB, USA).