GSM9081355: A549 cells, circulating tumor cells, replicate 3; Homo sapiens; RNA-Seq
RNA extraction was performed using Macherey-Nagel RNA NucleoSpin kit. Library preparation was performed using the Illumina Stranded mRNA Prep Ligation kit (Illumina) following the manufacturer's protocol. All sequencing libraries were constructed from 100 ng of total RNA according to the manufacturer's instruction.