RNA-seq of Mus_musculus:L7D2_3_1.fq.gz
Singleron bulk-RNA is a well-based platform designed for bulk transcriptome analysis across multiple samples simultaneously. It utilizes well barcodes to differentiate samples and combines UMI technology to accurately assess the relative abundance of transcripts. The core methodology involves capturing mRNA released from cell lysis using magnetic beads, followed by reverse transcription (RT) and PCR amplification. All amplified products are then mixed, purified to obtain a mixed cDNA containing samples from multiple sources. Subsequently, the cDNA is fragmented, end-repaired, adapter-ligated, and amplified to construct the transcriptome library. The final library can be sequenced on Beijing Genomics Institute(BGI)DNBseq-T7 sequencing platform using the PE150 strategy.