16S rRNA sequencing of Eriosma harunire: foundress adult, Hokkaido Univ, Sapporo
Genomic DNA from aphid samples was extracted using the QIAGEN DNeasy Kit. 16S rDNA amplicons (V1-V2, V3-V4) were amplified using Zymo Research Quick-16STM primers with 30 PCR cycles. DNA quality was assessed with TapeStation HS D5000. Libraries were prepared with TruSeq Nano DNA Prep and sequenced on the Illumina MiSeq platform at the Trans-Omics Facility, Japan.