GSM9578175: K52Q-V5 trehalose biol rep 2; Vibrio cholerae C6706; RNA-Seq
Direct-zol RNA Miniprep Plus Kit following manufacturer's instructions Library preparation was performed using Illumina's Stranded Total RNA Prep Ligation with Ribo-Zero Plus kit and 10 bp unique dual indices (UDI). The kit employs dUTP incorporation during second-strand cDNA synthesis to confer strand specificity, producing RF-oriented paired-end reads. Sequencing was performed on an Illumina NovaSeq X Plus generating paired-end 150 bp reads. Demultiplexing, quality control, and adapter trimming were performed with bcl-convert v4.2.4