Female 2
As directed by the manufacturer, total RNA was extracted from theantenna using RNAprep Pure Micro Kit.An Agilent 2100 Bioanalyzerand a NanoDrop ND-2000 spectrophotometer were used to confirm thequality of the total RNA.A SMARTer  Ultra  Low RNA kit was usedto generate a SMARTer library for further sequencing. At the NovogeneBioinformatics Institute,RNA sequencing libraries were constructed andusing a paired-end(PE150 bp)methodsequencedon the lluminaNovaSeq X Plus.