GSM8728046: Karpas422-EZH2-GOF-rescue-1; Homo sapiens; RNA-Seq
Total RNA was isolated from cultured cells using RNeasy Mini Kit (Qiagen) with on-column DNA digestion. RNA samples were analyzed on the Bioanalyzer RNA 6000 Pico (Agilent) prior to library preparation. RNA libraries were converted to cDNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) and NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB) according to the manufacturer's instructions. All libraries were analyzed by TapeStation prior to sequencing. Single-end sequencing was performed on the Illumina NextSeq 500 sequencer.