RNA-seq of Arabidopsis: inflorescence
RNC-mRNA was extracted with an RNA Extraction Kit. The rRNA in total RNA was wiped out with Ribo-Zero rRNA Removal solution (Illumina, RZH1046) and the left RNA was purified with Agencourt RNA Clean XP Beads (Beckman, A63987) and used as template to synthesis the first strand cDNA with ProtoScript  Reverse Transcriptase (NEB, M0368). Agencourt AMPure XP Beads (Beckman, A63880) were used to purify the obtained double strand cDNA synthesized by NEBNext Second Strand Synthesis Enzyme Mix (NEB, E7550). The cDNA tail was prepared with NEBNext End Prep Enzyme Mix (NEB, E7442) and the NEBNext Adaptor for Illumina (NEB, E7335) was added to the cDNA. Then the DNA was screened by AMPure XP beads to obtain fragments with 250-350bp in size. After amplification with NEBNext Q5 Hot Start HiFi PCR Master Mix (NEB, M0543), the sample was purified with AMPure XP beads (1.0x) to obtain the final library.