BMOL bulk RNA-seq, siCon, rep3
Total RNA was extracted using TRIzol reagent (Invitrogen, USA) following the manufacturers instructions. RNA integrity was assessed with the Agilent 2100 Bioanalyzer. Sequencing libraries were constructed using the Illumina TruSeq RNA Sample Preparation Kit according to the manufacturers protocol. Paired-end sequencing (150 bp) was performed on the Illumina HiSeq 2500 platform.