GSM9764712: 665a9191e8f6af5c3868adcf--L1LIE2700014_TT_seq_shpaics_3.raw.fastq.gz; Mus musculus; RNA-Seq
Mouse primary tumor cells were labeled with 400 μM 4-thiouridine for 10 min at 37 °C and were then lysed with TRIzol. RNA extraction, spike-in and biotinylation were performed as shown before. Biotinylated RNA was isolated using Dynabeads MyOne Streptavidin C1. The 4sU-labeled RNA was subsequently eluted with freshly prepared 100 mM DTT and further purified using Sera-Mag carboxylate-modified magnetic beads. TLibrary preparation was carried out with the NEBNext Ultra II Directional RNA Library Prep Kit.