GSM9472087: Syn D14 liver EC; Mus musculus; RNA-Seq
Endothelial cells were collected 4 days or 14 days after transplant and isolated from the liver via flow staining and sorting for Cd45- Cd11b+ Cd31+. Up to 10,000 cells per condition were targeted for scRNA-seq via the 10x Genomics Flex singleplex kit (BC001) following the manufacturer's protocol. Libraries were sequenced on an Illumina NovaSeq6000.