RNA-Seq of homo sapients PDAC:lowTIN-2
RNA was extracted from these cell samples with a Qiagen miRNeasy Mini Kit (Cat # 217004). Additionally, DNase digest was performed with a Qiagen RNase-Free DNase Set (Cat # 79254) to remove unwanted DNA. RNA quantity and quality were assessed by Qubit RNA HS Assay Kit (Cat # Q32855) and Agilent RNA 6000 Nano Kit (Cat #5067-1511) respectively and prepared for sequencing with the Lexogen SENSE mRNA-Seq Library Prep Kit V2 (Cat # 001.96). After library preparation was completed, the quality of the libraries was checked on an Agilent 2200 TapeStation using an Agilent High Sensitivity D5000 ScreenTape (Cat # 5067-5592) and library concentration was determined by Qubit dsDNA HS Assay Kit (Cat # Q32854) before proceeding to sequencing. While human PDAC samples with different tumor innervation status were sequenced (paired-end, 150 bp) on an Illumina NovaSeq 6000 using a single S2 flowcell, the in vitro cell models were sequenced (paired-end, 100 bp) on Illumina HiSeq2000.