HTGTS-Rep-Seq for Humanized Antibodies that Broadly Neutralize Omicron Sub-variants
HTGTS-Rep-Seq for Humanized Antibodies that Broadly Neutralize Omicron Sub-variants
Splenic B cells used for HTGTS-rep-seq were purified from unimmunized 5-8 weeks old mice by MACS® Microbeads according to the manufacturer's protocol. In brief, spleens were dissected out from unimmunized mice, prepared into single cell suspensions and stained with anti-B220 Microbeads for 20 minutes at 4°C. The splenic B cells were collected using the LS column and MACSTM Separater.  Genomic DNA was extracted from purified splenic B cells using the DNeasy Blood & Tissue Kit (Qiagen) following the manufacturer's protocol. The libraries of HTGTS-Rep-seq was constructed as previous described (Lin et al., PNAS 2016) 