GSM8581978: plate6_day6; Homo sapiens; OTHER
AMPure XP Embryos from embryonic days 5-6 were collected in Corujo-Simon et al. in which scRNA-seq was performed using the RNA half of the scNMT-seq method. In this study we used the gDNA from the same cells to generate DNA methylation and chromatin accessibility data using the published scNMT-seq protocol (52). Briefly, genomic DNA was purified using AMPure XP beads then bisulfite converted with the Zymo EZ-96 DNA Methylation-Direct MagPrep kit. Sequencing libraries were then prepared from bisulfite converted fragments via random primed synthesis using oligos containing Illumina primer sequences, followed by indexing PCR.