GSM8831561: 147 pooled monoclonal gastruloids derived from mouse embryonic stem cells (plate 4), sci-RNA-seq3; Mus musculus; RNA-Seq
For the cell extraction contructing the sci_RNA_seq3 library, we used cell fixation protocol published by Martin et al., Nature Protocol 2023 (doi: 10.1038/s41596-022-00752-0, PMCID: PMC9839601). For the cell extraction contructing the eight_gastruloids and tree_of_trees library, we used 10X Genomics' scRNA-seq kit (Chromium Next GEM Single Cell 3' HT v3.1). For the sci_RNA_seq3 library construction, we used single-cell combinatorial indexing protocol (sci-RNA-seq3) by Martin et al., Nature Protocol 2023 (doi: 10.1038/s41596-022-00752-0, PMCID: PMC9839601). For the eight_gastruloids and tree_of_trees library, we used 10X Genomics' scRNA-seq kit (Chromium Next GEM Single Cell 3' HT v3.1).