WGS of Enterobacter cloacae species complex: Lower Respiratory Tract
Total genomic DNA was prepared using the KingFisher Cell and Tissue DNA kit (ThermoFisher Scientific, Waltham, MA, USA) or the MagMax DNA Multi-Sample Ultra 2.0 extraction kit (ThermoFisher) on a KingFisher Flex Magnetic Particle Processor (ThermoFisher). DNA libraries were constructed using either the Nextera XT library construction protocol and index kit or the Illumina DNA prep (Illumina, San Diego, CA, USA) with sequencing performed on either a MiSeq Sequencer with a MiSeq Reagent Kit v3 (600 cycles) or a NextSeq 1000 Sequencer using NextSeq1000/2000 P2 Reagents (300 cycles).