GSM8797561: B16-IFNA4 Tumours (Day 8) mouse 1 biol rep 3; Mus musculus; RNA-Seq
Tumours were harvested and diced using a scalpel before being resuspended in RPMI supplemented with 3 mg/mL collagenase III (Worthington Biochemicals, USA) and 5 μg/mL DNAse I (Sigma-Aldrich, USA) for 30 mins at 37o C. Samples were neutralised in RPMI-10% FCS. Resulting single cell suspensions were passed through a 100 μM nlyon filter and centrifuged before being finally resuspended in FACS buffer (1x PBS, 1% Bovine Serum Albumin (BSA, Bovagen, USA), 5 mM EDTA). Single cell suspensions from digested tumours were processed with V3 chemistry using the Chromium Next GEM Single Cell 3' Gel Bead Kit v3.1 (16 rxns, PN-1000122, 10X Genomics) Chip G and Chromium Next GEM Single Cell Library Prep Kit (16 rxns, PN-1000190, 10X Genomics), according to manufacturer's protocol.