GSM9001405: Human Tenon's fibroblasts (HTFs), day0; Homo sapiens; RNA-Seq
We used Trizol LS Reagent (Thermo Fisher Scientific, Waltham, MA, USA) to extract Total RNA, following the manufacturer's instructions. To eliminate contaminating DNA, the RNA was treated with DNase I (Promega). Total RNA samples from iRPCs and parental HTFs were submitted to the Genomics commercial sequencing facility for Bioanalyzer quality control analysis and Illumina Next Generation Sequencing. All submitted samples had an RNA integrity number (RIN) > 8. Stranded TruSeq cDNA libraries with poly dT enrichment were prepared from total RNA from each sample according to the manufacture's protocol.