GSM8833284: Donor4, Th2 cells, Veh treated, Not Activated; Homo sapiens; RNA-Seq
Total RNA was extracted using RNEasy micro kit (Qiagen) using manufacturer instructions. RNA quality was determined using RNA ScreenTape Analysis (Agilent) and manufacturer instructions. All samples had a RIN score > 9.8. 400ng of RNA was used for subsequent analysis. Illumina Stranded mRNA Prep, Ligation protocol was used according to manufacturer instructions.