WGS of Eutagenia sp.: adult whole organism
Individual was killed and preserved in 100% ethanol and stored at -20C. DNA extraction was performed following a commercial bead-based protocol (Biosprint 96 DNA Blood kit, Qiagen) as implemented in the automated KingFisher Flex system (Thermo Fisher scientific). Extracted gDNA concentration and quality were assessed using TapeStation (Agilent). Library construction using the Illumina TruSeq DNA Nano prep kit (350 bp insert) and sequencing on the Illumina NovaSeq6000 platform (2  150 bp paired-end reads), were performed at Macrogen Europe (Maastricht, Netherlands). Target coverage was approximately 20X (6 Gb of paired-reads, assuming a genome size of 0.3 Gb).