Amplicon-seq of DSX5
Total genomic DNA was extracted from fins tissues of the collected A. dabryanus using the TIANamp Genomic DNA Kit (Tiangen Biotech, Beijing, China) following manufacturer guidelines. Briefly, approximately 20 mg of fins tissue was homogenized, lysed using proteinase K, and subjected to spin-column-based DNA purification. eDNA was extracted using the Jianshi DNA Extraction Kit for Water Samples (Jianshi Biotech, Wuhan, China).