GSM8465541: pool2_gene_expression; Homo sapiens; RNA-Seq
CD4 T cells were flow sorted from cryopreserved peripheral blood mononuclear cells and labelled with TotalSeqC oligo-tagged antibodies (available from BioLegend) against two ubiquitously expressed cell surface proteins (CD298 and β2 microglobulin). Six individually barcoded samples were pooled (one pool for first infection and a separate pool for third infection) and the concentration of each pool was carefully measured and adjusted to 1262 cells per microlitre. Each pool was then loaded into 2 wells of an A Chip, which itself was loaded onto a 10X Genomics Chromium Controller. Note that we intentionally super-loaded the Controller in order to capture approx. 30,000 singlets for each infection across 2 wells.