GSM9542836: CTX mouse granulosa cells, rep2; Mus musculus; RNA-Seq
Total RNA was extracted from granulosa cells, and RNA integrity and quantity were accurately detected using an Agilent 2100 Bioanalyzer (Agilent Technologies, CA, USA) to ensure qualified samples for subsequent experiments. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was prepared after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was quantified using Qubit and real-time PCR, and fragment size distribution was detected by a bioanalyzer.