The small cell lung cancer cell line NCI - H446, after treated with cisplatin and etoposide, and withdrawal 30 day, was subjected to RNA - Seq.
The raw data was filtered with SOAPnuke (v1.5.2) [1] by 1) Removing reads containing adapters (adapter contamination); 2) Removing reads whose unknown base ('N' base) ratio is more than 10%; 3) Removing reads whose low-quality base ratio (Base quality less than or equal to 15) is more than 50%, afterwards clean reads were obtained and stored in FASTQ format.