GSM8796715: Cultured cortical neurons, T6B, replicate 3 (TNRC6 RIP); Mus musculus; RIP-Seq
Cells were washed once with ice-cold DPBS and lysed with whole cell lysis buffer prior to IP. Total RNA (IP and input) was extracted with RNeasy® Mini Kit Total RNA samples were prepared into RNAseq libraries using the NEBNext UltraExpress™ RNA Library Prep Kit for Illumina. Briefly, for each sample 200 ng total RNA was polyA selected, fragmented and converted to double stranded cDNA followed by ligation of sequencing adapters. The libraries were then PCR amplified 12 cycles using barcoded PCR primers, purified and size selected using AmpureXP beads from Beckman Coulter.