High-Depth RNA-Seq of NmB MC58 modA11 ON: sample 3
Total RNA was extracted (TRIzol) from Neisseria meningitidis grown aerobically to mid-log phase in GC broth supplemented with 30 M desferrioxamine (iron-deplete conditions) at 37 C with 200 rpm shaking. Stranded RNA-seq libraries were prepared using the Illumina Ribo-Zero Gold stranded protocol (rRNA depletion; dUTP-based strand preservation) and sequenced on an Illumina NovaSeq X Plus (TruSeq SBS v3) to generate 2151 bp paired-end reads. Demultiplexing produced gzipped FASTQs using Illumina DRAGEN BCL Convert (94% bases Q30 for all samples).