GSM8970878: PNPCa mouse fraction, p0, Experiment 48_22,  #1; Mus musculus; OTHER
RNA was extracted from PDX tissue-derived stromal (mouse) fraction cells and PDX-fibroblasts lysed in TriPure Isolation Reagent (Sigma-Aldrich). RNA extraction was performed using the Direct-zol RNA Microprep kit (Zymo research). RNA concentration was measured with NanoDrop™ 2000/2000c Spectrophotometer and with QubitTM RNA Broad Range (BR) Assay Kit (ThermoFisher). RNA quality was assessed through the Agilent Total RNA 6000 Nano Kit (Bioanalyzer) or with the Qubit™ RNA IQ Assay Kits (Thermo Fisher) RNA samples with an amount > 200ng and RNA integrity number >6 were sent for sequencing. Samples were sent to Alithea Genomics (Lausanne, Switzerland) for sequencing using the BRB-seq method