GSM9761448: mTeSR1; Homo sapiens; OTHER
Genomic DNA extraction were performed using the Qiagen Genomic DNA Extraction Kit.  A total of 100 ng for each sample was used as input for the NEBNext Enzymatic Methyl-seq kit Library PCR amplification was done for 5 cycles using NEBNext Multiplex Oligos for Enzymatic Methyl-seq. The final libraries were quantified with the Qubit dsDNA HS Assay kit. Quality of the libraries was assessed on Agilent's 4200 TapeStation, using the High Sensitivity D1000 kit. Quantification by qPCR was performed using the KAPA Illumina Library Quantification Kit. Equimolar amounts of each library were pooled and quantified by qPCR before PE-150 sequencing on a 10B 300 cycle shared flow cell on the NovaSeq X Plus.