GSM6705262: Kasumi-1, CBFB, KD, 17151; Homo sapiens; OTHER
cells were bound to magnatic beads and incubated with overnight with the corresponding antibodies, then treatedwith  pAG/MNase for 1 hour at 4 C. DNA fragments were releasted at 37 C and extracted with Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v). CUT&RUN libraries were prepared using starting from 10-30 ng input and constructed with the KAPA HF polymerase and barcoded with Illumina TruSeq Barcodes.  10-30 ng of DNA was used as starting material  Libraries were amplified using 14 cycles on the thermocycler. Post amplification libraries were purfied with  Agencourt AMPure XP beads from Beckman Coulter. Libraries were validated using the Agilent High Sensitivity DNA Kit.