Illumina NovaSeq 6000 paired end sequencing
Reads processed via nf-core/eager v2.4.1. Aligned to human reference g1k v37 decoy using BWA aln (-n 0.01, -k 2, -l 1024). Analysis restricted to merged reads. BAM files filtered using Samtools (v1.12) with a mapping quality threshold (MQ) � 25 and minimum read length � 30bp. PCR duplicates removed using DeDup (v0.12.8).
Double-stranded libraries prepared following Meyer & Kircher (2010) with partial UDG treatment according to Rohland et al. (2015). Target enrichment performed via in-solution hybridization using the myBaits Expert Human Affinities Kit �Prime Plus�.