GSM9177112: AAA 172; Mus musculus; RNA-Seq
Total RNA was isolated from the aortic tissue specimen using the RNeasy® Plus Universal Kit Mini 50 (Qiagen GmbH, Hilden, Germany). The library preparation was performed according to the manufacturer's protocol using the 'VAHTS™ Stranded mRNA-Seq Library Prep Kit V6' for Illumina®. Briefly, 500 ng total RNA were used as input for mRNA capturing, fragmentation, the synthesis of cDNA, adapter ligation and library amplification. Bead purified libraries were normalized and finally sequenced on the HiSeq 3000/4000 system (Illumina Inc. San Diego, CA, USA) with a read setup of SR 1x150 bp.