GSM9110886: C-DRG; Mus musculus; RNA-Seq
Freshly dissected C3-C5 DRG was first homogenized in cold calcium and magnesium-free Hanks' balanced salt solution. DRGs were digested with Collagenase A (0.2mg/ml) and Dispase ®II (1.5mg/ml) in the oxygenated DMEM for 120 min at 37 °C. After filtering through a cell strainer (70 μm), the cell homogenate was mixed with 10% Percoll (Cytiva, #17089101) for myelin removal and enrichment. The cellular suspensions were further processed with Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 (PN- 120237) or v3 (PN-1000094) according to the manufacturer's instructions to construct the scRNA-seq library.