Group_LEP_D2 (CRX1868641; CRA028174)
Total genomic DNA was extracted using DNA Extraction Kit following the manufacturer's instructions. Concentration of DNA was verified with NanoDrop and agarose gel. The genome DNA was used as template for PCR amplification with the barcoded primers and Tks Gflex DNA Polymerase (Takara). For bacterial diversity analysis, V3-V4 (or V4-V5) variable regions of 16S rRNA genes was amplified with universal primers 343 F and 798 R