GSM8968769: Hela S3 clone2 Metaphase biol rep1; Homo sapiens; OTHER
Cleaved genomic DNA was extracted from the rinsed cell pellets using proteinase K digestion followed by phenol/chloroform extraction, ethanol precipitation. The purified DNA was resolved on a 1.8% NuSieve 3:1 (Lonza) agarose gel. DNA fragments (~100 to 300 bp) were gel-purified and prepared for paired-end sequencing NEBNext Ultra II DNA Lib prep