DNA-Seq of Polyrhizophydium stewartii JEL0888
Organism was grown in liquid medium and pelleted material after 1-2 weeks followed by 2X CTAB extraction. The library was sequencing on a on a FLO-MIN106 R9.4.1  flowcell and NBD104 barcoding kit and LSK109 ligation sequencing kit. basecalling and demuliplexing was performed with guppy 6.3.8; Additional reads were rescued from the unclassified barcoding step by mapping to an assembled genome from Illumina data alone.