GSM8488894: BICR10_shControl_H3K27Cr_2; Homo sapiens; ChIP-Seq
For YEATS2 ChIP-seq, chromatin extracted from BICR10 cells was used. For H3K27Cr ChIP-seq, chromatin extracted from either shControl or shYEATS2 BICR10 cells was used. ChIP was performed as described previously (Shukla et al., 2011; Khoury et al., 2020) Library was prepared using TruSeq ChIP Library Preparation Kit (IP-202-1012) for YEATS2 ChIP-seq and NEBNext Ultra II DNA lib preparation for Illumina Kit (NEBNext #E7645S) for H3K27Cr ChIP-seq. 100 bp (for YEATS2 ChIP-seq) or 150 bp (H3K27Cr ChIP-seq) paired-end sequencing was done using Illumina NovaSeq 6000.