Sabino Canyon 5
Genomic DNA was extracted using a modified CTAB protocol. DNA samples were used for preparation of sequence-based genotyping (SBG) libraries based on a double-digest RADseq (ddRAD) protocol. Libraries were constructed using restriction enzymes such as PstI, EcoRI, or SbfI (rare cutters), in combination with MseI, MspI, or similar frequent cutters, producing an estimated 50,000 to 150,000 fragments per sample. Following enzymatic digestion, barcoded adapters were ligated to individual samples, which were then pooled in multiplexed batches of 95. Libraries underwent size selection, PCR amplification, and purification. Sequencing was performed on an Illumina NovaSeq 6000 platform using a single-end 1100 bp configuration. Raw reads were delivered in FASTQ format, with each project allocated to a full SP flow cell lane, yielding 325400 million reads passing filter and ?80% bases at Q30 or higher.