GSM9111099: MDA-F4-2; Homo sapiens; RNA-Seq
RNA was harvested using Rneasy mini plus kit (Qiagen). 1 ug pf total RNA was used for the construction of sequencing libraries. 1. Ribosomal RNA depletion; 2. RNA Fragmentation and random priming; 3. First and second strand cDNA synthesis; 4. End repair, 5'Phosphorylation, and dA-Tailing; 5. Adaptor ligation, PCR enrichment, and sequencing