Metagenome of gut of Bemisia tabaci
Shotgun metagenomic sequencing libraries were prepared from total community genomic DNA using the KAPA HyperPlus Library Preparation Kit (Roche). Genomic DNA was enzymatically fragmented, followed by end repair, A-tailing, adapter ligation, and limited-cycle PCR amplification to generate sequencing-ready libraries. Library quality and concentration were assessed using Qubit fluorometry and Agilent TapeStation analysis. Libraries were constructed without targeted enrichment, representing random sampling of metagenomic DNA.