mtDNA-seq of seawater metagenome: seawater from Pond Inlet
Environmental DNA (eDNA) was extracted from bottled drinking water, which was used as field blanks for samples collected at multiple Arctic sites to assess biodiversity and community structure in marine ecosystems. Drinking water was poured into a cleaned sampling Niskin bottle at each sampling site. DNA was extracted using the DNeasy PowerWater Kit (Qiagen) and sequenced on the Illumina MiSeq platform for metabarcoding analysis targeting the COI gene region. The goal of this study is to monitor spatial variation in Arctic macro-organismal communities and to establish a comprehensive portrait of Arctic biodiversity using eDNA metabarcoding.