GSM9864719: BY4741_Δsnr31Δsnr54_noDMS_2; Saccharomyces cerevisiae; OTHER
RNA was purified by phenol_chloroform extraction followed by ethanol precipitation. RNA was fragmented, size_selected by denaturing polyacrylamide gel electrophoresis, dephosphorylated with T4 polynucleotide kinase, ligated to a pre_adenylated 3′ DNA adapter using truncated T4 RNA ligase 2 (K227Q), reverse transcribed using TGIRT_III reverse transcriptase, circularized with CircLigase II, amplified by indexed PCR, size_selected by native polyacrylamide gel electrophoresis, and quantified prior to sequencing. Libraries were sequenced on an Illumina NovaSeq X platform using single_end 100_bp reads.