Draft genome sequence of Enterobacter hormaechei HKEH-1, isolated from a blood samplefrom a type II diabetic patient
A bacterial isolate was recovered from the blood of a type II diabetic patient and initially cultured on blood agar at 37 C for 1824 hours. Based on phenotypic traits, it was presumptively identified as Acinetobacter baumannii. The isolate was sent to the Department of Medical Microbiology, University of South China, for molecular analysis. Sub-culturing on MacConkey agar yielded pink colonies, indicating lactose fermentation, a trait inconsistent with the expected non-lactose fermenting profile of A. baumannii (7, 8). To resolve this discrepancy, BLAST analysis of the 16S rDNA sequence identified theorganism as Enterobacter hormaechei, correcting the initial misidentification. Genomic DNA was sheared to an average fragment size of 350 bp using the Covaris LE220R-plus system (Covaris, USA). Fragmented DNA was subsequently end-repaired, A-tailed, and ligated to full-length Illumina sequencing adapters.