Switchgrass soil
We first extracted DNA from ~0.5 g of soil using the DNeasy PowerSoil Pro Kit (Qiagen, Germantown, MD).  Amplicon libraries were prepared using the SMRTbell Libraries using PacBio barcoded overhang adapters kit, following manufacturers recommendations. DNA was sequenced at the Biotechnology Center of the University of Wisconsin-Madison on a PacBio Sequel II machine.