RNA Seq of Homo sapiens : 01021B_DB_20221122
DNA were extracted from stool samples using the MagAttract PowerMicrobiome kit (Qiagen) on a STARlet robotic platform (Hamilton). PCR amplification targeted the V3-V4 regions of 16S rRNA gene ((PCR_341 F: 5'-CCTACGGGNGGCWGCAG-3'); (PCR_785R: 5'-GACTACHVGGGTATCTAATCC-3')).