amplicon sequencing of E. amylovora: Ea273+E2002a+E4001a_T3_R1
DNA was extracted from E. amylovora cultures (single strain or mixed). Universal Illumina adaptors were added to primers that flank diagnostic regions of the genome. These primers were used in a multiplexed PCR reaction, which amplified the region (amplicon) of interest.  These amplicons were then sequenced on the Illumina MiSeq platform.