GSM9081600: Young uPAR 1; Mus musculus; RNA-Seq
RNA was harvested using Rneasy mini plus kit (Qiagen). 1 ug of total RNA was used for construction of sequencing libraries. Libraries were prepared using the KAPA mRNA HyperPrep Kit (Roche, Cat# KK8581), following the manufacturer's standard protocol with 120 ng of total RNA per sample. mRNA was captured using oligo(dT) magnetic beads, fragmented (6 minutes at 85°C, aiming for 300–400 bp), and reverse transcribed into cDNA. After end repair, A-tailing, and adapter ligation, libraries were PCR-amplified (13 cycles), then purified and QCed using TapeStation and Qubit before pooling and sequencing. Libraries were sequenced on an Illumina NextSeq 2000 using a P3 flowcell with paired-end 150 bp reads (PE150), using NextSeq2000 Control Software was v1.5.0 ( release Nov 15, 2022).