GSM9111657: IORT_Sample_21; Homo sapiens; RNA-Seq
Cryopreserved immune cell samples were thawed, washed with PBS, and processed using the NucleoSpin miRNA kit (Macherey & Nagel, Cat. No. 740971) following the manufacturer's instructions for total RNA extraction (cell lysis in buffer ML, no DNA/protein isolation). RNA was eluted in 50 µL nuclease-free water and quantified via NanoDrop. RNA libraries were constructed using the QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the manufacturer's instructions. Sequencing was performed by Novogene (Cambridge, UK).