GSM9794248: SSc_1; Homo sapiens; OTHER
Biopsy specimens were processed for 10x Chromium 3′ single-cell RNA sequencing either immediately after tissue dissociation or following cryopreservation in CryoStor® CS10. Adjacent biopsy specimens were formalin-fixed and paraffin-embedded (FFPE) for spatial transcriptomics and hematoxylin and eosin (H&E) staining. A subset of the FFPE-preserved tissues was additionally analyzed using 10x Genomics Flex single-cell RNA sequencing. Spatial transcriptomics was performed using the Visium v2 Whole Transcriptome (WT) Panel Spatial Gene Expression workflow (55-µm spot diameter). RNA quality was assessed from scrolls generated from each FFPE block. Blocks with a percentage of RNA fragments >200 nucleotides (DV200) of at least 30% were selected for spatial transcriptomics. Tissue sections were mounted on Visium slides and processed in Visium v2 cassettes for library preparation. Spatially barcoded libraries were generated and sequenced according to the manufacturer's protocols.