GSM9687012: P126BK TCRseq; Homo sapiens; OTHER
Total RNA was extracted from facs sorted CD45+ or CD3+ cells from human kidney (and blood) . Single‑cell TCR sequencing libraries were generated using the Chromium Single Cell 5ʹ Library & Gel Bead Kit, 10x Genomics and the Chromium Single Cell V(D)J Reagent Kits humanT cell ccording to the manufacturer's instructions. Briefly, single T cells were captured into droplets, reverse‑transcribed, amplified, and then TCR‑specific cDNA was enriched by targeted PCR using V(D)J enrichment primers. The resulting TCR‑enriched libraries were normalized, pooled, and sequenced on an Illumina  using a paired‑end [bp] run configuration.