Progreso pustular microbial mats
The genomic DNA was subjected to random fragmentation. The resulting fragments were end-repaired, had an adenine (A) tail added, and were subsequently ligated with Illumina adapters. The resulting adapter-ligated fragments were size-selected, amplified by PCR, and purified. The libraries were quantified using Qubit and qPCR. Subsequently, they were pooled and sequenced on the Illumina NovaSeq X Series system (2  150 bp paired-end reads).