Single-cell transcriptional landscape of human embryonic limb development - Visium
Single-cell transcriptional landscape of human embryonic limb development - Visium
The human embryonic samples were collected from medical abortions. The samples were stored in hibernate E medium (Thermo Fisher A1370601) and transported to the research lab for dissection. The limb samples were stored in HypoThermosol at 4 degrees, then were embedded and frozen in OCT on isopentane-dry ice slurry. Nucleic acid was extracted according to the standard protocol of the Spatial 3' v1 protocol Tissue sections were loaded onto Visium chips according to the standard protocol of the Spatial 3' v1 protocol