Leaf (CRX1298605)
High-quality total RNA was extracted using a plant total RNA isolation kit (Sangon Biotech Co., China). After quality control, the RNA samples were subjected to mRNA enrichment with dT probes and fragmented, and the first and second cDNA strands were synthesized. Sequencing adapters were ligated to the double-stranded DNA fragments, and amplified by PCR. The constructed library was then sequenced on an Illumina Nextseq 2000 platform.