GSM9437861: Day5_KO_1; Homo sapiens; RNA-Seq
Differentiated cells collected on days 2, 5, and 15 were dissociated using Accutase and neutralized with fetal bovine serum in DMEM. For each time point, three biological replicates were obtained from both WT and KO groups. After centrifugation at 1,000 g for 5 min, cell pellets were resuspended in DPBS containing 0.04% bovine serum albumin and immediately submitted for scRNA-seq. Single-cell libraries were generated using the Chromium GEM-X Single Cell 3′ Kit v4 (10x Genomics) according to the manufacturer's instructions, and sequenced on an Illumina nova X to a mean depth of at least 20K reads per cell.