WRKY14_Input (CRX1857998; CRA027928)
Genomic DNA library was constructed from tomato (Solanum lycopersicum) using the NEBNext DNA Library Prep Master Mix Set for Illumina. Recombinant SlWRKY14-HaloTag protein was expressed in a wheat germ system and immobilized on HaloTag beads. Beads were incubated with the DNA library in PBS with 0.005% IGEPAL at 4 C for 2 h, washed five times with PBS, and bound DNA was eluted in 50 mM Tris-HCl (pH 8.5). Libraries were quantified with a KAPA Library Quantification Kit and sequenced on Illumina NovaSeq 6000 (150 bp paired-end).