GSM8944533: ESC2i-rep2; Mus musculus; RNA-Seq
ESCs cultured in different medium were trypsinized and dissociated into the single-cell. Total RNA was extracted using the GeneJET RNA purification kit. For RNA-seq library preparation, 1 g total RNA was used to prepare the RNA-seq library with the Truseq RNA Library Prep Kit V2 with RiboZero (Illumina). For RNA-seq library preparation, 1 g total RNA was used to prepare the RNA-seq library with the Truseq RNA Library Prep Kit V2 with RiboZero (Illumina).