GSM9000230: C_3; Mus musculus; RNA-Seq
For transcriptomic analysis, RNA was extracted from frozen RP tumors using NucleoSpin RNA kit (Macherey-Nagel) according to manufacturer's instructions. After quality control with Tape Station (Agilent), samples were sequenced by Macrogen. Truseq mRNA Library NovaSeq6000 150PE (150 x 2 bp) with 30 M total reads/sample was built. Sequencing consisted of readings of a hundred and fifty base pairs in 5'-3' direction and vice versa.