V3-V4 16S Hose Biofilm Nods Control 52 weeks 1
DNA was extracted from 500ul of biofilm using the FastDNA SPIN kit (MP Biomedicals); Preparation of Illumina Nextera barcode, two-step PCR libraries (V34) of 20 and 10 cycles; Sequencing on Illumina MiSeq, v2, 2x250bp; Demultiplexing and trimming of Illumina adaptor residuals