GSM8797382: induction, day 10, time 48, litter 1; Rattus norvegicus; RNA-Seq
RNA isolated via intital wash with corning cell recovery solution to remove excess matrigel, followed by lysis and disruption with Qiagen RLT buffer. Lyzed samples stored at -80 until analysis. Lyzed samples sento to Novogene for isolation and sequencing using totalRNA  isolation and messenger RNA purification using poly-T oligo-attached magnetic beads. mRNA was then fragmented and the first strand cDNA was synthesized using random hexamer primers, followed by second strand cDNA synthesis using dTTP for nondirectional library, and sequenced by a NovaSEq 6000 system