RNA-Seq of mus musclus: adult male Liver
1 g of total RNA was used as input to prepare libraries using the Illumina Stranded Total RNA Prep with Ribo-Zero Plus (Illumina, San Diego, CA, USA). This method was employed to deplete ribosomal RNA while maintaining strand-specific information. Library construction and high-throughput sequencing were conducted at the Korea National Institute of Health (KNIH, Cheongju, South Korea). Library quantity and quality were assessed and paired-end sequencing (100 read length) was performed on a NovaSeq 6000 system (Illumina).