sequence of bacteria
Soil genomic DNA was extracted using the MP-Fast DNA Spin Kit for Soil. The V3-V5 hypervariable region of the bacterial 16S rRNA gene was amplified using the specific primers 515F (5'-GTGCCAGCMGCCGG-3') and 907R (5'-CCGTCAATTCMTTTRTT-3') with TransStart FastPfu DNA Polymerase. PCR products were purified using AMPure PB beads and quantified with the QuantiFluor-ST system. Sequencing libraries were generated using the TruSeq DNA Sample Prep Kit following the manufacturer's recommendations. The final library was sequenced on an Illumina MiSeq platform.