GSM8577511: FB_PLVX_5; Homo sapiens; RNA-Seq
Treated cell lines were seeded in 100 mm tissue culture dishes (Corning, NY, USA, Cat. 430167) at a density of 3x106 and incubated under previous conditions overnight. Subsequently,  the cells were harvested for RNA extraction using the column-based extraction and purification system Zymo Quick-RNA MiniPrep (Zymo Research Corp., Irvine, CA, USA) following manufacturer's instructions. The obtained RNA was quantified by absorbance at 260/280 nm and stored at -80°C until further use. First, total RNA is fragmented into 150 pb segmentes. Second, Reverse transcription is achive to obtain cDNA. Third, ligation of adapters at the ends.