GSM9080845: 150nc-2; Homo sapiens; RNA-Seq
Total RNA was extracted from EC cells using TRIzol™ reagent, with procedures performed in strict accordance with the manufacturer's guidelines. Reverse transcription of 1 μg mRNA into cDNA was performed using the Evo M-MLVRT Mix Kit Ver.2 The Illumina standard protocol (VAHTS General V6 RNA Sequencing Library Preparation Kit) was used for library preparation. The cDNA library concentration and size distribution were evaluated using the Agilent 4200 bioanalyzer, followed by sequencing using the Illumina novaseq6000.