GSM9214642: RNA-seq, Wt, mature CD8 SP thymocytes, rep.1; Mus musculus; RNA-Seq
Total RNA was extracted from FACS-purified mature CD8 SP thymocytes using RNeasy Mini kit (Qiagen). RNA integrity number was evaluated using an Agilent 2100 Bioanalyzer with the RNA 6000 Pico Kit (Agiletn), and samples with RNA integrity number > 8 were used for library preparation. RNA-seq libraries were constructed from 10 ng of total RNA using the NEBNext RNA Library Prep Kit for Illumina (NEB).