NGS of qPCR amplicon of D. fragilis diagnostic assay
Amplified using forward primer sequence Df-124F (5-CAACGGATGTCTTGGCTCTTTA-3) and reverse primer sequence Df-221R (5-TGCATTCAAAGATCGAACTTATCAC-3) from lab based qPCR assay. Expected product size 50bp. qPCR assay is designed to detect Dientamoeba fragilis. NGS data was generated to check specificity of the qPCR assay.