RNA-Seq
For the library construction, we used a total RNA input of 10 ng, following the guidelines provided by the manufacturer. Briefly, RNA was reverse transcribed using the SuperScript VILO cDNA Synthesis Kit. Libraries were prepared manually using the Ion AmpliSeqTM Library Kit 2.0 for the amplification of the targeted genes and the IonXpressTM Barcode Adapters  for targeted enrichment and barcoding. Library purification and size selection were performed using the AMPure XP beads. The final quantification of the libraries was conducted with the Ion Library TaqMan Quantitation Kit.