Whole genome resequencing of Asian shrew moles
Briefly, genomic DNA samples were fragmented by sonication to a size of 350 bp. The DNA fragments were subjected to end polishing, A-tailing, and ligation with a full-length adapter for Illumina sequencing before additional PCR (polymerase chain reaction) amplification. The PCR products were then purified using the AMPure XP system (Beverly, USA). We subsequently assessed library quality using the Agilent 5400 system (Agilent, USA) and quantified it through QPCR (1.5 nM).