GSM9140680: iWAT MondoAKO High Fat Diet 22; Mus musculus; RNA-Seq
RNA isolation and qRT-PCR. Total RNA was isolated from frozen tissues or cells using the RNeasy Mini Kit (QIAGEN) and treated with RNase-Free DNase Set (QIAGEN) per manufacturer's instructions. cDNA was synthesized using the Affinity Script cDNA Synthesis Kit (Agilent Technologies) or High-Capacity cDNA Reverse Transcription Kit (ThermoFisher Scientific #4368814) with 0.25 μg total RNA. RNA-Seq library preparation, sequencing and analysis. GENEWIZ, LLC. (South Plainfield, NJ) performed RNA library preparations and sequencing reactions per standard operating procedure [16, 17].