GSM8581599: PD19005; Homo sapiens; RNA-Seq
RNA extraction was performed with QIAGen miRNAeasy mini kit in combination with DNAse treatment according to the manufacturers instruction. RNA was processed using the previously described Celseq2 technique adapted for bulk sequencing. In brief, reverse transcription and second strand synthesis were performed in seperate tubes; libraries were pooled for in vitro transcription based RNA amplification. Libraries were generate using Truseq small RNA primer (rpi) library primers, Libraries were sequenced on an Illumina NextSeq500 using 75bp paired end sequencing.