mtDNA-seq of extraction blank metagenome
Environmental DNA (eDNA) was extracted using clean filters, used as extraction blanks. DNA was extracted using the DNeasy PowerWater Kit (Qiagen) and sequenced on the Illumina MiSeq platform for metabarcoding analysis targeting the COI gene region. The goal of this study is to monitor spatial variation in Arctic macro-organismal communities and to establish a comprehensive portrait of Arctic biodiversity using eDNA metabarcoding.