16S rDNA lettuce rhizosphere soil(control group S2-1) (CRX1892472)
The bacterial 16S rDNA V3-V4 region was amplified via PCR using primers 338F and 806R. Sequencing libraries were then constructed from the purified amplicons following the standard workflow of the ALFA-SEQ DNA Library Prep Kit. The size distribution of the resulting libraries was assessed using the Qsep400 High-throughput Nucleic Acid and Protein Analysis System, and library concentration was measured using a Qubit 4.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA).