235 3d-2 (CRX1830375; CRA027592)
When 14 japonica rice varieties were subjected to low-temperature treatment at the booting stage for 0 d and 3 d, young panicles were collected at different time points for transcriptome analysis. The harvested samples were wrapped in sterilized tin foil, flash-frozen in liquid nitrogen, and then stored at -80 C. Total RNA was extracted using TRarsZolUpRNA Kit (Beijing, China). Firstly, the quality control of 14 samples was measured by OneDrop 1000 + instrument. The RNA content and A260 / A280 were measured by OneDrop 1000 + instrument. Three complete target bands were detected by agarose gel electrophoresis. Secondly, the samples were sent to the sequencing company for testing, and the qualified samples were used to construct the library. The clean reads at different temperatures were compared with the control genome by HISAT2 technology (Kim et al. 2019), and the number of genes was obtained by FeatureCounts analysis. Finally, the FPKM values of each gene were calculated by gene length, read counts and other methods to reflect the expression level of the gene.