RNA-Seq of Homo sapiens HCT116 cells: shControl, rep2
The sequencing data was filtered with SOAPnuke (v1.6.5) by (1) Removing reads containing sequencing adapter; (2) Removing reads whose low-quality base ratio (base quality less than or equal to 15) is more than 20%; (3) Removing reads whose unknown base ('N' base) ratio is more than 5%, afterwards clean reads were obtained and stored in FASTQ format. The clean data were mapped to the GRCh38/hg38 by HISAT (v2.2.1) , then were mapped to the assembled unique gene by Bowtie2 (v2.4.5). The expression level of genes was calculated by RSEM (v1.3.2)