NextSeq 2000 sequencing
We used RNA sequencing analyze the transcriptomic profiles of NK cells in 3 different conditions (Tumor free condition and tumor bearing mice with diffuse large B cell lymphoma disseminated either through the lymphatic system or blood vessels)
NEBNext Ultraexpress�RNA Library Prep Kit for Illumina (E3330S - New England BioLabs�Inc.) was used with the NEBNext�Multiplex Oligos for Illumina (New England BioLabs�Inc.) and NEBNext��Poly(A) mRNA Magnetic Isolation Module for cDNA synthesis with the addition of barcode sequences. The pre-pool sequencing was performed using the NextSeq2000 (Illumina, San Diego, CA, USA) with the P2 reagents kit V3 (100cycles; Illumina).�Samples were processed starting from stranded, single-ended 120bp-long sequencing reads.