genome skimming library P. caribaeorum
Library preparation was carried out using the NEBNext Multiplex Oligos adaptor kit at the National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan. DNA concentration after library preparation was quantified using a Qubit dsDNA HS assay kit on a Qubit 4.0 Fluorometer (Thermo Fisher Scientific). Libraries were amplified with 10 cycles of PCR, pooled, and sequenced on an Illumina NovaSeq X Plus platform using paired-end 150 bp reads. Low-coverage whole-genome sequencing (genome skimming) was performed, and ultraconserved element (UCE) loci were recovered through downstream bioinformatic analyses.