"RNA-seq of MOLM-14 cells treated with Brusatol for 24 hours, replicate 1"
"Total RNA (1_ug) was used for library preparation. Poly(A) mRNA was isolated using Oligo(dT) beads, followed by fragmentation with divalent cations and high temperature. First- and second-strand cDNA synthesis was performed using random primers. After end repair and A-tailing, sequencing adapters were ligated. Size selection and PCR amplification were performed, and final libraries were validated. "