GSM9687047: 4T1_CUT&RUN_PHF10-sgPB-biol rep1; Mus musculus; OTHER
Cut&Run was done according to the protocol available from EpiCypher (v2.2) with minor modifications- in-house prepared pAG-MNase was used and incubation of cells with pAG-MNase in section IV was done at 4C for 1h. Amount of pAG-MNase used was 37ng/reaction. Antibodies used were PHF10 (Invitrogen #PA5-30678), H3K4me3 (EpiCypher 13-0060), Pbrm1 (Bethyl # A301-591A), and Rabbit control IgG (EpiCypher # 13-0042) Libraries were prepared using the CUTANA™ CUT&RUN Library Prep Kit according to the manufacturer's instructions, and quality checked using Qubit and Agilent TapeStation by Purdue Genomics Core Facility. Libraries were sequenced using 150bp PE sequencing on Aviti platform (Element Biosciences) by the Purdue Genomics Core Facility. All experiments were performed with two biological replicates.