GSM9472916: Mouse heart single cell RNA-seq under NFD or HFD; Mus musculus; RNA-Seq
Whole hearts were harvested and enzymatically dissociated into single-cell suspensions according to standard protocols. Cells were filtered to remove debris, counted, and assessed for viability prior to single-cell RNA sequencing library preparation. Single-cell RNA sequencing libraries were prepared using the Evercode Whole Transcriptome Mini v3 kit (Parse Biosciences) following the manufacturer's protocol. Cells were barcoded using Parse Biosciences' combinatorial barcoding strategy, and libraries were amplified and prepared for paired-end sequencing.