GSM9214545: H-HIF_2-Hypoxia; Homo sapiens; OTHER
After corresponding treatment, HUVEC at a density of 10^5 cells were collected. HUVEC at a density of 10^5 cells were bound to magnetic beads, and digitonin was added to penetrate the cell membrane. Then the anti-HIF2α antibody, ChiTag goat anti-rabbit IgG antibody, and pAG-Tn5 were desiccated and segmented successively. The reaction products were collected by DNA Clean Beads and enriched by PCR to construct the next-generation sequencing library.