RNA-Seq of Strongylocentrotus purpuratus coelomocytes: 6 hours post-infection with Vibrio diazotrophicus
Coelomocytes were extracted from adult animals by inserting a syringe into the peristomial membrane.  Whole coelomic fluid was diluted 1:1 in calcium/magnesium-free sea water. Coelomocytes were pelleted and RNA was extracted.