GSM8563934: 58492_WB5; Drosophila melanogaster; RNA-Seq
Total RNA was extracted from adult five-day-old flies or ovaries from control 58492 and knockout strains using guanidine isothiocyanate RNAzol RT (Molecular Research Center, USA) following the manufacturer's protocol. Libraries for RNA-seq were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, USA) according to the manufacturer's guidelines