ATAC-seq for Linear RAG Scanning Mediates Editing of Igκ Variable Region Repertoires
ATAC-seq for Linear RAG Scanning Mediates Editing of Igκ Variable Region Repertoires
50,000 G1-arrested RAG-deficient v-Abl cells were used for experiment v-Abl cell lines were cultured in RPMI1640 media with 15% FBS G1-arrest was performed by treating v-Abl cell lines with 3uM STI for 4 days Briefly, the collected G1-arrest live cells were used for cell lysis, nuclei preparation, tagmentation by Tn5 and library amplification according to Zymo-Seq ATAC Library Kit (D5458). Libraries were PCR-amplified with Illumina primers and subjected to sequencing.