GSM9317390: human cExNK cells, Day 14, replicate 3; Homo sapiens; RNA-Seq
Total RNA was extracted by TRIzol Reagent (Thermo) Total RNA was constructed library using using VAHTS Universal V10 RNA-seq Library Prep Kit for MGI (Vazyme, NRM606). The libraries were size-selected for cDNA fragments of 200–300 bp on 2% Low Range Ultra Agarose and then subjected to PCR amplification using Phusion DNA polymerase (NEB) for 15 PCR cycles. Following quantification using TBS380, the paired-end libraries were sequenced