GSM8693478: P5751 scRNA-seq library; Homo sapiens; RNA-Seq
Samples were were dissociated using the Miltenyi Human Tumor Dissociation Kit, cleaned with ACK RBC lysis, and sorted with CD45+ beads. Cells were loaded onto the 10X chip with a ratio of 70% CD45- and 30% CD45+ cells cDNA libraries were prepared according to the 10X Chromium Next GEM Single Cell 3' Reagent Kits v3.1 protocol.