O. niloticus gut metagenome BR2
Genomic DNA was extracted from gut samples using the Wizard Genomic DNA Purification Kit. The V3V4 hypervariable regions of the bacterial 16S rRNA gene was amplified using the universal primer pair Bakt_341F (5-CCTACGGGNGGCWGCAG-3) and Bakt_805R (5-GACTACHVGGGTATCTAATCC-3) and sequenced on an Illumina MiSeq platform using paired-end sequencing.