NextSeq 550 sequencing
The RFP libraries were prepared as previously described in PMID: 22836135, without harringtonine treatment. Trizol (Invitrogen) was used to isolate total RNA, following manufacturerâ€™s instructions. Quick gel extraction of RNA from polyacrylamide gels was performed followed by overnight extraction at -80oC. Depletion of rRNA was not performed and rRNA was afterwards removed during data processing.