GSM8552296: B175_12h_3; Setaria italica; RNA-Seq
Total RNA was extracted using the TRIzol reagent (Invitrogen, CA, USA) according to the manufacturer's protocol.RNA purity and quantification were evaluated using the NanoDrop 2000  spectrophotometer (Thermo Scientific, USA). RNA integrity was assessed using the Agilent 2100  Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Then the libraries were constructed using  VAHTS Universal V6 RNA-seq Library Prep Kit according to the manufacturer's instructions. The  transcriptome sequencing and analysis were conducted by OE Biotech Co., Ltd. (Shanghai, China).