GSM8465575: spleen_sick, ADT; Mus musculus; OTHER
PCs from those tissues were enriched using anti-CD138 magnetic beads followed by cell sorting. Single cell suspension were then applied to the 10X Genomics platform using the Single Cell 5' Library & Gel Bead Kit (v1.1, single index) following the manufacturer's instructions. The purified cDNA was amplified and then used for 5' gene expression (GEX), CITE-Seq and BCR library preparation using the Single Cell 5' Library & Gel Bead Kit (v1.1, 10x Genomics). BCR target enrichment was achieved using the Chromium Single Cell V(D)J Enrichment Kit. Through fragmentation, adapter ligation and index PCR the final GEX and BCR libraries were obtained. The CITE-Seq libraries were obtained only by index PCR. The quality of single cell 5' GEX, CITE-Seq and BCR libraries was assessed by Qubit quantification and Bioanalyzer fragment analysis (HS DNA Kit, Agilent). The sequencing was performed on a NextSeq2000 device (Illumina) using NextSeq 1000/2000 P3 reagent kits (200 Cycles, Illumina) with the recommended sequencing conditions for 5' GEX and Cite-Seq libraries (read1: 26nt, read2: 98nt, index1: 8nt, index2: 0) and NextSeq 1000/2000 P3 reagent kits (300 Cycles, Illumina) for BCR libraries (read1: 151nt, read2: 151nt, index1: 8nt, index2: 0, 20% PhiX spike-in).