GSM8552281: Cells_PMA 20ng/ml_ inomycin 500 ng/m_24hrs, replicate 3; Homo sapiens; RNA-Seq
The total RNAs of treated Jurkat cells were isolated by using the QIAamp RNA Blood Mini Kit (QIAGEN GmbH, Germany) The SureSelect XT HS2 mRNA Library Preparation kit (Agilent, Santa Clara, CA, USA) was used for Circular RNA/ RNA IP samples to construct nonpoly-A select step RNA libraries, followed by HighPrep PCR clean-up system. Input of 10 ul Circular RNA/ RNA IP sample was directly mixed with 10 ul priming buffer in enzymatic fragmentation steps and subsequently then subjected to cDNA construction. After adaptor ligation, unique dual indexes were introduced and each library was amplified in a PCR with 10 cycles. Library quality and quantity Final libraries were quantified using Nanodrop,Qubit dsDNA HS Assay and Agilent 4200 TapeStation System.