GSM9028690: SP_12_ON_DOX; Mus musculus; RNA-Seq
Total RNA was extracted from the splenic leukemia cells in BM transplanted mice using Trizol reagent (Invitrogen, 15596026), according to the manufacturer's protocol. Whole RNA was treated with DNaseI for 30 min; and 500 ng RNA was used to prepare 3'mRNA-Seq libraries with the QuantSeq 3'mRNA-Seq V2 Library Prep Kit FWD with UDI (UDI12A_0001-0096; Lexogen) for Illumina according to manufacturer protocol