CRISPR Screen Targeting Mouse Protein Tyrosine Phosphatases
Genomic DNA with sgRNA insertions was amplified by PCR using primers TCTTGTGGAAAGGACGAAACACCG and TCTACTATTCTTTCCCCTGCACTGT. The PCR products were end-repaired, A-overhangs were added, and barcodes were ligated. Libraries were sequenced using the MiSeq platform with MiSeq Reagent Kit v2