GSM8967927: old, 5-week; Mus musculus; RNA-Seq
We pooled retinas and digested tissue in papain solution (125 units/ml) containing protease & phosphatase inhibitor and DNAse I (5,000 units/ml). After 40 min digestion at 4C, we neutralized the reaction using ovomucoid solution. Then, we enriched the single cell suspension for CD45-positive cells using the EasySep Mouse CD45 Positive Selection Kit following the manufacturer's recommended protocol. Finally, we washed and re-suspended the cells in DMEM media containing 10% fetal bovine serum and used them as input for single cell RNA sequencing on the 10x Genomics platform. We used the Chromium Single Cell 3'v3.1 Reagent Kit according to the manufacturer's protocol.