GSM8582139: YY1_17; Mus musculus; OTHER
B6 Lin- fetal liver cells were extracted using the EasySep ™ magnet system (StemCell™ Technologies) with antibodies against CD3, CD4, CD5, CD8, B220, CD19, IgM, CD127, AA4.1 and Ter119 (eBioscience). Around 300,000 cells were used for each CUT&Tag reaction with antibodies against H3K4me3 (Abcam ab213224) and YY1 (Active Motif 61780). The library was constructed following the published protocol (Li, Yuefeng et al. “Chromatin and transcription factor profiling in rare stem cell populations using CUT&Tag.” STAR protocols vol. 2,3 100751. 19 Aug. 2021). The library is amplified with barcoded i5 and i7 primer using NEBNext High-Fidelity 2X PCR Master Mix (M0541). The quality of the tagmented DNA library is assessed using Bioanalyzer High Sensitivity DNA Analysis (Agilent).