Viral metagenomics of raptor cloacal swab sample
Cloacal swabs were collected and stored in UTM buffer. 200ul of UTM buffer from each swab sample was used to extract viral nucleic acid with a High Pure Viral Nucleic acid kit (Roche Diagnostics, USA). 1ul of viral nucleic acid was utilized in a rolling circle amplification (RCA) reaction using the enzyme phi29 (Watchmaker Genomics, USA). For each sample 15ul of viral nucleic acid was combined with 15ul of RCA and used to construct DNA sequencing libraries using an Illumina DNA library preparation (M) tagmentation kit (Illumina, USA). These libraries were sequenced on an Illumina NovaSeq X plus sequencer (Illumina, USA) at Psomagen Inc. (USA).