GSM9652693: THP-1 cells, control siRNA, TNF-α, 24 h, biological replicate 3; Homo sapiens; RNA-Seq
Total RNA was isolated from treated THP-1 cells after 24 hours using the RNeasy Mini Kit (Qiagen). RNA-seq libraries were prepared from total RNA using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) according to the manufacturer's protocol. All samples are of excellent quality (RIN/RQN > 7) and have sufficient RNA (> 100 ng) to proceed to mRNA Stranded Library prep.