GSM9170558: SaB200uM1; Homo sapiens; RNA-Seq
Total RNA was extracted from A375 cells using TRIzol reagent (Invitrogen) according to the manufacturer's instructions. RNA libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB) following the manufacturer's instructions. Briefly, mRNA was enriched using poly-T oligo-attached magnetic beads, fragmented, reverse transcribed into cDNA, end repaired, A-tailed, ligated with sequencing adapters, and PCR amplified to generate the final cDNA libraries.