sRNA-seq of wheat anther
Total RNA was extracted from anthers using FsatPure Universal Plant Total RNA Isolation Kit (Vazyme, China), Libraries were constructed with the Vazyme miRNA 1st Strand cDNA Synthesis Kit (Vazyme, China). The procedure was performed following the kit's instructions. Firstly, using Poly(A) polymerase to add an A-tail to the 3' end of the miRNA. Subsequently, a universal reverse transcription primer is employed to generate the first-strand cDNA of the miRNA through reverse transcription. Four libraries cDNA reaction mixture were sent to Shanghai Mindray Biotechnology Co., Ltd. for Illumina sequencing on the HiSeq2500 platform. Each with a sequencing depth of 10 Mb raw reads.