GSM8833446: sample 1 of liver constituent cells from a FALD liver; Homo sapiens; RNA-Seq
Frozen surgical liver tissue was cut into pieces approximately 1 mm3, fixed with formalin using a Chromium Next GEM Single-Cell Fixed RNA Sample Preparation Kit (10x Genomics, Pleasanton, CA), and then dispersed into single cells using gentleMACS (Miltenyi Biotec) according to the manufacturer's instructions. For the construction of the scRNA-seq library, a Chromium Fixed RNA Kit, Human Transcriptome, 1BC (10x Genomics) was used according to the manufacturer's instructions. Libraries were sequenced on a DNBSEQ-G400‌RS (MGI Tech) at a read length of 28 × 90.