GSM8465632: Outer meniscus tissue 2; Sus scrofa; RNA-Seq
Tissues were pulverized in TRIzol under liquid nitrogen using a freezer mill. RNA was extracted by TRIzol/chloroform separation according to the manufacturer's protocol, then resuspended in lysis buffer and column purified according to the Norgen RNA extraction kit protocol (Catalog # 48300; Norgen Biotek Corp). Cell pellets were flash frozen in liquid nitrogen and stored at -80°C until RNA extraction was performed using the Norgen Total RNA Purification Plus extraction kit. KAPA Stranded mRNA-Seq Kit--KK8421