F_ALK_05 (CRX2105313)
Total genomic DNA was extracted from fecal samples using the E.Z.N.A. Soil DNA Kit (Omega Bio-tek, Norcross, GA, USA) following 1% agarose gel electrophoresis. The hypervariable V3-V4 region of the bacterial 16S rRNA gene was amplified in triplicate using universal primers. Finally, the amplicons were sequenced on the Illumina MiSeq platform in pair-end 254 bp mode.