GSM9651765: preLSC, WT, rep3; Mus musculus; RNA-Seq
Total RNA was extracted using QIAzol and teated with DNase I following QIAGEN RNA mini extraction kit's instructions. Libraries were prepared from 1 ug of total RNA using the NEBNext Ultra II Directional RNA Library Prep kit for Illumina (NEB, cat. no. E7760) with NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB, cat. no. E6310) following the manufacture's protocol. Eight cycles of PCR was performed to amplify libraries and the pooled library was sequenced on Illumina NextSeq 550 with 85 bp single-end.