WGS of barcoded B. theta isolate: experiment E1 mouse 10 at day 17, isolate #15
DNA was extracted from colony isolates (sampled from feces) using UltraClean 96 microbial kits [Qiagen]. DNA was added to a tagmentation reaction for 10 min at 55 C before neutralization. In a 10-cycle PCR, adapter regions with TruSeq unique dual indices [Illumina] were attached to permit pooled sequencing and demultiplexing. Clean up on pooled libraries was performed with Ampure XP Beads [Beckman Coulter] prior to sequencing.