Om young leaves
Total RNA was extracted from O. mungos leaves using the RNeasy Plant Mini Kit (Qiagen), followed by further purification and concentration with the RNA Clean & Concentrator-5 Kit (Zymo Research). All of these procedures were performed according to manufacturer's guidelines. RNA quality was assessed using an Implen NanoPhotometer N60. Two RNA samples, one from young leaves and another from mature leaves meeting quality thresholds required for sequencing (≥ 400 ng total RNA; A260/280 = 1.8-2.2; A260/230 ≥ 1.8), were submitted to Novogene, where sequencing, transcript assembly and annotation were performed following standard protocols