GSM8864840: Patient 3 months post treatment biological replicate 1; Homo sapiens; RNA-Seq
Patient n-of-1 trial and data collection was performed in accordance with protocols reviewed and approved by the WGC Institutional Review Board (IRB) (WCG IRB protocol #2001-01). Blood was collected in PAXgene tubes and shipped at room temperature to the sequencing facility (Genewiz, NJ) for processing within 24 hours for RNA sequencing. The RNA was extracted using the PAXgene RNA extraction kit. Barcoded libraries for RNA-seq were prepared with 100 ng of RNA using NEBNext Ultra II RNA Library Prep Kit (NEB, Ipswich, MA) for Illumina with QIAGEN QIAseq FastSelect -rRNA HMR Kit (QIAGEN, Germany) per manufacturer's protocol.