TIC+siV1 (CRX1511796; CRA022803)
Genomic DNA (2 ug) was digested with MspI for 16h. Following Illumina Pair-End protocol with modifications: DNA ends were repaired using T4 DNA polymerase/Klenow Fragment/T4 PNK, then adenylated with Klenow Fragment. Adaptors containing 5-methylcytosine were ligated via T4 DNA Ligase. Purification used QIAquick kits (Qiagen) and TrueMethyl Seq Kit magnetic beads. Oxidation involved adding 1uL CEGX oxidant to oxRRBS (1uL H2O to RRBS), incubated for 10min.Supernatant was transferred after centrifugation (14,000g,10min) for bisulfite conversion (TrueMethyl Seq Kit).