RNA-Seq of GBM8 cancer stem cell line
Total RNA was isolated from cells using the miRNeasy micro kit (Qiagen). Library preparation was performed using the rRNA depletion KAPA RNA HyperPrep Kit with RiboErase (HMR)(Roche Sequencing and Life Science). After the DNA libraries were cleaned, they were quantified using the Qubit dsDNA High Sensitivity Kit and run on the Agilent D100 ScreenTape Assay for the 4200 TapeStation System (Agilent, cat# 5067-5583). Samples were pooled and sequenced on  a NextSeq 500 using the 150 cycle, NextSeq 500/550 High Output v2 kit (Illumina, cat# FC-404-2002).