GSM8636219: Treatment, high mutation load kidney, replicate 3, RNA-seq; Mus musculus; RNA-Seq
Total RNA was isolated using the TRIzol method, and its concentration was quantified with the Qubit RNA XR Assay Kit (Thermo Fisher Scientific). RNA quality was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies). Sequencing libraries were prepared with the Illumina TruSeq RNA Preparation Kit. High-throughput sequencing was performed on a DNBSEQ platform, producing 150 bp paired-end reads, following the manufacturer's recommendations