IBD-free uninflamed intestinal mucosa microbiome. Colorectal cancer IBD-free control male individual C009, 73 years old.. Library batch 1.
16s rRNA V3 and V4 region amplification with specific primers as described in Kozich JJ, Westcott SL, Baxter NT, Highlander SK, Schloss PD. Development of a dual-index sequencing strategy and curation pipeline for analyzing amplicon sequence data on the MiSeq Illumina sequencing platform. Appl Environ Microbiol. 2013 Sep;79(17):5112-20. doi: 10.1128/AEM.01043-13. Epub 2013 Jun 21. PMID: 23793624; PMCID: PMC3753973. Briefly, genomic DNA extracted from human patient intestinal mucosa was subject to PCR, and reaction products were purified and normalized with SequalPrep (ThermoFisher).