GSM9793803: MACR_FAB_2; Homo sapiens; RNA-Seq
Cells were collected in RNA lysis buffer (350 μL/sample) and frozen at −80 °C until RNA isolation was performed. RNA was extracted from frozen samples using the RNeasy kit (Qiagen Germantown, MD) according to manufacturer's instructions and sent to Novogene Corporation Inc. (Sacramento, CA) for RNA sequencing. RNA quality was determined by bioanalyzer (Agilent Technologies, Santa Clara, CA) to have RIN (RNA integrity number) of over 9.0 for all samples. mRNA was enriched using oligo (dT) beads, followed by cDNA library generation using TruSeq RNA Library Prep Kit (Illumina, San Diego, CA).