RNA-seq of wasabi late stage replicate 2
RNA-seq libraries were constructed using the NEXTFLEX Rapid Directional RNA-Seq Kit 2.0 (PerkinElmer, Waltham, MA, USA) and sequenced on the Illumina NovaSeq 6000 platform (SEEDERS, Republic of Korea) to generate 150 bp paired-end reads. Raw sequencing reads were pre-processed using Trimmomatic to remove adapter sequences, low-quality bases, and reads containing more than 5% unknown bases (N).