Amplicon sequencing of Staphylococcus epidermidis isolated from nose
Metagenomic S. epidermidis population analysis from nose microbiotaIt is well established that the human nose microbiota contain S. epidermidis belonging to different genotypes. To characterize the individual composition and relative abundance of S. epidermidis lineages in nose microbiota of enrolled patients, a metagenomic approach was employed (PMID: 33243146) in 6 PJI patients, of which 4 overlapped with the SF ex vivo group (Table X). In total, 19 different STs were identified across the 6 patients, with a mean number of 9.5 STs (range 8 - 12) per patient. The population was dominated (>30% of all reads) by a single ST in 5/6 patients, in 2/6 patients a single ST represented more than 50 % of all reads (ST 278 and ST59 in patients 8 and 10, respectively) (Figure X). Novel STs or unclassifiable sequences according to the Epidome algorithm were identified in 6/6 patients. In 2/6 patients, metagenomic analysis identified the ST corresponding to the ST of the invasive S. epidermidis isolate in the nose microbiota (Figure X).