AvrRpm1-H2O-a2 (CRX1853397; CRA027799)
Total genomic DNA was extracted from root samples using the PowerSoil DNA Isolation Kit (Qiagen). The V3-V4 hypervariable regions of the bacterial 16S rRNA gene were amplified via PCR using the universal primers 341F (5'-ACTCCTACGGGAGGCAGCA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3'). The resulting PCR products were used for library construction. The final libraries were sequenced on an Illumina NovaSeq 6000 platform to generate 2x250 bp paired-end reads.