Pool-seq of Littorina saxatilis from Terence Bay
Individuals were first combined into mini-pools of five individuals, the gDNA of which was then mixed to create the final maxi-pools, targeting 70 individuals. The gDNA extraction procedure followed the protocol of Wilding et al. (2001), with modifications including the use of ethanol 100 instead of isopropanol, the implementation of two chloroform purification steps, and the omission of gel lock columns. The resulting pooled gDNA samples were prepared using the Illumina TruSeq DNA PCR-Free Kit. Libraries were sequenced at Macrogen on an Illumina NovaSeq 6000 system, generating 151 bp paired-end reads.