GSM9108098: HepG2_cells_CUT&Tag-H3K18Dla_0mM; Homo sapiens; OTHER
HepG2 cells treated with 0 or 20mM D-lactate for 48 hours were collected, quantified, and bound to Concanavalin A (ConA)-coated magnetic beads. The cells were resuspended in antibody buffer and incubated overnight at 4°C with a primary antibody targeting H3K18-Dla. After washing, secondary antibodies diluted in blocking buffer were applied at room temperature for 1 hour. Subsequently, Protein A/G-Tn5 transposase complexes were added and incubated with rotation for 1 hour to fragment and tag genomic DNA associated with the target protein. DNA library was constructed with TruePreP Index Kit V2 for Illumina (TD202, Vazyme Biotech). The library was purified by VAHTS DNA Clean Beads (N411, Vazyme Biotech)