GSM9761443: CTRL; Danio rerio; RNA-Seq
Melanoma and adjacent skin were dissected from zebrafish and enzymatically dissociated in Liberase TL (Roche) followed by mechanical dissociation, then filtered through 70 μm and 40 μm strainers. eGFP+ keratinocytes and tdTomato+ melanoma cells were isolated by FACS (BD FACSAria) and recombined at a 7:3 KC:melanoma ratio per condition. Cells were pelleted and resuspended in DPBS + 0.1% BSA. Library preparation was performed according to the manufacturer's instructions using the Chromium Single Cell 3′ Library and Gel Bead Kit v3 and Chromium Single Cell 3′ Chip G (10x Genomics). GEM generation and library preparation was performed according to kit instructions. Libraries were sequenced on a NovaSeq S4 flow cell.