GSM9543947: Cortex, NeuN pos. Nuclei, SPNT, 4; Phodopus sungorus; RNA-Seq
RNA was isolated by lysing samples in RLT buffer (RNeasy Mini Kit, Qiagen) supplemented with β-mercaptoethanol (1:100) and homogenised using QIAshredder columns (Qiagen), followed by additional homogenisation with a glass douncer. RNA was purified from the flow-through using silica membrane spin columns (RNeasy Mini Kit) according to the manufacturer's protocol. The RNA was eluted in 30 µl RNase-free H₂O and stored at −80 °C. RNA sequencing libraries were prepared using the TruSeq stranded mRNA Library Prep Kit (Illumina) according to the manufacturer's instructions.