GSM9104883: C2C12 myoblasts treated with 6 micromolar PFOS rep 2; Mus musculus; RNA-Seq
Cells were grown to 100% confluency in petri dishes and treated with 0, 2, or 6 micromolar PFOS for 24 hours. RNA was extracted with TRIzol (Life Technology, Carlsbad, CA USA) and chloroform. Quality was checked via Nanodrop. Library construction was performed by Novogene (Sacramento, CA USA) using messenger RNA purified from total RNA. Quality was checked with Qubit (Thermo Fisher Scientific, Walkham, MA USA).