GSM9688163: RNA-Seq, B16-F10, SSBP4 KO, Rep4; Mus musculus; RNA-Seq
Total RNA was extracted using an RNeasy mini kit (QIAGEN) with on-column DNase treatment according to manufacturer instructions. Total RNA was used to prepare cDNA libraries following manufacturer instructions of Illumina TruSeq Stranded mRNA kit (Cat. # 20020595). PolyA+ RNA was selected, fragmented, primed, and then transcribed to cDNA with reverse transcriptase. cDNAs were then converted to double-stranded cDNAs prior to end-repair, A-tailing, and adapter ligation.