RNA-Seq of top-grafted union bark: non-top-grafted citrus
(1) Enrichment of eukaryotic mRNA using magnetic beads with Oligo (dT); (2) Add Fragmentation Buffer to randomly interrupt mRNA; (3) Using mRNA as a template, synthesize the first and second cDNA strands, and purify the cDNA; (4) The purified double stranded cDNA was subjected to terminal repair, followed by the addition of an A tail and ligation of sequencing adapters, and then fragment size selection was performed using AMPure XP beads; (5) Finally, the cDNA library was obtained by PCR enrichment.