GSM8728319: CUTTag_IgG_HGC27-GA6; Homo sapiens; OTHER
Cell nuclei (1–2×10^6) were isolated using NE1 buffer (20 mM HEPES pH 7.5, 10 mM KCl, 0.5 mM spermidine, 0.1% Triton X-100, 20% glycerol) and coupled to ConA magpoly beads (Smart-lifesciences, SM04102). The DNA was then purified using VAHTS DNA clean beads (VAHTS, N411) and amplified with the TruePrep DNA Library Prep Kit V2 for Illumina (VAHTS, TD501). The pooled library was sequenced on a NovaSeq (Illumina) platform, employing 150 bp paired-end reads. Rabbit monoclonal anti-H3 acetylation at lysine 27 (H3K27ac) antibody (Abclonal, A7253) and anti-GLI3 antibody (Bethyl, A303-417A-1) were used as the primary antibodies. Goat anti-rabbit IgG (Novoprotein, N269) was employed as the secondary antibody.