WCS132
Total genomic DNA was extracted from rhizosphere soil samples using the E.Z.N.A. soil DNA kit (Omega Bio-tek).     DNA concentration and purity were assessed with NanoDrop2000 (Thermo Scientific) and quality checked by 1% agarose gel electrophoresis.  PCR amplification of the fungal ITS1 region was performed with barcoded primers ITS1F and ITS2R.   The purified products were quantified using Qubit 4.0 (Thermo Fisher Scientific).  Library construction was performed using the NEXTFLEX Rapid DNA-Seq Kit, involving adapter ligation, magnetic bead selection, PCR enrichment, and bead recovery.   Sequencing was done on the Illumina NextSeq 2000 platform.