GSM9213553: mRNAK3, Untreated, biol rep3; Tritrichomonas foetus; RNA-Seq
Total RNA from T. foetus parasites (strain K) was extracted using the RNAspin Mini RNA Isolation Kit (GE Healthcare Life Sciences). Three independent RNA samples were obtained from parasites incubated for 48 hours in serum-free culture medium (Treated). As a control, RNA was extracted from parasites cultured under standard conditions with serum suplementation (Untreated). To eliminate genomic DNA contamination, all RNA samples were treated with DNase I (Invitrogen) before Amplification RNA libraries for RNA-seq were prepared by al Quick Biology Inc. using TruSeq RNA Sample Prep Kit (Illumina).