LP18_SUPE: Superficial soil sample (SUP) collected from approximately one square meter of leaf litter and humus, and processed using flotation and Berlese extraction.
A 418 bp fragment from the 3' end of the mitochondrial COI barcode region (bc3') was amplified using primers Ill-B-F and Fol-degen-R with Illumina adapter tails. Three independent PCR reactions were performed per sample and pooled. Amplicons were purified using AMPure XP magnetic beads and used as template for a limited-cycle PCR to incorporate dual-index barcodes and Illumina sequencing adapters.