GSM8465619: NovaST_coronal_section_plate5; Gallus gallus; OTHER
Telencephalons from 15 days old domestic chickens were dissected and immediately snap-frozen in isopentane for 10 min. Afterwards, the telencephalon was embedded in Tissue-Tek OCT cryo embedding compound, 10μm sections were cut using a cryostat and placed on Nova-ST chips Pepsin was used to permeabilise the sectioned tissue and Maxima Rtase for the reverse transcription. Samples were treated with endonuclease and then tissue was cleared with Proteinase K. Klenow Fragment was used for second strand synthesis and the resulting cDNA was amplified and prepared for sequencing by addition of indexes and Illumina flow cell attachment sites.