aoc and lox10 mutant RNA-seq analysis of Fall army worm and Fusarium graminearum in the maize cultivar B104
aoc and lox10 mutant RNA-seq analysis of Fall army worm and Fusarium graminearum in the maize cultivar B104
Inner-whorl leaf tissue was harvested which included leaf tissue from 2-3 rolled leaves after two days post-fall army worm and control application. Temperature controlled greenhouse under normal light conditions Plants were infested by placing approximately 50 fall armyworm (Spodoptera frugiperda) into the whorl three-week-old plants.Â   RNA was extracted from 100 mg of ground tissue using the Zymo DIRECT-ZOL RNA Kit (Zymo Cat # R2051). rRNA was depleted using NEB depletion kit and libraries were constructed with NEBNext Ultra II directional library kit by the Iowa State University DNA Sequencing Facility.