C60213-genome-Hi-C-seq (CRX1774939; CRA026747)
Under the guidance of motor proteins, DNA/RNA chains bind to nanopore proteins embedded in the biological membrane and undergo denaturation. Driven by the voltage difference across the biological membrane, the DNA/RNA chains pass through the nanopore channel at a certain rate. Due to the differences in the chemical properties of the nucleotide bases, the passage of individual bases or DNA/RNA molecules through the nanopore channel induces distinct electrical signal changes. By detecting and correlating these signals, the types of corresponding bases can be determined, thereby enabling real-time sequencing.