eDNA metabarcoding of sediment and water samples: Bact3 (CRX1913297; CRA028973)
Total eDNA was extracted using the DNeasy Blood and Tissue Kit and the DNeasy PowerMax Soil Kit, a primer set Bact02 was used to amplify a fragment of the 16S rDNA, libraries were constructed using a PCR free protocol and sequenced on an Illumina NovaSeq 6000 platform with 250 bp paired end reads.