GSM8831716: E0771_3, Liver, 6°C; Mus musculus; RNA-Seq
Mouse tissues were homogenized in Trizol reagent (1 ml per 50 mg) and phase separation was performed by the addition of 0.2 ml chloroform per ml of Trizol reagent. The aequous phase was further processed using the Relia Prep RNA Tissue Miniprep reagent. The aequous phase was further processed using the Relia Prep RNA Tissue Miniprep System. Sequencing libraries from total RNA of the samples were prepared at the Core Facility Genomics, Medical University of Vienna, using the NEBNext UltraExpress rRNA depletion kits (NEB #E3330) for Illumina with Unique Dual Indices (UDIs) according to manufacturer's protocols (New England Biolabs