Genome skimming: Astragalus agnicidus - JB s.n.
Fragmentation with Bioruptor Pico sonicator using 5 cycles of 15 s On/90 s Off; NEBNext Ultra II DNA Library Prep Kit with no size selection and half volumes, 6 PCR cycles using NEBNext Dual Index Primers i706 and i505; Index Read 1: GAATTCGT, Index Read 2: CTTCGCCT