RNA-seq of Crisiella producta: Autozooid_A_rep3
Total RNA was extracted using TRIzol reagent. Poly(A) mRNA was enriched with the NEBNext Poly(A) mRNA Magnetic Isolation Module, and libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs). Sequencing was performed on an Illumina NovaSeq 6000 platform to generate 150 bp paired-end reads. Data cleaning included error correction with Rcorrector, quality trimming with BBDuk (Q<20), rRNA removal using SortMeRNA against the Silva database, and final adapter/poly-N trimming with TrimGalore