sequences of bacteria
The process begins with environmental samples, from which genetic material is extracted. Specific primers and adapters are designed and synthesized for targeted amplification. The extracted DNA undergoes PCR amplification, followed by purification of the PCR products to remove contaminants and enzymes. The purified products are then quantified and normalized to ensure consistent concentration across samples. Finally, the normalized DNA fragments are used to construct sequencing libraries, which are ready for downstream sequencing applications.