GSM9080389: Flat1_R1; Rattus norvegicus; RNA-Seq
Total RNA were extracted using the zymo extraction kit (R2060) Poly(A)+ mRNA was enriched from total RNA using oligo-dT beads, then fragmented and reverse transcribed using random hexamer priming. First- and second-strand cDNA was synthesized, followed by end repair, 5' phosphorylation, and dA-tailing. Indexed sequencing adapters were ligated, and the libraries were PCR-enriched and quality-checked before sequencing.