DNA-seq
Library preparation and sequencing was carried out by SeqCenter. Sample libraries were prepared using the PCR-free Oxford Nanopore Technologies (ONT)Ligation Sequencing Kit (SQK-NBD114.96) with the NEBNext Companion Module (E7180L) to manufacturers specifications. No additional DNA fragmentation or size selection was performed. Nanopore sequencing was performed on an ONT GridION or PromethION sequencer usingR10.4.1 flow cells in one or more multiplexed shared-flow-cell runs. Run design utilized the 400bpssequencing mode with a minimum read length of 200bp. Adaptive sampling was not enabled.