V3V4 16S amplicon seq of sus scrofa gut metagenome
V3-V4 region amplified with the primers 341F (CTTTCCCTACACGACGCTCTTCCGATC- CCTACGGGAGGCAGCAG) and 806R (GGAGTTCAGACGTGTGCTCTTCCGATCT- GGACTACNVGGGTWTCTAAT) using SuperHotTaq DNA polymerase (30 cycles, 65C annealing temperature). Single multiplexing performed using 6 bp index, added during a second PCR (12 cycles) with forward primer (AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGAC) and reverse primer (CAAGCAGAAGACGGCATACGAGAT-index-GTGACTGGAGTTCAGACGTGT). Purification with CleanNGS kit (Proteigene)