GSM8796744: Rank2, rep1; Mus musculus; OTHER
The colon contents were immediately removed and dispensed into sterile tubes. The colon contents from the upper segment were rapidly frozen in liquid nitrogen and stored at -80°C. Total microbial genomic DNA was extracted using the FastPure Stool DNA Isolation Kit (MJYH,shanghai, China) according tomanufacturer's instructions. The quality and concentration of DNA were determined by 1.0% agarose gel electrophoresis and a NanoDrop® ND-2000 spectrophotometer (Thermo Scientific Inc., USA) and kept at-80 ℃ priortofurther use. DNA was amplified by PCR. Purified amplicons were pooled in equimolar amounts and paired-end sequenced on an Illumina NextSeq 2000 PE300 platform (Illumina, San Diego,USA) according to the standard protocols by Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai, China).