GSM9831307: Gonadotropin pooled hashtagged scRNA-seq library; Mus musculus; RNA-Seq
Ovaries were dissociated into single-cell suspensions. Viable CD45+ immune cells were isolated by flow cytometric sorting. Individual samples were labeled with TotalSeq-B hashtag antibodies and pooled by treatment group prior to single-cell library preparation. Single-cell gene expression and TotalSeq-B hashtag oligonucleotide libraries were generated using the 10x Genomics Chromium Single Cell 3' platform according to the manufacturer's protocol. Libraries were sequenced on an Illumina HiSeq 2500.