Genome mapping of Nipponacmea schrenckii
PacBioCCS sequencing libraries were prepared using the SMRTbell Template Prep Kit v.1.0 (Pacific Biosciences Ref. No. 100-259-100). Prior to library preparation, the size spread of sheared DNA was labeled using a DNA12000 analyzer on an Agilent 2100 BioAnalyzer platform. After construction of 10 Kb small fragment libraries, sequencing was performed on a PacBioSequel II sequencer using the Sequel Sequencing Kit 3.0 chemistry