GSM8658898: Whole lung, LNP injected, rep2; Mus musculus; RNA-Seq
The rinsed tissue was added to the dissociation solvent (0.35% collagenase IV5,2 mg/ml papain,In 120 Units/ml DNase I) and incubated at 37℃ in a water bath shaker (100rpm) for 20min.After centrifugation, cell precipitates were collected and the cells were suspended with 100ul 1×PBS(0.04% BSA) solution.Red blood cells and dead cells are then removed. For scRNA-seq library preparation, cells were loaded onto the 10× Genomics Chromium instrument and 10× Genomics Chromium Single-Cell 3'kit V3 reagents were used according to the manufacturer's protocols. The samples were pooled into libraries and sequenced on the Illumina NovaSeq 6000 at a minimum depth of 20,000 reads per cell.