Gut microbiome of Penaeus monodon: gut
Gut DNA extracted, followed by quantification and 16s rRNA gene amplification using the Ion 16S Metagenomics Kit, library preparation using the Ion Plus Fragment Library Kit, followed by sequencing using the Ion Torrent S5 platform. Raw data (BAM) converted to FASTQ using SAM tool, quality check and trimming by FastQC and Trimmomatic software. OTU picking by QIIME software and diversity analysis (alpha, beta) and functional analysis using PICRUST.