GSM9864166: Library_04; Bos taurus; OTHER
Genomic DNA was extracted from whole-blood samples, and libraries were prepared with the NEBNext Enzymatic Methyl-seq Kit (New England Biolabs, Ipswich, MA) according to the manufacturer's instructions. This protocol utilizes TET2 oxidation and APOBEC deamination to achieve base-resolution methylation calls while minimizing DNA degradation Libraries were sequenced on the Illumina NovaSeq 6000 platform with 150 bp paired-end reads at the Génome Québec Innovation Centre (Montreal, QC, Canada). Each sample generated hundreds of millions of reads, providing deep coverage for CpG-resolution analysis.