GSM9242017: Blood, multiple samples, run 3; Mus musculus; OTHER
For each DO mouse, ~100 µl of blood was collected via tail nick 14 days before and 14 days after vaccination. RNA was extracted using the Mouse Ribopure Blood RNA kit (Ambion), treated with DNase to remove contaminating DNA, and purified using QiaQuick Spin kits (Qiagen). Libraries were prepared by Qiagen in 96-well format using the QIAseq UPX 3' Transcriptome kit. During reverse transcription, each sample is tagged with a unique ID, and each RNA molecule is tagged with a unique molecular index (UMI).