GSM9688018: E. coli rpoZ deletion; Escherichia coli K-12; RNA-Seq
Total RNA from 1 ml cell pellet was first extracted with RNAzol RT (Sigma), and the top water phase was further purified using Qiagen RNeasy Mini kit. The isolated RNA was treated with 15 units of DNase I (NEB, cat# M0303S) for 30 min at 37 ℃ to remove any remaining DNA. Samples were cleaned up by Monarch Spin RNA Cleanup kit (NEB). The rRNA was removed by using NEBNext rRNA Depletion Kit (Bacteria) (NEB) following the manufacturer's protocol. RNA library is prepared using Illumina Stranded mRNA Prep following the manufacturer's protocol.