sequence of fungi
Soil genomic DNA was extracted using the MP-Fast DNA Spin Kit for Soil. The fungal 18S rRNA gene was amplified using the specific primers SSU0817F (5'-TTAGCATGGAATAATRRAATAGGA-3') and 1196R (5'-TCTGGACCTGGTGAGTTTCC-3') with TransStart FastPfu DNA Polymerase. PCR products were purified using AMPure PB beads and quantified with the QuantiFluor-ST system. Sequencing libraries were generated using the TruSeq DNA Sample Prep Kit following the manufacturer's recommendations. The final library was sequenced on an Illumina MiSeq  platform.