GSM6705271: SKNO-1, KD; Homo sapiens; ATAC-seq
Nuclei were extracted from 50000 cells and tagmanted with 2.5 µl of TDE1 Enzyme (Illumina) for 30 minutes at 37C. Tagmented DNA was purfied with MinElute PCR Purification kit  (Qiagen). ATAC-seq libraries were amplified using 10-14 cycles on the thermocycler. Post amplification libraries were purfied with  Agencourt AMPure XP beads from Beckman Coulter. Libraries were validated using the Agilent High Sensitivity DNA Kit.