GSM8797523: B16-GFP Tumours (Day 8) biol rep 7; Mus musculus; RNA-Seq
Total RNA was extracted from whole tumours using TRIzol (Invitrogen, Waltham, USA) and purified using the RNEasy MinElute Kit (Qiagen, Hilden, Germany) according to manufacturers' instructions. Sequencing libraries were prepared using TruSeq Stranded mRNA Sample Prep Kit and 50 bp single-end read sequencing was performed using the Illumina HiSeq 2000 system (Illumina, San Diego, USA) according to manufacturer's instructions.