16SRNA:aquatic metagenome
For the study, water samples were collected from sites of annual isolation of Vibrio cholerae in the Zabaikalsky Krai and Irkutsk Region. Water samples from stationary sampling points for Vibrio cholerae on the Ushakovka, Angara, and Argun Rivers were analyzed. Samples were collected from the surface near the coast in the most stagnant and warmed areas in sterile 1-liter vials. The material was concentrated through a system of sterile polycarbonate filters with a pore diameter of 5, 1.2, and 0.22 m. Total DNA was then isolated. Microbial communities were characterized using Illumina MiSeq with amplification of the V3-V4 hypervariable region of 16S.