PEDV_screen_HeLa
Cells were infected with PEDV. Genomic DNA from both sorted cells and uninfected cells was extracted with Blood & Cell Culture DNA Maxi Kit (Qiagen).After genomic DNA extraction, sgRNA-containing region was PCR-amplified using Herculase II Fusion DNA Polymerase (Agilent). Resulting amplicons from the  PCR were gel extracted, quantified, mixed and sequenced using an Illumina NovaSeq 6000 platform.