GSM9141446: 3-months-OSK-rep1; Mus musculus; ATAC-seq
Cells were incubated with custom-loaded Tn5 transposase in high-salt transposition buffer for 30 minutes at 37 °C with agitation. Transposed DNA was purified using the Zymo DNA Clean & Concentrator kit. Libraries were pre-amplified for 5 cycles, then subjected to qPCR to determine the additional number of cycles needed, minimizing amplification bias. Final libraries were purified, quality-checked by gel electrophoresis, and quantified. Sequencing was performed on an Illumina platform using paired-end reads, targeting ~20 million reads per sample.