full-length 16S rRNA gene seq_C241 (CRX1592853; CRA024398)
DNA was isolated using the PowerSoil DNA Isolation Kit (QIAGEN, Germany) according to the manufacturer's protocol. The resulting PCR products were subjected to a series of processing steps, including damage repair, end repair, and adapter ligation, using the SMRTbell Template Prep Kit. The PCR amplicons were then purified using AMPure PB magnetic beads. Following quality control and quantification, the libraries underwent primer and polymerase attachment with the PacBio Binding Kit, followed by a final purification. The prepared library, meeting all quality specifications, was sequenced on the PacBio Sequel II system. submitted_instrument_model: Pacbio Sequel II