GSM9082629: iPSC-diff.1_PKR-eCLIP-seq_5nMAA_IN_Replicate2; Homo sapiens; RIP-Seq
For RNA-seq and dsRNA-seq experiments, RNA was extracted using Trizol and purified using Rneasy or ethanol precipitation. For eCLIP-seq and Riboseq experiments, RNA was purified using lysis buffer and gel-extracted. For RNA-seq experiments, librarires were prepared using the Illumina Stranded mRNA Prep (Ligation). For eCLIP-seq experiments, libraries were prepared using an in-house amplification procedure that closely follows the Illumina Stranded protocol.