GSM9110890: Tubuloids normal 3; Homo sapiens; RNA-Seq
Total RNA was extracted by initially homogenizing tubuloids in Sepasol-RNA I Super G (Nacalai Tesque, Japan), followed by purification using the Direct-zol RNA Miniprep Plus Kit (Zymo Research, R2070) according to the manufacturer's protocol.Given the limited amount of RNA, quality control was conducted using an Agilent TapeStation. Samples with DV500 values greater than 75% were confirmed by Relixa Inc. RNA-seq library preparation and sequencing were also outsourced to Relexa Inc. The library was prepared using the SMART-Seq mRNA HT LP (Takara Bio) kit. The sequencing was performed on an Illumina NovaSeq X Plus system, generating 2 × 150 bp paired-end reads (PE150), with approximately 6 giga bases of data and 40 million reads (20 million read pairs) per sample.