Control Mouse Liver Day 152
RNA-seq was performed using the Lexogen QuantSeq 3' mRNA-seq V2 kit (Lexogen Cat# 191.24) according to the manufacturer's instructions. 250 ng of total RNA was input into first-strand synthesis using oligo(dT) priming and subsequent RNA removal. Second-strand synthesis was performed using random priming followed by cDNA purification via SPRI-beads. Library amplification was performed using 12 nt UDIs over 17 PCR cycles and purified via SPRI-beads. Libraries were sequenced on a NextSeq 2000 using a 1x100bp strategy.