GSM8516329: iWAT_snATAC_Control; Mus musculus; ATAC-seq
Frozen iWAT (pooled from 10 mice per group) was homogenized, and nuclei were counted with the use of a LUNA-FL Automated Fluorescence Cell Counter. The nuclei were isolated with a Chromium Nuclei Isolation Kit (1000493, 10X Genomics) Tagmentation of accessible DNA regions with sequencing adaptors was performed with a 10X Genomics Chromium Instrument and ATAC kit (1000390 and 1000162, 10X Genomics). The quality of each library was determined with an Agilent Bioanalyzer, and the libraries were sequenced with a NovaSeq 6000 system (Illumina).