GSM8969447: ARID1A KO - ARID1A ChIP; Homo sapiens; ChIP-Seq
50,000 cells were washed once with 1 × PBS and resuspended in 100 μl lysis buffer (10mM Tris-HCl, pH 7.4, 10mM NaCl, 3mM MgCl2, 0.1% NP 40, 0.1% Tween-20, 0.01% Digitonin) for 3min on ice, followed by adding 1ml of wash buffer (10mM Tris-HCl, pH 7.4, 10mM NaCl, 3mM MgCl2, 0.1% Tween-20) to remove cytoplasm and mitochondria fraction. The nuclei were resuspended in 25 μl transposition mix (12.5 ul 2x TD buffer, 2.5 μl Tn5 Transposome, 8.25 μl 1xPBS, 0.25 μl 1% digitonin, 0.25 μl 10% Tween-20, 1.25 μl H2O) and incubated at 37 °C for 30 min in a thermomixer with 300 r.p.m. mixing. The tagmented DNA was purified using the DNA Clean & Concentrator-5 kit (ZYMO Research). Libraries were amplified and adapter dimers and primer dimers were cleaned up. Paired-end reads (50 bp) were sequenced using an Illumina HiSeq 4000.