GSM9212530: WT_RNA-seq_P1O_rep2; Mus musculus; RNA-Seq
Total RNA was extracted using the RNeasy Plus Micro Kit (QIAGEN, Cat # 74034) according to the manufacturer's instructions and the External RNA Controls Consortium (ERCC) RNA Spike-In Mix (Invitrogen, 4456740) was added to total RNA. Ribosomal RNA was depleted from total RNA using NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat) (NEB, E7400L). Library preparation was performed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB, E7760S) according to the manufacturer's instruction. Prepared RNA-seq libraries were sequenced on the NovaSeq X Plus system (Illumina) with paired-ended 150-bp reads.