AMPLICON of coastal bacteria day5
Filtered samples from cultured media were used for DNA extraction. The filters were processed using a PowerWater DNA Isolation Kit (MO BIO Laboratories, CA, USA) according to the manufacturer's instructions. The V3V4 region of the 16S rRNA gene was amplified using universal primers 341F and 805R. Amplicon sequencing was performed using the Illumina MiSeq platform (2300 bp).