Metatranscriptomic changes in propionate pathway and fiber degrading enzymes revealed the rebalancing mechanism of rumen microbiota after CH4 inhibition by 3-NOP in vitro
We chose a fully automated fermentation system in vitro to observe the process of 3-NOP inhibiting methane, and extracted the fermentation fluid (n=12) at the beginning of net hydrogen consumption(26 h) for metatranscriptomic sequencing, as well as quantitative analysis of key microorganisms.