DA79882 40 gen without TZP  Nanopore reads I-SceI digested pDNA
Plasmid DNA extracted using NucleoBond Xtra Midi (Macherey-Nagel); pDNA digested with I-SceI enzyme (Thermo Scientific) and purified using AMPure XP magnetic beads (Beckman Coulter) prior to library preparation. Sequencing library prepared using the ligation sequencing kit V14 with or without native barcoding kit 96 V14 kit; sequence performed on R10 cells