Illumina rna sequencing of As13
7 Adult aphids were homogenized with a mortar and pestle in TRIzol and extracted with chloroform and isoprenol and were then passed through Zymo RNA Clean & Concentrator kit (Zymo Genetics Inc., Seattle, WA, USA) to improve the purity and to remove gDNA using DNAse I. We then performed metatranscriptome Sequencing at Novogene (Novogene Corporation Inc., Sacramento, CA, USA). Library preparation was conducted using poly-A selection.