ChIP-seq experiment of AdpA binding to Streptomyces venezuelae chromosome
ChIP-seq experiment of AdpA binding to Streptomyces venezuelae chromosome
The liquid MYM medium supplemented with trace elements was inoculated to the final OD of 0.001 with the spores of S. venezuelae strains (Sven_WT – wild-type, control sample, and Sven_ΔadpA/adpA-FLAG – adpA deletion mutant complemented with adpA-FLAG under adpA native promoter) (Płachetka et al., 2021), and incubated for 12 and 20 hours at 30C with shaking (220 rpm). The cultures were prepared in two independent repetitions per strain using standard 250-ml flasks (flat bottom, w/o baffles) containing 50 ml of the medium.  A corresponding “input” DNA was prepared for each immunoprecipitation sample by taking a small fraction of the sonicated sample, followed by DNA precipitation. For all samples, the purified DNAs were subject to library preparation and Illumina sequencing at the Laboratory of Molecular Neurobiology Centre in Nencki Institute of Experimental Biology