liver_E2
Liver, kidney, heart, and skeletal muscle samples were taken from each animal. Containing microRNA RNA fractions were isolated from tissue samples using commercial miRNEasy kits (Qiagen, USA). Obtained samples were used to create cDNA libraries and subsequent sequencing. The quality of the isolated fractions was assessed using microelectrophoresis on Bioanalyzer chips (Agilent, USA). Samples with an RNA integrity index RIN of at least 8 were selected for sequencing. Sequencing was performed on the NextSeq platform (Illumina, USA) using NextSeq 500/550 High Output v2 kit (Illumina, USA). cDNA libraries from isolated RNA samples were prepared using NEBnext kits (NEB, USA), according to the manual. Qualitative and quantitative analysis of libraries was performed using Bioanalyzer microelectrophoresis (Agilent, USA) and Qubit fluorometry (ThermoFisher, USA). The quality of sequencing was assessed using the BaseSpace service (Illumina, USA) by the following parameters: cluster density, signal intensity in the detection channels, the proportion of clusters that passed the filter, by the output of aligned reads. All parameters were within the acceptable values.