Meiogyne hainanensis leaf transcriptome
mRNA are isolated from total RNA with oligo(dT) method. Then the mRNA are fragmented under certain conditions. Then first strand cDNA and second strand cDNA are synthesized. cDNA fragments are purified and resolved with EB buffer for end reparation and single nucleotide A (adenine) addition. After that, the cDNA fragment are connected with adapters. Those cDNA fragments with suitable size are selected for the PCR amplification. Agilent 2100 Bioanaylzer and ABI StepOnePlus Real-Time PCR System are used in quantification and qualification of those libraries.