GSM9169077: Xrcc4-/-::p53-/-; ESC-NPC; parental; DMSO; early; rep1; Mus musculus; OTHER
Genomic DNA for LAM-HTGTS: cells were lyzed in proteinase K digestion buffer, and genomic DNA were extraced with a standard phenol/chlorofomr protocol. RNA were removed by treating samples with RNAseA prior phenol/chlroform extraction. Genomic DNA for Repli-seq: cells were lyzed in proteinase K digestion buffer, fragmented by a Covaris fragmentation device to 200 bp, and nacent DNA were pulldowned by using an BrdU-specific antiboty. We follow the published protocol, as indicated below, to construct the libraries. Repli-seq: https://www.nature.com/articles/nprot.2017.148 LAM-HTGTS: https://www.nature.com/articles/nprot.2016.043