GSM8969514: Co-culture non-infected Monocytes,replicate3; Homo sapiens; RNA-Seq
Cells were then harvested by centrifugation at 1500rpm for 5 minutes and resuspended in lysis buffer RLT buffer with β-mercaptoethanol (Qiagen) for RNA extraction. Total RNA was extracted from human monocytes, synoviocytes or co-culture using the Qiagen RNeasy Mini Kit, following the manufacturer's protocol. Cells were lysed, homogenized, and RNA was purified using spin columns. 50ng of total RNA were processed using Illumina Stranded mRNA Prep Ligation kit (Illumina) to prepare cdna dual-indexed libraries according to manufacturer's recommendations