BNL CL.2  CRISPR knockdown screen MCD plus CuCl2 replicate 3
BNL CL.2 cells were transduced with the mouse GeCKO v2 pooled lentiviral CRISPR/Cas9 knockout library at MOI approximately 0.3, selected with puromycin for 3 days, and cultured in MCD medium supplemented with CuCl2 (10 M) for 2 weeks. Subsequently, genome sequencing was conducted, which constitutes biological repetition 3.