GSM9831037: NC2; Homo sapiens; RNA-Seq
RNA was extracted from stable cells and harvested using Trizol reagent. The suitable fragments are selected for the PCR amplification. During the QC steps, Agilent 2100 Bioanalyzer and ABI StepOnePlus Real-Time PCR System are used in quantification and qualification of the sample library. Then the library is sequenced using Illumina NovaSeq 6000. RNA libraries were prepared for sequencing using standard Illumina protocols