RNA-seq of Somniosus microcephalus: adult female liver
Total RNA was isolated from Greenland shark (Somniosus microcephalus) liver using a modified TriReagent protocol with high-salt precipitation. The lipid layer was removed after phase separation, and RNA integrity was evaluated by Bioanalyzer. The library was prepared using the Illumina TruSeq Total RNA Library Prep Kit with Ribo-Zero Plus rRNA depletion and sequenced on an Illumina NovaSeq 6000 platform, generating 101 bp paired-end reads.