NanoSeq of homo sapiens: normal human urothelium uninfected, FACS by low APOBEC3 and low BKPyV large T, undiluted control
Ureteric urothelial cells were isolated from excess tissue after renal transplant. Cells were expanded then underwent biomimetic re-differentiation, forming a tight barrier. Cells were maintained until 21 days to match the BKPyV infected cultures. Cells were then subjected to FACS using standard size, scatter and live/dead sorting followed by APOBEC3A/B/G and BKPyV Large T labelling. Cells were gated into four populations and DNA was harvested. DNA was then processed according to the Sanger Institute (Cambridge, UK) NanoSeq pipeline. Undiluted control.