Whole genome sequencing of bacteria:Bacillus velezensis
The SMRT bell library was constructed using the SMRT Bell TM Template kit (version 2.0) kit. The DNA samples qualified by electrophoresis were broken into target fragments of the required size with Covaris g-TUBE. After DNA damage repair and terminal repair, DNA adhesase was used to attach hairpin connectors to both ends of DNA fragments, and AMpure PB magnetic beads were used to purify DNA fragments, Blue Pippin fragments were used to screen fragments of specific size, and AMpure PB magnetic beads were used to screen SMRT Bell library for concentration, and then DNA damage was repaired. The SMRT Bell library was purified by AMpure PB magnetic beads again, and the constructed library was quantified by Qubit concentration, and the size of the inserted fragment was detected by Agilent 2100, and finally sequenced by PacBio platform.