GSM9081241: IL-2 Timecourse HTO library; Mus musculus; OTHER
Tregs were isolated from Foxp3IRES-GFP mice. Immunocytes were released from spleens using mechanical disruption followed by filtering and washes in phenol red-free DMEM containing 10mM HEPES (Gibco) and 2% fetal calf serum (FCS). Red blood cells in spleen samples were lysed before filtering using ACK lysis buffer (Gibco, ref A10492-01). Tregs were sorted as PI-Dump [CD19-CD8a-CD11c-NK1.1]-CD4+TCRb+GFP+ , and sent to the Broad Institute Genomics Platform for encapsulation and 10x Genomics protocols. Encapsulation was performed on the 10X Chromium instrument (10x Genomics). Libraries were prepared using Chromium Single Cell 3′reagents kit v2 according to manufacturer's protocol at the Broad