GSM9083004: B3, Infant, Day 3; Macaca mulatta; RNA-Seq
Following collection of peripheral blood (~0.5 mL per time point) in BD PAXgene tubes, the tubes were gently inverted 8-10 times and stored vertically at room temperature for a minimum of 2 hr. The tubes were then transferred to a −80 °C freezer where they were stored until the time of the RNA extraction. Total RNA was extracted using a Paxgene Blood miRNA kit (Qiagen cat# 763134), following the manufacturer's 775  instructions. The purified RNA was eluted in buffer BR5 (supplied in the kit), incubated at 65 °C for 5 minutes for RNA denaturation, and stored immediately at −80 °C. RNA samples were shipped on dry ice to Novogene Inc. for RNA quality control, library preparation and RNA sequencing. cDNA libraries were prepared at Novogene Inc. from total RNA using the NEBNext UltraTM II RNA Library Prep Kit for Illumina (NEB Cat# E7770L). Sequencing was performed on a Novaseq 6000 instrument (Illumina, Inc.).