Nanopore sequencing of male SHR rat genome
We pulverized liver tissue with a mortar and pestle in liquid nitrogen. We extracted high molecular weight DNA from the powdered tissue using the Monarch HMW DNA Extraction Kit for Tissue (New England Biolabs #T3060L), and prepared sequencing libraries with the Ultra-Long DNA Sequencing Kit V14 (Oxford Nanopore Technologies SQK-ULK114). We sequenced libraries on the Oxford Nanopore Technologies PromethION 2 Solo using R10.4.1 flow cells and used the Flow Cell Wash Kit (Oxford Nanopore Technologies EXP-WSH004-XL) to unblock pores and reload libraries twice during a 72-hour run.