RNAseq of Vicia faba:leaves
DNA libraries suitable for sequencing were prepared from 150 ng of total RNA with oligo-dT capture beads for poly-A-mRNA enrichment using the TruSeq Stranded mRNA Library Preparation Kit (Illumina, USA) according to manufacturers instructions ( volume). After 16 cycles of PCR amplification, the size distribution of the barcoded DNA libraries was estimated ~300 bp by electrophoresis on Agilent DNA 1000 Bioanalyzer microfluidic chips.