C60213-genome-ONT-seq (CRX1774937; CRA026747)
1) Cells were treated with the cross-linking agent formaldehyde to cross-link DNA with proteins, thereby fixing the conformation of DNA.   2) Cells were lysed, and high-quality DNA was extracted. The cross-linked DNA was then subjected to digestion with restriction enzymes.   3) End-repair was performed, and biotin was incorporated to label the oligonucleotide termini.   4) DNA ligase was used to ligate adjacent DNA fragments.   5) Protease digestion was conducted at the ligation sites to release the cross-linked proteins from the DNA.   6) DNA was purified and recovered, fragmented enzymatically, and labeled for capture. Sequencing adaptors were added to construct the sequencing library.   7) Paired-end sequencing (PE150) was performed using the Illumina NovaSeq6000 platform. submitted_instrument_model: OXFORD_NANOPORE PromethION