Smart-seq of Mus musculus
cDNA from NG2 glia was synthesized and amplified from ~100cells using SMART-Seq HT Kit User Manual (Takara Bio, USA) to generate double stranded cDNA for each replicate following the manufacturers instructions. The cDNA was subjected to SMRT sequencing at Shanghai Biotechnology Corporation (SHBIO, Shanghai, China) using PacBio RSII (Pacific Biosciences).