metatranscriptomic sequencing of Rn's gut in WH-Rn-06 (CRX1819655; CRA027291)
After removing host rRNA using the Ribo-off rRNA Depletion Kit (Human/Mouse/Rat) (Vazyme, China), the VAHTS Universal V8 RNA-seq Library Prep Kit for Illumina (Vazyme, China) was used for library preparation. Each RNA library was then sequenced on the Illumina HiSeq 2000/2500 platform (Genewiz Biotechnology Co., Ltd., China) with 155 bp paired-end reads .