GSM8999725: SPF mouse ileum, processed with 4 mM RVC, Sample 1; Mus musculus; RNA-Seq
The tissue was then dissolved in TRIzol™ (Thermo 15596026) and RNA extraction was performed by the Direct-zol RNA Miniprep Plus kit (Zymo R2071) Library preparation and sequencing was performed by the Harvard Medical School Biopolymers Core using the Ribo-Erase kit for rRNA depletion, the KAPA HyperPrep kit for library preparation, and 150-bp paired-end sequencing on an Illumina NextSeq 500.