P8.T1_GEX_R1 5' v2 gene expression
Following 10x Genomics protocol,  a maximum of 10,000 nuclei per sample were loaded into each channel of the Chromium Next GEM Chip K for processing on the 10x Chromium Controller (10x Genomics, Pleasanton, CA, USA). This was followed by cDNA generation and library construction according to the manufacturer's instructions (Chromium Next GEM Single Cell 5ʹ Reagent Kits v2 User Guide, Rev E). The resulting libraries were then normalized and pooled for sequencing on an Illumina NovaSeq system (Illumina, Inc., San Diego, CA, USA) with run parameters set to 26, 10, 10, 90.