GSM8864697: HCM3; Homo sapiens; RNA-Seq
Cardiac tissue was collected from the LV free wall in the operating room at time of transplant. The sample was kept in cold saline on ice during transfer to the laboratory for preservation. It was carefully dissected into multiple aliquots, which were flash-frozen and stored at –80 °C. Nuclear isolation was performed. Briefly, frozen cardiac tissue was dissociated by using a Dounce homogenizer. Single nuclei were isolated via fluorescence-activated cell sorting. SnRNA-seq was performed by using the 10X Genomics platform. Isolated nuclei were loaded into the 10X Genomics Chromium Controller to obtain the gel beads in emulsion. The sequencing libraries were then prepared according to the manufacturer's protocols for the Single-cell 3' Reagents Kits v3. Sequencing was performed by using the NovaSeq 6000 system.