Hi-C on Paracoccus aminophilus growing in exponential phase
Hi-C libraries were generated from bacterial cultures harvested at mid-exponential phase (OD600 = 0.3) after formaldehyde crosslinking (3%). Crosslinked cells were lysed by bead beating, and libraries were prepared using the Arima Genome-Wide Hi-C Kit following the manufacturers protocol. Libraries were purified with AMPure beads, sheared to ~500 bp with a Covaris S220, enriched on streptavidin beads using the Colibri kit (Thermo Fisher Scientific), and sequenced on an Illumina NextSeq platform (2 35 bp paired-end).