NextSeq 2000 sequencing
We performed bulk RNA sequencing on lymph node sorted lymphoma cells, disseminated either via lymphatic vessels or systematically through blood, collected respectively on day 11 and 18 post tumor injection, as well as on lymphoma cells taken directly from culture.
NEBNext Ultraexpress�RNA Library Prep Kit for Illumina (E3330S - New England BioLabs�Inc.) was used with the NEBNext�Multiplex Oligos for Illumina (New England BioLabs�Inc.) and NEBNext��Poly(A) mRNA Magnetic Isolation Module for cDNA synthesis with the addition of barcode sequences. The pre-pool sequencing was performed using the NextSeq2000 (Illumina, San Diego, CA, USA) with the P2 reagents kit V3 (100cycles; Illumina).�Samples were processed starting from stranded, single-ended 120bp-long sequencing reads.