HiFi sequencing of Sarcophoga bullata, adult male, thorax and head
HMW DNA was extracted from the head and thorax of a single adult male fly using the MagAttract HMW DNA kit (Qiagen), and sheared to a mean fragment length of 20 Kb using a Megaruptor 3 system (Diagenode). PacBio HiFi sequencing library was prepared following the SMRTbell Express Template Prep Kit 3.0 protocol (Pacific Biosciences). Sequencing was carried out on a PacBio Revio system using a 30-h movie collection time. Raw subreads converted into circular consensus sequencing (CCS) reads on-instrument using PacBio SMRT Link software v10.1 set to output HiFi reads.