GSM8970733: AAVS1-edited control NK cells from donor B77; Homo sapiens; RNA-Seq
RNA isolation from sorted NK cells were extracted using a RNeasy Plus Mini Kit (QIAGEN), according to the manufacturer's protocol. RNA samples were quantified using the Agilent 2200 TapeStation System (Agilent). Next-generation sequencing libraries were prepared with quality-controlled RNA samples (RIN > 7.0) from at least three biological replicates per genotype using an NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). cDNA libraries were pooled and subjected to 75 bp paired-end sequencing on a NextSeq 500 next-generation sequencer (Illumina) to obtain approximately 20 million reads per sample.