GSM9689268: Cells extracted from stiff matrix, stationary cells; Homo sapiens; RNA-Seq
Cells were extracted from hydrogels using the human tumor kit and the GentleMACs human tumor dissociator from Miltenyi Biotec (130-095-929). Cells were washed 3X with 1X PBS and stained with 7aad (Invitrogen A1310) according to the manufacturer's recommendations for dead cell identification. Cells were sorted using FACS to remove hydrogel particles and to ensure that only live cells were submitted for sequencing. Samples were prepared with 10X Chromium Single Cell 3ʹ v3 kit.