18S rRNA survey of IRL
Water samples filtered using mixed cellulose membrane filters. Choose primers to amplify near full length 18S rRNA. Library prep followed ONTs Four-Primer PCR Protocol, a distinct  ONT tag from the PCR Barcoding Kit (SQK-PBK004) was added to the 5 end of the primer  for each sampling location. Samples were loaded onto a prepared flowcell (FLO-MIN106D, R9.4.1) according to ONT protocols and sequenced