NanoSeq of homo sapiens: normal human urothelium exposed to BKPyV
Ureteric urothelial cells were isolated from excess tissue after renal transplant. Cells were expanded then underwent biomimetic re-differentiation, forming a tight barrier. Cells were then exposed to BKPyV and until half of the culture had succumbed to infection (LT50) when DNA was harvested. DNA was then processed according to the Sanger Institute (Cambridge, UK) NanoSeq pipeline.