GSM9111106: TBI5,IP; Mus musculus; OTHER
Total RNAs were extracted from injured cortical tissues using Trizol reagent following the manufacturer's protocol. Purified RNA was chemically fragmented into ~100nt segments and incubated with beads conjugated to anti_m6A antibodies. Eluted m6A_containing fragments (IP) and untreated input control fragments were converted into the final cDNA library by the dUTP method in accordance with strand_specific library preparation. Sequencing was performed on an Illumina NovaSeq 6000 platform with 100_base paired_end reads at LC_BIO Bio_tech Co., Ltd. (Hangzhou, China) following the vendor's recommended protocol.