GSM8580338: NK65-E_Day 5-2; Mus musculus; RNA-Seq
After samples thawed at 4°C, total RNA from purified cells was extracted using TRIzol (MiRNeasy Micro Kit, QIAGEN, #1071023) according to the manufacturer's recommendation. All samples had high-quality RNA (Agilent 2100 Bioanalyzer RNA Integrity Number (RIN) >7). The RNA sequencing library was prepared using the Illumina TruSeq PE Cluster Kit V3-cBot-HS (Illumina) following the provided protocol Sequencing was performed on the Illumina HiSeq platform (Illumina, San Diego, USA), generating standard 125 bp/150 bp paired-end reads.