GSM9112212: Gene expression library for CD11b+ enriched myeloid cells from mouse sarcomas, shSCR; Mus musculus; OTHER
Individual mouse tumors (n=5 each group) were digested with Miltenyi Mouse Tumor Dissociation Kit and gentleMACS running 37C_m_TDK_2 protocol. Cells were washed, strained, and red blood cells lysed. Cell suspensions were stained with Biolegend Total-Seq hashtags and fluorescent markers for FACS sorting, and washed 3 times. Cell suspensions were sorted to deplete tumor cells and enrich myeloid cells. Cells were pooled from multiple mice and captured on the 10x Genomics Chromium X controller. The 10x Genomics NextGEM 3' v3 Kit with Feature Barcoding was used according to manufacturer instructions.