GSM8580365: HL-7702 cells, KO, 3; Homo sapiens; RNA-Seq
Total cellular RNA was extracted using RNA-easy isolated reagent (Vazyme, R701) according to the manufacturer's instructions. RNA quantification was detected using NanoPhotometer (Thermo Fisher), followed by examination of RNA integrity and concentration with Agilent 2100 RNA Nano 6000 Assay Kit (Agilent Technologies). mRNAs were enriched by incubation with Oligo d(T) Magnetic Beads. Poly A enriched mRNA was fragmented and ﬁrst and second-strand cDNA was synthesized. The purified double-stranded cDNA was subjected to end repair, addition of base A, addition of sequencing junctions, and fragment screening to recover 350 bp cDNA. PCR enrichment was performed to obtain the cDNA library. After the constructed library was qualified by Qubit3.0 (Thermo Fisher) and Agilent 2100 bioanalyzer (Agilent Technologies), it was sequenced on the MGI DNBSEQ-T7 platform.