RNA-seq of 3t3-l1 treated with Taheebo 3
On day 9 of differentiation, total RNA was extracted from DMSO control- and Taheebo-treated (100 g/ml) cells, with each group consisting of three plates of independently differentiated cells. RNA extraction was performed using the RNeasy Mini Kit (Qiagen, Cat. No. 74104, Hilden, Germany) according to the manufacturers protocol. Complementary DNA (cDNA) libraries were generated from the extracted total RNA using the TruSeq Stranded mRNA Library Prep Kit (Illumina, Cat. No. 20020595, San Diego, CA, USA) following the manufacturers instructions. The libraries were sequenced on an Illumina NovaSeq X platform to produce paired-end reads (2  101 bp).