Hyb-Seq of Carex species
Genomic DNA was fragmented by sonication using a Bioruptor Pico Ultrasonicator. Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, MA, USA), quantified with a Qubit 2.0 Fluorometer, and pooled equimolarly. Target enrichment was performed using a Cyperaceae-specific bait kit following the myBaits protocol (Arbor Biosciences). Sequencing was carried out on an Illumina NovaSeq platform (150 bp paired-end reads).