GSM8797505: conditional knockout kidney-1; Mus musculus; RNA-Seq
Total RNA was extracted from wild type and conditional knockout frozen kidney tissue samples using Qiagen RNeasy plus Universal mini kit following manufacturer's instructions (Qiagen, Hilden, Germany). RNA sequencing libraries were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina, following the manufacturer's protocol (NEB, Ipswich, MA, USA) after enrichement for messenger RNAs using Oligo d(T) beads.