GSM9081805: Ri06, scRNAseq; Homo sapiens; RNA-Seq
A Chromium Next GEM Chip N (10X genomics) was loaded with the appropriate number of cells, and the sequencing libraries prepared with the Chromium Next GEM Single Cell 5' HT Reagent Kits v2 dual index following the manufacturer's recommendations. Briefly, an emulsion encapsulating single cells, reverse transcription reagents and cell barcoding oligonucleotides was generated. After the actual reverse transcription step, the emulsion was broken, and double stranded cDNA generated and amplified in a bulk reaction. This cDNA was fragmented, a P7 sequencing adaptor ligated, and a 5' gene expression library generated by PCR amplification.