GSM9082536: CHLA266_DAC_1; Homo sapiens; RNA-Seq
RNA extraction was carried out using the RNeasy Mini kit (Qiagen), including on-column DNase I digestion (Invitrogen). Integrity of genomic DNA was confirmed by gel electrophoresis. RNA was assessed on an Agilent 2100 Bioanalyzer using the Agilent RNA 6000 Nano kit. Strand specific mRNA libraries were generated by Novogene using the NEBNext Ultra II Directional RNA library prep Kit for Illumina (New England BioLabs #E7760), mRNA was isolated using Poly(A) mRNA magnetic isolation module (New England BioLabs #E7490). Stranded mRNA libraries were sequenced by Novogene on an Illumina NovaSeq6000 instrument,  using 150bp paired-end dual indexed reads.