Cucumis sativus root, 24mM NO3-, 4d LT, rep2 (CRX1828828; CRA027518)
Total RNA from cucumber root (100mg) with Trizol. PolyA mRNA enriched by oligo-dT beads. NEBNext Ultra II kit (E7770): fragmentation 94C 15min, cDNA synthesis, end repair, adapter ligation (Index1), size selection 300bp, USER digestion, 12-cycle PCR. Qubit/Bioanalyzer QC. NovaSeq 6000 PE150.