GSM8691816: WT blood, RRBS; Mus musculus; Bisulfite-Seq
DNA was extracted and adjusted to a concentration of ≥30 ng/µL, OD 260/280 ≥1.8 and OD260/230 ≥1.0. Methylation analysis of CpG regions, library preparation was performed according to the Zymo Research Zymo-Seq RRBS Library Kit (Cat No. D5460/5461) manual (Ver. 1.0.0), i.e., enrichment of CpG-rich regions by fragmentation of genomic DNA using the restriction enzyme Mspl, addition of the TruSeq adaptor, bisulfite treatment and addition of index sequences. The enriched DNA samples were amplified, and next-generation sequencing was performed.