GSM8691734: dHHO_WR_GN,PY61,Female,61 yr. [R1044]; Homo sapiens; RNA-Seq
PHHs, eHHOs and dHHOs were subjected to RNA extraction. The cells cultured in eHHOs minus OSM (eHHO_dOSM) and eHHOs minus OSM/WR (eHHO_dOSM_dWR) and dHHO minus Hormone-cocktail (dHHO_dHC) were also subjected to RNA extraction for comparison. Total RNA was extracted from organoids using a Direct-zol RNA Kit micro (Zymo Research #R2063) according to the manufacturer's instructions. 2 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using TruSeq RNA Library Prep Kit v2 (ILLUMINA) following manufacturer's protocols.