GSM9081948: wild type hESC, H2AK119ub, rep1; Homo sapiens; OTHER
Nuclei were extracted from cells and conjugated to ConA beads, which were then treated with Mnase. CUT&RUN libraries were prepared using NEBNext Ultra II DNA Library Prep kit for Illumina and NEBNext Mutiple Oligos for Illumina (Dual index). 5ng of DNA was used as starting material. Libraries were amplified using 14 cycles on the thermocycler. Post amplification libraires were purified with SPRIselect beads. Libraries were validated using Agilent High Sensitivity dsDNA kit.