GSM9081235: cxcr4a mutant; Danio rerio; RNA-Seq
10X GEX Single-Cell libraries are constructed using microfluidic technology to capture single cells and incorporate barcoded oligonucleotides during reverse transcription. The 10X Genomics scRNA-seq library strategy captures single cells in droplets, incorporates barcoded oligonucleotides during reverse transcription, and amplifies cDNA for library construction. The final library, compatible with Illumina sequencing, is sequenced using paired-end reads and analyzed with software like Cell Ranger for gene expression quantification.