GSM8797675: CD45+ cells, HCoV-OC43, WT; Mus musculus; RNA-Seq
Lungs from uninfected or HCoV-OC43-infected WT or Csf2rb-/- mice were collected and digested into single-cell suspensions, and CD45+ cells were sorted. Cell viability was assessed using Trypan Blue staining. Single-cell suspensions at a concentration of 2×10^5 cells/mL in PBS were loaded onto a microwell chip using the Singleron Matrix® Single Cell Processing System. Barcoding Beads were collected from the microwell chip, and mRNA captured by the Barcoding Beads was reversely transcribed to obtain cDNA, followed by PCR amplification. The amplified cDNA was then fragmented and ligated with sequencing adapters. scRNA-seq libraries were constructed using the GEXSCOPE® Single Cell RNA Library Kits (Singleron). Individual libraries were diluted to 4 nM, pooled and sequenced on an Illumina novaseq 6000 with 150 bp paired-end reads.