GSM9438631: ChIP-Seq, meiotic cells, Daxx knockdown, biol rep2; Mus musculus; ChIP-Seq
For H3K9me3 ChIP-seq, the spermatocytes and RS were sorted by FACS and were crosslinked with 1 % formaldehyde in DMEM for 10 min at room temperature. The ChIP experiments were performed with the ChIP-IT High Sensitivity Kit according to the manufacturers' instructions. Libraries were prepared using the VAHTS Universal Pro DNA Library Prep Kit for Illumina from Vazyme following the manufacturer's instructions. Libraries were sequenced a 150-bp paired-end mode on DNBSEQ-T7 platform.