Hic-SK1 (CRX1854190; CRA027830)
Treated with formaldehyde and other crosslinking agents, the crosslinked chromatin complex was released. DNA was cut with restriction enzymes (such as HindIII and MboI) to produce sticky ends. Biotin labeled nucleotides were used to fill the sticky ends produced by enzyme digestion and mark the breaking sites. The ligation reaction was carried out under the condition of low concentration of DNA. Reverse cross-linking, release DNA, purify and obtain the linked product. Biotin containing linker fragments were enriched by streptavidin magnetic beads. The DNA was broken to the length required for sequencing by ultrasound or enzyme digestion. The ngs library was constructed by PCR amplification. The Illumina platform was used for double ended sequencing. submitted_instrument_model: Illumina HiSeq X Ten