GSM8692834: Posterior blastema, 11 cm axolotl, 15 dpa, lower input, replicate 2; Ambystoma mexicanum; RNA-Seq
Total RNA was extracted using an in-house magnetic beads-based RNA purification kit (Vienna Biocenter Core Facilities) RNA libraries were prepared with QuantSeq 3' mRNA-Seq V2 REV kit with unique dual indices (Lexogen), using the following RNA inputs: higher input libraries (45 ng), lower input libraries (4.5 ng), transplantation libraries (4.5 ng), Hand2/mCherry libraries (0.7 ng). Samples were multiplexed for sequencing, using dual i5/i7 indices in the Quantseq kit.