Samples for HiFi library construction
15 g of high molecular weight genomic DNA was used to construct single molecule real-time (SMRT) libraries with an insert size of 15 kb, following the manufacturers standard protocol (Pacific Biosciences (PacBio), CA, USA). Libraries were sequenced on a PacBio Sequel II platform applying circular consensus sequencing (CCS) mode. Raw data were converted into HiFi reads employing the CCS and PBINDEX toolkit based on standard parameters