RNAseq ILB308 4dpi Piezodorus guildnii rep2
ILB308 was previously grown in MM. Fungal spores were collected from this culture media plates and spore suspensions were prepared for insect inoculation according to Sessa et al. (2022)14. P. guildinii uncopulated adult females were inoculated by immersion in a conidial suspension of 1x107 conidia/ml and maintained on an incubation chamber with 14/8hrs light/dark, at 25C with 70% humidity. At 4 days post inoculation (dpi) living P. guildinii individuals were removed from the incubation chamber and stored at -80 C until RNA extraction. RNA extraction was performed using the tissue, and the abdomen and thorax contents of dissected insects. Each sample (axenic cultures and inoculated insects) was ground with a RNAse-free mortar and pestle pre-cooled with liquid nitrogen, and total RNA was extracted using RNAeasy Plant isolation kit (Qiagen, Germany).