GSM8833420: E9.5-6mA IP; Mus musculus; OTHER
The process of 6mA MeDIP-Seq is briefly described as follows: Genomic DNA is extracted using the DNeasy kit (QIAGEN Inc.) and then sonicated to fragment sizes of 100-300bp. Subsequently, the fragmented DNA undergoes end repair, dA-tailing, and adapter ligation using the GenSeq® Universal DNA Library Prep Kit (GenSeq, Inc.). A portion of the adapter-ligated DNA, around 10%, is labeled as "Input" and kept aside, while the remainder is subjected to immunoprecipitation using the GenSeq® 6mA MeDIP kit (GenSeq Inc.). The DNA that underwent immunoprecipitation and the Input DNA are then subjected to library amplification using Illumina PCR primers and GenSeq® 2× HiFi PCR Mix (GenSeq Inc.). The purified DNA library is quantified using the Qubit fluorescence method (ThermoFisher) and sequenced on Illumina's NovaSeq sequencing platform. The DNA that underwent immunoprecipitation and the Input DNA that did not undergo immunoprecipitation were amplified into libraries using Illumina PCR primers and GenSeq® 2× HiFi PCR Mix (GenSeq Inc.).