DNA seq of untreated Ai9
Genomic DNA was extracted from the muscular tissue of injection site using a commercial extraction kit (Vazyme, DC102) following the manufacturers protocol. PCR primers (F: TCCTCCGGGCTGTAATTAGC; R: CCGAATTCGATCTAGCTTGG) were designed to amplify the region surrounding the target site. Then, 50 ng of Genomic DNA were used to perform PCR reaction using a 2  Phanta Max Master Mix (Vazyme, P525) with and an amplification thermocycling conditions: 35 cycles of 95 for 3 min, 95 for 15 s, 59 for 15 s, and 72 C for 60 s with a final extension time at 72 for 5 min. The PCR products were purified using a PCR Clean Up Kit (Beyotime, D0033) followed by the next-generation sequencing on sequel IIe platform of PacBio instrument (GENEWIZ).