Post-capture Nanopore sequencing of cucumber ds-cDNA library spiked with CGMMV, replicate 3
Barcoded double-stranded cDNA library generated from cucumber leaf RNA spiked with lyophilized positive control of cucumber green mottle mosaic virus (Tobamovirus viridimaculae) using template-switching reverse transcription and PCR amplification, followed by hybridization with 5'-biotinylated tobamovirus probes, streptavidin magnetic bead capture, and post-capture PCR amplification.