GSM9577983: spCHIP; Mus musculus; ChIP-Seq
Splenocytes were cross-linked with 1% formaldehyde, quenched with glycine, and lysed. Chromatin was sheared to 200-500 bp by sonication. Immunoprecipitation was performed overnight with anti-E47 antibody or IgG control coupled to Protein G beads. Beads were washed, cross-links were reversed at 65°C overnight, and DNA was purified using QIAquick PCR Purification Kit. DNA was purified using the QIAquick PCR Purification Kit. Libraries were constructed using the VAHTS TGS DNA Library Prep Kit  (Vazyme) following manufacturer's protocols.