NGS of genome sequence of Yarrowia lipolytica with plasmid HM12 after 384h mutation
The sequencing library was constructed by culturing Yarrowia lipolytica strains (carrying either control or hypermutation plasmids) in a 96-well plate. Three biological replicates were used, with eight technical replicates per biological replicate. The cultures were passaged every 48 hours, and genomic sequencing was performed on the mixed cultures every two passages.