Adult-RNA-rep1 (CRX913081)
The mRNA library was prepared using an optimized Smart-seq2 protocol. Briefly, 10 ng of total RNA for each sample was utilized for first-strand cDNA reverse transcription.The full-length cDNA was subsequently amplified through semi-suppressive PCR for 17 cycles. The amplified full-length DNA library was purified to get rid of short fragments and other contaminants using AMPure XP beads. 1 ng of purified DNA was exploited for library preparation using TruePrep DNA Library Prep Kit V2 for Illumina. Libraries were then quantified and sequenced using Illumina platform.