GSM8969240: 12-TAHSA (100 mg/kg; daily via oral gavage), LIDPAD, 4 weeks, replicate 3; Mus musculus; RNA-Seq
Intestinal mucosa of mice treated with different FAHFAs were harvested by scraping the luminal surface of the small intestine. Total RNA was extracted using the Pure-NA Fastspin Total RNA Extraction Kit (Everlife, Singapore). RNA sequencing libraries were prepared and sequenced on a DNBSEQ-T7 (BGI, China) with 150 bp paired-end reads.