GSM9865123: Donor 4 IL1R1+ memory Th17 cells_HTO; Homo sapiens; RNA-Seq
Following stimulation, cells were labeled with TotalSeq™ hashing antibodies (BioLegend) according to the manufacturer's instructions. For donors D3–D7, Media-, IL-1β-, and CD3-treated samples from each IL1R1 subset were pooled before 10x Chromium library preparation. For the additional donor, CD3- and CD3 + Th17 cytokine-treated samples were pooled using TotalSeq™ hashing antibodies, whereas the Day 0 sample was processed independently without hashing. Barcoded libraries were generated using the Chromium Next GEM Single Cell 3′ Reagent Kits v3.1 (10x Genomics, cat n° PN-1000123, PN-1000157, PN-1000122) according to the manufacturer's protocol. Libraries were pooled at a final concentration of 10μM and sequenced on an Illumina NovaSeq 6000 platform using 100-bp paired-end sequencing.