GSM8766308: Ripk1 EKO Sting EKO - Replicate 4; Mus musculus; RNA-Seq
Total RNA from FACS-sorted cells was extracted using RNeasy Micro kit (74004, Qiagen) with on-column DNase I digestion, according to the manufacturer's instructions. RNA integrity was tested using an Agilent RNA 6000 Pico kit (50671513; Agilent). Sequencing libraries were prepared using a Quantseq 3' mRNA ligation kit (Lexogen). Sequencing of the libraries was performed using an Aviti System (Element Biosciences).