GSM9830821: Ctrl_1; Homo sapiens; RNA-Seq
Clear cell renal cell carcinoma ACHN cells were divided into control group (Ctrl) and Curcumol treatment group. The treatment group was incubated with effective concentration of Curcumol, while the control group received vehicle treatment only. Multiple independent biological replicates were set for both groups under identical culture conditions. Total RNA was extracted from cell pellets for Illumina paired-end RNA-seq. After data quality control and transcript quantification, differentially expressed genes between two groups were screened, and GO, KEGG, GSEA enrichment analyses were conducted to uncover transcriptomic alterations triggered by Curcumol, further exploring the molecular mechanism of Curcumol modulating glycolysis and malignant phenotypes of ccRCC cells. PolyA mRNA was enriched from qualified total RNA and randomly fragmented. First-strand and second-strand cDNA were synthesized, followed by end repair, sequencing adapter ligation and PCR amplification to construct paired-end RNA-seq libraries. Eligible libraries were sequenced on Illumina high-throughput sequencing platform.