RNA-seq
RNA libraries were prepared with the MGIEasy RNA Library Prep Kit (MGI, China), and coding sequences were enriched using the NEXome Plus Panel v1.0 (Nanodigmbio, China). Library size distribution (~330 bp) and quality were verified using TapeStation DNA ScreenTape (Agilent, USA), and concentrations were measured via Qubit 2.0 (Invitrogen, USA) with the dsDNA High Sensitivity Assay Kit. Paired-end sequencing (150 bp) was performed on a DNBSEQ-G400 platform (MGI, China) using the DNBSEQ-G400 High-throughput Sequencing Set.