RNA-Seq of Synanceia horrida venom gland
Total RNA was isolated using TRIzol reagent (Invitrogen) according to the manufacturer's protocol. Subsequently, mRNA was isolated using Dynabeads (Thermo Fisher) according to the manufacturer's protocol. A total of 452.0ng mRNA (260/280=2.11) was obtained and submitted for sequencing. The mRNA was sequenced using a next-generation Illumina MiSeq Benchtop Sequencer at the Institute for Molecular Bioscience Sequencing Facility based on 2150 bp strand-specific paired-end runs