GSM9030601: ntTCF1SOX2delDBD,V5,hBJ,rep1; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a COVARIS. ChIP-seq libraries were prepared using the NEBNect Ultra II for DNA Library Prep, following manufacturers protocols, with an input of 5-10 ng of immunoprecipitated DNA. The  size distribution of amplified fragments was assessed by TapeStation, and the concentration of libraries was determined by Qubit.