GSM9865105: outer tepal from stage3 N.colorata floral bud repeat3; Nymphaea colorata; RNA-Seq
Total RNA was extracted from water lily floral bud tissues using TRIzol reagent. RNA concentration and purity were assessed with a Nanodrop 2000 spectrophotometer, integrity was evaluated by agarose gel electrophoresis, and the RIN value was determined using an Agilent 2100 Bioanalyzer. cDNA libraries were constructed using the Illumina TruSeq RNA Sample Preparation Kit, which includes poly(A) mRNA enrichment, fragmentation, reverse transcription, adapter ligation, and PCR amplification; the libraries were sequenced as 150‑bp paired‑end reads on an Illumina platform.