RNA-seq of the postero-dorsal head epidermis from high-nutrition: late prepupal male Onthophagus rectecornutus
Total RNA was extracted using the RNeasy Plus Micro Kit (Qiagen). Following quality control, poly(A)+ RNA was enriched using Oligo(dT) beads. Strand-specific sequencing libraries were constructed using dUTP-based second-strand marking and PCR amplification.