GSM8863815: HUDEP-2_PROcap_rep1; Homo sapiens; OTHER
Nuclei RNAs from run-on reactions with four biotin-labeled NTPs were extracted using the Total RNA Purification Kit (Norgen Biotek, 17200). Biotin-labeled newly transcribed RNA was captured on Dynabeads Streptavidin C1, followed by multiple on-bead processing steps, including adaptor ligation. The RNAs were then purified using TRIzol reagent. Reverse transcribed cDNA was amplified by UltraII Q5 polymerase and cleaned-up by SPRIselect beads and following Illumina Nextseq2000 sequencing.