GSM8970814: OCI-LY1, O/E empty vector, ATAC-seq, repeat 1; Homo sapiens; ATAC-seq
A total of 1×10^⁵ cells were washed with cold PBS and subsequently with ATAC-wash buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl₂, 0.1% Tween-20). Cells were then lysed using ATAC-lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl₂, 0.1% Tween-20, 0.01% IGEPAL CA-630, 0.1% Digitonin) for 10 minutes on ice. After lysis, cells were washed again with ATAC-wash buffer. The cells were spined down and the supernatant was thoroughly discarded. Tagmentation was performed using the Vazyme TruePrep DNA Library Prep Kit V2 for Illumina (TD501) at 37 °C for 30 minutes.The DNA was amplified for Illumina sequencing using NEB Next High-Fidelity 2× PCR Master Mix. The resulting library was purified via agarose gel electrophoresis, and fragments ranging from 200 bp to 700 bp were selected based on the DNA marker. The purified library was subjected to quality control using the Bioanalyzer.