ONT sequencing of Urtica dioica female
Nanopore sequencing libraries were generated from HMW DNA using the Genomic DNA by Ligation (SQK-LSK110) protocol. Sequencing was carried out with FLO-FLG001 R9.4.1 flow cells on a MinION with no base-calling. MinION generated fast5 files were subsequently basecalled using Guppy 6.0.1 Superior Basecalling Algorithm (dna_r9.4.1_450bps_sup.cfg) conducted on an NVIDIA 3060ti