GSM9177372: crCTR_4; Homo sapiens; RNA-Seq
Total RNA from MGRN1-KO (clone 4.9) and control cells (n = 4 for each condition) were isolated and purified with RNeasy® Mini kit (QIAGEN, Germantown, MD, USA) as per instructions. Novogene Co., Ltd. (Cambridge, UK) performed  quality control evaluations (all samples showed an RNA integrity number, RIN = 10), as well as cDNA synthesis, library construction (poly A enrichment), and transcriptomic analysis (Illumina Sequencing PE150, Illumina Inc., San Diego, CA, USA) yielding 15 G of raw data per sample.