16S rRNA gene (DNA) amplicon sequencing of peatland core subsample from: Natural-regeneration area|Spatial replicate B|Core replicate 1|Depth 6 cm
Microbial 16S rRNA genes were amplified using primers 515F and 806R targeting the V4 region. The PCR products with proper size were selected by 2% agarose gel electrophoresis. Same amount of PCR products from each sample was pooled, end-repaired, A-tailed and further ligated with Illumina adapters. Libraries were sequenced on a paired-end Illumina platform to generate 250bp paired-end raw reads.