GSM9762043: Young Cdh5CRE Brain endothelial cell RNA seq replicate 1; Mus musculus; RNA-Seq
Brains were rapidly isolated following euthanasia and mechanically dissociated to generate single-cell suspensions. Brain endothelial cells or endothelial nuclei were enriched using fluorescence-activated cell sorting (FACS) or endothelial marker-based purification methods. For scRNA-seq, viable single-cell suspensions were prepared immediately for library generation. For ATAC-seq and ChIP-seq, purified endothelial nuclei or chromatin were isolated according to standard protocols. For single-cell RNA sequencing, single-cell suspensions were processed using a droplet-based single-cell library preparation platform according to the manufacturer's protocol and sequenced on the Illumina NovaSeq 6000 platform.