GSM8552514: 6h Calorie restriction replicate 3; Mus musculus; RNA-Seq
RNA was isolated from 30 mg of female mouse liver tissue using mini spin column QIAGEN RNeasy Mini Kit. Non-directional – polyA enrichment library was prepared using NEBNext Ultra™ II RNA Library Prep Kit for Illumina (New England BioLabs), and 50M reads (150bp, paired end) per sample were generated on Illumina NovaSeq 6000 platform. RNA-seq reads were mapped to the mouse protein coding genes (Ensembl: Mus_musculus; GRCm38) using Bowtie allowing up to 2-mismatches. The gene expected read counts and Transcripts Per Million (TPM) were estimated by RSEM (v1.2.3). The TPMs were further normalized by EBSeq R package to correct potential batch effect.