GSM8970545: Ngru_30C_RNAseq_rep2; Naegleria gruberi; RNA-Seq
Fixed cell pellets are treated with 2 ul of 3%SDS, to achieve a final concentration of 0.5% SDS. Cells are thoroughly re-suspended and lysed on ice for 10 min. Lysates are transferred into 0.1 ml Bioruptor Microtubes (Diagenode C30010015, Liège, Belgium) and the chromatin was sheared using an NGS Bioruptor Sonicator (Diagenode) at High Intensity and cycles of 30'' ON/30'' OFF. RNA extracted with NEB Monarch RNA kit ChIP-seq / mRNA-seq