GSM9761938: control-1; Drosophila melanogaster; RNA-Seq
Total RNA for RNA-seq libraries was isolated from Canton S and sws1 mutant testes with the Quick-RNA MiniPrep kit. Ribosomal RNA was removed by hybridization of total RNA with biotinylated oligonucleotides complementary to different regions of rRNA followed by binding to Dynabeads™ MyOne™ Streptavidin C3 (Invitrogen by ThermoFisher Scientific, Waltham, MA, USA). RNA libraries were prepared utilizing the MGIEasy RNA Directional Library Prep Set (MGI, Shenzhen, China) according to the manufacturer's protocol. Sequencing was conducted on the DNB-SEQ-G400 platform (MGI, Shenzhen, China) in paired-end reading mode, with a read length of 150 bp.