GSM9028673: 4T1p; Mus musculus; RNA-Seq
GR-1⁺ cells were then isolated by positive selection using the EasySep Mouse PE Positive Selection Kit (BioLegend), according to the manufacturer's instructions. Purified GR-1⁺ cells were washed in PBS containing 0.04% BSA to minimize cell clumping and resuspended at a concentration of 1,000 cells/µL in PBS. RNA was immediately extracted. cells were processed using the 10x Genomics Chromium platform for single-cell sequencing. Library preparation was performed using the Chromium Single Cell 3′ Library & Gel Bead Kit v3 (10x Genomics).