Illumina paired-end genome sequencing of a virulent barcodedM. tuberculosis Erdman strain
An aliquot of the frozen barcoded Erdman library used for NHP infections (NR-50781, BEI resources) was plated on 7H10 plates at 37C. A single colony was grown in 7H9 broth with OADC supplement at 37C and bacterial genomic DNA extracted using phenol-chloroform extract6ion (see methods in Github) and sent for commercial sequencing using paired long read Nanopore and short-read Illumina sequencing (SeqCenter, Pittsburgh).