GSM8552742: GSC738, shCONT, replicate 1; Homo sapiens; RNA-Seq
Total cellular RNA was extracted from cell pellets using Trizol reagent and Direct-zol RNA Miniprep Kits Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads.After fragmentation, the first strand cDNA was synthesized using random hexamer primers,followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library. It was ready after end repair, A-tailing, adapter ligation, size selection,amplification, and purification.The clustering of the index-coded samples was performed according to the manufacturer's instructions.After cluster generation, the library preparations were sequenced on an Illumina platform and paired-end reads were generated.