GSM9438065: Monoclonal murine gastruloids – 10x lane 1; Mus musculus; RNA-Seq
Gastruloids were harvested and dissociated for single-cell profiling and processed following 10x Genomics standard protocols (Cell Preparation Guide CG00053 Rev C) Single-cell 3′ RNA-seq libraries were prepared using the 10x Genomics Chromium Next GEM Single Cell 3′ HT Reagent Kits v3.1 (User Guide Rev D). DNA Typewriter “DNA Tape” sequences were designed for recovery during scRNA-seq via inclusion of 10x Genomics Capture Sequence 1 (CS1). Libraries were sequenced on an Illumina NextSeq 2000.