GSM8635717: R3; Homo sapiens; ncRNA-Seq
Total RNA was extracted using the TRIzol reagent kit (Invitrogen) according to the manufacturer's instructions. RNA quality was evaluated with an Agilent 2100 Bioanalyzer and confirmed by RNase-free agarose gel electrophoresis. RNA libraries were prepared by removing rRNA to enrich for mRNA and ncRNA, followed by fragmentation, cDNA synthesis, and library preparation using the Illumina platform with adapter ligation, size selection, and PCR amplification according to the standard protocol.