GSM9793822: Bovine endometrium sample A_3, 17-OHPC/P4 formulation arm, day 7 treatment cycle; Bos taurus; RNA-Seq
Endometrial samples were collected using a sterile cytobrush introduced into the uterine horn ipsilateral to the corpus luteum and rotated once through 360 degrees. Collected material was immediately immersed in RNAlater and stored at -20 °C until RNA extraction and library preparation. Total RNA was extracted from each sample. mRNA library construction and RNA-seq were performed by Azenta Japan. One microgram of total RNA was used for library preparation. Poly(A)+ mRNA was isolated using oligo(dT) beads and fragmented. First-strand cDNA synthesis was performed using random primers, followed by second-strand cDNA synthesis, end repair, dA-tailing, adaptor ligation, size selection, PCR amplification, library validation, multiplexing, and 2 x 150 bp paired-end sequencing on a DNBSEQ-G400 platform.