GSM8516685: crypts isolated from Piezo double KO mice; Mus musculus; RNA-Seq
crypts were isolated from mouse small intestine using 20mM EDTA/PBS1X (Gibco) for 25min at 4°C with continuous shaking. Intestines pieces were then incubated in 1.5% sucrose/1% sorbitol/PBS1X solution and crypts were dissociated by vigorous shaking. Following 70um filtering and centrifugation, crypts were incubated with TrypLE 1X at 37°C for 5min. After pipetting multiple times until the single-cell suspension was attained, the solution is passed through a 40um strainer and centrifugated according to Manufacturer's guidelines : 10X genomics, single 3' v2 protocol