GSM8898499: PAX3FOXO1ΔHD_foxo1_input_rep1; Danio rerio; ChIP-Seq
Embryos were dissociated into single cells, fixed in 1% formaldehyde for 10 min, and quenched in 125 mM glycine. RH30 were cultured and fixed in a similar manner.  For the anti-FOXO1 ChIP-seq 2,000,000 zebrafish cells and 250,000 RH30 cells were sonicated with a bioruptor PICO and incubated with 4 μG anti-FOXO1 (Cell Signaling, C29H4). For the anti-H3K27ac ChIP-seq 1,000,000 zebrafish cells and1,000,000 RH30 cells were sonicated with a EpiShear probe sonicator and incubated with 4 μG anti-H3K27ac (Active Motif, 39133). DNA libraries underwent end-repair with either the End-It DNA End-Repair Kit (Lucigen, ER81050) or NEBNext End Repair (NEB, E6050S), A-tailing, and adapter ligation for Illumina sequencing. Libraries were selected with 1.8X AMPure beads selection, amplfied by qRT-PCR with Illumina sequencing adapters, and selected with a 1.0X AMPure beads selection.