GSM8699853: 007i SYN; Homo sapiens; RNA-Seq
Single cell suspensions were stained with Trypan Blue (Invitrogen, Waltham, CA) to determine cell suspension quality and cell viability.  Cell suspensions were partitioned (5000 cells target capture) into Gel Beads-in-emulsion (GEMs) on the Chromium Connect (10x Genomics, Pleasanton, CA) per the Chromium Automated Single Cell 3' Reagent Kits User Guide (v3.1).  Reverse transcription, cDNA amplification, and library construction were performed on the Chromium Connect. Barcoded libraries were quantified by quantitative PCR using the Collibri Library Quantification Kit (Thermo Fisher Scientific, Waltham, CA) and library size was measured via the 4200 TapeStation (Agilent Technologies, Santa Clara, CA).  Libraries were sequenced on a NovaSeq 6000 (Illumina, San Diego, CA) with sequencing configuration 28 x 10 x 10 x 90 bp, and a sequencing depth of 36,000 reads/cell.