Illumina NovaSeq 6000 paired end sequencing of SAMD01794765
The cDNA synthesis followed by library preparation steps for next-generation sequencing were performed by Smart-Seq Stranded Kit (TaKaRa Bio). The library preparation process incorporates double-stranded cDNA synthesis by random primers with the template switching technology, which maintains strand-orientation of the original RNA, and cleavage of ribosomal cDNA, which preserves both poly-A and non-poly-A mRNA-derived cDNA.