Pro-tumorigenic effects of HLA-G in Renal Cell Carcinoma
Pro-tumorigenic effects of HLA-G in Renal Cell Carcinoma
Tumor tissues derived from xenografts of RCC7/HLA-G1 and RCC7-WT cells were harvested 20 days post-implantation. Tumors were processed into single-cell suspensions using the gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec, Cat. No. 130-096-427) together with the Human Tumor Dissociation Kit (Miltenyi Biotec, Cat. No. 130-095-929), following the manufacturer's instructions. Total RNA was extracted using the RNeasy Mini Kit (Qiagen). RNA integrity was assessed using the Agilent 2100 Bioanalyzer, and only samples with RIN ≥ 8 were used for library preparation and sequencing. RNA libraries were prepared by the Integrated Genomics Core Shared Resources at Augusta University (RRID: SCR_026483). Libraries were constructed according to standard Illumina protocols for bulk RNA-seq.