GSM9111500: WTC11_Iso Control_S7; Homo sapiens; RNA-Seq
Total RNA was collected from hMGLs on day 25 of differentiation across all genotypes using the Pcipure RNA extraction kit (Life Technologies) mRNAseq was then performed upon these samples to identify transcriptional changes across all conditions using a NovaSeq 6000 sequencer at the Indiana University Center for Medical Genomics. RNA quality was determined with an Agilent Bioanalyzer 2011, with a RIN number of 7 or higher. cDNA library preparation included mRNA purification/enrichment, RNA fragmentation, cDNA synthesis, ligation of index adaptors, and amplification, following the KAPA mRNA Hyper Prep Kit Technical Data Sheet, KR1352 – v4.17 (Roche Corporation). Approximately 30M reads per library were generated.