CAP1_Ctrl_PostTAM_Matched_Lung_Rep3
Total RNA was extracted from tissue using TRIzol Reagent, and quality was assessed with a 5300 Bioanalyser and quantified using the ND-2000. Only high-quality RNA (OD260/280=1.82.2, RIN6.5, >1 g) was used for library construction at Shanghai Majorbio Bio-pharm Biotechnology Co. The RNA-seq library was prepared using Illumina Stranded mRNA Prep with 1 g of total RNA. mRNA was isolated via polyA selection and fragmented. Double-stranded cDNA was synthesized, followed by end-repair, phosphorylation, and 'A' base addition. Libraries were size-selected for 300 bp fragments and PCR amplified for 15 cycles. After quantification, paired-end sequencing was performed using the NovaSeq Xplus sequencer.