URMD-Seq of homo sapiens: fetal placenta
DNA was extracted from human placenta tissue and pre-labeled using primers that included a degenerate 12-bp unique molecular identifier (UMI) . UMI-tagged products were purified, quantified, and indexed using a second PCR. The resulting libraries were pooled and sequenced on an Illumina MiSeq platform with 2x300 bp paired-end reads.