GSM8694544: Q1, lung rabbit; Oryctolagus cuniculus; RNA-Seq
Rabbit lungs were extracted with the Monarch Total RNA Miniprep kit (New England Biolabs) by following manufacturer's instructions. From total RNA (300 ng/sample), selection of poly-A RNA was performed with the NEBNext Poly(A) mRNA magnetic isolation module (NEB) according to the manufacturer's protocol. Then, libraries were performed with the NEBNext Ultra II Directional RNA library prep kit (NEB) according to manufacturer's protocol. Libraries were paired-end sequenced (2 x 100 base pairs) on a NextSeq2000 device (Illumina), with a minimal read depth of 15 million reads.