Reticular fibroblasts counteract radiotherapy-induced head and neck tumor regression through Tenascin-C
Reticular fibroblasts counteract radiotherapy-induced head and neck tumor regression through Tenascin-C
Lymph node derived Fibroblast Reticular Cells (FRCs) were extracted from 10 week old male C57Bl6 mice either WT or TNCKO as previously described (Spenle et al., 2020, Cancer Immun Res, doi: 10.1158/2326-6066.CIR-20-0074). FRCs were plated for maximum 24 hours before 2 Gy irradiation, and then continued to culture for another 48 hours before collection. Total RNA was extracted using TriReagent (Life Technologies AM9738) Sequencing libraries were prepared using the Smarter Stranded Total RNA-Seq kit v3 Pico Input Mammalian following the manufacturer's instructions (Takara Bio, San Jose, CA, USA)