GSM9111609: 60day post TBI microglia Clodroante TBI  1; Mus musculus; RNA-Seq
FACSorted microglia were lysed and RNA extracted with the Arcturus PicoPure RNA isolation kit (ThermoFisher Scientific, Waltham, MA, USA), per manufacturer instructions. The cDNA was amplified through 11 cycles of PCR. Subsequently, cDNA libraries were prepared for Illumina sequencing using the Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA, USA). Sample quality control, processing, and library preparation were performed by the Northwestern University Next Generation Sequencing Core.