GSM8797515: scRNA-seq, iMGLs polyIC XP2, GEX; Homo sapiens; RNA-Seq
Total RNA was used for GEM generation and barcoding. Single-cell 3' v3.1 gel beads contain poly(dT) primers and additional primers (capture sequences). Followed manufacturer's protocol for Chromium Next GEM single cell 3' reagent kit v3.1 (dual index). cDNA amplification and cleanup followed by gene expression library construction and CRISPR screening library construction.