GSM8580405: V+3W_skin_mRNAseq_rep3; Vitis vinifera; RNA-Seq
TRIzol reagent (Ambion) Total RNA was extracted with TRIzol reagent (Ambion) from pericarps of fruits. For reverse transcription, 1 μg of RNA and oligo dT primers were used to synthesize cDNA in a 20-μL reaction using the qScript cDNA SuperMix kit (Quanta). Real-time PCR was then carried out on a CFX96 Real-Time PCR Detection System (Bio-Rad) with PerfeCTa SYBR Green FastMix (Quanta). For RNA-seq, the libraries were constructed and sequenced at the Genomics Core Facility of the Shanghai Centre for Plant Stress Biology, Chinese Academy of Sciences, with an Illumina HiSeq2500.