GSM9081125: RNA-seq of single blastomere (4cell_E01_B4); Bos taurus; RNA-Seq
Total RNA was extracted from single blastomeres using the SMART-Seq HT PLUS Kit (Takara Bio, Shiga, Japan), with modifications for single-cell analysis. Reverse transcription and cDNA amplification were performed using the SMART-Seq HT PLUS Kit (Takara Bio, Shiga, Japan). cDNA quality was evaluated using an Agilent 2200 TapeStation with High Sensitivity D5000 ScreenTape (Agilent Technologies). Libraries were sequenced on the NovaSeq 6000 platform (Illumina) with 150 bp paired-end reads.