RNA-seq of Somniosus microcephalus: adult female muscle
Total RNA was isolated from Greenland shark (Somniosus microcephalus) skeletal muscle using a modified TriReagent protocol with high-salt precipitation. RNA integrity was assessed by Bioanalyzer, and the library was prepared using the Illumina TruSeq Total RNA Library Prep Kit with Ribo-Zero Plus rRNA depletion. Sequencing was performed by DNA Link Co. (Seoul, Korea) on an Illumina NovaSeq 6000 platform, producing 101 bp paired-end reads.