Pool-seq of Littorina saxatilis from Holyrood Marina
Genomic DNA (gDNA) was extracted from intermediate mini-pools of five combined individuals. The mini-pools were combined into maxi-pools for sequencing to achieve 25 individuals per pool, following the general protocols described in Morales et al. (2019). The final pooled samples were prepared using the Illumina TruSeq PCR-free method, targeting 350bp fragments. The resulting libraries were sequenced at Psomagen USA on an Illumina sequencer setup for paired-end short-read data (150 bp) with a target coverage of approximately 100x.