ddRAD of Rhinella horribilis
DNA samples were cut using Msp1, and Sbfl-HF enzymes. Each sample was individually indexed and barcoded, with 48 individuals per library. Barcoded fragments were PCR amplified, and then size-selected to retain fragments between 200 and 700 bp. Final libraries were visualized on gels to ensure correct fragment size. Libraries were sequenced at the Cornell Biotechnology Research Center on the Illumina HI-SEQ 2500 with 100bp single-end sequencing with one lane per library.