GSM9107989: tumor01_CE467; Mus musculus; RNA-Seq
RNA from sorted cells was isolated using the PicoPure RNA Isolation Kit (Life Technologies, #KIT0204) according to the manufacturer's instructions. RNA-sequencing libraries were generated using the SMART-Seq mRNA library prep (Takara, #634768) and Unique Dual Index Kit (Takara, #634756) following manufacturer's protocol. Input included 1 ng of RNA and 1 ng of cDNA. Quality control was performed by assessing RNA, cDNA, and final library qualities using Tapestation. Libraries were sequenced in a NovaSeq 6k PE 150 S4.