WGS of ReMaSSing PE-2_QTL
genomic DNA was extracted using a modified protocol from Loke et al. (2011). DNA quantification was performed with a Qubit fluorometer, and quality was assessed by 0.9% agarose gel electrophoresis. After quality verification, at least 2g of each sample was dried in a SpeedVac and sent for sequencing. Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (New England BioLabs, Ipswich, MA, USA), and 2150bp paired-end sequencing (Q30>85%) was performed on the Illumina NovaSeq 6000 platform.