16S rRNA sequencing of E. caballus: Uterine fluid
The 16S amplicons were generated with the barcoded Full-Length Kinnex 16S primers from PacBio and the 2x Roche KAPA HiFi Hot Start Ready Mix. The amplicons were concatenated 12-fold with the PacBio Kinnex Concatenation Kit and Kinnex PCR 12-Fold Kit to produce 18kb libraries. The concatenated libraries were ran on the PacBio Revio sequencer with a 30hr movie time. CCS analysis, de-concatenation, and demultiplexing  were done using SMRTlink 13.1 using the following parameters: RQ>= 0.1044