GSM9764176: PBMC, DENV3, P4, biol rep 2; Homo sapiens; RNA-Seq
RNA was extracted with the Qiagen RNeasy Plus Mini kit RNA quality and quantity were assessed with the Agilent 2100 Bioanalyzer using the RNA 6000 Nano kit. Sequencing libraries were prepared with the Illumina Stranded Total RNA Ribo-Zero Plus library prep kit, using 20-50ng RNA as input. Libraries were assessed with the D1000 assay on the Agilent 4150 TapeStation and quantified with Qubit dsDNA High Sensitivity assay. A paired end 2x150bp sequencing run was performed on the Illumina NextSeq 2000 instrument.