NanoSeq of homo sapiens: normal human urothelium exposed to BKPyV then FACS by APOBEC3 and BKPyV large T
Ureteric urothelial cells were isolated from excess tissue after renal transplant. Cells were expanded then underwent biomimetic re-differentiation, forming a tight barrier. Cells were then exposed to BKPyV and maintained until 21 days post infection. Cells were then subjected to FACS using standard size, scatter and live/dead sorting followed by APOBEC3A/B/G and BKPyV Large T labelling. Cells were gated into four populations and DNA was harvested. DNA was then processed according to the Sanger Institute (Cambridge, UK) NanoSeq pipeline.