GSM9145401: Cachectic BAT, LLC injected 1; Mus musculus; RNA-Seq
Total RNAs from Brown Adipose Tissue were extracted using Direct-zolTM RNA MiniPrep (ZYMO RESEARCH) according to the manufacturer's instructions. Total RNA was quantified using the Qubit 4.0 fluorimetric Assay (Thermo Fisher Scientific). Libraries were prepared from 50 ng of total RNA using the NEGEDIA Digital mRNA-seq research grade sequencing service (Next Generation Diagnostic srl), which included library preparation, quality assessment and sequencing on a NovaSeq 6000 sequencing system using a single-end, 75 cycle strategy (Illumina Inc.).