GSM8694236: CAMA-1 Ribociclib-Resistant, Ribociclib + Afatinib, Day6 Hr6; Homo sapiens; RNA-Seq
Cells were collected for bulk RNA-seq on days 0, 6, 12, and 18 at 0, 6, and 24 h after media change. Cells were pooled, washed with PBS, and pellets were frozen at -80°C for subsequent RNA isolation. RNA was isolated using the Qiagen RNeasy® Plus Mini Kit (Cat. No: 74136) following the manufacturer's protocol. Library preparation was performed on the samples using the Illumina TruSeq Stranded mRNA Kit following the manufacturer's protocol.