DNA sequencing of fumarole microbiome
DNA was extracted using the DNeasy PowerSoil Pro Kit (QIAGEN) according to the manufacturer's instructions. The V4 variable region of the 16S rRNA gene was amplified using universal primers 515F and 806R. The ITS region was amplified using ITS3_KYO2 and ITS4. Amplicons were then purified using AMPure XP Beads. The Nextera XT Index Kits (Illumina, USA) were then used to index the amplicons.