HiC sequencing of Drosophila melanogaster strain A4 16-18 hours embryos Replicate 2
Hi-C libraries were prepared from 16-18 hour mixed-sex embryos using the Arima-HiC 2.0 kit (Arima Genomics), which utilizes a four-restriction enzyme cocktail. Proximally-ligated DNA was sheared, biotin-enriched, and libraries were constructed using the Accel-NGS 2S Plus DNA Library Kit (Swift Biosciences) following manufacturer protocols, then sequenced on an Illumina NovaSeq.