Illumina NovaSeq 6000 paired end sequencing of SAMD01936994
Total RNA was extracted from tomato (Solanum lycopersicum) roots inoculated with Fusarium oxysporum f. sp. lycopersici MAFF 103036. Poly(A) selection was performed using the NEBNext Poly(A) mRNA Magnetic Isolation Module, and strand-specific paired-end RNA-Seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit (dUTP method). In this library, Read 1 corresponds to the antisense strand and Read 2 corresponds to the sense strand of the original mRNA. Sequencing was performed at Rhelixa, yielding 150 bp paired-end reads. Raw (untrimmed) reads are submitted (23406854 read pairs).