Targeted NGS of Orientia tsutsugamushi traA and recA genes from bone marrow aspirate
Targeted next-generation sequencing (tNGS) using multiplex PCR primers targeting traA and recA genes of Orientia tsutsugamushi. DNA was extracted from bone marrow aspirate and amplified using primer sets GKS172P001 (traA) and GKS172P003 (recA).