Full-length 16S long-read sequencing of gut microbiome: Human feces
Library preparation was done with Express Template Preparation kit 2.0 with an initial input quantity of 1.7 g of amplified PCR products. The final Single Molecule Real Time (SMRT) library size obtained was 1903 bp. The binding kit used was Sequel II Binding kit 2.1, and 90 pM library was loaded onto the SMRT cell of PacBio Sequel II sequencing platform, containing 8M zero mode wave guides.