16S rRNA gene amplicon sequencing of Homo sapiens: fecal microbiota from rheumatoid arthritis patients receiving Anti-Inflammatory or Mediterranean Diet interventions
Stool samples from patients with rheumatoid arthritis enrolled in a randomized controlled dietary intervention trial were analyzed to characterize gut microbial community composition. Genomic DNA was extracted from fecal samples and the V3V4 region of the bacterial 16S rRNA gene was amplified using primers 515F and 806R. Amplicon libraries were prepared following the Illumina 16S Metagenomic Sequencing Library Preparation protocol and sequenced on an Illumina MiSeq platform using a 2  250 bp paired-end sequencing approach.