16-cell RNA-seq rep3
Embryos at the same developmental stage were collected manually under microscope. mRNA was extracted using the Dynabeads mRNA DIRECT Micro Kit (Life Technologies) following the manufacturers instructions. SMARTer Ultra Low RNA Kit for Illumina Sequencing (Clontech, USA) was used for cDNA synthesis and amplification. RNA-seq libraries were constructed using the VAHTS Universal DNA Library Prep Kit for Illumina V3 (Vazyme, China). RNA-seq libraries were sequenced using the Illumina platform.