HiC of Giardia duodenalis, strain P424
Hi-C libraries were prepared according to a previously published protocol (Grob S. Tough Tissue Hi-C. Methods Mol Biol. 2022;2532:35-50. doi:10.1007/978-1-0716-2497-5_3). Chromatin was digested using the restriction enzyme HindIII (New England Biolabs) and the fill-in reaction and marking of Hi-C fragments was conducted using biotin-dCTP. Illumina sequencing library preparation was performed using NEBNext Ultra II DNA Library Kit (New England Biolabs) by adapting the protocol slightly to allow library preparation on Streptavidin beads. Hi-C libraries were finally amplified with 15 cycles.