Whole Exome Sequencing of Bacterial Isolate
In nanopore sequencing, DNA or RNA is first extracted and prepared by adding sequencing adapters with motor proteins. These proteins guide the nucleic acid strand through nanopores embedded in a membrane. As each nucleotide passes through the nanopore, it causes characteristic changes in the ionic current, which are measured in real-time. These current disruptions are converted into raw signal data, which is then basecalled into nucleotide sequences. The long-read capability and real-time output make nanopore sequencing ideal for applications requiring quick, high-resolution insights.