Shotgun Metagenomic Seq CU_1
0.5g genomic DNA was randomly fragmented by Bioruptor Pico, and the interrupted DNA was filtered using magnetic beads. The DNA was repaired and adaptor was added, purification was performed using the magnetic beads. The products were amplified and enriched by PCR. Double-stranded PCR library is purified to unchain and then loop to form a single-stranded circular DNA (single stranded Circular DNA). The DNA nanosphere (DNA nanoball, DNB) was formed by rolling ring amplification (Rolling circle amplification, RCA) technology, and then the DNB was loaded into the chip and fixed through a fully automatic sample loading system.