GSM8797662: Cas9 LSK WT HSPCs transduced with Ncoa4 sgRNAs; Mus musculus; RNA-Seq
Lin-Sca+Kit+ cells were sorted using the Sony MA900 sorter based on the expression of BFP or RFP expression, which measures sgRNA transduction. 10000 cells were isolated per sample. Library was prepared using the single cell 5' reagent kit (10x Genomics) according to manufacturer's instructions. Library was sequenced on an Illumina Nvaseq SP.