16S Sequencing of mus musculus: intestinal regional contents
16S rRNA sequencing was performed at the University of Chicago Duchossois Family Institute in the MicrobiomeMetagenomics Facility (DFI MMF). DNA extraction from intestinal contents was performed using theQiaAmpPowerFecal Pro DNA (Qiagen, cat. # 51804) and the V4-V5 region of 16S rRNA- genes PCR amplified using barcoded dual-index primers (520FWD- AYTGGGYDTAAAGNG and 909REV-CCGTCAATTYHTTTRAGT). Illumina compatible libraries were generated using the Qiagen QIASeq 1-step amplicon kit (Qiagen, cat. #180419 and sequencing performed on an Illumina MiSeq platform using a 2250 Paired-End reads, generating >10,000 reads per sample.