GSM9829763: BxPC3,FOXA3 ko, Replicate3, RNA-seq; Homo sapiens; RNA-Seq
Total RNA was isolated using the Direct-zol RNA Purification Kit (Zymo R2062) with DNaseI treatment (n = 4 biological replicates). For the library preparation, 500ng total RNA was used for the RNA library preparation with Stranded mRNA Prep kit (Illumina 20040534) and RNA UD Indexes Set A Ligation kit (Illumina 20040553). Samples were sequenced on Illumina NovaSeq X sequencing platform to generate 50 million read pairs or more per sample.