BAT SCNx mouse 21 TRF ZT4
Single-nucleus RNA sequencing (snRNA-seq) was performed on interscapular brown adipose tissue (BAT) collected at Zeitgeber Time 16 (ZT16) on the second day of time-restricted feeding under subthermoneutral environment (TRF-STE). Each sample consists of BAT pooled from five Mus musculus (C57BL/6J) mice. The goal of this study is to investigate the impact of SCN disruption on BAT thermogenic adaptation under TRF-STE conditions. Samples were processed using the 10x Genomics Chromium Single Cell 3' v3.1 platform and sequenced on an Illumina NovaSeq 6000 system.