GSM9111404: five_prime_dex; Mus musculus; RNA-Seq
23 hours post treatment, mice were euthanized and BM cells collected. After ACK lysis, 8,000 mononuclear cells/sample were processed using the 10XGenomicsChromium single cell 3'V2 kit. For a subsequent repeat, we performed ACK lysis of samples from one mouse per treatment to obtain 5,000 mononuclear cells Library preparation was performed to the manufacturer's protocol (single cell 3'v2 and 5' protocol, 10x Genomics). Illumina libraries were sequenced on a partial Novaseq S4 lane to obatin 50,000 reads per cell.