NSUN2 shRNA knock down or control shRNA in 2D skin culture under differentiation conditions, using primary neonatal keratinocytes, to determine the function of NSUN2 in skin homeostasis.
NSUN2 shRNA knock down or control shRNA in 2D skin culture under differentiation conditions, using primary neonatal keratinocytes, to determine the function of NSUN2 in skin homeostasis. RNA was extracted for sequencing using an RNeasy Plus kit (QIAGEN, 74136). Libraries were prepared with QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen, 015.96).