RNA-seq profiling of wild-type C. elegans (N2) fed with succinate (N2_Sc) against untreated control and transgenic C. elegans expressing human α-synuclein (NL5901)
RNA-seq profiling of wild-type C. elegans (N2) fed with succinate (N2_Sc) against untreated control and transgenic C. elegans expressing human α-synuclein (NL5901)
Young adult worms were harvested and washed with M9 buffer and 0.2 % diethyl pyrocarbonate (DEPC) treated water, 3 times each, for removing any adherent bacteria and to inactivate RNase enzymes. QIAzol Lysis Reagent (1000μl) was added and worms were homogenized using pestle. RNA was isolated using QIAzol Lysis Reagent and miRNeasy Mini Kit. 500ng of total RNA was used for the construction of sequencing libraries. The Libraries were made using KAPA RNA HyperPrep Kit Illumina® Platforms following manufacturer's protocol. 