GSM9176795: Ppp1r15b(WT-1); Mus musculus; RNA-Seq
Total RNA was isolated from the cells using QIAzol Total RNA was isolated from the cells using QIAzol and then, RNA-seq analysis was conducted using OneStopRNAseq . Raw read quality control was performed with FastQC  and MultiQC, while post-alignment quality control was assessed using QoRTs. Paired-end reads were aligned to the mouse genome mm10 using star_2.7.5a and annotated with gencode.vM25.primary_assembly annotation. Aligned exon fragments with mapping quality higher than 20 were counted toward gene expression with featureCounts_2.0.0(91) with default settings except for the following parameters '-Q 20–minOverlap 1–fracOverlap 0 -p -B -C'. Differential expression (DE) analysis was carried out with DESeq2_1.28.1), and log2 fold change (LFC) shrinkage for each comparison was performed using 'ashr'. Significant differentially expressed genes (DEGs) were filtered based on the criteria FDR < 0.05 and absolute |LFC| > 0.585. Gene set enrichment analysis (GSEA) was performed using GSEA.