GSM9029120: TR1_500nM_LOXO292_2; Mus musculus; RNA-Seq
Total RNA was isolated using Quick-RNA MiniPrep Kit (Zymo Research), including the Dnase I treatment step Libraries were constructed using an Illumina TruSEQ stranded mRNA Sample Prep Kit (Cat# RS-122-2101). Total RNA was combined with RNA purification beads to bind PolyA RNA to oligodT magnetic beads. mRNA was eluted and converted to double stranded DNA. A-tailing, adapter ligation, and PCR amplification using 15 cycles was performed to complete the library construction.