bulk RNA seq data from radiotherapy-treated glioma-bearing mice
bulk RNA seq data from radiotherapy-treated glioma-bearing mice
After sacrificing the mice, brains were excised and tumors were selectively isolated. Tumor fractions were conserved in RNAlater for one week. Total RNA was extracted using TRIzol Reagent (Life Technologies, Thermo Fisher, cat n. 15596026) and RNeasy Mini Kit and RNase-Free DNase Set (Qiagen, cat n. 74104 and 79254). The concentration of RNA was quantified using a Qubit Fluorometer (Life Technologies, Carlsbad, CA) and RNA integrity was assayed using a TapeStation. Sequencing libraries were prepared with RNA with PolyA selection and ERCC spike-in with standard ERCC kit (ERCC RNA Spike-In Mix,Thermo Fisher 4456740).