A spatial map of human liver cirrhosis reveals the patho-architecture and gene signatures associated with cell state transitions during liver disease - single nuclei RNA-seq
A spatial map of human liver cirrhosis reveals the patho-architecture and gene signatures associated with cell state transitions during liver disease - single nuclei RNA-seq
Liver tissue was obtained following ethical approval and informed consent (National Research Ethics Service, REC14/NW1260/22) from the Manchester Foundation Trust Biobank. Fresh, unfixed tissue was obtained intraoperatively from patients undergoing surgical liver resections at the Manchester Royal Infirmary. Tissue was snap frozen within 30 minutes of collection and stored at -80ЛљC.  Used 10X sample collection protocol, detailed in CG000053_CellPrepGuide_RevC.pdfc Used 10X sample collection protocol, detailed in CG000053_CellPrepGuide_RevC.pdf