Pseudomonas phage GZ35,complete genome.
After the RNA sample is received, the sample is tested and then tested with a qualified oneSample library construction: First, the RNA sample is randomly interrupted and reverse transcribed and bistranded to synthesize double-stranded cDNAThe double-stranded cDNA is repaired to a blunt end by sticky ends, and then the base "A" is added to the 3' end so that the cDNA fragment can be ligated with a special adapter with a 3' base "T"; Finally, PCR is used to amplify the DNA fragments with linkers at both endsinto the construction of the entire library. Construct qualified libraries for cluster preparation and on-machine sequencing.