GSM9082295: D7_Naive_LN_3; Mus musculus; RNA-Seq
Tissues stored in TRIzol reagent were homogenized using a handheld microtissue homogenizer. After phase separation with chloroform, the aqueous phase was mixed with ethanol and total RNA was extracted using the RNeasy Mini Kit (Qiagen) per manufacturer's instructions. RNA quality and quantity were assessed using a Nanodrop One spectrophotometer and Agilent Bioanalyzer. Batches of RNA were shipped to LC Sciences (Houston, TX) for poly(A) RNA sequencing. cDNA library was prepared followed by Illumina TrueSeq-standard sample preparation protocol.