RNA-seq Pacbio MAS-seq 16-mer prepared with 10x 3' and 3dG TSO without TSO artifact cleanup of homo sapiens: PBMCs
The 3-Deoxyguanosine (3dG) template switch oligo was obtained from Biosyn Corporation, sequence: /5BiosG/AAGCAGTGGTATCAACGCAGAGTACATrGrG{3dG} with RNAse free HPLC purification. Following the standard 10x protocol, the 3dG TSO was resuspended to 1mM in elution buffer. The TSO comparison study was performed using PBMCs (Research Blood Components) on the 10x 3 Gene Expression Kit (10x Genomics, Cat #PN-1000269 ). The PBMC samples were run in parallel comparing following the standard protocol and a 3dG protocol with the only modification being the addition of 2.4ul of 1mM 3dG TSO in place of the standard TSO.