GSM9082309: ConA+SIM-3; Mus musculus; RNA-Seq
Mice were performed oral gavage with a 30 mg/kg body weight mixed solution of simvastatin or PBS per day before 5 days of ConA injection and 1 h before the injection. An additional oral gavage was administered to the mice 24 h after ConA injection.. CD4+ T cells in liver samples were stained and detected by fluorescence activated cell sorting (FACS) cells Aria II (BD Biosciences, CA, USA), CD3+ NK1.1-CD4+ CD8- cells were selected. RNA libraries were prepared for sequencing using standard Illumina protocols.