Co-treatment of Lignocellulosic Residues and Sulfate-rich Effluents for Value-added Molecule Production
DNA samples were obtained from the anaerobic sludge at the end of the experimental operation of diferent sequential batch reactors treating lignocellulosic biomass (sugarcane bagasse) and sulfate-rich effluents. Genomic DNA from the samples was extracted with FastDNA SPIN Kit for Soil DNA Extraction (MP Biomedicals), according to the manufacturer's recommendation. Oligonucleotide sequencing and basic bioinformatics service for taxonomic identification were performed by NGS Solues Genmicas (Piracicaba, SP, Brazil). The primer sets used in the amplification of the 16S rRNA gene were: 341F -785R for the Bacteria Domain (Klindworth et al., 2013) and Ar915aF  Ar1386R (Kittelmann et al., 2013) for the Archaea Domain. The data generated from sequencing by Illumina technology were analyzed using a set of packages implemented in the R language. The SILVA database was used as a reference