PacBio full length transcriptome sequencing
PacBio full-length transcriptome (IsoSeq) library: total RNA was reverse-transcribed to full-length cDNA using SMARTer PCR cDNA Synthesis Kit with oligodT priming; cDNA was PCR-amplified, end-repaired, ligated with SMRTbell adapters, and exonuclease-digested; sequenced on PacBio platform to generate CCS reads.