GSM8694515: 293T_3; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol™ reagent (Sigma-Aldrich) following the manufacturer's instructions. The total amount of 2 μg RNA was used as the starting material for RNA sample preparation. Sequencing libraries were constructed using the NEBNext Ultra™ RNA Library Preparation Kit (New England BioLabs, USA) according to the manufacturer's guidelines.