RNA-Seq of C. auris strain 20-1498 at 42C Rep2 R1
Transcriptomic analysis of Candidozyma auris strain 20-1498 under heat stress. The strain was initially cultured in YPD medium at 37C until reaching stationary phase. Cells were harvested and transferred to YNB medium supplemented with 2% dextrose and 0.5% (NH4)2SO4, then incubated for 6 hours at either 37C (control) or 42C (heat stress). Total RNA was extracted, polyA-selected libraries were prepared, and paired-end 150 bp sequencing was performed on an Illumina NovaSeq X Plus platform. Three biological replicates were analyzed for each condition to study differential gene expression under thermal stress.