DNA sequencing of HT-PAMDA library for SSM_20 enzyme on Plate3 at 1 min
HT-PAMDA was used to measure Cas9 PAM preferences by incubating Cas9gRNA complexes with a plasmid library encoding all possible PAMs adjacent to a fixed protospacer. PAM-specific cleavage rates were inferred from time-resolved depletion of sequences measured by Illumina amplicon sequencing.