Whole genome sequencing of Getah virus: Chinese isolates from swine
GETV complete genome amplification from pig serum samples using 12 overlapping RT-PCR fragments spanning the entire 11.7kb genome. Viral RNA was extracted using QIAamp Viral RNA Mini Kit, reverse transcribed with Superscript IV, and amplified with GETV-specific primers. PCR products were pooled in equimolar ratios, library prepared using Nextera XT DNA Library Prep Kit, and sequenced on Illumina MiSeq with 2300bp paired-end reads.