Post-capture Nanopore sequencing of pepper ds-cDNA library spiked with PMMoV, replicate 11
Barcoded double-stranded cDNA library generated from pepper leaf RNA spiked with lyophilized positive control of pepper mild mottle virus (Tobamovirus capsici) using template-switching reverse transcription and PCR amplification, followed by hybridization with 5'-biotinylated tobamovirus probes, streptavidin magnetic bead capture, and post-capture PCR amplification.