GSM8999996: Subject 31, paired blood sample, repeat 2, Day P+5; Homo sapiens; miRNA-Seq
Small RNA was isolated from 200–600 μL of plasma with miRNeasy Serum/Plasma Advanced Kit (QIAGEN, Hilden, Germany, Cat. No. 217204) following the manufacturer's protocol. For each sample, at least 10ng miRNA was used as input for the following library construction. The miRNA sequencing library was constructed using the QIAseq miRNA Library Kit (QIAGEN, Germany, Cat. No. 331502).