Flow based CRISPR screen lib seq of C42B(mNG2-AR) cell: 25% lowest mNG2-AR population
Genomic DNA was harvested with the QIAamp DNA FFPE Tissue Kit (cat# 56404). A standard genomic DNA PCR (22 cycles) was performed using NEB Next Ultra II Q5 master mix and primers containing TruSeq Indexes for NGS analysis. Sample libraries were prepared and sequenced on a HiSeq 4000.