GSM9176920: MA10 cells, Wildtype 2; Mus musculus; ATAC-seq
8-BrcAMP-treated cells were frozen in 10% DMSO containing medium. Nuclei are extracted from samples and tested for quality control (QC). After the QC, the nuclei pellets are resuspended in the Tn5 transposase reaction mix, which includes transposase and equimolar Adapter1 and Adapter 2. The mix of transposition reaction is incubated at 37°C for 30 min. PCR is then performed to amplify the library. Libraries were purified with the AMPure beads and library quality was assessed. After the construction of the library, the initial quantification was done with Tapestation 4150, and the library is diluted to 2-4ng/μl. Then the insertion size of the library is detected with NGS3K. If meeting the expectation, the accurate concentration of the library is quantified by Q-PCR (library effective concentration > 2nM) to ensure the accurate molar amount that will pooled for sequencing.