GSM9105209: GFB-15568; Mus musculus; RNA-Seq
For the RNA purification samples were first treated with DNase I (Thermo EN0521) to remove the DNA and purified using GeneJET RNA Purification Kit (Thermo K0732) according to the manufacturer's instructions. Purified RNA samples were quantified using Qubit high-sensitivity RNA Quantification assay (Thermo Q32852). The quality of the RNA was analysed using Agilent TapeStation systems with high-sensitivity RNA tapes with RIN numbers. FLASH-seq protocol (https://www.protocols.io/view/bulk-flash-seq-3byl4jynolo5/v2)