Adult-ATAC-rep1 (CRX913084)
50000 fresh cells were resuspended in 50 ul of ATAC-seq resuspension buffer and incubated on ice for 3 min.  Nuclei were resuspended in 50ul of transposition mix (10ul 5XTTBL (Vazyme TD501), 5ul TTE Mix V50, and 35ul water) and pipetted up and down 20 times to mix. Transposition reactions were incubated at 37  degreeCC for 30 min in a thermomixer. After the tagmentation, purify sample using the Ampure XP beads. The ATAC-seq library was amplified for 11 cycles of PCR with TAE mix (Vazyme TD501) following the manual. DNA was then sequenced using an Illumina sequencing platform.