Short read whole genome sequencing of Vibrio diabolicus: Isolate PHW6
Water samples were collected from a coastal region of South West England. Samples were concentrated using membrane filtration to determine the bacterial load using 0.45m filters that were then transferred onto Thiosulfate-Citrate-Bile Salts-Sucrose Agar (Oxoid CM0333) and incubated at 37C for 24 hours. Colonies were then counted (data not shown) and subcultured for purity on to Marine Agar (BD-DIFCOTM Marine Agar 2216). Bacterial DNA was extracted using the Maxwell extraction robot (Promega) by following instructions provided by the Maxwell RSC cultured cell DNA kit (Promega). The quality and quantity of extracted DNA was determined by Glowmax (Promega). Illumina sequencing was performed using Nextera XT libraries run on a NovaSeq 6000 sequencing instrument (2 x 250bp).