16S of tobacco-wheat rotation soil
The bacterial 16S rRNA gene V3-V4 hypervariable regions were amplified and sequenced. PCR amplification was performed using the primer pair 338F (5'-ACTCCTACGGGAGGCAGCAG-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3'). Sequencing was carried out on the NextSeq 2000 platform employing a paired-end (2x300 bp) strategy.