Targeted next-generation sequencing of Streptococcus pneumoniae: pediatric bronchoalveolar lavage fluid
Genomic DNA was extracted from pediatric bronchoalveolar lavage fluid. A targeted next-generation sequencing (TNGS) library was constructed by Eddiecare Medical Laboratory using streptococcus pneumoniae-specific probes for hybrid selection and enrichment, following standard Illumina library preparation protocols. The library was sequenced on an Illumina platform with paired-end reads.