GSM8489203: kidney, 10CBD, long-term,2; Mus musculus; RNA-Seq
Tissues were collected and immediately submerged in stayRNA™ (A&A Biotechnology) reagent. After 24 of cooling (4C), tissues were frozen at -80C until the time of RNA isolation. For RNA isolation, tissue scrapes (15-30 mg) were homogenized and used for RNA isolation with Trizol reagent (Thermo Fisher). Following isolation, RNA was controlled for quality with the TapeStation4150 system and quantified. Libraries were prepared using CORALL mRNA-Seq V2 (Lexogen) following standard protocol.