Parental4
RAW264.7 cells were plated on 6-well plates in complete media and harvested in cold PBS the next day. Total RNA was extracted using the RNeasy Kit (Qiagen), quantified by RiboGreen (ThermoFisher), and quality-checked on a 4200 TapeStation HS RNA ScreenTape (Agilent). Libraries were prepared from 500 ng total RNA with the TruSeq Stranded mRNA Kit (Illumina), with insert sizes verified on TapeStation D1000 and concentrations measured by PicoGreen (ThermoFisher). Paired-end 100 bp sequencing was performed on a NovaSeq 6000 (Illumina) at the St. Jude Hartwell Center.