GSM9144932: KMS12BM_Input; Homo sapiens; ChIP-Seq
Lysates were clarified from sonicated nuclei and isolated with anti-SETD7/9 antibody. The 75-nt sequence reads generated by Illumina sequencing (using NextSeq 500) are mapped to the genome using the BWA algorithm with default settings. Alignment information for each read is stored in the BAM format. Only reads that pass Illumina's purity filter, align with no more than 2 mismatches, and map uniquely to the genome are used in the subsequent analysis. In addition, unless stated otherwise, duplicate reads (“PCR duplicates”) are removed.