GSM8728256: hb34-1; Arabidopsis thaliana; RNA-Seq
Total RNA was extracted from 10-d-old seedlings using TRIzol reagent (Invitrogen, USA) and reverse-transcribed into cDNAs using commercial kits (TOYOBO, FSQ-301) following the manufacturer's instruction. Real-time PCR was performed using SYBR Green qPCR Master Mix (TRANS, AQ601) on the C1000 Touch™ Thermal Cycler (YEASEN). The constitutively reference genes were used as the endogenous control to normalize the amount of cDNA templates. RNA sequencing was carried out using the Illumina NovaSeq 6000 with Hiseq-PE150 strategy, conducted by Novogene Corporation (Beijing, China).