GSM8693359: P2116_4_GEX; Mus musculus; RNA-Seq
bone marrow cells were harvested and stained with CD4-bio, CD8-bio, Ter119-bio, CD11b-bio, Gr1-bio, B220-bio, c-kit-BV711, Sca-I-APC-Cy7, CD150-PE and CD48-AF700; and cell hashing CD45 antibodies (TotalSeq-B0301 to B0308), for sample barcoding (all from BioLegend) for 1 hour at 4°C, followed by 20-min incubation with streptavidin-PacificBlue. Cells were resuspended in staining media (PBS+ 3% FBS) containing Sytox Blue 1:5000, and HSCs (lin-, c-kit+, sca-1+, CD150+, CD48-), LSKs (lin-, c-kit+, sca-1+) and/or MyPs (lin-, c-kit+, sca-1-) were sorted using BD FacsARIA III (BD biosciences) with 85 um nozzle, into PBS + 3% FBS. Single Cell 3′ v3 Reagent Kit (10X Genomics) with BioLegend TotalSeq-B hashtagged antibodies (HTO)