RNA-seq Diatraea, fusarium
Total RNA was extracted from whole larvae using the RNeasy Mini Kit (Qiagen) following the manufacturers instructions. Genomic DNA was removed by DNase I treatment (2 U, 37 C, 20 min; Fermentas). RNA concentration and purity were assessed using a NanoDrop 2000 spectrophotometer (1.8  A260/280  2.0), and integrity was verified by agarose gel electrophoresis.