GSM9141334: CD8+ T cell, IgG, untreat, rep1; Mus musculus; ChIP-Seq
Cells were fixed with 1% formaldehyde for 8 minutes. After quenching and cell lysis, chromatin was sonicated using a Qsonica Q800R3 sonicator. ChIP-seq libraries were prepared using the VAHTS Universal Pro DNA Library Prep Kit for Illumina complemented with VAHTS Multiplex Oligos Set 4/5 for Illumina from Vazyme. 10 ng of DNA was used as starting material for per sample. Libraries were amplified using 13 cycles on the thermocycler. Post amplification libraries were size selected at 250-450bp in length using VAHTS DNA Clean Beads from Vazyme. Libraries were validated using the Agilent 4150 Tape Station System.