GSM9030621: iMGL-iNSpheroids, iPSC IMR90-4 cell line; Homo sapiens; RNA-Seq
The RNA sequencing workflow after RNA isolation included initial Poly-A selection-based mRNA enrichment, mRNA fragmentation, and random priming with subsequent first- and second-strand complementary cDNA synthesis. End-repair 5' phosphorylation and adenine nucleotide (dA)–tailing was performed. Adaptor ligation, polymerase chain reaction (PCR) enrichment, and Illumina NovaSeq technology–based sequencing with 2× 150–base pair (bp) read length were carried out.