sequences of * gene
After total RNA quality control, mRNA was enriched by rRNA removal (or by oligo(dT) magnetic bead enrichment for eukaryotic samples), fragmented, and reverse-transcribed into first- and second-strand cDNA using random hexamer primers. The double-stranded cDNA was then purified, end-repaired, A-tailed, adapter-ligated, size-selected, and PCR-amplified to construct the sequencing library, which was quality-checked before paired-end sequencing.