GSM9144040: HeLa, 1 ng/ml TcdB2 24 h, antibody isotype control (CUT&RUN), rep 1; Homo sapiens; OTHER
DNA sites binding for CTCF were isolated using CUT&RUN reagents obtained from Cell Signaling Technology (product # 86652).  Antibodies used for this assay include a rabbit monoclonal antibody against CTCF (Cell Signaling Technology; product # 3418); a rabbit monoclonal antibody against H3K4me3 (Cell Signaling Technology; product # 9751); and a rabbit monoclonal isotype control (Cell Signaling Technology; product # 66362). Sequencing libraries were constructed using XGen DNA Library Prep EZ Kit (IDT), and each library was indexed in order to multiplex for sequencing.