GSM9829714: P8 horiztonal cells; Mus musculus; RNA-Seq
Tg(Cx57icre;Ai14) mice were used to label and isolate horizontal cells. Retinas were dissociated using papain, followed by FACS sorting to isolate horizontal cells, then dissociated into a single-cell suspension for single cell capture and scRNA-seq library preparation Single cell libraries were generated using the Chromium 3' V3 platform (10X Genomics, Pleasanton, CA) according to the manufacturer's protocol. Briefly, single cells were partitioned into Gel-beads-in-EMulsion (GEMs) where nuclear lysis and barcoded reverse transcription of RNA would take place to yield full-length cDNA; this was followed by amplification, enzymatic fragmentation, and 5' adaptor and sample index attachment to yield the final libraries.