Genome sequencing of Bos frontalis: blood sample
Sequencing was performed on the Illumina HiSeq 2500 platform using a paired-end 150 bp (PE150) strategy, achieving a coverage depth of approximately 30X. During the process, DNA fragments were bound to specific primers and amplified via bridge PCR to form clusters. The sequencer then employed Sequencing By Synthesis (SBS) technology. Fluorescence-labeled dNTPs were sequentially incorporated based on the principle of complementary base pairing. The optical detection system simultaneously recorded the fluorescence signal intensity and the corresponding quality score for each cycle in real time.