CUT-Tag-Seq of ED cell (IgG)
The sequencing samples originated from DNA fragments labeled with the IgG antibody in ED cells.  Cells were harvested 48 hours post-transfection for protein retrieval. CUT&Tag experiments were conducted using the CUT&Tag kit (KP172-01, TransGen, China). A specific anti-Myc primary antibody was used to target the protein of interest. Protein A-Tn5 transposase complex loaded with sequencing adapters was then added to direct the transposase to cleave DNA at sites bound by the target protein, simultaneously inserting sequencing adapters at DNA ends. Sequencing libraries were generated by PCR amplification using the library preparation kit (KP101-11, TransGen, China).