Metagenomic sequencing of Tadarida brasiliensis: guano
RNA was extracted from guano homogenized in DNA/RNA Shield using the MagMAX Viral RNA Isolation Kit, followed by inhibitor removal and downstream processing with the RAPIDprep protocol for gDNA removal, rRNA depletion, cDNA synthesis, and cleanup (Tulloch et al. 2023). Double-stranded cDNA was used to prepare libraries with the NEBNext Ultra II FS DNA Library Prep Kit, bead-purified, quality-checked, quantified with a Qubit Fluorometer, and sequenced on an Illumina NextSeq 2000 (2  150 bp).