Genome analysis of novel Bradyrhizobium associated with South African indigenous legumes from the Grassland Biome
DNA samples were fragmented to an average size of ~500 bp using a Covaris S220 ultrasonicator. Sequencing libraries were prepared from 600 ng of fragmented DNA using the KAPA Hyper Prep Kit and uniquely indexed with the KAPA Unique Dual-Indexed Adapter Kit, followed by six cycles of PCR amplification. Size selection was performed with Kapa HyperPure Beads. Final library quality and quantity were assessed using an Agilent TapeStation 2200 and Kapa Library Quantification Kit qPCR, respectively.Paired-end sequencing (2 x 250 cycles) was performed on an Illumina NovaSeq 6000 platform using a NovaSeq 6000 SP Reagent Kit, spiked with 1% PhiX control.