Copy number abnormalities of chr1 in multiple myeloma at the single cell level
The DLP+ assay was done using a piezo droplet dispensing device, Cellenion's CellenOne F1.4. The assay utilized 72x72 uniquely indexed primer/adapter combinations which are spotted into a 5184-well Takara Smartchip utilizing two nozzles. Cells enumerated on a Countess II and stained using Live/Dead green dye as well as a proliferation stain. Cell spotting was performed on the CellenOne instrument using fluorescent markers to spot a single viable cell per well. Library preparation was performed on the CellenOne and tagmentation performed, remove Tn5 and finally adding PCR reagents. Thermal cycling was performed on a Bio-Rad T100 thermal cycler with a custom insert to house Takara Smartchips. Resulting libraries are recovered by spinning the Smartchips upside down into a collection vessel prior to library QC and sequencing. >1200 cells per sample were targeted. Sequencing was performed on Illumina's NovaSeq X, targeted 0.1x coverage per cell.