GENOMIC
Prepare 8 g of high-quality nucleic acid and use the BluePippin (Sage Science, Ipswich, USA) automated nucleic acid fragment recovery system to select the desired fragment sizes. For the selection process, prepare 1-1.5 g of high-quality nucleic acid, and use the NEBNext FFPE DNA Repair Mix (NEB, Ipswich, USA) and the NEBNext Ultra II End Repair/dA-Tailing Module (NEB, Ipswich, USA) to complete the damage repair and end repair with A-tailing of the nucleic acid fragments.Then, use the SQK-LSK109 kit (Nanopore Technologies, Oxford, UK) to ligate the sequencing adapters. Prepare the Flow Cell Priming Mix using the sequencing chip preparation kit (EXP-FLP001PRO.6, Nanopore Technologies, Oxford, UK). Configure the sequencing library using the SQK-LSK109 ligation kit. Run the PromethION Flow Cells (FLO-PRO002) on the PromethION 48 (Nanopore Technologies, Oxford, UK) sequencing instrument with the MinKnow software, and start the sequencing, running for 72 hours.