aoc and lox10 mutant RNA-seq analysis of Fall army worm and Fusarium graminearum in the maize cultivar B104
aoc and lox10 mutant RNA-seq analysis of Fall army worm and Fusarium graminearum in the maize cultivar B104
A 4-cm stem section centered on the stack node (includes immature stem, stack node, meristem, and immature leaves) was collected 2 days after inoculation. Temperature controlled greenhouse under normal light conditions  Four-week-old plants were inoculated by slitting the stems through the stack nodes and inoculating with 200 uL of Fusarium graminearum spore solution.  RNA was extracted from 100 mg of ground tissue using the Zymo DIRECT-ZOL RNA Kit (Zymo Cat # R2051). rRNA was depleted using NEB depletion kit and libraries were constructed with NEBNext Ultra II directional library kit by the Iowa State University DNA Sequencing Facility.