GSM9492248: Multi_ATAC_5FU_D8_LK; Mus musculus; ATAC-seq
Bone marrow was harvested by crushing bones in cold HBSS with 2% FBS (staining media, SM). All samples were filtered, treated with ACK red blood cell lysis buffer, magnetically enriched for cKit+ cells, and stained for surface protein expression. Live hematopoietic stem and progenitor cells were isolated on the basis of propridium iodide negativity and surface protein expression, either as lineage-/cKit+ cells (LK) or lineage-/cKit+/Sca-1+ cells (LSK). Libraries were constructed following manufacturer's instructions for Chromium Single Cell 3' Library & Bead Kit  and Multiome ATAC + Gene Expression Kit (10X Genomics). 5,000 cells were loaded for each sample and samples were sequenced in a NovaSeq 6000 sequencer (Illumina).