GSM8727803: shAB1; Homo sapiens; RNA-Seq
The RNA was harvested using TRIzol reagent (GK20008, GlpBio, California, USA). Total RNA sample was extracted and quantified with OD 260/280 ratio > 1.8 and OD 230/260 ratio > 2 were sent to BGI Genomics (Shenzhen, China) for sequencing. RNA libraries for RNA-seq were prepared using KAPA Stranded RNA-Seq Library Prep Kit (Roche) following manufacturer's protocols.