GSM8489663: EPSC in P2, 4C, Replicate 3; Homo sapiens; OTHER
Approximately 1 × 10^7 P2 hEPSCs were cross-linked with 2% formaldehyde for 10 minutes, quenched with 0.13 M glycine, lysed with lysis buffer (0.5% NP40 and 1% TX-100) The NEBNext® Ultra™ II RNA Library Prep Kit (NEB, E7770) was used to construct libraries for both the input and IP samples. 4C-seq primers carry overhang sequences such that after PCR the amplified products, but not the 3C template itself, are equipped with the up- and downstream (P5/P7) Illumina sequencing adapters and thus require no further library preparation for sequencing.