Standard-input RNA-seq of human B cells, Unstimulated, 0 hours, donor 10068703
Primary human B cells were isolated from healthy donors and stimulated in vitro. RNA was extracted using the AllPrep DNA/RNA Mini or Micro Kit (Qiagen) and submitted to the Center for Functional Cancer Epigenetics, Dana-Farber Cancer Institute, for standard poly-A enriched library preparation and high-throughput sequencing. See PMID 40475139 for additional details.