RNA-seq of Megaptera novaeangliae: male dorsal adipose tissue
Individual humpback whale adipose tissue transcriptome sequencing was performed using the Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus kit. 100 ng of RNA was mixed with the Hybridize Probe and rRNA Depletion Master Mix for library preparation. Paired-end sequencing was conducted at the Ramaciotti Centre for Genomics, University of New South Wales, Australia. Sequencing was performed on the NovaSeq X Plus System using a 2x100 bp run configuration and libraries were run on the 10B flow cell, including Illumina PhiX control library. The reads were demultiplexed in fastQ files and provided here.