GSM9177058: RS4;11 cells, HA-DNTTIP1_2, HA; Homo sapiens; OTHER
CUT&Tag assay was performed using the Hyperactive Universal CUT&Tag Assay Kit for Illumina (Vazyme, TD904, China). Cells were bound to ConA magnetic beads (10 min, RT) and incubated overnight at 4°C with primary antibodies (anti-HA/DNTTIP1). The next day, samples were sequentially incubated with secondary antibody (1 h, RT) and pA-Tn5 transposase complex (1 h, RT). After extensive washing, DNA was fragmented and eluted. Libraries were prepared using the TruePrep Index Kit V2 for Illumina (Vazyme, TD202) and sequenced on the Illumina NovaSeq 6000 platform (paired-end, 150 bp).