GSM9437250: Control_1; Danio rerio; RNA-Seq
Whole larvae (n=20) at 5-dpf were collected in RNA later (Life Technologies, AM7020) and stored at -80⁰C until use. Total RNA was extracted using the HiPure Total RNA Mini Kit (GeneBioSystems, Inc., Cat. No. R401103), following the manufacturer's protocol. Concentrations of total RNA were measured spectrophotometrically using a NanodropTM instrument (Thermo Scientific, Waltham, MA, USA). RNA integrity was assessed using 4200 TapeStation System (Agilent, CA, USA). Samples with an RNA integrity number (RIN) > 8 were sent to Genome Quebec Innovation Centre (MacGill University, Montreal, QC, Canada) for 200 cycles paired end (2 x 100 bp) sequencing on an Illumina NovaSeq PE100 - 25M reads (±5M reads) (Illumina Inc, San Diego, CA, USA).