GSM8516355: ATAC_mit__dEP400IR_NSC rep2; Drosophila melanogaster; ATAC-seq
For ATAC-seq carried out by using mitotic neural stem cells, the cells were fixed with 1% formaldehyde for 7min and quenched by Glycine. The cells were lysised and tagmented by using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme). ATAC-seq libraries were prepared using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme) complemented with TruePrep Index Kit V2 for Illumina (Vazyme). About 5k cells were used as starting materials. Libraries were amplified using 12-15 cycles on the thermocycler. Post-amplification libraries were size selected at 50-1000 bp in length using VAHTS DNA Clean Beads (Vazyme).