GSM8581821: Tfh, HC_A5, scRNAseq; Homo sapiens; RNA-Seq
To characterize the Tfh cells, CD4+ T cells were enriched from the peripheral blood mononuclear cell (PBMC) by positive selection using CD4 microbeads (Miltenyi Biotec). Enriched CD4+ T cells were then stained with Abs against CD4, CD3, CD25, CXCR5, and PD-1, and then sorted for Tfh cells, defining as CD4+CD3+CD25−CXCR5+PD-1+. After sorting, cells were suspended in DPBS. Single cells were separated into droplet emulsions according to manufacturer's instructions (10x Genomics). scRNA-seq was performed according to manufacturer's instructions (10x Genomics). 10x Genomics library construction kit (Cat # 1000190)