GSM8581887: WT-UI_L004; Danio rerio; RNA-Seq
Uninjured control hearts and 3 days post amputation (dpa) were extracted, washed in 1x PBS and and flash frozen in liquid Nitrogen. Nuclei were isolated as described in methods section using the Chromium Nuclei Isolation Kit with RNase Inhibitor, PN-1000494. Library was constructed using the Chromium Next GEM Single Cell 3' Reagent Kits v3.1(Dual Index) according to the manufacturer's protocol and using the 10X Chromium Controller.