GSM9791357: Epidermis, Naked mole-rats, Controls DMBA/TPA; Heterocephalus glaber; RNA-Seq
For RNA-Seq analysis of epidermis, skin was collected and cleaned thoroughly to remove any fat or muscle tissue and washed in PBS. Skin was then digested overnight in Dispase II (5mg/ml) in HBSS in +40C. After overnight digestion, epidermis was carefully separated from the dermis, homogenized in TRI Reagent and RNA was isolated using Direct-Zol Miniprep Kit following manufacturer's protocol. Libraries were prepared using Next Generation Sequencing Library Preparation kit from NEBNext® according to manufacturer protocol. Ribosomal RNAs (rRNAs) were depleted using NEBNext® rRNA Depletion Kit v2 and libraries were prepared using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®. After purifications, quality of the libraries was assessed on 5200 Fragment Analyzer with DNA Chip. For RNA-sequencing library preparation, concentration, quality and integrity of RNA was checked, and RNA with RIN>7 was used for the libraries.