NGS analyses of white truffle prokaryotic communities
Extraction of total genomic DNA from T. magnatum ascomata with a commercial kit; 16S rDNA gene amplification (V3-V4 regions) with the primer pair Pro341F (5-CCTACGGGNBGCASCAG-3) and Pro805R (5-GACTACNVGGGTATCTAATCC-3), modified with universal tails [Takahashi et al., 2014].