Genome sequencing of Aurantibacter sp. UH7, Nanopore reads
Whole genome amplification was performed from 1 microliter of an early exponential culture using the REPLI-g Single Cell Kit (Qiagen). The amplification reaction was performed in quarter reactions. During the first DNA purification step, all volumes were downscaled accordingly. Materials und tools used: ONT Sequencing Kit: SQK-NBD114.24, ONT Flow cell: FLO-MIN114, Basecaller: Dorado 1.0.0, Basecalling_model: dna_r10.4.1_e8.2_400bps_sup@v5.2.0, Demultiplexing: Dorado 1.0.0, Q10 filtering: Dorado 1.0.0, Read QC long reads: NanoPlot 1.44.1, Assembly: Flye 2.9.6 (--nano-hq), Long-read Polishing: Medaka 1.7.2, Medaka Model: r1041_e82_400bps_sup_g615