GSM9178129: Het_Pos465; Mus musculus; RNA-Seq
RNA was isolated from frontal cortex using the Qiagen RNeasy mini kit. Samples were further processed with the Zymo Research RNA clean and concentrate kit. After poly-A selection, RNA was fragmented and subjected to first strand cDNA synthesis using random hexamers. Second strand DNA was synthesized using dUTPs for the directional library and dTTPs for the non-directional library. The directional library was then prepared by end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification. The non-directional library was prepared by end repair, A-tailing, adapter ligation, size selection, amplification, and purification.