GSM9171921: 8709-TO-6 / CO+H69 co-culture; Homo sapiens; RNA-Seq
Cells used for single-cell RNA seq were prepared according to PIPseq Single Cell Capture and Lysis User Guide. A cell suspension was prepared in the Cell Suspension Buffer provided from the PIPseqTM T2 3' Single Cell Capture and Lysis Kit v2.0. Cells were added into Pre-templated Instant Partitions (PIPs) and vortexed. Stable emulsions containing captured mRNA were subsequently washed and cDNA synthesis was conducted according to the manufacturer's instructions. cDNA products were submitted to the Vanderbilt University VANTAGE core where whole genome sequencing (WGS) library generation was performed.  cDNA libraries were _x000B_sequenced on an Illumina NovaSeq 6000 instrument at a read depth of 100 million.