GSM8658745: spleen-derived Vps34-deficient Treg cells; Mus musculus; RNA-Seq
Splenic Treg cells were sorted from 10-day-old perinatal control (Foxp3CrePik3c3+/fl) and Foxp3CrePikc3cfl/fl mice and loaded onto the Chromium Controller according to their respective cell counts to target 9,000 single cell GEMs (gel beads in emulsion) per sample. Libraries were prepared using the Chromium Single Cell 3' v3 Library and Gel Bead Kit (10x Genomics)