GSM9177842: NB4, ATRA TREATED, REP1; Homo sapiens; RNA-Seq
Total RNA was subjected to quality control with Agilent Tapestation according to the manufacturer's instructions. To construct libraries suitable for Illumina sequencing, the Illumina stranded mRNA prep ligation sample preparation protocol was used with starting concentration between 25-1000 ng total RNA. The protocol includes mRNA isolation, cDNA synthesis, ligation of adapters and amplification of indexed libraries. The yield and quality of the amplified libraries was analyzed using Qubit by Thermo Fisher and quality was checked by using Agilent Tapestation. The indexed cDNA libraries were normalized and combined, and the pools were sequenced on the Illumina Nextseq 2000 P2 100 cycles kit sequencing run, generating 58 base paired end reads with dual index 10+10 base pairs. Basecalling and demultiplexing was performed using bcl2fastq (v2.20.0) software with default settings generating Fastq files for further downstream mapping and analysis. Total RNA was subjected to quality control with Agilent Tapestation according to the manufacturer's instructions. To construct libraries suitable for Illumina sequencing, the Illumina stranded mRNA prep ligation sample preparation protocol was used with starting concentration between 25-1000 ng total RNA. The protocol includes mRNA isolation, cDNA synthesis, ligation of adapters and amplification of indexed libraries. The yield and quality of the amplified libraries was analyzed using Qubit by Thermo Fisher and quality was checked by using Agilent Tapestation. The indexed cDNA libraries were normalized and combined, and the pools were sequenced on the Illumina Nextseq 2000 P2 100 cycles kit sequencing run, generating 58 base paired end reads with dual index 10+10 base pairs. Basecalling and demultiplexing was performed using bcl2fastq (v2.20.0) software with default settings generating Fastq files for further downstream mapping and analysis.