batch1_WT_HN_Mock_30min_3 (CRX1779670; CRA026850)
We started by extracting Total RNA and using oligodT to get mRNA. This mRNA was then broken down and added to random primers to create the first cDNA strand. We then made a second cDNA strand, using dUTP instead of dTTP. The double-stranded cDNA was processed through end repair, A addition, and adapter ligation. The second strand marked with U was broken down with UDG enzyme. After doing PCR and PCR product recovery, we carried out quality checks on the libraries. The library products were circularized and the circular DNA molecules were duplicated using rolling circle replication, forming DNA nanoballs (DNB). The sequencing was done on the DNBSEQ platform.