Dovetail Omni-C sequencing of Anolis distichus
Dovetail Omni-C libraries were prepared as a service by Dovetail Genomics. Intact chromatin was fixed in formaldehyde, followed by proximity ligation to preserve the 3D conformation of nuclear DNA. Purified, ligated DNA fragments were then used to create high throughput NEBNext Ultra sequencing libraries (New England Biosciences, Ipswich, MA) with Illumina (Illumina, San Diego, CA) adapters. Final OMNI-C libraries were sequenced on an Illumina NextSeq2000 platform