GSM8580457: SW620,caffeine-treated,rep1; Homo sapiens; RNA-Seq
The 1-1.5x106 cells were used for the isolation of total RNA using Extract RNA reagent (Evrogen, Russia). At least two biological replicates were performed for each group. The RNA concentration was determined using the Qubit Fluorometer (Invitrogen, Waltham, MA, USA). The RNA quality was estimated by using the RNA 600 nano kit and Agilent BioAnalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). The RNA Integrity Number (RIN) of the RNA samples was either 8, or above. The NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA), was used to prepare libraries for the RNA-seq in accordance with the manufacturer's guidelines. Seventy-five bp single-end sequencing was performed using the Illumina NextSeq 500 platform (Illumina, San Diego, CA, USA). 75-bp single-end reads