GSM8946876: HEK293T cells, TadA-8e depletion, control sample, tRNA-seq; Homo sapiens; ncRNA-Seq
GFP-positive cells were sorted via flow cytometry and lysed with RNAiso Plus (Takara). Total RNA was extracted using the RNAiso Plus protocol and treated with TurboDNase (Invitrogen) according to the manufacturer's instructions. Genomic DNA was extracted using a commercial kit (Tiangen) following the manufacturer's protocol. tRNA-seq and gDNA-seq libraries were prepared using the QIAseq miRNA Library Kit (Qiagen) and TruePrep DNA Library Prep Kit V2 for Illumina® (Vazyme), respectively, in strict accordance with the manufacturers' guidelines.