GSM9082595: Tdiff.5_dsRNA-seq_IP_Replicate1; Homo sapiens; RNA-Seq
For RNA-seq and dsRNA-seq experiments, RNA was extracted using Trizol and purified using Rneasy or ethanol precipitation. For eCLIP-seq and Riboseq experiments, RNA was purified using lysis buffer and gel-extracted. For RNA-seq experiments, librarires were prepared using the Illumina Stranded mRNA Prep (Ligation). For eCLIP-seq experiments, libraries were prepared using an in-house amplification procedure that closely follows the Illumina Stranded protocol.