Iso-Seq of Arctopsyche grandis: thoracic muscle
RNA was extracted from thoracic muscle of Arctopsyche grandis using a Trizol extraction method, and the extract quality was assessed with a bioanalyzer. The Iso-Seq library was prepared with the bc12 barcode. Barcoded cDNAs were pooled (bc04-bc12) and processed using the Kinnex method. Long-read sequencing was conducted at the DNA Sequencing Center at Brigham Young University (Utah, USA) on a PacBio Revio instrument.