GSM9212503: WT_ATAC-seq_NGO_rep2; Mus musculus; ATAC-seq
FACS-sorted cells were isolated from ovaries and pooled as one replicate. Samples were lysed in 50 μl of lysis buffer (10 mM Tris–HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl2, and 0.1% NP-40, 0.1% Tween-20, and 0.01% Digitonin) on ice for 10 min. Immediately after lysis, nuclei were spun at 500 × g for 10 min at 4 °C to remove the supernatant. Nuclei were then incubated 10 μl of transposition mix (0.5 µl homemade Tn5 transposase, 5 µl 2× TD buffer (10 mM Tris–HCl (pH 7.6), 10 mM MgCl2, and 20% Dimethyl Formamide), 3.3 µl PBS, 0.1 μl 1% digitonin, 0.1 μl 10% Tween-20, and 1 μl water) at 37 °C for 30 min in a thermomixer with shaking at 500 rpm. the transposed DNA was purified with a MinElute kit (Qiagen). DNA libraries were prepared through the omni-ATAC method (PMID: 28846090)