WGS of mus musculus: blood
Whole genome sequencing was performed on WT and deltaF48PKCG mice. 250 ng of Genomic DNA from each sample was fragmented by Adaptive Focused Acoustics (E220 Focused Ultrasonicator, Covaris, Woburn, Massachusetts) to produce an average fragment size of 500 basepairs (bp). Sequencing libraries were generating using the KAPA Hyper Prep Kit (KAPA Biosystems, Wilmington, MA, USA) following manufacturers instructions using 4 cycles of amplification. The quality of the library was assessed using High Sensitivity D1000 kit on a 4200 TapeStation instrument (Agilent Technologies, Santa Clara, CA, USA).Sequencing was performed using the Illumina NovaSeq X Plus Sequencing System (Illumina, San Diego, CA, USA), generating 150 bp paired-end reads to obtain 30x coverage.