RNA-seq of Podarcis muralis
We extract total RNA from samples using QIAGEN's RNeasy Mini Kit. RNA was sent to Novogene (Sacramento, CA) for library preparation and transcriptomic sequencing. mRNA was purified using poly A enrichment followed by cDNA library synthesis and adapter ligation. Purified and quantified libraries were then sequenced on the Illumina NovaSeq X Plus platform to generate paired-end 150 bp reads.