GSM9689543: WT GFP 2; Plasmodium berghei; RNA-Seq
To enrich the gametocytes, mice were pretreated with 50 mg/kg phenylhydrazine were injected with WT GFP and MTP KO parasites. Further, mice were given 2 mg/L sulfadiazine in the drinking water to remove the asexual blood stage of the parasite. Mice with high gametocytemia were sacrificed, and blood was collected. The gametocytes were purified using the nycodenz-density gradient centrifugation. RNA was isolated using Qiagen RNeasy micro kit and treated with DNase I in the column as per manufacturer's instructions. RNA sequencing libraries were prepared with an Illumina-compatible NEBNext® Ultra™ II Directional RNA Library Prep Kit.