GSM8970791: Control 05; Homo sapiens; miRNA-Seq
The total RNA was extracted from the EVs using Total Exosome RNA and Protein Isolation Kit (Cat. 4478545,Invitrogen, CA, USA) using the manufacturer's protocol. The RNA was quantified using Tape Station System (Agilent Technologies, Santa Clara, USA). These samples were then processed for sequencing. The miRNA sequencing library was constructed using the QIAseq miRNA Library Kit (Qiagen, Germany). Briefly, to prepare the miRNA library, total RNA of each sample was used. 3′-and 5′-adaptor ligations were followed by cDNA synthesis, PCR amplification, and gel purification. Quantification was done with Qubit (Thermo Fisher, USA), and quality assessment was done using Agilent D1000 ScreenTape System in a 4150 TapeStation System. The libraries were then sequenced using Illumina NovaSeq 6000 platform (Illumina, USA).