GSM8694457: mRNAseq_OCRRF_rep2; Mus musculus; RNA-Seq
RNA was purified with TRIzol TM Reagent (Invitrogen, 15596026) and Direct-zol RNA microprep (Zymo R2060) RNA-seq libraries were constructed using SMARTer Standard total RNA-seq kit v2 The engineered hyperactive Tn5 transposase that is preloaded with DNA adapters thus removing the need for a separate annealing step. ATAC-seq libraries were prepared for sequencing using standard Illumina protocols