GSM9384848: HCT116_METTL3KD4_rep2; Homo sapiens; RNA-Seq
Total RNA was extracted with the RNeasy Mini Kit. Purified RNA was quantified by a NanoDrop 2000 spectrophotometer, and purity was considered acceptable if A260/A280 values were ≥ 1.8. Library preparation for total RNA-sequencing was done by GENEWIZ. Briefly, 500 ng of total RNA from the indicated sample was used for strand-specific RNA-seq library preparation with rRNA depletion, followed by Illumina paired-end sequencing (2 × 150 bp) to generate approximately 50 million reads per sample.