RNA-seq of directed differentiation of iPSCs into Hepatocyte-like Cells
RNA libraries were prepared with the MGIEasy RNA Library Prep Kit (MGI, China), and coding sequences were enriched using the NEXome Plus Panel v1.0 (Nanodigmbio, China). Library size distribution (~330 bp) and quality were verified using TapeStation DNA ScreenTape (Agilent, USA), and concentrations were measured via Qubit 2.0 (Invitrogen, USA) with the dsDNA High Sensitivity Assay Kit. Paired-end sequencing (150 bp) was performed on a DNBSEQ-G400 platform (MGI, China) using the DNBSEQ-G400 High-throughput Sequencing Set.