GSM8944407: C2C12 Cells, Day 2, fresh, native, H3K4me3; Mus musculus; OTHER
CUT&RUN was performed according to the manufacturer's instructions using the Cell Signaling Technology CUT&RUN kit (#86652), except that cells were incubated with the primary antibody at room temperature for thirty minutes instead of at 4 C for a longer duration to reduce cell death. Libraries were constructed according to the manufacturer's instructions using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (E7645L) using the NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1) (E7600S) as primers