RNA-Seq of sus scrofa
Total RNA from Proximal colon mucosa was extracted and residual genomic DNA was removed using Turbo DNA-free kit. RNA quality and concentration was assesed by gel electrophoresis with RQI quality greater than 8.5. RNA sequencing libraries were prepared with Illumina TruSeq RNA sample prep v2 kits. The combined library pool was denatured and loaded on Illumina NextSeq 500 sequencer using high output flow cell to generate 150-base pair single end reads