WGS Chryseobacterium cupriresistens
Prior to library preparation, theextracted DNA was further concentrated using a centrifugal evaporator. DNA was Forlibrary preparation, the KAPA HyperPrep Kit (Roche, USA) and the Native BarcodingKit (Oxford Nanopore Technologies, UK) were used for conducting the end-repairingand A-tailing, barcode and adapter ligation according to the manufacturers protocol.Nanopore sequencing was conducted using an R10.4.1 PromethION Flow Cell on aP2 Solo sequencer (Oxford Nanopore Technologies, UK).Data acquisition was carried out using MinKNOW (v 24.06.16) (Oxford NanoporeTechnologies) through the Dorado basecaller (v 7.4.14, high-accuracy model), whichalso performed post-processing of the sequence data, including barcode de-multiplexing, adapter trimming, and read-quality filtering with a minimum acceptableQ-score of 7. A total of 630,476 raw reads were obtained, with a read N50 of 2,819bp.