Metatranscriptome of the surface of a deep sea sediment
Extracted quality RNA (A260/280 > 1.8, RIN > 7) was treated with Baseline-ZERO DNase (DB0711K, Epicentre). Libraries were prepared using the Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus kit. Seven samples yielded high-quality data and were retained for downstream analysis. Sequencing was performed on an Illumina NextSeq1000/2000 system using the P2 XLEAP-SBS 2150 kit, generating an average of 100 million reads per sample.