GSM9651229: MIA_Acu_4; Mus musculus; RNA-Seq
Total RNA was extracted from L3‑L5 dorsal root ganglia (DRG) using TRIzol reagent (Invitrogen) following the manufacturer's instructions. RNA integrity and concentration were verified using an Agilent 2100 Bioanalyzer with RIN ≥ 8.0. RNA‑seq libraries were constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina. Poly(A) mRNA was purified from total RNA, fragmented, and reverse‑transcribed into cDNA. Double‑stranded cDNA was ligated with adaptors, amplified, and purified to generate the final library.