RNA-seq of P. fluorescens: Uncoated Rep 1
Total RNA was extracted from Pseudomonas fluorescens using the Trizol reagent. To enrich for mRNA, ribosomal RNA (rRNA) was depleted using the Ribo-Zero Magnetic Kit (Bacteria). RNA integrity was verified using an Agilent 4200 TapeStation. Sequencing libraries were constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina following the manufacturers instructions. The resulting libraries, with an average insert size of approximately 400 bp, were sequenced on an Illumina NovaSeq 6000 platform. Sequencing was performed using a paired-end 150 bp (PE150) strategy to generate a minimum of 3 GB of raw data per sample.