RNA-seq of Col-0 (control for 10X tpp-KO)
Inflorescence apices were dissected to enrich for meristematic and young floral tissues, excluding mature flowers. Tissue was collected in biological triplicates, flash-frozen in liquid nitrogen, and total RNA was extracted using the RNeasy Plant Mini Kit (Qiagen) with on-column DNase I treatment. RNA integrity was confirmed using an Agilent 2100 Bioanalyzer, and RNA quantity was measured using a Qubit fluorometer. RNA-seq libraries were prepared using the Illumina TruSeq Stranded mRNA Library Prep Kit and sequenced on an Illumina NovaSeq 6000 platform to generate ~30 million 150 bp paired-end reads per sample.