GSM9081070: b4Cell_embryo, RNA-Seq Base Edited samples _Replicate2; Bos taurus; RNA-Seq
samples were lysed in TRIsolution (Sigma), followed by phase separation with chloroform. The aqueous phase was collected, and RNA was precipitated with isopropanol, washed with ethanol, and resuspended in nuclease-free water. Five embryos per stage per group were lysed, and mRNA was captured and amplified using the Smart-seq2 v4 kit (Clontech). Amplified cDNA was purified with AMPure XP beads and used for library preparation with the Nextera XT DNA Library Prep Kit (Illumina), followed by multiplexing with Nextera XT Indexes.