complete genome sequence of Bacillus velezensis strain VS05
In this approach, high-quality DNA is extracted from the sample and either physically or enzymatically fragmented. Adapters are then ligated to the ends of these fragments, or tagmentation is performed, combining fragmentation and adapter ligation in one step. The adapter-ligated fragments undergo PCR amplification using primers targeting the adapter sequences. Finally, the resulting library is assessed for size distribution, concentration, and overall quality before proceeding to sequencing.