Genomic and phenotypic data of Acinetobacter baumannii HKAB-1 strain, isolated from sputum of patient with left-sided heart failure
4.1. Sample Collection and Bacterial IsolationThe sputum sample from a patient with a left-sided heart failure suspected to have a bacterial infection was collected for bacterial remuneration analysis. The samples were spread on blood agar and incubated at 37C overnight. The pure cultures were stored at -80C till further processing.4.2 Growth Curve MeasurementColonies of A. baumannii were grown in Mueller-Hinton II Broth (MH2B) liquid medium at 37C for 16-18 h. Cultures were inoculated into fresh 120 ml MH2B medium at an OD600 of 0.05, and cultured at 37C with shaking at 150 rpm. The readout of OD600 was used to plot the bacterial growth curves by the baranyi_without_lag model using the nlsMicrobio package in R 4.3.3 [32] Baty 2014; [33] R Core Team 2021).