Characterisation of a novel PhiKMVvirus APTC-PAO1-PA26 targeting Pseudomonas aeruginosa
Short-read sequencing was conducted on the Illumina platform, using the Illumina NextSeq 550 (Illumina Inc, San Diego, USA) and NextSeq 500/550 Mid-Output kit v2.5 (Illumina Inc., FC-131-1024) at SA Pathology (Adelaide, SA, Australia). The sequencing libraries were prepared using a modified protocol for the Nextera XT DNA library preparation kit (Illumina Inc. FC-131-1024). The genomic DNA was fragmented, after which a low-cycle PCR reaction was used to amplify the Nextera XT indices to the DNA fragments. Sequencing yielded one hundred fifty bp paired end reads.