16S rRNA gene amplicon sequencing of EFW roots in the Experimental Enclosure_pre_replicate 3
Root samples (5-10g) were extracted with the QIAgen PowerSoil Max DNA extraction kit (12988-10) following manufactures guidelines. 16S (V4-V5 region) rRNA gene amplification of gDNA targeted 515F-Y and 926R primers, and 18S (V4 region) rRNA gene amplification of gDNA targeted 565F and 948R primers. Paired end sequencing was performed on an Illumina MiSeq at the National Research Council Canada (Montreal, QC, Canada).