GSM9317487: pACT1-HYF1 25 C; Histoplasma ohiense; RNA-Seq
Cells were collected by filtration, flash frozen in liquid nitrogen, and stored at -80°C prior to RNA extraction. Library preparation and sequencing was performed at SeqCenter as follows: Samples were DNAse treated with Invitrogen DNAse (RNAse free). Library preparation was performed using Illumina's Stranded mRNA prep and 10bp unique dual indices (UDI).  Sequencing was done on a NovaSeq X Plus, producing paired end 150bp reads. Demultiplexing, quality control, and adapter trimming was performed with bcl-convert (v4.2.4).