Apis mellifera gut metagenome
16S metagenomic sequencing libraries were prepared following the Illumina 16S Metagenomic Sequencing Library Preparation guide. The V3-V4 regions were amplified using Bakt_341F and Bakt_805R primers with Illumina adapter sequences. PCR was performed using KAPA HiFi HotStart Ready Mix. Final amplicons were barcoded using the Herculase II Fusion DNA Polymerase Nextera XT Index V2 Kit for dual-indexing.