Nor_PSMPs_3 (CRX1857060)
Microbial DNA was extracted using the HiPure Soil or Stool DNA Kit (Magen, China) according to the manufacturer's protocol. The 16S rRNA gene was amplified by PCR using Q5 High-Fidelity DNA Polymerase (NEB, USA) under standard thermal cycling conditions. PCR products were purified, quantified, and used for library preparation with the Illumina DNA Prep Kit. Libraries were sequenced on the Illumina NovaSeq 6000 platform using PE250 mode.