RNA-Seq of mus musculus
2) Swirl and mix AMPure XP beads after being placed at room temperature for 30min, absorb 65 uL into the upstream products, gently blow 10 times with pipette, transfer the mixed samples to a 1.5mL centrifuge tube, and incubate at room temperature for 5 min. 3) Centrifuge 1.5mL centrifuge tube at low speed instantaneously and place it on a magnetic rack at room temperature for 10min. After the solution is clarified, transfer the supernatant to a new 1.5mL centrifuge tube and discard the magnetic beads; 4) Add 10uL of AMPure XP beads into the upper qing, gently blow with pipette for 10 times and mix thoroughly, incubate at room temperature for 10min; 5) Centrifuge 1.5mL centrifugal tube for a short time and place it in a magnetic rack to separate magnetic beads and liquid, and leave it at room temperature for 5min until the solution is clarified and the supernatant is removed; 6) Keep 1.5mL centrifuge tube always in the magnetic rack, add 200uL 80% ethanol (currently used and prepared) to rinse magnetic beads, incubate at room temperature for 30s and remove the supernatant; 7) Add 200uL 80% ethanol (used now) to rinse the magnetic beads again, centrifuge at low speed instantaneously, and remove the supernatant after incubation at room temperature for 30s; 8) Keep 1.5mL centrifuge tube on magnetic rack, open cover and air dry magnetic bead for 5min; Remove the EP tube from the magnetic rack, add 16uL NF-H2O to elute, vortex oscillation or gently blow with a pipette to mix thoroughly; 9) Centrifuge 1.5mL centrifugal tube at low speed instantaneously and place it in a magnetic rack to separate magnetic beads and liquid. After the solution is clarified, absorb 15uL supernatant into a clean 200uL PCR tube.