GSM8999728: SPF mouse ileum, fresh frozen, Sample 2; Mus musculus; RNA-Seq
The tissue was then dissolved in TRIzol™ (Thermo 15596026) and RNA extraction was performed by the Direct-zol RNA Miniprep Plus kit (Zymo R2071) Library preparation and sequencing was performed by the Harvard Medical School Biopolymers Core using the Ribo-Erase kit for rRNA depletion, the KAPA HyperPrep kit for library preparation, and 150-bp paired-end sequencing on an Illumina NextSeq 500.