Control 12
A Pacific Biosciences HiFi library was prepared according to PN 102-359-000 Version 03 (Pacific Biosciences). Modifications include performing upfront PCR with PacBio 16s primers: 27F (Forward Primer): 5'-GCATCTAGRGTTYGATYMTGGCTCAG-3', 1492R (Reverse Primer): 5'-GCATCRGYTACCTTGTTACGACTT-3' and KAPA HiFi HotStart ReadyMix (Roche) and a purification using AxyPrep Mag PCR beads prior to pooling. Library quality was assessed using the Agilent TapeStation System. Library was quantified using the Qubit dsDNA High Sensitivity kit. The library was sequenced on a Sequel II using Sequel Polymerase Binding Kit 2.103