18s rDNA of Pennisetum giganteum
Total genomic DNA was extracted using the E.Z.N.A. Soil DNA Kit (Shanghai Enzyme linked Biotechnology Co, China). Bacterial and fungal communities were am-plified using specific primer sets: 16S rDNA (338F: 5'-ACTCCTACGGGAGGCAGCAG-3' and 806R: 5'-GGACTACHVGGGTWTCTAAT-3') and ITS (ITS1F: 5'-CTTGGTCATTTAGAGGAAGTAA-3' and ITS2R: 5'-GCTGCGTTCTTCATCGATGC-3'), respectively. PCR products were purified using the AxyPrep DNA Gel Extraction Kit (Axygen, USA) and quantified with a Quantus Fluorometer (Promega, USA). Sequencing libraries were prepared using the NEXTFLEX Rapid DNA-Seq Kit (Bioo Scientific, USA) and analyzed on an Illumina MiSeq PE300 platform (Illumina, USA).