Full length 16S rRNA gene sequencing of soldier fly microbiomes: larval guts
Full length 16S rRNA gene sequencing was conducted with HiFi PacBio Revio technology (Pacific Biosciences, California, USA) at the Australian Genome Research Facility (AGRF, Brisbane, Australia). The 16S rRNA gene was PCR amplified from the extracted genomic DNA of whole larval guts using indexed forward primer F27 (5GCAT/barcode/AGRGTTYGATYMTGGCTCAG3) and reverse primer R1492 (5GCATC/barcode/RGYTACCTTGTTACGACTT3) and sequencing libraries prepared using the Kinnex kit (Pacific Biosciences, California, USA).