GSM9178106: ICL - NE-1; Rattus norvegicus; RNA-Seq
Total RNA extracted  via RNeasy micro kit (Qiagen #74004) The NEBNext Ultra II RNA Library Prep Kit for Illumina & NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) was utilized according to the manufacturer's recommendations. ERCC RNA Spike-In Mix (ThermoFisher, #4456740) was added to normalized total RNA prior to library preparation following manufacturer's protocol.