GSM9831292: Digastric muscle, Sham/Control, rep 1; Mus musculus; RNA-Seq
Total RNA was isolated from muscles using TRIzol reagent (Invitrogen) followed by chloroform extraction, isopropanol precipitation, and DNase treatment. RNA integrity was assessed using an Agilent TapeStation 4200. Poly(A)-selected libraries were prepared from 250 ng RNA using the Illumina mRNA Prep kit and sequenced on an Illumina NovaSeq X Series instrument (2 × 150 bp) to a minimum depth of 40 million reads per sample at the University of Florida Interdisciplinary Center for Biotechnology Research (ICBR) core facility.