GSM9864251: Parse WT kit 5 samples; Mus musculus; RNA-Seq
Parse Biosciences single cell RNAseq capture kit To induce differentiation, NPC cells were plated on dishes coated with 10 μg/ ml laminin (Sigma-Aldrich) and cultured in NeuroCult Basal Medium containing NeuroCult Differentiation Supplement (Stemcell Technologies). After 21 days of induction at 5% CO2 and 37 °C, cells were harvested and performed the single cell RNASeq experiments.