MSQ43: Hi-C protocol: Rep1
Hi-C-libraries were prepared for Illumina sequencing using Kapa Hyper Prep Kit (Roche, #KK8504) and a KAPA Single-Indexed Adapter Set A, (Roche, #KK8701). Biotin pull-down was performed with Dynabeads (Thermo Fisher Scientific, #65601) after the adapter ligation step. Final PCR was approached with 6-8 cycles of amplification. Libraries were sequenced on DNBSEQ sequencing systems (BGI, China).