GSM8727662: IMR90 cells, siZC3HC1 replicate 1; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNeasy mini kit (Qiagen). Libraries were prepared from 500ng of each RNA sample using the NEBNext Ultra II Directional RNA library kit with PolyA enrichment module (New England Biolabs).  Libraries were assessed for quality and fragment size using the Agilent Bioanalyser and sequenced on NextSeq-2000 platform