GSM9001668: ampWC-24H FGF2; Mus musculus; RNA-Seq
Preparation was conducted according to Sample Preparation Demonstrated Protocol provided by 10X Genomics.1 mL of cell suspension was pelleted in Eppendorf tubes by centrifugation (400g, 5min). The supernatant was discarded, and the cell pellet was then resuspended in 1X PBS with 0.04% BSA, followed by two washing procedures by centrifugation (150g, 3min). After the second wash, cells were resuspended in ~500 uL 1X PBS with 0.04% BSA followed by gently pipetting mix 10-15 times. Libraries were prepared using the Chromium Controller (10X Genomics, CA) in conjunction with the single-cell 3' v3 kit. Briefly, the cell suspension was diluted in nuclease-free water according to manufacturer instructions to achieve a targeted cell count of 10,000. The cDNA synthesis, barcoding and library preparation were then carried out according to the manufacturer's instructions. the library was sequenced in the Molecular Genomic Workspace of the Texas A&M Institute for Genome Sciences on a Novaseq 6000 sequencer (Illumina, San Diego).