RNA-seq of Arabidopsis Thaliana siliques from WT (Col-0) and ZFP2-knockout mutant (zfp2-2)
RNA-seq of Arabidopsis Thaliana siliques from WT (Col-0) and ZFP2-knockout mutant (zfp2-2)
Total RNA were extracted from pooled Arabidopsis siliques 12 days after flowering (DAF).  Arabidopsis thaliana seedlings are grown in growth chambers under constrant temperature of 23°C and a photoperiod of 16 h light (100‐150 μmol m−2 sec−1  illumination)/8 h dark. Total RNA extraction was carried out using the Monarch Total RNA Miniprep Kit (New England Biolabs), following the manufacturer's protocols. Subsequently, DNase I (New England Biolabs) was employed to remove genomic DNA, according to the manufacturer's instructions. RNA-seq libraries were generated using NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) for Illumina according to the manufacturer's instruction