GSM9652260: hnRNPC1; Human coronavirus 229E; RNA-Seq
Total RNA was extracted using Trizol reagent Ribosome profiling was performed by CloudSeq Inc. (Shanghai, China) following the GenSeq Ribo Profile Kit manual (GenSeq, Inc.). Briefly, cycloheximide-treated Huh7 cells were lysed with lysis buffer and digested with nuclease. Ribosome footprints were purified by size-exclusion chromatography, and ribosome-protected fragments were size-selected by polyacrylamide gel electrophoresis (PAGE), followed by rRNA depletion. Purified RNA fragments were end-repaired, ligated with 3' adapters and reverse-transcribed to cDNA. The cDNA products were purified by PAGE, circularized, and amplified by PCR. The resulting libraries were purified and sequenced on a NovaSeq platform (Illumina).