GSM9064242: 9395-AS-6; Mus musculus; RNA-Seq
ACPP tumors and normal fat were stored in liquid nitrogen or RNAlater stablization solution until extraction. 30 mg of frozen tissue was dissociated using the BeadBug Homogenizer (Southern Labware, D1030). RNA was extracted using the AllPrep DNA/RNA Mini Kit (Qiagen #80204). RNA concentrations were determined using the NanoDrop 2000 (Thermo Fisher Scientific). Messenger RNA was purified from total RNA using poly-T-oligo-attached magnetic beads. Library construction was performed and completed by Novogene or the UM Advanced Genomics Core