GSM9111456: EGFP+ BN1 cells, unsorted, hypoxia, rep 3; Mus musculus; RNA-Seq
Total RNA were isolated as previously described using RNeasy columns (Qiagen). For transcriptome sequencing, total RNA was extracted by RNeasy columns (Qiagen), and treated with TURBO DNA-free™ KitThermo Fisher Scientific Inc) . The mRNA was isolated and reverse-transcribed to cDNA and amplified using Illumina® Stranded mRNA Prep from Illumina, Inc (San Diego, CA). The library preparation processes such as adenylation, ligation and amplification were performed following the manual provided by the manufacturer(1, 2). The quantity and quality of the libraries were assessed through Bioanalyzer 2100 and Qubit instruments. The procedure of 150 cycle paired-end sequencing in AVITI sequencer followed the manufacturer's manual(3).