In situ microcapsule-entrapped cultivation: activated sludge microbiota 16S rRNA at 0 mg/L tetracycline
Using In situ microcapsule-entrapped cultivation method, genomic DNA was extracted from activated sludge microbiota exposed to 0 mg/L tetracycline. 16S rRNA gene V4 region was amplified by PCR, purified, and used to construct sequencing library.