Whole exome sequencing of anaplastic thyroid cancer of Indian origin
Whole exome sequencing of anaplastic thyroid cancer of Indian origin
The FFPE tumor tissues were collected from ACTREC-TMC The 38 FFPE orphan tumor samples has been subjected to microtome-sectioning where ~5-6 sections of 10 um has been taken based on the tissue abundance for DNA extraction. The DNA extraction from has been performed according to standard protocol of Qiagen's DNeasy FFPE tissue kits. The DNA concentration was measured using Nanodrop 2000c spectrophotometer. Using the Illumina DNA Prep with enrichment Kit, formerly known as the TruSeq DNA Access Library Prep Kit, whole exome sequencing was done on 38 FFPE samples of anaplastic thyroid cancer.