Replictate 3 - IL-10 macrophage
RNA isolation for subsequent RNA sequencing in macrophages was subject to a more rigorous isolation method and additional purification steps, using the miRNeasy isolation kit (Qiagen) as per the manufacturers instructions. The quantity, purity, and quality of RNA was assessed by nanodrop and RNA high sensitivity kit (Qiagen), run on a Bioanalyser Tapestation (4200; Agilent)