GSM9177851: a sample of liver constituent cells from the endothelial cell-specific CTGF KO mouse after pIVCL; Mus musculus; RNA-Seq
After liver perfusion with pronase (#107433; Sigma‒Aldrich) and collagenase (#C5138; Sigma‒Aldrich), hepatocytes were removed via low-speed centrifugation (50 × g), and dead cells and erythrocytes were removed via density gradient centrifugation using Percoll PLUS (GE17-5445-02; Cytiva). The percentage of viable cells in the cell suspension was greater than 70%, and approximately 10,000 viable cells were used for library preparation. For the construction of the scRNA-seq library, a cDNA Kit and Whole Transcriptome Analysis (WTA) Amplification Kit (Becton Dickinson and Co., Franklin Lakes, NJ) were used according to the manufacturer's instructions. Rhapsody WTA libraries were sequenced on the DNBSEQ-G400RS platform (MGI Tech).