GSM8970671: MACS-sorted pancreatic iPSC-derived beta-like cell aggregates, treated with Vehicle H2O for 3 days, biol rep 3; Homo sapiens; RNA-Seq
Total RNA from MACS-purified iPSC-ß cells was isolated using the RNeasy Plus Micro kit (Qiagen, 74034). RNA concentration and integrity were measured using the Bioanalyzer RNA 6000 Nano assay (Agilent, 5067-1511) on the Agilent 2100 Bioanalyzer. Libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs, E7760L) for samples sequenced by Illumina NovaSeq6000 and Novogene NGS Stranded RNA Library Prep Set (Novogene, PT044) for samples sequenced by Illlumina NovaSeq X Plus. Paired-end sequencing (2x151bp) was performed at 200 million reads.