Pacbio HiFi sequencing of Drosphila melanogaster strain A7 adult males
High Molecular Weight (HMW) DNA was extracted from ~200 adult males for the A7 strain following the protocol from (Shukla et al. 2025). HMW DNA samples were sheared via gTUBE (Covaris) and prepared into libraries using the SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences, Menlo Park, CA) with a final size selection step, following the manufacturer's recommendation. Sequencing was conducted on a Pacific Biosciences Sequel II platform at the UC Irvine Genomics High-Throughput Facility.