GSM8797604: Sum159 ITGB4 low clone 1 biol rep 2; Homo sapiens; RNA-Seq
RNA was extracted and purified with RNeasy Micro Kit (Qiagen). Libraries were prepared for RNA-Seq using Watchmaker mRNA Library Prep Kit, according to manufacturer's directions. The Watchmaker mRNA Library Prep Kit first enriches for mature, polyadenylated mRNAs using oligo dT beads. The enriched mRNA is then fragmented, and undergoes 1st and 2nd strand cDNA synthesis, incorporating dUTP to maintain strand information. The double stranded cDNA is then A-tailed, ligated to universal adapters, and amplified using indexed primers to allow sample multiplexing.