merip-TK25-input (CRX1856314; CRA027869)
Break it into fragments of approximately 100nt length using a reagent kit. Divide the RNA into two parts, one of which is used as an input control (i.e. without conducting IP experiments), and directly construct a transcriptome sequencing library to eliminate the background during the process of capturing methylated fragments. The other part of the RNA is enriched with m6A specific antibodies. After capturing m6A modified RNA, antibody elution was performed using magnetic beads to reduce background noise from non-specific binding. Construct strand specific libraries for two RNA samples separately. After the construction of the library is completed, perform quality testing on the library. The library that has passed quality inspection will be sequenced on the machine. submitted_library_strategy: MeRIP-Seq submitted_instrument_model: Illumina HiSeq X Ten