GSM8636207: 293T cells, NT guide, EBSS 48hrs, replicate4; Homo sapiens; RNA-Seq
RNA was harvested using Monarch Total RNA Miniprep Kit Illumina TruSeq Stranded mRNA RNA-seq libraries were prepared at the Crown Genomics institute of the Nancy and Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science. Libraries were prepared using the TruSeq Stranded mRNA-seq kit (Illumina) according to the manufacturing protocol. Briefly, the polyA fraction (mRNA) was purified from 500 ng of total input RNA followed by fragmentation and the generation of double-stranded cDNA. After Agencourt Ampure XP beads cleanup (Beckman Coulter), A base addition, adapter ligation and PCR amplification steps were performed. Libraries were quantified by Qubit (Thermo fisher scientific) and TapeStation (Agilent). Sequencing was done on Novaseq Plus X 1.5B 300 cycles kit, allocating ~50M reads per sample (Illumina; paired end sequencing