GSM9795723: SHAPE MPRA, GFP, Neurite Rep1; Mus musculus; RNA-Seq
RNA was extracted from soma and neurite fractions using a QuickRNA micro kit (Zymo). Targeted sequencing libraries, with UMIs, were constructed through RT-PCR of an amplicon of the reporter transcript used in the MPRA. To minimize effects due to a particular reporter RNA, two reporters RNAs were used. One encoded GFP while the other encoded firefly luciferase.