PacBio sequence reads of the freshly authenticated Candida albicans SC5314 genome (13 kb)
Cells were grown at 37C in YPD, then collected by centrifugation. Zymolyase treatment and genomic DNA purification followed protocols.io.rm7vzb1b4vx1/v1. DNA purification followed protocols.io.rm7vzb1b4vx1/v1. DNA was resuspended in 10 mM Tris-EDTA (pH 8.0), then treated with RNase A (Gold Biotechnology) at 37C for 1 h. Proteinase K (Gold Biotechnology) was added and incubated at 50C for 16 h. The preparation was extracted with phenol/chloroform and DNA precipitated with 2 volumes of isopropanol. Genomic DNA was sheared with a Megaruptor 3 to an average fragment length of 13 kb, then converted into a library with the SMRTBell Express Template Prep kit 3.0. The library was sequenced on a single-molecule real-time (SMRT) cell on a PacBio Revio system. CCS analysis used SMRT Link v13.1 (ccs --min-passes 3 --min-rq 0.99).