GSM9083043: HeLa, GLORI, sgTAF7; Homo sapiens; OTHER
Cells were harvested and total RNA was extracted using TRIZOL reagent (Invitrogen) following the manufacturer's protocols. To isolate mRNA from total RNA, the Dynabeads® mRNA Purification Kit (Invitrogen) was used following the manufacturer's protocols. The RNA concentration was determined using Nanodrop (Invitrogen) by measuring the UV absorbance at 260 nm. Library preparation was then performed using SMARTer Stranded Total RNA-Seq kits v3 Pico Input mammalian (Takara, 634487)