GSM9688402: Mouse brain microvascular fragment (Replicate 3); Mus musculus; RNA-Seq
Brain tissue from adult mice was mechanically and enzymatically dissociated, and vascular fragments were isolated. Sequencing libraries were prepared from 400ng total RNA using the TruSeq stranded mRNA library preparation kit (cat# 20020595, Illumina Inc.) including polyA selection. Unique dual indexes (cat# 20022371, Illumina Inc.) were used.The library preparation was performed according to the manufacturers' protocol (#1000000040498). Paired-end 150bp read length were sequenced.