Illumina RNA sequencing
RNASeq library for gene annotation: total RNA extracted from pooled young leaves, stems and fruits using TRIzol; mRNA enriched with oligo(dT) magnetic beads, fragmented; firststrand cDNA synthesized with random hexamers, secondstrand with DNA polymerase I and RNase H; cDNA purified, endrepaired, Atailed, adapterligated, sizeselected and PCRenriched; sequenced in 2150bp pairedend mode on an Illumina platform