GSM9316880: C&R, OV4, OVARIAN, CD70Pos, EZH2, REP1; Homo sapiens; OTHER
CUT&RUN was performed on cells using the CUTANA™ ChIC/CUT&RUN Kit following the manufacturer's instructions. The following antibodies were used: rabbit anti-H3K4me3; rabbit anti-H3K27me3; rabbit anti-EZH2 ; negative control rabbit IgG . After quantification of the recovered DNA fragments, libraries were prepared using the ThruPLEX®DNA-Seq kit following the manufacturer's instructions, purified with AMPure XP magnetic beads, and quantified using a Qubit fluorometer and a TapeStation, and sequenced on a NovaSeq 6000.