GSM8692794: Control, BTSC53, biol rep 2; Homo sapiens; RNA-Seq
Total RNA was extracted at LuxGen facility (Luxembourg Institute of Health) using Rneasy mini kit (Qiagen).  100 ng of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared at LuxGen facility, using the Illumina Stranded mRNA Ligation kit according to manufacturer.  All libraries have been quantified using a Qubit HS dsDNA kit (Thermofisher) and the library quality check has been performed using a High Sensitivity NGS Fragment Analysis Kit on a Fragment Analyzer System (Agilent Technologies). Indexed libraries were normalized and pooled to be sequenced on an Illumina  Novaseq 6000 sequencer according to manufacturer's instructions with targeted conditions of 2 x 75bp and 25M reads/sample.