Pool-seq of Littorina saxatilis from Venice
Genomic DNA (gDNA) was extracted from intermediate mini-pools, each containing five combined individuals. These mini-pools were subsequently purified using a Zymo spin column protocol. Final maxi-pools for sequencing were created by combining these mini-pools to achieve 105 individuals. The overall pooling strategy followed the general protocols described in Morales et al. (2019). The final pooled samples were prepared using the Illumina TruSeq PCR-free method, targeting 350bp fragments following Covaris fragmentation. The resulting libraries were sequenced at the National Genomics Infrastructure (NGI) in Stockholm on an Illumina NovaSeq X Plus sequencer using a 151nt (Read1)  19nt (Index1)  10nt (Index2)  151nt (Read2) setup for paired-end reads.