WGS of Streptococcus agalactiae: isolate SA_22-25 from tilapia in Peru
Streptococcus agalactiae SA_22-25 was isolated from diseased tilapia and cultured on BHI agar for 24 hours. Genomic DNA was extracted using the Quick-DNA Miniprep Kit (Zymo Research, USA). DNA quality was evaluated with NanoDrop One, Qubit 4.0, and agarose gel electrophoresis. A paired-end DNA library was prepared using the Illumina DNA Prep (M) Tagmentation Kit and sequenced on the Illumina MiSeq platform (2150 bp).