GSM9111763: HUVEC, CTL, 24 hours, replicate 1; Homo sapiens; RNA-Seq
Total RNA isolation was performed with the RNA Mini Kit (Bio&Sell) according to the manufacturers protocol RNA and library preparation integrity were verified with LabChip Gx Touch 24 (Perkin Elmer). Sequencing was performed on NextSeq2000 instrument (Illumina) with 1 x 72 bp single end setup. The resulting raw reads were assessed for quality, adapter content and duplication rates with FastQC (RRID:SCR_014583).