GSM9471609: RM-1 tumors treated with vehicle.; Mus musculus; RNA-Seq
Tumor tissues were dissociated into single-cell suspensions, and CD45⁺ cells were isolated by flow cytometry. CD45⁺ cells were then mixed with tumor cells at a ratio of 3:1 and subjected to single-cell RNA sequencing.Total RNA from each sample was quantified by the NanoDrop ND-1000 and RNA integrity was assessed by standard denaturing agarose gel electrophoresis. Library was performed according to the manufacter's instructions (single cell 3' v2 protocol,SeekOne DD). RNA libraries were prepared for sequencing using standard Illumina protocols