GSM9577981: A6_L3_UDI213; Mus musculus; RNA-Seq
Splenic cDC1s were sorted by FACS from WT, E2A/HEB ΔDC, and Id2 ΔDC mice as live, singlet, lineage-negative (B220⁻NK1.1⁻TCRβ⁻) CD11c⁺MHCII⁺XCR1⁺CD172a⁻ cells with post-sort purity >97%. For stimulated conditions, sorted cDC1s were cultured with heat-killed Listeria monocytogenes-OVA (HKLM-OVA) for 6 hours prior to RNA extraction. RNA libraries for RNA-seq were prepared using NEBNext® UltraTM II RNA Library Prep Kit for Illumina  following manufacturer's protocols.