GSM9064226: Hippocampus of Tau+/+, Control for Tau driven overexpression of Mecp2, biolrep 5; Mus musculus; RNA-Seq
RNA was isolated from samples using Qiazol phase separation followed by cleanup with RNeasy 96 Kit per manufacturer's instruction. Isolated RNA sample quality was assessed by both RNA Tapestation assay and High Sensitivity RNA Tapestation assay (Agilent Technologies Inc., California, USA and quantified by Infinite F Nano+ 200 Pro Tecan® (Tecan, Switzerland). Library construction was prepared using NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible) following the manufacturer's protocol (New England BioLabs Inc., Massachusetts, USA). Final libraries quantity was assessed by Qubit 2.0 (ThermoFisher, Massachusetts, USA) and quality was assessed by TapeStation HSD1000 ScreenTape (Agilent Technologies Inc., California, USA). Final library size was about 250bp with an insert size of about 100bp. Illumina® 6-nt single-indices were used. Equimolar pooling of libraries was performed based on QC values and sequenced on an Illumina® NovaSeq X Plus (Illumina, California, USA) with a read length configuration of 150 PE for 20M PE reads per sample (10M in each direction).