Long-read Kombucha metabarcoding: liquid fraction BHGT day6
PCR products were purified with AMPureXP beads (Beckman Coulter, USA) and prepared using the SQK-LSK109 ligation sequencing kit. Samples were barcoded with EXP-NBD104 and EXP-NBD114 kits (Oxford Nanopore Technologies, UK) following manufacturer protocols. Libraries were sequenced on a single flow cell, quantified with a Qubit Fluorometer, and run for 48 h on the MinION Mk1B platform (R.10 flow cells) using MinKNOW v24.02.8 without real-time base calling.