DNBSEQ-G400 paired end sequencing of SAMD01936993
Total RNA was extracted from budding cells of Fusarium oxysporum f. sp. lycopersici MAFF 103036 grown in liquid culture. Poly(A) selection was performed and strand-specific paired-end RNA-Seq libraries were prepared using the dUTP method (equivalent to Illumina TruSeq stranded mRNA), where the second cDNA strand incorporates dUTP. In this library, Read 1 corresponds to the antisense strand and Read 2 corresponds to the sense strand of the original mRNA. Sequencing was performed at Genewiz (now Azenta Life Sciences). Raw reads (32656569 pairs) were quality-trimmed using Trimmomatic v0.39 with parameters ILLUMINACLIP:DNB-PE.fasta:2:30:10 LEADING:20 TRAILING:20 SLIDINGWINDOW:4:15 MINLEN:36. After trimming, 32117072 read pairs (98.35%) survived and are submitted here. The original raw fastq files are no longer available; only quality-trimmed paired reads are included.