GSM8691772: MpInput; Marchantia polymorpha; OTHER
Genomic DNA extracted with the CTAB method and RNase A treated gDNA fragmented to 200 bp fragments in average and ligated a a truncated Illumina TruSeq adapter (Y adapter). gDNA library is incubated with recombinant protein expressed in E.coli, previously immobilized with Amylose beads. After binding and washing, DNA is eluted and PCR-amplified with Truseq primers, including a double i5/i7 index.