post-SCRaMbLE library of yXL327 from round 2
FACS sorted yeast cells from the post-SCRaMbLE library were grown in SC-His media. HMW gDNA was extracted from the pool of cells. Long range PCR of the full synthetic module was performed using LA Taq Hot Start (TaKaRa, Shiga Japan). The pool of amplicons was prepped for sequencing with NEBNext Companion Module and the ligation kit SQK-LSK109 or SQK-LSK114. The library was sequenced on Flongle (FLG001 or FLG114) using MinION Mk1B. The data was collected with the latest version of MinKnow (22.05.5  23.04.6).