GSM9864055: WNT, 50 h, rep 2; Homo sapiens; RNA-Seq
Cells were dissociated with Accutase, and total RNA was extracted using the Invitrogen RNAqueous-Micro Total RNA Isolation Kit according to the manufacturer's instructions. RNA quality was assessed using a NanoDrop spectrophotometer, agarose gel electrophoresis, qPCR, and an Agilent 2100 Bioanalyzer before library preparation. RNA sequencing libraries were prepared by Novogene Co. using a poly(A)-enriched mRNA library preparation protocol. Libraries were sequenced on the Illumina NovaSeq X Plus platform to generate paired-end 150-bp reads.