RNA-seq of Streptomyces venezuelae adpA deletion strain
RNA-seq of Streptomyces venezuelae adpA deletion strain
the liquid MYM medium supplemented with trace elements was inoculated to the final OD of 0.001 with the spores of S. venezuelae strains  and incubated for 12 and 20 hours at 30C with shaking (220 rpm). The cultures were prepared in two independent repetitions per strain using standard 250-ml flasks (flat bottom, w/o baffles) containing 50 ml of the medium. At the time of collection (12 and 20 hours) the culture suspensions were mixed with the stop solution and immediately spun down. After discarding the supernatants the cell pellets were stored at -80C for further use.  Library preparation was performed by Genewiz (Germany).