GSM8489214: WT,replicate1,snRNA-seq; Rattus norvegicus; RNA-Seq
The anterior horn of lumbar spinal cord from 40-day-old COXⅢLSL and COXⅢNeuN-Cre rats were used for the isolation of the nuclei, based on methods reported in previous studies15. Nuclei from frozen anterior horn of lumbar spinal cord tissues were isolated using Shbio Nuclei Isolation Kit (SHBIO; Shanghai, China). For cDNA library preparation, 10000 nuclei were used for the Single-cell RNA (scRNA) library construction. The single-nuclei suspension (700-1200 nuclei per ml as determined by the CellDrop FL Cell Counter) were loaded into microfluidic chip of Chip A Single Cell Kit v2.1 (MobiDrop no. S050100301) to generate droplets with MobiNova-100. Each nucleus was encapsulated into a droplet which contained a gel bead linked with up to millions oligos (cell unique barcode). The mRNAs were captured by cell barcodes with cDNA amplification in droplets. According to the manufacturer's introduction, scRNA-seq libraries were constructed using the High Throughput Single-Cell 3' Transcriptome Kit v2.1 (MobiDrop no. S050200301) and sequenced on illumina platform by Annoroad Gene Technology Co., Ltd.