GSM9001283: Foxc1 ab1; Mus musculus; ChIP-Seq
Prior to cross-linking media were replaced with fresh DMEM supplemented with 10% FBS. To crosslink proteins to DNA, paraformaldehyde (PFA) solution was added to cell media to a final concentration of 6%. After 10 min incubation at room temperature remaining PFA was quenched by addition of 10X glycine solution. Glycine solution was added to each plate and incubated for additional 5 min at room temperature to quench the remaining PFA. Cross-linked chromatin was prepared and immunoprecipitated using Simple ChIP plus Enzymatic Chromatin IP Kit (New England Biolabs). Chromatin was reverse-crosslinked using Proteinase K digestion and purified according to kit manufacturers instructions. ChIP-seq libraries were prepared using the NEBNext Ultra II Library Prep Kit (New England Bioloabs). Library preparation and sequencing was performed at the BRC Sequencing Core (University of British Columbia, Vancouver, BC).