BASP1 drives cisplatin resistance through LIN7A mediated epithelial-mesenchymal transition in OSCC. Bulk RNA seq
BASP1 drives cisplatin resistance through LIN7A mediated epithelial-mesenchymal transition in OSCC. Bulk RNA seq
SCC9 CisR WT and  BASP1 KD cells were cultured in cell culture conditions in DMEM-F12 (10%FBS) . Cells were trypsinized and subjected to RNA isolation. Total RNA was isolated using Direct-Zol RNA miniprep (R2052) RNA isolation kit. To prepare an RNA-Sequencing library, 500ng of total RNA was used to isolate mRNA using magnetic beads (PolyA mRNA Isolation Module, NEB Cat No# E7490L). The RNA-Seq library was then prepared using an mRNA library preparation kit (NEB Cat No# E7770L) strictly following the vendorâ€™s recommended protocol. Following library preparation, the library concentration was measured using Qubit 2.0 (Invitrogen), and the appropriate fragmentation sizes were confirmed using Bio-analyzer (Agilent Cat No# 5067-5582). 