Oxford Nanopore Technology
GenomicDNA libraries were created from ultra-high molecular weight DNA from zebrafish cell culture using the Oxford Nanopore sequencing kit SQK-ULK114. Each library was loaded onto a FLO-PRO114M flow cell and run for 72 hours or more with two subsequent loadings at 24-hour intervals. The libraries were sequenced on a PromethION (Oxford Nanopore) running MinKNOW software version MinKNOW 24.02.19. Base calling and modified base detection (5mC, 5hmC) were performed using Dorado 7.3.11.