GSM8969810: K-562 and HEK293T VASA-seq plate 1; Homo sapiens; RNA-Seq
Single cells were deposited in a 384-well plate using a BD FACSymphony S6 equipped with a 130 um nozzle at 14 psi. Single cells were lysed in their respective lysis buffer (e.g., STORM-seq, VASA-seq, or Smart-seq3xpress). Passage matched K-562 and HEK293T cells were deposited same day for both STORM-seq and VASA-seq. Libraries were constructed as previously described for Smart-seq3xpress (https://doi.org/10.1038/s41587-022-01311-4) or by Single Cell Discoveries following the VASA-seq protocol (https://www.scdiscoveries.com/services/vasa-seq/; https://doi.org/10.1038/s41587-022-01361-8). For STORM-seq, libraries were constructed following https://doi.org/10.5281/zenodo.15178455.