GSM8766255: WT Jurkat, Unstimulated, No inhibitor, rep3; Homo sapiens; RNA-Seq
RNA was extracted from cells resuspended in Trizol (Invitrogen) using a Direct-zol RNA MiniPrep Kit (Zymo Research), including on-column DNaseI treatment. Library preparation was performed with 140 ng total RNA using the Illumina Stranded Total RNA Prep with Ribo-Zero Plus Kit (Illumina) and IDT for Illumina RNA UD Indexes Set A, Ligation (96 Indexes, 96 Samples). Sequencing libraries were quantified by real-time PCR using KAPA Library Quantification Kit for NGS (Roche) and assessed for size distribution on a Fragment Analyzer (Agilent) to confirm absence of adapter dimers and unligated library molecules.