GSM9543190: MDLBM; Mus musculus; RNA-Seq
Splenocytes were collected from 24M BL6 mice by gently dissecting the spleen and diluted into an equal volume of Dulbecco's PBS + 2% FBS (StemCell Technologies, 07905). Similarly Bone marrow cells were collected by flushing the femur and humerus bones with 1x PBS by a syringe. To facilitate removal of any remaining RBCs, a lysis step was performed (Biolegend, 420302). Briefly, lysis buffer (1X) was added to the cell suspension and incubated for 5 minutes, with periodic inversion. Cells were then centrifuged at 350 rcf for 10 minutes, and cells  were washed twice with PBS + 2% FBS. Libraries were constructed according to manufacturer's specification for the 10X Genomics 3' single-cell gene expression analysis, with multiplexing