GSM9578138: Hippocampus, WT, rep2; Mus musculus; RNA-Seq
Total RNA was extracted from dissected hippocampal tissue using TRIzol reagent (Thermo Fisher Scientific) according to the manufacturer's instructions. RNA concentration and integrity were assessed using spectrophotometry and capillary electrophoresis. RNA sequencing libraries were prepared from total RNA using a poly(A) RNA enrichment strategy followed by strand-specific library construction. Libraries were generated according to the manufacturer's protocol and subjected to paired-end sequencing.