MHiC-p1 (CRX1807100)
The whole body was fixed with formaldehyde, lysed, and cross linked DNA digested overnight with DpnII. Sticky ends were biotinylated and proximity ligated to form chimeric junctions, then sheared to 300 500 bp fragments. These chimeric fragments, representing original cross linked long distance interactions, were processed into paired end libraries and sequenced on Illumina NovaSeq 6000.