HiC sequencing of F333 accession
Young leaves of mung bean plants were fixed and lysed, after which the cross-linked DNA was digested with MboI enzymes overnight. The enzymes were inactivated and cohesive ends were filled in by adding biotin-labelled dCTP. After proximity ligation in blunt-end ligation buffer, the cross-linking was reversed and DNA was purified for Hi-C library construction