GSM9762051: Old Brain ECk4KO replicate 2; Mus musculus; ATAC-seq
Brain endothelial nuclei were purified from mouse brain tissue. Nuclei were subjected to Omni-ATAC-seq library preparation. Briefly, nuclei were isolated, permeabilized, and incubated with Tn5 transposase to generate sequencing-compatible chromatin fragments. Libraries were amplified, indexed, purified, quality controlled, and sequenced on an Illumina platform. For Omni-ATAC-seq, purified endothelial nuclei were subjected to Tn5 transposition followed by PCR amplification and sequencing library preparation.