16S full length (V1-V9) of human: stool sample 191
The resultant 16S amplicon pool (~1500bp) was used as a template to construct a SMRTbell library using Express Template Prep 3.0 (Pacific Biosciences) as per the manufacturer's Template Preparation protocol. The amplicon library quality was assessed using the Qubit 3.0 Fluorometer (Thermo Fisher Scientific Inc.) and the Agilent Femto Pulse System (Agilent Technologies). The amplicon library was sequenced using the Sequel II sequencing kit 2.0 (Pacific Biosciences), with 0.5-h pre-extension and 2-h movie time on the Sequel IIe system.