GSM9864435: Wild-type HEK293T replicate 1; Homo sapiens; OTHER
Total RNA was extracted using TRIzol RNA extraction reagent. RNA integrity was assessed using the Agilent Bioanalyzer 2100 system. Messenger RNA was purified from total RNA using poly-T oligonucleotide-attached magnetic beads. After fragmentation, first-strand complementary DNA was synthesized using random hexamer primers, followed by second-strand complementary DNA synthesis. Libraries were prepared by end repair, A-tailing, adapter ligation, size selection, amplification, and purification. Library quality was assessed using Qubit, real-time polymerase chain reaction, and the Bioanalyzer, followed by paired-end sequencing on an Illumina platform.