N+Fe_1 (CRX1901820; CRA028822)
The PCR amplification cycling conditions were as follows: initial denaturation at 95 for 3 min, followed by 35 cycles of denaturing at 95 for 30 s, annealing at 55 for 30 s and extension at 72  for 45 s, and single extension at 72  for 10 min, and end at 10.