GSM9104326: PCPAi; Homo sapiens; RNA-Seq
The tumor samples used for sc-RNA sequencing were collected from 2 female patients who were diagnosed with TNBC and axillary lymph node metastasis. After the conbination treatment of pembrolizumab, carboplatin, paclitaxel and anthracycline, these two patients were divided into two groups based on treatment efficacy: sensitive and insensitive . Library was performed according to the manufacter's instructions. Briefly, immune cells were resuspended in the master mix and loaded together with partitioning oil and gel beads into the chip to generate the gelbead-in-emulsion (GEM). The poly-A RNA from the cell lysate contained in everysingle GEM was retrotranscripted to cDNA, which contains an llumina R1 primel sequence, Unique Molecular ldentifier (UMl) and the 10x Barcode. The pooled barcoded cDNA was then cleaned up with Silane DynaBeads, amplified by PCR and the apropiated sized fragments were selected with SPRlselect reagent for subsequent library construction.