GSM9213464: HET_237_ATAC_rep2; Homo sapiens; ATAC-seq
For ATAC-seq, live cells were rinsed with 1X PBS and lysed with cold 50 mL lysis buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630). After centrifugation at 500 x g for 10 min at 4°C, the resulting nuclei pellet was re-suspended in 50 mL tranposase reaction mix (Illumnia #20034197) and incubated at 37°C for 30 min. For ATAC-seq, Zymo ChIP DNA Clean and Concentrator-5 kit (Zymo Research D5205) was used to purify the DNA, which was then amplified with Nextera primers (1.25 M) using NebNext High-Fidelity 2X PCR Master Mix (New England Biolabs MO541). The final libraries were purified by gel excision of 155-250 bp fragments and single-end sequenced.