GSM9864699: HCT116 cells, DMSO control 3, hypoxia; Homo sapiens; RNA-Seq
Total RNA was harvested using TRIzol reagent (Invitrogen, USA) according to the manufacturer's protocol. RNA integrity and purity were assessed using an Agilent 2100 Bioanalyzer. RNA libraries for RNA-seq were prepared using the SMARTER mRNA-Seq Library Prep Kit following the manufacturer's protocols. Strand-specific libraries were constructed.