GSM8831654: Normal 3; Homo sapiens; RNA-Seq
RNA was isolated using TRIZOL reagent (Invitrogen, MA, USA) Poly-A pull-down was used to enrich for mRNAs, and libraries were prepared using the Illumina TruSeq RNA kit. Libraries were pooled and sequenced on an Illumina HiSeq2000 machine with 150 bp paired-end reads. RNA libraries were prepared for sequencing using standard Illumina protocols