GSM8552770: mRuby2 control cells, biol rep 1; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol.Approximately 1 µg of total RNA was used for the construction of sequencing libraries. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.