GSM8517036: Replicate 2 for P28 cortex ALM interneuron; Mus musculus; ATAC-seq
For P28 ALM (Dlx6a-cre::INTACT) scATAC-seq data, brains were collected, sectioned coronally on a stainless-steel mouse brain slicer (Zivic Instruments), and regions of interest were dissected in ice-cold ACSF. Tissue was then transferred to a Dounce homogenizer containing lysis buffer (10 mM Tris-HCl, 10 mM NaCl, 3 mM MgCl2, 0.01% Tween-20, and 0.01% IGEPAL CA-630, 0.001% digitonin). Tissue was homogenized with 10 strokes of pestle A, 10 strokes of pestle B, and incubated for 5 min on ice before being filtered through a 30 μm filter and centrifuged at 500g for 10 min at 4 °C. The pellet was resuspended in 1% BSA in PBS for sorting for GFP+ nuclei. Nuclei were sorted into diluted nuclei buffer (10x Genomics) on a Sony SH800S cell sorter with a 100 µm chip. The scATAC-seq library was prepared using the 10x Genomics platform with the Chromium Single Cell ATAC Library & Gel Bead Kit v.1.0 (PN-1000111), Chromium Chip E Single Cell kit (PN-1000156) and Chromium i7 Multiplex Kit N, Set A (PN-1000084) as per manufacturer's instructions. Libraries were sequenced using a Nova-Seq S2 100 cycle kit (Illumina).