GSM8944921: 17cre-id2; Mus musculus; RNA-Seq
Mice were immunized with MOG/CFA to induce EAE. CD4+ YFP+ cells from spleen at the peak of disease were sorted on an AriaIII cell sorter into 15-ml tubes containing complete media, counted, and placed on ice. All samples were spun down at 500g for 10 min. The supernatant was removed, and cells were lysed by Trizol. RNA libraries for RNA-seq were prepared using NEBNext® UltraTM II RNA Library Prep Kit for Illumina  following manufacturer's protocols.