6_1_3 (CRX2184527)
Total RNA was isolated using RNA easy Isolation Reagent and checked for quality with a Qubit fluorometer and QSEP100 system. Sequencing libraries were prepared per DNBSEQ standard protocols.  After end repair, A_tailing, and adapter ligation, the products were PCR amplified and circularized. Library quality was verified using an Agilent 2100 Bioanalyzer. Circularized DNA was amplified via rolling circle amplification to generate DNA nanoballs . Sequencing was performed on the DNBSEQ platform using combinatorial Probe Anchor Synthesis  technology.