Metabarcoding of Tomato Root Endophytic Communities Under Humic Substances and Osmotic Stress Treatments
The bacterial rrn operon was amplified from tomato root endophytic microbial DNA using a Nanopore metabarcoding approach. Library prep was then performed using the SQK-LSK114 ligation kit and the sequencing was carried out using the current V14 sequencing chemistry (R10.4.1 Flongle flowcells). Raw pod5 files were basecalled using Dorado (sup 4.2.0 model).