GSM8516332: iWAT_snRNA_LAKO; Mus musculus; RNA-Seq
Frozen iWAT (pooled from 10 mice per group) was homogenized, and nuclei were counted with the use of a LUNA-FL Automated Fluorescence Cell Counter. the nuclei were isolated by flow cytometry. Barcoded cDNA libraries were constructed from the sorted nuclei with a 10X Genomics Chromium Instrument and cDNA synthesis kit (1000268 and 1000127, 10X genomics)