GSM9080901: P6_WT_M_Jejunum_L42150-2; Mus musculus; RNA-Seq
The small intestine was dissected and a 2 cm segment of the region of interested was collected and opended. The flat tissue sheet was in HBSS with EDTA for ~15 mins and the epithelial cells were released by using mechanical force. Epithelial cells were washed 2 times in HBSS then snap frozen in liquid nitrogen. Total RNA was extracted using trizol. RNA-sequencing libraries were made from ≥500ng of purified mouse intestinal cells using the Kapa Stranded mRNA-seq kit