16s rRNA gene sequencing: rearing water
30ng qualified DNA template and the 16S rRNA fusion primers are added for Polymerase chain reaction (PCR). All PCRproducts are purified by Agencourt AMPure XP beads, dissolved in Elution Buffer and eventually labeled to finish libraryconstruction. Library size and concentration are detected by Agilent 2100 Bioanalyzer. Qualified libraries are sequencedwith DNB platform according to their insert size