P1.T3_GEX Multiome ATAC + Gene Expression
Following 10x Genomics protocol, we loaded around 16,000 nuclei per sample per channel of the Chromium Next GEM Chip J for processing on the 10x Chromium Controller (10x Genomics, Pleasanton, CA, USA). This was followed by transposition or cDNA generation and library construction according to the manufacturer's instructions (Chromium Next GEM Single Cell Multiome ATAC + Gene Expression User Guide, Rev F).  The resulting libraries were then normalized and pooled for sequencing on two NovaSeq SP-100 flow cells (Illumina, Inc., San Diego, CA, USA).