GSM8516702: AtcS_Mutant_Day4_rep5; Treponema denticola; RNA-Seq
RNA extraction via TRIzol method. Total RNA quantity and integrity were determined using an Agilent Bioanalyzer RNA Pico assay. All samples passed quality control assessment with RNA Integrity Numbers (RIN) above 9. Ribosomal reduction was then performed on all samples using Illumina's Ribo-Zero rRNA Removal Kit. The resulting depleted RNA was assessed using an Agilent Bioanalyzer RNA Pico assay to confirm efficient rRNA removal. Kit to sequencing libraries - Illumina® Stranded Total RNA Prep, Ligation with Ribo-Zero Plus For Sequencing - NextSeq 1000/2000 P1 Reagents (300 Cycles. Read length is 150bp x 150bp for sequencing, Paired End.