GSM9651253: HCT116, shMYB + shHDAC2 + shFOXA2, biol rep 2; Homo sapiens; RNA-Seq
Total RNA was extracted using RNA-spin kit (INTRON) according to the manufacturer's instructions. Sequencing libraries were prepared using TruSeq RNA Sample Preparation kit v2 (Illumina Inc., USA). After pooled libraries were denatured, sequencing of each library was carried out using the 100 bp paired-end mode of the TruSeq Rapid PE Cluster Kit and TruSeq Rapid SBS Kit with HiSeq 2500 (Illumina Inc., USA).