GSM9082750: DMSO-treated, replicate 3; Homo sapiens; RNA-Seq
Total RNA was extracted using the Qiagen QIAsymphony RNA kit following the manufacturer's instructions (Qiagen, Hilden, Germany). RNA samples were quantified using a Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA), and RNA integrity was checked using an Agilent TapeStation 4200 (Agilent Technologies, Palo Alto, CA, USA). RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using the manufacturer's instructions (NEB, Ipswich, MA, USA).