GSM9111434: Wild-Type,Muscle,Time60 r4; Pteronotus mesoamericanus; RNA-Seq
Skeletal muscle tissue was dissected in the field immediately post-euthanasia and flash frozen in LN2. Samples were stored at –80°C until processing. Total RNA was extracted from 10mg of tissue following the manufacturer's protocol for the Maxwell® RSC simplyRNA Tissue Kit (Promega, Cat# AS1340). RNA integrity was confirmed using an Agilent Bioanalyzer prior to library preparation. RNA libraries for RNA-Seq were prepared using NEBNext Ultra II kit following manufacturer's instructions.