RNASeq of larval Pacific cod after experimental exosure to suboptimal temperatures and acidification; homogenized whole-body larvae
RNAlater-preserved larval fish were homogenized, then RNA was isolated following the TRIzol Reagent protocol for total RNA. Non-directional RNA-Seq libraries were prepared with the NEBNext Ultra II RNA Library Prep Kit for Illumina, and paired-end sequencing was conducted on one lane of a NovaSeq 6000 Sequencing System with 150-bp read length.