GSM8517614: AK_P6_atac; Homo sapiens; ATAC-seq
1 ml of 1 XHB buffer was added into the tissue sample, after grinding and filtering, filtrate was centrifuged at 4℃ 500×g for 5 min, and then supernatant was discarded, density gradient centrifugation for 10 min, nucleus suspension was obtained. 50,000 nuclei were taken and resuspended in Tn5 transposase reaction mixture. The transposition reaction was incubated at 37℃ for 30 min. Equimolar Adapter1 and Adatper 2 were added after transposition, PCR was then performed to amplify the library. After the PCR reaction, libraries were purified with the AMPure beads and library quality was assessed with Qubit. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumina) according to the manufacturer's instructions.