GSM9791701: CA3; Homo sapiens; miRNA-Seq
Total RNA was extracted using Trizol reagent The small RNA libraries were constructed with GenSeq® Small RNA Library Prep Kit (GenSeq, Inc.) by following the manufacturer's instructions. Briefly, 3' and 5' adaptors were successively ligated to RNA samples. Then, the adaptor-ligated RNA was reversely transcribed into cDNA, followed by PCR amplification. After amplification, the cDNA libraries were size-selected for miRNA fractions before sequencing and then sequenced in platform.