RNA-Seq of Tachyglossus aculeatus: adult female ovary (active)
Total RNA was isolated using TRIzol according to the manufacturer's instructions. RNA was quantified and quality-checked using the Agilent 2100 Bioanalyzer. RNA was sheared using Covaris S220 Focused-ultrasonicator. Libraries were prepared using the Illumina Ribo-Zero Plus rRNA Depletion Kit and the KAPA Stranded RNA-seq Library Preparation Kit. Libraries were sequenced on the Illumina HiSeq by the Australian Cancer Research Foundation generating 2x100bp reads.