Philodina roseola de novo whole genome sequencing
Genomic DNA was isolated from Philodina roseola isolate P_roseola_NVCRC_MDU_01 using the QIAamp DNA Mini Kit (Qiagen). DNA quantity and quality were assessed using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific). Sequencing libraries were prepared using the KAPA HyperPlus Kit (Roche) according to the manufacturer's instructions. Libraries were purified using KAPA Pure Beads (Roche), and library size distribution was evaluated using the Agilent 2100 Bioanalyzer with a High Sensitivity DNA Chip. Whole-genome sequencing was performed on the Illumina NovaSeq 6000 platform, generating 2  151 bp paired-end reads for de novo genome assembly and annotation.