hws_M12h_rep2 (CRX1899917; CRA028769)
Total RNA was isolated from cordycepin-treated or untreated seedlings using TRIzol Reagent (Ambion). The quantity and quality of the isolated total RNA were assessed using a BioDrop Spectrophotometer. 2 ug Total RNA (contains 2 ul spike-in mix) was oxidized in the presence of 25 mM freshly prepared sodium periodate (Sigma) in 1x Borate buffer (25 mM borax, 25 mM boric acid, pH 8.6) for 30 min at room temperature. After oxidation, the solution was cleaned by prepacked Sephadex G-25 resin, RNA was ethanol precipitated and further subjected to small-RNA cloning with the QIAseq miRNA Library Kit (QIAGEN).