S3-IP (CRX1511813; CRA022804)
Cells were fixed with 1% formaldehyde (10 min,RT) and crosslinking terminated by 0.125 M glycine. After lysis with cell lysis buffer, nuclei were pelleted by centrifugation (2000g, 5 min) and treated with nucleus lysis buffer. Chromatin was fragmented via sonication. The processed chromatin was divided: 10% preserved as input control, 80% immunoprecipitated with anti-DYKDDDDK tag antibody (Proteintech, 20543-1-AP; IP group), and 10% incubated with rabbit IgG (Cell Signaling Technology, 2729S; IgG control). DNA libraries were constructed using VAHTS Universal DNA Library Prep Kit for Illumina V3 (Vazyme, ND607), with size selection (200-500 bp) and quantification prior to sequencing.