GSM8635860: Parp7 siRNA1 CEBPb Replicate 1; Mus musculus; OTHER
3T3-L1 cells were cultured and treated as described above in 15 cm diameter plates.  The cells were transfected with siRNAs targeting Parp7 or a universal control 48hrs before the addition of the differentiation cocktail. The cells were then differentiated for 24hrs Cells were harvested by trypsinization and counted using the BioRad TC20 automated cell counter. 100,000 cells were collected and pelleted by centrifugation. Cut & Run was preformed using the Epicypher kit (CUTANA ChIC / CUT&RUN Kit) according to manufacturer protocol. In short, the cells were bound to beads, permeabilized using 0.1% digitonin and incubated with 0.5µg antibodies. n= 2 biological replicates.