GSM9471946: PY CYP7a1-KO 1; Danio rerio; RNA-Seq
Whole zebrafish larvae were euthanised in tricaine, pooled into microcentrifuge tubes, snap-frozen in liquid nitrogen, and stored at −80°C. Total RNA was extracted using the Direct-zol RNA Miniprep kit following homogenisation in TRIzol reagent, according to the manufacturer's instructions. RNA concentration and integrity were assessed using the Agilent TapeStation system. RNA sequencing libraries were prepared using the Lexogen QuantSeq 3′ mRNA-Seq library preparation protocol according to the manufacturer's instructions. Libraries were generated from total RNA and are enriched for the 3′ end of polyadenylated transcripts.