TPH Enriched Culture  Noblejas SynCom
A synthetic microbial consortium (SynCom) was constructed by pooling genomic DNA from five hydrocarbon-degradating bacterial isolates (Pseudomonas, Novosphingobium, Delftia, Achromobacter, and Rhodococcus). DNA was extracted using the NucleoSpin Microbial DNA kit and quantified via Qubit fluorometry. A multi-isolate library was prepared using the Oxford Nanopore Ligation Sequencing Kit (SQK-LSK110) from an equimolarly pooled DNA mixture. Sequencing was performed on a MinION R9.4.1 flow cell, with base-calling conducted using GUPPY v6.0.7 via MinKNOW v22.03.6.