Amplicon Sequencing of wastewater sample
The 16S rRNA genes from the extracted DNA were amplified and sequenced at the University of Minnesota Genomics Center (UMGC) using the Illumina MiSeq platform with paired-end sequencing (2300 bp). Data processing, including filtering and rarefaction, was carried out at the Minnesota Supercomputing Institute (MSI) using the QIIME2 (version 2019.10) pipeline. Sequences were clustered de novo at 99% similarity and assigned to operational taxonomic units (OTUs) based on the SILVA 138 rRNA reference database.