16S metabarcoding of koi food pellets
To identify the microbiome, metabarcoding was used. Samples were sent to Tools Biotech for Illumina MiSeq sequencing of the V3/V4 region of the 16S rRNA gene (forward primer with the adapter region underlined: 5- TCG TCG GCA GCG TCA GAT GTG TAT AAG AGA CAG CCT ACG GGN GGC WGC AG-3; reverse primer with the adapter region underlined: 5- GTC TCG TGG GCT CGG AGA TGT GTA TAA GAG ACA GGA CTA CHV GGG TAT CTA ATC C-3)