GSM9001466: small 4N clone derived from DLD1, biological replicate S3; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNeasy extraction kit. mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and sequenced using an Illumina NovaSeq platform. The first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library.