GSM8969400: UXGL_ULIX_2; Homo sapiens; RNA-Seq
Stable naive hESC lines were used for the bulk RNA-seq experiments. Human naïve ESCs were dissociated to single cells. 6x10^5 cells were resuspended in 600 µl RLT buffer (Qiagen) and snap frozen on dry ice for later processing. RNA was isolated using the RNAeasy Mini Kit (Qiagen, 74104) , QIAShredder DNase digest (Qiagen, 79654), automated on a QIAcube (Qiagen) according to the manufacturer's instructions. The RNA library prep was performed using the mRNA polyA (NEB) kit, before paired-end 100 bp sequencing on a NovaSeq2.