Hic reads of Malus robusta
In situ Hi-C libraries were prepared from leaf tissue by formaldehyde crosslinking to preserve chromatin interactions. DNA was digested with HindIII, end-repaired with biotin-labeled nucleotides, proximity-ligated, purified, and sequenced on the Illumina NovaSeq 6000 platform (PE150) to support chromosome-level genome scaffolding.