GSM9386270: RNA_4C_WT_mCherry-injection_rep2; Mus musculus; RNA-Seq
For RNA-seq of early embryos, 12-20 embryos were pooled per sample. The zona pellucida was removed using acidic Tyrode's solution. Samples were washed three times in 0.2% BSA/PBS, then transferred to 0.1-ml tubes with 0.5 μl residual fluid. cDNA libraries were prepared using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) for MII oocytes, SMART-Seq v4 Ultra-Low-Input RNA Kit for Sequencing (Takara) or its successor, SMART-Seq mRNA LP (Takara) for E2C and 4C embryos. Libraries were sequenced with paired-end reads on the NextSeq 500, HiSeq 2000, or NovaSeq X Plus (Illumina).