GSM9241943: Fast_24h; Homo sapiens; RNA-Seq
Total RNA was isolated using the QIAzol lysis reagent (79306, Qiagen, Germantown, MD, USA). RNA quality was assessed using an Agilent 2100 bioanalyzer (Agilent Technologies, Amstelveen, The Netherlands), and RNA quantification was performed using an NanoDrop-2000 spectrophotometer (ND-2000, Thermo Fisher Scientific). Libraries were prepared from total RNA using the NEBNext Ultra II Directional RNA-Seq Kit (New England BioLabs, Hitchin, UK). mRNA isolation was performed using a Poly(A) RNA Selection Kit (LEXOGEN, Wein, Austria). The isolated mRNAs were used for cDNA synthesis and shearing by following the manufacturer's instructions. Indexing was performed using Illumina indexes 1–12.