GSM8552763: MANF overexpression rep 1; Caenorhabditis elegans; RNA-Seq
For each group, 250-500 worms were collected and washed 3x with K-medium before flash-freezing in 1 ml Qiagen RLT buffer containing B-ME. Samples were then thawed and disrupted with a bullet blender (bead beater) at power 8 for 30 second intervals with 90 second rests on ice between. Worm lysates were then diluted with 70% ethanol and loaded onto RNeasy columns. RNA was isolated using the manufacturer protocol (Qiagen) including the optional DNaseI on-column digestion. RNA was quantified by Nanodrop and sent to the Duke Genomic Sequencing Core. RNA libraries for RNA-seq were prepared by the Duke Genomic Sequencing Core.