MiRNA signatures associated with lung involvement in systemic sclerosis (Small RNA-Seq)
MiRNA signatures associated with lung involvement in systemic sclerosis (Small RNA-Seq)
Plasma-derived small Extracellular Vesicles were isolated using standard ultracentrifugation protocol. Total RNA was extracted with the miRNeasy kit (Qiagen) following the manufacturerâ€™s protocol. Small RNA libraries (miRNA-seq) were prepared for sequencing using QIAseq miRNA Library Kit from Illumina