miRNA-seq of sheep hypothalamus
Small RNA sequencing of hypothalamic tissue from ovariectomized estradiol-treated Sunite sheep under short-to-long photoperiod transition (8L:16D for 42 days, then 16L:8D for 42 days, sampled at day 84). Libraries were constructed using NEB Next Ultra Directional RNA Library Prep Kit and sequenced on Illumina HiSeq 4000 (150 bp single-end).