16S rRNA sequencing for gastric cancer tissue
Illumina 16S Metagenomic Sequencing Library protocol to amplify the V3 and V4 regions (519-806R). The barcoded fusion primer sequences used for polymerase chain reaction (PCR) amplifications The final purified product was quantified using quantitative PCR employing the KAPA Library Quantification kit for Illumina Sequencing platforms according to the manufacturers protocol, and its quality was assessed using the LabChip GX HT DNA High Sensitivity Kit. Paired-end (2  300 bp) sequencing was performed using the MiSeq platform.