DNA-Seq of oral saliva: Baseline
DNA was extracted from stimulated saliva (n=120) and supragingival plaque samples (n=120)  using the ZymoBIOMICS 96 DNA kit. Metagenomics libraries were prepared with the Nextera XT DNA Library Preparation Kit. The HSD5000 kit in the TapeStation 4200 equipment was used for library quantification. The libraries were sequenced on a NovaSeq 6000 sequencing platform run in 150 paired-end mode.