GSM9029125: larva_head_10dpf_WT_rep2; Danio rerio; RNA-Seq
Total RNA was extracted from zebrafish heads homogenized in Trizol (Thermo Fisher Scientific) according to manufacturer's instructions. Total RNA samples were evaluated for quality using RIN scores generated from an Agilent Bioanalyzer 2100. RNA quantity was determined with Qubit fluorometer. The Illumina Stranded mRNA Library Preparation Kit was used to prepare sequencing libraries from 100 ng of high-quality RNA samples (RIN>9.5). The Kit procedure was performed without modifications. This procedure includes mRNA purification and fragmentation, cDNA synthesis, 3' end adenylation, Illumina adapter ligation, library PCR amplification and validation.