Halisarca dujardinii genomic DNA, PE150 Illumina dataset
Sponge tissue was homogenized in Eppendorf plastic tubes (1.5ml) with polypropylene pestle. Genomic DNA was extracted using a DNA isolation protocol suitable for both long-read and short-read sequencing.  Specifically, highly pure genomic DNA extraction was performed with the NucleoSpin Tissue kit (Clontech Takara), according to the manufacturers instructions. Genomic DNA concentration was quantified using a Qubit 3.0 fluorometer with Quantifluor dsDNA system (Promega). Next, gDNA was fragmented using Covaris s220 (USA) DNA shearing protocol. The length of DNA fragments was estimated using Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). Libraries for Illumina sequencing were prepared using NEBNext Ultra II DNA Library Prep Kit following the manufacturer's protocol. The concentration of libraries was measured by the Qubit 3.0 fluorometer. The quality and length of libraries was measured on Agilent 2100 Bioanalyzer using DNA High Sensitivity chip (Agilent  Technologies, Santa Clara, CA). The libraries were sequenced on the Illumina HiSeq 2500 instrument 151+151 bp mode