Sequencing of fecal cow metagenome: Diarrheic, Bishoftu
RNA was extracted from fecal samples using a combination of Trizol and GeneJet RNA extraction columns. RNA was pooled, DNAse treated and rRNA removed with the Ribo-Zero Plus rRNA depletion kit. Random amplification was performed using the the Ovation RNA-Seq System V2 kit. Amplified pools were library prepped using SMARTer ThruPLEX DNA-seq kit  and sequences on the NovaSeq 6000 system.