GSM9764339: Sglt2Cre_DOCA,rep1; Mus musculus; RNA-Seq
RNA was isolated from mouse kidney samples from Studies 2 and 3 using phenol: chloroform extraction and PureLink RNA Mini Kit (Invitrogen) per the manufacturer's instructions. RNA quantity (Qubit BR RNA Assay Kit; Invitrogen) and quality measures (RIN >8) (QIAxcel Advanced Bioanalyzer; Qiagen) were validated to confirm intact 18S/28S ribosomal RNA. Samples were then prepared using the TruSeq Stranded mRNA Library Prep Kit (Illumina) per the manufacturer's instructions. Samples were pooled into a single library and sequenced on the Illumina NextSeq 2000 platform using NextSeq™ 2000 P3 Reagent Kit (200 Cycles)