Genome sequencing of 8 Synechococcus isolates isolated from Martha's Vineyard Coastal Observatory
The long-read sequencing libraries were prepared using the SQK-LSK108 1D genomic DNA ligation kit (Oxford Nanopore, UK) with slight modifications (available at https://doi.org/10.17504/protocols.io.bixskfne) and sequenced on a MinION instrument using an R10.3 flow cell (Oxford Nanopore). FAST5 sequences were base called with Guppy v.2.3.1 (Oxford Nanopore Technologies) using flipflop mode. Adapters and split reads with adapters in the middle were removed using Porechop (https://github.com/rrwick/Porechop) with the flags require_two_barcodes and discard_middle.