RNA-seq of zebrafish kidney marrow
Total RNA from the KM cells was extracted from 12-month-old WT, SRSF2P95H or NRASG12D single and double mutant zebrafish for RNA-seq. Reads passing quality control were mapped onto the GRCz11 zebrafish reference genome with Ensembl gene annotation gtf (release 107). Differential gene expression (DEG) analysis was implemented by DESeq2 with default parameters.