24C018998 (CRX1958195)
Full-length HPV genomes of 41 types were used to design the HPV probes by MyGenostics (MyGenostics, Baltimore, MD, USA). Sequencing libraries were constructed following the instructions from Illumina. Genomic DNA was sheared to around 150-200 bp DNA fragments using a Covaris E-210 ultrasonicator (Covaris, Inc., Woburn, MA). These fragments were purified, end blunted, A-tailed and adaptor-ligated. The concentration of libraries was quantified by Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). The hybridization process was carried according to MyGenostics GenCap Target Enrichment Protocol (GenCap Enrichment, MyGenostics, USA). Libraries were hybridized with HPV probes including 41 types of HPV at 65 C for 24 h and then washed to remove uncaptured fragments. The eluted fragments were amplified by 18 cycles of PCR to generate libraries for sequencing. Libraries were quantified and proceeded to 101 cycles of paired-end index sequencing in the Illumina HiSeq 2000 sequencer according to manufacturer's instructions.