Pooled nasal swabs (n=46) from Copley Square, Boston, Massachusetts
Preprocessing: wet collect in HBSS, 2-step centrifugation at 1,200 x g & 6,000 x g, filter and concentrate with CP; any storage is without dissociation; Enrichment: includes DNase treatment (4ul 30 min); Extraction: includes QIAamp viral RNA Mini Kit extraction using vacuum manifold; Library prep: includes double-stranded cDNA synthesis (with RLB-modified random & TSO primers, and LunaScript RT), barcode PCR, and adapter ligation