GSM8516734: SAC_cKO_1; Mus musculus; OTHER
RNA was extracted using TRIzol (Qiagen), and mRNA was isolated using the Dynabeads™ mRNA DIRECT™ Purification Kit (Invitrogen). 50 ng of mRNA was used for sequencing library construction. RNA libraries for m6A-SAC-Seq were prepared using a manual protocol. After the treatment protocol described above, cDNA was synthesized using HIV Reverse Transcriptase (Worthington) and Actinomycin D (Sigma). cDNA libraries were then prepared using NEBNext Ultra II Q5 Master Mix and NEBNext Multiplex Oligos for Illumina (NEB).