May hypoxia replicate 4
After acclimation to 8C in an incubator, the single copepod was sealed in a 500-ul well of a microrespirometer plate containing filtered seawater. Dissolved oxygen was monitored in an incubator at 8C, and the individual was transferred to Trizol after the oxygen reached 1.16 mg O2 l-1. Total RNA was isolated with Zymo Directzol, and library prepared with Lexogen 3 Quantseq FWD kit.