RNAseq_Zibrowius_primnoidus
Twelve polyps were preserved in RNA later, washed in PBS, and processed using a TRIzol-based RNA extraction with magnetic beads. After purification (Mag-Bind Total Pure NGS kit), RNA integrity was assessed via TapeStation (High Sensitivity RNA Screen Tape Assay).Library preparation used the Zymo-Seq RiboFree Total RNA Kit for rRNA depletion, followed by paired-end 150 bp sequencing on an Illumina NovaSeq 6000, generating 90,741,564 raw reads.Primary bioinformatics included base calling, demultiplexing, and adapter masking with Illumina BCL Convert v3.9.31. The raw reads were trimmed using Trimmomatic v0.39, embedded within the Trinity assembler in RF mode, with the following parameters: ILLUMINACLIP:$TRIMMOMATIC_DIR/adapters/TruSeq3-PE-2.fa:2:30:10 SLIDINGWINDOW:4:5 LEADING:5 TRAILING:5 MINLEN:25