dGlul-1 (CRX2082030)
Total RNA was extracted from colonic crypts using the RNA-easy Isolation Reagent (Vazyme, R701-01). RNA quality and quantity were assessed using a NanoDrop spectrophotometer (Thermo Fisher Scientific) and Agilent 2100 Bioanalyzer (Agilent Technologies). Only RNA samples with an RNA Integrity Number (RIN) >7 were used for sequencing. RNA sequencing was performed on the Illumina NovaSeq 6000 platform (Illumina, San Diego, US) in PE150 mode.