GSM9437209: F1BM_21; Bos taurus; RNA-Seq
Samples were first disrupted and homogenized in TRIzol Reagent (Invitrogen™, Thermo Fisher Scientific, Waltham, MA, USA), and the aqueous phase was then processed using the RNeasy Plus Universal Mini Kit (Qiagen®, Germantown, MD, USA) according to the manufacturer's instructions. Sample integrity and purity were assessed using the Agilent 2100 Bioanalyzer and agarose gel electrophoresis. Sixteen samples (8 per treatment) at birth, 15 at the harvest of F1 heifers (LG n = 7; MG n = 8), and 16 samples (8 per treatment) from F2 fetuses met the quantity and quality parameters for library preparation. This process was carried out using the NEBNext® Ultra™ II non-directional RNA Library Prep Kit for Illumina (New England BioLabs®, Ipswich, MA, USA). The libraries were sequenced on the Illumina® NovaSeq 6000 platform, generating 150 bp reads at 20 million per sample depth. Both library preparation and paired-end sequencing were conducted by Novogene Co. (Nanjing, China).