NSUN2 shRNA knock down or control shRNA in 2D skin culture under differentiation conditions, using primary neonatal keratinocytes. This RNA-seq data was generated from the same samples as the Ribo-seq data in order to establish translation efficiency effects of NSUN2 knock-down.
NSUN2 shRNA knock down or control shRNA in 2D skin culture under differentiation conditions, using primary neonatal keratinocytes. This RNA-seq data was generated from the same samples as the Ribo-seq data in order to establish translation efficiency effects of NSUN2 knock-down. Total RNA was extracted using the RNeasy Plus kit (QIAGEN, 74136), followed by poly(A) enrichment and library preparation using the NEB Ultra II (non-directional) kit (E6111).