GSM9176753: Naïve YFP-2; Mus musculus; RNA-Seq
Then, RNA was extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions Libraries were prepared using 200 ng of total RNA and the Illumina TruSeq Stranded Total RNA library preparation kit. Libraries' qualities were evaluated using the PerkinElmer Labchip GX and qPCR using the Kapa Library Quantification Kit and the Life Technologies Viia7 Real-time PCR instrument. Libraries at a concentration of 2nM were sequenced on the Illumina HiSeq2500 using the High Output v4 chemistry. Raw FASTQ sequencing reads were mapped against the mm10 reference genome using the DRAGEN genome pipeline.