GSM9688195: Sample 10 KO; Mus musculus; RNA-Seq
Single nuclei were isolated from tissue samples using Miltenyi Nuclei extraction buffer (Miltenyi Biotec, Bergisch Gladbach, Germany) and resuspended in PBS + 1% BSA containing 0.4U/uL of RNase Inhibitor (NEB). Quality and quantity of nuclei was assessed by Acridine Orange and propidium iodide dye on a Cellometer Auto 2000 (Nexcelom). 30,000 nuclei were loaded on a 10x Chromium Controller using 10X Genomics 3' GEX v3.1 Platform (10x Genomics). Libraries were prepared by using 10x Chromium Next GEM Single Cell 3' GEM v4 kit.