Gene expression profile of NAFLD murine models (Part1 - NGS)
Gene expression profile of NAFLD murine models (Part1 - NGS)
Murine tissues for protein or RNA extraction were frozen at the time of collection and stored at -80. Since the murine data are derived from multiple partners, different RNA extraction methods have been used (miRNAeasy Mini Kits; Qiagen, RNeasy, Trizol; Qiagen, STAT-60, Trizol reagent; thermofisher, LifeTech Magmax-96) according to the manufacturer's instructions. All reagents, plastic ware, and supplies used were nuclease-free, sterile, and of molecular biology grade. RNA Integrity Number (RIN) of 8 was considered the lowest cut-off for RNA sequencing. RNA from tissues (1 ug RNA) were used to generate barcoded sequencing libraries using Illumina TruSeq® Stranded mRNA Library Preparation Kit (Illumina) following manufacturer's instructions.