ITS of tobacco-wheat rotation soil
Amplification and sequencing of the fungal ITS1 region. The primer pair ITS1F (5'-CTTGGTCATTTAGAGGAAGTAA-3') and ITS2 (5'-GCTGCGTTCTTCATCGATGC-3') was used for PCR amplification. Sequencing was performed on the Illumina NextSeq 2000 platform with a paired-end (2x300 bp) strategy.