GSM9616113: Polymicrobial bEVs, Replicate 3; Streptococcus sanguinis SK36; Staphylococcus aureus subsp. aureus str. Newman; Prevotella melaninogenica ATCC 25845; Pseudomonas aeruginosa PA14; ncRNA-Seq
RNA was isolated from bEVs using the miRNeasy Kit (Qiagen) with lysozyme (800 µg/mL) and lysostaphin (12.5 µg/mL) to lyse S. aureus vesicles. 100 ng of RNA was used as input for library preparation. RNA libraries the NEBNext Low-bias Small RNA Library Prep Kit (New England Bio Labs, Cat. No. E3420L) according to the manufacturer's instructions.