Mesocentrotus nudus
The genomic DNA was sheared into fragments with length ~350 bp using S220 Focused-ultrasonicators (Covaris, USA). Adapters were ligated onto the 3 end of the sheared fragments. After PCR amplification and purification, the final libraries were sequenced on the Illumina Novaseq platform (Illumina Inc., USA) and 150 bp paired-end reads were generated.