GSM9001746: snATAC-seq in chALL1 and iALL3 KMT2A::AFF1 blasts; Homo sapiens; ATAC-seq
Cryopreserved bone marrow cells were thawed, and 16,000 live CD19+ blasts of KMT2A-AFF1+ ALL patient samples were FACS-sorted and nuclei were extracted using the recommended protocol (Chromium). Male/female sample pairs were loaded together on one Chromium 10x lane for processing with the Single Cell Multiome ATAC + Gene Expression protocol. Sequencing was performed on a NovaSeq 6000, with gene expression libraries sequenced on a PE150 S4 flow cell and ATAC-seq libraries on an SP PE50 flow cell. 10x scMultiome