DNA-seq:ec174
Genomic DNA was extracted from E. coli isolates using the Qiagen DNeasy Blood & Tissue Kit, followed by quality assessment with NanoDrop and Qubit. Library preparation was performed using the Illumina Nextera XT DNA Library Prep Kit, involving tagmentation, indexing PCR, and AMPure XP bead purification. The libraries were quantified using Qubit and analyzed with an Agilent Bioanalyzer for fragment size distribution. Normalized libraries were pooled and sequenced on an Illumina MiSeq platform using a 600-cycle v3 kit for paired-end 2  300 bp reads. Raw sequences were processed with FastQC and trimmed for quality before downstream analysis.