GSM9792570: Newborn Distal, replicate 2, RNA_seq; Mus musculus; ATAC-seq
Colonic epithelial cells were isolated from mouse tissues and processed immediately for ATAC-seq library preparation. Cells were washed with ice-cold PBS and lysed in a detergent-containing lysis buffer to release intact nuclei. Nuclei were collected by centrifugation, washed, and resuspended in transposition reaction buffer for subsequent tagmentation. ATAC-seq libraries were prepared using the Novoprotein ATAC Library Prep Kit (Novoprotein, China) according to the manufacturer's instructions. Briefly, purified nuclei were incubated with Tn5 transposase at 37°C for 30 min to simultaneously fragment accessible chromatin and insert sequencing adapters. Following transposition, DNA was purified using magnetic beads and amplified by PCR with indexed primers. Amplified libraries were purified, quantified using Qubit fluorometry, and assessed for fragment size distribution using an Agilent Bioanalyzer. Libraries were sequenced on an Illumina platform with paired-end 150-bp reads (PE150).