1855347_EPI2754
Frozen stool samples were homogenized in PBS (20% w/v) and filtered through 0.45um spin columns. Fecal filtrate was incubated for 30 minutes at 37 C with a nuclease cocktail composed of TURBO DNase (2U/ul), Baseline-ZERO DNase (1U/ul), Benzonase (25U/ul), and RNase A (0.5U/ul). Total nucleic acid was extracted from the samples with MagMAX Total Nucleic Acid Isolation Kit (Life Technologies, AM1840) on ThermoFisher Scientific KingFisher Flex Magnetic Particle Processor. Samples were amplified using a random-primer protocol.