One way or another: Differential impact of pharmacologic inhibitors on extracellular vesicles derived from resting neutrophils
One way or another: Differential impact of pharmacologic inhibitors on extracellular vesicles derived from resting neutrophils
For RNA sequencing, EVs were isolated from three biological replicates. The dry EV pellets were resuspended in 10 μL sterile distilled water (nuclease-free), snap frozen, and stored at -80°C until processing within 1 week. Total RNA was extracted using a Qiagen RNA extraction kit, following the manufacturer's instruction Poly(A)+ RNA libraries were prepared using the SMARTer Stranded V2 kit.