GSM8581435: Ex vivo, E4.5 embryo; Mus musculus; RNA-Seq
E4.5 in vivo embryos and 24h-cultured ex vivo embryos were microscopically isolated, immediately soaked in Accumax (Nacalai Tesque, Japan) at room temperature, and dissociated with glass capillaries. The capillaries were sequentially changed from thicker to thinner ones, and only dispersed single cells were manually picked up into 0.04% BSA (wt/vol) in PBS(-). The single-cell suspension from a total of 33 in vivo embryos at E4.5 from 4 mice and 32 ex vivo embryos at 24h from 4 donor mice was immediately processed for RNA sequencing library preparation using Chromium Next GEM Single Cell 3' Library and Gel Bead Kit v3.1 (PN-1000268, 10x genomics, USA), Chromium Next GEM Chip G Single Cell Kit　(PN-1000120, 10x genomics, USA), and Chromium iX (10x genomics, USA).