GSM8581856: IF_2; Mus musculus; RNA-Seq
Rna was isolated with PeqGOLD total RNA kit (Peqlab); quality was checked with Tape-Station (RNA screen tape, avg RIN:9) BGI Strand-Specific mRNA Library Preparation (DNBSEQ); Single-stranded circle DNA molecules are replicated via rolling cycle amplification, and a DNA nanoball (DNB) which contain multiple copies of DNA is generated. Sufficient quality DNBs are then loaded into patterned nanoarrays using high-intensity DNA nanochip technique and sequenced through combinatorial Probe-Anchor Synthesis (cPAS)