16S rRDA sequencing of stool and intestinal contents of Mus musculus
16S rRNA sequencing was performed at the University of Chicago Duchossois Family Institute in the Microbiome Metagenomics Facility (DFI MMF). DNA extraction from intestinal contents was performed using the QiaAmpPowerFecal Pro DNA (Qiagen, cat. # 51804) and the V4-V5 region of 16S rRNA- genes PCR amplified using barcoded dual-index primers (520FWD- AYTGGGYDTAAAGNG and 909REV-CCGTCAATTYHTTTRAGT). Illumina compatible libraries were generated using the Qiagen QIASeq 1-step amplicon kit (Qiagen, cat. #180419 and sequencing performed on an Illumina MiSeq platform using a 2~250 Paired-End reads, generating >10,000 reads per sample.