GSM8581497: wounding 4H, rep3; Oryza sativa; RNA-Seq
Total RNAs were extracted from rice leaves samples using the RNeasy Plant mini kit (Qiagen) with addition of an on-column DNase I digestion. For each sample, 500 ng of the extracted total RNA was used for library construction. Libraries were constructed using Truseq RNA Sample Preparation Kit V2 (Illumina, CA, USA), following the manufacturer's instructions. Adapters with index sequences were attached to the libraries. After the average length of the libraries was confirmed, the concentration of each library was adjusted to 10 nM.