GSM9111627: WT-LPS2-INPUT; Mus musculus; OTHER
Total RNA was extracted and purified with TRIzol reagent (Invitrogen, USA) following the manufacturer's procedure. The RNA amount and purity were examined using a Nanodrop 2000 (Thermo Fisher Scientific, USA). After total RNA was extracted, the enriched RNA was fragmented into short fragments (about 100nt) by using fragmentation buffer. RNA was divided into two parts, one of which was used as the input (no IP experiment was performed). The other RNA was enriched with m6A specific antibody. The enriched RNA was reverse transcripted into cDNA with random primers. Next, the cDNA fragments was end repaired, and ligated to Illumina sequencing adapters. Finally we got qualified library for sequencing, sequenced using Illumina HiSeqTM 4000 (or other platforms) by Gene Denovo Biotechnology Co. (Guangzhou, China)