GSM8580008: G6/7R TNF-NTF CART, +THP1, donor 3; Homo sapiens; RNA-Seq
The CD8+ CAR-T cell populaiton was isolated by an MA900 Cell Sorter (SONY) on day 10. Total RNA was isolated from the purified CD8+ CAR-T cells using the Nucleospin RNA Kit (Macherey-Nagel) according to the manufacturer's instructions. Library preparation was conducted by NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB)