GSM9796530: Ovis aries, plasma, CTRL, week 14, replicate 1; Ovis aries; miRNA-Seq
Total RNA including small RNA was extracted from 200 µL plasma using the QIAseq miRNA Library Kit (QIAGEN). RNA quality and quantity were assessed by Qubit fluorometry and TapeStation analysis. Small RNA libraries were prepared using QIAseq miRNA Library Kit (QIAGEN) with UMI-based deduplication. Libraries were sequenced on the AVITI platform (Element Biosciences) using 1x76 single-end reads.