Short-read whole-genome sequencing of isolates collected from the study assessing cross-generational interactions between the host and the gut pathogen, Pseudomonas entomophila, in Drosophila melanogaster.
Samples were prepared for whole genome sequencing using the Illumina DNA Prep tagmentation kit and IDT For Illumina Unique Dual Indexes. Sequencing was performed on the Illumina NextSeq2000 platform using a 300-cycle flow cell kit to produce 2x150bp paired reads. 1-2% PhiX control was spiked into the run to support optimal base calling. Read demultiplexing, read trimming, and run analytics were performed using DRAGEN v3.10.12, an on-board analysis software on the NextSeq2000.