GSM9107633: acites_lavage_samples_HTO; Mus musculus; OTHER
Cell suspensions from omentums were prepared by mechanical dissociation, followed by enyzmatic digestion using collagenase and DNAse I. The single cell suspension was then sorted for ZombieRed negative live cells. For ascital fluid, RBC lysis was performed along with several 1xPBS washes. Samples were subjected to FcR block, followed by HTO staining. 3x libraries from each lane are sequenced separately on a NovaSeq X machine. Transcriptome library preparation was conducted according to the Chromium Next GEM Single Cell 3' Reagents Kits v3.1 with Feature Barcoding technology kit manual. Hashtag libraries were prepared using the standard CITE-seq protocol (https://citeseq.files.wordpress.com/2019/02/cite- seq_and_hashing_protocol_190213.pdf). LARRY barcode libraries were constructed using a custom nested PCR protocol followed by indexing.