GSM9081041: RiboTag, AAV-shControl (Input) 1; Mus musculus; RNA-Seq
For RiboTag sequencing, lysate from the freshly collected DRGs were immunoprecipitated with an anti-HA antibody, and the RNA from the immunoprecipitated product was extracted using RNeasy mini kit(Qiagen, 74104). Libraries were prepared following the instrcutions from the manufacture. (cDNA-PCR barcoding kit(SQK-PCB112.24))