A functional crosstalk between the BMP-SMAD pathway inhibitors TMPRSS6 and FKBP12 regulates hepcidin expression in vivo
A functional crosstalk between the BMP-SMAD pathway inhibitors TMPRSS6 and FKBP12 regulates hepcidin expression in vivo
Two days after the last injection mice were sacrificed and liver was extracted for molecular analysis C57BL/6 mice (eight-week-old) were purchased from Charles River and were treated twice a week for six weeks with triantennary N-acetyl galactosamine (GalNAc)-conjugated antisense oligonucleotide (ASO) against Fkpk12 or scramble (used as control), in a dose of 5 microgram per gram body weight. Total RNA was extracted from liver using TRIFAST (Euroclone) according to manufacturerâ€™s instruction. mRNA-Seq libraries were created according to the standard Illumina TruSeq protocol.