Metagenomic sequencing of biofilm from rocks in the intertidal region by the outflow from the Rothera sewage treatment plant
DNA was extracted from biofilm using the DNeasy Power Biofilm Kit (Qiagen). Purified on AMPure XP beads. 400ng of DNA was used in the Rapid Barcoding Kit (Oxford Nanopore) and sequenced on a MinION flowcell R9.4.1