GSM9064286: INTS11 ChIP-seq, 0h, rep1; Homo sapiens; ChIP-Seq
Cells were crosslinked by incubating with standard growth media supplemented with 1% formaldeyde for 10 min. Crosslinking was quenched by adding glycine to a concentration of 125 mM. Cells were centrifuged and sonicated to generate cell lysate and shear DNA. Sheared DNA samples were incubated overnight with 5 μg of INTS11 or control IgG antibody for the immunoprecipitation. The resulting DNA was extracted using phenol/chloroform/isoamyl alcohol. The libraries were generated using the Takara ThruPLEX Tag-seq Kit according the manufacturer protocols.