Illisha_elongata_HiC_muscle
Cell Preparation: Fix cells or tissues to preserve the spatial organization of chromosomes.Nuclei Isolation: Isolate nuclei and digest the cell membrane.Restriction Digestion: Fragment chromatin using a restriction enzyme like MboI.End Repair and Adapter Ligation: Repair sticky ends and ligate biotinylated adapters.Proximity Ligation: Incubate chromatin to ligate ends in close proximity.Reverse Crosslinking: Release DNA from chromatin through heat treatment.Biotin Pull-down: Selectively capture biotinylated DNA fragments using streptavidin beads.PCR Amplification: Amplify the captured DNA fragments.Size Selection: Select the desired size range of DNA fragments.Sequencing: Sequence the Hi-C library using a high-throughput platform.