GSM8766559: Naive_IVpos_res_cDC2_3; Mus musculus; RNA-Seq
500 of each DC cell type was sorted using the BD Aria II cell sorter directly into reaction buffer from the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara), and reverse transcription was performed followed by PCR amplification to generate full-length amplified cDNA. Libraries were sequenced by the Genomics Core at Benaroya Research Institute. Quality of RNA was determined on the Agilent Tapestation. Sequencing libraries were constructed using the NexteraXT DNA library preparation kit (Illumina) and samples were run on an Illumina NextSeq2000 to generate paired-end 59-base reads with a depth of 5 million reads per sample.