WGS of Rhopalodia californica 22EEL08 culture
Cells were pelleted and resuspended in 900 L TE and 100 L of 1% Triton-X (in TE) to wash away the majority of exogenous bacteria before DNA extraction. DNA was extracted using the Qiagen DNeasy PowerBiofilm kit according to the manufacturer's instructions. Metagenomic libraries were prepared using the Illumina DNA Prep tagmentation kit. Paired-end short read sequencing was performed by 150 cycles of sequencing on an Illumina NextSeq2000 platform.