GSM9438758: Lung RNA-seq, NC replicate 1; Mus musculus; RNA-Seq
Total RNA was extracted from mouse lung tissues using TRIzol reagent according to the manufacturer's instructions. RNA integrity/quality was assessed (e.g., Agilent TapeStation RNA ScreenTape), and only high-quality RNA was used for library construction. Libraries were prepared using the TruSeq Stranded Total RNA Library Prep Gold Kit (Illumina) following the TruSeq Stranded Total RNA Reference Guide (1000000040499 v00). Sequencing was performed on an Illumina platform using paired-end reads (read length 101 bp).