GSM8864260: mCherry cells, early-HSPCs_6 dpf; Danio rerio; RNA-Seq
Zebrafish larvae were minced with a razor blade and collected into PBS. The suspension was then digested with Liberase for 15 min at 37C. FBS was used to inactivate the librase. Cells were filtered once before isolating via FACS. Libraries were prepared according to manufacturer's instructions of the kits. For experiments (1) lineage tracing & (2) runx1 mutants, the Chromium Next GEM Single Cell 3' Kit v3.1 was used. For experiment (3) slc24a5/rag1/R848, the Chromium Next GEM Single Cell 3' Kit v4 was used.