vernal pool COI metabarcoding (invertebrates, COI-fwgF2 and COI-EPTDr2n primers fro Elbrecht & Leese 2017)
library preparation included size-selection cleanup with Ampure XP beads (Beckman, Coulter), indexing PCR with Phusion HF premix (ThermoFisher) and Nextera indices (Illumina), pre-pooling DNA quantification with Qubit, pooling of samples in equimolar concentrations, library quality checks on a BioAnalyzer DNA100 chip (Agilent) and KAPA quantification kit (Roche), and a PhiX spike (Illumina) to balance fluorescence signals