HiFi long-reads of Arctic tundra soil
A total of 5 g of DNA was used to construct a high-fidelity (HiFi) sequencing library with the SMRTbell Prep Kit 3.0 (PacBio, CA, USA). No shearing was performed prior to library preparation. The resulting library was quantified using a Qubit fluorometer (Thermo Fisher Scientific, DE, USA) and analyzed on a Femto Pulse system (Agilent, CA, USA) to confirm library size for quality control. Sequencing was performed using single-molecule real-time (SMRT) sequencing on the PacBio Revio system at the MACROGEN Inc (Seoul, Korea) and TERAZEN Inc (Seongnam, Korea).