WGBS-TK5 (CRX1831264; CRA027613)
Genomic DNA is broken into 100-300 bp fragments by ultrasound. End repair of DNA fragments, addition of A base at the 3 'end, and connection of sequencing adapters. ZYMO EZ DNA Methylation Gold kit was used for Bisulfite treatment. After desalination treatment, the gel was cut and recovered, and the library fragment size was selected. After PCR amplification, the library fragment size was selected again. After the construction of the library is completed, perform quality testing on the library. The library that passes the quality inspection will be used for machine sequencing. submitted_instrument_model: Illumina HiSeq X Ten