GSM9064249: B-4N-p180-liver-sc; Mus musculus; RNA-Seq
10x Genomics® Cell Preparation Guide describes best practices and general protocols for washing, counting and concentrating cells from both abundant and limited cell suspensions (greater than or less than 100000 total cells, respectively) in preparation for use in 10x Genomics Single Cell Protocols. The cell suspension was loaded into Chromium microfluidic chips with 3' v3 chemistry and barcoded with a 10× Chromium Controller (10XGenomics). RNA from the barcoded cells was subsequently reverse-transcribed and sequencing libraries constructed with reagents from a Chromium Single Cell 3'  v3 reagent kit (10X Genomics) according to the manufacturer's instructions. Sequencing was performed with Illumina  NovaSeq6000  according to the manufacturer's instructions (Illumina).