16S amplicon sequencing of Fildes peninsula lake water
Sediment was collected using a shavel. Each sample corresponds to                 aprox. 350 mg liters of sediemto. DNA was extracted using the DNeasy Power Soil kit                 (Qiagen). The V4-V5 region of the 16S rRNA gene was amplified from 1:10 diluted DNA                 samples, using primers 515F and 928R (Wang & Qian, 2009) and Phusion Hot Start                 II High-Fidelity DNA Polymerase (Thermo Scientific), with annealing temperature of                 72C. PCR products were paired-end sequenced on the Illumina Miseq platform (2 x                 250-bp) (DNA Sequencing Facility, University of Wisconsin Biotechnology Center,                 Wisconsin, USA).