Nanopore adaptive whole genome sequencing of washed and unwashed Schistosoma mansoni miracidia
Whole genome amplification (WGA) was performed on single Schistosoma mansoni miracidia isolated from FTA cards, using the GenomiPhi V2 DNA amplification kit. Amplified DNA was cleaned with AMPure beads and quantified using Qubit, Nanodrop, and TapeStation. Libraries were prepared with the Oxford Nanopore ligation kit (SQK-LSK114) and sequenced on MinION Mk1B devices using FLO-MIN114 flowcells. One sample was sequenced per flowcell without barcoding, and adaptive sampling was applied using the S. mansoni reference genome version 10 for enrichment. Flow cells were washed and reused for second library runs to maximise data yield.