RNA-Seq of rattus norvegicus: adult male pbmcs
For library preparation, a total of 500ng of initial RNA was required, and the Illumina Stranded mRNA Prep kit (20040534) was used following manufacturers instructions. The resulting DNA libraries, containing retrotranscribed and modified RNA fragments, were quantified using microfluidic electrophoresis on Agilents Tape Station instrument and the Agilent DNA High sensitivity Screen Tape Kit.