RNA-Seq of Schistosoma mansoni miracidia hatched from liver-derived eggs
The NEBNext Ultra II kit was used to prepare sequencing libraries. 10 ng of total was used as input for the NEBNext Poly(A) mRNA Magnetic Isolation Module. dsDNA fragments with adapters were amplified via 17 PCR cycles and the library was purified with 0.8X NEBNext sample purification beads. Quantity and quality of libraries were assessed with a Qubit and 4200 Tapestation. All 8 libraries were barcoded, pooled, and sequenced on a full 10B lane of a NovaSeq X with a 2x150 format.