Metagenomic sequencing of Myotis austroriparius: frozen rectum tissue
Total RNA was DNase-treated and reverse transcribed using SuperScript IV with random hexamers and oligo(dT) primers, followed by second-strand synthesis and purification of double-stranded cDNA. Libraries were prepared using the NEBNext Ultra II FS DNA Library Prep Kit with dual indexing, bead-purified, quality-checked, size-selected (200500 bp), and quantified with a Qubit Fluorometer.