Adding 5 nematodes per gram of dry soil to the petroleum-contaminated soil with a water content of 50% and a temperature of 22C at Site 1
The primer pair used to amplify the V3-V4 hypervariable regions of the bacterial 16S rRNA gene was 341F(CCTACGGGNGGCWGCAG) 814R(GGACTACHVGGGTATCTAAT)