Whole Genome Illumination Sequencing of P. tricornutum CCAP 1055/1
Genome-wide sequencing was performed on P. tricornutum CCAP 1055/1 to determine the locations of single-nucleotide polymorphisms (SNPs) present in the strain to inform sgRNA guide design. Genomic DNA sequencing libraries were prepared with NEBNext Ultra II FS DNA Library Prep Kit (NEB, Cat.# E6177S) using the manufacturer protocol for inputs >= 100ng. The library was sequenced on an Illumina MiSeq v3 flow cell (2  150 bp).