RNA-seq of PDAC cell treated with AMG510
Agilent 2100 Bioanalyzer (ABI) was performed to assessed quantity and quality of the cDNA libraries. The Double-stranded PCR products were treated with thermal denaturation and cyclization reaction using the splint oligo sequence and the final library was generated. Phi29 (Thermo Fisher) was utilized for amplifying the single-stranded circular DNA and then a DNA nanoball was generated.