GSM9761848: Capsid Anchor pGFP Transfected cells (1); Homo sapiens; RNA-Seq
Total RNA was extracted from the received human cells samples using TRIzol method followed by column purification using Quick RNA Mini Plus kit (Zymo Research) as per manufacturer's instruction. Quality and quantity of the extracted RNA samples were checked on NanoDrop followed by Agilent Tape station using High Sensitivity RNA ScreenTape. The sequenced raw data of 4 samples were processed to obtain high quality clean reads using Trimmomatic v0.39 to remove adapter sequences, ambiguous reads (reads with unknown nucleotides “N” larger than 5%), and low-quality sequences (reads with more than 10% quality threshold (QV) < 25 phred score). A minimum length of 100 nt (nucleotide) after trimming was applied. After removing the adapter and low-quality sequences from the raw data, high quality reads were obtained. These high quality (QV>25), paired-end reads were used for reference based read mapping.