NanoSeq of homo sapiens: normal human urothelium exposed to BKPyV
Ureteric urothelial cells were isolated from excess tissue after renal transplant. Cells were expanded then underwent biomimetic re-differentiation, forming a tight barrier. Cells were then exposed to BKPyV and maintained until 21 days post infection when DNA was harvested. DNA was then processed according to the Sanger Institute (Cambridge, UK) NanoSeq pipeline.