GSM9104871: T_A1; Rattus norvegicus; OTHER
DNA was isolated by grinding, shaking, centrifugation, adsorption, elution with Mixed lysis buffer (buffer) containing Proteinase K, Isopropyl alcohol buffer solution, Ethanol buffer solution, ddH2O and so on. Microbial DNA was extracted from fecal samples, and the 16S rRNA gene region was amplified using specific primers. The amplified DNA fragments were used to construct sequencing libraries. High-throughput sequencing, typically performed using the Illumina platform, was conducted to generate sequence data.