GSM8765868: EAE_5ADB.Scramble_Astrocytes_4; Mus musculus; RNA-Seq
Sorted cells were lysed and RNA isolated using the Qiagen RNeasyMicro kit (Qiagen, 74004) with on-column DNase I digestion (Qia-gen, 79254). For the transcriptome of Acly+Ep300+ astrocyte subsets was isolated and sequenced using the FIND-seq/Smart-seq3 protocol. The DNA library was prepared using the Nextera XT Library Prep Kit (Illumina, FC-131-1024), following the manufacturer's protocol.