Multi-omics profiling of jejunum and duodenum in Longyan Shan Partridge ducks with divergent residual feed intake KN_20
Total genomic DNA was extracted from the samples using the E.Z.N.A. Soil DNA Kit. The quality, concentration, and purity of the extracted DNA were assessed via agarose gel electrophoresis and a NanoDrop2000 spectrophotometer. The V3-V4 hypervariable region of the 16S rRNA gene was amplified via PCR using the 338F/806R primer set. The PCR products were purified, and sequencing libraries were constructed using the NEXTFLEX Rapid DNA-Seq Kit. Sequencing was performed on an Illumina Nextseq2000 platform.