mtDNA-seq of seawater metagenome: seawater from Indian Harbour
Environmental DNA (eDNA) was extracted from surface seawater samples collected at multiple Arctic sites to assess biodiversity and community structure in marine ecosystems. Samples were collected in quadruplicate at each site using a Niskin bottle at a depth of 4 meters. DNA was extracted using the DNeasy PowerWater Kit (Qiagen) and sequenced using the Illumina MiSeq platform for metabarcoding analysis targeting the COI gene region. The goal of this study is to monitor spatial variation in Arctic macro-organismal communities and to establish a comprehensive portrait of Arctic biodiversity using eDNA metabarcoding.