RNA-seq of earthworm-gut inhabiting natural farming field
Metagenomic data was extracted from earthworm gut using Nucleospin DNA kit. Quality of DNA Sample was analysed using Nanodrop by determining A260/280 ratio. Amplicon library was prepared using Nextera Index Kit (Illumina inc.) as per the 16S metagenomic sequencing protocol. Bacterial specific region primers are used and amplicon libraries obtained were purified and analysed on 4200 Tape station system.after obtaining mean peak size, libraries were loaded onto Miseq at 10pM for cluster generation and sequencing.