GSM8489210: cmt3-11 mutant in Col-0; Arabidopsis thaliana; Bisulfite-Seq
DNA was extracted using conventional CTAB method. For bisulfite sequencing, the genomic DNA was fragmented to a mean size of 100-300 bp by sonication using a Covaris S220 focused-ultrasonicator, followed by end-repair, 3'-end adenylation and methylated adaptor ligation using Illumina TruSeq DNA kit. Then, bisulfite conversion was performed using a Zymo EZ DNA Methylation-Lightning kit, and the bisulfite-converted, adaptor-ligated DNA was enriched by PCR for 12 cycles using KAPA HiFi HotStart Uracil+ Kit, purified with Agencourt beads and quantified by Qubit HS dsDNA kit.