snRNA-seq of Mus musculus: adult female heart tissue
Single-nucleus suspensions were isolated from frozen mouse heart tissue. Cardiac tissue was mechanically homogenized and filtered to obtain intact nuclei. Nuclei were purified and counted before library preparation. Single-nucleus RNA-seq libraries were generated using the 10x Genomics Chromium Single Cell 3 Reagent Kit (v3.1) according to the manufacturer's instructions. Approximately 5,00010,000 nuclei were loaded per sample. cDNA libraries were constructed following reverse transcription, amplification, and fragmentation. Sequencing was performed on an Illumina NovaSeq 6000 platform to generate paired-end reads.