GSM9687137: scRNA-seq, Spleen, Uninfected, R848-Treated; Mus musculus; RNA-Seq
Spleens were isolated and minced to obtain single cell suspension. Samples were treated with RBC lysis buffer then filterd though a 70um filter. The spleen cells from three separate mice were combined into one sample then counts and analyzed for cell death. Single-cell 5' cDNA gene expression libraries were generated using the 10X Genomic Chromium Single-Cell 5' Library and Gel Bead Kit v1 and 10x Chromium Controller (10x Genomics, Pleasanton, CA) platform for microdroplet-based, single-cell barcoding. Sequencing ws performed on the NovaSeq Sequencing System.