GSM9110934: iPSC-derived pericytes 4; Homo sapiens; RNA-Seq
RNA was extracted from cell pellets using Trizol (Qiagen, Cat# 79306) and purified using the RNeasy mini kit (Qiagen, Cat# 74106) following manufacturer's instructions. Total RNA concentration was determined using a NanoDrop spectrophotometer. RNA was stored at -80ºC. Library construction was done as previously described by BGI (Chen et al. 2018, SOAPnuke: a MapReduce acceleration-supported software for integrated quality control and preprocessing of high-throughput sequencing data) using 400ng of RNA of each cell type.