ATAC-seq: human bladder, male, 83 years
Snap frozen tissue was pulverized, resuspended in lysis buffer, and nuclei were filtered with a 30 micron filter. 50,000 nuclei were used for transposition. Transposition and fragmentation were performed by adding Tn5 transposase. Tagmented DNA was purified, ligated with adapters, and amplified before sequencing.