GSM9212097: female zebra fish, 200 μg/L DBAN_2, day 56; Danio rerio; RNA-Seq
The total RNA was extracted by TruSeq RNA sample Preparation kit V2, and mRNA was sorted. The mRNA with poly (A) structure was specifically bound by magnetic beads with oligo-dT. The purified mRNA was synthesized into cDNA under the action of reverse transcriptase and polymerized into double-stranded cDNA. The double-stranded cDNA end repair reaction was performed, and the 3 ' end plus ' A ' buffer reaction system was added. The illumina sequencing adaptor was connected to both ends of the DNA by T4 DNA ligase to construct an RNA library.