GSM8766414: 1 - WT CD  2; Mus musculus; RNA-Seq
Total RNA was extracted using the Quick-RNA MiniPrep Kit (Zymo Research, R1055). RNA quality and yield was assessed by NanoDrop ND1000 (Thermo Scientific, Waltham, MA), then reverse transcribed into cDNA with the iScript cDNA Synthesis Kit (Bio-Rad Laboratories, 1708891) Bulk RNA sequencing was performed at the Genome Analysis Core of Medical Genome Facility at Mayo Clinic, Rochester. mRNA sequencing libraries were prepared and sequenced on an Ilumina HiSeq 2000 instrument at the Mayo Clinic Center for Individualized Medicine Medical Genomics Facility.