GSM9830039: lib10_round1-R5; synthetic construct; SELEX
Nucleotide sequences bound to target proteins were eluted using 100 μL of ddH2O by heating at 95 ℃ for 10 min. Enriched ssDNA pools from each SELEX round were end-repaired, A-tailed, and ligated with sequencing adapters following standard Illumina library preparation protocols. Barcodes were incorporated for multiplexed sequencing. Libraries were amplified by limited-cycle PCR and purified to remove primer dimers and non-specific products before sequencing.