GSM9761459: WT-2; Mus musculus; ncRNA-Seq
pEVs were isolated from 200 µL plasma using the Total Exosome Isolation Kit (#4484450, Invitrogen, Vilnius, Lithuania) following manufacturer's protocol. EV RNA was extracted using the ExoQuick Exosome RNA Purification Column Kit (EQ808A, SBI, CA). Small ncRNA libraries were prepared using the NEBNext Multiplex Small RNA Library Prep Set (#E7560S, NEB, MA).