RNA-seq of maize: inoculated stalk
Strand-specific RNA sequencing and data analysis were conducted by Seqhealth Technology Co., LTD (Wuhan, China). Total RNA was extracted from the stalks using TRIzol Reagent (Invitrogen, 15596026, USA). RNA quality was assessed by examining the A260/A280 ratio with a NanodropTM OneC spectrophotometer (Thermo Fisher Scientific Inc). RNA Integrity was confirmed through 1.5% agarose gel electrophoresis. Qualified RNAs were quantified using Qubit3.0 with the QubitTM RNA Broad Range Assay kit (Life TechnologiesQ10210). Two micrograms of total RNA were utilized for stranded RNA sequencing library preparation, following the manufacturers instruction, using the KCTM Stranded mRNA Library Prep Kit for Illumina (DR08402, Wuhan Seqhealth Co., Ltd. China). PCR products corresponding to 200-500 bp were enriched, quantified and sequenced on the DNBSEQ-T7 sequencer (MGI Tech Co., Ltd., China) with the PE150 model.