GSM9688139: B16-F10, SSBP4 WT, Rep 2; Mus musculus; ATAC-seq
Cryopreserved cells were thawed and cell viability assessed using AO/PI dye; samples w/ ≥75% viability were used for downstream processing. Cells were collected and gently lyssed to oslate intact nuclei, which were then subjected to Tn5 transposase-mediated tagmentation. Samples were processed with the Active Motif ATAC-Seq Kit (Cat. No. 53150) according to manufacturer protocols.