GSM9437662: TG_Sham_female_rep3; Mus musculus; RNA-Seq
bilateral TGs were harvested, dissociated, and cell viability was assessed by microscopy. DiI⁺ neurons were sorted directly into 500 µL TRIzol reagent and stored at −80 °C until RNA extraction using TRIzol protocol. RNA extraction, library construction and sequencing were performed at the IRIC Genomics Platform (Université de Montréal). RNA integrity was confirmed with an Agilent Bioanalyzer. Poly(A)+ RNA was enriched from total RNA and sequencing libraries were prepared using the KAPA RNA HyperPrep Kit with Poly(A) selection following the manufacturer's instructions. Libraries were sequenced on an Illumina NextSeq 500 platform with single-end reads generated over 75 sequencing cycles (maximum read length 1×85 nt).