GSM9762062: Young Brain ECK4KO RNA polymerase ChIP seq replicate1; Mus musculus; ChIP-Seq
Brain endothelial chromatin was prepared from mouse brain tissue. Chromatin was crosslinked, fragmented, and immunoprecipitated using an antibody against RNA Polymerase II. DNA-protein complexes were captured, washed, reverse-crosslinked, and purified. Sequencing libraries were prepared from the recovered DNA and sequenced on an Illumina platform. For RNA Polymerase II ChIP-seq, chromatin isolated from purified brain endothelial cells was immunoprecipitated using antibodies against RNA Polymerase II, followed by sequencing library preparation according to standard ChIP-seq protocols.