GSM8691785: R_h2al2KO_rep1; Mus musculus; RNA-Seq
Spermatogenic cell fraction purification was performed as previously described (Shiota et al. 2018). For each sample, one µg of RNA (RIN = 10) was used for library preparation with TruSeq Stranded Total RNA (RiboZero Human/Mouse/Rat) Library Prep (Illumina, Cat#RS-122-2201) according to the manufacturer's instructions. Each library was quantified on Qubit with the Qubit® dsDNA HS Assay Kit (Life Technologies) and the size distribution was examined on the Fragment Analyzer with High Sensitivity NGS Fragment Analysis kit (Agilent). The libraries were then sequenced on the Illumina NextSeq 500 (paired-end 75) at the TGML Platform of Aix-Marseille University (France).