GSM8580376: LDS infection 1, 48hr; Mus musculus; RNA-Seq
Cells were treated with Trizol and kept at -80ºC until frozen. RNA isolation was done by using Thermo Fisher Invitrogen™ PureLink™ Plant RNA Reagent, as per manufacturer's protocols, followed by quantification was done by using Qubit BR Assay kit, and the Quality of RNA was checked by using Qubit Fluorometer 4 and Tapestatoin2200. QC passed RNA samples were considered for library preparation by using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina using manufacturer's protocols. The quality of the prepared library was determined by using Qubit 1X dsDNA Assay Kit and Agilent D1000 Screen Tape on TapeStation. Qualified libraries were considered further for sequencing using paired-end(PE) reads of 151bp length format on the Illumina Novaseq 6000 platform.