Bulk RNA-seq of trigeminal ganglia from C57BL/6 (wild-type) and Trpv1KO mice with uni- and bilateral dry eye (uni- and bilateral extraorbital lacrimal gland excision model) versus sham-operated controls
Bulk RNA-seq of trigeminal ganglia from C57BL/6 (wild-type) and Trpv1KO mice with uni- and bilateral dry eye (uni- and bilateral extraorbital lacrimal gland excision model) versus sham-operated controls
The trigeminal ganglia were dissected after euthanasia and cardiac perfusion with PBS to remove contaminating blood cells, collected in ice-cold TRI Reagent, and stored at -80°C until processing. both trigeminal ganglia from one mouse were homogenized in 1 ml of TRI Reagent, and then 0.2 ml of isopropanol was added. After centrifugation, RNA was purified from the aqueous phase using the Direct-zol RNA MiniPrep kit (Cat #R2052, Zymo Research, Irvine, CA, USA) following the manufacturer's instructions. The concentration and purity of RNA were assessed with a NanoDrop 1000 spectrophotometer (ThermoFisher  Scientific, Waltham,  MA,  USA). RNA-Seq was performed by NovoGene (Sacramento, CA, USA) using the Illumina NovaSeq platform to generate 150 bp paired-end reads.