Car2 (CRX1915838; CRA029050)
(1) Extract (ZymoBIOMICS DNA mini prep kit) and test quality of genomic DNA (2) Genomic DNA fragmentation(3) Repair the end of DNA fragments by adding base A at 3' end as viscous terminal(4) Add DNA adapter containing indexed sequence on both sides of the viscous terminal via base complementation(5) Collect target fragments in a certain range by Magnetic beads screening(6) Index the end of the target fragment with PCR amplification, and complete the construction and detection of the sequencing libraries(7) Bind the sequencing libraries to sequencing chips with Bridge PCR(8) Process sequencing in Illumina HiSeq/Nova 2x150bp submitted_instrument_model: Illumina HiSeq X Ten