GSM9792396: A549 cells, 8 Gy C-ions, chronic hypoxia, 4 h, replicate 4; Homo sapiens; RNA-Seq
RNA was harvested using RNeasy Mini kit (Qiagen). At least 3 µg total RNA per sample (4 biological repeats per condition, with RNA Integrity Numbers (RIN) above 9.0) were sent on dry ice to GENEWIZ (Leipzig, Germany) for mRNA sequencing in the same run after Poly(A) selection using the Illumina NovaSeq6000 platform (configuration: 2x150 bp, 350M read pairs) RNA libraries for RNA-seq were prepared by GENEWIZ.