GSM8580818: pNET, scRNA-seq; Homo sapiens; RNA-Seq
Fresh tumor tissues were mechanically chopped with scalpels on a plate and enzymatically digested with collagenase type IV (Servicebio) and DNAse I (Magen). Following digestion, the cell suspension was subjected to erythrocyte removal using the red blood cell lysis buffer (QuantoBio). Cell suspension was filtered through a 70 μm strainer (MACS), and dissociated cells were pelleted and re-suspended in phosphate buffer saline (PBS) with 0.04% BSA (bovine serum albumin). The Chromium Next GEM Single Cell 5' v2 Kit (10× Genomics, USA) and the Chromium Single Cell Controller Instrument were used to generate a 10× single-cell library according to the manufacturer's instructions.