GSM8863904: LN95-1; Homo sapiens; RNA-Seq
Total RNA was extracted from cells using the Purelink RNA mini kit (Invitrogen) following the manufacturer's standard protocol. Library Construction for DNBseq Sequencing: Total RNA was enriched for mRNA using Oligo(dT) selection, followed by fragmentation and reverse transcription into cDNA. The cDNA was then subjected to end repair, A-tailing, and adaptor ligation, followed by PCR amplification. Amplified DNA was denatured into single strands and circularized to generate the sequencing library. Finally, DNA nanoballs (DNBs) were synthesized and sequenced on the DNBseq platform.