GSM8970725: iHepatocyte from chr21-trisomic (T21) iPSC; Cell line 232_ihep_T21_1; Differentiation replicate 1; Homo sapiens; RNA-Seq
Total RNA was harvested using TRIzol reagent (Invitrogen) following the manufacturer protocol. RNA quality was assessed using an Agilent 2200 TapeStation and quantified by Qubit (Life Technologies). Poly-A(+) RNA enrichment, and strand-specific library preparation were carried out using a Tecan/Nugen Universal Plus mRNA Kit. Paired-end 150 bp sequencing was carried out on an Illumina NovaSeq 6000 instrument by the University of Colorado Cancer Center Genomics Shared Resource.