WGS of  Escherichia coli
The library preparation was performed using Nextera XT DNA Library Preparation Kit and Nextera XT Index Kit by Illumina according to the manufacturers instructions (Illumina, USA). In order to obtain the best results, a DNA input is recommended to be quantified and diluted to 1ng input (5 L of concentration 0.2 ng/L) in molecular-grade water. The DNA was tagmented using Nextera transposomes. Then it was amplified and tagged using the indices through a 12-cycle PCR. The samples were then normalized, pooled and subjected to 300-base paired-end read sequencing using an Illumina MiSeqDx system according to the manufacturers protocol.
SUB15154719
CRE080_S78