GSM8798259: CD8 T cells supplemented with 30 international units (IU) of IL-2 for 24 hours replicate 3; Mus musculus; RNA-Seq
Total RNA was extracted from cells using RNA isolation kit (Cat# 74104; QIAGEN). 2μg of total RNA was used for RNA sequencing. RNA samples were quantified using Qubit 2.0 Fluorometer (Life Technologies, 23 Carlsbad, CA, USA) and RNA integrity was checked using Agilent TapeStation 4200 (Agilent Technologies, Palo Alto, CA, USA). NEBNext Ultra II RNA Library Prep Kit (Illumina) was utilized for making RNA libraries and samples were sequenced by a 2x150bp Paired End (PE) configuration.
GEO
GSM8798259_r1