shotgun sequencing
The DNA extraction from sperm was performed using an E.Z.N.A. DNA/RNA Isolation Kit (Omega Bio-Tek). Quality control was performed using a NanoVue Plus Spectrophotometer (GE), a Qubit 4 Fluorometer (Invitrogen), and gel electrophoresis to verify suitable quantity, purity, and integrity of the DNA sample. Total genomic DNA was submitted to the University of Floridas Interdisciplinary Center for Biotechnology Research (UF ICBR) for library construction and sequencing. Quality control of the DNA sample was also evaluated by UF ICBR prior to library construction with a follow up quality and quantity assessment using a TapeStation System (Agilent).
SUB15088068
Illumina MiSeq