RNA-Seq of Xinhexiang1: seedling ,root, salt
Total RNA was extracted using CTAB and PBIOZOL. mRNA was enriched using Oligo(dT) magnetic beads. then first-strand cDNA was synthesized using random hexamer primers. Second-strand cDNA synthesis incorporated dUTP to achieve strand-specificity, followed by end repair and dA-tailing. Adaptors were ligated, and fragments of 250-350 bp were selected. The library was PCR amplified and purified. Sequencing was performed on an Illumina platform with paired-end reads.
SUB16108015
Salt_Root_1_1