GSM9032032: aPhp1_3; Porphyromonas gingivalis; RNA-Seq
Collected abscess material was briefly spun at  150 x g for 3 min at 4 degress Celsius to pellet any mouse cells present. The supernatant was transferred to a new tube and spun at 5000 x g for 5 min at 4 degrees Celsius to pellet bacteria cells. The supernatant was removed and cell pellet resuspened into TRIzol™ Reagent (Invitrogen cat #15596026) and total RNA extraction was caried out using the Qiagen RNeasy Plus Mini Kit (Qiagen cat #74136) following manufacturer protocol including an optional DNAse treatment step and eluting into nuclease-free water. Each sample of total RNA suspended in nuclease-free water was then shipped on dry ice to a commercial vendor for RNA sequencing. Library construction was performed by Novogene. In brief, a strand-specific library was constructed with rRNA removal for 150-bp paired-end sequencing on an Illumina NovaSeq 6000.
GEO
GSM9032032_r1