M-RS5-3
Following successful sample DNA testing, a proportionate amount of lambda DNA was added as a quality control for bisulfite conversion efficiency. Genomic DNA was randomly sheared into 200-400bp fragments using the Covaris M220 system. The sheared DNA fragments underwent end-repair, A-tailing, and ligation with methylation-modified sequencing adapters. Subsequently, bisulphite conversion was performed (using the EZ DNA Methylation Gold Kit, Zymo Research). Following this process, unmethylated cytosines (C) converted to uracil (U) (which became thymine (T) after PCR amplification), while methylated cytosines (C) remained unchanged. Finally, PCR amplification yielded the final WGBS library.
SUB15104252
M-RS5_3.1