Base editing screen of Msh2
Base editing mutagenesis screen was performed on Msh2 in haploid mouse embryonic cells. Loss-of-function mutations were selected with 6-Thioguanine. RNA was extracted using Rneasy (QIAGEN), cDNA was synthesized using PrimeScript RT Kit (Takara), the coding region of Msh2 was amplified using Q5 polymerase (NEB). Libraries were prepped from PCR amplicons using Nextera XT Kit (Illumina) and sequenced on MiSeq system (Illumina).
SUB15341166
Msh2_mut_unselected