Synergistic co-digestion of sugarcane bagasse and swine manure for enhanced CH4 production with insights into microbial dynamic
Samples of 2.0 mL, collected at the end of the operational period of assays R2 and R4, were stored at -20 C until genomic DNA extraction, which was performed using the FastDNA SPIN Kit for Soil (MP Biomedicals), following the manufacturers instructions. Oligonucleotide sequencing and basic bioinformatic analyses for taxonomic identification were carried out by NGS Solues Genmicas (Piracicaba, SP, Brazil). Microbial characterization was obtained through amplification of the 16S rRNA gene using primer pairs 341F785R for the Bacteria domain and Ar915aFAr1386R for the Archaea domain. Sequencing data, obtained using the Illumina platform, were processed in the R environment with specific packages for phylogenomic analyses. Taxonomic classification was performed based on the SILVA database.
SUB15794936
R2_ARC