GSM9217640: RAW 264.7 cells, 4 hr TBHQ treatment, 12 hr LPS stimulation, rep 3; Mus musculus; RNA-Seq
RNA was isolated using the Direct-zol RNA Miniprep Kit (R2051A, Zymo Research, Irvine, CA, USA) according to the manufacturer's protocol. Initial quality control, read alignment to Mus musculus reference genome (GRCm38/mm39), transcript assembly, and normalization using fragments per kilobase of transcript per million mapped reads (FPKM) were performed by Novogene Corporation, Inc. (Sacramento, CA, USA). Briefly, directional and non-directional libraries were prepared from mRNA purified from total RNA using poly-T oligo-attached magnetic beads. cDNA was synthesized after fragmentation using random heamer primers and dUTP/dTTP for second strand cDNA synthesis. End repair, A-tailing, adapter ligation, size selection USER enzyme digestion (directional library), amplification, and purification were performed thereafter.
GEO
GSM9217640_r1