MinION sequencing
Amplicons were cleaned-up with 1.8X volume of Agencourt AMPure beads (Beckman Coulter)  and amplicon concentrations were quantified with the Qubit 1X dsDNA HS Assay Kit (Invitrogen). Library preparation was performed using the Oxford Nanopore Technologies (ONT) Ligation Sequencing Kit V14 (SQK-LSK114).
Ligation Sequencing Kit V14 (SQK-LSK114)
The Biotechnology Centre, University of Yaounde 1
ena-EXPERIMENT-TAB-23-01-2026-11:47:40:537-506251