Potter Cove sediment slurry SIP incubation - 12C Acetate.DIC.molybdate - RNA midpoint fraction - Bacteria 16Sr RNA
RNA was extracted from slurry with a phenol-chloroform protocol. RNA was density separated into 14 fraction by ultra-centrifugation. cDNA synthesis was done on selected fractions. PCRs were performed using primers with unique 8 bp barcodes at forward and reverse primers. Multiple samples were pooled into one library for sequencing. During demultiplexing reads were sorted by read direction forward-reverse or reverse-forward using the primer sequence, resulting in multiple paired fastq files (=runs) for the same ENA experiment
University of Bremen
12ADM.8_Bacteria_16SrRNA_Lib82