PacBio HIFI of Vanilla planifolia : axillary buds
Library preparation and sequencing were performed according to the manufacturers instructions Procedure & Checklist  Preparing whole genome and metagenome libraries using SMRTbell prep kit 3.0. Purification steps were performed using AMPure PB beads (PacBio) and SMRTbell cleanup beads (PacBio). A repair stage was carried out on 10g DNA using SMRTbell Damage Repair Kit SPV3 (PacBio) then sheared at 15 kb (speed V33) using the Megaruptor3 system (Diagenode). After a nuclease step, the library was size-selected using a 6 kb cutoff on the Pippin HT Size Selection system (Sage Science) with 0.75% Agarose, 6-10 kb High Pass, 75E" protocol. To obtain sufficient material, this library preparation was again carried out on 10 g. The only difference is that the fragmentation was performed this time on the Megaruptor1 system at 20 kb.
SUB15304954
HIFI_CCS_5