GSM8899164: αLPs from bone marrow of WT mice; Mus musculus; RNA-Seq
To obtain Lin−CD45+CD127+Flt3−α4β7+CD25− αLPs subsets from fetal liver and bone marrow of WT B6  mice, the  mononuclear cells (MNCs) were incubated with anti-CD45 mAbs for 30 min at 4°C. Subsequently, MNCs were incubated with anti-PE beads for 60 min at 4°C. Nextly, MNCs were incubated with the indicated antibodies for 30 min at 4°C. 7AAD was added to the MNCs suspension, which was subsequently sorted using a FACSAria Fusion (BD Biosciences, San Diego, USA). The purity of the sorted cell populations was >  90%. Purified live cells were labelled with 0.4% Trypan blue to determine their viability (> 90% before processing for scRNA-seq). Single-cell barcode scRNA-seq libraries were generated using the Chromium Single Cell 3' Library (v2 chemistry; Nugen) according to the manufacturer's standard protocol. The libraries were sequenced on a NovaSeq 6000 (Illumina). Gene-barcode matrices were generated by counting UMIs and filtering non-cell-associated barcodes.
GEO
GSM8899164_r1