A-2 (CRX1871618; CRA028244)
Using RNA as template, first-strand cDNA was synthesized with 6-base random primers and reverse transcriptase, followed by second-strand cDNA synthesis using the first strand as template. After library construction, library fragments were enriched by PCR amplification and selected by fragment size, with the library size being 450 bp.
Gansu Agricultural University
DRX799060
NGDC
CRX1871618