metagenomics of ocean water column from the Gulf of Mexico
DNA was extracted from the disc filters using an enzymatic lysis and phenol protocol as previously described (Tsementzi et al., 2014). Briefly cells were lysed with the addition of lysozyme (1mg/l final concentration in 5ml of lysis buffer per filter) and incubation for 30 min at 37oC. Addition of Proteinase K (1mg/100l lysis buffer with 100l 20% SDS) was followed by a 2h incubation at 55oC. DNA was extracted by phenol:chloroform extraction, followed by ethnol precipitation and wash. DNA was purified using the Ampure XP-Beads
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