GSM9217198: –2 timepoint of the second day of the transition from diurnal LL to HL, replicate 2; Chlamydomonas reinhardtii; RNA-Seq
Roughly 17 mL of culture was collected by centrifugation at 2500 × g for 2 min at 4°C. Cell pellets were resuspended in 750 µL Zymo DNA/RNA Shield and transferred to ZR BashingBead Lysis Tubes containing 0.1 mm and 0.5 mm beads. Samples were subjected to bead beating for 2 min in a Mini-BeadBeater 16 Cell Disrupter in a 4°C room, and then were processed with the ZymoBIOMICS RNA Mini Kit according to the manufacturer's instructions. Standard Illumina RNA-Seq kits.
GEO
GSM9217198_r1