GSM9835699: Cortical NPCs, WT, Rabbit IgG control CUT&RUN, Replicate 2; Mus musculus; OTHER
CUT&RUN was performed according to Epicypher CUTANA protocol (v.1.7) on 300.000 cells per sample. We permeabilized the cells with 0.01% digitonin (Sigma, D141). For normalization we spiked in E. Coli DNA (Epicypher, 23618-1401) at the final concentration of 0.2 ng per sample. CUT&RUN: The library preparation was performed with the TrueSeq-Chip Sample preparation Kit (Illumina, IP-202-9001). Libraries were sequenced using on NovaSeq 6000 S4 with a minimum depth of 12 million paired end reads per sample.
GEO
GSM9835699_r1