RNA seq of Homo sapiens: primary human embryonal kidney treated with amiRNA
Total RNA was isolated from HEK293T cells treated with amiR136-13A. Small RNA libraries were prepared from total RNA using the TruSeq Small RNA Library Prep Kit (Illumina) following the manufacturers instructions, starting from 1 g of RNA and performing 11 PCR enrichment cycles.Library size was assessed with an Agilent TapeStation 2200 using the High Sensitivity D1000 reagents, and concentrations were determined by qPCR with the KAPA Library Quantification Kit.Sequencing was performed on an Illumina NovaSeq 6000 platform.
SUB15722306
HEK293T_amiR136-13A