ITS barcoding
Genomic DNA from chocho samples was analyzed using metagenomic sequencing to profile both bacterial and fungal communities. The fungal ITS2 region was amplified using primers ITS86F (5-GTGAATCATCGAATCTTTGAA-3) and ITS4 (5-TCCTCCGCTTATTGATATGC-3) (Toju et al., 2012). PCR reactions were performed using KAPA HiFi HotStart ReadyMix (Sigma Aldrich, USA). Illumina sequencing adapters and dual index barcodes were added to the target amplicons, followed by library purification with magnetic beads. Sequencing was conducted on Illumina Novaseq.
SUB15722494
KR2