sequencing of bovine viral diarrhea virus from cattle
Viral RNA was extracted from purified viruses using TRIzolTM LS Reagent. The double-stranded complementary DNA was synthesised with modifications and sequenced using both Illumina and Oxford Nanopore Technologies. The Illumina library was prepared using the Illumina Nextera XT library preparation. The two samples were pooled at equal molarity and used in a single Illumina MiSeq Nano 2X 150 base paired-end run using version 2 chemistry.
SUB15517591
Ill_Bovax