GSM8898890: db/db_scATAC-seq_replicate 1; Mus musculus; RNA-Seq
We pooled aortas from 8 mice into two replicates (4 mice per replicate) for each of the three groups. Aortas (4/group) were cut into ~1 mm pieces and placed into enzyme mixture containing Liberase DH (1Unit/aorta, 5401054001, Roche), Elastase (0.125 mg/aorta, E0127, Sigma) and DNase I (Kunitz unit/aorta, 79254, Qiagen) in M199 medium (10060CV, Corning). After incubating at 37°C for 45 minutes, cell suspensions were filtered through a 70-µm strainer and centrifuged at 500g for 5 min. The cell pellets were washed with Hank's balanced salt solution (HBSS) and FACS buffer (HBSS containing 2% FBS) and resuspended in 500 µL of FACS buffer and passed through 35-µm strainer. Cell viability was measured using the Acridine Orange/Propidium Iodide method (Cellometer, Nexcelom Biosciences). Ten thousand nuclei were processed according to 10X Genomics' standard protocol using Chromium Next GEM single cell ATAC reagent kits v1.1, Version CG000209 Rev F kit according to the manufacturer's protocol.
GEO
GSM8898890_r1