16S-Seq of mouse cecal: HFD2
DNA extraction, frozen cecal samples were thawed at room temperature, and 250 mg aliquots of cecal material were lysed and homogenized in a bead-beating tube containing 0.1 mm and 0.5 mm beads and ZymoBiomics lysis buffer using Omnis Bead Ruptor 96 (Perkin Elmer, USA) following manufacturers instructions. Total DNA was then isolated using the ZymoBiomics DNA extraction mini kit (Zymo Research, USA) with a final elution volume of 50 l. The concentration of genomic dsDNA was measured for each sample using Qubit dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA) on an Invitrogen Qubit 4.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA).
SUB15305063
EMC3155