GSM9690995: 51069 WT Europeans; Homo sapiens; RNA-Seq
Total RNA was extracted from brain tissue and cultured cells using the Qiagen RNeasy Mini Kit according to the manufacturer's instructions. RNA quantity was measured by Qubit RNA HS Assay and quality assessed using an Agilent Bioanalyzer. Targeted full-length MAPT cDNA libraries were generated from 100 ng total RNA. First-strand cDNA synthesis was performed using oligo(dT)-primed reverse transcription. Gene-specific PCR amplification of MAPT transcripts was performed, followed by AMPure PB bead purification. Barcoded SMRTbell libraries were prepared using the PacBio SMRTbell Express Template Prep Kit and sequenced on the PacBio Sequel II platform in CCS mode to generate HiFi reads.
GEO
GSM9690995_r1