GSM9386581: Capan-2 Day7 replicate 1; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNeasy Mini Kit 21 days post-induction. Samples with an A260/A280 ratio between 1.8-2.0 as determined by NanoDrop were selected for subsequent experiments. cDNA was synthesized using the High-Capacity cDNA Reverse Transcription Kit. mRNA was enriched using oligo(dT) magnetic beads followed by fragmentation. First- and second-strand cDNA synthesis was performed, and sequencing libraries were constructed using the Illumina TruSeq mRNA Library Preparation Kit following the manufacturer's protocol. Libraries were subjected to PCR amplification and size selection prior to sequencing.
GEO
GSM9386581_r1