RNA-Seq of RadishBrassica root
Total RNA was extracted from RaphanusBrassica roots using TRIzol, followed by DNase I treatment and polyA selection with Oligo-dT beads. Strand-specific RNA-Seq libraries were prepared via dUTP-based second-strand marking, fragmented cDNA adapter ligation (Illumina TruSeq), and 15-cycle PCR amplification. Libraries were quantified (Qubit 4.0) and size-verified (Agilent Bioanalyzer), then sequenced as 150-bp paired-end reads on the Illumina NovaSeq X Plus platform, with three biological replicates per experimental group.
SUB15149287
RRC5-1