GSM8695222: E14.5_MGE_YAP_TAZ_CONTROL_3; Mus musculus; RNA-Seq
MGEs were obtained by first extracting the embryonic brain and detaching the telencephalon, followed by removing the dorsal telencephalon and finally dissecting the MGE from the rest of the subpallium in cold PBS. Both MGEs from the same embryo were dissociated using either Stem-Pro Accutase (Thermo Scientific A1110501) or a papain dissociation kit (Worthington LK003150) for less than 5 min. Cells were filtered through Flowmi cell strainers (SP Bel-Art #13680040). Cell viability was analyzed with a Countess cell counter and only samples with a viability of >90% were processed for scRNAseq. A 3' scRNAseq kit from 10x Genomics was used according to kit instructions. Up to 10K cells were loaded per each bead mix.
GEO
GSM8695222_r1