Shotgun metagenomic sequencing of Homo sapiens: newborn blood
Genomic DNA was extracted from patient's blood by using Quick-DNA Magbead Plus DNA Kit (Zymo Research, USA). DNA samples analyzed for concentration and purity using the Qubit Fluorometer 4.0 and Implen NanoQuant Spectrophotometer; processed using the Illumina DNA Prep kit (Illumina, CA, USA) according to manufacturers instruction. DNA libraries were analyzed for concentration and average library size using the Qubit Fluorometer 4.0 and Lab Chip GX Touch Nucleic Acid Analyzer, respectively. Samples were sequenced on Illumina NovaSeq 6000 system (Illumina, CA, USA) system. Libraries were normalized to 1.0 nM and pooled before loading into the NovaSeq 6000 sequencing platform. The paired-end reads were demultiplexed using the Illumina DRAGEN v4.3 (Dynamic Read Analysis for GENomics) Bio-IT Platform.
SUB16245921
P. urinalis BCR1