RNA-seq of keratinocytes with siRNA against control.
QuantSeq mRNA-seq V2 (LEXOGEN SKU: 191.96) library prep, using the UMI (Lexogen SKU: 081.96), of primary kerationocytes. 2.5µL of 100µM each siRNA was transfected to 10cm plates containing 1.3M resuspended human primary keratinocytes, using Lipofectamine RNAiMax (Invitrogen, 13778150). After 24 hours, new 50:50 media was added. Cells were seeded for differentiation or further progenitor growth after 48 h transfection. The negative control was the ON-TARGET Smart pool negative control (Horizon, D-001810-10-20). Total RNA was extracted using the RNeasy Plus kit (QIAGEN, 74136).
NHGRI-phs003977
new_control_Prog_siRNA_30