GSM8834340: HEK293T, shZNF384,rep2; Homo sapiens; RNA-Seq
Nuclear RNA were isolated according to the manufacturer's instructions (Vazyme, E101).Total RNA was extracted using the Ultrapure RNA Kit(CWbio, CW0581S). according to the manufacturer's protocol. RNA quality was assessed on an Agilent 2100 Bioanalyzer (Agilent Technologies) and checked using RNase free agarose gel electrophoresis. mRNA was enriched by Oligo(dT) beads. Then, the enriched mRNA was fragmented using a fragmentation buffer and reversely transcribed into cDNA by NEBNext Ultra RNA Library Prep Kit for Illumina.
GEO
GSM8834340_r1