GSM9324999: U2OS cells ZNF354A siRNA_2; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNeasy kit (Qiagen) according to the manufacturer's instructions. RNA integrity was confirmed with a TapeStation 4200 (Agilent). Poly(A)+ mRNA libraries were prepared from 800 ng total RNA using the Illumina Stranded mRNA Ligation kit. Libraries were indexed and sequenced on an Illumina NovaSeq 6000 platform to generate 2 × 60 bp paired-end reads (~70 million read pairs per sample).
GEO
GSM9324999_r1