RNA seq of Sus scrofa: muscle sample
Total RNA was extracted from muscle samples, and mRNA was purified with poly-T magnetic beads. The mRNA was fragmented and converted into cDNA using random hexamer primers and reverse transcriptase. The second strand was synthesized, blunt ends created, and adaptors ligated. cDNA fragments of 370-420 bp were purified and amplified using PCR.
SUB15092442
NJ3_RNAseq