GSM9439317: Patient BM050038: Proteomic and transcriptomic profiling identifies TIPRL as a potential target in MM with 1q gain; Homo sapiens; RNA-Seq
Bone marrow samples were collected after approval from the Institutional Review Board at Mayo Clinic, Rochester. CD138+ plasma cells were enriched from bone marrow aspirates and processed using AllPrep DNA/RNA/Protein extraction kit (Qiagen) using manufacturer's protocol for DNA and RNA extraction. The flow-through from the RNA extraction step was precipitated with advanced protein precipitation buffer (Qiagen) to obtain the protein fraction and the protein pellets were stored at -80˚C until further processing. RNA libraries were prepared using the TruSeq RNA sample prep kit v2 (Illumina, San Diego, CA) according to the manufacturer's instructions.
GEO
GSM9439317_r1