RNA Pol II (total) CUT and RUN on RG purified from GW17/20 cerebral cortex
Radial glia (RG) were purified from human cortical brain tissue at gestational weeks (GW) 17/20 using flourescence activated nuclei sorting (FANS).  Sorted nuclei were incubated with Fab fragments to block antibodies used in FANS and ~50,000 nuclei were  subjected to CUT&RUN using  antibodies to Pol II CTD (total) . Sequencing libraries were constructed using KAPA Hyperprep kit following manufacturer's instructions.  Libraries were sequenced using 150-bp paired-end sequencing on Illumina NovaSeq X Plus
NIMH-phs000989
GW1720_RG_Pol II CTD