GSM9653650: R_rep2; Homo sapiens; RNA-Seq
Total RNA was extracted from intestinal epithelial cells following 110 minutes of infection with Vibrio vulnificus strains or PBS control. Cells were lysed and RNA was isolated using a standard column-based RNA purification method according to the manufacturer's instructions. RNA-seq libraries were prepared from total RNA using a poly(A) selection protocol to enrich for mRNA, followed by fragmentation, cDNA synthesis, adapter ligation, and PCR amplification according to the manufacturer's instructions.
GEO
GSM9653650_r1