GSM8899891: Internal sample 1; Homo sapiens; RNA-Seq
For tumor dissociation, gentle MACS Human Tumor Dissociation Kit (Miltenyi Biotec) was used, and single-cell library preparation was conducted according to the manufacturer's guidelines (10X Genomics). Gene expression libraries were generated using the Chromium Single Cell 5′ Reagent Kits (10X Genomics), targeting a capture of 8000 to 10,000 cells. The TruSeq Stranded Total RNA Library Prep Globin Kit (Illumina) was used for RNA sequencing libraries construction and sequenced on the NovaSeq X platform (Illumina). Sequencing reads were performed for quality assessment using FastQC v0.11.7, and adaptor trimming and low-quality read removal were performed using Trimmomatic v0.38. Filtered reads were aligned to the GRCh38 (human) and mm10 (mouse) reference genomes using HISAT2. BAMCMP was utilized to separate human and murine reads, retaining only human-derived reads (GRCh38) for downstream analysis.
GEO
GSM8899891_r1