GSM9769598: Case1_BLBC; Homo sapiens; RNA-Seq
scRNA-seq was performed on the BM mononucleated cells (BMMCs) from the chronic phase and B-lymphoblastic crisis samples collected from three patients using the chromium system (10X Genomics). Single-cell gene expression libraries were prepared according to the Chromium NextGEM Single Cell Gene Expression 3v3.1 kit (10x Genomics). Briefly, single cells, reverse transcription reagents, gel beads containing barcoded oligonucleotides, and oil were loaded on a Chromium controller (10x Genomics) to generate single-cell GEMs (Gel Bead-In-Emulsion). Full-length cDNA was synthesized and barcoded within each GEM. Subsequently, GEMs were broken, and cDNA was pooled. Following cleanup using Dynabeads MyOne Silane Beads, cDNA was amplified by PCR. The amplified product was fragmented prior to end-repair, A-tailing, and adaptor ligation.
GEO
GSM9769598_r1