GSM9323921: Splenic CD8+ T cells, rested, 24-hours, Dusp2-floxed control, mouse 1; Mus musculus; RNA-Seq
Total RNA was isolated with TRIzol Reagent (Invitrogen), prior to inclusion in spin-column based isolation (Zymo Research). 100 ng of total RNA was used for construction of cDNA libraies. Libraries were prepared with Illumina Stranded mRNA Prep kit following manufacturer's instructions. The concentration and size distribution of the completed libraries were determined with the Agilent TapeStation's D1000 ScreenTape and Qubit fluorometry.
GEO
GSM9323921_r1