GSM9085118: Kras_Q61R_mouse_3_tumor_1; Mus musculus; OTHER
Genomic DNA was isolated using the QIAmp DNA FFPE Tissue Kit (Qiagen 56404). Exome capture was performed using 0.5ug of genomic DNA. Exome capture was performed using 0.5mg of genomic DNA with the SureSelect XT mouse all exon kit according to manufacturer's protocol (Agilent Technologies). The fragment size distribution of post-capture amplified libraries was determined with 2200 TapeStation using high sensitivity D1000 screen tape (Agilent Technologies). Concentration of the libraries was measured by a Qubit fluorometer (Thermo Fisher Scientific). Exome capture libraries were sequenced on HiSeq 4000 system (Illumina) to generate 50 million paired-end 75-base-pair reads.
GEO
GSM9085118_r1