GSM9501433: Visium of tumor and adjcent liver tissues Chow anti-PD-1 A1; Mus musculus; OTHER
The isolated RNA was quality-checked using Agilent RNA 6000 Pico kit (#5067-1513) by Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, USA). DV200 of the RNA samples need more than 30% to qualify for the assay. The qualified samples after H&E staining and image were decrosslinked based the protocol (CG000409) and the library preparation followed the protocol (CG000407). The quality of the final libraries was examined using Agilent High Sensitive DNA Kit (#5067-4626) by Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, USA), and the library concentrations were determined by qPCR using Collibri Library Quantification kit (#A38524500, Thermo Fisher Scientific). The libraries were pooled evenly and went for the paired-end 75-cycle sequencing on an Illumina NextSeq 550 System (Illumina, Inc., USA) using High Output Kit v2.5 (#20024907, Illumina, Inc., USA).
GEO
GSM9501433_r1