Amplicon sequencing of metagenome: shiitake mushroom residue compost
Total DNA was extracted from shiitake mushroom residue compost samples. Primers 338F_806R and ITS1F_ITS2R were used for PCR amplification of 16S rRNA gene and ITS region (multi-primer for microbial diversity). Purified, quantified PCR products were used for library construction following standard Illumina protocol with paired-end layout before sequencing.
SUB16026302
A4_1