GSM9085340: dHyperLbCas12a-4xSID, Day 14, Rep 2; synthetic construct; OTHER
Plasmid libraries were purified using the Qiagen midi plus kit. Genomic DNA was extracted from cell pellets using a Puregene kit (Qiagen) crRNA arrays were amplified from genomic or plasmid DNA using KAPA HiFi 2x master mix (Roche). Primers contained Illumina adapter sequences and indexes. Standard Illumina primers were used for sequencing
GEO
GSM9085340_r1