GSM8659449: Fixed scRNAseq,multiplexed pool 2; Mus musculus; RNA-Seq
ChATflox and ChATflox;Mb1-Cre mouse livers were collected at 2, 4, and 24 hours after PHX or Sham surgery, and 25mg of tissue from the same lobe across experimental samples were immediately snap-frozen. Thawing and dissociation of cell samples were performed according to the Tissue Fixation & Dissociation for Chromium Fixed RNA Profiling protocols provided by 10X Genomics. Sample probe hybridization, washing, pooling, and capture of approximately10,000 cells per sample, 4 samples per library (2 libraries), and followed by library construction, were performed according to 10X Genomics protocols for the 4-plex kit.
GEO
GSM8659449_r1