nirK1aCuF_nirKR3CuR Groups C Replication 1
Total soil DNA was extracted using the FastDNA Spin Kit for Soil (MP Biomedicals, Solon, OH, USA). DNA concentration and purity were assessed using a NanoDrop 2000 spectrophotometer, and DNA quality was checked by 1% agarose gel electrophoresis. Two functional-gene amplicon datasets were generated from the same 12 soil samples. The nifH gene was amplified using the nifHF/nifHR primer pair, and the nirK gene was amplified using the nirK1aCuF/nirKR3CuR primer pair. PCR amplification was performed using TransGen AP221-02 TransStart FastPfu DNA Polymerase. PCR products were checked by 1% agarose gel electrophoresis and purified using Agencourt AMPure XP. Purified amplicons were subjected to paired-end high-throughput sequencing on an Illumina amplicon sequencing platform.
SUB16216950
A25284841d