GSM9114546: BMDM, W3; Mus musculus; RNA-Seq
Total RNA was extracted using TRIzol and the quality and quantity of total RNA were evaluated using an Agilent 2100 Bioanalyzer. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection.
GEO
GSM9114546_r1