Fecal sample shut gun sequencing of Pre-Metformin+Liraglutide subject 4
The extracted and quantified DNA (150 ng) was used for library preparation using Illumina DNA Prep, (M) Tagmentation kit (Illumina, Inc, 5200 Illumina Way, San Diego CA, USA) by following the manufacturers instructions. Additionally, sample specific unique IDT for IlluminaNextera DNA UD Indexes were used. The sequencing was done on Illumina NextSeq1000 machine using an Illumina NextSeq 1000/2000 P2 Reagents (300 Cycles) v3 reagent cartridge (Illumina, Inc, 5200 Illumina Way, San Diego CA, USA).
SUB15407810
R24_S31