ITS of LEO soil fungal community: S2CM_ITS
DNA was extracted from approximately 250 mg of homogenized soil using the FastDNA Spin Kit for Soil (MP Biomedicals, USA) following the protocol. DNA quantity and quality were assessed using a Qubit dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific, USA). The fungal internal transcribed spacer region (ITS1) was amplified using the primer pair ITS1F (5'-CTTGGTCATTTAGAGGAAGTAA-3') and ITS2 (5'-GCTGCGTTCTTCATCGATGC-3'). Amplicon libraries were prepared following the same PCR conditions as the 16S libraries and sequenced on an Illumina MiSeq platform using paired-end 2  150 bp chemistry.
SUB16050555
S2CM_ITS_paired_illumina