Metagenomic shotgun microbiome sequencing of human stool
DNA from fecal samples were isolated using the QIAGEN DNeasy PowerSoil Pro Kit. DNA libraries were prepared using the Illumina Nextera XT library preparation kit and the library quantity assessed with Qubit (ThermoFisher). Libraries were sequenced on an Illumina HiSeq platform 2x150bp. The average sequencing depth was 4,948,936 reads per sample (range: 1,240,758-14,788,148). Taxonomic assignment and functional analysis were performed using the CosmosID bioinformatics platform. Taxonomy was assigned using the kmer-based algorithms and the GenBook database, which comprises >150,000 bacteria, viruses, fungi and protists genomes.
SUB14888249
JNEU-0023_CP05336