Shot-gun metagenome of Halisarca dujardinii sponge, 2020 (ONT)
Genomic high-molecular-weight (HMW) DNA was extracted from 1 g of H. dujardinii tissue using the phenol-chloroform method following standard protocols, with additional purification performed using the CTAB method to ensure high-quality DNA. The DNA was further size-selected using the Circulomics Short Read Eliminator (SRE) Kit, following the manufacturer's protocol, to enrich for long DNA fragments. Sequence libraries were prepared using the Ligation Sequencing Kit SQK-LSK109, following the manufacturer's protocol. Sequencing was conducted for 48 hours using a MinION Flow Cell (FLO-MIN106D, R9.4.1 version). The MinKNOW software package (version 19.12.5) was used for basecalling and demultiplexing the reads. Sequencing was performed three times, and the resulting data were combined for analysis.
SUB15321912
Hal_nanopore