GSM9083948: BMSC CKD 4; Rattus; RNA-Seq
Total RNA was extracted from exosome samples using TRIzol Reagent (Invitrogen) according to the manufacturer's protocol. RNA quality and concentration were assessed using a Qubit®2.0 Fluorometer and Agilent 2200 TapeStation RNA integrity was verified, and samples with OD260/OD280 ratios between 1.8–2.0 were used for sequencing. RNA libraries were constructed using the Illumina TruSeq Stranded mRNA Library Prep Kit. Poly-A mRNA was purified from total RNA using oligo(dT) magnetic beads and fragmented. First and second strand cDNA synthesis was performed, followed by end repair, A-tailing, adapter ligation, and PCR amplification. The resulting libraries were quality-checked using the Agilent 2100 Bioanalyzer and quantified before sequencing on an Illumina platform.
GEO
GSM9083948_r1