WGS of Mus musculus: tail or ear punch tissue sample
DNA was extracted from ear punch, tail cip or muscule using Gentra Puregene Tissue Kits. NanoDrop Spectrophotometer was used to measure the concentration of the extracted DNA and was also used to assess the DNA quality by examining 260/280 and 260/230 absorbance ratios. Prior to library preparation, the presence of high-molecular weight DNA was confirmed using agarose gel electrophoresis. Library preparation and sequencing were conducted by the University of Wisconsin-Madison Biotechnology Center. Sequencing libraries were constructed with the Illumina DNA Library Prep using 100-500 ng of input DNA per sample. Purified and pooled libraries were sequenced on the Illumina NovaSeq6000 platform to generate paired-end 150 bp reads. Each mouse was sequenced across multiple lanes, ranging from 2-7 lanes per individual.
SUB15113845
FSa10