Genome sequencing of Hong Kong SAR ASFV isolates, 2023-2024
Virus DNA was extracted from concentrated DNAase treated virus obtained from infected bone marrow macrophage cultures exhibiting >90% cytopathic effect. The resulting DNA was extracted using the MagAttract high molecular weight DNA kit (Qiagen) and then amplified using REPLI-g (Qiagen). The presence of ASFV DNA was confirmed by qPCR (King et al., 2003) and only DNA with > 10E6 genome copies were used to generate libraries with approximately 2 ug DNA.
SUB15589051
HK3_nano