GSM9492940: Hashed, LARRY-barcoded AML cells in vivo, treated mice, day 40, 3' mRNA library; Mus musculus; OTHER
Samples were incubated with TotalSeq-B cell hashing antibodies (2 ul per 1M cells) for 30 minutes on ice, washed, and then sorted using a Beckman Coulter CytoFlex SRT sorter. Live GFP+ cells were sorted, centrifuged 800g for 10 minutes at 4ºC, and then resuspended in PBS-0.01%BSA buffer, counted for viability and concentration, and then single-cell processed using Chromium X (3' Gene Expression + Feature Barcoding kits). 10x 3' Gene Expression with Feature Barcoding v3.1 following manufacturer instructions. LARRY and CROPseq barcoding libraries were prepared as previously published (Datlinger et al. Nat Methods 2017, doi: 10.1038/nmeth.4177, and Singh et al. Cell Stem Cell 2025, doi: 10.1016/j.stem.2025.01.012)
GEO
GSM9492940_r1