A9-11-2
Simplified Library Preparation and Sequencing Protocol  DNA Extraction: Total metagenomic DNA was extracted from fecal samples using a commercial kit. Quality and concentration were checked.  Full-Length 16S rRNA Gene Amplification: The target region was amplified from the DNA using universal bacterial primers (e.g., 27F/1494R) in a high-fidelity PCR. The amplicons were purified.  PacBio SMRTbell Library Construction: Purified PCR products were repaired and ligated to hairpin adapters to form SMRTbell libraries, following the manufacturer's protocol. The final library was size-selected and quantified.  Sequencing: The library was sequenced on a PacBio Sequel II/IIe or Revio system using Circular Consensus Sequencing mode to generate high-accuracy reads.  This is a concise version for quick reference. If you need a more detailed version including specific kit names, instrument parameters, or QC thresholds, I can provide that as well.
SUB15855093
A9-11-2