GSM9440881: mRNA_TC2_WT_9h; Caenorhabditis elegans; RNA-Seq
At the time of sample collection, animals were washed off the plates with M9 buffer, washed twice with M9 buffer, resuspended in TRI Reagent (MRC, TR-118) and snap-frozen in liquid nitrogen. Prior to RNA extraction, animals were lysed by five cycles of thawing (42°C) and snap-freezing in liquid nitrogen. RNA was extracted using Direct-zol RNA Microprep kit (Zymo Research; R2062; DNase treatment included) according to the manufacturer's protocol. RNA was eluted in nuclease-free water (Thermo Fisher Scientific, Ambion, AM9937). TruSeq Illumina mRNA-seq
GEO
GSM9440881_r1