GSM9769650: LUAD stage III tumor sample T2B; Homo sapiens; RNA-Seq
Tissues were minced into small pieces and enzymatically dissociated using a mixture of collagenase and DNase I. The resulting cell suspension was filtered through a 40 μm strainer to remove debris and aggregates. Red blood cells were lysed using ACK lysis buffer. Viable single cells were collected and resuspended in PBS with 0.04% BSA. Single-cell RNA-seq libraries were constructed using the Chromium Next GEM Single Cell 3' Kit (v3.1, 10x Genomics) following the manufacturer's protocol. Briefly, single-cell suspensions were loaded onto a Chromium controller to generate single-cell Gel Beads-in-Emulsion (GEMs). After reverse transcription, cDNA was amplified and indexed libraries were prepared.
GEO
GSM9769650_r1