S_pombe_medium_centromere_RNA-seq_rep2
Cells grown to OD600 ~0.5 in fresh YEL broth prior to extraction. RNA extracted with hot phenol + chloroform/isoamyl alcohol extraction method from Bähler and Wise, 2017. RNA cleanup was performed with the RNA Clean & Concentrator kit (Zymo Research). Ribosomal RNAs were depleted with FastSelect Yeast reagent (Qiagen) and RNA libraries were prepared with RNA-Seq FastSelect Depletion (stranded) (Watchmaker). 75bp paired-end sequencing was performed on libraries via the AVITI sequencer (Element Biosciences).
SUB16020557
library8