18S rRNA marker gene survey of montaine fishes-Sculpin-intestine
To generate taxonomic and proportional abundance profiles for each sample, the V9 region of the 18S rRNA gene was amplified by PCR using the earth microbiome project primers 1391F (5- GTACACACCGCCCGTC-3)(Stoeck et al, 2010) and EukBrR (5- TGATCCTTCTGCAGGTTCACCTAC-3) )(Stoeck et al, 2010). Sequencing was performed on the Illumina MiSeq using a V2 2x250bp cycle standard flow cell
SUB14999982
Sc9Oct620221Int