GSM8607954: hMSCs in less dynamic hydrogel, H3K18la, ChIP; Homo sapiens; ChIP-Seq
Chromatin Immunoprecipitation Protocol 1. Add final concentration of 1% Formaldehyde solution for Cross-Linking of DNA and Protein. Then stoped the cross-linking reaction by adding final concentration of 125mM glycine. 2. Extract chromatin from cross-linked cells by adding Lysis Buffer. 3. Sonicate chromatin DNA to fragments ranging from 100 to 500 bp. 4. Immunoprecipitate chromatin DNA and antibody-associated beads by incubating them overnight at 4°C. The sequencing libraries of immunoprecipitated chromatin DNA were generated with varied index label by NEBNext® UltraTM DNA Library Prep Kit for Illumina (NEB, USA, cat. E7645L).
GEO
GSM8607954_r1