WGS of Streptomyces sp.: mangrove sediment
Genomic DNA was extracted using the TIANamp Bacteria DNA Kit (a spin column - based kit, Tiangen, China). Quality was assessed via spectrophotometer-measured A260/A280 (1.72.0) and A260/A230 (2.02.2) ratios, and integrity was checked by agarose gel electrophoresis. Sequencing libraries were prepared using the Illumina TruSeq DNA Sample Preparation Kit: genomic DNA was sonicated, followed by end-repair, A-tailing, and ligation of Illumina adapters, with magnetic bead purification and size selection.
SUB15204002
RUNSY159