GSM9836008: RNAseq HEK293 HEMK1 KO Repeat 1; Homo sapiens; RNA-Seq
For RNA isolation, 106 HEK293 wildtype or HEMK1 KO 1F8 cells were harvested by centrifugation at 300 rpm for 5 min. The total RNA was isolated using the QIAGEN RNeasy® Plus Mini Kit. RNA libraries were prepared using the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (NEB) according to the manufacturers protocol using 100 ng of total RNA per sample as input. Two biological replicates were processed. Sequencing libraries were prepared using the NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1).
GEO
GSM9836008_r1