GSM9769673: OCI-AML-3 DKO HiC; Homo sapiens; Hi-C
In-situ Hi-C was performed using the Arima-HiC+ Kit (Arima, A51008-ARI) following manufacturer protocol. In brief, one million cells were crosslinked with 2% formaldehyde as described above. Cell lysis, nuclear permeabilization, chromatin digestion and biotin ligation were all performed according to manufacturer protocol. DNA shearing was performed using Vibra-Cell (Sonics & Maerials, VCX750) in 32% Amplitude for in total 1 minute to produce ~400-600bp of fragments. Biotin enrichment was then performed using streptavidin-coated enrichment beads supplied in the Arima Hi-C kit according to the manufacturer's protocol. On-bead library preparation was performed using KAPA HyperPrep (Roche, 7962363001) following the adjusted protocol supplied by Arima.
GEO
GSM9769673_r1