HAdV-55 CQ2408
The DNA of sorted HAdV-55 was extracted from the cell line using the QIAamp MinElute Virus Spin Kit. The DNA samples were fragmented to 300-400 bp using a Covaris instrument for ultrasonic shearing. The library was then constructed through end repair, A-tailing, adapter ligation, and purification with Agencourt AMPure XP beads. The quality of the library was assessed using the Agilent 2100 Bioanalyzer.Single stranded circular DNA molecules replicate through rolling loops, forming a DNA nanosphere (DNB) containing over 300 copies. The obtained DNBs will be added to the mesh like pores on the chip using high-density DNA nanochip technology. Sequencing was performed using Combined Probe Anchored Aggregation (cPAS) technology.
SUB15198622
CQ2408