GSM8517872: HiC_SMC3KO_pDC_01; Mus musculus; Hi-C
Sorted cells (1-5x106 per sample) were cross-linked for 10 min in 1 ml of 2% methanol-free formaldehyde (Thermo Fisher Scientific) in PBS + 3% BSA. Crosslinking was reversed by addition of glycine (Thermo Fisher Scientific) to 0.125 M for 5 min, followed by incubation on ice for 15 min. Libraries were prepared using the Arima Hi-C kit according to manufacturer protocol.
GEO
GSM8517872_r1