GSM8520366: OH4; Homo sapiens; RNA-Seq
Total RNA was isolated from the cultured cells using a Rneasy mini kit (Qiagen) and eluted in 40 ul of nuclease-free water. The strand-specific RNA sequencing libraries for 8 individual samples were prepared using a TruSeq Stranded RNA Sample Prep Kit (Illumina). These samples represent four independent experiments for each of the two conditions examined. Libraries were pooled in equimolar concentration, and the pool was quantitated by quantitative PCR and sequenced on three lanes for 101 cycles on a HiSeq6000 (Illumina) using a HiSeq SBS sequencing kit version 2.
GEO
GSM8520366_r1