RNA-Seq of washed root of Typha latifolia after being supplied with OSPW at the time D6
Ribosomal RNA (rRNA) was depleted from 2 g of root RNA using the Pan-Prokaryote riboPOOL kit (siTOOLs Biotech) and streptavidin magnetic beads (New England Biolabs). Cleanup and concentration were performed using SPRIselect bead-based reagent (Beckman Coulter) and the RNA Clean & Concentrator-5 kit (Zymo Research). RNA was eluted in 10 l of water and quantified using the Qubit RNA High Sensitivity assay kit (Invitrogen). Libraries were prepared using the NEBNext Ultra II RNA Library Prep kit for Illumina (New England Biolabs), with 8 PCR cycles (94C for 8 minutes). NEBNext Multiplex Oligos were used for PCR enrichment. Libraries were assessed using the High Sensitivity DNA kit and 2100 Bioanalyzer (Agilent), and quantified with the Qubit dsDNA High Sensitivity assay kit (Invitrogen).
SUB15754645
NS.LH00487_0019.005.NEBNext_i715---NEBNext_i514.M11G-261-Root-Exp3-D6