ANA13B
For microbial DNA extraction, from the collected samples from each site, up to 5 L water samples were filtered onboard through 0.22 m Sterivex filters (Millipore, Billerica, MA, USA; SVGPL10RC). The microbial DNA extraction was extracted from each filter using DNeasy Blood and Tissue Kit (QIAGEN, Hilden, Germany) following the manufacturers protocol with some modifications. The V3-V4 variable region of the 16S rRNA gene was targeted for the screening of bacterial biodiversity. Amplicons were indexed using a Nextera XT Index Kit v2 (Illumina, San Diego, CA, USA) for multiplexing. The library was sequenced on Illumina MiSeq platform using 2300 bp paired-end protocol.
SUB15340031
MPA ST67 16S 1