GSM8659431: Il10-eGFP-Positive_1; Mus musculus; RNA-Seq
RNA was extracted from lysates using the PureLink RNA Micro Scale Kit (Invitrogen) according to the manufacturer's instructions. Library prep kit: Lexogen QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina. RNA input for prep: 5ng. The protocol for low input samples was followed, this includes alterations to incubation times. Unique Molecular Identifiers (UMIs) were used for the evaluation of input and PCR duplicates and to eliminate amplification bias. 22 cycles of PCR was performed. 4nM of pooled library was sequenced.
GEO
GSM8659431_r1