CutTag
Libraries were generated from 16 wildtype CD1-mice cortices at stage E15.5. Cortices were homogenized, dissociated and intranuclearly stained using a Tbr2 rabbit pAb (1:75; Abcam), followed by FACS sorting (Sakib et al., 2021; Ulmke et al., 2021). CUT&Tag libraries were prepared using the CUT&Tag-IT Assay Kit  Tissue (Active Motif), according to the manufacturers instructions. For each sample 125,000 bound nuclei were used. Primary antibodies included Pbx1 rabbit pAb (1:100, Cell Signaling) and Pbx3 rabbit pAb (1:100, Sigma). Normal rabbit IgG (1:100, Cell Signaling Technology) served as negative control, and H3K27me3 rabbit pAb (1:100, Abcam) as positive control. Four technical replicates were prepared for each target, and a paired-end Illumina sequencing of barcoded libraries was performed on an Illumina NextSeq1000 platform.
SUB15966099
CutTag-Pbx3-R1