btsl1_btsl2_Fe100_Shoot_R2
NEBNext Ultra RNA Library Prep Kit for IlluminaNEB, E7530and NEBNext Multiplex Oligos for Illumina (NEB, E7500). In briefly, the enriched mRNA was fragmented into approximately 200nt RNA inserts, which were used to synthesize the first-strand cDNA and the second cDNA. The double-stranded cDNA were performed end-repair/dA-tail and adaptor ligation. The suitable fragments were isolated by Agencourt AMPure XP beads (Beckman Coulter,  Inc.), and enriched by PCR amplification. Finally, the constructed cDNA libraries were sequenced on an Illumina sequencing platform.
SUB14722862
T35