High-Fidelity reads of Pinctada maxima
Select high-quality DNA samples that pass inspection (with the main band >30kb); randomly fragment the DNA into pieces (15-18kb) using a Covaris sonicator; enrich and purify large DNA fragments with magnetic beads; perform damage repair and end repair on the fragmented DNA; attach stem-loop sequencing adapters to both ends of the DNA fragments and remove failed connection fragments with exonucleases.
SUB14943271
SMRTbell