RNA-seq on Zfy Knock-out male germ cells
RNA-seq on Zfy Knock-out male germ cells
Primary spermatocytes (SC1), secondary spermatocytes (SC2), and round spermatid (RS) cells were isolated from wild type (XY), Zfy1 KO, Zfy2 KO, and Zfy DKO mice via germ cell isolation and fluorescence-activated cell sorting (FACS). RNA was extracted from each SC1, SC2, and RS cell fraction using the RNeasy Micro Kit (Qiagen, Hilden, Germany). The cDNA libraries were prepared using the NEBNextÂ® UltraTM II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) before quality assessment with the Agilent Bioanalyzer.
European Bioinformatics Institute
E-MTAB-14570:Sample 20_p