GSM9324018: 6_5B4-HepG2--DMSO-control_3; Homo sapiens; RNA-Seq
1x10e6 cells were lysed in Trizol (Sigma) and RNA was extracted according to protocol using Direct-zol RNA mini prep kit (Zymo Research), in-column DNAseI treatment was performed during the extraction. Quantity was assessed using Qubit (Thermofisher) and quality was assessed using RNA Tapestation (Agilent). 500ng of input total RNA was used to make Quantseq libraries for Differential gene expression analysis (Lexogen) according to protocol. 13 PCR cycles were used to amplfy and index cDNA into final library. Quantity and library size was assessed using Qubit and DNA tapestation, respectively. Equimolar quantities of each library were pooled into one sample and sequenced on the Nextseq 2000 (Illumina), single read length 76bp and at least 10M reads/sample.
GEO
GSM9324018_r1