GSM8899925: Liver_MD_5; Mus musculus; RNA-Seq
RNA was extracted from four randomly selected (using Randomice tool) snap-frozen mouse liver tissues (~ 50 mg) using Trizol. Overall quality of RNA was verified using Agilent's 2100 Bioanalyzer (Agilent) Standard RNA-seq libraries were constructed using NEBNext poly(A) mRNA isolation kit (New England Biolabs, Ipswich, MA) followed directly by IDT's XGen Broad Range RNA Library Prep Kit (Integrated DNA technologies, Coralville, IA) and the established protocols. The library construction was done using 500 ng of RNA. Each of the libraries was indexed during library construction to multiplex the sequencing. Libraries were quantified using Invitrogen's Qubit 4 fluorometer (Thermofisher Scientific) and checked for size and quality using Agilent's 2100 Bioanalyzer (Agilent).
GEO
GSM8899925_r1