GSM8971953: E2-poly A; Mus musculus; RNA-Seq
RNA was extracted by using the the Quick-RNA Miniprep Plus kit from Zymo Research (ZYMO Research) cDNA libraries were generated using the NEBNext® UltraExpress™ RNA Library Prep Kit for Illumina® (New England Biolabs), designed to convert 25–250 ng total RNA into high-quality sequencing libraries with minimal hands-on time. The workflow included mRNA enrichment, fragmentation, random-primed cDNA synthesis, end-repair, adapter ligation, and PCR amplification with indexed primers.
GEO
GSM8971953_r1