Y2_3 (CRX1847439; CRA027638)
Total RNA extraction from each sample was performed using a TRIzol reagent kit (Invitrogen, Carlsbad, CA, USA). RNA quality was assessed using a 2100 Bioanalyzer (Agilent Technologies) and confirmed by RNase-free agarose gel electrophoresis. mRNA was enriched using Oligo (dT) beads and divided into short fragments with fragmentation buffer. The enriched mRNA was reverse transcribed into cDNA using a reverse transcription kit (NEB #7530, New England Biolabs, Ipswich, MA, USA).
Hebei Agricultural University
DRX796635
NGDC
CRX1847439