Partial genome sequencing of Brachyplatystoma rousseauxii
The SNPs development included construction of sequence libraries which were prepared using a variant of the ddRAD protocol [39] adapted for IonTorrent PGM plataform (Life TechnologiesTM) and described in Martnez et al. (2016) [40], with Csp6I and SdaI restriction enzymes (Thermo Scientific). For more details, see the complete protocol on GitHub (https://github.com/legalLab).
SUB15549244
17BfCAR