GSM8553238: Dorsal root ganglia, vehicle ip administration in the E2(-) group, rep1; Rattus norvegicus; RNA-Seq
Total RNA was extracted from the DRG using the RNeasyⓇ Plus Universal Mini Kit (QIAGEN, Tokyo, Japan), according to the manufacturer's protocol. 1 μg of total RNA was used for the construction of sequencing library. cDNA libraries (150-bp × 2 paired-end reads) were prepared using the NEBNextⓇ Poly(A) mRNA Magnetic Isolation Module kit (Cat No. E7490) and NEBNEXTⓇ UltraTMII Directional RNA Library Prep Kit (Cat No. E7760) (New England Biolabs, MA, USA).
GEO
GSM8553238_r1