GSM9212082: adenoma from BrafV637E mutant mouse; Mus musculus; RNA-Seq
RNA was isolated from intestinal epithelial cells and tumours using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen) and its concentration and quality assessed using the TapeStation D1K TapeScreen assay (Agilent). cDNA libraries were prepared using the TruSeq RNA Sample Prep Kit (v2) (Illumina Inc, San Diego, USA) or the stranded RNA library preparation kit with rRNA depletion (Illumina) and sequenced on a NovaSeq 6000 sequencer (100 paired end single reads).
GEO
GSM9212082_r1