GSM9326279: Kidney Slc7a9-/- male L-Ergothioneine treated 4; Mus musculus; RNA-Seq
Total RNA was extracted from frozen kidney tissue using TRIzol Reagent (Ambion®, Thermo Fisher Scientific, MA, USA) and a Polytron PT-1200 homogenizer (Kinematica, Switzerland) following the protocol provided by the Direct-zol™ RNA MiniPrep Plus Kit (Zymo Research, CA, USA). RNA libraries were prepared for sequencing using the kits Illumina® Ribo-Zero plus (20036696), Illumina® Stranded mRNA Prep, Ligation (20040534) and IDT® for Illumina® RNA UD Indexes Set A, Ligation (20040553), following manufacturer's instructions and 10 cycles of amplification.
GEO
GSM9326279_r1