GSM8766968: Sample_HP2_igG; Mus musculus; OTHER
For the CUT&Tag experiments, nuclei isolation was performed according to the published protocol by using a minimum of 105 nuclei. Nuclei were resuspended in 200 μL antibody buffer containing 1 μg FOXO1 antibody or IgG and then incubated at 4ºC overnight. Qubit Flex was used for initial quantitation after the library was constructed, and the library size range was checked using an Agilent 4200 Tapestation to ensure the inserted fragment size. After qualified library inspection, different libraries were pooled according to the requirements of effective concentration and target data volume.
GEO
GSM8766968_r1