STEREOSEQ_Spatial_Yersinia_infection_intestine
All Library preparation steps were performed with Complete Genomics. Spatial transcriptomics library generated using the STOmics Stereo-seq Omni protocol on a formalin-fixed paraffin-embedded (FFPE) mouse small intestine (Swiss roll) section mounted on Chip N (ID: Y01679L2). Total RNA was captured via total RNA random priming, reverse transcribed in situ to attach spatial Coordinate Identifiers (CIDs) and Molecular Identifiers (MIDs), and sequenced on a DNBSEQ platform in a paired-end configuration (PE75_25+59). Read 1 contains the 25 bp spatial barcode and MID, while Read 2 contains the 59 bp organism transcript insert mapped to a custom GRCm39 reference genome. The tissue mask file (Y01679L2.barcodeToPos.h5) and custome genome are available in GEO.
SUB15822377
Yp1_FFPE_Intestine