RNA-seq of DOA9WT: free living growth
To determine gene expression in Bradyrhizobium sp. strain DOA9 wild-type (DOA9WT) and its derivative mutants under free-living conditions, 1% (v/v) prepared cell suspensions of each strain were inoculated into 150-mL glass bottles containing 50 mL of BNM-B broth and incubated statically at 30C for 7 days. Cells were harvested by centrifugation at 4,000  g and 4C for 10 minutes, and total RNA was extracted using the Flavogen RNA Purify Kit (Qiagen) followed by DNase I treatment (Thermo Scientific) to remove genomic DNA contamination. RNA samples were submitted to GENEWIZ Biotechnology Co., Ltd. (China) for RNA sequencing.
SUB15027507
WT