GSM9003820: Monocyte short-read RNA-Seq, 3h LPS in vitro activation, healthy volunteer 7; Homo sapiens; RNA-Seq
Monocytes were lysed in TRIzol LS reagent (Thermo Fisher) directly or after LPS activation. RNA was isolated using the Dynabead mRNA Purification Kit (Thermo Fisher) according to the manufacturer's protocol. For short-read RNA-Seq, the concentration of RNA was quantified using a Qubit RNA HS assay and Qubit fluorometer (Thermo Fisher). For Native RNA-Seq, 1 μM mRNA per sample was loaded on MinION flowcells and ran for approximately two hours at the Utrecht Sequencing Facility. For short-read RNA-Seq, polyadenylated messenger RNA was isolated using Poly(A) beads (NEXTflex), and sequencing libraries were made using the Rapid Directional RNA-seq kit (NEXTflex).
GEO
GSM9003820_r1