D2_PBS2 (CRX1934902)
Total RNA quality was first assessed using a NanoDrop 2000 spectrophotometer to determine purity and concentration, and an Agilent 2100 Bioanalyzer to evaluate RNA integrity.  Following quality confirmation, mRNA was enriched using oligo(dT) magnetic beads, fragmented with a fragmentation buffer, and reverse transcribed into cDNA using random hexamer primers.  Second-strand synthesis was then performed, and the resulting cDNA fragments were subjected to end-repair, A-tailing, and adapter ligation.  Size selection of the ligated fragments was carried out using AMPure XP beads, and PCR amplification was conducted to generate the final libraries.
NGDC
CRX1934902
The Second Affiliated Hospital of Xi 'an Jiaotong University
DRX1003020