GSM8798288: KYSE150-3-IgG; Homo sapiens; OTHER
Cells are resuspended in tagmentation buffer (10mM MgCl2 in dig-300 buffer) and incubated at 37°C for 1 hour to allow for the tagmentation reaction to occur. DNA Purification: Using Tagment DNA Extract Beads to purify the DNA after the tagmentation reaction. Primer Mixing: Mixing the purified DNA with universal and barcoded primers. PCR Amplification: Amplifying the DNA using NEBNext HiFi 2× PCR Master mix and specific cycling conditions. Post-PCR Clean-up: Cleaning up the amplified DNA using XP beads to obtain the final library.
GEO
GSM8798288_r1