16S-Seq of tumor core tissue-1
In the first step, extract the total DNA from the tumor core sample. Then, synthesize specific primers with barcodes for the designated sequencing regions (five regions of the 16S: V2, V3, V5, V6, V8). Perform multiplex PCR amplification, recover the PCR products, and construct a library using Illumina PE300/PE250 for sequencing on the instrument.
SUB14766018
TC-1