RNA-seq of fecal culture:in vitro 24h
The total DNA from various subgroups of samples was collected through a MicroElute Genomic DNA Kit. The diluted DNA was amplified by the PCR system with the 20 L reaction system using TransStart FastPfu DNA Polymerase (TransGen AP221-02). To analyze bacterial diversity, the universal primers 338F (5-ACTCCTACGGGAGGCAGCAG-3) and 806R (5-GGACTACHVGGGTWTCTAAT-3) were employed to amplify the 16S rRNA gene in the variable area of bacteria V3-V4. Sequencing libraries were created by purifying, calculating, and homogenizing the amplified products. The Illumina HiSeq 2500 platform was used to sequence the qualified library.
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