GSM9324717: Winter - 2; Microtus ochrogaster; RNA-Seq
Total mRNA was isolated from frozen tissues using miRNeasy mini-Kit (Qiagen, France), with DNAse treatment. The quality and quantity of total RNAs were determined using 4150 TapeStation and RNA ScreenTapes (Agilent Technologies). cDNA libraries were prepared using KAPA mRNA HyperPrep Kit (Roche Diagnostics). This kit captures messenger RNA using magnetic oligo-dT beads and it fragments the material using heat and magnesium. Then first strand cDNA was synthetized using random priming and Illumina adapters were added by ligation. Library preparation was performed according to supplier recommendations and as previously described (Khaled et al. 2021), using 1 µg of total RNA input to ensure high diversity.
GEO
GSM9324717_r1