RAT-E3 (CRX1929800; CRA029274)
Eukaryotic mRNA was enriched by Oligo ( dT ) magnetic beads, and the mRNA was randomly interrupted by Fragmentation Buffer.Then the first cDNA strand and the second strand were synthesized using mRNA as a template, and the cDNA was purified. The purified double-stranded cDNA was then subjected to terminal repair, A-tail was added, and the sequencing adapter was ligated. Then AMPure XP beads were used to select the fragment size. Finally, the cDNA library was obtained by PCR enrichment.
NGDC
CRX1929800
Zunyi Medical University
DRX812620