RNA-Seq of formation water-enriched inoculum at high pressure
Total RNA was extracted from incubation samples using an RNA extraction kit (Majorbio, shanghai, China) according to the manufacturers protocols. RNA concentration was measured using a Qubit V.4.0 Fluorimeter (Thermo Fisher Scientific, USA) and the quality was evaluated using a bioanalyzer (JY600C, Beijing JUNYI Electrophoresis Co., Ltd., China). rRNA in samples were removed using Ribo-Zero rRNA Removal Kits (Lexogen, USA) as per the manufacturers instructions. Enriched mRNAs were used to construct an mRNA-enriched metatranscriptomic library using a TruSeq RNA Sample Prep Kit (Illumina, USA). The metatranscriptomic library was sequenced using an Illumina HiSeq 4000 (paired-end; insert size, 300 bp; read length, 150 bp).
SUB15141860
RNA-HP-f