GSM9083197: MDA-MB-231 cells, FLAG-tagged RBMS3, biol rep 1; Homo sapiens; RIP-Seq
First, cells were irradiated using a 254nm UV-crosslinker at 400mJ/cm2. Cells were then lysed on ice before Immunoprecipitaiton of RNA-RBP complexes with FLAG Magnetic Agarose Beads (Pierce, Anti-DYKDDDDK). Library preparation was performed as detailed in: Integrative analysis of mRNA stability regulation uncovers a metastasis-suppressive program in breast cancer. Heather Karner, Tabea Mittmann, Vicky W. Chen, Ashir A. Borah, Andreas Langen, Hassan Yousefi, Lisa Fish, Balyn W. Zaro, Albertas Navickas, Hani Goodarzi bioRxiv 2025.06.06.658309; doi: https://doi.org/10.1101/2025.06.06.658309
GEO
GSM9083197_r1