S2, duplicate 1
The library was constructed from the fragmented DNA using the NEBNext enzymatic methyl-seq library kit (#103496, Twist Bioscience) and quantified with the Qubit 1xds DNA HS assay kit(Q33230, Thermo Fisher Scientific). The library sizes were evaluated with a high-sensitivity D1000 screen tape assay (5067-5584, Agilent Technologies). An equal amount of DNA from 8 libraries was pooled for target enrichment with the Twist human methylome panel (105517, Twist Bioscience) by following the Twist targeted methylation sequencing protocol. The final enriched enzyme-converted libraries were sent to Hartwell Center at St. Jude Childrens Research Hospital for 150 bp paired-end sequencing on NovaSeq6000 platform.
SUB14913787
S10