GSM9178431: liver, Rougan Keli dosing group, 3; Mus musculus; RNA-Seq
At the end of the animal experiment,  the liver was taken, weighed, and an appropriate amount of liver tissue was placed in 4% formaldehyde, 2.5% glutaraldehyde fixative, or stored in a refrigerator at -80°C, respectively. Then cells were loaded into microfluidic chip of Chip A Single Cell Kit v2.1 (MobiDrop (Zhejiang) Co., Ltd., cat. no. S050100301) to generate droplets with MobiNova-100(MobiDrop(Zhejiang) Co., Ltd., cat. no.A1A40001). Each cell was involved into a droplet which contained a gel bead linked with up to millions oligos (cell unique barcode). After encapsulation, droplets suffer light cut by MobiNovaSP-100(MobiDrop (Zhejiang) Co., Ltd., cat. no.A2A40001) while oligos diffuse into reaction mix. The mRNAs were captured by cell barcodes with cDNA amplification in droplets. Following reverse transcription, cDNAs with barcodes were amplified, and a library was constructed using the High Throughput Single-Cell 3' Transcriptome Kit v2.1 (MobiDrop (Zhejiang) Co., Ltd., cat. no. S050200301) and the 3' Dual Index Kit(MobiDrop (Zhejiang) Co., Ltd., cat. no.S050300301)
GEO
GSM9178431_r1