snRNA-seq of C. elegans: Adult Male Nuclei
Single nuclei suspension samples were loaded to the 10X Genomics Chromium X system using the Single Cell 3 v3.1 Reagent Kits (10X Genomics Inc., CA) to generate and amplify cDNA. Up to 30,000 nuclei were loaded per 10X sample. The amplified cDNA samples were purified with Ampure XP magnetic beads (Beckman Coulter, CA), quantified by Qubit fluorometer (Invitrogen, CA), and examined on Bioanalyzer with High Sensitivity DNA chips (Agilent, CA) for size distribution. Illumina sequencing libraries were generated from the amplified cDNA samples using the Illumina Tagment DNA Enzyme and Buffer kit (Illumina, CA). These libraries were examined by Qubit and Bioanalyzer, then pooled at equal molar amount and sequenced on Illumina NovaSeq 6000 S Prime flowcells as 28+94 nt pair-end reads following the standard protocol.  Raw sequencing reads were filtered by Illumina NovaSeq Control Software and only the Pass-Filter (PF) reads were used for further analysis.
SUB15411018
Male Rep 5