genome sequencing of apeLg1
The genomic DNA was extracted from silica-gel-dried leaves using the sorbitol extraction method and then purified using 0.4X AMPure XP (Beckman Coulter Inc., Brea, California, USA). Shotgun sequencing followed the genome sequencing protocol LITE of Perez-Sepulveda et al. (2021), starting with 1ng of DNA input amount. DNA was tagmented using Nextera chemistry (Illumina), followed by the ligation of P5 and P7 adapters with unique barcodes, library purification and quality control on LabChip (Revvity).  Libraries were pooled equimolarly based on the concentration of final size-selected fragments (400-600 bp), and the pools were size-selected on Pippin prep (Sage Science). The libraries were sequenced in 300 cycles (2  150 bp paired-end reads) on the Illumina NovaSeq platform.
SUB15669334
apeLg1