ND2_FKDL202584358-1a (CRX759968; CRA012133)
5X104 cells for each sample were isolated for ATAC library preparation using TruePrepTM DNA Library Prep Kit V2 for Illumina (Vazyme) and TruePrepTM Index Kit V2 for Illumina (Vazyme).Briefly, nuclei were extracted from samples, and the nuclei pellet was resuspended in the Tn5 transposase reaction mix. The transposition reaction was incubated at 37degreeC for 30 min. Adapter1 and Adapter 2 were added after transposition, PCR was then performed to  amplify the library. After the PCR reaction, libraries were purified with the AMPure beads and library quality was assessed with Qubit.
NGDC
CRX759968
Sun Yat-sen University
DRX800051