GSM9084228: H727, lung carcinoid cells, active YAP overexpression, biol rep 1; Homo sapiens; RNA-Seq
Total RNA was isolated from cells using TRIzol Reagent according to the manufacturer's instructions. Total RNA was quality controlled through UV spectrophotometry using a SmartSpecTM Plus Spectrophotometer and visualization through 1% agarose gel electrophoresis. cDNA libraries were prepared using the NEBNext Ultra RNA Library Prep Kit and the NEBNext rRNA Depletion Kit according to the manufacturer's instructions; RNA fragmentation was performed for 10 min. Libraries were sequenced on an Illumina NovaSeq 6000 platform.
GEO
GSM9084228_r1