GSM9501427: scRNA of spleen MASH treatment; Mus musculus; RNA-Seq
Cells were resuspended in PBS with 0.04% BSA at a concentration of 700-1200 cells/µl and more than 85% viability. The single cell capture and library construction were performed by following the Chromium Next GEM Single cell 5ʹ Reagent Kits v2 protocol (CG000331). The single cells were loaded onto Chromium Next GEM Chip K with partitioning oil and barcoded singe cell gel beads. The barcoded and full-length cDNA is produced after incubation of the gel beads-in-emulsion (GEMs) and amplified via PCR for library construction. The library preparation is performed by following the protocol of Chromium Next GEM Single Cell 5ʹ GEM, Library & Gel Bead Kit v2 (PN-1000267). The quality of the final libraries was examined using Agilent High Sensitive DNA Kit (#5067-4626) by Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, USA). The qualified libraries were sequenced using the paired-end sequencing on an Illumina Novaseq System (Illumina, Inc., USA).
GEO
GSM9501427_r1