GSM8835012: Hep3Bcells, shControl-1; Homo sapiens; RNA-Seq
RNA was extracted from Hep3B cells using a standard extraction method. The RNA samples were then subjected to strict quality control,  primarily using the Agilent 2100 Bioanalyzer to accurately assess RNA integrity. mRNA was enriched from total RNA using Oligo dT magnetic beads. After fragmentation,  the first-strand cDNA was synthesized using random hexamer primers,  followed by the synthesis of the second-strand cDNA. After end repair,  A-tailing,  adapter ligation,  fragment selection,  amplification,  and purification,  the library was ready for use.
GEO
GSM8835012_r1