GSM8517985: ChIP_pDC_CTCF; Mus musculus; ChIP-Seq
Sort-purified cells (~3.5-4.5x106) were cross-linked with 1% methanol-free formaldehyde in PBS for 8 min followed by quenching with 0.125 M glycine for 5 min. Cells were lysed and nuclei isolated using the Covaris TruCHIP Kit, and chromatin was sheared using a Covaris M220 ultrasonicator. ChIP was performed using 4 μg of rabbit anti-CTCF polyclonal antibody (Millipore) or rabbit anti-mouse/human Rad21 polyclonal antibody (Abcam) and Dynabeads Protein A (Thermo Fisher Scientific). Libraries were prepared using the KAPA HyperPrep Kit (Roche). As a control, libraries were also prepared from non-immunoprecipitated “input” DNA from each cell type.
GEO
GSM8517985_r1