GSM9693424: SIRT1intKO liver untreated biological replicate 1; Mus musculus; RNA-Seq
Total RNA was isolated from liver tissue using QIAzol Lysis Reagent (Qiagen, 79306) according to the manufacturer's instructions. RNA quantity and quality were assessed prior to library preparation. Stranded mRNA libraries were prepared by Macrogen Ltd. using the TruSeq Stranded mRNA Library Prep Kit (Illumina), following the manufacturer's protocol. Libraries were sequenced on the Illumina NovaSeq X platform to generate 100 bp paired-end reads, with an approximate depth of 40 million paired-end reads per sample.
GEO
GSM9693424_r1