Brassinosteroids Enhance Barley De-Acclimation Tolerance via Modulation of Chloroplast- Associated Gene Expression
Brassinosteroids Enhance Barley De-Acclimation Tolerance via Modulation of Chloroplast- Associated Gene Expression
Leaves from each mutant and Bowman cultivar were sampled after cold acclimation (CA) and after 24 h after de-acclimation (DA) in three biological replicates (leaves from three different plants). The leaves were immediately frozen in liquid nitrogen after sampling and stored at −80°C until use. Each sample was collected from the middle part of the youngest fully developed leaf and weighted 0.03–0.05 g. Plants were sown in pots (30 cm wide and 15 cm long) and grown for 3 weeks in a greenhouse at 18 °C (day/night) in daylight (the experiment was conducted in November in Krakow, Poland, 50°03' N, 19°55' E). Next, the plants were placed in a growth chamber for cold acclimation at a temperature of 2 °C/2 °C (day/night), with a photoperiod of 8 h/16 h (day/night) and 250 umol m-2 s-1 PPFD for 3 weeks. After cold acclimation, half of plants was de-acclimated at 12 °C/10 °C (day/night) 8 h/16 h (day/night) and 250 umol m-2 s-1 PPFD for 24 hours. Total RNA was isolated from 18 leaf samples using the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany). mRNA was isolated using oligo(dT)-attached magnetic beads, and cDNA libraries were constructed, converted into single-stranded circular DNA (ssCir DNA)
European Bioinformatics Institute
E-MTAB-15311:Sample 16_p