GSM8864045: iKRAS, 16 weeks ON dox - lung2; Mus musculus; RNA-Seq
Lungs were first perfused with PBS before being harvested from the mouse. Lung tissue was then mechanically minced and incubated with Collagenase P (Sigma-Aldrich, #11249002001) for 30 minutes at 37°C under constant shaking. All tissue was then subject to serial filtration through 500-, 100-, and 40-um mesh. To remove red blood cells, single cells were then subject to ACK Lysis buffer (Gibco, A1049201) until most red blood cells were lysed. We enriched for live cells using the MACS Dead Cell Removal Kit (Miltenyi Biotec, #130-090-101). Bone marrow was taken from both femurs by flushing with PBS. The resulting bone marrow suspension was subject to ACK lysis buffer (Gibco, A1049201) to remove red blood cells and then run through a 70-um mesh. Samples were run using 50-cycle, paired-end reads on the Illumina NovaSeq 6000 (Illumina, San Diego, CA) to a depth of 100,000 reads.
GEO
GSM8864045_r1