RNA-Seq of mus musculus: female intestine
Total RNA was extracted from frozen intestinal tissues and purified using the miRNeasy Tissue/cells Advanced Mini Kit (Qiagen). Following quality assessment using the Agilent Bioanalyzer 2100, cDNA libraries for sequencing were constructed using the Ultra II Directional RNA Library Prep Kit for Illumina with Poly(A) mRNA Magnetic Isolation Module (NEB). The prepared library was then sequenced on the illumina NovaSeq 6000 and 20-30 million paired-end reads were generated.
SUB15468566
KaisoTg 5