Amplicon sequencing of Influenza A barcoded virus from nasal wash of guinea pig e02-gp42 on day 3
Viral RNA extraction was performed using the NucleoMag RNA extraction kit, followed by a one-step RT-PCR reaction using the SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity DNA Polymerase and primers targeting the barcoded region of our virus (forward: 5-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGACCTTCTTCTTGACTCAAGGG-3, reverse: 5-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGCAAGCGACTGACTCAAATCTTGA-3). PCR products were purified using a column-based kit, then quantified using the Qubit dsDNA quantification kit prior to paired end sequencing.
SUB15923013
e02-gp42_D3-NW