Whole metagenome of bacterial biofilm
The genomic DNA sample was fragmented into short fragments. These DNAfragmentswere then end-polished, A-tailed, and ligated with full-length adapters for Illumina sequencingbefore further size selection. PCR amplification was then conducted unless specified as PCRfree. Purification was then conducted through the AMPure XP system (Beverly). The resultinglibrary was assessed on the Agilent Fragment Analyzer System (Agilent) and quantifiedto1.5nM through Qubit (Thermo Fisher Scientific) and qPCR.
SUB15094314
LPR