osprr37-1 (CRX2114079)
Total RNA was extracted from tissue samples, and A-T base pairing was performed using magnetic beads with Oligo ( dT ) and ployA.Fragmentation buffer was added, and appropriate conditions were selected to randomly cleave mRNA into small fragments of about 300 bp. Under the action of reverse transcriptase, one-strand cDNA was synthesized by using random primers with mRNA as template, and then two-strand synthesis was carried out to form a stable double-strand structure. End Repair Mix was added to fill it into a flat end, and then an A base was added to the 3 ' end to facilitate the subsequent addition of the adapter sequence. The products after the adapter was ligated were purified and the fragments were sorted. The sorted products were subjected to PCR amplification, and the final library was purified.
NGDC
CRX2114079
Sichuan Agricultural University
DRX1009503