GSM9066359: rpl-36A(P55Q)/mIn1 heterozygotes, -CHX, 2-day, 23˚C, L4, Rep1 (50-80 nt); Caenorhabditis elegans; OTHER
Worm lysate was loaded onto 34% sucrose made with the lysis buffer, and spun at 70,000 rpm using TLA 100.3 rotor (Beckman Coulter) for 4 hours at 4 ℃. The resulting pellet was solubilized in 1 ml of TRIzol to extract RNA. Resulting RNA was resolved on 10% Acrylamide TBE-urea gel (ThermoFisher Scientific), fragments between 50-80 nucleotides were cut for RNA extraction. Disome-seq libraries were prepared using D-Plex Small RNA-seq Kit (diagenode) following manufacturer's protocols.
GEO
GSM9066359_r1