RNAseq of pooled ephyrae post-amputation: low-food diet + penicillin
mRNA was isolated from ~750 ng of total RNA through poly-A selection. RNA-seq libraries were constructed using the NEBNext Ultra II RNA Library Prep Kit for Illumina following the manufacturers protocols. Briefly, mRNA was fragmented to an average size of 350 nt. cDNA was then synthesized using random primers followed by second strand synthesis. Resulting DNA fragments were end-repaired, dA tailed, and ligated to adaptors. Following ligation DNA fragments were size selected to generate fragment sizes between 400-500 bp. Adaptor-ligated DNA was PCR amplified.
SUB15230398
Pen-23910_S15