CD8 T cells single cell transcriptomic analysis from human blood
CD8 T cells single cell transcriptomic analysis from human blood
Freshly sorted CD8+ T lymphocytes of four healthy volunteers were labelled with anti-human Hashtag antibodies TotalSeq-C (Biolegend, San Diego, CA) and were pooled together.  Disintegration of cells or tissues (cell lysis), inactivation of intracellular nucleases, and separation of nucleic acids from the other cell components Single cell libraries were performed according to Chromium Next GEM Single Cell 5' Reagent Kits v2 protocol (10X genomics, San Francisco, CA). 
European Bioinformatics Institute
E-MTAB-15460_2:Sample 4_GEX_p