scRNA-seq of cDNA from MDA-MB-231 with RBP perturbations using long-read transcriptomics
A total of 300 ng of cDNA gene expression amplicon library was prepared for nanopore cDNA sequencing. No fragmentation was performed and full-length cDNA was quantified and sized using Qubit fluorometer and Agilent Bioanalyzer, respectively. The sequencing library was prepared using Oxford Nanopore Technologies SQK-LSK110 library preparation protocol and was sequenced on a PromethION FLO-PRO002 flowcell. Basecalling was performed with MinKNOW and Guppy v5.1.13 using the dna_r9.4.1_450bps_HAC model, yielding a total of 88 million cDNA reads.
SUB15785275
CRISPore-seq_long-read