WGS of Caenorhabditis elegans: neurodegenerative mutants
To grow sufficient mutant worms for genomic DNA extraction, 70 worms of each mutant line were placed onto five 10 cm NGM plates seeded with E. coli OP50 and allowed to lay eggs for 24 hours before removal. The offspring were grown until they reached the adult stage. Genomic DNA was extracted using the Gentra PureGene Kit (QIAGEN Inc, Germantown, MD) following instructions from the manufacturer. DNA was then sent to Novogene Corporation, Inc (Sacramento, CA) for whole genome sequencing. An Illumina NovaSeq 6000 platform was used to obtain short reads of all samples.
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