16S amplicon on soil DNA samples from pots with C. bonarienzis legacy cultivated with C. intybus
The library was prepared using the 16S Barcoding Kit 112 (Oxford Nanopore Technologies), where the full-length 16S rRNA gene was amplified via PCR using barcoded primers (27F/1492R), followed by purification with Agencourt AMPure XP beads. Equimolar amounts of barcoded amplicons (100 ng per sample) were pooled and sequenced on a MinION R9.4.1 SpotON FlowCell, using MinKNOW v3.6.5 for run control.
SUB15049396
ONT_barcode130