GSM8607944: PD; Mus musculus; RNA-Seq
), mice were injected IP with 5ml PBS and the peritoneal wash (corresponding to the OC TME) collected from 4 mice/group. Recovered cells were washed in PBS and immediately stained with Zombie NIR, prepared in PBS for sorting of viable cells. Stained cells were sorted using a BD FACSAria II, collecting 30,000 live cells per mouse, which were then pooled within cohorts (120,000 live cells/group). For later time points, mice receiving  FR-B T cell + anti-PD-1 treated mice were categorized as having Progressive Disease (PD; defined as animals with ascites accumulation and abdominal circumference ≥ 8.0 cm) or Durable Response (DR; defined as mice having no ascites accumulation) on Day 38. Ascites and/or peritoneal washes were collected from PD and DR animals (n=4/group), subjected to ACK lysis to remove red blood cells, counted and pooled equally within each cohort (50,000 cells/mouse) to reach 200,000 total live cells/group.  Similarly, ascites or peritoneal wash from mice treated with FR-B T cells + aPD1 or FR-B T cells+ aPD1+ Ad-OVA Vax were collected 14 days post vaccination (day 28) were collected from 4 mice/group, subjected to ACK lysis, counted, and equally pooled to reach 120,000 live cells/group. Immediately following preparation, cells were provided to the Roswell Park Genomics Shared Resource (GSR) for further processing and single cell sequencing (10x Genomics). Single Cell 5' Reagent Kits v2 (Dual Index) 10x Genomics 5'GEX
GEO
GSM8607944_r1