GSM8518515: ChIPseq-SET11-SDG2(H1866K)cd-H3K4me3-rep1; Arabidopsis thaliana; ChIP-Seq
Total RNA was extracted from 4-5 week old leaf tissue from each sample by using Zymo RNA extraction kit. ChIP-seq libraries were prepared by using the Ovation Ultra Low System V2 kit (NuGEN). Genomic DNA was extracted by using the CTAB-based DNA extraction. 1 ug RNA was used to prepare the RNA-seq library by following Illumina kit. Three grams of Arabidopsis leaf tissue (4-5 week old) of each sample were used for ChIP experiment. 10 minutes cross-link with 1% formaldehyde. The chromatin was sheared with Biorupter with 26 cycles. 500 ng DNA of each sample was sheared at 4°C for 2 min by using Covaris, and the WGBS library construction used Kapa DNA Hyper Kit (Roche) and TruSeq DNA UD indexes for Illumina (Illumina)
GEO
GSM8518515_r1