RNA-seq of human: child female feces
The child's guardian collected freshly produced, urine-free fecal samples at home and transported them to the hospital within one hour, maintaining low temperatures using an ice pack. Upon arrival, the samples were immediately flash-frozen in liquid nitrogen and subsequently stored at -80 C for long-term preservation. Genomic DNA was extracted from the fecal samples using the CTAB/SDS method. The quality and concentration of the extracted DNA were assessed via agarose gel electrophoresis. The hypervariable V3-V4 region of the 55S rRNA gene was amplified through PCR, and the resulting amplicons were purified to construct sequencing libraries. Finally, the libraries were sequenced on the Illumina MiSeq platform to generate high-throughput sequencing data for analysis.
SUB15340673
OC-8