Nagtive Control for TRNAU1AP and PRPF39
Negative control for knockouts of TRNAU1AP and PRPF39. Cell populations of lentivirally-transduced cells expressing Cas9 and gene-specific sgRNAs (pAVA3773, Negative control sgRNA) were FACS-isolated. Extracted RNA was diluted to 50ng/ul and sent for cDNA library construction (without the use of poly(A) selection) and paired-end sequencing (100-bp paired-end reads) following the manufacturer's protocols (BGI-America, San Jose, CA). 60 millions clean reads received for each sample
SUB15720918
MMDM13