Paired-end RNA-seq of porcine metaphase II (MII) oocyte DK intervention group replicate 3
Single-cell RNA-seq was performed on porcine metaphase II (MII) oocytes using the Smart-seq2 protocol. Individual oocytes were collected from in vitro matured cumulus-oocyte complexes (COCs) of prepubertal crossbred gilts. Cumulus cells were completely removed prior to RNA extraction. Total RNA was extracted from single oocytes, mRNA was enriched by Oligo-dT beads, and cDNA was synthesized and amplified using standard Smart-seq2 method. Paired-end sequencing libraries were constructed with 300 bp insert size and sequenced on the Illumina NovaSeq 6000 platform with 150 bp read length.
SUB16273857
DK3_lib