PACBIO data from Andesiops torrens
Genomic DNA was extracted from whole-body tissue using the E.Z.N.A. Insect DNA kit (Omega Bio-Tek), with protocol modifications to reduce the high protein and RNA content, involving extended incubation with the CTL buffer (2h at 37C instead of 30min at 60C) and prolonged RNAse treatment (1h at 37C instead of 10min at 70C). For short-read sequencing, DNA was extracted from a single individual, while long-read sequencing used pooled DNA from 14 individuals to reach required DNA amounts.For PacBio Sequel sequencing, 6 g of genomic DNA was employed to construct a 10 kb library using PacBio SMRTbell Express Template Prep Kit 2.0. The library average insert size was 19,791 bp. SMRTbell templates were annealed using Sequel II Bind Kit 2.0 and Internal Control 1.0, and then sequencing was performed using the Sequel II Sequencing Kit 2.0 and cells (8M Tray, with 15-hour movies).
SUB15790807
Andesiops_torrens_PACBIO