Metagenomic analysis of decomposed leaf litter: Degraded Leaf Litter
The shotgun metagenomics workflow begins with metagenomic DNA extraction followed by quality checks via Nanodrop, Qubit, and gel electrophoresis. Library preparation involves Enzymatic DNA fragmentation, adapter ligation, size selection using AMPure XP beads, and PCR amplification with Index barcoded primers, followed by quality assessment via Qubit and Agilent Tapestation. The prepared libraries are sequenced on an Illumina Nextseq 2000 with 2 x 150 PE sequencing chemistry with appropriate loading concentrations and sequencing run conditions.
SUB15156296
L001