GSM8798069: Blood, AD, ZWQ; Homo sapiens; RNA-Seq
Human basophils from patients with atopic dermatitis and healthy controls were isolated from 10 mL of peripheral blood using the EasySep™ Direct Human Basophil Isolation Kit for downstream scRNA-seq. A single-cell 3′ library was constructed using the 10x Genomics Chromium Single-Cell 3′ kit (v3). R1 (read 1 primer sequence) are added to the molecules during GEM incubation. P5, P7, a sample index, and R2 (read 2 primer sequence) are added during library construction via End Repair, A- tailing, Adaptor Ligation, and PCR. The final libraries contain the P5 and P7 primers used in Illumina bridge amplification.
GEO
GSM8798069_r1