GSM8554031: FD-1423-2-START; Homo sapiens; RNA-Seq
Total RNA was extracted from fixed whole blood using Qiagen PAXgene Blood RNA Kit following the manufacturer's instructions (Qiagen, Hilden, Germany). RNA quality control was performed using Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA) and RNA integrity was checked with 4200 TapeStation (Agilent Technologies, Palo Alto, CA, USA). RNA sequencing libraries were constructed using the NEBNext Ultra II RNA Library Preparation Kit for Illumina.Enriched RNAs were fragmented for 15 minutes at 94 °C. First-strand and second-strand cDNA were subsequently synthesized and fragments were end-repaired and adenylated at 3'ends.  Universal adapters were ligated to cDNA fragments, followed by index addition and library enrichment with limited cycle PCR.
GEO
GSM8554031_r1