TRNAU1AP knockout
Knockout of TRNAU1AP. Cell populations of lentivirally-transduced cells expressing Cas9 and gene-specific sgRNAs targeting TRNAU1AP (pAVA3129) that exhibited increased fluorescence were FACS-isolated. 2-3 ug of total cellular RNA was extracted from FACS-isolated cells and submitted to BGI-America, San Jose, CA for cDNA library construction (without the use of poly(A) selection) and DNBSEQ paired-end sequencing (100-bp paired-end reads). Ribosomal RNA was removed using RNase H-based kit and cDNA was synthesized using random hexamer primers and reverse transcription, according to BGI protocol.
SUB15720918
MMDM08