16SrRNAampliconseq_mouse
Genomic DNA was extracted from mouse faecal samples using the QIAGEN QIAmp Fast DNA Stool Mini Kit, amplification of the V3-V4 region of the bacterial 16S rRNA gene was amplified using the primers S-DBact-0341-b-S-17 and S-D-Bact-0785-a-A-21 together with Nextera DNA library prep kit adapters. Amplicons were sequenced using the Illumina NextSeq 1000 PE 2x300bp.
SUB14725522
GW111