16S rRNA amplicon sequencing of Enallodiplosis discordis: larval microbiome
DNA was extracted from pooled, surface-sterilized larvae of Enallodiplosis discordis using the ZymoBIOMICS DNA Miniprep Kit (Zymo Research). The 16S rRNA gene was amplified and barcoded with the Oxford Nanopore 16S Barcoding Kit 124. Sequencing libraries were loaded onto a MinION Mk1C with R9.4.1 flow cells, generating long-read 16S rRNA data.
SUB15580797
ED-02.2