UCE target enrichment of Leptanilla belantanoides, worker (CASENT0842867)
DNA was extracted non-destructively using a DNeasy Blood & Tissue Kit (Qiagen Inc., Valencia, CA) with DNA eluted from spin columns using buffer AE at 56 degrees Celsius (Cruaud et al. 2019). Genomic concentrations were quantified for each sample with a Qubit 2.0 fluorometer (Life Technologies Inc., Carlsbad, CA). Phylogenomic data were generated using the UCE probe set hym-v2 (Branstetter et al. 2017), with libraries being prepared and target loci enriched using protocols similar to Branstetter et al. (2017) at the Huntsman Cancer Institute, University of Utah (Salt Lake City, UT). Raw reads were assembled with SPAdes v. 3.12.0 (Bankevich et al. 2013). For trimming raw reads, use i7:GATCGGAAGAGCACACGTCTGAACTCCAGTCAC*ATCTCGTATGCCGTCTTCTGCTTG and i5:AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT*GTGTAGATCTCGGTGGTCGCCGTATCATT, where * indicates the index sequence.
SUB14804572
D3347