GSM9113454: LysoPS_OVA_1ug; Mus musculus; RNA-Seq
Each group consisted of six animals and collected mesenteric lymph node (MLN) at the end of the study. We obtained MLN cells suspension using enzymatic digestion and cells were removed using dead cell removal Samples with >70% cell viability were confirmed for single-cell RNA sequencing. The assays were conducted at the University at Buffalo Genomics and Bioinformatics Core. According to the Chromium Next GEM Single Cell 3' Reagent Kit v.3.1 (Dual Index) manual, samples were prepared and loaded with 10,000 cells per sample onto 10× Chromium chips (10× Genomics, Pleasanton CA). The 10× Chromium libraries were prepared and sequenced with dual barcoding, paired-end reads (28x,10i,10i,90x) on the NovaSeq 6000 platform using an SP flowcell (Illumina, San Diego, CA).
GEO
GSM9113454_r1