GSM9473850: Primary stromal cells, undifferentiated, donor OF01; Homo sapiens; RNA-Seq
For 10x scRNA-seq, cells have been trypsinized and spun down at 200 g and washed twice in culture media. Cells were separated using a 40 µm mesh, washed 3 times in PBS with 0.04% non-acetylated BSA, and cell numbers were adjusted to 0.7 million cells per ml followed by a final transfer through a 40 µm mesh. Bulk RNA was extracted from cultured cells using Trizol combined with Econo Spin columns (Epoch Life Sciences). scRNA-seq: Libraries were prepared according to manufactures instructions (10x Genomics). Bulk RNA-seq was performed according to manufacturer's instructions (TruSeq 2, Illumina) using 1 µg RNA for preparation of cDNA libraries.
GEO
GSM9473850_r1