GSM9001426: Neurons_TSA_19h_1; Rattus norvegicus; RNA-Seq
total RNA was extracted using ExtractRNA reagent (Evrogen, Russia) following manufacturer's protocol. mRNA was purified from a total RNA mixture using oligo(dT)-coated magnetic beads, followed by mRNA fragmentation and cDNA synthesis. The synthesized cDNA was then subjected to end repair, 3'-adenylation and adapter ligation. Following amplification of cDNA fragments, the PCR products were purified using Ampure XP Beads (AGENCOURT) and dissolved in EB solution. Library was validated on the Agilent Technologies 2100 bioanalyzer. The double-stranded PCR products were then heat denaturated and circularized using splint oligo sequence to generate a final library of single-stranded circular DNA molecules. The library was amplified using phi29 DNA polymerase to create a DNA nanoball containing more than 300 copies of a single DNA molecule.
GEO
GSM9001426_r1