RNA-seq of Saccharum hybrid POJ2878: mature leaf
This study was designed to obtain the transcriptomic profiles of mature leaves in Saccharum hybrid POJ2878. Total RNA was extracted from mature leaves collected from  a sugar cane grown at Shenzhen, China. RNA integrity was assessed using an Agilent Bioanalyzer, and samples with an RNA Integrity Number (RIN) > 8.0 were selected for library preparation. mRNA was enriched from total RNA using oligo(dT) magnetic beads. The purified mRNA was then fragmented and used as a template for first-strand cDNA synthesis with random hexamer primers, followed by second-strand synthesis. The resulting double-stranded cDNA was subjected to end-repair, A-tailing, and ligation with Illumina sequencing adapters. After PCR amplification, the final libraries were quantified and sequenced on an Illumina NovaSeq 6000 platform, generating 150 bp paired-end reads.
SUB15530546
POJ2878_RNA_seq_mleaf