madID-gDNA
genomic DNA was extracted using the Qiagen DNeasy Blood & Tissue Kit. gDNA was purified by treatment with RNase A (0.2 mg/mL final concentration) for 2h at 37C, followed by a 2 h incubation at 55C with proteinase K (0.2 mg/mL final concentration). The obtained gDNA was further purified by phenol/chloroform extraction and ethanol precipitation. Libraries were prepared using the native barcoding SQK-NBD114.96 kit using the recommendations for long gDNA fragments. Sequencing was performed using a MinION flow cell (R10.4.1) on a MinION sequencer. Reads were basecalled using dorado basecaller (v0.8.2) with options--emit-moves --emit-sam --reference PlasmoDB-64_Pfalciparum3D7_Genome.fasta sup@v5.0.0'
SUB14919912
madID-gDNA