16S rRNA Gene Sequencing of mus musculus: fecal samples
After the PE reads obtained by sequencing were split, the double-ended Reads were quality-controlled and filtered according to the sequencing quality. Meanwhile, the double-ended Reads were spliced according to the overlap between them to obtain optimized data after quality control splicing. The optimized data is then processed using Sequence denoising methods (DADA2/Deblur, etc.) to obtain Amplicon Sequence Variant (ASV) representing sequence and abundance information.
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OC_010