GSM8833713: WT B16-F10 lung metastatic model 3; Mus musculus; RNA-Seq
Lungs were digested to create single cell suspension. Then the rate of live cells were calculated. Novogene then complete the following extraction steps. Single-cell RNA sequencing (scRNA-seq) libraries were constructed using the 10x Chromium Single Cell 5' platform (10X Genomics, Pleasanton, CA, USA) according to the manufacturer's protocol. Briefly, single-cell suspensions were loaded onto a Chro-mium microfluidic chip to generate single-cell Gel Beads in Emulsion (GEMs). The scRNA-seq libraries were then prepared using the Chromium Single Cell 5' Reagent Kit v2(10X Genomics, Pleasanton, CA, USA). Library quality was assessed using the Ag-ilent 2100 Bioanalyzer system (Agilent Technologies, Santa Clara, CA, USA), and se-quencing was performed on the Illumina NovaSeq X Plus platform (Illumina, San Di-ego, CA, USA) with a sequencing depth of at least 50,000 reads per cell.
GEO
GSM8833713_r1