UCE target enrichment of Leptogenys_mayotte_MAMI0567_CASENT0132571
Samples were extracted using column-based extraction kits. DNA was sheared to 400-600 bp using a DNA sonicator. Genomic libraries were prepared using Kapa Hyper Prep Kits (1/4 volume reactions) and iTru, 8bp dual-indexing adapters (Glenn et al. 2019). Samples were pooled into pools of about 10 samples and enriched for ultraconserved elements (UCEs) using an ant-customized RNA bait set (Arbor Biosciences; ant-specific hym-v2) that includes 9,898 baits (120 mer) targeting 2,524 UCE loci shared across Hymenoptera and a set of legacy markers (Branstetter et al. 2017). Enriched pools were pooled together and sent to either the University of North Carolina at Chapel Hill genomics core facility or Novogene Inc. (Sacramento, CA) for sequencing on Illumina NextSeq or HiSeq instruments (2x150 bp).
SUB15092696
Leptogenys_mayotte_MAMI0567_CASENT0132571