Metagenomic Illumina Sequencing of Rattus norvegicus Rectal Swab Samples
RNA was extracted from rectal swab samples stored in TRIzol Reagent using the manufacturers protocol. Extracted RNA was treated with DNase, depleted of ribosomal RNA, and reverse transcribed to double-stranded cDNA using the RAPIDprep assay protocol. Sequencing libraries were prepared from purified cDNA using the NEBNext Ultra II DNA Library Prep Kit for Illumina with NEBNext dual index primers. Bead-based cleanup steps used Mag-Bind Total Pure NGS beads. Library quality was assessed using the Agilent 4150 TapeStation and Qubit quantification. Libraries were pooled and sequenced on an Illumina NextSeq 2000 using the 300-cycle P2 v3 cartridge with 2  150 bp paired-end reads.
SUB15421791
PoolMMAR0480-83RNA