PacBio-Revio of Plestiodon fasciatus: juvenile female blood
Genomic DNA was extracted from blood stored in EDTA with the Qiagen MagAttract High Molecular Weight DNA kit following their Manual Purification of High-Molecular Weight Genomic DNA from Whole Blood protocol from the MagAttract HMW DNA Handbook in the AMNH ICG. The extracted sample was then sent to Azenta/Genewiz, where it was sequenced with PacBio Revio HiFi sequencing on 1 SMRT cell, which typically results in ~15 million reads and ~100GB of data, depending on the quality of the input sample. We expected a coverage of ~66x based on an estimated 1.5GB genome size. The sequencing generated ~6.3 million read pairs and ~79GB of data, with an average read length of ~12,000bp, resulting in ~53x coverage.
SUB15432005
Pfas_AMNH-FS-20603_longread