GSM9324564: DKOCr298_H3K27ac_1, ChIP-seq; Mus musculus; ChIP-Seq
ChIP experiments were performed as previously described (doi:10.1038/nprot.2006.27) using anti-H3K27Ac (Active Motif 39133) or rabbit IgG (Millipore 12-370). DNA quantitation was performed using the Qubit HS dsDNA. Assessment of fragment size was done with the TapeStation HS D1000 assay. Sequencing libraries were prepared using the Swift Accel-NGS 2S Plus DNA kit and 2S Combinatorial Dual Indexing kit, according to the manufacturer's instructions. Each library was prepared with 10 ng of DNA. The final library amplification was performed with 6 cycles of PCR amplification. Quality control of the libraries was done with the Qubit HS dsDNA and TapeStation HS D1000 kit. Libraries were quantitated using the KAPA Library Quantitation Kit for Illumina platforms. The concentrations obtained were used to pool the libraries, and this pool was submitted to the UM Advanced Genomics Core for sequencing on the NovaSeq6000, using an S1 flow cell and paired-end 50 nucleotides chemistry.
GEO
GSM9324564_r1