GSM9115022: CUT&Tag input sample from rat pancreas with acute pancreatitis; Rattus norvegicus; OTHER
Pancreatic tissues were rapidly harvested and flash-frozen. Samples were then pulverized, and nuclei were extracted using standard CUT&Tag nuclear extraction buffers to ensure nuclear integrity for subsequent assays. CUT&Tag libraries were prepared using a transposase-mediated method. A transposase mix containing transposase and two equimolar adapters (Adapter 1 and Adapter 2) was added to the sample and incubated at 37°C for 30 minutes. This process simultaneously fragments DNA and attaches sequencing adapters. Libraries were then PCR-amplified, size-selected, and purified to generate the final sequencing-ready libraries.
GEO
GSM9115022_r1