GSM8728958: KR1 biol rep 1; Homo sapiens; RNA-Seq
Cells were harvested at 5 dpi, centrifuged at 1,000 rpm for 10 minutes to remove the supernatant, and subjected to at least one freeze-thaw cycle using a -80°C freezer. Nucleic acid extraction was then performed with the Quick-DNA/RNA Miniprep Plus Kit (Zymo Research, USA), following the manufacturer's protocol. Genomic DNA was removed with DNase I (Zymo Research, USA). After DNA degradation, RNA samples were assessed for purity and prepared using the Illumina Stranded mRNA Prep, Ligation Kit (Illumina, USA) according to the manufacturer's instructions. Sequencing was conducted on the Illumina NovaSeq 6000 system (Illumina, USA) with paired-end 150 bp reads (PE150). Libraries were normalised to 0.5 nM, pooled, and loaded onto the NovaSeq platform.
GEO
GSM8728958_r1