Iso-Seq data of Oryza rufipogon
Total RNA was extracted from leaf tissue and developing seeds collected at five developmental stages (5, 10, 15, 20, and 25 days post-anthesis) from Oryza rufipogon. Equal amounts of RNA from all samples were pooled to construct full-length cDNA libraries using the PacBio Iso-Seq protocol. cDNA synthesis was performed using the SMARTer PCR cDNA Synthesis Kit, followed by amplification with a high-fidelity polymerase. Size selection was performed to generate both nonsize-selected and >4 kb libraries, which were subsequently converted into SMRTbell templates and sequenced on the PacBio Sequel platform. Raw sequencing data were processed using the IsoSeq3 pipeline in SMRT Link, including generation of circular consensus sequences (CCS) and identification of full-length non-chimeric (FLNC) reads.
SUB16109877
Ruf_Iso-Seq