SH_MS_2 (CRX1781823)
RNA was extracted using CTAB-pBIOZOL, and mRNA with a polyA tail was enriched using Oligo(dT) magnetic beads; the purified mRNA was fragmented into small pieces; first-strand cDNA was synthesized through reverse transcription using random hexamer primers; second-strand cDNA synthesis was performed, along with end-repair and dA-tailing; sequencing adapter ligation was carried out, followed by DNA magnetic bead purification and fragment selection to obtain a library with insert fragments of 250-350 bp.
NGDC
CRX1781823
South China Agricultural University
DRX1000385