GS
Total papaya flesh RNA was extracted with RNAprep Pure Plant Plus Kit (Tiangen). RNA concentration and purity was conducted using NanoDrop 2000 (Thermo Fisher Scientifi), and RNA integrity was assessed using the RNA Nano 6000 Assay Kit (Agilent Technologies). For RNA sequencing, 1.0 g of RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB) following manufacturers recommendations and index codes were added to attribute sequences to each sample. Index codes were used to label sequences from each sample for mRNA sequencing.
SUB14939351
R24113302