GSM8553438: EZIMR90_48hrs_Samples1-6; Homo sapiens; RNA-Seq
Total RNA was isolated from plated down EZ sphere cells that were maintained in the serum-supplemented medium (aged, young, or serum-free) for 48 hours using Direct-zol RNA Kit (Zymo Research, Irvine, CA, USA) Libraries were prepared by BRB-Seq (Alpern D, et al. 2019) method. Individual samples were reverse transcribed using dT primer with unique cell barcodes. Then replicate were mixed and amplified by PCR. 10nt of UMI followedby 9nt of cell barcode are in read1 and 81nt of RNA insert reads are in read2.
GEO
GSM8553438_r1