GSM9114824: Evs of  CONTROL-GESTorg pool 2; Homo sapiens; miRNA-Seq
miRNA was isolated from EVs secreted by ENDO-GESTorg and CONTROL-GESTorg using the miRNeasy Serum/Plasma Kit (Qiagen, Germantown, MD, USA) according to manufacturer's instructions. miRNA NGS libraries were generated using the QIAseq miRNA Library Kit (Qiagen, Germantown, MD, USA). Briefly, after adapter ligation, UMIs were introduced in the reverse transcription step, and cDNA was amplified using PCR (22 cycles) and subsequently, samples were purified. Library preparation was quality controlled using capillary electrophoresis (Tape D1000). Based on the quality of the inserts and the concentration measurements the libraries were pooled in equimolar ratios (n= 4 samples/pool of ENDO-GESTorg and n= 3 samples/pool of CONTROL-GESTorg).The library was sequenced on a NextSeq 2000 sequencing instrument (Illumina Inc, San Diego, CA, USA) according to the manufacturer instructions (1x75, 1x6). Raw data was de-multiplexed and FASTQ files for each sample were generated using the bcl2fastq2 software v2.20.0.422 (Illumina inc.).
GEO
GSM9114824_r1