GSM9216894: Mx1-T3-2; Mus musculus; ATAC-seq
HSCs were sorted using a BD Aria cell sorter. A total of 50,000 HSCs were resuspended in ice-cold cryopreservation solution (50% FBS, 40% growth media, 10% DMSO). Growth media contained StemSpan SFEM (StemCell Technologies) supplemented with 10% ES-Cult FBS (StemCell Technologies), 1% penicillin/streptomycin (Invitrogen), IL-3 (20 ng/ml), IL-6 (20 ng/ml), TPO (50 ng/ml), Flt3L (50 ng/ml), and SCF (100 ng/ml) (Peprotech). HSCs were frozen in a pre-chilled Mr. Frosty container, placed at −80 °C overnight to complete cryopreservation, and then submitted to Active Motif (Carlsbad, CA, USA) for ATAC-seq. ATAC-seq libraries were prepared by Active Motif following their standard workflow and sequenced on an Illumina NovaSeq 6000 platform, generating paired-end 42 bp reads (2 × 42 bp).
GEO
GSM9216894_r1