GSM9114521: Inguinal WAT, Cebpa_fl/fl 1; Mus musculus; RNA-Seq
Both inguinal white adipose tissue depots were dissected out and extracted together and lymph nodes were removed from each mouse. RNA was extracted with the Qiagen Lipid Tissue Kit. The Columbia University Medical Center Sequencing Core generated the libraries after submission of RNA samples. First, poly-A pull-down was used to enrich mRNAs from total RNA samples. Then, library construction was done using Illumina TruSeq chemistry. The final PCR step has been modified using the KAPA HiFi HotStart Ready Mix.
GEO
GSM9114521_r1