GSM8972168: BxPC3s, 28clones, scRNA; Homo sapiens; RNA-Seq
Cultured cells were detached using trypsin and passed through a cell strainer to obtain a single-cell suspension. The resulting suspension was cryopreserved in Bambanker (Nippon Genetics) for later use in downstream experiments. Cryopreserved cell stocks were rapidly thawed at 37 °C, and cell viability was confirmed to exceed 90% before further processing. Equal numbers of viable cells were pooled and loaded onto a 10x Genomics Chromium Controller for single-cell partitioning and barcoding. cDNA synthesis and library preparation were performed using the Chromium Single Cell 3′ v3 protocol (10x Genomics) according to the manufacturer's instructions.
GEO
GSM8972168_r1