Replicate 4 10mM-B
Total RNA was extracted from transfected HEK293T cells using TRIzol reagent (Invitrogen) following the manufacturers protocol. The purity and integrity of the isolated RNA were assessed using a Qubit 2.0 fluorometer (Life Technologies, USA) and an Agilent 2100 Bioanalyzer (Agilent Technologies, USA). These samples were DNase treated, mRNAs were enriched, and sequencing libraries were constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, USA). Libraries were sequenced on an Illumina NovaSeq X Plus using 25B Flowcell and 2x150bp configuration (Illumina, San Diego, CA, USA). Sequence coverage and quality metrics were calculated and preliminary RNA-seq data analysis was performed by Azenta Life Sciences (Genewiz, USA).
SUB15745802
T4-H-RNAi-10nM-B