GSM9145096: BAL Female Organoid; Homo sapiens; RNA-Seq
Organoids were dissociated into single cells by incubation in TrypLE Express Enzyme at 37°C for 1-2 hours with gentle pipetting performed every 5-10 minutes. Cell viability was assessed using Trypan Blue exclusion. Cells were passed through a 40-μm nylon mesh filter to remove large clumps and debris, then centrifuged at 200g for 10 minutes. The cell pellet was resuspended in DMEM + 10% FBS to achieve a final concentration of 1000-1200 cells/μL for single-cell library preparation. Single-cell RNA sequencing libraries were generated using 10x Genomics Chromium Next GEM Single Cell 3' v3.1 kits for dual-index, paired-end sequencing. Library preparation was performed at the CHOP Single Cell Technology Core following manufacturer's protocols. Quality control of libraries was performed using Qubit for quantification and Bioanalyzer for size distribution analysis.
GEO
GSM9145096_r1