GSM8766850: Dock2flox_3; Mus musculus; OTHER
Total genomic DNA was extracted using the OMEGA Soil DNA Kit (M5635-02). For fecal 16S rDNA sequencing, bacterial genomic DNA (>500 ng) was amplified targeting the V3-V4 region with primers (forward: ACTCCTACGGGAGGCAGCA; reverse: GGACTACHVGGGTWTCTAAT) containing 7-bp barcodes. PCR products were purified (Vazyme VAHTSTM DNA Clean Beads) and quantified (Quart-IT PicoGreen dsDNA Assay Kit). Amplicons were pooled in equal amounts, and pair-end 2250 bp sequencing was performed on the Illumina NovaSeq platform (NovaSeq 6000 SP Reagent Kit).
GEO
GSM8766850_r1