low coverage whole genome resequencing (WGS) of Iberian Squalius species
Total genomic DNA was extracted from ethanol preserved fin clips. Genomic DNA was randomly sheared, the resulting fragments were end-repaired and A-tailed, followed by ligation with the Illumina adapters. Fragments with adapters were size-selected, PCR amplified and purified. The resulting libraries were paired-end sequenced at 150bp on Illumina NovaSeq6000. For some individuals (biosamples), to obtain the desired amount of data the library was sequenced multiple times, hence the multiple pairs of files. All library preparation and sequencing was performed at Novogene, UK.
SUB15044553
AT30167_EKDN230012711-1A