GSM8729692: E13.5M PGCs IUHG  bio rep1; Mus musculus; ATAC-seq
The EGFP-positive PGCs obtained through FACS were washed with PBS. 5,000-10,000 cells were collected and lysed using lysis buffer. ATAC-seq was performed using the Hyperactive ATAC-Seq Library Prep Kit (Vazyme).  The DNA was extracted as starting material for samples. Libraries was amplified using 15 cycles on the thermocycler and were validated using the Equalbit 1 × dsDNA HS Assay Kit (Vazyme)  for sequencing.
GEO
GSM8729692_r1