Amplicon sequencing of metagenome: diversity and distribution in Fritillaria thunbergii bulb and rhizosphere soil
The sequencing library targets the bacterial 16S rRNA V5V7 region and the fungal ITS region of the Fritillaria thunbergii-associated metagenome. Rhizosphere soil components were separated from roots via vortexing with PBS buffer and 80-mesh sieving. Bulb samples underwent a stringent surface sterilization using 70% ethanol and 2.5% sodium hypochlorite prior to DNA extraction. The 16S V5V7 region was amplified using primers 799F and 1193R (13 cycles), and the ITS region was amplified using ITS1F and ITS2R (27 cycles), with a Pro Taq premix and an annealing temperature of 55 C. Amplicon libraries were prepared using dual-index barcodes and sequenced on the Illumina MiSeq platform with a paired-end 252 bp strategy.
SUB15759321
BHG_3_ITS