Transcriptomic profiling of immature tomato fruits grown hydroponically under high salt, low-phosphate and their combination
Transcriptomic profiling of immature tomato fruits grown hydroponically under high salt, low-phosphate and their combination
Pericarp tissues were separated from fruits using a scalpel, and the samples were frozen in liquid nitrogen. Tomato seedlings were grown hydroponically with different concentrations of nutrients and salts. EC12, P10 and EC12 refers to a high salt condition (EC12, 94 mM NaCl), a low phosphate solution (10% of control) and the combination of both. Samples were ground in liquid nitrogen into powder. Total RNA was isolated from the powdered samples using RNA iso plus (TaKaRa). One sample is from one fruit. Library was constructed as described in Lasy-seq protocol (https://sites.google.com/view/lasy-seq/)
European Bioinformatics Institute
E-MTAB-16436:15DAP_EC12_2_s