GSM8728739: MiaPaCa-2 cells, siSLC25A1,  replicate 1; Homo sapiens; RNA-Seq
Total RNA was extracted using PureLink RNA isolation (Life Technologies, 12183025) and treated with DNase (Life Technologies, AM2222). mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The end repair, A-tailing, adapter ligation, size selection, amplification, and purification were performed.
GEO
GSM8728739_r1