RNA-Seq of sludge inoculum at low pressure
Total RNA was extracted from incubation samples using an RNA extraction kit (Majorbio, shanghai, China) according to the manufacturers protocols. RNA concentration was measured using a Qubit V.4.0 Fluorimeter (Thermo Fisher Scientific, USA) and the quality was evaluated using a bioanalyzer (JY600C, Beijing JUNYI Electrophoresis Co., Ltd., China). rRNA in samples were removed using Ribo-Zero rRNA Removal Kits (Lexogen, USA) as per the manufacturers instructions. Enriched mRNAs were used to construct an mRNA-enriched metatranscriptomic library using a TruSeq RNA Sample Prep Kit (Illumina, USA). The metatranscriptomic library was sequenced using an Illumina HiSeq 4000 (paired-end; insert size, 300 bp; read length, 150 bp).
SUB15141860
RNA-LP-s