lignite enrichment culture_V3V4
DNA was extracted from enrichment culture using PowerSoil PRO DNA Extraction kit (Qiagen). Isolated DNA was subjected to amplification of V3V4 hypervariable region of 16S rRNA gene using 341F and 785R primers. Library preparation was conducted using standard Illuma protocol. Sequecing was performed on Illumina MiSeq platform with 2x250 paired ends approach.
SUB14889088
lignite_enrichment_4