V3-V4 regions of the 16S rRNA gene of Bacteria: Mus musculus feces
Total DNA was isolated from each fecal sample (200 mg per sample) using a QIAamp DNA stool mini kit following the manufacturers protocol. The V3-V4 regions of the 16S rRNA gene were amplified using universal region-specific primers 341F and 806R connecting with sample-identifying barcodes. All PCR reactions were carried out with Phusion High-Fidelity PCR Master Mix. The DNA libraries was generated basesd on the Illumina Novaseq 6000 platform, and quantified via Qubit and Q-PCR.
SUB15747071
CBDI56.3