GSM9324754: TBP; Mus musculus; RNA-Seq
Thyroid tissues were minced into pieces and digested in HBSS containing 1 mg/mL Collagenase Type I (Sigma) and 1 mg/mL Collagenase Type II (Worthington) at 37℃ for 1 h. Live cells were sorted by FACS. Single cell gel beads in emulsion (GEMs) generation, barcoding, post GEM-RT cleanup, cDNA amplification, and cDNA library construction were performed using Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (10x Genomics), according to the manufacturer's protocol. The libraries were sequenced on Illumina Nova 6000 PE150.
GEO
GSM9324754_r1