GSM8729033: Library B-LN, Experiment 2, Gene expression; Mus musculus; RNA-Seq
Cell suspensions from each biological sample were labeled with BioLegend hashtaging antibodies Total-seq-C before pooling and sorting on tdTomato+ cells. In each experiment a sample from each condition was used in the pool, with a total of 3 pooled libraries for experiment 1, and 2 pooled libraries for both brain and lymph node for experiment 2. Libraries were loaded on a 10X Genomics Chromium Single Cell Controller with volumes aiming at a targeted recovery of 20'000 cells per channel, and libraries were prepared using the 5'end v2 kit. Sequencing was performed on an Illumina NovaSeq 6000 sequencer at the Genomics Facility Basel of the ETH Zurich
GEO
GSM8729033_r1