RNA-Seq of Prorocentrum cordatum: cells subjected to cooling for 7 h, biological replicate 1
Standard cultivation conditions: 12 h light:12 h dark cycle at 100 mol photons m2 s1 and 18 C. Samples of this type were placed in a refrigerator equipped with a lamp for algae cultivation and were additionally overlaid with ice. The temperature of the samples was 2 . The samples were incubated in the refrigerator for 7 hours.Total RNA from P. cordatum cells was extracted using HiPure Plant RNA Kit (R415102 Magen, China; the lysis protocol was modified). The sequencing libraries were prepared with TruSeq Stranded mRNA kit (Illumina, USA) following the manufacturers recommendations. The quality of the libraries was assessed using the Bioanalyzer system.
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