="UCE target enrichment of "&B5&"."
Samples were extracted using Zymo Quick DNA kits. DNA was sheared to 400-600 bp using a QSoncia Q800R sonicator. Genomic libraries were prepared using Kapa Hyper Prep Kits (1/4 volume reactions) and iTru, 8bp dual-indexing adapters (Glenn et al. 2019). Samples were pooled into pools of approximately 10 samples and enriched for ultraconserved elements (UCEs) using an ant-bee customized RNA bait set (Arbor Biosciences; myBaits UCE Hymenoptera 2.5Kv2AB) that targets 2,545 UCE loci shared across Hymenoptera and a set of legacy markers (Branstetter et al. 2017; Grab et al. 2019). Enriched pools were pooled together (up to 110 individual samples) and shipped to either the University of Utah genomics core facility for sequencing on an Illumina HiSeq 2500 instrument (2x125 bp v4 chemistry) or to Novogene in Sacramento, CA for sequencing on HiSeqX.
SUB15958676
Dasypoda_rosaella_BLX3259