GcKO_PAGC2
The amount of RNA from 80 oocytes was used for the construction of sequencing libraries.RNA libraries for RNA-seg were prepared using SMARTER mRNA-Seg Library Prep Kit following manufacturers protocols.Data quality control.Use FastQC data quality inspection tool in the analysis to check for low-quality bases and contaminated sequences.The original reads were filtered using the Trimomatic (v0.39) tool to remove adapter motifs, low-quality bases, and contaminated sequences.Align the filtered reads with the reference genome using STAR (version 2.7.9a) (parameters: runThreadN 30 alignintroMax 100000 chamSegmentReadGapMax parameter 3 alignSJstitchMismatchNmax 5-155).After comparing with the reference genome, the expression level was calculated using features (v1.6.5), and standardized gene level FPKM and TPM values were obtained using $ring Tie (v2.1.7),Use DEseq2 to analyze the differences in gene count matrices with duplicate samples within the group. Use edge analysis to analyze the differences between groups without duplicate samples.
SUB14828611
GcKO_PAGC2