GSM9440539: proto_in_vitro_symbiotic, replicate 1; Dactylorhiza majalis; RNA-Seq
The samples were homogenized in liquid nitrogen using TissueLyser II (Qiagen) in 2 mL Eppendorf tubes containing ceramic beads. Homogenized material was subjected to the NucleoZol (Macherey-Nagel, Dueren, Germany) reagent extraction process following the manufacturer's protocol. RNAs were further purified using Agencourt RNAClean XP (Beckman Coulter, Brea, CA, United States) magnetic beads following the manufacturer's instructions. The libraries were constructed using the TruSeq Stranded mRNA kit (Illumina, San Diego, CA, United States).
GEO
GSM9440539_r1