GSM9083225: NA73-Day25-ARTposVenetoclaxposantiposCD8; Macaca mulatta; RNA-Seq
CD4+ T-cells were lyse in 350 L of Buffer RLT and then extracted using the RNeasy Mini / kit (Qiagen) with on-column DNase digestion. RNA quality was assessed using a TapeStation 4200 (Agilent) Ten nanograms of total RNA was used as input for cDNA synthesis using the SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio) according to the manufacturer's instructions. Amplified cDNA was fragmented and appended with dual-indexed barcodes using the Nextera XT DNA Library Preparation kit (Illumina). Libraries were validated by capillary electrophoresis on a TapeStation 4200 (Agilent), pooled at equimolar concentrations, and sequenced with PE100 reads on an Illumina NovaSeq 6000, yielding ~21 million reads per sample on average.
GEO
GSM9083225_r1