16S_B_11
Leech bloodmeal-derived iDNA was analyzed by mitochondrial metabarcoding using vertebrate-specific mt12S and mammalian-specific mt16S primers with Illumina adaptor-compatible sequences. Amplicons were generated by PCR, purified, quality-checked by Qubit and Fragment Analyzer, indexed using Illumina UD indexes, pooled, and sequenced on an Illumina NextSeq 2000/MiSeq platform with paired-end 2  150 bp reads.
SUB16280535
LGP24-WJ174