GSM8972470: Roots_ML4xWD_1; Citrus sinensis; RNA-Seq
Total RNA was extracted from 100 mg of leaves and roots frozen powder using the RNeasy® Plant Mini kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. RNA quality and quantity were determine using a mySPEC microvolume spectrophotometer (VWR, Radnor, PA, USA). High purity RNA samples (OD260/OD280 values at 1.8-2.0) were used for library construction and sequencing. For each RNA sample, cDNA libraries were prepared for sequencing from 1 µg of total RNA using the Truseq Stranded mRNA kit (Illumina, San Diego, CA, USA) following the manufacturer's protocol.
GEO
GSM8972470_r1