Cane toad whole genome sequencing - 10x linked-read sequencing 1
High-molecular weight genomic DNA (gDNA) was extracted from cane toad liver tissue using the genomic-tip 100/G kit (Qiagen, Hilden, Germany). This was performed with supplemental RNase (Astral Scientific, Taren Point, Australia) and proteinase K (NEB, Ipswich, MA, USA) treatment, as per the manufacturer's instructions. Isolated genomic DNA was further purified using AMPure XP beads (Beckman Coulter, Brea, CA, USA) to eliminate sequencing inhibitors. DNA quantity was assessed using the Quanti-iT PicoGreen dsDNA kit (Thermo Fisher Scientific, Waltham, MA, USA), DNA purity using a Nanodrop spectrophotometer (Thermo Fisher Scientific), and integrity using pulse-field gel electrophoresis. A 10x Genomics (Pleasanton, USA) linked-read library was prepared using the Chromium™ Genome Reagent Kit (v2 Chemistry) and sequenced (2 × 150 bp paired-end) on the Illumina HiSeq X Ten platform.
UNIVERSITY OF NEW SOUTH WALES
TOAD_10X_1