scRNAseq of WT Brachypodium distachyon: sample 2022, first sequencing run
Tissue was digested with macerozyme R10 and Cellulase Onozuka RS and subsequently washed to obtain protoplasts. GEM wells were prepared to encapsule protoplasts in droplets, cDNA was extracted per protoplast and libraries were prepared according to the guidelines provided by 10X Genomics (Chromium Next GEM single cell 3' version 3)
SUB15576896
WT2022-1