Metabarcoding approach for the characterisation of soil biodiversity in soils from dfferently managed rice agroecosystems
eDNA extraction using the NucleoSpin Soil, PCR-based library preparation using 18S primer pair TAReuk454FWD1/ TAReukREV3 (Stoeck et al., 2010) with Illumina overhangs for indicisation. The KAPA HiFi HotStart ReadyMix kit (Roche Molecular Systems) was used for the library construction.
SUB15922667
R1.3A_18S