GSM9178744: CT_Ly6c_H2; Mus musculus; RNA-Seq
RNA from purified MC subsets were extracted from individual mice and pooled (5 mice each group), and subjected for bulk RNA sequencing in duplication on NextSeq 500 for CT mice and Illumina Hiseq 4000 sequencer for diabetic mice. The raw RNA-seq data contains 40 million reads in each sample and analyzed by using R Programing language. Raw sequencing reads in FASTQ format were processed using Kallisto (v0.45) for pseudoalignment and transcript quantification. Gene-level expression values were obtained using tximport (1.12.3) in R (3.6.1) to summarize transcript-level estimates to gene counts and transcript per kilobase per million mapped reads (TPM) values for downstream analysis. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO
GSM9178744_r1