GSM9322661: Baf3_SAFB1+2_KO_sgRNA_2_1; Mus musculus; miRNA-Seq
Total RNA was isolated using TRIZOL, and 400 ng of total RNA were spiked with a defined mix of the following reference small RNAs: spike-in 1 pNNNNACGATCGGCTCTANNNN; spike-in 2 pNNNNTGAACGTCCGTACNNNN; spike-in 3 pNNNNTCTCGCGCGCGTTNNNN; spike-in 4 pNNNNCGAGTAATGAACGNNNN. 100 ng of this total RNA + spike-in mix was then used for library preparation. Libraries were constructed with the QIAGEN QIAseq miRNA Library Kit according to the manufacturers recommendation.
GEO
GSM9322661_r1