GSM9083447: WT1year1; Mus musculus; RNA-Seq
Muscle tissue was removed, rapidly frozen on dry ice, and RNA was extracted using Trizol reagent. Messenger RNA was purified from total RNA using poly-T oligo-attached  magnetic beads. After fragmentation, the first strand cDNA was  synthesized using random hexamer primers followed by the second  strand cDNA synthesis. The library was ready after end repair, A-tailing,  adapter ligation, size selection, amplification, and purification. The  library was checked with Qubit and real-time PCR for quantification and  bioanalyzer for size distribution detection
GEO
GSM9083447_r1