Genome sample of Dermabacteraceae bacterium P13147
Whole-genome DNA extraction from all 17 investigated strains was performed using the High Pure PCR Template Preparation Kit (Roche) in accordance with the protocol provided by the manufacturer. Input DNA was quantified using the Quantus fluorometer with the Quantifluor dsDNA kit (Promega). A total of 200 ng of DNA was used for library preparation, which was carried out using the xGen DNA EZ Library Prep Kit (IDT) according to the manufacturers protocol. DNA fragmentation was performed for 16 minutes, followed by 6 cycles of PCR amplification. Purification steps were conducted using Ampure SPRIselect beads (Beckman-Coulter). For sample indexing during the final PCR step, xGen UDI primer sets were employed. The resulting libraries were quantified, pooled, and sequenced on the Illumina NovaSeq S6000 platform using a paired-end 2161 bp configuration. Libraries that failed to yield a sufficient number of reads were converted into MGI-compatible libraries via circularisation and subsequently sequenced on the MGI G400 instrument using a paired-end 2150 bp mode.
SUB16283540
P13147_1