GSM9114631: 68993_GF; Mus musculus; ATAC-seq
For ATAC-seq, freshly FACS-isolated tdTomato+ live Paneth cells from GF or SPF Paneth cell reporter mice were processed according to the OMNI-ATAC-Seq protocol Samples were then purified with the Zymo DNA Clean and Concentrator-5 kit and pre-amplified using the NEBnext 2X Master Mix for 5 cycles. 5 μL of the pre-amplified sample was subjected to qRT-PCR amplification for additional 20 cycles to determine the optimal additional cycles of the remaining 45 μL of sample. The PCR-amplified libraries were purified; fragment size was selected using Pippin Prep and sequenced on Illumina NextSeq 150 cycle mid output
GEO
GSM9114631_r1