GSM9691125: NRCM cells，H/R-AS8351, H3K4me3-2 (CUT&Tag); Rattus norvegicus; OTHER
1×10⁵ NRCMs per sample were harvested and subjected to CUT&Tag analysis using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (ABclonal, RK20265) according to the manufacturer's instructions. Primary antibodies against H3K4me3 (1:50, ABclonal, A2357) or Kdm5b (1:50, ABclonal, A7772) were added and incubated overnight at 4°C. After incubation with anti-Rabbit IgG secondary antibody, samples were treated with pAG-Tn5 transposase, followed by tagmentation and DNA purification. Libraries were amplified with indexed primers.
GEO
GSM9691125_r1