GSM8729356: p65 KO HEK293A cells, p65 2CS_7; Homo sapiens; RNA-Seq
Total RNA was isolated with RNeasy Mini Kit (74104, QIAGEN) and analyzed using Bioanalyzer (Agilent Technologies). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library. For the directional library, it was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, andpurification
GEO
GSM8729356_r1