DNA-seq of lateritic soil of Birbhum replicate 1
DNA was isolated from all the soil samples using DNeasy PowerSoil kit (100) Cat. No. 12888-100 (Qiagen) according to the manufacturers protocol. PCR amplification of V3 regions of all bacterial 16S rRNA genes was done from the metagenome of soil samples using Bacteria-specific universal oligonucleotides. For simultaneous sequencing of multiple PCR products on a single Ion chip, each DNA templates from an individual sample was PCR amplified using a 16S forward primer prefixed with an Ion Torrent adapter and a unique sample-specific barcode or multiplex identifier. The PCR-products were sequenced on Ion S5 (Thermo Fisher Scientific, USA) sequencer system.
SUB14677280
BIR1