GSM9769561: BPH982, BPH/2J, vehicle, LV; Mus musculus; RNA-Seq
Total RNA was extracted using the Aurum™ Total RNA Mini Kit (Bio-Rad Laboratories, Hercules, CA, USA). RNA concentration and purity were determined using NanoDrop One spectrophotometer UV–Vis (Bio-Rad Laboratories, USA). RNA samples (50 ng/µL) were reverse-transcribed into cDNA using the iScript cDNA Synthesis Kit on a T100™ Thermal Cycler (Bio-Rad, USA). Total RNA was used to construct libraries using the VAHTS® Universal V8 RNAseq Library Prep Kit for Illumina NR605 and sequenced using Illumina NovaSeq6000 S4 flowcell PE150 sequencer at Azenta Life Sciences (Hangzhou, China).
GEO
GSM9769561_r1