GSM9386707: stg5 lateCM CUTnRUN H3K27me3 rep1; Homo sapiens; OTHER
dcChIP-seq: 5 x106 cardiomyocytes or hiPSCs were first double crosslinked using 2 mM DSG for 35 minutes, followed by 1% formaldehyde for an additional 10 minutes. Nuclei were subsequently isolated, and the chromatin was lysed using 1% SDS buffer. The lysate was sonicated with a Covaris S220 (175 peak incident power, 10% duty factor, 200 cycles/burst) for 430 seconds to yield soluble chromatin. The resulting chromatin was incubated overnight at 4°C with primary antibodies (EZH1 (Proteintech, 20852-1-AP), EZH2 (CST, 5246), H3K27me3 (CST, 9733)) conjugated to Dynabeads Protein A. Following sequential washes with Buffers A, B, and C, the chromatin was reverse crosslinked overnight at 65°C after 37°C RNase treatment. The resulting DNA fragments of about 200 bp were purified using AMPure XP beads.  CUTnRUN: CUT&RUN was carried out following the Epicypher protocol. dcChIP-seq: Libraries were prepared with NEB DNA library prep kit (NEB, E7645L) following the manufacturer's protocol.  CUTnRUN: Libraries were prepared with NEB DNA library prep kit (NEB, E7645L) following the manufacturer's protocol.
GEO
GSM9386707_r1