16S rRNA microbiome sequencing from neonate blood (NICU
Total DNA was extracted from human blood. Then, 10 ng of DNA was barcoded and amplified using the SQK-16S114.24 16S Barcoding Kit, followed by PCR and nested PCR. A total of 60 fmol of the pooled PCR product was sequenced on a Flongle using the GridION platform. Analysis was performed using the EPI2ME 16S workflow.
SUB15428603
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