16S of LEO soil microbial community: S4CB_16S
DNA was extracted from approximately 250 mg of homogenized soil using the FastDNA Spin Kit for Soil (MP Biomedicals, USA) following the protocol. DNA quantity and quality were assessed using a Qubit dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific, USA). The V4 hypervariable region of the bacterial and archaeal 16S rRNA gene was amplified using the primer pair 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3'). Amplicon libraries were prepared following Illumina library preparation protocols with minor modifications and sequenced on an Illumina MiSeq platform using paired-end 2  150 bp chemistry.
SUB16050555
S4CB_16S_paired_illumina