GSM8971957: D35 days, Control, rep2; Homo sapiens; RNA-Seq
Cells were washed with PBS and scraped into 1000μl Trizol reagent. 200μl of chloroform was added, and the samples were incubated at room temperature for 5 minutes. The samples were centrifuged at 12,000 g, and the aqueous phase was collected. 500μl of isopropanol was added to precipitate RNA, and the sample was incubated for 25 minutes at room temperature, followed by centrifugation at 12,000 g for 10 minutes at 4°C. The RNA pellet was washed with 75% ethanol, semi-dried, and resuspended in 30μl of DEPC-treated water. After quantification and adjustment of the volume of all the samples to 1μg/μl, the samples were treated with DNAse (New England Biolabs #M0303). 10μl of this volume was used to generate cDNA using the manufacturer's protocol (Thermo Fisher #4368814). Novogene in-house protocol
GEO
GSM8971957_r1