GSM8798235: AAV8-HLP-mGHR (4E10 vg), Male: KC14-008; Mus musculus; RNA-Seq
Total RNA from the livers of treated mice was isolated using the AllPrep DNA/RNA Mini Kit (QIAGEN), according to the manufacturer's instructions. polyA-RNA was isolated from total RNA using oligo-dT magnetic beads. After fragmentation, first strand cDNA was synthesized using random hexamer primers and second strand cDNA was synthesized using dUTP to obtain a directional (stranded) sequencing library was obtained after end repair, A-tailing, adapter ligation, size selection, enzymatic digestion, amplification, and purification. Libraries were quantified with Qubit and real-time PCR and Bioanalyzer for size distribution detection. Quantified libraries were pooled and sequenced on an Illumina NovaSeq 6000 instrument at Novogene-AIT Genomics, Singapore.
GEO
GSM8798235_r1