16S rRNA amplicon sequencing of Leptosynapta tenuis, coelomic fluid sample, animal A
DNA was extracted from coelomic fluid that was collected via vacuum onto a 0.2 micron filter. The 16S rRNA gene was amplified by PCR with primers 515F (Parada) and 926R (Quince) with attached Illumina adapters containing barcodes and index sequences following the setup described in Parada et al. 2016, doi:10.1111/1462-2920.13023.
SUB15408556
Lt.A.CF