16S rRNA amplicon sequencing of Sclerodactyla briareus, background seawater sample
Microbial cells from seawater was collected on a 0.2 m filter via vacuum filtration, and DNA was extracted from the filter. The 16S rRNA gene was amplified by PCR with primers 515F (Parada) and 926R (Quince) with attached Illumina adapters containing barcodes and index sequences following the setup described in Parada et al. 2016, doi:10.1111/1462-2920.13023.
SUB15408556
Sb.water