GSM9115241: FFA2 fl/fl control mice sample 3; Mus musculus; RNA-Seq
Mature adipocytes were isolated from perigonadal adipose tissue via collagenase digestion. Total RNA was extracted from isolated adipocytes using TRIzol Reagent (Life Technologies) following the manufacturer's instructions. Phase separation was performed using chloroform, and the aqueous RNA phase was recovered for downstream processing. RNA libraries were constructed using the Illumina TruSeq Stranded mRNA Library Prep Kit, which includes poly-A selection to enrich for mRNA. Libraries were sequenced on an Illumina NovaSeq X Plus system using paired-end 150 bp reads according to manufacturer protocols.
GEO
GSM9115241_r1