GSM8833974: PFD; Mus musculus; RNA-Seq
In vitro samples (scRNA-seq): cultured cells were dissociated using accutase for 3 min (STEMCELL Technologies), washed with 1X DPBS (Gibco), strainer-filtered (40 and 70 µm Flowmi) to remove debris, and re-suspended in 0.04% BSA. In vivo samples (snRNA-seq): snap-frozen apical tissue slices were cut into smaller pieces (< 1 mm^3) on a pre-cooled surface on ice using a clean cold razor, and gently homogenized over two rounds using a Dounce homogenizer. The isolated nuclei were then strainer-filtered (40 and 70 µm Flowmi) to remove debris and re-suspended in Diluted Nuclei Buffer (10X Genomics). After single-cell/nucleus isolation, libraries were constructed following exactly the instructions in the User Guides provided by the manufacturer: 10X Chromium Single Cell 3' v3.1 protocol for the in vitro samples, and 10X Chromium Single Cell Multiome ATAC + GEX (the snRNA-seq GEX portion only) for the in vivo samples.
GEO
GSM8833974_r1