RNA-Seq of human bronchial cells (BEAS-2B) treated with 4-40 µg/mL Cu by CuO NP and ionic Cu, and human hepatoblastoma cells (HepG2) treated with 4-40 µg/mL ionic Cu compared to untreated controls
RNA-Seq of human bronchial cells (BEAS-2B) treated with 4-40 µg/mL Cu by CuO NP and ionic Cu, and human hepatoblastoma cells (HepG2) treated with 4-40 µg/mL ionic Cu compared to untreated controls
Both cell lines were first washed with warm PBS. BEAS-2B cells were then treated with 700 µL Accutase(R) for 3 min in a humidified incubator, and cell suspension was transferred to a 1.5 mL microtube. HepG2 cells were treated with 0.25 % trypsin-EDTA in PBS for 3 min in a humidified incubator and reaction was stoppped with 700 µL DMEM clel culture medium supplemented with 10 % FBS. Cell suspension was then also transferred to a 1.5 mL microcentrifugation tube. For both cell lines, cell suspensions were centrifuged at 300 g at 4 °C for 5 min, and the cell pellet was washed with cold PBS three times.  4.5×10^5 cells were seeded in 6-well plates for 24 h in a humidified incubator. For BEAS-2B cells, wells were previously treated with a 1:1:1 mixture of 10 µg/mL fibronectin, 30 µg/mL collagen, and 10 µg/mL bovine serum albumin for 30 min at 37°C.  Cells were exposed to 0-40 µg/mL total Cu for 24 h. Cu exposure was performed using either CuO NP or CuCl2 in BEAS-2B cells, and with CuCl2 in HepG2 cells. The NucleoSpin RNA Plus kit from Marchery Nagel (Germany) was used and manufacturer's instructions were followed.  Samples were sent to NovoGene Munich. An mRNA-Sequencing was applied using Illumina RNA-Sequencing with poly-A capture and reverse transcription of mRNA into cDNA. 
European Bioinformatics Institute
E-MTAB-16794:HepG2_Ctrl_1_p