GSM9178389: FN coated dishes + BMM + IgG #3; Mus musculus; RNA-Seq
RNA was isolated using TRIzol reagent (Qiagen, #79306) according to manufacterer's instructions. RNA samples were sent to Novogene (Sacramento, California) to construct the cDNA libraries. Messenger RNA was purified and enriched from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dTTP. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and Bioanalyzer 2100 system (Agilent) for size distribution detection The clustering of the index-coded samples was performed on a cBot Cluster Generation System using PE Cluster Kit cBot-HS (Illumina) according to the manufacturer's instructions. After cluster generation, the library preparations were sequenced on an Illumina Novaseq X Plus on 25B Flowcell and paired-end reads were generated.
GEO
GSM9178389_r1