GSM8520377: C2C12, H3K4me3, ChIP; Mus musculus; ChIP-Seq
Cells were subjected to nuclei isolation, chromatin fragmentation using Micrococcal Nuclease and nucleosome purification by Hydroxyapatite. Immunoprecipitation was performed overnight at 4°C with 6μg of chromatin and 5μg of antibody previously incubated with Protein-A- (for H3K27ac, H3K4me3, H3K27me3 antibodies) or Protein-G-coated (for H3.3 antibody) magnetic beads. Library was prepared using KAPA Hyper Prep Kits with PCR Library Amplification/Illumina series (Roche, 07962363001) with IDT for Illumina TruSeq DNA-RNA UD 96 Indexes (UDI) (Illumina, 2023784) and quantified by RT-qPCR using NEBNext Library Quant Kit for Illumina (NEB, E7640AA). Sequencing was carried out on paired-end 50bp of Illumina HiSeq4000.
GEO
GSM8520377_r1