GSM9217621: MCF7, PNA21, rep3; Homo sapiens; miRNA-Seq
Cellular total RNA was purified using the miRNeasy Mini Kit (Qiagen) and a robotic workstation for automated purification (QIAcube, Qiagen) according to the standard program without optional DNase treatment. A library for RNA-seq analysis was prepared from 100 ng total RNA using QIAseq miRNA Library Kit for Thermo Fisher Scientific NGS Systems (Qiagen) according to the manufacturer's protocol. QIAseq miRNA 12 Index TF (Qiagen) was used to distinguish samples.
GEO
GSM9217621_r1