GSM9179267: ID_020; Homo sapiens; RNA-Seq
Separating one part of human GBM tissue, the RNA isolation was performed using the RNAiso Plus (TAKARA, 9109) according to the manufacturer's instructions. RNA quality was evaluated spectrophotometrically, and the RNA integrity was assessed with the Agilent 2100 Bioanalyzer (Agilent Technologies). RNA-seq libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, no.E7775). Once prepared, indexed cDNA libraries were pooled in equimolar amounts and sequenced with paired-end reads on an Illumina NovaSeq 6000 (Illumina).
GEO
GSM9179267_r1