Illumina NovaSeq X paired end sequencing of SAMD01688408
Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP. The strand specific library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.
Kagoshima University Graduate School of Medical and Dental Sciences
DRX730806