GSM9834814: MSK107Li PDO cells, shCtrl, no ActD, replicate2; Homo sapiens; OTHER
Total RNA was extracted from organoids using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. RNA concentration was measured using the RiboGreen assay, and RNA quality was assessed using an Agilent Bioanalyzer. 300 ng of total RNA with RIN values of 9.8-10 underwent polyA selection and TruSeq library preparation according to instructions provided by Illumina (TruSeq Stranded mRNA LT Kit, catalog # RS-122-2102), with 8 cycles of PCR.
GEO
GSM9834814_r1