GSM8553151: FXS_7, MeRIP; Homo sapiens; OTHER
Total RNA was isolated from six-week-old cortical neurons and postmortem cortex tissues using TRIzol reagent and Phase Lock Gel Heavy Purified mRNA samples were fragmented to 100-200 nucleotides.  10% fragmented RNA was set aside as an input sample, while the remaining RNA was used for m6A immunoprecipitation. The fragmented RNA was incubated with anti-m6A antibody and then with protein G magnetic beads. The m6A-enriched RNA was purified using TRIzol. The library was prepared following the instructions for Kapa RNA hyper prep kit. Both the input samples without IP and the m6A IP samples were subjected to 150-bp, paired-end sequencing on an Illumina NovaSeq 6000 sequencer.
GEO
GSM8553151_r1