GSM8899554: Bone marrow DAPI-lineage-cKit+ cells, Mx1-cre;Pex5fl/fl, Mutant 1; Mus musculus; RNA-Seq
Bone marrow cells were isolated by flushing long bones.The cells were passed through 25G syringes at least twice in Ca²⁺- and Mg²⁺-free HBSS containing 2% heat-inactivated bovine serum to obtain a single-cell suspension. The cells were filtered through a 70-μm nylon mesh.The following antibodies were used for staining cells (all from Biolegend): lineage markers (anti-Ter119, anti-B220 (6B2), anti-Gr1 (8C5), anti-CD2 (RM2-5), anti-CD3 (17A2), anti-CD5 (53-7.3) and anti-CD8 (53-6.7)), anti-Sca-1 (E13-161.7), and anti-cKit (2B8). DAPI-lineage-cKit+ cells from bone marrow  were double-sorted in a yield-purity order of sorting modes on BD SORP FACSAria. The sorted cells were resuspended in Ca²⁺- and Mg²⁺-free HBSS. 10x Genomics Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 were used to prepare the library according to the manufacturer's instructions.
GEO
GSM8899554_r1