MinION of soil microbiome (ITS): rhizosphere of Cucurbita pepo
The metagenomic DNA from each soil sample was extracted using the DNeasy PowerSoil Pro Kit for soil from QIAGEN, following the manufacturers instruction. DNA concentration was determined by Nanodrop (Thermo Fisher). The ITS1-5.8S, ITS2 and D1/D2 domain of 26S subunit (Primers: ITS1 5-TCCGTAGGTGAACCTGCGG; NL4 5-GGTCCGTGTTTCAAGACGG) were amplified. Library preparation was performed according to the methods of Conti et al. (2022).
SUB14729703
Sp_rhizo_ITS_2