Pathogen: clinical or host-associated sample from Norovirus GI.1
RNA was isolated from stool samples or infected human intestinal enteroids (HuNoV) followed by Real-Time RT-PCR to detect these viruses. Positive samples were quantified, and RNA was converted to cDNA. The cDNA was used to generate Illumina libraries with molecular barcodes and these libraries were pooled based on the Ct. values. Capture enrichment was performed with either HuNoV probe set, and enriched libraries were then sequenced on the Illumina NovaSeq 6000 instrument to generate 2x150 bp length reads.
SUB15411355
p1336-100874