GSM8553304: K-SZ-4wk-B; Mus musculus; RNA-Seq
Total islet mRNA was extracted using RNeasy Kit (Qiagen, Germantown, MD) RNA quality was initially assessed using Qubit fluorometry (ThermoFisher Scientific, Waltham, MA) and the Agilent Fragment Analyzer (Santa Clara, CA). cDNA libraries were prepared using 150 ng of total RNA according to the manufacturer's instructions for the TruSeq Stranded mRNA Sample Prep Kit (Illumina, San Diego, CA). The concentration and size distribution of completed libraries were determined using an Agilent Bioanalyzer DNA 1000 chip and Qubit fluorometry. Libraries were sequenced at six samples per lane following Illumina's standard protocol using the Illumina cBot and HiSeq 3000/4000 PE Cluster Kit. The flow cells were sequenced as 100 x 2 paired end reads on an Illumina HiSeq 4000 using HiSeq 3000/4000 sequencing kit and HD 3.4.0.38 collection software. Base-calling was performed using Illumina Real Time Analysis version 2.7.7.
GEO
GSM8553304_r1