GSM8864973: PM_rep2; Mus musculus; RNA-Seq
Total RNA was extracted from frozen hippocampus tissue of mice using the PureLink RNA Mini kit according to the manufacturer's instructions (Thermo Fisher Scientific). The concentration and quality of RNA were assessed by an Agilent Bioanalyzer 2100 (Agilent Technologies, CA). Ribosomal RNA was removed from 1 ug of total RNAs with more than RIN value 8.0 and cDNA was synthesized using SuperScript III (Invitrogen). Libraries for sequencing were prepared according to the manufacturer's instructions with NEBNext Ultra II RNA Library Prep Kit (New England Biolabs, NEB#E7775) and 100bp paired-end sequencing was done with a NovaSeq 6000 instrument (Illumina). Libraries for sequencing were prepared using standard New England Biolabs's protocols.
GEO
GSM8864973_r1