Mitogenome sequencing of Lutjanus rufolineatus
Three pairs of mitochondrial-specific primers were designed based on the conserved regions of existing Lutjanus species and were used to amplify target regions via long-range PCR. Amplicons were sequenced on the Illumina NextSeq1000 platform,  assembled with SPAdes Genome Assembler v.4.1.0, and functionally annotated using MITOS2 v.2.1.9. web server and NCBI BLAST
SUB15523255
PGCMIN_NS039_FISH_F2