GSM9002194: BMP_R1; Owenia fusiformis; RNA-Seq
Total RNA from these cultures were extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs; Cat No.: #T2010) mRNA fraction were selected from the total RNA before converted to cDNA. Second strand cDNA synthesis had dUTP incorporated. The cDNA was end-repaired, A-tailed and adapter-ligated. Prior to amplification, samples underwent uridine digestion. The prepared libraries were selected, multiplexed and QC'ed before paired-end sequenced over 1 of a NovaSeq v1.5 flow cell.
GEO
GSM9002194_r1