GSM8517770: 2MS_1; Quercus robur; RNA-Seq
Samples  were collected at 0, 1, and 2dpi, by snap-freezing mock and infected leaves in liquid nitrogen, and stored  at -80°C until processing. Material was collected from 4 biological replicates (plants) per group giving a total of 80 samples for each omics analysis. Total RNA was extracted using the Mini-Plant RNA extraction kit following manufacturer instructions with modifications for leaf tissue. Briefly, a 1:1 combination of PL1 and PL2 was applied during the lysis step. Centrifuge times were duplicated during the extraction and two additional washing steps were incorporated. RNA quality was tested with nanodrop and gel electrophoresis. Library preparation and sequencing was conducted by Novogene using NovaSeq 6000 PE150. Around 40 million paired reads were generated per sample.
GEO
GSM8517770_r1