GSM8972939: 786-O HA-HLF ChIP Rep2; Homo sapiens; ChIP-Seq
Samples were then lysed in lysis buffer (10mM Tris-HCl pH 8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 1% Triton x-100) and sonicated by using the BioRuptor. Dynabeads were incubated with FLAG or HA antibody at 4°C overnight with rotation, followed by incubation with sonicated samples overnight. Then the samples were washed and collected by applying a magnetic stand, eluted and reverse crosslinked. RNase A (sigma, 70856-3) was used for RNA degradation, and Proteinase K (sigma, 3115828001) was used for protein degradation. DNA was purified by using Qiagen PCR purification kit (Qiagen, 28106). DNA libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit (NEB, E7645S), followed by followed by sequencing at the CRI's Sequencing Facility at UT Southwestern.
GEO
GSM8972939_r1