GSM9694742: Western diet, brown adipose, Sphk2 deleted repicate 1; Mus musculus; RNA-Seq
RNA was extracted from equal weights of frozen tissue using TRIzol (#15596026, Fisher). 1ug of total RNA was used for construction of sequencing libraries. RNA libraries for RNA-seq were constructed from poly(A) isolated RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (#E7490S/L, New England BioLabs) and enriched with a SMARTer Apollo Library Prep System (#640078, Takara). The library was prepared using a dUTP-based stranded NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (#E7760S, New England BioLabs), indexed and amplified with 8 PCR cycles. Uniquely barcoded libraries were then analyzed and sequenced with the paired end read 2x61 base pair setting.
GEO
GSM9694742_r1