RNA-seq of mouse
DNA concentration was determined using a Qubit 4 Fluorometer (Thermo Fisher Scientific), and library size and quality were assessed on an Agilent 2100 Bioanalyzer (Agilent Technologies) using the DNA High Sensitivity kit. Sequencing was performed on an Illumina MiSeq platform (Illumina, San Diego, CA, USA) with a 2  300 bp paired-end configuration. Library pool quantification was performed by qPCR using a QuantStudio 5 Real-Time PCR System (Applied Biosystems).
SUB15540815
16S-lib10-SV-02