RNA-Seq of RKO (human) cells treated with CPT 2.5uM for 10h (rep1)
RKO cells were treated with CPT (2.5M) with or without PARPi (Talazoparib, 2.5M) and ATMi (10 M) for 10 h, and total RNA was extracted using the Quick-RNA MiniPrep Kit (Zymo #11328). Poly(A) RNAs from five micrograms of total RNA for each sample were then selected using NEBNext Poly(A) mRNA magnetic isolation module (#E7490L) and were then used to construct the sequencing library using Invitrogen Collibri Stranded RNA Library Prep Kit (Thermo Fisher Scientific, #A39114096), according to the manufacturers protocols. Libraries were amplified using 12 PCR cycles. Library size distributions were measured using a BioAnalyzer and quantified through qPCR. Libraries were sequenced on a NovaSeq X Plus platform.
SUB15792205
CPT2.5uM_10h_rep1