HiC sequencing of G. inflata (CRX1917320; CRA019063)
For Hi-C library construction and sequencing, the leaves of G. inflata was crosslinked using 4% formaldehyde solution. After quenching the crosslinking reaction, the digestion reaction was digested with the restriction enzyme Dpn II. Then, the ends of fragments were marked with biotin and the DNA fragments were decrosslinked. After adding sequencing adapters to the fragment ends, Hi-C library was amplified by PCR and sequenced on Illumina HiSeq platform submitted_instrument_model: Illumina HiSeq X Ten
NGDC
CRX1917320
South China Botanical Garden, Chinese Academy of Sciences
DRX803012