GSM9769662: alx3 Mutant 48hpf CNCCs; Danio rerio; RNA-Seq
33 double transgenic fli1:EGFP;sox10:mRFP embryos per genotype were dissociated into single-cell suspension using cold-active protease from Bacillus licheniformis, DNase, EDTA, and trituration. Sorted cells (at 3.27 x 105 cells/ml in wild type, at 2.72 x 105 cells/ml in alx3-/-, and at 2.86 x 105 cells/ml in alx3-/-;alx4a-/- with ~85% viability) were loaded into the 10X Chromium Controller aiming to capture > 10,000 cells per sample. 2x150 paired-end sequencing Chromium 10X Genomics libraries were sequenced using the NovaSEQ6000.
GEO
GSM9769662_r1