Pacbio HiFi data of Aurelia coerulea (Aco2019JZB02)
High-molecular-weight genomic DNA was extracted from a single Aurelia coerulea individual. DNA quality was assessed by Qubit fluorometer and pulsed-field gel electrophoresis. DNA was sheared to ~15 kb using a Covaris g-TUBE, followed by end-repair and size selection with BluePippin (Sage Science). PacBio HiFi circular consensus sequencing (CCS, long-read sequencing, library strategy: PacBio HiFi) was performed on a PacBio Sequel II system. The resulting HiFi reads were used for de novo genome assembly with hifiasm, and HiFi reads were mapped to the assembled Aurelia coerulea genome (AcoGenome.fasta) for assembly evaluation and curation.
SUB15431427
AcoPacBio