WGS_PAO1_laboratoryEvolution(Start strain: PA01)(Evolution drug: Colistin)(Evolution medium: Lactate single nutrient)(Evolution days: 10)(Bioreplicate: 3)
500  of the cell suspension was pelleted (10 minutes centrifugation at 5000 x g). DNA extraction was performed by the QIAcube Connect automated sample preparation system using Qiagen DNeasy Blood & Tissue Kit extraction kit. Illumina DNA Prep tagmentation kit was used to prepare sample for WGS. Sequencing was performed on the Illumina NextSeq2000 platform using a 300 cycle flow cell kit. Read demultiplexing, read trimming, and run analytics were performed using DRAGEN v3.10.12.
SUB15049715
seq045