GSM9474280: CK1; Aspergillus niger; RNA-Seq
(1) cytology: the concentration of cells is adjusted to the desired concentration, usually 700-1200 cells/ΜL. (2)10X labeled cDNA fragments: gel beads containing barcode information bind to a mixture of cells and enzymes and are then coated with Surfactant droplets located in a microfluidic system to form GEMs. Gems flow into the reservoir and are collected, the gel beads dissolve to release Barcode sequences, cDNA fragments are reverse transcribed, and samples are labeled. The gel beads were broken and the oil droplets were broken, and the cDNA was used as a template for PCR amplification. The products of all GEMs were mixed to construct a standard sequencing library. Construction of mRNA library: DNA library was constructed by cutting cDNA into 200 ~ 300 BP fragments, sequencing adapters and primers, and PCR amplification.
GEO
GSM9474280_r1