GSM8834892: DTR_Positive; Mus musculus; RNA-Seq
Mouse lymph nodes (mesenteric, cervical, brachial, axillary, inguinal, and para-aortic) were procured and pooled in digestion medium consisting of RPMI 1640 with 2% foetal bovine serum (FBS) (Sigma-Aldrich), with deoxyribonuclease (DNase) (100 µg/mL; Roche) and LiberaseTM (96 µg/mL; Roche), minced and agitated at 37°C with gentle agitation for 30 min, and passed through a 70-micron filter. Cells were then sorted for live, tdTmt+ MHCII+ cells and processed for single-cell analysis. 10x Chromium V3 3' Protocol
GEO
GSM8834892_r1