Sc-TK2_S288_L001 (CRX1856365; CRA027871)
The gel beads containing barcode information combine with the mixture of cells and enzymes, enter the reservoir and are separated by oil to form GEMs. The gel beads dissolve and release the captured sequence containing Barcode sequence, reverse transcribe the cDNA fragment, and label the sample. The gel beads were broken and the oil drops were broken, and the cDNA was used as the template for PCR amplification. Mix all GEMs products and construct a standard sequencing library. Cut the cDNA enzyme into fragments of about 200-300 bp, and then perform conventional second-generation sequencing library construction steps such as end repair, A-tail addition, sequencing adapters P5, P7, and sample index. Finally, PCR amplification is performed to obtain the DNA library. submitted_instrument_model: Illumina HiSeq X Ten
NGDC
CRX1856365
South China Agricultural University
DRX797758