GSM9580081: lung, PVC1 treated, 2 months,rep1; Mus musculus; RNA-Seq
Lung tissues were dissociated into single-cell suspensions using sCelLive™ Tissue Preservation Solution, Singleron Python™ Automated Tissue Separator, and sCelLive™ Tissue Isolation Mix according to the manufacturer's instructions Raw reads were processed using FastQC (version 0.11.4) for quality control. Low-quality reads were removed using fastp. Poly-A tails and adapter sequences were trimmed with Cutadapt (version 1.18). Reads were aligned to the GRCm38 (mm10) reference genome using STAR (version 2.5.3a) with default parameters. Gene counts were generated using featureCounts (version 1.6.2), with a UMI threshold of 30,000.
GEO
GSM9580081_r1