GSM8864183: A549 cells, vehicle, mock, 8h, rep2; Homo sapiens; RNA-Seq
Total RNA was isolated from samples using NAxtrakit following the manufacturer's protocol at 8 hours (A549 cells) or 24 hours (retinal organoids) post-infection. The quality and quantity of extracted RNA were evaluated prior to library preparation. mRNA was enriched from total RNA using oligo-dT beads to select for polyadenylated transcripts. The enriched mRNA was fragmented into smaller pieces, which were used for cDNA synthesis. First-strand cDNA synthesis was performed using random primers, followed by second-strand synthesis incorporating dUTP in place of dTTP. The resulting cDNA fragments were end-repaired and adenylated at the 3′ ends, after which adaptors were ligated. Libraries were then amplified via polymerase chain reaction (PCR) and assessed for quality. The amplified libraries were circularized and further amplified to produce DNA nanoballs (DNBs). Sequencing was performed on the DNBSEQ Technology platform with a paired-end read length of 150 bp (PE150), using the DNBSEQ Eukaryotic Strand-specific mRNA library.
GEO
GSM8864183_r1