RNA sequencing of non–small cell lung cancer (NSCLC) cell line H1299 following chloroquine (CQ) treatment
RNA sequencing of non–small cell lung cancer (NSCLC) cell line H1299 following chloroquine (CQ) treatment
3X105 H1299 cells were cultured in RPMI-1640 medium containing 10% FBS for 24 hours. The medium was then replaced with fresh RPMI-1640 supplemented with 1% FBS, 5 mM glucose, 4 mM glutamine, and 10 µM CQ for an additional 24 hours. H1299 cells were maintained in RPMI-1640 medium supplemented with 10%FBS and in humidified atmosphere with 5%CO2 at 37°C. RNA was harvested using Rneasy mini plus kit (Qiagen), 2 µg of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using SMARTER Mrna-SEQ Library Prep Kit following manufactures's protocols.
European Bioinformatics Institute
E-MTAB-16126:CQ-1b_p