GSM9836130: Mus musculus, epididymal WAT, Submissive (Sub) mouse, STD, biological replicate 4; Mus musculus; RNA-Seq
Adipose tissues (iWAT and eWAT) were dissected, snap-frozen in liquid nitrogen, and stored at -80°C. Total RNA was extracted using RNeasy Lipid Tissue Mini Kit (Qiagen). RNA quality was assessed using TapeStation 4200 (Agilent). RNA-seq libraries were constructed using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, cat. E7760). 250 ng total RNA was used. mRNA pull-down was performed using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, cat. E7490). Library QC was performed using Qubit (Invitrogen) and TapeStation 4200 with High Sensitivity D1000 kit. Sequencing was performed on Illumina NextSeq2000 using P2 100 cycles (Read1-100; Index1-8; Index2-8) at the Technion Genomics Center, Haifa, Israel. Libraries were sequenced as single-end 100 bp reads.
GEO
GSM9836130_r1