GSM9083530: HEK293T SINV-infected cells smallRNA-seq replicate 1; Homo sapiens; ncRNA-Seq
Total RNA was extracted from cell lysates using Trizol Reagent (15586018, InVitrogen) to which 200 µl of chloroform was added before centrifugation for 15 minutes at 12,000 xg at 4°C. For small RNA extraction, supernatants were collected and nucleic acids were precipitated in 3 volumes of 100% ethanol + 10% 1M sodium acetate for 30 minutes at -80°C. RNA was pelleted for 10 min at 12,000 xg at 4°C then washed with 70% ethanol and reconstituted in RNase free water. Samples were shipped to BGI for library preparation. Sequencing was realized on DNBSEQ technology, for 50bp single-end reads and standard output of 20 million reads per sample.
GEO
GSM9083530_r1