GSM8695102: hLUC7L3 overexpression replicate 3; Homo sapiens; RNA-Seq
Cell line: RNA was extracted 24 hours after transfection using the Qiagen RNeasy Mini kit (cat. no. 74104) according to the manufacturer's instructions with the optional on-column DNase digestion (cat. No 79254). Seedlings: Seedlings were collected and flash frozen in liquid nitrogen. Total RNA was isolated using RNeasy® Plant Mini Kit (Qiagen, cat. nos. 74904) according to the manufacturer's instructions. Cell line: Illumina-compatible libraries were prepared by MIT BioMicroCenter using NEB II Ultra Directional RNA with poly(A) selection and sequenced on NovaSeq 6000 with 2 x 150 bp reads. Seedlings: mRNA stranded library preparation and sequencing (PE150) was done by Novogene (Cambridge, United Kingdom) using an Illumina Novaseq6000 system.
GEO
GSM8695102_r1