NP1 (CRX2216747)
Total RNA was extracted from cabbage leaf tissues using a combination of the CTAB method and the pBIOZOL Plant Total RNA Extraction Reagent. The RNA integrity was assessed using the Qsep400 High-throughput Nucleic Acid Analysis System. Following quality control, libraries were constructed using the VAHTSTM mRNA Capture Beads 2.0 to enrich polyA-tailed mRNA. The enriched mRNA was fragmented, and first-strand cDNA was synthesized using random hexamer primers. During second-strand synthesis, dUTP was incorporated instead of dTTP for strand-specificity. The library underwent end repair, A-tailing, and adapter ligation. Fragments of 250-350 bp were selected using VAHTSTM DNA Clean Beads. The final strand-specific libraries were prepared on an MGISP-960 automated system and sequenced on the DNBSEQ-T7 platform to generate 150 bp paired-end reads. This experimental design includes three biological replicates for each of the three shading treatment groups (high-shading, low-shading, and no-shading).
Institute of Horticulture, Guizhou Academy of Agricultural Sciences
DRX1012180
NGDC
CRX2216747