DNAseq of Cryptococcus neoformans H99 crossed with C. deneoformans JEC20
To analyse the effect of Spo11 during hybridization between C. neoformans and C. deneoformans, we conducted two types of crosses: H99 (C. neoformans, wild type) x JEC20 (C. deneoformans, wild type) and H99 spo11d::NAT x JEC20 spo11d::NAT, and recovered random spores by microdissection following protocols published previously. Spore germination rates were calculated and then 6 large and 6 small colonies from each cross were patched, from which genomic DNA was purified and sequenced at the Duke Sequencing and Genomic Technologies core facility (https://genome.duke.edu), on a Novaseq X platform with 250 bp paired-end option.
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Progeny23