Single Cell RNAseq of C57Bl/6J mice: CD70KO; Weight Loss; Adipose Tissue
Sample preparation for obese and weight cycled samples was conducted using the 5 assay for the 10X Chromium platform (10X Genomics) targeting 20,000 cells per diet group. In all, 50,000 reads per cell were targeted for PE-150 sequencing on an Illumina NovaSeq6000. The Chromium Next GEM Single Cell 5 HT v2 kit from 10X Genomics was implemented for both lean and WL samples and run on the NovaSeq 6000. All samples were run at the VUMC VANTAGE core facility.
SUB14958125
11566-JG-5