TCR Amplicon of human: Acute SARS-CoV2 patient's PBMCs
Bulk-TCRseq: RNA from acute SARS-CoV2 patient's PBMCs reverse transcribed using TSO with UMIs and TCR gene specific C-segment primers (Egorov et al. 2015). cDNA amplified using TCR constant segment and TSO-specific primers. Libraries made using KAPA Hyperprep kit and sequenced on illumina NovaSeq.
SUB15341995
BulkTCR_F2_tp2_alpha