RNA-Seq of human brainstem tissue: Mycobacterium tuberculosis positive sample
Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. Following fragmentation, first-strand cDNA was synthesized using random hexamer primers, followed by second-strand cDNA synthesis. Libraries were generated through end repair, A-tailing, adapter ligation, size selection, amplification, and purification. Libraries were sequenced in paired-end mode on an Illumina NovaSeq X Plus platform.
SUB15937495
Mtb_positive2_L1