GSM8659453: colon, PSC-UC, replicate 4; Homo sapiens; Bisulfite-Seq
Unstained 5×5 µm sections were serially deparaffinized in xylene, followed by serial dehydration in ethanol and rehydration in water. DNA/RNA extractions were performed using the Norgen dual nucleic acid extraction FFPE kit (Norgen Biotek Corp.) according to the manufacturer's instructions. DNA was quantified using the Qubit High Sensitivity quantification kit, and RNA was quantified using a NanoDrop 2000 (Agilent Technologies Ltd.). DNA libraries were generated using the Kapa DNA HyperPrep kit (Kapa Biosystems), followed by bisulfite conversion using the Zymo DNA Methylation Lightning kit (Zymo Research). Sequence capture was performed with the Roche SeqCap Epi sequencing capture kit using custom-designed probes. Libraries were sequenced on the Illumina NovaSeq 6000 platform (2×150 bp).
GEO
GSM8659453_r1