RNA-seq of different tissues from wild-type and C/EBPα ΔuORF mutant African turquoise killifish Nothobranchius furzeri
RNA-seq of different tissues from wild-type and C/EBPα ΔuORF mutant African turquoise killifish Nothobranchius furzeri
Whole fish liver, brain, skin and muscle samples were harvested from three NfCEBPAΔuORF animals and three wild-type animals. Total RNA was isolated using TRIzol™ Reagent (Invitrogen) according to the manufacturer's protocol. RNA-seq libraries were prepared according to the Smart-3SEQ protocol (https://doi.org/10.1101/gr.234807.118) using 40 ng of total RNA per sample. The first read starts with 6 nt UMI, followed by a spacer of 0,3 or 6 nt, followed by GGG and the RNA sequence. The second read starts with 6 nt UMI.
European Bioinformatics Institute
E-MTAB-15325:Muscle_WT_22-117_p