chloroplast genome of A. densiflorusSprengeri: young leaf
Plant samples of A. densiflorus 'Sprengeri' were collected from Weifang, China. Fresh leaves were packaged in thin foil, then frozen by liquid nitrogen for high throughput sequencing. Genomic DNA was extracted using a DNA easy Plant Mini Kit (Qiagen Co., Hilden, Gemany) following the manufactures instructions. NanoDrop2000C spectrophotometry and electrophoresis in 1% (w/v) agarose gel were used to detect the concentration and integration of the total DNA, respectively. Extracted DNA was fragmented to an average size of approximately 400 bp using CovarisM220 (Gene Company Limited, China) for paired-end library construction. Paired-end library was constructed using NEXTFLEX Rapid DNA-Seq (BiooScientific, Austin, TX, USA). Adapters containing the full complement of sequencing primer hybridization sites were ligated to the blunt end of fragments. Paired-end sequencing was performed on Illumina NovaSeq platform (Illumina Inc., San Diego, CA, USA) at Majorbio Bio-Pharm Technology Co., Ltd. (Shanghai, China). Raw reads were quality controlled with Trimmomatic and Fast QC software (https://www.bio- informatics.babraham.ac.uk/projects/fastqc). Clean reads were then assembled using GetOrganelle with parameter -R10 -k 21, 45, 65, 85, 105, 121 -Fembplant_pt (Jin, et al. 2020).
SUB15333973
A. densiflorus