OsCGA1_cuttag-1 (CRX2203817)
Plants aged 46 to 49 days were harvested for CUTTag assays. CUTTag libraries were prepared using the Hyperactive Universal CUTTag Assay Kit for Illumina Pro (Vazyme, TD904) following the manufacturer's protocol. Purified nuclei were bound to ConA coated magnetic beads, permeabilized with Digitonin, and incubated overnight  with anti-Flag primary antibody (M20008, Abmart). After washing, a secondary anti-mouse IgG antibody (Ab208, Vazyme) was applied for 1 h at room temperature. Tagmentation was performed by adding Protein AorG Tn5 transposase,  PCR-amplified (16 to18 cycles) with indexed primers (TruePrep Index Kit, TD207, Vazyme). and were purified with VAHTS DNA Clean Beads (Vazyme, N411), and  sequenced on an Illumina NovaSeq 6000 platform to generate 150-bp paired-end reads.
CAS Center for Excellence in Molecular Plant Sciences / Institute of Plant Physiology and Ecology
DRX1011989
NGDC
CRX2203817