GSM9083860: PASMCs-hox_1; Mus musculus; RNA-Seq
For RNA-seq, RNA was isolated by miRNeasy micro Kit (Qiagen) combined with on-column DNase digestion (RNase-Free DNase Set, Qiagen) to avoid contamination by genomic DNA. RNA and library preparation integrity were verified with LabChip Gx Touch (Perkin Elmer). RNA amounts were normalized and all 10ng of total RNA was used as input for SMART-Seq® v4 Ultra® Low Input RNA Kit following manufacture's protocol (Takara Bio). Sequencing was performed on the NextSeq2000 platform (Illumina) using P3 flowcell with 72bp single-end setup.
GEO
GSM9083860_r1