16r RNA-Seq of rat: gut
Two fecal samples, one collected before pregnancy and the other collected during the 3rd trimester of pregnancy (median: Day19, range: Day15-19) (Figure 1), were analyzed for each rat. Fecal DNA was extracted using a Qiagen fecal DNA extraction kit (Qiagen). Sierragenomics (San Mateo, CA) performed Bacterial 16S rRNA gene library preparation and NGS sequencing (Sierragenomics, San Mateo, CA). The hypervariable regions of the 16S rRNA gene (16S rDNA, the V4 region) were amplified using PCR primers. The amplicons were sequenced on the Illumina MiSeq machine.
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FF3-A