Whole genome from bacterial strain grown on PA
The genomic DNA was randomly sheared into shorter fragments. The obtained fragments were then end-repaired, A-tailed, and further ligated with Illumina adapters. The resulting fragments with adapters were size selected, PCR amplified unless otherwise specified as PCR-free, before proceeding for purification.The library was quantified through Qubit and qPCR, and size distribution detected with fragment analyzer. Quantified libraries were pooled and sequenced on Illumina platforms according to the effective library concentration and required data amount.
SUB15304321
MPA_1_3_B