GSM9115179: DSP-1001660038290-A-D06, Castration-, DLP, Treatment-, Mouse 416; Mus musculus; OTHER
﻿Murine prostate samples were harvested and fixed in 4% PFA, then paraffin wax-embedded. 5 µm sections were cut, and baked for 30 min at 60 °C. The nuclei and the epithelial cells were stained using SYTO™ 83 Orange Fluorescent Nucleic Acid Stain (S11364, Thermo Fischer) and the Alexa Fluor 488 anti-mouse CD236 (Ep-CAM) antibody (#AB237384, dilution 1/100, Abcam), respectively. In-situ hybridisation of RNA-directed DNA oligo probes (Nanostring Mouse Whole Transcriptome Atlas) was performed according to the manufacturer's protocol. For each mouse, the EPCAM+ cells in 5 regions of interest (ROI) in the DLP and 5 in the AP were selected. Sequencing libraries were generated following the Nanostring GeoMx NGS protocol
GEO
GSM9115179_r1