GSM8766920: Liver, cell line, untreated, control, early time point; Homo sapiens; RNA-Seq
At respective time points, cells were trypsinized for 10 min, gently resuspend, and singularized by pipetting three times through a 70 µm cell strainer. Single-cell suspension were resuspended in appropriate volumes of 0.04% BSA/PBS, counted, and loaded onto the 10x Chromium controller according to manufacturer's instructions. The targeted cell recovery was 5,000. Libraries were constructed and pooled accrording to the manufacturer's instructions of the Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (Dual Index) Kit.
GEO
GSM8766920_r1