Targeted sequencing of C. atrox metalloproteinase gene complex
A hybridization-capture approach was used to enrich for genomic DNA encoding the metalloproteinase gene complex from individual specimens. Using the C. atrox metalloproteinase reference sequence (~1.2 mega base pairs (M bp) with 30 annotated MP genes) as a template, deoxyribonucleic acid (DNA) baits (100 nucleotides long) were designed and synthesized (Arbor Biosciences) at an average density of one bait every 125 nucleotides. The Pacific Biosciences Multiplex Genomic DNA Target Capture Using SeqCap-EZ Libraries protocol was followed for generating the final sequencing libraries. Kapa HiFi polymerase was used for all amplification steps. The average length of input genomic DNA fragments for the sequencing libraries was seven kilo basepairs (kb) (range of two - ten kb). The mean DNA fragment length of the initial barcoded library was five kb and the mean fragment length of the final enriched DNA library was four kb. The libraries were pooled and sequenced with the PacBio Sequel II.
SUB14633695
atx021dv_DNA