WGS resequencing of Methylobacterium symbioticum strain SB0023/3 genome
Illumina sequencing was carried in the lab. Library wasprepared using total DNA isolated from the overnight culture in R2A medium using the genomic DNA extraction with the Bacterial & Yeast Genomic DNA Purification Kit (EURx, Gdansk, Poland) according to the manufacturers instructions. The sequencing libraries were prepared using the Native Barcoding Kit 24 v14 (Oxford Nanopore Technologies) according to the manufacturers protocol, and sequencing was performed on an R10.4 flow cell with a MinION Mk1B device. The basecalling was performed with dorado v0.9.0 using dna_r10.4.1_e8.2_400bps_sup@v5.0.0 model.
SUB15722424
GoBioMs_ont