CRISPR-screening of Homo sapiens, high_CXCR4
A CRISPR screeningbased experimental strategy was employed. HUES8 cells were transduced with a metabolism-related sgRNA library delivered by lentivirus, followed by differentiation toward definitive endoderm. Cell populations representing the top 30% and bottom 30% of CXCR4-positive fractions were isolated and subjected to sequencing.
SUB15855038
V1_Pos.fq