GSM9798345: LCMV D30 post-transduction with stimulation central memory rep 31; Mus musculus; RNA-Seq
T cell populations were sorted on a Sony cell sorter into 96-well plates at 1,000 cells per well in lysis buffer consisting of TCL buffer supplemented with 1% 2-mercaptoethanol, then frozen before RNA capture and cDNA synthesis. RNA was isolated using SPRI beads and eluted directly into oligo-dT/dNTP-containing reverse-transcription mix. Full-length cDNA was generated using the Smart-seq2 protocol with SuperScript II reverse transcriptase, a template-switching oligonucleotide, and RNase inhibitor. cDNA was amplified by whole-transcriptome amplification using KAPA HiFi HotStart ReadyMix, purified with SPRI beads, quantified, and assessed by Bioanalyzer or TapeStation. Sequencing libraries were prepared from amplified cDNA using Nextera XT tagmentation with indexed adapters, pooled, and sequenced on Illumina NextSeq platforms at the Broad Institute Genomics Platform.
GEO
GSM9798345_r1