GSM8489979: veh_3 [ATAC]; Mus musculus; ATAC-seq
50,000 cells were centrifuged at 500 g for 5 min at 4°C and washed with PBS. The cell pellet was resuspended in 50 µl lysis/transposition reaction (12.5 µl THS-TD-Buffer, 2.5 µl Tn5, 5 µl 0.1% digitonin and 30 µl water) and incubated at 37°C for 30 min with occasional snap mixing. DNA fragments were purified by MinElute PCR Purification Kit (Qiagen). Library fragments were amplified together with indexing primers (1× NEBnext PCR master mix and 1.25 μM of custom Nextera PCR primers 1 and 2).
GEO
GSM8489979_r1