Habitat use and pathogens diversity in wild white-lipped peccary (Tayassu pecari) from the Osa Peninsula (Costa Rica)
The fecal samples were collected from wild white-lipped peccaries (Tayassu pecari) in the Rancho Quemado and Pochotal areas of the Osa Peninsula, Costa Rica. Samples were immediately frozen at -20 C. For viral metatranscriptomic analysis, samples were pooled by mammal species. Pooled samples underwent centrifugation (12,000g for 20 min at 4 C) to remove eukaryotic and bacterial cell-sized particles, followed by filtration through a 0.45-m filter to enrich for viral particles. Filtrates were digested with DNase and RNase (37 C for 60 min). Total nucleic acids were extracted using the QIAamp MinElute Virus Spin Kit (Qiagen). Enriched viral nucleic acid preparations were reverse transcribed using Super-Script IV (Invitrogen) and 100 pmol random hexamer primers, followed by a single round of DNA synthesis. Sequencing libraries were constructed using the Nextera XT RNA Sample Preparation Kit (Illumina) and sequenced as 250 bp paired-end reads on an Illumina MiSeq platform
SUB15503488
CostaRicaWildlifePoolL_S50