ChIP-Seq for H3K27ac landscape in doxorubicin-resistant MDA-MB-231
ChIP-Seq: MDA-MB-231 doxorubicin-resistant cells were fixed with 1% formaldehyde for 10 min at RT. ChIP-Seq: MDA-MB-231 doxorubicin-resistant cells were fixed with 1% formaldehyde for 10 min at RT. H3K27ac was immunoprecipitated with anti-H3K27ac antibody (Cell Signaling Technology Cat# 4353, RRID:AB_10545273) as previously described (doi: 10.1016/j.biopha.2023.115798). Library for sequencing was prepared using MGIEasy Cell-free DNA Library Prep Set and sequenced on MGI DNBSEQ-G400 using DNBSEQ-G400RS High-throughput Sequencing Kit from MGI
SUB15192299
ACMD