GSM8972844: mus skin,MV,rep4; Mus musculus; RNA-Seq
Total RNA was extracted by mjzol animal RNA isolation kit (major ivd) and purified by RNAClean XP Kit (Beckman Coulter) and RNase-Free DNase Set(QIAGEN). The integrity of RNA was detected by Agilent 2100 Bioanalyzer/Agilent 4200 Tape Station (Agilent Technologies), and the total amount and purity of RNA were determined by using Qubit 2.0 fluorescence quantitative instrument (Thermo Fisher Scientific) and NanoDrop ND-2000 spectrophotometer (Thermo Fisher Scientific). Similar to NGS library preparation, scRNA library preparation was also performed by DNA fragmentation, end repair, A-tailing, adaptor ligation and sample index PCR. The post ligation cleanup cDNA was amplified using the following program: 98 °C for 45s, followed by 15 cycles of 98 °C for 20 s, 67 °C for 30 s and 72 °C for 20s. 1 µl of sample (1:10 dilution) was added to an Agilent Bioanalyzer High Sensitivity chip(Agilent, 5067-4626) to determine the average fragment size. Library concentration was determined using the Qubit dsDNA HS Assay Kit(Thermo, Q32854)
GEO
GSM8972844_r1