Amplicons of V4-V5 16S rRNA genes from Ruddy Turnstone fecal samples
All samples were amplified and sequenced with the Archaea-Specific primers; DNA elutions were amplified using GoTaq Green Mastermix (Promega, Madison, WI, USA) and primers with index sequences for 2-step Illumina library preparation (Illumina, San Diego, CA, USA). Primer sequences are as follows with the index in italics and the target sequence in bold: Indexed516F (ACACTCTTTCCCTACACGACGCTCTTCCGATCTTGYCAGCCGCCGCGGTAAHACCVGC); Indexed915R (GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGTGCTCCCCCGCCAATTCCT). The PCR reaction included an initial incubation at 95C for 2 minutes, then 30 cycles of 95C for 60 seconds, 64C for 60 seconds, and 72C for 60 seconds and a final extension at 72C for 5 minutes. Amplification was confirmed using gel electrophoresis. Samples were sequenced on the Illumina MiSeq (Illumina, San Diego, CA, USA) platform at the University of Connecticut MARS core lab following library preparation.
SUB15094689
RUTU99