GSM9113791: CRISPRi_T0_rep2; Homo sapiens; OTHER
CRISPRi screen: genomic DNA was extracted from 1-2 e8 cells/sample as described by Gilbert et al., 2014; RNAseq: RNA was extracted from 4e5 cells/sample. All RIN were over 8. CRISPRi screen: Barcoded sgRNA were amplified and purified as described by Gilbert et al., 2014; RNAseq: cDNA libraries constructed from RNA. This step was outsourced to Novogene, and HISAT2 was used for mapping.
GEO
GSM9113791_r1