Total RNA-seq, strand specific, D.simulans(F) x D.mauritiana(M) S3
Total RNA was purified from 2-5 day old adult testes (100 pairs/sample) by TRIzol (Invitrogen) extraction according to the manufacturers instructions. Libraries were prepared for RNA sequencing using the KAPA Biosystems RNA HyperPrep Kit with RiboErase according to manufacturers directions with some modifications. This was followed by treatment with RNase H and DNase to remove rRNA duplexed to DNA and original DNA oligonucleotides. First-strand cDNA was generated using random primers.
SUB15199163
L459_1681