CRISPR Screening of Homo sapiens: 786-O cell line
Cells were again sorted and allowed to regrow to 3.0 x 107 cells. DNA was then isolated using a DNeasy Blood and Tissue Midi Kit (Qiagen) according to the manufacturers protocols. Guide RNA sequences were then amplified by PCR and purified using AMPure XP beads (Beckman Coulter) according to the manufacturers instructions.
SUB15411360
C1-001