Shot-gun metagenome of Halisarca dujardinii sponge, 2019 (Illumina)
DNA extraction was performed from 0.5 g of fresh H. dujardinii tissue using DNeasy PowerLyzer Microbial Kit (Qiagen, Hilden, Germany) according to the manufacturers instructions, including a bead-beating stage using a FastPrep-24 5G grinder (MP Bio, Santa Ana, CA, USA). A shotgun WGS library preparation was performed at Laboratory "Genomed" Ltd. (Moscow, Russia) using the MGIEasy Fast PCR-FREE FS Library Prep Set (MGI, China) according to the manufacturers protocol. Sequencing was performed using the DNBSEQ-G400 system (MGI, China) in a 150+150 nt paired-end mode.
SUB15321912
Hal