scTCR-seq of Homo sapiens - normal kidney tissue
Tumor and normal kidney samples were thawed after previously being cryopreserved. Dead cells were immunomagnetically depleted using the Dead Cell Removal Kit (Miltenyi), washed, and resuspended in PBS with 0.04% bovine serum albumin at a concentration of 1,000 cells per µL. Approximately 17,000 cells were loaded onto a 10X Genomics Chromium instrument (10x Genomics). Libraries were prepared using the Chromium single-cell V(D)J enrichment kit. Following library construction, quality control, and quantification, libraries were pooled and sequenced
NCI-phs003710
16097-111-NK-scTCR