FluvoAquicSoil_H2O2_2 (CRX1848564)
DNA extraction from soil samples using E.Z.N.A. Soil DNA Kit (Omega Bio-tek).  16S rRNA gene V3-V4 region (approx. 468 bp) amplified with primers 338F (5'-ACTCCTACGGGAGGCAGCAG-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3'). Purified PCR products used for library construction with NEXTFLEX Rapid DNA-Seq Kit (PerkinElmer).  Sequenced on Illumina NextSeq 2000 platform with 2x150 bp paired-end reads.
Institute of Soil Science, Chinese Academy of Sciences
DRX1001673
NGDC
CRX1848564