BS-seq of after 52 hours of 200 mM NaCl treatment in tetraploid soybean leaf rep3 (CRX1986222)
Genomic DNA was isolated by CTAB method, and sonicated into 100-1000bp using Bioruptor (Diagenode).Then the libraries were constructed through end-repair, A-tailing, adapter ligation and bisulfate-converted using a ZymoEZ DNA Methylation Kit.
NGDC
CRX1986222
Nanjing Agricultural University
DRX1007613