16S rRNA sequencing analysis of healthy people stool
The fermentation sample was centrifuged, followed by extracting microbial genomic DNA through use of a Stool Genomic DNA Extraction Kit (Solarbio, Beijing, China) following the manufacturer protocols. Primer pairs 338 F (5-ACTCCTACGGGAGGCAGCAG-3) and 806R (5-GGACTACHVGGGTWTCTAAT-3) were used to amplify hypervariable regions V3-V4 of 16S rRNA gene of bacteria using an ABI GeneAmp 9700 PCR thermocycler (ABI, CA, USA). Majorbio Biotechnology Co. Ltd. (Shanghai, China) was entrusted to perform 16S rRNA sequencing. Quality control was followed by grouping of selected sequences into operational taxonomic units (OTUs) through UPARSE (version 10.1 http://drive24.com/uparse/). OTUs were taxonomically annotated using Ribosomal Database Project (RDP)
SUB14886471
RSE-N11