Wildtype cytoplasmic dsRNA replicate 2
Strand-specific RNA sequencing library was prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina following manufacturers instructions. Briefly, the enriched RNAs were fragmented for 8 minutes at 94C. First strand and second strand cDNA were subsequently synthesized. The second strand of cDNA was marked by incorporating dUTP during the synthesis. cDNA fragments were adenylated at 3 ends, and indexed adapter was ligated to cDNA fragments. Limited cycle PCR was used for library enrichment. The sequencing library was validated on the Agilent TapeStation and quantified by using Qubit 4.0 Fluorometer as well as by quantitative PCR. Illumina 2x150bp paired-end sequencing was performed at a target depth of 50 million reads/sample.
SUB15923215
2