GSM8798346: Con 2; Homo sapiens; RNA-Seq
Total RNA from frozen specimen or cultured cells was extracted with the TRIzol reagent (Invitrogen, 15596026) according to the manufacturer's instructions. For ribosomal RNA-depleted (Ribo-) RNA-seq for two-paired breast cancer specimen and NT tissues, total RNA was depleted rRNA with the Illumina Rio-Zero Gold Kit (Illumina) and subjected to library preparation with the TruSeq Ribo Profile Library Prep Kit (Illumina) according to the manufacturer's instructions. For polyadenylated (poly(A)+) RNA-seq for MDA-231 cells with or without circRBM39 OE, total RNA was polyadenylated and subjected to library preparation.
GEO
GSM8798346_r1