RAD-Seq of Dugesia benazzii: adult specimen
Sequencing RADseq libraries were prepared following the procedures of Adapterama III (Bayona-Vsquez et al., 2019) with little modifications (Supplementary Material: Protocol), and DNA was digested using EcoRI-HF and XbaI as cutters. Concurrently, NheI-HF was used as blocker of adapter-dimer formations. After digestion, Illumina adapters were ligated onto the DNA fragments and individually barcoded samples were pooled. Indexed samples were subjected to PCR for 12 cycles of 98.0 C for 20 sec., 60 C for 15 sec., and 72 C for 30 sec., preceded by a 98 C step for 1 min, and finished with a 72 C step for 5 min. Library was size selected (between 400 and 600 bp) by running a 1% agarose gel, recovered using a QIAquick Gel Extraction Kit (28704, Qiagen)
SUB15157097
BU1391_01