EXJ201712 (CRX1731827)
High-quality genomic DNA was extracted from Tibetan antelope muscle tissue using QIAGEN kits and Binano. The genomic DNA was randomly fragmented using the Megaruptor nucleic acid shearing system. Large DNA fragments were enriched and purified using magnetic beads. Size selection of large fragments was performed via gel extraction using the BluePippin automated DNA size selection system. The fragmented DNA was subjected to damage repair. End repair and A-tailing were performed after purification. Adapters were ligated using the LSK108 kit following another round of purification. The library was quantified using Qubit. Upon completion of library preparation, the DNA library was loaded onto 4 Flow Cells and transferred to the PromethION sequencer for real-time single-molecule sequencing.
NGDC
CRX1731827
Yunnan University
DRX1000149