BCRseq of mus musculus: adult female Whole blood samples
RNA extraction testing was performed in the same way as for mRNA sequencing. About 2 g total RNA of each sample were used for BCR sequencing library preparation using KC-DigitalTM Stranded BCR-seq Library Prep Kit for Illumina 150 (Seqhealth Technology Co., Ltd., Wuhan, China, Cat. No. DT0815-02) following the manufacturers instruction. The library products corresponding to 250-500 bp were enriched, quantified and finally sequenced on NovaSeq (Illumina). The sequence was mapped to the international ImMunoGeneTics (IMGT) database. And using MiXCR software (version 3.0.3) to obtain V , D, and J fragments, rearrangement, and CDR3 sequences.
SUB14648547
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