GSM8552878: sgUSP22_input; Homo sapiens; ChIP-Seq
HepG2 cells were cross-linked with 1% formaldehyde for 10 minutes and quenched with 0.125 M glycine. Cells were then resuspended in SDS buffer. The resulting cell pellets were resuspended in ice-cold immunoprecipitation (IP) buffer, and subjected to sonication. Drosophila Schneider 2 cells (S2 cells) were prepared and processed in the same manner. Depending on the DNA concentration of each sample, chromatin from HepG2 cells was mixed with S2 cell chromatin at a ratio of 5:1. Subsequently, the chromatin was incubated overnight at 4°C with H2BK120ub antibodies. Protein A/G beads were then added at 4℃ for 4 hours. After thorough washes, samples were incubated in de-cross-linking buffer at 65°C for 6 hours, and DNA was extracted. The DNA fragments were repaired and dA-tailed, after which they were ligated to sequencing adaptors. The final DNA library was obtained by size selection and PCR amplification. Spike-in ChIP-seq
GEO
GSM8552878_r1