Illumina NovaSeq 6000 paired-end Sequencing of KAS20002693 (KAE21035540)
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Total RNAs were prepared from liver tissues of Agxt2-knockdown and control mice. The libraries were prepared for 150bp paired-end sequencing using TruSeq Stranded Total RNA Sample Prep Kit with Ribo-Zero H/M/R Illumina, CA, USA. Namely, mRNA molecules were purified and fragmented from 1microgram of total RNA using oligo dT magnetic beads. The fragmented mRNAs were synthesized as single-stranded cDNAs through random hexamer priming. By applying this as a template for second strand synthesis, double-stranded cDNA was prepared. After sequential process of end repair, A-tailing and adapter ligation, cDNA libraries were amplified with PCR Polymerase Chain Reaction. Quality of these cDNA libraries was evaluated with the Agilent 2100 BioAnalyzer Agilent, CA, USA. They were quantified with the KAPA library quantification kit Kapa Biosystems, MA, USA according to the manufacturer's library quantification protocol. Following cluster amplification of denatured templates, sequencing was progressed as paired-end 2x150bp using Illumina Novaseq6000 Illumina, CA, USA.
KOBIC
KAE21035540
Seoul National University College of Medicine
DRX602128