RNA-Seq of Ricinus communis: leaf
Total RNA was extracted from tissue samples. Concentration and purity were measured using a Nanodrop 2000, RNA integrity was assessed via agarose gel electrophoresis, and the RQN value was determined with the Agilent 5300. mRNA was enriched using Oligo(dT) magnetic beads that bind to polyA tails. Fragmentation buffer was added to randomly break the mRNA into ~300 bp fragments. cDNA was synthesized, adaptors were ligated, and the products were purified and size-selected. After PCR amplification, the final library was constructed and subjected to high-throughput sequencing.
SUB15449768
Con_1