Enzymatic Methyl-Seq of Ostrinia nubilalis: adult male abdomen
DNA isolated using Qiagen DNeasy kits. DNA was sheared using Covaris LE220 focused-ultrasonicator to 350 bp, then Enzymatic methyl-seq library preparation done using NEBNext Enzymatic Methyl-seq protocol (treated with TET2 and APOBEC). Samples were then barcoded individually, multiplexed, and sequenced on an Illumina HiSeq 4000 using 150 bp paired end sequencing.
SUB14656889
LLLM3