mRNAseq of C. elegans: dve-1
Biological replicates were grown on individual NGM plates seeded with E. coli (HT115) either expressing an empty L4440 vector (negative control) or dve-1 RNA for RNA interefence. RNA was extracted from 20 worms using using TRIzol LS Reagent (Thermo Scientific)  and Monarch Spin RNA Cleanup Kit (New England Biolabs, #T2040L). RNA integrity was examined on 2100 Bioanalyzer using the RNA 6000 Nano Kit (Agilent) and quantified using the RNA High Sensitivity Kit and Qubit 4.0 Fluorometer (Invitrogen). NGS libraries were prepared  with Illuminas TruSeq stranded mRNA LP Sample Prep Kit following Illuminas standard protocol (Part #15031047 Rev. E) but using  of the reagents according to the in-house  protocol of the Genomics CF (IMB Mainz).
SUB15722344
mRNAseq_dve1_rep4