GSM8972746: L4 C. elegans, vehicle control, rep3; Caenorhabditis elegans; RNA-Seq
Total RNA was isolated from animals using Trizol (Invitrogen) combined with Bead Beater lysis in five biological replicates for each genotype. An mRNA library (paired-end, 150-bp reads) was prepared for each sample/replicate using Illumina Truseq with PolyA selection (Azenta/GeneWiz).  Libraries were sequenced across two lanes on an Illumina HiSeq2500 (Azenta/GeneWiz), resulting in a range of 27-37M reads per sample, with peak mean phred scores of 38.76.
GEO
GSM8972746_r1