GSM8866010: Breast cancer PDEC, bexmarilimab, patient 131; Homo sapiens; RNA-Seq
Tissue pieces dissociated in 1 ml TRIsure (Bioline, cat. BIO-38032) using gentleMACS M tubes and by running program RNA.01_01 on gentleMACS Dissociator (both Miltenyi Biotec, cat. 130-093-235 and 130-093-236). Total RNA was extracted following Bioline's TRIsure protocol. mRNA purification with poly-T oligo-attached beads, reverse transcription using random hexamer primers, non-directional library preparation and sequencing were performed by Novogene (Cambridge, United Kingdom) using their standard protocols.
GEO
GSM8866010_r1