Long ONT reads used for the de novo assembly of Erwinia billingiae strain H255 genome
Total DNA was extracted from several suspended individual colonies of Erwinia billingiae strain H255 using Proteinase K/SDS treatment and Genomic DNA Clean & Concentrator-10 Kit (Zymo Research). Library preparation was then generally carried out in accordance with the ONT Rapid Barcoding Kit 24 V14 (SQK-RBK114.24) Nanopore-only Microbial Isolate Sequencing Solution (NO-MISS) protocol, using an individual barcode prior to pooling with other barcoded samples. The resulting library was sequenced using the FLO-MIN114 flow cell (R10.4.1) on a MinION Mk1C device. Basecalling was performed using the dna_r10.4.1_e8.2_400bps_sup@v5.0.0 model and was followed by demultiplexing. Demultiplexed reads were then filtered using Filtlong (v0.2.1; --keep_percent 90 --min_length 500 --min_window_q 20) prior to upload here.
SUB16240253
BMC_ONT_2026-01-08_Mk1C_SQK-RBK114-24_barcode17