GSM9178704: I46H8_LN_VV_D80_TCR; Mus musculus; OTHER
Lymphocytes were prepared by either digestion with liberase (submandibular gland) or mechanical dissociation (spleen) , followed by staining with fluorescent antibodies, hashing with Totalseq-C antibodies against MHCI and CD45,  and flow sorting CD3+ T cells into DMEM+ 2%FCS. Cells were stained using a custom Totalseq-C antibody panel then sorted again into DMEM + 2% FCS Libraries were prepared according to the 10x Genomics 5'v2 protocol for GEX, VDJ, and FBC
GEO
GSM9178704_r1