PacBio RNA-seq of Chrysanthemum chanetii:  control2 mature leaf
Total RNA was extracted from approximately 100 mg of frozen leaf tissue using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. For full-length transcriptome sequencing, 5 g of high-quality total RNA per sample was used to prepare Iso-Seq libraries. Three independent biological replicates were prepared for each treatment group and sequenced independently. cDNA synthesis was performed using the SMARTer PCR cDNA Synthesis Kit (Clontech, Mountain View, CA, USA) with specific primers, followed by PCR amplification to enrich full-length transcripts. Amplified cDNA was purified and used to construct SMRTbell libraries according to the PacBio Iso-Seq Express Template Preparation protocol. SMRTbell adapters were ligated to cDNA fragments, and DNA polymerase was added. Libraries were size-selected with BluePippin (Sage Science, Beverly, MA, USA) for fragments exceeding 1 kb.
SUB16268192
Pac-Cc-ct2