GSM9001839: CRC organoid from subject GBC007T; Homo sapiens; RNA-Seq
Total RNA was extracted from patient-derived tumor organoids using TRIzol reagent (Invitrogen) following the manufacturer's instructions. Organoids were harvested and lysed directly in TRIzol, followed by phase separation and RNA precipitation. The extracted RNA was washed, purified, and resuspended in RNase-free water. RNA integrity and concentration were assessed using a NanoDrop spectrophotometer and Agilent Bioanalyzer. mRNA was enriched from total RNA using poly(A) selection and fragmented. First- and second-strand cDNA synthesis was performed using the Illumina TruSeq Stranded mRNA Sample Preparation Kit. The resulting cDNA was end-repaired, A-tailed, ligated to sequencing adapters, and PCR-amplified to construct indexed libraries. Final libraries were quality checked and sequenced using the Illumina NovaSeq 6000 platform.
GEO
GSM9001839_r1