RNA-Seq of C. orientalis: eye
We euthanized the specimen using commercial Roxicaine anesthetic spray (lidocaine) and immediately dissected both eyes which were preserved in RNAlater (Invitrogen) for transcriptomic analysis. Tissue samples were sent to Macrogen Inc. (Seoul, South Korea) for RNA extraction, library preparation, and subsequent sequencing. Total RNA extracted from the eye tissues was processed for sequencing using the Illumina TruSeq Stranded mRNA LT Sample Preparation Kit, and the resulting libraries were then sequenced on the Illumina NovaSeq6000, yielding a sequencing depth of 100 million paired-end 100 bp reads.
SUB14827619
Corientalis_eye