GSM9083592: MGG8 cells, IDH1-mutant, vehicle treated_2; Homo sapiens; RNA-Seq
RNA was then extracted using the PureLink RNA Mini Kit (Invitrogen, 12183018A), followed by one-column PureLink DNase treatment. RNA yield and quality were assessed using a Nanodrop spectrophotometer (Fisher Scientific, AccuSkan FC) and stored at -80°C. 1.3 ug of total RNA was used for the construction of sequencing libraries. RNA samples were quantified using a Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA), and RNA integrity was checked using an Agilent TapeStation 4200 (Agilent Technologies, Palo Alto, CA, USA). ERCC RNA Spike-In Mix (ThermoFisher Scientific, 4456740), was added to normalized total RNA prior to library preparation following manufacturer's protocol. RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using the manufacturer's instructions (NEB, Ipswich, MA, USA).
GEO
GSM9083592_r1