Aerobic Granular Sludge (10 mg.L-1 of Phenol)
DNA was extracted from approximately 0.25 g of each granule sample using the DNeasy PowerSoil kit (QIAGEN, USA). Cellular lysis was performed with two runs in a FASTPREP-24 5G homogenizer (MP Biomedicals, USA) at 6.0 m/s for 40 seconds. A negative control was included in the extraction procedure. DNA quality was assessed by 0.8% agarose gel electrophoresis, and DNA concentration was measured using a Qubit 4 fluorometer (Thermo Fisher Scientific, USA). Extracted DNA was submitted for sequencing of the V4 region of the 16S rRNA gene by Novogene (www.novogene.com, USA). Sequencing was performed on an Illumina NovaSeq 6000 platform (Illumina, USA), generating 100,000 paired-end reads per sample, using the primers 515F (GTGCCAGCMGCCGCGGTAA) and 806R (GGACTACHVGGGTWTCTAAT)
SUB15992456
10mg