Amplicon of root vole: LD6
PCR amplification employs specific primers targeting the V3-V4 region of bacterial 16S rRNA. PCR products are quantified using the Quant-iT PicoGreen dsDNA Assay Kit on a Microplate reader (BioTek, FLx800), followed by pooling according to the required data volume per sample. Sequencing: The pooled library (10 nM) was diluted to an appropriate concentration and subjected to 2250 bp paired-end sequencing on an Illumina NovaSeq instrument using the NovaSeq 6000 SP Reagent Kit (505 cycles).
SUB15671371
LD6