GSM9083244: ENZ-con; Homo sapiens; RNA-Seq
Briefly, total RNA was extracted with Trizol (Invitrogen, Carlsbad, CA, USA), and subjected to mRNA isolation using GenSeq® mRNA Purification Kit (GenSeq, Inc., Shanghai, China). Then, the purified mRNA was used for library construction with GenSeq® Directional RNA Library Prep Kit (GenSeq, Inc., Shanghai,China) by following manufacturer's recommendations. The mRNA was fragmented into ~300 nt inlength. The first-strand cDNA was synthesized from the RNA fragments by reverse transcriptase and random hexamer primers, and the second-strand cDNA was synthesized in 2nd Strand Synthesis Buffer with dUTP Mix. Then, the double stranded cDNA fragments were subjected to end-repair and dA-tailing, followed by adapter ligation. The adapter-ligated DNA samples were PCR amplified andpurified to obtain sequencing libraries. Finally, the libraries were sequenced with sequencer on thepaired-end 150 bp mode.
GEO
GSM9083244_r1