ChIP-Seq for PARP1 MDA-MB-231 wt
ChIP-Seq: MDA-MB-231 wild-type cells were fixed with 1% formaldehyde for 10 min at RT. PARP1 was immunoprecipitated with anti-PARP1 Monoclonal Antibody (Cell Signaling Technology, Rabbit mAb, Cat# 9532, ID: 46D11) and dynabeads Protein G (ThermoFisher Scientific) as previously described (doi: 10.1016/j.biopha.2023.115798). Library for sequencing was prepared using the NEBNext Ultra DNA Library Prep Kit with Sample Purification Beads for Illumina. The analysis was performed using the Illumina NextSeq 500 System.
SUB15186767
P1MW