GSM9323533: LSK cells; Mus musculus; RNA-Seq
Bone marrow cells were isolated from the femora, tibiae, hips, and spines of sacrificed mice. Lineage-negative bone marrow cells were enriched using the following antibody cocktail: CD5, CD45R, CD11b, CD8a, Ly-6G, Ly-6C, and Ter119. FACS-based cell sorting was used to obtain 3984 total bone marrow cells, 3441 LK cells, and 1070 LSK cells (8495 cells in total). Library preparation was performed according to the manufacturer's instructions using the Single Cell 3' Reagent Kits v2 (10x Genomics) and the Chromium Controller (10x Genomics) to generate “Gel Bead-In-Emulsions” (GEMs). All libraries were sequenced using paired-end sequencing on the NovaSeq 6000 platform (Illumina).
GEO
GSM9323533_r1