GSM8833882: RNA from sample #2 from trial d, collected after 4 days of repeated exposure to treatment C (Tat-50ng); Homo sapiens; RNA-Seq
Astrocyte whole cell lysate was collected from the monocultures for protein analysis and RNA extraction. RNA extraction and purification was completed using the RNeasy Micro procedure (Qiagen, Germantown, MD, USA). Following the extraction protocol, RNA purity was assessed with a spectrophotometer (Nanodrop, ND-1000) to measure absorbance at 260 nm and 280 nm, quantity by Qubit fluorometry using the RNA HS Assay procedure, and quality using Agilent Bioanalyzer nano RNA chips. RNA samples with RNA integrity number (RIN) values ≥ 8.0 were selected for sequencing. Sequencing libraries were produced using the Illumina TruSeq Stranded mRNA library preparation with poly-A selection and sequenced on an Illumina NovaSeq SP chip at 2 x 100 nt paired-end reads with a target of >30 million clusters per sample.
GEO
GSM8833882_r1