Whole genome sequencing of bacterial isolate O-PF9-pnkrnd from Indian River Lagoon Pyrodinium bahamense lab isolate
Bacterial isolates were grown in marine broth at 25 C with shaking ~220 rpm. DNA was extracted from the bacterial isolates using the Qiagen Blood and Tissue Kit. Whole bacterial genome sequencing was performed by Novogene Genomics Service. Briefly, the genomic DNA of each sample was randomly sheared into short fragments of about 350 bp respectively. The obtained fragments were subjected to library construction using the NEBNext DNA Library Prep Kit followed by end repairing, dA-tailing, and further ligation with NEBNext adapter, the required fragments (in 300- 500 bp size) were PCR enriched by P5 and indexed P7 oligos. After purification and subsequent quality check, the library was ready for sequencing.
SUB14837971
O-PF9-pnkrnd_WGS