UCE target enrichment of Leptanilla zhg_my16, male (CASENT0842738)
DNA was extracted non-destructively using a DNeasy Blood & Tissue Kit (Qiagen Inc., Valencia, CA), with DNA eluted from spin columns using buffer AE at 56 degrees Celsius (Cruaud et al. 2019). Genomic concentrations were quantified for each sample with a Qubit 2.0 fluorometer (Life Technologies Inc., Carlsbad, CA). Phylogenomic data were obtained from these taxa using the hym-v2 probe set, with libraries being prepared and target loci enriched using the protocol of Branstetter et al. (2017). Enrichment success and size-adjusted DNA concentrations of pools were assessed using the SYBR FAST qPCR kit (Kapa Biosystems, Wilmington, MA) and all pools were combined into an equimolar final pool. The contents of this final pool were sequenced by an Illumina HiSeq X at Novogene Corporation Inc. Raw reads were assembled with SPAdes v. 3.12.0 (Bankevich et al. 2013). For trimming raw reads, use i7:GATCGGAAGAGCACACGTCTGAACTCCAGTCAC*ATCTCGTATGCCGTCTTCTGCTTG and i5:AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT*GTGTAGATCTCGGTGGTCGCCGTATCATT, where * indicates the index sequence.
SUB14804572
D3026