Stool sample of allo-HCT patient shotgun sequenced
After PicoGreen quantification and quality control by Agilent BioAnalyzer, 0.5ng-1.2g of genomic DNA was sheared using a LE220-plus Focused-ultrasonicator (Covaris catalog # 500569) and sequencing libraries were prepared using the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) with 8 cycles of PCR. Samples were sequenced PE100 on a NovaSeq 6000 with the NovaSeq 6000 S2 or S4 Reagent Kit (200 or 300 cycles). The average number of read pairs per sample was 32 million.
SUB15667504
FMT.0229J