GSM9113666: GSC4_KO404-1; Homo sapiens; RNA-Seq
Total RNA was extracted using RNAesay kit Qiagen. Total RNA was extracted by the user. Quantity and purity were assessed using Nanodrop. RNA integrity was checked on Bioanalyzer (Agilent). Samples below 25ng/μL were re-diluted. RIN >9 for most samples. Samples were diluted to 2.5 ng/μl, and 10 ng (4 μl) of each sample was randomly distributed on a 96-well plate. Barcodes were assigned and reverse transcription was performed. Samples were pooled and purified using the Zymo kit, followed by exonuclease treatment. Libraries were PCR-amplified (12 cycles), purified with magnetic beads, and double-stranded DNA was quantified. Tagmentation was performed using the Zymo kit, followed by a second PCR amplification (7 cycles) and magnetic bead purification. Final size selection of target fragments was done using magnetic beads.
GEO
GSM9113666_r1