Tannery Effluent
Extracted DNA from the samples were subjected to Nano Drop and GEL Check before being taken for PCR amplification: The Nano Drop readings of 260/280 at an ~ value of 1.8 to 2 is used to determine the DNAs qualityThe amplified 16s PCR Product is purified and subjected to GEL Check and Nanodrop QCThe Nano Drop readings of 260/280 at an ~ value of 1.8 to 2 is used to determine the DNAs qualityThe amplicons from each sample were purified with Ampurebeads to remove unused primers and an additional 8 cycles of PCR was performed using Illumina barcoded adapters to prepare the sequencing libraries. Libraries were purified using Ampurebeads and quantitated using Qubit dsDNA High Sensitivity assay kit. Sequencing was performed using Illumina Miseqwith 2x300PE v3-v4 sequencing kit.
SUB14891286
BRTTE