GSM8798783: Wild type, hemispheres ipsilateral to infarction; Mus musculus; RNA-Seq
After samples harvested, tissues were washed in ice-cold RPMI1640 and dissociated using Multi-tissue dissociation kit 2(Miltenyi Catalog No.130-110-204) from Miltenyi Biotec as instructions. DNase treatment was optional according to the viscosity of the homogenate. Cell count and viability was estimated using fluorescence Cell Analyzer (Countstar® Rigel S2) with AO/PI reagent after removal erythrocytes (Miltenyi Catalog No.130-094-183) and then debris and dead cells removal was decided to be performed or not (Miltenyi Catalog No.130-109-398/130-090-101). Finally fresh cells were washed twice in the RPMI1640 and then resuspended at 1×106 cells per ml in 1×PBS and 0.04% bovine serum albuminat. For single-cell RNA sequencing, the library was constructed following the SeekOne® MM Single Cell 3' Library Preparation Kit protocol. For single-nucleus RNA sequencing, the library preparation adhered to the protocol for the SeekOne® Digital Droplet Single Cell 3' Library Preparation Kit.
GEO
GSM8798783_r1