GSM8866041: Adjacent breast tissue, patient 99; Homo sapiens; RNA-Seq
Untreated, fresh tumor or adjacent tissue was cut into small pieces on ice using scalpel. Tissue pieces were digested in 0.2 mg/ml collagenase P (Sigma), 0.8 mg/ml dispase and 0.1 mg/ml DNase for 1h at +37°C. Cells were re-suspended in freshly-prepared 0,04% ultrapure BSA-PBS (ThermoFisher, cat. AM2616) for single-cell partitioning. Single-cell partitioning was done with Chromium Controller and scRNA-seq libraries were prepared using Chromium Single Cell 3' Library & Gel Bead Kit v2 and Chromium Chip A Single Cell Kit (10X Genomics, cat. PN-120237, cat. PN-120236) according to manufacturer's instructions. Full-length cDNA amplified by PCR (Veriti, Applied Biosystems) and cleaned using SPRIselect Reagent Kit (Beckman Coulter, cat. B23318). Amplified cDNA was fragmented, end-repaired and A-tailed prior to sample index PCR. Library quality and quantity were analyzed using Bioanalyzer (Agilent).
GEO
GSM8866041_r1