RNA-Seq of MRC-5 cell lines
Library preparation was performed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina, followed by paired-end sequencing (e.g., 2  150 bp) on an Illumina NovaSeq 6000 platform. Raw sequencing reads were quality-checked with FastQC, trimmed using Trimmomatic, and aligned to the human reference genome (GRCh38) via STAR aligner.
SUB15959121
B2