Short-read sequencing of mutation accumulation lines of Schizosaccharomyces pombe
We extracted genomic DNA from liquid cultures of 100 MA lines and 3 ancestral haploids using the Zymo YeaStar Genomic DNA Kit with the chloroform protocol (Zymo Research, Irvine, CA) and measured DNA concentration using the Qubit dsDNA HS Assay Kit (Life Technologies, Grand Island, NY). Sequencing libraries were prepared by the University of Wisconsin-Madison Biotechnology Center according to the QIAGEN FX DNA Library Preparation Kit (QIAGEN). Paired end, 150 bp sequencing was performed using the Illumina NovaSeq6000 (Illumina, San Diego, CA).
SUB15497732
23