GSM9801917: V3_ki67-myc+_ROI12_S1_Vehicle; Homo sapiens; OTHER
FFPE tumor sections were processed on the NanoString GeoMx Digital Spatial Profiler (DSP) at the Genomic Medicine Sweden Center North / Clinical Genomics Umea. Sections were stained with fluorescent antibodies against Ki67 and c-Myc. Within each ROI, cells were segmented into four populations: Ki67+MYC+, Ki67-MYC+, Ki67-MYC-, and Ki67+MYC-. UV-cleavable oligonucleotide tags from each segment (AOI) were collected for library preparation. Libraries were prepared using the NanoString GeoMx NGS Library Preparation Kit per manufacturer protocol. Human NGS Whole Transcriptome Atlas (WTA v1.0) panel (~18,000 genes). Each AOI received a unique indexing barcode. 92 AOIs across 10 tumors on four slides (S1-S4), each slide containing both conditions.
GEO
GSM9801917_r1