GSM9579997: Whole Embryo, 1 μM K02288, Rep1; Lytechinus variegatus; RNA-Seq
Embryos were lysed and total RNA was extracted using a Pure Link™ RNA Mini Kit (Invitrogen) following the manufacturer's protocol. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dTTP for non strand specific library or dUTP for strand specific library.  For the non strand specific library, it was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.  For the strand specific library, it was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina platforms, according to effective library concentration and data amount.
GEO
GSM9579997_r1