GSM9177520: #999_LTI05_d2_r2; Homo sapiens; RNA-Seq
Bulk RNA sequencing was performed on tissue from 4 separate PCLS. Briefly, PCLS were minced manually in 2 mm pieces in Trizol (ThermoFisherScientific) before bead homogenization for 95 seconds (40+20+15+10+10 seconds) at 50 Hz. RNA was purifiedusing a column-based workflow (Machery Nagel, 740984.250). RNA quantity and quality was assessed by RNA integrity number (RIN) via 4200 TapeStation system (Agilent). RNAseq libraries were prepared using Truseq stranded mRNA kit (Illumina; 20020594). Sequencing libraries were pooled and submitted for next-generation sequencing of 100bp single-end reads (Illumina NovaSeq sequencer, AGCT Core; Los Angeles, CA).
GEO
GSM9177520_r1