OF13500_2Soil
Approximately 1 g of qualified DNA per sample was fragmented to an average size of ~400 bp using a Covaris M220 ultrasonicator (Gene Company Limited, China). Paired-end libraries were constructed using NEXTFLEX Rapid DNA-Seq kits (Bioo Scientific, Austin, TX, USA). All 21 samples were individually barcoded and sequenced on an Illumina NovaSeq 6000 platform (NovaSeq S4 Reagent Kit v1.5, 2  150 bp) at Majorbio Bio-Pharm Technology Co., Ltd. (Shanghai, China), targeting approximately [6 Gb] of raw data per sample .
SUB16051048
OF13500_2