GSM9323650: OVCAR3 WT; Homo sapiens; RNA-Seq
Libraries for total RNA sequencing were prepared using the KAPA RNA HyperPrep Kit (Roche KAPA) according to the manufacturer's instructions. Briefly, 100 ng of total RNA was used as input, and ribosomal RNA (rRNA) was depleted using KAPA RiboErase HMR. The rRNA-depleted RNA was fragmented at 94 °C for 6 minutes using divalent cations. Fragmented RNA was reverse transcribed to synthesize first-strand cDNA. Second-strand synthesis with A-tailing generated double-stranded cDNA with dUTP incorporated into the second strand, enabling strand-specific sequencing. Indexed adapters with 3' dTMP overhangs were ligated to the fragments, followed by PCR enrichment (13 cycles). Libraries were purified and fragment size distribution was assessed using a Fragment Analyzer with the HS NGS Fragment Kit (Agilent).
GEO
GSM9323650_r1