GSM9834797: P301S mouse,contusions,48h,biological replicate1; Mus musculus; RNA-Seq
Spinal cord samples were homogenized in 500 µL of TRIzol reagent (Invitrogen, 15596018) using a grinder device and incubated at room temperature for 5 min. Homogenates were centrifuged at 15,800 × g for 10 min at 4 °C, and the supernatant was transferred to a new tube. Chloroform (200 µL) was added, and samples were vortexed and incubated for 3 min at room temperature. Following centrifugation at 15,800 × g for 15 min at 4 °C, 300 µL of the aqueous phase was collected and mixed with an equal volume of 70% ethanol. The mixture was then applied to an RNA extraction column (High Pure RNA Tissue Kit, Roche Diagnostics, 12033674001), and RNA was purified according to the manufacturer's instructions. Direct cDNA Library Prep Kit (SQK-NBD114.96; ONT)
GEO
GSM9834797_r1