GSM9324213: KrasG12D; PTEN-/-_2; Mus musculus; RNA-Seq
For RNA-seq:Total RNA was extracted from tissue samples using a standard column-based purification method following the manufacturer's instructions. RNA quality and integrity were assessed using the Agilent 2100 Bioanalyzer and only high-quality RNA was used for library construction. All RNA extractions were performed prior to submission to Novogene for RNA-seq library preparation and sequencing. For WGS:Genomic DNA was extracted from tumor tissues using a standard column-based DNA purification kit following the manufacturer's protocol. DNA purity and concentration were assessed using Nanodrop. High-quality genomic DNA was used for subsequent whole-genome sequencing (WGS), and the processed CNV data uploaded here were generated from the WGS results. RNA-seq libraries were prepared using Novogene's standard Illumina TruSeq-compatible mRNA-seq library construction workflow. Briefly, poly(A)+ mRNA was enriched using oligo(dT) magnetic beads, followed by fragmentation and synthesis of first- and second-strand cDNA. After end repair, A-tailing, and adaptor ligation, the cDNA products were PCR-amplified to generate sequencing-ready libraries. Whole-genome sequencing (WGS) libraries were prepared using a standard Illumina DNA library construction protocol. Genomic DNA was sheared to the desired fragment size, followed by end repair, A-tailing, adaptor ligation, and PCR amplification. Libraries passing quality control were sequenced on an Illumina platform, and the resulting WGS data were used for downstream CNV analysis.
GEO
GSM9324213_r1