DNA-Seq of Aspergillus niger DFA-N - Illumina
DNA was extracted from mycelium grown on Petri dish using the Qiagen DNeasy 96 PowerSoil Pro QIAcube HT Kit (#47021).Prior to extraction, samples were lysed using MagMAX Microbiome bead beating tubes. An additional lysis step was included: 20U of Zymo's Zymolyase Ultra was added and incubated at 37C for 1 hour prior to bead beating. DNA was was prepared for whole genome sequencing using the Illumina DNA Prep tagmentation kit (#20060059) with Illumina Unique Dual Indexes.Sequencing was performed on the Illumina NextSeq2000 platform using a 300 cycle flow cell kit to produce 2x150bp paired reads. 1-2% PhiX control was spiked into the run to support optimal base calling.Read demultiplexing, read trimming, and run analytics were performed using DRAGEN v4.2.7, an on-board analysis software on the NextSeq2000.
SUB15339620
SeqCoast_6119_Illumina