GSM9441714: OCI-AML3 StAMP Resistant BioRep1 Sample 1 Sequencing Batch 1; Homo sapiens; RNA-Seq
total RNA was isolated using the Qiagen RNeasy Mini Kit, following the manufacturer's protocol Libraries were generated from 200 ng of total RNA using the Roche Kapa mRNA HyperPrep strand-specific kit according to the manufacturer's guidelines, automated on a Beckman Coulter Biomek i7 Double-stranded DNA libraries were quantified using a Qubit fluorometer and Agilent TapeStation 4200. Dual-indexed libraries were pooled in equimolar amounts and initially shallow-sequenced on an Illumina MiSeq to assess library concentration and pooling accuracy. The final library pool was sequenced on an Illumina NovaSeq6000 with a target of 40 million 100 bp paired-end reads per sample
GEO
GSM9441714_r1