16S rRNA amplicon sequencing of Neltuma pallida: branch endophytic microbiome from asymptomatic trees
DNA was extracted from surface-sterilized branches of Neltuma pallida using the DNeasy Plant Pro Kit (Qiagen). The 16S rRNA gene was amplified and barcoded with the Oxford Nanopore 16S Barcoding Kit 124. Libraries were pooled and sequenced on a MinION Mk1C with R9.4.1 flow cells, producing full-length 16S rRNA reads.
SUB15580797
AA-01.2