hTERT-immortalized PDAC CAFs, BxPC3 co-cultured, mRNA, Pool B8
hTERT-immortalized patient-derived cancer-associated fibroblast were subjected to genetic perturbation with the Perturb-seq assay, and then co-cultured with BxPC3 tumor cell line. The cells were then loaded to 10X 5'HT Chip and run in Chromium X, followed by cDNA amplification and sequencing with S4 Illumina flow cell. Library construction was performed according to the 10X 5' CRISPR protocol.
SUB15425840
coculture_poolB8_GEX