GSM8766953: WT_Ly6Chigh_monocytes_1; Mus musculus; RNA-Seq
FACS sorted Ly6Chigh monocytes were sorted into Triziol LS. RNA was extracted using the Rneasy Micro Plus Kit (Qiagen) Total RNA was reverse transcribed into cDNA by selecting mRNA with oligo dT primer and SMART-Seq v4 oligonucleotides for template switching at the 5' end of the transcript. The double-strand cDNA was synthesized by LD-PCR amplification with the SMART-Seq® v4 Ultra® Low Input RNA Kit, followed by purification with AMPure XP beads and quantification with Qubit. cDNA was fragmented, end-repairment performed, A-tailed, and ligated with Illumina adaptors. After size selection and PCR enrichment, the library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection.
GEO
GSM8766953_r1