GSM9653143: Liver, 2months, sample6; Mus musculus; RNA-Seq
scRNA-seq libraries were prepared using the 10x Genomics Chromium platform with the Single Cell 3' Library & Gel Bead Kit v3.1 (1000121) and Single Cell G Chip Kit (1000120). Cell suspensions containing 300-600 viable cells/μL (quantified by Count Star viability analysis) were processed using the Chromium Controller to create gel bead-in-emulsion (GEM) partitions following the recommended workflow. Briefly, single cells were resuspended in phosphate-buffered saline (PBS) supplemented with 0.04% bovine serum albumin (BSA), with approximately 12,000 cells loaded per microfluidic channel, yielding an anticipated recovery rate of ~10,000 cells per sample. Cellular lysis and subsequent RNA barcoding were achieved through GEM-based reverse transcription. cDNA synthesis was conducted in a S1000TM Touch Thermal Cycler (Bio-Rad) using the following thermal profile: 45 min at 53 °C, 5 min at 85 °C, followed by 4 °C hold. Amplified cDNA products underwent quality control verification using the Agilent 4200 (service provided by CapitalBio Technology, Beijing). The constructed libraries were subsequently sequenced on an Illumina NovaSeq 6000 platform, employing a paired-end 150 bp (PE150) strategy, with a sequencing depth of at least 100,000 reads per cell. This sequencing process was carried out by CapitalBio Technology in Beijing.
GEO
GSM9653143_r1