Targeted Amplicon NGS-sequencing of Mus musculus : mouse zygote microinjected with synthetic Tpc1-I486, Tpc2-K188, Tpc2-L249, and Trpm4-L903 sgRNAs and ABE9-SpRY mRNA, replicate 3 , sequenced at the Tpc2-L249 locus , at stage E14
Samples were prepared for Amplicon-EZ-based NGS analysis (GeneWiz/Azenta Life Sciences). All locus-specific primers contained the 5 partial Illumina adapter sequences (Fwd: 5-ACACTCTTTCCCTACACGACGCTCTTCCGATCT-3, Rev: 5-GACTGGAGTTCAGACGTGTGCTCTTCCGATCT-3). Typically, samples corresponding to different target loci from each biological replicate were pooled into a single sample. The pooled NGS data were demultiplexed by mapping to the respective reference sequences later. Here, demultiplexed samples are provided via CRISPResso2 in CRISPRessoPooled mode.
SUB15465253
NGS_AC215