GSM8766796: GiMO_scRNA-seq_Day2; Homo sapiens; RNA-Seq
Cells were washed with cold D-PBS 3X in 30sec increments to dissolve Geltrex overlay. Next, samples were treated with 0.25% Trypsin/EDTA for 10 mins at 37 °C and 5% CO2.  After incubation, cells were quenched with cold E8 medium and centrifuged at 1.2 rpm for 3.5 min. Cells were then resuspended with FACS buffer, D-PBS containing 0.5% Bovine Serum Albumin (BSA, Sigma-Aldrich, # A3311), as a wash step and centrifuged at 0.7 rpm for 4 min. Cells were resuspended again with FACS buffer and dissociated to single cells using a Falcon 100 mm Cell Strainer (Corning, # 352360) and manually counted. hPSCs were then further dissociated using EASYstrainer Cell Sieves 40 mm filter and centrifuged at 0.3 rpm for 3 min and resuspended in FACS buffer at a total volume of 1000 cells mL-2. Library construction was performed using 10X Genomics Chrominium platform.
GEO
GSM8766796_r1