GSM9440158: WT (unedited control), Day 26 Fibrosa VICs; Homo sapiens; RNA-Seq
Gel-bead in emulsions (GEMs) were generated on the Chromium controller sample chip (10X Genomics) using live cell samples with an input of ~10,000 cells. Barcoded cDNA was then extracted from the GEMs by post-GEM RT-cleanup and amplified for 12 cycles. Amplified cDNA was fragmented and subjected to end-repair, poly A-tailing, adapter ligation, and 10X-specific sample indexing following the maufacturer's protocol. Libraries were quantified with Bioanalyzer (Agilent) and QuBit (ThermoFisher) analysis.
GEO
GSM9440158_r1