GSM9441967: E12.5 Mouse Placenta Control 1; Mus musculus; RNA-Seq
Total RNA was extracted from mouse placenta samples (with intact decidua layer) using a bead homogenizer and Trizol LS reagent (ThermoFisher, 10-296-028) /chloroform method. RNA concentration was measured using the NanoDrop 2000c spectrophotometer (Thermofisher Scientific). If necessary, an RNA clean and concentrator kit (Zymo Research R1019) was used to remove any contaminating DNA, phenol, and concentrate samples to improve 260/280 (1.9-2.0) and 260/230 ratios (2.0-2.2). Using the Illumina paired-end RNA-seq approach, we sequenced the transcriptome, generating a total of millon 2 x 150 bp paired-end reads.
GEO
GSM9441967_r1