GSM9179286: MEF_m6Aseq_input; Mus musculus; OTHER
RNAs were extracted using TRIzol reagent poly(A)-RNA was purified using Dynabeads mRNA DIRECT purification kit (Thermo Fisher), and was then immunoprecipitated against an m6A antibody (Synaptic Systems) bound to Protein G Dynabeads (Thermo Fisher), eluted by competition with free m6A (Sigma). The precipitated RNAs were reverse transcribed, amplified, tagmented and indexed using Smart-Seq2 protocol.
GEO
GSM9179286_r1