D12-2 (CRX799442; CRA012775)
Transcriptome library construction involves the purification and fragmentation of poly(A) RNA, followed by reverse transcription to generate cDNA, synthesis of U-labeled second-stranded DNAs, and modification of blunt ends with an A-base for adapter ligation. The adapter-ligated fragments undergo size selection, followed by treatment with heat-labile UDG enzyme and PCR amplification to create the final cDNA library with an average insert size of 300+/-60 bp.
China Agriculture University
DRX816815
NGDC
CRX799442