RNAseq of human breast cancer tumors: Sample Sample_PRE_RNA_186
Libraries were constructed from tumor RNA (up to 1 ug input) using the Illumina TruSeq RNA Sample Preparation Kit v2 (Catalog: RS-122-2001) and Agilent Bravo Automated Liquid Handler, with single, 6 bp index sequences (RS-122-9001DOC protocol). Library quantity was assessed using the Invitrogen Qubit dsDNA high-sensitivity assay (Catalog: Q32854) with the Thermo Scientific Qubit 3.0 Fluorometer (Catalog: Q33216), following the MAN0002326 protocol. Library quality was assessed using the DNA ScreenTape analysis (Catalog: 5067-5582) with the Agilent TapeStation 4200 instrument (Catalog: G2991AA), following the G2991-90031 protocol. Libraries were pooled in equal molar ratios and sequenced on Illumina HiSeq 2000/2500 sequencers using 2x75 bp pair-end reads to an average depth of 30 million clusters per library.
NCI-PHS003801
Sample_PRE_RNA_186