Ribo-seq of P. longispinus: whole-insects, polysomes
Whole insects were homogenized in ribosome-stabilizing buffer, and ribosome-associated RNA was isolated by sucrose gradient ultracentrifugation. Fractions corresponding to 70S ribosomes and polysomes were collected, RNA was extracted with TRIzol, depleted of rRNA, and prepared for Illumina sequencing to generate ribosome-associated transcript data
SUB16106845
PLONriboB