eDNA metabarcoding of marine sediment: COI sequencing
DNA was extracted from 10 g sediment of each sample by using the PowerMax Soil DNA Isolation Kit (QIAGEN, Germany) according to the manufacturers instructions. The primer set, mlCOIintF (5'-GGWACWGGWTGAACWGTWTAYCCYCC-3') and jgHCO2198 (5'-TAIACYTCIGGRTGICCRAARAAYCA-3'), targeting a fragment of the mitochondrial cytochrome c oxidase subunit I gene was used. PCR amplification was conducted in a volume of 20 L with three replicates. The high-throughput sequencing was performed on the Illumina MiSeq platform (320 bp paired-end) by Majorbio Co., Ltd. (Shanghai, China).
SUB15538667
CLC_C03_COI