DNA from a freshwater leech
Sample was stored in 90% ethanol. DNA was extracted using a Qiagen DNeasy kit. Preparation for sequencing of the 16S rRNA gene was carried out according to the Earth Microbiome Project protocol (Caporaso et al., 2011; Caporaso et al., 2012) with modifications as previously described (Case et al., 2015). Raw sequences were generated on an Illumina MiSeq platform at Laragen, Inc. (Los Angeles, CA, USA).
SUB16243660
Placobdella_SNWR_SC_37