ALG10
1)DNA samples,barcode,and common adapter pairs are plated and dried; 2)Samples are then digested with restrict enzyme;3)Adapters are ligated to the ends of genomic DNA fragments; 4)Pooling and purification;5)PCR: appropriate primers with binding sites on the ligated adapters are added and PCR is performed to increase the fragment pool; 6-7)Cleaned up PCR products, checked fragment sizes of the resulting library on a DNA analyzer.And sequence DNA
SUB15047418
ALG10_lib