GSM9066349: LC57; Homo sapiens; RNA-Seq
In brief, tissues were minced and enzymatically digested using a human tissue dissociation kit (Miltenyi Biotec Inc., Auburn, CA, USA, Cat# 130-095-929). The digested mixture was sequentially filtered and washed twice. Then, cells were cryopreserved in a solution of 10% DMSO and 90% fetal bovine serum at -80°C. For long-term storage, cells were placed in the vapor phase of a liquid nitrogen storage system. All gene expression libraries were sequenced on the Illumina NovaSeq 6000 system using paired-end reads with dual indexing: 28 cycles for Read 1, 10 cycles for i7 Index, 10 cycles for i5 Index, and 90 cycles for Read 2. A sequencing depth of approximately 20,000 read pairs per cell was targeted. In this study, we used only gene expression data.
GEO
GSM9066349_r1