GSM9441707: smallRNA_TC2_lin-28(xe192)_41h; Caenorhabditis elegans; ncRNA-Seq
At the time of sample collection, animals were washed off the plates with M9 buffer, washed twice with M9 buffer, resuspended in TRI Reagent (MRC, TR-118) and snap-frozen in liquid nitrogen. Prior to RNA extraction, animals were lysed by five cycles of thawing (42°C) and snap-freezing in liquid nitrogen. RNA was extracted using Direct-zol RNA Microprep kit (Zymo Research; R2062; DNase treatment included) according to the manufacturer's protocol. RNA was eluted in nuclease-free water (Thermo Fisher Scientific, Ambion, AM9937). Illumina TruSeq Small RNA Library Prep Kit
GEO
GSM9441707_r1