PacBio HiFi sequencing data for Convolvulus arvensis
High molecular weight (HMW) genomic DNA (gDNA) was extracted from 400 mg of young leaves following the cetyltrimethylammonium bromide (CTAB) method as described in Inglis et al., 2018, with the following modifications: (i) in the sorbitol wash buffer and CTAB solution, we used sodium metabisulfite (1% w/v) instead of 2-mercaptoethanol (1% v/v); (ii) we repeated the tissue homogenate wash steps until the supernatant turned clear; (iii) we performed the CTAB lysis step at 45 C and (iv) the chloroform extraction step twice using ice-cold chloroform.  The DNA purity (260/280 = 1.79 and 260/230 = 2.35) was assessed by absorbance ratios measured using the NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific, MA). The DNA yield (101 g) was quantified using QuantiFluor ONE dsDNA Dye assay (Promega, WI). The size distribution of the DNA was estimated using the Femto Pulse system (Genomic DNA 165 kb kit, Agilent, CA), and found that 57 % of the total fragments were 30 kb or longer.
SUB15469097
CCGP_gDNA0124