GSM9769786: hMPV1; Homo sapiens; RNA-Seq
A549 cells were infected with hMPV, MuV, VSV, SeV or mock-infected with DMEM (n=3). The RNA-seq transcriptome library was prepared following Illumina® Stranded mRNA Prep Ligation from Illumina (San Diego, CA) using 1μg of total RNA. Briefly, messenger RNA was isolated according to the polyA selection method by oligo(dT) beads and then fragmented by the fragmentation buffer firstly. Secondly double-stranded cDNA was synthesized using a SuperScript double-stranded cDNA synthesis kit (Invitrogen, CA) with random hexamer primers (Illumina). Then the synthesized cDNA was subjected to end-repair, phosphorylation and 'A' base addition according to Illumina's library construction protocol. Libraries were size-selected for cDNA target fragments of 300 bp on 2% Low Range Ultra Agarose followed by PCR amplification using Phusion DNA polymerase (NEB) for 15 PCR cycles. After quantification by Qubit 4.0, the paired-end RNA-seq sequencing library was sequenced with the NovaSeq X plus sequencer (2 × 150 bp read length).
GEO
GSM9769786_r1