GSM8899514: RNA-seq_WT-R1; Homo sapiens; RNA-Seq
Approximately 2.5×10e6 cells were used as a standardized experimental unit to ensure reproducibility for COCA-seq assay. O-GlcNAz chromatin DNAs enriched by Streptavidin-magnetic beads were purified with the MiniBEST DNA Fragment Purification Kit Ver.4.0 (Takara) and the MiniBEST Agarose Gel DNA Extraction Kit Ver.4.0 (Takara). For RNA-seq, total RNA was extracted from the MCF-7 and MCF-7/DOX cells using Trizol reagent (Invitrogen). RNA quality was checked. DNA-end repair, 3'-dA overhang and ligation of methylated sequencing adaptor. PCR amplification and size selection. Qualified library for sequencing. RNA-seq libraries were constructed using SMARTer cDNA library construction kit.
GEO
GSM8899514_r1