WGS of RPE-1 cells, UHMW, UL, PromethION 24
UHMW DNA from 6 x 106 cells was used to generate UL ONT libraries with the UL DNA sequencing kit (ONT SQK-ULK114) following manufacturers instructions. Ninety l of library was loaded in a R10.4.1 (FLO-PRO114M) flow cell and sequenced on the PromethION 24, with two nucleases washes and reloads after 24 and 48 hours of sequencing. Basecalling was performed using Guppy v6.3.9 in super-accuracy mode.
SUB15274463
RPE1_ULK114_20221215