GSM8518357: M01_Blood_CM_Nr4a1KO_MDP_2; Mus musculus; RNA-Seq
For I-NCM and N-NCM: PBS or MDP was administered to mice by I.V. injection at a dose of 10-20 mg/kg mouse body weight. At 36h post treatment, the blood samples were collected from submandibular (facial) vein or cardiac puncture.  For PD-L1+ and PD-L1- NCMs:  the blood samples were collected from wtB6 mice and the NCMs were isolated by Fluorescence-activated Cell Sorting (FACS). Total RNA was extracted from mouse blood monocytes using an Arcturus PicoPure RNA Isolation Kit (Cat#: KIT0204, Thermo Fisher Scientific) or RNeasy Plus Micro Kit (Cat#: 74034, Qiagen). Qualified total RNA samples were preamplified, and cDNA synthesis was performed using the SMART-Seq v4 Ultra Low Input RNA Kit for sequencing (Clontech/Takara Bio, USA).
GEO
GSM8518357_r1