GSM8899542: Healthy Control3; Homo sapiens; RNA-Seq
RNAs were isolated from exosomes using the MagMAX™ mirVana™ Total RNA Isolation Kit (Thermo Scientific), following the manufacturer's protocol. After isolation, the RNA concentration was determined using a NanoDrop spectrophotometer (Thermo Scientific), and RNA integrity was assessed with an Agilent Bioanalyzer (Agilent Technologies). For library preparation, the isolated RNA was processed using the NEXTFLEX® Small RNA-Seq Kit v4 with UDIs (Revvity) according to the manufacturer's instructions. This protocol involved 3' and 5' adapter ligation, reverse transcription, PCR amplification, and size selection. Libraries were quantified using Qubit dsDNA HS Assay (Thermo Scientific) and validated for size distribution using a Bioanalyzer (Agilent Technologies). Sequencing was performed on an Illumina NovaSeq X Plus platform, following the standard procedures for small RNA sequencing.
GEO
GSM8899542_r1