GSM9144595: E3_47APOT1A; Mus musculus; OTHER
Cells were washed with PBS, treated with Trypsin/EDTA, and neutralized with growth media. Genomic DNA was isolated, digested, and telomere sequences were enriched following the same protocol described for the dot blot assay. A total of 100-150 ng of enriched telomeric DNA was then sonicated using a Covaris S220 Ultrasonicator to generate DNA fragments ranging from 300-500 bp. Library preparation for Illumina sequencing was performed using the NEBNext Ultra II kit, following the manufacturer's instructions.
GEO
GSM9144595_r1