RNA-seq of mouse laser captured layer 1
All layer 1 and layers 2-6 samples were pooled together and processed for RNA extraction, which wasdone using Absolutely RNA Nano Kit (Agilent Technologies; #400753) and included a DNAse I step.Total RNA was eluted in 15 _L RNase free water and its quality was assessed using RNA 6000 Pico Kit(Agilent Technologies, #5067-1513) on a Bioanalyzer (Agilent Technologies). Libraries were preparedfrom total RNA ranging from 1.2 to 6.6 ng using NEBNext Ultra II Directional RNA Library Prep Kit forIllumina (#E7760S) and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs,#E6440). mRNAs were enriched using the NEBNext poly(A) mRNA Magnetic Isolation Module (#E7490).Final cDNA libraries were assessed using Agilent High Sensitivity DNA assay (Agilent Technologies,#5067-4626) and sequenced on Illumina NextSeq2000, using a paired-end 75-bp sequencing run.
SUB15792206
lasercapture_L1_biorep4