GSM9066835: diff SC-EV lncRNA biol rep 1; Rattus norvegicus; RNA-Seq
1. Whole-transcriptome RNA sequencing was performed by the Genomics, Epigenomics, and Sequencing Core (GESC) at the University of Cincinnati following updated protocols. Total RNA was isolated from Schwann cells for mRNA-seq and SC-EVs for lncRNA-seq, and quality control was assessed using the Agilent Bioanalyzer with the RNA 6000 Pico Kit. 2. For MicroRNA-Sequencing, total RNA was extracted from SC-EVs using the miRNeasy Micro kit as per the manufacturer's suggested protocol and was quality control-assessed using the RNA 6000 Pico Kit on the Agilent Bioanalyzer 1. For whole RNA sequencing for mRNA and lncRNA, 10 ng of total RNA was used as input for library preparation using the NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs, Ipswich, MA).  2. For microRNA-Sequencing, ~5 ng of total RNA was used for library construction with the NEBNext Multiplex Small RNA Library Prep Kit (New England BioLabs, Ipswich, MA), incorporating a protocol modification to increase sensitivity and specificity for small RNA detection.
GEO
GSM9066835_r1