GSM9066803: Dermal fibroblast microtissue, TGFb- and Q-peptide-treated; Homo sapiens; RNA-Seq
Total RNA was extracted with Qiazol. Tissues were lyzed with Qiazol, chloroform was added for phase separation, isopropanol was used to precipitate RNA from the aqueous phase, and samples were washed twice with 75% ethanol before being resuspended in 12 µL of Ultrapure RNase-free water. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. RNA strands were fragmented, then the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis. This was followed by A-tailing, adapter ligation, size selection, amplification, and purification, to produce the library, which was checked using Qubit and real-time PCR for quantification, and bioanalyzer for size distribution detection. The libraries were then pooled and put through Illumina sequencing.
GEO
GSM9066803_r1