ITS amplicon sequencing of Vitis amurensis 'Beibinghong': spontaneously fermenting must at day 0
Fungi ITS regions were amplified using primer pairs ITS1F (5-CTTGGTCATTTAGAGGAAGTAA-3) and ITS2R (5-GCTGCGTTCTTCATCGATGC-3) on a PCR system (GeneAmp 9700, Applied Biosystems, USA). The PCR system consisted of 10 L of 2  Pro Taq, 0.8 L of Forward Primer (5 M) and Reverse Primer (5 M), Template DNA (10 ng/L), and ddH2O supplementation to 20 L. PCR cycling conditions were as follows: 95 C for 3 min, and PCR was run for 35 cycles of 30 s at 95C, 30 s at 53C, and 45 s at 72C, followed by a final extension step for 10 min at 73C.
SUB15429477
B0_b