GSM9834125: WTC11 hiPSC, Passage 63, Day 113 hPSC-CMs, Well Replicate 4; Homo sapiens; RNA-Seq
RNA was harvested from Trizol-collected samples by tri-phasic extraction followed by purification and concentration with Zymo Direct-zol MiniPrep Plus columns (including on-column DNAase treatment). 500ng of total RNA per sample was used for the construction of sequencing libraries. Poly(A)-selected mRNA-seq libraries were prepared by Novogene from total RNA using poly-T oligo-attached magnetic beads, followed by RNA fragmentation, random-hexamer-primed cDNA synthesis, adapter ligation, size selection, PCR amplification, and purification. Libraries were sequenced on an Illumina NovaSeq 6000 platform using paired-end 150-bp reads.
GEO
GSM9834125_r1