lignite_V3V4
DNA was isolated by extracting cells from lignite sample, followed by in-house, PCI based DNA purificaition protocol. Isolated DNA was subjected to amplification of V3V4 hypervariable region of 16S rRNA gene using 341F and 785R primers. Library preparation was conducted using standard Illuma protocol. Sequecing was performed on Illumina MiSeq platform with 2x250 paired ends approach.
SUB14889088
lignite_3