WT_2 (CRX1908335)
The RNA-seq transcriptome library was prepared using 10 ng of total RNA with the SMART-Seq V4 Ultra Low Input RNA Kit for Sequencing (Clontech, USA). Reverse transcription (one-strand synthesis) was firstly performed, and RNA with a polyA tail (major mRNA) was reverse transcribed using an Oligo(dT) primer. During reverse transcription, three cytosines (C) were added to the cDNA 3' end due to using MMLV RT. Subsequently, TSO primer synthesized double-stranded cDNA. The cDNA was PCR-amplified to nanogram levels, then fragmented by modified Tn7 transposase with linker addition.
NGDC
CRX1908335
Yangzhou University
DRX1002874