WGS of RPE-1 cells, HMW, UL, PromethION 2 Solo
HMW DNA from 6 x 106 cells was used to generate UL ONT libraries with the UL DNA sequencing kit (ONT SQK-ULK114) following manufacturers instructions. Ninety l of library was loaded in a R10.4.1 (FLO-PRO114M) flow cell and sequenced on the PromethION 2 Solo, with two nucleases washes and reloads after 24 and 48 hours of sequencing. Basecalling was performed using Guppy v6.5.7 in high-accuracy mode.
SUB15274463
RPE1_ULK114_20230728