GSM9179607: Tasor_1; Mus musculus; OTHER
Briefly, 1 × 10^5 cells were treated with 10 μL concanavalin A-coated magnetic beads, followed by incubation with primary antibodies. After removal of primary antibody using magnet stand, Goat Anti-Rabbit IgG H&L was diluted at 1:100 in dig wash buffer, and cells were incubated at RT for 1 hr. Then, pA-Tn5 adapter complex was added and incubated with samples at RT for 1 hr. Next, genomic DNA was purified using Tagment DNA Extract Beads. The libraries were constructed by mixing 21μL DNA with 2μL of a universal i5 and a uniquely barcoded i7 primer.
GEO
GSM9179607_r1