PacBio HiFi seuqnece of Equus Kiang
For the construction of the PacBio library, we used the genomic DNA of E.kiang, sheared it to ~20-kb fragments, and those smaller than 7 kb were filtered out using BluePippin (Sage Science, MA, USA). The filtered DNA was then converted into the proprietary SMRTbell library using the PacBio DNA Template Preparation Kit following the manufacturers instructions. Single Molecule Real Time (SMRT) sequencing was conducted on a PacBio Sequel II sequencing platform using HiFi Bundle (v2) sequencing reagent and the SMRT Cell (8M).
SUB11617156
PacBio_HiFi