GSM8260938: LPS_500_2.R1,Bone marrow derived macrophages, 200ng/ml LPS 24h; Mus musculus; RNA-Seq
The preparation of RNA samples is the first step of transcriptome sequencing. For LPS-induced BMDMs, RNA within the cells is extracted and purified through a sequence of steps including lysis with Trizol, phase separation with chloroform, precipitation with isopropanol, and washing with 75% anhydrous ethanol. Total RNA was extracted from mouse BMDMs and primary chondrocytes using TRIzol reagent (NR0002, Leagene, China). Chloroform and isopropanol were subsequently added, followed by centrifugation to precipitate the RNA at the bottom. After that, the precipitated RNA was washed with 75% anhydrous ethanol, and upon drying, it was resuspended in RNase-free water to complete the RNA extraction process. For mRNA quantification, reverse transcription was carried out using 5x HiScript II qRT SuperMix II (Vazyme Biotech, Nanjing, China). Each PCR amplification reaction consisted of 10μl 2X ChamQ SYBR qPCR Master Mix (Vazyme), 10μM of both forward and reverse primers, and 500 ng of cDNA.
GEO
GSM8260938_r1