WT_Stim2 (CRX2175836)
RNA was isolated using TRIzol reagent (Invitrogen) according to the manufacturer's instructions. RNA purity and quantification were evaluated with a NanoDrop 2000 spectrophotometer (Thermo Scientific and RNA integrity was evaluated with the Agilent 2100 Bioanalyzer (Agilent Technologies). Then libraries were constructed using the VAHTS Universal V5 RNA-seq Library Prep Kit (Vazyme), following the manufacturer's instructions. The libraries were sequenced on the Illumina NovaSeq 6000 System, and 153 bp paired-end reads were generated.
NGDC
CRX2175836
Shanghai Jiaotong University School of Medicine
DRX1010235