eDNA metabarcoding of marine sediment: 28S sequencing
DNA was extracted from 10 g sediment of each sample by using the PowerMax Soil DNA Isolation Kit (QIAGEN, Germany) according to the manufacturers instructions. The primer set, 1096F (5'-CTYAACCTATTCTCAAACT-3') and 1535R (5'-TATTTGCTACTACCACCAAG-3'), targeting a fragment of the 28S rRNA gene was used. PCR amplification was conducted in a volume of 20 L with three replicates. The high-throughput sequencing was performed on the Illumina MiSeq platform (300 bp paired-end) by Majorbio Co., Ltd. (Shanghai, China).
SUB15538667
SJC_C01_28S