16S Metagenomics: bacterial species identification from Vitis vinifera samples
The NGS sequencing project started with quality control of the DNA samples, where the DNA was analysed to ensure samples had sufficient integrity (1,5% Agarose gel electrophoresis) and quantity (Qubit) for optimal amplification;  The library construction was performed using the Illumina 16S Metagenomic Sequencing Library preparation protocol with a specific set of primers: 341F (CCTACGGGNGGCWGCAG) and 785R (GACTACHVGGGTATCTAATCC);  The generated DNA fragments (DNA libraries) were sequenced with MiSeq Reagent Kit v3 in the lllumina MiSeq platform, using 300bp paired-end sequencing reads.
SUB14896648
N2919-16S_Lib