GSM9324310: BMC Murine Plateau Arthritis Clo-lip Synovial LiveCD45+CD11b+Ly6g-SiglecF-CD64-MHCII-Ly6c-CD177- cells, CITE-seq; Mus musculus; OTHER
Cellular Indexing of Transcriptomes and Epitopes by Sequencing was performed on isolated CD45+CD11b+Ly6G-SiglecF-CD64-MHCII-Ly6c-CD177- cells from the joint synovium from baseline (day 0), peak (Day 7), plateau (Day 14), and resolution (day 56) stages of STIA. Generation of GEMs, barcoding, cDNA amplification, 3′ gene libraries, and cell surface protein libraries was conducted using the Chromium Next GEM Single Cell 3′ (v3.1, dual index) protocol with Feature Barcode technology. Sequencing was performed on a NextSeq 2000 (Illumina) at the same facility.
GEO
GSM9324310_r1