GSM8898970: Young_Male; Mus musculus; RNA-Seq
Visceral adipose tissue (VAT) was digested in 0.8mg/mL collagenase II (Worthington Biochemicals), 3% BSA (Sigma), 1.2mM Calcium Chloride, 1.0mM Magnesium Chloride, 0.8mM Zinc Chloride, and 1x HBSS (Life Technologies 14185-052) for 40-45 minutes in a 37°C water bath with vigorous shaking. The Stromal Vascular Fraction (SVF) was filtered on a 100um cell strainer. Red blood cell lysis was performed using ACK lysis buffer, and cells were filtered a second time with a 70um cell strainer. Following adipose tissue digestion, stromal vascular fraction (SVF) cells isolated from VAT were then stained with viability dye, CD45.2, CD45, CD3, CD19, SiglecF, F4/80, CD11b, and were were sorted using a BD FACSAria II. Library was generated according to manufacturer's instructions (Chromium Next GEM Automated Single Cell 3' cDNA Kit v3, 10X Genomics)
GEO
GSM8898970_r1