GSM8865490: siCont,rep1; Mus musculus; RNA-Seq
Ascites samples were collected in 50-ml conical tubes (BD), followed by centrifugation for 10 min at 400g at 4degree. The supernatant was aspirated and the remaining pellet was resuspended with 5 mL of ACK lysis buffer (Life technologies) and incubated for 5 min. 30 ml of PBS was used to quench the lysis buffer followed by centrifugation for 10 min at 400g at 4degree. The concentration of single-cell suspensions was adjusted to about 500–1,200 cells per μl. Library was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics)
GEO
GSM8865490_r1