GSM8607971: syy-3; Mus musculus; RNA-Seq
Total RNA was extracted using TRIzol reagent (9109, TaKaRa, Kusatsu, Shiga, Japan) and reverse transcribed into cDNA using a cDNA synthesis kit (RR047A, TaKaRa) according to the manufacturer's instructions. RNA purity and quantification were assessed using a NanoDrop 2000 Spectrophotometer (Thermo Scientific, USA), while RNA integrity was evaluated with an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The transcriptome library was constructed following the manufacturer's instructions using the VAHTS Universal V5 RNA-seq Library Prep Kit.
GEO
GSM8607971_r1