GSM9473909: H1-hESC-CM, Clipper GapmeR Knockdown, Replicate 5; Homo sapiens; RNA-Seq
Directed differentiation into cardiomyocytes were performed. Beating hESC-CMs were transfected 9 days after the initiation of differentiation with 50 nM of either Control or CLIPPER-GapmeRs using Lipofectamine™ RNAiMAX Transfection Reagent (Thermo Fisher #13778150), and harvested 48 hours later for transcriptomic analysis. Total RNA was extracted using Trizol Reagent (15596026, ThermoFisher) and Direct-Zol RNA miniprep Kit (Zymo Research, R2050) according to manufacturer's protocol. RNA quality and yield was accessed using Agilent RNA 6000 pico kit (50167-1513, Agilent) for quality. RNA quality and yield was accessed using Agilent RNA 6000 pico kit (50167-1513, Agilent) for quality. Bulk RNA-seq library preparations were prepared using TruSeq Stranded Total RNA library Prep HMR kit (20020596, Illumina). cDNA libraries were quantified using the Agilent DNA1000 kit (5067-1505, Agilent), prior to sequencing on Illumina NextSeq Sequencing platform by Macrogen Asia Pacific Pte. Ltd.
GEO
GSM9473909_r1