GSM9114643: siWTAP3; Homo sapiens; RNA-Seq
Sample purity detected by Nanof Hortomel ® spectrophotometer (Thermofischer Semerfiche); Agilent 2100 RNA Nano 6000 Assay Kit (Agilent Technologies, CA, USA) was used to test RNA samples Integrity and concentration of the product. The 3' end of eukaryotic mRNA had a polyA tail structure, and magnetic beads with Oligo(dT) were used for enrichment and purification. Fragmentation Buffer is added to the purified mRNA to make the fragments short. Then, the fragmented RNA was used as a template, and the first strand of cDNA was synthesized by random primers for reverse transcription, and the second strand of cDNA was synthesized by adding 2nd Strand Marking Buffer and 2nd Strand/End Repair Enzyme Mix. After end repair, base A was added, sequencing adapter was added, the target fragment was recovered by magnetic bead screening, and PCR amplification was carried out to complete the entire library preparation.
GEO
GSM9114643_r1