RNA-seq of WT_1 (CRX1925182; CRA029190)
mRNA was enriched from total RNA using oligo(dT) magnetic beads. Following RNA fragmentation, the first strand of cDNA was synthesized using random hexamer primers, followed by synthesis of the second strand. Subsequently, the cDNA underwent end repair, A-tailing, adapter ligation, fragment selection, amplification, and purification to construct the final sequencing library. After quality assessment, the qualified libraries were quantified, pooled according to the concentration of valid libraries and the required sequencing data volume, and then sequenced on the Illumina platform.
NGDC
CRX1925182
Southwest University of Science and Technology
DRX812083