16S rRNA V4 amplicon sequencing of wood frog: tadpole gut
Tadpole intestinal tracts were dissected with forceps sterilized in 50% bleach and DNA was extracted using Qiagen DNeasy PowerSoil Pro Kits. The 16S rRNA V4 region was PCR-amplified using New England Biolabs reagents and dual-index barcoding, purified with the GeneJet PCR Purification kit, quantified on a Qubit fluorometer, and sequenced on an Illumina MiSeq at the Yale Center for Genome Analysis.
SUB15197080
103_S230