GSM8834202: RBC-step2-1F-r1; Homo sapiens; ATAC-seq
20,000 to 50,000 cells were washed in cold PBS and resuspended in 50ul Lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% Igepal) and immediately spun at 500 xg for 10 min at 4 C. The supernatant was then discarded and the nuclei resuspended in the transposase reaction mix (25 ul 2x TD Buffer, 2.5 ul Transposase (Illumina), 22,5 ul nuclease-free water) and incubated for 30 min at 37C. Immediately after, DNA is purified using a MinElute Kit (Qiagen). Tagmented DNA was PCR amplified to amend sequencing barcodes and qPCR was used to estimate the number of PCR cycles for exponential amplification. Libraries were pooled and sequenced at 150bp paired-end at Azenta.
GEO
GSM8834202_r1