GSM9579629: day 30, Mock - MuHV4 - del73, mRNA, AbSeq; Mus musculus; OTHER
Whole bone marrow (BM) cells were incubated for 20 min with a purified rat IgG2a anti-mouse CD16/CD32 antibody (BioLegend) to block Fc receptor-mediated nonspecific binding. Cells were then stained for 30 min at 4°C with APC-conjugated antibodies against lineage markers (CD3, B220, Gr-1, Ter119, NK1.1, SiglecF, and CD11b). The cell suspension was subsequently incubated with anti-APC magnetic beads (BioLegend) for 15 min. Cells were passed through LS columns, and the negative (flow-through) fraction was collected and used for subsequent sorting of LSK cells. For bone marrow monocytes, T cells, CMP/GMP populations, and blood monocytes, cells were incubated with an Fc receptor–blocking antibody followed by staining with fluorochrome-conjugated antibodies against surface markers. Cell sorting was performed using a BD FACSAria™ III cell sorter. Gating strategies are shown in the manuscript. Libraries  for single-cell immune profiling were constructed following ™ HT Single-Cell Analysis System protocols from BD rhapsody manufacturer.
GEO
GSM9579629_r1