GSM9145638: EV71_3; Enterovirus A71; RNA-Seq
Total RNA was extracted using TRIzol, on-column DNase digestion to eliminate genomic DNA contamination. The extracted RNA samples were sent to Jingneng Biotechnology (Shanghai, China) for subsequent transcriptome sequencing. RNA extraction and preparation: Total RNA is extracted from the sample, and mRNA is enriched (typically through polyA tail selection), followed by fragmentation. cDNA synthesis and library construction: The fragmented mRNA is reverse transcribed into double-stranded cDNA, sequencing adapter linkers are ligated to both ends, and fragment screening and library quality control are performed. The final product is a cDNA library suitable for high-throughput sequencing.
GEO
GSM9145638_r1