DNA-seq of Spinibarbus caldwelli:Muscle
For PacBio sequencing, the qualified high-quality genomic DNA (gDNA) was sheared to a size range of 1520 Kp. Subsequently, a SMART bell library was constructed following the manufacturers protocols. The library was then sequenced on the PacBio Sequel II system using the CCS (Circular Consensus Sequencing) mode. After filtering out polymerase reads, raw subreads were processed with SMARTLink (version 8.0) using the parameters min-passes=3 min-rq=0.99 to generate HiFi reads. This process yielded a total of 1.76 Gb of HiFi reads with an average read length of 18.33 Kb.
SUB14808441
sample2