16S rRNA gene sequencing of mouse fecal microbiome
16S rRNA gene sequencing was performed using the Illumina MiSeq platform with a modified version of the bTEFAP method, which amplifies the V4 region of the 16S rRNA gene. PCR primers 515F (GTGYCAGCMGCCGCGGTAA) and 806R (GGACTACNVGGGTWTCTAAT) were used for amplification in a 30-cycle reaction (5 cycles used on PCR products), employing the AllTaq Master Mix Kit (Qiagen, USA)
SUB15795343
WT.VNAM.12.female