Bm_rna_MA_F_1 (CRX1174892; CRA018601)
After extracting total RNA, mRNA was enriched using Oligo (dT) magnetic beads and fragmented at high temperature. The fragmented mRNA was used to synthesize first-strand cDNA, followed by second-strand cDNA synthesis. The cDNA was purified, end-repaired, and an "A" base was added to the 3' end for adapter ligation. After fragment size selection, PCR amplification was performed.
NGDC
CRX1174892
Zhejiang A and F University
DRX802821