EecSeq of Amphiprion percula larva (brood 33, individual 02) for GWAS
This study utilized expressed exome capture sequencing (EecSeq) to target and sequence the expressed regions of the genome in 159 Amphiprion percula larvae. RNA was extracted from the larvae, and complementary DNA (cDNA) libraries were generated using the isolated mRNA. Biotinylated probes were designed based on the cDNA to specifically hybridize to the genomic DNA exons corresponding to the expressed genes. The hybridized fragments were captured using streptavidin-coated beads, followed by PCR amplification to enrich the target regions. Sequencing libraries were pooled and sequenced on an Illumina NovaSeq SP flow cell lane, generating paired-end reads to facilitate the analysis of the captured expressed exome.
SUB14793258
APPC_33.02_EecSeq