PromethION sequencing
The library design aimed to sequence full-length MPXV genomes directly from cultured isolates using long-read Nanopore sequencing. Rapid barcoding was applied for multiplexing, and libraries were sequenced without fragmentation or enrichment.
Libraries were constructed using the Oxford Nanopore Rapid Barcoding Kit V14 according to the manufacturerï¿½s protocol. No fragmentation was performed. Barcoded libraries were loaded directly onto a PromethION 2 Solo system with R10.4.1 flow cells.
Helmholtz Institute for One Health
ena-EXPERIMENT-TAB-07-08-2025-10:58:06:559-40729