RNA-seq of Scylla paramamosain: male testis
After total RNA extraction from the sample, the mRNA was enriched by removing rRNA using a conventional kit. We further reverse transcribed the enriched mRNA to form double-stranded cDNA. After repairing the double ends of the cDNA, the adaptor was added and the library was constructed by PCR amplification
SUB15092777
EGFP--1