WTCS+TNBS-F158 (CRX1847795; CRA027654)
RNA from tissues was extracted using the peqGOLD total RNA kit (VWR International). RNA integrity and quantity were assessed using the Nanodrop. Qubit RNA HS assay and the Bioanalyzer 2100 system (Agilent Technologies). A total amount of 1 microg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample. PCR was performed with Phusion High-Fidelity DNA polymerase (New England Biolabs), Universal PCR primers and Index Primer. PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using PE Cluster Kit cBot-HS (Illumina) according to the manufacturer's instructions. After cluster generation, the library preparations were sequenced on an Illumina platform and paired-end reads were generated.
NGDC
CRX1847795
Sichuan University
DRX796693