Jeevamrut_CJ_R8
For shotgun metagenomics, initial DNA (350-500ng input) was validated for concentration using a Qubit 3.0 fluorometer (Thermofisher #Q33238) with a DNA HS assay kit (#Q32851), purity on a Nanodrop 2000, and integrity via agarose gel. Libraries were then prepared using the KAPA Hyper Plus Kit (KR1145-v8.21). Final library validation for size was performed on an Agilent 2100 bioanalyzer with an Agilent high sensitivity DNA assay kit. Sequencing was completed using an Illumina Novoseq 6000 S110 Flow Cell.
Indian Biological Data Centre
Jeevamrut_CJ_R8-23461