biomodal evoC sequencing of cfDNA derived from a CONTROL individual
10ng of each cfDNA sample was end-repaired, A-tailed, and ligated to hairpin adapters. Adaptors were digested and the 3 hydroxyl group was extended by a DNA polymerase to generate molecules with the original sample DNA strand connected to its (copied) complementary strand. Y-shaped sequencing adapters were then ligated to molecules. Methylation at 5mC was enzymatically copied across the CpG unit to the C on the copy strand using a DNA methyltransferase, whereas 5hmC was enzymatically glycosylated to prevent such a copy. 5mC on both the original and copy strands was then protected from subsequent deamination. Unmodified Cs were then deaminated to uracil, subsequently read as thymine.
SUB15720472
CEG1966-AM01-D2512-008