survey of Thunnus alalunga: adult male muscle
Genomic DNA (gDNA) was extracted from the muscle according to the instruction of Blood & Cell Culture DNA Kit (Qiagen, 13323). Samples are first lysed (tissue samples are mechanically disrupted) and proteins simultaneously denatured in the appropriate lysis buffer. Protease is then added and after a suitable incubation period, lysates are loaded onto the QIAGEN Genomic-tip. DNA binds to the column while other cell constituents pass through. Following a wash step to remove any remaining contaminants, pure, high-molecular-weight DNA is eluted and precipitated with isopropanol. Finally, DNA quality was analyzed using NanoDrop (Thermo Fischer Scientific, ND2000), Qubit (Invitrogen, Q33238), and electrophoresis.
SUB16273023
ALB_WGS