mRNA profiling of the sheep carotid body and superior cervical ganglion across development
mRNA profiling of the sheep carotid body and superior cervical ganglion across development
Tissues were dissected as described in (https://www.biorxiv.org/cgi/content/short/2025.08.21.671502v1) and snap-frozen in liquid nitrogen. The frozen samples were lysed in RNA lysis buffer (Qiagen) and refined to a fine suspension using a hand-held motorised homogeniser with a 5 mm probe (Pro-Scientific). RNA extraction was performed using the RNA Clean & Concentrator-5 kit (Zymo). The integrity of RNA was assessed by Agilent Bioanalyzer. Strand-specific RNA-seq libraries were prepared using the KAPA RNA HyperPrep Kit with RiboErase (Roche), which depletes ribosomal RNA prior to library construction. Libraries were quality-checked and quantified before pooling.
European Bioinformatics Institute
E-MTAB-17285:CB_fetal_d145_2b_p