GSM9834280: 8-week untreated control mouse dorsal skin; Mus musculus; RNA-Seq
Three animals were pooled per condition. Immediately after collection, tissues were enzymatically dissociated using the gentleMACS Whole Skin Dissociation Kit (Miltenyi Biotec, Bergisch-Gladbach, Germany) according to the manufacturer's instructions on a gentleMACS Octo Dissociator (Miltenyi Biotec). Cell suspensions were filtered sequentially through 100 µm and 40 µm strainers and washed in PBS containing 0.04% bovine serum albumin (BSA; Sigma-Aldrich, St. Louis, MO, USA). Cell viability and concentration were assessed with the Acridine Orange/Propidium Iodide (AO/PI) Cell Viability Kit (Logos Biosystems, Anyang-si, Gyeonggi-do, Korea) on a LUNA-FL Dual Fluorescence Cell Counter (Logos Biosystems), and the concentration was adjusted to 0.7–1.2 × 10⁶ cells/ml. Gel beads-in-emulsion (GEMs) were generated, and GEM formation, barcoding, clean-up, cDNA amplification, and library preparation were performed according to the manufacturer's instructions using the Chromium Next GEM Single Cell 3′ GEM, Library and Gel Bead Kit v3.1, the Chromium Next GEM Chip G Single Cell Kit, and the Single Index Kit T Set A (10x Genomics, Pleasanton, CA, USA). Sequencing, demultiplexing, and read counting were performed by the Biomedical Sequencing Facility of the Center for Molecular Medicine (CeMM, Vienna, Austria).
GEO
GSM9834280_r1