GSM9317258: EHEC wild type replicate 1; Escherichia coli O157:H7 str. Sakai; RNA-Seq
Total RNA was extracted from E. coli O157:H7 str. Sakai using a guanidinium thiocyanate–phenol method (Tollervey D, Mattaj IW. 1987. Fungal small nuclear ribonucleoproteins share properties with plant and vertebrate U-snRNPs. Embo j 6:469-76.) Total RNA-seq library contruction was performed at Novogene (Singapore). From the supplier: "ribosomal RNA was removed from total RNA, followed by ethanol precipitation. After fragmentation, the first strand cDNA was synthesized using random hexamer primers. During the second strand cDNA synthesis, dUTPs were replaced with dTTPs in the reaction buffer. The directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification."
GEO
GSM9317258_r1