GSM9323932: eWAT, HFD, Control,rep 2; Mus musculus; RNA-Seq
[ex vivo adipose tissue macrophages] For bulk RNA-seq of sort-purified cell populations, RNA was isolated using standard Trizol protocol.  [whole eWAT] Total RNA was isolated from snap-frozen tissue samples. RNA extraction was performed using the Maxwell RSC simply RNA Tissue Kit (Promega, AD1340). Briefly, tissue was homogenized in a Thioglycerol Homogenization Solution using the Maxwell RSC Instrument (Promega). [ex vivo adipose tissue macrophages] For bulk RNA-seq of sort-purified cell populations, libraries were prepared using the SMARTer Stranded RNA-seq kit (Clontech) and TruSeq RNA library prep kit (Illumina).   [whole eWAT] RNA libraries were prepared using the standard Illumina protocol.
GEO
GSM9323932_r1