GSM8766860: HeLa_DAB_treated_00H; Homo sapiens; RNA-Seq
No RNA extraction Libraries were prepared using the NEGEDIA NGD0050R_single-cell RNAseq (10X Genomics) Sequencing service (Negedia s.r.l). In brief, single cells were suspended in phosphate‐buffered saline containing 0.04% bovine serum albumin, filtered using 40 um cell strainer (Biologix), and their concentration was evaluated at LUNA-II™ Automated Cell Counter (Logos Biosystems). The cell suspension was loaded onto the Chromium Single Cell G Chip Kit (10x Genomics) and run on the Chromium Single Cell Controller (10x Genomics) to generate single‐cell gel beads emulsion, according to the manufacturer's protocol. The single‐cell 3′ Library and Gel Bead Kit V3.1 (10x Genomics) was used to generate cDNA and the final libraries. The cDNA quality was assessed using High sensitivity D5000 screentape on Agilent 4200 TapeStation system (Agilent Technologies). Quality of libraries was assessed by using screen tape High sensitivity DNA D1000 (Agilent Technologies). Finally, the libraries were sequenced on Novaseq6000 sequencer (Illumina) according to manufacturers' specifications.
GEO
GSM8766860_r1