GSM8972067: Extracellular RNA derived from dying SaOS-2 cells  (RNA pulled from 5 biol rep each of 3 technical rep); Homo sapiens; RNA-Seq
To collect RNA released from dying cells (RNA D), U-2 OS, SaOS-2, MG-63, and 143B cells were treated with 15, 10, 20, and 10 µM etoposide for 72 h, respectively. To collect RNA released from senescent cells (RNA S), U-2 OS, SaOS-2, MG-63, and 143B cells were treated with 1, 1, 5 and 2 µM etoposide for 24 h, respectively, and left in the culture for additional 7 days. Cell culture medium was then collected, cellular debris were removed from cell culture medium by centrifugation (300xg, 5 min, RT) and RNA D and RNA S were extracted from supernatants using acid phenol: chloroform: isoamyl alcohol (125:24:1, pH 4.5) solution according to the manufacturer's instructions (AM9722, Thermo Fisher Scientific, Waltham, MA, USA). The purity and concentration of isolated RNA were analyzed using NanoDropTM 2000 UV-Vis spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). Analyzed RNA was pulled from 5 biol rep samples each of 3 technical rep. RNA samples were kept at -86 °C until further use (stimulation analysis). Library was prepared from 50 ng of total RNA with the NEBNext®UltraTM II Directional RNA Library Prep Kit for Illumina® (NEB) libraries
GEO
GSM8972067_r1