GSM8973242: Inguinal stromal vascular fraction cells isolated from global Mgp deletion mice; Mus musculus; RNA-Seq
Stromal vascular fraction (SVF) cells from inguinal (subcutaneous) white adipose tissue were isolated, using a modification of a previously described methodology. Briefly, the inguinal fat was dissected, minced, and digested in 0.1% (w/v) collagenase II solution (Sigma, #C6885) at 37°C for 30 min with gentle agitation. DMEM supplemented with 20% FBS was used to stop the digestion. The digested adipose tissue was filtered through a 100 µm-cell strainer and centrifuged at 500 rpm for 5 min. The pelleted layer included the SVF cells, and blood cells, which were disrupted using Erythrocyte lysis buffer. The remaining SVF cells were washed twice with FACS buffer (5 mm ethylenediaminetetraacetic acid (EDTA) and 2% FBS in Dulbecco's Phosphate-Buffered Saline (DPBS), pH 7.4).  After dead and imuno cells were removed by DAPI and Cd45 staining using FACS, live SVF cells were sent for scRNA-sequencing. Library was contructed per 10X Genomics instructions
GEO
GSM8973242_r1