GSM9179400: HL60, Control_1; Homo sapiens; RNA-Seq
Eurofins Genomics performed RNA extraction.Total RNA was used as starting material. For eukaryotic samples using polyA selection, 150 ng of total RNA was used (up to 25 µl, with an optimal concentration of 6–50 ng/µl and a maximum of 200 ng/µl). For both eukaryotic and prokaryotic samples using rRNA depletion or mRNA enrichment, 20 ng of RNA was used (up to 15 µl, at a concentration above 1.4 ng/µl). Eurofins Genomics prepared a library for us. Library preparation included purification of poly-A mRNA (if required), followed by mRNA fragmentation and strand-specific cDNA synthesis using random primers. Adaptors were ligated, and the libraries were amplified by adapter-specific PCR. Sequencing was performed on the Illumina platform using paired-end 150 bp reads, with a guaranteed yield of 30 million read pairs (±3%).
GEO
GSM9179400_r1