Whole exome sequencing of homo sapiens: whole blood
DNA was extracted from whole blood using the DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany). Whole exome sequencing was performed with the Twist Human Comprehensive Exome Kit (Twist Bioscience, San Francisco, USA) using 50 ng of genomic DNA isolated from whole blood as input. Exome-enriched libraries were sequenced as 2 x 150 bp paired-end reads on a NovaSeq 6000 system (Illumina, San Diego, USA) to an approximated 100 x coverage of the target region.
SUB14752178
GE_GC_290