GSM9472986: G361_WT_2; Homo sapiens; RNA-Seq
Cells were collected during exponential growth phase, pelleted, and frozen at -80°C until use. Total RNA was extracted using the Qiagen RNeasy Plus Mini Kit with QiaShredder for homogenization per manufacturer's protocol. RNA was quantified using BioTek Synergy H1 multimode plate reader with Take3 plate. To assess the integrity and quality of the RNA, we used the Qubit RNA IQ Assay by ThermoFisher (#Q33222) Samples were prepared and sequenced on the Illumina NovaSeq PE150 platform using standard Novogene protocols. Library construction was perfomed by Novogene using NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® for all the samples.
GEO
GSM9472986_r1