GSM9324136: NC1; Mus musculus; RNA-Seq
Single-cell RNA sequencing was performed on tumor tissues formed by ovarian cancer cells (n = 5 tumors per group, pooled). Fresh tumors were dissociated at 37°C for 40 min in RPMI-1640 medium containing 0.5 mg/mL DNase I, 0.5 mg/mL Hyaluronidase, and 1 mg/mL Collagenase B. Immune cells were subsequently isolated using CD45-positive selection nanobeads (BioLegend, 480028) to achieve a high-purity population (~80%). After enrichment of viable cells by cell sorting, the samples were loaded onto a 10× Genomics Chromium Controller. scRNA-seq libraries were constructed following the manufacturer's protocol for the Chromium Single cell 3'Library and Gel Bead Kit V3.1 (10× Genomics, PN1000268).
GEO
GSM9324136_r1