IDC24
Total RNA was depleted of rRNA using the Ribo Zero Gold Kit before library preparation using the NEBNext Ultra RNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina. Following adaptor ligation, PCR amplification of the DNA was performed over 12 cycles. DNA purification was then performed using Agencourt AMPure XP.
Saitama Medical University
DRX771624