GSM9085101: MCF-7, estradiol treatment, replicate 2; Homo sapiens; ATAC-seq
MCF7 cells were cultured and treated as outlined for CUT&Tag experiments. For chromatin accessibility studies, we used the ATAC-seq kit from Active Motif (#53150), following the manufacturer's instructions. In brief, cells were harvested, counted, and assessed for viability. We processed 100,000 viable cells per condition (N=3), lysing them to isolate nuclei. Tagmentation was carried out for 30 minutes at 37°C using an hyperactive Tn5 transposase. The tagmented DNA was then isolated with DNA purification columns and amplified through 10 cycles of PCR, employing a dual combination of indexed primers based on Illumina's Nextera adapters. DNA cleanup was conducted using SPRI beads. Finally, DNA concentration was measured with a Qubit (Invitrogen), and library profiles were evaluated using a Tapestation (Agilent). Libraries were normalized, pooled, denatured and loaded with 2% PhiX v3 control spike-in (Illumina) for sequencing on Illumina NextSeq550 sequencer. Sequencing parameters were set to 2 x 38 bp sequencing.
GEO
GSM9085101_r1