WGBS sequencing of Summer_1
Genomic DNA was extracted and fragmented, followed by end-repair, A-tailing, and adapter ligation. Bisulfite conversion was performed using the EZ DNA Methylation Gold Kit (Zymo Research), with a certain proportion of negative control lambda DNA included to estimate bisulfite conversion efficiency. The libraries were size-selected (200-500 bp) and amplified by PCR before sequencing. Paired-end 150 bp sequencing was conducted on an Illumina HiSeq 4000 platform. For further details, please refer to the supplementary file "5.1_LC-Bio WGBS&RRBS Experimental Protocol.pdf".
SUB15093054
Summer_1