GSM8899259: Rat_Kidney_RM_C_DSP-1008220000001-C-G09; Rattus norvegicus; OTHER
4 um Formalin-fixed paraffin-embedded rat kidney sections were used. 132 specific ROIs within 3 rats per condition (Sham, HS, RM, RM-HS) were selected for spatial transcriptomic profiling usins DSP GeoMX Nanostring technology. We manually selected regions of interest (ROIs) from four kidney areas: glomeruli (G), cortex (C), corticomedullary junction (CMJ), and medulla (M). Slides were incubated with GeoMx Mouse WTA (Nanostring) assay probe cocktail overnight. The slides were applied with morphology markers incubation before loading onto GeoMx machine. Library preparations were carried out according to the GeoMx NGS Readout Library Prep Manual (MAN-10117; NanoString). Libraries were sequenced on an Illumina NovaSeq6000 according to the manufacturer's instructions.
GEO
GSM8899259_r1