Mater_KO_MII_100_WGBS_rep2 (CRX2203099)
Oocytes were lysed with 10ul of STEM-seq lysis buffer containing 1ul of protease K for 3 h at 55 C, followed by heat-inactivation for 1 h. Samples were then treated with 1ul of dsDNA Fragmentase for 30 min before being subjected to bisulfite conversion using EZ DNA Methylation-Lightning Kit. Libraries were prepared using the EpiArt DNA Methylation Library Kit according to the manufacturer's instructions. In brief, a 3' adaptor was first added to the converted and denatured ssDNA. After DNA extension and purification, a 5' adaptor was added. dsDNA with both 3' and 5' adaptors were then subjected to PCR amplification and libraries were sequenced using Illumina Hiseq X Ten system. For all samples, lambda-DNA was added with a mass ratio of 1:200 before bisulfite conversion for conversion rate analysis.
NGDC
CRX2203099
The Third Affiliated Hospital of Guangzhou Medical University
DRX1007768