Nanopore sequencing of AmB susceptible, sectorized Aspergillus terreus
The genome of WT and ATSec were sequenced with Oxford Nanopore Technologies (ONT). Guppy (v. 4.2; Oxford Nanopore Technologies) was used for base calling, adapter removal and quality verification. The 2 nanopore read bundles were then assembled using the Flye assembler in Galaxy (v. Galaxy Version 2.6) using the Nanopore Corrected mode and the 35m genome size setting. The Quast assembly quality tool in Galaxy (v. Galaxy Version 5.0.2+galaxy1) was used to determine the quality of the assemblies. The estimated genome size setting was 35,000,000 and the organism type was Fungus. Any options not specified were left at default.
SUB14714546
ATSec