GSM8553841: Cow5, 300OA; Bos taurus; RNA-Seq
media was removed, wells were rinsed with 200 µL 1X PBS, then 200 µL of RNA lysis buffer was added. RNA extraction and purification was performed using Maxwell RSC simplyRNA Cells Kit (Promega; AS1390) in the Maxwell Instrument. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library. For the non-directional library, it was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. For the directional library, it was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification
GEO
GSM8553841_r1