GSM8660030: HeLa, non-treated, Input; Homo sapiens; RIP-Seq
For YTHDC1-CLIP, cells were crosslinked by UV and processed with cell lysis and RNase T1 digestion. After immunoprecipitation, the immunocomplexes were digested with protease K and the bound RNAs were purified by using RNA Clean & Concentrator-5 kit (Zymo Research). For m1A-DIP, cells were processed with nuclear extraction and then genomic DNA purification by using DNeasy Blood & Tissue Kit (Qiagen). For MapR, the genomic DNAs were recovered from cell lysates by using MiniElute® PCR purification kit (Qiagene #28004) after digesting with the MNase-conjugated proteins. NEBNext Ultra DNA library kit (for m1A-DIP)
GEO
GSM8660030_r1