GSM9178738: Naïve-2, untreated; Oryctolagus cuniculus; ATAC-seq
After clinical evaluation and on day14 post-injury, corneal tissue was collected and subjected to ATAC sequencing commercially by Activ Motif. 30-50mg flash frozen tissue was manually disassociated to isolate nuclei. Approx. 100,000 nuclei were tagmented via the enzyme and buffer provided in the ATAC-Seq Kit (Active Motif) The fragmented DNA was then purified via a DNA extraction method and amplified with PCR. The resulting libraries were purified via SPRI beads.
GEO
GSM9178738_r1