GSM8553821: Katnal2 WT cortex; Mus musculus; RNA-Seq
P5 cortical tissues were treated first by 0.25% Trypsin-EDTA at 37℃ for 30 min, then by 0.2% type-II collagenase for 4 h to dissociate the cells. After removing cell clumps using a 70 μm sterile nylon mesh cell strainer, single cells were isolated using fluorescence-activated cell sorting and encapsulated in droplets along with barcoded beads and reverse transcription reagents using the 10x Genomics Chromium Controller. The 10x Genomics platform uses microfluidic technology to encapsulate beads and cells with Cell Barcode in droplets, collect the droplets containing the cells, and then lyse the cells in the droplets so that the mRNA in the cells binds to the Cell Barcode on the beads to form Single Cell GEMs, in which reverse transcription reactions are performed to construct cDNA libraries, which are sampled on the library sequences Distinguish the sample source of the target sequence.
GEO
GSM8553821_r1