RNA-Seq of RAT: male colon
Total RNA that passed the quality control was used as the starting sample, and the TIANSeq mRNA capture kit (TIANGEN Biotech) was used to enrich the eukaryotic total RNA with Poly-A structure, and then the captured RNA was used as the starting sample for library construction using the TIANSeq Rapid RNA library construction kit (Illumina platform) (TIANGEN Biotech). TIANGEN Biotech) for library construction. The transcriptome sequencing library was constructed through the steps of RNA fragmentation, cDNA one-strand/two-strand synthesis, end repair, A-tail addition, junction ligation and PCR library enrichment.
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