transcriptomic sequencing
In this project, the mRNA with polyA structure in the total RNA was enriched through Oligo(dT) magnetic beads, and the RNA was broken into fragments with a length of about 300bp by ion interruption. Select the fragment with a length of 304bp. This is because the length of the joint is fixed. If the length of the broken fragment is short, it will lead to a higher proportion of the joint sequence, thereby reducing the proportion of valid data. If the length of the interrupted fragment is relatively long, it is not conducive to clustering during the sequencing process on the machine
SUB15302328
Me2