GSM8519117: GM02036 Control biol rep 1; Homo sapiens; ssRNA-seq
Cells were lifted by 0.25% Trypsin-EDTA, quenched with conditioned culture media, and washed with 1x PBS. Total RNA was then isolated using the RNEasy kit (Qiagen) per manufacturers protocol. Poly-A(+) RNA enrichment, and strand-specific library preparation were carried out using a Tecan/Nugen Universal Plus mRNA Kit. Paired-end 150 bp sequencing was carried out on an Illumina NovaSeq 6000 instrument by the University of Colorado Cancer Center Genomics Shared Resource.
GEO
GSM8519117_r1