GSM9472967: NF1 HGG17 Allo wt Rep1; Mus musculus; RNA-Seq
Nuclei isolated using Chromium Nuclei Isolation Kit (10X Genomics, 1000494). 6-10mg of brain tissue was dissociated 20-60 times with a chilled pestle in lysis buffer, centrifuged at 16000g, and resuspended in wash/resuspension buffer (1X PBS+1% BSA+1X RNase Inhibitor). Single-nuclei suspensions processed using 10X Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (Dual Index). Library fragment size determined via Agilent Bioanalyzer 2100; quantified using qPCR-based KAPA quantification kit.
GEO
GSM9472967_r1