GSM8553111: MDV infected, replicate 1, scRNA seq; Gallus gallus; RNA-Seq
Whole spleens were subsequently collected for each bird, and splenic-derived leukocytes were isolated. Briefly, individual spleens were homogenized and filtered through a 40µM cell strainer to obtain single-cell suspensions. Mononuclear leukocytes were enriched with a density gradient cell separation protocol using Histopaque-1077 medium (MilliporeSigma, Burlington, MA). Six single-cell suspensions (2 x 10^5 cells from 3 control and 3 MDV-infected samples) were transferred the same day on ice for sequencing. Chromium Next GEM Single Cell 3' kit v3.1 was used to prepare the single-cell libraries on the 10X Genomics platform (10X Genomics Inc., Pleasonton, CA) with a target number of 10k cells per sample. The 10X single-cell libraries were sequenced on the NovaSeq 6000 system (paired-end 28bp x 90 bp) (Illumina, San Diego, CA).
GEO
GSM8553111_r1