Shotgun reads of Alvinella pompejana gut metagenome
We used a proximity-ligation approach (ProxiMeta HiC) to optimize the sequencing of entire bacterial genomes directly from the microbiome without requiring high molecular weight DNA to perform sequencing.49 Samples were extracted, assessed for quality control, and sequenced on an Illumina HiSeq with 150bp reads. Construction of accurate metagenome-assembled genomes (MAGs) and functional profiling of the gene content requires intensive bioinformatics pipeline planning and implementation, and we followed recently published guidelines
SUB15958146
Ap_R1