GSM9324879: Multiome, T cells, Infant 3, ATAC; Homo sapiens; ATAC-seq
Nuclei were isolated from infant and adult spleen samples following the Chromium Next GEM Single Cell Multiome ATAC + Gene Expression sample preparation workflow (10x Genomics). Tissue was dissociated, lysed, filtered, and washed according to the manufacturer's instructions to obtain clean, intact nuclei suitable for Multiome library preparation. Single-nucleus Multiome ATAC and gene expression libraries were prepared using the Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Kits (10x Genomics). Library construction followed the standard workflow, including GEM generation and barcoding, post-GEM cleanup, amplification, and construction of separate indexed ATAC and gene expression libraries for sequencing on an Illumina platform.
GEO
GSM9324879_r1