GSM9797013: PAECs_NC_rep1; Homo sapiens; RNA-Seq
Total RNA was extracted using the TRIzol reagent (Invitrogen) following the manufacturer's instructions. RNA concentration and purity were assessed using a NanoDrop 2000 spectrophotometer (Thermo Scientific), and integrity was verified by agarose gel electrophoresis and an Agilent 2100 Bioanalyzer with the RNA 6000 Nano Kit (Agilent Technologies). Strand-specific RNA-seq libraries were constructed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Briefly, mRNA was enriched from 1 μg total RNA using Oligo(dT) magnetic beads, fragmented, and reverse-transcribed into cDNA. After end-repair, A-tailing, and adapter ligation, 400-500 bp fragments were selected using AMPure XP beads, amplified by PCR, and purified. Library quality was evaluated using an Agilent 2100 Bioanalyzer with the High Sensitivity DNA Kit, and concentration was quantified using the PicoGreen assay and qPCR. Libraries were sequenced on an Illumina platform in paired-end 150 bp mode.
GEO
GSM9797013_r1