Transcriptional profiles of feeder-free extended pluripotent stem cells (ffEPSCs) converted from human ESCs, sequenced using the Smart-seq2 protocol
H9 ESCs were induced to become ffEPSCs using LCDM-IY medium with specific inhibitors. The ffEPSCs were then processed into single-cell RNA libraries with the Smart-seq2 protocol, including cell lysis, mRNA capture, cDNA synthesis, and library preparation. Sequencing was conducted on the Illumina HiSeq 2000
SUB14768881
ffEPSCs