GSM8728862: HCT116_sc_0h; Homo sapiens; RNA-Seq
For bulk RNA extraction, 1×10^6 to 5×10^6 cells were fully lysed in 500 μL of RNA-easy buffer (Vazyme, catalog no. R701-01) following the manufacturer's protocol. For single-cell experiments, cells are loaded into the Well-Paired-Seq chip (Decoder Cartridge; Dynamic Biosystems, 2203106) for library preparation using the DECODER single cell 3' kits (Dynamic Biosystems, 2203206, 2203306, 2203406) with an additional step of Dual TimeLapse chemistry treatment before reverse transcription. For bulk assays, the sequencing library was constructed using the TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme Biotech, catalog no. TD502-01) according to the manufacturer's instructions. For single-cell experiments, the 3'-end enriched sequencing library was prepared by the reagents in DECODER single cell 3' kits (Dynamic Biosystems, 2203306, 2203406) without further modifications.
GEO
GSM8728862_r1