GSM9834524: Colon crypts from wild-type mouse, replicate 3; Mus musculus; RNA-Seq
Total RNA was isolated from colon tissue samples using QIAzol lysis reagent (79306, Qiagen, Germany). Genomic DNA was removed by DNaseI (Roche, Germany) according to the manufacturers' recommendations. RNA concentration was analyzed using a NanoDrop 2000 spectrophotometer (ThermoScientific, USA). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis. After end repair, A-tailing, adapter ligation, size selection, amplification, and purification, the library was completed. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries were pooled and sequenced on an Illumina platform according to effective library concentration and data amount, and paired-end reads were generated.
GEO
GSM9834524_r1