ITS metabarcoding sediment
DNA was extracted from each homogenized sediment sample using the Soil DNA Isolation Plus kit (Norgen Biotek). The sequencing procedure followed the Fungal MetagenomicSequencing Demonstrated Protocol (Illumina, San Diego, CA,USA), adapted for metabarcoding by amplifying the ITS86ITS4region of the fungal rRNA gene cluster (Waud et al., 2014; Garces-Fiallos et al., 2022). Briefly, amplicons were generated, prepared forsequencing following standard library preparation steps, andsequenced on the Illumina MiSeq System with 2  301 bp pairedendreads. Raw data were stored in FASTQ format and uploaded tothe Illumina BaseSpace Sequencing Hub for further analysis.
SUB15957790
2005L-73