GSM8695085: IL-7Rsup-hi rep 1 (RNA-seq); Mus musculus; RNA-Seq
Mouse splenocytes were harvested and enriched by CD4 T cell isolation kit (#130-104-454, Miltenyi Biotec) in negative selection. Then using the enriched CD4+ T cells, naive CD4 T cells (TCR-β+, CD4+, CD25-, CD62Lhi, CD44low, IL-7Rsup-hi or low) were sorted using FACS (ARIA Fusion, BD Bioscience). RNA was extracted using a Quick-RNATM MicroPrep kit (ZYMO RESEARCH). Total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using TruSeq mRNA-Seq Library Prep Kit following manufacturer's protocols.
GEO
GSM8695085_r1