RNA-Seq of biofilm on  water incubated in TWW
200 mL water samples were filtered through 0.45 um polycarbonate filter membranes (Millipore Sigma). DNA was extracted from filters using a DNeasy PowerLyzer Power Soil kit. 16S rRNA libraries were prepared with primers targeting the V4 region. Each template was amplified in triplicate, pooled, and cleaned using a Qiagen Qiaquick 96 Purification Kit. The samples underwent dual indexing PCR to ad adapters and indexes for runs on an Illumina MiSeq using paired-end (250) sequencing.
SUB14786314
ESF21MP-5-2-0