GSM9769940: HCR sedentary aged soleus RNA-seq H2; Rattus norvegicus; RNA-Seq
Rats were deeply anesthetized, and soleus muscle samples were rapidly excised, snap-frozen, and stored at -80 C. Total RNA was extracted from soleus muscle using the Quick-RNA Miniprep Plus Kit (Zymo Research) according to the manufacturer's instructions. RNA-seq libraries were generated using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD v2 for Illumina (Lexogen). Briefly, 100 ng of total RNA was used for first-strand cDNA synthesis using an oligo(dT) primer, followed by RNA removal. Second-strand synthesis was initiated by random priming, and products were purified using magnetic beads. Libraries were amplified and barcoded by PCR, quality assessed using an Agilent 4200 TapeStation, and sequenced to generate single-end reads.
GEO
GSM9769940_r1