GSM9694458: Sample_2714206; Homo sapiens; RNA-Seq
RNA was extracted using the Covaris truXTRAC® FFPE total NA kit; RNA quality was assessed using bioanalyzer. Total RNA samples were converted into cDNA sequencing libraries with the "QuantSeq 3' mRNA-Seq Library Prep Kit (FWD) for Illumina" (Lexogen, Cat.No. 015). Briefly, library generation is initiated by reverse transcription with oligodT priming, followed by a random-primed second strand synthesis. A UMI Second Strand Synthesis module was used, whereby random primers featured a 6 nt long Unique Molecular Identifier 5' tag to be addressed at data analysis. Primers from both steps contain Illumina-compatible sequences. Libraries were completed by PCR, and sequenced on an Illumina instrument (see below) by following manufacturer's protocols.  {This library preparation kit generates directional libraries stranded in the sense orientation (the read1 (the only read in single read format) has the sense orientation (--library-type fr-secondstrand in TopHat, --stranded=yes in HTSeq)}
GEO
GSM9694458_r1