GSM8695681: imb_keller_2023_16_07_SoaKI_4h_IgG_Rep1; Anopheles gambiae; OTHER
The experiment was performed with flash frozen tissues, which were homogenized in cold PBS and passed through a cell strainer. The cell suspension was counted and 0.4 Mio cells were used for each reaction. 100 000 cells were used for each reaction. The libraries were generated according to the following protocol: dx.doi.org/10.17504/protocols.io.bcuhiwt6). Antibodies are listed with the respective samples. We used pA-Tn5 prepared by the IMB protein production core facilty. CUT&Tag libraries were amplified using NEBNext Ultra II Q5 Master Mix with standard protocols and 15 PCR cycles. Libraries were cleaned up using 1.3 volume Ampure XP beads and pooled together for paired-end sequencing.
GEO
GSM8695681_r1