whole genome sequencing of SN11B (CRX1161909)
Samples meeting the quality criteria were subjected to genomic DNA fragmentation and size selection. The fragments were then end-repaired, adenylated to add an "A" tail, and ligated with specific adaptors. The resulting products were amplified via ligation-mediated PCR. PCR products were purified, and the library was amplified using phi29 to create DNA nanoballs (DNBs). These DNBs were loaded onto a patterned nanoarray, and paired-end 100/150 base reads were generated through combinatorial Probe-Anchor Synthesis (cPAS). Sequencing was conducted on DNBSEQ-T7 platform, yielding 150bp paired-end reads.
China Agricultural University
DRX993546
NGDC
CRX1161909