197C
Nanopore sequencing was performed using an SQK-LSK114 Ligation Sequencing Kit with EXP-PBC096 barcoding on a PromethION FLO-PRO114M flowcell through Auckland Genomics (University of Auckland, Auckland, New Zealand). Real-time base-calling was done using a high-accuracy model with Guppy 7.1.4. Adaptors were trimmed but barcodes were not. After the run was complete, the reads were re-base-called using a Super High accuracy model with Dorado 0.5.3 and model dna_r10.4.1_e8.2_400bps_sup@v4.2.0. Quality checking of sequences was performed with FastQC
SUB15923279
197C