Urine cfDNA
Urine cfDNA was extracted using the MagMax Cell-Free DNA Isolation Kit (Applied Biosystems) and 1ng was prepared into a library using a single-stranded library preparation technique (Kapp et. al. 2021). with the modifications described in the supplemental of Nguyen et al. 2023. Libraries were sequenced on a 2x61 run on a Novaseq 2000 and reads were aligned to GRCh38.
NHGRI-phs004104
U007