GSM8767159: AD_negative (R-, AD- and R+, AD-) HTO; Mus musculus; OTHER
Hash tag antibody (Biolegend) labeled nuclei were isolated using Fluorescence Activated Nuclear sorting (FANs) to enrich for microglia nuclei by preferentially excluding NeuN (neuron), Sox10 (Oligodendroglia) and Sox9 (astrocyte) nuclei and nuclei were mixed in equal proportions to generate Group 1 (SN1-4) and Group 2 (SN5-SN8) samples for nuclear capture Group1 and Group2 libraries were generated from sample nuclei (SN1-4 and SN5-8) that were mixed together in equal proportions and captured using a Chromium Chip G microfluidics chamber and a Chromium X series processor and the Chromium Next GEM 3' kit as specified by the manufacturer (10X Genomics). The cDNA libraries were processed for compatibility with Illumina sequencing using the 3' Feature Barcode Kit, the Dual Index Kit NT Set A, and the Dual Index Kit TT Set A according to the manufacturer's specifications (10X Genomics)
GEO
GSM8767159_r1