Mannan-Rich Fraction Supplementation in Sows Modulates Fecal Microbiome and Colostrum and Supports Piglet Growth
Whole DNA fractions were fragmented by sonication to the size of ~350 bp. The DNA fragments were then end-polished, A-tailed, and ligated to a full-length Illumina adaptor with PCR amplification. Each PCR product was purified (AMPure XP system), and library size distributions were established using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa-Clara, CA, USA) and quantified 1.5nM through Qubit (Thermo Fisher Scientific) and qPCR. A clustering of the uniquely indexed samples was performed on the Illumina cBot Cluster Generation System; then, the library preparations were sequenced on an Illumina NovaSeq 6000 platform, using an S4 flow cell with paired-end 150 bp reads (Novogene, Cambridge, UK).
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