GSM8834621: Citeseq, scRNA-seq, CD45+ lung cells, tube 2; Mus musculus; OTHER
Lungs were digested with Liberase TL (33.3 μg/ml) and Dnase I (58 μg/ml), shaken at 37C for 30 minutes, mashed through 70 um filters, treated with ACK lysis buffer, washed and resuspended in antibody mix, washed again and resuspended, and sorted. Library preparation was performed according to manufacturer's instructions using 10x Genomics kit version
GEO
GSM8834621_r1