DNA-Seq of Aspergillus niger DFA-N - Nanopore
DNA was extracted from mycelium grown on Petri dish using the Qiagen DNeasy 96 PowerSoil Pro QIAcube HT Kit (#47021).Prior to extraction, samples were lysed using MagMAX Microbiome bead beating tubes. An additional lysis step was included: 20U of Zymo's Zymolyase Ultra was added and incubated at 37C for 1 hour prior to bead beating.  Whole genome DNA sequencing library for the sample was prepared using the Oxford Nanopore Technologies SQK-NBD114 native barcoding kit. Long Fragment Buffer was used to promote longer read lengths. Sequencing was performed on the GridION platform using a FLOW-MIN114 Spot-ON Flow Cell, R10 version with a translocation speed of 400bps. Base calling was performed on the GridION using the super-accurate basecalling model.Versions of the software include MinKNOW version: 23.07.5 and Guppy version: 7.0.9.
SUB15339620
SeqCoast_6119_ONT