16S amplicon sequencing of microbial communities in Stylocordyla borealis
DNA of preserved samples (stored in 96% EtOH) was extracted using the DNeasy Blood and Tissue Kit (Qiagen). After conducting quality checks of the extracted DNA, the V3-V4 variable regions of the 16S rRNA gene were amplified in a one-step PCR (30x cycles: 98 C/30 s - 98 C/9 s - 55 C/1 min - 72 C/1.30 min - 72 C/10 min - hold at 4C) using the primer pair 341F-806R. After verification of the presence of PCR-products by gel electrophoresis, sequencing was conducted on a MiSeq Illumina sequencing platform (Institute of Clinical Molecular Biology, Kiel, Germany).
SUB14752145
16S_ZBN-E