GSM9798891: Trim28 multiome: Trim28 knockout (Trim28-1 sgRNA): ATAC; Mus musculus; ATAC-seq
Tumors were excised, minced, and enzymatically dissociated using Miltenyi tumor dissociation reagents or an equivalent tumor-infiltrating lymphocyte dissociation workflow. Dissociated tumor material was filtered to remove debris and enrich viable lymphocytes. Depending on sample quality, lymphocytes were enriched by Lympholyte-M density-gradient separation and/or magnetic enrichment before flow sorting. Single-cell suspensions were stained with viability dye, Fc block, and surface antibodies to identify live CD8 T cells and distinguish donor NT and Trim28 populations by congenic markers. Sorted donor cells were processed using the 10x Genomics Chromium Single Cell Multiome workflow to generate paired gene-expression and chromatin-accessibility libraries from the same captured cells. Gene-expression libraries captured cDNA, and ATAC libraries captured Tn5-accessibility fragments from the same sorted donor-cell populations. Libraries were sequenced on an Illumina NextSeq 2000.
GEO
GSM9798891_r1