urban soil microarthropods COI metabarcoding: SIN005
Soil microarthropods were extracted with Berlese funnels from soil. DNA was extracted from the microarthropods with the DNeasy PowerSoil Pro Kits (Qiagen), with the addition of an initial incubation with 10 l of Proteinase K at 56 C for 30 minutes. DNA was resuspended in 50 l of nuclease-free distilled water. One blank sample consisting of 1 ml of sterile distilled water were processed together with the samples. Metabarcoding libraries were prepared using the primers and cycles protocol following Leray et al. (2013): mlCOIintF [5 GGWACWGGWTGAACWGTWTAYCCYCC 3] + jgHCO2198 [5 TAIACYTCIGGRTGICCRAARAAYC 3].
SUB15196245
SIN005