GSM8833706: Steroidogenic adrenal cells, SF1-Cre; mTmG, male 15 week old_Iso21; Mus musculus; RNA-Seq
Following dissociation, cells were stained with 2μg/ml DAPI and 5μM Vybrant DyeCycle Ruby (Invitrogen, #V10309). Ruby-positive, DAPI-negative single cells were sorted based on fluorescent properties using the Thermofisher Bigfoot Cell Sorter at the University of Michigan Flow Cytometry Core. Cells were collected in BSA-precoated tubes containing HBSS 1X with 2% FBS and kept on ice. Single-cell droplets with a target capture of 10,000 cells were immediately prepared on the 10x Chromium system Library was performed according the manufacturer instructions with the Chromium Next GEM Single Cell 3′ Gene Expression Library Construction Kit version 3.1
GEO
GSM8833706_r1