GSM9065567: EV2; Homo sapiens; RNA-Seq
Total RNA was extracted using the qEV RNA Extraction Kit (RXT01, IZON Science LTD) according to manufacturer's instructions. RNA separation, detection and quantitation was performed on a 2100 Bioanalyzer (Agilent Technologies). The SMART-Seq mRNA kit from Takara was used for reverse transcription and cDNA amplification according to manufacturer's specifications, starting with 5 ng in average of RNA as input. 200 pg of cDNA were used for library preparation using the Nextera XT kit from Illumina. Library molarity and quality were assessed with the Qubit and Tapestation using a DNA High sensitivity chip (Agilent Technologies)
GEO
GSM9065567_r1