GSM8899179: B04_hipp_F_5xFADHEMI.CLUh2kbKIHO_4mo_12659; Mus musculus; RNA-Seq
Frozen tissues were lysed and homogenized using the TissueLyser II (Qiagen, Germantown, MD). Total RNA was extracted with the RNeasy Mini Kit and treated with the RNase-Free DNase Set on the QIAcube (Qiagen) liquid handling platform. RNA integrity was assessed using the Qubit RNA IQ Assay (Life Technologies), and samples with an RNA integrity number (RIN) of ≥7.0 were selected for cDNA synthesis. cDNA synthesis, amplification, library construction, and sequencing were performed by Novogene (Sacramento, CA) using Illumina NovaSeq and HiSeq platforms with paired-end 150 bp (PE 150) sequencing strategy.
GEO
GSM8899179_r1