GSM9323076: IDH1R132H immortalized human astrocytes, treatment, H3K27me3, replicate 3; Homo sapiens; OTHER
CUT&Tag was performed with 60,000 cells per sample in triplicates. After bead-binding, cells were permeabilized and incubated with primary antibodies directed at H3K4me1 (Active Motif), H3K4me3 (Active Motif), H3K27me3 (Cell signaling) and H3K27ac (Active Motif), followed by secondary antibody incubation (guinea pig anti-rabbit, Antibodies online). For chromatin cleavage and adapter ligation, pAG-Tn-5 (Epicypher) was used. Library preparation was conducted by PCR with barcoded primers. Samples underwent quality control utilizing D1000 Screen Tape on a Tapestation 4200 (Agilent).
GEO
GSM9323076_r1