MinION of soil microbiome (16s): rhizosphere of Cucurbita pepo
The metagenomic DNA from each soil sample was extracted using the DNeasy PowerSoil Pro Kit for soil from QIAGEN, following the manufacturers instruction. DNA concentration was determined by Nanodrop (Thermo Fisher). The V3-V4 hypervariable 16s rRNA region (Primers: 8F 5-AGAGTTTGATCCTGGCTCAG; 1492R 5- GGTTACCTTGTTACGACTT) was amplified. Library preparation was performed according to the methods of Conti et al. (2022).
SUB14729703
Nu_rhizo_16S_3