RNA-Seq of mus musculus: adult female whole blood
Whole blood samples were collected from mice 28 days after the initial immunization.About 2g total RNA of each sample were used for TCR sequencing library preparation using KC-DigitalTM Stranded TCR-seq Library Prep Kit for Illumina 150 (Seqhealth Technology Co., Ltd., Wuhan, China, Cat. No. DT0813-02) following the manufacturers instruction. The kit eliminates duplicationbias in PCR and sequencing steps, by using unique molecular identifier (UMI) of 8 random bases to label the pre-amplified cDNA molecules.The library products corresponding to 250-500 bp were enriched, quantified and finally sequenced on NovaSeq (Illumina).The sequence was mapped to the international ImMunoGeneTics (IMGT) database. And using MiXCR software (version 3.0.3) to obtain V , D, and J fragments, rearrangement, and CDR3 sequences.
SUB14695550
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