GSM9031115: 3rd non-stressed wild-type mouse; Mus musculus; RNA-Seq
ice were terminally anesthetized and decapitated, and the brains were excised into Earl's based salt solution. The brains were dissociated to a single-cell suspension using the Neural Tissue Dissociation Kit (P) (Miltenyi Biotec, 130–092–628) according to the manufacturer's protocol. For myelin removal, Percoll (Cytiva) protocol was performed. CNS myeloid cells were isolated from myelin-free single-cell suspension using CD11b-conjugated beads (Miltenyi Biotec, 130–093-634) according to the manufacturer's protocol followed by depletion with MS columns (Miltenyi Biotec, 130-042-201). The procedure resulted in > 90% CD11b + cells.RNA was produced immediately from isolated brain microglia of 5XFAD and wt mice using RNeasy Mini Kit 50 (Qiagen) according to the manufacturer's instructions. High quality RNA (RNA integrity number [RIN] > 9.3) was extracted from all groups. Libraries were prepared by the Center for Genomic Technologies at the Hebrew University of Jerusalem, using the KAPA Stranded mRNA-Seq Kit (KR0960, Roche) with poly-A selection, according to the manufacturer's protocol. Single-end sequencing was conducted on an Illumina NextSeq 500 machine using 76 cycles. Sequencing depth ranged between 32 and 40 million reads, with an average of 36 million reads per sample.
GEO
GSM9031115_r1