GSM8729406: FGFR1:TACC1 r5; Homo sapiens; RNA-Seq
RNA was extracted from cells using the TRI reagent protocol. A total of 1 µg of RNA material was used per sample for RNA preparation. The mRNA was purified by poly-T magnetic beads, followed by fragmentation and conversation to cDNA. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library. For the non-directional library, it was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina platforms, according to effective library concentration and data amount.
GEO
GSM8729406_r1