GSM8552813: pt_a_pre; Homo sapiens; RNA-Seq
Cell concentrations were adjusted to 1000 cells/μl and cell suspensions were loaded onto a Chromium Next GEM Chip G (10X Genomics) for a target output of 10,000 cells per sample.Single-cell droplet capture was performed on the Chromium Controller (10X Genomics) by Children's Medical Research Institute single cell sequencing centre cDNA library preparation was performed in accordance with the Chromium Next GEM Single Cell 3' v3.1 (dual index) protocol. Libraries were evaluated for fragment size and concentration using Agilent HSD5000 ScreenTape System
GEO
GSM8552813_r1