WGS of RPE-1 cells, HMW, HiFi, Sequel II e
HMW DNA from 1.5 x 106 cells was used to generate PacBio HiFi libraries with the SMRTbell express template prep kit 2.0 (PacBio 100-938-900). Size selection was performed with Megaruptor 2 (Diagenode) with default settings, and fraction sized 15-20 kb as determined by Femto Pulse were sequenced on the Sequel IIe platform with SMRT Cells 8M (PacBio 101-389-001) using the chemistry 2.0 with 2-hour pre-extension, 2-hour adaptive loading and 30-hour movie collection time, to reach a coverage of 46x in PacBio HiFi reads. Circular Consensus Sequencing (CCS) reads were obtained using SMRT-Link (https://github.com/WenchaoLin/SMRT-Link) with default parameters.
SUB15274463
RPE1_HiFi_1