GSM9001619: CD3 PBMCs, lanes 1-2, ADT-derived cDNA; Homo sapiens; RNA-Seq
For sample multiplexing, cells were stained with hashtag oligonucleotides (BioLegend). Acquisition and sorting were performed on a BD FACSymphony S6 Cell sorter. Sorting gates included CD3+ T cells, activated CD4+ and CD8+ T cells (defined as CD38+ HLA-DR+) and CXCR5+ PD-1+ Tfh cells. Sorted cells were processed for ECCITE-seq using the Chromium Next GEM Single Cell 5′ HT Kit v2 (10x Genomics). Cells were pooled, loaded onto Chromium GEM-X Chips, and run on a Chromium controller (10x Genomics). Gene expression, TCR V(D)J, and surface protein expression libraries were made using the 5′ Feature Barcode Kit, the Chromium Single Cell V(D)J Amplification Kit, and the Chromium Next GEM Single Cell 5′ Library Kit (10x Genomics), following the manufacturer's instructions. Libraries were pooled and sequenced using the NovaSeq 6000.
GEO
GSM9001619_r1