GSM9797162: HeLa NXF1-dTAG, DMSO treatment, RNA-seq, biological replicate 1; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol reagent according to the manufacturer's instructions. RNA quality and concentration were assessed prior to library preparation. RNA-seq: 500 ng of total RNA was used for library preparation. Poly(A)+ RNA was enriched using oligo(dT) magnetic beads and fragmented under controlled conditions. First-strand cDNA was synthesized using random primers, followed by second-strand synthesis. Libraries were prepared using the Hieff NGS Ultima Dual-mode mRNA Library Prep Kit for Illumina (Yeasen Biotechnology) with insert sizes of approximately 300-350 bp, purified using AMPure XP beads, and sequenced on the DNBSEQ-T7 platform (150 bp paired-end). 3' end-seq: Libraries were prepared from the same total RNA using the QuantSeq 3' mRNA-Seq Library Prep Kit V2 (Lexogen). First-strand cDNA synthesis was initiated from the poly(A) tail using an oligo(dT) primer containing the sequencing adaptor sequence, followed by second-strand synthesis, amplification, and purification. Libraries were sequenced on the DNBSEQ-T7 platform (150 bp paired-end).
GEO
GSM9797162_r1