GSM9834030: Anopheles gambiae infected with early-selected Vavraia culicis lineage 4 biological replicate 3; Anopheles gambiae; RNA-Seq
Total RNA was extracted from independent pools of 10 adult females using the Qiagen RNeasy Universal Plus kit. Adults were homogenized in QIAzol reagent with stainless steel beads, genomic DNA was removed according to the manufacturer protocol, and RNA was eluted in nuclease-free water. RNA quality was assessed on an Agilent Fragment Analyzer. Libraries were prepared from 250 ng total RNA per sample using the Illumina Stranded mRNA Prep kit with unique dual indices and sequenced as 150 bp single-end reads on an Illumina NovaSeq 6000 platform.
GEO
GSM9834030_r1