16S-seq of rapid sand filter: Mn coated sand, Arsenic Enriched, Inactived with Azide 2
Sequencing libraries were generated usingTruSeq DNA PCR- Free Sample Preparation Kit( Illumina, USA) following manufacturer's recommendations and index codes were added. The library quality was assessed on the Qubit@ 2.0 Fluorometer (Thermo Scientific) and Agilent Bioanalyzer 2 1 0 0 system. At last, the library was sequenced on an Illumina NovaSeq platform and 250 bp paired-end reads were generated.
SUB16055625
N3-2