Human Airway Metagenome - Stenotic Airway - Tracheostomy Tube_4
Samples were collected in the operating room, placed on dry ice, and stored at 80C until processing. DNA was extracted using the QIAamp DNA Microbiome Kit (Qiagen) to enrich microbial DNA and reduce host contamination. Library preparation was performed using the NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs) following standard protocols at the University of Cincinnati Genomics, Epigenomics, and Sequencing Core. Libraries were sequenced on an Illumina NextSeq 2000 with 2  61 bp paired-end reads, targeting ~25 million reads per sample.
SUB15197785
Dion_4