UWR3_YU | Rec:U | Don:R | Wet | r3
Bacterial community profiles were generated by 16S rRNA gene amplicon sequencing (V4V5). Genomic DNA was extracted using commercial soil DNA extraction kits following the manufacturers protocols. DNA integrity was checked by 1% agarose gel electrophoresis, and DNA concentration and purity were assessed using a NanoDrop One spectrophotometer. PCR amplification was performed using barcode-indexed primers and Premix Taq (TaKaRa). Amplicons were quantified (GeneTools Analysis Software v4.03.05.0, SynGene), pooled in equimolar amounts, gel-purified using the E.Z.N.A. Gel Extraction Kit, and eluted in TE buffer. Libraries were prepared using the NEBNext Ultra DNA Library Prep Kit for Illumina and sequenced on an Illumina MiSeq platform. Raw reads were generated by base calling and are provided as paired-end FASTQ files.
SUB15966055
UWR3_YU