Shotgun metagenomics of mixed algal-bacterial culture: reactor 3, control
For DNA extraction, microbial samples were processed using the DNeasy PowerSoil Kit (QIAGEN, Hilden, Germany), following the manufacturers protocol with an additional purification step using phenol:chloroform:isoamyl alcohol (IAA) to increase DNA purity. Total nucleic acid (RNA/DNA) was extracted using the RiboPure-Yeast Kit (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA). Each extract was divided into two aliquots: one reserved for DNA sequencing, and the other subjected to DNase treatment to remove residual genomic DNA and isolate RNA for transcriptomic analysis. The concentration and purity of nucleic acids were assessed before and after DNase treatment using a NanoDrop 2000 spectrophotometer and Qubit fluorometer (Thermo Fisher Scientific).
SUB15849476
control_r1_DNA