Pore-C of Saccharum hybrid POJ2878: young leaf
Pore-C libraries were generated to study multi-way chromatin contacts in Saccharum hybrid POJ2878. In-situ chromatin conformation capture was performed by cross-linking cells with formaldehyde, followed by digestion with HindIII. Interacting DNA fragments were ligated under proximity conditions to form long concatemers. After reversing cross-links, the high-molecular-weight DNA containing multi-fragment ligation products was purified. A sequencing library was prepared using the Ligation Sequencing Kit SQK-LSK114 from Oxford Nanopore Technologies, without further fragmentation. The final library was sequenced on an Oxford Nanopore PromethION platform R10.4 chip to capture long reads spanning multiple interacting loci.
SUB15530546
POJ2878_PoreC_5_0