Lake Constance metatranscriptome study
For metatranscriptome sequencing, messenger RNA was enriched by depleting ribosomal RNA with the Ribo-off rRNA Depletion Kit (Bacteria; Vazyme, Nanjing, China)  to gain higher mRNA concentrations from the samples. The mRNA library was prepared with the TruSeq Stranded mRNA Library Prep (Illumina) and was sequenced on the NovaSeq 6000 sequencer with the NovaSeq SP 300 cycles kit.
SUB15061946
WH20190702