CON3 (CRX1937065; CRA029376)
Total RNA was isolated using the Trizol Reagent. Quality and integrity were determined using a NanoDrop spectrophotometer and a Bioanalyzer 2100 system. Random oligonucleotides and SuperScript III were used to synthesize the first strand cDNA. Then use RNaseH to degrade the RNA strand in DNA-RNA hybrid, and in the DNA polymerase I system, use dNTP with dUTP instead of dTTP as raw material to synthesize the second strand of cDNA.
Dalian Medical University
DRX813221
NGDC
CRX1937065