GSM8695543: K562, lymphoblast, myelogenous leukemia, siGMNN (Geminin siRNA), FANCD2 (Input), Replicate 1; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes and then quenched with 200mM glycine. After cell lysis, chromatin was sonicated using a Bioruptor Pico (Diagenode). ChIP-seq libraries were prepared using ThruPLEX DNA-Seq Kit from Takara Bio. Libraries were end-repaired, adapter-ligated and PCR amplified according to manufacturer's instructions. Next, libraries underwent quality control using a Bioanalyzer with a High Sensitivity DNA chip to assess sample size distributions. Finally, samples were sequenced in a NextSeq550 Illumina platform using a 2x75bp flow cell.
GEO
GSM8695543_r1