seo_micn1 (CRX1284160; CRA020931)
After extracting the total RNA, a quality assessment is conducted to ensure its integrity and purity. Following this, ribosomal RNA (rRNA) is removed from the total RNA to isolate messenger RNA (mRNA). Subsequently, the purified mRNA is treated with fragmentation buffer, which randomly cleaves the mRNA into shorter fragments.The library is constructed via a strand-specific protocol, ensuring that only one strand is selected for sequencing. (Parkhomchuk D. 2009) submitted_instrument_model: Illumina HiSeq X Ten
NGDC
CRX1284160
Shandong First Medical University Shandong Academy of Medical Sciences
DRX823034