eDNA metabarcoding of marine sediment: 18S sequencing
DNA was extracted from 10 g sediment of each sample by using the PowerMax Soil DNA Isolation Kit (QIAGEN, Germany) according to the manufacturers instructions. The primer set, SSU_F04 (5-GCTTGTCTCAAAGATTAAGCC-3) and SSU_R22 (5-GCCTGCTGCCTTCCTTGGA-3), targeting the fragment of the metazoan 18S rRNA gene was used. PCR amplification was conducted in a volume of 20 L with three replicates. The high-throughput sequencing was performed on the Illumina MiSeq platform (300 bp paired-end) by Majorbio Co., Ltd. (Shanghai, China).
SUB15538667
STGY_C01_18S