GSM9065932: B cells, unstimulated A943931, rep3; Homo sapiens; ATAC-seq
Ten thousand live cells were used for each transposition reaction, lyzed, permeabilized and tagmented as described in Corces et al., Nature Genetics 2016 (PMID: 27526324) ATAC-seq libraries were generated according to the FAST-ATAC protocol (Corces et al., Nature Genetics, 2016, PMID: 27526324) with few changes.  The number of PCR amplification cycles was standardized to 13. The libraries were quantified by qPCR using Library Quantification Primer plus KAPA SYBR FAST and pooled at 1 nM. Samples were sequenced using single-index paired-end (75+75 bp) reads on an Illumina® NextSeq 500 instrument in high-output mode.
GEO
GSM9065932_r1