X_tropicalis_hoxc13-KO_forelimb_toe_1
Sequencing libraries from total RNA of the X. tropicalis samples were generated by Novogene Co. (Nanjing, China). Messenger RNA (mRNA) was purified from total RNA using poly-T oligo-attached magnetic beads. Fragmented mRNA was reverse transcribed using random hexamer primers to synthesize first-strand cDNA, followed by second-strand synthesis. Libraries were prepared through end repair, A-tailing, adapter ligation, size selection, amplification, and purification. Libraries were quantified using Qubit and real-time PCR, and size distribution was assessed using a Bioanalyzer. Libraries were sequenced on an Illumina platform NovaSeq X Plus Series generating paired-end 150 bp (PE150) reads. Raw reads ranged from ~29 million to ~100 million per sample.
SUB15698605
Xt_hoxc13KO_forelimb_toe_replicate_1