16S amplicon on soil DNA samples from unplanted pots (control)
The library was prepared using the 16S Barcoding Kit 112 (Oxford Nanopore Technologies), where the full-length 16S rRNA gene was amplified via PCR using barcoded primers (27F/1492R), followed by purification with Agencourt AMPure XP beads. Equimolar amounts of barcoded amplicons (100 ng per sample) were pooled and sequenced on a MinION R9.4.1 SpotON FlowCell, using MinKNOW v3.6.5 for run control.
SUB15049396
ONT_barcode107