RNA-seq of Magallana gigas: adductor muscle from distinct breeding families
Total RNA was extracted from the adductor muscle tissue of Magallana gigas using TRIZOL reagent. Following mRNA enrichment with oligo(dT) magnetic beads, the mRNA was randomly fragmented into short pieces using Fragmentation Buffer under elevated temperature. Double-stranded cDNA was then synthesized and purified. Subsequent library preparation steps included end repair, A-tailing, and adapter ligation. The cDNA fragments were size-selected using AMPure XP beads and amplified by PCR to generate the final sequencing library. The concentration and insert size of the library were assessed using Qubit 2.0 and Agilent 2100, respectively. Paired-end sequencing (PE150) was performed on the DNBSEQ-T7 platform.
SUB15919737
HD1-1