Huh7_TetoffHBV_TetMinus_Rep2
Total RNA was extracted from cells lysed using Trizol (Invitrogen, catalog no. 15596018) followed by Direct-zol RNA miniprep kit (Zymo Research, catalog no. R2052) with in-column DNase I digestion. RNA concentration and purity were assessed by Nanodrop with A260:A280 ratio above 1.9, RNA integrity was evaluated using Agilent Bioanalyzer with RNA integration number (RIN) > 6.0. Samples were submitted to GENEWIZ (Azenta Life Sciences) for library preparation and sequencing. mRNA libraries were prepared using poly(A) enrichment following the service providers standard protocol. Sequencing was performed using Illumina NovaSeq 2x150 bp, with a targeted depth of approximately 30 million reads per sample.
SUB15923422
Huh7-I2