16S rRNA marker gene survey of montaine fishes-Sculpin-intestine
To generate taxonomic and proportional abundance profiles for each sample, the V4 region of the 16S rRNA gene was targeted for amplicon sequencing by polymerase chain reaction (PCR) using the primers 515F (5-GTGYCAGCMGCCGCGGTAA-3) (Parada et al., 2016) and 806R (5-GGACTACNVGGGTWTCTAAT-3) (Apprill et al., 2015). Sequencing was performed on the Illumina MiSeq using a V2 2x250bp cycle standard flow cell
SUB14999624
Sc9Sep2920229IntsB