GSM9324340: Atrx-_1; Mus musculus; Hi-C
Hi-C on mNPCs was performed using the Genome-Wide Hi-C Kit (Arima Genomics) following manufacturer instructions. Briefly, 10 million cells were cross-linked using 1% formaldehyde for 10 mins and quenched with 0.125M glycine for 10 mins at room temperature. Fixed cells were permeabilized using lysis buffer and then digested with restriction enzyme cocktail. The resulting overhangs were biotinylated, followed by ligation and DNA purification. Proximity ligated DNA was sheared using Diagenode Bioruptor Plus and fragments were size selected from 200-600 bps using DNA purification beads (Ampure XP Beads). Libraries were made using Swift Bioscience Accel-NGS Plus DNA library Kit (Cat #21024) and sequenced using 2x150 base pair-end format on an Illumina NovaSeq6000 following the manufacturer protocol.
GEO
GSM9324340_r1