GSM9322963: Ocy454 cells, differentiation day 1, Rep 2; Mus musculus; RNA-Seq
Differentiated cells were lysed in RLT buffer. Lysates were passed through QIAshredder columns, then total RNA was isolated using the PureLink RNA Mini Kit with on-column DNase. FACS-sorted cells were pelleted and lysed in RLT buffer. Lysates were passed through QIAshredder columns, then total RNA was isolated using the Arcturus PicoPure RNA isolation kit with on-column DNase treatment. mRNA molecules were purified from total RNA using oligo(dT)-magnetic beads. After fragmentation and reverse transcription, adapters were ligated and products amplified by PCR. The double stranded PCR products were heat denatured and circularized by the splint oligo sequence. The ssCir DNA was formatted as the final library.
GEO
GSM9322963_r1