SARS-CoV-2 ARTIC3 human reference RNA, sDVR, and synthetic SARS-CoV-2 genome RNA
human reference RNA, synthetic deleted SARS-CoV-2 viral RNA (sDVR) and synthetic SARS-CoV-2 genome RNA (vgRNA) mixture were reverse-transcribed using random primers to synthesize cDNA, which was amplified via two multiplex PCR reactions with ARTIC v3 primers, following the ARTIC SARS-CoV-2 Library Prep Kit (Illumina) E7650 manual. Amplified DNA was prepared using the QIAGEN FX DNA Library Preparation Kit (QIAGEN) with fragmentation, and sequenced with paired-end 150bp reads on the Illumina NovaSeq X Plus platform.
SUB14897074
ARTIC3_sDVR_vgRNA_UHRR