RNA-seq of cucumbershoot
After passing the library check, different libraries are pooled according to the effective concentration and the target downstream data volume, and then sequenced to produce 161 bp paired-end reads. The basic principle of sequencing is Sequencing by Synthesis. Four types of fluorescently labelled dNTP, DNApolymerase and junction primers are added to the flow cell for amplification. When extending the complementary strand of each sequencing cluster, each fluorescently labelled dNTP added releases corresponding fluorescence, which is then captured by the sequencer, and converted into sequencing peaks by the computer software, thus obtaining information about the sequence of the fragment to be sequenced
SUB15453406
TR23792408