GSM9084975: THP-1 WT cells replicate 2; Homo sapiens; RNA-Seq
After harvesting PMA treated cells, cells were cultured in 12-well tissue culture plates in RPMI lacking FBS or A/A for 4 hours at 37C and 5% humidity. Cells were harvested from plates and dead cells were removed with a Miltenyi dead cell removal kit. Cells were then fixed for sequencing using the 10x Genomics FLEX protocol. Single cell suspension was sequenced on the Illumina NovaSeq X Plus platform.
GEO
GSM9084975_r1