Single-cell RNA-seq analysis of murine and human mesenchymal stem/stromal cells reveals macrophage-like phenotypic plasticity
Single-cell RNA-seq analysis of murine and human mesenchymal stem/stromal cells reveals macrophage-like phenotypic plasticity
Freshly isolated PαS cells were plated and cultured in either DMEM or RM for 1 week as described in the manuscript. After culture, cells were washed twice with PBS and dissociated into single cells. Approximately 10,000 viable cells per sample were prepared for loading onto the Chromium Controller (10x Genomics). Single-cell suspensions were directly processed according to the Chromium Single Cell 3′ Reagent Kits v3.1 workflow (10x Genomics), in which RNA capture and cDNA synthesis occur during single-cell encapsulation and barcoding within the Chromium system. Single-cell RNA-seq libraries were prepared using the Chromium Single Cell 3′ Reagent Kits v3.1 (Dual Index, 10x Genomics) according to the manufacturer's instructions (v3.1 Chemistry Dual Index).
European Bioinformatics Institute
E-MTAB-16511:Sample 1_p