GSM8553273: HG oligodendrocyte progenitors rep3; Mus musculus; RNA-Seq
RNA from cells were extracted from at least three biological replicates for each condition using TRIzol (Invitrogen) extraction and isopropanol precipitation. RNA samples were resuspended in nuclease-free H2O and further purified with RNeasy Mini Kit (Qiagen,  74106) with on-column DNase treatment. RNA purity was assessed by measuring the A260/A280 ratio using a NanoDrop. 100 ng of total RNA per sample was used for library construction using KAPA mRNA Hyperprep kit (Roche). The libraries were sequenced on an Illumina Novaseq X Plus platform according to the manufacturer's instructions. Depths of 15-30 million paired-end 150 bp reads were generated for each sample.
GEO
GSM8553273_r1