Honey
DNA was extracted from 15 g aliquots of honey following Laube et al. (2010) with minor modifications, including mechanical lysis with a TissueLyser II and purification with the DNeasy Blood & Tissue Kit (Qiagen). PCR inhibitors were removed (Zymo Research). A ~550 bp fragment of the chloroplast rbcL gene was amplified using primers rbcLa-F and rbcLa-R with Phusion High-Fidelity Master Mix (35 cycles, annealing 55 C). Extraction and PCR negative controls were included. Amplicons were checked on agarose gel and sequenced on an Illumina MiSeq platform (2300 bp, paired-end).
SUB16125610
H4_30