Lake Constance metatranscriptome study
For metatranscriptome sequencing, messenger RNA was enriched by depleting ribosomal RNA with the Ribo-off rRNA Depletion Kit (Bacteria; Vazyme, Nanjing, China)  to gain higher mRNA concentrations from the samples. The mRNA library was prepared with the TruSeq Stranded mRNA Library Prep (Illumina) and was sequenced on a NextSeq500 sequencer with the NextSeq 500/550 Mid Output Kit v2.5.
SUB15061946
WH20190425