Anaerobic digestion of spent coffee ground
10 samples were selected for microbiological community characterization. Their composition was analyzed by sequencing the 16S rRNA gene. The DNA was extracted following the protocols described in the FASTDNA SPIN kit for soil used, with verified quality and integrity. The 16S rRNA gene was amplified using the Ar915aF/Ar1386R and 341F/785F primer sets, and bioinformatics analysis was conducted by NGS Genomic Solutions (Piracicaba, SP, Brazil).
SUB15050131
Inoculum_bac