GSM9179214: Luminal-induced_Upk2 [BLA_7838_S4]; Mus musculus; RNA-Seq
For collection of 13-month Bas-induce and Lum-induce bladder tumor cells, the urothelium was separated from the muscle layer using tweezers, and tumor lesions were identified based on GFP signal under a fluorescent microscope and excised from the  epithelium with a scalpel before being transferred to a Collagenase/Hydrogenase digestion solution (F12/MEMD + 4.5%FBS + 10%C/H) to obtain single cell suspension. Library preparation was performed using 10x Genomics Chromium Single Cell 3' Solution with v3.1 chemistry following the manufacturer's protocol (10x Genomics). The library  purity, size, and quantity were validated by capillary electrophoresis using 2,100  Bioanalyzer (Agilent Technologies).
GEO
GSM9179214_r1