GSM9473501: WT KC-2; Mus musculus; RNA-Seq
Liver tissues enriched for metastatic B16F10 melanomas were harvested from WT or Vsig4-/- mice, cut into small pieces and digested with 0.5 mg/mL collagenase IV (Sigma) and 5 U/mL DNase I (Sigma) for 20 mins at 37℃ under shaking. The cell suspension was filtered through a 70 µm cell strainer and centrifugated at 400g for 5 mins at 4℃, followed by lysis of red blood cell inn ACK buffer (Biolegend).DAPI-CD45+Tim-4+ Kupffer cells  were sorted by FACS and further processed for bulk RNA-seq. RNA libraries for RNA-seq were prepared using VAHTS Universal V8 RNA-seq Library Prep kit  following manufacture's protocols.
GEO
GSM9473501_r1