ATAC-Seq of db/db mus musculus: adult male pancreatic islet
In brief, 50,000 cells were extracted from isolated mouse islets by TrypLE (Thermo Fisher, 12604013). Cells were lysed with lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2 and 0.1% (v/v) Igepal CA-630) at 4C for 10 min. Tagmentation and amplification were done following the manufacturers instruction with TruePrep DNA library Prep Kit V2 (Vazymes, TD501). Paired-end sequencing was performed by Annoroad Gene Technology (Beijing, China).
SUB15469166
db/db Rep1