RNA-Seq of mus musculus: male coronal forebrain section
The quality of RNA was determined using High Sensitivity DNA Kit reagents (Agilent, USA) and Agilent Bioanalyzer 2100 (Agilent, USA). 10 ul (i.e., 25%) of purified cDNA per sample was used as input material for Visium Spatial Gene Expression (10x Genomics, USA) library synthesis. The libraries were generated using the Visium Library Construction Kit following the manufacturers recommendations. Final library constructs of ~200-600 bp in length acquired from samples were subjected to sequencing on an Illumina system (NovaSeq 6000, S1 and SP flow cell, 200 mln read pairs per sample). The number of cycles was as follows: Read 1 - 28 cycles; i7 index - 10 cycles; i5 index - 10 cycles; Read 2 - 90 cycles.
SUB15522037
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