BTL_50cm_12C_rep3
For shotgun metagenomics, DNA was extracted with ZymoBIOMICS-96 MagBead DNA Kits (Zymo Research, Irvine, CA). Extracted genomic DNA was subjected to shotgun metagenomic sequencing. Illumina DNA Library Prep Kit (Illumina, San Diego, CA) with up to 500 ng DNA input following the manufacturers protocol using unique dual-index 10 bp barcodes with Nextera adapters (Illumina, San Diego, CA). All libraries were quantified with TapeStation (Agilent Technologies, Santa Clara, CA) and then pooled to equal abundance. The final pool was quantified using qPCR. The final library was sequenced on an Illumina NovaSeq X (Illumina, San Diego, CA) with 2x150 kit (300 cycles).
SUB15648528
BTL_50cm_12C_rep3