RECC CLIP-seq of Trypanosoma brucei : Trypanosoma brucei
(1)The cultured cells were crosslinked and then lysis.(2)RNA fragmentation with Rnase.(3)Immunoprecipitation of RBP-RNA complexes.(4)Dephosphorylation of RNA fragments and ligation of 3 RNA adapter.(5)Polyacrylamide gel electrophoresis and membrane transfer.(6)RNA isolation from membrane.(7)Dephosphorylation of RNA fragments and ligation of 3 RNA adapter.(8)Reverse transcription of RNA.(9)cDNA purification and adaptor ligation.(10)cDNA library amplication.
SUB14940674
X1_rep2