WT-miRNA-seq_rep1 (CRX1251940)
miRNA-seq: For each sample, total RNA was treated with DNase I for degrading DNA presenting in RNA samples. Ribosomal RNA was removed by using the Ribo-off rRNA Depletion Kit (Vazyme) and linear RNA was removed by RNase R (Epicentre). The small non-coding RNA was purified by using Agencourt RNA Clean XP magnetic beads. All steps were performed according to the manufacturer's protocols. There were two methods to analyze library's quality: check the distribution of the fragments size using the Agilent 2100 bioanalyzer (Life), and quantify the library using BMG (OMEGA). Finally, the qualified libraries were sequenced on the BGISEQ-500 (BGI-Shenzhen).
NGDC
CRX1251940
Sun Yat-sen University
DRX994883