Whole genome sequencing of Oryza sativa: leaf tissue of PB1  PBEF10 hybrid
Genomic DNA was extracted from young leaf tissue using the CTAB method. DNA quality and quantity were assessed using agarose gel electrophoresis and Qubit fluorometer. Paired-end sequencing libraries were prepared using the Illumina TruSeq DNA Library Preparation Kit following the manufacturers protocol. DNA was randomly fragmented, end-repaired, A-tailed, and ligated to Illumina adapters, followed by PCR enrichment and size selection (~350 bp insert size). Libraries were sequenced on the Illumina platform to generate paired-end reads.
SUB15720235
LIB_HYB_WGS