RNA-seq of Saccharum hybrid POJ2878: stem
This study was designed to obtain the transcriptomic profiles of the 9th internode of the main stem in Saccharum hybrid POJ2878. Total RNA was extracted from the 9th internode of the main stem collected from  a sugar cane grown at Shenzhen, China. RNA integrity was assessed using an Agilent Bioanalyzer, and samples with an RNA Integrity Number (RIN) > 8.0 were selected for library preparation. mRNA was enriched from total RNA using oligo(dT) magnetic beads. The purified mRNA was then fragmented and used as a template for first-strand cDNA synthesis with random hexamer primers, followed by second-strand synthesis. The resulting double-stranded cDNA was subjected to end-repair, A-tailing, and ligation with Illumina sequencing adapters. After PCR amplification, the final libraries were quantified and sequenced on an Illumina NovaSeq 6000 platform, generating 150 bp paired-end reads.
SUB15530546
POJ2878_RNA_seq_stem9