GSM8834376: Treg, sti-1, iBP SMART-seq, rep1; Mus musculus; OTHER
100,000 cells were collected directly into TRIzol, 40 ng total RNA was used for construction of sequencing libraries.  The reverse transcriptional reaction was initialized using oligo-dT30VN primer. RNA extraction and reverse transcriptional reaction were carried out by Annoroad Gene. Samples were PCR amplifie by SMARTer PCR cDNA Synthesis Kit, and PCR products were purified by Ampure XP beads. Library construction and sequencing were carried out by Annoroad Gene.
GEO
GSM8834376_r1