GSM8972036: EpA; Mus musculus; ATAC-seq
cold lysis buffer was used to get crude nuclei. 40,000 nuclei are subjected to a transposase reaction using Tn5 enzyme (Illumina). The transposed DNA fragments are recovered using the MinElute PCR Purification Kit (Qiagen).PCR amplification is carried out using 1X NEBNext High - Fidelity PCR Master Mix (New England Biolabs, MA). The amplified products are recovered using the MinElute PCR Purification Kit (Qiagen).
GEO
GSM8972036_r1