GSM9474008: TF_D14_WT_L; Mus musculus; RNA-Seq
Briefly, lungs were harvested from mock and influenza-infected WT and TFAM+/− mice (n=4 mice per group) and processed into single-cell suspensions. Viable cells were isolated and approximately 25k cells per sample were used for library preparation. Single-cell libraries were generated using Parse Biosciences split-pool combinatorial barcoding chemistry, which enables cell fixation, barcoding, and library construction through successive rounds of molecular indexing. Cell fixation, reverse transcription, barcoding, and library amplification were performed according to the Parse protocol. Barcoded libraries were pooled and sequenced on an Illumina platform to a depth of approximately 56,891 read per cell. Cells were fixed and processed for sequencing using the Evercode WT single-cell whole-transcriptome kit (Parse Biosciences, Seattle WA) according to the manufacturer's guidelines. Resulting nucleic acid sublibraries were evaluated for adequacy on an Agilent Bioanalyzer (RNA integrity number >8), pooled, and sequenced on an Illumina Novaseq 6000 sequencer using 100 base pair (bp) paired-end reads (Iowa Institute of Human Genetics, University of Iowa).
GEO
GSM9474008_r1