DoP_Sheep_Lung_401_Right_Cranial Full length 16S
The whole 16S gene was amplified using the 27f (AGRGTTYGATYMTGGCTCAG) and 1492r(RGYTACCTTGTTACGACTT3) primers with barcodes and adapters. Two nanogram of DNA was used as the PCR template for each sample. The PCR was run with Kapa HiFi HotStart DNA polymerase for 25 cycles. The subsequent PCR product of each reaction was cleaned up with Select-a-Size DNA Clean & Concentrator to retain fragment size greater than 300bp. To prepare for PacBio Sequencing, the pooled library was further processed using the SMRTbell prep kit 3.0 (Pacific Biosciences, Menlo Park, CA).
SUB16055796
DoP_Sheep_Lung_401_Right_CR_FL_16S