oself3-s-bulk-1 (CRX2114077)
The library was constructed using the NEXTFLEX Rapid DNA-Seq ( Bioo Scientific, USA ) kit. Firstly, an appropriate amount of DNA sample was taken, and DNA was randomly broken into small fragments by ultrasonic wave. After purification, End Repair Mix was added to fill it into a flat end, and then an A base was added to the 3 ' end to connect the Y-shaped connector ( see the instructions for end repair ) ; finally, after PCR enrichment ( included in the kit ), beads ( AMPure XP beads ) screened the target band of about 470 bp. After quantification by Qubit 4 fluorescence quantitative analyzer, the target band was denatured, and the single-strand cyclization reaction solution ( see the kit instructions for details ) was added for single-strand cyclization. The single-strand circular DNA molecule was replicated by a rolling ring to form a DNA nanosphere ( DNB ) containing more than 300 copies. The obtained DNBs were used.
NGDC
CRX2114077
Sichuan Agricultural University
DRX1009501