GSM9217716: iPS_Monocyte; Homo sapiens; RNA-Seq
iPS-monocytes, iPS-DCs, and iPS-tolDCs were collected in multiple batches, homogenized using ISOGEN II (Nippon Gene, Japan), and stored at -80℃. When the total cell count for each sample reached approximately 1×10⁶ cells, RNA was extracted using ISOGEN II (Nippon Gene) with Ethachinmate (Nippon Gene). The extracted RNA was further purified by removing contaminating DNA using the RNase-Free DNase Set (QIAGEN, Hilden, Germany). RNA sequence library preparation, sequencing, mapping, gene expression, and gene ontology (GO) enrichment analysis were performed by DNAFORM (Yokohama, Kanagawa, Japan). Furthermore, we analyzed the obtained data using the RNA-seq Analysis Portal (QIAGEN), including Ingenuity Pathway Analysis. Qualities of total RNA were assessed by Bioanalyzer (Agilent, Santa Clara, CA, USA) to ensure that RIN (RNA integrity number) is over 7.0. Library preparation was performed using the QIAseq UPX 3' Transcriptome Kit (QIAGEN, 333088) and MGIEasy Universal Library Conversion Kit (App-A) (MGI, 1000004155, Shenzhen, China) according to the manufacturer's instruction. RNA-seq libraries were sequenced using paired end reads (50nt of read1 and 25nt of read2) on a NextSeq 500 instrument (Illumina, San Diego, CA, USA).
GEO
GSM9217716_r1