RNA-Seq of biofilm on  PS incubated in RW
DNA was extracted from microplastics/glass using a DNeasy Power Water kit. 16S rRNA libraries were prepared with primers targeting the V4 region. Each template was amplified in triplicate, pooled, and cleaned using a Qiagen Qiaquick 96 Purification Kit. The samples underwent dual indexing PCR to ad adapters and indexes for runs on an Illumina MiSeq using paired-end (250) sequencing.
SUB14786314
ESF21MP-47