GSM8900244: Lambda_DNA_10pg_Zymo-Gold_CBS-seq.biol_rep2; Homo sapiens; Bisulfite-Seq
cfDNA was extracted and purified from pooled human plasma from Innovative Research Inc. using the Qiagen QIAamp Circulating Nucleic Acid kit. Two samples of ~250 ng of unmethylated Lambda genomic DNA (-dcm, -dam) from Thermo Fisher was fragmented with NEB UltraShear fragmentase for 25-30 minutes according to the NEB protocol. End repair, A-tailing, and ligation of a sequencing adapter were completed with the KAPA EvoPrep Kit. The NEB single molecule Methylated Adapter was used during ligation, and 3 μL of USER enzyme was added to the ligation reaction mixture following ligation and heated at 37 °C for 15 minutes for opening of the NEB Methylated Adapter loop. A final Cytiva beads purification with a 0.8x beads ratio was used to purify the adaptor ligated libraries. A bioanalyzer trace confirmed an appropriate library size of ~340 bp.
GEO
GSM8900244_r1