MiSeq biofilm sampling Artikutza (reservoir drawdown)
DNA was extracted with the Power Soil DNA Isolation kit (Qiagen). 16S marker (region v3-v4) for prokaryotes, the ITS2 for eukaryotes. Amplifications were performed with primers that contained Illumina overhang adapter sequences added to target specifics sequences. 341F/758R (Klindworth et al., 2013) primer sequences were considered for 16Sv3-v4 marker amplification and fITS7/ITS4rv (Ihrmark et al., 2012; White et al., 1990) for ITS2 amplification. In a second reaction, amplicons were dual indexed with Illumina Nextera XT index kit v2. After pooling libraries, the final library was sequenced on the Illumina MiSeq System with the MiSeq Reagent Kit v3 (paired-end 2x300bp) (UPV/EHU).
SUB15369168
S004_ITS2