Long read sequencing of Caenorhabditis elegans: reference strain
70 worms of each mutant line were placed onto five 10 cm NGM plates seeded with E. coli OP50 and allowed to lay eggs for 24 hours before removal. The offspring were grown until they reached the adult stage. High molecular weight DNA for this reference genome was extracted using Nanobind Nematode Big DNA Protocol (Circulomics) for Oxford NanoPore (ONT) sequencing. The DNA library was prepared with the ONT Ligation Sequencing Kit (SQK-LSK110) using instructions from the manufacturer (https://community.nanoporetech.com/protocols/genomic-dna-by-ligation-sqk-lsk110/checklist_example.pdf?device=minion). A total of 1.2 g was used to construct the DNA library in the flow cell. GridION sequencing was performed using a R9.4.1 SpotON flow cell (FLO-MIN106D), using the default script from the MinKNOW program, and files were obtained in the FASTQ format. Raw fastq reads were generated by the Guppy basecaller within MiniKNOW and were concatenated into one file.
SUB16245938
lofua