Metagenome or environmental sample from malaise trap - Site 337_M5
DNA from bugs collected in malaise traps was extracted with a DNeasy PowerSoil Pro Kit (QIAGEN) according to manufacturer's protocol with a final elution in 50ul molecular grade water. Three COI amplicons (BR5, MLJG and F230R) were amplified. First PCR used standard primers. Second PCR used standard primers with Illumina adapters. PCR products were checked on a gel, purified using MinElute DNA purification system, and quantified using a Qubit dsDNA BR assay kit. For each sample, equimolar amounds of each amplicon were pooled and tagged using Nextera XT Index Kit. Indexed samples were pooled, purified using magnetic bead purification, quantified using Qubit dsDNA BR assay kit, average length determined on Agilent Bioanalyzer 2100 before sequencing on an Illumina MiSeq using the V3 sequencing chemistry kit (2 x 300). 12% spike in of PhiX used as a control.
SUB14896937
M68