GSM9310437: Plasma microRNA-Seq (external ID 16.11.K2); Homo sapiens; miRNA-Seq
Peripheral venous blood was collected into EDTA tubes, centrifuged at 4 °C to separate plasma, and supernatant aliquots were stored at −80 °C. Total RNA enriched for miRNAs was isolated from plasma using Qiagen miRNeasy Serum/Plasma (CeGaT projects S7504, S8141) or miRNeasy Serum/Plasma Advanced kits (S10467) according to the manufacturer's protocol. Small RNA libraries were prepared from plasma-derived RNA using commercial microRNA library preparation kits. For CeGaT projects S7504 and S8141, the NEXTFlex Small RNA-Seq v3 kit (Bioo Scientific) was used with the maximum possible RNA input per sample (up to approximately 10.5 µl). For CeGaT project S10467, libraries were prepared with the QIAseq miRNA Library Kit (Qiagen) using up to 5 µl RNA input. Library preparation followed the manufacturers' protocols and included 3′ and 5′ adaptor ligation to small RNAs, reverse transcription, PCR amplification with indexed primers, and size selection to enrich fragments corresponding to adapter-ligated microRNAs. Final libraries were quantified, quality-controlled by fragment analysis and pooled equimolarly. Sequencing was performed on an Illumina NovaSeq 6000 platform with single-end reads (1 × 51 bp or 1 × 72 bp, depending on the sequencing run).
GEO
GSM9310437_r1