GSM9003753: WTY-Liver_M_VEH-2; Mus musculus; RNA-Seq
Total RNA was extracted from mouse liver tissue using the ISOLATE II RNA Mini Kit (Bioline, cat# BIO-52073) following the manufacturer's protocol. RNA concentration was measured using a NanoDrop spectrophotometer (Thermo Fisher Scientific) and RNA integrity was assessed using the Agilent TapeStation system. RNA-seq libraries were generated at the ERIBA Research Sequencing Facility (UMCG, Groningen, The Netherlands) using the Smart-3SEQ protocol (Foley et al., 2019) with 15 ng of input total RNA per sample. Libraries were pooled and sequenced on the Illumina NovaSeq X Plus PE150 platform (Novogene, Munich, Germany).
GEO
GSM9003753_r1