GSM9315334: MDA-MB-453 cells, DES 10μM + calcium 10mM, rep3; Homo sapiens; RNA-Seq
Total RNA was isolated from cell pellets using the TRIZOL reagent (Life Technologies). >2 μg total RNA was used for the construction of sequencing libraries. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library.
GEO
GSM9315334_r1