sequencing of bovine viral diarrhea virus from cattle
Viral RNA was extracted from purified viruses using TRIzolTM LS Reagent. The double-stranded complementary DNA was synthesised with modifications and sequenced using both Illumina and Oxford Nanopore Technologies. The ONT library was prepared using the rapid barcoding kit (SQK-RBK114-24). The samples were sequenced using a MinION Mk1B device and a SpotOn flow cell R10.4.1 version FLO-MIN114.
SUB15517591
ONT_28326