GSM8833904: GSC MES20, stiff, shIQGAP3; Homo sapiens; ChIP-Seq
GSC cells were cross-linked with 1% paraformaldehyde for 10 minutes, followed by quenching with glycine for 5 minutes at room temperature. After cell lysis, the fixed chromatin was sonicated to generate DNA fragments ranging from 200 to 600 bp. For library preparation, 1 to 10 ng of immunoprecipitated DNA and the NEBNext Ultra DNA Library Prep Kit for Illumina were used, and the samples were subjected to sequencing on the NovaSeq X Plus.
GEO
GSM8833904_r1