GSM9580120: MOLM-13 DMSO 72 h_3_1; Homo sapiens; RNA-Seq
RNA was harvested using NucleoSpin RNA, Mini kit from Macherey-Nagel and at least 900 ng per sample was used for library preparation RNA was sent to the University of Florida Interdisciplinary Center for Biotechnology Research Gene Expression and Genotyping Core (RRID: SCR_019145). RNA concentration was determined by Qubit and quality was determined using a Bioanalyzer RNA assay. Samples with a RIN score ≥ 8 were used for library preparation. Library preparation was done using the NEBNext Poly(A) mRNA isolation module (New England Biolabs) and NEBNext Ultra II Library prep Kit (New England Biolabs). Libraries were sequenced at 150 paired end reads at 25 million reads/sample using an Illumina Novaseq 6000 (Illumina)
GEO
GSM9580120_r1