GSM8767346: Root, Gansu, rep1; Angelica pubescens; RNA-Seq
The ground root powder is transferred to a centrifuge tube containing Trizol reagent and vortexed to mix well. The sample was placed at room temperature for 5 minutes to fully crack the sample. Add an equal volume of chloroform, mix well with vortex oscillation, and stand on ice for 5 minutes. Centrifuge at 4 ° C, 12000 rpm for 15 minutes, carefully drawing the upper water phase into a new centrifuge tube. The equal volume of isopropanol was added, vortexed and mixed, and then stood on the ice for 10 minutes. After centrifugation at 12000 rpm for 10 min at 4 °C, the supernatant was discarded and the RNA precipitate was obtained. RNA was washed twice with 75 % ethanol and centrifuged at 12000 rpm for 5 minutes at 4 ° C after each washing. Discard the supernatant and dry the RNA precipitate at room temperature ( pay attention not to over-dry ). The RNA solution was obtained by dissolving RNA with an appropriate amount of water without RNase. Total RNA extraction, mRNA enrichment and fragmentation, mRNA reverse transcription, end repair and A tail, ligation adapter, PCR enrichment, library quality control.
GEO
GSM8767346_r1