GSM8834070: siSMG6, Akron-seq, rep3; Homo sapiens; RNA-Seq
Total RNA was extracted using Trizol Reagent and treated with shrimp dsDNase (Thermo Scientific). 150 µg total RNA was used for two rounds of poly-A RNA selection were performed using the NEB Magentic mRNA Isolation Kit, followed by Akron5-seq library preparation (Ibrahim and Mourelatos, 2019).
GEO
GSM8834070_r1