GSM9693397: SHED_1_IgG; Homo sapiens; OTHER
Following CUT&Tag reaction, DNA was extracted using phenol-chloroform and ethanol precipitation. DNA fragments were purified and quantified for library preparation. CUT&Tag libraries were prepared using the Cleavage Under Targets and Tagmentation (CUT&Tag) method as described (Kaya-Okur et al., Nat Commun 2019). Libraries were constructed without the need for sonication or chromatin immunoprecipitation, using pG-Tn5 transposase to fragment and tag DNA adjacent to OSR2-bound loci.
GEO
GSM9693397_r1