Bacterial microbiome: Pinus taeda
Metagenomic DNA was extracted from 250 mg of soil using the DNeasy PowerSoil Kit (Qiagen, Germany). The V3V4 regions of the bacterial 16S rRNA gene were amplified using primers 515F and 806R adapted for Illumina sequencing. Three technical PCR replicates were performed per sample and pooled in equimolar concentrations prior to sequencing. Amplicon quality was assessed using a 2100 Bioanalyzer (Agilent Technologies) or Nanodrop, and quantified by qPCR using the KAPA NGS Quantification Kit (Roche).
SUB15958273
D1Gen4Rhizosphere