RNA-Seq ofVitis vinifera: Cab_Franc_1_20_1400_1
There were two 84-hour diurnal cycles (DC) that included temperatures fluctuating above and below 0 C (DC1: Jan 14 to Jan 17; DC2: Jan 24 to Jan 27). In DC1 and DC2, samples were collected 3 times/day, during the dark periods (6:00 and 18:00) and once in the light at warmest part of the day (14:00). The DC1 and DC2 periods bracketed four days (96 h) of constant daily temperatures below 0 C (Jan 18 to Jan 22). This constant below 0 C period was sampled in the light period only (14:00). Three replicate bud samples, each consisting of 3 buds per replicate were collected, each replicate from a separate vine for Reisling and Cabernet franc.  Bud samples were immediately plunged into liquid nitrogen and stored at -80 C until RNA extraction.
SUB16267493
CF_1_20_1400_1