RNA-seq of Zizania palustris root replicate 1 (CRX1854784; CRA027852)
Total RNA was extracted from each sample and assessed for purity, concentration, and integrity. Poly(A)+ mRNA was enriched using oligo(dT) magnetic beads and fragmented. First-strand cDNA was synthesized using random hexamer primers, followed by second-strand synthesis. Double-stranded cDNA was end-repaired, A-tailed, and ligated with sequencing adapters. After size selection and PCR enrichment, the libraries were sequenced with a paired-end 150 bp strategy.
NGDC
CRX1854784
Shanghai Normal University
DRX797529