GFP-2
Single-cell capturing and library constructions were carried out using Chromium Single Cell 3g Reagent kits v.2 and kit v.3 (10x Genomics) as well as Chromium Single Cell V(D)J Reagent kits (10xGenomics) according to the manufacturers protocol. In brief, single cell suspension, barcoded gel beads and partitioning oil were loaded onto Chromium Chip A/B to obtain single-cell gel bead-in-emulsion. And then the polyadenylated transcripts were reverse-transcribed in each gel bead-in-emulsion. Full-length cDNA along with cell barcode identifiers were amplified by PCR and sequencing libraries were prepared and normalized afterwards. The constructed library was sequenced on the Illumina platform.
SUB15322824
IL12