GSM9083374: Brain Cortical Endothelial cells from MCAO mice (Contralateral  side)-2; Mus musculus; RNA-Seq
The FACS sorted cells were suspended in solution as suggested by SMART-Seq v4 PLUS Kit User Manual (Takara Bio, Japan). The sorted cells were frozen on dry ice as soon as the sorting was done at The University of Texas Health Science Center in Houston (CPRIT RP180734). Libraries were prepared with SMART-Seq V4 PLUS Kit (R400752, Takara Bio, Japan) following the manufacturer's instructions. Agilent High Sensitive DNA Kit (#5067-4626) by Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, USA) was used to check the quality of the final libraries and the library concentrations were determined by qPCR using Collibri Library Quantification kit (#A38524500, Thermo Fisher Scientific, Texas, USA). The libraries were pooled evenly and went for the paired-end 150-cycle sequencing on an Illumina NovaSeq X plus System (Illumina, Inc., USA).
GEO
GSM9083374_r1