GSM8972985: ME_CnT_H3K4Me3_replicate3; Mus musculus; OTHER
Cells were harvested by Accutase dissociation and used in CUT&Tag (Kaya-Okur et al. 2020, PMID: 32913232) assays for different histone mark antibodies (H3K27ac, H3K4me1, H3K4me3, H3K27me3). Genomic DNA was extracted from cells after CUT&Tag using proteinase K digestion and Zymo DNA clean and concentrator clean-up. Genomic DNA isolated from each CUT&Tag cell sample replicate was subjected to end repair and 6-letter-seq library construction (Fullgrabe and Gosal et al. 2023, PMIID: 36747096).
GEO
GSM8972985_r1