GSM8553185: 15NA5; Mus musculus; RNA-Seq
Murine bone marrow-derived macrophages (BMDMs) and THP-1 human monocytic cells were cultured in RPMI-1640 complete medium. RNA-seq analysis of vehicle or HDAC6i, NextA treated BMDMs was performed as follows. FastQC (http://www. bioinformatics.bbsrc.ac.uk/projects/fastqc/) was used to evaluate the quality of the raw reads from M0, M1, and M2 samples in triplicates. The reverse and forward reads per sample were also assessed for paired-ended sequencing. Transcript quantification was performed with SALMON transcript quantification tool [47]. The reference genome served as the input index for the SALMON algorithm to report transcripts per mapped reads (TPM) per sample.
GEO
GSM8553185_r1