GSM8833432: in situ Replicate 2; Homo sapiens; OTHER
The CRISPR-Cas9 library used in this study was based on the human genome-scale GeCKO v2.0 library, which contains 123,411 sgRNA sequences targeting  targeting 19,050 human genes, with an average of 6 sgRNAs per gene. The library construction process was carried out using the proprietary CRISPR2 self-balancing library preparation technology and unbiased sgRNA fragment capture technology developed by Shanghai Genechem Co., LTD. The library construction involved the extraction of genomic DNA from tissue samples, followed by quality control assessments using UV absorbance spectroscopy, Qubit DNA quantification, and capillary electrophoresis. Subsequently, the genomic DNA was processed into libraries through PCR amplification and purification steps. The final libraries were sequenced using the Illumina HiSeq X platform.
GEO
GSM8833432_r1