GSM9114309: Intramuscular Adipose Tissue, Cow 2; Bos taurus; RNA-Seq
Fresh adipose tissue was dissected and minced on ice. Tissue was homogenized using a pre-chilled Dounce homogenizer in ice-cold lysis buffer. Nuclei were filtered through a 40 µm strainer, pelleted, washed, and resuspended for counting and quality assessment before loading onto the 10x Chromium platform. Single-nucleus RNA-seq libraries were generated using the 10x Genomics Chromium Single Cell 3' v3.1 kit, following the manufacturer's instructions. Libraries were sequenced on an Illumina NovaSeq 6000 platform.
GEO
GSM9114309_r1