Transcriptomic profiling of immature tomato fruits grown hydroponically with coral sand as the solid medium
Transcriptomic profiling of immature tomato fruits grown hydroponically with coral sand as the solid medium
Pericarp tissues were separated from fruits using a scalpel, and the samples were frozen in liquid nitrogen. Tomato seedlings were transplanted at the age of 4 w old and grown for 2 w hydroponically with different concentrations of nutrients. The phosphate concentration in the control solution (P100%) and low phosphate solution (P1%) were 100% and 1%, respectively. Samples were ground in liquid nitrogen into powder. Total RNA was isolated from the powdered samples using RNA iso plus (TaKaRa). One sample is from one fruit. Library was constructed as described in Lasy-seq protocol (https://sites.google.com/view/lasy-seq/)
European Bioinformatics Institute
E-MTAB-16425:15DAP_Coral_sand_5_s