DY86.A2.1 (CRX1892157; CRA028566)
DNA was extracted from sediment samples using the Qiagen PowerSoil Pro Kit according to the manufacturer's instructions (Qiagen, Hilden, Germany). DNA integrity was assessed through agarose gel electrophoresis, while its concentration and purity were determined using a Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and Qubit 4 Fluorometer (Thermo Scientific, USA). The V4 hypervariable regions of the 16S rRNA gene were amplified using a 30 microL PCR reaction volume. The PCR products were purified using magnetic bead purification and subsequently subjected to library preparation using a NEB  DNA PCR-free Library Prep Kit according to the manufacturer's protocols (New England Biolabs, Ipswich, MA, USA)
First Institute of Oceanography, Ministry of Natural Resources
DRX807943
NGDC
CRX1892157