GSM9216896: N8_3_CTRL_S15; Homo sapiens; RNA-Seq
bMPS were washed with ice-cold PBS twice, all supernatant removed, then snap frozen in liquid nitrogen. Samples were stored at -80°C before RNA extraction (Quick-RNA miniprep Kit, Zymo Research, Catalog # R1054). RNA quantity and purity were determined using a NanoDrop 2000c (ThermoFisher Scientific, Catalog # ND-2000C). Samples were processed by the Johns Hopkins Genomics Core for library preparation and Illumina sequencing. mRNA libraries were constructed by the core facility according to the manufacturer's recommended protocol with the NEBNext® RNA Library Prep Kit for Illumina Sequencing. Samples were then multiplexed and run on a NovaSeq 6000 instrument at Johns Hopkins Genomics Core Facility to generate 50 million, 150-base pair, paired-end reads per sample.
GEO
GSM9216896_r1