seq of microbes
Read Cleaning Process Description: Sequencing Strategy: Our sequencing was performed using the PE150 strategy, where a single read (Reads1) is sufficient to span the insert fragment. Therefore, only Reads1 was used in the analysis. Enzyme Recognition Site Identification: We scanned the Reads to extract sequences containing the enzyme recognition sites, which we term as EnzymeReads. Filtering Conditions for Clean Reads Generation: (1) Reads containing more than 8% N bases were filtered out and removed. (2) Low-quality Reads, where the number of bases with a quality value below Q30 exceeded 38% of the total number of bases in the Read, were also filtered out.
SUB14930280
T_C2309147