GSM8695824: FHs 74 Int cells,S100A12,4 hours,biol rep 3; Homo sapiens; RNA-Seq
RNA was extracted using the Rneasy Mini Kit (Qiagen) including DNase I digestion Sequencing libraries were prepared from total RNA samples at the Core Facility Genomics, Medical University of Vienna, with the QuantSeq FWD using the UDI V2 protocol (Lexogen). Eleven PCR cycles were performed for library preparation, determined by qPCR according to manual. Libraries underwent quality control on a Bioanalyzer 2100 (Agilent) with a High Sensitivity DNA Kit to confirm correct insert size and quantified using Qubit dsDNA HS Assay (Invitrogen). The pooled libraries were sequenced on a NextSeq500 instrument (Illumina) in 1x75bp single-end mode, generating approximately 3.8 million reads per sample.
GEO
GSM8695824_r1