GSM8899558: ATAC-seq of cortical neurons treated with PBS replicate 2; Mus musculus; ATAC-seq
Nuclei were extracted from primary cortical neurons following standard ATAC-seq procedures. Briefly, cells were lysed in a cold lysis buffer to isolate nuclei, which were subsequently used for Tn5 transposition reactions. ATAC-seq libraries were generated following the standard transposase-accessible chromatin protocol. Isolated nuclei were incubated with Tn5 transposase to fragment and tag accessible chromatin regions. After purification, transposed DNA was PCR amplified and size-selected. Libraries were sequenced on an Illumina NovaSeq 6000 platform using paired-end sequencing.
GEO
GSM8899558_r1