16S rRNA amplicon sequening of soil sample DNA
PCR amplicon libraries targeting the 16S rRNA encoding gene present in metagenomic DNA are produced using a barcoded primer set adapted for the Illumina HiSeq2000 and MiSeq. DNA sequence data is then generated using Illumina paired-end sequencing at the Environmental Sample Preparation and Sequencing Facility (ESPSF) at Argonne National Laboratory. Specifically, the V4 region of the 16S rRNA gene (515F-806R) is PCR amplified with region-specific primers that include sequencer adapter sequences used in the Illumina flow cell. The forward amplification primer also contains a twelve base barcode sequence that supports pooling of up to 2,167 different samples in each lane.
SUB15721470
Winchmore_exp2_79_S124_L001