GSM8766950: WT aneurysm tissue d7_1; Mus musculus; RNA-Seq
Aortas were homogenized in Trizol buffer and RNA was extracted using the RNeasy micro plus kit (Qiagen). RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dTTP. The cDNA samples were then fragmented, end-repaired, A-tailed, and ligated with Illumina adaptors. After size selection and PCR enrichment, the library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection.
GEO
GSM8766950_r1