GSM9001989: WT_testis; Mus musculus; RNA-Seq
testis samples were minced, and enzymatically digested using the MACS Tumor Dissociation Kit (Miltenyi) for 30 min with agitation. The dissociated cells were subsequently passed throughcell-strainer and centrifuged. The pelleted cells were then suspended in cell lysis buffer (Tiangen), and washed with 0.04% BSA in DPBS. Finally, the cell concentration was adjusted to 1,000 cells/μL(viability>80%) in all samples. Single-cell RNA-seq libraries were prepared with DNBelab C Series High-throughput Single-Cell RNA Library according to the manufacturer's instructions. Libraries were purified and quantified using the Qubit ssDNA kit (Thermo) and Qsep100 (Bioptic), and subsequently sequenced on DNBelab C4 /DNBelab TaiM4 Series Single-Cell Library Prep Set (MGI).
GEO
GSM9001989_r1