GSM8695029: W1,(NH4)2SO4,3d; Ganoderma lucidum; RNA-Seq
1. Leave the lysed sample or homogenate at room temperature for 5-10min to allow complete separation of the nucleoprotein from the nucleic acid. 2. Add 0.2ml of chloroform and shake vigorously for 15sec for 3min._x000D_ 2. Add 0.2 ml of chloroform, shake vigorously for 15 sec and leave at room temperature for 3 min. centrifuge at 12000 rpm at 4°C for 10 min._x000D_ 3. Aspirate the upper aqueous phase and transfer to a clean centrifuge tube, add an equal volume of isopropanol, mix well and leave at room temperature for 20min._x000D_  4. 12000rpm 4°C centrifuge for 10min._x000D_ 4. Centrifuge at 12000rpm for 10min at 4°C and discard the supernatant. 5. Add 1ml of 75% ethanol._x000D_ 5. Add 1ml of 75% ethanol to wash the precipitate. 12000rpm 4°C centrifugation for 3min, discard the supernatant. Dry at room temperature_x000D_ Dry at room temperature for 5-10min. 6._x000D_ 6. Add appropriate amount of RNase-free ddH2O to fully dissolve the RNA, and store the resulting RNA solution at -70°C or use immediately for subsequent experiments._x000D_ Store the obtained RNA solution at -70°C or use it immediately for subsequent experiments._x000D_ 7. Qubit2.0 was used to measure RNA concentration, and agarose gel was used to measure RNA integrity and genomic contamination._x000D_ _x000D_ Translated with DeepL.com (free version) Total RNA is subjected to mRNA isolation, fragmentation, double-stranded cDNA synthesis, cDNA fragmentation modification, magnetic bead purification and fragmentation sorting, and library amplification._x000D_ cDNA fragmentation, cDNA fragmentation modification, magnetic bead purification and fragmentation sorting, library amplification and other processes. After testing and quality control, the sequencing libraries can be applied to the Illumina platform._x000D_ The final result is a sequencing library that can be used on the Illumina platform after testing and quality control._x000D_ _x000D_ Translated with DeepL.com (free version)
GEO
GSM8695029_r1