moso_bamboo_Hic_2 (CRX1525603)
Hi-C libraries were prepared as described. Briefly, cells were fixed with formaldehyde to crosslink DNA-protein complexes, followed by cell lysis and chromatin digestion using a restriction enzyme. The resulting DNA fragments were labeled with biotin and ligated under dilute conditions to capture proximal DNA interactions. After ligation, biotin-labeled DNA fragments were purified and sheared to the desired size range. The Hi-C library was prepared by adding sequencing adapters, followed by amplification through PCR. Finally, the Hi-C library was sequenced using a 100 bp paired-end strategy.
International Centre for Bamboo and Rattan
DRX997361
NGDC
CRX1525603