GSM9834103: RAW264.7 cells, Negative Control, biol rep 3; Mus musculus; RNA-Seq
Total RNA was extracted using the TRIzol reagent (Invitrogen, CA, USA) according to the manufacturer's protocol. RNA purity and quantification were evaluated using the NanoDrop 2000 spectrophotometer (Thermo Scientific, USA). RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA).  Then the libraries were constructed by Hangzhou Astrocyte Technology Co., Ltd(Hangzhou, China). The libraries were sequenced on the illumina NovaSeq X Plus and 150 bp paired-end reads were generated. Sequencing libraries were prepared, and the quality of the libraries was assessed by Qubit (Thermo Fischer Scientific), 2100 Bioanalyzer (Aligent), and qPCR. Qualifying samples were sequenced by HiSeq (Illumina), and 150-bp paired-end reads were generated at a depth of 20×.
GEO
GSM9834103_r1