GSM8833813: H410_SCAT_primary_adip; Homo sapiens; RNA-Seq
Total RNA was extracted using Trizol reagent according to the manufacturer's instructions. The transcriptome of very low input (<10ng), degraded or high integrity samples was examined using Takara's SMARTer Stranded Total RNA-Seq Kit v2 as indicated by the manufacturer. RNA was fragmented according to the integrity of the sample, then reverse-transcribed (including a template-switching step) and PCR-amplified with primers bearing Illumina adapters and indexes. Next, cDNA originating from rRNA (18S, 28S as well as mitochondrial m12S and m16S) was depleted and the remaining molecules amplified through PCR. Libraries were quantified with Qubit dsDNA HS Assay Kit and their profile was examined using Agilent's HS D1000 ScreenTape Assay.
GEO
GSM8833813_r1