GSM9797675: kc167 cells,roX2 overexpression; Drosophila melanogaster; ATAC-seq
Nuclei were isolated as follows: cells were harvested and lysed in a cold lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl₂, 0.1% IGEPAL CA-630) for 10 minutes on ice. The nuclei were then pelleted by centrifugation at 500 × g for 10 minutes at 4°C. The cell pellet was resuspended in a transposase reaction mixture (25 µL of 2× TD buffer, 2.5 µL of Illumina transposase, and 22.5 µL of nuclease-free water) and incubated at 37°C for 30 min. Following transposition, the DNA was purified using a Qiagen MinElute kit. The library fragments were then amplified with 1× NEBnext PCR master mix and 1.25 µM custom Nextera PCR primers using the following thermocycling conditions: 72°C for 5 min; 98°C for 30 s; followed by 12 cycles of 98°C for 10 s, 63°C for 30 s, and 72°C for 1 min.
GEO
GSM9797675_r1