GSM9798690: ATAC_FOP2; Mus musculus; ATAC-seq
Nuclei from 1x10^5 MEFs were isolated using ATAC Lysis Buffer and tagmented using the Active Motif ATAC-Seq Kit at 37°C for 30 minutes. Purified DNA was PCR-amplified using Q5 High-Fidelity Polymerase (NEB) with 10 cycles of indexing, followed by SPRI bead size selection. Library quality was verified by Bioanalyzer (Agilent) and quantified using NEBNext Library Quant Kit. Libraries were sequenced as 150 bp paired-end reads on an Illumina platform (Genewiz).
GEO
GSM9798690_r1