GSM9178491: sample_60; Homo sapiens; Bisulfite-Seq
Genomic DNA from FFPE sample was bisulfite-converted using the EZ DNA Methylation-Gold Kit according to the manufacturer's instructions. Bisulfite-specific PCR (BSP) primers were designed using MethPrimer software. PCR amplification was performed with KAPA HiFi HotStart Uracil+ ReadyMix PCR Kit (Kapa Biosystems) for 35 cycles. BSP products from multiple genes were pooled equally, 5′-phosphorylated, 3′-dA-tailed, and ligated to barcoded adapters using T4 DNA ligase (New England Biolabs). Barcoded libraries from all samples were combined and sequenced on the Illumina platform
GEO
GSM9178491_r1