BM-kidney-WGBS_rep1 (CRX1251918)
WGBS-Seq: Sample tissues (200mg each) were broken up by trituration, and 1ug DNA extracted with lysis solution (Phenol: Chloroform: Isoamyl alcohol=25:24:1) was used to generate WGBS libraries by EZ DNA Methylation Gold Kit (ZYMO Research, Cat# D5006). Following steps were applied to generate WGBS libraries: DNA fragmentation, End repair; dA-Tailing; methylated adaptor ligation; selection of 350~500 bp fragments, bisulfite and PCR amplification (Skvortsova et al., 2019). For quality control, Qubit 3.0 Fluorometer (Life) was used for the quantitation of WBGS library, and Aglient 2100 Bioanalyzer (Agilent Techologies) was used for insert size testing of library. After that, the WGBS libraries were sequenced on an Illumina platform HiSeq X10 sequencers (Illumina) and 150 bp paired-end reads were generated.
NGDC
CRX1251918
Sun Yat-sen University
DRX994861