PacBio Iso-Seq reads of Vitis piasezkii DVIT2027 leaves
The first-strand synthesis and cDNA amplification were performed with the NEB Next Single Cell/Low Input cDNA Synthesis & Amplification Module (New England, MA, USA). The cDNA was then purified with ProNex magnetic beads (Promega, WI, USA) according to the Iso-Seq Express Template Preparation for Sequel and Sequel II Systems protocol (Pacific Biosciences, CA, USA). Long cDNA (> 2 kb) fragments were selected with ProNex magnetic beads (Promega, WI, USA), and at least 80 ng was used to prepare the cDNA SMRTbell library.
SUB15293509
19