RNA seq of circSETD5-si2
the purified RNA fragments were subjected to first strand and second strand cDNA synthesis following by adaptor ligation and enrichment with a low-cycle according to instructions of NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA). The purified library products were evaluated using the Agilent 2200 TapeStation and Qubit2.1(Life Technologies, USA) and then sequenced on HiSeq X Ten mode.
SUB14834912
20191027