scRNA of HCC in mus musculus: treated with PBS
Fresh tumor tissue from the livers treated with or without syringin was dissociated using1 mg/ml collagenase and 0.1 mg/ml DNase I with a gentle MACS Dissociator to generate a single-cell suspension. Cells were resuspended at a concentration of 1  106 cells/ml, and samples with viability exceeding 95% were used for library preparation. Library preparation followed the standard protocol provided by the 10X Genomics platform. Library quantification and quality assessment were performed using a DNA 1000 chip (Agilent Technologies), and samples were subsequently diluted and loaded onto the HiSeq 4000 (Illumina) for sequencing.
SUB15190973
PBS