GSM8798101: HCT116, sh-IGF2BP3-2; Homo sapiens; RNA-Seq
Total RNA was extracted from cell lysate samples using Trizol. The concentration and purity of the extracted RNA were assessed using a Nanodrop 2000 spectrophotometer. RNA integrity was evaluated by agarose gel electrophoresis, and the RNA Integrity Number (RIN) was determined using an Agilent 2100 Bioanalyzer. For library preparation, the RNA samples were required to meet the following criteria: a total RNA amount of at least 1 μg, a concentration of at least 35 ng/μL, an OD260/280 ratio of at least 1.8, and an OD260/230 ratio of at least 1.0. Library construction for the sequencing experiment was performed using the Illumina TruSeq™ RNA Sample Prep Kit method.
GEO
GSM8798101_r1