16S rDNA of mus musculus: adult skin male skin
Microbial genomic DNA was extracted using the MagPure Stool DNA KF Kit B (Magen Biotech), followed by PCR amplification of the 16S rRNA V3-V4 hypervariable regions with universal primers 338F (5'- ACTCCTACGGGAGGCAGCAG-3') and 806R (5'- GGACTACHVGGGTWTCTAAT-3'). Amplified libraries were purified using Agencourt AMPure XP beads (Beckman Coulter), quantified via Agilent 2100 Bioanalyzer (Agilent Technologies), and sequenced on the BGISEQ platform
SUB15306380
ABX-WD1-3-2