GSM9320847: HLCAN_rep3; Homo sapiens; RNA-Seq
Cell lines were seeded in 100 mm tissue culture dishes (Corning, NY, USA, Cat. 430167) at a density of 2.5x106 for HeLa, 3x106 for SiHa cells and 4x106 for HaCaT cells. Cells lines were incubated under previous conditions overnight. Subsequently, cells were treated with a 1:20,000 dilution of CEO or DMSO alone (vehicle control). After 4 hours of incubation, the cells were harvested for RNA extraction using the column-based extraction and purification system Zymo Quick-RNA MiniPrep (Zymo Research Corp., Irvine, CA, USA) following manufacturer's instructions. The obtained RNA was quantified by absorbance at 260/280 nm and stored at -80°C until further use. Total RNA was fragmented into 150 pb segmentes. Reverse transcription was achive to obtain cDNA follow by ligation of adapters at the ends.
GEO
GSM9320847_r1