TIAP_1
Sequencing libraries were constructed for each sample with index addition to distinguish sequences from different samples. Briefly, the V3 - V4 regions of the 16S rRNA gene were PCR - amplified using specific primers attached with barcodes. PCR products of appropriate sizes were selected via 2% agarose gel electrophoresis. Equal amounts of PCR products from each sample were pooled, followed by end repair, A - tailing, and ligation with Illumina adapters. The libraries were then sequenced on an Illumina paired - end sequencing platform to generate paired - end raw reads.
SUB16268850
LOHc1