GSM9324488: RiboSeq_runoff_GFP_nsp1; Homo sapiens; OTHER
RNA was harvested from ribosome pellets obtained from sucrose cushion sedimentation by TRIzol (Thermo Fisher Scientific) in combination with the Direct-zol RNA MicroPrep Kit (Zymo Research). RNA extraction, preadenylated linker ligation, rRNA depeletion, reverse transcription, cDNA circularization, PCR amplification
GEO
GSM9324488_r1