RNAseq of MALAT1 silencing experiments in three Patient derived lymphoma spheroids (PDLS) from Mantle Cell Lymphoma (MCL) patients
RNAseq of MALAT1 silencing experiments in three Patient derived lymphoma spheroids (PDLS) from Mantle Cell Lymphoma (MCL) patients
Peripheral blood was extracted and PBMC purified with Ficoll and cryopreserved in liquid nitrogen until the day of the experiment. Cells were grown with IL4, CD40L, HA-tag and BAFF as described in Araujo et al 2023 (PMID: 37031299) Silencing was performed using MALAT1 specific LNA-Gapmer (Qiagen) at 1micromolar vs Scrambled LNA-Gapmer (Qiagen) by gymnotic delivery along 5 days.  Total RNA was extracted using the RNeasy Plus Micro Kit (Qiagen), quantified with Qubit, and quality-checked using Tapestation 4200 (Agilent). Libraries were prepared from 10 ng of total RNA using the TAKARA_SMART-Seq® Total RNA Pico Input (ZapR® Mammalian) kit (following the manufacturer's instructions).
European Bioinformatics Institute
E-MTAB-16259:M3ALS_p