RNA-Seq of coral Pocillopora damicornis
RNA-seq transcriptome library was constructed using 1 g of total RNA and TruSeq RNA library prep Kit (Illumina). According to polyA selection method, messenger RNA was isolated by oligo (dT) beads and then fragmented by fragmentation buffer. Double stranded cDNA was synthesized using SuperScriptTM Double-Stranded cDNA synthesis Kit (Invitrogen, Carlsbad, California, USA). Finally, cDNA end-repair, phosphorylation, and A base were conducted. After 15 PCR amplification cycles, libraries were purified using 2% agarose to obtain the 300 bp cDNA fragments, and these paired-end RNA-seq libraries were sequenced using Illumina NovaSeq 6000 (2  150bp)
SUB15755405
T3BNT2