RNA-Seq of hepatopancreas: Penaeus vannamei
The mRNA was enriched using mRNA Capture Beads and fragmented at high temperatures. Using the fragmented mRNA as a template, the first strand of cDNA is created in a reverse transcriptase mixing system, and end repair and addition of A-tail are completed while the second strand of cDNA is synthesized. Next, the junctions were ligated and the target fragments were selected by purification using Hieff NGS DNA Selection Beads, followed by PCR library amplification and finally detection using Novaseq X Plus
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