GSM9835981: Sort-purified (live/CD8a+/GP33 tetramer+) GP33-specific CD8 T cells splenocytes from LCMV clone 13 infected mice, ATAC; Mus musculus; ATAC-seq
Cells were sorted from the pancreatic lymph node (IGRP-specific CD8 T cells) or from spleens of LCMV-clone 13 infected mice (GP33 specific CD8 T cells) into 0.01% BSA prior to freezing down in Bambanker media. Frozen cells were thawed and prior to nuclei extraction following Chromium Next GEM Single Cell Multiome ATAC + Gene Expression workflow. ATAC libraries were constructed using sample indexing PCR with 10x Genomics reagents, followed by dual-sided SPRI size selection. cDNA was amplified, cleaned, fragmented, end-repaired, A-tailed, and ligated using 10x Genomics reagents, with SPRI-based purifications throughout. Final gene expression libraries were indexed using 10x Genomics dual-index reagents and purified with SPRIselect.
GEO
GSM9835981_r1