GSM8728945: DNAme_256c_IN_Rep1; Xenopus laevis; MBD-Seq
250 pre-ZGA (4.5 hpf/256-cell) stage embryos were collected per replicate and nuclear isolation followed by genomic DNA extraction was performed as described in the manuscript.Methylated DNA was captured based on its affinity to the MethylCap (H6-GST-MBD fusion) protein according to the manufacturer's instructions (Diagenode; #C02020012). Methylated DNA and input DNA was purified and sequenced. Sequencing libraries were generated using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, #E7645S) as per the manufacturer's instructions using 6-8 PCR amplification cycles.
GEO
GSM8728945_r1