Thalassospira2_genome
Cells were grown in 45 mL and collected in late exponential growth. Cells were pelleted via centrifugation at 7,197 x g for 20 minutes at room temperature. The pellets were then extracted using a previously published standard phenol/chloroform procedure (Wilson 2001) with modifications as previously described (Coe et al. 2021). Genomic DNA was diluted and fragmented to 10-12 kb using a gTube (cat# 520079, Covaris) and cleaned with 0.45x SPRIselect beads (cat# B23317, Beckman). Indexed SMRTbell libraries were prepared following the Template Prep kit 3.0 protocol (cat# 102-141-700, PacBio), pooled, and subjected to MinElute cleanup.
SUB15663881
1907