GSM9265921: PDTF, Everolimus; Homo sapiens; RNA-Seq
PDTFs (n=192 per condition) were dissociated through enzymatic dissociation as described previously (Blondeel et al., Nat Protocols, 2025). The resulting filtered single cell suspensions were processed using the GEM-X Flex Sample Preparation v2 Kit (#PN-1000781, 10X Genomics) following manufacturer's instructions. Based on DAPI staining, cells were separated using the from debris by Fluorescence-Activated Cell Sorting (FACS) using the BD FACS symphony S6 and BD FACS symphony S8. Fixed cells were multiplexed prior to gel beads-in-emulsion (GEM) generation, barcoding, and library preparation according to the 10X Genomics User Guide (Document CG000787), using the Chromium GEM-X Flex Hybridization & Wash Kit (PN-1000789) and GEM-X FLEX GEM & Library Kit (PN-1000782, 10x Genomics).
GEO
GSM9265921_r1