Illumina NovaSeq 6000 paired end sequencing of SAMD01688614
Poly(A)+ mRNA was enriched from total RNA using magnetic beads and fragmented. First-strand cDNA synthesis was performed with random hexamer primers, followed by second-strand synthesis including dUTP incorporation and USER enzyme digestion to ensure strand specificity. Libraries were constructed with end repair, A-tailing, adapter ligation, and PCR amplification using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs).
University of Yamanashi
DRX731710