DNA-WGS
Use E Z.N.A  The total DNA was extracted by the bacteria DNA kit, and the database was built with DNA. After extraction, the DNA was broken by covaris M220 ultrasound, and then truseq was used   The nano DNA sample prep kit reagent is used for leveling, adding a at the 3 'end, and connecting the index connector. After this step, the library is enriched, and the cycles are amplified by PCR. Agarose gel is used to recover the target band in the library recovery. After recovery, tbs380 is used for quantitative analysis, and the data is mixed according to the proportion on the computer. After being on the computer, the bridge PCR amplification is carried out on the CBOT solid-phase carrier. Finally, it enters the second generation sequencing platform for sequencing
SUB15274534
dnb1