GSM8900394: Batch2, 40 day, young intact, central brain; Drosophila melanogaster; RNA-Seq
Central brains from either axotomized or intact flies were dissected at the age of 5 and 40 days and then placed into 1.5 mL RNase-free Eppendorf tubes, flash-frozen in liquid nitrogen, and then stored at -80°C. All dissections were performed between ZT0 and ZT4. Approximately 120-140 central brains were collected for each treatment and stored on dry ice. Tissue was homogenized and lysed with Triton X-100 in RNase-free water to isolate nuclei. The nuclei were then purified, centrifuged, resuspended in PBS with RNase Inhibitor, and then diluted to ~700 nuclei/µL. Next, standardized 10x capture and library preparation were performed using the 10x Genomics Chromium Next GEM 3' Single Cell Reagent kit v3.1 (10x Genomics; Pleasanton, CA).
GEO
GSM8900394_r1