Illumina HiSeq 2500 paired-end Sequencing of KAS20000444 (KAE20000060)
missing
We extracted total RNA from1-2 x 106tumor cells using TRIzol reagent Thermo Fisher Scientific, following the manufacturer's instructions, and treated DNaseI. We constructed RNA-seq libraries using the NEXTflex Rapid Directional RNA-Seq Kit Perkin Elmer, following the manufacturer's instructions. In brief, we selected and fragmented poly-A-tailed mRNA ligated double-stranded cDNA fragments with adapters and amplified them for 9 cycles of PCR.
KOBIC
KAE20000060
Pohang University of Science and Technology
DRX601970