GSM9474149: NOZ cells_B (Input); Homo sapiens; ChIP-Seq
NOZ cells were cross-linked with formaldehyde, and chromatin was extracted and sheared into fragments of 200-1000 bp. The DNA library construction procedure involves end repair and A-tailing of the immunoprecipitated DNA, followed by ligation of sequencing adapters; target-sized fragments are then purified using magnetic beads and amplified via PCR to generate the final sequencing library; the constructed library is quality-checked by agarose gel electrophoresis and quantified using Qubit 2.0 to confirm its concentration is suitable for sequencing; after passing quality control, different libraries are pooled based on their effective concentration and required data output, followed by sequencing on the Illumina HiSeq platform.
GEO
GSM9474149_r1