DNA_seq_from_AC1
All individuals used for sequencing originated from a single site in the Aconcagua River basin (32.50S, 70.58W, WGS 84) sampled in August 2021, where A. torrens were abundant and large-bodied. Genomic DNA was extracted from whole-body tissue using the E.Z.N.A. Insect DNA kit (Omega Bio-Tek), with protocol modifications to reduce the high protein and RNA content, involving extended incubation with the CTL buffer (2h at 37C instead of 30min at 60C) and prolonged RNAse treatment (1h at 37C instead of 10min at 70C). For short-read sequencing, DNA was extracted from a single individual, while long-read sequencing used pooled DNA from 14 individuals to reach required DNA amounts.Paired-end sequencing was performed using one lane of a NovaSeq 6000 S4 flow cell to produce approximately 110 Gb of data.
SUB15790807
AC1_LIB