GSM9217266: Sample 146, 12 months EGCG treatment (t12); Homo sapiens; RNA-Seq
Two 5 mL tubes of blood were collected in BD Vacutainer® K3E 15% Aprotinin 250KIU (SGSH;BD361017). Peripheral blood mononuclear cells were isolated by centrifugation in Blood Collection tube BD Vacutainer®, BD CPT™ (Avantor, BDAM362781). RNA was isolated using QIAshredder (Qiagen,79654) and RNAsy Mini-Kit (Qiagen, 74104) protocols. RNA libraries were prepared using the Illumina TruSeq Stranded mRNA Library Prep Kit following the manufacturer's instructions. Poly-A mRNA was purified using poly-T oligo-attached magnetic beads, fragmented, and converted to cDNA with random primers. Libraries were size-selected and sequenced on an Illumina HiSeq 2500 platform to generate 2×125 bp paired-end reads.