GSM9475405: Tcell_Control_Malate_3; Mus musculus; RNA-Seq
The CD8+ T cell pellets were harvested from the 1:5 T cell: tumor cell co-culture. The cells were then washed with Phosphate Buffer Saline (PBS). Three million cells were harvested and counted per replicate (n=3). RNA extraction was performed using the Aurum™ Total RNA Mini Kit (Bio-Rad). Libraries were prepared using the ABclonal mRNA-seq Library Prep Kit for Illumina (poly (A) selection). RNA integrity and quantification were confirmed on the AATI Fragment Analyzer and quantitative PCR. Quality assessment and sequencing were performed by Novogene Corporation, Sacramento. Sequencing was performed using Illumina NovaSeq X Plus (PE150).