GSM9068766: HUVEC, Dox washout (Input); Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 min. ChIP assay was performed using the SimpleChIP enzymatic chromatin IP kit (Cell Signaling Technology). Chromatin digestion was performed with micrococcal nuclease according to the manufacturer instructions. DNA library preparation and sequencing were performed by the Novogene Corporation. The DNA fragments were repaired, A-tailed and further ligated with Illumina adapter. The final DNA library was obtained by size selection and PCR amplification.