GSM9474832: PI 612713B Narrow-leaf TP3 replicate 2; Glycine max; RNA-Seq
Total RNA was extracted from shoot apical meristem tissue using TRIzol reagent followed by purification with RNeasy Mini Kit (Qiagen). RNA quality was assessed using Agilent 2100 Bioanalyzer (RIN > 8.0 required). RNA concentration was determined using Qubit fluorometer. Strand-specific RNA-seq libraries were prepared from polyA-selected mRNA using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina. Libraries were quantified using Qubit and quality-checked using Agilent 2100 Bioanalyzer before pooling for sequencing.