GSM9837173: Day 21 bleo NP-treated mouse 3; Mus musculus; RNA-Seq
Whole lung lobes were excised from large airway and trachea, and all 5 lobes were homogenized at 15000 rpm for 1-3 minutes in 1 mL TRIzol® reagent (Zymo Research) to achieve a pipetteable solution. This solution was spun to remove solids and 150 μL supernatant was diluted with 650 μL TRIzol®. Then, RNA was extracted using the Direct-Zol™ RNA MiniPrep Plus with Zymo-Spin™ IIICG Columns (Zymo Research Corporation R2070) according to manufacturer instructions. A TruSeq-based kit was used during library preparation. The adapter sequences for this kit are R1: AGATCGGAAGAGCACACGTCTGAACTCCAGTCA R2: AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT The product name of the library preparation kit is NEB. This pool was subjected to 151bp paired-end sequencing according to the manufacturer's protocol (Illumina NovaSeq 6000, Control Software Version: 1.8.1, RTA Version: v3.4.4).