GSM8640627: Infant 2 ctrl; Homo sapiens; RNA-Seq
Enteroids from three to four wells were pooled, resuspended in TRIzol, and lysed at room temperature for 15 minutes. RNA purification was performed with the Direct-zol RNA Microprep Kit (Zymo Research) using the manufacturer's protocol. A NanoDrop ND-2000 spectrophotometer was used for the quantification of isolated RNA. Sample quality control was performed using the Agilent 2100 Bioanalyzer. Qualifying samples were then prepped following the standard protocol for the NEBnext Ultra ii Stranded mRNA (New England Biolabs). Sequencing was performed on the Illumina NextSeq2000 with Paired End 59bp × 59bp reads