GSM8900576: Peripheral blood CD14+ monocyte, Healthy Control, Rep5; Homo sapiens; RNA-Seq
Cells were lysed in RLT buffer (Qiagen) containing 1% beta-mercaptoethanol. RNA was extracted using the PureLinkTM RNA Mini Kit (Invitrogen), as per manufacturer's instructions. Quality control was performed on the RNA samples using the high sensitivity RNA Analysis Kit (Agilent) on the 2100 Bioanalyzer Instrument (Agilent). RNA libraries were prepared for sequencing using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs) following the manufacturer's instructions. Quality control was performed on the RNA libraries using a high sensitivity DNA Chip (Agilent) on the 2100 Bioanalyzer Instrument (Agilent).