ONT long-read metagenome of agricultural soil: control, R4
Oxford Nanopore long-read shotgun metagenome sequencing was performed on DNA extracted (QIAGEN DNeasy PowerSoil Pro) from greenhouse pot microcosm soils under different crop treatments and no-plant controls (25 days), with libraries prepared using ONT Native Barcoding Kit 96 V14 (SQK-NBD114.96), pooled and sequenced on an ONT PromethION (PromethION 2 Solo), then basecalled and demultiplexed with Dorado (SUP).