16S rRNA gene sequence of Pseudomonas aeruginosa clinical isolate DW07 from pus sample, sequenced by Sanger metho
Amplicon sequencing of the 16S rRNA gene (~1048 bp) was performed from Pseudomonas aeruginosa isolate DW07 obtained from a diabetic foot ulcer pus sample. PCR was carried out using universal bacterial primers 27F and 1492R. The PCR product was purified and sequenced using the Sanger method on an ABI 3730xl DNA Analyzer. The assembled consensus sequence was 1048 bp in length