GSM8733172: Ctrl MO 256c+2hr Rep II.3; Xenopus laevis; RNA-Seq
Independent experiments were performed on two different frogs, serving as two biological replicates. Within each biological replicate, three sets of three embryos each were collected per time point, corresponding to three replicates within each independent experiment. Embryos were collected as described at hourly intervals after the pre-ZGA stage (i.e. 4.5hpf/256-cell stage): at 5.5hpf, 6.5hpf and 7.5hpf. Total RNA was isolated using the RNeasy kit (Qiagen, #74106) according to the manufacturer's instructions. Ribosomal RNA was depleted using custom-made oligomer mixes for Xenopus laevis rRNA as described in the manuscript. Sequencing libraries were generated using the NEBNext Ultra II Directional RNA Library Preparation Kit for Illumina (NEB, #E7760) as per the manufacturer's instructions using 12-13 PCR amplification cycles.