Shotgun DNA sequences present in soils cultivated with Coffea arabica
The genomic DNA was randomly sheared into shorter fragments. The obtained fragments were then end-repaired, A-tailed, and further ligated with Illumina adapters. The resulting fragments with adapters were size-selected and PCR-amplified, unless otherwise specified as PCR-free, before proceeding to purification. The experimental procedures for DNA library preparation are shown below.