Organ specific transcriptome profile of Bryophyllum pinnatum
RNA was isolated from each tissue with the Alexgen Total RNA kit (Aachen, Germany) and according to the manufacturer's protocol. Two replicates were present for each experiment. An Agilent tape station 4150 (Agilent Technologies, Palo Alto, CA, USA) was employed to verify the amount and quality of RNA. Ribosomal RNA was depleted using Human specific ribodepletion kit according to the user manual. Ribo-depleted RNA was fragmented, first & second-strand cDNA synthesized, end-repaired, 3 adenylated, adapter-ligated, selectively enriched for adapter-ligated DNA fragments by PCR amplification, followed by library validation on Agilent 4150 tape station (Agilent Technologies, Palo Alto, CA, USA). The final library was pooled with other samples, denatured & loaded onto the flow cell. Clustering and sequencing were carried out in a NovaSeq6000 System (Illumina, San Diego, CA) with a 2*150bp paired-end protocol.