Shotgun metagenomic sequencing of human gut metagenome: EF-2001 participant ELP072 Day 84 fecal sample
Genomic DNA was extracted from human fecal samples using the NucleoSpin 96 Stool Kit (Macherey-Nagel). DNA libraries were prepared using the Watchmaker DNA Library Prep Kit with the Twist Universal Adapter System. Genomic DNA was enzymatically fragmented, adapter-ligated, PCR-amplified, and purified using AMPure magnetic beads. Libraries were sequenced on an Illumina NovaSeq platform using paired-end 150-bp reads to generate whole-genome shotgun metagenomic data for characterization of the human gut microbiome.