GSM9035357: ChIP-Seq sample of 72-hour-old female embryos with anti-H4K20me3 antibodies; Bradysia coprophila; ChIP-Seq
Formaldehyde-fixed chromatin from approximately 20ml of 72-hour-old female embryos was used for each ChIP experiments. Anti-H3K9me3 antibodies (Active Motif, Cat.No. 39161), anti-H4K20me3 antibodies (Active Motif, Cat.No. 39671) and anti-H3K4me3 antibodies (Diagenode, Cat.No. C15410003) were used with the True MicroChIP & MicroPlex Library Preparation™ Package (Diagenode), according to manufacturer's recommendations. Formaldehyde-fixed chromatin was sheared using BioRuptor instrument (Diagenode). Three biological replicates were processed. DNA from precipitated material and input were used for library preparation using Illumina Nextera protocol. Illumina TruSeq Nano