ONT direct RNA sequencing of ATCC17978 trm W162* reconstructed mutant
Total RNA samples were prepared using the RNeasy Mini Kit (Qiagen, USA). RNA libraries for direct RNA sequencing were prepared using the Oxford Nanopore Technologies Direct RNA Sequencing protocol (DRS_9026_v1_revP_15Dec2016). Sequencing data were acquired with MinKNOW, base-called locally, filtered, mapped, and analyzed with EpiNano for per-site RNA modification features.