GSM9036132: Non lesion; Homo sapiens; RNA-Seq
Library was performed according to the manufacturer's instructions (10x Genomics, Chromium Next GEM Single Cell 5' v2 protocol). Briefly, single cells, reagents, and 5' gel beads containing barcoded oligonucleotides were encapsulated into nanoliter-scale Gel Beads-in-emulsion (GEMs). Within each GEM, cDNA was synthesized with a 5' gene expression library prep, which contains an Illumina R1 sequence, 10x Barcode, and Unique Molecular Identifier (UMI). Following GEM-RT, the barcoded cDNA was cleaned up with Silane DynaBeads, amplified by PCR, and the appropriate size fragments were selected with SPRIselect reagent for library construction. During the library construction, P5 and P7 sequences, along with a sample index, were added, resulting in a final 5' gene expression library.