GSM9085565: Sperm, RamF4_337_9, Treatment; Ovis aries; Bisulfite-Seq
Genomic DNA was extracted from sperm samples using the Quick-DNA Miniprep Plus (Zymo Research, CA, US). DNA samples were bisulfite-treated with the Zymo EZ DNA Methylation-Lightning kit, followed by library preparation using Illumina universal adapter sequences and Pico Methyl-Seq Library Prep Kit (Zymo Research).