Pimelodus grosskopfii
DNA was extracted from ethanol-preserved tissues by using the GeneJET Genomic DNA Purification Kit (Thermo Scientific). DNA quality was evaluated by electrophoresis on 0.8% agarose gels and EZ-vision staining (AMRESCO). DNA was quantified with a NanoDrop 2000 spectrophotometer (Thermo Scientific) and diluted to a final concentration ranging from 30 to 50 ng/uL. At least 0.5 g of high-molecular-weight DNA was employed to prepare RAD libraries using a 6-bp restriction enzyme (PstI) for digestion, followed by sonication. Finally, a single-end sequencing step was conducted by the National Center of Genomic Sequencing (Colombia) in collaboration with Floragenex Inc (USA) (https://floragenex.com) on one lane of an Illumina HiSeq2000machine.