GSM8490579: CSR_SAPPKHL-1; Mus musculus; OTHER
Genomic DNA was extracted from treated B cells on day 3 for SJ HTGTS analysis and day 4 for CSR analysis. For SJ analysis, sonicated DNA was linearly amplified with primer (/5Bios/GGCAGCCTACCAAGAACAAC) and nested amplified with primer (5′-CGGTGGTACCTCACCCTTA-3′). Germline sequence was removed with EcoRI (NEB) digestion. For CSR analysis, libraries were prepared according to the protocol in (Hu J, et al. Nat Protoc 11:853–871 (2016)). Linear amplifications were performed using Sμ-specific biotin primer (/5Bios/CAGACCTGGGAATGTATGGT) and followed by nesting PCR with (5′-CACACAAAGACTCTGGACCTC-3′). AflII was used to remove germline sequence. high-throughput genome translocation sequencing (HTGTS). Sequenced with MiSeq 500v