GSM8697653: P14 CD8+ T cells sgBcor, Day14 post infection; Mus musculus; ATAC-seq
Control, BCOR-deficient, ZC3H12A-deficient and BCOR/ZC3H12A-deficient P14 cells were transferred into Cas9+ B6 mice that were infected with LCMV Clone 13 one day before cell transfer. Fourteen days after transfer, Thy1.1+ P14 cells from spleen were sorted by a S3e cell sorter with purity > 98% and sent to Novogene for scATAC-seq. scATAC-seq libraries were generated using the 10x Genomics Chromium Cell ATAC Reagent Kit (v1). In brief, sorted CD8+ P14 T cells were washed with 0.04% BSA PBS, then approximately 40,000 cells were subjected to the nuclei preparation protocol according to the manufacturer's instructions. Then, 16,000 nuclei were loaded into a 10x Chromium controller. All downstream library preparation steps were performed according to the manufacturer's instructions. Libraries were sequenced on an Illumina NovaSeq.