Oryza sativa RNA sample: MM112-1
Total RNA was extracted with the TRIzol kit (Thermo Scientific, USA) following the manufacturers protocols. RNA quality was determined by Nanodrop 2000, agarose electrophoresis and Agilent 5300, and qualified RNA was reserved for library construction. mRNA was enriched by Oligo (dT) beads and fragmented. First-strand cDNA was reverse-transcribed with random hexamers, and second-strand cDNA was then synthesized. Purified cDNA fragments were end-repaired, poly(A)-tailed and ligated to Illumina adapters. Fragments of appropriate size were recovered by agarose gel electrophoresis and amplified by PCR. Libraries were sequenced on an Illumina HiSeq 2000 platform. Differentially expressed genes (DEGs) were identified using edgeR.