WGS of Flavobacterium covae
A single large (3-4mm diameter) pure colony was directly excised from the agar and extracted using a Quick-DNA Miniprep Plus Kit (Zymo Research Group; Cat. No. D4068). Oxford Nanopore Technology (ONT) long reads were generated using a Minion Mk1C using the Rapid Barcoding 24 Kit V14 (SQK-RB114.24) on a R10.4.1 flow cell (FLO-MIN114). POD5 files were basecalled in MinKNOW v.24.02.8 using Dorado super accuracy basecaller model (SUP; dna_r10.4.1_e8.2_400bps_5khz_sup) enabling barcode and adapter trimming at basecalling.