GSM8736123: H460, control, rep 2; Homo sapiens; RNA-Seq
RNA was isolated according to manufacturer's protocol (NucleoSpin® RNA II (MACHEREY-NAGEL GmbH & Co. KG, Düren, 244 Germany)). Concentration and quality of RNA was measured with Qubit (Invitrogen, Waltham, MA, 245 USA) and Agilent Bioanalyzer DNA HS (Agilent, Santa Clara, CA USA) Libraries were construced with QuantSeq 3' mRNA-Seq Library Prep Kit FWD