GSM9475313: MH-S cells, treated with SiO2 microrods, sample 1; Mus musculus; RNA-Seq
RNA was isolated using Direct-zol™ RNA Microprep Kit (Zymo-Research, #R2060) following manufactor instructions. 100 ng of RNA was used for construction of sequencing libraries. Libraries were constructed with the Smartseq2 protocol. Poly-A RNA was enriched with oligo dT primer and reverse transcribed. Resulting cDNA was pre-amplified and purfied by magnetic beads, before 4ng cDNA was tagmentated. Tagmentated DNA was barcoded, PCR amplified and purfied, yielding sequencing libraries.