GSM9116817: spr-5; met-2_control RNAi_Rep2; Caenorhabditis elegans; RNA-Seq
Total RNA was isolated using TRIzol reagent (Invitrogen) from 500-1000 starved L1 larvae born at 20°C overnight on unseeded NGM plates. Total RNA was sent to Georgia Genomics and Bioinformatics Core (University of Georgia, Athens, Georgia) for standard Poly-A RNA-seq services (Illumina Nextseq, 50bp paired-end reads) Libraries were prepared by HAIB-GSL (for HiSeq).