GSM9068779: 5mC mapping in HepG2 cells without DEHP exposure; Homo sapiens; Bisulfite-Seq
Genomic DNA of HepG2 cells was extracted using Tissue DNA kit (Omega Bio-Tek Inc., Norcross, GA, USA) Briefly, the isolated genomic DNA from theHepG2 cells with or without DEHP exposure was fragmented into 200-400 bp using a Covaris S220 Focused-ultrasonicator (Covaris, USA). The Accel-NGS Methyl-Seq DNA Library kit (Swift, USA) was utilized to construct the sequencing library. Library quality was assessed using the Agilent 5400 system (Agilent, USA). Paired-end sequencing of sample was performed on Illumina platform (Illumina, USA).