D1 (CRX832912; CRA013103)
The mRNA of the quality-checked total RNA was initially isolated and enriched utilizing oligo(dT) beads. The mRNA was then fragmented to 200 bp using relevant reagents. Subsequently, the first strand of cDNA corresponding to mRNA was synthesized with random primers, while the second strand was generated using buffer, dNTPs, RNase, and DNA polymerase. The purified double-stranded cDNA was recovered using the corresponding kits.