GSM9084114: Sample13-HRS; Homo sapiens; RNA-Seq
RNA was extracted using the Arcturus PicoPure RNA Isolation kit (KIT0204, Life Technologies, Carlsbad, CA) with RNAse-Free DNase (79254, Qiagen, Venlo, Netherlands) to isolate high quality RNA from viable flow-sorted cells. We applied the SMARTer Ultra Low Input RNA Kit (Clontech, Mountain View, CA) to 1-4 ng of input total mRNA.  Illumina-compatible libraries were constructed from cDNA using KAPA LTP Library Preparation Kit (KK8221, Woburn, MA).