Single-cell RNA sequencing of bone marrow HSPCs isolated from patients after myocardial infarction and control patients
Single-cell RNA sequencing of bone marrow HSPCs isolated from patients after myocardial infarction and control patients
Human bone marrow was isolated from the sternum during surgery from patients who had experienced a myocardial infarction, as well as from control patients. The samples were subjected to Ficoll separation and subsequently frozen. On the day of sorting, the samples were thawed, and a total of 7,000 to 10,000 human hematopoietic stem and progenitor cells (HSPCs) (Lineage negative, CD38 negative, CD34 positive) were sorted in 2% BSA in PBS in two biological replicates. n/a The quality of the obtained cDNA and final libraries was assessed by capillary electrophoresis (Fragment analyzer, HS NGS Fragment Kit, Agilent).