GSM8697615: combination treatment; Mus musculus; RNA-Seq
Mice were sacrificed and ascites was collected, depleted for erytrocytes, filtered and cryopreserved until scRNA-seq was performed. Cells of animals of the same treatment condition were pooled. If applicable, dead cells were removed using the dead cellremoval kit (Miltenyi Biotec). Single cells were captured using the BD Rhapsody HT Express and the BD Rhapsody Scanner. cDNA synthesis and WTA library constructions was perfomed according to the manufacturer's instructions.