Exome-seq of PaTu-8988T KRAS knockout clone 275
Genomic DNA was extracted from KRAS KO and intact clones and the parental cell line using the High Pure PCR Template Prep Kit (Roche, 11796828001). Whole-exome sequencing libraries were prepared by the Yale Center for Genome Analysis (YCGA) using the IDT xGen V2 Exome Enrichment Kit and sequenced on the Illumina HiSeq platform (paired-end, 100-bp reads) to a mean target coverage of approximately 30.