GSM9067379: Sh-2-1; Mus musculus; RNA-Seq
Advanced molecular biology equipment is employed to test the purity, concentration, and integrity of the extracted total RNA:(1) NanoDrop 2000 Spectrophotometer: used to determine/measure the purity and concentration of RNA;(2) Agilent 2100 Bioanalyzer / LabChip GX system: used to accurately assess/measure the integrity of RNA. After the sample passes the testing phase, library construction is performed according to the following key procedures: (1) Enrich eukaryotic mRNA using magnetic beads coated with Oligo(dT); (2) Add Fragmentation Buffer to randomly fragment the mRNA; (3) Use mRNA as template to synthesize the first cDNA strand and the second strand, followed by cDNA purification; (4) Subject the purified double-stranded cDNA to end repair, A-tailing, and sequencing adapter ligation, then perform size selection using AMPure XP beads; (5) Finally, obtain the cDNA library through PCR amplification.