DiMeLo-seq of PD-NC4 CENPA:clone C1
DiMeLo-seq was performed for CENP-A (ADI-KAM-CC006-E, Enzo, 1:50) or H3K9me3 (ab8898, Abcam, 1:50) with 200 nM mouse Hia5 nanobody or 200 nM pA-Hia5.  After Hia5 activation, modified UHMW DNA was extracted using the NEB Monarch UHMW DNA Extraction Kit. Library preparation was performed with the Nanopore Ultra-Long DNA Sequencing Kit (SQK-ULK001).