GSM8730539: spy4_secRNAi, seedling, DEX, rep1; Arabidopsis thaliana; RNA-Seq
RNA was extracted using the Qiagen RNeasy Plant Mini Kit (Hilden, Germany). Harvested tissues collected above for  WT, spy-4, shSEC RNAi spy-4 (Dex and mock treated) and acinus pnn (n=4) were ground in 2mL tubes with liquid nitrogen. For each sample, 30μl RNase free water was used and incubated on the column for 5 minutes prior to elution. The eluate was run through the column a second time to maximize concentration. Libraries were generated by Novogene using poly A enrichment (UofC, California)