GSM8768664: OC_40; Homo sapiens; RNA-Seq
Total RNA was extracted from FFPE samples with RNeasy FFPE Kit (Qiagen) following the manufacturer's protocol. RNA concentration was measured fluorimetrically using Qubit 4 (Thermo Fisher Scientific). The RNA integrity was accessed by Tapestation 4150 (Agilent). For depletion of ribosomal RNA and library preparation, the KAPA RNA HyperPrep Kit with RiboErase (HMR) (Roche) was used. KAPA single-indexed adaptors (Roche) or KAPA unique dual indexed primers (Roche) were used for multiplexing samples in one sequencing run. Library concentrations and quality were measured with the Qubit 4 (Thermo Fisher Scientific) and Tapestation 4150 (Agilent).