Sequencing of strawberry infecting Colletotrichum isolates
Mycelium from single-spore culture was collected and DNA was extracted using a DNeasy Plant Mini Kit (QIAGEN). Libraries were prepared using the Rapid sequencing DNA V14 - barcoding (SQK-RBK114.24; ONT) kit according to the manufactures instructions. The libraries were loaded on R10.4.1 flow cells (FLO-114; ONT) and sequenced for 48 hours on a MinION Mk1D. Base calling and trimming was performed using GUPPY v6.5.7.