Wheat straw compost samples at different stages
In this study, WS and WSI samples were chosen for DNA extraction. The 16S rRNA gene containing the V3 and V4 regions was amplified by PCR using universal primers 341F (CCTAYGGGRBGCASCAG) and 806R (GGACTACNNGGGTATCTAAT). High-throughput sequencing of the 16S rRNA gene from the aforementioned samples was ultimately carried out using the Illumina HiSeq platform at Novogene (Beijing, China).