ATAC-seq of si-SMARCA5-3
Nuclei suspensions are incubated in a Transposition Mix that includes a Transposase. The Transposase enters the nuclei and preferentially fragments the DNA in open regions of the chromatin. Simultaneously, adapter sequences are added to the ends of the DNA fragments. Incubate the transposition reaction at 37C for 30 min. Immediately following transposition, the products are purified using a QIAGEN minielute kit, amplified as described in, and sequenced using Illumina NovaSeq 6000 by Gene Denovo Biotechnology Co. (Guangzhou, China).