Cold dessert India
The Qubit quantified metagenomics DNA of ~ 0.025 g of DNA was used as a 16S amplicon library preparation template. The 16S rRNA V3-V4 hypervariable region was amplified using specific V3-F and V4-R universal primers with an overhang adapter via a polymerase chain reaction. Afterwards, Illumina Miseq, with 2x300PE v3 sequencing kit, was used to produce the sequence data