ONT resequncing of a lrPCR product of MnA-1 LSC01-21
Viral DNA was amplified via long-rang PCR (TaKaRa LA PCR Kit Ver.2.1; Takara Bio Incorporation). Primers were MnA-1F_2600 (TTGTCACTCAACCAGATCCGG) and MnA-1R_2600 (GTCGTTCTTTTGTGCGCACT).  PCR product was purified using a QIAquick PCR Purification Kit and the purified amplicon concentration was normalized to 15uM with nuclease free water and shipped to Plasmidsaurus (Eugene, OR, USA) for ONT sequencing.