RNA sequencing analyses of human liver cancer cell line HepG2 treated with control shRNA and YTHDC1 knockdown shRNA
RNA sequencing analyses of human liver cancer cell line HepG2 treated with control shRNA and YTHDC1 knockdown shRNA
HepG2 cells were cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum. The cells were maintained at 37 °C in a 5% CO2 humidified atmosphere. When the cell density reached approximately 80%, the cells were collected. 5x10^6 HepG2 cells were used in shCtrl and YTHDC1 knockdown group. Total RNA was isolated by Trizol (Invitrogen) according to the manufacturer's protocols. mRNA was purified by NEBNext Poly(A) mRNA Magnetic Isolation Beads (NEB, USA). The RNA libraries were constructed using the NEBNext Ultra Directional RNA Library PrepKit (NEB, USA).