GSM9837733: RAW 264.7 Cells, Control, 24h, rep2; Mus musculus; RNA-Seq
Total RNA was extracted from RAW264.7 cells. RNA integrity was assessed using a Qsep100 system, and RNA concentration was measured using a NanoDrop spectrophotometer. RNA samples that passed quality control were used for library construction. RNA-seq libraries were prepared using an oligo(dT)-based mRNA-seq library construction protocol. Briefly, mRNA was enriched from total RNA, fragmented, reverse-transcribed into cDNA, end-repaired, A-tailed, adapter-ligated, PCR-amplified, and purified with magnetic beads. Library quality was assessed using Qubit and an Agilent Fragment Analyzer. Qualified libraries were sequenced on an Illumina NovaSeq 6000 platform to generate paired-end 150 bp reads.