Spatial transcriptomics of bat gut sections
Spatial transcriptomics of bat gut sections
Gut sections were frozen in OCT according to 10X Genomics Visium protocol. Then 10x Genomics Visium protocol was applied on the (OCT)-embedded fresh frozen samples. All tissues were sectioned using a Leica CX3050S cryostat and were cut to obtain a 10 µm width.  Samples were then tested for RNA integrity using a Tapestation 4200. Only blocks with RIN above 7 were used. Tissue optimization was performed, resulting in a permeabilization time of 23 minutes The Visium spatial gene expression protocol from 10X Genomics was applied by loading a total of 11 gut sections from two individual bats onto the Library Preparation Slide (4 grid sections) and following the manufacturer's protocol cDNA library preparation was were carried out according to the manufacturer's protocol.