RNA-seq from Wound phloem of Austrian pine stem that was sampled after 1 dpi
RNA extraction was carried out following a standard CTAB protocol (Chang et al., 1993) modified for pine samples (extraction in 500 l buffer preheated at 65 C for one hour), from three biological replicates of each treatment combination. RNA extracts were then treated with amplification-grade DNaseI per manufacturers instructions (Invitrogen, ThermoFisher Scientific, Waltham, MA, USA) and then treated with the RNeasy Plant Mini Kit per manufacturers instructions (Qiagen, Germantown, MD, USA). Samples were quantified spectrophotometrically using a NanoDrop (Thermo Scientific, Wilmington, Delaware, USA) and sent to the Yale Center for Genome Analysis (YCGA, New Haven, CT, USA) for quality control assessment and sequencing. The concentration and purity of RNA were further assessed using a Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA, USA). The cDNA libraries were subsequently generated by the ribodepletion method using TruSeq Stranded Total RNA with Ribo-Zero Plant (Illumina, San Diego, CA, USA) and subjected to 150-bp paired-end sequencing on an Illumina NovaSeq 6000 (Illumina, San Diego, CA, USA), with a targeted depth of 30 million reads per sample.