RNA-seq of Bacillus:SL
Total RNA was extracted using Total RNA Extractor (Trizol) (Sangon, China), and RNA concentration was measured with Qubit 2.0. RNA integrity and genomic contamination were assessed using agarose gel electrophoresis. Qualified RNA samples were stored at -80C. The total RNA quantity was confirmed with a Qubit RNA Assay Kit (Life, China). rRNA was removed using the Ribo-off rRNA Depletion Kit (bacteria) (Vazyme, China). The VAHTS Stranded mRNA-seq Library Prep Kit for Illumina (Vazyme, China) was used to fragment RNA and synthesize double-stranded cDNA, followed by end-repair and dA-tailing for adapter ligation. PCR amplification and purification were performed using VAHTS DNA Clean Beads (Vazyme, China), and the library quality was checked with 8% PAGE gel electrophoresis. Purified cDNA was quantified with a Qubit DNA Assay Kit.