GSM9069581: THP-1 cells, treated by FL-Fc-DM1 (1 nM); Homo sapiens; RNA-Seq
Cells from each group were extracted by using the Trizol method. RNA quality was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies, CA, USA) and checked using RNase-free agarose gel electrophoresis. Total mRNA was enriched by Oligo(dT) beads. Then, the enriched mRNA was fragmented and reverse-transcripted into cDNA with random primers. Second-strand cDNA was synthesized by DNA polymerase I. The cDNA fragments were purified with the QiaQuick PCR extraction kit (Qiagen, Venlo, The Netherlands), end repaired, poly(A) added, and ligated to Illumina sequencing adapters. The ligation products were size-selected by agarose gel electrophoresis, PCR amplified and sequenced using Illumina HiSeq2500 by Gene Denovo Biotechnology Co. (China).