GSM8608237: L215-B-CD4_VDJ; Homo sapiens; OTHER
Blood diluted with PBS-EDTA and 2% Ficoll-Paque PLUS, incubated in 37C for 5 minutes and centerifuged at 300xg for 5 minutes with supernatant discarded. Tregs and CD4+ T cells sorted ina BD FACS Aria III. Single-cell mRNA, dual-indexed, sequencing libraries were generated following the Chromium Single Cell 5' v2 protocol with Feature Barcode technology for Cell Surface Protein and Immune Receptor Mapping For each 10x run, patient samples were hash-tagged and pooled, using one antibody-derived tag per patient following the CITE-Seq protocol (Biolegend) and then flow-sorted to generate separate compartments of CD4+ T-cells and FOXP3+ T-cells before proceeding with the 10x assay.