Bulk RNA-sequencing of testes and kidneys from wildtype and Gfi1[R412X] embryonic mice
Bulk RNA-sequencing of testes and kidneys from wildtype and Gfi1[R412X] embryonic mice
Kidneys (both) and testes (both) from male WT and Gfi1mut/mut E17.5 embryos were collected in RNAlater RNA Stabilization Reagent and stored frozen until processing.  RNA was isolated with either the NucleoSpin RNA, Mini kit for RNA purification (kidneys) or RNeasy Plus Micro Kit (testes, 74034, Qiagen) according to the manufacturerâ€™s protocol. Sample quality was assessed using an Agilent Advanced Analytical Fragment Analyzer and Stranded mRNA Library Preparation (1000000124518, Illumina) was performed.