Epigenetic Modifications in the Murine Liver upon Depletion of Transcriptional Coregulator Host Cell Factor 3
ChIP-seq was performed with the 'MicroPlex Library Preparation' kit, from Diagenode 10 ng of immunoprecipitated material was used for sequencing library preparation. Total input was also prepared for sequencing at each time point and replicate. Paired-end sequencing libraries were prepared with the 'MicroPlex Library Preparation' kit, from Diagenode, following the instructions of the manufacturer (Diagenode, catalog no C05010011). No size selection was performed and the chromatin was amplified with 14 cycles of PCR. 100 nucleotides at the fragment ends were sequenced with paired-end sequencing technology from HiSeq 2500 (Illumina).