Deciphering Transcriptome Profiles of Sporadic Medullary Thyroid Carcinoma: An Italian Cohort Study
Deciphering Transcriptome Profiles of Sporadic Medullary Thyroid Carcinoma: An Italian Cohort Study
Patients' tissues collection was performed at Padua Hospital during surgery. The samples were frozen at -80 C° before RNA extraction. Total RNA was extracted from fresh-frozen tissue samples using the Norgen Kit (Biotek Corp, Ontario, Canada), following the manufacturer's instructions. Libraries preparation was performed by using the TruSeq Stranded Total RNA kit (Cat. 20020598, Illumina) according to manufacturer's guidelines and starting from 200 ng of input material.