GSM9475327: Oregon R, Ctrl, intestinal stem cells, rep1; Drosophila melanogaster; RNA-Seq
Intestines were dissected and processed into cell lysates.  After dissociation, cells were filtered through a 40-μm filter and FACS-sorted based on YFP-signal. RNA was extracted using the ArcturusTM PicoPureTM RNA isolation Kit (Thermo Fisher Scientific, 3032689). Libraries were constracted with Illumina for coding transcriptome and sequenced with the AVITI High Output (two-end reads, length 75 bp).