GSM8733647: FACS-sorted bone marrow Eosinophil_RNA_SA4; Mus musculus; RNA-Seq
RNA was precipitated with ice cold absolute ethanol, LPA (Linear Polyacrylamide (Sigma Aldrich) and 3 M Sodium Acetate (NaOAc PH 5.2; ThermoFisher) overnight and resuspended in 10 µl of 10 mM Tris buffer (pH 8.0; Invitrogen). RNA concentrations were measured by flurometric analysis (Qubit). The Quantseq libraries were prepared with an input of 10 ng RNA using the QuantSeq 3' mRNA-Seq Library Prep Kit (FWD) for Illumina (Lexogen) in combination with the PCR add-on Kit for Illumina (Lexogen) (16 amplification cycles) following the manufacturer's protocol. Sample quality was assessed with a Bioanalyzer 2100 (Agilent Technologies)