Illumina WGS of Bordetella bronchialis isolated from CF patient sputum after commencement of phage Simon treatment; day 2 isolate 6
This is one of 58 serial isolates obtained from the same patient described in this Bioproject, from whom the wt B. bronchialis was previously isolated. This isolate was obtained after commencement of phage Simon treatment; day 2 isolate 6. Genomic DNA was extracted from B. bronchialis cultures using the GenFind V3 Reagent Kit (Beckman Coulter) as per manufacturers instructions. Illumina libraries were prepared using the Illumina DNA Library Prep Kit, (M) Tagmentaton (Illumina) and sequenced on the Illumina NovaSeq 6000 platform to produce paired-end reads.