single-cell RNA sequencing of Canis lupus familiaris: adult male MSC
This dataset contains raw single-cell RNA sequencing (scRNA-seq) data of mesenchymal stem cells (MSCs) isolated from three distinct tissue sources of healthy canines: adipose tissue, bone marrow, and umbilical cord. Adipose-derived MSCs were isolated via collagenase digestion, bone marrow-derived MSCs through the whole bone marrow adherence method, and umbilical cord-derived MSCs via the tissue explant method. All cells were expanded in vitro, with P3 (adipose), P4 (umbilical cord), and P5 (bone marrow) passages selected for analysis to ensure a stable proliferative state and a non-senescent phenotype. The cells were validated to exhibit characteristic MSC morphology, trilineage differentiation potential (adipogenic, osteogenic, and chondrogenic), and adherence to the International Society for Cell & Gene Therapy (ISCT) immunophenotypic standards. Following rigorous quality control ensuring cell viability exceeded 95%, single-cell libraries were constructed using the MobiNova-100 platform, encompassing cell capture, lysis, reverse transcription, cDNA amplification, and library preparation. The final libraries were sequenced on the Illumina NovaSeq 6001 platform using a paired-end strategy, generating the raw sequencing reads (FASTQ files) provided in this dataset.