GSM9326521: CD34_AAV; Homo sapiens; RNA-Seq
Day 10 cultured CD34 cells were washed with PBS and viability was determined to be more than 95% by MTT assay. The cell differentiation was determined to Giemsa stain and normal maturation with a CD71/CD235a ratio of 95%. Library was constrcuted by Active Motif using 10x Genomics assay. Briefly, CD34 single-cell GEMs (Gel Beads in Emulsion) were generated on the Chromium Controller, followed by GEM-RT, Dyna Beads cleanup, cDNA amplification and SPRIselect beads cleanup. The Library Bead Kit and Chromium i7 Multiplex Kit was used for generating indexed single-cell libraries for Illumina sequencing. Quality, purity, size and concentrations of cDNA and libraries were determined by Tapestation 2000 (Agilent Technologies, Inc). Libraries were sequenced using the Next-Seq 500 sequencing platform.