GSM9837183: M2 PEMA 2; Mus musculus; RNA-Seq
RNA was extracted using the Direct-Zol™ RNA MiniPrep Plus with Zymo-Spin™ IIICG Columns (Zymo Research Corporation R2070) according to manufacturer instructions. RNA quality and concentration were quantified by the University of Michigan Advanced Genomics Core (AGC) via RNA gel (Agilent TapeStation). AGC conducted library prep with poly-A enrichment and next-generation sequencing (Illumina NovaSeq) according to manufacturer instructions. A TruSeq-based kit was used during library preparation. The adapter sequences for this kit are  R1: AGATCGGAAGAGCACACGTCTGAACTCCAGTCA  R2: AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT  The product name of the library preparation kit is NEB.  This pool was subjected to 151bp paired-end sequencing according to the manufacturer's protocol (Illumina NovaSeq 6000, Control Software Version: 1.8.1, RTA Version: v3.4.4).