RNA seq of MCF-7 slow-cycling persisters on hormone deprivation rep1
RNA was harvested from biological triplicates using RNeasy Universal Plus Mini Kit (Qiagen). RNA was quantified by Qubit, and integrity was measured on a fragment analyzer (Agilent). RNA (200 ng) was hybridized to FastSelect probes (Qiagen) for ri-bo-depletion, followed by library preparation using RNA HyperPrep kit (Roche). Li-braries were pooled for sequencing on a NextSeq550 (Illumina).