GSM9035627: C57BL6_1_Metformin_1; Mus musculus; RNA-Seq
For bulk RNA-seq: mice were perfused with PBS, whole livers minced. RNA was isolated using the Qiagen RNeasy Mini Kit (Qiagen, Hilden, North Rhine-Westphalia, Germany) using the manufacturer protocol. Library preparation was performed using the KAPA mRNA HyperPrep Kit (Roche, Cat# KK8580), which includes poly(A) selection, cDNA synthesis, and ligation of sequencing adapters.