scRNA-seq of HAQ or WT homo sapiens: PBMC treated with adjuvants
Samples were processed using the 10x Genomics Chromium Next Gem Single Cell and Fixed RNA kits. This preparation fixes samples, hybridizes to 18000 human gene probes and produces single cell droplet GEMS with individual barcodes. Libraries were then constructed for Illumina sequencing using the 10x Genomics Dual Index kit, bead size selected and checked for quality before sequencing.