GSM8660251: Transcriptome of Paraescarpia echinospica: plume [ONT]; Paraescarpia echinospica; RNA-Seq
Total RNA was extracted from the plume using the RNeasy Mini Kit (Qiagen) and following the manufacturer's protocol. The RNA concentration was measured using the NanoDrop (Thermo Fisher Scientific) and the RNA integrity was assessed using the RNA Nano 6000 Assay Kit and Agilent 2,100 Bioanalyzer (Agilent Technologies). The following experimental process was carried out according to the standard protocol provided by Oxford Nanopore Technologies (ONT), including sample quality detection, library construction and library quality detection. The final library was sequenced on the Nanopore PromethION platform.