GSM8735682: BRI-1895_TCR_Library; Mus musculus; OTHER
Live CD4+CD19-CXCR5+. Cells were stained with TotalSeq-C Hashtags (Biolegened) and pooled in a single sample before loading. scRNA-seq, bulkRNAseq; Cells were resuspended in 0.4% BSA in PBS at a concentration of 2,000 cells per μl, then loaded onto a single lane (Chromium chip K, 10X Genomics) followed by encapsulation in a lipid droplet (Single Cell 5′kit V2, 10X Genomics) followed by cDNA and library generation according to the manufacturer's protocol. 5' mRNA library was sequenced to an average of 50,000 reads per cell, V(D)J library and HTO (Cell Hashing antibodies) library sequenced to an average of 5,000 reads per cell, all using Illumina Novaseq.