RNA-Seq of Isochrysis galbana HK01: LTLP biological replicate 2
Total RNA was extracted from Isochrysis galbana HK01 cultures exposed to different temperature and phosphorus treatments using the RNAprep Pure Plant Kit (TIANGEN, China). RNA quality was evaluated using an Agilent 5300 Bioanalyzer, and samples with RNA integrity values greater than 6.5 were selected for library preparation. Transcriptome libraries were constructed from high-quality RNA samples and sequenced using the Illumina paired-end sequencing platform. Four biological replicates were included for each treatment group (LTLP, LTHP, HTLP, and HTHP).