RNA-seq of GP-A6-24hpi-1
The wheat transgenic line TaB1#1 and Fielder were cultured until the first leaf was fully expanded. The Bgt isolate E09 was inoculated onto the surface of leaves for 24 hours. Similarly, the barley transgenic line HvB1#22 and GP were inoculated with Bgh isolate A6 and samples were collected at 24 hpi. For each interaction of wheat-Bgt or barley-Bgh, three replicates were performed. The RNA was extracted using the Trizol reagent as described above.