GSM9712785: oScarletHIGH cells from old SP-oScarlet brains (August 2025 experiment); Nothobranchius furzeri; RNA-Seq
After cell collection (from FACS), tubes containing the cells were centrifuged at 300g for 5 minutes at 4˚C. Supernatants were removed to 43.2 μL, and Master Mix was prepared and added to each sample exactly following the instructions in the 10x Genomics Chromium Next GEM Single Cell 3' Reagent Kits v3.1 User Guide. Cells were not counted, in order to load all cells collected in each tube. Once Master Mix was added, the contents of each tube were pipetted up and down (to resuspend the cells fully) and loaded in their entirety onto the 10x chip. Instructions in the 10x Genomics Chromium Next GEM Single Cell 3' Reagent Kits v3.1 User Guide were followed exactly with 11-12 cycles used in the cDNA amplification PCR and 13-14 cycles used in the Sample Index PCR.