RNA-Seq of [Candida] incommunis in YPD
Total RNA was extracted using the RiboPureTM RNA Purification Kit (Invitrogen, Thermo Fisher), according to the manufacturers instructions using approximately 3x108 cells from an exponentially growing culture. Purified RNA was then treated with DNase I to remove contaminating chromosomal DNA. cDNA libraries were prepared using the Illumina Smart-Seq2 kit.