GSM8729592: hepatocytes, obese cord plasma, 24hrs, 15; Homo sapiens; RNA-Seq
Total mRNA was isolated from cord plasma-exposed cells using the RNeasy Mini Kit with on-column DNase treatment per manufacturer instructions and quantified by Qubit and quantitative real-time PCR (qRT-PCR). RNA integrity number (RIN) was determined using the Agilent Technologies 2100 Bioanalyzer and all samples with RIN > 9 underwent RNA-sequencing (RNA-seq). Sequencing libraries of RNA from cord plasma-exposed HepaRG cells were prepared by Novogene Corporation Inc. (Sacramento, CA) using the low input pipeline and sequenced on an NovaSeq 6000 (PE150) to generate 6 Gb of raw data per sample.