TDNA-Seq of Trichoderma atroviride
After the T-DNA and barcode transformation, we performed the Illumina KAPA library preparation protocol to amplify fragments of approximately 300 bp in length. Each fragment contains not only the 20-bp barcode sequence but also an adjacent region of the T. atroviride genome, allowing accurate mapping of each insertion site. These amplified fragments correspond to genomic regions flanking the T-DNA insertion, thus enabling the identification of insertion loci across the genome (constituting the TDNA-seq dataset).