GSM8769107: ShE2F8-2; Homo sapiens; ATAC-seq
ATAC-seq libraries were prepared using the Active motif ATAC-Seq Kit (#53150) according to the accompanying protocol. Library DNA quality was assessed using the Agilent Tapestation 4150 and the High Sensitivity D1000 assay kit while library DNA quantity was assessed using the Invitrogen Qubit 4 Fluorometer and the Invitrogen Qubit dsDNA HS Assay. The quantified libraries were pooled equimolarly, and a total of 25ng were used for Adapter Conversion PCR Amplification using the Universal Library Conversion Kit (App-A), Version: V1.0 (MGI Tech Co., Ltd.), following the Universal Library Conversion Kit (App-A) User Manual (Version: A3). The quality of the purified adapter conversion PCR (AC-PCR) product was evaluated using the Agilent Tapestation 4150 and the High Sensitivity D1000 assay kit, and the quantity was assessed using the Invitrogen Qubit 4 Fluorometer and the Invitrogen Qubit dsDNA HS Assay. DNA denaturation, Single-Strand Circularization, Enzymatic Digestion, Enzymatic Digestion Product Cleanup and Quality Control steps were performed on 1 pmol of AC-PCR product according to the Universal Library Conversion Kit (App-A) User Manual (Version: A3).