TCRdelta repertoire of human Vdelta1 T cells: HIV infection and controls
Deep, quantitative and unbiased amplification of TCR sequences was performed using dimer-avoided multiplex (dam)-PCR, followed by MiSeq sequencing (illumina) performed by iRepertoire, Inc. (Huntsville, USA). The sequencing data were error-corrected and V, D, and J gene usage and complementarity-determining region 3 (CDR3) sequences were identified and assigned.