K18-ACE2-mice-control-rep02 (CRX955795; CRA014776)
Total RNA was extracted from K18-hACE2 and C57BL/6 mouse brain tissues using the TRIzol method. RNA concentration and purity were quantified and detected using Nanodrop; agarose gel electrophoresis was used to detect whether RNA was degraded and contaminated; and an Agilent 5400 instrument was used to accurately detect the integrity of RNA, with a RIN > 8, which meets the criteria for library construction. The total RNA was sent to Novogene in Beijing for transcriptome library construction and deep sequencing on the Illumina Nova PE150 sequencing platform.