LGM484_Surface_27_Jun24 full-length 16S rRNA amplicon from surface seawater collected 2024-06-27
Environmental DNA-based full-length 16S rRNA amplicon sequencing from 500 mL seawater collected at a fixed estuarine-influenced subtropical coastal station south of the Itajai-Acu estuary, southern Brazil. Water was collected with a 10-L Niskin bottle and sequentially filtered through 0.7 um glass fiber and 0.22 um cellulose acetate filters. DNA was extracted with the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit. The 16S rRNA gene was amplified with primers 27F and 1495R with 5-bp barcodes. Libraries were prepared with SQK-LSK114 and sequenced on Oxford Nanopore MinION using Flongle flow cells (FLO-FLG114). Basecalling used the super accurate model and reads were filtered at minimum Q8. Sample details: surface water collected on 2024-06-27; season Winter; internal code LGM484; original sample_id S_27_Jun24; eDNA concentration 14.8 ng/uL; DNA 260/280 ratio 1.8; sequenced reads 460.