RNA-seq of Escherichia phage T7: in vivo 10min post-infection
Total RNA from Escherichia phage T7 was extracted from Escherichia coli MG1655 10 min post-infection, purified by phenol/chloroform extraction, DNase-treated (Qiagen RNase-Free DNase Set), and cleaned (Norgen RNA Clean-Up and Concentration Micro Kit). Polyadenylation followed the ONT protocol (v4, March 2023) using E. coli Poly(A) Polymerase (NEB M0276L). Libraries were prepared using the Direct RNA Sequencing Kit (SQK-RNA004, ONT) without amplification or selection and sequenced on a MinION Mk1B (ONT) with a Flow Cell RNA (FLO-MIN004RA) using MinKNOW v24.06.5.