RNA-Seq of mus musculus: male coronal forebrain section
Spatial transcriptomics libraries were generated from fresh frozen sections using the Visium HD Spatial Gene Expression workflow (10x Genomics) with the Mouse Whole-Transcriptome Probe Kit v2 and Visium HD Reagent Kit. Probe pairs were hybridized to RNA in tissue sections, ligated, released on the Visium CytAssist, captured on Visium HD slides, extended to add spatial barcodes and UMIs, pre-amplified, indexed (Dual Index Kit TS Set A), and SPRIselect-cleaned. Final library constructs (~250 bp) were sequenced on an Illumina NovaSeq X Plus (10B flow cell, PE150) using paired-end, dual-indexed runs: Read 1  28 cycles; i7 index  10 cycles; i5 index  10 cycles; Read 2  90 cycles