Ampliconseq of ITS1 rRNA markers: early successional plant community soil
Fungal communities in soil were profiled using amplicon sequencing of the  ITS1 rRNA region. Fungal ITS1 regions were amplified according to Kljalg et al. (2013) using Illumina overhangcontaining IDT Amplicon PCR primers: forward (5TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG CTTGGTCATTTAGAGGAAGTAA3) and reverse (5GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG GCTGCGTTCTTCATCGATGC3).