GSM9116808: MG_IFNB_2; Homo sapiens; RNA-Seq
Total RNA was isolated from myeloid cells or ~100 mg of tissue using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. RNA samples were treated with 1 unit of DNase I (Life Technologies) at 37°C for 30 minutes to remove contaminating genomic DNA. RNA integrity and concentration were confirmed prior to downstream applications. Reverse transcription was performed using high-quality RNA as input to generate cDNA. For RT-qPCR, cDNA was generated using random hexamer primers. Triplicate qPCR reactions were performed for each sample.