GSM9805066: Gastroid, SPON2 Treated, 1; Homo sapiens; RNA-Seq
Cell pellets were preserved in RNALater and centrifuged at 1000Xg. RNA was extracted using the Qiagen RNeasy Micro Kit with on-column DNase treatment. RNA concentration was assessed by Qubit fluorometry and RNA integrity was determined using the Agilent 4200 TapeStation System. RNA libraries were prepared using the NEBNext UltraExpress RNA Library Prep Kit (NEB, cat. #E3330) with 250ng input per sample. Poly(A) selection was used to enrich for mRNA. Libraries were sequenced on an Illumina NovaSeq X Plus at paired-end 150bp. Done at VANTAGE Core, Vanderbilt University.