RNA-Seq of Perna viridis: adult mantle
Total RNA was extracted from mantle tissue of Perna viridis using standard trizol based methods. RNA integrity and concentration were assessed prior to library preparation. Sequencing libraries were constructed from high-quality RNA following the manufacturers protocol, including mRNA enrichment, fragmentation, first- and second-strand cDNA synthesis, end repair, adapter ligation, and PCR amplification. The prepared libraries were quality-checked and sequenced using a high-throughput sequencing platform.