PacBio RS II sinlge-end Sequencing of KAS22024415 (KAE22249830)
Reference genome of Hibiscus syriacus Baekdansim
Separates Reads Of Insert reads into full-length and non-full-length based on primer and optionally polyA tail detection. The output sequences are stripped of primers and polyA tails and re-oriented. Each output sequence represents exactly one read from a single ZMW single molecule and accuracy for each read varies depending on raw read length and transcript length, which may be anywhere between 85-100%. Time and memory-intensive isoform-level clustering algorithm that outputs high-quality consensus transcript sequences. Every output sequence is an 'isoform consensus' that is the consensus of many different reads that are considered by the algorithm to be the same isoform. Arrow is used to generate the consensus and the associated consensus QVs are used to estimate how good the final consensus accuracy is. Based on the Arrow estimated accuracy and user parameter Default: 99% accurate, the consensus sequences are categorized as HQ high-quality, above 99% or user-defined cutoff and LQ low-quality. http://pacb.com