Con1
There are two main ways to obtain mRNA: one is to use the structural characteristics of most mRNAs in eukaryotes with polyA tails, and enrich mRNA with polyA tails through Oligo(dT) magnetic beads. The second is to remove ribosomal RNA from total RNA to obtain mRNA. Subsequently, the mRNA obtained was randomly interrupted with divalent cations in the NEB Fragmentation Buffer, and the library was created according to the normal NEB library preparation method or the strand-specific library preparation method.