RNA-seq of Porphyromonas gingivalis ATCC 33277 In vitro exponential growth replicate 4
Incubate the filter-sterilized Tryptic Soy Broth with Yeast Extract (TSBYE) supplemented 5 ug/ml hemin and 1 ug/ml menadione with at 37C in anaerobic chamber with an 85% N2, 10% CO2, 5% H2 atmosphere. Regularly monitor bacterial growth and harvest during the exponential phase