GSM9085463: AKPS_iAPC_Divarasib_GNE_7883_rep3; Mus musculus; RNA-Seq
RNA was isolated using RNAeasy kit (Qiagen) and on column DNA digestion. Total RNA was quantified with Quant-iT RiboGreen RNA Kit (Thermo Fisher Scientific) on Victor X2 Multilabel Microplate Reader (PerkinElmer) and quality was assessed using High Sensitivity RNA ScreenTape on TapeStation 4200 (Agilent Technologies). For sequencing library generation, the Illumina Stranded mRNA kit (Illumina) was used with an input of 100 nanograms of total RNA. Libraries were quantified with Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) on Victor X2 Multilabel Microplate Reader (PerkinElmer) and the average library size was determined using D1000 ScreenTape on TapeStation 4200 (Agilent Technologies). Libraries were pooled and sequenced on NovaSeq X Plus (Illumina) to generate 30 millions single-end 50-base pair reads for each sample.