GSM9837600: E15 rat small intestine-derived enteric neural cells, DIV21; Rattus norvegicus; RNA-Seq
Cells were dissociated using Accutase for 3 min and collected into Neurobasal medium. Following centrifugation at 1300 rpm for 3 min, the cell pellet was processed for single-cell RNA sequencing using the 10x Genomics platform according to the manufacturer's protocol. Single-cell RNA-seq libraries were constructed from single-cell suspensions using the Chromium GEM-X Single Cell 5' Kit v3 (10x Genomics), following the manufacturer's protocol.