GSM8640190: PoolC_REP2_DMSO_END; Homo sapiens; OTHER
gDNA from cell pellets was isolated using QIAGEN Gentra Puregene kit. Genome-integrated sgRNA sequences were amplified by PCR using KOD Hot Start Polymerases. i5 and i7 multiplexing barcodes (Ilumina) were added in a second round of PCR and final gel-purified products were sequenced.