Whole-genome CRISPR knockout screen in A549 cell line to identify  host determinants for IAV infection
For PCR amplification products that do not contain any sequencing connector sequence, after passing the quality inspection, 100~500ng DNA fragments were extracted and subjected to End Repair, 5 'Phosphorylation and dA-Tailing, Adapter Ligation, Clean Up, PCR Enrichement and Clean Illumina platform high-throughput sequencing library was constructed by Up and other steps.