RNA-seq of equine uterine extracellular vesicles Day 8 of pregnancy
RNA-Seq library preparation was performed starting from 27 ng total RNA by using the SEQuoia Complete Stranded RNA Library Prep Kit (Bio-Rad Laboratories AG, Cressier, Switzerland) following manufacturers instructions. To deplete ribosomal RNA fragments, QIAseq FastSelect depletion kit (QIAGEN AG) was used in the first step of the library preparation. Sequencing of the 12 libraries was conducted on an Illumina NovaSeq 6000 instrument at the Functional Genomics Center Zurich (FGCZ). Pooled barcoded libraries were run on one lane of a SP flow cell. Paired-end sequencing was performed with 92 bp for read one (cDNA insert) and 8 bp for read 2 (unique molecular identifier (UMI) sequence for removal of PCR duplicates).