GSM8640324: Human iPS cells, Rotenone, 0.3 μM; Homo sapiens; RNA-Seq
Total RNA was extracted using RNeasy Mini Kit (Qiagen). The quantity and quality of the RNA were measured using a Nano-Drop Lite (Thermo Fisher Scientific). Total RNA was used for the construction of sequencing libraries Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on MGI DNBSEQ-T7 sequencing platforms, according to effective library concentration and data amount.