RNAseq of WAT from male offspring born to mothers subjected to various nutritional stress during gestation and/or lactation
RNAseq of WAT from male offspring born to mothers subjected to various nutritional stress during gestation and/or lactation
Mice were euthanized by cervical dislocation under isoflurane anesthesia. Perigonadal WAT was collected prior to immediate freezing in liquid nitrogen and stored at −80 ◦C until analyses. Total RNA was prepared using the RNeasy minikit (Qiagen) Prepared RNAs sample were sent to the sequencing facility (IGENSEQ) at the ICM Institute (Paris, France), and their quality and concentration were checked using bioanalyzer. Libraries were prepared using the \"TruSeq Stranded mRNA\" kit (ILLUMINA®), allowing the construction of a library of polyadenylated RNAs (mRNA and polyadenylated non-coding RNAs such as lncRNA) from total RNAs. The libraries were prepared following the manufacturer's recommendations.