GSM8733937: SK-OV-3_mCherry-EGFP; Homo sapiens; OTHER
The sorted cells (mCherry+ EGFP-) and the parental cells (unsorted cells) were subjected to genomic DNA extraction, PCR-based amplification of sgRNA-encoding sequences and the high-throughput DNA sequencing Agarose gel electrophoresis is used to analyze the degree of DNA degradation and check for RNA or protein contamination. The DNA concentration is then precisely quantified using Qubit. DNA samples with a concentration above 0.5 µg are used for library construction. After end repair and adding an A-tail to the DNA template, adapters are ligated to both ends of the fragments without PCR amplification in the middle, preparing a PCR-free library