GSM9219008: PDAC mouse Kras G12D sample 57614; Mus musculus; RNA-Seq
The dissected tumor tissues were resuspended in digestion buffer (RPMI with 2 mg/ml collagenase type I and 40 U/ml DNase I) and incubated at 37 C for 30 min. Cells were washed and re-suspended in PBS. single cell suspension was loaded on a Chromium controller to obtain single cell GEMS (Gel Beads-In-Emulsions) for the reaction. The library was prepared with Chromium Next GEM single cell 3' kit V2 (10X Genomics) and sequenced on Illumina Novaseq 6000