noninoculated soil sample from chemical fertilizer treatment
Targeted amplification of the V3-V4 hypervariable region of the bacterial 16S rRNA gene from taro rhizosphere and bulk soil, as well as organic fertilizer samples. PCR was performed using primers 341F (CCTACGGGNGGCWGCAG) and 805R (GACTACHVGGGTATCTAATCC). Libraries were constructed using the Illumina 16S Metagenomic Sequencing Library Preparation protocol and sequenced on the Illumina MiSeq platform with 2250 bp paired-end chemistry.