16S rRNA V3-V4 amplicon sequencing of human gut metagenome from 20_DS_0092
V3-V4 region of 16S rRNA gene amplified with primers 341F (CCTACGGGNGGCWGCAG) and 805R (GACTACHVGGGTATCTAATCC) using KAPA HiFi HotStart ReadyMix, 25 cycles. Amplicons purified with AMPure XP beads, indexed with Nextera adapters.