16S rRNA sequencing of Oryzias melastigma: microbiota from whole-body samples of early life stages exposed to diclofenac and meloxicam
Genomic DNA was extracted from whole-body samples of 21 dpf Oryzias melastigma larvae following standard protocols. DNA concentration and purity were measured using spectrophotometry and agarose gel electrophoresis. The V3-V4 regions of the 16S rRNA gene were amplified using specific primers through PCR. PCR products were purified, quantified, and pooled in equimolar concentrations for paired-end sequencing.