GSM8521164: MEFs_MOFKO_RNAseq_Rep2; Mus musculus; RNA-Seq
Total RNA was purified from cells (in three technical replicates) using Maxwell® RSC simplyRNA Tissue Kit (Promega AS1340) according to the manufacturer's instructions. RNA from MEFs were sent to the genomics unit of CRG (Centre for Genomic Regulation, Barcelona) for library construction and sequencing (50bp single end reads) using Illumina HiSeq2500 sequencer. RNA from Hela were sent to BGI (Hongkong) for library (DNBSEQ stranded mRNA library) and sequencing (150bp paired end)