Epigenetic landscape of hormone-independent sexual dimorphism and characterization of canine XIST
Epigenetic landscape of hormone-independent sexual dimorphism and characterization of canine XIST
Eight beagle dogs (four males and four females) were investigated for sexual dimorphism in their transcriptomes. All dogs were five-year-old and had been neutered (castrated or spayed) before one year of age, and their healthy physical conditions were maintained through continuous monitoring, vaccination, and anthelmintic treatment. The experiment was ethically approved by the Institutional Animal Care and Use Committee of National Institute of Animal Science (NIAS 2022-586). Whole blood samples were collected using a 21-gauge needle and 5-mL syringe from the jugular vein of each dog. RNA from whole blood was extracted using a PAXgene Blood RNA Kit (762174; QIAGEN, Germantown, MD, USA) following the manufacturerâ€™s instructions.  Libraries were prepared for 150 bp paired-end sequencing using a TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA).