Cel-Seq of greenlip abalone: VGI1 male ganglia
Individual sequencing of abalone gonad and ganglia transcriptomes was performed by using the CEL-Seq2 protocol, a highly sensitive method for cost-effective sequencing of a large number of single-cell or bulk RNA samples. The initial RNA/primer/ERCC/dNTP mix was prepared using 25 ng of RNA and 0.5 l of the ERCC spike-in mix (1:10,000 dilution). Paired-end sequencing was conducted at the Ramaciotti Centre for Genomics, University of New South Wales, Australia. Custom sequencing was performed on a single lane of a NovaSeq 6000, with a 26 bp read 1, a 95 bp read 2, and a 6bp index read. The reads were demultiplexed using the barcode on read1 and the demultiplexed, single-end read2 are provided here.