GSM9068511: MDSC 17; Mus musculus; RNA-Seq
Sorted MDSCs were collected directly into 500 µL TRIzol reagent (Invitrogen 15596026) and stored at −80 °C. RNA extraction was performed according to the manufacturer's standard TRIzol protocol. RNA integrity was assessed using an Agilent Bioanalyzer, and samples with RNA Integrity Numbers between 8.2 and 10 were used for library preparation. Poly-A-enriched libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina. Libraries were constructed at Fulgent Genetics. Sequencing was performed on an Illumina HiSeq 4000 using 75-cycle single-end reads, generating approximately 20 million reads per sample.