GSM8950872: Q7V4 control rep8; Homo sapiens; RNA-Seq
The isolation of single cells, purification, library preparation, and sequencing were performed at NCI Sequencing Facility. The single cell RNA-seq libraries were prepared using a Chromium Next GEM Single Cell 5' reagent kit v2 dual index protocol according to the manufacturer's instructions. The libraries were pooled and sequenced on a NovaSeq X Plus flowcell (Illumina) using 10 cycles for dual indices, 26 cycles for Read1, and 90 cycles for Read2 non-symmetric run according to the manufacturer's instructions.