GSM8609234: PDGFRb+ MSCs, sham_1; Mus musculus; RNA-Seq
Total RNA was extracted using TRIzol reagent (#15596018CN, Thermo Fisher) from cells isolated from the femoral artiries using FACS, and was eluted in 10 µl of sterile water. SMARTer Ultra Low RNA Kit (Takara, Janpan, Catalog #: 634936) was used to synthesize cDNA. After library amplification, cDNA was tagmented and bar coded using TruePrep DNA Library Prep Kit V2 (vazyme TD503-02). The quantity and quality of final libraries were evaluated using the Qubit 2.0 Fluorometer and Agilent 2100 bioanalyzer.