GSM8608409: THP-1 cells, WT Legionella infection, 5 h post-infection, biol rep 1; Homo sapiens; RNA-Seq
At 5 h post-infection, RNA was extracted from cell monolayers using the ISOLATE II RNA Mini Kit (Bioline Cat# BIO-52073) according to the manufacturer's instructions and analysed using a 2100 Bioanalyzer (Agilent). cDNA libraries were prepared for three independent replicates per condition using the TruSeq® Stranded mRNA Library Prep (Illumina Cat# 20020594) according to the manufacturer's instructions