GSM8948214: 6C; Mus musculus; RNA-Seq
RNA was harvested using RNAeasy minikit columns (Qiagen). mRNA was isolated from 1.2 μg of total RNA using the kit NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). NGS libraries for RNA-seq were prepared from the purified mRNA using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs).