CUT&RUN of mouse Tregs with anti-IgG
After removing adapters using Cutadapt (v2.5), 150 bp paired-end reads were mapped to the reference genome build (mouse, mm10) by using bowtie2 (v2.11.1). For CUT&RUNTools(61), installation instructions are provided by Zhu, Q., Liu, N., Orkin, S.H. et al. at https://bitbucket.org/qzhudfci/cutruntools/src/default/. CUT&RUNTools pipeline was employed for data processing and footprint analysis.