phnJ genes in 30 m (CRX1066003; CRA016611)
DNA was extracted from the filter using the FastDNA Spin Kit for soil (Mo Bio Laboratories, USA) according to the manufacturer's instructions. Quantification of the phnJ and phnD gene copies was performed by quantitative polymerase chain reaction (qPCR) on the total extracted DNA. The primer pair used for the phnJ gene consisted of the forward primer pair phnJ-F CSTATCTSGACGARCAGACSAA and the reverse primer phnJ -R TCGTCGGMGCCCTGRTCGA.