DNA_Seq_Oedothorax_retusus_vacuum_sampling
We followed a two-step PCR metabarcoding approach to amplify a 71 bp                 COI fragment. PCRs included negative controls and extraction controls. Amplicons                 were pooled in approximately equimolar ratios (28 l per sample) according to band                 intensity estimated on a 2 % agarose gel. Final libraries were purified using 1 x                 AmPURE XP Beads. Libraries were pairedend sequenced on a MiSeq Illumina                 system.