RUSB22 'Newton' leaf - MP Nextera MP Gel Plus
The mate-pair libraries were constructed with the Nextera Mate Pair library Sample Prep kit (Illumina CA) followed by the TruSeq DNA Sample Prep kit. The 10X and MP libraries were quantitated by qPCR and sequenced on two lanes for 151 cycles from each end of the fragments on a NovaSeq 6000 using a NovaSeq S4 reagent kit. Fastq files were generated and demultiplexed with the bcl2fastq v2.20 Conversion Software (Illumina) .