GSM8973379: Cortex_FP82_7-days; Mus musculus; miRNA-Seq
Tissues were homogenized in TRIzol Reagent (Invitrogen) and total RNA, including small RNAs, was extracted using the miRNeasy Mini Kit (QIAGEN) following the manufacturer's protocol. RNA quality was assessed using NanoDrop, Qubit, and Agilent Tapestation 2200. Small RNA libraries were prepared using the Illumina TruSeq Small RNA Sample Prep Kit. 3′ and 5′ adapters were ligated to RNA, followed by reverse transcription, PCR amplification, and gel-based size selection. Libraries were pooled and sequenced on an Illumina NextSeq 500 to generate 50 bp single-end reads.