GSM9067965: BT-474_Trastuzumab_Resistance_3; Homo sapiens; RNA-Seq
RNA was isolated with Nucleozol™ (Macherey-Nagel) following the extraction protocol suggested by the manufacturer. RNA was isolated as described above and resuspended in ultrapure water. Concentration and RNA integrity number > 7 were determined and 1 µg per sample was utilized for RNA-seq. RNA libraries were prepared for sequencing using standard Illumina protocols