GSM9116170: 4WUM/KTCN; Homo sapiens; OTHER
The full-thickness corneas were submersed in Optimal Cutting Temperature (O.C.T.) compound (Tissue Tek, Sakura) and immediately frozen on dry ice with the use of Cryospray (Engelbrecht GmbH). The 10 µm-thick two or three consecutive cryosections of one cornea were placed in the Capture Area of a Visium Slide. The slides were stored at -80°C until further analysis. After tissue fixation in methanol, H&E staining, and microscopic imaging the Visium Spatial Gene Expression Libraries were constructed according to the manufacturer's protocol.