ddRAD-Seq of Nanhaipotamon nanriense: gill tissue from Nanhaipotamon nanriense in Fuqing city, Fujian, China
Total genomic DNA was extracted from gill tissue using the Trelief Animal Genomic DNA Kit, following the manufacturers protocol.Double-digest restriction-site associated DNA (ddRAD) libraries were prepared by digesting genomic DNA with EcoRI (G^AATTC) at the Read 1 end and NlaIII (Hin1II, CATG^) at the Read 2 end. Size-selected fragments (300500 bp) were ligated to sequencing adapters and PCR amplified. Paired-end libraries were sequenced on the Illumina NovaSeq 6000 platform (Genepioneer Biotechnologies) to generate 150 bp reads