Se1-Input-G7 (CRX638343; CRA010211)
ChIP-ed DNA and related input samples were tested with spectrophotometer and Qubit fluorometer to inspect the quality. Qualified samples were end-repaired to create blunt-ended, 5'-phsphorylated fragments, and then single dA was added to 3' end of each fragment. Adaptors for high-throughput sequencing were ligated to the dA-tailed fragments, and the library was then amplified by PCR. The libraries with 100-500 bp insert fragments were then inspected with Qubit fluorometer, Agilent bioanalyzer and StepOnePlus Real-Time PCR. Qualified libraries were sequenced with illumine HiSeq platform submitted_instrument_model: Illumina HiSeq X Ten