Illumina NovaSeq 6000 paired-end Sequencing of KAS22024175 (KAE22250530)
Transcriptomic analysis of the mice tissue (spleen, mesenteric lymph node) at 6 weeks after infection of Mycobacterium avium subsp. paratuberculosis by intraperitoneal or oral route.
Extraction of total RNA from mice tissuespleen or mesenteric lymph node was performed by Qiagen RNEasy kit. Sequencing library was constructed with Illumina TruSeq total RNA Sample Preparation kit