GSM9712928: HUVEC shScramble vehicle treated 48h n2; Homo sapiens; RNA-Seq
RNA was extracted using the RNeasy Mini Kit (Qiagen) Sequencing libraries were prepared from 500ng total RNA. Paired-end 150bp read length, NovaSeq 6000 system, SP flowcell and v1.5 sequencing chemistry. using the TruSeq stranded mRNA library preparation kit (cat# 20020595, Illumina Inc.) including polyA selection. Unique dual indexes (cat# 20022371, Illumina Inc.) were used. The library preparation was performed according to the manufacturers' protocol (#1000000040498)