WGS of a serie of Arabidopsis thaliana tetrad's products to study the role of RECQ4A, RECQ4B, FIGL1 and FANCM genes in the rate, nature and distribution of meiotic recombination products (CO and NCO)
WGS of a serie of Arabidopsis thaliana tetrad's products to study the role of RECQ4A, RECQ4B, FIGL1 and FANCM genes in the rate, nature and distribution of meiotic recombination products (CO and NCO)
Parental Col-0 and Ler-1 and F1 growth: A. thaliana plants were grown in greenhouses with 70% humidity and under a 16 h/8 h day/night photoperiod with temperatures of 19 °C during the day and 16 °C at night.  Wild-type Col-0 and Ler-1 are 186AV1B4 and 213AV1B1 from the Versailles A. thaliana stock centre (http://publiclines.versailles.inra.fr/).The following mutations were used in this study: in Col-0, fancm-1 4, figl1-16, recq4a-4 (N419423) and recq4b-2 (N511130) 28, apt1-3 20, qrt1-2 (CS8846)18; in Ler-1, fancm-10 6, figl1-12 6,  recq4a-W387X 5, and qrt1-1 (CS8845)18. Flowers from the F1 obtained by crossing CS8846 and CS8845 were tapped on a glass slide to deposit tetrads of pollen grains. Using a needle, a single tetrad was taken and gently embedded in the stigma of a apt1-3 Col-0 plant. The fertilized plant was grown in a greenhouse without other Arabidopsis plants to avoid cross-pollinization. Seeds from fruits having exactly four seeds were sterilized a few seconds in a drop of 70% ethanol and sown on solid medium 29  without sugar.  After stratification for 2 days at 4◦C, plants were grown in long day conditions. At the four leaves stage, the plants were transferred on soil and grown in green houses until the induction of the first hamp.   Illumina TruSeq