scRNASeq of adult Mus musculus HSC (Tet2KO_H04)
scRNA-seq data were generated from Tet2-/- mice and WT littermate controls. ESLAM HSCs were FACS-purified. Freshly isolated HSCs were subjected to single-cell RNA SmartSeq2 sequencing (2 x 96-well plates, WT and Tet2-/- mice, one mouse per plate). RNA was extracted using the Picopure RNA isolation kit (Thermo Fisher). Libraries were prepared using a protocol adapted from the msSCRB-seq workflow and quality control was performed using the Bioanalyzer system (Agilent).