GSM9805097: SpheroidsFBS_1; Sus scrofa; RNA-Seq
Cells were collected and lysed/preserved in 1X Zymo DNA/RNA Shield according to the Plasmidsaurus RNA-Seq sample preparation instructions. Poly(A)+ mRNA was captured using oligo-dT priming, followed by reverse transcription, second-strand cDNA synthesis, tagmentation, library indexing with unique dual indices, and PCR amplification to add Illumina P5/P7 adapter sequences.