GSM9115504: Multiplexed sample: Cyst_1 (BC_001), Cyst_2 (BC_002), MRONJ_1 (BC_003), and MRONJ_3 (BC_004); Homo sapiens; RNA-Seq
Single-cell gene expression profiling was performed using the Chromium Fixed RNA Profiling workflow (CG000527; 10x Genomics). Library preparation involved the Chromium Fixed RNA Kit (Human Transcriptome) and the Chromium Next GEM Single Cell Fixed RNA Sample Preparation Kit Following hybridization of detection probes to RNA within fixed cells, adjacent probes were joined through a ligation reaction. Samples were then pooled to normalize cell counts across groups and processed on the Chromium X instrument, where cells were encapsulated with barcoded primer beads.