GSM8490747: MS_1_condyle_12weeks; Mus musculus; RNA-Seq
Both the condyles and synovial tissues were stored at -80°C until RNA was extracted using TRIzol reagent (Invitrogen). After homogenization with zirconia beads in a TRIzol-containing tube, the mixture was incubated and then centrifuged. The supernatant was removed, RNA was isolated, and the total RNA was dissolved in RNase-free water. RNA was harvested using the Direct-zol™ RNA MicroPrep kit (ZYMO RESEARCH). A total of 0.2 μg of RNA was used for the construction of sequencing libraries RNA libraries for RNA-seq were prepared using Optimal Dual-mode mRNA Library Prep Kit following manufacture's protocols.