RNA-Seq of Panax notoginseng
For transcriptome sequencing, roots and shoots were collected from CK, +Cd, and +Cd+Si treatments. Total RNA was extracted using TRIzol reagent according to manufacturer's instructions (Magen). RNA concentration and purity were assessed were assessed using a Nanodrop ND-2000 spectrophotometer (Thermo Scientific, USA), and RNA integrity was evaluated using an Agilent Bioanalyzer 4150 (Agilent Technologies, CA, USA). High-quality RNA samples were used for library construction. Paired-end libraries were prepared using the ABclonal mRNA-seq Library Preparation Kit (ABclonal, China) according to the manufacturers protocol. Sequencing was performed on an Illumina NovaSeq 6000 platform by Shanghai Applied Protein Technology Co., Ltd.