GSM8697658: Fig2a_VDJ_JP0037; Mus musculus; RNA-Seq
Single cell suspension from lymph nodes was prepared in PBS supplemented with 0.5% BSA and 2 mM EDTA (PBE). Live GC cells were sorted using BD FACSymphony S6 or BD S8 FACSDiscover sorter Library was  generated using a Chromium Next GEM Single Cell 5' Reagent Kit v2 or v3(Dual Index) with Feature Barcode technology for Cell Surface Protein, according to the manufacturer's protocol.