Pterocarpus angolensis: Whole-genome shotgun sequencing raw reads
Genomic DNA was extracted from silica-dried and herbarium-preserved plant samples. DNA extraction from herbarium tissue followed an adapted protocol of Cappellini et al. (2010), while silica-dried samples used the DNeasy Plant Mini Kit (QIAGEN). Extracts were fragmented to ~400 bp and used to prepare genomic libraries with the NEBNext Ultra II DNA Kit. A portion of each library was retained without enrichment for shotgun sequencing. Sequencing was performed using 2 x 150 bp paired-end reads on an Illumina NovaSeq 6000 platform, targeting ~2 million reads per sample. Demultiplexed and adapter-trimmed FASTQ files were provided by the sequencing platform.