GSM8768255: Sample 4 - Progressive Sarcoidosis; Homo sapiens; RNA-Seq
Bronchoscopy with BAL was performed as previously described.(9, 10). Briefly, four to eight 60 ml aliquots of sterile normal saline at room temperature were instilled into the airway with a syringe then aspirated using low suction. For each collection from an individual patient, the aspirated BAL specimens were pooled, kept on ice, and processed within one hour of collection. The BAL cells were obtained by centrifugation and cryopreserved. Cryopreserved cells were thawed and captured on 10X Chromium in the Genomics Core at National Jewish Health using 10x Genomics Single Cell 3′ v3 chemistry, followed by sequencing on Illumina NovaSeq.