GSM9394219: ISW2_AMB_1h_R1; Candida albicans; RNA-Seq
Total RNA was extracted using an RNAeasy purification kit (Qiagen) and glass bead lysis in a Biospec Mini 24 bead-beater. For ChEC-seq, DNA was purified using the MasterPure Yeast DNA Purification Kit (Epicentre) following the manufacturer's instructions and resuspended in 50µl of 10 mM Tris-HCl buffer (pH 8.0). For MNase-seq, DNA was extracted using phenol-chloroform-isoamyl alcohol and precipited with sodium acetate and ethanol. The NEBNext UltraTM II RNA Library Prep Kit for Illumina was used to construct the RNA-seq library. For the ChEC-seq and Mnase-seq, sequencing libraries were prepared using the KAPA HyperPrep Kits.