RNA-seq of Human Prostate Cancer cell lines (LNCaP and 22RW1) with T2ERG expression
RNA-seq of Human Prostate Cancer cell lines (LNCaP and 22RW1) with T2ERG expression
Total cellular RNA was extracted using the RNeasy mini kit (Qiagen). 22Rv1 (CVCL_1045) and LNCaP-FGC (CVCL_1379) were grown in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS) (Gibco), 1% L- glutamine (Gibco), and 1% penicillin-streptomycin (Gibco).  For DOX-inducible overexpression, the gene of interest was cloned into pCW57.1 (#41393, Addgene). Cells were induced with full medium containing EtOH or DOX for 4 days (medium changed at 2 days) or 1 day.  Total cellular RNA was extracted using the RNeasy mini kit (Qiagen) including on-column DNase I digestion and quantified using the Qubit RNA Broad-Range Assay Kit (Thermo Fisher Scientific).  Poly-A-containing mRNA isolation and library preparation were performed by using the TruSeq Stranded mRNA Library Prep (Illumina) and the AMPure XP SPRI Reagent (Beckman Coulter).