GSM8869860: Bell_B, rep2; Mus musculus; RNA-Seq
Tooth germs at cap and bell stage were dissected from mouse mandible. The tooth germs from mouse mandible at cap and bell stage were divide two region with lingual and buccal part with 30 gauge fine needle. Furthermore, Lingual part of tooth germs and buccal part of tooth germs were cultured in vitro for 2 days. The mesenchymal cells were isolated from each tooth germ with dispaseII. The total RNA was extracted with TRIzol. RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.