GSM8640206: Ant1(Tg)/+_female_quadricep_muscle_1; Mus musculus; RNA-Seq
Total RNA was extracted using Trizol/chloroform phase separation followed by RNeasy Mini kit column purification. The quality and quantity of RNA was assessed with the Bioanalyzer. 500ng RNA was used as input to the Illumina Stranded mRNA kit for the construction of sequencing libraries. RNA libraries were prepared using the Illumina Stranded mRNA prep kit following the manufacturers protocol.