Genome of Bacillus thuringiensis strain akera
An amplification-free long-read sequencing library was constructed using kit v14 chemistry (Oxford Nanopore Technologies). Subsequently, nanopore sequencing was performed utilizing R10.4.1 flow cells to obtain raw genetic data from the samples. The lowest-quality 5% of FASTQ reads were removed using Filtlong v0.2.1 (2) with default settings. Remaining reads were downsampled to 100 coverage and assembled using Flye v2.9.1 (3), optimized for high-quality input. The resulting assemblies were polished with Medaka v1.8.0 (4) and annotated using Bakta (5). Assembly contigs were visualized with Bandage v0.8.1 (6), and genome completeness and contamination were assessed with CheckM v1.2.2 (7). Species identification was carried out using Mash v2.3 (8), Sourmash v4.6.1 (9), and CheckM v1.2.2 (7)