Whole-genome sequencing Nanorana quadranus
Genomic DNA was extracted from the muscle tissue. Library construction was performed using the VAHTS Universal Plus DNA Library Prep Kit for Illumina (ND617). The required concentration was 1 ng/l, with the fragment size centered around 430-530 bp and an average fragment size between 420-580 bp. After passing the quality check, the library was sequenced on the Illumina NovaSeq 6000 platform with a PE150 sequencing strategy.