GSM9036265: CHD_VDJ; Homo sapiens; OTHER
Peripheral mononuclear blood cells (PBMCs) were freshly isolated from EDTA blood following density medium centrifugation. Isolated cells were gently frozen in a freezing medium containing 90% heat-inactivated fetal calf serum and 10% DMSO, and stored in aliquots at −80 °C until further processing. The PBMCs were thawed, and fluorescence-activated cell sorting (FACS) was performed to isolate live of αβ and γδ T cells. These sorted T cells were then used to generate single-cell RNA sequencing (scRNAseq) libraries with the Chromium Next GEM Single Cell V(D)J Reagent Kits v2 following the manufacturer's protocol (10x Genomics). The scRNAseq libraries were sequenced on the NovaSeq X platform, targeting 25,000 read pairs per cell