sequences of bacteria
Dental caries plaque samples were collected from carious enamel lesions of patients at Tianjin Stomatological Hospital. After resuscitation, samples were cultured in BHI medium supplemented with 1% sucrose, treated under conditions consistent with in vitro antibacterial assays, and then subjected to genomic DNA extraction. The V3-V4 region of the 16S rRNA gene was amplified, and the sequencing library was constructed using standard Illumina library preparation protocols for 16S rRNA amplicon sequencing.