RNA-seq of human retinal organoids: normal control
The enriched mRNAs by Oligo(dT)-coated magnetic beads were fragmented into short segments and then used as templates to synthesize the first cDNA with random hexamer primers, and the second cDNAs were subsequently synthesized with dNTPs and enzymes in buffer.The  double-stranded cDNAs were purified, followed by end repair, A-tailing, and ligation of sequencing adapters.Next after being screened, the fragments were amplified by PCR to get enriched cDNA libraries.