Defined human tri-lineage brain microtissues
Library construction was conducted using the Chromium Next GEM Single Cell 3 Library Kit v.3.1 (10 Genomics, 1000128). All reaction and quality control steps were carried out according to the manufacturers protocol and with recommended reagents, consumables and instruments. We chose 11 PCR cycles for library generation. Quality control of cDNA and libraries was conducted using a bioanalyzer (Agilent) at the Stanford Protein and Nucleic Acid Facility.