singel cell transcriptomic in colon cancer
Single-cell RNA libraries were prepared using the Chromium Fixed RNA Profiling reagent kits (10x Genomics). Approximately 8,000 cells per sample were loaded into the Chromium Controller for encapsulation into gel beads-in-emulsion (GEMs) for single-cell RNA profiling. Each GEM contains unique barcodes for the identification of RNA from individual cells, and unique molecular identifiers (UMIs) for precise quantification of RNA molecules. After cell partitioning, GEMs were subjected to reverse transcription, where fixed RNA molecules were converted into cDNA within each GEM. Following cDNA amplification, the single-cell libraries were prepared according to the Chromium Fixed RNA Profiling workflow, which involves enzymatic fragmentation, end-repair, adaptor ligation, and final PCR amplification of the cDNA libraries