S aureus isolate 5AD1Up6
DNA was extracted using the KingFisher Flex Purification System (Thermo Fisher, Massachusetts) quantified with the Quant-it picogreen dsDNA assay kit (Cat. No. P7589, Invitrogen, Massachusetts), and normalized by concentration. Libraries were prepared using the Illumina DNA prep (M) Tagmentation kit (Cat No. 20018705, California), pooled equimolarly, and sequenced as paired-end 150-bp reads using the Illumina Novaseq 6000 System with the S1 300 cartridge and flow cell (Illumina, California).