GSM9115943: M2B226_2; Mus musculus; RNA-Seq
Total RNA was extracted with Trizol (Invitrogen).The cDNA was synthesized from the extracted RNA using a reverse transcription kit,and labeled with fluorescent dyes.The purified DNA was denatured at high temperature (typically at 95°C for 3 minutes), followed by hybridization of the labeled cDNA to the mouse gene expression microarray chip(Agilent) according to the manufacturer's instructions.The hybridization was then carried out in a hybridization oven at 42°C for 16-20 hours.The microarray chip was washed to remove unbound cDNA using the recommended buffers and then scaned to detect fluorescence signals. RNA-seq libraries were generated and sequenced by CapitalBio Technology (Beijing, China).