GSM9035692: HHL5 cells transfected with Flag-DDX49 following OA/PA treatment for 24 hours, replicate 1, RIP-seq; Homo sapiens; RIP-Seq
The cells were cross-linked using ultraviolet light and harvested. RIP was performed using the Magna RIP® RNA-Binding Protein Immunoprecipitation Kit (Millipore) according to the manufacturer's instruction. Cell lysates were subjected to RIP by the indicated antibodies. The co-immunoprecipitated RNAs were isolated by TRIzol for sequencing. RIP-seq was performed by Biotechnology Corporation (Shanghai, China)