GSM8950770: WT_bleo_MicroC_biol_rep2; Escherichia coli; OTHER
Cells were fixed with 3% formaldehyde and 2 mM DSG followed by treatment with lysozyme. DNA was digested with MNase. DNA ends were labeled with biotin and ligated followed by removal of biotin from non-ligated ends by Exonuclease III. The sample was de-crosslinked at 65 °C in the presence of Proteinase K followed by phenol-chloroform extraction and ethanol precipitation. RNA was removed by RNase A treatment and DNA was further purified with AMpure XP beads. Downstream steps included sonication, biotin pull down, end repair, A-tailing, ligation of Illumina Y adapters, and PCR amplification.