GSM9395665: Blastocyst, Str - let7f, rep9; Mus musculus; RNA-Seq
Embryos were pooled into samples of N = 10 to provide adequate material to isolate high-quality RNA. Total RNA was extracted from frozen embryos using the PicoPure™ RNA Isolation Kit (Applied Biosystems, KIT0204). Libraries of embryo rRNA-depleted total RNA were prepared with the SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Takara Bio, 634486).