GSM9069591: Visium CytAssist Spatial Gene and Protein Expression, GEX, PBC-PR_6835-5A; Homo sapiens; OTHER
Two formalin-fixed & paraffin-embedded (FFPE) malignant mesothelioma tissue blocks were obtained from National Meosthelioma Virtual Bank. Malignant mesothelioma samples were assessed for RNA quality, and all samples had a DV200 score above the minimum threshold ≥30%. Spatial transcriptomic and proteomic libraries were generated with Visium CytAssist for FFPE v2 gene expression kit (10x Genomics: 1000520) with the Human Immune Cell Profiling Panel (10x Genomics: PN-1000607). 5 micron FFPE sections were placed on Schott Nexterion Hydrogel Coated Slides (Schott North America: 1800434) or Fisherbrand Superfrost Plus Slides (Fisher: 22-037-246) and processed through the 10x CytAssist for FFPE v2 protocol according to the manufacturer's instructions. H&E images were taken at 10x magnification using an EVOS 7000 Imaging System (Thermo Fisher Scientific).