heart trans of Du an catfish
Total RNA was extracted separately from  heart tissues and purified for transcriptome sequencing. Poly(A)+ mRNA was enriched using Oligo(dT) magnetic beads, fragmented and reverse-transcribed into first-strand cDNA using random primers. After second-strand cDNA synthesis and PCR amplification, cDNA fragments of approximately 380 bp were selected to construct three RNA-seq libraries, one for each tissue. The libraries were sequenced on the BGI T7 platform using a paired-end 150 bp strategy.