GSM9837474: Donor 3, vehicle; Homo sapiens; RNA-Seq
Migratory cells were incubated in 0.05% Trypsin-EDTA (Gibco, #25300054) for 15 minutes, then passed through a 40 µm cell strainer. A total of 3,400 viable cells/μL were resuspended in Cell Suspension Buffer for library preparation using the Illumina Single Cell 3' RNA Prep, T10 kit (Illumina, #20135691) following the manufacturer's protocol.