GSM8554314: CG_UNTREATED_D25_REP1 + CG_UNTREATED_D25_REP2 + CG_UNTREATED_D25_REP3 GEX; Homo sapiens; RNA-Seq
Multi-omic scRNA/ATAC-seq Single-cell suspensions were processed using the 10x Genomics Single Cell 3' v3.1 workflow following the manufacturer's instructions. Cells were cryopreserved in CryoStor CS10. Vials were thawed in a water bath at 37°C and warm DMEM was added dropwise. Cells were washed once with DMEM and resuspended in PBS+10% FBS. Cells were counted and biological replicates were barcoded with distinct CELLPLEX labels and the same number of cells from each sample was pooled. Samples were then processed for multi-omic scRNA/ATAC-seq analysis using the 10x Genomics Single Cell Multiome ATAC + Gene Expression v1 workflow.