Long read DNA sequencing library for JR9T
Skin and brain samples were collected as soon as possible after euthanasia and placed in on ice and then frozen at -80C until DNA extraction. DNA was extracted from frozen 1-3 mm cubes of tissue, using the DNeasy Blood and Tissue Kit (Qiagen, Valencia, CA). Whole genomeDNA sequencing were prepared for all samples using the Native Barcoding Kit (Oxford Nanopore Technologies (ONT), Oxford, UK) and were sequenced on the ONT PromethION 2 Solo device using R10.4.1 flow cells