GSM9180737: Proximal colon, HSCR mouse, rep 1; Mus musculus; RNA-Seq
RNA was extracted from longitudinal muscle and myenteric plexus tissues isolated from the proximal and distal colon segments of Ednrb -/- and wildtype mice using the RNeasy Kit (Qiagen) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dTTP for non strand specific library or dUTP for strand specific library.For the non strand specific library, it was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.For the strand specific library, it was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.