Ministeria vibrans DSM_114853 monoxenic culture with Thalassospira lucentensis strain isolated from Ministeria vibrans ATCC_50519 xenic culture
The 16S rDNA V4 variable region was amplified using PCR primers 515 (Caporaso; GTGCCAGCMGCCGCGGTAA) and 806 (Caporaso; GGACTACHVGGGTWTCTAAT) as part of the NEXTFLEX16S V4 Amplicon-Seq Kit 2.0 for library preparation and DNA purified using Agencourt AMPure XP Magnetic Beads. Paired-end sequencing was performed at MR DNA (www.mrdnalab.com, Shallowater, TX, USA) on a MiSeq Nano v2 (500 cycles).