GSM9617763: E18.5_RNA_Mutant_Male_1; Mus musculus; RNA-Seq
E145 scRNA: Single cell suspensions were prepared from mouse embryonic kidneys using a cold-active protease cell dissociation protocol (Adam et al. Development 2017). Foxd1+ progenitors and their derivates were subsequently isolated by fluorescence-activated cell sorting (FACS). E185 scRNA: Single cell suspensions were prepared from mouse embryonic kidneys using a cold-active protease cell dissociation protocol (Adam et al. Development 2017). Foxd1+ progenitors and their derivates were subsequently isolated by fluorescence-activated cell sorting (FACS). E145 scATAC: Single cell suspensions were prepared as for scRNA-seq. Nuclei was subsequently isolated using the protocol from 10x Genomics. E185 bulk-RNA: RNA was extracted using the RNeasy Mini Kit (Qiagen 74104). E145 scRNA: Libraries were constructed using the 10x Genomics Chromium 3' Kit version 3. E185 scRNA: Libraries were constructed using the 10x Genomics Chromium 3' Kit version 3. E145 scATAC: Libraries were constructed using the 10x Genomics Chromium Single Cell ATAC Kit version 2. E185 bulk-RNA: libraries were constructed using the Illumina Stranded mRNA-seq Library Prep Kit.