GSM9085530: immune profiling,sfbneg, class-switched pp b cells, GEX, rep1; Mus musculus; RNA-Seq
For PP B cell immune profiling, IgA⁺, IgG1⁺, or IgG2b⁺ class-switched B (CSB) cells (CD45⁺CD19⁺TCRβ⁻Isotype⁺) from SFB-negative and SFB-positive Taconic WT mice (five mice pooled per group) were individually FACS-sorted. The three isotype-positive CSB populations were then mixed to reflect their natural ratio in PPs from SFB-negative and SFB-positive mice. The sorted and pooled cells were subsequently stained with the following TotalSeqTM C antibodies (BioLegend): IgD (Cat# 405747), IgM (Cat# 406541), CD62L (Cat# 104455), and CD38 (Cat# 102735), and processed using the Chromium Next GEM Single Cell 5′ Library & Gel Bead Kit v2 (10x Genomics) along with the 5′ Feature Barcode Library Kit and Mouse B Cell V(D)J Enrichment Kit, according to the manufacturer's instructions.