GSM9036233: Intestine, nod2-/-, nod2-def, nod2ko,nod2-deficient; Danio rerio; RNA-Seq
Intestinal tissue was dissociated enzymatically and mechanically followed by removal of debris and dead cells using OptiPrep™ Density Gradient Medium (Sigma). single cell RNA libraries from freshly collected cells were prepared and barcoded with a 10X genomics Chromium Next GEM Single Cell 3' Reagents Kit v3.1.