miRNA-Seq of mus musculus: serum
Total RNA was extracted using Qiagen serum/Plasma Kit-Qiagen#217184 following the manufacturers instructions.According to the experimental instructions, the purified Total RNA was subjected to 3 'end-connector connection, 5' end-connector connection, reverse transcription, amplification, cDNA library size selection, purification and other steps to complete the construction of sequencing sample library.The library was constructed using Qubit 2.0 Fluorometer for concentration and Agilent4200 for library size.Illumina NovaSeq6000 sequencer was used for sequencing in PE150 mode. Quality control standard: The number of reads after sequencing for each sample was about 20 M; The proportion of base mass greater than 20 (Q20) is not less than 90%.