Bulk RNA seq data from mice lacking ACBP in brown and white adipose tissue
Bulk RNA seq data from mice lacking ACBP in brown and white adipose tissue
All mice were fasted two hours prior to sacrifice. Depending on subsequent experimental requirements mice were sacrificed by either cervical dislocation or anesthesia followed by heart puncture to draw blood. Anesthesia used: 25% hypnorm (0.315 mg fentanylcitrate, 10 mg of fluanisone, 0.5 mg methylparahydroxybenzoate and 1 mL sterile milliQ water) and 25% midazolam (Midazolam, 5 mg/mL) in sterile milliQ water, dosage: 0.01 mL/g mouse. EDTA plasma and tissues of interest were harvested and snap frozen in liquid nitrogen. Tissues were homogenized in TRI Reagent (Sigma) and total RNA was extracted in accordance with the manufacturerâ€™s protocol. Sample preparation was performed as described in the NEBNext Poly(A) mRNA Magnetic Isolation Module kit and NEBNext Ultra II RNA Library Prep Kit E7770 (New England Biolabs, Ipswich, MA, USA) using 500ng input RNA. The amplified libraries were validated by Agilent 2100 Bioanalyzer using a DNA 1000 kit (Agilent Technologies, Inc., Santa Clara, CA, USA) and quantified by qPCR using the KaPa Library Kits (KaPa Biosystems, Wilmington, MA, USA).