snRNAseq-mouse placenta-FB
Mouse placentas were generated by transferring E3.5 blastocysts conceived either in vivo (FB) or by in vitro fertilization (IVF) into pseudopregnant recipients. On embryonic day 12.5, placentas were collected, flash-frozen, and processed for single-nucleus RNA sequencing. Nuclei were isolated with the Singulator 100, counted, and barcoded using the 10X Genomics Chromium Next GEM Single Cell 3 v3.1 kit. Libraries were sequenced on an Illumina NovaSeq 6000. Data processing included quality control, clustering, and differential gene expression analysis to compare IVF and FB placentas.