snATAC_Patz1_Uninjured
Single-cell ATAC-seq datasets were generated using the Chromium Single Cell ATAC kit from 10x Genomics, following the manufacturer's instructions (Catalogue Number: CG000496 chromium next GEM single cell ATAC reagent kit v2). Nuclei were isolated from the motor cortex of mice (n=3 per sample). The isolated nuclei suspensions (3,000-5,000 nuclei) were then added to the transposition mix, which contains the transposase enzyme responsible for DNA fragmentation and the addition of adapter sequences to the fragmented ends. GEMs were generated on the Chromium Next GEM Chip H by adding barcoding reagents and enzymes and partitioning oil to the transposed nuclei. The recovery and purification of the generated GEMs were performed using streptavidin beads, and size selection was carried out with SPRIselect beads and a series of ethanol washes. After cleanup, the DNA fragments were eluted using an elution buffer, followed by indexing PCR and final cleanup before sequencing on the Illumina platform.