GSM8900695: Peripheral blood CD14+ monocyte, Healthy Control, Rep2  - Input; Homo sapiens; ChIP-Seq
Cells were fixed in 1% methanol free formaldehyde, and then lysed. Chromatin samples were sonicated into 200-600 bp long fragments. Chromatin was split into the 10% input control, and the rest was used for either H3K4me3 or H3K27me3 chromatin immunoprecipitation. Input and ChIP'd DNA samples were extracted using the Qiagen MinElute PCR purification kit, as per manufacturer's instructions. DNA libraries were prepared for sequencing using the NEBNext Ultra II Directional DNA Library Prep Kit for Illumina (New England BioLabs) following the manufacturer's instructions. Quality control was performed on the DNA libraries using a high sensitivity DNA Chip (Agilent) on the 2100 Bioanalyzer Instrument (Agilent). ChIP-Seq (Each sample was sequenced to a depth of ~30 million reads, with fragments undergoing single end sequencing of 75 base pair read lengths.)