GSM9180779: Eomes+IL10- (P2) rep 1; Mus musculus; RNA-Seq
Spleens (n = 16) were taken on day +14 and pooled from every 4 mice leading to 4 pooled samples. CD4+ T cells were enriched with MACS selection, stained with CD90.2 Pacific Blue, CD4 APC/Cy7, H2Dd AF647 and FACS sorted to P1 (Eomes- IL10-), P2 (Eomes+ IL10-) and P3 (Eomes+ IL10+) based on the expression of mCherry and GFP. Sort-purified CD4+ T cell subsets (350 – 500 x10^3 cells per sample) were stored in TRIzol reagent and sent to Macrogen (Seoul, South Korea) for bulk RNAseq.