16S rRNA gene sequencing of Pontastacus leptodactylus gut microbiota, DMPT supplementation group, replicate 1
Gut content samples were collected from Pontastacus leptodactylus after 4 weeks of feeding with different dietary treatments (all-plant protein diet, all-animal protein diet, all-plant protein diet supplemented with 0.5% DMPT). Microbial genomic DNA was extracted from intestinal contents, and the V3-V4 hypervariable region of the 16S rRNA gene was amplified by PCR. Paired-end sequencing was performed on the Illumina PE250 platform to construct the sequencing library.