Exp2-6 (CRX1158718; CRA018340)
Genomic DNA was extracted from 200 mg of the fecal samples using Zymoresearch Fecal/Soil DNA isolation kit (Zymoresearch, Irvine, CA) following the manufacturer's instructions. The DNA yield, and quality were determined using NanoDrop One (Thermo Scientific), and 1% agarose gel electrophoresis, respectively. Subsequently, PCR products were measured using the Qubit dsDNA HS assay Kit (Q32854, Invitrogen) and pooled equally for the library construction.