ONT long-read sequencing of soil metagenome: ACT pot trial, Kowen Forest soil, no plant, water, R01
Shotgun metagenomic DNA libraries from soil and rhizosphere (rhizosheath) samples were extracted with the QIAGEN DNeasy PowerSoil Pro kit, prepared with the ONT Native Barcoding Kit 96 V14 (SQK-NBD114.96) using end-prep and ligation, pooled and sequenced on an ONT PromethION, then basecalled and demultiplexed with Dorado v1.1.1 in super-accurate (sup) mode and quality filtered (median Q>=15, length >=300bp).