rice_SB_RNAseq_E4 (CRX719314; CRA011415)
After enrichment of eukaryotic mRNA with a polyA tail by magnetic beads with Oligo(dT), the mRNA was broken up with ultrasound. The fragmented mRNA was used as a template and random oligonucleotides were used as primers to synthesise the first strand of cDNA in the M-MuLV reverse transcriptase system, followed by degradation of the RNA strand with RNaseH and synthesis of the second strand of cDNA with dNTPs under the DNA polymerase I system. The purified double-stranded cDNA was end-repaired, A-tailed and sequenced with AMPure XP beads, and the cDNA of about 200 bp was screened with AMPure XP beads, PCR amplified and the PCR product was purified again with AMPure XP beads to obtain the final library. submitted_instrument_model: Illumina NovaSeq 5000