GSM9395558: PNR_35_naive_cd4+_baseline; Homo sapiens; RNA-Seq
Total RNA from CD4+ T cells at baseline and after four weeks of Nicotinamide Riboside supplementation were extracted with the miRNeasy Micro Kit (Qiagen) and RNA quality and integrity was assessed by Qubit 3.0 fluorometer (Thermo Fisher Scientific) and Agilent Bioanalyzer (Agilent Technologies). Libraries were prepared using TruSeq stranded Total RNA HT kit (Illumina) and sequenced in a HISeq 3000 (Illumina)