GSM9115546: 10x 3' GEX (blood, bone marrrow, and spleen from MaGIC mice); Homo sapiens; RNA-Seq
Sorted cells based on expression of human CD45 and human CD66b from blood and spleen of MaGIC humanized mice were incubated with barcoded hashing antibodies (TotalSeqB0251, TotalSeqB0252; BioLegend) and pooled with cells from bone marrow to a final concentration of 1,500 cells/µL and loaded onto the 10x Chromium Controller using the Chromium Next GEM technology. Library preparation was performed in-house using the Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (10x Genomics), including cell surface feature barcoding. Sequencing was conducted on the NovaSeq 6000 system (Illumina).