GSM8733097: C57BL/6 mouse, cadmium-exposed, Day90, rep3; Mus musculus; RNA-Seq
Total RNA was extracted from liver tissues using TRIzol reagent (Invitrogen, Grand Island, NY, USA). After determining RNA concentration with Qubit (4.0) and assessing RNA quality by agarose gel electrophoresis techniques. An adequate quantity of RNA was interrupted to 200-500 bp using metal ions, then purified and solubilised. Next, an anti-m6A antibody (Danvers, MA, USA) was added for immunoprecipitation, which binds to the RNA fragments containing the m6A site. Subsequently, the immunoprecipitated RNA fragments (IP) and the unbound RNA fragments (Input) were subjected to library construction using the Ovation SoLo RNA-Seq System Core Kit (NuGEN, USA). The Illumina NovaSeq 6000 platform (Illumina, USA) was used to sequence the resulting libraries in PE150 sequencing mode.