GSM8973657: eWAT_cdna_ctrl_6; Mus musculus; RNA-Seq
All tissues were dissected, washed with PBS and weighed. They were snap-frozen in liquid nitrogen and stored at -80C. Whole frozen eWAT samples were homogenised in 1 ml TRIsure (Bioline) per animal using a tabletop homogeniser (FastPrep-24 5G, MP Biomedicals). Total RNA was isolated using EconoSpin All-In-One Mini Spin Columns (EconoSpin 1920-250). Poly-A+ RNA was iolated using the NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490) following the manufacturer's protocol. Libraries were prepared from 100 ng poly-A+ RNA using the SQK-DCS109 direct cDNA sequencing kit and the EXP-NBD104 barcoding kit according to the manufacturer's protocol.