GSM8835607: uSpyCLIP-seq of dRfxCas13d TP53g1, UV crosslinking, rep3; Homo sapiens; OTHER
The crosslinked cell pellets were resuspended with lysis buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 0.1% SDS, 0.5% sodium deoxycholate, 1% Triton X-100, 1×Protease Inhibitor Cocktail) using 1 ml per 10 million cells and lysed on ice for 10 min. RNA was rationally fragmented using RNase I. uSpyCLIP libraries were constructed using the uSpyCLIP method developed in-house