GSM8973831: PSS008 ISX9-2 for Fig. S5K; Homo sapiens; OTHER
CUT&Tag was performed using the CUT&Tag-IT™ Assay Kit (Active Motif, 53160) with H3K27ac antibody (Abcam, ab4729). Briefly, cells after 6 days' treatment were first incubated with concanavalin A beads and primary antibodies followed by secondary antibody. Then the chromatin is digested by tagmentation using the protein A-Tn5 (pA-Tn5) transposome enzyme. After DNA purification and PCR amplification with adaptors, samples were sequenced on Illumina NextSeq2000 CUT&Tag was performed using the CUT&Tag-IT™ Assay Kit (Active Motif, 53160) with H3K27ac antibody (Abcam, ab4729). Briefly, cells after 6 days' treatment were first incubated with concanavalin A beads and primary antibodies followed by secondary antibody. Then the chromatin is digested by tagmentation using the protein A-Tn5 (pA-Tn5) transposome enzyme. After DNA purification and PCR amplification with adaptors, samples were sequenced on Illumina NextSeq2000