Nanopore sequencing of Alphaherpes virus 1 from an hunting dog (Italy)
Two portions of dog brain tissue were collected: one was used for DNA extraction for Real-Time PCR, while the other was used to infect a Vero cell line. After cell line DNA extraction, sequencing library preparation was performed using the Native Barcoding Kit 24 V14, according to the standard procedures. Sequencing was carried out on a Mk1C device, with a R10.4.1 flow cell (FLO-MIN114), with Guppy v6.5 base caller and "hac" calling mode.