GSM8698941: NSN oocyte_oe-mCherry_RNA-seq_rep2; Mus musculus; RNA-Seq
As previously described by Crosetto et al. (2015), RNA was extracted from lysed cells and precipitated overnight. RNA was then reverse-transcribed into cDNA using Thermo Fisher's SuperScript II Reverse Transcriptase (Cat No. 18064014). cDNA amplification was performed using KAPA HiFi HotStart DNA Polymerase, followed by library construction with the Vazyme TD503 kit.