RNA-Seq of artificial infant gut microbiome consortium, all microorganisms
Total RNA was first isolated from the samples using the RNeasy Kit (Qiagen). After quality verification, the high abundance ribosomal RNA (rRNA) was depleted using the QIAseq stranded total rRNA kit (Qiagen). The sequencing library was then prepared from the remaining non-ribosomal RNA species using the QIAseq Stranded RNA Library kit (Qiagen), resulting in a strand-specific Total RNA-Seq library. The final library was sequenced on an Illumina NextSeq 500 instrument.