GSM8870469: Endometrium, d16, bacteria infused-cow7; Bos taurus; RNA-Seq
RNA was extracted using the RNeasy Mini kit (Qiagen) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by a second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library. The non-directional library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification.