GSM8733549: Cux2ERT,M,Saline,1week,rep4,b2; Mus musculus; RNA-Seq
RNA purification from RiboTag mice were modified from previously published protocols (see methods for details). RNA purification was performed using a RNeasy Mini kit following the manufacturer's instructions. Samples with RNA integrity number (RIN) values >7 were used for library prep. 50 ng of RNA was used for library preparation with TruSeq stranded RNA library prep kit with poly-A enrichment. The libraries were sequenced 150bp paired-end on a NovaSeq 6000 platform for DOI samples, and X Plus for psilocybin samples, at 200 million reads per sample.