RNA-seq  of Intergenic eccDNA transfected PC-3; Human; adult male; Replicate2
Intergenic eccDNA were transfected into PC-3 cells with the same operation for CCK-8 assay and scratch healing assay. After 48 hours of transfection, RNA from transfected cells were extracted with two replicates. Poly-T oligos enriched mRNA was subjected to RNA-seq library construction as described previously. All libraries were sequenced on the Illumina NovaSeq X Plus platform with paired-end 150 bp.