Whole Genome sequencing of a Listeria monocytogenes from a chicken.
Genomic DNA was extracted from isolated colonies using the PureLink Genomic DNA Mini Kit (ThermoFisher Scientific, Waltham, MA, USA) according to the manufacturers instructions. Sequencing was performed using two complementary platforms: Illumina NextSeq 2000 for high-accuracy short reads and the Oxford Nanopore Technologies (ONT) GridION for long-read data. Libraries were prepared using the Illumina DNA Prep kit (Illumina, San Diego, CA, USA) and the ONT Ligation Sequencing Kit V14 (SQK-LSK114), respectively.