GSM8951081: IFNAR-/-IAV AMs2; Mus musculus; RNA-Seq
A total of 400,000 purified AMs per replicate were pelleted and lysed in TRIzol reagent (Thermo Fisher, Waltham, MA) and cryopreserved at -80 °C, followed by RNA extraction. RNA libraries for Rna-seq were prepared uding NEBNext UltraTM RNA Library Prep Kit (New England Biolabs, Ipswich, MA) following manufacturer's recommendations.