GSM9067988: CBDIL12-CART | 011 | Full ROI; Mus musculus; OTHER
FFPE tissue blocks were sectioned by UCLA Translational Pathology Core Laboratory (TPCL). RNA was extracted from mouse FFPE samples with Qiagen RNeasy FFPE Kit. Slides were stained using NanoString GeoMx (v1.0) Mouse NGS Whole Transcriptome Atlas RNApanel according to manufacturer instructions. ROIs were selected based on Anti-PanCK, Anti-CD45, and DNA staining as morphological markers. Oligo barcodes were collected from ROI into microtiter plates by the GeoMx® Digital Spatial Profiler (DSP) platform. GeoMx Seq Code Pack were used for multiplexing samples in one lane. Sequencing was performed on Illumina NovaSeq X Plus for PE 2x50 runs.