RNAseq of Toxoplasma gondii: TgHDAC2 knockout, complemented and control strains
Total RNA was isolated from syringe released Toxoplasma gondii using RNAeasy kit following manufacture's instructions (Qiagen). The RNA was quantified using Qubit and 1ug of RNA was used for library construction, the Illumina Stranded mRNA Prep Ligation Kit was used according to the manufacturers instructions (Illumina). The libraries were assessed for size distribution and quality using Agilent Bioanalyzer. Only high-quality libraries with the expected size range were used for sequencing on Illumina Hiseq 2500.