GSM9325834: magzc2; Rattus norvegicus; RNA-Seq
Total RNA was isolated from frozen anastomotic tissue using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. RNA integrity and concentration were assessed using NanoDrop One (Thermo Fisher Scientific). RNA samples were previously sequenced by Annoroad Gene Technology Co., Ltd. (Beijing, China). Sequencing libraries were constructed using NEBNext Ultra RNA Library Prep Kit (New England Biolabs, USA) and sequenced on the DNBSEQ-T7 (MGI Tech Co., Ltd.) platform. The data uploaded here represent normalized FPKM expression matrices derived from that sequencing.