Genome Sequence of Saccharomyces cerevisiae NCFS1
DNA was extracted using Zymo Research YeaStar Genomic DNA Kit following manufacturers protocols. Library preparation was performed using the SMRTbell Express Template Prep Kit 2.0 (PacBio), and sequencing was carried out on a PacBio Sequel IIe platform.High-molecular-weight genomic DNA was sheared to a target size of 1020 kb, followed by end-repair and ligation of barcoded SMRTbell adapters.