DNAseq of Anopheles stephensi strain SDA500 driver line
DNA was extracted using PureLink Genomic DNA Mini Kit following manufacturers instructions (ThermoFisher Scientific, Waltham, MA, USA). Genomic DNA was quantified using the Qubit 4.0 Fluorometer (ThermoFisher Scientific, Waltham, MA, USA). NEBNext Ultra II DNA Library Prep Kit for Illumina was used for library preparation, following the manufacturers recommendations. Briefly, the genomic DNA was fragmented by acoustic shearing with a Covaris S220 instrument. Fragmented DNA was cleaned and end-repaired. Adapters were ligated after adenylation of the 3ends, followed by enrichment by limited cycle PCR. DNA libraries were quantified using Qubit 4.0 Fluorometer. The DNA libraries were also quantified by real-time PCR (Applied Biosystems, Carlsbad, CA, USA).The sequencing libraries were clustered onto a 25B flowcell on the Illumina NovaSeq X Plus instrument according to the manufacturers instructions. The samples were sequenced using a 2x150 paired-end (PE) configuration, targeting ~1 Gb/sample. Sample extraction, quality assessment, DNA library preparation, sequencing, and initial bioinformatics analysis were conducted at Azenta Life Sciences (South Plainfield, NJ, USA).