GSM9837154: GROseq_NHA_RA_24h_rep2; Homo sapiens; OTHER
RNA was extracted using TRIzol LS reagent (Invitrogen) according to the manufacturer's instructions and recovered by ethanol precipitation. Eluted RNA was quantified by Qubit and used for strand-specific library construction using the NEBNext Ultra II Directional RNA Library Prep kit (New England Biolabs). Libraries were sequenced on the Illumina NextSeq 500 platform.