Illumina HiSeq 2500 paired-end Sequencing of KAS22022614 (KAE22247650)
Development of drought-associated SNP markers in Populus davidiana
The total genomic DNA was extracted through nuclei lysis using the CTAB method [13]. Following standard Illumina whole-genome shotgun WGS sequencing protocol, a genomic library with an insertion size of 300bp was constructed for paired-end sequence reads, and three libraries were constructed with insertion sizes of 3k, 5k, and 300bp-10kb for mate-pair sequence reads. Raw sequence reads were generated by Illumina HiSeq 2500 platform