GSM8698051: AFHTN3; Homo sapiens; RNA-Seq
Peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll-Paque PLUS (density 1.077 g/mL) gradient centrifugation. Single-cell RNA libraries were prepared using the 10X Genomics Chromium Controller and Single Cell 3' V3.1 Reagent Kits. PBMCs were concentrated to 1000 cells/μL and loaded into the Chromium chip to generate single-cell Gel Bead-In-Emulsions (GEMs). After reverse transcription, the cDNA was amplified, fragmented, and ligated with adapters. Index PCR was performed, and the final libraries were quantified using Qubit and analyzed for size distribution with a Bioanalyzer 2200. Libraries were sequenced using an Illumina sequencer with a 150 bp paired-end run.