GSM9659161: Stool from Gpr15-/- and Gpr15l+/+ littermate after weaning_9; Mus musculus; OTHER
Fecal DNA was extracted using the QIAamp DNA Stool Mini Kit (Qiagen) according to the manufacturer's instructions. The V4 regions of the 16S ribosomal RNA genes were amplified using 10 ng of bacterial template DNA with region-specific primers (515F forward primer: 5′-GTGYCAGCMGCCGCGGTAA−3′; 806R reverse primer: 5′-GGACTACNVGGGTWTCTAAT−3′), containing 12 bp barcodes on the forward primer (https://earthmicrobiome.org/protocols-and-standards/16s/), in a reaction consisting of 25 PCR cycles using the NEBNext Q5 Hot Start Hifi PCR Master Mix (New England Biolabs). PCR products were purified with AMPure XP Beads (Beckmann Coulter), pooled in equimolar ratios and analysed by 2 × 151 paired-end sequencing on an Illumina MiSeq device.