vaginal sample of Estrogen group for 15 days
Total microbial genomic DNA of vaginal samples was extracted using the E.Z.N.A. Soil DNA Kit (Omega Bio-Tek, Norcross, GA, USA). The extracted microbial DNA was processed to construct metagenomic sequencing libraries with 400 bp insert sizes with NEXTFLEX Rapid DNA-Seq Kit (BIOO Scientific, Austin, TX, USA).