IsoSeq of Pleurodeles waltl spleen transcriptome
PacBio Isoseq data generated from female adult brain tissue from Pleurodeles waltl. Tissue was harvested from the spleen of a female adult P. waltl. Tissue was immediately frozen on dry ice and stored at -80C. Approximately 25 mg of brain tissue was then used for tissue pulverization in liquid nitrogen. Tissue powder was used for RNA extraction using Total RNA Purification Kit (Cat. 17200 from Norgen Biotek) as per manufacturer's recommendation. Genomic DNA was removed via on column DNA removal using Norgen RNAse free DNAase kit (Cat. 25710). RNA quality was analyzed via bioanalyzer and had a RIN value of 9.8. For IsoSeq, cDNA was synthesized using SMARTer PCR cDNA (Clonetech, CA). One IsoSeq library was prepared. Sequencing was performed on a single SMRT cell using PacBio Sequel II.