ST-seq of mice spinal cords: received hiPSC-derived organoids
Spinal cord samples were cryosectioned (10 m) and prepared for spatial transcriptomics using the 10x Genomics Visium platform. Sample quality (morphology & RNA integrity  RIN 7.9-8.8) was confirmed prior to analysis. Each slide contained approximately 5,000 spots (55 m diameter, 100 m spacing) for capturing RNA. Permeation times were optimized for each group (6-18 min) using the Visium Tissue Optimization Kit. Libraries were generated with the Visium Spatial Gene Expression Kit and sequenced on an Illumina NextSeq.