RNA-seq WT replicate 4 UV treated
We collected WT and fas1 control leaves and leaves exposed to UV-C radiation (6 kJ/ m), subsequently incubated in complete darkness for 2 h in triplicates (100 mg each) and frozen at dry ice. The samples were then stored at -80 C until the RNA extraction using kit RNeasy Plant Minikit (Qiagen), according to the manufacturer's instructions. RNA integrity was checked on the Fragment Analyzer using RNA Kit 15 nt (Agilent Technologies). 250 ng of total RNA was used as input for library preparation using QuantSeq 3 mRNA-Seq FWD with UDI 12 nt Kit (v.2) (Lexogen) in combination with UMI Second Strand Synthesis Module for QuantSeq FWD. Quality control for library quantity and size distribution was done using QuantiFluor dsDNA System (Promega) and High Sensitivity NGS Fragment Analysis Kit (Agilent Technologies). Final library pool was sequenced on NextSeq 500 using High Output Kit v2.5 75 Cycles (Illumina).