GSM8768982: 2D_hIgG_48h_1; Homo sapiens; RNA-Seq
RNA was isolated following the manufacturer's protocol for the RNeasy Mini Kit (Qiagen, #74104), and residual DNA was removed using the RNase-Free DNase Set (Qiagen, #79254). RNA quality was assessed using the NanoDrop™ 2000 spectrophotometer (Thermo Fisher Scientific, #ND-2000), and samples were stored at −20°C until sequencing. Illumina TruSeq Stranded Total RNA Library Prep Kit with RiboZero Plus rRNA depletion.