RNAseq of CD34 derived neutrophils
RNA was extracted using the MiniRNA Isolation Kit (Thermo Fisher Scientific). The quality between was evaluated using the Fragment Analyzer. RNA that passed the quality control (QC) was then used to generate libraries using the NEBNext Library Ultra Low Input Library Preparation. The manufacturers recommendations were followed, and the libraries were sequenced on an Illumina NovaSeq6000 sequencer. All sequencing data were performed in three biological replicates, having a total depth of 15 million reads per reads. The reads were conducted at 2  150 base pair (bp) of length.