GSM9617712: MC38_CD45pos_scRNAseq_hashing_Lib2_GEX; Mus musculus; RNA-Seq
Tumors were excised from MC38 tumor-bearing mice and mechanically dissociated into single-cell suspensions. CD45+ immune cells were isolated by fluorescence-activated cell sorting (FACS). Single-cell libraries were generated using the 10x Genomics Chromium Single Cell 3' Gene Expression platform according to the manufacturer's protocol. Samples from multiple mice were multiplexed using cell hashing antibodies before loading onto the Chromium controller.