ATAC-seq of white birch: leaves
Total nuclei were isolated from fresh leaves of  Betula platyphylla. In brief, leaf tissues were homogenized in cold nuclei isolation buffer. The crude lysate was filtered and centrifuged to pellet nuclei. The isolated nuclei were then subjected to tagmentation using the Tn5 transposase, which simultaneously fragments and tags the genome at regions of open chromatin. The tagmented DNA was purified using a column-based method. Subsequently, the fragmented DNA was amplified by PCR with index primers to construct the sequencing library. The final library was quantified and assessed for quality before being subjected to paired-end 150 bp sequencing (PE150) on an Illumina platform.