Transposon insertion site sequencing of a pooled mutant library of Pseudomonas chlororaphis ATCC 9446
Barcode-enriched Tn-Seq libraries were prepared and sequenced by Fasteris Corp. (Switzerland) using their in-house Tn-Seq v6 workflow, which enriches transposongenome junctions prior to sequencing. Libraries were run on an Illumina MiSeq with single-end 250-bp reads.