GSM8736105: CC5325, Chlamydomonas reinhardtii; Chlamydomonas reinhardtii; RNA-Seq
Total RNA was extracted using Trizol (Sangon) according to the manufacturer's instructions. rRNA was depleted from total RNA using the Ribo-off rRNA Depletion Kit (Vazyme). RNA library construction was then performed using the VAHTS Universal V10 RNA-seq Library Prep Kit for Illumina (Vazyme), following the manufacturer's instructions. The libraries were assessed using the Agilent 2100 Bioanalyzer and sequenced on the Illumina platform using 150 bp paired-end sequencing.