GSM8733676: granulosa cells, Control, 12h, Input; Homo sapiens; ChIP-Seq
Chromatin was extracted from cells fixed with 1.1% formaldehyde and fragmented using sonication. Libraries from purified DNA from ChIP and input samples were prepared with the UltraLow System V2 DNA-Seq Library Preparation Kit (Tecan; Durham, NC, USA). The libraries were pooled and quantitated by qPCR and sequenced on one 10B lane for 101 cycles from one end of the fragments on a NovaSeq X Plus with V1.0 sequenced kits (Illumina).