WGS of mead yeast
Genomic DNA extraction was performed according to the protocol described by Hanna and Xiao (2006), and DNA concentrations were standardized to 100 ng/L for all isolates. Library preparation was performed using tagmentation with the Nextera DNA Flex Library Prep kit (Illumina, San Diego, CA, USA) according to the manufacturers protocol. Sequencing was performed using 150 bp paired-end reads on an Illumina NextSeq 500 system, with approximately 5080 coverage of the nuclear genome. In the case of Novogene, sequencing was performed using paired-end 150 bp technology on an Illumina NovaSeq X Plus system. During library preparation, genomic DNA was fragmented to an average size of 350 bp, and size selection was carried out using sample purification beads.