GSM9216962: Lag3-/-; 5xFAD+, 3 month, female, rep3; Mus musculus; RNA-Seq
Total RNA from FACS purified CD11b+; CD45low hippocampal microglia was isolated using RNeasy Plus Mini Kit (Qiagen) and stored at -80°C. cDNA was generated using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara). The cDNA libraries were prepared using the Nextera XT DNA Library Prep Kit and Nextera XT index Kit (Illumina) and sequenced by the NIEHS Epigenomics and DNA Sequencing Core Facility using a NextSeq 500 NGS system (Illumina).