GSM9393820: Day 19 time-stamped TCL-1 mouse 91, FACS sorted Tom+ B cells, time point 2; Mus musculus; OTHER
FACS sorted B cells were lysed in RNAzol (Sigma-Aldrich) or DNA/RNA shield (Zymo Research) and total RNA was extracted with Direct-zol RNA MiniPrep Kit (Zymo Research) according to manufacturer's instructions. IgHM transcripts were captured via their constant region and cDNA was generated via template switch reverse transcription using SMARTScribe Reverse Transcriptase (Takara Bio). cDNA libraries were PCR amplified with Q5 High-Fidelity DNA Polymerase, purified with AMPure XP Reagent and quantified with Qubit Fluorometer (dsDNA HS Assay Kit). Up to 2.5 ng cDNA library was indexed with Nextera XT Index Kit v2 SetA (Illumina). Final cDNA library  quality was assessed with Agilent 2100 Bioanalyzer (High Sensitivity DNA Kit).