Bulk_injured_ATAC
Motor cortices were isolated from mouse pups at P0,P4, P7, and Adult mice underwent intra-cortical surgery. Tissue (20-30 mg) was minced and placed into ice-cold PBS. Nuclei extraction was done following the protocol mentioned above. Nuclei were counted, and approximately 50,000 to 100,000 nuclei per sample were used for the assay. The pellet dissolved in the Nuclear Suspension Buffer was immediately undergone  tagmentation by adding transposase enzyme, where transposase enzymes inserted sequencing adapters into accessible regions of the chromatin using the ATAC -Seq kit (Active motif-53150), following the manufacturers manual protocol. The tagmentation was carried out by incubating the nuclei with a Tagmentation Master Mix at 37C for 30 minutes. Following tagmentation, the DNA was purified using a DNA purification column. The purified DNA was eluted and subjected to PCR amplification using indexed primers compatible with Illumina sequencing. Post-PCR, a final purification step was performed using SPRI beads, and the libraries were quantified for sequencing. DNA size distribution was assessed with  TapeStation before loading the libraries onto the Illumina sequencing platform.