GSM8869809: SparkT replicate 2 scATACseq; Mus musculus; ATAC-seq
Nuclei of sorted CD5lowCD11ahigh and CD5highCD11alow T cell subpopulations from OT-1 Rag1-/- mice were isolated following the nuclei isolation protocol for Single Cell ATAC Sequencing (Low Cell Input) provided from 10x Genomics. Cell lysis was performed for 5 min on ice. Nuclei concentration was quantified using Trypan Blue and a Countess II FL Automated Cell Counter. scATACseq libraries were performed according to 10X Genomics guidelines