Short-read WGS of Klebsiella michiganensis RSU-K5
Total DNA was extracted from an overnight culture obtained from a single colony of strain RSU-K5 using Proteinase K/SDS treatment and Genomic DNA Clean & Concentrator-10 Kit (Zymo Research). Extracted DNA (~1 microgram) was then used as input to prepare Illumina MiSeq-compatible DNA libraries for 250 bp paired-end read sequencing using the QIAseq FX DNA Library Kit as per the manufacturer's instructions (using adapter #22 from QIAseq UDI Y-Adapter Kit 24 for this particular library) up to the PCR step (No PCR was employed in this case). The resultant library was sequenced on the Illumina MiSeq system as one of the several individually barcoded pooled libraries using 500-cycle MiSeq Reagent Kit v2. Demultiplexing was carried out using bcl2fastq based on the used primer sequences and demultiplexed reads were trimmed using fastp.