RNA-seq of equine endometrium glandular epithelium Day 8 after ovulation
RNA-Seq library preparation starting from 2.5 ng total RNA and following the instructions of the Ovation Solo Single Cell RNA Seq System kit (NuGen Technologies, San Carlos, USA). Each of the 24 libraries was individually barcoded for multiplexing (1 pool of 12 LE and 12 GE samples). Sequencing of the library pool was performed at the Functional Genomic Center Zurich on one high-output flow cell on an Illumina NextSeq 500 instrument with 150 bp single-end reads and the 16 bp index read (8 bp library barcode and 8 bp unique molecular identifier, UMI).