D2_T13 (CRX793871; CRA012647)
Total RNA was extracted from each ovarian sample by Trizol kit (Invitrogen, Carlsbad, CA,USA), and RNA integrity and genomic DNA contamination were examined by agarose gel electrophoresis. The cDNA library was constructed according to the mRNA Library Preparation Kit (#E7530, New England Biolabs) , including mRNA isolation and interruption, cDNA double-strand synthesis and purification, cDNA end repair, addition of polyA tails and ligation to Illumina sequencing adapters, screening of cDNA fragments around 200 bp, PCR amplification and purification of their products.