zQ Placebo Cortex 2
RNA extraction, library preparation, sequencing and initial data processing were performed by Lexogen NGS Services, Lexogen GmbH, Austria. In brief, RNA was isolated using Lexogens SPLIT RNA Extraction Kit. Samples were characterized by UV-Vis spectrophotometry (Nanodrop2000c, Thermo Fisher) and RNA integrity was assessed on a Fragment Analyzer System using the DNF-471 RNA Kit (15 nt) (Agilent). The libraries were constructed using Lexogens QuantSeq 3' mRNA-Seq Library Prep Kit FWD V2. Prepared libraries were quality controlled on a Fragment Analyzer System using the DNF-474 using the HS-DNA kit (1-6000 bp) (Agilent). cDNA libraries were sequenced with an Illumina NovaSeq X platform using 100nt single end read length, with 5 million reads on average per sample.