GSM8733671: granulosa cells, hCG, 12h, rep 4; Homo sapiens; RNA-Seq
Total RNA was isolated from the cultured cells using a RNeasy mini kit (Qiagen) and eluted in 40 ul of nuclease-free water. Libraries for RNA-Seq were prepared using the TruSeq Stranded mRNA Library Prep Kit (Illumina). The libraries were quantitated by qPCR and sequenced on a single lane for 101 cycles from one end of the fragments on a NovaSeq 6000 with a NovaSeq S2 reagent kit (Illumina).