16S rRNA amplicon sequencing of Mus musculus: feces from 11-month-old Wild-type female mice on high-fat  diet
Total DNA was extracted from mouse fecal samples using the QIAamp PowerFecal Pro DNA Kit (Qiagen). The V4 region of the bacterial 16S rRNA gene was amplified using barcoded primers 515F/806R. PCR products were purified, quantified, pooled in equimolar concentrations, and sequenced on the Illumina MiSeq platform using paired-end sequencing.