Subject ND568 Monocytes ChIP-seq of H3K4me3
Peripheral blood mononuclear cells (PBMC) and monocytes were purified. Cells were fixed, lysed and sonicated according to the ChIP-IT High Sensitivity kit instructions (Active Motif). Libraries were prepared using NEBNext Ultra II DNA Library Kit for Illumina. Flow cytometry was used to ensure purity of the cell type population.