GSM9116635: Combined treatment; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol Reagent and BCP Reagent (Invitrogen) according to the manufacturer's protocol. Poly(A)+ RNA was enriched from total RNA using oligo(dT) selection, followed by RNA fragmentation, first and second strand cDNA synthesis, end repair, adapter ligation, and PCR amplification. Libraries were prepared using the Universal Plus mRNA-Seq with NuQuant kit (TECAN) according to the manufacturer's instructions.