Sequel sinlge-end Sequencing of KAS22022817 (KAE22249750)
Construction of reference genome of Pinus densiflora
To construct the isoform sequencing libraries, the pooled RNA was synthesized to cDNA using the SMARTer PCR cDNA Synthesis Kit Clontech 634925, followed by size fractionation using the BluePippin Size Selection System. Three SMRTbell ibraries 1~2, 2~3 and >3 kb in length were constructed with a Pacific Biosciences SMRTbell Template Prep Kit 1.0 according to the manufacturer's protocol. The libraries were then sequenced using 2 SMRT cells per library Pacific Biosciences, Sequel??SMRT Cell 1M v2 and sequencing kit Sequel Sequencing Kit 2.1