GSM9181145: sample 2 of liver constituent cells from a wild-type male mouse after pIVCL, treated with DMSO; Mus musculus; RNA-Seq
After liver perfusion with pronase (#107433; Sigma‒Aldrich) and collagenase (#C5138; Sigma‒Aldrich), the cell suspension was conditioned to be 90% liver nonparenchymal cells and 10% hepatocytes, and dead cells and erythrocytes were removed via density gradient centrifugation using Percoll PLUS (GE17-5445-02; Cytiva). The percentage of viable cells in the cell suspension was greater than 70%, and approximately 10,000 viable cells were used for library preparation. For the construction of the scRNA-seq library, a cDNA Kit and Whole Transcriptome Analysis (WTA) Amplification Kit (Becton Dickinson and Co., Franklin Lakes, NJ) were used according to the manufacturer's instructions. Rhapsody WTA libraries were sequenced on the DNBSEQ-G400RS platform (MGI Tech).