GSM8641803: ChIPseq_CTCF_Dac1J129sv_E11.5_FLHL_Rep2; Mus musculus; ChIP-Seq
ChIP experiments were performed following the protocol described in (Distinct global shifts in genomic binding profiles of limb malformation-associated HOXD13 mutations. Ibrahim et al.,Genome Research 2013). Snap frozen samples were first digested and chromatin was sonicated with a Diagenode Bioruptor (45 cycles- 30 sec pulse, 30 sec pause, HI power). 25-35 ug of chromatin was used in each immunoprecipitation with 1ug of the following specific antibodies: anti-H3K4me3, Millipore, cat. No. 07-473; anti-H3K4me1, Diagenode, cat. No. C15410037; anti-H3K27ac, Diagenode, cat. No. C15410174; anti-CTCF, Active Motif, cat. No. 61311; anti-Rad21, abcam, cat.No. ab992. Libraries were prepared using the Nextera adaptors and sequenced.