RNA-seq of Raji cells expressing either IL-13Rα1 or IL-13Rα1-LOR1a and treated with IL-13 or not
RNA-seq of Raji cells expressing either IL-13Rα1 or IL-13Rα1-LOR1a and treated with IL-13 or not
Raji cells were grown in, stimulated and collected from culture dishes Cells were grown in RPMI, with 10% heat-inactivated fetal calf serum Cells were treated with 20 ng/ml recombinant IL-13 (Peprotech, Cat #200-13) for 72 hrs RNA from cultured Raji cells was extracted using RNeasy kit (Qiagen, Cat #74104) Library prep was performed with NEBNext Ultra II Directional PolyA mRNA kit (NEB, Cat #E7760)