GSM9617571: Mouse_Sham2; Mus musculus; OTHER
Sham lumbar spinal cord were obtained from mice and snap frozen in liquid nitrogen before embedding in OCT. Embedded tissues were sectioned at 10um before placement within fiducial frames of 6.5mm x 6.5mm capture areas on a Visium Spatial glass slide. These sections then undergo H&E staining (MethanolFixationandHEStaining_RevB), followed by imaging on a Leica DM6B and at 10x magnification. Imaged sections are permeabilized, reverse-transcribed before denaturation. The collected cDNA are further amplified and indexed before sequencing to achieve 75 million reads, which equated to approximately 42,501 reads per spot for the presented data.