GSM8972732: 331R-2D; Biological replicate 1; Homo sapiens; RNA-Seq
Total RNA was extracted with the Maxwell RSC SimplyRNA Cells kit (Promega).  QC was checked with the Agilent Tapestation high sensitivity RNA kit, and the Qubit high sensitivity RNA assay. 300ng of total RNA was used as input for polyA enrichment with the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB), and the MGIEasy RNA Directional Library Prep Set (MGI) following manufacturer's instructions.  Library QC was checked with the Agilent Tapestation high sensitivity d1000 kit.  Single stranded circular libraries and DNA nanoballs were prepared following manufacturer's instructions prior to DNBSEQ-G50 sequencing (PE100).