AMPLICON of metagenomes: sediment sample
surface sediment from lake littoral -> mixing with phosphate buffer to release DNA -> centrifugation and capture of DNA from supernatant on filters -> DNA extraction on columns -> PCR with BF3/BR2 primers -> indexing PCR with fusion BF3/BR2 primers -> sequencing, demultiplexing, primer trimming, securing uniform orientation (sequences in R1 file start from the BF3 end)