PacBio sequencing of C.elegans MA line 459 (mixed stage)
.  Worms were washed from the plates, double-rinsed in M9 buffer, and pelleted for DNA extraction.  DNA was extracted from each sample with the Quiagen Gentra Puregene kit, following the manufacturer's instructions.  Extracted DNA samples were sent to the University of Maryland genomics core facility for library preparation and sequencing on the Pacific Biosciences Sequel platform