GSM8660263: Infect_with_InfectKO; Mus musculus; OTHER
The Gene Expression Kit (BMKMANU, #ST03002) and Tissue Optimization Kit (BMKMANU, #ST03003) were used as per the manufacturer's instructions. Tissue was embedded in OCT on dry ice and stored at −80°C. Sections were cut to 10 μm thickness, transferred to the Gene Expression Slide, and stained with H&E. Imaging was performed using a Pannoramic MIDI microscope, and tile scans of the entire array were acquired Libraries were constructed using the BMKMANU Library Construction Kit (BMKMANU, #ST03002-34). Truseq Illumina libraries were prepared and sequenced on a NovaSeq with a depth of 150,000 read-pairs per spatial spot. Sequencing followed the protocol: Read 1 (28 cycles), i7 index (10 cycles), i5 index (10 cycles), and Read 2 (50 cycles)