GSM9068419: GFP-RIP_Tgs1-GFP_abp1DDE_eas1∆mug174-1_BioRep1a; Schizosaccharomyces pombe; RIP-Seq
Immunoprecipitation of epitope tagged protein, Proteinase treatment to remove protein; DNAse treatment to remove DNA and acid phenol-chloroform extraction to isolate associated RNA followed by ethanol precipitation For RIP Seq-Sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina according to the manufacturer's protocol and analyzed using an Agilent 4200 TapeStation system (Agilent). RNA coimmunoprecipitated with epitope tagged proteins were used for library preparation.