Illumina Short-read NGS of a de novo RNA hairpin library using TInDel Assembly
Illumina Short-read NGS of a de novo RNA hairpin library using TInDel Assembly
The library was amplified via PCR using OneTaq DNA polymerase (New England Biolabs) with the following conditions: 30 sec at 94°C; 15 cycles of 20 sec at 94°C, 30 sec at 54°C, 30 sec at 68°C; 5 min at 68°C. Primers can be found in the manuscript Supplementary Table 2. The PCR product was extracted from a 4% agarose gel using the Monarch DNA Gel Extraction kit from New England Biolabs. The purified PCR product was submitted as is without adapters.