RNA-seq of Harmonia axyridis DMT-L-3 head
Total RNA from head of Harmonia axyridis. mRNA was purified using poly-T oligo-attached magnetic beads. cDNA synthesis was performed using random hexamer primer and M-MuLV Reverse Transcriptase. The library fragments were purified with AMPure XP system and cDNA fragments of 370-420 bp were selected. PCR amplification was performed. Sequencing was performed on Illumina NovaSeq platform with 152 bp paired-end reads.