ITS sequencing of tomato microbiome
DNA was extracted from 0.25 mg of phyllosphere, endosphere, and rhizosphere samples (48 in total, representing two soil origins, healthy and diseased plants, and four replicates) using the DNeasy PowerSoil Pro Kit (Qiagen, Germany) following the manufacturer's instructions. Amplicon libraries targeting the ITS region were generated with primers ITS1F (5-CTTGGTCATTTAGAGGAAGTAA-3 and ITS2 (5-GCTGCGTTCTTCATCGATGC-3).