scRNA-Seq of human: adult female endometrium
The human endometrium was rinsed with ice-cold DPBS (Merck Millipore, Cat. D8537) and chopped using scalpels into small pieces and enzymatically digested in 5 mL DPBS containing 1 mg/mL collagenase IV (Worthington, Cat. LS0004188). The supernatant was diluted with DPBS and passed through a 40 m cell sieve (Biosharp, Cat. BS-40-CS). The flow-through was centrifuged and resuspended in 1 mL of red blood cell lysis buffer for 2 minutes. The cells were collected by spinning down at 500 rpm for 5 minutes and re-suspended in DPBS a for subsequent 10XscRNA sequencing.