16s rRNA mus musculus
Bacterial DNA was extracted using the PowerSoil DNA Isolation Kit (MOBIO laboratories, Qiagen, Germany) according to the manufacturer's instructions. DNA was quantified with a Tecan Infinite M200 Pro (Tecan, Austria) and integrity assessed by agarose gel electrophoresis (1%). PCR amplification of the v3-v4 region, library preparation, and amplicon sequencing (Illumina MiSeq platform) were performed by Omega Bioservices, Norcross, USA.