ITS of Helicoverpa armigera: whole gut
Libraries were prepared using amplification reactions in a qPCR equipment to control cycles and limit chimera formation. Amplicons were quantified by reading qPCR fluorescence and were grouped by molarity. The libraries were then filtered using Select-a-Size DNA Clean & Concentrator (Zymo Research, Irvine, CA), following the manufacturers instructions, and quantified with TapeStation (Agilent Technologies, Santa Clara, CA)  and Qubit (Thermo Fisher Scientific, Waltham, WA).