HSPCs_HE3 (CRX1195493; CRA019121)
The amplified double-stranded cDNA is interrupted and spliced by Tn5 transposase, amplified by PCR and purified by magnetic beads to obtain the desired sequencing library. The constructed library is qualified by Agilent 2100 Bioanalyzer and Q-PCR for sequencing. submitted_instrument_model: BGISEQ-100