Illumina NovaSeq 6000 paired end sequencing of SAMD00423965
1) DNA fragmentation: Covaris E220, Forcused-ultrasonicator. 2)Adapter ligation: KAPA Hyperprep Kit PCR-free: Kapa Biosystems (REF: KK8503) and Truseq DNA sgl Index: Illumina (REF: 20015960 and 20015961). 3) Post-ligation Clean up: Clean NGS: GC Biotech (REF: CNGS-0050). 4) Bisulfite conversion : EZ DNA Methylation-Gold Kit: ZYMO Research (REF: D5005). 5) Library amplification: KAPA HiFi Hotstart Uracil+ Kit: Kapa Biosystems (REF: KK2801) and KAPA Library Amplification Primer Mix: Kapa Biosystems (REF: KK2623). 6) Post-amplification Clean up: Clean NGS: GC Biotech (REF: CNGS-0050)