GSM9067318: LO_day8; Mus musculus; RNA-Seq
BLOs total RNA Isolation. Discard the culture medium of BLOs, wash twice with PBS, and aspirate BLOs into a 1.5 ml centrifuge tube using a 1 ml pipette tip. After centrifugation at 300× g for 5 min, remove supernatant. Add 500 μl TRIzol Reagent (TR118) to extract total RNA following the product instructions. After total RNA was isolated. Sequencing libraries were prepared according to the VAHTS mRNA-seq v2 Library Prep Kit for Illumina (NR605, Vazyme). Library sequencing used Illumina NovaSeq X Plus Sequence,which provided by ANNOROAD GENE TECHNOLOGY.