GSM9069684: patient40; Homo sapiens; RNA-Seq
Meningiomas were removed, flash frozen in liquid nitrogen, mechanically lysed using a TissueLyser II, and RNA was extracted from the lysed tissue using the Qiagen AllPrep DNA/RNA/miRNA Universal Kit (CAT# 80224). Long-read RNA-seq libraries were constructed using the NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module, PacBio Iso-Seq Express Oligo Kit and SMRTbell express template prep kit 3.0 according to the manufacturer's protocol.