GSM8768508: KO52, p42, UT, R3; Homo sapiens; RNA-Seq
RNA was isolated from 1-2 x 106 cells in 1mL of TRIzol Reagent (ABP Biosciences FP312) following the manufacturer's instructions. For low starting cell number samples, RNA was extracted with Direct-zol RNA MicroPrep kit (Zymo R2060). 1) Oligo dT Selection to enrich the mRNA. 2) RNA fragment and reverse transcription (second-strand cDNA synthesis with dUTP instead of dTTP). 3) End repair, add A and adaptor ligation 4) PCR 5) Single strand separation and cyclization 6) DNA nanoball synthesis