GSM9036088: Mouse cell, cellSp_EE_1, rep 1; Mus musculus; Bisulfite-Seq
Genomic DNA was isolated from mouse liver tissue or Hepa1-6 liver cells with the QIAamp DNA Mini kit (Qiagen), quantified by Qubit fluorometry, fragment size verified on a Fragment Analyzer, and spiked with 0.5 % unmethylated λ-DNA as a bisulfite-conversion control. One µg gDNA was sheared (~250 bp) on a Bioruptor, end-repaired, A-tailed and ligated to 5-mC Illumina adapters; DNA was bisulfite-converted (EZ DNA Methylation-Gold, Zymo), PCR-amplified (8–12 cycles, KAPA HiFi Uracil+), size-selected with AMPure XP beads, QC'd on a Bioanalyzer, quantified by qPCR, and sequenced as 2 × 150 bp reads on an Illumina NovaSeq X Plus.