Nanopore sequencing of Ramulariopsis pseudoglycines DNA: pathogen of cotton
Genomic DNA was prepared for sequencing by first applying a DNA repair and end-prep step, then adapter ligation and clean up using the Ligation Sequencing Kit V14 (SQK-LSK114), New England Biolabs (NEB) kits M6630, E7546, E6056, and Invitrogen AM2616 as specified in the manufacturers protocols. DNA was sequenced using the Oxford Nanopore MinION Mk1C platform and the R10.4.1 Flow Cell (FLO-MIN114)