GSM9067165: HepG2, 0uM 5-azacytidine, rep2, ATAC-seq; Homo sapiens; ATAC-seq
Total RNA was extracted using the RNeasy Mini Kit (Qiagen, 74104) according to manufacturer's protocol. For ATAC-seq, nuclei were extracted and processed according to standard ATAC-seq protocol using Nextera DNA Library Prep Kit. RNA libraries were prepared with the TruSeq Stranded Total RNA Library Prep Gold Kit (Illumina). ATAC-seq libraries were prepared using the Nextera DNA Library Prep Kit (Illumina).