DNA-Seq of Drosophila melanogaster: whole adult male
Genomic DNA was quantified, sheared by Covaris, and prepared using the NEBNext Ultra DNA Library Prep Kit for Illumina, including end-repair, A-tailing, adapter ligation, and limited-cycle PCR enrichment. Libraries were assessed on an Agilent TapeStation and quantified by Qubit and qPCR before sequencing.