MOA-seq A549 Control
DNA concentration was quantified by UV spectroscopy, and digestion patterns were visualized using gel electrophoresis on agarose gels. Libraries were pooled and selected based on MOA-seq light digest criteria as described by Savadel et al., 2021. One to two digests from agarose gels, showing a continuous smear of DNA with fragments in the less than 200 bp size range, were selected for sequencing. Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7103) protocol up to adapter-ligated DNA cleanup without size selection. Pippin size selection was used to select 150-250 bp fragments, which included 120 bp of library adapters. Sequencing was performed using Paired-End 50-base pair Illumina sequencing. All samples were pooled to obtain 80 million read pairs per library.