GSM9696766: HASMC, TCF7L2 IP, rep2; Homo sapiens; ChIP-Seq
Cells were crosslinked with 1% formaldehyde, quenched with glycine, and lysed prior to chromatin preparation. Approximately 2 ng of immunoprecipitated chromatin per sample was used for library preparation with the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, E7645). Sequencing was performed on a NovaSeq X platform to generate 151 bp paired-end reads, with approximately 50 million reads per sample.