16S (V3-V4) of Cnaphalocrocis medinalis
Metagenomic DNA was isolated from third-instar F1 larvae by the method adopted by Zhou et al. (1996). PCR amplification was done using the universal primers 27F (5 AGAGTTTGATCCTGGCTCAG) and 1492R (5 GGTTACCTTGTTACGACTT) (Frank et al., 2008). The amplicon library was prepared with specific primers V3F and V4R spanning the hypervariable V3-V4 region of the 16S rRNA gene. 5ng of amplified product was used for library preparation using the NEBNext Ultra DNA library preparation kit. The library quantification and quality estimation were done in Agilent 2200 TapeStation. The prepared library was sequenced (paired-end sequencing) in the Illumina MiSeq platform.