RNA-seq of PBMCs isolated from patients with various cancers and COVID-19 infection status
RNA-seq of PBMCs isolated from patients with various cancers and COVID-19 infection status
Whole blood was collected and stored at -80Â°C. Samples were incubated at room temperature for 2 hours prior to RNA extraction.  PAXgene Blood RNA Kit (Qiagen, 762174) was used to isolate RNA from blood. The NEBNext Globin and rRNA Depletion Kit (NEB, E7775X) was used to deplete globin and rRNA from 300ng of total RNA. NEBNext Ultra II RNA Library Prep Kit (NEB, E7775L) was used to construct the RNA libraries. HT DNA Extended Range LabChip (Perkin Elmer, 760517) was used to verify the library sizes and concentration, as well as pool to equal concentrations prior to sequencing.