Serum_ND2 (CRX1183907; CRA018899)
Total RNA was isolated using TRIzol reagent (Invitrogen), and rRNA was removed using a Ribo-Zero Gold rRNA Removal Kit (Illumina). The RNA libraries were then prepared from the rRNA-free samples using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) according to the manufacturer's instructions. Three independent biological replicates were carried out for each RNA-seq experiment