RNA-Seq of Oryza sativa : Panicle
Total RNA was isolated from samples using RNAiso Plus reagent according to the manufacturers instructions. RNA quantity and quality were assessed using spectrophotometry and integrity evaluation methods, and only high-quality RNA was used for library preparation. mRNA was enriched from total RNA using poly(A) selection, followed by fragmentation and first- and second-strand cDNA synthesis using random primers. The resulting double-stranded cDNA was end-repaired, A-tailed, and ligated with sequencing adapters. Libraries were amplified by PCR, purified, and quality-checked prior to sequencing. Final libraries were sequenced on an Illumina platform following standard manufacturer protocols.