IsoSeq reads from RNA extracted from multiple P. citri mealybugs with bacteriome removed, first biological replicate
IsoSeq libraries were prepared following the PacBio protocol titled Preparing Iso-Seq libraries using SMRTbell prep kit 3.0 (v02.2022).Pooled libraries underwent the PacBio binding and cleanup steps using the Revio Polymerase kit. Prepared libraries were loaded onto the PacBio Revio instrument. Demultiplexing, quality control, and adapter trimming was performed on instrument via Lima