sclB_20h_Veg_rep-1
Briefly, the RNA-seq library was prepared as follows: The quality of the RNA was defined by RNA integrity number (RIN) by employing the Fragment Analyzer HS Total RNA Kit. The library for the RNA-seq was constructed by using the Illumina Stranded mRNA Prep, Ligation as well as the Illumina RNA UD Indexes Set A, Ligation starting from 300 ng of total RNA. The size selection of the final cDNA library was assessed by employing  the SS NGS Fragment 1- to 6000-bp Kit on the Fragment Analyzer (average 340 bp). The quantification of the cDNA library was measured by the QuantiFluor dsDNASystem (Promega) and lastly the amplification and sequencing of the library was performed by the S4 ow cell NovaSeq6000; paired-end, 300 cycles.