Single-cell transcriptome and T cell receptor profiling of the tuberculin skin test
Single-cell transcriptome and T cell receptor profiling of the tuberculin skin test
Participants received intradermal injection of 0.1 ml 2U tuberculin in the volar aspect of the forearm. At 48 hours, suction blisters were induced at the injection site through the application of negative pressure and blister fluid aspirated 2-4 hours later. Blister cells were recovered after centrifugation of blister fluid. Cells were stained with the TotalSeq-C Human Universal Cocktail v1 (BioLegend). Nucleic acids were extracted following the manufacturer's instructions for the Chromium SingleCell 5' Reagent kit v2 (10x Genomics). Library construction was performed using the Chromium SingleCell 5' Reagent kit v2 (10x Genomics) according to the manufacturer's instructions to generate gene expression, T cell receptor (TCR) VDJ, and surface protein (= feature barcode) libraries.