GSM9069365: DNA_Me_GSC20 mtg483 3hr rep A; Homo sapiens; Bisulfite-Seq
DNA was extracted using the Qiagen AllPrep genomic DNA/RNA extraction kit (Qiagen). Experiments were performed in duplicates (n=2). Genomic DNA was processed using the TruSeq Methyl Capture EPIC Library Prep Kit (Illumina) according to the manufacturer's protocol. Briefly, high-quality DNA (as determined by tapestation analysis) was fragmented via a Covaris S220, followed by end-repair, 3′ adenylation, and ligation of methylation-compatible adapters. The resulting fragments underwent bisulfite conversion to convert unmethylated cytosines to uracils, while preserving methylated cytosines. The libraries were then hybridized to the TruSeq Methyl Capture EPIC probes for targeted enrichment of methylated regions, followed by bead-based capture. Finally, the enriched libraries were PCR-amplified, purified, and assessed for quality with a tapestation prior to sequencing. Sequencing multiplexed and performed on an Illumina NovoSeq 6000.