GSM9696002: QGP-1 MEN1 ChIP RelA rep 2; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde and lysed with cell lysis buffer (10mM Tris-HCL pH 8, 10mM NaCl and 0.2% NP-40), folllowed by nuclear lysis buffer (50mM Tris-HCl pH 8, 10mM EDTA and 1% SDS). Chromatin were sonicated using the Misonix Q500 sonicator (QSonica). Library construction was done using the Novogene DNA Library Prep Kit