22_n1_2 (CRX802797; CRA012806)
The captured mRNA was fragmented and cDNA was synthesized by reverse transcriptase. Then the composite double strands of DNA and RNA were converted into DNA double strands, and the ends of double stranded DNA were filled into flat ends by adding dUTPSolution. Then an A base was added to each end of it so that it could connect with the junction with T base at the end, and the fragment size was screened and purified by magnetic beads. The double strand was digested by UDG enzyme, and then a library with the size of 300bp +/-50bp was formed by PCR.