Amplicon seq of eHAP (pre-treatment)
Cells expressing S.p. dCas9-VP64-p65-Rta (dCas9-VPR) were infected with the Human CRISPR Activation Pooled Library (Calabrese P65-HSF) (Sanson et al., Nat Commun. 2018, PMID: 30575746) at an MOI of 0.2 and 200x coverage. After puromycin selection, cells were fixed with 70% cold ethanol, and genomic DNA was extracted using the Wizard Genomic DNA Purification Kit (Promega) according to the manufacturer's instructions. The extracted genomic DNA was then subjected to gRNA amplification following the protocol from Olivieri and Durocher, STAR Protoc. 2021 (PMID: 33598657).