RNA-seq of mus muscles: adult female spleen
FOXP3+ nTreg cells (CD4+ YFP+) were sorted from 2-month-old female heterozygous wild-type and Foxp1/p4 cDKO spleens. RNA was isolated using RNeasy Plus Micro Kit (Qiagen) per manufacturers instruction. RNA-seq was carried out on Illumina HiSeq 2000 (Illumina), generating 100 base-pair single-end reads.