GSM8641431: Mpfer-1, replicate C; Marchantia polymorpha; RNA-Seq
Total RNA was extracted using TRI reagent (Ambion Life Technologies). Two μg of DNaseI-treated total RNA was used for generating the sequencing libraries. RNA libraries for RNA-seq were prepared at the Cologne Center for Genomics using the TruSeq stranded mRNA Kit (Illumina) according to manufacturer's instructions. ERCC spike-ins controls (ERCC RNA Spike-In Mix; Thermo Fischer) were used.