Novel NUDCD1 gene variant predisposes to severe COVID-19 disease in Asians
DNA concentration and purity were quantified after extraction. 1g of genomic DNA was fragmented to give a distribution of 200-500 base pairs. Library preparation was done using Kapa DNA HTP Library Prep Kit. Hybridization of adapter ligated DNA was performed at 47oC, for 72h, to biotin-labelled probe included in the Nimblegen SeqCap EZ Human Exome Kit. Libraries were sequenced using the Illumina Hiseq4000 sequencing system and paired-end 151bp reads were generated for analysis.