GSM8733606: AD_16; Homo sapiens; RNA-Seq
Total RNA was extracted from skin tape stip samples using Qiagen miRNeasy Mini kit. The libraries were generated using TruSeq Stranded mRNA Library Prep kit following manufactory protocol. Briefly, mRNA was first extracted from 5ng of total RNA using oligo-dT magnetic beads and fragmented at high temperature. A cDNA library was then prepared from the fragmented mRNA by reverse transcription, second strand synthesis and ligation of specific adapters.