GSM8699845: Fresh CMP, biol rep 3; Mus musculus; RNA-Seq
For RNAseq analysis, 10000 cells of exCMPs or fCMPs were sorted into 1.5 mL tubes and lysed in 600 ul Trizol LS reagent (Thermo Fisher Scientific). Subsequently, RNA purification was carried out by Tsukuba i-Laboratory, LLC. Libraries were generated using the SMARTer cDNA synthesis kit (Takara). Library preparation and next-generation sequencing were carried out by Tsukuba i-Laboratory, LLC. Libraries were generated using the SMARTer cDNA synthesis kit (Takara) and the high-output kit v2 (Illumina), followed by sequencing on a NextSeq 500 sequencer (Illumina) at 2x 36 paired end reads.