Nip-4h-1 (CRX805084; CRA012855)
Total RNA was prepared into a high-throughput library by MGIEasy RNA Library Preparation Kit (MGI1000006383) and enriched for mRNA with Oligo(dT) beads. The enriched mRNA was fragmented into short fragments for first-strand cDNA synthesis. The purified cDNA fragments were ligated with sequencing adapters and the cDNA library was sequenced using an DNBSEQ-T7 technology by Beijing Genomics institution (Wuhan, Hubei, China)