P070_PBMC_04
Droplet digital PCR of gDNA to determine the proviral load, by quantifying the number of tax copies per genome. Limiting dilution of gDNA was performed based on the PVL. Single provirus amplification (SPA) was perfomred using a nested touchdown PCR protocol with primers staggered in the LTRs to amplify near full-length genomes. Agarose gel purification of amplified provirus. Amplified provirus was prepared for sequencing on a MinION using the ligation sequencing kit with barcode expansion pack. Basecalling of reads, demultiplexing of samples, and adapter trimming was performed using Guppy or Dorado.