H3-K27me3 Cut & Tag of primary human fibroblasts
H3-K27me3 Cut & Tag of primary human fibroblasts
Trypsinized cells and used 50,000 for Cut & Tag with the nuclear permeabilization protocol described by the Henikoff lab.  Phenol-chloroform extraction after the Cut & Tag reaction followed by ethanol precipitation. PCR amplification of Cut & Tag fragmented DNA using Illmina multiplex oligonucleotide primers.