GSM9069146: Spt16 Myc ChIP-seq, ChIP DNA: spt6Δ2-238 pob3-E154K, IAA rep 3; Saccharomyces cerevisiae; ChIP-Seq
Cells were fixed with 1% formaldehyde for 30 minutes. Quenching, cell lysis, and chromatin extraction were performed as previously described (Gopalakrishnan et al 2019). Chromatin was sonicated using a Covaris E220e sonicator for 450 seconds per sample with a peak power of 140, a duty factor of 5, and 200 cycles per burst. Sonication was confirmed by agarose gel electrophoresis. Each chromatin sample was mixed with 10% S. pombe chromatin (strain FWP570) by protein mass for spike-in normalization. Libraries were constructed using the NEBNext Ultra II DNA library Prep Kit for Illumina (New England Biolabs) following manufacturer specifications.