GSM8949566: A3703_1344_AD2_NOC_Taxol_R2; Homo sapiens; RNA-Seq
Total RNA was isolated from frozen cell lysates and distributed across 18 96-well plates. Extracted total RNA was used to generate cDNA libraries following the manufacturer's instructions for the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen, Austria, upgraded protocol Feb 2017). Libraries were amplified (16 PCR cycles) and ERCC Spike-In Control Mixes (92 transcripts, ThermoFisher 4456740) were added.