GSM8733466: SLEC OT-I cell, Rroid2KO, Day 14, replicate2; Mus musculus; RNA-Seq
The OT-I cells were FACS-sorted directly into 700ul RTL lysis buffer supplemented with β-mercaptoethanol. RNA isolation was performed using the RNeasy Micro kit (Qiagen, #74004). RNA-seq libraries were prepared using the SMART-Seq Stranded Kit (#634442) and Unique Dual Index Kit 1–24 (#634756) according to the manufacturer's protocol (Takara Bio).