GSM9696630: human primary aortic smooth muscle cells, TCF7L2 knockdown, rep1; Homo sapiens; RNA-Seq
Total RNA was isolated from HASMCs using the RNeasy Mini Kit (QIAGEN, #74106). RNA quality was assessed using an Aigilent Bioanalyzer. 1.0 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared with the NEBNext Ultra RNA Library Prep Kit (NEB, #E7770).