Haemaphysalis bancrofti 6HB1 Australian adult female tick gut shotgun metagenomic sequencing
Shotgun metagenomic sequencing of whole Australian tick guts for microbiome analysis and assembly of endosymbiont MAGs. Tick guts were dissected, homogenised and passed through 0.45 or 0.8 m PES filters (Sartorius, Germany). Nymph samples were pooled where indicated, adult ticks were processed individually. DNA was extracted from the tick guts using Qiagen DNeasy Blood & Tissue (Qiagen, USA) following the manufacturers protocol optimized for arthropod tissues. Illumina DNA library preparation and DNA sequencing (NovaSeq 6000 SP 2x150 bp flowcell) were performed all the ticks.