GSM8521644: OT-1-caIL12_FBC; Mus musculus; RNA-Seq
For TIL isolation, the tumors were resected, placed into complete RPMI medium, and kept on ice. After weighing, the tumors were processed into single cells using a tumor cell isolation kit (130-110-187, Miltenyi Biotech, Auburn, CA, USA) following the manufacturer's instructions. A debris removal step was incorporated following the manufacturer's protocol (130-109-398, Miltenyi Biotech, Auburn, CA, USA). After this step, the Dead Cell Removal Kit (130-090-101, Miltenyi Biotech, Auburn, CA, USA) protocol was combined with a Pan T Cell Isolation Kit II (130-095-130, Miltenyi Biotech, Auburn, CA, USA), and an additional 1 µL of anti-Ter119-biotin (130-112-915, Miltenyi Biotech, Auburn, CA, USA) and anti-CD105-biotin (130-112-637, Miltenyi Biotech, Auburn, CA, USA )were added to the antibody cocktail. Libraries werePrepared according to the 10x Genomics Chromium Next GEM Single Cell 5'v2 (Dual Index) Kit User Guide