short read sequencing of marine plankton metagenome: sample 183, 1/4 S4 lane
DNA was extracted from Sterivex filters using a protocol that included lysozyme incubation, followed by Proteinase K and SDS treatment, and DNA precipitation with sodium acetate and isopropanol. The DNA was then purified using the Zymo Genomic DNA Clean and Concentrator kit and quantified with a Qubit fluorometer. Short-read metagenomic sequencing was performed with the Nextera DNA Flex Library Prep Kit, and the libraries were pooled, size-selected, and sequenced using an Illumina NovaSeq 6000. Raw reads underwent quality control using Trimmomatic and Bbduk, followed by metagenomic processing using JGIs pipeline, including error correction and assembly with metaSPAdes.