GSM8950903: CTRL dorsal forebrain progenitor cells, E16.5,  ATAC-seq, Sample 1; Mus musculus; ATAC-seq
The Zymo-Seq ATAC library kit was used for both extraction and library construction. Dorsal forebrain neural progenitor cells were isolated from embryonic mouse brains via magnetic-activated cell sorting (MACS) using Prominin-1 (Prom1) as the cell surface marker. Isolated cells were lysed to release nuclei, and transposition was performed to fragment chromatin while tagging accessible DNA regions with sequencing adapters. ATAC-seq libraries were prepared using the Zymo-Seq ATAC Library Kit (Zymo Research) following the manufacturer's instructions. Transposed DNA fragments were purified, PCR-amplified to incorporate indexed adapters, and size-selected to enrich for fragments representing accessible chromatin. Final libraries were assessed for quality and quantified before sequencing.