Transcriptomic dataset of BMP4, BMP9, BMP10, and BMP8b signaling in mature brown and white adipocytes
Transcriptomic dataset of BMP4, BMP9, BMP10, and BMP8b signaling in mature brown and white adipocytes
At the end of the treatment time, the cells were washed with phosphate-buffered saline (PBS) and snap-frozen on dry ice. Frozen cells were stored at -80°C until RNA extraction. The extraction was performed using the RNeasy Plus Mini Kit (Qiagen, 74104), according to the manufacturer's instructions. The cells underwent lysis in 350 μl of RLT buffer supplemented with 1% (v/v) β-mercaptoethanol (BME).  RNA libraries were prepared for sequencing by Novogene, processed to construct libraries suitable for the RNASeq analysis and following the standard protocols for RNA library construction. RNA integrity was confirmed by Bioanalyser analysis.