GSM9067213: leaves_ZT106_replicate2; Arabidopsis thaliana; RNA-Seq
For each sample 6-10 seedlings were snap frozen in liquid nitrogen and then cryodissected to isolate the shoot apex and true leaves.Total RNA was isolated from each sample using the RNeasy Plant Mini kit (Qiagen, Cat# 74904) and mRNAs were purified from 0.46µg of total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Cat# E7490) before library preparation. Library was prepared according to the Library Prep Kit (New England Biolabs, Cat# E7770). Sera-Mag Magnetic SpeedBeads (Thermo Scientific, Cat# 65152105050250) were used for all size selection and clean-up steps, and the resulting libraries were pooled and sequenced (single end 50 bp, SE50) on a HiSeq 2500 machine (Illumina).