LGM328_Bottom_13_Sep23 full-length 16S rRNA amplicon from bottom seawater collected 2023-09-13
Environmental DNA-based full-length 16S rRNA amplicon sequencing from 500 mL seawater collected at a fixed estuarine-influenced subtropical coastal station south of the Itajai-Acu estuary, southern Brazil. Water was collected with a 10-L Niskin bottle and sequentially filtered through 0.7 um glass fiber and 0.22 um cellulose acetate filters. DNA was extracted with the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit. The 16S rRNA gene was amplified with primers 27F and 1495R with 5-bp barcodes. Libraries were prepared with SQK-LSK114 and sequenced on Oxford Nanopore MinION using Flongle flow cells (FLO-FLG114). Basecalling used the super accurate model and reads were filtered at minimum Q8. Sample details: bottom water collected on 2023-09-13; season Winter; internal code LGM328; original sample_id F_13_Set23; eDNA concentration 27.7 ng/uL; DNA 260/280 ratio 1.9; sequenced reads 928.