Amplicon of PRRSV-2 infecting Prevacent PRRS vaccinated animal ID 31 of batch 2 on 6 dpi
Viral RNA was extracted from 200 L of serum using the NucleoMag Virus kit and KingFisher Flex. cDNA synthesis involved Template Switching RT Enzyme with PRRSV-2 lineage 1-specific primers, followed by second-strand synthesis using PrimeSTAR HS Polymerase. cDNA was purified using CleanNGS beads and quantified via Qubit. Sequencing libraries were prepared using the Rapid Barcoding Sequencing Kit and sequenced on Oxford Nanopore's GridION platform with R.10 flow cells for 24 hours, with separate libraries for each experimental group (non-immunized positive control, and two vaccinated groups).