P-DBM (CRX1931982; CRA029312)
Single-cell suspensions were prepared from the midgut of P-DBM. The suspensions were processed using the Chromium Single Cell 3' Kit v3 on a 10x Genomics controller to generate GEMs. Following reverse transcription in GEMs, cDNA was purified, amplified, and then fragmented. Libraries were constructed through end repair, adapter ligation, and sample index PCR. Final libraries were purified, quality-checked on an Agilent Bioanalyzer, and quantified by Qubit before being pooled for sequencing on an Illumina NovaSeq 6000 in PE150 mode.