RNA-Seq of Aedes scapularis
Nucleic acids were extracted using the ZR & ZR-96 Viral DNA/RNA Kit (Zymo Research, CA, USA) according to the manufacturers instructions, followed by cDNA synthesis using the AMV Reverse Transcription Kit (Promega, WI, USA). The second strand of cDNA was synthesized using the DNA Polymerase I Large Fragment Kit (Promega, WI, USA). Deep sequencing for library construction was performed with the HiSeq 2500 Sequencer (Illumina, CA, USA) with 126 base pair reads, and bioinformatics analysis was conducted according to the protocol described by Deng et al. 2015. Contigs were then analyzed via BLAST (BLASTn and BLASTx) for comparison with viral sequences available in GenBanks Virus.