GSM8521860: mCherry_Pum2-ELP-GFP_condensateRNA_sample1; Escherichia coli; RNA-Seq
35mL of cells sonicated 2min 10 on 40 off 85% amplitude. Condensates extracted with OptiPrep density gradient ultracentrifugation at 100k x g overnight at 37C. RNA extracted with TRIzol LS reagent. KAPA RNA Hyperprep kit with manufacturer's instructions (Roche). 6min fragmentation step, 10 library amplification cycles.