GSM9084903: Y1_Naive_scATAC; Mus musculus; ATAC-seq
EpdSCs (CD49f+, Sca-1+, CD29+, CD34-, CD45-, CD117-, CD140a-, CD31-) were FACS isolated and nuclei extracted using 10x protocol for nuclei extraction. Following nuclei extraction downstream processing was performed by the Integrated Genomics Operation (IGO) at Memorial Sloan Kettering Cancer Center where single cell ATAC-seq was performed. Single-cell ATAC-seq libraries were prepared using the 10x Genomics Chromium Single Cell ATAC Solution (v1.1) according to the manufacturer's protocol. Briefly, nuclei were isolated from fresh/frozen tissue, tagmented with a Tn5 transposase loaded with sequencing adapters, and then encapsulated into Gel Bead-In Emulsions (GEMs) for barcoding. Following barcoding, libraries were PCR-amplified and purified. Final libraries were quantified and sequenced on an Illumina platform using paired-end sequencing.