GSM9696055: tg(Grm1)Epv, DC boost, biological replicate 2; Mus musculus; RNA-Seq
For RNA-sequencing (RNA-seq) analysis, EPv tumor/ear samples were weighed and processed using the RNeasy Mini Kit (Qiagen, Hilden, Germany). Frozen samples were disrupted and homogenized on ice in an appropriate volume of lysis buffer according to the manufacturers' recommendations by using a Tissue Ruptor (Omni TH Tissue Homogenisierer). Lexogen QuantSeq 3'-mRNA libraries were prepared at the MultiOmics Sequencing Core Facility (Medical University of Innsbruck) following the manufacturer's instructions (Lexogen GmbH, Vienna, Austria), quality validated, multiplexed and sequenced with a Illumina NovaSeq sequencer (Azenta, Leipzig, Germany) at 150 bp read length.