GSM8730650: APOE3_DGAT_1_2_inh_LPS_REP3; Homo sapiens; RNA-Seq
Cells were seeded at a density of 5 x105 cells per well in a tissue culture-treated 12-well plate (NEST). Microglia were treated with LPS, DGAT inhibitors, or DMSO vehicle as described above. Cells were harvested after treatments in 300 μL of TRIzol Reagent (Invitrogen) and RNA was purified using Direct-zol RNA isolation kits (Zymo Research). The Illumina RNA Stranded PolyA library kit was used followed by sequening using the NextSeq 2000.