GSM9035379: NH4, 15min, rep1; Nostoc sp. PCC 7120 = FACHB-418; RNA-Seq
Total RNA was isolated using hot phenol, phenol-chloroform and chloroform extractions. 23 fmol of a PhiX spike-in RNA mix was added to 1 μg of total RNA that was later treated with Turbo DNAse (Ambion) to eliminate DNA traces. RNA libraries for RNA-seq were prepared using Illumina Stranded TOTAL RNA preparation RIBO-ZERO PLUS kit following manufacturer's protocols.