FR113N ETOH R3 male body 3' mRNA-Seq
RNA was extracted from the bodies of 30 male flies using the NucleoZol one phase RNA purification kit (Macherey-Nagel). Library preparation and sequencing was performed by Lexogen (Vienna, Austria) using the QuantSeq 3 mRNA-Seq Library Prep Kit REV. This kit generates Illumina-compatible libraries capturing sequences at the 3 end of polyadenylated RNA. The libraries were barcoded and pooled, and approximately two million reads were generated per library on the Illumina NextSeq. The data was demultiplexed prior to delivery.