Long Read ONT WGS of Enterococcus faecalis bacteriophage insensitive mutants (BIM) from A New Arsenal Against E. faecalis: identification of novel Phages Combatting Multidrug Resistant clinical isolates
The extracted BIMs DNA was sequenced with long-reads using the Oxford Nanopore Technologies (ONT) platform on a MinION Mk1B (Oxford Nanopore Technologies, Oxford, UK) device. Specifically, the Rapid Barcoding Kit (Oxford Nanopore Technology, SQK-RBK 110.96) was used to sequence E. faecalis whole genomes on R9.4.1 MinION flow cells (Oxford Nanopore Technology), using 100 ng of the isolated DNA. Base-calling was conducted with Dorado v0.5.0, using the dna_r9.4.1_e8_sup@v3.3 base caller model (Oxford Nanopore Technology).