GSM8640444: Spc Cells, H3K27ac, Control, rep2; Mus musculus; OTHER
Chromatin profiling was performed using the CUT&Tag protocol as described by Kaya-Okur et al. (2020). Briefly, cells were immobilized on concanavalin A-coated beads and incubated with a primary antibody specific to the histone modification of interest. Following binding with a secondary antibody, protein A-Tn5 transposase was added 1:400 to introduce sequencing adapters at target sites. DNA was extracted using Zymo DNA Clean & Concentrator (Cat. No.: D4014) and ibraries were amplified by PCR using 15 amplification cycles.