GSM9067535: Th1 cells, CBS+66, CTCF-binding site+66 deletion; Mus musculus; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was treated with Mnase. ChIP-seq libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit  (NEB) and NEBNext Multiplex Oligos for Illumina (NEB). Briefly, 70 ng ChIP DNA fragments were end-repaired, indexed, and amplified. Libraries were sequenced with 150-bp paired-end reads on a NovaSeq platform.