Pseudomonas aeruginosa (AH18I053)
Isolates were first cultured on cation-adjusted Mueller-Hinton II agar, then sub-cultured in cation-adjusted Mueller-Hinton broth (Becton-Dickinson). Genomic DNA was extracted using the GenFind V3 Reagent Kit (Beckman Coulter). Short-read sequencing libraries were prepared using the Nextera Flex DNA Library Prep Kit (Illumina), followed by 150 bp paired-end sequencing on the NovaSeq 6000 system (Illumina).