RNA-Seq of Pampus argenteus: adult caudal fins
mRNA was enriched from total RNA using Oligo dT magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers. Then, second-strand cDNA was synthesized using dUTP instead of dTTP. After end repair, A-tail addition, joint joining, fragment selection, amplification, and purification, the chain-specific library is ready.