GSM9218680: CD4+T cells under Th1 condition for 72h with ALX-5407 treatment_biol rep 1; Mus musculus; RNA-Seq
Total RNA were extracted using Trizol, RNA integrity was verified using the Agilent Bioanalyzer 2100. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection.