GSM9069593: RNA-seq in SP6KO bud ectoderm E10.5, rep2; Mus musculus; RNA-Seq
Ectoderms were isolated with fine forceps from E10.5 forelimb buds of Sp8KO, SP6KO and WT embryos, after digestion with 0.25% trypsin for 20 min. on ice. The ectoderms of ~2 forelimb buds were used for each replicate. SMART-Seq v4 Ultra Low Input RNA for Sequencing, Nextera XT DNA Library Preparation Kit