GSM8768522: KO52, EV, DMSO, ATAC-seq, R2; Homo sapiens; ATAC-seq
Nuclei from 50,000 cells were extracted by lysis in 10mM Tris pH 7.4, 10mM NaCl, 3mM MgCl2, 0.1% NP-40, 0.1% Tween-20 and 0.01% digitonin. After a wash, samples were transposed using an in-house synthetized Tn5 and loaded with stantard illumina compatible adaptors, 30 min, 37 °C. DNA was purified by Zymo ChIP DNA Clean and Concentrator Kit. Transposed fragments were amplified with NEBNext High-Fidelity PCR Master Mix (NEB M0541). Libraries were cleaned and size-selected using CleanNGS beads (CNGS-0050) and assessed by Tapestation and Qubit.