GSM8835708: 41B_VH_6NJ_HomeCage; Mus musculus; RNA-Seq
RNA was extracted using Trizol (Qiagen, Valencia, CA, USA) and RNAeasy extraction kit (Qiagen, Valencia, CA, USA), as previously described(Yazdani, Shen et al. 2016, Goldberg, Zeid et al. 2021). RNA concentration and quality were evaluated using NanoDrop2000 (NanoDrop, Wilmington, DE, USA) and Agilent Bioanalyzer (Agilent, Santa Clara, CA, USA) Libraries were prepared by the Huck Institutes of the Life Sciences Genomics Core Facility (Penn State University) for 75 bp single-end reads using the Illumina TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA).