GSM9181797: Artificially reared hypothalamus RNA, PND13, replicate 3; Mus musculus; RNA-Seq
Total RNA was extracted using the NucleoSpin RNA kit (Takara Bio, Shiga, Japan) from four MR and three AR pups, each derived from three different litters. RNA libraries for RNA-seq were prepared using the SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (Takara Bio) and the Nextera XT DNA Library Prep Kit (Illumina) with IDT for Illumina DNA/RNA UD Indexes, following the manufacturers' protocols.