GSM9393574: bulk RNA-seq LATS KO 4; Mus musculus; RNA-Seq
Liver tissues from NASH-treated three WT and three Cyr61DHep mice were measured by bulk transcriptome sequencing. RNA was isolated using Total RNeasy Mini Kit (Qiagen). RNA-seq libraries were prepared using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina. RNA-seq libraries were validated by MedGenome (San Francisco, CA) prior to being sequenced on a Nova-Seq 6000 (Illumina).