Bovine (bos) RNA-seq: Mammary glands of adult females
Briefly, RNA concentration and purity were assessed using a NanoDrop ND-1000 spectrophotometer, and RNA integrity was evaluated before library construction.   After removal of ribosomal RNA, the remaining RNA was fragmented and used for first-strand and second-strand cDNA synthesis.   The resulting double-stranded cDNA was end-repaired, A-tailed, ligated to sequencing adapters, and amplified by PCR to generate sequencing libraries with an average insert size of approximately 300  50 bp.