Single-cell and single-nucleus RNAseq of the human and mouse skeletal muscle across age
Single-cell and single-nucleus RNAseq of the human and mouse skeletal muscle across age
Three 19 months old and five 3 months old male mice of C57BL/6JRj strain were obtained from Janvier labs, France.They were used to dissect hindlimb muscles for the single-nucleus isolation according to the following protocol: https://www.protocols.io/view/nuclei-isolation-from-human-skeletal-muscle-kqdg34pqel25/v1. Briefly, muscle tissue was grounded using a dounce homogenizer, lysed in the nuclei lysis buffer and, finally, Percoll gradient was used to separate intact nuclei for loading and cell debris. Cell or nuclei suspension were  loaded on the Chromium Controller (10X Genomics) with a targeted cell or nuclei recovery of 8000.  10X Genomics Single Cell 3â€™ v2 or v3 Reagent Kit was used to create GEM, perform cDNA synthesis and generate sequencing libraries according to standard instructions.