Langdon genome sequencing and assembly
Genomic DNA (gDNA) was extracted from seedling leaf tissues using the cetyltrimethylammonium bromide method. High-quality gDNA (10 g, 100 ng/l) was prepared for the next step of library construction. PacBio single-molecule real-time (SMRT) bell library preparation was performed using the SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences, CA, USA; PN 101-853-100) in accordance with the manufacturers instructions. The library was prepared for sequencing with a 30h movie on the Sequel IIe system (Pacific Biosciences) by the Berry Genomics Corporation (Beijing, China).