PacBio HiFi (CCS) whole genome sequencing of Ilex integra female plant
High molecular weight genomic DNA (>30kb) was extracted from young leaves. DNA was sheared to 15-18kb fragments, purified, end-repaired and ligated with SMRTbell adapters. Sequencing was performed on PacBio Sequel II platform using Single-Molecule Real-Time (SMRT) technology with Zero-Mode Waveguides (ZMWs). Circular Consensus Sequencing (CCS) mode was used to generate high-quality consensus reads with accuracy >99% (Q20). This is raw PacBio CCS BAM file without genome alignment.