hic of Du an catfish
Fresh muscle tissue was used for Hi-C library construction. Briefly, chromatin was cross-linked with paraformaldehyde and digested with DpnII. The resulting sticky ends were filled in with biotinylated nucleotides and subjected to proximity ligation to generate chimeric DNA fragments. After reversal of cross-links and DNA purification, biotin was removed from non-ligated DNA ends, and the biotinylated ligation products were captured with streptavidin beads and amplified by PCR. The resulting Hi-C library, with an insert size of 400 bp, was sequenced on the Illumina NovaSeq 6000 platform using a paired-end 150 bp strategy.