GSM9871086: RNA-seq, MDA-MB-231, SRPK2 overexpression (OE), biol rep 1; Homo sapiens; RNA-Seq
The selected treatment cells were subjected to total RNA extraction using TRIZOL (Ambion). The RNA was further purified with two phenol-chloroform treatments and then treated with RQ1 DNase (Promega, Madison, WI, USA) to remove DNA. The quality and quantity of the purified RNA were determined by measuring the absorbance at 260 nm/280 nm (A260/A280) using Smartspec Plus (BioRad, USA). The integrity of RNA was further verified by 1.5% agarose gel electrophoresis. For high-throughput sequencing, the libraries were prepared following the manufacturer's instructions and sequenced on an Illumina NovaSeq 6000 system in paired-end mode.