GSM9326132: VC KPS14 WT RNAseq in LB medium replicate 3; Vibrio cholerae; RNA-Seq
Total RNA was isolated with Tri-reagent (Sigma) according to the manufacturer and DNase digested (ThermoFisher scientific, #AM2238). rRNA was depleted using rRNA-specific biotinylated probes according to Culviner et al 2020 (DOI: 10.1128/mbio.00010-20)/RNA libraries for RNAseq were prepared using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (New England Biolabs, #E7300)  following manufacturer´s protocol