GSM9476080: CD4 T cell donor 2 replicate 1 rna; Homo sapiens; RNA-Seq
RNA was extracted using the Zymo Direct-Zol RNA MiniPrep kit, followed by rRNA depletion with the RiboMinus Eukaryote Kit v2. The RNA was then partitioned into plus and minus libraries using the NEBNext Poly(A) mRNA magnetic isolation module. Poly(A) minus libraries were prepped for NGS sequenicng using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina.