RNA-Seq of Cranberry Hybrids, Heat Treatment: 309_19, 15 minutes, replicate 1
Total RNA was isolated from leaves of Vaccinium macrocarpon, V. oxycoccos, and F1 hybrids subjected to heat and cold stress, using a CTAB-based extraction followed by LiCl precipitation and DNase treatment. Libraries were prepared from 200 ng of RNA per sample using the Oxford Nanopore Technologies (ONT) cDNA-PCR Sequencing Kit, with 16 cycles of barcoded PCR amplification. Pooled cDNA was adapter-ligated and sequenced on ONT PromethION R10.4.1 flow cells using V14 chemistry to generate full-length cDNA reads. This study aims to characterize transcriptional stress responses and hybrid gene expression in cranberry.