GSM9474732: LN18-2; Homo sapiens; RNA-Seq
RNA was extracted using TRIzol reagent (Thermo Fisher Scientific). Approximately 1.0 µg of total RNA per sample was used for sequencing libraries. Poly(A) mRNA was isolated using oligo(dT) beads and fragmented at high temperature with divalent cations. First- and second-strand cDNA were synthesized, end-repaired, A-tailed, and ligated to adapters, followed by PCR amplification and size selection. Strand-specific information was retained (reverse).