LGM329_Surface_30_Oct23 full-length 16S rRNA amplicon from surface seawater collected 2023-10-30
Environmental DNA-based full-length 16S rRNA amplicon sequencing from 500 mL seawater collected at a fixed estuarine-influenced subtropical coastal station south of the Itajai-Acu estuary, southern Brazil. Water was collected with a 10-L Niskin bottle and sequentially filtered through 0.7 um glass fiber and 0.22 um cellulose acetate filters. DNA was extracted with the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit. The 16S rRNA gene was amplified with primers 27F and 1495R with 5-bp barcodes. Libraries were prepared with SQK-LSK114 and sequenced on Oxford Nanopore MinION using Flongle flow cells (FLO-FLG114). Basecalling used the super accurate model and reads were filtered at minimum Q8. Sample details: surface water collected on 2023-10-30; season Spring; internal code LGM329; original sample_id S_30_Out23; eDNA concentration 13.4 ng/uL; DNA 260/280 ratio 2.1; sequenced reads 505.