GSM9084582: Resource Total RNA TM71, Rep2; Homo sapiens; RNA-Seq
We performed total RNA-sequencing on three samples each for the resource, dexamethasone control, and dexamethasone treatment experiments. For each experiment, primary TM cells were trypsinized, flash frozen and kept at -80°C. Total RNA was isolated using Qiagen RNeasy Mini Kit (Germantown, MD, USA; cat# 74104) and the quality of RNA was determined using Agilent TapeStation (Santa Clara, CA, USA). Libraries were prepared from 10ng of total RNA using the Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus kit (San Diego, CA, USA; cat# 20040525). Libraries were sequenced on an Illumina NextSeq 2000 platform.