GSM9837142: Macrophages_DNA_SCIENCE; Homo sapiens; OTHER
Nuclei were distributed into 96-well plates by limiting dilution. Nuclear membranes were lysed and chromatin was reverse-crosslinked by incubation with Proteinase K for 4 h at 65 °C followed by 15 min 95 °C to deactivate Proteinase K. DNA interactomic library preparation was performed using a two-step PCR approach. Locus-specific amplification was conducted using custom primers (Supplemental Table 1, PCR Round 1) containing Nextera-compatible universal adapter handles. Multiplexing was achieved in a second PCR step using Illumina DNA/RNA Unique Dual Indexes (UDI) Set A barcodes (Supplemental Table 1, PCR Round 2).