GSM8640613: LCMVc13 infection,GFI1-KO, D3, rep3; Mus musculus; ATAC-seq
Nuclei were washed and resuspended in 50 μL 1× TDE1 buffer (Illumina) containing 2.5 μL TDE1 transposase (Illumina). The transposase reaction was conducted at 37 °C for 30 min, with mild shaking. Library amplification and barcoding were performed with NEBNext Ultra II Q5 Master Mix (New England Biolabs) using IDT dual index primer set (Integrated DNA Technologies). PCR was conducted for 10–11 cycles. Library purification was performed with the MinElute PCR Purification Kit (Qiagen) and library size distribution was assessed using the TapeStation High Sensitivity DNA Kit (Agilent).