GSM9474764: MiaPaCa2_CCRX_ctrl_1; Homo sapiens; RNA-Seq
The cells were lysed by adding 100 μL of RL Lysis Buffer from the innuPREP RNA Mini Kit 2.0 to each well. Cell lysates were processed by the manufacturer's instructions from the kit mentioned above. RNA concentrations were measured using the DS-11+ Spectrophotometer (DeNovix). The samples were then stored at -80°C until further use. RNA-seq library is constructed with a TruSeq RNA library preparation kit following relevant protocol.