GSM9657562: tumor-infiltrating type 1 conventional dendritic cells, Dyrk1a knock out, Day13, rep2; Mus musculus; RNA-Seq
Total RNA were extracted using Life-Trizol. A total amount of 500 ng RNA per sample was used as input material for the RNA sample preparations. Messenger RNA (mRNA) was enriched from total RNA using oligo(dT) magnetic beads. Following fragmentation, first-strand cDNA was synthesized using random hexamer primers, followed by second-strand cDNA synthesis. Subsequently, the cDNA fragments underwent end repair, A-tailing, adapter ligation, size selection, PCR amplification, and purification to generate the final sequencing library.