Ao-3 (CRX703152; CRA011015)
Total genome DNA from samples was extracted using CTAB method. DNA concentration  and purity was monitored on 1% agarose gels. According to the concentration, DNA was diluted  to 1ng/microL using sterile wate.16SrRNA genes of distinct regions(16S V3/V4) were amplified used specific primerwith the barcode. All PCR reactions were carried out with 15 microL of Phusion High-Fidelity PCR Master Mix (New England Biolabs); 2 microM of forward and reverse primers, and about 10 ng template DNA.