B. japonicum ACCC 15027
Take 100ng of whole gene gDNA, ultrasonic crusher (Covaris S220) randomly interrupted into fragments smaller than 500bp, then end repair by End Prep Enzyme Mix (including 5' end phosphorylation and 3' end plus 'A '), with sequencing junctions at both ends, followed by purification of the target fragment using magnetic beads, and finally amplification with P5 and P7 primers. The quality of the libraries was checked using an Agilent 2100 Bioanalyzer (Agilent Technologies, PaloAlto, CA, USA), and the concentration of the libraries was checked by Qubit 3.0, and cluster preparation and sequencing were performed with the qualified libraries.