control_R15
The sequencing library was prepared following the guidelines for NEBNext mRNA magnetic isolation module purification kit (E7490) and NEBNext Ultra II directional RNA library prep kit for Illumina (E7760). The quality of RNA was estimated using Bioanalyzer 2100 prior to sequencing. The NEBNext Multiplex Oligos primer set for Illumina (Index Primers Set 2) (E7500S) was used for preparation. At all stages, the material was cleaned using AMPure XP beads.