GSM8768481: FRDA heart treated by replacement with healthy macrophages_1 (FA treated 1); Mus musculus; RNA-Seq
Total RNA was extracted from one minced mouse heart tissue for bulk RNA-seq Whole heart tissue was collected from WT and YG8s800 mice at 20- and 22-weeks post-bone marrow transplantation (BMT), following conditioning with Busulfan (BU) and PLX3397. The time points corresponded to an age of 7–8 months for each group. Following two washes with cold 1x PBS, the heart tissues were dissected in cold 1x PBS using a sterile knife to facilitate efficient RNA extraction. Total RNA was extracted from the minced heart tissue of each mouse group using the Invitrogen™ PureLink™ RNA Mini Kit (#12-183-018A), following the manufacturer's instructions for RNA purification. Library preparation and sequencing was conducted by MedGenome Inc. (Foster City, CA, USA) using the Illumina Stranded mRNA Prep kit and Novaseq 6000 sequencer (Illumina, San Diego, CA) with paired-end 100 base pair (bp) reads.