GSM8698703: Muscle, Adult Dermatomyositis; Homo sapiens; OTHER
RNA was extracted from tissue sections using the RNeasy Plus Micro Kit (Qiagen). The tissue was scraped from slides and homogenized in lysis buffer, followed by RNA extraction according to the manufacturer's protocol. RNA quality was assessed using a Bioanalyzer (RIN>=7) and RNA concentration was measured using a Qubit RNA assay. If RNA input was low, RNA was amplified using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech). Tissue sections (10 μm thick) were prepared on poly-L-lysine-coated slides and stored at -80°C. After thawing, tissues were permeabilized to allow efficient RNA capture. The mRNA was hybridized with spatially barcoded probes, followed by reverse transcription to generate cDNA. The cDNA was then amplified, fragmented, and ligated with adapters for sequencing. The library quality was assessed using a Bioanalyzer, and sequencing was performed on the Illumina NovaSeq 6000 platform with paired-end reads.