GSM8641162: Exosomal small RNA, HFD biological replicate 2; Mus musculus; miRNA-Seq
Exosomal RNA was purified using phenol extraction followed by ethanol precipitation. The RNA quality and integrity were evaluated using the Agilent 2100 Bioanalyzer. The resulting RNA was sequenced by Novogene, which performs additional quality control steps to ensure data accuracy, including RNA integrity checks and quantification. RNA within the 25–200 nucleotide range was selected for library preparation. Libraries were prepared using the Abclonal Small RNA Library Prep Kit for Illumina.