Amplicon SMF for Pol II occupancy project
Amplicon SMF for Pol II occupancy project
Mouse ES cells were cultured on 0.2% gelatin-coated plates in ES medium (DMEM, supplemented with 15% FBS, LIF, 2-Mercaptoethanol, 2 mM L-Glutamine and 1x non-essential amino acids) at 37C and 5% CO2. Medium was changed daily and cells were split every second day. Dual-enzyme SMF was performed following the protocol from Kleinendorst et al., 2021; Soenmezer et al., 2021.