Didymella segeticola treated with PDB and sterile water for 12 h
Mycelial plugs (4 mm diameter) from the edge of 7-day-old colony of the strain CGMCC3.20152 was transferred to conical flask with PDB, and shaked at 180 r/min, 25C for 60 h in darkness. Mycelia treated with PDB for 12 h were collected and washed twice with sterile water, then were ground into fine powder under the protection of liquid nitrogen. One hundred mg mycelia powder were used for RNA extraction. Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. We performed the sequencing on Illumina Hiseq 4000 (LC Sceiences, USA) to yield 2 x 150 bp paired-end raw reads following the vendor's recommended protocol.