lcWGS of Paralithodes camtschaticus
Samples collected from 5 regional populations of Red King Crab in Alaskan waters of the North Pacific. DNA was extracted from muscle, heart, gill, and hepatopancrea. Briefly, input DNA was normalized to 10 ng for each individual, and libraries were purified and normalized using SequalPrep plates (ThermoFisher Scientific, Waltham, MA, USA). Normalized pooled libraries were subject to a 0.6x size selection, purification, and volume concentration with AMPure XP from Beckman Coulter. Samples were sent to the University of Oregon Genomics and Cell Characterization Core Facility for whole genome sequencing using paired-end 150-bp reads on an Illumina NovaSeq 6000 with S4 chemistry. Forty-eight individuals were multiplexed per lane to target a genome-wide depth of coverage of 3x per individual given the estimated ~6 GB genome size of red king crab based on the blue king crab genome (Tang et al., 2021).