Didymella segeticola treated with PDB and Ningnanmycin at EC50 concentration for 12 h
Mycelial plugs (4 mm diameter) from the edge of 7-day-old colony of the strain CGMCC3.20152 was transferred to conical flask with PDB, and shaked at 180 r/min, 25C for 60 h in darkness. Mycelia treated with PDB containing Ningnanmycin (EC50, final concentration: 1347.22 U/mL) for 12 h were collected and washed twice with sterile water, then were ground into fine powder under the protection of liquid nitrogen. Total RNA was extracted using Trizol reagent (thermofisher, 15596018) following the manufacturer's procedure. We performed the 2150bp paired-end sequencing (PE150) on an Illumina Novaseq 6000 (LC-Bio Technology CO., Ltd., Hangzhou, China) following the vendor's recommended protocol.