GSM9036139: CK(B73),leaf,4°C,24h 2; Zea mays; RNA-Seq
RNA integrity was assessed using the Bioanalyzer 2100 system(Agilent Technologies, CA, USA) was extracted from the leaves. Messenger RNA was purified from total RNA using poly-T oligo-attachedmagnetic beads.  After fragmentation, the first strand cDNA was synthesized using random hexamer primers.  Then the second strandcDNA was synthesized using dUTP, instead of dTTP.  The directional library was ready after end repair, A-tailing, adapter ligation, sizeselection, USER enzyme digestion, amplification, and purification.  Thelibrary was checked with Qubit and real-time PCR for quantificationandbioanalyzer for size distribution detection.