pectoral fin DUT treatment mRNA-seq replicate 2
Zebrafish pectoral fins were divided into pf1pf10 groups, with additional DUT and DMSO treatment groups, each with three biological replicates. Tissues were rinsed with PBS and preserved in RNAlater. Total RNA was extracted using standard protocols, and mRNA was enriched with oligo(dT) beads for transcriptome library construction. After quality control, libraries were sequenced on the Illumina NovaSeq 6000 platform with paired-end 150 bp reads.