16S sequencing of eelgrass microbiome: cotton swab
Microbial DNA was extracted from all field and experiment swabs using the ZymoBIOMICS DNA Microprep kit following the manufacturers recommended protocol. DNA extracts were sent on dry ice to the Microbial Interactions & Microbiome Center at the University of Washington for PCR amplification with barcoded primers targeting the V4-V5 region (515f = GTGYCAGCMGCCGCGGTAA and 926r = CCGYCAATTYMTTTRAGTTT) and sequencing on the Illumina NextSeq2000 with the P1 XLEAP-SBS reagent kit.