Small RNA Seq of homo sapiens: pediatric familial hypercholesterolemia
RNA was isolated from serum using the miRNeasy Serum/Plasma Kit with QIAseq miRNA Library QC Spike-ins for RNA isolation quality control. Sequencing libraries were prepared using the QIAseq smallRNA Library Prep Kit with Unique Molecular Identifiers (UMIs) and RNA Unique Dual Indices (RUDIs), QC-checked on an Agilent Bioanalyzer 2100 and quantified by Qubit dsDNA HS Assay. Pooled libraries were sequenced on an Ilumina NextSeq2000 instrument (1x76bp single-end) and reads were demultiplexed without adapter trimming or short read masking before alignment to miRBase_v21 and Homo sapiens (GRCh38) using the QIAgen GeneGlobe RNA-seq Analysis Portal 2.0.