GSM8836052: Exp2_LP_sgCont_QuantSeq_rep1; Mus musculus; RNA-Seq
Total RNA was isolated from samples of 3 x 105 cultured cells using a RNeasy Micro Kit (Qiagen). Five hundred nanograms of total RNA was subjected to the 3'mRNA library preparation with QuantSeq 3' mRNA-seq Library Prep Kit FWD (LEXOGEN) according to the manufacturer's instructions. After the PCR step, size distribution and yield of the library was determined by the D1000 high sensitivity tape station (Agilent) or Agilent High Sensitivity DNA kit on the bioanalyzer (Agilent). The pooled libraries were loaded on the Illumina Nextseq500 platform and analyzed by 75bp single read.