leaf sample
Hi-C data sequencing: the sample was subjected to formaldehyde-induced cross-linking, followed by digestion with the restriction enzyme DpnII (New England Biolabs, MA, USA). This step generated cohesive ends adjacent to the cross-linking sites. Subsequent steps included non-homologous end joining, circularization, DNA purification, capture, and library quality assessment. The final sequencing was performed on an Illumina NovoSeq 6000 platform (Benagen Technologies, Hubei, China), generating paired end reads of 150 base pairs each.