RNA Sequencing of Grains from Maize Inbred Line 08LF
RNA was extracted from three replicate samples of developing kernels at 6, 10, 14, 18, 21, and 24 DAS for NONPOL plants and at 10, 14, 18, 21, and 24 DAS for POL plants using the RNAprep Pure Plant Kit (TIANGEN). RNA-seq libraries were constructed using the Illumina TruSeq RNA Library Preparation Kit (Illumina, San Diego, USA). Single-end sequencing was performed on the Illumina HiSeq 2000 platform. The raw read data are available at the National Center for Biotechnology Information Sequence Read Archive (https://www.ncbi.nlm.nih.gov/sra; accession numbers PRJNA347500 and SRP091292). WGCNA (Langfelder and Horvath, 2008) was used to identify modules of highly correlated genes on the basis of the RNA-seq expression data. Cytoscape version 3.0.2 was used for network visualization and enrichment with various data, such as differential gene expression levels.