miR-184D Midgut
Sugar-fed females were dissected into Trizol. After homogenization with 2.8mm ceramic beads (CK28R, Precellys), RNA was extracted with the Direct-zol RNA Mini-prep kit (Zymo Research) including on-column DNase treatment. Four biological replicates per condition were subjected to RNA-seq. Libraries were prepared with the NEB Next Ultra RNA Library Prep Kit and sequenced on a NovaSeq 6000 Illumina platform (instrument HWI-ST1276) generating 150bp paired-end reads.