scRNA-seq of Dp16 lung (CRX781614; CRA012492)
The lung tissue was disassociated into single cells with Singleron PythoN Tissue Dissociation System. The obtained single cell suspensions were loaded onto microwell chip of the Singleron Matrix Single Cell Processing System to label mRNAs from individual cells. Barcoded mRNAs were then reverse transcribed to cDNAs and subjected to scRNA-seq libraries construction according to the protocol of the GEXSCOPE Single Cell RNA Library Kits (Singleron).