Mock_3h_R3
Fifteen-day-old wheat plants from the cultivar Titlis were treated with mock control (0.1% v/v UEP-100 (Croda) and 0.01% v/v Tween 20) for 3 hours. Total RNA was extracted using the TRIzol reagent (Invitrogen, Cat. No. 15596018), treated with DNase I (Qiagen, Cat. No. 792256) and purified using the RNeasy Plant Mini Kit (Qiagen, Cat. No. 74904). RNA quality check, library preparation (non-stranded polyA enrichment), and subsequent sequencing were performed by Novogene (Beijing, China)