GSM8947266: Tumor_EOC_31; Homo sapiens; RNA-Seq
Total RNA was extracted from fresh-frozen tumor tissue using the RNeasy Lipid Tissue Mini Kit (Qiagen). Quality control of the extracted RNA was conducted using the 4200 Tapestation system (Agilent) and the Qubit RNA HS Assay Kit (ThermoFisher Scientific) Sequencing libraries were prepared from 100 ng total RNA using the Illumina Stranded Total RNA library preparation kit with Ribo-Zero Plus treatment (cat# 20040525/20040529, Illumina Inc.). Unique dual indexes (cat# 20040553/20040554, Illumina Inc.) were used. The library preparation was performed according to the manufacturers' protocol (# 1000000124514).