Genome-wide CRISPR/Cas9 screen in A549 cells to identify important host factor for IAV infection
PCR amplification products without sequencing adapters were subjected to quality assessment, and 200-500 ng DNA fragments were used for library preparation. The fragments underwent end repair, 5 phosphorylation, dA-tailing, adapter ligation, clean-up, and PCR enrichment. The final libraries were sequenced on the Illumina platform using high-throughput sequencing.