GSM9712942: Ascending aorta and aortic arch, Female; Mus musculus; RNA-Seq
Aortic tissues were harvested from six female or male Wnt1-Cre/Rosa26-mTmG mice and pooled by sex. Each pooled sample was divided into two technical replicates. Tissue homogenization was performed in ice-cold Nuclei EZ Lysis Buffer (Sigma-Aldrich, U.S.A.), followed by incubation on ice, centrifugation, and subsequent washes in nuclei suspension buffer (NSB). The nuclear suspensions were filtered and adjusted to a final concentration of 1,000 nuclei/μl. All nuclear isolations and preparations were completed within 30 minutes, and the samples were loaded onto BMKMANU C1000 chips. SnRNA-seq libraries were generated using the BMKMANU DG1000 GEM Kit (Biomarker, China) on a Droplet Generation Instrument, following the manufacturer's protocol. Library quality was assessed using Qubit 4.0 (Invitrogen, U.S.A.) and Agilent 2100 Bioanalyzer (Agilent Technologies, U.S.A.).