GSM8698456: PBMC 28 days after TCR-T infusion; Homo sapiens; RNA-Seq
PBMCs or BMMCs were thawed, washed in PBS and resuspended in 0.04% BSA by volume. Cells were loaded on a 10x Chromium Controller based on the 3' Chromium or 5' Chromium Single Cell V(D)J Reagent Kit manual (10x Genomics). Library preparation was performed as per manufacturer's protocol with no modifications. Library quality was confirmed by TapeStation High Sensitivity (Agilent, evaluates library size), Qubit (Thermo Fisher, evaluates dsDNA quantity),  and KAPA qPCR analysis (KAPA Biosystems, evaluates quantity of amplifiable transcript). Samples were mixed in equimolar fashion and sequenced on an Illumina HiSeq 2500 rapid run mode according to the standard 10X Genomics protocol. TCR target enrichment, 5' gene expression library, and TCR library were carried out to the 5' Chromium Single Cell V(D)J Reagent Kit manual (10x Genomics).