ATAC-seq of Th17 cells
Briefly, 50k cells were transposed with Illumina Tagment DNA Enzyme (TDE1, 20034198). Library amplification was completed with 2X Phusion High Fidelity Mastermix (Thermo Scientific, F531L) with Illumina RNA/DNA UD indexes. Library quantification and assessment was done using a Qubit FLEX fluorometer and an Agilent TapeStation 4150. Libraries were normalized and pooled to 2 nM by calculating the concentration based off the fragment size (base pairs) and the concentration (ng/l) of the libraries. Sequencing was performed on an Illumina NextSeq 2000, using a P2 100 flow cell. The pooled library was loaded at 750 pM and sequencing was carried out with read lengths of 2x58 bp, with a target of 50 million reads per sample.