Whole genome DNA sequencing of D. pachys
D. pachys PF1309 DNA was extracted using the MagAttract HMW DNA kit from Qiagen (Hilden, Germany) per the manufacturers instructions. Genomic DNA library was constructed using the DNA ligation kit from Oxford Nanopore Technologies (Oxford, UK) following the manufacturers instructions. The library was sequenced on a Oxford Nanopore R9.4.1 flowcell (FLO-MIN106) and basecalled using Guppy (v 4.0.11).