OmniC Sequencing for Lycorma delicatula
Chromatin was fixed with formaldehyde prior to digestion with DNAse I. Chromatin ends were repaired and ligated to a biotinylated bridge adaptor before proximity ligation. After reverse crosslinking, the DNA was purified and treated to remove unligated biotin. NEBNext Ultra enzymes and Illumina-compatible adaptors were used to construct sequencing libraries before biotin-containing fragments were extracted with streptavidin beads and enriched via PCR. The Omni-C libraries were sequenced on an Illumina HiSeq X to ~30x coverage resulting in 95.6 Gb of Hi-C data.