GSM8800015: hiPSC-CM-D16, sample 1; Homo sapiens; RNA-Seq
iPSC-CMs were trypsinized into single cells, fixed with fixation and permeabilization solution (51-2090KZ) for 30 minutes at 4 °C, and blocked in Human BD Fc Block (564219) at room temperature for 10 minutes. Cells were then incubated with primary antibodies and isotype control antibodies at room temperature for 40 minutes, incubated with corresponding fluorescently conjugated secondary antibodies for 30 minutes, and resuspended in wash buffer (554723). Flow cytometry was performed with an LSR Fortessa instrument (BD Biosciences, USA). All cell lines RNA sequencing was performed by the Heflin Center for Genomic Sciences, the University of Alabama at Birmingham, using Illumina NextSeq500 platforms RNA quality control was achieved by removing reads containing adapters, having more than 10% of undetermined bases, and low-quality reads. Less than 2% of these reads were removed in all samples.