NPUS1
The RNA library was prepared using the Novogene NGS RNA Library Prep Set (PT042) according to the manufacturer's protocol. Briefly, mRNA was fragmented randomly, and cDNA was synthesized using random hexamers. After second-strand synthesis, the double-stranded cDNA underwent end repair, A-tailing, adapter ligation, and PCR enrichment. The qualified libraries were sequenced on an Illumina platform  with paired-end 150 bp reads