GSM9115952: ROD_38; Homo sapiens; RNA-Seq
RNA isolation was performed with the RNeasy Mini Plus kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol. RNA from lysed and homogenized cells was purified by a column-based system and washed several times before elution with 30 µl RNase-free ddH2O. Samples were stored at -80°C for further processing. The RNA sequencing library was prepared with Illumina Stranded mRNA Prep (Illumina, San Diego, California, USA) and IDT for Illumina DNA/RNA UD Indexes Set A (Illumina). The purified samples were sequenced in the Illumina NovaSeq 6000 with the NovaSeq 6000S2 reagent kit (338 cycles, paired end 2 x 159 bp) with an average read of 51.8 M reads per RNA sample.