RNA-Seq of Homo Sapiens: donor 2 Tregs-IL2pa
RNA library preparation were realized following manufacturers recommendations (NEBnext rRNA depletion #E6310 + Ultra II Directional RNA kit #E7760L from New England Biolabs). Final samples pooled library prep were sequenced on ILLUMINA novaseq 6000 with SP-200 cartridge (2x800Millions of 100 bases reads), corresponding to 2x30Millions of reads per sample after demultiplexing.