Illumina NovaSeq X Plus paired end sequencing
Paired-end 150 bp RNA-seq of PBMCs. Libraries sequenced on Illumina NovaSeq X Plus targeting approximately 6 Gb per sample.
Total RNA extracted with RNeasy Mini Kit (Qiagen). Libraries prepared by Novogene using the Novogene NGS RNA Library Prep Set (PT042), including end repair, A-tailing, adaptor ligation, size selection, amplification and purification.