RUSB22 'Newton' leaf - 440bp library
The 440bp library was prepared with the Hyper Kapa Library Preparation kit (Kapa Biosystems) with no PCR-amplification. The 440bp library were quantitated by qPCR and sequenced on one lanes for 251 cycles from each end of the fragments on a NovaSeq 6000 using a NovaSeq SP reagent kit. Fastq files were generated and demultiplexed with the bcl2fastq v2.20 Conversion Software (Illumina).