Ampliconseq of 16S V4 rRNA markers: early successional plant community soil
Bacterial communities in soil were profiled using amplicon sequencing of the 16S rRNA V4 region. The V4 region of the 16S rRNA gene was amplified using Unique Dual Indexed, Illuminacompatible primers following Kozich et al. (2013), with forward primer 515f (5GTGCCAGCMGCCGCGGTAA3) and reverse primer 806r (5GGACTACHVGGGTWTCTAAT3).