SF240729HJY013 (CRX1909826; CRA028959)
The concentrated water (250 ul) was used for metagenomic DNA extraction. The genomic DNA was purified and isolated with MagaBio Soil/Feces Genomic DNA Purification Kit. The extracted DNA was quantified at an A260/A280 nm ratio with the NanoDrop ND-1000. Then extraction from each sample was diluted to approximately 5ng/ul and stored at -20 C for metagenomic next-generation sequencing. The PCR products were subjected to 1% agarose gel electrophoresis and then sequenced on an Illumina (PE 345) platform of Honsunbio company