Diaporthe species genome sequencing
Agarose gel electrophoresis was used to analyze DNA for purity and integrity. After the DNA sample is qualified, it is randomly interrupted by the Covaris ultrasonic breaker, and then the entire library preparation is completed by the steps of end repair, A-tail, sequencing adapter, purification, and PCR amplification. After the library was constructed, Qubit 2.0 was used for initial quantification, the library was diluted, and then the inserts of the library were detected using the Agilent 2100, and after the insert size met the expectations, the effective concentration of the library was accurately quantified using the Q-PCR method to ensure the quality of the library. After the library was qualified, different libraries were pooled to flowcell according to the effective concentration and target data volume, and the cBOT was clustered and sequenced using the Illumina high-throughput sequencing platform NovaSeq 6000