sequences of bacteria
16S rRNA gene V3-V4 region amplicon sequencing. Primers: 341F (CCTACGGGNGGCWGCAG) and 806R (GACTACHVGGGTATCTAATCC). PCR conditions: 95C 3min, 25-30 cycles (95C 30s, 55C 30s, 72C 30s), 72C 5min. Library prepared using NEBNext Ultra II FS DNA Library Prep Kit. 300 bp paired-end sequencing.