GSM9068673: si-CHAF1A_1_CUT&Tag; Homo sapiens; OTHER
Briefly, 1 × 10⁶ cells were dissociated into single cell for use. Then cells were mixed with ConA Beads and incubated with primary antibody overnight at 4°C. After incubating with secondary antibody, and pA-Tn5 Transposon, DNA was tagmentated by Tagmentation buffer and extracted by VAHTS DNA Clean Beads (Vazyme). Cells were transfected with si-CHAF1A for 48 hours Then, cells per group were collected for CUT&Tag assays using the Hyperactive Universal CUT&Tag Assay Kit (TD904-01, Vazyme Biotech, Nanjing, China).