GSM9696235: HT-1080 ATF3-OE _APR-246 rep3; Homo sapiens; RNA-Seq
RNA was extracted using TRIzol (Invitrogen) and purified with Dynabeads Oligo (dT)25 (Thermo Fisher) for mRNA enrichment. Fragmentation was conducted at 94°C for 5–7 min. Reverse transcription of RNA to cDNA was accomplished using SuperScript II. DNA polymerase I, dUTP and RNase H were used for second strand synthesis. Following end repair, A-tailing, and ligation of adapters with T-overhangs, fragment selection was completed using AMPure XP beads. The library was amplified with PCR (fragment sizes approx. 300 ± 50 bp).