s-Control
Plants were subjected to 5 mM H2O2 stress for 2h. The root apex (05 mm from the tip) and mature (~20 mm) root zones was then harvested. Total RNA was isolated using a TRIzol kit (Invitrogen, Carlsbad, CA, USA) with three biological replicates for each treatment. Purity, concentration and integrity of RNA sample were examined by NanoDrop, Qubit 2.0, Agilent 2100 (Agilent Technologies, CA, USA). Library construction and RNA sequencing (RNA-seq) were conducted by the BMKgene Bioinformatics Institute (Beijing, China) using the high-throughput sequencing platform