GSM9035606: CD8 cells, NaCl treatment, DNA replicate 1; Mus musculus; ATAC-seq
Total RNA was isolated via PureLink RNA Mini Kit (Invitrogen). RNA-seq libraries were generated from Poly(A) RNA capture using the Illumina TruSeq RNA Sample preparation kit v2. ATAC-seq libraries were prepared from approximately 100,000 T cells via Tn5 transposition with digitonin at 37 °C for 30 minutes, followed by DNA purification and 8–10 cycles of PCR amplification.