Short Read-seq of E. khazarica
DNA was extracted from pure cultures using the Wizard Genomic DNA Purification Kit. Libraries were prepared with a Nextera XT Kit (Illumina, California, EUA), purified with Agencourt AMPure XP beads (Beckman Coulter, California, USA), and quantified by qPCR (KAPA Fast Universal kit - Illumina/Universal). Whole-genome sequencing was then performed on the Illumina NextSeq 500 System, with the NextSeq 500 MID Output kit (300 cycles).