GSM8972655: LN remission.01; Homo sapiens; RNA-Seq
To isolate RNA from cell cultures, the RNeasy Mini Kit (Qiagen, Hilden, Germany, #74104) was used. Cells were directly lysed by adding Buffer RLT while scraping the cells. RNA was then isolated according to the manufacturer's protocol. RNA was immediately placed on ice for subsequent analysis. RNA purity and concentration were assessed using a Nanodrop spectrophotometer, ensuring an OD 260/280 ratio ≥ 2.0 and an OD 260/230 ratio ≥ 2.0 for RNA sequencing quality. Libray preparation and sequencing conducted by Novogene. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis.