Visium HD spatial transcriptomics of WT intragingival A. actinomycetemcomitans EV-injected mouse brain sample 1
10x Genomics Visium HD spatial transcriptomic library prepared from FFPE mouse brain tissue using the Visium HD Spatial Gene Expression for FFPE library v1 - Mouse (6.5 mm) kit and sequenced on an Illumina NovaSeq 6000 platform by Macrogen. Raw FASTQ files are submitted to SRA; downstream spatial transcriptomic processing was performed using the standard Visium HD binned-output workflow. Main dataset; mouse received intragingival injections of Aggregatibacter actinomycetemcomitans-derived extracellular vesicles into the interdental gingiva between the maxillary molars twice weekly for 12 weeks; injections started on 2025-04-21; brain tissue was collected on 2025-07-14.