GSM9084880: Control; Homo sapiens; RNA-Seq
Cells were disociated with accutase 5min at 37C and resuspended in 0,04%BSA-PBS 1x. RNA libraries were prepared using standard 10X Genomics 3' Single Cell v3.1 protocol. Cells loaded to Chromium Chip (target cell recovery): 6,000 cells. Library Preparation Kit: Chromium Next GEM Single Cell 3ʹ Reagent Kit v3.1 (10x Genomics). Sequencing parameters: NovaSeq 6000, SP Flow Cell, 100 cycles. Read 1: 28 bp, i7-index: 10 bp, i5-index 10 bp, Read 2: 90 bp.