Non-tumorous adjacent tissue-032
Circle-seq: DNA from all tissue samples (6 mg) were extracted with MagAttract HMW DNA Kit (Qiagen, Germany) as described in the manufacturer`s protocolFor the eccDNA sequencing, a portion of each 29-amplified DNA sample was diluted to 15 ng/L in 10 mM Tris-HCl and the libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina (NEB, USA) in accordance with the manufacturer's protocol