Full-length 19S rRNA amplicon of gill-associated bacterial microbiome from Idas sp.
Total DNA extracted from gill tissue of Bathymodiolinae sp. was used to amplify the full-length bacterial 16S rRNA gene with barcoded universal primers 27F (AGRGTTYGATYMTGGCTCAG) and 1492R (RGYTACCTTGTTACGACTT). PCR was performed in triplicate, amplicons were purified and quantified, libraries were prepared with the SMRTbell Prep Kit 3.0, and sequencing was carried out on the PacBio Sequel IIe System. HiFi reads were generated from subreads using CCS mode in SMRT Link v11.3.