PBMC RNAseq of Linezolid treated TB patients
Total RNA was extracted from PBMCs using the PAXgene Blood RNA Kit (TIANGEN, Beijing, China) following the manufacturers instructions. mRNA libraries were prepared using the Optimal Dual-mode mRNA Library Prep Kit (BGI, Shenzhen, China). Briefly, mRNA was enriched using oligo(dT) magnetic beads, fragmented, and reverse-transcribed into cDNA. After end repair, A-tailing, and adaptor ligation, libraries were PCR-amplified and quality-checked. Circularized single-stranded DNA libraries were generated and amplified by rolling circle amplification to form DNA nanoballs (DNBs), which were sequenced using the BGI platform with 150 bp paired-end reads.