GSM8868983: Cbfb-1_CUTnRUN; Mus musculus; OTHER
Single-cell suspensions of postnatal day 6 Nanos2-Cre and Rosa26-tdTomatofl/+ testes were prepared and germ cells isolated using fluorescence activated cell sorting by gating for tdTomato+ cells. Next, cells were lysed to isolate nuclei following the manufacturer recommendations and CUT&RUN was performed using the CUTANA™ ChIC/CUT&RUN Kit (EpiCypher 14-1048, v.3.5). Approximately 1 μg of antibody was used for each CUT&RUN reaction, including a rabbit anti- CBF (Ichiro Taniuchi), rabbit anti-RUNX1 (Abcam, ab23980), rabbit anti-RUNX3 (Cell Signaling Technology, 9647), H3K4me3 (EpiCypher; positive control), and IgG (EpiCypher; negative control). Genomic libraries were generated using CUTANA™ CUT&RUN Library Prep Kit (EpiCypher, v.1.3).