MinION sinlge-end Sequencing of KAS22022583 (KAE22245650)
Forsythia koreana genome sequencing for CP genome extraction
The Rapid PCR Barcoding Kit offers the fastest and simplest method of preparation of barcoded libraries for low quantities of gDNA 1-5 ng, with only ~15 mins of hands-on preparation time.  At the heart of the kit is a transposase which simultaneously cleaves template molecules in each sample and attaches tags, which contain primer binding sites, to the cleaved ends. The kit contains 12x primers which are then used to amplify each sample: each primer contains a barcode and 5??tag which facilitates the ligase-free attachment of Rapid Sequencing Adapters. Amplified and barcoded samples are then pooled together and Rapid Sequencing Adapters are added to the pooled mix.