GSM8698402: ∆PWS iPSC VP64 NT gRNA rep1; Homo sapiens; RNA-Seq
14 days after gRNA virus transduction, cells were harvested with Accutase, pelleted, and total RNA was extracted using Norgen Total RNA Plus Micro kit. Total RNA was submitted to Genewiz (Azenta) for library prep and total RNA sequencing. RNA libraries were prepared by Genewiz/Azenta Life Sciences. Total RNA library was constructed with rRNA depletion method.