GSM9712495: Retinal Ganglion Cells, 2wks post ONC, scramble shRNA, rep 1; Mus musculus; RNA-Seq
Approximately, 5,000 RGCs (for each of the 2 replicates per condition) were collected by FACS and RNA was isolated immediately using the Quick-RNA Microprep Kit (ZR1050, Zymo Research) cDNA libraries were prepared using polyA-selected RNA (TruSeq RNA Library Prep Kit, Illumina)