Illumina MiSeq sinlge-end Sequencing of KAS22050726 (KAE22340310)
20 mice are purchased from two vendors (Koatech, Orientbio). Each vendor fecal sample was mixed with PBS (pH 7.4) and homogenized using zirconium oxide beads by vortexing. Then, supernatants were preserved in 60% w/v glycerol. All mice were fed with water containing antibiotics mixture (0.5 g/L vancomycin, 1g/L neomycin, 1g/L metronidazole, 1 g/L ampicillin, 1 g/L streptomycin) for 14 days. 20 mice from Koatech (K-FMT) were divided into two groups and each vendor fecal sample was introduced into each group (K-FMT-K, K-FMT-O; The last initial means the vendor origin of the fecal sample). by oral gavage (200 ul per mouse, once a day, for 2 days). The same goes for 20 mice from Orientbio (O-FMT-K, O-FMT-O). After 5 days, fecal samples from 4 groups were collected and DNA was extracted using Maxwell RSC PureFood GMO and Authentication kit (Promega).
Nextera XT DNA Library Preparation Kit FC-1-2-1-26