GSM9696280: snRNA-seq mouse brain pooled run L003; Mus musculus; RNA-Seq
Nuclei were isolated from mouse brain tissue using mechanical dissociation and filtration. Isolated nuclei were resuspended in buffer for downstream single-nucleus RNA sequencing. Single-nucleus RNA-seq libraries were generated using the inDrops platform. Individual nuclei were encapsulated into droplets and barcoded using hydrogel beads. Reverse transcription was performed within droplets, followed by cDNA amplification and library preparation for Illumina sequencing.