GSM8734426: Cal101-3, scRNA-seq; Mus musculus; RNA-Seq
Half tumors were resected from the skin, manually dissociated, processed with collagenase IV solution, then sorted for Live CD45.2+ cells on a BD FacsAria Fusion cell sorter. Cells were then incubated with the Total-Seq-B Mouse Universal Cocktail (BioLegend) Chromium Single Cell NextGEM 3' v3.1 kit with Feature Barcoding technology for Cell Surface Protein (10x Genomics, CG000390)