GSM9326027: Ventricular fibroblasts, DMSO, rep1; Mus musculus; Hi-C
Following cell collection, genomic DNA was isolated, crosslinked, and digested with a proprietary restriction enzyme cocktail. Digested DNA overhangs were filled with biotin-14-dATP, and the resulting blunt ends were ligated to capture proximity interactions. The ligated DNA was then purified, sonicated, and size-selected, followed by streptavidin-based enrichment to capture biotinylated ligation junctions. Final Hi-C libraries were constructed using distinct Illumina indexed adapters and subjected to paired-end sequencing