Shotgun DNA sequencing of activated sludge: line 9 sample C
DNA was extracted from the biomass with the DNeasy PowerSoil Pro Kit (Qiagen). The DNA samples underwent general DNBseq DNA library preparation and PE150 sequencing. Adapters, barcodes and low-quality reads (<Q20) were removed by the sequencing provider using the SOAPnuke pipeline (version 1.5.5).