RNASeq of RALF-FER-LRX-Mutants: Set1_lrx5xuntr1dpi
Whole leaves were harevested from adult plants and directly frozen in liquid nitrogen. RNA was extracted using the TRIzol reagent (Roche, Switzerland) and purification with Direct-zol RNA Miniprep Plus kit (Zymo Research, Germany) with on column DNAse I digestion. RNA quality was and quantity was confirmed using gel-electrophoresis, nanotrop and qubit measurement. cDNA library was generated using 3 mRNA-Seq V2 Library Prep Kit FWD with Unique Dual Indices (12nt).