RNA-seq of Sulfolobus islandicus M.16.4 In vitro exponential growth replicate 3
The hyperthermophilic archaeon S. islandicus M.16.4 was cultivated aerobically in Tissue Culture Flasks (Fisher Scientific, USA) at 76-78C and pH 3.5 without shaking in DT medium , which is comprised of a basal salt medium supplemented with 0.2% (w/v) Dextrin (Sigma-Aldrich, USA) and 0.1% (w/v) Ezmix N-Z-Amine A (Sigma-Aldrich, USA). Exponentially growing cells were diluted to an OD600 of 0.01 in a 130 ml DT medium and their growth was monitored over an 8-day incubation period by measuring OD600