Mcav_diseased_D12_metagenome
Diseased and healthy tissue samples were collected from 4 colonies of Montastraea cavernosa exhibiting SCTLD symptoms. The collection was conducted at patch reefs sites near Mote Marine Laboratory, Florida Keys. . DNA and RNA extractions were carried out using the ZymoBIOMICS DNA-RNA Miniprep Kit (Zymo Research) following the manufacturer's protocol. Coral tissue homogenization was achieved using ceramic and glass beads (Lysing Matrix A, MP Biomedicals) and a bead beater. After extraction, total RNA and DNA were quantified spectrophotometrically using the DeNovix. Metatranscriptomic libraries from eight RNA samples (four healthy and four diseased) were prepared using Universal Prokaryotic RNA-seq, Any-Deplete kit (NuGen). In addition, four metagenomic libraries were prepared from the DNA of the diseased tissue using the NEBNext DNA Library prep kit (New England Biolabs).