GSM8490615: Healthy CNS; Mus musculus; RNA-Seq
T-ALL and myeloid cells were FACS-sorted (CD11b+ and/or CD11c+, myeloid; CD45.1-CD45.2+CD5+, T-ALL) from the CNS of healthy and leukemic mice following cardiac perfusion. Single-cell suspensions were prepared from isolated hematopoetic cells (Percoll gradient separation) and stained for FACS sorting prior to scRNAseq. Sorted cells were collected in cRPMI without EDTA and washed into FWB prior resuspension at a concentration of 700-1,200 cells/microliter. Cells were submitted to UT-Austin's GSAF for scRNA-seq analysis. Cell suspension were processed for cDNA library generation following manufacturer instructions (10X Genomics) for use with the single cells 3' ragent kit v3.1 (10X). Samples were sequenced using the Illumina NovaSeq platform (SR100 S1 Run type) at read depth of 50,000 reads per cells.