GSM9837082: TGFB1-overexpressing lens epithelium (OVE918) replicate 3; Mus musculus; RNA-Seq
For OVE853 mice, three biological replicates were collected for RNA extraction and sequencing, with each replicate consisting of up to 18 freshly isolated lens epithelial explants from 9 mice exhibiting anterior subcapsular cataract plaques. For OVE918 mice, four biological replicates were collected, with each replicate consisting of up to 14 freshly isolated lens epithelial explants from 7 mice. For WT controls, three biological replicates were collected, each consisting of lens epithelial tissues pooled from up to 9 mice. Tissues for each replicate were immediately pooled in 500 µL TRIzol reagent and stored at -80°C. Total RNA was extracted according to the manufacturer's instructions. RNA samples (2.5 µg) were treated with TURBO DNA-free Kit (Invitrogen) to remove genomic DNA contamination. RNA quantity and quality were assessed using the RNA 6000 Nano Kit on an Agilent Bioanalyzer. Samples with RNA Integrity Number (RIN) values greater than 7.0 were used for sequencing. mRNA sequencing libraries were prepared according to the manufacturer's protocol.