GSM8869309: F0 dancr crispant (2); Danio rerio; RNA-Seq
Zebrafish embryos were homogenised using TRIzol and total RNA was isolated using the Quick-RNA Microprep Kit (Zymo Research). Residual genomic DNA was then removed using DNA-free™ DNA Removal Kit (ThermoFisher Scientific) polyA RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP for strand specific library.