scRNA-Seq of mus musculus: 8 week-old tumor-bearing brain
Samples were comprised 2 male and 2 female biological replicates pooled together. Tumor-bearing right hemisphere of mus musculus brain was dissociated with Miltenyi Multi-Tissue Dissociation Kit and GentlMACs. Red blood cells were lysed, and dead cells were removed using Miltenyi Dead Cell Removal Kit. 10x Genomics 3' GEX libraries were prepared using the single cells, and subseuqently analyzed with an Illumina NovaSeq X Plus, at 2x 150.