Genome sequencing of Pristis pristis: dried rostrum sample)
Libraries were prepared using the Illumina Nextera protocol with 20 ng genomic DNA and tagmentation for 2025 minutes, followed by fragment size analysis on a Tapestation 4200. Sequencing was performed on the Illumina NextSeq 2000 platform, generating 300 bp paired-end reads with an insert size of 459526 bp, achieving ~6x genome coverage.