GSM8768148: FFPE tissue, DCIS 17; Homo sapiens; RNA-Seq
Blood samples were collected into CellRescue tubes (Menarini) and processed on the microfluidic Labyrinth system. Downstream, the enriched CTCs were supplemented with additional white blood cells until the cell count reached ~ 800,000 total cells. Then, these cells were used as input into 10X Genomics' Flex Protocol. At the end of the protocol, cells were frozen at -80C with Glycerol and Thaw Enhancer following the protocol's recommendation. Frozen samples were submitted to the university's Genomics core for processing on the 10X Chromium system. The library construction was performed by the University of Michigan's Advanced Genomics Core and in accordance with 10X Genomics' Flex/Chromium Fixed RNA profiling protocol.