GSM8948084: ECRS_Polyp_1; Homo sapiens; RNA-Seq
Single-cell suspensions were prepared from mouse lung tissues by isolating CD4(iv)-negative tissue-resident and CD4(iv)-positive circulating CD4⁺ T cells via FACS (BD FACS Aria III), with 10,000 viable cells (>98% viability) collected per subset; human nasal polyps were finely minced and enzymatically digested in RPMI 1640 containing collagenase III, hyaluronidase, and DNase I, followed by mononuclear cell isolation through Percoll density gradient centrifugation and 70-μm nylon mesh filtration, while peripheral blood mononuclear cells were isolated using Ficoll-Paque Plus and similarly filtered to ensure high-quality single-cell suspensions. Single-cell RNA-seq libraries for both mouse and human (nasal polyps and PBMCs) samples were generated using the Chromium Single Cell 3' Reagent Kits v3.1 with dual indexing (10x Genomics), and single-cell ATAC-seq libraries for mouse samples were prepared using the Chromium Next GEM Single Cell ATAC Reagent Kits v1.1 (10x Genomics), followed by sequencing on a NovaSeq 6000 system (Illumina) with appropriate read settings and achieving a minimum depth of 20,000–25,000 read pairs per cell or nucleus according to assay type.