ITS metabarcoding of Agrilus planipennis
DNA was extracted from ash tree and emerald ash borer samples using Qiagen PowerSoil Kit. The DNA was stored at -20 C until sequencing. All amplicon library prep and sequencing of fungal ITS1 (ITS1F-Nextera, ITS2-Nextera) and bacterial 16S V3-V4 region (357F-Nextera, 806R-Nextera) were performed at the University of Minnesota Genomics Center.