GSM9396393: 7d post-Sx, bio rep 4; Homo sapiens; RNA-Seq
Approximately 20mg of quadriceps muscle was homogenized in TRI Reagent (#R2050-1-200, Zymo Research, Orange, CA) using Zirconium Oxide beads (2.0 mm, RNase-free, Next Advance, Troy, NY) and a bullet blender (Next Advance Bullet Blender Storm, Next Advance). RNA was isolated using the Direct-zol RNA Miniprep kit (#R2051, Zymo Research) per manufacturer's instructions. RNA concentration and quality (RIN > 8.0, average RIN: 8.7) was assessed with an RNA Nano chip kit (#5067-1511, Agilent, Santa Clara, CA) using the 2100 Bioanalyzer (Agilent) at the University of Kentucky Genomics Core Laboratory. Six hundred nanograms of total RNA was sent to Novogene (Chula Vista, CA) for library construction and sequencing on an Illumina NovaSeq 6000 system using a paired-end 150 bp dual-indexing protocol.