GSM8521384: 93_WT_0_rep1; Streptococcus mitis; RNA-Seq
RNA was extracted using the Quick-RNA Fungal/Bacterial Miniprep Kit (Zymo Research, Freiburg, Germany) according to the manufacturer's protocol. Genomic DNA contamination was eliminated using the TURBO DNase Kit (Thermo Fisher Scientific, Waltham, MA, USA). The RNA was further purified and concentrated using the RNAClean XP Reagent (Beckman Coulter, Brea, CA, USA). RNA quality and quantity were assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). High-quality RNA samples were reverse transcribed into cDNA using the Ion Torrent™ NGS Reverse Transcription Kit (Thermo Fisher Scientific). cDNA libraries were constructed with the Ion Torrent™ Ion Total RNA-Seq Kit v2, incorporating adapters from the Ion Torrent™ Ion Xpress™ Barcode Adapters 1-96 Kit for multiplexing. Library amplification and template preparation were performed using the Ion 550™ Kit on an IonChef system. Libraries were loaded onto four Ion 550 chips for sequencing on an Ion GeneStudio™ S5 Plus system (Thermo Fisher Scientific).