RNA-seq of Homo sapiens:sh_MARK2_C
RNA-Seq were prepared, sequenced and analyzed by Novegene (Beijing, China). Briefly, total amounts and integrity of RNA were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). After total RNA was extracted, mRNA was isolated by Oligo Magnetic Beads and fragmentation was carried out using divalent cations under elevated temperature. First strand cDNA was synthesized using random primer and Reverse Transcriptase. Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and dNTP. After adenylation of 3 ends of DNA fragments, Adaptor with hairpin loop structure were ligated to prepare for hybridization. In order to select cDNA fragments of preferentially 370~420 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). After PCR amplification, the PCR product was purified by AMPure XP beads to obtain finally library. Libraries were tested and sequenced by the Illumina NovaSeq 6000.