1.1.7
prokaryotic library preparation , fragment of the V4 region of the bacterial 16S rRNA gene of around 290 bp was amplified using the primers 515F (5GTG YCA GCM GCC GCG GTA A 3) (modified from Parada et al. (2016) and 806R (5GGA CTA CNV GGG TWT CTA AT 3) (modified from Apprill et al. (2015).