Metatranscriptome sequencing California Costal Environment
RNA was extracted using the Macherey-Nagel NucleoMag RNA kit (Cat. No. 744350) on an Eppendorf epMotion 5075t as described here: dx.doi.org/10.17504/protocols.io.bd9ti96n.  PolyA-enriched RNA and Poly A library were prepared from total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (for 96 samples) kit (New England Biolabs; Catalog# E7490L) and the NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads kit (Catalog# E7765L).