RNA seq of mouse striatum (YAC128) - high amiR136-13A replicate 2
Total RNA was isolated from mice striata. Briefly, 3 and 5 adaptors were ligated to 3 and 5 end of small RNA, respectively. Then the first strand cDNA was synthesized after hybridazition with reverse transcription primer. The double-stranded cDNA library was generated through PCR enrichment. After purification and size selection, libraries with insertions between 18~40 bp were ready for sequencing with SE50.