Raw unaligned PBMC scRNAseq data
Single-cell (PBMC) suspensions were stored in liquid nitrogen until processing. Upon thawing, cell viability and concentration were assessed using the Nexcelom Cellaca Cell Counter according to manufacturer protocols. Samples were diluted to the appropriate concentration and loaded onto the 10X Genomics Chromium Controller to target the capture of approximately 10,000 gel bead-in-emulsions (GEMs) per sample. Single-cell libraries were prepared using the 10X Genomics Chromium Single Cell 3' v4 chemistry kit, following the manufacturer's standard protocol. Sequencing libraries were assessed for quality on an Agilent TapeStation and quantified using both a Qubit 2.0 Fluorometer (Thermo Fisher Scientific) and qPCR (Applied Biosystems). Final libraries were sequenced on an Illumina NovaSeq X Plus instrument using a paired-end configuration of 28 × 91 bp reads, in accordance with 10X Genomics recommendations. Sequencing aimed for a depth of 50,000 reads per cell.