RNA-seq PA3321 Rep 1
The mutant PA3321::Tn and the wt PA01 strain were grown overnight in LB broth, diluted 100-fold into fresh LB broth, and grown in the same biofilm growth condition (30oC, no shake, 48 hours), as described above. Bacteria were harvested, and total RNA was isolated using the Qiagen RNeasy Mini Kit (Qiagen), with on-column DNase I treatment; rRNA was depleted using RiboZero (Epicentre); and Illumina NGS libraries were created and sequenced at TUCF Genomics (http://tucf-genomics.tufts.edu/).