amplicon sequencing of influenza A: UVM-1927
Extraction performed using QIAmp Viral RNA Mini Kit (Qiagen #52906). Then amplification performed using Uni/Inf primer set (10.1007/978-1-61779-621-0_11) and Superscript III high-fidelity RT-PCR kit (Invitrogen #12574-035) and then confirmed by visualization of 7-8 bands on agarose gel. Samples were then purified using QIAquick PCR Purification kit (Qiagen #29104). Samples were then sent to Emory EPC Genomics core who performed Illumina NexteraXT DNA library prep and Illumina based amplicon sequencing.