RUSB22 'Newton' leaf - 10X Genomics Chromium
The 10X libraries were constructed with the Chromium Genome Library Kit & Gel Bead Kit v2 (10X Genomics). The 10X and MP libraries were quantitated by qPCR and sequenced on two lanes for 151 cycles from each end of the fragments on a NovaSeq 6000 using a NovaSeq S4 reagent kit. Fastq files were generated and demultiplexed with the bcl2fastq v2.20 Conversion Software (Illumina) .