GSM8490241: WT_Mock rep 2; Mus musculus; RNA-Seq
Neutrophils were magnetically sorted from pooled BALF from mock, single (Af, IAV), and IAV-Af co-infected mice at 48 hours after single/co-infection. RNA extraction was performed using Qiagen RNA extraction kit. Libraries were prepared with a NEBNext Ultra II RNA-Seq library kit following the manufacturer's instructions as described earlier. DNA and RNA quantity was assessed using a Qubit fluorometer, and the quality was evaluated using a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). Illumina TruSeq mRNA Sample Prep v2 kit (Catalog #s RS‐122‐2001, RS‐122‐2002; Illumina, San Diego, CA, USA) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols