GSM9069610: Induced wild type Sp1 line, anti-FLAG, replicate 1; Homo sapiens; OTHER
CUT&Run was performed as previously described (dx.doi.org/10.17504/protocols.io.zcpf2vn), using 0.5x10^6 cells per antibody, 0.02% digitonin and pA/G-MNase digestion (30 minutes on ice). After phenol:chloroform extraction DNA was purified with home-made serapure beads (Doi:10.1101/gr.128124.111). NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®, adapted for short fragments (dx.doi.org/10.17504/protocols.io.bagaibse); adaptors diluted 1:20; 11 PCR cycles