ITS2 sequencing of ATBC attached soil
Amplicons purified using an 18% suspension of Sera-Mag Speed-beads Carboxyl Magnetic Beads (GE Healthcare) were subjected to dual indexing using the Nextera XT Index Kit (Illumina) as per the manufacturers instructions.The Illumina-indexed pools were purified using an 18% suspension of Sera-Mag Speed-beads Carboxyl Magnetic Beads (GE Healthcare) as described above. Sequencing was performed on an Illumina MiSeq with a MiSeq Reagent Kit v3 600 cycles (Illumina) using 8pM libraries spiked with 30% PhiX Control v3 (Illumina) according to the manufacture's instructions.