16S rRNA sequencing of Iphiseius degenerans at low and sub-zero temperatures
DNA was extracted using a DNeasy Blood and Tissue Kit (Qiagen) following the manufacturers standard protocols. Samples were sequenced on the Sequel IIe system by PacBio. Raw sub-reads were processed through the SMRTlink (v11.0) Circular Consensus Sequences (CCS) algorithm to produce highly accurate reads (>QV40). These highly accurate reads were then processed through Vsearch and taxonomic information was determined based on QIMME2