1_P2F10W1
Total RNA was purified with the EZ2 Connect instrument (Qiagen) using the EZ2 RNA/miRNA Tissue/Cells Kit (Qiagen). RNA extracts were sent to Azenta Life Sciences for rRNA depletion, library construction, and sequencing. Dual depletion of bacterial and animal rRNA was conducted using the NEBNext rRNA (Bacteria and Human/Mouse/Rat) Depletion Kits (New England Biolabs), respectively. Truseq 2x150 bp libraries were prepared and sequenced on a NovaSeq sequencing instrument (Illumina)