II_MX221002 (CRX1174544; CRA018585)
For Illumina sequencing, a library with a short-fragment size of 350 bp was prepared using the Covaris ultrasonicator. The DNA fragments were further treated with end repair, A-tailing, adapter ligation, purification, and PCR amplification. Then the library was subjected to sequencing on an Illumina NovaSeq 6000 platform (Illumina, USA) to generate 150-bp paired-end (PE150) reads.