GSM9325917: wt rep2; Caenorhabditis elegans; RNA-Seq
Young adult worms were lysed using mirVana Kit (Ambion, Cat #1560 ) following manufacturer´s instructions. Homogenization of the lysate was performed using a conventional rotor-stator homogenizer polytron, pre-chilled with liquid nitrogen. TruSeq Stranded mRNA Library Prep Kit (Illumina), following the manufacturer's protocol.