16S rRNA V4-V5 amplicon sequencing of soil sample BDK_SP3L_R2_WCL1d
The V4V5 region of the 16S rRNA gene was amplified using the 515F926R primer pair (Parada et al., 2016), which included partial Illumina adapter sequences. Amplicons were subsequently subjected to an indexing PCR to attach sample-specific barcodes. Libraries were pooled and sequenced on an Illumina NovaSeq platform using 2  250 bp paired-end chemistry.