Amplicon metabarcoding: marine metagenome sample
We sampled eDNA from surface and bottom samples across 4 transects with 4 stations each across the Eastern Shore Islands Area of Interest, an area of approximately 2000 km^2 in coastal Nova Scotia. In 2023 however, only one transect was sampled (ESI_05 through ESI_08) in spring, summer, and fall to collect seasonal samples. Using a CTD rosette (SeaBird) we collected water samples from the ocean and filtered these on 1.2 micron Smith-Root filters to capture eDNA. 12S and COI amplicons were sequenced in 2023 on a MiSeq instrument at the Aquatic Biotechnology Laboratory, Dartmouth, Nova Scotia