GSM8490636: batch3_Ctrl_RNaseH_rep2; Homo sapiens; OTHER
Cells were harvested in NE buffer and then nuclei were collected by ConA beads. The beads were blocked by blocking buffer.  S9.6 antibodies (Abcam, Cat. #ab234957) were added by 1:100 dilution and then secondary antibodies were added to each sample. PA-Tn5 transposase were added to each sample to bind with second antibodies and then the Tn5 were activated by adding MgCl2. This step was used to fragment the genome and add reads to the fragmented DNA. Reactions were stopped by adding stop buffer. 0.9X of VAHTS DNA clean beads were added to each sample to extract the fragmented DNA. For negative control, RNase H were added before the primary antibodies were added. Custom Barcodes Adapters(i5: AATGATACGGCGACCACCGAGATCTACAC-NNNNNNNN-TCGTCGGCAGCGTCAGATGTGTAT; i7: CAAGCAGAAGACGGCATACGAGAT-NNNNNNNN-GTCTCGTGGGCTCGGAGATGTG) were used as primers to add different index to each sample. PCR Master Mix (NEB, Cat. #M0541S) were used to amplify DNA fragments.