!6S amplicon sequencing of human stool metagenome
16S rRNA amplicon sequencing was performed in the the Duchossois Family Institute Microbial Metagenomic facility (DFI-MMF) DNA from stool was extracted using the QIAamp PowerFecal Pro DNA Kit and a V4-V5 region of the 16S rRNA gene was PCR amplified with primers (Fwd-AYTGGGYDTAAAGNG) and (Rev-CCGTCAATTYHTTTRAGT). Illumina compatible libraries were generated using the Qiagen QIASeq 1-step amplicon kit  and sequenced on the Illumina MiSeq platform to generated 2250 PE reads, generating >10,000 reads per sample.