Illumina WGS of phage Simon resistant Bordetella bronchialis isolated from lab
Phage Simon was spotted on a lawn of wt B. bronchialis. Colonies were observed growing in the middle of a phage Simon lysis zone. One of these colonies was purified. It was confirmed to be resistant to phage phage Simon phenotypically and its genome was sequenced. Genomic DNA was extracted from B. bronchialis cultures using the GenFind V3 Reagent Kit (Beckman Coulter) as per manufacturers instructions. Illumina libraries were prepared using the Illumina DNA Library Prep Kit, (M) Tagmentaton (Illumina) and sequenced on the Illumina NovaSeq 6000 platform to produce paired-end reads.