Exome-seq of PaTu-8988T cell line
Genomic DNA was extracted from KRAS KO and intact clones and the parental cell line using the High Pure PCR Template Prep Kit (Roche, 11796828001). Whole-exome sequencing libraries were prepared by the Yale Center for Genome Analysis (YCGA) using the IDT xGen V2 Exome Enrichment Kit and sequenced on the Illumina HiSeq platform (paired-end, 100-bp reads) to a mean target coverage of approximately 30.