flower_2 (CRX701477; CRA010931)
Total RNA was extracted using RNA isolation kit (HuaYueYang Biotechnology, Beijing, China) under the manufacturer's instruction. The cDNA was amplified using the advantage 2 PCR kit (Clontech, CA, USA). The purification was followed by size selection using BluePippin Size selection system (Sage Science, MA, USA) to enrich the fragment longer than 4kb. Then SMRTbell Template Prep kit (Pacific Biosciences, CA, USA) was used to construct a mixed library with those longer than 4kb and the others without selections by the equal molar ratio.  The polymerase-bound template was bound to MagBeads and sequencing was performed on HiSeq 2500 (Illumina) sequencing platform by Novogene company (www.novogene.com).