GSM8870123: Cortical cultures, Fluoxetine 1; Mus musculus; RNA-Seq
Total RNAs were purified from the specimen by thiocyanate-phenol-chloroform extraction method. The process of mRNA purification from total RNA involved the use of poly-T oligo-attached magnetic beads. Subsequent to this, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis. The library was subsequently prepared by performing end repair, A-tailing, adapter ligation, size selection, amplification, and purification. Those process were following manufacturer's protocols.