GSM8972327: WD+CCl4 _Dbi+/+; Mus musculus; OTHER
Tissue samples were initially screened for RNA quality, selecting only those with DV200 scores above 50% (RNA 6000 Nano Kit, Part No. 5067–1511, Agilent, Santa Clara, CA, USA), following the Visium Tissue Preparation Guide (10x Genomics, Pleasanton, CA, USA). Five-micrometer-thick sections were cut from FFPE blocks and mounted on Superfrost Plus slides (VWR, Radnor, PA, USA). Sections were deparaffinized, imaged, and permeabilized (User Guide, CG000520 Rev B) before probe hybridization. Successfully hybridized probe pairs were ligated, then released within the Visium CytAssist instrument for capture on the FFPE Visium Spatial Gene Expression Slide (10x Genomics, Product No. 1000520; User Guide, CG000495 Rev C). Spatial transcriptome sequencing libraries were prepared with 10x Genomics Visium, spatial gene expression slides & reagent kit accoring to manufacturer's construction