16S rRNA amplicon sequencing of Mus musculus: wild-type male feces, non-cohoused, 8 weeks, replicate 4
Fecal samples were collected from 8-week-old mice maintained under cohoused and non-cohoused conditions to assess the impact of host genotype and microbial transfer on gut microbiota composition. Samples were immediately frozen and stored at 80 C. Microbial DNA was extracted using the PureLink Microbiome DNA Purification Kit, and the 16S rRNA gene was sequenced using paired-end reads on an Illumina NovaSeq 6000 platform.