GSM9325936: 17-CL1_shTENT5AB_1; Mus musculus; RNA-Seq
Total RNA was isolated with the RNeasy Mini Kit (Cat. No. 74104, Qiagen). Poly(A) containing mRNAs were enriched with Oligo(dT)25 Dynabead™ (61002, Thermo Fisher Scientific) from 5 μg of total RNA. Poly(A) mRNA from each shTENT5A/B knockdown cell line was used for library preparation.  Libraries were prepared using the Direct RNA Sequencing Kit (SQK-RNA002, Oxford Nanopore Technologies). Libraries were loaded to Nanopore R9 Flow Cells (FLO-MIN106D, Oxford Nanopore Technologies), and run on a MinION sequencer.