GSM8901200: female-KO-Tau.314; Mus musculus; RNA-Seq
Animals were transcardially perfused with 1× PBS followed by 4% paraformaldehyde in PBS. Following dissection, total RNA was extracted using Trizol-chloroform from 1 mm-thick slices of dHPC (comprising the CA1, CA2, CA3, and dentate gyrus). Total RNA quality was assessed on the Bioanalyzer platform using the RNA 6000 Nano assay (Agilent). mRNA was isolated from 300 ng total RNA using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (E7490L), and libraries were prepared using the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (E7770).