GSM9326139: hPA, Donor 2, perturb-seq, CRISPR; Homo sapiens; RNA-Seq
Paired screen gRNA library was delivered at MOI = 0.3 alongside gRNA-hit (MOI = 3) with Thy1.1 in place of the puromycin resistance cassette. Ten days after transduction, cells were collected for processing. Cells were collected and gRNA and gene expression libraries were prepared using the 10X High-throughput kit with 5' gRNA Direct Capture (10x Genomics) according to manufacturer protocol and sequenced on an Illumina Novaseq.