GSM8641197: FID-D3 trt3; Mus musculus; RNA-Seq
total RNA was isolated using Trizol Reagent (15596026, Thermo Fisher) according to the manufacturer's protocol. RNA from primary FAPs was extracted with Qiagen RNeasy Micro Kit (74004, Qiagen) following manufacturer's protocol. RNA-seq libraries were constructed at the ICBR Gene Expression and Genotyping Core Lab using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, United States) following the manufacturer's recommendations. A total of 1000 ng samples of RNA with RNA integrity numbers (RIN) greater than 5.6 were used