GSM8973648: hNPC(AG09429), PBS treatment, 24h, rep1; Homo sapiens; RNA-Seq
To extract RNA, cells were plated on 10cml dishes, washed with PBS, and then 2.5ml of TrypLE™ Express Enzyme (Thermo Fisher, Cat. No. 12604013) was added directly to the cells. Incubate the plates in humidified atmosphere with 5 % CO2 at 37℃ for 5 min. After incubation complete hNPC medium and pipet gently to detached the cells to  collect. To isolate RNA, we used a Direct-zol RNA miniprep kit (Zymo Research Corporation, Cat. No. R2052), following manufacturer's instructions including the optional DNAse step.For RNA sequencing, ten biological replicates were sequenced. Total RNA was enriched for polyA and sequenced 2x150 bp on a HiSeq machine.