Species chimeric nAChR receptors to assess neonicotinoid toxicity in a Drosophila model
Whole-genome sequencing of Drosophila melanogaster mutant generated using CRISPR/Cas9 genome editing to investigate the role of nicotinic acetylcholine receptor (nAChR) subunit composition in insecticide susceptibility. The chimeric mutant flies have the 2 ligand-binding domain replaced with that of the honey bee nAChR8 subunit. Genomic DNA was extracted from adult flies and sequenced using Illumina NovaSeq 6000 with paired-end reads to assess potential off-target effects and confirm successful editing.