GSM8729216: mod[07570]_L8_Input; Drosophila melanogaster; ncRNA-Seq
Ovary lystaes from repsective genotypes were incubated with Panx antibody overnight. To extract small RNAs bound to Panx, the beads, after the washing step, were treated with TRIzol, and the total RNA was extracted and submitted for sequencing. Prior to library preparation and sequencing, the RNA sample was supplemented with 1 µl of 10 µM 2S Block oligo (5'- TAC AAC CCT CAA CCA TAT GTA GTC CAA GCA/3SpC3/-3') to inhibit amplification of ribosomal RNA. Libraries were prepared by Novogene using the NEBNext Small RNA Library Prep Set for Illumina