GSM8697992: RNA_seq_MGUS_CB31; Homo sapiens; RNA-Seq
Plasma cells from bone marrow were isolated using the Miltenyi CD138 Multiple Myeloma MACS kit, following the manufacturer's standard protocol. Total RNA was extracted from an equal number of viable cells using QIAzol Lysis Reagent (Qiagen) and purified from DNA contamination through a digestion step with the RNase-Free DNase Set (Qiagen). RNA libraries were generated using an equal amount of RNA from each sample, following the protocol of the Illumina Stranded Total RNA Prep kit, with an initial ribosomial depletion step using Ribo-Zero Plus from Illumina.