RNA-seq of human brain endothelial cells treated with Nrf2 activator RTA-404
RNA-seq of human brain endothelial cells treated with Nrf2 activator RTA-404
Human Brain Microvascular Endothelia Cells (BECs) were purchase from Innoprot (P10361-IM, Elexalde Derio, Spain). All plates and dishes for endothelial cells culture were coated with 0.2% Gelatine (G9391, Sigma-Aldrich) and 10ug/mL fibronectin (F1141-5MG, Sigma-Aldrich) for 30 min at 37 °C. Cells were cultured in Endothelial Cell Growth Medium with premixed supplements (C22022, Promocell) and 1% antibiotic-antimycotic solution (11570486, Gibco). RNA extraction was carried out using the RNeasy Plus Micro Kit (Qiagen) as per the manufacturer's instructions. Libraries were prepared by Edinburgh Genomics using the Illumina TruSeq stranded mRNA-seq kit, according to the manufacturer's protocol (Illumina).