Heat stress response in the regenerative flatworm Macrostomum lignano
Heat stress response in the regenerative flatworm Macrostomum lignano
Animals were starved for 48 h, changing the sea water medium after first 24 h and right before the experiment.  Total RNA was extracted using TRIzolâ„¢ Reagent (Invitrogen). RNA-seq libraries were prepared using Smart-3SEQ protocol from 100 ng of total input RNA.