WT_2_E100032158L1C001 (CRX796927; CRA012701)
Total RNAs of terminal ileum tissue with PPs were extracted from samples using TRIzol (15596018, Invitrogen) and DNA digestion was carried out by DNaseI  (EN0521, Thermo Scientific). RNA quality was determined by examining A260/A280 with NanodropTMOneC spectrophotometer (ND-ONEC-W, Thermo Scientific). RNA Integrity was confirmed by 1.5% agarose gel electrophoresis. Qualified RNAs were finally quantified by QubitTM3 fluorometer (Q33216, Invitrogen) with QubitTM RNA HS Assay kit (Q32855, Invitrogen). About 2microg total RNA of each sample was used for BCR sequencing library preparation using KC-DigitalTM Stranded BCR-seq Library Prep Kit for Illumina 150 (DT0815-02, Seqhealth Technology) following the manufacturer's instruction. The kit eliminates duplication bias in PCR and sequencing steps, by using unique molecular identifier (UMI) of 8 random bases to label the pre-amplified cDNA molecules. submitted_library_strategy: BCR-Seq