GSM9474277: HFD-SM2; Mus musculus; ATAC-seq
ARM/SMs that were purified via FACS sorting were first washed with PBS and then lysed using a lysis buffer composed of 1 M Tris·Cl (pH 7.4), 5 M NaCl, 1 M MgCl2, and 10% Nonidet P-40 in nuclease-free H2O, with gentle agitation. Following this, the lysed samples were treated with Tn5 transposase. DNA was purified (Qiagen Min Elute Reaction Cleanup Kit) and amplified with 1X NEB Next High-Fidelity PCR Master Mix (New England Biolabs, MA) using barcoded primers. DNA library was purified with Min Elute PCR Purification Kit (Qiagen).