TP53 I195F mut, brain metastasis of ovarian cancer
RNA was isolated from biopsy samples (~25 mg tissue) using the RNeasy Mini kit (Qiagen), according to the manufacturer's directions. Libraries were prepared for RNA-Seq analysis using the SMART-Seq v4 Ultra low Input RNA kit (Takara), followed by addition of Illumina adapters and 150 bp paired end (PE150) sequencing on the Illumina NovaSeq 6000 platform (MedGenome Inc.). The paired output fasta files for each sample were checked for quality using FastQC, and adapters trimmed using FastqMcf and Cutadapt.