MN_3_Soil bacterial 16S sample
Bacterial 16S rRNA gene amplicon sequencing was performed on the Illumina NovaSeq 6000 platform. The V3-V4 hypervariable region was targeted for PCR amplification using universal primers: 338F (5'-ACTCCTACGGGAGGCAGCA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3'). A total of four treatment groups (CK, LN, MN, HN) were included, with three experimental replicates set for each group.