RNAseq of C. elegans: whole organism
rRNA was depleted using the RiboZero Plus kit (Illumina) to enrich mRNA. Paired-end, indexed, strand-specific RNA libraries were prepared using the Ultra II Directional kit (NEB). Libraries were sequenced on a NovaSeq (Illumina) to produce 2x150 bp reads. Approximately 50-70 million reads were produced per sample.