RNA-Seq data of 35S::PGA37-GR seedlings treated with CHX (CRX1168228; CRA018539)
mRNA was isolated with Oligo(dT) magnetic beads, and then broken into short fragments (about 200 bp). Taking these short fragments as templates, first-strand cDNA and second-strand cDNA were synthesized. The double strand cDNA was purified with a QiaQuick PCR extraction kit (QIAGEN, Cat. No.28106) and washed with EB buffer for end repair and single nucleotide A (adenine) addition. Next, the short fragments were ligated to Illumina sequencing adaptors. The fragments of a selected size were purified by agarose gel electrophoresis and enriched by PCR amplification.