72h
Extractions were performed using ZymoBIOMICS DNA Miniprep Kit (R2002, Zymo Research, Irvine, CA, USA). DNA ualities were estimated using an Agilent 4150 TapeStation (Agilent Technologies, Santa Clara, CA, USA). The pooled DNA from each sample was subsequently used to construct a sequencing library using the NEBNext Ultra II Library Prep Kit (New England Biolabs, Ipswich, MA, USA). Paired-end metagenome sequencing was performed on a NextSeq 550 sequencer (Illumina, San Diego, CA, USA) using the NextSeq High Output Kit v2 Sequencing Reagent Kit.