GSM8641121: Brain, exosome treated HFD, biological replicate 3; Mus musculus; RNA-Seq
Total RNA was extracted from E0771 cells treated with HFD or LFD-derived exosomes using the RNeasy Plus Mini Kit (QIAGEN, Cat. No. 74136), following the manufacturer's protocol. RNA integrity was assessed using an Agilent 2100 Bioanalyzer. Libraries were constructed using the Illumina TruSeq Stranded mRNA Library Prep Kit, as per the manufacturer's protocol. Final libraries were checked for quality using an Agilent Bioanalyzer and quantified using a Qubit fluorometer.