Whole genome of Blautia producta 1009924
Take 100 ng whole gene gDNA, randomly break it into fragments less than 500bp by ultrasonic fragmentation device (Covaris S220), then perform End repair by End Prep Enzyme Mix (including 5 'terminal phosphorylation and 3' terminal addition of 'A'), add sequencing splices at both ends, and purify the target fragment using magnetic beads. Finally, the amplification was performed with P5 and P7 primers. Library quality was measured using Agilent 2100 bioanalyzer (Agilent Technologies,Palo Alto,CA,USA), library concentration was measured by Qubit 3.0, and qualified libraries were cluster prepared and sequenced.