hPADI4 covalent RaPID selection 1h inc Round 1
DNA libraries, including 6-12 degenerate NNK codons in a ratio 0.001 NNKn=6:0.032 NNKn=7:1 NNKn=8:32 NNKn=9:33 NNKn=10:33 NNKn=11:11 NNKn=12 (5-TAATACGACTCACTATAGGGTTAACTTTAAGAAGGAGATATACATATG(NNK)nTGCGGCAGCGGCAGCGGCAGCTAGGACGGGGGGCGGAAA) were subjected to 5 rounds of RaPID selection using hPADI4 as protein target. Double indexed libraries (Nextera XT indices) were prepared with the DNA recovered after each round and sequenced on a MiSeq platform (Illumina) using a v3 chip as single 151 cycle reads.