GSM9069579: BHLHE40 knockdown, mRNA-derived cDNA; Homo sapiens; RNA-Seq
Total RNA was extracted from cells using the RNeasy Micro kit (QIAGEN N.V.) according to the enclosed instructions. A full-length cDNA was prepared using a SMART-Seq HT Kit (Takara Bio), following the instructions provided with the kit. Then, a NexteraXT DNA Library Preparation Kit (Illumina) was used to prepare the cDNA library.