MOA-seq HUVEC 0hr
DNA quality was assessed by spectrophotometry, and digestion patterns were visualized using gel electrophoresis on a 1% agarose, 1X TBE gel. DNA was further quality-controlled using an Agilent 2100 Bioanalyzer, Q-PCR, and a Qubit 2.0 Fluorometer. Size-selected, indexed libraries were prepared from purified DNA and sequenced using paired-end 150 bp reads on the Illumina HiSeq X Ten platform. Two biological replicates were analysed per condition.