WES of Mouse# 167 Lobe 2 Tumor 4 KRASG12D TP53 and RAD18 Knockout Lung Adenocarcinoma
Mouse lung tumors were induced by expression of mutant KRAS in TP53 knockout and RAD18 knockout background. Tumors were collected and DNA was extracted from using a kit according to manufacturers protocol (Qaigen, 69506). DNA sample integrity and purity were assessed using Agilent 5400 instument. After genomic DNA fragmentation, adaptors were added then specific primers were using to PCR-amplify exons out of each sample. Reverse PCR primers were then used to capture the the amplified regions. The prepared library was sequenced on Illumina NovaSeq 6000 PE150 platform.