GSM9326118: shCtrl replicate 1; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol reagent according to the manufacturer's instructions. RNA concentration and purity were measured using a Nanodrop spectrophotometer. Additional RNA quality control for sequencing was performed by DarwinBio. PolyA-enriched RNA-seq libraries were prepared using an Illumina-compatible library preparation kit according to the manufacturer's protocol. Libraries were quantified, pooled, and sequenced on an Illumina NovaSeq platform (paired-end 150 bp).