RNA-Seq of hiphop[mel],HOAP[mel] biol rep 3: Drosophila melanogaster adult ovaries
We dissected ovaries into RNAlater Stabilization Solution (Thermo Fisher Scientific, Waltham, MA) and prepared total RNA using the MirVana miRNA isolation kit (Thermo Fisher Scientific, Waltham, MA). We used TURBO DNase (Thermo Fisher Scientific, Waltham, MA) to remove DNA contaminants. For total RNA sequencing, Admera Health (South Plainfield, NJ) depleted rRNA using the QIAseq FastSelect rRNA Fly kit (QIAGEN, Hilden, Germany) and then prepared libraries using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, Ipswich, MA).  Admera Health then performed next-generation sequencing on libraries using Illumina 2x150 for a total of 60M paired-end reads per sample.