Study of the heterogeneity of mammalian pluripotent stem cell lines (monkey ESC nhsmv)
Study of the heterogeneity of mammalian pluripotent stem cell lines (monkey ESC nhsmv)
Cells were trypsinized and inactivation was done with culture medium. Cells were then passed through a cell strainer and centrifuged. Cells were seeded onto gelatin coated dishes for 20 minutes at 37 degrees to allow feeders to attach to the plate. Supernatant was collected and cell pellet was washed twice before passing through a 35 um cell strainer. Final cell viability and counting was done with an automatic cell counter. Chromium Singe Cell 3' v3, 10x Genomics Chromium Singe Cell 3' v3, 10x Genomics