GSM9476316: RH30 ATAC-Seq; Homo sapiens; ATAC-seq
For ATAC sequencing, we follow the protocol established by Buenrostro et al. (Curr. Protoc. Mol. Biol., 2015). Briefly, around 50,000 cells were used for nuclei preparation. Chromatin tagmentation and library construction were carried out using the Illumina@ Tagment DNA Enzyme and Buffer kit (Illumina). ATACseq DNA libraries were sequenced on the Illumina NextSeq 2000.