RNA-seq of Aggregatibacter actinomycetemcomitans 624 grown on TSAYE plates replicate 4
Incubate the autoclaved Tryptic Soy Agar with Yeast Extract (TSAYE) plates at 37C in an oxic environment with 5% CO2. Regularly monitor the plates for the growth of colony biofilms and harvest at earlier time points with bacterial growth