p_lpp_rgn_rnai3
The total RNA was isolated from fat bodies of adult flies with P. entomophilaP.e oral infection and control flies (fed with 5% sucrose solution) at 18hpi. Perform three biological replicates of the fly infection. The sequencing was performed using the Illumina NovaSeq 6000 platform at Beijing Novogene Bioinformatics Technology Co., Ltd. Skewer (V0.2.2) was utilized for quality trimming and adapter trimming of the reads. The filtered reads were then aligned to the Drosophila genome using STAR version 2.7.4. Gene expression profiles were generated using StringTie (V2.1.2), and each gene's expression was normalized using DESeq2 (V1.20). Following normalization, a differential expression analysis was conducted.