GSM8800062: Patient6_MicrocysticTissue6C; Homo sapiens; RNA-Seq
Following RNA extraction using the RNeasy Plus Mini kit (Qiagen) according to the manufacturer's protocol, RNA concentration and quality were determined using DropSense96 (Perkin Elemer) and Bioanalyzer (Agilent). For RNA-Seq library preparation, the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina (Bioké) was used according to the manufacturer's protocol. After quality assessment of the obtained libraries, an equimolar pool was prepared. A qPCR was then carried out using the Kapa SYBR FAST Universal qPCR kit for Illumina (Roche).