hic data of Cheilodactylus zonatus
Muscle tissue from the foot of G. zonatus was used to construct the Hi-C library according to a standard protocol with several modifications. Briefly, the tissue was ground in liquid nitrogen and cross-linked with a 4% formaldehyde solution to preserve the 3D structure of the DNA. After overnight digestion with the 4-cutter restriction enzyme MboI, the DNA ends were labeled with biotin-14-dCTP. The subsequent steps involved blunt-end ligation of the cross-linked fragments, re-ligation of the proximal chromatin DNA, and reverse crosslinking of the nuclear complexes. DNA was then purified using phenol-chloroform extraction. Following the removal of biotin from non-ligated fragment ends and the repair of sheared fragment ends, biotin-labeled Hi-C samples were enriched, ligated with sequencing adapters, and amplified by polymerase chain reaction (PCR, 1214 cycles). The qualified library was sequenced on the Illumina Hiseq platform. After adapter removal and filtering of low-quality reads using Trim Galore (v0.6.10, https://github.com/FelixKrueger/TrimGalore)