16S rRNA gene sequencing of human skin microbiome: cheek
The V1-V3 region of the 16S rRNA gene was amplified using NEB HiFidelity DNA polymerase (New England Biolabs) with the 28F (5 GAGTTTGATCNTGGCTCAG) and 519R primers (5 GTNTTACNGCGGCKGCTG). Barcoding was performed using Nextera XT dual index primers (Illumina) and NEB HiFidelity DNA polymerase (New England Biolabs). Libraries were sequenced on an Illumina MiSeq 2x300 flow cell.