Ministeria vibrans ATCC 50519 xenic culture
Metagenomic DNA was extracted using the Invitrogen™ PureLink™ Microbiome DNA Purification Kit (ThermoFisher). 16S rDNA amplicon sequencing was then performed by the Molecular Research LP company (USA) using bTEFAP® illumina MiSeq with the V4 variable region amplified using PCR primers 515F (Caporaso; GTGCCAGCMGCCGCGGTAA) and 806R (Caporaso; GGACTACHVGGGTWTCTAAT).