GSM8768478: Input_2; Canis lupus familiaris; RIP-Seq
Total RNAs were extracted using TRIzol Reagent (Invitrogen, cat. NO 15596026）. DNA digestion was carried out after RNA extraction by DNaseI. Anti-ac4C antibodies was used to pull down modified RNA . RNA libraries for RNA-seq were prepared using components of the KCTM Digital mRNA Library Prep Kit (Seqhealth Tech. Co., Ltd., Wuhan,China)