GSM8973266: SH121_CH; Homo sapiens; RNA-Seq
ECs were collected in RLT + ß-mercaptoethanol and RNA isolated with the RNeasy Micro kit (Qiagen) with on-column DNAseI treatment. RNA purity and quality was analyzed with Bioanalyzer and only samples with a RNA Integrity Number above 8 were used for subsequent steps. Libraries were synthesized with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) starting with 20-50 ug total RNA