snRNAseq-mouse placenta-IVF
Mouse placentas were generated by transferring E3.5 blastocysts conceived either in vivo (FB) or by in vitro fertilization (IVF) into pseudopregnant recipients. On embryonic day 12.5, placentas were collected, flash-frozen, and processed for single-nucleus RNA sequencing. Nuclei were isolated with the Singulator 100, counted, and barcoded using the 10X Genomics Chromium Next GEM Single Cell 3 v3.1 kit. Libraries were sequenced on an Illumina NovaSeq 6000. Data processing included quality control, clustering, and differential gene expression analysis to compare IVF and FB placentas.