S-4d-2 (CRX1089521; CRA016935)
Midgut RNA was harvested using the Arcturus PicoPure RNA Isolation Kit (Applied Biosystems). RNA was then amplified using the Arcturus RiboAmp HS PLUS RNA Amplification Kit (Applied Biosystems). RNA libraries for RNA-seq were prepared using the NEB Next Ultra RNA Library Prep Kit according to standard protocols.