Raw PacBio reads of Hymenolepis diminuta
DNA was sheared to an appropriate size range (10-20 kb) using Megaruptor 3 (Diagenode) followed by SMRTbell cleanupbeads. The sequencing library was constructed following manufacturers protocols using SMRTbell Prep kit 3.0. The final library was size selected on a Pippin HT (Sage Science) using S1 marker with a 10-25 kb size selection. The recovered final library was quantified with Qubit HS kit (Invitrogen) and size checked on Femto Pulse System (Agilent). The final library was prepared for sequencing with PacBio Sequel II Sequencing kit 3.1for HiFi library, loaded on Revio SMRT cells, and sequenced in CCS mode for 24 hours.