RNA-seq of pestalotiopsis trachicarpciola cultured on PDB medium
Mycelial plugs (6mm diameter) from the edge of 3-day-old colony of the strain GZSQ-4 was transferred to PDA-matcha mixed medium and inoculated in a incubator at 25C for 7 d. Mycelia were ground into fine powder under the protection of liquid nitrogen. About 100 mg mycelia powder were used for RNA extraction. Total RNAs were extracted using the EASYspin Plus Plant RNA Kit (Aidlab, China). A total amount of 4 g RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.