GSM9219834: Susceptible,Panicle,high night temperature,mock inoculated,rep 3; Oryza sativa; RNA-Seq
Panicles were ground using 1600 MiniG®️ automated tissue homogenizer (Spex Sample Prep, Metuchen NJ), and total RNA was extracted using Trizol reagent (Life Technologies, Carlsbad, CA). RNA was precipitated with 5M Potassium Acetate pH 5.2, and 100% ice-cold ethanol and air-dried. RNA pellets were resuspended in 20 µl of RNAse-free water RNA purity and integrity was evaluated using the ExperionTM Automated Electrophoresis system (BioRad, Hercules, CA).  RNA sequencing was performed by Novogene (Davis, CA) for 150 bp paired-end read sequencing on the Illumina PE250 platform