GSM9712342: Murine intracranial vascular endothelial cells, Sham_Cells_Set1; Mus musculus; RNA-Seq
CD31⁺CD11b⁻CD45⁻ brain endothelial cells were FACS-sorted directly into 96-well lysis buffer (Bio-Rad) supplemented with RNase inhibitor, oligo(dT), dNTPs, and ERCC spike-in controls. Sorted samples were stored at −80 °C until use. Sequencing libraries were generated with the Illumina Nextera XT kit and pooled for sequencing on the NovaSeq 6000 platform. Sequencing was performed in 2 × 100 bp paired-end mode with dual 8 bp indexes (200-cycle kit). On average, 7.5 million paired-end reads were obtained per single cell.