16S-Seq of mouse gut metagenome: anaerobic droplet microfluidic cultivation day 17
DNA was extracted using DNeasy Ultraclean Microbial Kit (Qiagen), Q5 polymerase along with primers 314F (CCT ACG GGN GGC WGC AG) and 758R (GAC TAC HVG GGT ATC TAA TCC) were used for amplification of the extracted DNA