GSM9219086: MDAMB231_gCTRL_0nM_0h_rep1; Homo sapiens; RNA-Seq
After PBS wash twice, cells were directly lysed on 10-cm dish with Buffer RLT. Lysate was collected and total RNA was extracted using the RNeasy Mini kit (Qiagen) according to the manufacturer's protocol. RNA-seq libraries were generated using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) according to the manufacturer's instructions.