GSM9116250: NCI-H526 VC 5wk tumor 3; Homo sapiens; RNA-Seq
Total RNA from homogenized tumors was isolated using RNeasy Mini Kit (Qiagen). After quality check with Agilent BioAnalyzer and Qubit, 1 µg RNA per sample was used for poly(A) mRNA isolation. cDNA libraries were prepared using NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina, following manufacturer instructions. Barcode-indexed libraries were purified with AMPure XP beads (Beckman Coulter) and checked for library quality using Agilent BioAnalyzer DNA Chip and quantified using KAPA qPCR.