RNA-Seq of Petunia: pRNAi-GG-PhCTL1
The flower buds < 1 cm were harvested for RNA-seq analysis. Total RNA of flower buds samples from phctl1T0and the WT plants with three biological replicates were isolated using the TRIzol reagent (TransGen Biotech Co.), respectively, according to the manufacturers protocol. The integrity of the RNA extracted from the leaves was initially assessed using a 1% agarose gel. The quality, purity and concentration of the total RNA were evaluated through the utilisation of an Agilent 2100 and a NanoDrop spectrophotometer. The RNA samples were dispatched to Beijing Biomarker Technologies Co., LTD., for transcriptome sequencing, which was conducted using the BGISEQ-500 platform.