RNA-Seq of Perkinsus chesapeaki: trophozoite
Cells were routinely passaged every 3-4 days by transferring 200-400 l of stationary-phase culture into T-25 culture flasks containing 10 ml of medium. Two days post-inoculation, cells were harvested using TRIzol. RNA extraction was conducted on the stored pellets using the Direct-zol RNA Purification Kit according to the manufacturer's guidelines. Subsequently, the extracted RNA was utilized for the creation of strand-specific RNA libraries using the TruSeq Stranded mRNA Sample Prep Kit. These libraries were then sequenced on the Illumina Novaseq 6000