ddRAD-Seq of Anolis gundlachi: adult male
Genomic DNA was isolated from lizard tail samples using Qiagens DNEasy Blood & Tissue Kit, following the manufacturer's purification of total DNA from animal tissues protocol. Extracted DNA quality was assessed using Qubit and Bioanalyzer instruments. Double digest restriction-site associated DNA sequencing libraries were constructed at the Sequencing and Genomics Facility of the University of Puerto Rico. Two restriction enzymes (PstI and MseI) were used to digest the DNA before ligating P1 sequencing adaptors with six-base barcode identifiers. 74 libraries were pooled into two sequencing runs with distinct samples and each library pool was sequenced on an Illumina NexSeq500 sequencer to obtain an average of 15.8 million paired-end 150bp reads per sample.