Nanopore sequencing reads of Gracilimonas pinellae BCB1
Colony was grown in BD Difco Marine Broth 2216 at room temperature in the dark. Upon visible growth, the culture was centrifuged at 1,700 RCF for 5 minutes. DNA was extracted from the pellet using Qiagens AllPrep DNA/RNA Mini kit and purified using Zymo Researchs Genomic DNA Clean & Concentrator-10. The purified DNA was quantified using the Qubit HS assay and the Nanodrop ND-1000 spectrophotometer, then visualized on a 1% (wt/vol) agarose gel before submission to Eurofins Genomics for Nanopore sequencing using the v14 library preparation chemistry, R10.4.1 flow cell and dna_r10.4.1_e8.2_400bps_hac@v4.2.0 basecalling model.