GSM8641245: scRNAseq FAPs & MuSC coculture, KO 3; Mus musculus; RNA-Seq
Freshly isolated cells were diluted to obtained a stock concentration of 1000 cells/ul. Cells were loaded into a Chromium Next GEM Chip G to generate a Gel Beads-in-emulsion (GEMs). Single-cell RNA-seq libraries were prepared using the Chromium Single Cell 3' Library & Gel Bead Kit v3.1 . The final libraries were diluted to 3 nM and sequenced on an Illumina NovaSeq6000 using the following read length: 28bp for Read1, 8 bp for I7 Index, and 91 bp for Read2. 10x Single Cell Genomics