GSM9393797: Tom+ and Tom– sorted CD19+ B cells loaded on cartridge 2; Mus musculus; RNA-Seq
FACS sorted B cells were labelled with BD Mouse immune Single-cell Multiplexing kit. Next a total of 60000 and 50000 viable cells were loaded on 2 separate BD Rhapsody cartridges and loaded on the BD Rhapsody Express Instrument, resulting in gravity diriven distribution of single cells into microwells. Single cell bead capture, lysis and barcoding and cDNA synthesis were performed according to manufacturer's instructions. Targeted PCR amplification was performed according to manufacturer's instructions. The BD Rhapsody Immune Response Panel Mm was supplemented with custom primers adding up to 463 detectable transcripts. cDNA libraries were purifed with AMPure XP Reagent and quantified with Qubit Fluorometer (dsDNA HS Assay Kit). Final cDNA library  quality was assessed with Agilent 2100 Bioanalyzer (High Sensitivity DNA Kit).