GSM8697611: human high-grade ovarian carcinoma sample 3; Homo sapiens; RNA-Seq
Tumors were minced and dissociated using the human tumor dissociation kit (Miltenyi Biotec), depleted for erytrocytes and filtered. Cells were cryopreserved until scRNA-seq was performed. If applicable, dead cells were removed using the dead cellremoval kit (Miltenyi Biotec). Single cells were captured using the BD Rhapsody HT Express and the BD Rhapsody Scanner. cDNA synthesis and WTA library constructions was perfomed according to the manufacturer's instructions.