RNA-Seq of benthamiana infected with CLSV
Total RNA was extracted using the Qiagen Plant RNeasy kit, and the quality check was performed with the Agilent 2100 Bioanalyzer. All samples showed typical plant total RNA peaks and passed abm internal quality control. The samples were subjected to RiboZero plant rRNA depletion followed by fragmentation, first and second strand synthesis, adenylation of 3 ends, adapter ligation, DNA fragment enrichment, and real-time PCR quantification. Project was completed with one sequencing run. Bcl files were converted to FASTQ data immediately after the run. Over 80 million paired-end reads were collected (2x75 bp PE).