GSM8521911: Snord89_ASO_Rep_2; Homo sapiens; RNA-Seq
RNA was isolated 48 hours after transfection using TRIzol (Thermo). PolyA-selected RNA-seq libraries were prepared by Novogene and sequenced on the Illumina NovaSeq (150 bp PE) platform. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. polyA mRNA-seq