CZ032_root_RNA
Total RNA was extracted from the root, leaf, stem and tassel using the TRIzol reagent (Invitrogen, USA) following the standard protocol. For Illumina paired-end sequencing, mRNA was reverse-transcribed into cDNA, and five libraries with a 350 bp insert size were constructed using the TruSeq RNA Library Preparation Kit (Illumina, USA) according to the manufacturers instructions. Sequencing was performed on the NovaSeq 6000 Platform in PE150 mode.