GSM8733442: Pearl millet lateral roots replicate two; Cenchrus americanus; RNA-Seq
After fifteen days of growth, the plexiglass was carefully removed to avoid damaging the roots and samples were taken from different root types: the primary root tip (5 cm apex), the crown root tips (5 cm apex) and lateral roots on primary roots. Roots from three plants were pooled together to make one sample. Roots tissues from three replicates were stored at -80°C before RNA extraction using the RNAeasy Plant Mini Kit (QIAGEN). RNA sequencing was performed by the Novogene Company Limited (United-Kingdom) on an Illumina platform. Briefly, messenger RNA was purified and double strand cDNA was synthesized. Library construction involved end repair, A-tailing, adaptor ligation, size selection, amplification and purification as per the provider's protocols.