In vitro resistance development of Staphylococcus aureus: antimicrobial exposure and genomic characterization
DNA extraction was performed using the DNeasy UltraClean Microbial Kit (Illumina, Qiagen, Germany) according to the manufacturers protocol. Subsequently, sequencing libraries were prepared with the Illumina DNA Prep Kit (Nextera DNA Flex) (Illumina, San Diego, CA, USA), and pooled libraries were sequenced using an Illumina iSeq 100 sequencer.