16S rDNA sequencing of intestinal contents in aged male mus musculus
Intestinal content from 13-month-old C57BL/6J mice was collected and stored at -80 C. Total microbial DNA was extracted, and the V3V4 region of the bacterial 16S rRNA gene was amplified using primers 341F/805R and sequenced on an Illumina NovaSeq 6000 platform (PE250).