GSM8950371: GFP_AD_ACA; Mus musculus; RNA-Seq
Lung tumors were dissected from mice and flash frozen. Nuclei were isolated using a salt-based buffer and NP-40 with mincing on ice. Nuclei were washed, resuspended and counted prior to loading onto a 10x Chromium chip. Libraries were constructed using the 10x Chromium single cell 3' library kit according to manufacturer protocol.