GSM8951035: RHH4123; Homo sapiens; RNA-Seq
Samples for RNA-seq were 1 μg in 10 μl, as assessed by the Agilent Bioanalyzer for RNA integrity, prior to library preparation.For RNaseR treatment, total RNA was first subjected to large ribosomal RNAs removal using RiboMinus™ Transcriptome Isolation Kit (ThermoScientific) before treatment with RNase R (epicentre) at 3 U of RNase R per µg of RNA. Digested RNA was purified with standard RNA Phenol/Chloroform/Isoamylic alcohol extraction. Library preparation was performed using the Illumina Truseq kit according to manufacturer's protocol.