GSM9476057: Liver, WT, HFD, rep4; Mus musculus; RNA-Seq
Total RNA was extracted from liver samples collected from female WT and LKO mice fed chow or HFD for 12 weeks using TRIzol® (ThermoFisher). Samples were processed for quality control, mRNA library preparation with Poly A enrichment by NEBNext Ultra II RNA, and sequencing of 20 million NovaSeq Paired-End150 reads by Illumina (Novogene, Sacramento, CA). Fastq files were provided, and analysis was performed using a previously optimized workflow.