GSM8800308: FL_NUP98-NSD1/WT1ko_leukemic cells Input; Homo sapiens; ChIP-Seq
For each condition, approximately 1 × 10⁷ NUP98-NSD1/WT1ko leukemic cells were crosslinked, followed by ChIP using PRDM16 or H3K27ac antibodies. The immunoprecipitated DNA was then purified using SPRIselect beads, followed by library preparation, including end repair, adapter ligation, and PCR amplification. Quality control was performed to assess fragment size and concentration before the samples were subjected to next-generation sequencing. Library construction was performed using the NEBNext Ultra II DNA Library Prep Kit.