GSM9618376: PDO-296, Flex Pool 2 (E2, E2+ICI, E2+HSD17B7i); Homo sapiens; RNA-Seq
Organoids were dissociated to single cells, fixed, and processed according to the 10x Genomics Chromium Fixed RNA Profiling (Flex) protocol. Probe hybridization and ligation were performed per manufacturer instructions. Single-cell gene expression libraries were prepared using the 10x Genomics Chromium Fixed RNA Profiling (Flex) kit with probe set v1.1.0 (GRCh38-2024-A). Samples were multiplexed using probe barcode-based sample indexing. Pool 1 contained samples OS01-OS04 (probe barcodes BC001-BC004) and Pool 2 contained samples OS05-OS07 (probe barcodes BC005-BC007). Libraries were sequenced on an Illumina NovaSeq X Plus with 101 PE reads.