GSM9068667: si-Ctrl_1_ATAC; Homo sapiens; ATAC-seq
Briefly, 1 × 10⁶ cells were dissociated into single cell for use. Then cells were mixed with ConA Beads and incubated with primary antibody overnight at 4°C. After incubating with secondary antibody, and pA-Tn5 Transposon, DNA was tagmentated by Tagmentation buffer and extracted by VAHTS DNA Clean Beads (Vazyme). Cells transfected with si-CHAF1A or infected with sh-NAT10 lentiviruses were collected. 1 × 10⁶ cells per group were used to prepare sequencing libraries with ATAC-Seq Library Prep Kit for Illumina (TD711-01, Vazyme Biotech, Nanjing, China).