16S rRNA V4 amplicon sequencing of human gut metagenome from 23_MB_336
V4 region of 16S rRNA gene amplified with primers 515F (GTGYCAGCMGCCGCGGTAA) and 806R (GGACTACNVGGGTWTCTAAT) using KAPA HiFi HotStart ReadyMix, 25 cycles. Amplicons purified with AMPure XP beads, indexed with Nextera adapters.