GSM8900887: Mouse heart, 149, T497A, 5 weeks after sham surgery, 15 weeks old, rep3; Mus musculus; RNA-Seq
Total RNA was extracted from murine cardiac tissue using the RNeasy Mini Kit  (74104, Qiagen, Hilden, Germany) following the manufacturer's instructions. RNA integrity was assessed using Agilent 2200 Tapestation and RNA Screentape (5067-5576, Agilent Technologies). RNA-seq libraries were generated from total RNA using the KAPA  mRNA HyperPrep Kit (KK8581, Roche) according to the manufacturer's protocol.  Libraries were sequenced on an Illumina NextSeq500 platform using  single-end 75-cycle high-output mode.