Dovetail Omni-C sequencing data for Melursus ursinus: male sample KB16889
Omni-C libraries were prepared from the cell lines using the DovetailTM Omni-CTM Kit (Dovetail Genomics, Scotts Valley, CA) according to the manufacturers protocol with slight modifications. Briefly, chromatin was fixed in place in the nucleus. The fixed chromatin was digested in situ with DNase I. After digestion, the cells were lysed with sodium dodecyl sulfate (SDS), and chromatin fragments were bound to chromatin capture beads. Chromatin ends were repaired and ligated to a biotinylated bridge adapter followed by proximity ligation of adapter containing ends. After proximity ligation, crosslinks were reversed and the DNA was purified from proteins. Purified DNA was treated to remove biotin that was not internal to ligated fragments. An Illumina sequencing library was generated using an NEB Ultra II DNA Library Prep kit (New England Biolabs, Ipswich, MA) with an Illumina compatible y-adaptor. Biotin-containing fragments were then captured using streptavidin beads. The post capture product was split into two replicates prior to PCR enrichment to preserve library complexity with each replicate receiving unique dual indices. The library was sequenced on an Illumina NovaSeq 6000 platform (Illumina, San Diego CA) to generate approximately 100 million, paired-end 150 basepair (bp) read pairs per gigabase (Gb) of genome size.