GSM9617871: Right Basolateral amygdala SNL 3; Rattus norvegicus; RNA-Seq
At the chronic stage of neuropathic pain (4 weeks post-SNL or -sham surgery), rats (SNL, n = 6; sham, n = 6) were euthanized via decapitation. The brains were rapidly extracted and oxygenated in ice-cold ACSF that contained the following (in mM): 125.0 NaCl, 2.6 KCl, 2.5 NaH2PO4, 1.3 CaCl2, 0.9 MgCl2, 21.0 NaHCO3, and 3.5 glucose. Coronal brain slices (1,000 μM) containing the left and right CeA were prepared using a Vibratome (VT1200S, Leica Biosystems, Nussloch, Germany) as described previously (Hein et al., 2021; Presto & Neugebauer, 2022; Thompson et al., 2018). The left and right CeA and BLA were dissected from freshly harvested slices for bulk RNA sequencing analysis. RNA library preparation and sequencing were performed at the University of Texas at Austin Genomic Facility. Illumina Tag-Seq of polyA enriched total RNA sequencing was performed (single end, 100 bp).