RNA-seq of mus musculus: osteocytes from humanized female APOE4 humeri
RNA was extracted from the humeri of 15-month-old female humanized APOE4 knock-in mice using the miRNeasy Mini Kit (Qiagen). For osteocyte-enriched bone isolation, surrounding soft tissues, epiphyses, and bone marrow were removed (Dole et al., 2021). Poly(A)-selected mRNA libraries were prepared from 250 ng of total RNA using the Universal Plus mRNA-Seq with NuQuant kit (Tecan, Cat. No. 0520). Strand-specific paired-end sequencing was performed on an Illumina NovaSeq X Plus platform.