GSM9871105: HCT116, PABPC1&PABPC4 AID, PABP1212 expression; Homo sapiens; RNA-Seq
Total RNA was phenol chloroform extracted and 1μg total RNA was used as an input for sequencing preparation. Sequencing libraries were prepared according to manufacturer instructions (Oxford Nanopore direct RNA kit SQK-RNA004) with one specific adaptation. The first ligation set was modified to instead ligate a set of custom adapters that would allow for sample barcoding and demultiplexing.