GSM8640270: CDK9 CUT&Tag; Homo sapiens; OTHER
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a E220 focused-ultrasonicator (Covaris). The CUT & Tag libraries were constructed using HyperactiveTM In-Situ ChIP Library Prep Kit (TD901, Vazyme, China) and sequenced on an Illumina NovaSeq platform, and 150-bp paired-end reads were generated. Fastp v 0.20.0 was used to remove the adapter and low-quality reads.