ONT long-read sequencing of rhizosphere metagenome: ACT pot trial, river soil, planted, biofertiliser, R02
Shotgun metagenomic DNA libraries from soil and rhizosphere (rhizosheath) samples were extracted with the QIAGEN DNeasy PowerSoil Pro kit, prepared with the ONT Native Barcoding Kit 96 V14 (SQK-NBD114.96) using end-prep and ligation, pooled and sequenced on an ONT PromethION, then basecalled and demultiplexed with Dorado v1.1.1 in super-accurate (sup) mode and quality filtered (median Q>=15, length >=300bp).