GSM8950840: deltaStpA_RNASeq_biol_rep2; Escherichia coli; RNA-Seq
Total RNA was isolated using the Qiagen RNeasy mini RNA isolation kit according to the manufacturer's instructions. Ribosomal RNA was removed from 200 ng of the starting material using the NEBNext rRNA depletion kit (bacteria) and the libraries were prepared using NEBNext Ultra II Directional RNA-Seq library preparation kit.