MeRIP-seq of Oncorhynchus mykiss: gill tissue from T0
This study used m6A RNA immunoprecipitation sequencing (MeRIP-seq) to profile transcriptome-wide RNA methylation. MeRIP-seq libraries were prepared from gill tissues of rainbow trout (Oncorhynchus mykiss). Total RNA from T0 and T30 samples was fragmented and subjected to m6A immunoprecipitation using an anti-m6A antibody, with matched input controls prepared in parallel. Libraries were amplified by PCR and sequenced as paired-end reads on an Illumina platform.