RNA-seq of Subject-12: Pre-intervention stool
Genomic DNA from fecal sample was extracted using the QIAamp PowerFecal Pro DNA Kit (QIAGEN, Hilden, Germany) according to the manufacturers instructions. The V3V4 hypervariable regions of the bacterial 16S rRNA gene were amplified using primers 341F (CCTACGGGNGGCWGCAG) and 805R (GACTACHVGGGTATCTAATCC). Paired-end sequencing was performed on the Illumina MiSeq platform (Illumina, San Diego, CA, USA) by Bioengineering Lab. Co., Ltd. (Kanagawa, Japan).