GSM9393791: Day 10 time-stamped TCL-1 mouse 95, FACS sorted Tom– B cells; Mus musculus; RNA-Seq
FACS sorted B cells were lysed in RNAzol (Sigma-Aldrich) and total RNA was extracted with Direct-zol RNA MiniPrep Kit (Zymo Research) according to manufacturer's instructions. cDNA libraries were constructed wit SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara) and amplified with Nextera XT DNA library prep kit (Illumina). Final cDNA libraries were purified with AMPure XP Reagent, quantified with Qubit Fluorometer (dsDNA HS Assay Kit) and quality was assessed with Agilent 2100 Bioanalyzer (High Sensitivity DNA Kit).