GSM8641836: TSC_3_rep1; Homo sapiens; RNA-Seq
Cells were washed in PBS and lysed directly in the culture plate using RLT Plus Buffer (from the RNeasy Plus Mini Kit (QIAGEN, 74136)) with 1% 2-mercaptoethanol. Total RNA was extracted using the RNeasy Plus Mini Kit (QIAGEN, 74136) according to the manufacturer's protocol. Total RNA libraries were prepared using the mRNA Capture KAPA HyperPrep Kit (Roche, 0000162206), after which 150 bp paired-end sequencing was performed on an Illumina NovaSeq X Plus system.