GSM9395597: H16, mesaconic acid, biol rep 1; Cupriavidus necator H16; RNA-Seq
Total RNA was isolated using the Qiagen RNeasy Mini Kit, and residual DNA was removed using the TURBO DNA-free Kit (Thermo Fisher), following the respective manufacturer protocols. RNA integrity and concentration were determined using an Agilent 2100 Bioanalyzer. Prokaryotic RNA library preparation and sequencing were performed by Novogene (Cambridge, UK). For rRNA removal, the Illumina Ribo-Zero Plus rRNA Depletion Kit was employed.