PacBio HIFI Sequencing of Lycorma delicatula
High Molecular Weight (HMW) DNA was extracted from the head, thorax and appendages of the starved adult female L. delicatula using the Qiagen Midi Kit and quantified using a Quibit 2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA). The abdomen was avoided to reduce the potential for sequencing obligate endosymbionts. For long-read sequencing, a PacBio SMRTbell library (~20kb) was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol. The library was bound to polymerase using the Sequel II Binding Kit 2.0 (PacBio) before being sequenced on PacBio Sequel II 8M SMRT cells to generate 17,184,679 PacBio CCS reads totaling 279.3 Gb (Gigabases) of data.