GSM8734561: sh-Hmgb1-1; Mus musculus; Hi-C
Briefly, cells were cross-linked, lysed, and digested with the restriction enzyme MboI(R0147, NEB). Biotin was incorporated into the cohesive ends of DNA fragments prior to proximity ligation using T4 DNA ligase. After ligation, the DNA was purified and sheared into smaller fragments. Subsequent steps, including end repair, adenylation, and adapter ligation, were conducted using the NEBNext End Repair Kit (E6050, NEB). Biotin-labeled DNA fragments were captured using MyOne Streptavidin T1 beads (65602, Invitrogen). The Hi-C libraries were amplified using the KAPA HiFi Library Amplification Kit (KK2620, KAPA Biosystems), and size selection of the DNA fragments was performed using AMPure XP beads (A63880, Beckman Coulter).