GSM8729784: 4sUseq UL3 Rep2; Homo sapiens; OTHER
then RNA isolated using using TRIzol (Invitrogen), Dnase digested (NEB), the nascent RNAs biotinylated (Biotonium)and nascent RNA's captured on streptavidin magnetic beads (Promega). Libraries were generated using the Ribo-Zero Gold and the TruSeq Stranded Total RNA library kit according to manufacturer's instructions (Illumina) at Expression Analysis (Durham, NC).