GSM8973306: HUVECs_9d_control_2; Homo sapiens; RNA-Seq
RNA was isolated using the PeqGOLD Total RNA kit (VWR) and stored at -80°C The amount of total RNA was quantified using the Qubit 2.0 Fluorometric Quantitation assay (Thermo Fisher Scientific, Waltham, MA, USA) and the RNA integrity number (RIN) was determined using the 2100 Bioanalyzer High-Resolution Automated Electrophoresis instrument (Agilent, Santa Clara, CA, USA). RNA-seq libraries were prepared with the NEBNext® Poly(A) mRNA Magnetic Isolation Module E7490 and the NEBNext® Ultra™ II Directional RNA sample preparation kit E7760 (New England Biolabs, Inc., Ipswich, MA, USA). NGS library concentrations were quantified with the Qubit 2.0 assay and the size distribution was assessed using the 2100 Bioanalyzer instrument. Before sequencing, sample-specific NGS libraries were diluted and pooled in equimolar amounts. Expression profiling libraries were sequenced on a NovaSeq 6000 instrument (Illumina, San Diego, CA, USA) following a 100-base-pair, paired-end recipe.