GSM9085318: frail 24h D52; Mus musculus; RNA-Seq
~25 mg portion of powdered gastrocnemius was homogenized in 600 µL of Zymo Tri Reagent. Total RNA was isolated using a Zymo RNA Miniprep kit including an on-column DNase treatment. Libraries were prepared using the NEBNext UltraExpress RNA Library Prep Kit according to the manufacturer's protocol.