GSM9069570: Input WT limb bud ectoderm E10.5, rep2; Mus musculus; ChIP-Seq
Ectoderms were isolated with fine forceps from E10.5 forelimb buds of Sp8-FLAG homozygous embryos (for Sp8) and WT embryos (for Sp6) after digestion with 0.25% trypsin for 20 min. on ince. The ectoderms of 50 forelimb buds were used for each replicate. ChIPmentation protocol was based on (Schmidl et al., 2015)