GSM9325784: hPA, gRNA-hit, INPUT, rep 3; Homo sapiens; ChIP-Seq
Supernatant containing sheared chromatin was incubated at 65°C overnight to reverse DNA-protein crosslinks. ChIP DNA was purified with QIAquick PCR Purification kit (Qiagen). ChIP-seq libraries were constructed with a KAPA HyperPrep kit (Roche). ChIP DNA was quantified and amplified with 7 PCR cycles after KAPA adapter ligation. Libraries were pooled and sequenced with 1% PhiX spike-in on an Illumina NextSeq with a P4 XLEAP-SBS reagent kit.