GSM9696010: WT_ATB1071_biol rep 1; Mus musculus; RNA-Seq
QIAzol Lysis Reagent was added to the brain tissue containing microtubes. The tissues were homogenized using Tungsten Carbide Beads and TissueLyser. Phase separation was performed using chloroform and a buffer in Rneasy Mini Kit. RNA wash and elution were conducted with RNeasy Mini Kit according to the manufacturer's instructions. RNA libraries for RNA-seq were prepared with TruSeq Stranded Mrna Library Prep kit following the manufacturer's instructions