GSM8734897: K5Cre [JH4_S4]; Mus musculus; RNA-Seq
Total RNA was isolated from heat shock isolated mouse epidermis and purified  using QiaZOL lysis (Qiagen), followed by DNase I digestion, and column clean up (Zymo). cDNA libraries for Illumina sequencing were constructed via the NEBNext Ultra Directional RNA Library Prep Kit (NEB) and NEBNext Multiplex Oligos for Illumina (NEB) according to the manufacturer's protocol.