GSM9396540: DMSO-pH7.4#2; Homo sapiens; RNA-Seq
Total RNA of cells was extracted using RNeasy mini Kit (Qiagen, Cat#: 74106) according to the manufacturer's instructions and digested with DNase I (Qiagen, Cat#: 79254). RNA sequencing (RNA-seq) libraries were constructed using a NEBNext rRNA Depletion Kit (NEB, Cat#: E7405X) and a NEBNext ultra RNA Library Prep kit (NEB, Cat#: E7775L), then subjected to a 50 bp paired-end sequencing run on NextSeq 2000 system (Illumina), using Illumina's NextSeq 2000 P3 Reagents (#20040559) following the manufacturer's instructions.