Soil metagenome: stoichiometry study of field plots
Extractions were performed on 0.25 g soil using a DNeasy PowerSoil Pro Kit. DNA quality was assessed via  gel electrophoresis; seven of the 24 samples failed QC due to low DNA counts as determined by qPCR. To account for low concentrations, the libraries were generated using AGRFs Illumina DNA Prep (M) workflow with 9 ng DNA input and 12 cycles of PCR. Sequencing was completed on a NovaSeq X Plus system with a 10B flow cell and 300 cycle XLEAP-SBS chemistry. Image analysis was performed in real time by the NovaSeq Control Software (NCS) v1.0.1.7385 and Real Time Analysis (RTA) v4.0.5 after which the Illumina DRAGEN BCL Convert 07.021.645.4.0.3 pipeline was used to generate the sequence data.