Y_SCI5
Total RNA was extracted from tissue samples using TRIzol Reagent, and the quality was assessed using the 5300 Bioanalyzer (Agilent). Quantification of the extracted RNA was performed with the ND-2000 (NanoDrop Technologies). High-quality RNA samples (OD260/280 = 1.82.2, OD260/230  2.0, RIN  6.5, 28S:18S  1.0, > 1 g) were utilized for library construction. RNA purification, reverse transcription, library preparation, and sequencing were carried out at Shanghai MajorBio Bio-pharm Biotechnology Co. Ltd., following Illumina protocols. The RNA-seq library was prepared using 1 g of total RNA, involving mRNA isolation with poly-A selection, cDNA synthesis with random hexamer primers, and end-repair with 'A' base addition. The size-selected libraries (300 bp) were then PCR amplified and sequenced on a NovaSeq 6000 sequencer (2  150 bp).