GSM9219372: MCF10A (shSCR and shHIF1A), HTO multiplexed treated with ligand, gene expression; Homo sapiens; RNA-Seq
Culture medium was removed, and cells were rinsed twice with PBS. Cells were then incubated in 0.05% trypsin-EDTA for about 5 minutes until they detached from the plate surface. After detachment, cells were gently triturated and the enzyme was neutralized using PBS containing 10% FBS. Cells were collected by centrifugation at 300 x g for 5 minutes and resuspended in PBS. All samples were multiplexed and underwent hashtag oligo staining according to the vendor's recommendations (TotalSeq-A, BioLegend). The library was prepared using the Chromium Single Cell 3' GEM, Library & Gel Bead Kit v3 (10X Genomics) to capture both gene expression and antibody-derived features.