RBFOX2_sh1_SSC_1 (CRX825693; CRA012921)
Use Trizol to extract sample RNA. In order to ensure the purity of the RNA and remove contaminating DNA, add an appropriate amount of RNase-free DNaseI. Make 3 biological replicates for each processed sample. The samples were sent to Novogene for construction of a eukaryotic chain-specific library. The samples that passed the quality inspection were constructed and sequenced using the Novaseq 6000 sequencing platform.