GSM8698198: p300_iHAT_NT_rep3_NT_A486_3; Xenopus laevis; RNA-Seq
Total RNA was isolated using the RNeasy Mini Kit (QIAGEN, 74104) according to the manufacturer's instructions. To lyse the embryonic tissue, samples were vortexed on high speed for 10 min at 4°C. DNase digestion was performed according to the manufacturer's instructions, using RNase-free DNase (QIAGEN, 79254). Per sample, 400 ng total RNA of animal cap (ectoderm) tissue and 300 ng of endoderm donor tissue were used to isolate mRNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490).  Sequencing libraries were generated using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7760), following the manufacturer's instructions, using 12-13 PCR amplification cycles. Libraries were multiplexed and sequenced on a NextSeq 6000 platform.