RNA-Seq of sus scrofa
otal RNA samples were isolated with mirVana miRNA Isolation Kit (Thermo Fisher Scientific Inc.), quantified using Nanodrop, and qualified by agarose gel electrophoresis. The mRNA is enriched using oligo (dT) magnetic beads. RNA-seq libraries were prepared using the KAPA Stranded RNA-Seq Library Prep Kit (Illumina, San Diego, CA, USA), qualified with the Agilent 2100 Bioanalyzer, and quantified by the qPCR absolute quantification method. Sequencing was performed with Illumina NovaSeq 6000.