GSM8950760: Amiodarone modeling group,scRNAseq; Rattus norvegicus; RNA-Seq
Cell suspension samples were made up of equal numbers of cells selected from each group. Single cells were stained with Calcein AM and Draq7, and cell numbers and viability were determined with a BD Rhapsody™ scanner (BD Biosciences).The cells were loaded in one BD Rhapsody micro-well cartridge, followed by the cell capture beads in excess to ensure that nearly every micro-well contains one bead. The excess beads were washed off so that each microwell had a bead with unique molecular identifiers (UMI) and Barcode paired with a cell. After lysing the cells with lysis buffer, the cell capture beads were retrieved and washed prior to performing reverse transcription. scRNAseq