GSM9394171: S2-007_n1; Homo sapiens; RNA-Seq
Total RNA from S2-007, S2-020, and S2-028 cells were recovered using RNeasy Mini Kit according to the manufacturer's instructions. To purify the strand-specific mRNA library, the mRNA was reverse transcribed into cDNA using random primers, and then the synthesized double-strand cDNA was fragmented, and the cDNA was subsequently ligated adapters. Transcriptome sequencing in strand-specific mRNA-seq library was performed using illumine NovaSeq 6000 Sequencing System.