DNA sequencing 16S bumblebee queen
Ten fertilized queens of Bombus pauloensis were collected and transferred to the laboratory. They were caged and fed with two different diets ad libitum: monofloral pollen (Eucalyptus grandis) or polyfloral pollen. After the microcolonies were developed, at 5 days, queens and workers from both groups were sampled, guts were extracted, DNA was obtained and subjected to 16S rRNA gene sequencing to evaluate the gut microbiota. DNA was also subjected to PCR to evaluate the presence of Nosema ceranae, Nosema apis, Nosema bombi and Lotmaria passim.