16S rRNA gene sequencing of AGS_ Reactor 2
Amplification of the 16S rRNA gene was performed using primers 341F (CCTACGGGRSGCAGCAG)and 806R (GGACTACHVGGGTWTCTAAT) with sequencing conducted by Neoprospecta (Florianpolis, SC, Brazil). Sequencing was performed on the MiSeq platform (Illumina Inc., USA) with the 300-cycle, single-end V2 kit.