WGS of wild-type
Genomic DNA was isolated using a PureLink Genomic DNA Mini Kit (Invitrogen). A Qubit 2.0 spectrophotometer with the dsDNA Broad Range Quantitation kit (Invitrogen) was used to measure DNA concentrations. Illumina sequencing libraries were prepared using the tagmentation-based and PCR-based Illumina DNA Prep kit and custom IDT 10bp unique dual indices (UDI) with a target insert size of 320 bp.