Amplicon sequencing of food waste fermentation microbial community: 140 C hydrothermal pretreatment supernatant fermentation, Day 4,  replicate 3
Microbial DNA was extracted from the fermentation samples using a commercial DNA extraction kit. The V4 hypervariable region of the bacterial 16S rRNA gene was amplified using primers 515F and 806R. PCR amplicons were purified and quantified before library preparation. Amplicon libraries were constructed following the Illumina protocol and sequenced on the Illumina MiSeq platform using a paired-end 2250 bp strategy. Raw paired-end reads were merged using FLASH prior to submission, and only merged single-end reads are available for upload.