GSM9034913: Mx1-Elk1_MEP_replicate2; Mus musculus; RNA-Seq
For single cell RNAseq, CD3/CD19/NK1.1/Ter119－ CD11b+ and CD3/CD19/NK1.1/Ter119－C-kit+ cells derived from Mx1-Elk1 and littermate control mice are sorted and these two cell populations are mixed together at the ratio 1:2. For bulk-cell RNAseq,  LSK, CMP, GMP, MEP, immature neutrophils and mature neutrophils derived from  Mx1-Elk1 and littermate control mice were sorted and collected with PCR tube with 0.5%BSA-PBS buffer. 3000 cells of each population were used for each sample. For single cell RNA sequenceing, sorted cells are harvested by 10X Genomics Chromium system, then single cell libraries are generated by this system. RNA sequencing are perfomed by NovaSeq X plus system (library construction and sequencing of single cell were performed by HaploX company)..  For bulk-cell RNA sequence, all transcripts were converted into dsDNA for each population were generated according to previous reported procedures (Dong, Y., et al., Cells, 2019. 8(9), Tang, F., et al., Nat. Protoc. 2010, 5, 516–535.). Libraries were constructed by TruePrep DNA Library Prep Kit V2 for Illumina (TD503, Vazyme, Co.Ltd) and all the libraries were sequenced using the Illumina NovaSeq X plus system (HaploX company. Ltd.).