Illumina MiSeq paired-end Sequencing of KAS22022957 (KAE22248530)
Metagenome sequence analysis of jogi-jeot
Metagenomic DNA was extracted from the jeotgal using NucleoSpin kit for soil Macherey-Nagel, Duren, Germany. About 0.5 g jeotgal was used for DNA extraction following homogenization of the fish and liquid in jeotgal in a blender. For bacterial amplification, 341F and 805R primers were used, and i5 forward primer and i7 reverse primer were used for secondary amplification for attaching the Illumina NexTera barcode. After quality control, primers were trimmed with the Chunlab program cut-off of 0.8, and sequencing of the V3-V4 region from 16S rDNA was performed by Chunlab Inc. Seoul, Korea using the Illumina MiSeq platform. Bioinformatics analysis was performed using EzBioCloud program Chunlab Inc., which uses USEARCH to detect chimeras on reads that have <97% similarity.