RNA-Seq of Mytilus edulis gill: Family X3, Slow Grower, Triploid, 18C
Stranded paired-end 100 bp libraries were generated from 250 ng of total RNA with mRNA enrichment and library preparation performed using the Illumina Stranded mRNA Prep Kit (San Diego, CA, USA) following the manufacturers protocols. Libraries were quantified using the Universal KAPA Library Quantification Kit (Kapa Biosystems, Wilmington, MA, USA). Average fragment size was determined using a Fragment Analyzer 5300 (Agilent, Mississauga, ON, Canada) before the libraries were normalized, pooled, denatured in 0.02 N NaOH and then neutralized using pre-load buffer. The pool was loaded at 150 pM on an Illumina NovaSeq X Plus 25B lane following the manufacturers protocol. The run was performed for 2x100 cycles (paired-end mode).