Illumina NovaSeq 6000 paired-end Sequencing of KAS22024181 (KAE22250590)
Serum miRNA was extracted from cows naturally infected with Johne's disease. miRNA-seq was performed to identify the immune regulation mechanism.
Extraction of miRNA from bovine serum was performed by Qiagen serum miRNA extraction kit. Sequencing library was constructed with NEXTflex llumina Small RNA Sequencing kit v3.