GSM9115947: LIF25; Mus musculus; RNA-Seq
Total RNA was extracted using RiboPure RNA Purification (Ambion) kit, following the manufacturer's instructions. RNA concentration was quantified in BioTek Epoch (Agilent). RNA-seq libraries were made by an outsourced service (Indegene) with the NextSeq 500/550 High Output Kit v2.5 (Illumina). Two biological replicates were used per group (samples coming from different mice within the same group). Sequencing depth ranged between 40-45 million reads per sample and reads length is of 76bp