FR112N ETOH R1 male head RNA-seq
RNA was extracted from the heads of 30 male flies using the NucleoZol one phase RNA purification kit (Macherey-Nagel). Library preparation and sequencing was performed by BGI (Wuhan, China). The libraries were barcoded and pooled, and approximately two million reads were generated per library on the Illumina NextSeq. The data was demultiplexed prior to delivery.