MeRIP sequencing analyses of human liver cancer cell line HepG2
MeRIP sequencing analyses of human liver cancer cell line HepG2
HepG2 cells were cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum. The cells were maintained at 37 °C in a 5% CO2 humidified atmosphere. When the cell density reached approximately 80%, the cells were collected. 1x10^7 HepG2 cells were used in shCtrl and YTHDC1 knockdown group. Total RNA was isolated by Trizol (Invitrogen) according to the manufacturer's protocols. RNA was purified by NEBNext® rRNA Depletion Kit (NEB, USA). Fragmented and immunoprecipitated with anti-m6A antibody (Synaptic Systems, 202003). The RNA libraries were constructed using the NEBNext Ultra Directional RNA Library PrepKit (NEB, USA).