GSM9772176: Control; Drosophila melanogaster; RNA-Seq
100 female CantonS red flies were dissected in ice cold Schneider's medium. Optic lobes were removed and transferred to a tube with 3mg/ml of dispase. Samples were incubated in a thermoshaker for 15 min at 25C and 1000 rpm. To stop the enzymatic reaction, cells were rinsed three times with Schneider's with table top spins in between. Cells were resuspended in 60 ul of DPBS with 0.04% BSA. Cell suspensions were FACS sorted using an Aria Cell Sorter (using a 100 um nozzle and 1.25 drop precision) to ensure cell viability and adjust concentration. Cells were kept on ice until processed for 10x Genomics. Single Cell 3' Library and Gel Bead Kit following the manufacturer's recommendation protocol Single Cell 3' Library and Gel Bead Kit following the manufacturer's recommendation protocol and the libraries were sequenced on an Illumina NovaSeq 6000 in the Centre for Applied Genomics at the Children's Hospital of Philadelphia (CHOP).