GSM8608277: IFN-2; Homo sapiens; RNA-Seq
Total RNA was extracted as described above using the NucleoSpin RNA kit (Macherey Nagel, cat. no. 740955). The RNA integrity was monitored using Bioanalyzer analysis (Agilent, RIN: 9.7–10). About 500 pg of RNA per sample was reversetranscribed and amplified using a modified SMARTseq2 protocol (Rambow et al. 2018). Prior to generating sequencing libraries using the NexteraXT kit (Illumina, cat. no. FC-131-10), cDNA profiles were monitored using the Bioanalyzer. Sequencing was performed on a Nextseq500 platform