GSM9512478: 2225 Tumor, Trametinib, CP-3580; Mus musculus; RNA-Seq
Cryopreserved tumors were homogenized using a tissue homogenizer. The RNeasy Mini Kit (#74104, Qiagen) was used to isolate the RNA according to the manufacturer's protocol. The Agilent Bioanalyzer was used to asses the mRNA quality and libraries for mRNA-seq were constructed using total RNA and the Illumina TruSeq mRNA sample preparation kit.