H3K4me3-targeted CUT&Tag-Seq of homo sapiens: shCon organoid
Organoids were cultured for seven days and processed using the Hyperactive Universal CUT&Tag Assay Kit (Vazyme, TD903-01, China), with each epigenetic mark assayed in six biological replicates. After the CUT&Tag reaction, DNA purification, pre-amplification, library construction, and quality control (including assessments of tagmentation efficiency, DNA integrity, and library concentration) were performed. Sequencing was carried out on a DNBSEQ-T7 instrument (BGI, China) in PE150 mode, achieving approximately 6 Gb per sample.