GSM8774125: ME180 clone IX44; Homo sapiens; OTHER
Genomic DNA of selected clones was isolated using Pure Link genomic DNA Mini kit (Invitrogen, MA, United States), and quality and concentration were assessed using NanoDrop spectrophotometer Libraries were constructed with 100 ng of QC-passed gDNA using the Illumina TruSeq Exome Library Prep Kit, hybridized to exome oligo probes with unique barcoding, validated with Qubit and Bioanalyzer, and sequenced on an Illumina NovaSeq NGS System to generate paired-end 150 bp reads.