GSM8742960: PC3,Control siRNA R2; Homo sapiens; RNA-Seq
The immunoprecipitated and input DNA was purified using the MinElute PCR Purification Kit (#28006, Qiagen). Total RNA was isolated using the EZ-10 DNAaway RNA Mini-Preps Kit (#B618133, Sangon Biotech). ChIP-seq libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (#E7103L, NEB) according to the manufacturer's instructions. RNA-seq libraries were prepared using the Stranded mRNA-seq Lib Prep Module (RK20349, Abclonal).