RADseq of Erysimum graecum ACHR1
Total genomic DNA was extracted from silica gel-dried leaves using the Exgene Plant SV kit (GeneAll, Seoul, Korea) and purified with 0.4x AMPure XP beads (Beckman Coulter, Indianapolis, USA). The double-digest RADseq libraries were prepared according to the protocol of Peterson et al. (2012) with some modifications as described in detail in lenker et al. (2024). The libraries were sequenced with 150-bp paired-end reads on Illumina systems at Novogene, Cambridge, UK.