GSM9193470: MDAPCA2B, 1% ethanol (control), input rep2; Homo sapiens; ChIP-Seq
Cells were harvested in suspension and prepared for ChIP-seq according to the SimpleChIP® Enzymatic Chromatin IP Kit (Cell Signaling Technology #9003). 0.75 µl of micrococcal nuclease and 5 sonication cycles of 125 W, 20 khz, in 20 second intervals were used to digest chromatin. H3K27ac and IgG ChIP were performed in paired R1881-treated and ethanol-treated samples. ChIPs were run in duplicate. 5 µg H3K27ac (Active Motif 39133, RRID:AB_2561016), and 1 µg Rabbit IgG (Cell Signaling Technology #2729, RRID:AB_1031062) were used as ChIP antibodies. 20 ng samples were submitted to Novogene for DNA library preparation and sequencing with Illumina PE150 NovaSeq 6000.