GSM8645491: PXB Cells, PCSK9-EE, 1; Homo sapiens; OTHER
Genomic DNA was extracted from primary liver cells using simultaneous RNA and DNA extraction from cultured cells without splitting lysate protocol and reagents (Beckman Coulter). Genomic DNA (gDNA) quality and quantification assessment was done using Qubit fluorometric quantification and fragment analyzer to measure DNA fragment lengths. Sequencing library was prepared using NEBNext® Enzymatic Methyl-seq Kit with input gDNA (200 ng) was sheared using Bioruptor Pico to an average fragment size of ~300bp. Sequencing was performed on an Illumina NovaSeq6000 using 150bp paired-end reads, resulting in at least 600 million read pairs per library. Two sequencing runs were merged for analysis.