GSM9512796: C. scindens supplemented with riboflavin at 14 hours - rep A; [Clostridium] scindens; RNA-Seq
Total RNA was extracted using the RNeasy Mini Kit following the manufacturer's protocol Ribosomal RNA was depleted from the extracted RNA using the Hybridized Probe, rRNA Depletion, and Probe Removal reaction mixtures from the Illumina Stranded Total RNA Prep (Illumina Inc., San Diego, CA, USA). rRNA-depleted samples were purified using a 2.0x sbeadex SAB bead cleanup (Biosearch Technologies, Hoddesdon, UK) with a single 80% ethanol wash. Following cDNA library construction and indexing, the cDNA libraries were quantified using the Qubit™ 1X dsDNA High Sensitivity (HS) Assay Kit (Invitrogen, Thermo Fisher, Carlsbad, CA, USA) and the fragment sizes were assessed using a 4150 TapeStation System (Agilent Technologies, Santa Clara, CA, USA).