Whole Genome Analysis of a Bacterial Isolate from the Gut Microbiota of C57BL6J Mice
The bacterial DNA sample underwent Sanger sequencing targeting the 16S region for initial identification. A single, clear PCR amplicon was purified and subjected to DNA sequencing with specific primers. Subsequently, a paired-end library was prepared from the purified DNA using NEBNext UltraTM II FS Kit, ensuring fragment sizes of 200-300 bp. After quality assessment on Agilent 4200 Tape Station, the library was sequenced on Illumina NovaSeq X Plus for paired-end reads, enabling comprehensive sequencing from both ends of each fragment.