Bulk RNA-Seq analysis of  MC38 colon carcinoma cells and MC38 Bap1-knockout cells in vitro
Bulk RNA-Seq analysis of  MC38 colon carcinoma cells and MC38 Bap1-knockout cells in vitro
Cell viability was detected with 0.4% trypan blue staining solution and counted with Countess II (Invitrogen) cell counter, and the cell viability was over 90%.   RNA was extracted with TRIzol reagent (Ambion 15596-026)follow the instructions RNA was breaken into smart fragments with fragment buffer, and cDNAs were synthesized using the RNA fragments as templates, after which NEBNext Adaptor with hairpin loop structure were ligated ,and 3 µl USER Enzyme (NEB, USA) was used with purified adaptor-ligated cDNA at 37°C for 15 m in followed by 5 min at 95 °C before PCR. Then PCR was performed with Phusion High -Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer.