GSM9087552: Wildtype neurons; Homo sapiens; RNA-Seq
RNA was extracted and isolated using Rneasy Mini plus kit (Qiagen). Libraries for RNA-Seq were prepared with KAPA mRNA-Seq Hyper Prep Kit. The workflow consists of mRNA enrichment and fragmentation, first strand cDNA synthesis using random priming followed by second strand synthesis converting cDNA:RNA hybrid to double-stranded cDNA (dscDNA), and incorporates dUTP into the second cDNA strand. cDNA generation is followed by end repair to generate blunt ends, A-tailing, adaptor ligation and PCR amplification. Different adaptors were used for multiplexing samples in one lane. Sequencing was performed on Illumina NovaSeqX+ for a PE 2x50 run. Data quality check was done on Illumina SAV. Demultiplexing was performed with Illumina BCL Convert v4.2.7 software.