GSM8702339: [RNA-Seq] MCF10A-SATB1-BaP-3d-1 (replica 1); Homo sapiens; RNA-Seq
For RNA-seq: Total RNA was purified by TRI reagent (Sigma) and followed by the RNeasy kit (Qiagen). For RNA-seq: One microgram of total RNA from each sample was reverse transcribed using the QuantiTect Rev Transcription kit (Qiagen). The quality of total RNA was confirmed by 2100 bioanalyzer (Agilent Technologies, Inc.) and high-quality RNA samples (RIN>8) were further processed at QB3-Berkeley Genetics Core Facilities (University of California, Berkeley). After mRNA capture using Oligo(dT)25 magnetic beads (ThermoFisher), the library for sequencing was prepared on Apollo 324TM with PrepXTM RNA-Seq Library Preparation Kits (WaferGen Biosystems, Fremont, CA) according to the manufacturer's recommendation.