NSCLC primary tumor, STK11 WT, KRAS G12D (T cell receptor seq)
Single cell and single nuclei TCR libraries were prepared from amplified cDNA libraries according to manufacturer instructions using the following reagents (all 10X Genomics): Chromium Single Cell V(D)J Enrichment Kit for human T cells (#1000005) was used for cDNA generated with Chromium Single Cell V(D)J reagents (#1000006), and final sequencing libraries were prepared using Chromium i7 multiplexing kit (#120262). Single Cell Human TCR Amplification Kit (#1000252) was used for cDNA generated with Chromium Next GEM Single Cell 5' V2 reagents (#1000263), and final sequencing libraries were prepared using Library construction kit (#1000190) and Dual Index Kit TT set A (#1000215).