GSM8586492: RPE1 cell,sg53BP1-1; Homo sapiens; RNA-Seq
RNA was extracted by phenol/chloroform method. A total amount of 2 μg RNA per sample was used as input material for the construction of sequecing libraries. The clustering of the index-coded samples was performed on a cBot cluster generation system using HiSeq PE Cluster Kit v4-cBot-HS (Illumina) according to the manufacturer's instructions. After cluster generation, the libraries were sequenced on an Illumina platform and 150 bp paired-end reads were generated.