GSM9514766: MG1655 OxyRc single mutant rep. 2; Escherichia coli; RNA-Seq
Three separate cultures each for the MG1655-based strains NR13438 (control), NR13446 (oxyR2c), NR13479 (recA730), and NR13480 (oxyR2c_recA730), were grown o/n in LB at 37 °C. The next day, these cultures were used to inoculate 20 mL of fresh LB medium at a 1:100 dilution and grown until the OD600 of 0.5. The cultures (2 mL) were cooled on ice and harvested for total RNA isolation by centrifugation. The pellets were resuspended in 0.2 mL of lysozyme solution (15 mg/mL in 10 mM Tris-HCl pH 8, 1 mL EDTA) and incubated at RT for 15 min. Total RNA was isolated using the RNeasy Mini Kit (Qiagen; Germany) according to the manufacturer recommended protocol, including the on-column DNAse treatment step. Illumina Truseq Stranded mRNA library kit