Engineered spermidine-secreting Saccharomyces boulardii enhances olfactory memory in Drosophila melanogaster
Engineered spermidine-secreting Saccharomyces boulardii enhances olfactory memory in Drosophila melanogaster
To eliminate/reduce yeast (and yeast RNA) in the intestine, flies were transferred to bottles containing filter paper saturated with 5% glucose in water for four hours. The flies were anesthetized by CO2 and the entire intestine excluding the crop was excised in PBS. Isolated guts were then placed into Eppendorf tubes. Total RNA was isolated from 30 female guts using Qiagenâ€™s RNeasy Plus kit. RNA sample quality assessment was performed with the Fragment Analyzer Standard Sensitivity RNA assay (Agilent) and concentration measured with the Qubit 3.0 HS RNA assay (Thermo).   NGS libraries were prepared with the Stranded mRNA library prep kit (Illumina) using 400 ng of total RNA input. 