RNA-Seq of transfected HEK293T cells with Myc-PRMT5L or -PRMT5S containing plasmids.
We transfected HEK293T cells with Myc-PRMT5L or -PRMT5S containing plasmids. Total RNA was extracted from triplicates of control (non-transfected) and transfected cells, and submitted to McGill University and Genome Quebec Innovation Centre, where RNA samples were reverse transcribed using random primers for library preparation and subjected to protocols for sequencing using Hiseq 2000/2500 sequencer. We obtained 67  1.9 million uniquely mapped reads on average for each sample.