RNAseq of NSCLC patient tissue: K1051.Adjacent Lung
Frozen tissue fragments were lysed and homogenized with the TissueLyser (Qiagen), and RNA extractions were performed with the Qiagen RNeasy Mini Kit. RNA quality was assessed with a 4200 TapeStation system (Agilent Technologies) and samples with an RNA integrity number of more than 8 were selected for library construction. cDNA libraries were prepared with the NuGEN library kit and sequenced with a single/paired- end 2vó75 bp protocol on an Illumina NextSeq 500 instrument.