RNASeq of h.sapiens: female breast cancer
RNA was extracted from ten consecutive 10 m sections from FFPE tissue using the RNeasy FFPE Kit (QIAGEN, Hilden, Germany) following the manufacturer's protocol. RNA concentration and purity were assessed using a Tapestation instrument (Agilent, Santa Clara, USA), and quality control was performed with MiSeq sequencing (Illumina, San Diego, USA). Due to a high level of degraded RNA an amplification step was required. Therefore, RNA libraries were generated through exome target enrichment with All Prep Kit and Exome Panel (Illumina, San Diego, USA). Finally, libraries were sequenced on a NovaSeq platform