GSM9037482: ALS38_parental_1Ery; Staphylococcus aureus; RNA-Seq
Bacterial cells were disrupted by Lysing Matrix B beads on a MP Biomedical FastPrep 24 instrument for 2 cycles of 40M/s.  Cell lysates were extracted with acidic phenol-chloroform (pH 4.7) at least 2-3 times before subjecting to isopropanol precipitation. Crude RNAs were further cleaned up using the Qiagen RNeasy mini columns. Final total RNA were digested with Turbo DNase I to remove genomic DNA contamination Ribo-Zero rRNA depletion and cDNA libraries was performed by NUSeq core (Northwestern Univ) using Illumina Unique Dual Indexes.