WES of Patient 1 Blood
100 ng high quality gDNA sheared by Covaris S2 (target 150 bp). Library prepared using KAPA HyperPrep Kit with end repair, A-tailing, and ligation of IDT for Illumina UD Indexes (10 bp dual index). Size selection with AMPure XP beads (0.8x ratio). Hybrid capture using IDT xGen Exome Research Panel v2. Post-capture amplification with KAPA HiFi HotStart (12 cycles)