GSM9588302: WT; Mus musculus; RNA-Seq
For the single-cell RNA-seq, islets were pooled from three Gcg-Cre;ROSAflox/+ mice and three CBP/p300αDKO;ROSAflox/+ mice at 4 weeks of age. Each group contained 300-400 islets, which were digested with 0.25% Trypsin-EDTA (Sigma-Aldrich) for 5 min at 37 ℃. Digestion was terminated with an equal volume of ice-cold RPMI-1640 containing 10% FBS and the sample was centrifuged at 500 g for 3 min. The suspension was then sieved and the pellet was resuspended in ice cold PBS containing 0.2% BSA. Cell viability and number were assessed by Trypan blue staining and the Eve automatic cell counter respectively. Approximately 10,000 - 15,000 cells for each sample were loaded into the 10× Chromium Chip B according to the 10× Genomics Single Cell 30 Reagent Kit v3 GEM protocol.