GSM9594060: oePsbS_CA1_CA2 , bicarbonate, rep3; Arabidopsis thaliana; RNA-Seq
Total RNA was extracted from Arabidopsis thaliana rosette tissue using the Spectrum Plant Total RNA Kit (Sigma-Aldrich) according to the manufacturer's instructions. Residual genomic DNA was removed by DNase I treatment using the DNA-free Kit (Ambion, Applied Biosystems) for 30 min. RNA concentration was measured using a NanoDrop UV–Vis spectrophotometer (Thermo Fisher Scientific), and RNA quality was assessed using the Experion Automated Electrophoresis System (Bio-Rad). Strand-specific mRNA-seq libraries were prepared from total RNA using the TruSeq Stranded Sample Prep Kit (Illumina) according to the manufacturer's instructions.