RNA sequencing of Citrus sp.: leaflets from adult tree with Citrus tristeza virus
RNA was extracted from leaflets of Citrus sp. with Citrus tristeza virus using the Quick-RNA Miniprep Plus (Zymo Research). RNA was DNAse treated and purified with RNA Clean & Concentrator (Zymo Research). Plant host reads were removed using QIAseq FastSelect-rRNA Plant (Qiagen). cDNA was synthesized using random primers with Maxima H Minus Double-Stranded cDNA and purified with GeneJET Gel and DNA Cleanup (Thermo Fisher Scientific). Sequencing was performed using the SQK-LSK114 (ONT) on a MinION Mk1C using R10.4.1 flow cells (ONT).