Genomic nanopore sequencing of a clonal isolate of Acanthamoeba castellanii Neff transfected with linearized pGAPDH-EGFP - ('Clone LT9')
Genomic DNA was extracted from an axenic monoclonal culture of Acanthamoeba castellanii Neff (ATCC-30010) transfected with linearized pGAPDH-EGFP, using an SDS-based lysis and a phenol-chloroform-based extraction method. DNA was cleaned using a QIAgen G/20 Genomic Clean-up Column. A library was prepared with the Ligation Sequencing Kit (SQK-LSK109) and barcoded with the Native Barcoding Kit (EXP-NBD104). Barcode 9 was used for this sample. DNA was not sheared. Raw reads were basecalled with Guppy 5.1.13 using the SUP model.