GSM8615562: AAI injection induced AKI model sample 2; Mus musculus; RNA-Seq
Kidney tissues from control, AKI, and CKD phase mice were harvested and immediately processed to obtain single-cell suspensions. The kidneys were minced into small fragments and enzymatically digested using a cocktail of enzymes, including collagenase type IV and DNase I, at 37°C with gentle agitation for 30 minutes. The resulting cell suspension was filtered through a 40 μm cell strainer to remove debris and obtain a uniform single-cell suspension. Cells were then washed with PBS containing 0.04% BSA and counted using a hemocytometer to ensure viability above 85% before proceeding to library preparation. Single-cell RNA sequencing libraries were prepared using the Chromium Single Cell 3′ Reagent Kits v2 (10x Genomics) according to the manufacturer's protocol. The library strategy employed was single-cell RNA sequencing (scRNA-seq) using the 10x Genomics Chromium platform.