GSM8957171: Input DNA for 3XFlag-SyCRP1 ChIP-seq under high CO₂ condition; Geminocystis herdmanii PCC 6308; ChIP-Seq
Cells were fixed by formaldehyde at a final concentration of 1%, the fixation was quenched with 125 mM Glycine. Cells were disrupted using glass-beads by vigorous mixing at maximum speed on a vortex mixer. The genomic DNA was fragmented by sonication using Sonics Vibracell, VC505 ultrasonicator. 3XFlag-SyCRP1 was immunoprecipitated with Anti-FLAG M2 affinity gel and eluted with 3xFLAG peptide. Protein and RNA was removed by Proteinase K and RNaseA; the DNA fragments were extracted by PCIA and precipitated by ethanol. The input and immune-precipitated DNA was quantified by Qubit 4 flurometer from invitrogen. The NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®  from NEB was used to prepare libraries. The cleaned libraries were quantitated on Qubit 4 flurometer and appropriate dilutions loaded on a High sensitivity D1000 screen tape on Agilent 4200 tapestation to determine the average size and range of the fragments.