DNBSEQ-T7 paired Sequencing (KAS24111330) (KAE24088074)
Sequencing libraries were generated using DNBseq technology. DNBseq technology consists of DNA NanoBalls (DNBs) which are small, high-density sequencing templates. Rolling-circle replication (RCR) linear amplification makes copies of the original DNA template, instead of copies-of-a-copy. Combinatorial Probe-Anchor Synthesis (cPAS) sequencing chemistry incorporates a fluorescent probe to a DNA anchor on the DNB, followed by high-resolution digital imaging. Libraries were sequenced on a DNBseq-T7 platform with 2 150 bp reads, to a minimum read-depth of 20X.