GSM8774709: MEF, H3K9me3, Rep1; Mus musculus; OTHER
Nuclei were extracted from MEFs using the standard CUT&Tag (Kaya-Okur et al. Nat. Comm. 2019. and https://www.protocols.io/view/bench-top-cut-amp-tag-kqdg34qdpl25/v3) protocol (Nuclear Extraction Buffer). Libraries were prepared for sequencing using standard CUT&Tag (Kaya-Okur et al. Nat. Comm. 2019. and https://www.protocols.io/view/bench-top-cut-amp-tag-kqdg34qdpl25/v3) protocols. i5 and i7 sequencing adaptors were added to isolated DNA via PCR. CUT&Tag with H2A.Z, H3K27ac, H3K27me3 and H3K9me3 antibodies