GSM9510609: NPC__3182_mRNA_R4; Homo sapiens; RNA-Seq
iNPCs and DIV7 iGLUTs were dissociated in accutase, and DIV21 iGLUTs and DIV36 iGABAs were dissociated with papain, washed with and resuspeded in DMEM/10%FBS, and submitted for single cell RNAseq (10X single cell 5' v2 HT with CRISPR barcode kit) in four lanes per cell type, targeting 20,000 cells per lane for a total of ~80,000 targeted cells per cell type. Single-cell libraries were prepared (polyA enriched) using the Chromium Next GEM Single Cell 5' Reagent Kits 10xV2 according to the manufacturer's instructions.gRNA guides were reverse transcribed and amplified using Lentiviral Cas9v2-HygroR specific primers