GSM8740548: HGC-27 cell, control; Homo sapiens; RNA-Seq
Total RNA from HGC-27 and AGS cells and those treated with 10 nM estradiol for 1 day was extracted using TRIzol. The RNA samples underwent stringent quality control through measure the purity and concentration, include agarose gel electrophoresis, NanoPhotometer spectrophotometer, Qubit2.0 Fluorometer, and Agilent 2100 bioanalyzer Libraries were constructed according to the NEB normal method.