Hybridisation capture and sequencing of mitogenome of Solomys ponceleti voucher specimen: adult female liver
Genomic DNA was extracted using the Isolate II Genomic DNA kit (Meridian Bioscience Australia). DNA was sheared with an ultrasonicator before library construction using the NEBNext DNA Library Prep kit. The NEBNext Multiplex Oligos for Illumina were used for adapter ligation and the incorporation of unique indexes via PCR using, Q5 Hot Start DNA Polymerase (New England Biolabs). MyBaits biotinylated RNA hybridisation baits (Arbor Biosciences) were used for target enrichment of mitochondrial DNA. The enriched sample was then amplified using KAPA HiFi (Roche), purified and sequenced with a other multiplexed samples on the Illumina NextSeq 500 platform.