GSM8586723: Sublibary 8; Rattus norvegicus; RNA-Seq
The right and left NG of experimentally naïve, 8-week-old male (n=18) and female (n=18) rats were dissected. rats were deeply anesthetized with 5% isoflurane and sacrificed via rapid decapitation. The right and left NG were dissected and stored in separate PCR tubes before flash-freezing on dry ice. The first side of dissection was counterbalanced across rats. NG samples were stored in a -80 ⁰C freezer until sample preparation. Individual NG were pooled to prepare three replicate groups for each of the four treatment groups (Male|Left; Male|Right; Female|Left; Female|Right). Each replicate group was created by pooling the right or left NG samples from 6 rats. Nuclei were fixed and barcoded according to Parse Biosciences protocols (Evercode Version 2 Nuclei Fixation Kit, Version 2 WT Kit). The samples were split into 8 sublibraries and were sequenced via paired-end sequencing using the Illumina Nextseq 2000 at the Genome Center at the University of Texas at Dallas.