MinION sequencing
PCR-cDNA Barcoding Kit (SQK-PCB114.24) was used for library preparation, according to manufacturer's instruction. Libraries were amplified with 14 cycles of PCR reaction, DNA concentration was measures using Qubit 1xdsDNA high sensivity kit (Invitrogen, Q33231), and then if multiplexed, they were diluted and mixed equally to reach a final concentration of 60-100 fmol.