GSM9590335: hBMSCs, 14D OM+E; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol® reagent (Thermo Fisher Scientific, Rockford, USA). Utilizing an Agilent Bioanalyzer RNA ScreenTape kit (Agilent, 5067–5576), the integrity of the RNA (RIN) was verified. All samples with RIN value > 8.0 were used for sequencing. RNA-seq libraries were prepared using the SRSLY Library Prep kit (Claretbio, CBS-K155B-24) according to the manufacturer's instructions. First- and second-strand cDNA synthesis was performed using the SuperScript III First-Strand Synthesis System (Invitrogen, 18080-051) and E. coli DNA polymerase I (Enzynomics, DP002S), respectively. mRNA was enriched and fragmented using oligo(dT) selection, followed by adapter ligation and PCR amplification.