GSM9267606: hct116_dko_rep3; Homo sapiens; Hi-C
HCT116 Micro-C experiments were placed at 65°C overnight to reverse crosslinking. The following day, phenol:chloroform:isoamylalcohol DNA extraction was carried out followed by RNA removal through RNase A treatment. DNA concentrations were quantified using Qubit. Total DNA was carried over into the final library construction phase, and after amplification, size selection for the appropriate di-nucleosomal band (350-500bp) was carried out with a two-sided cleanup using AMPure XP (Beckman Coulter) beads. Libraries were generated using approximately 1-2 million cells per individual library as described in Maziak N. et al. (in revision).