TCR-sequencing of human: adult female immune cells
Peptides with demonstrated immunogenicity by ELISpot assay were used to stimulate PBMCs or irrelevant peptides as a control. On day 12, cells were harvested, and genomic DNA was extracted and purified from cells using the QIAGEN Blood and Tissue Kit , then TCRV_ CDR3 regions were amplified using the Adaptive Biotechnologies ImmunoSEQ kit then sequenced following manufacturer instructions