sequences of * gene
1.1 Library QC 1. Use Qubit4.0 to quantify the concentration of the library. 2. 2. use Qsep400 to check the integrity of the DNA library fragments and the length of the insert size. insert size meets the expectation, and then proceed to on-line sequencing. 1.2 Sequencing After the libraries have passed the test, different libraries are pooled into flowcells according to the effective concentration and the target downstream data volume, and cBOT clusters are formed. The cBOT was clustered and sequenced using the PE250/PE150/PE300 modes of the Illumina Novaseq 6139/Miseq high-throughput sequencing platform.