RNA-Seq of untreted PeTA MCC cell line
Sequencing libraries were prepared at the Core Facility Genomics (Medical University of Vienna) using the NEB Next small RNA Library Prep Kit according to manufacturer's protocols (New England Biolabs, Ipswich, UK). Libraries were QC-checked on a Bioanalyzer 2100 (Agilent, Santa Clara, CA, USA) using a High Sensitivity DNA Kit for correct insert size and quantified using a Qubit dsDNA HS Assay (Thermo Fisher Scientific, Carlsbad, CA, USA). Pooled libraries were sequenced on a NextSeq500 instrument (Illumina, San Diego, CA, USA) in 1x50 bp single-end sequencing mode. Approximately 15 million reads were generated per sample. Reads in fastq format were analyzed on the Illumina Basespace platform using the Small RNA Sequencing analysis module (version 1.0.1), featuring bowtie aligner (version 0.12.8) and DESeq2 (version 1.0.17).