RNA-Seq of wheat line LP29 inoculated with SN2000  SnTox5 - 04 hpi - Rep 1
Snn5 differential line LP29 was inoculated with Parastagonospora nodorum strains Sn2000 and Sn2000SnTox5, and samples were collected at 0 , 4 , 12, 24, 48, 72, and 96 hours post-inoculation (hpi). More than three such biological replicates were performed. Total mRNA was extracted using Dynabeads mRNA DIRECT Purification Kit (InvitrogenTM, MA), and cDNA libraries were developed using NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, MA).  These libraries were sequenced and 150 bp paired-end sequence reads were generated using the Illumina Hi-Seq X platform at Novogene Corporation Inc. (Sacramento, CA).