GSM9448648: Runx1/p53-null mammary tumor #1; Mus musculus; RNA-Seq
Total RNA was extracted by Zymo RNA extraction kit with Dnase I treatment to remove the genomic DNA Libraries were prepared using Roche Kapa Biosystems RNA HyperPrep sample preparation reagents on a Beckman Coulter Biomek i7. Finished dsDNA libraries were quantified by Qubit fluorometer and Agilent TapeStation 4200. Uniquely dual indexed libraries were pooled in an equimolar ratio and shallowly sequenced on an Illumina MiSeq to further evaluate library quality and pool balance.