Amplicon sequencing of the RT-PCR product upon transfection
The library containing plasmids were then used for transfection, followed by RNA isolation and analysis via RT-PCR using primers and subsequent PAA-gel analysis. For amplicon sequencing, the desired band was excised and purified via the QIAquick Gel Extraction Kit and re-amplified using the same primers. NGS amplicon sequencing was carried out by Eurofins Genomics, Konstanz, Germany using Illumina NovaSeq 6000 PE150.