GSM9513951: Pat.6/Perit._MP 0.2mM; Homo sapiens; RNA-Seq
Total RNA was isolated using silica membrane spin columns (RNeasy Mini Kit, Qiagen, Hilden, Germany) with on-column DNase digestion according to the manufacturer's instructions. RNA quantity and purity were assessed spectrophotometrically, and RNA integrity was verified prior to library preparation. mRNA sequencing libraries were prepared using the TruSeq Stranded mRNA Library Prep Kit (Illumina) according to the manufacturer's protocol. Libraries were indexed, PCR-amplified, purified, and quality-controlled prior to sequencing.