GSM9588978: KO_sample_2; Mus musculus; RNA-Seq
Total RNA was extracted from tissue samples using appropriate extraction methods. RNA quality and concentration were rigorously assessed before library construction. Sequencing libraries were generated using standard protocols. Briefly, mRNA was purified from total RNA using oligo-dT enrichment. Fragmentation was carried out, followed by double-stranded cDNA synthesis. The fragments were subjected to end repair, dA-tailing, adapter ligation, and PCR amplification. Libraries were sequenced on an Illumina platform using a PE150 (paired-end 150bp) strategy.