GSM9589860: Rice mesocotyl under 3 cm sowing depth, biological replicate 1; Oryza sativa; RNA-Seq
Total RNA was isolated from 8-day-old rice mesocotyl using TRIzol reagent (Invitrogen Life Technologies, Carlsbad, CA, USA). RNA quality was assessed using a NanoDrop spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). First-strand cDNA was synthesized using random oligonucleotides and SuperScript II. RNA sequencing libraries were constructed and sequenced using the Illumina NovaSeq 6000 platform.