GSM8841643: MC4100 λlacZ-mEYFP, monosome fraction; Escherichia coli; OTHER
Ribosomes were purifed by sucrose density ultracentrifugation (SeRP total sample), affinity purification (SeRP IP sample) or sucrose gradient ultracentrifugation (DiSP monosome and disome samples). RNA from ribosome fractions was extracted using the hot acid phenol method as previously described (DOI: 10.1038/s41596-019-0185-z) SeRP libraries were prepared as previosly described (DOI: 10.1038/nprot.2013.133). DiSP libraries were prepared as previously publsihed (DOI: 10.1038/s41596-019-0185-z)