16S_DNA_Gut_HiLo
Platform: MiSeq; Paired  end: Yes; Read length (bp) : 250; Primer pair: V4: 515F/806R custom 44 base lead region; DNA polymerase : ThermoFisher Plat II Hot Start MMx2;PCR cycle No. : 35; Adapters:Nextera XT;Sequencing depth  (avg reads per sample): 2086027; Library prep and sequencing protocol:  Earthmicrobiome protocol