Hi-C reads of Lindera aggregata
To prepare a Hi-C library, cells were treated with formaldehyde to bind DNA and protein to be fixed. After cell lysis, the cross-linked DNA was treated with restriction enzymes to create gaps on both sides of the cross-linking point. During end repair, biotin-labeled ends of oligonucleotides were added. Subsequently, adjacent DNA fragments were ligated by treatment with nucleic acid ligase. The proteins at the junction were digested with protease to uncross-link the protein and DNA. Then genomic DNA was extracted and the DNA was randomly broken into fragments with a length of 350 bp using a Covaris crusher for recovery. Finally, sequencing was performed on the Illumina NovaSeq X plus platform in the PE150 model