GSM8879757: Glp1rWnt1-/- Vehicle, Liver, biol rep3; Mus musculus; RNA-Seq
Liver and inguinal white adipose tissue (WAT) collected from DIO GiprSyn-/- and Glp1rWnt1-/- mouse studies with mGIPR-Ab/P1 peptide-antibody conjugate treatment were processed and analyzed using bulk RNA-sequencing. For the extraction of total RNA, tissue samples were pulverized using GenoGrinder (Cole-Parmer), then homogenized in Tri Reagent (Molecular Research Center, Cincinnati, OH, USA or Ambion by Life Technologies) using a MagNa Lyser or TissueLyser II system (Qiagen, Germantown, MD, USA).  mRNA was then chloroform extracted and precipitated using isopropanol and washed with 75% ethanol or precipitated using the RNeasy Plus 96 Kit (Qiagen). RNA was reconstituted with DEPC-treated water. Sequencing libraries were constructed from RNA samples using the TruSeq Stranded mRNA Library Prep kit (Illumina) and IDT for Illumina-TruSeq RNA UD Indexes. Libraries were pooled and sequenced on a NovaSeq X sequencing system (Illumina).