RNA-seq of barley: stem base 0h NPA
Total RNA was extracted from stem base samples. Libraries were prepared using NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). RNA-seq libraries were sequenced on NovaSeq 6000 using S1 flowcell (Illumina) with 2x150 bp reads.