Amplicon sequencing of marine metagenomes
We sampled eDNA from surface and bottom samples across 4 transects with 4 stations each across the Eastern Shore Islands Area of Interest, an area of approximately 2000 km^2 in coastal Nova Scotia. Using a CTD rosette (SeaBird) we collected water samples from the ocean and filtered these on 0.22 micron Sterivex filters to capture eDNA. 12S and 16S amplicons were sequenced in 2021 on a NovaSeq6000 instrument at the Centre for Environmental Genomics Applications in St. John's, Newfoundland.