16S_DNA_Gut_Mix
Platform: MiSeq; Paired  end: Yes; Read length (bp) : 250; Primer pair: V4: 515F/806R; DNA polymerase : Accuprime HiFi polymerase ;PCR cycle No. : 25; Adapters:Integrated in PCR primers;Sequencing depth  (avg reads per sample): 571525; Library prep and sequencing protocol: Illumina 16S Metagenomic Sequencing Library guide and a 2x 300-cycle MiSeq Reagent Kit v3