GSM8613089: Am-HAE1-GFP-RY12; Homo sapiens; OTHER
About 20 w cells were harvested to extract genomic DNA The targeted GFP loci were PCR-amplified from 500 ng genomic DNA using primers listed in the supplementary table. An initial PCR of 25 cycles was performed with TranStart Fastpfu high-fidelity DNA polymerase, and a second round of 15 cycles was used to add sequencing adaptor and barcodes. The final PCR products were gel-purified, quantified by DeNovix, and sequenced by PE150 on NovaSeq 6000 platform (GENEWIZ)