Response of rice treated with glutamine and GS-inhibitors
Response of rice treated with glutamine and GS-inhibitors
Leaf samples were blotted dry and snap-frozen in liquid nitrogen Rice plants were grown in glasshouse under natural light until maturity. Rice leaf discs were mature leaf sections were floated abaxial side down in liquid ½ MS solution (pH 5.7, no sucrose) containing the chemical of interest and incubated for 48h at 28°C, under 24h light. Frozen samples are milled in extraction buffer and total RNA extracted using the QIAGEN Plant RNeasy kit Directional mRNA library preparation (poly A enrichment)