16S sequencing of aquatic biomes near Houston, TX
The V4 region of the 16S gene was amplified using the 515F (5-GTGCCAGCMGCCGCGGTAA-3) and 806R (5-GGACTACHVGGGTWTCTAAT-3) primers with connecting barcodes. PCR products of correct size were separated by electrophoresis (2% agarose). These were then pooled, A-tailed and ligated with Illumina adapters, quantified by qPCR and then sequenced on a Novaseq 6000 and 100K raw reads collected. Sequencing was performed by Novogene (Novogene, China)