Illumina of bacteria:in vitro RumenFermentation
The V3-V4 region of bacterial 16S rRNA gene was amplified using the primers 341F and 805R. In order to conduct multiplexing of samples within a single sequencing run and to assign sequences to samples in bioinformatics analysis, a specific tag was added to both forward and reverse primers. PCR products were purified through Agencourt AMPure XP beads (Beckman Coulter, Indianapolis, USA) according to manufacturers instructions and combined in equal molar concentration to generate a library pool. The 16S rRNA gene library was sequenced at Genomic Platform  Fondazione Edmund Mach (San Michele a/Adige, Trento, Italy) using the Illumina MiSeq system (Illumina, USA).