Plant ITS2 metabarcoding from soil sample ITV21621
PCRs were carried out in 12.5 L volumes with 2 L of genomic DNA and 0.25 L of each primer at 10 M. The annealing temperature was 54C. After PCR, the samples were mixed and purified with Agencourt Ampure XP Beads (Bechman Coulter). Amplicon libraries were prepared following Illumina 16S Metagenomic Sequencing Library Preparation Protocol and then purified. Individual sequencing Nextera XT adapters (Illumina) have been to all samples in a second round of PCRs. The final libraries were sequenced on an Illumina MiSeq platform.