GSM8701411: Control, Naive T cell, scRNA-seq; Mus musculus; RNA-Seq
Lymphocytes from CD4CreTMEM41Bfl/fl mice (KO) and wild-type (WT) littermates were stained with markers for TCRb, CD44, CD62L, and CD25. TCRb was used to identify both CD4 and CD8 T cells, and CD44 and CD62L were used to isolate naive T cells (CD44-CD62L+), while CD25 was used to exclude regulatory T cells. The TCRb+CD44-CD62L+CD25- naive T cells (sorted as the 20% lowest CD44-) from WT or KO mice were sorted for single-cell RNA sequencing (scRNA-seq) analysis. Then naive T cells from WT or KO mice were directly loaded onto a microfluidic chip, and libraries were prepared using the Singleron GEXSCOPETM Single Cell RNA-seq Kit (Singleron Biotechnologies), following the manufacturer's protocol. The sample was processed for scRNA-seq library preparation according to manufacturer's protocol.