Low coverage whole genome sequencing of Neem plant - sample 9a
Qualified DNA samples were taken for Illumina library preparation using TruSeq DNA PCR-FreeLibrary Prep Kit where the genomic DNA is fragmented, end repaired, A-tailed, size selected and ligated with Illumina sequencing barcoded adapters. Concentration of library was checked using QuantStudio Real-Time PCR System and Qubit 4 Fluorometer. After dilution the Agilent 2100 bioanalyzer was used to assess the insert size. he qualified DNA libraries were sequenced.