GSM9626412: ileum lamina propria CD45+ cells, Δchat Bifidobacterium breve-colonized; Mus musculus; RNA-Seq
The lamina propria immune cells were stained with an anti-CD45 antibody and a Near-IR live/dead dye, and live CD45+ cells were sorted. Single-cell RNA-seq libraries were prepared by the Yale Center for Genome Analysis using the standard 10x Genomics Chromium Single Cell Gene Expression protocol. Single cells were partitioned into GEMs for cell barcoding and reverse transcription, followed by cDNA amplification and library construction for Illumina sequencing.