Fungus ITS Sequencing: Phyllostachys praecox
The total DNA of bacteria was extracted from soil samples by CTAB method, and the V3+V4 region of 16S rRNA gene was amplified by specific primers for multiple sequencing. The sequencing Library was constructed according to the NEB NextUltra DNA Library Prep Kit protocol and generated a paired end reading of 270 bp on the Illumina platform.