PRJNA1298177
PCR was per-formed using the Bac_SSU_341F806wR primer pair that targets the bacteri-al small subunit (SSU) ribosomal RNA gene (16S) specifically in the V3V4 hypervariable regions. In Escherichia coli, this primer set amplifies the 341806 region of the 16S. Libraries were prepared using Illumina DNA Prep kit. Sequencing was performed on a NovaSeq 6000 platform, generating 150 bp paired-end reads with a total yield of up to 4 Gb (~1 Gb per sample)