Chitosan activated Biochar in the treatment of winery wastewater
Post-amplification, amplicons were quantified using a Qubit 4.0 Fluorometer with the dsDNA HS Assay kit, pooled in equimolar concentrations, and purified with AMPure PB magnetic beads (Pacific Biosciences, Menlo Park, USA) to eliminate primer dimers and contaminants. The purified amplicon pool was converted into a SMRTbell library utilizing the PacBio SMRTbell Express Template Prep Kit 2.0, adhering to the manufacturer's protocol from pooling to cleanup. Library quality control was conducted using the Agilent 5200 Fragment Analyzer with the HS NGS Fragment Kit (1-6000 bp) to verify insert size distribution and assess adapter contamination. Final library quantification was performed using the Qubit dsDNA HS Assay for optimal sequencing loading concentrations