RNA-Seq of Homo sapiens: THP-1 derived M0 macrophages co-cultured with nanodrug
Total RNA was extracted from THP-1 derived M0 macrophages (induced with 100 nM PMA, co-cultured with nanodrug, and ultrasound-treated) using TRIzol reagent. cDNA was synthesized using [e.g., SuperScript IV Reverse Transcriptase]. Sequencing libraries were prepared using [e.g., NEBNext Ultra II RNA Library Prep Kit] according to the manufacturers protocol, including poly-A selection, fragmentation, adapter ligation, and PCR amplification. Libraries were sequenced on [e.g., Illumina NovaSeq 6000].