KNP17MP70
Total RNA was extracted from homogenized female Aedes aegypti mosquitoes collected near Shingwedzi Rest Camp, Kruger National Park, South Africa. Extraction included a DNase step. RNA was concentrated and reverse transcribed into complementary DNA (cDNA) using random primers. The cDNA was then amplified using sequence-independent single-primer amplification (SISPA) to enrich viral sequences. The resulting PCR products were purified and prepared for high-throughput sequencing on an Illumina platform.