GSM8773117: human endothelial cell Micro-C_sample1round1; Homo sapiens; Hi-C
Using Dovetail  Micro-C Kit following the manufacturer's User Guide. Micro-C assay was performed using Dovetail Micro-C Kit following manufacturer's User Guide for Tissue and Blood to create Micro-C libraries. Briefly, tissues were ground into fine powder with CryoGrinder. The ground tissue was crosslinked with disuccinimidyl glutarate and formaldehyde sequentially. The genomic DNA was digested in situ with MNase Enzyme mix to 20%-40% mononucleosomes.  Genomic DNA was released from cells and put through a sequential process of binding to chromatin capture beads, end polishing, bridge ligation, intra-aggregate ligation, crosslink reversal, and DNA purification. Library was made from the purified DNA and subject to sequencing with Novaseq 6000 platform for approximately 800M 150PE reads.