GSM8587259: 4T1 tumor with High-Fat Diet, rep1; Mus musculus; RNA-Seq
Total RNA was extracted using RNeasy mini kit (Qiagen, CA) and quantified using nanodrop and Agilent bioanalyzer. Total RNA samples  were used for sequencing library preparation according to Illumina stranded mRNA sample preparation guide. Each library was bar-coded and then pooled for sequencing run with 150bp paired-end sequencing protocol.