GSM9450460: peripheral blood mononuclear cells, T1D, sample_17; Homo sapiens; RNA-Seq
Peripheral blood mononuclear cells were isolated from peripheral whole blood samples using standard density gradient centrifugation. Single-cell suspensions were prepared immediately after isolation, washed with phosphate-buffered saline, and assessed for cell viability prior to library preparation. Single-cell RNA-seq libraries were constructed using the 10x Genomics Chromium Single Cell 3' Reagent Kits v3.1 according to the manufacturer's instructions. Briefly, PBMC suspensions were loaded onto the Chromium Controller for gel bead-in-emulsion (GEM) generation, followed by reverse transcription, cDNA amplification, and library construction. Final libraries were sequenced on the Illumina NovaSeq 6000 platform to generate single-cell RNA sequencing data.