Ultra-Longread sequencing by PromethION
Ultra-long Genomic DNA was extracted using the Monarch HMW DNA Extraction Kit for Tissue (NEB) according to the ultra-long DNA Sequencing protocol for SQK-UL114 (ONT). The sizes of the DNA fragments were examined using the Pippin Pulse system (Sage Science). Ultra-long libraries were prepared using an ultra-long sequencing Kit (SQK-UL114, ONT).