GSM8614983: Kelly_shRCOR1_H3K27ac_NT; Homo sapiens; ChIP-Seq
Cells were fixes with 1% formaldehyde for 10 min. After quenching, cells were digested to achieve chromatin fragmented to a range of 150–900 bp using Micrococcal Nuclease. Chromatin samples were immunoprecipitated overnight at 4°C. DNA libraries for ChIP-seq were prepared using NEBNext Ultra II DNA kit following manufacturer's instructions.