HiC sequencing of CYXGZ: leaf
Cells were crosslinked with formaldehyde and DNA was digested with Dpn II (a four-base recognition restriction enzyme). Ends were filled and biotin-labeled before blunt-end ligation. Purified DNA was randomly sheared into 300-500 bp fragments. After library quality control using Qubit 2.0, an Agilent 2100 instrument (Agilent Technologies, CA, USA), and q-PCR, 150 bp paired-end sequencing was performed on the Illumina NovaSeq X Plus platform.