GSM9267319: patient A30, 0 day; Homo sapiens; RNA-Seq
Peripheral blood samples were collected from RA patients prior to TNF-α inhibitor treatment and preserved in PAXgene Blood RNA Tubes (PreAnalytiX). Total RNA was extracted according to the manufacturer's instructions using the PAXgene Blood RNA Kit. High-quality RNA samples were subjected to DNase I treatment prior to library preparation. Strand-specific libraries were constructed using the NEXTflex Rapid Directional mRNA-Seq Kit (BIOO Scientific), following the manufacturer's protocol. Libraries were sequenced on an Illumina NovaSeq platform with paired-end 150 bp (PE150) reads under strand-specific conditions.