ATAC-seq of barley: stem base 9h NAA
Stem bases were fixed in 0.5% methanol-free formaldehyde, snap-frozen in liquid nitrogen and homogenized. Twenty-five thousand G1-phase nuclei were sorted on flow-cytometer and used for library preparation. Libraries were prepared using ATAC-Seq kit (Active Motif) following sample preparation and tagmentation procedures according to manufacturers instructions. Libraries were PCR amplified with Q5 polymerase using NexteraTM-Compatible Multiplex Primers (Active Motif).