GSM9626452: AgedNeu-2; Homo sapiens; RNA-Seq
Total RNA was extracted from the samples. RNA integrity was assessed using the Agilent 2100 Bioanalyzer, and RNA concentration was precisely measured using a NanoDrop spectrophotometer. mRNA was isolated from total RNA using oligo(dT)-coated magnetic beads, and the captured mRNA was subjected to fragmentation. First-strand and second-strand cDNA were then synthesized using reverse transcriptase. The resulting double-stranded cDNA underwent end repair, followed by addition of an adenine (A) base to the 3′ ends. Subsequently, the fragments were ligated to sequencing adapters. After purification to remove unligated products and adapter dimers, the ligation products were amplified by PCR using primers complementary to the adapter sequences. Finally, the sequencing library was purified using magnetic beads.