GSM8702746: Labyrinth, WT_F_Control, Rep4; Mus musculus; RNA-Seq
We homogenized placental tissue using zirconium oxide beads (0.5 mm, ZROB05, Next Advance) and a mechanical shaker (MM300, Retsch). Placental mRNA was extracted using the Qiagen RNeasy Plus kit, including gDNA Eliminator columns to remove genomic DNA, according to manufacturer instructions. Samples were prepped following the standard protocol for the Illumina Stranded mRNA prep (Illumina). Sequencing was performed on the Illumina NextSeq2000 with Paired End 59bp × 59bp reads, with 20 million paired end reads per sample.