GSM8745107: RNA-seq_ESC, E14 WT, rep1; Mus musculus; RNA-Seq
RNA was purified using RNeasy Plus Mini Kit (Qiagen 74136). rRNA was depleted from 1 ug starting total RNA using NEBNext rRNA Depletion Kit v2 (NEB E7405) following manufacturer's instructions. cDNA was synthesized using NEBNext Ultra II RNA first strand synthesis module (NEB E7771) (with random hexamers) and NEBNext Ultra II Directional RNA second strand synthesis module (NEB E7550) following manufacturer's instructions. Libraries were prepared using NEBNext Ultra II DNA Library prep kit for Illumina (E7645S) in conjunction with NEBNext Multiplex Oligos for Illumina (E7600S or E7335S) for barcoding.