GSM9723515: PFC_51 PFC XXSry Control 84051A; Mus musculus; RNA-Seq
RNA was extracted from PFC and NAc tissue punches using the Qiagen miRNeasy Micro Kit (cat# 217084). Libraries were prepared using the Illumina TruSeq mRNA Library Prep kit (cat# 2004053) with 100 ng input RNA and 15 cycles of library amplification. Libraries were sequenced on a NextSeq 2000 UPMC Genome Center on an P3 100 flow cell, 2x50 bp, for an average of ~45 million reads per sample.