GSM9039214: Young sedentary male Fischer-344; biol rep 4; Rattus norvegicus; RNA-Seq
Two days following the final behavioral tests, rats (n = 4 per group) were euthanized, and brains were immediately removed for isolation of hippocampal tissue. Total cellular RNA from the bulk hippocampal tissue was extracted using the Trizol method (Life Technologies). RNA-seq samples were prepared from messenger RNA isolated with NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, catalog no. E7490S) and libraries were generated using the NEBNext Ultra RNA library kit for Illumina sequencers (New England Biolabs, catalog no. E7530S). Five libraries were created for each condition, and 12 pM of each multiplexed library (10 per flow cell lane) were sequenced on an Illumina HiSeq 2500.