GSM9087536: mem B cell, M2_pre_boost_memory, 10x VDJ (BCR); Macaca mulatta; OTHER
PBMCs from immunized Rhesus macaques were thawed. Gross memory (CD27+) B cells were sorted by FACS. Sorted macaque B cells were loaded onto the Chromium X controller for bead formation using the Chromium Next GEM Single Cell v3.1 kit. Cell-barcoded cDNA libraries were generated according to the manufacturer's instruction. VDJ libraries were prepared using the Chromium Single Cell VDJ enrichment mix, using custom primers. Libraries with unique Illumina adapters were pooled and sequenced.