GSM9628942: H3K27Ac_cDC_WT2; Mus musculus; ChIP-Seq
splenic cDCs, splenic pDCs, and bone marrow pre-DCs from 6-8 weeks old Smc3-WT and Smc3-cKO mice were isolated through flow cytometry. RNA was isolated using TRIzol and quantified using tapestation. RNA-seq libraries were prepared using Takara SMARTer® Stranded Total RNA-seq v2 pico input kit following manufacturer's instructions. ChIP-seq libraries were prepared using an inhouse protocol detailed in the methods. Hi-C libraries were prepared using Arima Hi-C+ kit from Arima Genomics. Single cell RNA-seq libraries were prepared using Chromium Single Cell 3′ Reagent Kit targeting 10,000 cells.