captureC-DUXpos
A custom liquid-phase capture method was employed targeting thousands of probes designed for the genes of interest. The Hi-C library underwent adapter blocking to prevent off-target capture. Biotinylated capture probes hybridized to the library, which was then enriched using streptavidin-coated magnetic beads. The captured library was amplified via PCR to generate the final sequencing library. Library quality control was performed using Qubit 2.0 for quantification, Agilent 2100 for insert size analysis, and qPCR for accurate quantification of effective concentration (>2 nM). Qualified libraries were pooled based on effective concentration and desired sequencing depth for Illumina HiSeq PE150 sequencing.