GSM8524446: Inv_SmoM2_SurvivinGOF; Mus musculus; RNA-Seq
We sorted cells for α6High/CD34-/YFP+ from INV-SmoM2 (n=8046) and INV-SmoM2-SurvivinGOF mice. Total10,000 cells were loaded onto each channel of the Chromium Single Cell 3′ microfluidic chips (V2-chemistry, PN-120232, 10X Genomics) and barcoded with a 10X Chromium controller according to the manufacturer's recommendations (10X Genomics). RNA from the barcoded cells was subsequently reverse transcribed, followed by amplification, shearing 5′ adaptor and sample index attachment. The libraries were prepared using the Chromium Single Cell 3′ Library Kit (V3-chemistry, PN-120233, 10X Genomics).