GSM9267387: Wildtype S. pombe cells treated with 75 mM hydroxyurea for 60 minutes.; Schizosaccharomyces pombe; RNA-Seq
Cells were pelleted and ruptured using a precooled mortar with liquid nitrogen. After cell lysis, debris was recovered and snap-frozen in liquid nitrogen. RNAase-free water was then added to the pellets and RNA was extracted using the RNAeasy kit (74104; Qiagen). Final RNA concentration was adjusted to 5 ng and sent to BGI for sequencing. Library construction was performed by BGI using the library type DNBSEQ Eukaryotic Strand-specific mRNA library