GSM8525857: Dual-Smadi; Homo sapiens; RNA-Seq
DIV35 neurons were washed once with D-PBS, lifted using Accutase and RNA was extracted with TRIzol following the provider's instructions. Following isolation, the RNA was treated with TURBO DNA-free kit. Total RNA (1μg) was used per sample for library preparation and sequencing. Chromium Single Cell 3' Reagent V3 kit was used for library preparation according to the manufacturer's specifications. Barcoded libraries were sequenced using the Illumina NovaSeq with 150 bp paired-end reads to an average depth of approximately 3x104 reads per cell.