GSM9588456: mESC IgG - 10K cells - Replicate 3; Mus musculus; OTHER
Genomic DNA of mESCs and OCIAML3 was extracted to perform CUT&Tag using CUT&Tag-direct with CUTAC V.3 (Steven Henikoff, Jorja Henikoff, Kami Ahmad 2021. CUT&Tag-direct with CUTAC. protocols.io https://dx.doi.org/10.17504/protocols.io.bqwvmxe6) CUT&Tag libraries for H3K4me3, H3K27ac, H3K9me3, H3K36me3, RAD21, and IgG were then prepared based on the before-mentioned protocol and sent all mESCs and OCIAML3 samples to the Novogene (illumina Novaseq 6000) for seuencing.