GSM8774721: 230_pre; Homo sapiens; miRNA-Seq
Blood samples were collected into EDTA tubes to prevent coagulation and processed within three hours to isolate peripheral blood mononuclear cells (PBMCs) using Ficoll-Paque (Cytiva) in accordance with the manufacturer's guidelines. The PBMCs were stored in Trizol Reagent (Invitrogen) at -80°C until all samples were collected. RNA was extracted from the PBMCs following the manufacturer's instructions, and eluted in DEPC-treated water. RNA libraries for RNA-seq were prepared using the SMARTer smRNA-Seq Kit for Illumina (Takara) according to the manufacturer's protocol.