GSM9513385: AML12 cultured under standard conditions; Mus musculus; RNA-Seq
The cells were cultured under basal conditions, until they reached approximately 80% confluence. Subsequently they were lysed in guanidine thiocyanate, and total RNA was isolated using a cesium chloride cushion. The isolated RNA was then precipitated with ethanol and resuspended in nuclease-free water. Libraries were prepared according to the manufacturer's instructions for the selected library preparation workflow. Library quality and concentration were assessed, and libraries were normalized, pooled, denatured, and diluted following Illumina guidelines. Sequencing was performed on an Illumina MiSeq using the MiSeq Reagent Kit v2 (300 cycles), generating paired-end reads (2 × 150 bp).