GSM8667527: MN07_gRNA2_Rep1_bulkRNA_hiPS6_LV3822_L1_CRISPRi_batch3; Homo sapiens; RNA-Seq
Total RNA for bulkRNAseq was isolated using the RNeasy Mini Kit (Quiagen) with on column DNAse treatment following the manufacturer's instructions. RNA sequencing was performed using 3 biological replicates. Libraries for RNA sequencing were generated using Illumina TruSeq Stranded mRNA library prep kit (with poly-A selection). The generated libraries for RNA sequencing were optimized for long fragments, and sequenced on a Novaseq6000 (paired end, ~ 150 bp) yielding an average of 46M reads.