GSM8733456: MOCKDAY1, MOCKDAY2, MOCKDAY5, RV1ADAY1, RV1ADAY2, RV1ADAY5, RV+BX795DAY1, RV+BX795DAY2; Homo sapiens; RNA-Seq
To obtain single cells, 3 to 4 rounds of gentle trypsin (TrypLE, Gibco) treatment were performed disassociate ALI culture. Cells were passed though 70µ strainers and fixed according to Parse Biosciences protocol. Counting and viability check using trypan blue were performed before fixation and samples with >80% viability were selected to proceed to library preparation. Parse Biosciences SPLiT-Seq Whole Transcriptomics v1, polyA-selection (currently Evercode(TM)). 8 samples are included in this library: MOCKDAY1, MOCKDAY2, MOCKDAY5, RV1ADAY1, RV1ADAY2, RV1ADAY5, RV+BX795DAY1, RV+BX795DAY2. According to split-seq protocol, 8 samples are pooled into 1 library for sequencing. The full library was sequenced as 8 sub-libraries (not corresponding to 8 samples, each sub-library will contain multiplexed data from all samples).