GSM9267510: 239019031FD; Homo sapiens; OTHER
DNAs were extracted using the DNA FFPE Kit and the QIAamp DNA Blood Kit (Qiagen). Multiplex primers were employed to amplify the CDR3 regions of the V and J genes segments from genomic DNA (gDNA). Paired-end 100 bp PCR products were generated and sequenced on an Illumina platform. TCR-Vβ sequencing was conducted in a CLIA/CAP-certified laboratory (Geneplus), with low-quality sequences filtered out during post-sequencing quality control.