RNA-seq of B. guinensis: radiolar eyes pool
RNA was extracted from a pool of 3 using NucleoSpin RNA XS kit (Machery-Nagel). cDNA was synthesized using the SMART-Seq HT Kit (Takara Bio). Sequencing libraries were prepared using the Nextera XT DNA Library Prep Kit (Illumina) and converted to circular DNA using the MGIEasy Universal Library Conversion Kit. Sequencing was performed on the DNBSEQ-G400 platform (MGI Tech) with a 2x150bp paired-end configuration.