GSM8524520: NDF-degron, MNase_Aux2h_Rep2; Homo sapiens; MNase-Seq
1 million iPSC cells per biological replicate were applied for MNase-seq. The MNase digestion time was optimized beforehand to yield ~80% mononucleosomes and ~20% dinucleosomes. The 140 – 180 bp fragments correspond to mono-nucleosomes were selected with 6% native gel for library construction using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) according to kit instructions MNase-seq libraries were prepared with the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB E7645S) according to kit instructions.