GSM8879404: Papillary renal cancer cells U345, Dasatinib Treated, Rep3; Homo sapiens; RNA-Seq
High-quality RNA was harvested from treated and control cell lines using Qiagen kit. The quality of total RNA was evaluated using the Agilent RNA Screen 2200 Tape Station system (G2964AA). Only samples with a 28S/18S ribosomal peak ratio of 1.8–2.0 and a RIN number >8.0 were considered suitable for sequencing. RNA-seq libraries were prepared using Illumina® TruSeq mRNA Prep Kit RS-122-2101 and were sequenced using Illumina® HiSeq3000/4000 to obtain 76-bp paired-end reads. The sequencing depth for each sample was >20 million reads.