ChIC-Seq, H3k9me3, WT, Untreated, Replicate 1
Chromatin Immuno-capture or CUT&RUN (Cleavage Under Target & Release Using Nuclease) assay was performed using the ChIC/CUT&RUN Assay Kit from Active Motif (cat#53180) and following the  manufacturer instructions. Nuclei were isolated, permeabilized and incubated with the appropriate antibodies (rabbit anti-H3K9me3 [EPR16601](Ab176916, Abcam), rabbit anti-CTCF antibody (active motif 61311)) according to the manufacturers instructions followed with pAG-MNase binding and activation. Digested fragment were finaly solubilized and purified according to the manufacturers. Purifided fragment were used to prepare sequencing library using NEB Multiplex Oligos Kit from Illumina.