RNAseq of SGS2: ASO3scr 150nM
RNA from cells was extracted using the Genezol TriRNA Pure Kit (Geneaid). Total RNA was quantified with NanoDrop Spectrophotometer ND-1000 (NanoDrop Technologies, Inc.) and RNA integrity was assessed through Agilents TapeStation System using the 2100 Bioanalyzer (Agilent Technologies). PolyA enrichment library preparation and sequencing using NovaSeq 600 (100bp paired-end reads) with 2500 million reads was done.