GSM8702900: Creatine-1; Homo sapiens; RNA-Seq
Extract total RNA from cell samples and assess the concentration and purity using the Nanodrop2000. Evaluate the integrity of the RNA through agarose gel electrophoresis. The RNA Integrity Number (RIN) is determined using an Agilent2100. For constructing a single library, a minimum of 1 µg of RNA is required, with a concentration of at least 50 ng/µL and an OD260/280 ratio between 1.8 and 2.2. 1.5μg of total RNA was used for the construction of sequencing libraries. The RNA library for sequencing was meticulously prepared in accordance with the manufacturer's guidelines using the TruSeq™ RNA Sample Preparation Kit.