GSM8667781: B6-LPS-M2; Mus musculus; RNA-Seq
Samples were thawed, pelleted at 500xg, and lysed in 700µL QIAzol. RNA was extracted using the miRNAeasy isolation kit (Qiagen). DNA was depleted using TURBO DNase (Invitrogen AM2238). DNase was removed and samples were concentrated using the Monarch® Spin RNA Cleanup Kit (New England Biolabs T2050S). Samples were submitted to Azenta Life Sciences/Genewiz for RNA quality assessment, library preparation, and sequencing (Illumina NovaSeqX+ 2x150bp). Ribosomal RNA were depleted by polyA enrichment.