RNA-Seq of control S. aureus MW2
We started with exponential cultures of S. aureus MW2 grown in TSB medium and adjusted to OD600 = 0.4. We treated 10 ml of this bacterial culture with 0.5 MIC of peptide or water as a negative control for 30 minutes at 37C with shaking at 180 rpm. Following treatment, we washed the bacteria three times with PBS and stored the pellet at -80C. We extracted total RNA from the pellet and performed RNA sequencing studies, we adhered to the protocol recommended by Novogene (Sacramento, CA, USA).