GSM9266982: Single-cell RNA-seq retina (Taeniopygia guttata), biological replicate 4; Taeniopygia guttata; RNA-Seq
Retinas were dissected from freshly euthanized adult animals. For Visium libraries, eyes were embedded in OCT, cryosectioned (10 µm), and mounted on 10x Genomics Visium slides. For Chromium single-cell libraries, retinas were enzymatically and mechanically dissociated into single cells following the 10x Genomics recommended protocol. Spatial transcriptomics libraries were prepared using the 10x Genomics Visium Spatial Gene Expression kit v1 (poly-A capture, 55 µm spot resolution). Single-cell RNA-seq libraries were prepared using the 10x Genomics Chromium Single Cell 3′ v3 chemistry. All libraries were sequenced on an Illumina HiSeq 4000 with paired-end 2 × 150 bp reads.