GSM8773199: CMO multiplexing library on WT and Hox-/- gastruloids at various stages; Mus musculus; RNA-Seq
Control and Hox-/- gastruloid at 72h, 96h and 120h were collected, dissociated and processed. Samples were processed following the manufacturer's instructions of the 3' CellPlex Kit (10X Genomics). Cells were then pooled and subjected to single-cell RNA-seq using the 10x Genomics platform (V3.1 chemistry). cDNA preparations were performed according to 10X Genomics recommendations, amplified for 10–12 cycles and sequenced on a Novaseq 6000 with the cbot2 chemistry.