DNA-seq of a single Chrysomya bezziana male
Genomic DNA was extracted from a single frozen male fly using the CTAB method (Hunt 1997). The DNA was initially quantified using Nanodrop 2000 (Thermo Scientific) and Qubit dsDNA BR Assay Kits. DNA integrity was assessed with 4150 TapeStation (Agilent). DNA was fragmented to match the expected insert size (150bp). A PCR was conducted to amplify the library and to generate the full-length adapter containing a unique index sequence in each library sample. The library samples were sequenced with 300 cycles (150-PE) using Illumina NextSeq 500.