illumina iSeq100 paired sequencing  (KAS24163882) (KAE24105907)
To enhance the efficiency of Cas-based 218, purified genomic DNA samples were fragmented. The generated library fragments predominantly ranged from 150 to 250 bp in size. Size-selected DNA fragments were amplified by PCR and subsequently purified, and each library was sequenced using the Illumina MiSeq platform. The sequencing process produced raw data for each sample in the form of paired-end reads, which were stored in FASTQ format.