GSM8702590: TYK-nu_ZNFX1_KO_Biological_Replicate_1; Homo sapiens; RNA-Seq
RNA was isolated from cell pellets using the RNeasy kit according to manufacturers instructions (Qiagen). Quality and quantity of RNA was monitored using the Bioanalyzer RNA High Sensitivity kit on a Model 2100 BioAnalyzer (Agilent). All RNA samples had a RIN score of 9 or greater. 500 ng of total RNA was used for library construction with the TruSeq Stranded Total RNA Library kit according to manufacturers instructions (Illumina). Quality and quantity of the resulting cDNA was monitored using the Bioanalyzer DNA High Sensitivity kit (Agilent). Bulk RNA libraries were sequenced on an Illumina Novaseq 6000 X instrument using 150bp paired-end dual indexed reads and 1% of PhiX control. Depth of coverage was targeted to a total of 50 million reads (25 million pairs) per library.