RNA Sequencing of LLOMe treated MEF cells
RNA Sequencing of LLOMe treated MEF cells
Untreated and treated MEF cells were collected from 3 different 10cm plates for each group  RNA was extracted from MEF cells (three biological replicates) using RNeasy mini kit (QIAGEN, #74104). The quality and quantity of total RNA was checked using agarose gel and Qubit 4.0. After assessing the quality of RNA, 800-900 ng of total RNA was subjected to NEBNext® ultra™ directional RNA library prep kit for Illumina® (#E7420) using NEBNextPoly(A) mRNA magnetic isolation module (NEB #E7490). The QC of the prepared library was performed using High-Sensitivity Tape Station Kit (Agilent 2200, 5067-5585 (reagents) #5067-5584 ( screen tapes)) for fragment length distribution and Qubit dsDNA HS assay kit (Invitrogen, Q32851) for quantifying the library.