GSM8585885: THLE-3 cells, ICR, combined, oAs + CdCl2, rep3; Homo sapiens; Bisulfite-Seq
The gDNA was extracted using the QIAamp DNA Mini Kit (QIAGEN, Hilden, Germany) following the manufacturer's protocol Bisulfite modification of the 2 μg of gDNA was performed using the EZ DNA methylation-lightning kit. The bisulfite-converted DNA was amplified to a 387-bp sequence element harboring 21 CpG sites located at the H19 DMR and a 348-bp element involving 17 CpG sites at the ICR using TaKaRa EX Taq DNA polymerase. The library was prepared according to Illuminaâs recommendations and sequenced using the 300-cycle MiSeq reagent kit V3 (paired end, 2 Ã 300 bp) on an Illumina MiSeq instrument (Illumina Inc., San Diego, CA, USA) for BSAS analysis.