GSM9282616: Dox + rep1 (MS330_010); Mus musculus; RNA-Seq
FACS-sorted cells were lysed, and total RNA was purified with the RNeasy Micro Kit (Qiagen, #74004) according to the manufacturer's instructions. cDNA synthesis and library construction followed the SC3-seq protocol (Nakamura et al, 2015; Mizuta et al, 2022). For each sample, 1 ng of total RNA was used for cDNA synthesis, and cDNA was amplified for 14 PCR cycles—the established cycle number for bulk RNA-seq. Libraries were prepared from the amplified cDNA and sequenced on the Illumina NextSeq 500/550 platform (High Output Kit v2, 75 cycles; Illumina, cat. no. FC-404-2005).