GSM8743663: Norm_Ctrl 2; Mus musculus; RNA-Seq
Microglia were isolated from single-cell suspensions of mouse brains using the CD11b MicroBeads and magnetic-activated cell sorting (MACS) (Miltenyi Biotec) to ensure purity. RNA extraction was performed using the RNeasy Plus Micro Kit following the manufacturer's protocol. RNA quality was assessed using the Bioanalyzer 2100 system (Agilent Technologies), and all RNA samples had a RNA integrity number (RIN) greater than 6.8. The cDNA library was prepared using the NEBNext Ultra Directional RNA Library Prep Kit (New England BioLabs).