GSM8493846: vegetative wt cells, total RNA, biol rep 2; Schizosaccharomyces pombe; RNA-Seq
Total RNAs were extracted using the hot acid phenol method. Total RNAs were subjected to ribosomal RNA depletion and directional RNA-seq librairies were constructed using the TruSeq Stranded Total RNA library prep Gold kit (Illumina, #20020598). PolyA RNAs were enriched from total RNAs using oligodT-attached magnetic beads and mRNA librairies were constructed using the Novogene NGS RNA Library Prep Set (PT042).