GSM9514783: 2C3_a; Arabidopsis thaliana; Bisulfite-Seq
Per sample, frozen plant tissue was grounded with liquid nitrogen and genomic DNA was isolated using the SDS procedure of the NucleoSpin Plant II DNA isolation kit (MachereyNagel, Germany). A NanoPhotometer® spectrophotometer (IMPLEN, CA, USA) and agarose gel electrophoresis were used to determine DNA concentration and integrity after the genomic DNA extraction. DNA libraries were prepared for bisulfite sequencing using the NEBNext® Enzymatic Methyl-seq kit (NEB, Ipswich, MA, USA).