GSM9037889: MPNST10; Homo sapiens; RNA-Seq
RNA from fresh frozen sarcoma patient samples or patient-derived cells was extracted using the Maxwell RSC simplyRNA Tissue kit (Promega AS1340). The extracted RNA was quantified with a Nanodrop (ThermoFisher Scientific) and the High Sensitivity RNA ScreenTape Assay for TapeStation systems (Agilent 5067). RNA sequencing libraries were prepared using the Illumina Stranded mRNA sample preparation protocol