GSM8701880: RNATCRseq_aPDL1_GP33pos_Lane1_RNA; Mus musculus; RNA-Seq
At 102 days post-infection, splenocytes were enriched for CD8+ T cells using magnetic separation and stained with sample-specific LMO, viability dye, GP33 tetramer, and surface antibodies. Live CD8+ Tetramer+ T cells were sorted into RPMI supplemented with 10% FBS. After sorting, samples were pooled and loaded into the Chromium Controller (10x Genomics). scRNA and TCR libraries were prepared according to manufacturer's instructions (10X Genomics 5' v2). MULTI-seq library preparation workflow performed following cDNA amplification, as described previously (McGinnis, Patterson, et al., Nature Methods, 2019).