GSM8526323: NR1241Br; Mus musculus; RNA-Seq
TRIzol extraction followed by DNaseI treatment and RNA column cleanup mRNAseq libraries were generated from 500 ng of high-quality total RNA, as assessed using the Bioanalyzer (Agilent), according to the manufacturer's directions using a 5-fold dilution of the universal adaptor and 9 cycles of PCR per the respective masses with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7760L), the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490L), and the NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (NEB, E6440S) and purified using the SPRIselect bead-based reagent (Beckman Coulter, B23318).