GSM9258115: St55_PHP41_transcript_library1; Pleurodeles waltl; RNA-Seq
Tissue pieces were dissociated for 30 min at room temperature using the Papain dissociation kit (Worthington) in calcium-free Hibernate A (BrainBits). After processing and concentration, cells were resuspended in calcium and magnesium-free HibernateA (BrainBits) at a concentration of 1000 cells/µl. Cells were counted in a haemocytometerchamber and immediately processed for single-cell GEM formation (10x Genomics, single cellRNA sequencing 3', Chromium V3.1). Cells  were resuspended at ~1500 cells/µl and multiplexed using 4 distinct Cell Multiplexing Oligos from the 10x Genomics 3' CellPlex Multiplexing kit prior to GEM formation The cells were loaded into a 10x Chromium Chip G with a targeted cell recovery of 5000- 8000 cells for GEM Generation and cell barcoding. Single-cell RNA-seq libraries were prepared with 10x Chromium Next GEM Single Cell 3' Reagent Kit v3.0 or v3.1 (Dual Index)