RNAseq of SCC1 Control
RNA extraction from SCC1 cells was conducted, with RNA purity quantified by Nanodrop and integrity assessed via Agilent 4200 TapeStation. Libraries were constructed from 1 g RNA through the following protocol: mRNA underwent fragmentation using divalent cations, followed by first-strand cDNA synthesis with reverse transcriptase and second-strand synthesis using DNA polymerase I/dNTPs. Double-stranded cDNA was processed through end repair, A-tailing and adapter ligation (200-300 bp size selection). PCR-amplified products were purified for final libraries, which were sequenced on NovaSeq 6000 (LC-Bio) after quality control.