GSM8701876: scRNATCRseq_GP33_Lane3_LMO; Mus musculus; OTHER
CD8+ T cells were enriched using magnetic separation and stained with sample-specific LMO, viability dye, GP33 tetramer,and surface antibodies. Live CD8+ GP33+ T cells were sorted using a BD FACSAria into RPMI supplemented with 10–50% FBS. Sorted cells were pooled and loaded into the Chromium Controller (10x Genomics). Naïve T cells were also sorted as controls. In batch 2, samples were further divided based on CD62L expression,; batch 1, total GP33+ cells were sequenced. Each batch was processed independently, with separate library preparation and sequencing. scRNA and TCR libraries were prepared according to manufacturer's instructions (10X Genomics 5' v2). MULTI-seq library preparation workflow performed following cDNA amplification, as described previously (McGinnis, Patterson, et al., Nature Methods, 2019).