EU-RNA-Seq of METTL4 KO HeLa: 20 minutes after labeling
HeLa S3 METTL4 double knockout cell culture was obtained using CRISPR-Cas9 technique. Next, it was incubated with 5-ethynyl uridine (EU) for 20 minutes. Total RNA was extracted using QIAzol lysis reagent. Nascent RNA labelled with EU was purified using Click-iT Nascent RNA Capture Kit. NEB NEBNext Ultra II RNA Library Prep Kit for Illumina (E7770) was used for library preparation.