GSM9282317: iPSC_EC_Control_4; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNeasy Plus Mini Kit (Qiagen). mRNA was purified using Dynabeads Oligo(dT) (Life Technologies) and fragmented into 200-300 bp segments for preparation of index-tagged paired-end cDNA libraries. Illumina sequencing adapters were ligated to cDNA fragments during PCR amplification, and libraries were sequenced on an Illumina HiSeq platform.