GSM8880353: iMOP-derived neuron_1, RNA-seq; Mus musculus; RNA-Seq
Total RNA was extracted from 1-5x10^6 proliferating or neuronal iMOP cells using 1ml of TRIzol Reagent (Thermo Fisher Scientific). Total RNA was treated with DNaseI, and then DNaseI was removed by phenol-chloroform extraction. Ribosomal RNA was depleted from 500 ng of DNase-treated total RNA using the NEBNext rRNA depletion kit. Stranded RNA-seq library was prepared with the Takara SMARTer Stranded RNA-seq kit.