GSM8957000: GBM8205,TMZ1; Homo sapiens; RNA-Seq
Total RNA was extracted using the TRIzol reagent (15596-026, Invitrogen, USA) and purified using an RNA-Quick Purification Kit (Yishan Biotechnology Co., Ltd., Shanghai, China). The transcriptome library was constructed following the standard protocol for VAHTS Universal V5 RNA-seq Library Preparation Kit (NR616-01/02, Vazyme, Nanjing, China).