ITS of termite gut microbiota: rubber plantation
Termite gut DNA was extracted using Qiagen DNeasy PowerSoil Kit (Qiagen, Germany), following the manufacturers protocol. The initial vortex step was replaced by a bead-beating step at 30 Hz for 10 min using Retsh MM400 (Mikro-Dismembrator). Fungal ITS was amplified using  gITS7 (fungal-specific primer) (5'-GTGARTCATCGARTCTTTG-3') and ITS4ngs (5'-TTCCTSCGCTTATTGATATGC-45').