16sDNA sequence of Staphylococcus gallinarum
The PCR products were run on a gel and cleaned up enzymatically using the EXOSAP method.The extracted fragments were sequenced in the forward and reverse direction(Nimagen, BrilliantDye Terminator Cycle Sequencing Kit V3.1, BRD3-100/1000) and purified (Zymo Research, ZR-96 DNA Sequencing Clean-up Kit, Catalogue No. D4050). The purified fragments were analysed on the ABI 3500xl Genetic Analyzer (Applied Biosystems, ThermoFisher Scientific) for each reaction forevery sample, as listed in Section 1. BioEdit Sequence Alignment Editor version 7.2.5was used to analyse the. ab1 files generated by the ABI 3500XL Genetic Analyzer and results were obtained by a BLAST search (NCBI).