GSM8586382: Mk, Old, Rep2; Mus musculus; ssRNA-seq
oligodt-selected mRNA RNA was treated on column with 0.5 kunitz units of DNAse prior to elution according to manufacturers instructions. RNA from Young and Old FACS-isolated Mks  was extracted. Messenger RNA was captured using oligodT Dynabeads (Life Technologies). Strand-specific RNA-seq libraries were constructed using a TruSeq Stranded mRNA library Prep Kit, following the manufacturers instructions (RS-122-2101)