Whole-exome sequencing of Homo sapiens: Kaposi Sarcoma tumor and matched normal skin
Genomic DNA was extracted from 4x4 mm punch biopsies of Kaposi Sarcoma tumors or matched normal skin using the Qiagen DNeasy kit. Exome libraries were prepared from 200 ng of sheared DNA (~400 bp fragments) using Agilent SureSelect v6 hybrid capture and sequenced using paired-end reads (150 bp on HiSeq 4000, 100 bp on HiSeq 2500 and NovaSeq 6000). Libraries were validated with Agilent TapeStation and quantified using a Qubit fluorometer.