Genome sequencing of African ASFV isolates
ASFV DNA was extracted using the MagAttract high molecular weight DNA kit (Qiagen) from concentrated DNAase treated virus grown in porcine bone marrow cells. Viral DNA was then amplified using REPLI-g (Qiagen) and sheared with T7 endonuclease. The presence of ASFV DNA was confirmed by qPCR (King et al., 2005) and only DNA with > 1E6 genome copies were used to generate libraries with approximately 2000 ng DNA.