RNA sequencing of CYXGZ: stem-2
RNA samples passing quality control were enriched for eukaryotic mRNA using Oligo(dT) magnetic beads. The enriched mRNA was fragmented and utilized as a template for first-strand cDNA synthesis with random hexamer primers. During second-strand synthesis, dATP, dCTP, dGTP, and dTTP were incorporated to construct the double-stranded cDNA library. The resulting dsDNA was purified, followed by end repair and adenylation (A-tailing). Sequencing adapters were then ligated to the repaired ends. Fragments of target size were selected, and the cDNA library was amplified via PCR. Finally, the amplified library was sequenced on the Illumina NovaSeq X Plus platform.