RNA-Seq of mus musculus: adipose
Total RNA was extracted using by Meige Genetics BioTech Co., Ltd. (Guangzhou, China). RNA degradation and contamination were monitored by electrophoresis on 1% agarose gels. RNA concentration was measured using Qubit 4.0 (Thermo Fisher Scientific, Waltham, USA) and Nanodrop One (Thermo Fisher Scientific, MA, USA). RNA integrity was accurately assessed using the Agilent 4200 system (Agilent Technologies, Waldbronn, Germany). Sequencing libraries were generated using ALFA-SEQ RNA Library Prep Kit (Cat. NRI002E-03 ).