16S rRNA gene amplicon sequencing of Archaea: heated water sample
Total genomic DNA extractions from single water droplet were carried out using AxyPrepTM Bacterial Genomic DNA Miniprep Kit (Axygen Biosciences, CA, USA). The archaeal 16S rRNA genes were PCR amplified using nested PCR primers of Arch340F/Arch1000F and Uni519F/Arch806R.