surface ice WGS
Covaris E220 sonicator was used to generate 300-400 bp fragments, then  libraries were constructed using the NEBNext UltraTM II DNA Library Prep Kit for Illumina (Cat# E7645S) and barcodes were added with the NEBNext Unique Dual Index Oligos kit (Cat# E6440S). Paired-end sequencing was conducted on the NovaSeq6000 Illumina Sequencing 2x150 v2 platform.