GSM8666012: gcn4∆,H2O2,Replicate3; Nakaseomyces glabratus; RNA-Seq
The RNA was extracted by utilising a hybrid method combining the classic acid-phenol extraction method (Schmitt et al., 1990). Then, finally, eluting using an RNA extraction kit by Qiagen (Cat. No. 74104).The extracted RNA quality was verified via gel electrophoresis, qRT-PCR, Qubit fluorometer (Thermofisher #Q33238) using RNA HS assay kit (Thermofisher #Q32851), Nanodrop 1000 and RIN values from an Agilent screen tape station. The library preparation used Trueseq standard total RNA (Illumina #20020597). Similarly, the final libraries were quantified using a Qubit fluorometer, and the tape station measured the insert size. The prepared library was sequenced by paired-end sequencing on an Illumina platform.