GSM8615084: 399, scRNAseq; Homo sapiens; RNA-Seq
Samples were collected from cryopreserved human lung samples in 10%DMSO. After thawing at 37*C, the tissue was minced into small pieces, incubated for 45min at 37*C with DMEM + Elastase, DNAase, liberase and 1% penicillin+streptomycin+glutamine. Digestion was stopped with fetal bovine tissue ato 10%. The digested tissue was passed on a 100micron mesh and later on centrifuged at 300g for 10 min. Cell pellets were resuspended with MACS medium, and later on the cells were barcoded using the 10x chromium single cell platform. The samples were processed either with the Chromium X or the Chromium Controller, using the Single cell 5' v2, or Single Cell 5' v2 HT kit. Libraries were sequenced using the Illumina NextSeq 500 system.