GSM9589808: mack2_p180_1; Scomber scombrus; RNA-Seq
RNA was extracted from mack cells using the RNEasy Mini kit (Qiagen #74104, Hilden, Germany) according to the manufacturer's directions. RNA was extracted for three experimental replicates for each Mack1 condition and four experimental replicates for each Mack2 condition. RNA samples were sent on dry ice to Genewiz for library preparation and RNA sequencing. RNA samples were quantified using the Agilent 2100 Bioanalyzer. mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by second strand cDNA synthesis. The library was prepared using a non-directional (non-strand-specific) protocol. Final libraries were quantified by QPCR and sequenced on an Illumina NovaSeq X Plus platform (PE150).