Oxford Nanopore amplicon sequencing of Lolium perenne: CO, FT3, piVRN1 genes
Target amplicons were generated by PCR from genomic DNA of individual 51 from F2 A25012 population. PCR products were barcoded using Oxford Nanopore native barcode NB96, multiplexed on R9.4.1 MinION flow cells, and sequenced. Reads were basecalled and demultiplexed using Guppy v6. Within-read and residual terminal adapters were trimmed using Duplex Tools and Porechop respectively prior to submission