GSM8775516: BMM_ATAC-seq; Mus musculus; ATAC-seq
Take out the cryovial and shake it in 37℃ water to quickly thaw it within 1-2 min.Cells were centrifuged at 4 ℃ 500×g for 5 min. Cell activity was detected with LUNA-FL and counted.50000 live cells were centrifuged with 500 g for 5 minutes, supernatant was discarded, The precipitation was resuspended with 50 ul lysis buffer,and placed on ice for 5 minutes, then 1ml wash buffer was added, mixed, 500 g centrifuged for 5 minutes, supernatant was discarded, and nucleus was collected. Briefly, nucleus was extracted from samples, and the nucleus pellet was resuspended in the Tn5 transposase reaction mix.The transposition reaction was incubated at 37 ℃ for 30 min. Equimolar Adapter1 and Adatper 2 were added after transposition, PCR was then performed to amplify the library(ATAC-seq_Novogene Kit). After the PCR reaction, libraries were purified with the AMPure beads and library quality was assessed with Qubit