pE022
Plasmid DNA was extracted using the Zymo Research Midiprep kit and sequenced on an Oxford Nanopore GridION platform. The sequencing library was prepared using the Native Barcoding Kit 24 V14 (Oxford Nanopore Technologies), with plasmidic DNA as the library source. This approach enabled multiplexed long-read sequencing to obtain high-quality data for accurate plasmid assembly and characterization.