RNAseqofA549cell cultured in soft ECM
Bioprinted samples were first digested to collect the cells. 2D cultured cells were digested from culture flasks and centrifuged to collect a pellet. Trizol was immediately added to cell pellets to preserve RNA integrity. Total RNA was extracted using Zymo Microprep kit. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.