T1
The library construction was carried out using the VAHTS Universal Plus DNA Library Prep Kit for Illumina (ND617). The following steps were followed according to the kit instructions. Use Qsep-400 to assess the library fragment, use Qubit 3.0 to quantify its concentration. The following quality indicators need to be satisfied for the library to proceed to sequencing: concentration  1 ng/L, center fragment size of 430-530 bp, average fragment size between 420-580 bp, a Gaussian distribution of peaks, and the absence of any additional peaks. The constructed library will be sequenced using the Illumina NovaSeq sequencing platform, with a PE150 sequencing mode.