GSM8878561: h1_ut_d14_rep2; Homo sapiens; ATAC-seq
50,000 hESC-HSPCs were processed as follows: nuclei were isolated by lysing with 50 ul of ATAC lysis buffer (10 mM Tris pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% NP40, 0.1% Tween-20, and 0.01% Digitonin) and washing with 1 mL of ATAC wash buffer (10 mM Tris pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% Tween-20). Cell lysates were spun to obtain nuclear pellets, which were subjected to transposase reaction for 45 minutes using the Illumina Nextera DNA Sample Preparation Kit according to the manufacturer's instructions. The final libraries were quantified using the Agilent BioAnalyzer. Sequencing of 75 nucleotide-long paired-end reads was performed in a NextSeq-500 (Illumina).