Amplicon of strawberry rhizosphere soil: Autoclaved slurry suspension, H2O suspension, Mite-infested, Replicate 3
DNA was extracted from rhizosphere soil using the E.Z.N.A soil DNA kit (Omega Bio-tek). The bacterial communities were analyzed by amplifying a 440 bp fragment of the V3-V4 region of the bacterial 16S rRNA gene using the primers 341F-806R while the fungal communities were analyzed by amplifying a 380 bp fragment of the ITS2 region using the primers ITS3 and ITS4. PCR amplification, library preparation, DNBSEQ sequencing (2x300 bases paired-end version), demultiplexing and barcode suppression in our rhizosphere soil samples were performed by BGI Tech Solutions (Hong Kong, China). Cutadapt was used to remove the primers from the sequences. The sequences uploaded on NCBI are demultiplexed and trimmed from barcodes and primers.