GSM8773159: human arteriole _4; Homo sapiens; Bisulfite-Seq
Genomic DNA of tissue/cell was extracted following Diagenode's Premium RRBS protocol (https://www.diagenode.com/). RRBS libaries wer made following Diagenode's Premium RRBS protocol (https://www.diagenode.com/). In brief, genomic DNA was digested using Msp1, followed by end-repair. Next, premium Methyl Unique Dual Indexes (UDI) and Unique Molecular Identifiers (UMI) Adapters were ligated to the resulting fragments. Then, UDI-UMI-tagged fragments are size-selected to enrich CpG-rich regions with gene regulatory potential. Finally, the enriched DNA underwent bisulfite conversion, followed by sequencing on an Illumina Novaseq 6000 platform with paired-end 50 bp reads