Sample4
The extracted DNA was fragmented to an average size of approximately 400 bp using the Covaris M220 (Gene Company Limited, China) for paired-end library construction. A paired-end library was constructed using the NEXTFLEXTM Rapid DNA-Seq (Bioo Scientific, Austin, TX, USA), followed by sequencing on the Illumina HiSeq 2503 platform using NovaSeq Reagent Kits.