RNA-seq of E13.5 WT and Emb-/- kidney
RNA-seq of E13.5 WT and Emb-/- kidney
E13.5 WT (four individuals per replicate) and Emb-/- (six individuals per replicate) kidneys were dissected Total RNA was isolated by using the NucleoSpin RNA kit (Macherey-Nagel) according to the manufacturerâ€™s instructions.  The libraries were prepared from 300 ng of RNA from each sample using TruSeq Stranded mRNA HT Kit and TruSeq Stranded mRNA Sample Preparation protocol 15031047 (Illumina) 