GSM9589149: T1R2; Canis lupus familiaris; RNA-Seq
Bio-rad Aurum™ Total RNA Mini Kit Poly(A) mRNA enrichment was performed from total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Stranded RNA-seq libraries were prepared using the NEBNext UltraExpress RNA Library Prep Kit (New England Biolabs) following the manufacturer's instructions. The libraries were sequenced on an Illumina NovaSeq X Plus platform with 101 bp paired-end chemistry.