RNA-seq from mouse ears infected with L. major and colonized with S. aureus at day 7 post-infection
Tissue was first cryo-pulverized using the CP02 CryoPrep automated dry pulverizer (Covaris). Trizol (Invitrogen) based phenol-chloroform extraction was then performed for RNA purification, followed by on-column DNase digestion (Qiagen). Illumina/NebNext Stranded mRNA was used for poly-A enrichment.