DNA Panel of Homo Sapiens: SCLC Blood
The library was constructed using the NEBNext Ultra II DNA Library Prep Kit for Illumina (cat #E7645, New England Biolabs, Ipswich, MA, USA). DNA samples were fragmented using Covaris M220 (cat #500295, Covaris, Woburn, MA, USA), followed by end repair, A-addition, adapter ligation, and amplification. Targeted regions were captured using the Med1CDxTM Panel (cat #N020B01, MEDx Translational Medicine, Suzhou, Jiangsu, P. R. China). Sequencing was performed on the Illumina NovaSeq 6000 system (cat #20012850, Illumina, San Diego, CA, USA), producing paired-end reads of 150 bp.