GSM8667065: B6_Plate 8A; Mus musculus; RNA-Seq
Dorsal pancreatic tissue (E12.5) was dissected from Tg(Prox1-EGFP), Tg(Nkx2.5-Cre;mTmG) and Tg(Nkx3.2-Cre;mTmG) transgenic mice and dissociated into single-cell suspensions. GFP+ cells were FACS-sorted at 4°C using an Aria II or Aria III flow cytometer (BD) into 96-well plates containing cell lysis buffer. Plates containing FACS-sorted cells were then frozen and kept at -80°C until shipment to the Genomic Facility for library preparation and sequencing. Libraries were prepared for sequencing using Nextera XT DNA library preparation kit (Illumina) protocols.