GSM9359417: Tumor, conventional 7x19 CAR-T, 2; Mus musculus; RNA-Seq
CD45+ cells were enriched using the CD45 EasySep positive selection kit, followed by depletion of CD90.1+ cells. Total RNA was extracted from the remaining recipient immune cells. RNA libraryies for RNA-seq were prepared using Ion Ampliseq Transcriptome Mouse Gene Expression Kit following manufacturer's protocols.