scRNA-seq of AML: bone marrow patient 12
scRNAseq library preparation was performed on the ex vivo patient bone marrow samples using the Chromium Single Cell 3 Gene Expression v3 reagent kit (10x Genomics). The gel beads in emulsion (GEM) generation, cDNA amplification and library preparation were performed according to the manufacturers instructions. The prepared libraries were then sequenced on the Illumina NovaSeq 6000 instrument (Illumina) with paired-end reads 28 bp and 90 bp