DNA-Seq of citrullus lanatus: pooled leaves
Total genomic DNA was extracted using the Dot-Blot method. To enrich circular DNA, a rolling Circle Amplification (RCA) was performed using the TempliPhi 100 Amplification Kit (Cytiva 25-6400-10, Sigma Aldrich, St. Louis, MO, USA). The resulting RCA products were purified using the DNA Clean & Concentrator Kit (Zymo Research, Irvine, CA, USA), quantified with a NanoDrop spectrophotometer (Thermo Scientific, Wilmington, DE, USA), and submitted to a specialized sequencing company (MicrobesNG, Birmingham, UK) for long-read sequencing on the Oxford Nanopore Technology (ONT) MinION platform.