RNA-seq of mus musculus:sample5
Total RNA  from FVB mouse BMMs was extracted using Trizol reagent following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit, high-quality RNA samples with RIN number > 7.0 were used to construct sequencing library. Poly(A)+ mRNA was enriched using oligo(dT) beads, fragmented (Mg2+, 94C), and converted to strand-specific cDNA (dUTP method). PE150 libraries were sequenced on Illumina NovaSeq 6004.