GSM8615654: U2OS WT, S-trityl-L-cysteine (STLC), 16 h; Homo sapiens; OTHER
Cytoplasmic RNA from ribosome protected fragments (RPFs) were isolated from cytosolic cell lysate. Isolates were RNaseI-treated  and monosomes were purified using TRIzol reagent and chloroform. Libraries were constructed according to McGlincy NJ and Ingolia NT.  Methods. 2017