GSM8524660: Tgfβr2 KO, replicates 1 and 2, scRNAseq; Mus musculus; RNA-Seq
Retinas from P0 mice were isolated and dissociated using papain (Sigma-Aldrich, P3125) for 30 min. After mixing with Lo-ovomucoid buffer (1.5% BSA, 1.5% trypsin inhibitor dissolved in DPBS, pH 7.4), retinas were centrifuged at 400xg for 5 min. Cell pellets were resuspended in PBS containing 0.04% bovine serum albumin and filtered through a 40μm cell strainer (Falcon, 352340) to remove cell clumps. Cell pellets were labeled with Cell Multiplexing Oligo (10X Genomics). 10,000 cells were then input into the 10X library construction. GEM generation, reverse transcription, cDNA amplification, and library construction steps were performed according to manufacturer's instructions (Chromium Single Cell 3′v1/v2/v3 platform, 10X Genomics). Libraries were sequenced using a NovaSeq 6000 sequencer (Illumina) with paired-end 150 bp reads. Library construction steps were performed according to manufacturer's instructions (Chromium Single Cell 3'v1/v2/v3 platform (10X Genomics, Pleasanton, CA).