Oxford Nanopore amplicon sequencing of Lolium perenne: FT3, FTL9, pVRN1, pVRN2A, VRN2B genes
Target amplicons were generated by PCR from genomic DNA of individual 1 from F2 A24983 population. PCR products were barcoded using Oxford Nanopore native barcode NB01, multiplexed on R9.4.1 MinION flow cells, and sequenced. Reads were basecalled and demultiplexed using Guppy v6 prior to submission