Lactiplantibacillus plantarum strain BLP3
PacBio whole-genome sequencing library construction (Microbes)A sample of High-quality and high-molecular-weight DNA is required to prepare size- selected approximately 7-12 kb SMRTbell templates. We used PicoGreen to measure the concentration of genomic DNA. All samples passed screening QC criteria.For PacBio Revio sequencing, 3 ug of input genomic DNA was used for library preparation. For all size QC, We used the Femto Pulse System (Agilent) to determine the actual size distribution.The library insert sizes were in the optimal size range, we sheared gDNA was sheared with the Megaruptor 3 (Diagenode) and purified using AMPure PB magnetic beads (Pacific Biosciences) for size-selection.Total 10uL library was prepared using PacBio SMRTbell prep kit 3.0. SMRTbell templates were annealed using Revio SPRQ polymerase kit. Revio SPRQ sequencing plate and Revio SMRT Cell tray was used for sequencing. SMRT cells (Pacific Biosciences) using 24hr movie time were captured for each SMRT cell using the PacBio Revio (Pacific Biosciences) sequencing platform by Macrogen (Seoul, Korea). The subsequent steps are based on the PacBio Sample Net-Shared Protocol, which is available at https://www.pacb.com/