GSM8645791: Jurkat cells, Tat101, biological replicate 3; Homo sapiens; RNA-Seq
Total RNA was isolated with the miRNeasy kit (Qiagen). Libraries for RNA-Seq were synthesized with the TruSeq Stranded Total RNA library prep kit with the Ribo-Zero Human (Illumina) from 1 µg total RNA following the standard protocol. The libraries were pooled (n=12) and sequenced with a single NextSeq 500/550 High output kit v2.5 for 75 cycles (Illumina) in a NextSeq 500 sequencer (Illumina)