Genome sequencing of Solobacterium moorei 10714-02
S. moorei 10714-02 was isolated and cultured in Gifu Anaerobic Medium (GAM) at 37C in an anaerobic environment for 24 hours, confirmed by 16S rRNA sequencing, and stored as a glycerol stock. The samples used in this study was obtained under conditions of informed consent and with approval of the institutional review boards of each participating institute (National Cancer Center, 2013244; Tokyo Institute of Technology, 2014018). The genomic DNA was extracted using the NucleoBond high-molecular-weight DNA kit (Macherey-Nagel) according to the manufacturers protocol. The sequencing library for long reads was prepared using the Rapid Barcoding Sequencing Kit (SQK-RBK114) (Oxford Nanopore Technologies) and sequenced using an R10.4.1 Flowcell (FLO-MIN114) on a MinION Mk1B device with MinKNOW v.24.11.8 (Oxford Nanopore Technologies). Base calling and demultiplexing was performed using Dorado v.0.9.0 in Super Accurate Mode.