RNA-seq of B.rapa OLT: mock, rep2
Total RNA was obtained from floret frozen powder (100mg) using RNeasy Plant Mini Kit (Qiagen), and DNA contamination was removed using the DNA-free kit (Invitrogen, AM1906) according to the manufacturer's protocol. RNA was checked for quality and integrity (RIN>8; BioAnalyzer 2100, Agilent Technologies). The cDNA libraries were synthesized by the Universal Plus mRNA-Seq kit (Tecan Genomics, Redwood City, CA) and sequenced in paired-end 150 bp mode on NovaSeq 6000 (Illumina, San Diego, CA).