GSM8524341: H33WT-CBFA2T2KO-H3K4me3-ChIP; Mus musculus; ChIP-Seq
Cells were crosslinked with 1% formaldehyde for 10 min at room temperature and stopped with 125 mM glycine. The cell pellets were lysed  in a lysis buffer containing 50 mM HEPES pH7.5, 500 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% Na-deoxycholate, 0.05% SDS, 1 mM PMSF, 1 mM DTT and protease inhibitor cocktail. The lysate was sonicated to achieve an average DNA fragment length of 300-500 bp (Qsconica). ChIP-seq libraries were prepared using VAHTS Universal DNA Library Prep Kit for Illumina® V3 Kits (Vazyme.ND607).