GSM9515446: Untreated mt-DsRed2 hiNSCs 3D Culture, Control 3; Homo sapiens; RNA-Seq
RNA was harvested using RNeasy Kit (Qiagen, Catalog No. 74106). RNA samples were quantified and checked for integrity. Sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina following the manufacturer's instructions. mRNAs were enriched, fragmented, and synthesized into first and second-strand cDNA. Adapters were ligated and libraries were enriched by PCR.