GSM9037528: B3; Mus musculus; RNA-Seq
A total amount of 3 μg RNA per sample was used as input material for the RNA sample preparations. 4T1 cells transcriptomics were analyzed by Illumina NovaSeq 6000 for high-throughput deep sequencing. The mRNA libraries were constructed and sequenced. In brief, mRNA was purified with poly-T oligo beads and fragmented. First-strand cDNA was synthesized by random hexamer primers, and followed by second-strand synthesis. cDNA products were end-repaired and A-tailed before size selection and adapter ligation. The AMPure XP system was preferentially selected cDNA fragments between 150 and 200 bp in length. Individual sequence libraries were amplified by PCR. Libraries were pooled, and 150 basepair paired-end reads were sequenced. the mRNA libraries were prepared for sequencing using standard Illumina protocols