GSM8524916: Hepa 1-6 cell, Control, rep 1; Mus musculus; RNA-Seq
RNA was harvested using AG RNAex Pro Reagent (Accurate Biology). 2ug of total RNA was used for the construction of sequencing libraries. RNA library for RNA-seq were prepared using Dynabeads Oligo (dT) (Thermo Fisher, CA, USA), divalent cations (Magnesium RNA Fragmentation Module (NEB, cat.e6150, USA), SuperScript™II Reverse Transcriptase (Invitrogen, cat. 1896649, USA), E. coli DNA polymerase I (NEB, cat.m0209, USA) and RNase H (NEB, cat.m0297, USA), dUTP Solution (Thermo Fisher, cat. R0133, CA, USA) and UDG enzyme (NEB, cat.m0280, MA, US) following the manufacturer's protocols.