GSM8977191: caSTAT3, donor 3 (caS3_D3); Homo sapiens; RNA-Seq
The CD8+ CAR-T cell population was isolated using a FACSAria III cell sorter (BD Biosciences). Total RNA was then extracted from the purified CD8+ T cells using the NucleoSpin RNA Kit (Macherey-Nagel), according to the manufacturer's instructions. RNA libraries were prepared for sequencing using the TruSeq Standard mRNA Library Prep kit according to standard Illumina protocols.