GSM9511212: leaf, Col-0 wild type, rep 4; Arabidopsis thaliana; RNA-Seq
Total RNA was prepared from approximately 50 mg of leaf tissue from by using the E.Z.N.A. Plant RNA Kit (Omega BioTek) and DNA contamination was removed by using the TURBO DNA-Free kit (Invitrogen). Samples were diluted  to 100 μL of 5 ng/μL and submitted for short-read library preparation and AVITI sequencing. Libraries were prepared using the Watchmaker Genomics mRNA Library Preparation Kit with IDT xGen Unique Dual Index adapters following manufacturer's recommendations. Completed libraries were QC'd and quantified using a combination of Biotium AccuGreen High Sensitivity dsDNA and Agilent 4200 TapeStation HS DNA1000 assays. All libraries were normalized to a constant molar concentration prior to pooling. Libraries were pooled in equimolar amounts and the pool quantified using the Invitrogen Collibri Quantification qPCR kit. The shared pool was sequenced using an AVITI Cloudbreak Freestyle 2x150bp High Output Kit.