GSM8880342: Proliferating iMOPs_CTCF_CUT&Tag; Mus musculus; OTHER
For each CUT&Tag library preparation, 500,000 cells were used. Cells were incubated with primary antibodies targeting CHD7, CTCF, H3K4me3, and EP300, whereas the control sample was not treated with a primary antibody. DNA libraries were amplified via PCR and purified using SPRI beads. Sequencing was performed on the Illumina HiSeq 3000 platform with a 2×150 bp read configuration. CUT&Tag-IT Assay kit from Active Motif (cat# 53160).