GSM9006080: 2196 DE213 [scRNA-seq]; Mustela putorius furo; RNA-Seq
Cells were isolated from the collected tissues using a MACS Epidermis Dissociation Kit (Human) (Milltenyi) and a GentleMACS dissociator according to the manufacturer's instructions. Dead cells were removed using the MACS Dead Cell Removal Kit (Milltenyi) according to the manufacturer's instructions. Cells were counted and adjusted to achieve a targeted cell recovery of 10,000. Cells were loaded into gel-in-emulsion (GEM) beads using a 10X Chromium Controller (10X Genomics, 1000204) and the Single Cell 3' GEM, Library & Gel Bead Kit v3 kit (10X Genomics, 1000075). After GEM loading, RNA was reverse transcribed, incorporating cell-specific oligonucleotide tags. GEMS were then disrupted and cDNA was amplified and used to create a cDNA library using library construction kits (10X Genomics, 1000075). cDNA libraries were then sequenced on an Illumina HiSeq sequencer with 100 bp length in the 5' configuration. Library quality was assessed using the 2100 Bioanalyzer with the high sensitivity DNA kit (Agilent Technologies).