Illumina paired-end sequencing of Dendrophthoe pentandra leaf from Diospyros kaki host
Genomic DNA was extracted from mature leaf tissue of Dendrophthoe pentandra parasitizing Diospyros kaki. The DNA was enzymatically fragmented to obtain the desired insert size, followed by ligation with Illumina-compatible adapters. Libraries were amplified by PCR to incorporate full-length adapters with unique index sequences. Library quality and quantity were assessed using Qubit Fluorometer, TapeStation, and qPCR. Sequencing was performed on an Illumina NextSeq 2000 platform with 150 bp paired-end reads (300 cycles). FASTQ files were generated using Dragen BCL Convert v3.10.12 on the instrument.