GSM8585940: Neural cells, control, 3; Mus musculus; RNA-Seq
Treated and control cells were trypsinized, transferred to 1.5 mL Eppendorf-type tubes, and centrifuged at 500xg for 7 minutes. Obtained supernatants were instantly removed, and pelleted cells were snap-frozen at −80 °C pending RNA isolation. TRI Reagent™ Solution (ThermoFisher Scientific, USA) was used to purify total RNA according to a standard procedure. RNA was assessed for quality using the TapeStation 4150 System (Agilent, USA). RNA quantification was performed using the Qubit RNA BR assay (ThermoFisher Scientific, USA). Library was prepared from 50 ng of total RNA with the QuantSeq 3' mRNA-Seq Library Prep Kit FWD (Lexogen, Austria)