GSM8702929: control_replica10; Homo sapiens; RNA-Seq
RNA was extracted from FFPE tissue following the protocol of RecoverAll™ Total Nucleic Acid Isolation Kit (Invitrogen, Thermo Fisher Scientific). After nucleic acid extraction, TURBO DNA-free™ kit (Invitrogen, Thermo Fisher Scientific) was used to ensure genomic DNA removal from RNA samples. Whole transcriptome sequencing was done using the AmpliSeq Whole Transcriptome on the S5 System (Thermo (Fisher) Scientific, MA, USA). The targeted RNA-seq library was prepared using the Ion AmpliSeq Transcriptome Human Gene Expression Kit (Thermo (Fisher) Scientific, MA, USA). After that, the library was purified using Agencourt AMPure XP Beads (Beckman Coulter, Indianapolis, IN, USA) and quantified using an Ion Library TaqMan™ Quantitation Kit (Applied Biosystems, Waltham, MA, USA). After diluting the libraries to 100pM, they were pooled equally. The resulting template libraries were then sequenced on the Ion S5 XL Semiconductor sequencer using the Ion 540 Chip (Life Technologies Corporation, Carlsbad, CA) prepared on a fully automated Ion Chef System (Thermo (Fisher) Scientific, MA, USA).