16S-rRNA gene ASVs of growing media/growing media component
Microbial community analysis of growing media with different compost ratios and fertilization treatments. Samples were collected at multiple time points, freeze-dried, and ground before DNA extraction using a CTAB-based protocol. Bacterial 16S rRNA genes were amplified using the 341F/785R primers and sequenced on an Illumina MiSeq platform (2 300 bp, V3 chemistry). Data were processed using DADA2 for quality filtering and ASV inference, with taxonomic classification against the SILVA SSU database (release 132).