siRNA generation in reponsse to exogenous dsRNA application in Paramecium
siRNA generation in reponsse to exogenous dsRNA application in Paramecium
Cells were separated by centrifugation RNA was isolated via Trizol If not otherwise stated the NEB Next small libraray Kit was used for small RNA libraray creation, except the sample \"5allP_TS\" which used the Diagenode D-plex Kit based on template switch assisted cDNA generation.