GSM9626885: CB46894 treatment A; Homo sapiens; RNA-Seq
UCB-CD34+ were cultured respectively in Stemspan-AOF media supplemented in cytokine cocktails with and without C7 respectively as described previously for 3, 7 and 11 days. Harvested cells were then resuspended into single-cell suspensions into 1000-6000 cells/ul to be loaded into Chromium microfluidic chips with targeted cell recovery of 10,000 using the Chromium Controller (10x Genomics, USA). Single-cell RNA-seq libraries were prepared according to the manufacturer's instructions using the Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (Dual Index) (10 X Genomics, USA; PN-1000268). Captured libraries were sequenced on the Novaseq 6000 sequencer analyzer at PE150 and sequencing depth of 20,000 read pairs per cell (Novogene, China).