16S_DNA_Gut_Mix
Platform: MiSeq; Paired  end: Yes; Read length (bp) : 250; Primer pair: V4: 515F/806R; DNA polymerase : Phusion Hot Start II High Fidelity DNA Polymerase ;PCR cycle No. : 30; Adapters:Nextera XT;Sequencing depth  (avg reads per sample): 1209969; Library prep and sequencing protocol: V3 MiSeq reagent kit (600 cycles)