GSM8880016: sample02-2189; Rattus norvegicus; RNA-Seq
RNA was extracted from the pooled fetal testes homogenates (all remaining testes per litter minus three for testosterone measurements) and analyzed for changes in gene expression across all dose groups.  RNA was isolated and purified using Qiagen RNeasy Mini Kit with DNAse digestion according to manufacturer instructions (Valencia, California). RNA concentration and purity were assessed by NanoDrop 2000 Spectrophotometer (Thermo Scientific, Wilmington, Delaware). Aliquots of testes RNA from Gray et al. 2021 were then submitted to BioSpyder for Templated Oligo-Sequencing (TempO-Seq, BioSpyder Technologies, Carlsbad, CA, USA) using the rat whole transcriptome assay, which targets 21,119 protein coding genes using detector oligo probes. A full description of the assay can be found in Yeakley et al. (2017). Libraries were sequenced on an Illumina Hi-Seq 2500 (San Diego, CA, USA).