RNA-seq of cerebellum, kidney, liver, lung, muscle, spinal cord, and spleen from Eif2b1[N208Y] mice with or without 2BAct treatment
RNA-seq of cerebellum, kidney, liver, lung, muscle, spinal cord, and spleen from Eif2b1[N208Y] mice with or without 2BAct treatment
Mice were euthanized with CO2, and cerebellum, spinal cord, liver, kidney, lung, muscle and spleen were freshly collected and snap frozen in liquid nitrogen and stored at -80 â„ƒ 2BAct-maintained group: breeders were set up on 2BAct diet, experimental group maintained on 2BAct diet from birth into adulthood. 2BAct-withdrawal group: breeders were set up on 2BAct diet, experimental group maintained on 2BAct diet from birth until 4 months of age, then switched to control diet (0 mg/kg 2BAct) for 3 days prior to tissue collection. 2BAct-naive group: breeders set up on standard chow, experimental animals maintained on standard chow from birth to adulthood Tissues were treated with RNA-later ICE (Invitrogen #AM7030) for 24 hr at -20C and RNA extraction was performed following the MaxMAX total RNA isolation kit (Invitrogen #AM1830) RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA)