GSM8667561: MN07_LacZ_Rep1_hiPS6_H3K9me3_L1_CRISPRi; Homo sapiens; OTHER
CUT&RUN experiments were done following the protocol described in Skene&Henikoff  2018 (Skene & Henikoff, 2017). 300.000 cells per condition per antibody were used to do the analysis. For H3K4me3 CUT&RUN, standard CUT&RUN protocol was used. For the dCas9 CUT&RUN, a light crosslinking step was added. For CUT&RUN analysis Illumina sequencing libraries were prepared using the Hyperprep kit (KAPA) with unique dual indexed adapters (KAPA). CUT&RUN libraries were sequenced on a Nextseq500 instrument (paired end, 2x75bp).