GSM8957177: BM-MMC replicat 3; Mus musculus; RNA-Seq
total RNA was extracted using TRIzol (Invitrogen) and purified on a RNeasy column (RNeasy Mini Kit Qiagen 79254) Libraries were prepared using 418-500 ng of high-quality total RNA. Illumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus kit (Illumina) and IDT for Illumina DNA/RNA UD Indexes (Illumina) are used according to the manufacturer protocol. Size selection was performed using SPRIselect beads (Beckman Coulter). Library size and quality were confirmed on HS NGS Fragment kit for Fragment Analyzer (Agilent). Libraries were quantified by qPCR using the KAPA Library quantification kit for Illumina platforms (Roche Sequencing Solution). Samples were pooled in equimolar fashion.