16s cDNA-seq of human supragingival plaque
RNA extraction with Qiagen AllPrep DNA/RNA/miRNA Universal kit, purification with Zymo RNA Clean & Concentrator kit, DNA depletion with TURBO DNA-free kit, cDNA generation with iScript Select cDNA Synthesis kit w/ random primers, library preparation with V3-V4 amplification w/ gene-specific primers