HiSeq X Ten single Sequencing  (KAS24117033) (KAE24092738)
Whole bodies of 2.5 days postfertilization (dpf) embryos homogenized in Trizol solution were for sequencing. Raw data were obtained from Ebiogen company. The process is summarized briefly as follows: (1) RNA QC (quality check using Bioanalyzer 2100 system); (2) cDNA library (NEBNext UltraII Directional RNA-Seq kit); (3) sequencing (Illumina HiSeq X10 paired-end 100, 4G read) (4) Trimming (Average Q20 using BBDuk); (5) mapping (Mapping using TopHat to reference genome Calculation FPKM using Cufflinks); (6) normalization (Quantile normalization using EdgeR); (7) comparison (DEG master file production using ExDEGA); (8) repor