RNA-Seq of mus musculus: adult heart ventricle
RNA was extracted from snap frozen heart ventricles of 10-week old TRE-Plk1(T210D)-T2A-Ect2; MHC-rtTA mice treated with 0.1g/L doxycycline (n=3) from 9-10 weeks of age versus controls hearts on normal drinking water (n=4) with a RNeasy Mini Kit (QIAGEN, 74106). RNA samples with RIN scores >8 were processed to generate Stranded mRNA-Seq libraries (KAPA mRNA HyperPre Kit, Roche, KK8581). An Illumina NextSeq 500 system was used to perform sequencing (75-bp, high-output sequencing kit for single-end sequencing).