GSM9039118: MCF10A_H1047R_3; Homo sapiens; RNA-Seq
Two million cells were seeded in 10 mm Petri dishes two days prior to RNA extraction. Total RNA was extracted using the RNeasy Kit for RNA Purification (Qiagen) following the manufacturer's protocol. RNA quality was assessed using the Agilent TapeStation system, which provides RNA Integrity Number (RIN) scores to evaluate sample integrity. Only RNA samples with RIN ≥ 7 were used for downstream RNA sequencing to ensure high-quality transcriptomic data. RNA concentration was measured using the NanoDrop spectrophotometer, ensuring adequate yield for library preparation. Only samples with A260/A280 ratios between 1.8 and 2.1 were used to maintain RNA purity. RNA-seq libraries were prepared using the NEBNextUltra II Directional RNA Library Prep Kit for Illumina with 1000 ng of input material. Sequencing was performed on the NovaSeq X Plus platform to generate high-depth transcriptomic data.