GSM9359816: Chicken SPs, St 24, 2; Gallus gallus; RNA-Seq
After UV irradiation, total tissue lysate was collected and purified. 2nd strand DNA was synthesized using Rnase H, DNA pol I, and DNA ligase mix. Only cDNAs from UV-irraidated region are filled-in to 5' end of T7 promotoer, being linearly amplified as RNAs via IVT reaction. RNA libraries were then reverse-transcribed and sequenced on Illumina NovaSeq 6000 (R1: UMI and barcode, R2: cDNA).