B1
Comprehensive expression profiling of mRNAs and non-coding RNAs (lncRNAs, circRNAs, and miRNAs) was achieved using high-throughput sequencing on the Illumina NovaSeq 6003 platform; first-strand cDNA was synthesized using random primers, followed by RNase H digestion of the RNA template. Second-strand cDNA was generated using dNTPs and subsequently purified. After end-repair, A-tailing, and adaptor ligation, the cDNA fragments were amplified by PCR to generate the final sequencing libraries.