Exercise-associated changes of leukocyte gene expression in Stratin-associated myopathy - A case study
Exercise-associated changes of leukocyte gene expression in Stratin-associated myopathy - A case study
The blood was collected from the individual for 3 visits and timepoints, PBMC were isolated according to the manufacture's protocol  RNA were isolated from the PBMC using RNeasy Mini Kit (Qiagen, Valencia, CA). The quality of the total RNA assessed using NanoDrop® ND-8000 spectrophotometer (NanoDrop Technologies, Wilmington, DE) and Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA) . All RNA samples should display a concentration above 50 ng/µl, purity - 260/280 ~ 2.0., integrity - RIN > 8.0 and average > 8.5 The library prepared with Illumina TruSeq RNA kit according to the manufacturer's instructions (Illumina, San Diego, CA). Additionally, the KAPA RNA HyperPrep Kit with RiboErase (HMR) (Roche, Pleasanton, CA) will be used in order to provide improved coverage of precursor mRNAs and noncoding RNA