P5_H3
Ribosomal RNA (rRNA) depletion was performed using the QIAseq FastSelect H/M/R and globin modules, starting with 300ng of total RNA per sample as input material. Fragmentation time was 7 or 15 min, considering their RNA integrity to obtain a mean insert size of ~275-325bp according to the kits manufacturer protocol. Ribodepleted RNA was then used as input material for Illumina's NEBNext Ultra II RNA library prep kit.