RNA-seq of mouse hearts infused with Angiotensin II
RNA-seq of mouse hearts infused with Angiotensin II
The libraries were prepared for 150bp paired-end sequencing using TruSeq Stranded Total RNA Sample Prep Kit with Ribo-Zero H/M/R.  mRNA molecules were purified and fragmented from 2Î¼g of total RNA using oligo (dT) magnetic beads.  The fragmented mRNAs were synthesized as single-stranded cDNAs through random hexamer priming. By applying this as a template for second strand synthesis, double-stranded cDNA was prepared. After sequential process of end repair, A-tailing and adapter ligation, cDNA libraries were amplified with PCR (Polymerase Chain Reaction). Quality of these cDNA libraries was evaluated with the Agilent 2100 BioAnalyzer (Agilent, CA, USA). performed by Bioneer in south korea. 