Sequencing libraries were prepared using the Illumina DNA PCR-Free Library Prep (Illumina, USA). Around 500-1000ng of genomic DNA was used from each sample for an initial DNA tagmentation using Bead-Linked Transposomes (BLT-PF) which fragment and tag the DNA with adapter sequences. Following index ligation, the final libraries were then purified by a double-sided bead purification procedure and quantified to a final loading concentation of 2 nM by Qubit ssDNA Assay Kit (Thermo Fisher Scientific, USA). Libraries from each family (Trios) were pooled and then sequenced using paired end mode (2X150bp) on an S1 flow cell and the NovaSeq system (Illumina, USA) to generate, on average, 182 GB of data and an autosomal coverage of >30X