Fecal functional metagenomics of colon cancer and healthy fecal DNA samples
The libraries were prepared from the samples by Twist NGS LibraryPreparation Kitsfor Illumina (CAT No. /ID 104119). The amplified libraries were analyzed on TapeStation 4150 (Agilent Technologies) usingHigh Sensitivity D1000 ScreenTape as per manufacturers instructions. After obtaining the Qubit concentration for the library and the mean peak size from Tape Station profile, library will be loaded onto illumina Novaseq 6000 for clustergeneration and sequencing. Before initiating denovo assembly, data was filtered to remove sequencing adapters and low quality sequences below QV20. Assembly of the clean reads was carried out using MEGAHIT v1.2.9 which is a standalone metagenome assembly program for assembling large and complex metagenomics data.