GSM8667659: CV1_8_CecalBac-RNA; Mus musculus; RNA-Seq
RNA was extracted from cecal contents using Zymo Tri-Reagent and Qiagen Power Fecal Pro RNA isolation kits followed by further purification using Zymo RNA Clean and Concentrate kits. Qiagen QIAseq FastSelect 5S/16S/23S kit was used to deplete rRNA from the samples prior to library preparation. Libraries were prepared using the Roche KAPA RNA HyperPrep Kit with KAPA UDI primers following manufacturer's recommendations. Completed libraries were QC'd and quantified using a combination of Qubit dsDNA HS and Agilent 4200 TapeStation HS DNA1000 assays. The libraries were pooled in equimolar proportions and the pool quantified using the Invitrogen Collibri Quantification qPCR kit.