Rhizosphere soil microbiome of Newhall navel orange
Genomic DNA was extracted from rhizosphere soil using the MoBio PowerSoil DNA Isolation Kit. The V4 hypervariable region of the bacterial 16S rRNA gene was amplified with barcoded primers 515F/806R and Phusion High-Fidelity Master Mix. Libraries were constructed from purified amplicons and SINGLE-end sequenced (2x250 bp) on an Illumina MiSeq platform. The submitted data represent the final effective tags: raw SINGLE-end reads were demultiplexed, merged (FLASH v1.2.11), quality-filtered (fastp v0.23.1), and chimera-checked (VSEARCH v2.16.0 against SILVA v138).