RNA-seq of mus musculus: C57BL6 bone ostroclast
Following pretreatment, RNA samples were extracted from bone marrow mononuclear cells (BMMCs) using standard protocols. Quality control of the RNA samples was performed using a Qsep 400 bioanalyzer. Total RNA was quantified using a Qubit 2.0 Fluorometer. RNA library preparation was carried out using the Illumina TruSeq Stranded Total RNA Library Preparation Kit according to the manufacturers instructions. Specifically, 200 ng of total RNA was used for library preparation. Library preparation was quantified using a Qubit 2.0 Fluorometer. After successful library inspection, libraries were pooled based on their effective concentrations and required downstream data volumes for Illumina sequencing. Differential expression analysis between the two groups was performed using the DESeq2 package. Significant differences were identified based on a p-value threshold of <0.05 and a log2 fold change of >1.