Pampus argenteus  muscle
Genomic DNA was extracted from the samples and purified using the QIAamp DNA Mini Kit (Qiagen). DNA concentration was measured using the Qubit 4.0 Fluorometer (Thermo Fisher), and quality was assessed via 1% agarose gel electrophoresis. 500 ng of DNA was fragmented to 200-500 bp using the Covaris S220 ultrasonicator. End repair, A-tailing, and adapter ligation were performed using the NEBNext Ultra II DNA Library Prep Kit (NEB). Library amplification was carried out with 12 PCR cycles using Illumina universal primers. Library size distribution was checked using the Agilent 2208 Bioanalyzer, and concentration was quantified with Qubit.