RNA-Seq of tomato fruit
Using Oligo(dT) magnetic beads, mRNA with a polyA structure is enriched from the total RNA. RNA is fragmented into approximately 300 bp fragments using ion fragmentation. Using RNA as a template, the first strand of cDNA is synthesized with random hexamer primers and reverse transcriptase. The second strand of cDNA is then synthesized using the first strand of cDNA as a template.