polyA+ RNA-Seq of C57BL/6xCBA Mettl4 knockout mice
Mice line with Mettl4 gene knockout was created by microinjection of Cas9 mRNA along with sgRNA targeting Mettl4 gene into C57BL/6xCBA mice zygotes. Mettl4+/- mice were back-crossed to the C57BL/6 genetic background and mated to obtain homozygous viable Mettl4-/- mice. Total RNA from the mouse liver samples was extracted using QIAzol lysis reagent. NEB NEBNext Ultra II RNA Library Prep Kit for Illumina (E7770) was used for library preparation.