GSM8773456: CH1 - Control Hypoxia; Homo sapiens; RNA-Seq
Cells were washed in PBS, lysed using the RNAeasy kit QIAzol lysis agent, scraped, and homogenised using needles before being flash-frozen in liquid nitrogen. RNA was extracted from the lysates using the Qiagen RNeasy Mini kit following the manufacturer's protocol. Stranded paired 150-bp poly-A enrichment protocol by Novogene