GSM9037534: SUP-B15_DMSO_H3K27ac_rep2; Homo sapiens; ChIP-Seq
ChIP-Seq for H3K27ac was done as described in Boulianne et al using anti-Histone H3 (acetyl K27) antibody – ChIP Grade (ab4729) (Abcam) and polyclonal rabbit IgG (isotype control) (sc2027) (Santa Cruz Biotechnologies), with the exception that initial cross-linking was performed at RT and 1mio cells were used for crosslinking instead of 15mio. ChIP-Seq libraries were generated from ChIP immunoprecipitated DNA using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina (NEB) and sequenced either at Novogene or at the in-house sequencing facility of the MRC London Institute of Medical Sciences at Imperial College London.