GSM9723342: SH-SY5Y, WT, 1-deoxysphinganine treated, rep3; Homo sapiens; RNA-Seq
Total RNA was extracted using the miRNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. Following treatment, cells were washed with PBS and lysed in 700 µL QIAzol reagent. Lysates were homogenized using QIAshredder columns (Qiagen), and RNA was purified using the miRNeasy Mini Kit. RNA concentration and purity were assessed using a NanoDrop spectrophotometer. RNA-seq