RNAseq of mononuclear cells derived from peripheral blood or bone marrow of aute promyelocytic leukemia patients
RNAseq of mononuclear cells derived from peripheral blood or bone marrow of aute promyelocytic leukemia patients
Mononuclear cells were isolated from peripheral blood or bone marrow of APL patients using density gradient centrifugation. Diagnosis of APL was based on revised International Working Group criteria. Isolation of RNA from APL patients' cells was performed with the Nucleo Spin RNA Kit (Macherey-Nagel) according to the manufacturer's instructions. RNA concentration and integrity were evaluated on an Experion (Bio-Rad). Sequencing libraries were synthesized by TruSeq Stranded Total RNA Kit (Ribo-Zero Gold) (Illumina) per the manufacturer's instructions.