RGc8
Total RNA was isolated from flow-sorted bone marrow of live DC precursors (Lin-Sca-c-kit+/-CXCR1/Flt3/Csf-1R+/-) using RNAeasy Plus Universal (Qiagen). Dual-indexed libraries were generated using Takara/ Clontech stranded total RNA-seq kit v2-pico input mammalian reagents (Takara)