GSM8666777: 120hpf_sibCTL_replicate_2; Danio rerio; RNA-Seq
Whole sibCTL and tet2-/-tet3-/- eyes were extracted from zebrafish embryos and larvae and were dissociated using either papain or trypsin, with genotype and timepoint-specific protocols utilized Library was generated using manaufacturer's instructions (scRNAseq v2 3' Paired-End). For each experiment, in 2 separate wells, sibCTL and  tet2-/-tet3-/-  dissociated cells were resuspended in 10x mastermix and loaded with gel beads and partitioning oil to generate GEMS.  Each GEM contained sufficient priming material to enable cDNA amplification of poly-adenylated mRNA molecules with cell-specific(10x barcode) and transcript-specific(UMI) tags. Pooled cDNA was then cleaned up with silane beads, and PCR amplified. After PCR amplification, cDNAs were size-selected using SPRIselect reagent. During library construction P5 and P7 ends were added for sequencing.