GSM8664588: Platelet_iPAH4; Homo sapiens; RNA-Seq
Total RNA was extracted from platelets of idiopathic pulmonary arterial hypertension (iPAH) patients and healthy controls using Trizol Reagent (15596-026, Invitrogen, USA). Messenger RNA (mRNA) was enriched by depleting ribosomal RNA (rRNA) with the rRNA Depletion Kit (NEBNext, MA, USA). RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina and assessed with an Agilent 4200 Bioanalyzer