GSM8773189: Endoderm_BADC_RNA-seq_rep1; Mus musculus; RNA-Seq
mES cells and endoderm differentiated mES cells were collected in RLT plus buffer (Qiagen) and stored at −80°C until RNA extraction. RNeasy Plus Micro kit (Qiagen) with on-column DNase digestion was used for RNA extraction. RNA-seq library preparation with Poly-A selection was performed with 1μg of purified RNA using the TruSeq Stranded mRNA kit from Illumina. Library quality was assessed with a fragment analyzer before sequencing.