eDNA metabarcoding of vertebrates: seawater filtrate
Water eDNA samples were amplified using primers MiVert-D-F_ONT: 5-TTTCTGTTGGTGCTGATATTGCGGGYYGGTAAAWYTYGTGCCAGC-3 and MiVert-R_ONT: 5- ACTTGCCTGTCGCTCTATCTTCCATAGTGGGGTATCTAATCCCAGTTTG -3. Before pooling, amplicons were indexed using Rapid PCR Barcoding Kit 24 V14 (Oxford Nanopore Technologies). DNA library was prepared using Ligation Sequencing Kit V14 (Oxford Nanopore). Sequencing was performed using MinION sequencing device (Oxford Nanopore Technologies)