16S rRNA-seq of mus musculus: adult male feces (low fiber diet)
An index PCR was performed to attach dual barcodes and Illumina sequencing adapters using Nextera XT Index kit (Illumina). Final PCR products were verified on 1.5% DNA agarose gel and quantified using Pico dsDNA assay (Invitrogen). An equal molar of each sample was combined and purified again using Ampure XP beads as the library