GSM8644563: HEK293T,MED23-KD-2,4SU-labelled 15min, rep1; Homo sapiens; OTHER
The labelled cells were harvested immediately with TRIzol. Total RNA was extracted with phenol/chloroform/isoamyl alcohol. The 4SU-labelled RNAs were biotinylated and enriched using streptavidin beads. The 4SU RNAs were subjected to construct strand-specific DNA libraries using an NEBNext Ultra Directional RNA Library Prep Kit for Illumina.