GSM9360876: SAS Day 14 CLL B-cell monoculture replicate 1; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNeasy Mini Kit (Qiagen) following manufacturer instructions. RNA quality and quantity were assessed prior to library preparation. Stranded mRNA-seq libraries were prepared using the Illumina Stranded mRNA Prep kit (poly-A mRNA enrichment) with IDT for Illumina Unique Dual (UD) Indexes. Libraries were prepared following the manufacturer's recommended protocol, pooled, and sequenced on an Illumina NovaSeq 6000 S1 flow cell using a paired-end 2 × 75 bp configuration.