Kappa Chain BCR-seq of mustela putorius furo: PBMCs
cDNA isolation: RNA was isolated from bulk cell samples using the RNeasy Mini Kit (Qiagen, 74104), and cDNA was synthesized using SuperScript IV (Invitrogen, 18091050), both per the manufacturers instructions. Priming strategy: multiplexed forward primer set from Wong...Wheatley 2020 targeting IGKV leader2 sequences and single kappa-CH1-specific reverse primer. Sequencing: performed by Genomic Sequencing and Analysis Facility at UT Austin, Center for Biomedical Research Support (RRID# SCR_021713) using the Illumina MiSeq 2 x 300 paired-end platform (MiSeq Reagent Kit v3, 600 cycles, MS-102-3003).