Hyb-Seq of Carex species
Samples were sonicated in a Bioruptor Pico Ultrasonicator to reach a fragment size of 550 bp. Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Massachusetts, US) and posteriorly quality checked using a Qubit 2.0 Fluorometer. Once the samples were indexed, they were included in equimolar (500-700 ng) pools of approximately 15 samples. Enrichment was carried out with the Cyperaceae-specific baits kit and posterior hybridization was made following the myBaits kit protocol from Arbor Bioscience. Sequencing was externally carried out by Arbor Bioscience on an Illumina platform, model HiSeq NovaSeqX 150 PE