Potter Cove sediment slurry SIP incubation - 13C Acetate.Lepidocrocite at 5 degrees C - nucleic acid extract - Bacteria 16S rRNA gene
total nucleic acids were extracted from slurry with a phenol-chloroform protocol. PCRs were performed using primers with unique 8 bp barcodes at forward and reverse primers. Multiple samples were pooled into one library for sequencing. During demultiplexing reads were sorted by read direction forward-reverse or reverse-forward using the primer sequence, resulting in multiple paired fastq files (=runs) for the same ENA experiment