GSM9628981: RERUN__Enriched_Apheresis, GEM-X; Homo sapiens; RNA-Seq
Patient's PBMCs samples were thawed and washed with warm DMEM + 10% FBS followed by automated cell count. Cells were resuspended in 1X PBS + 1% BSA on a range of 100-2,000 cells/μl. The number of cells loaded on the 10X Genomics Single Cell Chromium Controller was adjusted according to differences in samples concentrations aiming to achieve the highest targeted cell recovery number. After the generation of the single cell gel beads (GEMs), captured cells were lysed, and barcoded full-length cDNA from poly-adenylated mRNA was produced and amplified. An additional amplification was performed with primers specific to TCR constant regions prior to TCR library construction. GEM-X and TCR libraries were prepared according to the manufacturer's protocols (10x Genomics Chromium GEM-X Single Cell V(D)J + 5′ Gene Expression (v3) kit).