16S rRNA amplicon of soil metagenome
Microbial genomic DNA was extracted from soil samples in duplicate using a GeneMATRIX Soil DNA Purification Kit (EURx, Gdask, Poland), according to the manufacturers recommendations. Microbial community structure was analyzed using the V4 region of the 16S rRNA gene amplicon, which were sequenced with MiSeq Illumina technology using universal primers 515F (5-GTGYCAGCMGCCGCGGTAA) and 806R (5-GGACTACNVGGGTWTCTAAT). Sequence libraries were generated using NEB Next Ultra II FS DNA PCR- free LibraryPrep Kit with an Illumina PE250 platform.