GSM9449467: Control rep3; Homo sapiens; RNA-Seq
Total RNA was extracted from HAECs exposed to EndMT-inducing conditions and GSK-126 (5 μM) using TRIzol® Reagent (Invitrogen, Australia) and the Direct-zol™ RNA Miniprep Kit (Zymo Research, USA), following the manufacturer's protocols To enrich for mRNA, 1 μg of total RNA was processed using the NEBNext® rRNA Depletion Kit (New England Biolabs, USA). Barcoded libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs)