scRNA-seq of a HIVBaL-infected MISTRG-6-15: HSPCs (mCD45hCD45+CD34+CD38CD45RA) from bone marrow
Bone marrow cells harvested from a HIVBaL-infected MISTRG-6-15 mouse at the age of 13 weeks, 2 weeks post-infection. HSPCs were sorted (mCD45hCD45+CD34+CD38CD45RA) using the BD FACSAria III Cell Sorter in PBS with 1% BSA. RNA was extracted using the Qiagen RNeasy Mini Kit. The RNA-Seq library was prepared using the Takara Clontech SMARTer PCR cDNA Synthesis Kit according to the manufacturers protocol. Library quantification and quality assessment were performed using a Qubit fluorometer (ThermoFisher Scientific) and a Tapestation (DNA High Sensitivity chip, Agilent Technologies). The library was sequenced on an Illumina NovaSeq 6000 using paired-end 2890 bp sequencing mode.