RNA-Seq of Aeromonas veronii
Extract total RNA to remove ribosomal RNA, purify and fragment RNA. Synthesize one strand of cDNA and use DNA polymerase I and RNase H to synthesize two strands of cDNA. Double stranded cDNA with A tail and connected to sequencing adapter. The final cDNA library was obtained by purifying and amplifying the connected product. The sequencing library was sequenced using the HiSeq sequencing platform.