GSM9588857: PC_004, Carcinoma, CDC73 mut; Homo sapiens; RNA-Seq
Parathyroid adenoma and carcinoma tissues were dissociated into single-cell suspensions using the Tumor Dissociation Kit, human (Miltenyi Biotec) and the gentleMACS Octo Dissociator with heaters (Miltenyi Biotec) using program 37C_h_TDK_1 for 30–45 min, according to the manufacturer's protocol. Cell suspensions were filtered through 100 μm strainers, centrifuged at 400 × g for 5 min at 4 °C, and resuspended in 0.04% bovine serum albumin (BSA) in PBS at an appropriate concentration for 10x Genomics loading. All libraries were sequenced using NovaSeq 6000 system (Illumina) in paired-end mode.