RNA-Seq of Opsariichthys evolans: brain
30 mg of tissue were dissected and homogenized with 600 l Trizol solution for RNA sequencing. RNA was extracted from the homogenized Trizol solution using RNeasy Plus Mini Kit (Cat: 74134, Qiagen), and quantified using the Agilent 2100 Bioanalyzer. A short-read sequencing library was constructed from approximately 1.5g of the quantified RNA using the TruSeq Stranded mRNA Library Prep Kit (Cat. No. 20020595). It was then sequenced on the Illumina NovaSeq 6000 through the services of GENOMICS.