Gill Transcriptome of Tor putitora
The paired-end sequencing library was prepared using NEB NextUltra RNA Library Prep Kit for Illumina (Cat. No. E7770) The library preparation process was initiated with 1000 ng input. The mRNA enrichment was done as per the user manual and mRNA was subjected to fragmentation, first & second-strand cDNA synthesis, end-repair, 3 adenylation, adapter ligation, and selective enrichment of adapter-ligated DNA fragments through PCR amplification. The library validation was performed on the Agilent 4150 tape station using HSD1000 ScreenTape as per the instructions provided by the manufacturer.