GSM8775519: Splenocytes, mRNA-LNP, intradermal; Rattus norvegicus; RNA-Seq
The splenocytes were obtained at 4 weeks of boost. CD45+ splenocytes were sorted by flow cytometry. The cells were then loaded into a 10× chromium microfluidic system based on the manufacturer's guidelines. Library was perfomred according to the manufacter's instruction. A set of libraries was obtained from 10× loaded samples: a 3 × gene expression messenger RNA library. The Illumina NovaSeq 6000 sequencing platform and PE150 sequencing mode were used to sequence the completed library; the sequencing amounts should be more than 20 k reads/cell.