GSM9511193: G1_mac2-Segment 1; Rattus norvegicus; OTHER
FFPE tissue sections were mounted on positively charged histology slides. FFPE tissue sections were subjected to in situ hybridization with GeoMX RNA Detection probe (WTA or CTA). Nuclear stain marker and antibodies of target protein were applied to each section. Slides were load on the GeoMX DSP. ROIs were selected and the GeoMX software was used to define each AOI in ROIs as one segment containing positive immonofluorescent signals. Once AOIs were defined, the DSP then exposed AOIs to 365nm light (UV), releasing the indexing oligonucleotides, which were collected with a microcapillary. The GeoMX libraries for NGS were prepared using GeoMX Library Prep Kit following maufacture's protocols.