metariboseq of skin microbiome
Sampling was performed by applying pressure to a 2 x 2 cm area of one side of the forehead in a circular motion for 60 seconds with sterile cotton swabs (Fisher #22-029-630) pre-moistened with a swabbing solution (Tris-EDTA (TE) buffer + 0.5% Tween-20 + 1% TritonX-100). We processed the swabs by extracting RNA and prepare the metariboseq libraries using methods described in our previous work https://pubmed.ncbi.nlm.nih.gov/37214910/