GSM9510537: GC-WT-69,ChMKO mice; Mus musculus; RNA-Seq
Total RNA was extracted using Trizol reagent kit (Invitrogen, Carlsbad, CA,USA) The enriched mRNA was fragmented into short fragments using fragmentation buffer and reversly transcribed into cDNA by using NEBNext Ultra RNA Library Prep Kit for Illumina(NEB #7530,New England Biolabs, Ipswich, MA, USA).