Whole genome sequence analysis of Streptococcus mutans from Dental Caries Patients
The DNA fragments undergo end repair where in the mix converts the overhangs resulting from fragmentation into blunt ends. The 3 to 5 exonuclease activity of end repair mix removes the 3 overhangs and polymerase activity fills in the 5 overhangs. To the blunt ended fragments adenylation is performed by adding single A nucleotide to the 3 ends. To the adenylated fragments loop adapters were ligated and cleaved with uracil-specific excision reagent (USER) enzyme. The samples are further purified using AMPure beads and The DNA is then enriched by PCR with 6 cycles using NEBNext Ultra II Q5 master mix, illumina universal primer and sample-specific octamer primers.