RNA-Seq of mus musculus: Acute isolated microglias from sham or SAH brain
RNA from sorted cell populations underwent reverse transcription followed by cDNA pre-amplification for quality control. QC-passed samples proceeded to formal amplification, with purified cDNA used for full-length library construction. Libraries passing quality assessment were sequenced on the Illumina NovaSeq 6000 platform (150 bp paired-end reads).