PIG927_T2
The V3-V4 region of the 16S rRNA gene (460 bp) was amplified, amplicons were produced using the universal primers Pro341F: 5-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNBGCASCAG-3 and Pro805R: 5GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACNVGGGTATCTAATCC-3 (Takahashi et al. 2014) using the Platinum Taq DNA Polymerase High Fidelity (Termo Fisher Scientific, Italy) and sequenced using the Illumina MisSeq platform 300x2bp. The libraries were prepared using the standard protocol for MiSeq Reagent Kit V3 and sequenced on MiSeq platform (Illumina Inc., San Diego, Ca, USA).