16S microbial soil metagenome from Vanjipura beach
The amplicon libraries were prepared from the QC passed DNA samples using Nextera XT Index Kit (Illumina Inc). Briefly, amplification of the 16S V3-V4 region specific primer set was carried out using the received metagenomic DNA samples. 3L of the PCR product was QC checked on 1.2% Agarose gel by running at 120V for 60 min.QC passed amplicons were indexed by amplifying with i5 and i7 primers that add multiplexing index as well as common adapters required for cluster generation(P5 and P7) as per standard Illumina protocol. The products were purified using AMPureXP beads.