GSM8615136: Wild-type [WGBS]; Mus musculus; Bisulfite-Seq
Genomic DNA was purified from cell lysates using Zymo Genomic DNA Clean & Concentrator-10 Kit. 100 ng of genomic DNA was bisulfite converted using Zymo EZ DNA Methylation-Lightning Kit according to the manufacture's protocol. This was followed by a second strand synthesis reaction and then addition of adapters through tagmentation using Illumina's Nextera® kit. PCR was performed with Illumina Nextera Unique Dual Indices. Library quality control was performed on the Agilent 2200 TapeStation. Libraries were sequenced on an Illumina NovaSeq 6000 instrument.