GSM8841686: Overexpressing rocR (low cdGMP), rep3; Acinetobacter baumannii AB0057; RNA-Seq
Cultures were harvested by centrifugation (or direct resuspension for desiccated cells) in RNAlater to preserve RNA integrity. RNA was isolated using athe Qiagen RNAeasy Kit with on-column DNAase digestion (Qiagen). RNA quality was assessed using a Bioanalyzer. Library preparation was performed using Illumina's Stranded Total RNA Prep Ligation with Ribo-Zero Plus kit and 10bp IDT for Illumina indices.