GSM8743964: Cas9 A2-CAR Treg, donor 3526; Homo sapiens; RNA-Seq
Cells were lysed 16-hrs post-stimulation using TRIzol® and total RNA was extracted with the PureLink RNA MicroKit (as per manufacturer's guidelines). RNA integrity was confirmed to be >8.0, then RNA was prepared using the Illumina® Stranded mRNA Prep kit.