GSM9193413: GBM12 cells, DMSO-3; Homo sapiens; RNA-Seq
RNA samples for sequencing were isolated using the Qiagen RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol. The library's quality and quantity were measured by a Bioanalyzer Nanochip. Libraries were prepared according to the manufacturer's instructions. Briefly, poly(A)+ RNA was fragmented and reverse transcribed to synthesize first-strand cDNA, followed by second-strand synthesis. After end-repair, A-tailing, and adapter ligation, the libraries were PCR amplified and purified. Library quality and quantity were assessed using the Bioanalyzer at the Columbia Genomics Center. Sequencing was performed on the Element AVITI platform using paired-end 2x150 bp reads.