GSM9514825: SN_Graft_2; Mus musculus; RNA-Seq
Total RNA from sciatic nerve tissue was collected afer 13 weeks in TRIzol Reagent (Invitrogen) and homogenized using poly-T oligo-attached magnetic beads. RNA was then isolated using the PureLink RNA Mini Kit (Invitrogen) followed by DNAse treatment (Thermo Fisher) to eliminate the presence of co-precipitated extracellular DNA. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dTTP for non strand specific library or dUTP for strand specific library. -- this part was done by Novogene