Amplicon 16S V3-V4 sequencing of dog gut microbiome
Sequencing of the 16S rRNA gene (V3V4 region) to a depth of 50,000 tags per sample was performed using the Illumina paired-end (2  250 bp) technology. The primer pair 341F (5-CCTAYGGGRBGCASCAG-3) and 806R (5-GGACTACNNGGGTATCTAAT-3) was used to amplify the V3V4 region.