GSM8525066: Human Primary glioblastoma-derived CD11b+ cells, patient 1; Homo sapiens; RNA-Seq
Human glioblastoma tumors were dissociated enzymaticly and mechanically. Red blood cells were lysed, and myelin and debris were removed with a density gadient reagent. CD11b+ cells were isolated using CD11b microbeads and magnetic activated cell sorting (MACS). CD11b+ cells were fixed and frozen, and right before use, cells were rehydrated. Cells were processed for scRNA-seq using the 10X Genomics Chromium Next GEM single-cell 3' reagent kits v3.1, following manufacturer's instructions (CG000204 Rev D).