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DNA was extracted from pure Salmonella isolates using the KingFisherTM Flex Purification System (Thermo Fisher Scientific) and eluted in MGW prior to quantification on a Qubit Fluorometer (Thermo Fisher Scientific). Ion Torrent libraries were prepared, amplified, and quantified as previously described (Jenkins et al., 2021 Pathogens 10(11):1367). The quantified libraries were diluted to a concentration of 100 pM and loaded onto an Ion 530 chip using the Ion Chef system. The prepared chip was then run on the Ion Torrent S5 next generation sequencing system.