Bulk RNA-seq of mouse testis (Wildtype, replicate 2)
Total RNA was extracted from mouse testis tissues using TRIzol reagent following the manufacturers protocol. RNA integrity and concentration were assessed using an Agilent 2100 Bioanalyzer and NanoDrop spectrophotometer. Samples with RNA integrity number (RIN)  7.0 were used for library construction. Poly(A)+ mRNA was enriched using oligo(dT) magnetic beads, followed by fragmentation, first- and second-strand cDNA synthesis. Sequencing libraries were prepared using a commercial RNA-seq library preparation kit according to the manufacturers instructions, including end repair, A-tailing, adapter ligation, and PCR amplification. The final libraries were quantified and quality-checked, pooled equimolarly, and sequenced on an Illumina platform to generate paired-end reads.