GSM9409633: Placenta, Str - let7f, rep6, female; Mus musculus; RNA-Seq
Placental tissues were homogenized in Trizol reagent (ThermoFisher, 15596026) followed by phase separation with chloroform. RNA isolation proceeded with the aqueous phase using the RNeasy Kit (Qiagen, 75144). Libraries of placental Poly-A selected mRNA were prepared with the TruSeq Stranded mRNA Library Prep (Illumina, 20020595)