WGS of mus musculus: adult tail clipping
Genomic DNA was extracted from tail clips using the Qiagen DNeasy Blood & Tissue Kit. Libraries for WGS were prepared from 500ng of high molecular weight genomic DNA using the KAPA Hyper Prep Kit (v5.16) (Kapa Biosystems, Wilmington, MA USA). In brief, DNA is sheared to an average size of 450 bp, then end repaired and A-tailed DNA fragments were ligated to uniquely barcoded dual indexes (IDT, Coralville, IA USA), after which minimal PCR (6 cycles) was performed. Whole-genome libraries were sequenced to a depth of 30x on Illumina NovaSeq X.