GSM8493839: 1dPI_3; Homo sapiens; ncRNA-Seq
Total RNA of NHBE cells uninfected or infected with SARS-CoV-2 VoCs for 1 hr, 24 hr and 72 hr was extracted using miRNeasy extraction kit (Qiagen) and according to the manual's instructions. Small RNA libraries were generated with the Lexogen small RNA-Seq Library Prep Kit (Lexogen GmbH, Vienna, Austria), based on 500 ng total RNA input Final libraries were multiplexed and sequenced with Illumina NovaSeq 6000  technology, using 150 bp paired-end settings and targeting an average of 20 million read pairs per library (<200nt) (GENEWIZ/Azenta)