GSM8665991: mm73_hsNUP98r_RNA_PF9363_3mpk-1mo_54d_REP2_231031_GGCTAC_S8; Homo sapiens; RNA-Seq
For RNA isolation from cell lines, 1x10^6 cells were lysed in 350 µl of RLT buffer and RNA was isolated using the RNeasy Mini Kit (Qiagen) according to manufacturer's instructions for cell line experiments. 1 ug RNA was used as the input quantity for library construction. Poly-A tail selection and library preparation were performed using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA).