18S rRNA gene sequencing of marine plankton:sample19
The V9 region of the 18S rRNA gene was amplified using primers 1389F and 1510R (Amaral-Zettler et al., 2009). PCR products were purified with the E.Z.N.A. Gel Extraction Kit (Omega, USA) and sequenced on an Illumina NovaSeq 6000 platform (Magigene, China).