GSM9268082: WI-38 fibroblasts, PDL54, CTCF ChIP; Homo sapiens; ChIP-Seq
Chromatin was prepared following ENCODE ChIP-seq guidelines, available here: https://www.encodeproject.org/documents/be2a0f12-af38-430c8f2d-57953baab5f5/@@download/attachment/Epigenomics_Alternative_Mag_Bead_ChIP_Protocol_v1.1_exp.pdf Experiments were performed to ENCODE standards. The antibody to H3K27ac used was a polyclonal from Active Motif: part number is 39133, lot number 31814008. The antibody to CTCF used was a polyclonal antibody from Diagenode: part number is C15410210, lot number is A2359-00234P. All commercial histone antibodies were validated by at least two independent experiments. Histone mark antibody standards are available at the ENCODE portal: https://www.encodeproject.org/documents/4bb40778-387a-47c4-ab24-cebe64ead5ae/@@download/attachment/ENCODE_Approved_Oct_2016_Histone_and_Chromatin_associated_Proteins_Antibody_Characterization_Guidelines.pdf    The quality of the ChIP-seq data was determined using the read depth, NRF, two PCR bottlenecking coefficients (PBC1 and PBC2), replicate concordance (i.e., IDR), and FRiP. https://www.encodeproject.org/documents/4bb40778-387a-47c4-ab24-cebe64ead5ae/@@download/attachment/ENCODE_Approved_Oct_2016_Histone_and_Chromatin_associated_Proteins_Antibody_Characterization_Guidelines.pdf