RNA-seq of E17.5 WT and Emb-/- kidney
RNA-seq of E17.5 WT and Emb-/- kidney
Five E17.5 Wild type and Emb-/- mouse kidneys were dissected. Total RNA was isolated by using the NucleoSpin RNA kit (Macherey-Nagel) according to the manufacturerâ€™s instructions. The libraries were prepared from 300 ng of RNA from each sample using TruSeq Stranded mRNA HT Kit and TruSeq Stranded mRNA Sample Preparation protocol 15031047 (Illumina)