GSM9284985: DMF Input; Homo sapiens; ChIP-Seq
Cells were fixed for 10 minutes with 1% formaldehyde and quenched  by adding Glycine (0.125M). Chromatin DNA was fragmented by sonication. RNA PolII was  immunoprecipitated with  antibody (clone CTD4H8; Millipore Sigma, Cat. No. 05-623-Z) overnight at 4°C. Next day, lysates were incubated in prewashed protein G dynabeads . After washing and elution, the protein–DNA complex was decross-linked and  DNA was purified by using QIAquick PCR purificaion kit. ChIP–Seq libraries were prepared using the KAPA Hyper Prep Kit (Roche, 7962347001) following the manufacturer's protocol, and sequenced on an Illumina NovaSeq X Plus platform.