16S amplicon sequencing of DNA ThickSlick Day 32 E2
DNA isolated from samples using the AllPrep Bacterial DNA/RNA/Protein Kit (Qiagen). DNA sequenced using Illumina MiSeq (San Diego, CA, USA) targeting the V4 region of the 16S rRNA gene (primers 515F & 806R) at the Cincinnati Children's Hospital Medical Center Genomics Sequencing Facility.