GSM9282571: 35bp+H3 DiNucleosome sense strand; synthetic construct; Bisulfite-Seq
The DNA was then immobilized on Agencourt AMPure XP Beads (Beckman Coulter), washed twice with 80% ethanol, eluted with water, and subjected to bisulfite conversion using the EZ DNA Methylation Kit (Zymo Research) following the manufacturer's instructions. Bisulfite-converted DNA was then amplified by PCR using the ZymoTaq DNA Polymerase (Zymo Research). PCR products were then sent out for next-generation sequencing (Genewiz) using the Amplicon-EZ service.