GSM8878682: C2C12 myoblasts-M3-DM4 (IP); Mus musculus; OTHER
Total RNA was isolated and purified using TRIzol reagent following the manufacturer's procedure. The RNA integrity was assessed with a Bioanalyzer 2100 with a RIN > 7.0 and confirmed by electrophoresis with a denaturing agarose gel. Poly(A) RNA was purified from 50 µg of total RNA using Dynabeads Oligo (dT) 25-61005. Then, the poly(A) RNA was fragmented into small pieces via the Magnesium RNA Fragmentation Module. Then, the cleaved RNA fragments were incubated with an m6A-specific antibody. Then, the IP RNA was reverse transcribed to create cDNA, which was subsequently fled to synthesize U-labeled second-strand DNAs. After ligation with the adapter to the A-tailed fragmented DNA, the ligated products were amplified with PCR.