ATAC-Seq of BAF60a beta-cell specific KO mus musculus injected with AAV-BAF60a: adult male pancreatic islet
Age-matched adult mice (8-12 weeks of age) were subjected to tamoxifen injection. Tamoxifen (Cayman Chemical, 10540-29-1, USA) was dissolved in corn oil (Sigma, C8267) at 20 mg/ml and administrated to mice through IP injection at 100 mg/kg body weight for five consecutive days. AAV-RIP-EGFP or AAV-RIP-BAFA60a were injected via pancreatic ductal infusion 4 months post-tamoxifen injection, and islets were isolated 4 weeks post-AAV injection. Then, 50,000 cells were extracted from isolated mouse islets by TrypLE (Thermo Fisher, 12604013). Cells were lysed with lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2 and 0.1% (v/v) Igepal CA-630) at 4C for 10 min. Tagmentation and amplification were done following the manufacturers instruction with TruePrep DNA library Prep Kit V2 (Vazymes, TD501). Paired-end sequencing was performed by Annoroad Gene Technology (Beijing, China).