GSM9406962: Wild-type HCT116, replicate2, H3K4me3; Homo sapiens; OTHER
For each reaction,  approximately 2.5×105 intact cells were crosslinked with 0.1% formaldehyde for 2 minutes and quenched with 125 mM glycine (pH 2.0). Cells were immobilized on Concanavalin A‑coated magnetic beads (BioMag® Plus Concanavalin A, Polysciences Inc. #86057), washed in Wash Buffer containing 0.1% Triton X‑100, and incubated with primary antibody or normal rabbit IgG overnight at 4°C. pAG‑MNase was added to bind chromatin‑associated antibody. MNase was then activated to cleave and release DNA fragments. DNA fragments were prepared for sequencing using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7103) based on the manufacturers recomandation. 15 cycles of PCR were used to amplify the libraries .