HiFi reads of Lindera aggregata
Prior to HiFi sequencing, DNA samples were first subjected to agarose gel electrophoresis to assess their quality (main band > 30 kb). High-quality DNA samples that pass the test were selected and randomly interrupted by a Covaris ultrasonic disruptor to bring the fragment size within the target range of 15-18 kb, and large fragments of DNA were purified using magnetic beads for enrichment. Subsequently, each PacBio single molecule real-time (SMRT) library was built using SMRTbell Express Template Prep Kit 2.0. The library was then sequenced in CCS mode on the Pacific Bioscience Revio platform, and high-fidelity (HiFi) reads were generated using Circular Consensus Sequencing (CCS) workflow v8.0.1. This process produced a total of 91.80 Gb data, with an average read length of approximately 17.22 kb, and an N50 read length of approximately 17.23 kb