RNA-seq of healthy seedling leaf and root tissue of seven legume species
RNA-seq of healthy seedling leaf and root tissue of seven legume species
At the three fully expanded leaf developmental stage, leaf and root tissues were harvested separately, immediately frozen in liquid nitrogen, and stored at -80°C until processing.  Plants were cultivated in 0.5 L pots containing growth medium (50% soil, 25% vermiculite, 25% peat) in controlled environment chambers at 22°C with a 12/12-hour photoperiod. Total RNA was extracted from approximately 100 mg of tissue using the GeneJET Plant RNA Purification Mini Kit (Thermo Scientific, Vilnius, Lithuania) according to manufacturer's protocols, with three biological replicates per tissue and species. mRNA library preparation was performed using poly A enrichment.