GSM9359380: Rat trigeminal ganglia following CCI-ION and SFM-Gel @NGC-Light groups 1; Rattus norvegicus; RNA-Seq
Total RNA was extracted using TRIzol reagent (Invitrogen) according to the manufacturer's protocol. RNA purity and concentration were assessed using a NanoDrop 2000 spectrophotometer (Thermo Scientific). RNA integrity was verified using an Agilent 2100 Bioanalyzer (Agilent Technologies). Sequencing libraries were constructed using the VAHTS Universal V10 RNA-seq Library Prep Kit (for Illumina) following the manufacturer's instructions. Briefly, mRNA was purified, fragmented, and reverse-transcribed into cDNA. After end repair, A-tailing, and adapter ligation, cDNA fragments were PCR-amplified. Final library quality and size distribution were validated.