RNA-Seq of Perilla: seed development
In this project, Perilla mRNA was enriched using Oligo(dT) magnetic beads, and RNA was fragmented into 300bp segments using ion fragmentation. After cDNA synthesis and PCR amplification, the samples were quality controlled using the Agilent Bioanalyzer, and finally, paired-end high-throughput sequencing was performed using the Illumina platform.