illumina iSeq100 paired sequencing  (KAS24163882) (KAE24105905)
To evaluate the efficiency of the optimized Cas-based 218 in comparison with base editors, purified genomic DNA samples were randomly fragmented. The generated library fragments predominantly ranged from 150 to 250 bp in size. Size-selected DNA fragments were amplified by PCR and subsequently purified, and each library was sequenced using the Illumina MiSeq platform. The sequencing process produced raw data for each sample in the form of paired-end reads, which were stored in FASTQ format.