RNA-Seq of  soybean rhizosphere soil microbiome: experimental group
Collect soybean rhizosphere soil samples from laboratory potted plants (experimental group) under salt alkali stress. Extract total genomic DNA from soil samples and amplify bacterial 16S rRNA genes using primers. Construct an Amplicon library according to the standard Illumina library preparation protocol and perform paired end sequencing on the Illumina platform to analyze the composition of rhizosphere microbial communities.