GSM8773179: Multiome_wt_96h_scATAC; Mus musculus; ATAC-seq
Gastruloid at 96h were collected, pooled and dissociated. Nuclei isolation for the single-cell Multiome ATAC and gene expression procedure was per-formed following the manufacture's instruction of the Chromium Nuclei Isolation Kit (10X Genomics). Dissociated cells were resuspended in cold lysis buffer and incubated for 10min on ice, then transferred to the nuclei isolation column and centrifuged at 16'000 rcf for 20sec at 4°C to re-move cellular debris. The Flowthrough was centrifuged to retrieve the nuclei pellet, then washed with cold Debris Removal Buffer and two rounds of centrifugation, and wash with cold Wash Buffer. Purified nuclei were resuspended in cold Resuspension Buffer, counted and their nuclear membrane assessed. Samples were immediately processed according to 10X Genomics recommendations of the v1.0 Multiome ATAC + Gene Expression kit. The 10X ATAC and GEX libraries were sequenced