GSM9282691: Reamp-EGFP-BC; Mus musculus; RNA-Seq
E11.5 mouse embryos with DNA barcode-linked EGFP were minced and enzymatically into single cell. Four mouse embryos were multiplexed with CellPlex technology (10x Genomics). Gel-bead-in emulsions (GEMs) were generated by Chromium controller (10x Genomics). cDNA library was condtructed using Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (10x Genomics) following the manufacturer's protocol. Transgene loci were amplified from scRNA-seq cDNA library using transgene-specific primers with illumina index sequences.