GSM9192510: WT_G1; Curvularia clavata; RNA-Seq
Total RNA was isolated using TRIzol reagent (Invitrogen, Carlsbad, CA, USA), treated with DNase I and purified using a Qiagen RNeasy Mini Kit (Qiagen, Hilden, Germany). RNA integrity was confirmed by agarose gel electrophoresis and an Agilent Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). Qualified RNA with A260/A280 > 2.0 and RNA integrity number (RIN) > 8.0 was used for RNA-Seq. The complementary DNA libraries were constructed and sequenced using the Illumina HiSeq 2000 platform by Majorbio (Shanghai Majorbio Bio-Pharm Technology Co., Ltd., China).