GridION sequencing of SAMD00826864
The DNA solution obtained by CTAB purification was prepared at a concentration of 150 ng/ml and fragmented by mixing it 10 times using an 18G syringe. Short fragments of DNA less than 40 kb were then removed using the Short Read Eliminator XL (Circulomics). The quality of the resulting DNA was checked with the Pippin Pulse (Sage Science). A library was prepared using the Ligation Sequencing Kit (SQK-LSK109, Oxford Nanopore) and the Flow Cell Priming Kit (EXP-FLP001 and EXP-FLP002, Oxford Nanopore).