Identification of papillomaviruses and polyomaviruses from Antarctic penguin feces and cloacal swabs
1 ml of the UTM viral transport media was filtered through a 0.22 m pore-sized syringe filter. From the resulting filtrate, 200 l was taken to extract viral DNA using the High Pure viral Nucleic Acid Kit (Roche Diagnostics, USA) following the manufacturers instructions. Rolling circle amplification (RCA) was performed to enrich the circular DNA molecules from the viral DNA purification using the Templiphi 100 Amplification Kit with DNA Polymerase Phi29. An equal ratio of purified viral DNA together with its respective RCA-enriched material was used to generate Illumina sequencing libraries using the Illumina DNA library preparation (M) tagmentation kit (Illumina, USA) and sequenced on an Illumina NovaSeq X Plus sequencer (Illumina, USA) at Psomagen Inc. (USA).