16r RNA seq of plasma: adult female
The sequencing libraries were quantified using Qubit, equal amounts of each were pooled together and the concentration of the pool was verified with an Illumina KAPA Library Quantification qPCR assay (Roche, Bcfasal, Switzerland). The pooled libraries were sequenced using an Illumina MiSeq system  to generate paired-end, 300 base pair sequence reads from the libraries in a multiplexed fashion.