GSM9511055: T cells from Donor 3 treated with a Cas9 targeting AAVS1; Homo sapiens; Bisulfite-Seq
Genomic DNA extraction by Maxwell RSC Cultured cells DNA Kit (Promega) and bisufite conversion by EpiTect Bisulfite Kit, following provider indications. Targeted genomic regions were amplified by PCR using locus-specific primers. A second round of PCR was performed to add full-length Illumina adapters and sample-specific barcodes.