ATAC-Seq of mus musculus: ILC2_ns_rep3
cells were washed twice with PBS and lysed in 50 ml lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630) following a centrifugation at 600 g for 10 min at 4 C. The pellets were resuspended in 50 ml transposition mix (25 ml TD buffer, 4 ml Tn5 transposase, 21 ml Nuclease free water; from Nextera DNA Library Preparation Kit, Illumina) and incubated in water bath at 37C for 30min. Tagmented DNA was purified by using MinElute PCR Purification Kit (Qiagen) and amplified with 10 or 11 cycles of PCR based on the amplification curve. The libraries were purified by dual selection using AMPure XP beads and then sequenced by Novogene using Illumina NovaSeq 6000 for 150bp paired-end sequencing.