GSM8493588: NUGC-3 Xenograft, vehicle, 2QDx1, 48h, bio rep 3; Homo sapiens; ssRNA-seq
Total RNA was extracted from the liquid nitrogen flash-frozen tumor samples by TissueLyser LT (Qiagen), and purified by using QIAcube (Qiagen) with DNase I digestion, following the manufacturer's protocols. After rRNA depletion by using Fastselect HMR kit (Qiagen), strand-specific RNA sequencing libraries were prepared by using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB), following manufacturers' instructions.