GSM9039049: R8-Lt-D; Oryctolagus cuniculus; RNA-Seq
To obtain RNA from the drilling site and implant-bone contact area, a curette was used to scrape the inside of the drilled holes in the rabbit tibiae after the implant had been removed from the bone section. The tissue scraped from the drilled site or implant-bone contact area was used for analysis of total RNA, which was isolated using TRIzol reagent. The extracted RNA was then treated with DNase I and purified using the miRNeasy Mini Kit (Qiagen, Hilden, Germany). Sequencing libraries were then prepared using the TruSeq stranded Total RNA library preparation kit, and paired-end sequencing (2 × 75 bp) was performed on the Illumina HiSeq 2500 platform (Illumina In., San Diego, CA, USA).