GSM8641966: epithelium, IgG, rep 2; Homo sapiens; OTHER
Initially, sample preprocessing involved the conversion of samples into cell suspensions, followed by cell collection and enumeration. Subsequent steps included cell washing, primary antibody binding, and subsequent addition of secondary antibodies. Following secondary antibody binding, the product underwent washing and interaction with ChiTagTM transposase. Sequencing of the library was conducted on the Illumina platform. For qualified libraries, NaOH was utilized to denature the library into single strands. The denatured library was diluted to a specific concentration based on the expected data volume and then introduced to the FlowCell. Hybridization of the denatured and diluted library with adapters on the FlowCell was followed by bridge PCR amplification on the cBot cluster generation platform, culminating in sequencing on the designated platform.