Long-read sequencing of Pythium oligandrum GAQ1
For Nanopore long-read sequencing, the gDNA was randomly sheared using a Megaruptor (Diagenode), and large DNA fragments were size-selected and purified using the BluePippin instrument (Sage Science) with magnetic bead cleanup. Damage to the fragmented DNA was repaired after purification, with both ends of the DNA fragments end-repaired and dA-tailed, and ligated with sequencing adaptors using the Ligation Sequencing Kit (Oxford Nanopore Technologies, Cat# SQK-LSK109). The DNA library was quantified using Qubit fluorometry and sequenced using a Nanopore PromethION sequencer for real-time single-molecule sequencing. MD5 Checksum: pass.fq.gz (93c43291af9314e25c383cad7a286067).