GSM8975985: DOX4X bio rep 4 female; Mus musculus; RNA-Seq
Total bone marrow were flushed out by centrifugation, followed by enzymatic digestion at 37°C for 25 min. Bone marrow mesenchymal stem and progenitor cells were sorted based on tdTomato expression into Buffer RLT Plus with 1% beta-mercaptoethanol. Total RNA was extracted with RNeasy Plus Micro Kit (QIAGEN, Cat. # 74034). The cDNA libraries were prepared using SMART-Seq v4 Plus Kit (Takara, Cat. # R400752) following manufacturer's protocol.