NovaSeq X 100 paired Sequencing (KAS24120759) (KAE24095398)
Two types of libraries composed of random sequences were generated, followed by in vitro transcription to produce mRNA. After mRNA transfection, the samples were subjected to sucrose gradient ultracentrifugation to obtain polysome fractions. The fractions were then separated into subpolysome and polysome fractions, from which libraries were subsequently prepared.