GSM9626240: Curcumin_Replicate_1; Caenorhabditis elegans; RNA-Seq
Following treatment, worms were harvested on Day 3 of adulthood, washed twice in cold M9 buffer, and resuspended in 10–20 µL residual buffer. RNA was extracted using QIAzol lysis reagent and a freeze-thaw method following RNeasy Universal Mini Kit (QIAGEN) guidelines, with final elution in RNAse-free water. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, first strand cDNA was synthesized using random hexamer primers, followed by second strand cDNA synthesis. The library was finalized after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.