GSM9449352: mouse bone, microgravity; Mus musculus; RNA-Seq
The mice in the TCU were launched using SpaceX-22 from the NASA Kennedy Space Center (KSC) on June 3rd, 2021 (Day 0) and then transferred to Kibo, the Japanese experimental module attached to the International Space Station (ISS). On Day 9 or Day 10, the mice were starved for 5-16 hours, anesthetized by intraperitoneal injection of Ketamine (150mg/kg) and Xylazine (45 mg/kg), and then sacrificed by whole blood sampling with cardiac puncture and cervical dislocation. The carcasses were stored in the -95C freezer. The frozen blood samples and carcasses arrived at KSC on July 20th, 2021 via spaceX-22. The difference in the food weight between before and after the space flight was measured to calculate the average daily food consumption. As a control group for this microgravity group, mice were housed on ground under the same conditions as in the space (the normal gravity group). Six (6) mice with the similar body weight as those sent to the ISS were selected and transferred individually to the TCU. These mice were fed the same amount of food each day as the average daily food intake of the microgravity group. The carcasses were prepared 9 or 10 days later and stored frozen in a -80C freezer. The bone RNA samples prepared individually from 6 mice each in the microgravity group and the normal gravity group were mixed in equal amounts per group, and subjected to RNA sequencing analysis. Sequencing libraries were constructed using TruSeq Stranded mRNA Sample Prep Kit (Illumina).