GSM9587621: GWAT_female_TWD_replicate.4; Mus musculus; RNA-Seq
We extracted RNA from F1 gWAT and liver using a VWR® Micro Homogenizer (catalog number #10032-328) and Qiagen RNeasy Plus kits (catalog number #74034), following the manufacturer's protocols. We submitted 100 RNA samples (2 sexes x 2 tissues x 5 experimental groups x 5 replicates per group) to the University of California, Irvine (UCI) Genomics High Throughput Facility for sequencing using Illumina TruSeq Stranded mRNA kits and an Illumina NovaSeq 6000 sequencer. Libraries obtained from were sequenced up to three times to yield approximately 30 million 150-bp paired reads per sample.