GSM9510509: H3K4me3_CCT251921_72h_repl_3; Homo sapiens; OTHER
The Cut&Tag assay was performed on 300,000 Tex cells for each antibody, following the manufacturer's recommendations (CUT&Tag-IT™ Assay Kit, Active Motif, Catalogue No. 53165 or 53171). For the experiment, we used the following antibodies: H3K27ac (Active Motif, cat. no. 91193); H3K4Me1 (Active Motif, cat. no. 39635); H3K4Me3 (Active Motif, cat. no. 39060); RNAP2 CTD (Abcam, cat. no. Ab5095); MLL-4 (Millipore, cat. no. ABE1867); CDK8-500 (Bethyl, cat. no. A302-500); BRD3 (Invitrogen, cat. no. PA530263); NFRKB (Bethyl, cat. no. A301-459A). According to CUT&Tag-IT™ Assay Kit (Active Motif, Catalogue No. 53165 or 53171)