NGS sequencing of CYXGZ: leaf
a 350 bp paired-end library was constructed following manufacturer protocols (Illumina). DNA fragmentation was conducted by sonication, followed by end-polishing, A-tailing, and full-length adapter ligation. PCR-amplified products were purified using the AMPure XP bead system. Library size distribution was assessed via an Agilent 2100 Bioanalyzer, and quantification was performed by real-time PCR prior to sequencing on the Illumina NovaSeq X Plus platform.