GSM8880358: Sample_T1_032521_S8 (5 cell lines); Homo sapiens; RNA-Seq
Cells from all five iPSC lines were pooled into one library per time point by cell hashing94 with TotalSeq-A Hashtag antibodies (BioLegend). The libraries were generated with Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (10 × Genomics). We prepared three libraries (three technical replicates) from pooled cells for each time point. Libraries were sequenced on an Illumina NovaSeq 6000 (SP, 2×150 bp).