GSM9192987: siZFPM2AS1,RRBS; Homo sapiens; Bisulfite-Seq
Genomic DNA was extracted using the DNA Extraction Kit (DP304, Tiangen). DNA was digested with the restriction enzyme MspI to enrich for CpG-rich regions, followed by end-repair, A-tailing, and ligation with methylated adapters to preserve methylation information during subsequent steps. Size-selected fragments were then subjected to bisulfite conversion using the EZ DNA Methylation-Gold Kit (Zymo Research) to convert unmethylated cytosines to uracil. Adapter-ligated fragments were amplified by PCR to generate the final RRBS libraries, which were quality-checked prior to sequencing.