16S rRNA of microbial community in seawater: ambient water
16S rRNA and rDNA sequencing was conducted using primers 515F and 926R (Walters et al., 2015) as further described by Givati et al. (2024). In short, amplicons were generated using a two-stage PCR protocol, and libraries were sequenced on an Illumina MiSeq platform (V3 chemistry, 2  300 bp reads) with a 15% phiX spike-in. Library preparation and sequencing were performed at the Genomics and Microbiome Core Facility, Rush University, IL, USA.