GSM8525101: HCT-G334R-Nutlin-p53-ChIP-2; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes then quenched with glycine. After cell lysis, chromatin was sonicated using a a focused-ultrasonicator (Covaris, ME220). Libraries for ChIP-seq were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) as per manufacturer's instructions starting with 1 ng of ChIP DNA and 12 cycles of final PCR amplification. Library size was assessed using the 4200 Tapestation and the High-Sensitivity DNA assay (Agilent, Santa Clara, CA).  Concentration was determined using the Qubit Fluorometer 2.0. (Thermo Fisher Scientific, Waltham, MA).