GSM9191987: B12+Butyrate; Mus musculus; RNA-Seq
Three viably frozen bone marrow samples from separate mice were pooled per treatment group were flow cytometry sorted for live (DAPI-) CD45.2+ cells. Samples were also sorted by cKit marker expression. After sorting, cells were pooled 50:50 cKit+:cKit- per treatment group. Library preparation was performed using 10X Genomics Chromium Next GEM Single Cell 3' Reagents Kit (v3.1) according to manufacturer's instructions.