WGS of Mycobacterium tuberculosis: type strain
Genomic DNA of H37Rv and H37Rv-S strains were extracted using CTAB (cetyltrimethylammonium bromide) according to a standard protocol, then were used to build the DNA library by DNA Library Prep Kit. The quality of DNA library was assessed by Qubit 2.0, and the inserted fragments were measured using Agilent 2100. After detection qualified, the DNA library was sequenced on the Illumina NovaSeq 6000 platform (Illumina, Inc, San Diego, CA) according to the manufacturers specifications.