Aspergillus treated female fat body
Healthy female adult locusts were inoculated with 1  107 conidia mL1 suspensions. Female adults were collected from the boxes for RNA extraction. Fat bodies were collected by dissecting the adults on a waxed Petri dish. All collected fat bodies were transferred to Trizol reagent for RNA extraction. RNA was extracted using the Trizol Reagent per the manuals instructions. they RNA were sent to company Illumina sequencing. A total of four replicates of treated individuals were performed for the female fat body samples at each time interval. Fat bodies were collected from 610 female adults. A total of four boxes were considered one replicate in the fat body extraction experiment.