GSM9589365: placenta DNA, MARBLES, male 8; Homo sapiens; Bisulfite-Seq
Placenta samples underwent DNA isolation via the Gentra Puregene kit (Qiagen, Hilden, Germany) by following recommended procedures and quantified with the Qubit DNA Assay Kit (ThermoFisher Scientific, Q32851). DNA was sheared by sonication, bisulfite-converted with the EZ DNA Methylation Gold kit (Zymo, Irvine, CA, USA) and made into sequencing libraries via the xGen Methyl Seq Library Prep kit (IDT, Coralville, IA, USA). Libraries were made with indexed PCR primers and underwent 7 cycles of amplification before being pooled and sequenced for 150bp paired end reads on 4 lanes of the NovaSeq 6000 S4 (San Diego, CA, USA)  by the DNA Technologies Core at the University of California, Davis (Davis, CA, USA).