16S_DNA_Gut_HiLo
Platform: MiSeq; Paired  end: Yes; Read length (bp) : 300; Primer pair: V3-V4: 341F/805R; DNA polymerase :  Kapa HiFi Hot Start Ready Mix;PCR cycle No. : 25; Adapters:Nextera XT ;Sequencing depth  (avg reads per sample): 308367; Library prep and sequencing protocol: Illumina 16S Metagenomic Sequencing Library guide (v. 15044223-B)