Targeted sequencing of blood from breast cancer patients
DNA was extracted using the Autopure LS automated DNA extractor (QIAGEN, Hilden, Germany) and quantified using Qubit (Invitrogen, Waltham, MA); DNA libraries were constructed using a custom designed SureSelectXTHS2 kit (Agilent, Santa Clara, CA), which optimally captured whole exons of 84 hematopoietic disorder-related genes, including single nucleotide mutations and small indels; libraries were sequenced per manufacture's recommendations with a goal of achieving an average depth of coverage >5,000x.