ONT sequencing of a single Chrysomya bezziana male
Genomic DNA was extracted from a single frozen male fly using the CTAB method (Hunt 1997). The DNA was initially quantified using Nanodrop 2000 (Thermo Scientific) and Qubit dsDNA BR Assay Kits. DNA integrity was assessed with 4150 TapeStation (Agilent). DNA was repaired generating 5' phosphorylated, 3' dA-tailed ends. The repaired DNA was ligated with ONT-compatible adapters. Sequencing was performed using GridION (Oxford Nanopore Technologies).