PacBio sequencing of SKNO-1 cell line G
Frozen SKNO-1 cell pellets (5 x 10^6 cells each) from the DSMZ were submitted to the Translational Genomics Research Institute (TGen) for high-molecular-weight DNA extraction using the NEB Monarch HMW Extraction Kit for Cells & Blood. DNA quality was assessed using the Agilent Femto Pulse, followed by shearing using the Megaruptor 3 (Diagenode). Library preparation was performed using the SMRT-Bell Prep kit V3, and sequencing was carried out on the PacBio Revio system using SMRT-Cell technology, targeting an estimated 40X coverage for the human genome from each cell line.