Illumina sequencing of Shewanella oneidensis: conditional chaperone mutant
A conditional Shewanella oneidensis chaperone null mutant was used to test the dependency of outer membrane proteins on canonical chaperones. The cells were grown in anoxic LB medium containing 100 mM fumarate and 1 mM IPTG to induce chaperone expression. The culture was then used to isolate genomic DNA for Illumina sequencing, as well as for an experiment carried out with and without chaperone expression.