GSM8493520: Endothelial cells, miR-187, rep1; Homo sapiens; ATAC-seq
Extract DNA using the ATAC seq library preparation kit (APExBIO) extraction kit ATAC-seq libraries of cells were prepared as the manufacturer's protocol (Cat. No. K1157, APExBIO). 50,000 cells of samples were lysed in 50 μL ice ATAC Lysis Buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2) containing 0.5 μL 10% NP40, 0.5 μL 10% Tween 20, and 0.5 μL 2% Digitonin for 3 min. Lysates were spun at 1000 rpm for 10 min to obtain the pellet nuclei. The nuclei were then incubated with the transposase enzyme and tagmentation buffer at 37 °C for 30 min. The fragmented DNA was then purified using MinElute Reaction Cleanup Kit (Cat No. 28204, QIAGEN) in a final volume of 10 μL. ATAC-Seq Libraries were prepared following the Buenrostro protocol (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4374986/). The DNA libraries were amplified for 10 cycles and sequenced using Illumina NovaSeq 6000 with paired-end 2×150 as the sequencing mode.