GSM8744540: PANC1-siNeg, rep1; Homo sapiens; RNA-Seq
Total RNA from each sample was extracted using the RNeasy Mini Kit (Qiagen GmbH, Hilden, Germany). The yield of RNA was first quantified by measuring total RNA concentration using a Qubit™ 2.0 fluorometer (Thermo Fisher Scientific, USA) with Qubit™ RNA HS Assay Kit (Thermo Fisher Scientific). The quality of the extracted RNA was estimated using the Agilent High Sensitivity RNA Screen Tape using the TapeStation 2200 (Agilent Technologies, Santa Clara, CA, USA). PolyA RNA isolation was performed with the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Ipswich, MA, USA). The NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Bi-olabs) and the NEBNext Multiplex Oligos for Illumina (Index Primers Set 3, 4) were used to prepare the libraries.