Methanospirllum lacunae Ki8-1
DNA was extracted using the Zymo D6060 Quick-DNA HMW Magbead Kit per the manufacturers instructions and fragmented using Covaris gTubes following instructions from the manufacturer. Average size of the sheared gDNA was checked at the TapeStation 4200 (Agilent).  Multiplexed microbial libraries were prepared using the PacBio SMRTbell prep kit 3.0 together with the SMRTbell barcoded adapters 3.0 according to the PacBio protocol.