HiSeq X Ten paired Sequencing of single_cell (KAS24117303) (KAE24092863)
Single-cell RNA-seq libraries were prepared following the 10x Chromium Single Cell 5&#39; v2 protocol (10x Genomics, CG000331) using the Chromium Controller and Next GEM Single Cell 5&#39; Reagent v2 Kit. A suspension of ~10,000 cells was mixed with reverse transcription reagents and loaded onto a Single Cell K Chip along with Gel Beads and Oil to form Gel Bead-in-Emulsions (GEMs). Inside each GEM, cellular RNA was barcoded and reverse-transcribed into full-length cDNA. The cDNA was then amplified and processed into libraries through fragmentation, end-repair, A-tailing, adapter ligation, and index PCR.