18S V4 amplicon sequence collected from Sabina Shoal (SAB05) on April 28, 2019
Seawater samples were collected 1-2 m below the surface using a Niskin water sampler. The >=3 um and non-fractionated portions were used to focus on the larger taxa (nano-microeukaryotes). Total genomic DNA was extracted using the DNeasy PowerSoil Kit following the manufacturers protocol. The samples were sent to the Integrated Microbiome Resource in Dalhousie University, Canada for Illumina multiplex high throughput sequencing (HTS). HTS was done using the E572F and E1009R primers to specifically target the V4 region of the 18S rRNA gene.