GSM8743414: tRNA_m3C_IP2; Homo sapiens; OTHER
Total RNA was isolated from cell lines by Trizol(Invitrogen).tRNA was purified by 7M 10% denaturing polyacrylamide TBE-urea gel. polyA+ RNA was purified using Dynabeads Oligo(dT)25 (Invitrogen). polyA+ RNA RNA fragments or tRNAs were converted to cDNA with high-processive reverse transcriptases SuperScript IV (Invitrogen) and sequenced (Input), or precipitated by m3C antibody before cDNA synthesis (IP), and the use of demethylase treatment improves the confidence of detection (Dm).