GSM8743017: Retinoic acid-induced N2a cells , RSL3-3; Mus musculus; RNA-Seq
Total RNA was extracted using the TRIzol reagent (Invitrogen/Life Technologies) following the manufacturer's protocol. RNA concentration was quantitated using the NanoDrop ND-100 Spectrophotometer (NanoDrop Technologies, Wilmington, DE). rRNA were removed using NEBNext® Poly(A) mRNA Magnetic Isolation Module. RNA libraries were constructed using KAPA Stranded RNA-Seq Library Prep Kit and following standard protocol. Individual libraries were constructed using 1µg of total RNA isolated from each sample. 1) 3'-adaptor ligation;2) 5'-adaptor ligation;3) cDNA synthesis;4) PCR amplification.