GSM8880119: Endothelial Cell, Late G1, SOX17 antibody; Homo sapiens; OTHER
HUVEC-FUCCI were generated by lentiviral infection of the FastFUCCI plasmid (pBOB-EF1-FastFUCCI-Puro was a gift from Kevin Brindle & Duncan Jodrell (Addgene plasmid # 86849 ; http://n2t.net/addgene:86849 ; RRID:Addgene_86849)). HUVEC-FUCCI were cultured in EGM-2 media on 10 cm tissue culture plates pre-coated with 0.1% Gelatin. CUT&RUN for library preparation of SOX17-bound chromatin was performed on HUVEC-FUCCI that were sorted by FACS into early G1 or late G1 using the CUT&RUN Assay Kit with anti-SOX17 antibody. Prepped libraries were submitted to Novogene for library pooling and next-generation sequencing.