GSM8879377: SC-A Replicate 3; Homo sapiens; RNA-Seq
Differentiated SC-α clusters were washed twice with ice cold PBS and then dispersed using TrypLE (10 minutes at 37 °C). TrypLE reaction was quenched with 2% BSA containing media and cells were centrifuged (200 xg, 5 min, 4 °C). Cells were washed with ice cold PBS, re-centrifuged, and resuspended in fresh ice cold PBS. Cells were counted and diluted to a concentration of 0.5 million cells/mL in ice cold PBS. The 10x Genomics Single Cell 3' v3 library was constructed for according to manufacturer guidelines.