GSM8587252: Undifferentiated_PGC_rep3; Gallus gallus; RNA-Seq
1 μg of total RNA was purified using FastGene™ RNA Premium kit (NIPPON Genetics Co. Ltd., Tokyo, Japan) from each PGC line. mRNA was enriched from the total RNA using NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Ipswich, MA, USA), and cDNA libraries were constructed using SMARTer® Stranded RNA-Seq Kit (TaKaRa Bio, Kusatsu, Japan) and MGIEasy Universal Library Conversion Kit (MGI, Shenzhen, China).