eTnpBc_OsHMBPP_leafrep4
Leaf extract was used directly for polymerase chain reaction (PCR) with Phire Plant Direct PCR MasterMix (Thermo Scientific) according to manufacturer instructions. PCR products were cleaned with Ampure XP beads (Beckman Coulter), analyzed by a 4150 TapeStation (Agilent), and submitted for Illumina sequencing.