GSM8901774: WT gene expression; Mus musculus; RNA-Seq
total splenocytes were purified as for flow cytometry, followed by negative selection with biotinylated Ter-119, anti-biotin microbeads, and magnetic separation (Miltenyi). Following single-cell separation on the 10x Chromium system and cDNA generation, libraries were generated using the 5'mRNA and V(D)J kits, then sequenced using PE100 sequencing on DNBSEQ.