16S soil microbiome
PCR amplification of V3 V4 16S was performed by using specific primers connecting with barcodes. The PCR products of proper size were selected through agarose gel electrophoresis. The same amount of PCR products from each sample was pooled, end-repaired, A-tailed, and further ligated with illumina adapters. Libraries were sequenced on a paired-end Illumina platform.