GSM8774599: 60% HFD plus 0.25% HDCA; Mus musculus; RNA-Seq
Single-cell hepatocytes were prepared using the collagenase perfusion method. The scRNA-seq libraries were prepared using 10× Genomics Chromium Next GEM single-cell 3ʹ reagent kits. Cell suspensions were loaded onto the 10× Genomics Chromium Controller to generate gel beads in emulsion (GEM) for polyA cDNA synthesis with cell barcodes and unique molecular identifiers (UMI). GEMs were broken, and single-stranded cDNA was cleaned with DynaBeads, amplified, and assessed for quality using an Agilent TapeStation.