scRNA-seq of murine SI LP DCs
Dendritic cell (DC)-enriched (negative selection) small intestinal lamina propria (SI LP) cells from two CCR7gfp/+ mice were pooled; 36000 GFP- and 12000 MHCIIhi GFP+ SI LP were sorted using a FACSAria Fusion (BD). Cells were further processed using the Chromium Next GEM Chip K and the Next GEM Single Cell 5 Kit, and library was prepared using the Library Construction Kit according to the Chromium Next GEM Single Cell 5-v2 User guide (10x Genomics; RevD steps 1, 2 and 5). Sequencing was performed on a NextSeq 500 sequencing system (Illumina).