GSM8742318: dTAGMTL3-0hr-2; Mus musculus; RNA-Seq
RNA extraction for RNA-seq was performed by combination of Trizol reagent (ThermoFisher Scientific) and RNeasy mini kit (QIAGEN) RNAs were sent to University of Michigan DNA Sequencing Core for RNA quality analysis, PolyA+ mRNA selection and library construction, and sequencing using Illumina NovaSeq S4 Libraries platform. Duplicate samples for each group were subjected to two round of independent library preparation and sequencing to avoid sample and batch effect of mRNA-seq.