GSM8525908: ARfl_siNTC_VC16 rep3; Homo sapiens; ChIP-Seq
Cells were crosslinked with double fixation. Cells were first crosslinked in 2 mM fresh disuccinimidyl glutarate for 35 minutes, followed by 1% FA for an additional 10 minutes. The samples were sonicated using the Covaris E220 in sarkosyl lysis buffer. ChIP-seq libraries were generated using the SimpleChIP ChIP-seq Multiplex Oligos for Illumina with Dual Index Primers (Cell Signaling Technology, cat# 47538) according to the manufacturer's instructions. Libraries were pooled and sequenced using a NovaSeq 6000 Sequencing System.