Twist-ONT: Combining Nanopore Sequencing with the Twist Comprehensive Viral Research Panel
DNA and RNA was extracted using EMAG instruments and NUCLISENS easyMAG reagents. RNA was converted to cDNA using Lunascript and Sequenase. DNA was amplified and viral sequences was enriched using the Twist-ONT protocol which uses PCR and the Twist Comprehensive Viral Research Panel. The Oxford Nanopore Technologies Ligation Sequencing Kit was used for library preparation.