Chemo-mechanical changes of low-pH concrete under deep geological repository-like conditions
To identify microbial community, amplicon sequencing of 16S rRNA was employed with inhouse library preparation protocol using 515F primer  TGCCAGCMGCNGCGG and 802R primer  TACNVGGGTATCTAATCC targeting bacterial V3-V4 regions. Amplicons were purified using the Agencourt Ampure XP system (Beckman Coulter, Brea, USA). Barcoded sequencing adapters were attached to the PCR products using the Ion Xpress Plus gDNA Fragment Library Kit along with Ion Xpress barcode adapters (Thermo Fisher Scientific, Waltham, MA, USA). Sequencing was carried out using an Ion PGM Hi-Q Sequencing Kit on an Ion 314 Chip (Thermo Fisher Scientific, Waltham, MA, USA).