GSM8664531: L. albus 40 min 10 mM sucrose  replicate 3; Lupinus albus; RNA-Seq
RNA from frozen root samples was isolated using the RNeasy Plant Mini kit (Qiagen, Valencia, CA, USA). TapeStation (Agilent, Santa Clara, CA, USA) was used to assess both quantity and quality of each sample; only RNA samples with RIN (RNA integrity Numbers) above 8 were used for RNA-seq. The Stranded mRNA Kit (Illumina, Foster City, CA, USA) was used to convert the extracted RNA to cDNA, following the manufacturer's instructions. Unique dual bar-coding for each cDNA library was done using the IDT for Illumina RNA UD Indexes Set A (Illumina). Quantity and quality of cDNA libraries were assessed via TapeStation (Agilent, Santa Clara, CA, USA) and remaining adaptors were cleaned up where necessary using AMPure XP magnetic beads (New England Biolabs, Ipswich, MA, USA) at a ratio of 1 volume DNA to 0.8 volumes of beads. Exact quantification, pooling, and sequencing of the 12 barcoded cDNA libraries was performed at QB3 (Institute for Quantitative Biosciences at UC Berkeley). Sequencing of the pooled cDNA libraries was performed on one flowcell lane on the Illumina No-vaSeq6000 SP 150PE (paired-end) next-generation sequencing platform.