GSM8902711: KO01; Mus musculus; RNA-Seq
Mesenteric adipose tissue was collected and enzymatically digested at 37°C for 30 minutes in Hank's Balanced Salt Solution (HBSS) containing Liberase TL and DNase I. Tissues were pipetted up and down to promote dissociation, then filtered and subjected to red blood cell lysis. Single cell suspension was stained using CD45-PE and CD31-BV421. Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (Dual Index) from 10x Genomics