GSM8742016: Kat6a WT Kat6b Tg, rep 3; Mus musculus; RNA-Seq
Total RNA from whole E9.5 embryos was extracted using an RNA extraction kit (Qiagen RNeasy mini kit; Qiagen, 74104), according to the manufacturer's instructions and including the optional DNaseI digestion step. RNA quality and quantity were assessed on an automated analyser (Tapestation 4200; Agilent, G2991BA) 500 ng RNA were used to generate libraries using a library construction kit (TruSeq RNA prep kit v2; Illumina, RS-122-2002), according to the manufacturer's instructions.