RNA-Seq of mus musculus: 4T1
Cells were preserved in Trizol at -80C for RNA extraction. Total RNA was extracted using Trizol reagent kit (15596018, Invitrogen) according to the manufacturers protocol. The total RNA samples were transported on dry ice to Gene Denovo Biotechnology Company (Guangzhou, China) for library construction and bulk-sequencing (Illumina Novaseq6000).