whole genome sequencing of the strain
A single colony was used to inoculate 5 ml Hv-Cab liquid culture supplemented with tryptophan and uracil at a final concentration of 50 ug/ml. Cells were grown at 45 C in an orbital shaker at 250 rpm with an orbital diameter of 2.54 cm until it reaches OD600 of 0.6. Genomic DNA was extracted using the GeneJET PCR Purification Kit from Thermo Scientific and quantified using a Qubit 3.0 Fluorometer from Invitrogen. The genomic DNA was then further analyzed by Illumina whole genome sequencing performed by SeqCenter (Pittsburgh, PA, USA).