GSM8807133: Hippocampus_NLGF_W3_scATAC_Female.pool; Mus musculus; ATAC-seq
Hippocampal nuclei were isolated by pooling tissue from five male and five female replicates per age/genotype group, yielding 12 pools. Nuclei were extracted via homogenization, filtration, and centrifugation. Extracted nuclei were tagmented with Tn5 transposase and washed. Multiplexed nuclei were processed using the 10x Genomics Single Cell ATAC v2 Kit, incorporating ScaleBio indexing. Libraries were quantified and sequenced on the Illumina NextSeq2000 with paired-end reads.