Oxford Nanopore Pore-C of Burkitt Lymphoma cell line DG-75
10 million cells were crosslinked with 1% formaldehyde (10 min, room temperature) and quenched with 1% glycine. Half the cells underwent permeabilization, ligation, and denaturation, yielding ~15 g DNA. Libraries were prepared from 1 g DNA using the ONT LSK114 kit and washed with the Long Fragment Buffer to exclude smaller DNA fragments. The library was loaded onto Oxford Nanopore promethION flow cells for sequencing with washing and reloading 1-2 times.