GSM8646092: day12 replicate2; Mus musculus; RNA-Seq
Samples (day0 replicate1, day3 replicate1, day5 replicate1, day7 replicate1, day5 replicate2, day7 replicate2 and day12 replicate2), were collected, dissociated with 0.25% trypsin-EDTA, deactivated with mESC media without inhibitors (2i/Lif). Cells were collected, dissociated, centrifuged, resuspended in 1X PBS supplemented with 1% BSA, and filtered through a strainer. Samples were multiplexed using CellPlex, according to the manufacturer's recommendations. Samples (Day2budoids and Day5budoids) were collected, washed with 1X PBS, dissociated with 0.25% trypsin-EDTA and deactivated with mESCs media without inhibitors (2i/Lif). Cells were collected, centrifuged, resuspended in 1X PBS supplemented with 0.04 % BSA and filtered. Please refer to the main text for more details. Library was performed according to the manufacturer's instructions (3'GE v3.1).