GSM8880161: WT RNA-seq, untreated, biol rep 1; Haloferax volcanii; RNA-Seq
RNA extraction was performed using a Quick-RNA Miniprep kit (Zymo). rRNA was depleted using 2.5 µg of DNase-treated RNA and the Ribo-Zero rRNA removal kit for bacteria (Illumina, discontinued). The SMARTer Stranded RNA-Seq kit (Takara Bio) was used to prepare the libraries with 100 ng RNA, dual-index primers, and 12 PCR cycles. For knockout strains, library preparation and sequencing were performed by SeqCenter in Pittsburgh, PA using Illumina's Stranded Total RNA Prep Ligation with Ribo-Zero Plus kit and custom probes.