GSM8525901: Pool 1 - ADT; Mus musculus; RNA-Seq
Viable, TCRb+CD4+GFP+tdTomato+ Tregs were sorted and labelled with Biolegend TotalSeq C HTOs (TotalSeq™-C0301 anti-mouse Hashtag 1 Antibody = WT1; TotalSeq™-C0302 anti-mouse Hashtag 2 Antibody = WT2; TotalSeq™-C0303 anti-mouse Hashtag 3 Antibody = KO1; TotalSeq™-C0303 anti-mouse Hashtag 4 Antibody = KO2) and pooled (second pool has same format with WT3, WT4, KO3, KO4). Approximately 10,000 cells per batch were loaded onto the 10X Genomics Chromium Controller (Chip K). Gene expression libraries were prepared using the 10x Genomics Single Cell 5' Reagent Kits v2 (Dual Index) following manufacturer user guide (CG000330 Rev B).