GSM8977187: Control, donor 2 (Ct_D2); Homo sapiens; RNA-Seq
The CD8+ CAR-T cell population was isolated using a FACSAria III cell sorter (BD Biosciences). Total RNA was then extracted from the purified CD8+ T cells using the NucleoSpin RNA Kit (Macherey-Nagel), according to the manufacturer's instructions. RNA libraries were prepared for sequencing using the TruSeq Standard mRNA Library Prep kit according to standard Illumina protocols.