HvABI5 is an important ABA-dependent regulator of drought stress response at heading time in barley
HvABI5 is an important ABA-dependent regulator of drought stress response at heading time in barley
Tissue was collected from the middle part of the second leaf below the flag leaf in four biological replications. Each biological replication contained a fragment of leave from three independent plants. Seed were germinated on Petri dishes at 28°C in a growth chamber. After 5 days seedlings were transplanted to seedling pots and grown at 25°C. On the 20th day of growth plants were placed in 3L pots filled with a mixture of coarse sand with loam (1:3) and maintained under optimal water supply in greenhouse (22/16°C day/night, photoperiod 16/8h). 9 selected plants of each genotype were transferred to a PlantArray intelligent screening system (Plant-DiTech) on the 85th day of growth.  PlantArray lysimeter allows for strict monitoring of the water regimen and accurate drought application based on regular weight measurements and tracking of selected physiological parameters (Appiah et al. 2023). On the 99th day of growth (heading time, growth stage 50-59, Zadoks et al. 1974) 5 of the 9 plants of each genotype growing on a lysimeter were subjected to drought stress. Drought was applied for 15 days. After drought stress (flowering, growth stage 60-69, Zadoks et al. 1974) tissue collection for RNA-seq was performed. RNA was extracted using a mirVana RNA isolation kit (Thermo Fisher Scientific) according to the manufacturer's instructions. True Seq libraries were constructed for each sample. PE 150.