GSM9284641: d2_OP9_DLL1_unprimed_LSK_progeny_bulk_RNA_rep2; Mus musculus; RNA-Seq
Total RNA was isolated using RNeasy Mini Kit (Qiagen #74105) with on-column DNase I treatment (Qiagen #1023460) Bulk RNA-seq library was constructed using NEBNext Ultra II RNA Library Prep Kit for Illumina. Bulk ATAC-seq library was constructed after transposition reaction using Tn5 transposases (Illumina #15027916) by following previously published protocol (Corces et al., 2017 Nat Methods, PMID: 28846090). CUT&RUN library was constructed using NEBNext ChIP-Seq Library Preparation Kit (NEB #E7645S, #E7500S, #M5505S) by following previously reported protocol ((Liu et al., 2018 Cell, PMID: 29606353) Single-cell RNA-seq cDNA libraries were prepared using 10X Chromium 3' capture v3.1 kit. Single-cell hashtag oligo library was prepared in accordance with the BioLegend TotalseqA guide.