16S rRNA gene sequencing of Penaeus vannamei: hemolymph
The 16S rRNA gene V3V4 region was PCR-amplified using the primers 515F (5-GTGCCAGCMGCCGCGGTAA-3) and 806R (5-CCGTCAATTCMTTTRAGTTT-3). Sequencing libraries were constructed following standard amplicon library preparation protocols to generate paired-end reads (2  250 bp).