RNAseq of of Fusarium oxysporum f. sp. lactucae race 1 isolate JCP043 grown in PDB adjusted to a pH of 9 with NaOH for 2 days
RNA was extracted using TRIzol reagent (Life Technology, Karlsruhe, Germany) and purified with a Zymo RNA clean and Concentrate kit (Zymo, Irvine, CA), according to the manufacturers instructions. The quality and quantity of purified RNA were determined using the NanoDrop spectrophotometer and Agilent 4200 TapeStation. Libraries were prepared using the Watchmaker Genomics mRNA Library Preparation kit with IDT xGEN 10nt Unique Dual-Index primers following manufacturers recommendations. Completed libraries were assessed for quality and quantified using a combination of Biotium AccuGreen High Sensitivity dsDNA and Agilent 4200 TapeStation. All libraries were barcoded, normalized, and pooled equimolarly for sequencing in an AVITI Cloudbreak Freestyle High Output flow cell. Sequencing was performed in a 2  150 bp paired end format using an AVITI Cloudbreak Freestyle High Output 300 cycle kit.