GSM8645917: humanDLPFC_Schizophrenia_5; Homo sapiens; RNA-Seq
The DLPFC tissue blocks were cryo-sectioned, and total RNA was extracted from 200 mg of cortical tissue using the RNeasy Mini Kit (QIAGEN). For a subset of replicate samples, dry homogenization was performed using a liquid nitrogen cooled mortar and pestle before RNA extraction. TruSeq stranded mRNA library preparation kit (Cat# 20020595, Illumina Inc.) to prepare barcoded sequencing libraries from 300 ng of total RNA, which included polyA selection. Unique dual indices (catalog number 20022371, Illumina Inc.) were used. The library preparation was performed following the manufacturer's protocol (#1000000040498). The pooled libraries were sequenced using the NovaSeq 6000 system (Illumina Inc.) with a S4 flowcell and v1.5 sequencing chemistry, resulting in approximately 80 million reads per sample with paired-end 150 bp read length