GSM8524789: Tcells_RTT; Mus musculus; RNA-Seq
Chromium Next GEM Single Cell Fixed RNA Sample preparation kit was used according to the manufacturer's protocol. In brief, 1 x 106 cells were fixed for 22 hours at 4°C, quenched, and long-term stored at -80°C according to 10X genomic Fixation of Cells & Nuclei for Chromium Fixed RNA profiling (CG000478) using Chromium Next GEM Single Cell Fixed RNA Sample preparation kit (PN-1000414, 10X Genomics). 250,000 cells per sample were used for probe hybridization using the Chromium Fixed RNA Kit, Mouse Transcriptome, 4rxn x 4BC (PN-1000496, 10X Genomics), pooled equally and washed following the Pooled Wash Workflow as described in the Chromium Fixed RNA Profiling Reagent kit protocol (CG000527, 10X Genomics). Library was constructed according to manufacturer's protocol using the 10x Genomics 3' CellPlex multiplex kit.