16S rRNA transcript-based amplicon sequencing of semi-hard cheese microbiome: Vacherin Fribourgeois made from raw milk with the raw milk adjunct culture LI, manufactured on 2022-08-10
Amplicon libraries were prepared using the unidirectional fusion method. PCR of the V1V2 16S rRNA gene region was performed using primer NGS_ABCxF27 and NGS_trP1_355. The amplification was carried out as follows: 94 C for 2 min, followed by 18 cycles of 94 C for 30 s, 55 C for 30 s, and 68 C for 30 s. All amplicons were purified using AMPure XP beads with a bead-to-DNA ratio of 1.8.