GSM9588852: SRM IC50 replicate 2 RNA-seq; Homo sapiens; RNA-Seq
On day 7 of differentiation, cultures were lysed in QIAzol lysis reagent. Total RNA was extracted using the RNeasy MinElute Cleanup Kit (Qiagen, Cat# 330231) according to the manufacturer's instructions. For RNA-seq, 100 ng total RNA was used as input. Ribosomal RNA was depleted using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB, Cat No.: E7490). The libraries were constructed using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NEB, Cat No.: E7760).