GSM8666764: L. japonicus MYB36 line2  UV control conditions, shoots, biological replicate 2; Lotus japonicus; RNA-Seq
Tissue was flash-freezed in liquid N2, ground with mortar and pestle and RNA extracted with a modified CTAB protocol Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP.