leaf sample
Third-generation sequencing library construction and quality assessment: large fragments of DNA (greater than 15Kb) were enriched and purified using magnetic beads. This process included the repair of damaged ends and polishing of the fragmented DNA. After purification, A-tailing was performed on both ends of the DNA fragments. Sequencing adapters from the SQK-LSK109 kit were then ligated to prepare the library. The constructed DNA library's concentration was precisely measured using Qubit. Sequencing was conducted on the PromethION P48 sequencer (Oxford Nanopore Technologies, Oxford, UK) after loading the purified library onto R9.4 Spot-On Flow Cells.