GSM8587115: ChIP input, set-21(red109), rep 2; Caenorhabditis elegans; ChIP-Seq
Briefly, grind of approximately 5000 adult worms was corsslinked and then sonicated to 200-500bp.  Immunoprecipitation was performed using the following antibodies: anti-H3K23me3 (61500, Active Motif), anti-H3K9me3 (ab8898, Abcam).  The pulled-down complexes were reversed crosslinked by proteinase K digestion and then purified by phenol chloroform extraction. The yielded DNA was used to construct barcoded ChIP-seq libraries using the KAPA Hyper Pre Kit (Roche) according to the manufacturer's instruction. standard linker ligation-based DNA library