GSM8646310: con1; Mus musculus; RNA-Seq
Blood samples from anesthetized mice were collected into anticoagulant tubes and peripheral blood mononuclear cells (PBMCs) were prepared by a centrifugation method using HISTOPAQUE (Cat. 10771, Sigma-Aldrich, USA). After centrifugation, the PBMC layers were carefully harvested by a Pasteur pipette. Collected cells were further purified using a PBS solution, followed by centrifugation (250 x g, 4°C, 10 min).  Contaminant red blood cells (RBCs) were lysed using an RBC lysis buffer (Cat. 130-094-183, Miltenyi, Germany). After termination of RBC lysis with 2% FBS, purified PBMCs were collected by centrifugation Library was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics)