GSM8841635: wild-type, control MEF rep1; Mus musculus; RNA-Seq
Total RNA was extracted using TRI Reagent and ethanol precipitated. 5-10 ug of total RNA was further processed to remove the remaining DNA with TURBO DNase and column purified prior to library construction. Total RNA libraries were prepared using the TruSeq Stranded Total RNA Library Prep Gold (Illumina, 20020598) following manufacturer's protocol.