Prokaryotic Community_CSTR reactor_Cellulose_Day 52_Replicate 1
For 16S rRNA sequencing, digestate was collected again (Day 0, 10, 28 and 52), with two replicates per reactor. After bioplastics removal, samples (10 mL) were pelleted by centrifugation at 13,000 rpm for 20 minutes at 4C, and the supernatant was discarded. All samples were stored at -80C before DNA extraction. For DNA extraction, pellets (0.060 g) were extracted using a Power Fecal Pro DNA Kit (Qiagen, Germany) according to the manufacturers guidance. Purity (A260/A280 and A260/A230) of the extracted DNA was assessed by using a Nanodrop 1000 spectrophotometer (Thermo Fisher Scientific), while concentration was measured using a Qubit 1dsDNA Broad Range assay kit (Thermo Fisher Scientific). Fragmentation was determined through gel electrophoresis, 1 % (w/v) 1 TAE agarose gels. DNA was stored at -80C until analysis. The NGS was performed at Novogene Co., Ltd (Cambridge, UK). Sequencing targeted the V3 and V4 regions of the bacterial 16S rRNA gene using primers 341F (CCTAYGGGRBGCASCAG) and 806R (GGACTACNNGGGTATCTAAT) (Yu et al., 2005). The generated DNA libraries were sequenced with an Illumina NovaSeq PE250;