GSM8646320: CeD1; Homo sapiens; RNA-Seq
Iodixanol gradient centrifugation was used for single nucleus isolation. A proper amount of single nuclei (~8,000) was added to the reverse transcription mixture, single cell 3' gel beads (combined with 10x barcode), and partitioning oil in the 10x Genomics Chromium™ Single Cell Controller before starting the GEM (Gel Bead-In-EMulsions) generation program. GEM generation, reverse transcription, cDNA amplification, and library construction were performed followed by the standard protocols of 10x genomics. The library sequencing was performed in the Illumina Hiseq platform (Illumina, San Diego, CA, USA) under the 2 × 150-bp double-ended sequencing pattern to generate FastQ data.