GSM8713917: Plaque macrophages, Control, replicate 2; Mus musculus; RNA-Seq
Total RNAs were extracted from plaque macrophages using the Quick-RNA miniprep plus kit (Zymo). n = 4 biological replicates per genotype, with each replicate comprising pooled samples from 2-3 male mice, resulting a total of 10 male mice per genotype. The SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara) was used to reverse transcribe and amplify small amounts of RNA (10 ng per sample). After generating cDNA, the library was prepared using Nextera XT (Illumina).