16S amplicon sequencing of positive control metagenome: mock community consisting of an even mix of the genomic material for 20 known bacterial strains (ATCC MSA01002)
Total DNA from mock community consisting of an even mix of the genomic material for 20 known bacterial strains (ATCC MSA01002) was used as a positive control. DNA was assessed qualitatively by agarose gel electrophoresis and quantitatively using the QubitTM dsDNA Broad Range Quantitation Kit. DNA samples were standardized to 10 ng/L in UltraPure distilled water, and PCR amplification of the V4 region of 16S rRNA gene was performed as previously described. Successful amplification was confirmed by agarose gel electrophoresis and QubitTM dsDNA High Sensitivity Quantitation Kit. Individual samples were normalized using SequelPrepTM Normalization Plates, pooled, and concentrated using the Zymo Research DNA Clean and Concentrator.