HF3, Bisulfite-Seq
Genomic DNA was extracted from muscle tissue of Cyprinus carpio using a standard phenol-chloroform method. DNA was fragmented and subjected to bisulfite conversion using a commercial bisulfite conversion kit. Sequencing libraries were constructed following standard protocols for whole-genome bisulfite sequencing. The libraries were sequenced on the Illumina NovaSeq 6000 platform to generate paired-end reads.