scRNA-Seq of Spodoptera exigua hemocytes (immune-challenged)
Fifth-instar Spodoptera exigua larvae were injected with Escherichia coli, incubated for 8 h, and hemolymph was collected for single-cell preparation. Cells were captured with 10x Chromium 3 prime v2, and libraries were prepared by poly(dT) priming with cell barcodes and UMIs, followed by Illumina paired-end sequencing. This sample represents hemocyte transcriptomes following immune activation.