GSM9405362: IUE-ZR-4, replicate 2 (C08b), Tracker-seq; Mus musculus; RNA-Seq
Tumors were dissociated using the Miltenyi Brain Tumor dissociation kit. Live GFP+ cells were sorted and loaded on a 10x Chromium Chip. Libraries were constructed using the 10x Chromium Next GEM Gene Expression Kit and assessed via Agilent High Sensitivity D5000 TapeStation. They were then sent for sequencing at St. Jude's Hartwell Center on a NovaSeq 6000. Libraries had 400 million reads with a 28-10-10-90 cycle configuration.