GSM9515639: WT#3 (bulk RNA); Mus musculus; RNA-Seq
CD8⁺ T cells were sorted from lymph nodes and spleens of WT and BIT mice (9–10weeks old, male, n = 3 individual mice per group, >98% purity).Total RNA was prepared from sorted cells with the RNeasy Kit (Qiagen). The quality of RNA was assessed by Bioanalyzer (Agilent). The library was made by using the Stranded Total RNA Prep Ligation with Ribo-Zero Plus for sequencing (Illumina).