GSM8702781: HAP1,wild-type,ATAC; Homo sapiens; ATAC-seq
Adherent cells were digested with 0.25% trypsin and collected in a 15mL tube containing serum-containing medium, followed by centrifugation and washing of the cell pellet with serum-free basal medium to obtain a single-cell suspension. 1 × 10⁵ freshly cultured cells were washed with PBS, digested, and centrifuged at 500 × g for 5 min at 4°C. The cell pellet was resuspended in 400 μL lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630) and incubated on ice for 5 min. Spin down at for 500 × g for 5 min at 4°C. Then the resulting pellet was performed using the TruePrep DNA Library Prep Kit V2 (Vazyme, TD501) according to the manufacturer's instructions. Briefly, the resulting pellet were incubated with 5× TTBL, TTE Mix V50, and ddH₂O at 37°C for 30 min, followed by fragmentation. DNA fragments were purified using phenol-chloroform-isoamyl alcohol (25:24:1). Libraries were constructed by 8 cycles of PCR amplification using the TruePrep Library Prep Kit, followed by magnetic bead purification. Libraries were sequenced on a Novogene HiSeq PE150 platform. Two biological replicates were performed for each condition.