GSM8493530: NUGC-3 cells, DMSO, 5h, bio rep 1; Homo sapiens; ssRNA-seq
Total RNA was extracted immediately from the cells after treatments for 5 or 16 h as indicated and purified by using QIAcube (Qiagen) with DNase I digestion, following the manufacturer's protocols. After rRNA depletion by using Fastselect HMR kit (Qiagen), strand-specific RNA sequencing libraries were prepared by using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB), following manufacturers' instructions.