scRNA-Seq of Mus musculus: HDM-exposed wild-type lung CD45+ cells
CD45+ cells were isolated from homogenized lung tissues via FACS. Single-cell suspensions were loaded onto microwell chips using the Singleron Matrix Single Cell Processing System. Barcoding beads were subsequently collected from the microwell chips, followed by reverse transcription of the mRNA captured by the barcoding beads to obtain cDNA which were amplified via PCR. Amplified cDNA was fragmented and ligated with sequencing adapters. The scRNA-seq libraries were constructed according to the protocol of the GEXSCOPE Single Cell RNA Library Kits (Singleron).