GSM8644638: p53WT ChIP S2; Homo sapiens; ChIP-Seq
p53 ChIP-seq was performed using a SimpleChIP® Plus Enzymatic Chromatin IP Kit (9005, Cell Signalling technology) following the manufacturer's instructions. Briefly, cells were fixed and nuclei was purified as described in DNA-ChIP. DNA was then fragmented by treating with Micrococcal Nuclease for 20 min at 37°C and sonicated. Fragmented DNA was subjected to IP as described above using DO-1 p53 antibody (2mg/ml) and ChIP-Grade Protein G Magnetic Beads. Quality of the purified ChIP DNA was analyzed by Agilent 2100 Bioanalyzer according to the manufacturer's instructions and proceeded for library preparation and deep-sequencing using PE150 strategy on Ilumina platform (Novogene, Cambridge, UK).