GSM9409989: TG3 KO, pool of 2 mice, FBL; Mus musculus; OTHER
Murine HCC-liver tissues were minced into small pieces (Approx~1mm) before incubating with enzyme cocktail of mouse tumor dissociation kit (Miltenyi Biotec) for one hour at 37°C. For both human and mice tissues, each sample was then suspended in PBS + 1% BSA before loading for droplet encapsulation using the 10x Genomics Chromium Controller or iX at a targeted cell recovery of about 5,000 cells per sample. Single cell gene expression libraries were prepared using the 10x Genomics Chromium Next GEM Single Cell 3′ v3.1 Library & Gel Bead Kit according to the manufacturer's protocol. The resulting libraries were subjected to 2 × 151 cycle sequencing on an Illumina NovaSeq 6000 targeting 50,000 read-pairs/cell.