GSM9449297: RNA-Seq, iBAT from wild-type mouse #1; Mus musculus; RNA-Seq
Total RNA is extracted from  adipose tissues homogenized in TRIsure (Bioline, #BIO-38033) reagent and ISOLATE II RNA Mini kit (Bioline, #BIO-52073). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis