metagenomic of marine: Northwest Pacific Ocean S30
Genomic DNA was fregmented to approximately 400 bp using a Covaris M220 Focused-ultrasonicator (Gene Company Limited, Hong Kong, China). Dual-indexed paired-end libraries were prepared with the NEXTFLEX Rapid DNA-Seq Kit (Bioo Scientific, Austin, TX, USA) following the manufacturers instructions. Sequencing was carried out on the Illumina NovaSeq platform (Illumina, San Diego, CA, USA) using a paired-end configuration.