GSM9283607: KO4; Mus musculus; RNA-Seq
Microglial cells were fluorescently sorted by means of FACS, gating for TdTomato+/DAPI-/ RedDotTM1 Far-Red Nuclear Stain+ (Anawa, #40060), directly in RLT lysis buffer. RNA was harvested using RNAeasy micro plus Kit (Quiagen 74034) RNA DNBSEQ Eukaryotic Transcriptome was sequenced by BGI genomics