GSM8493685: s24_cortex_SSS_F3; Rattus norvegicus; miRNA-Seq
Cortical and placental tissue was collected from euthanized gestating dams on gestational day 21 via caesarean section. Fetal cortices were dissected and isolated for analysis. Genomic DNA was extracted and digested with restriction enzyme MspI, followed by blunt-ending, adapter ligation, and bisulfite conversion. RNA was isolated using TRIzol reagent and treated with DNase I. Small RNA libraries were prepared from total RNA. For bisulfite sequencing, bisulfite-converted DNA libraries were prepared by ligating methylated adapters to digested DNA fragments, followed by size selection and purification. For RNA sequencing, mRNA libraries were prepared using the Illumina TotalPrep RNA Amplification Kit, and small RNA libraries were prepared using the TruSeq small RNA library construction kit. Whole genome bisulfite sequencing (WGBS) was used for DNA methylation analysis, RNA sequencing (RNA-seq) was employed for mRNA expression analysis, and small RNA sequencing was used for miRNA expression analysis.