GSM9039088: HCT116 MD, mid-dose cell line, 5 uM oxaliplatin, 10 cycles, replicate 3; Homo sapiens; RNA-Seq
Cells were washed twice with ice-cold PBS before being scrapped and transferred to eppendorf tube. RNA was extracted using Qiagen RNA extraction kit. RNA was sent to GIS sequencing core. RNA was extracted using the RNeasy Plus Mini Kit (QIAGEN) following manufacturer's protocol. Yield and integrity of RNA were determined using the Agilent 2100 Bioanalyzer (Agilent Technologies) following manufacturer's protocol. Sample preparation for the Bioanalyzer was done using the Agilent RNA 6000 Nano Kit (Agilent Technologies, USA) following manufacturer's protocol.