GSM9628994: PBMC from COVID-19 patient 1; Homo sapiens; RNA-Seq
To isolate PBMCs, first centrifuge the anticoagulated whole blood to remove the plasma layer, then resuspend the remaining cell pellet in PBS. Carefully layer this suspension over the human lymphocyte separation medium and centrifuge with the brake off to create a density gradient. Finally, collect the white cloudy interface (the PBMC layer), treat it with RBC lysis buffer to remove contaminating red cells, and wash the resulting pellet with PBS for downstream use. Libraries were prepared using BGI/MGI standard protocols. This included mRNA enrichment, fragmentation, double-stranded cDNA synthesis, and adapter ligation for DNBSEQ sequencing.