GSM8646098: Human Coronary Smooth Muscle Cells, H3K27ac ChIP-Seq input; Homo sapiens; ChIP-Seq
Cells were crosslinked with 1% Formaldehyde for 10 min at room temperature and quenched with glycine (125mM) for 5 min. The cells were rinsed twice with cold 1XPBS and sonicated in nuclei lysis buffer. Library preparation was performed on the eluted ChIP DNA using the NEB Ultra II library preparation kit, according to the manufacturer's protocol. 10 PCR cycles were performed using indexed primers and the library of DNA fragments with sizes between 300 to 500 bp was selected using the Omega MagBind NGS cleanup Magnetic beads (Omega M1378). The libraries were sent for paired end sequencing with at least 50 million read pairs at 2 x 151 bp read length on the illumina HiSeq 4000 platform.