RNA-seq of Red clover leaf tissue
The poly(A)+ fraction was isolated from total RNA using a Dynabeads mRNA Purification Kit (Ambion). A library for sequencing was prepared using the NEBNext Ultra II RNA Library Prep (New England Biolabs) and random hexamer primers. The library concentration was measured using the Qubit dsDNA HS Assay Kit on Qubit 2.0. fluorometer (Thermo Fisher Scientific). Fragment length distribution was analyzed using an Agilent High Sensitivity DNA Kit. The library was sequenced on a HiSeq 1500 (Illumina)