GSM9515002: HFD mouse WT 2 liver; Mus musculus; RNA-Seq
Liver RNA was extracted using Trizol and Qiagen Rneasy Mini kit (74104) Total RNA samples were quality checked with Agilent Fragment Analyzer. Samples that passed quality check were used as input to generate RNA-Seq libraries using the Illumina TruSeq stranded mRNA library preparation kit following the manufacturer's instructions. The resulting libraries were subsequently quantified with Agilent Fragment Analyzer and pooled at equal molar amounts and sequenced