GSM8774233: CHIP2024_426_Wave5; Homo sapiens; OTHER
Our expanded cohort longitudinally sampled from the LBC1936 was processed as per our previous work. Briefly, DNA was extracted from whole blood with Ethylenediaminetetraacetic acid (EDTA) using the Nucleon BACC3 kit (Sigma-Aldrich, GERPN8512), according to the manufacturer's protocol. Libraries were generated from 200 ng of each DNA sample using the Invitea/Archer VariantPlex® 75 Myeloid gene panel and the VariantPlex® Somatic Protocol for Illumina sequencing (Invitae, AB0108, and the VariantPlex®-HCG Myeloid Kit; see Supplementary Table 2), with adaptations for detecting low variant allele frequencies. Sequencing was performed on the NextSeq 550 platform (Illumina) using the NextSeq 500/550 High-Output version 2.5 kit (300 cycles). Two 'Genome in a Bottle' (GIAB) DNA samples (NA12878, Coriell Institute) were included in each sequencing batch to ensure reproducibility and support background error modeling and batch correction.