ONT sequencing of CYXGZ: leaf
Size-selected long DNA fragments were processed with the Ligation Sequencing Kit SQK-LSK109 (Oxford Nanopore Technologies, UK). DNA ends were formalin-fixed and paraffin-embedded (FFPE), followed by end repair/dA-tailing using the NEBNext module (New England Biolabs, UK). Sequencing adapters were ligated with the NEBNext Quick Ligation module (New England Biolabs, UK). The final library was sequenced on a PromethION platform (R10.4.1 flow cell; Oxford Nanopore Technologies, UK) via single-molecule real-time nanopore sequencing.