ATAC-seq experiments to define the role of MEIS transcription factors in cardiac differentiation
ATAC-seq experiments to define the role of MEIS transcription factors in cardiac differentiation
Embryoid bodies were dispersed with TrypLE, cells were washed with ice-cold PBS, counted, aliquoted in 50000 to 100000 cells/aliquot and pelleted. Embryonic stem (ES) cells were cultured on a monolayer of mitomycin-inactivated mouse embryonic fibroblasts and maintained in DMEM/F12-based medium containing 20% knockout serum replacement (Invitrogen) and 10 ng/ml bFGF (Miltenyi Biotech). hPSCs were passaged using TrypLE Select (Invitrogen). HESC NKX2-5eGFP/w and MEIS1/MEIS2 KO cell lines were differentiated using the embryoid body (EB) method as described by in  https://doi.org/10.1038/nmeth.1740. Cell pellets lysis, tagmentation and DNA purification was performed using ATAC-Seq Kit (Active Motif) following manufacturer`s instructions. Purified DNA was amplified with 10 PCR cycles using Nextera indexes following ATAC-Seq Kit (Active Motif) protocol.