GSM8879442: Synovium, End-stage Ankle Osteoarthritis, Sample 20; Homo sapiens; RNA-Seq
Following operative removal of the tissue, samples were immediately flash-frozen in liquid nitrogen and stored at -80ºC. RNA was extracted using TRIzol™ following the manufacturer's suggested protocol (ThermoFisher) and purified (GeneJET RNA Purification Kit). Messenger RNA was purified using poly-T oligo-attached magnetic beads. After fragmentation, first-strand cDNA was synthesized using random hexamer primers, followed by second-strand cDNA synthesis with dUTP for a directional library or dTTP for a non-directional library. Libraries were quantified using Qubit and real-time PCR, while size distribution was assessed with a Bioanalyzer.