GSM9359236: Circulating cells from day0,1,5 after VSV infection; Mus musculus; RNA-Seq
Approximately 150 µl of blood was collected from the tail artery into a tube containing 100 µl of 50 mM EDTA-2K in PBS before, 1 day and 5 days after the infection. The red blood cells were lysed with ACK lysing buffer (Thermo, A1049201). Cells were fixed with CellCover (Anacyte, 800-125) at 4℃ for 15min to preserve RNA. Cells were then resuspended in CELLBANKER 1 (Nippon Zenyaku Kogyo Co., Ltd., 11910) and stored at -80℃. Samples from the survival (n=2) and lethal (n=2) groups were processed for subsequent library preparation following mRNA Whole Transcriptome Analysis (WTA) and Sample Tag Library Preparation Protocol from BD Rhapsody™ Single-Cell Analysis System.