Nanopore whole genome sequencing of Pasteurella multocida
The isolate was streaked out on a blood agar plate. A single colony was inoculated into Nutrient Broth and grown at 37C. High molecular weight genomic DNA was prepared using the Qiagen Tip 100 Kit according to the manufacturer's protocol. Genomic DNA libraries were prepared using the rapid sequencing (SQK-RAD004) kit, loaded in a FLO-MIN106 (R.9.4.1) flow cell, and sequenced using a MinION system, with live super-accurate base calling (Dorado). Hybrid assembly of Illumina and ONT reads was carried out using Unicycler.