GSM9509264: Whole Genome Methylseq, HSEC Donor1, PCSK9-EE7000, 3; Homo sapiens; OTHER
Genomic DNA was extracted from PHHs, HSECs, and HAdCCs using a split lysate protocol to extract both RNA and DNA from the same sample lysate (Beckman Coulter). Genomic DNA was mechanically sheared on a Covaris R230 Focused Ultrasonicator to a size range of 350 – 450 bp. Sequencing libraries were then constructed from the sheared genomic DNA using the NEBNext Enzymatic Methyl-seq (EM-seq) kit plus NEBNext Multiplex Oligos for Enzymatic Methyl-seq. Libraries were then sequenced using an Illumina NovaSeq X instrument with 2 x 151 bp reads to a read-depth of >1 billion read pairs per sample.