Ancestor D39 isolate RNA replicate 3
RNA was sequenced by Vertis-Biotech as follows, rRNA depletion was carried out from total RNA using the Ribo-Zero rRNA Removal Kit (Bacteria) (Illumina) followed by NGS library preparation of strand specific cDNA using adapter ligation to 3'OH ends of fragmented RNA. Finally, Illumina NextSeq 500 sequencing was used to produce single reads of 1x75 bp read length and about 1x10 M reads.