GSM9283729: BV-2 cells, SF188 exposed, 3h, N1; Mus musculus; RNA-Seq
Total RNA was isolated using the RNeasy Plus Mini kit (Qiagen) with an additional step of Dnase treatment. The yield and quality of the isolated RNA were analysed using NanoDrop spectrophotometer. Total RNA samples were subjected to quality control with Agilent Tapestation according to the manufacturer's instructions. The 200 ng of total RNA was subjected to Illumina sequencing and libraries were prepared with the Illumina TruSeq Stranded mRNA kit which includes cDNA synthesis, ligation of adaptors and amplification of indexed libraries. The yield and quality of the amplified libraries was analysed using Qubit by Thermo Fisher and the Agilent Tapestation. The indexed cDNA libraries were normalized and combined, and the pools were sequenced on the Illumina Hiseq 2000 generating 50 bp single-end reads.