NGS of Escherichia coli KPC-2 rectal swab
Whole genome sequencing libraries were prepared using the Illumina DNA Prep kit following the manufacturer's instructions. Briefly genomic DNA was fragmented and tagged with adapters via bead-linked transposomes (BLT). Libraries were amplified using Enhanced PCR Mix (EPM) and indexed with Illumina DNA CD Indexes. Sequencing was performed on an Illumina iSeq 100 system using 2x150 bp paired-end reads.