PBDE debrominating enrichment culture Genome sequencing and assembl
The library preparation for metagenomic sequencing using NovaSeq PE250  begins with the extraction of high-quality genomic DNA from the sample. After assessing DNA concentration and integrity, the DNA is fragmented into smaller pieces (~350 bp) using mechanical shearing. The fragmented DNA undergoes end-repair and A-tailing to prepare the ends for adapter ligation. Sequencing adapters, along with barcodes, are then ligated to the DNA fragments. Size selection is performed to retain the desired fragment size, followed by a limited PCR amplification to enrich the library. After quality control to assess fragment distribution, the library is loaded onto the NovaSeq flow cell for paired-end 250 bp sequencing (PE250). The NovaSeq platform provides high-throughput sequencing, generating raw FASTQ files, which are subsequently used for downstream bioinformatics analysis to explore microbial diversity and functional potential.