GSM9514234: CD34+ HSPC derived erythroblasts;KMT2D;CUT&RUN;Rep2; Homo sapiens; OTHER
10 μl concanavalin beads were added to the samples, followed with gentle rotation for 10 min at room temperature. Beads were resuspended in 100 μl buffer (Dig-wash buffer with 0.05% digitonin ) with 2 μg KMT2D antibody. On ice, 2 μl 100 mM CaCl2 was added to the sample with gentle mixing.rred to a new tube.  Afterward, samples were incubated on ice for 60-180 min. Then 100 μl stop buffer was added to the samples and samples were incubated for 10 min at 37°C followed by centrifugation at 4°C, 12,000 rpm. DNA was extracted by the phenol/chloroform/isopropanol protocol. Library construction was performed using the DNA Library Prep Kit.