ITS amplicon seq of cultivable SS AMD bacteria
Fungal ITS2 amplicon sequencing library  was prepared using DNA, extracted from the cultivated SS abandoned mine drainage sample with ZymoBIOMICS DNA Kit, as a template and 2024F: 5-CATCGATGAAGAACGCAGC-3 and 2409R: 5-TCCTCCGCTTATTGATATGC-3 barcoded primer pair, targeting Internal Transcribed Spacer 2 (ITS2) hypervariable region, located  between 5.8S and 28S rRNA genes. Sequencing was performed by Novogene sequencing provider on the Illumina platform to generate 250 bp paired-end raw reads, 30K tags per sample, by Novogene sequencing provider.