GSM8743909: MOG_Ctrl_OL_2; Mus musculus; RNA-Seq
Brain and spinal cord cell suspensions were prepared, and oligodendrocytes, astrocytes, and microglia were isolated using magnetic-activated cell sorting (MACS) with cell type-specific MicroBeads: oligodendrocyte: Anti-O4 MicroBeads, astrocyte: Anti-ACSA-2 (astrocyte cell surface antigen-2) MicroBeads, and microglia: Anti-CD11b MicroBeads. RNA extraction was performed using the RNeasy Plus Micro Kit following the manufacturer's protocol. RNA quality was assessed using the Bioanalyzer 2100 system (Agilent Technologies), and all RNA samples had a RNA integrity number (RIN) greater than 6.8. The cDNA library was prepared using the NEBNext Ultra Directional RNA Library Prep Kit (New England BioLabs).