GSM8524561: pronephric duct, sibling in CRISPR/Cas9 mutant lines, 72hpf, rep4; Danio rerio; RNA-Seq
RNA was harvested according to the following step. Prepared the lysis solution of the following system(0.1ul RRI; 0.19ul 10% triton x 100; 2.41ul nuclear free water; 1 ul dNTP(10mM) ), placed the pronephros ducts in it, and stored it frozen in liquid nitrogen . The extracted total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using KAPA Hyper Prep Kits following manufacturer's protocols.