GSM8775063: DPSC, vasculogenic diff., day7; Homo sapiens; RNA-Seq
Virto sample; Cells were collected with trypsin and washed with PBS. Vivo sample; Implants were retrieved and digested in a solution of 3 mg/mL collagenase type I and 4 mg/mL dispase for 40 min at 37°C. DPSC-GFP were sorted by flow sorter. Collected cells were suspended in PBS with 0.04%BSA. The concentotation was 1000cells/µl. Single cell suspensions were subjected to final cell counting on the Luna Fx7 Automated Cell Counter (LogosBio) and diluted to a concentration of 700 -1000 cells/ul. 3' single cell libraries were generated using the 10x Genomics Chromium Controller and following the manufacturer's protocol for 3' V3.1 chemistry with NextGEM Chip G reagents (10x Genomics). Final library quality was assessed using the LabChip GXII HT (PerkinElmer) and libraries were quantified by Qubit (ThermoFisher). Pooled libraries were subjected to 150 bp paired-end sequencing according to the manufacturer's protocol (Illumina NovaSeq 6000).