scRNA-seq of Timer Blue+red- CD69+TCRb+ thymocytes from pooled Nr4a3-Tocky mice
CD69+TCRb+ thymocytes from 3 pooled Nr4a3-Tocky thymi flow sorted using FACS Aria III. Droplet single-cell RNA-seq libraries constructed using Chromium Next Gem Single Cell 3' Kit v3.1 (10x Genomics); R1=Barcode+UMI, R2=cDNA insert, I1=Sample index.