GSM9284743: F22_KD; Canis lupus familiaris; RNA-Seq
The cells (3 × 10^4) were seeded on 36-mm dishes and subjected to siRNA transfection. Eighteen hours after the second transfection, RNA was extracted from the cells using the miRNeasy Mini Kit (QIAGEN, 217004), according to the manufacturer's instructions. The cDNA libraries were constructed according to the manufacturer's instructions using the TruSeq Stranded mRNA Library Prep (Illumina, Inc., San Diego, CA).