GSM8587053: MSI2, RRM1 RBNS, 250, rep2; synthetic construct; RNA-Seq
Bound RNA was eluted from streptavidin magnetic beads in 0.1% SDS with 0.3 mg/mL proteinase K. RNA was concentrated via phenol-chloroform extraction. RNA libraries for sequencing were reverse transcribed with SuperScript IV following manufacturer's protocols, PCR amplified with Phusion DNA polymerase to attach Illumina TrueSeq Small RNA adapters, then agarose gel purified.