GSM8840783: RUES02, primed (E8), H3K4me3 ChIPseq, replicate 001; Homo sapiens; ChIP-Seq
Cells were gently collected using a mixture 1:1 Versene/Accutase (5 min) and pass through a 70um cell strainer. Cells were fixed with freshly prepared 11% formaldehyde, 5M NaCl, 0.5M EDTA ph8.0, 1M HEPES ph7.9 for 15 minutes at room temperature. After quenching  with 1/20 volume pf 2.5M glycine solution), cells were washed with PBS, 0.5% Igepal. The protease inhibitor PMSF  was added (1mM) prior to preparing snapfrozen cell pellets for Active Motif's FactorPath and HistonePath services. 40 ug chromatin was used as starting material for input and ChIP samples (4-10uL antibody per ChIP).