RNA-Seq of homo sapien vastus lateralis : adult male day 14 limb immobilised
Briefly, 600ng of each RNA sample was used for library construction using the TruSeq Stranded Total RNA Library kit with Ribo-Zero Gold rRNA depletion (Illumina) as per manufacturers instructions. Adapter-ligated fragments were amplified by PCR for 11 cycles and the quality and size of the final library preparations analysed on a TapeStation (Agilent). Indexed samples were pooled and then sequenced on a NextSeq 500 system (Illumina) across 8 flow cells, generating approximately 62 million paired-end 2 x 75bp reads per sample. Following sequencing the data was demultiplexed and the resulting FASTQ files used for bioinformatics analysis.