GSM9359968: Spinal cord nuclei, Sedentary and Trained, Timepoints PRE and 6h; Mus musculus; RNA-Seq
Spinal cords were dissected and snap-frozen in liquid nitrogen. Nuclei were isolated from frozen, lumar regions of the tissue in lysis buffer using a dounce-based homogenizer. The suspension was filtered before fixation for 1 h at room temperature with the 10X Genomics Fixation Kit. Fixed nuclei were stained for DAPI to enable FACS-based separation of single nuclei from debris and duplets. Sorted nuclei were cryopreserved until sequencing library preparation. SnRNA-Seq libraries were generated with the Chromium Next GEM Fixed RNA Kit according to the manufacturer's instructions (10X Genomics, User Guide Rev D, CG000522). 16 samples were multiplexed per run.