GSM8667572: DN1_RNA_ER_TcfLefKO_r1; Mus musculus; RNA-Seq
RNA-seq and ATAC-seq were performed on the sort-purified Lin-CD8-CD4-CD44hiCD25- ckit+ cells. The cells were used for total RNA extraction using standard Qiagen RNeasy columns, or treated with TN5 transposase to generate tagged DNA fragments. RNA libraries were prepared for sequencing using stardard Illumina protocol. TN5-tagged DNA fragements were purified, PCR amplified to build ATAC-seq libraries.