Amplicon sequencing of Influenza A barcoded virus from tissue
Viral RNA extraction was performed using the MN Kit, followed by a one-step RT-PCR reaction using the SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity DNA Polymerase and primers targeting the barcoded region of our virus. Samples were purified using AMPure XP Beads, then quantified using the Qubit dsDNA quantification kit prior to paired end sequencing.