16S rRNA-seq of mus musculus: adult male feces (chow)
An index PCR was performed to attach dual barcodes and Illumina sequencing adapters using Nextera XT Index kit (Illumina). Final PCR products were verified on 1.5% DNA agarose gel and quantified using Pico dsDNA assay (Invitrogen). An equal molar of each sample was combined and purified again using Ampure XP beads as the library