RNAseq of deep-sea shrimp: eye
Libraries were constructed from high-quality RNA using the NEBNext UltraTM II Directional library prep kit for Illumina using the manufacturers protocol for use with the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490). Libraries had a target amplicon size of 330 bp and contained NEBNext Multiplex Oligos for Illumina dual index adaptors. Purification of cDNA was done using AMPure XP beads. Libraries were pooled and sequenced on an Illumina HiSeq4000 to obtain 150 bp paired-end reads.