GSM9039141: Macrophages without LPS treatment, Actd 3h; Mus musculus; RNA-Seq
Cells were washed twice with cold PBS. 500 μl lysis buffer (8 mM HEPES, pH 7.4, 80 mM KCl, 4 mM MgCl2, 2% Triton x-100, and 200 μg/ml cycloheximide) was then added, and the cells were quickly scraped and collected into RNase-free tubes. The sample were centrifuged at 12,000 rpm for 12 minutes at 4°C, and the supernatant was sent to Novogene (China) for RNA-seq library construction. Approximately 100 μl of supernatant were treated with RNase I (Thermo Fisher Scientific) for 3 hours, followed by RNA extraction using TRIzol™ LS. RNA samples were sent to Novogene for RNA-seq library construction