GSM8773272: GATA2 low HUDEP-2, lentiviral empty vector, IgG isotype control (HA), replicate 2; Homo sapiens; OTHER
Following tagmentation, samples were treated with Proteinase K and incubated at 37C overnight.  DNA was purified by spin clolumn (Cell Signaling Technology, #14209) Libraries were prepared with the NEB Next HiFi 2x PCR Master Mix (M0514S) following the manufacturer's protocol using 16 cycles of amplification. Sample concentration was determined using Qubit 1X dsDNA HS Assay (ThermoFisher Q33231). The quality of the library was assessed using Bioanalyzer High Sensitivity DNA Analysis (Agilent).