RNA-seq of CD8+ cells: mus musculus spleen
CD4+CD19B220 and CD8+CD19B220 T-cells (1x105) isolated from murine spleen and sorted using a FACSAria (Figure S2) into Eppendorf tubes containing 2% FBS in PBS were centrifuged at 350 RCF for 10 minutes. RNA was extracted following the MagMAXTM-96 Total RNA Isolation Kit protocol (Thermo Fisher, AM1830). The RNA was then sent to DKFZ for ultra-low input library preparation and sequencing using NovaSeq 6K PE 50 SP.