GSM9266872: 2M TDP Ctrl; Mus musculus; RNA-Seq
To collect the fresh tissues for snRNA-seq, mice were euthanized in CO2 chamber and mice brains were immediately dissected out. Using a vibratome, mice brains were freshly sliced into 200-400 µm thickness and the mPFC region was dissected out utilizing fine forceps under microscope. Four to six mPFC tissues (40-60 mg) were combined and immediately snap-frozen in liquid nitrogen and shipped to SingulOmics with dry ice for snRNA-sequencing. Nuclei were isolated and 3' single cell gene expression libraries(Next GEM v3.1) were constructed using 10x Chromium system. The libraries were sequenced with 200 million PE 150 reads per sample in Illumina NovaSeq.