GSM9191622: Human CKD plasma EVs sample hp25; Homo sapiens; miRNA-Seq
Plasma was isolated from blood collected in sodium citrate tubes and sequentially centrifuged to remove cells, platelets, and microvesicles. Extracellular vesicles (EVs) were isolated via ultracentrifugation (120,000 × g, 90 min), and total RNA was extracted using the miRNeasy micro kit (Qiagen). Small RNA libraries were prepared using the SMARTer smRNA-seq Kit (Takara Bio). Small RNA sequencing libraries were selected using AMPure beads for corrected size.