WGS of Klebsiella pneumoniae
Libraries were prepared using the SQK-LSK110 Ligation Sequencing kit in conjunction with the PCR-Free ONT EXP-NBD104 and EXP-NBD114 Native Barcode Expansion kit and performed without optional shearing steps to select for long reads. Individual libraries were quantitated through Qubit 4.0. At last, the library was sequenced on the nanopore minION platform.