KPC_V (CRX1193486)
Total RNA was extracted using Trizol reagent (Thermo Fisher, 15596018) following the manufacturer's procedure. The mRNA was fragmented into short fragments using divalent cations under elevated temperature (Magnesium RNA Fragmentation Module (NEB, cat. E6150, USA) under 94 degrees Celsius for 5-7 minutes). The cleaved RNA fragments were then reverse-transcribed to create the cDNA using SuperScript II Reverse Transcriptase. Finally, we performed 2x150 bp paired-end sequencing (PE150) on an Illumina NovaSeq 6000 (LC-Bio Technology CO., Ltd., Hangzhou, China) following the vendor's recommended protocol.