GSM8612402: E14.5GFP&RFP_K2; Mus musculus; ATAC-seq
After FACS, cells were washed twice in ice-cold PBS, and 50,000 cells (per sample) were used to perform ATAC-Seq. Cells were resuspended in 50 μl ice-cold ATAC Lysis Buffer , and centrifuged at 500 g at 4 °C to isolate nuclear pellets Nuclear pellets were treated with Nextera Tn5 Transposase (Illumina, FC-121-1030) in a 50 μl reaction for 30 min at 37 °C.  Transposed DNA was immediately isolated using a Qiagen MinElute PCR Purification Kit, and then PCR amplified in a 50 μl reaction using a common forward primer and different reverse primers with unique barcodes for each sample