GSM8587160: PBS 1d_3; Mus musculus; RNA-Seq
On day 1 after PBS/LPS injection or day 7 after LPS injection, mice were anesthetized and sacrificed, and fresh PVT tissues were collected. Total RNA was extracted using the Trizol Reagent. The concentration, quality, and integrity of the extracted RNA were evaluated using a NanoDrop spectrophotometer (Thermo Scientific, Wilmington, DE, USA). Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA, USA) following manufacturer's protocols.