GSM8957791: rheb1;head;Lpp[TS]>Rheb;rep1; Drosophila melanogaster; RNA-Seq
Total RNA was extracted from heads of females for RNA-seq analysis. Fly heads were dissected in ice-cold PBS, transferred into RNAiso Plus (TaKaRa #9109), and homogenized with the Precellys Evolution Homogenizer (Bertin Technologies). 20 heads were used for each sample in biological triplicates. Total RNA extraction was performed as previously described 84. RNA concentration was measured by Nanodrop One (Thermo Fisher Scientific). RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies). Then, the sequencing libraries were constructed using VAHTS Universal V10 RNA-seq Library Prep Kit (Vazyme) and sequenced on an Illumina Novaseq 6000 platform with 150 bp paired-end reads.