Human K562 ATAC-cFOOT rep2
ATAC-cFOOT-seq involves Tn5 transposase tagmentation on open chromatin region of genome followed by enzymatic deamination, subsequent library preparation utilized the Illumina V3 EpiArt DNA methylation kit, incorporating primers designed to match the adapter sequences integrated by Tn5 transposase.In brief, single-stranded DNA was ligated with adapter sequences at the 3' end, followed by initial amplification using universal 3' primers and primers specific to the Tn5 transposase adapter sequences. This step involves introducing universal adapters compatible with the Illumina next-generation sequencing platform at the 5' end of the Tn5 adapters via overlap PCR. Finally, universal primers were used for the ultimate library amplification.