GSM9282524: HepG2 RNA-seq, siControl, replicate 3; Homo sapiens; RNA-Seq
Total RNA was isolated using the Quick-RNA Miniprep Kit (Zymo Research) according to the manufacturer's instructions [with on-column DNase treatment]. RNA quantity was measured by spectrophotometry/fluorometry, and integrity was assessed (Bioanalyzer/TapeStation); only high-quality RNA was used for library prep. mRNA was enriched by poly(A) selection [or: rRNA depletion—select one], fragmented, and converted to cDNA. Sequencing adapters were ligated and libraries PCR-amplified per the manufacturer's instructions (kit: [e.g., Illumina TruSeq Stranded mRNA]). Libraries were pooled and sequenced as paired-end reads on an Illumina [model; e.g., NovaSeq/NextSeq] platform with [e.g., 2×75 bp or 2×100 bp] chemistry.