GSM8775409: brown adipose tissue, Male PGC-1α knockout, room temperature(25℃); Mus musculus; RNA-Seq
Total RNA was isolated using the RNeasy Plus Universal Mini Kit (Qiagen). Sequencing libraries were prepared using the NEBNext Ultra™ RNA Library Prep Kit for Illumina (New England Biolabs, USA), following the manufacturer's instructions. The libraries were sequenced on an Illumina NovaSeq 6000 platform, generating 150-bp paired-end reads.