GSM8977169: NFD cells treated with TGFb1 sample 2; Homo sapiens; miRNA-Seq
RNA was isolated from 625μL sEVs pretreated with 1mg/mL Proteinase K for 10 min at 56°C, followed by treatment with 4μg/mL of RNase A for 1h at 37°C. Samples were processed using the mirVana™ PARIS™ RNA and Native Protein Purification Kit according to the manufacturer's protocol for Total RNA (ThermoFisher). 50µL total RNA isolated from sEVs was cleaned up using the RNA Clean & Concentrator™ kit (Zymo) according to manufacturer's protocol, where small RNA (17-200nt) was further concentrated for RNA sequencing. NEBNext Small RNA Library Prep Set for Illumin