saccharomyces cerevisiae MU2R48 variation Raw sequence reads
After the DNA samples were received, the samples were assayed;  The DNA samples qualified by electrophoresis detection were randomly interrupted into fragments of about 350bp in length by Covaris ultrasonic crusher. After the DNA fragments were processed, the whole library preparation was completed by end repair, addition of A-tail, addition of sequencing junction, purification, PCR amplification and other steps;  After the library construction was completed, Qubit2.0 was used for preliminary quantification, and the library was diluted to 2ng/ul. Subsequently, the insert size of the library was detected using Agilent 2100, and after the insert size met the expectation, the effective concentration of the library was accurately quantified using the Q-PCR method to ensure the quality of the library;  After passing the library inspection, different libraries were pooled according to the effective concentration and the target downstream data volume for IlluminaHiSeq sequencing.