GSM9626401: C. portucalensis, cultured in tryptic soy broth, rep1; Citrobacter portucalensis; RNA-Seq
Total RNA was extracted using the Qiagen RNeasy Mini Kit. Ribosomal RNA was depleted from total RNA, followed by ethanol precipitation and fragmentation. First-strand cDNA was synthesized using random hexamer primers, and second-strand cDNA synthesis was performed using dUTPs to allow strand specificity. The libraries were then prepared through end repair, A-tailing, adapter ligation, size selection, PCR amplification, and purification. Library quality and concentration were assessed using Qubit fluorometry, real-time PCR, and an Agilent Bioanalyzer.