GSM8743521: LA1589_BioRep2_6dpi; Solanum pimpinellifolium; RNA-Seq
RNA was extracted using TRIzol reagent (Invitrogen™,15596018) according to manufacturer's instruction. 100ng total RNA per sample was used for RNA library construction with kits from NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB, E7490) and NEBNext® Ultra™ II RNA Library Prep with Sample Purification Beads (NEB #E7775) following the provided manuals, excepting for using our own adaptor and primers. The adapter was synthesized duplex oligoes of iTrusR1stub: ACACTCTTTCCCTACACGACGCTCTTCCGATCT and iTrusR2-stubRCp: /5phos/GATCGGAAGAGCACACGTCTGAACTCCAGTCAC.