GSM8772879: AMO-1 shNTC Mock Rep2; Homo sapiens; RNA-Seq
RNA from 1x10^6 cell was first extracted with RNeasy Plus Mini kit (Qiagen), per manufacturer's protocol. Total RNA was quantified with Qubit RNA HS Assay Kit (Thermo Fisher Scientific) and quality was assessed using RNA ScreenTape on 4200 TapeStation (Agilent Technologies). Truseq Stranded mRNA kit (Illumina) was used with an input of 100 nanograms of total RNA. Libraries were quantified with Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific) and the average library size was determined using D1000 ScreenTape on 4200 TapeStation (Agilent Technologies). Libraries were pooled and sequenced on HISeq 1000 (Illumina) to generate 30 million single-end 50-base pair reads for each sample.