GSM8745088: 5-day-old chicks, Mock, rep 2; Gallus gallus; RNA-Seq
RNA was harvested using Trizol reagent(Invitrogen, CA, USA). Total RNA was extracted using the Trizol reagent. RNA purity and quantification were evaluated using the NanoDrop 2000 spectrophotometer (Thermo Scientific, USA). RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Ribo-off rRNA Depletion Kit（vazyme, Nanjing, China）was used to remove ribosomal RNA, then the libraries were constructed using VAHTS Universal V6 RNA-seq Library Prep Kit according to the manufacturer's instructions.