16S rRNA gene amplicon sequencing of Bacteria: heated water sample
Total genomic DNA extractions from the heated water sample from the shale oil were carried out using AxyPrepTM Bacterial Genomic DNA Miniprep Kit (Axygen Biosciences, CA, USA). The bacterial 16S rRNA genes were PCR amplified by primer sets 515F: 5-GTGCCAGCMGCCGCGGTAA -3 and 806R: 5-GGACTACHVGGGTWTCTAAT-3, respectively.