ITS2 sequencing of Galaxea fascicularis Symbiodiniaceae  community
DNA was extracted from adult coral samples. The extraction was conducted with bead beating and additional enzymatic digestion steps with Proteinase K. Symbiodiniaceae ITS2 primers SYM_VAR_5.8S2 (forward: 5-GTGACCTATGAACTCAGGAGTCGAATTGCAGAACTCCGTGAACC-3) and SYM_VAR_REV (reverse: 5-CTGAGACTTGCACATCGCAGCCGGGTTCWCTTGTYTGACTTCATGC-3) (Hume et al., 2018) with Illumina adapter sequences (underlined) added at the 5-prime ends were used to amplify the ITS2 molecular marker.