GSM9512232: MASH; Mus musculus; RNA-Seq
Single cells were isolated after Percoll gradient centrifugation of nonparenchymal cells (NPC) isolated from perfused livers. In brief, the livers were perfused with Collagenase D, hyaluronidase, and DNases I in DMEM media. NPC was harvested after the removal of hepatocytes at 50 g. Percoll gradient centrifugation was performed in 26.25%, 41.5% and 73.3% percoll gradient layers according to a published report. In Sc-RNA seq, libraries were constructed according to the manufacturer's manual (Flex Gene Expression GEM-X kit, 10XGenomics). Briefly, fixed single cells were processed and barcoded using the GEM-X platform, and sequencing libraries were constructed and amplified according to standard 10x Genomics procedures. Fixed single cells were processed and barcoded using the GEM-X platform, and sequencing libraries were constructed and amplified according to standard 10x Genomics procedures.