GSM8646101: Human Coronary Endothelial Cells, H3K27ac HiChIP; Homo sapiens; OTHER
At least one million cells from the same lot used for HiChIP experiments were crosslinked with 1% Formaldehyde for 10 min at room temperature and quenched with glycine (125mM) for 5 min. The protocol was followed using Arima High Coverage Hi-C Kit (Arima Genomics, A410110), as per manufacturer's instructions. Library was prepared using NEB Ultra II Library preparation kit (NEB, E7645L) according to manufacturer's protocol and appropriate index primers. 12 PCR cycles were performed using indexed primers, and DNA fragments were cleaned up with Omega MagBind NGS Cleanup Magnetic Beads. Libraries were sequenced by paired-end sequencing with at least 300 million read pairs at 2 X 151 bp read length on illumina HiSeq4000 platforms.