Whole genome resequencing of marine medaka: muscle
The DNA fragments underwent end polishing and 3' acetylation, followed by additional PCR amplification. Subsequently, the constructed libraries underwent comprehensive quality and yield assessment using the Agilent 2100 Bioanalyzer and ABI StepOnePlus Real-Time PCR System. The libraries were then sequenced using the Illumina HiSeq platform, generating 150bp paired-end reads with an insert size of approximately 350bp.