GSM8742896: FSTD8_3; Felis catus; RNA-Seq
RNA was extracted using the Direct-zol RNA Miniprep kit (Zymo Research) according to the manufacturer's modified protocol for “RNA purification from the aqueous phase after Tri Reagent extraction”. Tumor samples were crushed in liquid nitrogen, mixed with 500µL Trizol, and further lysed using a bead mill. Chloroform (100 µL) was added to the lysate, mixed well, and the mixture subjected to centrifugation. The aqueous phase was removed, mixed with an equal volume of 100% ethanol and added to a Zymo-Spin IICR column. After centrifugation and a quick wash, on-column DNA digestion was performed, followed by additional washes. Total RNA was eluted with 50 µL of RNAse/DNAse-free water and 1 µg was submitted to Novogene Co. for quality testing, library prep, and sequencing.