16S rRNA sequencing of Gallus gallus: cecal microbiome from commercial broilers
Cecal content samples from broiler chickens (Gallus gallus) were flash-frozen in liquid nitrogen. Genomic DNA was extracted using TianGen magnetic bead kit, followed by PCR amplification of 16S rRNA V4 region with primers 515F/806R. Libraries were prepared with NEB Next Ultra II FS Kit and sequenced on Illumina NovaSeq 6000 (PE250). Bioinformatics: Raw reads processed by fastp (QC), FLASH (merging), DADA2 (ASV generation), and SILVA 138.1 (taxonomy). Subsequent analyses performed in QIIME2.