RNA-Seq of digestate: condition3
DNA was extracted using the QIAGEN PowerSoil DNA kit. Amplicon fragments were generated from the extracted DNA using high-fidelity Phusion Plus polymerase, targeting the full length of the 16S gene (regions V1-V9). The 16S genes were then amplified and the pooled amplicons were sequenced on the PacBio Sequel 2 platform using new 8M chips.