NSCLC primary tumor, STK11 c.358G>T p.E120*, KRAS WT (low-pass shallow whole genome sequencing)
Single cell and single nuclei TCR libraries were prepared from amplified cDNA libraries according to manufacturer instructions using the following reagents (all 10X Genomics): Chromium Single Cell V(D)J Enrichment Kit for human T cells (#1000005) was used for cDNA generated with Chromium Single Cell V(D)J reagents (#1000006), and final sequencing libraries were prepared using Chromium i7 multiplexing kit (#120262). Single Cell Human TCR Amplification Kit (#1000252) was used for cDNA generated with Chromium Next GEM Single Cell 5' V2 reagents (#1000263), and final sequencing libraries were prepared using Library construction kit (#1000190) and Dual Index Kit TT set A (#1000215).