Soil microbial community under NCM exposure
Soil DNA was extracted from sieved soil samples using the E.Z.N.A. Soil DNA Kit (Omega Bio-tek, USA) following the manufacturers protocols. The fungal internal transcribed spacer (ITS) region was amplified by PCR using sample-specific barcoded primers. PCR reactions were performed in triplicate, and the resulting amplicons were purified, quantified, and pooled in equimolar concentrations. Amplicon libraries were constructed using a PCR-based approach and sequenced on an Illumina MiSeq platform with paired-end reads (2  300 bp). Sequencing was conducted by Shanghai Biozeron Biotechnology Co., Ltd.