RNA-Seq. of alpine soil C20-6
Total DNA was extracted (DNeasy PowerSoil Kit, Qiagen) from 0.25 g of soil. PCR amplified the V3-V4 region of the bacterial 16S rRNA gene using primers 341F and 805R (Herlemann et al., 2011; Klindworth et al., 2013). PCR products were purified, indexed (Nextera XT Index Kit), pooled, and sequenced on an Illumina MiSeq platform