metagenome of mus musculus: cecal content
Bacterial genomic DNA was extracted by Guangdong Magigene Biotechnology Co.,Ltd. (Guangzhou, China) using commercial kits. DNA integrity and purity were monitored on 1% agarose gels. DNA concentration and purity were measured using Qubit 3.0 (Thermo Fisher Scientific, Waltham, USA) and Nanodrop One (Thermo Fisher Scientific, Waltham, USA). Sequencing libraries were generated using ALFA-SEQ DNA Library Prep Kit. The library quality was assessed on the Qubit 4.0 Fluorometer (Life Technologies, Grand Island, NY) and Qsep400 High-Throughput Nucleic Acid Protein Analysis system (Houze Biological Technology Co, Hangzhou, China).