scRNA-Seq of Spodoptera exigua hemocytes (naive)
Fifth-instar Spodoptera exigua larvae were injected with PBS were collected, incubated for 8 h, and hemolymph was collected. hemocytes filtered into single-cell suspension, and processed with 10x Chromium 3 prime v2 workflow. Libraries were generated by poly(dT) priming to capture poly-adenylated mRNA with cell barcodes and UMIs, followed by Illumina paired-end sequencing. This sample represents baseline hemocyte transcriptomes.