DNA-seq F3
DNA was and DNA quality and concentration were examined. Amplicon libraries targeting the bacterial 16S rRNA gene variable regions 1-8 were prepared. Forward and reverse primers used in the PCR included custom 24 nt barcode sequences followed by the sequences targeting bV18A: [8F] AGRGTTYGATYMTGGCTCAG and [1391R] GACGGGCGGTGWGTRCA. The amplicon libraries were purified. Sequencing libraries were prepared from the purified amplicon. DNA was used as input. The final sequencing library was loaded onto a PromethION R10.4.1 flowcell and sequenced using MinKNOW 25.05.12. Reads were basecalled and demultiplexed using the basecalling algorithm and custom barcodes.