GSM8879666: mouse E14 brain, control, rep1; Mus musculus; RNA-Seq
RNA was harvested using Rneasy mini plus kit (QIAGEN). Total RNA was used for the construction of sequencing libraries. rRNA depletion was performed with the Ribo-Zero rRNA Removal Kit (Illumina, San Diego, CA, USA). The enriched mRNA was randomly fragmented using fragmentation buffer, and cDNA synthesis was carried out using the NEB Next Ultra RNA Library Kit (Illumina) according to the manufacturer's protocol.