GSM8646406: cDC2_1; Homo sapiens; RNA-Seq
Total RNAs were extracted from each sample using TRIzol Reagent (Invitrogen, cat. NO 15596026). RNA-seq libraries were prepared by KC-DigitalTM Stranded mRNA-seq Library Prep Kit for Illumina® (Catalog NO. DR08502, Wuhan Seqhealth Co., Ltd. China) following the manufacturer's instruction. The library products corresponding to 200-500 bps were enriched, quantified, and finally sequenced on a Novaseq 6000 sequencer (Illumina) with a PE150 mode.