GSM8741260: Shotgun sequencing of WT after O/N culture, rep 3; Saccharomyces cerevisiae; OTHER
After the O/N incubation, a 1 mL aliquot from each culture was collected for subsequent shotgun sequencing. DNA was extracted using zymolyase, proteinase K and phenol and processed with Collibri kit. Preparation of the samples for paired-end sequencing on an Illumina NextSeq500 (2x35 bp) was performed using Invitrogen TM Collibri TM PS DNA Library Prep Kit for Illumina and following manufacturer instructions.