Assembling a kanamycin resistant version of strain Pla107
A single colony of strain DBL328 (a kanamycin resistant isolate of strain DAB885, which itself is an isolate of Pla107 in which plasmid pMTN1907 has been recombined into the megaplasmid) was picked to King's B media and grown overnight while shaking at 27 degrees C. A Promega Wizard Kit (Madison, WI) was used to isolate genomic DNA, which was then shipped to Plasmidsaurus for sequencing with their standard pipeline using 10.4 flowcells.