GSM8612406: Knee_Creb5RFP_Creb5_2; Mus musculus; OTHER
Cut&Run-Seq (Cleavage Under Targets and Release Using Nuclease) was performed using a CUTANA™ ChIC/CUT&RUN Kit (Epicypher, Cat#: 14-1048) according to the manufacturer's protocol The sequencing libraries were prepared using a NEBNext® UltraTM II DNA Library Prep Kit (New England Biolabs, Cat#: E7645L). PCR products in the libraries that were bigger than 350 bp and smaller than 150 bp were removed by two rounds of selection using AMPure beads (Beckman Coulter