GSM8773168: YO-F1; Bos grunniens; OTHER
Grind the frozen tissue into powder using a mortar and pestle in liquid nitrogen, and then dissolve it in 400 μ L lysis buffer. The ribosomal profiling technique was carried out according to the report. Add short (50-80 base) antisense DNA probes complementary to rRNA sequences to a solution containing RFs, and then add RNase H and DNase I to digest rRNA and residual DNA probes. Finally, further purification of RFs was carried out using magnetic beads. Add adapters to both ends of RFs and perform reverse transcription and PCR amplification. Enrich PCR products with sizes of 140-160bp to generate cDNA libraries, and sequence using Illumina HiSeqTM X10.