Nanopore long reads of Opsariichthys evolans
Genomic DNA (5~8 g) for Nanopore sequencing was end-repaired and ligated via a KAPA Hyper Prep Kit (Cat#KR0961, Kapa Biosystems, Wilmington, MA, USA), following the manufacturers instructions. The genomic DNA library was premixed with the LB and SQB buffer in the Nanopore Ligation Sequencing Kit (SQK-LSK109, Oxford Nanopore Technologies, UK) loaded in a flow cell (R9.4.1; FLO-MIN106), and sequenced by MinION devices for 24-72 h.