GSM9267491: Hashtags, lane A; Mus musculus; OTHER
B16-gB melanoma tumours were excised, minced, and digested in Liberase TL research grade solution at 0.25 mg per ml for 60 min at 37 °C. Cell suspensions were triturated, passed through 70 µm strainers, and erythrocytes were removed with eBioscience red cell lysis buffer. Cells were Cells were cryopreserved in 10% DMSO: 90% FCS and stored in liquid nitrogen. Viable DAPI negative transgenic CD8 T cells (OT I or gBT I) were stained and sorted via fluorescence activated cell sorting, then stained with hashtag antibodies (Biolegend) according to manufacturer's protocol. Libraries were made according to the 10x Genomics protocol (Chromium Single Cell 5' v2)