GSM9191870: batch35-BN-control-6weeks; Mus musculus; RNA-Seq
Following at least 12 hours of seizure-freedom on EEG, pilocarpine-treated and control littermates were processed in batches. Mice were anesthetized with isofluorane and decapitated. Brains were quickly removed and bilateral hippocampi were dissected on ice. Each hippocampus was bisected and the dorsal half was snap frozen and stored at -80 C for nuclei isolation at a later date. Nuclei were isolated from frozen tissue by Dounce homogenization in 0.1% NP-40 and purification on a discontinuous iodixanol gradient. Single-cell libraries were prepared using the Chromium Single Cell 3' Reagent Kit v3 (10x Genomics) according to the manufacturer's protocol.