CITE-seq of 52 matched primary AML samples from diagnosis and relapse
CITE-seq of 52 matched primary AML samples from diagnosis and relapse
Bone marrow mononuclear cells (BMMCs) were isolated by standard Ficoll purification methods and viably frozen from bone marrow biopsies obtained from patients with AML. Cells were loaded at a concentration of 18,000 for a targeted cell capture of 10,000 cells using the Chromium Next GEM Automated Single Cell 5' Kit v2 and Chromium Next GEM Chip K Automated Single Cell Kit (10X Genomics). Cells were loaded at a concentration of 18,000 for a targeted cell capture of 10,000 cells using the Chromium Next GEM Automated Single Cell 5' Kit v2 and Chromium Next GEM Chip K Automated Single Cell Kit (10X Genomics). Single-cell emulsions were then prepared for sequencing according to the manufacturer's protocol (10X Genomics)