GSM9588866: pThr4 ChIP-seq, SCP1 D96A mutant treatment, biological replicate 2; Homo sapiens; ChIP-Seq
Cells were fixed by 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a Biorupter UCD-200, and then anti-pThr4 antibody (6D7) was used for immunoprecipitation (IP). ChIP-seq libraries were prepared using NEBNext Ultra II DNA Library Kit for Illumina and sequenced as paired-end 150-bp reads on a Illumina NovaSeq 6000