Modulation of liver immune-metabolic pathway in hybrid grouper infected with Edwardsiella ictaluri
Modulation of liver immune-metabolic pathway in hybrid grouper infected with Edwardsiella ictaluri
Before the start of the experiment, 600 healthy hybrid groupers with an average body weight of 42.3 ± 2.8 g were acclimatized in a recirculating aquaculture system (water temperature: 28–30°C; pH: 7.8 ± 0.2) and fasted for 24 h. The fish were then randomly allocated to two treatment groups: Control group ( con)which received no treatment; E. ictaluri-infected group (ADH) which was administered E. ictaluri via oral gavage.  Total RNA was extracted from the midgut of  hybrid groupers by using the TRlzol Reagent and following the manufacturer's instructions (Life Technologies, California, USA). cDNA libraries were constructed as per the instruction manual of NEBNext Ultra RNA Library Prep Kit.