GSM9449358: FOLFOX18; Mus musculus; RNA-Seq
Subcutaneous tumors were harvested from tumor-bearing mice and processed into single-cell suspensions. Approximately 12,000 cells per sample were targeted for capture using the 10X Genomics Chromium Single Cell 3' Kit (v3) on a 10X Chromium chip. Library preparation and sequencing were conducted by LC-Bio Technology Co., Ltd. (Hangzhou, China) on an Illumina NovaSeq 6000 platform, employing a paired-end 150 bp sequencing strategy to achieve a minimum sequencing depth of 20,000 reads per cell.