RNA-seq reads of Lindera aggregata
Total RNAs were isolated from the young leaves, fruits, stems, flower buds, taproots and jointed tuberous roots of the same plant. The concentration of RNAs were assessed by Nanodrop and Agilent 2100. The integrity and purity of RNAs were assessed by agarose gel electrophoresis and Agilent 2100. After the RNA samples were tested, mRNA was enriched with magnetic beads with Oligo (dT). Subsequently, fragmentation buffer was added to break the mRNA into short fragments. Using the mRNA as a template, one-strand cDNA was synthesized using six-base random hexamers. Then buffer, dNTPs, and DNA polymerase I and RNase H were added to synthesize two-strand cDNA. The double-strand cDNA was then purified using AMPure XP beads. The purified double-stranded cDNA was first end-repaired, A-poly, and connected to sequencing adapters, and then fragment size was selected using AMPure XP beads. PCR amplification was then carried out and the PCR products were purified using AMPure XP beads to obtain the final sequencing library. Sequencing was performed via the Illumina NovaSeq X plus platform.