GSM9266930: Tg, sIL10ra-4; Mus musculus; RNA-Seq
RNA was extracted from frozen, pulverized forebrains using TRIzol reagent (Invitrogen) followed by an RNeasy mini column (Qiagen) with an on-column DNase treatment. RNA sequencing libraries were contructed using Illumina's TruSeq RNA Library Prep kit version 2; libraries were sequenced on paired-end, 100-bp runs on a NextSeq 2000 using a pooled strategy to minimize batch effects.