GSM9407366: Nfatc3-MKI_H3k27ac; Mus musculus; ChIP-Seq
Cells (1 × 10⁷) were cross-linked with 1% formaldehyde at 37°C for 10 minutes, and the reaction was quenched with 125 mmol/L glycine for 5 minutes. Nuclei were isolated using lysis buffer and sonicated using a Bioruptor UCD-300 (Diagenode) for 30 minutes (30 s ON/OFF cycle) to generate chromatin fragments ranging from 200 to 500 bp. Sequencing libraries were constructed using the NEBNext® Ultra DNA Library Prep Kit for Illumina®, including end‑repair, A‑tailing, adapter ligation, size selection (200–500 bp), and PCR amplification.