T7 Metagenome seq of AD sludge
A total of 77 samples, including inocula (6 DS and 3 FWL samples), a representative subset of treatment groups at Day 0 (CK and each type of the undissociated fatty acid), as well as the CK and all undissociated fatty acid treatment groups at days 7, 28, and 35, were subjected to shotgun metagenomic sequencing conducted by Majorbio Bio-Pharm Technology Co., Ltd. (Shanghai, China). Briefly, total DNA was extracted using the Soil DNA Kit (Omega, USA) according to the manufacturers instructions.