RNA-Seq of flu strain: nasopharynx swab
Viral RNA was extracted from a nasopharyngeal swab sample collected from a patient with signs of acute respiratory infection using the QIAamp viral RNA minikit (Qiagen, Germany) according to the manufacturers manual. Library preparation was conducted using the MGIEasy PCR-Free FS DNA Library Prep Set (MGI, China) according to the manufacturers protocol with preliminary viral cDNA synthesis (5). Sequencing was performed on an MGI DNBSEQ-G400 instrument using the MGI DNBSEQ-G400 Sequencing Set (MGI, China).