GSM8878389: BAP_24h_2; Arabidopsis thaliana; RNA-Seq
Total RNA was extracted with the GeneJet Plant RNA purification mini kit (Thermo Scientific). RNA concentrations were measured with NanoDrop 1000 Spectrophotometer and an aliquot was used for RNA quality analysis with Bioanalyzer 2100 (Agilent Technologies). DNase treatment with Heat&Run gDNA removal kit (ArcticZymes) and ribosomal RNA removal with Plant Ribo-Zero rRNA Removal Kit (Illumina) were done prior to the strand-specific library preparation with a TruSeq Stranded Total RNA Library prep kit (Illumina).