gSeq bac granules
Genomic DNA extraction using DNeasy 96 PowerSoil Pro QIAcube HT Kit. Sequencing library of 16S/18S rRNA gene variable regions 4-8 (abeV48-A) using PCR with custom barcoded primers 515FB and 1319R. Amplicon libraries purification using CleanNGS SPRI beads. The final sequencing library was prepared using SQK-LSK114 kit (ONT minIon.