RNA-Seq of Homo sapiens: decidua
Total RNA was extracted from decidual tissues and its quality was assessed using a NanoDrop spectrophotometer and an Agilent 2100 Bioanalyzer. Ribosomal RNA (rRNA) was depleted from the total RNA using a specialized kit. Following rRNA removal, the remaining RNA was randomly fragmented. First-strand cDNA was synthesized using reverse transcriptase and random primers, followed by second-strand cDNA synthesis. The resulting double-stranded cDNA was then subjected to end-repair, A-tailing, and ligation with sequencing adapters. Finally, the ligated products were PCR amplified and purified to create the final strand-specific cDNA library. The library's quality was confirmed using an Agilent 2100 Bioanalyzer, and it was subsequently sequenced on NovaSeq 6000 to generate 150 bp paired-end reads.