GSM9284773: Ezrin_Immunoprecipitation_rep2; Mus musculus; RIP-Seq
Magna RIP (Millipore, #17-700) according to the manufacturer's instructions. Normal mouse IgG (Millipore, #CS200621) and 𝛼-Ezrin (Sigma-Aldrich, #E8897) were used for the immunoprecipitation. rRNA was depleted with NEBNext rRNA Depletion Kit v2 (NEB, #E7400S) according to the manufacturer's instructions The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina platforms, according to effective library concentration and data amount required. RNA libraries were sequenced for 6 G of raw data per sample with PE 150bp read length on NovaSeq platform