GSM8956301: SmoM2-Ptenfl/fl replicate2; Mus musculus; RNA-Seq
Tumor tissues were dissected out in ice-cold Hank's balanced salt solution (HBSS) on ice, dissociated in Papain (Worthington, LK003176) at 37°C for 30 min. Following dissociation, samples were triturated by pipetting up and down using a P1000, filtered through a 40um cell strainer (Falcon), followed by PBS wash, and then centrifuged at 500â€…g at 4°C for 5min. To obtain live cells, cell suspentions were loaded to EasySep Dead Cell Removal kit (STEMCELL technologies,17899) before pool loading to 10x Chips. Cells were captured in droplets. Following reverse transcription and cell barcoding in droplets, emulsions were broken and cDNA purified using Dynabeads MyOne SILANE followed by PCR amplification per manual instruction. 10x library (3' scRNA-seq)