GSM8877850: 41879_WK13; Macaca mulatta; RNA-Seq
BAL was performed with 80 mL of sterile saline in BSL3 on NHPs at weeks 5, 11, 13 and necropsy after Mtb infection. The BAL was filtered, centrifuged (1,200 rpm, 10 min at 4C), lysed for red blood cells (RBCs) using ACK lysis buffer (GibcoTM) and resuspended in 2 mL final volume of RPMI 1640 media (GibcoTM). The cells were counted and stored in internally threaded cryovials in liquid nitrogen for downstream scRNA-seq processing using 10x Genomics platform. Library was performed according to the manufacter's instructions (single cell 3' v3.1 protocol, 10x Genomics). Target about 5-10K cells per sample.