RNA-seq of liver transcriptome of  UCP1+ cell-ablated mice at room temperature and upon acute cold exposure
RNA-seq of liver transcriptome of  UCP1+ cell-ablated mice at room temperature and upon acute cold exposure
Mice were sacrificed and livers were rapidly dissected. To induce ablation of UCP1+ cells, 6–7-week-old UCP1-DTR mice received repeated intraperitoneal injections of diphtheria toxin (DT; Sigma, 126 ng per injection). 3 DT injections were administered on days 1–3, followed by continued DT administration three times per week (8 injections in total). Control mice received saline injections according to the same schedule. One day after the final injection, mice were either sacrificed at room temperature (CTRL_RT and ABL_RT) or subjected to 3 hours of acute cold exposure at 6°C prior to sacrifice (CTRL_CE and ABL_CE). Total RNA was extracted using the RNeasy Mini Kit (Qiagen, #74104). RNA quantification was performed using a Qubit fluorometer (ThermoFisher Scientific) and RNA integrity assessed using a Bioanalyzer (Agilent Technologies). The Illumina Stranded mRNA Prep, Ligation kit was used for the library preparation with 500 ng of total RNA as input. The molarity and quality of the libraries were assessed using Qubit and Tapestation (DNA High sensitivity chip).