GSM9594062: transplanted hBOs at 150dpt after Target neuromuduation; Homo sapiens; RNA-Seq
Single-nuclei suspensions were prepared from snap-frozen hBOs grafts collected at 150 dpt. Grafts were retrieved from liquid nitrogen, placed on dry ice, and further finely dissected using a stereomicroscope. Grafts were then disrupted using a detergent-mechanical cell lysis method. Crude nuclei were filtered through a 40 μm strainer, centrifuged, and purified via sucrose density gradient centrifugation. Purified nuclei were resuspended in 0.04% BSA/PBS containing an RNase inhibitor. Approximately 18,000 nuclei per sample were loaded onto the Chromium Single Cell 3' Chip (10× Genomics, USA), and libraries were constructed using the v3 reagent kit. Pooled libraries were sequenced on an Illumina NovaSeq S4 platform (Admera Health, USA).