GSM9629024: WT control replicate 1; Bacillus subtilis; RNA-Seq
Total RNA extraction and rRNA depletion were performed by Eurofins Genomics. rRNA was removed from total RNA to enrich for mRNA. RNA concentration minimum requirement was 135 ng/ul for bacterial samples. Strand-specific cDNA library preparation was performed by Eurofins Genomics. mRNA was fragmented prior to strand-specific cDNA synthesis. Libraries were prepared following the Illumina standard for 2x150 bp paired-end sequencing using a minimum of 20 ng rRNA-depleted mRNA (>1.4 ng/ul). Paired-end sequencing (2x150 bp) was performed on an Illumina NovaSeq X, guaranteeing a minimum of 10 million reads (+/- 3%) per sample.