Sequence of HCV isolate QC510_GT 5
cDNA was generated using an oligo dA primer (not oligo dT). A TSO containing a randomized 8 bp UMI=N8 (TSO Sequence: TGAGACATCTAGCACATGTNNNNNNNNWWrGrGrG) was used to append a 5' primer sequence and Nextera XT Tn5 recognition sequence. Long PCR amplification was performed followed by Nextera XT (Illumina) processing to attach dual unique indexes and size the library to ~250 bp.