GSM9149307: Zymomonas mobilis WT Anaerobic plus Hydorgen Peroxide RNA-seq Replicate B; Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821; RNA-Seq
The RNA was extracted, the same day as the cells were harvest, following standard hot phenol:chlorofom protocol, and ethanol precipitated overnight at -80º C. The RNA then was resuspended in RNase free water and treated with DNAse I at 37ºC for 1 hour and then clean using Monarch® Spin RNA Cleanup Kit (T20240L, NEB) rRNA in the samples was depleted using the QIAseq FastSelect 5S/16S/23S, rRNA Plant, rRNA Yeast and custom rRNA Algae depletion Kits (Qiagen, Hilden, Germany). RNA-seq library preparation and sequencing was performed by the Joint Genome Institute (JGI) using default parameters. rRNA in the samples was depleted using the QIAseq FastSelect kit (Qiagen, Hilden, Germany). Libraries were constructed using the TruSeq stranded mRNA kit (Illumina) following standard JGI protocols.