R_pacifica_illumina
Library preparation was performed using QIASeq FX DNA Library Preparation protocol (Qiagen, Cat #180475).Briefly, 100 ng of Qubit ((Invitrogen, #Q32854) quantified DNA was enzymatically fragmented, end-repaired and A-tailed in a one-tube reaction using the FX Enzyme Mix provided in the QIASeq FX DNA kit.Finally, the amplified library was purified using JetSeq Magnetic Beads (Bio, # 68031). Further,the Illumina-compatible sequencing library was quantified by Qubit fluorometer (Thermo FisherScientific, MA, USA) and and the fragment size distribution was analyzed on Agilent TapeStation(Agilent, #5067-5585).The libraries were paired-end sequenced on illumina HiSeq X Ten sequencer (illumina, San Diego, USA) for 150 cycles following manufacturers instructions.