GSM8665982: mm202C_RNA_SNDX_2uM_PF9363_500nM_72h_REP2_240805_GAGTGG_S11; Mus musculus; RNA-Seq
For RNA isolation from cell lines, 1x10^6 cells were lysed in 350 µl of RLT buffer and RNA was isolated using the RNeasy Mini Kit (Qiagen) according to manufacturer's instructions for cell line experiments. 1 ug RNA was used as the input quantity for library construction. Poly-A tail selection and library preparation were performed using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA).