GSM8665969: mm202C_ChIP_MLL1_DMSO_72h_REP1_240904_AGTCAA_S13; Mus musculus; ChIP-Seq
Cells were crosslinked using 1% methanol-free paraformaldehyde for 10 min at room temperature. Glycine (25 mM) was used to quench the reaction. Nuclei were extracted and chromatin was subjected to sonication using an E220 focused ultrasonicator (Covaris). 1-5 µg of DNA was used in preparation of Illumina-compatible libraries using a ThruPLEX DNA-Seq Kit (Takara) followed by sequencing using NextSeq550 (Illumina) to obtain 20-30e6 unique sequencing paired-end reads.