GSM8586992: AML12 Mock Input; Mus musculus; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes at room temperature, quenched and lysed. Chromatin was sonicated using a Covaris M220 ultrasonicator Libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit using NEB Multiplex Oligos for Illumina Set 1 Primers. 10 ng DNA was used as starting material. 10 cycles of enrichment were used for amplification.