GSM9405375: PBS_gex; Mus musculus; RNA-Seq
Lung tissue digestion and single cell suspension preparation were performed as previously described (doi:10.1016/j.ajt.2025.08.021). Two lungs were pooled from each group at day 14 post-transplant. Samples were submitted to the Washington University Genome Technology Access Center for single nuclear genome-scale metabolic model and library construction. Library preparation was conducted with Chromium GEM-X Single Cell 3' Reagent Kits v4 (10x Genomics). Libraries were sequenced on NovaSeq 6000.