GSM9589649: Male WT E18.5 Spine Replicate 1; Mus musculus; RNA-Seq
Frozen spines were crushed using a mortar and pestle and then homogenised in 1 mL of TRIzol (Thermo Fisher) by passing through a dounce homogenizer to ensure all tissue was broken down. RNA extraction was performed on homogenised samples using a standard TRIzol RNA extraction protocol Illumina TruSeq Stranded mRNA kit