V4 16S rRNA MiSeq of extracted reactor fluid (CHER-2) gDNA
The gDNA was extracted using a FastDNA SPIN Kit for Soil (MP Biomedicals Germany GmbH, Germany) following the manual of the kit. Metataxonomic sequencing was conducted on basis of the V4 region of the 16S rRNA gene by using the 515F primer by Parada et al. and 806R by Apprill et al.