High throughput targeted 16S rRNA gene sequencing from fecal fermentations of stool of five healthy adults with seven dietary fibers.
The V4 region of the 16S rRNA gene was PCR amplified in triplicate with primers F515 and R806. Amplicons were verified by gel electrophoresis then combined, purified, and sent to the UC Davis Genome Center for library preparation and high throughput 250 bp paired end sequencing with an Illumina MiSeq.