GSM9512250: FLANK, no protein, replicate 3; synthetic construct; OTHER
DNA was purified from the electrophoresis gel using electroelution and isopropanol purification Approximately 1 ng of extracted DNA was used in a low cycle number (12-14) PCR reaction (NEBNext Hi Fidelity 2X Mastermix) with Illumina sequencing adapters. Size selection was then performed using 2.5X AMPure XP beads (Beckman Coulter). The quality of samples was measured using the Agilent BioAnalyzer and concentration was measured using the HS dsDNA Qubit assay (Thermo Fisher Scientific).