GSM9038997: OTX2_KO_H3K27me3; Homo sapiens; OTHER
Fresh cells were collected and washed with 500 μl wash buffer and centrifuged at 500×g for 5 min at room temperature. Cell pellets were resuspended with 100 μl wash buffer. The CUT&Tag assay was performed according to the manual of the Hyperactive Universal CUT&Tag Assay Kit for Illumina (TD904, Vazyme Biotech). 1 × 105 cells were used as starting material for every sample. Libraries were amplified using 12 cycles on the thermocysler. Post amplification libraries were size select at 250-450bp in length using DNA Clean Beads  (N411, Vazyme Biotech). Libraries were validated using the dsDNA HS Assay Kit (EQ121, Vazyme Biotech).