RNA-seq of Kandelia obovata from Futian County, China: eight days of post-chilling recovery at 25 degrees celcius
To identify differences in chilling induced gene expression between K. obovata and K. candel, we generated RNAseq reads for 8 to 12 biological replicates per species from the initial 25C treatment (25C initial), the 15C treatment (15C), the 10C treatment (10C), two days of post-chilling recovery at 25C (25CR1), and 8 days of post-chilling recovery at 25C (25CR2). Specifically, a single leaf from 4 to 6 individuals per population of each species was harvested during each treatment, then flash frozen in liquid nitrogen, and stored at -80C until further processing (Figure 1b). RNA extraction, library construction and sequencing were performed by Novogene (Beijing, China) using a Tiangen RNAprep Pure Plant Kit (Tiangen, Beijing, China), an NEBNext Ultra RNA Library Prep Kit  (NEB, Beverly, MA, USA), and sequenced using the Illumina HiSeq2500 platform (San Diego, California, USA), according to the manufacturers specifications. Sequencing length, 150 bp.