Comparison of biochemical, microbial and mucosal mRNA expression in bile acid diarrhoea and irritable bowel syndrome with diarrhoea
An aliquot from the stool sample was stored at -80C for subsequent microbiome analysis. Starting with 100-250 mg aliquot of stool, DNA was isolated using the DNeasy PowerSoil Pro HTP kit (Qiagen Inc., Germantown, MD, USA). Sample quality was assessed by MiSeq Nano. v2 Chemistry, library prep made using  reaction Illumina Nextera XT, and paired end sequenced using the NovaSeq S4. 2x150 PE (Illumina, San Diego, CA) applying 97 samples per flowcell. DNA isolation and sequencing were performed at the University of Minnesota Genomics Center (UMGC), Minneapolis, MN.