RNA-Seq of Paracoccus denitrificans Acetaminophen
Cells were harvested after 22 h of cultivation and immediately flash-frozen in liquid nitrogen. Total RNA was isolated using the MiniBEST Universal RNA Extraction Kit (Takara, Japan) according to the manufacturers protocol. Library construction and high-throughput sequencing were performed by Shanghai Majorbio Bio-pharm Technology Co., Ltd. (Shanghai, China), using the Illumina sequencing platform.