GSM9267894: Human spinal cord donor H25 central canal Vderived cell culture; Homo sapiens; RNA-Seq
Total RNA was isolated from NSPC cultures using a column-based kit with on-column DNase digestion (e.g., Qiagen RNeasy Plus Mini with RNase-Free DNase). RNA integrity was assessed by Bioanalyzer/TapeStation (RIN ≥ 7) and quantity by Qubit/Nanodrop. mRNA was enriched by poly(A) selection, and strand-specific libraries were prepared using the Illumina TruSeq Stranded mRNA workflow with unique sample barcodes, following the manufacturer's instructions. Libraries were sequenced on an Illumina NovaSeq 6000 (150-bp reads; single-end)