Poly(A) site profiling of the Arabidopsis oxt6 mutant
Short read sequencing libraries that query the mRNA-poly(A) junction (PAT-Seq libraries) were prepared as described previously (Pati et al., Methods Mol Biol. 2015;1255:159-74).  Briefly, RNA was fragmented, poly(A)-enriched, and used as a template for reverse transcription using anchored oligo-dT primers carrying adapter sequences to permit strand-switching, PCR amplification, and sequencing on the Illumina platform. PCR products were size selected on agarose gels, amplified, and used for sequencing.