GSM9512129: MU-1; Mus musculus; RNA-Seq
For mRNA library preparation, 1 µg of total RNA per sample was used. The mRNA was then fragmented, followed by cDNA synthesis and purification. The purified cDNA was subjected to end repair, A-tailing, and adapter ligation. cDNA fragments of 200-300 bp were selected, PCR-amplified, and purified to generate the final library. Library quality was assessed using the Agilent 4200 TapeStation. Sequencing was carried out on an Illumina NovaSeq platform with PE150 configuration.