GSM9590361: Human femoral head, osteoarthritis, donor10; Homo sapiens; RNA-Seq
Femoral head bone tissues were mechanically minced and enzymatically digested to generate single-cell suspensions. Cells were filtered through a 40 μm cell strainer and washed with PBS containing 0.04% BSA before library preparation. Single-cell RNA-seq libraries were generated using the 10x Genomics Chromium Single Cell 3′ platform according to the manufacturer's protocol. Single cells were encapsulated into droplets with barcoded gel beads, followed by reverse transcription, cDNA amplification, and library construction.