GSM9514065: FRH-CAM; Homo sapiens; RNA-Seq
For spatial transcriptomics: Fresh frozen tissue were OCT embedded, and 10µm frozen sections were cut with cryostat. For single-cell and nuclei transcriptomics: samples were freshly dissociated using custom protocol. Nuclei were isolated using 10X Genomics nuclei isolation kit according to manufacturers recommended protocol. For spatial transcriptomics: Spatial transcriptome sequencing libraries were prepared with 10xGenomics Visium Spatial Gene Expression Slides & Reagent kit according to manufacturer's construction. For single-cell an nuclei transcriptomics: single cell or nuclei suspensions were prepared and processed using the Chromium Single Cell 5' Reagent Kits v3.1