GSM8645809: mAID-RNaseh2a (the parent cell line of Pole1-M630F cells), rep 1; Mus musculus; OTHER
gDNA was prepared using the QIAGEN Blood & Cell Culture DNA Kits (20/G) Up to 25 μg of genomic DNA was treated in 0.3 M NaOH at 55 °C for 2 hrs, then loaded onto a 1.5% agarose gel and run for 2 hrs at 135 V. The gel was stained with acridine orange (final concentration 5 μg/ml) for 2 hrs at room temperature with gentle shaking followed by overnight destaining in water. Fragments of 300–2000 bp were excised from the gel and isolated with NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel #740609.250). Library preparation was performed as previously described in Daigaku et al Nat Struct Mol Biol 2015 (doi:10.1038/nsmb.2962).