GSM8615679: A549 cells, transfection reagent only; Homo sapiens; RNA-Seq
Total RNA was extracted using Trizol following the manufacturer's instructions. Total RNA obtained from each sample was subjected to a sequencing library construction using TruSeq Stranded mRNA Library Prep Kit (Illumina, Inc., San Diego, CA) according to the manufacturer's protocols. The equally pooled libraries of the samples were sequenced using NovaSeq 6000 (Illumina, Inc.) in 101-base-pair (bp) paired-end reads.