Ampl-Seq of cDNA in treated II-18 with DMSO
Amplicons of target mRNA were prepared with amino modifier C6-primers. After the second PCR for tagging of barcode sequence at the end of strand, SMRTbell libraries was prepared using barcoded universal primers for multiplexing amplicons following to PacBio protocol. DNA sequence of full-length of  target mRNA was analyzed by HiFi-mode of Sequel IIe System (PacBio) using Sequel II binding kit 2.1 (#101-820-500; PacBio). HiFi reads were computationally prepared using SMRT Link (v12.0.0.177059) for treatment of reads with circular consensus sequencing.