GSM8773488: Total RNA, Siwi Kd, rep2; Bombyx mori; RNA-Seq
Total RNAs were isolated using ISOGEN II (FUJIFILM Wako Pure Chemical) and treated with DNase (Life Technologies) followed by phenol/chloroform treatment, and precipitated with ethanol. RNA libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) with the Ribo-Zero Plus rRNA Depletion Kit (Human/Mouse/Rat) (Illumina) and sequenced on the NovaSeq platform (Illumina) to obtain paired-end reads.