microbial denove
The genomic DNA was extracted by using the Cetyltrimethyl AmmoniumBromide (CTAB) method with minor modification, and then the DNA concentration, quality andintegrity were determined by using a Qubit Flurometer (Invitrogen, USA) and a NanoDropSpectrophotometer (Thermo Scientific, USA). Sequencing libraries were generated using the TruSeq DNA Sample Preparation Kit (Illumina, USA) and the Template Prep Kit (Pacific Biosciences, USA).The genome sequencing was then performed by Personal BiotechnologyCompany (Shanghai, China) by using the Nanopore PromrthION48 platform/(Pacific Biosciences platform) and the Illumina Novaseq platform