GSM9267586: ADO (0mM),rep1; Homo sapiens; RNA-Seq
Total RNA was extracted from the sample using the TRIzol™ Reagent, followed by RNA purity with Nano Drop, RNA concentration with Qubit RNA BR (Broad-Range) Assay Kit, and RNA integrity with RNA Screen Tape. Based on the total amount of total RNA, 0.1-1ug of total RNA was taken and the mRNA was isolated using the Albertec Poly(A) mRNA Capture Module (Cat. No. RK20340) Kit, and then the mRNA library was constructed with the Arbotec Fast RNA-seq Lib Prep Kit V2 (Cat. No. RK20306) Library Building Kit.