GSM8524395: ME49, Bradyzoite, biol rep 2; Toxoplasma gondii; RNA-Seq
Intracellular parasites were harvested, released by needle passage, and purified by filtration through membranes with 3 μm pore size to remove host cell debris. Total RNA was extracted from purified parasites using transZol Up reagent (TransGen Biotech, China) following the manufacturer's instructions. The RNA quality was assessed using an Agilent 2100 Bioanalyzer (Agilent, USA). Libraries for RNA sequencing were prepared using the TruSeq RNA Sample Prep Kit (Illumina, USA) and sequenced on a DNBSEQ-T7 sequencer (MGI Tech Co., Ltd, China) with the PE150 model.