Shotgun metagenome of Drosophila melanogaster gut microbiome  Day 60, Parkin uninduced
This study aimed to investigate the impact of Parkin gene overexpression on the gut microbiome of Drosophila melanogaster across different ages. Adult flies overexpressing Parkin (ParkinOE) and age-matched wild-type (WT) controls were collected at two time points: Day 10 (young) and Day 60 (aged). For each condition, gut tissues from 100 individual flies were dissected under sterile conditions and pooled to obtain sufficient microbial DNA. DNA was extracted from each pooled sample and subjected to whole-genome shotgun (WGS) sequencing. The experimental design enables comparison of microbiome composition and functional potential between genotypes and age groups, to assess whether Parkin overexpression influences microbial dynamics related to aging and host physiology.