GSM9039325: U87 cells, compound 1, 2; Homo sapiens; RNA-Seq
Total RNA was extracted using DNA/RNA/Protein extraction kit (IBI Scientific, Dubuque Iowa) and Dnase-treated using Dnase I (RNase-free, New England Biolabs, Ipswich, MA) and further purified using RNA clean and concentrator kit (Zymo Research, Irvine, CA). Three independent biological replicates were prepared for each treatment group. A next generation sequencing library was prepared for each sample using NEBNext Poly(A) mRNA Magnet Isolation Module followed by NEBNext Ultra II RNA Library Kit for Illumina. Libraries were barcoded for multiplexing with NEBNext Multiplex Oligos for Illumina. Each multiplexed library was sequenced as a paired-end, 50 base pair sequencing run on an Illumina NovaSeq 6000, located in the Translational Science Laboratory at FSU College of Medicine