No RNA polymerase in vitro reactions with DNA oligonucleotide AF-EG-65 input 0h timepoint
Samples were generated using in vitro reactions with no polymerase control with a 27nt DNA oligonucleotide as input. This sample was the 0 minute timepoint control. Samples were purified using phenol:chloroform extraction and ethanol precipitation. The resulting purified nucleic acid was then prepared into libraries using DruSeq, a derivitized Illumina TruSeq Small RNA protocol using in-house adapters and RT primers that includes addition of polyethylene glycol in the adapter ligation steps. Reverse transcription was performed using SuperScript II. Libraries were amplified using 16 rounds of PCR and gel purified using Qiagen gel purification kit according to manufacturer's instructions. These data were NOT adapter-trimmed prior to upload.