16S_DNA_Gut_HiLo
Platform: iSeq 100; Paired  end: Yes; Read length (bp) : 301; Primer pair: V4: 515F/806R ; DNA polymerase : KAPA HiFi HotStart ReadyMix ;PCR cycle No. : 25; Adapters:Nextera XT;Sequencing depth  (avg reads per sample): 317345; Library prep and sequencing protocol: Nextera XT DNA Library Preparation Kit with the sequencing cartridge  (iSeq 100 Reagents v2)