GSM9267615: U1 rep1; Saccharomyces cerevisiae S288C; RNA-Seq
The optical density (OD) of each sample was measured to ensure an extraction efficiency yielding approximately ≥20 ng/µL, corresponding to ~3 × 10⁸ cells. Total RNA was extracted from the isolated Q cells using the Ribo-Pure™-Yeast Kit (Thermo Fisher Scientific), following the manufacturer's protocol. Each samples had two tech-nical repetition (1/2) that were then stored at –70°C. Library construction, sequencing, and the subsequent bioinformatic analysis were carried out by Novogene.