shotgun metagenomics of Dermacentor reticulatus
dissected tick ovaries and Malpighian tubules were pooled, homogenized, filtered, and centrifuged to further extract the total genomic DNA from the obtained pellet using the NucleoSpin Soil kit (Macherey-Nagel). Libraries were prepared with Nextera DNA Flex (Illumina) and then sequenced using the Illumina NovaSeq 600 technology by MGX DNA Services Facility (Montpellier, France)