GSM9407126: Dam_9h_Sample19_batch2; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions and 800ng of RNA was reverse transcribed with cDNA synthesis master mix (Legene Biosciences,CA, USA). Sequencing libraries were constructed using the TruSeq Stranded mRNA Library Preparation Kit (Illumina) and IDT for Illumina-TruSeq RNA UD Indexes. Libraries were sequenced on a NovaSeqX to a minimum depth of 35 million reads.