Whole Genome Analysis of Indian Isolates of the Fusarium graminearum
the PE illumina library was loaded onto Illumina for cluster generation and sequencing. Paired-End sequencing allows the template fragments to be sequenced in both the forward and reverse directions on Illumina. The kit reagents were used in binding of samples to complementary adapter oligoes on paired-end flow cell. The adapters were designed to allow selective cleavage of the forward strands after re-synthesis of the reverse strand during sequencing. The copied reverse strand was used to sequence from the opposite end of the fragment.