GSM9359716: Patient_5_Day_7; Homo sapiens; RNA-Seq
PBMC were isolated and cryopreserved. PBMC were thawed in batches, with all timepoints for a given patient thawed and analyzed at once. Five total batches: Patient 2, Patients 3 & 4, Patient 5, Patient 6, Patients 7 & 8. Cells were then stained with fluorescently-labeled antibodies specific for canonical cell lineage markers, fixable Live/Dead stain, and in some cases hashing antibodies. Live cells were isolated via FACS Aria before analysis by 10X. Library construction was performed according to manufacturer's protocol using 10X genomics single cell 3' v3 chemistry.