Responsiveness to 2FL and GOS by infant gut microbiota differs by history of individual 2FL consumption
DNA was extracted from 0.25g of each stool aliquot frozen without buffer using the QiaAmp FecalPro extraction kit (Qiagen) as per manufacturers instructions. DNA concentration was measured using Qbit Amplified library generation with Nextera XT adapters, and sequencing was performed to obtain 150bp DNA paired end reads to a depth of 10 million base pairs per sample using an Illumina NovaSeq 6000 apparatus (Illumina Corp.).