16S amplicon seq of cultivable SS AMD bacteria
Bacterial 16S amplicon sequencing library was prepared using DNA, extracted from the cultivated SS abandoned mine drainage sample with ZymoBIOMICS DNA Kit, as a template and 341F: 5-CCTAYGGGRBGCASCAG-3 and 806R: 5-GGACTACNNGGGTATCTAAT-3  barcoded primer pair, targeting V3-V4 hypervariable region of 16S rRNA genes. Sequencing was performed by Novogene sequencing provider on the Illumina platform to generate 250 bp paired-end raw reads, 30K tags per sample.