Characterization of arterial dendritic cells in giant cell arteritis and polymyalgia rheumatica
Characterization of arterial dendritic cells in giant cell arteritis and polymyalgia rheumatica
FFPE tissue sections from control (n=3), polymyalgia rheumatica (n=5) and giant cell arteritis (n=3) temporal arteries biospies Total RNA was extracted from FFPE tissue sections using the Maxwell RSC RNA FFPE kit (Promega) according to the manufacturer's protocol. Ribosomal RNA-depleted RNA was used for library preparation with the NEBNext Ultra II Directional RNA library prep kit for Illumina (New England Biolabs) according to the manufacturer's instructions. Libraries were paired-end sequenced (2*76 base pairs) on a NextSeq500 device (Illumina), with a read depth of 40 million