patient c13 Saliva mycobiome
Primers targeting the fungal internal transcribed spacer 1 (ITS1) region of the rRNA operon (forward: TAGAGGAAGTAAAAGTCGTAA; reverse: TTYRCTRCGTTCTTCATC; IUPAC degeneracies) were used (Toju et al., 2012), with Illumina adapter overhangs, for sequencing on an Illumina MiSeq platform. Sequencing was performed at the Foundation for the Promotion of Health and Biomedical Research (FISABIO, Valencia, Spain).