RNA-seq of ticks: Dersert tick-brone Virus
Raw paired-end sequence reads were first quality controlled using AfterQC (v2.3.3), and host-related reads were removed by mapping against the tick genomes (GCA_013339685.2 and PRJNA1131278) downloaded from the NCBI using Bowtie2 (v2.3.5.1).Clean reads were de novo assembled into contigs using Trinity (v2.13.2).The assembled contigs were compared to the NCBI non-redundant protein database using Diamond BLASTx (v2.0.13) and to the NCBI nucleotide sequence database using BLASTn (v2.12.0+) to identify virus-related contigs