GSM8645376: Gli1,replicate 5,scRNAseq; Mus musculus; RNA-Seq
Murine bone marrow from long bones and spine was crushed, flushed, RBC lysed, and MACS-depleted for CD11b, Gr1, Ter119, CD4, CD8, B220, CD45, and CD71, and subsequently combined with the digested bone chip fraction, after which the samples were FACS-sorted for lineage-, tdTom+ viable cells for sequencing All protocols to generate scRNA-seq data on the 10x Chromium platform, including sample prep, library prep, instrument and sequencing settings, are available here:https://support.10xgenomics.com/single-cell-gene-expression