RT112 Treat rep2
Erdafitinib and erlotinib were purchased from MedChemExpress (Monmouth Junction, NJ). Gemcitabine (Gem) and cisplatin (Cis) were obtained from SelleckChem (Houston, TX). Human bladder tumor cell lines RT112 was purchased from Creative Bioarray (Shirley, NY). EV was collected from the clinical pharmacy at the MD Anderson Cancer Center. All other cell lines were obtained from ATCC (Manassas, VA). Cells were maintained following vendors instruction and seeded in 96-well plates for designed experiments. Luminal-type cell lines (RT4, RT112, UM-UC-14) were treated with erdafitinib and basal-type cell lines (5637, HT1376, SCaBER) were treated with the erlotinib for 3 or 5 days. For chemo / EV treatment, only basal-type cell lines (5637, HT1376, SCaBER) were treated with Gem/Cis or EV for 48h.