RNAseq of pre-parasitic second stage juveniles of M. chitwoodi race 1 replicate 1
newly hatched M. chitwoodi race 1 J2s was collected and Total nematode RNA was extracted using PureLink RNA Mini Kit (ThermoFisher). High quality total RNA (1-2 g, 260/280 = 1.8-2.2, with RIN value of RNA of at least 8.5) was packed on dry ice and sent to Novogene Co. Ltd for library preparation and sequencing (30M reads per sample with PE150) using the Illumina platform.