GSM8717169: Nascent RNA Alkbh5 KO 10min rep1; Mus musculus; RNA-Seq
After EU incubation, cells were lysed with TRIzol (Invitrogen), and total RNA was extracted. An equal amount of RNA extracted from EU-labeled Drosophila cells was added to each sample as a spike-in for further normalization. Nascent RNA was captured using the Click-iT Nascent RNA Capture Kit (Invitrogen) according to the manufacturer's instructions. RNA libraries were constructed using the SMARTer Stranded Total RNA-seq Kit v2 (Takara) according to the manufacturer's protocol.