GSM8773499: KO, biol rep 1, Stag2-KO mouse urothelium, 1 week after TMX induction; Mus musculus; ATAC-seq
ATAC-Seq was conducted as described by Alonso-Curbelo et al 2021 using 50,000 viable GFP+ urothelial cells (per condition) isolated by FACS. ATAC-Seq libraries were prepared using the NEBNext 2 X PCR Master Mix (NEB) as described (Corces et al. 2017) and purified using 1.8 X AMPure XP beads (Beckman Coulter).