RNA-Seq of  soybean rhizosphere soil microbiome: control group
Collect soybean rhizosphere soil samples from laboratory potted plants (control group) under saline alkali stress. Extract total genomic DNA from soil samples and amplify bacterial 16S rRNA genes using primers. Construct an Amplicon library according to the standard Illumina library preparation protocol and perform paired end sequencing on the Illumina platform to analyze the composition of rhizosphere microbial communities.