GSM9588481: RNAseq_Khdrbs1-KO_rep2; Mus musculus; RNA-Seq
Total RNA was extracted from mES cells using the NucleoSpin RNA Mini Kit (Macherey-Nagel), following the manufacturer's protocol. RNA concentration and purity were assessed using a NanoDrop spectrophotometer, and RNA integrity was evaluated using the Agilent 2100 Bioanalyzer with RNA 6000 Nano chips (Agilent Technologies) to determine RNA Integrity Numbers. Only samples with RIN ≥9 were selected for downstream processing. To enable normalization and monitoring of technical variation, the ERCC RNA Spike-In Mix (Thermo Fisher Scientific) was added to each sample prior to library preparation. RNA sequencing libraries were prepared by Macrogen Inc. (Seoul, South Korea) using the TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat (Illumina). Libraries were sequenced on an Illumina platform with 150 bp paired-end read configuration.