RNA-Seq of Mus musculus: colon tissue under graded DSS-induced colitis
Total RNA was isolated from mouse colon tissues and assessed for quality prior to library construction. Poly(A)+ mRNA was enriched, fragmented, and reverse-transcribed into cDNA. RNA-seq libraries were prepared using the Illumina TruSeq RNA Sample Preparation Kit and sequenced on an Illumina NovaSeq 6000 platform with 150 bp paired-end reads.