GSM9360758: Mouse Tumor, B16F10, rep2, scRNAseq, mMo.DT4.2; Mus musculus; RNA-Seq
To differentiate intravascular and extravascular leukocytes, mice were injected intravenously with APC-Cy7-conjugated anti-CD45 antibody 5 minutes before harvest. Lung single cell suspensions were enriched using CD11b Miltenyi beads. Extravascular monocyte-macrophage populations, CD64+CD11b+ cells, were sorted using the FACS Aria Fusion (BD Biosciences). Approximately 30,000 cells per sample were loaded on the Chromium Next GEM Single Cell 3′ Platform (10x Genomics) and sequenced on an Illumina NextSeq 500/550 with an average depth of approximately 50,000 reads per cell.