Rainbow trout adipose tissue depots (VAT, SAT, IMAT) - mRNAs
Rainbow trout adipose tissue depots (VAT, SAT, IMAT) - mRNAs
Adipose tissue was dissected from fish (visceral, subcutaneous, and intramuscular depots). Mature adipocytes were isolated by mechanical and enzymatic digestion using collagenase type II. Total RNA was extracted using TRIzol reagent (Thermo Fisher Scientific, Waltham, MA, USA) followed by isopropanol, chloroform, and ethanol extraction according to the manufacturer's instructions. RNA libraries were prepared by Azenta/GENEWIZ (Leipzig, Germany) using Illumina RNA library preparation with poly(A) selection according to the company's standard protocol.