GSM9590069: GC_3; Mus musculus; RNA-Seq
Total RNA was extracted from powder samples using ISOGEN II reagent (Nippon Gene) according to the manufacturer's instruction. About 500 ng of total RNA were ribosomal RNA-depleted using NEBNext rRNA Depletion Kit (E6310, New England Biolabs). RNA libraries were prepared for sequencing using standard Illumina protocols.