GSM8646270: NMP, replicate 2, snRNA; Homo sapiens; RNA-Seq
Nuclei isolation followed the 10X Genomics Demonstrated Protocol GC00365 Nuclei Isolation for Single Cell Multiome ATAC + Gene Expression Sequencing. The Dnase treatment step was included and the lysis buffer was adjusted to 0.8x strength. Library construction followed 10X Genomcis Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Protocol (CG000338 Rev E). Final libraries were quality-controlled on an Aligent 5200 Fragment Analyzer.