16S_DNA_Gut_HiLo
Platform: MiSeq; Paired  end: Yes; Read length (bp) : 300; Primer pair: V1-V2: 28F-YM:28F-388R Borrellia:28F-388R Chloroflex: 28F-388RBifdo at a ratio of 4:1:1:1 ; DNA polymerase : Kapa ready mix hotstart hi-fi polymerase ;PCR cycle No. : 30; Adapters:Nextera XT;Sequencing depth  (avg reads per sample): 146041; Library prep and sequencing protocol: Illuminas 16S Metagenomic Sequencing Library Preparation Protocol and a MiSeq 2 x 300-cycle v3 kit