GSM8615469: pl06; Homo sapiens; RNA-Seq
PBMCs were unfrozen, rested overnight, washed, and prepared for single-cell capture using the 10X workflow. Immediately prior to the capture, cells from different individuals were combined into pools. Single-cell GEMs were generated using a Chromium Controller instrument (10x Genomics). Sequencing libraries were prepared using Chromium Single Cell 3' Reagent Kits (v3.1 Dual Index chemistry, 10x Genomics) according to the manufacturer's instructions.