DNA sequencing of Eschirichia coli KD504: self targeting of type I-E CRISPR-Cas, 0h after induction
Sequencing libraries were constructed from 1 g of total genomic DNA usingMGI Easy PCR-Free Library Prep Set in accordance with User Manual v1.1 (MGITech Co., Shenzen, China), size-selected and cleaned up with the provided DNAClean Beads. Quality control was performed with Agilent High SensitivityD1000 ScreenTape Assay on 4200 TapeStation System (Agilent TechnologiesInc., Santa Clara, CA, USA) and QuDye ssDNA kit (Lumiprobe, Moscow, Russia).Barcoded libraries were pooled and sequenced using DNBSEQ-G400 in2x150bp PE mode.