Illumina MiSeq paired Sequencing of pair_METAGENOMIC_GM002 (KAS24119365) (KAE24094570)
To determine whether alterations in microbial activity were indicative of changes within the bacterial community, we extracted genomic DNA from the samples and prepared sequencing libraries targeting the V3 and V4 region of the 16S rRNA gene using specific primers (341F: CCTACGGGNGGCWGCAG and 805R: GACTACHVGGGTATCTAATCC). Sequencing was performed on the Illumina MiSeq platform.