GSM8666425: Treg from Crlf2fl/fl Foxp3YFP-Cre rep3; Mus musculus; RNA-Seq
Cells were harvested and the RNA-seq libraries were prepared with 100 -500 ng of total RNA according to the manufacturer's instructions. The fragment size of RNA-seq libraries was verified using the Agilent 2100 Bioanalyzer (Agilent) and the concentration were determined using Qubit (LifeTech). The libraries were loaded onto the Illumina's NovaSeq 6000 to generate 50 bp single end sequencing reads.