Illumina whole genome sequencing of Pasteurella multocida
The isolate was streaked out on blood agar. A single colony was inoculated into Nutrient Broth and grown at 37C. Genomic DNA was extracted by MicrobesNG according to their protocols. Genomic DNA libraries were prepared using the Nextera XT Library Prep Kit (Illumina, San Diego, USA) following the manufacturers protocol with the following modifications: input DNA was increased 2-fold, and PCR elongation time was increased to 45 seconds. Libraries were sequenced on an lllumina NovaSeq 6000 (Illumina, San Diego, USA) using a 250 bp paired end protocol. Reads were adapter trimmed using Trimmomatic version 0.30 with a sliding window quality cutoff of Q15.