GSM8807175: Col_H3K27me1_replicate1; Arabidopsis thaliana; ChIP-Seq
Nuclei were extracted, and mononucleosomes were generated using micrococcal nuclease (NEB, M0247S) digestion.Immunoprecipitation was conducted with anti-H3K27me1 ( (Abclonal, A22170). The input DNA and immunoprecipitated DNA were subjected to library preparation with VAHTS universal DNA library prep kit for illumina (Vazyme, ND607-02) according to the manufacturer's instruction.