16S of Human gut microbiota
16S rRNA sequencing library were constructed following the 16S rDNA gene Metagenomic Sequencing Library Preparation Illumina protocol, targeting the V3 and V4 hypervariable regions. After 16S rDNA gene amplification, the multiplexing step was performed using Nextera XT Index Kit. The libraries were sequenced using a 2x300pb paired end run on a MiSeq Sequencer.