GSM9409576: Nasal cells, nasal brushing; Homo sapiens; RNA-Seq
To collect nasal brushing(NB) samples, flocked swabs (Pap brush; SGS, Daejeon, Korea) were inserted into the nasal cavity of the donors and rotated 10–20 times. The swabs were then placed in tubes containing Dulbecco's Modified Eagle's Medium/F-12 (1:1) (1X) media (Gibco, Grand Island, NY, USA). The tubes were vigorously vortexed to dislodge the cells and pelleted via centrifugation. Nasal tissue (NT) was mechanically dissociated, and single-cell suspensions were prepared using a Tumor Dissociation Kit (Miltenyi Biotec, Auburn, CA, USA) in combination with a gentleMACS dissociator (Miltenyi Biotec) and 70-µm cell strainers (SPL Life Sciences, Gyeonggido, Korea). The scRNA-seq libraries were prepared using the 10x Genomics Chromium Next GEM Single Cell 3` v3.1 chemistry. Libraries were constructed and sequenced at a depth of approximately 20,000 reads per cell using the NovaSeq X Plus platform (Illumina, San Diego, CA, USA).