GSM9284624: THP1_Ctrl_2; Homo sapiens; RNA-Seq
Five million AML cells were plated and treated with DMSO or 50 nM IACS-010759 for 8 or 24 h for three independent replicates. Total RNA was isolated using a RNeasy kit (Qiagen) with the on-column DNA digest following the manufacturer's protocol. RNA sequencing libraries for each sample were prepared with 1 ug total RNA by using the TruSeq RNA Sample Prep v2 Kit (Illumina) per the manufacturer's instructions, and sequencing was completed on the Illumina NovaSeq 6000.