RNA-Seq of homo sapiens: breast tissue
Our approach, mFISHseq, integrates multiplexed RNA fluorescent in situ hybridization with RNA-sequencing, guided by laser capture microdissection. The Macherey Nagel NucleoSpin totalRNA FFPE XS kit was used for RNA isolation. We used the Takara SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian kit to prepare total RNA-SEQ libraries following the manufacturers instructions. To control for batch library preparation effects, we included a single natural positive control sample in each library preparation batch and a synthetic spike-in control in each sample