Resequencing Library of PmPBDH in Triticum aestivum (PBDH1607)
Fresh leaf tissues of PBDH1607 were sampled at four-leaf stage for DNA extraction and purity assessment following the established procedure. Genomic DNA was extracted and rigorously quality-controlled, with only intact molecules (15-20 kb) proceeding to library preparation. The size-selected DNA was used to construct PacBio HiFi sequencing libraries, which were then sequenced on the Pacific Biosciences Revio platform (HAORUI GENOMICS, Xi'an, China) following standardized HiFi protocols. Three PacBio SMRT cells were employed to generate >90 Gb high-fidelity sequence data per cell, ensuring sufficient coverage for downstream analyses.