GSM9450223: LAD2 CUT&Tag JUND SCF- biol rep 2; Homo sapiens; OTHER
CUT&Tag was performed using concanavalin A (ConA) magnetic beads to immobilize cells, followed by permeabilization with digitonin. Cells were incubated with primary anti-JUND antibody (Active Motif 61403; RRID:AB_2793621) and secondary antibody, then with pA/pG-Tn5 transposase to tagment chromatin near antibody-bound sites. DNA fragments were released after tagmentation for library amplification. Libraries were prepared using Hyperactive™ In-Situ ChIP Library Prep Kit for Illumina (Vazyme; TD903–TD904) following the manufacturer's instructions. Tagmented fragments were PCR-amplified with P5/P7 indexed primers, purified, and assessed on an Agilent 2100 Bioanalyzer. Libraries were sequenced as paired-end reads on an Illumina instrument.