Bacillus subtilis NRS7979 Genomic Sequencing
Genomic DNA was extracted using the QIAprep Spin Miniprep Kit (cat. Nos. 27104 and 27106) and following the 'Quick-Start Protocol'. DNA concentration was measured using a Qubit 4 Fluorometer or a NanoDrop One/OneC Microvolume UV-Vis Spectrophotometer. Illumina sequencing libraries were prepared by SeqCentre using the tagmentation-based and PCR-based Illumina DNA Prep kit and custom IDT 10bp unique dual indices (UDI) with a target insert size of 280 bp. No additional DNA fragmentation or size selection steps were performed. Illumina sequencing was performed on an Illumina NovaSeq X Plus sequencer in one or more multiplexed shared-flow-cell runs, producing 2x151bp paired-end reads. Demultiplexing, quality control and adapter trimming was performed with bcl-convert1 (v4.2.4). 