RNAseq 20His_rep1
Total RNA was extracted from third instar larvae using TRIzol reagent.  mRNA was enriched using poly(A) selection and used to prepare strand-specific libraries. Library quality was verified on the Bioanalyzer, quantified by qPCR, and sequenced on an Illumina NovaSeq 6000 platform to generate 150 bp paired-end reads.