RNA-Seq of Lepidodactylus lugubris
Bulk RNA seq data was collected from the following L. lugubris tissues and organs: heart, skeletal muscle, brain, liver, lung, gonad, gallbladder, spleen, egg, and bone. Total RNA was isolated using the Qiagen RNeasy Kit following manufacturer protocols. Samples were sent to Novogene for library preparation and sequencing. mRNA libraries were prepared using poly-A enrichment, and sequencing was performed on the Illumina NovaSeq X Plus platform with paired-end 150 bp reads. This RNA seq data was used to perform genome annotation using the NCBI Eukaryotic Genome Annotation Pipeline [28]. This pipeline uses both RNA-seq data and homology-based evidence for gene prediction.