Short-reads female M. macrocephalus
Tissue samples for genome sequencing were obtained from an adult ZW female Megaleporinus macrocephalus from the broodstock of the Aquaculture Center of So Paulo State University. To confirm the genotype of the individual, we performed cytogenetic analysis using the lymphocyte culture technique described by Bertollo, Moreira-Filho & Galetti (1986) with some adjustments, and C-banding according to Sumner (1972). To improve the accuracy of the long reads, a short read library was produced using the MGIEasy PCR-Free Library Prep Set.