GSM9629207: ChIPseq human melanoma Sbcl2_n2 (PolII ChIP); Homo sapiens; ChIP-Seq
ChIP was performed with the melanoma cell lines and the normal human epidermal melanocytes (NHEM) that had not exceeded six passages. Chromatin  was crosslinked in 1/10th volume of fixation buffer (50 mM HEPES/KOH, pH 7.9, 11% formaldehyde). After quenching with glycine and lysis the chromatin was sheared to an average DNA fragment size of ~400–600 bp using a Covaris ME220. ChIP-seq libraries were generated using the TruSeq ChIP Library Preparation Kit (IP-202-1012, Illumina Inc., San Diego, CA, USA) following the recommended workflow. Library quality and fragment size distribution were assessed using a 4200 Tape Station (Agilent Technologies), and concentrations were determined by Qubit fluorometric quantification. Prepared libraries were sequenced on an Illumina NextSeq2000 using a P4 Flow-cell with 72 bp single-end reads.