Transcriptome of Acinetobacter pittii CEP14 grown on pyruvate only, replicate 2
Samples for transcriptomic analyses were taken during the period of maximal cDCE depletion, when the expression of catabolic genes involved in cDCE degradation is theoretically at its highest (CEP14: after 9 hours, JAB1: after 16 hours). Bacterial pellets were resuspended in a solution containing RNA Protect Reagent (Qiagen, Hilden, Germany) and treated with lysozyme (1 mg/ml). Cells were centrifuged and lysed in RLT buffer (Qiagen) containing beta-mercaptoethanol. RNA extractions were performed on RNeasy Mini spin columns following the manufacturers protocol (Qiagen), including on-column DNase I digestion.