GSM8773177: mES_BADC_ATAC_rep2; Mus musculus; ATAC-seq
Cells were dissociated with Accutase for 5min at 37°C, washed and collected in cold resuspension buffer (RSB). 50k cells were resuspended in lysis buffer and incubated for 3min on ice. then immediately washed with RSB containing 0.01% Tween-20 and centrifuged. The pellet was gently resuspended in 50μL Tagmentation Mix (Nextera) and incubated at 37°C in a thermomixer at 1000rpm for 30min. DNA was extracted using the MinElute PCR Purification Kit (Qiagen), then fragments were amplified with Nextera Index Primers (Nextera) using the KAPA HiFi HotStart ReadyMix (Roche) for 12 cycles. After PCR the reactions were cleaned first with the MinElute PCR Purification Kit and then with AMPure XP beads (Beckman Coulter). Library quality was assessed with a fragment analyzer before sequencing.