Metagenome of PHBH degrading anaerobic digester
PCR amplification of targeted regions was performed by using specific primers connecting with barcodes. The PCR products of proper size were selected through agarose gel electrophoresis. The same amount of PCR products from each sample was pooled, end-repaired, A-tailed, and further ligated with illumina adapters. Libraries were sequenced on a paired-end Illumina platform.