16S rRNA-Seq of Enterobacter sichuanensis:Laboratory anaerobic reactor
27F and 1492R. The sequences are AGRGTTYGATYMTGGCTCAG and RGYTACCTTGTTACGACTT respectively. PCR uses TransStart Fastpfu DNA Polymerase, with a 50 l reaction system. The conditions are as follows: a. 1 (5 minutes at 95C) b. 27 (30 seconds at 95C; 30 seconds at 55C; 45 seconds at 72C) c. 10 minutes at 72C, and continue at 10C until stopped by the user.