DNA-seq of Microbacterium chocolatum_Mc_TGS
For PacBio sequencing, SMRTbell libraries were constructed using the Express Template Prep Kit 2.0 (Pacific Biosciences, CA, USA) according to the manufacturers protocol. Raw Illumina reads were qualityfiltered with Trimmomatic (SLIDINGWINDOW: 4:15, MINLEN:75) (Bolger et al., 2014), and raw PacBio reads were converted to fasta format using Samtools. Hybrid de novo genome assembly was performed using Unicycler with default parameters, integrating short and long reads to improve assembly continuity and accuracy (Wick et al., 2017).