GSM8740325: Anterior prostate FVB mouse 1; Mus musculus; RNA-Seq
RNA was purified from the intervening sections using the AllPrep DNA/RNA Kit (Qiagen) per the manufacturer's recommended protocol. Tissue was homogenized in Buffer RLT (from AllPrep kit) using gentleMACS M tubes (Miltenyi Biotec) and dissociated using program RNA_02, and subsequently passaged through a Qiashredder column (Qiagen). Ribosomal RNA depletion was performed using the Ribo-Zero Mouse rRNA Removal kit using total RNA (500 ng) as input (Illumina). RNA-seq libraries were prepared using TruSeq Stranded Total RNA Library Kit (Illumina), and paired-end sequenced in 2 x 150-bp format on the NovaSeq6000 S4 platform (Illumina).