GSM8773164: human arteriole snRNA-seq; Homo sapiens; RNA-Seq
Human arterioles were broken up using a beads method, and nuclei were extracted using the 'Frankenstein' protocol.  Briefly, the arterioles were broken up in 0.5 ml Nuclei EZ Lysis buffer with Precellys Evolution Touch and tissue homogenizing ceramic beads at a setting of 6,500rpm for 20 seconds.  Another 3.5 ml Nuclei EZ Lysis buffer was added to tissue homogenized buffer and incubated for 5 minutes on ice before being filtered through a 70 µm strainer mesh.  The extracted nuclei were washed once with Nuclei Wash and Suspension Buffer. Single-nucleus RNA-seq libraries were prepared using Chromium Single Cell 3' Reagent Kits V3 (10x Genomics, Pleasanton, CA), following the manufacturer's user guide.