GSM8880049: Losartan treated , 7 Days , rep 3; Mus musculus; RNA-Seq
Total RNA was isolated using TRIzol reagent (Invitrogen). RNA quality was assessed using an Agilent 2100 bioanalyzer and an RNA 6000 Nano Chip (Agilent Technologies, Amstelveen, The Netherlands) and RNA quantification was performed using an ND-2000 Spectrophotometer (Thermo Inc., DE, USA). For control and test RNAs, library construction was performed using the QuantSeq 3' mRNA-Seq Library Prep Kit (Lexogen, Inc., Austria) according to the manufacturer's instructions.