GSM9449271: scRNA-seq of KPC tumor from Porcn WT mice; Mus musculus; RNA-Seq
KPC tumors were excised from WT (n=5) and KO (n=5) mice, with samples from each genotype pooled separately. Tumors were minced and enzymatically digested with Collagenase V (1.5 mg/mL) and DNase I (0.1 mg/mL) at 37°C for 30 minutes. The single-cell suspension was filtered, red blood cells were lysed using ACK buffer, and viable single cells were enriched for downstream single-cell RNA-sequencing. Single-cell RNA libraries were prepared using the Chromium Next GEM Single Cell 3' RNA library v3.1 (10x Genomics) following the manufacturer's instructions. Libraries were sequenced on an Illumina NovaSeq 6000 platform.