GSM9359494: dPC12EVs-2; Rattus norvegicus; RNA-Seq
Total RNA was extracted using QIAzol and the miRNeasy Mini Kit (Qiagen, Hilden, Germany) according to manufacturer's protocols. RNA quantity and quality were evaluated with a Nanodrop ND-1000 spectrophotometer (Thermo Fisher Scientific) and an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) RNA libraries were prepared using NEBNext Ultra II Directional RNA Library prep for Illumina, NEBNext rRNA Depletion Kit and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs), and yields were evaluated using Agilent 2100 BioAnalyzer.