GSM9407030: Wild-type HCT116, technical replicate2; Homo sapiens; RNA-Seq
Total nuclear RNA was isolated from biological and technical replicates using the NucleoSpin RNA Plus Mini kit with on‑column DNase treatment (Macherey‑Nagel, 740984). Polyadenylated RNA was enriched using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB #E7490). Libraries were prepared with the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB #E7765). Almost 2ug of mRNA was used for library preparation. No size selection was performed during library preparation.