GSM9285031: C2C12 cells, iMyoD1, no differentiation, untreated, RNA-seq biol rep 1; Mus musculus; RNA-Seq
Total RNA was extracted using TriZol following manufacturer protocols polyA-mRNA enrichment was performed on extracted RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490S). Extracted RNA was reverse transcribed following NEBNext Ultra II RNA Library Prep kit, following manufacturer protocol. Libraries were prepared following NEBNext Ultra II RNA Library Prep kit protocol