Disruption of macrophage cell volume drives inflammatory responses and type I interferon signalling
Disruption of macrophage cell volume drives inflammatory responses and type I interferon signalling
Primary BMDMs from CX3CR1-Cre LRRC8A flox and wild-type littermates (CX3CR1-cre negative, LRRC8A flox) were incubated in DMEM + 50% PBS or 50% H2O for 4 hours.   BMDMs were lysed in RNA lysis buffer (Purelink RNA miniprep kit). RNA was isolated using a Purelink RNA miniprep kit following the manufacturer's instructions.  RNA samples were assessed for quality and integrity using a 2200 TapeStation (Agilent Technologies) and then libraries were generated using the TruSeq® Stranded mRNA assay (Illumina, Inc.) according to the manufacturer's protocol. 