GSM8666089: Ctrl, skin fibroblasts (Input); Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. Then the steps were followed using Simple ChiP Plus Sonication Chromatin IP Kit (56383, Cell Signaling Technology) according to manufacturer's instructions. ChIP-seq of KDM6B in the fibroblasts depleted of KDM6A. Add "A" to the DNA sample after immunoprecipitation according to the kit for end repair; The product was purified by magnetic beads; The linker ligation reaction was configured, followed by purification and fragment sorting with magnetic beads; The PCR reaction system was configured and the reaction program was set to amplify the ligated products; The amplified PCR product was purified by magnetic beads to recover the target fragment, and the purified library was dissolved in elution buffer.