Bacillus subtilis NRS7209 Genomic Sequencing
Genomic DNA was extracted using the QIAprep Spin Miniprep Kit (cat. Nos. 27104 and 27106) and following the 'Quick-Start Protocol'. DNA concentration was measured using a Qubit 4 Fluorometer or a NanoDrop One/OneC Microvolume UV-Vis Spectrophotometer. Genomic DNA libraries were prepared using the Nextera XT Library Prep Kit (Illumina, San Diego, USA) following the manufacturerï¿½s protocol with the following modifications: input DNA was increased 2-fold, and PCR elongation time was increased to 45 s. DNA quantification and library preparation were carried out on a Hamilton Microlab STAR automated liquid handling system (Hamilton Bonaduz AG, Switzerland). Pooled libraries were quantified using the Kapa Biosystems Library Quantification Kit for Illumina. Libraries were sequenced using Illumina sequencers (HiSeq/NovaSeq) using a 250 bp paired end protocol. 