HiFi Sequencing
The HiFi sequencing experiment aims to generate highly accurate long-read sequences of the Brainea insignis genome, an endangered fern species, to improve genome assembly quality and provide a comprehensive view of its genomic structure. DNA was extracted from leaf tissues of multiple individuals, and libraries were prepared using the PacBio SMRTbell library preparation kit. Sequencing was performed on a PacBio Sequel II system, utilizing the HiFi sequencing mode, which generates long, high-fidelity reads with high accuracy. The objective of this experiment is to capture complex genomic regions, including structural variations, repetitive sequences, and haplotype phasing, which are challenging to resolve with short-read sequencing technologies. The generated data will be used for de novo genome assembly, refining the genome annotation, and investigating genomic features critical for the conservation and evolution of the species.