GSM9192814: crfvmate51_2_Rep3; Fragaria vesca; RNA-Seq
Total RNA was extracted from fruits of 26 DPA WT fruits and FvmMATE51 mutants (Fvmate51-1 and Fvmate51-2) at 26-day postanthesis using HiPure HP Plant RNA Mini Kit (Magen, China) Poly(A)+ mRNA was enriched using oligo(dT) beads, reverse transcribed with random hexamers to generate double-stranded cDNA. After end repair, A-tailing, and adaptor ligation, fragments were size-selected, amplified, and purified. Library quality was verified by Qubit, qPCR, and Bioanalyzer analysis. Eligible libraries were then pooled equimolarly and sequenced on an Illumina platform.