GSM8773645: WRC27, leaf, moderate drought, Day 5, rep 1; Oryza sativa; RNA-Seq
The fully expanded latest leaves and second latest leaves were collected and frozen in liquid nitrogen. Samples were ground to a fine powder with a multi-beads shocker and used for RNA extraction. Total RNA was extracted using guanidine isothiocyanate based buffer and magnetic beads as described previously (Yoshino et al., 2020). RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit (E7760L) according to the manufacture's protocol.