illumina and PacBIO Sequencing of Saccharomyces cerevisiae D452_2 (KAS24111159) (KAE24087905)
The PacBio sequencing library was prepared by fragmenting genomic DNA (gDNA) using a genomic DNA fragmentation kit, followed by purification and size analysis. Subsequently, SMRT adapters were ligated for library preparation. The prepared library was purified and then used for sequencing. The Illumina sequencing library was prepared using the TruSeq Nano DNA HT library preparation kit and was subsequently used for sequencing.