DNA sequencing of human betaherpesvirus 5: TB40E/5_UL138stop bacterial artificial chromosome
Bacterial artificial chromosomes containing the human betaherpesvirus 5 genome were isolated from Escherichia coli using the NucleoBond Xtra BAC kit for large construct plasmid DNA (Macherey-Nagel 740436.25). Plasmid DNA was resuspended at 200 ng/uL in 10mM Tris, pH 8.5. Whole genome sequencing was performed by Plasmidsaurus using Oxford Nanopore Technology with custom analysis and annotation.