Oxford Nanopore technology sequecing of ASF virus isotate 209 P50
The PCR-free library for Oxford Nanopore sequencing was prepared from 250 ng of high molecular weight genomic DNA using a 1D Native Barcoding Kit 24 V14 (SQK-NBD114.24; Oxford Nanopore Technologies, Oxford, United Kingdom). The library was loaded onto a R10.4.1 flow cell and ran for 12 h on a MinION Mk1C sequencer.