GSM9267641: MOC2_2; Mus musculus; RNA-Seq
Total RNA was extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. RNA quality and integrity were assessed with a TapeStation D5000 (Agilent Technologies), and samples with RIN values greater than 7 were used for library preparation. Poly(A) mRNA was enriched from total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturer's instructions to generate stranded paired-end libraries.