RNA-Seq of Cucumis sativus :  H70D-3
Total RNA was extracted using the guanidine thiocyanate method, and the quality and quantity of the RNA were assessed before proceeding to library construction and sequencing. Ribosomal RNAs were removed, and RNA libraries were constructed using the VAHTS Stranded mRNA-seq V2 Library Prep Kit for Illumina (NR601-01, Vazyme, China). RNA sequencing were performed on Illumina HiSeq 2000 system at the Beijing Novogene Institute.