Illumina of Pseudomonas
Bacterial isolate was sequenced using the Nextera XT DNA Library Preparation Kit. Genomic DNA was enzymatically fragmented and tagged with an adapter and the tagmented DNA was subjected to a limited-cycle PCR amplification of 12 cycles, incorporating unique dual-index barcodes for sample identification. The amplified library was purified using AMPure XP beads to remove short fragments and residual reaction components. Whole Genome Sequencing was performed on a NextSeq 2000 System using a NextSeq 1000/2000 P1 Reagent Kit (600 Cycles) for the production of 2 x 300 bp paired end reads