GSM8644553: Adult blood donor 8 0HR; Homo sapiens; RNA-Seq
Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. Sequencer Used: Novaseq 6000, S4 Flowcell, Paired-End 150 cycles After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or DTTP for non-directional library. For the non-directional library, it was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. For the directional library, it was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries were pooled and sequenced on Illumina Novaseq 6000, S4 Flowcell, Paired-End 150 cycles. Samples that passed QC were advanced to RNA sequencing and included in data analysis. Six samples for each age and time point were submitted, but only those that passed RNA quality control were advanced to sequencing and analysis.