GSM9511084: T cells from Donor 2 treated with a poly-functional editor targeting AAVS1 and epi-silencing B2M; Homo sapiens; OTHER
gDNA extraction by Maxwell RSC Cultured cells DNA Kit (Promega), following provider indications. Targeted genomic regions were amplified by PCR using locus-specific primers. A second round of PCR was performed to add full-length Illumina adapters and sample-specific barcodes. Libraries were beads-purified, quantified, pooled at equimolar concentrations, and sequenced using an Illumina MiSeq platform in a paired-end mode generating 2x250 bp reads.