Identification of tens of thousands of novel gene loci in the human and mouse genomes through targeted full-length long-read RNA sequencing
Identification of tens of thousands of novel gene loci in the human and mouse genomes through targeted full-length long-read RNA sequencing
Commercial samples obtained from Ambion (Thermo Fisher Scientific) or Clontech-Takara Bio: Both Ambion and Clontech-Takara Bio are brands specializing in life science research products, with a focus on RNA technologies. Ambion is a brand owned by Thermo Fisher Scientific, a global provider of scientific research products and services, while Clontech is part of Takara Bio, known for molecular biology tools. Therefore, \"commercial samples obtained from Ambion (Thermo Fisher Scientific) or Clontech-Takara Bio\" refers to RNA samples purchased from these brands for research purposes. Total RNA was extracted from the collected samples using protocols performed by commercial providers Ambion (Thermo Fisher Scientific) or Clontech-Takara Bio. Although the exact kit or extraction protocol used was not specified, the RNA extraction followed the manufacturers' standard procedures, ensuring high-quality and reliable RNA yield for downstream applications. SMARTerStranded: The SMARTer Stranded RNA-Seq protocol is a next-generation sequencing method designed for generating high-quality, strand-specific RNA libraries for Illumina short-reads. It preserves strand information by incorporating adapters to maintain the orientation of RNA transcripts.