GSM8743011: CD248_pos_4; Mus musculus; RNA-Seq
Total RNA from FACs sorted CD248+ and CD248- fibroblasts was isolated using the RNeasy Mini Kit (Qiagen), and RNA purity and quantification were assessed using the Qubit 2.0 fluorometer (Life Technologies). RNA integrity was evaluated using the 2100 Bioanalyzer (Agilent Technologies). Libraries were prepared with the TruSeq Stranded Total RNA Sample Preparation kit (Illumina)