Single nuclei RNA-seq of spinal cord from Eif2b5[R191H] mice
Single nuclei RNA-seq of spinal cord from Eif2b5[R191H] mice
Mice were euthanized with CO2, and the cervical and thoracic region of each spinal cord was freshly collected and snap frozen in liquid nitrogen and stored in liquid nitrogen Nuclei were isolated using the Chromium Nuclei Isolation Kit with RNAse Inhibitor (10x Genomics #1000494)  libraries were prepared according to Chromium Next GEM Single Cell 33â€™ Kit v3.1 instructions (10x Genomics #1000268) to capture 10,000 nuclei per sample. 