GSM8744982: Patient 103 PBLs sample collected at baseline; Homo sapiens; RNA-Seq
Total RNA was extracted and purified from frozen PBL cell pellets using Invitrogen PureLink RNA Mini kit (ThermoFisher Scientific, #12183018A). RNA sample quantity and quality were measured by Qubit RNA HS assay kit and Agilent Bioanalyzer RNA Nano chip to confirm RNA integrity, respectively. Three hundred nanogram of total RNA was used to construct RNA-seq library using NEBNext Ultra II Directional RNA Library Prep kit for Illumina (NEB #E7765L) according to manufacturer's protocol.