16S amplicon sequencing of cDNA ANS+ThickSlick Day 18 C1
RNA isolated from samples using the AllPrep Bacterial DNA/RNA/Protein Kit (Qiagen). cDNA was synthesized from the purified RNA extracts using the Invitrogen SuperScript IV First-Strand synthesis kit using random hexamer primers (Thermo Fisher Scientific).  Synthesized cDNA sequenced using Illumina MiSeq (San Diego, CA, USA) targeting the V4 region of the 16S rRNA gene (primers 515F & 806R) at the Cincinnati Children's Hospital Medical Center Genomics Sequencing Facility.