GSM8807117: eHAP iCas9, Control Day14, Replicate 1; Homo sapiens; OTHER
Genomic DNA was extracted from 500M cells per condition using a Quiagen Blood Maxi kit, per manufactururs protocol. Extracted genomic DNA was used as template for a primary PCR amplification of the guide-harboring locus. This  amplicon was purified by gel extraction, concentrated, and used for a secondary PCR with illumina indexes unique to each sample.