Enhancement of colorectal cancer therapy through interruption of the HSF1-HSP90 axis by p53 activation or cell cycle inhibition
Enhancement of colorectal cancer therapy through interruption of the HSF1-HSP90 axis by p53 activation or cell cycle inhibition
RNA samples were generated as pools. In detail, two biological replicates, each consisting of two pooled biological replicates per treatment group (total n = 4 per group; DMSO, Ganet, RG-7388, combi) were analyzed. The cells were cultured in RPMI 1640 supplemented with 10 % fetal bovine serum, glutamine and penicillin/streptomycin in a humidified atmosphere at 37°C with 5 % CO2. HCT116 cells were treated for 24 hrs with DMSO, 50 nM Ganetespib, 1 µM RG-7388 or in combination. The TRIZOL reagent from Invitrogen has been used for RNA extraction. Library construction performed by NovogeneNEB Next® Ultra™ RNA Library Prep Kit Library quality control: Agilent bioanalyzer 2100, DNA high-sensitivity chip Concentration checked by qPCR.