GSM9627753: mESC_EV_Myxothiazol_rep2_ATAC; Mus musculus; ATAC-seq
Approximately 50,000 cells were pelleted by centrifugation, washed with PBS, and nuclei were isolated using a cold lysis buffer. We performed the transposition reaction using the Illumina Tagment DNA Enzyme and Buffer Large Kit (Illumina, catalog no. 20034198) and purification steps with the MinElute PCR Purification Kit (Qiagen, catalog no. 28004). We then performed the PCR amplification step using the KAPA HiFi HotStart Ready Mix master mix (KAPA, catalog no. KR0370). To remove small fragments, we performed size selection using KAPA pure beads: 40 µL of KAPA beads were added to 50 µL of the PCR solution. We ran 2 µL of the library on TapeStation to verify the nucleosome footprints and confirm a successful assay.