16SrRNA for mARC2s Mus musculus
Genomic DNA was isolated from fecal samples using a QIAamp Fast DNA Stool Mini Kit . The quality and quantity of the extracted DNA was assessed by measuring the optical density in a NanoDrop 2000/2000c spectrophotometer and fluorometrically using a Qubit dsDNA HS Assay Kit. Preparation of the variable V3 and V4 region libraries from the 16S bacterial 16S rRNA gene was carried out in accordance with the 16S Metagenomic Sequencing Library Preparation protocol on an Illumina platform. Sequences were obtained by an Illumina MiSeq system, with paired-end reads of 2 300 bp.