GSM9191911: Female offspring of paternal ELS-HFD, brain, chow-diet, rep1; Rattus norvegicus; RNA-Seq
RNA and DNA were extracted using the Norgene RNA/DNA Purification micro kit ~400ng of purified RNA was used for library preparation using IDT xGen™ Broad-Range RNA Library Preparation Kit – the RNA sample is first enriched for mRNA using Poly-A beads. The RNA is fragmented using RNA fragmentation. The RNA is converted to cDNA using random primers. The r2 adapter is ligated to a single stranded cDNA molecule. That fragment is then extended using the extension primer. Then an R1 Stubby adapter is added followed by an indexing PCR Step for the final library.