GSM9267369: 95D cells, glucose deficiency; Homo sapiens; RIP-Seq
Total RNA was isolated from the cultured cells using TRIzol reagent (Invitrogen) following the manufacturer's instructions. 1-2 µg of total RNA was reverse transcribed using the HiScript III RT SuperMix with gDNA wiper (Vazyme). Libraries were constructed from the extracted total RNA using standard protocols, including rRNA depletion, fragmentation, cDNA synthesis, and adapter ligation. The Qualified libraries were pooled and sequenced on Illumina platforms with PE150 strategy in Novogene Bioinformatics Technology Co., Ltd (Beijing, China), according to effective library concentration and data amount required.