GSM8493843: vegetative wt cells, biol rep 1; Schizosaccharomyces pombe; RNA-Seq
Total RNAs were extracted using the hot acid phenol method. Total RNAs were subjected to ribosomal RNA depletion using the TruSeq Stranded Total RNA library prep Gold kit (Illumina, #20020598). 350 ng RNA was polyadenylated in vitro and librairies were prepared using the Direct RNA Sequencing kit (Oxford Nanopore Technologies, SQK-RNA004). direct RNA-seq (Oxford Nanopore Technologies)