DNA-seq of Drosophila mdg4 induced X-linked female sterile alleles
Genomic DNA was isolated from 30 females with the Qiagen blood and tissue kit. DNA was sonicated for 10 cycles using a Diagenode Bioruptor Plus sonication device. 5 ug of input DNA was used in the NEBNext Ultra II DNA Library Prep Kit for Illumina. The libraries were sequenced using the NovaSeq Xp work flow cell on the NovaSeq 6000 System.