GSM8525076: HCT-WT-24hr-3; Homo sapiens; RNA-Seq
RNA was isolated using QIAshredder columns (Qiagen) and the RNeasy Mini Kit (Qiagen).  On-column DNaseI digestion was performed. Libraries for 3′ mRNA-seq were generated from 100 ng total RNA using the QuantSeq FWD library preparation kit (Lexogen) according to the manufacturer's directions.