Genomic insights into the nuclear and mitochondrial genome of the Caribbean King Crab
A small muscle sample (approx. 2 mm3) was dissected from the first clawed pereiopod and inserted into a sterile 1.5 cm3 sterile centrifuge tube for genomic DNA (gDNA) extraction using the DNeasy Blood and Tissue Kit (Qiagen, Germany) following the manufacturers protocol. gDNA was shipped to Novogene (Palo Alto, California) for next generation library preparation and sequencing (NGS). As detailed in Baeza and Pirro (2024), library preparation was conducted using the Illumina TruSeq kit following the manufacturers instructions and NGS was performed in an Illumina HiSeq X Ten system (Illumina, San Diego, CA, USA) using a 2  150 cycle.