GSM8667512: KO_RNA; Mus musculus; RNA-Seq
Nuclei were isolated according to 10X multiome low cell input nuclei isolation protocol. Briefly, nuclei from 50,000 cells by centrifuging the cell suspension at 300 rcf for 5 minutes at 4°C, followed by resuspension in PBS + 0.04% BSA. After incubating the cells in chilled Lysis Buffer for 4 minutes on ice, Wash Buffer was added without mixing. The sample was centrifuged at 500 rcf for 5 minutes at 4°C, and the supernatant was carefully removed, leaving the nuclei pellet. The pellet was then resuspended in chilled Diluted Nuclei Buffer, and a final centrifugation step was performed. The resulting nuclei were counted, with an expected recovery of about 25% of the initial cell input for sequencing. 10X single nuclei multiome ATAC + 3' RNAseq