sequences of gene
Sampling 1 g of genomic DNA, the sample is randomly fragmented into segments of approximately 350 bp using a Covaris ultrasonic disruptor to construct the library. The entire library preparation is completed through steps including end repair, addition of A-tails, ligation of sequencing adapters, purification, and PCR amplification. After library construction, the integrity of the library fragments and the size of the inserted fragments are assessed using AATI analysis. If the insert size meets expectations, the accurate concentration of the effective library is quantified using Q-PCR (effective library concentration > 3 nM) to ensure the library quality. After the library passes the quality check, different libraries are pooled according to their effective concentrations and target data output requirements, and then subjected to PE150 sequencing.