RNA-seq of barley: Control
RNA extraction from drought-treated and control barley seedling leaves was performed 10 days after the induction of stress, using cumulative leaf samples obtained from three individual seedlings, totaling 90-100 mg of plant material. Total RNA was extracted using the innuPREP Plant RNA Kit (Analytik Jena GmbH, Jena, Germany) according to the manufacturers instructions, and residual genomic DNA was removed with the innuPREP DNase I Digest Kit (Analytik Jena GmbH, Jena, Germany). For transcriptome sequencing, extracted RNA was then stored at 80 C until sequencing at BMKGENE (Mnster, Germany)