Amplicon sequencing of reporter plasmid holding octamer library downstream of SA of the middle exon within a three-exon mini-gene
For the generation of the octamer library, a PCR fragment was generated with a primer containing a random 8-mer. PCR fragments were inserted into the respective backbone resulting in a three-exon mini-gene reporter with an octamer library downstream of the 3ss of the middle exon. The plasmid library was amplified after transformation of E. coli.