GSM8775466: BMM_Cut&Tag; Mus musculus; OTHER
Take out the cryovial and shake it in 37 °C water to quickly thaw it within 1-2 min.Cells were centrifuged for 5 min at 500×g at 4 ℃ Cell activity was detected with LUNA-FL and counted Briefly, the cells are bound to Concanavalin A-coated magnetic beads, and the cell membrane is permeabilized by Digitonin. The enzyme pA-Tn5 Transposase precisely binds the DNA sequence near the target protein under the antibody guidance and results in factor-targeted tagmentation. DNA sequence is tagmented,with adapters added at the same time at both ends, which can be enriched by PCR to form thesequencing-ready libraries(CUT&Tag_Novogene Kit). After the PCR reaction, libraries were.purified with the AMPurebeads and library quality was assessed on the Agilent Bioanalyzer 2100 system.