GSM8773794: A. baumannii 17978ΔMccAB1, stationary, rep 1; Acinetobacter baumannii; RNA-Seq
Total RNA was extracted using the Direct-zol RNA Miniprep Kit (Zymo). RNA was depleted of DNA (Turbo DNAse - Thermo) and ribosomes (QiaSeq Fast Select - Qiagen). 2.7 ug total RNA was used for library construction. RNA libraries for RNA-seq were constructed using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina sequencing according to manuafacturer's protocols.