RNA-Seq of Streptomyces noursei CK-15:wide type
Total RNA was extracted from Streptomyces nourise CK-15 using the TRIzol reagent (Invitrogen) according to the manufacturers protocol. RNA purity and integrity were assessed using a NanoDrop spectrophotometer and Bioanalyzer 2100 (Agilent), ensuring an RNA Integrity Number (RIN) greater than 8. For mRNA-seq library preparation, the mRNA was enriched using the oligo(dT) method (NEBNext Ultra II RNA Library Prep Kit for Illumina, New England Biolabs). The library was constructed following the manufacturer's instructions, with an average insert size of 250 bp. Paired-end sequencing was performed on an Illumina NovaSeq 6000 platform with a read length of 150 bp, yielding approximately 20 million paired-end reads per sample.