genome sequencing of Olios ishikawatodaorum
Genomic DNA was extracted from the cephalothorax and legs of two juvenile specimens (sex unknown) collected from Ibaragabasama, Nagakute-shi, Aichi Prefecture, Japan, 35.178482N,137.084724E, with Genomic-tip 20/G (Qiagen) and sequencing library was prepared using a ligation sequencing kit (SQK-LSK114; Oxford Nanopore Technologies) which was sequenced on the P2 Solo device with a PRO114M flow cell (Oxford Nanopore Technologies). Basecalling was performed using Guppy v.6.4.6 software post-sequencing. For RNA-Seq, total RNA was extracted from the abdomen of a single specimen (identical to one of the specimens used for genomic DNA extraction) using Direct-zol RNA Microprep Kit (Zymo Research) as previously described (Kono et al. 2016), and Illumina library was prepared using the KAPA RNA HyperPrep Kit (KAPA Biosystems) targeting 300-bp fragments. The sequence library was sequenced on a NextSeq 500 (Illumina) with 75 cycles of high-output mode as single-end reads. Sequences were basecalled and demultiplexed with bcl2fastq v.2 software (Illumina).