GSM8612308: D4; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIZOL reagent protocol and then treated with DNase. For the library preparation strategy, 100 ng of total RNA was used as input with the Illumina Stranded mRNA Prep kit, followed by 13 PCR cycles. Sequencing was performed on the NovaSeq6000 platform using an SP-200 Flow Cell, with paired-end reads of 100 cycles each (100x2) and a loading concentration of 0.56 nM