RNA-seq of Schizosaccharomyces pombe: evolved strain G18D08 cycle18 lineD08, h90
RNA was extracted at 0 h and 5 h post nitrogen starvation in MSL-N medium to profile starvation-responsive pathways, with biological triplicates ensuring statistical robustness. Libraries prepared using stranded poly-A selection (NEBNext Ultra II) were sequenced on Illumina NovaSeq platform with paired-end reads (150 bp, 432G).