RNA-Seq of vehicle- vs LPS-treated primary BMDMs from WT or interleukin-1alpha nuclear localisation sequence mutant mice
RNA-Seq of vehicle- vs LPS-treated primary BMDMs from WT or interleukin-1alpha nuclear localisation sequence mutant mice
Primary BMDMs from WT or mNLS mice were treated with vehicle or LPS (1ug/ml, 4h). Supernatant was aspirated, cells washed once with PBS, and then lysis buffer containing 1% mercaptoethanol was added. Total RNA was extracted using a PureLink RNA mini kit. Illumina TruSeq Stranded mRNA kit, according to the manufacturerâ€™s protocol.