The RANK/RANKL axis controls vascular dynamics in the bone marrow
The RANK/RANKL axis controls vascular dynamics in the bone marrow
In accordance with the manufacturer's recommendations, approximately 16,500 live cells per sample were loaded onto the Chromium controller to generate 10,000 single-cell gel-bead emulsions for library preparation and sequencing.  Sinlge cell droplets were generated by 10x Genomics Chromium Controller, and cDNAs with UMIs were generated at single-cell level. Raw FASTQ files obtained from stromal cell populations (Stromal cell 1 and Stromal cell 2; CD45-Ter119-CD31+ and RANKL+CD45-Ter119-CD31+, respectively) were processed using the Cell Ranger pipeline (v6.0.0 or v7.1.0, 10x Genomics) for alignment to the mm10 genome, UMI collapsing, and UMI counting for each gene–cell (barcode) combination.