GSM9447973: SUP-B15_24h_MYCdegr1_rep1; Homo sapiens; ChIP-Seq
ChIP-Seq for H3K27ac was done as described in Boulianne et al using anti-Histone H3 (acetyl K27) antibody – ChIP Grade (ab4729) (Abcam) and polyclonal rabbit IgG (isotype control) (sc2027) (Santa Cruz Biotechnologies), with the exception that initial cross-linking was performed at RT and 1mio cells were used for crosslinking instead of 15mio. ChIP-Seq for MYC (CST #13987, D3N8F) and STAT5 (CST #94205 D206Y) and the IgG control (Abcam #171870) related to these libraries was done with 60mio cells and using double fixation with 2 mM DSG (30min) followed by 1% formaldehyde (30min). ChIP-Seq libraries were generated from ChIP immunoprecipitated DNA using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina (NEB) and sequenced either at Novogene or at the in-house sequencing facility of the MRC London Institute of Medical Sciences at Imperial College London.