Comprehensive Genomic Analysis of Mixta calida Isolated from the Faecal Sample of a Tuberculosis Patient in India
For ONT sequencing, the DNA samples were cleaned and end-repaired using 1X AMPure XP beads (A63880, Beckmann Coulter, USA) and NEBNext UltraTM II End Repair/dA-Tailing Module (E7546, New England Biolabs, MA, USA), respectively. Native barcode ligation was performed with Blunt/TA Ligase Master Mix (M0367, New England Biolabs, MA, USA) using barcodes from SQK-NBD114.96 kit (Oxford Nanopore Technology, UK). Barcoded samples were cleaned with 1X AMPure XP beads and quantified using QubitTM dsDNA HS assay kit (Q32851, Thermo Fisher Scientific, USA) on QubitTM 4 Fluorometer (Q33238, Thermo Fisher Scientific, USA).