GSM9039235: Hippocampus,  sustained hypoxia group (SH) (10% oxygen, 8 hours/day), rep5; Mus musculus; RNA-Seq
Total RNA was extracted from the hippocampi using the RNeasyⓇ Plus Universal Mini Kit (QIAGEN, Tokyo, Japan), according to the manufacturer's protocol. 1 μg of total RNA was used for the construction of sequencing library. cDNA libraries (150-bp × 2 paired-end reads) were prepared using the NEBNextⓇ Poly(A) mRNA Magnetic Isolation Module kit (Cat No. E7490) and NEBNEXTⓇ UltraTMII Directional RNA Library Prep Kit (Cat No. E7760) (New England Biolabs, MA, USA).