single cell RNA-seq of Drosophila melanogaster: adult female Gr59d chemosensory neuron
Full length poly(A)-tailed RNA was used for reverse transcription and amplification using the NEBNext Single Cell / Low Input RNA Library Preparation Kit (New England Biolabs).  To increase cDNA yield, we performed 21 cycles of PCR for single cell samples and 14 cycles of PCR for bulk samples.  Fragmentation was performed for 25 minutes at 37C, then 30 minutes at 65C such that fragments of final library size was between 300-350bp.  Sequencing libraries were prepared using NEBNext Multiplex Illumina Oligos (New England Biolabs).  Single Gr59d chemosensory neurons were sequenced using the Illumina NovaSeq 6000 SP platform using 100bp pair-end reads.