16S amplicon of marine: Northwest Pacific Ocean S30
DNA was extracted using the FastDNA Spin Kit for Soil following the manufacturers protocol. The quality and quantity of extracted DNA were assessed using 1% agarose gel electrophoresis and a NanoDrop 2000 spectrophotometer. The V3V4 hypervariable regions of the bacterial 16S rRNA gene were amplified using primers 338F and 806R in a GeneAmp 9700 thermocycler. PCR products were purified using the AxyPrep DNA Gel Extraction Kit and quantified with a Quantus Fluorometer. Libraries were prepared from the purified products and sequenced on the Illumina MiSeq PE300 platform.