WGS of Mild cognitively impaired human stool sample_24
Whole-genome metagenomic shotgun sequencing was performed as described in our previous study. Briefly, genomic DNA was extracted from ~150 mg of human stool using the QIAamp PowerFecal Pro DNA Kit (Qiagen, USA), following the manufacturers instructions. The DNA was quantified using the Qubit dsDNA HS assay kit (Thermo Fisher Scientific, USA). Approximately 150 ng of quantified DNA was used for library preparation using Illumina DNA Prep (M) Tagmentation kit (Illumina, Inc., 5200 Illumina Way, San Diego, CA, USA) according to the manufacturers instructions. Additionally, sample-specific unique IDT for Illumina-Nextera DNA UD Indexes were used. Sequencing was performed using the Illumina NextSeq1000 and an Illumina NextSeq 1000/2000 P2 Reagents (300 Cycles) v3 reagent cartridge (Illumina, Inc., 5200 Illumina Way, San Diego, CA, USA).