GSM9267379: C2C12 3D-EM, TNF-α 100 ng/mL, 72 h — biol rep 3; Mus musculus; RNA-Seq
Frozen 3D-EM samples were homogenized with a TissueLyser II (Qiagen) using 5-mm zirconia beads at 30 Hz for 15 s ×2. Total RNA was extracted with the miRNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. Libraries were prepared with the TruSeq Stranded mRNA Library Prep Kit (Illumina) and sequenced on a NovaSeq 6000 (Illumina) in paired-end mode (2 × 151 bp).