GSM9589348: LHCNM2, day12-3; Homo sapiens; RNA-Seq
RNA was harvested using Pure Link RNA Mini kit (Invitrogen) RNA libraries were prepared by Novogene. Messenger RNA was isolated using poly-T oligo-attached magnetic beads, followed by cDNA synthesis using random hexamers. Libraries (including strand-specific options using dUTP) were prepared via end-repair, A-tailing, and adapter ligation, then validated using Qubit, real-time PCR, and Bioanalyzer for precise quantification and size distribution.