P2Y14R knockout bone marrow-derived cells stimulated with GM-CSF and FLT3L under UDPG intervention, KO 2, rep 1.
Bone-marrow cells were harvested from 8-12-week-old wild-type (C57BL/6J) or global P2Y14R-knockout mice (P2ry14-/-). cDC2 precursors were then induced with 50 uM 2-mercaptoethanol, 20 ng/mL recombinant mouse GM-CSF, and 100 ng/mL FLT3-L and cultured for 6 days in RPMI-1640 supplemented with 10 % FBS. Cultures were continuously exposed to 100 uM UDPG or vehicle (PBS). The following experimental groups were generated: (1) WT + UDPG, (2) P2ry14-/- + UDPG (n = 3 biological replicates per group).