16S_DNA_Gut_HiLo
Platform: MiSeq; Paired  end: Yes; Read length (bp) : 250; Primer pair: V4: 515F/806R; DNA polymerase : 5 PRIME HotMasterMix ;PCR cycle No. : 35; Adapters:Nextera XT ;Sequencing depth  (avg reads per sample): 617635; Library prep and sequencing protocol: Earthmicrobiome protocol using MiSeq Reagent Kit V3 (600 cycle)