GSM8702903: Retinoic acid-induced N2a cells, DMSO-2; Mus musculus; miRNA-Seq
After extracting the total RNA samples, undergo agarose electrophoresis and Nanodrop quality inspection and quantification, construct a library, use Agilent 2100 Bioanalyzer to measure the library quality, mix the sequencing libraries of different samples, and generate single-stranded DNA through 0.1M NaOH denaturation. According to the supply Commercial Instructions for 50-Cycle Sequencing on the Illumina NextSeq 500 Sequencer Prepare a miRNA sequencing library using total RNA from each sample, including the following steps: 1)3' connector connection: 2) 5' joint connection; 3) cDNA synthesis: 4) PCR amplification: 5) Select 135 ~ 155 bp PCR amplified fragments (corresponding to 15 ~ 35 nt small RNAs): The library was denatured into single-stranded DNA molecules, captured on an Illumina flow cell, amplified in situ into clusters, and according to the supplier's instructions, Sequencing was performed for 50 cycles on an Illumina NextSeq 500 sequencer.