GSM9627651: HA012; Homo sapiens; RNA-Seq
Blood collection and sample preparation. Blood was collected in EDTA collection tubes (Becton Dickinson, USA) within the first 48 hours after birth from preterm neonates (250-300 µl), and at day of life 1 from full-term neonates (500 µl). For healthy adult participants, 1 ml of whole blood was collected via venipuncture. Clinical and research data collection were entered into a web-based electronic case report form created on the REDCap™ platform managed by the University of Florida Clinical and Translational Science Institute. Maternal demographics, indications for delivery, and postnatal data (including demographics) were collected and are outlined in Table 1 and Table S1. Following sample collection, the PBMC was enriched by density centrifugation (Ficoll-PaqueTM PLUS, Cytiva, USA) within 3 hours of blood collection. Library preparation and sequencing. Fresh PBMC suspensions were filtered with a 30-micron MACS® SmartStrainers (Miltenyi Biotec) and resuspended in PBS containing 0.04% bovine serum albumin. After cell counting, samples were adjusted to 1,000 cells/µl and loaded onto the Chromium chips following the 10X Genomics protocol for single-cell 3' kits, version 3 (10X Genomics, USA).