RNA velocity analysis of the PERFORM RNA-Seq dataset
RNA velocity analysis of the PERFORM RNA-Seq dataset
Blood was collected for research purposes at the same time as the first clinically indicated samples (wherever possible), and otherwise within 48 hours of admission. 2.5ml whole blood was collected for RNA expression analysis in PAXgene tubes (for children below 1 year of age, 1ml blood was collected into appropriately reduced volumes of PAXgene fluid) and frozen for later analysis. Total RNA was isolated using PAXgene miRNA blood extraction kits (Qiagen), and then sent for additional DNAse treatment (Zymo Research). Ribodepletion library preparation and RNA-Seq was performed at The Wellcome Centre for Human Genetics in Oxford, United Kingdom.