Transcriptome Raw Reads of Adult Female  Golden Orb-Web Spider, Nephila pilipes, isolate NPFM2
Adult female Nephila pilipes (isolate NPFM2) specimens were collected from a natural habitat and immediately preserved in RNAlater. Dissections were performed under sterile conditions, and total RNA was extracted from pooled tissues including silk glands, cephalothorax, and abdomen using a TRIzol-based method. RNA quality was assessed using a Bioanalyzer (RIN  8.0). Poly(A)+ mRNA was enriched, and strand-specific cDNA libraries were prepared using the Illumina TruSeq RNA Sample Preparation Kit. Sequencing was carried out on an Illumina platform to generate 150 bp paired-end reads. The goal of the study is to investigate tissue-specific gene expression related to silk production, venom components, and metabolic pathways in N. pilipes.