GSM8644735: Total_RNA_HeLa_eIF2A-KO_TM_replica_1; Homo sapiens; RNA-Seq
Total RNA was extracted with Qiagene Rneasy Kit. Ribosomal footprints were extracted from the monosomal fraction after RNaseI digestion via the size selection. Libraries from total RNA samples were prepared using the Illumina TruSeq Stranded library preparation kit. Ribosomal footprints were converted into libraries with the use of Next-Flex small RNA v.4 kit protocol.