GSM8742039: WT pre-chondrocyte cartilage, rep 2; Mus musculus; RNA-Seq
Cells were washed in PBS and scraped in PBS. Cells were pelleted at 200 g for 5 min, supernatant was removed, and samples were processed using the Qiagen RNease Mini kit (Qiagen, 74104), according to the manufacturer's instructions and including the optional DNase digest step. RNA quality and quantity was assessed using a Tapestation 4200 (Agilent). 500 ng total RNA was using to generate RNA libraries using the TruSeq RNA library prep kit (Illumina), according to the manufacturer's instructions.