WT C57BL/6J, PBS day 7, CD11b+ SiglecF- macrophages
This study aimed to characterize transcriptional changes in lung macrophage populations during bleomycin-induced pulmonary fibrosis in mice.  Spp1-EGFP knock-in reporter mice and wild-type C57BL/6J mice were treated with either PBS or bleomycin via intratracheal administration.  Lung macrophages were isolated at multiple time points (days 1, 3, 7, 14, and 28) post-treatment using FACS based on EGFP and surface markers (CD11b, SiglecF).  RNA was extracted and subjected to bulk RNA sequencing using a polyA-selected, strand-specific library preparation on an Illumina NovaSeq 6000 platform.  The data provides insights into macrophage heterogeneity and gene expression dynamics during fibrosis progression.