GSM8585959: ASCs, late control, 14 day, biol rep 2; Canis lupus familiaris; RNA-Seq
The cells directed for RNA isolation were detached from cultures using 1× Trypsin solution and placed in 1mL of TRIzol (Thermo-Fischer Scientific, Waltham, MA, USA). The samples were then frozen at −80°C. The isolation of RNA was conducted using the TRIzol Plus Purification KIT (12183555, Thermo-Fischer Scientific, Waltham, MA, USA) according to the manufacturer's protocol. The cDNA library used for the subsequent analysis was prepared from 100 ng of RNA per sample using TruSeq Stranded mRNA kit (Illumina, San Diego, CA, USA)