Inhibition of A2AR relieves adenosine-mediated suppression of plasma cell differentiation​
Inhibition of A2AR relieves adenosine-mediated suppression of plasma cell differentiation​
Biopsies from 6 cancer patients (head and neck n=1, melanoma n=3, lung n=1, prostate n=1) enrolled in Phase I study IO-001 were processed at screening and C1D21.   Up to five FFPE sections per tissue sample were used, for HE staining and RNA extraction. For each sample a capture area of 6.5 x 6.5 mm (5.000 spots) was annotated by a pathologist. RNA extraction was performed with the High Pure FFPET RNA extraction Kit (Roche). DV200 values were evaluated using BioAnalyzer (Agilent Technologies), and only samples with DV200 values superior to 30% were included in the analysis.  Libraries were prepared using the 10x Genomics PCR-based protocol.