18S DNA of roots for AM fungal metabarcoding
Metabarcoding of 18S DNA. Root DNA was extracted from root samples using Qiagen's DNeasy 96 Plant Kit. For plant mixtures, DNA extracts were pooled at the mesocosm level to ensure equal representation of all species. Subsequently, 18S rDNA metabarcoding was performed using Glom01 primers to amplify a 216 bp fragment of the 18S rDNA region.