GSM8741942: GV oocytes from Control mouse 2; Mus musculus; RNA-Seq
Total RNA was isolated from the GV oocytes without any cumulus cells using the RNeasy Plus Micro Kit (QIAGEN), followed by Agilent 2100 Bioanalyzer RNA 6000 Pico Assay (Agilent Technologies Inc). RNA-seq libraries were prepared using the NEBNext Single Cell/Low Input RNA Library Prep Kit (New England Biolabs) following the manufacturer's protocols. The quantity of library was evaluated by Qubit dsDNA HS Assay (Thermo Fisher Scientific) and the quality was analyzed by the Bioanalyzer (Agilent).