RNA-Seq of Brevitrygon walga: Multi tissue pool
Total RNA was isolated from six distinct tissues collected from a single individual (gill, muscle, heart, liver, spleen, kidney), using 50 mg of each tissue type. After determining RNA concentration, the six RNA extractions were pooled in equimolar concentration. The quality of the RNA pool was assessed on an Agilent Bioanalyzer before normalized random primed cDNA library preparation.