GSM9723411: ATAC-seq NCI-H226 xenograft tumor GNE-8025 0.05 mg/kg rep1; Homo sapiens; ATAC-seq
Tumor tissue was dissociated to a single-cell suspension and 50,000 nuclei were isolated by hypotonic lysis. Nuclei were incubated with Tn5 transposase (Active Motif) for 30 min at 37C to simultaneously fragment chromatin and insert sequencing adapters. ATAC-seq libraries were prepared by Active Motif (order AM46020) following their standard protocol. Transposed DNA was purified and amplified by PCR using NEBNext High-Fidelity 2X PCR Master Mix. Libraries were size-selected for 150-700 bp fragments using SPRI beads and quality-assessed with a Bioanalyzer High Sensitivity DNA chip.