GSM9409668: Pituitary, Str - let7f, rep3; Mus musculus; RNA-Seq
Pituitary glands were homogenized in Trizol reagent (ThermoFisher, 15596026) followed by phase separation with chloroform. RNA isolation proceeded with the aqueous phase using the RNeasy Kit (Qiagen, 75144). Libraries of rRNA-depleted total RNA were prepared with the TruSeq Stranded Total RNA Library Prep (Illumina, 20020596)