GSM8526460: triple negative breast cancer, untreated, rep1; Homo sapiens; RNA-Seq
Total RNA was extracted using the NZY Total RNA Isolation kit (#MB13402, Nzytech), according to manufacturer's instructions. Tumor tissues preserved in RNA later (#AM7021, Thermo Fisher Scientific) at necropsy were transferred to lysis buffer with silica beads (#110791102; Biospec Products), and disrupted in a Minibeadbeater (Biospec Products) for 1-2 min. DNBSEQ Eukaryotic Strand-specific mRNA library