Acinetobacter baumannii (10770_3)
A total of 600ng of purified gDNA from the sample was end-repaired, cleaned up with 1x AmPure beads . Native barcode ligation was performed with NEB blunt/ TA ligase using EXP-NBD104 and cleaned with 1xAmPure beads. Qubit quantified barcode ligated DNA sample was Adapter ligated for 15 minutes using NEBNext Quick Ligation Module. Library was cleaned up using 0.6X AmPure beads and finally sequencing library was eluted in 15 l of elution buffer and used for sequencing.