Sequencing of the V3V4 hypervariable regions of the 16S rRNA gene from adult human stool samples
Library preparation was conducted following the 16S Metagenomic Sequencing Library Preparation Illumina protocol. 16S bacterial rRNA gene was amplified using primers targeting the V3-V4 hypervariable region (341F and 805R primers). Sequencing was performed on a NextSeq1000-Illumina platform (Illumina Inc., San Diego, CA, USA) with a 2 x 300 bp paired-end read length.