GSM8774622: Vehicle_1; Mus musculus; RNA-Seq
RNA-Seq library preparation and sequencing were conducted by Novogene (Beijing, CN) on a NovaSeq 6000 machine for IECs (EpCAM+CD45-) from wild type pups were sorted via BD FACS) ( pretreated with vehicle or PEox) The end reading of 150bp pairing was generated. RNA-Seq fastq files were first processed by fastp to remove adaptor and low-quality reads, then aligned to the reference genome using Hisat2 (v2.0.5).