GSM8773875: Dauer Day15, exit 6h, sample 3; Caenorhabditis elegans; RNA-Seq
Each condition contained three biological replicates of 8000 worms, collected in ice-cold 1x M9 buffer from plates seeded with OP50. The samples were washed three times with 1x M9 buffer, resuspended in Trizol (Invitrogen) and 0.7 mm zirconia beads (Roth), and quick-frozen in liquid nitrogen at -80°C until RNA extraction was performed. The samples were disrupted using a Precellys24 homogenizer (Bertin) and 1-Bromo-3-chloropane (Sigma-Aldrich) was added for phase separation. The purification of RNA was performed using the RNeasy Mini Kit (Qiagen) according to the provided instructions. The isolated RNA was eluted in RNase-free H2O and quantified using a NanoDrop 8000 spectrophotometer (Thermo Fisher Scientific).