GSM8744826: TBI_m6A_0; Mus musculus; OTHER
Total RNA was extracted from the cerebral cortex of mice using TRIzol reagent. After cell lysis, chloroform was added and centrifuged to separate the layers. The upper aqueous phase was collected, and isopropanol was added to precipitate RNA, followed by a wash with 70% ethanol. RNA libraries were constructed using an Illumina library preparation kit, synthesizing cDNA from m6A-modified RNA in the samples, followed by adapter ligation and PCR amplification to obtain the library.