Genomic nanopore sequencing of a clonal isolate of Acanthamoeba castellanii Neff transfected with pGAPDH-EGFP - ('Clone 1')
Genomic DNA was extracted from an axenic monoclonal culture of pGAPDH-EGFP-transfected Acanthamoeba castellanii Neff (ATCC-30010) using an SDS-based lysis and a phenol-chloroform-based extraction method. DNA was cleaned using a QIAgen G/20 Genomic Clean-up Column. A library was prepared with the Ligation Sequencing Kit (SQK-LSK109) and barcoded with the Native Barcoding Kit (EXP-NBD104). Barcode 2 was used for this sample. DNA was not sheared. Raw reads were basecalled with Albacore 2.3.3, adaptors were removed with Porechop.