qiita_ptid_18071:15900.s6.S6
timecurse_TCDD_treatment
DNA of cecal and fecal samples were extracted using QIAamp PowerFecal DNA Kit (QIAGEN) according to manufacturers instructions and sequenced for the V4 region of the 16S rRNA amplicon, performed with Illumina MiSeq technique. The 16S rRNA sequencing targeting the V4 region was performed using the Illumina MiSeq Reagent Kit (Illumina, MS102-2001). For amplicon sequencing the libraries were prepared following the Illumina 16S Library preparation protocol, amplifying the variable V4 regions of 16S rRNA (FWD: GTGYCAGCMGCCGCGGTAA; REV: GGACTACNVGGGTWTCTAAT) and using Illumina TruSeq HT Kits i5 and i7 barcodes. Sequencing was performed in single-end at 285 bp in the Illumina MiSeq using a v2 cassette, 30 percent DNA of PhiX was added to the library to avoid low complexity sequencing issues.