Nanopore & Illumina sequencing of Vibrio navarrensis W2D
Genomic DNA was extracted using an optimized SDS-based protocol (Oxford Nanopore Technologies). Libraries were prepared using SQK-LSK110 kit (Oxford Nanopore Technologies) for long-read sequencing and VAHTS Universal Plus DNA Library Prep Kit (Vazyme) for short-read sequencing. Sequencing was performed on Nanopore PromethION and Illumina NovaSeq 6000 platforms. Raw reads were quality-filtered (Nanopore: Q7, length1600 bp; Illumina: fastp). Assembly was performed using Unicycler.