GSM8493703: peptide_stimulated_CD8,scRNAseq; Mus musculus; RNA-Seq
CD8+ T cells from mice immunized with the self-reactive peptides mix were labeled with CellTrace Violet (CTV) dye and restimulated with the self-peptides in vitro.The CD44+ CTVlow CD8+ T cells were sorted by flow cytometry for single-cell sequencing. CD44+CTVlowCD8+ T cells were sorted from peptides stimulated lymphocytes and utilized to prepare 5' gene expression (GEX) and paired T cell receptor (TCR) libraries using the 5' Chromium Next GEM Single Cell v2 (Dual Index) reagents and protocol provided by 10X Genomics