GSM8743999: SC5314 adr1ΔΔ, bio rep2, acetate; Candida albicans; RNA-Seq
RNA extraction was done by disrupting cells with Zirconia beads (Ambion, Fisher Scientific, Waltham) and isolating RNA using 25:24:1 phenol:chloroform:isoamyl alcohol in combination with a Qiagen RNeasy Mini Kit Sequencing libraries were generated by purifying messenger RNA from total RNA using poly-T oligo–attached magnetic beads. The first cDNA was synthesized using random hexamer primers, followed by sequencing of both ends of cDNA fragments using the Illumina platform