SW-P-R1-1.4S
Glomeromycota 18S rRNA  amplicons generated in two PCR rounds: first with AML2-NS31 primers, then with custom-barcoded AMV4.5F-AMDGR. Standard barcoded adaptors were ligated to pools of amplicons. Libraries were sequenced on NovaSeq 6000 using 500 cycle kit v1.5.