ATAC sequencing of untreated and LLOMe treated MEF cells
ATAC sequencing of untreated and LLOMe treated MEF cells
Untreated and treated MEFs (50,000 cells) are collected in duplicates from different plates of each group and subjected to Tagmentation using Tn5 (Zymo-seq ATAC Library kit: D5458) The Tagmented DNA was isolated using the DNA purification columns provided in the Zymo kit.  The ATACseq library was PCR amplified using the Unique Dual Index Nextera primers. Amplified libraries are purified using ampure beads.