GSM8773453: Parental LU65, SR-4835, biol rep 1; Homo sapiens; RNA-Seq
Homogenized lysates from three independent replicates were thawed and extracted using the RNeasy Mini kit (Qiagen) with on-column DNase I digestion (Qiagen) according to the manufacturer's protocol. RNA content was quantified using a Qubit fluorometer, and 100 ng of total RNA was used to generate cDNA using the NuGEN Universal RNA-Seq Library Preparation Kit (Tecan Genomics). Quality control on prepared libraries was performed by TapeStation size assessment and quantification using the Kapa Library Quantification Kit (Roche).