16S_DNA_Gut_HiLo
Platform: MiSeq; Paired  end: Yes; Read length (bp) : 250; Primer pair: V4: 515F/806R; DNA polymerase : ThermoFisher Platinum Hot Start;PCR cycle No. : 35; Adapters:Nextera Flex;Sequencing depth  (avg reads per sample): 316174; Library prep and sequencing protocol: Illumina 16S Metagenomic Sequencing Library guide (v. 15044223-B)