GSM8743866: Naive_Ast_2; Mus musculus; RNA-Seq
Brain cell suspensions were prepared, and oligodendrocytes and astrocytes were isolated using magnetic-activated cell sorting (MACS) with cell type-specific MicroBeads: oligodendrocyte: Anti-O4 MicroBeads, astrocyte: Anti-ACSA-2 (astrocyte cell surface antigen-2) MicroBeads. RNA extraction was performed using the RNeasy Plus Micro Kit following the manufacturer's protocol. RNA quality was assessed using the Bioanalyzer 2100 system (Agilent Technologies), and all RNA samples had a RNA integrity number (RIN) greater than 6.8. The cDNA library was prepared using the NEBNext Ultra Directional RNA Library Prep Kit (New England BioLabs).