Genomic Illumina sequencing of a clonal isolate of Acanthamoeba castellanii Neff transfected with linearized pGAPDH-EGFP - ('Clone LT9')
Genomic DNA was extracted from an axenic liquid culture of Acanthamoeba castellanii Neff (ATCC-30010) using an SDS-based lysis and a phenol-chloroform-based extraction method. A library was prepared, barcoded, and sequenced on the Illumina NextSeq 2000 instrument with 150 bp paired end reads.