Amplicon sequencing of Vitis vinifera: rhizoplane at veraison stage
Metabarcoding libraries were prepared using the Herculase II Fusion DNA Polymerase Nextera XT Index V2 Kit targeting the V3-V4 regions of bacterial 16S rRNA with primers 337F (5-GACTCCTACGGGAGGCWGCAG-3) and 800R (5-TACCAGGGTATCTAATCC-3).