RNA-seq of Chrysomya bezziana pooled eggs, larvae, females, and males
We isolated whole body RNA using Direct-zolTM RNA MiniPrep Plus with TRI Reagent (ZymoResearch, R2073) and Quick-RNA Miniprep Kit (R1055). Each sample had its RNA isolated separately and then pooled in equimolar aliquot. The  CollibriTM Stranded RNA Library Prep Kit (A38996096) was used for library construction. All samples underwent paired-end sequencing on the Illumina (San Diego, CA, USA) NextSeq 500 platform.