GSM8615128: Day 2; Mus musculus; Bisulfite-Seq
Genomic DNA was purified from cell lysates using Zymo Genomic DNA Clean & Concentrator-10 Kit. 500 ng of starting input genomic DNA was digested with 30 units of MspI (NEB). Fragments were ligated to pre-annealed adapters containing 5'-methyl-cytosine instead of cytosine according to Illumina's specified guidelines. Adaptor-ligated fragments ≥50 bp in size were recovered using the Zymo DNA Clean & Concentrator™-5. The fragments were then bisulfite-treated using the Zymo EZ DNA Methylation-Lightning™ Kit. Preparative-scale PCR was performed and the resulting products were purified with Zymo DNA Clean & Concentrator™-5 for sequencing on an Illumina NovaSeq 6000 platform.