Pseudomonas aeruginosa 6717097
Whole genomic DNA was extracted from a loop of overnight colonies re-suspended in 100 L phosphate-buffered saline. A total of 90 L Bacterial Lysis Buffer and 10 L Proteinase K (Qiagen GmbH, Germany) were added, incubated for 10 min at 65C then 10 min at 95C, and high molecular weight DNA was isolated using the MagLEAD 12gC (PSS co.) and MagDEA DX Sv Kit, according to the manufacturers instructions. The DNA was sent to Plasmidsaurus (plasmidsaurus.com) for Oxford nanopore sequencing.