GSM8615156: 1321N1_Cd_24h_rep1; Homo sapiens; RNA-Seq
Total RNA was isolated using the QIAGEN RNeasy kit including the on-column DNase I digestion. RNA-sequencing libraries were generated following the QuantSeq 3'- mRNA-Seq FWD library preparation protocol (Lexogen). Library concentrations were quantified with the Qubit 2.0 Fluorometric Quantitation system (Life Technologies) and the size distribution was assessed using the 2100 Bioanalyzer instrument (Agilent). QuantSeq 3'-mRNA sequencing (Lexogen)