WGS of Pseudomonas aeruginosa bloodstream infection
DNA extraction with the EZ1 Advanced XL system (Qiagen, Hilden, Germany) according to the manufacture instructions. The quantity of the extracted DNA was measured using a Qubit 3.0 fluorometer with the double-stranded DNA (dsDNA) assay kit (Thermo Fischer Scientific, MA, USA). Extracted DNA was diluted to an approximate target sample concentration of 10 nanogram/microliters and a target sample volume of 50 microliters