Longread (CCS) sequencing by Sequel II
Genomic DNA was sheared with Diagenode's Megaruptor 2 system. SMRTbell express template prep kit 2.0 (PacBio) was used to prepare SMRTbell libraries according to the manufacturer's protocol. The libraries were size-selected with the BluePippin system (Sage Science), which removed DNA fragments shorter than 10 kb.