Illumina PE of Somniosus pacificus: skeletal muscle
Pacific sleeper shark S. pacificus were collected from Suruga Bay, Japan, in May 2019 and subsequently preserved at -4C. DNA was extracted from the skeletal muscle of the trunk region using the DNeasy Blood & Tissue Kit (Qiagen). The extracted DNA was fragmented using the NEBNext dsDNA Fragmentase (New England Biolabs) and then prepared for sequencing with the Nextera DNA Library Preparation Kit (Illumina). Sequencing was performed on the Illumina HiSeq X platform.