GSM9589992: H660 shControl replicate 1; Homo sapiens; RNA-Seq
Total RNA was extracted from cultured cells using TRIzol reagent following the manufacturer's instructions. RNA quality and concentration were assessed using spectrophotometry and agarose gel electrophoresis prior to library preparation. RNA sequencing libraries were prepared using a poly(A) mRNA enrichment strategy followed by cDNA synthesis and adapter ligation according to the manufacturer's protocol. Libraries were amplified and sequenced on an Illumina platform to generate paired-end sequencing reads.