GSM9037618: Standard chow scRNA-seq; Mus musculus; RNA-Seq
For the Parse Biosciences platform, the cells were prepared using the Fixation Kit in Evercode™ WT Mini v3 package (Parse Biosciences, Seattle, WA, USA) according to the manufacturer's protocol. After fixation, the cell count was determined using the TC20™ Automated Cell Counter (Sigma Aldrich). A total of 20,000 cells from dissociated tumor of control diet- and ketogenic diet-fed NSG mice was used, resulting in an average of approximately 10,000 cells per tumor. These cells were subjected to the Single Cell Whole Transcriptome Kit v2 (Parse Biosciences, Seattle, WA, USA) for library construction