Low rice tiller number F2 No.L5
Genomic DNA of selected individuals was isolated using modified CTAB method . As phenotypic distribution was closed to normal, we collected leaf of 30 F2 plants on each ends. DNA-seq libraries were made from genomic DNA as well as that of two parents and sequenced the whole genome using DNBSEQ DNBSEQ-T7 to generate paired end read with a sequence depth of about 20x of the rice genome (~400 Mb) for each line