Infected dog5
The PCR reaction system was configured with 30ng qualified genomic DNA samples and corresponding fusion primers, and the PCR reaction parameters were set for PCR amplification. Agencourt AMPure XP magnetic beads were used to purify the PCR amplification products and dissolve them in Elution Buffer. Labels were attached to complete the library construction. The fragment range and concentration of the library were measured using Agilent 2100 Bioanalyzer. Qualified libraries were sequenced using sequencers according to the size of the inserted fragment.