GSM9267554: HOB cultured on no coating metal day21_3; Homo sapiens; RNA-Seq
Osteoblasts RNA was purified using TRIzol Reagent and RNA extraction kit (NucleoSpin®) according to the manufacturere's protocol The libraries were generated from high-quality RNA (1 μg) of each sample using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs, Ipswich, MA, USA) and then subjected for sequencing (Illumina NovaSeq 6000, Illumina, Inc. San Diego, CA, USA). The sequence data of 150 bp × 2 paired ends of 6G bases/sample (3 samples for each condition) were trimmed and mapped via HISAT2 v2.0.5. Reads were counted (FeatureCount v1.55.0-p3) and the number of fragments per 1 million bases of the transcription sequence of each gene was calculated based on the gene and read length. Significance was set up using the Benjamini-Hochberg approach as p <0.05 for false discovery rate (FDR) . The gene ontology and pathway enrichment analyses and GSEA were performed on the differential expression data using NovoMagic online tools (Novogene, Singapore).