16S rRNA sequencing of microalgae-associated microbiota culture-derived samples
DNA was extracted at the end of bioreactor cultivation. Extracted total DNA was amplified using a dual-stage PCR targeting near-full lenght 16S (V1V8) rRNA region for prokaryotic communities. Barcoding was performed in a second PCR step prior to sequencing on an Oxford Nanopore GridIONx5 platform.