Shotgun metagenomic sequencing of metagenome: biological soil crusts from the Barberton Greenstone Belt (Geology = Serpentinite)
Genomic DNA extracted from biological soil crust samples was used for shotgun metagenomic library preparation. Libraries were constructed with an average insert size of approximately 350 bp and sequenced on the Illumina NovaSeq 6000 platform using a paired-end 2 x 150 bp strategy. Sequencing was performed by NovogeneAIT Genomics (Singapore). Initial quality control was conducted by the sequencing provider, and reads with base quality scores less than or equal to Q20 were removed prior to data delivery.