GSM9514661: ATAC-Seq - RorccreAtg5fl/fl_CKO2_2; Mus musculus; ATAC-seq
Cell samples were obtained and nuclei were extracted. Tn5 transposase was added to the nuclear suspension to perform the transposition reaction. After the reaction was completed, DNA fragments were purified; subsequently, PCR amplification was carried out, and the fragments were purified using AMPure XP beads (Beckman Coulter, Brea, CA, USA).Sequencing libraries were constructed. Upon completion of library construction, the libraries were subjected to quality inspection using an Agilent 2100 (Agilent, Santa Clara, CA).Libraries that passed the quality inspection were used for on-machine sequencing (NovaSeq X Plus) to obtain sequence information of the open chromatin region fragments to be tested.