Acinetobacter baumannii (10770_3)
Library construction was carried using QIASeq FX DNA LibraryPreparation protocol. 50 ng of Qubit quantified DNA was enzymatically fragmented, end-repaired and A-tailed in a one-tube reaction using the FX Enzyme Mix provided in the QIASeq FX DNA kit. The end-repaired and adenylated fragments were subjected to adapter ligation, whereby index-incorporated Illumina adapter was ligated, to generate sequencing library. The library was subjected to 6 cycles of Indexing-PCR (Initial Denaturation at 98C for 2 min, cycling (98C for 20sec, 60C for 30sec, 72C for 30sec) and final extension at 72C for 1min) to enrich the adapter-tagged fragments. Finally, the amplified libraries were purified using JetSeq Beads and quantified by Qubit fluorometer and the fragment size distribution of the final sequencing library pool was analyzed on Agilent 2200 TapeStation.