GSM8667739: NE 72h WT replicate 2; Homo sapiens; RNA-Seq
Total RNA was isolated from cell pellets using Quick-RNA Miniprep kits (ZYMO Research, R1055) per the manufacturer's instructions, including the digestion and removal of DNA. After RiboGreen quantification and quality control by Agilent BioAnalyzer, 100-500 ng of total RNA with RIN values of 9.4-10 underwent polyA selection and TruSeq library preparation according to instructions provided by Illumina (TruSeq Stranded mRNA LT Kit; RS-122-2102). Samples were amplified by 8 cycles of PCR, barcoded, and run on a NovaSeq 6000 platform in a PE100 run, using the NovaSeq 6000 S2 Reagent Kit (200 Cycles) (Illumina).