GSM9405281: AP3; Mus musculus; RNA-Seq
Lysis: Homogenize samples in TRIzol® (tissue/cells) for complete RNA release. Phase Separation: Add chloroform, centrifuge to isolate RNA in the aqueous phase. Precipitation: Mix with isopropanol, incubate, and centrifuge to pellet RNA. Wash: Remove contaminants with 75% ethanol. Column Purification: Use silica columns (e.g., Zymo Kit) for DNase treatment and high-purity RNA elution. RNA-seq libraries were constructed using the VAHTS Universal V6 RNA-seq Library Prep Kit. For sequencing and analysis