GSM9514720: VD2; Mus musculus; RNA-Seq
Brains were perfused with ice-cold saline and freshly harvested at 42 days after tMCAO. Single live cells were separated into Gel bead in emulsion (GEM)s formed by oil micro-droplets by the Chromium instrument (10X Genomics) using Single Cell 3' v3 reagents. Each GEM contains a gel bead and a cell. A greater than 1000-fold excess of partitions compared to cells assures that most GEMs were only one cell/GEM. The reaction mixture/emulsion with captured and barcoded mRNAs were removed from the Chromium instrument followed by reverse transcription. The cDNA samples were fragmented and amplified per 10X protocol. The libraries were then purified, quantified, and sequenced on an Illumina HiSeq.