GSM8773407: WT_BS_CC30_GFPneg_rep1; Mus musculus; RNA-Seq
To perform RNA-seq, we FAN-sorted neurons from hippocapi and brainstems at different time points after intravenous wtLCMV challenge. Each biological replicate represents 50000  neuronal nuclei sorted in buffer RLT with 1% ß-mercaptoethanol and processed with RNeasy plus Micro Kit (Qiagen) following the manufacturer's instructions. RNA libraries were prepared for sequencing using standard Illumina protocols