GSM8640714: ATAC-Seq, epithelium, control, rep 2; Homo; ATAC-seq
Firstly, cell nuclei were isolated. The specific procedures were as follows: initially, a complete and uniform cell suspension was obtained. Subsequently, 50,000 cells were counted and centrifuged at 500 ×g for 5 minutes at 4°C centrifuge. The pellet was washed with 50 μl cold 1*PBS buffer and centrifuged again at 500 ×g for 5 minutes at 4°C. After discarding the supernatant, 50 μl of pre-chilled nuclear lysis buffer was added, followed by slow pipetting to resuspend the pellet. The mixture was then centrifuged at 500 ×g for 5 minutes at 4°C. The denatured and diluted libraries were loaded onto the FlowCell at a specific concentration based on the expected sequencing data volume. The libraries were hybridized with adapters on the FlowCell and underwent bridge PCR amplification on the cBot cluster generation platform, followed by sequencing on the sequencer.