GSM8978167: LM23 PBMCs post-treatment with Abatacept; Homo sapiens; RNA-Seq
Cryopreserved PBMCs before and after abatacept treatment from patients CM11 and LM23 were thawed and diluted in complete RPMI1640 medium and centrifuged at 300g for 5 minutes. The pellet was resuspended and counted before being pelleted again and suspended at a density between 700-1200 cells/μL in sterile PBS with 0.04% BSA before being loaded into a Chromium Next GEM Chip G using the Chromium Controller. Library preparation was done using the Chromium Single Cell 3' Reagent Kits User Guide (v3.1 - Dual Index) (10X Genomics, Pleasanton, CA) according to manufactorer recommendations. Sequencing was performed using Novoseq X plus.