GSM9414359: Visium FFPE, T1190_RCA; Homo sapiens; OTHER
Visium FFPE: Fresh coronary tissue was fixed in 4% PFA overnight, washed with PBS, and embedded in FFPE blocks. FFPE blocks were sectioned onto Visium slides and underwent H&E staining before imaging.  Libraries were generated using the Visium FFPE v1 kit (#1000188; #1000362; #1000364) and sequenced on a NextSeq2000 according to manufacturer's recommendations. Libraries were sequenced on a NovaSeq 6000. Xenium in situ: A custom gene panel was designed (Supplementary Table 4) based on the CITE-seq reference map and combined with the Xenium Human Multi-Tissue and Cancer Panel (#1000626, 10x Genomics). FFPE blocks and sectioned and the 10x Genomics Xenium In Situ Gene Expression with Cell Segmentation Staining User Guide protocol (10x Genomics, Protocol number CG000749) used for sample (#1000661; #1000460; #1000487. 10x Genomics,). Visium FFPE: Fresh coronary tissue was fixed in 4% PFA overnight, washed with PBS, and embedded in FFPE blocks. FFPE blocks were sectioned onto Visium slides and underwent H&E staining before imaging.  Libraries were generated using the Visium FFPE v1 kit (#1000188; #1000362; #1000364) and sequenced on a NextSeq2000 according to manufacturer's recommendations. Libraries were sequenced on a NovaSeq 6000. Xenium in situ: A custom gene panel was designed (Supplementary Table 4) based on the CITE-seq reference map and combined with the Xenium Human Multi-Tissue and Cancer Panel (#1000626, 10x Genomics). FFPE blocks and sectioned and the 10x Genomics Xenium In Situ Gene Expression with Cell Segmentation Staining User Guide protocol (10x Genomics, Protocol number CG000749) used for sample (#1000661; #1000460; #1000487. 10x Genomics,).