Long-read native whole genome sequencing of Bacteroides uniformis isolated from the WildR mouse gut microbiome
High-molecular weight gDNA was extracted from cell pellets using the Qiagen Genomic Tip (20/G). Libraries were prepared with the Oxford Nanopore SQK LSK-110 kit to repair DNA. Then barcodes were ligated on with the reagents compatible with the SQK LSK-109 kit. Finally, adapter sequences were ligated on as described in the LSK-110 kit. The sequencing library was loaded onto a R.9.4.1 SpotON flow cell and run on the Mk1C until  200,000 reads were collected for each sample.