DNA-Seq of Hainan gibbon: museum sample
Libraries were treated with uracil-DNA glycosylase (UDG) and Endonuclease VIII to remove ancient-DNA deamination damage prior to amplification. PCR was performed using AccuPrime Pfx DNA polymerase, with sample-specific indexes incorporated into P5 and P7 adapters. Libraries were quantified by NanoDrop and Agilent Bioanalyzer before sequencing.