GSM9088582: Primary neurons,shKIF3A,Day5 rep3; Mus musculus; RNA-Seq
Total RNA was extracted by the trizol method Messenger RNAwaspurified from total RNA using poly-T oligo-attached  magnetic beads. After fragmentation, the first strand cDNA was  synthesized using random hexamer primers. Then the second strand  cDNA was synthesized using dUTP, instead of dTTP. The directional  library was ready after end repair, A-tailing, adapter ligation, size  selection, USER enzyme digestion, amplification, and purification. The  library was checked with Qubit and real-time PCR for quantification and  bioanalyzer for size distribution detection