whole-genome resequencing of grizzly bear samples from British Columbia, Canada
Genomic DNA was extracted from hide tissue using column extraction (QIAGEN) from 25 mg slivers of dired tissue. Purified genomic DNA was quantified then submitted to Genome Quebec Innovation Centre for PCR-free shotgun whole-genome library preparation to be sequenced on HiSeqX and NovaSeq6000 S4 (illumina) using paired-end 150 bp reads to a per-sample target depth of 10X coverage.