RNA-seq of Mus musculus: distal colon
The quality of isolated RNA was determined using a Bioanalyzer (Agilent) with minimum acceptable RIN value of 8. cDNA was prepared using the TruSeq Stranded Total RNA Sample Preparation kit with Ribo Zero Gold (Rev. E; Illumina) according to manufacturer's protocol, and sequenced using a NovaSeq 6000 platform (Illumina)