RNA-Seq of Homo sapiens esophageal cancer cell KYSE450 xenograft after treatment with gefitinib
RNA was extracted from the human esophageal cancer cell KYSE450 xenograft after treatment with gefitinib in nude mouse, and mRNA was enriched from qualified RNA sample by using magnetic beads containing Oligo (dT). The first strand of cDNA was synthesized using randomly interrupted mRNA as template, and random hexamers as primers, followed by synthesis of the second strand of cDNA by PCR. Double strand cDNA was purified using AMPureXP beads, and then was subjected to terminal repair, addition of an A tail and ligation of sequencing adapters. PCR enrichment was performed to obtain the final cDNA library.