Mus musculus; liver; control; biological replicate P
extraction of RNA; oligo dT beads used to enrich mRNA with poly A tail; mRNA molecules fragmented into small pieces; fragmented mRNA synthesized into first strand cDNA using random primers; second strand cDNA  synthesized with dUTP; synthesized cDNA subjected to end-repair and 3' adenylated - adaptors were ligated to the ends of these 3' adenylated cDNA fragments; digestion of the U-labeled second-strand template with Uracil-DNA-Glycosylase (UDG);  PCR amplification; library quality control; library circularization; library  amplification to make DNA nanoball (DNB); sequencing on DNBSEQ (DNBSEQ Technology) platform; reads cleaned to filter out low-quality sequences, adaptor sequences and contamination.