Whole genome sequencing of LSDV isolated in 2025 from Cattle in Tunisia
300ng of DNA was used to assess the quality and size distribution using Agilent Genomic DNA ScreenTape on Tapestation 2200. SMRTbell libraries were prepared with the SMRTbell prep kit 3.0, which includes shearing, repair, A-tailing, ligation of barcodes and adapters, and size selection with SMRTbell clean-up beads. Pooled libraries were sequenced on the PacBio Sequel IIe platform under CCS mode for 30 hours