Pooled sample, PacBio long reads
cDNA conversion and amplification was conducted using the NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module according to the manufacturers instructions. cDNA was size selected for 2kb length transcripts and PacBio libraries were prepared with the SMRTbell Template Prep kit 2.0. Qubit RNA BR Assay kit and subsequent analysis on a Qubit 4.0 Fluorometer and (ThermoScientific) was used to quantify the library and the quality was assessed using a TapeStation System. Sequencing primer v4. Binding kit 2.1/control 1.0 was used to prepare the library for sequencing.