Shotgun sequencing of EBPR performing enrichment culture
DNA from the biomass samples was extracted using the DNeasy PowerSoil Pro-Kit (Qiagen, Germany) following the manufacturer's protocol. Shotgun sequencing was performed by Hologenomix (Delft, Netherlands). Paired-end sequencing with a read length of 150 bp was conducted using the Illumina NovaSeq X sequencing system. Library preparation was carried out using the Nextera XT DNA Library Preparation Kit. Approximately 10 Gbp of sequencing data were generated per sample.