GSM9150090: Lactobacillus delbrueckii subsp. bulgaricus cells,CT; Lactobacillus delbrueckii subsp. bulgaricus; RNA-Seq
The L. bulgaricus solution cultured at 37 degrees Celsius for 13 hours in normal MRS medium was used as the control group, and after 13 hours, 0.64mg/ml rifampicin was added and treated for 3 hours to form the experimental group. Using M20 Genomics VITA series MscRNA seq, prepare a single-cell suspension and immobilize the cells, then add random primers into the single cells. Afterwards, reverse transcription was performed using random primers as the amplification starting point to obtain random RNA segments. Finally, Gel Beads with sequence labels, pre mixed samples and reagents, and oil were loaded into their respective injection channels for cell labeling to obtain a library of single-cell cell markers for microorganisms. Finally, cDNA amplification was performed on the single-cell library to obtain Illumina based library for sequencing.（n=1）