RNA seq of Halisarca dujardinii: intact tissues, specimen 1
Samples were lysed in ExtractRNA (Evrogen, BC032). RNA extraction was performed using ExtractRNA (Evrogen, BC032) followed by purification with CleanRNA Standard kit (Evrogen, BC033). Resulting samples were checked for RNA integrity by electrophoresis in 1% agarose gel in Tris-acetate-EDTA buffer (Evrogen, PB022). Purified RNA samples in RNAse-free water were freezed and stored at -80C until library preparation. Polyadenylated fraction of total RNA was purified with NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Libraries were constructed with NEBNext Ultra II RNA Library Prep Kit and sequenced on the Illumina NovaSeq6000 at paired-end mode with 150 bp read length.