GSM9415286: NT rep2; Homo sapiens; OTHER
Okazaki fragments were separated from bulk gDNA by sucrose gradient centrifugation, followed by streptavidin affinity capture of biotinylated, EdU-labelled DNA. Purified fragments were treated to remove 5′ RNA primers, phosphorylated at the 5′ termini, ligated to sequencing adapters on both ends, and amplified with indexed primers.