RNA-seq of Saccharina japonica
Eukaryotic RNA-seq libraries were prepared using the Illumina Stranded mRNA Prep, Ligation kit (San Diego, USA), wherein mRNA was isolated with oligo(dT) magnetic beads and reverse-transcribed into cDNA using random primers. The synthesized cDNA was subsequently subjected to end repair, adenylation, and adapter ligation according to the manufacturers protocol. Library fragments of 300400 bp were size-selected using magnetic beads and amplified with 1015 cycles of PCR. Sequencing was carried out on the NovaSeq X Plus platform (PE150) using NovaSeq reagent kit.