GSM8808779: Muscle,Intermittent_fasting,Male,rep7; Mus musculus; RNA-Seq
RNA was extracted using TRIzol Reagent. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library.For the directional library, it was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification