GSM8808664: Preconditioning 2; Homo sapiens; RNA-Seq
total RNA was extracted from cells using the RNeasy Fibrous Tissue Mini Kit (Qiagen) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, thefirst strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis usingeither dUTP for directional library or dTTP for non-directional library.