GSM9414984: IFT20 siRNA / IFT88 siRNA pool rep 2.1; Homo sapiens; RNA-Seq
Total RNA was harvested 72 hrs post transfection using TRIzol (Thermo Fischer Scientific), following standard manufacturers protocol. High quality RNA samples were DNAse treated according to manufacturers protocol (#AM1907, TURBO DNA-freeTM Kit) prior to library preparation. Total RNA was treated with RNAseH and strand-specific libraries prepared and sequenced using a single-end 75 cycle high output kit on an Illumina NextSeq500 at the ACRF Cancer Genomics Facility.