GSM8808534: 35-day-old Arabidopsis rosettes exogenously mock treated with 0.01 % DMSO and 0.1 % ethanol; Arabidopsis thaliana; RNA-Seq
Nuclei isolation was performed upon whole rosettes. Frozen tissue was crushed using a mortar and pestle, and nuclei released from homogenate using a resuspension buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 1% PBS, 1% Superase RNAse Inhibitor). The resulting homogenate was filtered using a 30mm filter. To enrich for nuclei, an Optiprep (Sigma) gradient was employed. Enriched nuclei were then purified using Fluorescent Activated Cell Sorting (FACS). Purified nuclei were loaded directly onto the 10X machine 10X-Gene Expression v3.0 chemistry and sequenced on the Illumina Novaseq 6000 with 150 bp paired end chemistry.