RNA sequencing of Mus musculus: 4T1 PD-L1 overexpressed tumor treated with IgG (Sample 2)
Total RNA was extracted from 4T1 mammary tumors of BALB/c mice. The tumors were derived from 4T1 control cells or PD-L1 overexpressing cells, and treated with IgG or anti-PD-L1 antibodies. Poly-A selection was performed to enrich mRNA, and cDNA libraries were prepared using an Illumina-compatible kit. Paired-end sequencing was performed on the Illumina NovaSeq 6000 platform.