GSM9149849: mouse Morula embryo - TMZ2; Mus musculus; OTHER
Cells were bound to concanavalin A-coated magnetic beads and permeabilized with the nonionic detergent digitonin ChIP-seq libraries were prepared using Hyperactive® Universal CUT&Tag Assay Kit for Illumina.By using a primary antibody against H3K18ac, along with a corresponding secondary antibody and Protein A/G, the transposase fused to Protein A/G is precisely directed to cleave DNA sequences near the target protein. During the cleavage process, adaptor sequences are simultaneously added to both ends of the DNA fragments. The fragments were then amplified by 18 cycles of PCR to generate libraries suitable for high-throughput sequencing.