GSM9150414: Legionella pneumophila subsp. pneumophila strain Philadelphia 1, control, rep_1; Legionella pneumophila subsp. pneumophila str. Philadelphia 1; RNA-Seq
total RNA was prepared using the Maxwell RSC Simply RNA Tissue Kit (Promega). Following rRNA removal with the NEBNext rRNA Depletion Kit (New England BioLabs) the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs) was used with 20 ng of total RNA for library construction.