Rhizosphere soil of ryegrass (control rep3): 16S rRNA gene amplicon
Total DNA was extracted from soil. The V3-V4 region of bacterial 16S rRNA gene (or ITS1 region of fungal ITS) was amplified using specific primers (e.g., 338F/806R). PCR products were purified and prepared for sequencing on the Illumina MiSeq platform.