male_Shank3B_plus_plus_small_intenstine
DNA was extracted and amplified using 16S rDNA bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP) using an Illumina MiSeq 20,000 sequence diversity assay (MRDNA, Shallowater, TX). The 16S rRNA gene V4 variable region PCR primers 515/806 with the barcode on the forward primer