GSM8809559: Female explanded blastocyst, rep2; Bos taurus; RNA-Seq
For RNA-seq samples, there was no separate extration step. For Iso-seq sample, RNA was extracted using PicoPure™ RNA isolation kit (KIT0204, Applied Biosytems™) with Dnase treatment (RNase-Free DNase Set, Qiagen). For RNA-seq, libraries were constructed using SMART-Seq® HT PLUS Kit (R400748, Taraka), and 15 PCR cycle were applied  for cDNA synthesis and 13 PCR cycles for library amplification. For Iso-seq, ISO-Seq® express 2.0 kit for cDNA synthesis, SMRTbell® prep kit 3.0 for library preparation.