PacBio raw HiFi seqences of Acropora pulchra
DNA was extracted by DNA Sequencing Center at Brigham Young University using the Qiagen Genomic Tip protocol and buffers (Qiagen Cat \# 10223). The samples were ethanol (2x) precipitated post column elution, put in the -20C freezer overnight and then spun for 30 minutes at 14K rcf the following day. Ethanol was removed and the DNA pellets were suspended in low TE buffer. The resulting DNA was cleaned prior to library prep with the PacBio SRE Kit (PacBio Cat \# 102-208-300) to remove fragments under 25kb. Following extraction, DNA was sheared to ~17kb using a Diagenode Megaruptor (Diagenode Cat \# B06010003) and checked on an Agilent Femto Pulse system (Agilent Part \# M5330AA) to assess size. The DNA was then cleaned and concentrated post-shearing using a 1x AMPure bead cleaning (AMPure Cat \#20805800). The DNA was then put into a library using the PacBio SMRTbell prep kit 3.0 (PacBio Cat \# 102-141-700), following the instructions provided with the kit. The final sizing of the library was performed using the 35\% v/v dilution of AMPure PB beads (AMPure Part \#  100-265-900). The single SMRTbell library was then sequenced using one 8M SMRT Revio Cell, and run for 29 hours on a PacBio Revio sequencer. Consensus accuracy circular consensus sequencing (CCS) processing was used to generate HiFi reads.