GSM8978537: Human knee synovial cells siUHRF1_rep2; Homo sapiens; RNA-Seq
Ctrl and UHRF1 KD cells were cultured in 6-well plates (1 x 10^5 cells/well) for 1 week Total RNA samples were extracted using RNeasy spin column kit and DNase I. Libraries were prepared by BRB-Seq(Alpern D, et al.) method. Individual samples were reverse transcribed using dT primer with unique cell barcodes. Then replicate were mixed and amplified by PCR. 10nt of UMI