Nanopore Sequencing of recombinant AAV producer plasmid
Libraries used PCR-free ONT Ligation Sequencing Kit (SQK-NBD114.24) with the NEBNext Companion Module (E7180L). GridION sequencer using R10.4.1 flow cells. Run design 400bps sequencing mode with a minimum read length of 200bp. Dorado (v0.9.1) super-accurate basecalling (SUP), demultiplexing, and adapter removal ('sup,5mC_5hmC'). No quality trimming has been performed.