ATAC-seq of human-chimp iPSC-derived tetraploid patterned induced neurons harvested at 2 hr post-KCl stimulation
20µl of the eluent was PCR amplified with 1X NEBNext High Fidelity PCR Master Mix (New England Biolabs, cat #M0541L) and primer sequences containing Illumina adapters for 72°C for 5min, 98°C for 30s, and then 5 cycles of 98°C for 10s, 63°C for 30s, and 72°C for 1min.  Libraries were purified using the DNA Clean and Concentrator-5 Kit and quantified with the KAPA Library Quantification Kit (Roche, cat #07960140001).