GSM9416247: 8025A; Mus musculus; RNA-Seq
24 hours post-Wnt inhibition organoids were lysed in Buffer RLT and 1% b-mercaptoethanol, and RNA was isolated using the Qiagen RNeasy kit. On-column DNase I digestion was performed according to the manufacturer's instructions. RNA libraries for RNAseq were prepared using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext UltraExpress RNA Library Prep Kit following manufacturer instructions