GSM9009135: STCO_1-3_S32; Mus musculus; RNA-Seq
Total RNA was extracted from sorted CD103+, CD49a+ and CD103negCD49aneg CD8 TIL populations with a single-cell RNA purification kit (Norgen Biotek) used in accordance with the manufacturer's instructions. RNA integrity (RNA Integrity Score≥7.0) was checked on an Agilent 2100 Bioanalyzer, and the amount of RNA was determined with Qubit (Invitrogen). The SureSelect Automated Strand-Specific RNA Library Preparation Kit was used, and the fragmented mRNA samples were used for cDNA synthesis. The cDNAs were converted into double-stranded (ds)DNA, and the final dsDNA libraries were subjected to paired-end sequencing on a HiSeq-2000 sequencer (Illumina),