GSM8844222: H9 embryonic stem cell rep2; Homo sapiens; ATAC-seq
Cells were lysed in an ice-cold ATAC lysis Buffer, nuclei were incubated in a tagmentation Master Mix for 30 minutes at 37°C, and the resulting DNA was purified using DNA purification columns. PCR amplification of the tagmented DNA was then carried out to generate libraries with appropriate indexed primers. DNA libraries were cleaned up with SPRI bead.