GSM8843612: Mouse liver, Normal diet 12 weeks, sample 2; Mus musculus; RNA-Seq
6-week-old male C57BL/6J mice were fed either a normal diet (ND) (n = 2) or a Western diet (WD) (n = 1) for 12 weeks, after which they were sacrificed. The liver was dissociated into single-cell suspensions using the pronase‒collagenase perfusion procedure. The collected cells were suspended in RPMI medium containing 2% FCS, filtered through a 70 μm mesh filter, and centrifuged at 500 × g for 10 minutes. After discarding the supernatant, the pellet was resuspended, and the process was repeated. The pellet was then resuspended in RPMI medium and centrifuged at 50 × g for 10 minutes to separate the supernatant, which contained non-parenchymal cells, from the pellet containing hepatocytes.  To enhance the analysis quality and focus on the function of non-parenchymal cells, the cells were mixed at an approximate ratio of 1:4 (hepatocytes to non-parenchymal cells). Single-cell analysis was performed using the BD Rhapsody™ Express System (BD Biosciences) according to the manufacturer's protocol. Single-cell analysis was performed using a BD Rhapsody™ Express System (BD Biosciences) according to the manufacturer's protocol. Rhapsody WTA libraries were generated and sequenced on a DNBSEQ-G400RS (MGIF-400; MGI Tech Co., Ltd., Shenzhen, China).