shotgun metagenomics of Rhinella marina; Puerto Rico, USA
Toad was hand-captured from the wild at night and sampled that night. Toad was rinsed with milli-q water before swabbing with a Copan flocked swab, using 30 strokes of the swab to sample the entire body, avoiding the face and cloaca. Sample was stored in 300uL Zymo DNA/RNA Shield, chilled in the field, and frozen in the lab at -20C until DNA extraction with Norgen Microbiome kit. Shotgun metagenomics sequencing was conducted at Ramaciotti Centre for Genomics.