GSM8978590: P023, Bulk CD4, Baseline, TCRb; Homo sapiens; OTHER
PMBCs were thawed in RPMI 20% human serum, centrifuged 7 min 200g. CD4+ T cells were sorted using magnetic column after labelling with CD4 MicroBeads (130- 096-553, Miltenyi Biotec). CD4+ T cells were lysed in 300µL Lysis/Binding Buffer for Dynabeads mRNA Purification Kits and stored at -80C (A33562, Invitrogen).