RNA-seq of pooled sample of Mastomys species
We used Glasgow Polyomics sequencing service for shotgun sequencing. Samples were prepared using the QIAseq FastSelect Epidemiology Kits (Qiagen) to remove the rRNA and then the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) for Illumina to create libraries. They sequenced samples on an Illumina NextSeq2000 using a P3 200-cycle cartridge, 2 x100bp with a target of 5M reads per sample.