16S rRNA gene sequencing of gastrointestinal samples from C57BL/6J
DNA was extracted from gastrointestinal samples using the ZymoBIOMICS Quick-DNA Fecal/Soil Microbe 96 Magbead Kit (colon samples) or QIagen DNEasy Blood and Tissue kit (stomach samples). We targeted the V3V4 region of the 16S rRNA with the 341F and 805R primers. Library preparation and sequencing was carried out at Novogene UK