GSM8775770: PRO, WT, 5 min recovery after heat-treatment for 5 min, rep1; Schizosaccharomyces pombe; OTHER
Cell permeabilization and extraction of biotinylated RNA for PRO-seq was performed as previously described (Mahat et al., 2016). cDNA libraries for PRO-seq were converted from the RNA, to which 3' and 5' adaptors were ligated, by revearse transcription.