PC9F3-ENU-X1 cell line WGS
DNA was extracted from 96-well plates using the AllPrep DNA/RNA Kit (Qiagen). DNA was amplified using the PCR+ TruSeq DNA Nano kit (Illumina) and IDT for Illumina TruSeq DNA UD Indexes plate. Quantification was performed using the Qubit 1X dsDNA High Sensitivity (HS) Assay (Invitrogen) and Kapa Library Quantification Kit (Roche) in order to optimize sample pooling. Pooled libraries were then prepared at 4 nM for a 2x151bp dual indexed sequencing run.