GSM9041059: TacHetNeg_P14_BL_10Hz_2h_Exp1_OP108; Mus musculus; RNA-Seq
Bulk RNA was extracted using the RNeasy Plus Universal Mini QIAGEN kit. Libraries were prepared using a modified SMART-Seq2 protocol. In brief, 1 ng of purified RNA was taken for reverse transcription with Maxima Reverse Transcriptase (Life Technologies) and whole-transcription amplification (WTA) with KAPA HotStart HIFI 2× ReadyMix (Kapa Biosystems) for 20 cycles. WTA products were purified with Ampure XP beads (Beckman Coulter), quantified with the Qubit dsDNA HS Assay Kit (ThermoFisher), and assessed with a high-sensitivity DNA chip (Agilent). RNA-seq libraries were constructed from purified WTA products using the Nextera XT DNA Library Preparation Kit (Illumina).