special rice-X571 (CRX1637862; CRA024990)
Genomic DNA from young leaves was extracted using a cetyltrimethylam-monium bromide (CTAB)-based protocol (Uzunova et al., 1995). The concentration and quality of the total genomic DNA were determined using a NanoDrop2000 Spectrophotometer (Thermo Fisher Scientific). DNA libraries(350bp) for Illumina/BGI sequencing were constructed for each accession according to the manufacturer's specifications. After DNA library construction, sequencing was performed on an Illumina HiSeq XTen platform by a commercial service (Biomarker Technologies, Beijing, China), with 150-bp read lengths. Raw reads were filtered based on the following criteria: pair-end reads with >10% 'N' bases; Reads, on which more than 50% of the bases have a quality score less than 38(Phred-like score); Sequencing adapter.Finally, high-quality sequences were obtained for subsequent analyses. submitted_instrument_model: Illumina HiSeq X Ten