Raw data of V3-V4 region of 16S bacterial gene
Sequencing libraries were generated, and indexes were added. The library was checked with Qubit dsDNA BR Assay Kit (Thermo Fisher Scientific, MA, United States) and real-time PCR for quantification and Bioanalyzer (Agilent, USA) for size distribution detection. Quantified libraries were pooled and sequenced on Illumina NovaSeq 6000 platform, according to effective library concentration and data amount required.