GSM9150226: Has2+/- KO mice 4days after elastase stimulation 5; Mus musculus; ssRNA-seq
Lung were removed, flash frozen on dry ice, and total RNA was extracted from mouse lungs using the TRIzol® reagent (Thermo Fisher Scientific) according to the manufacturer's instructions. RNA-seq libraries were prepared using a NEBNext rRNA Depletion Kit (New England Biolabs, Ipswich) and an ENBNext Ultra Directional RNA Library Prep Kit (New England Biolabs) according to the manufacturer's instructions using 500 ng of total RNA samples.