cytb metabarcoding of PCR grade water: Laboratory control sample
PCRgrade water serving as a library prep control was amplified with cytb metabarcoding primers (Snyder and Stepien 2020) targeting fsh species with Nextera stye overhangs, and dual indexed with Nextera indices following the Illumina 16S metagenomics protocol (Illumina Part# 15044223 Rev B). Samples were 250 bp paired-end sequenced on an Illumina MiSeq v3.