GSM8844398: APP-Female-3; Mus musculus; RNA-Seq
Total RNA was extracted using the RNeasy Mini kit (Qiagen, Germany) and integrity measured with an Agilent RNA Pico Bioanalyzer assay (Agilent Technologies). Libraries were generated using TruSeq standard mRNA kit (Illumina) according to manufactures instruction in with 200ng RNA input. Sequencing was performed at the Health Sciences Sequencing Core at UPMC Children's Hospital of Pittsburgh on NextSeq 2000 (Illumina).