GSM8978522: Sp5_8DKO_TLE_2; Mus musculus; ChIP-Seq
genomic DNA ChIP-seq samples were pooled and sequenced on NovaSeq Xplus 1.5B using TruSeq® ChIP SMP Prep and single-end sequencing. All the samples have percent of Q30 bases above 94%. All the samples have yields between 42 and 67 million pass filter reads. Samples were trimmed for adapters using Cutadapt before the alignment. The trimmed reads were aligned with mm10 reference using Bowtie2 alignment. Overall alignment percentage for the samples is 96%. Unique alignment is above 68%. There are 1.67 to 7.82% unmapped reads. Library complexity is measured by uniquely aligned reads using picard's markduplicate utility. All the samples have library complexity with percent non-duplicated reads ranging from 30 to 84%