RNA-seq of Stylosanthes guianensis (CRX1544993; CRA023524)
Total RNA was isolated from plant tissues using Trizol reagent, followed by precise quantification with the Qubit RNA HS Assay Kit. Poly(A)+ mRNA was subsequently enriched through oligo(dT)-coupled magnetic bead selection (mRNA Capture Beads), yielding high-integrity templates for library construction.