GSM8842708: Whole gonad E12.5 XY WT Rep3; Mus musculus; RNA-Seq
RNA was extracted using Qiagen RNeasy Plus micro kit (Qiagen, 74034). 140ng of total RNA was taken from each sample for library preparation. mRNA was transcribed to cDNA and prepared into libraries using Poly(A) mRNA Magnetic Isolation Module (NEB, E7490S), along with NEBNext UltraExpress RNA library prep kit (NEB, E3330S) and cleaned using AMPure beads (Beckman coulter, Bc-a63881) according to manufactures instruction. Unique indexes were added to each sample using ++NEBNext Multiplex Oligos for Illumina kit (NEB-E6440S) according to manufactures instruction and cleaned using AMPure beads.