GSM9010183: Vsx2 (Re3); Mus musculus; OTHER
The samples were processed following the CUT&Tag-It Assay Kit protocol (Active Motif, cat # 53170). Shortly, the cells from freshly dissociated r1 tissues were bound to concanavalin A coated magnetic beads followed by the antibody incubations and washing steps on beads. The following primary antibodies were used: rabbit anti-Gata2 (Abcam, ab109241), rabbit anti-Gata3 (Boster, M00593), rabbit anti-Tal1 (Abcam, ab75739), rabbit anti-Vsx2 (Proteintech, 25825-1-AP-20), rabbit anti-Ebf1 (Boller et al., 2016) rabbit anti-Insm1 (Nordic BioSite, ASJ-IO4DE3-50), rabbit anti-Tead2 (Biorbyt, orb382464), rabbit anti-IgG (Cell Signalling, 66362) and rabbit anti-H3K4me3 (Cell Signalling, 9751). The library  preparation was done as part of the CUT&Tag-It Assay Kit protocol (Active Motif, cat # 53170) that uses protein A-Tn5 transposomes (pA-Tn5) for tagmentation followed by DNA amplification with Q5 high-fidelity DNA polymerase and dual-barcoding primers. The number of PCR cycles was 14. The DNA libraries were multiplexed at 10 nM equimolar ratios and sequenced on NextSeq 500 or Aviti. 75 bp paired-end sequences were obtained.