GSM9271445: adipose stromal cells, passage 10, donor E; Homo sapiens; RNA-Seq
Total RNA was extracted from cryopreserved ASC pellets using the TRIzol reagent workflow according to the manufacturer's instructions. Poly(A)+ RNA was captured with oligo(dT) magnetic beads, fragmented, and converted to double-stranded cDNA; libraries were end-repaired, A-tailed, adapter-ligated, PCR-enriched, denatured to single-stranded circular DNA, QC-verified, and sequenced on the MGI DNBSEQ platform (PE100).