GSM9364587: shControl_zsGreen_Pool165_26; Mus musculus; RNA-Seq
Cells were FACS-sorted on an AriaIII (BD). Tumour cells were sorted as single cells/DAPI-/CD45-/tdTomato+. Niche cells were sorted as single cells/DAPI-/CD45+, then as CD3/CD8/CD49b+ (all 3 stained in same channel). Samples from different lungs were pooled after sorting for sequencing. Sorted cells were washed and resuspended in PBS+0.04% BSA (400 µg/ml) buffer at cell concentration of 1000 cells/µL. Libraries were prepared using the 3' v3.1 HT kit from 10x Genomics following manufacturer's instructions with 10,000 cell recovery and sequenced at 50,000 reads/cell.