GSM9248681: Monocyte after 4 days in co-culture, polarized with LPS/IFN-g; Homo sapiens; RNA-Seq
The sorted single-cell suspensions were loaded onto the Chromium Single-Cell Controller (10X Genomics) for droplet-based single-cell capture according to the manufacturer's protocol. Subsequent library preparation was performed according to the Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1 (10X Genomics, 14-16 cycles). Quality and quantity of the libraries was evaluated using the Bioanalyzer (Agilent) and Qubit (ThermoFisher), respectively. Sequencing was performed on an Illumina NovaSeq 6000 platform (paired-end, 150 bp reads length).