MinION sequencing
DNA from milk samples was extracted using the ZymoBIOMICS DNA 96 MagBead Kit, and libraries were prepared with the Rapid Barcoding Kit 24 V14 (SQK-RBK114.24) protocol from ONT on R10 flow cells (FLO-MIN114, ONT) using a MinION Mk1C device, followed by base-calling in HAC mode.
Rapid Barcoding Kit 24 V14 (SQK-RBK114.24)