GSM8777141: MCF-7, GFP, lncRNA1456 KD set1 Rep1; Homo sapiens; RNA-Seq
Total RNA was isolated from MCF-7 or MDA-MB-231 cells using the RNeasy kit (Qiagen, 74136) according to the manufacturer's instructions. For xenograft tumors, tissue was homogenized in RTL Plus Buffer (Qiagen 74136). PolyA+ RNA-seq libraries were prepared from control and lncRNA1456 knockdown MCF-7 cells as described above using the dUTP method.  Two biological replicates were generated for each sample.