GSM8667830: Xenograft D458 tumor No RT_160; Homo sapiens; RNA-Seq
D458 xenograft tumors were harvested from the cerebellum of mice. Tumors were chopped with razor blades and dissociated in RPMI media. Live cell suspensions were flow sorted based on green fluorescent protein (GFP) expression on a MoFlo XDP-Flex (Beckman Coulter) and 2,000/mL GFP+ cells were submitted to the University of Colorado Genomics core for scRNA sequencing with the Chromium X. N=6 10x Genomics Library preparation protocol