Metagenomics of Danio rerio: 5 dpf larvae
Sequencing was performed by BaseClear, Leiden using the Illumina NovaSeq platform at a sequencing depth of 2GB. Libraries were prepared using the Nextera XT Library Preparation Kit. FASTQ read sequence files were generated using bcl2fastq conversion software (v. 2.20; Illumina, San Diego, USA). Illumina Chastity filtering was applied, reads containing PhiX control signal were removed,  reads containing (partial) adapters were clipped up to a minimum read length of 50 bp. De novo metagenome assembly was performed based on contigs 1000 bp using MEGAHIT (v. 1.2.9).