Leaf Transcriptome of Canavalia ensiformis
Total RNA was isolated and mRNA was enriched using poly A selection module.The enriched mRNA was fragmented usingenzymatic methods to match the expected insert size. The fragmented DNA was ligated with Illumina-compatible Adapter.PCR was conducted to amplify the library and to generate the full-length adaptor containig unique index sequence in eachlibrary samples. The quality and quantity of library samples were determine using Tape Station, and qPCR. The librarysamples were proceeded to sequencing with 300 cycle (150-PE) using Illumina NextSeq 2000.