GSM8704845: 20201207-NMK52MU1; Mus musculus; OTHER
Mice at E16.5 and P0 were euthanized by decapitation. Older mice were euthanized by carbon dioxide asphyxiation. For each mouse, one kidney was embedded in optimal cutting temperature compound (OCT) and the other one was fixed in 10% neutral buffered formalin (Epredia, 5725) and then embedded in paraffin. For Visium: Fresh mouse kidneys were embedded with OCT on dry ice. OCT-embedded tissues sectioned at 10 μm thickness were then placed on Visium Spatial Gene Expression Slide following Visium Spatial Protocols-Tissue Preparation Guide (10X Genomics, CG000240 Rev A). Visium ST libraries were constructed following Visium Spatial Gene Expression Reagent Kits User Guide CG000239 Rev A (10X Genomics).