GSM8669286: untx_cb1d; Homo sapiens; RNA-Seq
The Promega Maxwell RSC simplyRNA cells kit including DNase treatment was used for RNA extractions using the Maxwell RSC instrument. A nanodrop was used to assess RNA concentration and the 260/280 ratio. RNA integrity was assessed using the Agilent TapeStation. Poly(A)+ selected reverse stranded RNA sequencing libraries were prepared from 4 control and 3 VCP lines using the KAPA mRNA HyperPrep Library kit for Illumina®, with 50 ng of total RNA as input. Libraries were sequenced on the NovaSeq 6000