GSM8647873: MCM, scRNAseq; Homo sapiens; OTHER
Tissues were obtained during assist device implantation and were formalin-fixed then paraffin-embedded (FFPE). RNA quality was performed on FFPE tissue sections (10 μm) using Total RNA Pico assay (Agilent technologies) which were then processed according to the 10X Genomics Visium CytAssist manufacturer's instructions. Briefly, sections were mounted on a glass slide under RNase-free conditions and libraries were prepared with Visium Human Transcriptome Probe Set v2.0. Sequencing was performed using DNBseq-G400RS (MGI Tech Co.). Raw FASTQ files and histological images were processed using 10X Genomics Space Ranger software (version 2.0.1). Alignment was performed using the GRch38-2020-A reference genome. libraries were prepared with 10X Genomics Visium Human Transcriptome Probe Set v2.0.