GSM8843232: DON_ACRYLAMIDE - Rep2; Rattus norvegicus; RNA-Seq
Cells were then collected in Extract-All (Eurobio Scientific) RNAseq libraries were constructed from 1000 ng of total RNA at the GeT‐TRiX facility (GénoToul, Génopole Toulouse Midi-Pyrénées) using Illumina® Stranded mRNA Prep kit (Illumina, San Diego, CA, USA) following the manufacturer's instructions. Briefly, mRNAs were captures, then fragmented, converted to double stranded cDNAs and sequencing adaptors were ligated. Libraries were amplified with ten cycles of PCR.