HvKP whole genome sequencing
Bacterial isolates were cultured on blood agar plates and harvested using a 10 L inoculating loop. Cells were lysed via mechanical disruption using MagMAX bead bashing tubes, and genomic DNA was extracted manually with the MagMAX DNA Multi-Sample Ultra 2.0 kit. Sequencing libraries were prepared manually following Illuminas DNA Prep protocol, utilizing bead-linked transposomes. Final libraries were sequenced on an Illumina iSeq100 platform.