GSM8616932: WT, B1-a cell,  2month, female; Mus musculus; RNA-Seq
5000 B-1a cell were isolated into lysis buffer,then  the process involves the following steps: Reverse transcription of RNA into cDNA; Amplify and purify the cDNA; two replicates of each sample were done. 5000 cells analysed per experiment. Quality control and counting of single cell suspensions, generally requiring a cell viability of 85% or more, washed and resuspended to a suitable cell concentration of 700~1200 cells/µl, ready for uploading onto the 10x Genomics Chromium™ system. Library was performed according to the manufacter's instructions(single cell 3' v2 protocol, 10x Genomics). The cDNA qualified by QC is subjected to second-generation sequencing library construction, and the library is finally subjected to quantitative QC after fragmentation, ligation of sequencing junctions, and sample Index PCR, and other experimental processes.