GSM8911341: KYSE30 Cells YTHDF3 (Input); Homo sapiens; RIP-Seq
Cells were lysed to isolate soluble RNA-protein complexes for subsequent immunoprecipitation RNA from IP and input samples was fragmented and reverse-transcribed into cDNA using random hexamer primers. Unique Molecular Identifiers (UMIs) were added during adapter ligation to enable deduplication. After end repair, adapter ligation, size selection, and PCR amplification, the libraries (~300 bp) were quality-checked and sequenced on the Illumina platform using paired-end 150 bp reads.