Single-cell RNA-sequencing of bone marrow-resident and circulating tumor cells from patients with Multiple myeloma and its precursor conditions
Libraries were prepared using the Chromium Next GEM Single Cell 5' Reagent Kit v2 (Dual Index), the Chromium Single Cell Human BCR Amplification Kit, and Library Construction Kits (10X Genomics), according to the manufacturer's instructions. Libraries were sequenced on a NovaSeq 6000 S4 flow cell at the Genomics Platform of the Broad Institute of MIT and Harvard (Cambridge, MA).