16S metagenome of Homo sapiens: Jejunum of adult male
Swabs of the GI tract taken during surgery were vortexed rigorously to ensure the release of bacteria from the swab into the DNA/RNA shield medium provided by ZymoResearch. Then, 750 microl of medium, containing the microbes, was used for mechanical lysis. Mechanical lysis was performed at 6 m/s for 45 s, repeated three times with 30 s intervals on ice between each lysis step. For profiling microbiome composition, the microbial DNA was sent to Novogene Company Limited (Cambridge, UK). To amplify the V3-V4 region of the 16S ribosomal RNA gene, polymerase chain reaction (PCR) with the following primers was used: CCTAYGGGRBGCASCAG, GGACTACNNGGGTATCTAAT.