GSM9013034: Donor1 CAIS; Homo sapiens; RNA-Seq
Fresh testes parenchyma was washed twice in PBS and subjected to enzymatic digestion (collagenase type I, collagenase type IV, hyaluronidase, trypsin, Deoxyribonuclease I; all enzymes at 1 mg/ml in DMEM-F12 without serum) for 10 minutes at 37°C with gentle agitation, repeated twice (1ml of digestion medium each time). The cell suspension was filtered through strainers with mesh size 70µm, washed twice with 0.4% BSA/PBS and filtered through a 40µm cell strainer to obtain single cell. Chromium Single Cell 3′ Library & Gel Bead Kit v2 kit (10xGenomics).