GSM9248307: CAB170-S2; Mus musculus; RNA-Seq
Fragments of frozen tumors were homogenized in TRIzol using a tissue homogenizer. RNA was extracted using the Invitrogen PureLink RNA Mini Kit according to the manufacturer's protocol. Libraries were prepared with the Ovation RNA-Seq Systems 1-16 (Nugen) using 100 ng of DNAse-treated RNA, and indexed libraries were multiplexed in a single flow cell