GSM8911960: RNAseq heterozygous c-Kit-humanized mouse-2 c-Kit-positive spermatogonia; Mus musculus; RNA-Seq
NucleoSpin RNA plus (TaKaRa) or RNeasy Fibrous Tissue Mini Kit (QIAGEN) for RNA. For mRNA-seq in spermatogonia of c-Kit-humanized mice, libraries were generated using a Hieff NGS Ultima Dual-mode mRNA Library Prep Kit for Illumina (Yeasen) and subjected to paired-end sequencing (150bp) with NovaSeq X plus (Illumina). For total RNA-seq in Apobec3-humanized BALB/c mice,　libraries were generated using an Illumina Stranded Total RNA Prep with Ribo-Zero Plus (NEB) and subjected to paired-end sequencing (100bp) with NovaSeq X plus (Illumina).