GSM8704971: TPC-1 cell line, sh-CHI3L1_KD2; Homo sapiens; RNA-Seq
Total RNA was extracted using NucleoSpin® RNA Plus (Takara Bio, Shiga, Japan) RNA libraries were prepared for sequencing using standard Illumina protocols. First use Qubit2.0 Fluorometer for preliminary quantification, dilute the library to 1.5ng/ul, and then use Agilent 2100 bioanalyzer to detect the insert size of the library. After the insert size meets expectations, qRT-PCR accurately quantifies the effective concentration of the library (the library is effective Concentration higher than 1.5nM) to ensure library quality After passing the library inspection, different libraries are pooled according to the requirements of effective concentration and target off-machine data volume, and then Illumina sequencing is performed.