GSM9012098: CD8+ T cells, WT1 (p38α+/+p38β+/+), biol rep 1; Mus musculus; RNA-Seq
RNA was extracted using RNAiso Plus (Takara). 1 μg of total RNA was used for the construction of sequencing libraries. mRNA was enriched from total RNA using Oligo(dT) magnetic bead, and then fragmented ramdonly. cDNA was synthesized from mRNA fragments using ramdon primers by M-MuLV reverse transcriptase. Thereafter RNA was degraded by RNaseH and the complementary DNA strand was synthesized by DNA polymerase I using dNTPs. After end repairing, polyA elongation and adding sequencing adaptor, around 370-420bp purified double strand cDNA was selected using AMPure XP beads. The cDNA was PCR amplified and further purified using AMPure XP beads to obtain the cDNA library.