GSM9149419: SH-SY5Y KI/KI RA treatment, Day7 replicate 2; Homo sapiens; RNA-Seq
Total RNA was extracted using ExtractRNA reagent (Evrogen), treated with DNase I (NEB) to remove genomic DNA, and RNA integrity verified via Qubit RNA IQ assay (RIN > 7.0). Poly(A)+ RNA was enriched using the NEBNext Poly(A) mRNA Magnetic Isolation Module, and strand-specific libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (average insert size ~283 bp).