GSM9285022: SNU16 cells, DCB_rep1; Homo sapiens; RNA-Seq
Total RNAs were subsequently isolated using the RNeasy Mini kit (Qiagen) following the manufacturer's manual. An in-column DNA digestion step was included to remove any residual genomic DNA contamination. To construct RNA-seq libraries, 500 ng total RNA was used for polyA mRNA-selection using NEBNext Poly(A) mRNA Magnetic Isolation Module (#E7490, New England Biolabs, Inc.  Ipswich, MA), followed by library construction via NEBNext Ultra RNA Library Prep Kit for Illumina (#E7530, New England Biolabs, Inc., Ipswich, MA).