HI-C_arima_4_enzyme_DNA
Two male and two female worms, whose specific identity were individually confirmed morphologically and molecularly, were pooled, weighed (3 mg) and then used for the construction of a library for subsequent Hi-C sequencing employing the Arima High coverage Hi-C kit (L/N2309050008; part no. A160162 v01; Arima Genomics, CA, USA). This library was constructed using cross-linked and proximally-ligated DNA, fragmented to 550660 bp using an ultra-sonicator (M220; Covaris, USA), then assessed for quality using the TapeStation (as described above) and paired-end sequenced in one lane of a Nova Seq X (10B, 300 cycles; Illumina, USA), with short reads being stored in the FASTQ format.