Bacterial 16S community from the biofilm of a settling plate on the Pensacola Edge Lander.
Total DNA from the biofilm of an aragonite settling plate was extracted using Qiagen PowerBiofilm kit. The V4 region of the 16S DNA gene were amplified using primers 515F/806R and sequenced on a MiSeqsystem with v2 chemistry to obtain paired-end 250-bp reads.