GSM9152035: Hippocampus, control male, rep2; Mus musculus; RNA-Seq
Total RNA from hippocampal brain tissue was extracted using the RNeasy Mini Kit (Qiagen) following manufacturer's instructions. After RNA extraction mRNA was enriched using poly-T oligo-attached magnetic beads. mRNA was then fragmented and reverse-transcribed into cDNA. After end repair, A-tailing, and adapter ligation, PCR amplification was performed to construct the final libraries. Libraries were sequenced on an Illumina platform.