GSM8956898: d614g_37C_rep2; Homo sapiens; RNA-Seq
For RNA extraction of primary epithelial cultures, reconstructed epithelia were infected for 2 days, then washed in cold PBS and then lyzed in TRIzol reagent (Thermo Fisher Scientific) added to the apical side of the insert. RNA was purified using the Direct-zol miniprep kit (ZR2080, Zymo Research), according to the manufacturer's specifications. RNAseq ibraries were prepared at BGI (Poland) for strand specific mRNA sequencing on a DNBSEQ platform with a PE100 sequencing read length.