Hepatic-Stellate-HHSC-A-RNA
RNA was isolated from sorted cells using the RNeasy Kit (Qiagen) according to the manufacturer's protocol and quantified by Qubit RNA BR assay (Thermo Fisher Scientific). Total RNA was validated using an Agilent RNA 6000 nano assay on the 2100 Bioanalyzer TapeStation  (Agilent Technologies). The resulting RNA Integrity number (RIN) of samples selected for RNAseq analysis was at least 7. RNA-sequencing libraries were prepared using TruSeq Total RNA library Prep Kit (Illumina) at Novogene Corporation Inc., and 150bp paired-end sequencing was performed on an Illumina HiSeq 4000 with a depth of 50 million reads per sample.