GSM9009068: Jurkat T cells, shsox4, RNA-seq, rep 3; Homo sapiens; RNA-Seq
Total RNAs were extracted from Jurkat T cells, hTERT-HMSMCs, human primary uterine myometrial cells, WISH cells or primary CD3+ T cells using TRIzol reagent (Invitrogen) following the manufacturer's protocol. The RNA integrity was assessed by Agilent 2100 Bioanalyzer system (Agilent Technologies, Santa Clara, CA, USA) and quantified by using NanoDrop 2000 (Thermo Fisher Scientific). RNA purification and reverse transcription for library construction were conducted by using VAHTS Universal V10 RNA-seq Library Prep Kit (Premixed Version), and sequencing were performed on an Illumina NovaSeq 6000 platform with paired-end 150 base pair reads, following the manufacturer's instructions (Illumina, San Diego, CA).