Metagenomic_sequencing_13 (CRX1580683; CRA024238)
For mNGS analysis, 1 microg of input DNA per sample was processed using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, USA). Briefly, DNA was fragmented to 350bp using a Covaris M220 focused-ultrasonicator (Covaris, USA). DNA fragments underwent end-repair, A-tailing, and adapter ligation followed by PCR amplification.