GSM9039999: vehicle: 5%glucose ip, PBS it, rep3, day7; Mus musculus; RNA-Seq
Tumor tissues were harvested and quickly suspended in an RNA extraction solution (ISOGEN). Total RNA was isolated according to the manufacturer's instructions. Total RNA (>1 ug) was used as input for mRNA enrichment using a sequencing kit (SMART-Seq v4 Ultra Low Input RNA Kit). RNA libraries for RNA-seq were prepared using TruSeq stranded mRNA Library Prep Kit following manufacturer's protocols.