The hybrid progenies-57 (CRX1735255; CRA025447)
Genomic DNA was extracted from the leaves using a DN14-Plant DNA Mini Kit (Aidlab, China). DNA purity was assessed via agarose gel electrophoresis. Subsequently, the DNA was fragmented by ultrasonic treatment, then subjected to terminal repair, 3'A-tailing, and ligation of sequencing adapters. Finally, PCR amplification was performed to construct the sequencing library. After quality inspection, the library was used for whole-genome re-sequencing on the HiSeq2500 system (Illumina, San Diego, CA, USA)