DNA_seq_of_homo_sapiens:gut_metagenome
DNA was extracted, quantified on a plate reader using buffer, dye, and standards from an Invitrogen Quant-iT(catalog number Q33120) or Qubit kit (catalog number Q32851), and normalized to 0.75 ng DNA/L. Tagmentation was performed using the Illumina Nextera DNA Library Prep Kit (catalog number 15028211) in a 2.5 mL reaction volume (Baym 2015). Tagmented DNA amplification was performed using KAPA HiFi HotStart ReadyMix (Roche, catalog number  07958935001). Amplified tagments were purified using AMPure XP beads  (Beckman Coulter  catalog number A63882). Purified libraries were pooled and sequenced