GSM8619037: WT_1; Mus musculus; RNA-Seq
RNA from FACS-sorted EC was isolated with an Arcturus PicoPure RNA Isolation Kit. The frozen pellet was resuspended in 50 μl of Arcturus PicoPure extraction buffer, and RNA was isolated according to the manufacturer's instructions. The RNA was eluted in 13 μl of RNase-free water, and the concentration was measured using a high-sensitivity RNA assay with a Qubit Fluorometer according to the manufacturer's instructions. A sequencing library was generated with 10 ng of total RNA using the SMARTer Ultra Low RNA Kit for Illumina Sequencing (Clontech) according to the manufacturer's protocol. Sequencing reads (100 bp paired-end reads) were generated on the HiSeq platform (Illumina) with four samples per lane.