GSM8977438: scRNA_MicePanc1_AndCellCulturePanc1; Homo sapiens; RNA-Seq
Cell suspensions were dispensed into the Takara ICELL8 nano-well chips. full-length SMART-Seq ICELL8 Reagent Kit was used to generate libraries according to the manufecturer protocol. In brief cDNA was synthesized, illumina libraries were generated with P5, P7 and Tn5 mixture. Following chip extraction and library pooling they were size selected using Agencourt AMPure XP magnetic beads (Beckman Coulter).