GSM8880870: Dorsal internal oblique muscles, IR-Atg200 (UAS-IR-FIP200), 3IL and APF of 1, 2, 3 and 4 day, rep1-4; Drosophila melanogaster; RNA-Seq
RNA was extracted using ReliaPrep Tissue RNA Miniprep System (Promega). Libraries were prepared by CEL-Seq2, a single-cell RNA-seq protocol (Hashimshony et al.) method. Individual samples were reverse transcribed using poly(T) primers with unique cell barcodes. Then replicate were mixed and amplified by PCR. 10nt of UMI followed by 9nt of cell barcode are in read1 and 81nt of RNA insert reads are in read2.