GSM8809380: M07e_C_Replicate_2; Homo sapiens; RNA-Seq
Genomic DNA was extracted from AML cell lines and primary cells using the AllPrep DNA/RNA Kits, Qiagen, following the manufacturer's instructions. DNA purity and concentration were assessed using a Qubit fluorometer. The integrity of the extracted DNA was evaluated using a bioanalyzer. mRNA library was prepared by (1) mRNA enrichment by poly (A) capture or rRNA removel, (2) RNA fragmentation, (3) cDNA reverse transcription, and (4) RNA library preparation.