RNA-seq of Arabidopsis: Wild-type replicate2
Twenty-five-day-old HYD1RNAi7, 10, 12 and Col-0 plants were harvested and ground into fine powder using liquid nitrogen. For each genotype, three independent biological replicates were prepared, with each replicate consisting of eight plants. Total RNA was isolated using RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) following the manufacturers protocol. Sample quality control, cDNA library preparation and sequencing were carried out by Novogene Corporation Inc. (Sacramento, California, USA).