GSM8778602: AD UD PSEN1 3; Homo sapiens; RNA-Seq
Total RNA was extracted using the SPLIT Total RNA Kit (Lexogen) following the manufacturer's protocol. RNA was quantified using a Nanodrop spectrophotometer, and RNA integrity was assessed using an Agilent 2100 Bioanalyzer. RNA samples with RIN > 7 were used for downstream library preparation. RNA-seq libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit (Lexogen) following the manufacturer's instructions. Approximately 40 ng of total RNA was used for library preparation, which included poly-A selection, cDNA synthesis, and library amplification using indexed primers.