GSM8647167: RD18_snATACseq_vehicle; Homo sapiens; ATAC-seq
Cells were processed according to the 10x Genomics Chromium Single Cell Multiome ATAC+ Gene Expression low cell number protocol conforming to protocol number CG000365_DemonstratedProtocol_NucleiIsolation_ATAC_GEX_Sequencing_RevB used for cell lysis (0.1x lysis buffer and lysed for 6.5 min) to obtain intact nuclei (10x Genomics, CA, USA). Single cell ATAC- and RNA-seq libraries were prepared using the Chromium Single Cell Multiome ATAC + Gene Expression Platform (utilizing the 10x Genomics Single Cell Multiome Kit) . Nuclei were prepared and counted to ensure quality and concentration. Nuclei were then transposed according to the manufacturers' protocol. Transposed nuclei suspension was loaded onto the Next GEM Chip J (Chromium Next GEM Chip J Single Cell Kit) targeting 5000 nuclei and then ran on a Chromium Controller instrument to generate GEM emulsion (10x Genomics). Single cell gene expression libraries, as well as single cell ATAC-seq libraries, were generated according to the manufacturers' protocol using the Chromium Next GEM Single Cell Multiome ATAC+ Gene Expression kit (10x Genomics). Final libraries were quantified using the NEBNext Library Quant Kit for Illumina (New England Biolabs, MA, USA) and High Sensitivity D1000 TapeStation (Agilent Technologies, CA, USA).