GSM9149316: Sublibrary 2, PlexinB1 Parse-seq; Mus musculus; RNA-Seq
To isolate nuclei from spinal cord tissue, approximately 10 mm of spinal cord encompassing the injury site was dissected and finely minced. Nuclei were extracted using a nuclei extraction buffer (Miltenyi Biotec, #130-128-024). The resulting suspension was enriched for nuclei using anti-Nucleus MicroBeads (Miltenyi Biotec, #130-132-997). Nuclei were subsequently fixed using the Evercode Nuclei Fixation Kit v3 (Parse Biosciences, ECFN3300). Split-seq cDNA libraries 49 were generated by iterative barcoding of nuclei via multiwell plating using the Evercode Whole Transcriptome (WT) v3 kit (ECWT3300; Parse Biosciences).