NGS of schistosoma japonicum
Extracted DNA was used to construct double-stranded DNA libraries following the protocol described in a previous study. Index primers were incorporated, followed by PCR amplification using Q5 High-Fidelity DNA Polymerase (NEB, USA) for up to 17 cycles. The resulting indexed libraries were purified with AMPure XP Beads (Beckman Coulter, USA) and subsequently sequenced on the Illumina platform(PE100).