RADseq of Lasius niger: 9991318
DNA was extracted from 3 legs of each sample. We used restriction enzymes EcoRI and Msel to digest the genomic DNA followed by ligating custom barcode adaptors to the DNA fragments, which were then amplified in 16 PCR cycles. Fragments with a size of 280 to 430 bp were selected using 2% agarose cassettes on a BluePippin (Sage Science). We sequenced these libraries on eight Illumina HiSeq 2500 lanes (single end, 150 bases) to generate the final read data.