GSM8618868: Group 3 innate lymphoid cell, WT NKp46+ ILC3s rep1, anti-IgG; Mus musculus; OTHER
Small intestinal ILC3s were sorted from siLP of aged  and young mice.  H3K4me3 Chip of three subsets of ILC3s were both performed on 100,000 cells  with modifications according to the protocol of Hyperactive In-Situ ChIP Library Prep Kit for Illumina (TD 901, Vazyme) Briefly, sorted CCR6+ ILC3s，NKp46+ ILC3s and DN ILC3s were washed with 500 μl of wash buffer containing 1× protease inhibitor cocktail (5056489001, Sigma-Aldrich). Cell pellets were resuspended in wash buffer. After two washes with binding buffer, Concanavalin A-coated magnetic beads were added and incubated at room temperature. Bead-bound cells were resuspended in 50 μl of antibody buffer.