RNA-seq of SCC152 cell line under normoxia
Cells were incubated under normoxic conditions for 24 h, placed into the RNAlater Solution (Thermo Fisher Scientific, Waltham, MA, USA), and stored at 4C for a maximum of 14 days. Samples were disrupted by a rotor-stator homogenizer (Omni TH, Kennesaw, GA, USA) in a lysis buffer, and total RNA was isolated using the NucleoSpin RNA kit (Macherey Nagel, Dren, Germany). NGS library was prepared using the QuantSeq 3mRNA-Seq Library Prep Kit (FWD) (Lexogen, Vienna, Austria). Sequencing was performed on the NextSeq 500 System (Illumina, San Diego, CA, USA), yielding unpaired 75-bp reads.