GSM8908501: RNAseq_IMR5_siTCEAL1_rep2; Homo sapiens; RNA-Seq
RNA was extracted using the Qiawave Kit (Qiagen) according to the manufacturer's instructions. RNA concentration and RQN values were determined with a Fragment Analyzer (Agilent) and 500 ng RNA with RQN > 9 was used for library preparation using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina following the manufacturer's manual. Libraries were size selected using SPRIselect Beads (Beckman Coulter) after amplification with 9 PCR cycles. Library quantification and size determination were performed with the Fragment Analyzer (Agilent) using the NGS Fragment High Sensitivity Analysis Kit (1-6,000 bp; Agilent). Libraries were subjected to cluster generation and base calling for 100 cycles paired end on the Illumina NextSeq 2000 platform.