GSM9009753: Nipponbare leaves, low phosphate, biol rep 2, L2; Oryza sativa; RNA-Seq
Total RNA was extracted from rice leaves of 21-day old plants that had been treated with different Pi supplies for 14 days using Maxwell RSC Plant RNA Kit (Promega) 200ng of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using Hieff NGS Ultima Dual-mode mRNA Library Prep Kit for Illumina (Yeasen) following manufacturer's protocols. For poly(A) mRNA purification, HieffNGS DNA selection Beads (magnetic pearls from Yeasen) were used.