GSM9041600: HFF Native DFF-ChIP Exp5 H3.3; Homo sapiens; ChIP-Seq
Rapid nuclei isolation steps were performed on wet ice using ice-cold buffers as previously described (M. Li et al. 2020). Following DFF digestion, nuclei were lightly sonicated for 20s and the soluble material was immunoprecipitated with antibodies. Library construction is detailed in the manuscript.