GSM8844435: Before_2; Homo sapiens; RNA-Seq
TRIzol was added to the samples and they were homogenized by IKA® Ultra-Turrax® tissue homogenizer (Sigma-Aldrich, St. Louis, MO, US) for the complete RNA isolation. According to the manufacturer´s protocol, 0.2 ml of chloroform added directly to 1 ml of TRIzol was used for lysis and separation of the aqueous phase containing RNA. 0.5 ml of isopropanol was mixed to precipitate RNA. The RNA pellet was washed with 75% ethanol and resuspended in 15 µl of RNase-free water before the quantity and quality of the RNA were assessed. The RNA library preparation and transcriptome sequencing processes were outsourced to Novogene Co., LTD. (Milton, United Kingdom), utilizing their Illumina platform for paired-end sequencing.