GSM9247960: KG1a large EV enriched glycoRNA, rep1; Homo sapiens; ncRNA-Seq
Total RNA and enriched glycoRNAs were both extracted using RNAzol combined with Zymo column purification Library preparation is performed using the MGIEasy Small RNA Library Prep Kit (BGI-Shenzhen, China). A certain amount of RNA is ligated 3' and 5' adaptors sequentially in two reaction systems for adaptor ligation. After adaptor ligation, RNA is reverse transcribed into cDNA and PCR amplified. The library is fragment size selected by polyacrylamide gel electrophoresis and subjected to quality control.