GSM9013232: KO_CD45_S1, scRNA; Mus musculus; RNA-Seq
Cells in  were fixed by 0.9 % formaldehyde (Sigma) for 10 min at room temperature followed by quenching with 125 mM glycine for 5 min and washed three times with PBS. Extracted nuclei were resuspended in ChIP dilution buffer and sonicated for 5 min using 4s on 11s off at 5% power using SCIENTZ-IID (Scientz Biotechnology) for ChIP-seq. RNA was extracted by Trizol for RNA-seq. Library was constructed using DNA or RNA library construction kit (Vazyme).