GSM9005937: ST-FF07172093; Homo sapiens; RNA-Seq
Tissue samples were extracted either by conventional methods or using the NICO Myriad system, which preserves RNA integrity through in situ collection in a refrigerated chamber with Ringer's Lactate. Total RNA was isolated using the Trizol based protocol following the manufacturer's protocol. circRNA libraries were constructed following ribosomal RNA depletion and enrichment for circular RNAs, using a strand-specific protocol compatible with Illumina sequencing platforms.