GSM9277885: RNA, NC 2; Homo sapiens; RNA-Seq
Total RNA was extracted from cells using TRIzol reagent (Invitrogen) according to the manufacturer's instructions. RNA integrity was assessed with the Agilent 2100 Bioanalyzer, and only samples with RIN ≥ 7 were used for library preparation. RNA-seq libraries were prepared using a 3′ mRNA-seq library preparation kit (e.g., QuantSeq 3′ mRNA-Seq Library Prep Kit for Illumina, Lexogen) according to the manufacturer's instructions. Briefly, libraries were generated from poly(A)+ RNA using oligo(dT) priming, followed by reverse transcription and second-strand cDNA synthesis from the 3′ end of transcripts.