GSM8667614: 458IR H3K27me3 GSK321_1; Homo sapiens; OTHER
5x105 cells were collected for analysis by CUT&RUN EpiCypher, CUTANA, CUT&RUN Kit Version 4 #14-1048) . Briefly, cells washed in wash buffer,  and resuspended in wah buffer plus activated beads.  The cells absorb the beads and are centrifuged and resuspended in antibody buffer. Antibody binds overnight at 4 degrees. Antibodies against, a-H3K4me3 and IgG controls were validated antibodies from EpiCypher. a-H3K27me3 (Cell Signaling technologies, #9733) and a-H3K27ac (Cell signaling technologies, #8173). CUT&RUN library prep was performed with the NEBNextâUltraII DNA Library Prep Kit for Illuminaâ (NEB #E7645L) and NEBNext Multiplex Oligos for Illumina (NEB#E7350).