MRA-Pantoea-FN0307 (CRX1643829; CRA025131)
Genomic DNA was extracted with the SDS method. The harvested DNA was detected by the agarose gel electrophoresis and quantified by Qubit2.0 Fluorometer (Thermo Scientific).A total amount of 1ug DNA per sample was used as input material for the DNA sample preparations. Sequencing libraries were generated using NEBNext UltraDNA Library Prep Kit for Illumina (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample. Briefly, the DNA sample was fragmented by sonication to a size of 350bp, then DNA fragments were end-polished, Atailed, and ligated with the full-length adaptor for Illumina sequencing with further PCR amplification.