I002B Duplex
Frozen cell pellets from lymphoblastoid cell lines were processed for HMW gDNA extraction using the Monarch kit, with shaking excluded to preserve gDNA integrity, then assessed for quality and prepared for duplex sequencing by shearing to a target size, performing size-selective precipitation, and constructing libraries for sequencing on the PromethION 24 with R10.4.1 flowcells.