WGS of Brassica napus
Long-read DNA sequencing using Oxford Nanopore Technology was conducted. DNA fragments smaller than 25 kbp were progressively depleted before library preparation using the Short Read Eliminator kit (Circulomics Inc., USA) to enhance read length and sequencing output. Library preparation was performed using the native ligation sequencing kit (SQK-LSK109; Oxford Nanopore Technologies), followed by sequencing using Oxford Nanopore Technology PromethION R9.4.1 flow cells.