Metagenomic sequencing results of viral communities from raccoon dog  lung tissue
Ten lung tissue samples were randomly pooled into three groups (designated Libraries 1, 2, and 3). The pooled lung tissues were subjected to purification and concentration of virus-like particles (VLPs) through sequential processing, including homogenization, filtration, and ultracentrifugation. Viral nucleic acids were coextracted from VLPs via combined DNA/RNA isolation protocols. Whole-genome amplification and deep sequencing of the viral strains were performed via Illumina sequencing technology (Shanghai Tanpu Biotechnology Co., Ltd.). The postamplification steps included DNase treatment, cleanup, second-strand synthesis, and library preparation with Nextera XT reagents, followed by sequencing on the NovaSeq PE150 platform (Illumina). The raw reads underwent quality trimming via Skewer, with additional filtering of unreliable sequences on the basis of user-specified Phred quality scores (Q20).