GSM9364902: splenic B220+ (Miz-1DPOZ) replicate 1; Mus musculus; RNA-Seq
For RNA isolation, B220+ splenocytes were lysed in TRI Reagent (Sigma) and isolated RNA was cleaned using the RNeasy Mini Kit (Qiagen). For RNA sequencing (RNA-seq), a biological duplicate of sequencing libraries was prepared from RNA extracts. Ribosomal RNA were depleted (Ribo-Zero™ Magnetic Gold Kit, Epicentre) and library was prepared using KAPA Stranded RNA-Seq Kit with RiboErase. NGS sequencing (pair-end 50 cycles) was done on HiSeq 2000/2500 System (Illumina).