GSM8843779: Human primary chodroctes treated with cyproheptadine 2; Homo sapiens; RNA-Seq
Total RNA was isolated with Direct-zol RNA MicroPrep kit (Zymo Research). Libraries for bulk RNAseq were prepared using an early access RNAseq library prep kit from Jumpcode Genomics. Basically, 20ng total RNA from each sample was reverse transcribed using random primers with partial P7 Illumina adapter sequence on the 5' end. After RT, a partial P5 adapter sequence was ligated to the 3' end of the cDNA molecule followed by PCR amplification with full length indexed Illumina adapter primers. PCR amplified library was treated with Jumpcode's CRISPRclean reagent to cut library molecules containing rRNA sequences.