GSM8881094: iCARUb IL12; Mus musculus; OTHER
Tumors (n=8 per cohort) were harvested at the study endpoint, minced, treated with collagenase, and filtered through a 70 μm cell strainer in PBS to generate single-cell suspensions. Live and dead cells from each tumor were counted, and aliquots containing approximately 50,000 live cells per tumor were prepared. Aliquots from mice within the same cohort were pooled together, and live cells were subsequently isolated using a live/dead cell separation kit. The pooled cell samples were centrifuged, resuspended in 300 µl of lysis buffer (catalog number: A33562), and stored at –80°C. Bulk TCR sequencing analysis was performed as described previously (Cell Reports Methods 2023, Genolet et al)