Off target HTS of GFPpm mouse heart
Genomic DNA was isolated with the NucleoSpin Tissue kit. 5-50ng was used as input for 30 cycles of PCR1 to amplify the genomic locus. 1uL of PCR1 was used as input for 10 cycles of PCR2 to append Illumina barcodes. NEBNext polymerase was used. Then library was pooled, gel extracted, normalized, and sequenced.