GSM8979218: RIB40, Control, 5h, rep2; Aspergillus oryzae; RNA-Seq
Total RNA was extracted from mycelia using TRIzol (Thermo Fisher Scientific) according to manufacturer's instructions with small modifications. Mycelium samples were ground using cooled mortars under liquid nitrogen into fine powder and about 50 µL of powder was transferred into a 2 mL tube, after which 1 mL of TRIzol was immediately added. Mixture was vortexed and incubated at room temperature for 5 minutes, after which it was centrifuged (12000 x g, 4 °C, 10 minutes) and the supernatant was transferred to a new tube. After this, the protocol was continued according to manufacturer's instructions. RNA was then purified using RNeasy Mini kit (Qiagen) according to manufacturer's instructions. After purification samples were sent for RNA sequencing, that was performed by CeGaT GmbH (Tübingen, Germany) using the Illumina NovaSeq 6000 platform (Illumina Inc., San Diego, CA). Library was prepared with TruSeq Stranded mRNA (Illumina) kit.