Whole genome re-sequencing of Chromochloris zofingiensis: mut. strain G9
Genomic DNA was extracted from tissue samples using the Universal Genomic DNA Extraction Kit (Magnetic Bead Method, China Maggi Yuhua Kit). The quality of the isolated DNA was assessed by two complementary approaches: (1) monitoring DNA degradation and contamination on 1% agarose gels, and (2) measuring DNA concentration with a ND-2000 spectrophotometer (NanoDrop Technologies). Only samples exhibiting high purity (OD260/280 = 1.72.1, OD260/230  2.0) were selected for library construction. Qualified genomic DNA was randomly fragmented by ultrasonication, followed by end repair, 3 end adenylation, and adapter ligation. Fragments of approximately 350 bp were enriched using magnetic beads and subsequently amplified by PCR to construct sequencing libraries. Libraries that passed quality control were sequenced on the Illumina NovaSeq X Plus platform using the PE150 strategy, generating paired-end reads with a total length of 300 bp.