Pacbio-HIFI-whole_genome_DNA
Genomic DNA (6.25 ng) from a single male was subjected to whole genome amplification using the REPLI-g midi kit (Qiagen, Germany), and the quantity and quality of this amplified DNA assessed in TapeStation system (Agilent 4200) using Genomic DNA ScreenTape (Thermo Fisher Scientific). One aliquot of this DNA (20 mg) was used to construct a PacBio Revio/SMRT library, which was sequenced in the PacBio Revio Platform (at BGI, Hong Kong), with sequence data stored in the FASTQ format.