16S sequencing of Drosophila melanogaster gut microbiome  Day 10, Parkin uninduced
This study aimed to investigate the impact of Parkin gene overexpression on the gut microbiome of Drosophila melanogaster across different ages. Adult flies overexpressing Parkin (ParkinOE) and age-matched wild-type (WT) controls were collected at four time points: Day 10 (young), Day 30 and Day 45 (middle-aged), and Day 60 (aged). For each condition, gut tissues from 100 individual flies were dissected under sterile conditions and pooled to obtain sufficient microbial DNA. DNA was extracted from each pooled sample and subjected to 16S rRNA gene sequencing. The experimental design allows for the comparison of gut microbiome composition between genotypes and age groups, to assess whether Parkin overexpression influences microbial dynamics related to aging and host physiology.