GSM9010054: CX-5461 exp3; Homo sapiens; RNA-Seq
Total RNA was extracted using “Total RNA Mini kit” (Geneaid Biotech, New Taipei City, Taiwan), using Ambion™ DNase I (RNase-free), according to manufacturer's instructions. The concentration and quality of the extracted total RNA were determined by measuring light absorbance at 260 nm (A260) and the ratio of A260/A280 (Qbit System - ThermoFisher). Sequencing libraries were generated using Nextflex Rapid Directional RNA-Seq kit 2.0 Kit with Poly(A) Beads 2.0 (PerkinElmer). Libraries were sequenced on Illumina NovaSeq with 2x150 bp paired-end run. Average seq throughput for the project ≥ 30M PE read per sample; Q30 ≥ 85%.