GSM9415232: MCC, SSRP1-FKBP-E14, MNase 8U, DMSO, clone13, tech rep 2; Mus musculus; OTHER
40M cells were fixed with 2% FA and permeabilized with 1% digitoning. Then, the chromatin was digested with MNase and ligated. After RNase and Prot K treatment, the DNA was purified with phenol-chloroform extraction and sonicated aiming for 200bp fragments. Sequencing libraries were prepared with NEBNext Ultra II (NEB 7645S). Regions of interest were captured with biotinylated probes using the HyperCapture Target Enrichment Kit (Roche 9075828001)