NGS profiling of sediment metagenome
DNA was isolated from the sediment samples using the FastDNA Spin Kit for Soil (MP Biomedicals, United States) and TissueLyser LT (QIAGEN, Germany) according to the manufacturers protocols. Preparation of amplicon libraries for the V3-V4 regions of the 16S rRNA genes, their sequencing and analysis were carried out as described previously (Khomyakova et al., 2022). For each sample, analysis was performed in at least two replicates.