RNASeq of felis catus: left atrium
Extracted tissues were immediately flash frozen in liquid nitrogen, and stored at -80C. Mature mRNA was selected using the polyA method. Samples with an RNA Integrity Number (RIN) of >7 were converted to cDNA. Converted samples were then processed for stranded library prep. Paired-end 150 bp cDNA libraries then underwent sequencing on the Illumina HiSeq platform.