WGS of Zephronia sp
Genomic DNA randomly fragmented by sonication, DNA fragments end polished, A-tailed, ligated with the full-length Illumina adapters, followed by PCR amplification with P5 and indexed P7 oligos. PCR product libraries purified with AMPure XP system. Libraries then checked for size distribution by Agilent 2100 Bioanalyzer (Agilent Technologies, CA, USA), and quantified by real-time PCR (to meet 3 nM).