Iso-Seq of Zi Goose
After the RNA (or polyA+ RNA) passes the inspection, the SMARTer PCR cDNA Synthesis Kit is used to synthesize the first - strand cDNA. Once the SMARTScribe Reverse Transcriptase is added to the 5' end of the mRNA, the terminal - repair transferase will add several additional base sequences to the 3' end of the cDNA. These bases serve as binding sites for oligonucleotide synthesis, extending the first strand of cDNA to the end of the oligonucleotide, generating a universal primer sequence that facilitates the synthesis of the second - strand cDNA and PCR amplification. Then, PCR amplification is carried out. After the library passes the inspection, according to the effective concentration of the library and the data output requirements, the PacBio Sequel IIe platform is used for sequencing.