RNA-Seq of L.decemlineata: 4st instar larvae haemocytes treatment M.robertsii
Total RNA was extracted from insect hemocytes and fat bodies using the Aurum Total RNA Mini Kit (Bio-Rad, 7326820). RNA concentration was measured with the Qubit RNA HS Assay (Invitrogen, Q32852). All samples had high quality (RIN  8.0) and sufficient yield (1 g). Libraries were prepared following the NuGEN protocol (Tecan), and fragment size and concentration were assessed using an Agilent High-Sensitivity chip. Sequencing was performed on the DNBSEQ-platform (sequencer DNBseq G400) at the Beijing Genomics Institute.