RNAseq of preflexion Vinciguerria mabahiss retina: sample DSK6E1
RNA extractions were shipped on dry ice and whole-retina transcriptome libraries were prepared from total RNA at Novogenes sequencing facilities in Hong Kong and Singapore. The Clontech SMART-Seq v4 Ultra Low Input RNA Kit and the NEBNext Ultra RNA Library Prep Kit for Illumina were used for larval samples. The NEBNext Ultra RNA library preparation kit for Illumina was used for adult samples. The concentration of each library was checked using a Qubit dsDNA BR Assay kit prior to barcoding and pooling at equimolar ratios. Libraries were sequenced as 150 bp paired-end reads on an Illumina NovaSeq 6000 S4 flow cell.