GSM9248948: WT control; Mus musculus; RNA-Seq
On day 4, 5 BRAFi+MEKi treated tumors and 2 control treated tumors were harvested, homogenized, and pooled into individual samples. Live cells were enriched using the Miltenyi Biotech dead cell removal kit followed by sorting the Annexin V negative population. Cell suspensions were adjusted to 1000 cells/μL and loaded onto a Chromium Controller (10x Genomics). scRNA-seq libraries were prepared using the Chromium Single Cell 3' & Gel Bead kit v3.1 (10x Genomics) according to the manufacturer's instructions. Reverse transcription and amplification were performed on a C1000 Touch Thermal Cycler (BioRad). The quality of cDNA and the final libraries were checked using the 4200 TapeStation System (Agilent). Libraries were pooled and sequenced at 400pM as the final loading concentration using the Illumina NovaSeq 6000.