GSM8907767: UD HF2; Homo sapiens; RNA-Seq
RNA was extracted using the RNeasy Mini Kit (Qiagen) according to manufacturer's instructions Libraries were generated from 250 ng of total RNA as following: mRNA enrichment and library preparation were performed using the Illumina Stranded mRNA Prep Kit (Illumina), as per the manufacturer's recommendations. Libraries were quantified using the KAPA Library Quantification Kits - Complete kit (Universal) (Kapa Biosystems). Average size fragment was determined using a Fragment Analyzer (Agilent) instrument.