PacBio sequencing of Staphylococcus epidermidis: LTH 7178.
1. DNA is fragmented by g-TUBE. 2. Fragmented DNA is processed for nick repairing. 3. Fragmented DNA is further processed for end repairing. 4. SMRTbell adapter is ligated to the prepared DNA fragments. 5. The ligation products are then treated by exonuclease to degrade failed ones. 6. Fragments with designed size were selected by BluePippin and ready for sequence.