WGS of Candida glabrata
The library construction method for Illumina sequencing using the TruSeq DNA PCR-Free Prep Kit involves fragmenting DNA by ultrasonication, repairing ends, and adding A-tails to prevent self-ligation. Adapters with index sequences are then ligated to the DNA fragments, followed by purification using AMPure XP beads to remove contaminants. Finally, the library is size-selected using agarose gel electrophoresis to achieve the desired fragment length, typically around 450 bp.