DNA-seq of microglia, neurons, glia and blood from AD and Controls
After PicoGreen quantification, ~200ng of genomic DNA were used for library construction using the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) with 8 cycles of PCR. After sample barcoding, 2.5ng-1µg of each library were pooled and captured by hybridization with baits specific to either the HemePACT (Integrated Mutation Profiling of Actionable Cancer Targets related to Hematological Malignancies) assay, designed to capture all protein-coding exons and select introns of 576 (2.88Mb) commonly implicated oncogenes, tumor suppressor genes and or HemeBrainPACT