WGS for Bf12P1 siphovirus, propagated in Bacteroides fragilis NCTC 9343 strain
Bf12P1 phage was amplified overnight in Bacteroides fragilis NCTC 9343 liquid culture started in Mega media.  The obtained phage lysate was filtered using a 0.22 m syringe filter to remove bacterial cells. An aliquot of the lysate was used for DNA extraction and sequencing. Phage DNA was extracted using the Norgen Phage DNA Isolation kit. Phage DNA was sequenced by the Microbial Genome Sequencing Center (MiGS; Pittsburgh, PA) using Illumina NextSeq 2000.