GSM8881925: Single-nucleus RNA-seq of cortical organoid from wild-type iPSC line at 2 months; Homo sapiens; RNA-Seq
Nuclei were isolated from individual organoids using the 10x Genomics demonstrated protocol for single-nucleus RNA-seq. Organoids were gently dissociated in lysis buffer to release nuclei, which were filtered through a 40 µm strainer, pelleted by centrifugation, and resuspended in nuclei buffer. Single-nucleus libraries were prepared using the 10x Genomics Chromium Single Cell 3' v3.1 kit according to the manufacturer's protocol. Approximately 8,000–10,000 nuclei per sample were loaded onto each Chromium chip channel. Libraries were sequenced on an Illumina NovaSeq 6000.