GSM9041522: In-vivo Bulk RNAseq Day21 Dabrafenib/Trametinib treated Mouse139; Homo sapiens; RNA-Seq
Post-treatment WM4237-1 cells were trypsinized, washed, and processed with Dead Cell Removal Kit (Miltenyi Biotec) following manufacturer's instructions. Tumor chunks from replicates were harvested and snap-frozen for bulk RNA-seq. Samples were lysed and homogenized in TRIzol reagent to release RNA. Libraries for whole transcriptome RNA sequencing were prepared using the Stranded Total RNAseq with Ribo-zero Plus kit (Illumina, San Diego, CA) as per manufacturer's instructions starting with an input of 700 ng of total RNA and 10 cycles of final PCR amplification. Library size was assessed using the 4200 Tapestation and the High-Sensitivity DNA assay (Agilent, Santa Clara, CA). Concentration was determined using the Qubit Fluorometer 2.0 (Thermofisher, Waltham, MA). Next Generation Sequencing with a paired-end 2x150bp run length was done on the NovaSeq X platform (Illumina, San Diego, CA). A minimum of 30 M reads per sample was acquired for each sample.