QuantSeq of immortalised bone marrow derived macrophages following replacement of culture media with fresh or conditioned media
QuantSeq of immortalised bone marrow derived macrophages following replacement of culture media with fresh or conditioned media
Immortalised bone marrow derived macrophages were cultured overnight in DMEM supplemented with 10% fetal bovine serum. Culture supernatant was removed and replaced with either fresh media or conditioned media (i.e. the original culture supernatant). RNA was extracted from cultured cells by Trizol purification according to manufacturer's instructions Libraries were generated with the Lexogen QuantSeq 3' mRNA-Seq Library Prep Kit FWD according to manufacturer's instructions