GSM9092285: HEK293T-EF1α-mCherry, tRNAAlaGGC overexpressed(tA), 3; Homo sapiens; RNA-Seq
Total RNA was extracted using Trizol (Invitrogen, Carlsbad, CA, USA), and subjected to mRNA isolation using GenSeq® mRNA Purification Kit (GenSeq, Inc.). The purified mRNA was used for library construction with GenSeq® Directional RNA Library Prep Kit (GenSeq, Inc.) according to the manufacturer's recommendations.