Mock_1
First, RNA is extracted from the biological material of cells. Second, subsets of RNA molecules are isolated using the poly-A selection protocol to enrich for polyadenylated transcripts. Next, the RNA is converted to complementary DNA (cDNA) by reverse transcription and sequencing adaptors are ligated to the ends of the cDNA fragments. Following amplification by PCR, the RNA-Seq library is ready for sequencing.