GSM8703937: OLIG2; Homo sapiens; ChIP-Seq
Cells were disassociated with Accutase to single cell. CUT-TAG (cleavage under targets & release using nuclease) chromatin profiling were performed using Hyperactive® Universal CUT&Tag Assay Kit (vazyme, TD903).] Antibody against Flag was used at 1:100 for 100,000 cells. DNA was collected for paired-end sequencing. The chromatin immunoprecipitation (ChIP) protocol for OLIG2 was adapted as below. Briefly, cells were crosslinked with 1% formaldehyde for 10 min at room temperature and then treated with 0.2 M glycine to inactive the formaldehyde. Cells were then lysed to obtain chromatin extracts, which were sonicated to obtain DNA fragments with an average size of 300-500 bp. The resulting chromatin extracts were immunoprecipitated by OLIG2 antibody (Sigama, AB9610) immobilized on Protein-G beads.