GSM9037233: maxillae bone, adult, control, rep1; Mus musculus; RNA-Seq
TRIzol reagent (catalog number 10296010, Invitrogen) was utilized for RNA extraction from each sample in accordance with the manufacturer' s instructions. The RNA purity was evaluated using a NanoPhotometer® spectrophotometer (IMPLEN) by measuring the absorbance ratio at 260/280nm and 260/230nm with acceptable values being greater than 1.8 and 2.0, respectively. RNA integrity was determined using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies), selecting samples with an RNA integrity number (RIN) exceeding 7 for subsequent library construction. RNA sequencing libraries were generated using the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB), following the manufacturer' s guidelines.