GSM9012163: Es-7-32; Eriocheir sinensis; RNA-Seq
Total RNA was first extracted from E. sinensis eyestalk samples across all temperature groups using the Trizol method, with extracted RNA stored in a -80°C ultra-low temperature freezer. A portion of RNA from each temperature group was pooled and subjected to third-generation transcriptome sequencing on the PacBio Sequel platform to generate a reference genome.