GSM8778068: PB1 knockout Schwann cells, replicate 1; Mus musculus; RNA-Seq
Total RNA from WT and PB1KO SCs was isolated using an RNeasy Micro kit (Qiagen) following instructions for on-column DNase digestion. RNA quality and concentrations were determined using an Agilent Bioanalyzer (Agilent RNA 6000 Pico, 5067-1513). For library construction using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina), the protocol follows the TruSeq Stranded mRNA Sample Preparation Guide, Part #15031047 Rev. E. This procedure begins with RNA isolation, followed by mRNA enrichment using poly(A) selection. The mRNA is then fragmented, reverse transcribed into cDNA, and adapter-ligated. After amplification, the library is purified, and its quality and quantity are assessed before sequencing.