GSM9361251: Tumour, 100 mg/kg STC-15, 12 h, rep3; Mus musculus; RNA-Seq
For RNA-seq, blood and tumour samples for were collected at termination. Up to 500 μL of blood was collected by cardiac puncture, stored in RNAprotect tubes (Qiagen, 76554) and left at RT. Tumours were cut in half, placed in cryovials and snap frozen. RNA from in vivo whole blood and tumour samples were generated into RNA-seq libraries by Novogene using their rRNA-depletion strand-specific sequencing service. Blood samples were also depleted for Globin RNA. Libraries were sequenced to a depth of greater than 40 million PE150 reads on a NovaSeq X plus Illumina machine. Data from five biological replicates were collected for each in vivo condition.