16S rRNA seq of Sus scrofa: Respiratory Tract
This study investigates the airway microbiome of swine following thermal injury and endotracheal tube placement. DNA was extracted from airway swab samples, and the V3-V4 region of the 16S rRNA gene was amplified using PCR. Sequencing was performed on the Illumina MiSeq platform using paired-end 300 bp reads.