Limosilactobacillus fermentum
The library was prepared following the native barcoding kit 24 V14 protocol, utilizing the SQK-NBD114.24 kit (ONT, Oxford, United Kingdom) with some modifications included extending the incubation period for both end-repair and ligation steps. To reduce DNA loss due to low input, a 1:1 ratio of DNA to AmpureXP beads was used. Additionally, a short fragment buffer was employed during the ligation wash step. No wash-step was introduced between the end-repair and barcode ligation stages.