16S rRNA metagenomic sequencing of Bos taurus: dung
Total DNA was extracted from fecal samples using the QIAamp PowerFecal Pro DNA Kit according to the manufacturers instructions with some modifications, two cycles at 70C for 5 min prior a bead-beating step of 2 cycles of 5 min at 26 hertz. The extracted DNA was checked on 1% agarose gel, and DNA concentration and purity were determined using a NanoDrop 2000 UVvis spectrophotometer  nd stored (-80C) until further processing. The V3-V4 hypervariable regions of the 16S rRNA gene were amplified from the extracted DNA using primers 341F (CCTAYGGGRBGCASCAG) and 806R (GGACTACNNGGGTATCTAAT). Sequencing was performed on an Illumina NovaSeq PE250 at Novogene.