GSM8647969: wt_15_ex_37; Mus musculus; RNA-Seq
RNA was extracted from gastrocnemius muscle (~30mg). Tissue was homogenised in Trizol (1 mL) using a steel bead with the Qiagen TissueLyser II (3 x 90 sec at 30 Hz; 4°C). Chloroform (200 mL) was added to all the samples and mixed by 30 seconds of shaking prior to centrifugation for 15 minutes at 12,000 g at 4°C. The aqueous phase was combined with an equivalent volume of 70% ethanol and thereafter RNA extraction was performed according to the manufacturers' instructions for the RNeasy Mini Kit (74,106, Qiagen). Libraries were prepared uisng 500 ng of RNA and the TruSe Stranded mRNA Library Prep assay kit, according to manufacturers instructions.