GSM8705459: HaCaT-Ras (-); Homo sapiens; RNA-Seq
Total RNAs were extracted from HaCaT-HRasG12V cells using Trizol reagent (Thermo Fisher Scientific). RNA quality was evaluated using Agilent Bioanalyzer with RNA 6000 Pico kit (Agilent Technologies Japan). The RNA sequencing library was prepared with Agilent Bioanalyzer, DNA High-sensitivity kit (Agilent Technologies Japan, Ltd.) and performed with Illumina NextSeq500 (Illumina K.K.).