GSM9364729: anti-H3K4me3; Saccharomyces cerevisiae; OTHER
Nuclei extracted from yeast cells (500K) were utilized in each reaction. CUT&RUN Libraries were prepared using CUTANA CUT&RUN Library Prep Kit. 5 ng  of DNA was used as the starting materail for all samples. Libraries were amplified using 14 PCR cycles on the thermocycler. Post amplificaiton libraries were purified using SPRI beads. Libraries were checked on an Agilent TapeStation. Samples concentrations were normalized based on concentration in 200-700bp region prior to sequencing.