whole genome
Whole-genome sequencing was performed using the MinIon MK1B(MIN-101B) platform with 5194750 bp (Oxford NANOPORE technologies). Library preparation was conducted using the Rapid barcoding kit 96V14(SQK-RBK114.96) (Oxford NANOPORE technologies). FLO-MIN114, MinIONor GridION Flow cell R10 version-lot 11004111 by Oxford NANOPORE technologies was loaded with prepared library. Resulting fast5 reads were base called using the high-accuracy base-calling algorithm in fast@v4.3.0. MinION reads (fastq les) were trimmed with NanoFilt v2.8.0, generating a read length (N50) 13,925.0, total bases 60,867,894.0, >Q10: