WGS of monoculture L. garvieae
The sequencing workflow of WT02 by CycloneSEQ consists of four steps: sample processing, libraryconstruction, real-time sequencing, and bioinformatics analysis.1)Sample processing.For sampleprocessing, we recommend using a third-party extraction kit or the CTAB method to manually extractnucleic acids. It is also acceptable to use third-party reagents to amplify the target sample DNA/cDNA.Forlong fragments of 50-150kb in length, Qiagen's Genomic-tip and Genomic DNA buffer set arerecommended. For ultra-long fragments >100kb, Qiagen Puregene kit and NEB Monarch High MolecularWeight DNA Extraction Kit (cells and blood) are recommended. NucleoBond HMW DNA kit isrecommended for plant genome extraction.2)Library construction.The library construction kits provided withthe CycloneSEQ platform can be used for library construction. The kits include library preparationreagent kits, barcode library preparation reagent kits, etc.3)Real-time sequencing.The sequencing reagentsand chips provided by the CycloneSEQ platform are used for sequencing on the WT02 sequencer andobtaining FastQ data in real time.4)Bioinformatics analysis CycloneSEQ data is compatible with a varietyof third-party tools for bioinformatics analysis to meet different application scenarios, giving users greaterflexibility in data analysis.