GSM9364912: Sample 9_III-5; Homo sapiens; RNA-Seq
Total RNA was extracted from the tissue from mice and purified with RNeasy Plus Mini Kit (Invitrogen, 12183018A). Ribosomal RNA was removed from 1ug of total RNAs and cDNA was synthesized using SuperScript III (Invitrogen). Libraries for sequencing were prepared according to the manufacturer's instructions with TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold (Illumina, RS-122-2301) and paired-end sequencing was done with a Nextseq 2000 (Illumina). Libraries for sequencing were prepared using standard New England Biolabs's protocols.