GSM8617975: KD_2; Mus musculus; RNA-Seq
Each sample is lysed using a 2 µL lysis buffer with 0.2% TritonX-100 and 40 U/mL Recombinant RNase Inhibitor, which disrupts cell membranes to release RNA and other cellular contents while preventing RNA degradation. Poly(A)-tailed RNA molecules in cells were processed using the Smart-seq2 protocol. Lysis of each sample in a specialized buffer, followed by reverse transcription and cDNA amplification for 16 cycles. Tagmentation with Tn5 enzyme mix and PCR was employed to build sequencing libraries, which were then amplified and purified. Library quality was assessed using PerkinElmer LabChip GX Touch.