RNA-Seq of FMDV isolate A-2167/Karachay-Cherkessia/2013
RNA was extracted from cell culture supernatants using Trizol reagent (Thermo Fischer Scientific, USA) in accordance with the manufacturer's instructions. Transcriptome libraries were prepared from 100500 ng of RNA using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, USA) using random primer-based protocol without prior enrichment. Sequencing was performed on a NovaSeq 6000 system (Illumina, USA) utilizing an SP flow cell with 2*100 bp paired-end reads.