GSM9012169: EBV-LCLs, CD46 KO, IFN-treated (biol rep 1); Homo sapiens; RNA-Seq
RNA was extracted using the RNeasy MicroKit (Qiagen), ), including on-column DNase digestion. Poly(A) mRNA was isolated using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB), and libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit (NEB). Libraries were quantified with Qubit dsDNA BR Assay (Invitrogen) and quality control was performed using High Sensitivity D1000 ScreenTape Assay on 4150 TapeStation System (Agilent).