GSM8668989: WT_HFD2; Mus musculus; RNA-Seq
Tota RNA was extracted using Trizol. Ribosomal RNAs were removed from total RNA with Ribo-Zero Gold rRNA Removal Kit (Illumina). Libraries were prepared using the CORALL RNA-Seq V2 Library Prep Kit (LEXOGEN), with mRNA isolated using the Poly(A) RNA Selection Kit (LEXOGEN). Indexed libraries were enriched by PCR, and quality was confirmed using TapeStation HS D1000 Screen Tape.