RNA-seq of MDA-MB-231 cells: FAM135B overexpressing
The mRNA was enriched using oligo(dT) magnetic beads, and the mRNA was fragmented by adding interrupting reagent to the enriched mRNA. The interrupted mRNA was used to synthesize one-stranded cDNA and two-stranded cDNA according to the corresponding procedures, and the whole library preparation was completed through the steps of end repair, addition of A-tail, addition of sequencing junctions, purification, PCR amplification, and product cyclization. After passing the library test, DNBSEQ, a BGI high-throughput sequencing platform, was used for sequencing.