GSM9009607: Mus musculus, Leydig cells, siprdx1+H2O2+FV, biol repl3; Mus musculus; RNA-Seq
The total RNA of cells in each group was extracted with Trizol (Thermo Fisher Scientific). The RNA with RNA integrity number (RIN) > 7.0 is acceptable for cDNA library construction. The cDNA libraries were constructed for each RNA sample using the TruSeq Stranded mRNA Library Prep Kit (Illumina, Inc.) according to the manufacturer's instructions. The libraries were quality controlled with Agilent 2200 and sequenced by NovaSeq 6000 on a 150 bp paired-end run.