GSM9041889: p1-sinc2; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol reagent (Invitrogen), following the manufacturer's instructions. RNA quality was assessed with Agilent 2100 Bioanalyzer and Qubit fluorometer. RNA libraries were constructed using the mRNA enrichment protocol with poly(A) magnetic beads, fragmentation, cDNA synthesis, end repair, A-tailing, adapter ligation, size selection, and PCR amplification. Libraries were validated with Agilent 2100 Bioanalyzer and sequenced using Illumina platform.