GSM9271163: SKVO3 cells, shEmpty - 2D - rep1; Homo sapiens; RNA-Seq
For RNA Sequencing,  SK-OV-3 cells (NR3C1 KD and CTR cells) were plated at high density (1.2 × 10³ cells/cm²) in 60mm plates and SK-OV-3 spheroids (NR3C1 KD and CTR cells) in ultra low-attachment plates and harvested ay day 6. RNA purification was performed using Trizol. mRNA liibraries were prepared with Illumina Stranded mRNA Prep (April-2021) following manufacturer's protocol.