Targeted capture of mtDNA of homo sapiens: blood
Genomic DNA was extracted from blood using either a phenolchloroform extraction method or an automated nucleic acid purification protocol following manufacturers guidelines (Freedom EVO Nucleic Acid Purification Workstation, PROMEGA kit Mag-neSil Genomic, Large Volume System). The quality and quantity of purified genomic DNA were evaluated spectrophotometrically (NanoDrop ND-1000 Spectrophotometer). Multiplex sequencing on the Illumina GAII platform after in-solution capture en-richment was used to obtain complete mtDNA genome sequences with an average of 352-fold coverage depth at the Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology (Leipzig, Germany).