Shotgun DNA sequencing of human gut metagenome
DNA was extracted using DNeasy PowerSoil Pro Kit (Qiagen), libraries were prepared using Illumina DNA prep - LP (M) Tagmentation reagents (Illumina) and sequenced on an Illumina NextSeq 500 with Illumina NextSeq 500/550 Mid Output Kit v2.5 (300 Cycles). Data is depleted of human sequences.