Illumina short reads of Opsariichthys pachycephalus
Genomic DNA was sheared using a Covaris 2 machine, and then quantified using a Qubit 2.0 Fluorometer before Illumina sequencing. A short-read sequencing library was also constructed from 200ng of the sheared gDNA using the TruSeq Nano DNA High Throughput Library Prep Kit (Cat. No.20015965). It was then sequenced using an Illumina NovaSeq 6000 through the GENOMICS BIOSCIENCE & TECHNOLOGY CO., LTD service based in Taipei, Taiwan.