RNAseq of rice Leaf
Total RNA was extracted from the collected shoot apical meristems (SAMs) using the TRIzol reagent (Invitrogen, Waltham, MA, USA), following the manufacturers protocol. The extracted RNA was enriched for mRNA to construct transcriptome libraries. Library preparation was performed using the VAHTS Universal V8 RNA-seq Library Prep Kit for Illumina (NR713; Vazyme, Nanjing, China), in accordance with the suppliers instructions.