RNA-seq of mus musculus: young cecum
RNA was extracted by Direct-zol RNA Miniprep kit (Zymo, R2050) and Trizol (Invitrogen, 15596026). To break the tissue, 2mm bead was added (Zymo, S6003) to extraction tube then beat at max speed for 6 minutes using a tissuelyser. RNA samples were then submitted to Advanced Genomic Core