Rat_PFC_control
DNA samples were fragmented into 200-400bp using Covaris S220. The DNA fragments were repaired, dA-tailed and ligated to sequencing adaptors that all cytosines were methylated. Then the DNA fragments were Bisulfite treated with EZ DNA Methylation Gold Kit (Zymo Research). After the treatment, Cytosines without methylation changed to U (after PCR amplification to T), while Cytosines with methylation remained unchanged.The final DNA library was obtained by size selection and PCR amplification.