GSM8882018: hiPSC cardiomyocytes, monoculture without TGF-Beta inhibitor, rep 2; Homo sapiens; RNA-Seq
Single-cell RNA sequencing was done following the supplier's manual for the Chromium Next GEM Single Cell Sequencing Kits. The 2D co-cultures were singularized using 0.25% trypsin-EDTA for 20 minutes at 37°C. The viability of the cells was determined via propidium iodide on the BD Accuri C6 flow analyzer and then the cells were prepped for fixed single-cell sequencing according to the Chromium Next GEM Single Cell Fixed RNA Sample Preparation Kit (10X Genomics 1000414). Libraries for single-cell sequencing were created using the Chromium Fixed RNA Kit, Human Transcriptome, 4 rxns x 4 BC (10X Genomics 1000475) by following the manual for probe hybridization and library creation. The samples were sequenced according to the 10X genomics FLEX single-cell sequencing workflow at the University of Minnesota Genomics Center (UMGC).