GSM9040324: CUT&TAG_sgNTC, D5; Mus musculus; OTHER
100,000 cells were bound to concanavalin A-coated magnetic beads, sequentially incubated with H3K27ac primary antibody overnight at 4°C, goat anti-rabbit secondary antibody for 1 hour at room temperature, and then treated with pA/G-Tn5 transposase complex for 1 hour at room temperature. Tagmentation was initiated at 37°C for 1 hour, terminated with SDS, and DNA fragments were purified using magnetic beads. DNA libraries for Cut&Tag assays were prepared using Hyperactive Universal CUT&Tag Assay Kit following manufacturer's protocols