chloroplast genome sequence of Lophophora diffusa
Stem tissue was collected from Lophophora diffusa muture plants. Total genomic DNA was extracted using the CTAB method. DNA purity and quantity were evaluated using the NanoDrop 1000 and Qubit from Thermo Fisher Scientific. A 15-kb library was constructed using a SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences, CA, USA). The construction included DNA shearing, AMPure PB bead purification, ssDNA overhang removal, damage repair, end repair, hairpin adapter ligation, and library bead purification. Following quality control, a SMRT bell library was obtained. The library was sequenced on the PacBio Revio platform (Pacific Biosciences, CA, USA). Raw data were processed using the CCS algorithm (version 6.0.0, parameters: min Passes 3  min Predicted Accuracy 0.99 max Length 21,000) to generate highly accurate HiFi reads.