GSM9092539: DC1, INP, rep1; Mus musculus; ChIP-Seq
Bone marrow was isolated from femur and tibia. pDC, DC1 and DC2 populations (N=3 biological replicates) were sorted by FACS. Cells from 5 (WT or KO) mice were pooled per replicate. N=2 pools with the highest cell count for each cell type were fixed with 1% methanol free formaldehyde for 10 minutes & quenched with 125 mM glycine for 5 minutes. Quenced cells were lysed in 50 mM Tris-HCl pH8, 10 mM EDTA pH8, 1 % SDS + protease inhibitor cocktail, and sonicated for 3 minutes (30sec on, 30 sec off) on the Bioruptor PICO. Yeast chromatin was spiked in (1:1000) based on DNA concentration) prior to immunoprecipitation to allow for downstream normalization. The library was prepped using the KAPA genomic DNA prep Hyper, purified and quantified using the Bioanalyzer DNA 7500.