Denovo assembly of Xuefeng Black-bone chicken: adult female liver
Select high-quality DNA samples with acceptable integrity (main band > 30 kb); Fragment the DNA randomly into pieces (15-18 kb) using a Covaris ultrasonicator; Enrich and purify large DNA fragments using magnetic beads; Perform damage repair and end repair on the fragmented DNA; Ligate stem-loop sequencing adapters to both ends of the DNA fragments, and use exonucleases to remove fragments with failed ligation; The constructed library is sequenced using the PacBio Sequel II platform.