GSM8842728: 2022.03.18-MN0-200; Homo sapiens; RNA-Seq
Cells pelleted and snap frozen, extracted using RNAqueous micro kit with DNase I treatment, followed by glycogen precipitation and resuspension in ultra-pure H2O. Libraries were prepared from total RNA using the SMART-Seq v4 Ultra Low Input RNA Kit and the Nextera XT DNA Library Preparation Kit. Library size distribution was validated using an Agilent 4200 TapeStation.  Additional library QC, blending of pooled indexed libraries, and cluster optimization was performed using Life Technologies' Invitrogen Qubit® 2.0 Fluorometer.