GSM8979089: ZNF729-FH_24h_REP3; Homo sapiens; RNA-Seq
RNA was extracted with Trizol and purified with Direct-zol columns Messenger RNA was purified using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. Libraries were prepared by end repair, A-tailing, adapter ligation, size selection, amplification, and purification and they were checked with Qubit and qPCR for quantification and Bioanalyzer for size distribution detection.