GSM8745888: Over-expression empty vector, inoculated soybean cyst nematode, Day3, biol rep 3; Glycine max; RNA-Seq
Total RNA was extracted using Trizol reagent (thermofisher, 15596018). RNA quantity and purity were analyzed using Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA, 5067-1511) Library construction for mRNA sequencing was conducted using the SuperScriptTM II Reverse Transcriptase (Invitrogen, cat. 1896649, USA) following the manufacturer's recommendations