RNA-seq of wheat tissues related to receptivity
At each sampling point, four spikelets from the middle of spikes from all the plants were harvested and total RNA was extracted using Spectrum Plant Total RNA isolation kit (Merk KGaA, Darmstadt, Germany) according to the manufacturers instructions. DNA was removed by digestion with Turbo DNase protocol (Thermo Fisher Scientific Australia Pty Ltd, Scoresby, Australia). Library construction was done with TruSeq Stranded Total RNA protocol and sequencing was done with Illumina NovaSeq 6000 (150 bp paired-end setting)