GSM9364363: Sarcoma, shLpar4, abemaciclib, rep 3; Mus musculus; RNA-Seq
RNA was extracted from cells using TRIzol. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis; then end repair, A-tailing, adapter ligation, size selection, amplification, and purification.