GSM9364694: Dex2; Mus musculus; OTHER
CUT&RUN was performed using the EpiCypher (V4) kit according to manufacturer instructions from 500,000 cells. Permeabilization was performed using 0.01% Digitonin and 0.5ug of Glucocorticoid Receptor antibody (CST #12041) was used for overnight immunoprecipitation. E.Coli Spike-in DNA was added after chromatin digestion. Resulting DNA was submitted to GENEWIZ for library construction and sequencing using Nova-Seq 2x150bp chemistry.