XR_1R1 (CRX1628680; CRA024785)
The total DNA was extracted from 0.5 g of freeze-dried samples according to the manufacturer's protocol using the DNeasy PowerSoil Total DNA Kit (12888-100, QIAGEN, Germany). The concentration and purity of the extracted DNA were subsequently assessed using a Nanodrop 2000 spectrophotometer (ND2000, Thermo Scientific, USA). Following successful quality control evaluation, full-length 16S rRNA amplicon sequencing for bacteria and archaea was conducted on the PacBio Sequel platform to determine microbial community composition at the species level. The primer sets for amplifying the bacterial 16S rRNA gene and archaeal 16S rRNA gene were 27F (5'-AGAGTTTGATCMTGGCTCAG-3')/1492R (5'-ACCTTGTTACGACTT-3') submitted_instrument_model: PacBio Sequel