GSM8619044: Hippocampus, 5xFAD, replicate 2; Mus musculus; RNA-Seq
total RNA was initially subjected to ribosomal RNA (rRNA) depletion through the use of the GenSeq® rRNA Removal Kit (GenSeq, Inc.), in accordance with the instructions provided by the manufacturer. Subsequently, the rRNA-depleted samples were processed for library construction using the GenSeq® Low Input RNA Library Prep Kit (GenSeq, Inc.). The quality and quantity of the library were evaluated using the Agilent Bioanalyzer 2100 system (Agilent Technologies, Inc., USA). The sequencing was conducted on an Illumina NovaSeq platform, resulting in the generation of 150 bp paired-end reads.