GSM9011398: Cannabis sativa L. Leaf, Putrescine 0.9mM treatment, RNA-seq, R1; Cannabis sativa; RNA-Seq
Leaves collected for transcriptome analysis were immediately frozen in liquid nitrogen. Total RNA was extracted using the Rneasy Micro Kit (Qiagen, Hilden, Germany), and RNA concentration was measured with the Quant-iT™ RiboGreen RNA Assay Kit (ThermoFisher Scientific Inc., Waltham, MA, USA). RNA integrity was asseessed on an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), and samples with an RNA integrity number (RIN) of 7.0 were used. mRNA libraries were prepared using the TruSeq Standard mRNA Library Prep Kit (Illumina Inc., San Diego, CA, USA).