GSM9039754: [bEnd.3][Norrin][replicate3]; Mus musculus; RNA-Seq
Trizol (Invitrogen, 15596026) was used to extract total RNA. DNA was digested (Turbo DNAse free, Invitrogen™ AM1907), and RNA was ribodepleted (Ribozero RNA removal kit, Illumina). The samples RNA integrity values were >8. Libraries were made using the TakaraBio SMARTer Pico Mammalian kit. Novaseq 150PE was carried out, generating approximately 45 million reads per sample.