Amplicon of CO1: mushroom fruiting body
DNA extracted from mushroom fruiting bodies using modified salt extraction protocol (Aljanabi, S. M. & Martinez, 1997) followed by library preparation using the Illumina protocol. A 157bp fragment was amplified using the primers ZBJArt1c and ZBJArt2c (Zeal et al, 2011). All locus-specific primers were modified to include Illumina adapters. Reverse primers also included a unique 12 bp Golay barcode, primer pad (AGTCAGTCAG) and linker (CC) (Caporaso, J. G. et al, 2012).