GSM9151616: KO; Mus musculus; RNA-Seq
Mice were transcardially perfused with ice-cold PBS. Brains were dissected and dissociated using the Adult Brain Dissociation Kit (Miltenyi Biotec) and gentleMACS Octo Dissociator. CD11b⁺ cells were isolated using BD IMag magnetic particles. Single-cell suspensions were processed using Chromium Next GEM Single Cell 3′ Reagent Kit v3.1 (10x Genomics, Cat# PN-1000268) and Chip G on a Chromium Controller. GEMs were generated, followed by reverse transcription, cDNA purification, amplification, and library preparation according to 10x Genomics protocol (CG000315 Rev E).