GSM9364898: Input splenic B220+ (Miz-1DPOZ); Mus musculus; ChIP-Seq
10 million cells were fixed with 1% Formaldehyde for 10 minutes with agitation at room temperature. Fixation was blocked by the addition of 125 mM glycine for 5 minutes with agitation at room temperature. Protein-chromatin complexes were collected using dynabeads overnight with agitation at 4°C. Complexes bound beads were then washed and eluted from beads for 4h at 65°C. RNAse and proteinase K were added to the mix for 1h at 42°C. DNA was extracted using QIAquick kit (Qiagen). DNA was then used to prepare librairies (KAPA Hyper Kit) for NGS sequencing (pair-end, 50 cycles) on HiSeq 2000/2500 System (Illumina).