GSM8809596: nucleus pulposus cell-derived exosomes_1; Rattus norvegicus; miRNA-Seq
Total RNAs were isolated using the Total Exosome RNA Isolation Kit (Thermo Scientific) and miRNA sequencing was conducted by LC-Bio Technology Co., Ltd. (Hangzhou, China). The quality and quantity of total RNA were assessed using a Bioanalyzer 2100 (Agilent) with an RIN number >7.0. Small RNA libraries were prepared from approximately 1 μg of total RNA according to the TruSeq Small RNA Sample Prep Kits (Illumina) protocol.