GSM9270500: WT_biol rep 1; Mus musculus; ATAC-seq
EL4 tumor cells were injected into WT and Sphk2 KO mice. After 14 days, tumor-derived MDSCs (FACS-sorted Gr1+CD11b+ cells from ascitic fluid) were collected from WT and Sphk2 KO mice and used for making ATAC-seq libraries. We used 2 × 10^4 MDSCs and modified the concentration of the Tn5 enzyme for the transposase reaction (PMID: 35454405). NEBNext Ultra II DNA Library Prep Kit for Illumina from NEB was used to prepare libraries following manufacturer's protocol.