GSM9009589: PC_RNA#3; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNeasy Mini Plus Kit (Qiagen) following the manufacturer's instructions. RNA libraries for circRNA sequencing were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina, with modifications to enrich for circRNAs following RNase R treatment. Libraries were constructed according to the manufacturer's protocols.