Virome sequencing and analysis of Aedes aegypti and Aedes albopictus from ecologically different sites in the Philippines
Virome sequencing was performed on viral RNA extracted from pooled Aedes aegypti and Aedes albopictus mosquito samples. Total viral RNA was amplified using random PCR or SISPA (Sequence-Independent, Single-Primer Amplification), followed by library preparation. Sequencing was conducted on the Illumina MiSeq platform using paired-end sequencing layout to characterize the complete viral metagenome present in both laboratory-reared and field-collected mosquito populations from three collection sites in the Philippines (Bagong Silang, Lalakay, and Bayog).