GSM9041537: C57BL/6 Cholangiocytes, Control, Replicate 2; Mus musculus; RNA-Seq
Wildtype male liver tissue were dissociated (#130-096-730, Miltenyi Biotech, Germany). CD326 (EpCAM) Microbeads (#130-105-958, Miltenyi Biotec, Germany) were used to isolate the cholangiocytes by immunomagnetic separation using MACS columns. To increase the purity of the cholangiocyte population, the positively selected cell population was separated over a second column. Direct-zol RNA MicroPrep Kit (#R2060, ZYMO, CA, USA) was used to isolate total RNA from the CD326+ cells. cDNA libraries were prepared and sequenced by the Genome Analysis Core (Mayo Clinic, Rochester, MN, USA) using Illumina Stranded mRNA Prep following manufacturer recommendations.