16S rRNA amplicon sequencing of human vaginal microbiome from Putian (Z72_12)
PCR amplification of the V3V4 hypervariable region of the 16S rRNA gene using primers 341F (5-CCTACGGGNGGCWGCAG-3) and 806R (5-GACTACHVGGGTATCTAATCC-3). Amplicons were purified with Agencourt AMPure XP beads (Beckman Coulter), indexed using the Nextera XT Index Kit (Illumina), quantified by Qubit dsDNA HS Assay (Thermo Fisher), pooled equimolarly, and sequenced on an Illumina NovaSeq 6000 system with 2150 bp chemistry.