ONT reads for GP
The whole genomic DNA was extracted by the SDS method, purity and integrity were subsequently detected by agarose gel electrophoresis, and concentration was measured by Qubit. For the sequencing of ONT ultra-long reads, large fragments of DNA were recovered and end-repaired, 20 K libraries were constructed according to standard instructions from Oxford Nanopore Technologies, and final sequencing was performed by the Nanopore platform.