Pacbio HiFi sequencing data of Polymita picta
DNA concentration and DNA fragment length were assessed using the QuantiFluor ONE dsDNA system on the Quantus Fluorometer (Promega Corporation) and the Genomic DNA Screen Tape on the Agilent 4150 TapeStation system (Agilent Technologies), respectively. We prepared one PacBio ultra-low input library including a long-range PCR amplification step using the SMRTbell gDNA Sample Amplification Kit and the SMRTbell Express Template Preparation Kit 2.0. In addition, to reduce potential PCR biases of the amplification polymerases supplied by PacBio, we prepared one further library using the KOD Xtreme Hot Start DNA Polymerase (Merck), optimized for amplification of long and GC-rich DNA templates. For this, we combined the buffer, dNTPs and KOD polymerase from the KOD Xtreme Hot Start DNA Polymerase Kit with the ultra-low input primers from the PacBio SMRTbell gDNA Sample Amplification Kit.