GSM8881779: SNF2L-IAA_2; Toxoplasma gondii; OTHER
CUT&Tag was used to identify SNF2L occupancy in the genome of SNF2L-mAID strain. Intracellular parasites are released through needle channels and purified by 3 µm pore size membrane filtration. Approximately 5 × 105 Toxoplasma gondii tachyzoites were collected and processed using Illumina's Hyperactive Universal CUT&Tag Assay Kit (Vazyme Biotech, China) according to the manufacturer's instructions. Mouse anti-HA was used as primary antibody for immunoprecipitation. The resulting DNA was amplified using TD 202 TruePrep Index Kit V2 for Illumina (Vazyme Biotech, China) for library construction. The libraries were enriched, quantified and sequenced using the PE150 model on a NovaSeq 6000 sequencer (Illumina).