DAP-Seq of Triticum aestivum: Input
The fusion protein was expressed in vitro using the TNT SP6-coupled wheat germ extract system (Promega). Proteins expressed were directly captured using Magne Halo Tag Beads (Promega) and incubated with the genomic DNA library. Protein-bound DNA samples were sequenced on the Illumina HiSeq 2500, with single-end reads measuring 150 bp.