RNA-Seq of Pseudomonas aeruginosa: 4 mM Glyoxal 60 minutes Rep2
Samples were rRNA depleted and cDNA libraries synthesized from RNA by random priming with UTP incorporation. 3' adenosine tails were added followed by adaptor ligation, dUTP strand degradation, and PCR amplification. Samples were sequenced on a Illumina HiSeq X.