delta_ralT_1
Total RNA was extracted with a High Pure RNA Isolation kit (Roche Diagnostics, Mannheim, Germany). Ribosomal RNA was eliminated from the extracted total RNA using a Ribo-Zero rRNA Removal kit (Gram-negative bacteria) (Illumina, Madison, WI, USA) as previously described (Hayashi et al., 2019a).The libarary was prepared with MGIEasy Circularization Kit (MGI Tech Co., Ltd.).