WGS of YR_13: Rice prebreeding line F2 of cross O. sativa cv. Yamuna x O. rufipogon
Library preparation was performed using QIASeq FX DNA Library Preparation protocol. About 40 ng of Qubit quantified DNA was enzymatically fragmented, end-repaired and A-tailed in a one-tube reaction using the FX Enzyme Mix provided in the QIASeq FX DNA kit. The end-repaired and adenylated fragments were subjected to adapter ligation, whereby index-incorporated Illumina adapter was ligated, to generate sequencing library. The library was subjected to 6 cycles of Indexing-PCR (Initial Denaturation at 98 C for 2 min, cycling (98 C for 20sec, 60 C for 30sec, 72 C for 30sec) and final extension at 72 C for 1min) to enrich the adapter-tagged fragments.Finally, the amplified library was purified using Cambrian Bioworks CamSelect NGS (#CBWD010) followed by library quality control check. Illumina-compatible sequencing libraries  were quantified by Qubit fluorometer and its fragment size distribution was analyzed on Agilent 4150 TapeStation.