GSM8809943: HUDEP-2, control gRNA, sample September1; Homo sapiens; RNA-Seq
Following a fluorescence-based sort of transduced cells (> 99%), purity was monitored and RNA samples were isolated using RNAeasy Plus Kit (Qiagen) the between four and seven months after transduction; RNA integrity was verified using  Agilent 2100 Bioanalyzer trace analysis (Agilent Technologies). 25 total RNA were processed using the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (New England Biolabs) as per manufacturer's instructions. cDNA (20 ng input standardized for fragmentation, end repair, 5' phosphorylation and dA tailing followed by adapter ligation) and final library quality was monitored using Agilent 2100 Bioanalyzer trace analysis (Agilent Technologies). Pooled libraries were sequenced on an Illumina NovaSeq X Plus 10B (2x150) flow cell to a depth of 100 M read pairs (150 bp) per sample at Genome Technology Access Center (GTAC) and McDonnell Genome Institute at the Washington University School of Medicine.