RNA-seq of Ae. albopictus: CHIKV-infected midgut day5
The libraries were prepared in accordance with the Illumina TruSeq Stranded mRNA Sample Preparation Guide for Illumina Paired-End Indexed Sequencing and then validated using the Fragment Analyzer to check the distribution. Finally, concentration of library samples was defined based on the Qubit 3.0 Fluorometer quantification. Indexed DNA libraries were normalized to 2 nM and then pooled in equal volumes. The pool was loaded at a concentration of 1.2 pM onto an Illumina NextSeq 550 Flowcell High Output, with 1% of Phix control. The samples were then sequenced using the Illumina chemistry V2, 2x75 bp paired end run.