GSM8844682: Cancer cells from KPC- Grin2d -/-  Mouse Nr 23; Mus musculus; RNA-Seq
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser. RNA libraries were generated using the SMARTer mRNA-Seq Library Preparation Kit (Takara Bio), following the manufacturer's instructions. Eukaryotic 250~300 bp insert cDNA library Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection Illumina NovaSeq X Plus sequencer and 25B sequencing flow cell, the sequencing strategy employed is PE150.