GSM8842478: Output pool, covST282A, Biological Replicate 2; Streptococcus agalactiae; OTHER
Plasmid purification were done with QIEprep Spin Miniprep (Qiagen) following instructions for large vector (>10kb). PCR amplification (25 cycles) were done with Phusion+ high fidelity polymerase (NEB). PCR products were gel purified (QIAquick PCR, Qiagen). Illumina adaptaters were added (PCR-free) following provider workflow (Novogen).