Shotgun metagenomic sequencing of pericardial fluid from a hyacinth macaw (Anodorhynchus hyacinthinus)
DNA was extracted from pericardial fluid from a hyacinth macaw (Anodorhynchus hyacinthinus) using the ZymoBIOMICS-96 MagBead DNA Kit (D4302, Zymo Research) on an automated platform. For some low biomass samples, the ZymoBIOMICS DNA Microprep Kit (D4301, Zymo Research) was used to allow lower elution volumes and higher DNA concentrations.Sequencing libraries were prepared from the extracted DNA using the Illumina DNA Prep Kit with 10 bp unique dual indexes, following the manufacturers protocol. Libraries were quantified with Qubit, pooled at equal abundance, and the final pool was quantified using qPCR. Shotgun metagenomic sequencing was performed on an Illumina NovaSeq X platform with paired-end 150 bp reads.