GSM8908355: OTX2-dTAG_PS_HA_Rep3; Mus musculus; OTHER
CUT&RUN on OTX2-HA was performed on 500,000 EpiSCs, PS or DE cells per sample using BioMag Plus Concanavalin A beads (ConA beads, PolySciences NC1358578), 1:100 anti-HA (CST C29F4 #3724) or anti-IgG (EpiCypher 130042), and ProteinA/G MNase (EpiCypher). Fragmented DNA was isolated by phenolchloroform extraction and EtOH precipitation CUT&RUN libraries were constructed using the Takara Thruplex Library Prep and Unique Dual Index kits. Cycles for PCR amplification were determined individually for each sample by qPCR. Libraries were size selected using SPRI beads (Beckman Coulter)