GSM9364619: GFP_Pool129-2_13; Mus musculus; RNA-Seq
Cells were FACS-sorted on AriaIII (BD) as single cells/DAPI-/CD45-/GFP+ or Thy1.1+. GFP and Thy1.1 samples from different lungs were pooled after sorting for sequencing. Sorted cells were washed and resuspended in PBS+0.04% BSA (400 µg/ml) buffer at cell concentration of 1000 cells/µL. Libraries were prepared using the 3' v3.1 HT kit from 10x Genomics following manufacturer's instructions with 10,000 cell recovery and sequenced at 50,000 reads/cell.