GSM8705522: C04, tumor; Homo sapiens; RNA-Seq
RNA was isolated from the cells using TRIzol reagent (Invitrogen). We used 100 ng total RNA from all subjects to prepare sequencing libraries with by using the TruSeq stranded mRNA sample preparation kit(Illumina, CA, USA). Quality of these cDNA libraries was evaluated with the Agilent 2100 BioAnalyzer (Agilent, CA, USA). They were quantified with the KAPA library quantification kit (Kapa Biosystems, MA, USA) according to the manufacturer's library quantification protocol. Following cluster amplification of denatured templates, sequencing was progressed as paired-end (2×101bp) using Illumina NovaSeq6000 platfrom.