GSM9414742: BACH1; Mus musculus; RNA-Seq
CD-1 mouse retinas were dissected, GFP-positive punches extracted, and retina dissociated as previously described (Fadl, et al. Mol Vis 2020). One sample from each condition was used. Dissociate was used as input for 10X Genomics Chromium controller using v3.1 (Chromium Next GEM Single Cell 3ʹ GEM, Library and Gel Bead Kit v3.1) chemistry. Approximately 17,000 live cells per sample were loaded into the Chromium chip following the Cell Suspension Volume Calculator Table to capture transcripts from approximately 10,000 cells. Libraries were performed according to the manufacter's instructions (Single Cell 3' v3.1 protocol, 10x Genomics)