Caloric restriction rejuvenates myofiber growth in heart failure
Caloric restriction rejuvenates myofiber growth in heart failure
The strain of rats used in the experiment was ZSF1 purchased from Charles River (https://www.criver.com/products-services/find-model/zsf1-rat?region=3671). Rats in HFpEF_CR group were treated with dietary caloric restriction. Extensor digitorum longus (EDL) muscle was collected and transferred to liquid nitrogen until RNA extraction.  RNA extraction of the EDL was performed using the Trizol-chloroform-isopropanol method following Trizol reagent solution user guide (ThermoFisher Scientific, Cat No. AM9738).  Novogene, Cambridge, United Kingdom, performed RNA sequencing (RNA-seq). The library preparation was constructed using the TruSeq Stranded mRNA kit (Illumina), following the manufacturerâ€™s recommended protocol. The libraries were sequenced on the HiSeq 4000 platform with 150 bp paired-end strategy.