Tn-seq of WT Vibrio cholerae: T0
gDNA  Covaris E220 shearing (5/200/105, 40 s)  0.6 Ampure XL (3001000 bp)  3 poly-C tailing (rTdT, 37 C, 1 h)  PCR1 (biotin pSC189-PCR1 + olj376; Q5)  streptavidin bead capture  PCR2 (pSC189-PCR2 + BCXXXX; Q5)  Ampure cleanup  Illumina NextSeq 2000 (ReadTnLpSC189)