GSM8809909: UPN117_F04740-GEX; Homo sapiens; RNA-Seq
Baseline tumor tissue samples were obtained from 41 patients with recurrent/refractory malignant glioma participating in a Phase I study (IRB #13384) on cellular immunotherapy using central memory-enriched IL13Rα2-targeting Chimeric Antigen Receptor (CAR) T cells. Single-cell sequencing of cryopreserved freshly dispersed tumor samples from 41 individuals was carried out using the 10x Chromium platform. For Batch 1, patient PBMC's exomes were collected via Illumina exome panel and sequenced at 20M read pairs per patient for sample deconvolution. For batches 2-6 and batches 42-1, 42-2, 43-1, 43-2, 44-2, 45, 46, and 47, Biolegend Total Seq-C hashtag antibodies were used to allow sample deconvolution after pooled processing, with barcoded samples sorted at equal proportions into a single collection tube. Samples in the remaining batches were sequenced without pooling. 60,000 cells were loaded to a single Gel Bead-in-Emulsion (GEM) reaction onto the Chromium instrument. Sc-RNA-seq library preparation was performed according to manufacturer protocols and sequenced on Illumina iSeq100 for cell count validation and NovaSeq6000 at the recommended depth.