muscovy ducks cecum viral metagenome
After obtaining pure viral RNA, reverse transcription and cDNA library construction used the TruSeq Stranded mRNALT Sample Prep Kit of the Illumina. Then, the library quality inspection was done with Agilent High Sensitivity DNAKit on the Agilent Bioanalyzer machine, and the libraries were  quantified using Quant-iT PicoGreen dsDNAAssay Kit on the Promega QuantiFluor.