GSM9249363: ISO+L-NAME_1; Mus musculus; RNA-Seq
Total RNA was isolated from heart tissues using Trizol Reagent (Thermo Fisher Scientific, Cat# 15596026) according to the manufacturer's protocol, with glycogen (Roche, Cat# 10 901 393 001) added as a carrier. Libraries were prepared using the QuantSeq 3′ mRNA-Seq V2 Library Prep Kit (FWD) with UDI (LEXOGEN, Cat# 193.384) according to the manufacturer's instructions, for sequencing on the Illumina NextSeq platform.