GSM9363944: V. cholerae C6706 cells grown in Brain Heart Infusion containing extracts, replicate 3; Vibrio cholerae C6706; RNA-Seq
Bacterial RNA was stabilized using RNAprotect Bacteria Reagent (Qiagen) according to the manufacturer's instructions. Bacterial cells were pelleted by centrifugation, and total RNA was isolated using the SV Total RNA Isolation System (Promega), following the manufacturer's recommendations. RNA samples were treated with Turbo DNase (2 U/μL; Life Technologies) to eliminate residual DNA, followed by an additional purification step using the SV Total RNA Isolation System (Promega), according to the manufacturer's recommendations. RNA concentration and purity were assessed using an ND-1000 NanoDrop spectrophotometer (Thermo Fisher Scientific). Absence of DNA contamination in the RNA was checked by quantitative Real-Time PCR. RNA sequencing (RNA-seq) was performed on an Illumina NextSeq 2000 platform using a P1 flow cell, as single reads (100 bp), at The University of Kansas Genome Sequencing Core.