Comparison of transcriptomes in PBMCs between Griscelli-like patient and healthy controls by RNA-seq
Comparison of transcriptomes in PBMCs between Griscelli-like patient and healthy controls by RNA-seq
PBMC were isolated from total EDTA blood using density gradient centrifugation (Pancoll #P04-67500, PAN-Biotech GmbH, Aidenbach, Germany or Ficoll, CMSMSL01-01, Eurobio scientific, Courtaboeuf, France) RNA was extracted sing RNeasy® Mini Kit and QIAamp® RNA Blood Mini Kit (Qiagen, Hilden, Germany) SMARTer® Stranded Total RNA-Seq Kit v2 (Takara, Otsu, Shiga, Japan)