GSM8978648: C4-2, siMETTL3, direct RNA, rep 1; Homo sapiens; RNA-Seq
The total RNA was first extracted using RNeasy Plus Mini Kit (Qiagen), followed by mRNA purification using PolyATtract mRNA Isolation System (Promega). 500 ng mRNA was subjected to library construction using an Oxford Nanopore direct RNA sequencing kit (SQK-RNA002) according to the manufacturer's manual.