GSM9092559: Fenofibrate_50_3; Mus musculus; RNA-Seq
Total RNA was extracted using RNeasy Micro Kit (Cat#74004, Qiagen)following the manufacturer's instructions and checked for a RIN number to inspect RNA integrity by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US).Qualified total RNA was further purified by RNAClean XP Kit (Cat A63987, Beckman Coulter,Inc.Kraemer Boulevard Brea, CA,USA)and RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH, Germany). Purified total RNA was subjected to mRNA enrichment, fragmentation, and cDNA synthesis (first- and second-strand). The resulting double-stranded cDNA underwent end repair, 3'-end adenylation, and adapter ligation. Libraries were then enriched by PCR amplification and quantified using a Qubit® 2.0 Fluorometer with the Qubit™ dsDNA HS Assay Kit.