GSM8810144: Ythdf24OHT-6D-2; Mus musculus; RNA-Seq
Total RNA was extracted with RNeasy Mini Kit (QIAGEN, 74104) according to the manufacturer's instruction. mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.