GSM9149614: Experiment 2, Primary mouse astrocyte, 6 hour treatment, IL-1ɑ, rep2; Mus musculus; RNA-Seq
Cultured primary cells were rinsed once with ice-cold PBS and collected in freshly prepared extraction buffer consisting of 1% β-mercaptothanol in RLT (Qiagen). Total RNA was extracted using the RNeasy Mini Kit with on-column DNase treatment (Qiagen). RNA libraries were prepared using Truseq Standard mRNA Sample Prep (Poly A selection) following manufacturer's instructions (Document #1000000040498v00).