GSM9363192: MYCN-amp/FGFR1-N546K, mouse17; Mus musculus; RNA-Seq
RNA isolation from fresh-frozen tissue was performed with TRIzol (Ambion). RNA concentration was measured by QubitTM Assay (Invitrogen) and quality was assessed by 2100 Bioanalzyer (Agilent). Libraries were prepared using the Illumina Stranded TruSeq RNA sample preparation Kit. After library validation and quantification (Agilent Tape Station), equimolar amounts of library were pooled. The pool was quantified by using the Peqlab KAPA Library Quantification Kit and the Applied Biosystems 7900HT Sequence Detection System.