GSM9271377: Lgk974_24hr_HPAFII_ATAC_SEQ_SAMPLE_4; Homo sapiens; ATAC-seq
In brief, 50,000 cells were lysed with 100 µL ice- cold ATAC Lysis Buffer and centrifuged at 500xg for 10 min at 4°C. Tagmentation reaction was done by adding 50 µL tagmentation mix followed by incubation for 30 minutes at 37 °C in a thermomixer at 800rpm. Tagmentation product was cleaned up according to the manufacturer's suggestion. 33.5 µL of transposed product was used for library prep indexing PCR and post PCR cleanup was performed by 1.2x SPRI Select Reagent and final libraries were eluted in 20.5 µL DNA Elution Buffer.