Deep sequencing of PVC-treated mammalian cells
To detect PVC-induced genomic edits in target cells, we first amplified the target region out of each gDNA extract, barcoded the amplicons with indexed Illumina P5 and P7 NGS primers, purified libraries with a Qiagen PCR purification kit, quantified DNA yield on a NanoDrop instrument, and sequenced on an Illumina MiSeq instrument (with read length set to 300bp).