Customized_genome-wide_sgRNA_omission library
Original Customized genome-wide sgRNA omission library used for the screen. The 190bp library for sequencing was generated by PCR from the maxipreped lentiCRISPR library using primers GF3 (5-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNTAGTCTTGTGGAAAGGACGAAACACCG-3) and GRfixed (5-CAAGCAGAAGACGGCATACGagatACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAAC-3).