GSM8778048: VU_ILD_89; Homo sapiens; RNA-Seq
Single cell suspensions were generated using collagenase/dispase digest (1 mg/ml each) using a Gentlemacs Auto-dissociator. After serial filtering, column-based sorting of CD45+ cells (Miltenyi Microbeads) was performed, and CD45+ and CD45- fractions were mixed 1:2 for scRNA-seq library preparation. 10X Genomics 5' v1