RNAseq, Mus musculus, rostral cortex, 2 weeks off dox + 6 weeks ON dox (recovery), rNLS8, replicate 1
Rostral cortex (brain) whole tissue homogenisation > Total RNA extraction and purification > PolyA mRNA enrichment > mRNA fragmentation and synthesis of cDNA > end repair and dA-Tailing > purification of cDNA > PCR amplification > library validation > Illumina sequencing, paired-end 150 bp.