RNA-seq of croS-R1_R3
Exponential cultures of Efm5 and all the CPH high-MIC variants were grown on MHB without antibiotics at 37C and 150 rpm. Total RNA was purified using the RNeasy Mini Kit (Qiagen) according to the manufacturers instructions. RNA library preparation was performed using the Illumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus kit and 10bp unique dual indices. Sequencing was done on a NovaSeq X Plus (SeqCenter, USA), producing paired-end 150bp reads.