GSM8669744: ATAC-M6; Mus musculus; ATAC-seq
Processed primary cells were subjected to transposition reactions using the TruePrep DNA Library Prep Kit V2 for Illumina (TD501) and TruePrep Index Kit V2 for Illumina (TD202) according to the manufacturer's instructions. Nuclei from 5 × 10⁴ cells were pelleted and incubated with Tn5 transposase at 37 °C for 30 minutes. The resulting transposed DNA fragments were purified using the MolPure® PCR Purification Kit. Purified DNA fragments were amplified using 1× NEBNext High-Fidelity PCR Master Mix (New England Biolabs). The resulting library was further purified with the MolPure® PCR Purification Kit, quantified using a Qubit Fluorometer, and analyzed on an Agilent 2100 Bioanalyzer. Sequencing was performed on the Illumina Novaseq6000 platform using a paired-end 150 bp (PE150) strategy.