GSM8842644: F3382: gckp_rep2; Mus; RNA-Seq
RNA extraction made with the Maxwell® 16 LEV simple RNA Purification Kit (Promega, AS1280) and the Maxwell® 16 instrument (AS2000). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.