ChIP-seq of mouse peritoneal macropage: Hypoxia 4hrs
We collected TEPMs at 4  hours after 1% Oxygen. The TEPMs  were cross-linked for 10 min using 1% paraformaldehyde and then subjected to fragmentation using Sonifier (Branson, Dansbury, USA). We immunoprecipitated samples using the HIF-1 antibody (NB100-134, Novus Biologicals) .