cytb metabarcoding of filtered stream water: eDNA field sample
eDNA extracted using a modified Qiagen Blood and Tissue protocol from a Smith Root 5 micron self preserving filter was amplified with cytb metabarcoding primers (Stepien et al. 2020) targeting fsh species with Nextera stye overhangs, and dual indexed with Nextera indices following the Illumina 16S metagenomics protocol (Illumina Part# 15044223 Rev B). Samples were 250 bp paired-end sequenced on an Illumina MiSeq v3.