RNA-seq of Abies marocana: stem (pool)
Abies marocana saplings were subjected to seven treatments (control, soft cold, extreme cold, heat, drought, exposure to growth hormones, and physical damage) in control growth chambers. Total RNA was extracted from 100 mg of each sample using SpectrumTM Plant Total RNA Kit (Sigma-Aldrich, USA). Samples were then pooled by organ (at least 1000 ng of RNA in each pool, adding the same amount of each sample). mRNA libraries were constructed and sequenced at Macrogen (Korea).