RNA-Seq of tambaqui subjected to PBS: Control
After F. oreochromis infection, the samples were clustered into one pool of individuals belonging to the control group. Each pool consisted of 5 skin samples, resulting in 1 library for RNA sequencing. The RNA was extracted with TRIzol reagent (Invitrogen). Total RNA quantification was performed using the Qubit RNA kit on a Qubit 2.0 Fluorometer (Life Technologies). The integrity was verified by 1.5% gel electrophoresis, identifying the 28S and 18S bands, respectively. Sample purity was assessed using the NanoDrop One (ThermoFisher).