WGS of Klebsiella pneumoniae: 10 days, Female, Blood
KAPA Hyper prep kit for Illumina was used to prepare libraries for whole genome sequencing. 50ng of DNA per sample was fragmented to ~350bp and then the sheared DNA was checked for the fragment distribution. Sheared DNA was then end repaired followed by A-tailing and adapter ligation. Adapter ligated products were then purified followed by size selection and then enriched using PCR. PCR products were then purified and the final libraries were checked for fragment size distribution on Fragment Analyzer. Prepared libraries were quantified using Qubit dsDNA High Sensitivity Assay. The obtained libraries were pooled and diluted to final optimal loading concentration before cluster amplification on Illumina flow cell.