GSM9249796: HeLa cells : DTP selected following IC90 Etopiside drug treatment for 7 days_1; Homo sapiens; RNA-Seq
total RNA was extracted using RNeasy Mini Kit (Qiagen), according to the manufacturer's protocol Libraries were prepared using Roche Kapa Biosystems RNA HyperPrep sample preparation reagents on a Beckman Coulter Biomek i7. Finished dsDNA libraries were quantified by Qubit fluorometer and Agilent TapeStation 4200. Uniquely dual indexed libraries were pooled in an equimolar ratio and shallowly sequenced on an Illumina MiSeq to further evaluate library quality and pool balance