16S of Homo Sapiens : stool
Stool DNA was extracted from DNA/RNA Shield samples using ZymoBIOMICS DNA Miniprep Kit (Zymo Research, Irvine, CA). rDNA was amplified for Illumina sequencing between the V3 and V4 variable regions. A pool of all samples was analyzed on an Illumina MiSeq (Illumina, San Diego, CA) for a 300bp paired end run using the MiSeq v3 600 cycle kit.