Medical facility S1 no depletion
From March to December 2023, AerosolSense samplers were deployed in a cafeteria and medical facility in Northern NM, operating 4-8 hours daily over 5-18 days. Collected cartridges were processed by eluting biomaterial, concentrating with spin filters, and extracting total RNA using TRIzol. RNA was DNase-treated, cleaned, and rRNA was depleted in select samples using biotinylated probes generated from amplified 16S and 23S rRNA sequences. Libraries were prepared using a stranded template-switching protocol and sequenced on an Illumina iSeq 100 (150-cycle paired-end).