Single-cell RNA-seq of human T cells
T cells from a healthy donor (Donor_157) were in vitro stimulated with AR H875Y neoantigen and stained with Dextramer reagent. Double negative (Dextramer-) cells were sorted and subjected to single-cell RNA sequencing using the 10x Genomics Chromium Next GEM Single Cell 5' Reagent Kits v2 (Dual Index) according to the manufacturer's protocol