Metagenomic Data of the Microbiome in Diabetic Foot Ulcers (DFU)
The concentrations were quantified in a QUBIT (Thermo Fisher Scientific), and QIAxcel Connect (QIAGEN) capillary electrophoresis was employed for determining size distribution. These latter steps normalized libraries to 4 nM, followed by their pooling in readiness for sequencing on the Illumina NextSeq 550, set with High Output kit configuration in a paired-end mode with read lengths of 2x150bp.