16S rRNA sequencing of Mus musculus: fecal sample from 10-month-old male SAMP8 mouse
This study investigated the longitudinal dynamics of gut microbiota in SAMP8 mice at different ages (1, 3, 7, and 10 months) using 16S rRNA gene amplicon sequencing. Fecal samples were collected from male SAMP8 mice (n = 6 per age group). Genomic DNA was extracted and the V3V4 region of the 27S rRNA gene was amplified and sequenced using the Illumina MiSeq platform.