GSM8671036: Sample 1 WT-CD; Mus musculus; RNA-Seq
Nuclei were isolated from 50 mg fresh frozen cardiac samples (n=2) using the 10x Genomics Chromium Nuclei isolation kit (PN-1000494, with RNAse inhibitor; 10X Genomics, Pleasanton, CA, USA), conforming to the manufacturer protocol. scRNA-seq libraries were generated in BU DOM Single Cell sequencing core facility and sequenced by BU Microarray and Sequencing Resource Core Facility according to 10x Genomics protocol