GSM9363849: p60 Cnpy1-WT VNO biol rep 1; Mus musculus; RNA-Seq
Wildtype and Cnpy1-KO mice were euthanized using carbon dioxide asphyxiation, and the vomeronasal organ was collected. The outer bone was removed, and organs from both sides were used for RNA extraction in each biological replicate. Dissected tissue was transferred into tubes containing RLT buffer from the Qiagen RNeasy Plus Mini Kit with 1.4 mm ceramic beads. Tissue lysis was accomplished using a beadruptor, and subsequent steps were performed according to the manufacturer's instructions for the kit. A total of 500 ng of RNA was used for each library preparation using the NEB Ultra II Directional RNA-Seq Library Prep Kit (NEB Cat #E7760L) protocol. Poly(A) selection was performed using the NEBNext Poly(A) Magnetic Isolation Module (NEB Cat #E7490L), in accordance with the manufacturer's instructions. The libraries were indexed using NEBNext Multiplex Oligos for Illumina 96 Unique Dual Index Primer 96 rxn Set 3