GSM8776785: scRNA-seq_E14.5 HARE5Hs/Hs ,rep 2; Mus musculus; RNA-Seq
Two biological replicates from 2 different litters were used per genotype. E14.5 cortices were dissected in cold PBS, and dissociated using 0.25% Trypsin  (Thermo,69825200056) for 5 mins at 37°C ,cells finally resuspended in  PBS supplemented with 0.04% BSA. Cell viability and number were counted by a Cell Counter (Thermo). The scRNA seq experiment aimed to recover 8,000–10,000 cells from each embryo. cDNA was amplification and library construction were performed following 10x Genomics Chromium Single Cell 3' v3.1701 kit. cDNA libraries were quantified using the Agilent High Sensitivity DNA Kit (Agilent, 5067-702 4626) and were sequenced on an Illumina NovaSeq6000(PE150). Samples were sequenced to a depth of 40,000-70,000 reads per cell. A total number of 22,695 cells from two WT samples and 21,211 cells from two HARE5Hs/Hs sample were used for analysis, with an average of 2,110 genes detected per cell.