GSM9092928: scRNA-seq of renal cortex from T1DM mouse #4; Mus musculus; RNA-Seq
Renal cortex tissues were dissected, minced, and enzymatically dissociated using 150 U/mL collagenase II, 0.2% collagenase IV, 1.2 U/mL Dispase II, and 500 U/mL DNase I at 37°C for 45 minutes. The resulting single-cell suspension was filtered through 70 μm and 35 μm cell strainers and resuspended in PBS. Single-cell libraries were constructed using the Singleron GEXSCOPE® Single-Cell RNA Library Kit according to the manufacturer's instructions. Libraries were quantified, pooled, and sequenced using the Illumina HiSeq X Ten platform with 150 bp paired-end reads.