GSM9362890: 18_CLP+IgG_6h; Mus musculus; RNA-Seq
For RNA-sequencing library preparation, RNA was extracted from mouse lungs using RNA Plus Mini Kit (Qiagen) according to manufacturer's instructions. The concentration and integrity of total RNA was analysed using electrophoretic separation on microfabricated chips in Agilent 2100 Bioanalyzer System (Agilent, CA, USA). mRNA-sequencing library preparation (1.5 µg total RNA per sample) was carried out on NovaSeq 6000 PE150 instrument (2 x 150 bp, 40 million reads per sample).