GSM8843558: M2macrophage&Day28_1; Homo sapiens; RNA-Seq
TRIzol™ Reagent (Life Technologies, UK) was applied to extract total RNA from the contracts and then treated with chloroform. mRNA was isolated from the aqueous phase by sequentially adding isopropyl alcohol and 75% ethanol. RNA concentration and quality measurements were taken using NanoDrop. Library preparation, quality control and sequencing were performed by Novogene Co., Ltd. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina platforms, according to effective library concentration and data amount.