RNAseq of SafeCell AlloAccept tissue
Library preparation and Illumina sequencing were done at The University of Hong Kong, LKS Faculty of Medicine, Centre for PanorOmic Sciences (CPOS), Genomics Core. The libraries were denatured and diluted to optimal concentration. Illumina NovaSeq 6000 was used for Pair-End 151bp sequencing. Using software from Illumina (bcl2fastq), sequencing reads were assigned into individual samples with each sample having an average throughput of 157.2 Gb and a total throughput of 157.2 Gb. In terms of sequence quality, an average of 76% of the bases achieved a quality score of Q30 where Q30 denotes the accuracy of a base call to be 99.9%.  When sequencing under PE151bp configuration, average Q30 percentage of 10X 3 RNA libraries may be lower than Illumina standard due to sequencing into the polyT-region of the barcoded Gel Bead primers after cycle 28 of Read 1.