GSM9366781: wildtype Y. pseudotuberculosis replicate 3; Yersinia pseudotuberculosis; RNA-Seq
Supernatants were removed and pellets were resuspended in 1mL Qiagen RNA Protect Bacterial Reagent. Total RNA was isolated using the Qiagen RNeasy Mini kit and DNA was removed from samples using the Thermo Fisher TURBO DNA-free Kit. RNA quality was then assessed using an Agilent 2000 Bioanalyzer. Samples were DNAse treated with Invitrogen DNAse (RNAse free). Illumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus kit and 10bp unique dual indices