GSM8776787: Skin, vehicle, A2; Mus musculus; RNA-Seq
Mouse ear skin was harvested from AD-like model mice, dissected into small pieces, and digested with Liberase™ TL (0.25 mg/mL; Roche) at 37°C with gentle agitation. The digested tissues were filtered through a 70-μm cell strainer to generate a single-cell suspension. Live CD45⁺ Lin⁻ cells were sorted using a FACSAria cell sorter (BD Biosciences) for downstream scRNA-seq. A single-cell 3′ library was constructed using the 10x Genomics Chromium Single-Cell 3′ kit (v3). R1 (read 1 primer sequence) are added to the molecules during GEM incubation. P5, P7, a sample index, and R2 (read 2 primer sequence) are added during library construction via End Repair, A- tailing, Adaptor Ligation, and PCR. The final libraries contain the P5 and P7 primers used in Illumina bridge amplification.