GSM9037310: Control HGC-27 Rep 2; Homo sapiens; Bisulfite-Seq
Genomic DNA was extracted from HGC-27 cells following epigenetic silencing, as well as from control cells (those transfected with only gRNAs and untreated cells), using the Puregene Core Kit A (Qiagen) according to the manufacturer's protocol and the quality was assessed using Nanodrop. The amplified fragments with added tails were then gel-purified and their concentration was measured using Qubit™ dsDNA HS Assay kit (Invitrogen). Each amplicon was first diluted to a concentration of 15 nM, pooled in equimolar amounts per sample, and used for generating libraries with custom primers harboring unique dual indexes. The sequencing was conducted using NovaSeq PE150 for a minimum of 100,000 reads per amplicon.