GSM8843318: KO, Day3, replicate3; Mus musculus; RNA-Seq
mice were anesthetized with isoflurane followed by cervical dislocation post cryo injury day 3. The heart was rapidly excised, and the injured region was dissected and snap-frozen in liquid nitrogen. The tissue was homogenized in 1 ml of TRIzol (Invitrogen, 15596026) using ceramic beads with a BEAD RUPTOR 24 homogenizer (Omni, 19-070). The aqueous phase was collected, mixed with ethanol, and processed using the Qiagen RNeasy Mini Kit (Qiagen, 74134) according to the manufacturer's instructions." KAPA mRNA HyperPrep kit