Shotgun metagenome from Santa Monica Basin methane oxidizing enrichment
Approximately 15mL of the enrichment culture was collected and centrifuged for 30 minutes at 5000g and 10C. The supernatant was removed and the resulting cell pellet was extracted using the manufacturers Gram positive protocol with the Qiagen DNEasy Blood and Tissue Kit (Cat. #69504, Qiagen, Hilden, Germany). Whole genome library preparation and paired-ended sequencing was performed at the Millard and Muriel Jacobs Genetics and Genomics Laboratory, California Institute of Technology, using a P1 flowcell for 300 cycles.