GSM8669089: MACRO, RNA; Homo sapiens; RNA-Seq
For both Total RNA-seq and 3'mRNA-seq, Total RNA was extracted using the Zymo Direct-zol RNA Mini-Prep (50) with TRI Reagent kit (Zymo Research). RNA quality was determined by the RNA Integrity Number (RIN) using TapeStation RNA ScreenTape (Agilent). All retained RNA samples had a RIN > 8. 1 mg of total RNA was depleted of ribosomal RNA using the NEBNext rRNA depletion kit (New England Biolabs) for Total RNA-seq. rRNA-depleted RNA was purified with the miRNEasy kit (QIAGEN) and used to produce barcoded RNA sequencing libraries with the NEBNext Ultra Directional RNA Library Prep kit (New England Biolabs) for Total RNA-seq. For 3'mRNA-seq, sequencing libraries were prepared using the QuantSeq 3′-mRNA Seq Library Prep Kit for Illumina (Lexogen) from 500ng of total RNA.