Transcriptomic responses to diesel exhaust particles or World trade center dust
Transcriptomic responses to diesel exhaust particles or World trade center dust
20 mg of brain tissue was homogenized in TRIzol reagent using a BeadBug Benchtop Homogenizer. Brain tissue was suspended in 1 mL of TRIzol and homogenized for 6 rounds of 10 seconds homogenization and 60-second holds between each round. Following homogenization, 300 µL of chloroform was added, and samples were centrifuged with heavy gel phase-lock tubes (VWR, 10847-802) to separate the aqueous phase. RNA purification kit (Quantabio, Extracta Plus, Cat# 95214-050) was used following the manufacturer's protocol. RNA concentration and integrity were assessed with a Qubit 4.0 Fluorometer and Agilent Bioanalyzer prior to sequencing.  Library preparation (mRNA library, poly A enrichment) was performed by Novogene.