GSM8978438: LVCP, postnatal day 60, n=1; Mus musculus; RNA-Seq
Single-cell RNA sequencing was performed on lateral ventricle choroid plexus (CP) samples from mice at postnatal days P2-3, P11, and young adults (10 weeks), while single-cell multiome included P11 and young adult mice. CPs were isolated from perfused mouse brains, pooled (3–4 CPs/sample from both sexes), and enzymatically and mechanically dissociated through a multi-step protocol with pronase (0.1 mg/ml)  for 15min, Enzyme P mix (adult brain dissociation kit, 130-107-677, Miltenyi Biotec) for 30 min, and trypsin (0.025%) for 15min, with mechanic digestion every 5min. Cells pellets were resuspended in PBS-2%BSA. The Chromium Next GEM Single Cell 3ʹ v3.1 kit (10x Genomics) was used for scRNA-seq, following the manufacturer's guidelines. 10x Protocol for scRNA-seq