RNA-seq of fungus
Sequencing libraries were constructed from 3 g of total RNA. Poly(A)+ mRNA was purified using poly-T magnetic beads and fragmented. First-strand cDNA was synthesized with random primers and SuperScript II, followed by second-strand synthesis. After end repair, adenylation, and ligation of Illumina PE adapters, fragments of 400-500 bp were selected using AMPure XP beads. Library amplification was performed with Illumina PCR primers. Final library quality was assessed using an Agilent Bioanalyzer 2100.