GSM9416339: XHLT2-HS67-BLE1; Homo sapiens; RNA-Seq
Blood was collected in EDTA tubes within a 90 min window for all participants of the study. Peripheral blood mononuclear cells (PBMC) were obtained by density gradient centrifugation of whole blood at 800g for 30 min at room temperature without brake, using Ficoll-Paque (Cytiva). Isolated cells were cryopreserved in a solution containing fetal Bovine Serum and 10% DMSO (Sigma). Cell counts and viability of thawed PBMCs were determined using CellacaMX and 0.5 million cells were used for each sample. RNA was extracted using the Quick-RNA MagBead kit (Zymo Research) on the KingFisher automated extraction and purification system (ThermoFisher) according to manufacturer's instructions. Quality and quantification of extracted RNA was assessed on the 5300 Fragment Analyzer System (Agilent). The cDNA libraries were generated using the Universal Plus mRNA-Seq with NuQuant kit according to manufacturer's instructions (Tecan). The libraries were subsequently sequenced on a HiSeq 4000 platform (Illumina).