GSM8842058: mip-1∆, replicate 1 (RNAseq); Caenorhabditis elegans; RNA-Seq
Staged adult C. elegans (72 hours after L1 synchronization at 20°C) were collected, snap-frozen, and total RNA was extracted using TRIzol (Thermo Fisher cat # 15596026) and chloroform, using standard protocols. Total RNA was hybridized to anti-sense oligonucleotides (ASO) against C. elegans ribosomal RNA. Annealed ASO-rRNA hybrids were digested using Rnase H, as previously described (Duan et al. 2020). RNA sequencing libraries were prepared from 10ng of rRNA depleted RNA, from two independent biological replicates for each genotype, using the Illumina Stranded Total RNA kit (cat # 20040529) following the manufacturer's instructions and sequenced on a NovaSeq6000 system at the Johns Hopkins University School of Medicine Genetic Resources Core Facility.