GSM9416178: CAT, SSRP1-FKBP-E14, DMSO, TBP, clone1; Mus musculus; OTHER
Nuclei from 20,000M mESC and 6,000 S2 cells were extracted and lighlty fixed wit 0.1% FA. Nuclei were incubated with 5uL of activated ConA beads, 0.5uL primary Ab and 1:100 secondary Ab. Chromatin was then tagemented with 1:20 pAG-Tn5. Nuclei were washed to remove unbound pAG-Tn5  and then lysed with 5 μl SDS-ProK Buffer (1% SDS, 10 mM TAPS pH 8.5, Thermolabile Proteinase K).15uL 6% Triton was used to neutralise SDS. The tagmented DNA was amplified with NEBNext Ultra II Q5 Master Mix (M0544) and Nextera indexed primers. DNA was cleaned up with AMPure XP beads.