GSM9092492: pDC, WT, day12; Mus musculus; RNA-Seq
Bone marrow was isolated from femur and tibia. pDC, DC1 and DC2 populations were sorted by FACS. Cells from 5 (WT or KO) mice were pooled per sample. Cells were resuspended in RLT supplemented with 2-mercapthoethanol. Total RNA was isolated with the RNAeasy mini kit (Qiagen). cDNA libraries were generated using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara) as per manufacturers protocol.