GSM9039973: VV(2e6)-mono: 5%glucose ip, hIL-7/mIL-12-VV 2E6 pfu it, rep1; Mus musculus; RNA-Seq
Tumor tissues were harvested and quickly suspended in an RNA extraction solution (ISOGEN). Total RNA was isolated according to the manufacturer's instructions. Total RNA (>1 ug) was used as input for mRNA enrichment using a sequencing kit (SMART-Seq v4 Ultra Low Input RNA Kit). RNA libraries for RNA-seq were prepared using TruSeq stranded mRNA Library Prep Kit following manufacturer's protocols.