RNA-seq of P1/7RF
Ribosomal RNA was removed from the totalRNA and then precipitated with ethanol. After fragmentation, the first strand cDNA was synthesized using random hexamer primers. During the second strand cDNA synthesis, dUTPs were replaced with dTTPs in the reaction buffer. The directional library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.