Targeted sequencing of Fusarium oxysporum f. sp. lycopersici: tomato sample
For targeted enrichment, the following kits were used: Library Preparation EF 2.0 with Enzymatic Fragmentation, Twist Universal Adapter System, Twist Hybridization and Wash Kit with AMP Mix (part number: 106152), and Twist Custom Panel containing probes designed by Yu et al., 2023 (design ID: TE-93684965). Sequencing library was generated by Ligation Sequencing Kit (SQK-LSK110) provided by Oxford Nanopore Technologies (Oxford, UK). The sequencing libraries were then sequenced on a FLO-MIN106 (R9.4.1) flow cell using the MinION platform over a duration of 72 hours.