GSM9040685: Stressed calf 7; Bos taurus; RNA-Seq
Total RNA was extracted from filtered, washed, and pelleted bronchoalveolar lavage cells using the Qiagen Rneasy Mini Kit. RNA quality was evaluated using an Agilent 2100 bioanalyzer to verify minimum RNA integrity levels. Total RNA was poly-A enriched using magnetic beads (Novogene Corp.), and libraries were prepared using Novogene Corporation protocols