16S metabarcoding analysis of soil: ERF sample 48
Samples consisted of cores of topsoil (0-25 cm), sampled with a metal auger ( 3 cm). DNA was extracted following dx.doi.org/10.17504/protocols.io.4r3l29b9jv1y. Soil DNA was used to amplify the V4 region of the 16S ribosomal RNA gene, using primer pair 515bf-926r followed by paired-end 2 x 300 bp Illumina NextSeq sequencing.