GSM8908293: kidney, RP105-/- CLP1; Mus musculus; RNA-Seq
Total RNA was extracted from kidney tissue using TRIzol reagent (Invitrogen) following the manufacturer's protocol. RNA purity and integrity were assessed using NanoDrop and Agilent 2100 Bioanalyzer. Poly(A)+ RNA was enriched using oligo(dT) magnetic beads and fragmented. First- and second-strand cDNA synthesis was performed, followed by end repair, A-tailing, adapter ligation, and PCR amplification. Libraries were constructed using the NEBNext® Ultra™ RNA Library Prep Kit (NEB) and sequenced on an Illumina NovaSeq 6000 platform.