GSM9277881: H2AZac, NC 2, ChIP; Homo sapiens; ChIP-Seq
Crosslinked with 1% formaldehyde for 10 min at room temperature, quenched with 125 mM glycine, and chromatin was sheared to 100–600 bp using a Covaris M220 ultrasonicator. DNA was subjected to library preparation with NEBNext ChIP-seq Library Pre Reagent (NEB #E7370S, USA)