GSM8842064: N2, replicate 3 (smallRNAseq); Caenorhabditis elegans; ncRNA-Seq
Staged adult C. elegans (72 hours after L1 synchronization at 20°C) were collected, snap-frozen, and total RNA was extracted using TRIzol (Thermo Fisher cat # 15596026) and chloroform, using standard protocols. 5µg of total RNA was treated with 5' polyphosphatase. 1µg of purified, treated RNA was inputted into the Illumina TruSeq small RNA library prep kit (cat # RS-200-0012) according to manufacturer's instructions. Libraries were size selected, purified, and sequenced on a NovaSeq6000 system at the Johns Hopkins University School of Medicine Genetic Resources Core Facility.