WGS of Polish Konik horses: male whole blood
Libraries for WGS were constructed grouping 12 samples into four pooled sets to facilitate multiplex sequencing. Each prepared pool was subject to quality control, which included three main steps: concentration measurement (NanoDrop), size measurement (Bioanalyzer, Fragment Analyzer), and mimicking cluster creation (RT-PCR). The libraries were sequenced on the NovaSeq S4 Analyser (Illumina).