Homo sapiens
Firstly, the targeted loci were amplified using the target specific primers. In the second step, 33-nt adapters were attached to the 5 and 3 ends of the PCR amplicons from the first step through PCR amplification using target specific primers linked to the 33-nt adapters. In the third-round amplification, the PCR products from the second step were barcoded using Illumina index primer pairs. The amplicons were quantified using a Qubit 2.0 fluorometer then pooled the amplicons in equimolar ratio and prepared the libraries. The amplicon libraries were then subjected to paired-end sequencing on the MiSeq Illumina platform.