RNA-seq analysis performed on anthers isolated from Zhonghua 11 wild-type bio-replicate 3 (CRX1768787; CRA026580)
Genomic DNA or RNA is fragmented (200-500 bp), followed by end-repair and A-tailing using MGI-approved reagents. MGI-compatible duplex adapters (with sample-specific barcodes) are ligated to construct PCR-free or PCR-enriched libraries. Libraries are circularized, amplified into DNA nanoballs (DNBs), and loaded onto patterned flow cells for high-throughput sequencing.