DArT-seq for phylogeny analysis
We processed the DNA library with DARTSEQ to reduce complexity using a method that combines PstI-MseI (NEB) enzymes and replaces a single adaptor with two adaptors. Sequencing was performed by bulking equimolar amounts of amplification products from each 96-well microtiter plate sample and using them in a c-Bot bridge PCR system, followed by sequencing on the Illumina Hiseq 2500 platform (Illumina).