GSM9092936: Apc_KO_Kras_G12D_rep2_gex; Mus musculus; RNA-Seq
Nuclei from cryo-preserved intestinal tissue was isolated using the Nuclei Isolation Kit (10x Genomics, 1000494). Briefly, tissue was cut on ice in lysis buffer supplemented with RNase inhibitor, transferred to a 1.5ml tube, further dissociated using a pestle, and lysed for 10 min. The remainder of the nuclei isolation was performed according to manufacturing instructions. cDNA yield was quantified using High Sensitivity D5000 ScreenTape on a TapeStation (Agilent Technologies), library preparation was performed using Dual Index Plate TT (10x Genomics), followed by quantification on TapeStation (Agilent Technologies) and a Qubit fluorometer (Thermo Fisher Scientific). ATAC libraries were performed using Sample Index Plate N, Set A (10x Genomics, 3000427) by quantification on TapeStation (Agilent Technologies) and a Qubit fluorometer (Thermo Fisher Scientific) before submission for sequencing. The libraries were multiplexed and then sequenced on Illumina NovaSeq (Illumina) to generate at least 96M (expression) or 112M (ATAC) paired-end reads.