RNA-Seq of Nicotiana benthamiana: Pst.Dc3000 for 6 hour replicate 2
Sequencing libraries were prepared using poly(A) selection to enrich for mRNA. Total RNA was extracted, fragmented, and reverse-transcribed into cDNA. The cDNA libraries were then end-repaired, adapter-ligated, and PCR-amplified. Final libraries were quantified and quality-checked before paired-end sequencing (150 bp) on the Illumina NovaSeq X Plus platform.