GSM9087764: A549 RBM10wt, rep2; Homo sapiens; RNA-Seq
Total RNA was extracted from approximately 5×10e6 A549 cells using the QIAGEN RNeasy Mini Kit according to the manufacturer's instructions, including on-column DNase digestion. RNA was washed, eluted in nuclease-free water, quantified by NanoDrop, and stored at -80°C. Library prep and sequencing was performed at the Cincinnati Children's Hospital Medical Center DNA Sequencing and Genotyping Core. Between 150 to 300 ng of total was used for poly-A selection and library preparation with the TruSeq® Stranded mRNA Library Prep Kit (Illumina). Libraries were sequenced on an Illumina NovaSeq 6000, generating ~40 million stranded 100 bp paired end reads per sample.