GSM8618859: Col8a2Q455K/Q455K mice, control, rep3; Mus musculus; RNA-Seq
Total RNA was extracted from corneal endothelial cells using RNAlater and the RNeasy Micro Kit (QIAGEN) according to the manufacturer's protocol. RNA quantity and quality were assessed using an Agilent 2100 Bioanalyzer with RNA 6000 Pico Kit. RNA sequencing libraries were prepared using the SMART-Seq HT Kit (Takara Bio Inc.) following the manufacturer's protocol. After full-length cDNA synthesis, Illumina libraries were prepared using NexteraXT (Illumina Inc.).