GSM9337229: scRNAseq_E14_CTX_dKO_3000cells; Mus musculus; RNA-Seq
E14.5 or E17.5 littermate heads were harvested and stored in HibernateTM-E media (ThermoFisher, A1247601) supplemented with B-27™ (ThermoFisher, 17504044) (2%) and GlutaMAX™ (1%) (ThermoFisher, 35050061) for around 3 hours during genotyping. E14.5 cortices and E17.5 retinas from dHET and dKO littermates were then dissected into HibernateTM-E media before dissociation in Papain solution (NaOH (0,05%), Papain (10 units/mL), DNAse (0,004%), L-cysteine, in DPBS) during 2 to 4 min at 37ºC. Then mechanical dissociation was done adding Lo Ovomucoid solution and flushing cells in 1mL pipette tips. Eventually cells were filtered with a 60 μm filter and counted to resuspend them at 1000 cell/μL in PBS, 0,04% BSA. RNA library generation were generated using Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 Dual Index (10x Genomics, Pleasanton, CA)