GSM8705804: WT_HFD; Mus musculus; RNA-Seq
The liver samples were homogenized with a gentleMACS Dissociator (Miltenyi), filtered through 70 μm cell strainers, and then centrifuged at 400g through a Percoll density gradient (40% and 70%). The cells were loaded in the lanes using 10X Genomics Chromium Next GEM Single Cell 3' Kit v3.1 with a single capture lane per sample targeting recovery of 8,000 cells. All subsequent steps of library preparation and quality control were performed according to the 10X Genomics Chromium Single Cell 3' Reagent Kits User Guide.