Metabarcoding of fungal ITS: Boreal forest soils
DNA extracted with NuceloSpin Soil Kit (Macherey-Nagel). ITS2 amplified with gITS7 and 3:1 mix of ITS4 and ITS4arch. Purified with SeraMag magnetic carboxylate modified particles. Pools purified with E.Z.N.A cycle pure kit. Sequenced by SciLifeLab NGI with one SMRT cell per pool (five pools total).