GSM8668258: BMSC_Control_2; Mus musculus; RNA-Seq
Purification of RNA was performed with RNeasy Mini Columns (QIAGEN, Valencia, CA) with RNase-free DNase solution (QIAGEN) applied to degrade contaminating genomic DNA. RNA quantity was assessed with Nanodrop spectrophotometry (ThermoFisher). RNA libraries for RNA-seq were prepared using Illumina Total RNA Prep Kit following manufacturer's protocols.