GSM9039915: renal tissue, sham-2, Day14; Mus musculus; RNA-Seq
Total RNA was extracted using the MJzol Animal RNA Isolation Kit (Majorivd) following the standard operating procedures provided by the manufacturer. The RNA was then purified using the RNAClean XP Kit (Beckman Coulter) and RNase-Free DNase Set (QIAGEN). RNA integrity was assessed using the Agilent 2100 Bioanalyzer/Agilent 4200 TapeStation (Agilent technologies), and the total RNA quantity and purity were determined using the Qubit 2.0 Fluorometer (Thermo Fisher Scientific) and NanoDrop ND-2000 Spectrophotometer (Thermo Fisher Scientific). The purified Total RNA was subjected to mRNA isolation, fragmentation, first-strand cDNA synthesis, second-strand cDNA synthesis, end repair, 3' end A-tailing, adapter ligation, and enrichment to construct the mRNA sequencing library. The library concentration was detected using the Qubit 2.0 Fluorometer (Thermo Fisher Scientific), and the fragment distribution of the library was analyzed by the Agilent 4200 TapeStation (Agilent Technologies).