GSM8844307: WT in vivo organoid, biol rep 3; Mus musculus; RNA-Seq
Organoid and tumoroid lines were grown in ECM domes for 7 days. Lysis buffer (Qiagen KIT) was added directly to wells to collect the cells. Cells/buffer were vortexed for 10sec and incubated on ice for 5min before transferring to -80°C. RNA was extracted from all samples on the same day using Qiagen RNeasy Kit # 74104. 80ng/µl RNA was used for the construction of sequencing libraries. Libraries were constructed using Lexogen's QuantSeq 3' mRNA-Seq Library Prep Kit FWD V2. cDNA libraries were sequenced with an Illumina NextSeq 2000 platform