BES_t7
DNA was extracted from incubations using phenol-chloroform extraction and the DNeasy PowerLyzer PowerSoil kit. Duplicates were processed, and DNA was combined for further analysis. Negative and positive controls were included, with the positive control consisting of commercial microbial probes. The DNA was purified and concentrated using magnetic beads, then stored at -20C. For 16S rRNA gene analysis, amplicons were generated using universal primers (A519F and 785a-A21) and PCR with a touchdown protocol. Amplicons were purified and indexed using the Nextera XT Index Kit, followed by sequencing on an Illumina MiSeq V2 system (500 cycles, 250 bp paired-end). DNA concentrations were quantified and normalized before library submission to CeGaT GmbH for sequencing.