RNA-seq of cumulus cells from pig embryos without FLI supplementation that reached Blast stage
Cumulus cell mRNA was captured with oligo(dT) magnetic beads, fragmented, and converted into cDNA using random hexamers. Pre-index anchors were ligated and used for PCR amplification and cluster generation. The cDNA libraries were sequenced according to Illuminas standard protocol using the Novaseq X plus platform.