GSM8807963: Whole blood, SAINT-10_3, D7; Homo sapiens; RNA-Seq
RNA extraction from whole blood samples was performed using the Qiagen Blood miRNA Purification Kit, following the manufacturer's protocol. Whole blood was centrifuged to pellet the samples, washed with water, digested with proteinase K and homogenized via the provided PAXgene Shredder spin columns. Isopropanol was added to optimize binding conditions, and RNA was bound to the silica membrane in PAXgene RNA spin columns. After DNase digestion to remove genomic DNA, the RNA was washed and eluted with the provided buffer. Library preparation and sequencing were performed at Centre de Regulació Genòmica (CRG, Spain). Universal Plus mRNA-Seq with NuQuant, Human Globin AnyDeplete, UDI-C kit for Illumina was used to process the samples. RNA sequencing was performed with the Illumina NextSeq 2000 using single-end 50 bp reads and achieving an average sequencing depth of 40 million reads per sample.