cytb metabarcoding of Qiagen Powerwater sediment extraction blank: Laboratory control sample
Extracted DNA from reagents using the Qiagen Powersoil kit operating as negative controls were amplified with cytb metabarcoding primers (Snyder and Stepien et al. 2020) targeting fsh species with Nextera stye overhangs, and dual indexed with Nextera indices following the Illumina 16S metagenomics protocol (Illumina Part# 15044223 Rev B). Samples were 250 bp paired-end sequenced on an Illumina MiSeq v3.