GSM9089831: CER7-NT; Rattus norvegicus; RNA-Seq
Cell lysis and RNA extraction was conducted using the Norgen Single Cell RNA Isolation kit (SKU: 51800), by following the instructions provided with the kit for extraction, 48 hours following exposure. Following extraction, RNA concentration was determined using a Thermo Scientific Nanodrop instrument, and then samples were diluted to either 50 or 100 ng/uL depending on initial concentration. Resulting RNA isolates were kept at -80C until being sent off for further processing. Library construction (with polyA enrichment) and sequencing was conducted by Genome Quebec, with a requested read number of 25M 100bp-long reads per sample.