L4_lysogenized_by_Perkons_12-12-2024
Total DNA was extracted from an overnight culture obtained from a single colony of strain L4 lysogenized by phage Perkons (verified by PCR with Perkons-specific primers prior to sequencing) using Proteinase K/SDS treatment and Genomic DNA Clean & Concentrator-10 Kit (Zymo Research). Library preparation and the ligation sequencing of gDNA were carried out in accordance with the SQK-NBD114.24 kit protocol without any deliberate fragmentation or size selection; barcode #2 was used. The resultant library was sequenced using the Flongle (R10.4.1) flow cell in a Flongle adapter connected to MinION Mk1C. Fast basecalling model (dna_r10.4.1_e8.2_400bps_fast@v4.3.0) was used.