Shotgun sequencing
Shotgun sequencing approach using the NexteraXT DNA Library Preparation Kit from Illumina. In brief, an enzymatic reaction in this kit cuts DNA into fragments of approximately 300 bp, whilst simultaneously tagging these fragments with oligonucleotides, which are complementary to parts of the sequencing adapter and are added during the subsequent barcoding-PCR. All reactions were carried out following the instructions from the NexteraXT DNA Library Prep Reference Guide (Document #15031942 v01).