GSM8746557: control root 14d-2; Populus tremula x Populus alba; RNA-Seq
Total RNA was extracted from control root and mixed plant-fungal biomass (100 mg) using the RNeasy Plant Mini Kit (Qiagen). Biomass was ground in liquid nitrogen prior to extraction and an on column DNAse I treatment was included in the protocol. For samples containing poplar roots (EcM), extraction buffer RLC was supplemented with 2% PEG (polyethylene glycol) 8000. Preparation of libraries from total RNA (TruSeq Stranded mRNA Kit Illumina) and 2 x 150 bp Illumina HiSeq3000 sequencing was performed at the GET platform (Gé-nopole Toulouse Midi-Pyrénées, Auzeville, France) following their standard protocol.