gut viral metagenome A15
Qualifying samples were subjected to library construction using the ALFA-SEQ DNA Library Prep Kit, following its accompanying instructions. The main steps involved were: a) DNA fragmentation; b) End repair and 3' end A-tailing; c) Adaptor ligation, fragment selection, and purification; d) PCR amplification and purification. The size distribution of the constructed libraries was evaluated on the Qsep400 High-Throughput Nucleic Acid-Protein Analysis System, while the library concentration was measured using the Qubit 4.0. The amplified libraries were then sequenced using the PE150 protocol on either the Illumina platform.