GSM8958726: Rh41, adherent sample, biological replicate 3; Homo sapiens; RNA-Seq
Total RNA was extracted from adherent and tumorsphere-derived Rh30, Rh41, and RHB280217 samples using the RNeasy Mini Kit (Qiagen), followed by quality assessment with an Agilent 2100 Bioanalyzer RNA libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit and sequenced on an Illumina NovaSeq 6000 platform, generating 150-bp paired-end reads