GSM8746505: RNA-seq, mESC-Pou5F1-lin-del, Clone7, LIF; Mus musculus; RNA-Seq
Total RNA was isolated using RNeasy Mini Kit (Qiagen). RNA-seq libraries were prepared from 500 ng starting material and amplified for 8 cycles using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB E7490), NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB E7770) and NEBNext Multiplex Oligos for Illumina (NEB E7600).