GSM9416743: 2574278_CMY_Rep4; Homo sapiens; Hi-C
Hi-C libraries were generated following the in situ Hi-C protocol described by Rao et al. (2014). Briefly, 2 × 10⁷ cells were crosslinked with 1% formaldehyde, quenched with glycine, lysed, and chromatin was digested overnight with MboI restriction enzyme. DNA ends were filled in and labeled with biotin-dATP prior to proximity ligation. After proximity ligation, crosslinks were reversed and DNA was purified, sheared to 300–500 bp fragments, and biotinylated fragments were isolated by streptavidin pulldown. Sequencing libraries were prepared according to the Hi-C protocol and were compatible with Illumina sequencing platforms. Libraries were sequenced on an Illumina NovaSeq instrument using paired-end reads.