Genome of strain B35
After DNA quality control, the genomic DNA was fragmented to appropriate sizes, then SMRTbell cleanup beads were added to each tube of sheared DNA for purification. And then DNA fragments were damage repaired and end repaired. The SMRTbell library was produced by ligating hairpin-shaped sequencing adapters to both ends of the DNA fragments. After size collection using AMPure PB bead/gel cassette and AMPure PB beads purification steps, sequencing primer was annealed to the SMRTbell templates, followed by binding of the sequence polymerase to the annealed templates