RNA-Seq of DBV: clinical isolates from acute DBV patients in China
For deep sequencing library preparation, viral RNA extracted from patient serum was reverse transcribed using random hexamers. Following DNase treatment and purification, second-strand cDNA synthesis was performed. Libraries were prepared using Nextera XT reagents (Illumina), which involved fragmentation and adapter ligation in a single step through tagmentation process. The resulting libraries were purified and enriched by limited-cycle PCR before sequencing on the NovaSeq 6000 platform.