GSM8809664: efs_BS_rep1; Arabidopsis thaliana; Bisulfite-Seq
Genomic DNA was extracted from 10-day-old seedlings with Quick-DNA Plant/Seed Miniprep Kit (ZYMO, D6020) 200ng fragmented genomic DNA (200bp-500bp) was used for library preparation with VAHTS universal Pro DNA library prep kit for Illumina (Vazyme, ND608),NEBNext Multiplex Oligos for Illumina (NEB, E7535) were used for adapter ligation. After purification with VAHTS DNA Clean Beads (Vazyme N411), bisulfite conversion was performed with EZ DNA Methylation-Gold Kit (ZYMO, D5005), followed by DNA purification and PCR amplification