GSM9092440: PBS-sc; Mus musculus; RNA-Seq
Retinas from 6 mice per group were pooled together and cut into small pieces before incubated in Ames' Medium containing 15 U/mL papain (Worthington) and 0.1 mg/mL DNase I (Sigma) at 34 ℃.Cell pellets from NaIO3 group were resuspended, incubated with CD11b MicroBeads (STEMCELL Technologies), and magnetically separated using an EasySep™ Magnet (STEMCELL Technologies) according to the manufacture instruction for single cell RNA-sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols.