Shotgun metagenomics of the human fecal virome
Human fecal sample directly processed, extracting DNA and RNA using the AllPrep PowerViral DNA/RNA Kit. RNA was reverse transcribed, and both DNA and cDNA were amplified using SISPA. Library preparation was carried out using the DNA Prep (M) Tagmentation Kit and sequenced in an Illumina MiniSeq (PE150 reads).