GSM8978587: P023, CD4+IFN+ scRNAseq; Homo sapiens; RNA-Seq
IFNγ secreting cells were sorted by using IFNγ secretion-detection assay (Mitenyi) before cell sorting on MACSQuant Tyto (Miltenyi Biotec, Germany). IFNγ+CD4+ T cells were then encapsulated using the 10x Genomics Chromium Controller. Library was performed according to the manufacturer's instruction Chromium Next GEM Single cells 5′ Library Kit v2 ( 10x Genomics).