Shortgun Metagenomics of SynCom
Illumina sequencing libraries were prepared using the tagmentation-based and PCR-based Illumina DNA Prep kit, along with custom 10 bp unique dual indices (UDI) from IDT, targeting an insert size of 280 bp. Sequencing was performed on an Illumina NovaSeq X Plus sequencer in one or more multiplexed, shared-flow-cell runs, generating 2151 bp paired-end reads. Demultiplexing, quality control, and adapter trimming were conducted using bcl-convert (v4.2.4)