GSM9364639: 5_Parental_rep1_Nx_Pol2_pSer5_S5_combined; Homo sapiens; ChIP-Seq
Chromatin immunoprecipitation (ChIP) experiments were performed using anti-Rbp1 NTD (Cell Signaling, 14958), anti-Phospho-Rbp1 CTD Ser2 (Cell Signaling, 13499), or anti-Phospho-Rbp1 CTD Ser5 (Cell Signaling, 13523). In addition, 10ng of Drosophila melanogaster chromatin (Active Motif, 53083) and 1µg of Drosophila-specific histone variant H2Av antibody (Active Motif, 61686) were added to each reaction as a spike-in control for downstream normalisation. All libraries were prepared and sequenced by the US NCI CCR Genomics Core.