GSM9009971: HC-MuSC from patient SK236, Differentiation day 3; Homo sapiens; OTHER
Samples were processed with active motif CUT&RUN kit (Active motif, 53180) following manufacturer instructions with some modifications inspired from (Ding and Braun, 2023). 1 ug of anti-H3.3 antibody (Active motif, 91191) was used for CUT&RUN. DNA was purified by phenol-chloroform extraction before library preparation. Library preparation was performed as in Drmanac R, et al., Human genome sequencing using unchained base reads on self-assembling DNA nanoarrays. Science. 2010 Jan 1;327(5961):78-81. doi: 10.1126/science.1181498.