Microbial Communities in Rugao Ham
Using 20-50 ng of DNA as a template, amplification of the ITS1-ITS2 regions was performed using an upstream primer containing the sequence "GTGAATCATCGARTC" and a downstream primer containing the sequence "TCCTCCGCTTATTGAT". In addition, an adapter with an index was added to the end of the PCR product of 16S rDNA by PCR for NGS sequencing. The final PCR product library was detected by 1.5% agarose gel electrophoresis for the target fragment at 400bp.