GSM9151718: CUT&Tag of HU-HSA-3 using Kla Ab without glucose for 48 h; Canis lupus familiaris; OTHER
DNA from canine hemangiosarcoma cell line was digested by pAG-Tn5. For DNA amplification, PCR was performed for purified DNA fragments with index primers using PCR Master Mix. The process consists of 72°C for 5 min for 1 cycle, 98°C for 40 sec and 63°C for 10 sec for 13 cycles, and 72°C for 1 min for 1 cycle. Amplified DNA was purified with AMpure. DNA concentration was measured with Qubit using a dsDNA high sensitivity kit. DNA quality was checked with TapeStation using a D5000 High sensitivity kit.