Complete Chloroplast Genome of Diospyros blancoi from West Java, Indonesia
Total gDNA was used for the input of library preparation. The gDNA was fragmented using enzymatic methods to match the expected insert size . The fragmented DNA was ligated with Illumina-compatible Adapter. PCR was conducted to amplify the library and to generate the full-length adaptor containig unique index sequence in each library samples. The quality and quantity of library samples were determine using Qubit Fluorometer, Tape Station, and qPCR. The library samples were proceeded to sequencing with 300 cycle (150-PE) using Illumina NextSeq 500. The demultiplexing and Fastq generation was performed using Local Run Manager of NextSeq 500 Instrument.