GSM8843174: WT_STM_rep1; Homo sapiens; OTHER
Total RNA was extracted using the phenol:chloroform method. Ribosome-protected footprints were purified from monosome fractions by size selection and gel extraction. Disomes were similarly purified by size selection from the same samples. Following 3' dephosphorylation these fragments were subjected to adaptor ligation, RT, circularization and PCR amplification. Ribosome-protected footprints were purified from monosome and disome fractions by size selection and gel extraction. Following 3' dephosphorylation these fragments were subjected to adaptor ligation, RT, circularization and PCR amplification. The protocol is published in Sharma et al., 2021 (DOI: 10.1038/s41467-021-25411-y).