WGS of monoculture L. garvieae
Sample testing included concentration, sample integrity and purity. Concentration was detected byfluorometer or Microplate Reader (e.g. Qubit Fluorometer, Invitrogen). Sample integrity and purity weredetected by Agarose Gel Electrophoresis (Concentration of Agarose Gel:1% Voltage:150V, ElectrophoresisTime:40 min).1g genomic DNA was randomly fragmented by Covaris.The fragmented genomic DNA wereselected by Agencourt AMPure XP-Medium kit to anaverage size of 200-400bp.Fragments were endrepaired and then 3 adenylated. Adaptors were ligated to the ends of these 3 adenylated fragments.Thisprocess was to amplify fragments with adaptors from previous step. PCR products were purified by theAgencourt AMPure XP-Medium kit.The double stranded PCR products were heat denatured andcircularized by the splint oligo sequence. The single strand circle DNA (ssCir DNA) were formatted as thefinal library.Library was qualified by QC.The qualified libraries were sequenced : ssCir DNA moleculeformed a DNA nanoball (DNB) containing more than 300 copies through rolling-cycle replication. The DNBswere loaded into the patterned nanoarray by using high density DNA nanochip technology. Finally, pair-endreads were obtained by combinatorial Probe-Anchor Synthesis (cPAS)