GSM9010570: 11-Dex_hr_S35; Mus musculus; RNA-Seq
Cells were then lysed using RLT lysis buffer (Qiagen) and RNA was isolated from cells using RNeasy Mini Kits (Qiagen). RNA was quantified with Qubit (Thermo Fisher Scientific) and integrity was verified with TapeStation (Agilent Technologies). Ligation Kit with IDT for Illumina Unique Dual Index adapters following the manufacturer's recommendations except that half volume reactions were used. Libraries were pooled in equimolar proportions and quantified using the Invitrogen Collibri Quantification qPCR kit