GSM9363722: cGAS-KO; HD A3 (25172XR-01-12); Mus musculus; RNA-Seq
Three mice per group were used to generate biological triplicates (total n = 12). Striatal tissue was preserved in TRIzol reagent. RNA quality was assessed using High Sensitivity RNA TapeStation (Agilent Technologies Inc., CA, USA) and quantified using Infinite F Nano+ 200 Pro Tecan® (Tecan, Switzerland). Poly(A)+ RNA was isolated using paramagnetic beads coupled with oligo d(T)25 following the NEBNext® Poly(A) mRNA Magnetic Isolation Module manual. Samples were randomly primed (5´d(N6)3´ [N = A, C, G, T]) and fragmented before first-strand synthesis using Protoscript II Reverse Transcriptase (30 min at 42°C). All further steps followed the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®. Library concentrations were assessed using Qubit 2.0 (ThermoFisher), and quality was assessed with TapeStation HSD1000 ScreenTape (Agilent). Average final library size was ~400 bp, with ~250 bp insert size. Illumina® 8-nt dual indices were used.