GSM8810076: cpxS_noPT_60min_1; Pseudomonas aeruginosa PA14; RNA-Seq
Cells were collected by centrifugation (3220 x g, 10 min, 4 °C) and total RNA was isolated using a TRIzol lysis and the NucleoSpin miRNA kit (Macherey-Nagel). RNA was eluted in TE buffer and stored at -80 °C rRNA depletion (RiboZero Bacterial Kit), library preparation (TruSeq stranded total RNA) and sequencing (Illumina, HiSeq4000, 1 x 50 bp).