RNA-seq for oil content candidate gene analysis.
For Illumina RNA sequencing, sequencing libraries were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (#E7530L, NEB, USA) following the manufacturers recommendations. Then, the libraries were subjected to sequencing on an Illumina platform by Wuhan Benagen Technology Co., Ltd. (Wuhan, China), resulting in the generation of 150 bp paired-end reads.