RNA-seq of HCT-116
Briefly, the quantity and quality of RNA samples were assessed by Qubit 4.0 fluorometer (ThermoFisher Scientific) and Agilent 2100 Bioanalyzer (Agilent) respectively. cDNA libraries were prepared from RNA samples with Illumina TruSeq Stranded Total RNA Prep kit (ligation with Ribo-Zero Plus) and indexing kit (Illumina). The quantity and quality of the prepared cDNA libraries were assessed by Qubit 4.0 fluorometer and Agilent 2100 Bioanalyzer, respectively. The cDNA libraries of experimental and control groups were pooled, and the sequencing was done by an Illumina NextSeq 2000 system with the 150bp paired-end dual-indexed method.