GSM8776342: AT8C1, Normoxia, Rep4; Homo sapiens; RNA-Seq
The plates were incubated at 4ºC for 1 h and then washed four times with ice-cold PBS to give a pellet of neurospheres. RNA was isolated using a QIAshredder spin column (Qiagen). RNA was further isolated using AllPrep DNA/RNA Mini Kit (Qiagen). For library preparation, the Illumina Truseq stranded mRNA kit was used, and paired end read sequencing was performed on a NovaSeq 6000 machine