Ctr30d2 (CRX1747256; CRA026269)
16S rDNA was extracted from gut samples using the TIANamp Soil DNA Kit, and the concentration and quality of the samples were assessed using an Agilent 5400. After confirming the amplification quality of the DNA samples, PCR products were mixed and purified. The library construction was completed through steps including end repair, A-tailing, adapter ligation, and purification. After library quality was confirmed by Qubit and Q-PCR quantification, sequencing was performed on a NovaSeq6000 platform with PE250 sequencing.