cKp83
Genomic DNA was purified from overnight cultures by pelleting 1 mL of bacterial culture at 15,000  g for 15 min at 4 C. Samples were prepared according to the directions from the Wizard HMW DNA Extraction kit for Gram-negative Bacteria (Promega). The genomic DNA was rehydrated in DNA Rehydration Solution and Illumina sequenced (SeqCoast).