GSM8978177: morc-ATAC-1; Oryza sativa Japonica Group; ATAC-seq
Seedling were cut into small pieces and the nuclei were separated. 1× 105 nuclei were incubated with 50 µL transposition mix containing 2 µL Tn5 transposase (Vazyme, TD711) at 37 °C for 30 min. The mixture was further incubated at 55 °C for 30 min, and the fragmentation products were then subjected to DNA extraction using ATAC DNA Extract Beads.  fragments were sorted using ATAC DNA Clean Beads (Vazyme, TD711), and precipitated by cold ethanol. Purified genomic DNA was amplified using the universal i5 primer and barcoded i7 primer (Vazyme, TD202) using Vazyme Master Mix (Vazyme, TD711). The library PCR products were cleaned with Agencourt AMPure XP beads (Beckman Coulter, A63881).