sequencing of human metagenome
Genomic bacterial DNA was isolated from the collected tissues and swabs using the commercial QIAamp DNA Mini Kit (Qiagen, Germany). Stool DNA was isolated using the QIAamp Fast DNA Stool Mini Kit (Qiagen, Germany). Subsequently, the construction of bacterial 16S rRNA gene libraries was created using the Ion 16S Metagenomics Kit (Thermo Fisher Scientific, USA) and the Ion Plus Fragment Library Kit (Thermo Fisher Scientific, USA).