GSM8844258: KrasG12D+Twist1 overexpression Late,rep8; Mus musculus; RNA-Seq
Total RNA from each sample was extracted using TRIzol Reagent and qc was done through a NanoDrop (Thermo Fisher Scientific Inc.) and agarose gel (1%). To prepare the library, 1µg of total RNA was used. The libraries of cDNA were prepared using TruSeq sample preparation kit and enriched for mRNA with rRNA depletion and with ERCC spike-in