GSM8842442: spleen GD2TIF cells, 2 mon post transfer, scRNA; Mus musculus; RNA-Seq
Retro-virus targeting genes Bcor and Zc3h12a, and delivery GD2 CAR(14g2a) was spun infected into activated Cas9 transgenic T cells, and these T cells were transferred into immunocompetent C57BL/6 mice (1×10^6 per mouse) and distributed. 2 months after, 1×10^6 Splenic GD2TIF cells were sorted by an S3e sorter for library construction and scRNA-seq, TCR-seq. Single-cell suspensions (2×10^5 cells/mL) with PBS (HyClone) were loaded onto microwell chip using the Singleron Matrix® Single Cell Processing System. Barcoding Beads are subsequently collected from the microwell chip, followed by reverse transcription of the mRNA captured by the Barcoding Beads and to obtain cDNA, and PCR amplification. The amplified cDNA is then fragmented and ligated with sequencing adapters. The scRNA-seq libraries were constructed according to the protocol of the GEXSCOPE® Single Cell RNA Library Kits (Singleron).