GSM9151739: 129X1/Sv, Cerebellum_819; Mus musculus; Bisulfite-Seq
DNA was isolated from fresh mouse tissues (Liver and cerebellum) or from isolated cells (wight fat cells and colon epithelium cells). All tissues or cells incubated in lysis buffer (25 mM Tris-HCl (pH 8), 2 mM ethylenediaminetetraacetic acid, 0.2% sodium dodecyl sulfate, 200 mM NaCl) supplemented with 300 μg/mL proteinase K (Roche) followed by phenol:chloroform extraction and ethanol precipitation Bisulfite-seq reduced representation