GSM8745597: G4_blood neutrophils_with MSC treatment_S23; Mus musculus; miRNA-Seq
Cardiac tissues  were processed using the gentleMAC Octo Dissociator protocol, involving tissue dissociation, enzyme digestion, and centrifugation performed in single sessions under standardized conditions. Neutrophils were isolated  using anti-Ly6G microbeads and MACS column separation. Ly6G-positive cells were collected, centrifuged, resuspended in Trizol, and stored at -80°C. Total RNA was purified from samples using the PureLink RNA Mini Kit Details are provided in the materials and methods section