scRNA-Seq of mus musculus: adult male wt bown marrow
Single-cell suspensions of bone marrow cells isolated from male and female WT and VDR/ mice (6-8 weeks old) were prepared at a concentration of 1105 cells/mL in PBS and loaded to microfluidic chips (GEXSCOPE Single Cell RNA-seq Kit, Singleron Biotechnologies). ScRNA-seq libraries were constructed according to the manufacturers instructions (Singleron) and sequenced on the Illumina Novaseq 6000 platform with 150 bp paired-end reads.