RNA sequencing of Delphinium grandiflorum: flower
Total RNA was extracted using the Spectrum Plant Total RNA Kit (Sigma-Aldrich), followed by DNase I digestion using DNA-free DNA Removal Kit (ThermoFisher Scientific). Total RNA was assessed for quantity and integrity by Qubit (ThermoFisher Scientific) and RNA-nano assays (Agilent Bioanalyzer 2100), prior to whole transcriptome sequencing with an Illumina HiSeq 4000 (Novogene).