GSM9271012: HTPC_24h_control; Homo sapiens; RNA-Seq
HTPCs were derived from small testicular samples of one donor, with non-obstructive azoospermia and normal spermatogenesis, and were isolated using tissue-explant culture. In a concentration of ~1,100 cells/µl, 10,000 cells were processed using the Single-Cell 3' Reagent Kits v2 from 10xGenomics according to the manufacturer´s instructions. This was followed by GEM generation and barcoding, post-GEM-RT cleanup, cDNA amplification, library construction and quality assessment with the Bioanalyzer (Agilent).