GSM8881995: PBMC, healthy subjects Z5; Homo sapiens; RNA-Seq
RNA was extracted using a centrifugal column method. The E.Z.N.A.™ PX RNA Kit (omega biologics, USA) is designed for the isolation and purification of total RNA from blood samples in PAXgene whole blood RNA tubes. A Kaio K5500 Spectrophotometer (Kaio, Beijing, China) and an Agilent 2100 RNA Nano 6000 Assay Kit (Agilent Technologies, CA, USA) were used to check the purity, concentration and integrity of the RNA. Extract 3 ug of total RNA from each sample as the starting amount to construct strand specific libraries and small RNA libraries. Ribosomal RNA was removed from the samples using the Ribo-Zero™ Magnetic Kit. The constructed library was subjected to Illumina sequencing (NEB, USA).