GSM8881751: SLE_S1Pi_WB_20; Mus musculus; RNA-Seq
Individual tissue samples were lysed and homogenized in 850 µl of TRI Reagent (ThermoFisher Scientific) using the Tissue-Lyzer (Qiagen). Total RNA was isolated from the tissue lysate using MagMax 96 microarray kit (AM1839, Life Technologies) according to the manufacturer's instructions. RNA from blood and kidney from cenerimod- and vehicle-treated mice was isolated and prepared for sequencing using NUGEN Universal Plus mRNA kit with PolyA select (and globin depletion for the blood samples) according to protocol