MEL A1.3
We pooled flies across the two strain replicates for each of the eight strains, and snap froze them before extracting DNA using a modified phenol-chloroform-isopropanol method with RNAse A treatment. Mitochondrial genome sequencing using the Illumina Miseq 2x300 bp sequencing chemistry was performed at the Ramaciotti Centre for Genomics (University of New South Wales, Australia). We checked the raw reads for quality and trimmed the adaptor sequences before sequence analysis. A consensus library was constructed by merging forward and reverse reads and eliminating duplicates (from the raw FASTQ files) for each strain. We used a D. melanogaster mtDNA reference genome (Genbank accession number: NC_024511) to map the raw reads and extract sequences with gene annotations for each strain.