GSM9365173: Zebrafish retina – Control (biol rep 10); Danio rerio; RNA-Seq
Retinas were rapidly dissected under RNase-free conditions immediately after light exposure. Total RNA was extracted using the Qiagen RNeasy Mini Kit, following the manufacturer's instructions, including on-column DNase I digestion. RNA integrity was assessed with the Agilent Bioanalyzer, and only samples with RIN ≥ 7 were processed further. RNA-seq libraries were prepared using the Watchmaker RNA Library Prep Kit (Twist Bioscience). Poly(A)-selected mRNA was fragmented, reverse-transcribed, end-repaired, A-tailed, ligated to indexed adapters, and PCR-amplified according to the manufacturer's protocol. Libraries were quantified, pooled, and sequenced on the Illumina NovaSeq 6000 platform (paired-end 150 bp).