RNA-seq: microbial depletion in Exaiptasia pallida
R0 anemones were incubated in an antibiotic cocktail for one week. Total RNA was extracted from each anemone using the RNAqueous Total RNA Isolation Kit (Invitrogen). RNA quantity and integrity were quantified using a NanoDrop ND-1000 Spectrophotometer. Sample aliquots were normalized to a concentration of 10 ng/l submitted to the University of Texas at Austin Genomic Sequencing and Analysis Facility for TagSeq library development and sequencing on a NextSeq 1000.