Whole-genome sequencing of Homo sapiens: germline DNA from female siblings with familial breast cancer
Genomic DNA was fragmented to an average size of ~350 bp using Covaris sonication. Library preparation was performed using the Illumina TruSeq DNA PCR-Free protocol when input quantity allowed (>1 g), or the TruSeq Nano DNA Library Prep Kit when lower input DNA was available. End-repair, A-tailing, and adapter ligation were performed according to manufacturers instructions. Libraries were quality-checked, normalized, and pooled in equimolar concentrations for sequencing on the Illumina NovaSeq 6000 platform using paired-end 2150 bp reads.