Long read transcript analysis of D. sim F1 progeny of a cross between D. sim MD106ts x W501
Samples were flash frozen and freeze-dried. mRNA was isolated from approx. 15 heads using mRNA Direct Kit (Dynabeads) followed by full-length cDNA library construction using the Oxford Nanopore Kit SQK-PCB109 with barcoding. Barcoded samples were pooled and sequenced (ONT minion or promethion (UF ICBR). Fastq files were basecalled with Dorado (v2.1).