GSM8647932: Replicate 2 (E35), wild type AMsh; Caenorhabditis elegans; RNA-Seq
RNA was extracted from Trizol LS-treated cells by phase separation, following the manufacturers guidelines. RNA was purified using PicoPure RNA isolation kit. 0.4-5ng purified total RNA was obtained per sample. All subsequent steps were performed by the Rockefeller University Genomics Resource Center. RNA quality was verified using an Agilent Bioanalyzer to ensure sample degradation had not occurred. mRNA amplification and cDNA preparation were performed using the SMARTer mRNA amplification kit. Labelled samples were sequenced using an Illumina HiSeq 2000 sequencer and standard Illumina sequencing primers.