GSM8959990: WGBS_KD_2_28dpi; Procambarus virginalis; Bisulfite-Seq
Samples were digested using proteinase K (Invitrogen, #AM2546) and RNAse A (Sigma-Aldrich, #R6513). gDNA was precipitated using isopropanol and its quality assessed on a 2,200 TapeStation (Agilent). EZ DNA Methylation-Gold kit (Zymo Research, #D5006) was used for bisulfite conversion. Library amplification was performed using KAPA HIFI HotStart ReadyMix (Roche, #07958935001).