RNA-Seq of canine msc P6_Rep3
Next-generation sequencing (NGS) library preparation was carried out on 500 ng RNA for each sample, using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760) with NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490). Sequencing-ready libraries were quality control checked with a BioAnalyzer2100 instrument using High Sensitivity DNA Chip (Agilent Technologies USA, Cat. No. 5067-4626). NGS was carried out on the NextSeq 500 sequencing system with NextSeq 500/550 Mid Output Kit v2.5 (150 Cycles) chemistry (Illumina, Inc. USA, Cat. No. 20024904) targeting 20M reads/sample.