GSM9248253: exochew run3; Escherichia coli; OTHER
Cells were grown to sub-confluency in 10 cm plates, trypsinized, washed with PBS, and finally, lysed by adding 10 mM Tris (pH 7.4), 1 mM EDTA (pH 8), and 1% SDS. Chromosomal DNA was prepared using a Quick-DNA Midiprep Plus Kit (Zymogen; D4075). The hnRNP K pull down products were converted to dsDNA using an IDT xGEN ssDNA & LowInput DNA Library Prep Kit (Cat # 10009859) through the extension step according to manufacturer's instructions. The Oxford Nanopore Ligation Sequencing Kit (Cat # SQK-LSK114) was then used to ligate adapters and prepare the dsDNA, according to the manufacturer's instructions