GSM8881200: Viscoelastic, rep 2; Mus musculus; ATAC-seq
1,000,000 cells were added to ATAC lysis buffer after thawing and centrifuged at 500 g in a pre-chilled centrifuge for 5 minutes. Supernatant was removed and the nuclei were resuspended in 50 µL of tagmentation reaction mix by pipetting up and down. The reactions were incubated at 37 °C for 30 minutes in a thermomixer with shaking at 1,000 rpm, and then cleaned up using the MiniElute reaction clean up kit (Qiagen). Tagmented DNA was amplified with barcoded primers. Library quality and quantity were assessed with Qubit 2.0 DNA HS Assay (ThermoFisher), Tapestation High Sensitivity D1000 Assay (Agilent Technologies), and QuantStudio ® 5 System (Applied Biosystems). Equimolar pooling of libraries was performed based on QC values.