GSM8705451: ChIP-seq_H3_S10A_Mit_Repeat_2_with-spike-in; Homo sapiens; ChIP-Seq
For ChIP-seq, genomic DNA was isolated as per standard ChIP-seq protocol. For ATAC-seq, genomic DNA was isolated as per standard ATA-seq protocol, following viable nuclei isolation. For RNA-seq, total RNA was isolated using trizol as per the reagent manual. ChIP-seq and ATAC-seq library was prepared using the NEBNext® Ultra™II DNA Library Prep kit with Sample Purification Beads (Catalogue-E7103L) following the manufacturer's protocol. For total RNA-seq, rRNA depletion was done using NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat) with RNA Sample Purification Beads(Catalog no-E7405L) and library was prepared using NEBNext® Ultra™ II Directional RNA Library Prep with Sample Purification Beads(Catalog no-E7765L).