PC9C7-ENU-YC6 cell line RNA
RNA was extracted from 96-well plates as above. Stranded mRNA libraries were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) along with the IDT for Illumina Truseq RNA UD Indexes plate. Quantification was performed with the Qubit 1X dsDNA High Sensitivity (HS) Assay (Invitrogen) and Kapa Library Quantification Kit (Roche) prior to sample pooling. Libraries were sequenced at 2x151bp with dual indices.