GSM9271008: S12h_A; Danio rerio; RNA-Seq
Total RNA was extracted using Invitrogen TRizol reagent (California, USA). Generation of the Illumina RNA sequence library, along with paired end sequencing (300±50 bp) was conducted on an Illumina Hiseq 4000 (LC science, USA) using aliquots (5 mg) of qualified total RNA. Hisat software was used to align compare the sequencing data with the zebrafish reference genome (http://ftp.ensembl.org/pub/release-106/fasta/danio_rerio/dna/Danio_rerio.GRCz11.dna.toplevel.fa.gz). RNA libraries were prepared for sequencing using standard Illumina protocols.