GSM8668238: S11_K_MUT_V1_M; Mus musculus; RNA-Seq
Total RNA was extracted with Trizol protocol (15596018; Thermo Fisher Scientific, Waltham, MA). Samples were enriched for  PolyA RNA with the NEBNext® Poly(A) mRNA Magnetic Isolation Module(S7590S; New England Biolabs, Ipswitch, MA). 1ug of total RNA was used as input. All samples had RIN values > 8. Libraries for each sample were prepared following the protocol for the NEBNext Ultra II Directional RNA Library Prep Kit with Sample Purification Beads (E7765; New England Biolabs). cDNA libraries were then indexed using the NEBNext Dual Index Kit (E7600S; New England Biolabs).