GSM9364451: MULTI_CTRL_del TB_GEX; Mus musculus; RNA-Seq
2.5x104 pDCs from each genotype and stimulation condition were sorted on FACSAria II (BD Biosciences) at the Cytometry & Cell Sorting Laboratory at NYUGSoM and pooled together. pDCs = Live singlets CD11c+ MHC-II- B220+ Siglec-H+. Nuclei were isolated according to the Chromium Next GEM Single Cell Multiome ATAC + Gene Expression (GEX) protocol (10X Genomics) using an optimized 4 minutes of lysis. Libraries were prepared according to the 10X Genomics Single Cell Multiome ATAC Kit.