GSM8668959: 624Mel_siMITF+IFNg_3; Homo sapiens; RNA-Seq
Total RNA was extracted using a Quick-RNA Miniprep Kit acquired from Zymo Research. The standard protocol of the kit was followed with an additional washing step using 400 μL RNA wash buffer prior to the elution step. For cDNA library preparation, each RNA sample was diluted to 20 ng/μL in 50 μL final volumes. The cDNA libraries were then made using the TruSeq RNA v2 Sample Prep Kit (Illumina) and SuperScript IV Reverse Transcriptase (Invitrogen). The cDNA libraries were quantified using LabChip GX and diluted to 3 nM prior to sequencing. The sequencing was performed on a NovaSeq6000 sequencer, and the results were exported as FASTQ files using bclfastq2 v2.20.