DNA-SEQ reactor sample 4
The total DNA extracted from the samples was used for PCR amplification of the V4 variable region of the 16S rRNA gene from Bacteria and Archaea domains using specific primer pairs: 515F-806R for Bacteria and 341F-785R for Archaea. PCR products were purified using AMPure XP Beads and subsequently used to prepare the Illumina DNA library.