Iso-seq of DSE-kidney
Poly(A)+ mRNA cDNA was synthesized using the SMARTer cDNA synthesis kit (Clontech, USA), and full-length cDNA was then amplified through PCR employing PrimeSTAR GXL DNA polymerase (Takara, China). Subsequently, SMRTbell libraries were constructed using the SMRTbell Template Prep Kit 1.0 (PacBio, USA) according to the manufacturers instructions.