GSM8843291: RNA profiles of PANC-1 cells treated with Cannabichromene (CBC); Homo sapiens; RNA-Seq
Total RNA was isolated using Trizol reagent (Ambion, North Greenbush, NJ, USA). The isolation of mRNA was performed using the Poly(A) RNA Selection Kit (LEXOGEN, Inc., Austria), according to the manufacturer's instructions. RNA libraries were prepared from total RNA using the NEBNext Ultra II Directional RNA-Seq Kit (NEW ENGLAND BioLabs, Inc., UK), according to the standard Illumina protocols