GSM9410447: young, biol rep 1; Saccharomyces cerevisiae; RNA-Seq
Bulk RNA-seq was performed by Admera Health. Total RNA was extracted and then purified using RNeasy Plant Mini- Yeast (Qiagen Cat no. / ID.  74904). according to the manufacturer's protocols. Quality and quantity of RNA were assessed on a TapeStation (Agilent), and samples with an RNA integrity number (RIN) > 6 were used for subsequent library preparation. The Poly A selection + NEB Ultra II Directional RNA Library Prep Kit (New England BioLabs Inc., Massachusetts, USA) was used to generate cDNA and libraries according to the manufacturer's protocol. The libraries were indexed using Admera's in-house index primers and cleaned up using AFTMag NGS DNA Clean Beads (Abclonal science, RK20257). The indexed libraries were pooled and sequenced on the Illumina NovaSeq X plus platform with a read length of 2x150bp.