Shotgun metagenomics of the human fecal virome
Starting from human feces sample, viral particles were enriched using ultracentrifugation and treated with nucleases. RNA and DNA were co-extracted using the QIAamp Viral RNA Mini Kit. RNA was reverse transcribed, and amplification was performed using MDA. Libraries were prepared with the DNA Prep (M) Tagmentation Kit and sequenced in an Illumina MiniSeq (PE150 reads).