GSM8809963: MSC NC; Homo sapiens; ncRNA-Seq
RNA was isolated from 10µL of either control MSC EVs (no transfection), negative control miRNA transfected MSC EVs (NC MSC EVs) or let-7b-5p transfected MSC EVs (let-7b MSC EVs) using the miRNeasy Kit (Qiagen, Germantown, MD, Catalog #217004). In some cases RNA concentrations were too low to quantify, thus, the entire volume of extracted RNA was desiccated to 5ul. Qiaseq smRNA library preparation kit (Qiagen) was used for all samples and the workflow was performed according to manufacturer's instructions, using 24 cycles for final library PCR.