Dap-seg for TTC4-1 (CRX1735317; CRA025447)
First, genomic DNA was isolated from mature TW leaves, and a DNA library was constructed using the NEBNext DNA Library Prep Master Mix Set for Illumina (#E6040S/L; NEB, Beijing, China). Next, a Halo-tag in vitro expression plasmid of TTC4 was constructed, and the protein was produced using the TnT SP6 High-Yield Wheat Germ Protein Expression System (L3260; Promega, Beijing, China). DNA fragments that bound TTC4 were enriched by mixing the protein with the library and capturing them with magnetic beads.