GSM9414797: lung HIV infected replicate 6; Mus musculus; RNA-Seq
RNA extraction was performed using the Qiagen RNeasy mini kit (Cat. 74104), and the RNase-free DNase set (Cat. 79254). RNA quality was assessed with an Agilent Bioanalyzer, and RIN (RNA Integrity Number) values ranged between 9.8 and 10. PolyA+ RNA was purified from ~100 ng of total RNA. NEBNext Ultra II RNA library kit (New England Biolabs) was used to prepare the sequencing libraries following the manufacturer's protocol. Libraries (twenty-four) were quantified by qPCR, pooled, and sequenced using a single-end 75 base run on a Next-Seq 550 High-output flow cell at the UTMB Next Generation Sequencing Core.