Long- read sequencing of serum HBV RNA in HBeAg negative patient 19
PCR product was purified by 0.6x AMPure PB Beads and constructed the library by using the SMRTbell Express Template Prep Kit 2.0 (PacBio) with damage repaired, end repaired, A-tailing, and ligated the sequencing adapters according to manufacturer's instructions. The SMRTbell library was then purified by AMPure PB beads.