16S metagenome of Homo sapiens: Stool of adult male
DNA extraction from fecal and intraoperative samples from the GI tract was conducted using the ZymoBIOMICS DNA Miniprep Kit (Zymo Research Corp, Irvine, CA), following the manufacturer's protocol for isolation and purification. Initially, 50 mg of fecal matter underwent mechanical lysis using the MP Biomedicals FastPrep-24 5G Instrument (FisherScientific GmbH, Schwerte, Germany). Specifically, mechanical lysis was performed at 6 m/s for 45 s, repeated three times with 30 s intervals on ice between each lysis step. For profiling microbiome composition, the microbial DNA was sent to Novogene Company Limited (Cambridge, UK). To amplify the V3-V4 region of the 16S ribosomal RNA gene, polymerase chain reaction (PCR) with the following primers was used: CCTAYGGGRBGCASCAG, GGACTACNNGGGTATCTAAT.