GSM9150440: CAF#1, CUT&Tag (HSF1); Homo sapiens; OTHER
the cells are bound to Concanavalin A-coated magnetic beads, and the cell membrane is permeabilized by Digitonin. The enzyme pA-Tn5 Transposase precisely binds the DNA sequence near the target protein under the antibody guidance and results in factor-targeted tagmentation. DNA sequence is tagmented, with adapters added at the same time at both ends, which can be enriched by PCR to form the sequencing-ready libraries(CUT&Tag_Novogene Kit). After the PCR reaction, libraries were purified with the AMPurebeads and library quality was assessed on the Agilent Bioanalyzer 2100 system.