GSM8616898: Patient_2_rep1; Homo sapiens; RNA-Seq
High-viability, single-cell dissociation of fresh gastric tissue from three total gastrectomy specimens from three patients was collected for single cell sequencing utilizing the protocol detailed in Samaranayake, Sarah G., et al. "Protocol for isolation of signet ring cells from human gastric mucosa." STAR protocols 4.4 (2023): 102695. (https://doi.org/10.1016/j.xpro.2023.102695) The sample concentration and viability were assessed using the LunaFx7 fluorescent cell counter and diluted as necessary with PBS+0.04%BSA. The cellular samples were loaded in the lanes according to the 10X Genomics 3' v3.1 Single Cell User Guide. Cell partitioning completed successfully with uniform emulsion consistency and the reverse transcription PCR was run overnight. All subsequent steps of GEM recovery, cDNA generation, and quality control were performed as described in the 10X Genomics 3' v3.1 Single Cell User Guide. Libraries were prepared using the Chromium Connect.