miRNA-seq of human: control plasma
Plasma exosomal smaples were obtained by ultracentrifugation, then total RNA were extracted from exosomal and plasma samples and stored at -80C until library preparation. Individual samples were analyzed by random sequencing of small RNAs. Single patient libraries were prepared from each condition (total plasma or plasma exosomes) using Small RNA-Seq Library Prep Kit (Lexogen GmbH, Austria)  protocol to very low input samples.