GSM9366488: T_10; Homo sapiens; RNA-Seq
EVs were isolated by centrifugation at 20.000 xg during 70 min from frozen  citrate  plasma samples at -80°C after double centrifugation (10 min 2000 xg and 15 min 2500 xg) Plasma EVs were resuspended in 100 μL of lysis/binding Buffer (Invitrogen). RNA transcripts were captured with dynabeads oligo (dT) (Invitrogen) and reverse transcribed. RNA samples underwent a linear amplification by in vitro transcription followed by a fragmentation into 250–350 bp. Partial Illumina adaptor sequences were used, and a second reverse transcription reaction was performed. Full Illumina adaptor sequences were successfully added and the libraries sequenced (NextSeq 2000, Illumina)