GSM9248987: 24hpi VacV PKRIP-2; Homo sapiens; RNA-Seq
RNA for the RNAseq TRIzol and and the J2 RIPseq was extracted using TRIzol reagent, treated with DNase I, then purified with phenol:chloroform:isoamyl alcohol [25:24:1 (vol/vol)] and ethanol. RNA for the PKR fRIPseq was recovered with phenol:chloroform:isoamyl alcohol [25:24:1 (vol/vol)] followed by ethanol precipitation. Strand-specific RNA-sequencing libraries were prepared using the Ultra II Directional RNA Library Prep Kit (NEBNext) and submitted for paired-end sequencing on an Illumina NovaSeq 6000 with 150 cycles at Vanderbilt Technologies for Advanced Genomics (VANTAGE).