RNA-seq of Homo sapiens: HCT-116
RNA sequencing was performed on human colon cancer epithelial cells (HCT-116) treated with curcumin, three curcumin-based benzothiazepane analogues, and untreated controls. Each condition was performed in quintuplicates. Libraries were prepared using the QuantSeq 3 mRNA-Seq Library Prep Kit with UMI incorporation and sequenced as single-end 75 bp reads on an Aviti sequencer. The design allows comparison of transcriptomic responses between treated and control cells, and evaluation of selective cytotoxic effects of the compounds.