GSM8669689: CP139; Mus musculus; RNA-Seq
Total RNA was extracted from mouse SCN and GASTROC tissues using TRIzol reagent (Invitrogen, Carlsbad, CA) and purified by an RNA clean-up and concentration kit (Norgen Biotek, Thorold, ON, Canada). RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). RNA-seq libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina, per manufacturer instructions (New England Biolabs, Ipswich, MA). This pool was subjected to 151bp paired-end sequencing according to the manufacturer's protocol (Illumina NovaSeqXPlus). BCL Convert Conversion Software v4.0 (Illumina) was used to generate de-multiplexed Fastq files.