GSM8671060: P11; Homo sapiens; RNA-Seq
Total RNA extraction was performed using the RNe_x0002_asy Micro Kit (74004; Qiagen, Germantown, MD, USA) RNA reverse transcription was conducted using the MALBAC® Platinum Single Cell RNA Amplifica_x0002_tion Kit (KT110700796; Yikon Genomics, Suzhou, Jiangsu, China). 1 µl purified cDNA was reasonably diluted for detec_x0002_tion on the Agilent 2100 Bioanalyzer High Sensitivity DNA Chip (Agilent Technologies, Santa Clara, CA, USA). Library construction was accomplished using a gene sequencing and library preparation kit (XY045; Yikon Genomics, Suzhou, Jiangsu, China) according to the instruction manual. After purifcation, the libraries were quantifed using the Qubit dsDNA HS Assay Kit (Q32584; Invitrogen). Based on the results of Qubit quantitation, 10 ng of the library was taken for each sam_x0002_ple and mixed in equal proportion