16S amplicon metagenome of Philornis downsi: Gut
DNA was extracted from the gut homogenate using DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany) according to the manufacturers instructions. DNA samples were PCR-amplified with primers 515F and 806R targeting the V4 region of bacterial 16S rDNA, and sequencing was performed on a Illumina MiSeq platform.