KO-40D-1 (CRX1548853; CRA023605)
Testes were dissected in PBS and total RNA was isolated using TRIzol. mRNA was selectively enriched using Oligo(dT) beads post RNA extraction. Enriched mRNA underwent fragmentation into short fragments and was reverse transcribed into cDNA using the NEBNext Ultra RNA Library Prep Kit for Illumina.