GSM9040772: HBEC3KT, Pam2+ODN, 4h, Rep2; Homo sapiens; RNA-Seq
Cell were harvested after a 5–10-minute incubation with 1 mL of 0.25 % trypsin-EDTA, further neutralized with 4 mL of 2 % v/v fetal bovine serum in filter sterilized phosphate-buffered saline (PBS). Detached cells were centrifuged at 250 g for 5 minutes at room temperature (25 ºC) and the resulting cell pellet was reconstituted in 10 mL of GM. Cell counting was performed. A frozen pellet of 10,000,000 cells per sample was prepared and sent according to Active Motif for their end-to-end ChIP-seq service according to manufacturer's instructions. Chromatin preparation, sonication, sample quality/integrity assessment, ChIP and library generation, QC of NGS library, and sequencing using Illumina platform were performed using proprietary standard protocols and analysis by Active Motif.