GSM9248947: MPTP_BONT_A, scRNAseq; Mus musculus; RNA-Seq
The hippocampal tissue was dissociated using Papain. Cell suspension was then assessed for concentration, viability, and cell size using a TC20 Automated Cell Counter. Single cells were encapsulated with barcoded gel beads and partitioning oil to form GEMs (Gel Bead-in-Emulsions). After cell lysis, barcoded cDNA was synthesized, purified, and amplified. The cDNA was fragmented, end-repaired, and size-selected (~300 bp). Adaptors were ligated using T-A ligation, followed by PCR cleanup and validation before final library preparation.