Transcriptome sequencing-spleen
To construct and sequence the RNA libraries of 8 samples, total RNA was extracted from brain, heart, liver, spleen, lung, kidney, ovary, and pectoral muscle tissues. Use magnetic oligonucleotide (dT) beads to enrich Poly - A mRNA, fragment it, and reverse transcribe it into cDNA. Use the connector to prepare the library and perform double - end (2 x 125 bp) sequencing.