GSM8779199: A9_cKO_S1_rep1; Mus musculus; RNA-Seq
Hippocampal astrocytes were FACS-sorted from adult male GfapcreERT2:Tnfrsf1b^fl/fl and Tnfrsf1b^fl/fl mice. Cells were sorted based on ACSA-2 labeling using a BD FACSAria instrument. RNA was extracted from the sorted astrocytes using the SMART-Seq V4 Ultra Low Input RNA Kit (Clontech) following the manufacturer's protocol. cDNA was synthesized from the isolated RNA. Libraries were prepared (SMART-Seq V4 kit) and sequenced on an Illumina HiSeq 2500 platform to generate 125 bp paired-end reads.