16S V3V4 amplicon library from Blattella germanica ST-D3-1-2-TT
The V3V4 region of the 16S rRNA gene was amplified using primers 341F (CCTAYGGGRBGCASCAG) and 806R (GGACTACNNGGGTATCTAAT) with 3 Illumina TruSeq adapter tails. PCR products were purified with SPRI magnetic beads and subjected to a second PCR to add dual-index Illumina adapters. Libraries were pooled, purified, and sequenced using an Aviti 2300 sequencing cassette (Element Biosciences).