GSM9364094: HH.P1; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol reagent (Invitrogen) following the manufacturer's protocol.  RNA quantity and integrity were assessed using NanoDrop and Agilent Bioanalyzer. RNA-seq libraries were prepared using an Illumina TruSeq Stranded mRNA Library Prep Kit according to the manufacturer's protocol. Poly(A)+ mRNA was enriched, fragmented, and reverse-transcribed to cDNA, followed by end repair, A-tailing, adaptor ligation, and PCR amplification. Final libraries were quantified and assessed for quality before sequencing.