GSM8618582: E72,29190 ilium, scMultiomics,RNA; Homo sapiens; RNA-Seq
Fresh ilium tissues were extracted, digested using Collagenese type II, filtered and the cell count was calculated prior to moving on to following the 10X Genomics Multiomics protocol. cDNA was made, amplified and the DNA was purified using Ampure beads. Libraries were constructed following 10X Genomics multiomics manufactures protocols. Quality was checked using the tapestation, libraries were pooled and eventually sequenced using the NovaSeq X. These are sc-multiomics samples: a combination of scRNA+scATAC seq has been perfomed on the same set of cells.