GSM9012311: dMFB1 rep3; Saccharomyces cerevisiae; RNA-Seq
Total RNA was extracted using glass beads and phenol/chloroform/isoamyl alcohol (25:24:1). RNA was precipitated, and the quality and quantity of RNA was assessed with the Bioanalyzer. 1 mg RNA was used as input to the Illumina Stranded mRNA kit for the construction of sequencing libraries. RNA libraries were prepared using the Illumina Stranded mRNA prep kit following the manufacturers protocol.