GSM9416512: 2692159_2B10_H3K4me3_003; Homo sapiens; OTHER
Nuclei were isolated from intact cells and chromatin-bound proteins were selectively released using micrococcal nuclease (MNase) digestion following the Epicypher CUTANA CUT&RUN protocol. MNase digestion time was modified to 1.5 hours. CUT&RUN libraries were prepared using the CUTANA CUT&RUN Library Prep Kit (Epicypher; Cat#14-1001) according to the manufacturer's protocol following antibody-directed MNase digestion. Libraries were indexed, pooled, and sequenced on an Illumina NovaSeq platform.