GSM8777078: ATACseq_N2_15h_Rep1; Caenorhabditis elegans; ATAC-seq
Nuclei were prepared from frozen animal pellets by douncing similar to a previosuly established protocol (Daugherty et al., 2017, Genome Res.). Briefly, 7 rounds of douncing (3 times / round) were performed in Omni-ATAC buffer, and nuclei were seperated from worm debry by slow centrifugation. Isolated nuclei were then tagmented according to the Omni-ATAC protocol (Corces et al., 2017, Nat Methods), and DNA isolated using the MinElute PCR purification kit (28004, Qiagen). Isolated DNA was amplified using NEB Next High-Fidelity PCR Master Mix (M0541S, NEB), and barcoded illumina sequencing primers before Ampure XP bead purification.