RNA-seq of D. Citri collected from northern Upolu
A total of 150 adult D. citri psyllids collected in 2022 from the two main islands of Samoa were divided into three groups for RNA-Seq analysis, allowing the workload to be balanced and kept within budget constraints. One group consisted of samples from Savai'i, while the other two groups comprised samples collected from the northern and southern regions of Upolu. Adult insects were transferred into QIAzol lysis reagent, and total RNA was extracted following the manufacturers protocol (QIAGEN; Cat No.: 79306). The RNA samples were treated with DNase I for 1 hour at 37C. Concentrations were measured using a spectrophotometer, and integrity was confirmed by electrophoresis on a 1% (w/v) agarose gel. After verifying RNA quality, total RNA from three pooled groupsSavai'i (SAV), northern Upolu (UPO1), and southern Upolu (UPO2)was submitted to the Novogene sequencing facility in Hong Kong for library preparation (following eukaryotic ribosomal RNA depletion) and strand-specific total RNA sequencing on the NovaSeq platform. The eukaryote ribosomal RNA was removed using a VAHTS Total RNA-seq (HMR) Library Prep Kit for Illumina.