GSM8978188: ZH11-HiChIP-6B-2; Oryza sativa Japonica Group; OTHER
Briefly, nuclei were isolated from rice leaves (14-day-old), digested with Dpn II (New England Biolabs, R0543T), immuno-precipitated with the anti-H3K27me3 and anti-OsMORC6b antibody. The precipitated chromatin DNA fragments were recovered for constructing Hi-ChIP-seq libraries. The beads were directly subjected to Vazyme TruePrep DNA Library Prep Kit (TD501) for library construction.