GSM8844423: U2OS, siORCA, replicate_1, NS_seq; Homo sapiens; OTHER
Cells were fixed with 1% formaldeyde for 10 mins at room temperature. After quenching and cell lysis, chromatin was sonnicated using Bioruptor Power-up (Diagenode) For NS-seq DNA from cell cultures was isolated, size fractionated and subject to exunuclease digestion as described in Aladjem et al., Science 281, 1005, 1998. and Wang et al., Molecular and Cellular Biology 24:3373-86, 2004.