RNA-seq of Prunus sibirica of NF:sample1
Transcriptome sequencing was performed of P. sibirica at six developmental stages of early and late flowering. The RNA concentration was measured with high precision using a Qubit 4.0 fluorometer/MD microplate reader. The integrity of RNA was accurately detected by a Qsep400 bioanalyzer. Subsequently, cDNA chains were synthesized through reverse transcription, an A tail was added, and sequencing adapters were ligated. Finally, PCR enrichment was conducted to obtain the ultimate cDNA library. After the library construction, the quality of the library was inspected. Sequencing on the machine could be performed only when the inspection results met the requirements. The detection methods were as follows: initial quantification was carried out using the Qubit dye method, the insert size of the library was detected using a fragment analyzer, and the next experiment could be conducted only when the insert size met expectations; the effective concentration of the library was accurately quantified using the Q-PCR method (the effective concentration of the library > 2 nM), and the library inspection was completed. After the library inspection was qualified, different libraries were pooled according to the target amount of downstream data and sequenced using the Illumina platform.