Bulk RNA-seq Comparison of Populations of Intestinal Tregs
Bulk RNA-seq Comparison of Populations of Intestinal Tregs
Samples were sorted into Trizol reagent, frozen at -80, and sent to Genewiz/Azenta Life Sciences for Ultra-low input RNA-seq. Total RNA was extracted from cells following the Trizol Reagent User Guide (Thermo Fisher Scientific). Extracted RNA samples were quantified using Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA) and RNA integrity was checked using Agilent TapeStation 4200 (Agilent Technologies, Palo Alto, CA, USA). The SMARTSeq HT Ultra Low Input Kit was used for full-length cDNA synthesis and amplification (Clontech, Mountain View, CA), and Illumina Nextera XT library was used for sequencing library preparation.