Illumina NovaSeq 6000 paired Sequencing of ALE Chlorella (KAS24172548) (KAE24107414)
The qualified genomic DNA sample was randomly fragmented by Covaris technology and the size of the library fragments was mainly distributed between 150bp and 250bp. The end repair of DNA fragments was performed and an &quot;A&quot; base was added at the 3&#39;-end of each strand. Adapters were then ligated to both ends of the end repaired/dA tailed DNA fragments for amplification and sequencing. DNA libraries were prepared using the TruSeq DNA PCR-Free Sample Preparation Kit (Illumina, USA) according to the manufacturer&rsquo;s protocol. The TruSeq Rapid PE Cluster Kit (Illumina, USA) was used for cluster generation, and sequencing was carried out on an Illumina platform using the TruSeq Rapid SBS Kit (Illumina, USA). High-throughput paired-end sequencing was performed to ensure that each sample met the desired average sequencing coverage. Raw image files were processed using Illumina&rsquo;s base-calling software with default parameters, and the resulting paired-end reads were stored in FASTQ format as raw data.