GSM9149747: NC_IP; Homo sapiens; RIP-Seq
RNAs that bind to SRSF11 were identified with RIP kit (BersinBio, Guangzhou, China). In brief, polysome lysis buffer mixed with protease inhibitors and RNase inhibitors was used to lyse the cells on ice. After the DNA was removed, the anti-SRSF11 antibody or anti-Flag antibody and an equal amount of anti-IgG antibody were added to the lysis mixture, which was subsequently incubated at 4°C for 16 h. Then, protein A/G beads were used to collect immunoprecipitates. Finally, TRIzol was used to extract RNA from immunoprecipitated samples and input samples. High-quality RNA was broken into short fragments via fragmentation buffer, and a library was constructed via the KCTM Digital Stranded mRNA Library Prep Kit (Seqhealth Technology. Co., Ltd., Wuhan, China).