Drosophila embryos, granule fraction, biological rep 2
The concentration of each extracted DNA fragment was determined using Qubit 4 fluorometer with 1X dsDNA HS assay kit (Thermo Fisher: Q32854). For each library preparation, 100 ng of pooled DNA fragments were used as input and prepared using the NEBNext Ultra II DNA library prep kit for Illumina (NEB: E7645S) and its protocol. The prepared DNA libraries were sent for sequencing using the MiSeq system by the Johns Hopkins Genetic Core Facility.