WGS of Culex pipiens complex: whole body
Genomic DNA was extracted using the NucleoSpin 96 DNA RapidLyse kit (Macherey-Nagel, Germany). DNA sequencing libraries were prepared using Illumina DNA Prep Kits (Illumina, USA) with custom dual-unique barcodes. Approximately 80 barcoded libraries were pooled and sequenced on individual S4 lanes of a Novaseq 6000 PE150 sequencer (Illumina, USA), with a target genome-wide coverage of 10-15X.