GSM9278097: LX-2 cells,con1 BS; Homo sapiens; Bisulfite-Seq
Total RNA was harvested using TRIzol reagent(Invitrogen, cat. NO 15596026）, and a minimum of 2 μg of total RNA per sample was used for the construction of sequencing libraries . PolyA+ RNA was then selected and the library was constructed using the EZ RNA Methylation™ Kit (ZYMO RESEARCH) and KC UMI RNA Library Kit (Wuhan SeqHealth Tech) according to the manufacturer's instructions.