GSM8778860: ko24_1; Mus musculus; RNA-Seq
RNA was extracted from activated or non-activated CD4T cells (human or mouse) with the RNAqueous Micro Kit (Invitrogen, # AM1931,) followed by DNase digestion (TURBO DNA-free™ Kit, # AM1907) RNA was quantified and applied to the TapeStation (Agilen) for quality assessment. RNA was subjected to library preparation using poly(A) mRNA capture (NEB, # E7770 and #E7490) according to manufacturer's protocol