GSM9011624: Serum_Positiv_Endo_16; Homo sapiens; miRNA-Seq
The miRNA was extracted from each serum and urine sample using the miRNeasy Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. Sequencing libraries were prepared with the QIASeq miRNA UDI Library Kit (Qiagen, Hilde, Germany) according to the manufacturer's instructions. To the recommended 4 µl sample input for biofluids, 1 µl of synthetic miRNAs from the QIASeq miRNA Library QC Kit was added as additional quality control. The quality of the libraries was checked on a Bioanalyzer or Tapestation (both Agilent, Waldbronn, Germany), and the libraries were quantified by a Quantus fluorometer (Promega, Madison, WI, USA).