RNA-seq of Trichogramma dendrolimi:adult female
mRNA was purified from the total RNA using oligo (dT) magnetic beads and fragmented into 300350 bp fragments. First-strand cDNA was synthesized using random hexamer primers, followed by second-strand cDNA synthesis using DNA polymerase I and RNase H. The resulting double-stranded cDNA was subjected to end repair, phosphorylation, and ligation with Illumina paired-end sequencing adapters. The libraries were then enriched by PCR amplification and purified with an Illumina NovaSeq X plus platform according to the manufacturers protocol.