GSM9413815: BC-TuX-4IC-MFAP2_OE_replcate 2; Homo sapiens; RNA-Seq
Total RNA was extracted from the cell lines using Quick RNA Miniprep Kit from Zymo Research (Irvine, CA, USA) and sent to Azenta (TU-BcX-4IC) (Burlington, MA, USA)  for RNA sequencing. The RNA sequencing workflow after RNA isolation included initial PolyA selection: based mRNA enrichment, mRNA fragmentation, and random priming with subsequent first- and second-strand complementary DMA (cDNA) synthesis. Afterward, end-repair 5' phosphorylation and nucleotide (dA)-tailing were performed. Last, adaptor ligation, polymerase chain reaction (PCR), and Illumina NovaSeq technology-based sequencing with 2 150-base pair (bp) read length were carried out.