GSM9361351: Uninjured, P3, Rep2; Mus musculus; RNA-Seq
Digit amputations were performed on 6-8-week-old mice as described previously.Briefly, mice were orally administered meloxicam for analgesia and anesthetized with 5% isoflurane immediately before digit tip amputation surgeries. Then, using sterile scalpels, the distal one-third of terminal phalanges 2, 3, and 4 were amputated to induce a regenerative response. For non-regenerative amputations, the distal one-third of the second phalanx was amputated. Cells were dissociated with...and were pooled from N=3-5 unique mice, centrifuged and filtered with a succession of 40µm and 30µm filter. Cell concentration was determined and resuspended to 372 cells/µL in 2% FBS in PBS. Libraries were prepared by using Chromium Next GEM Single cell 3'mRNA v3.1 as per the company's protocol, and sequenced on NovaSeq 6000