RNA-seq: microbial depletion in Exaiptasia pallida
R7 anemones were incubated in an antibiotic cocktail for one week followed by one week in filter-sterilized artificial seawater. Total RNA was extracted from each anemone using the RNAqueous Total RNA Isolation Kit (Invitrogen). RNA quantity and integrity were quantified using a NanoDrop ND-1000 Spectrophotometer. Sample aliquots were normalized to a concentration of 10 ng/l submitted to the University of Texas at Austin Genomic Sequencing and Analysis Facility for TagSeq library development and sequencing on a NextSeq 1000.