GSM9151181: wildtype replicate 2, sample 15; Escherichia coli K-12; RNA-Seq
Total RNA was extracted from 1.5 ml of culture using RNAprotect Bacteria Reagent (Qiagen, cat. No 76506) and RNeasy Mini Kit (Qiagen, cat. No 74134) with on column DNase I digestion (Qiagen, cat. No 79254), according to the manufacturer's instructions (Qiagen, Germany). Illumina TruSeq RNA Library Kit (with ribo-depletion, without Poly-A selection)