GSM8808929: BeWo cells, TMEM63B KO2; Homo sapiens; RNA-Seq
Total RNA was extracted using a Trizol reagent kit (Vazyme) according to the manufacturer's protocol. 1.5 μg of total RNA was used for the construction of sequencing libraries. After total RNA was extracted, eukaryotic mRNA was enriched by Oligo(dT) beads. Then the enriched mRNA was fragmented into short fragments using fragmentation buffer and reversly transcribed into cDNA by using NEBNext Ultra RNA Library Prep Kit for Illumina(NEB#7530, New England Biolabs, Ipswich, MA, USA). The purified double-stranded cDNA fragments were end-repaired, A base added and ligated to Illumina sequencing adapters. The ligation reaction was purified with the AMPure XP Beads(1.0X). And polymerase chain reaction (PCR) amplified.