Ast-33 (CRX1732539; CRA025957)
Design specific primers for the 16S V3-V4 region to amplify a target area, resulting in an approximately 420bp amplification fragment. Add adapters, and use the Illumina Novaseq6000 platform for sequencing to obtain 2x250bp paired-end data. Through assembly, longer sequences can be obtained for subsequent 16S analysis.