Serial passaging of ANDV: CHI-7913 P5
A tiling amplicon scheme was designed using PrimalScheme. The primers were pooled into 3 pools and used for RT-PCR using SuperScript VILO and Phusion U Multiplex Master Mix. The amplicons from each pool were purified and combined (within samples) at equivalent masses. Libraries were prepared using a Nextera DNA Flex kit and sequenced on an iSeq 100.