GSM8618254: Kidney Pre-treatment Biopsy, NAXIVA Responder, Patient 17; Homo sapiens; RNA-Seq
RNA was extracted from formalin-fixed paraffin-embedded (FFPE) tissue using the ReliaPrep FFPE Total RNA kit, according to manufacturer's instructions, and assessed by Qubit and Agilent RNA ScreenTape System and for quantity and quality. RNA library preparation was done using the Watchmaker Genomics RNA Library Prep Kit with Polaris Depletion, according to manufacturer's instructions and running 18X PCR cycles for each sample. Indexing was done using the xGen™ Stubby Adaptor and UDI primers from Integrated DNA Technologies™, and sequencing via Illumina sequencing. The samples were run on an S4 flow cell on NovaSeq6000 with a read length of PE50. Manufacturer's instructions followed for the run, including spike-in of 1% PhiX.