GSM8911758: Nerve cells co-cultured with Vegf siRNA transfected IVD cells, sample 3; Homo sapiens; RNA-Seq
Post co-culture experiments, HMEC-1 or SH-SY5Y cells were lysed with TRIzol reagent and the total RNA was extracted with a Direct-zol RNA MicroPrep Kit. Library preparation was performed with 10ng of total RNA with a Bioanalyzer RIN score greater than 8.0. ds-cDNA was prepared using the SMARTer Ultra Low RNA kit for Illumina Sequencing (Takara-Clontech) per manufacturer's protocol