GSM8810059: N4, Normal, hepatocellular carcinoma, scTCR-seq; Homo sapiens; RNA-Seq
The cells are washed and resuspended to prepare a suitable cell concentration of 700~1200 cells/ul for 10x Genomics ChromiumTM. For single-cell isolation, Gel Beads in Emulsion (GEMs) were constructed based on the target cell count. Post oil treatment, the amplified cDNA was purified using magnetic beads, followed by cDNA amplification and quality assessment. The single-cell suspension was loaded onto a microfluidic device to construct a single-cell 3' gene expression library. The final library was sequenced on the Illumina Nova6000 platform using 150 bp paired-end reads.