GSM8843384: WT polysome RNA rep1; Mus musculus; RNA-Seq
the mRNA of whole cell lysate and the mRNA of polysome and monosome fraction after polysome profiling were harvested using Rneasy mini plus kit (Qlagen). mRNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.