GSM8809883: sorted macrophages,NE stimulation,rep2; Mus musculus; RNA-Seq
Then, the RNA from the sorted cells was extracted using the RNeasy Plus Micro Kit as per manufacturer instructions. Poly(A)mRNA was isolated using mRNA Capture Beads2.0 (Yeasen Cat.12629ES,CHN) with two rounds of purification, followed by RNA fragmentation with magnesium ions at 94˚C (Yeasen Cat.12340ES97,CHN) .RNA sequencing library preparation was performed using the TruSeq RNA Library Prep Kit v2 (Illumina).