GSM8978134: mm202C-sgBrpf1-3_RNA_steadystate_REP1_250408_ACTTCTGC_S1; Mus musculus; RNA-Seq
For RNA isolation from cell lines, 1x10^6 cells were lysed in 350 µl of RLT buffer and RNA was isolated using the RNeasy Mini Kit (Qiagen) according to manufacturer's instructions for cell line experiments. 1.2 ug RNA was used as the input quantity for library construction. Poly-A tail selection and library preparation were performed using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA).