GSM8775797: 46301, C118F, DMSO; Mus musculus; RNA-Seq
Both kidneys together with neighboring stretches of the abdominal aorta were removed and perfused with suspensions of tosylactivated magnetic dynabeads® in 1x HBSS pH 7.5. Podocytes were isolated. After the final digest, cell suspensions were passed through a 40 μm cell strainer and subjected to FACS.  Podocytes were collected in RNAlater® and then processed using the RNeasy Plus Micro kit (Qiagen). Library preparation was carried out as described in the SMART-Seq-mRNA LP User Manual (Takara Bio, Inc., CA, USA)