GSM8777815: CSC9_0701086501; Homo sapiens; RNA-Seq
RNA extraction and purification from CICs and FBS cell lines were performed using the AllPrep DNA/RNA Mini Kit  The amount and the quality of nucleic acids have been assessed by the Nanodrop 8000 and Qubit PicoGreen assay and RNA RIN threshold has been determined by the 2100 BioAnalyzer. Total RNA was used for library preparation using the Illumina Truseq Stranded Total RNA HMR kit which assists in depleting ribosomal RNA (rRNA) using biotinylated oligos combined with streptavidin-coated magnetic beads.