GSM8956168: Bromocriptine-feded forager bees; Apis mellifera; RNA-Seq
Honeybee were anesthetized in a dissection dish on ice and washed with ethanol and phosphate buffer saline (PBS) twice, after which brains were dissected in PBS on ice under a stereomicroscope (OLYMPUS EP50). The surrounding trachea, ocelli, and compound eye (absent in drones) were carefully removed, after which honeybee brains were washed with 1ml PBS. The single nuclei were prepared by mechanical extraction. snRNA-seq libraries were constructed using Single Cell 3' Library and Gel Bead Kit V3.1. The libraries were finally sequenced using an Illumina Novaseq 6000 sequencer with a sequencing depth of at least 100,000 reads per cell with pair-end 150 bp (PE150) strategy.