GSM8666823: mid-blastula embryos, derived from sperm_0day-post stripping, rep2; Cyprinus carpio; RNA-Seq
Total RNA was extracted from embryos using RNA isolation kits (Invitrogen, USA) in accordance with the instructions provided in the manual. The quality and integrity of the total RNA were ascertained using a Nanodrop spectrophotometer and an Aglient 2100 Bioanalyzer (Thermo Fisher Scientific, USA). The mRNA was enriched using oligo (dT) magnetic beads and fragmented to synthesize cDNA for PCR amplification. Then, the PCR products were denatured into single strand to develop the single-stranded circular DNA library and sequenced using DNBSEQ (BGI, China).