capped_rep_2
To capture 5' capped RNAs, total RNA was first dephosphorylated to remove 5' phosphorylated species, and then decapped, which resulted in phosphorylated 5' ends of formerly capped molecules only. This approach is based on the TERA-Seq method. The resulting RNAs were processed as 5' phosphorylated RNAs by direct adapter ligation to phosphorylated 5' ends, as in the degradome method. In order to reduce bias caused by sequence-dependent efficiency of ligation 48, the 5' adaptor was a mix of two sequences containing random bases.