GSM9150970: ChIP-seq of HexA in P. carotovorum PCC27 at mid-log phase (replicate 1); Pectobacterium carotovorum; ChIP-Seq
Formaldehyde was added to a final concentration of 1% to cross-link protein-DNA complexes. Cells were harvested, lysed, and chromatin was sonicated to 200–500 bp fragments. HexA-bound DNA was immunoprecipitated using anti-FLAG antibody. Libraries were prepared using the TruSeq ChIP Sample Preparation Kit (Illumina) following the manufacturer's protocol (TruSeq ChIP Sample Preparation Guide 15023092 Rev. B). Paired-end sequencing was performed on the Illumina NovaSeq 6000 platform with a read length of 101 bp.