GSM8778359: Patient-derived organoid 6; Homo sapiens; RNA-Seq
RNA from PDOs were isolated using the AllPrep DNA/RNA Mini Kit (QIAGEN) and concentrations were determined using Nanodrop One C (ThermoFisher Scientific, #ND-ONEC-W). A library of the RNA extracted from PDOs was prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760S/L). Total RNA was depleted from ribosomal RNA (rRNA) using the Qiagen fast select kit (#334387).