GSM8705989: S41_48_M; Anolis sagrei; OTHER
DNA was isolated from the blood cell pellet (majority nucleated red blood cells) using Qiagen's PureGene CELL extraction kit (method described in Lindsey and Schwartz 2022, "DNA Isolation from Reptile Blood using Gentra Puregene (Qiagen) DNA Isolation Kit" published on protocols.io). Libraries were prepared using the NEBNext Enzymatic Methyl-seq kit from New England BioLabs (64) with 200 ng genomic DNA as input. Prior to enzymatic conversion, libraries were sheared to a target fragment size of 300 bp using a Covaris sonication instrument. After assessing library qualities using Bioanalyzer and Qubit instruments, 40 enzymatically converted libraries were sequenced on two lanes of an Illumina Novaseq 6000 instrument at the University of Florida's Interdisciplinary Center for Biotechnology and Research to yield an average of ~120 million paired-end, 150bp reads per sample.