Illumina paired end 2x150 metatranscriptomic raw reads of engorged ticks (Hyalomma marginatum) from wild boar (Sus scrofa). Locality: 300 km from PTiB, Catalonia, Spain
Tick pools were washed three times with 70% ethanol and once with PBS. AVL buffer was then added to each tick pool and ticks were mechanically disrupted using plastic tissue homogenisers. RNA was extracted from tick-AVL supernatant using the QIAamp Viral RNA Mini Kit. Library preparation was done using the Stranded Total RNA-Seq with Ribo-zero Plus Microbiome kit.