Metagenomic sequencing of groundwater microbiome: test sample, 2023-08-28, Sunnyvale CA, replicate 2
16S rRNA gene amplicon libraries targeting the V4 region were prepared using the Earth Microbiome Project primers 515F and 806R and sequenced on an Illumina MiSeq at the ASU KED Genomics Core Facility. Library preparation involved PCR amplification of bacterial and archaeal DNA, followed by paired-end sequencing (2x250 bp). Demultiplexing was performed using CASAVA 1.8.