RNA-seq of MCF-7 cell line under hypoxia
Cells were incubated under hypoxic conditions for 24 h, placed into the RNAlater Solution (Thermo Fisher Scientific, Waltham, MA, USA), and stored at 4C for a maximum of 14 days. Samples were disrupted by a rotor-stator homogenizer (Omni TH, Kennesaw, GA, USA) in a lysis buffer, and total RNA was isolated using the NucleoSpin RNA kit (Macherey Nagel, Dren, Germany). NGS library was prepared using the QuantSeq 3mRNA-Seq Library Prep Kit (FWD) (Lexogen, Vienna, Austria). Sequencing was performed on the NextSeq 500 System (Illumina, San Diego, CA, USA), yielding unpaired 75-bp reads.