RNA-Seq of mus musculus:adult male cerebellum
Using mRNA as a template, a single stranded cDNA was synthesized using random hexamers primers, followed by the addition of buffer, dNTPs, DNA polymerase I, and RNase H to synthesize a double stranded cDNA. The double stranded cDNA was then purified using AMPure XP beads. The purified double stranded cDNA is first subjected to end repair, followed by the addition of an A tail and the connection of sequencing adapters. Then, AMPure XP beads are used for fragment size selection. Finally, PCR amplification was performed and the PCR products were purified using AMPure XP beads to obtain the final sequencing library.