GSM9285181: Untreated and IL-7 treated CD8 T cells Gene expression; Mus musculus; RNA-Seq
8 days or 14 days after the treatment, splenocytes were isolated from the mice and resuspended in ice cold FACS buffer (Ca2+/Mg2+ PBS supplemented with 4% FBS and 2mM EDTA). Splenocytes were stained with antibodies and LCMV-GP33 Tetramer in FACS buffer on ice for 30min. Cells were washed and resuspended in FACS buffer, LCMV-GP33 Tet+ CD8 T cells were sorted using FACSAria II(BD) Library was contructed using manufactures protcol for single cell 5' v2 10x genomics Library prepared occording 10x genomics instructions using 5' library and CITE-seq with TotalSeq-C Cell hash antibodies.