GSM9010932: AC16 cells, CRISPRa, rep 1; Homo sapiens; RNA-Seq
RNA isolation was performed on cells with the RNeasy Plus Mini Kit (Qiagen) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. All library preparation steps were carried out by Novogene