OGD1
PC12 cells were subjected to OGD/R treatment. To establish the OGD/R model, cells were initially seeded in culture dishes and allowed to adhere for 24 hours. The culture medium was then replaced with glucose-free MEM, and the cells were subjected to OGD in an anaerobic chamber (Mitsubishi, Japan) for 4 hours. Following this, the medium was replenished with normal culture medium, and the cells were transferred to a 5 % CO2 incubator for continued culture for an additional 24 hours.