GSM8978339: 2nd_Batch_GEX-8; Mus musculus; RNA-Seq
Tumors were perfused with a solution containing DNase I and Liberase , cut in smaller pieces and incubated for 30 min at 37°C. The suspension was washed with RPMI, 5% FBS and 2 mM ethylenediaminetetraacetic acid , mashed and filtered through a 70 μm cell strainer, twice. Lymph nodes were mashed, filtered and washed in HBSS. Spleen were mashed and red blood cells were lysed by ACK lysing buffer (Ammonium-Chloride-Potassium, home-made). Suspension was neutralized by adding RPMI, 5% FBS and filtered before further manipulation. Librairies were performed using Reagent Kits v3.1 (Dual Index) with surface protein from 10X according to manufactur's instruction