GSM9093893: preterm; Homo sapiens; RNA-Seq
Single cells were suspended in calcium- and magnesium-free PBS containing 0.04% BSA after the peripheral blood mononuclear cells were isolated from the blood samples. Then, single cell suspension went through cell counting and viability assessment using Countess II FL Automated Cell Counter. RNA libraries were prepared with chromium single cell 3 reagent kits v3 according to the manufacturer's protocol.