GSM9415305: Adult_KD4; Mus musculus; RNA-Seq
Frozen hippocampi were thawed on ice and 25-30mg of tissue was used for RNA extraction. Hippocampi were dissociated mechanically using a motor tissue grinder (Fisherbrand®, #12-1413-61) and 1.5mL pestles- RNase-free (KIMBLE, #749521-1590) in lysis buffer (QIAGEN, #1015762) and β-mercaptoethanol. RNA extraction was performed using the RNeasy mini-kit (QIAGEN, #74104) according to manufacturer's instructions. RNA libraries were prepared according to library kit manufacturer's protocol, indexed, pooled, and sequenced on an Illumina NovaSeq 6000.