Metagenomic long-read sequencing of wound debrediment in diabetic foot ulcers.
Samples were extracted with Qiagen Genomic Tip protocol. Long-read sequencing libraries were prepared with the ONT barcoding ligation protocol and sequenced on two MinION flow cells. Fastq data was collected together and screened for any human sequences with direct alignment to the human genome assembly GRCh38.p14 with Minimap2. Reads were cleaned and classified metagenomically using KrakenUniq, and any reads classified as human were further screened.