GSM8704379: 0Gy_DMSO_d5_1-3, 0Gy_Forskolin_d5_1-3, 4Gy_DMSO_d5_1-3, 4Gy_Forskolin_d5_1-3; Homo sapiens; RNA-Seq
At day 5, the gliomaspheres in the suspension culture were collected and dissociated with TrypLE (no phenol red), and the adherent differentiated cells were washed with HBSS and de-attached with Trypsin/EDTA. The cells were then pooled and filtered through a 40-µm strainer and fixed with EvercodeTM Cell Fixation v2 kit (#ECF2101, Parse Biosciences), following the manufacturer's guidelines. Library was performed according to the manufacter's instructions for the EvercodeTM Whole Transcriptome Mini kit (#EC-W01010, Parse Biosciences).