GSM8669096: Oxidized 1; Mus musculus; RNA-Seq
Total RNA was extracted from macrophages and subjected to purification and removal of genomic DNA contamination. Ribosomal RNA was depleted to obtain enriched mRNA for library preparation. Fragmentation and random priming of samples was done for 10 nanograms of mRNA for each sample. First and second strand cDNA synthesis was carried out using NEBNextUltra Dirctional RNA library prep kit for Illumina according to manufacturer's protocol. Equimolar quantities of all libraries were pooled and sequenced in a highthroughput run on the Illumina NovaSeq6000 sequencer using the paired end sequencing format.