GSM9335351: EPD210, Control, 72 hrs, rep 3; Homo sapiens; RNA-Seq
Total RNA was isolated using the RNeasy mini kit (Qiagen) and measured using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific) Libraries for high-throughput RNA-seq analyses were generated using the SMARTer stranded total RNA-seq pico input mammalian kit (Takara Bio) with an input of 10 ng total RNA. The rRNA removal step was followed by a purification step using AMPure XP Beads (Beckman Coulter) and 10 PCR cycles were used for amplification. The final libraries were quality controlled and quantified using the high sensitivity DNA kit on 2100 Bioanalyzer (Agilent Technologies) and the high sensitivity double stranded DNA kit on Qubit 4 Fluorometer (Thermo Fisher Scientific). The libraries were pooled at equimolar concentrations and sequenced on a HiSeq 4000 platform (Illumina) to generate 150 bp paired-end reads