GSM8843125: T-bet-Eomes Double Knockout 2; Mus musculus; RNA-Seq
Total RNA was obtained from day 4 in vitro primed CD4 T cells that were resuspended in TRIzol. RNA was further purified by phase separation using cholorform and RNeasy kit (Qiagen). Cells were Dnase treated to remove impurities. Libraries were prepped using Illumina's Stranded Total RNA Prep Ligation with Ribo-Zero Plus Kit and 10bp unique dual indices (UDI).  Paired end 150 bp reads were sequenced using a NovaSeq X Plus. Bcl-convert (v4.2.4) was used to perform demultiplexing, quality control, and adapter trimming. Sequencing reads were distributed as compressed fastq files.