GSM9366039: Low expression assay time point 6 (72h, dox-treated); Saccharomyces cerevisiae; OTHER
Plasmid DNA was isolated using a protocol combining a freeze-thaw cycle and zymolyase-mediated cell wall digestion, followed by purification with the QIAprep Spin Miniprep Kit (Qiagen). Samples from each timepoint were prepared for next-generation sequencing using a single, 15-cycles PCR. Barcodes were amplified with primers containing overhangs that included sequencing primer, annealing sequences, unique indexes, and P5/P7 Illumina adapter sequences.