RNA-seq of tomato root: early differentiation zone, flg22 treatment, rep 3
Root tissue was collected, snap-frozen in liquid nitrogen and stored at 80C until analysis. Whole root and LD samples (100 mg  10) or ED samples (20 mg  5) of root ground tissue from each sample was used for RNA extraction using Trizol (Invitrogen), following the manufacturers instructions. RNA purification was done with Qiagen RNeasy mini-Kit with DNase I treatment in-column treatment. RNA-Seq analysis was performed using Novogene in-house protocol on the Illumina NovaSeq platform (HWI-ST1276) with paired-end 150bp sequencing strategy. RNA library is formed by polyA capture and reverse transcription of cDNA.