Shotgun metagenomics of the human fecal virome
Starting from human feces sample, viral particles were enriched using ultracentrifugation and treated with nucleases. RNA and DNA were extracted using the PureLink Viral RNA/DNA Mini Kit. RNA was reverse transcribed and second-strand synthesis of cDNA was performed. Both DNA and ds-cDNA were amplified using SISPA. Library was prepared using the DNA Prep (M) Tagmentation Kit and sequenced in an Illumina MiniSeq (PE150 reads).