GSM8809386: ATAC_423_Beyer_Shakiba_2_S2; Mus musculus; ATAC-seq
After sacrificing of the mice, splenocytes and lymph node cells were pooled and CD4+ T cells were enriched by negative selection using the MagniSort Mouse CD4 T cell enrichment Kit according to the manuscript. Subsequently CD4+ T cells were surface stained with anti-CD3, anti-CD4, anti-CD25, anti-CD62L and anti-CD44 and naive CD3+CD4+CD25-CD44low/-CD62L+ T cells were sorted. Naive CD4⁺ T cells were differentiated under TH1 and TH17 conditions. Whole transcriptome, ATAC and sample tag libraries were obtained according to the manufacturer's protocol (BD Biosciences) followed by index PCR to ligate sequencing indices.