GSM9278485: GC_FLX_rep4; Mus musculus; RNA-Seq
RNA was precipitated using TRAP assay, and purificated with Rneasy Micro Kit (QIAGEN). Ovation RNAseq V2 kit (NuGene) was used with 500 pg of RNA for cDNA synthesis. Sequencing libraries were constructed by a TruSeq RNA library Prep Kit v2 (illumina). Libraries were sequenced for 50 bp paired-end on Illumina HiSeq2500 using Rapid V2 sequencing chemistry (Illumina).