GSM8746402: WT-METTL3-CHIP; Mus musculus; ChIP-Seq
Day 6 ROs of Rx:GFP and Mettl3 KO with or without Dox (2 µg/ml) treatment were trypsinized into single cells and then total cell number 2×107 cells for each ChIP were fixed with 10% paraformaldehyde (Sigma) at room temperature for 10 min and quenched the reaction using glycine at final concentration of 0.125 M. The cells pellets were further sonicated to 300–500 bp in SDS lysis buffer by using a Qsonica Biorupter. 15 μg ChIP grade anti-HA antibody (Abcam, ab9110) and anti-METTL3 (Bethyl, A301-567A) were used for ChIP assay. The ChIP-DNA were sent to University of Michigan DNA Sequencing Core for quality control, library preparation, and sequencing using Illumina NovaSeq S4 Libraries platform.