GSM8809853: Mesonephric tissue and gonadal tissue of donkey; Equus asinus; RNA-Seq
The ovary were washed in 1×PBS immediately after dissociation. These samples were stored in MACS Tissue Storage Solution within 48 h (Miltenyi Biotec, Bergisch Gladbach, Germany). It was subsequently transferred to 0.2% collagenase IV and DNase I digestive juices, followed by incubation at 37°C for 15 min. After digestion and mechanical striking into single cells, the cell suspension was filtered through a cell strainer (Solarbio, Beijing, China). DNBelab C series high-throughput single-cell RNA library preparation kit is based on droplet microfluidic technology and independently designed using MGIC4 droplet generation device, through efficient mRNA capture magnetic beads and droplet recognition microbeads and single-cell RNA library preparation kit The single-cell (nuclear) suspension can be quickly prepared into a dedicated library corresponding to the sequencing platform.