GSM8977889: 195-BL; Homo sapiens; RNA-Seq
Total RNA was isolated via poly‑A mRNA enrichment using oligo‑dT magnetic beads as part of the TruSeq Stranded mRNA kit workflow (oligo‑dT capture, wash, and elution) Illumina TruSeq® Stranded mRNA Library Preparation protocol (poly‑A selection, mRNA fragmentation, first‑ and second‑strand cDNA synthesis, end repair and A‑tailing, UMI adapter ligation, two rounds of AMPure XP bead clean‑up, PCR amplification) ​