Leaf_1 (CRX1818273; CRA027237)
There are two main ways to obtain mRNA: one is to enrich the mRNA with polyA tail by Oligo(dT) magnetic beads, taking advantage of the structural characteristics of most eukaryotic mRNA with polyA tail. The second is to remove ribosomal RNA from total RNA to obtain mRNA. Subsequently, the obtained mRNA was randomly interrupted by divalent cations in the NEB Fragmentation Buffer, and the library was built according to the general library building method or chain-specific library building method of NEB.