GSM9270681: Cell surface protein - Lane B; Homo sapiens; OTHER
Tumour and healthy liver tissues were minced and digested in RPMI 1640 with 10% FCS and 0.5 mg/ml collagenase D at 37 °C for thirty minutes, passed through 70 µm strainers, and lymphocytes were enriched using a 44%:70% isotonic Percoll gradient. PBMCs, when collected, were isolated on Ficoll Paque PLUS. Cells were cryopreserved in CryoStor CS10 (liver samples) or 10% DMSO: 90% FCS. Live, CD3+ T cells were sorted via fluorescence activated cell sorting. Libraries were made according to the 10x Genomics protocol (Chromium Single Cell 5' v2)