RNA-Seq Arabidopsis thaliana WT 2-4
Total RNA was isolated using the NucleoSpin RNA Plant Mini kit and treated with DNaseI. RNA quantity and quality were assessed using Qubit4 and agarose gel electrophoresis. cDNA libraries were prepared with the QuantSeq 3 mRNA-Seq Library Prep Kit (Lexogen) and sequenced on the Illumina NovaSeq 6000 platform with 75 bp single-end reads. Four to seven biological replicates were analyzed per plant line.