GSM9278508: Control_Vascular tissues_3weeks; Mus musculus; RNA-Seq
Following arterial digestion, cells were resuspended in MACS sorting buffer containing 0.5% BSA at 1,000 cells/μL. Approximately 10,000 cells per sample were processed using the Chromium™ Single-Cell 3' Reagent Kit (10x Genomics) according to the manufacturer's protocol. Single cells were partitioned into microfluidic chambers on a 10x Genomics Chromium Controller and encapsulated in gel beads containing sequencing adapters, primers, molecular barcodes, unique molecular identifiers (UMIs), and oligo-dT primers. This generated single-cell barcoded droplets (GEMs). After GEM formation, mRNA was reverse transcribed to produce barcoded cDNA libraries.