Genotyping-by-sequencing from Setophaga coronata: blood sample
Genotyping-by-sequencing (GBS) using  the restriction enzyme PstI to fragment genomic DNA. We used BluePippin to size select fragments in the 300-400 nt range. The size-selected GBS libraries were sequenced on a single lane using Illumina NextSeq 2000 (P3 kit) to obtain 150x150 paired reads.