GSM9040965: m6A, AD High 3, Input; Homo sapiens; OTHER
mRNA was first isolated through poly(A) selection using oligo (dT) beads (ThermoFisher) followed by magnetic bead separation from 420 ng of total RNA. The mRNA was DNase treated at 37°C for 10 minutes then immediately sheared into 100-200nt fragments by heating at 95°C for 12 minutes in 1x Turbo DNase buffer (ThermoFisher). Van Nostrand, E.L., et al., Robust, Cost-Effective Profiling of RNA Binding Protein Targets with Single-end Enhanced Crosslinking and Immunoprecipitation (seCLIP). Methods Mol Biol, 2017. 1648: p. 177-200.