Spont_Mut1_sensitive
From a yeast strain with a centromeric defect (cse4-T133A-S135A- E136A cbf1, temperature-sensitive), six spontaneous or UV-induced temperature-resistant derivatives were isolated. Derivatives were backcrossed to a CSE4 CBF1 strain, and temperature-resistant (i.e. with potential causative mutation) and temperature-sensitive segregants (i.e. without the causative mutation) were collected in pools and subjected to WGS.