GSM8843014: 22Rv1_CTC_19; Homo sapiens; RNA-Seq
Cells were stored in a lysis buffer containing poly-ethylene glycol, Triton X-100, RNAse inhibitor, a custom oligo strand, dNTPs, and nuclease free water at -80°C until ready for library preparation according to the Smart-seq3 protocol.  The freeze/thaw aided in cell lysis Libraries were prepared according to the Smart-seq3 protocol (doi: 10.1038/s41587-020-0497-0)