Illumina raw reads of fungal ITS2
Fungal ITS2 region was amplified using primers ITS3-2024F (5- GCATCGATGAAGAACGCAGC-3) and ITS4-2409R (5- TCCTCCGCTTATTGATATGC-3). Sequencing libraries were prepared, indexed, and quantified using Qubit and a real-time qPCR. Library size distribution was assessed using a Bioanalyzer (Agilent, USA). Quantified libraries were pooled and sequenced on the Illumina NovaSeq platform according to library concentration and sequencing depth requirements.