LSEC-H3K27ac-C
Cryopreserved cells were bound to concanavalin A beads and incubated with H3K27ac antibody. Following antibody incubation, chromatin was sheared using pA-Tn5 transposome enzyme and then extracted DNA was used as input for library preparation. H3K27ac histone modification data was generated from cryopreserved cells using the Cut&Tag-IT Assay kit (Active Motif, H3K27ac antibody cat#39133). Library products were assessed by Agilent Bioanalyzer HS DNA assays (Agilent Technologies, Inc.) and clustered on an Illumina Novaseq 6000 S4 flow cell followed by 150bp paired-end sequencing with inclusion of 10-15% spike-in PhiX Control v3 library (Illumina).