Sugiyamaella novakii NCAIM Y.00986 ONT sequencing
For Oxford Nanopore sequencing, genomic high molecular weight DNA was isolated with the Quick-DNA HMW MagBead Kit (Zymo Research, Irvine, CA, USA) according to the company's instructions. Cell lysis was performed with R-Zymolyase (Zymo Research; 50 units of Zymolyase and 5 units of RNase A in 500 l end volume, ~10^8 cells treated at 37C for 60min). Library construction was performed with the Ligation Sequencing Kit (SQK LSK109; Oxford 156 Nanopore, Oxford, UK). The library was sequenced on a MinION R9.4.1 Flow Cell with approximately 2.6 Gb of data before filtering. Super accurate base-calling of the resulting FAST5 sequencing files was performed with Guppy v6.4 software provided by the company.