GSM8844180: DE_LINC01612_KO1; Homo sapiens; RNA-Seq
The cells were washed by DPBS and harvested by buffer RL. The HiPure Total RNA Mini Kit (Magen, Cat# R4111-03) was used to extract total RNA following the manufacturer's instructions and the RNA was quantitated with nanodrop 2000 (ThermoFisher). RNA libraries were prepared using standard protocols, including mRNA enrichment, fragmentation, cDNA synthesis, adapter ligation, and PCR amplification.