small RNA-seq of rattus norvegicus: CK2
Briefly, 3 and 5 adaptors were ligated to 3 and 5 end of small RNA, respectively. Then the first strand cDNA was synthesized after hybridazition with reverse transcription primer. The double-stranded cDNA library was generated through PCR enrichment. After purification and size selection, libraries with insertions between 18~40 bp were ready for sequencing on Illumina sequencing with SE50.