GSM8745400: DOCA_salt-1; Mus musculus; RNA-Seq
Mouse kidney cells treated with CART cells and each control group were isolated. After grinding, cells from each group were dissociated into single-cell suspension. The freshly prepared single-cell suspension was adjusted to 700-1200 cell/μl according to the 10×Genomics Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (No. 1000268) Operation manual for computer and library construction. After reverse transcription, Qubit3.0 was used for preliminary quantification, and the library was diluted to 1ng/ul. Then, Agilent 2100 was used to detect the insert size of the library. After the insert size met the expectation, Q-PCR was performed using StepOnePlus Real-Time PCR System to accurately quantify the effective concentration of the library (the effective concentration of the library was required to be > 10nM) and ensure the quality of the library. Qualified libraries were sequenced using theIllumina Nova 6000 PE150 platform.