Human multiethnic radiogenomics reveals low-abundancy microRNA signature in plasma-derived extracellular vesicles for early diagnosis and molecular subtyping of pancreatic cancer
Total RNA was extracted from plasma samples using exoRNeasy Serum/Plasma Midi kit (Qiagen). Small RNA libraries were prepared using the SMARTer smRNA-Seq Kit for Illumina (Takara Bio), following the manufacturers protocol. The libraries were sequenced on an Illumina NextSeq500 platform, generating single-end reads of 76 bp.