WGS of Klebsiella pneumoniae ST307 and ST5994: clinical isolates from Cantabria (Spain)
Bacterial genomic DNA was extracted from clinical isolates cultured on blood agar plates using Qiagen DNeasy Blood&Tisuue Kit. DNA libraries were prepared with the Oxford Nanopore Rapid Barcoding Kit SQK-RBK114-96 following the manufacturer's protocol, including barcode adapter ligation for multiplexing. Sequencing was performed on an Oxford Nanopore MinION Mk1B using R10.4.1 flow cells. No PCR amplification or size selection was applied.