GSM9362924: Rat normal control striatum, day 3, replicate 3; Rattus norvegicus; RNA-Seq
Ipsilateral striatal tissue was collected from IC, IS, and IS-HA rats, and the corresponding striatal region was collected from normal controls. Total RNA was extracted from approximately 20–30 mg of striatal tissue using the RNeasy Plus Mini Kit (QIAGEN) according to the manufacturer's instructions. mRNA-seq libraries were prepared using the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA). Poly(A)+ RNA was enriched from total RNA using oligo(dT) magnetic beads and fragmented by heating. First-strand cDNA was synthesized with random primers and reverse transcriptase, followed by second-strand cDNA synthesis. Double-stranded cDNA was purified with AMPure XP beads, subjected to end-repair, 3′-adenylation, and adaptor ligation, and then amplified by PCR. Final libraries were quality-controlled using an Agilent BioAnalyzer 2100 and quantified by real-time PCR before sequencing on an Illumina NovaSeq 6000 platform (150 bp paired-end).