GSM8746463: dTAGMTL3-0hr-2; Mus musculus; ATAC-seq
5X104 cells were washed with cold D-PBS and resuspended in 50 μl of cold lysis buffer (CHP-118-C, Boston Bioproducts) and pelleted. Transposition reaction by Tn5 transposase was incubated in TD buffer at 37°C for 30 min. After purification with MinElute Kit (QIAGEN), transposed DNA fragments were amplified through PCR. ATAC-Seq were performed using Nextera DNA sample prep kit (Illumina) by following a protocol described elsewhere.  using barcoded Nextera PCR primers. Extra number of PCR cycles were added by qPCR as described and amplified DNA products were subjected to another round of DNA purification using MinElute Kit. Libraries were sent to University of Michigan DNA Sequencing Core for quality control test based on Bioanalyzer and sequenced using Illuminia Nova-seq 6000 with pair-end reads.