GSM8617987: iSLK_Dox_induced_rep1; Homo sapiens; miRNA-Seq
Total RNA was isolated using mirVana miRNA isolation Kit following the manufacturer's protocol. Approximately 1 μg of total RNA were used to prepare small RNA library according to Illumina's TruSeq small RNA sample preparation protocol (Illumina, San Diego, CA, USA). Then the libraries were sequenced by Illumina's HiSeq 2500 following the vector's recommended protocol. Raw reads were subjected to an in-house program, ACGT101-miR (LC Sciences, Houston, Texas, USA) to remove adapter dimers, junk, low complexity, common RNA families (rRNA, tRNA, snRNA, snoRNA) and repeats.