GSM8843210: Apc-knockout_rep2; Mus musculus; RNA-Seq
Total RNA was extracted with the RNeasy mini kit (Qiagen, 74106) plus on-column DNAse I digestion (Qiagen, 79254) according to the manufacturer's instructions. RNA sequencing libraries were prepared from 500 ng of total RNA using a Quant Seq 3′ mRNA-Seq Library Preparation Kit FWD (Lexogen, Vienna, Austria)