GSM9412747: ChIP_learning_input; Mus musculus; ChIP-Seq
Freshly dissected hippocampal tissue (~20 mg) was crosslinked in 1% formaldehyde for 10 min at room temperature.Chromatin was sonicated for 10 cycles using a Diagenode Bioruptor Pico to generate DNA fragments of 200–500 bp. ChIP-Seq libraries were prepared using KAPA library preparation kit. Indexed libraries were pooled and sequenced in illumina Novaseq6000.