GSM9040990: defenseless_control_1; Arabidopsis thaliana; RNA-Seq
Post-exposure, samples were immediately flash-frozen in liquid nitrogen. The frozen samples were subjected to total RNA extraction using the Plant Spectrum RNA isolation kit. RNA quality was assessed using the bioanalyzer RNA 6000 Nano assay (Agilent Technologies) to determine RNA integrity values. High-quality RNA samples were then employed for complementary DNA synthesis and served as templates for quantitative polymerase chain reaction and transcriptome analysis. RNA-Seq library was prepared using the Illumina Total RNA Library Prep Kit and was quality-checked using a Bioanalyzer (Agilent Technologies) at Novogene, Germany.