16s_rRNA_sequencing_13 (CRX1579531; CRA024201)
he V3-V4 regions were amplified using universal primers 341F and 806R with Phusion High-Fidelity PCR Master Mix (New England Biolabs, USA). Amplification products were purified using AMPure XP magnetic beads (Beckman Coulter, USA). Sequencing libraries were prepared using the TruSeq DNA PCR-Free Library Prep Kit (Illumina, USA), with quality assessment performed through Qubit fluorometric quantification and Bioanalyzer 2112 (Agilent Technologies, USA) size distribution analysis.