Targeted capture of Cygnus buccinator ultra-conserved elements: adult male muscle
We prepared dual-indexed libraries with custom indexes (Glenn et al. 2019) and the Kapa Biosystems Hyper Prep Plus library preparation kit (F. Hoffmann-La Roche AG, Basel, Switzerland) following the manufacturers protocol. We enriched pooled libraries using the myBaits UCE Tetrapods 5Kv1 kit (Daciel Arbor Biosciences, Ann Arbor, MI), optimized for birds and with improved tiling for historical or ancient sources of avian DNA, following the manufacturers protocol. We amplified pools with 16-18 cycles of PCR followed by adapter-dimer removal using a Qiagen GeneRead Size Selection kit.