V4 16S rRNA amplicon sequencing of experimental day 7 broiler excreta after 7 days of aerobic exposure
Fresh fecal sample was collected from the farm and transported on ice to the lab. Metagenomic DNA was isolated with the Qiagen PowerFecal DNA kit (Germantown, MD, USA) following the manufacturer's instructions. A Qiagen TissueLyzer was used for the bead beating step. Extracted DNA was used for the construction of a 16S rRNA gene library following a the wet-lab protocol from the Schloss lab. Briefly, Illumina indexed reads were created using PCR amplification of the V4 region of bacterial 16S rRNA gene. No PCR amplicons were observed in the negative control samples using water as the DNA template.