GSM9249089: 35S:HY5K87K-GFP/hy5 after 2h of the control condition, biological rep3; Arabidopsis thaliana; RNA-Seq
Total RNA was extracted using the Trizol reagent (Invitrogen) and reverse-transcribed into first-strand cDNA using SuperScript II Reverse Transcriptase (Invitrogen). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection