GSM9364137: AB20834; Mus musculus; RNA-Seq
Tumors were finely minced on ice and enzymatically digested in RPMI containing 1 mg/mL Collagenase IV and 0.05 mg/mL DNase I at 37°C for three cycles of 8 minutes each. Between incubations, tissues were mechanically dissociated using a gentleMACS dissociator. 3' end mRNA libraries were prepared for sequencing using MARS-seq (Jaitin et al, Science 2014)