GSM8881774: cKO rep3; Mus musculus; RNA-Seq
Total RNA was extracted from mouse testis using TRIzol reagent (Invitrogen), followed by DNase I treatment. RNA integrity was verified by Bioanalyzer (RIN ≥ 8.0). Briefly, magnetic beads with poly-T oligo were utilized to isolate mRNA from total RNA. After the process of fragmentation, a library for transcriptome sequencing was created and its quality was evaluated using an Agilent Bioanalyzer 2100 system. The index-coded samples were clustered using a cBot Cluster Generation System and a TruSeq PE Cluster kit v3-cBot-HS (Illumina), following the manufacturer's guidelines. After cluster generation, the library preparations were sequenced using the Illumina NovaSeq platform.