GSM9364158: siS1 CM FA-2; Mus musculus; ATAC-seq
Nuclei were extracted from 50,000 cells per sample using lysis buffer (10 mM Tris-HCl [pH 7.4], 10 mM NaCl, 3 mM MgCl2, and 0.01% Triton X-100). Immediately after lysis, the nuclei were incubated in a transposition reaction mix containing Tn5 transposase and tagmentation buffer (Vazyme Biotech) at 37℃ for 30 min. Tagmented nuclei were purified with DNA Clean & Concentrator Kits Kit (Zymo) followed by 9 to 12 cycles of PCR amplification. Amplified libraries were purified with the 1 × AMPure (Beckman) beads, and library size distribution was determined by Bioanalyzer with High-Sensitivity DNA Analysis kit (Agilent Technologies) and sequenced on Illumina NovaSeq (150 bp, paired-end).