GSM9093060: Mut_T3090; Mus musculus; RNA-Seq
Tumors were wrapped in aluminum foil and submerged in liquid nitrogen for 15 seconds, then pulverized using a hammer. A Quick-RNA Miniprep kit from Zymo Research (Cat # R1057) was utilized to extract RNA and sent to Novogene Corporation Inc. for RNA sequencing. All samples were tested for RNA purity using Nanodrop. Potential contamination was evaluated via agarose gel electrophoresis, and sample integrity was further assessed using the Agilent 2100. The cDNA library was constructed from RNA samples, and its quality was assessed prior to Illumina paired-end sequencing. The library preparations were then sequenced using the Illumina platform, generating paired-end reads (2×150 bp, 20M reads/sample).