RNA-Seq of mus musculus: adult male feces
Total DNA was extracted from mice feces using the OMEGA DNA Kit (Omega Bio-Tek, USA). The primers 338F (5'-ACTCCTACGGGAGGCAGCA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') were used to amplify the V3-V4 region of the bacterial 16S rRNA genes. The sequencing was performed using the Illlumina NovaSeq platform according to the protocol of Personal Biotechnology Co., Ltd (Shanghai, China).