GSM9092439: NAIO3-spatial; Mus musculus; OTHER
For spatial transcriptomics sample preparation, eyeballs from NaIO3 mouse and PBS mouse were placed together in the same  embedding mold. Specifically, the eyeball from NaIO3 mouse was positioned in the upper left corner of the embedding mold, while the eyeball from PBS mouse was placed in the lower right corner. It was ensured that the maximum diameters of the two eyeballs were on the same horizontal plane. After sample positioning was completed, the entire embedding space was quickly filled with OCT (Optimal Cutting Temperature compound), and then the sample was immediately placed in liquid nitrogen for rapid freezing. After the rapid freezing process was finished, the samples awaited subsequent sectioning operations. Cryosections of eyeballs were cut at a thickness of 10 μm and mounted onto GEX arrays.Spatial gene expression profiling was performed using the Visium Spatial Gene Expression Slide and Reagent Kit (10x Genomics). RNA libraries of  spatial transcriptomics were prepared for sequencing using standard Illumina protocols.