GSM8810232: P5601_WT_Rep2_FXRChIP; Mus musculus; ChIP-Seq
Liver was dounce homogenized and dual-crosslinked in 2mM DSG for 30 minutes and 1% formaldehyde for 10 minutes and subsequently quenched with glycine. Nuclei were sonicated/lysed in 1% SDS buffer and then subjected to immunoprecipitation with 5ug of FXR (E4B8P, Cell Signaling) overnight. Complexes were isolated with anti-rabbit IgG paramagnetic beads, followed by washing and DNA purification by column (Qiagen Min-Elute). Samples were made into libraries using the KAPA Hyperprep Kit (Kapa Biosystems) and sequenced using the Illumina NextSeq 2000 with 100 bp single-end reads.