Amplicon sequencing of 18S V9 RNA gene of freshwater sediments
We targeted the V9 region of the 18S rRNA gene using 1391F (5'-GTACACACCGCCCGTC-3') and EukBr (5'-TGATCCTTCTGCAGGTTCACCTAC-3'), amplifying a ~96-134 bp region (Stoeck et al. 2010, Choi et al. 2020).