GSM9362384: WT cells with no treatment (technical rep3); Mus musculus; RNA-Seq
Total RNA was isolated from cells using the RNeasy Mini Kit (QIAGEN #74104) following the manufacturer's instructions. RNA quality and quantity were assessed with NanoDrop One and Agilent TapeStation 4200. Libraries were prepared from 200 ng total RNA using the Universal Plus™ mRNA-Seq Library Preparation Kit with NuQuant (TECAN #0520). Sequencing was performed on an Illumina NovaSeq X Plus platform to generate 150 bp paired-end reads (~40 million paired-end reads per sample).