Analysis of the V3/V34 of 16S rRNA gene amplified from DNA extracted from fecal samples of patients with IBD or murine models of IBD sensitized to dietary proteins and feed with enriched diets.
The V3/V34 region of the 16S rRNA was amplified from DNA extracted from fecal content. The primers used for the amplification were modified for adaptation to the Illumina platform and included the addition of 6base pair, unique barcodes to the forward primer, allowing for multiplex amplification. The PCR  products were then sequenced using the Illumina MiSeq platform.