RNA seq of homo sapiens: human CCR6+CD45RO+CD4+ T cells
Strand-specific bulk RNA sequencing libraries were prepared using the Lexogen CORALL mRNA-Seq Library Prep Kit. Total RNA was extracted from CCR6+CD45RO+CD4+T cells and enriched for polyadenylated transcripts using oligo(dT) priming. The libraries were constructed following the manufacturers protocol and sequenced on an Illumina Nova6000 platform using a paired-end layout to assess transcriptome profiles and gene expression.