RNA-Seq turbot: Taurine diet_intestine
RNA was isolated from the head kidney and intestine samples obtained from turbot fed with the control and taurine-enriched diet both in the absence and presence of infection with A. salmonicida (n=4). For this we used the Maxwell RSC simplyRNA Tissue Kit (Promega) with an automated Maxwell RSC 48 Instrument following the guidelines outlined by the manufacturer. RNA concentration was determined using a Qubit 3.0 fluorometer (Invitrogen), purity was assessed with a NanoDrop One spectrophotometer (Thermo Fisher Scientific), and sample integrity was evaluated using an Agilent 4200 TapeStation system (Agilent Technologies). Double-stranded cDNA libraries were constructed using the Fast RNA-seq Lib Prep Kit V2 (ABclonal). Paired-end 150 bp (PE150) sequencing was carried out on an Illumina NovaSeq X Plus sequencer. Both library preparation and sequencing were performed at HaploX (Hong Kong).