GSM8808308: Porphyromonas gingivalis replicate 3; Porphyromonas gingivalis; RNA-Seq
Bacterial cells were fixed with 4% formaldehyde in PBS, permeabilized with lysozyme treatment, and processed for RNA extraction using a modified PETRI-seq protocol. RNA integrity was maintained with RNase inhibitors throughout the procedure. Single-cell transcriptomes were captured using PETRI-Seq, which employs iterative split-pool barcoding to tag transcripts at the single-cell level. The resulting cDNA libraries underwent second-strand synthesis, tagmentation using the Nextera XT DNA Library Preparation Kit, and PCR amplification. Libraries were purified, quantified, and sequenced on an Illumina NextSeq platform.