RNASeq of Mycobacterium tuberculosis Haarlem isolates
culture grown till OD600=0.6, RNA isolation with trizol, RNA purification with AMPure XP magnetic beads (Becton Dickinson), ribosomal RNA was removed with the Ribo-Zero Bacteria rRNA Removal Kit (Illumina), library preparation with KAPA Stranded RNA-Seq kit (Roche)