GSM8705875: YZ-3; Homo sapiens; RNA-Seq
Patients donated a single 18g core biopsy which was either formalin-fixed paraffin-embedded (FFPE) or fresh frozen (FF). RNA was isolated using Qiagen RNeasy FFPE kit (Qiagen, 73504) for FFPE samples, or Qiagen RNeasy kit (Qiagen, 74104) for FF samples. RNA QC (quality and quantity) was performed using an Agilent 2100 Bio-analyzer using Agilent reagents and protocols. RNA-SEQ libraries were generated using Illumina TruSEQ TotalRNA stranded library making kits using Illumina protocols. Library QC (quality and quantity) was performed using an Agilent 2100 Bio-analyzer using Agilent reagents and protocols. RNA-SEQ libraries were sequenced with paired-end 100 bp reads using an Illumina HiSEQ40000 with Illumina reagents and protocols.