GSM9365509: 6h_TPA_Yr2_PIMQ_Rep2; Mus musculus; RNA-Seq
250,000 EpdSCs per condition were FACS-sorted directly into room temperature buffer RLT (QIAGEN) and immediately flash frozen. Two biological replicates were used per condition, with 2-3 mice pooled per biological replicate. Total RNA was isolated with RNeasy Mini Kit (QIAGEN) as per manufacturer's instructions. Libraries were then prepared using Illumina TruSeq standard mRNA library kit and sequenced on Illumina NextSeq using 40-bp paired-end reads. PCR amplified library from genomic DNA