GSM8910727: R292C_Polg_4; Mus musculus; RNA-Seq
Frozen lung tissue was crushed into powder in liquid nitrogen, then total RNA was extracted using a Direct-Zol RNA miniprep kit (Zymo Research, R2072). Stranded libraries were constructed using the KAPA mRNA HyperPrep Kit (Roche Sequencing and Life Science), according to the manufacturer's protocol.  Briefly, the protocol entails isolation of polyA containing mRNA using oligo-dT magnetic beads, RNA fragmentation, first and second strand cDNA synthesis, ligation of Illumina-specific adapters containing a unique barcode sequence for each library, and PCR amplification.  The quality and concentration of the libraries were assessed using the D5000 ScreenTape (Agilent Technologies) and Qubit dsDNA HS Assay (ThermoFisher), respectively, according to the manufacturers' instructions. Libraries were sequenced 150 bp paired-end on an Illumina NovaSeq X Plus using the 10B Reagent Kit.