GSM8617950: HAE1-evolution2-A2; Homo sapiens; OTHER
Cells were harvested to extract genomic DNA after transfection or each round of FACS enrichment. DNA libraries from 293F#209 cells were prepared using an additional linear random barcoded primer extension step with 1-2 μg genomic DNA before the conventional amplicon PCR procedure. This process yielded ~570 bp DNA products, which were gel-purified, quantified by DeNovix, and sequenced by PE300 on NextSeq 2000 platform (GENEWIZ)