Mock-2 (CRX1820121; CRA027304)
Total RNA was extracted from Pseudomonas sp. WCS374 cells that had been co-cultivated with Arabidopsis roots under either mock (control) or PEG-induced osmotic stress conditions. RNA-seq libraries were constructed using the ALFA-SEQ RNA Library Prep Kit II. The protocol involved rRNA depletion, RNA fragmentation, cDNA synthesis, ligation of sequencing adapters, and PCR amplification. The final libraries were sequenced on an Illumina NovaSeq 6000 platform, generating 150 bp paired-end reads.