PacBio HiFi reads from Lingula anatina
PacBio HiFi reads from the brachiopod Lingula anatina.  In brief, 15 g of DNA was extracted from the mantle, lophophore and adductor muscle tissue samples (5 g from each tissue) and was used for DNA library preparations. The genomic DNA was sheared to the desired fragment size using g-TUBEs (Covaris). After removal of single-strand overhangs, damage repair, end-repair and A-tailing, DNA fragments were ligated with the hairpin adaptor. Post-ligation, the library was treated with nuclease, cleaned using the SMRTbell Enzyme Cleanup Kit, and purified with AMPure PB Beads (Beckman Coulter). The desired fragments were subsequently isolated using BluePippin (Sage Science). An Agilent 2100 Bioanalyzer was used to determine the size distribution of the library fragments. The final sequencing of the SMRTbell library was conducted on the PacBio Sequel II platform, utilising Sequencing Primer V2 and the Sequel II Binding 2.0 Kit at Grandomics Biosciences.