Small-RNA seq of scrub typhus patient plasma
5L of total RNA was used as starting material. 3 adapters were ligated to the specific 3OH group of micro RNAs followed by ligation of 5 adapter. Adapter ligated fragment was reverse transcribed with Unique Molecular Index (UMI) assignment by priming with reverse transcription primers. cDNA thus formed was enriched and barcoded by PCR amplification (22 cycles).