Genome sequencing of the evolved phage E12-1 of Xanthomonas phage NP1
The phage genomic DNA was extracted using a commercial DNA extraction kit, followed by quality assessment using a spectrophotometer (e.g., NanoDrop) and agarose gel electrophoresis to ensure high purity and integrity. The sequencing library was prepared using the Illumina-compatible library preparation protocol, including fragmentation of the genomic DNA, end-repair, A-tailing, and ligation of sequencing adapters. The library was then size-selected and amplified via PCR to enrich adapter-ligated fragments. Library quality and concentration were validated using a Bioanalyzer or qPCR. Paired-end sequencing was performed on the Illumina NovaSeq 6000 platform following the manufacturers standard protocols, generating high-quality reads for downstream analysis.