WGBS of sheep: adult female caruncle2
DNA samples were fragmented into 200-400 bp using sonication (Covaris S220, Woburn, MA, United States). Next, end repair, A-ligation, and methylation sequencing adapter ligation was performed. Following this, the DNA library was subjected to bisulfite treatment (EZ DNA Methylation Gold Kit, Zymo Research, Irvine, CA, United States). Library concentration was first quantified by Qubit2.0, diluted to 1 ng/l before checking insert size on Agilent 2100 (Agilent Technologies, Santa Clara, CA, United States), and quantified with more accuracy by quantitative PCR (effective concentration of library > 2 nM). Libraries were then pooled per sample and sequenced paired-end reads from Illumina HiSeq X Ten platform.