ONT of coelomocytes
DNA samples from both animals were prepared for long read sequencing with a SQK-LSK109 ligation sequencing kit (Oxford Nanopore Technologies). Library quantities were examined on a Qubit 2.0 fluorometer after sample preparation using a Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific). Samples were sequenced separately which culminated in eight different Flongle (R9.4.1 or R10.4.1) and four different MinION (R9.4.1 or R10.4.1) flow cells using a MinION sequencing system.