SMRT data of the environnemental isolate AG1 belonging to the Francisellaceae
The strains were cultured in 2 mM FeCl3 MEB at 20C with 190 rpm agitation for four days and 2 mL of broth culture was used to extract DNA. In short, cells were pelleted and suspended in 100 L molecular grade water before DNA extraction using the QIAmp DNA Mini Kit (Qiagen) following manufacturers instructions at exception of the elution performed in the elution buffer (EB) (#... Qiagen). The quality of DNA extracts was checked by Nanodrop 2000 (Thermo Scientific) and the quantification was performed with Qubit 1X dsDNA Assay kit on Qubit Fluorimeter 4 (Invitrogen). Samples were submitted to short-read (Illumina) and long-read (PacBio Sequel II) sequencing. Illumina libraries were prepared using the Nextera CD 24 DNA Library Preparation Kit (Illumina), verified with the Fragment analyzer (Agilent) and sequenced on the iSeq100 sequencer (Illumina) at LABEO laboratory facilities. Depth of sequencing was set at 40X. PacBio Sequel II sequencing was performed by the Gentyane INRAE platform (Clermont-Ferrand, France).