16S rRNA sequencing of mouse fecal samples
The forward primer 341f (a mixture of two sequences: CCTACGGGDGGCWGCAG, CCTAYGGGGYGCWGCAG) and the reverse primer 806r (GACTACNVGGGTMTCTAATCC) were used for amplification. Sequencing was performed on the Illumina NextSeq2000 platform using a 600-cycle flow cell kit to produce 2x300bp paired reads. 30-40% PhiX control (unindexed) was spiked into the library pool to support optimal base calling of low diversity libraries on patterned flow cells