GSM8958610: Spinal cord_Replicate 6; Gnathonemus petersii; RNA-Seq
Total RNA was extracted separately from trigeminal ganglia, brains (cerebella), and spinal cords from 6 individual fish using TRIzol reagent (Invitrogen, cat. no. 15596026), following the manufacturer's instructions. RNA concentration and integrity number (RIN) were assessed by Agilent 2100 Bioanalyzer (Agilent, cat. no. G2946CA). RNA concentrations for all samples were in the range of 5.2–146.1 ng/µl and RIN values were in the range of 7.9–9.5. Libraries were prepared using the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs, cat. no. E6420L).