GSM9271017: WT5; Mus musculus; RNA-Seq
Individual muscle sections were homogenized in a lysis buffer containing 10% BME on a Beadblaster 24R Microtube Homogenizer (Benchmark scientific, NJ, USA.). Total cellular RNA was extracted using the RNeasy® Fibrous Tissue Mini Kit (Qiagen), and RNA quality and quantity were assessed on a NanoDrop® ND-1000 UV-Vis Spectrophotometer. Messenger RNA was purified from total RNA using poly-T oligo-attachedmagnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNAsynthesis After polyA enrichment and quality control, libraries were sequenced on an Illumina NovaSeq6000 PE150 system to obtain paired-end reads of 150 bp.