GSM9277200: Patient 1091; HIF2a ChIP-Seq - normal; Homo sapiens; ChIP-Seq
Using a 2 mm2 core needle, one core was extracted from frozen RP tissue blocks in the areas marked on the corresponding slide. Frozen cores were pulverized using the Covaris CryoPrep system. ATAC-seq: Frozen tissue cores (2 mm2 core, ~20 mg) were pulverized (Covaris CryoPrep), nuclei were isolated via iodixanol density gradient, and 50,000 nuclei were subjected to Tn5 transposition using the Omni-ATAC protocol; libraries were purified, PCR-amplified, and sequenced on an Illumina platform (150 bp paired-end). RNA-seq: Total RNA was extracted from frozen tissue using Qiagen RNeasy columns with on-column DNase digestion, and 1  g RNA was used to prepare stranded polyA+ libraries (Illumina TruSeq); libraries were barcoded, pooled, and sequenced on an Illumina HiSeq 2500 (50 bp paired-end). DNA sequencing libraries were prepared using the ThruPLEX-FD Prep Kit (Rubicon Genomics). Libraries were sequenced on an Illumina HiSeq 4000 to generate 150-bp paired-end reads (Novogene).