Metagenomes of hypoxic sediment microcosm
Preparation of DNA and RNA was done in parallel using the Marchery and Nagel NucleoBondRNA Soil Mini kit in combination with the Macherey-Nagel DNA Set (Dren, Germany) according to manufacturers protocol. Residual genomic DNA in the RNA extractions were removed using the RapidOut DNA Removal Kit as specified by the manufacturer (ThermoFisher Scientific) and the absence of genomic DNA was verified via PCR. Metagenomic libraries were prepared with the Illumina DNA prep protocol according to manufacturers protocol.