GSM9040178: jacP16_cd1cranial_21; Jaculus jaculus; RNA-Seq
Three cranial growth plates per vertebral identity per species was pooled into RNAlater (Invitrogen) and equilibrated overnight at 4˚C. Samples were removed from RNAlater and snap frozen in liquid nitrogen the next day and stored at -80˚C or immediately processed for RNA extraction. Tissue was disrupted over liquid nitrogen using a mortar and pestel, then homogonized using Qiagen QIAshredder system. RNA was extracted using the Qiagen RNAeasy Micro kit with on-column DNA-digest UCSD IGM used Illumina mRNA Stranded Library kits to prepare libraries from samples with Agilent Tapestation 4200 RIN scores >8