GSM8671158: 1425_Lem; Mus musculus; RNA-Seq
Frozen cortical tissue was weighed and homogenized in RNAase-free beaded tubes (REDE, Next Advance) in chloroform with TRIzol™. Samples were centrifuged for 15 minutes at 12'000xg at 4°C and the aqueous upper supernatant transferred for RNA isolation with the RNeasy Mini Kit (Qiagen) following manufacturer's instructions. Takara Clontech SMARTer Ultra Low RNA