16S rRNA V3-V4 region sequencing of human fecal sample
DNA was extracted from stool samples using routine procedures and sent for 16S rRNA sequencing. A V3 - V4 region of the 16S rRNA gene was amplified from 629 samples, including technical duplicates, negative and positive controls, using primers 341F (5'-CCTACGGGNGGCWGCAG-3') and 805R (5'-GACTACHVGGGTATCTAATCC-3').