WGS-Mplutonius
Libraries were prepared using the Roche Kapa HyperPrep DNA Library Preparation Kit with Unique Dual Index adapters following the manufacturers recommendations. Completed libraries were QCd and quantified using a combination of Qubit dsDNA HS and Agilent 4200 TapeStation HS DNA1000 assays. The libraries were pooled in equimolar amounts and the pool quantified using the Invitrogen Collibri Quantification qPCR kit. This pool was loaded onto one (1) lane of a NovaSeq SP flow cell using the Xp loading method. Sequencing was performed in a 2x150bp paired end format using an Illumina NovaSeq 6000 v1.5 300 cycle reagent kit. Base calling was done by Illumina Real Time Analysis (RTA) v3.4.4 and output of RTA was demultiplexed and converted to FastQ format with Illumina Bcl2fastq v2.20.0