SBM-mouse1
Skull bone marrow were dissected from perfused mice, cleaned of meninges, and minced in ice-cold buffer before enzymatic digestion (37C, 20 min) with inhibitor cocktail. After RBC lysis (ACK buffer) and filtration (40 m), viable cells were enriched using Miltenyi Dead Cell Removal Microbeads. Single-cell suspensions in PBS/0.2% BSA were processed for 5' scRNA-seq using the 10x Genomics Chromium platform. Libraries were sequenced on an Illumina NovaSeq X Plus with 5' gene expression profiling.