GSM9092670: Patient_15_Seminoma; Homo sapiens; miRNA-Seq
miRNA from each sample was isolated using the miRNAeasy FFPE kit (Qiagen, Germantown, MD) according to manufacturer protocols. 500 nanograms of total RNA was used as input for the llumina TruSeq Small RNA Sample Kit according to the manufacturer's instructions.  This approach uses a naturally occurring miRNA specific structure with a 5'-phosphate and a 3'-hydroxyl to ligate adapter sequences exclusively to miRNA species. After adapter ligation, reverse transcription, PCR amplification and polyacrylamide gel electrophoresis were performed to generate a library. Library quality and quantity were assessed with the Agilent Bioanalyzer using the High Sensitivity Chip according to manufacturer's instructions.