Single-nucleus RNA seq of murine renal tumors and lung metastases generated by RENCA cells overexpressing Interleukin-34
Single-nucleus RNA seq of murine renal tumors and lung metastases generated by RENCA cells overexpressing Interleukin-34
Fresh samples were quick-frozen in liquid nitrogen and stored at -80°C until the day of nuclei isolation Frozen samples were dounce-homogenized in ice-cold Lysis Buffer containing 0.1% TritonX 17,000 nuclei/sample were processed for Gel Bead-in-Emulsion (GEM) and library generation using Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1 (10X Genomics)