W30-4-3 (CRX1816318; CRA027183)
mRNA with a polyA structure in the total RNA was enriched using Oligo(dT) magnetic beads. The RNA was then fragmented into approximately 300 bp lengths by ionic fragmentation. Using the RNA as a template, the first strand of cDNA was synthesized  with 6-base random primers and reverse transcriptase. Subsequently, the second strand of cDNA was synthesized using the first strand cDNA as a template. submitted_instrument_model: Illumina HiSeq X Ten