GSM8999629: si-control-03_Prostate-Can_VCaP; Homo sapiens; RNA-Seq
To extract RNA from cells - NucleoSpin Reagent Kit from Macherey-Nagel  (Düren, Germany) was used according to the manufacturer's protocol Sequencing libraries were prepared using the Illumina TruSeq mRNA stranded Kit following the manufacturer's instructions. Briefly, mRNA was purified from 500ng of total RNA using oligo(dT) beads. Then poly(A)+ RNA was fragmented to 150 bp and converted to cDNA. The cDNA fragments were then end-repaired, adenylated on the 3′ end, adapter ligated and amplified with 15 cycles of PCR. The final libraries were validated using Qubit (Invitrogen) and Tapestation (Agilent Technologies). 2x 100 bp paired-end sequencing was performed on the Illumina NovaSeq 6000 according to the manufacturer's protocol