GSM9248670: ire1DX, untreated, rep 3; Candida albicans; RNA-Seq
Total RNA was extracted using a MasterPure Yeast RNA Purification kit from LGC Biosearch Technologies. The quality of purified RNA samples was assessed by Bioanalyzer RNA analysis (Agilent; performed by the London Regional Genomics Centre) to ensure an RNA Integrity Number (RIN) of 8 or higher. Final concentration of RNA used for library preparation ranged from 37.3-98.5 ng/µL (1.12-2.96 µg). Library preparation was performed by Azenta Life Sciences. Total RNA was purified using polyA selection and RNA samples were converted to Illumina TruSeq cDNA libraries.