GSM8648027: GB_LT_Patient-16; Homo sapiens; RNA-Seq
RNA isolated with TRIzol (cat. no.: 15596026; protocol no.: MAN0001271 Rev. C.0). Poly-A enrichment using Dynabeads mRNA DIRECT purification kit (cat. no.: 61012; protocol no.: June 2012 Rev. 009) Libraries prepared applying Direct RNA sequencing kit (cat. no.: SQK-RNA002; protocol no.: DRS_9080_v2_revO_14Aug2019) from 600 ng of poly-A enriched RNA. Maunifacturer's protocol was adjusted for multiplexing according to directions made by the Poreplex author (https://github.com/hyeshik/poreplex)