ost1-3-WT2 (CRX1820068; CRA027302)
Total genomic DNA was extracted from root-associated microbiome samples using the PowerSoil DNA Isolation Kit (Qiagen, Germany). The V3-V4 hypervariable region of the bacterial 16S rRNA gene was amplified using the primers 349F (5'-ACTCCTACGGGAGGCAGCA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3'). Sequencing libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, USA). The final libraries were sequenced on an Illumina NovaSeq 6000 platform, generating 2x250 bp paired-end reads.