GSM8746202: 17CT-116_neg.rep1; Homo sapiens; RNA-Seq
PDCOs were lysed and homogenized using TRIzol reagent. Total RNA was extracted from homogenized Trizol-PDCO samples according to the manufacturer's protocol. RNA quality control was performed using the 2100 Expert Bioanalyzer. The QC criteria included a total RNA amount of at least 1.0 μg, a minimum RNA concentration of 20 ng/μl, an RNA integrity number of 7 or higher, an rRNA ratio of 1.5 or higher, and non-degraded RNA. cDNA library was constructed using the TruSeq RNA Access Library Prep Kit.