ONT cDNA-seq of Secale cereale: grain 14 DAP
100 ng of poli(A) mRNA was used for library preparation with the Direct cDNA sequencing Kit SQK-DCS109 (Oxford Nanopore Technologies, Oxford, UK). Sequencing was performed using the MinION device (Oxford Nanopore Technologies, Oxford, UK) and an R9.4.1 flow cell (Oxford Nanopore Technologies, Oxford, UK). Basecalling was performed with MinKNOW UI 24.02.6 in fast mode.