16SrRNA of Montipora capitata's early embryonic development exposed to polyvinyl chloride microplatic leachate
A controlled eco-toxicology assay was conducted to evaluate the effects of polyvinyl chloride (PVC) leachate from microplastics on the early embryonic development of the reef-building coral Montipora capitata. Developing coral embryos were collected and preserverd at 4, 9, and 14 hours post-fertilization in Zymo Research DNA/RNA Shield, then stored at -80C. DNA was subsequently extracted using the Zymo Research Quick-DNA/RNA Miniprep Plus Kit following the tissue prep protocol. Extracted DNA was sequenced by University of Washington's Microbial Interactions & Microbiome Center (mim_c).