Amplicon of 16S: mushroom fruiting body
DNA extracted from mushroom fruiting bodies using modified salt extraction protocol (Aljanabi, S. M. & Martinez, 1997) followed by library preparation using the Illumina protocol. A ~300-350bp fragment was amplified using the 16S primers 515F and 806R (Caporaso et al, 2011). All locus-specific primers were modified to include Illumina adapters. Reverse primers also included a unique 12 bp Golay barcode, primer pad (AGTCAGTCAG) and linker (CC) (Caporaso, J. G. et al, 2012).