GSM8776825: Induced, induced Tet-opsin-VEGFA mice (Input); Mus musculus; ChIP-Seq
The tissue was first crosslinked with methanol before being lysed, and the DNA was fragmented via sonication. A transketolase antibody (Santa Cruz, sc-390179) was used to immunoprecipitate the DNA-protein complexes. The cross-links were reversed, and the DNA was purified and quantified before sequencing. The libraries were prepared following Illumina's protocol. Briefly, 10 ng of DNA was blunt-ended, A-tailed, and ligated to Illumina adapters. The fragments (~200-1500 bp) were enriched via PCR and purified with AMPure XP beads. The library was denatured to single strands, loaded onto a flow cell at 8 pM, and sequenced on an Illumina NovaSeq 6000 (2×150 cycles).