RNA-Seq of Thiobacillus denitrificans (DSM 12475)
First, the total RNA in the extracted sample was used, and the sample was quality controlled using Agilent 2100 bioanalyzer. After the quality control was qualified, the RNA was processed. The cDNA of about 370~420 bp was screened with AMPure XP beads, PCR amplification was performed, and the PCR product was purified again using AMPure XP beads to finally obtain the library. After the library was constructed, the Qubit2.0 Fluorometer was used for preliminary quantification, and the library was diluted to 1.5ng/ul. Then, the insert size of the library was detected using Agilent 2100 bioanalyzer. After the insert size met expectations, qRT-PCR was used to accurately quantify the effective concentration of the library (the effective concentration of the library was higher than 1.5 nM) to ensure the quality of the library.