GSM8775734: Isotype control, 10 mg/kg, replicate 4; Mus musculus; RNA-Seq
RNA was extracted from ~100,000 CD11b+ microglia per sample with the RNeasy Plus Micro Kit (Qiagen, Ref #74034) and resuspended in nuclease-free water for RNAseq library preparation. Libraries were generated using the QuantSeq 3' mRNA-Seq V2 Library Prep Kit FWD (Lexogen 193.384) with the UMI second strand synthesis module, following the protocol defined by the manufacturer. Library quantity and quality were assessed with TapeStation D1000 screentape (Agilent 5067-5582). Libraries were pooled in equimolar ratios for sequencing on one lane of an Illumina NovaSeq X 10B flowcell; 100 bp, single end reads were generated.