GPS5_3
Genomic DNA was extracted from stool samples with the MagAttract Microbial DNA Kit (Qiagen) using a custom automated protocol on the Hamilton Microlab Star. Samples were thawed on ice and a 200l aliquot from the stool sample was used as input for the kit following the manufacturer protocol. Cells were lysed by bead beating on the TissueLyser (Qiagen) at 20Hz for 20 minutes and the final elution volume was 110l. The V3-V4 regions of the 16S rRNA gene were amplified by two-step PCR, with amplicon pooling, sequencing on an Illumina HiSeq 2514 instrument.