GSM8959833: U2OS, non-polyA-TERRA; Homo sapiens; RNA-Seq
Total RNA was extracted from 1 x 10^7 cells using TRIzol™ Reagent (Invitrogen™, Cat:15596018) following the manufacturer's protocol. For Illumina sequencing, the cDNA library was constructed using the NEBNext® Ultra II Directional RNA Library Prep Kit (New England Biolabs, E7760S) according to the manufactory instruction with some modifications. The first-strand synthesis step was carried out by SuperScript IV (Thermo Fisher Scientific, Cat: 18090010),  using 1 μM of telomeric C-rich primer. The rest steps were performed as the protocol provided by manufactory. For Illumina sequencing, the cDNA library was constructed using the NEBNext® Ultra II Directional RNA Library Prep Kit (New England Biolabs, E7760S) according to the manufactory instruction with some modifications. The first-strand synthesis step was carried out by SuperScript IV (Thermo Fisher Scientific, Cat: 18090010).