GSM8745652: primary visual cortex, formal deprivation myopia, 4 weeks; Cavia porcellus; RNA-Seq
Briefly, beads with a unique molecular identifier (UMI) and cell barcodes were loaded close to saturation to form cell-bead pairs in a gel bead-in-emulsion (GEM). After exposure to cell lysis buffer, polyadenylated RNA molecules were hybridized to the beads. The beads were then retrieved in a single tube for reverse transcription. On cDNA synthesis, each cDNA molecule was tagged on the 3' end with UMI and cell label, indicating its cell of origin. The cDNA and cell barcodes were then amplified by PCR. The constructed libraries were quantified using a High-Sensitivity DNA Chip (Agilent Technologies) on a Bioanalyzer 4150 and Qubit High-Sensitivity DNA assay (Thermo Fisher Scientific). scRNA-seq libraries were constructed using the 10x Genomics Single Cell Gene Expression Solution v3.1.