GSM8911822: Female WT MTECs, naïve, biol rep 1; Mus musculus; RNA-Seq
Rneasy mini kit as directed by manufacturer (Qiagen) A. mRNA enrichment, mRNA fragmentation and random priming; B. First and second strand cDNA synthesis; C. End repair, 5' phosphorylation and dA-tailing; D. Adaptor ligation, PCR enrichment and sequencing