="ITS2 seq of Symbiodiniaceae community: "&B408
Coral holobiont DNA was extracted using modified phenol/chloroform/isoamyl alcohol protocol. DNA cleaned with Zymo DNA Clean & Concentrator kit, dilution library prepared prior to targeted PCR amplification of ITS2 region of algal symbiont nuclear rDNA. PCR products cleaned and diluted prior to brief, additional round of PCR to incorporate unique indices.