GSM8777869: CpoxeRNA_EVEN_bio rep2; Mus musculus; OTHER
50 million cells per sample were crosslinked with 3% formaldehyde at room temperature for 30min. Cells were sonicated in the ChIRP lysis buffer with probe sonicator (Branson SFX150 Sonifier) with 30s ON, 45s OFF, 30min total ON time, at 38% J. The following steps were performed according to the protocol from "Chu, C., Quinn, J., and Chang, H.Y. (2012). Chromatin isolation by RNA purification (ChIRP). J Vis Exp. 10.3791/3912.". 1ng DNA was used in library preparation. The sequencing library were prepared with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina.