GSM9040302: Human rheumatoid arthritis knee meniscus; Homo sapiens; RNA-Seq
Human fresh RA synovium tissues were dissected in 2 mm pieces and then digested in collagenase Ⅰ solution (2 mg/mL, Gibco; +10% FBS, Gibco; +1% penicillin-streptomycin, Gibco) for 2 h in 37℃, after which solution was filtered by 70 nm cell strainer (BIOFIL) and centrifuged at 1000 rpm for 5 minutes to obtain cells and made into single cell suspensions. Human fresh RA Meniscal and cartilage tissues were dissected in 2 mm pieces and then digested in collagenase P solution (2 mg/mL for meniscus and 0.25 mg/mL for cartilage, Roche; +10% FBS, Gibco; +1% penicillin-streptomycin, Gibco) for 6–8 h in 37℃ for cell suspensions. Suspension cells were captured with 10x Genomics based on the NovaSeq platform. Fastp was applied with a default parameter for filtering the adaptor sequence and removing low-quality reads.