GSM9151481: S1_ENT; Homo sapiens; OTHER
Fresh frozen tissue blocks were embedded in OCT and equilibrated in the cryostat chamber prior to sectioning. Each block was trimmed and an 8mm x 8mm region of interest was scored and 10um thick section thaw-mounted on to the Visium gene expression slide. Sections that were 10um interior and posterior to the gene expression section were thaw-mounted onto charged slides for H&E and IF staining. Library construction was carried out following the 10x Visium Spatial Gene Expression Reagent Kits User Guide (#CG000239 RevC).