GSM9010246: A375 Control Rep 1; Homo sapiens; RNA-Seq
Approximately 1 million cells were harvested per condition after drug treatment. Cells were centrifuged and lysed using RNA Extracol (EURx) according to the manufacturer's protocol. Lysates were stored at −80 °C and shipped on dry ice to the sequencing facility. RNA samples were processed externally at the Laboratory of Sequencing, Nencki Institute of Experimental Biology, PAS. Libraries were prepared using polyA selection and strand-specific library construction, followed by paired-end sequencing.