CUT&RUN for ER in HCC-1428/LTED cells in HD medium treated with 500 nM entinostat for 1 day, then treated with 1 nM E2 for 1.5 hours
HCC-1428/LTED breast cancer cells in HD medium were treated with DMSO vehicle or 500 nM entinostat for 24 hours, and then treated +/- 1 nM E2 for 90 min prior to CUT&RUN to identify ER binding sites.