GSM8647038: MDA-MB-231 cells treated with compound 28, rep1; Homo sapiens; RNA-Seq
Total RNA was extracted with TRIzol Reagent (Ambion, 15596 026). RNA purification,reverse transcription,library construction and sequencig were performed at ShanghalMajorbio Bio-pharm Biotechnology Co., Ltd. (Shanghai, China) according to the manufacturer's instructions . The RNA-seq transcriptome librariy was prepared following llumina® Stranded mRNA Prep, Ligation (San Diego,CA) using 1μg of total RNA.