GSM8669929: C9-ALS_S13_3; Homo sapiens; RNA-Seq
RNA was extracted from nuurospheres cells using the PureLink RNA Mini Kit (Invitrogen) and converted to complementary DNA (cDNA) using the iScript cDNA Synthesis Kit (Bio-Rad). RNA-seq libraries were prepared with the Kapa Hyper Stranded mRNA library kit (Roche). The libraries were pooled; quantitated by qPCR and sequenced on two SP lanes for 101 cycles from one end of the fragments on a NovaSeq 6000 with V1.5 sequencing kits. Fastq files were generated and demultiplexed with the bcl2fastq v2.20 Conversion Software (Illumina).