GSM9271818: JJN3 ka-kd rep2; Homo sapiens; ATAC-seq
Following centrifugation, cells were lysed and tagmented for 30 min in buffer (33 mM Tris-acetate, 66 mM Potassium acetate, 11 mM Magnesium acetate, 16% Dimethylformamide) containing in-house Tn5 transposase. DNA was then isolated using the Biozol Clean & Concentrator(™)-5 kit. Tagmented DNA was amplified, and Illumina-compatible adapters were added during library preparation. Libraries were purified and sequenced on an Illumina NovaSeq X Plus system with a 25B flow cell in paired-end mode.