GSM8775701: Zea mays B73, root tips (0-1mm), Repli-seq, mid S phase, biorep 3; Zea mays; OTHER
A phenol:cholorform:IAA DNA extraction was used to isolate DNA from each sorted nuclei population. DNA-seq libraries were made using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645S) in combination with NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs, NEB #E6440S). The libraries were constructed using 0.5 ng DNA input; the adapter dilution was 1:25 and there was no size selection performed. The libraries were amplified using 11 PCR cycles. The final average library size ranged from 390-436 bp (240-286 bp sequenceable insert + 150 bp adapters). The libraries were sequenced using the Illumina Novaseq 6000, 150 bp paired end.