GSM9088209: 48TF_Day 9_CD45+_10_BD Rhapsody_exp2_rep2, Brd; Homo sapiens; RNA-Seq
For 10X Genomics: Single cells were isolated using 10x Chromium by 10x Genomics. For BD Rhapsody: sorted cells were loaded per lane onto the BD Rhapsody 8-Lane Cartridge. Subsequent steps were performed using the BD Rhapsody Cartridge Reagent Kit or the BD Rhapsody Enhanced Cartridge Reagent Kit, as specified in the manufacturer's protocol. For 10X Genomics: scRNA-seq libraries were prepared with Dual Index Chromium Next GEM Single Cell 3ʹ Kit v3.1 (Dual Index) (10x Genomics) according to manufacturer's instructions. For BD Rhapsody: Following cell capture bead retrieval, cDNA synthesis was immediately initiated using the BD Rhapsody cDNA Kit, and the BD Rhapsody WTA Amplification Kit was used to prepare the scRNA-seq libraries. Library size and quality check was performed using High Sensitivity D1000 ScreenTape with High Sensitivity D1000 Reagents (Agilent) in a 4200 TapeStation System (Agilent), according to the manufacturer's protocol.