Metagenomic sequencing of human fecal Fe190 (CRX1429636; CRA022242)
Total nucleic acids were extracted strictly using the manufacturer's protocol provided by QIAamp Viral RNA Mini Kit. These nucleic acid samples containing DNA and RNA viral sequences were then subjected to reverse transcription reactions with SuperScript III Reverse Transcriptase with six base random primers, followed by a single round of DNA synthesis using Klenow fragment polymerase.