GSM8808458: Gabaergic neurons nuclei. Control mice 3; Mus musculus; RNA-Seq
Nuclei were isolated from individual cortex frozen in liquid nitrogen and nuclear RNA was extracted as previously described (21) . Nuclear RNA was extracted Nuclei were isolated with a cell sorter. RNA were extracted from sorted nuclei (RNeasy Micro Kit, Qiagen ref 74004) and quantified using Tapestation4150 (Agilent). cDNA preparation: Smaartseq v4 Takara ; cDNA libraries preparation: NexteraXT. Run on Mid output flowcell Nextseq500 Illumina 2x75bp