WGS_BAT93-M822
DNA of the I EMS mutant M3 line BAT93-M822 from the EMS BAT93 population with mutant phenotype for the I gene was extracted from 200 mg of leaf tissue collected from a single plant using QIAGEN DNeasy Plant Pro Kit (QIAGEN, Hilden, Germany). DNA concentration was quantified with NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA) and the DNA quality was evaluated by electrophoresis and visualization on a 1% agarose gel. DNA was sent to Beijing Genomics Institute (BGI, Hongkong Tech Solution NGS Lab) for library construction and whole genome sequencing (WGS) of 150 bp paired-end reads to 24x mean coverage with a median insert size of 342 bp using DNBs (DNA nanoballs) platform.