GSM8978596: P020, Bulk CD4, TCRb; Homo sapiens; OTHER
IFNγ secreting cells were sorted by using IFNγ secretion-detection assay (Mitenyi) before cell sorting on MACSQuant Tyto (Miltenyi Biotec, Germany). IFNγ+CD4+ T cells were lysed in 300µL Lysis/Binding Buffer for Dynabeads™ mRNA Purification Kits and stored at -80°C (A33562, Invitrogen). The TCR products were purified, quantified and loaded on MiSeq or NextSeq1000 instruments (Illumina) for the deep sequencing of theTCRa/b chain.