Shotgun sequencing of human stool
Stool samples were sent to the University of Minnesota Genomics Center for DNA extraction and sequencing. DNA was extracted using Qiagen's DNeasy 96 PowerSoil Pro QIAcube HT Kit following the manufacturer's instructions. PowerBead Pro plates were combined with a TissueLyser II step for sample homogenization and then loaded onto the QIAcube HT for DNA extraction. Samples were sequenced using Illumina platforms targeting 8 million reads per sample (2 x 150 bp).