GSM9040739: Kcnn4 mutant ATAC-seq replicate 2; Mus musculus; ATAC-seq
50,000 cells were pelleted at 500 x g for 5 min at 4°C. Samples were washed in 1 ml cold PBS and resuspended in 50 ul cold cell lysis buffer (10 mM Tris pH7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% NP-40). Nuclei were pelleted at 1000 x g for 10 min and resuspended in 50 ml transposition reaction mixture (25 ml TD buffer, 2.5 ml TDE1, 22.5 ml ddH2O) (20034197, Illumina). Samples were incubated at 42°C for 45 min. DNA was then purified using MiniElute PCR Purification Kit (28004, Qiagen). Libraries were barcoded and amplified with Q5 High-Fidelity 2x Master Mix (M0492S, NEB).