Pacbio Hifi sequencing of Cryptoblepharus pulcher: adult gonad
HMW DNA was sheared to 15-20 kb fragments using a Megaruptor 3 (Diagenode), and size selection was done with a SageELF (Sage Science) system. The resulting DNA extracts were used as input to the SMRTbell Express Template Prep kit 2.0 to prepare libraries suitable for sequencing on the PacBio Sequel II platform. AMPure Beads (Beckman Coulter) were used for clean-ups. Sequencing was done with two single-molecule real-time (SMRT) 8M cells.