Metagenome of aircraft wastewater 4
DNA extracts were diluted 1:10 in RSB buffer (Illumina) and quantified with the Qubit High Sensitivity dsDNA Kit on a Qubit 4 Fluorometer. Libraries were prepared from 200 ng DNA per sample using the Illumina DNA PCR-Free Prep Kit and dual-indexed with IDT for Illumina DNA/RNA UD Indexes. Quantification was performed with the Collibri Library Quantification Kit on a LightCycler 96, and libraries were adjusted to 2.0 nM before pooling. Quality was assessed on a MiSeq 300 cycle cartridge, adjusted to 1.5 nM, and sequenced on an Illumina NovaSeq 6000 (2  150 bp) with 1% PhiX control.