GSM9361717: NMC_Laminin_1; Mus musculus; RNA-Seq
Spheroids were released from the matrix using Cell Recovery Solution (Corning) on ice, washed in PBS, pelleted, and lysed. RNA was extracted using the RNeasy Plus Micro Kit (Qiagen #74034) following the manufacturer's protocol, including gDNA elimination. RNA quantity and integrity were assessed before library preparation. Libraries were prepared starting from 30ng of total RNA and using the TruSeq Stranded mRNA kit (Illumina, San Diego, CA) following the standard protocol (Part#15031047 Rev.E)