DNA-seq of Drosophila melanogaster ISO1 strain: male whole-body
Genomic DNA was extracted from 40 freshly collected Drosophila melanogaster (iso-1) males using the Promega Wizard Genomic DNA Purification Kit (cat # A1120), following the manufacturers protocol. Library preparation was performed at Macrogen (Korea) using the Illumina TruSeq Nano DNA PCR-Free protocol with a 350 bp insert size. Sequencing was conducted on an Illumina NovaSeq X Plus platform, generating 150 bp paired-end reads. The dataset provides high-coverage short-read sequencing for genome assembly and comparative genomic analyses.