Tell-Seq of Trioza urticae: single female individual TUF20
Tell-Seq 51 libraries were constructed using the Universal Sequencing Tell-Seq library preparation kit, using 5 ng input DNA according to the manufacturers instructions with minor modifications: 1 ml of tagging enzyme rather than 2 ml was used, and library cleanup was performed using 0.6-volumes of SPRI beads (Beckman) to optimise library insert size. Two libraries were pooled and sequenced on a NextSeq 550 using High Output Kit v2.5 (300 Cycles: 146 read 1, 18 index 1, 8 index 2, 146 read 2) (Illumina) according to the manufacturers protocol, with custom primers from the Tell-Seq kit.