Hi-C reads from Lingula anatina
Hi-C library from the brachiopod Lingula anatina. In brief adductor muscle tissues were cut into 2 cm segments and cross-linked using a nuclei isolation buffer with 2% formaldehyde. Post cross-linking, tissues were ground to produce a nuclei suspension. The isolated nuclei were digested with 100 units of DpnII and labelled with biotin-14-dATP. Unligated fragments had their biotin removed using T4 DNA polymerase. The DNA was sheared to 300600 bp fragments, underwent blunt-end repair and A-tailing, and was isolated with streptavidin beads. After quality checks with a Qubit Fluorometer and an Agilent 2100 Bioanalyzer, the libraries were sequenced as 150 bp paired-end reads on the Illumina NovaSeq 6000 platform.