GSM8979249: Ela-K; Mus musculus; OTHER
Biopsies were obtained from mouse pancreas, incubated in RNALater (Thermo Fisher Scientific) overnight at 4°C, and frozen in liquid nitrogen. Tissue homogenization was performed by grinding the biopsies in liquid nitrogen with a plastic pestle. Total RNA was extracted using the TRI Reagent (Life Technologies) according to the manufacturer's protocol. RNA was hydrolyzed in bicarbonate buffer and the reaction was stopped by ethanol precipitation. The resulting fragments were then end-repaired and converted into an Illumina library using the NEB Next Small RNA Library Prepkit (Bioke, E7300S), according to the manufacturer's protocol.