GSM8778311: neutrophil,HC,biol rep 1; Homo sapiens; RNA-Seq
Peripheral blood neutrophils were isolated using density gradient centrifugation. Total RNA was extracted using the TRIzol reagent according to the manufacturer's instructions. RNA quality was assessed using the Agilent 2100 Bioanalyzer with RIN ≥7. Total RNA was enriched for mRNA using Oligo(dT) magnetic beads, and mRNA was fragmented to a suitable size (~300 bp). The fragmented mRNA was reverse-transcribed into cDNA using random primers. Libraries were constructed using the Illumina TruSeq RNA Library Prep Kit. Library fragment sizes were approximately 300 bp. Libraries were quality-checked using the Agilent 2100 Bioanalyzer before sequencing on the Illumina NovaSeq 6000 platform (paired-end).