GSM9151159: scRNA_invitrodiff_Naive_TSCM_Day10_Th1_ADT; Homo sapiens; RNA-Seq
On the mentioned day (D10), the cells were taken from culture and washed thoroughly with 1X PBS, and maintained at >95% viability. Cells were stained with hashtag oligos (TotalSeqC, BioLegend) for demarcating donors. scRNA-Seq with 10x Genomics 5' v2. About 25,000 cells per lane were loaded onto Chromium NextGEM Chip K. Manufacturers' recommendations were followed to generate libraries for transcriptome, TCR repertoire and cell surface proteins. Libraries were sequenced on NovaSeq 6000 (Illumina) for 150-bp paired-end sequencing.