GSM9363277: L3, TRAP_rep1; Drosophila melanogaster; RNA-Seq
Lysates were incubated with anti-Flag antibody–conjugated beads to immunoprecipitate Flag-GFP-tagged ribosomes. RNA was extracted using the Direct-zol RNA Microprep Kit (Zymo Research). RNA integrity (RIN ≥ 8) was verified using an Agilent TapeStation. SMART-Seq HT v4 (Takara), Illumina Nextera XT library