NextSeq 1000 paired end sequencing of SAMD00858920
polyA selection, NEBNext Poly(A) mRNA Magnetic Isolation Module (#E7490; NEB, Ipswich, MA, United States); library preparation, NEBNext Ultra II Directional RNA Library Prep (#E7765; NEB); dual index adaptor ligation, NEBNext Multiplex Oligos for Illumina (#E6440; NEB).