WGS of Klebsiella pneumoniae
The DNA was extracted using the TGuide S96 Magnetic Universal DNA Kit (DP812, Tiangen Biotech, Beijing, China), following the manufacturers recommendations.The concentration and integrity of the extracted DNA was assessed using the Qubit 3.0 (Thermo Fisher Scientific, Wilmington, USA), and 1% agarose gel electrophoresis (Tanon, Shanghai, China), respectively.For library preparation, the VAHTS Universal Plus DNA Library Prep Kit for Illumina (ND617-02, Vazyme, Nanjing, China) was used, following the manufacturers recommendations.Library quality control was performed using the QSep-400 (BiOptic, Taipei, China) and Qubit 3.0 (Thermo Fisher Scientific, Wilmington, USA).The qualified library was sequenced on the Illumina Novaseq X platform (Illumina, San Diego, USA) with paired-end 150 bp (PE150) mode. Library construction and sequencing were performed.