Targeted probe captured metagenomics of post-handwashing surface in public washrooms: Laboratory air control
Polywipe was subjected to stomacher and then centrigugation for isolation of microorganisms. DNA extraction was performed by QIAamp BiOstic Bacteremia DNA Kit (QIAGEN, Hilden, Germany), followed by library contruction by NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB, UK). Targeted probe capture was then performed on metagenomic libraries using the Respiratory Pathogen ID/AMR Enrichment Panel Kit (Illumina, San Diego, CA, USA). The captured metagenomic libraries were then sequenced on Illumina Novaseq 6000 (Illumina, San Diego, CA, USA) using paired-end 150-bp reads.