GSM8912024: MT,flesh 2; Ipomoea batatas; RNA-Seq
Total RNA was prepared from flesh and root skin samples using TRIzol reagent kit (Invitrogen, Carlsbad, CA, USA). The concentration and integrity of RNA were measured by an Agilent 2100 bioanalyzer (Agilent Technologies, Palo Alto, CA, USA) and RNase-free agarose gel electrophoresis, respectively. Following the removal of rRNA using the Ribo-ZeroTM Magnetic Kit (Epicentre, Madison, WI, USA), the mRNA was enriched by Oligo (dT) beads. The completed mRNA was then used for library construction following the manufacturer's protocol of NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #7530, New England Biolabs, Ipswich, MA, USA).  All mRNA-seq libraries were sequenced on Illumina NovaSeq 6000 platform in Gene Denovo Biotechnology Company, Guangzhou, China.