RNA-seq of MDA-MB-231 cells: vector
The mRNA was enriched using oligo(dT) magnetic beads, and the mRNA was fragmented by adding interrupting reagent to the enriched mRNA. The interrupted mRNA was used to synthesize one-stranded cDNA and two-stranded cDNA according to the corresponding procedures, and the whole library preparation was completed through the steps of end repair, addition of A-tail, addition of sequencing junctions, purification, PCR amplification, and product cyclization. After passing the library test, DNBSEQ, a BGI high-throughput sequencing platform, was used for sequencing.