GSM8843130: PP Tfh, SPF, rep1; Mus musculus; RNA-Seq
Tfh (CD4+CXCR5+PD1+GITR-CD44+) from the spleen and Peyer's patches of WT mice were sorted by a MoFlo Astrios (Beckman Coulter). Total RNA was extracted with TRIzol (GeneAll) according to the manufacturer's instruction. The library for RNA sequencing was generated using the SMART-Seq mRNA Kit/NEXtera XT DNA Library Preparation Kit (Illumina). RNA-Seq