ATAC-seq of Sus scrofa: FACS-sorted pEPSCs
ATAC-seq libraries from FACS-sorted pEPSCs were prepared using the TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme, TD501). Approximately 50,000 cells were collected, washed with cold PBS, and resuspended in lysis buffer (10 mM Tris-HCl, pH 7.4; 10 mM NaCl; 3 mM MgCl?; and 0.1% IGEPAL CA-630). Nuclei were isolated by centrifugation at 4C, followed by transposition using the kit. The transposition reaction was incubated at 55C for 10 minutes, and DNA was then amplified for 9 cycles. DNA was isolated using the Qiagen MinElute Kit and further purified with the Qiagen PCR Cleanup Kit. Library concentration was quantified using the KAPA Library Quantification Kit (KK4824), and library integrity was assessed by gel electrophoresis.