GSM8881628: skin epidermis, HS1; Homo sapiens; ATAC-seq
HS and healthy skin tissues were cut into small pieces and were digested with 2.5U/ml Dispase-II (Millipores Sigma, Cat#4942078001) in Hanks' Balanced Salt Solution (HBSS, Corning, Cat#21-022-CM) overnight at 4°C. After digestion, the epidermis was peeled off and minced. The minced epidermis was incubated with 0.05% trypsin-EDTA at 37°C for 40 mins for further digestion. The suspension was then filtered through a 40um cell strainer and spun down at 300g at 4°C for 5 mins. Collected single nucleus were for the application of snATAC  sequence. The snATAC-seq libraries were constructed according to manufacture's protocol provided by the Chromium Next GEM single cell ATAC v1.1 reagent kit (10X Genomics).