GSM9149750: Huh7-siCTL; Homo sapiens; RNA-Seq
Huh7 and LM3 cells with and without SRSF11 knockdown were subjected to RNA-seq. Total RNA was extracted from these cells with a TRIzol reagent kit. Next, quality control of the extracted RNA was performed using NanoDrop ND-1000 (NanoDrop, Wilmington, DE, USA) and Bioanalyzer 2100 (Agilent, CA, USA). The captured mRNA was subsequently fragmented using the NEBNextR Magnesium RNA Fragmentation Module (cat. E6150S, USA). The fragmented RNA was used for library construction.