GSM9150128: pc293T, FLAG, Replicate 1; Homo sapiens; OTHER
CUT&Tag was performed using the CUT&Tag-IT® Assay Kit (Active Motif # 53160). The manufacturer protocol was followed with the application of the following antibodies: Histone H3K27me3 (Active Motif #39157), H3K27ac (Active Motif #39134), DYKDDDDK Tag (EpiCypher #13-2031), and Rabbit IgG (Cell Signaling #2729S). Each target was run in triplicate with 2x105 pcHEK293T cells per sample. Libraries were prepped as described in manufacturer protocol for CUT&Tag-IT® Assay Kit (Active Motif # 53160).