GSM9363038: Blood,S16_W0; Homo sapiens; RNA-Seq
Total RNA was extracted and purified from whole blood collected in PAXgene Blood RNA tubes from 30 participants at six time points, including baseline (prior to exposure, Week 0) and every four weeks thereafter until Week 20 post-exposure (i.e., Weeks 0, 4, 8, 12, 16, and 20 using the Total RNA extraction kit (RNeasy kit, Qiagen). The Ribo-Zero Gold kit and TruSeq Stranded Total RNA Sample Preparation Kit (Illumina, Inc., San Diego, CA) were used to deplete rRNA from total RNA and to prepare stranded RNA libraries. Paired-end whole-transcriptome sequencing (100 base pairs) was performed on the NovaSeq 6000 platform (Illumina, Inc., San Diego, CA) at the Clinical Genomics Core Facility in Gothenburg, Sweden.