GSM9091213: EPTB222_batch_5_ROI_9_anno_Other; Homo sapiens; OTHER
Lung FFPE tissue sections were mounted on glass slides, deparaffinized, and rehydrated following the GeoMx DSP manufacturer's protocol. Slides were hybridized overnight at 37 °C with the GeoMx Human Whole Transcriptome Atlas probe set. Tissue morphology markers were revealed by fluorescent immunostaining to guide the selection of ROIs. Barcoded oligonucleotide probes were photocleaved from selected ROIs by UV exposure and collected into separate wells. Oligos were uniquely indexed, and paired-end reads were generated by Illumina sequencing, then aligned and quantified using NanoString's DSP analysis pipeline.