GSM8880829: RNA-BS-seq α-Amanitin treatment; Homo sapiens; Bisulfite-Seq
Total mRNA was extracted by the NEBNext® rRNA Depletion Kit (NEB). 1 μg of the mRNA was used for bisulfite treatment by EZ Methylation Kit (Zymo) following the protocols provided by the manufacturer. After bisulfite conversion, the purified RNA was used for library construction by NEBNext® Ultra™ II Directional RNA Library Prep Kit (NEB).