GSM8842853: uninjured wt; Mus musculus; RNA-Seq
Whole eyes were harvested . The lens, retina, iris, and trabecular network were removed leaving the entire ocular anterior segment, from which most of the conjunctiva was carefully dissected and discarded. Dissect corneas with ice cold PBS and put corneas into one Eppendorf tube filled with 0.7mg/mL liberase TM (from Roche) in RPMI.  Place tube in 37 degree incubator with 5% CO2 for 1 hour. Then add 500ul 0.25%Trypsin for 15min. Use 40um filter into Eppendorf tube. Cell viability was checked and accepted with at least an 80% viability score. Such cells were processed for scRNA-seq using 10XGenomics pipeline in the Northwestern University NUseq Core. follow the instructions from 10XGenomics