GSM9411088: GC Peritoneal metastatic tumor, patient GC20; Homo sapiens; RNA-Seq
RNA was extracted from FFPE blocks or FF tissues using the ReliaPrep FFPE Total RNA Miniprep System (Promega) and RNeasy Mini Kit (Qiagen) for FFPE and FF specimens, respectively, according to the manufacturer's protocol. Nucleic acid yield and purity were assessed using a NanoDrop ND-1000 Spectrophotometer (NanoDrop Technologies, Thermo-Fisher Scientific, MA, USA). RNA libraries were constructed using a TruSeq RNA Access library prep kit (Illumina, San Diego, CA, USA) or Exome capture kit according to the manufacturer's protocols. Briefly, ribosomal RNA (rRNA) was depleted, followed by fragmentation and conversion of RNA molecule to complementary DNA (cDNA) using reverse transcriptase. The single-stranded cDNAs were then converted to double-stranded cDNAs, end-repaired, adenosine-tailed, and adaptor-ligated. The constructed libraries were amplified using polymerase chain reaction (PCR). Subsequently, cDNA libraries were submitted to an Illumina NovaSeq6000 (Illumina, Inc.), and paired-end (2×100 bp) sequencing was performed by Macrogen Incorporated.