Liver-Immune-HHK-B-ATAC2
Cryopreserved cells were pelleted and incubated in Lysis Buffer. Intact nuclei then underwent Tagmentation Reaction and purification. ATAC-seq libraries were generated from cryopreserved cells using the ATAC-seq kit (Active Motif). Library products were assessed by Agilent Bioanalyzer HS DNA assays (Agilent Technologies, Inc.) and clustered on an Illumina Novaseq 6000 S4 flow cell followed by 150bp paired-end sequencing with inclusion of 10-15% spike-in PhiX Control v3 library (Illumina).