sludge of reactor phase 1 (A/O)  in microoxic pre-treatment experiment (CRX1716064; CRA025609)
Total genomic DNA was extracted using the Mag-Bind Soil DNA Kit. DNA extract was fragmented to an average size of about 400 bp using Covaris M220 for paired-end library construction. Adapters containing the full complement of sequencing primer hybridization sites were ligated to the blunt-end of fragments. Paired-end sequencing was performed on Illumina NovaSeq 6003