GSM9039961: ctrl siR + LPS 4hr_1; Mus musculus; RNA-Seq
Samples for RNA-seq were 1 μg in 10 μl, as assessed by the Agilent Bioanalyzer for RNA integrity, prior to library preparation. Digested RNA was purified with standard RNA Phenol/Chloroform/Isoamylic alcohol extraction (RNeasy Mini kit). 300ng of total RNA per sample were utilized as input for mRNA enrichment procedure with 'NEBNext® Poly(A) mRNA Magnetic Isolation Module' (E7490L; New England Biolabs) followed by stranded cDNA library generation using 'NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina' (E7760L; New England Biolabs).