GSM8671078: LNCaP cells, siAR, FOXA1, ChIP; Homo sapiens; ChIP-Seq
Cells or tissues were fixed with 2 mM DSG for 10 min and 1% paraformaldehyde for 10 min followed by quenching with glycine. Chromatin was isolated and sonicated using Diagenode Pico sonicator. ChIP-seq libraries were prepared using NEB Next Ultra II DNA Library Prep Kit for Illumina and amplified libraries purified using NEB purification beads. Libraries were validated using Agilent Tapestation.