GSM8844014: A2929_Rel_pAY15_ c-Rel C&R; Mus musculus; OTHER
The cells were treated with 1.5 μL of pA/G-MNase (#40366, Cell Signaling) and incubated on ice for 1 h. The samples were subsequently washed and resuspended in 50 μL of calcium buffer for targeted digestion. 1 μL of 0.1X Epicypher E. coli spike in DNA (#40366, Cell Signaling) was added. The final mixture subsequently purified according to the manufacturer's protocol (MinElute, Qiagen). Finally, 11 μL of each sample was eluted for analysis for sample quality and sequencing by Novogene. The DNA fragments were repaired, A-tailed and further ligated with Illumina adapter. The final DNA library was obtained by size selection and PCR amplification.