GSM8776483: Del-Xi_c11.8-H3K27ac-CUTandRUN; Mus musculus; Mus spretus; OTHER
CUT&RUN was performed using Patski cells as described in Skene and Henikorff, 2017. In brief, permeabilized Patski cells are incubated with specific antibody, followed by binding of a Protein A-Micrococcal Nuclease (pA/MNase) fusion protein. Upon activation of tethered MNase, the bound complex is excised and released into the supernatant for DNA extraction and sequencing Sequencing libraries were prepared using the Illumina Truseq ChIP sample prep kit. Size selection of the library was done by two-step Ampure bead purification and purification of DNA in the 200-600bp range.