GSM9009056: TIL_Treg_Foxp3_low1; Mus musculus; RNA-Seq
RNA was harvested using TRIzol Reagent (Invitrogen, cat. NO 15596026）. 100-200 ng of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using KC-DigitalTM Stranded mRNA Library Prep Kit for Illumina® (Catalog NO. DR08502, Wuhan Seqhealth Co., Ltd. China) following manufacturer's protocols.