GSM9037350: Pod-TRECK DT 100ng day 5 kidney 1; Mus musculus; RNA-Seq
Total RNA was extracted from whole kidney tissue using the NucleoSpin RNA kit (Macherey-Nagel), following the manufacturer's protocol, including on-column DNase I treatment to eliminate genomic DNA contamination. RNA-seq libraries were constructed using the MGI RNA Library Prep kit, following the manufacturer's protocol for mRNA enrichment via poly(A) selection. Barcodes were ligated for multiplexing. Libraries were assessed for quality and quantity before sequencing.