GSM9150997: WD, L2-crispr, regeneration, rep2; Drosophila melanogaster; RNA-Seq
40 wing discs per sample were dissected in cold Schneider's medium. The Quick-RNA Microprep kit (Zymo Research) was used following manufacturer's instructions, including a 15-minute DNase incubation, to isolate the RNA. The purity and concentration of the resulting RNA were assessed using Nanodrop (Thermo Fisher Scientific) and Bioanalyzer (Agilent Technologies). 500 ng of total RNA were used for reverse transcription. Ribosomal RNA (rRNA) was depleted by using poly-A selection. All libraries were sequenced on an Illumina NextSeq2000 sequencer, using 50-bp paired-end reads. Library preparation and sequencing were performed at the Genomics Unit of the Center for Genomic Regulation (CRG).