Illumina Sequencing of recombinant AAV
Libraries prepared using tagmentation-based and PCR-based Illumina DNA Prep kit and custom IDT 10bp unique dual indices (UDI) with a target insert size of 320 bp and sequenced Illumina NovaSeq 6000 sequencer producing 2x151bp paired-end reads. Demultiplexing, quality control and adapter trimming was performed with bcl-convert1 (v4.1.5).