RNA-seq: microbial depletion in Exaiptasia pallida
Control anemones were maintained in 0.2 micron filter-sterilized artificial seawater for 2 weeks. Total RNA was extracted from each anemone using the RNAqueous Total RNA Isolation Kit (Invitrogen). RNA quantity and integrity were quantified using a NanoDrop ND-1000 Spectrophotometer. Sample aliquots were normalized to a concentration of 10 ng/l submitted to the University of Texas at Austin Genomic Sequencing and Analysis Facility for TagSeq library development and sequencing on a NextSeq 1000.