Hic squence_1 (CRX1546203; CRA023555)
Samples were crosslinked with formaldehyde to stabilize DNA-protein interactions. Following cell lysis and quality control, chromatin was digested with a restriction enzyme, with digestion efficiency verified by electrophoresis. Biotinylated junctions were generated via blunt-end ligation, followed by DNA purification. For library preparation, biotin was enzymatically removed, and DNA was sonicated, end-repaired, dA-tailed, and ligated to sequencing adapters.