GSM8959860: Duct Ligation, L-F127 day 7, replicate 3; Mus musculus; RNA-Seq
At the indictated timepoint, the lacrimal gland was extracted and dissociated with collagenase, hyaluronidase and DNase1 in Advanced DMEM/F12 solution, and live cells were then purified by FACS. Standard 10x Genomics Chromium 3' v3.1 scRNA-seq reagents were used. Approximately 2000-4000 cells were loaded per channel. Cells from one animal replicate were captured per channel. Standard 10x Genomics Chromium 3' v3.1 scRNA-seq RT, cDNA amplification, and sequencing library preparation protocols were followed. Multiplexed sequencing libraries were sequenced on Illumina NovaSeq 6000, averaging about 50,000 reads per cell.