Aligned and Processed TAS of human: blood
Paired-end FASTQ files were aligned to the GRCh38.p14 reference genome with BWA mem (0.7.15) and converted to a BAM file with Samtools view (1.9). BAM files were sorted and indexed with Samtools sort and index. Mate-pair information between mates was verified with Picard FixMateInformation (2.18.29). A read group was added to the sequencing data with Picard AddOrReplaceReadGroups. Lastly, base recalibration is performed with GATK BaseRecalibrator and ApplyBQSR (4.2.6.1)