GSM8879649: SNF2L-1-Input; Toxoplasma gondii; ChIP-Seq
To perform chromatin immunoprecipitation, intracellular parasites of SNF2L were fixed with 1% formaldehyde for 10min. A final concentration of 0.125 M glycine was added to quench the fixation. Then all samples were scraped and syringe-lysed using a 22-gauge needle, filtered by filtration through 3-µm polycarbonate membranes. The purified parasites were lysed using the ChIP lysis buffer (50 mM HEPES, 150 mM NaCl, 1 % NP40, 0.1 % SDS, 0.1 % sodium deoxycholate, 1 mM EDTA, pH 8 and Roche protease inhibitor cocktail) for 30 min and the lysates were sonicated using the bioruptor UCD-200 for 16 min at 4 ℃ with a 30 s on/off cycle. The libraries for sequencing were constructed using the VAHTS Universal DNA Library Prep Kit for Illumina V3 (Vazyme, China).