GSM9363924: TSC_ETiX_DD; Mus musculus; RNA-Seq
ETiXs were washed with PBS and dissociated using TrypLE for 5 minutes at 37°C. After dissociation, cells were counted and resuspended in PBS supplemented with 0.04% BSA to a final concentration of 10,000 cells/μL. Single cell capture and library preparation were performed using the Chromium Next GEM Single Cell 3' Kit with Chip G (10x Genomics), following the manufacturer's instructions.