GSM9041508: GBM005-BT935i-iRFP-T27,Unsorted-repB; Homo sapiens; OTHER
Genomic DNA was extracted using the Wizard Genomic DNA Purification kit (Promega) according to manufacturer's instructions For each sample, the gRNA cassette was amplified directly from genomic DNA using primers harboring Illumina TruSeq adaptors with i5 and i7 barcodes. The resulting sequencing libraries were pooled and gel-purified.