GSM8843372: R2(GC); Mus musculus; RNA-Seq
Total RNA was extracted from the GC-treated group and the control group by the TRIzol method. RNA was quantified, RNA purity was detected by a NanoDrop 2000 spectrophotometer (A260/280 between 1.8 and 2.2), and RNA integrity was detected by an Agilent 2100 bioanalyzer. The mRNAs were randomly disrupted with divalent cations in NEB Fragmentation Buffer, and libraries were constructed utilizing the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (New England Biolabs, Beijing, China).