GSM8669747: H3K4me3-M1-1; Mus musculus; OTHER
Processed samples were incubated with 10 μl of Concanavalin A-coated magnetic beads (NovoNGS® Concanavalin A-coated Magnetic Beads, N251). Bead-bound cells were suspended in Dig Wash Buffer and incubated with antibodies targeting STAT3 (Proteintech, 10253-2-ap), H3K27ac (Abcam, ab4729), or H3K4me3 (Abcam, ab8580) at 4 °C overnight. Subsequently, cells were incubated with secondary antibodies for 60 minutes, followed by incubation with pA-Tn5 adapter complexes for 1 hour. The samples were then resuspended in fragmentation buffer and incubated at 37 °C for 1 hour. DNA was purified using phenol-chloroform-isoamyl alcohol extraction and ethanol precipitation. The sequencing was performed on the Illumina Novaseq6000 platform using 150 bp paired-end reads.