delta_ srbA_2
For library preparation, the mRNA present in the total RNA sample was isolated with magnetic beads of oligos d(T). This method is known as polyA-tailed mRNA enrichment. Subsequently, mRNA was randomly fragmented, and cDNA synthesis proceeds through the use of random hexamers and the reverse transcriptase enzyme. After purification, the resulting products followed end-repair, A-tailing and adapter ligation steps. Fragments of the appropriate size are enriched by PCR, where indexed P5 and P7 primers were introduced, and final products were purified. The library was checked with Qubit 2.0 and real-time PCR for quantification and bioanalyzer Agilent 5400 for size distribution detection. Quantified libraries were pooled and sequenced on the Illumina Novaseq 6000 platform.