GSM8621109: Patient1093, Diagnosis, scRNAseq; Homo sapiens; RNA-Seq
Bone marrow aspirates from one patient at three timepoints (diagnosis, first relapse, relapse/refractory) were collected at the University of Colorado Anschutz Medical Campus after informed consent and protocol approval. Mononuclear cells were isolated using SepMate Ficoll tubes (Stemcell Technologies) and cryopreserved in Iscove M odified Dulbecco medium with 45% fetal bovine serum and 10% dimethyl sulfoxide. Bone marrow samples were thawed, resuspended in DNAse and washed in PBS . A sort using a BD FACSAria (BD) was performed to remove dead cells. Single cells were captured using Chromium X Series (10X G enomics ) and sequen ced on a NovaSeq6000 (Illumina).