GSM9149393: Cohort2-6mice_bolus_immu_PB-VDJ; Mus musculus; RNA-Seq
Single-cell suspensions of FACS-sorted B cells were processed using the 10x Genomics Chromium Single Cell 5' Immune Profiling workflow. Cells were loaded onto the Chromium Controller for partitioning and barcoding, followed by reverse transcription, cDNA amplification, and library construction according to the manufacturer's protocol [10x Genomics Single Cell V(D)J Reagent Kits v2]. The sorted single cells were processed according to 10x Genomics 5' gene expression and B cell Immunoglobulin (Ig) enrichment protocol to prepare the libraries for sequencing.