Illumina shotgun
Shotgun genomic libraries were prepared using the Illumina DNA Prep kit (cat no. 20060060), pooled, and quantitated by qPCR. Sequencing was done on a NovaSeq X Plus with V1.0 kits for 151 cycles (paired-end) on one 10B lane. Fastq files were generated and demultiplexed with bcl-convert v4.1.7. Adaptor sequences (CTGTCTCTTATACACATCT) were trimmed from the 3' end of the reads. Quality scores in the fastq files use the Sanger ASCII offset of 33.