GSM9009016: Case 18 ATRT-MYC primary; Homo sapiens; RNA-Seq
snRNA-seq was performed using the Chromium Single Cell Flex Gene Expression Assay from 10x Genomics. Cells were isolated according to the demonstrated protocol “Isolation of Cells from FFPE Tissue Sections for Chromium Fixed RNA Profiling”, Document Number CG000632 Rev B, from 10x Genomics, using a gentleMACS Octo Dissociator (#130-134-029, Miltenyi Biotec). FFPE scrolls were dissociated using the Enzyme Mix containing Liberase TH (# 5401151001, Millipore Sigma) Probe hybridization and multiplex library preparation were performed using the 10x Genomics Sample Preparation Kit PN-1000414, 16 rxns, according to the protocol “Chromium Fixed RNA Profiling Reagent Kits for Multiplexed Samples”, Document Number CG000527 Rev E, from 10x Genomics. A recovery of 80,000 cells for each library was targeted.