GSM8779363: Uncut Astroides planula 2; Astroides calycularis; RNA-Seq
Total RNA was extracted using the RNAqueous™ Total RNA Isolation Kit (Ambion, Thermo Fisher). Treatment with DNase I (Q1 DNAse, Promega) for 20 min at 37 °C (2 units per sample) was followed by purification using the RNeasy minElute Cleanup kit (Qiagen). RNA quality of all samples was checked using the Agilent 2100 Bioanalyzer with an Agilent RNA 6000 Nano Kit. Library preparation and sequencing were outsourced (BGI, DNBseq), generating 30 million 100-base paired end reads per replicate.