GSM8978631: amygdala, female, batch 1; Mus musculus; RNA-Seq
Nuclei were isolated using the Minute™ single nucleus isolation kit designed for neuronal tissue/cells (Cat# BN-020, Invent Biotechnologies) Chromium Single Cell products: Chromium Next GEM Single Cell 3' Kit v3.1 (Cat#1000269), Chromium Next GEM Chip G Single Cell Kit (Cat# 1000127), Dual Index Kit TT Set A, (GEX Library; Cat# 1000215). The four library constructions were performed according to the manufacturer's instructions. The final libraries were quantified using Qubit 4 fluorometer (Invitrogen).