GSM8669417: cv. Fielder, 1st leaf,  day 0 of Fe deficiency, rep 3; Triticum aestivum; RNA-Seq
Approximately 50 mg of tissue in TRI Reagent® (Merck, Darmstadt, Germany) was homogenised with stainless steel beads and a TissueLyser II (Qiagen, Hilden, Germany). The Direct-zol RNA Miniprep Kit (Zymo Research, Irvine, California, USA) was used to extract RNA according to the manufacturer's instructions. Quality control and RNA-sequencing was performed by the Australian Genome Research Facility (AGRF, Melbourne, Victoria, Australia). Briefly, sample quality was assessed using the Agilent 4200 TapeStation and Agilent RNA ScreenTape (Agilent Technologies, Santa Clara, California, USA) and reagents according to the manufacturer's instructions. Sample concentrations were measured using the QuantiFluor RNA Kit (Promega, Madison, Wisconsin, USA). Library preparation was performed using the Illumina Stranded mRNA library preparation workflow and sequencing was completed with the Illumina NovaSeq X Plus with 150 paired end reads and one lane of a 10B-300 flow cell according to the manufacturer's instructions (Illumina, San Diego, California, USA).