GSM8669253: PAT_Sham_1_wk, rep 6; Mus musculus; RNA-Seq
PAT was excised at the respective study endpoint and lysed with 27.8 µl Quiagen RLT Plus lysis buffer (#1053393) + 1% β-mercaptoethanol per mg PAT via bead homogenisation to yield an RNA concentration of 2-20 ng/µl following the RNA extraction with Zymo Direct-zol RNA MicroPrep (#R2062). Libraries were then constructed with the NEB Next Ultra II FS kit (E6177S, NEB) using the prime-seq specifications