NICD_2nd (CRX1625554; CRA024717)
The wing imaginal discs were dissociated in 0.25 percent Trypsin-EDTA solution at 37 degree Celsius for 10 min. Cells were then washed in DPBS and passed through 35 micron filter before library preparation.Construction of 10x single cell libraries and sequencing on Illumina Hiseq platform were performed by Novogene.