GSM8776417: H1_NPC_MN1-CXXC5_rep2; Homo sapiens; RNA-Seq
For cultured cells, total RNA was isolated using RNAiso Plus reagent (Takara 9108). The integrity of the total RNA was determined with a Bioanalyzer 2100 or 2200 (Agilent). cDNA libraries were constructed using the VAHTS Universal V6 RNA-seq Library Prep Kit (Vazyme). cDNA libraries were sequenced on an Illumina Novoseq using a 150bp paired-end setting.