GSM8775820: STH-2; Ovis aries; RNA-Seq
mRNA and miRNA back-transcription were performed using the HiScript® IiI All-in-one RT SuperMix kit (Vazyme, Nanjing, China) and the miRNA first-strand cDNA synthetic kit (Vazyme, Nanjing, China). We constructed sequencing libraries using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich, MA, USA) following the manufacturer's instructions.