RNA-seq of refractory Aedes aegypti Malpighian tubules: D. immitis infected, 24hrs, rep 2
RNA was extracted using TRIzol and DNAse treated using the Thermo TurboDNase kit. Sequencing libraries were prepared using the NEBNext  UltraTM RNA Library Prep Kit (New England Biolabs, USA) for Illumina per manufacturers instructions with sample-specific indices, and the libraries were sequenced on an Illumina platform using 125bp/150bp paired-end reads.