GSM9011863: 3 month 5xFAD, replicate 1; Mus musculus; RNA-Seq
Mouse forebrain was homogenized in ice cold HBSS to release cells. Homogenate was mixed with Percoll and spun down to get single cell suspension. Cells were stained with anti- CD45 and CD11B fluorescently tagged antibodies, and incubated for 30 minutes. DyeCycle Violet was added and microglia were sorted on the FACS Aria II (live microglia defined as DyeCycle Violet negative, CD45 intermediate, CD11B positive). Cells were then spun down, counted, and submitted for single cell RNA-seq library preparation. Library prepared according to manufacurer's instructions (single cell 3' GEX, 10x Genomics)