GSM8844381: U2OS, H3K27me3, Input, ChIP; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldeyde for 10 mins at room temperature. After quenching and cell lysis, chromatin was sonnicated using Bioruptor Power-up (Diagenode) Sequencing libraries were constructed using the TruSeq ChIP Sample Prep Kit (Illumina, IP-202-1012) as per manufacturer's protocol. Libraries were amplified by 8-12 PCR cycles. qPCR was used to measure the concentrations of each library which were then pooled at equimolar concentration for each lane and sequenced for 101 cycles from one end of the fragments on a IlluminaNovaSeq 6000.