Nanopore_whole_genome_DNA
Genomic DNA (6.25 ng) from a single male was subjected to whole genome amplification using the REPLI-g midi kit (Qiagen, Germany), and the quantity and quality of this amplified DNA assessed in TapeStation system (Agilent 4200) using Genomic DNA ScreenTape (Thermo Fisher Scientific). One aliquot of this DNA (1 mg) was used to construct an Oxford Nanopore SQK-LSK114 library, which was sequenced using a FLO-PRO114M flow cell in the Promethion 2 Solo Nanopore platform (Oxford Nanopore Technologies, Oxford, UK). The data were stored in the POD5 file format for subsequent base-calling and correction using the program Dorado release 0.8.3 (Oxford Nanopore Technologies, Oxford, UK). Sequence reads were stored in the FASTQ format.