RNA-seq of middle size non-coding RNA of Ostreococcus tauri (control condition, replicate 3)
Samples were taken 10.5 and 14.5 hours after dawn. 200ml of exponentially growing culture were centrifuged 20min at 8000g, resuspended in Tri-reagent buffer from RNA Direct-Zol (Zymo research) kit and subjected to mechanic lysis (2*[45s, 30Hz]). Subsequent extractions were done according to manufacturer recommendations. Size selection (50-300 nt), removal of 5m7G cap by Cap-Clip Pyrophosphatase treatment, libraries preparation and subsequent paired-end Illumnia Next-Seq sequencing (2 * 75 bp) were performed commercially (Fasteris, Switzerland).