RibOxi-Seq of homo sapiens neuron
Total RNA was subjected to mRNA isolation using the NEBNext Poly(A) mRNA Magnetic Isolation Module, followed by fragmentation and oxidation. Oxidized RNA fragments with 2-O-methylated terminal nucleotides were selectively enriched prior to library construction. RNA 3'-adaptor sequence is 5-/5rApp/NNcacaCTGTAGGCACCATCAATGACAG/3ddC/-3; RNA 5'-adaptor sequence is 5-/5Biosg/rArCrArCrUrCrUrUrUrCrCrCrUrArCrArCrGrArCrGrCrUrCrUrUrCrCrGrArUrCrUrNrNrNrNrNrNrNrNrNrN-3. Samples 1 and 2 were sequenced in single-end mode, whereas Samples 3 and 4 were sequenced in paired-end mode.