GSM9040305: sgAnkrd11, D5; Mus musculus; ATAC-seq
50,000 cells per library were incubated with Lysis Buffer to lyse cellular membranes. A tagmentation mix containing Tn5 transposome was added and incubated at 37°C for 30 min, followed by Stop Buffer treatment to terminate the reaction. Fragmented DNA was purified using ATAC DNA Extract Beads. DNA libraries for ATAC-seq were prepared using Hyperactive ATAC-Seq Library Prep Kit following manufacturer's protocols