GSM9092434: CD36 flox-2; Mus musculus; RNA-Seq
For smart-seq2, retinae from wildtype and CD36 cko mice were dissected and dissociated into single cell suspension. Cells were then stained at room temperature for 30 minutes with CD45-APC (559864, BD Pharmingen™) and CD11b-BV421 (101236, BioLegend) antibodies. CD11bintCD45⁺ cells were sorted using a FACSAria Fusion cell sorter (BD Biosciences) for smart-seq2. Library quality was assessed with an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA) and sequencing was performed on NovaSeq X Plus platform.