GSM8746305: RNA_dTAG_GPATCH1-dTAG_E14_Rep1; Mus musculus; RNA-Seq
Total RNA was extracted using TRIzol reagent (Invitrogen) according to the manufacturer's protocol Human DLD-1 total RNA was included as a spike-in control and mixed with the extracted RNA samples for normalization during downstream sequencing analysis. Ribosomal RNA (rRNA) was removed. The remaining RNA was used for subsequent sequencing libraries preparation with Stranded mRNA-seq Lib Prep Module for Illumina (Abclonal).