Metagenomes of thermokarst system
Soil and fresh water from thermokarst systems were collected in summer. Freshwater was filtered onto PES 0.22m strerivex filter MiliporeSigma, Germany). Filters were preserved in DNA/RNA shield (Zymo Research Corp., California, USA). Soil and filters were preserved at -80C pending extraction. DNA was extracted with the Zymobiomics DNA/RNA microprep extraction kit (Zymo Research Corp., California, USA), following the manufacturers instructions. Library preparation, PCR and tagmentation were done at Integrated Microbiome Resource using Illumina Nextera Flew kit. Hundred and fifty (150) pb pair-end sequencing was run for 1 X coverage on a NextSeq2000 Illumina platform (Illumina, Inc. California, USA)