GSM8956472: A549 cells, Bud, 1h, rep4; Homo sapiens; RNA-Seq
Total RNA was extracted using the NucleoSpin RNA Extraction kit (MN-740955, Macherey-Nagel) and cDNA prepared from 0.5μg of RNA. RNA sequencing libraries, from 4 independent experiments, were prepared using Illumina TruSeq Stranded mRNA Library Prep kits with the poly(A) mRNA magnetic isolation module as described by the manufacturer. Libraries were validated by D1000 Screen Tape assay on an Agilent 2200 TapeStation system and quantified using Kapa qPCR Library Quantification kits.