Gsin Thorax3 (CRX1829110; CRA027546)
mRNA was enriched using oligo(dT)-conjugated magnetic beads. Fragmentation Buffer was added to randomly fragment the mRNA. First-strand cDNA synthesis was performed using mRNA as the template, followed by second-strand synthesis, with subsequent cDNA purification. The purified double-stranded cDNA underwent end repair, A-tailing, and adapter ligation, followed by size selection using AMPure XP beads. Finally, PCR amplification was performed to enrich the cDNA library.