GSM8619035: sorted KIR+CD8, pool 2; Homo sapiens; RNA-Seq
Sorted cells were captured with beads in  microwell plate using the BD Rhapsody Cartridge Reagent Kit (no. 633731). Library preparation were completed using the BD Rhapsody cDNA Kit (no. 633773) and BD Rhapsody™ TCR/BCR Multiomic Assay for Targeted kit (no. 666622). Briefly, cells captured by beads went through cell lysis, bead retrieval, cDNA synthesis, template switching and Klenow extension, and library preparation following the BD Rhapsody protocol. Libraries were prepared for sample tags, targeted mRNA, and TCR-seq.