GSM8808010: siSHMT2-2; Homo sapiens; RNA-Seq
we took 1.5 μg of qualified total RNA for RNA-seq library preparation, VAHTS mRNA capture beads 2.0 (N403, Vazyme, Nanjing, China) was used to capture and purify total RNA VAHTS Universal V8 RNA seq Library Prep Kit for Illumina (NR605, Vazyme) was used to prepare RNA-seq chain specific libraries through fragmentation, first strand synthesis, second strand synthesis, end repair, linker ligation, amplification, purification, and other steps. Finally, we used Qubit 4.0 (ThermoFisher, Waltham, Massachusetts, USA) for library concentration quantification and stored the libraries at -80 ℃ in a refrigerator.