GSM8807581: HT1080-siQ61K-1; Homo sapiens; RNA-Seq
Total RNA was extracted from HT1080 cells and purified with EZ-press RNA Purification Kit (Cat#B0004D, EZBioscience) according to the manufacturer's instructions. Whole transcriptome RNA sequencing (RNA-seq) was carried out by Novogene with a total amount of 1 µg RNA per sample. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and sequenced on an Illumina Novaseq 6000 platform.