The genomic read data of Bischofia polycarpa
For the Illumina short-reads sequencing process, qualifed gDNA was randomly fragmented using the Covaris LE220-plus Focused-ultrasonicator (Covaris, Woburn, MA, USA). Subsequently, the paired-end Illumina sequencing library with an insert size of 350 bp was generated using TruSeq Nano DNA HT sample preparation kit (Illumina) using the following procedures: (a) library construction begins with genomic DNA that is subsequently fragmented; (b) Blunt-end fragments are created; (c) fragments are narrowly size selected with sample purification beads; (d) A-base is added; (e) dual-index adapters are ligated to the fragments  and final product is ready for cluster generation; (f) ligated product is amplified and ready for cluster generation. Next, the qualified library sequenced on an Illumina Novaseq 6000 platform (Biomarker Technologies Co., Ltd., Beijing, China) with 150 bp paired-end reads. Raw data were filtered by the fastp v0.20.0 (https://github.com/OpenGene/fastp) 14 using the following strategies: (a) filtered reads with adapters; (b) trimmed reads off low-quality bases at the 5-and 3-ends; (c) filtered reads with unknown bases more than 10%; (d) filtered duplicated reads due to polymerase chain reaction amplification; and (e) filtered reads with low-quality bases (Q  5) more than 50%. Finally, yielding 110.15 Gb of high-fidelity clean data. These data were subsequently employed for genome size estimation and heterozygosity analysis.