Target capture and sequencing of Canis lupus: Sample ID AK15048T
We prepared libraries according to Meyer & Kircher (2010) with the following modifications: we used a Covaris E220 to randomly fragment DNA to a mean size of 300 bp (125 l sample volume in a microTUBE Snap-Cap AFA Fiber, 140 W peak incident power, 10% duty factor, 200 cycles per burst, 55 second treatment time). For every post-reaction solid phase reversible immobilization clean-up step, we used Serapure beads (Faircloth & Glenn, 2011). We used unique indexing oligos on both the P7 and P5 ends for each sample to avoid any cross-sample contamination from tag-switching during post-pool PCR amplification steps.