GSM8669386: MONO, CTCF (CUT&RUN), rep 2; Homo sapiens; OTHER
CUT&RUN was performed according to the published protocol by Skene and Henikoff with some modifications. Experiments were performed with primary monocytes and macrophages using antibodies for CTCF (Cell Signaling), SMC1 (Bethyl), PU.1 (Santa Cruz), and EGR1 (Bethyl). Approximately 250-500, 000 cells were used. For digestion, 1.5uL of pAG-mnase (Cell Signaling) was used. The alternative DNA extraction method was used to extract the final DNA product. Sequencing libraries were generated with the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB).