GSM9248943: Twist KO tentacle; Nematostella vectensis; RNA-Seq
Single-cell RNA-seq libraries were prepared from dissociated Nematostella vectensis tentacle and neoplasm tissues using the 10x Genomics Chromium Single Cell 3′ v2 platform. Libraries were sequenced as paired-end reads (2 × 150 bp) on an Illumina NovaSeq 6000 instrument and processed with Cell Ranger v5.1.0. Libraries for sequencing were prepared using the 10x Genomics Chromium Single Cell 3′ v2 pipeline for scRNA-seq according to the manufacturer's instructions.