GSM8669107: Infected-3; Chlorocebus sabaeus; RNA-Seq
Total RNA was extracted from the cells. mRNA enrichment was performed using NEB mRNA enrichment kit Fragmentation and random priming of samples was done for 15 nanograms of mRNA for each sample. First and second strand cDNA synthesis was carried out using NEBNextUltra Dirctional RNA library prep kit for Illumina according to manufacturer's protocol. Equimolar quantities of all libraries were pooled and sequenced in a highthroughput run on the Illumina Novaseq sequencer using 1X50 bp single-end reads and a 1% PhiX spike-in control.