GSM9040310: 11-OPMRI; Mus musculus; RNA-Seq
The right middle lobe of the lung tissues was immediately placed in RNAlater solution, then held on ice for 4–5 h, and then stored at -80°C until the RNA extraction procedure. RNA extraction was performed using RNeasy Mini Kits from Qiagen (Valencia, CA). RNA concentrations were measured with a NanoDrop One (ThermoFisher). One microgram of total RNA was reverse transcribed to cDNA using Bio-Rad iScript cDNA synthesis kit (Hercules, CA) according to the manufacturer's instructions. RNA Quality control, library preparations, sequencing reactions, and initial bioinformatic analysis were conducted at GENEWIZ, LLC (South Plainfield, NJ, USA). RNA samples were quantified using Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA) and RNA integrity was checked using Agilent TapeStation 4200 (Agilent Technologies, Palo Alto, CA, USA). RNA-sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer's instructions (NEB, Ipswich, MA, USA).