GSM9092298: HEK293T-EF1α-mCherry, tRNALeuCAG overexpressed(tL), 1, ncRNA; Homo sapiens; ncRNA-Seq
Total RNA was subjected to small-RNA (< 200nt) extraction using the MirVana Isolation Kit (Thermo Fisher Scientific). The enriched small RNAs were then deacylated in 100 mM Tris-HCl (pH 9.0) and 1 mM EDTA at 37 °C for 30 min. tRNA libraries were constructed with the GenSeq® Small RNA Library Prep Kit (GenSeq, Inc) according to the manufacturer's instructions.