Bacterial 16S community from 1xPBS solution and reagents used from the PowerBiofilm extraction kit.
Total DNA from 1xPBS+kit reagents was extracted using Qiagen PowerBiofilm kit. The V4 region of the 16S DNA gene were amplified using primers 515F/806R and sequenced on a MiSeqsystem with v2 chemistry to obtain paired-end 250-bp reads.