GSM8807610: HCT8 cells, Control,H3K9me3(ChIP); Homo sapiens; ChIP-Seq
Cells were fixed with 37% formaldehyde for 10 minutes. After quenching and cell lysis, chrmatin was sonicated using a SimpleChIP® Plus Sonication Chromatin IP Kit(CST,56383S) Libraries were constructed using the Rapid Plus DNA Lib Prep Kit for Illumina V2 (RK20255, ABclonal, China). Procedures included DNA end repair, 3'-end adenylation, adapter ligation, and PCR amplification.