GSM8881224: Ponce-51T; Homo sapiens; OTHER
Genomic DNA samples were obtained from the FFPE prostate tumor tissues as described in the manufacturer's instructions (QIAamp DNA FFPE Tissue kit, Qiagen, Germantown, MD). We used 7-50 ng of genomic DNA as starting material. DNA was enriched by labeling and capturing as described previously in Song et al., 2011 (https://www.nature.com/articles/nbt.1732). The 5hmC DNA was amplified using universal primer (New England Biolabs, USA), index primer (New England Biolabs, USA) and HiFi HotStart ReadyMix (KAPA/Roche). Single-end 75 bp sequencing was performed on an Illumina NextSeq 500. 22 prostate tumors and 24 adjacent normal FFPE samples were sequenced after 5hmC enrichment.