Shotgun metagenomics of the human fecal virome
Starting from human feces sample, viral particles were enriched using ultracentrifugation and treated with nucleases. RNA and DNA were co-extracted using the QIAamp Viral RNA Mini Kit. RNA was reverse transcribed, and both DNA and cDNA were amplified using SISPA. Library was prepared using the DNA Prep (M) Tagmentation Kit and sequenced in an Illumina MiniSeq (PE150 reads).