cytb metabarcoding of stream bottom sediment: eDNA field sample
eDNA extracted using the Qiagen Powersoil kit from stream bottom sediment was amplified with cytb metabarcoding primers (Snyder and Stepien et al. 2020) targeting fsh species with Nextera stye overhangs, and dual indexed with Nextera indices following the Illumina 16S metagenomics protocol (Illumina Part# 15044223 Rev B). Samples were 250 bp paired-end sequenced on an Illumina MiSeq v3.