GSM8617914: T47D, shZHX2, PA, hypoxia,replicate 2; Homo sapiens; RNA-Seq
RNA was extracted from T47D cells utilizing the FastPure Cell/Tissue Total RNA Isolation Kit. The quality of the total RNA was assessed using an Agilent 2100 Bioanalyzer. This was followed by two rounds of polyA+ RNA selection, after which the mRNA was converted into cDNA. Illumina sequencing adapters and barcodes were added to the cDNA through PCR amplification. The indexed libraries were subsequently normalized and pooled. Finally, 150 bp paired-end sequencing was conducted on the Illumina Nova-seq platform, following the recommended protocol.