RNA-Seq of endometrium tissues
Single cell samples were loaded onto the 10 Genomics platform, where microfluidic technology was utilized to encapsulate cells together with Cell Barcode-labeled beads within droplets. Droplets containing cells were collected, followed by cell lysis within the droplets, allowing mRNA within the cells to become associated with the Cell Barcode on the beads, forming Single Cell GEMs. Reverse transcription reactions were conducted within the droplets to construct cDNA libraries, with sample index sequences on the library enabling differentiation of the source of target sequences. Sequencing was performed on the Illumina NovaSeq X Plus platform with an average depth of 40,000 reads per cell.