WGS of Yamuna: Local rice cultivar O. sativa ssp. indica cv. Yamuna of WB, India
Library preparation was performed using QIASeq FX DNA Library Preparation protocol. About 40 ng of Qubit quantified DNA was enzymatically fragmented, end-repaired and A-tailed in a one-tube reaction using the FX Enzyme Mix provided in the QIASeq FX DNA kit. The end-repaired and adenylated fragments were subjected to adapter ligation, whereby index-incorporated Illumina adapter was ligated, to generate sequencing library. The library was subjected to 6 cycles of Indexing-PCR (Initial Denaturation at 98 C for 2 min, cycling (98 C for 20sec, 60 C for 30sec, 72 C for 30sec) and final extension at 72 C for 1min) to enrich the adapter-tagged fragments.Finally, the amplified library was purified using Cambrian Bioworks CamSelect NGS (#CBWD010) followed by library quality control check. Illumina-compatible sequencing libraries  were quantified by Qubit fluorometer and its fragment size distribution was analyzed on Agilent 4150 TapeStation.