GSM8957925: Kidney, Uninfected Hamster, Day3, replicate 4; Mesocricetus auratus; RNA-Seq
Liver and kidneys tissues of hamsters were resuspended in QIAzol lysis reagent (Qiagen) with a stainless-steel bead of 5 mm (Qiagen) and homogenized with a Qiagen TissueLyser (two cycles of one-minute at 25 Hz, with a one-minute break between cycles in ice). Total RNA was extracted from the homogenized samples using the RNeasy Mini Kit (Qiagen), with a DNAse digestion (Qiagen) incorporated into the protocol. In the case of the in vitro condition of L. interrogans, the harvested exponentially growing bacteria culture were resuspended in QIAzol lysis reagent, and total RNA was extracted using the RNeasy Mini Kit with a DNAse treatment. RNA libraries for RNA-seq were prepared using Illumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus prep kit with the addition of custom primer that target L. interrogans rRNA following manufacturer's protocol.