MiSeq air
Amplicons were generated using high-fidelity AccuPrime DNA polymerase (Invitrogen, Carlsbad, CA, USA), purified using the AMPure magnetic bead capture kit (Agencourt, Beckman Coulter, MA, USA), and quantified with a QuantIT PicoGreen fluorometric kit (Invitrogen). Purified amplicons were then pooled in equimolar concentrations using a SequalPrep plate normalization kit (Invitrogen), and the final library concentration was determined via a SYBR Green quantitative PCR (qPCR) assay.