S50.C2
Genomic DNA was extracted from rhizosphere soil samples using the DP712 DNA Kit (TianGen, China) and from root nodule samples using the CTAB method. The V3-V4 region of the bacterial 16S rRNA gene in rhizosphere samples was amplified using primers 338F (5'-CCTAYGGGRBGCASCAG-3') and 806R (5'-GGACTACNNGGGTATCTAAT-3'), while the V5-V7 region of the endophytic bacterial 16S rRNA gene in root nodules was amplified using primers 799F (5'-AACMGGATTAGATACCCKG-3') and 1193R (5'-ACGTCATCCCCACCTTCC-3').