BCG delta-zmp1 delta-nuoG
The genomic DNA sample was fragmented into short fragments. These DNAfragments were then end-polished, A-tailed, and ligated with full-length adapters for Illumina sequencing before further size selection. PCR amplification was then conducted. Purification was then conducted through the AMPure XP system (Beverly). The resulting library was assessed on the AgilentFragment Analyzer System (Agilent) and quantified to 1.5 nM through Qubit (ThermoFisher Scientific) and qPCR. The qualified libraries were pooled and sequenced on Illumina platforms.