Ileal microbiota of 6-week-old broiler chickens
Bacterial DNA was isolated from the intestinal contents of the ileum and cecum of 6-week-old broiler chickens using the GeneMATRIX Stool DNA Purification Kit. Sequencing libraries were prepared using the 16S Barcoding Kit (SQK-16S114.24; Oxford Nanopore Technologies). Sequencing was performed on an R10.4.1 flow cell (Oxford Nanopore Technologies, Oxford, UK) installed in a MinION Mk1B device.