UNCOVERseq-pool off-target nomination, biological replicate 1
UNCOVERseq (adapted, IDT) genome-wide off-target nomination; pooled nucleofection of 20 Alt-R sgRNAs (original 20x configuration: TET2, RC3H1 + 18 shared) with dsODN tag into HEK293-Cas9 (ATCC CRL-1573-Cas9); gDNA extracted 96 h post-nucleofection; xGen DNA Library Prep EZ UNI + Deceleration Module; rhPCR two-step amplification off integrated dsODN tag.