3 month GWI Organoids: 1046-GWI-Vehicle, 1046-GWI-Toxicant, 1039-Control-Vehicle, 1039-Control-Toxicant, Mixed FLEX scRNA-seq
2 pools of 4 samples each were made. Libraries were then tested for quality utilizing an Agilent Bioanalyzer High Sensitivity chip (Agilent, 5067-4626) to determine average size of the library. Library quantification was performed with the NEBNext Library Quant Kit for Illumina kit (New England Biolabs, E7630L).