RNA-seq of Mus musculus: fetal liver-derived alveolar macrophage M1
Total RNA was extracted using RNeasy Mini Kits (Qiagen), according to the manufacturers instructions with genomic DNA removed from samples using a Message Clean kit (GenHunter, Nashville, TN). Clean RNA was resuspended in 10 L diethyl pyrocarbonate (DEPC)-treated water and shipped to Novogene (Sacramento, CA). Here, RNA quality was appraised using an Agilent 2100 Bioanalyzer and samples with an acceptable RNA integrity number were used for cDNA library production and RNA sequencing using the HiSeq 2500 system to produce 150 bp transcriptome paired-end reads.