RNAseq of AW71 evolved isolate L12: M9 + PCA
Strain was cultured overnight in LB medium, washed and resuspended. Baffled flasks containing 25 mL M9 minimal medium + 30 mM PCA were inoculated to an initial OD600nm of 0.1. Flasks were incubated at 225 rpm and 30C. When cultures reached mid-exponential phase (OD600nm ~1.0-1.5, between 12 and 24 h), 2 mL of cells were pelleted at 6000 xg for 1 min, flash frozen on liquid nitrogen, and frozen at -80C.