RNA-Seq of mus musculus: adult male liver
Total RNA was used as input material for the RNA sample preparations. Briefly, mRNA was purified from total RNA by using poly- T oligo- attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in First Strand Synthesis Reaction Buffer( 5 X) . First strand cDNA was synthesized using random hexamer primer and M- MuLV Reverse Transcriptase, then use RNaseH to degrade the RNA. Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and dNTP.