Human TCR beta
generic amplicon design system for NGS technologies, called GAM-E, that allows designing amplicon-based genotyping approaches for any DNA target.The system is based on three PCRs: i. PCR-1 captures the target region of interest, ii. PCR-2 adds the elements needed for sequencing (P5, NSP1, UMI and barcode in the left end and P7 in the right end), and iii. PCR-3 does the final generic unbiased amplification based on P5 and P7. Only the primers of PCR-1 are target-specific. The rest of primers are generic and common for any target. REFERENCE: Tobes, Raquel and Pareja, Eduardo and Pareja-Tobes, Eduardo, GAM-E: Generic Amplicon Design System for NGS. Its Application to the Design of Human Beta, Gamma and Delta TCR Repertoire Profiling Systems. Available at SSRN: https://ssrn.com/abstract=4604819 or http://dx.doi.org/10.2139/ssrn.4604819