RNA-seq of the water-treated (mock) mutant plants (Osfid1) at 0 hours rep3
Total RNA was extracted with TRIzol (Invitrogen). Quality was checked on a Bioanalyzer 2100 and agarose gel. mRNA was enriched using Oligo(dT) beads, then fragmented and reverse?transcribed to cDNA via NEBNext Ultra Library Prep Kit (NEB #7530). cDNA fragments were end?repaired, A?tailed, adapter?ligated, purified with AMPure XP beads (1.0), and PCR?amplified.