Transcriptome
Total RNA was extracted by a RNAprep Pure Plant Plus Kit (TIANGEN, Beijing, China, DP441) with three biological replicates of root samples according to the manufacturers protocol. RNA quality and integrity were assessed in an NanoDrop (Thermo Fisher Scientific, USA) and Agilent 2100 Bioanalyzer (Agilent Technologies, USA), respectively. All RNA samples were processed with high quality, as documented by RNA integrity number value > 7.0. The complementary DNA libraries for RNA-seq were constructed with a TruSeq RNA Sample Prep Kit (Illumina Inc.). Cluster preparation and PE150 read sequencing were performed according to Novaseq 6000 guidelines (Illumina Inc.).