RNA-Seq of Auxenochlorella pyrenoidosa: control biological replicate 1
Total RNA of samples was extracted using Trizol reagent. RNA concentration and purity were detected by Nanodrop ND-2000 (Thermo Scientific, USA) and Agilent 4150 (Agilent Technologies, CA, USA). After quality control, the mRNA was purified using Oligo(dT)-coated magnetic beads and fragmented. The double-stranded cDNA was adapter-ligated for amplification of PCR. Afterwards, PCR products were purified (AMPure XP system) and the library quality was assessed using Agilent Bioanalyzer 4150 system.