16S rRNA gene sequencing of Mus musculus: adult male gut metagenome-Rep2
Sequencing libraries were generated and indexes were added. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries were pooled and sequenced on NovaSeq6000 or DNBSEQ-G99 platforms, according to effective library concentration and data amount required