Full-length 16S rRNA amplicon sequencing of Trifolium hybridum rhizosphere
Rhizosphere samples were collected from clover plants grown in the UIUC greenhouse. Plants  were inoculated with soil from either the long-term N-fertilized plots or control plots collected from the Kellogg Biological Station Long-term Ecological Research Site (KBS LTER). This was done to assess bacterial community composition between plant compartments under different nitrogen treatments. DNA was extracted from each sample, and full-length 16S rRNA (27F to 1492R) amplicons were generated using the PacBio Kinnex 16S kit. Libraries were sequenced using PacBio Revio to obtain high-fidelity (HiFi) reads for community profiling.