AAC Synergy RNAseq Treatment 134_1
At 5 dpi, the topmost two spikelets (one floret / 6 kernels) were harvested for RNA extraction and sequencing. The floret was cut from the plant using scissors sterilized in 70% ethanol, and flash frozen in liquid nitrogen. The samples were ground into a fine powder in liquid nitrogen in a ceramic mortar and pestle to isolate RNA using the Qiagen Rneasy Mini Kit (USA) following the manufacturers instructions. delivered to Novogene Corporation (USA) for library construction and sequencing with a depth of 60 million paired-end 150 base pair reads by using the Illumina NovaSeq system