Genome sequencing of Bacillus siamensis strain DR_14-266
Genomic DNA for nanopore sequencing was prepared using PureLinkTM Genomic DNA Mini Kit (Invitrogen, Calsbad, CA, USA) from cells grown in 3 ml of LB medium at 37C overnight. Nanopore sequencing libraries were prepared using SQK-RBK004 kit (Oxford Nanopore Technologies, Oxford, UK). Genome sequencing was carried out using FLO-MIN106 (R9.4.1) flow cells on MinION Mk1C under MinKNOW v21.11.7 software with fast basecalling and barcoding enabled according to the manufacturers instructions. Demultiplexed FAST5 files were basecalled using Guppy  v6.1.3 with dna_r9.4.1_450bps_hac model.