RNA-Seq of Hesperophylax consimilis: thoracic tissue
RNA was extracted from paired silk glands and thoracic muscle of Hesperophylax consimilis using a Trizol extraction method and extract quality was assessed with a bioanalyzer. Libraries were constructed via poly-A enrichment and reverse transcription into double-stranded cDNA. PE150 sequencing was performed by Novogene Corporation (Sacramento, CA, USA) on an Illumina NovaSeq X Plus Series. The submitted files consist of sequencing reads in FASTQ format before adapter trimming or read filtering.