Full length nodA amplicon sequencing of Trifolium repens symbiotic nodule
Nodule samples were collected from clover plants grown in the UIUC greenhouse. Plants were inoculated with soil from either the long-term N-fertilized plots or control plots collected from the Kellogg Biological Station Long-term Ecological Research Site (KBS LTER). This was done to assess bacterial community composition between plant compartments under different nitrogen treatments. DNA was extracted from each sample, and full-length nodA amplicons were generated using the Fluidigm kit. Libraries were sequenced using PacBio Revio to obtain high-fidelity (HiFi) reads for community profiling.