16S rRNA gene amplicon sequencing of methane-oxidizing enrichment culture from field-grown rice
Genomic DNA was extracted using the Qiagen DNeasy Blood & Tissue kit (catalog no. 69506), following the manufacturers protocol for Gram-positive bacteria. The extracted DNA was PCR amplified using dual-index 16S V4-V5 primers 515F/926R. A peptide nucleic acid (PNA) PCR clamp was used to block the amplification of plastid and mitochondrial DNA.