16S of microbial biofilm from maple (acer saccharum) leaves
DNA from microbial biofilms on submerged maple (acer saccharum) leaves was extracted using DNeasy PowerSoil Pro Kits (Qiagen). The V4 region of the bacterial 16S rRNA gene was amplified using the EMP 16S Illumina amplicon protocol with primers 515F (Parada et al 2016) and 806R (Apprill et al 2015), and pooled amplicons were sequenced on an Illumina NextSeq instrument using paired-end sequencing.