Pooled distal intestinal contents of GIFT Nile tilapia fed a diet with C:N ratio 7.3, cage 1
DNA was extracted from pooled distal intestinal contents of four GIFT Nile tilapia from one cage. The bacterial 16S rRNA V3-V4 region was amplified using primers ACTCCTACGGGAGGCAGCA and GGACTACHVGGGTWTCTAAT with a two-round PCR protocol. Paired-end libraries were sequenced on an Illumina NovaSeq 6000 platform.