smallRNA-seq of Bactrocera tryoni: female ovaries
Ovary tissue was dissected from 10 virgin females. Total RNA was extracted using TriReagent (Sigma) and submitted to the Western Sydney University NGS Facility. Small RNA libraries were constructed using the TruSeq  Small RNA Library Preparation kit (Illumina) and run on the NovaSeq 6000 with 1  75 bp single reads.