RNA seq of Xenia sp. coral surface mucus t0X23
DNA was extracted from coral surface mucus swabs and water filters using the Qiagen DNeasy Blood & Tissue Kit, following the manufacturers protocol. The V3V4 region of the 16S rRNA gene was amplified using primers 341F (5CCTACGGGAGGCAGCAG3) and 806R (5GGACTACHVGGGTWTCTAAT3). PCR products were normalized using the SequalPrep Normalization Plate Kit (Thermo Fisher Scientific), pooled equimolarly, and sequenced on the Illumina MiSeq platform using 2  300 bp paired-end chemistry (MiSeq v3 kit).