Long-read sequences for rhizobium leguminosarum
We followed standard protocols using Zymo Quick-DNA HMW MagBead Kit (Irvine, California) to extract high molecular weight DNA from 1000 uL of sample. DNA was sent for PacBio HiFi long-read sequencing at the W. M. Keck center at the University of Illinois, where gDNAs were sheared with a Megaruptor 3 to an average fragment length of 10kb then converted to barcoded libraries with the SMRTBell Express Template Prep kit 3.0