RNA-Seq of Helicobacter pylori 26695: pH 5.5 for 4h
Approximately 500 ng of RNA sample was used for ribodepletion by using NEBNext rRNA depletion Kit(Cat. no. NEB E7850X), which includes Probe hybridization of RNA, RNase H digestion, DNase 1digestion according to the manufacturers protocol The NEBNextUltra II Directional RNA Libraryprep kit for Illumina (Cat. no. E7760L), was used to construct double-stranded cDNA libraries, usingRNA fragmentation, first strand cDNA synthesis with random primers, second strand cDNA synthesis,end repair of double stranded cDNA, adapter ligation, removal of excess adapter using samplepurification beads, PCR enrichment of adapter ligated DNA and clean-up of PCR products.