Full-length 16S rRNA amplicon sequencing of soil from KBS T7R2N core 3
Soil samples were collected from the long-term fertilized and control field plots at the Kellogg Biological Station Long-term Ecological Research Site (KBS LTER) to assess bacterial community composition under different nitrogen treatments. DNA was extracted from each sample, and full-length 16S rRNA (27F to 1492R) amplicons were generated using the PacBio Kinnex 16S kit. Libraries were sequenced using PacBio Revio to obtain high-fidelity (HiFi) reads for community profiling.