L5 metagenomic reads
Soil samples covered with sugarcane bagasse leftovers and the control bulk soil were frozen in liquid nitrogen and pulverized with an oscillating ball mill (TE-350, Tecnal Inc.). The homogenized samples were used for microbial DNA extraction using the FastDNA 50 mL SPIN Kit for soil (CAT:6560600. MPBIO) according to manufacture instructions. DNA purification was performed using PowerClean DNA Clean-Up Kit (Mo Bio Laboratories) and gel electrophoresis using 0.8% (w/v) agarose gel was used to separate the DNA fragments and to evaluate DNA quality. The DNA solution was used for metagenomics library preparation using the Nextera Library Preparation kit (Illumina Inc.)