16s rDNA metagenome: of estuarine water
The microbial 16S rRNA V3-V4 region was amplified by 341F (5CCTACGGGNGGCWGCAG 3) and 805R (5GACTACHVGGGTATCTAATCC 3) primers targeting the hypervariable ~460 bp region [22].Paired-end read libraries (2x300 nt) were sequenced on Illuminas (Illumina, San Diego, California, United States) MiSeq platform using the Herculase II Fusion DNA Polymerase Nextera XT Index V2 Kit.