PacBio sequencing of Sulfitobacter pontiacus MB1 genome
PacBio long-read sequencing: multiplexed microbial libraries were prepared, using SMRTBell Express Prep Kit 2.0 (Pacific Biosciences, Menlo Park, CA). A single PacBio sequencing library was constructed that was used for SMRTbell template preparation as described in the protocol (Pacific Biosciences, Menlo Park, CA). The resulting SMRTbell template was annealed to the sequencing polymerase, using a Sequel binding kit 2.0. Sequencing was conducted with a 12pM on-plate concentration using a Sequel I System in CLR mode and a single SMRT Cell (1M; PacBio). PacBio raw reads (polymerase reads) were processed using the SMRT Link software (v8.0).