DNA-Seq of Crinum latifolium: chloroplast genome
Leaf tissue was ground into a fine powder in liquid nitrogen, and approximately 30 mg of the tissue powder was used for genomic DNA extraction. Total genomic DNA was isolated using a modified CTAB method and subsequently purified with the Monarch Spin gDNA Extraction Kit (T3017, New England Biolabs, MA, USA). DNA quality was assessed via 1% agarose gel electrophoresis and the NanoDrop spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). DNA concentration was quantified using a Qubit 4 Fluorometer (Thermo Fisher Scientific, USA). Next-generation sequencing was conducted on the PacBio Onso platform (KTest Co., Ltd., Ho Chi Minh City, Vietnam) to generate 150 bp paired-end reads.