Full-length 16S rRNA amplicon sequencing of Trifolium repens bulk soil
Bulk soil samples were collected from clover plants grown in the UIUC greenhouse. Plants  were inoculated with soil from either the long-term N-fertilized plots or control plots collected from the Kellogg Biological Station Long-term Ecological Research Site (KBS LTER). This was done to assess bacterial community composition between plant compartments under different nitrogen treatments. DNA was extracted from each sample, and full-length 16S rRNA (27F to 1492R) amplicons were generated using the PacBio Kinnex 16S kit. Libraries were sequenced using PacBio Revio to obtain high-fidelity (HiFi) reads for community profiling.