RNAseq of HEL: DMSO
Total RNA was isolated using TRIzol reagent from 110 6 cells in DMSO group, and 1 g was used for library construction via the KAPA mRNA HyperPrep Kit. The process involved magnetic oligo-dT enrichment, fragmentation, and strand-specific cDNA synthesis with random hexamer primers. Following adapter ligation and size selection of 300400 bp fragments, the libraries were validated and sequenced on the Illumina NovaSeq X platform to generate 150 bp paired-end reads.