B cell
Total RNA was extracted from mouse B cells and plasma cells under different genetic or treatment conditions. RNA quality and integrity were assessed using a NanoDrop 8000 spectrophotometer and an Agilent 2100 Bioanalyzer. Libraries were prepared using the Illumina TruSeq Stranded mRNA Library Prep Kit, following the manufacturer's protocol. Polyadenylated RNA was enriched using oligo-dT magnetic beads and subsequently fragmented. First-strand cDNA synthesis was performed using random hexamer primers, followed by second-strand synthesis incorporating dUTP to preserve strand specificity. After end-repair, A-tailing, and adapter ligation, libraries were PCR-amplified and quality-checked. Sequencing was performed on the Illumina NovaSeq 6000 platform with 2  100 bp paired-end reads.