RNA-seq of Bactrocera tryoni: female head and thorax
Head and thorax tissue was dissected from 10 virgin females. Total RNA was extracted using TriReagent (Sigma) and submitted to the Western Sydney University NGS Facility. Host rRNA was depleted in a portion of the sample using Ribo-Zero Plus (Illumina) and the stranded RNA library made with TruSeq Stranded Total RNA Library Preparation kit (Illumina).  The library was run on the NovaSeq 6000 (Illumina) with 2  101 bp paired ends.