RNA-Seq
Our librarys preparation and sequencing were entrusted to BIOMARKER in Beijing, China. Process unfolded as follows: Initially, RNA sample quality underwent testing. Afterwards, the library was constructed and assessed for quality through Qubit 2.0, Agilent 2100, and Q-PCR. Once the library passed inspection, various libraries were amalgamated in line with target offline data, then sequenced with the Illumina NovaSeq platform. Clean Data was obtained by filtering off-board data, and Mapped Data resulted from sequence comparison with the reference genome. Eventually, comprehensive analysis onto mRNA, miRNA, and lncRNA was conducted.