miRNA-Seq of mus musculus: adult male liver
Following sample quality control, libraries were constructed using the NEB Next Multiplex Small RNA Library Prep Set for Illumina (NEB E7300L). Leveraging the unique structural features of small RNAs (a 5-phosphate group and 3-hydroxyl group), total RNA was used as input material for direct adapter ligation to both ends of small RNAs. The ligated products were then reverse-transcribed into cDNA.Subsequent steps included:PCR amplification of cDNA, size selection of target DNA fragments via PAGE electrophoresis, gel excision and purification to obtain the final cDNA library