RNA-Seq of testis: Control
Total RNA was isolated using the TRIzol reagent, followed by purification with PureLink RNA Mini Kit columns according to the manufacturers protocol. RNA concentration and purity were assessed using a Qubit Fluorometer, and integrity was confirmed on an Agilent TapeStation, with all samples showing an RNA Integrity Number (RIN) greater than 9.5. High-quality RNA samples from control and treatment groups (n = 2 per group) were used for library preparation with the KAPA RNA HyperPrep Kit. RNA sequencing (RNA seq), a high-throughput technique for transcriptome profiling, was performed on the Illumina NovaSeq 6000 platform, enabling accurate quantification of gene expression, identification of novel transcripts, and analysis of alternative splicing events.