RNA-seq of wheat grains at 6 DAP
The PacBio high-throughput full-length transcriptome is based on the PacBio sequencing platform. It uses extracted total RNA as starting material, constructs a dumbbellshaped (SMRTbell) library with fragment sizes ranging from 0 to 10 kb, and after passing quality control, proceeds to PacBio sequencing followed by downstream data analysis and result interpretation.