Rainbow Trout Fishmeal Replacement
Input total RNA was visualized on a TapeStation 4200 (Agilent) and all but three samples showed excellent RNA quality with RIN-scores > 8 (three samples with RIN scores of 6.8, 6.9 and 7.7 respectively). Samples were quantified on a Qubit 2.0 Fluorometer (ThermoFischer) and 500 ng of total RNA was used as input for the TruSeq stranded mRNA library kit (Illumina) following the manufacturers manual. Resulting libraries showed a fragment size distribution of around 300 bp and were 2x75 bp paired-end. Sequencing was conducted on the HiSeq 4000 (Illumina) with 10 samples / lane.