HCC-targeted NGS of Homo sapiens: AK448-W8 plasma
Plasma DNA was extracted using JBS plasma cfDNA extraction kit (Cat# 08874, JBS Science Inc., Doylestown, PA). DNA from 1-2 mL plasma was subjected to library DNA preparation using the xGen cfDNA & FFPE DNA library prep kit (Cat# 10010207, Integrated DNA Technologies, Coralville, Iowa), which contains unique molecular identifies (UMIs), and then to an HCC-targeted next generation sequencing (NGS) assay following the manufacturers instructions (JBS Science Inc.).