Swanson PacBio 16S canine fecal microbiota
Amplicon sequencing of 16S rRNA gene using the PacBio Kinnex platform. DNA was amplified using primers AGRGTTYGATYMTGGCTCAG and RGYTACCTTGTTACGACTT targeting bacterial 16S rRNA gene regions. Resulting sequences were processed using the TADA Nextflow workflow with DADA2 (v1.22) for denoising and chimera removal, and taxonomy was assigned using the RDP classifier with SILVA 138.1 database. MSA and phylogeny were done using DECIPHER and FastTree.