TaSPL13
genomic DNA was extracted from young wheat leaves. TaSPL13-HaloTag fusion protein was expressed with the TNT SP6 High Yield Wheat Germ Protein Expression System (Promega, USA). and purified using HaloTag Beads (Promega, USA).The gDNA DAP-seq library was prepared by ligation of short DNA sequencing adapters to purified and fragmented gDNA. Recombinant proteins were then incubated with the wheat genomic DNA library in incubation buffer for 2 h at 4C with slow rotations. The beads were washed five times with 200 L of PBS+NP-40 (0.005%) and re-suspended in PBS buffer. The supernatant was discarded, 25 L of elution buffer was added and incubated at 98C for 10 min to elute bead-bound DNA. DAP-seq libraries were constructed for sequencing.