16S rRNA V3 amplicon of apple orchard soil microcosm, Ctrl, replicate R1
16S rRNA gene V3 hypervariable region amplified from total DNA extracted from apple orchard soil laboratory microcosms exposed to zinc oxide nanoparticles. Amplification used universal primers 338F (5'-ACTCCTACGGGAGGCAGCAG-3') and 533R (5'-TTACCGCGGCTGCTGGCAC-3'). Sequenced on an Illumina MiniSeq using a 300-cycle kit (2 x 150 bp paired-end) under standard conditions.