Amplicon sequencing of Mediterranean short-term sustainable grazing 5
DNA was extracted from soil using the DNeasy PowerSoil Pro Kit (Qiagen). For prokaryotic library preparation, a fragment of the V4 region of the bacterial 16S rRNA gene was amplified using the primers 515F (5GTG YCA GCM GCC GCG GTA A 3) (modified from Parada et al. (2016) and 806R (5GGA CTA CNV GGG TWT CTA AT 3) (modified from Apprill et al. (2015). For fugal library prep, a fragment of the ITS1 region was amplified with the primers ITS1f (5 CTT GGT CAT TTA GAG GAA GTA A 3) (modified from White et al., 1990) and ITS2 (5 GCT GCG TTC TTC ATC GAT GC 3) (White et al., 1990).