WGS of Acinetobacter baumannii strain ALF25E195
DNA was extracted from liquid culture using the GenFind V3 Reagent Kit (Beckman Coulter). Libraries for long-read sequencing were prepared using the Ligation Sequencing Kit with Native Barcoding Expansion (Oxford Nanopore Technologies) and sequenced on the MinION instrument with an R10.4.1 flow cell (Oxford Nanopore Technologies) for 48 hours. Basecalling was performed with Dorado v0.5.1 using the super basecalling model.