WT plants under drought stress 2
Raw sequencing data was first filtered by fastp (v0.23.2), low-quality reads were discarded and the reads with adaptor sequences were trimmed. UMI sequences were identified and extracted from clean reads using umikit (v1.0) based on anchor sequences; paired reads possessing both valid 5 and 3 UMI anchors were classified as valid UMI reads. Valid UMI reads with insert lengths shorter than 15 nt were discarded.