16S/ITS amplicon sequencing of Tris buffer negative control (Col-0-myb28-29-Tris_control_2)
Dual-indexed amplicon libraries (341F/799R bacterial 16S V3-V4, ITS and 18S primers); paired-end 2x300 MiSeq; demultiplexed on dual barcodes with cutadapt; primers retained in submitted reads