RNA-seq of homo sapiens: cell culture
RNA was isolated from human 293FT cells stably expressing one of five NOTCH1 constructs: empty vector control (EF1_Vec), wild-type NOTCH1, the frameshift mutant P2469fs, the in-frame duplication P1582_L1585dup, or the compound double mutant P2469fs + P1582_L1585dup. Each condition was prepared in three biological replicates (n=15 libraries total). Poly(A)-selected paired-end RNA-seq libraries (SI_43798SI_43812) were sequenced on an Illumina NovaSeq 6000 instrument (flowcell HHHKGDRX7), generating paired-end FASTQ files. Raw sequencing data are publicly available under the library identifiers listed above.