Mus musculus ear skin bulk RNA-seq, high-fat diet (HFD), recovery phase after IMQ-induced inflammation, replicate 4
Total RNA was extracted from frozen mouse ear skin tissue. RNA samples were treated with DNase to remove residual genomic DNA. Ribosomal RNA was depleted prior to library construction. Sequencing libraries were prepared using a stranded RNA-seq library preparation protocol and sequenced on an Illumina platform with 2  150 bp paired-end reads, targeting approximately 50 million reads per sample.