16S/ITS amplicon sequencing of Arabidopsis thaliana Col-0 myb28/29 root/leaf microbiome, PDB, NIL
Dual-indexed amplicon libraries (341F/799R bacterial 16S V3-V4, ITS and 18S primers); paired-end 2x300 MiSeq; demultiplexed on dual barcodes with cutadapt; locus primers trimmed with cutadapt (-e 0.2 -O 15 -n 2, 5-prime anchored)