Paired-end mitochondrial DNA sequencing of 2306277037
The gas-liquid system of the instrument first pumps the DNA nanoballs (DNB) into the patterned array substrate and fixes them there. Then, the sequencing templates and sequencing reagents are pumped in. After the pumping, the sequencing templates hybridize with the junctions of the DNBs on the substrate in a complementary manner. Under the catalysis of the DNA polymerase, the sequencing templates combine with the fluorescently labeled probes in the sequencing reagents. Next, by exciting the emission of light from the fluorescent groups, the light signals emitted by different fluorescent groups are captured by the instrument camera. After processing, they can be converted into digital signals, which are then transmitted to the computer for further processing and ultimately obtain the base sequence information of the sample to be tested.