RNA amplicon of HEK293T: overexpressing RaA3Z1a and Marburg viral fragments
Total RNA was extracted from HEK293T cells expressing the indicated bat APOBEC3 construct and synthetic Marburg viral fragments. RNA was reverse-transcribed into cDNA, followed by PCR amplification of the target region and paired-end Illumina amplicon sequencing to quantify APOBEC3-mediated C-to-U RNA editing.