16S rRNA amplicon sequencing of soil metagenome: Soil_bacteria_14
Soil microbial community DNA was extracted using E.Z.N.A. Soil DNA Kit. The bacterial 16S rRNA V3-V4 region was amplified with primers 338F (ACTCCTACGGGAGGCAGCAG) and 806R (GGACTACHVGGGTWTCTAAT). PCR products were purified, pooled in equimolar amounts, prepared with NEXTFLEX Rapid DNA-Seq Kit, and paired-end sequenced on an Illumina NextSeq 2000 platform by Majorbio Bio-Pharm Technology Co. Ltd.