WGS of homo sapiens: pregnancy vaginal_swab
Genomic DNA for NGS library preparation and sequencing. Libraries were prepared using the Takara Bio ThruPLEX DNA-Seq kit and DNA Unique Dual Index kit. Completed libraries were QCd and quantified using a combination of Qubit dsDNA HS and Agilent 4200 TapeStation HS DNA1000 assays. All libraries were diluted to a normalized concentration of 10nM. The libraries were divided into pools for multiplexed sequencing. Pools were quantified using the Invitrogen Collibri Quantification qPCR kit. Each pool was loaded onto one (1) lane of an Illumina NovaSeq S4 flow cell and sequencing was performed in a 2x150bp paired end format using a NovaSeq 6000 v1.5 300 cycle reagent kit. Base calling was done by Illumina Real Time Analysis (RTA) v3.4.4 and output of RTA was demultiplexed and converted to FastQ format with Illumina Bcl2fastq v2.20.0.