ITS amplicon sequencing of soil metagenome: Soil_fungus_02
Soil microbial community DNA was extracted using E.Z.N.A. Soil DNA Kit. The fungal ITS marker region was amplified from soil DNA. PCR products were purified, pooled in equimolar amounts, prepared with NEXTFLEX Rapid DNA-Seq Kit, and paired-end sequenced on an Illumina NextSeq 2000 platform by Majorbio Bio-Pharm Technology Co. Ltd.