Bisulphite converted PEG 1
DNA from the rapeseed was isolated using DNA extraction kit Nucleon PhytoPure (Merck). Bisulphite conversion of DNA was carried using the kit EZ DNA Methylation-Gold Kit (Zymoresearch, D5005). Bisulphite-converted DNA was used as a template for the targeted DNA amplification. PCR fragments were purified and used for sequencing.