Male_fecal_metagenome_sample
A total of 0.2 g DNA per sample was used as input material for the DNA library preparations. Initially, the genomic DNA sample was fragmented by sonication to reach a size of 350 bp. Next, the DNA fragments underwent end polishing, A-tailing, and ligation with the full-length adapter for sequencing, followed by PCR amplification. The PCR products were then purified using the AMPure XP system (Beverly, USA). Subsequently, the library quality was assessed on the Agilent 5400 system (Agilent, USA) and quantified using QPCR (1.5 nM). After conducting library quality control, the different libraries were pooled based on their effective concentration and the desired data amount. The 5-end of each library was phosphorylated and cyclized. Then, loop amplification was performed to generate DNA nanoballs. Finally, these DNA nanoballs were loaded into a flow cell with DNBSEQ-T61 for sequencing at Novogene Bioinformatics Technology Co., Ltd (Beijing, China).