RNA-Seq of rice shoot
Total RNA was extracted from two-leaf-one-heart stage seedlings of wild-type (Dongnong 427) and osmybas1 mutants exposure to 120 mM NaCl for 0 and 12 h using the TRIzol reagent kit. Three independent biological replicates were analyzed per treatment group. Poly(A) mRNA was enriched via oligo(dT) magnetic beads and fragmented to construct cDNA libraries. Sequencing was executed on an Illumina NovaSeqTM 6000 platform. Transcript abundance was quantified as Fragments Per Kilobase of transcript per Million mapped reads (FPKM). Differentially expressed genes (DEGs) were identified using the R package edgeR (v4.2.3) with thresholds of false discovery rate (FDR) <0.05 and |Log2 (Fold Change)|  1.