16S rRNA V3 amplicon of apple orchard soil microcosm, qr_250, replicate R1
16S rRNA gene V3 hypervariable region amplified from total DNA extracted from apple orchard soil laboratory microcosms exposed to zinc oxide nanoparticles. Amplification used universal primers 338F (5'-ACTCCTACGGGAGGCAGCAG-3') and 533R (5'-TTACCGCGGCTGCTGGCAC-3'). Sequenced on an Illumina MiniSeq using a 300-cycle kit (2 x 150 bp paired-end) under standard conditions.