16S rRNA gene amplicon sequencing of mouse fecal
Microbial community DNA was extracted from mouse fecal samples. The V3V4 hypervariable region of the bacterial 16S rRNA gene was amplified using primers 338F and 806R with barcode sequences. The purified amplicons were used for library construction and sequenced on an Illumina NextSeq 2000 platform using paired-end sequencing.