Metagenomic sequencing of Winogradsky Column Shallow Subsurface
DNA extractions were performed using the ZymoBIOMICS DNA Miniprep kit, following the protocol with the following changes: 1) Lysis was performed using the Vortex Genie for 40 minutes of continuous bead beating. 2) Eluted DNA was not run through the Zymo-Spin III-HRC Filter. Library preparation was performed using the Illumina DNA Prep tagmentation kit with Illumina Unique Dual Indexes.