spleen DNA
Transcriptomic analysis of the spleens of Pargo-UNAM and O. niloticus challenged with A. veronii, as well as controls, at 48 hours post-infection. The spleen was fixed in DNA/RNA Shield and maintained at -70C. The TRIzol method was employed to extract RNA. The integrity was verified by 1% agarose gel electrophoresis and the RIN number was determined using Agilent Bioanalyzer 2100 system. Samples with RINs greater than 8 were sequenced. Two biological replicates of each treatment per tilapia variety were sequenced in a 2x150 paired-end read format using a NovaSeq 6000 sequencing platform.