Oxford Nanopore MinION sequencing of Fusarium poae strain IBT 9924
Genomic DNA was extracted from fungal tissue using the Illustra Phytopure Genomic DNA Extraction kit (GE Healthcare). High-molecular-weight DNA was size-selected to enrich for fragments in the 1040 kb range using a SageELF instrument (Sage Science Inc), and fractions with the highest abundance were combined. Sequencing libraries were prepared with an Oxford Nanopore ligation kit following the manufacturers instructions, with minor protocol adjustments to optimize DNA recovery and long-read output. The library was loaded onto a FLO-MIN106 flow cell and sequenced on a MinION device for 48 hours.  Reads were basecalled using Guppy v4.2.2.