16S rRNA gene amplicon sequencing of freshwater microplastic-associated biofilm
Microbial biofilm was collected from the surface of microplastics after in situ incubation in freshwater. Total community DNA was extracted, and the V3V4 region of the bacterial 16S rRNA gene was amplified using primers 338F and 806R. Amplicon libraries were sequenced on an Illumina MiSeq platform using paired-end 308 bp sequencing.