Amplicon Sequencing of Wetlands Water Samples Region B
eDNA was extracted from water sample using the DNeasy PowerWater Kit method and purified using AMPure XP beads,, indices were added via the Nextera XT Index Kit v2 and quantified using HeliXyte Green. Sequencing performed on Illumina MiSeq platform and bioinformatics were conducted using DADA2 and R Studio.