Sequence capture of UCEs for Stygnus n. sp.
Genomic DNA was extracted using either the Qiagen DNEasy Blood and Tissue kit or a silica-bead-based protocol depending on sample preservation. DNA was fragmented to a target average length of 500 bp via enzymatic fragmentation. Indexed libraries were constructed using Kapa HyperPlus or Hyper Prep Kits (KAPA Biosystems) and hybridized using the 'Opiliones' UCE probe set. Sequencing was performed on an Illumina NovaSeq S4 platform (216bp paired-end).