Illumina sequencing of Fusarium poae strain DAOMC 215452
Mycelium was frozen at -80 °C, lyophilized, and pulverized via bead beating. Genomic DNA was purified from ground mycelium with a Zymobiomics MagBead DNA/RNA (Zymo Research Corporation, Irvine, CA, USA). For preparation of sequencing libraries, ~100 ng of total genomic DNA were used as the template and processed using the KAPA HyperPlus Kit for PCR-free workflows (Roche, Switzerland) followed by eight rounds of amplification to increase library yields. Sequencing libraries were pooled and size selected for 350-900 bp fragments using a combination of Zymo Select-a-size Clean & Concentrator and gel purification. Whole genomes were sequenced on a NovaSeq Sequencing System (Illumina, San Diego, CA, USA). Reads were subsequently trimmed using Trimmomatic 0.39 for quality using -PHRED33 and to trim illumina adapters using TRAILING:20 and MINLEN:50.