77_days_post_infection_dln_3
Lesions and corresponding draining lymph nodes (dLNs) from BALB/C mice were sampled at various time points (2h, 6h, 48h, 6 days, 14 days, 31 days, and 77 days) post-infection to extract total RNA. Total RNA isolation from lesions was carried out using the RNeasy Mini kit (Qiagen), followed by rRNA depletion using the Eukaryote RiboMinus kit (Invitrogen, Carlsbad, CA). Subsequently, RNA-seq libraries were prepared utilizing the Illumina TruSeq Stranded kit (Illumina, San Diego, CA). The libraries were sequenced on an Illumina NextSeq500 instrument (Illumina) using the Illumina NextSeq500 High Output kit. For total RNA isolation from dLNs, a RNeasy Micro Kit (QIAGEN) was employed, and sequencing libraries were constructed using the TruSeq Stranded mRNA library Prep. Finally, the libraries were sequenced on an Illumina HiSeq2500 platform.