RAN-seq of HCA-7 cells: PGE2 stimulation, 6 hr
Total RNA was extracted using TRIzol reagent (Thermo Fisher Scientific), and mRNA was purified using the NEBNext Poly (A) mRNA Magnetic Isolation Module (New England Biolab, Ipswich, MA, USA). DNA libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolab), and RNA sequencing was performed on a HiSeq 2000 platform (Illumina, San Diego, CA, USA)