RNAseq of WT Caulobacter crescentus NA1000 pilAT36C
complementary DNA (cDNA) was synthesized from mRNA using random primers and reverse transcriptase. The second DNA strand was then generated to form double-stranded DNA (dsDNA) from the mRNA template. This dsDNA was used for library preparation with the Nextera XT DNA Library Prep Kit (Illumina)