RNA-seq of Dasineura rozhkovi: female imago full body
Three (for males) and five (for females) independent biological replicates were obtained by RNA22extraction from whole insects (1620 males or females per sample). The total RNA was extracted23using TRI Reagent (-9424, Sigma, USA) according to the manufacturers instructions.245The quality of the RNA samples was evaluated using an Agilent Bioanalyzer 2100 (Agilent, Santa1Clara, CA, USA). All the samples had RIN of 7.5 or higher. RNAseq library preparations were2carried out with 1 mkg of total RNA using a TruSeq Stranded mRNA Library Prep Kit and TruSeq3RNA UD Indexes (Illimina, San Diego, CA, USA) according to the manufacturers instructions4for barcoded libraries with minor modifications (6 min RNA fragmentation time and 10 PCR5cycles were used). After normalization, the barcoded libraries were pooled and sequenced on a6GenolabM sequencer (GeneMind, Shenzhen, China) 2  150 bp using GenoLab M Sequencing7Set V4.0 (FCH 300 cycles)