RNA-seq of FACS-sorted Sox10-Venus-positive cells from pooled L3-L4 DRGs, right contralateral unoperated side, week 3, biological replicate 2
Biological replicate 2. L3 and L4 dorsal root ganglia from the right side, corresponding to the contralateral unoperated side of the same SNI mice, were collected from 3 Sox10-Venus transgenic female mice and pooled. Samples were collected 3 weeks after left-sided spared nerve injury. After pooling the DRGs, Venus-positive cells were isolated by fluorescence-activated cell sorting (FACS). Total RNA was used for low-input, non-stranded RNA-seq library preparation with the SMART-Seq HT PLUS kit (Takara Bio; Cat. No. R400749). Full-length cDNA was generated by the SMART method, amplified, fragmented, adapter-ligated, and indexed. Libraries were sequenced on an Illumina NovaSeq 6000 with 150-bp paired-end reads (PE150).