metagenomics of black soil sample T96
DNA was extracted using FastDNA Spin Kit for Soil (MoBio Laboratories, Inc., Carlsbad, CA, USA) following the manufacturers protocol. Approximately 500 ng of DNA per sample was isolated for metagenomic sequencing on the Illumina NovaSeq (NovaSeq 6000 S4 Reagent Kit v1.5, 300 cycles) at Majorbio Bio-Pharm Technology Co., Ltd. (Shanghai, China)