CinC-seq of homo sapiens liver tumor
After fragmentation and library preparation using xGen cfDNA & FFPE DNA Library Preparation Kit (IDT, Coralville, Iowa), short DNA/cDNA fragments were enriched using custom hybrid capture probes (IDT, Coralville, Iowa/GOAL consortium) and sequenced using a 2 x 150bp sequencing chemistry on an Illumina 2000. Clinically validated CinCSeq NGS analysis evaluates single nucleotide variants (SNV), multi-nucleotide variants (MNV), and insertions and deletions (indels) in approximately 95% of the DNA coding exon sequences of 383 cancer-related genes, copy number variants (CNV) in 531 cancer-related genes, and gene fusions/structural variants in 183 cancer-related RNA genes.