HG002_ONT_WGS
Sequencing libraries were prepared utilizing the ONT Ligation Sequencing Kit V14 (Cat. no. SQK-LSK114). DNA damage repair and end-preparation were performed using the NEBNext FFPE DNA Repair Mix (New England Biolabs, Cat. no. M6630) and the NEBNext Ultra II End Repair/dA-Tailing Module (New England Biolabs, Cat. no. E7546). The final prepared libraries were loaded onto PromethION R10.4.1 (ONT, Cat. no. FLO-PRO114M) flow cells and sequenced on a Nanopore PromethION P48 platform.