Whole-genome sequencing of T9
Genomic DNA was fragmented by sonication to approximately 350 bp. Sequencing libraries were prepared using the NEBNext Ultra DNA Library Prep Kit, followed by end-polishing, A-tailing, adapter ligation, PCR amplification, purification, and library quality assessment. The libraries were circularized and amplified by rolling-circle amplification to generate DNA nanoballs (DNBs), followed by paired-end sequencing on the DNBSEQ-T7 platform.