Topo-Seq, ChIP-Seq of S. pneumoniae MXIP6_R1: MXF 512xMIC
DNA fragments of  200 to 700 bp were selected after repairing ends, adenylating 3 ends, and ligating indexed paired-end adapters. The selected DNA fragments were enriched by PCR with the PCR primer cocktail that anneals to the ends of the adapters. Quality control of the library was determined with a 2100 Bioanalyzer Instrument (Agilent Technologies)