PacBio HiFi whole-genome sequencing of Cardamine anhuiensis: leaf tissue
High-molecular-weight genomic DNA was extracted from fresh leaf tissue of Cardamine anhuiensis. DNA quality was detected by agarose gel electrophoresis, and concentration was quantified with Qubit fluorometer. Qualified gDNA was fragmented, end-repaired, A-tailed, and ligated with PacBio hairpin adapters to construct SMRTbell library; BluePippin system was used for fragment size selection. The validated SMRTbell library was sequenced on PacBio Revio platform. Raw polymerase reads were converted to CCS reads, and reads with QV  20 were retained as final HiFi data via Google DeepConsensus algorithm.