embryo2
mRNA was purified and enriched using SPRI magnetic beads, followed by high-temperature fragmentation. Double-stranded (ds) cDNA was then synthesized via a two-step reverse transcription reaction. The purified ds cDNA was sequentially subjected to end repair, A-tailing, and adapter ligation, and finally amplified by polymerase chain reaction (PCR). Strand-specific libraries for sequencing were purified using AHTS DNA Clean Beads. The libraries were sequenced on the Illumina NovaSeq X Plus platform by Gene Denovo Biotechnology Co. (Guangzhou, China) with PE150 reads