16S rRNA V3-V4 amplicon sequencing of rice shoot endosphere bacterial community, treatment level 0.1, biological replicate 1
Genomic DNA from rice endosphere tissue was used to amplify the bacterial 16S rRNA gene V3-V4 region with barcoded 341F/806R-family primers. Amplicons were gel purified, quantified, pooled in equimolar proportions, and prepared with the NEBNext Ultra II DNA Library Prep Kit. Libraries were sequenced as paired-end reads on an Illumina platform. The submitted files are demultiplexed raw FASTQ pairs that retain sample barcode and primer sequences.