Single-cell RNA-seq of human PBMCs from patient-coded sample P3, collected before IRE
Single-cell 3' RNA-seq library prepared from human peripheral blood mononuclear cells isolated by Ficoll-Paque PLUS density-gradient centrifugation from a patient with clinically localized prostate cancer, collected before IRE. Libraries were prepared using the 10x Genomics Chromium Single Cell 3' Gene Expression v3 workflow (Chromium Single Cell 3' Library and Gel Bead Kit v3, PN-1000075). Sequencing reads comprised a 26-bp Read 1 containing the cell barcode and UMI and a 100-bp Read 2 containing transcript-derived sequence. Patient-coded sample identifier: P3_PRE.