PacBio HiFi WGS of Platythyrea mocquerysi: PB131
High-molecular-weight genomic DNA from two live pupae of Platythyrea mocquerysi was extracted with the Qiagen Gentra Puregene Tissue Kit. HMW gDNA was sheared with a Megaruptor 3 and SMRTbell libraries were prepared using SMRTbell prep kit 3.0, including end-repair/A-tailing, ligation of barcoded overhang adapters, AMPure PB purification, and nuclease treatment. Two pooled barcoded libraries were sequenced on a PacBio Sequel IIe in September 2023 at the Next Generation Sequencing Platform, University of Bern, generating HiFi circular consensus reads.