RNA-Seq of Salmonella Typhimurium 14E1050 donor treated with 0.5 mM benzoic acid, replicate 1
Transcriptome profiling of Salmonella Typhimurium 14E1050 (donor strain) under benzoic acid stress. Total RNA was extracted from in vitro pure cultures, and cDNA libraries were constructed using random priming. Paired-end 150 bp sequencing was performed on the Illumina platform. Raw reads were filtered with Fastp (removing adapter sequences and reads with mean quality < Q20), aligned to the reference genome with Bowtie2, and gene expression was quantified with HTSeq.