RNA-Seq of Homo sapiens: KYSE70 ESCC cells + HOXC9 siRNA
KYSE70 human esophageal squamous cell carcinoma cells were transfected with HOXC9-targeting siRNA and cultured for 24-48 h. Approximately 1x10^7 cells were harvested and lysed in TRIzol(R) reagent (Life Technologies) for total RNA extraction. PolyA-selected mRNA sequencing libraries were constructed and sequenced as 150 bp paired-end reads on an Illumina NovaSeq 6000 platform by Novogene Bioinformatics Technology Co., Ltd. (Beijing, China).