RNA-Seq of Pontastacus leptodactylus hepatopancreas control replicate 2
Total RNA was extracted from the hepatopancreas of Pontastacus leptodactylus in the control group. Polyadenylated mRNA was enriched using oligo(dT) magnetic beads, fragmented, and reverse-transcribed into cDNA. After end repair, A-tailing, adapter ligation, size selection, and PCR amplification, the library was sequenced as 150-bp paired-end reads.