DNA-Seq (HiFi) of Sorbus medogensis: mature tree leaves
High-molecular-weight genomic DNA will be extracted from the plant tissue using a standard CTAB method and further purified with Qiagen Genomic-tip columns. The library will be constructed using the PacBio SMRTbell Express Prep Kit 3.0. Approximately 5 g of gDNA will be sheared to 15-20 kb fragments. After end-repair and hairpin adapter ligation, the library will undergo size selection using BluePippin (>15 kb) and quality-checked on a Fragment Analyzer. Sequencing will be performed on the PacBio Revio platform with 30-hour movie time. HiFi reads will be generated using the CCS algorithm in SMRT Link software.