Long-read shotgun metagenomic sequencing of hospital wastewater: eDNA
Long-read sequencing libraries were prepared using the Ligation Sequencing Kit (Oxford Nanopore Technologies, Oxford, UK), according to the manufacturer's instructions. Library amplification was performed using Hot Start LongAmp Taq 2 Master Mix (New England Biolabs, Ipswich, MA, USA). The libraries were loaded onto an R10.4.1 flow cell and sequenced on a MinION Mk1B device (Oxford Nanopore Technologies).