RNA-seq sequencing
Total RNA was extracted from ovary of Petenia splendida using TRIzol reagent and treated with DNase I to remove genomic DNA contamination. Polyadenylated mRNA was enriched using oligo-dT magnetic beads, and stranded cDNA libraries were constructed with a strand-specific RNA library preparation kit. The libraries were sequencedon a BGISEQ MGISEQ-2000RS platform to generate 2150 bp paired-end reads for transcriptome analysis.