RNA-Seq of Cardamine anhuiensis: leaf tissue
Total RNA was extracted from leaf tissue of Cardamine anhuiensis. Poly(A)+ mRNA was enriched using oligo(dT) magnetic beads, fragmented and reverse-transcribed into double-stranded cDNA with random hexamer primers. The sequencing library was prepared via end repair, A-tailing, adapter ligation, size selection and PCR enrichment, and then sequenced on the Illumina NovaSeq platform with a paired-end 150 bp (PE150) strategy.