Direct RNA sequencing of HTLV-1 transcripts in control MT-2 cells with ALKBH5 treatment
Total RNA was extracted from control MT-2 cells harboring integrated HTLV-1 proviruses. The RNA was divided into mock-treated and recombinant ALKBH5-treated samples, followed by Oxford Nanopore direct RNA sequencing to characterize HTLV-1 transcript profiles and RNA modification signals before and after ALKBH5-mediated demethylation.