Blank control group 3
The feces gDNA samples were purified using the Zymo BIOMICS DNA Miniprep Kit, followed by full-length 16S rDNA gene amplification via PCR with universal primers 8F (5-AGAGTTTGATCATGGCTCAG-3) and 1492R (5-CGGTTACCTTGTTACGACTT-3). The PCR products were subsequently analyzed by agarose gel electrophoresis, purified, and quantified for downstream applications. High-throughput sequencing was conducted on the Nanopore GridION sequencer.