WGS of Solanum tuberosum cv. Desiree control
The WGS was performed from minimum 100 ng of DNA having OD260/280 ratio of 1.8-2.0. The library (350 bp) was prepared as follows. The potato genomic DNA was fragmented and the fragments were then end-polished, A-tailed, and ligated with adapters before further size selection. PCR amplification was then performed and the PCR-products were purified using the AMPure XP system (Beckman Coulter). The resulting library was assessed on the AgilentFragment Analyzer System (Agilent) and quantified to 1.5 nM through Qubit (ThermoFisher Scientific) and qPCR. The qualified libraries were pooled and sequenced on Illumina NovaSeq X Plus (150 bp, pair-end read) with an average 30 sequencing depth.