PacBio HiFi WGS of Stigmatomma oregonense: PB140
High-molecular-weight genomic DNA from three late-stage larvae of Stigmatomma oregonense (guts removed before extraction) was extracted with the Qiagen Gentra Puregene Tissue Kit. Because DNA input was limited (~20 ng), the PacBio Ultra-Low DNA Input protocol was used. HMW gDNA was sheared with a Megaruptor 3 and SMRTbell libraries were prepared using SMRTbell prep kit 3.0. Three pooled libraries were sequenced on a PacBio Revio in November 2023 at the Next Generation Sequencing Platform, University of Bern, generating HiFi circular consensus reads.