16S rRNA gene amplicon sequencing of Bacillus subtilis BUUC2502 from shrimp pond sediment
Full-length 16S rRNA gene amplified by PCR from genomic DNA of the bacterial isolate; 5 uL of the purified PCR product was fragmented with S-TN5 transposase (Tsingke) at 55 C for 10 min and libraries were prepared with S-1.2x-TN5 library PCR mix, size-selected by agarose gel extraction, and sequenced 2x151 bp on an Illumina MiSeq using the MiSeq Reagent Micro Kit v2 (U2Bio FastNGS service).