Bacillus pumilus
Genomic DNA was extracted using the Zymo Quick-DNA (Fungal/bacterial) mini prep kit following the manufacturer's protocol. Libraries were prepared from 1 ng input DNA using the Nextera XT kit (Illumina), involving transposase-mediated fragmentation and PCR addition of dual indexes. Library quality and fragment distribution were confirmed using an Agilent 2100 Bioanalyzer, normalized, pooled, cleaned with 0.7 AMPure XP beads, and quantified using the Qubit dsDNA HS assay. Paired end (2  150 bp) sequencing was performed on the AVITI platform (Element Biosciences) following standard operating protocols, with final pooled libraries denatured, diluted, and loaded according to manufacturer guidelines for optimal cluster density and data quality.