Wild-type C57BL/6J mouse splenocytes stimulated with LPS 500 ng/ml for 5 hours
Transcriptome analysis was performed on transgenic IL-37a mice (transgenic mice with CMV promoter) splenocytes. Splenocytes of WT C57BL6/J and IL-37aTg mice were seeded in 24 wells cell culture plate in the density of 1106/ml. These cells were stimulated with 500 ng/ml LPS for 5 hours. Total RNA from each sample was isolated and purified. Construction of cDNA libraries and next-generation sequencing was performed.