Direct RNA sequencing of HTLV-1 Transcripts in MT-2 Cells
Total RNA was extracted from HTLV-1-infected MT-2 cell cultures using TRIzol reagent (Invitrogen). Polyadenylated RNA molecules were ligated to sequencing adapters without reverse transcription or PCR amplification using the Oxford Nanopore Direct RNA Sequencing Kit (SQK-RNA004), and sequenced on a MinION with R10.4.1 flow cells.