Whole genome of Pseudomonas sp. BH
High-molecular-weight genomic DNA was extracted from pure bacterial cells using the CTAB (cetyltrimethylammonium bromide) method, followed by RNase A treatment. DNA purity (OD260/280 ratio > 1.8) and integrity were confirmed via Nanodrop and pulsed-field gel electrophoresis (PFGE). A total of 1.5 g of sheared DNA (Covaris M220, target insert size ~450 bp) was processed with the Illumina TruSeq DNA PCR-Free Library Preparation Kit. The workflow included enzymatic end-repair, 3'-end adenylation, and ligation to indexed Y-shaped adapters. Ligation products were purified twice using AMPure XP beads (0.6 and 0.2 sequential double-size selection) to precisely retain fragments around 450500 bp. Importantly, no PCR amplification step was performed after adapter ligation to minimize GC bias. The final library was quantified by Qubit HS assay and sized by Bioanalyzer 2100 (Agilent). Sequencing was performed on the Illumina NovaSeq 6000 using the 2  150 bp paired-end mode.