GSM9957050: M4_A; Homo sapiens; RNA-Seq
Tumor samples extracted fro the mice were homogenized in Trizol reagent and RNA was isolated and treated with DNase. Libraries were prepared with Ultra II Directional RNA Library Prep Kit for Illumina using 500 ng of RNA input. Final libraries were sequenced on a NextSeq 2000 instrument using NextSeq 1000/2000 P2 Reagents with 122 bp for read 1 and 8 bp dual-index sequencing.