4_NEUFR0148 R1
Single-nucleus RNA-seq and ATAC-seq libraries were prepared by the Harvard Single Cell Core using the Chromium Next GEM Single Cell Multiome ATAC + Gene Expression User Guide (10x Genomics, Rev F). Nuclei were processed on the 10x Chromium Controller with the Chromium Next GEM Chip J. Samples underwent Transposition, GEM Generation and Barcoding, Post-GEM Incubation Cleanup, Pre-Amplification PCR, ATAC library construction, cDNA amplification, and Gene Expression library construction per manufacturer's protocol. Libraries were quality-checked using the Agilent Bioanalyzer and sequenced on the Illumina NovaSeq X platform by the Molecular Biology Core Facilities at Dana-Farber Cancer Institute.