GSM9821961: VL-51 cells 24h MLT_ replicate 4; Homo sapiens; RNA-Seq
Total RNA was extracted with RNeasy Plus Mini Kit according to the manufacturer's protocol (Qiagen, cat. no. 74134) RNA quality was assessed on a Fragment Analyzer (Agilent Technologies). The RNAs had  RQNs between 9.4 and 10. RNA-seq libraries were prepared from 250 ng of total RNA with the Watchmaker mRNA Library Prep Kit (Watchmaker Genomics) using a unique dual indexing strategy, and following the official protocol. Libraries were quantified by a fluorometric method (QubIT, Life Technologies) and their quality assessed on a Fragment Analyzer (Agilent Technologies). Sequencing was performed on an Element Biosciences Aviti with a 2x75 cycles sequencing kit Cloudbreak FS High Output (run as single read 150).  Base calling and demultiplexing was done with bases2fastq version: 2.0 with filter mask R1:N15Y15N*.