GSM9754600: CD3e+CD8+ T cells in Py8119 tumors from WT mice; Mus musculus; ATAC-seq
Target cells were sorted from live cells with CD45+CD3e+CD8+. 50,000–100,000 cells per sample were used for nuclei isolation, during which cells were lysed on ice for 3 minutes. Permeabilized nuclei were subjected to transposition using TDE1 transposase (Illumina, CA) at 37°C for 60 minutes, followed by purification with the Zymo DNA Clean and Concentrator-5 kit. ATAC-seq library was prepared with Nextera DNA library preparation kit (Illumina)