WGS of S.aureus mutants
3 g of gDNA each sample was processed with 0.5X AMPure XP beads for gDNA clean-up and buffer exchange, and then the purified gDNA was processed for DNA fragmentation using Covaris g-TUBE device. After DNA QC, 500 ng of fragmented DNA each sample was used to generate SMRTbell library and then BluePippin size selection for obtaining the library with insert size >6Kb, followed by beads clean-up and library pooling. The SMRTbell library pool was then sequenced on the PacBio Sequel system. For sequencing preparation, primer annealing and polymerase binding were performed based on binding calculator instructed by PacBio.