16S rRNA V3-V4 amplicon, rhizosphere of green cos lettuce, anaerobic digestion effluent at EC 1.5 mS/cm, day 31
16S rRNA gene amplicon library targeting the V3-V4 hypervariable region, amplified from rhizosphere-associated root tissue DNA extracted by a CTAB-based method. Primers 338F/806R. PCR products were purified, quantified and normalised before library construction and paired-end sequencing (2 x 250 bp). Submitted reads are quality-filtered: Trimmomatic v0.33 (50 bp sliding window, mean Q20) and primer removal with Cutadapt v1.9.1 (max 20% mismatch, min 15 bp overlap).