GSM8751010: EdU_seq_DMSO1h; Mus musculus; OTHER
EdU-seq was performed by following the protocol in (Petryk et al., 2021) without the chromatin immunoprecipitation steps. Briefly, 100 μl DNA in TE buffer was decrosslinked overnight by incubation for 30 minutes with 5 μl of RNase A (10mg/ml) at 37ºC followed by addition of 1 μl proteinase K (10mg/ml), 2.5 μl of 20% SDS and 2 μl of 5M NaCl and incubation for 10 hours at 37ºC followed by 6 hours at 65ºC. DNA was then purified by MinElute and quantified by Qubit. Libraries were prepared with KAPA hyperprep kit (Roche, 07962363001) according to manufacturer instructions and sequenced single-end on a NextSeq 2000