Kas-NT
ac4C RIP-seq:mRNA was randomly fragmented into 100200 nt RNA fragments, and incubated with protein A/G magnetic beads pre-conjugated to anti-ac4C antibody or anti-IgG antibody to obtain immunoprecipitated RNA fragments.The libraries were performed on an Illumina NovaSeq 6000 platform with 150-bp, paired-end reads. All raw sequencing data were processed through a standardized computational pipeline, including quality filtering, adapter trimming, alignment to the reference genome, and peak calling, to identify ac4C-enriched regions and quantify differential ac4C-modified transcripts