single nucleus RNAseq of liver samples
single nucleus RNAseq of liver samples
Mice were euthanized ad libitum, anesthetized with isoflurane and sacrificed by decapitation. Livers were dissected, weighed, snap-frozen and stored in liquid nitrogen and shipped on dry ice. A R26LSL-tdTom B Lamtor2flox/flox;R26LSL-tdTom C Lamtor2flox/flox;Albumin-Cre D Lamtor2flox/flox;R26LSL-tdTom;Albumin-Cre Treatment: Groups A and B received AAV8-TBG-Cre (2 × 10^11 GC/mouse) intraperitoneally 4 weeks before harvest; Groups C and D had no AAV treatment. Number of replicates: We generated 9 snRNA-seq libraries (biological samples) from 15 mice total across four groups (A–D). Libraries per group and mice per library: A1 (1), A2 (1); B1 (2), B2 (2), B3 (1); C1 (3), C2 (3); D1 (1), D2 (1). Some libraries were prepared from pooled tissue (2–3 mice). the standard 10x 3' gene expression protocol was followed the standard 10x 3' gene expression protocol was followed