GSM9883397: SUHW_SUHW.HA_E1620_rep2; Drosophila melanogaster; ChIP-Seq
Chromatin was sonicated using a Covaris instrument (Diagenode) for 10 min in 130 µl tubes (peak power = 105W; duty factor = 10%; cycles per burst = 200). Antibodies against HA, PC (SCHUETTENGRUBER et al. 2009), Su(HW) (MOSHKOVICH AND LEI 2010)  and SU(Z)12 (LOUBIERE et al. 2017) were used for immunoprecipitation at a dilution of 1:100. Following decrosslinking, DNA was purified using MicroChIP DiaPure columns (Diagenode). DNA libraries for sequencing were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina. Paired-end sequencing (sequencing (150 bp; approximately 2 Gb per sample) was performed by Novogene (https://en.novogene.com/). All experiments were performed in biological duplicates.