GSM9049371: Multiome, Heart, Malonate Sham, RNA; Mus musculus; RNA-Seq
Heart tissue below the ligation was collected below the ligation and snap frozen in liquid nitrogen. The tissue was then homogenized using lysis buffer and pulverization using a plastic pestle at 4C. The cells were filtered through a spin column to isolate nuclei from cells using the 10x Chromium Nuclei isolation kit (10xGenomics, PN-1000493). Library construction was performed using the manufacturer's protocols for the Chromium Next GEM Single Cell Multiome ATAC + Gene Expression kit (10xGenomics, PN-1000283) by the University of Wisconsin Madison Gene Expression Center. Library quality was assessed using qubit and Agilent 2100 (High Sensitivity DNa kit) and the libraries were sequence using Novaseq6000 (Novaseq S2 2x50bp).