GSM9960409: MOLM13 FDX1 knockdown (SH3), biological replicate 1; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol reagent and treated with DNase I before library preparation. Poly(A)+ mRNA was enriched from total RNA using oligo(dT) magnetic beads and fragmented. First-strand cDNA was synthesized using random hexamer primers, followed by second-strand cDNA synthesis. Double-stranded cDNA was purified, end-repaired, A-tailed, adapter-ligated, size-selected, PCR-amplified, quality-checked, and sequenced on an Illumina NovaSeq 6000 platform to generate paired-end reads.