GSM9678217: tme7, eacmv, wt, res, 9dpi, Rep3; Manihot esculenta; RNA-Seq
Total RNA was extracted using the Spectrum Plant Total RNA Kit (Millipore-Sigma) and oligo-dT beads mRNA Direct kit, Life Technologies) were used to exclude ribosomal RNA. Poly-A purified mRNA was fragmented and reverse transcribed with SuperScript III RT enzyme (Life Technologies). After double stranded cDNA synthesis, standard Illumina sequencing adapters were ligated to the DNA and unique index tags were added via PCR primers