GSM9948692: BAAM_ABseq; Mus musculus; RNA-Seq
For the mouse study, BALF was collected 14 days after bleomycin administration. BAAM-enriched and BAAM-poor fractions were separated by density-gradient centrifugation and size-based filtration. Cells were subsequently labeled with BD™ AbSeq Oligo Rat Anti-Mouse CD45 and subjected to single-cell RNA sequencing using the BD Rhapsody platform. Beads with oligonucleotide barcodes were added to saturation so that a bead was paired with a cell in a microwell. The cells were lysed in the microwell to hybridize mRNA molecules to barcoded capture oligos on the beads. Beads were collected into a single tube for reverse transcription. Upon cDNA synthesis, each cDNA molecule was tagged on the 5' end with a unique molecular identifier (UMI) and cell barcode indicating its cell of origin. Whole transcriptome libraries were prepared using the BD Rhapsody single-cell whole-transcriptome amplification (WTA) workflow including random priming and extension (RPE), RPE amplification PCR and WTA index PCR.