PacBio sequencing of Candida orthopsilosis: candidemia
High-molecular-weight genomic DNA from Candida orthopsilosis clinical isolates was used for PacBio HiFi sequencing. Libraries were prepared using the SMRTbell Express Template Prep Kit following the manufacturers protocol. DNA was sheared to ~10 kb, end-repaired, A-tailed, and ligated to SMRTbell adapters. Size-selected libraries were sequenced on the PacBio Revio system to generate circular consensus sequencing (CCS) reads for genome assembly and structural variant analysis.