GSM9676249: C2C12, dexamethasone + non conditioned medium, expe 2, rep 2; Mus musculus; RNA-Seq
Total RNA was isolated from C2C12 myotubes by TriPure reagent (Roche, Switzerland). RNA quantity was evaluated using a Nanophen. The quality of the RNA samples were evaluated using a 2100 Bioanalyzer System (Agilent Technologies, USA). All RIN values were above 7, supporting RNA integrity. RNA libraries were prepared for sequencing using standard Illumina protocols. llumina TruSeq Stranded mRNA libraries were prepared using polyA tail selection.