GSM9946476: hippocampus, db/db control group, rep1; Mus musculus; RNA-Seq
Immediately after euthanasia, heart, kidney, hippocampus, liver, and soleus muscle were rapidly harvested, snap-frozen in liquid nitrogen, and stored at −80°C. Total RNA was extracted from each tissue sample using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's standard protocol. RNA quality and integrity were assessed using a NanoDrop spectrophotometer and an Agilent 2100 Bioanalyzer. Stranded mRNA-seq libraries were constructed using poly(A) mRNA enrichment with oligo(dT) magnetic beads. Library preparation was performed following the standard protocol of the NEB Next Ultra RNA Library Prep Kit for Illumina. The constructed libraries were sequenced on the Illumina NovaSeq 6000 platform in paired-end 150 bp (PE150) mode. Library construction and sequencing services were provided by Qingke Biotechnology Co., Ltd.