GSM9376276: control,3; Homo sapiens; RNA-Seq
Total RNA was isolated from oral biopsy samples using the RNeasy Mini Kit (QIAGEN, Hilden, Germany). RNA integrity was verified with an Agilent 2100 Bioanalyzer and samples with RIN values >7.0 were processed for sequencing. Libraries were prepared using the Illumina TruSeq RNA Sample Preparation Kit, quantified with a Qubit 3.0 fluorometer (Thermo Fisher Scientific, Waltham, MA, USA), and sequenced on a NextSeq 500 platform (Illumina, San Diego, CA, USA).