GSM9957341: MAECs, AAV9  overexpressing circKif26b, RNA were treated with RNase R, biological replicate 1; Mus musculus; RNA-Seq
Total RNA from  cells was isolated using Takara RNAiso Plus (Takara, Dalian, China) according to the protocol. RNA-seq libraries were prepared from approximately 1 μg of total RNA using the Ribomoval® rRNA Depletion Kit (Human/Mouse/Rat; Geneseed) and the GENESEED® RNA-seq Library Prep Kit for Illumina (Geneseed), according to the manufacturers' instructions. Briefly, ribosomal RNA was depleted from total RNA, and the remaining RNA was fragmented. First-strand cDNA synthesis and directional second-strand cDNA synthesis were subsequently performed. The resulting cDNA was purified, followed by A-tailing and adapter ligation. Finally, the adapter-ligated DNA fragments were amplified by PCR to generate the sequencing libraries.