RNA-Seq of mus musculus
Single-cell RNA sequencing was performed on osteoclast differentiation and ascites-derived cells using the GEXSCOPE Single-Cell RNA-seq platform (Singleron). Libraries were prepared with barcoded beads, reverse transcription, and cDNA amplification, and sequenced on an Illumina NovaSeq 6000 with 150 bp paired-end reads. Raw data were processed with CeleScope for UMI counting and generation of gene expression matrices for downstream analysis.