16S mouse fecal microbiota SCI Control 4339
DNA extraction from stool samples was performed using the QIAamp Fast DNA Stool Mini Kit (Qiagen), with an additional bead beating lysis step. The V4 region of the 16S rRNA gene was amplified by PCR using 515F and 806R primer pairs. The amplicon libraries were sequenced on the Illumina MiSeq platform.