16S rRNA gene sequencing of the gut microbiota in polycystic ovary syndrome (PCOS) rats following gypenoside intervention
This study aims to investigate the effect of gypenosides on the gut microbiota structure in a rat model of polycystic ovary syndrome (PCOS). Fecal samples were collected from three experimental groups: normal control (Control), PCOS model (PCOS-Model), and gypenoside-treated PCOS (PCOS-Gyp), with 4 biological replicates per group (12 samples total). The V3-V4 region of the bacterial 16S rRNA gene was amplified and sequenced on an Illumina platform (paired-end) to compare microbial community differences among groups, assessing PCOS-associated dysbiosis and the modulatory effects of gypenosides.