ONT RNA-seq of chow-fed mouse liver
Liver RNA from a male C57BL/6N mouse fed a standard chow diet for 19 weeks was used to construct a full-length cDNA library with the Oxford Nanopore Technologies cDNA-PCR Sequencing Kit SQK-PCS109. The cDNA was amplified for 14 PCR cycles, and the library was sequenced on the PromethION platform. Base calling was performed using Guppy implemented in MinKNOW version 2.2.