Oxford Nanopore cDNA sequencing of potato leaf sample M021
Total RNA extracted from potato leaf tissue was reverse-transcribed to cDNA. Sequencing libraries were prepared using the Ligation Sequencing gDNA - Native Barcoding Kit 96 V96 (SQK-NBD114.96; Oxford Nanopore Technologies) and sequenced on a PromethION instrument using an R10.4.1 flow cell. Basecalling and demultiplexing were performed with Dorado.