PacBio full-length 16S rRNA amplicon sequencing of rumen metagenome: leucine-supplemented Angus cow sample L13
PacBio CCS sequencing of PCR amplicons spanning the near-full-length bacterial 16S rRNA gene (27F-1492R) from Angus cow rumen fluid; primers 27F (AGRGTTYGATYMTGGCTCAG) and 1492R (RGYTACCTTGTTACGACTT), 27 PCR cycles, annealing at 60 C.