PacBio RS II whole genome sequencing of ETEC strain E66
Genomic DNA from ETEC isolates was extracted using Qiagen Genomic-tip 500/G kit, sheared into ~10 kb and ~2 kb fragments, and sequenced on PacBio RSII platform. Assemblies were generated using HGAP3 and Falcon pipelines, circularized with Circlator v1.1.0, and error-corrected with Quiver. Annotation was performed using Prokka v1.14.6 with ETEC strain E24377A as reference.