RET_Hispidin_1.5_2
Full-length 16S rRNA gene (V1-V9 regions, ~1,500 bp) amplicon sequencing. Microbial genomic DNA was extracted from cecal contents and amplified using custom barcoded primers. Multiplexed SMRTbell libraries were prepared and sequenced on the PacBio Sequel IIe platform in circular consensus sequencing (CCS) mode to generate high-fidelity (HiFi) reads.