16S rRNA gene amplicon sequencing of fecal microbiota from DSS-induced ulcerative colitis C57BL/6 mice intervened with Radix Hedysari polysaccharide HPS-80
Library construction and sequencing: Total DNA was extracted from samples, and primers were designed targeting conserved regions. Sequencing adapters were added to the termini of primers for PCR amplification. The PCR products were purified, quantified and normalized to construct sequencing libraries. The obtained libraries were subjected to qualitycontrol inspection, and qualified libraries were sequenced on the Illumina NovaSeq 6000 platform. Raw image files generated by highthroughput sequencing (e.g., the Illumina NovaSeq platform) were processed via basecalling to yield raw sequencing reads. The output data were stored in FASTQ (abbreviated as fq) format, which contains sequence information of reads and their corresponding sequencing quality information.