Full-length 16S rRNA (V1-V9) amplicon sequencing of fecal sample from F2 male offspring (control group), replicate 4
Full-length 16S rRNA gene (V1-V9 region) was amplified by PCR with barcoded primers 27F (5'-AGRGTTYGATYMTGGCTCAG-3') and 1492R (5'-RGYTACCTTGTTACGACTT-3'). SMRTbell libraries were constructed by blunt-ligation (Pacific Biosciences) and sequenced on a PacBio Sequel II system (Sequencing Kit 2.0). Circular consensus sequence (CCS) reads were generated with SMRT Link v9.0 (minimum 3 passes, minimum predicted accuracy 0.99).