RNA-Seq of Citrus Bravo under stress conditions
This 2x2 full factorial experimental design evaluates the physiological or transcriptomic response of Citrus bravo plants to heat and water availability across four distinct conditions, using three biological replicates per treatment (n=3). The study includes a baseline control group kept at 28C under well-watered conditions (BC4BC6), a single water-deficit stress group maintained at 28C (BE1BE3), a single heat-stress control group exposed to elevated temperatures of 38C with adequate watering (BC10BC12), and a combined-stress experimental group subjected simultaneously to 38C temperatures and water deficit (BE7BE9).