RNASeq of Trichoderma reesei: Avicel culture
Total RNA was extracted from Trichoderma reesei TU-6 wild-type strain and TrNf1 knockout mutant using the TRIzol method and treated with RNase-free DNase I. RNA quality and integrity were assessed using agarose gel electrophoresis, NanoDrop spectrophotometer and Agilent 2100 Bioanalyzer. For each sample, 1.5 g of total RNA was used for library preparation. RNA sequencing libraries were constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina following the manufacturer's instructions. mRNA was enriched using poly-T oligo-attached magnetic beads, followed by fragmentation, first- and second-strand cDNA synthesis, adaptor ligation, PCR amplification, and library purification. The libraries were sequenced on an Illumina NovaSeq 6000 platform to generate paired-end 150 bp reads.