ddRAD of Pogonus chalceus: adult
A RADtag library was constructed with 15 ml DNA extract (20 ng/ml) using the REs EcoRI and MspI. After digestion, a ligation step added both adapters and an in-line barcode.  For amplification, Q5 Hot Start Taq polymerase was used, and purification was done with magnetic beads before and after PCR.