16S rRNA gene amplicon sequencing of mouse fecal microbiota: Model replicate 5
Fecal microbial DNA was extracted, and the V3-V4 region of the bacterial 16S rRNA gene was amplified using the 343F/798R primer pair. PCR amplicons were used for library construction and paired-end sequencing on the Illumina NovaSeq 6000 platform. Raw sequencing reads were processed using QIIME2 version 2020.11, and amplicon sequence variants were generated using DADA2 and taxonomically annotated against the SILVA database version 138.