Killifish_micro-C_D0R1
Live cells were followed by two-step cross-linking reactions, then the chromatin was digested into 90% mononucleosomes with micrococcal nuclease (MNase). After that,  the ends of digested nucleosomes were repaired, followed by proximity ligation with T4 DNA ligase. After reverse crosslinking reaction, ligated dinucleosomes were selected by gel and purified for library preparation. The library were amplified using the VAHTS Universal Pro DNA library Prep Kit for Illumina and sequenced with 150 bp paired-end reads.