16S rRNA V4 amplicon sequencing of SF1_soil_01
Soil DNA (Qiagen DNeasy PowerSoil) submitted to Novogene Corp (Sacramento, CA, USA). Libraries prepared with the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (cat# E7805L) per manufacturer protocol, amplifying the 16S rRNA V4 region with primers 515F (GTGCCAGCMGCCGCGGTAA) and 806R (GGACTACHVGGGTWTCTAAT). Sequenced on an Illumina NovaSeq 6000 (NovaSeq 6000 SP 300-cycle reagent kit, #20027465), paired-end 250 bp.