WGS of Malus domestica: contrasting disease phenotype bulks of Hanfu  Honeycrispy apple F1 progeny
Genomic DNA was extracted from young leaves of Hanfu  Honeycrispy F apple hybrids by CTAB method and pooled into ten bulks. For each bulk, 0.2 g DNA was fragmented to 350 bp by Covaris LE220R-plus, followed by end repair, A-tailing, adapter ligation, PCR amplification and AMPure XP purification. Library quality was assessed on Agilent 5400 and quantified by qPCR (1.5 nM). Qualified libraries were sequenced on Illumina NovaSeq with PE151 strategy at Novogene (Beijing, China).