Shotgun metagenomic sequencing of Eboliang Hu sediment sample S1
DNA was extracted from 0.25 g of wet sediment using the DNeasy PowerMax Soil Kit. Approximately 100 ng of DNA was fragmented to ~350 bp using a Covaris M220 Focused-ultrasonicator. Metagenomic libraries were prepared using the VAHTS Universal DNA Library Prep Kit for Illumina V3 and sequenced as 2  150 bp paired-end reads on an Illumina NovaSeq 6000.