Shotgun data of Stenocorse sudamericanus CNIN4998: Whole organism
Genomic DNA was extracted using a non-destructive technique with the EZ-10 Spin Kit minipreps DNA Genomic Column Kit (BIOBasic, Toronto, Ontario, Canada) following the manufacturers protocol. Genomic library was prepares using the NEBNext kit following the manufacturers protocol scaled to a 1:5 ratio. Library preparation was carried out using 5.2 ng of extracted DNA suspended in 2.6 L of ultrapure water. Input DNA was fragmented for 5 minutes to a mean fragment size of 200-500 bp using an enzymatic fragmentation system which also enables end repair, and A-tailing with a single enzyme mix.