Amplicon sequencing of 16mer peptide library panned against E. coli and Salmonella
A two-step PCR using phagemid DNA as template was used to amplify the region of interest, adding on a linker sequence, and then add a barcode and Ion Torrent adaptor sequence. Scripts and barcodes available here for analysis: https://github.com/UoN-ADAS-KGough/NGS_Scripts01. L and R motifs were: AQPAMAAS and RSSSGGGS.