RNA-seq of Plasmodium falciparum 3D7 parasites subjected to 1 x EC50 of cryptosporin for one hour before RNA collection - Flask 1
Pelleted parasites were resuspended in TRIzol reagent and RNA was extracted using Direct-zol RNA Miniprep Plus (Zymo). After RNA integrity confirmation, ribosomal RNA depletion, and RNA fragmentation, library preparation was performed per Illumina TruSeq-stranded-total-RNA-sample preparation protocol. Paired-end sequencing was achieved with Illuminas NovaSeq 6000 sequencing system with Agilent Technologies 2100 Bioanalyzer High Sensitivity DNA Chip for quality control. Raw paired-end reads were quality-filtered by the sequencing provider (LC Sciences).