rhizospheric soil metagenome with stack support 2
The bulk soil samples collected from different plants were preserved in liquid nitrogen. Initially, they were fragmented into 2 mL Eppendorf tubes and stored at -20C. One of these aliquots was used for the extraction of genetic material, following the manufacturer's protocol for the DNeasy PowerSoil Pro Kit (Qiagen). For the rhizosphere samples, 30 mL of buffer solution (0.75% KHPO, 0.95% KHPO, and 1% Triton X-100 in ddHO, filter-sterilized through a 0.2 m filter) was added to the tube containing the root with adhered soil. The samples were then shaken for 30 minutes at 200 rpm. The resulting suspension was centrifuged and concentrated into 2 mL Eppendorf tubes for subsequent DNA extraction.