16sRNA sequencing of Healthy Human Microbiome: CGD Patients
Microbial DNA was extracted from stool samples using the QIAamp DNA Stool Mini Kit (Qiagen) following the manufacturers instructions. The stool microbiome was characterized by sequencing the variable region 4 amplicons of 16S rRNA gene according to the 16S metagenomic sequencing library protocol of Illumina. The library was denatured with NaOH, diluted, mixed with 5% PhiX control and finally loaded on MiSeq for high-throughput sequencing (2 x 300 cycle V3 kit).