Stranded RNA-seq of SF1_root_17
Total RNA extracted from 0.2 g frozen root tissue with the Qiagen RNeasy Plant Mini Kit (cat# 74904); all libraries from RNA with concentration >10 ng/uL and RIN >7 (mean RIN 8.1). 1 ug RNA used for stranded cDNA library construction at Novogene Corp (Tianjin, China) with the NEB Directional RNA Library Prep Kit (cat# E7420L) per manufacturer protocol: poly(A) selection with oligo(dT) beads, random fragmentation, first- and second-strand synthesis, end repair, A-tailing, adapter ligation, size selection and PCR enrichment. Sequenced on an Illumina HiSeq 4000, paired-end 150 bp.