Small RNA-seq of Vitis vinifera: cultivar Refosk infected, biological replicate 3
Small RNAs were extracted from young grapevine leaves using the mirVana miRNA Isolation Kit (Thermo Fisher Scientific) following the manufacturers protocol. sRNA libraries were prepared with the Ion Total RNA-Seq Kit v2 and barcoded with the Ion Xpress RNA-Seq Barcode 116 Kit. The yield and size distribution of amplified cDNA libraries were assessed using the Agilent 2100 Bioanalyzer. Libraries were pooled at equimolar concentrations and prepared for sequencing using the Ion PI Hi-Q OT2 200 Kit and Ion PI Hi-Q Sequencing 200 Kit. Sequencing was performed on the Ion Torrent Proton platform according to manufacturersinstructions.