16S rRNA V4 amplicon sequencing of facial skin microbiota, sample W8166.26
16S rRNA V4 region amplicon sequencing of facial skin microbiota. Genomic DNA was extracted using the CTAB method. The V4 region was amplified by PCR using Phusion High-Fidelity PCR Master Mix, and sequencing libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (Cat. No. E7645B; New England Biolabs). Paired-end sequencing was performed on an Illumina NovaSeq 6000 platform.