RNA-seq of gonadal region; female embryos medaka
Total RNA was extracted from pooled stage 40 female medaka embryonic gonads. Sequencing libraries were prepared with 1 g of RNA using the Illumina Stranded mRNA Prep kit, fragmenting mRNA to 20300 bp. Paired-end sequencing (74 bp) was performed on an Illumina NextSeq 500, yielding ~13.6 million reads per library.