GSM9191645: Mouse skin, Infected, Dextran Negative, replicate 4; Mus musculus; RNA-Seq
Following skin sample dissociation (as described above), dextran-positive and -negative macrophages were FACS-sorted (BD FACS Aria) and collected into 100 µl of LB1 buffer from NucleoSpin RNA XS, Micro kit (Macherey Nagel, #740902.50) RNA libraries for RNA-seq were prepared using Illumina Stranded mRNA Prep Ligation following manufacturer's protocol.