GSM9978261: NPAI, mutant IDH1, C6, rep2; Mus musculus; OTHER
Cells were lysed directly in TRIzol followed by isolation of total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described in Paulsen et al., PNAS (2013), PMID: 23345452, and Paulsen et al., Methods (2014), PMID: 23973811). This protocol used single-index barcoded ligation adapters and universal primers for PCR. Size selection for all libraries was via agarose gel excision.