GSM8607564: MOLM13 cells, ZCCHC10, repeat 3; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol reagent. The quantity, integrity,  and purity of RNA were measured by Agilent 2100 Nano 6000 Assay Kit (Agilent Technologies, Santa Clara, CA, USA）. RNA-seq libraries were prepared using NEBNext® Ultra™ RNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA) according to the manufacturer's instructions. Briefly, mRNA was purified from total RNA using oligo (dT)-attached magnetic beads and fragmented in Fragmentation Buffer.  The generated short fragments of mRNA were used a template to synthesize the first strand cDNA with random hexamer primer and RNase H. Second strand cDNA synthesis was subsequently performed using buffer, dNTPs, DNA polymerase I and RNase H. The double strand cDNA fragments were purified with QiaQuick PCR kits (Qiagen, Hilden, Germany), subjected to end repair and 3′ A-tailing, and ligated to sequencing adaptor. The fragments were purified and followed by PCR enrichment to obtain the final cDNA library.