GSM9485394: WT_Ifnb6h_rep1; Mus musculus; ATAC-seq
Bone marrow cells were differentiated into macrophages in DMEM supplemented with 10% heat-inactivated foetal bovine serum (FBS), 2 mM glutamine, 1× non-essential amino acids solution, 100 U/ml penicillin, 100 μg/ml streptomycin, and 20% (v/v) L929-conditioned medium at 37°C with 5% CO2. BMDMs were collected on day 5 and seeded for experiments overnight. HEK293T cells (ATCC CRL-3216, mycoplasma tested but not authenticated) were cultured in DMEM supplemented with 10% heat-inactivated FBS, 2 mM glutamine, 1× non-essential amino acids solution, 100 U/ml penicillin and 100 μg/ml streptomycin. Libraries were prepared with the ATAC-seq kit from Active Motif (53150). Libraries were pooled and sequenced on NovaSeq 6000 (Illumina) to generate 30 million single-end 50-base pair reads for each sample.