16S rRNA V3-V4 amplicon sequencing of soil sample DT_LKJ40_1
16S rRNA gene V3-V4 region amplified with barcoded primers 338F (5'-ACTCCTACGGGAGGCAGCAG-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3'); libraries prepared with NEXTFLEX Rapid DNA-Seq Kit; paired-end 300 bp sequencing on Illumina NextSeq 2000 (Majorbio Bio-Pharm Technology Co., Ltd.)