GSM8295925: IFNb_WT_BMDM_rep3; Mus musculus; RNA-Seq
Total RNA was isolated from cells and purified using RNeasy mini kits and on-column DNAse digestion per manufacturer (Qiagen) protocol. 0.5ug used for library preparation with a TruSeq RNA Sample Preparation Kit v2 (Illumina). The libraries were multiplexed and then sequenced on a HiSeq4000 (Illumina) to generate 30 million single end 50 bp reads. RNA libraries were prepared for sequencing using standard Illumina protocols