GSM9872155: MANF1; Mus musculus; RNA-Seq
Bone marrow cells were isolated from the femurs and tibias of mice and cultured in complete macrophage differentiation medium supplemented with macrophage colony-stimulating factor. The day of bone marrow cell seeding was designated as day 0. During macrophage differentiation, recombinant human MANF was added to the culture medium at a final concentration of 100 ng/mL on days 1, 3, 5, and 7. An equal volume of PBS was added to the control cultures at the corresponding time points. The culture medium was refreshed according to the same schedule while maintaining the indicated concentration of MANF. Total RNA was extracted from BMDMs using TRIzol reagent. RNA quality was assessed before library preparation. RNA-seq libraries were generated using poly(A)-selected mRNA and sequenced on an Illumina platform to generate paired-end reads.