GSM9756715: 4400-02C_ROI_1; Homo sapiens; RNA-Seq
Regions of interest (ROIs) were selected around CD45⁺CD20⁺ immune aggregates with germinal center-like features were UV-cleaved. Released RNA oligos were collected from FFPE tissue and used for library preparation. RNA libraries: GeoMx Whole Transcriptome Atlas (~18,000 genes) and TCR probes. Indexed per manufacturer's Spatial RNA Library Prep Protocol. Libraries were sequenced on Illumina NovaSeq 6000 (paired-end, 2 × 27 bp, i5/i7 dual indexing).