GSM9840886: ko3; Mus musculus; RNA-Seq
Bone marrow cells were isolated from wild-type and Trem2-deficient mice and differentiated into bone marrow-derived macrophages in the presence of M-CSF. Total RNA was extracted from BMDMs and subjected to bulk RNA sequencing. Differential gene expression analysis was performed to identify transcriptional programs regulated by TREM2 in macrophages. Total RNA was extracted from BMDMs using TRIzol reagent. RNA quality was assessed before library preparation. RNA-seq libraries were generated using poly(A)-selected mRNA and sequenced on an Illumina platform to generate paired-end reads.