RNA seq of homo sapiens
Total RNA extraction was performed using the RNeasy Mini Kit (Qiagen, #74106) following the manufacturers protocol. RNA-Seq libraries were prepared using the Illumina TruSeq Stranded mRNA Library Prep Kit. Library quality was measured using the Qubit 2.0 and Bioanalyzer. Bulk mRNA sequencing was conducted at the Northwestern University NUSeq Core Facility. Each sample was sequenced to a depth of 20-25 million reads using NovaSeq X Plus (50 bp read length).