GSM9978324: UOK120 cells, PRCC::TFE3 KO Rep 2; Homo sapiens; ATAC-seq
ATAC-seq was performed according to published protocols using the Illumina Tagment DNA Enzyme and Buffer Kit (20034210, Illumina) (28846090). 70,000 cells were used for each sample. Following the transposition reaction samples were purified using the Zymo DNA Clean and Concentrator Kit (D4014, Zymo) using the manufacturer's protocol. Eluted DNA was amplified using the 2x NEBNext Q5 Hotstart HiFi PCR Master Mix (M0543L, NEB) for 11 cycles with primers from the published protocol (28846090). Amplified libraries were purified using the Zymo DNA Clean and Concentrator Kit.