GSM9956137: iHLC_CnR-H3K4me1_rep1; Homo sapiens; OTHER
Cells were washed with ice-cold DPBS and dissociated into single cells using TrypLE Express. For each CUT&RUN reaction, 500,000 cells were resuspended fresh in ice-cold PBS, except replicate two of HB, iHLC, and HLC, which used 100,000 cells per reaction. Libraries were prepared using the CUTANA ChIC/CUT&RUN Kit v3 and CUTANA CUT&RUN Library Prep Kit according to the manufacturer's protocol and published CUT&RUN methods. Digitonin was used for permeabilization at 0.01% final concentration, except replicate two of HB, iHLC, and HLC, which used 0.05% final digitonin. Primary antibody was added at 0.5 ug per reaction. Libraries were quantified with Qubit dsDNA HS assay and assessed with the Agilent Bioanalyzer High Sensitivity DNA assay.