Metagenomic sequencing of activated sludge: control SBR day 7
Total genomic DNA was extracted from activated sludge using the E.Z.N.A. Soil DNA Kit (Omega Bio-tek, Norcross, GA, USA). DNA integrity was assessed by 1% agarose gel electrophoresis, and DNA purity and concentration were evaluated spectrophotometrically and fluorometrically. Shotgun metagenomic libraries were sequenced on the DNBSEQ-T7 platform using 150-bp paired-end reads (PE150).