GSM9625523: Neuron, wt, RNAseq, day9, rep1; Mus musculus; RNA-Seq
Cells were collected, poly(A)+ RNA was homogenized in a phenol-containing solution, and the sample was isolated by standard ethanol precipitation. RNA-seq libraries were prepared according to the standard Illumina protocols. Briefly, cDNA was synthesized from RNA, end-repaired, and ligated to sequencing adaptors, then PCR amplified and gel purified. The purified DNA libraries were sequenced following the manufacturer's protocols.