GSM9978967: A549_ZIKV_NS5L162G_1; Homo sapiens; RNA-Seq
Total RNA was extracted from A549 cells using a phenol-chloroform-based extraction method. Following phase separation, the RNA-containing aqueous phase was collected, and the RNA was recovered and dissolved in RNase-free water. Polyadenylated mRNA was enriched from total RNA using oligo(dT) magnetic beads and fragmented. Double-stranded cDNA was synthesized, purified, end-repaired, A-tailed, adapter-ligated, size-selected, and PCR-amplified. Qualified libraries were circularized and sequenced on the DNBSEQ-T7 platform using a paired-end 150-bp strategy.