Bombyx mori: early larval stage
Total RNA was extracted from Bombyx mori individuals at the second and fifth larval instars and subjected to high-throughput RNA sequencing. RNA samples were sequenced using the DNBSEQ-T7 platform with a 150 bp paired-end sequencing approach. The generated reads were quality assessed, host-derived reads were removed by alignment to the B. mori reference genome, and the remaining reads were de novo assembled for viral sequence screening.