GSM9959070: FFPE dorsal skin, Adamts2 Q226*/Q226*, 1 month old, sample 1; Mus musculus; RNA-Seq
Nuclei were isolated from FFPE skin sections of Adamts2+/+ and Adamts2Q226*/Q226* mice (n=4 per genotype) using the 10x Genomics protocol for FFPE Tissue Sections for Chromium Fixed RNA Profiling (CG000632), with minor adaptations for mouse skin. Following isolation, nuclei were pelleted (500 × g, 10 min, 4°C), washed in 1X nuclei buffer containing RNase inhibitor, and resuspended in 1X nuclei buffer. An aliquot was stained with trypan blue and counted on an automated counter to determine the concentration of intact nuclei, and the input was adjusted according to the Chromium Fixed RNA Profiling protocol recommendations. Single‑nucleus gene expression libraries were prepared using the Chromium Next GEM Single Cell Fixed RNA Profiling (Gene Expression Flex) chemistry for mouse, including the Chromium Next GEM Single Cell Fixed RNA Sample Preparation Kit (PN‑1000414), following the manufacturer's user guide (CG000691).