GSM9976632: tdT+ Spinal Cord, Sox2-Gli3a transfected; Mus musculus; RNA-Seq
Spinal tissue samples were minced and dissociated. Cells  were isolated via filter straining and centifugation before digestion in TrypLE. The resulting cell suspension was sorted using a Cytoflex SRT sorter before being filtered and washed. Cells were then loaded into a 10X chromium chip. 10X Library preparation according to 10X Genomics' protocol of Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (Dual Index). Illumina NovaSeq 2000 (138 max bps P2) with 10X Genomics Single cell 3' v3