GSM8565572: KO scRNAseq; Mus musculus; RNA-Seq
Cells were obtained by crushing bones followed by red cell lysis with ammonium chloride. cKIT+ cells were enriched with CD117 beads following manufacture's instructions (Miltenyi). Cells were stained in FACS buffer containing PBS + BSA 0.5%. Fc receptors were blocked by incubating with CD16/32 antibody for 10 minutes on ice followed by staining with Lineage, Sca1 and cKIT fluorochrome-labeled antibodies for 20 minutes on ice. DAPI-Lineage-Sca1+cKit+ cells were sorted in a FACS Aria II. Library was constructed following manufacture's instructions (single cell 5' v2 10X genomics)