GSM8834135: BM_HSC_1_RNA; Mus musculus; RNA-Seq
For mRNA seq, total RNA was extracted from HSC using Trizol (Invitrogen) and 500ng RNA was used for construcation of library. For cut-tag  and ATAC seq, DNA was fragmented by Tn5 and cleaned by DNA extract beads. For RNA seq, libraries were prepared according to the illumina standard instructions. For cut-tag  and ATAC seq, DNA libraries were prepared using the TruePrep Index Kit v2 (Vazyme, TD503). For scRNA seq, libraries were prepared using the Chromium Single Cell 3' Reagent Kit v2. For spatial transcriptomics,  libraries were prepared using the Stereo-seq Transcriptomics Set for Chip-on-a-slide (STOmics).