Cas13 DUF targeting cRACE RNA endpoint sequencing
cRACE RNA sequencing of Escherichia coli K-12 NEB 5-alpha expressing Cas13 with the DUF ancillary RNase under targeting conditions. Total RNA was treated with T4 RNA ligase to ligate RNA 5-prime and 3-prime ends, including intramolecular circularization and intermolecular ligation, preserving native and cleavage-generated RNA ends. Paired-end sequencing was used to identify ligation-spanning reads and map RNA endpoints.