GSM9978536: Pho7-3sAID, Pho7-, rep2; Schizosaccharomyces pombe; RNA-Seq
RNA was purified from cells using the RNeasy Mini Kit by following the manufacturer's instructions. Messenger RNA was isolated from 1 µg of total RNA using Poly(A) mRNA Magnetic Isolation according to the manufacturer's instructions. RNA-seq libraries were constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina with amplification using Q5 Master Mix. cDNA synthesis and conversion to double-strand DNA were performed as part of the library preparation workflow. DNA fragments in the 200–700 bp range were enriched using AMPure XP beads.