GSM9927064: Liver RNA-seq of mouse n12165: western diet plus 0.1% AB-6382 late treatment, LH replicate 7; Mus musculus; RNA-Seq
Total RNA was extracted from liver tissues using the QIAGEN RNeasy Mini Tissue Extraction Kit, with genomic DNA removed via DNase treatment. Libraries were constructed from 100 ng of RNA per sample using the QIAGEN QIAseq UPXome RNA Library Kit with QIAseq FastSelect rRNA depletion and N6-T reverse transcription primers. Sequencing was performed on the MGI DNBSEQ-T7 platform.