GSM9956257: DE_ATAC_rep1; Homo sapiens; ATAC-seq
Cells were dissociated with TrypLE Express, neutralized in culture medium, pelleted, counted, washed with ice-cold PBS, resuspended in ATAC-seq lysis buffer, centrifuged at 4 deg C, and the pellet was used immediately for tagmentation. Libraries were prepared using the ActiveMotif ATAC-seq kit according to the manufacturer's protocol and published ATAC-seq methods. Libraries were quantified with Qubit dsDNA HS assay and assessed with the Agilent Bioanalyzer High Sensitivity DNA assay.