GSM9287390: D100 THRB2–/NRL– cells, biol rep 3; Homo sapiens; RNA-Seq
Total RNA was isolated from each sample using the RNAeasy mini spin kit (Qiagen 74104) according to the manufacturer's instructions. Libraries for sequencing were prepared using the TruSeq Stranded Total RNA Library Prep kit (illumina 20020599) for humans. Libraries were sequenced on a NovaSeq6000 and 30 million 2 x 150 nucleotide reads were collected for each sample.