GSM9814283: CD8+ TVM T cells, midaged, male, unstimulated, biological replicate 2; Mus musculus; RNA-Seq
Up to 100,000 sorted cells were preserved in 50 µL Zymo DNA/RNA Shield. Total RNA was subsequently extracted using a bead-based extraction approach. Total RNA was extracted from sorted CD8 T cell populations preserved in Zymo DNA/RNA Shield. Sequencing libraries were generated using a 3′ end counting RNA-seq approach. mRNA was reverse transcribed using a poly(dT)VN primer, followed by second-strand synthesis, tagmentation, indexing, and amplification. Unique molecular identifiers (UMIs) and unique dual indices (UDIs) were incorporated to enable PCR deduplication and minimize index hopping.