GSM9355580: K14CreER_Cas9_gPtger4_Rep2; Mus musculus; RNA-Seq
10,000 EpdSCs per condition were FACS-sorted directly into room temperature buffer RLT (QIAGEN) and immediately flash frozen. Two biological replicates were used per condition, with 2 mice pooled per biological replicate. Total RNA was isolated with RNeasy Mini Kit (QIAGEN) as per manufacturer's instructions. Libraries were then prepared using Illumina TruSeq low-input mRNA library kit and sequenced on Illumina NextSeq using 50-bp paired-end reads (100 million reads). PCR amplified library from genomic DNA