lung
The viable lung cells isolated from three mice in each group (control and LysM-Spz1 KO) were dissociated according to the procedure described above and pooled for subsequent single-cell RNA sequencing analysis. To achieve a target recovery of approximately 15,000 single cells, 20,000 viable cells from each group were loaded onto the 10x Genomics Chromium Single Cell platform. Single-cell libraries were prepared using the Chromium Single Cell 3 GEM, Library & Gel Bead Kit v3 (10x Genomics) according to the manufacturers instructions. The resulting libraries were sequenced on an Illumina NovaSeq 6000 platform with a sequencing depth of at least 100,000 reads per cell using 150-bp paired-end (PE150) sequencing.