Capillary Sanger sequencing of the 3-prime terminal amplicon of infectious bronchitis virus isolate IBS130/2015
The 3-prime genome terminus of infectious bronchitis virus isolate IBS130/2015 was generated using an oligo(dT)-based reverse-transcription approach followed by PCR amplification. The purified terminal amplicon was sequenced independently in the forward and reverse directions by capillary Sanger sequencing to determine and confirm the 3-prime genome terminus.