GSM9481801: Irf1_ChIP_untreated_rep2; Mus musculus; ChIP-Seq
Bone marrow cells were differentiated into macrophages in DMEM supplemented with 10% heat-inactivated foetal bovine serum (FBS), 2 mM glutamine, 1× non-essential amino acids solution, 100 U/ml penicillin, 100 μg/ml streptomycin, and 20% (v/v) L929-conditioned medium at 37°C with 5% CO2. BMDMs were collected on day 5 and seeded for experiments overnight. HEK293T cells (ATCC CRL-3216, mycoplasma tested but not authenticated) were cultured in DMEM supplemented with 10% heat-inactivated FBS, 2 mM glutamine, 1× non-essential amino acids solution, 100 U/ml penicillin and 100 μg/ml streptomycin. Illumina sequencing libraries were prepared from the ChIP and Input DNAs by the standard consecutive enzymatic steps of end-polishing, dA-addition, and adaptor ligation. Steps were performed on an automated system (Apollo 342, Wafergen Biosystems/Takara). After a final PCR amplification step, the resulting DNA libraries were quantified and sequenced on Illumina's NextSeq 500 (75 nt reads, single end).