GSM7750135: BMSCs,blank,Day7,2; Rattus norvegicus; RNA-Seq
Total RNA was extracted using the TRlzol reagent (lnvitrogen, CA, USA) according to themanufacturer's protocol. RNA purity and quantification were evaluated using the NanoDrop 2000spectrophotometer (Thermo Scientific, USA). RNA integrity was assessed using the Agilent 2100Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The libraries were constructed using VAHTS Universal V6 RNA-seq Library Prep Kit according to the manufacturer' s instructions. Thetranscriptome sequencing and analysis were conducted by OE Biotech Co., Ltd. (Shanghai, China).