Shotgun metagenomic sequencing of air metagenome
Total genomic DNA was extracted from PM2.5 filter samples using [your kit]. The DNA was fragmented to ~300 bp using a Covaris M220 instrument. Paired-end libraries were constructed using the TruSeq DNA Sample Prep Kit (Illumina), involving Y-shaped adapter ligation, magnetic bead-based size selection, and PCR enrichment. Libraries were denatured with NaOH to generate single-stranded fragments. Cluster generation was performed via bridge PCR on the flow cell, followed by linearization. The libraries were sequenced on an Illumina HiSeq 4000 platform using HiSeq 3000/4000 SBS Kits with 150 bp paired-end reads.