GSM9848695: TCRs Hodgkin lymphoma treatment timepoint 4; Homo sapiens; RNA-Seq
PBMCs collected in EDTA-coated tubes were purified using Ficoll gradient centrifugation and viably cryopreserved at -196°C. PBMC samples were thawed at 37°C. Dead cells were removed by gradient centrifugation using Ficoll-Paque PLUS (GE Healthcare) or by magnetic column-based depletion (Miltenyi MACS Dead Cell Removal Kit and LS columns). Droplet-based scRNA-seq on six PBMC samples was performed using the Chromium (10x genomics, Pleasanton, CA) 5' library protocol with simultaneous single cell V(D)J enrichment for human B and T cells according to the manufacturer's protocol.