GSM9956247: H9ESC-derived-DE-to-HB_DE+2d_RNA; Homo sapiens; RNA-Seq
Stem cells and differentiated cells were grown in 6-well plates, collected into Trizol, and stored at -80 deg C. Total RNA was isolated from the aqueous phase after chloroform extraction, isopropanol precipitation, ethanol washing, and resuspension in nuclease-free water. For the DE-to-HB time course, RNA was additionally cleaned with on-column DNase digestion using the Qiagen RNeasy kit, and RIN > 7 was confirmed. For the five-stage differentiation series, RNA was submitted to BGI for mRNA enrichment, fractionation, cDNA generation, and 100 nt paired-end strand-specific mRNA sequencing by DNBSEQ. For the DE-to-HB time course, rRNA was removed with NEBNext rRNA Depletion Kit v2 and libraries were generated with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina.