Capillary Sanger sequencing of the 5-prime RACE amplicon of infectious bronchitis virus isolate IBS130/2015
The 5-prime genome terminus of infectious bronchitis virus isolate IBS130/2015 was amplified from viral cDNA using 5-prime rapid amplification of cDNA ends. The resulting terminal amplicon was cloned into a pJET cloning vector, and the recombinant clone was sequenced by capillary Sanger sequencing to determine the 5-prime genome terminus.