GSM9826729: rep2_Cancer_Neuron-cancer; Mus musculus; RNA-Seq
Total RNA was isolated from Mus musculus in vitro culture samples representing cancer cells, neurons, T cells, and their co-culture or triple co-culture conditions, with three biological replicates per condition. RNA quality and integrity were assessed using the Agilent BioAnalyzer Eukaryote Total RNA Pico assay prior to RNA-seq library preparation. RNA-seq libraries were prepared from total RNA isolated from Mus musculus E0771-Ova-GFP cancer cell, DRG, T cell, co-culture, and triple co-culture samples. Stranded mRNA libraries were generated using the KAPA RNA HyperPrep kit with poly(A) selection. Libraries were sequenced on an Illumina NextSeq platform using single reads.