GSM9191133: K26; Homo sapiens; RNA-Seq
High-quality total RNA was isolated from approximately 2 x 10^4 T cell supernatant-treated keratinocytes or fibroblasts using TRIzolTM Reagent (InvitrogenTM; 15596026). cDNA was prepared using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara). Library construction was performed using the NexteraXT DNA sample preparation kit (Illumina) using half the recommended volumes and reagents. Libraries were pooled and quantified using a Qubit Fluorometer (Life Technologies).  Dual-index, single-read sequencing of the pooled libraries was carried out on a HiSeq 2500 sequencer (Illumina) with 58-base reads, using HiSeq v4 Cluster and SBS Kits (Illumina) with a target depth of 5 million reads per sample.