GSM9168699: hPCO_THA_133; Homo sapiens; RNA-Seq
For bulk RNA sequencing, total RNA was extracted from day 20 hCPOs (2-3 biological replicates per group) using the FastPure Cell/Tissue Total RNA Isolation Kit (Vazyme, RC101) following the manufacturer's protocol. Prior to extraction, hCPOs were mechanically dissociated using scalpel and fine forceps. The libraries were constructed using Truseq RNA Sample Prep Kit v2 and were sequenced on the Illumina NovaSeq X Plus platform.