GSM9864047: MG1655 ΔaraD-B pth-Tn::Cm, replicate 2; Escherichia coli; RNA-Seq
Total RNA was extracted from bacterial pellets using the phenol-chloroform extraction method. After quality verification of the extracted total RNA, ribosomal RNA (rRNA) was depleted to enrich mRNA. The enriched mRNA was randomly fragmented into short fragments using Fragmentation Buffer, and strand-specific RNA libraries were constructed as previously described (Parkhomchuk et al., 2009, Transcriptome analysis by strand-specific sequencing of complementary DNA).