GSM9737291: Ctrl-1; Homo sapiens; RNA-Seq
RNA was harvested using TRIzol Regant mRNA was enriched from total RNA using Oligo dT magnetic beads. After fragmentation, the first-strand cDNA was synthesized with random hexamer primers. Subsequently, the second-strand cDNA was synthesized using dUTP instead of dTTP. Following end repair, A-tailing, adapter ligation, fragment selection, amplification and purification, the strand-specific library was successfully constructed.