GSM9956068: ESC_ChIP-Input_rep1; Homo sapiens; ChIP-Seq
For each stage, 2-15 x 10^6 cells were crosslinked with 1% formaldehyde. Cell pellets were lysed, nuclei/chromatin were resuspended in sonication buffer, and chromatin was sonicated to approximately 200-500 bp fragments using a BioRaptor Pico. Fragmented chromatin was diluted in immunoprecipitation buffer and incubated overnight at 4 deg C with antibody. The library-preparation methods list CTCF, RAD21, and Rabbit IgG antibodies. Dynabeads Protein A were added, beads were washed, immunoprecipitated DNA was eluted, and 5-10 ng DNA was used for library preparation.