GSM9978348: UOK120 cells, PRCC∆PRW::TFE3 Rescue Rep 3; Homo sapiens; RNA-Seq
Total RNA extraction was performed using RNeasy Mini Kit (74104, Qiagen) and RNase-Free DNase Set (79254, Qiagen) according to the manufacturer's instructions. 1,000,000 cells were used for each sample. Cells pellets were collected and stored at -80˚C until RNA extraction. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.