GSM9965342: WT_FEMALE_REP3; Mus musculus; RNA-Seq
RNA was extracted from extensor digitorum longus muscle using an Invitrogen PureLink RNA Mini Kit (12183205, ThermoFisher Scientific). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. Following fragmentation, first-strand cDNA was synthesized using random hexamer primers, followed by second-strand cDNA synthesis. Libraries were finalized via end repair, A-tailing, adapter ligation, size selection, and PCR amplification, then sequenced on the Illumina NovaSeq X Plus platform (paired-end 150 bp format)