Cas13 EV nontargeting cRACE RNA endpoint sequencing
cRACE RNA sequencing of Escherichia coli K-12 NEB 5-alpha expressing Cas13 with an empty vector under nontargeting conditions. Total RNA was treated with T4 RNA ligase to ligate RNA 5-prime and 3-prime ends, including intramolecular circularization and intermolecular ligation, preserving native and cleavage-generated RNA ends. Paired-end sequencing was used to identify ligation-spanning reads and map RNA endpoints.