GSM9651126: scRNA-seq, hPSC_IL7high, GEX; Homo sapiens; RNA-Seq
IL-7 high cells were depleted of MS-5 cells using msCD29-PE antibody (Biolegend) and MojoSort™ Mouse anti-PE Nanobeads (Biolegend).  This was followed by B cell enrichment using EasySep™ Human B Cell Enrichment Kit II Without CD43 Depletion (Stem Cell Technologies). IL-7 low cells were enriched via CD19+ selection (Stem Cell Technologies). GEX, BCR and ADT Libraries were prepared according to Chromium Next GEM Single Cell 5' Kit v2 kit with Feature Barcoding technology for Cell Surface Protein User Guide. Gene expression, BCT and ADT libraries were sequenced together on a NovaSeq 6000 platform (Illumina, San Diego, CA)