GSM9168686: hPCO_GFP_1; Homo sapiens; RNA-Seq
For bulk RNA sequencing, total RNA was extracted from day 20 hPCOs (2-3 biological replicates per group) using the FastPure Cell/Tissue Total RNA Isolation Kit (Vazyme, RC101) following the manufacturer's protocol. Prior to extraction, hPCOs were mechanically dissociated using scalpel and fine forceps. The libraries were constructed using SMART-Seq_V4 Ultra Low Input RNA Kit and were sequenced on NovaSeq X Plus platform for FACs samples.