GSM8480339: testis cell; Sus scrofa; ncRNA-Seq
Small RNA sequencing was completed by the Shanghai Institute of Biochemistry and Cell Biology, with procedures and analyses consistent with previous reports[33]. Briefly, 10 µg of total RNA was utilized to construct a small RNA cDNA library. Initially, adaptor ligation was performed, followed by first-strand cDNA synthesis and PCR amplification. Finally, the purified DNA fragments were obtained for sequencing using the Illumina HiSeq 2000 platform. The analysis of small RNA sequencing results was conducted using Bowtie software, with the reference genome for pigs being Sus scrofa version Sscrofa10.2. Additionally, the tRNA database (available at http://gtrnadb.ucse.edu/) was used for analyzing the source information of tsRNA sequences.