GSM9967072: Diabetes mellitus and periodontitis mouse brain, pooled RNA; Mus musculus; miRNA-Seq
Brain samples from six mice per experimental group were pooled prior to library preparation. Total RNA, including miRNAs, was extracted from brain samples using the mirVana miRNA isolation kit (Thermo Fisher Scientific, Waltham, MA, USA). Extracted total RNA was quantified using a NanoDrop One spectrophotometer (Thermo Fisher Scientific). RNA quality was evaluated using an Agilent 2100 Bioanalyzer with the Agilent RNA 6000 Nano kit (Agilent Technologies, Santa Clara, CA, USA). Sequencing libraries were constructed using the QIAseq miRNA Library Kit (Qiagen, Hilden, Germany) and QIAseq miRNA NGS 96 Index IL (Qiagen) according to the manufacturer's protocols. Library quality was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies, Inc.) and the Agilent High Sensitivity DNA Kit (Agilent Technologies, Inc.). Pooled libraries were sequenced using Illumina NextSeq 500 (Illumina, Inc., San Diego, CA, USA) with 75-base-pair (bp) single-end reads.