WT_N2_F30_Line5
For this experiment, a single mother was isolated from each of the strains. These isolated mothers were used to propagate populations, which were synchronized by bleaching. This synchronized population served as the P0 founder population for the experiment. The populations were collected and stored at -80C. From these initial populations, five mothers were isolated and placed on separate plates, serving as the founding mothers of the experiment (N=5). Throughout the experiment, one L4 stage larva from each strain was transferred individually to a new plate in every generation, spanning 30 generations. Upon reaching the F30 generation, a population was propagated and synchronized via bleaching to collection for whole genome sequencing (n=3000). All the synchronized populations were collected at the young adult stage. Library_selection: AMPure PB beads for size-selection of SMRTbell libraries (Depletion of molecules below 5 kb) were diluted with PacBio Elution Buffer to 35% (volume by volume) and then used for size-selection of the libraries.