GSM9914108: CAST, Liver, baseline_day0, rep2; Mus musculus; RNA-Seq
Non-parenchymal hepatic mononuclear cells (HMNCs) were isolated from Mr1+/+ and Mr1-/- B6-MAIT^CAST mice receiving CCl₄ 1, 12 or 26 days earlier. Corn oil-treated mice were used as controls. Three mice were included per experimental group as biological replicates. Freshly isolated HMNCs were immediately processed for RNA extraction using the PureLink RNA Mini Kit (Thermo Fisher Scientific) following the manufacturer's instructions. RNA samples were stored at -80°C until library preparation and sequencing. RNA quality and integrity were assessed using an Agilent 2100 Bioanalyzer, and all samples exhibited a 28S/18S rRNA ratio of ∼2.0. Library preparation and sequencing were performed at the London Regional Genomics Centre (London, Canada) using an Illumina NextSeq High Output 75-cycle kit on an Illumina NextSeq platform, following the manufacturer's mRNA-Seq workflow. Sequencing was performed to a depth of approximately 30 million single-end reads per sample.