scRNAseq of mouse lymph nodes
Single-cell RNA sequencing was performed on viable cells isolated from the right inguinal lymph nodes of female C57BL/6 mice 24 hours after the second intramuscular vaccination. Five mice were included per treatment group. Live 7-AAD-negative cells were purified by fluorescence-activated cell sorting and processed using the 10x Genomics Chromium Next GEM Single Cell 5 workflow. Libraries were sequenced using paired-end Illumina sequencing.