Genome skimming of Pileolaria sp.: specimen MBM288585
Genomic DNA was extracted from ethanol-preserved tissue using the DNeasy Blood and Tissue Kit (Qiagen). Whole-genome sequencing libraries were constructed with 0.2 g of input DNA, which was fragmented to an average size of 350 bp using a Covaris LE220R-plus sonicator. Fragments were end-repaired, A-tailed, and ligated with Illumina adapters followed by PCR amplification. The final libraries were quality-controlled on an Agilent 5400 system and quantified by real-time PCR. Pooled libraries were sequenced on an Illumina NovaSeq X Plus platform using a paired-end 150 bp (PE150) strategy to generate approximately 10 Gb of raw data per sample. This genome-skimming approach aims to obtain high coverage of organelle genomes and ribosomal DNA for phylogenetic analysis.