Illumina NovaSeq X paired end sequencing of SAMD01797547
Total RNA was extracted from mouse bone marrow-derived macrophages using the FastGene RNA Premium Kit. Poly(A)+ RNA was enriched from total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module. Sequencing libraries were prepared from the enriched mRNA using the NEBNext Ultra II Directional RNA Library Prep Kit according to the manufacturer's instructions. Libraries were constructed for paired-end sequencing.