GSM9932447: peripheral nasal retina; post conception day 82; Homo sapiens; RNA-Seq
After the removal of cornea and lens, retina was detached from RPE/choroid and separated into temporal and nasal sections. These were further dissected to retrieve central pieces around the presumptive fovea and peripheral parts distal to the optic nerve head on the nasal side. For single-cell long-read sequencing we dissected temporal-central and nasal-peripheral retina of a gestational age 82 eye. Retinas were digested in Papain (Worthington) for 10 min at 37 C, followed by mechanical dissociation using a P1000 pipette. Ovomucin (Worthington) was added to the solution to stop the reaction. The suspension was passed through a 70 µm cell strainer and spun down for 7 min at 400 g, 4° C.