RNAseq of human heart tissue from healthy and failing hearts
RNAseq of human heart tissue from healthy and failing hearts
The hearts were collected and snap-frozen in liquid nitrogen by BioIVT under proper licence and consent agreement; then imported under proper MTA and stored in -80 degree freezer with HTA licence before usage. Total RNA from the heart tissue was extracted using TRIzol and treated with DNAse (ThermoFisher Scientific, AM1906) to eliminate genomic DNA contamination. The quality and integrity of total RNA samples were checked using a 2100 Bioanalyzer (Agilent Technologies).  Samples were enriched for 3'-polyadenylated transcripts. Library preparation of samples was performed using the Illumina TruSeq Stranded mRNA Sample Preparation Kits (Illumina).