GSM9970596: DOX-treated H9C2 cells 2; Rattus norvegicus; RNA-Seq
Total RNA was extracted from H9c2 cells using TRIzol reagent according to the manufacturer's instructions. The extracted RNA was assessed for concentration, purity, and integrity prior to library preparation. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, first-strand cDNA was synthesized using random hexamer primers, followed by second-strand cDNA synthesis. The sequencing library was prepared through end repair, A-tailing, adapter ligation, size selection, amplification, and purification. Library quality was assessed by Qubit and real-time PCR for quantification and by Bioanalyzer for size distribution. The libraries were sequenced on an Illumina NovaSeq 6000 platform using paired-end sequencing.