GSM8441658: iPSC_Hepatocytes_233; Homo sapiens; ATAC-seq
A total of 50,000 to 100,000 cells were centrifuged at 550g for 5min at 4°C. The cell pellet was washed with cold PBS and resuspended in 50μL cold lysis buffer (10mM Tris-HCl, pH7.4, 10mM  NaCl, 3mM MgCl2, 0.1% NP-40/IGEPAL CA-630) and immediately centrifuged at 550g for 10min at 4°C. Nuclei were resuspended in the Nextera transposition reaction mix (25μL 2x TD Buffer, 2.5μL Nextera Tn5 transposase (Illumina Cat #FC-121-1030), and 22.5μL nuclease free H2O) on ice, then incubated for 45min at 37°C. The tagmented DNA was then purified using the Qiagen MinElute kit eluted with 10.5μL Elution Buffer (EB). Ten microliters of purified tagmented DNA was PCR amplified using Nextera primers for 12cycles to generate each library. PCR reaction was subsequently cleaned up using 1.5x AMPureXP beads (Agencourt) and concentrations were measured by Qubit. Libraries were paired-end sequenced on the Illumina Libraries were paired-end sequenced on the Illumina Novaseq 6000 platform (100bp read length).