Metagenomic sequencing of chigger from Thailand
Illumina library preparation and sequencing of the samples were performed in the Centre for Genomic Research (CGR) at the University of Liverpool. NEBNext Ultra II FS kit paired-end libraries (2x150 bp) were generated and sequenced with NovaSeq 6000 using S4 chemistry. Raw fastq files were trimmed for the presence of Illumina adapter sequences using Cutadapt v1.2.1 with option -O 3; the reads were further trimmed using Sickle v1.200 with a minimum window quality score of 20; reads shorter than 15 bp after trimming were removed.