GSM9092584: Naive_CD4_T_cells_2_H3K27ac CUT&Tag; Homo sapiens; OTHER
After tamoxifen treatment, the 1 × 105 Treg cells (CD4+ CD25+ GFP+) from the TME of Nat10+/+ Foxp3GFP-cre-ERT2 or Nat10fl/fl Foxp3GFP-cre-ERT2 mice were flow cytometry-sorted and prepared for CUT&Tag, with each condition replicated twice. The procedure followed a standard protocol, wherein a single-cell suspension was obtained for each condition, and 100,000 cells were utilized for each experimental replicate. Cell enrichment was achieved by sorting them with ConA-magnetic beads. The DNA fragments were supplemented with 1 μL spike-in (Vazyme), extracted using DNA extract beads, and then amplified by PCR for 12 cycles with the TruePrep index kit for Illumina