WGS of Galaxias maculatus: adult muscle tissue
samples were cross-linked under vacuum infiltration for 30 min with 3% formaldehyde at 4 and quenched with 0.375M final concentration glycine for 5 min. The cross-linked samples were subsequently lysed. Endogenous nuclease was inactivated with 0.3% SDS, then chromatin DNA were digested by 100U MboI (NEB), and marked with biotin-14-dCTP (Invitrogen) and then ligated by 50U T4 DNA ligase (NEB). After reversing cross-links, the ligated DNA was extracted through QIAamp DNA Mini Kit (Qiagen) according to manufacturers instructions. Purified DNA was sheared to 300- to 500-bp fragments and were further blunt-end repaired, A-tailed and adaptor added, followed by purification through biotin-streptavidinmediated pull-down and PCR amplification.