RNA-Seq of Lotus corniculatus: B2 root, 2.0 mM Al, replicate 2
Total RNA was extracted from root tissue of L. corniculatus cv. Qiangui No.1. mRNA was enriched by oligo(dT) beads (PolyA selection), fragmented, and reverse-transcribed to synthesize cDNA. Sequencing libraries were constructed by end repair, adapter ligation and PCR amplification, and sequenced as paired-end reads on the Illumina NovaSeq 6000 platform.