Deep-seq of adapted spacer library
The DNA from the second cycle of the adapted library was amplified using 2x Phanta Flash master mix. 50 microlitres of the amplified PCR reaction was loaded on a 2% (w/v) agarose gel and electrophoresed for 35 minutes at 120V. Following gel separation, the expanded band of approximately 240 bps was excised from the gel and purified using the FavorPrep Gel/PCR Purification Mini Kit. The PCR product described above was used for the preparation of the library and was sequenced using (150PE) MGI- the DNBSEQ-G400 machines according to the manufacturers instructions.