GSM8038013: Ctrl1 H3K27ac CUT&Tag; Mus musculus; OTHER
Treg cells (CD4+ CD25+ YFP+) from the TME of Nat10+/+ Foxp3cre-ERT2 or Nat10fl/fl Foxp3cre-ERT2 mice were sorted by flow cytometry and prepared for CUT&Tag ChIP-seq and other muti omics sequencing, each in two biological replicates. CUT&Tag ChIP-seq was performed according to a standard protocol. The DNA fragments added 1 μL spike in (Vazyme) and were extracted by DNA extract beads, then amplified by PCR for 12 cycles with TruePrep index kit for illumine. Each individual library has been paired-end sequenced on an Illumina NovaSeq platform.