Mitochondrial membrane lipid cardiolipin controls fiber-type adaptations in ageing muscle via ERRγ
Mitochondrial membrane lipid cardiolipin controls fiber-type adaptations in ageing muscle via ERRγ
The termination was performed by decapitation for tissue collection. Dissections of EDL and Soleus muscles was performed for RNA isolation and subsequent sequencing. Total RNA was extracted from snap-frozen tissues using TRI Reagent (Sigma-Aldrich) and purified by isopropanol precipitation according to the manufacturer's instructions. Libraries were prepared using the Universal Plus mRNA-seq with NuQuant protocol (Tecan) as recommended by the manufacturer. Libraries were quantified with NuQuant using the CLARIOstar Plate Reader (BMG Labtech) and quality checked using a TapeStation instrument (Agilent Technologies)