GSM9497160: Donor 213 Stage 4 NK cells from 15-C-DMSO culture; Homo sapiens; RNA-Seq
Total RNA was extracted from sorted NK cells using the Quick-RNA Microprep Kit (Zymo Research). RNA integrity (RIN) was assessed to be >9.5 using an Agilent Bioanalyzer. cDNA libraries were generated using the SMART-Seq Total RNA Pico Input Kit with ZapR and UMIs (Takara) according to the manufacturer's recommendations. This kit utilizes ZapR for ribosomal RNA depletion.