GSM9632814: Mouse lung, infected + doxycycline, biological replicate 3; Mus musculus; RNA-Seq
Organs were snap-frozen in liquid nitrogen and homogenized in TRIzol reagent (Thermo Fisher). Total RNA was isolated according to the manufacturer's protocol. RNA integrity was assessed using an Agilent Bioanalyzer (RIN >= 7 required). Strand-specific RNA-seq libraries were prepared from 1 ug total RNA after ribosomal RNA depletion (RiboZero) using the KAPA RNA HyperPrep Kit. Libraries were size-selected (200-500 bp) and sequenced as 150 bp paired-end reads on an Illumina NovaSeq 6000 platform.