GSM9982645: AntPBX ChIP-nexus (anti-FLAG) on S2 cells co-expr AntHOX1a::3xHA, AntMeisHM::3xMyc and AntPBX::3xFLAG, rep 2; Drosophila melanogaster; ChIP-Seq
ChIP-nexus was performed in Drosophila S2 cells co-expressing AntHOX6a::3xHA, AntHOX1a::3xHA, AntMeisHM::3xMyc and AntPBX::3xFLAG (two biological replicates) using anti-HA and anti-FLAG antibodies (Cell Signaling Technology) at manufacturer-recommended concentrations. Libraries were prepared following the standard ChIP-nexus protocol adapted to cell cultures (https://labs.stowers.org/zeitlinger/resources) and sequenced on a Singular Genomics G4 platform.