ITS sequences of cotton root
Genomic DNA was amplified via PCR with  ITS2 (ITS2-F: GTGAATCATCGARTCTTTG, ITS2-R: TCCTCCGCTTATTGATATGC) primers. Final library quantity was assessed by Qubit 2.0 (ThermoFisher, Massachusetts, USA) and quality was assessed by TapeStation D1000 ScreenTape (Agilent Technologies Inc., California, USA). Illumina 8-nt dual-indices were used. Equimolar pooling of libraries was performed based on QC values and sequenced on an Illumina Miseq with a read length configuration of 250PE for 0.1M PE reads per sample (50K in each direction).