Human placenta bulk RNA-seq, 68 weeks
Placental villous tissue samples were collected from early first trimester (68 weeks gestational age) elective terminations. Total RNA was extracted from each sample and used to prepare poly-A selected, paired-end libraries for transcriptomic profiling. The study design aimed to capture early transcriptional signatures associated with fetal sex, with five male and five female conceptuses processed and sequenced independently.