RNA-Seq of homo sapiens: NK cells
Total RNA was extracted from the frozen samples with TRIzol (Invitrogen, Carlsbad, USA). The following conversion of total RNA into a cDNA was performed using mRNA-seq Lib Prep Module for Illumina (ABclonal, China) according to the manufacturer's instructions. Adaptor ligation and the addition of indexes were conducted using RNA Adapter Module 96 Index for Illumina (ABclonal, China). The final average fragment size was 300 bases.