Intramuscular fat cells
RNA-seq library construction, sequencing and analysisRNA-seq library construction and sequencing were performed by LC-Bio Technologies Co., Ltd. (Hangzhou, Zhejiang, China). Raw sequencing data were processed and trimmed using fastp v0.23.4_py36 with parameters --detect_adapter_for_pe -u 35 -w 4 -h. The clean reads were aligned to the Sus scrofa reference genome (Scrofa11.1) using STAR v2.7.11a. Gene expression quantification was performed with subread v2.0.6 via featureCounts using parameters -p -t exon -g gene_id. Differentially expressed genes (DEGs) were identified using the DESeq2 v1.42.1 package. Public RNA-seq datasets (GSE201951, GSE99670) were reanalyzed following the same workflow. For DEG functional enrichment analysis, the KEGGREST v1.38.0 and clusterProfiler v4.6.2 packages were utilized.