Amphimedon queenslandica, cloud-spot embryos, polyA-rRNA-depleted_3
Total RNA was extracted using TRIzol (Sigma-Aldrich) and DNA was removed with Deoxyribonuclease I (Invitrogen). To increase the proportional representation of low abundance bacterial symbionts, bacterial RNA was enriched by removing polyA using the Poly(A) Purist MAG kit and rRNA using riboPOOLs using customised probes for the marine sponge. All samples were subjected to the standard TruSeq Stranded Total RNA kits