Nanopore direct RNA seq
nanopore direct rna sequencing libraries were prepared from 1 ug total rna according to manufacturers instruction, using the direct rna sequencing barcoding kit (sqk-drb004.24). the final libraries (16-30 ng) were loaded onto individual promethion flow cells (flo-pro004ra) and sequenced until exhaustion (up to 72 hours) on a p2 solo using minknow software (v25.05.14).2