NFBFA, replicate 1, scRNAseq
Single-cell RNA sequencing was performed pre-infusion on CAR-T cell products from 8 patients (4 durable responders [DR], 4 non-durable responders [NDR]) using the 10x Genomics Chromium Controller. The workflow was modified to use either immediate fresh processing or thawing followed by sorting to improve RNA quality for 10x GEM formation and library preparation.