Amplicon sequencing of fungal ITS1 region from mulberry (Morus alba) plantation soil, Zigong, 0-10 cm, plot 3, replicate 2
Total genomic DNA extracted from soil; fungal ITS1 region amplified with ITS1F (CTTGGTCATTTAGAGGAAGTAA) / ITS2R (GCTGCGTTCTTCATCGATGC); barcoded amplicons pooled and sequenced paired-end (2x250 bp) on Illumina NovaSeq 6000.