DNA-SIP 12C-unlabeled control 4-chlorobiphenyl heavy fraction 12; water; replicate 2
Paired-end Illumina MiSeq sequencing of the V4-V5 region of the 16S rRNA gene amplified with primers 515F (GTGYCAGCMGCNGCGG) and 926R (CCGYCAATTYMTTTRAGTTT). Primer sequences are retained in the raw reads. Density class=heavy fraction 12; centrifugation run=1; SIP incubation=6 weeks; substrate=4-chlorobiphenyl; isotope=12C-unlabeled control; source treatment=water; source contaminant=Delor 103 polychlorinated biphenyl mixture.