Transcriptome sequencing of Aedes aegypti Aag2 cells
Total RNA was extracted using TRIzol Reagent (Invitrogen, USA) according to the manufacturer's instructions. RNA integrity and concentration were assessed using an Agilent 2100 Bioanalyzer. Sequencing libraries were prepared using the MGIEasy Fast RNA Library Prep Set (MGI Tech, China, Cat# 940-002921-00) following the manufacturer's protocol