Nanopore direct RNA seq
Nanopore direct RNA sequencing libraries were prepared from 1 ug total RNA according to manufacturers instruction, using the SQK-RNA004 kit. All of the final library (16--30 ng) was loaded onto a single PromethION flow cell (FLO-PRO004RA) and sequenced until exhaustion (up to 72 hours) on a P2 solo using MinKNOW software (v25.05.14).