16S rRNA V3-V4 amplicon sequencing of 2P-3
Soil bacterial communities were characterized by Illumina paired-end sequencing (2 x 250 bp) of the 16S rRNA gene V3-V4 region using primers 341F (CCTACGGGNGGCWGCAG) and 806R (GGACTACHVGGGTWTCTAAT). The samples were collected from continuously cropped tomato soils under different peat amendment treatments.