RNA-Seq of Mus musculus: retinal ganglion cells, Bcl2, replicate 3
Total RNA extracted from purified retinal ganglion cells was used for library preparation. Poly(A) mRNA was enriched using VAHTS mRNA Capture Beads (N401), followed by RNA fragmentation, first- and second-strand cDNA synthesis, adaptor ligation, size selection, and PCR amplification using the VAHTS Universal V8 RNA-seq Library Prep Kit for Illumina (NR605-02) according to the manufacturer's instructions. Indexed paired-end RNA sequencing libraries were generated for transcriptome profiling.