Camphora officinarum leaf RNA-seq at T0, biological replicate 2
Total RNA was extracted from Camphora officinarum leaf samples and assessed by agarose gel electrophoresis, NanoPhotometer spectrophotometry, Qubit 2.0 fluorometry, and an Agilent 2100 Bioanalyzer. Messenger RNA was enriched using mRNA Capture Beads and fragmented at high temperature. First-strand and second-strand cDNA were synthesized, followed by end repair, A-tailing, adapter ligation, size selection using Hieff NGS DNA Selection Beads, and PCR amplification. The resulting libraries were sequenced in paired-end mode.