RNA-Seq of mus musculus: adult female breast tumor
Single-cell suspensions were loaded onto the 10x Genomics Chromium platform for single-cell partitioning and barcoding. Reverse transcription and cDNA amplification were performed using the Chromium Single Cell 3' Gene Expression workflow. Single-cell 3' gene expression libraries were constructed using 10x Genomics Chromium Single Cell 3' v4 chemistry according to the manufacturer's instructions. Libraries were sequenced on an Illumina platform using paired-end sequencing.