Rapeseed eDNA
For all eDNA samples from both trials, each sample contained in a zip-lock bag was washed with approximately 0.5 L of distilled water and shaken for about one minute to release eDNA into the liquid. The wash water was then filtered through 0.45 m cellulose nitrate filters using Nalgene Single Use Analytical Filter Funnels (Thermo Fisher Scientific Inc., Waltham, USA) and a vacuum pump. In cases where filters became clogged (particularly for rapeseed tissue samples containing pollen and plant particles), additional filters were used.