GWAS of duoma sheep
1-1.5g genomic DNA was randomly fragmented by Covaris, the fragmented DNA was selected by Agencourt AMPure XP-Medium kit to an average size of 200-400bp. The selected fragments were through end-repair, 3adenylated, adapters-ligation, PCR Amplifying and the products were recovered by the AxyPrep Mag PCR clean up Kit. The double stranded PCR products were heat denatured and circularized by the splint oligo sequence.