Illumina 16S sequencing of mouse gut
Bacterial community profiling using the V4-V5 region of the 16S rRNA gene. Genomic DNA was extracted from mouse fecal samples. The V4-V5 region was amplified via PCR using primers 515F-Y/926R and sequenced on the Illumina MiSeq platform using 2 x 250 bp paired-end reads.