RNA-seq of Staphylococcus aureus in  invitro culture at 48 hours in wound like media (WLM)  replicate 2
Monoculture of Staphylococcus aureus LAC inoculated into wound like media in 96 well deep well plates grown aerobically at 37oC for 48Hours. At 48 hours, samples were collected and stored in Zymo DNA/RNA shield (Zymo Research R1100-250) for preservation for DNA and RNA extraction. The extracted RNA was prepared for sequencing using an Illumina Stranded Total RNA Prep Ligation Kit with Ribo-Zero Plus Microbiome (#20072063) with Illumina Unique Dual Indexes. Sequencing was performed on the Illumina NextSeq2000 platform using a 300-cycle flow cell kit to produce 2x150bp paired reads. 1-2% PhiX control was spiked into the run to support optimal base calling.