RNA-seq of mus musculus: gut iILC2s
iILC2s sorted from MLNs of IL-25-treated C57BL/6 mice were cultured with completed RPMI1640 containing 10 ng/mL of IL-2 and IL-7. Cultured cells were treated with either PBS with IL-25 plus IL-33 as a mock control or 20 mM spermidine with IL-25 plus IL-33 treatment for 16 h. Total RNA was isolated from the cultured cells by using miRNeasy Micro Kit (Qiagen). Total RNA were then reverse transcribed, amplified, tagmented and indexed according to the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina protocol. Two biological replicate samples were sequenced using an Illumina HiSeq 2500 with paired-end 150bp read length.