16S sequencing of mus musculus treated with penicillin
Fecal total genomic DNA was extracted using QIAamp Fast DNA Stool Mini Kit (QIAGEN, 51604) according to the manufacturers protocol. DNA was quantified using Qubit dsDNA Broad Range DNA assay (Life Technologies, Q32850). The variable regions 3 and 4 (V3V4) of the 16S rRNA gene was amplified by PCR using dual-index primers and sequenced on Illumina paired-end platform to generate 250 bp paired-end raw reads.