M14
Illumina PCR-free (tagmentation) libraries were made for the population. Barcoding was integrated into the library preparation using two plates of unique 96 Illumina IDT indices to enable multiplexing. Each library was then quantified using a Qubit, diluted, and all samples were pooled into a single sequencing library. The library quality was assessed using a TapeStation (Agilent, Santa Clara, CA) at North Carolina State Universitys Genome Sciences Laboratory and sequenced on an entire Illumina NovaSeq 6000 S4 flowcell with 100 bp PE reads. FASTQ files were demultiplexed by index plate and sample well to obtain sample-specific sequence data