RNA-seq of Seven member bacterial community in  invitro culture at 48 hours in wound like media (WLM) plus 4.5g/L glucose replicate 2
Seven-member community of Staphylococcus. aureus LAC, Streptococcus. agalactiae A909, Pseudomonas aeruginosa PA01, Finegoldia magna SY01, Cutibaterium. acnes SK137, Anaerococcus hydrogenalis MJR7738A and Corynebacterium amycolatum SK46 at ratio 2:2:1:10:10:10 inoculated into wound like media plus 4.5g/L glucose in 96 well deep well plates grown aerobically at 37oC for 48Hours. At 48 hours, samples were collected and stored in Zymo DNA/RNA shield (Zymo Research R1100-250) for preservation for DNA and RNA extraction. The extracted RNA was prepared for sequencing using an Illumina Stranded Total RNA Prep Ligation Kit with Ribo-Zero Plus Microbiome (#20072063) with Illumina Unique Dual Indexes. Sequencing was performed on the Illumina NovaSeq X platform using a 300-cycle flow cell kit to produce 2x150bp paired reads. 1-2% PhiX control was spiked into the run to support optimal base calling.