RNA-Seq of rice shoot
The total RNA extraction of seedlings treated with 140 mM NaCl for 0 h and 6 h, using the TRIzol reagent RNA extraction kit. mRNA was purified using oligo (dT) beads and fragmented, serving as the template for cDNA synthesis with random hexamers. After second-strand synthesis, the cDNA fragments were purified, end-repaired, A-tailed, and ligated to Illumina adapters. The libraries were then size-selected, PCR-amplified, and subjected to high-throughput sequencing using the Illumina NovaSeqTM 6000 platform (LC-Bio Technology CO., Ltd., Hangzhou, China). The measure of gene expression level was determined by the number of fragments per kilobase of transcript per million mapped fragments (FPKM). Differentially expressed genes (DEGs) were identified using the edgeR package in R (version 4.2.3), with a threshold of false discovery rate (FDR) < 0.05 and |Log2 (Fold Change)| > 7.