whole-genome resequencing of Formica transmontanis:YACC7W1
To extract genomic DNA from each ant, we first ground the ant tissue with a sterilized plastic pestle after immersing the tube in liquid nitrogen and digested the tissue overnight at 56C in 180L ATL buffer and 20L proteinase K. We performed the DNA purifying steps using the QIAamp 96 DNA kit for the QIAcube HT robot following the manufacturer's protocol. We eluted the samples in 100L of 10 M Tris-HCl (pH 8.0).   We prepared the samples for whole-genome resequencing using the Seqwell plexWell LP-384 library kit following manufacturers instructions. The libraries were sequenced on NovaSeq 6000 lanes by Novogene, Inc using 150 base-pair (bp) paired-end reads.