Viral metagenomic sequencing of Panthera uncia feces: XY-Feces-I1
Viral particles were enriched from snow leopard feces by 0.45-um filtration and DNase/RNase treatment. Total nucleic acids (DNA and RNA) were extracted using the QIAamp Viral RNA Mini Kit. RNA was reverse transcribed with SuperScript IV and random hexamers, followed by second-strand DNA synthesis with Klenow Fragment. Libraries were prepared using the Nextera XT DNA Sample Preparation Kit and sequenced on an Illumina NovaSeq 6000 with 150-bp paired-end reads and dual barcoding.