Whole genome Nanopore sequencing of Erwinia sp. Bb_ZA
Genomic DNA was extracted from a single individual of Bactrocera biguttulausing the Monarch Spin gDNA Extraction Kit (New England Biolabs, USA).Approximately 1g of genomic DNA was used for library preparation with the Native Barcoding Kit 24 V14 (SQK-NBD114.24, Oxford Nanopore Technologies) following the manufacturers instructions. Libraries were sequenced on R10.4.1 flow cells (Oxford Nanopore Technologies) for ~72h. Basecalling was performed using Dorado v0.9.1 in high-accuracy mode (Oxford Nanopore Technologies).