16S rRNA sequencing of dog feces: BRK11
Near-full-length 16S rRNA gene amplicon sequencing using a two-step PCR amplification method. The first PCR used UMI-primers (UMI_338ab_F/UMI_1391_R and UMI_27ab_F/UMI_1540_R) followed by AMPure XP bead purification. The second PCR incorporated custom barcodes (ONT-UMI16S SET1). Pooled libraries were prepared using the SQK-LSK114 kit.