0.2% colchicine + 2% DMSO treatment for 48 h
Total RNA was extracted from root tips of Phoebe bournei using PureLink Plant RNA Kit. DNA contamination was removed by RNase-Free DNase. RNA quality was detected via agarose gel electrophoresis and Nanodrop 1000. mRNA with poly(A) tail was enriched by Oligo(dT) magnetic beads, fragmented and reverse transcribed to synthesize double-stranded cDNA. After end repair, adapter ligation and PCR amplification, single-stranded circular DNA libraries were constructed. Paired-end transcriptome sequencing was performed on Illumina NovaSeq 6005 platform.