RNA-seq of Prunus persica 0 hours post-inoculation with Monilinia fructicola Mfrc123 (CBS 144850), replicate  3
Healthy Prunus persica cv. Tirrenia fruits were surface sterilised, wounded with a sterile needle to a depth of 5 mm, and inoculated with 50 L of a conidial suspension (1  10^6 conidia mL1) of Monilinia fructicola Mfrc123 (CBS 144850). Fruits were placed on sterilised Nestipacks and incubated in a moist chamber at 251C in the dark. Samples were collected at 0 hours post-inoculation (hpi) from tissue surrounding the inoculation points. Total RNA was extracted using a modified cetyltrimethylammonium bromide (CTAB)-based protocol adapted from Meisel et al. (2005; doi: 10.4067/s0716-97602005000100010). RNA was sequenced on an Illumina NovaSeq 6000 platform, generating 100 bp paired-end reads.