RNA-seq of Seven member bacterial community  in C57BL/6J murine wounds at four days post infection replicate 3
Seven-member community of Staphylococcus. aureus LAC, Streptococcus. agalactiae A909, Pseudomonas aeruginosa PA01, Finegoldia magna SY01, Cutibaterium. acnes SK137, Anaerococcus hydrogenalis MJR7738A and Corynebacterium amycolatum SK46 at ratio 2:2:1:10:10:10   infected into dorsal skin wound of 8 to 10 weeks old female C57BL/6J mice and harvested at four days post infection. At 4 days post infection (4dpi), the wound tissues were excised and immediately saved in 750ul of Zymo DNA/RNA shield (Zymo Research R1100-250) for preservation for DNA and RNA extraction. RNA extraction was performed using ZymoBIOMICS DNA/RNA Miniprep Kit (R2002). The extracted RNA was prepared for sequencing using an Illumina Stranded Total RNA Prep Ligation Kit with Ribo-Zero Plus Microbiome (#20072063) with Illumina Unique Dual Indexes. Sequencing was performed on the Illumina NovaSeq X platform using a 300-cycle flow cell kit to produce 2x150bp paired reads. 1-2% PhiX control was spiked into the run to support optimal base calling.