16S V3V4 of human vaginal metagenome: Symptomatic VVC
DNA was extracted using the Qiagen DNeasy 96 PowerSoil Pro kit on a Qiacube HT instrument. The V3-V4 regions of the 16S rRNA gene were amplified using Illumina-tailed primers (Pro341F: CCTACGGGNBGCASCAG; Pro805R: GACTACNVGGGTATCTAATCC) and sequenced on an Illumina MiSeq platform using Chemistry V3 with a 300PE strategy.