RNA-seq of Four member bacterial community  in C57BL/6J murine wounds at four days post infection replicate 3
Four-member community of Staphylococcus aureus LAC, Pseudomonas aeruginosa PA01, Enterococcus faecalis V583 and Finegoldia magna  SY01 infected into dorsal skin wound of 8 to 10 weeks old female C57BL/6J mice and harvested at four days post infection. At 4 days post infection (4dpi), the wound tissues were excised and immediately saved in 750ul of Zymo DNA/RNA shield (Zymo Research R1100-250) for preservation for DNA and RNA extraction. RNA extraction was performed using ZymoBIOMICS DNA/RNA Miniprep Kit (R2002). The extracted RNA was prepared for sequencing using an Illumina Stranded Total RNA Prep Ligation Kit with Ribo-Zero Plus Microbiome (#20072063) with Illumina Unique Dual Indexes. Sequencing was performed on the Illumina NovaSeq X platform using a 300-cycle flow cell kit to produce 2x150bp paired reads. 1-2% PhiX control was spiked into the run to support optimal base calling.