library construction protocol
Combined DNA and RNA were extracted from slurry with a phenol-chloroform protocol. For RNA samples cDNA was synthesized. PCRs were performed using primers with unique 8 bp barcodes at forward and reverse primers. Multiple samples were pooled into one library for sequencing. During demultiplexing reads were sorted by read direction forward-reverse or reverse-forward using the primer sequence, resulting in multiple paired fastq files (=runs) for the same ENA experiment
library name
Lib22
sequence lane
HKMLNDRXY_L1
read direction
reverse-forward
ENA-FIRST-PUBLIC
2024-02-22
ENA-LAST-UPDATE
2024-02-22