attributes.tag: library construction protocol
attributes.value: Combined DNA and RNA were extracted from slurry with a phenol-chloroform protocol. For RNA samples cDNA was synthesized. PCRs were performed using primers with unique 8 bp barcodes at forward and reverse primers. Multiple samples were pooled into one library for sequencing. During demultiplexing reads were sorted by read direction forward-reverse or reverse-forward using the primer sequence, resulting in multiple paired fastq files (=runs) for the same ENA experiment
attributes.tag: library name
attributes.value: Lib23
attributes.tag: sequence lane
attributes.value: HKMLNDRXY_L1
attributes.tag: read direction
attributes.value: reverse-forward
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2024-06-12
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2024-06-12