NextSeq 500 paired end sequencing; RNA-seq of human cerebromicrovascular endothelial cell line hCMEC/D3 or primary human brain vascular pericytes stimulated with TNF, or S. pneumoniae, in monoculture or in coculture, or stimulated with conditioned media from monocyte-derived macrophages (MDM) stimulated with S. pneumoniae, all compared to unstimulated controls