library_design
Libraries were prepared with 16/18S rRNA gene primers 515F-Y and 926R (Parada et al. 2016), 30 cycle PCR with KAPA HiFi HotStart Ready Mix. For each sample, triplicate PCR reactions were pooled and cleaned (Gene Clean kit, MP Biomedicals). Samples were indexed, purified (Agencourt XP Beads, Beckman Coulter), quantified, pooled in equimolar ratio, and sequenced with MiSeq 2 x 300 bp, v3 chemistry (Illumina).
ENA-FIRST-PUBLIC
2024-07-06
ENA-LAST-UPDATE
2024-07-06