library construction protocol
DNA was extracted from sediment samples with a phenol-chloroform protocol. PCRs were performed using primers with unique 8 bp barcodes at forward and reverse primers. Multiple samples were pooled into one library for sequencing. Some samples were sequenced on multiple sequence lanes and libraries in order to retrieve sufficient reads; during demultiplexing reads were sorted by read direction forward-reverse or reverse-forward using the primer sequence, resulting in multiple paired fastq files (=runs) for the same ENA experiment
library name
Lib7
sequence lane
HHMW5DRXX_L1
read direction
reverse-forward
ENA-STATUS-ID
4
ENA-FIRST-PUBLIC
2025-07-24
ENA-LAST-UPDATE
2025-07-24